LOCUS       DAEPIN010000001       134486 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.001, whole genome shotgun sequence.
ACCESSION   DAEPIN010000001 DAEPIN010000000
VERSION     DAEPIN010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 134486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 134486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..134486
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.001"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            46..120
                     /locus_tag="KR250_000001"
     tRNA            46..120
                     /locus_tag="KR250_000001"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:78..80,aa:Thr,seq:tgt)
     gene            complement(280..957)
                     /gene="srtB"
                     /locus_tag="KR250_000002"
     CDS             complement(280..957)
                     /gene="srtB"
                     /locus_tag="KR250_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase SrtB"
                     /protein_id="HBH0065976.1"
                     /translation="MKKLYRIVINIILVLVILYSGFNIYSKLTKYNHDTKISSELQKK
                     EYKKEDLSKINSDFKFWLSVENTNINYPVVQSKDNSYYLDKDFYKKDSISGTLFMDYR
                     NKSIDDKNIIIYGHNMKNKTMFNNLNKFKDADFFKKNNKIKITLNGKEFLYDVFSAYI
                     VESDYDYLKTNFNNESDYQNYINDITSKSLYKSPIKVNSNDKIVTLSTCTYEFDDARM
                     VIHGRLI"
     gene            1089..1423
                     /gene="rnpB"
                     /locus_tag="KR250_000003"
     ncRNA           1089..1423
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="KR250_000003"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            1701..4244
                     /locus_tag="KR250_000004"
     CDS             1701..4244
                     /locus_tag="KR250_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HBH0065977.1"
                     /translation="MKVLSENNIIRNTVLNVTASFLKQESKINEKLDGVLEKKFEKVE
                     FNEAKYAELLKFNILFYKTLARNTEPLIGKWIVDKYIPEIDELEKDLELTTAKCRKYV
                     NKAMKEGLDSLKANDLNSFLAYDKMELSERRRRLEKDYKVLNLYKDLLNISLRKISLE
                     KKECGDLFLKNQADAKRELKREIIFCVNKILASNKDVVPNNVEENNEQNKVEIKATEI
                     EDLQLGKDNSENDMLLNEIKNSKVKHESKSNDIESKFIDKLNSLNNESISQTIHNEYK
                     KLCGLEELHSVEAYGFGKEIIKDFACATVVLEFLKRRNRDLIEGAMRLTIIGEFGPEN
                     FKEFVDYVIKNKTEISEDTWNKAQNLIKDNYSELENHEIASKRTRRNKDIDIEEYIYM
                     IKNADKDICFRSSISIEDDAEEGTKEEVDSNNQDIGDVVEDKDTTDKEYDSNKEDIIE
                     PENKKSKKKAKLFGFIKKDNEEAEQEEENLNDISPDIILDKPVENHQVKSEEIEQNEL
                     KEIKQEEPSQHIEEERSVKIEKPINNNLDEKVSSNNESKLEKESKNLEDKKAKEIKEE
                     KLENEKSVVIPIKKKENSNKKSKNSSKDKYRENKKEMKNYVSDKEDSLDDEEVVSKKS
                     RLKETIIAVVIVVIVGVGYFITVGNNKKNDKENIPKSSTQQQANNKLTEEEKKAQAEK
                     EKKEAEEKAKAEKEAQEKAQAEKKAKEMEAYKDGKGVYYTVYAGSLKVEKTAKETAKE
                     YEAKGISSTIIQENGYYKIKIGDYSQYGEAQEKCNELAKKSIDTYIAMYDKYYDYKLE
                     ELKESAPSLSAEELKQKYEDLRSELKNKSGYREYVKHLDKLYEEIVEGA"
     gene            4367..6358
                     /locus_tag="KR250_000005"
     CDS             4367..6358
                     /locus_tag="KR250_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0065978.1"
                     /translation="MLKLGEKIIYELSDGFELSGISRYSKEYKKLHITNLGNIIISKS
                     DEVNEDTGVKYIYSFSDNKEKLEAVLEDSQIIVYGNNLPFYIKSENSSKNVTANLTMK
                     ITLDEYDLICKSQREFIFYLKDNMTILSLDDNKFYMGGINKDNEKFIFISGKNRFEIN
                     FDDIERYILEDKRVSLKGYFHMEREGIIVRSVSIFNNNIDSVVPSDLNERVKDNQKIG
                     NLPKDCEIVFCKISGNIDGFDYKNTNMLLVKYQDQLIFINKKSKKTIVKSAKDNCSKL
                     NLGEDIILYDNKNVFNLHINDKNREIMQIDDLKDIENEIVGYTLKHAPFFIQEDFDSL
                     TILKSFQKEIISIKNSDIKDIVINKELENENSNFVETEIKFNNQKVLLNLSKSMVQKL
                     MQDVFIYAKQPLLKENSIEVIYKNWSKAMNDMIIFNFFGNIYYMKSEFDKILEKELND
                     EIRIEVINSLYKQIQEQRNNLDLLSAYMPRILENQEIDLFEKYNTKLDVQVFKQIKNL
                     LSDLSYNISSYLNEVEKSLDNIIFVISGEDKKKYNYRMLKESESASLDVFLKQAISRL
                     NHLVENMYPYYVDETSREMFKLFELLWKNYRNIDDDSIKEILFERITNTYVFKQLTLN
                     NSTKERRKDIIEKIYNSVDYGTNKLDENMFFTGGIKYVK"
     gene            6348..6668
                     /locus_tag="KR250_000006"
     CDS             6348..6668
                     /locus_tag="KR250_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0065979.1"
                     /translation="MSSKYNVWTFNYEFLGLDSGDEKNNEVYYEIDLDEILNENYIDE
                     DIDSGLDSDGNSILGELISEMDIDEDVSVDMTYQELEVDLEDLDSYLDEDIDSDIKGI
                     LDEI"
     gene            6866..7735
                     /gene="galU"
                     /locus_tag="KR250_000007"
     CDS             6866..7735
                     /gene="galU"
                     /locus_tag="KR250_000007"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HBH0065980.1"
                     /translation="MTVKKAIIPAAGLGTRFLPATKSQPKEMLPIVDKPTLQYIIEEA
                     IESGIEEILIITGRNKKSIEDHFDKSVELELELEQKGKTEMLEMVRDISNMVNIHYIR
                     QKEPKGLGHAIYCAKSFIGDEPFAVLLGDDIVDSDVPCLKQLIDTYNEYKTTVLGVQK
                     IAKEDTNKYGILDVKHIEDRVYKVKDMVEKPAIEEAPSDIAILGRYIITPAIFSILEK
                     QTPGKGGEIQLTDALQTLGKQEAIYAYNFEGRRYDVGDKIGFLEATIDFALKRENLKD
                     DLMDYMRKKVSEK"
     gene            7759..8772
                     /gene="galE"
                     /locus_tag="KR250_000008"
     CDS             7759..8772
                     /gene="galE"
                     /locus_tag="KR250_000008"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HBH0065981.1"
                     /translation="MAVLVAGGAGYIGSHTAIELLESGYEVVIVDNLSNSNSIVVDRI
                     KELSKKPVKFYNIDIRNKDEMHVVFKENNIESIIHFAALKAVGESVEKPIEYYSNNLI
                     STLNLFELMREYGVKKFVFSSSATVYGDPHTCPILEDFPLSVTNPYGRTKLMIEQMLV
                     DISKADKSLDIALLRYFNPVGAHKSGRIGEEPNGVPSNLMPYITKIAVGKLKELSVYG
                     NDYPTHDGTGVRDYIHVLDLAAGHVKALQKLEENPGLVVYNLGTGKGYSVLDLVKAFS
                     KASGKEIPYKIVGRRAGDVAMCYADSSKAEKELGWKAKYELEEMCEDSWRWQSMNPNG
                     YEE"
     gene            8884..10818
                     /locus_tag="KR250_000009"
     CDS             8884..10818
                     /locus_tag="KR250_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp22"
                     /protein_id="HBH0065982.1"
                     /translation="MKKSLKKYLVLALTLVLVFACGVPESSAASKHVIIVNSRKNTLG
                     YFVNNKLVKEFRVATGKKGSETPTGKTKVVNKIKNRPYYKGNIPGGSPRNPLGDRWMG
                     LALKGTYGDTYGIHGNNNESSIGKHISGGCIRMHNKDVRWLFDQVPVGSDVIIDYSND
                     SYVKIAAKYKINLNQTGWKTENGKKYYVKSDGTYQKNSWLKVNGKMYYFDASGVMQTG
                     WKTINNKKYYLGTDGARVSGWKVIDGKTYYFNSDGVMQTGWQEKNGKKYYLGSDGLAV
                     TGWQEIDGNKYYFDKTGIMQTGWQQIDGKSYYLDKDGKMLTGSQKIDGKDYTFNEDGT
                     INPTWDTIIGANRFDTAKKISSVGNWNADSSDTVILVNGNAIADGITATPLASSYDST
                     ILLTNTANLPTETVEEMKLLAPKTVILIGGENAISSKLEQEIKTTFNTETKRIAGQDR
                     YQTATRIAEELGNREEIKTAYMVSGNGEADALSVASKAGEEKQPIILVNKDGITEESY
                     KWLTERKLENAYFIGGPSAINDSVIAKMNDITTEDISGNRIYGDSRVDTNAKVIEKFY
                     GDTDLQAVLVSKSDALVDALSAGPLAVKLHSPIVLMDNSGLSSEQQRVFANKKVETPY
                     QIGGGVSYIVMDKLMDILAK"
     gene            10973..11836
                     /locus_tag="KR250_000010"
     CDS             10973..11836
                     /locus_tag="KR250_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HBH0065983.1"
                     /translation="MLSSYVKRVSKIIKKKYIMFIDESGRADINHEDPFTLTGVIFEY
                     KYAVSQGDYVCSLRKEMDAFKEYCFGTSNIGIHLTDISRKSRAFEKFDDSQIKLFYDE
                     LPLFLSRLECTIISVTVDKARLKKYYAPSKEPYVVAFIHVLQNFYSFVNNETVESARI
                     VIESRDDVSNLKVQKAFFDVFNNGTIYLDIDEELRDKVKGFIIAKKEDADYREGLEIA
                     DILCNPLSRARRGLIEANPKCMQYGEKNKIFKSVRNKIYTPTSVNDIRNWGFKEVPIV
                     EPVGPWLEKMR"
     gene            complement(12044..12925)
                     /locus_tag="KR250_000011"
     CDS             complement(12044..12925)
                     /locus_tag="KR250_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBH0065984.1"
                     /translation="MNLYHLRYFVTLAHLEHYTKAAENLSITQPSLSHAISLLENELG
                     VALFEKEGRNIVLTKYGKIFLKDVEKSLEILDSSVKSLKITGTGEGQIDLAFLRTLGT
                     DFIPDIVHKFLKSNPAKSIDFKFHTGVTTDIIQGLKERKYDIAFCSKLEKEKGIEFIP
                     VAKQDLVLIVPYSHPLAAKDTIDLKETIPYPQIVFNQRSGLRYIIDDMFKKINQQPNI
                     VYEVEEDQVIAGLVAKNFGIAVVPNMNMLSFTKVKVIQIIHPSWERNFYLAFIKDRYL
                     PPAIKNFKNFVIKNAQL"
     gene            13338..14186
                     /locus_tag="KR250_000012"
     CDS             13338..14186
                     /locus_tag="KR250_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HBH0065985.1"
                     /translation="MNNQKNLNIPITISSYTLGTEVSFRDRVRIAKEAGFDGIGLRAE
                     NYIDAKNAGVTDEEMIAILNEYDMKVTEVEYITQWGREEDRTEAQREKEQNVYHMAHL
                     FNVKHINCGLLEMIPEEQIITALGELCDRAGELIIGLEFMPYSGVPDLATAWRVVKGC
                     NRENAMLILDTWHWARAKQTAEDLAPVPADKIVSIQVCDVLETPYEKLRDESLHDRLA
                     PGEGYGNTEEFVKIIKEHNISPRVIGAEVISDSLISKGLSTAAETVFNATKKVLDKAW
                     SEVSPK"
     gene            14250..16172
                     /locus_tag="KR250_000013"
     CDS             14250..16172
                     /locus_tag="KR250_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBH0065986.1"
                     /translation="MKISSMFTPIRIGSMTVPNRFVVPPMGNNFANTDGTLSETSKAY
                     YLERALGGFGLITIESSVVDKKAKGGPRKPCLYDDSTIDSFKNVIDACHDAGSKVSIQ
                     LQHAGSEGNEKVAGHPLKAASAIPASNGRNTPLAITTEEIYELIESYGDAALRAQKAG
                     ADAVEVHCAHGYLVSSFISQRTNKRVDEFGGCFENRMRLPRLIIENIRKKVGNSLAIL
                     CRINSTDDVPGGIDVHDSSVIAAYLEDCGIDGLHVSRSIHIHDEYMWAPTTLHAGFSA
                     ELVTEIKKAVSIPVITVGRYTEPQFAELMVRQGRCDLVAFGRQSLADPEMPNKAKNGK
                     LDEMIPCIACLQGCVPNMFQGKPIACLANPILGHEAELKPAEISKEVLVVGGGVGGML
                     AAWVCAKRGHNVTLVEKSEVLGGQMRLAAYPPGKGDITNLVRSYISKCNQYGVKICTN
                     TEATVELIKEKSPDVVIIATGATPLVLPIPGINDSGLIHAVDLLDGKKSCGKKVLVVG
                     GGMVGCEVAAFLGEQEHEVTVIELREEVGADVISEHRKFLMNDFENYNIHTITGAKVS
                     KFYDDGVDYLLTDGTEHDLKGFDSVVLAMGSRNYDPLSEVIKEVVKETYIVGDAVKAR
                     RALDATKEAFEVALNI"
     gene            16254..17117
                     /gene="aroE"
                     /locus_tag="KR250_000014"
     CDS             16254..17117
                     /gene="aroE"
                     /locus_tag="KR250_000014"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HBH0065987.1"
                     /translation="MEISGRTGLFALIGTPVGHSKSPVMYNYSFKKLDLDYRYLAFDI
                     TVDKVKEALLAIKTFNIKGANVTMPCKSAVTEYMDELSPAARIIGACNTIVNDNGKLV
                     GHITDGVGYVRNLKENGVEVKGKKITIMGAGGAATAIQVQCALDGAREISIFNPKDDF
                     YKRAEQTVENIKKDVPECVVNLYDLEDTNKLYEEIQSSDILTNATLIGMKPYDNETNI
                     KDTSVLRKDLVVTDVVYNPKKTKMIEDAEANGCKAIGGLGMLLYQGAEAFNLYTGLEM
                     PVEEVNELCFK"
     gene            17317..18534
                     /locus_tag="KR250_000015"
     CDS             17317..18534
                     /locus_tag="KR250_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBH0065988.1"
                     /translation="MNNKKYYPTAIALYFSYFLLGIGISILGQYKPEFSSMWGAKTLS
                     DGTLDVSIVLAVIAALGLGRLISYPFAGPISDKYGRKVSGLIGNFLHAIFFVGIVFSP
                     NFYIAYVFAIIGGAANSFLDTCVTPSCMEIFASLGTIANMFTKFTIALAQFLLPFIIG
                     FVAANSISFKVIFIVTAILIVVDAFLIAVLPFPPANNVIDNKGKTVKSEKMKFTPTSI
                     ALVCIGFTCTSTFVLWLNCNQELGKLYGMADPTKIQSFYSMGVICAVLITSLLIKKYI
                     KPIRILVIYPIIALLMLLVVYFVQTPTICMLGGFVIGYSAAGGVLQLTTSTANEMFPT
                     NKGKITSIVMIASSIANYVILNIAGIITKSGGVNGPKYVVLFNVAITFVGILLALFVN
                     MRYEKEKVYDYDV"
     gene            18770..20635
                     /locus_tag="KR250_000016"
     CDS             18770..20635
                     /locus_tag="KR250_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIRAN domain-containing protein"
                     /protein_id="HBH0065989.1"
                     /translation="MENVFKRLQEFNGYDGYKESFEMNYLCIYESIPLREQVELANNL
                     VDEILNMYKSESNEIYLLEGSNSKSLICYFEIFMKKINTLVKEMIIDEKWLYKLTKEL
                     IYKSKKVEYVKLGLVLSEKYLNVENLREVVDTFSKSGEYVFYLSNTIKKLEFYNTYLF
                     NLSKKATGSIKVFAIVNMENLDSKINSYLIEDGYKDTKYERLLMNYIISIVDLNEYLE
                     KRDLDKEKINNLARLICNYLLSVEFKYIGNKLELVNRFLPTVVNYGTNFESLYSIFLI
                     AINVLKDENIECNKIEFEKEINGILLSEKWKNIYFEALRDASGKTEDIIKMSEIYDVN
                     LSFDDLLPYLNRDIRDFEVYWHISKKGTTSSRLKLLNFFEETFKIDDLIGKMKDIEKD
                     KLTQEYYDDMLFFIVLKGSKSLYPEGKNISLKGIFGNINEVRKESINILKRYREKLSL
                     EELKIVKEAYEKEKNVILKDELRRVLYESNNLKKEFVNIEKIKVDEHGKDIYLTSIAV
                     AGSRFRNREYLEKELEKSKIYYLTREKDNLYDEKAIKIVGETGYVIGYVPRKENYILS
                     NLLDGGKLLYCRVTEYNLYEDCIYANVYLSYKDVIETVENSLKMVLDKSRIKLIN"
     gene            complement(20813..22177)
                     /locus_tag="KR250_000017"
     CDS             complement(20813..22177)
                     /locus_tag="KR250_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBH0065990.1"
                     /translation="MSQLLIKNAYLITMNADREVYENGSILIENNIIKEVGKFDINIV
                     DKDAEIYDAKGKILMPGLVNTHVHLSQQLGRGLADDVVLLTWLRERIWPYESSFNYED
                     SLISSTACCVELIKSGVTTFLESGGQYVDAMVEAVDKTGLRACLAKSVMDTGDGLPEA
                     WQKTTDEEINTQLDLFHKFNNTSNERIKIWFALRTIFNTTEELLVRTKELADKYNTGI
                     HMHIAEIADEISFVKQNNGVGTVEYLDKLGVLGPNLLAAHTVWLTHKEIDLFRLHDVK
                     VSHDPGSAMKVVLGFASIPEMLEKGIPVSIGTDGAPSNNRMDLMRDMYLTSLIHKGRT
                     LNPTVVPAEEVLEMATINGAKCALLEKEIGSLEVGKKADMIILNPDTIHCLPMHNPIG
                     NIVYSMTSENVDSTICDGKWLMKERKVLVVDESELLNKVKKQASKIKDKAEVKIPSKF
                     KIIK"
     gene            complement(22356..23699)
                     /locus_tag="KR250_000018"
     CDS             complement(22356..23699)
                     /locus_tag="KR250_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HBH0065991.1"
                     /translation="MVPNTNNVQPGGNNSSLSFLDKMFKFSERGSSLKTEVIGGVTTF
                     LTMAYIVFVNPAILSEAGMDKAALITVTILATAIGTLIFAILGNAPFALAPGMGLNAF
                     FTYSLVIGAGIPWQTGLGVVFLSGCVFFTLAVTGLRKTIADAIPRELAVASSAGIGLF
                     LAFVGLKNMGVIIANPDTIVSLADFTPTVLIALFALILMAILEYKKVKGGILISIVVA
                     TIISVIFGFVELPTAIISTPPSIAPVAFKLDIVGALKFSLIGPIFSFMFIDMFDSLAF
                     LITCCKQMGLEDENGEIKDLGRMLYADVSSTLIGSFLGTSTVTTFGESASGIAAGART
                     GLASIVTAALFLATLIFTPLLGIVPTYAAAPALVMVGVFMFESIKQIDFADSKIAVPA
                     FVTVLLMPLTYSISIGLCFGFISYIIMHVVAKETDKISITLWIIGLLAVVNLVLS"
     regulatory      complement(23772..23873)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            24202..24855
                     /locus_tag="KR250_000019"
     CDS             24202..24855
                     /locus_tag="KR250_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBH0065992.1"
                     /translation="MTKIIKVQKPKFTGELEIIENLEDILEDIFNDEKIFNKIIENEV
                     ISDLDSVRVSFESCIFRNVSFENCNLKKIDLLDVVFENCNLSNTFFDGGSIYRVEFKN
                     SKLIGVRFDDCILKNVLFKDVLGKYSNFSFSKFKIVSILDSNFENSVFQEVKGDNLVF
                     EYTNLKKANFNGTKLSGIDFTTNNIEGIEIGIDDIRGAIFDVSQAIDLTKLMGIIIK"
     gene            24996..26267
                     /gene="brnQ"
                     /locus_tag="KR250_000020"
     CDS             24996..26267
                     /gene="brnQ"
                     /locus_tag="KR250_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HBH0065993.1"
                     /translation="MRDIFIVGFALFSMFFGAGNLMFPPFLGMESGKDWLIPLIGFVV
                     ADAGIAMLVIIATARCKGSMDDVLRRAGKGLARVMSVAALACLALLVIPRTCATTYEM
                     GIMPIFGDHGPVAKAVFSIIFFGLTLAFTIRSSKVIDIIGKYLTPALLLVLFVLIVKG
                     IISPAGPMSPEHMIDKNLFGEGISQGYQTMDALGAASMATIMLMSIIAKGYTSDKDQI
                     GMTIKAGFVACVFLAVVYGGLTYLGATVSTLYDTSIPQASLLVEITSILLYGQVGKVI
                     LGVIVALACLTTSTGLTASISTYFEGISKASYKQLVIGICVASMLISNLGVDSIIAIS
                     VPILQTIYPVLLAIVVMELAGKHIKNDNAFKGAAYVTLVISLLSAINGMTGAVPFIQS
                     LPLAGLGFNWVIPAILGGIVGNFIKSNKQVA"
     gene            26469..27656
                     /locus_tag="KR250_000021"
     CDS             26469..27656
                     /locus_tag="KR250_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3798 domain-containing protein"
                     /protein_id="HBH0065994.1"
                     /translation="MLRKITAFMLSICLIIGLTGCSLLVGKKDKEKIIDDFKVAVVTQ
                     PLSENKVQYNMVEEMAKEYEEENKIDKDKDGQTKVKQTIKHVVLPENFTSNIDSAINK
                     IVKLADDKEVQAIVVSTDQAGLLPALQKVKEKRPEIITISAPMGDDKNQLSQFVDVNL
                     GVSAEERGKVLAERSKEMGAKAFIHYASTDDLKDVNIAKRLEMIKETCKNIGLPFVQV
                     NTPNINTEEDKNKVKQFLNEDIEKQVKKYGKDINVFGVNEYMDEVILTKALELKYIVA
                     EQSNPSPIQTYPSVMGLKISEKDAQNYDKINDMISEKAKAFGMSNRLGGYPMPMDAFL
                     PSVAIYLATEMVKQDLTQEDVCDPDYLEAFTELRFGIGSEFTPLTEVLYNYQSVILSQ
                     LIY"
     gene            complement(27886..29910)
                     /locus_tag="KR250_000022"
     CDS             complement(27886..29910)
                     /locus_tag="KR250_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBH0065995.1"
                     /translation="MDKKKLILITHDKLNSDHYKEELTNFFGDEIIIETQNILDGIKE
                     NLEGDVVLSLSPLTSNFLIKHFKEDIEIIHGTKALSKLGYEKMMKLPPGTKSLLMTTN
                     KTSAFEMATYLYKIGINHIDFVPTYPDCDEIYDLDTAITPGQIRFIPKYIKNIVDLGW
                     RKISLDTYMSLLVVLKLKNEKFIEKLYKLSKETLSHDFLNTSLDNISKLKTILYMTID
                     EIGDGLIFFNTFNKVTFVNKSLLNMLGLDEKLIKSPSLMEYMPNSFLDKITKNLNIDN
                     MIIYIDEIDKKFILSKKPFYLYKNIEGCLITLKDVNNIEILEQKIRSDSVKRGYVAKY
                     KFNNIIGNSSIIKDCIKKAKKMALTDNPILITGETGTGKEAFTQSIHNHSNRKNKPFV
                     AINCASLPSELLESELFGYEDGSFTGAKKGGKKGLFELAHTGTIFLDEIGDMPHDLQV
                     KLLRVLQEKEIRKIGGTSIIPIDVRILAATNKDLEKLIIENKFRMDLFYRISMFTLDL
                     PPLRKRLEDIPLLLESFLKELPYKNIKLDKSLLEALNSYTWMGNIRELRNCIEYMAYM
                     GSNYLTINDLPQNISSKLNNNHMSSNMSIFNDLNQYDKNICISILKSLHMKPMGRTKL
                     MKFMEYNVTEYEVRNMLEYLTRNGYLISSKGRKGSSITEKGIKIIENNII"
     gene            30300..30968
                     /locus_tag="KR250_000023"
     CDS             30300..30968
                     /locus_tag="KR250_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0065996.1"
                     /translation="MSKLESTTEKVEVKWYGYVALILGALFFSGIFKDAPGPLKVLDF
                     NNVLGSFGTLGTINDGVGTLAANFRGDGGTGPRDGWLYALTLIPSVMFALGVVRVIDH
                     LDGMKAAQKLLSPLLKPLLGLPGFAGLTLIASLQSTDAAASMTKELKDDGYIDEKQKA
                     VFCAFQFSGASAITNFFASGAALFPFIGDVPIFIPLALILIMKFVGANLLRLYLNKFD
                     KGEA"
     gene            30970..31455
                     /locus_tag="KR250_000024"
     CDS             30970..31455
                     /locus_tag="KR250_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HBH0065997.1"
                     /translation="MSEVAKKEVKVKKGNVIDAFIGGARNGFQISTNSMAPNVIFGFA
                     IISVFNLTGLLDVIGTIFTPVMSIFGLPGVAATAIMTIFISMGGASGVIAGLFTAGQI
                     SQAHVAILLPMLLLSGAQIQFMGRLLGTAELKTKYYPHMFVIATLNGCIAMLIMNYFV
                     V"
     gene            31516..32637
                     /locus_tag="KR250_000025"
     CDS             31516..32637
                     /locus_tag="KR250_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 peptidase aminoacylase family protein"
                     /protein_id="HBH0065998.1"
                     /translation="METNKDYIEYVHKVYDYLHTIPEKGFEEYKTSEFVLKELEKMGF
                     ENINKGIAGTGIIVTIDSKKEGPVFAIRADMDALEFIIDGEKKMIHACGHDGHTSMLL
                     TAAKLIKERNLVKRGKLKILFQPGEEKLYGALRVIDSGLLDDVEEMVGMHLRPGQDCR
                     LGKAIPAMYHCASCIMEFTIKGITCHGSKPHLGVNAVDAVCLAVNAINAIRVNPAIPN
                     SIKVTNISVKGETYNNIPDEAFMALDIRAESNDIIEELKEKAIHAVKTAVSSINAEAV
                     LISKDGVPAAEFDEELVNLAEETIKDVLGADASIGIFKNSGGEDFHYYTQKLKCKTTY
                     IGLGADATPGFHNPNVTFDTKALEYGVDIWCRLVEKRLG"
     gene            32918..34108
                     /locus_tag="KR250_000026"
     CDS             32918..34108
                     /locus_tag="KR250_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBH0065999.1"
                     /translation="MSKYNFDRVIDRVGTDCVKWDFRTNCSTKAQKDGLPFWIADMDF
                     ECAEPIIEALRKKVEHKIFGYSSNDSDKYFNAVCDWYKRRFNWEINRKDIIFSPGVVP
                     AVAILVRILTNSNEGVIVQKPVYYPFEAKIKSNNRDVVNNPLIYENGTYRMDYDDLEE
                     KAKCSNNKVLILCSPHNPVGRVWREDELKKVVEICKKYDLWIIADEIHSDLIRKGFKH
                     TPLQSLCPEYKDKIVTCIAPSKTFNLAGMQLSNIIVNNDELKKKYQEEVTAVGVATSP
                     NPFAIVATIAAYNESEDWLNELNDYLDNNIQFIDEYLKENLPKVKLVYPEGTYLAWLD
                     FTAYGLNEVELEDLMFKKANVLFDEGYIFGKEGIGFERINVACPQSLLKECMDRLKTI
                     FENL"
     misc_binding    34242..34497
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            34556..35557
                     /locus_tag="KR250_000027"
     CDS             34556..35557
                     /locus_tag="KR250_000027"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HBH0066000.1"
                     /translation="MSTIIPKDYKSSLNVIDTQIAIKKLKDFFEMRLSNELNLIRVSS
                     PLFVLPETGANDNLNGEKAVSFDIPFMNKNAEIVQSLAKWKRLALKKYGFEVGSGLYT
                     DMNAIRKNEELDNIHSLYVDQWDWELVIDKSSRNEKTLKDVVKRLYGVFKDTEIFVCS
                     MYEGIKEILPEEITFITSQELEDMYPELSPKEREDKIVKEKKAVFITQIGKTLISGEK
                     HDGRSPDYDDWELNGDILFYNPVLDSALELSSMGIRVDEESLDRQLKLAGCEERKEFD
                     YHKMLLNGELPYTIGGGIGQSRICMYFLQKAHIGEVQVGVWPQDMIQNCSSAGIELL"
     gene            35661..36050
                     /locus_tag="KR250_000028"
     CDS             35661..36050
                     /locus_tag="KR250_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HBH0066001.1"
                     /translation="MEFLKIIINIVLDILKKILVRFKNAKFGLVFVFDLLKLPDFMTD
                     KRINIVDKIKVISVLIFTISYFVSGVDIIPEMIAGAFGFIDDAIVLIWSIGIVNEEIN
                     KYRVIAKKDKHSNIIENVEFSIKDEEE"
     gene            36211..37419
                     /locus_tag="KR250_000029"
     CDS             36211..37419
                     /locus_tag="KR250_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HBH0066002.1"
                     /translation="MKKKIGLVPKLIIGIIVGILIGSYAPEIIVQILVTVSTLFSAFL
                     KFVIPFIIIGFVTAGIADLATGAGKLLGITTGIAYGSTLIAGLLSFAVSTLIFPNFID
                     ASVASQIGDPEAGMLAPIFTIPLEPMVDVTAAIVFAFVMGLGISALRNHGKGETLFNF
                     FQEFQGIVTKTLSTVIIPLLPLYIAGTFANITIAGEVWTILGVFWKVYLVVIPLHFVY
                     MACQFTAAGVFSGKNPVRMLKNQVPGYLTAIGTQSSAATIPVNVGCAEKNGVSKQIRE
                     FVVPLCATIHLSGSMISITGFTTAVLMMNGMPHGIGVLFPYIAMLGIAMVAAPGAPGG
                     AIMSALPFLPMVGIPSDGGLASLMIALYLTQDSFGTACNVSGDNAIAVVVDKINDGMS
                     KKGNKEEQSA"
     gene            complement(37505..38581)
                     /locus_tag="KR250_000030"
     CDS             complement(37505..38581)
                     /locus_tag="KR250_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003415975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBH0066003.1"
                     /translation="MSTNHGANLYSLSSKYGFSKEEFMDFSSNINPFGTSSLAKQYIV
                     NNIDMVSMYPDPDYIDLKTSISNYCKCSIDNIVLGSGATELISSFIHTINPKQALLLS
                     PAYSEYEKELSKINCSIEKYFAKEEDNFHINLENLIKTINAKDYDLVVICNPSNPTGF
                     AFTKVEVREILKNTDSFLMIDETYVEFTDTDTYSCTQLVDDYSNLFVIRGTSKFFSTP
                     GIRLGYGLISNTNVKNEINKNLDLWNINIIASKMGEIMFSDLDFISNTISLMNTERDY
                     LLKELKNIKSLDIYNTKGNFILCKIKTKELTAKSLREQLLPQKIIIRDCCSFEGLDEY
                     FFRVCILKPNENKLLISSLKAIFK"
     gene            38810..39337
                     /gene="hpt"
                     /locus_tag="KR250_000031"
     CDS             38810..39337
                     /gene="hpt"
                     /locus_tag="KR250_000031"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HBH0066004.1"
                     /translation="MYKVTGKMLTEEQIKEKVYELGKKIEEDFKGEDLLVVGILKGAS
                     VFVSDLIRCIDLDVNIDFMSVTSYGNSTESSGTVKILKDLDVDIEGKNVLIVEDIIDS
                     GLTLSNLVAALKTRNPKSLKLCTLLDKPQRRKANIPVDYVGFVIEDKFIVGYGIDYAE
                     KYRNLPYIGIVEDVE"
     gene            39426..40358
                     /locus_tag="KR250_000032"
     CDS             39426..40358
                     /locus_tag="KR250_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HBH0066005.1"
                     /translation="MDIQLNILLFFQNLRNPILNFIFLVFTISTEAPLLILITTIIYW
                     CINKKCGQKILFAIIGNFVVNLGIKEFVKAPRPIGIKGLESLRVSTAGGYSFPSAHTQ
                     TATTFWVSIMTIFKKRYLHLIGAVMVLGVGLSRLYLAVHWPIDVIAGWILGIFFTVVF
                     VKIFDYIDDNKNYILLVVLLIPFIIVAIFLNSPEYFEKFGIIVGFVFGYMVEDRFVKF
                     NTDNNNKKINFSNKNKGSKNIIFSRICRFLVGIVTIGMLYIGIKLFISMLIVTLNISD
                     STMSIIFMNFIKNTVVVFYGIAGAPALFKLLKLN"
     gene            40420..41646
                     /locus_tag="KR250_000033"
     CDS             40420..41646
                     /locus_tag="KR250_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBH0066006.1"
                     /translation="MKKVVIIGGGPAGMIAASTACEKGYDVTLIEKNHKLGKKLAITG
                     KGRCNITNACEIEELIENVPTNGKFLYSAFYTFTNDDVISMFNNLGVKTKTERGKRVF
                     PESDKAFDIVNALERQLKSKKVNILLNSKVEKIISKNNKIEKVILNDKKEIKCDSVVV
                     ATGGLSYPLTGSTGDGYKFAISQGHTIIDTKPSLIGIEVQESFTKDLEKLSLRNVEIR
                     VFNSKQKKVYSDFGELEFTKFGLDGPIIKSASCRMKDTRKENYTILLDLKPALDEEKL
                     DKRVQKDFQKYTNKKFEKALDDLLPKKLIPIIINLSEINPNTVVHQISREQRKNLVHL
                     LKNLKFTVKRYRPIEEAIITSGGVKVNEINSSTMESKLVEGLFFAGEVIDIDAYTGGF
                     NLQIAFSTGYLAGFNC"
     gene            41730..41951
                     /locus_tag="KR250_000034"
     CDS             41730..41951
                     /locus_tag="KR250_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-type small acid-soluble spore
                     protein"
                     /protein_id="HBH0066007.1"
                     /translation="MASNNNNNRTVVPGAKEALNQMKLEIANELGMSNYQQVDKGNLT
                     ARENGYVGGYMTKKLVEMAEQQMAGKSNR"
     gene            42140..42310
                     /locus_tag="KR250_000035"
     CDS             42140..42310
                     /locus_tag="KR250_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0066008.1"
                     /translation="MSSVNFEDLKNKFINSDLDEKIRIYTTTEGLSVEQFKELLKYYP
                     IQHLSKLEKALG"
     gene            complement(42403..42864)
                     /locus_tag="KR250_000036"
     CDS             complement(42403..42864)
                     /locus_tag="KR250_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0066009.1"
                     /translation="MQIEQLDLETRNKIYCYTKKILRKYQKGITSGKLTADKFADNIL
                     SQDFISSILNEKIVNETNFKISYRNYIETLINIQNENLSNLRKKSTKTAKCFNISQIT
                     QLKNLLSNTGYNLLIPYKYLTARDIEGIVTLINTGSIELGNERIYNYISKA"
     gene            43047..43514
                     /locus_tag="KR250_000037"
     CDS             43047..43514
                     /locus_tag="KR250_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0066010.1"
                     /translation="MKMLNGIIKLFYSEIVKTKINNLDSSGYSLRDECKEIRNITLKI
                     DSVFFKLLKLAFGSILLMVAFNLNMVLGIGVFLVGVVYILYKRNMEEQVKEHINNVKN
                     NIDISKITFMDEKGRTGLNALITLLLIGLLTGFNWILVASFVVVFVFTIKNVC"
     gene            43557..45320
                     /locus_tag="KR250_000038"
     CDS             43557..45320
                     /locus_tag="KR250_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HBH0066011.1"
                     /translation="MTNGSYKMKNLVEISAMVTESTDFFNIKDDIIEKMLEVVHPTKA
                     CVNLFYKNDSKYAYLVCSQTLEYVPQIFPINSLKGAKIDFDTYPEYIHEAVNEKKIIY
                     VENVFEDSRAEGERDLAKAEGYIGRIVFPFIINNVVVGFMTCFLKEDDYITEQDIDFI
                     SSVASLLSLSIEITNKNNNVQILIDKLRGSISAINEATKKLYLNKSIDGFLENLSKQA
                     CNITNSKEALIIINSHHNKDAIVSCYNVEQKKKTNLYPMIDMFLKKESLGGYLNNLKP
                     CSKKGINLESYIYYKLQDKNETIGCIVCANSKNYTSDDLNILSILSKQVSVGMQLYEH
                     NEKEVKHKVLENELNILNKQQQLIMDKGKMDCNDEKELYFYHKPARVVGGDFYYATKV
                     DDEHIVYIVADVMGHGMVSNYVVAMIKGAFKVLCNQYNTPREIMTNLNRMLYDEFDKM
                     GVFTTCLIGIIDTKENMITVSNAGHYSPVVVKKDGTIENNLNCKKGIPIGIMEDATYE
                     NNTLSMEDYAMVCMYTDGILEIKNSSKEEYGINRLENFLKENFRLNQQLIVENLKLDL
                     KNFSSKDNYDDDILIVMLKDR"
     gene            45328..45936
                     /locus_tag="KR250_000039"
     CDS             45328..45936
                     /locus_tag="KR250_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBH0066012.1"
                     /translation="MDSNDKKGLKSPEIIIINGSPRINKNCSFIANEVKAMLEECGLS
                     CKIFNIYNMDIEYCTACGFCEKTGYCKFNDDMKPLYKMFDDSKGTIVISPVHFDSISA
                     KLKTVVDRTQAIYASKYVLNKPSIDRSKKRIGMYIAVGGSKSYDSQFKGGQIVMDFFF
                     KSINTKLLHNIYLNNTDDVSYIDNQEFKDIIYKNLKIYIDSI"
     gene            46008..46577
                     /locus_tag="KR250_000040"
     CDS             46008..46577
                     /locus_tag="KR250_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HBH0066013.1"
                     /translation="MKLPIALSNKHVHLSQADIDVLFGAGHELTKFKPLSQPGQYACE
                     EKVDLVGPKGTLKGVRVLGPARAHTQVEISLADGFPLGVKAPIKESGDIAGTPGVKLV
                     GPAGEVEMKEGVIVASRHIHMSNEDAAKFGVTDKQIVKVKTSGPRALVFENVLVRASD
                     SFNLEMHVDMEEGNAAGVKNGDLVELIVD"
     gene            47095..50634
                     /gene="nifJ"
                     /locus_tag="KR250_000041"
     CDS             47095..50634
                     /gene="nifJ"
                     /locus_tag="KR250_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HBH0066014.1"
                     /translation="MAKFMKTLDGNTAAAHVAYAFTDVAAIYPITPSSTMAEVVDEWA
                     SQGRKNIFGQTVNVVEMQSEAGASGTFHGSLQGGALTSTFTASQGLLLMIPNMYKVAG
                     ELLPGVFHVSARALASQALSIFGDHQDVMAARQTGCVLLASGSVQEVADIAPVAHLAA
                     IEGRLPFIHFFDGFRTSHEIQKVELLENEDYASLLNFEAVQAFRDNALSPNHPVARGT
                     AQNPDIYFQTREASNKYYQNIVGIVEKYMEKMSNLTGRKHSLFDYYGAEDAKYVLIAM
                     GSVTETIEETIDYLNAKGEKYGLVKVHLYRPFSMKHFLDAMPSTVERICVLDRTKEPG
                     STGEPLYLDVRDVFYGKENAPMIIGGRYGLGSKDTTPSDIKTVFDNLVSEQPKNGFTV
                     GIVDDVTNTSLTPSEPIKIASKGTIRCKFWGLGSDGTVGANKQAIKIIGDHTEKYAQA
                     YFDYDSKKSGGITMSHLRFGDTPIRATYLIDEADYIACHKQSYVYQYDLLKGLKKGGT
                     FVLNTIWDQAGLEEHLPAHMKQYIAKNDIKFYTVNAVKLGQEIGLGNRINMIMQSAFF
                     KLAEIIPEEDAVKYLKDSIVKAYGKKGEKIVNMNYAAVDAGINALVKVEVPASWENAV
                     DADKGEVKEPEFIKNILRPMTAQEGNNLPVSTFNGIEDGTFPCGTAAYEKRGIAVDVP
                     EWIIDNCIQCNQCSFICPHSCIRPVLVTEEELANAPEGFEAKKALGKGLEGLKYRMQL
                     SPLDCTGCGNCADICPAKEKALVMKPIDTQLDTELDNWAFAVNPDEVAPKGDVMPANT
                     VKGSQFRQPLMEFSGACAGCGETAYIKVVTQLYGDRMMIANATGCSSIWGASAPSIPY
                     TCNHEGKGPSWANSLFEDNAEYGFGMYTAVKQIRNKIVDAMTELVSMDICEDAKAVFT
                     EWLDSRNDGEASKVASAKVVELLEKPACDCTDEKAKELVKAIKDRKDYLVKRSQWILG
                     GDGWAYDIGYGGLDHVLASGEDVNVLVFDTEVYSNTGGQASKATPAAAMAKFAASGKK
                     SRKKDLGMMAMTYGNVYVAQVAMGADKNQFIKAVIEAEKHDGPSLIIAYAPCINHGLK
                     EGMGRTVANEAQAVACGYWHLYRFNPEVKAEGKNPFTLDSKEPTASFKDFILAQVRYS
                     AIAKQFPEEADGLFAKAEADAKERYEGYKKLAE"
     gene            complement(51026..51886)
                     /locus_tag="KR250_000042"
     CDS             complement(51026..51886)
                     /locus_tag="KR250_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein"
                     /protein_id="HBH0066015.1"
                     /translation="MMRRNIPTKLKTILALGVLTLATASSAVTASASSLENNVNISNK
                     SLVYSNTKYKFSAKPNCSVKPELKPDKDNSCKDKNHNDKNCNNTNKPEDNNNSDSTNK
                     PGNNKPESNKPEDNNNSGSTNKPDNNKPESNKPEDNNNSDSTNKPDDNNNSGSTSESF
                     SAYQKEVVDLVNIERAKAGLNPLTLDSSISNVATKKSQDMIDNNYFSHNSPTYGSPFD
                     MLKKFGISYKTAGENIAMGQKTPKEVVNAWMNSEGHRKNIMNPNFSKIGVGVAQKSGG
                     SIYWTQIFVG"
     gene            complement(52382..53716)
                     /locus_tag="KR250_000043"
     CDS             complement(52382..53716)
                     /locus_tag="KR250_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HBH0066016.1"
                     /translation="MSIIAPIGIIIGIIAIIYFSVKEIHITIAAPIATLIVVILNKMD
                     VVTSMLGADKNNYMGALGNYIMSYFAIFLLGSILAKFMEESGATVSIADFILNKFGSN
                     HPYRVLVAIFIVSFILTYGGISLFVVMFAVIPLARTLFKKLDISWNLIQIPLWLGIAT
                     ITMTIIPGTPAIQNVIPIQYLNTSLTAATIPSIVGSIGCATFGLVYMKYALNKSIRKG
                     ETYATYTSELEEESLDRELPGFFASIFPLLALVIIAITGSTFGSEFMKKNVIYIALIV
                     AILLSAFLFHKFLSSKIQTLSIGASGSIGPIFATSSAVAFGAVIMAAPGFDVFSKLIM
                     SIPGSPLISLTVLTSTMSAITGSSSGALGIVMPNFADYYLSTGLNPELIHRVATIASN
                     ILTIVPQSGVFLTFLSLSKLTHKTGFKETFIVVAVGSLIAEIIVITLGLLMY"
     gene            complement(53778..54554)
                     /locus_tag="KR250_000044"
     CDS             complement(53778..54554)
                     /locus_tag="KR250_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HBH0066017.1"
                     /translation="MVKDKVVFVTGAASGIGKQIAESFLKNGAKVMFSDINQEALNNT
                     ITELQKKGYDCMSVKCDVTKEEEINHAIDKTVEKYGRLDILINNAGLQHVSMIEDFPT
                     DKFEFMIKIMLTAPFIATKKAFPIMKKQGFGRIINMASINGVIGFAGKAAYNSAKHGL
                     IGLTKVAALEAADSGITVNALCPGYVDTPLVRGQFNDLSKTRNIPLENVLEEVLYPLV
                     PQKRLLDVQEIADYAMFLASDNAKGITGQACLLDGGYTAQ"
     gene            complement(54659..55324)
                     /locus_tag="KR250_000045"
     CDS             complement(54659..55324)
                     /locus_tag="KR250_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HBH0066018.1"
                     /translation="MDKLEMQEYIANRVSKELKDGAVVNLGIGLPTKVANYIPDNVNV
                     ILQSENGFLGLGSAEDGNSSDETIVNAGGQPVTILPGGCFFDSATSFGIIRGGHVDIT
                     VLGALQVDKYGNIANYMIPGKMVPGMGGAMDLVTGAKKVIVAMEHTSKGSAKILNNCT
                     LPLTATNAVDLIVTEMGVMEVTSDGILLKEINPAFTLDDVISATEAPLILSDSLNGKI
                     SVV"
     gene            complement(55324..55980)
                     /locus_tag="KR250_000046"
     CDS             complement(55324..55980)
                     /locus_tag="KR250_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HBH0066019.1"
                     /translation="MNKIVSIDEALSHVKDGDTIMVGGFMANGSPENLIDYLCSKNIK
                     DLTLICNDTGFIDKGVGKMVVNKQFKKIIASHVGLNKETGRQMNNNETDVELVPQGTL
                     AEQIRAAGYGLGGILTPTGIGTLVEEGKQKIVVDSKEYLLEKPIYADVALLFASKVDK
                     AGNLVYKGSMNNFNNLMASAAKITIVEADEIVEIGDIDPNEVNTPGIFVNYIIEGGNL
                     "
     gene            complement(55989..57167)
                     /locus_tag="KR250_000047"
     CDS             complement(55989..57167)
                     /locus_tag="KR250_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000786547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HBH0066020.1"
                     /translation="MKDVVIVSAVRTPIGSFGGVFKNTSAVQLGTITVKEAISRVGLN
                     LSEIDEVIIGNVLQTGLGQNVARQIAINAGIPNSVPSYTVNKLCGSGLKSVQLAAQSI
                     TSGENDVVIAGGTENMSQAPYIVPTARFGSKMGDITMVDSMLTDGLIDAFNQYHMGIT
                     AENIATKFEFTREMQDKLALESQSKAENAIKNNRFKEEIVPVDVLIRRGKIETIDKDE
                     YPKLGMTFEGLAKLKPAFKKDGTVTAGNASGINDGAAMLILMSQQKADELGIRPLAKI
                     KSYASAGVEPEVMGTGPIPATRKALKKAGLSINDIDLIEANEAFAAQALAVKNELQID
                     SSKLNVNGGAIALGHPIGASGARILVTLIYEMQKRKVETGLATLCIGGGQGISMVVSR
                     "
     gene            57522..58424
                     /locus_tag="KR250_000048"
     CDS             57522..58424
                     /locus_tag="KR250_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBH0066021.1"
                     /translation="MDIKQLKYFVEIVKSGFNLSIASKTLHISQPALSQIIKNFEESE
                     DVYLFERYKGRLNGLTPAGERFYVNAEHIINEYKNMMEDLREDSVQFKGKVRIGIPPL
                     ILGIAFSDVVAQLVANNPDIEFDIVEKGAYDLNRMLILQELDYAVLLHPHKIDKSVVT
                     EHVLQEDELTAFLNINHPLAKKDKIDWNDLNEQLLAIFNPTFMIHHKLMQKFTDENVK
                     LKRYIMSGSWDFLLLSTTNSDFITILPSPVHDFFENSKIIEKPFNHPITWKVILCRPK
                     KERYSHVDQHVFKFIIDFFSKKNS"
     gene            complement(58640..59452)
                     /locus_tag="KR250_000049"
     CDS             complement(58640..59452)
                     /locus_tag="KR250_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0066022.1"
                     /translation="MFTSILKKLKKDKSFAFACITLSIIIILIIASFILNIDSNAIDT
                     TSKLQEPSLKHIFGTDELGRDYFSRALYGGRISLSVGILSMLISVVFGTAVGVISGYI
                     GGKLDQFLMRLIDMLMSIPSFLVLIVLNTYLTPKVSTIILIIGFLSWMEVSRIVRGET
                     LKIKENEYCLAAKATGIKTRNILLRHIVLNLKETIVVAGSLNIANAILTESALSFLGL
                     GIQLPLASWGNMLQDAQRHIFDKLYLAVFPGLLIFLTVMSLTIISKKFSSNT"
     gene            complement(59455..60414)
                     /locus_tag="KR250_000050"
     CDS             complement(59455..60414)
                     /locus_tag="KR250_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0066023.1"
                     /translation="MLKLILNRVKVLIPMLILISILSFGLLELAPGDPADAYINPLMS
                     SQDIENIRVNMGLDKPVYIRYLNWLKNTLSGNLGISYINHMPVTEQIMEKMGNTFILM
                     GTSLIFSILVAIPLGIFLAVNKNSITSKVSSVFNYIGVSIPSFWIGMILISIFSVKLN
                     IFPSGGMHTIGNDSIGDLVKHLVLPVITLGLYNTAIFTNYVEASVNEQLKKQYVITAR
                     AKGLSEKVILFKHVLKNSLTSLVTILGMSIQKLVTGAFVTEVVFSWPGMGRLMIDSIF
                     SRDYTVIMAITMLSALFLILGNLVADILYLLIDPKIKSSKGGF"
     gene            complement(60520..62079)
                     /locus_tag="KR250_000051"
     CDS             complement(60520..62079)
                     /locus_tag="KR250_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBH0066024.1"
                     /translation="MKFKKLASLILVSSLMLTFTACASNDKDKSSGSKSGGTVVIPMA
                     SDPDIINGAFANVKEDSLASNIVYAPLYTYEKGNLVNYLAEKVDFKDSKELTIKLKSN
                     LKWHDGKPITAEDVLFTFNTVLDEKQNSPSRQYLLVGEKPVKVEKIDDLTVKITLPTA
                     SESFLYGISKISPIPKHVFEGESNIAKSEKNNNPVGSGAFKFKEWKKGESIVFEKNAD
                     YFGGEPKADSIALKIIPNEASQEAALNNGEISLMKTSAEGYEKAKSNSNLQTYTYSEE
                     RLNYIVFNQNISNMANKEVRQALSYALNRNEMIESAYGKEGSVPAKSILVPEADFYTE
                     EGVEGYDQDTNKAKDLLDKSGVKIDKLKIGYNTGRFGHKNYALVAQQELKKIGIEAEI
                     VPYESKAFFNILFSNSTECDMYVNGYAWGLEPNPYRGMFETGQYCNQTKYSNAEIDAL
                     WEKGFTELNKEKREEIYKQIQQDISKDAPIYTIDYEQNLMAAQKNLKGIKDAKPSPAI
                     LFEDWSKLYVE"
     gene            62298..63275
                     /locus_tag="KR250_000052"
     CDS             62298..63275
                     /locus_tag="KR250_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0066025.1"
                     /translation="MSKALIEVKDLKVYFHTDKGIVKSVNEVSFNINEGETIGIVGES
                     GCGKSVTAMSLMKLLPTSKIEGGEIIFRGKDILKMNEDELMGIRGNEISMIFQEPMTS
                     LNPAFTVGSQIIEGIMIHQDLSKEEAKKKVIDMIKLVEIPRAEEIYNSYPHELSGGMR
                     QRIMIAMALSCNPKLLIADEPTTALDVTIQAQILDIMKNIKEKLNTSIMMITHDLGVV
                     AEMCDKVLVMYAGKIIEVAEVVELFKNPKHPYTIGLLKSKPALGKNKDKRLYSIPGQV
                     PNPIGMPDSCYFSDRCEKVCDKCRTQIPPLIELNSGHSIACWLYKKEEI"
     gene            63276..64247
                     /locus_tag="KR250_000053"
     CDS             63276..64247
                     /locus_tag="KR250_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HBH0066026.1"
                     /translation="MKKEESLLKVEGLKKYFEVKSGLFDKKIKSVKAVDGISFDLKKG
                     ETLAIVGESGCGKSTAGRTILRLIEKTEGKVEFNGVDIYKLEKEELRKLRPKMQMIFQ
                     DPYSSLNPRLNVEQIISEAVIEHNLVPKNEIKDNILNVVESCGLSKYHLNRKPGEFSG
                     GQRQRIGIARALALNPEFIVCDEPVSALDVSIQSQIINLLMDLQDKYKLSYLFISHDL
                     SVVNHIAHRVCVMYLGSIIEIATKGELYLHSQHPYTKALMSAVPIQDPTIKKNRIILK
                     GDIPSPTNPPSGCKFHTRCPYCKEICKKEIPVLKEISRNHFVACHLV"
     gene            64399..66441
                     /locus_tag="KR250_000054"
     CDS             64399..66441
                     /locus_tag="KR250_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HBH0066027.1"
                     /translation="MSILVTLLGLLVCTTISTVFSKKWSNIPLAIYQIVLGIILSILP
                     FKFSFSFNPEIFVICIIAPLLFSEGQNVSRKELLELRKPILLLAFGLVLITVFAGGIF
                     IHFLIPGMPLSVSFALAAVISPTDLVAVKSITQGLNFPKNIMSILEGESLLNDAAGVV
                     AFKVAVLATVTGVFSIEEAGIQFMITAFGGIIVGSILGYIIIKIRLSLHKWNLEEIPM
                     VIVIQIMTPLFVYFVAEEIGVSGILAVVMAGIAHGIEKEHLQNTTTKLRIISDNTWYV
                     LEYVLNGFVFTLLGFLLPSIYTGLSSKNENMALELTFISTLIVLILFVIRFIWVYLWH
                     NSFIKPKKNPLNNFFVGFLGFKDEEVIKESISKCKYSLIVATCGVHGTFTLATALSIP
                     FYLADKDVFPMRDTVLFISSEVILISLVLATILLPRLLKNNVQESEPLLSDKEAYKLI
                     LKEAILKLSNEKAQEVQPVIHDLNEQLVDSEKGILNTPDNKLISMMIAEADQQGLLAV
                     LKLLEDGKISDRAFKLYRFYITKSRKSVQKSIFKIVKLKISMWIINRKVKKEVKEKIT
                     KIVSENKEIIKEFHHAYALSTKTAISFINKNTDEDNRHEALIAIRFYNRYLNWITRKI
                     QNDDKFEARVEYYRVMAIQSQRDSVQQLVETKQISREVAKELLENILYDEMMMIES"
     gene            66702..67544
                     /locus_tag="KR250_000055"
     CDS             66702..67544
                     /locus_tag="KR250_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBH0066028.1"
                     /translation="MIIQQIYNNNVVLVLDENIKKELILTGCGIGFQKKKGQEIDKSK
                     IERTFVIQDESFLDKISKLASQVDEKFFEVSTEIIAYAEENLNTKLYEYIYVALTDHI
                     AFAIKRYHENITIKNDLLHEIKRIHKKEYEIGKWAVDYINKEFDVKFPVDEAGFIAMH
                     IVNSNYKGSSKESLLITKIVKDILNIIRYYYRVEFKEDDINYDRLLTHLKFFAKRLVK
                     KEKINDTNNEIIDIIKVKYEKDYDCAYKIKTHVEKNYDYYVSQDELLYLTLHIKRVIS
                     VLNL"
     gene            67847..69919
                     /locus_tag="KR250_000056"
     CDS             67847..69919
                     /locus_tag="KR250_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBH0066029.1"
                     /translation="MKKVFGVLQKVGKSLMLPVALLPAAGILLGVSNALAGDALLAHF
                     PALANPTFQLIISIMESSGQIIFNNLPLIFAVGVAVGLTEGEGVAALAAIVGFLILNT
                     SMGVMAGVTEDMVDNSMYANIVGIPTIQTGVFGGIIIGIVAAYLYKKFYKIELPQYLG
                     FFAGKRFVPIVTSLVAIAIGIILSFIWPPIQEGLLSFSKNVIEANQTLAAFMEGSLER
                     LLIPFGLHHVFHNPFWYQFGEYVNKAGELVMGDQKIFFAQLKDGVNFTAGTFMTGAFP
                     FMMFGLPAAALAMVHEAKPEKKKFVAGIMASAALTSFLTGITEPIEFAFLFVAPVLFA
                     IHCLFSGLSYAIMQLLGVKIGFTFSGGLIDFLLFGVLPNRTPWYLAILVGIGFAVLYY
                     SVFRFMIRKFNLKTPGREDESETNAEVIEMSKNELASEILIALGNKDNLKSLDACITR
                     LRLVVNDINKVDKEKLKSLGAAGVMVIGNNVQAIFGPKSDSIKSDIKDIIEGKTKVES
                     TVTKNVDKNKVKKNMEVANMENALVEKIDSLTNGEILDIEEVPDSVFSSKLMGDGFAI
                     KSSDGLIYSPVDGTIGVIFPTKHAIIIKSQKSGVEILIHLGIETVNLEGNGFEVFVNV
                     GDEVKAGEKLVKMDLEYIEKNGLSTISPVVFTNLEQNQKLSIKKGVSTAKEENRVSIL
                     RDDIEKIG"
     gene            complement(70132..71001)
                     /locus_tag="KR250_000057"
     CDS             complement(70132..71001)
                     /locus_tag="KR250_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH0066030.1"
                     /translation="MDNKIGYLNDNFKIFNIRDKKDIQFEYHNHDFNKIIIFINGNVT
                     YFVDGIPYKLKPWDILFISNNEIHKPVIDSNVFYERIAIWISPEFMKKNSDASSDLEK
                     CFKIATENKCNRLRLNIHDIESIKSFIEQINISESNTEFGNEILRNALFIQLMIFLNR
                     IFLKNENMESTEDVFYDETIKKVLNYINSNIDDKLSIDSISSEFFISKYYLMRKFKSH
                     TGTSIHNYIVQKRLILAKSLITNGHLMSEVCNKCGFNDYSSFVRAFKKNYGVSPKNYI
                     NNKNPLDITLLDE"
     gene            71165..72619
                     /locus_tag="KR250_000058"
     CDS             71165..72619
                     /locus_tag="KR250_000058"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HBH0066031.1"
                     /translation="MKLNDIIQGLDIINIKGELNIDINNVQYDSRKVTKGTLFICIKG
                     FVSDGHKYIKDAIEKGASAFLVEEDVEIKGCTFIKVKDTRKDMAKVADNFYNHPSQKF
                     NVIGVTGTNGKTSITTILNEILTLNKNKVGLIGTIKIFDGEKDIVSNSTTPESIDLQY
                     HFNNMLDNGCDYCAMEVSSHSLALNRVDETDFKLGIFTNLTPDHLDFHKDLEDYRKAK
                     EKLFFKTTMANIINIDDEGGKKIYENIKGINVPCYTYGVDTKADFMARDIKSDSDGVS
                     YRLITPSYEEVIFIPVPGMFTVYNTLAVIAACYVLGIPKPIYKEGLRLSNGVSGRFET
                     VPNDKGISVIVDYAHTPDALENVLKTTQQFAEGKIISVFGCGGDRDTEKRPLMGAIGQ
                     KYSDLCIITSDNPRTEEPEAIIKDILEGIDKKKENYHVVVDREQAIAEAISMAKKDDV
                     VIITGKGHETYQIIGKVKHHFDDKEVANECLSKM"
     gene            72890..75169
                     /locus_tag="KR250_000059"
     CDS             72890..75169
                     /locus_tag="KR250_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBH0066032.1"
                     /translation="MLSDAKNNRYGLSIIAVISILVITISCLIYGSRLNKTLGEETNQ
                     YLSEIANQSVNVLKKQINGDIKLLESISIFIEGEESFEVDNILSILKRQAINSSFKRM
                     GVILPNGTAYTTDGYIEDFSKRDYFLKSMQGEVVITGKLKDVIEDDKNTNSNINVYSV
                     PIYKDNEVKGVIFATHSTKLYEKILSVPTFNGKGYSYITRSNGDIVLNPSSKNANSTK
                     ENLFSYIGRSDSISKSSINNMKSNIKNFKSGSLSYSMFGKGYYLSYAPIGINDWYLFS
                     EVPKTAISEKSYAIIKLTLSACVVLIIIFTALTIYILFIHKKSKEKLEEFAFKDNVTG
                     IGNSNKFNLEGGKFLSSHEKKNLVLIYFDIDKFKLVNDRFGYEEGDRVLKEIAEIIKN
                     IFKEQSVFSRISNDNFAIIFEKKNNRESIIEICELIRKKLSMIKTSLGVELNLIPSIG
                     VYFVEEGETNISTCLDKAMIAKTTVKRKYLKVYEIYAENLKETLIEERDIEQEMHDAL
                     KNEQFKVYLQPKVDLSTTKIVGAEALVRWQHPKKGLISPGVFIPIFEKNGFITELDMF
                     VFTQVCKNFKRWENENFPTFPISINLSRVHLENPGFISELERITKEYEIEPNLIEIEL
                     TESAIFDNTKILFKIMQTLKSVGFKISMDDFGSGYSSLNMLKDMPIDVLKLDRQFFIT
                     VGNAKKSQIVVSSIVQMAKQLDIKVVSEGVETVEQAEFLRFIGCDMAQGFLFARPMPI
                     EEYEKLILDRYSLNRKETN"
     gene            complement(75648..76745)
                     /gene="ychF"
                     /locus_tag="KR250_000060"
     CDS             complement(75648..76745)
                     /gene="ychF"
                     /locus_tag="KR250_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HBH0066033.1"
                     /translation="MKLGIVGLPNVGKSTLFNAITQAGAESANYPFCTIDPNVGVVSV
                     PDERLNKLQELYNSEKIVPTAIEFCDIAGLVRGASKGEGLGNKFLSHIREVDAIVHVV
                     RCFEDENVVHVDGSVDPLRDIETINLELIFSDIEILERRINKTQKAAKADKTLGSELD
                     LLKSIMSTLEESKCVRTMEFTEDEQTFVNSLDLLTSKPVIYASNVSEDDLADNGENNK
                     YVQQVKAFAETEDAEVVVVCAQIEAEISELDSAEEKKEFLETLGLEQSGLDKLIKSSY
                     ALLGLISFLTAGPKEVRAWTIKVGSKAPQAGGKIHSDIERGFIRAETIAFNDLVEHGT
                     MAAAKEKGLVKLEGKEYIVKDGDVILFRFNV"
     gene            77061..78326
                     /locus_tag="KR250_000061"
     CDS             77061..78326
                     /locus_tag="KR250_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBH0066034.1"
                     /translation="MVNTKKIINLGNNINLTLIKTEKFKSNLISLYVQRLLDEKETTK
                     NALIPSIIASGSSKYPSARAISNKLDDLYGSSMGADAVKRGERQVLSFKVINISEKYL
                     DESIFEEVVEFFNEVINNTLVVDGGFKEEYLNIEKENLREKIQSIINDKKEYAQDKCI
                     EAMCKGERYSVSEFGYEDEIDSITSRELYEHYKNILKTSPIDIVVEGNFDEDKVVDII
                     SKNLKFEREEIINIPRADFIKNVDEVKVIDEQMEITQGKLVMGYRANVDYADIDKYYA
                     LVVGSNVLGGGPHSKLFVNVREKESLCYYIFSSIEKYKSIMFISSGIETKDYDKAVEL
                     IKKQVESVKAGDISDEELENSKLALVNSMKSITDNIGGMSDFVFSQSMAKTNSEVQDI
                     ISSIEKVTKKDIVEAIKNIELDTVYFLRN"
     gene            78341..79627
                     /locus_tag="KR250_000062"
     CDS             78341..79627
                     /locus_tag="KR250_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBH0066035.1"
                     /translation="MEKIVNDILKEEVYYEKLQNGLDVYFMPKRGFMKKYAILATNYG
                     SNDLEFVPIGEDKKIRVNEGIAHFLEHKMFEQPDGGDAFDKFSKLGVNANAFTNFTMT
                     AYLFSATENFYESLEHLIDYVQTPYFTDENVEKEKGIIAQEIKMYNDDPDWNVYFNCL
                     KAMYVNYPARIDIAGTVDSIYKITKEELYKCYNTFYNPGNMALFVVGDLDVEKVIDVT
                     KKSNNYKVDKLSKSIERFYPEEPEGVKEKEVIEKFPISMPMFNIGFKDSNVGLKGKEL
                     LRKEIVTDILVGMLFKKGSKLYEDLYMQGLINENFGAGFSSQVDYAFSIIAGDSKEPK
                     KVKEIILDYIEKSKKEGLSKEEFERTKKKKIGSFIKCFDSINFIGNSFISYVFKDINL
                     LEYLDVIKDITFEEVEERLKEHFKEEYCVISIVEPK"
     gene            80021..80767
                     /locus_tag="KR250_000063"
     CDS             80021..80767
                     /locus_tag="KR250_000063"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HBH0066036.1"
                     /translation="MDRVAFTLFGIDIMWYGILMACGMILGTLIAIKEAKRVGIKEDD
                     VLNIAIIAIPVGLICARIYYVVFNWSYYAQNMSQIFNFRGGGLAIHGGLIGGILAGYI
                     YTKIKNINFLKMADTVILGMPLAQAIGRWGNFINGEAHGGATNLPWGIMVDGVKVHPT
                     FLYESIWDFGIFIVLLLFRKNKKYEGQVIVTYITLYSIGRFFIEGLRTDSLMLGPLRM
                     AQVISLIGVIGGIIAHVYLSKKNKNNISEE"
     gene            80797..81732
                     /gene="rsmH"
                     /locus_tag="KR250_000064"
     CDS             80797..81732
                     /gene="rsmH"
                     /locus_tag="KR250_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HBH0066037.1"
                     /translation="MEFHHVSVLLNECIENLNIKPDGVYVDCTMGGAGHSKEIVKKLS
                     DKGLFIGFDQDKNAISTAKERLSEYESRVKFVHSNFENIKEELEKIGVYKIDGVLADL
                     GVSSHQLDEADRGFSYMQDAPLDMRMDVRCEFSAYDVVNTYTEDELTKIIKDYGEDNW
                     AKRIAKFIVEERANKPIETTGELVDVIKKAIPKKARIDGPHPAKRTFQAIRIEVNNEL
                     GVITKMINDASSIMNEGGRICIITFHSLEDRIVKNAFKHLASDCICPQHLPICQCDKE
                     SEVKIITRKPILPSEEEIEVNPRSRSAKLRVAEKI"
     gene            82218..82565
                     /locus_tag="KR250_000065"
     CDS             82218..82565
                     /locus_tag="KR250_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0066038.1"
                     /translation="MKKNYKIHNLNEYKNKKKNNYKKRNKKKIGAKCILLIATFSVII
                     LNLCGYSKISEMKYDIYYLQKDLRKKEIVLEELKAKETEKTSIKEIELKAKELLNMDY
                     PKDGQIKYIDVDN"
     gene            82577..84556
                     /locus_tag="KR250_000066"
     CDS             82577..84556
                     /locus_tag="KR250_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein D"
                     /protein_id="HBH0066039.1"
                     /translation="MRKVKRISKKRLVLVLILACALFFCLVIRTGYLQLMKGNWLSTK
                     ALEQQTRDIPIEPKRGTIYDRNMKELAVSVTKYTVWCKPVEVEDKKEAAEKVAEILDE
                     DYKDIYALISKKNMALVKVKRWIDDDKASQIRDAKLSGIWVAEDNQRYYPYGNFAPYV
                     LGHTSSDATGISGVEMQYDKKLKGKPGKLIVSTDASGREIPQGMEKYYEPVQGNGLVL
                     SIDEVIQHYTEKAVQKAYELNNAKKVTAIAMNPKTGDILALASKPDYDPNDSRTPIYP
                     YYQEELEKYNDKDKIKGYYQMWRNPAVSDTYEPGSTFKLITSSSALEEGVIKDGEKFT
                     CTGSVTVGGRKIKCWRHYRPHGTQEFKQAVQNSCNPVFVELGSRLGVGKMYDYIESFG
                     LMDKTGIDLPGEAKGILYNEKNVGPVELATISFGQSISVTPIQLITAISSIANGGDLM
                     QPRVVKSYTDNKGNITETVKPKKVRSVISKETSKKMLEIAESVVTEGGGKIAYIPGYR
                     LGGKTGTAQKVIDGKYAPGKYICSFVGIAPCDDPQIVVLAIVDEPTGVSAFGSTTAGP
                     IVKEIMNDSLKYLGVKPVYKEEEKAEYEKKQVKVPDVRNLKIGDAVKALEDAKLKPDL
                     DADIELPEDTKVKDIFPKPGVKVNEDSSITLYFEN"
     gene            84723..86096
                     /locus_tag="KR250_000067"
     CDS             84723..86096
                     /locus_tag="KR250_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HBH0066040.1"
                     /translation="MECLTIKELVLATNGKLIYGDYNDCVSDIVIDSREANAQNAFVA
                     IVGENLDGHTFMKSAYDSGCKTFIKNESNGIKLESSDINLIEVKDTSLALGDISKYYK
                     EKFDIPFIGVTGSVGKTTTRDMIYSAISAKLNILKNEKNLNNHFGVPLTLFNLNKEHE
                     CAVIEMGMSGFNEIKYLVDIVNPKIAVISNIGLSHVEKLGSQEGILKAKMEITSNFDE
                     TNTLIVNGDDKFLSTLKEKEHVYKLKTFGFNKNNDVYCESYTMEEDSLTFICVINGKK
                     EEIFIPTIGEHNIYNAMSAILVGLCLNISLDYIKKGLKNFKGTKMRLDIIKNEKLTII
                     NDSYNASPDSMDAALKILGRYKGRRVAILGDILEMGEISEYGHRLVGKSSMNNTDIII
                     TIGENSVFIGEEAKQLGFNSANIYHFENREDVFNKLNELVKTGDTILVKGSRGMRLEK
                     IVEYLNK"
     gene            86137..87105
                     /locus_tag="KR250_000068"
     CDS             86137..87105
                     /locus_tag="KR250_000068"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HBH0066041.1"
                     /translation="MMLGITELTYTALIAFLIVIIIGPIFIPMLRKFKFGQTVRDDGP
                     QTHLAKNGTPTMGGIIMIVAILITGLTRVKVSHDMAVGLICIAGFGFIGFLDDFIKIK
                     LKRSLGLKAYQKIILQVALSFYVAFYQYTSSSSASQLMIPFTDFVINVGILYIPIMMF
                     IIVAIVNAVNLTDGLDGLASGVTLIVSVFFMLFASSIAGNTEVAVLAAATVGACLGFL
                     GFNSYPARVFMGDTGSMALGGAVVAFSVLTNSVLIIPIIGGIYFAEALSVLIQVGYFK
                     ATRKRFFKMAPIHHHFEQCGWPETRVVFIFWIITVVLAWISIIAVF"
     gene            87124..88479
                     /locus_tag="KR250_000069"
     CDS             87124..88479
                     /locus_tag="KR250_000069"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HBH0066042.1"
                     /translation="MDLKGKKVLLVGLAKTGISTIKHLDKLGASVIVNDIKDENKLRN
                     ILDELKSINDIKYILGHHPEDVDDIDMVVVSPGVPLDLPFILKLKNSGKYIIGEVELA
                     FKLSNNPIFIGITGTNGKTTTTSLVGEIFSRAKRDTYVVGNIGNPVIDTIETSSEESV
                     LVTELSSFQLESIDEFRPKVSAILNITEDHLNRHHTMEKYIEAKANIFMNQTVEDFCV
                     LNYDDEIVKSLADKCNAKVIYFSRTKKVNGGVYLENNDIIIDIDDKIKFLNKDDVSLP
                     GGHNLENCMAAIAIAYVCKIDLEVIKDVLMTFKGVEHRQEFVRNLDNVIYVNDSKGTN
                     PDSTIKAIQSYDRPIILIAGGMDKGSNFDELLETAKSYVKSLVLLGETASNIENCAKN
                     KGFNDIHIVKDMEEAVKTSYEISKSGDIVLLSPACASWDMYESFEVRGKDFKDNVNNL
                     K"
     gene            88531..89661
                     /gene="spoVE"
                     /locus_tag="KR250_000070"
     CDS             88531..89661
                     /gene="spoVE"
                     /locus_tag="KR250_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein E"
                     /protein_id="HBH0066043.1"
                     /translation="MPKENLKKQIDIRMKTEFDGVVFYTTMLLVFVGIVMVFSASFIQ
                     SSFKHNDAYYFLKKNVIYAVLGFIVMIITSRIDYSFWKKNATAIGAIAVVLLLLVLTP
                     LGIEANGAKRWLGIGALTFQPAEIAKFATIILTAKLIEKNYDKIKSLTKGVVPLLVVP
                     GIFFALIILQPNLSTAGTVILVTFVMIFVAGMDMKIVFAMIGSGAALFAALVIAEPYR
                     LSRVTSFLDPFQDPLGKGYQVIQGLYALGSGGLFGLGLGKSKQKYFYIPEPQNDFIFA
                     IIGEELGLIGCIIVIMLFVVLVYRCVRIALKTSNVFACMVVIGIGAQIGIQAALNIAV
                     ATSSMPATGVALPFISYGGTSLTIFMGAVGIVLNISKHVKIN"
     gene            89679..90905
                     /gene="murG"
                     /locus_tag="KR250_000071"
     CDS             89679..90905
                     /gene="murG"
                     /locus_tag="KR250_000071"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HBH0066044.1"
                     /translation="MKVLLSGGGTGGHVYPAIAIANKIRDEHPDAEIIFVGTEKGIES
                     EIVPKYGFELKTVTVQGFKRKIDFDNVKRVFKLFKGLEQSRKIVKKFKPDIVIGTGGY
                     VSGPVLFNASMGKIPAIIHEQNSFPGVTNKILSKTVTKVLTSFEDSHKRFPEAAEDKL
                     VFTGNPVRKEILLSRKNIARKNLSISDEKRMVLCYGGSGGSRKINDAMRLVIKNMVNE
                     DIAFIFATGKSYYDEFMGSISDINLKPYQKVVPYLEDMANALAASDLVIGSAGAISLA
                     EITALGKPSIIIPKAYTAENHQEYNAKSIEKQGAGIAILEKNLTPESLNTAVFKLLGD
                     RELLVDMANASKTIGKPEAIDLIYDEIMKVYNSTQKSTSKKTKKEKVIKEVKEIKKET
                     TPSIEGQAKVIGIKKR"
     gene            91175..91915
                     /locus_tag="KR250_000072"
     CDS             91175..91915
                     /locus_tag="KR250_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HBH0066045.1"
                     /translation="MKKRRKLNTNNVMIVLVFILMLSFCICIIVKSDLFDVKKIDVIG
                     NKRVTKSNIMKELNVNLNENIFAYNFKDMKNKLIKNPYIENVEIKRKLPNKIIISLKE
                     KEIFAVLKDEDNYCYIDKKGNLLEELRGSNESKKDLIVDVDYSIDDNKSIKFKNYKTK
                     ENVFKTLNYLKEEGIYRKINYVNLKKESNIEMLTRSNIKILLSNDDNLDYNISRVSKI
                     LIDLQNKNTNGGTINLNYGKLAVYSPEG"
     gene            91938..92636
                     /locus_tag="KR250_000073"
     CDS             91938..92636
                     /locus_tag="KR250_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HBH0066046.1"
                     /translation="MKKRFINKSIIIGSIVLGVLVSLQLKIINIENRGMTTFKRGEQL
                     TEELKSLKKEKENLQSEVNSVKKDIEIYRGKNNEGEQDILKSEIEKYETLAGYTDVEG
                     TGIKIEINSTKDLGNSDKNDSILYNYDLLLSMINKLNSAQASAISINDQRIVSNTYMY
                     LKEDKLYMNDTVIKEPIIIKAIGDSDTLASALNIKYGIVWEIETYYNAKVKVTKEDNI
                     EINGHSDINKAEGK"
     gene            92641..93315
                     /locus_tag="KR250_000074"
     CDS             92641..93315
                     /locus_tag="KR250_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HBH0066047.1"
                     /translation="MKKKIVILGAFILGVFISTYIKSLDPNRVYITLGQTESIESEVE
                     NTKKEIKNLKETLKKNKKTLNEYKEAKKNGNESIQTLMQKELEEVKELSGYSTVTGPG
                     ITITMRDSERELKDGQNPNDLIIHDIDILRVLNDLKKAGARAISINGERVLATSKIKC
                     SGATITVNDTTYGQPFIIKAIGDIDLLSAAINSPESYAKSLKDIYGINIKVQENNGNK
                     VNLFAK"
     gene            93414..93767
                     /locus_tag="KR250_000075"
     CDS             93414..93767
                     /locus_tag="KR250_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small basic family protein"
                     /protein_id="HBH0066048.1"
                     /translation="MLWVLGGLAFGVVVGYYIPFTYSMDYSIYMSVAILAIMDSIFGA
                     IKSNFEDNYDNVIFITGFIGNAILAILLTYIGEKLGVSLYYVAVLVFGMRLFNNLSII
                     RRYIISNIMNKKRKN"
     gene            93957..95114
                     /gene="ftsZ"
                     /locus_tag="KR250_000076"
     CDS             93957..95114
                     /gene="ftsZ"
                     /locus_tag="KR250_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HBH0066049.1"
                     /translation="MLNFDVELEECAQIKVIGVGGGGNNAVNRMVEAQLKGVEFISVN
                     TDKQALYTSKAEYKVQIGEKLTRGLGAGANPEVGKRAAEESKDEIVKLLQGADMVFVT
                     AGMGGGTGTGAAPVVAGLAKEMGILTVGVVTKPFAFEGKIRMKNAEGGIAELKSKVDT
                     LITIPNDRLLQIVQKNTSMLDAFAVADDVLKQGIQSISDLIAVEGLINLDFADVTTIM
                     KDKGLAHMGIGSASGETRAIDAARQAIQSPLLETSIQGAKGVLLNVTGGPNLGLFEVN
                     EASTLVMESCDPEANVIFGASIKEDLGDEIMITVIATGFEGLQNGALDLDTKPKSSIR
                     SSLNTTVKQAVKEIEEEVIAEEKIEPPKKASIIEEDDDESMEIPTFLRRRR"
     gene            95398..96672
                     /locus_tag="KR250_000077"
     CDS             95398..96672
                     /locus_tag="KR250_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBH0066050.1"
                     /translation="MIKLKKVLSLVMVGILSVSILAGCSKKEDSKGKEGKEKLVVWSA
                     PLTDHDADAWKPIFEKFEKENNCEIEFQIVPWDNYAEKYATAISAGEGPDIGYMYAEM
                     FPQFIEMGAVEDLTPYLEKSGTSDNYLYLDDAKMMGGIYGLPIEAANPGVLYYNKDIL
                     EKLGEKPPKTWDDFKRICEKATKDTDGDGKIDQWGLAQGWGSKVFGSLNWNWYPYLWQ
                     AGGDIYNDDLKSVKFNNAEGLKAANFINDMKKYIPEDALSKDSNEMIESVFGPGKAAF
                     TIMLSSAATSTFDKSFPDLNWGYVTSLEDKTVGSFASVDDLTLMSACKNKELGFKLLE
                     YMLSKESMEAFHKEIPRAPVAKGEKYQGDPRFEEMVTRDKHMYRPLTVGPHGVEIYEY
                     LWKQLQMMIAGDITPEQALNESAKYANGLLAQ"
     gene            96962..97834
                     /locus_tag="KR250_000078"
     CDS             96962..97834
                     /locus_tag="KR250_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E processing peptidase SpoIIGA"
                     /protein_id="HBH0066051.1"
                     /translation="MFKGFKGGYMVYIEYYVIENLVINYIIISCTSILTKRYNPKKNK
                     LLGASLGGAYSVAYLYPSMNILFTVPFKIIIMSIITLISFRSKSKKDYVHVALVFYLV
                     NIFISGSTYFIIYFTGISHLKISFLIACTYVSCELLKYIYKDLKMLKYIKELTKTINI
                     NLLEKSFCCKALVDSGNLLKDPISQSDVIIVKSSVLEGFIPEKLLNINYEDIDMKKAQ
                     EIIGSLDDKTSGRVRIIPYKHAGSNKTSIIIGLKADYIEVDEQKIGNIILGISNFNDR
                     EYGAIMNPNILPGF"
     gene            97859..98581
                     /gene="sigE"
                     /locus_tag="KR250_000079"
     CDS             97859..98581
                     /gene="sigE"
                     /locus_tag="KR250_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigE"
                     /protein_id="HBH0066052.1"
                     /translation="MLRLKERIISFITMLGIKLKIIKPKGIYYMGGANILPPPLKPEE
                     EMELLQKLETDESVKSILIERNLRLVVYISRKFENTGIDVEDLISIGTIGLIKAVNTF
                     KLNKNIKLATYASRCIENEILMYLRKNNKKKTEVSFDEPLNIDLDGNELLLSDVLGTE
                     NDEIYKIIEEEIDRDLLVMALDRLSDREKQIMELRFGLIDKGIEKTQKEVAGMLGISQ
                     SYISRLEKKIISRLQKEMKKFV"
     gene            98673..99446
                     /gene="sigG"
                     /locus_tag="KR250_000080"
     CDS             98673..99446
                     /gene="sigG"
                     /locus_tag="KR250_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigG"
                     /protein_id="HBH0066053.1"
                     /translation="MQVNKVEICGVNTSELPVLKNKQMKELLLQIKNGDEEARQQFVR
                     GNLRLVLSVIKKFNNRGENIDDLFQIGCIGLIKAIDNFDLSQNVRFSTYAVPMIIGEI
                     RRYLRDNNPIRVSRSLKDIAYKALQVRERLIRTNSKEPTVSEIAKELELEVESVVMAL
                     DAIQDPISLFDPVYQDNGDAIFVMDQVQDKKDTDENWLQEISLKEAIKKLNSREKLVL
                     DLRFYKGRTQIEVADEIGISQAQVSRIEKNALKNMRKYV"
     gene            99550..99813
                     /locus_tag="KR250_000081"
     CDS             99550..99813
                     /locus_tag="KR250_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HBH0066054.1"
                     /translation="MIRVSDIMEKEIINVKNGKRMGFIIDIDMDIHEGKVVSFTIFGD
                     GSRSFFSRGGDEEVIFWNDILKIGCDTIIVNIGSELSLDEFEI"
     gene            99835..100296
                     /gene="nrdR"
                     /locus_tag="KR250_000082"
     CDS             99835..100296
                     /gene="nrdR"
                     /locus_tag="KR250_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HBH0066055.1"
                     /translation="MQCPYCNYKESKVIDSRHTDLKSIRRRRECESCKKRFTTYEKIE
                     TTPLMVIKKDNSREYFDREKIKYGLLKACEKRPVSIDEIESIVVHIENEINKCFIEEI
                     ETKKIGEMVMDKLKELDEVAYVRFASVYRQFKDINTFVNELKSILIEKGDK"
     gene            100293..101054
                     /gene="pgeF"
                     /locus_tag="KR250_000083"
     CDS             100293..101054
                     /gene="pgeF"
                     /locus_tag="KR250_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HBH0066056.1"
                     /translation="MMKDYINIKNVQDIKEYVNLSGNCEELDEKLNSVNLVITCKNID
                     AKNREDMLKVCKECDLTFENLTSNSQIHSDIVNIVNKDTIGKRRDGDALITNLEKVPL
                     LLFTADCVPISIIDAKNKAIGLAHAGWRGTFSNIGHKTIKLMSECYKTVPEDLVCIIG
                     PSIGPCCYEVSEDLVEKFNTILTNNDEKFYIIKEGSYYLDLWKINEYMLRCSGVKKEN
                     IINLNLCTSCRADKFHSYRKHNKASERIGTVLQIK"
     gene            101069..101761
                     /locus_tag="KR250_000084"
     CDS             101069..101761
                     /locus_tag="KR250_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0066057.1"
                     /translation="MNTKVLVIDDEMHIVELLKFNLEVSNYEVSYSYDGFDGFIKAKE
                     IKPDLILLDWMLPNISGIEVLRKIRSDKDLKNIPVIMLTAKNMENDKVEGLEIGADDY
                     ITKPFSIKELLARISSVLRRYNLTSLGEENNILTTGNLKLDLSKHEVTKGSEKIELTL
                     KEFELLKLLIQNKGKVLSRNYLLDKIWGYEYYGETRTVDVHIRYLRKKIEDEDKSEKY
                     IETIRGVGYKID"
     gene            101790..103079
                     /locus_tag="KR250_000085"
     CDS             101790..103079
                     /locus_tag="KR250_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBH0066058.1"
                     /translation="MEDLNSIYFFIVMLSIAIVILAVRYTITLRKYLKEFIYVSKKVS
                     NNEYHARLNISAKGELGELARNFNHMIKNMDKTLEEVQYKHLQLRSILKSISHGILAI
                     DVNGNILLINDEAKEMIKSEPNQLVEGHNINVVIKEEEILKQILQFIGSKDNETANIT
                     TKDDIVYKIKVDPVYLQNTNNVIIGSIVNIEDITKKVKLENMRSDFVANVSHELKTPL
                     TSISGFVETLKSNENIDVNTRNRFLEIIESESDRLKRLIDDILLLSFVENKDTLLMEN
                     VVVYDVFKEVYEITQHSASEKNIEVLYKCDDKSISILSNKDYIKQVFLNLVDNAIKYT
                     QDNGIVQVVVLKDEEKIVIKVIDNGVGIPKEDVERIFERFYRVDKARSRDVGGTGLGL
                     AITKHIVKSLNGCIKVKSELGIGSEFIVTILQKNFLK"
     gene            103134..104066
                     /locus_tag="KR250_000086"
     CDS             103134..104066
                     /locus_tag="KR250_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIEGIA family protein"
                     /protein_id="HBH0066059.1"
                     /translation="MEESLLNANLFRHSFIVALVVGILCRGLVLRVTDKQYPSRPQDY
                     LEQIIISGLSASLGAIALPALIDKEFAALTFFAVAIQQFQGLAEQERITLKNIDNEEL
                     VPKGDAYVEEIASTYESRSYISLFSALVSSIVYIVFARKYGLSFFYCTILAIVSGAIV
                     GLIFRRFLRRNSIADIADIVPAKISFEGPILMVNGVIITNIGLESTREKYRNECLAIE
                     VIPRDLGAFGIVNDIGQRQAIIHNLFIHMGIDRDVDEVDIVAISKTNLEKSTVVIPYM
                     PILKDIDVMIDVVKSTPIIETSKGKQSDFSRKTL"
     gene            104111..105043
                     /locus_tag="KR250_000087"
     CDS             104111..105043
                     /locus_tag="KR250_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIEGIA family protein"
                     /protein_id="HBH0066060.1"
                     /translation="MDRVFFVAVIIGITSRIIMLNLDQKQYPTQPNVLLSQLILAFVA
                     SSLGALLVPALINRSYTSITFLSLAAEQFRQVRANRRNTLQNLEEVQLVKRGAAFIEE
                     IARTYEVRNYMCIVTSFLTIGLYYVILAETKISEGMSLVISSICGLVLAFILKKLLTR
                     KSIGDIADVMPAEISFVDESILKVGDLKGITNIGLKSDREVYINRGVGIQIIPKNKDY
                     ANAGILFDSGQRQAIVYNIYSRLGLYGDYGEPSFVPLPRRNKEDESIMIAYLPVDKNV
                     EKVMEAVKSCPILSSSKGKNLSLKNYKIGKKGSA"
     gene            105045..105245
                     /locus_tag="KR250_000088"
     CDS             105045..105245
                     /locus_tag="KR250_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0066061.1"
                     /translation="MGMDIGLNEYTLAIVTLDKNMKNGGGCPIFYANDEKDLQNKALL
                     MSKCVGGMVHDMTNGTLIIVKH"
     gene            105483..106811
                     /locus_tag="KR250_000089"
     CDS             105483..106811
                     /locus_tag="KR250_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HBH0066062.1"
                     /translation="MEVNVKKVNNIISKVYKDSIAEEIGIEVGDLLISVNEQPIHDII
                     EYRFLLSDEYLDVEIQKKDGEVYIYEIEKDYDEDLGVEFTNPIIDQAKSCRNKCMFCF
                     IDQLPEGMRETLYFKDDDSRLSFLQGNFVTLTNMSEEDINNIIKYRISPINISVHTTN
                     PELRQKMISNKFAGKLYGIMKRLADTHIEMNCQIVLCPGINDGKELDRTIKELAQLYP
                     YVNSVAIVPVGITKHRENLVELNIFNDKSASKTIEQIHQIQQKYLEKLGTRFAFLSDE
                     FYILSNSELPGYEEYEGFLQFEDGVGMIRKLKTEIEEYLNILPEKILKREKKVSIATG
                     HSAYEFIQSMADAMMDKFKNLQVNVYEIKNKFFGETITVSGLLTAKDLKEQLEDKELG
                     EALYITRSMLKADEEIFLDNIELNQLEELMRIKIIPCLNEGKDFVDKILK"
     gene            106845..108164
                     /gene="der"
                     /locus_tag="KR250_000090"
     CDS             106845..108164
                     /gene="der"
                     /locus_tag="KR250_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HBH0066063.1"
                     /translation="MSRPVVAVVGRPNVGKSTIFNKFAGKRISIVENTPGVTRDRIFA
                     EVEWLDKYFTLVDTGGIEPDSEDIILSQMRNQAMLAMDMSHVILFIVDGKAGITAADK
                     EIAQLLRKTKKPVILVVNKIDSQSQFDNIYDFYELGFGTPFAVSGANSMGFGDLLDEI
                     VENFPAGLDTEYEEDIIRVAITGKPNAGKSSILNKILGEERVIVSPIAGTTRDAIDTY
                     FEKNGQKFLLIDTAGLRRKSKIYETIEKYSVIRAMSAVDRADVVLIVIDALEGVTEQD
                     TKVAGIAHDEGKGCIFVINKWDLIEKDNKTMSNYTKDIKEKFPFMMYAPIVFVSAKTN
                     QRMNKILDTVEYVSNEHSKRISTSALNEVIGEAVMLNQPPSDKGRRLKIYYGTQTDIR
                     PPKITLFINDKDLTHFSYQRYLENKIRENFGFEGTSIKFEYRQKNKK"
     gene            108199..108813
                     /gene="plsY"
                     /locus_tag="KR250_000091"
     CDS             108199..108813
                     /gene="plsY"
                     /locus_tag="KR250_000091"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HBH0066064.1"
                     /translation="MEIFSYIIIAVVAYLLGNISTSYIVAKRIAGVDIRTQGSGNAGS
                     TNVLRTLGKRAGAMTFLGDVLKGVMAVLISEFAARLVGIDTLLAGYLAVICVVAGHNW
                     PAVLGFRGGKGVATSLGAMLAVNPVITLMCLAVFILVVAITKYVSLGSVVGIGCSPIF
                     MIMVKNKAGLIVALFLTASVIYNHRANIKRLLNGTERKIGQKKE"
     gene            108844..109863
                     /locus_tag="KR250_000092"
     CDS             108844..109863
                     /locus_tag="KR250_000092"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HBH0066065.1"
                     /translation="MEKVCVLGTGSWGSALALGLAKKGNDVSMWTRKEEQAKKINRTK
                     ENTDYLPGVLFPNNITISTDIEKTIKDCKVIVLAVPSQAVRSTCQKIKPFIKEGQVIV
                     NVAKGLEKGTGLRLSQVCEEELPQNPYVILSGPSHAEEVARDIPTTVVVASKDLKIAQ
                     MIQDLFMSPKLRVYTNPDIVGVELGGALKNIIAFGAGICDGLGYGDNAKAALMTRGIS
                     EMSRLGIAMGANMSTFAGLSGIGDLIVTCTSMHSRNRRAGILIGKGMSLEDTLKEVKM
                     VVEGITATEVAHDVAEKLDIDMPITNAIYSVIKKGSNPKEVGIELMMRSKKHEMEEVV
                     LGDDI"
     gene            complement(110020..110670)
                     /locus_tag="KR250_000093"
     CDS             complement(110020..110670)
                     /locus_tag="KR250_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBH0066066.1"
                     /translation="MTNKELQKKRILMYFIEAAQNIMENEGIENITLRKVADMAGYNS
                     STLYKYFKNLDHLISFASIKYFKDYNLNISRCIENVNDEYKKYIIMWKLFCKHSFDNA
                     QAFYQVFFNLSSDELSYITKQYYDMFPEDLGIHDSDISLMITGESIKERNKILLNNLV
                     DTGYIPAKDLDIINDIIISFYQNLLFLKKNTGKNSDDDELTMKMISSIEYIIKHAK"
     gene            110989..112128
                     /locus_tag="KR250_000094"
     CDS             110989..112128
                     /locus_tag="KR250_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBH0066067.1"
                     /translation="MSNNMRLERLRKYMNEKSIEIALIFEPDNQFYISGFKAITYSRP
                     IVTVVTQDKIELIVPGLEELHAKEVAKVDNVYVYYEIPEMKEHGISHKHYLDIILNKY
                     PKGTTVGIEKDIVSASFSEYITDKSFAIKDVGSKIFEMRYVKDAKEIEFLKIAGYLSD
                     IGIKGSLENVRVGMSELEFDVEGDNALLKYVSENYPDTYIGFANWTCSGIDRTAQPHL
                     DSNTRILQRGDIVIHSRQVWYENYRAENERTFIIGKPTERQKEVFKIAVEAQQAGLDT
                     IKAGIPARMVDEAARAVVAKYGLELYSNHRIGHGLGLSEHEEPYLRFDNELILEEGMV
                     FSMEPGIYIPGVGGFRHSDTAIVGKNGATIITNYPRSVEELILDI"
     gene            112180..113589
                     /locus_tag="KR250_000095"
     CDS             112180..113589
                     /locus_tag="KR250_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBH0066068.1"
                     /translation="MKKKIGFWDLVFMNISALFGIRWIAKSTASSFGLGLGSIPVWLL
                     FAFIFFVPASLICAELAATYPKDGGLGEWVKQAYGEKWGFMVSWLNWTSKIFWYSSFL
                     TFLAINIAYMLGNPDLSNNKMFILALSLIIFWLLSLVSTRGMAFGKFFTNTGALGSTI
                     PAILLIVMAFMSVVILKKAPSASIYTIQNIIPKIDANSLVSISAIIFALSGAETTANF
                     ITEMDNAKKNFPKAILTVAVLIGGIYILGSVAITMILPPDKIAASTGILDALAKVAQD
                     LGIGSWFIRIVAFGITLSVLGALILYIAGPVKMLFGNVREGVFPKQLTVTNKYNIPSN
                     AVIVQAIIVSLLLVGTNLLPSVDNIYNVLVTMTALTALFPYVLLFTSYIRLRKTRPNE
                     ERPYSIAKSDSVCINIARMVLVVTVVGILFSTAPVMETLKDNIIYEIEMIGGGLFVII
                     TGLMLWKNYEKKISNNSGR"
     gene            113773..115068
                     /locus_tag="KR250_000096"
     CDS             113773..115068
                     /locus_tag="KR250_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBH0066069.1"
                     /translation="MSVEVNKPSIWKNFKVLIILFVAGAFIYALPYLKNYYYDTFAQA
                     FNLTNTQMGSLGSIYGLLAMVSYLFGGFLADRISARKLLSGSLIITGISGLVLTLYPP
                     YIVVFLIHALWGITTILTFWPALVKAVRMLASENEQGKAFGFMESGRGITNAIHLSLV
                     LILFGYISSKVSDKSGLTAIIVLYSAINVIVGVLVFFKLKDNEKEVSAKFDKEAFFAV
                     IKMPHTWIITIIMFCSYSANMSFYYFTPYSTEAFGATVVFASAISILAQYCRPVASAA
                     SGLLGDKVGSSKIISYGFILMIAGLLGVIFTPTKSSMIYILLIGCVAIYVSMYAIQGL
                     HYSLLEEGNYSLSISGTAIGIVATLGYLPEVLCPLAAGKILDAFPGVTGYRYYFIVLT
                     IVAIIGLVFTFIWMNMTKERRKELIEMNNNSNENTTKVS"
     gene            115240..116715
                     /gene="spoIVA"
                     /locus_tag="KR250_000097"
     CDS             115240..116715
                     /gene="spoIVA"
                     /locus_tag="KR250_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage IV sporulation protein A"
                     /protein_id="HBH0066070.1"
                     /translation="MNNNIYEDISKRTQGDIYIGVVGPVRTGKSTFIRKFMEKLVIPN
                     IDNEFKKDRTRDEIPQSGSGKTIMTVEPKFVPADGVEIKIKDTVSLKVRMVDCVGYIV
                     EGALGHEEGGKQRLVSTPWSQEAMTFEKAAEIGTKKVIKDHSTIGIVVLTDGSVTGID
                     RKSYVEPEERVIQELKNLKKPFAVVLNTLSPKSEETSMLRSELEEKYEVPVLPMNVVE
                     MEEEDIEEVMEAVLYDFPLTEIRINLPKWVEGLERNHWIKSSIITTLKQSIIDIGKIR
                     DIEGIIQGFSELEFLEDTGVDNVELGEGVINIDLQTKQDLFYNVLEEKSGFKIEGDYQ
                     LLSLITRLSKVKNEYDKIESALIDAKIKGYGVVAPSLEELSLEEPEIMKQGKQYGIKL
                     KANAPSLHIIKADISTEVSPIVGNQNQGEEMIKYLMEVFEEQPADLWESNMFGKSLHD
                     LVKEQLQSKLYTMPEEIRVKMQKTLQKIVNEGSSNIITILL"
     gene            116898..118853
                     /locus_tag="KR250_000098"
     CDS             116898..118853
                     /locus_tag="KR250_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0066071.1"
                     /translation="MKKYEIFIFMSIIVLCFISFPHKVFGQEENCLEDFERWVQENKN
                     NKEEIVYTLSCDMVIDEEFRFYIPYDSNFTIDTNKYKILIKNHGRFIIDDNELNIIGE
                     GGKEGVIHIENGGSISIGINNIIATDGTALYVEEGGDLHIKSSYESVEKIKANGKDAI
                     GIYTENDIRILNKDIEVNGEHAIGVYSKGDVEIEETSIKAYSSNNESGLLRDNNKLAQ
                     SIISESKKVYIIDDYNELIPSIAEDSGYDIVKCCHRGIGVFSDEIKVSKDDKIENIKF
                     PKYMVLETSSGNRLDIDVEWDFTDYYEKLEKGENFNITGKFKTEMLNKEKIIINDDVV
                     PILNVSVIEKKPIDNLELEFQNTKNGYVAVLFYDMPYSASKVFVEYSSDGINWISEEQ
                     EDIRDQAILFFEDFKLRCFRVKVVGGLKEGYSNIVFKPGFIMGGGDNQETPDDEREND
                     DIDGDRGGGGRDDPDRDEDDNNQDGNNGNENNDSNNNQNNNNENNNNENDNSDEKQPN
                     TPKDDVESSTDSNENKNPHDIPKDGNKENNSDKEKGFYNKNSITENDDNYDLSSKSVF
                     GTNEHSFPYRESISNKNQDTNKNKSNISDYDGIINNNYKLESKEYNLKKDIIFLFCIL
                     TPIMIGSILIVNPDTRKSIIKFIKLKK"
     gene            119143..119412
                     /locus_tag="KR250_000099"
     CDS             119143..119412
                     /locus_tag="KR250_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HBH0066072.1"
                     /translation="MKAILTVIGKDKPGIVAGISDELYKQNINIVDISQKILQDYFTM
                     IMIVDLEKSLNSFEKTVEDLVERGKKLSVDVKIQREEIFNSMHNL"
     gene            119438..120793
                     /locus_tag="KR250_000100"
     CDS             119438..120793
                     /locus_tag="KR250_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009170073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HBH0066073.1"
                     /translation="MNVGNILETIKMIDEEQLDIRTITMGISLLDCIDPDGDKAREKI
                     YNKIMSSAKDLVKVGKDIEREFGIPIVNKRVSVTPISIIAGATDENDYVKFAQTLDKA
                     AEDLGIDFIGGFSALVQKGYTKGDKILIKSIPKALASTNKVCASVNVGSTRCGINMDA
                     VKEMGEIIKETAELTKDAKGFGCAKLVVFCNAVEDNPFMAGAFHGVGEADRIINVGVS
                     GPGVVKRALEKVKGEPFDVVSETVKKTAFKITRVGQLVAKEASSRLDVPFGIIDLSLA
                     PTPAIGDSVANILEEMGLEVVGTHGTTAALALLNDAVKKGGVMACSHVGGLSGAFIPV
                     SEDAGMIDAVIKGALSIDKLEAMTAICSVGLDMIAVPGNTTAGTLGAMIADEAAIGMI
                     NNKTTAVRIIPAPGCDVGDMVEFGGLLGRAPVMPINTNSSELFTQRGGRIPAPIHSFK
                     N"
     gene            complement(121256..122296)
                     /locus_tag="KR250_000101"
     CDS             complement(121256..122296)
                     /locus_tag="KR250_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBH0066074.1"
                     /translation="MSEILLGTGLLLLVLCCFTIFSYKAPYGMKAMGALANAACATFL
                     VEAFNLSLLGDVLGMEFFRSVGAANGSLGGVAAAILVPLALGVSPVYAVLIGLSCLDF
                     SILSGFVAGYFVSFLIKFIEKKVPPGVDLVVVILLCAPLSRLIASNVDPLVNSTLLQI
                     GQVLIEATNISPIIMGIILGGLITVVGTSPLSSMALTSIIGLTGLPMAIGALGAFGSA
                     FMNSTLFAKLKLGSKKDIISVAIEPLTQSDIISANPIPIYSTNFFGGAFSGIIVALMG
                     LVNMTPGTATPVAGFAIMFAYNPVQQVLITAGLCALFNVIAGFLGSKIFSTFKVLTAG
                     EIRGTDVEEEIA"
     gene            122677..123312
                     /locus_tag="KR250_000102"
     CDS             122677..123312
                     /locus_tag="KR250_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0066075.1"
                     /translation="MKKVKYTRLVILGILIYLVFNILVMIISKNVDTLVLKNEKVDAK
                     IHVKGTIEKDANDQSNYLSTYISKEDSNNFEKNQNVIIEYHEQKLDAKVYKIYENNNK
                     IMVKLKISNQIVANQDTSVEEFDIIYNQMECLKIPKTSIKTKDNKRGVYVIDEQSQSV
                     KFVILEGITYENESSVFVDYYKNDINGVTSVNLYDKIILRPNSINTNIRIK"
     gene            123322..124026
                     /locus_tag="KR250_000103"
     CDS             123322..124026
                     /locus_tag="KR250_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HBH0066076.1"
                     /translation="MGSIKDNLSEIRNKIDKAAKKVDRDSKEITLLAVTKTVDVDLVN
                     EAMREGITSVGENKPQELARKYDIIGDKLNWHLIGTLQTNKVKYIIDKVCMIHSLDRI
                     ALCEEIQKRAEQINREIDCLVQVNISKEESKHGLDREEVVDFVKEVSEKYKNIKIKGL
                     MTMAPFIEDEDEIRGVFKGLKDLSKQIKELNIPNVGMDVLSMGMSHDYEIAIEEGATI
                     VRVGTSIFGERNYNKK"
     gene            124066..124524
                     /locus_tag="KR250_000104"
     CDS             124066..124524
                     /locus_tag="KR250_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HBH0066077.1"
                     /translation="MSNGIISKFKNWIVDEEEDYIEDEYESGMDDIVQEEEMNSGFST
                     AKANKIVNLHTTSQMKVVIVEPKVYDEAATIADHLKQRRAVIVNLEGLTNSEVRKSIF
                     NFMNGAVYVLDGSIQKVSKSIFILAPNNVDIDANMKKELESKAFFPWQNK"
     gene            124543..124800
                     /locus_tag="KR250_000105"
     CDS             124543..124800
                     /locus_tag="KR250_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HBH0066078.1"
                     /translation="MGTIRIALYYLLDIIAWMIVIKSLMTWFPNGRQSKIFDILENLT
                     EPIEGPIRSIMYKYTNGPIDFSPLIAIVLLMFLRQLVLVIF"
     gene            124810..125595
                     /locus_tag="KR250_000106"
     CDS             124810..125595
                     /locus_tag="KR250_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HBH0066079.1"
                     /translation="MDKLKLTNHIKDIDLKNKMFKVIDKANSCIKNYDVKSTEFLNPY
                     EVKNAVAILNSTNEIKYSVDGGYEQAERSTVFIYPFYMEYEDIEDTLRFLQIEGNFKF
                     KNISHKDYLGSILGLGIKREKIGDIIIHDSFCQVVVSSDICDFIIVNLEKVSRNNVIV
                     KEISRENIVNSSSKYKEVSFTVSSDRLDCVISGIYNISRQDSAKYINGEKVHVNYEKI
                     TSTSKIVKNDDLISIRGKGRAKVTQIGDITKKGKIKVQARLIV"
     gene            125630..126157
                     /locus_tag="KR250_000107"
     CDS             125630..126157
                     /locus_tag="KR250_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HBH0066080.1"
                     /translation="MLTPIEIENKEFKKGLRGYRDEEVDEFLDIIKEDYESLCRENTA
                     LKEKLGLYQDQVNKYENIEETLKATLITAQSAAEDTCSAANKKAKIIVEEADLKARQI
                     IEQANNRVIEIRKEYDALVKEFKIFRNKFKSLLEDEIRSVDEIFYDVDEKCVNAFEGT
                     TVYNYSNDEAATTLE"
     misc_binding    126332..126564
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            126657..129764
                     /locus_tag="KR250_000108"
     CDS             126657..129764
                     /locus_tag="KR250_000108"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HBH0066081.1"
                     /translation="MAKFKPLVDSSVKQAEAQVFDYWKDINILEKTLEKGKDDPSFVF
                     YEGPPTANGNPGVHHVLSRTLKDSVCRYKTMSGYQVKRKAGWDTHGLPVEIQVEKELG
                     LTSKQQIEEYGIAEFNQKCRESVFSFEKQWRIMTERMAYEVDLDNPYITLDNNYIESV
                     WWILNKFNKEGYIYEGHKILPYCPRCGTGLASHEVAQGYKEVKTNTVIAKFKKKDADE
                     YFLAWTTTPWTLPSNVSLTVNADVDYLKVKKGDEVYYVSKPLADKVLGEDYEVLEEMK
                     GKDLEGLEYEQLMPFVEVDKKAFFITVGDYVTTEDGTGIVHTAPAFGEDDYNTGKRYD
                     LPVIQPVDETGKFTSTPWEGRFVMEDGLDVEIIKWLASENKLYSKEKVVHNYPHCWRC
                     QTPLVYYAKPSWYIEMTKLKDKLIENNNGVKWFPSFVGEKRFGNWLENLNDWAISRTR
                     YWGTPLPIWRCECGHTDSVGSRAELAEKAIEDVNPETVELHRPYVDDIHLKCEKCGKP
                     MTRVTEVIDCWFDSGAMPFAQHHYPFENKENFDQLFPADYISEGIDQTRGWFYSLLAV
                     STFVLGKAPYKSVLVPDLVLDKDGKKMSKSRGNTVNPMELFDQYGADALRWYLLYVSP
                     PWTPTKFDMDGLKEIQSKFIGTMKNVYNFFTLYANTDDINPTEFFVEYKDRPELDRWI
                     LSKFNNLMKDVEENLAIFELNKTLRMIQDFINDDLSNWYIRRSRRRFWATELTEDKKA
                     VYNTTYELLHGLCRAIAPFAPYMSEEMYRNLTGEVSVHLAEYPKCNEELVDTKLEEKM
                     DLAKNLVTLGRASREVERIKVRQPLQKVLVDGKFEDTISDVVDLIKEELNVKEVIFAK
                     DLDEYMNFSLKPNFKEAGPLLGSKMNLFVGALSKLNAHETANKLEKGETLTVDLDGEA
                     FEFNKDLVLIGITAKEGFNVSVENNLFVILDTKLTEELINEGYAREFISKVQQLRKSN
                     NFEVLDNIVIEYCGDDEIAKAVDHFNEYIKSETLALEINRVDDKSLEEQNLNDHMTGI
                     KVIKK"
     gene            130051..130923
                     /locus_tag="KR250_000109"
     CDS             130051..130923
                     /locus_tag="KR250_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBH0066082.1"
                     /translation="MYGESKKLSIDNNIFLQYCLIILGMILSTLGINLYLAPAKLLSG
                     GVAGICVILYKLFGINQGISSFLMNIPIFIIARKYFDNKFLLISFVNMLLFSLALGLT
                     QDIAKYFPIDDTMLQCIYGGALTGIGMGLTFKARATAGGLDIIAAIMKRKYDIPMKNT
                     FLFINFFVVCAGAFLFGAKLVMYTLITMYIISFTMDIGKDCFDRKKSILLISNKYDEI
                     SKVIMNKMGRGVTFLEAEGAYTQNKKKMIYCIISANEIGKFKDLIYDMDSEAFISVNN
                     VEEVKGGGFKDKFL"
     gene            131050..131322
                     /locus_tag="KR250_000110"
     CDS             131050..131322
                     /locus_tag="KR250_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0066083.1"
                     /translation="MLRERFVVRNHHNPHTSGCCGTGGEGCCGGDNHEHSHQHSHSHG
                     HEHEHVNGVGHDHNHDDGFSGTHEHSHEHTHTHGHEHSDGHTHEHK"
     gene            complement(131485..132057)
                     /locus_tag="KR250_000111"
     CDS             complement(131485..132057)
                     /locus_tag="KR250_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBH0066084.1"
                     /translation="MKTNNMKQAILSSATNLIAKNGVQNTSLADIAKDVNISKGTLYY
                     HYASKDDIIYDIADTHLEVITSAILNCVKNVKSKNSQIEMVNLILEKISTIESRGRVH
                     MYLICEAITSNNDLKERIRLKYIEWRTTLKSEIVESLEKYNSSENEQDAESFSFLLMS
                     IVDGLVVQSLLKTEKIPYENIASFLVNHWI"
     gene            132241..133083
                     /locus_tag="KR250_000112"
     CDS             132241..133083
                     /locus_tag="KR250_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBH0066085.1"
                     /translation="MDKIKLIVDSACDLPDDIIEKYNIEVVGLNVSFGEESYISGKEI
                     DNETFYRKMSESKVLPKTSCPSPDKFLEAYHCQEESVLVINISSGLSGTYNSALLAKD
                     MFEKEGNNKKVEVIDSLSGSIGIGQLVLKAAILIKEGKEIEEIVSIINKYKDNPPFFG
                     TLETLENAIKGGRINPIAGKIINTLNFKAIIQVADGVVTPIDKARGEGNSLKKLITLV
                     ESKIKDKEEKVLFIGHANCPEKAQKVREVMEKDVKYKDVVICEVGSVMGTYTSKGAIL
                     ITAI"
     gene            complement(133200..134276)
                     /locus_tag="KR250_000113"
     CDS             complement(133200..134276)
                     /locus_tag="KR250_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBH0066086.1"
                     /translation="MKAQRLNAVLEQMKKDDISQMLVSDPTSIFYLTGVLIHPGERLL
                     ALYLNLNGNNKLFINELFPVSEDLGVEMVWFNDTQNPVEIITEHIDKNATMGVDKNWP
                     ARFLLNLIELGGGSKFVNSSYIIDTLRMCKDEEEKELMRIASKLNDKAMEQLKATVSG
                     ELTEKQLVGKLSKIYEDLGTDGFSFDPIIGFGPNGANPHGEPGNALVKPGDAIILDIG
                     CIKDNYCADMTRTVFYKEIPEKGREIFEIVLEANKRAEAIVKPGVRFCDIDAAARDYI
                     TEKGYGQYFTHRTGHSIGLEVHDKGDVSSINTDTVQPGMIFSIEPGIYLPGEFGVRIE
                     DLVLVTEDGCEILNKHDKEICVVG"
BASE COUNT        52165 a        15205 c        23265 g        43851 t
ORIGIN      
        1 taacttttaa aaattagttt tgctaattta caataaaata ggtgtgctgg tgtggctcaa
       61 cggtagagca gctgacttgt aatcagcagg ttgtaggttc gattcctatc accagctcca
      121 gtaaagacta tgtttttagc atagtctttt tttattgttt tttattgttt gatgtatttg
      181 ttattttgta ttaaatactt ttagtggtct aatttacaaa ttataggtat aaaagatgac
      241 acagtttaaa attattgttt taaactgtgt ctgtgagttt taaatcaatc taccatgaat
      301 caccattctg gcatcatcaa attcataggt acatgtagaa agtgtaacta ttttatcatt
      361 gctatttacc ttaataggag atttatacaa tgatttagaa gtaatatcat ttatgtaatt
      421 ttgataatca gattcattgt taaaatttgt ttttagataa tcataatcag attctacaat
      481 ataagcagaa aaaacatcat ataaaaattc ttttccattc aaggttattt ttattttatt
      541 atttttttta aagaagtcag catctttaaa cttatttagg ttgttgaaca tagttttatt
      601 tttcatatta tgtccatata ttatgatatt tttgtcatca atagatttat ttctataatc
      661 cataaacagt gtaccagaaa tagaatcttt tttataaaaa tctttgtcaa gatagtaaga
      721 attatcttta gattgtacaa ctggataatt tatatttgta ttctcaacag aaagccaaaa
      781 tttaaaatca ctgttaatct ttgataaatc ctctttttta tattcttttt tttgaagttc
      841 tgaggatatt ttagtatcat gattgtattt ggtaagtttt gagtatatat taaatccact
      901 gtaaagtatt actaatacta gtataatgtt aattactatt ctatataatt ttttcaatag
      961 atcacctcga gttaaatgta gtttatatat ctatattaca ctatttttga ataaatttaa
     1021 atttttagta taatattact ttgacatgta aaagaatttg tgttaacata actaagtaaa
     1081 ataaatatta agtaagccag ataatcgcat gtaatcttac atgaggaaag tcggagctcc
     1141 atagagcagg gtgctaggta acgcctagtg ggagcgatcc taaggatagt gcaacagaaa
     1201 taaaccgcca ctattgtggt aaggatggaa aggcgaggta agagctcacc agcagctagg
     1261 cgactagttg gctatgtaaa ccccacctgg agcaagacca agataggaat aaagagtgct
     1321 gctcgtactc tccggtaggt aggtcgcttg agcctattgg tgacaatagg cctagaaaga
     1381 tgattatcta atacagaact ccgcttatag acttgcttaa ttatttcgat agtcaaaaac
     1441 atcagtttaa actgatgttt ttttatttgt aaatttgtct aatctacttt taaaattttg
     1501 aggaatattg taatataagt tagtacagat attaaaaatg gtataatata agatagatat
     1561 aaaaagaaaa agtaaaatga tgtaaataat atgatatgat ataagatgta gcaaatttag
     1621 taaataatta tttagatatc ataatcaaat tagcaacttt gcacaatgta aatattacaa
     1681 taaaataatt gatttaagga gtgaaggttt tgagtgaaaa taatataata agaaacactg
     1741 ttttaaatgt cacagcaagt tttttaaaac aagagtctaa aataaatgaa aagctagatg
     1801 gtgttttgga aaagaaattt gaaaaagtag aatttaatga agcaaaatat gcagagcttt
     1861 taaaatttaa tatattgttt tataagacat tagcaagaaa tacagaacca cttattggca
     1921 aatggatagt agataagtac atacctgaaa tagatgaatt agaaaaagat ttagagttaa
     1981 ctacagcaaa atgtagaaag tatgtaaata aggctatgaa agagggatta gactctctaa
     2041 aagccaatga tttaaatagt tttttagctt atgataaaat ggaactatca gagagaagaa
     2101 gacgtttaga aaaggattat aaggttttaa acctatataa agaccttctt aatatatctc
     2161 ttagaaaaat aagtttagaa aagaaagaat gtggagattt atttcttaaa aatcaagcag
     2221 atgcgaaaag agaattaaaa agagaaatta tattctgtgt aaacaaaata ttagctagta
     2281 ataaagatgt ggtacctaat aatgtggaag agaataacga acaaaacaaa gtagaaataa
     2341 aagcaactga aattgaggat ttgcaattag gtaaagataa ttcagaaaat gacatgctac
     2401 taaatgaaat taaaaattca aaagtaaaac atgaatcaaa atcaaatgat atagaatcga
     2461 agtttataga taagttaaat agcttaaata atgaaagtat atctcaaaca atacacaatg
     2521 aatataagaa gttatgtgga ttagaagaac ttcattctgt tgaagcatat ggttttggca
     2581 aagaaattat taaagatttt gcttgtgcaa cagttgtatt agaattttta aagagaagaa
     2641 atagagacct tatagaaggt gcaatgagac tgactataat tggagaattt ggtcctgaaa
     2701 attttaagga atttgtggac tatgtaatta aaaataaaac agaaattagt gaagatacat
     2761 ggaataaagc acagaatcta ataaaagata actatagtga attagaaaat catgaaattg
     2821 catcaaaaag aactagaaga aataaagata tcgatattga agaatacatt tacatgataa
     2881 aaaatgcaga taaagatatt tgctttagaa gtagtatttc aatagaagat gatgctgaag
     2941 aagggactaa agaagaagta gatagtaata atcaagatat aggtgacgtt gtagaagata
     3001 aagatactac agataaagag tacgacagca ataaagaaga tataattgaa ccagaaaata
     3061 aaaagagtaa aaagaaggct aaattatttg gatttattaa aaaagacaat gaagaagctg
     3121 aacaagaaga agagaattta aatgatattt ctccagatat aattttagat aaaccagttg
     3181 aaaatcatca agtgaaatca gaagaaatag agcaaaatga attgaaagaa ataaaacaag
     3241 aagaaccaag tcaacatata gaagaagaac gtagtgtaaa aatagaaaaa ccaataaata
     3301 ataacttaga tgaaaaagta tcttcaaata atgaatcgaa gcttgaaaaa gaaagtaaaa
     3361 acttagaaga taaaaaagca aaagaaataa aagaagaaaa attagaaaat gaaaaatcag
     3421 tagtaatacc tattaaaaag aaagaaaata gtaataaaaa atctaaaaat tcttctaagg
     3481 acaaatatag agaaaacaag aaagaaatga aaaattatgt atcagataaa gaagattctt
     3541 tagatgatga agaggttgtt tctaagaaga gtagattaaa agaaactatt atagcagtag
     3601 ttatagtggt tattgttggt gtaggatatt ttataacagt aggcaacaat aagaaaaatg
     3661 ataaagagaa tattccaaag tcatcaactc aacaacaagc taacaataaa ttaactgaag
     3721 aagagaaaaa agcacaagct gaaaaagaga aaaaagaagc agaagaaaag gcaaaagctg
     3781 aaaaagaggc tcaagaaaaa gcacaagctg aaaagaaagc aaaagaaatg gaagcatata
     3841 aagatggtaa aggtgtatat tacactgttt atgcaggttc acttaaagtt gaaaaaacag
     3901 caaaagaaac agcaaaagaa tatgaagcta aaggtatttc ttcaacaata attcaagaaa
     3961 atggatacta taaaataaaa ataggtgatt attctcaata tggagaagca caagaaaaat
     4021 gtaatgaact agctaagaag tctattgata catatatagc tatgtatgat aagtactatg
     4081 attataagtt agaagaatta aaagaatcag ctccttcttt gagtgctgaa gaacttaaac
     4141 aaaaatatga agatttaaga agtgagttaa agaacaaatc tggatatcgt gaatatgtta
     4201 aacatttaga taaactttat gaagaaatag ttgaaggtgc ttagagcatt ataaaaaagg
     4261 agaaactatg tctcagaata aaaagtatta aaaaaaatat tatttgtttt gagacatttt
     4321 attcaccttt ataaaatact tataattctg tttaggtggt gaaggtatgt tgaaattagg
     4381 agaaaaaatc atatatgagc tttcagatgg gtttgagctt agtggcataa gtagatactc
     4441 aaaagaatat aaaaagcttc atataacaaa tctagggaat ataataatca gcaaatcaga
     4501 tgaagttaat gaggatacag gtgtaaaata tatatatagt ttttcagaca ataaagaaaa
     4561 attagaggca gttttagaag attctcaaat aattgtctat ggaaataact tgccttttta
     4621 cataaaaagt gaaaattcaa gtaaaaatgt tacagcaaat ttgactatga aaataacttt
     4681 ggatgaatat gatttaatct gcaaaagtca aagagaattt atattctatc taaaggataa
     4741 tatgactata ttaagtttag atgacaataa gttttatatg ggtggaatta ataaagataa
     4801 tgagaagttt atatttatca gtgggaaaaa tagatttgaa ataaattttg atgatataga
     4861 aagatacatt ttagaagata aaagagtgtc attgaaggga tactttcata tggaaagaga
     4921 ggggattata gtaaggtctg tatctatatt taataacaat atagatagtg tagtcccatc
     4981 tgatttaaat gagagagtta aagataacca aaaaatagga aatcttccta aagattgtga
     5041 aatagtattc tgcaagatat caggaaatat agatggattt gattataaaa acacaaacat
     5101 gcttttagta aaatatcaag accaattaat ttttataaat aagaaatcta agaagactat
     5161 tgtaaagtca gcgaaagata attgttctaa gttaaattta ggagaagata taatattata
     5221 tgataataaa aatgtattta acctacatat aaatgataaa aatagagaaa ttatgcaaat
     5281 agatgaccta aaagatatag aaaatgaaat agtgggatat acattaaaac atgctccatt
     5341 ttttattcaa gaagatttcg atagcttgac tattctaaag tcttttcaaa aagaaatcat
     5401 atctataaaa aatagtgata taaaagacat tgtaattaat aaagaacttg aaaacgaaaa
     5461 ttctaatttt gtagaaacgg aaataaaatt taataatcaa aaagtacttt taaacttaag
     5521 taaaagtatg gttcaaaaac ttatgcaaga tgtatttata tatgcaaagc aacctctact
     5581 taaagaaaat tctattgaag tgatatataa gaattggtct aaagctatga atgatatgat
     5641 aatatttaat ttctttggaa atatttacta tatgaaatct gaatttgata agattttaga
     5701 aaaagagtta aatgatgaaa taagaataga agttataaat tcactttata agcaaataca
     5761 agaacaaaga aacaatttag atttattgtc agcatatatg ccaagaatat tagaaaatca
     5821 agaaatagat ttgtttgaaa agtataatac taaacttgat gtacaagtgt ttaagcagat
     5881 taaaaatcta ttgtctgatt tatcatataa tataagcagt tatttaaatg aagttgaaaa
     5941 aagtttagac aatatcatct ttgtaatttc aggagaagat aagaagaaat acaattatag
     6001 aatgttaaaa gaaagtgaaa gtgcaagtct tgatgtattt ttaaaacaag ccatatcaag
     6061 attaaaccat ttagtagaaa atatgtatcc ttattatgta gatgaaacta gtagggaaat
     6121 gtttaagtta tttgagttat tgtggaaaaa ctatagaaat attgatgatg atagtataaa
     6181 agaaatatta ttcgagagaa taacaaatac atatgtattt aaacaattga cattaaataa
     6241 tagtacaaaa gaaagaagaa aagacataat tgaaaaaata tataactctg ttgattatgg
     6301 aaccaataaa ctagatgaaa atatgttttt cacaggagga ataaagtatg tcaagtaaat
     6361 acaatgtttg gacttttaac tatgagttct taggattgga ctcaggggat gaaaaaaata
     6421 atgaagttta ttacgagatt gatttagatg aaattttaaa tgaaaactat atagatgaag
     6481 atatagatag tggtttagat tctgatggaa atagtatttt aggagaatta atatcagaaa
     6541 tggatataga tgaggatgtc agtgttgata tgacatatca agaactagaa gttgacttag
     6601 aagatttaga ttcttatcta gatgaagata tagatagtga tataaaaggc atcttagatg
     6661 aaatttagta cctgtataag ttttggataa aatataaaca atacaatatt aacttataaa
     6721 actaaaggta gatgaaattt catctacctt atattatctt atagcattaa atattgggat
     6781 aatatagagt ttgaagaaaa agtataaata tggtatatta atttggtaca atagaaaatt
     6841 tacgaagaag ggtgaaaaaa tgcagatgac agttaaaaaa gcaataatac cagcagcagg
     6901 gctaggaact agatttttac cagcaacaaa atcacagccc aaagaaatgc ttcctatagt
     6961 agataaacca actttacagt atataataga agaagctata gaatcgggaa ttgaagaaat
     7021 tcttataata acaggtagaa ataaaaaatc tatagaagat cattttgata aatcagttga
     7081 attagagtta gaactagagc aaaaaggtaa gacagagatg ttagaaatgg taagagacat
     7141 atcaaatatg gtaaatattc attatataag acaaaaagag ccaaaaggtc ttggacatgc
     7201 aatttactgt gcaaaaagtt ttataggtga tgaacctttt gccgtattat taggtgatga
     7261 tatagtagat tcagatgttc cgtgcttaaa acaattaata gatacttata atgagtataa
     7321 aacaactgta ttaggtgttc aaaagatagc taaagaagat actaataaat atggtatatt
     7381 ggatgtaaaa catatcgaag atagagtata taaagttaaa gatatggttg aaaaacctgc
     7441 aattgaagaa gctccatctg atatagctat acttggaaga tatataataa ctcctgcaat
     7501 atttagcata ttagagaaac agacacctgg taaaggtgga gagatacaac tgacagatgc
     7561 acttcaaact cttggaaaac aagaggctat ttatgcttat aactttgaag gtagaagata
     7621 tgatgtggga gataaaattg gttttttaga ggcaacaata gactttgctc taaagagaga
     7681 aaatttaaaa gatgatttaa tggactatat gagaaaaaaa gtaagtgaaa aataaatatt
     7741 agaaaagagg taattacaat ggcagtttta gtagctggag gagcaggata tataggaagc
     7801 catacagcaa tagaactttt agaatcaggt tatgaagttg ttatagttga taacttaagt
     7861 aatagcaact caatagtggt tgatagaatc aaggaattat ctaaaaaacc agtaaaattt
     7921 tataatattg atatcagaaa taaagatgaa atgcatgttg tatttaaaga aaataatatt
     7981 gaatcaatta tacactttgc agctttaaaa gcagttggtg aatcagtaga aaaaccaatt
     8041 gaatattaca gcaataatct aataagtacc ttaaatttat ttgaattaat gagagaatat
     8101 ggtgttaaaa agtttgtatt tagttcttca gctactgtat atggagaccc acatacttgt
     8161 cctatattag aggattttcc actttctgta accaatccat atggaagaac taaacttatg
     8221 atagagcaga tgttagtaga tatatcaaaa gcagataagt cattggatat agctctttta
     8281 agatacttta atccagtcgg tgctcataaa agtggtagaa taggtgaaga accaaatgga
     8341 gttccgagca atctaatgcc ttatataaca aaaatagcag taggtaaatt gaaggaattg
     8401 agtgtttatg gtaatgacta tccaacacat gatggaactg gagttagaga ctatatacat
     8461 gtattagatt tagctgctgg tcatgtaaaa gcattacaaa aattagaaga aaatccagga
     8521 cttgttgttt ataatttagg aactggaaag gggtatagtg ttcttgattt agtaaaagca
     8581 ttttctaaag caagtggaaa agaaatacca tataagattg taggaagaag ggctggagat
     8641 gtagccatgt gttatgcaga ttcttcaaag gcagaaaaag agcttggatg gaaagctaag
     8701 tatgaacttg aagagatgtg tgaagattca tggagatggc aaagtatgaa tcctaatggg
     8761 tatgaagagt agaatacata cttatatgta taaatgtaac aaatttgtaa atataaggta
     8821 ttggtaattt tatgttataa ttagacacat gtagtaatgt ttctaaaaga gggggaagtt
     8881 atagtgaaaa aaagtctaaa aaagtattta gtactagcac tgactttagt tttagtattt
     8941 gcatgtggcg taccagaatc aagcgcagcc agtaaacatg tgattatagt aaattcaaga
     9001 aaaaatactc taggatattt tgtaaataat aaacttgtaa aagaatttag agttgcaaca
     9061 ggtaaaaagg ggtcagaaac accaacaggg aaaacaaaag tagttaacaa gataaaaaat
     9121 agaccatatt ataaaggtaa tatacctgga ggaagtccaa gaaatccatt aggagataga
     9181 tggatgggat tagcattaaa aggaacttat ggagatacat atggaataca tggaaataat
     9241 aatgagagtt ctataggaaa gcatatttct ggtggatgta taagaatgca caataaagat
     9301 gttagatggt tatttgacca agttcctgta ggttcagatg taattataga ttatagtaac
     9361 gacagctatg taaagatagc ggcaaaatat aagataaatc taaatcaaac aggatggaaa
     9421 acagaaaatg gtaagaagta ttatgtaaaa tctgatggaa cataccaaaa gaatagttgg
     9481 ctaaaagtaa atggaaaaat gtattacttt gatgcttcag gagtaatgca aacaggatgg
     9541 aaaacaatta ataacaaaaa atattattta ggaactgatg gagcaagagt atctggatgg
     9601 aaagtaatag acggaaaaac ttattacttt aactcagatg gagtaatgca aacaggatgg
     9661 caagaaaaga atggaaagaa atactatcta ggatctgatg gtttagcagt aacaggatgg
     9721 caagaaatag atggtaataa gtattacttt gacaaaacag gaataatgca gacaggatgg
     9781 caacaaatag atggtaagtc ttactattta gacaaagatg gaaagatgct aactggttct
     9841 caaaaaatag atgggaaaga ttatactttt aatgaagatg gaactataaa tcctacatgg
     9901 gatacaatta taggtgcaaa tagatttgat acagctaaaa agataagttc agttggaaac
     9961 tggaatgctg atagttcaga tactgtaata ttagtaaatg gtaatgcaat agctgatggt
    10021 ataacagcaa ctcctttagc aagtagctat gactcaacaa tactacttac taatacagct
    10081 aatttaccaa ctgaaactgt tgaagaaatg aagttattag cacctaaaac agtaatcttg
    10141 ataggtggag aaaatgcaat aagttctaaa ttagagcaag aaataaaaac tactttcaac
    10201 acagaaacaa agagaatagc aggtcaagat agatatcaaa ctgctacaag aatagctgaa
    10261 gaacttggaa atcgagaaga aataaaaact gcatatatgg tttctggaaa tggggaggca
    10321 gatgctttat ctgtagcttc taaagctggg gaagaaaaac aacctataat attagtcaat
    10381 aaagatggaa ttacagaaga atcttataaa tggctaacag aaagaaaatt agaaaatgca
    10441 tactttatag gtggaccaag tgcaattaac gattcagtaa tagcaaaaat gaatgatata
    10501 actactgaag atatttcagg aaatagaata tatggagata gtagagtaga tactaatgcc
    10561 aaagtgatag aaaaattcta tggagatact gatttacagg ctgtattagt tagtaaatca
    10621 gatgctttag tagatgcttt atcagctgga ccattagctg taaaattaca ttcaccaata
    10681 gttttaatgg ataattcagg attatcatca gagcaacaaa gagtatttgc aaataaaaaa
    10741 gttgaaactc cttatcaaat aggaggcgga gtatcttata tagtaatgga taaattaatg
    10801 gatattcttg caaaatagta aaaatatata ttgaaaaata attataattg agatgtgaaa
    10861 ttcaatcaat aaaagcttga ttgttttaca tctcaatttt ttagtataaa attaagttta
    10921 agttatataa aaatcttgga atatttttaa aactcatata aaatgaatat agatgctatc
    10981 atcatatgtt aagagggtga gtaagattat aaagaaaaaa tatataatgt ttattgatga
    11041 aagtggaaga gctgatataa atcatgaaga cccatttaca ttgacaggag taatttttga
    11101 atataaatat gcagtaagtc aaggcgatta tgtatgttct ttgaggaaag aaatggatgc
    11161 atttaaagag tattgttttg gaaccagtaa cataggaata catttgacag atatatcaag
    11221 aaaatcaaga gcatttgaga agtttgatga ttcacagatt aagttattct atgatgagtt
    11281 gcctttattt ctatctagat tagagtgtac tataatatca gtaacagtag ataaagctag
    11341 attaaagaaa tattatgcac cttcaaagga accgtatgtt gtggctttta tacatgttct
    11401 tcagaatttt tattcatttg taaataatga aactgtagaa tctgcacgaa tagttataga
    11461 gtcaagggat gatgtctcaa acttaaaagt acaaaaggcg ttttttgatg tatttaataa
    11521 tggaacaata tacttagata tagatgaaga attaagagat aaggttaagg gttttataat
    11581 tgccaaaaaa gaagatgctg actatagaga aggtttagaa atagcagata ttttatgtaa
    11641 tcctcttagc agagcaagaa gagggttaat agaagctaat ccaaagtgta tgcaatatgg
    11701 agaaaaaaac aaaatattca aatctgtcag aaacaaaata tatactccaa cgagtgtgaa
    11761 tgatataaga aattggggat ttaaagaagt tcctattgta gagcctgttg gaccatggct
    11821 tgaaaaaatg cgttagatac aattgacaaa ttttaaaaaa tctgatataa tgtaagtaac
    11881 ctagttattc aggaactgaa tccagttgct gttgtataac gataattact gagactcgga
    11941 tgccgactgt cagttaggaa tctatacttt taaaagtata gattttttta ttttataaaa
    12001 agaataaaag aggatattta aaaataaccc ctttattaat aaattataat tgagcgtttt
    12061 taatgacaaa gtttttaaag tttttaattg caggaggcaa atatctatct tttataaatg
    12121 ctaagtaaaa gtttctttcc caacttgggt gaataatctg aattactttt acttttgtaa
    12181 agctaagcat attcatattt gggactacag caattccgaa attttttgca actaatccag
    12241 caattacttg gtcttcctct acttcataga ctatattagg ctgttgattt atttttttaa
    12301 acatatcatc aattatatat ctaagaccac ttctctgatt aaatactatt tgaggataag
    12361 gtatggtttc ttttaaatct atagtatcct ttgcagctag tggatgacta tatggtacaa
    12421 ttagtactaa atcttgtttg gcaacaggta taaattcaat tcccttttct ttttcaagtt
    12481 tcgaacaaaa tgcaatatca tattttcttt ctttaagacc ttgaataatg tcagtagtta
    12541 caccagtatg aaatttaaaa tctattgatt tagcaggatt agattttaaa aatttgtgca
    12601 ctatgtctgg gataaaatca gtaccaagtg ttctaagaaa agctaagtct atttgtcctt
    12661 caccagtacc agttattttt aatgatttta cacttgaatc taaaatttct aatgattttt
    12721 ccacatcttt taaaaatatt tttccatact tagtaaggac tatatttcta ccttcttttt
    12781 caaatagagc caccccaagt tcattttcta gtaaagaaat agcatgactt aagcttggtt
    12841 gtgttataga taaattttct gccgcttttg tatagtgttc taaatgtgcc aatgtaacaa
    12901 aatagcgtaa gtgatataaa ttcaattttt gttcatcctt tcataggtat ttttatttat
    12961 atatttgtag aagttaacct ttaagatatt tttacatttt tattatatat gattatagag
    13021 taagaataaa agcttttaaa aaaatttatc gttttttgtc tgaattataa gataaatata
    13081 tttatacgac ctaatagaaa aatacaagaa ttatcttata aataaaatct ataaacaaaa
    13141 tctatgaatt aataggaaat tatcaatttg ttattagtaa agtctcgtga tacaataaat
    13201 ttataaaatc tagataaata gaaatcaaaa aatactttta tttttaaggt aaaataatac
    13261 tactagtagg ataaataaaa atataaaatc aaaaatacta ttaattataa agaaaataaa
    13321 gggggcaaat ttaagctatg aataatcaaa aaaatcttaa tattccaatt acaattagtt
    13381 cttatacatt gggaacagaa gtttcattcc gtgatagagt tcgtattgca aaagaagctg
    13441 ggtttgatgg tattggattg cgtgctgaaa attatattga tgcaaaaaat gcaggtgtta
    13501 cagatgaaga aatgattgca attcttaatg aatatgatat gaaagttaca gaagtagaat
    13561 atataactca atggggaaga gaagaagata gaacggaagc tcaaagagaa aaagagcaaa
    13621 atgtttatca tatggctcac ttatttaatg ttaaacatat aaattgtgga ttattagaga
    13681 tgataccaga agaacaaatt ataactgcat tgggagaatt atgtgacaga gcaggtgaac
    13741 ttattatagg tcttgagttt atgccatata gtggtgtacc agatttggct actgcatggc
    13801 gtgttgtgaa aggatgtaat cgtgaaaatg caatgcttat attagatact tggcactggg
    13861 ctagagcaaa gcagacagca gaggaccttg cacctgttcc agcagataag atagtatcaa
    13921 ttcaagtgtg tgatgtttta gaaactcctt atgaaaaact tagggatgaa tcactacatg
    13981 accgtttggc tccaggtgaa ggatatggaa acacagagga atttgtgaaa ataataaaag
    14041 aacacaatat atcaccaaga gtcatagggg cagaagtaat atctgattct ctaatatcta
    14101 aagggttatc cactgctgct gaaactgtat ttaatgcaac taaaaaagtt ttggataaag
    14161 catggtctga agtatctcca aaataaactt atataatata aaaatataca gagtattata
    14221 atataaaact tgaaaggaag gaaaacctta tgaagattag ttctatgttt acacctataa
    14281 gaattggttc aatgacagtt ccaaatcgtt ttgtagttcc ccctatggga aacaattttg
    14341 caaacacaga tggtacttta agtgaaactt ctaaggctta ttatcttgag cgtgctttag
    14401 gtggttttgg attaataact atagaatcta gtgttgtaga caaaaaagca aaaggaggtc
    14461 ctcgtaagcc ttgtttatat gatgatagca caatagatag ttttaaaaat gtaatagatg
    14521 cttgtcatga tgcaggttcg aaggtatcta tacaattaca acatgctggt tctgaaggaa
    14581 atgaaaaagt agcaggacat ccattaaaag ctgcttctgc aatacctgca tcaaatggtc
    14641 gtaatacacc actagcaata acaacagaag aaatatatga acttatagaa tcttatggag
    14701 atgctgcatt acgtgcacaa aaagctggtg cagatgctgt tgaagttcac tgcgctcatg
    14761 gatatctggt tagtagtttt atatcacaaa gaacaaataa gcgtgtagat gagtttggtg
    14821 gatgttttga aaatagaatg agattacctc gtcttatcat tgaaaatata cgtaaaaaag
    14881 taggaaattc tctagcaata ttatgtcgta taaacagcac tgatgatgtt ccaggaggaa
    14941 ttgatgtaca cgatagttca gtaatagcag catatctaga agattgtgga atagatggac
    15001 ttcatgtttc acgtagtata catatacatg atgaatatat gtgggctcct acgactttac
    15061 atgctggatt cagtgcagaa ttagttactg aaataaaaaa agctgttagt ataccagtaa
    15121 taacagttgg acgttataca gagccacagt ttgctgagtt aatggtcaga caaggaagat
    15181 gtgatttagt agcattcggt agacaaagct tggctgaccc agaaatgcct aataaagcta
    15241 aaaatggaaa actagacgaa atgataccat gtattgcatg cttacaagga tgtgtaccaa
    15301 atatgttcca aggcaaacct atagcctgtc ttgcaaatcc tatattagga catgaagcag
    15361 agcttaagcc agctgaaatt agcaaagaag ttcttgttgt tggtggaggt gttggaggta
    15421 tgcttgcagc atgggtatgt gctaaaagag gacataatgt aactttagtt gaaaaatctg
    15481 aagttcttgg tgggcaaatg cgtttagctg catatcctcc agggaaagga gatattacta
    15541 acttagttag aagttatatt tcaaaatgta atcaatatgg tgtaaaaata tgtactaata
    15601 cagaagctac agtagaactt ataaaggaga aatcacctga tgtagttatt atagctactg
    15661 gagcaactcc acttgtactt cctataccag gaatcaatga ttcaggatta attcatgctg
    15721 tagaccttct tgatggtaaa aaatcttgtg ggaaaaaagt acttgttgtt ggaggtggaa
    15781 tggttggatg tgaagtggca gccttccttg gagaacaaga acatgaggtt actgttattg
    15841 agcttagaga agaagttggt gcagatgtaa tttcagaaca ccgtaaattc cttatgaatg
    15901 attttgaaaa ttataatata cataccatta ctggtgcaaa agtttctaaa ttttatgatg
    15961 atggtgtcga ctatttactt acagatggca cagagcatga tttaaaaggt tttgattcag
    16021 tagtacttgc tatgggttct cgtaattacg acccattaag tgaagttata aaagaagttg
    16081 taaaagaaac ttatattgtt ggtgatgcag taaaagctcg tagggcatta gatgcaacaa
    16141 aagaagcttt tgaagtagca ttaaatatat aaataataaa taaatttttc tttgtaaata
    16201 actgaatagg ttatttgcaa agaaaatgat aatattgaga ggagattata gttatggaaa
    16261 tttcaggtag aacaggatta tttgcattaa taggtacacc ggttggtcac tctaaatcac
    16321 cagtgatgta taattatagt tttaagaaat tagatttaga ttatagatat ttagcctttg
    16381 atatcacagt agataaggta aaagaggcac ttttagcaat aaaaactttt aatataaaag
    16441 gagccaatgt tactatgcca tgtaaatctg ctgttacaga atatatggat gaactttctc
    16501 cagcagctcg tataattgga gcatgtaata caattgtaaa tgacaatgga aagttagttg
    16561 gtcatataac agatggagta ggctatgttc gtaatttaaa agaaaatggg gtagaagtta
    16621 aaggtaaaaa gattacaatt atgggtgctg gtggtgcagc aactgctata caagtacaat
    16681 gtgctttaga tggagcaaga gaaatatcta tctttaatcc taaggatgat ttttataaaa
    16741 gagcagagca aacagttgag aatataaaaa aagatgtacc agaatgtgtt gttaatctat
    16801 atgatttaga agatacaaac aaactttatg aggagataca atcaagtgat atattgacaa
    16861 atgcaactct tattggtatg aagccttatg acaatgaaac aaatataaaa gatactagtg
    16921 ttttaagaaa agatttagta gtaacagatg tcgtttataa tcctaagaaa acaaaaatga
    16981 ttgaagatgc tgaagctaat ggatgtaagg ctattggtgg attaggaatg ttattatacc
    17041 aaggagcaga agcttttaat ctatatactg gattagaaat gcctgtagaa gaagttaatg
    17101 aattatgctt taaatagata agagtaatat cttaaacaat gaaaaaacat acgtatttca
    17161 atatttttaa taataaatta caaattgtca tagtgggttt tgcctcatag tccagttaaa
    17221 tttgaagttt taactgacta tactttaaat ccattatggg gatgagttta ttgtacacaa
    17281 aaatgttaat aaattaacaa gaatgggaga aaaattatga acaataaaaa atattatcct
    17341 actgccatag cgttgtattt ttcatatttt ttacttggta ttggaatttc aattttagga
    17401 caatacaaac cagaattttc atcaatgtgg ggggctaaaa cattatcaga tggaacattg
    17461 gatgtaagta ttgttttagc tgttattgct gcactaggat tagggagact tatatcttat
    17521 ccatttgcag gacctatttc agataagtat ggaagaaaag taagtggtct tataggaaac
    17581 tttctacatg caattttctt tgttggaatt gtattttcac caaattttta tatagcatat
    17641 gtatttgcaa taattggagg ggcagcaaat tcattcttag atacctgtgt aactccttct
    17701 tgtatggaga tatttgctag tttaggaaca atagcaaata tgtttactaa atttacaata
    17761 gctttagcac aatttttgct tccttttata attggatttg ttgcagcaaa ttcaatatca
    17821 tttaaagtta tttttatagt aacagcgatt cttattgtag tagatgcctt tttgatagct
    17881 gtgttaccat ttccaccagc taataatgtt attgataaca aaggcaaaac agttaaatca
    17941 gagaagatga aatttacacc aacaagtata gctcttgttt gtattggatt tacatgtaca
    18001 tctacatttg tattatggtt aaattgtaat caggaattgg gtaaattgta tgggatggct
    18061 gaccctacaa aaattcaatc cttttactca atgggagtaa tatgtgcagt attaataact
    18121 tctttactta ttaaaaaata tattaaacca ataagaatat tagttatata tcctataata
    18181 gctttactta tgctattagt tgtatatttt gttcaaactc caactatttg tatgttaggg
    18241 ggatttgtaa taggttattc agcagcagga ggtgttcttc agttaacaac gtcaactgca
    18301 aatgaaatgt ttccaactaa taaaggaaaa ataacatcta tagttatgat tgcatctagt
    18361 atagcaaact atgttatttt aaatatagca ggtattatta caaaatcagg gggagttaat
    18421 ggaccaaaat atgttgtatt gtttaatgtg gcaataacat ttgttggaat tttacttgca
    18481 ttgtttgtaa acatgagata tgaaaaagaa aaagtgtatg attatgatgt ctaatattag
    18541 tataatatat gcatagagaa gtctaaatct aggatagatt tttattaaaa gtaattaaaa
    18601 ttaataagga tattatttac agttataaga tagggtattt acataattaa taataaggat
    18661 attactagaa tttcaatttc tagtaatatt tttttgcgta taaatgtaaa cttgatgaaa
    18721 taagtagtat aatcaatata aattacatca tgtaaaggag gataatttgg tggagaatgt
    18781 ttttaagcga ttgcaggaat ttaatggtta tgatggatac aaagaatcat ttgaaatgaa
    18841 ttacctatgt atatatgaaa gtataccgtt aagagaacaa gtagagcttg caaataatct
    18901 agtagatgaa attttaaata tgtataaaag tgaatctaat gaaatttatt tattggaggg
    18961 tagtaattct aagtctttaa tttgttattt cgaaattttt atgaaaaaga taaatacttt
    19021 ggtaaaagaa atgattattg atgagaaatg gttatataaa ttaactaaag aattaatata
    19081 taagagtaaa aaagttgagt atgtaaaact agggttagtt ttaagtgaga aatatctaaa
    19141 tgtagaaaac ttaagagaag ttgtagatac attttctaaa tctggagaat acgtatttta
    19201 tctgagtaat actattaaaa aacttgaatt ttacaataca tatttattta atttatctaa
    19261 aaaggctaca ggttcaataa aagtatttgc tattgttaat atggaaaatt tagattctaa
    19321 aataaattcc tatctaattg aagatggtta taaggataca aagtacgaac gattattgat
    19381 gaactatatt atttcaattg tagatttgaa tgaatatctt gaaaaacgag atttagataa
    19441 agaaaagata aataatttag cacgtcttat atgcaattac ttgctaagtg tagaatttaa
    19501 atatataggt aataaattag aattagtgaa tcgattttta cctactgtag taaattatgg
    19561 gactaacttt gaaagtttat attcaatttt cctaatagct ataaacgtct taaaagatga
    19621 aaatatagag tgcaataaga ttgaatttga aaaagaaata aatggtatct tgctcagtga
    19681 aaaatggaaa aatatatatt ttgaagcatt aagagatgct agtggaaaaa cagaagatat
    19741 tataaaaatg tcagaaattt atgatgtaaa cttatctttt gatgacttgt tgccatatct
    19801 aaatagagat attagagatt ttgaggtata ttggcatata tctaaaaagg gaacgacaag
    19861 ttctagatta aaattattaa atttttttga agagacgttt aaaatagatg atttaattgg
    19921 aaaaatgaaa gatatagaaa aagataaatt gactcaagaa tattatgatg atatgttatt
    19981 ttttattgtg cttaaaggaa gtaaatcact atatcctgaa ggaaagaata tatctttaaa
    20041 aggaatattt ggaaatataa atgaagtaag aaaagaatcc ataaatatct taaaaagata
    20101 cagagagaaa ctatctcttg aagaattaaa aattgttaaa gaagcgtatg aaaaagaaaa
    20161 aaatgttata ttgaaggatg aattaagaag agtattatat gaaagtaata atttaaaaaa
    20221 agaatttgta aatattgaaa aaataaaagt agatgaacat ggtaaggata tctatttgac
    20281 ttcaatagct gttgctggtt caaggtttag aaatagagaa tatcttgaaa aagaattgga
    20341 gaaaagtaag atatactatt taactagaga gaaagataat ctatatgatg aaaaagctat
    20401 taaaatagtt ggtgaaactg gatatgtgat aggctatgtc ccaagaaaag aaaactatat
    20461 tttgagcaat ttattagatg gaggaaaact gctgtattgt agagtaactg aatataattt
    20521 atatgaagat tgtatctacg caaatgtgta cttgagttat aaagatgtaa ttgaaacagt
    20581 tgaaaactct ttaaagatgg ttttagataa gagtagaata aaacttataa actaagacgt
    20641 ttatatattg cttagatgat gtaatcctaa ctgattggtt attttttctg gaattatggt
    20701 gtattttatg agcaatgcta atgaatataa aataaaaaaa caattgtttt gtatatatga
    20761 tgtatataaa tatatttata tattcatatt tacaaaacaa ttgaaattta tgttatttta
    20821 taattttaaa tttagatgga attttaactt ctgctttatc ttttatttta gaagcttgtt
    20881 ttttaacttt atttaataat tcagattcat ctacaactaa tacttttctt tccttcatca
    20941 accatttacc atcacaaata gtagagtcta cattttcact agtcatagaa taaactatat
    21001 ttccaattgg attatgcatt ggtaaacaat gaattgtatc tggatttaga ataatcatat
    21061 cagctttttt tcctacttca agactaccaa tttctttttc tagtaaagca cattttgctc
    21121 catttatagt agccatttct aagacttctt cagctggtac gacagttggg tttaaagttc
    21181 ttcctttatg tattagtgat gtaagataca tgtccctcat aaggtccatt ctattatttg
    21241 aaggagctcc atcagtacca attgaaacag gtattccttt ttctaacatt tcaggaattg
    21301 atgcaaaccc tagaaccact ttcatagctg aacctgggtc atgagaaact tttacatcat
    21361 gtaatctaaa taaatcaatt tccttatgag taagccaaac tgtatgagca gctaaaagat
    21421 ttggtcctaa tacacctagt ttatcaaggt attcaactgt tccaactcca ttgttttgct
    21481 ttacaaatga aatttcatca gctatttcag caatatgcat atgtatacct gtattgtatt
    21541 tatcagctag ttctttagtt ctaacaagta attcttcagt tgtattaaaa atagttctta
    21601 atgcaaacca tattttaatt ctctcattag atgtattatt aaacttatgg aataagtcta
    21661 attgagtgtt gatttcctca tcagtagttt tttgccatgc ctcaggaaga ccatctcctg
    21721 tatccataac tgatttagct aaacaggctc taagtccagt tttatcaaca gcttcaacca
    21781 tagcatctac atattgacca ccagattcta aaaatgtagt gacaccagat tttatcaatt
    21841 caacacaaca tgcagtagat gaaatcaagg aatcttcata attgaagcta ctttcgtatg
    21901 gccaaattct ttctctaagc caagttaaaa gtacaacatc atctgcaaga ccacgtccta
    21961 attgttgaga taagtgaaca tgagtattga ctagccctgg cattagtatt tttcctttag
    22021 catcatatat ctctgcatct ttatctacaa tattaatatc aaacttacca acctctttta
    22081 taatattatt ttcaataagt atgcttccat tttcatatac ctctctatct gcattcatag
    22141 taattagata tgcatttttt ataagtaatt gtgacataaa caccactcct tttctatata
    22201 tttttatatc tatatattat gataataaat tttctataca aagttaatag caaaatcaat
    22261 ttacatataa agtgattttg ctatttataa atttaaaata aataagattt tattctatat
    22321 aatttaaaat attgacctag aactatttaa aaatattaac ttagaactaa gtttacaact
    22381 gcaagtaaac ctataatcca aagtgtaata ctgattttat cagtttcttt agctactaca
    22441 tgcattatta tatatgaaat aaatccaaag cataaaccaa tacttatact ataagttaaa
    22501 ggcatcaata aaactgtaac aaaagcaggt acagctattt tgctatcagc aaaatcaatc
    22561 tgttttatgc tttcaaacat gaatacacca accattacaa gtgcaggagc tgcagcgtaa
    22621 gtaggaacta taccaagtaa tggtgtaaat ataagtgtag ctaagaataa tgctgctgtt
    22681 actattgaag caagtcctgt tcttgcacct gctgcaatac cagaagcaga ttctccaaat
    22741 gttgtaacag tactagttcc taagaatgag cctattaaag tagaagaaac atctgcatat
    22801 aacattcttc ctaaatcttt tatttcacca ttctcatctt caagacccat ttgtttacaa
    22861 caagtaataa gaaatgctaa tgaatcaaac atgtctataa acatgaaaga gaatataggt
    22921 ccaattaatg agaatttcaa tgcacctaca atatctaatt taaaagcaac tggtgctata
    22981 ctaggaggtg tcgagattat tgctgttggc aactccacaa atccaaatat aacagatatt
    23041 attgttgcaa ctactatact tatcaatatt ccacctttaa cttttttata ttctagtatt
    23101 gccattaaga taagagcaaa aagagctatt aaaactgttg gtgtaaaatc tgcaagtgaa
    23161 actatagtat ctggattagc tattataaca cccatatttt ttaaaccaac aaaagcaagg
    23221 aaaagaccta tacctgctga agatgctact gccaattctc taggaattgc gtctgcaatt
    23281 gtctttcgaa gacctgttac tgcgagtgta aagaagacac atccagatag aaaaactacc
    23341 ccaagacctg tttgccatgg gattcctgca cctataacca aagagtatgt gaagaatgca
    23401 tttaatccca taccaggagc tagagcaaat ggtgcatttc ctaaaatcgc aaatataagt
    23461 gtaccaatgg ctgtagctag tatggtaact gttataaggg cggctttatc cattccagct
    23521 tcagataata ttgctggatt tacgaaaact atgtatgcca ttgttaaaaa tgtagtaact
    23581 ccacctatta cctctgtttt aaggctagag ccacgttcgc taaatttaaa cattttgtct
    23641 aaaaagctta atgatgaatt atttccacct ggttgtacat tgttagtgtt tggtaccatt
    23701 tttacctcca aaaaacttta agatataaac aaaaaaatat atattcatat agatagaata
    23761 gaacatatat tcagagttga cattctattc atagaaaacc catttaaggt gagtttgtag
    23821 aaactattag accgtattac taatattata tgaacatttt ctatttgttt ttctgttgta
    23881 ttatatcgta atattgtatt atttacaata aatataataa aattattcgt ttttttgtcg
    23941 attttataaa aaattaaggt tatttattga aaaaaattac tttttccaat aattgaatat
    24001 gtatgaatat aatgtcaatt attctaactt gttttatact attaatggaa gtttattata
    24061 aatgagtttg tgatataatt ataaaagtac tgaatacatg gatatttcgt aaaaactttt
    24121 taatatatca aatatgtata ttaagttaat atattaaaaa aaataatatc aaaaataata
    24181 taatttatag tggaggggaa aatgactaaa attattaaag tgcaaaaacc taaatttaca
    24241 ggagaattag aaattattga aaatcttgaa gatatacttg aagatatatt taatgatgaa
    24301 aaaatattta ataaaattat agaaaatgaa gttataagtg atttagattc tgtaagagtt
    24361 agctttgaat cttgtatatt tagaaatgtg tcttttgaaa attgtaattt aaaaaagatt
    24421 gatttacttg atgtggtatt tgaaaactgc aatttatcaa atacattttt tgatggagga
    24481 agtatataca gagttgaatt taaaaatagt aaattgatag gagttagatt tgatgattgt
    24541 atacttaaaa acgttttgtt caaggatgtt ttaggtaagt attctaactt ttcattctct
    24601 aaatttaaga tagtttctat cttagattca aattttgaaa attctgtatt tcaagaagta
    24661 aaaggtgaca atctagtatt tgagtataca aatttaaaaa aagcaaactt taatggaact
    24721 aaactttctg gcatagattt tactactaat aatattgagg gaattgagat tggtatagat
    24781 gatattagag gagcaatttt tgatgtgtca caagcaattg atttaacgaa attgatggga
    24841 attatcataa aatgaaaaaa atattcataa aataaattgg gaaaacgtta acaatgtgtg
    24901 aatttttacc agattgacat ttttctgaaa ataaagtaag attatcttaa tattcaaaat
    24961 attttcaaag ggggtaattt agtgtgagta agcagatgag agatattttt atagttggtt
    25021 ttgctttatt ttctatgttc tttggtgcag gaaatcttat gtttcctcca tttttaggga
    25081 tggaatccgg caaagactgg ttaattcctc ttattggatt tgttgttgca gatgctggta
    25141 tagcaatgct tgttattata gcaacagcaa gatgtaaggg aagcatggat gatgtactta
    25201 ggagagctgg taaagggtta gcaagagtaa tgagtgtagc tgctctagca tgtttagcac
    25261 ttcttgtaat accaagaact tgcgcaacaa catatgaaat gggtattatg cctatatttg
    25321 gagaccatgg tccagtagct aaagctgtat tttcaataat cttctttggg ctaactttag
    25381 catttactat taggtcttct aaggttatag acataatagg taagtattta actccagcgt
    25441 tattattagt attatttgtt ttaatagtaa agggtataat ttctccagca ggacctatga
    25501 gtcctgagca tatgatagat aaaaatctat ttggtgaagg tatatcacaa ggttatcaaa
    25561 caatggacgc tctaggtgca gcatctatgg caactataat gttgatgtct ataatagcta
    25621 aaggatatac atcagataaa gaccaaatag gaatgactat taaagctggt tttgtagcct
    25681 gtgtatttct agcagtagta tatggtggtc ttacttattt aggtgcaaca gtttcaactt
    25741 tatatgatac tagtatacct caagcatctt tattagttga aataactagt atattgttat
    25801 atggtcaagt aggaaaggta attttaggtg taattgtggc acttgcctgt ctgactactt
    25861 ctacaggttt aacagcatca atatcaactt attttgaagg aataagtaag gcgagttata
    25921 aacaactggt tataggtata tgtgtagcta gtatgcttat atcaaattta ggtgttgatt
    25981 ctataatagc catatcagtt ccaatcctac aaactatata tcctgtttta ttagcaatag
    26041 ttgttatgga gttggctggt aaacatataa agaatgataa tgcttttaaa ggtgcagcat
    26101 atgttacatt agtgattagt ttattatcag ctataaatgg aatgacagga gctgttccat
    26161 ttatacaaag cttgccactt gcaggtttgg gctttaactg ggtaatacca gctatattgg
    26221 gtggaatagt tggtaacttt ataaaaagca acaaacaagt agcataataa tttgtaatta
    26281 atttaatttt ataatatatt aatttttagt actatgggta ggtttaatct aaacctaatc
    26341 tacccatagt actttttaga ttggattata atagcatgta tataattttt tataatataa
    26401 tttttctttg aagtatgata aaattagtaa tagtgataag aatgtacaaa tttaggaggt
    26461 tttaattaat gcttagaaaa ataacagcat ttatgctatc catatgttta attattggac
    26521 ttacaggttg tagtttactt gtaggcaaga aagataaaga gaaaattata gatgatttta
    26581 aagttgcagt ggtaactcaa ccattatctg aaaataaagt acaatacaat atggtggaag
    26641 agatggcaaa agaatatgag gaagagaata aaattgataa agacaaagat ggtcaaacta
    26701 aagttaaaca aactataaag catgttgtat tgccagagaa ttttacttct aatatagaca
    26761 gtgctattaa taagatagta aagttggctg acgataaaga agttcaggct attgtagttt
    26821 caactgacca agcagggctt ttacctgctt tacaaaaggt aaaggaaaag cgtccagaaa
    26881 taataactat ttctgcccct atgggagatg ataaaaacca attaagccaa tttgttgatg
    26941 tgaatctagg ggttagtgca gaggaaagag gaaaagtatt agctgaacgt tcaaaagaaa
    27001 tgggagcaaa agcttttatt cattatgcct ctacagatga tttgaaagat gttaatatag
    27061 ccaaaagatt agaaatgatt aaagagactt gcaaaaatat aggattgcca tttgtacaag
    27121 ttaatactcc aaatataaat actgaagaag acaaaaacaa agttaagcag tttttaaatg
    27181 aagatattga aaaacaagtt aaaaaatatg gaaaagatat aaatgtattt ggtgtaaatg
    27241 agtatatgga tgaagttata ttgactaagg ctttagaatt aaagtatata gttgcagagc
    27301 aaagcaatcc atcaccaata caaacatatc ctagtgttat gggtctaaaa atatctgaaa
    27361 aagatgctca aaattatgat aaaataaacg acatgataag tgaaaaggct aaggcttttg
    27421 gcatgtcaaa tagacttggt ggatatccta tgccaatgga tgcttttcta ccatcagtag
    27481 caatttatct tgctactgaa atggtgaaac aagatttaac acaagaagat gtatgtgacc
    27541 cagattattt agaggcgttt acagaattaa gatttggaat cggttcagaa tttactcctt
    27601 taacagaggt actttataat tatcaatcag taattttaag ccaattaatt tattaatatt
    27661 aaggaagtgt ctcaaaatga actttttagt ttatgaggca ctctttttct attaaatcaa
    27721 atatattttt atctttttaa caattaaaaa gaagcttatc cctatattga gatagcttcg
    27781 tttttaatat aaataagttt tattatttta taaaaaattt atagaaaaat aaaaaattat
    27841 aattatatat aaattttgat aaagtaaaga tgaattacta taggtttaaa ttatattatt
    27901 ttctattatt tttatgcctt tctcagttat tgatgagcct tttcttcctt tactagaaat
    27961 taagtatcca ttgcgtgtca aatattctag catatttctt acttcgtatt ctgtgacatt
    28021 atattccata aatttcatta actttgtcct ccccattggt ttcatgtgca atgattttag
    28081 gatagaaatg catatgtttt tgtcatattg gtttaaatca ttgaatatgg acatattact
    28141 tgacatatga ttattattta attttgaaga tatgttttga ggtaaatcat ttattgtaag
    28201 ataattggaa cccatgtaag ccatatattc aatacaattt ctcagttctc taatattccc
    28261 catccaagtg tatgaattta aagcttctaa aagactctta tcgagtttta tgtttttata
    28321 gggaagttct tttaaaaatg attctaaaag aagaggtata tcttctaatc tctttctaag
    28381 aggaggtaaa tctaaagtga acatacttat cctataaaat aaatccatcc taaatttatt
    28441 ttctataatt aatttctcta aatctttatt tgtggcagct aatattctca catcaattgg
    28501 aataatactt gtaccaccaa tttttctaat ttctttttct tgtaagactc ttaaaagttt
    28561 aacctgtaaa tcatgtggca tatctcctat ctcatctaaa aatatagtgc cagtgtgagc
    28621 taattcgaat aatccttttt ttccaccttt ttttgctcca gtaaaagaac catcttcata
    28681 tccaaatagt tcactttcaa gtaattcact tggtaatgaa gcacaattta ttgctacaaa
    28741 aggtttattt tttctatttg aatgattgtg tattgactga gtaaaggctt ctttacctgt
    28801 tcctgtttca cctgttatta atataggatt atctgtaaga gccatttttt ttgccttttt
    28861 aatacaatct tttataatag aactatttcc aataatatta ttaaatttat atttagctac
    28921 ataacctctt ttgacagaat cacttcttat tttttgttct aatatttcta tattgtttac
    28981 atctttcaat gtaattaagc aaccctctat atttttataa agataaaatg gttttttaga
    29041 aagtatgaat tttttatcta tttcgtctat gtatataatc atattatcga tatttaagtt
    29101 ctttgtaatt ttatctaaga aactatttgg catatattcc attaaggaag gtgattttat
    29161 aagtttttca tctagtccca acatattaag taaagactta tttacaaaag taaccttgtt
    29221 aaaggtgttg aaaaatatta gaccatcacc tatttcatca atggtcatgt aaagtatagt
    29281 ttttaattta gatatattat ctaatgatgt atttaagaaa tcatgactta gtgtctcttt
    29341 ggacaatttg tatagttttt ctataaattt ctcatttttt agttttaata ctactaataa
    29401 tgacatatat gtatctaatg agatttttct ccaacctaaa tcaacaatat tttttatgta
    29461 ttttggtata aatctaatct gacctggtgt tatagctgta tctaaatcat atatttcatc
    29521 acaatctgga tatgtcggta caaaatctat atgatttata ccaattttat atagataagt
    29581 agccatctca aatgctgaag ttttattggt tgtcataagt aacgattttg tacctggagg
    29641 tagtttcatc attttttcat atccaagttt tgaaagtgct ttcgttccat gaataatttc
    29701 aatatcttcc ttaaaatgtt ttattaaaaa attactagtt aaaggtgata gagaaagcac
    29761 tacatctccc tctaaatttt cttttattcc atcaagtata ttttgtgttt ctatgattat
    29821 ttcgtctcca aaaaagtttg ttaattcttc tttgtagtgg tcactattca atttatcatg
    29881 tgttataagg attaacttct ttttatccat tttatttctc ctaacaaaat agtatttatt
    29941 tttaaagtat ttaaagtata aggcaagtta tattatacaa caaatagtag tataaattta
    30001 tatatttgat taaataatag gtttttttag gattttatcc tataaaaacc tattttaaaa
    30061 ttatttttgc aagataagga aaatagcttt ttatattgta atgtatattt taaataatta
    30121 ataaaaaaat attatatttt taaacttgaa aagtaagtaa ttataagcta tttaatcact
    30181 ggaataaatt tacaataaaa aaataaatac aaaggcttta tcattaaggt attggcataa
    30241 gttttgctta ttaaacaagt atactaataa aattcattta aacaggaggg agaatttata
    30301 tgagtaaatt agaatcaaca acagaaaaag ttgaggtaaa atggtatggg tatgtagcat
    30361 taatattggg agcattattt ttttcaggaa tatttaaaga tgcaccagga ccattaaaag
    30421 tattggattt taacaatgta ttaggttctt ttggaacatt gggaacaatt aatgatggtg
    30481 taggtacatt agctgcaaac tttagaggtg atggaggtac tggccctcga gatggatggc
    30541 tatatgcatt gacattaata ccatcagtta tgtttgcact tggagtagtt agagttatag
    30601 accacttaga tggaatgaag gcagctcaaa agcttcttag tccactatta aagccattat
    30661 tagggttacc tggatttgca ggactaactt tgatagctag tttacagtct acagatgcag
    30721 ctgcatctat gactaaagaa cttaaagatg atgggtatat agatgaaaaa caaaaagctg
    30781 tattttgtgc atttcaattt tcaggtgcgt ctgcaattac gaatttcttt gcttctggag
    30841 cagcactatt tccatttata ggagatgtac caatatttat accacttgct ttaatattaa
    30901 ttatgaagtt tgttggggcg aacttactta gattatattt aaataaattt gacaaggggg
    30961 aagcttaaca tgagtgaggt agcaaaaaaa gaagtaaaag ttaaaaaagg aaatgtgata
    31021 gatgcgttta ttggaggagc tagaaatgga ttccaaataa gtacaaattc aatggctcca
    31081 aatgtcatct tcggttttgc tataatttct gtgttcaatt taacaggtct tttggatgta
    31141 atagggacaa tatttacacc tgtaatgagt atctttggac ttccaggtgt tgcagcaaca
    31201 gctataatga caatatttat atcaatggga ggagcatctg gagtaatcgc aggtttattt
    31261 acagcaggtc aaataagtca agcacatgtt gcaattctat tgccaatgtt attgctttca
    31321 ggagcgcaga tacaatttat ggggagactt ttaggaacag ctgagttaaa aactaaatat
    31381 tatccacata tgtttgttat agcaacttta aatggttgta ttgcaatgct tataatgaat
    31441 tatttcgtag tataggtaat tataataaag atttaatata aatggaaagt ttaaatttta
    31501 aggaggagct agattatgga aactaacaaa gactatattg agtatgtaca taaagtgtat
    31561 gattatctac acactatacc agaaaaagga tttgaagaat ataagacatc agagtttgtt
    31621 ttaaaagagc ttgaaaaaat ggggtttgaa aatattaata aaggaattgc aggaactggt
    31681 attatagtaa ctatagattc taaaaaagaa ggaccagtat ttgctataag agcagatatg
    31741 gatgcattgg aatttatcat agatggagaa aagaaaatga tacatgcttg tggtcatgat
    31801 ggtcatactt ctatgctttt aacagctgca aagttaataa aagagagaaa tttagtaaaa
    31861 agaggtaagt taaaaatact ttttcaacca ggtgaagaaa aattatatgg agcattaaga
    31921 gtaattgatt ctggtttgtt ggatgatgta gaagaaatgg tagggatgca tttaagacct
    31981 ggacaagatt gtaggttggg aaaggcgata cctgcaatgt accattgtgc tagctgtata
    32041 atggaattta cgattaaagg tatcacttgt catggttcta aaccacattt aggagtaaat
    32101 gcagtagatg ctgtctgttt agcagtgaat gcaataaatg caataagagt aaatccagct
    32161 ataccaaatt ctataaaagt aacaaatata tcagttaagg gagaaactta taataatata
    32221 ccagatgaag catttatggc tttagatata agggcagaaa gtaatgatat aatagaagaa
    32281 ttaaaagaaa aagcaataca tgctgtaaaa actgctgtaa gttctataaa tgcagaagct
    32341 gtactaattt ccaaagatgg agtaccagca gcagaatttg atgaagaact tgtaaacttg
    32401 gctgaagaaa ctataaaaga tgtattggga gctgatgcat ctataggtat atttaaaaat
    32461 tctggtggag aagatttcca ttattataca caaaaactaa aatgtaaaac tacatatatt
    32521 ggattaggag ctgatgctac accaggattt cataatccaa atgtaacatt tgatacgaaa
    32581 gctttagaat atggtgtaga tatatggtgt agattggttg aaaagagatt gggataacat
    32641 ccaaactaat aattctacat ttaaaattat aattatagaa gttatagaaa aagattgctt
    32701 atgttaatta gaaaataatt aatatgagca atcttatttt gatataatga aactgatttt
    32761 ggaatattga aaaaatatga tggaaaagta attatatttt aaatctattg gacaaaaaag
    32821 tgatattatt ataatattaa gaattgtaaa ataataccaa agtgcatcta gtaatagact
    32881 taacaattat aaaaatttta ttcttaggag ggaaaaaatg agtaagtata attttgacag
    32941 agtaatagat agagtaggaa cagattgtgt aaaatgggat tttagaacta attgttcaac
    33001 aaaagcacaa aaagatggtt tgcctttttg gattgctgat atggattttg aatgtgcaga
    33061 accaataata gaagcattac gtaaaaaagt tgaacataaa atatttggat atagttcaaa
    33121 tgatagtgat aaatacttta atgcagtttg tgattggtat aaaaggagat ttaactggga
    33181 aataaataga aaagatatta tttttagtcc tggtgttgta cctgctgttg ctattttagt
    33241 aagaatatta actaattcta atgaaggtgt aatagtccaa aaaccagtgt attacccatt
    33301 tgaagctaag ataaagagca ataataggga tgttgtaaac aatcctctga tatatgagaa
    33361 tggtacttat aggatggatt atgatgattt agaagaaaaa gctaagtgta gcaacaataa
    33421 agtactgata ctttgtagtc ctcacaaccc tgttggaaga gtttggagag aagatgaatt
    33481 aaaaaaggtt gtagaaatat gtaaaaaata tgacctatgg ataatagctg atgagataca
    33541 ttctgattta attagaaaag gatttaaaca tactccttta cagtcattat gcccagagta
    33601 caaggataaa atagttacat gtatagctcc aagtaaaaca tttaatctgg caggaatgca
    33661 gttgtctaat ataatagtga ataatgatga actaaaaaag aaatatcaag aagaggttac
    33721 agctgttgga gttgcaactt caccaaatcc ctttgctatt gtagctacaa tagcagcata
    33781 taatgaaagt gaagactggc ttaatgaatt aaatgattac ttagataata atatacaatt
    33841 tattgatgaa tacttaaaag aaaacctacc aaaagtaaaa cttgtttatc cagaaggaac
    33901 atatctagca tggttagatt ttactgctta tggattaaat gaagtagaac ttgaagattt
    33961 aatgtttaaa aaagctaatg tattatttga tgaaggttat atatttggaa aagaaggaat
    34021 aggatttgaa agaataaatg tagcttgtcc acagtcttta ttaaaagaat gtatggacag
    34081 attaaagact atatttgaaa atttataaag tataggtaaa aatttataaa gtgtttaaaa
    34141 ataaaaaaat aataaacttg acaaatttta actaatcact tatcatacta ttatgaataa
    34201 atagaaaaca taagagagct aaattttatt gaattaaact cttaaagact ttgaaaggaa
    34261 gagtagttat agaatgtaat taaagcgagt tggtgatggt gtgagaccaa tatgaagtct
    34321 ataatgaaga acatcctgga gttgctaacc gaaattttaa aagagtaggc ttagtcggaa
    34381 ccaaaccgtt atcatattgg ggtgtatcga gtaatatctg tacattgtga gctgggtttg
    34441 aatttatttc aaactaatca gggtggtacc gcggaaaagc aacctttcgt cccttttata
    34501 tagggatgaa gggttttttt aatggaaaaa tatataaata tagggagtga atgatatgag
    34561 cacaataata ccaaaggatt ataaaagtag tttgaacgta attgatacac aaatagctat
    34621 aaaaaaatta aaagattttt ttgaaatgag actaagtaat gaattaaatc ttataagagt
    34681 ttcttctccg ttatttgtat tgccagaaac aggtgcaaat gataatttaa atggagaaaa
    34741 ggctgtaagc tttgatatac catttatgaa taaaaatgca gaaattgttc aatcacttgc
    34801 aaaatggaaa agattagccc ttaaaaaata tgggtttgaa gttggtagtg gtttatatac
    34861 agatatgaat gcaataagaa aaaatgaaga attagacaat atacattctc tttatgtaga
    34921 ccaatgggat tgggaattgg ttatagataa aagtagtaga aatgaaaaga ctctaaaaga
    34981 tgttgtaaaa agattgtatg gagtatttaa agatactgaa atttttgtat gtagtatgta
    35041 tgaaggtata aaagaaatat taccagaaga aataactttt ataacatcac aagagttaga
    35101 agatatgtat ccagaattat caccaaagga aagagaagat aaaattgtaa aagagaagaa
    35161 agcagtattt ataactcaaa taggaaaaac attaatatca ggagaaaaac atgatggaag
    35221 aagccctgat tatgatgatt gggaattgaa tggggatata ttattttaca atcctgtttt
    35281 agattcagca ttagaattat cttcaatggg aattagagtt gatgaagaat ctcttgatag
    35341 acaattgaaa cttgctggat gtgaagagag aaaagagttt gactatcata agatgctttt
    35401 aaatggcgaa cttccgtata caattggtgg aggaattggt cagtcaagaa tatgtatgta
    35461 tttcttacaa aaagctcata taggagaagt acaagttgga gtatggccac aggatatgat
    35521 tcaaaattgt agtagtgcag gaattgaatt attataattt aaatataaac taattgataa
    35581 aatttatatc taatatgttc atcaaattaa ttatatcggt tataatgtag aataacctat
    35641 aattataaga ggtgataaat atggagtttt taaaaataat cattaatata gttttagata
    35701 ttttaaaaaa aattttggtc agatttaaaa atgctaaatt tggtttggtt tttgtatttg
    35761 atttacttaa actacctgat tttatgacag ataaaagaat caatatagtt gataaaataa
    35821 aggtaatttc tgtattaatt tttactatat cctactttgt atctggggtt gatataattc
    35881 cagagatgat tgcaggggca tttggtttta tagatgatgc tatagtttta atttggagta
    35941 taggtatagt gaatgaagaa attaataagt atagagttat cgcaaaaaaa gataaacatt
    36001 caaatataat tgaaaatgta gaattttcta taaaagatga agaagaataa caatttatat
    36061 atgttattct tttttttatt aacaaaaaat ggttaaattg ttgacggaga ggtaaaactc
    36121 tattatcata ataagtggat atccaatatt taactaagta ttcaatgtgc aagaatgctt
    36181 aatttaaaaa agttatggag ggtaaatatt atgaagaaaa aaataggttt agtgcccaaa
    36241 ctaatcatcg gtatcatagt cggtatttta atcggaagtt atgcaccaga aataatagta
    36301 caaatattag taacagttag tactttattc tcagcattcc taaaatttgt tataccattt
    36361 ataattatag gttttgtaac agcaggtata gctgatttag caacaggtgc aggaaagtta
    36421 ttggggataa ctacgggaat agcatatggt tctaccttaa tagcaggatt attatcattt
    36481 gcagtatcta cattaatctt cccaaatttt attgatgcaa gtgttgcaag tcaaattgga
    36541 gacccagagg caggaatgtt agctccaata ttcacaattc cattagagcc tatggtagat
    36601 gtaacagctg caatcgtatt tgcatttgta atgggacttg gaatatcagc tttaagaaat
    36661 catggaaaag gagagacttt atttaatttc ttccaagaat ttcaaggaat cgtaacgaaa
    36721 acgttgtcaa cagtgattat acctttatta cctttataca tagcaggaac ttttgcaaat
    36781 ataactatag caggggaagt atggacaata ttaggggtat tctggaaagt atacttagta
    36841 gtaatacctt tacattttgt atatatggca tgtcaattca cagcagcagg tgtattttca
    36901 ggaaaaaatc cagtaagaat gttaaaaaat caagtacctg gatatttaac agctatagga
    36961 actcaatctt cagcagcaac tataccagtt aatgttggtt gtgcagaaaa aaatggagta
    37021 agtaagcaaa taagagaatt tgttgtacct ctttgtgcaa caatacattt atcaggtagt
    37081 atgatatcaa taacaggatt tacaactgca gttttaatga tgaatggtat gccacatgga
    37141 ataggtgtat tattccctta tattgcaatg ttaggtatag caatggttgc agcaccagga
    37201 gcaccaggtg gggctataat gagtgcactt cctttcctac caatggttgg aataccatca
    37261 gatggtgggc ttgcaagttt aatgatagct ttatacttaa ctcaagacag ttttggtaca
    37321 gcatgtaatg tatcaggaga taatgcaata gcagtagtag tagataaaat aaatgatgga
    37381 atgagtaaaa aaggaaataa agaagagcaa agtgcttagt tttttaataa tcaaaaatga
    37441 gttatctcaa atagagatag ctcatttttt atggtataaa aaattataaa ataaagtgat
    37501 ttttttattt aaaaattgct ttaagagatg atattaataa tttattttca ttaggtttta
    37561 atatacatac tctaaagaaa tactcatcta gaccttcaaa tgaacaacaa tctcttatta
    37621 ttattttttg tggaagcaat tgttctcgaa gagatttagc agtcaattcc tttgtcttga
    37681 ttttacacaa tatgaaattg cctttagtat tgtatatatc taagcttttt atattcttaa
    37741 gctctttaag caggtaatct ctttcagtgt tcattaagga aatagtattt gatataaaat
    37801 ctaaatcact aaacatgatt tcacccattt tagaagctat tatgtttata ttccataaat
    37861 ctagattttt atttatttca tttttgacat tagtgttaga tataagacca tatccaagcc
    37921 ttattccagg cgttgaaaaa aattttgatg tacctcttat aacgaataaa ttagaataat
    37981 catcaactaa ttgagtacaa gagtatgtat ctgtatcagt aaactctaca taagtctcat
    38041 caatcattaa aaatgagtct gtatttttta aaatttctct cacttctact ttagtaaatg
    38101 caaatccagt tggattacta ggattacaga ttactaccaa atcataatct ttagcattga
    38161 tagtttttat aagattttct aaatttatat gaaaattatc ttcttctttt gcaaagtatt
    38221 tttctataga acaattaatt ttagataact ctttttcata ctctgaatag gctggagaga
    38281 gtaaaagagc ctgtttaggg ttaattgtat gtataaatga tgaaattagt tctgtagctc
    38341 cactgccaag tactatatta tctatagaac atttgcagta attggatata gaagttttta
    38401 agtctatgta atcagggtca ggatacatag ataccatatc tatattgttt acaatgtatt
    38461 gtttggctaa acttgatgta ccaaaaggat ttatatttga gctaaaatcc ataaattctt
    38521 cctttgaaaa gccatactta ctagataaac tatataaatt agctccatgg tttgtgctca
    38581 taaaagtacc tccaaaaata aaattatata atcgataaaa ataactgatt aatatatgta
    38641 catatagatt ataatacaaa ggaatcgtta ttccaaattt ttaatggaaa aagcaaaaaa
    38701 gtatggataa gctgttaatt taggtttaaa aatgatgaaa aagattttaa ttgatatttt
    38761 ggtatgctat aatatataag ttaatagaag gaaagcggga ggatacaaaa tgtacaaagt
    38821 tacaggaaaa atgttgacag aagaacaaat aaaagaaaaa gtttatgaat taggaaaaaa
    38881 gatagaagaa gactttaaag gcgaagattt actagtggta ggtatactta aaggagctag
    38941 tgtatttgtt tcagatttaa taagatgtat agatttggat gtaaatatag attttatgag
    39001 tgttacaagt tatggaaact caacagagtc ttctggaaca gttaaaatat taaaagattt
    39061 agatgtagat attgaaggaa agaatgtgtt gattgtagag gatataatag attctggttt
    39121 aacattaagt aatctagttg cagctttaaa aactagaaat ccaaaatctt taaaactatg
    39181 tactctactt gacaaacctc agagaagaaa agctaatata cctgtagact atgttggatt
    39241 tgttatagaa gacaaattta ttgttggtta tggaatagac tatgctgaaa agtatagaaa
    39301 tttaccttat ataggtatag ttgaggatgt agaatagatt taaatgtgaa tcaaaagcac
    39361 taatttctaa gggagttagt gctttttagt taattatata aaattttagt aaaggagaca
    39421 tctatatgga tattcagtta aatatattat tatttttcca aaatttaaga aatccaattt
    39481 tgaactttat atttcttgtc tttacaataa gcacagaagc accgttatta atattaatta
    39541 caacaattat atactggtgt ataaataaaa aatgtggaca gaaaatactg tttgctataa
    39601 ttggaaattt tgttgtaaat ttaggcatca aagagtttgt aaaagctccc agaccaatag
    39661 gaataaaagg tcttgaatca ttgagagttt ctactgctgg tggttattct tttccaagtg
    39721 ctcatactca aactgcaaca acattttggg ttagtattat gactatattt aaaaagagat
    39781 atcttcattt aataggtgca gttatggttt taggagtagg attgtctagg ctgtatttag
    39841 ctgttcattg gccaatagat gtgatagcgg gttggatatt aggaatattt tttacagttg
    39901 tatttgtaaa aatatttgac tatattgatg ataataaaaa ttatatttta cttgtagttt
    39961 tacttatacc atttataata gtggctatat ttctaaatag ccctgaatat tttgaaaaat
    40021 ttggtataat agtaggattt gtatttggat atatggttga ggatagattt gttaaattta
    40081 atacagataa taataacaaa aaaataaatt ttagtaataa aaataagggg agtaaaaata
    40141 taattttttc aagaatatgt aggtttttgg ttgggatagt aacaatagga atgttatata
    40201 ttggcattaa gttgtttatt tcaatgttaa tagtcacttt aaatataagt gactctacta
    40261 tgagtataat ttttatgaat tttattaaga atacagtagt tgttttttat ggaatagcag
    40321 gagctccagc attatttaaa cttttaaaat taaattgatt ttaaggtata gagtcaatag
    40381 tttaaattaa aaaaattaag ggtagaaaag agtgattatt tgaagaaggt agtaataata
    40441 ggtggtggac cagcagggat gatagccgca tcaactgcat gtgagaaagg ctatgatgta
    40501 acattaattg agaaaaatca taaattagga aaaaaacttg caataacagg aaaaggtaga
    40561 tgtaatatta ctaacgcttg tgaaatagag gaattaatag aaaatgtacc tacaaatgga
    40621 aaatttcttt atagtgcttt ttatactttt acaaatgatg atgtaataag tatgtttaat
    40681 aatcttggag taaagactaa aacagaaaga ggaaaaaggg tttttccaga gagtgataaa
    40741 gcatttgata ttgtaaatgc tcttgaaaga caacttaaaa gtaagaaagt aaatatatta
    40801 ttaaattcga aagtagaaaa aataatatct aaaaataata aaattgaaaa agtaatttta
    40861 aacgataaaa aagaaattaa gtgtgattca gttgtagtag caactggtgg attaagttat
    40921 ccacttactg gttctactgg tgatggatat aaatttgcaa tttcacaggg gcacacaatt
    40981 atagatacaa aaccatcttt aattggtata gaagtacaag aaagttttac aaaagattta
    41041 gaaaaattat cattgagaaa tgttgaaata agggtgttta atagcaaaca aaagaaagtt
    41101 tatagtgatt ttggagaact tgaatttact aaatttggtt tagatggacc tataatcaag
    41161 tctgcaagtt gtagaatgaa agatacaagg aaagaaaatt acactatatt attagattta
    41221 aaacctgcat tagatgagga aaaattggat aaaagagttc aaaaagattt tcagaagtat
    41281 acaaataaaa aatttgaaaa agcattagat gatttacttc caaaaaaact aatacctata
    41341 ataataaact taagtgaaat taatccaaat acggttgttc accaaatttc tagagagcag
    41401 agaaaaaact tagtccattt attaaaaaat ttaaagttta cagtaaagag atacagacct
    41461 attgaagaag ctataataac ttctggtggt gtaaaagtaa acgaaataaa ttcaagtaca
    41521 atggaatcaa aattagtaga aggtttattt tttgctggag aagtaataga tatagatgca
    41581 tatacaggtg gattcaattt gcaaatagct ttttctactg gatatttggc aggttttaat
    41641 tgctaaaaat tacctgaatt aaaaatgttt aaaatcacat aatattatca gcaacacaaa
    41701 ataaattaaa cagaaggaag gtaatcaaca tggcaagtaa caataacaac aacagaacag
    41761 tagtaccagg agcaaaagag gctttaaacc aaatgaaatt agaaatagct aatgaacttg
    41821 gtatgagtaa ttatcaacaa gttgataaag gaaaccttac tgcaagagaa aatggttatg
    41881 taggtggata catgactaaa aaattagttg aaatggctga acaacaaatg gctggaaaaa
    41941 gcaacagata gttataaata attatttata actattggct attagtttag ctagagctat
    42001 ctcaaatata gaaagtaatt tctaaattga gatagctttt ttttattaaa ttattaaata
    42061 gatatatttt tatgtaataa attttatgta tttggataaa ataatacaaa tgaatatgtt
    42121 acataaggag tgatgtaaaa tgagtagtgt aaattttgag gatttaaaaa acaaatttat
    42181 taatagtgac ttagatgaaa aaataagaat atatacaaca acagaaggtt tatcagtaga
    42241 acaatttaaa gagctactaa aatattatcc aatacaacat ttatctaaac tagaaaaagc
    42301 acttggatag cagtatttaa tccaaaatta aaaaggcaat ttatttaata ttaaaaatat
    42361 tttttattaa ataaattgcc tttttataat aggtttaaat tattatgctt ttgatatata
    42421 attatagatt ctctcatttc caagttcaat agacccagta ttaataagag taactattcc
    42481 ttctatatct ctagcagtta agtatttata tggaattaat aaattataac cagtattact
    42541 tagtaagttt tttaattggg ttatttgaga gatattgaaa cattttgcag ttttagtaga
    42601 tttttttctt aagttagata aattttcatt ttgaatattt attaaagttt ctatatagtt
    42661 tctataagat atcttaaagt ttgtctcatt gactattttt tcattaagta tactactgat
    42721 aaaatcttga gacaatatat tatctgcaaa tttatctgct gttaattttc cagatgttat
    42781 gcctttttga tattttctaa gaattttttt agtatagcag tatattttat ttctcgtttc
    42841 taaatcgagt tgttctattt gcacaaaacc acttccttct aattataaaa atatttatta
    42901 aaaatctata cttaactaat aaagcttcaa taacttattt acaataaata ctaaatcata
    42961 aaatatttgt tattttttat tattaccaag tataaaaaat attataattg taaaacttac
    43021 aaatatatgt aaaatattta ggagtgatga aaatgttaaa tgggataata aagctttttt
    43081 attctgaaat tgtaaaaaca aagattaata acttagattc aagtggatat tctttaagag
    43141 atgaatgtaa agaaatcaga aatataactc taaagataga tagtgtattt tttaagcttt
    43201 taaagttagc ttttggttca atattgctta tggtagcttt taatttaaac atggttttag
    43261 gtataggagt gtttttagtt ggtgtagttt atatactata taaaagaaat atggaagaac
    43321 aagtaaaaga gcatataaat aatgtgaaaa ataatatcga catctctaaa ataactttta
    43381 tggatgaaaa agggagaaca ggtctaaatg cattaattac attacttctt ataggattat
    43441 tgacaggatt taactggata ttagttgcta gttttgtcgt tgtttttgtt tttactataa
    43501 agaatgtatg ttaaaatact aataaatagt ttaatatata gggggagaaa ttgagtatga
    43561 ctaatggcag ttataaaatg aagaatttag tggaaatatc cgctatggtg actgaatcta
    43621 cagatttttt taatataaaa gatgacataa tagaaaaaat gttagaagtt gttcacccta
    43681 caaaggcatg tgtgaatcta ttttataaaa atgattctaa atatgcctac ttagtatgtt
    43741 cgcaaacctt agaatatgta cctcaaattt ttccaataaa tagtcttaaa ggtgcaaaaa
    43801 ttgattttga cacatatcca gagtatatac atgaggctgt aaatgaaaaa aaaataatat
    43861 atgtggaaaa tgtatttgaa gattctagag cagaaggtga acgagattta gcaaaagctg
    43921 aaggttatat tgggagaata gtttttcctt ttataataaa taacgtagtt gttggattta
    43981 tgacttgttt tttaaaagag gatgattata taacagaaca agatatagat tttatatcat
    44041 ctgttgcatc actcttaagt ttatcaatag aaataactaa taaaaataat aatgtacaaa
    44101 ttttaattga taaactaaga ggctcaataa gtgccataaa tgaagctact aaaaaactat
    44161 accttaataa gagtattgat ggatttttgg aaaatttaag taaacaagcg tgtaatatta
    44221 ctaatagtaa agaagcttta ataattataa atagtcatca taataaggat gctatagtta
    44281 gttgttataa tgttgagcaa aaaaagaaaa caaatttata tcctatgatt gatatgtttt
    44341 taaagaaaga gtcattgggg gggtatttaa ataatttaaa accatgctca aaaaagggaa
    44401 taaatttgga aagttatata tattacaaac ttcaggataa aaatgagact ataggatgta
    44461 tagtttgtgc aaatagcaaa aactatacta gtgatgattt aaatattctt agtatattgt
    44521 ccaaacaagt ttctgttggt atgcaattat atgaacataa tgaaaaggaa gtaaaacaca
    44581 aagttcttga aaacgagttg aacatattaa ataaacaaca acagcttatt atggataaag
    44641 gtaaaatgga ttgtaatgat gagaaagaac tttattttta ccataaacca gcaagggtag
    44701 taggtggaga tttttattat gctacaaaag tggatgatga acatatagtt tatattgttg
    44761 ctgatgtaat gggacatgga atggtatcaa attatgttgt tgcaatgata aaaggtgctt
    44821 ttaaagtatt atgcaatcaa tacaatacac caagagaaat aatgacaaat ttaaatagga
    44881 tgttatatga tgaatttgac aagatgggag tatttacaac ttgtttgata ggtattatag
    44941 atactaaaga aaatatgatt actgtgtcta atgcaggtca ctatagtcct gtagttgtaa
    45001 agaaagatgg cacaatagaa aataatttaa attgtaaaaa gggcatacct attggaataa
    45061 tggaagatgc tacttatgaa aataatactc tttcaatgga agattatgca atggtatgta
    45121 tgtacactga tggaatttta gagataaaaa attcatcaaa agaagagtat ggtataaaca
    45181 gattagaaaa ctttctaaaa gaaaatttta gattaaatca acagttaata gtagaaaatc
    45241 taaaattgga tttaaagaac ttctcatcaa aagataatta tgatgatgat attttaatag
    45301 ttatgttaaa ggataggtag attgattatg gattcaaacg ataaaaaagg acttaaaagt
    45361 ccagagatta taataataaa tggaagtcca agaattaata aaaattgcag ttttatagca
    45421 aatgaagtaa aagctatgct agaagagtgt ggattaagtt gtaaaatatt taatatctac
    45481 aatatggata ttgaatattg cacagcatgt ggattttgtg aaaaaacagg gtattgcaag
    45541 tttaatgatg atatgaagcc attatataaa atgtttgatg attcaaaagg aactatagta
    45601 ataagtcctg tacattttga ttcaatatca gctaaattaa agactgttgt tgatagaact
    45661 caagctatat atgcaagtaa atatgtatta aataaacctt caattgatag aagtaagaaa
    45721 cggataggaa tgtatattgc agtgggaggg agtaaatcat atgattcaca gtttaaaggt
    45781 ggacagattg ttatggattt tttctttaag agtataaata ctaaactttt acacaatata
    45841 tatttaaaca atacagatga tgtatcttat atagataatc aagaatttaa agatataata
    45901 tataaaaatt taaagatata tattgattca atataatttg attttgtagg taatattatg
    45961 tataattgta ttgcaataat ttaaataatt aaaaggaggg aatttcaatg aaattaccaa
    46021 tagcattatc taataaacat gttcatttga gtcaagctga tatagacgta ttatttggag
    46081 caggtcatga gttaactaaa tttaaaccat tatctcaacc agggcaatat gcctgtgaag
    46141 aaaaagtgga tttagtagga ccaaaaggaa ctttaaaagg agttagagta ctaggaccag
    46201 ctagagctca tactcaagtt gaaatatctt tagcagatgg tttcccatta ggagtaaaag
    46261 caccaataaa agaatctggt gatatagctg gaactccagg tgttaaatta gtaggacctg
    46321 ctggagaagt tgaaatgaaa gaaggagtta tagttgcttc tagacatata catatgagca
    46381 atgaagatgc tgctaaattt ggtgtaactg ataagcaaat agtaaaagtt aaaacatctg
    46441 gaccaagagc attagtattt gaaaatgtat tagtaagagc tagtgacagc tttaatctag
    46501 aaatgcatgt tgatatggaa gagggaaatg cagcaggcgt taaaaatgga gatttagtag
    46561 aattaatagt tgattaatat tataaataga ctttatgcta gtagatattt tatttgctag
    46621 catattttta aatgtaataa atttctatag gataaattta atcaattaga ttaatttata
    46681 aaataaaggg gtattataaa aaatgtaata aaaacttaca aaaatatgat gtaatatggt
    46741 aaaattatat atataaaaat ttacatgtaa taaggataag cattttactt tttatactaa
    46801 tatgaacaaa tagataaaaa agtttgaaaa atctaactta atttattata tattgtatac
    46861 aaaattatga aaaaaactta acatatttaa ttttaaaaaa aatctactta aatatgtaat
    46921 taatttacaa ttttcaaagg aattttatct aatctgtagt attatattag aagatagagt
    46981 atttgcgcga ctatatgtgt gtttatagtt ttgttgtgta aattatatgt aaatataaaa
    47041 aaatagactt gttataaaca agtacaattg tagctatcaa ggaggataca tattatggct
    47101 aagtttatga aaacacttga tggaaataca gctgcagctc atgttgccta tgcgtttaca
    47161 gatgtagcag ctatatatcc aatcacacca tcttcaacta tggctgaagt agttgatgag
    47221 tgggcatcac aaggaagaaa aaatatattt ggacaaacag ttaatgttgt tgaaatgcag
    47281 tcagaagcag gtgcatcagg aacattccac ggttctttac aaggaggagc tttaacttct
    47341 acttttacag catctcaagg tttattatta atgataccta acatgtataa agttgcagga
    47401 gagttgttac caggagtatt ccatgtaagt gctcgtgctt tagcatctca agctttatca
    47461 atatttgggg atcaccaaga cgttatggct gctagacaaa ctggatgtgt attattagct
    47521 tctggttcag ttcaagaagt agctgatata gcacctgttg cacatttagc tgcaatagaa
    47581 ggtagattac catttataca tttctttgat ggattcagaa cttcacatga aatccaaaaa
    47641 gttgaattac tagaaaatga agattatgca agtttattaa actttgaagc agttcaagca
    47701 tttagagata atgctttatc tccaaatcat ccagttgctc gtggtactgc tcaaaaccca
    47761 gatatatatt tccaaactag agaagcttct aataaatact atcaaaatat agtaggtata
    47821 gttgaaaaat acatggaaaa aatgagtaat ttaacaggta gaaaacatag tttatttgat
    47881 tactatggag cagaagatgc taaatacgta ttaatagcta tgggttcagt aacagaaaca
    47941 atagaagaaa ctatagatta cttaaatgct aagggagaaa aatatggttt agttaaagtt
    48001 catttatata gaccattctc aatgaaacat ttcttagatg ctatgccatc tactgttgaa
    48061 agaatatgtg tacttgatag aactaaagag ccaggttcaa ctggtgaacc attatactta
    48121 gatgtacgtg atgtattcta tggaaaagaa aatgctccta tgataatagg tggaagatac
    48181 ggattaggtt caaaagatac tactccttca gatataaaaa ctgtttttga taacttagtt
    48241 tcagaacaac ctaaaaatgg atttacagta ggtatagttg atgatgtaac taatacttct
    48301 ttaactccat ctgagccaat aaaaatagct tctaaaggaa ctataagatg taagttctgg
    48361 ggattaggtt ctgacggtac tgttggagct aataaacaag ctatcaaaat aatcggtgac
    48421 catactgaaa aatatgctca agcttacttt gattatgact ctaaaaaatc tggtggtata
    48481 actatgtctc acttaagatt tggtgatact ccaataagag ctacttattt aatagatgaa
    48541 gctgattata ttgcctgtca taaacaatca tatgtttatc aatatgattt attaaaaggt
    48601 cttaaaaaag gtggtacatt tgtacttaac actatatggg accaagcagg acttgaagaa
    48661 catttaccag ctcatatgaa acaatatata gctaaaaatg atataaaatt ctatacagtt
    48721 aacgctgtta aactaggaca agaaataggt cttggaaaca gaataaacat gataatgcaa
    48781 tctgcattct ttaaattagc tgaaataata ccagaagaag atgctgttaa atacttaaaa
    48841 gattctatag ttaaagctta tggtaaaaaa ggtgaaaaaa tagttaacat gaactatgct
    48901 gctgttgatg caggaataaa tgctttagtt aaagttgaag ttccagcttc ttgggaaaat
    48961 gctgttgatg cagataaagg agaagttaaa gaaccagaat ttataaaaaa tatactaaga
    49021 ccaatgactg cacaagaagg aaacaactta ccagtaagta cattcaatgg tatagaagat
    49081 ggaacattcc cttgtggtac tgcagcttat gaaaaacgtg gtatagctgt tgatgttcct
    49141 gaatggataa tagataactg tattcaatgt aaccaatgtt cttttatatg tcctcattca
    49201 tgtataagac cagtattagt tactgaagaa gaattagcta atgctccaga aggatttgaa
    49261 gctaaaaaag cattaggtaa aggattagaa ggattaaaat atagaatgca acttagtcca
    49321 cttgactgta caggatgtgg aaactgtgct gacatatgtc cagctaaaga aaaagcttta
    49381 gttatgaagc caatagatac tcaacttgat acagagttag ataattgggc atttgcagta
    49441 aatcctgatg aggtagctcc aaaaggtgat gttatgcctg caaacactgt taaaggaagt
    49501 caatttagac aaccattaat ggagttttca ggagcatgtg ctggatgtgg agaaactgca
    49561 tacattaaag tagttacaca attatatgga gatagaatga tgatagctaa tgctactgga
    49621 tgttcttcta tatggggagc ttctgcacca tcaattcctt atacttgtaa ccatgaaggt
    49681 aaaggtccat cttgggcaaa ctctttattc gaggataatg ctgaatatgg atttggtatg
    49741 tacacagctg ttaagcaaat aagaaataaa atagtagatg ctatgacaga gttagtttct
    49801 atggatatct gtgaagatgc taaagcggta tttacagagt ggttagattc tagaaatgat
    49861 ggagaagctt ctaaagtagc aagtgctaag gttgttgaat tattagaaaa accagcttgt
    49921 gattgtacag atgaaaaagc taaagagtta gtaaaagcta taaaagatag aaaagattat
    49981 ctagttaaga gatctcaatg gatactaggt ggagacggtt gggcatatga tatcggttac
    50041 ggtggattag accatgttct tgcatctggt gaagatgtaa acgtacttgt atttgataca
    50101 gaagtatatt caaatacagg aggtcaagct tctaaagcta ctccagctgc tgctatggct
    50161 aagtttgctg cttcaggtaa aaaatctaga aagaaagatt taggaatgat ggctatgaca
    50221 tatggaaacg tatatgtagc tcaagttgct atgggtgctg ataaaaacca atttatcaaa
    50281 gccgttatag aagctgaaaa acatgatggg ccatctttaa taatagctta tgctccatgt
    50341 ataaaccatg gattaaaaga aggtatggga agaactgtag ctaacgaagc acaagctgtt
    50401 gcttgcggat actggcattt atacagattc aatccagaag taaaagctga aggtaaaaac
    50461 ccattcactt tagattcaaa agaaccaact gcaagcttca aagatttcat acttgcacaa
    50521 gtaagatatt ctgctatagc taaacaattc ccagaggaag ctgatggatt atttgctaaa
    50581 gctgaagctg atgctaaaga aagatatgaa ggatacaaaa aattagctga ataatattaa
    50641 agtattataa tgcttatata gaattgataa aagagagtgt ctcaaagtga gctttttagt
    50701 tcatgaggca ctctttttgt atgaaatcaa atatatttta atcatttcaa caatgaaaaa
    50761 ggccttatct ctattttgag acagcctttt tttatgaaga tattaagatc ctgtttttac
    50821 ctattaaata tctttttaag ttttccattt tcatcttgcc aatacttttt atatattgct
    50881 ctacacacta aaattaagag aacaaataat atttatagta tgtatatttg gatttgataa
    50941 ttttaaaata gatacaaaag agtatctcat ttgtgttttt tatcacttag agataatctt
    51001 ttgatatgta tggaaaaaga ttattttatc ctacaaagat ttgtgtccaa taaattgaac
    51061 caccactttt ttgtgctact ccaacaccta ttttactgaa atttggattc attatatttt
    51121 ttctgtgacc ttcagaattc atccatgcat ttacaacttc tttaggagtt ttttgaccca
    51181 tagctatatt ttcacctgct gtcttatagc ttattccaaa ttttttcaac atgtcaaatg
    51241 gagaaccata agttggagaa ttgtgtgaaa aatagttgtt atcaatcata tcttgagatt
    51301 tttttgtagc aacatttgat atagaagaat caagagtaag aggatttaat ccagctttag
    51361 ctctttctat atttactaag tcaacaactt ctttttggta agctgaaaaa ctttcacttg
    51421 tagagcctga gttgttatta tcatcaggtt tgttagtaga gtctgaatta ttgttgtctt
    51481 caggtttatt agactcaggt ttattattat caggtttatt agtagagcct gaattattgt
    51541 tgtcttcagg tttattagac tcaggtttat tgttaccagg cttattagta gagtctgaat
    51601 tattgttgtc ctcaggttta ttagtgttgt tgcaattctt gtcattatga ttcttatcct
    51661 tacaagagtt atctttatca ggcttaagtt ctggttttac agaacaattt ggttttgcag
    51721 aaaacttgta tttagtatta ctatatacta aacttttgtt acttatattt acattatttt
    51781 caagtgatga agcacttgca gtaacagcac ttgaagctgt tgctaaagtt aacactccta
    51841 aagctaatat agtttttaat tttgttggta tatttcttct catcattaaa cacctcttaa
    51901 tctaaaaatt ctaaaaatgt tactgatttg taacctttag tgtagggggt tacccgtgaa
    51961 tctatactaa cataagatta agactttttt tactgtaaag atatgtaaaa aagatatata
    52021 tggaataaat aggtatattt cagtgtgttt gacaatttgt attaaaagcc tgtatagtat
    52081 ggaattactt agttagagaa aataatattt ttataataat tccataacgt aaatttatag
    52141 ttatgataaa ttttttttta tttggttaaa tttattcagt agatgctttc gtattagcaa
    52201 caaattataa ttatttaaat ctttaagaaa taaaaagggt ctgtctcaaa atagagataa
    52261 ggcctttttt attgtttaaa tgataggcat atgttatttc atataaaaag agtgtctcat
    52321 taagtaaaaa gttcactttg agacaccacc tttcatatta tgaattttaa tttgttttat
    52381 attaatacat tagtaatcct aatgtaatta caataatttc tgctattagt gaaccaactg
    52441 caactacaat gaaagtttct ttaaatccag ttttatgtgt taacttagat aagcttaaga
    52501 atgtaagaaa aacaccactt tgtggaacta tagttagtat atttgaagct attgtagcaa
    52561 cacggtgaat taattctgga tttagaccag tacttaaata ataatctgca aagtttggca
    52621 ttactattcc taatgcacca gaagaggacc ctgttattgc tgacattgtt gaagtcaaaa
    52681 cagtaagact tataagtgga ctacctggta ttgacattat aagtttgcta aatacatcaa
    52741 aaccaggtgc agccattatt actgctccaa atgcaactgc agaagatgta gcaaatatag
    52801 gacctataga accactagca cctatgctca atgtctgtat cttagatgat agaaatttat
    52861 ggaataaaaa agcagataaa agaattgcaa ctattaaagc tatgtaaatt acattttttt
    52921 tcataaattc tgaaccaaaa gtacttccag ttatagctat aattacgagt gctaatagag
    52981 gaaaaatact tgcaaaaaat cctggtaatt ctctatctaa agattcttct tctaattctg
    53041 aagtataagt tgcataagtt tcaccttttc taatactttt gtttagagca tatttcatat
    53101 agacaagtcc aaacgtagca caacctatac tacctacaat acttggtata gtagctgcag
    53161 taagagacgt atttagatat tgaataggta taacattttg aatggctggt gttccaggaa
    53221 ttatagtcat agtaattgtt gcaataccaa gccaaagtgg tatctgaata aggttccaag
    53281 aaatatcaag ttttttaaat agagttcttg caagtggaat gaccgcaaac ataactacaa
    53341 ataagcttat accaccatac gttaatataa aacttactat aaatatagca actaaaactc
    53401 tataaggatg attagagcca aatttattta aaataaaatc agctattgat acagtagctc
    53461 cactttcttc cataaattta gcaagtattg agcctaataa gaatattgca aaataactca
    53521 ttatatagtt tccaagtgca cccatataat tgtttttgtc agcacctagc attgatgtta
    53581 caacatccat tttatttaaa ataacaacta ttaaagttgc tattggagca gctatagtta
    53641 tatgaatttc tttaactgaa aaatatataa tagctatgat accaataatt ataccaattg
    53701 gtgcgataat actcataaat atccctcctt agtagaagga cctgaagaat actttcttca
    53761 agccctaaat ataataacta ttgtgcagta tatcctccat ctagtaagca agcttgtcct
    53821 gtaataccct tagcattatc acttgctaag aacatagcat agtcggcaat ttcttgaaca
    53881 tctaataggc gtttttgtgg tacaagtggg tataaaactt cctctaagac attttccaat
    53941 ggtatattac gagtttttga aaggtcattg aactgacctc tgacaagtgg agtatctaca
    54001 tatccagggc ataaagcatt aactgttata ccagaatcgg cagcttcaag agcagcaact
    54061 tttgttagtc caataagtcc atgttttgct gagttataag cagcctttcc agcaaatcct
    54121 attacaccat tgattgaggc catatttatt atacgaccaa agccttgttt tttcattata
    54181 ggaaatgctt ttttagtagc tataaaaggt gctgtcagca ttatttttat cataaattca
    54241 aatttatcag ttgggaaatc ttctatcata gaaacatgtt gaagtccagc attgtttatt
    54301 aatatatcta atctgccata cttttcaaca gttttgtcta tagcgtggtt aatttcttct
    54361 tccttagtta catcacactt tacactcatg caatcataac cttttttttg taactctgtt
    54421 atggtattat ttaaagcttc ttgatttata tcagagaaca ttactttagc accatttttt
    54481 aagaacgatt cagctatttg tttacctatt ccacttgcag ctcctgttac gaatacaact
    54541 ttatctttaa ccattttcat ttcctccaat tttattttat ttttatatta atattttaag
    54601 caatacaaat ttatatgtaa tacgtctaag agatttttat tttctagttt taattatttt
    54661 aaacaacaga tatttttcca tttaatgaat ctgacaatat taaaggagcc tcagtagcag
    54721 aaataacatc atctaatgtg aaagctggat ttatttcttt aagtaaaatt ccatctgatg
    54781 ttacttccat aactcccatt tcagtaacta tcaaatctac agcatttgtt gcagtaagtg
    54841 gaagagtaca attatttaat atttttgcag aaccttttga agtatgttcc attgctacaa
    54901 taactttttt agctccagtt actaggtcca tagctccacc catacctgga accatcttac
    54961 ctggaatcat atagtttgct atattaccat atttgtcaac ctgaagtgcg ccaagaacag
    55021 ttatatcaac atgacctcct ctgattattc caaaagaagt agcactatca aagaaacatc
    55081 ctccaggaag tatagtaact ggttgaccac ctgcatttac aatagtttca tcacttgaat
    55141 ttccatcttc ggctgaacct aaacctaaaa agccattttc agattgtaag ataacgttta
    55201 cattatccgg aatataattt gctactttgg ttggtagacc tattccaagg tttacaacgg
    55261 ctccgtcttt taattcttta gatactctat ttgcaatata ttcttgcatt tctaatttat
    55321 ccattataaa ttacctcctt caatgatgta attcacaaaa atacctggtg tattaacttc
    55381 atttgggtca atatcaccaa tttcaacgat ttcatctgct tcaactattg taattttggc
    55441 agcagatgcc attaaattat taaaattgtt catagagcct ttataaacta gattacctgc
    55501 tttatcgacc tttgatgcaa atagaagagc aacatctgca taaattggtt tttcaagaag
    55561 gtattctttg ctatcgacaa caattttttg ctttccctcc tcaacaagag taccaatacc
    55621 agtcggtgtg agtatacctc ctagaccata accagctgca cgtatttgtt cagcaagagt
    55681 tccctgagga actaactcaa catctgtttc attgttattc atttgtcttc cagtttcttt
    55741 atttaatcct acatgagaag caattatctt tttaaattgc ttatttacaa ccattttgcc
    55801 aactcctttg tcaataaagc cagtatcatt gcaaattagt gtcaaatctt ttatattttt
    55861 actacataaa taatcaatta aattttctgg tgaaccattg gccatgaatc caccaaccat
    55921 tattgtatct ccatctttta catgagatag agcttcatca atgctaacta ttttattcat
    55981 atttcctcct atcgtgaaac caccattgat attccttgac caccaccaat acaaagtgtt
    56041 gcaagtccag tttcaacttt acgtttttgc atttcataaa ttaaggtaac aagtattcta
    56101 gctccacttg ctcctattgg atgtcctaat gctattgcac caccattaac atttaacttt
    56161 gatgaatcta tttgaagttc atttttaaca gctaatgctt gggcagcaaa cgcttcattt
    56221 gcttctatta agtctatatc attgatgcta agtccagctt ttttcagtgc ttttctagtt
    56281 gcagggattg gaccagtacc catgacttca ggttcaacac cagctgaagc ataggatttt
    56341 attttagcaa gtggtctaat acctagttcg tctgcttttt gttggctcat tagtattaac
    56401 atagccgcac catcattaat acctgaggca tttccagctg ttacagtacc atcttttttg
    56461 aatgcaggtt taagtttagc taaaccttca aatgtcatac ctaatttagg atattcatct
    56521 ttatcaatag tttctatttt tcctcgacgt attaaaacat ctactggaac gatttcttct
    56581 ttaaagcggt tgttttttat agcattctca gccttacttt gactttctaa tgccaattta
    56641 tcttgcattt ctctagtaaa ttcaaattta gttgcaatat tttctgcagt tattcccata
    56701 tgatactgat tgaaagcatc aataaggcca tctgttagca tactatctac catggttatg
    56761 tcacccattt tactaccaaa gcgagcagtt ggaacgatat aaggagcttg gctcatattt
    56821 tcagtaccac ctgcaattac cacatcattt tcaccagatg tgattgattg ggcagctagc
    56881 tggacacttt taagccctga accacataat ttatttactg tgtagcttgg aacagaattt
    56941 ggtatacctg catttatagc aatttgtcta gcaacattct gtccaagccc tgtttgaaga
    57001 acatttccaa ttattacttc atctatttca gataaattaa gtcctacacg tgatatcgct
    57061 tctttaacag tgatagttcc aagttgaaca gctgaagtat ttttaaatac acctccaaaa
    57121 cttcctatag gtgttctgac tgctgatacg attactacat ctttcatatc taatagttac
    57181 ctttcttgaa tgataattaa aaattttctg aataaagaaa ttaggaaaca ctataataaa
    57241 gtactttttt aatacaagct tgtttttaag tatttcaatt acttttattc ctaatgcttt
    57301 aggttttcct gtttctaatt atagattacg gcgaaaaaag cgttttgtct aagtctaatt
    57361 ttttatatag tgtataagaa aaatttatag gataaaaatt tatagtagat ttagaaaaat
    57421 attttgttaa aatattaatg aaattatgtt gattttccta taaaatatca ttagaagaaa
    57481 aaatataatc taaattatac ttttggggaa gggggtttaa aatggatata aaacagttga
    57541 aatattttgt tgaaatagtg aaatctggtt ttaatttatc aatagcatct aagacacttc
    57601 acatatctca accagcatta agtcaaatta taaaaaattt tgaagaaagt gaagatgtat
    57661 atttatttga aaggtataaa ggcaggttaa atgggttaac accagcagga gaaagatttt
    57721 atgtaaatgc agaacatatc ataaatgaat acaaaaatat gatggaagat ttaagagaag
    57781 attcagttca atttaaagga aaagttagaa taggaatacc tccacttata cttggaatag
    57841 cattttcaga tgtagttgca cagttagttg caaataatcc agatatagaa tttgatatag
    57901 tagagaaggg tgcatatgac ttaaatagaa tgcttatctt acaggaatta gattatgcag
    57961 tattgttaca tccacataaa attgataaaa gtgtagtaac tgaacatgtg cttcaagaag
    58021 atgaacttac agcattttta aatataaatc atccacttgc taagaaggat aagatagatt
    58081 ggaacgattt gaatgagcag ttacttgcaa tatttaatcc tacttttatg atacatcata
    58141 agctaatgca aaaatttact gatgaaaatg ttaagttaaa aagatatata atgtctggtt
    58201 catgggattt tttgttgcta tcaaccacaa attctgattt tataacaatt ctaccatcac
    58261 cagttcatga tttttttgaa aatagcaaaa ttatagaaaa gccttttaat catcctatta
    58321 catggaaagt tatattatgt agacctaaaa aagaacgtta tagccatgta gaccaacatg
    58381 tatttaaatt tattatagat ttctttagta aaaagaactc ttaaaaatgt tattaaaata
    58441 acaaataaat aaatcaattt ttattataat aaactattag tttttcatct atatgtttga
    58501 gagcaataat aggttttaca taacatggta gagtagtatt taaagttata atatacaaaa
    58561 tatgtatttt tttaaacgaa aaataaaaac agtgtatatc tttagataat cactgttttt
    58621 attttctata taaaatttgt taagtattac tagaaaattt tttgcttatt atagtcaaac
    58681 tcatgactgt taaaaatata agtaatcctg gaaatacagc taaatataac ttgtcaaaaa
    58741 tatgtctttg agcatcttga agcatattac cccatgatgc aagaggcaat tggattccaa
    58801 gacctaaaaa actaagagca gattcagtta agatagcatt agcaatattt aacgaaccag
    58861 caactacaat agtctctttt aagtttaaaa ctatatgtct taatagtata tttctagtct
    58921 tgataccagt agctttagca gctagacagt attcattttc ctttattttt aatgtttcgc
    58981 cacgtacaat tcttgaaact tccatccaac ttaagaatcc aataattagt ataatagtgg
    59041 aaactttagg tgttagataa gtatttaata caatcaaaac taagaaactt ggtatactca
    59101 taagcatatc tattaatctc atcaagaatt ggtctagctt acctccaata tatccactaa
    59161 taacacctac agcagtacca aatactacag aaataagcat tgataaaata ccaacactaa
    59221 gtgaaatcct tccaccataa agagctctag agaagtaatc tctaccaagt tcgtctgtac
    59281 caaagatatg ctttaagcta ggttcttgaa gtttagaagt agtatcaatt gcattagagt
    59341 ctatatttaa tataaaagaa gctataatta atatgattat tatgctaaga gtaatacatg
    59401 caaaagcaaa ggatttatct ttttttaatt tttttaagat acttgtaaac acaactagaa
    59461 tcctcctttg ctagatttta ttttagggtc aattaataag tatagtatat cagcaactaa
    59521 attacctaaa attaaaaata gtgctgataa catagttatt gccataatta cagtataatc
    59581 tcttgaaaaa attgaatcaa tcattaatct tcccatacct ggccaagaga aaactacttc
    59641 tgtaacaaat gcaccagtga ctaatttttg tatactcatg cctaggattg taaccaatga
    59701 tgtcagtgaa tttttcaaga catgtttaaa tagtatgact ttttctgata atccttttgc
    59761 tcttgctgta ataacatatt gtttttttaa ttgttcattt acactagctt ctacataatt
    59821 tgtaaaaata gcagtattat atagacctaa agttattaca ggcaaaacta aatgttttac
    59881 taagtctcca atactatcat ttccaatagt atgcatacct cctgatggga aaatgttaag
    59941 tttaactgaa aaaattgaaa tgagtatcat acctatccaa aaagaaggta tagaaacacc
    60001 tatataatta aaaacagatg atactttact agttattgaa tttttattta cagctaaaaa
    60061 tattccaaga ggtatagcta ccaatattga aaatatcaaa gaagtaccca taagaataaa
    60121 tgtatttccc attttttcca taatttgttc tgtaacgggc atatggttta tataagaaat
    60181 acctaagttt ccacttaaag tgtttttcaa ccagtttaaa tatcttatat atactggttt
    60241 atcaagcccc atattaaccc ttatattttc aatatcctgt gaagacatta aaggatttat
    60301 atatgcatct gctgggtcac caggagctaa ttctaaaagt ccaaatgata atatagatat
    60361 taagattaac ataggtatta aaacttttac tctattcaat attaatttta acataagtaa
    60421 attctcctaa ttaaaagaga tgtctcgttt taaaataata aaacaatatt atttttagac
    60481 atctcctttt ctaattaata atataaaatt ttacatcaat tattctacat acagtttaga
    60541 ccaatcttca aataatattg caggagatgg tttagcatct tttattcctt ttagattttt
    60601 ttgagctgcc attaaatttt gttcataatc tatagtgtaa ataggcgcat ccttagatat
    60661 atcctgttgt atttgcttat atatttcttc acgtttttcc ttgtttaact cagtaaatcc
    60721 tttttcccat aaagcatcta tttcagcatt agaatattta gtttggttgc aatattgacc
    60781 agtttcaaac atacctctat atgggtttgg ttctaatccc caagcataac catttacata
    60841 catatcacat tcagtgctat tactaaatag tatattgaag aatgccttac tttcataagg
    60901 aactatttca gcttctatac caattttttt caactcttgt tgagctacta aggcatagtt
    60961 tttatgccca aatctacctg tgttataacc tatttttaat ttatcaattt taacaccact
    61021 tttatccaat aagtctttag ccttatttgt atcttggtca tatccttcaa caccttcttc
    61081 tgtatagaag tctgcttctg gaactaatat agatttagca ggtacagaac cttcttttcc
    61141 atatgctgat tctatcattt catttctatt taaggcatat gataaggctt gtcttacttc
    61201 tttgttagcc atgtttgata tattttggtt aaatacaatg tagtttaatc tttcttcact
    61261 ataagtataa gtttgtaagt ttgagtttga ttttgccttt tcatatcctt cagcagatgt
    61321 cttcataagt gaaatttcgc cattatttaa agctgcttct tgagatgctt cattaggaat
    61381 tatttttagc gctatagaat ctgccttagg ttctccacca aaataatctg cattcttttc
    61441 aaatactatg ctttcacctt ttttccattc cttaaacttg aatgcaccag aaccaactgg
    61501 attattattt ttttctgact tagctatatt tgattctcct tcaaacacat gttttggtat
    61561 tggagaaatt ttagatatac catataagaa agattcactt gcagttggta aagtaatctt
    61621 aactgttaaa tcatcaattt tttctacttt aactggtttc tcacccacca ataagtattg
    61681 tcttgatggt gaattttgtt tttcgtctaa aactgtatta aatgtaaata agacatcttc
    61741 agcagttatt ggctttccat catgccattt taaattggat tttaatttta tagttaattc
    61801 cttactatct ttaaaatcta ctttttctgc taaataattt actaaatttc ctttttcata
    61861 agtataaaga ggagcataaa ctatatttga tgcaagagag tcttctttaa cgtttgcaaa
    61921 agctccattg attatatctg ggtcacttgc cataggtatt acaacagttc caccagattt
    61981 gcttccacta gacttgtctt tatcattgga agcacaggct gtaaatgtaa gcattaatga
    62041 tgaaactaag attaatgatg ccaatttttt aaatttcata ttcttataaa acctccataa
    62101 aataatattt aacacatatt ttttattgta taatagtaat cattattaaa tcaatatata
    62161 tagaaattaa ctattatatt attataatta atagaaaaaa ataataaaat gtaagaaaat
    62221 gttgtaattt aaatttttat aaaatacaat caaaaggtgt atgttatctt aagtaatttt
    62281 gctgagagga gaaaataatg agtaaagcac taattgaggt taaagattta aaagtttatt
    62341 ttcatacaga taaagggatt gtaaagagtg taaatgaagt aagttttaat ataaatgaag
    62401 gtgaaactat tggaatagtt ggagaatcag gatgtggtaa gtctgtaaca gccatgtctt
    62461 tgatgaagtt acttccaaca tcaaaaatag aaggtggaga aataatcttt cgaggcaaag
    62521 acattttaaa gatgaatgaa gatgaactta tgggaataag agggaatgag atatcaatga
    62581 tttttcaaga accaatgaca tctttaaacc cagcgtttac tgttggaagt caaatcattg
    62641 aaggtattat gatacatcaa gatttatcaa aggaagaagc taagaaaaag gtaatcgata
    62701 tgataaaact ggtagaaata ccaagagctg aagaaatata taattcatat ccacatgaat
    62761 tatcaggtgg tatgagacaa cgaataatga tagctatggc tttatcatgt aatccaaaat
    62821 tacttatagc tgatgaacca acaacagctt tagatgttac tattcaagct cagattttag
    62881 atataatgaa aaatataaaa gaaaaattaa acacatctat aatgatgatt acacatgatt
    62941 tgggagtagt tgcagaaatg tgtgacaaag tattagtgat gtatgctggc aaaattatag
    63001 aagtggcaga ggttgttgaa ttatttaaaa atccaaaaca tccttatact attggacttc
    63061 taaaatcaaa gcctgctctt ggaaaaaaca aagataaaag attgtattcg attccaggac
    63121 aagtaccaaa tccaattgga atgccagatt catgttattt tagtgataga tgtgaaaaag
    63181 tatgtgataa atgtagaact cagatacctc ctttaataga attgaatagt ggtcatagta
    63241 ttgcatgttg gttatataaa aaggaggaga tataattgaa aaaagaagag agtttattaa
    63301 aagtagaagg tcttaaaaag tactttgaag ttaaatctgg attatttgat aagaaaatta
    63361 aatctgtaaa ggcagtagat ggaatttctt ttgatttaaa aaaaggagaa actttggcta
    63421 tagttggaga atcgggatgt ggaaaatcaa ctgcaggaag aaccattctt agattaattg
    63481 aaaaaactga aggtaaagtt gaatttaatg gtgtagatat atataagtta gagaaggaag
    63541 aacttagaaa gttaagaccc aaaatgcaaa tgatatttca agacccatat agttcattaa
    63601 atccaagatt aaatgtggaa caaataatat ccgaggcagt tatagaacat aatttagtgc
    63661 caaagaatga aataaaagat aatatattaa atgttgtaga aagttgtggt ctttctaagt
    63721 accatttaaa tagaaaacca ggtgagtttt ctggaggtca aagacagaga ataggtatag
    63781 caagagcatt agcattaaat ccagaattta tagtttgtga tgaacctgtt tctgcactag
    63841 atgtttctat tcaatctcaa attataaatt tacttatgga tttacaggat aaatataaac
    63901 tttcatattt atttatatct catgatttga gtgtagtaaa tcatattgca catagagttt
    63961 gtgttatgta tctaggttct ataatagaaa ttgcaactaa aggagaactt tacttacatt
    64021 ctcaacatcc atatacaaaa gcacttatga gcgcagtacc aatacaagac cctacaatta
    64081 agaaaaatag aattatatta aaaggagaca taccatctcc aacaaatcca ccaagtggat
    64141 gtaagtttca tacaagatgc ccttattgta aggaaatatg caaaaaagag attccagttt
    64201 taaaagaaat atcaagaaat cattttgttg catgtcatct agtatagata tttcaaaaat
    64261 taaatttgat gtatataata agaataatag aataaactga aaattttttt gattaagatg
    64321 ggatttcatg ctgttaatat taagttagtt gatatataag tttaatctat aatattatta
    64381 ttgaaaagag aggtagttat gtcaatttta gtaacattgt tgggattatt ggtttgtaca
    64441 acgatttcaa ctgtatttag caaaaagtgg agtaatattc ctcttgcaat atatcaaatt
    64501 gtactaggga ttatattgtc tattttgcca tttaaattct cattttcatt taatccagaa
    64561 atatttgtaa tatgtatcat agctccatta ctattcagtg aaggacaaaa tgtatcgaga
    64621 aaagagctat tagagcttag aaaaccaatt ttgcttcttg catttggttt agttttaata
    64681 accgtgtttg caggtggaat ctttatacat tttcttattc ctgggatgcc attatctgtt
    64741 tcatttgctt tggcagcagt aatatctcca actgatttag tagcagtaaa atcaattaca
    64801 caaggattaa attttcctaa aaatataatg agtatacttg aaggtgaatc tttattaaat
    64861 gatgctgctg gagtagtagc atttaaggtt gcagtacttg ctacagttac aggagttttt
    64921 tcaatagaag aggcagggat tcaatttatg attacagctt ttggaggaat aatagtagga
    64981 agtattttag gatacataat tataaaaata agacttagtt tacacaagtg gaatttggaa
    65041 gaaattccaa tggtaatagt tatacaaata atgactccac tttttgtata ttttgtagca
    65101 gaagaaatag gagtatctgg aatcctagct gttgtaatgg cagggatagc tcatggaatt
    65161 gaaaaagagc atttacaaaa tacaactact aaattaagga taatatcaga caatacttgg
    65221 tatgttttgg agtatgtatt gaatggattt gtctttacac tgcttggttt tttgttacca
    65281 agtatatata caggacttag tagtaaaaat gaaaatatgg cattggaatt gacttttatt
    65341 tctactttaa ttgtacttat tttatttgtt ataagattta tatgggtata tttatggcat
    65401 aattctttta taaaacctaa aaagaatcct ttaaataatt tttttgtagg ttttttaggt
    65461 tttaaggatg aagaagttat aaaagagagt atttctaaat gtaaatactc actaatagtt
    65521 gctacttgtg gtgttcatgg tacatttaca ctagcaactg ccttatctat acctttttat
    65581 ttggctgata aagatgtatt tccaatgaga gatactgttt tatttatatc atcagaagtt
    65641 attcttataa gtttagtatt ggcaacaatt ttactgccaa gattattaaa aaataacgta
    65701 caagagtcag aacctttatt atctgataaa gaggcatata aactaatttt gaaagaagcc
    65761 attttaaaac ttagtaatga aaaagcacaa gaagtacaac cagttataca tgatttaaat
    65821 gaacagttgg tagactcaga aaaaggaata ctaaatactc cagataataa acttataagt
    65881 atgatgattg cagaagcaga ccaacaggga ttattagctg ttttgaaact attagaagat
    65941 ggtaaaattt ctgatagagc ctttaaattg tatagatttt atattacaaa atcaagaaaa
    66001 tctgttcaaa aatctatatt taaaattgta aaattaaaaa taagcatgtg gattataaat
    66061 agaaaagtaa agaaagaagt aaaagaaaaa attacaaaaa tagtatctga gaataaagaa
    66121 ataatcaaag agtttcacca tgcatatgca ctttctacta aaactgctat atcatttata
    66181 aataaaaata ctgatgaaga taacagacat gaggccttaa tagcaatcag attctataat
    66241 cgttatttga attggattac tagaaagatt caaaatgatg ataagtttga ggcaagagta
    66301 gaatactaca gagtaatggc gatacaaagt caaagagaca gtgttcaaca attagttgaa
    66361 actaagcaaa tatcaagaga agtggctaaa gagttattgg aaaatattct ctatgatgaa
    66421 atgatgatga tagaatcata aatataatgg aataaaattt gatttttgat gttaaaatta
    66481 atactaaaaa ataggaaata gttttcaata atttacttga catatatatt tatttataat
    66541 agaataaaac cataaattga atacaatata taagcattaa caagctggat tgttactggt
    66601 aagcaggcaa tacctaggtt aaggtataca ggtgaattgt atcttaattt aggtattttt
    66661 ttatgaataa gggaaattgt attttgggag agatattgat tatgattatt caacaaattt
    66721 ataataataa tgttgtatta gttttagatg aaaatataaa aaaagaatta atattaacag
    66781 gatgtggaat tgggtttcaa aaaaagaaag gtcaagaaat agataaaagt aaaattgaaa
    66841 gaacttttgt aatacaagat gaaagctttt tagataaaat tagtaaacta gcaagccagg
    66901 ttgatgagaa attttttgag gttagtactg aaataattgc ttatgcagag gagaacttga
    66961 atactaagct ttatgaatat atttatgtag cacttactga ccatattgca tttgcaatta
    67021 aaagatatca tgaaaacata actataaaga atgatttact acatgagata aaaagaatac
    67081 ataaaaaaga atatgagatt gggaaatggg ctgtagatta tataaataaa gaatttgatg
    67141 tgaaatttcc agtagatgaa gctggtttta tagcaatgca tatagtaaat tcaaactata
    67201 agggaagttc aaaggaatca ctattaatta ctaaaattgt aaaagacata ctaaatatta
    67261 taagatatta ttatagagtt gaatttaaag aagatgatat aaattatgat aggcttctga
    67321 ctcatttaaa attctttgca aaacgattag taaaaaaaga aaaaatcaat gatacaaata
    67381 atgaaattat agatataatt aaagtaaagt atgaaaagga ttatgattgt gcatataaaa
    67441 taaaaactca tgtagaaaag aattatgatt actatgtaag tcaagatgaa ctactttatt
    67501 taacattaca tatcaaaaga gttatatctg tactgaattt ataataaaga gtagtttaat
    67561 acagaatgta tagatttaat atattatgtt taattttaat atattattta aaatttaata
    67621 taaaataata attttaaaac attaagattg ttactgataa tgcaggcaag acttatgttt
    67681 ataaaaaaat tataatagtt tatttttaat tctgaaatta ggttaatata ggtttattta
    67741 tattaacata agtttaattt tagagttaag tatactaatg taacttttta taaacttaag
    67801 tcttttttat ttaaaaaaat ataaaataat aaggagagaa aagtaaatga aaaaagtatt
    67861 tggtgtttta caaaaagtag gaaaatctct aatgctacca gttgcattac ttcctgcagc
    67921 aggtatatta cttggcgtga gtaatgcttt agcaggagac gcattgttgg ctcattttcc
    67981 agctttggcg aatcctacat ttcaacttat aataagtata atggaaagtt caggtcaaat
    68041 tatatttaat aacttaccac ttatatttgc agttggggta gctgttggtt taactgaagg
    68101 agaaggtgtt gcagcattgg cagccatagt aggtttttta attctcaata catctatggg
    68161 tgttatggca ggtgttacag aagatatggt agacaattct atgtatgcaa atattgttgg
    68221 aataccaaca attcaaacag gggtatttgg tggcattatt atagggatag tggctgctta
    68281 tctttataaa aagttttata aaattgaatt acctcagtat ctaggttttt ttgcaggtaa
    68341 aagatttgta ccaatcgtta cttcattagt tgctatagca ataggaatta tattgtcttt
    68401 tatttggcct ccaatacaag agggattact tagtttctca aaaaatgtta tagaagcaaa
    68461 tcaaacacta gcagcattta tggaaggttc tttggaaaga ctattaatac catttggttt
    68521 gcatcatgtt ttccataatc cattttggta tcaatttgga gaatatgtaa ataaagctgg
    68581 tgagcttgta atgggtgacc aaaagatatt ctttgctcaa cttaaagatg gagtaaattt
    68641 cacagcaggt acttttatga caggtgcatt tccatttatg atgtttggtc ttccagcagc
    68701 agctcttgct atggtgcatg aagcaaagcc agaaaagaaa aaatttgtag caggtataat
    68761 ggcatcagca gcattaacct cttttttaac aggaataacc gagccaatag agtttgcgtt
    68821 tttatttgtt gcaccagtat tatttgcaat acattgttta ttttcaggtt tatcatacgc
    68881 aattatgcaa ttattaggag ttaaaattgg attcacattt tcaggaggat taatagattt
    68941 cttattattt ggtgttcttc ctaatagaac tccatggtac ttggctatct tggtaggtat
    69001 tggttttgca gtattatact atagtgtatt tagatttatg ataagaaaat ttaatttaaa
    69061 gacaccagga agagaagatg agtcagaaac taacgctgaa gtcattgaaa tgtctaagaa
    69121 tgaattagca tcagagattt tgattgcttt aggaaataaa gacaatttaa agagcctaga
    69181 tgcgtgtata acaagactta gattggttgt aaatgatata aataaagtag ataaagaaaa
    69241 attgaaatca cttggagcag caggtgttat ggttattggc aacaatgttc aagctatatt
    69301 tggaccaaaa tcggattcaa taaagtctga tataaaagat ataatagagg gaaaaacaaa
    69361 ggttgaaagt actgttacta aaaatgttga taaaaataaa gtgaaaaaaa atatggaagt
    69421 tgcaaatatg gaaaatgctt tagtagaaaa gattgattca ttaacaaatg gagagatatt
    69481 agatatagaa gaagtgccag attctgtatt ttcaagtaaa ctgatggggg atggatttgc
    69541 aattaaatct agtgatggat tgatttattc accagttgat ggaactattg gagttatatt
    69601 tccaactaag catgcaataa taataaaatc ccaaaaaagt ggagtagaaa tacttataca
    69661 tttagggata gaaactgtaa atttagaagg aaatggattt gaagtatttg tgaatgtagg
    69721 ggatgaagta aaagcgggag aaaaattagt taaaatggac ttagaatata ttgaaaaaaa
    69781 tggtctatca acaataagtc ctgtagtatt tacgaattta gaacaaaatc agaaattaag
    69841 cattaaaaaa ggagtaagca cagcaaaaga ggagaataga gtaagtatat taagagatga
    69901 tattgaaaaa ataggatagc tatatgatag tgtaactaaa agttatgtgg gatttaataa
    69961 atatataaat aggttcagtt aacatgcaat ttatttaata tatattttaa gtaatagaaa
    70021 tatatatcat tatattttga tatattttaa aacattaaaa aacttgaggt attagtaaga
    70081 taaatacact aatacttcaa gttttattaa aataaaattt acataaatta tctattcatc
    70141 taaaagagta atatccaaag gatttttatt gtttatataa tttttaggag ataccccgta
    70201 gtttttttta aatgccctta caaaactaga ataatcgttg aatccacatt tattacaaac
    70261 ttcactcata aggtgaccat tggttattag tgactttgcc aaaattaatc ttttttgaac
    70321 tatataattg tgtatagacg ttccagtatg agatttaaat tttctcataa gataatactt
    70381 acttataaaa aattcagacg atatgctatc tattgaaagc ttatcatcta tatttgaatt
    70441 tatatagttt aatactttct taatagtttc atcataaaaa acatcttctg ttgattccat
    70501 attttcattt tttaggaaaa ttctgtttaa gaatatcata agttgtataa ataatgcatt
    70561 tctcaatatt tcatttccaa attcagtatt ggattcggaa atatttattt gctcaataaa
    70621 actcttaata gattcaatat catgtatatt taaacgcaat ctattgcatt tattctctgt
    70681 agctatttta aaacattttt ctaagtcact actagcatca ctattttttt tcataaattc
    70741 tgggctaatc catattgcaa ttctctcata aaagacattt gaatctataa ctggtttatg
    70801 tatttcatta ttgctgataa agagtatatc ccaaggcttt agtttatagg gaataccatc
    70861 aacaaaataa gttacatttc catttataaa tataattatc ttattaaaat catgattatg
    70921 gtattcaaat tgtatatctt ttttatctct aatattaaat attttaaagt tatcgtttaa
    70981 gtacccgatt ttattatcca tagtttttat agaagacctt tcctcagtta tattatagaa
    71041 aattatagag cattttttgc aatgtttcaa gcatttttaa ccattcacat agtaaaaaat
    71101 tcttaatata atattatatg tagtgtaatt ttagcagaaa gagtttaaag ggaggaaagc
    71161 atttatgaaa ttaaatgata ttatacaggg gttggatata atcaatatca agggtgaatt
    71221 aaatatagat attaataatg tacaatatga ttcaagaaag gttacaaaag gtacattatt
    71281 tatatgtata aaaggatttg tgtcagatgg tcataagtat ataaaagatg ccatagaaaa
    71341 gggagcaagt gcatttcttg ttgaagaaga tgtagaaata aaaggatgta catttataaa
    71401 ggtaaaagat acaagaaaag atatggcaaa agtagctgat aatttttaca atcatccatc
    71461 tcaaaaattt aacgttatag gagttacagg tactaatgga aaaactagta taactactat
    71521 actaaatgag atattaactt taaataagaa taaagttgga cttataggaa ctataaaaat
    71581 ttttgatgga gaaaaagaca ttgtttccaa ttcaacaact ccagaaagta tagatttaca
    71641 atatcatttt aataatatgt tggataatgg atgtgactac tgtgcgatgg aagtttcatc
    71701 acattcattg gcattaaata gagttgatga aacagacttt aaactaggca tatttacaaa
    71761 tttaacacca gaccatttag attttcataa agatttagag gattatagaa aagcaaaaga
    71821 aaaattattt tttaagacta ctatggcaaa tataataaac atagatgatg aaggtggaaa
    71881 aaagatatat gaaaatataa aaggtataaa tgtaccttgt tacacttatg gtgttgatac
    71941 taaggctgat tttatggcta gagatataaa atcagattcg gatggagtat cttatagatt
    72001 aataacacct tcatatgaag aagtaatatt tataccagtt ccaggaatgt ttacagtata
    72061 caatacttta gcagttatag ctgcttgcta tgtacttggc atacctaaac caatatataa
    72121 agaaggcctt agactttcta atggagtatc aggaaggttt gaaacagttc caaatgataa
    72181 aggtataagc gtaattgtag actatgctca tactcctgac gcacttgaaa atgtactaaa
    72241 gacaactcaa caatttgcag aaggaaaaat aatttctgtg tttgggtgtg gtggagatag
    72301 agatactgaa aagagacctt tgatgggagc aattggacaa aaatattctg atttatgtat
    72361 tataacttct gataatccaa gaactgaaga accagaggca ataataaaag atatacttga
    72421 aggaattgac aagaaaaaag aaaattacca tgttgttgtt gatagggaac aagctatagc
    72481 agaagctata agtatggcaa aaaaggatga tgttgtaatt ataacaggta agggacatga
    72541 aacttatcaa attataggta aagttaagca tcattttgat gataaagaag ttgcaaatga
    72601 gtgtttaagt aagatgtagc ataaaaatta aacaaaatac aagaacaaaa atgtaatatc
    72661 aagcttcact ttgatattac atttttttat atgtaaataa ttgtaaaata gataatgcaa
    72721 taatataaac actaatatct atattaatta atttattgtt gtaataaagt ttataatttt
    72781 gtataattac agtaaatatg gaaaaataag tattattata aaatgtttac aataattgtc
    72841 ataagataat gattatacat acatagattc gagaaaggca ggaagcataa tgttaagtga
    72901 tgcaaaaaat aataggtatg gtttgtccat aattgctgtc ataagtatct tagtaattac
    72961 aatttcctgt ttgatttatg gttcaaggtt gaataagaca ttaggtgaag aaacgaatca
    73021 atatctttca gaaatagcaa atcaaagtgt caatgtgtta aaaaaacaaa ttaatggaga
    73081 tataaagtta ctagagtcaa tttcaatatt tattgaagga gaagagtctt ttgaagtaga
    73141 taatatcctt tctatactaa aaagacaagc tattaatagt tcatttaaaa gaatgggagt
    73201 tattttacca aatggtacag catacactac agatggttat atagaagact tctctaaaag
    73261 agattatttc ttaaaatcaa tgcaaggaga agtggttata acaggcaaat taaaagatgt
    73321 aatagaagat gataaaaata ccaatagtaa tattaatgta tattctgtac caatttataa
    73381 agataatgag gttaaaggag ttatatttgc cacacatagt acgaagttgt atgaaaaaat
    73441 actttctgta cctacattta atggaaaagg atattcttat ataactagaa gtaatggtga
    73501 tattgtttta aatccaagca gtaaaaacgc caactctact aaagaaaatt tattttctta
    73561 cattggcaga agtgattcaa tatcaaaatc tagtatcaac aatatgaagt caaatataaa
    73621 aaactttaaa tcaggttcat taagttatag tatgtttgga aaaggatatt atttaagtta
    73681 tgccccaata ggaatcaatg attggtatct tttttctgaa gttcctaaaa ctgctatatc
    73741 tgagaaatca tatgctatta taaaacttac attatcagca tgtgtagtct tgattataat
    73801 ctttacggca ttaacaattt atatactatt tatacataaa aagagtaagg aaaaattgga
    73861 agaatttgct tttaaagata acgttacagg tattggcaat tcaaataaat ttaatcttga
    73921 agggggaaaa tttttatcct ctcatgagaa aaagaatcta gttttaattt attttgatat
    73981 agataagttt aagctagtaa atgataggtt tgggtatgaa gaaggagata gagtattaaa
    74041 ggaaatagca gagataatta agaatatatt taaagagcaa tctgtatttt caagaatttc
    74101 aaatgacaat tttgctataa tatttgaaaa gaaaaataat agagagtcaa ttatagaaat
    74161 atgtgaactt atacgtaaaa aattgtcaat gataaaaaca agtttgggag tagaactcaa
    74221 tttaattcca tccataggtg tatattttgt tgaagaggga gaaacaaata tttctacctg
    74281 tcttgacaaa gctatgattg caaaaactac agtaaaaaga aaatatttaa aagtttatga
    74341 gatttatgct gaaaatttaa aagaaactct catagaagaa cgtgatattg aacaagaaat
    74401 gcatgatgct ttaaaaaatg aacaatttaa agtttattta cagcctaagg ttgatttatc
    74461 aactactaaa attgtaggtg ctgaagcact tgtacgttgg caacatccta aaaaaggtct
    74521 aataagtcca ggtgtattta ttcctatttt tgagaaaaat ggctttataa cagaattgga
    74581 tatgtttgta tttacccaag tttgtaaaaa ttttaaacgt tgggaaaatg aaaatttccc
    74641 cacatttcct atatcaataa acttatcaag agtacatctt gagaatccag gctttatttc
    74701 tgagcttgaa agaataacaa aagaatatga aattgaacca aatttaattg agattgaatt
    74761 gacagaaagt gcaatatttg acaatacaaa aatattattt aaaataatgc aaactttgaa
    74821 atctgttgga tttaaaattt ctatggatga ttttggttct ggttattctt ctttaaatat
    74881 gcttaaagat atgccaattg atgtattgaa attagatagg caattcttta taacagtggg
    74941 aaatgcaaaa aagagtcaaa ttgtagtttc aagtattgtt caaatggcaa agcaacttga
    75001 tataaaagtt gtttcagaag gtgttgaaac tgtagagcaa gcagagtttt taaggtttat
    75061 aggttgtgat atggcccaag gatttttatt tgctagacca atgccaatag aagaatatga
    75121 aaaattaata ttagatagat atagtttaaa tagaaaagaa acaaattaaa taaaattgtg
    75181 aattataaca taaaaattta taaaaaagtg aactttgtta atcaatgttc acttttttat
    75241 gtaaaaatat actattataa aaacataaat cattgttagg atattatttt aggaattatt
    75301 gggcaaagtt tattaatatg tagtattttt tacatttgaa tgtttttgag gtaagagtat
    75361 atgggattat agcaaaatac tttagatagt aataatagta atttctatag gtgtgttata
    75421 catattttaa aaaataaaaa tataatttat aaatttaaat gttatatatg taagtaattt
    75481 tattatatca aaaaaagagt ttttcataat catttataca aatcatttga aatactcttc
    75541 ttttattttt aaattctata taattattat aaggtaatat tgtattatta taatcttagt
    75601 tttttagtta ttatatataa tagtcaaagt gtatacgatt aatcatttta aacattaaat
    75661 ctaaataata ttacatctcc atctttaact atgtattctt taccttctaa tttaaccaac
    75721 cctttttctt tagcagcagc catagtacca tgctcaacca aatcattaaa ggctattgtt
    75781 tctgctctga taaatcctct ttctatatca gaatgtattt ttcctcctgc ctgaggagct
    75841 ttacttccaa cttttatagt ccatgctcta acttcttttg gaccagcagt taagaatgaa
    75901 attaaaccaa gtaaagcata agaagatttt ataagcttat caagccctga ttgttcaaga
    75961 cctaatgttt ctaaaaactc tttcttttct tcagcagaat caagttcaga aatttctgct
    76021 tctatttgtg cacatactac aacaacttcg gcatcttctg tttctgcaaa tgctttaact
    76081 tgttgaacat atttattatt ttctccatta tcagctaaat catcttctga tacatttgaa
    76141 gcatatatta caggcttaga tgtaagtaaa tccaatgaat ttacgaatgt ttgttcatct
    76201 tctgtaaatt ccatagttct tacacactta ctttcttcta aagtagacat tattgatttt
    76261 aataaatcaa gttcagaacc taaagtttta tcagccttag cagctttttg agttttatta
    76321 attcttcttt caagaatctc tatatctgag aaaattaatt ctaagtttat agtttctata
    76381 tcacgaagtg ggtctacaga accatctaca tgtacaacat tttcatcctc aaaacatcta
    76441 acaacatgaa ctattgcatc aacttctctt atatgtgata agaatttatt tcctaaacct
    76501 tctcctttag aagctcctct aactaaacct gctatatcac aaaattctat agcagtagga
    76561 actatttttt cagaattata taattcttgt agtttgttta atctttcatc tggaacagat
    76621 acaactccca catttgggtc tattgtacaa aaagggtaat ttgcagattc tgcacctgct
    76681 tgagttattg cgttaaatag tgtgctttta cctacatttg gtaaaccaac tatacctaat
    76741 ttcatattaa tcgtcctctc tcttattctt ttttatattc tttatataac tttaaacaat
    76801 caatctatag gattgcaaaa ttgttttaat tatagacata catattgatt atacatcata
    76861 tatatcaagc ttgtaaacta gactttaaag catattgatt taaataatat ttgtatttaa
    76921 tttaaataaa aatattgctt ttagaaaata ataaaaagga tataattaaa tagaaggttt
    76981 ttatatgtga gtatatgtaa ttaaaaaata aataaatttc aattttaaaa ttgatgttta
    77041 aataaaaata ggaggtacat atggttaata ctaaaaaaat aataaattta ggtaataata
    77101 ttaatcttac tttgataaaa acagaaaagt ttaaatcgaa tcttataagt ttatatgttc
    77161 agagactatt ggatgaaaaa gaaactacaa aaaatgctct aataccatca ataatagcaa
    77221 gtggaagctc taaatatcct agtgctagag caatatccaa taaattagat gatttatacg
    77281 gttcatctat gggagctgat gctgtaaaac gtggagaacg tcaagtattg agttttaaag
    77341 taattaacat aagtgaaaag tatttagatg agagtatatt tgaagaagta gtagaatttt
    77401 ttaatgaagt aataaataat acgcttgttg tagatggtgg gtttaaagaa gaatatttaa
    77461 atattgaaaa agagaattta agagaaaaga tacaatcaat aataaatgat aaaaaggaat
    77521 atgcgcaaga taaatgtatt gaggctatgt gtaaaggaga gagatatagt gtaagtgaat
    77581 ttggttatga agatgaaata gatagtataa cttcaagaga gttatatgaa cattataaaa
    77641 acatacttaa aacatcacct atagatatag ttgtagaagg taattttgat gaagataaag
    77701 ttgtagatat aataagtaaa aatctaaaat ttgaaagaga agaaattata aatataccaa
    77761 gagcagattt tataaaaaat gtggatgagg taaaagttat agatgaacaa atggaaataa
    77821 ctcaaggtaa gttagttatg ggttatagag caaatgtgga ttatgcagac atagataaat
    77881 attatgcttt agtagttggg agcaatgtct taggtggtgg tccacactct aaattatttg
    77941 ttaatgtaag agaaaaagaa agtttatgtt actatatatt ctcttcaata gagaagtata
    78001 aaagtatcat gtttatatca tctggtatag aaactaaaga ttatgataaa gctgtagaat
    78061 tgataaaaaa acaagtagaa agtgtaaaag caggtgatat atctgatgaa gaattagaaa
    78121 acagtaaact tgctttagtt aattctatga aatctataac tgataatata ggtggtatgt
    78181 ctgattttgt tttctctcaa tctatggcaa agacaaattc tgaagtacaa gatataatat
    78241 ctagtataga aaaggttaca aaaaaagata tagttgaagc tataaaaaat atagagcttg
    78301 atactgtata cttcttgaga aattaatgga ggagatgtct atggaaaaaa tagttaatga
    78361 tatattgaaa gaagaagttt attatgagaa attgcaaaat ggattagatg tatattttat
    78421 gcctaaaagg ggttttatga aaaagtatgc tattttagct actaattatg gttcaaatga
    78481 cttagaattt gtaccaattg gagaagataa gaaaattaga gtgaatgaag gtattgcaca
    78541 ttttctagag cataagatgt ttgagcaacc tgatggagga gatgcatttg ataaattttc
    78601 aaaattagga gtaaatgcaa atgcatttac taattttaca atgacagcct acttattttc
    78661 tgctactgaa aatttttatg agagtttaga acatctaatt gactatgttc aaacacctta
    78721 ttttacagat gaaaatgttg aaaaggaaaa aggtataata gcacaagaga taaaaatgta
    78781 caatgatgac cctgattgga atgtatactt taattgcttg aaggcaatgt atgtaaatta
    78841 tcctgctaga attgatatag caggtactgt tgatagtata tataagataa ctaaggaaga
    78901 gctatataaa tgttataata ctttctataa tccaggaaat atggctttat ttgttgttgg
    78961 tgacttagat gtagaaaaag ttatagatgt aactaaaaag tcaaacaatt acaaagtaga
    79021 taaactctct aagagtatag aaagatttta tccagaagaa ccagaaggtg taaaagaaaa
    79081 agaggttata gaaaaattcc ctatatctat gcctatgttc aacataggtt ttaaagatag
    79141 taatgttggt ttaaaaggga aagaactttt acgtaaagag attgtaactg atatacttgt
    79201 agggatgtta tttaaaaaag gaagtaagct ttatgaagat ttgtatatgc aaggtttaat
    79261 aaatgaaaat tttggagctg gattttcgtc tcaagtggat tatgctttta gcataatagc
    79321 tggagattct aaagagccta aaaaagtaaa agaaataatt cttgattata tagaaaaatc
    79381 aaaaaaagaa ggattatcaa aagaagagtt tgagagaact aaaaagaaaa aaatagggtc
    79441 atttattaag tgctttgatt ctataaactt tataggtaat agttttatat cttatgtttt
    79501 taaggatata aatctattgg agtatttaga tgttattaaa gatattactt ttgaggaagt
    79561 tgaagaaaga ttaaaagagc attttaaaga agagtattgt gttatttcta ttgtagagcc
    79621 taaatagaaa cttaatcata ataagaataa ctaattaagt attagtttat gtttttaaat
    79681 gtttattttg gtaaaacaaa tttataaaat atatttatat aaattgaggt aaaaatttaa
    79741 gagctataga taagaattag aagaggttga tgtatatatg catcaatctt tttgattaaa
    79801 aaatatctat gtacacttgt aactttagtc atgagagatt cagtttaaaa aattttatag
    79861 taattagata taatatagtg tagggagttg tagaataaat tttaatattt tttgtagaat
    79921 taacattaat gtgttatcat aatttagtat aattacaatt aattgtaatt atagcatatt
    79981 gctttggaaa tattttcaat taatacaaag aggtgacgtt atggatagag tagcatttac
    80041 actgtttggc atagacatca tgtggtatgg aatacttatg gcatgtggaa tgattttagg
    80101 aactttaata gcaataaaag aagcaaaaag agtgggaatt aaagaagatg atgtattaaa
    80161 tatagctata atagcaattc cagttggact aatctgtgca agaatctatt atgttgtatt
    80221 taattggagt tactatgctc aaaatatgtc tcaaattttt aattttagag gtggtggact
    80281 tgctatacat ggagggttaa ttggtggaat attagctggt tatatataca ctaagattaa
    80341 aaatattaac ttcttaaaaa tggctgatac agttatactt ggaatgcctt tagcacaagc
    80401 tattggaaga tggggaaatt ttattaatgg agaagcacat ggaggtgcta ccaatttacc
    80461 ttggggaata atggttgatg gagtaaaggt acatccaact tttttatatg agtctatatg
    80521 ggattttgga atatttatag ttttattact atttagaaaa aataaaaaat atgaaggtca
    80581 agttatagtt acttatataa ctttatattc aataggaaga ttttttatag agggattaag
    80641 aacagatagt ttaatgttag gaccacttag aatggcacaa gttataagtc taattggagt
    80701 tattggtggc ataattgcac atgtttattt atcaaagaaa aataaaaata atattagtga
    80761 agaatagttt aaaaagtgaa tttaagggag gtaactatgg aatttcatca tgtatcagtt
    80821 cttttaaatg agtgtataga gaatttaaat ataaagccag atggcgtgta tgttgactgt
    80881 actatgggag gagcaggaca ttctaaagaa atagtaaaaa aactgtcaga taagggatta
    80941 tttatagggt ttgaccaaga taaaaatgct ataagcacag caaaagagag attgagtgaa
    81001 tatgaaagta gagtgaagtt tgttcacagc aattttgaaa atataaaaga ggaattagaa
    81061 aaaataggag tttacaaaat tgatggtgta ttagctgatt taggagtttc atctcaccaa
    81121 cttgatgaag ctgatagagg attttcttat atgcaagatg caccacttga tatgagaatg
    81181 gatgttaggt gtgagttttc tgcatatgat gttgtaaata cttatacaga agatgaatta
    81241 actaagataa taaaagatta tggagaagat aactgggcta agcgtatagc taaatttata
    81301 gttgaagaaa gagcaaacaa accaatagaa actacaggag aattagtaga tgtaattaaa
    81361 aaagctatcc ctaagaaagc tagaatagat ggacctcacc cagctaaaag aacttttcag
    81421 gcaatacgaa tagaagtaaa taatgaactt ggagtaatta caaagatgat aaatgatgct
    81481 tcttctataa tgaatgaagg tggaagaata tgtataataa cattccattc attagaagat
    81541 agaatagtga aaaatgcatt taagcattta gcatcagatt gtatatgtcc acaacattta
    81601 cctatatgcc aatgtgataa agaatctgag gtgaaaataa taactagaaa acctatatta
    81661 cctagtgagg aagaaataga agtaaatcca cgttcaagaa gtgctaagct aagagtagca
    81721 gaaaaaatat aagtagttat aaggctaaag aataatctta tatataaaat aagattatta
    81781 taattaatta aaaagtatta tatattttgt tgcactggta aagcaattaa aaaacaatta
    81841 acactataag aaatgttctt tgttattgag atatataatg aaaatttaga tataaaaata
    81901 tagatataga tggtattaag taatataatg gagaacttag gaatcagaga gtagatagtt
    81961 agttacatat tatatttatg gaagaaatta tctaaaaata ctagaagtga taaaaataaa
    82021 tagaaattaa tatctaacct atagattcat ataaaagtat aagtgaattt ataggttttt
    82081 gttgtgtata gaaaaaaata caattagatt gtgtaatagt gtattaattt agcgatgaaa
    82141 ttttagagga agaaatatga ttaataacaa atatagtata gaggaaaatt taaactatat
    82201 actatcgggg tgagaatttg aaaaaaaatt ataaaataca taatctaaat gaatataaaa
    82261 ataaaaaaaa gaataattat aaaaagagaa ataagaaaaa aataggagct aaatgcattt
    82321 tactgattgc aactttttca gtaataatat tgaatctatg tggttattca aaaatctcgg
    82381 aaatgaagta cgatatctat tatttacaaa aagatttaag aaaaaaagag atagtattag
    82441 aggaactaaa ggcaaaagaa actgaaaaaa catcaataaa agaaattgag ctaaaggcta
    82501 aggaattgct caatatggac tatccaaaag atggacaaat aaagtatata gatgtggata
    82561 actaggggga atagatttga gaaaagtaaa gaggataagt aagaaaaggc tggtactcgt
    82621 tcttattcta gcatgtgcat tgtttttttg ccttgttatt aggactgggt atctacaact
    82681 tatgaaggga aattggttga gtacaaaagc actagaacaa caaacaagag atataccaat
    82741 agaacccaaa agaggaacta tatatgatag aaatatgaaa gagttagcag taagtgtaac
    82801 taagtatact gtttggtgta aacctgtgga agtagaagat aaaaaagaag cagccgaaaa
    82861 ggtagctgag attttggatg aagattataa agatatttac gctttaataa gcaaaaaaaa
    82921 tatggctctt gtaaaagtaa aaagatggat agatgatgat aaggcaagtc aaataagaga
    82981 tgctaaatta agtggaatat gggtggcaga agataatcaa agatattacc catatgggaa
    83041 ttttgcacca tatgtgttag gacatacttc ttcagatgca acaggtatat ctggtgtaga
    83101 aatgcaatat gataaaaagt taaaaggaaa gcctggaaaa cttatagtaa gtactgatgc
    83161 ttcaggaaga gaaataccac aaggaatgga aaaatattat gagcctgtac aaggtaatgg
    83221 tcttgtgttg tctatagatg aagtaattca acactatact gaaaaagcag tacagaaagc
    83281 ttatgagtta aataatgcaa aaaaggttac tgcaatagct atgaacccta aaacaggtga
    83341 tatacttgct ttagcatcaa aaccagatta tgaccctaat gactcaagaa cgccaatata
    83401 tccatattat caagaagaac ttgaaaaata taatgataaa gataaaataa aaggctatta
    83461 tcaaatgtgg agaaatccag cagtaagtga tacatatgaa ccaggttcta catttaagct
    83521 tattacatct tccagtgctc ttgaagaagg agtaattaaa gatggagaaa aattcacatg
    83581 tacaggtagt gtaactgttg gaggaagaaa gattaaatgt tggagacatt acagacctca
    83641 tggaacacaa gaatttaaac aagcagtaca aaactcttgc aacccagtat ttgttgagtt
    83701 gggaagcaga ttaggtgttg gaaaaatgta tgattatata gaatcatttg gtcttatgga
    83761 taagacaggt atagatttac caggagaagc taagggaata ctgtataacg aaaaaaatgt
    83821 aggaccagtt gaattggcta caatatcatt tggtcaatct atatcagtaa ctccaataca
    83881 acttataaca gctatatcat caatagctaa tggtggagat ttaatgcaac caagagtggt
    83941 aaaatcttat actgacaata aaggaaatat aactgaaaca gtaaaaccaa aaaaagtaag
    84001 aagtgttata tctaaagaaa catctaaaaa aatgttagaa attgcagaat cagttgtaac
    84061 tgaaggtgga ggaaagatag catatatacc aggttataga cttggtggta aaacaggtac
    84121 agctcaaaaa gttatagatg gtaagtatgc tccaggaaaa tatatatgtt catttgttgg
    84181 aatagcacct tgtgatgacc cgcaaatagt agttcttgct attgttgacg aaccaacagg
    84241 agtaagtgca tttggtagta ctacagcagg accaatagtg aaagaaataa tgaatgactc
    84301 tttaaaatat ttgggagtta aaccagtata taaagaagaa gaaaaagctg agtatgaaaa
    84361 gaagcaagtt aaagtgccag atgtaagaaa tctaaaaata ggagatgctg taaaggcatt
    84421 ggaggatgcg aagttaaaac cagacttaga tgcagatata gaacttccag aagatacaaa
    84481 ggtaaaggat atattcccaa aacctggagt aaaagttaat gaagactcaa gtataaccct
    84541 atattttgaa aactaaaaag taacagataa ttaacatata cccataaaca tcttcaaaaa
    84601 aacagaaata tagtatataa ttataaagtt aagagttatc gtaggcaatg tatatataac
    84661 ggtatacatt gccttttgtt gctaagagaa tatagctaag gttttaaagg aggagtttaa
    84721 taatggagtg cttaacaata aaagagttag tacttgcaac taatgggaag ttaatatatg
    84781 gagattataa tgactgtgta agtgatatag ttatagatag cagggaggca aatgcccaaa
    84841 atgcatttgt agccatagtt ggagaaaatt tagatgggca tacatttatg aagtctgcat
    84901 atgatagtgg atgtaaaact tttataaaaa atgaaagtaa tggaataaaa ttagaaagtt
    84961 cggatataaa tttaatagag gtaaaagata catctttggc tctaggagat atttcaaaat
    85021 actataaaga aaaatttgat attccattta taggtgttac aggtagtgtg ggaaaaacta
    85081 ctactagaga tatgatttac tctgccatat ctgcaaagtt aaatatacta aaaaatgaaa
    85141 aaaacttaaa taatcatttt ggagttccat tgactttatt taatctaaat aaagaacatg
    85201 agtgtgctgt aatagagatg ggtatgtctg gattcaatga aataaaatat ttagtggata
    85261 tagtaaatcc taaaattgca gtcatatcga atataggatt atcacatgta gaaaaactag
    85321 gttcacaaga gggtattttg aaagcaaaga tggagattac ttcaaacttt gatgaaacaa
    85381 atactttaat tgtaaatgga gatgataagt ttttatctac attaaaagaa aaagaacatg
    85441 tatataagtt aaaaacattt ggctttaata aaaacaatga tgtttattgt gaaagttata
    85501 ctatggaaga ggatagttta acatttatat gtgtaataaa tgggaaaaaa gaggaaatat
    85561 ttataccaac aataggagaa cataatatat ataatgcaat gtctgctata ttagttggtt
    85621 tgtgtctaaa tatttcttta gattatataa aaaaaggttt aaaaaatttt aaagggacaa
    85681 aaatgagatt agacataata aaaaatgaaa aattaacaat aattaatgat tcatataatg
    85741 ctagtccaga ttctatggat gctgctttaa agatacttgg aagatataaa ggtagaaggg
    85801 tagcgatact tggagatata ctagaaatgg gtgaaatttc agagtatggt catagattag
    85861 ttggaaaatc atctatgaat aatactgata ttataataac tataggtgaa aattcagtgt
    85921 ttattggaga agaagcaaaa caattaggat tcaattcagc caatatatat cactttgaga
    85981 atagagaaga tgtgtttaac aagttaaatg aattagtaaa aacaggagac actattttag
    86041 taaaaggctc aagaggcatg agattagaaa aaatagtcga atatctaaat aaataagctt
    86101 aaactaatga gttaattgac aagggagagg agttttatga tgttaggaat aacagagctt
    86161 acgtacaccg cattgattgc gtttttaata gtaattataa taggaccaat atttatacca
    86221 atgcttagaa aatttaaatt tggtcagaca gttagagatg atggacccca aacacatcta
    86281 gctaaaaacg gaacacctac tatgggtgga attataatga ttgttgcaat tctaataact
    86341 ggacttacaa gagtaaaagt tagtcatgat atggcagtag gtcttatatg tattgcaggt
    86401 tttggtttta ttggcttttt agatgatttt ataaaaataa agctaaaaag atcacttgga
    86461 ctaaaagcat atcaaaaaat aatccttcaa gttgcattat ctttttatgt tgctttttat
    86521 cagtatacat catcatcaag tgcttcacaa cttatgatac catttacaga ttttgtgata
    86581 aatgttggaa tattatatat accaataatg atgtttataa tcgtagcaat agtaaatgct
    86641 gtaaacttga ctgatggatt agatggatta gcatcaggag taacattaat agtttctgta
    86701 ttctttatgc tatttgcaag ttctattgct ggaaatacag aagtcgcagt tttagcagca
    86761 gcaactgttg gagcatgttt aggtttctta ggatttaatt catatccggc tagagtgttt
    86821 atgggcgaca caggttcaat ggcattagga ggtgcagttg tagcattttc agttctaact
    86881 aactcagtgc ttataatacc tataattgga ggaatttatt ttgcggaggc tctatcagtt
    86941 ttgattcaag ttggatattt taaggcaaca agaaagagat tctttaagat ggctccaata
    87001 caccaccact ttgaacagtg tggttggcca gaaacaagag ttgtatttat tttttggatt
    87061 ataacagttg ttttagcttg gataagtata atagcagtat tttagggcta gggggaatga
    87121 aaaatggact taaaaggaaa aaaagtttta ttagtaggac ttgcaaaaac tggtatatct
    87181 acaattaaac atttagataa attgggtgca agtgtaatcg taaatgatat aaaagatgaa
    87241 aataaattga gaaatatact tgatgaattg aaatctataa atgacataaa atacatatta
    87301 ggacatcacc cagaagatgt agatgatata gatatggttg tagtgtctcc aggtgtacca
    87361 ctagacctac cattcatatt gaaattaaaa aattcaggca agtatataat tggtgaggta
    87421 gaattagctt ttaaactatc gaataatcca atttttatag ggattacagg tacaaatgga
    87481 aagacaacta caactagttt ggtaggagaa attttttcaa gagcaaaacg agatacatat
    87541 gtagttggta atataggaaa tcctgtaata gatactatag aaacttcaag tgaagagtct
    87601 gtgttagtaa ctgaactaag cagttttcag cttgagagta tagatgaatt tagacctaaa
    87661 gtaagcgcaa tattaaatat tacagaggac catttaaata gacaccatac tatggaaaaa
    87721 tacatagaag caaaggcaaa tatatttatg aaccaaactg ttgaggattt ctgtgtatta
    87781 aactatgatg atgagatagt aaaatcttta gctgataaat gcaatgccaa agtaatttac
    87841 ttttcaagaa ctaaaaaagt aaatggagga gtatatttag aaaataatga cataataata
    87901 gatatagatg ataaaatcaa atttctaaat aaagatgatg taagtttacc tggaggtcat
    87961 aacttagaaa attgtatggc agctatagca atagcatatg tctgcaaaat tgatttagaa
    88021 gttataaagg atgtacttat gacatttaaa ggagtggaac acagacaaga gtttgtaaga
    88081 aatttagaca atgtaatata tgtaaatgat tcaaagggaa ctaatccaga ttccactata
    88141 aaagctatac agtcttatga tagacctata atacttatag caggggggat ggataaggga
    88201 agtaattttg atgaacttct tgaaactgca aagtcatatg ttaaatcatt ggtcttatta
    88261 ggagaaactg catcaaatat agaaaattgt gctaaaaata aaggatttaa tgacatacat
    88321 atagtaaaag atatggaaga agcagttaag acttcttatg aaatttcaaa aagtggagat
    88381 atagtgcttc tttcaccagc ttgtgcaagt tgggatatgt atgaaagttt tgaagtaaga
    88441 ggaaaagact tcaaagataa tgtaaacaat ttaaaataat ctcttacctc taaacttact
    88501 taaatggaag aatggaggta gattagttgg atgcctaaag aaaatttgaa aaaacaaatt
    88561 gacataagga tgaagactga atttgatgga gtagtgtttt acactactat gcttttagta
    88621 tttgtaggaa tagttatggt gtttagtgca agttttatac aatcttcatt taaacacaat
    88681 gatgcgtact attttctaaa aaagaatgta atatatgctg tacttggatt tatagttatg
    88741 attattacat caagaataga ttatagtttt tggaagaaaa atgctacagc aattggagct
    88801 atagcagtag tattattatt gttagttttg acccctttag ggatagaagc aaacggagca
    88861 aaaagatggt tgggaatagg agctttaaca tttcaacctg cagaaatcgc aaagtttgct
    88921 acaataatat taactgcaaa attaattgaa aaaaattatg ataaaataaa atctttgaca
    88981 aaaggtgttg taccacttct cgtagttcca gggatatttt ttgcgttgat aatacttcag
    89041 ccaaatttgt caacagcagg tactgtaatt ctagttactt ttgtaatgat atttgttgct
    89101 ggaatggata tgaaaattgt atttgctatg atagggagtg gggctgcttt atttgctgca
    89161 ttagtaattg ctgaacccta tagacttagt cgtgttacat catttttaga cccttttcaa
    89221 gaccctcttg gtaaggggta tcaggtaata caaggtctat atgcactagg ttcaggtgga
    89281 ttgtttggtc taggcttagg aaagagtaaa caaaagtatt tttatatacc agagccacaa
    89341 aatgacttta tatttgctat aataggagaa gagttaggat tgattggttg tataatagtt
    89401 attatgctat ttgtagtttt agtctacaga tgtgtaagaa tagctttgaa gacttctaat
    89461 gtatttgctt gtatggttgt tataggtata ggtgcacaaa ttggaataca agcagcttta
    89521 aatatagcag ttgcgacatc atcaatgcca gctacaggtg tagcactacc atttataagt
    89581 tatggaggaa cttcactcac catctttatg ggagctgtag gaatagtgtt aaacatctca
    89641 aagcatgtca aaattaatta acaggagatg atgatttgat gaaagtttta ttatcaggcg
    89701 gtggaactgg agggcacgtg tatccagcta tagctattgc aaacaaaata agagatgagc
    89761 atccagacgc tgagataatt tttgttggga cggaaaaggg aatagagtca gaaatagttc
    89821 ctaagtatgg atttgaatta aagactgtaa cagttcaagg ctttaagcga aaaatagatt
    89881 tcgataatgt aaaaagagtt tttaagcttt ttaaaggttt ggagcaatca agaaagattg
    89941 ttaagaaatt taaaccagat atagttatag gaactggtgg atatgttagt ggacctgttt
    90001 tatttaatgc atctatgggg aaaataccag ccataataca tgagcaaaat tcttttcctg
    90061 gtgtaactaa taagatttta tctaaaacag ttacaaaggt acttacaagt tttgaagatt
    90121 cgcataaaag atttccagag gcagcagaag ataaattagt ttttacagga aatccagtta
    90181 gaaaagaaat tttattatca agaaaaaata ttgcaagaaa gaatctatca ataagtgacg
    90241 aaaaaagaat ggtactttgc tatggtggta gtggaggttc aagaaaaata aatgatgcaa
    90301 tgagacttgt aataaagaat atggtaaatg aagatatagc ttttatattt gctacaggca
    90361 aaagctatta tgatgaattt atgggaagta ttagcgatat aaatcttaaa ccataccaaa
    90421 aagttgtgcc ttacttagaa gatatggcaa atgcacttgc agcaagtgat ttggttatag
    90481 gtagcgcagg agcaatttca ttagctgaga taacggctct tggaaagcca tctataataa
    90541 tacctaaagc ttatactgct gaaaatcatc aagagtataa tgccaagagt atagaaaaac
    90601 aaggggcagg aattgctatt ttagaaaaaa atctaactcc agagagccta aatacagcag
    90661 tatttaaact tttaggagat agagaacttt tggttgatat ggcaaatgca agtaaaacta
    90721 taggtaagcc tgaagccata gaccttatat atgatgagat aatgaaagta tacaattcaa
    90781 cacaaaagtc aacttctaaa aaaacaaaaa aagaaaaagt tattaaggaa gtaaaagaaa
    90841 taaaaaaaga aacgactcca agtatagaag gtcaagctaa ggtaatagga ataaagaaaa
    90901 gataagtaaa tatttacccc taatttataa tatattagat tttctaaatt attataaatt
    90961 aggggtttat ttgtatgtat acagatttaa atatataaat agtatgtagt atgctatgtt
    91021 tattttgatg aaatattgat ttatttataa aatttaatac cttatttgga tgttatgtaa
    91081 taaaatgcaa aaatttgact ttaaatatta taattttttt gtaggaaaaa caagctctaa
    91141 aggtgtattt attaaccaaa ggggttgatt atatttgaaa aaaagaagaa agttaaatac
    91201 gaataatgtc atgatagttt tagtttttat acttatgtta tctttttgta tatgtataat
    91261 tgtaaaaagt gatttatttg atgtaaagaa aatagatgta attggaaata agagggtgac
    91321 caaatcaaat ataatgaaag aactaaatgt aaatctaaat gaaaatattt ttgcatacaa
    91381 ttttaaagat atgaaaaata aacttataaa aaatccatat atagaaaatg tggaaataaa
    91441 aagaaaactt cccaataaga taattataag tttaaaagaa aaagaaatat ttgcagtatt
    91501 aaaagatgaa gataattatt gttatataga taaaaaagga aatttattgg aagaactaag
    91561 aggttctaat gaaagtaaaa aagacttgat agtagatgta gattatagca ttgatgataa
    91621 taaatctata aaatttaaaa attataaaac taaggagaat gtatttaaaa ctttaaatta
    91681 tttaaaagaa gaaggaattt atagaaaaat aaattatgta aacttaaaaa aagaaagcaa
    91741 tatagaaatg cttacaagaa gtaatataaa aatcttactt tctaatgatg ataacttaga
    91801 ttataatata tctagggtaa gtaaaattct gattgacctg caaaataaaa atactaatgg
    91861 agggactata aatcttaact atggaaagtt ggcagtgtat agtccagaag gatagacaat
    91921 tgtaaggaag tgatatattg aaaaaaagat ttataaataa aagtataatc ataggaagta
    91981 ttgtattggg tgttcttgtt agtttacaac ttaaaattat taatatagaa aatagaggga
    92041 tgacaacttt taaaagagga gagcaactta cagaagaact taaatcttta aaaaaagaaa
    92101 aagagaatct acaatcagaa gttaatagtg tgaagaaaga tatagagatt tatagaggca
    92161 aaaacaatga aggagaacaa gacatactta agtcagaaat agaaaaatat gaaacattag
    92221 ctggatatac agatgttgaa ggaacaggca taaaaattga gataaattct acaaaagact
    92281 taggaaactc tgataaaaat gatagtatac tatataatta tgatttatta ttatcaatga
    92341 taaataaatt aaattcagca caagcaagtg ccatatctat aaacgaccag agaatagttt
    92401 ctaatacata tatgtattta aaagaagaca agctttatat gaatgacact gtcataaaag
    92461 aaccaattat aataaaagca attggtgatt ctgatacatt ggcttcggca cttaatataa
    92521 agtatggtat tgtctgggaa atagaaacat attataatgc caaagtaaaa gtgaccaaag
    92581 aagacaatat agaaataaat ggtcatagtg atataaacaa agcagagggg aaataatagt
    92641 atgaaaaaga aaatagttat attaggagca ttcatattag gcgttttcat atcaacttat
    92701 ataaaatcac tagacccaaa tagagtatac ataacattag gtcaaactga aagtattgaa
    92761 agtgaggtag aaaatacaaa aaaagaaata aaaaacttaa aagaaactct taaaaagaac
    92821 aaaaaaactt tgaatgaata taaagaagca aaaaagaatg gaaatgaatc tatccaaaca
    92881 cttatgcaaa aagagcttga agaagttaaa gaattaagtg gttattcaac tgttacaggg
    92941 ccaggcataa ctattactat gagggatagt gagagagagt taaaagacgg acagaatcca
    93001 aacgatttga ttatacatga tatagatatt ttgagggttt taaatgattt aaaaaaggct
    93061 ggagcaagag caatttctat aaatggagaa agagtactag caacaagtaa gataaaatgt
    93121 tcaggagcta ctattacagt aaatgatact acatatggac aaccatttat aataaaagct
    93181 ataggagata tagatttatt gagtgcagct ataaactctc cagaatcata tgctaaatct
    93241 cttaaggata tatatggaat taatataaaa gttcaggaaa ataatggaaa taaagtgaat
    93301 ttatttgcaa aataaaacga aaaatacaaa aaaataaaga aaaagtaaaa aaaattaata
    93361 aaaaggattg taatgtagtt gtgtttcaaa gtaaaataaa gaggtgaaga tttatgttat
    93421 gggttttagg aggattagca tttggtgtag tagtaggtta ctatattcca tttacatatt
    93481 ctatggatta ttccatatat atgtcagtag ctattcttgc aataatggat tctatttttg
    93541 gagcgataaa atccaatttt gaagataact atgataatgt aatttttatt actggtttca
    93601 taggtaatgc tatattggct atattattga cttatatagg agaaaagtta ggagtttctc
    93661 tttactatgt tgctgtatta gtttttggta tgagattgtt taataactta tctataatta
    93721 gacgatatat tattagtaat attatgaaca aaaaacgtaa aaattaaaga gcatttaata
    93781 tatacgtaaa ttattttata cttaaagagt aatatttgac aatataagta agaaatttaa
    93841 aaaggaaaat ttacgttttt gtggaatatg ttacttaaag gttaacaaaa taaaagaaga
    93901 aaatgatata attaatacat acgataactt tgaatcatga gagaaggggg tttatgatgc
    93961 taaactttga cgtagaatta gaagaatgtg cacaaattaa agtaataggt gtaggtggcg
    94021 gtggaaacaa tgctgtcaat agaatggtag aagctcaact aaaaggcgtt gaatttatat
    94081 cagtaaatac agataaacaa gctttatata catcaaaagc tgaatataaa gttcaaatag
    94141 gagaaaaatt aactagagga cttggagcag gagcaaatcc agaagtaggg aaaagagctg
    94201 ctgaggagag taaagatgaa atagtaaagt tacttcaagg agctgatatg gtctttgtaa
    94261 cagccggaat gggcggtggt actggtacag gagctgctcc agtggttgca ggcttggcaa
    94321 aagaaatggg gatacttaca gtaggtgtag ttaccaaacc ttttgcattt gaaggtaaga
    94381 taagaatgaa gaacgctgaa ggtggaatag cagaacttaa atcaaaagta gatactctta
    94441 taacgatacc aaatgataga cttttacaga tagtacaaaa aaatacatca atgttagatg
    94501 cttttgcagt tgcagatgat gtgttaaaac aaggtataca atcaatttca gaccttatag
    94561 cagttgaagg gttaataaac ttggactttg cagatgttac tactataatg aaagacaagg
    94621 gacttgctca tatgggtata ggtagtgcta gtggtgaaac tagagcaatt gatgctgcaa
    94681 gacaagctat tcaatctcca cttctagaaa catcaataca aggagctaaa ggagtacttc
    94741 ttaatgttac aggtggtcct aacttaggat tatttgaagt taatgaggca tcaactttag
    94801 ttatggaatc ctgtgaccca gaagcaaatg ttatatttgg agcttctata aaagaagatt
    94861 taggtgatga aataatgatt acagtaatcg cgacaggatt tgaaggatta caaaatggtg
    94921 ccttagattt agatactaaa cctaaatcaa gcatacgttc ttcattaaat acaacagtaa
    94981 aacaagctgt aaaagaaatt gaagaagaag taattgctga ggaaaaaata gagcctccta
    95041 aaaaggcaag tataattgaa gaagatgatg atgagagcat ggagatacct acattcctaa
    95101 gaagaagaag ataatatgta agcttataaa gaagtcaata gacttctttt tattttgctc
    95161 aatttagata gttagtttgt ggctagactt aaaaaaattt ttaaatattg ataaaattta
    95221 aaaagtaagt ttttagtatg aattaaatct aataggtata gaaaaaaaat atagttatag
    95281 gagttagtat agttttttat gattagacaa gagtaatctt atttgttaat attaaatctg
    95341 aattaaatgt aaacatttga ggaaaaagac aaattaaagt ctgaaagggg attaagcatg
    95401 attaaactaa aaaaggttct ttctttagtt atggtaggga ttctatcagt atcaattctt
    95461 gcaggatgtt caaaaaaaga agattctaaa ggcaaagagg gtaaagaaaa attagttgta
    95521 tggtctgcac cattaacaga ccatgatgca gatgcatgga agccgatatt tgaaaaattt
    95581 gaaaaagaaa ataactgtga aattgaattt cagatagtac catgggataa ctacgcagaa
    95641 aaatatgcaa cagcaataag tgcaggtgaa ggtccagata taggatacat gtatgcagag
    95701 atgtttcctc aatttataga aatgggtgcg gtagaagatt taacacctta tttagaaaaa
    95761 tcaggaactt cagataatta tctatactta gatgatgcaa aaatgatggg tggtatttat
    95821 ggtttaccta tagaagctgc taatccaggc gtattgtact acaataaaga tatactagaa
    95881 aaattaggag aaaaacctcc aaaaacatgg gatgatttta aaagaatctg tgaaaaagca
    95941 acaaaagata ctgatggaga tggaaaaatc gaccagtggg gtcttgcaca aggttggggt
    96001 tctaaagtat ttggaagctt aaactggaac tggtatccgt atttatggca agctggtgga
    96061 gatatatata atgatgattt aaaatcagtt aaatttaata atgcagaagg cttgaaagct
    96121 gcaaacttta taaatgatat gaaaaagtat attccagaag atgctttatc aaaagatagt
    96181 aatgaaatga ttgaaagtgt atttggacca ggaaaagctg catttactat aatgctatcc
    96241 tctgctgcta caagtacatt tgataaatca ttcccggatt taaactgggg atatgtaaca
    96301 tcattagaag ataagacagt tggaagtttt gcttcagtag atgatttaac attaatgtca
    96361 gcatgtaaaa ataaagaact tggatttaaa cttcttgagt atatgctaag caaagaatca
    96421 atggaagctt tccacaaaga aatacctaga gctccagttg ctaaaggtga aaaataccaa
    96481 ggagacccaa gatttgaaga aatggtgaca agagataaac atatgtatag accattgaca
    96541 gttggacctc atggagtaga gatatatgaa tatttatgga aacaacttca aatgatgata
    96601 gctggagaca taactccaga acaagcacta aatgaatctg caaaatatgc aaatggatta
    96661 ctagcacaat aatttatatt tgaatttaca gactatataa tttgatttta tataagtaaa
    96721 agatttactt gatatattat taaattatgt agtctttttt tgtaaatata ccaaaagtag
    96781 agcgaataac ttctacctag atatattgga ttctacaaaa aagtctattt gtttggcatt
    96841 aaaaaaatat cttttgacga cataaagtat aagacatata tcattgttct ttttaaatag
    96901 aatttgtttt ggattcgacc atcaaaagtg acataaaatt ttgtacatac tatcttataa
    96961 tatgtttaaa ggttttaagg ggggatacat ggtgtatatt gagtactatg ttatagaaaa
    97021 cctagtaata aattatatta ttataagttg tacttctatt cttactaaaa gatataatcc
    97081 taaaaaaaat aagttattgg gggctagttt aggaggtgcc tattctgttg cttatttgta
    97141 tccatcaatg aatatactat ttactgtacc atttaaaata ataatcatga gtataataac
    97201 tttaatttca tttagaagta agagcaaaaa agattatgtt catgtagcgt tagtttttta
    97261 tctagtaaac atttttattt ctggaagcac ttattttata atttatttta caggaataag
    97321 tcacttaaaa atatctttct taattgcatg tacttatgtg agttgtgaat tattaaaata
    97381 catttataaa gatttgaaaa tgcttaagta cattaaggag cttacaaaga ctataaatat
    97441 aaacttacta gaaaaaagtt tttgctgtaa ggctttagta gatagtggta atttgttaaa
    97501 agacccaata agtcaaagtg atgtaataat agttaagtct agtgtgttag aaggatttat
    97561 tccagaaaaa ctattaaata ttaattatga agatatagat atgaagaaag cacaagaaat
    97621 tataggaagt ttagatgata aaacttcagg tagagttaga ataatacctt ataaacatgc
    97681 tggaagtaat aagacaagca taataatagg cttaaaagct gactatatag aagtagatga
    97741 gcaaaagata ggaaatataa tacttggtat ttcaaatttt aatgatagag aatatggagc
    97801 aataatgaat ccaaatatat tgcctggatt ttaggtgaaa atttaagggg gacaaggaat
    97861 gttacgattg aaagaaagaa taataagctt tattactatg ttaggtatta aattaaaaat
    97921 aataaagcca aagggaatat actatatggg aggggcaaat atacttcctc ctccattgaa
    97981 accagaagaa gaaatggagc ttttacaaaa gctagaaaca gatgaaagtg taaagtcaat
    98041 tttaatagaa agaaatctta gattagtagt ctacatatcg agaaaatttg aaaatacagg
    98101 tatagatgta gaagatttga tttcaatagg aactataggt ttgataaaag cagttaatac
    98161 ttttaaactg aataaaaata taaaattggc aacttatgct tcgagatgta tagaaaatga
    98221 aatattaatg tacttaagaa aaaataataa aaagaaaaca gaagtatcat ttgatgaacc
    98281 tttaaatata gatttagatg gaaacgaact tttattatct gatgtattgg gtacagaaaa
    98341 tgatgaaata tacaaaataa ttgaagaaga aatagataga gatttgttgg ttatggcact
    98401 tgacagatta agtgacagag aaaagcaaat tatggaatta agatttggtc ttattgataa
    98461 aggtattgaa aaaacacaaa aagaagttgc tggaatgctt ggaatatcac agtcctacat
    98521 atctagactt gaaaagaaaa taatctcaag gttgcaaaaa gaaatgaaaa aatttgtata
    98581 atgctaaatt aattcttctt aaaaagtata aaaaaattgg aaatgtaaat aatttcaata
    98641 gatgaatact tttgagagag gactgaaaat atatgcaagt taataaggtt gaaatatgtg
    98701 gtgttaatac atcagaactt ccagttctta aaaataagca aatgaaggaa cttttattac
    98761 aaataaaaaa tggtgatgaa gaagccagac aacagtttgt aagagggaat ttaagattag
    98821 tcttgagtgt aataaaaaag tttaacaaca gaggagaaaa tatagacgat ttatttcaaa
    98881 taggttgtat tggtcttata aaagcgatag ataactttga tttaagccaa aatgtaaggt
    98941 tttcaactta tgctgttcct atgattatag gagaaattag aaggtatcta agagacaata
    99001 acccaattag agtaagtagg tcattaaaag atatagcata taaggcacta caagttagag
    99061 aaagattaat tagaacaaat tctaaagaac ctacagtatc agaaatagca aaggaattag
    99121 aattagaagt agaatcagtt gtaatggcac ttgatgccat acaagaccca atatctctgt
    99181 ttgacccagt atatcaagac aatggagatg caatattcgt tatggatcag gttcaagata
    99241 aaaaagatac tgatgaaaat tggttgcaag aaatatcact taaagaagct attaagaaat
    99301 taaatagtag agaaaagttg gtattagatt taaggtttta caaaggaaga actcagatag
    99361 aagttgctga tgaaatcggt atatctcaag cacaagtatc aagaattgaa aaaaatgctt
    99421 taaagaatat gagaaaatat gtataagtaa tccatggact gtaaacatta ttttaattta
    99481 aatgacctat ataggtcatt tttttttact tcgatacata tttattttta aagaaagggt
    99541 gagtacaata tgattagagt ttctgatata atggaaaaag aaattataaa tgtaaaaaat
    99601 ggaaaaagga tggggtttat aatagacata gacatggata ttcacgaagg taaggtagtc
    99661 tcatttacca tttttggaga tggaagtaga agtttttttt caagaggagg ggatgaagaa
    99721 gttatatttt ggaatgatat acttaaaata ggttgtgata caataatagt aaatataggt
    99781 tcagaattaa gcttagatga gtttgagata tagttaaaca aaggaggaat atagatgcaa
    99841 tgtccttatt gtaattataa agaatcaaag gtaatagatt ctagacatac agatttaaaa
    99901 tctatcagaa gaagacgtga gtgtgaatca tgtaaaaaga ggtttactac gtatgagaaa
    99961 atagaaacaa ctcctttaat ggtcattaaa aaggataata gtagagaata ttttgataga
   100021 gaaaagataa aatatggatt gctaaaagca tgtgaaaaaa gacctgtttc tattgacgag
   100081 attgaaagta ttgttgtaca tattgaaaat gaaataaata aatgttttat agaagaaatt
   100141 gaaaccaaaa aaatcggtga gatggtaatg gataaattga aagagttaga tgaggttgcc
   100201 tatgtaagat ttgcttctgt gtatagacaa tttaaagata taaatacttt tgtaaatgaa
   100261 cttaaaagta tactgataga aaaaggagac aaatgatgaa agattatata aatataaaaa
   100321 atgtacaaga tattaaagag tatgttaatt taagtggtaa ttgtgaagaa cttgatgaaa
   100381 aattaaattc tgtaaatcta gttataacat gtaagaatat agatgcaaaa aatagagaag
   100441 atatgttaaa agtatgtaaa gaatgtgatt tgacatttga aaatctaact agtaattctc
   100501 aaatacattc agatatagtt aatatagtca ataaagatac tatagggaaa agaagagatg
   100561 gagatgcact tataacaaac ttggaaaaag taccactttt actgtttaca gcagactgtg
   100621 tacctatatc aattatagat gctaaaaata aagccatagg actggctcat gcaggttgga
   100681 gagggacttt tagtaatata ggtcataaaa caataaaatt aatgtctgaa tgctataaaa
   100741 cagttcctga agatttagta tgtataattg ggccatcaat tggaccatgt tgttatgaag
   100801 tgtctgaaga tttagtagaa aaatttaaca caattcttac aaataatgat gaaaagttct
   100861 atataataaa agagggaagt tactatttag atttatggaa aattaatgag tatatgctta
   100921 gatgtagtgg agttaaaaaa gaaaatataa taaatttaaa tttatgcact agttgtagag
   100981 cagataaatt ccactcttat agaaaacata ataaggcatc agaacgaata ggaacggtac
   101041 tacagataaa atagagagga gctttttaat gaatactaaa gtgcttgtta tagacgatga
   101101 aatgcatata gttgagctat taaaattcaa tttggaggtg tcaaattacg aagttagtta
   101161 ttcatatgat ggatttgacg gatttataaa ggctaaggaa attaaaccag atttaatact
   101221 tttagattgg atgttaccaa atataagtgg aatagaagtt ttgagaaaaa taagaagtga
   101281 taaggactta aaaaatattc ctgttataat gcttactgct aaaaatatgg aaaatgacaa
   101341 ggttgaaggg cttgaaatag gtgctgatga ctatataact aagccattta gtataaaaga
   101401 gttgttagct aggattagtt ctgttttaag gagatataat ttgactagct taggtgaaga
   101461 aaacaatata cttactacag gtaatttaaa actagattta tcaaaacatg aagttaccaa
   101521 aggctctgaa aagatagagt taactcttaa ggaatttgaa ttgttaaaat tattaattca
   101581 aaataaagga aaagtacttt caagaaatta tcttttggat aaaatatggg ggtatgaata
   101641 ctatggagaa actagaacag tagatgttca tataagatat ttaagaaaaa aaatagaaga
   101701 tgaagataaa tcggaaaaat atatagagac tataagaggt gtaggataca aaatagatta
   101761 aaaaggttat attttaggag tgttatcttg tggaagattt aaatagtata tattttttta
   101821 ttgttatgtt gtccatagcc attgttatat tagctgtaag atacacaata acattaagga
   101881 aatatttaaa agagtttata tacgtctcaa aaaaagtaag taataatgaa tatcatgcta
   101941 ggcttaacat atcagccaaa ggagaacttg gcgaattagc tagaaatttt aatcatatga
   102001 taaagaatat ggataaaacc ttagaagaag tacagtataa acatttacag ctaaggtcta
   102061 tattaaagag tatatctcat ggaattttag ctatagatgt aaatggaaat atacttctga
   102121 taaatgatga agcaaaagaa atgataaaga gtgaaccaaa tcaattagtt gaaggtcata
   102181 atattaatgt tgtaataaaa gaagaagaaa tattaaaaca aatacttcaa tttattggtt
   102241 ctaaagataa tgaaactgct aatattacta ctaaagatga tatcgtatat aagataaaag
   102301 tagacccagt ttatcttcag aatactaaca acgtaataat aggttcgata gtaaatatag
   102361 aagatattac taaaaaagtt aaacttgaaa atatgagaag tgattttgtt gcaaatgtaa
   102421 gtcatgaact taaaacacca cttacatcta taagtgggtt tgttgaaaca ctaaagtcaa
   102481 atgaaaatat agatgtgaat actagaaata gattcttaga aataatagaa agcgaatcag
   102541 atagattaaa aaggttgatt gatgatatat tgctgttatc atttgtagaa aataaagata
   102601 ctcttcttat ggaaaatgtt gtagtatatg atgtatttaa agaggtatat gaaataactc
   102661 aacattcagc tagtgaaaaa aatatagaag ttttatataa atgtgatgat aaatcaataa
   102721 gtatactatc caataaagac tatataaagc aagtgttctt aaacctagtt gataatgcta
   102781 taaagtatac ccaagataat gggattgtac aagttgtagt gttaaaagat gaagaaaaaa
   102841 tcgtaattaa agttatagac aatggtgtag ggataccaaa agaagatgta gaacgcatat
   102901 ttgaacgatt ttatagggta gataaagcaa gaagtaggga tgtaggagga acgggtttag
   102961 gattggctat aacaaagcat atagtcaaat cactaaatgg atgtataaaa gtaaaaagtg
   103021 aacttggcat tggtagcgaa tttatagtta caatattaca aaaaaatttc cttaaataga
   103081 agggaatttt ttttgttttt tgcacaaact atataagaga aggagctgag aaaatggaag
   103141 aaagtttatt gaatgcaaat ttatttagac actcttttat agttgcatta gttgtaggaa
   103201 tattatgtag aggattagtt ttaagagtta cagacaaaca atacccatca agaccacaag
   103261 attatttaga acagataatt atatctgggc tttcagcttc gttaggtgct atagcattac
   103321 ctgcacttat agataaggag tttgcagcct tgacattttt tgcagtagca atacaacagt
   103381 ttcaaggtct tgcagaacaa gaaagaataa cactaaagaa tatcgataat gaagaattag
   103441 taccaaaagg agacgcttac gttgaagaaa tagcatctac gtatgaatct agaagttata
   103501 taagtctttt ttctgcactg gtatcttcga tagtttatat cgtttttgca agaaagtatg
   103561 gccttagctt tttttattgc acaatcctag ctattgtgtc aggagctatt gttggtttga
   103621 tttttagaag atttttaaga aggaacagta tagcagatat agcagatata gttcctgcaa
   103681 aaataagttt tgaaggacct attttaatgg taaatggtgt aataataact aacataggtc
   103741 tagaaagtac aagagaaaag tatagaaatg aatgtctggc catagaagtt ataccaagag
   103801 atttaggtgc atttggaata gtaaatgata tagggcaaag acaagctata atacataatc
   103861 tatttattca tatggggata gatagagatg tagatgaggt tgatattgtt gctatatcta
   103921 agaccaattt agaaaaaagt actgtggtta taccatatat gccaatactc aaagatatag
   103981 atgtcatgat agatgtagtg aaaagtacac ctatcataga aacatcaaaa ggaaagcaga
   104041 gtgatttttc tagaaagact ttgtagattt acatgtaaaa tttcaaatta aatattttag
   104101 gagtgattta atggatagag tattttttgt tgctgttatt attggtataa catctagaat
   104161 tataatgttg aatttagacc aaaaacagta tccaacgcaa ccaaatgttt tactatctca
   104221 acttatatta gcattcgtcg cttcttcatt aggagcatta ttggttccag ctcttataaa
   104281 tcgttcttat acatctataa cttttttatc cctagcagca gagcaattta gacaagtgag
   104341 agcaaataga agaaatacat tgcaaaactt agaggaagtt caacttgtaa agagaggagc
   104401 tgcattcata gaagagatag ctagaaccta tgaagtaaga aattatatgt gtatagttac
   104461 atctttttta acaataggat tgtattatgt aatacttgca gaaacgaaaa taagtgaagg
   104521 aatgagtttg gtaataagtt caatttgtgg attagtgtta gcctttatat taaaaaaact
   104581 tcttactaga aaaagtattg gagacatagc tgatgtaatg cctgctgaga ttagttttgt
   104641 agatgagtct atattgaagg taggagattt aaagggaata acaaatatag gtctaaaaag
   104701 tgatagagaa gtatatatta atagaggtgt aggaatacaa ataattccta aaaataaaga
   104761 ttatgctaat gcagggatat tatttgactc aggtcaaaga caggcaatag tatacaatat
   104821 atattcaagg ttaggtttat atggagatta tggagaacct tcattcgtac cattacctag
   104881 aaggaataaa gaggatgaaa gtataatgat tgcttattta ccagtagata agaatgtgga
   104941 aaaagtaatg gaagccgtaa agtcatgccc aatactttca agttctaaag gaaaaaactt
   105001 atcactaaaa aattataaaa taggaaagaa aggaagtgca taagatggga atggatatag
   105061 gtttaaatga atatacccta gctatagtta cacttgataa gaatatgaaa aatggtggag
   105121 gttgtccaat attttatgca aatgatgaga aagacttaca aaataaggca ttgttgatgt
   105181 caaagtgtgt aggtggaatg gttcatgata tgacaaatgg gacgttaatc atagtaaaac
   105241 attaaattta taaaattttt ttaagaacaa ataatattca aatttttaag aaacttaaat
   105301 ttgaatatta tttgttctaa tctattttat agctatttac ttatattgac tattagaatt
   105361 aaaatgtgtt attatgacat gaaatgctta aaaatgctat cataaaatat aataaataaa
   105421 taaattgcat attaaaatat attataataa gcattgatga atacaaaaga gtggtgaaaa
   105481 tattggaagt aaatgtaaaa aaagtaaata atattataag taaggtttat aaagatagta
   105541 ttgcagaaga aataggtata gaggtaggag atttacttat atctgtgaat gaacagccaa
   105601 tacatgatat aatagagtat agattcttac taagtgatga atatttagat gtagaaatac
   105661 aaaaaaaaga tggcgaagtc tacatatacg agatagaaaa agactatgat gaagatttgg
   105721 gtgttgaatt tacaaatccg ataattgacc aagctaaaag ctgtagaaat aagtgtatgt
   105781 tttgttttat agaccaattg ccagaaggta tgagagaaac tctttatttt aaagatgatg
   105841 attcgagatt atctttttta caaggaaatt ttgtcacact tacaaatatg agtgaagaag
   105901 atataaataa tattataaaa tatagaataa gtcctataaa tatatctgtg catacaacta
   105961 atccagagtt aagacaaaaa atgataagca ataaatttgc aggaaaatta tatggtataa
   106021 tgaagagatt ggcagatact catatagaaa tgaactgtca aattgtttta tgtccaggta
   106081 taaatgatgg aaaggaattg gatagaacaa taaaagaact agctcaactt tatccgtatg
   106141 taaacagtgt tgcgatagtt ccagttggga ttacaaagca tagagaaaat ctagtagaat
   106201 taaatatatt taatgataag agcgcaagta aaactataga gcaaatacat caaatacaac
   106261 agaaatatct tgaaaaatta ggtactcgat ttgcattttt atcagatgaa ttttatatac
   106321 tatctaatag tgaattgcct ggatatgaag aatatgaagg ttttttacag tttgaagatg
   106381 gtgtaggtat gatacgtaag ttaaaaacag agattgaaga gtatctaaat atattgcctg
   106441 agaagattct aaaaagagag aagaaagtat caatagctac tggtcattct gcatatgaat
   106501 ttatacagag tatggcagat gctatgatgg acaagtttaa aaatcttcaa gtaaatgtat
   106561 atgaaatcaa aaacaagttt tttggagaga caataactgt atcaggtctt ttaacagcta
   106621 aggatttaaa agaacaactt gaagataaag aattaggaga agctctttat ataacaagaa
   106681 gtatgctaaa agcagatgaa gaaattttct tagataatat tgaattaaat caattagaag
   106741 aacttatgag aataaaaatt atcccttgtt tgaatgaagg aaaagatttt gtggataaaa
   106801 ttttaaaata gaaaacaaaa gatggaagga gaaataaaat gagtataagt aggccagttg
   106861 tagctgtagt tgggagacca aacgttggta agtctacaat atttaataaa tttgcaggaa
   106921 aaaggatatc aatagttgaa aatacacctg gagttacaag agatagaata tttgctgaag
   106981 tagaatggtt agataaatat tttacattag tggatacagg aggaattgaa cctgatagcg
   107041 aagatataat attatctcaa atgagaaatc aagccatgct tgcaatggac atgtcacatg
   107101 taatactttt tatagttgat ggtaaagctg gaataacagc tgctgataaa gaaattgcac
   107161 aattgcttag aaagacaaaa aaaccagtaa tcttagtggt taataagatt gatagtcaaa
   107221 gtcaatttga taatatatac gatttttatg agttgggatt tggaacgcct tttgcagttt
   107281 caggagccaa tagtatggga tttggagatt tattagatga gatagttgaa aattttccag
   107341 caggattaga tacagagtat gaagaagata taataagagt agctataaca ggtaaaccaa
   107401 atgcaggaaa aagttctata ctcaataaga tacttggaga agaaagagta attgtaagtc
   107461 caattgcagg aactactaga gacgcaattg atacttattt tgaaaaaaat ggacagaaat
   107521 ttcttctaat agatacagca gggcttagaa gaaaaagtaa aatatatgag acaatagaaa
   107581 aatacagtgt aataagagct atgagtgctg tagatagagc agatgtagta ttgatagtaa
   107641 tagatgcctt agaaggtgta acagaacaag atacaaaggt tgcaggtata gcacatgatg
   107701 aaggaaaagg atgcatattt gtaatcaata aatgggattt aatagagaaa gataataaga
   107761 ctatgagtaa ttacacaaaa gatataaaag aaaaatttcc atttatgatg tatgctccaa
   107821 tcgtatttgt ttctgcaaaa actaatcaaa gaatgaacaa gatactagat acagttgaat
   107881 atgtgtcaaa tgaacattct aagagaatat caacttcagc actgaatgaa gtaattggag
   107941 aagctgtaat gttaaatcaa ccaccatctg acaaaggtag aagattaaaa atttactatg
   108001 ggactcaaac tgacattaga cctcctaaaa ttacattgtt tataaatgat aaggatttaa
   108061 ctcacttctc atatcaaaga taccttgaga ataagataag agaaaatttt ggatttgaag
   108121 gaacttccat aaagtttgag tatagacaaa aaaataaaaa ataaatatct taaaatataa
   108181 ctaaaggggg gaaattcagt ggagatattt agttatataa ttattgctgt agttgcatat
   108241 cttttaggaa atatatctac ctcatacata gttgcaaagc gaattgctgg ggttgatata
   108301 aggactcaag gttctggtaa tgcaggttcg acaaatgtac ttaggacact tggaaaaaga
   108361 gcaggagcaa tgacattttt aggtgatgtt cttaaaggtg taatggctgt tttaatatca
   108421 gaatttgcag caagattggt tggaatagat actcttttag ctggatatct ggcagttata
   108481 tgtgttgtag caggtcataa ttggccagcg gttttaggat ttagaggagg caaaggtgtt
   108541 gcaacttcac taggggcaat gctagctgta aatccagtaa taacactaat gtgtctagca
   108601 gtatttatat tggtggtagc tataacaaag tatgtttcat tgggttctgt tgtaggaata
   108661 ggatgttcac caatatttat gattatggta aaaaataaag ctggtttaat agtagctctg
   108721 ttcttaactg catcagtaat atataatcac agagcaaata taaagcgatt attaaatggg
   108781 acagagagaa aaattggtca aaagaaagaa tagtaaatat attatactca ggtggtgtta
   108841 aacatggaga aagtatgtgt attaggtact ggaagctggg gaagtgcatt agctttaggg
   108901 cttgcaaaaa aaggtaatga tgtgagcatg tggactcgaa aagaggaaca agctaaaaaa
   108961 ataaacagaa ctaaagaaaa tacagactat ttaccaggag tattatttcc aaataatata
   109021 actataagta ctgatataga aaaaactata aaagattgta aggtaatagt tttagcagtt
   109081 ccatcacagg ctgtaagaag tacttgccaa aaaataaaac catttataaa agaaggacaa
   109141 gttattgtta atgtagctaa aggtcttgaa aaagggactg ggcttagatt atcacaagtc
   109201 tgtgaagaag aattaccaca aaatccatat gtaatactat ctgggccatc tcatgctgaa
   109261 gaagtagcaa gggatattcc tactaccgta gtagttgctt ctaaagattt aaaaatagca
   109321 cagatgatac aagatttgtt tatgagccct aaacttagag tttatacgaa tccagacata
   109381 gttggagtag aacttggagg agcattaaaa aatattatag catttggagc tgggatatgt
   109441 gacggattag ggtatggtga taatgcaaaa gctgccctta tgacaagagg tattagtgaa
   109501 atgagcagac ttggaattgc tatgggagct aatatgtcta cctttgcagg tctttcaggt
   109561 attggagatt taatagttac ttgtacaagt atgcatagta gaaatagaag agctggtata
   109621 ctcataggta aagggatgag tttagaagat actttaaaag aagtaaagat ggttgtagaa
   109681 ggcataactg ctactgaagt agcacatgat gttgctgaaa aattagatat agatatgcct
   109741 ataactaatg ctatatattc tgtaataaag aaaggttcaa atcctaaaga agttggaata
   109801 gaactaatga tgagaagtaa aaagcatgaa atggaagaag tagttttagg tgatgatata
   109861 taaactattg tataataaat aataaattta ttttaggggt gtctcaaaat gaacttttta
   109921 gtttacgagg cactcatttt tatatgaaat taaatatatt tttattattt caacaataaa
   109981 aaaggcttat ctctattttg agacagccat attttaattt tatttagcat gttttattat
   110041 gtattcaata gaagaaatca ttttcatagt taattcatca tcatcagaat tttttcctgt
   110101 gttctttttt aagaataata gattttgata aaaagaaata attatatcat tgattatgtc
   110161 taaatcttta gctggtatat atccagtatc tacaagattg tttaatagta tcttatttct
   110221 ttcctttata ctttctcctg taatcattaa agatatgtca ctatcatgaa tacctaaatc
   110281 ttctggaaac atatcataat attgttttgt tatatagcta agttcatcac tagacaagtt
   110341 aaaaaatacc tggtaaaaag cttgagcatt atcaaaagaa tgcttacaaa ataatttcca
   110401 cataattata tatttcttat attcatcatt gacattttct atacatcttg aaatattcaa
   110461 gttataatct ttaaaatatt ttatagatgc aaatgatatt aaatggtcta aatttttaaa
   110521 gtatttatat agagttgaac tattatagcc agccatatct gcaaccttac gaagtgtaat
   110581 attttctata ccttcgtttt ccataatatt ttgagcagct tctatgaaat acatcaatat
   110641 ccttttcttt tgtaattctt tatttgtcat tttattcacc tacaaataat caatatagtt
   110701 tatataatcg tgattttatc attaaactta ccataaatca agaataatat tacaaaatac
   110761 aatgtatact cgttatattt tagataattt aaaatcacta gaatataaaa aattttttaa
   110821 aataaaatca tgattgacaa aataatcatg attatgataa aataatcaca aataaaatta
   110881 atttacatac aaatacgaca agtactaaaa atattaagaa ttttaaaata atatattcat
   110941 ccttgttgta ttgaataaat tatctgaata ttaagaaaga ggtgcattat gtcaaataat
   111001 atgagactag aaagattgag aaagtacatg aatgaaaaaa gcattgaaat tgctttaata
   111061 tttgaaccag acaatcaatt ttatattagt ggatttaaag caataactta ttctagacca
   111121 attgtaacag tagtaactca agataaaata gaattaatag tacctggatt agaagaatta
   111181 catgctaaag aagttgcaaa agttgataat gtatatgtgt attatgaaat accagaaatg
   111241 aaagaacatg gaatatcaca taaacattac ttagatataa ttttaaataa ataccctaaa
   111301 ggaacaactg ttggaataga aaaagatata gtatctgcaa gcttttcaga gtatataact
   111361 gataagtcat ttgctatcaa agatgttgga agtaaaatat ttgaaatgag atatgtaaaa
   111421 gatgcaaaag aaatagaatt tttgaagata gcaggttacc tatctgatat aggaataaaa
   111481 gggtctttag aaaatgttag agttgggatg agtgaattgg agtttgacgt tgaaggggat
   111541 aatgctttac taaaatatgt ttctgaaaat tatcctgata catatatagg atttgcaaac
   111601 tggacttgtt cagggattga tagaacagca caacctcatt tagattctaa tactagaata
   111661 cttcaaagag gagatatagt aatacatagt agacaggttt ggtatgaaaa ctatagagca
   111721 gaaaatgaaa gaacttttat tataggcaaa ccaacagaaa gacaaaaaga agtgtttaag
   111781 attgcagtag aggctcaaca agctggatta gatacaataa aagctggtat accagcaaga
   111841 atggtggatg aagcagcaag agctgttgtt gcaaagtatg gtctagagtt atattcaaat
   111901 catagaattg gtcatggttt aggtttgtca gaacatgaag agccatattt aagatttgac
   111961 aatgaattaa tattagaaga aggaatggta ttttctatgg aaccagggat atatatacct
   112021 ggagttggag gatttagaca ctcagataca gcaatagtag gtaagaatgg tgctacaatt
   112081 ataactaatt atccaagaag tgttgaagaa cttatacttg atatttaaat caatacatag
   112141 tgtatgagtt tgaataaatt tgaaattgga ggaattacag tgaaaaagaa aattggattt
   112201 tgggatttag tatttatgaa tatatcagca ctatttggga ttagatggat agctaaatct
   112261 actgcctcta gttttggatt gggtcttggt tctataccag tttggttatt gtttgctttt
   112321 atattctttg ttcctgcatc acttatatgt gcagagttgg ctgcaacata tccaaaagat
   112381 ggtggacttg gagaatgggt taaacaagct tatggagaga agtggggatt tatggtgtca
   112441 tggctaaatt ggacatcaaa gatattttgg tattcttctt tcttgacatt tttagctata
   112501 aatattgcat atatgcttgg aaatccagat ttatcaaaca ataagatgtt tattttagca
   112561 ctttcactca taatattttg gctgttgtcc ttagtaagta ctcgtgggat ggcatttgga
   112621 aaatttttta cgaatacagg agcattaggt tctacaattc ctgctatatt acttatagtg
   112681 atggcattta tgtctgtagt tattctcaaa aaagctccat cagcatcgat atatacaatc
   112741 caaaatataa taccaaaaat agatgccaat tcattagttt ctatatctgc tataatattt
   112801 gcattatcag gagctgaaac gactgctaat ttcataaccg aaatggataa tgctaagaaa
   112861 aactttccta aagctatatt aactgtggct gttttaatag gaggaatcta tatcttaggt
   112921 tcagtagcaa taacaatgat acttcctcca gataaaattg cagcatctac agggatacta
   112981 gatgcattag ctaaagttgc acaagattta ggtataggaa gttggtttat aagaatagta
   113041 gcatttggaa taactttatc agtactagga gcattaatac tttatatagc aggtccagta
   113101 aagatgcttt ttggaaatgt aagagaaggt gtatttccta agcaattgac tgttacaaat
   113161 aaatacaata taccatcaaa tgcagtaata gttcaggcta taatagtgag tctacttctt
   113221 gtaggaacaa acttattacc atcagtagat aatatataca atgttcttgt tactatgaca
   113281 gcattaacag cattatttcc atatgtcttg ctttttacat cttatattag acttagaaag
   113341 acaagaccaa atgaagaaag accatattca attgcaaaaa gtgattcagt ttgtatcaat
   113401 attgccagaa tggtacttgt agtaactgta gttggcatac ttttctcaac ggcaccagtt
   113461 atggagactt taaaagataa tataatctat gagattgaaa tgataggagg aggtcttttt
   113521 gttataataa caggtttaat gctatggaag aactatgaaa aaaagatatc taataactct
   113581 ggacgttagt ataaataaaa atataattat tatggtgagc taaaagagtt tacttttagc
   113641 tcataactca ccataaatta ttgatagatt aacctaatat attaataaat attccgaaat
   113701 attgaataaa caatacaata agcaataatt aattatcatg aaaatttaca ggtatttaca
   113761 taaggaggaa gtatgtcagt tgaagtaaat aaaccaagca tttggaaaaa tttcaaagtc
   113821 ttgataatac tctttgtagc aggagcattt atatatgctt taccatattt aaagaattat
   113881 tattatgata cttttgcaca agcatttaat cttacaaata cacaaatggg aagcttagga
   113941 agtatttatg gtctacttgc aatggtatca tacctttttg gtggtttttt agcagaccgt
   114001 atttcagcca gaaaattgct ttcaggttct ttaattatta caggaatatc tggtcttgta
   114061 ttaacacttt atcctccata tattgttgtg tttttaatcc atgccttatg gggaataaca
   114121 acaatactta ctttttggcc agctcttgta aaagctgtta gaatgttagc atctgaaaat
   114181 gaacaaggaa aagcttttgg atttatggaa agtggtcgtg gaattaccaa tgcaattcat
   114241 ctatctctag tattgatact atttgggtat atttcatcaa aagtaagtga taaatctggt
   114301 cttacagcta ttatagtatt atactcggca ataaatgtaa tagttggagt attagtattt
   114361 ttcaaattaa aggataatga aaaagaggta tcagcaaaat ttgataaaga agcctttttt
   114421 gcagtaataa aaatgcctca tacatggata ataacaataa taatgttttg ttcatattca
   114481 gctaatatgt cattttatta ttttacgcct tattcaactg aagcatttgg agctactgtg
   114541 gtgtttgcat ctgcaatctc aatattagct cagtattgta gaccagtagc atcagctgct
   114601 tctggattac ttggagataa agttggttca tcaaagatta tatcttatgg atttatactt
   114661 atgattgcag gtcttttagg tgtaatattt acaccaacaa aatcatctat gatttatata
   114721 ctattgattg gatgtgttgc aatatatgta tctatgtatg caattcaagg tttacactat
   114781 tcgttacttg aagaaggtaa ttattcatta tcaatatcag gaactgctat aggaatagtt
   114841 gctacattag gatatcttcc agaagtactt tgtcctttag cagcagggaa gatattggat
   114901 gcatttccgg gtgtaactgg atataggtac tacttcatag ttcttactat agtagccata
   114961 attggattgg tgtttacatt tatatggatg aacatgacaa aagaaagacg taaagaactt
   115021 attgagatga ataataattc aaatgaaaat actactaaag tcagttaata taatacacta
   115081 ctatttggac cagcaatatt aatttaattt acattgaaat ttacaaattt ctatatgtaa
   115141 attaaattaa tattgctggt ctttattttt ctattttaag aatattaacc cttaactcta
   115201 aatataattt aataagatta gatgtatagg aggaataata tgaataataa catatacgaa
   115261 gatatatcca aaaggactca gggggatata tatattggag tagttggacc tgtaagaaca
   115321 ggaaaatcaa cttttataag aaaatttatg gaaaagttgg ttatacctaa tatagacaat
   115381 gaatttaaaa aggatagaac aagagatgag ataccccaaa gtggttcagg taaaacgata
   115441 atgacagtgg aaccaaaatt tgtaccagca gatggtgtag aaataaaaat aaaagacaca
   115501 gtatctttaa aagtaagaat ggtagattgt gttggataca tagttgaagg agctttaggg
   115561 catgaggaag gtggaaaaca aagattagta tccactcctt ggtcacaaga agctatgaca
   115621 tttgaaaagg cagcagagat aggaactaaa aaagttataa aagaccattc tacaataggg
   115681 atagttgtct tgacagatgg ttcagttact ggaattgaca gaaaaagcta tgtagaacca
   115741 gaagagagag ttatacaaga attgaaaaat cttaaaaaac catttgcagt ggttttaaat
   115801 acgttaagtc caaaaagtga ggaaacgagt atgcttagaa gtgaattgga agaaaagtat
   115861 gaagttccag ttcttccaat gaatgtggtt gaaatggaag aagaagatat agaggaggtt
   115921 atggaagctg tattatatga ttttccactt acagaaataa gaatcaactt accaaaatgg
   115981 gttgaaggtc ttgaaagaaa tcattggata aaaagtagca taataacaac attaaaacaa
   116041 agtattatag atataggtaa aataagggat atagaaggta ttatacaagg attttctgag
   116101 ttagagtttt tggaagatac aggtgttgat aatgtagagc taggagaagg tgttataaat
   116161 attgatttac agacaaagca agatttattc tataatgtcc ttgaagaaaa aagtggattt
   116221 aaaattgaag gagattatca actgcttagt ctgataacta gactatctaa agttaaaaat
   116281 gagtatgata aaatagaaag tgctttaata gatgccaaga taaaaggata tggagtagta
   116341 gctccttcct tagaagaact tagtctggaa gaaccagaaa taatgaaaca aggaaaacaa
   116401 tatggtatca agttaaaagc caatgcacca tcacttcata ttataaaagc agatatatct
   116461 acagaggttt ctccaatagt tggaaatcaa aatcaaggtg aagagatgat aaaatatctt
   116521 atggaagtat ttgaagagca accagcagat ttatgggaat caaatatgtt tggtaaatca
   116581 ctacatgact tagtaaaaga gcaattacaa agtaaattat atactatgcc agaagagata
   116641 agagttaaaa tgcaaaagac tttacaaaaa attgtaaatg aaggaagttc taatattata
   116701 actattttgt tataaaaata tagctactta ttaaagtgaa acgtctgttt gctttagtaa
   116761 gtagcttttt atttataaca attaataaag atacttatat aaattgacaa taattgataa
   116821 ttgtggaaaa attatagtgg gtgaagtata tgtttcgaca ggattgtttc aaagctaaaa
   116881 atttctagga gtgtaagatg aaaaagtatg agatatttat ttttatgagt ataatagttt
   116941 tgtgttttat aagttttcca cataaagttt ttggacaaga ggaaaattgt cttgaagatt
   117001 ttgaaaggtg ggttcaagaa aataaaaata ataaagaaga aatagtttat actctttctt
   117061 gtgatatggt aatagatgaa gagtttagat tttatattcc ttatgacagt aattttacaa
   117121 tagataccaa caaatataaa atattgataa aaaatcatgg gagatttatt attgatgata
   117181 atgaattgaa tataataggt gaaggaggaa aagaaggagt aatacatata gaaaatggtg
   117241 gaagtataag tattggtata aacaacataa ttgctactga tgggacagcc ttatacgttg
   117301 aagaaggagg agacttacat attaaatcat cctacgaatc tgttgaaaaa ataaaggcaa
   117361 atggaaaaga tgcaatagga atttatactg aaaatgatat tagaatacta aataaggata
   117421 ttgaagtgaa tggagaacat gctataggtg tttattctaa aggtgatgtt gagatagaag
   117481 aaacttcaat aaaagcatat agtagcaaca atgaaagtgg gttactaaga gataataata
   117541 agttggcaca atcaattata tcagaaagta agaaagttta tataattgat gattataatg
   117601 agcttatacc ttctatagct gaagattctg gctatgatat tgtgaaatgt tgtcataggg
   117661 gaattggtgt atttagtgat gaaataaaag taagtaaaga tgataaaata gaaaatataa
   117721 aatttcccaa atatatggta ttagaaacat ctagtggtaa tcgacttgat atagatgtag
   117781 aatgggattt tactgattat tatgaaaagt tggaaaaggg agaaaatttt aatataacag
   117841 gaaagtttaa aacagaaatg ttaaataaag aaaaaattat aataaatgat gatgtagttc
   117901 ctattttgaa tgtatctgtt atagagaaaa aaccaataga caatttggaa ctggaatttc
   117961 aaaatacaaa aaatggatat gtagctgttt tattttatga tatgccgtat agtgcaagta
   118021 aggtttttgt tgaatattca agcgatggaa tcaattggat atctgaagaa caggaggata
   118081 ttagagatca agctatactt ttttttgaag attttaagtt gcgttgtttt agagtaaaag
   118141 tggtaggagg attaaaagaa gggtattcaa atatagtttt taaaccagga tttataatgg
   118201 gaggcggaga caatcaagag acgccagatg atgaacggga aaatgatgat attgatggtg
   118261 accgtggagg aggaggtaga gacgacccgg atagagatga ggatgataac aatcaagatg
   118321 ggaataatgg aaatgaaaat aatgatagta ataacaatca aaacaataat aatgaaaata
   118381 ataataatga aaatgataat tctgatgaga aacaaccaaa tactcctaaa gatgatgtag
   118441 aatcaagtac tgatagcaat gaaaataaaa atcctcatga tatacctaaa gacggtaata
   118501 aagaaaataa ttctgataaa gaaaaaggat tttacaataa aaattcaatt acagaaaatg
   118561 atgataatta tgatttaagc tcaaaatcag tatttggtac caatgaacat tcttttcctt
   118621 atagggaaag tatttctaac aaaaatcaag atacaaataa aaataagtca aatataagtg
   118681 attatgatgg aataataaat aataattata aattagaatc taaagaatat aatctaaaaa
   118741 aagatattat ttttttattt tgtatactaa caccaattat gattggttct atattaattg
   118801 taaatcctga tactagaaaa agtataatta aattcataaa attaaaaaaa taatttttag
   118861 aaaggtatta tttagaaaat catgagattg ctaagtaata cctttcatta ttatgaaaac
   118921 tgtatttgtt gttgagaaaa catatataat atatgaattt tctaaatagt ataaaaataa
   118981 tagaatattg aaaaaaaatt gacaaatgaa tgtataggta ttaaaatata ttaaatatat
   119041 acataaaaag tggtttacta acaaataaat gaaatatatg ttaacacata gttcaatttg
   119101 taaaaatata agatttataa aaaaataagg gaggatatta ttatgaaagc aattttaaca
   119161 gtaattggaa aggataaacc aggtatagtg gcaggaatta gtgatgaatt atacaagcaa
   119221 aatatcaaca tagtagatat atcacaaaaa atattacagg attattttac tatgattatg
   119281 atagttgatt tagagaaatc attaaattct tttgagaaaa cggttgagga cttggttgaa
   119341 agaggtaaga aattatcagt ggatgtaaaa attcagcgag aagaaatatt taattcaatg
   119401 cataatctgt agtagacagt aaggggaggt gataatagtg aatgtaggaa atatacttga
   119461 aacaataaag atgatagatg aagaacagtt ggatataaga actataacta tggggatttc
   119521 tttattagat tgtatagacc ctgatggaga taaagctaga gaaaaaatat acaataagat
   119581 tatgagttct gctaaagatt tggtgaaggt tggaaaagat atagagcgtg aatttggtat
   119641 cccaattgta aataaaaggg tatcagtaac tccaatttcc attattgcag gtgcaacaga
   119701 tgaaaatgac tatgtaaagt ttgcacagac attggataag gctgcagaag atttaggtat
   119761 agattttatt ggtggattct cagcattagt tcaaaaggga tatacaaaag gtgataagat
   119821 actaattaag tcaattccaa aagctctagc aagtactaat aaagtatgtg cttcagtaaa
   119881 tgtaggttct acaagatgtg gtataaatat ggatgcagtg aaagaaatgg gagaaatcat
   119941 aaaagaaact gcagaattaa caaaagatgc aaaaggtttt ggttgtgcaa aattagtagt
   120001 attttgtaat gcagtagaag ataatccatt tatggcagga gctttccatg gtgttggtga
   120061 ggctgataga attataaatg ttggtgtaag tggccctggt gttgttaaga gagctttaga
   120121 gaaagtaaag ggagagcctt ttgatgttgt ttcagaaaca gttaaaaaga ctgcatttaa
   120181 aattacaaga gttggacaac ttgtggcaaa ggaagcttct agtagattgg atgttccatt
   120241 tgggataata gacttatcac tagctcctac acctgctatt ggtgattcgg tagcaaatat
   120301 attagaggaa atgggtcttg aagttgttgg tactcatggg actacagcag cattagcact
   120361 tttaaatgat gctgttaaaa aaggtggagt aatggcatgt tcacatgttg gaggacttag
   120421 tggagcgttt ataccagtat ctgaagatgc aggtatgatt gatgctgtta ttaaaggtgc
   120481 tttaagtata gataagcttg aagcaatgac tgctatttgt tctgtaggtc ttgatatgat
   120541 agctgtgcca ggaaatacta cagcaggtac gcttggagcc atgatagctg atgaggcagc
   120601 aataggtatg attaataata agactacagc cgttagaatt ataccagccc caggatgtga
   120661 tgtaggtgat atggtagaat ttggaggact tttaggacga gcaccagtaa tgcctataaa
   120721 tacaaattca tctgaattat ttactcaaag aggaggaagg attccagctc ctatacattc
   120781 atttaaaaat tagttatatt acatataata atatttaatt aaatatttag atggttaaat
   120841 taaagaagtc tatgcattta aatattatat aagataatga actttgttaa gataataaac
   120901 tttaaaaatc agttgttaag attatggcaa aaataaagcg atacatggac atttaaaaat
   120961 tcatgtatcg cttttatttt aataatatct aattaatata aaatttttta tgtatctaaa
   121021 aagttatgct atctaactag taataaacta tactaatttt tattcctaat atatcaacca
   121081 aataaaaaac taaacaaaaa tctaaataaa aataataaat aaaaatttat ctaaatattc
   121141 aacacacata ataatcataa acataaataa aaaataaaac gtttgcaatt aataaaattg
   121201 cctacaatat aaaactctac tacttgagat ataaatttat aaatcttatt gttttttaag
   121261 caatttcttc ttctacatca gtaccacgta tttcaccagc agtaagaact ttaaaggtac
   121321 tgaatatttt tgaacctaaa aatcctgcaa ttacattgaa taaagcacat aaaccagctg
   121381 ttataagaac ttgttgaact ggattataag caaacattat agcaaaacca gccacaggtg
   121441 tagctgtacc tggtgtcatg tttacaagac ccatcaaggc tacaatgata ccagaaaagg
   121501 ctcctccaaa gaaattagta gaatatatag gtattggatt agctgaaatt atgtctgatt
   121561 gggttaatgg ttcaatagca acagatataa tatctttttt agaacctaat ttcaattttg
   121621 caaatagtgt tgagttcata aaagctgaac caaaagcacc taatgctcct attgccatag
   121681 gaagaccagt aagaccaatt atagatgtaa gagccattga actaagtgga gacgttccaa
   121741 ctactgttat tagacctcct agtataattc ccattattat agggctaatg ttggtagctt
   121801 ctatcaaaac ttgtccaatt tgtagtagtg tactattaac taatgggtct acatttgatg
   121861 ctataagacg agatagcggt gcacatagaa gaattacaac cactaaatcc actccaggtg
   121921 gcactttttt ctctataaac ttaattaaga aagaaacaaa gtatccagca acgaatccag
   121981 ataatataga aaaatctaaa caagataatc ctattaaaac tgcataaact ggagatacac
   122041 ctaaggctaa aggcacaagt atagcagcag caactccacc aagactacca ttagcagctc
   122101 caacacttct aaaaaattcc atacctaaaa catcaccaag taaagaaagg ttgaatgcct
   122161 ctactaagaa agtagcacat gcagcgtttg ctagagctcc catagccttc ataccatatg
   122221 gggccttgta actaaaaatt gtgaaacaac atagaaccaa taaaagtaaa cctgttccta
   122281 ataaaatttc tgacataaga aaatcctcct tgtatttttt ttaggacata aacttttgtt
   122341 atgataacgt tatcctaatt cataaatgat tacctaaata aatgtcaaca taacaaaagt
   122401 tattttaaat cttaattggt tttatattgc atctaaaaat aaaaataact ataaaaacat
   122461 ataaagtaag cttaacgtat aaaatgtaaa ttaaacttat actttataat acaattatat
   122521 ataaataatg cagtcttgta taggaagatt gataaaaaat aaatttaata tggaaaaaat
   122581 aattattaga acgtaaaata ttagcttata aaaaaggaat aaacattttt tagaagaagt
   122641 acttataatg aaagtaattt tataggggga gagtctttga aaaaggtaaa atataccaga
   122701 ttagtgatac ttggtattct tatatattta gtatttaata tattagtaat gataattagc
   122761 aaaaatgtag atacgttggt gttaaaaaat gaaaaagttg atgcaaagat tcatgtaaaa
   122821 ggaactatag aaaaagatgc caatgaccag agtaattatc tatctacata catcagtaaa
   122881 gaagattcta ataactttga aaaaaatcaa aatgtaataa ttgaatatca tgagcaaaaa
   122941 ttagatgcaa aagtttacaa aatatatgaa aataataata aaataatggt aaaattaaaa
   123001 ataagtaacc aaattgttgc aaatcaggat acaagtgtcg aagaatttga tataatatat
   123061 aatcagatgg aatgtttaaa aatacctaaa acttctataa aaacaaaaga caataaacgt
   123121 ggtgtatatg taattgatga acaatcacaa agtgtaaaat ttgtcatatt agaaggaata
   123181 acttatgaga atgaatcttc tgtatttgtg gattattata aaaatgacat aaatggagtt
   123241 acaagtgtta atctatatga caaaataatt ttaagaccta acagtataaa cacaaatata
   123301 aggataaagt aggtgataat aatgggttct attaaagaca atttgtctga gataagaaac
   123361 aaaattgata aagctgcaaa aaaagtagat agagacagca aagagattac ccttttggca
   123421 gtcacaaaga ctgttgatgt agacttagtt aatgaggcaa tgagggaagg aatcacttct
   123481 gttggtgaaa ataaacccca agaattagct agaaagtatg atattatagg tgataaatta
   123541 aactggcacc tgataggaac tttgcaaacc aataaagtta agtacataat cgataaagtt
   123601 tgtatgatac actctttaga tagaattgca ttatgcgaag aaattcaaaa acgtgcagaa
   123661 caaataaaca gagaaataga ttgcttagta caagtgaata tatctaaaga agagtctaag
   123721 catggacttg atagagaaga agttgtagac tttgtcaaag aagtgtcaga aaaatataaa
   123781 aatataaaaa taaagggtct aatgacaatg gcacctttta tagaagatga agatgagata
   123841 agaggtgtat ttaaaggtct taaagattta tcaaaacaaa taaaagaact aaacatccct
   123901 aatgtaggga tggatgtttt gtcaatggga atgagtcacg attatgaaat tgctatagag
   123961 gaaggggcta ccattgttag agttggaaca tcaatctttg gagaaagaaa ctacaataaa
   124021 aaataattac tcaaatttga ataaatttta aggaggaagt taataatgtc aaatggaata
   124081 atatctaaat ttaagaattg gatagttgat gaagaagaag attacattga agatgaatat
   124141 gaaagtggca tggacgatat agttcaagaa gaagaaatga acagtggctt tagtactgca
   124201 aaagcaaata aaattgtaaa tttacataca actagccaaa tgaaagttgt aatagttgaa
   124261 cctaaggttt atgatgaagc tgcaactata gcagaccatc taaaacaaag aagggcagtt
   124321 atagtgaatt tggaaggatt gactaattct gaagttcgta aatctatatt taactttatg
   124381 aatggggcag tttatgtctt agatggaagt atacaaaagg tttctaaatc aatatttata
   124441 ctagctccta ataatgtgga tatagatgca aatatgaaaa aagaattaga aagtaaagca
   124501 ttcttccctt ggcaaaataa atagtaaaaa ggcggttaat atatgggaac aataagaatt
   124561 gcactttatt atcttttaga tataatagca tggatgatag ttataaaaag cttgatgact
   124621 tggtttccaa atggaagaca aagtaaaatt tttgatattt tggaaaatct tacagagcca
   124681 atagaagggc caataaggtc tataatgtat aaatatacaa atggacctat agatttttct
   124741 cctttaattg caatagtatt attgatgttc ctaagacaat tagtattggt tatattctaa
   124801 ggtgatagga tggataaatt aaaactaaca aatcacataa aggatataga cttgaaaaat
   124861 aaaatgttta aagtgataga caaggctaat agttgtataa aaaactatga tgttaagtct
   124921 acggagtttc ttaatccata tgaagtgaaa aatgctgtag caatattaaa ttctacaaat
   124981 gaaataaaat acagtgtgga tggaggttat gaacaagctg aaagaagcac tgtatttatt
   125041 tatccatttt acatggaata tgaagatata gaagatactc ttagattttt acaaatagaa
   125101 gggaatttca aatttaaaaa tatatctcat aaagattact tagggtcaat actgggtcta
   125161 ggtataaaaa gagaaaaaat tggagatata attatacatg atagcttttg tcaagtagtt
   125221 gtaagtagtg atatttgcga ttttataatt gtaaacttag aaaaggtttc aagaaataat
   125281 gtaattgtaa aggaaatttc cagagaaaat attgttaata gttcttcaaa atataaagaa
   125341 gtatcattta cagtatcttc agataggtta gattgtgtga taagtggtat ttacaatata
   125401 tctagacaag atagtgcaaa atacataaat ggagaaaaag tccatgtcaa ttatgaaaag
   125461 ataacttcaa cttctaaaat agtaaaaaat gatgatttga tttctattag aggcaaagga
   125521 agagctaagg taacacaaat aggagatatt actaaaaaag gaaaaataaa agttcaagca
   125581 agattaattg tataaggact aatttaaatt atagctggga ggaaatgtaa tgctaactcc
   125641 aattgagata gaaaataaag agtttaaaaa aggtttaaga gggtatagag atgaagaggt
   125701 tgatgaattt ttagatatca tcaaggaaga ttatgaatct ctatgtaggg aaaatacagc
   125761 tcttaaagaa aaattagggt tatatcaaga ccaagtaaac aagtatgaaa atattgaaga
   125821 aacactaaaa gctactttaa taactgctca aagtgcagca gaagacactt gtagcgcggc
   125881 taataaaaag gctaagataa ttgttgaaga agctgattta aaagctagac aaattataga
   125941 acaagctaat aaccgtgtta tagagataag aaaagagtat gatgctttag tcaaagaatt
   126001 taagatattt agaaataaat ttaaatcact gttagaagat gaaataagaa gtgttgatga
   126061 gattttttat gatgtagatg aaaaatgtgt aaacgcattt gaaggaacaa cagtgtataa
   126121 ttatagtaat gatgaagctg ctacaacttt agaataagct tgtaatttgt ttatttttta
   126181 tgtagaataa ttgtgtgagt ttgagaaatc tataacaaag attaaaaata ggataaaaat
   126241 gtaagaaaaa tagaaattat tatatttttc aaaaatatat tgactattca aattatttgt
   126301 tatagaattt catataaaca cagataaatt tttaaaagcg ttgatagaga catttatctt
   126361 tagataattt tacagagaat tgggtatgct gagaaccaat gaatttataa agataaaaat
   126421 cactcttgag ctgcaggctg aacgattaaa ttctactaag ctgtgccggt tagtaccgtt
   126481 atactattag agtgttatat gtagctagta tttagtttac atatagaatt agggtggtaa
   126541 cgcgatttat aactcgtccc tttggtttaa agggggcgag ttttttagta tttaaattta
   126601 tgtgaaagaa taaaaaattt tggaattata atatttagta ggaaagggtg atttacatgg
   126661 cgaaatttaa gccattggta gacagttctg taaaacaagc agaagcacaa gtttttgatt
   126721 attggaaaga cataaatata cttgaaaaaa ctttagaaaa agggaaagat gacccaagtt
   126781 tcgtattcta tgaaggacct ccaacagcta atggaaatcc tggagtacac catgttttat
   126841 ctagaacgct taaagattca gtttgtagat ataaaacaat gtctggttat caagtaaaga
   126901 gaaaagctgg ttgggataca catggattac cagttgaaat acaagtagag aaagagttag
   126961 gacttactag taagcaacaa atagaagaat atggaatagc tgaatttaac caaaaatgta
   127021 gagagtcagt tttctcattt gaaaaacaat ggagaataat gactgagaga atggcttatg
   127081 aagttgattt agataatcca tatataactt tagataataa ctatatagag tctgtatggt
   127141 ggatactaaa caaattcaac aaagaaggat atatatatga agggcataaa atacttcctt
   127201 attgcccaag atgtggaact ggtcttgcta gtcatgaggt tgcacaagga tataaagaag
   127261 taaaaactaa tacagttata gctaaattta agaaaaaaga tgctgatgaa tacttcttag
   127321 catggacaac aactccatgg actttaccat caaatgtttc attaacagta aatgctgatg
   127381 tagattattt aaaagttaaa aaaggtgatg aggtatatta tgtatctaaa cctttagctg
   127441 ataaagtact aggtgaagat tatgaagttt tagaagaaat gaaaggtaaa gacctagaag
   127501 gtttagaata tgaacaatta atgccttttg tagaagttga caaaaaagca ttttttatta
   127561 cagtaggaga ttatgtaact actgaagatg gaactggtat agttcataca gcaccagcat
   127621 ttggtgaaga tgactacaac acaggtaaaa gatatgattt accagttatt caaccagtag
   127681 atgaaactgg taaatttaca tctactcctt gggaaggtag atttgtaatg gaagatgggc
   127741 tagatgttga aataataaaa tggctagctt ctgaaaataa attatatagc aaagaaaaag
   127801 tagttcataa ttatccacac tgctggagat gtcaaactcc attagtatac tatgcaaaac
   127861 caagctggta tatagagatg actaaactta aagataaatt aatagaaaat aataatggtg
   127921 taaaatggtt cccaagtttt gtaggagaga agagatttgg aaactggtta gaaaatctaa
   127981 atgactgggc aatatcaaga actagatatt ggggaactcc acttcctata tggagatgtg
   128041 aatgtggtca tacagattca gttggttcaa gagctgaatt agctgaaaaa gctatagaag
   128101 atgtaaatcc agaaacagtt gaacttcata gaccatatgt agatgatata cacttaaaat
   128161 gtgaaaaatg tggaaaacca atgacaagag taacagaggt tatagactgt tggtttgata
   128221 gtggggctat gccatttgct caacaccact atccatttga aaataaggaa aactttgacc
   128281 aattgttccc agctgactat atatcagaag gtatagacca gactagaggt tggttctact
   128341 cattacttgc agtatcaaca tttgtattag gaaaagcacc atataagagt gttttagttc
   128401 cagaccttgt acttgataaa gatggtaaga aaatgagtaa atcaagagga aatacagtaa
   128461 atccaatgga attatttgac caatatggag cagatgcatt aagatggtat ttactatatg
   128521 tttctccacc atggactcca actaaatttg atatggatgg attaaaagaa atccaaagta
   128581 aatttatagg tactatgaag aatgtatata acttctttac tttatatgca aacacagatg
   128641 atataaatcc aactgaattt tttgtagaat ataaagatag accagaactt gatagatgga
   128701 tattatctaa gttcaataat ttaatgaaag atgtagaaga aaatttagct atatttgagt
   128761 taaataaaac tttaagaatg atacaagatt ttataaatga tgatttatct aactggtata
   128821 taagacgttc aagaagaaga ttttgggcaa ctgaattaac agaagataaa aaagcagtgt
   128881 acaatacaac ttatgaattg ttacatggtc tttgtagagc tatagctcca tttgcaccat
   128941 atatgtcaga agaaatgtat agaaacttaa caggagaagt atctgttcat ttagctgaat
   129001 atcctaaatg taatgaagag ttagtagata ctaaattaga agaaaaaatg gatttagcta
   129061 aaaacttagt tactttaggt agagcttcaa gagaagtaga aagaataaaa gttcgtcaac
   129121 cattacaaaa agtgttagta gatggtaagt ttgaagatac aataagtgat gtagttgatt
   129181 taataaaaga agagcttaat gttaaagagg taatatttgc taaagattta gatgagtata
   129241 tgaattttag tttaaaacca aactttaagg aagcagggcc attattaggt tctaagatga
   129301 atttattcgt tggagcatta agtaaattaa atgctcatga aactgctaat aaacttgaaa
   129361 aaggtgagac tttaacagtt gatttagatg gtgaagcttt tgaatttaat aaggatttag
   129421 tattaatagg tataactgct aaggaaggat ttaatgtatc tgttgaaaac aatctttttg
   129481 ttatactaga tactaagcta actgaagagt tgattaatga aggttatgct agagaattta
   129541 tatctaaagt acagcaatta agaaaatcta ataactttga agtattagat aatatagtaa
   129601 tagaatactg tggtgatgat gaaattgcta aagctgttga ccattttaat gaatatatca
   129661 agtctgaaac tttagcttta gaaataaaca gagtagatga taaatcttta gaggaacaaa
   129721 acttaaatga ccatatgaca ggtataaaag ttataaaaaa atagttagtt aatcaaaatc
   129781 cctatagaaa ttagtttata gatttctata gggatttttt atgtaatata attatctatt
   129841 caataaaatg ttttaatatt ccaaaattta caataaataa atctgtataa atcatataaa
   129901 ttatttatgt catgtgatag aatattaatg aataaacgtt taagtgagga tgaataatat
   129961 ttttaggaaa ctacttctga taaatttatt tgtaaattta tcgtgtgtta tgcagggtgg
   130021 tttatttatt atgcaaaggg gtgcgttttg atgtatggtg aaagtaaaaa gctaagtatt
   130081 gataataata tatttttaca atattgtctg attatattgg gtatgattct atcaacctta
   130141 ggaataaatt tatatctagc accagccaag ttattaagtg gaggagtagc tggaatttgt
   130201 gttatattat acaaattatt tggtataaat cagggaatat caagtttctt gatgaacata
   130261 ccaatattta ttatagctag aaagtatttt gataataaat ttttgctaat aagttttgtc
   130321 aatatgttac tattttctct agcactgggg ctaacacaag atattgctaa atatttccca
   130381 atagatgata ctatgctaca gtgtatttat ggtggtgcat taactggtat aggtatgggg
   130441 ttaactttta aagcaagagc tactgctgga ggacttgata taatagcagc cataatgaaa
   130501 agaaagtatg atatacctat gaaaaataca tttttattta ttaatttttt tgtagtatgt
   130561 gcaggagctt ttttatttgg agctaaattg gtgatgtaca ctttaattac aatgtatata
   130621 atttctttta ctatggatat aggaaaagat tgctttgata gaaaaaaatc tatactttta
   130681 atatcaaata aatatgatga aatatccaaa gtgataatga ataaaatggg tagaggagtc
   130741 acttttttgg aggcagaagg tgcatataca caaaataaga aaaagatgat ttattgcata
   130801 atatctgcaa atgaaatagg taaatttaaa gatttaattt atgatatgga tagtgaagct
   130861 tttatttctg tcaataacgt tgaagaagta aaaggtggag gttttaagga taaatttctt
   130921 taagattata tatgttaatt atatatgtta aaaaatgaca tatgtattga gattatacct
   130981 agttagatgt ataccatagt taatggggta catgaagtac tcaataatat actaggaggt
   131041 ataagaatta tgttaagaga gagatttgta gtaagaaatc atcataatcc acatacaagt
   131101 gggtgttgtg ggactggagg agaaggatgc tgtggaggag ataaccatga acattcacat
   131161 caacattctc acagtcatgg gcatgaacat gagcatgtaa atggtgtagg tcacgaccat
   131221 aatcatgatg atggtttttc aggtactcat gagcatagcc atgagcatac acatacacat
   131281 ggtcatgaac attcagatgg gcatacacat gaacataaat aaaaacattt taaatttata
   131341 agagattttt attttgtgat tccatgaagg tttaaataaa aattgaatgt tttaagtaaa
   131401 aattattgag aataaataag aaagtgaact gttaaagtag ataacttaaa tagttcactt
   131461 ttattaatta tctaagtatt aagtttaaat ccaatgatta actaaaaaag aagctatatt
   131521 ttcataaggt attttttctg tttttaataa actttggaca actaaaccat caactattga
   131581 catgagtaaa aaagaaaatg attcagcatc ctgttcattt tcgctactat tatatttttc
   131641 tagtgattca acaatttcag atttaagagt agttctccat tcaatatatt ttaatcttat
   131701 tctttccttt aaatcattat ttgaagtaat tgcctcacaa ataagataca tatgtaccct
   131761 accacgactt tctatagtag atattttctc taagattaag ttaaccattt ctatttgaga
   131821 attttttgat ttgacatttt ttacacagtt tagtatagca ctagttataa cttctaaatg
   131881 agtatccgca atatcatata taatatcatc tttagatgcg tagtgataat acaaagtacc
   131941 tttacttata tttacatctt ttgcaatatc agcaaggcta gtattttgaa ctccattttt
   132001 ggctattaaa ttggttgcag atgataaaat agcctgtttc atattatttg ttttcataaa
   132061 acctaccttt ccatgtatat ttataacaaa ttaataacat tataagctta tggatattta
   132121 ttgtcaataa agcgagaatc tatatctatt gacttttttt cattcaatat ataaaataat
   132181 aattaaatcg gtcgtacgac cgactgaata aataaaataa tatcttatag tgaggttaat
   132241 atggataaaa taaaattgat agtggacagt gcatgtgact tacctgatga tataatagaa
   132301 aaatacaaca tagaagttgt tggtttaaat gtatcttttg gagaagaaag ttatatatct
   132361 ggaaaggaaa tagataatga aactttctat agaaaaatga gtgagagtaa agtactacca
   132421 aaaacatcat gtccttctcc tgataaattt ttagaagcat accattgtca ggaagaaagt
   132481 gtattagtga ttaatataag ttcaggatta tctgggactt ataatagtgc acttttagca
   132541 aaagatatgt ttgaaaagga aggaaataac aagaaagttg aggttataga ttcacttagt
   132601 gggtcaattg gaataggaca attagtgtta aaagcagcaa tattaataaa agaaggaaaa
   132661 gaaattgaag agattgtttc aataataaat aaatataaag ataatcctcc attttttgga
   132721 acacttgaaa ctttagaaaa tgctattaag ggtggtagaa taaatccaat agcaggaaaa
   132781 attataaata ccttaaattt taaggcgata atacaagttg cagatggcgt tgtaactcct
   132841 atagataagg caagaggtga aggaaacagt ttgaaaaagc tgattacttt agtagagtca
   132901 aaaattaaag ataaagaaga aaaggtttta tttataggac atgctaattg cccagaaaag
   132961 gctcaaaagg taagggaagt tatggaaaag gatgtaaaat acaaagatgt tgtaatctgt
   133021 gaagttggtt cagtgatggg gacatatact tcaaagggtg caatcttaat aactgcaata
   133081 taaatactgc aatataaata atggattgaa ataaatcata gattgagaaa ttaaagccat
   133141 ataaaaatgt ttaaaacatt tttatatggc tttttaattt tatcaaattg attttagaat
   133201 taaccaacta cacatatttc tttgtcatgt ttgtttaata tctcacaacc atcttcagta
   133261 acaagtacta aatcttctat tctaactccg aactcacctg gtaaatatat acctggctct
   133321 atagagaata tcattcctgg ttgtacagta tctgtattta tagaagatac atctccttta
   133381 tcatgaactt caagtcctat agagtgacca gttctatgag tgaagtactg tccatatccc
   133441 ttttcagtta tgtagtcacg agcagcagca tctatatcac agaatctaac acctggtttt
   133501 acaatagctt cagctctctt attagcttca agaactattt caaatatttc tctacctttt
   133561 tcaggtattt ctttatagaa tacagttctt gtcatatcag cacaatagtt atcttttata
   133621 caacctatat ctaagattat tgcatctcct ggtttcacaa gtgcatttcc aggttcacca
   133681 tgtggattag ctccattagg tccaaaacct ataatagggt caaatgaaaa tccatcagta
   133741 cctaggtctt catatatttt agataattta ccaacaagtt gtttttctgt aagttcacca
   133801 gaaacagtag ctttaagttg ttccatagcc ttgtcattta attttgaagc tattctcatt
   133861 aactcttttt cttcttcatc tttacacatt cttaaagtat ctattatata agatgaattt
   133921 acaaacttac ttcctccacc taattctatt aagtttaata agaatctagc tggccagttt
   133981 ttatcaacac ccatagtagc atttttgtct atatgttcag ttattatttc tactggattt
   134041 tgagtatcat tgaaccaaac catttcaaca cctaaatctt ctgaaactgg gaataattca
   134101 tttataaata atttattatt gccatttaaa tttaagtata atgctaatag tctttcgcct
   134161 ggatggatta gtactcctgt aagataaaat atagaagtag ggtcacttac taacatttga
   134221 gaaatatcat cttttttcat ttgttcaagt acagcattta atctttgagc tttcataata
   134281 atttcctcct aatcaaaatt caatacaaat caattttagc attattaaac aaaaaagagt
   134341 atacttttct tctagaaata taaaaaaaat ttgttatata ttttttatat taagtttaat
   134401 ctctactttt gcattaaata cggaagtaaa atgacttttt attgtgaaaa ttctataaaa
   134461 gtaataaatt aagtctataa agttat
//
LOCUS       DAEPIN010000002       127618 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.002, whole genome shotgun sequence.
ACCESSION   DAEPIN010000002 DAEPIN010000000
VERSION     DAEPIN010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 127618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 127618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..127618
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.002"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            335..1204
                     /locus_tag="KR250_000114"
     CDS             335..1204
                     /locus_tag="KR250_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBH0066087.1"
                     /translation="MDYKTIILNRKSVREYKNTDIKEEYLSEIKKYSESCKKLVPEID
                     VDVRIMNRSEVFNNLDKVAGYKGNMIDSPSYIIILSDIKDNYIENSGYIGESIIFKVT
                     ELGIGSCWVTFEDGKKVKEKLGINSDKEVTGIIAIGYGENVSNAKVLNATKTGQNYSK
                     SDMQVVTDKGSTRLGVEDVVYMNEWGNVASIEILEERALLDAFHYARLAPSTLNRQPW
                     RFIVDGGTVVLAVRKDGHTNLYEEKIDIGIVMLYFATIISATMFDLKWNLGTPDKDYK
                     VPEDYKIVGYCNI"
     gene            1430..2296
                     /locus_tag="KR250_000115"
     CDS             1430..2296
                     /locus_tag="KR250_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBH0066088.1"
                     /translation="MEWINKLNQAITYIEENLESGVDYAEAAKIACCSTFHFQRMFSY
                     IAEVPLSEYIRRRRMTKAAFELQNSNIKILELSEKYGYDSPTSFNRAFQNIHNISPSA
                     ARAKGVVLKAYPKMTLSIYVKGNIEMQYRIVEKKGFRIVGIKESMNMIVEECFEKVPK
                     LWAKCIKNGTIDKLSELINNEPCGLLGVSVCTNSKGLDYYIAAPTDKPVLEDTHEYLI
                     PSGTWAVFECVSPMPNALAIQELQKRIITEWLPSSGYVYANLPDIELYPNGDINAPDY
                     TTEVWIPIQKPS"
     gene            2488..2685
                     /locus_tag="KR250_000116"
     CDS             2488..2685
                     /locus_tag="KR250_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004631111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0066089.1"
                     /translation="MKNRLEEIRKERGIKQEELASILQVSRQTIGSLENGRYNPSIIL
                     AFKIARYFDMSIEEIFIYEEE"
     gene            2688..3131
                     /locus_tag="KR250_000117"
     CDS             2688..3131
                     /locus_tag="KR250_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0066090.1"
                     /translation="MKKNMLYVGIIYFICGILLVLLATFTEFSFEAFIWGLSGAALGP
                     GVCMILQYMYWSKPERAVDYEEKIKNQRIEMNDERRIMLRDKAGRITNQIMCYVLVIL
                     IFIVSILSVFSVMVISKWVLVVLGLLILFQFICSVVVYNKLDKRL"
     gene            complement(3318..3405)
                     /locus_tag="KR250_000118"
     tRNA            complement(3318..3405)
                     /locus_tag="KR250_000118"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3369..3371),aa:Leu,seq:caa)
     gene            3557..6205
                     /gene="polA"
                     /locus_tag="KR250_000119"
     CDS             3557..6205
                     /gene="polA"
                     /locus_tag="KR250_000119"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HBH0066091.1"
                     /translation="MEKKLIIIDGNSIINRAFYALPEMNNKEGLKTNAIYGFTTMLFK
                     MIDIYKPTHISVAFDRKAPTFRHLEYKDYKAGRKGMPDELAEQLQPLKDLLDKFRINR
                     LEIDGYEADDIIGTVSKKAEENGYKVYIVTGDKDAIQLASDNTTTLITKKGVGEVEEY
                     NFNSVIEKYEMTPTQFIDLKGLMGDKSDNIPGVPGIGEKTGIKLIKEFSSIENLIENT
                     DKLKGSVKKKIEENKEIAIQSKRLATIIRDVPIEVDLDSMIFGDYDKDELLDKFRYFG
                     FTSLLSRLVDLVDSDDTEHVEEKIEILKLKDIEKFIDEVNKKGQVILKTVRGQGNILE
                     KNIIYIFISVDGEKIYYVDSNEVSKLDSIFSNNEIKKYGYNLKDDYISLKPYNINLEN
                     MYFDITIAEYLIDSTSSTSYDCSAIAMKYLSKKVKSKEELLGKGVKSKNYEDLEFEDL
                     AEYMGQIICTVKETVPMMERSLMDMSMDGLFYHVEMPLVEVLGHMEYEGIKVDKSILD
                     ELSVEFKEIIATLEKEIYELSGEPFNINSPKQLGVILFEKLELPIIKKTKTGYSTNAD
                     VLEKLRDKHPIIDKITEYRQIVKLNSTYVEGLLNIINPESNRIHSSFNQTITTTGRIS
                     STEPNLQNIPIKMEMGRKIRKVFIADDNCKLVDADYSQVELRVLAHMSQDENMIEAFK
                     HHEDIHTKTASQVFNVPMEDVTSELRGAAKAVNFGIIYGKSDFGLADDLNIPVPKAKE
                     YIESYFAKYHKIKEFMDTTVEKASEDGYAVTILNRRRYIPEIKSSNFMERNRGKRFAM
                     NAPIQGSAADIIKIAMINVHKKLEENNLKSKLILQVHDELIVEAIDDEIDIVKKIVKE
                     EMENAVNMDVHLDVDLNVGSSWYETK"
     gene            6214..6816
                     /locus_tag="KR250_000120"
     CDS             6214..6816
                     /locus_tag="KR250_000120"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HBH0066092.1"
                     /translation="MLILGLTGGIGCGKSSLSNIFRNLNIPIVDADIISRKIFEDKLL
                     LEKVFVHFGQSIKNDDGTLNRKALGKIVFSDEEKLKELNNLTHPRIREKIISEIEKLR
                     KKGENIVVLDAAILVESGFLDMVDKLLVVTCKQEVQISRIQKRDNCSEQEALSRINSQ
                     MSQEEKSKYGDYIIDNSGTITELESKAHKFIEYMKENWRE"
     gene            6809..7363
                     /locus_tag="KR250_000121"
     CDS             6809..7363
                     /locus_tag="KR250_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HBH0066093.1"
                     /translation="MNSKKVLILSIFIILFGALLMESKVIHKFLYPKKYSEYVEKYSK
                     EFNLDENIVYSVIKAESKFNSSAVSKKEAKGLMQILDITRDWGAEELNLKNVDIFDPE
                     TNIRLGCWYLSKLYKEFGKLDLVIAAYNGGSGNVKKWLENNEYSKDGENLHDIPFKQT
                     SKYVEKVKNNYEHYNKIYGKKGKN"
     gene            7363..9078
                     /locus_tag="KR250_000122"
     CDS             7363..9078
                     /locus_tag="KR250_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBH0066094.1"
                     /translation="MKRIKVLTVVLAITLMVAGCSNTKTKQSSSDSSLQSQSSIDAKY
                     INLTMVTPKTINPITNTDKSVGYIMNLVYDSLFTIDENYNVIPQLVKEYNIAQDGMSI
                     DIKLKDAKWHDGKNITSNDVNYTIGLIQKSADSPYNEFTKNIASVNIKSDKDFTIKFK
                     ARYAFSIDSLIFPIVSQNHLDSKDVNDKNNNMIGNGKYKIESYTEREGMVLSVNKDYY
                     EEVPKTMKNIKVGMVPNEDARTSMVMALDSDITNVTLNDLSKFQEKEFNITKYQGRDY
                     ECVLFNYNNPFFKDVNFRKAIAHSIDKDRIISEGYMDDATPVNFPLNSKSKYYNSEMK
                     DLEFNKDKAIECLAKVEYANVNSVNQNDNKVKNQKKSAKKNSKKLTPEEEAKAKKAEE
                     DRIKKEAEEKKNREKEEVKKSLSEMNLKIIVNRDNSERIKTANIINENLKAIGINSTV
                     NQLSDKDMENALNSKNYDLALVGWKLSIIPDASSIIASSGYTDDKLNGYMSALTSSTS
                     EIETKKAYKDVQTYIKDNATFISLAVRNNYIVSNSRLKGKITPNDFDVYEGISNLDIK
                     SNESN"
     gene            9151..9363
                     /locus_tag="KR250_000123"
     CDS             9151..9363
                     /locus_tag="KR250_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HBH0066095.1"
                     /translation="MKKKLLVEGMSCGHCVNHLKTALTEDIDGIEVLDVDLENKCASV
                     DMKDDVSIEKLKEVIAELGFELKGIE"
     gene            9564..10163
                     /locus_tag="KR250_000124"
     CDS             9564..10163
                     /locus_tag="KR250_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0066096.1"
                     /translation="MESRGYKEDLKKMILDMAHKELESYVSQKGAKKYFEGNLDKIIN
                     NNIKKFNQSIGLNRVYLKMLKKCAYQESVTSISRTIKKEALFKSKKELFAFSEYLKLD
                     INKRLSYNQILRKISRYIYYNRSSYAQKYVVFKRGDEEYLLEPEKIKDELISSYRSKT
                     REDMRSIAKLLDIEVEDDYNAEDIRKKVINYIIKEKLKS"
     gene            10391..10762
                     /locus_tag="KR250_000125"
     CDS             10391..10762
                     /locus_tag="KR250_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBH0066097.1"
                     /translation="MKFNFCHNNFNVTNLEKSLDFYKKALGLKEVKRKEAEDGSFILV
                     YLGDGITSHTLELTWLRDWDRPYNLGDNEFHLALEVEEFDEAKKLHKDLDCICFENES
                     MGIYFIADPDNYWIEILPKNH"
     gene            10894..12168
                     /locus_tag="KR250_000126"
     CDS             10894..12168
                     /locus_tag="KR250_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HBH0066098.1"
                     /translation="MKKAIAALGIGAVAVSVSSINASALEKGTVTASALNIRSGPSSD
                     CDKVAKLYKGKTVEILEKSNGWYKVRVSSSVVGWGSAKYISTSGSSEGTSSQNNPTSS
                     GTTISGNGKVNVSSRLNVRSGAGTNYSLVGKANNGDVVKLLEQSNGWYKIKLSNGVTG
                     WASSQYISKTSEDVGTNNSSNSNSTNNSDKKPSSEESIEGKNGKVTSAVSLNVRSGPG
                     TSYSIIGKLNGGDVVELKAKSNGWYKVKLSSGTIGWVSASYISETNEDTKEKPNSSSN
                     QNSQSNSNSKPSFTGNSDKSTAKGSTIVDFAYTLIGIPYQWGASGPDKFDCSGFTQYV
                     FKHSVGVSIPRVSREQANFGSAISMGNYAPGDLVYFDTDGDGTTNHVGIYVGNSKFIH
                     CSGTQTNPNKVKVDNLTSSYWSKVLLGARRFV"
     gene            12279..13223
                     /locus_tag="KR250_000127"
     CDS             12279..13223
                     /locus_tag="KR250_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0066099.1"
                     /translation="MNRRKTIYRGFNNNRHKIDLRKYVITIACLCLIGYYSYTKIKDS
                     KILEYVSAKIPFLNNSSDITYKDISDELNSIKKGKKSKSQTNSDDKQETNPEKAVNNT
                     KEPEEVKLATIEGWDMYTIQVAAIDNNDDLKKIQTSLVNNDIPFSVMEKDGVKKIQTY
                     SSFDENDVRKQISSVRKVFPDAFLSHLDAPMLSLEYTSNYAYIESISKELNKLITNFK
                     EESSFWSNAENNVDMEKYNTILTNRKAISQNISKEAEKIDYSEMRLFKDNLIEYVKNV
                     NEKIDTASKAANEEKYSVSKSLLLSSMQEYSMFINSIK"
     gene            13439..14776
                     /gene="rsxC"
                     /locus_tag="KR250_000128"
     CDS             13439..14776
                     /gene="rsxC"
                     /locus_tag="KR250_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HBH0066100.1"
                     /translation="MKLLTFKGGIHPPYRKEYSNTKALEKAQAPKIVYIPLQQHIGAP
                     AKPIVEVGDEVKFGQKIGEQQGFVSCNVHSSVSGKVIAIEQHEVPGGSAQCVVIENDF
                     KEELHESVQPKGQLEDLSKEDIVGIIKEAGIVGMGGATFPNHVKVSPPPDSKAEVVIL
                     NGAECEPYLTADHRLMVENPEDVVFGLRALMKVLDVKKGFIGIETNKPDAIEAIQNVA
                     KDYSEIEVVGLQVKYPQGAEKQLIYACTGKEVPSGGLPIAAGAVVDNVATAAQIAKSI
                     KTGMPLVERITTITGSCIKEPKNLITKVGTLVSEIIEQCGGFKEDKKIGKVIMGGPMM
                     GIAQYTTEIATNKGSSGILCLDEEESRTPDIQNCLRCGRCTDVCPSFLQPLFISAYSL
                     KDDYDTAEYHRAMDCIECGSCSFICPARRPLLQSIRSAKREIGAKRRKQAAQK"
     gene            14799..15776
                     /locus_tag="KR250_000129"
     CDS             14799..15776
                     /locus_tag="KR250_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HBH0066101.1"
                     /translation="MENKLIVSSSPHVRSNEDTSYIMKQVIIALLPAAVAGVYFFRLN
                     ALSAMFFCILGTVGTEFLYQKFTKQKSTIGDFSAVVTGLLLAFNVPASLPWWMCLVGG
                     IFAILVVKMVFGGIGCNFVNPALAARAFLLASFPVAMTAWTQPGVNWIGKNLDAVTTA
                     TPLSFLKNGAAGLADISSNGISLADMMIGNIGGCIGETSAILLLLGGVYLMYKGIINY
                     VIPVFYIATVFILTFLLGGFNITFAIYQLFAGGLMLGGFFMLTDYTTSPMTKKGQIIY
                     AVLAGLITTVIRMYGGYPEGVSYSILLVNCLAPLIDKFVRNRVFGEVAK"
     gene            15776..16345
                     /locus_tag="KR250_000130"
     CDS             15776..16345
                     /locus_tag="KR250_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit G"
                     /protein_id="HBH0066102.1"
                     /translation="MNSMVRLGGTLLAISAIAALALGATNQVTAPVIEQRNIQANNEL
                     RKAVLPEAKEFKEMKEDTYKGLGDGLIAEVYEGLDGSEVVGYTLKAKPSGYGGEIEVM
                     VGISSEGQVTGVDIGNMSETAGLGAKAKDDAFKGQYKGKTAEPLEVAKGSTTADNQIL
                     AISGATITSTAVTTGVNAAIDVFNSALNK"
     gene            16358..16951
                     /locus_tag="KR250_000131"
     CDS             16358..16951
                     /locus_tag="KR250_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HBH0066103.1"
                     /translation="MNLAKVFKNGLIDENPTFVQVIGMCPTLAVTTSAINGIGMGLST
                     AAVLICANLVISLIRKITPDKIRIPIFIVVIATFVTIVGMLLKAYVPALDKALGIYIP
                     LIVVNCLILARAESFAFKTGAMPSIVDGVGQGLGFTVALTIIGAVRELLGNGSLFGMT
                     LFGASFQPVLIFILPPGAFLTLGFLFAGFNKLRSKKA"
     gene            16966..17541
                     /gene="rsxA"
                     /locus_tag="KR250_000132"
     CDS             16966..17541
                     /gene="rsxA"
                     /locus_tag="KR250_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HBH0066104.1"
                     /translation="MNLILLFLSIVLVNNVITSQFLGICPFLGVSKKVDTAVGMGVAV
                     TFVLTLASIITYFIQILLVKTGTGFLQTIAFILVIASIVQFVEMVIQKMSPSLYQALG
                     VYLPLITTNCAVLGIALVNVQKSYNLVETIINGFGAGAGFTLAIVIFAGIRERLELAD
                     IPEAFKGFPITLISAGLMSIAFLGFTGLIKL"
     gene            17557..18528
                     /locus_tag="KR250_000133"
     CDS             17557..18528
                     /locus_tag="KR250_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HBH0066105.1"
                     /translation="MVILTAVLVLGIMGLIFGIVLDFASKKFAVEVDERVEAILGVLP
                     GANCGGCGFPGCGGLANAIVEGNAPVNGCPVGGADVGAKVGEIMGISAEAGEKQVAKV
                     ICKGTCSSAKDKYEYEGISDCRAANVLNSGAKMCKFGCLGLGTCKDACKFDAISIVDG
                     IAVIDEEKCVNCGKCKEVCPKGIIITKPESQEVVVECNSKEFGKAVKEKCTAGCIGCG
                     MCVKACKFDAIIFEDKIAKIDPNKCVGCMQCVAKCPTKVISGDITKKKKVTIDQELCV
                     GCTVCKKQCKFDAIEGELKEKHKVDADKCVGCHLCMEKCPKKAIKIL"
     gene            18748..19332
                     /gene="maf"
                     /locus_tag="KR250_000134"
     CDS             18748..19332
                     /gene="maf"
                     /locus_tag="KR250_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HBH0066106.1"
                     /translation="MNIILASASPRRKEILENTNVRFDIIKNEIDEIILEGETPKHLV
                     MRLAFEKSMSVASEHNEDVVIGADTVVVLDNAILGKPKDESCARDMLKRLSGREHQVI
                     TGISLINLCEDKKVIDYVISNVKFKTLSEQDIEDYLKTNESFDKAGAYGIQGYGALLV
                     EEIRGDYFNIVGLPISRLGDLLKKYFSINLFYGV"
     gene            19377..20036
                     /gene="radC"
                     /locus_tag="KR250_000135"
     CDS             19377..20036
                     /gene="radC"
                     /locus_tag="KR250_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HBH0066107.1"
                     /translation="MAPEERPREKMLAKGVKSLSNAELLAILLRTGNKNKNAIELANY
                     IINRDIQGIRHLEDMTIEELCNIDGIGLSKSTQIKAALELGSRVASFKPIKYKIRNPW
                     DIQRYYMDSLRYLKKEVFKAVLLNTKNEIISDVDVSIGTLSSSLVHPREVFKEAIRRS
                     ASKIIVMHNHPSGSVEPSREDKNITSRLIKCGEIIGIEIIDHIIIGDGLYFSFKENMI
                     I"
     gene            20157..21167
                     /locus_tag="KR250_000136"
     CDS             20157..21167
                     /locus_tag="KR250_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HBH0066108.1"
                     /translation="MTKDMGIDLGTANTLVYIKGQGIVVREPSVVAIRDDSKEVLAVG
                     EEAKKMIGRTPGNIVAIRPMKDGVIADFDITQSMISYFIQKAADKKGVVSPRIAICVP
                     FGVTEVEKRAIEEAARQAGAKDAFLIEEPMAAAIGAGLKVEEPEGNMVVDIGGGTSEI
                     AVISLGGIVTAKSIRIGGDEFDESIVAYVKKEYNLMIGERTAENVKINIGSTFKDDEE
                     INMQIRGRDLISGLPKTIEICSTEVREALKEPVSSIVDAIKSTLERTPPELASDIMEN
                     GIMLTGGGALLRGLDKLITQETGMSVQIAETPLDCVALGTGKSVEDQEIFEKVLMMNT
                     KN"
     gene            21177..22070
                     /gene="mreC"
                     /locus_tag="KR250_000137"
     CDS             21177..22070
                     /gene="mreC"
                     /locus_tag="KR250_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HBH0066109.1"
                     /translation="MALRFDKNKNEKKRINVKVIATGVVAITLIGIVGISIGKFSSGS
                     PVNLGVASDAITSVGKGINDGFSFIKNGFKDVANFKDNSKKVKKLEEENEKLKKNMIA
                     LNAKLDKTESLEELKKTLNFVQEEYKATSISASVVGKNDGNWYESFVIGAGKNSGVKK
                     ESIVMNGSGLVGIVYEVSNNYSKAISLLDSKASVSFKLAKDANAKGTITQNTTLDNKD
                     SYNSKGYLQGYMFDSSYNVIQGDIITTSGLGFFPDGIPIGEVEKVVDDKDKSLKYVVV
                     KPYVDFKNINDVVVIEPRNIG"
     gene            22085..22573
                     /gene="mreD"
                     /locus_tag="KR250_000138"
     CDS             22085..22573
                     /gene="mreD"
                     /locus_tag="KR250_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HBH0066110.1"
                     /translation="MKKVLLCLLGIVLMTIENSITNYIDIFGISFNLVLIYVTIISLY
                     LDELEGGIIAAIIGLIKDITVGGIFGVNALILFIIAYAIGYMRDQLYKGSYIITFVLI
                     FIGSLIDSIVNIGTSSIIYQSYNISTLFVKGLLVAPIVNSLIGLVIYRLSKRAVLKLK
                     ED"
     gene            22585..25563
                     /locus_tag="KR250_000139"
     CDS             22585..25563
                     /locus_tag="KR250_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HBH0066111.1"
                     /translation="MEENKQVDRLKIIKIVMGVIFLAILVKIIYMTTFKYEYYNELAE
                     NKTYKKLAIEAPRGEIKDRYGRLLAGNKNLFTVQVSGNDINKKDANKHSRANEISLKL
                     INLLERNGEEYVDEFPIYVENGKYYYTYDRDIREYKSENGIPNDYNAKESFYYLVDKL
                     ISAGILSQEDKRLDATRLQAKLNENGYYPPILVSKWMFTAERDKRDWLASYKIKETKL
                     SAKEAFEKVRNSDALEIDKSLSDEDARKIMVVRDLIKSKGYSQYNPVTIAKDVGETTI
                     AQIEESAMDLVGVSIAVEPVRYYPNGSLASHMLGYVGKMPSTQIESYLQKGYETGDMV
                     GLAGVEKSNESRLRGTDGYKMVKVDALGRISKEIESKKPKSGDTVYLTLDKDLQEVSD
                     NALKQIIEVASKGGTFKSKFGDKPISAYAGKAQSAALIAIDVKNGEVLASSSYPNYDP
                     NKFAKGISTEDYKALQPKNPNDLLAGSPLLNLVTQGEFQPGSSFKMLTSMAALENGLD
                     PNFTINDPGVIMLGKKSFGDYVWNHGRGNHGMTNLYKAIQESCNIYMATIGTGKTWPD
                     GKSIGIDMNANKILEYAKLFGLDQNTGLQDEVEERAGKVPSTEDKLKSTQALLKSNLE
                     REMANDFVDITREKNPKEYEKRINEIVSWAAEKKTPGRVETMNRLKKLDVKEDRIEEV
                     ADLAVFSYFNFAKWSTADTFNLAIGQGENAYTPAQISRYVAAIANGGNLVELSVVDRA
                     VSSDYSSVKINDQKKVEKIPFKNPDNLKELTKGMKLVVRQGTAKSAFADFPIDVAAKT
                     GTAEKSGKIPTDNEYEYLKSHMSSYNVNLNDAIKLADKMKAEKEKELSLAKEKEIKKK
                     LENKDLKDEERKKLEEELEDGVKVRLEDTDKVNSSYLRKAIKELNPKITDDQIDRFKQ
                     DYGSFTWTVAFAPADDPEIAVVCVIPQGDSSVFSLLPTREVIGTYMGLEPTNSKNDNK
                     TNDVNNSSDENINFESQINR"
     gene            25661..26344
                     /locus_tag="KR250_000140"
     CDS             25661..26344
                     /locus_tag="KR250_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HBH0066112.1"
                     /translation="MSLREICSQELVEFKGNKRGIIVNIKREAPFEEIQEKIINKLEA
                     YVGFFNGAKISKINSDCLTDMEILELKEGITSRFDVEFVEDQKIEENSNFPTKYVNTL
                     RSGENIEFEGDVVILNDMKPGSKVLSKSNTVVMGDINAGAKVVAGGNVFVMGKIEGFV
                     HAGAEGNEFAYVVAGNLNPKILQIADNIAEAPDDEENYESESEISPEIAFVSNGRIVI
                     ESYLSKLDK"
     gene            26368..27165
                     /gene="minD"
                     /locus_tag="KR250_000141"
     CDS             26368..27165
                     /gene="minD"
                     /locus_tag="KR250_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HBH0066113.1"
                     /translation="MSEVIVITSGKGGVGKTTTAANLGTALSLENKKTVVVDADIGLR
                     NLDVVMGLENRIVYDIVDVVEGTCRLKQALIKDKRFDNLYLLPAAQTRDKNAVSVEQM
                     IDLCEKLKESFEYIIIDCPAGIEQGFKNAVAGADRAIVVTNPEISAVRDADRIIGLLE
                     ANEIKEIRLVINRIRNDMVKRGDMMDKQDIIEILAIDLLGLVPDDESIIISTNKGEPA
                     ILDSKSLAGQAYKNIAKRILNEEVPLLDLEVEDGFFGRLKKMFSMAK"
     gene            27183..27467
                     /gene="minE"
                     /locus_tag="KR250_000142"
     CDS             27183..27467
                     /gene="minE"
                     /locus_tag="KR250_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HBH0066114.1"
                     /translation="MLDLFRVFSNEAKTSKSVAKERLKLVLVHDRVDCSPQLLEMIKT
                     DILKVIANYAEIEDDGLEIKMSKCRGEHDDKPVSALVANIPLKNIKDRCM"
     gene            27552..28682
                     /gene="rodA"
                     /locus_tag="KR250_000143"
     CDS             27552..28682
                     /gene="rodA"
                     /locus_tag="KR250_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HBH0066115.1"
                     /translation="MKKININYKSTIKLIKQLDWKLIVTVLAIFIFGLVILSSATHAN
                     STGSYNQLIKQGLAFVLGIGMIIVILFFDYNLLGRYYKALYIISLILLAIVLLPGIGT
                     VKGGARSWINLGPLDLQTSEIVKLTFVLSYAKILESKKDKLNTLKEVMPVVVYSLPFI
                     GLLIAQPDLGTGIVFCCMIFAMLFTAGLSSKLIKRGIIILLVSMPLMYLMMADHQKVR
                     IEAFLNPEDVTLKGNYQVMQSLIAIGSGGVTGKGLYNGSQNQEDFLPVQDSDFIFAVV
                     GEELGVIGMAVLIILFMIFLLRLLAIARDAKDFYGTLIVVGVMGMFGYQIIQNIGMTV
                     ALIPVTGVTLPFVSYGGSSLLTSLANLGLVLNVCMRRKKINF"
     gene            28723..29136
                     /locus_tag="KR250_000144"
     CDS             28723..29136
                     /locus_tag="KR250_000144"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HBH0066116.1"
                     /translation="MNIALVAHDQMKNTMVGFCIGYESILKKYGLYATGTTGKRIMDE
                     TELNINRLASGPLGGDQQIGSLIVTQEIDLVIFLRDPLTSQAHETDIQALIRLCDVYH
                     VPIATNLASAEIFIKALDRGELSWREVRKSKSQRI"
     gene            29621..29950
                     /locus_tag="KR250_000145"
     CDS             29621..29950
                     /locus_tag="KR250_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0066117.1"
                     /translation="MQLNKEVLKGYIDILIVSILEKKDCYGYEIAKQVRERSEFELKE
                     GTMYLALKRMESKNLIKSYYSNEQSSGGRRKYYNLTNEGKDFLEIKKQEWRFIKKVMN
                     QFLGEVD"
     gene            29954..30745
                     /locus_tag="KR250_000146"
     CDS             29954..30745
                     /locus_tag="KR250_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0066118.1"
                     /translation="MKLIDEYLDKLYKKCDNKSTIELKQEMRCHLIESANEFKLEGLD
                     EEEACKKAIERFDDGDEMQYELCNIIKELSLSLDRHKSIVMGFKKVLGYISIIAFLIS
                     GFMWYYNNSLQHNMYNLGKELDGEIKQLAERHDMTKIGEYKLELEKILDKDKYSKVKA
                     LRLYVIDMKDGNTNLSSSGLNANMVYEREADYNNISNFIQHLGYNGKDFLDKNGNIVN
                     PDIFLEYFFYFESEMLIPVAFAFGLLCIIAYFILRFKISLIKNNN"
     gene            31309..32391
                     /locus_tag="KR250_000147"
     CDS             31309..32391
                     /locus_tag="KR250_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HBH0066119.1"
                     /translation="MKKIRKLATKLLIAMMVLTSCMSVTVYGMTAKDVTAKTPKSYVA
                     GTNSIYGPKLSQAQLNSVAQATADFMNKKITKNMTTDAKIRVAYNHIKNNTTYVNWNA
                     AEGANTAYTLVTKKGACSGMCRSLKALCDAMGIECYYVHSTTNDHQWNLIRFEDGRLY
                     HVDIDANKSAGADIIYKSLTHPLPFDKKAYPAVGEVVKTQNPTPTVKKPTPAKPSAPV
                     GPGNHTMIADQQGAILDEVYGNNLRPKIEMYEGVPCSLINSSINTIKFTSKNSNLDPS
                     KHTVTVIGFMNTPYGMVALAPDGVPDEYPFEYDKPFSLDFTGKNLSAAELSKKCKENN
                     VIIKVEIGETASNSGRGMVAYARYVN"
     gene            complement(32603..33796)
                     /locus_tag="KR250_000148"
     CDS             complement(32603..33796)
                     /locus_tag="KR250_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HBH0066120.1"
                     /translation="MSKVFEVKKDIYFTGVVDEGLKVFDIIMETEFGTTYNSYLIKDE
                     KTVLFDTVKANFKDEFLSNLSEVTDIAKIDYVVIHHTEPDHAGSLKYLLDINPNIEVY
                     CTKAAKLYLDGQINRPFNCHVIKDGEILNIGKRNLRFITAPFLHWVDTMFTYIEEDKT
                     LLTCDAFGCHFASVDAEVVNSEDYLKSAKHYYDCIVKPFAKHVLSAVDKVVGLNIEFD
                     TILTSHGPMLTKDPMAAVKRYVEWSTEAVNTTNQNQVSIFYLSAYSNTLEMAKKIKEG
                     LDKEGAKAELYDLEDMTLTEMHDTLVVSKVILLGSPTINKTMVKPMWDLFSVIDPMAN
                     QGKIAGVFGSFGWSGEGITMAETLLKSMSFKMPVESLKKKFFPSEETLKECMAFGAEF
                     AKLVK"
     gene            34115..35959
                     /locus_tag="KR250_000149"
     CDS             34115..35959
                     /locus_tag="KR250_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HBH0066121.1"
                     /translation="MNKVDLKKILKKVEKPARYLGNEINSIHKDTSNSELIRYAHCFP
                     DLYEVGMSHLGSHILYDVINKDEDVFCERVYSPAVDMENIMREKSIPLFALESREPIT
                     NFDFVTFTLQYELSYTNILNILDLANIPILKEERTLEDPFIMVGGPCAYNSEPLADFV
                     DVVILGEGEEVNLEVVNEYKEWKKNKTTREDFLYKISSIEGVYIPSFYDVKYNEDGTV
                     QSVTPNREGITKNPTKRIIKDVETVDYPEKLIVPYIDTVHDRIVLELFRGCTRGCRFC
                     QAGMIYRPIREKSVKRLKEILDKLVKNTGYDEISLSSLSTSDYSKLSELTDYLVDEYA
                     SNNIGISLPSLRLDNFSMEIADKIQQVRKSGLTFAPEAGTQRLRDVINKGVTEEDLEN
                     ATERAFEMGWNSVKLYFMIGLPTETYEDLDGIAKLAYKVIDIYRKVNGGKLKRSFSVT
                     VSTSTFVPKPFTPFQWHGQDTTEEVINKQRHLVNKLRNNNIKYNYHDSKTSLMEAVVA
                     RGDRRIGKVIYDAFRLGAKFDGWAEHFNLDIWKEAMEKNNLSIDFYAHRNRNYEEVFP
                     WDHIDVGISKKFLIREDENAKKEKITSDCRHNCNGCGINIHDIGRGLC"
     gene            35961..36662
                     /locus_tag="KR250_000150"
     CDS             35961..36662
                     /locus_tag="KR250_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2344 domain-containing protein"
                     /protein_id="HBH0066122.1"
                     /translation="MSKIIRVKFKKEGDMIYISHLDLQRLLQRAFRRAEINLSHSQGF
                     NPHPKMSYGNALALGTESQGEYVDIEIEEDDLSVEEFLNKVSIQLPDGIDFIKAKEID
                     RQTPSLSSVIDYGEYLFNIDLKRPMTKEFVKRKVIDFMNNKEIIITKKNKKGKMVEVD
                     IRPMIRTFDVLNLEDEHITLTATIATGSKTNLNTNILIPKILEMFELDIDPLDVDILR
                     RDLYVLEDGELVTPM"
     gene            36680..38101
                     /locus_tag="KR250_000151"
     CDS             36680..38101
                     /locus_tag="KR250_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HBH0066123.1"
                     /translation="MKKIVIESLIGSQKTAVLEDERLTELFVEDNLNKKTVSNIYRGI
                     VKKVIPGIEACFVDIGFKKLAYLQLKKGSTIKSGQDILVQINKEEIGTKGAKLNTEIS
                     ISGRYIVYIPSNDRTTISNKITDEKERFRLKKITKAVNKENLGLIIRTEAQGCTHDEI
                     KKDIEELKLKYENILKEYKLGIGPKLLYKSLDFATKYVKDNVNDDIESIITNSYDKYS
                     ELKSILRGIDKTYVDKLCLEENRDVFDLYRIESKIEKLLNKKVWLKSGGYLIIEKTEA
                     LTVIDVNTGKFIGTGKLDETVYKTNLEAAKEIVRQLRIRDIAGIIIIDFIDMHKKKHQ
                     KEILHILEEEFNKDKRKAEVLGMTKLGLVEVARRREKESIDKYYLMSCPCCDGEQTIK
                     SVHYILDSIEKEIMRISEHTVYKNIMVEFNDFIFEQIKEYYMDIIDKIGEKYNIKISL
                     NANSTLKHNKTNVIFDKIVDNKM"
     gene            38202..38513
                     /gene="rplU"
                     /locus_tag="KR250_000152"
     CDS             38202..38513
                     /gene="rplU"
                     /locus_tag="KR250_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HBH0066124.1"
                     /translation="MYAIVKTGGKQYKVSEGDVLFVEKLEANAGDVVTLNEVLACSKD
                     GELKLGSPVVEGASVQAKVVEQGKAKKVIVFKYKAKKDYRRKQGHRQSYTKIVVEKIN
                     A"
     gene            38528..38845
                     /locus_tag="KR250_000153"
     CDS             38528..38845
                     /locus_tag="KR250_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HBH0066125.1"
                     /translation="MIKVKYYYNDDFLLQGFCLKGHADFAEIGYDIVCAAVTSNAIAV
                     INSLDKLQKIEFEKVVGEEGHIECIVKDTHVKDAQLLLNHFQLAVKEIKREYPKNIKI
                     LKK"
     gene            38859..39149
                     /gene="rpmA"
                     /locus_tag="KR250_000154"
     CDS             38859..39149
                     /gene="rpmA"
                     /locus_tag="KR250_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HBH0066126.1"
                     /translation="MLNMNLQLLASKKGVGSSKNGRDSISKRLGVKRFDGQLVTAGSI
                     IVRQRGTKIHPGTNVGKGSDDTLFALVDGTVKFERKDKKRKKVSIYPVAIAE"
     gene            39260..40537
                     /gene="obgE"
                     /locus_tag="KR250_000155"
     CDS             39260..40537
                     /gene="obgE"
                     /locus_tag="KR250_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HBH0066127.1"
                     /translation="MFIDKARIFVKAGNGGNGSVAFRREKYVPAGGPDGGDGGRGASI
                     IFEVDLGLRTLMDFKYQKKYQAQNGGDGSKGKRAGKNGENLVLKVPAGTVIRDEATGL
                     VLADLKKEGDTAIVAKGGIGGKGNQHFANAVRQAPAFAKSGTDGEERWITLELKMIAD
                     VGLLGFPNVGKSTFLSVVTKAKPKIANYHFTTLTPNLGVVQTKFGDSFVLADIPGIIE
                     GASEGIGLGHEFLRHVERTKVLIHIVDISGLEGRDPIEDFDKINDELKLYNEKLSKRP
                     QVVVANKFDILEDESKFEKFKSELEGRGYTVFKMSAATRQGIDEVIAYVSKMLKEVED
                     VELVSEEEMYRPELDIGTEEELSIDIEDGVYVVTGKALRRIMYSVNFDDMESLQYFQK
                     AMESQGVFDRLREMGIEDGDVVKIYELEFEFYN"
     gene            40589..40957
                     /locus_tag="KR250_000156"
     CDS             40589..40957
                     /locus_tag="KR250_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbY family RNA-binding protein"
                     /protein_id="HBH0066128.1"
                     /translation="MLKGKQRAYLRSLANTLKPTTQIGKEGVTESFLEQLDGMLRTRE
                     LVKVTILENAGLDTKETANAVCEALRAEFVQAIGFKFTIYKRNIEEPKILFPGHEQAK
                     AKTKNNNVTKKGKPTKRAVR"
     regulatory      41049..41155
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            41258..42463
                     /locus_tag="KR250_000157"
     CDS             41258..42463
                     /locus_tag="KR250_000157"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HBH0066129.1"
                     /translation="MKIAILGYGTVGSGLIDLIENNTSKRDIEVVGILVKNKEKHKYK
                     KYFDKITTDIEDIFDKDIDILVEVIGGLNPAFDYVTRALNKKIHVVTANKDLLAEKGS
                     DLIELANLNNVSIKFEASVAGGIPVLKPLIESLEGNNIKSINAILNGTCNFILSKMYD
                     ENLPYDVALKQAQELGFAEVNPDADVLGYDSARKLSILSTLSYGKIVYWKDLLLEGIT
                     SIDEKDIEYAKKLNCKIKLVARSKYESGEVSGFVRPALVDNNNMLSKIDNEFNVVILV
                     GDSVGELSFVGKGAGRGATGSAVYADIIDIIDNRSSNIKSFSKGKLDLSGLIEDECSA
                     VVRFGGYNNKDGILECLDKYVDSYDIIDDEELAIFVSAKSEHEIDRVLEIVKRNNYSE
                     SAKKLLKID"
     gene            complement(42664..43230)
                     /locus_tag="KR250_000158"
     CDS             complement(42664..43230)
                     /locus_tag="KR250_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBH0066130.1"
                     /translation="MATRPIEIDEYKKIMELLHTGFTYSENGVEKRFRKNPKVALALM
                     LEANLGLRISDILRLKIGNFKGNMLEINEKKTGKLQYRPVNKNIIESINEHARKYNLK
                     SNDYLVNIKTKAIQKQLRIICKHLNLYNISTHSFRKLYATTQFEKSNNNLELVKELLN
                     HSSVATTQRYIRVTQQAINEASENFFIG"
     gene            complement(43510..44769)
                     /locus_tag="KR250_000159"
     CDS             complement(43510..44769)
                     /locus_tag="KR250_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YlbJ"
                     /protein_id="HBH0066131.1"
                     /translation="MKKNKIIAFFAPSIIVGVLVLGIILYPNEAIKSAREGFSIWSNV
                     LVPSLLPFIIGANLIVDLKIVDIIGFIINPITRFVFNVSGKSALAFAISTVSGYPVGV
                     SLASEFRSNGQISKYEAQRLVSFCSTSGPLFIIGSVGTGMFQNSNLGYLMILCHYLGT
                     ILVGLFFRNYGNENLPKTKLDLKTNIKNVINIRNSSGNGFFVLFGNAVFNGVNTLLTV
                     GGFVIVFSVVFKILSLFNVISLIASVIYLPLSFLGVSKELCQAFVSGLFEITIGCNKV
                     SSITSSPEILRASLASFLIGFSGLSILAQCCTFLSKTDIDLKLYILSKFAHGVLAAIF
                     IFILYPIANSAVLVSSFADTYNVIYNNLIWFYYLSYYDTILQIVVIIYLISAVFIAKK
                     HMKTLSTNGKLMRYKNTFFTKKFSRKK"
     gene            44894..45607
                     /locus_tag="KR250_000160"
     CDS             44894..45607
                     /locus_tag="KR250_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HBH0066132.1"
                     /translation="MFNSIASKKVYEQVIEQIQYKILNGELKKGDKLLSERELSEQMN
                     VSRTSIREAIRVLETMGVIESRQGEGNFICTNIEKTLIEPLSMIFKLNNGTLEDILEL
                     RIILEIEIAKLASKRITSSEVIELKHIIDEMRVETNKKDNNRVLVLLDQKFHSKLATL
                     SKNYLIQSLFMTASKLFDGFIEDAREKIIAEPFNENILLKQHEAIYNAVVENDVELAC
                     EKAKEHMDFISKNYRKNEN"
     gene            45725..46999
                     /gene="larA"
                     /locus_tag="KR250_000161"
     CDS             45725..46999
                     /gene="larA"
                     /locus_tag="KR250_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HBH0066133.1"
                     /translation="MVKLKVPYSKQGMILEIPDERFLGVLESKSDNYIEHKTQEEIVE
                     ESMNNPIGSNSLEELVKGKKNVVIITSDHTRPVPSRITMPIILRRIRNVNPEIDVKII
                     VATGFHRPSTREELIYKMGEEIVDNEDIVMHISTDDKSMCKVGILPSGGDLYINKLAY
                     EAELLIAEGFIEPHFFAGFSGGRKSVLPGIASAKTIMYNHCSDFIDSDNSRTGKLNNN
                     PIHEDMVYAAKVAKLAFILNVVIDKDKKIIASFAGDVEKAHTKGCRFVTELSKVNSIK
                     SDIVVTTNGGYPLDQNIYQSVKGMTAAEATCKDGGVIIMISACNDGHGGQSFYENVAN
                     ADSPKQLLDKIRSVDRVDTIPDQWEFQILARILSKYTVIMVTDKCNPEMIKKMHMKHA
                     FNFKEALEMANSIVGKESKVVVIPDGVSVIVK"
     gene            47016..47804
                     /locus_tag="KR250_000162"
     CDS             47016..47804
                     /locus_tag="KR250_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003402402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HBH0066134.1"
                     /translation="MEILTCIKQVPGTTSVEVDETTGVLKRDGVDSKMNPYDLYALET
                     ALRIKEDKKANLKVLSMGPPQAKKVIEESFMMGADEGALISDRRFGGADVLATSYTIS
                     QGIKKMGKVDLIICGKQTTDGDTAQVGPEVAEFLDIPHVTNVTKLIEVKDESIVVEID
                     MPNDLQVCEIEYPCLITVEKDIFQPRLPSFKLKLNTKDREIPVYSLDDFEDKNENNYG
                     LNGSPTQVVRIFPPKPNTDKNIVRGNADELSFALVNKLEELKLV"
     gene            47818..49038
                     /locus_tag="KR250_000163"
     CDS             47818..49038
                     /locus_tag="KR250_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HBH0066135.1"
                     /translation="MSKIVVNQDKITDLKRILEICPFGAIEEKSGIVEISAGCKMCKL
                     CVKSGPKGAFEFIESSKVQINKDEWRGIAVYVEHHNGNIHPVTYELIGKAREMASKIK
                     QPVYCVFVGKDIKDKCSNLISYGVDEVFVYDEDEFKDFRIEPYSKAIENFIDKIKPTI
                     VLVGGTTLGRSLAPRLAARFRTGLTADCTILDIQSNTDLDQIRPAFGGNIMAHINTPN
                     NRPQFATVRYKIFSAPEKIENTTGKITLCKVEKKELKSKIKVLSVKEKNKEVGLEEAE
                     VIVVASRAIKKQEDMEMIYKLADKLNAQVAGTRPVIEAGWIDAKKQIGLSGRTVKPKL
                     IITCGVSGAVQFVAGMQGADYIVAINKDDKAPILDVAHLALIGDIYDIIPKLIEKIEN
                     NKDNNQKYMASAAK"
     gene            49049..50440
                     /locus_tag="KR250_000164"
     CDS             49049..50440
                     /locus_tag="KR250_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HBH0066136.1"
                     /translation="MYKLIDKKDIDFLIDTCGEENVLVGSDINEDFSHDELGGIEKYP
                     EVLVNVLETEQVSKIMKYAYKNNIPVTPRGQGTGLVGAAVAINGGIMINLCKMNKILE
                     VDYENLTLTVEPGVLLMTIGQYVQDRDLFYPPDPGEKSATIAGNINTNAGGMRAVKYG
                     VTRDYVRGLEVVLPNGEIINVGGKVVKNSSGYSIKDLLVGSEGTLGIVTKAILKLLPL
                     PKKSISLLIPFPDLSMAIETVPKIIKSKSIPTAIEFMERDVILAAEEFLGKKFPDNTS
                     DAYLLLTFDGNSTEDIEKEYEKVANLCLENGALDVFISDTQERNDSIWSARGAFLEAI
                     KASTTQMDECDVVVPRDKIAEFIRYTHELQDKLKIRIKSFGHAGDGNLHIYILKDGMD
                     DNTWKIRLKETFDYMYKKSRELSGQVSGEHGIGYAKKEYLHESNSDAYMMLIKNIKLA
                     FDPKNILNPGKIY"
     gene            complement(50703..51983)
                     /locus_tag="KR250_000165"
     CDS             complement(50703..51983)
                     /locus_tag="KR250_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HBH0066137.1"
                     /translation="MNILGLIVEYNPFHNGHIYHLSKSIEKTNATHTVAIMSGNFLQR
                     GEPALFDKYTRAKIAVENGVDLVIELPTLFACQSAEIFSHGAITILNSLNCINSICFG
                     SEEGNTEILYNIAEILANEPNEFKLLLKKYLDDGLLFPTARANALFDYIHQDKKNNFI
                     QNYSKEQLSKTLNSSNNILGIEYIKNLIHLNSSIKPFTISRVQSEYNSEEIESDICSA
                     TAIRTSLKENTDLFHLKNVIPEKTYNILNEKIDNGFLPMFDDFFFDILKTIILRDMDT
                     LKEYFEVNEGIENRIYKDIFTSEFLHELQLAIKSKRYTLTKIKRTLNNILLGIKKSDM
                     QLIKNIDTIPYIRILAFNDKGREILKEIKNKSEIKIINKFSNISFSLDDTIFKTLISY
                     DIKATNIYNSIYYKNNKDLVKGPMDFYTHPIYVK"
     gene            52446..53642
                     /locus_tag="KR250_000166"
     CDS             52446..53642
                     /locus_tag="KR250_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HBH0066138.1"
                     /translation="MKILVLNCGSSSLKYQLIDMNNEEVLCIGLVERIGIEGSILKHE
                     KAGRDDKYVVEQPMKDHKDAIALVLEAVAHPEFGAVKEMKEIDAVGHRVVHAGEKFAT
                     SVVITPEVEEALKECIDLAPLHNPANIMGIDACKAILPDVPMVGVFDTAFHQTMPKSS
                     YLYGLPHELYTKYGVRRYGFHGTSHNYVSQRAAEILGKDIKDLKIVTCHLGNGASIAA
                     VDGGKCVDTSMGFTPLEGLIMGTRCGDIDPAILPFLMRKEGLDADGLDKLMNKESGVY
                     GMTGISSDFRDIEDAAKNGDERAQATLEAYVKKVQKYIGAYAAEMNGLDVVVFTAGVG
                     ENGKAIRADIASNMEFLGMKLDKEANDVRGKETVISTADSKVKMLLIPTNEELMIARD
                     TLRLVK"
     gene            53735..54259
                     /locus_tag="KR250_000167"
     CDS             53735..54259
                     /locus_tag="KR250_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HBH0066139.1"
                     /translation="MKVSIEKINRKETDKIDLNFCEKIDTISYCDEIYKLVSPVNLKG
                     KVSKTNKGLYLDIDVNFTIVDNCSRCLKEVEIPLEYSIQGFLVKEEDYDEDEFEEFDP
                     FIFDGEEIDLIDIIEQTLDFNVPHKVLCSENCKGLCQVCGANLNEEECSCSEITNDEE
                     YIDPRFAKLKDLFN"
     gene            54284..54460
                     /gene="rpmF"
                     /locus_tag="KR250_000168"
     CDS             54284..54460
                     /gene="rpmF"
                     /locus_tag="KR250_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HBH0066140.1"
                     /translation="MAVPKRKTSKSNTKMRRAANSKMEATGFVSCPQCHEPKLPHRVC
                     PDCGYYKGKEVVSK"
     gene            54667..55224
                     /gene="fapR"
                     /locus_tag="KR250_000169"
     CDS             54667..55224
                     /gene="fapR"
                     /locus_tag="KR250_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor FapR"
                     /protein_id="HBH0066141.1"
                     /translation="MKKKSKAQRQKELIDMLKTDPFYTDEELSSLFDVSIQTIRLDRM
                     SLNIPELRERVKSIAETQSSKVKTLGVKEITGEIIDLSVGRLGISMLEVTQDMIYSKT
                     NTLKDTYIFSLADSLAMAIIDAPKVIMRVANVKSFKLIEQQDRLIAKAEVYRNIDKKH
                     YVKVVINNKAQEQIFRGKFIFEELD"
     gene            55235..56257
                     /gene="plsX"
                     /locus_tag="KR250_000170"
     CDS             55235..56257
                     /gene="plsX"
                     /locus_tag="KR250_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HBH0066142.1"
                     /translation="MKIVIDGMGGDNAPKSNVEGAVNAIKEYQVDLIITGDKDLLEKE
                     FSNYEFDRNKLEIVHTTEIIENEDKPVKAIRSKKDSSMVVALNLVKEGKADAIISAGN
                     TGALLAGGLFVVGRIKGIDRPCLCSAIPNVKRGMTLIADCGANADCKPKNLVEFAAMS
                     NIYSRKVLGLENPKVALANVGLEEGKGNDLVKRSYEEIKKLDLNFIGNVEAREVINAY
                     TDIIICDGFTGNILLKSAEGVALSVMSLIKETFMASTKSKIGALLIKDDLRKLKSFID
                     YSEYGGAPLLGLNGGVIKAHGSSDAKAIKNAINQGIKFSKGKVVEDINQFISKYNEEN
                     KNNEDE"
     gene            56283..57269
                     /locus_tag="KR250_000171"
     CDS             56283..57269
                     /locus_tag="KR250_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HBH0066143.1"
                     /translation="MNTKAGILGVGSYLPEQSYDNFHFEKIMDTSDEWISTRTGIKER
                     RFAKESEATSDLASKAALKAIECAKLNVEDIELIILATITPDMSLPSTACIVQDAIGA
                     VNATAFDISAACSGFVYGVTIAKQFVETGCYKNVLVIGAETCSKFLNYDDRTTAVLFG
                     DGAGAAVIGPVNEGGILSTHMGSDGKGKDCLKVPAGGSRLKASKETVEANLHTIEMAG
                     SDVFKFAVRKMAETSLRALEKANLNTTDIDYLVPHQANIRIIQASSKRLELDMKKVYV
                     NIDKYGNMSAASIPVALDEAYREGKIKKGDNVVLVGFGGGLTWGASVVKWTL"
     gene            57378..58307
                     /gene="fabK"
                     /locus_tag="KR250_000172"
     CDS             57378..58307
                     /gene="fabK"
                     /locus_tag="KR250_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HBH0066144.1"
                     /translation="MNKICKILNIKYPVIQGGMAWVATASLASAVSNAGGLGIIAAGN
                     APKEAIKKEIVECKKLTDKPFGVNVMLMSPFVDDIIDLIIEEKVQVITTGAGNPAKYM
                     DRLKEAGTKVIPVVPTIALAQRMEKLGATAVIAEGTEGGGHIGELTTMVLVPQVADAV
                     NIPVIAAGGIVDGRGIAASFALGASAVQVGTRFICSEECSVHSNYKNLVLKAKDRDAI
                     VTGRSTGHPVRTLKNKLSKEFLKMEQNGATPEELDKKGTGALRFATVDGDIEKGSFMA
                     GQSAAMVKEITPCKEIIEAMVNQAREIMPAIEL"
     gene            58323..59273
                     /gene="fabD"
                     /locus_tag="KR250_000173"
     CDS             58323..59273
                     /gene="fabD"
                     /locus_tag="KR250_000173"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HBH0066145.1"
                     /translation="MGKVALVFPGQGAQYVGMAKDLYENNTVAKSVIDEASDALNMDL
                     KNLMFNGNEEELSKTENTQPAMVTHSVAVLKAVQAQVDLKYSACLGLSLGEYSALVAA
                     DAIDFKDAVCLVKKRGKFMQDTVPTGVGAMAAILGLDRSILENVVKDIQGGIVEVANY
                     NSPGQIVISGENEKIEEAMVKCKEAGAKRAVKLNVSGPFHSSMLREAGVKLASELEKV
                     NITKPKVDVVANVNADYYKNEGKDLLIKQVSSSVLWEDSIERLLNDGYDTFIEMGPGK
                     TLKAFIKKIASNKKANVNIYNIDGIDSLNEFVQNYRNGEI"
     gene            59273..60022
                     /gene="fabG"
                     /locus_tag="KR250_000174"
     CDS             59273..60022
                     /gene="fabG"
                     /locus_tag="KR250_000174"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HBH0066146.1"
                     /translation="MINLTGQVAVVTGGSRGIGKEIAKKLASFGADVVINYTSKEDEA
                     LKTKNEIESMGVKCTSIKCDVSKFDEVNQMIDSVVSEFGKIDILVNNAGITKDGLLMR
                     MKEEDFDRVIDINLKGVFNCTKAVTKPMMKKKYGRIINMTSVVGIMGNAGQTNYCASK
                     AGVIGFTKASARELASRNININAVAPGFIETDMTKVLSDDVKESTLANIPKKSYGKPE
                     DVANAVAFLVSDMSSYITGQVINVDGGMVMQ"
     gene            60076..60300
                     /gene="acpP"
                     /locus_tag="KR250_000175"
     CDS             60076..60300
                     /gene="acpP"
                     /locus_tag="KR250_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBH0066147.1"
                     /translation="MFNKVVEIIIEQLGVEDKEITMETSLMKDLEADSLDAVEIIMAL
                     EDEFGIEIPDTEAENFKSIGDIVKYIEANK"
     gene            60329..61567
                     /gene="fabF"
                     /locus_tag="KR250_000176"
     CDS             60329..61567
                     /gene="fabF"
                     /locus_tag="KR250_000176"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HBH0066148.1"
                     /translation="MNKRVVITGLGCVTPLGTGKEEFWSNIKSGVSGIDKITNFDAST
                     YQTQIAGEVKNFHPEEYISKKELKRLDKFAQFAIVSAKLAVEDANLDLDKVDRERFGV
                     IIGSGIGGVEAIETQHKILLEKGNKRVSSLFVPMMIGNMAAGQVSIFLGAKGPNTNVC
                     TACASGTHSIGDAFKVIQRGDADIMVAGGSEAAVTGLAFAGFCNMKAMSTRNDDPKTA
                     SRPFDKDRDGFVMGEGAGIVILEDLEHALARGAKIYAEVVGYGLTADAYHMTTPAENG
                     EGAARSMNMALKDGNVPLEEVDYINAHGTSTYYNDLYETMAIKTVFGEKAYDLCVSST
                     KSMTGHLLGASGAIEAVVCAMSIEDSFVPPTINIQEVGEDLDLDYVPNQGKEKNIRYA
                     LSNSLGFGGHNATIVLKKYV"
     gene            61793..63511
                     /locus_tag="KR250_000177"
     CDS             61793..63511
                     /locus_tag="KR250_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBH0066149.1"
                     /translation="MNFRLPKKRLPKKIIFICIILISIILFIKHDQNGLKEREIINKG
                     KYMSESLVADKEYDEAKKVVKTSFESIPEKKYNKVYNEVWDMLKTISYVPDGTKIVID
                     SLEKMRESDVKLSDECRFNIEKRLASVYIMDNNYFKAVDLTVKSIKLAEKLGNNYEKA
                     RLDVDLASIFLKIGGYETGEKLIKDSFKIDIDEGEKNGMVRLYAIINLAEIYVEEGEY
                     DKAIEISSRIKDYKKFVNTDNYNDFDIMASIMQSNAYVGKNNIQKAKTFLEKADYLIK
                     SDRTVYILDKDMYYYMAMGNLEYKSENYNSAIDNYKKSLEISTKRKLTQEKIKNLNKI
                     LNIYEKQGNIESLNEYQKMLINEYKENKSIRDSETSFYIIDKVSNESKLFEKTKKEIK
                     YYKILFTVILIAILGSTFLYNRLKYFKAQNLHDGLTNVYNRKSFDIMYEKYREKDDNF
                     ALVMIDIDNFKLINDNYGHKFGDTVIKGITNTICVMLEQDDKVFRYGGEEFSVLIRNK
                     SGDEVADIIDSIRVAIANKKWNDDVTVTISAGVAHVSDSKDVLEEADKNLYKAKQLGR
                     NKVVYK"
     gene            63542..65542
                     /locus_tag="KR250_000178"
     CDS             63542..65542
                     /locus_tag="KR250_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBH0066150.1"
                     /translation="MKQSIAIVTDKITKLSSFLEENIRLVLGNYIDINHYYLEVLKEN
                     DKIQGDFVLVMNDDRLNSMRKHLVQDSKIIVIRRTLKENEIYGLFSIPNGTEVLVVND
                     TKETTLETISLFYKIGVKNLKFTPYIEGKIYKNIDIAITPGVKGKVPSYINKVIDLGN
                     RYIDISTFIEIINGLRIDVKEIRKNLMAYSESLISLDNGVKDNYRQLFLKIEEQDVIL
                     NLSKDGIIFTSADGIINTFNKEALKILGINENPQGKNIEDIISEDLNILLEDKEIIDE
                     VVFTNKKYINVNKKSIFSMGNKAGIYYNLQEITYIKKLEQNLTNKLREQGQIARYTFK
                     DIKTKNKNMVKCVELAKKISKSELSVLIIGESGTGKELLSQSIHNASNRSNQPFIAVN
                     CAAVPDSLLESQLFGYEKGSFTGALREGKKGLFELANNGTIFLDEIGDMPPLLQTKLL
                     RVLQEKQVMPIGSHNVINIDVRVIAATNKNLLKMIREGKFREDLYYRLNVLPIDVPSL
                     RNRKEDIITLMNFFLKNNIRLSPEVITILEKYNWPGNIRELQNVASYVSLMCDSMVFR
                     DDLPPYIRVECANNYEDGFGYTRKEFEEILRILYERRESSSGIGRGFIIEKLIQEGIE
                     ISESKVRKVLSYLSECGYISSKSGRGGSQITSEGIKLYNNIN"
     gene            65893..66138
                     /locus_tag="KR250_000179"
     CDS             65893..66138
                     /locus_tag="KR250_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03905 family TSCPD domain-containing
                     protein"
                     /protein_id="HBH0066151.1"
                     /translation="MKYKTTGTCATEIEFEVKENKVTNVNFIGGCDGNLKGLKVLVEG
                     MNIEDVIQKLKGIECKTKPTSCPDQLSLALENYMNIK"
     gene            66173..66898
                     /locus_tag="KR250_000180"
     CDS             66173..66898
                     /locus_tag="KR250_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HBH0066152.1"
                     /translation="MKPIIGILGNLIIMENGMFPGLERSYVNNDYINAVLKGGGSPVI
                     IPVNTDKEVIKKQIEMVDGVLISGGWDINPQLYGEETREETTFIYPEVDEFDLIAISI
                     ALELKKPILGVCRGLQILNVSLGGTLYQDNNLIEGSYIKHTQSSKRHVATHKVDVKEG
                     SILEGILGKQLLTNSYHHQSVNQLGKGLKAIAYSKDGIIEAIEKEDENFVVGVQWHPE
                     MMVDYCDKMEKLFKYFINICSNL"
     gene            66920..68233
                     /locus_tag="KR250_000181"
     CDS             66920..68233
                     /locus_tag="KR250_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBH0066153.1"
                     /translation="MNEKNKMGLISIILLGINAVVGAGVFLLPGDAMKSFGVASIFVY
                     IFDMLLVLSMAFCFAEVAGKFNKNGAAYVYTKEAFGDFCGFEVGLMKWVIGCISWGAL
                     IVGFPTSLSAVWAPAGEPHIQKIIIVVMIVGLTIINLLGVSLSKIVQNVITVGKLIPL
                     ILFIGIGIFFIKGVNFTSSTMVPPGAGATEFGAAALLMFYSFTGFESIAVAAEDMENP
                     QKNIPIAIISVIVIASIIYILNQVVCVGILGDSLSSTSTPVADAARICFGNMGAGLVT
                     FGTLVSVGGICMCGAFVNPRSCVALADDKMLPRIFARKDKKGTPYVAIIATMLITIPI
                     ALSGSFAELAAISAVARFIQYIPTSLSVLVFRKKRPELVGTFKTPFGAVIPLIAVCVG
                     TWLLFQASMHQLIMGLGALAIGVPLYFIMKSYNRKVYDENLKKIV"
     gene            68439..68978
                     /locus_tag="KR250_000182"
     CDS             68439..68978
                     /locus_tag="KR250_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBH0066154.1"
                     /translation="MPTITLKNGVDVLIREGVREDAQSIIDFYNEVGGETHFLSFGKD
                     EYKISLEEQENAIESAKASDNSVKLIAFIDGEIVGIATIDSNQKAKGKHVGVLGIVVK
                     EKYWGIGLGKRLMLDLIEWCKSNGITKKITLVTNEENYNAIGLYKKVGFEVESILKKE
                     CYYNGVYTDLIGMSLLLGI"
     gene            69067..71052
                     /locus_tag="KR250_000183"
     CDS             69067..71052
                     /locus_tag="KR250_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1,6-bisphosphatase"
                     /protein_id="HBH0066155.1"
                     /translation="MKNLCKISDDYLNSKLKYLKLLSKQYPSISKASTEIINLEAILN
                     LPKGTEHFITDVHGEYEPFVHVLKNGSGVIKRKIEELFSNTIRDSEKKMLATLVYYPE
                     QKLDLIIKQEENIDDFYRINIYRLIELCKYASSKYTRSKVRKLLPENFKYIIEELLHE
                     HVKSEHKEEYYKSIVETIVDIGIAKEFIIAISTVIQKLVVDRLHVIGDIYDRGPRPDI
                     IVDKLIEHHCVDIQWGNHDILWMGAASGEKTCIANALRISARYANLDIVEDIYGINLL
                     PLATFAIEMYKDDPCKEFIPKVNDQSVTTTEKSLMAKMHKAISIIQFKLEGEVIRRRP
                     EFEMEHRLLLNMINYDEGTINLKGKTYKLKDTYLPTIDKKDPYKLTMEERNVIDKLVS
                     SFRGSEKLQKHVSFLFSKGSIYLKANSNLLIHGCVPLNEDGSFMSMNIMGKEYKGKAL
                     MDKMESLAREGFFFKDKAEEKLYGMDIMWYLWTGKCSSLFGKDDMTTFERYFIAEKET
                     HKENKNPYFKLRENEMACKRLFEEFDLELDESHIINGHVPVESKNGESPIKANGKILV
                     IDGGFSRAYQKTTGIAGYTLIYNSRTLQLVSHEPFNSAEEAIANESDILSTTVVVEHK
                     AKRKMVRDTDEGVKIQEEIEDLKLLLMAYKKGLIKEM"
     gene            71276..72244
                     /gene="spoIIIAA"
                     /locus_tag="KR250_000184"
     CDS             71276..72244
                     /gene="spoIIIAA"
                     /locus_tag="KR250_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AA"
                     /protein_id="HBH0066156.1"
                     /translation="MNKLSDEIINSLSTTIREKVEKVSNNNLNIEEIRLRSQKPLILN
                     ANSKDYFYNQKTMTLDLNQQNSYVVTREDVEQTFQIICKYSIHSFMDDIKKGFITLRG
                     GHRVGLVGKAIVEDGQVKNIKHISSLNIRVSREIIGCSDKILSHIIKGKNQINNTLII
                     SPPQCGKTTLIRDIVRNLSNGNEDYGFKGLKVALVDERNEIAGAYLGVPQMDVGIRTD
                     IIETCPKDLGITMLLRSMSPNVIVTDEIGSEKEIKALYTALNGGIGLITTVHGDSIED
                     IQNRKELNRLLDKELFKKVIILSAKRGAGTIEKIYDLEEKRWYFAN"
     gene            72234..72755
                     /locus_tag="KR250_000185"
     CDS             72234..72755
                     /locus_tag="KR250_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AB"
                     /protein_id="HBH0066157.1"
                     /translation="MQIKIIIIAFLIGSSYLIGEQIYKTYTRRHKQLNDLIRVLEILR
                     MDLSFGLYTLEEIFNRIGGNKEFCFWKFFYQISEGLHNEQSKTLEIIISENIDVLSKE
                     TYLGNKEIEELKNLILTLGKSDIESQQRMIDLSIENLKKQTYETKEDINKKGVLYKKL
                     VTFIGIGICIILI"
     gene            72772..72969
                     /gene="spoIIIAC"
                     /locus_tag="KR250_000186"
     CDS             72772..72969
                     /gene="spoIIIAC"
                     /locus_tag="KR250_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018589775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AC"
                     /protein_id="HBH0066158.1"
                     /translation="MEISLILKVAGVGILISVLNMILEKTDRKDWAGLTTLAGVIIVL
                     GMVITEISDLFNTVRTMFQLY"
     gene            72985..73371
                     /gene="spoIIIAD"
                     /locus_tag="KR250_000187"
     CDS             72985..73371
                     /gene="spoIIIAD"
                     /locus_tag="KR250_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AD"
                     /protein_id="HBH0066159.1"
                     /translation="MEIMQLIGIAIISTTLCLVIKKDRPEIANFIAIITGVIILLSVM
                     FKLNFIVDSIQDLANKANIPTMYISLIIKLIGIAYLMEFAIQLCKDCGEGNIASKLEF
                     GGKIIVMSMSFPILLSIVEMVVNIIP"
     gene            73456..74661
                     /gene="spoIIIAE"
                     /locus_tag="KR250_000188"
     CDS             73456..74661
                     /gene="spoIIIAE"
                     /locus_tag="KR250_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AE"
                     /protein_id="HBH0066160.1"
                     /translation="MKKRFLPMIIGFLFTFFFVNIFAIMIFANEVPSSEDKEYGETKN
                     SIDKYIDGQLDKLDINEIQDYINKEIVINDVNLKSFVKDLISGEKNILDLFNKDGLKI
                     LMFDEFKASLKVVAVILVLALLSSILKSLENSFSSGAVSQIATYIIFITMVSLTLIGF
                     KDVLQICYDAIDHTVGLMQVIMPILITFLLLIGFPITSTTLNPIFIGGVTFINVFFKN
                     FLFVSITVAFGILIINNLSKNIRLKRFFSFVKQINYVSIGAMFTVYLGLVSIQGLYVT
                     SFDKFSVKTAKFAIGNFIPVVGGFVSDSVDILLSSSQLIKNIFGGIGLILLVGICLLP
                     VIKILSVIVVYKLAAIIVEPVGEDGISNFLNEVANLMIIMLASVIAITVMFFVTVAIL
                     TSISVVSQG"
     gene            74721..75338
                     /locus_tag="KR250_000189"
     CDS             74721..75338
                     /locus_tag="KR250_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AF"
                     /protein_id="HBH0066161.1"
                     /translation="MLEGIKAWIVSVLIGAFIVNIVDMILPSSKIKPYVNLVLNFMFV
                     FIVITPVVGFFSKDMSLEDRILKSMGNYNKQYVDSTNALAKETGNNSLSKGYEDGLKE
                     VLKLKLDEYGYDLEDIELNGANINNIKIKEKNNSAKSNNSSNEENKNNSTKREEENIN
                     SNDKENSKQVFKKGTEYGLNLNEEKLKNDLIKVLDVSIEDIQIDK"
     gene            75350..75904
                     /locus_tag="KR250_000190"
     CDS             75350..75904
                     /locus_tag="KR250_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AG"
                     /protein_id="HBH0066162.1"
                     /translation="MLKNLNDKDKRKVYSLITIAGICVVSLVLLSCFPSSKSDKQVGK
                     TETNKNAEKQVTKEQEKDDLESKLTAILSKIDGAGDVDVMVTFESSEEIQPAFNSNNT
                     TETTEEKDAQGGERTVTTSSENKTMITSNSSDPVVIKTTEPKIKGVIVVASGASDPSV
                     KETLYSAVQTSLQVAGHQVEIYSK"
     gene            75931..76620
                     /locus_tag="KR250_000191"
     CDS             75931..76620
                     /locus_tag="KR250_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIIAH-like family protein"
                     /protein_id="HBH0066163.1"
                     /translation="MKFNYKGRGFVIITLTAMLVVVGTVNYQLSKKSLLETSKEFKAY
                     EEAQLQKNTDDSYSSKTEDSSNVDKQGGKESADIDIVDSKASKVKEKATETSKEIKAQ
                     LSSEKNMKKASYILDMKMNREKQRNELVQDLNEMINNPSTTEESRKEASNMKLNIVKI
                     QEKELQIENLLSTKGYEEALVYISDNKVNVVVNEAKLEKKDAAKIFDLVAEQANVKYE
                     NIKLTNNNSNK"
     gene            76730..77092
                     /locus_tag="KR250_000192"
     CDS             76730..77092
                     /locus_tag="KR250_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBH0066164.1"
                     /translation="MEDNKFGQVKISNDVIATIAGLAALEVEGIETTATLTDKLLKNN
                     GVKIQIEEEDVNLDVMVTIKYGMSIPDTAFKVQENVKNTVETMTGLKVSQVNIHIQGI
                     SFKKDKVDKEEAKATKKN"
     gene            77177..77683
                     /gene="nusB"
                     /locus_tag="KR250_000193"
     CDS             77177..77683
                     /gene="nusB"
                     /locus_tag="KR250_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HBH0066165.1"
                     /translation="MKKDRAQKSTTREYIMKLIYQININKEDFETLEDKVDNFLKDNS
                     EHIINRYKELALQYSKNTNLKLEDTEIEDVIDKKYINTVCKALKENHDKIDELINKHA
                     KNWTVDRMPKVDVSILRLSVCEILYLDTPNKVSINEAVELAKIYCDDKSPKFINGILG
                     SVVDEIGK"
     gene            77667..78737
                     /locus_tag="KR250_000194"
     CDS             77667..78737
                     /locus_tag="KR250_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-sialoglycoprotein endopeptidase"
                     /protein_id="HBH0066166.1"
                     /translation="MKLGNNIIIGIDTSCYTTSIAAISLDKKVIFNEKIMLEVRDNSK
                     GLRQSEAVFQHINNLGILSDRIKSFKDKFNVEGVCSSKKPRPVENSYMPVFNVGHNFG
                     KLLSSIYGCRFYETTHQENHIEASLLNSKLKNNNKFISVHMSGGTTEILLTSKQDSHH
                     NVCDTNLGKIAKISIKKDDKSKLYNNFGYNIDIIGGSKDISFGQLIDRVGIKLGYKFP
                     SGKYLDENALNCNLKIESGLKTSVRDGYMNLSGLENQVNKIINDNGDNTNQKEYISKL
                     VLDSVVRNMFKSLVYLCETYNVNEVVFAGGVSASKYILRELSMKLRKKHIEAYFTEPQ
                     YSTDNAVGCAIIGLNNFLGERV"
     gene            78734..79939
                     /locus_tag="KR250_000195"
     CDS             78734..79939
                     /locus_tag="KR250_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HBH0066167.1"
                     /translation="MKLRALDISEANSYIKRILINDPILSNLKVKGEISNFKVHSSGN
                     VYLSLKDETSKLNCVIFKSNFNRNLKLDNGVKIIANGYISVYERDGAYQLYINEIEIE
                     GIGNLHIEFNRLKEKLNKEGLFDPKYKIPIPKMPNSIGVITSPTGAVIRDIINVIKRR
                     YPKVNIKLYPVTVQGDKSAEEICEAIRFFNHMKNVDTLIVGRGGGSIEELWSFNDEMV
                     AREVFNSQIPIISAVGHETDFTICDFVSDMRAPTPSAAAEIATPSLDDINYKLGNIKS
                     RMSKSLTNQIELDQYRLETVFNKINNYLDSYTIKDKVIQLDKIYDKIIFGIENNLKLE
                     DEKLVKIGALLHNLSPLATMDRGYSITQKNGKVINSIKGLKIKDSIDIVLKDGNLECM
                     IDKIENKEG"
     gene            79941..80156
                     /gene="xseB"
                     /locus_tag="KR250_000196"
     CDS             79941..80156
                     /gene="xseB"
                     /locus_tag="KR250_000196"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HBH0066168.1"
                     /translation="MNLTYEEAYKRLESILSELESKNASLDESLSLYEEGISLYKHCN
                     KLLDDAKLKISKFNQLGIEEDFKIEEE"
     gene            80159..81046
                     /locus_tag="KR250_000197"
     CDS             80159..81046
                     /locus_tag="KR250_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HBH0066169.1"
                     /translation="MEFKQCLKEKASFVEKVLKEYMPKEEGYQKTVIEAMNYSLSAGG
                     KRLRPILTLEACKIVGGNEDEAIPFAIAIEMIHTYSLIHDDLPALDNDDLRRGRPTNH
                     KVYGEAMGILAGDALLNYAFEVMLAGSINKENPEKYLKAINEIAKGAGIYGMIGGQVV
                     DVESENKQIEKEKLDYIHMNKTAAMMVGCMRAGATIGGANSEQMEEITKYAKNIGLSF
                     QIVDDILDIVGDEAKLGKKVGSDIENHKSTYPSLLGLDKSKEIAHNLIDEAKKSIEKL
                     SDDVDFLKGLAEYIIDREY"
     gene            81076..81513
                     /locus_tag="KR250_000198"
     CDS             81076..81513
                     /locus_tag="KR250_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent PAP2 family protein"
                     /protein_id="HBH0066170.1"
                     /translation="MDFFSEIFNNGALGISLIACFLAQFIKIFTGKEKRIELSRILIS
                     GGMPSSHSSFVTSLATVVGIEKGFNSTDFAIITVLALIIMYDAAGVRRAVGKQATILN
                     QMVADIQHGKHIEQKKLKELIGHTPLEVWFGALLGIVTALILM"
     gene            81547..83412
                     /locus_tag="KR250_000199"
     CDS             81547..83412
                     /locus_tag="KR250_000199"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HBH0066171.1"
                     /translation="MYKYLDKVNSPKDIKNMSIEEMDLLAKDIRKFLVKSVSKTGGHL
                     ASNLGVVELTLALHKVFDSPKDKIVWDVGHQSYVHKIVTGRKDCFVSLRQFNGLSGFP
                     KENESPHDIFDTGHSSTSISIATGIACARDIKKENYSVISVIGDGSITGGMALEALNQ
                     LGYIDTNMIVILNDNEMSIDKNVGGMSKYLSSIIRNSTVEKMTDEVDKILNVTQTGEI
                     LSKTAHRFKDKLMYSFSPQDCSFFDSLGIRYYGPIDGHNTKELIDILRKAKHKKGPVL
                     LHVITKKGKGYRFAEEQPDKYHGVSKFDIKTGVTSAKVKSMSISVGEKLVDMANSNEN
                     IVAITAAMPSGTGLNLFESAYPKRYYDVGIAEQHATGFAAGLAKNGMKPYFAVYSSFL
                     QRAYDQVIHDVCITKKPVTFLIDRAGLVGNDGETHHGMFDLSYLNSIPNIVVMAPKDT
                     REMELMMDLSLKLDCPLAIRYPRGSSYYLDKGEYGEIVLGKYEVLDDGQDTVILCIGS
                     MVKHALEAKEILSREGINPTIVNARFLKPIDEGMLKALLKNHKNVVTIEDNIVTGGFG
                     SRINKFIIDNEYNVNILNIAIPEEFVKHGNIDELYDFVGLSPKSIADKIRKLVIE"
     gene            83545..84357
                     /locus_tag="KR250_000200"
     CDS             83545..84357
                     /locus_tag="KR250_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family rRNA
                     (cytidine-2'-O)-methyltransferase"
                     /protein_id="HBH0066172.1"
                     /translation="MKKRIDLLLVEQGYFESRERAKKAIMAGLVFVDNQRCDKAGTEV
                     KEDCSIEVKGNPIPYVSRGGLKLEKAMKNFDLTIDGKVCMDIGASTGGFTDCMLKNGA
                     IKVFSIDVGYGQLAWKLRQDDRVVCMERTNIRNVTIEDTKQFADFASIDVSFISLKLV
                     LPKAKELVRHDGEVVALIKPQFEAGREKVGKKGVVREKSTHIEVIKMISDFSVENGFE
                     ILGLDFSPIKGPEGNIEYLIHLRNGNEGYEFDGETYNNKIVEVVEASHNLDK"
     gene            84377..86056
                     /gene="recN"
                     /locus_tag="KR250_000201"
     CDS             84377..86056
                     /gene="recN"
                     /locus_tag="KR250_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HBH0066173.1"
                     /translation="MILELYMKNCALVEELRLNIDKNLNILTGETGSGKSIIIDALGL
                     CLGDKYDRSFLRKGTDKGLVEAVFFSDNRYLKKILEENDISMEDDNLLVITRLIYSDG
                     KSTARVNGRTVKVSLLKEIASTLIDIHGQHQNQALFNKDTHLKFLDLFGENELEEFKI
                     AYKNVYHKYSEVKKALNCLTENKDEMQIQREIDLLRFQINEIEAANLNKNEYEDLLKQ
                     REVYRNSEKIYNNLNSSYSKLHNGEYNVIDLIGLASKELNDISKYDSVLSEYSDTVER
                     IMYELQDISGEIRNYKDNIDFEPYELEQIELRIDEINNLRRKYGDSIDDIFEYYGKIK
                     DRLDEILNRDERVEQLRSKLMNIEEDLKIKASKLTKARIEVATTLEKILLDELKSLNM
                     KNVMFKVNFEKGPFTLDGVDDIEFMISFNLGEDIKPIYKVASGGEMSRFMLAFKTILA
                     DIDDIDTLVFDEIDTGISGIAAQIVGEKLSDIAKKKQIICITHLPQIAANADTHYCIE
                     KDTSNNRTFTNVSKLNESQRKNEIARLIAGNNITEKTIEHASEIIELAKKC"
     gene            86271..87137
                     /locus_tag="KR250_000202"
     CDS             86271..87137
                     /locus_tag="KR250_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0066174.1"
                     /translation="MNKKDTMHLIFIIVIFILLWYIVVAVSEINKNIESCSNKLDYLS
                     QELMSTKSDISNTINEEMSKSYITKSIDLKVKKIEKKECIIDIDVQLSKLGKNGKVFF
                     MYKEDSDKWNETEMTKHGELSYACEIKINTGSEYDYKVVTSGAISESSDVQKLTKSDY
                     MPEQPIFNSGIRDNREYFIEIVENSNLFNHESEKSKNKVYDNIRLEKVDIIVNEGKKD
                     TIYKAKLAEGLDDGKTNNSNRNQVNYEVSFPKRDIDDIIYIKAKLTYNNGVEYTKDIT
                     EDITMGLQDLEK"
     gene            complement(87230..87721)
                     /locus_tag="KR250_000203"
     CDS             complement(87230..87721)
                     /locus_tag="KR250_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBH0066175.1"
                     /translation="MADKMAQNITLQFVEEKDLENLENVKLLFTEYSNSLNIDLCFQD
                     FNNELKTLPGKYKKPSGSLILAFVDENLAGCVALKKLEGKICELKRLYVRNQFRGLKI
                     GKILLEEIIEEAKKFGYTHMRLDTLPSMKSAQGLYEKFGFYDIEPYTYNPIEGARYME
                     LKL"
     gene            complement(87782..88222)
                     /locus_tag="KR250_000204"
     CDS             complement(87782..88222)
                     /locus_tag="KR250_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBH0066176.1"
                     /translation="MKLNVRIADINNWHDLVSLSVDKSQLDYIESNALSIAESKFITA
                     WVPVGIYDENSLIGFAMYGRLEDDRVWLDRFMIDSKYQGKGYGKASLDFLVNHLKNEY
                     NCDELYLSIFEDNKMAIKLYKDFGFEFNGELDYGGEKVMVLKSN"
     gene            88383..89441
                     /locus_tag="KR250_000205"
     CDS             88383..89441
                     /locus_tag="KR250_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S55"
                     /protein_id="HBH0066177.1"
                     /translation="MNKINKNFKNIWIILGAVSIFTMVIIFNKNCNVNKETQIEAFTI
                     KNHDKKFVYPMGNIIGVKANTDGVLVLGYEEEDVDYIGGIQIGDNIVKIDNKRIKNSQ
                     DVSEILNKIKKSKVEVTFERKNKYKTEVIETKKENGKYKLGLWVRDKISGIGTMTFYD
                     PSMEKFKAIGHAIKDSDTNELLKIKQGYIYKPEQLKIVKASNEKVGKIKGDFNDSNLM
                     GNFSNNSELGISGNITENHNKEFNVANKEKQLIEVGRPQDVKIGGAVILFEDKNKNIT
                     SYDIKIESIVYDKGNYRDMVIKVVDDKLLEYTGGIVQGMSGAPIIQNNKIIGAITHVF
                     RDNPKKGYGIFIDEMIKL"
     gene            89583..90407
                     /gene="spo0A"
                     /locus_tag="KR250_000206"
     CDS             89583..90407
                     /gene="spo0A"
                     /locus_tag="KR250_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcription factor Spo0A"
                     /protein_id="HBH0066178.1"
                     /translation="MGGFLVEKIKIVLADDNKDFCQVLKEYLSNEDDIDILGIAKDGI
                     EALDLVKKTQPDLLILDVIMPHLDGLGVIEKLNTMDIPKMPKIIVLSAVGQDKITQSA
                     INLGADYYIVKPFDFVVFINRIRELVSNRVTQVEPKPRPVQETQMTRSDFVKNVGNIE
                     TEITNIIHEIGVPAHIKGYLYLREAIKMVIDNVELLGAVTKELYPSIAKKFNTTPSRV
                     ERAIRHAIEVAWSRGKVDTINQLFGYTVHNTKGKPTNSEFIAMIADKLRLEHSMVK"
     gene            90820..91941
                     /locus_tag="KR250_000207"
     CDS             90820..91941
                     /locus_tag="KR250_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0066179.1"
                     /translation="MRVEAPIKVDRKTKKLAKRVESGEIAVINHIDIDEVAANSLVEA
                     KIKLVINAAPSISGRYPNKGPGILTENNILIIDNVGEELFEELKEGETIEVVDGKIYR
                     KGKFLGAGEVLDKYEVSHQIKAAYENLAVELDRFIDNTIDYAKKEKGFILGEVEIPKV
                     KTNYANKHVLIVVRGQDYKEDLSTMLSYIEEVKPVLVGVDGGADALIEFGYTPDVIVG
                     DMDSVSDEALKKASEIVVHAYTDGRAPGLKRVEELGLDAVVFPAPGTSEDIAMLIAYE
                     YKAELIVAVGTHSNMIDFLEKGRKGMASTFLVRLKIGSKLIDAKGVNLLYRSKLKIKY
                     IWALIATALFPVLVVASLSPGVQQFIQLMQLKFRVLLQM"
     gene            91959..92834
                     /locus_tag="KR250_000208"
     CDS             91959..92834
                     /locus_tag="KR250_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper transporter"
                     /protein_id="HBH0066180.1"
                     /translation="MHINMKYYIVTIGAIFIALGIGMLVGFNLNNNQQLSEQQANIIN
                     DLDDKFDILKEKNDKLDSDLASVNRDYEEAVNFINKNVDKILAGSLNGKSIGIISANE
                     NDDYTKNIEDIINKSNGSIAFNIILKENITNPEKLKEMSTKLGTDVKNANDAVNYIID
                     TLKKEDASDILTYLQELDVIKFNFIGDTYLKYDSVVIAGGNDAKDSTKQFEKIEKFVV
                     SKLKSENKYLVEVQNTGVKTSYVELYSKNKVATIDNIDEGIGSISLAILLQQGNIVGN
                     FGRLDTATSLLPSIK"
     gene            92858..93550
                     /locus_tag="KR250_000209"
     CDS             92858..93550
                     /locus_tag="KR250_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBH0066181.1"
                     /translation="MDSYISIIIPAYNEESRIKYTLESILDIKQINEIIVVDDGSIDN
                     TAKVVSELENEKIKFFKLDKNRGKGYALNYGIKLAMKNADIIGFLDGDLGSSAKEVEK
                     LIVPILNGEADVTIAKFPPAKRKGGLGFVKGLAKNSVFEMTGVELDATLSGQRIFKRE
                     VLEKFDEIPFGYGVEVGMTIDILKYGFKIKEVLVNMTHNETGRNLKGFIHRGKQYYHI
                     KKVLGQKKKEWR"
     gene            93558..94382
                     /locus_tag="KR250_000210"
     CDS             93558..94382
                     /locus_tag="KR250_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HBH0066182.1"
                     /translation="MKDYILYTILLLTGLFGTYAVIPLFKNLLINGNVLRPNYKKDMI
                     PVSMGIVFLPMIVINGIILAFFTTEFKDLAYIFMFMFGMISMFFAGILDDVIGNRDVS
                     GLKGHFKSLFKGSLTTGGFKALFGGFVGLIVSVAISKDIIDIIVNTLIIALSTNLMNL
                     LDLRPGRAIKAYLFIMVIIFLTLTGFVQVLPLLIVPNVLAYFNYDLKARAMMGDTGSN
                     VLGISIGMLISFGYPFNIRLGWLIFVVIMHIFTEKYSLTKIIEKNKLLNFIDKLGR"
     gene            94387..95454
                     /locus_tag="KR250_000211"
     CDS             94387..95454
                     /locus_tag="KR250_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3866 family protein"
                     /protein_id="HBH0066183.1"
                     /translation="MISKRVGIVESIVSQTETLDDIRVNINGEIQRAYNYPKISGTIN
                     IGDEVVLNTTAVELSLGTGGYHFVITNLNNIESTLTEGGHIMKLRYTPLQIKVDSVEE
                     QESIYHDKFANFKGLEGLSVVVGTLHSMLTPFAASFKRNNPNKKLVYIMTDGASLPIY
                     LSKNVDTLKEKKLIDSTITIGNAFGGDYECINIYTALITAKEILKADAVFVSMGPGIA
                     GTGTKYGFTGIEQGSILDAVKKLEGRAIAIPRISFADKRERHQGISHHSMTVFKEIVN
                     VNVDIPITIYDDEKLNFIKEQINLNGLDKKHNIIYIDNNKTKEDLDYFNLKVKSMGRN
                     YEQDEAFFKAASTAAYYLMEV"
     gene            95456..95992
                     /locus_tag="KR250_000212"
     CDS             95456..95992
                     /locus_tag="KR250_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBH0066184.1"
                     /translation="MVLEEKTISSDRVYTGKVITLKVDTVEIPGQGYQKRELVEVGGA
                     VGIVAITDDNKVVLVKQFRKPIEKPIFEIPAGKLEKNESPKECAERELKEETGYSAKN
                     IKLIHKFFTSAGFSNEIMFVYLATGLTPGENNLDADEFLDVYEIELEEAYNMVLKNDV
                     EDAKTSIGLLLVKDMFKN"
     gene            96060..96665
                     /locus_tag="KR250_000213"
     CDS             96060..96665
                     /locus_tag="KR250_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HBH0066185.1"
                     /translation="MRRTYRKNDFRELNRQIIIIGLLFMMSIVVGSYINKILPGSSNN
                     ILNNINPAVEYYNLNISIKDTVIQNLKSDAIFMGSIALLSLFVVTIPAVLVAFVLKGM
                     SIGYTINSCILALKFKSIKMILIILFKNLIIIPCAIILALISLSYFKEMVYEFKKKNR
                     KNMQFLIKRYILNIIIIIALSLGLQLILNTASIGIIKFLAK"
     gene            96677..97591
                     /locus_tag="KR250_000214"
     CDS             96677..97591
                     /locus_tag="KR250_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBH0066186.1"
                     /translation="MDIIEGYIDYIKDKKKLSENTVASYFMDIKKYMEYINQKSIKLV
                     DIVENDIISYLIKLEKDNVSIATIARMISSIKSFHDYLFLNHICTNNPAKDIKKPKIK
                     KENINILTEEEIERLLNFPKLTTPKLIRDKAIFEVLYGTGIKVSELVEMNIEDIDLDI
                     DYIYCNSCCKNQRVIPLCDITKLYLEKYLKEARPKMAVEGEKSLFVSSLGQRFTRQGL
                     WKVIKKYSNLANIDKNINPTMLRHSFAIHLLNEGANIAVVSKILGNVNLSSLQVYLNH
                     IDKNVRREIKEKHPRNDVDVELQKAEAK"
     gene            97633..98805
                     /locus_tag="KR250_000215"
     CDS             97633..98805
                     /locus_tag="KR250_000215"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HBH0066187.1"
                     /translation="MSRVIWIVIDSVGIGALPDAEKFGDSKDVSTLGNIFKEYPDIQI
                     PNMRNLGIGNIDGIDFFESIKEPIGCFGKCKEMSQGKDTTTGHWEMTGIIVDKPFKTF
                     EHGFSKEIIEEFEKKTGRKVVGNKPASGTVIIDEYGEHQIKTGDVIVYTSADSVFQIA
                     ANEEVIPLEELYNMCKIAREIMMGDNAVARVIARPFIGKKKGEFVRTSNRRDYSLDPF
                     EPTVLDNIKESGLDVLAVGKIEDIFNGKGITDAIHTKSNMDGVDETLNYMKQDNKGLI
                     YSNLVDFDSKYGHRRDPEGYKKALEEFDSRLPEIMANMREDDILIINADHGNDPTYKG
                     TDHTREYIPVMIYGNKIKKGFNLGVKDTFADIGATVADILNVKLPKHGSSFKEDLF"
     gene            98852..99664
                     /locus_tag="KR250_000216"
     CDS             98852..99664
                     /locus_tag="KR250_000216"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HBH0066188.1"
                     /translation="MFEKIAQSSKFINSKSDIKPKIGLILGSGLGDLANDIEDPVAIK
                     YSEIPNFPVSTVAGHAGQLVIGKLEGKEVIAMQGRFHYYEGYSQKEATFPVRVMKELG
                     VEILIVTNAAGGVNKEFKAGDLMLIRDHINFSGSNPLVGKNDDRFGARFPDMSDAYSS
                     KYVDVVKNCAKECNIDVKEGVYMFFSGPNYETPAEVRMAQILGADAVGMSTVPEVIVA
                     SHSNIGVIGISCITNMAAGILNQPLSHEEVIETTQKVKSEFMNLVKTTVKNL"
     gene            99868..101193
                     /locus_tag="KR250_000217"
     CDS             99868..101193
                     /locus_tag="KR250_000217"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HBH0066189.1"
                     /translation="MRMYDIIKKKRDNLELSKKEINYFIENYSKGDIPDYQASALLMS
                     IYLNKMNKQETVHLTEAMMNSGDMINLSEIDGIKVDKHSTGGVGDKTTIALIPLVASC
                     GAPVAKMSGRGLGHTGGTIDKLESIPGFSTEMEISKFIDSVNKSKIAVCGQSAKVAIA
                     DKKIYALRDVTATVDNISLISSSVMSKKLASGADAIVLDVKTGDGAFMKTLEESFELA
                     KSMVDIGTGMNRDTIGIVTDMDEPLGFAVGNSLEVIEAIETLKGNGPKDLVELCEVLG
                     AYMLVLSKVAHDFEDGVEKIRESIKSGTAIQKLKEFIENQGGNKDVVDDYSLFKKSKY
                     KYPVKSTKSGYISKIKAEDVGVSAMILGAGRETKDDILDLSAGIILEKKVGDYVNEGE
                     VLAYMYYNDEQKLSLAKGRFIDSYSIVDSKIEKNKLIYGIVTKEEIKKF"
     gene            101406..102485
                     /gene="mltG"
                     /locus_tag="KR250_000218"
     CDS             101406..102485
                     /gene="mltG"
                     /locus_tag="KR250_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HBH0066190.1"
                     /translation="MNFKENRLKIAVLIIVILIILAGIFVFIQIGPYDKNNKKDVIID
                     VPSGASVGKISDILYENKLIKNELLFKLLVKVSNKAPSIKSGTYLLNQSYSNNDIISL
                     LVSGKIYQDGIKVTIPEGATSKEIIAMLVSKNLGDKATFENLIKKPQEFYDKFPYLKE
                     DGITSLEGFLYPETYYFNSKKQSEEDILSEMLKVFDSKYTDKFKKKQKELNMTLQEVM
                     EMASIIEKEAVLDKDRPIIASVFYNRLKVGMPLQSDATIQYIFEERKKIVTYDDLKID
                     SPYNSYKNKGLPPTPISNPGIKSIEAALYPEKTDYLYFVAKIDGGNNYSTNYQDHLKY
                     VKEYKEARDKQSKDTKATNKENTKK"
     gene            102657..103331
                     /locus_tag="KR250_000219"
     CDS             102657..103331
                     /locus_tag="KR250_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HBH0066191.1"
                     /translation="MSNIVNDLVEDYIRVTLKEKEGFLKDLEIYAEENSVPIIHKEVS
                     DLLKVLLKVQKPKRILEVGCAIGYSSIFFATIIGKDADIITVERNEKMIEKAKENIKL
                     AGFDNNITILEGDAEEKLSQVQGEFDLIFIDAAKGQYKLFFDLVIDKLKDGGLLVSDN
                     ILYKGMVAHDDFVVRRKKTIVKRMRNYLDYICNCDYLSTSLVPIGDGVALSYKESKRG
                     DVDGIK"
     gene            103318..104565
                     /locus_tag="KR250_000220"
     CDS             103318..104565
                     /locus_tag="KR250_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBH0066192.1"
                     /translation="MELNKVELLAPAGDLERLKIAITYGADAVYIGGEIFGMRSAAKN
                     FSKEDMAEGVAFAHERGKKVFVTVNIIPHNEDFLQLEDYLLELEEIGIDAVIIADPGV
                     LSVIKKVIPNMEIHLSTQANTTNYLSANFWYEHGIKRVVVARELSFDEISEIRAKTPL
                     DMDIEAFMHGAMCISYSGRCLISNYMTGRDANKGSCAQSCRWQYHLVEEKRPGEYFPI
                     YEDERGTFFFNSKDLCMIEYIPELIKSGITSLKIEGRMKTAYYVATVVRAYRMAIDEF
                     YRDPENWKFNPMWMEELKKGSHRHFTSGFYLNKPTTEDQNYQSASYVRNYDFIGIVRE
                     TEDEDGLIVVEQRNKMCVGDEIEVMGPYKETMFTKIEEMYNEEGEAIESAPHPRQIVK
                     LKLSVKVGKDYMLRKVIEEKVEE"
     gene            104637..106301
                     /locus_tag="KR250_000221"
     CDS             104637..106301
                     /locus_tag="KR250_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HBH0066193.1"
                     /translation="MSKKKTPFLKKVGKRSWCIFTIILIIYSVLIYRLVDIQVLKGDK
                     YKQSVESQSVEKVELNSGRGIIYDRNNKKLTDTSKSQVLIVEKEKLNNNYKILELIKK
                     ATKMNDLDIYKAVQEQLTRPIIQIQTKNIDKSMKKELEKNGIMVEEKTMRYAKDGLLS
                     HTIGYIKEDDKSGQSGIEKSMDSVLRNSNEKYISAFKAGDAGNEKSLNILKGSVKTVD
                     NKDKDRHLKTTIDYNIQKKLEQILNKEENPTAAIISEASTGEILAMCSRPNFDQNDIS
                     KSLKGKNGEFENRVIKATYPPGSVFKMVVLFSALENGVIDENYTYNCTGKTKVGNTNE
                     ILRCNKRDGHGFQNLRQAFSNSCNPAFLDIAMKLGKEKILKSAEKLHLFEKVDIGLDE
                     EKIREAPKNISIRNLAIGQENIEFTPLQINQMTQIIANNGTFKPLYLYKSLVDNNMNT
                     IKTYKSSKKEELISPYVCTQVKEYMKSVSRIGTAKDLKDIEGGCGVKTGTAQSSLNKK
                     AIDHGWITGFYPEERPKYVITVLVEGTQKGNKSATPIFKEICESIK"
     gene            complement(106398..107039)
                     /gene="sigK"
                     /locus_tag="KR250_000222"
     CDS             complement(106398..107039)
                     /gene="sigK"
                     /locus_tag="KR250_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018589741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigK"
                     /protein_id="HBH0066194.1"
                     /translation="MAALKSFEKPLTPEEEIEYLTKFKIENDKSAKDTLIERNMRLVA
                     YIAKKYNNSTEDQDDLISIGTIGLIKAIETYNIDKGTRLATYASRCIENEILMNIRSN
                     KKNKTQVSLQDPIGTDKEGNEISLLDILGTEANYVLDEVELKVQVGKLYEQLNKILTP
                     REREIVQLRYGLTPYGYKTQREIAQKLDISRSYVSRIEKKALKKLEKELVQES"
     gene            107606..107968
                     /locus_tag="KR250_000223"
     CDS             107606..107968
                     /locus_tag="KR250_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0066195.1"
                     /translation="MKIRKSGFISIECIISIAILYVAVYLVSTSLYNCYSFISRNISD
                     REMLSTAKKYIEDEKYRIQNSKYELIEDKIEKNYINGYEINSRIEQILDYYQCYEINI
                     EIKNEFKKLRFNSYVTRK"
     gene            107952..108659
                     /locus_tag="KR250_000224"
     CDS             107952..108659
                     /locus_tag="KR250_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0066196.1"
                     /translation="MLLENKGSILIITLIIFSIISTVCIMCIGLIYTHNNMFELGYKD
                     IQLKEISLSGIEISVSNVLSCVEEAIDISNNEDEFREYFLGNNMIECIKKIKDTSYSS
                     LEELSLKVENNTIYDRGDFFEFDIESNVREKEFLKAIKVRVKIKNPWIGIDIEDYMDK
                     SNNNKIDDKTFNNYKASENNEIINQFKNESENIRNQEVVEFTEEDEEELKQNIIENIK
                     KNFDEKNLVQVYKYRGV"
     gene            108679..109098
                     /locus_tag="KR250_000225"
     CDS             108679..109098
                     /locus_tag="KR250_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0066197.1"
                     /translation="MTVIELIVSISIILLIVTLSFPKDNLENHKINLFARQLCSDIRY
                     VRRINMFSNYNTYIYYINQGKHEGYVLRKDGKNIKSIMLPKNAKIARSIEIIKFKSDG
                     SPQKASTIEVYNNRLRKTITITPVSGRVLLKEGKYET"
     gene            109088..109612
                     /locus_tag="KR250_000226"
     CDS             109088..109612
                     /locus_tag="KR250_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0066198.1"
                     /translation="MKHKYGYLLLESVVSLSSMVIIILVLYSIFLSTINLKLKVEDKI
                     ELQQQSLEIIKSMEGIISNSMGIMNVSNYEETFKKTTSIKCRYLDENNNEEGISNKEI
                     ILNERRNKLFVNSLNGESSQAGGYEIGDYVDEMYVSITNNGQYVNIKLKLSKCSQKYE
                     TDFKIKVWNFSESI"
     gene            109924..110481
                     /gene="efp"
                     /locus_tag="KR250_000227"
     CDS             109924..110481
                     /gene="efp"
                     /locus_tag="KR250_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HBH0066199.1"
                     /translation="MVSAGDFRKGVTFEKDGQPCLVVDFQHVKPGKGAAFVRTKYKNL
                     KTGAIREESFNPSEKFPKAVIDTRQMQYLYNDGELYYFMDQENFEQIPLNYEQVEDAI
                     KFLKENEVATIRFYQGQPFQVEAPNFAELEVTDTEPGIKGDTASNVTKAATVETGAVV
                     QVPLFINTGDKVKIDTRTGEYLSRV"
     gene            110573..111028
                     /locus_tag="KR250_000228"
     CDS             110573..111028
                     /locus_tag="KR250_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBH0066200.1"
                     /translation="MKHLYEEVAYLKGLAEGLEISAESKEGKMIHKIVDALEVFADAI
                     VTLDEEQEELQDFVESIDEDLADLEEDIADMEEDLYEEDDDEDDEDFSYIEMECPNCG
                     ELVEIDEDLLYDDEVDVVCPDCKAVILSSEDDCDDDCTCGGCSSCDDRE"
     gene            111191..111901
                     /locus_tag="KR250_000229"
     CDS             111191..111901
                     /locus_tag="KR250_000229"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HBH0066201.1"
                     /translation="MKISKKLLDNIQRFENVINYKFKNKEYILEALTHSSYSNENKKY
                     NFNERLEFLGDSVLGIVISDYLFNEEANLPEGELTKLRANIVCEDSLSEVANDINLGI
                     HMLLGRGEEATGGRHRTSILADAFEAVIAAIYLDGGFESARQFILHHMENIIYDSRKG
                     NIFRDYKTHLQEVLQGNGENNIWYRLIEEKGPDHNKRFVMEVGINDDVLGIGEGKSKK
                     EAEQLAAKIALKKKLWEK"
     gene            111955..113025
                     /locus_tag="KR250_000230"
     CDS             111955..113025
                     /locus_tag="KR250_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBH0066202.1"
                     /translation="MKKRIIPIFVPHKGCPHDCIFCNQKKITGVSTDVTSEDARNIIE
                     ECLETIDKDADVEIAFFGGSFTAIDIDIQKSLLSVAKEYVEKGLVKDIRMSTRPDCID
                     EDILSMLKEYKTSIIELGVQSLDEKVLLDSIRGHQSEIVYKSSKMIKNSGIKLGLQMM
                     IGLPADTEEKCIFTAKKFVELKPDCVRVYPTLVVKDTGLEKLMEQNKYNPFTLEESVN
                     IVKKVLVLFYVNNINVIRVGLQATDDIQIGKAVLAGPYHPAFRELVEADMIKDYLEFV
                     ILQNKNIKQMLVKSNKKNISKIIGNKKTNVKYMEEKFGVLLKTQESDLDINQLEIVLD
                     GKSLISANMRDIHRKLYDIYDL"
     gene            113053..116607
                     /gene="smc"
                     /locus_tag="KR250_000231"
     CDS             113053..116607
                     /gene="smc"
                     /locus_tag="KR250_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HBH0066203.1"
                     /translation="MYLKRLELKGFKSFPVKTDIIFKEGITAIVGPNGSGKSNISDAV
                     RWVLGEQSIKSLRGDKLEDVIFAGTDTKKPMNYCEVALTIDNSENQLELDFTEVTIRR
                     RAYRNGESEFFLNNKSCRLKDIKEVFLDTGIGKDGYSIIEQGKVDEILSNNPLSRRKV
                     FDEACGISKYRYKKQEAERNLSNTKENLERIDDVYIEIENQLKPLFNQQTKAKKYLEI
                     SEKLKTLEVNSFIREIEGIEKELSEVNEHRKVIEKELNEKEEQKNVVEKKQEDINKEV
                     EVLQDVIEKSVDYINSIKGVISKKESQINLIKERIRNFTNEISRKNLEINDIKEKLNE
                     NKQYIKELESNKLSGSEELSTLQENIKVLEASKDKQKIKLESLNNEIELLKESIIDIL
                     NKKQEFSNKLSTLNANKENMNIRDENINSEITELNKNIEIKSSELDTINKEFNMQNEN
                     LKNVNNRHKELSTNLQDSISEHNKLEDEIQKSKYNLNGYNSKLNVYIDMENHYEGFNR
                     GVKEVLKNKNLKGVHGALGQIINVPEKYEKSIEAALGAYMQNIITDNEFSAKSAINYL
                     KQNNLGRVTFLPLNIIKSNKISLGNLKANTKFIGIASDLITFDEKYRNIIENILGRTI
                     LINNIDEGIKFAKETGHRFKIVTLDGEILNPGGSLTGGSLKTNGNILSRKRYINEYTE
                     KISNIKNEISHLELKRESLDKDVKNIKNEIDSHESKIKDLEKSIIIKSTSIKNVESEI
                     ESLKGSITKLENEKNDLNSNLNYTLEKSDDVRKDMEELDDLYNKNKEKIDALNEEIKR
                     YNDLYDKEKSEFDELNLSLVKKTEVYNSIVRDIKRISGENCELEEKNKQLEESLNYEE
                     HEIIKLQDSILTEEKEKENLTKQLGDSNRNLETRKIAKDDLKNSFDEINKELKTIDRQ
                     HIELKESLFKVGGRLERLKTSQDTYINKLFEQYDMTLVQALEIKDEDLDIDRKFLESL
                     KREIRSLGNINIDSIKEYEEIKERYDFYSEQKQDLEESMEEIEKLIHTLEENMKSEFE
                     IKFEEISKNFKYVYKRLFGGGCGELTILDKENLLESDILITAQPPGKKMKNLNLLSGG
                     EKALTAISILFAILITKPTPFCILDEIEAPLDDANIFRFGEFLKDLSKETQFISVTHR
                     RGTMEAADYIYGVTMQEKAISKVISLKLKEAQEITDII"
     gene            116634..117914
                     /gene="ftsY"
                     /locus_tag="KR250_000232"
     CDS             116634..117914
                     /gene="ftsY"
                     /locus_tag="KR250_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HBH0066204.1"
                     /translation="MLKKLFGFGKDKEKEIEKKDAEEEIEVEDSVDNLENLEETIFSG
                     LEEEVIDKVEDVEEKNEESVNNEGTEEIENFEKIEVEMDSNGKEVENISNNDSQEEII
                     EELENYDEIESKEIEDKEDKKVNLFERLKQGLTKAKQGITDRIDEVLKSYTKIDEELL
                     EDLEEILITADVGVNTTMDIIERLRDKIKQKGITEPIKVREELKSIVEDILTNENSTL
                     DIEPVPCIILMVGVNGVGKTTTIGKLANRYKKDGKKVLLAAADTFRAAATEQLEIWAN
                     RTNVDIIKHQEGADPGAVVFDAIKAAKARKTDVLICDTAGRLHNKANLMNELGKVFKI
                     VDREFPEAKREVLLVVDATTGQNAVVQAKTFKEVADITGIVLTKLDGTAKGGVVLAVK
                     SEVDVPVKLIGVGESVEDLQDFNAKSFSDALFGN"
     gene            118017..118385
                     /locus_tag="KR250_000233"
     CDS             118017..118385
                     /locus_tag="KR250_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HBH0066205.1"
                     /translation="MNIEKMVEIGLLFEQYKELLTDKQKEIVALYYEEDYSLGEISEN
                     LNVSRQGVYDTLKRSEKILRDYEEKLHLVSKIQEQEKNIKIIKDKIIDIKEDLLHNRD
                     CANLIPKLENIEDVCREMIK"
     gene            118382..119734
                     /gene="ffh"
                     /locus_tag="KR250_000234"
     CDS             118382..119734
                     /gene="ffh"
                     /locus_tag="KR250_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HBH0066206.1"
                     /translation="MIFEGLSDKLQGAFSKLKSKGKLTEVDVKNAMREVKLALLEADV
                     NFKVVKDFIKKVQERCVGQEVMQSLTPAQHVIKIVNEELTSLMGDVQSKVMISSKPPT
                     ILMMVGLQGAGKTTTAGKLGGYFKKQGKKPLLVACDIYRPAAIKQLQVVGEKLDIPVF
                     NMGDKENPVNIAKAGLSHAIKNANDLVIIDTAGRLHIDEVLMDELKSIKSEVKPHEIL
                     LVVDSMTGQDAVNVAESFNEALGVDGVVLTKLDGDTRGGAALSIRAVTQKPIKFMGMG
                     EKLDDIEPFHPDRMASRILGMGDVLSLIEKAQENIDLEKAKELEQKIKKQELDFEDFL
                     QQMEQIQNMGPLDKILGMIPGMGNVKDQLGDVDLNGKEMKRTKAIIQSMTTYERRNPG
                     VLNASRKKRIAKGSGTSVQDVNRLIKQLNEMKKMMKMFAGSQKSMKKRGGLGGLPFFK
                     "
     gene            119772..120044
                     /gene="rpsP"
                     /locus_tag="KR250_000235"
     CDS             119772..120044
                     /gene="rpsP"
                     /locus_tag="KR250_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HBH0066207.1"
                     /translation="MAVKIRLKRMGANKKPFYRIVVADSRAPRDGKFIEEIGYYNPIS
                     EPKQVRINDEKAIKWLATGAQPTEVVKKLLVKNGVIEKFEASKQAK"
     gene            120109..120336
                     /locus_tag="KR250_000236"
     CDS             120109..120336
                     /locus_tag="KR250_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HBH0066208.1"
                     /translation="MKELVVDIAKALVDNPDSVVVEEFEDNDGIVLKLTVAQEDMGKV
                     IGKQGRIAKAIRTVVRSVANRENIKVSLEIV"
     gene            120444..120959
                     /gene="rimM"
                     /locus_tag="KR250_000237"
     CDS             120444..120959
                     /gene="rimM"
                     /locus_tag="KR250_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA processing protein RimM"
                     /protein_id="HBH0066209.1"
                     /translation="MKEKLTHFKVGQIVNTQGLKGEVRVYPLTDDIERFDELKDFYLG
                     KDLNNKLAVEKVRYKGSMVIMKIKGIDSIEKAEKLKNKFMYVSREDSRELEEGEFFIA
                     DMIGMEVLTVDGKYVGILEDVLQYSANDVYVVKGEEDKEFMIPAIKKFVPTIDIDERK
                     MIIDPIKGMID"
     gene            120968..121663
                     /gene="trmD"
                     /locus_tag="KR250_000238"
     CDS             120968..121663
                     /gene="trmD"
                     /locus_tag="KR250_000238"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HBH0066210.1"
                     /translation="MRFHIMTLFPEIFNSYMDESIMKRAVEKGIIEVHIYNIRDFSNN
                     KHKKVDDYPFGGGAGMVMTPQPIYDTYKHIITTHNINNPSVIYLTPKGKVYNQSMAKQ
                     MSLKEDIILLCGHYEGIDERIIDLIVTDEISIGDYVLTGGELPALIMIDSISRLIPGV
                     LNQEESFEEESFKDNLLEYPHYTRPRDFEGLKVPEVLLSGNHKKIDEWRREESIRITK
                     ERRFDLYKKSNEK"
     gene            121848..122198
                     /gene="rplS"
                     /locus_tag="KR250_000239"
     CDS             121848..122198
                     /gene="rplS"
                     /locus_tag="KR250_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HBH0066211.1"
                     /translation="MNEMLRAIEQEQLKNEVPNFGPGDTVKVHVRIIEGKRERIQVFE
                     GVVLKRQGGGARETFTVRKMSFNVGVERTFPVHSPKIEKIEVTRKGKVRRAKLNYLRG
                     RVGKAAKIKEARNK"
     gene            122519..123415
                     /gene="ylqF"
                     /locus_tag="KR250_000240"
     CDS             122519..123415
                     /gene="ylqF"
                     /locus_tag="KR250_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HBH0066212.1"
                     /translation="MSNEYEEYLMDDNLHINWYPGHMKKTKELVKNNLKLIDVVIELL
                     DARIPFSSKNPDIDRLVGDKPRVVVLNKSDMADRDKLNQWIEYYKKINIKAIPVDTIK
                     GVGINKIIEECKNVTREKMSSLKDKGRKERAIRIMIVGVPNVGKSSLINKLTGRKSTQ
                     TGDKPGVTKGKQWVRLKGNLELLDTPGILWPKFEDQEVALNLAFSRAIKDEILDTETL
                     ALRLIEKLMKIEPEKLKARYKLDCLGETPIETMDMIGHKRGFITGKKELDYTRIATTV
                     LNEFRDGKIGNITLEVPENVKR"
     gene            123804..125096
                     /gene="brnQ"
                     /locus_tag="KR250_000241"
     CDS             123804..125096
                     /gene="brnQ"
                     /locus_tag="KR250_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HBH0066213.1"
                     /translation="MGKTKDVIVFGFALFAMFFGAGNLIFPPYLGIITGPEWLIAFLG
                     FTFADAGLALLAVMATAKFDGNVVEMFKRCGMKLGILIGCADILCIGPFLAIPRTGAT
                     TYEMGIMPLFGTSIPVLLFCILFFALSYVLTIRPSKVVDIVGQFLTPALLIALAFIII
                     KGIISPLGDIVDKPMIPNVFAEGIGQGYQTMDAFAAIALASVLIVSLNDKGYSTISDK
                     LKMIGKAGVLACGGLALVYGGLCFLGATVSTMYGTDAVQSQVIVNITEGLLGNVGKAI
                     LAVVVSLACLTTSIGLTSATGQYFSRLTKGKLSYEKIVLAVSVFSAVVASFGVGTIIK
                     IASPILSIVYPPSIVLIILAFFNEKIKNDNVYKGAVYMSLLVSILTVISSYGVAVPVV
                     NSLPLNSLGFNWVVPVIIAGIIGNFIPSKSQSNTLGTN"
     gene            126214..127506
                     /gene="brnQ"
                     /locus_tag="KR250_000242"
     CDS             126214..127506
                     /gene="brnQ"
                     /locus_tag="KR250_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HBH0066214.1"
                     /translation="MGEKQINFKDVIVIGFALFAMFFGAGNLIFPPYLGVLSGSSWLI
                     AFIGFLFADGGLALLAVIAATKFNGDTSKMFSRAGKGLSIALGCAMVICIGPLLAIPR
                     TAATTYEMGILPTIGSGISPVIFSIVFFALVLVLTIRPSKVVDIVGSILTPALLIALA
                     VLIVKGIISPLGEIRDSSLIQNVFAEGITQGYQTMDALAASVFASIIIMSVIAKGYTG
                     EKEKMKATISAGVIAVIGMALVYGGLCYLGATVSQQYGQDVQQTALIVAITAALLGTT
                     GKILLAIIVALACLTTAIGLSSAAGQYFSTLTDGKLKYEHIVIVVCVFSAIISNFGVS
                     TIIQFSSPILSMVYPATITLVILALFSNKIKNDNVFKCAAYMALLVSVLTVATSFGVK
                     IPLVNSLPFASLGFNWIVPVVIAGIIGNFIPSKSSKTA"
BASE COUNT        51400 a        13210 c        23650 g        39358 t
ORIGIN      
        1 agaataactt ggctgtttca aaaatgtgaa atcattttag aaacagctta tttttttata
       61 aaaataaaga aggcaacggg aagccttctt tcgtgtaaaa aaagtttaat tttttgttaa
      121 gatttgtttg ttattttata ttacctttat aatatgtttt tatttggtgg ttggcaatgg
      181 attttgcaca actggtcatt attaatgtaa aagtggaata ttattaataa ataataatta
      241 caaaattgta atctttattt attaaataaa tgagtaaaat aatatattaa tgggtatata
      301 attaagacaa tactagtttg gggaggataa aattatggat tataaaacaa tcatattaaa
      361 tagaaagtct gttagagaat ataagaacac agatattaaa gaagaatatc ttagtgaaat
      421 taagaaatac tcagaatctt gtaagaaact agttccagaa atagatgttg atgtaagaat
      481 tatgaataga agtgaagttt ttaataattt agataaagta gcagggtaca aaggaaatat
      541 gatagattct ccaagttata ttataatatt atctgacata aaagataatt acattgaaaa
      601 ttcagggtac ataggagaaa gtataatctt taaagtaaca gaacttggaa ttggttcttg
      661 ttgggttact tttgaagatg gaaaaaaggt aaaagaaaaa cttggaatta attcagataa
      721 agaggtaaca ggaattatag ctattggata tggagaaaat gtaagtaatg ctaaagtatt
      781 aaatgctaca aaaactgggc aaaattattc aaaatctgat atgcaagttg taacagataa
      841 ggggtctact agactaggtg tagaagatgt tgtttatatg aacgaatggg gaaatgttgc
      901 aagtatagaa atcttagaag aaagagcact tcttgatgca tttcactatg caagattagc
      961 tccatctaca ttaaatagac aaccatggag atttatagtt gatggaggca ctgtagtttt
     1021 agctgttaga aaagatggtc atactaattt atatgaagaa aaaatagaca ttggtatagt
     1081 tatgctttat tttgcaacaa taataagtgc aactatgttt gatttaaaat ggaatttagg
     1141 tactccagac aaagattata aagtgccaga agactataag atagtaggat attgtaatat
     1201 ataagatagt gtaatactta atttaactac acttaaatta atttttataa aaagttcaat
     1261 aacattttac aatataatgt tattgaactt ttttattgta taatacaatt tttatattta
     1321 ttgtattgat tgaaaaatgc ttataaattt aaatgcttac aattttgtgc atgaatagag
     1381 aggtatatta ttttattaaa ctgtataatt tgcttggagg taatatcaaa tggaatggat
     1441 aaataaatta aatcaagcta ttacttatat tgaagaaaat ctcgaaagtg gagtggatta
     1501 tgcagaagca gcaaaaattg catgttgttc aacctttcat tttcaaagaa tgttttcata
     1561 tattgcagaa gtaccattat ctgaatatat tagacgaaga cgtatgacta aggctgcatt
     1621 tgaacttcag aacagtaaca tcaaaatttt agaactatct gagaagtatg gttacgattc
     1681 accaacatct tttaatagag cttttcaaaa tatacataac atttctccat ctgcagctag
     1741 agcaaagggt gttgttttaa aagcatatcc taagatgaca ttaagcattt atgtaaaagg
     1801 aaatatagaa atgcaatatc gtattgtaga aaagaaaggg tttcgtattg ttggtataaa
     1861 agaaagtatg aacatgattg tagaagagtg ctttgagaaa gttcctaagt tgtgggctaa
     1921 atgcataaaa aatggaacaa ttgataaact ttcagagtta ataaataatg aaccttgtgg
     1981 attgcttgga gttagtgtgt gtacaaatag taagggttta gattattata ttgctgctcc
     2041 aacagataag cctgtattag aagatactca tgaatactta ataccatcag gcacatgggc
     2101 agtatttgaa tgtgtttctc caatgccaaa tgctctggca atacaggaat tgcaaaaaag
     2161 aattattact gagtggttac caagttctgg gtatgtgtat gctaatttac cagatattga
     2221 actttatccc aatggcgata tcaatgctcc tgattatact acagaagttt ggattccaat
     2281 tcagaaacca agttaaaatt aataaataat atggaattaa taaataacat taaaattaat
     2341 aaacaacatg aaattctaaa attacaggct gaaaatagtc tgttattttt ttgtaaatat
     2401 ggaaagtaag ctttacataa atggaatata gttgtataat tatggaaagt agactttccg
     2461 tagttgagat attaaagggg gatagatgtg aaaaatagat tagaagaaat aagaaaagaa
     2521 agaggcataa aacaagaaga acttgcttct attttgcaag tatcaagaca aactataggt
     2581 tcattagaaa atggtaggta taatccttct atcatcttag cctttaagat agctagatac
     2641 tttgatatga gtattgaaga aatatttatt tatgaggagg agtaaaaatg aaaaagaata
     2701 tgttatatgt tggaattata tattttattt gtggaatttt acttgtttta ttggctacat
     2761 ttactgagtt ttcatttgag gcttttattt ggggattatc tggagctgct ttaggtccag
     2821 gtgtatgtat gattcttcaa tatatgtatt ggagtaaacc tgaaagagca gtagattatg
     2881 aagaaaagat aaaaaatcaa aggatagaga tgaatgatga aagaagaatt atgttacgtg
     2941 ataaagctgg acgtattact aatcaaatca tgtgctatgt tcttgtaatt cttatattta
     3001 ttgtttctat attgagtgta ttttctgtaa tggtaataag caagtgggtt ttagttgttc
     3061 ttggattgct tattttattt caatttattt gtagtgttgt tgtatacaat aaattagata
     3121 aaaggttgta ataagttatg atataaaata gaaaattata taaaagtaaa ttagttttta
     3181 aattaatgct agaagataaa tttatataat tatataaatt ttaaaaagac acagtaattt
     3241 caaaaaataa cttaaaattt aaatcaaaaa taaaaaaact cctaatctat tgaataaata
     3301 gctttaggag tttttaatgg tgcccgaggc cggactcgaa ccggcacgga gtattagtcc
     3361 ggtggatttt gagtccactg cgtctaccaa ttccaccact cgggcatgtc atgtatatta
     3421 ttatagcaaa aaaatatatt gtttgcaaca tatttaattt taatatacta ttattattat
     3481 gggaatatta aataacacta tattggtata taatatatat gttacaaaaa tagataattt
     3541 ttaggagtga tataatttgg aaaaaaaatt aattataata gatggaaatt caataataaa
     3601 tagagctttt tatgctcttc cagaaatgaa taataaagaa gggttaaaga ccaatgctat
     3661 atatgggttt actacaatgc tatttaaaat gatagatata tataaaccaa cacatataag
     3721 tgtagctttt gacagaaaag cacctacatt taggcattta gaatataaag attataaggc
     3781 tggaagaaag ggtatgccag atgaattggc agaacaatta cagccattaa aagacctttt
     3841 agataaattt agaataaata gactagaaat agatggttat gaggcagatg atattatagg
     3901 tactgtttct aaaaaagcag aagaaaatgg atataaggta tacatagtaa ctggagataa
     3961 agatgctatt caacttgctt cagataatac aactaccctt ataacgaaaa aaggggttgg
     4021 agaagtagaa gaatacaact ttaattctgt aattgaaaaa tatgagatga ctccaacaca
     4081 atttatcgat ttaaagggtc ttatgggaga caagtctgat aatatacctg gagtacctgg
     4141 aataggtgaa aaaacaggta taaagcttat aaaagagttt tctagtatag agaatctaat
     4201 agagaataca gataagctta aaggtagtgt aaaaaagaaa atagaggaaa acaaagaaat
     4261 agctattcaa agtaaaagac ttgcaacaat tataagagat gtgccaatag aagtggattt
     4321 agatagtatg atttttggcg attatgataa agatgaactt ttagataaat ttagatactt
     4381 tggatttaca agtctcttat caagattagt tgatttggtc gattcagatg acacagaaca
     4441 cgtagaagaa aaaatagaaa tattaaaatt aaaagatata gaaaaattta tagatgaagt
     4501 aaacaaaaaa ggacaagtta ttttaaaaac tgttagagga caaggtaata tacttgaaaa
     4561 aaatataata tatattttta taagtgtaga tggagaaaaa atatattatg tagattcaaa
     4621 tgaagtgtca aagttagata gtatattttc aaataatgaa attaaaaagt atggatataa
     4681 tttaaaagat gattatatat ctttaaaacc atataatata aacttagaaa atatgtattt
     4741 tgatataaca atagctgagt acttaataga ttcaacttca tctacctcat atgattgtag
     4801 tgcaatagct atgaagtatt tatcaaaaaa ggttaaatca aaagaagaat tattaggaaa
     4861 gggtgttaaa tctaaaaact atgaggattt agaatttgag gatttagcag agtatatggg
     4921 tcaaataata tgcacagtta aagaaacagt tcctatgatg gaaagaagtc taatggatat
     4981 gagtatggat ggtctttttt atcatgtaga gatgccttta gtagaagtct taggtcatat
     5041 ggagtatgaa ggtataaaag tagacaaaag tatattggat gaattgagtg ttgaatttaa
     5101 agaaataata gctacattag aaaaggaaat ttatgaatta tcaggagagc cattcaatat
     5161 aaattcacca aaacaattgg gtgttatatt atttgaaaaa ttagagcttc caataattaa
     5221 aaaaactaaa actggatatt caactaatgc agatgtttta gaaaaattaa gagataagca
     5281 tccaataata gataagataa ctgagtatag acaaatagta aagttaaatt ctacttacgt
     5341 tgaaggactt ttaaatataa taaatccaga aagtaataga atacattcat catttaatca
     5401 aactataacg actacaggaa gaatttcatc tacagaacca aatcttcaaa atatacctat
     5461 aaaaatggaa atgggtagaa aaataagaaa agtgtttatt gcagatgata actgtaagtt
     5521 ggttgatgcc gattactcac aagtagagct aagagttctt gcacatatga gtcaggatga
     5581 aaatatgata gaagcattta aacatcatga agatattcat accaaaacag cttcacaagt
     5641 ttttaatgtt cctatggaag atgttactag tgaattgaga ggtgcagcta aagctgtaaa
     5701 ctttggtatt atatatggta aaagtgactt tggtctagct gatgatttaa atatacctgt
     5761 tccaaaggcg aaagaatata tcgaaagtta ttttgctaaa taccataaaa taaaagagtt
     5821 tatggacaca acagtagaaa aggctagtga ggatggatat gctgtaacaa ttttaaacag
     5881 aagaagatat ataccagaaa taaagtcaag caattttatg gaaagaaata gaggaaagag
     5941 atttgcaatg aatgcaccaa tacaaggaag tgcagcagat ataataaaaa ttgctatgat
     6001 aaatgttcat aaaaaattgg aagagaacaa tttgaaatca aaacttatac tacaagttca
     6061 cgacgagttg atagttgaag caattgatga tgaaatagat atagttaaga aaatagtaaa
     6121 agaagaaatg gaaaatgctg taaatatgga tgttcactta gatgtagatt taaatgtagg
     6181 tagttcttgg tatgagacaa agtaggtgat tttatgttga tattaggact tacaggagga
     6241 ataggctgtg gaaaaagcag cctatcaaat atatttagaa atcttaacat tcctatagtg
     6301 gatgctgata ttatatccag gaagatattt gaggataagt tgttattgga aaaagtattt
     6361 gtccattttg ggcaaagtat aaaaaatgat gatggaactc taaatagaaa agctcttggg
     6421 aaaatagttt ttagtgatga agaaaaactt aaggaattaa ataacttgac tcatccacga
     6481 ataagagaaa aaataataag tgaaatagaa aaattgagaa aaaaaggcga aaatatagta
     6541 gtgttagatg cggcaatatt ggtagaaagt ggttttttag atatggttga caagctatta
     6601 gtagttactt gtaaacaaga agtacaaatt agtagaatac aaaagagaga taattgcagt
     6661 gagcaggaag cacttagtag gataaattca cagatgagtc aggaagaaaa atccaaatat
     6721 ggtgattata taatagataa ttcgggaaca ataactgaat tagaaagtaa agcacataaa
     6781 tttattgagt acatgaagga gaattggcgt gaatagtaag aaagtattga ttctatctat
     6841 ttttataatc ttatttgggg cactattaat ggaaagcaaa gtaatacata aatttttata
     6901 tcctaaaaaa tattcagagt atgtagaaaa gtattcgaaa gaatttaatt tagatgaaaa
     6961 tatagtttac agtgttatta aagccgaaag taagtttaat agttctgctg tttcaaaaaa
     7021 ggaagcaaaa ggattaatgc aaatattaga cataactaga gattggggag cagaggaact
     7081 aaatttaaaa aatgtggata ttttcgaccc agagactaat ataagacttg gctgttggta
     7141 tttaagtaag ttatacaaag aatttggtaa attagattta gtgatagctg catataatgg
     7201 tggttcaggt aatgtgaaaa aatggttaga aaataatgaa tatagtaaag atggcgaaaa
     7261 tctacatgat atacctttta agcaaacttc aaaatatgta gaaaaagtaa aaaataatta
     7321 cgaacattat aataagatat atggcaagaa aggaaaaaac taatgaagag aataaaagtc
     7381 ttaacagtcg ttttagctat aactcttatg gtagctggtt gtagtaatac taaaacaaaa
     7441 caaagtagta gtgattcaag tttacaaagt caaagttcta tagatgctaa gtatataaac
     7501 ttaacaatgg taactccaaa aactataaat cctataacta atactgataa gtctgttggt
     7561 tatataatga atttagttta tgatagttta tttactatag atgagaacta caatgtaatt
     7621 cctcaattag ttaaggagta taatatagct caggatggaa tgagtataga tataaagcta
     7681 aaagatgcaa agtggcatga tggaaaaaat attacttcaa atgatgtaaa ctatactatt
     7741 ggtttaattc aaaaaagtgc agatagtcca tataatgaat ttactaaaaa tatagcttct
     7801 gtgaatatta aatcagataa agattttaca ataaaattta aagctagata tgctttttca
     7861 attgatagtt taatttttcc aattgtatct caaaatcatt tagattcaaa agatgtaaat
     7921 gacaagaata ataatatgat aggaaatggg aaatataaaa tagaatcata tacagaaaga
     7981 gaaggaatgg ttttatctgt aaacaaagat tactatgaag aagttccaaa aaccatgaag
     8041 aacataaaag taggtatggt gcctaatgaa gatgcaagga catctatggt tatggctctt
     8101 gatagtgata taaccaatgt tacattaaat gatttaagta agtttcaaga aaaagagttt
     8161 aatataacaa aatatcaagg aagagattat gagtgtgtat tatttaatta taataatcct
     8221 tttttcaaag atgttaattt tagaaaagca attgcacact ctatagataa agatagaatt
     8281 attagtgaag gttatatgga tgatgctact ccagttaatt ttccacttaa ttcaaaatca
     8341 aaatactata atagtgaaat gaaggattta gagtttaata aagataaggc gatagagtgt
     8401 cttgctaagg tagaatatgc aaatgtaaac tcagtaaatc aaaatgacaa taaagtaaaa
     8461 aatcagaaaa aatcagctaa aaagaactca aagaagctta cgccagaaga agaagcaaaa
     8521 gctaaaaaag cagaagaaga tagaatcaaa aaagaagctg aagagaaaaa aaatagagaa
     8581 aaagaggaag taaagaaaag tctttctgaa atgaacttaa agataatagt aaacagagat
     8641 aatagtgaga gaataaaaac tgcaaacata ataaatgaaa acttaaaagc tataggaata
     8701 aatagtacag taaatcagct gtcagataag gatatggaaa atgctcttaa ttctaaaaat
     8761 tatgatttag cattagttgg atggaaacta tcaattatac cagatgcaag tagtattata
     8821 gcgagtagtg gatatactga tgataagtta aatggatata tgtctgcact tacaagttct
     8881 acgtctgaaa ttgaaacaaa aaaagcatac aaagatgtac agacatatat aaaagataat
     8941 gcaacattta taagtttagc tgtgagaaac aactatattg taagtaatag tagattaaaa
     9001 ggtaaaatta ctccaaatga ttttgatgta tatgagggaa tatctaattt agatataaaa
     9061 tcgaatgaat caaattaaat gattaatttc tatttaaaaa gtataaacta tatgatagaa
     9121 tacccttata gggtataagg aggtaattaa atgaagaaga aactattagt agaaggtatg
     9181 agttgtggtc attgtgtaaa ccatcttaaa actgcattaa cagaagatat agatggtatt
     9241 gaagttttag atgtagattt agaaaataag tgtgcatctg tagatatgaa agatgatgta
     9301 tctattgaaa aattaaaaga agtgatagca gaattaggat ttgaactaaa gggaattgaa
     9361 tagaaattga taaactaaaa aaagacctct attgggggag taatagaggt ctaaaaatgg
     9421 gggagattga gtatgtttga ttttacatta ctattatctc cccttttaaa ttaatatata
     9481 cataaaaagt aaaaaaattt tgtaatgact cacatttctt gagctttttg aatatactat
     9541 aaatagctaa gggagggagt atcatggaat ctaggggata taaagaggat ttaaaaaaaa
     9601 tgatacttga tatggcccac aaagaacttg agagttatgt atcacaaaaa ggagcaaaaa
     9661 aatactttga aggtaacttg gataagatta ttaataataa tataaaaaaa ttcaatcaaa
     9721 gtattggtct taatagagta tatttaaaaa tgttaaaaaa gtgtgcatat caagaaagtg
     9781 taacttcaat atcaagaact ataaagaaag aagcattatt taaatcaaaa aaagagctat
     9841 ttgctttttc agagtactta aaacttgata ttaacaagcg actttcatat aatcaaattt
     9901 taagaaaaat atcaagatat atatactaca atagaagttc atatgctcaa aaatacgttg
     9961 ttttcaaaag aggagatgaa gaatatcttt tagaaccaga gaaaataaaa gatgaattaa
    10021 tatcaagcta taggtcaaag actagagaag atatgaggtc aatagcaaaa cttttggata
    10081 tagaagtaga agatgattat aatgctgaag atattagaaa aaaagtaata aattatatta
    10141 ttaaagaaaa acttaaaagt tagagtttta aagaataagt tctgccaatt tgcatgatta
    10201 agtataactt gggtataaat tttttatgca tttgaaagag cttattcttt ttttgacata
    10261 ttcttttaaa taatttacca tgatttggta attggtcatc atcattcaaa tttttaactt
    10321 aatatgattt aaataaattt ataataatga tataatataa aaaaaataaa aattggagag
    10381 tgatttcaaa atgaagttta atttttgtca taacaatttc aatgttacta atttagaaaa
    10441 aagtttagat ttttataaaa aagctttagg gcttaaagag gtaaagcgca aggaagcaga
    10501 agatggaagt tttatattag tttatttagg agatggcatt actagtcata cattagaatt
    10561 aacttggctt agagattggg atagaccata taatttagga gacaatgaat ttcatctggc
    10621 tttagaagtt gaggagtttg atgaggcaaa aaaacttcat aaagatttag attgcatatg
    10681 ttttgaaaat gaaagtatgg gaatttattt tatagcggac ccagataatt attggattga
    10741 aatacttcct aaaaatcact aattttaagg atatgtttat aatttcttta caattaataa
    10801 gaaaataagt tatcattaaa ttgtagggac taaaaatgga taataatgtt atgaatttat
    10861 gactataaaa atttgttggg gggagtgata gtagtgaaaa aagctatagc agctttagga
    10921 attggggcag tagcagtatc tgttagctcc ataaatgcaa gtgctcttga aaagggaaca
    10981 gtaacagcaa gtgccttgaa tataagaagt ggaccaagtt ctgattgcga taaggtagca
    11041 aaattataca agggaaaaac tgtagagatt ttagaaaagt ctaatggatg gtataaagta
    11101 agggtatcga gttctgttgt tggatgggga agtgcaaaat acatatcaac aagtggttcg
    11161 tctgaaggta catcaagtca aaataatcca acttcaagtg ggacaactat tagtggaaat
    11221 ggtaaagtaa atgtgagttc tagactgaat gtaagaagtg gtgcaggtac taactattca
    11281 ttagttggta aagcaaataa tggagatgta gtaaagttac tagaacaaag caatggatgg
    11341 tataaaataa aactatcaaa tggagttact ggatgggcta gtagccaata tatttctaaa
    11401 acatcagaag atgttggaac gaacaattct agtaattcaa atagtactaa taattctgat
    11461 aaaaaaccat ctagcgaaga atcaattgaa ggcaagaatg gtaaggtaac atctgctgtg
    11521 agtttaaatg taagaagtgg tcctgggaca agctattcta taataggaaa attaaatggt
    11581 ggagatgttg ttgaattaaa agctaaaagt aatggttggt ataaggtaaa gttatcgagt
    11641 ggaacaattg gatgggtgag tgccagttat atttctgaga ctaatgagga tacaaaagaa
    11701 aaaccaaatt catcatctaa ccaaaattct caatctaata gtaactcaaa gccatcattt
    11761 actggaaatt cagataaatc tacagcaaaa ggttcaacaa tcgtagattt tgcatatact
    11821 cttattggaa taccatatca atggggagct tcaggacctg ataaatttga ctgttctgga
    11881 tttacacagt atgtttttaa acattctgta ggcgtttcta ttcctagagt ttctagagaa
    11941 caggctaatt ttggaagtgc aatttctatg ggaaattatg caccaggaga tttagtttat
    12001 tttgacactg atggtgatgg aacaactaat catgtaggta tatacgtagg aaatagtaaa
    12061 tttatccatt gtagtggtac tcagactaat cctaataagg ttaaagtgga taatttaact
    12121 agttcatatt ggtctaaagt attacttggt gcaagaagat ttgtttagta agtacaattt
    12181 cttattaaaa aattagaaga aataattatt tatattcata aattgtgact cttaaaaata
    12241 tactttatta ataatataat tttgggaggg acaaactaat gaatagaaga aaaacgatat
    12301 acagaggatt taataataat agacataaga ttgatttacg taaatatgtt ataacgattg
    12361 cttgcttgtg tctaattggc tattattctt atacaaaaat aaaagatagc aagatattgg
    12421 agtatgtatc ggctaaaata ccatttctaa ataattcatc agatataact tataaagata
    12481 tatctgatga attaaattca ataaaaaaag ggaaaaaatc taaatcacaa accaattcag
    12541 atgataaaca agaaactaat ccagaaaaag cggtaaataa cactaaagag ccagaagaag
    12601 tcaaattagc tactatagaa ggctgggata tgtatactat tcaagttgct gcaatagata
    12661 ataatgatga tttgaaaaaa atacagacat cattagttaa taatgacata cctttttcag
    12721 ttatggagaa ggatggagta aaaaaaatac agacctattc ctcttttgat gaaaatgatg
    12781 ttagaaagca gataagcagt gtaagaaagg tgtttccaga tgcattttta tcacacttag
    12841 atgcacctat gttgtcttta gagtatacaa gtaactacgc ttatatagag agtatatcta
    12901 aagaattaaa taaattaata acaaacttta aagaagaatc aagtttttgg tcgaatgctg
    12961 aaaataatgt agatatggaa aaatataata ctattttgac aaatagaaag gcaatatcac
    13021 aaaatatatc aaaagaagct gaaaagattg attattctga aatgcgttta tttaaagata
    13081 atttaataga gtatgtaaag aatgtgaatg aaaaaattga tacagcttca aaagcagcaa
    13141 atgaagaaaa atatagtgta agtaaaagtc tgcttttatc atccatgcag gagtactcta
    13201 tgtttataaa ttctataaaa taaaatacag aaaatttgga ctttagaaag ctatatgaaa
    13261 aggattgttt aattaagcaa tccttttcct caatttgact taatatagag gtgttgattt
    13321 ataaaataaa ttataaaaat acggatattt ttttaacaat tgttaagatt ttttaatata
    13381 tatagtatac ttatagaagg cagaaaaaat gtttttttat agataagggg gtaaaacaat
    13441 gaaactctta acttttaagg gagggataca tcctccatat agaaaagagt actctaacac
    13501 aaaggcactt gaaaaagctc aagctccaaa gatagtttac attcctcttc agcaacatat
    13561 aggggcacca gctaaaccta ttgttgaagt tggtgatgaa gtaaagtttg ggcaaaaaat
    13621 aggtgaacaa caaggttttg tttcatgtaa cgttcattct tcagtatcag gtaaagttat
    13681 tgctattgaa caacatgaag taccaggagg ttcagctcaa tgtgtagtta tcgaaaatga
    13741 ctttaaggaa gaattacatg agagtgttca accaaaaggt cagttagagg atttgagcaa
    13801 agaagatata gtaggtatca taaaagaggc aggaatagta ggtatgggtg gagctacatt
    13861 ccctaaccat gtaaaagtat ctcctccacc agatagcaag gcagaagtag ttatattaaa
    13921 tggtgcagaa tgtgaaccat acttaactgc agaccacaga ttaatggttg aaaatccaga
    13981 agatgttgtg tttggtttaa gagcattaat gaaggtatta gatgtcaaaa aaggatttat
    14041 aggaattgaa acgaataaac cagatgctat agaagcaatt caaaatgtag ctaaagacta
    14101 tagcgaaatc gaagttgttg gccttcaagt gaaatatcca caaggagcag agaaacaact
    14161 tatttatgcg tgtacaggca aagaagttcc atctggtgga ttaccaatag cagcaggtgc
    14221 agttgttgac aacgttgcca cagcagcaca gatagctaag tctattaaaa caggtatgcc
    14281 tttggttgaa agaatcacta caataacagg tagttgcatc aaagagccaa agaacctaat
    14341 aacaaaagtt ggaacattag tttcagaaat aatagaacaa tgtggtggat ttaaagaaga
    14401 taagaagatt ggtaaggtta taatgggtgg acctatgatg ggaatagctc aatatactac
    14461 tgaaatagcg actaacaaag gttcttcagg aattttatgt ttggatgagg aagaatcacg
    14521 tactccagat attcaaaact gtttaagatg tggaagatgt acagatgtat gtccatcatt
    14581 cttacaacca cttttcataa gtgcatattc tttaaaagat gactatgaca cagctgaata
    14641 tcatagagca atggattgta tagaatgtgg gtcttgctca tttatttgtc cagcaagaag
    14701 accattactt caatctataa gatcagcaaa gagagaaata ggagcaaaga gaagaaagca
    14761 agctgctcag aaataaagaa agttaagggg gaaaaacgat ggaaaataag ttgatagtat
    14821 catcttctcc tcatgtgaga agtaatgaag atacttcata tataatgaaa caagttatta
    14881 tagcactcct tccagcagca gtagcaggag tatacttctt tagacttaat gctttgagtg
    14941 ctatgttttt ttgtatactt ggtacagtag gtactgaatt tttatatcaa aaatttacga
    15001 agcaaaaaag tactatagga gatttttcag ctgttgtaac aggattatta ttagcattta
    15061 acgtaccagc atcacttcct tggtggatgt gtctagtagg aggaatattt gcaatattag
    15121 tagttaaaat ggtatttggt ggaattggat gtaactttgt caatccagca cttgctgcaa
    15181 gagcattttt attagcatca ttcccagtag caatgactgc ttggactcaa ccaggtgtta
    15241 actggatagg taaaaattta gatgcagtta ctacagcaac tccattaagc tttttgaaaa
    15301 atggagcagc aggactggca gatatttcta gcaatggaat tagtcttgct gacatgatga
    15361 tagggaatat tggtggatgt ataggtgaga catcagcaat attattatta ttaggtggag
    15421 tatacctaat gtataaaggc ataataaatt atgttatacc agtattttac atagcaactg
    15481 tatttatatt aacattcctt ttaggtggat ttaatataac tttcgcaata tatcaactgt
    15541 ttgctggagg acttatgtta ggtggattct tcatgcttac ggattataca acttcgccta
    15601 tgactaaaaa aggtcaaata atatacgctg tattagctgg tcttataaca actgttataa
    15661 gaatgtatgg tggatatcca gaaggtgtat cttactcaat actattagtg aactgtcttg
    15721 ctccacttat agataagttt gttagaaata gagtatttgg ggaggtggct aaataatgaa
    15781 cagtatggtg agattaggtg gtactttact tgctataagt gcaatagctg cattagctct
    15841 tggagctact aatcaggtta cagctccagt aatagaacaa agaaatatac aagcaaataa
    15901 tgaacttaga aaagcagtcc ttccagaagc aaaagaattt aaagaaatga aggaagatac
    15961 ttacaaaggc cttggagatg gtcttatagc tgaagtgtat gaaggtttag atggttctga
    16021 agtggttgga tatactttaa aagctaaacc aagtggatat ggtggagaaa ttgaagttat
    16081 ggtaggtata tcatctgaag gtcaagttac aggtgttgac attggtaata tgtcagaaac
    16141 agcaggtctt ggagcaaaag ctaaggatga tgcatttaag ggtcaataca aaggaaaaac
    16201 tgctgagcct cttgaagtag caaaaggaag tacaacagca gataatcaaa tattagcaat
    16261 atcaggtgca actattacat ctacagcagt tactacagga gtaaatgctg ctatagatgt
    16321 tttcaatagt gctcttaata aataaggagg gttaattatg aatttagcta aagtatttaa
    16381 aaatgggcta atagatgaga acccaacatt tgttcaagtt ataggtatgt gtccaacact
    16441 agccgttaca acttcagcaa taaatggaat tggtatgggt ctttcaacag cagcagtttt
    16501 aatatgtgca aatttagtta tatctttaat aagaaaaatt acaccagata agataagaat
    16561 acctatattt atagtagtta tagctacatt tgttacaata gtaggtatgc ttttaaaagc
    16621 atatgttcct gctcttgata aggctcttgg tatatatata ccattaatag ttgttaactg
    16681 tttaatatta gctcgtgcag aaagttttgc tttcaaaaca ggagctatgc cttcaatagt
    16741 tgatggtgta ggtcaaggtc ttggatttac tgttgcactt acaataatag gtgcagtaag
    16801 agaattactt ggaaatggta gtttatttgg aatgacatta tttggagcat ctttccaacc
    16861 agtattaata tttatattac caccaggtgc attcttaaca ttaggattct tatttgcagg
    16921 atttaataaa ttaagaagta aaaaagcata gtggaggtgt aataaatgaa tttaatactt
    16981 ttatttttaa gtatcgttct tgttaataac gttataacat ctcaattctt gggtatatgt
    17041 cctttcttgg gagtttctaa gaaggttgat acagcagtag ggatgggagt tgcagttacg
    17101 ttcgttttaa ctctagcttc aattataact tattttatac agattttatt agtaaaaact
    17161 ggaactggat tcttacaaac aatagctttt atattggtta tagcttctat agttcagttc
    17221 gttgaaatgg taattcaaaa gatgagtcca tctttatatc aagcattagg agtttattta
    17281 cctcttataa ctacaaactg tgcagttctt ggtatagctc tagttaatgt tcaaaaaagt
    17341 tataatttag tagaaacaat aataaacgga tttggagccg gagctggatt tactttagca
    17401 atagtaatat tcgcaggtat aagagaaaga ctagaattag ctgatatacc agaagcattt
    17461 aaaggtttcc ctataacgct tatatcagca ggtttaatgt caatagcttt cttaggattt
    17521 acaggactta taaagctata aaaaagaggg gtgaaaatgg tgatacttac agctgtatta
    17581 gttttaggaa ttatgggctt aatcttcggg atagtactag attttgcatc taaaaagttt
    17641 gctgtagaag tagacgagag agtggaagct atactaggag tacttccagg agccaactgt
    17701 ggaggttgtg gatttccagg ttgtggaggt cttgcaaatg cgatagttga aggcaatgca
    17761 ccagttaatg gttgccctgt aggtggagca gatgttggtg caaaagttgg agaaataatg
    17821 ggaataagtg ctgaagcagg tgagaaacaa gtagcaaaag ttatttgtaa aggaacttgt
    17881 tcaagtgcaa aagataaata tgagtatgaa ggaatatcag attgtagagc agcaaatgtt
    17941 ttaaactcag gtgctaagat gtgtaaattt ggatgtttag gtcttggaac atgtaaagat
    18001 gcttgtaaat ttgatgctat ttctatagtt gatggaatag cagtaataga tgaagaaaag
    18061 tgtgtaaatt gtggaaaatg taaagaagtt tgtcctaagg gtataataat cacaaaacca
    18121 gaaagtcaag aagtagttgt agagtgtaat agcaaagagt ttggtaaagc tgttaaagaa
    18181 aaatgtactg ctggatgtat tggctgtgga atgtgtgtaa aagcttgtaa atttgatgca
    18241 ataatatttg aagacaaaat agctaagata gaccctaata agtgtgtagg ttgtatgcaa
    18301 tgtgtagcaa aatgccctac aaaagtaata tcaggtgaca taactaagaa aaagaaagtt
    18361 actattgacc aagaactttg tgttggatgt acagtgtgca aaaaacaatg taagtttgat
    18421 gcaatagagg gagaattaaa agaaaaacat aaggtagatg cagataaatg tgtaggatgc
    18481 catttatgta tggaaaaatg tccaaagaaa gctattaaaa ttttgtaata tggacaaaat
    18541 aaggatatgg aataatttcc atatctctaa tgagtacata aaacagaggt atggaataat
    18601 ttccatacct ctattttatg tatatttatt ctaatggtaa taaatataac aatagtgtaa
    18661 caaaaaaggt tcgacatttc tgttgaattt tttacaatat agtgatagac taaataggta
    18721 caaattacgt acaaatgagg tgaataaatg aatataatct tggcatcagc ctctccaaga
    18781 aggaaagaaa ttctagagaa tacgaatgta agatttgata taattaaaaa tgaaattgat
    18841 gagattatat tggaaggtga aacaccaaaa catttagtta tgagattagc ttttgaaaaa
    18901 agtatgtctg tagcatctga acataatgaa gatgttgtta ttggagctga tacagttgtt
    18961 gttttagaca atgctatttt aggaaaacca aaagatgagt cttgtgcaag agatatgtta
    19021 aaaagactat ctggaagaga gcatcaagtc ataacaggta ttagcttaat aaacttgtgc
    19081 gaagataaaa aggtaattga ctatgtaatc agcaatgtga aatttaaaac gttatcagag
    19141 caggatattg aggattatct gaaaactaat gagtcatttg acaaagcagg agcctatggt
    19201 atacagggtt atggagcttt gttagttgaa gaaattagag gagattattt taatatagtt
    19261 ggtcttccaa tttcaaggtt aggcgaccta ttaaaaaaat attttagtat aaatcttttt
    19321 tatggagtgt gatttatctg aaaaagtcat ttaattcaac cattaaggta aaagaaatgg
    19381 caccagagga aagacccaga gaaaagatgc ttgccaaagg cgtaaaaagc ttatcaaatg
    19441 ctgagttgtt ggctatactt ttaagaactg gcaacaagaa taaaaatgct attgaattag
    19501 ccaactatat aataaataga gatattcagg gtattagaca tttagaagat atgacaatag
    19561 aggagctgtg taatatagat ggaattgggt tatcaaaatc aacccaaatt aaagcagctt
    19621 tagaactagg ctctagagta gctagtttta aacctataaa gtataaaata aggaatcctt
    19681 gggatataca gaggtattat atggatagcc taaggtattt aaaaaaagag gtttttaaag
    19741 cggttctttt aaacacaaaa aatgagataa tatctgatgt agatgtatct atagggactt
    19801 taagctcgtc attagtccat ccaagagagg tttttaaaga agctataaga agaagtgcaa
    19861 gtaagataat agtgatgcat aatcaccctt caggaagtgt agaaccatca agagaagata
    19921 aaaatatcac atcaagactt attaaatgtg gagaaataat tggaatagaa ataatagacc
    19981 atataattat tggagatgga ttatacttta gctttaaaga aaatatgata atttgacttt
    20041 atatgtgata aaataatagg agaatgtttt aaacatgatt cttaatagta ggaaggagta
    20101 aaatcatggc taaggaaaaa aagaaagaaa aaaaaggttt ttttagtttt aataagatga
    20161 caaaggatat gggaatagat ttagggactg caaatacatt agtttacata aaaggacaag
    20221 gtattgttgt tagagaacca tcagttgtcg caataagaga tgatagtaaa gaggtattag
    20281 cagtaggtga agaagccaaa aaaatgatag ggagaacacc aggaaatata gtagctataa
    20341 gacctatgaa agatggagta atagctgatt ttgacataac tcaatcaatg attagctatt
    20401 ttatacaaaa agcggctgat aaaaaaggtg ttgtaagtcc aagaatagct atatgtgttc
    20461 cttttggagt tacagaagta gaaaagagag ctatagaaga ggctgcaaga caagcaggag
    20521 ctaaagatgc attccttata gaagagccaa tggcagcggc tataggggct ggattaaaag
    20581 ttgaagaacc agaaggtaat atggtagttg atattggtgg aggtacttca gaaatagctg
    20641 taatatcttt aggaggaatc gttactgcta aatctataag aataggtgga gacgaatttg
    20701 acgagtctat agtggcttat gtaaaaaaag aatataatct tatgataggt gaaagaactg
    20761 ctgaaaatgt aaaaattaac ataggttcta catttaaaga tgatgaagaa ataaatatgc
    20821 aaataagagg tagagattta atctcaggat tacctaaaac aatagaaata tgctctacag
    20881 aagttagaga agcattaaaa gaacctgtaa gttcaatagt agatgcaata aaatctacac
    20941 tagaaagaac tccaccagaa ttagcctcag atataatgga aaacggaata atgctaacag
    21001 gaggaggcgc tttgcttaga ggtcttgata agcttattac acaggaaact ggtatgtctg
    21061 tacaaatagc agaaacacct cttgattgtg ttgcacttgg aacaggtaaa tctgttgaag
    21121 accaagaaat ctttgaaaaa gtattaatga tgaatactaa gaattagaag ttggtgatgg
    21181 ccttgagatt tgacaagaac aaaaatgaga aaaaaaggat taatgttaaa gtgatagcaa
    21241 cgggcgtagt tgctatcact ttaattggaa ttgtgggtat atcaattggt aaattttcta
    21301 gtggtagtcc agtaaattta ggagtagctt cagatgctat tacaagtgta ggtaagggaa
    21361 taaatgatgg attttctttt atcaaaaatg gttttaaaga tgtagcaaat tttaaagata
    21421 attctaaaaa ggtaaaaaaa ttagaagaag aaaatgaaaa acttaaaaaa aatatgatag
    21481 ctttaaatgc caaactagat aaaacagaat cgctagaaga gttaaaaaag acactgaact
    21541 ttgtacaaga agagtataag gcgacaagta tttcagcaag tgttgttggt aaaaatgatg
    21601 gaaactggta tgagagtttt gtcataggtg caggaaaaaa ttcaggtgta aaaaaagaaa
    21661 gtatagttat gaatggtagt ggattagttg gcatagttta tgaggtttcc aacaattata
    21721 gtaaggcaat ttcactattg gattctaagg catctgtaag ctttaaacta gcaaaggatg
    21781 ccaatgctaa aggtacaatt actcaaaata ctactttaga taataaagat agttataata
    21841 gtaaaggata tttgcaagga tatatgtttg actcatcata taatgtaatt caaggagata
    21901 taataacaac atcaggtctt ggattcttcc cagatggtat accaattggc gaagttgaaa
    21961 aagtggttga tgataaagat aagtcattaa aatatgtagt tgtaaaacct tatgttgatt
    22021 ttaagaatat aaatgacgtt gtagtcatag aaccaagaaa tataggataa aggggataaa
    22081 aacaatgaaa aaagttttac tttgtctgtt ggggattgtg cttatgacta tagaaaatag
    22141 tataacaaat tatatagata tttttggcat aagctttaat ttagttctta tatatgtaac
    22201 cataatctct ctttatttag atgaattaga aggtgggatt atagcagcaa taatagggct
    22261 tattaaagac ataaccgttg gtggaatttt tggtgtaaat gcattaattt tgtttattat
    22321 tgcatatgca attggataca tgagagatca gctatataaa ggtagttata ttattacttt
    22381 cgttttaatt tttataggca gtttaattga ttcaattgtc aatataggta catctagtat
    22441 aatttatcaa agctacaaca tatcaacact ttttgtaaaa ggtctactag tagcaccaat
    22501 agtgaatagt ttaattggtt tagtaatata tcgcttatct aaaagagcag ttttaaaact
    22561 taaagaagat tagttggtga tttcatggaa gaaaataaac aagttgatag actaaaaata
    22621 attaagatag tcatgggcgt gatattttta gctattttag taaaaataat ctatatgact
    22681 acatttaaat atgaatatta caatgagtta gctgagaata aaacatataa aaagttggca
    22741 atagaagctc caagaggaga aataaaagat agatatggaa gactgttggc aggaaataaa
    22801 aacttattta ctgttcaagt ttcaggtaac gatattaata agaaagatgc aaataagcat
    22861 tcaagagcaa atgaaatatc attaaaactt ataaatttgc tggagagaaa tggtgaagaa
    22921 tatgtagatg agtttcctat atatgtagaa aatggaaagt actattatac atatgacaga
    22981 gatattaggg aatataaatc agaaaatggt attccaaatg attataatgc taaggaaagt
    23041 ttttattact tggtggacaa acttatatca gctggaatat tatctcaaga agataaaaga
    23101 ttggatgcga caagattaca ggcaaaacta aatgaaaatg gatattatcc acctatttta
    23161 gttagtaaat ggatgttcac agcagaaaga gataagagag attggttggc tagttataag
    23221 ataaaagaaa ctaaactgag tgccaaagaa gcgtttgaaa aagtaagaaa tagtgatgca
    23281 ttagaaattg ataaaagttt gagtgatgaa gatgcaagaa aaataatggt cgtaagggat
    23341 ttgataaaat caaaaggata ttctcaatat aatccagtaa ctattgctaa ggatgtggga
    23401 gaaactacga ttgctcaaat tgaagaaagt gctatggacc ttgttggtgt ttcaatagca
    23461 gtagaaccag taagatatta tcctaatggg tcattagctt ctcatatgtt aggatatgta
    23521 ggaaaaatgc catccactca aatagaatca taccttcaaa aaggttatga aactggagat
    23581 atggtaggtc ttgcaggagt agaaaagagt aatgaaagta gattaagagg aactgatggt
    23641 tataaaatgg ttaaagttga tgcattgggt agaatcagta aagaaattga atctaaaaaa
    23701 cctaaatcag gagatactgt gtatcttaca ctagataaag atttacaaga agtttcagat
    23761 aatgcattaa aacagataat cgaagttgct tctaaaggag gtacttttaa aagtaaattt
    23821 ggggacaaac caattagtgc atatgcagga aaagcacagt cagcagctct tatagctatt
    23881 gatgttaaaa atggagaagt attagcttca tcaagttatc ctaattatga cccaaataaa
    23941 tttgcaaaag gtatttctac agaggattat aaagcacttc aaccaaaaaa tccaaatgac
    24001 ttactagcag gaagtccact tttaaacttg gttacacaag gggaatttca gccaggttct
    24061 tcatttaaga tgctaacttc aatggcagcg ttagaaaatg gtctggatcc aaactttact
    24121 ataaatgacc ctggggttat aatgttaggt aaaaaatctt ttggtgacta tgtatggaac
    24181 catggtagag gaaaccatgg tatgacaaac ttatataaag caatacaaga gtcttgtaat
    24241 atatatatgg caacaattgg tacaggtaaa acatggccag atggaaaaag cattggtata
    24301 gatatgaatg caaataaaat acttgagtat gcaaaattat ttgggcttga ccaaaataca
    24361 gggcttcaag atgaagtaga agaaagagca ggtaaagttc ctagcacaga agataaattg
    24421 aaatcaacac aagctcttct aaagtcaaat ttagaaagag aaatggcaaa tgattttgtt
    24481 gatataacta gagaaaaaaa tcctaaagaa tatgagaaaa gaatcaatga gattgtaagc
    24541 tgggcagcag aaaagaaaac tcctggtaga gttgaaacaa tgaataggct aaaaaaattg
    24601 gatgttaaag aagatagaat tgaagaagta gcagatttag cagtatttag ttattttaat
    24661 tttgcaaaat ggagtactgc agatacattc aacttagcta taggacaagg tgaaaatgct
    24721 tatacaccag ctcaaatatc aagatatgta gctgctatag caaatggagg taatttagta
    24781 gaattatctg tagttgatag agctgtttca agtgattatt catcagttaa aattaatgac
    24841 cagaagaaag tcgaaaaaat accttttaaa aatccagata acttaaaaga attaacaaaa
    24901 ggtatgaaat tggttgtaag acaaggtaca gcgaagagtg cttttgctga ttttccaata
    24961 gatgtagcag caaaaactgg tacagctgag aaaagtggga aaatacctac agataacgag
    25021 tatgaatatt taaaaagtca tatgtcatct tataatgtca acctaaatga tgcgattaaa
    25081 ctagctgata agatgaaagc agaaaaagaa aaagagcttt cattagctaa ggaaaaggaa
    25141 ataaaaaaga aattagaaaa taaagatttg aaagacgaag aaagaaagaa attagaggaa
    25201 gaattagaag atggagtaaa agttagatta gaggacactg ataaggtgaa ttcttcatat
    25261 ctaagaaaag ctataaaaga attaaatcca aaaataacag atgaccaaat agatagattt
    25321 aaacaagatt atggttcttt tacgtggact gttgcttttg ctccagcaga tgacccagaa
    25381 atagctgttg tatgtgtaat tcctcaaggt gattcaagtg tattctcact tttaccaact
    25441 agagaagtta ttggaactta tatgggatta gagccaacta atagtaaaaa tgacaacaag
    25501 acgaatgatg ttaacaattc ttctgacgaa aatataaatt tcgagtcaca aattaataga
    25561 tagctcaatc tatgtataaa ctttagcttt atatagaaaa aataaaaggg tttaaagcgt
    25621 atttgaagaa tataaatgat atatcactct ggaggttgtt atgtctttaa gagaaatttg
    25681 tagtcaagaa ctagtagagt tcaagggtaa taaaagaggg ataatagtaa atataaaaag
    25741 agaagctcca tttgaggaaa tacaggaaaa aataataaac aaattggaag catatgttgg
    25801 cttttttaat ggggctaaaa ttagtaaaat taatagtgat tgtttaactg acatggagat
    25861 tctagaactt aaagaaggaa ttacttctag atttgatgtg gaatttgttg aagatcaaaa
    25921 aattgaagaa aatagtaatt ttccaacaaa atatgtcaat accctaagat ctggtgaaaa
    25981 tattgaattt gaaggtgatg tagttatatt gaatgatatg aaacctggtt caaaagtcct
    26041 ttcaaaatca aatacagttg ttatgggaga tataaatgct ggtgctaagg ttgtagcagg
    26101 aggtaatgtt tttgtaatgg gtaaaataga aggttttgta catgcaggtg cagaaggcaa
    26161 tgaatttgca tatgttgttg ctggaaatct taatcctaaa atattacaaa tagcagataa
    26221 tattgcagaa gctccagatg atgaagaaaa ttatgaaagt gaatcagaaa ttagcccaga
    26281 aatagccttt gtaagtaatg gaagaattgt aatcgaaagc tatttgtcaa agttagacaa
    26341 atagaaagaa aaataggggg tagtattatg agcgaagtta tagttataac atctggtaaa
    26401 ggtggggttg gaaaaactac taccgcagca aacttaggaa ctgcgctaag cctagaaaat
    26461 aaaaaaacag tagttgtgga tgcagatatt ggacttagaa acttagatgt agtaatgggt
    26521 cttgaaaatc gaatagttta tgacatagta gatgtagttg aaggaacttg taggcttaag
    26581 caagctctaa taaaagacaa aagatttgat aatctatatt tattaccagc agcacaaact
    26641 agagataaaa atgctgtctc agttgagcag atgattgacc tgtgtgagaa actaaaagaa
    26701 tctttcgagt atataataat agattgtcca gcaggtattg agcaaggctt taaaaatgca
    26761 gtagctgggg cagatagagc tattgtagtt actaatccag aaatatcagc agtaagagat
    26821 gctgatagaa taataggtct attagaagca aatgaaataa aagagataag attagttata
    26881 aatagaatta gaaatgatat ggttaagcgt ggagacatga tggataaaca agatataata
    26941 gaaatattag caatagattt gttaggtctt gttcctgatg atgaaagcat aattatatca
    27001 acaaataaag gagagccagc tatacttgat tctaagtcac ttgctggtca agcatacaaa
    27061 aatatcgcaa aaaggatact aaatgaagaa gttcctctac ttgatcttga agttgaagat
    27121 ggattctttg gtagacttaa aaaaatgttt agcatggcta agtagcaaag gaggatacat
    27181 ctgtgttaga tttatttaga gttttttcta acgaggctaa aactagtaaa tctgttgcta
    27241 aagagaggtt aaagctagtt ttagttcatg atagagtaga ttgttcacca caacttttag
    27301 agatgataaa aacagacatt ttaaaagtaa tagctaatta tgcagaaata gaagatgatg
    27361 gtcttgaaat aaaaatgtct aaatgcagag gtgagcatga tgacaaacct gtatctgcat
    27421 tagttgctaa tataccacta aagaatataa aagatagatg tatgtaatta gtgcttaaaa
    27481 gggggatatc cttattggta tatgcccctt ttatttaatt atatgtttaa agatagaaag
    27541 acggtgattc tttgaaaaaa atcaatatta attataagtc gacaattaag ttgataaaac
    27601 agttagactg gaaacttata gttacagtat tagctatctt tatttttggg cttgttatac
    27661 ttagtagtgc aacacatgct aattcaacag gttcatataa tcaattgatt aaacaaggat
    27721 tagcttttgt acttgggata ggaatgatta tagtcatatt attttttgac tataaccttc
    27781 taggcaggta ttataaagcg ctttatataa taagtcttat tttgttagcc atagttttat
    27841 tacctggaat tgggacagta aaaggtggag ctaggtcgtg gattaactta ggaccacttg
    27901 acttgcagac atctgaaata gtcaaattaa catttgtact aagttatgca aagatacttg
    27961 aatccaaaaa ggataaatta aatacgttaa aggaagttat gcctgttgta gtttattctc
    28021 ttccatttat cggattattg attgcacaac cagacttggg aacaggaatt gtattttgtt
    28081 gtatgatatt tgcaatgctg ttcacagcag gtcttagctc aaagttgata aaaagaggaa
    28141 taatcatact attagtgtca atgccactga tgtatttaat gatggcagac catcaaaagg
    28201 taagaataga agcttttcta aatccagaag atgtaactct aaaaggaaac tatcaagtta
    28261 tgcaatctct gattgcaatt ggttctggag gcgtaactgg taaaggactt tacaatggaa
    28321 gtcaaaatca agaagatttt ttacctgtac aagatagtga ttttatattt gctgttgtag
    28381 gtgaagaact tggtgtaata ggaatggcag tgttgataat cttatttatg atattcctat
    28441 taaggctttt agcaattgca agagatgcaa aagattttta tggaaccctc atagtggttg
    28501 gtgttatggg aatgtttggt taccagataa tccaaaatat aggtatgacg gttgcgttaa
    28561 taccagtaac tggagtaaca ttaccttttg taagttatgg aggaagttcg ttattgacat
    28621 ctttagcaaa cttaggatta gtactaaatg tgtgtatgag aagaaagaaa ataaatttct
    28681 aaatttaaat acatctaaac ataaattgag gagattagat atatgaatat agcattagta
    28741 gcacatgacc aaatgaaaaa cactatggtc ggattttgta taggttatga atcaatttta
    28801 aaaaagtatg gactatatgc aactggaact acaggaaaaa gaataatgga tgaaactgaa
    28861 ttaaatataa atagattagc atctggtcct ttaggtggag accaacaaat aggttcttta
    28921 atagtaacac aagaaataga tttagttatt tttttaagag acccattgac atctcaggct
    28981 catgaaacag atatacaagc gttaataaga ctttgtgatg tttatcatgt tccaatagct
    29041 acaaatctag cttcagcaga aatatttata aaagctcttg atagaggaga actatcatgg
    29101 agagaagtta gaaaaagcaa aagtcaacgt atttaaaaaa tattaatatt acagtctaaa
    29161 gaaacagata tataagtgtt taatgcccta gtctttctgt tggaataaat ctagtacctg
    29221 tagaaatctt atgaaagatt taaatgatga agagctaaat ttgagattaa ataattagga
    29281 aatagattat agatagagta gtagaattta aactattact ctattttaat aatatgaaat
    29341 agaatcaagt ctattattat atatatttaa acttataggt aatactattt caaccaagta
    29401 aatgttgtta tgacaacatt aataaaacaa aaatttattt ttaaaatttg ataaatagct
    29461 gtatattagt atttatagat taaatattaa tatacagcta tttattattt ttaagtctat
    29521 tgtagaatat aaaccttgca atatagagta ctatgtaata ttatagtaaa tataagtact
    29581 atacattata gagtagtagg tgaaaaggcg gtggtacata atgcagttaa ataaagaagt
    29641 gttaaaaggt tatatcgata tattaattgt ttctatatta gaaaagaaag attgttatgg
    29701 atatgaaatt gcaaagcaag taagagaaag aagtgagttt gaattaaaag aaggaacaat
    29761 gtatcttgca cttaaaagaa tggaatctaa aaatttaatt aaatcttatt atagcaatga
    29821 acaaagttca ggtggaagaa gaaaatatta taatttaact aatgaaggta aagatttttt
    29881 agaaataaag aaacaagaat ggagatttat taaaaaggtt atgaatcaat ttttgggaga
    29941 ggtggattaa tttatgaaac taatagatga atatttagat aaactatata aaaaatgtga
    30001 caataaaagt actatagaat taaaacagga aatgagatgc catcttatag aatctgcaaa
    30061 tgaatttaaa ttagaaggat tagatgaaga agaagcatgt aaaaaagcaa ttgaaagatt
    30121 tgatgatggt gatgaaatgc agtatgaatt atgtaatata ataaaagaac tatctttatc
    30181 attggatagg cataaatcaa ttgtaatggg gtttaaaaaa gtattaggtt acataagtat
    30241 aattgcattt ttaatttctg gatttatgtg gtattacaat aatagtttac aacataatat
    30301 gtataattta ggaaaagaac ttgatggaga aataaagcaa ttggcagaaa gacatgatat
    30361 gacaaagatt ggtgaatata agttagaact tgaaaaaata cttgataaag ataagtatag
    30421 taaagtcaaa gctcttagac tatatgtaat agatatgaaa gatggaaaca ctaatttaag
    30481 ttcttctgga ttgaatgcta atatggtata tgagagggaa gcagattata ataacataag
    30541 taattttata cagcatttag gatataatgg aaaagatttt ttagacaaga atgggaatat
    30601 agtaaatcca gatattttct tagaatattt cttttatttt gaatcggaga tgttaattcc
    30661 agtagcattt gcatttggat tattatgtat aatagcgtat tttatattaa gatttaaaat
    30721 ttctttaata aaaaataata attaaataat tcaccaaata atcttaattt gtagagtaaa
    30781 taagatgttt caaaataaaa tttgaatcat cttatttttt attgtttgtt atactctttt
    30841 cttttatatt tctccatagt ggattgtatc tatacataat cacatatttt gatataatta
    30901 tactttccaa aaaatgtatt gaatatacaa attataatct gtataatgtt gttaggggag
    30961 aatgtatgaa gtgtatacaa ttttaatttt aaatgtatta ttgacaattc tcctcactct
    31021 cgccattacc aataattgga aaattgacat acctgttaca gaccaacatt tttgaatata
    31081 ttaattatta tcattttatt gtgatgatat caaaaatgca ggtttgtgag ttggattgta
    31141 tacgaactat ataggaaata tagaacagat tttccaagaa atctaaatat tctatgacca
    31201 cgtgaattgc tatgcataca tgttgataat ccatatttta aatctaaaat gaacttaatt
    31261 aatggtaggg aattgaaagt aaaatatatt ataaaggaga atgctaatat gaaaaaaatc
    31321 aggaaattag ctactaaact attgattgca atgatggttt taacctcatg tatgagtgta
    31381 acagtttatg gtatgacagc taaagatgtt actgcaaaga caccgaaaag ttatgtagct
    31441 ggaaccaaca gtatatatgg tccaaagtta tcacaagctc aacttaattc ggtagcacaa
    31501 gcaacagcag actttatgaa caagaaaatt acaaagaaca tgaccacaga tgctaagatt
    31561 agggttgctt ataatcacat taaaaacaac acaacatatg ttaactggaa cgcagcagag
    31621 ggagccaata ctgcttatac attggttact aaaaaaggtg cttgttctgg aatgtgtagg
    31681 agcttgaaag ctttatgtga tgctatggga attgaatgtt attatgtaca tagtacaaca
    31741 aatgaccatc agtggaatct tattcgtttt gaggatggac gtttgtatca tgtagatatt
    31801 gatgccaaca aatcagcagg agcagacatc atatacaaga gtcttactca cccattacca
    31861 tttgacaaga aagcatatcc agctgttggc gaagtagtaa agacacagaa tccaacgcca
    31921 actgttaaga aacctacgcc agctaagcct tctgcgccag ttggacctgg gaatcacact
    31981 atgatagcag atcaacaagg ggcaatactt gatgaagttt atggaaataa tctacgtcca
    32041 aaaattgaaa tgtatgaggg agttccgtgt agtttaatca actcatcaat aaatacaatt
    32101 aagtttacaa gcaaaaattc aaatttagac ccatcaaagc atacagttac tgtaataggg
    32161 tttatgaata caccatatgg tatggttgca ttagctccag atggtgtacc tgatgagtat
    32221 ccttttgaat atgataagcc attttcttta gattttactg gtaagaacct ttctgcagca
    32281 gaattatcaa aaaagtgtaa agaaaataat gtaattatta aagtggaaat tggtgaaacg
    32341 gcatctaatt ctggaagagg gatggtagca tacgctagat atgtgaatta atacaaatat
    32401 actagtgtac aaatagaaca tattataaat aatgcaccct cgcacaaagg gtgcatttat
    32461 cattatatca gtcattttag aaaataaaaa aggagttgaa cttaaaatta gattagaaaa
    32521 attaattttg agacaactcc attttaattt atttttaaat ataaaatcat ttattttaaa
    32581 taatattatg tttgattaat ggctatttaa ctaattttgc aaattctgca ccaaatgcca
    32641 tacactcttt taatgtctct tcactaggga agaatttttt cttaagagat tctacaggca
    32701 ttttgaatga catagattta agtaaagtct cagccatagt tataccttca ccactccaac
    32761 caaatgaacc aaatactcct gctatcttgc cttggttagc cattgggtct ataacagaga
    32821 ataaatccca cataggttta accatagttt tgtttatagt tggagaacct agtaatatta
    32881 cttttgacac tactaatgta tcgtgcattt ctgttaatgt catatcttct aaatcatata
    32941 attcagcctt tgcaccttct ttatcaagtc cttcttttat ctttttagcc atctctaaag
    33001 tattactata agctgataag tagaatatag aaacttgatt ttgattagtt gtattaacag
    33061 cttctgtaga ccactcaaca tatcttttaa cagcagccat tgggtcttta gtaagcattg
    33121 gaccatgaga agtcaatata gtatcaaatt ctatgtttag tcctaccacc ttatctacag
    33181 cacttaaaac atgtttagca aatggcttaa ctatacaatc atagtagtgt ttagctgatt
    33241 ttagataatc ttcactattc actacctctg catcaacact tgcaaagtga caaccaaatg
    33301 catcacaagt taaaagagtc ttgtcctcct caatgtaagt aaacattgta tcaacccaat
    33361 gtaagaatgg agcagttata aatctaagat ttcttttacc gatatttaaa atttcaccat
    33421 cttttattac atgacagtta aaaggtctat ttatttgacc atctaagtat agtttagcag
    33481 ctttagtaca ataaacttct atattaggat ttatatctaa taagtatttt aaactacctg
    33541 catggtctgg ttctgtgtga tgaattacaa catagtcaat tttagctata tcagtaacct
    33601 ctgataaatt acttaaaaac tcatctttaa aatttgcctt tacagtgtca aataaaaccg
    33661 ttttttcgtc cttgattaaa taagagttgt aagttgtccc aaactcagtt tccattatta
    33721 tgtcgaatac ttttaaacct tcatctacta ctccagtaaa gtagatatct ttttttactt
    33781 caaaaacttt actcattttt aaccctccta aaatttatca ttatataagt aacatacccc
    33841 attttgaggt gaatgaaaca aaagatgtca tgataagtca tgtaaaacaa tagttttatg
    33901 tttataatat atcatacttt gtaaaaaaat tgtatttcaa taaaaattta atagaaaata
    33961 ttcttaaata tttttagcta ttctattttg tgaataatat gtattttaaa ttcaatattt
    34021 tagttatatt tttgatagaa aatattttat cgtgtataat attacttata aataagaata
    34081 aaaataaaga gcttgtttgg aggaatttta ataaatgaat aaagtagact tgaaaaaaat
    34141 cttaaaaaaa gtagaaaagc ctgctagata tcttggaaat gaaataaatt caattcataa
    34201 agatactagt aatagtgaat tgataagata tgcacattgc tttccagatt tatatgaagt
    34261 tggtatgagt cacttaggga gtcatatatt atatgatgta ataaataagg atgaagatgt
    34321 attttgtgag agagtgtatt ctcctgcagt tgatatggaa aatataatga gagaaaaaag
    34381 tataccatta tttgcattag agtcaagaga gcctataaca aactttgatt ttgtcacttt
    34441 tacacttcaa tatgaacttt catacactaa tatcttaaat atattagatt tagccaatat
    34501 accaatatta aaagaagaaa gaactttgga agacccattt ataatggttg gtggtccttg
    34561 tgcttataat tccgaaccat tagcagattt tgtagatgta gtaatattag gtgaaggtga
    34621 agaggttaat ttagaagtag taaatgagta taaagaatgg aagaaaaata aaacgactag
    34681 agaagatttt ttatataaaa tatctagcat agaaggagta tacataccaa gtttttatga
    34741 tgtcaagtac aatgaagatg gtacagttca aagtgtaact cctaatagag aagggattac
    34801 taaaaatcct actaaaagga taataaaaga tgttgagact gtagattatc cagaaaaact
    34861 aatagtacct tatatagata ctgttcatga tagaatagta ctagaattat ttagaggatg
    34921 tactagagga tgtagattct gtcaagcagg tatgatatac agaccaataa gagaaaagag
    34981 tgttaaaaga cttaaagaaa tattagataa attagttaag aatactggat atgatgaaat
    35041 atcattatca tcacttagta cgagtgatta tagtaagtta tcagagttga ctgattattt
    35101 ggtagatgaa tatgcatcta ataatattgg gatttcgctt ccatccctta gattagataa
    35161 tttttcaatg gaaattgctg ataaaataca acaagtaaga aaatctggac ttacttttgc
    35221 tccagaagca ggaactcaaa gacttagaga tgttataaat aaaggtgtta cagaagaaga
    35281 tttggaaaat gcaacagaaa gagcttttga gatggggtgg aatagtgtta agttgtattt
    35341 tatgattggt ttaccaactg agacttatga agatttagat ggtatagcta aattagctta
    35401 taaagttatt gatatttata gaaaagtaaa tggaggaaag ctaaagagaa gttttagtgt
    35461 tacagtgagt acttctacat ttgtaccaaa gccatttaca cctttccaat ggcatggtca
    35521 agatactaca gaagaagtta ttaataagca gagacattta gtaaataaat taagaaataa
    35581 caatataaaa tacaattatc atgattcaaa gactagtttg atggaagcag tagtagctag
    35641 aggcgataga agaataggaa aagttatata tgatgcattt agactaggtg ctaaatttga
    35701 tggatgggca gaacatttta atttagatat atggaaagaa gctatggaaa agaataatct
    35761 ttctatagat ttttatgcac atagaaatag aaactatgaa gaggtatttc cttgggacca
    35821 tatagatgta ggtattagta aaaaattctt aattagagaa gacgaaaatg ctaaaaaaga
    35881 aaaaatcact tctgattgta gacataactg caatggttgt ggaataaata tacatgatat
    35941 aggaaggggg ctttgctaat atgagtaaga taataagagt aaaatttaaa aaggaaggcg
    36001 acatgatata tatatcacat cttgaccttc agagattgtt acaaagagcg tttagaagag
    36061 ctgaaataaa cttatcacat tctcaaggat ttaatcctca ccctaaaatg agttatggca
    36121 atgctttggc tttagggaca gaaagtcaag gtgagtatgt agatattgaa atagaagaag
    36181 atgatttatc agtagaggaa tttttaaata aagtatcaat tcaacttcca gatgggatag
    36241 attttatcaa agcaaaagaa attgatagac aaacaccttc tctttcttct gtgatagact
    36301 atggagaata tttgtttaat atagatttga aaagacctat gacgaaagag tttgtaaaaa
    36361 gaaaagtaat tgactttatg aataataaag aaataatcat aacaaaaaag aataaaaaag
    36421 gtaagatggt tgaagtagat ataagaccta tgataagaac ctttgatgtt ttaaatttag
    36481 aagatgaaca tattactctt actgctacta tagctacagg ctcaaaaact aatttaaata
    36541 ctaatatact tataccaaag atattagaga tgtttgaact tgatattgac ccattagatg
    36601 ttgatatatt gagaagagat ttatatgtat tagaagatgg ggaattagtg actcctatgt
    36661 aagctcgaga ggtgaagttg tgaaaaagat agttattgag tccctaatag ggtcccagaa
    36721 aacagcagta cttgaagatg aaagattaac agaacttttt gttgaagaca atttaaataa
    36781 gaagactgta tccaatatat atcgtggaat cgtaaaaaaa gttataccag gaatagaagc
    36841 ttgttttgta gatattggat ttaagaaact tgcttattta caattaaaaa agggaagtac
    36901 tataaaatct ggtcaggata ttttggttca gataaataaa gaagaaatag gaactaaagg
    36961 cgcaaaatta aacactgaga ttagtatttc tggaaggtat attgtctata taccttccaa
    37021 tgatagaact acaatatcaa ataaaattac tgatgaaaaa gaacgattta gattaaaaaa
    37081 aattactaag gcagtaaata aggaaaatct aggattaata ataaggactg aggctcaagg
    37141 ttgcactcat gatgaaataa agaaagatat agaagagttg aaattaaagt atgaaaacat
    37201 tttaaaagaa tataaacttg gtataggtcc gaaattatta tataagtctt tagattttgc
    37261 aactaaatat gtaaaagata atgtgaatga tgatattgaa tctataatta ccaatagtta
    37321 tgataagtac tctgaactta agtctatttt aagaggtata gataagactt atgttgataa
    37381 actgtgtctt gaggaaaata gagatgtatt tgatttatac agaatagaat ctaaaataga
    37441 aaaattatta aataaaaaag tatggttaaa gagtggaggt tatttaataa ttgaaaaaac
    37501 agaagctctt acggttatag atgtaaatac gggtaagttt attggaactg gtaaacttga
    37561 tgaaaccgtt tataaaacta atcttgaagc tgcaaaagaa attgtaagac agcttagaat
    37621 tagagacata gctgggataa taataatcga ttttattgat atgcataaaa agaagcatca
    37681 aaaggagatt ttacacatcc tagaagaaga atttaataaa gataaaagaa aagctgaagt
    37741 attaggtatg acaaaacttg gtttagtaga agttgctagg cgtagagaaa aagaatccat
    37801 tgataagtat tatttaatgt cttgtccatg ttgtgatggt gaacaaacaa taaaatctgt
    37861 acattatata ctagatagta ttgaaaaaga aattatgaga ataagtgaac atactgttta
    37921 caaaaacata atggtagaat ttaatgattt tatttttgaa caaataaaag aatattatat
    37981 ggatataatt gataaaattg gtgaaaaata taatataaag atttctttaa acgcaaatag
    38041 tacattaaag cacaataaaa ccaatgttat tttcgataaa atagttgaca ataaaatgta
    38101 aatattttat aattaataat tgtaatgccg cacagtaaag gtttaaatct tcaaatgaag
    38161 taccttgtac tggcgagttt gaggccgagg aggtgtattt aatgtacgct atagtaaaga
    38221 ctggtggaaa gcaatataaa gtttctgaag gtgatgtatt attcgttgaa aaattagaag
    38281 ctaacgcagg tgatgttgtt actttaaacg aagtattagc ttgctctaaa gatggagaat
    38341 taaaattagg ttctccagta gtagaaggag catctgttca agctaaagtt gttgaacaag
    38401 gtaaagctaa gaaagttata gttttcaagt ataaagcaaa aaaagattac agaagaaaac
    38461 aaggacatcg tcaatcatac actaagatag ttgtagaaaa aataaacgct tagttattgg
    38521 ttaagttatg ataaaagtta aatattatta taatgatgat ttcttgttgc aaggtttttg
    38581 tttaaaggga catgctgatt ttgctgaaat aggctatgat atagtatgtg ctgcagttac
    38641 atctaatgct attgcagtta taaattcttt agacaaatta caaaagattg aatttgaaaa
    38701 agtagtagga gaagaaggtc atatagagtg tatagtcaaa gatacacatg taaaagacgc
    38761 acaattatta ctaaatcact ttcaattagc ggttaaagaa attaaacggg aatacccaaa
    38821 aaatataaaa attttaaaaa agtagggggt gactccatat gttaaacatg aatttacaat
    38881 tattagcatc taaaaaaggg gtaggttctt ctaaaaatgg tagagattct atatctaaaa
    38941 gattaggtgt taagagattc gatggtcaat tagtaactgc tggaagtata atagttagac
    39001 aaagaggaac taaaatacat ccaggaacta atgttggaaa aggtagtgat gatactttat
    39061 ttgcattagt tgatggtact gttaaattcg aaagaaaaga caagaagaga aaaaaagtta
    39121 gtatatatcc agtagctata gctgagtaat atacataaaa ggagtgttga ggcactcctt
    39181 tttcttataa tactgatgaa tttataatgg tatttttggt aatgctaatt aaaagttttt
    39241 aattaagtag gtgaagaatt tgtttataga taaggcaaga atatttgtta aggctggaaa
    39301 tggtggaaat ggttcggtag catttagaag agaaaaatac gttccagctg gtggtccaga
    39361 cggtggagat ggtggtcgtg gagcaagcat catatttgaa gttgatttgg ggcttagaac
    39421 tctaatggac tttaagtacc agaaaaaata tcaagctcaa aatggtggcg acggaagcaa
    39481 aggaaaaaga gctggtaaaa atggagaaaa tttagtatta aaagtaccag caggtacagt
    39541 aataagagat gaggctacag gtcttgtatt agcagattta aaaaaagaag gagatacagc
    39601 tatagttgca aaaggtggta taggtggtaa aggtaaccaa cactttgcaa atgcagtaag
    39661 acaagctcca gcttttgcaa aatctggtac agatggagaa gaaagatgga taactcttga
    39721 acttaaaatg atagcagatg ttggtctttt aggatttcca aatgtaggta aatcaacatt
    39781 tctatcagtt gtaactaaag ctaaacccaa aattgcaaac tatcatttta caacacttac
    39841 accaaatcta ggtgttgttc aaactaagtt tggagatagt tttgtacttg cagatatacc
    39901 tggaattata gagggtgctt ctgaaggcat aggattaggt catgagttct taagacatgt
    39961 tgagagaaca aaggtattga tacatattgt agatatatct ggattagagg gaagagaccc
    40021 tattgaagat tttgacaaga taaatgatga gcttaaattg tacaatgaaa agctttcaaa
    40081 gagaccacaa gttgtagttg caaataaatt tgatatatta gaagatgaga gtaagtttga
    40141 gaagtttaag agtgaattag aaggcagagg atatacagta tttaaaatgt cagcagcaac
    40201 tcgtcaaggc atagatgaag taatagccta tgtttctaaa atgctaaaag aagtagaaga
    40261 tgtggagcta gtttcagaag aagaaatgta tagaccagaa cttgatattg gtactgaaga
    40321 agagttaagt atagatattg aagatggtgt gtatgtagtt acaggtaaag cacttagaag
    40381 aataatgtat tctgtaaatt ttgatgatat ggaatccctt caatatttcc aaaaagctat
    40441 ggagagtcaa ggtgtttttg ataggcttag agaaatggga atagaagatg gagatgtggt
    40501 taaaatttat gaattagaat ttgagtttta caattaattt aaatatagat tatagataat
    40561 ttaagtatag aattatagag gtgagaatat gttaaaagga aaacaaaggg cgtatttaag
    40621 atctttggca aatactttaa agcctactac acaaattggt aaagaaggtg ttacagagag
    40681 tttcttagag caattagatg gtatgttaag aactagagaa ttagtaaaag ttactatatt
    40741 agaaaatgca ggtcttgata ctaaagaaac tgctaatgca gtatgtgaag ctttaagagc
    40801 tgaatttgtg caggctattg gttttaaatt tactatttac aagaggaata ttgaagagcc
    40861 taaaatactt tttccaggac atgaacaggc gaaagcaaag actaaaaata ataatgttac
    40921 aaaaaagggt aaaccaacta aaagagctgt tagataatag tttatatcct tttagggaaa
    40981 taagttaaaa aaatagtgac aattggaaga aattgtatta tactattaaa cataaattta
    41041 aattagcaat cttatcaaga gtggcagagg gactggccct gtgaagcccg gcaaccagta
    41101 tatttaatta tacattggtg ctaaatcctg cagcatttat gctgaaagat gagtataaac
    41161 ccgtcatatg gcttgagttt atactcaagc tttttttatt ggtatattta tatatataaa
    41221 taatttaata taatcaaaat gatgggggta tgttacagtg aagatagcaa tattagggta
    41281 tggaacagta ggaagtggat taattgattt aatagaaaat aatacttcaa aaagagatat
    41341 agaagttgtt ggaattttag ttaaaaataa agaaaaacac aagtataaga agtactttga
    41401 taagataact actgatatag aagatatatt tgacaaggat attgatatac tagttgaagt
    41461 aattggagga ttaaatccag cttttgatta tgtaacaaga gcgttaaata aaaaaataca
    41521 tgtagtgaca gctaataaag atttactggc tgaaaaaggt agtgacctta tagaattagc
    41581 taacttaaat aatgttagca tcaagtttga agcatctgtg gcaggtggta ttccagtttt
    41641 aaaaccattg atagaatcac tggaaggcaa caatataaag agcataaacg ctattttaaa
    41701 tgggacttgt aattttatat tatcaaagat gtatgatgaa aatttaccgt atgatgtcgc
    41761 tttaaaacag gcacaagagt tgggatttgc agaagttaat ccagatgcag atgttttagg
    41821 atatgatagt gctagaaagt tatctatatt gtctacttta tcttatggaa aaatagtata
    41881 ttggaaagat ttattattag aaggaataac ttcaatagat gaaaaagata tagaatatgc
    41941 aaaaaaatta aattgtaaga taaaattggt tgctagaagt aagtatgaat caggagaagt
    42001 aagtggattt gttagaccag cattagtgga caataataat atgctttcaa agatagataa
    42061 tgagttcaac gtagttattt tagtaggtga ttcagtaggt gaactttctt ttgtaggtaa
    42121 gggagctgga agaggtgcaa caggaagtgc tgtttatgca gatattatag atataataga
    42181 taatagaagt tcaaatataa agtctttttc aaaagggaaa ttggatttaa gtggacttat
    42241 agaagatgag tgtagtgcag tagttagatt tggtgggtat aataataaag atggaatttt
    42301 agaatgttta gataaatatg tagatagtta tgatattata gatgatgaag aattggcgat
    42361 ttttgtaagt gcaaagtcag aacatgagat tgatagagtt ttggaaattg taaaaagaaa
    42421 taattacagt gaatcagcta aaaaattatt gaagatagat taatatgttt atgcaagaat
    42481 tatgaaaatt taatagggtg tctcaaaata gagagataag ccttttttcc agtgttgaaa
    42541 taataaaaat atatttgatt ttataaaaaa agagtgtctt atgaactaaa aagttcattt
    42601 tgagacactc ttttttaata tctggtgaat taataagata atagaaaaat gtatacgtta
    42661 taattaacca ataaagaaat tttcactagc ttcattaata gcttgctgag ttacccttat
    42721 atatcgctgt gtagtggcta cagagctgtg atttaaaagt tctttaacca actctaaatt
    42781 attgttactc ttttcaaatt gagtagtagc atagagcttt ctaaagctat gtgtacttat
    42841 attatacaga tttaagtgtt tacaaattat cctcaactgt ttttgaatag cctttgtctt
    42901 tatatttact aagtagtcat ttgatttaag attatacttt cttgcatgtt catttatgga
    42961 ctcaattata tttttattaa ctggtctata ctgtaatttg cccgtctttt tttcatttat
    43021 ttccaacata ttacctttaa aattacctat ctttaatctt aaaatatctg atattcttag
    43081 acctaaatta gcttctaaca ttagtgccaa tgcaacttta ggattttttc taaatctttt
    43141 ttcaacgcca ttttcactat aagtaaagcc agtatgtaat agttccatta tctttttata
    43201 ttcgtctatt tctataggtc ttgttgccat tttcaaccac tcctcttagt ttgatttggt
    43261 aagtttaggt cggtatttat tatataatat aaatgaaata ttatataatg gaaaaatttc
    43321 ccagtaaact tatacaagtg tgatatatgt gattaaggag aattttttga aaaatatttc
    43381 taataaaatt attaaattgt gatgaaataa ctatgctgtt aaactaatta aaaaggttat
    43441 taaaattatg acaaatataa taaaaaaatg gaagtattta gttaagtttt attagtttta
    43501 tttttagttt tatttttttc gtgaaaattt cttagtgaaa aaagtatttt tatatcgcat
    43561 tagtttccca tttgtagata aagttttcat atgttttttt gcaataaata cggctgagat
    43621 tagataaatt attactacaa tttgaagaat tgtatcataa tagctaaggt aataaaacca
    43681 gattaaatta ttatatataa cgttgtatgt atcagcaaag ctagaaacca acacagcaga
    43741 gtttgcaatt ggataaagta taaatataaa gatagcagct aaaactccat gcgcaaattt
    43801 acttagtatg tataatttta aatctatgtc cgtttttgat agaaatgtac aacattgagc
    43861 taggatggat aatccactaa atcctatcaa aaaactagct aaagatgctc ttaaaatttc
    43921 tggagaactt gttatactag atactttatt gcatcctata gtaatttcaa atagaccact
    43981 tacaaatgct tgacagagtt ctttactaac acctaaaaat gaaagaggca aatatataac
    44041 tgatgctatt aatgagatta cattaaaaag agatagtatt ttaaatacta ctgaaaaaac
    44101 aattacaaat ccaccaactg taagtaatgt gtttactcca ttaaatacag catttccaaa
    44161 taaaacaaaa aaaccattcc cacttgaatt tctaatgttg attacatttt ttatatttgt
    44221 ttttaaatcc aattttgtct ttggaaggtt ttcattacca taatttctaa agaaaagacc
    44281 aaccaatata gttcctagat aatggcagag aatcattaaa taacctaagt ttgaattttg
    44341 gaacatacct gtaccaaccg aacctattat gaataaaggt cctgatgtag aacaaaaaga
    44401 cacaagtctt tgtgcttcat acttagaaat ttgtccatta ctcctaaatt cagaagctaa
    44461 acttactcct actggataac cagatactgt agatatagca aaagctaaag cgctctttcc
    44521 tgaaacatta aatacaaatc tagttatggg attgatgata aaccctataa tatccactat
    44581 ttttaaatca acaattaagt ttgctccaat tataaatggt agtagtgatg gaactaaaac
    44641 attagaccat atagaaaagc cttctcttgc agattttata gcttcatttg ggtataggat
    44701 aatccctaaa actagaactc caactatgat agatggagca aaaaaagcaa taattttatt
    44761 ttttttcata atatcacctg taaatttgag ttataagata ttatattaaa agtaataaaa
    44821 atatatgaat gtaatggctt aagatatata atagatatat attacaacaa cgattatcat
    44881 attggaggaa aaaatgttta atagtatagc tagtaaaaaa gtttatgaac aagtaattga
    44941 gcaaatacaa tataaaatac taaatggaga gttaaaaaag ggggataaat tactatcaga
    45001 aagagaactg tctgaacaaa tgaatgtgag tagaacttcc ataagggaag ctattagggt
    45061 attagagact atgggagtga tagaaagcag acagggtgaa ggaaatttta tatgtacaaa
    45121 tatagaaaaa actttaatag aacctttatc tatgattttt aaacttaata atggtacact
    45181 tgaagatata ttagagctta gaataatcct agaaattgag attgcaaaac ttgcttcaaa
    45241 gagaataact tcttctgaag ttatagaatt gaaacatata atagatgaaa tgagggtaga
    45301 aactaataaa aaagataata atcgagtact ggtattatta gaccaaaaat ttcacagtaa
    45361 actagcaaca ttaagtaaaa attatttaat tcaaagtctt tttatgacag catcaaaatt
    45421 atttgatgga tttatagaag atgcaagaga aaaaataatc gcagaacctt ttaatgaaaa
    45481 tatattgctt aaacagcatg aagctatata caatgcagtt gtagaaaatg atgtagaact
    45541 tgcatgtgag aaagcaaagg aacatatgga ttttataagt aaaaattata gaaaaaatga
    45601 aaattaaaag tttgttataa aataaattat tcttgcaaat ggtagataat ggtactatta
    45661 tataaatgta aaactggtat gaccacatac caattttata gtttattata atgggagatt
    45721 aaaaatggta aagttaaaag taccgtattc aaaacaagga atgattttag aaatacctga
    45781 tgaacgtttt ctcggtgttt tggagtcaaa atcggataat tatatagaac ataagactca
    45841 agaagagata gttgaagaat ctatgaataa tcctataggc tctaattctc tggaagaact
    45901 ggttaagggg aaaaagaatg tagtcataat tacaagtgac catacaagac ctgtaccaag
    45961 tagaataact atgccaatta tattaagaag gattaggaat gttaatccag aaattgatgt
    46021 gaagattata gtggcaactg gatttcatag gccaagtaca agggaagagc taatttataa
    46081 aatgggagaa gaaatagtag ataatgaaga tatagttatg catatatcta ctgatgataa
    46141 atctatgtgt aaagtaggga tattgccttc aggtggagat ttatatataa acaaactagc
    46201 atatgaggca gaacttttga tagcagaagg ttttatagaa cctcatttct ttgcaggttt
    46261 ttcaggtggc agaaaaagcg tacttccagg aatcgcatct gcaaagacta taatgtacaa
    46321 tcactgtagt gactttatag atagtgataa ttcaagaact ggaaaattga ataataatcc
    46381 tattcatgag gatatggtat atgcagctaa agttgcaaaa ttagcattca tattgaatgt
    46441 tgtaatagat aaagataaaa aaataattgc ttcctttgct ggtgatgtcg aaaaagcaca
    46501 tactaaagga tgtagatttg taacagagtt atcaaaagta aattctataa aatctgatat
    46561 tgttgtaact acaaatggtg gatatccact agaccaaaat atttatcaat ctgttaaagg
    46621 aatgacagca gctgaagcta cttgtaaaga cggtggagta ataataatga tttcagcatg
    46681 taatgatggt catggaggtc aatcttttta tgaaaatgta gcaaatgcag attctccaaa
    46741 acaactttta gataaaatta gaagtgtcga tagagtagat acgattccag accaatggga
    46801 atttcagata ctagcgagaa tattatctaa atatactgtg ataatggtaa cagataagtg
    46861 taatcctgaa atgataaaaa aaatgcatat gaaacatgca tttaacttta aagaagcttt
    46921 agaaatggcg aatagtatag ttggtaagga gagcaaagta gtagttatac ctgatggagt
    46981 atcagttata gttaaataaa aaaggagtgt actttatgga aattttgaca tgtattaaac
    47041 aagttcctgg aacgacttca gtagaagttg atgaaactac aggagtacta aaaagagatg
    47101 gagtagattc aaaaatgaat ccctatgacc tatatgcttt agaaacagct cttagaataa
    47161 aggaagataa gaaagcgaat ctaaaagtac ttagcatggg tcctcctcag gcaaagaagg
    47221 taattgaaga aagttttatg atgggagctg atgaaggagc attaataagt gataggagat
    47281 ttggaggagc agatgtattg gctacttcct ataccatatc acaaggtatt aagaagatgg
    47341 gaaaggtaga cctaataatt tgtggaaaac aaactactga tggtgataca gcacaggtgg
    47401 gacctgaagt agctgaattt ttagatatac ctcatgttac taacgttaca aaattaattg
    47461 aagttaaaga tgaaagtata gttgttgaaa tagatatgcc aaatgattta caagtatgtg
    47521 agattgaata tccatgttta ataactgtag aaaaagacat ttttcaacca agactacctt
    47581 catttaaatt aaaacttaat actaaagata gagaaatacc tgtatatagc ttagatgact
    47641 ttgaagataa aaatgaaaat aactatggac ttaatggttc tcctactcaa gttgtaagaa
    47701 tatttcctcc aaagccaaat acagataaaa atattgtaag aggaaatgca gatgaactaa
    47761 gttttgcact tgtaaataaa ttagaggaat taaagttggt gtaaggggga gtaataaatg
    47821 tcaaagatag ttgtaaatca agataaaata actgacttaa agagaatttt agaaatatgt
    47881 ccttttgggg ctatagaaga aaagtcaggt atagtagaaa taagtgctgg ctgtaagatg
    47941 tgcaagttgt gtgtcaaaag tggaccaaaa ggcgcatttg aatttataga gagtagtaaa
    48001 gtacaaatta ataaagatga atggagggga attgcagtat atgttgaaca tcataatgga
    48061 aatatacatc ctgtaacgta cgagcttata ggtaaagctc gtgagatggc atcaaaaata
    48121 aaacaacctg tgtattgtgt atttgtagga aaagacataa aagataagtg cagtaatttg
    48181 atttcttatg gggtggatga agtatttgta tatgatgaag atgaatttaa agattttagg
    48241 atagaaccat attcaaaagc aatagaaaac tttatagata aaataaaacc aacgatagta
    48301 ttagttggtg gaactacatt aggtaggtca ttagctccaa gacttgccgc tagatttaga
    48361 acaggtctta cagcagattg tacaatactt gatattcaat caaatactga tttagaccaa
    48421 ataagaccag catttggtgg aaatataatg gctcatataa acacacctaa taatagacca
    48481 caatttgcca ctgtaagata taaaatattt tcagcaccag aaaaaataga gaatactaca
    48541 ggaaagataa ctttatgtaa agtagaaaaa aaagaactaa aatctaaaat taaggtactg
    48601 agtgtaaaag aaaaaaataa agaagttggt ctggaagaag ctgaagtgat agttgtggca
    48661 agtagagcaa taaaaaaaca agaagatatg gagatgatat ataaattagc tgataagtta
    48721 aacgctcaag tggcaggaac aagacctgta atagaagcag gttggataga tgctaaaaaa
    48781 caaataggtc ttagtggtag aactgtaaaa cctaaattga taataacttg tggtgtatct
    48841 ggagctgttc aatttgttgc agggatgcag ggtgctgact atatagtagc aataaataaa
    48901 gatgataaag cgcctatact tgatgtagca catttagctt taataggtga tatttatgat
    48961 attattccta aattgattga aaaaatagaa aataacaaag ataataatca aaaatatatg
    49021 gcatcagcag ctaaataggg ggatagaaat gtataaatta atcgataaaa aagatataga
    49081 ttttttaata gatacttgtg gagaagaaaa tgtattagtt ggaagtgata taaatgaaga
    49141 tttttcacat gatgaacttg gagggattga gaagtaccct gaagttttag taaatgtact
    49201 tgaaactgaa caagtatcta aaataatgaa atacgcatat aaaaataata taccagtcac
    49261 accaagaggt caaggtactg gtcttgtagg tgctgctgtt gccataaatg gtggaataat
    49321 gattaatcta tgtaaaatga ataaaatact tgaagttgat tatgaaaatt taacattgac
    49381 agtagaacca ggtgttttat tgatgacaat aggtcaatat gttcaagata gagatttatt
    49441 ttacccacct gacccaggtg aaaaaagtgc tacaattgca ggtaatataa acactaatgc
    49501 aggtggtatg agagcagtaa aatatggcgt tacaagggat tatgtaagag gtcttgaagt
    49561 tgtactacca aatggagaaa ttataaatgt aggtggaaag gtggttaaaa atagttctgg
    49621 atatagtata aaggatttat tagtgggctc agaaggtact ttaggaatag ttactaaagc
    49681 tatattaaaa ttgcttcctt taccaaagaa aagtataagt ttgcttatac catttccaga
    49741 tttatcaatg gcaatagaaa ctgttcctaa aataataaaa tcaaaatcaa taccaacggc
    49801 tatagaattt atggaaagag atgtgatatt ggcagcagaa gaatttttag gtaaaaagtt
    49861 tccagataat acatcagatg cttatctttt gcttacattt gatggaaata gcactgaaga
    49921 tatagaaaaa gaatatgaaa aagtagctaa tttatgttta gaaaatggtg cattagatgt
    49981 atttatatca gatacacaag aaagaaacga ttctatatgg tcagccagag gtgcattttt
    50041 agaagctata aaggcatcta cgacacaaat ggatgaatgt gatgtcgttg ttccaagaga
    50101 taagatagca gagtttataa gatacactca cgagcttcaa gacaaattaa aaataagaat
    50161 aaaaagtttt ggtcatgcag gagatggaaa tctacacata tatattttaa aagatggtat
    50221 ggatgataat acatggaaaa taagattaaa agaaactttt gactatatgt ataaaaaatc
    50281 tagagaactt agtggtcaag tttctggtga acatggcata ggatatgcta aaaaagagta
    50341 cttacatgaa tcaaattcag atgcttatat gatgttaatt aaaaatataa agttagcttt
    50401 tgaccctaaa aatattttaa atccagggaa aatatattag aaatattaat ttaataatat
    50461 ttacaaaaca aaaaatatta aattagattt aaatataata ttttaaactt tacttttaaa
    50521 tctgaagcta aattgtaaaa tttttaggat ggcaatattt acttaaattt agaaagtaac
    50581 ataatattta agttgatgtt gtcatcctaa aagcatgtgt acataaataa agtaagacta
    50641 ttgtatctta tataataaag agttaataaa atgattttta taaatatagt tattttaaaa
    50701 aactacttta catatattgg atgagtataa aaatccatag ggccttttac taaatcttta
    50761 ttgtttttat aatatattga attatatata tttgtagctt ttatatcata acttatgagt
    50821 gttttaaaaa tcgtatcatc taatgaaaat gatatatttg aaaacttgtt tattattttt
    50881 atttcagatt tattttttat ctcttttaga atttctctac ctttatcatt aaaagcaagt
    50941 attcttatat aaggtatagt atctatgttt tttataagtt gcatgtcgct ttttttaata
    51001 cctaacaata tattgtttag agttcgtttg attttggtta aagtgtatcg cttggattta
    51061 attgctaatt gtagttcatg tagaaattca gatgtaaaga tatctttata gattctgttt
    51121 tctatgcctt catttacttc aaaatactct tttaaagtgt ccatatctct aagtataatt
    51181 gtttttaaaa tatcaaagaa gaagtcatca aacataggta aaaaaccatt atctattttt
    51241 tcatttagga tgttataagt tttttctgga ataacatttt ttaaatgaaa aaggtctgta
    51301 ttttctttaa gtgaagttct tatagcagta gcagaacaaa tatcactctc aatttcttcg
    51361 gaattatact ctgattggac cctggagata gtaaatggtt ttatactact gtttaaatgg
    51421 atgaggtttt ttatgtattc tatacccagt atgttgtttg atgaatttaa tgtttttgat
    51481 aattgttcct ttgaataatt ttgtataaaa ttattttttt tgtcttgatg tatataatca
    51541 aaaagtgcat tagctctagc tgttggaaat aacagaccat catctaaata tttttttaaa
    51601 agtaacttaa attcatttgg ttcatttgct aaaatttcag ctatattata aagaatttct
    51661 gtatttcctt cttcactacc aaaacatata gaatttatgc agtttagtga atttaaaatt
    51721 gttatagcac catgagaaaa aatttcagca ctttgacaag caaataaagt tggtaattct
    51781 ataactaagt ctacaccatt ttcgacagca atttttgctc tagtgtattt atcaaataaa
    51841 gctggttcgc ctctttgtag aaaatttcca ctcattattg ctacagtatg tgttgcattt
    51901 gttttttcta tagatttaga taaatgatat atgtgtccat tatgaaatgg gttatattca
    51961 actattaatc caagtatgtt cataaaatac tccttttatc tttaattatt ttatatatat
    52021 cacaatttat tataatatgt ataaaattta aatttttaat tataaaatta aaaaacgtaa
    52081 aaaaatttat aaaaatgtag aaatgttacc tacataaaaa actggttaaa tgttattaaa
    52141 ataactttat tagctcttta tattatatta ttataacaaa tgggaaagaa taatctacat
    52201 taagttaact tgttaatata ttattagtga aatacacaat ttaccatact agaaatatga
    52261 tataatataa aaaaaaagta tatactttta tattaaaagg tgatatatta tttatgaaaa
    52321 tcgtttaatg tatacaataa tatatctata acaaaataat aaaagataga gttttattta
    52381 gacatttatt acaaatcaaa atttattaca tattatttat aaaaaaatct aaggaggaaa
    52441 taataatgaa aatattagta ttaaactgtg ggagttcttc attaaaatat caattaatag
    52501 atatgaataa tgaagaagtt ttatgtatag gattggttga aagaatagga atagaaggtt
    52561 ctatattaaa acatgaaaaa gcaggtagag atgataaata cgttgttgaa caaccaatga
    52621 aggaccacaa agatgcaata gcattagttt tagaagctgt tgctcatcca gaatttggtg
    52681 cagttaaaga aatgaaagaa atagatgcag taggacatag agttgtacat gctggagaaa
    52741 aatttgcaac ttcagtagta ataactccag aagtagaaga agctttaaaa gaatgtatag
    52801 atttagctcc acttcataat ccagcaaaca taatgggaat agatgcttgt aaagctatac
    52861 ttccagatgt accaatggta ggtgtatttg atactgcttt ccatcaaaca atgcctaaat
    52921 cttcatactt atatggttta cctcatgagc tatatactaa atatggggta agaagatatg
    52981 gattccacgg aacttctcat aattatgtat ctcagagagc agcagagata ttaggaaaag
    53041 atataaaaga tttaaaaata gtaacttgtc acttaggaaa tggtgcttct atagctgctg
    53101 ttgatggtgg aaaatgtgta gatacatcta tgggattcac tccattagaa ggattaataa
    53161 tgggaactag atgtggagat atagacccag ctatattacc attcttaatg agaaaagaag
    53221 gtttagatgc tgatggattg gataaattaa tgaataaaga atctggagta tatggaatga
    53281 ctggaatttc tagtgacttc agagatatag aagatgcagc taaaaatgga gacgaaagag
    53341 ctcaagctac attagaagcg tatgttaaaa aagtacaaaa atatataggc gcttatgctg
    53401 ctgaaatgaa tggattggat gttgtagtat ttactgcagg cgttggtgaa aatggaaagg
    53461 ctataagagc agatattgct tctaacatgg agtttttagg aatgaaatta gataaagaag
    53521 ctaatgatgt tagaggaaaa gaaacagtaa tatctactgc tgattctaaa gtaaaaatgc
    53581 ttttaatacc aactaatgaa gaattaatga tagctagaga tacattaaga ttggtaaaat
    53641 aattaaatta aacctatgcc tatcaagtaa aaatacttga caaacaaatg ctagatttgt
    53701 ataataaatt tggtgatatg ttgaggtgaa cttaatgaaa gttagcatag aaaaaataaa
    53761 tagaaaagaa actgacaaaa tagacttgaa tttttgtgaa aaaattgata ctattagtta
    53821 ttgtgatgag atttacaaat tagtatcacc tgtaaattta aaagggaaag tgtcaaagac
    53881 taataaaggt ttgtatctag atatagatgt caattttaca attgttgaca attgttcaag
    53941 atgtcttaaa gaagtagaaa taccactaga gtattctata caaggttttt tagtaaaaga
    54001 agaagattat gatgaagatg agtttgagga atttgatcct tttatattcg atggtgaaga
    54061 aatcgacctt attgatataa tagaacagac attagatttt aatgtacctc ataaagttct
    54121 ttgtagtgaa aactgtaaag gtctttgtca agtatgcggg gcaaacttaa atgaagaaga
    54181 gtgttcttgc agtgaaatta caaacgatga ggaatacata gatcctcgtt ttgctaaatt
    54241 aaaagattta tttaattaaa ttttgactaa ggaggtggcg taaatggcag taccaaagcg
    54301 taaaacgtct aaatcaaaca caaaaatgag aagagcagct aactcaaaga tggaagcaac
    54361 aggatttgta agttgcccac aatgtcatga gccaaaatta ccacatagag tgtgtccaga
    54421 ctgtggatat tataaaggta aagaagttgt gtctaagtaa tagcttctaa aaagcatgta
    54481 gagtatactc tacatgtttt ttttgtacat ataaaaaaat aagatgattt actagagaaa
    54541 ataagtggat tttatgcata ttaaaaaata gaaaaataat aatataagta ttgaaaattt
    54601 ttgtgtgtta ctatatacta ctattagtag ctggtactaa ttacagctat aaaatttgga
    54661 gggaacatga aaaagaaaag taaagcccaa agacaaaaag agcttataga tatgctaaag
    54721 acagatcctt tttatactga tgaagaatta tcaagtctgt ttgatgtaag tatacagacc
    54781 ataagattag atagaatgag tctaaacata ccagagctaa gagaaagagt caaatcaata
    54841 gcagaaactc agagttcaaa agtaaagact ttaggagtaa aggaaattac aggagaaatc
    54901 attgatttat ctgtagggcg attaggtata tctatgttag aagttacaca ggacatgatt
    54961 tactctaaga ctaatacttt aaaagatacg tatatttttt ctctagcaga ttcattggca
    55021 atggctataa tagatgcacc taaggttatt atgagagttg caaatgtaaa aagttttaag
    55081 ttgatagaac aacaagatag acttatagca aaagcagaag tgtatagaaa tatagataaa
    55141 aagcattatg taaaagttgt tatcaacaat aaagcccaag agcaaatatt tagaggaaag
    55201 tttatctttg aagaattaga ttaggaggga aattatgaaa atagtaattg atggaatggg
    55261 tggagataat gcacctaaat ctaatgtaga aggcgcagta aatgctataa aagaatatca
    55321 ggttgattta ataatcacag gagataaaga tttacttgaa aaggaatttt caaattatga
    55381 atttgataga aataaattag agatagttca tactactgag attatagaaa acgaagataa
    55441 accagtaaag gctataagaa gtaaaaaaga ttcatctatg gtagtagcat taaatcttgt
    55501 aaaagaagga aaggcagatg ctataatatc agctggaaat acaggagcct tacttgctgg
    55561 tggattattt gttgttggta gaataaaagg aatcgataga ccttgcttat gttcagcaat
    55621 accaaatgta aagcgaggaa tgacacttat agcagactgt ggcgcaaatg cagattgtaa
    55681 acctaagaat ctagttgaat ttgcagctat gagtaatatt tattcaagaa aagttttagg
    55741 tcttgaaaat cctaaggtag cattagcaaa tgttggacta gaagaaggca aaggcaatga
    55801 tttagtcaaa aggtcatatg aagaaattaa aaaattagat ttaaatttta tagggaatgt
    55861 agaagctaga gaagttataa atgcatacac agatataatc atatgtgatg gatttacagg
    55921 taatatatta cttaaatctg ctgaaggtgt agcactttca gttatgagtc ttataaagga
    55981 aacttttatg gccagtacaa aaagtaaaat aggagcatta cttataaagg atgatttaag
    56041 aaaattaaaa agttttatag attattctga gtatggggga gctccacttt taggtctaaa
    56101 tggaggagtt ataaaagctc atggtagttc tgatgctaaa gctataaaaa atgctataaa
    56161 tcaaggaata aaattttcaa agggaaaagt cgttgaagat attaaccaat ttataagcaa
    56221 atacaatgaa gaaaataaaa ataacgaaga tgaataaact cgttttggag gtttaatata
    56281 taatgaatac aaaggctgga atattaggag taggaagtta tctacctgag caatcatatg
    56341 ataattttca cttcgaaaaa ataatggata catcagatga gtggataagt actagaactg
    56401 gtattaagga aagaagattt gccaaagagt ctgaagcaac ttctgactta gcatctaaag
    56461 ctgccttaaa agctatagaa tgtgcaaagt taaacgtaga ggatatagag ctgattatat
    56521 tagctacaat tactcctgat atgtctttgc cttcaactgc atgtatagta caagatgcaa
    56581 taggagcagt aaatgctact gcatttgata tatcagcagc ttgttctgga tttgtatatg
    56641 gagtaactat tgcaaagcaa tttgtggaaa caggttgcta taaaaatgtg cttgtaatag
    56701 gagcagaaac ttgttctaag tttttaaatt atgatgatag aacaacagca gtattgtttg
    56761 gagatggagc tggagcagct gttattggcc ctgtaaatga gggtggaata ctgtctacac
    56821 atatgggctc tgatggtaaa ggaaaagatt gtttaaaagt acctgctgga ggttcaagac
    56881 ttaaggcaag taaagagact gtagaagcta atctacatac aatagaaatg gcaggtagtg
    56941 atgtgtttaa atttgcagtt agaaagatgg cagaaacttc tttaagagct cttgaaaaag
    57001 caaatctaaa tacaactgat atagactact tagtgccaca tcaagctaat ataagaatca
    57061 tacaagcttc ttctaagaga ttagagcttg atatgaaaaa agtttatgta aatatagaca
    57121 aatatggaaa tatgtctgct gcatctatac cagtagcatt agatgaagct tatagagaag
    57181 gtaaaattaa aaagggtgat aatgtagttt tagttggctt tggtggaggt ctaacttggg
    57241 gggcttcagt tgtcaagtgg actttatagt ataaaatatc taaaatgatt taattaacaa
    57301 aattaaactt atacacaaaa taaagttgat taataataaa aagtttactt ttatgatttc
    57361 aagtgaagga gaattgtatg aataaaattt gcaaaatatt aaatataaaa tacccagtta
    57421 tccaaggggg aatggcatgg gtagctactg catcattagc aagtgctgta tctaatgcag
    57481 gaggacttgg cataatagca gcaggaaacg caccaaaaga agctataaag aaagaaattg
    57541 ttgagtgtaa aaaattaaca gataaacctt ttggagtaaa tgtaatgctt atgtcgccat
    57601 ttgttgatga tataattgat ttgattatag aagaaaaagt tcaagttatt actactggtg
    57661 ctggaaatcc tgcaaagtat atggatagat taaaggaagc tggaacaaag gttattcctg
    57721 tagtacctac aatagctttg gcacaaagaa tggaaaagct aggagctaca gcagtaatag
    57781 cagaaggtac tgaaggtgga ggacatatag gagaacttac tactatggtc ttagttccac
    57841 aagttgctga tgctgtaaac atacctgtaa tagctgctgg aggaattgta gatggtagag
    57901 gaattgcagc atcatttgca ttaggtgcca gtgcagttca agtaggaact agatttattt
    57961 gcagtgaaga gtgttctgtc cattcaaact ataaaaactt agtactaaaa gcaaaagata
    58021 gagatgcaat tgtaacagga agaagtactg gtcatccagt aagaacatta aaaaataaac
    58081 tatcaaaaga atttttaaag atggaacaaa atggagctac tcctgaagaa ctggataaaa
    58141 aaggtacagg agctttaaga tttgcaacag tagatggaga catagaaaaa ggttcattta
    58201 tggcaggtca aagtgctgct atggtaaaag aaataacacc ttgtaaggaa attatagagg
    58261 ctatggtaaa tcaagcaaga gagattatgc cagcaataga actgtaaatc aggaggagca
    58321 agatgggaaa agttgcacta gtattccctg gtcaaggagc tcaatatgta ggtatggcca
    58381 aggatttata tgaaaataat actgttgcta aaagtgtaat agatgaggct agtgatgcct
    58441 taaatatgga tttaaaaaac cttatgttta atggaaatga agaagaactt tctaaaactg
    58501 aaaacactca acctgctatg gttacacata gtgtagctgt attaaaagca gtacaagccc
    58561 aagtagattt aaaatatagt gcatgtttag gtttatcttt aggagagtat agtgcattgg
    58621 ttgcagcaga tgcaattgac tttaaagatg ctgtatgctt ggtcaaaaaa agaggtaagt
    58681 ttatgcagga tacagttcca acaggtgttg gagctatggc agcaatttta ggtctagata
    58741 gaagtatatt agaaaatgta gttaaagata tacaaggagg tattgtggaa gtagcaaatt
    58801 ataattctcc tggtcaaata gttatatctg gtgaaaatga aaagatagaa gaagcaatgg
    58861 tgaaatgcaa agaagcagga gcaaaaagag ctgttaaatt aaatgtaagt ggaccttttc
    58921 actcatcaat gctaagagaa gctggtgtaa aattggccag tgaattagaa aaagttaaca
    58981 taactaaacc taaagttgat gtggtggcaa atgtaaatgc agattactat aaaaacgaag
    59041 gtaaagacct gcttataaaa caagtaagtt catctgttct ttgggaagat tctatagaaa
    59101 gacttttaaa tgatggatat gatactttta tagaaatggg acctggaaaa actttaaaag
    59161 catttattaa gaaaatagct tccaataaaa aagcaaatgt caatatttat aatatagatg
    59221 gtatagattc tcttaatgaa tttgtacaaa attatagaaa tggagagata taatgataaa
    59281 tcttacagga caagtagcag ttgttactgg tggctctagg ggtataggaa aagaaatagc
    59341 aaaaaaacta gcatcttttg gagctgatgt agtaatcaat tatacttcta aagaagatga
    59401 agcactaaaa actaaaaatg aaatagaaag tatgggggta aagtgtacct ctataaaatg
    59461 tgatgtgtct aaatttgatg aagtgaatca aatgatagat tctgttgtaa gcgaatttgg
    59521 aaaaattgat atattggtta ataatgcagg cataactaaa gatggtctgc ttatgagaat
    59581 gaaagaagaa gattttgata gagttataga tataaactta aaaggtgtct ttaattgtac
    59641 aaaagcagtt actaaaccta tgatgaaaaa gaagtatgga agaataataa atatgacttc
    59701 agtagttgga attatgggta atgcagggca aactaattat tgtgcatcaa aagcaggtgt
    59761 aattggattt acaaaagctt ctgcaagaga gttagcatca agaaacataa atataaatgc
    59821 agtagcacct ggatttatag aaacagatat gactaaagta ctaagtgatg atgtaaaaga
    59881 atcaacacta gcaaacatac caaagaaatc ttatggtaaa ccagaagatg tagccaatgc
    59941 cgtagcattt ttagttagtg acatgtcaag ttatataaca ggacaagtaa taaatgtaga
    60001 tggtggaatg gtaatgcaat aataatttaa aaagtagaga aatctattaa aataaaaaat
    60061 tttaggaggt acacaatgtt taataaagtt gtagaaataa taatagagca attaggagta
    60121 gaagataaag aaataactat ggaaacatca ttaatgaagg atttagaagc agattcatta
    60181 gatgcagtag aaataataat ggcacttgaa gatgaatttg gtatagaaat accagataca
    60241 gaagcagaaa acttcaaatc tataggagat atagttaagt atatagaagc taataaatag
    60301 tcgagcttta aaaaagtgga ggtatccgat gaataaaaga gtagtaataa caggacttgg
    60361 atgtgttacg ccgttgggaa caggaaaaga agagttctgg agtaatataa aatctggagt
    60421 atctggtata gataaaataa ctaattttga tgctagcaca tatcaaactc agatagcagg
    60481 agaagttaag aattttcatc cagaagaata tataagtaaa aaagaattga agagattaga
    60541 taaatttgct caatttgcaa tagtatctgc taaattagca gttgaagatg caaatcttga
    60601 tttagataaa gttgatagag aacgttttgg tgttataata ggttctggaa taggtggagt
    60661 tgaagctata gaaactcaac ataaaattct tttggaaaaa ggaaataaaa gagtaagttc
    60721 actttttgta cctatgatga taggaaatat ggcagctggt caagtttcaa tatttttagg
    60781 agcaaaaggg cctaacacta atgtatgtac agcttgtgca tcaggaactc attcaatagg
    60841 tgatgctttt aaggtaattc aaagaggtga tgcagacatc atggtagcag ggggttctga
    60901 agctgctgta acaggtcttg cttttgctgg attctgtaac atgaaggcta tgtcaactag
    60961 aaatgatgac cctaaaactg catcaagacc atttgataaa gacagagatg gttttgtaat
    61021 gggagaagga gcaggtattg ttatcttaga agacttagaa catgctttag caagaggcgc
    61081 taaaatatat gcagaagtag ttggatatgg tttaactgct gatgcatacc atatgacaac
    61141 tccagctgaa aatggagagg gagcagctag gtctatgaat atggctttga aagatggaaa
    61201 tgttccatta gaagaagtag attatataaa tgctcatgga acttcaactt attacaatga
    61261 tttatatgaa actatggcta taaaaactgt atttggagaa aaggcatatg acttatgtgt
    61321 gtcatcaaca aaatctatga ctggtcactt attaggagca tcaggtgcta tagaggctgt
    61381 tgtttgtgct atgagtatag aagatagttt tgtgcctcca actataaaca tacaagaagt
    61441 tggtgaagat ttggatttag attatgttcc aaatcaaggt aaagaaaaaa atattagata
    61501 tgcactatca aattctttag gatttggtgg gcataatgct acaatagtac taaaaaaata
    61561 tgtataaata aatattaaat ataaaactaa aaaacaagca cttgttttaa ctgtataaat
    61621 ttatattagc ttaaatttaa agtctcctaa attattttag gagacttttt tattaagatt
    61681 taagtctagc atatgttgta aatcattttg atatattgta ttattaatta ttaagaggtt
    61741 atttatatat gtaagttttc attctacaat atacaaaagg aggactaaaa ttttgaattt
    61801 tagactacca aagaaaaggt tacccaaaaa gataattttt atatgtataa tacttattag
    61861 tattattttg tttataaagc atgaccaaaa tggattgaaa gaaagagaaa ttataaataa
    61921 gggaaaatat atgtctgaaa gccttgtggc agataaggag tatgatgaag caaaaaaagt
    61981 ggttaagact tctttcgaat ctattccaga aaaaaaatac aataaagtat ataatgaagt
    62041 ttgggatatg ttaaagacta tttcatatgt tccagatgga acgaagatag tcattgattc
    62101 tttagaaaag atgagagaat cagatgtgaa attatctgat gaatgtagat ttaatataga
    62161 aaaaagatta gcatcagtgt atataatgga taataattat tttaaggcag tagatttaac
    62221 tgtaaagtct ataaagttgg ctgagaaatt ggggaataat tatgaaaaag caagactaga
    62281 tgttgatttg gcgagcatat ttttaaagat aggtggctat gaaacaggag agaaattaat
    62341 aaaggattct tttaaaatag atattgatga aggtgaaaaa aatggaatgg ttagattata
    62401 tgctattata aatctagcag aaatatatgt agaagagggc gaatatgata aagctattga
    62461 aataagtagc aggataaaag attataaaaa gtttgtaaat acggataatt acaatgattt
    62521 tgatataatg gcctctataa tgcagtcaaa tgcgtatgtg ggcaaaaata atatacaaaa
    62581 ggcaaagact tttttagaaa aagctgatta tcttataaaa tcagatagaa cagtttatat
    62641 attagataaa gatatgtact attatatggc aatgggaaac ttagaatata aaagcgaaaa
    62701 ttataattct gcaatagata attataaaaa aagtttagag atatctacta aaagaaagtt
    62761 aacccaggag aaaataaaaa atttaaataa gatattaaat atatatgaaa aacaaggaaa
    62821 tatagaatct ttaaatgagt atcaaaagat gttaataaat gaatataaag agaacaaaag
    62881 tataagagat tcagagacta gtttctatat aattgataaa gtatcaaacg aaagtaagct
    62941 atttgaaaaa actaaaaaag aaataaaata ttataaaata ttatttacag taattttaat
    63001 tgctatatta gggtctactt ttttatacaa ccgattgaag tattttaaag cacaaaattt
    63061 acatgatggt cttacaaatg tttataatag aaaaagtttt gatattatgt atgaaaagta
    63121 cagagagaaa gatgataatt ttgcacttgt gatgatagat attgataatt tcaagctcat
    63181 aaatgataat tatggacata aatttggtga cactgttata aagggaataa caaataccat
    63241 ttgtgttatg ttagaacaag atgataaagt ctttaggtac ggaggagaag aattttctgt
    63301 attaataaga aataaatctg gtgatgaagt agcagatata attgacagta taagggttgc
    63361 aattgcaaat aaaaagtgga atgatgatgt gactgtgact ataagtgcag gtgtagctca
    63421 tgtttcagat agcaaagatg tactagaaga agctgataaa aatttatata aagcaaaaca
    63481 acttggtaga aataaagtag tatataagta attatatact atataaataa gaggtataaa
    63541 tatgaaacaa tcaatagcta tagtaactga taaaataact aaactatcat ctttcttaga
    63601 agaaaatata cgtttggttt taggtaatta tattgatata aaccactatt atctggaagt
    63661 tttaaaagag aatgataaaa tacaaggtga ttttgtacta gtaatgaatg atgatagatt
    63721 aaacagtatg agaaagcatt tagtacaaga tagtaagatt atagtgataa ggagaacact
    63781 taaagaaaat gaaatatatg gattattttc aataccaaat ggaactgaag tattggttgt
    63841 aaatgataca aaagaaacta ccttagaaac gataagctta ttttacaaaa taggagtaaa
    63901 aaatttaaag tttactccat atattgaagg aaaaatttat aaaaatatag acatagctat
    63961 aacgcctgga gtaaagggaa aagtaccaag ttatataaat aaagtaatcg accttggaaa
    64021 tagatatatt gatatatcta cctttataga aataataaat ggtcttagaa tagatgttaa
    64081 agaaatacgt aaaaatttaa tggcatattc tgagagttta ataagtttag ataatggagt
    64141 taaagataat tataggcaac tgtttttaaa aatagaagaa caagatgtta tattgaatct
    64201 atcaaaagat ggaataatat ttacttcagc agatggtata ataaacacat ttaataaaga
    64261 agctttaaaa attttaggta taaatgaaaa tccacaaggc aaaaacatag aagatatcat
    64321 ttctgaagat ttaaatattt tacttgaaga caaagaaata attgatgaag tggtatttac
    64381 aaataaaaaa tatataaatg taaataaaaa aagtattttt tctatgggga ataaagctgg
    64441 gatatattat aacttacaag aaataacgta tataaaaaaa ttagaacaaa atcttactaa
    64501 taaattaaga gaacaaggtc agatagcaag atatacattt aaagatataa aaactaaaaa
    64561 taaaaatatg gtaaagtgtg tagaattggc taaaaaaata tctaaatcag aactttctgt
    64621 attaataata ggagaaagtg gtactggtaa agaattatta tcacaatcaa ttcataatgc
    64681 ttccaataga agtaatcaac cgtttatagc agttaattgt gcagcagttc cagatagtct
    64741 attagaaagt caactgtttg gatatgaaaa aggctctttt acaggagctt taagagaagg
    64801 aaaaaagggt ctatttgaac ttgctaataa tggaactata tttctggatg aaattggaga
    64861 tatgcctcca ttgctacaga caaagttact aagggtatta caagaaaagc aggttatgcc
    64921 tataggttcc cataatgtta taaacataga tgttagggtc atagcagcaa caaacaaaaa
    64981 cttactaaag atgattagag aaggaaaatt tagagaggac ctgtattata gactaaatgt
    65041 attgccaata gatgtaccat cattgaggaa tagaaaagaa gatataataa cacttatgaa
    65101 tttctttttg aaaaacaata taagactttc gccagaagtt ataactattt tagaaaaata
    65161 taattggcca ggaaatataa gagagttaca aaatgttgct tcttatgtaa gtctaatgtg
    65221 tgatagtatg gtttttagag atgacttacc accatatata agggtagaat gtgctaacaa
    65281 ttatgaagat ggatttggtt atacaagaaa agaatttgag gaaatactga ggattctata
    65341 tgaaagaaga gagtcatcct ctggaattgg aagagggttt ataatcgaaa agcttataca
    65401 ggaaggtatt gagatttctg aaagcaaagt tagaaaggtg ctatcttatt taagtgaatg
    65461 tggatacata tcatctaaat ctggtagagg aggaagtcaa ataacctcag aggggataaa
    65521 actttataat aatataaatt aagctaagta tttgatattg aaatcacata ttttgaattt
    65581 agatatttaa ataataataa acttttatat ttatagagat taatttatat tatgtcagaa
    65641 agaatattgt aatttataat taaatattaa taggttttta aaataggatt agataggtaa
    65701 taggttaagt tagaataacc cattacctat ttttatttgt aagaaaagtg tacatagaat
    65761 tagaagtaaa aaatacaact aataaaacta ttgaaaatac tagataaata ataaataaaa
    65821 ataattatag attttggcat acatattgct aattatagag ataagacgac aaaataattg
    65881 ggaggtttaa tgatgaaata caaaacaaca gggacttgtg ctacagaaat tgaatttgaa
    65941 gtaaaagaaa ataaagttac aaatgtaaac tttataggtg gatgtgatgg aaatttaaaa
    66001 gggctaaaag tattagttga aggcatgaat atagaagatg taatacaaaa gttaaaagga
    66061 atagagtgta aaacaaagcc aacatcttgc ccagaccaac tgtctctagc attggaaaat
    66121 tatatgaata taaagtaata cataaaacaa tcgattagtt aggagtttaa ttatgaaacc
    66181 aataataggg attttaggaa atttaataat aatggaaaat ggtatgtttc caggtcttga
    66241 aaggtcatat gtaaataatg attatataaa tgcagtgcta aaaggaggag gtagtccagt
    66301 aataattcct gtaaatacag ataaagaagt tataaaaaaa cagattgaaa tggttgatgg
    66361 agtcttaata tctgggggat gggatatcaa tcctcaactg tatggagaag aaacaagaga
    66421 agaaacgaca tttatatatc cagaagtgga tgaatttgat ttaattgcaa taagtatagc
    66481 attagaactt aaaaagccta tattaggtgt atgtagggga ctacaaattt taaatgtaag
    66541 tttaggagga actttatatc aagataataa tcttatagaa ggaagttata taaaacatac
    66601 acaatcttct aaaagacatg tagctactca taaagttgat gttaaagaag gaagtatttt
    66661 agagggaatt ttaggaaaac aattacttac aaatagttat catcatcaaa gtgtcaatca
    66721 gcttggaaaa ggattaaaag ccatagcata ctcaaaagat ggtataatag aagctattga
    66781 gaaagaggat gaaaattttg tagtaggagt acagtggcat ccagaaatga tggtagatta
    66841 ctgtgataaa atggaaaaac tatttaaata ttttataaat atttgttcaa atttataata
    66901 taccttgggg ggattgaaca tgaacgaaaa gaacaaaatg ggattaataa gtattatact
    66961 tttaggtatt aatgcagtag taggagctgg agtttttcta ttaccaggag atgcaatgaa
    67021 atcatttggt gtagcaagta tatttgtata tatatttgat atgttgttag ttttatctat
    67081 ggcattctgt tttgctgagg ttgcaggtaa attcaataaa aatggtgctg cctatgtata
    67141 tacaaaagaa gcttttggag atttttgtgg ttttgaagta ggtcttatga aatgggtaat
    67201 aggatgtatt tcttggggag ctttaatagt aggttttcct acatcattat cagcagtatg
    67261 ggctccagca ggtgaaccac atatacaaaa aattataatt gtagtcatga tagttgggct
    67321 tacaattata aacttattag gtgtatctct atcaaaaatt gtacaaaatg ttataactgt
    67381 aggtaaacta attccattaa tactatttat aggaataggt atatttttta taaaaggagt
    67441 taattttact tcttcaacaa tggtacctcc aggagctgga gcaacagagt ttggagcagc
    67501 agcactttta atgttttatt catttacagg atttgaatct atagcagttg cagcagaaga
    67561 tatggagaat ccacaaaaaa atataccaat agcaattata agtgtaatag taattgcatc
    67621 aataatatat atattaaatc aagttgtatg tgttggtata ctaggagatt cgttatctag
    67681 tacatctaca ccagtggcag atgctgcaag aatatgcttt ggaaatatgg gagctggatt
    67741 ggttaccttt ggaaccttgg tttctgttgg tggaatttgt atgtgtggtg cttttgtaaa
    67801 tccaagaagc tgtgtagctt tagctgatga taaaatgtta ccgagaatat ttgcaagaaa
    67861 agataaaaaa ggtacaccat atgtagcgat aatagctact atgctaatca ctataccaat
    67921 agctctatct ggaagttttg ctgaattagc agcaataagt gcagttgcta gattcataca
    67981 atacatacca acatctttat ccgtattagt gtttagaaag aaaagaccag aattagtagg
    68041 tacttttaaa actccttttg gagcagtgat tcctctaatt gctgtatgtg ttggaacatg
    68101 gttattattc caagcatcta tgcatcaatt aattatggga ttaggagctt tggcaattgg
    68161 agtaccatta tactttataa tgaagtctta taatagaaaa gtctatgatg aaaatttaaa
    68221 gaaaatagtt taattatgtt ataacaattt attgagttaa aattagacat tggcataaaa
    68281 ttaaatacaa ataagtatct acaaggctat attaaaatat tattaatttt ttattttaat
    68341 atagccttat attgttatca ataaatgaaa aatattgtaa caatagaaga acaatagtat
    68401 tatgtttata gacaaaaaac ttattaggag gtaaatttat gccaacaata acattaaaaa
    68461 atggtgtaga cgtacttatt agagaaggtg taagagaaga tgctcaaagc attatagatt
    68521 tttataatga agtgggtgga gaaactcatt ttctgtcatt tggaaaggat gaatataaaa
    68581 tatctttgga ggaacaagaa aatgctatag aatcagcaaa agcatccgat aattctgtta
    68641 aactgattgc atttatagat ggtgaaatag ttgggattgc aacgattgat tctaatcaaa
    68701 aagcaaaagg aaagcatgtt ggagtattag gaattgttgt aaaagaaaaa tattggggaa
    68761 ttggattggg taaaaggctt atgttagacc ttatagaatg gtgtaaatca aatggaataa
    68821 ccaagaagat aactcttgtc actaatgaag aaaattataa tgctatagga ttatacaaaa
    68881 aagttggatt tgaagtagaa agcatactaa agaaagaatg ttattataat ggtgtttata
    68941 ctgatttaat aggaatgtca cttttattgg gaatataatt agtgtacatt ttatctcgaa
    69001 aaaagtatat taagtatggt ataattaaaa aagaatataa cttaatataa ttttggagga
    69061 cgaattatga aaaatctgtg taaaatatca gatgattatt taaacagcaa gcttaagtat
    69121 ttaaagttat tatcaaaaca atatccaagt atatcaaaag caagtacaga aataataaat
    69181 ttagaggcta tattaaatct tccaaaggga acagagcatt ttataacaga tgtacatggt
    69241 gaatatgaac catttgtaca tgtattaaaa aatggttctg gtgtaataaa gagaaaaata
    69301 gaagaattgt tttcaaatac aataagagac agcgaaaaaa aaatgttagc aactcttgta
    69361 tattatcctg aacaaaagtt agacctaata attaagcaag aggaaaatat agatgatttt
    69421 tatagaataa atatttatag acttatagaa ctttgtaaat atgcttctag caaatatact
    69481 aggtctaaag ttagaaaact gttaccagaa aattttaaat acataataga agaacttttg
    69541 catgagcatg ttaaaagtga acataaagaa gagtattata aaagtatagt tgaaacaata
    69601 gttgacatag gaatagctaa ggagtttata attgctattt ctacagttat acaaaaattg
    69661 gttgtagata gacttcatgt aataggagat atttatgata gaggtccaag accagatata
    69721 attgtcgata agcttattga acatcattgt gtagatattc aatggggaaa tcatgatata
    69781 ttgtggatgg gtgcagcctc aggtgaaaaa acttgtatag caaatgcact gaggatatca
    69841 gctagatatg cgaatttaga cattgtagaa gacatatatg gaataaatct attgccttta
    69901 gccacttttg ctatagaaat gtataaagat gacccttgta aagaatttat tccaaaggtt
    69961 aatgaccaaa gtgtaacaac aacagaaaag tctttaatgg caaaaatgca caaagcaata
    70021 agtataattc aatttaagct tgaaggtgag gttatcagac gaagacctga atttgaaatg
    70081 gaacatagac tcttgcttaa tatgataaac tatgatgaag gtacgattaa tttaaaaggt
    70141 aaaacatata aattaaaaga tacttattta cctacaatag acaaaaaaga cccatataaa
    70201 ttaactatgg aagaaagaaa tgttattgat aaattagtat cttcatttag aggtagtgaa
    70261 aaattacaaa aacatgtttc atttttgttt tctaaaggaa gtatatattt aaaggctaac
    70321 tcaaatttac tgattcatgg atgtgtaccg ttaaatgaag atggaagctt tatgtctatg
    70381 aatataatgg gaaaagagta taaaggtaag gctcttatgg ataagatgga gtctctagca
    70441 agagaaggat ttttctttaa agataaggca gaagaaaaac tgtatggcat ggatattatg
    70501 tggtatttat ggactgggaa atgttcatca ttatttggta aagatgatat gactacattt
    70561 gaaagatact ttatagctga aaaagagact cataaagaaa ataagaaccc atattttaaa
    70621 cttagagaaa atgagatggc ttgtaaaaga ttatttgaag aatttgattt agaacttgat
    70681 gaatctcata taataaatgg tcatgttcct gtggagagta aaaatggaga gagcccaata
    70741 aaagctaatg gtaaaattct cgttatagat ggtggttttt ctagggctta ccaaaaaact
    70801 actggtatag ctggatacac tttgatatac aattctagaa ctttgcaatt agtatcgcat
    70861 gaaccattta attctgctga agaagccatt gcaaatgaaa gtgatatctt atcgacaact
    70921 gttgtagtag agcataaggc aaagagaaaa atggtgagag atactgatga aggagtaaag
    70981 atacaagagg aaatagaaga tttaaaacta cttttaatgg cttataagaa agggctaata
    71041 aaagaaatgt agatgttata gaggcacttc atgattgaag tgtctttttg ttaatactta
    71101 ttaaatatat tataaagatt aataatagta gtttatatta tacttactaa gtgaatccaa
    71161 gataaatggg ttcacttttt ttatttataa acattattaa aaaaatatag atgttttggt
    71221 tatattcctt gtcctttata aatagaatta tataaacaaa ggaggaggac aagagatgaa
    71281 taaactttct gatgaaataa taaactctct ttctacaact ataagagaaa aggtagaaaa
    71341 agtctcaaac aataatctga acatagaaga aattaggtta cgctcacaaa aaccattgat
    71401 actaaatgca aactctaaag attattttta caatcaaaaa actatgacac ttgatttaaa
    71461 tcaacaaaac tcatatgtag taacaagaga agatgtagag caaacttttc aaataatatg
    71521 taaatattca atacattcat ttatggatga cataaaaaaa ggattcataa ctttgagagg
    71581 aggtcataga gtagggttag ttggaaaagc aatagtagaa gatggacaag tcaaaaatat
    71641 aaagcatata tcatctttaa atataagggt atcaagggaa attattggat gctcggataa
    71701 gattttaagt catatcataa agggaaaaaa tcagattaat aatactttga taatatcacc
    71761 acctcaatgt ggaaaaacaa ctttaataag agatatagta agaaatttaa gtaatggtaa
    71821 tgaagattat ggatttaaag gtttaaaagt ggctttagta gatgagcgta atgagatagc
    71881 aggagcttat ctaggtgttc cacaaatgga tgtgggaata aggactgata taatagaaac
    71941 ttgcccaaaa gacttgggta ttacaatgct tttaaggtct atgtcaccaa atgttattgt
    72001 gacagatgaa attggaagtg aaaaagaaat aaaggcatta tacacagcat taaatggagg
    72061 tataggatta ataactactg tgcatggaga ttcaatagag gatatacaaa atagaaaaga
    72121 attaaataga cttctggata aagagttatt taaaaaggtt ataattcttt ctgcaaaaag
    72181 aggagcagga acaattgaaa aaatttatga tttagaagaa aagagatggt attttgcaaa
    72241 ttaaaataat tattatagct ttcttgatag gaagtagtta tttgataggt gaacaaatat
    72301 acaaaactta tactcgtaga cataaacaat taaacgattt aataagagtt ttagaaattt
    72361 tgaggatgga tttatctttt ggactttata cattagaaga aatctttaat aggataggtg
    72421 gaaataaaga gttttgtttt tggaaatttt tctatcaaat atcagaagga cttcacaatg
    72481 aacaaagtaa aactttggaa ataataatat cagaaaatat tgatgtctta agtaaagaaa
    72541 cttatctagg caataaagaa attgaggaac tgaaaaatct aatacttact ttagggaaaa
    72601 gcgatataga gtcacaacaa agaatgatag atttatctat agaaaatttg aaaaaacaaa
    72661 cctatgagac aaaggaagat ataaacaaaa aaggcgtatt gtataaaaaa ttagtgacat
    72721 tcataggtat aggtatatgt attatattga tttaatatgg ggaggtttat tatggaaata
    72781 tcattgatat taaaggttgc aggtgtcggt attctaatat cagtattgaa tatgatattg
    72841 gaaaaaacag atagaaaaga ttgggcaggt cttactacat tagcaggtgt aataatcgta
    72901 ttgggtatgg taataacaga aataagtgac ttgtttaata ctgtaagaac aatgtttcaa
    72961 ctttactaaa gaggagggag taatttggaa ataatgcaat taataggaat tgcaatcatt
    73021 tcaactacac tctgtttagt aattaaaaaa gatagaccag agatagcaaa tttcatagct
    73081 ataataactg gagtaataat actattgtca gttatgttta agctaaattt tatagtagat
    73141 agtatacaag atttggctaa caaggctaac atcccgacta tgtatatatc attaataata
    73201 aaattaattg ggatagcata cttaatggaa tttgcgatac aactttgtaa agattgtggg
    73261 gaaggaaaca ttgcttcaaa actagaattt ggaggtaaaa taatagttat gtcaatgtct
    73321 ttccctatac ttttgtccat tgtagaaatg gtggtgaata taattccata gatgtgtatc
    73381 ttggaatgaa tataagtata ttagagagct tttgttgtat tgaggtttat agagtgaaga
    73441 gaggcatggt tgcttatgaa aaaaagattt ttaccaatga ttataggttt tttatttact
    73501 ttcttttttg taaatatctt tgctattatg atatttgcta atgaagtacc aagtagtgaa
    73561 gataaagaat atggtgaaac aaaaaatagc atagataagt atatagatgg tcaattagac
    73621 aagctagata taaatgagat tcaagactat ataaataaag aaatagttat taatgatgtt
    73681 aatttaaaat catttgtaaa agatttaatt agtggagaaa aaaatatctt agatttattc
    73741 aataaagatg gattgaagat attgatgttt gatgaattta aagcaagttt aaaggtagta
    73801 gcagtaattt tggtactggc tctgttatca tctattttaa agagtctgga aaactctttt
    73861 tcatcaggtg cagtaagtca aatagcaaca tatataatat ttatcacaat ggtatcactt
    73921 acacttattg gttttaaaga tgtattacag atttgctatg atgcaataga ccatactgta
    73981 ggtcttatgc aagttataat gccaatatta ataacatttt tacttttaat tggattccca
    74041 ataacgtcga ctactttgaa tcctatcttc ataggaggag taacatttat aaatgtattt
    74101 tttaaaaatt ttctatttgt atctattact gttgcatttg gaattttaat aataaacaat
    74161 ttatcaaaaa atataaggct aaagagattt ttctcctttg taaaacaaat aaattatgtt
    74221 tctattggag ctatgtttac tgtttactta ggtcttgtat ccatacaagg attgtatgta
    74281 accagttttg ataaattcag tgtaaagact gctaaatttg ctataggtaa ttttattcct
    74341 gttgtaggtg gatttgtatc tgactctgtg gatattttat tatcttcatc tcagcttata
    74401 aaaaatatat ttggaggaat agggcttatc ttacttgttg gaatttgctt acttccagta
    74461 ataaaaattt tatcagtaat agtagtgtac aaattagcag caataattgt agagcctgtt
    74521 ggagaggatg gaatatctaa ttttttaaat gaagtggcaa atttaatgat aataatgcta
    74581 gcatcagtaa ttgctataac agtaatgttc tttgtaactg tagctatctt gacctcgata
    74641 agtgtcgtaa gtcaagggta atttatatat cagtgaaaaa taattacatt taatctaatg
    74701 tttttagaga ggaggatgct atgttagagg gcataaaggc atggatagtt agtgttttga
    74761 taggagcatt tatagtaaat atagtagata tgattttacc aagttctaag ataaaacctt
    74821 atgtcaacct agttttaaac tttatgtttg tatttattgt aataactcca gtagtaggat
    74881 ttttttcaaa agatatgagt ttagaagata gaatattaaa atctatggga aactacaaca
    74941 agcagtatgt agatagtaca aatgctttgg caaaagagac tggaaataat agtctatcca
    75001 agggatatga ggatgggtta aaagaagtac ttaaattaaa acttgatgaa tatggttatg
    75061 atttagaaga tatagagctt aatggggcaa atataaataa tataaaaata aaagaaaaga
    75121 ataatagtgc aaaaagtaat aacagttcca atgaagaaaa taaaaataat agtactaaaa
    75181 gagaggaaga aaacataaac tctaatgaca aagaaaattc taaacaagtc ttcaaaaaag
    75241 gtacagagta tgggttgaac ctaaatgaag aaaaattaaa aaacgactta ataaaggtgc
    75301 tggatgtttc tatagaagat atacaaattg ataaatagga gttgggagaa tgttgaaaaa
    75361 cttaaatgac aaagataaga gaaaggtata ttcactaata actatagcag gaatatgtgt
    75421 ggtttcatta gtattattat cttgttttcc tagtagcaaa agcgataaac aagttggaaa
    75481 aacagagact aataaaaatg ctgaaaagca agtcactaaa gaacaagaaa aagatgattt
    75541 agagtcaaaa ttaacagcaa tactatctaa aattgatggt gcaggagacg ttgatgtaat
    75601 ggttacattt gaatcaagtg aagaaataca acctgctttt aattcaaata atacaacaga
    75661 aacaacagag gaaaaagatg cacaaggtgg agaaagaaca gttacgactt ctagtgaaaa
    75721 taaaacaatg ataacttcaa attcaagtga ccctgtagtt ataaaaacta cagagccaaa
    75781 gataaaggga gtgatagtgg tagcaagtgg agcaagtgac ccaagtgtta aagagacact
    75841 ttatagtgca gtacaaacat cattacaagt agctggtcat caagtagaaa tatattctaa
    75901 ataaatacat ataaccgggg ggataaatga atgaagttta attataaggg aagaggattt
    75961 gtaataataa ctttaactgc aatgttagtg gtagtaggaa cagtgaatta tcaactaagt
    76021 aagaaatctt tattagaaac atcaaaagag tttaaggcat atgaagaagc acagttgcaa
    76081 aaaaatactg atgatagtta tagtagtaag acagaggatt ctagtaatgt agataaacaa
    76141 ggtggtaagg aaagtgctga tattgatata gtcgatagca aagctagtaa agtaaaagaa
    76201 aaggcaactg agacaagtaa ggaaataaaa gctcaattat catcagagaa aaatatgaaa
    76261 aaggcatctt atattttaga tatgaaaatg aatagagaaa aacaaagaaa tgagttagtt
    76321 caagatttaa atgaaatgat aaataatcca tctacaacag aagaatctag aaaagaagct
    76381 tctaatatga aattaaatat agttaaaatt caagaaaaag aacttcaaat agaaaacctt
    76441 ttgagtacaa aaggatatga agaagcgtta gtttatataa gcgataacaa agttaatgta
    76501 gttgtaaatg aagctaaatt agaaaagaaa gatgcagcaa aaatatttga tttagttgca
    76561 gaacaagcca atgtgaagta tgaaaatatt aaacttacaa ataataatag taataagtaa
    76621 agtataaacc tcagaattaa ctgaggttat tttcttgcaa agtagacttg tatttggtat
    76681 tattatactt ataaaaatag ttccttaaaa tcaggaggta agttttaata tggaagataa
    76741 taaatttgga caagtaaaaa tatctaatga tgtaatagct acaatagctg gtttagcagc
    76801 attagaagtt gaaggcatag aaactacagc aacattaaca gataagttgt taaaaaataa
    76861 tggagtaaaa atacaaatcg aagaagagga tgttaattta gatgtaatgg ttacgataaa
    76921 gtatggaatg tcaataccag atacagcttt taaagttcaa gaaaatgtaa aaaatactgt
    76981 tgagacaatg acaggactga aggtttctca agtaaatata cacattcaag gaatcagctt
    77041 taagaaggat aaagttgata aagaagaagc aaaagcaact aagaaaaatt aatattagcc
    77101 ctctgaaaag tcagagggtt ttttgctggt aattgatttt atatggataa ttaaacttaa
    77161 aggagaattt tgattgatga aaaaagatag ggcacaaaag agtacaacaa gagaatatat
    77221 aatgaaactt atataccaaa taaatattaa taaagaagat tttgaaactt tagaagataa
    77281 agtagataat tttttaaaag ataattcaga acatattata aatagatata aagaacttgc
    77341 acttcaatac tcaaaaaata caaatttaaa gttagaagat actgaaattg aagatgttat
    77401 agacaaaaaa tatataaata cagtatgcaa agcattgaaa gaaaatcatg acaaaataga
    77461 tgagttaata aataagcatg ctaaaaactg gacagtagat agaatgccaa aggtagatgt
    77521 ttctatactt agattgtctg tgtgtgaaat actttattta gacactccta ataaagtatc
    77581 aataaatgaa gcagtagaac ttgcaaaaat atattgtgat gataagtctc ctaaatttat
    77641 aaatggtata ttgggaagtg ttgttgatga aattgggaaa taatataata attggaattg
    77701 atactagctg ctatactact tctatagcag ctatctcttt agataagaaa gttatattca
    77761 atgaaaaaat aatgttagaa gtaagggata attcaaaagg tttaaggcaa agtgaagctg
    77821 tatttcaaca tatcaataat ttagggattc ttagtgatag gataaaatct tttaaagata
    77881 agtttaatgt agaaggagtt tgctcatcaa aaaaaccgag acctgttgaa aactcatata
    77941 tgcctgtatt caatgtaggt cataattttg gaaaattatt gtctagtata tatggttgta
    78001 gattttatga gactactcat caagagaatc atattgaagc aagtttatta aatagtaaat
    78061 taaaaaataa taataaattt atttctgttc atatgtctgg tggtactaca gagatacttt
    78121 taacaagcaa acaagatagt catcacaatg tatgtgatac taatttaggt aaaattgcca
    78181 aaataagtat taaaaaagat gataaaagta agttatacaa taattttgga tataatatag
    78241 atataattgg aggaagtaaa gatattagct ttggtcaact tatagataga gtaggaatta
    78301 agcttggata taaatttcct tcaggaaagt atttggatga aaatgcttta aattgtaatc
    78361 ttaaaataga aagtggtctg aaaacttctg taagagatgg gtatatgaat ttatcaggat
    78421 tagaaaatca ggtaaataag attataaatg ataatggaga taatactaat caaaaagaat
    78481 atatatctaa attagtatta gattcagtag ttagaaatat gtttaaatct ctagtgtatt
    78541 tatgtgaaac ttataatgta aatgaagtag tatttgctgg tggagtttct gcaagtaagt
    78601 atattcttag agaattaagc atgaagttaa gaaaaaagca tatagaagct tattttacag
    78661 aaccacaata ttcaactgat aatgctgtcg ggtgtgctat aataggattg aataactttt
    78721 taggagaaag agtatgaaac taagagcttt agatataagt gaagcaaact catatattaa
    78781 aagaatttta ataaacgacc caatactttc taatcttaaa gttaaaggag aaatttctaa
    78841 ttttaaggtt catagtagtg ggaatgtata tttatcactt aaagatgaaa catcaaagtt
    78901 aaactgtgta atttttaaaa gtaattttaa tcgcaattta aaattagata atggtgtaaa
    78961 aattatagct aatggatata tatcagtcta tgaaagagat ggtgcgtatc aattatacat
    79021 taatgaaatt gaaatagagg gaataggaaa tttgcacata gagtttaata gattaaagga
    79081 aaaacttaat aaagaaggat tatttgaccc taaatacaag atacctatac ctaaaatgcc
    79141 aaactcaatt ggcgttataa catctccaac aggtgctgtt ataagagata taataaatgt
    79201 aataaaaaga agatatccga aagttaatat aaagctttat ccagtaacgg ttcaaggaga
    79261 taaatcagct gaagaaatct gtgaagcaat tagatttttc aatcatatga agaatgttga
    79321 tacacttata gttggtagag gtgggggttc aatagaagaa ctttggtctt ttaatgatga
    79381 aatggtagca agagaggttt ttaattctca aattccgata atatcagcag ttgggcatga
    79441 aacggatttt actatttgtg actttgtgtc tgatatgaga gccccaacgc catcagcagc
    79501 agctgagatt gctactccat cattggatga tataaactat aaacttggaa acataaaaag
    79561 taggatgagt aagtctctta caaatcaaat agagctagac caatataggt tagaaacagt
    79621 atttaataag ataaataact atttagattc gtacacaata aaagataaag ttatacaatt
    79681 agacaaaata tatgataaaa taatttttgg aatagaaaat aatttaaaat tagaagatga
    79741 aaaattagta aaaattggag cattactcca taatttaagt ccattagcta ctatggatag
    79801 aggttatagt ataactcaaa aaaatggaaa agtaataaat agtataaaag gattaaaaat
    79861 aaaggatagt atagatatag tattaaaaga tggaaattta gaatgtatga tagataaaat
    79921 tgaaaataaa gagggatagt atgaatttaa cctacgaaga agcttataaa agattagaaa
    79981 gtattttaag tgaattagaa tctaaaaatg ctagcttgga tgaatcttta agcctatatg
    80041 aagaaggtat aagcctttat aaacattgta ataagctttt agatgatgct aaattgaaaa
    80101 taagtaaatt taatcaattg ggaatagaag aagactttaa aatagaggag gaataaaatt
    80161 ggaatttaaa caatgtctaa aagaaaaagc aagttttgta gaaaaagtac ttaaagaata
    80221 tatgcctaaa gaagaagggt atcaaaaaac tgttattgaa gctatgaact atagcttaag
    80281 tgctggaggt aaaagattaa gaccaatact tacattggag gcttgtaaaa tagttggagg
    80341 taatgaagat gaagctattc cttttgctat tgcaattgaa atgatacata catattcact
    80401 aatacatgat gatttgccag ctcttgataa tgatgattta agaagaggaa gaccaacaaa
    80461 tcataaagta tatggagaag ctatggggat tttagcagga gatgctttgt taaactatgc
    80521 ttttgaagta atgcttgcag gctcaatcaa taaagaaaat ccagaaaagt atctaaaagc
    80581 tataaatgaa attgccaaag gtgctggtat atatggaatg ataggtggtc aagtagtaga
    80641 tgtagaaagc gaaaataaac aaattgaaaa agaaaaatta gattatatac atatgaataa
    80701 aactgctgca atgatggtag gctgtatgag agcaggagct actataggtg gagcaaattc
    80761 tgaacaaatg gaagaaataa ctaaatatgc aaaaaatatc ggattatcat tccagatagt
    80821 agatgatata ctagacatag taggagatga agctaagtta ggtaaaaagg ttggaagtga
    80881 tatagaaaat cataagtcaa catacccatc tcttttaggt ttagataaat ctaaagaaat
    80941 tgcacataat ttgattgatg aagcaaagaa aagtatagaa aaattatctg atgatgtaga
    81001 ttttttaaag ggattggctg aatatataat agatagagaa tactaaaata tacagctaaa
    81061 atcgaggagg gaaaaatgga ttttttttcg gagattttta acaatggggc tttgggaata
    81121 agtctgattg cttgtttctt agcacaattt ataaaaatat ttactggtaa ggaaaaaaga
    81181 attgagttat caagaatact tatttcagga ggtatgccta gttcccatag ttcttttgtt
    81241 acaagtttag ctacagtggt aggtattgaa aaagggttta attctactga ttttgctata
    81301 attaccgtgc ttgccttaat tataatgtat gatgcagcag gtgttagacg ggctgttggt
    81361 aaacaagcta ctatattaaa tcaaatggtg gcagatatac agcatggaaa gcatatagag
    81421 cagaagaaat taaaagaatt aataggtcat acaccactag aagtttggtt tggagcttta
    81481 cttggaatag taacagcttt aatattgatg taaattgaaa atttacagaa taaaggtgag
    81541 gataatatgt ataaatattt agataaagta aattctccaa aggatataaa aaatatgagt
    81601 atagaagaaa tggatttact tgcaaaggat ataagaaagt tcttagtaaa atctgtttca
    81661 aagacaggag gacacttagc ttctaatcta ggagtggttg agttaacatt agcacttcat
    81721 aaagtatttg atagccctaa agataagatt gtatgggatg tagggcatca atcttatgtt
    81781 cataaaatag ttactggaag aaaagattgt tttgtatctt tgagacagtt taatggacta
    81841 agtggttttc caaaggagaa tgaaagtcct catgatattt ttgatacagg acatagcagt
    81901 acatctattt ctatagctac aggtattgcc tgtgctaggg atataaaaaa agaaaactat
    81961 agtgttatat ctgtgatagg agatggttct ataacaggag gtatggcact tgaagcttta
    82021 aatcaattag gatatataga taccaatatg atagtaatcc ttaatgataa tgagatgtcc
    82081 atagataaaa atgttggagg aatgtctaaa tatctgtcaa gtattataag aaactcaaca
    82141 gttgagaaaa tgacagatga agttgataaa attttaaatg ttacacaaac aggtgaaatt
    82201 ttatctaaga ctgcacatag atttaaagat aaactaatgt atagcttttc tcctcaggac
    82261 tgttcatttt ttgattcatt aggtataaga tattatggac ctatagatgg ccataatact
    82321 aaagaactaa tagatatatt gagaaaagct aaacacaaaa aagggcctgt tcttttacat
    82381 gtaattacga aaaaaggaaa agggtacaga tttgcagaag aacaacctga taaatatcat
    82441 ggagtatcaa agtttgatat aaagacaggt gttacatctg caaaagttaa gtctatgtca
    82501 attagtgttg gagaaaaact tgttgatatg gctaatagta atgaaaatat agtagcaata
    82561 acagctgcaa tgccatctgg tacagggtta aacctgtttg aaagtgcata tccaaaaaga
    82621 tattatgatg ttggtatagc tgaacaacat gcaacaggat ttgcagcagg tcttgcaaaa
    82681 aatggtatga aaccttactt tgctgtgtac tcatcatttt tgcaaagggc atatgaccaa
    82741 gttattcatg atgtatgcat aacaaaaaag ccagttactt ttcttataga tagagctgga
    82801 ttagttggaa atgatggaga aactcatcat ggtatgtttg atttaagtta cttaaattct
    82861 attccaaata ttgtagtaat ggctccaaaa gacacaagag agatggagct tatgatggat
    82921 ttgtcattaa aattagattg tccactagca attagatacc caagaggaag tagttattac
    82981 ctagataagg gagagtatgg agagattgta ctgggtaaat atgaggtatt agatgacggt
    83041 caagatacag tcatactttg tattggaagt atggtaaaac atgctttaga ggctaaagaa
    83101 atattatcaa gagaaggaat aaatccgaca atagttaatg ctagattctt aaagccaatt
    83161 gatgaaggta tgttaaaagc attattaaaa aatcataaaa atgtagttac tatagaggat
    83221 aatattgtaa ctggaggatt tggaagtaga ataaataagt ttattataga taatgaatat
    83281 aacgtaaata tacttaatat agcaatacca gaagaatttg taaaacatgg caatatagat
    83341 gaattatatg actttgtagg attatcgcca aaaagtattg ctgataagat aaggaaatta
    83401 gttattgagt aaaaatctaa tgtagatatt gtaaattaaa atttatataa aatatagaat
    83461 ttatcaatta gaataaatat caagtgtgtt acttcaagct actttagtta tcattaaagt
    83521 agcttttaac agaaaggtaa ttgtatgaaa aaaagaatag atttattatt agtagaacaa
    83581 gggtactttg aaagtagaga aagagccaaa aaagcaataa tggctggatt ggtttttgtt
    83641 gataatcaaa ggtgtgataa ggctggtaca gaagtaaaag aagattgtag tatagaagta
    83701 aaaggaaatc caataccata tgtcagtaga ggtggtttaa agcttgaaaa agcaatgaaa
    83761 aactttgatt tgactattga tggaaaggta tgtatggata taggggcatc cacaggtggt
    83821 tttacagatt gtatgctcaa aaatggagca ataaaagtat tttctataga tgttggttat
    83881 ggtcaacttg catggaaatt gagacaagat gatagggttg tgtgtatgga aagaaccaat
    83941 ataagaaatg tgacaataga agatacaaag cagtttgctg attttgcgtc aattgatgtg
    84001 tcttttatat cgttaaagtt agtacttcca aaggcaaagg agttagtacg tcatgatggg
    84061 gaagttgtcg ctttaattaa acctcaattt gaagcaggaa gagaaaaagt aggtaaaaaa
    84121 ggagtagtta gagaaaaatc tacacatata gaagttataa aaatgatttc tgatttctca
    84181 gtcgaaaatg gatttgagat tttagggtta gatttttctc ctattaaagg tcctgaaggt
    84241 aatatagaat atctaattca tttgagaaat ggaaatgaag gatatgaatt tgatggtgag
    84301 acatacaata acaaaatagt agaagttgtt gaagcttctc ataatttaga caaataggtt
    84361 aaaagggggc ttagatttga tcctcgaatt atatatgaaa aattgtgcct tagttgaaga
    84421 actaaggttg aatattgata aaaacttaaa tatacttaca ggtgaaactg gttcaggaaa
    84481 atctataata atagatgcac ttggactttg tttaggtgat aaatatgata ggtctttttt
    84541 aagaaaagga acagataagg gacttgttga ggctgtattt ttttcagaca atagatatct
    84601 taaaaaaata ttagaagaaa atgatataag tatggaagat gataatttat tggtaataac
    84661 taggttgata tactcagatg gtaagagtac tgctagagta aatggaagga ctgttaaagt
    84721 gtctcttctt aaagaaattg catctacact tatagatata catggtcaac atcaaaatca
    84781 agctttgttt aataaagata ctcatttaaa atttctagac ctttttggag aaaatgaatt
    84841 ggaagagttt aaaattgctt ataaaaatgt gtaccataag tatagtgaag taaaaaaagc
    84901 tttaaattgc ttgacagaaa ataaagatga gatgcaaatt caacgagaaa tagatttatt
    84961 aagatttcaa ataaatgaaa ttgaagctgc aaatctaaat aagaatgaat atgaagattt
    85021 attaaaacaa agagaagttt atagaaatag tgaaaagata tataataatc taaattcgag
    85081 ttatagcaag ttacataatg gtgagtataa tgttattgac ctaataggtc ttgcatctaa
    85141 ggaacttaat gacatatcta aatatgacag tgttttaagt gaatatagtg atactgtaga
    85201 aagaatcatg tatgagttac aagacatatc tggtgagata agaaattaca aagataatat
    85261 agactttgaa ccttatgagc tagagcagat tgagcttaga atcgacgaga ttaataactt
    85321 aagaagaaaa tatggagaca gtatagatga tatatttgag tattatggaa aaattaaaga
    85381 tagattagat gaaatattaa acagagatga aagagttgaa cagttaagaa gcaaactgat
    85441 gaatatagaa gaagatttaa aaataaaagc cagtaaattg actaaagcaa gaatagaagt
    85501 agcaactact ttagaaaaaa tacttcttga tgaattaaaa agcttaaata tgaaaaatgt
    85561 aatgttcaag gttaattttg aaaaaggtcc atttacttta gatggagtag atgatattga
    85621 atttatgata tcttttaact taggagaaga tattaagcct atatataaag ttgcatcagg
    85681 tggtgagatg tccagattta tgttagcgtt taaaaccata ttggctgata tagatgatat
    85741 tgatacatta gtgtttgacg agatagatac tggaataagt gggatagcag cacagatagt
    85801 tggtgagaaa ttgagtgata tagcaaagaa aaaacagata atatgtataa ctcatttacc
    85861 tcaaattgct gcaaatgcag acactcatta ttgtatagag aaagatactt caaataatag
    85921 gacgtttacc aatgttagta agttaaatga atctcaacga aaaaatgaaa ttgccagact
    85981 tatagcaggt aacaatataa cagaaaaaac tattgaacat gcaagtgaaa ttattgagtt
    86041 ggctaaaaaa tgttagtgaa aaacaagcat tttaataggt gtaaagaatt tttatagaaa
    86101 tgctttacat ctatttttta tgatgaaatt attttaaaat atctagtgcc tatattgaag
    86161 aaaagtaggt ttgaaaatgg aatgaacttt gtataaattt gtaactttga aatttctaaa
    86221 tgcttaagta taatattatt tattaaggta gtagtgaggg gaggcataaa atgaataaaa
    86281 aagatactat gcacttaatt tttataattg taatttttat tttattatgg tatattgtgg
    86341 tagcagtaag tgagattaat aaaaatattg aaagttgtag taacaaatta gattatttgt
    86401 cacaagagct tatgagtact aagtcggata ttagtaatac tataaatgaa gaaatgtcaa
    86461 aaagctatat aacaaaaagt attgacttaa aagtgaaaaa aatcgagaaa aaagaatgta
    86521 ttattgatat agatgttcaa ttaagtaaac ttggaaaaaa tggaaaagta ttttttatgt
    86581 ataaagaaga tagtgataaa tggaatgaaa ctgagatgac aaaacatgga gaattatcat
    86641 atgcatgtga aattaagata aatacaggaa gtgaatatga ctataaagta gttacaagtg
    86701 gagctatatc tgaaagttct gatgtgcaaa aattgactaa atctgattat atgcctgaac
    86761 aacccatatt taattctggg ataagagata atagagaata ctttattgaa atagtagaaa
    86821 atagtaatct tttcaatcat gagagtgaaa agtcaaaaaa taaagtttat gataatatta
    86881 gattagaaaa agtagatatt attgtaaatg aaggaaaaaa agacactata tacaaagcca
    86941 aattagctga aggcttagat gatggcaaaa ctaataattc aaatagaaat caagtgaatt
    87001 atgaagtaag ttttcctaaa agggatattg atgacataat ttatattaaa gctaaactta
    87061 catacaataa tggagtagaa tatacaaagg atataactga agatataact atgggattac
    87121 aagatttaga aaaataaata catatttaaa taaatattca aatagatttg ttcaataaaa
    87181 tagagtataa ataaaactgt agtagtttta cttatactct attaaaaaat tataatttta
    87241 attccatata tctagcccct tcaattggat tataagtata aggttctata tcataaaaac
    87301 caaacttttc gtataatcct tgtgcacttt tcatgcttgg aagagtatct aatctcatgt
    87361 gtgtatatcc aaatttttta gcttcttcaa tgatttcttc caatagtatt tttcctattt
    87421 ttaatcctct aaattgattt cttacatata atctttttaa ttcacatatt ttaccttcaa
    87481 gtttttttaa agcaacacaa cctgccaaat tttcatcaac aaatgccaat atcaatgagc
    87541 ctgaaggttt tttatatttt cctggaagtg tttttaattc attattaaag tcttgaaaac
    87601 ataagtctat atttagagaa tttgaatatt cagtaaatag aagttttacg ttttcaaggt
    87661 tttctaaatc tttttcttca acaaattgta aagttatatt ttgagccatt ttatccgcca
    87721 ttatatacac atgtttatat aatgacttca cctccttgat atatgtatat ttttcttaat
    87781 atcaattaga ttttaatacc attacttttt caccaccata gtcaagctct ccattaaact
    87841 caaatccaaa atctttataa agcttaatag ccattttatt gtcttcgaat atactaagat
    87901 atagttcatc acagttatat tcatttttaa gatgatttac aagaaaatct aatgatgctt
    87961 ttccatatcc tttaccttga tattttgaat caatcataaa tctatcaagc caaactctat
    88021 catcttctaa acgaccatac atagcaaatc ctattaaaga attttcatca tatattccaa
    88081 caggtaccca agctgttata aatttagatt cagcaataga aagtgcattg ctttctatat
    88141 aatctaactg acttttatct acagacaaac taactaaatc atgccaatta tttatgtctg
    88201 caattcttac atttaatttc aagatattac cgcccttcaa actttttgtt acaatctaat
    88261 actatcatat tatgtattaa aaatcactta ttaagatttt aaataaactt tttaaatgaa
    88321 taaattctca ggaaaaagga attataaata cataataata cagcatagga gtagatggat
    88381 atatgaacaa aataaataaa aatttcaaaa atatttggat aatattagga gctgtatcga
    88441 tttttaccat ggttataatt tttaataaaa attgtaatgt taataaagag actcaaatag
    88501 aagcttttac aattaagaat catgataaaa aatttgtata tcctatggga aatataattg
    88561 gagtaaaagc aaatacagat ggtgtattgg tcttaggata tgaggaagag gatgtagact
    88621 atattggagg catacaaatc ggtgataata tagtaaaaat agataataaa aggataaaaa
    88681 atagtcaaga tgtatcagag attttgaata agattaaaaa aagtaaagtt gaagtgactt
    88741 ttgagagaaa aaataagtat aaaacagaag taatagagac taaaaaagaa aatggcaagt
    88801 ataaacttgg attatgggta agagataaaa tatctggaat tggaactatg actttttacg
    88861 accctagtat ggaaaagttc aaagcaattg gtcatgccat aaaagattcg gatactaatg
    88921 aactattaaa gataaagcaa ggatatatat ataagccaga acaattaaaa atagtaaaag
    88981 ctagtaatga aaaagtaggt aaaataaaag gagattttaa tgacagcaat ttaatgggta
    89041 atttctcaaa taattcagag ttaggtataa gtggtaatat tacagaaaac cataataaag
    89101 agtttaatgt agcaaataaa gaaaagcaat taatagaagt tggaagaccg caagatgtaa
    89161 aaataggagg tgcagtaatt ctttttgagg ataaaaacaa aaatataaca agctatgata
    89221 taaaaataga aagtatagta tatgataaag gaaattatag agatatggta ataaaagtag
    89281 tagatgacaa gttattggaa tacacaggag gtatcgtaca ggggatgagt ggagctccaa
    89341 taatacaaaa taataaaatt attggtgcaa taactcatgt ttttagagat aatccgaaaa
    89401 aaggatatgg tatttttata gatgaaatga taaaattgta ggtgaggcat taaaaaattt
    89461 tattatttta tcaattatct aggaggaata taattttgga gtgtcgaata tgctttagag
    89521 tagataatta ggaagcaatt gtgtaaaaag tttagttttc tgtaataaga agatgttttt
    89581 taatgggggg atttttagtg gaaaaaatca aaatagtttt agcagatgac aataaggatt
    89641 tttgtcaggt attaaaagag tatttgtcta atgaagatga tatcgatata ttaggcatag
    89701 ctaaggatgg aattgaagca ttagacttag taaaaaagac acaaccggac ttattaatac
    89761 tagatgtaat aatgccacat ctagatggat taggtgtaat tgaaaaattg aatactatgg
    89821 atatacctaa aatgccaaaa ataatagtac tatcagcagt aggtcaagat aagataactc
    89881 aaagcgcaat aaatctagga gcagattact acatagtaaa gccatttgat tttgttgtgt
    89941 ttataaacag aattagagag ctagtttcta atagagttac acaagttgag ccaaaaccta
    90001 gaccagttca agagactcaa atgactagaa gtgattttgt taagaatgta ggaaatatag
    90061 agacagaaat cacaaatata atacatgaaa taggagtacc agctcatata aaaggatatt
    90121 tatatttaag agaagcaata aaaatggtta ttgataatgt tgagctttta ggtgcagtaa
    90181 caaaagagtt atatccaagt atagctaaaa aattcaatac aacaccaagt agagttgaaa
    90241 gagcaataag acatgcaata gaggttgcat ggagtagagg aaaagttgac acaataaatc
    90301 aattatttgg atatacggta cacaatacta aaggaaaacc aactaattca gaatttatag
    90361 caatgattgc tgataaatta agactagaac atagtatggt taaataaaca agacataaaa
    90421 agtaaggctt tttaattaag gcattggcta taaatgcgta ttacaagcag cgaaacggta
    90481 taaccactag ggttataccg tttcgctatt ttaataaata taaaaatttt atttattatt
    90541 tgcttactat atcaatataa taattttatt atactatgga tatagtatgt gtctttacaa
    90601 gttgtaaact gacagtggtt tattttttaa tataaatatt gactttgatg caggtaaact
    90661 ttgtattttt aagcgtatta tggaatatgt taaataaaaa aatgatgaaa tatagtattg
    90721 taaatgccaa agatgcaaaa caaacttaaa acatttattt tattgttaag taatgctata
    90781 atataatgtg attttaataa tgatagtgga ggtttaaata tgagagtcga ggcccctata
    90841 aaagtagatc gaaaaaccaa aaaacttgct aaaagagttg aaagtgggga aatagcagtt
    90901 ataaatcata tagacataga tgaagttgct gcaaactctt tagtagaagc taaaataaaa
    90961 cttgtcataa atgcggctcc ttctataagt ggtaggtatc ccaataaagg tccagggata
    91021 ttaactgaaa ataatatact aataattgat aatgttggag aagagttatt tgaagaatta
    91081 aaagaaggag aaactataga agttgtagat ggaaagatat atagaaaagg aaaattttta
    91141 ggtgcaggag aagttttaga caagtatgag gtttcacacc aaataaaggc tgcctatgag
    91201 aatttagctg tagaattgga tagatttata gataatacta tagattatgc taaaaaagaa
    91261 aaaggattta tacttggaga agtagaaatt ccaaaagtga aaactaatta tgctaataaa
    91321 catgtattga tagttgtaag aggtcaggac tacaaagaag atttaagtac tatgctttct
    91381 tatatagaag aggtaaagcc agtattggtt ggtgttgatg gaggagcaga cgctttaatt
    91441 gagtttggat atactccaga tgtgattgta ggtgatatgg atagtgtaag tgatgaagct
    91501 ttaaaaaagg ctagtgaaat agtagtacat gcttatacag atggaagagc accaggtctt
    91561 aaaagggttg aggaattagg cttagatgca gttgtatttc ctgcaccagg aactagtgaa
    91621 gatatagcta tgcttatcgc ttatgagtat aaggctgagt taatagtagc agttggtaca
    91681 cattcaaata tgattgattt tcttgaaaaa ggaagaaaag gtatggcaag tactttttta
    91741 gttagactaa aaataggttc aaagcttata gatgctaaag gcgttaattt gttatacaga
    91801 agtaaattaa aaataaagta catatgggca ttaatagcaa cagcattatt tcctgtgttg
    91861 gtagttgcat cattgtcacc aggagttcaa cagttcatac aactaatgca attaaagttt
    91921 agagtattat tacagatgta aattttagga ggataaatat gcatattaat atgaaatatt
    91981 atatagtcac tataggggct atatttatag ctttgggtat aggcatgtta gtaggattta
    92041 atttaaataa taatcaacaa ttaagtgaac aacaagcaaa cataataaat gatttggatg
    92101 ataagtttga tatcttaaaa gaaaaaaatg ataaacttga cagtgactta gcaagtgtaa
    92161 atagagatta tgaagaggca gttaatttta taaataaaaa tgtagataag attttagctg
    92221 gttcgctgaa tggtaaaagt ataggaataa tatctgctaa tgaaaatgac gattatacaa
    92281 aaaatataga agacataata aataaatcta atggaagcat tgcatttaat ataatattaa
    92341 aagaaaacat aacaaatcca gaaaagttaa aggaaatgtc aactaaacta ggtacagatg
    92401 taaaaaatgc aaatgatgca gttaattata taattgacac tcttaaaaag gaagatgcaa
    92461 gtgacatttt aacatatctt caagaactag atgtaataaa gtttaatttt attggagata
    92521 catatctaaa atatgattcc gttgtaatag ctggtggaaa tgatgcaaaa gacagtacaa
    92581 aacagtttga aaaaatagaa aaatttgtag tttcaaaact taaatcagag aataaatatt
    92641 tagtagaagt tcaaaataca ggagttaaaa cttcttatgt agaactatat tctaaaaaca
    92701 aagttgcaac tatagacaat attgatgaag gtataggaag tatatctcta gctattcttc
    92761 tacaacaagg taatatagtt ggtaattttg gtagattgga tactgcaact tcattgttgc
    92821 catctattaa atagaaataa ttttaaggag agaaaaaatg gattcttata taagtataat
    92881 aataccagct tataatgaag aaagtagaat aaaatataca ttagaaagca tattggacat
    92941 taagcaaata aatgaaataa tagttgtgga tgatggttca attgacaata ctgctaaggt
    93001 tgtatctgag ctagaaaatg aaaagattaa attttttaag ttagataaaa atagaggtaa
    93061 aggatatgct ttaaattatg gtatcaaatt agcaatgaaa aatgcagata ttataggttt
    93121 tctggatgga gatttaggaa gttctgctaa agaagttgaa aaattaatag tacctatatt
    93181 aaatggtgag gcggatgtta caatagccaa gtttcctcct gcaaagagaa aaggaggttt
    93241 gggatttgta aaaggtttag ctaaaaattc tgtgtttgag atgactggtg ttgagttaga
    93301 tgcaacttta tcaggtcaaa gaatatttaa aagagaagtt ttagaaaagt ttgatgaaat
    93361 accttttgga tatggagtag aagttggaat gaccattgat atattaaagt atggatttaa
    93421 aattaaagaa gtattagtaa atatgactca taatgaaaca ggaagaaatt taaaaggttt
    93481 tattcataga gggaaacagt attatcacat aaaaaaggtt ttaggacaaa aaaagaaaga
    93541 atggaggtaa tatcttagtg aaagattata tactttatac cattttatta ttaactggat
    93601 tatttggaac ctatgctgta attccactat ttaaaaattt attgataaat ggaaatgtac
    93661 ttagaccaaa ctataaaaaa gatatgatac cagtaagtat gggaattgta tttttgccta
    93721 tgattgtaat caatggaata atacttgctt tctttactac agaatttaag gatttagcgt
    93781 atatatttat gtttatgttt ggtatgatat ccatgttttt tgcagggata ctagatgatg
    93841 tcattggaaa tagagatgta agtggtttaa aggggcattt taaaagttta tttaaaggaa
    93901 gtcttacaac aggaggtttt aaagctttat ttggtggatt tgtagggctt atagtttctg
    93961 ttgctatatc aaaagacata attgatataa tagtaaatac actaataata gctctatcaa
    94021 ctaatttaat gaatcttttg gatttaagac caggaagggc aataaaagca tatttattca
    94081 taatggtaat tatattttta acattgacag gatttgttca agttcttcca ttacttatag
    94141 taccaaatgt acttgcatac tttaattatg atttaaaagc aagggctatg atgggagata
    94201 ctggttcaaa cgtccttgga atatctattg gaatgctcat ttcttttggg tatccattta
    94261 acattagatt aggatggtta atatttgtag ttattatgca tatattcact gagaaatact
    94321 cattaactaa gataatagaa aaaaataaac tattaaattt tatagataaa ttgggaaggt
    94381 agtattatga taagcaaaag agtggggatt gttgaatcaa tagtaagtca aactgaaaca
    94441 ctagatgata taagagtaaa tataaatgga gaaatacaaa gggcatataa ttatcccaaa
    94501 atatctggaa ctataaatat aggtgacgaa gtggttttaa atacaacagc agtagaactg
    94561 agcttaggta caggtggata ccattttgta ataactaatt taaataatat agaatctact
    94621 ttaactgaag gtggacatat aatgaaactt agatatacgc ctcttcaaat aaaagtggat
    94681 tctgttgagg aacaagaaag tatttatcat gataaatttg cgaattttaa aggattagaa
    94741 ggtctttctg ttgtagtagg gactcttcat agtatgttaa ctccatttgc agcttcattt
    94801 aaaagaaata atccaaataa aaaattagtg tatataatga ctgatggagc atctcttcca
    94861 atttatttaa gtaaaaatgt ggatacttta aaagaaaaaa aattaataga tagtacaata
    94921 actataggaa atgcgtttgg tggagattat gaatgtataa atatatatac tgcattaatt
    94981 acagcaaaag aaatactaaa agcggatgca gtatttgtca gtatgggacc tggaattgct
    95041 gggacaggaa caaaatatgg atttacaggg attgaacaag gctctattct tgatgctgta
    95101 aaaaaacttg aaggaagagc aattgcaatt ccaagaatta gttttgctga caaacgagaa
    95161 agacatcaag gtatttcaca tcatagtatg actgtattta aagaaattgt aaatgtaaat
    95221 gtagacatac caataactat atatgatgat gaaaaattga actttataaa agagcaaatc
    95281 aatttaaatg gtttagacaa aaagcataat ataatttata tagataataa taaaacgaaa
    95341 gaggatttag actattttaa tctaaaagta aagagtatgg gaagaaatta tgaacaagat
    95401 gaggcatttt tcaaagctgc aagcacagca gcatattact taatggaggt ttaagatggt
    95461 attagaagaa aaaactatta gtagtgatag ggtttataca ggtaaagtaa ttactttaaa
    95521 ggttgatact gttgaaattc cagggcaagg atatcaaaaa agggagttag tagaagttgg
    95581 tggagcagta ggaatagttg caatcacaga tgataataaa gttgtgttag ttaagcaatt
    95641 tagaaaacct atagaaaagc caatctttga aattccagct ggtaaactag aaaaaaatga
    95701 aagtccaaaa gaatgtgctg aaagagaatt aaaagaagaa acagggtata gtgcaaaaaa
    95761 tattaagcta atacataaat ttttcacttc agcaggtttt tctaatgaga taatgtttgt
    95821 atatttagca acaggtctta ctccaggaga gaataattta gatgcagatg agtttttaga
    95881 tgtctatgaa attgagcttg aagaagcata taacatggta ttaaaaaatg acgttgaaga
    95941 tgcaaaaaca tctattggat tattattagt aaaagatatg tttaaaaatt aatatttata
    96001 aaatgaataa ccctttgtcc taaagcatat tattttataa aggacaaggg ggaattaaat
    96061 tgagaagaac atatagaaaa aatgatttta gagaattaaa tagacaaata attattatag
    96121 gattgttgtt tatgatgtct attgttgttg ggtcttacat aaataaaata ttgccaggtt
    96181 ctagtaacaa tatattaaac aatataaatc ctgctgtaga gtattataat ctaaatataa
    96241 gcataaaaga tacagtgata caaaatttaa aatcagatgc aatctttatg ggctcaatag
    96301 ctctattaag tctatttgta gtaacaattc cagcagtgtt ggtagctttt gtattaaaag
    96361 ggatgtcaat tggttataca ataaatagtt gtatattggc attaaaattt aaaagtataa
    96421 aaatgatttt aataatatta tttaaaaatt tgattattat tccatgtgca atcatattag
    96481 cattaattag tttaagttat tttaaagaaa tggtttatga atttaaaaag aaaaatagaa
    96541 aaaatatgca atttttaatt aagaggtata tactaaatat tataattata atagcactat
    96601 cattggggtt gcaattaata ttaaatactg caagtattgg tattattaag ttcctagcta
    96661 aataaaggag tttataatgg atattataga gggatatata gattacataa aagataaaaa
    96721 aaaattatca gagaatacag tggcatcata ctttatggat ataaaaaaat atatggaata
    96781 tataaatcaa aaaagcatta aactagtaga tatagtagaa aatgatatta taagttactt
    96841 aataaagttg gaaaaagata atgtatcaat agctacaata gcgaggatga tatcctctat
    96901 aaaatctttt catgattatt tatttttaaa tcatatatgt acaaataatc ctgcaaagga
    96961 tataaaaaaa cctaaaataa aaaaagaaaa tataaatata cttacagaag aagaaattga
    97021 aagactatta aattttccaa agttaacaac acctaaattg attagggaca aggctatttt
    97081 tgaagtatta tatggtactg gtataaaagt atctgaatta gtagagatga atatagagga
    97141 tattgaccta gatattgatt atatatattg taattcatgt tgtaaaaatc aaagagtgat
    97201 accactttgt gatattacaa aactttactt agaaaagtat ctaaaagaag caagacctaa
    97261 aatggctgta gaaggtgaaa aatcattatt cgtaagttca ttaggtcaaa ggtttacacg
    97321 acaaggtctt tggaaagtca taaaaaaata ttctaatcta gccaatatag ataaaaatat
    97381 aaatcctaca atgcttagac attcatttgc tattcatttg ttaaatgaag gtgctaacat
    97441 tgctgttgta agtaaaattt taggaaatgt taatttatct agtttgcaag tatatttaaa
    97501 tcatatagat aaaaacgtga gaagagaaat aaaggagaaa catcctagaa atgatgttga
    97561 tgtagaatta caaaaagcag aagctaaata gactatatag caaattttat aatttagaag
    97621 gagcatattt atatgagcag agtaatttgg attgtaattg atagtgttgg gataggagca
    97681 ttgcctgatg ctgaaaaatt tggtgatagt aaggatgtaa gtacccttgg aaatatattt
    97741 aaggaatatc cagatattca gattcctaat atgagaaatt taggtatagg aaacatagat
    97801 ggtatagatt tttttgaaag tataaaagaa ccaataggtt gttttggaaa gtgtaaggaa
    97861 atgtctcagg gaaaagatac tactacaggt cattgggaaa tgactggtat aatagttgac
    97921 aaaccattta agacatttga acatggattc tctaaggaga taatagaaga gtttgagaaa
    97981 aaaactggaa gaaaagtagt aggaaataaa ccagcttcag gaactgtaat aatagatgag
    98041 tatggagaac atcaaataaa aactggagat gtaatagtat atacatcagc tgatagtgta
    98101 tttcagattg ctgctaatga agaagtaatt cccctagaag aactctataa tatgtgcaaa
    98161 atcgctaggg aaataatgat gggggataat gcagtagcta gagtaattgc tagaccattc
    98221 ataggtaaga aaaaaggtga gtttgtgagg acttcaaata gaagagatta ttcactagac
    98281 ccatttgaac caacagttct tgataatata aaagaatcag gacttgatgt tttagcagtt
    98341 ggaaaaatag aagatatttt taatggaaaa ggaattacag atgctatcca cactaaaagc
    98401 aatatggatg gagtggatga aactttaaat tatatgaaac aagataataa aggtctaata
    98461 tattcaaatc tcgttgattt tgattctaaa tatggtcata ggagagaccc agaaggatat
    98521 aagaaagctc ttgaagagtt tgatagtaga cttcctgaaa taatggctaa tatgagagaa
    98581 gatgatattt taataatcaa tgcagaccat ggaaatgacc caacatataa aggtacagac
    98641 catacaagag aatatatacc tgttatgatt tatggcaata agattaaaaa aggttttaat
    98701 ttaggagtaa aagatacttt tgctgatatt ggagcaactg ttgcagatat actaaatgta
    98761 aagttgccta aacatgggtc aagctttaaa gaagatttat tttaaactat aaaatgataa
    98821 aaacttagct taataggatg gagagattgg aatgtttgaa aagatagcac aaagtagtaa
    98881 atttataaat tctaaaagtg atataaaacc taagattggt ttgatattag gttctggact
    98941 aggtgattta gcaaatgata tagaagaccc tgtcgcaatt aaatatagtg aaattccaaa
    99001 cttccctgta tcaacagtag caggacatgc tggacaactt gtaattggaa aacttgaagg
    99061 aaaagaagtt attgctatgc aaggtagatt tcattactat gaaggatatt ctcagaaaga
    99121 agctactttt ccagtaaggg ttatgaaaga attaggagtc gagattctta tagtaactaa
    99181 cgctgctgga ggcgtaaata aagaatttaa agcaggagat ttgatgctta taagagacca
    99241 tataaacttt agtggaagta atccattagt aggaaaaaat gatgacagat ttggtgctag
    99301 atttccagat atgtcagatg catattcttc taaatatgta gatgtagtta aaaactgcgc
    99361 taaagaatgc aatatagatg tcaaagaagg cgtttatatg ttttttagtg gtccaaacta
    99421 tgaaacacca gcagaagtga gaatggcaca aattttggga gctgatgctg taggtatgtc
    99481 tactgttcca gaagtcatag tagcttctca ctctaatata ggagttattg gtatatcttg
    99541 tataacaaat atggctgctg gcatcttaaa tcaaccacta agtcatgagg aagtaataga
    99601 aactactcaa aaagttaaat cagaatttat gaatttagtt aaaacaacgg ttaaaaattt
    99661 ataagttaaa gcttattata gattttttga ggaagtttat aataaaatag ttaatgttta
    99721 atttgaaaag aattaataaa aagctgtctt gatatagaaa gttatttcta ataaaggaca
    99781 gtttttttat aatactacaa catttgaaag tgttttaaag tttaatattt atttaaatta
    99841 ttataggtta gaatggagat ttcaattatg agaatgtatg atattataaa aaaaaagaga
    99901 gataacctag agcttagtaa aaaagagata aactacttta tagaaaacta ttcaaaggga
    99961 gatatacctg actatcaagc ttctgcacta ttaatgtcga tttatttaaa taagatgaat
   100021 aaacaggaaa ctgtacatct tacagaagca atgatgaact ctggagacat gataaaccta
   100081 tctgagatag atggaataaa ggtagataag catagtactg gtggtgttgg agataagact
   100141 accatagctc ttataccttt ggttgcttca tgtggagcac cagttgcaaa gatgtcaggt
   100201 agaggtcttg gtcatactgg tggtactatt gataaattgg agtctatacc aggtttttcg
   100261 acagaaatgg agataagtaa atttattgat tctgtaaata agtctaaaat agcagtttgt
   100321 ggtcaaagtg caaaggtagc aattgcagat aagaaaatat atgctcttag agatgttaca
   100381 gcaactgttg ataatatatc attgatatca tcaagtgtaa tgtctaagaa gttagcttca
   100441 ggagctgatg caattgtgct agatgtaaaa actggtgatg gagcatttat gaagacatta
   100501 gaagagtctt ttgagttagc taaatctatg gttgatatag gtactggtat gaatagagat
   100561 acaataggga ttgtaacaga tatggatgaa cctttaggtt ttgcagtggg aaactcttta
   100621 gaagttatag aagcaattga gacattaaaa ggaaatggac ctaaagacct tgttgagcta
   100681 tgcgaagtat taggagcata tatgttggtt ttatcaaagg tagcacatga ttttgaagat
   100741 ggtgttgaaa aaataagaga atcaataaaa tctggtactg ctatacaaaa actcaaagag
   100801 tttatagaga atcaaggtgg taataaagat gtagtagatg attattcttt attcaaaaaa
   100861 tcaaagtata aatatccagt taaatcaact aaatcaggtt atataagcaa aattaaagct
   100921 gaagatgttg gtgtatcggc tatgattctt ggagcaggta gagaaactaa agatgatatt
   100981 ttagatttat cagcagggat aattttagaa aagaaagtag gagattatgt aaatgaaggt
   101041 gaagttttag cttacatgta ttataatgat gaacaaaagc tttcattggc aaagggaaga
   101101 tttattgatt cctactctat tgtagattca aaaatagaaa aaaataaatt aatatatggt
   101161 attgtaacta aagaagagat taaaaagttt tagaaaattt tagaaaagtg attataaaag
   101221 gcctaacatg tcaaaaataa tgttaggttt tttatttttt gatagactat aaattatacc
   101281 aaaaatatat attttaaaac attttttaca atataaataa gtttattcta caagatgatt
   101341 gaaaattaaa gtatctagtt ttataataga aaatggataa atatacataa ggagtagatt
   101401 atattatgaa ctttaaagaa aatagattga aaatagcagt attaataatt gttattttga
   101461 ttatcttagc aggtatattt gtgtttatac aaataggtcc ttatgataaa aataacaaaa
   101521 aagatgtaat tatcgacgtt ccaagtggag catctgttgg aaaaatatct gacatattat
   101581 atgaaaataa attaataaag aatgaactat tatttaaact tttggtaaag gttagcaata
   101641 aagctccgtc tataaaatca ggaacatact tacttaatca atcgtattca aacaatgata
   101701 taataagtct tttagtttca ggtaaaatat atcaggatgg tataaaagtg actattccag
   101761 agggagctac atctaaagaa attatagcaa tgttagtaag taaaaattta ggagataaag
   101821 ctacttttga aaatttaata aaaaaacctc aagaatttta tgataaattt ccatatttga
   101881 aggaagatgg aataacctct ttagaagggt tcttgtatcc agaaacttat tattttaatt
   101941 caaagaaaca atcagaagaa gatattttaa gcgaaatgtt aaaagtattt gacagtaaat
   102001 acacggacaa gtttaaaaag aaacaaaaag aattaaatat gactcttcaa gaagtaatgg
   102061 aaatggcatc aataattgag aaagaagctg ttcttgacaa agacagacct ataatagcaa
   102121 gtgtgtttta taatagactt aaggtaggta tgcctcttca atcagatgct acaatacaat
   102181 atatatttga agagagaaag aaaattgtaa catatgatga tttaaaaatt gattcaccat
   102241 ataatagtta taaaaataag ggattacctc ctacaccaat atcaaatcct ggtataaaat
   102301 ctattgaagc agcattatat ccagagaaaa cagactattt atattttgta gctaagattg
   102361 atggtggaaa taattatagt acaaactatc aagaccattt aaaatacgtt aaagaatata
   102421 aagaagcaag ggataaacaa tcaaaagata caaaagcaac aaataaagaa aatacaaaaa
   102481 aataaaccat gattataaaa agtctaacag ggtaagaata atgttgggct tttttattga
   102541 aaattgttat tcttagattt ataataaaga aagtgtaaat atatataaca atatgaattt
   102601 aaaaaataat aaattaatat aaaacttgaa tatttattat tttagggagt gtttttatga
   102661 gtaatatagt taatgatttg gttgaagatt atataagagt gacattaaaa gaaaaagaag
   102721 gatttttaaa agatttagaa atttatgcag aggaaaatag tgttcctata atacataaag
   102781 aagtttcaga tttattaaaa gtcttgttaa aggttcaaaa accaaagaga attttagaag
   102841 taggatgtgc aattggttat tcttctattt tttttgcaac tattatagga aaagatgcag
   102901 atataataac tgttgagaga aatgaaaaaa tgatagaaaa agcaaaagaa aacataaaat
   102961 tagctggatt tgacaataac ataactatac tagaaggaga tgcagaagaa aaacttagtc
   103021 aagtccaagg tgaatttgac cttatattta tagatgcagc caaaggtcaa tataagctat
   103081 tttttgattt agttatagat aaattaaaag atgggggact tttagtatct gataatattt
   103141 tgtataaagg tatggttgct catgatgatt ttgttgtaag aagaaaaaag actatagtta
   103201 aaagaatgag aaattattta gattatatat gtaattgtga ctatttaagc acatcattgg
   103261 taccaatagg tgatggtgta gcactaagtt ataaagaaag taaaagaggt gatgtagatg
   103321 gaattaaata aagtagaact tttagctcca gctggtgatt tggagagatt gaaaatagct
   103381 ataacatatg gagcagatgc cgtttatata ggtggagaaa tttttggaat gaggtctgca
   103441 gcgaagaatt ttagtaagga agatatggca gagggagtag cttttgcaca tgaaagaggc
   103501 aagaaagtat tcgtaacagt aaatataata cctcataatg aagattttct acaattagaa
   103561 gactatttgt tggaactaga agagatagga atagatgcag ttataatagc tgacccagga
   103621 gtgcttagtg tcattaaaaa agtaattccg aatatggaaa tacatttaag tacacaagca
   103681 aatacaacca attatctaag tgcaaacttc tggtacgagc atggaataaa aagagttgta
   103741 gtggcaagag aattatcttt tgatgaaata tctgagataa gagcaaaaac acctttagat
   103801 atggatatag aagcgtttat gcatggtgct atgtgtattt cttattctgg cagatgttta
   103861 ataagcaact atatgactgg aagagatgca aacaaaggtt cttgtgctca atcttgtaga
   103921 tggcaatatc atttggtgga agaaaaaaga cctggtgagt attttccaat atacgaagat
   103981 gaaaggggta cattcttctt taactcaaaa gatttatgta tgatagagta tataccagag
   104041 ttaatcaaat ctggtataac aagtttaaaa atagaaggta gaatgaagac tgcatactat
   104101 gtagctactg ttgtaagagc atacagaatg gcaatagatg aattttacag agacccagaa
   104161 aactggaagt tcaatccaat gtggatggaa gaactaaaaa aaggtagtca tagacatttt
   104221 acatcaggtt tttacttgaa taaaccaaca acagaagacc aaaattatca atcagcatca
   104281 tatgttagaa actatgattt tataggtata gttagagaaa cagaagatga agatggtctt
   104341 attgttgttg agcaaagaaa taaaatgtgt gttggtgatg aaatagaagt tatgggacca
   104401 tataaagaaa ctatgtttac taaaattgaa gaaatgtaca atgaagaagg agaagctata
   104461 gaatcagctc ctcatcctag acaaatagtg aagttaaagc tatcagtaaa agttggtaaa
   104521 gattatatgc ttagaaaagt tatagaagaa aaggttgaag aataataaaa aattgataaa
   104581 aatagtatta gccccttaga aattgtcaat aatgaaatta tctaaggggt gatttcatgt
   104641 caaaaaagaa gactccattt ttgaaaaaag tgggaaagag aagctggtgt atatttacta
   104701 taattttaat aatttatagt gttttaattt ataggctagt tgatatacaa gtattaaaag
   104761 gtgataaata caaacaaagt gttgaatcac aaagcgttga gaaagtcgag ttaaatagtg
   104821 gaagaggaat aatctatgat agaaacaaca agaagcttac agatacaagt aaatctcagg
   104881 tattaattgt tgaaaaagaa aaattaaata ataactataa aattttggag cttattaaga
   104941 aagctactaa gatgaatgat ttagatatat ataaagctgt acaagagcaa ttgacacgcc
   105001 ctataataca gattcagact aaaaatatcg ataagagtat gaaaaaagaa cttgagaaaa
   105061 atggaataat ggtagaagaa aaaactatga ggtacgcaaa agatggttta ttatcacata
   105121 caataggata tataaaagaa gatgataaat caggtcaatc aggaattgaa aagagtatgg
   105181 atagtgtact tagaaattca aatgaaaagt atataagtgc ttttaaagct ggagatgcag
   105241 gaaatgaaaa atctctaaac atactaaagg gaagtgtaaa aactgttgat aataaggata
   105301 aagatagaca tctaaaaact actattgatt ataacattca aaaaaaatta gaacaaatac
   105361 taaataaaga agaaaatcct acagcagcaa taatctcgga agcatctaca ggtgaaattc
   105421 ttgcaatgtg ctctagacct aattttgacc aaaatgacat atcaaaaagt ttaaaaggaa
   105481 aaaatggaga atttgaaaat agagttataa aagcgacata tccaccaggg tctgtattta
   105541 agatggtagt tttattttct gcacttgaaa atggtgttat agatgagaac tatacttaca
   105601 actgtacagg taagactaaa gttgggaata ctaatgaaat actgcgatgt aataaaagag
   105661 atggtcatgg tttccaaaac ttaagacaag ctttttctaa ttcatgcaat cctgcatttt
   105721 tagatatagc tatgaaactt ggaaaagaaa agatactcaa atctgctgaa aaattacatt
   105781 tatttgaaaa ggttgatatt ggtcttgatg aagaaaaaat tagagaagct ccaaaaaaca
   105841 tatcaataag aaatttagct ataggtcaag aaaatataga atttacacca cttcaaataa
   105901 atcaaatgac tcaaataata gctaataatg gaacattcaa accactatac ttatataaaa
   105961 gtcttgtgga caataatatg aatacaataa aaacttataa gtcttcaaaa aaagaagaac
   106021 ttatttctcc ttatgtgtgt acacaagtta aagaatatat gaagtctgta tctaggattg
   106081 gtacagctaa agacctaaaa gatattgaag gtggttgtgg agttaaaact ggtacagctc
   106141 aaagtagttt aaataaaaaa gctatagacc atggatggat aacaggattt tatcctgaag
   106201 aaagacctaa gtatgtaata actgtgttag tagaaggaac tcaaaaagga aataaatctg
   106261 caacacctat atttaaggaa atatgtgaaa gtataaaata aattatgtac tataaaattt
   106321 aaggatatat cgtaactttg attttagata gaaaattttg tctaatgatg tacgatatat
   106381 ccttttaaaa tatagtatta actttcttga acaagctctt tttctagttt tttaagagct
   106441 tttttttcga ttctagatac atatgacctt gatatatcga gtttttgtgc aatttctctt
   106501 tgtgtcttat agccataagg agttaaccca tatcttaact ggacaatttc tctttctcta
   106561 ggagtaagaa ttttgtttag ttgttcgtaa agttttccaa cttgaacttt tagttcaact
   106621 tcatcaagaa catagttagc ctctgtacca agtatatcta aaagacttat ttcattacct
   106681 tctttatctg ttccgatagg gtcttgtaga gatacttgag ttttattttt tttgttagat
   106741 ctaatattca ttaaaatctc attttcaata catctagaag catatgttgc taatcgagtt
   106801 cctttatcta tgttataagt ttctatagct ttaattaatc ctattgtacc tatggatatt
   106861 aaatcatcct gatcttccgt tgaattatta tatttctttg ctatataagc tactaatctc
   106921 atgtttcttt caattaatgt atcttttgca cttttatcgt tttctatttt aaatttagtt
   106981 aaatactcaa tctcttcttc aggggttaag ggtttttcaa aagatttaag agctgccaat
   107041 aggtcacccc cttttcaacg gtcactttaa attatatgaa ggagacttat ccaagttgac
   107101 tgttatctaa taaaataata tgcatatttt tagaatttaa aacatatatt tgcaaacttt
   107161 tgaatggaat atgacataaa gagtgggaat ttaaatttaa gtttttgttg tatatatatg
   107221 aaacaaggcg aagaaattat tgatttataa agtataaatt ggaattgagg tgtcaataaa
   107281 tataaaagga aatagtattt atatatgttt tggtgttgaa gtaaaagatt aatgcaaagc
   107341 attagagtct ataaaaatat gaatatagga ggtgtaattt tacaatactt aaaaaagtaa
   107401 atgtaattaa ttgtttgtat aaagttgatt acaagcaaaa gtatcagtca tagttaatac
   107461 tgttaaggat gaaggtggtg tgttaattta tatatgtatg ggaagaaatt ttgattacaa
   107521 tgaaaaaact tacaaaagat atttacgaaa aaacttatag ttaaatgttt atatataaaa
   107581 tattatttag gggttgtttt tattattgaa aataagaaag agtggtttta tatcaattga
   107641 atgtataata agtattgcta tattatatgt ggctgtttat ttagtttcta catcattgta
   107701 taattgttat agttttatca gtagaaatat atctgacaga gaaatgttaa gtacagcaaa
   107761 aaaatatata gaagatgaga agtatagaat acaaaatagt aagtatgagt taattgaaga
   107821 taagatagaa aaaaattaca taaatggata tgaaattaac agtagaatag agcaaatttt
   107881 agattattat caatgctatg aaataaatat agagataaaa aatgaattta aaaaactgag
   107941 gtttaatagc tatgttacta gaaaataaag gctctatatt aataataaca ttgataatat
   108001 tttctattat aagtactgtg tgcattatgt gtataggttt aatttataca cataataata
   108061 tgtttgagtt aggatacaaa gacattcaac taaaagaaat ttctttaagt ggaatagaaa
   108121 tatcagtaag taatgtactt tcttgtgttg aggaagctat tgatatatct aataatgaag
   108181 acgaatttag agaatatttc ttaggaaata atatgattga atgtataaaa aaaataaaag
   108241 atacatctta tagtagttta gaagaacttt ctttaaaagt agaaaataat actatttatg
   108301 acagaggaga tttttttgaa tttgatatag aaagtaatgt tagagaaaaa gaatttttaa
   108361 aagcaataaa agtaagagtg aaaatcaaaa atccatggat aggaattgat attgaagatt
   108421 atatggataa atctaataac aataaaattg atgacaaaac attcaataat tataaagcat
   108481 ctgaaaataa tgaaattata aatcaattta aaaatgaaag tgaaaatata aggaatcaag
   108541 aggttgttga atttacagaa gaagatgaag aagaacttaa acaaaacata attgaaaata
   108601 taaagaagaa ttttgatgaa aaaaacctag tacaggtata taagtatagg ggtgtataac
   108661 tttgaaaaaa gaaggaatgt gactgtaata gaattaatag tatctatcag tataatactc
   108721 ttgatagtta cattatcttt tccaaaagat aatcttgaaa atcataaaat aaatttattt
   108781 gcacgtcaac tttgttcaga tataaggtat gttaggcgaa ttaatatgtt tagcaactac
   108841 aatacatata tatattatat aaatcaaggt aaacacgaag gttatgtatt gagaaaagat
   108901 ggtaaaaaca ttaaatcaat aatgctacct aaaaatgcaa aaatagctag gagtattgaa
   108961 ataataaagt ttaaatctga tgggtcgcct caaaaggcgt ctacaataga ggtatataat
   109021 aatagattaa gaaaaactat aacaataaca cctgtaagtg gaagagtact tctgaaagag
   109081 ggaaaatatg aaacataaat atggttactt gcttttggaa agtgtcgtat cattgtcttc
   109141 aatggtgata attatactag ttttatattc tatatttttg tctacaataa acttaaagtt
   109201 aaaagtagaa gataagatag aactacaaca acaaagtctt gaaatcataa aatctatgga
   109261 aggaattatt agcaattcta tgggaataat gaatgtatct aattatgaag aaacatttaa
   109321 aaagacaaca tctataaaat gtagatattt agatgaaaat aataatgaag aaggcataag
   109381 taataaagaa attatattga atgaaagacg aaataagtta tttgtaaatt ctttaaatgg
   109441 cgaaagtagt caagcaggtg gttatgaaat tggagattat gtagatgaaa tgtatgtatc
   109501 aattactaat aatggtcaat acgttaatat taaattgaaa ttatcaaaat gtagtcaaaa
   109561 atatgagact gatttcaaaa taaaagtatg gaattttagt gaaagtatat aaaaatacat
   109621 aaatttaaga gaaagaaatg acatcaataa gtcatataaa atataattat tgagaactat
   109681 ataactaaat aatatcaata tgtaaagtgt atagattaag aatatagagg ataataataa
   109741 aaatatagaa taagtaattt gccaaatact tgtttatatt gtataactta tgatattatg
   109801 atattagtaa aaatttgtaa tcctgacttg acagaattca agtaagagat ataattttaa
   109861 aggcatagta atgtaaaatt tactaaaaaa taagaaattt attttaatat ggaggaacaa
   109921 aaaatggtat cagcaggtga ttttagaaaa ggtgttacat ttgaaaaaga tggacaacca
   109981 tgtttagtag ttgattttca acacgttaag ccaggtaaag gagctgcttt cgttagaact
   110041 aaatacaaaa acttaaaaac aggagctata agagaagaaa gtttcaatcc aagtgaaaaa
   110101 ttccctaaag ctgttataga tacaagacaa atgcaatatc tatacaatga tggtgagtta
   110161 tattatttta tggatcaaga aaattttgaa caaataccat taaactatga gcaagttgaa
   110221 gatgcaatta agttcttaaa agaaaatgaa gttgctacaa taagatttta ccaaggacaa
   110281 ccattccaag tggaagcgcc aaactttgca gagctagaag ttacagatac agagccaggc
   110341 ataaaaggtg atacagcaag taatgtaact aaagcagcta cagttgaaac tggagcagtt
   110401 gttcaagttc cattatttat aaatactgga gataaagtaa agatagatac tagaacaggt
   110461 gaatatttat caagagtata aaaatttatg tatataaaag cttatatgtc ttaataatat
   110521 aagatatatg agcttttagt ttatatataa aaaagaaagc gaggggtgtt acatgaaaca
   110581 tttatatgaa gaagttgcat acttaaaagg cctagcagaa ggactagaaa taagtgctga
   110641 aagtaaagaa gggaaaatga tacataaaat agttgatgca ttagaagtat ttgcagatgc
   110701 tattgtaaca ttggatgaag aacaagaaga acttcaagat tttgttgaat ctatagatga
   110761 agatttagca gatttagaag aagatattgc tgacatggaa gaagatcttt atgaagaaga
   110821 tgacgatgag gatgatgagg acttcagcta tatagaaatg gaatgcccaa attgtggaga
   110881 gttagtagaa atagacgaag atttgttata tgatgatgaa gtagatgttg tttgtcctga
   110941 ttgtaaggct gtgatattat catcagaaga tgattgtgat gatgattgta catgtggtgg
   111001 ttgttcaagt tgtgatgata gagagtaaaa attcataaga attaaaactg cctatgatgc
   111061 ctatgagata ttttgcttca taggtagttt tatttttatg aggaagggta ttatgtattt
   111121 tggttttgca atacttggac aaaaatagta cactagaatt tatagaatag gattttggag
   111181 ggatgtaaat atgaaaataa gtaaaaaatt actagataat atacaaagat ttgaaaatgt
   111241 tataaattat aaatttaaaa ataaagaata tatactagaa gctcttactc atagttcata
   111301 ttctaatgaa aataaaaaat ataattttaa cgaaagattg gaatttttag gggattcggt
   111361 tttaggcata gtgataagtg attatttatt taatgaagaa gctaatctgc ccgaagggga
   111421 actaactaaa cttagagcaa atatagtctg tgaagattca ttaagtgaag tagctaatga
   111481 tataaattta ggtattcaca tgttattagg tagaggagaa gaagcaacag gaggaagaca
   111541 taggacttca atattagcag acgcatttga agctgtaata gctgctatat atcttgatgg
   111601 aggttttgaa agtgcaagac agtttatact tcatcatatg gaaaatataa tttatgattc
   111661 aagaaaagga aatatattta gagactacaa aacgcatctt caagaggtgt tacaagggaa
   111721 tggagaaaat aatatctggt acaggcttat agaagaaaaa ggaccagacc ataataaaag
   111781 atttgtaatg gaagttggaa ttaatgacga tgtacttggt ataggagaag gcaagagtaa
   111841 aaaagaagct gaacagttag ctgctaaaat agctttaaag aaaaaattat gggaaaaata
   111901 aatataatat gtaaaaatat aaaaagaaat atttatatta gaaggatgtt gtagatgaaa
   111961 aagagaatta tacctatatt tgttcctcac aaaggatgtc ctcatgattg catattttgt
   112021 aatcaaaaaa aaataacagg tgtgagtact gatgttacta gtgaagatgc aagaaatata
   112081 attgaggaat gtttagagac tatagataaa gatgcagatg tagaaatagc attttttggt
   112141 ggtagcttta ctgctataga tattgatata caaaaaagtt tattatcagt tgctaaagaa
   112201 tatgtagaaa aaggtcttgt aaaagatatt agaatgtcta caagaccaga ttgtattgat
   112261 gaagatatat taagtatgtt aaaagaatat aagacaagta taattgaact aggagtacaa
   112321 tccttagatg aaaaagtatt attagatagt ataagaggtc atcaatctga aattgtatac
   112381 aagagttcaa agatgataaa aaatagtgga atcaaacttg gattacagat gatgattggt
   112441 cttccagctg acacagaaga aaagtgtata tttacagcaa aaaaatttgt agagctaaaa
   112501 ccagattgtg ttagagtcta tccaactttg gtagtaaaag atacaggtct tgaaaagtta
   112561 atggaacaaa ataaatataa tccatttacc cttgaagaga gtgtaaatat agtaaaaaaa
   112621 gttcttgtac tgttttatgt taataatatc aatgtaataa gagttgggct tcaagctact
   112681 gatgatatac aaattggaaa agctgtttta gcaggaccat atcatcctgc atttagagag
   112741 ttggtagaag cagatatgat aaaagattat ttagaatttg ttattttgca aaataaaaat
   112801 ataaagcaaa tgttggtcaa atctaataaa aagaatattt ctaaaatcat aggaaataaa
   112861 aaaacaaatg ttaaatatat ggaagaaaaa tttggtgtgc ttttaaaaac acaagaatca
   112921 gatttagata taaatcaatt agaaattgtg ttagatggga aaagtttaat tagtgctaat
   112981 atgagagaca ttcatagaaa attgtatgac atttatgacc tttagtttta ataatgaagg
   113041 gagagaaaag atttgtattt aaaaagacta gagttaaagg gatttaaatc ctttccagta
   113101 aaaacagata ttatttttaa agaaggaata acagccatag taggaccaaa tgggagtgga
   113161 aaaagtaata tatctgatgc tgtaagatgg gtattaggag aacagagtat taagagtctt
   113221 agaggtgata aactcgaaga tgtaatattt gcaggtacag atacaaaaaa acctatgaat
   113281 tattgtgaag tggctcttac tattgataat tctgaaaatc aattggaatt agattttaca
   113341 gaagtaacta taagaagaag ggcttataga aatggtgaaa gtgaattttt tctaaataat
   113401 aaaagttgta gattaaaaga tataaaagaa gtctttttag atactggtat tggaaaagat
   113461 ggttattcaa tcatagaaca aggaaaagta gatgaaatat taagtaataa tcctttaagc
   113521 agaagaaaag tttttgatga agcttgtggc atatcaaaat atagatataa aaaacaggaa
   113581 gcagaaagga atttaagtaa tacaaaggaa aatttagaaa gaatagatga tgtatatata
   113641 gaaattgaaa atcaattaaa gcctcttttt aatcaacaga caaaggcaaa aaagtacttg
   113701 gaaataagtg aaaaattaaa gacacttgaa gtaaatagct ttataagaga aattgaagga
   113761 atagaaaaag aattaagtga ggttaatgaa catcgtaagg ttattgaaaa ggagctcaat
   113821 gaaaaagaag aacaaaaaaa tgttgtagag aaaaagcaag aagatattaa taaagaggta
   113881 gaggtattac aagatgtaat tgaaaaatct gtagattata taaattcaat taaaggagtt
   113941 atatcaaaaa aagagtccca aataaattta attaaggaaa gaataagaaa ttttacgaat
   114001 gaaattagta gaaaaaattt agaaataaat gatataaaag agaagctaaa tgaaaataag
   114061 caatacataa aagaattaga aagcaataaa ttatcaggta gtgaagaact atctaccttg
   114121 caggaaaata taaaagtatt ggaagcaagt aaagataaac aaaaaattaa gttagaatcc
   114181 ctaaataatg agatagaatt attgaaagaa agtataattg atatattaaa taaaaaacaa
   114241 gagtttagta ataagctgtc cactttaaac gcaaataaag aaaatatgaa tatcagagat
   114301 gaaaatataa attcagagat aacagagtta aacaaaaata ttgaaataaa atcatctgaa
   114361 ttagatacta taaataaaga atttaatatg caaaatgaaa atttaaagaa tgtaaataat
   114421 agacataaag aattatcaac taatttgcag gattcaatta gtgaacacaa taaactagaa
   114481 gatgaaatac agaaaagtaa atataattta aatggatata attcaaagtt aaatgtctat
   114541 atagatatgg aaaatcatta tgaaggattt aatagaggag taaaagaagt actaaaaaat
   114601 aaaaatttaa aaggagttca tggagcatta ggacagatta taaatgtacc tgaaaaatat
   114661 gagaaatcta tagaagcagc tcttggtgct tatatgcaaa atataataac tgataatgag
   114721 tttagtgcta agtctgcaat aaactatcta aaacagaata atttaggtag agtaacattt
   114781 cttccattaa atataattaa atcaaataaa ataagtctag gaaatttaaa agcaaatact
   114841 aagttcatag gaatagcaag tgatttaatt acatttgatg aaaagtatag aaatataatt
   114901 gaaaatatac ttggaagaac aatacttata aataatatag atgagggcat aaaatttgca
   114961 aaagaaactg gtcataggtt taaaatagta actttagatg gtgaaatctt aaatccagga
   115021 ggttctttga ctgggggaag tctaaaaact aatgggaata tactatccag aaaaagatac
   115081 attaatgaat atacagaaaa gataagtaat atcaagaatg aaatttcaca tctagaatta
   115141 aaaagagaat ctttagacaa agatgtgaaa aatatcaaaa atgaaattga ttcacatgaa
   115201 agtaaaataa aagatttaga aaaaagtata ataataaaaa gtacaagtat aaagaatgtt
   115261 gagtctgaaa ttgaatcgtt aaaaggtagt attactaaat tagaaaatga gaagaatgat
   115321 ttaaattcta acctaaatta tacacttgaa aaaagtgatg atgttagaaa agatatggaa
   115381 gagttagatg atttatacaa taaaaataaa gaaaaaattg atgcattgaa tgaggaaata
   115441 aaaagataca atgatttata tgataaagaa aaatctgaat ttgatgaatt gaacttaagt
   115501 ttagtgaaga aaacagaagt ttacaatagt atagttagag atataaaaag aatatctggt
   115561 gaaaattgtg agctagaaga aaaaaataaa caattagaag agtcattaaa ttatgaagaa
   115621 catgaaataa ttaaattaca agattctata ctcactgaag aaaaagaaaa agaaaattta
   115681 acaaaacaac taggggatag taatagaaat cttgaaacta gaaaaatagc aaaagatgat
   115741 ttaaaaaata gttttgatga aattaataaa gaattaaaaa ctatagatag acagcatata
   115801 gaacttaaag aaagtttatt taaagttggt ggaagattag aaagattaaa aactagtcaa
   115861 gacacatata taaataagtt gtttgagcaa tatgatatga ccttagtaca agcattggaa
   115921 attaaagatg aagatttgga tatagataga aaattcttag aaagtctaaa gagagaaata
   115981 agaagtcttg gaaatataaa tatagactct ataaaggaat atgaggaaat aaaagaaaga
   116041 tacgattttt atagcgaaca aaagcaagat ttagaagagt ctatggaaga gatagaaaaa
   116101 cttatacata ctttagaaga aaatatgaaa tctgaatttg aaattaagtt tgaagaaata
   116161 agtaaaaatt ttaaatatgt atataagaga ttatttggtg gaggttgtgg agaattaact
   116221 attttagata aagaaaattt attagaaagt gatatactaa taacagctca acccccaggt
   116281 aaaaaaatga aaaatttaaa cctattatct ggtggagaga aagctttaac ggcaataagt
   116341 atattatttg ctatattgat tacaaagcca acaccatttt gtatattaga tgagattgaa
   116401 gcgccacttg atgacgcaaa tatatttaga tttggagaat ttttgaagga tttatctaaa
   116461 gagactcaat ttatatcagt aactcatagg agaggaacta tggaagctgc tgattatatc
   116521 tacggagtta caatgcaaga aaaagccatt tcaaaagtta ttagtttaaa attaaaagaa
   116581 gctcaggaaa taacagatat tatataactt agattgacat aggaggaata acagtgttaa
   116641 aaaaattatt tggttttgga aaagacaagg aaaaggaaat agaaaaaaaa gatgctgaag
   116701 aagaaataga agttgaagac agtgttgata atttagaaaa cttggaagaa actatttttt
   116761 caggacttga agaagaagtg atagataaag tggaagatgt tgaagaaaaa aatgaggaaa
   116821 gtgtcaataa tgaaggcact gaagaaatcg aaaactttga aaaaatagaa gttgagatgg
   116881 atagtaatgg aaaagaagta gaaaatattt caaataatga ttctcaagaa gagataatcg
   116941 aagaattaga aaattatgat gaaattgagt ctaaagagat agaagataaa gaagataaga
   117001 aagttaactt gtttgaaaga ctaaaacaag gattgacaaa agcaaagcaa ggaataactg
   117061 atagaataga tgaagtttta aaatcatata caaagataga tgaagaatta cttgaagatt
   117121 tagaggaaat tttaataaca gcagatgttg gtgtaaatac aactatggat ataattgaaa
   117181 gactaagaga taaaataaag caaaaaggta ttactgaacc aataaaggta agagaagaac
   117241 ttaaatctat agttgaagat attcttacaa atgaaaactc tacactagat atagaaccag
   117301 taccatgtat aatattaatg gtgggtgtaa atggggtcgg taagactact acaataggta
   117361 aactagcaaa tagatacaaa aaagatggta aaaaagtttt attagcagca gcagatacat
   117421 ttagagcagc agcaacagag caattagaaa tttgggcaaa tagaaccaat gtagacataa
   117481 taaaacatca agagggagca gaccctggag ctgtagtatt tgatgctata aaagctgcaa
   117541 aagctagaaa aacagatgta ttaatatgtg atacagcagg aagattacac aataaggcta
   117601 atcttatgaa tgaacttgga aaagtattta aaatagtaga tagagaattt ccagaagcaa
   117661 aacgagaagt acttcttgtg gtggatgcta caacaggtca aaatgcagtt gttcaagcaa
   117721 agacatttaa agaagttgca gatataacag gtatagtact tacaaaactt gatggaactg
   117781 caaaaggtgg agtagtcctt gcggtaaaat cagaagtaga tgtacctgta aaacttattg
   117841 gagttggaga aagtgtagaa gacctacaag atttcaatgc aaaatcattt tcagatgcat
   117901 tatttggtaa ctaatgttct tttaaaaata atggttgaca agagtgttgt tatgatataa
   117961 aatacaatag tgtaaagtaa ataaccttta cagtatactg ctaggaagtg taagatatga
   118021 atatagaaaa aatggtcgaa attggattgt tatttgaaca gtataaagaa ttattaactg
   118081 acaagcagaa agaaatagtt gctctatact atgaagaaga ttattcttta ggtgaaatta
   118141 gtgaaaatct gaatgtatca agacaaggtg tttatgatac cttaaagcgt tcagaaaaaa
   118201 tactaagaga ttatgaagaa aagttacact tagtatcaaa gattcaagag caggaaaaaa
   118261 atattaagat tataaaagat aaaattattg acattaaaga agatttattg cacaatagag
   118321 attgtgctaa tttaatccct aagctagaaa atatagaaga tgtatgtagg gagatgataa
   118381 aatgatattt gaaggattgt ctgataaact acaaggggca tttagtaagt taaaatctaa
   118441 aggaaaactt acagaagtag atgtaaaaaa tgctatgaga gaagttaaac ttgctcttct
   118501 tgaggcagat gttaacttta aagtagttaa agatttcata aaaaaagttc aagagagatg
   118561 tgttgggcaa gaagttatgc aaagcttaac tccagctcag catgtaatca aaattgtaaa
   118621 tgaagagctt acaagtttaa tgggagatgt acaaagtaaa gtaatgattt cctcaaagcc
   118681 tcctacaatt ttaatgatgg ttggattgca aggtgcaggt aagactacta cagcaggaaa
   118741 acttggagga tattttaaaa agcaaggtaa aaaacctcta ttagtagctt gtgatattta
   118801 tagaccagca gctataaagc aacttcaagt tgttggagaa aaattggata tacctgtatt
   118861 caatatggga gataaagaaa atccagtaaa tattgcgaaa gcaggtttaa gccatgcgat
   118921 taaaaatgct aatgatttag ttattataga tacagctggt agacttcata tagatgaagt
   118981 acttatggat gagttaaagt ctattaaatc agaagttaag ccacatgaga tacttttggt
   119041 tgtagattct atgacaggtc aagatgctgt aaatgttgca gaaagcttca atgaggcact
   119101 tggtgttgat ggagtagtat taactaaatt ggatggagat actagaggag gtgctgcact
   119161 ttcaataaga gcagttacac aaaagcctat aaaatttatg gggatgggag aaaagctaga
   119221 tgatatagaa cctttccacc cagatagaat ggcatcaaga atattaggta tgggagatgt
   119281 cttaagttta atagaaaaag cacaagaaaa tatagattta gagaaagcta aagaattaga
   119341 gcaaaaaatt aaaaaacaag agcttgattt tgaagatttc ttacaacaaa tggaacaaat
   119401 acaaaatatg ggacctcttg acaagatact tggtatgata cctggtatgg gaaatgtaaa
   119461 agaccaactt ggagatgtag atttgaatgg caaagaaatg aagagaacaa aagccataat
   119521 tcagtcaatg actacatatg agagaagaaa cccaggagta ttaaatgctt caagaaagaa
   119581 aagaatagct aaaggcagtg gaactagtgt acaagatgta aatagactta taaaacaact
   119641 taatgaaatg aaaaaaatga tgaagatgtt tgctggttca caaaagagta tgaagaaaag
   119701 gggcggctta ggcggtttac ctttttttaa ataaaatata aaaaagtaaa aattattcgg
   119761 aggtgactgt aatggcagtt aaaataagat taaaaagaat gggagctaac aaaaaacctt
   119821 tctacagaat agtagtagct gattcaagag ctccaagaga tggaaaattt atagaagaaa
   119881 taggatacta taatccaatt tctgaaccta aacaagtaag aataaatgat gaaaaagcta
   119941 taaaatggtt agctactggt gctcagccaa cagaagtagt taaaaaatta cttgtaaaaa
   120001 acggagtaat agaaaaattc gaagcttcta aacaagcaaa ataatttttt cttggcaatt
   120061 agtttaagta agggctcatt gctaaggtta ctttaggaga tgaaatatat gaaagagcta
   120121 gttgtagata tagctaaggc tctagttgat aatcctgact cagttgttgt tgaggaattt
   120181 gaagataatg atggtatcgt cttaaagctt acagttgctc aagaggatat gggtaaggtt
   120241 attgggaagc agggaagaat agctaaagct ataagaactg tcgtaagatc tgttgcaaat
   120301 agagaaaata taaaagtttc tcttgagata gtataaaata ggaggattag gtataatgcc
   120361 tagtcctttt tatataaatg cgtagtttaa gattttatga tgatatgacc acataagatt
   120421 aaatttttta agaaggtgat aacatgaaag aaaaattaac tcattttaag gtaggtcaaa
   120481 tagtaaatac acaaggttta aaaggtgaag taagagttta tccgcttact gatgatatag
   120541 agaggtttga tgaattaaaa gatttctatc tgggaaaaga tttaaataat aaattggctg
   120601 ttgaaaaagt aagatacaaa ggttctatgg ttataatgaa gataaaaggc atagacagca
   120661 tagagaaagc agaaaaacta aaaaataaat ttatgtatgt atctagagaa gatagcagag
   120721 agcttgaaga aggcgaattt tttatagcag atatgattgg aatggaagtt ttaactgtag
   120781 atggaaaata tgtaggaata ctagaagatg ttcttcaata ctcagcaaat gatgtctatg
   120841 tagtaaaagg tgaggaagat aaagaattta tgataccagc tataaaaaaa tttgtaccta
   120901 caatagatat tgatgaaaga aaaatgatta tagaccctat taagggaatg atagattagg
   120961 tggtaatatg agatttcata taatgacact ttttccagaa atatttaatt cgtacatgga
   121021 tgaaagtata atgaagagag cagttgaaaa aggtataatt gaggttcata tatataatat
   121081 aagggatttt tctaataata aacataaaaa agtagatgat tatccatttg gaggaggagc
   121141 ggggatggta atgactcctc aacctatata tgacacatat aagcatataa taacaactca
   121201 taatataaat aacccaagtg taatttatct tactccaaaa ggtaaggtat acaatcaatc
   121261 tatggcaaag cagatgtctt taaaagaaga tataatacta ctatgtggtc attatgaagg
   121321 aatagatgag agaatcattg atttaatagt tactgatgaa atttcaattg gagattatgt
   121381 attaactggg ggagagctcc cagcacttat aatgatagat tctatatcga gattaatacc
   121441 aggtgtatta aaccaagaag aatcatttga agaagaatca tttaaagaca acttattgga
   121501 atatcctcat tacactagac ctagagattt tgaaggatta aaagtaccag aagtgctttt
   121561 atcagggaat cataagaaga tagatgagtg gagaagagaa gaatctataa ggataaccaa
   121621 agaaagacga tttgatttat ataaaaagtc taatgaaaaa taaaaatata atgaaaaatt
   121681 aatatatatg tatacaataa agtacctata aatggaatta aaaacataaa agtgcttgta
   121741 ataactattg aagtatagta taatataaaa tgttgaaaat acgggcgttc ctctgtttac
   121801 actggtcggg tagttaagaa cgtctatgat atctaggagg aagaaagatg aatgaaatgc
   121861 taagagcaat tgagcaagaa caattaaaaa atgaagttcc taattttgga cctggggaca
   121921 cagttaaagt acacgttaga attatagaag gaaaaagaga aagaatacaa gtattcgaag
   121981 gtgtagtatt aaagagacaa ggtggaggag caagagaaac tttcacagtt agaaaaatgt
   122041 ctttcaatgt tggtgtagaa agaactttcc cagttcactc tccaaaaata gaaaaaatag
   122101 aagttactag aaaaggtaaa gtaagaagag ctaaactaaa ctacttaaga ggtagagtag
   122161 gtaaagctgc taagataaaa gaagctagaa ataaataatt aaaaacttta cttataaatg
   122221 gggggctgat ttatcagtcc tctttctatt ttaatgaaaa ttacaacaaa taattatact
   122281 agaaataaat agaaatattt gttgaaaata tagattattt atctatgaat ttatagcttt
   122341 taaaatgaaa aataaaattg gtaaatattg cattatattt tgtaacgata ttaaaaataa
   122401 gagaacagta ttatgtaaag tatgacgtta aaaagatgac aatattatag tttgtgaaga
   122461 tatatttttt aagtggaatt aggatataat aatataatat taataaggag tgatacatgt
   122521 gtcaaatgaa tacgaagagt atcttatgga tgacaatcta catataaact ggtatccagg
   122581 tcatatgaaa aaaactaagg agttagtaaa aaataactta aaattgattg atgtagtaat
   122641 tgaacttttg gatgctagaa tcccgtttag tagtaaaaat ccagacatag acaggcttgt
   122701 aggagataag ccaagagttg tcgtacttaa taagagtgat atggcagata gggataaatt
   122761 gaatcaatgg attgaatact ataagaaaat taatatcaaa gctataccag tagatacaat
   122821 aaaaggtgtt ggtataaata agataatcga agaatgtaaa aatgtaacta gagaaaaaat
   122881 gagttcttta aaagacaaag ggagaaaaga aagagctata cgaataatga tagttggcgt
   122941 tccaaatgtt ggtaaatcgt ctcttataaa caaattaaca ggtagaaaaa gcactcaaac
   123001 tggagataag ccaggagtaa cgaaagggaa acaatgggtt agattaaagg gaaatcttga
   123061 acttttagat acgcctggaa tactttggcc taagtttgaa gaccaagaag ttgcattaaa
   123121 tttagcattt agtagagcta taaaggatga gattttagat actgaaactt tggctttaag
   123181 acttatagaa aaacttatga aaatagaacc tgagaagttg aaagccagat ataagttaga
   123241 ttgtctagga gaaactccaa ttgaaactat ggatatgatt ggtcataaaa gaggatttat
   123301 aacaggtaaa aaagaactag attatacacg catagccaca actgtattaa atgaatttag
   123361 agatggaaaa ataggaaata taacattgga agttccagaa aacgttaaaa gatagatttt
   123421 aaaacaaaat caaaaattta atgttaaaaa tttaaaaaag ccttttagca aaggcttttt
   123481 tatttttttg aaatatagac aaatatctcc atataaaagt ataaattagg ttagaaagcc
   123541 tttaaaaata ttaatttaag acaaatatac ctaatatttt gaaaattaaa atgagtgtga
   123601 ttattaaaaa ttttagcata ttgacttgct tgtttcgtat aatatataat aagcacaaat
   123661 aaatacaata tattaaaaat ttaatttatt taattttatt aatttaataa taagttctta
   123721 ttgtttaatt caacttactt atgcaagcgt aaacaatcga gacattttat taatttaata
   123781 aggttaaaaa gggggatttt ataatgggta agaccaagga tgttatagta tttggatttg
   123841 cgttatttgc aatgttcttt ggagcaggta acttaatatt tccaccttac ttgggaataa
   123901 ttacaggtcc agaatggtta attgccttct taggatttac gtttgctgat gctggattgg
   123961 cacttctagc agtaatggct acagctaaat tcgatggaaa cgtagttgaa atgtttaaaa
   124021 gatgtggtat gaagttaggg attcttatag gttgtgcaga tattttatgt ataggaccat
   124081 tcttagctat accaagaaca ggtgcaacaa cttatgaaat gggtataatg cctttatttg
   124141 gaacttcaat tccagtgtta ttattctgta tacttttttt tgcgctttca tatgtactaa
   124201 caataagacc ttctaaggtc gtagatatag ttggacagtt tttaactcca gctcttttaa
   124261 tagctttagc atttataatt ataaaaggta ttatatcacc tcttggagat atagtagata
   124321 aacctatgat acctaatgta tttgcagaag gtataggtca aggatatcag acaatggatg
   124381 cttttgcagc aatagcactt gcatcagttc taatagtatc tttaaatgat aaaggatatt
   124441 ctactattag tgacaagtta aaaatgattg gtaaggcggg tgttcttgct tgtggaggat
   124501 tagctctagt ttatggagga ttatgtttcc ttggagcaac tgtatcaaca atgtatggaa
   124561 cagatgcagt acaatcacaa gtaatagtaa atataactga aggattgctt ggaaatgttg
   124621 ggaaagcgat tttagcagta gtagtatcat tagcatgttt aactacatct ataggtctta
   124681 catctgccac aggtcaatat ttctctagac ttacaaaagg taagttgagt tatgaaaaaa
   124741 tagtattagc tgtatctgta ttcagtgcag tagtagcatc ttttggtgtt ggaactataa
   124801 taaagatagc atctcctata ttaagtatag tttatccacc atcaattgtt ttaataattt
   124861 tagctttctt caatgaaaaa attaagaatg ataatgtgta caaaggagca gtatatatgt
   124921 cattgcttgt aagtatacta acagttatat ctagttatgg agtagctgtt ccagtagtaa
   124981 actctttacc tcttaattca ttaggattta actgggtggt gcctgttata atagctggaa
   125041 taattggcaa ttttatacca tcaaagagtc aatctaatac acttggaaca aactaagttt
   125101 tatttaatta aaatataatc aattgtaaat aaatgagtta actaaaagta aaaggagtta
   125161 tttgtacaaa taactccttt tatttttaga ttctatactt aattatttgt atttaatatg
   125221 gaaaaagtat aagaaaatat caaaaaaata tatagtttat tttttaacga taatagagat
   125281 taaaaaagaa ttttagagta attttataaa tttaagtata catattaaat gatttttaca
   125341 aaaatataat ataaataaaa ttgtgacaat aataaaataa taaaacaaaa tttaagacat
   125401 atgattatat aaatgtatta agtaaatggg taaattgttt tcattgagag atatattaag
   125461 aatttttagt taattatgac agataagtat gtaaacgtga atactagcaa ctgcaaagtt
   125521 taagtaagtt ggaagttaga aagtaattga tatgaagaaa aataagttga taaatcataa
   125581 taaggaattt tcagaaaaaa tgattttaag actaaaaaac aagaggcaaa tctacataaa
   125641 tagaataaaa aacgaataaa gttaactgtc aatttagcaa aataaataca aaatactaca
   125701 agaattaacg taaataggtg ctatgacaaa aggaaaacga ttttttaaga atcataattt
   125761 tgaaaattaa aaaaattaaa aatgctattg actgcttttt tttcagatta tataataaac
   125821 acaaaatatt taaaatttaa attgaattaa aaagattata tgaatgtaaa aaaggagagt
   125881 gatatataca gtatataaag caatacactt aataacaaat cgaataatct aaaaaatctt
   125941 aatattttaa ttattcagaa taattaattt tatcaagaaa aattagagaa aaaacaaaaa
   126001 accaactgtg tttaaagtat acaggttgat tttgtttaat taagaaagtt gtattaaata
   126061 ttgtgaagat tttataaatt atgaaacata attgacaaat ttaagtcaaa aatgtataat
   126121 taaataaaat ctcgtcaaaa ggttttcatt taatgaagag attggactta ttttttagta
   126181 aaaggtagta aaattcaaat gggggtgctt taaatgggtg agaaacaaat caattttaaa
   126241 gatgtaattg taattggatt tgcactattt gcaatgtttt ttggtgcagg gaacttaatc
   126301 ttccctccat atctaggagt tttatctgga agtagttggc taatagcttt cataggattc
   126361 ctttttgcag atggtggttt ggcattatta gcagttatag cagcaactaa gttcaatgga
   126421 gacacatcga agatgttctc aagagcaggc aaaggattaa gtattgcatt aggatgtgca
   126481 atggtaatct gtatagggcc acttttagca atacctagaa cagcagcaac aacttatgaa
   126541 atgggtatat tacctacaat aggttctgga ataagcccag taatattctc tatagtattc
   126601 tttgctctag tattggtttt aacaataaga ccatcaaaag ttgtagatat agttgggtca
   126661 atattaactc cagctttact tatagcttta gcagtattaa tagttaaagg tatcatatct
   126721 ccattaggag aaataagaga cagttcttta atacaaaatg tatttgcaga aggtataact
   126781 caaggttatc aaacaatgga tgcattggca gctagtgtat ttgcatcaat tataatcatg
   126841 tcagtaatag ctaaaggtta tacaggtgag aaagaaaaga tgaaagccac aataagtgca
   126901 ggtgtaattg cagtaatagg aatggcatta gtttatggtg gattatgtta tcttggagca
   126961 actgtgtctc aacaatatgg acaagatgtt caacaaacag ctttaatagt tgcaataaca
   127021 gcagctttac ttggaactac aggtaaaatt ttattggcta ttatagttgc attagcatgt
   127081 ttaacaacag ctataggtct ttcttcagca gctggtcaat atttctcaac acttacagat
   127141 ggaaaattaa agtatgagca tatagttata gtagtttgtg tatttagtgc aatcatatca
   127201 aactttggag taagtacaat aatacaattc tcttctccta tactaagtat ggtatatcca
   127261 gcaactataa ctctagttat actagcatta tttagtaata aaattaaaaa tgacaacgtg
   127321 ttcaaatgtg ctgcatacat ggcattatta gtaagtgtat taacagttgc aacaagtttt
   127381 ggtgtaaaaa ttcctctagt taatagctta ccatttgctt cattaggatt taactggata
   127441 gttccagttg taatagcagg aataatagga aactttattc catctaaaag cagtaaaact
   127501 gcataataat aaaaactaat tgataattat ggaattgcct ttaaattata taggcaattc
   127561 ctttttatat ttaaaagaat tattaaatta ttgtagataa aagaacatta ttaatcct
//
LOCUS       DAEPIN010000003       108650 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.003, whole genome shotgun sequence.
ACCESSION   DAEPIN010000003 DAEPIN010000000
VERSION     DAEPIN010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 108650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 108650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..108650
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.003"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            complement(11..790)
                     /locus_tag="KR250_000243"
     CDS             complement(11..790)
                     /locus_tag="KR250_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0066215.1"
                     /translation="MKVNENNINNIDEELDELSRKVYLESPDFWISISDKIIDKVFDE
                     MLLFFAVKYNYFNIVCFAIENNYLDLNMPSKNKKYTNIMEHLLDTSKQNEDKKIYNYL
                     LSLNKGNNLDERPILDELNCNISSDTFEKKDIYLPKYLCPSCNSNIFDTGYKVSNDIT
                     YKFSSKEAEPIEMCSEVSSIRCCNCDELLEDITLDKLYNLCKIQNCNNCGSNLTKVGI
                     IAKKKMNFDENTEKFKDVSTSYCCGNCDSEINLSQREHFKI"
     gene            1114..1659
                     /locus_tag="KR250_000244"
     CDS             1114..1659
                     /locus_tag="KR250_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HBH0066216.1"
                     /translation="MKNTKVNIQDLTKMSICVALLCISSYIYIPLPFTPAGVTAQTIM
                     INLIALILTPRQAFSTVGVYIMIGLIGIPVFGGGTSGIGKLLSPTGGFYFGFLFAALI
                     ISLLKGRNNDIKRYILITIFVGMPIIYFMGTVFMCYFNQMTVEAALFAAVVPFLIGDT
                     IKSVIASFLGTKLNNVLRRNL"
     gene            1691..2827
                     /locus_tag="KR250_000245"
     CDS             1691..2827
                     /locus_tag="KR250_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HBH0066217.1"
                     /translation="MKEVFILGGLRSHIGLKNGIFQFVQPELLGASVLKDLIKKYEID
                     KIDEIICGNAVGTGGNIARLMTLTAGVSNEVPAFTVDMQCASAMMSIDIAFSKVKSGQ
                     CDLIIAGGFESSSLQPMRTYHKNDKRYNTNNPNYTVAQFSPDDNSQNSMLEGAERVAE
                     LYQIEKADLDFWVKESHKRAKEAREEKILEDIISPINNSTYDEGIRDKMSQRLLDRMP
                     SILGKETITNAANSCLINDGASFIIICSKKYLEHVKKKPKAKIINTCTIGTDATLSPT
                     SAIQAMDKLLEIESLNYLDVSAVEFNEAFAVIDVLFQRKYPELIDRYNIFGGALAYGH
                     PYGASGAIIALHLLKALEKTKGRYGICSIAAAGGLGSALLLERV"
     gene            2831..4222
                     /locus_tag="KR250_000246"
     CDS             2831..4222
                     /locus_tag="KR250_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HBH0066218.1"
                     /translation="MNYYELLRIKKERHKNKNFLIIDGEKYTYENILSYSEELGKQIS
                     LGENIPILIYSKNIMFQLISFFAINYSKNIPIICHYNLSKKVLDDILLKNNISIIISD
                     ESMDVIDLYDDNNNEVAKKVNFSIFNPKFNIYMYQYKNLINYLDKSICMGALSSGSTS
                     VPKVLYRTYESWAGFFPIQNGIFNISGESILFVNGTLSFTGNLNSIMSVLYEGGSIVI
                     SSGLNCKSWIRIIKQYNVTNIYLIPTKLQLLVKHLKEPVLKVVSIFTGSQLLFEDTAR
                     NLKKYMPKSEIILYYGASELNYITYLCYDELIEKPLSVGRPFPGIDIYIREGKIFVNT
                     EYAVYNATKPYSVNDIGYYDNDGYLIFEGRSDDILNIGGFKVSSTKIENEVKKIPQIE
                     DAIVLPYSDTIRGNQIVLFVITIDKITKKDLLIKMKDNLMKNEIPKKIIFLNSFPYTS
                     SEKIDRLALLKML"
     gene            4298..5173
                     /gene="dpsA"
                     /locus_tag="KR250_000247"
     CDS             4298..5173
                     /gene="dpsA"
                     /locus_tag="KR250_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit DpsA"
                     /protein_id="HBH0066219.1"
                     /translation="MSNQYDITVIGGDLRQVYMVKKLINKGYSVVTYGISNEIINGIV
                     GKSTSLAEALSKSSIILCPIPFTKNQVNIENTDDCLDATIDNLLINLSPNQILFGGNI
                     PTKVCKFCEQHSIQIYDFMKMNDVAILNAVATAEGAIAEAIKRSIINIHQSNCLVLGY
                     GRCAQILAKKLYALDANVCVGARNNDARSTANAFGYSSISLSQLDEKLLQFDYIFNTI
                     PECILTKERLEKVSQEVTIIDIASSPGGVDYSVVKEAGINATLCLGLPGKYSPKTSGE
                     ILVNAIENIINERRD"
     gene            5176..5766
                     /locus_tag="KR250_000248"
     CDS             5176..5766
                     /locus_tag="KR250_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit B"
                     /protein_id="HBH0066220.1"
                     /translation="MRLEGLTIGVGFTGSFCTYDKIFIELENLVKEGANVHTIFSDVS
                     QNIDCRFGNSEEFMKKAYELTGNKPIVTIEEAEPFGPKGIADIIIIAPCTGNTAAKLA
                     NGITDSPVLMAAKGHLRNDKPLVISISTNDALSFNFKNIGILLNSKNIYFVPFGQDNC
                     KAKPNSMIAHTELIIPTIELALENKQLQPVIKSPHQ"
     gene            complement(5973..6173)
                     /locus_tag="KR250_000249"
     CDS             complement(5973..6173)
                     /locus_tag="KR250_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0066221.1"
                     /translation="MEKLKTISVFSLIISVILTIGGIGIVTYYVDNLFIRGLSVFVLI
                     MSSSFVSTTVRLIFEESKRYKF"
     gene            6677..9331
                     /gene="adhE"
                     /locus_tag="KR250_000250"
                     /gene_synonym="adhC"
     CDS             6677..9331
                     /gene="adhE"
                     /locus_tag="KR250_000250"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HBH0066222.1"
                     /translation="MAHKEQVIIENKKENEVMSVGLDTVNNVESLVKKLAKIREAQKI
                     FATYSQEQVDKIFLAASLAANKQRVPLAIMAQKETGMGIAEDKVIKNHYASEYIYNAY
                     KETKTCGVIEKDEAFGMTKIAEPIGVIAAVVPTTNPTSTAIFKALLALKTRNGIIFSP
                     HPRAKNSTIEAAKVVLEAAVLAGAPEGIIGWIDEPSLELTTTVMKEVDLTLATGGPGM
                     VKSAYSSGKPAIGVGAGNTPAIIDDSADIKTAVNSILVSKTFDNGMICASEQSVIVLE
                     NIYNEVKKEFKERGAYLLDKDETEKIRNIILVNGGLNSKIVGQTACTIAKLAGFEVPV
                     DTKVLIGEVESVEIEEAFAHEKLSPVLAMYKASDFDDAVRKAEKLVEDGGFGHTSSLY
                     IDDVNQREKLDKFTSAMKTCRILINTPSSQGGIGDLYNFKLAPSLTLGCGSWGGNSVS
                     ENVGVKHLLNIKTVAERRENMLWFRAPEKVYFKKGCLGVALKELKDVMNKKRAFIVTD
                     TFLYNNGYTKAVTDLLDEMNIKYTTFFEVEPDPTLECAKIGAKAMREFNPDVIISIGG
                     GSAMDAGKIMWTLYEHPDVDFQDLAMRFMDIRKRVYTFPKMGEKANFVAIPTSAGTGS
                     EVTPFAVITDQDTGVKYPLADYELMPNMAIVDSDMMMNMPKSLTSASGIDALTHALEA
                     YVSMLATDYTNGLALQAIKSIFEYLPRAYDNGAKDPEAREKMANASTMAGMAFANAFL
                     GVCHSMAHKLGAFHHVPHGVANALLITEVMKFNSSDAPKKMGAFSQYKYPEALKRYAE
                     IASFLGLKGNSDEEKFQSLLVAIEDLKLKVGIPKSIKEFGVEESKFMDSIDEMVIQAF
                     DDQCTGANPRYPLMNEIKDMYLNSYYGR"
     gene            9607..11826
                     /locus_tag="KR250_000251"
     CDS             9607..11826
                     /locus_tag="KR250_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBH0066223.1"
                     /translation="MTGRNKSFTLITILFLTSLFLTMGYIYIEDTKHLLMSEAKIHIK
                     EVAVQGSQLAQRQIEKDLDVLNIFSNYYASNPDIPNEEKMKNLLDEMENQKFYTMAIV
                     DINGNAENTKGDKFSVKDREFFKNSIKGKKYVSSPYVDEVNKSIKKIAISVPLLNNDK
                     VVGVLYCTYNINTLMKLINISFYENNSISYVVKNDGTIILHPQGDSLSKNIYKLLEQD
                     NDIQEVNRLKKELQENKTGATVLNMLEERRYLGYATMDNGNSENNYIKDWNLIFSIPE
                     TVVFSNSKQIINRAVYAVLSIVLIFVAIIFYIIYIKKSNEKEILSLAYEDKVTYIGNQ
                     NKFYRECSKYLLDKPSLNYIIVYFDINNFKMINDTFGYEFGDNLLITIAKALKEELTE
                     GEVYARLSSDYFAIFCDYKNGRNKIIRKLDNIRSNIESNLSIVFEISLCVGIYFVEEG
                     EVDIQKAVNKANMARSVAKGKNINYAIYNEDVRNKLSEESMILDDIKIALVKNQFEVY
                     YQPKFSLVTGEMIGSEALIRWNHPEHGFISPAVFIPIAEKSKLILKIGRFVFERVCND
                     LYEWKKQGKKIVPVSVNLSRVELYQPDIVKFINKTIKMYNLSSDFIEIEITETVAINE
                     LNILKNVLNELRTYGFSISMDDFGTGYSSISCLRDMPIDILKLDKSFLGGIEHDERSR
                     NIAKSIVSLAKSLDLVVIIEGVESKEQAELMKQFGCDLVQGFYFARPMPAKNFLDLL"
     gene            12080..12742
                     /locus_tag="KR250_000252"
     CDS             12080..12742
                     /locus_tag="KR250_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HBH0066224.1"
                     /translation="MIDISIDDKLKERWPNAKLGCIQAKVAVNKSSEKLINEINEFCD
                     TINQSLKVEDITKLDKIKDARNAYKELGKNPSRYRTSSEALVRRIIQGKGLYTINNIV
                     EINNLISIKSLYPVGTYNVSKLHSPVCFTVGDEGEQYKGIGKELINIENLPILSDSVG
                     KFGSPTSDSERAMITEDANEILICIFSFSQESDIEEYFGYAKELLKEYADGKDIETKI
                     IK"
     gene            13113..14522
                     /locus_tag="KR250_000253"
     CDS             13113..14522
                     /locus_tag="KR250_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HBH0066225.1"
                     /translation="MIDGKEVKQESGQKNLKFKIAIVVGVLFVAYIGMAIFFRNHFYF
                     GTTINGVKASGKTVEQVNDLLASSTNSYTLNLEERNNKKEQIKAKDIDLKFTPDDKVQ
                     KIKDSQSAIGWIGGIFKSKEYNNMITLSYNKDLLEKRFNALSCFDSKNVVEPKSAYPK
                     YDSTKNAYVIVPEVLGNKVKKDDFYKLVQNSINSGNTSISLDESKVYEAPNNTKDSEK
                     LKQAIKTLDKYVGVVVTYDFSDRKETLDGSEINKWLKVDSEKNYDITFDNDAMVEYTR
                     GLSKKYSTFGDSRPFKTATGKDITISGGIYGWLIDKKKEAEALVDVVKAGKNVTREPI
                     YIQKAVSRNKDDIGNTYVEISLSGQHMWFFKNGEMLVSTDVVTGDLARGFATPSGIYP
                     LNYKARNVSLTGQGYSSPVSYWLPFNGNIGIHDATWRNSFGGSIYKSSGSHGCVNTPF
                     SKAKKIYENIEPGTPIILY"
     gene            14637..14912
                     /locus_tag="KR250_000254"
     CDS             14637..14912
                     /locus_tag="KR250_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif11-like leader peptide family natural product
                     precursor"
                     /protein_id="HBH0066226.1"
                     /translation="MNEKLTKILEEAKNDDEFRKELIKTREQRDVMDSFCKVVTSRGY
                     DMTVGELFSMGEEYTSNLLKSVNGGAAYPIEDWSDWYEDFFIMLSNI"
     gene            15542..15853
                     /locus_tag="KR250_000255"
     CDS             15542..15853
                     /locus_tag="KR250_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0066227.1"
                     /translation="MAFEIIKSIVEAEQTADSIKVKAVTDAESIRADAVNKCESIFAD
                     VKKQAKLMEETLIEKAVTDSRAEVDKILANAKSECLKIEKTAEERKSKAIEAVIGKVV
                     R"
     gene            15855..17780
                     /locus_tag="KR250_000256"
     CDS             15855..17780
                     /locus_tag="KR250_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HBH0066228.1"
                     /translation="MAIVNMNKISIVGLEAQKSQILKLLMNRGFVQIDDSAFLTEEEE
                     LKNYLVKDSEESEVIMLEQKMSHVNQAINIVSSLVKQKKSLLAPKREFNKIDSEKEGQ
                     VYAEALELNNLHKEIGTIRSNINLLNNNKSMLEPWVNFDIPIENMETKYTKTILGTVP
                     ISANIDALRVKLEDEIPESVIGIVNSDKLMNYVYLITLKETFDDVLEVLKEFNFSVVS
                     LPNEEGTPLQAIKKYNEMIIRQENVSKDKTEEIKRHADSIHMLENLYDFYTIERDQKK
                     IIERLVKTKTTFCLNGWLPSKRADELIKEITEKFDCCVQTEEGSKEEGFPILLENNKL
                     VTPFESVTNMYSYPSTKDIDPNTILTFFFVIFFGMMLSDAAYGLIIAVVCGFVVYKLK
                     IQKGEGNLIKLIGICGVSTTVWGLIFGGILGDLIPIKALINPLEDVMTLMGMSLLFGI
                     IHIYVGLGIKAYTLIRSGKVIDAIFDIGFWYLCITGVCLLIIPFVAGDIGVFSEVGKY
                     LAIIGAIGLVLTQGRSFKGIPVKLFKGFSSLYGITSYFADILSYTRIMALCLTTGVIA
                     QVINLLGAIAGPILAVVIGVVGHTINLLINALGAYVHTSRLQYVEFFNKFYEGGGVPF
                     VPFKYKTKYTSINKKEM"
     gene            17783..18274
                     /locus_tag="KR250_000257"
     CDS             17783..18274
                     /locus_tag="KR250_000257"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HBH0066229.1"
                     /translation="MEFLNALGNGQFLAISGAAIAALFAGMGSAKGTSIAGQAAAGVV
                     TEDPSKFGQLLVLQLLPGTQGLYGFIIAFLILSKAGIIGGDAIPTSAQGLQLLMAGLP
                     IGLVGLVSGIAQGKAAAAGVGIVAKRPEELGKAITFAAIVETYALLGLLVSFLAYNGI
                     AIG"
     gene            18326..18889
                     /locus_tag="KR250_000258"
     CDS             18326..18889
                     /locus_tag="KR250_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HBH0066230.1"
                     /translation="MGNEQKMIDRIIADAKQEAQEILDKAKSEADLKVNSANEKAEKE
                     MASYTKLAEAEAEKAASKEISGAYMEAKKQILSKKQEILEEVILEAKNKLLNLKDNEY
                     EEIILNMIEKSNCTDDSEIVLSKKDKKTLKDVLSKKGIKVSDETRDITGGFIVKKGDI
                     EYNYSFEAIIAVEHEYIEQIAAEILFN"
     gene            18904..19881
                     /locus_tag="KR250_000259"
     CDS             18904..19881
                     /locus_tag="KR250_000259"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit C"
                     /protein_id="HBH0066231.1"
                     /translation="MAEEKTYPYAVARIRVLEKQLLNRQMFIQMAEAKSPEDSLRIIA
                     EAGYADTSNNNIHNFENILTQELSKTYEILKSLAPEEKFVDVFLYKNDYHNLKVLIKE
                     EISGVDGEKYLIDGGTIPLIKLRESLANRSFSDLPKIMSSAILEAFDVYNKTQNGQMV
                     DIVLDKATFTSMKETAKESQNKFVIDYVMKVCDLTNLKSFIRVKNMKKDFDMFMNVFV
                     SGGSLDKEKFLEAFSSDTPASCFKSTSYSDVCKNGMDSGFTVFEKLCDDYLMEYVRGA
                     KFKPLTLEPLIAYLYAKESEIKTIRIILTSKLNNIDADTIKERLRDAYV"
     gene            19868..20191
                     /locus_tag="KR250_000260"
     CDS             19868..20191
                     /locus_tag="KR250_000260"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HBH0066232.1"
                     /translation="MLMYKVGVVGDKDSIMGFLALGIDIFPAYDSDEIKKSIHKLVED
                     EYAIIYITEQASLLAKESIAKYKDYQLPAIIVIPGIGGSMGLGMNEVRESSKRAIGAD
                     ILFKE"
     gene            20308..22086
                     /locus_tag="KR250_000261"
     CDS             20308..22086
                     /locus_tag="KR250_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HBH0066233.1"
                     /translation="MKTGTIVKVSGPLVVAEGMRDANMFDVVRVSDKHLIGEIIEMHG
                     DKASIQVYEETSGLGPGEEVVSLGMPMSVELGPGLISTIYDGIQRPLEKMYDISGTNI
                     TKGVEVSSLDRTTKWEFKPKKSVGDKVVAGDIIGGVQETAVVECKIMVPYGVKGTIKE
                     IYSGEFTVEDTVCVITDEKGNDIPVTMMQKWPVRKERPYREKKTPDSLLVTGQRVIDT
                     FFPITKGGVAAIPGPFGSGKTVTQHQLAKWADADIVVYIGCGERGNEMTDVLLEFPEL
                     KDPRTGESLMQRTVLIANTSDMPVAAREASIYTGITIAEYFRDMGYSVALMADSTSRW
                     AEALREMSGRLEEMPGEEGYPAYLGSRLAQFYERAGKVNCLGMDEREGTLTAIGAVSP
                     PGGDTSEPVSQATLRIVKVFWGLDASLAYKRHFPAINWLTSYSLYQEKVDVWADKNVN
                     DNFSAQRAQAMKLLQVESELQEIVQLVGVDSLSDGDRLILEVTRSIREDFLHQNSFHE
                     VDTYTSLHKQYRMMGLILKFYKSAQDAIKQNVTINDIIKLSVLEKIGRAKYVEEGDID
                     AAYDSIEDEIDNELADLIKKRGAEEL"
     gene            22083..23456
                     /locus_tag="KR250_000262"
     CDS             22083..23456
                     /locus_tag="KR250_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HBH0066234.1"
                     /translation="MIKEYKSIQEVAGPLMLINGVEGVKFDELGEIELSNGEIRRCKV
                     LEVNGDTALVQLFESSTGINLAESKVRFLGKSLDFGVSPDILGRVFSGMGRPIDGGPE
                     IIPDKRLDINGAPINPAARDYPAEFIQTGVSAIDGLNTLVRGQKLPIFSGSGLPHANL
                     AVQIARQAKVRGTDSKFAVVFAAVGITFEDAEFFISDFKATGAIDRSVVFVNLANDPA
                     VERVSTPRMALTAAEYLAFEQDMHVLVIITDITNYAEALREVSAAKKEVPGRRGYPGY
                     LYTDLATMYERAGKMKGHEGSITMIPILTMPEDDKTHPIPDLTGYITEGQIILSRELY
                     KKDIQPPIDVLPSLSRLKDKGIGKGKTREDHADTMNQLFAAYSRGKDAKELMAILGES
                     ALSEIDLMYAKFADEFEKEYVSQGFRTDRTIEQTLEIGWKLLSMLPKSELKRIRDEYF
                     EKYMPKE"
     gene            23462..24130
                     /locus_tag="KR250_000263"
     CDS             23462..24130
                     /locus_tag="KR250_000263"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HBH0066235.1"
                     /translation="MARLNINPTRMEMTRLKKLLKTATRGHKLLKDKLDELMKQFLEI
                     VRENKRLREEAENALDTAYKNFIIARAVMSQEYLGSALMMPKQSVSVDVSTRNIMSVD
                     VPVFDFKTENNQSDIYPYGLAFTSGELDSAMEAFSDAMQPLLRLAESEKSAQLLAQEI
                     EKTRRRVNALENVMIPNYIETIKYIAMKLEENERASTTRLMKVKDMVLKKALEEKKKN
                     DLVV"
     gene            24407..25456
                     /locus_tag="KR250_000264"
     CDS             24407..25456
                     /locus_tag="KR250_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HBH0066236.1"
                     /translation="MKEFIFTCLCIVVITFMTSCISTTPQTTQGKGNNKLTIYAALEE
                     EQIAYYMEGFREEYPNIDVEIVMDSHGVIASKLLAEKDNPQADIIWGLSAMNMIELKK
                     DGALEAYKPKNYDKVDKKFKDTEDDVSWVGMSVPETAIVVNYKELKKLGIDIPKSYED
                     LIKPEYKGLITMPNPASSGTGYLTVSGLIQMMGEKDAYNYMDKLHKNIATYTHSGSKP
                     AKLAGSGEYPIGITLGYRATQQLSSGEPIEVVFPKEGSGWDLEANALVKKDNIKKEAK
                     LFLDWAISDSAMDRYAHEVAVTSIKTDNPVPKGYSKQPFEQLIKEVDLNSAAKNRSTV
                     LKTWESKYGSKSESK"
     gene            25552..26541
                     /locus_tag="KR250_000265"
     CDS             25552..26541
                     /locus_tag="KR250_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBH0066237.1"
                     /translation="MSYLKINNVFKSYDQKKVLNNISLDIEEGEFLCLLGPSGCGKTT
                     LLRIIAGLEDVNSGTIILQDKDITNLEPSKRGFGIVFQSYALFPNMTAYNNIAFPLKE
                     RKVSKEKIDNKVKEVLETVGLTNEAHKYPKALSGGQQQRIAIARALALEPKFLLLDEP
                     MSALDAKVRHKLRMDIKRLQKELNITTIMVTHDQEEAITMADKIAILNGGDIMQIGTP
                     EEIYQNPQNLFTAQFIGDTNCFDNGDSILTVRPEYVQIEKSTKENYQGIISNIEFRGN
                     LLRVEIKDKLNENFIISDVSIKEWVNLNLVEGDLVKISIDEKYYLKYPKVKGA"
     gene            26546..28264
                     /locus_tag="KR250_000266"
     CDS             26546..28264
                     /locus_tag="KR250_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     permease subunit"
                     /protein_id="HBH0066238.1"
                     /translation="MNNNAISSKSISLKSKMNLDIKKCMLTVLVMSLVIFLLAPIIYL
                     FVQAFLDKDGVFVGLQNFQEYLASESLLNSLKNTVFVSVISTVISLTLALVYAYALTR
                     TKIKFKGFFKSLAMFPMFAPTMMHAIGLVYLFGNQGLVTNLLGVHVPIYGAVGIILAQ
                     IIYVFPQSFQILYISLSSTDYRLYEASNTMGISKIREFISITIPSIKYSLISSAFVSF
                     TLCFTDFGAPKVIGGEFNVLSIDVYKQIIGQQNMPMGATVSILLLIPAIISFIVTKKI
                     EKNQNSYITSKSIPYKVKTNKLRDSILGGVVGIIAFGIIAVMLTVILSSLISVWPYDL
                     RLTLEHFSMKNSIDGIKPFINSIKMSLFTAILGTSFVFLFAYLNEKTEKAPYLKSFGY
                     FLSTLPMALPGLVLGIAYVLFFNKLEFNFMDTIYIKNLFNGLYGTLLIMVICNIVHFF
                     SVAFITATTAIKKVDKELDMVAKSMGINSASILKNVTIPLCLPAVLENFMYFFLNSMV
                     TISALVFIYTASSKVAAISIVQLDDKGSTAQAAALAVLILVTNILVKVIYEFIKNRLE
                     KRTNFN"
     gene            complement(28346..29500)
                     /locus_tag="KR250_000267"
     CDS             complement(28346..29500)
                     /locus_tag="KR250_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBH0066239.1"
                     /translation="MKINKKFNRVDMQVSILTAIIVIISSSCVYFFNYTLTYKSMIYS
                     LSERSKHIYEYVERNIDKNTFTEINSKEDQSKKSYKSSKILLESVKNTTGVMYLYTAK
                     KTKDGSLVYVVDGLNSSRSDFRNAGDLIEKEIWGDLNKALDNHILLPKKIKSTSWGYI
                     FISYFPIHNDVGKVVGVIGIEFEAKHQYNTFKFISIVTPIIALLTCLISALIAVILFK
                     RISNPTYTDFANTDYLTGLKNRNAFEIDMNNLNNSNLKNLISIISIDLDGLKKINDKF
                     GHQTGDLYIKQASKIIQYVINKKYIVYRIGGDEYIIILKNLSHKEINNIINNINLKIV
                     EENSTNELKLSMSIGYAIFDEKVDVSLFDTYRRADSQMYDNKRKINKQYQ"
     gene            29999..31360
                     /locus_tag="KR250_000268"
     CDS             29999..31360
                     /locus_tag="KR250_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBH0066240.1"
                     /translation="MKDRLNLTEGRITEKLLKLSLPIMGTSFIQMGYNMIDMIWVGKA
                     GSKAVAAVGTAGFFPWLAMAFIMISKVGGEIKVAQSIGANNISTTKSYMKSAIEINII
                     LAIIYTISLIVLNKQLIGFFRLGDLEVITMSRQYLIIVALGMVFYFINPVFTAIFNGL
                     GNSKTPFRINTVGLITNIILDPILIFGWGFAPKLGVAGAAIATVFAQIVVTTCFIYII
                     VKSKEEYFKLRLFKEIETKYYKVLYKLGLPIAIQSGMFTVFSMLLGVIVASWGPVAVA
                     VQKVGSQIEAISWMTAEGLSVALGSFVGQNYGAKKYSRINKGCKIAITVSSVLGIITT
                     LVLVFAGESIFSIFIDESEAIEKGAVYLKILGYSQFFMCLEIITTGAFKGLGRTYIPS
                     IIITILTGARVPLAYFISRPEMLGLNGVWWSITLSSILKGILMISLFISLLKLGKLYK
                     ISE"
     gene            31451..32155
                     /locus_tag="KR250_000269"
     CDS             31451..32155
                     /locus_tag="KR250_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBH0066241.1"
                     /translation="MKEPIYKVIENHVRELINSDSLKEGDLIPSEKQLSEEFNVTRMT
                     VRSALNNLVKEGYITRQRGVGSIVIANNIYDNISSVSGFTKEMESKGYKVSNILVSLE
                     IVQADENLSSKLNISLEENVWEIKRVRLANDARVSYMITYMPVKLFPNLNKTHCENSL
                     YNFVEEVCEYKIAMSEREVQAVISNEECMDNLKLEEPEPLLYISQICKLQNSEIFEYS
                     HTYHYGYTLTLNAVVE"
     gene            32355..32666
                     /locus_tag="KR250_000270"
     CDS             32355..32666
                     /locus_tag="KR250_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBH0066242.1"
                     /translation="MIKIMLACSAGMSTSLLVTKMEDAAKENGIEAKIWAISEVNLKN
                     EIENCDVLLLGPQVRYVLGKATEMAKPYNIPVEVINMMDYGRCNGKAVLDRAVELNSS
                     K"
     gene            32688..34001
                     /locus_tag="KR250_000271"
     CDS             32688..34001
                     /locus_tag="KR250_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBH0066243.1"
                     /translation="MEKFMSFMDKYIVPVAAKIGAQRHLVAVRDAFIVMIPITMVGAL
                     GTLINNLPLEAYKNLMASIFGENWTTFGGDLWWGAIGTMAVFLVIGVAYFLAKSYESD
                     GLQSGLIALSIFFIMAPQIGKIVPEGGTTVVEGWGMIQQTYLGTAALFSSILIGLLST
                     EIFVRLSKVKKLTIKMPDGVPPAVSRSFAKLIPGMLTIMIFTVIGIFIKMLSNGSFLT
                     DILNTYLGAPLSNVADSLGSTMLIAFIIHILWTVGLHGANIALPFTETILMKLGGENA
                     ALAQAGATEGYHVLAGAFFDAFVYLGGSGMVLGLIVALLIAGRRRKEMIVLGGPPAIF
                     NIGEPLIFGLPIVLNPIFMIPFVLAPVICSAVSYLAIDFGLVAPVILPKIPWVTPPIL
                     GGAMATGDWTGGALALFNLILSILIYIPFVIASEKMEANKLKINN"
     gene            34098..35408
                     /locus_tag="KR250_000272"
     CDS             34098..35408
                     /locus_tag="KR250_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBH0066244.1"
                     /translation="MKKGLKIVTIGGGSSYTPELVEGFINRYEELPVKELWLVDIEAG
                     KEKLEIVGNLAKRMVKKAGVDMKINLTLDRREALRDADFVTTQLRVGLLDARIKDETI
                     PLSHGVMGQETNGAGGLFKALRTIPVIFDIIKDVEELCPNAWMVNFTNPTGIITEAVF
                     KYTNFRRYIGLCNVPIHLKNDVAKLFNVESDRISMDFAGLNHMVYGLNVSLDGEDVTK
                     EAIDKFVKADISMQNIKAIDFNAEFVKSLGAIPCPYHRYYYKTKEMLEDELREFKEGK
                     ARGQVVKELEEQLFELYKDEKLDVKPPQLEKRGGAYYSDAACNLISSIYNDKKDIQVV
                     NTLNKGSIRDFKDEQAVEVSSVITKNGPKPISIGYLPDSVHGLVSQIKSFEVLAAKAA
                     VYGDYESALLALCINPLIPSDDLAKTILDEMLEAHKDYLPRFNR"
     gene            35434..36165
                     /gene="chbG"
                     /locus_tag="KR250_000273"
     CDS             35434..36165
                     /gene="chbG"
                     /locus_tag="KR250_000273"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HBH0066245.1"
                     /translation="MTKIIINADDFGYCEAVNYGIISAHNNGIVRSTSMMANMPGVEH
                     GVGLLKENKTLNCGVHMTLSCGRPLLSNLKTIVDKDGFFIRRITDEIIEKMDCDEIYR
                     ELCAQIDRVKGLGIDISHLDSHHHIHTLVSLKPVIEKIVTKYNLPIRGGFEYNLEYSK
                     VVPLIDSFYKENVSEEYFIKNIDEIMKYDVVDIMSHPAFLDDYILNSTSYAIDRTKEH
                     KILTSKKVKEFLEKNGLVISSYRDI"
     gene            36178..36495
                     /locus_tag="KR250_000274"
     CDS             36178..36495
                     /locus_tag="KR250_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBH0066246.1"
                     /translation="MDEKMIEISFGIIGFAGDAKGLAFEAIKEAKSGNIDKARECIEK
                     SKESIHKAHKFQTELIQNEANGNKTEISVILIHAQDHLMTAMNFQQLAEEFIDVYERL
                     ESK"
     gene            complement(36607..37071)
                     /locus_tag="KR250_000275"
     CDS             complement(36607..37071)
                     /locus_tag="KR250_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016208838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0066247.1"
                     /translation="MVHLVYCDNAGKKGEKVLDKILDGTKTMVIRGAAGRKIPHSRVF
                     EDELLYFMEKGTAKISAKATVKKVENYVKLTEDEISKILNENQPKLNLSEKQKVRWHK
                     KCLCLVEFENVEEITPLDFDHQGNMDDWLIVEKIEDVVVGTSIPYNYEKSKF"
     gene            37341..38279
                     /locus_tag="KR250_000276"
     CDS             37341..38279
                     /locus_tag="KR250_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBH0066248.1"
                     /translation="MKKIKSLAIFISIITTLVLVTACSDKNTEDKDKSETRVVQSVKG
                     EVKIPSNPKKIVDISGSSEELLLAGYKPVATANVDSYETDKLPSYIREELKGVKIVGH
                     SMMDTMDMEAILEVNPDLIIMSQRQEKIYDQLKEIAPVVMMKDYANDWRSKLTDVSKL
                     FDKEEEDKSWLQKYDEKATKLGKEVIEKNGEKTYLPVLASSGQFMVFSDGGIGTLIND
                     DMKLARPKNMPKQDGITLPMVSMEGLTDIDADHIVVIATEADKKDLENSAIWAQIRAV
                     KDGNVTILDAAPFFSQSYNPIGKELLLESVKNELTK"
     gene            38407..39420
                     /locus_tag="KR250_000277"
     CDS             38407..39420
                     /locus_tag="KR250_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBH0066249.1"
                     /translation="MTKLSKKNITYLLILVSIVVLVFGIVLSITIGAKDMNLSTVIDS
                     LIKMEDGINMRIVKDVRLPRAIAAALVGGFLAVSGAIMQGITRNPIAEPSVIGITQGA
                     TFAISISLVLQKKLPQLIHGSFSVMMFAFIGASISGLFIYFISSKSRGRVNNVKLALA
                     GVALGTLLISLASAISMYFNLSQQLSFWISGGLVGVKWEGIKLLFVAGGIGFILAIIM
                     APRITILSLGEEVAIGLGQKTKFVRFICIVLVILMTGASVSVSGNIIFIGLIVPQIAK
                     GIVGSDYKYIIPSSLVLGAVLLVYTDILSRMINPPYETPVGSITALIGVPIFIYLVRK
                     GEK"
     gene            39426..40448
                     /locus_tag="KR250_000278"
     CDS             39426..40448
                     /locus_tag="KR250_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBH0066250.1"
                     /translation="MKNIIKINSKTLLVISILMVLIFITFLLSLNMGSFSIEPMDVIK
                     TFLGQGQRNHEIAIFKLRLPRIVIALLVGSALSTAGVILQGVTKNDLADSGILGINSG
                     AALFVVVYIYLMNGNVYEGMSNLTVFTMPIVALIGALFGAFLIYSLAWKGGINSSRLL
                     LIGIGVNIAFTSILTIFQLKFTTQEFNRVMAWTSGSIWGTSWKYVIAVLPFIVIFMGI
                     TIYKSKYIDVLNLGDEVSISLGVDVEKQRRRLIIYAVILSGVSTAVAGSISFLGLIAP
                     HIGRKIIGPKHKRLIPVSALIGTLLLLIADTISRNLLAPIEIPVGIVVSIIGVPYFIY
                     LMLVND"
     gene            40463..41245
                     /locus_tag="KR250_000279"
     CDS             40463..41245
                     /locus_tag="KR250_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004460318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0066251.1"
                     /translation="MNCINTKNLNVSYGNTDIIKNLNLNIPKGKITTIIGANGCGKST
                     ILKTIGRIISPNSGKIYINGEDIYKKKSKEIAKDMAVLPQSPQAPSGLTVSELISYGR
                     FPHKSGFGNSSKEDREIVNWSLEVTGIAEFKDRNMEDLSGGQRQRAWIAMALAQETDI
                     LLLDEPTTYLDLAHQLEILKLLDELNKKQGRTIVMVIHELNNAARFADHMIGIKKGKI
                     VCEGDAYDVMTKDNLKELFNIDAEIATDPRTNKPICITYDMV"
     gene            41257..42597
                     /locus_tag="KR250_000280"
     CDS             41257..42597
                     /locus_tag="KR250_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBH0066252.1"
                     /translation="MENQEALRHEKIWILLFRYSIPAIIAMMVTSLYNVVDRAFIGSM
                     EGIGSIAIAGLGVTMPVFTLIIAFGMLVSVGASTRLSIKLGERNREEAEKILGNALTL
                     SIIISLIITILGLVFLEEILFILGASKDSIFYAKDYMSVILVGSIFNLVAFSLNNAIR
                     AEGNPKLAARTMIVGCVLNLILDPIFIFVFNLGIKGAAIATVLCQIVVFFWVIHYFIR
                     GKSNLKLKKCNLKLNKDIVKKVFAIGMTPFAMEVAISITHMFTNNSLKVYGGDLAIGA
                     MTALTSILLMFMMPVFGLNQGMQTIISYNYGAKQYERAKKTLILSIIVSVVILSFGFL
                     VVQVFPEVFVGIFNKDSDLMDIAVRGISLNLITLPIMGISIVGPVYFQCISKVKHSMF
                     LTLLRQFILFIPLIIVLPIQFNLDGVWLAQPVADFIAMIIVLLFLKREFKTSRV"
     gene            complement(42692..44971)
                     /locus_tag="KR250_000281"
     CDS             complement(42692..44971)
                     /locus_tag="KR250_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBH0066253.1"
                     /translation="MNFENTKNKKIIATLLLTLLLIVSLTCVTFIMNLNKVTHNETER
                     YLVEVSQGISTTINVKCKSIFRTLYSMGDTYFEINDSEKEVNRYLSKKLKLFEFDWLG
                     IIDSNGNFVSALKDTGNVKGQDFYRKALQGKETITPVKSQSGTYGILYSVPLYNGDKI
                     VGAVAAWNTIDTMRNLLSVESFHGEGFSQIIDQDGNFIVSSANKNAPKNVVNFFDMIK
                     HQGKQVDKNKLEEMKINLKKNKTGSMYYTLNDGISKAMHYLPLQEENLYLLSIVPTKT
                     ASAQFDLLLKKAIFINILIVILFLLLIILIMFINQKNRAQLIKIAYVDSVTKGYSKAR
                     FDIEAKKIIQSSSSESYSLVSINIQKFKLINDSFGSEAGDKTLKYIHDVISNYLEPGE
                     LLCRVSNDQFNVLIKTIPQKEILKYIENITNDINSFNKNEECKYFIALSIGVYKIDDP
                     NLPLVSIQDRANVARKNNKGNIGNHPLYTCVFYSDLERLKMIKEKEMENRMEEALNNN
                     EFIVYLQPKISLKDNTIVGAEALVRWQDPNKGLIPPDEFIPFFEKNGFITKLDHYVFE
                     KVCSMIRKWIDNGETPIPVSVNFSRANLNNIDFLEKYRMLRNKYNIPAKLLEIELTET
                     LVFENLQKLINIIDQIHEEGFQCSLDDFGSGYSSLNMLKEIKVDTLKLDRAFFSSPNA
                     DNIAENHVIESIVNLAKKLNMNSISEGVETILQMDYLKKINCDMIQGYVFSKPLPQEI
                     FEKMTFGKKLEETSMDSSV"
     gene            complement(45308..46471)
                     /locus_tag="KR250_000282"
     CDS             complement(45308..46471)
                     /locus_tag="KR250_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HBH0066254.1"
                     /translation="MKLMGYLRENGQFGIRNHVLIIPTSVCSSETATRIASLVPGAIA
                     IPHQHGCCQIGSDIELTAKTLIGFGKNPNVAAVLVVGLGCDGIQAKELASEIATTGKK
                     VDYVVIQECGGTLKTVSKGAEIVSKMAREVSKEVRVEFGMSEITLALECGGSDPTSGI
                     ASNPSIGVASNLLVDEGGSSILSETTEVIGAEHLLATRFEDEEMKDKFLKFVSDVEKR
                     AIAMGEDLRSGQPTPGNKTGGLSTIEEKSLGCMYKAGNKPFKGALEYADIVPPDKKGL
                     YFMDTPGQDIDSITGMVAGGAQIVIFSTGRGTPTGSPISPVIKITGNSDTYNKMPDNI
                     DINAGRIITDGAKIADIGQEIFNEIIEVCNGKHTKAESLGHREFGIYRISSTF"
     gene            complement(46484..46759)
                     /locus_tag="KR250_000283"
     CDS             complement(46484..46759)
                     /locus_tag="KR250_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HBH0066255.1"
                     /translation="MNAIIHANEKDNLVTCVRPLVKGEKVSIDGKSYIVNDNIPVFHK
                     MATEDVKKGDVVYKYGEIIGIATVDIKTGDYVHVHNIESTRGRGDKK"
     gene            complement(46780..47754)
                     /locus_tag="KR250_000284"
     CDS             complement(46780..47754)
                     /locus_tag="KR250_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate permease"
                     /protein_id="HBH0066256.1"
                     /translation="MHILKSVKKIPGGLMVCPLLLGALFNTVCPQALEIGGFTTSLFK
                     TGAMSILALFCLCNGAQINIRQAGKPLTKGIVLTATKFIIGAVLGIIVSKFIGPKGII
                     GITPLAIVATMTNSNGGLFAALAGEYGDSTDVGAISILSLNDGPFFTMLAFGVTGLAN
                     VPIIALFAALIPILLGFILGNLDEDIREFLAPGTTLLIPFFAFPLGAALNFNQIITAG
                     LPGVVLGLGVTIITGMGGYFVMKLIRAEHPAVGAATGTTAGNAAGTPEALAAIDPTLA
                     TIAAVSTVQVAAAIIVTAICCPMLVSYLDKREKKKKSIFKTNKNIEVN"
     gene            complement(48025..49938)
                     /locus_tag="KR250_000285"
     CDS             complement(48025..49938)
                     /locus_tag="KR250_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBH0066257.1"
                     /translation="MKYGGIIMKEILVIAPTKGTYEKSIHIVKKNKYTNIDVVFGNLK
                     EGIPLAEKSINHGTRIIISRGGTYNMLKATYNIPIVEIKVDAYDIIKSYKEVKNSNEP
                     FGIIGFNNVIYGFDIIEEILNKKITMIEIEKEEEIYDAIEKYRKKGINTYIGDTTVAH
                     IVKRLNCKGILIESREENILRAIQQAEQILEATKDEQKRRLQIEAMTDFVHDGIITVD
                     KNFKITLFNKSAEKIFGIKKENALYHNVVDVIPNTVLPQVVETMEPQIGEIQNIGSTK
                     IITNRVPIIVDGQIQGAVSTFQDSKEISSFEHTIRRSLLSKGLSAKYTFDDIIHASEK
                     TKDCIELAKKYACYDTPMIITGESGVGKELFCQSIHNYSKRKNAPFVAVNCAAIPPSL
                     IESEFFGYEEGSFTGARKKGKAGVFELAHEGTIFLDEISEIPLELQGRLLRVLQEKQV
                     MRIGGDKVIPIDVKIICASNKDLKNMMRNGLFRQDLYFRINILTLYLPSLKERKEDII
                     KLSEFFIHKYSSKYNKTPLIITPNIKKALLEREYEGNIRELEGMMERAVIVESFDSIL
                     LEKVSNEYTNNIVDKDNSDFECSNLDLKTLEKKYIYKIYNENNRNTTKTCEILKITRS
                     TLWRKLKEIEDDV"
     gene            complement(50115..51329)
                     /locus_tag="KR250_000286"
     CDS             complement(50115..51329)
                     /locus_tag="KR250_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HBH0066258.1"
                     /translation="MNQEYVKGNPIFTNINKVPRQFPYLTDDIDTDVIIVGGGVTGCI
                     CAYYLAKNNIKSVILEKGRIAHGSTSVTTSLLQYELDDNLIDLTEVMTLKDALKAYNL
                     CTSALEELDTFIELYGNKCDYAKRDTLLYTANKLEVKAIKEEYNLRKENGFDVEYIDE
                     STNPFNFDLKSGLIAKNGGRELDPYKYSHHLIDVSLKNGLQVYENTEVKKVDLSNDKV
                     TAEVSYGYKVHGKKLIVATGYNTSLFTKRNFATKSNTFNIATKPLKNIASWKNNILIR
                     DNCDPYNYLRTTKDNRLIIGGEDVSFDDIENETLANEKYDILEQRLKSMFKDIKDIEV
                     EYKYCGCFASTLDNLGFIGPDNKNNNLWYCLGYGANGILFAILGGIMLSELYLGKQNK
                     NMKLFKVDRFDK"
     gene            51573..52955
                     /locus_tag="KR250_000287"
     CDS             51573..52955
                     /locus_tag="KR250_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011967718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HBH0066259.1"
                     /translation="MLSKNIASKVLEKCLITGGDFAEIFEEDTINNSISLIDNKVENA
                     IGGRNYGIGIRIFKGLKSIYAYTSDNSLNSLLDVAYKAAVALGKLEDGKSVILNDSIK
                     INNIHNIKIYPNSIGNKDKVSVMKVAYKSAKEFSNDISQVSVSYLDKDQKILIANTEG
                     IYVEDRRIRTRLGISSIASKGNENQTGFEGPGGCKGFEIFEEIDPEYYAKESARVAHT
                     MLHAKNCPAGNMMVAIDNGFGGVIFHEACGHSLEATSVAKGNSVFADKLGQQIASTKI
                     TAIDDGTIPNYWGSMNIDDEGNPTQKNILIENGILKSYMIDKLNGRRMGMNPTGSSRR
                     QNYKFAPTSRMTNTYIAAGEDRPEDIIKSIPDGLYAKKMGGGSVNPVTGEFNFAVSEG
                     YLIKNGEIQEPVRGASLIGKGSDILMNIDMVGNNVKQAQGMCGSSSGSIPTNVGQPMI
                     RVKEITVGGR"
     gene            52967..54316
                     /locus_tag="KR250_000288"
     CDS             52967..54316
                     /locus_tag="KR250_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HBH0066260.1"
                     /translation="MELKNFKDILFKKALSEGFEEYEIYYYTGENLSINIYEGEVEKY
                     NLDKSFGLSFRGKVNGKIGYSYTEILDDKAVDMLIKNVKDGVNTIENEDVQFIYEGDK
                     HYNDVKTYSKELENLEADKLIDLALEMERETKAYSDKVVNLSKCTISYSVSSNGISNT
                     KGLNLSNKTNMLIGFVVPIIEDNGQKYDGIGYNIANSIEEIKPCEIAKLGVDNALSKV
                     GGKSIPSGKYKTILLNEAMVSLLSTFSGIFNADSAQKGLSLLKNREGDMIASPIVTIV
                     DDPLLENGMASTPFDDEGVATFKKEVVLNGKLITLLHNLKTANKAGVKTTGNGIKSSY
                     SSPISIYPTNFYIEKGDKSLDEIIKDIDEGLMVTSFAGLHSGANSVTGDFSLAAKGFY
                     IKEGKKVFPVEQITVAGNYFDLLKDIEAIGEDLEFPMSSIGSPSVVIKELSVAGKDE"
     gene            54466..54999
                     /locus_tag="KR250_000289"
     CDS             54466..54999
                     /locus_tag="KR250_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HBH0066261.1"
                     /translation="MSDAHYDMHKRNGYSSDEPYPEIKVLGPNKYYAELLMDDYAGVS
                     SEFTSVNQYLYHNFDLDETHRELSEMWINISITEMLHMEILAKTIRLLGGNPVYRGST
                     SSCGAYWNGGFVCYGNSICNRLKLDLHLEHVAINNYYKDISLIEDPYIKAILNRIILD
                     EKLHVSLFEKAIEKYCK"
     gene            55083..55892
                     /locus_tag="KR250_000290"
     CDS             55083..55892
                     /locus_tag="KR250_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBH0066262.1"
                     /translation="MYYVYVNDINIAVYDLNPSAKKTVLFIHGWPLGHKIFEYQTNIL
                     PKLGYRTVSIDLRGFGKSDATSGGYTYSQLADDIYKVVHAIGLKDFTLVGFSMGGAIV
                     LRYMSLFNGYGVSKLVLAAAAAPSFVQRPPEFPYGMTREDVNKLIAQACTDRPEMVTD
                     FGEKVFASNPPESFRRWFNDIGFSASGIGTIGTAVSLRDEELFNDLKCVRVPTGIFHG
                     KLDEICPYEFAVFMNEKIEDSILYTFEYSGHAIFYDELKLFNQKFLQFLEE"
     gene            56303..58348
                     /locus_tag="KR250_000291"
     CDS             56303..58348
                     /locus_tag="KR250_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBH0066263.1"
                     /translation="MKKHNILFVSTDDKINIDISKQLENIFGEFCNIDNLVYVNRINI
                     ELSSYELVVCSDNDIKEYIHNNIDKNIPIVIVHRTINIENINQIISIENDSDVMVIDA
                     YKESADETAKIIRKLGLIHINLIPYYPGCDKSKCEIGIITGSRNSIPQNIKQIIDIGD
                     KVIDINTVIEIFTKLNISIDKLHIIKEKYNEDTVSGYRYYTTMNKTMKSFLEIIDEGI
                     ASVDKLGKFIYCNKVFSNLVGIDQNEIISNNFMDLFSDKVVKKIFFQEDEVNDEVVNL
                     NNKKLIINKVNVYENNERIKSIISIKNISAIQVLEDKIQNKLQSKGFVSKYTFESVVG
                     ESKIIKEKINIARKIATTDFSVLILGENGTGKEIFAQAIHNESLRKNKPFVAVNLSSL
                     SDTLIESELFGYDEGSFTGAIKGGKMGIFERAHTGTIFLDEIGDISLDVQQRLLRVLQ
                     EKEVMRMGGSKIIPIDVRIIAATNKDLKKKIKEGSFREDLYYRINVLHIEIPRLRERK
                     EDIPLISKYFLDEINSNKCFTEESMQALTLYEWPGNVRELKNLIYYIDTIVEEDRVDY
                     EHLPEQFRFEKNNTLVNENFDSIILDFKQSNFFEESICILTSIETWNNKNILLGRNKL
                     QEILKEKDIVLSVDQIRKRIDKLKSHGLLLSGVKKQGSFITDEGKNFISYVKIKGVI"
     gene            58609..59808
                     /locus_tag="KR250_000292"
     CDS             58609..59808
                     /locus_tag="KR250_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HBH0066264.1"
                     /translation="MKSAYKGYEAYSYLEKGKDISSIEMCKGDKRVKEYLIELDDIQE
                     AKVKSIVDEKIFISLHEHPVLFPEHLERDIFEYNREGKQRCAYEALSRGYYDAIFDNL
                     MDGVCTITSNSGWKWDDILIDLGMRLCDLAHQDFVIKCEKVEDIYRAKSEGKVALIPT
                     LEGAAPIENELDRIDILYGFGIRLMGITYSESNALGSGLKEEKDGGLTNFGKKAVERM
                     NKIGMAIDCSHVGVQTTLDVIEYSKDPIILSHVGARSLWNTKRLTPDEVLIACAKKGG
                     VIGVEAAPHTTITEKNKEHSIESFMEHFEYIKNLVGIDHVAFGPDTVYGDHVKLHSVL
                     AAKFDIKKITGTKEHPKVEYVKGLENPTETSKNIVRYLVKHGYSDEEIEKVLGGNILR
                     VLKQVWK"
     gene            59860..60555
                     /locus_tag="KR250_000293"
     CDS             59860..60555
                     /locus_tag="KR250_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5058 family protein"
                     /protein_id="HBH0066265.1"
                     /translation="MDYLKLANHPLLWIASTVAVSIVVVQSLIFAIKSCKVGKTMGIT
                     DKQIKSAVKSSAISAIGPSMTIFAGMVSLIVTMGGPIAWMRLSFIGSVIFESMSAGFG
                     TDALGITLGSPEMTKLAFTNAVWTMILGSLGWIIFTLLFGHKLDKITNVISSGKKSFI
                     PIISLGAMLGSFAYLNADRVLRFDNGTIACISGMAIMVILCMLEKKKNIKWLREWGLT
                     ISMFSGMIIASVI"
     gene            60568..61245
                     /locus_tag="KR250_000294"
     CDS             60568..61245
                     /locus_tag="KR250_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0066266.1"
                     /translation="MSKVKSIYNEEYLPFKIRYGRLTLSLGIIAALVPGIILSFGFGI
                     MPPISALLASTMAIVSMSAPNYIIEPVSYSPILGIPGTYMSFLSGNISNMRLPCSIAA
                     QKAAEVESGTEEGSIISTIGIAVSILVNISILTIGVILGGSVLSKIPAEVVEKLNLIL
                     PALFGSVFGQVFLQDKKLGLVAIVISVLTIILSKQGIIPQSLVVLICVFGTMLIARAM
                     YKDKLSD"
     gene            61356..62957
                     /locus_tag="KR250_000295"
     CDS             61356..62957
                     /locus_tag="KR250_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBH0066267.1"
                     /translation="MIYDLILKNGFIIGGTGNPGFYGDIAIKDNLIAKIDSKINSNTN
                     KEIDCCGKVITPGFIDPHVHEEIVAILDGKFEKFLKQGVTTTINGNCGHSITPYSSEN
                     VYEYMYKNGLLLEEEKKYLIDKNKCWNNFTEYCDLISKSGISVNMGFLLGHGTIRWSV
                     MGGSKDRPPTEKEKNEITDIINDGMKSGAFGISTGLAYIPSKYADIDELVDIAKQIKE
                     HDGIYTSHIRDYIGRYNAVKEAIEVGQKSGARVQVSHLSPVEIEAFDEILKARYNGVE
                     IMVDTVPRSSGHCMKKKRVIQFIMAISSSLFELGINGVMDALRNEEGRTLILKEAFIL
                     GDRGSIILLNTKDINMEKKSIREIATQKGIDEDKLLLDLLLDGDEELIFCLGGMYRAD
                     FPDKLHDDKIIDNPFVMVGSDCLFSVGGDMSWFELQRRGAFPIFFNMYRKSGVRLEEI
                     VRRVTSLPARQFKIKNRGILKEGLIADIAVIDMDNYSYSRSEDIDFSKPQLLANGVEY
                     VIVNGKIALENGEITENKCGEVLKR"
     gene            63406..64695
                     /locus_tag="KR250_000296"
     CDS             63406..64695
                     /locus_tag="KR250_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBH0066268.1"
                     /translation="MRELLKVTLIVMLVLINAFFVACEFAMVKLRSSRIDTMITEGNN
                     NAKRCKIIKDNLNSYLSACQLGITLCSLALGWMGESTVKELILPIVSVFNLSESIIYT
                     ISISISFLIITMMEVVIGELVPKALALYNTEKIMLSTSFLLVGFYKLTYPIIYFFNLS
                     TDLFLKPFGYSQADEVDEPHTGDEIRLLVEESYKSGLIDESEQRLVDNIFEFEEKKIR
                     EIMVPRTDMVCIYESDSEEKILAILKEEGVTRYPVCRKNKDDILGFVHIRDLYNQKIN
                     ENKIELEEILRDIIYISENLTIDKALERIRKEKLQLAIVVDEYGGTSGVVTIEDILEE
                     IVGEIQDEFDKEETHIKKIGAGSYLVDGTESINYINKYFGLDIEHDGFDSIGGWISYM
                     LGSNIKVNQSVTFENYNFTILELDKLRVVKLIIKSVI"
     gene            64724..65404
                     /locus_tag="KR250_000297"
     CDS             64724..65404
                     /locus_tag="KR250_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBH0066269.1"
                     /translation="MQKVEGIIFDMDGVLFDSERISLEFWMETFEKYGYTMTKEIYTS
                     VMGRNRKGIIEGLTDIYDSSVPIIDLYDEKTKNMIEFMERKGAPIKLGVNELISFLKE
                     NGYKMAVATSTKRERAVKRLAKANLKDYFDAIVCGDDVVNSKPNPEIFLKAAKKINVN
                     PKNCIVIEDSPMGVEAAYNGGIRCINVPDLKEPDEQIKSQSHKILENLLEVREYLKSL
                     NSKECHNL"
     gene            65505..66044
                     /locus_tag="KR250_000298"
     CDS             65505..66044
                     /locus_tag="KR250_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease"
                     /protein_id="HBH0066270.1"
                     /translation="MDNVGSLLYFLFNFFLYGFIGWIIENLYSYYKQGHFQEDGFLNG
                     PFKPMYAIAMSIIIEVSRFIDPNNIFALLLLCLVVPTVVEYITGILMKVYFNKVYWDY
                     SDYKYNSRGIVCLKFSVYWTILTFIGVRYFQVYIVDSLYSLIIPYWPIVVTILLVTLF
                     VDEILTIKFLRGNKDIVER"
     gene            66220..67239
                     /locus_tag="KR250_000299"
     CDS             66220..67239
                     /locus_tag="KR250_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBH0066271.1"
                     /translation="MMGKIRFGIVGTSNIANIFLRAASRVKDFELVAVYSRNLEKARE
                     FGTLYGASIFFDDLEQMAKSKDIDAVYIASPNALHSKQTITCLKHKKHVLCEKSLASN
                     LKEVKSMIKTAKDNNVLLMEAMRITCVPNFKAVKENLYKIGKIRRFFGSYCQYSSRYD
                     KYKNGIIENAFKKELSNGALMDIGVYCIHPMVNLFGAPKTVKAVSHILQTGVDGEGSA
                     VFQYDDMDAIIQYSKVADSYIPSEIQGEEGSIIIEKLNLFEKAIIKYRDGREEDISIP
                     KEKAEEKPREIEGMYYELVEFISLINNNKIESNINSHENSIIVMEIMDEIRRQSSIVY
                     PADSI"
     gene            67527..68717
                     /gene="dltD"
                     /locus_tag="KR250_000300"
     CDS             67527..68717
                     /gene="dltD"
                     /locus_tag="KR250_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HBH0066272.1"
                     /translation="MRKLIYFITPFIIGVVFLFGLDKFLDSKTDELLREKNLLPIMDD
                     TLSDIKDKGVTANNHFLREKDIMILGSSELSNSTKQHPKYYFNTNRSKNKVFAIGRAY
                     TQTLQDAAILGSMNPNIDNKKVVLLISMQWFMEKDGVTSHHYQSRFSPIQFYRFLDNP
                     KISKQNKIEYAKKSSKLLWGSDEYKAEALYAKLYEPKTFPEKAEKVLLEPYFQGRKYC
                     IALKEKGILYKRLIKLDKKRATKRKRPINWSYERKKAIEDAKKRVGKNPLNIDKYYYK
                     EHFKDGIEQYKGRDKDVNLLTSKEFENYKLMLNVCTDLGIKPVVVLIPSMDKFYNLTG
                     ISEKERNQYYDKAQKIAESKGFEVLNLKDKGSDKYYLRDVMHLGTKGWVDVCERLFKI
                     FKEQ"
     gene            68838..70352
                     /locus_tag="KR250_000301"
     CDS             68838..70352
                     /locus_tag="KR250_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase"
                     /protein_id="HBH0066273.1"
                     /translation="MKIIEGIKKYSNTDRTALMCNGDKLSYKDLNEYSDAISVFLKDV
                     YKEEDTPIVIYGNKENMIMACMIGALKSGRAYVPLDISFPIDRVFEVTKEIKPKVLFN
                     FSDERNFGDINVIDMDKLNYIINEYQGKSLDKENWVKDDENAYILFTSGSTGKPKGVQ
                     ISSNNLDSFSDWISPYLNIDGSEKVIMNQPAYSFDLSVTTIYPGLIHGATLFSISKDV
                     LADYKELFRQFSISDIAVWVSTPSFAGVCITEKEFNSKMLPNLESMIFIGEALSKNLT
                     KELMSRFPNTRIINGYGPTEATVGVSVNDMTQKAIDDEKSLPVGYPMSNCKIKILDED
                     GNELKENEKGEIIIIGPSVSKGYFNNKEKTDEVFFYDEIDGVKWRAYKTGDMGYLLDG
                     NIYYCGRKDFQIKLNGFRIEIEDIENNLRKVHNVKNAVVLPVYKDEKIAYLKGIVELN
                     EKNDLSNIKNGMIIKKELGKYIPSYMIPRNISIISEFPTNINGKIDRKKLMEEI"
     gene            70352..71500
                     /gene="dltB"
                     /locus_tag="KR250_000302"
     CDS             70352..71500
                     /gene="dltB"
                     /locus_tag="KR250_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HBH0066274.1"
                     /translation="MIFSQYGDYFYLYILLLTSIPAVILGLMGKNIKYYGMLASLFMI
                     FLIVGIDVQLKYLVIFIILEVIIVKGYEYVRRKTKNKYIYWGFLFASMLPIIINKISP
                     VTSFGIIGFIGISYLNFRTIQMVIEIYDGAIKEVKISKMLYFMLFFPTLSSGPIDRSR
                     RFEQDLEKQISRKEYIEEYLLPGIKNIVMGVGYKFVIAFLINTYWVSRIPKDMSFINI
                     LSYMYAYSLYLFFDFAGYSLFAIGTGYIFGIQVPINFDKPFISKDMKEFWTRWHISLS
                     RWFGDYIFSRFVMSSMRKKRFKKRTTAAHVAQMITMITMGFWHGLTWYYVAYGVYQGL
                     ALVLTDIYQRKSKFYKKHKKDKWFERVQIFITFHIVCFGLLIFSGYLA"
     gene            71519..71749
                     /gene="dltC"
                     /locus_tag="KR250_000303"
     CDS             71519..71749
                     /gene="dltC"
                     /locus_tag="KR250_000303"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HBH0066275.1"
                     /translation="MQETVIEIFEDVLGTDEIREDLDLNLFETELLDSLAIIEVLLEI
                     ENRLGIELQPTDLERKDMSTVNNLVKFLETRK"
     gene            71966..72751
                     /locus_tag="KR250_000304"
     CDS             71966..72751
                     /locus_tag="KR250_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBH0066276.1"
                     /translation="MSLNSIDRKKEILKLLDSKGKVNTKELVKRLEVSSETIRRYLEE
                     LENENKLKKVYGGAIKINYGVIEPEHIKRNNTNLDKKQSIAKYAAKFVNDNESIIIDE
                     GTTNLQIVDYIISVNNLKIITNSYPVLSKLISYENQGLFDGEVIFIGGKVNLKHQRTA
                     CPISIDAMKNLYVDKAFISSEGILDSFGISSIDQNKALLSKAYIKNSKESFVLCDSSK
                     IGIASMYKIADLEELDFVICDVEPPNEWKDKLNEINITWIEAK"
     gene            73030..74940
                     /gene="phnW"
                     /locus_tag="KR250_000305"
     CDS             73030..74940
                     /gene="phnW"
                     /locus_tag="KR250_000305"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HBH0066277.1"
                     /translation="MKKIYGEKIKAVVFDWAGTTVDYGCFAPLNVFIEIFKRRGIDVT
                     MEEARKPMGKLKIDHIREMCEMDRIKNIWSDKFGKVPTEDDVNELYAEFEPMLFETLE
                     EYTTPIPHVVETIEKLRKNGLKIGSTTGYTREMMNIVEPNAAKKGYSPDFLVTPSEVS
                     QGRPYPWMCYKNAEALGVSPMSSMVKVGDTISDVKEGVNAGMWSVAVIKGSSELGLTQ
                     EEVENMDKEELKAKMSIVSKKFKEAGAHFVIETMAELEDILIKIENETIKSDFVPEND
                     YILLTPGPLSTTKSVRASMLKDWCTWDVEYNNLVQDVRRRLVSLATQNTDKYTSVLMQ
                     GSGTFSVEAIIGSTISKDGKLLVIANGAYGKRMKDICNYLNIQFVDCTFKDIEAVDLN
                     VVENLLKENKDITHISMVHCETTTGRLNPIQEVGKLAKEYNKIYIVDAMSSFGGIEID
                     VEDFNIDFLVSSSNKCIQGVPGFGFIIANKEKLSKCKGIAKSLSLDVYAQWETMEKNN
                     GKWRFTSPTHVVRAFYQALLELEEEGSVEKRYARYKENQFTIASRLKSLGFDTLVNDN
                     AQSPVITTFLYPKNAKFEFMEFYTYLKDNGFVIYPGKLTDIDTFRIGSIGEVYPTDME
                     RLADVIEKFINR"
     gene            75299..76120
                     /locus_tag="KR250_000306"
     CDS             75299..76120
                     /locus_tag="KR250_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0066278.1"
                     /translation="MNEELSSLRFDGVGKCKGGIFDNVNISGTGKIEGSVKCSKFDSS
                     GSAKVFGTTECNEFFTSGLSKVEGNILASKVEISGLLRCTGNINSVEIDTSGIINVEG
                     SIKSDIIHGEGYLKANENIDCENINLSGILECNGFLNCEEVNISLNGTSNFNEVGASS
                     INIKKGHGDYCKKFRMLEKKHINFEKVSSNLISLGLLSIRKYTESKLFANIIEADEIS
                     LENSEVKVVRGKNIKIGKGCYVESLEYSESIEIDEDSIVGEIKNISEKIKLKKDK"
     gene            76161..76928
                     /locus_tag="KR250_000307"
     CDS             76161..76928
                     /locus_tag="KR250_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HBH0066279.1"
                     /translation="MEKGSIKILGEGFISSGEYENIKIVGSSKSKGNVKSNEVKVLGD
                     AKFEGDINIKNFVVNGKTIIRGNLKAERVRVNGQLNIEGDAEIDDLVITAQQKVDGKL
                     KCNNVTIRGQLSVNENMYAQDIKIYGEMRNKSNIECEDIKVYGAIKCDGLLNGENIYV
                     YSGGGSYCKEIGATNIKIGRSKDAFSLIKIINIFSGKFKCNLIEGDTIELEDASIKDI
                     RGKSISLVDNCDVGNIEYSDNLSVSDNSTVKNSIKVG"
     gene            76936..77556
                     /locus_tag="KR250_000308"
     CDS             76936..77556
                     /locus_tag="KR250_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbD family protein"
                     /protein_id="HBH0066280.1"
                     /translation="MEELISKKDLLSKTNISYGQLYRWKRKNIIPEEWFIKKSAFTGQ
                     ETFFPRDKILERIELILSMKEETSLDDIANMFMKKDINKEFDIDFILSKEVICNYTKE
                     IFQNLYKKEQSIGKKELLILSIIEKFLVKSIITFEELKLIVNIIEENFTSIYNESGKI
                     YLFRKFGVPFVVGCLDYKKVKFEKDVVKIIEIDLTKEINDISIKLS"
     gene            complement(77856..78443)
                     /locus_tag="KR250_000309"
     CDS             complement(77856..78443)
                     /locus_tag="KR250_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HBH0066281.1"
                     /translation="MNLKDSKTKENLLRAFAGESQARNRYNISASVAKKEGLYIIESL
                     FNYTADQEKSHAKQFFSKLKEFAGENISISGAYPVDLYDKTAQHLKAAQHNELQEWDD
                     VYKNFGKTAREEGFESIAKLFENIAEIEKVHGDRFGRYATDLEEGTLFRRNEDIQWIC
                     THCGHIHIGKEAPKACPVCLHPQGYFLDFKNSLFE"
     gene            complement(78529..78966)
                     /locus_tag="KR250_000310"
     CDS             complement(78529..78966)
                     /locus_tag="KR250_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBH0066282.1"
                     /translation="MEDKYIVHFISKTKASMIKFIENKLSKNGLGELIPTHGNILTAL
                     YENNGILTMKEIAAKIGKDKSTVTVLVNKLINLGYLERQKCTNDKRITYIKLTKKALL
                     IEDTFNSISTQVKETAYHNITENEKQEFLRILKKINDNFKNAD"
     gene            79070..80068
                     /locus_tag="KR250_000311"
     CDS             79070..80068
                     /locus_tag="KR250_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdH/YhfP family quinone oxidoreductase"
                     /protein_id="HBH0066283.1"
                     /translation="MNKKFKSLYITEEEGNFRRNIVEREVDTLPEGEVLIKVKYSSLN
                     YKDALSANGNKGVTRKYPHTPGIDASGIVESSSCDDFKIGEEVLVTGYDLGMNTSGGF
                     SEYIRVPSSWVVKLPKNLSLKEAMDYGTAGFTAALSVYKFIDSVDKNMGDVLVTGGTG
                     GVAVIATKILIKLGYSVVVSTGKLEEQKEVLLNLGVKDVIHRSEVDDNSGRPLLRPRW
                     AGVIDTVGGNTLATAIKTTNYCGAVTTCGNAGGVDFTSSVYPFILKGITLYGIDSVDC
                     PMNLRLKIWDLLSNEWKLDDLDDMVNVVSLEELNDSIDSIMAGKHVGRTVVDLEKI"
     gene            complement(80188..81114)
                     /locus_tag="KR250_000312"
     CDS             complement(80188..81114)
                     /locus_tag="KR250_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HBH0066284.1"
                     /translation="MSKENKNVRIAVVQASPVIMDLEKTVEKALGLIKEAGRKGANIV
                     VFPEAFIPAYPRGLSFGFVVGSRTMEGREDWKRYYDNSVPVPSATTDLLGKAAQEAGV
                     YLSMGITERDGNDINCTLYCTNLFFSPEGKLIGKHRKLKPTGTERCIWGEGDGSTLTV
                     VDTPYGKMGSLICWENYMPLARTALYAKGVKLYIAPTADSREEWQATMKHIALEGRCF
                     VIGCNQYVEKNTYPTDLNYYKELDAEPEIMCPGGSCIVDPFGKYVAGPIFNKEEMLIA
                     DLDLEKIVLSRLDFDSEGHYSRPDVFELIVHE"
     gene            81500..83104
                     /locus_tag="KR250_000313"
     CDS             81500..83104
                     /locus_tag="KR250_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBH0066285.1"
                     /translation="MKAKIYYNGNIITMEDSICGDAILIKDKIIKKIGTKEEVFALKN
                     KDTEIIDLQGKTLMPSFIDSHSHLIAFATTLKLVPLEDATSFKDIVKKIQDFKESNNI
                     KKGDWIIGFSYDNNFLEENKHPDKSVLDSASSENPILISHASGHMGVANTLGLKQLGV
                     TNETRDPEGGHIGRVEGSEEPNGYLEENAFFNVASKIKQPSSNEIFNSIEKAQNIYLS
                     YGITTAQEGLMEENQFNILKAMANQNKLKMDVVGYVNLKKSKSVADNNREFIKKYINR
                     FKIGGYKIFLDGSPQGKTAWLSRPYENSDDGYCGYPIYKDEEVEKFIDISLKEKMQLL
                     THCNGDAAADQLIDAFEKVLNLKEQSSENNIRPVMIHAQTVRADQIDDMKVINMIPSY
                     FVAHTYYWGDIHIKNLGEDRAFKISPLKTTIEKGLIYTLHQDTPVIAPNMLETVWCAV
                     NRITKKGIQIGENEKISPLDALKGVTINAAYQYFEEDKKGSIKEGKLANLIILDENPL
                     TIDPMKIKDIKVLQTIREGEVLYSLK"
     gene            83920..85191
                     /gene="serS"
                     /locus_tag="KR250_000314"
     CDS             83920..85191
                     /gene="serS"
                     /locus_tag="KR250_000314"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HBH0066286.1"
                     /translation="MLDIKRIRENLDCIKKAMERRGEKNFNLDEVVKLDDERRKTLQE
                     VEVMKNELNTASKNIPNLIKEGKDVENEKIKLKELSDKIKVIDQNLKEVEDKMEYLLM
                     RIPNVPHPEVPQGETDEDNVEVRTWGKTTTFDFESKAHWEIGTELGILDFETASKITG
                     SRFTLYKGLGARLERALLNFYLDTNTKVNGYTEVIPPFMANRNSFLGTGQLPKFEEDM
                     FKIEGMDYFMIPTSEVPLTNIHANEILKFEQLPINYTAYTPCFRSEAGSAGRDTRGLV
                     RQHQFNKVEMVKIVAPEESYNELEKLTNNAETMLQLLNLPYRVVKICTGDLGFTASFK
                     YDVEVWMPSYNRYVEISSCSNCEDFQARRAGIRFKRDKDSKAEYVHTLNGSGLAIGRS
                     VAAILENYQQEDGSVVVPEVLRPYMGVSVIK"
     gene            85559..87064
                     /locus_tag="KR250_000315"
     CDS             85559..87064
                     /locus_tag="KR250_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBH0066287.1"
                     /translation="MKIQNTEWGYIEWKHTYDENNPKQAMNIYIAVTMPGKKHFNHVH
                     YGQEQMIYILEGEGLYIINGVWKPFYQGMIFYIESGSTHETINTGDREIKELIVSNNV
                     DDVGESEVIDINPNNYLKKTLINYSESTLNLYAAVESIRGQFIDPFKIPLIIYDDSWN
                     IVLKNPYFPLFCFEKCNPMKFPQNCDCMNQKSSNQFVCEYGITIYNIPILYKSNSIGV
                     IRGGYVLLSDLNLDTEHNNLYDIPEGAARSIKRLLKQISKNIINFCSFNDIRKDLQEK
                     EKTIARTYHYGEQLEMNLKVAQDMVTNLRINHHFLFNTLNSMASIALDDGSYDLYSAI
                     IDLSRMFRYTMRSDLRFVELESEILYIKNYLNLQKLRYGDALKVKYLIPEKLYNLSVP
                     FNFIQPIVENAFTHGFRDIDTEKRIEIIARLDSQYAIIEIHNNGTILDGNNIDKIKAG
                     IRSNNGHGLSLIYTKFTSAYGNNFDMDIKSSDNEGTYIMIKIPIENYKEYV"
     gene            87178..87876
                     /locus_tag="KR250_000316"
     CDS             87178..87876
                     /locus_tag="KR250_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBH0066288.1"
                     /translation="MRRALIIDDEEAVLKIITHFIEMKDMPIKIVGKATSGDEAVDKI
                     IGLEPDIVFIDIQMPIYNGLEVIEKTSHLSKKINFVVITAFNYFEYAQKALRLNVKDI
                     LLKPLDMKEFTKSVEKIIGYQYTNNDLLNEILEYINLNYSNDLKLNDCARLFLTNPSN
                     ISRIFKSNLNTTFISYLNHIRIEKSIELLENTTKSINEIAEIVGYNSLNNFYKNFKIE
                     KGMTPKVYKLNTKN"
     gene            88161..89249
                     /locus_tag="KR250_000317"
     CDS             88161..89249
                     /locus_tag="KR250_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0066289.1"
                     /translation="MSDKVNMKNIICIAGAYLATAIGSGFATGQEILQFFASQGYMGL
                     IGALISMVLFSFLGAEVITRGRELKLKEPTRIYTFYCGKILGKFYEWFSPIFLFGILI
                     VMISGAGSTLTEYYGINPYLGRVLMAVATLITVSMGLEKLVSILGRIGPVIAVFTIAV
                     GLISLFKHFDGLSMVGEAMKNIEVTKASESPIVSGVIYNTLNIIVMIVFLTGMGASIE
                     SKKDAFWGGLLGGIVFMTAGIVMYLAMISDIGNLYMKEIPSLYLADNISPIVGVCFSV
                     ALILGIYTTAVPLLWSVTNRIVEDEHPKFKLVTTVIAILACIGGFLPFGKLVNILYPY
                     TGYMGLLILVCMVYKAITCRKKSTQSQE"
     gene            89488..90678
                     /locus_tag="KR250_000318"
     CDS             89488..90678
                     /locus_tag="KR250_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBH0066290.1"
                     /translation="MHELILISEKYKEDILDFFTDIHMHPELSFKEFRTTKAIKDLLV
                     SLDIEILDLGMETGVVGLLKGKYDGPTVALRGDIDALPIYEEVDIKYKSRYDGIMHAC
                     GHDIHTSCLVGCAYVLSHIRDSLHGNVKFIFQPAEEVNKGAKMLVERGVMENPKVDAI
                     FGLHNHPDIPCGKIGVKLGGLMAAVDTIKIEVNGFGGHGGIPNRTIDPIVASSAIIMG
                     IQTIVSRNISPLESAVISIGTINGGTANNVISEKVDMTGTCRSFSNEVRKKISENLEN
                     IVCEIARGYQATAKLDYLFDLPAVINSKEMYAIACKSVCDLYSEDAIVDPIPSTGGED
                     FSIFMEKAPGFFYWLGVGNKEQDCIYQWHNPKFKADKNSILVGTNVLCQSVINYMDKL
                     KNKI"
     gene            90769..92271
                     /locus_tag="KR250_000319"
     CDS             90769..92271
                     /locus_tag="KR250_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HBH0066291.1"
                     /translation="MRALNVVERAGNKLPDPVTIFLLLCIIVVILSAVISNLGVEEIH
                     PSTKEVVKVVNLLEKEQIQSYLGSIVTNFQSFAPLGLVLVTMLGAGVAEKSGFMEVLM
                     KKGISKVPQKLVTVAIVFAGMLSHTAADVGFIILPPLAALVFLGIGRHPLVGMFAAFA
                     GVAGGFAANVMLSTTDVLLAGFTIPAAQMMDPSYQGNPAMNLYFAFISAIVLTFAGAF
                     VTEKFIAPRFTKYNDSTNDKDIQELSELENKGIKYALLSLLVVVVVIVALCIGDNAFM
                     KDPETGSILSSNAPLMKGIVPIITIIFLTPGLVYGKVSKKIKSDKDLVSMMGSSMSDM
                     GGYIVLAFIASQFINLFNLSNLGTILSITGAKLLAESGIPSYGLIIGFILLSGFINLF
                     VGSASAKWAILAPIFVPMFMLLDFNPALTQIAYRIGDASTNPISPLFPYFPVILAFAR
                     RYDKDIGIGTVISNMIPYSVVFTLIEIIILLLFMGIGIPLGPGGGISYVL"
     gene            92343..93599
                     /locus_tag="KR250_000320"
     CDS             92343..93599
                     /locus_tag="KR250_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HBH0066292.1"
                     /translation="MLKTTKISDPELYKIVADELVRQEHNIEMIASESTAPTEVLELS
                     GCVFTNKTEEGYPGARFQAGSEEADKLETLAIKRAKEVFGAEHVNVQPYSGSTANYCV
                     YSSILKPNDTVLSMRLDQGGHLTHGSAVNFLHDIYKYEFYGVDPNTGRIDYDALEAKA
                     KECRPKLIIAGASSYPRLIDYERISKVAKEVGAYFMVDMAHVAGLVAAKVIPSPVPYA
                     DFVSSSTTKTFCGPRSGIVLCKAEHAKKLDKGVFPGTLGSIHLNTVAAKAFSLLYLST
                     DKFKKIMEQVVVNAQTLASELISHGFSIVSGGTDNHIVMVDLRSKNLTGKQFEKALEY
                     VGITVNKNVIPDDPQSPFVTSGVRIGLTSISQRGLKEKEVIQIAGIMNKVAENIDNKE
                     VLDECKAEAQELISKFPLYPEGYFED"
     gene            93771..96545
                     /locus_tag="KR250_000321"
     CDS             93771..96545
                     /locus_tag="KR250_000321"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HBH0066293.1"
                     /translation="MRYYNKPTKEVLKYLKTNPEIGLDDNEVEERKLRYGLNEFTIKE
                     GRTFWDELGESLTEPMILILIGAAVISSFVGELHDALGILGAIFIGISIGIITEGKSK
                     KAAHALSKLTENIEVKVLRNGKIIKISKNDLVPGDIVYIETGDMIPADGRLIQSINLK
                     LREDMLTGESDDVAKNADAVLDMEVVYSKTEIIEQDAIPAKQVNMVFGGTLVAYGRGI
                     MVVTHTGDKTEMGKIAQNLSNEDQQTPLQIKLGKLGAKIAGISGVIATLLCMFMIIQM
                     QRKGMLILDTSSVLSFLQSLEPAKNAYMVCIALIVATVPEGLPTMINITLAITMQKMA
                     KINALVTKKEACETIGSVSVICSDKTGTLTQNKMMVEVAYVDGKYISGGEYQSNSYFE
                     QNCIVNSTADIEKEDNSFKYIGSATECALLLYHNDKNYNEMRKQTYLISQIPFSSEEK
                     KMSTLIRQEDSDILLSKGAPEVLLKKCSYVQQGKNIVPITPKVEKSILDEIKKLQIKS
                     MRTLGFAYKKMSNSKTEVAMTSEGELNLIGNSRSYMKEDNLVFSGFVGIVDPLREGVK
                     DSIDKAFNAGVDVKMLTGDNINTATAIGNELGLLNDGKKAVEATYIDVLTDKELREEI
                     KGISIVARSKPDTKMRIVSALQKSGEVVAVTGDGINDAPALSQADVGIAMGISGTEVS
                     KNAADIILTDDSFSTIVEGIKWGRGIYENFQRFIQFQLTVNIVAFIIAIISQLTGKDM
                     PFTTIQLLWVNIIMDGPPALALGLEPVRDYVLKRKPINRHSGIIARSMFVNIIINAIL
                     IITIVFTQSAFNILGATSEEQGTVIFSLFAFSALFNALNCREFGLNSTIPNFFKNKLA
                     LQIIVVTGIIQIIFTQVFQSFFNSVSLDFDMWIKIILFASTILLSNEFVKLILRTMKN
                     SRTMNFNSKN"
     gene            complement(96646..96795)
                     /locus_tag="KR250_000322"
     CDS             complement(96646..96795)
                     /locus_tag="KR250_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HBH0066294.1"
                     /translation="MFFISYIYTKSPIEFDTPLQKEAYKILQKLDIDFECVDTDEAIT
                     MEDCV"
     gene            97170..98348
                     /locus_tag="KR250_000323"
     CDS             97170..98348
                     /locus_tag="KR250_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBH0066295.1"
                     /translation="MFSKILVKTFIRDSENVQNTDVRNKYGYVAGVVGILSNLLLFVI
                     KVFIGMLTSSIAIMADAFNNLSDMASSAITMIGFKLASKPADKEHPFGHGRIEYLSAL
                     IVAFMVMLVGLQFVKSSIERIVNPIPVKFEVIPLILLIASIMIKIWLSRFNKFMGNKI
                     DSSALKAVSLDALGDVFTSSCVVISFIVARFTNFPIDGYVGIVVSLVILYAGFSLVKD
                     TINPLLGEAPDEEMVNSIIELLLSYKYIIGTHDLIIHNYGVGRCIASIHAEIPSNIDI
                     MEIHEIIDTAEREISEKLDIYLVIHMDPICLEDKEVMSAKKELEEILKKNSLVKSMHD
                     FRIVGKGSKKNLIFDIVVNPSEFSKNMSEDDLKEDITKLVKEINPEYNCVIVVDKDFI
                     "
     regulatory      98475..98560
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            98678..101596
                     /locus_tag="KR250_000324"
     CDS             98678..101596
                     /locus_tag="KR250_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021362860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SrtB-anchored collagen-binding adhesin"
                     /protein_id="HBH0066296.1"
                     /translation="MKKGNRKALLISLIMILSMVVSTIYPTVSYASELGENSQIQSGS
                     TNSSTGEEKESDNKKPEQTPEKDKATDNKKPEQTPEEEKPTDNKKPEQTPEEDKSTDN
                     KKPEQTPEGEKPIDNKKPEQIPEEDKSTDNKKSEQALEDEKPLDNKNTEKTPEEDNLL
                     EDENLLKVLEEELNEENEDYGFVVKINNNTIETESMKKISFNLTYTPTSKGIQAGDSI
                     TFKVPDVFNKVNLDYTSECFDKTESNGEYTLTFRELPNGQSVMQGKIGLEAYVKKVDE
                     DTNAKIHIETTGKIESGSGDIDVEIKPGDKTDVPDAKGTLKKLVEGKKSTTVFMPVKN
                     KDINYSIQVNEKQEELKDIILYDELPEGLTLINGSVSVVTSDGKEVSDFNIEQSKNSI
                     SVNFGNIDKSYTVKYKARISDKNAKHGNKYKNVARIESDGKKIQEDDATVSIFDRGDD
                     YLLTKGHSGATNITQVGQVINYQISINDDKSPISNVVITDNIPEGMRLTTSGEAGHDF
                     RVVEIPMNGSWTPWSKEKIANNISYKVEEKRNESGQVDKVITGFTINLSKEEVESKFF
                     IAYTLKVISIEDSYINRAVLDANNSEIDKNDEINFKKNSGLISAKKEVDKKVLNSSDN
                     QIVKYKINMSTYGVYDAGQVNLLDEVNSALEISNIKYSDNLELKKEAGDGKNTIRLVN
                     KYEFKQIKEGEPVQSWVTFDADFTNVKVGETIQNVAQINGSSPPGVETTKQGYAFEAK
                     KVDALDKNVLSGAKFNLEDAFGNIVVKDLVSDEDGIIQSSIKKPGTYYLVEIMAPSGY
                     EKLKDKIKVEIGNKDIGRIVDIGNIENIRQENPPVNPPAPPNTDEPIVNPPVPPSTDK
                     PRKPSSSSDTEDTIVINPPIPPSEDIINPPIPEVLNPPVPPSEEMIETPVKQIIPIPE
                     VVKPSVSEEKNNKVKDDTLVNPPVPPKTGDSTTIIGEILLVIGAIVGLIVLRRNKNTN
                     "
     regulatory      101782..101867
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            101967..102629
                     /gene="zmp1"
                     /locus_tag="KR250_000325"
     CDS             101967..102629
                     /gene="zmp1"
                     /locus_tag="KR250_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease Zmp1"
                     /protein_id="HBH0066297.1"
                     /translation="MRPSKKLLIAIISIFLISSVPVSAHADSTTIQQNKDTLSQIVVF
                     PTGNYDKNEANAMVNRLANIDGKYLNALKQNNLKIKLLSGKLTDEKEYAYLKGVVPKG
                     WEGTGKTWDDVPGLGGSTVALRIGFSNKGKGHDAINLELHETAHAIDHIVLNDISKSA
                     QFKQIFAKEGRSLGNVNYLGVYAEEFFAESFAYYYLNQDTNSKLKSACPQTYSFLQNL
                     AK"
     gene            complement(102751..103713)
                     /locus_tag="KR250_000326"
     CDS             complement(102751..103713)
                     /locus_tag="KR250_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HBH0066298.1"
                     /translation="MKKGYVYIILTTLFFSSMEISLKTVTNDFNPLQITLSRFFVGGL
                     VLLPFAVKRLRALSLSITKSDLKYFAFLGFMCVVVSMSLYQLAVLNTKASVVAVLFSC
                     NPVFVMLLAYLILKEKIYRHNVISLILEVLGIIVIINPLHTKLTLSGIFLTLSSAIIF
                     SMYTVFGKRKTLKFGGIVVTCFSFIFGSLEMLILVLLTKINFIADILNKNKLHVFANI
                     PIFSGYTLHNMPIMIYVFVFVTGVGYALYFMAMEATSTSLTSLVFFFKPVISPILALL
                     ILKEIIPINMVIGILLIVIGSIISIIPTIITQMHNKHVEALYKE"
     gene            complement(103883..105073)
                     /locus_tag="KR250_000327"
     CDS             complement(103883..105073)
                     /locus_tag="KR250_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBH0066299.1"
                     /translation="MISNKMQTLVANSSVIRAMFEEGKKLSDIYGEENVFDFSIGNPS
                     VEPPETIKAVINDILNEESPNLVHGYMNNSGYEDVRDAIAEHINKKDGLNLTRENLIM
                     TCGAAGGLNIILKTLLNPGDEVIAFAPYFGEYKNYTENYDGKLIEVPTNIETFEPDLD
                     ALKNAITPKTRALIINTPNNPTGVIYSEELLKNLGELLDSKQKEFNTSIYLISDEPYR
                     EIIYDGAKVPCVLKYYRNSFIGYSYSKSLSLPGERIGYIVANGQMDDFDDVMSSLNVA
                     NRILGFVNAPSLFQRVIARSLDAEVDVNIYKKNFDLLYNSLIDMGYSCVKPNGTFYLF
                     PKAPIEDDKKFCNDAKQFNLLLVPGSSFGCPGHFRVSYCVSYDKVKSSLPAFEKLAKL
                     YNLK"
     gene            105379..105825
                     /locus_tag="KR250_000328"
     CDS             105379..105825
                     /locus_tag="KR250_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBH0066300.1"
                     /translation="MDFKNLQYESLSYIICNLQKNFKLYCEKCLKPYKLTNGLYFYLI
                     YINKNRNCSLNDVSTEFEVDKAHTTRTISRLEQDGYIEKIQNPNDSRAFQLRVTDKGE
                     EVLGDIKNIFSKWDNHIKKEFSDTEYKELVKNLHVVKDIKTAVEEE"
     gene            105827..106921
                     /locus_tag="KR250_000329"
     CDS             105827..106921
                     /locus_tag="KR250_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HBH0066301.1"
                     /translation="MYCNLLKPIKIGNLELKNRVSFAPTSMGLKLEEKIKKFSDIAKS
                     GVALITLGDVSIRPSFHKVAISLSDEDGVMKYKKIVDEIHNSGAKVSAQLFCSDYDVN
                     LIKDTMNMGITSHDEIKKIMNDGVKDYITNMPKEEIKNIINLFKVTALNAKKAGFDMI
                     QIHGDRLVGSFSSSIFNNRNDEYGRTCDNRSRFASEIISSIRDEVKDIPIDYKFAIRQ
                     ENPHYGNAGVLLSEVEYFVKKFESLGVNSFHVTLANHSKLEDTIPTNNHPYFKEEGCF
                     LYLADEVKKHTNLPVCGVGKLSSPDFIESIISNNRVDMVSMSRQLLADRNWLQKVKDG
                     RVDEIKKCCYCNKKCADALQTRSQFGCILD"
     gene            107009..107539
                     /locus_tag="KR250_000330"
     CDS             107009..107539
                     /locus_tag="KR250_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBH0066302.1"
                     /translation="MKSLVLYSSLTGNTKKIAYTIYDEIQEEKDIKDVNELVDYSIDY
                     ENYDIVFLGYWVDKGICDKNSKQVLENIHNKKIALFGTMGASKKGSYGASIIEKIESI
                     IPKDNEILGSFICQGKIAEGLKAKYKEMLKLSPDNEHIRQQLNNHEESQSHPDEQEIY
                     EASMFAKNMMIKASIV"
     gene            107866..108225
                     /locus_tag="KR250_000331"
                     /pseudo
     CDS             107866..108225
                     /locus_tag="KR250_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891108.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
BASE COUNT        41619 a        12495 c        18652 g        35884 t
ORIGIN      
        1 ctataaatat ttaaatctta aaatgttctc tttgagataa gtttatttca gagtcacaat
       61 ttccacaaca gtaagatgtt gagacatctt taaatttttc agtattctca tcaaagttca
      121 tttttttctt tgcaataatt cctaccttag ttaaattaga accacaatta ttacagtttt
      181 gaattttgca taggttatat agtttatcaa gtgtaatatc ttctaataat tcatcacaat
      241 tacaacatct gatagaagaa acttcactac acatttcaat aggttcagct tctttagaag
      301 aaaatttata tgttatgtca tttgatactt tataacctgt atcaaatata ttagaattac
      361 aacttggaca taagtacttt ggaagatata tatctttttt ttcaaaagta tcactactaa
      421 tattacagtt taattcatct aaaattggtc tttcatctag gttattacct ttatttaagc
      481 ttagaagata attataaatt tttttatctt cattctgttt tgatgtatct aacaaatgct
      541 ccattatatt agtgtatttt ttgttttttg aaggcatgtt taaatccaga taattgtttt
      601 caatagcaaa gcatactata ttgaaatagt tatatttaac tgcaaaaaat aataacattt
      661 catcaaatac tttatctatt attttgtcag aaatacttat ccaaaaatct ggtgactcta
      721 agtaaacttt cctactaagt tcatctaact cttcatctat attatttata ttattttcat
      781 taactttcat atcatcactt ccttttaaat atcttgtgta taaactattt gttactattt
      841 tttatataaa ataaaatctt atataaaaat atatcatatt ataatttaca agtatagtct
      901 aaatagagct gtttttaagc aaagttttat aaatgactat taaaatataa agtaacagta
      961 taaagtttac ttataaatta aatgtaaacc taaatgctta taatagttga caaaaaaacg
     1021 tctttatttt atactaaaac aaagctttaa tagaagcttt gtatatgtac atataaacta
     1081 aatgaaataa gacatatgaa gggaattata aaaatgaaaa atactaaagt aaatattcaa
     1141 gatttaacta aaatgtcaat atgtgtagca ttattgtgta tatcatctta catatatata
     1201 ccattgccat ttactccagc aggagtaaca gcacaaacaa taatgataaa tttaatagct
     1261 ttgattttaa caccaagaca agcatttagt acagttggtg tatatataat gataggttta
     1321 attgggattc cagtatttgg tggaggtaca tctggtattg gaaagttatt aagcccaaca
     1381 ggtggttttt attttgggtt tttgtttgca gcactaatta ttagtttatt aaaaggacga
     1441 aataatgata ttaagagata tatactaata acaatttttg ttggaatgcc tattatatat
     1501 tttatgggaa cagtatttat gtgctatttt aatcaaatga cagtagaagc agcacttttt
     1561 gcagcagtag taccattttt aattggagat acaataaagt cagttattgc atctttttta
     1621 ggcactaaac tgaacaatgt actcagaaga aatctatgat aaatctataa tataaggaag
     1681 ggtgtaatgt atgaaggaag tttttatatt aggaggattg agaagtcata taggtctaaa
     1741 aaatggaatt tttcaatttg ttcaacctga attactagga gcaagtgttt taaaagattt
     1801 aattaaaaag tatgaaattg ataaaattga tgagattata tgtggaaatg cagttgggac
     1861 aggagggaat atagctagat taatgacttt aacagcagga gtgtcaaatg aagtacctgc
     1921 ttttactgtg gatatgcagt gtgcttctgc tatgatgagt atagacatag ctttttcaaa
     1981 ggttaaatct ggtcagtgtg atttaattat tgcaggtggt tttgaaagta gttctctaca
     2041 accaatgcgc acatatcata agaatgataa gcgatataat acaaataatc caaattatac
     2101 agttgctcaa ttttctcctg atgataatag ccaaaatagt atgctcgaag gtgcggaaag
     2161 agtagcagag ttatatcaaa ttgagaaagc agacttggat ttttgggtaa aggaaagtca
     2221 taaaagagca aaagaagcaa gagaggaaaa aatacttgaa gatattatat ctcctataaa
     2281 taatagtacc tatgatgaag gtatacgtga taagatgagt caaaggttat tagataggat
     2341 gccatcaatt ttaggaaaag aaactattac aaatgcagca aattcatgtc ttattaatga
     2401 tggagcttca tttattataa tatgttctaa gaagtattta gaacatgtaa aaaagaagcc
     2461 taaagcaaaa attattaata catgcacaat aggaacagat gcaaccttaa gtccaacatc
     2521 agcgatacaa gcaatggata aacttttaga gatagaaagt cttaattatt tagatgtatc
     2581 tgctgttgaa tttaatgaag catttgcagt tatagatgtg ttgtttcaaa gaaaatatcc
     2641 agagttaata gatagatata atatatttgg tggagcttta gcgtatggac atccatatgg
     2701 tgcttcaggt gcaattattg cattacatct tttaaaagca ttagaaaaga ctaagggtag
     2761 atatggaatt tgctctatag ctgcagctgg tggtctaggt tcagctttac tgcttgaaag
     2821 ggtgtagatt atgaactatt atgaactttt aagaataaaa aaagagaggc ataaaaataa
     2881 aaatttttta attatagatg gagaaaaata tacttatgaa aatatattga gttatagtga
     2941 ggaactagga aaacaaattt ctttaggtga aaatatccca attttaatat attctaaaaa
     3001 tataatgttt cagttgatta gtttttttgc tattaattat tcaaaaaata ttcctattat
     3061 ttgtcattat aatctttcta aaaaagtatt agatgatatt ttattaaaaa ataatatttc
     3121 aatcattata tctgatgaat ctatggacgt tattgattta tatgatgata acaataatga
     3181 agtagcaaaa aaagttaatt tttctatttt taatcctaaa tttaatatat atatgtatca
     3241 atataagaac cttataaatt atttagataa aagtatttgt atgggagctt tatcttcagg
     3301 ttctacaagt gttccaaagg tattatatag aacatatgag agttgggctg gattttttcc
     3361 tatacaaaat ggtattttta atatctctgg ggaatcaatc ttgtttgtta atggaacact
     3421 tagttttaca gggaatctaa attctataat gtcagtatta tatgaaggag gtagtattgt
     3481 aataagttca ggtcttaatt gtaaatcatg gataaggata ataaaacaat ataatgtaac
     3541 taacatatac cttataccta caaaacttca acttttggtt aaacacttaa aagaaccagt
     3601 actcaaagtt gtaagtattt ttacagggtc tcaattatta tttgaagata ctgcaagaaa
     3661 tctaaaaaaa tatatgccaa agtctgaaat tattttatac tatggggcaa gtgaattaaa
     3721 ttatattaca tatttatgct atgatgaact aattgaaaaa cctctaagtg taggcagacc
     3781 atttccagga atagatatat atattagaga aggaaaaatt tttgtgaata cagaatatgc
     3841 agtatataat gcaaccaaac cttattctgt caatgatatt ggatattatg ataatgatgg
     3901 atacttaata tttgaaggaa gaagtgatga cattttaaat attggaggat ttaaagttag
     3961 ttctactaag attgaaaatg aagttaaaaa aattcctcaa attgaagatg caattgtatt
     4021 gccgtactca gacactataa gaggaaatca aattgtatta tttgtaataa ctatagacaa
     4081 aattacgaag aaagatttgt tgattaaaat gaaagataat ctaatgaaaa atgaaatccc
     4141 aaagaaaatt atctttttaa attcatttcc atatacctct tctgaaaaga tagatagatt
     4201 agcattatta aaaatgttat aagtataata tttagtaatt ttagtgaacc aatgtattga
     4261 taaaaacata ttgtatatag taaaggaggc ttacaatatg tcaaatcaat acgatataac
     4321 tgttattggg ggagacctgc gacaagtgta catggtaaaa aaactaatta ataaagggta
     4381 ttctgtagtc acttatggga tatcaaatga aattataaat ggaatcgttg gaaaatcaac
     4441 ttcactagct gaagcactat ccaaaagttc cattatttta tgtccaattc catttacaaa
     4501 gaatcaagtt aatattgaaa atacggatga ttgtcttgat gctactattg ataacttgct
     4561 aattaattta tctcctaatc aaatattatt tggaggtaac ataccaacta aagtttgtaa
     4621 attttgcgag caacactcta ttcagattta tgattttatg aaaatgaatg atgttgctat
     4681 tttaaatgca gttgctacag ctgaaggggc tatagcagaa gctattaaaa gaagtataat
     4741 caatatacat caaagtaact gtttagtatt aggttatgga agatgtgcac aaatacttgc
     4801 aaaaaaatta tacgctttgg atgcaaatgt atgtgttggc gcaagaaata atgatgcaag
     4861 aagtacggct aatgcatttg gatattccag tatttcttta agtcaacttg atgaaaaact
     4921 tttacaattt gattatattt ttaacaccat accagagtgt attttaacga aagaaaggtt
     4981 agaaaaagta tcacaagaag taactattat agatatagct tcttctcctg gaggagtaga
     5041 ttattctgtt gttaaagaag cagggattaa tgcgactctt tgccttgggc ttcctggtaa
     5101 atattcacca aagacatctg gtgaaattct tgttaacgct atagaaaata ttataaatga
     5161 aagaagggat tagaaatgag acttgaagga cttacaattg gagtaggatt tactggttcg
     5221 ttttgtactt atgataaaat atttatagag ttagagaatt tggttaaaga aggagctaat
     5281 gtacacacta tattttcaga tgtatcacaa aatatcgatt gtagatttgg aaattctgaa
     5341 gagtttatga aaaaagcata tgaacttaca ggaaataaac ctatagttac gattgaagag
     5401 gcagagccat ttggacctaa aggaattgct gatattataa taatagctcc ttgtactgga
     5461 aatactgctg caaaattagc aaatggtatt acagattcac cagtattaat ggcagcaaaa
     5521 ggtcatcttc gtaatgataa accactagta atatcaatat caacgaatga tgctctaagc
     5581 tttaatttca aaaatattgg cattctcctt aattctaaaa atatatattt tgtacctttt
     5641 ggtcaagata attgcaaagc aaagcctaat tctatgattg cacatactga gctaataatt
     5701 ccaactatag aacttgctct agagaacaaa cagctacagc ctgttataaa aagtccacat
     5761 caatagaatt ggttcttata atattatgta ttagatacaa tactagcaaa gaggctgtct
     5821 caaaatagag agaagcctct ttttcttgtt gaaatgatga aaatatattt aatttcatac
     5881 aaaaaaatgc ctcatggact aaaaagtcta ttttgagcta atctccgtaa tttttgctat
     5941 attaaaatta tttacttaat ccaattttta aattagaatt tatatcgttt tgactcttca
     6001 aatatcaatc taactgttgt tgatacaaaa gaagaggaca taattaaaac aaatactgat
     6061 agtcctctaa taaacaaatt atctacataa taagttacta ttccaatacc tccaatcgtt
     6121 aaaattacac ttataatcaa agaaaaaacg ctaattgttt ttaatttttc catgcttatc
     6181 ctccttctta taaacttcaa atttatctat ataatattaa atatatttta tttcatatta
     6241 tataaaaatc atgaatatat atttttttaa ttatactata tgttgtaaat gtttgtacat
     6301 ttaactatat ttactttaac ttgttaatta agttttaatt ttcattttca aggattttta
     6361 tttttaaact tgatagagta tcatattaaa aattcttttt ataaagtttt atttgcaaaa
     6421 aatacaaaaa tataaacgtt aaaaaaataa cgttatcctt aagtttttag atgatttcat
     6481 tagtgatttg cgtaaaaaat aaagtgatat ctagttaaat taagtatttg taaagctaga
     6541 tattattagt agaaatgatg ttatattaga taaaaaataa aatgttaaaa aaataacttg
     6601 aaaataggaa aagtaaatgc tataatgtta ataaattaac ataataaata aaaaactatc
     6661 tcaggaggtt gttaaaatgg ctcataaaga acaagtaata atagaaaata aaaaagaaaa
     6721 tgaagttatg tcagttggtt tagatactgt aaataacgtt gaaagcttag ttaaaaaact
     6781 agcaaaaatc agagaagctc aaaaaatatt tgctacatat agtcaagagc aggtagataa
     6841 aattttttta gcagcatctt tggcagctaa taagcaaaga gttcctctag ctataatggc
     6901 acaaaaagaa actggtatgg gaattgctga agataaggtt ataaagaatc attatgcctc
     6961 agagtatata tataatgcat ataaagaaac taaaacttgt ggtgttatag agaaagacga
     7021 agcatttggt atgactaaaa ttgctgaacc aataggtgtt attgcagcag ttgtacctac
     7081 aactaatcca acttctactg ctatctttaa agcactttta gctttaaaaa ctagaaatgg
     7141 aataattttc tctccacatc caagagcaaa aaattctact atagaggctg caaaagttgt
     7201 tcttgaggct gctgttttag ctggagctcc tgaaggtata ataggatgga tagatgagcc
     7261 atctttagaa ttaactacaa ctgttatgaa agaagtggat ttgacccttg ctacaggagg
     7321 tcctggaatg gttaaatcag catattcatc tggaaaacca gctattggag ttggtgcagg
     7381 taatactcca gcaataattg atgatagtgc agatataaaa actgctgtaa actcaattct
     7441 tgtatctaag acttttgaca atggtatgat atgtgcttct gaacaatcgg ttatagtatt
     7501 agaaaatata tacaatgaag tcaaaaaaga atttaaagag cgtggtgctt atttattaga
     7561 taaagatgaa acagaaaaaa taagaaatat aattttagta aatggaggat taaattctaa
     7621 aatagttggg caaacagctt gtactatagc aaaacttgca ggctttgaag tcccagtaga
     7681 tactaaggtt ttaataggtg aagtagaatc agttgaaata gaagaagctt ttgctcatga
     7741 gaaactttca ccagtacttg caatgtacaa ggcaagtgat tttgatgatg cagttagaaa
     7801 agcagaaaaa ttagtagaag atggtggatt tgggcataca tcttcattat atattgatga
     7861 tgtaaatcaa agagaaaaac ttgataagtt tacttcagcc atgaaaactt gtagaatttt
     7921 aattaatact ccttcttctc aaggtggtat aggggactta tataatttta aactagctcc
     7981 ttctcttact ttaggatgtg ggtcttgggg tggaaattca gtatctgaaa atgtaggagt
     8041 taagcattta cttaatatta aaacagtagc tgagaggaga gaaaatatgc tttggtttag
     8101 agcaccagaa aaagtttatt tcaaaaaagg ttgtttagga gtagcattaa aagaacttaa
     8161 agatgtaatg aataagaaga gagcttttat tgtaacagat acattccttt acaataatgg
     8221 atatactaaa gcagttactg atttattaga tgaaatgaat attaagtaca ctacattctt
     8281 tgaagtagaa ccagacccaa ctttggaatg tgctaagata ggcgctaaag ctatgagaga
     8341 atttaatcca gatgtaataa tatctatagg tggaggaagt gcaatggatg caggaaagat
     8401 aatgtggaca ttgtatgagc atccagatgt agatttccaa gacctagcta tgagatttat
     8461 ggatataaga aaaagggtat atacattccc taaaatgggt gaaaaagcta attttgtagc
     8521 tataccaaca tcagcaggta ctggttcaga agtaacacca tttgctgtta taactgacca
     8581 agatacagga gtaaaatatc cattagcaga ttatgaactg atgccaaata tggctatagt
     8641 tgattctgat atgatgatga atatgccaaa aagcttaaca tctgcttctg gtatagatgc
     8701 tttaactcat gcattagaag catatgtatc tatgcttgca accgattata caaatggatt
     8761 agctttacaa gctattaaga gtatatttga atatcttcca cgtgcatatg ataatggtgc
     8821 aaaagaccca gaagctagag aaaaaatggc aaatgcttct acaatggcag gtatggcttt
     8881 tgcaaatgca tttttgggtg tttgtcactc tatggctcat aaattaggtg ctttccacca
     8941 tgtaccacat ggtgttgcaa atgctctttt gataacagaa gttatgaaat ttaattcttc
     9001 agatgcacca aagaaaatgg gagcattctc tcaatataaa tatccagaag cattaaagag
     9061 atatgctgaa atagcttcat ttttaggatt aaaaggaaat tctgatgaag agaaattcca
     9121 aagtttattg gtagctattg aagatttaaa acttaaagtt ggtattccta aatctataaa
     9181 agagtttggc gttgaagaat ctaagtttat ggattcaata gatgaaatgg taattcaagc
     9241 atttgatgac caatgtacag gagcaaatcc aagatatcca cttatgaatg aaataaaaga
     9301 tatgtattta aattcatatt atggaagata agtttgctat aaaatagata aaataaaaac
     9361 tcttatacat aaaacaaagt tttattgtat aagagttttt ttgtttgaga aaatggatag
     9421 tttttattct ttaatttaaa attcaattaa atagtgttat tataataata aaagaatgtt
     9481 tttatggtta ttatgttgat tttatgtgta acatttaaga atagttatat acaatataaa
     9541 tgatgaattg gtataaaaag gatatattat ctatatgaag tattgactaa ttaggggagg
     9601 agtcgaatga cagggagaaa taaaagtttt acattaatta caattctttt tttaacatca
     9661 ttatttttga caatggggta tatctatata gaagatacaa aacatcttct tatgtcagag
     9721 gcaaaaatac atataaaaga agttgcagta caaggctctc aactagcaca aagacaaatt
     9781 gaaaaagatt tagatgtttt aaatattttt tctaactatt atgcttcaaa tccagatata
     9841 cctaatgagg aaaaaatgaa aaatctttta gatgaaatgg aaaatcaaaa attttacact
     9901 atggcaatag ttgatataaa tggtaatgct gaaaatacca aaggtgataa gttttcagtg
     9961 aaagatagag aatttttcaa aaactcaatt aaaggtaaga agtatgtttc aagtccatat
    10021 gttgatgaag taaataagtc tataaagaag attgctattt cagtaccatt attaaataat
    10081 gataaagtag ttggagtttt atattgtact tacaatatca atactctgat gaaacttatt
    10141 aatatatctt tttatgaaaa taatagtata tcttatgttg taaaaaatga tggtacaatt
    10201 atccttcatc cacaagggga tagcctatca aaaaatattt ataaattgtt agaacaggat
    10261 aatgatattc aagaagtaaa tagattaaaa aaagaattgc aagaaaataa aacaggtgca
    10321 actgtattaa atatgttgga agaaagaaga tatttagggt atgccactat ggataatggg
    10381 aatagtgaaa ataattatat aaaagactgg aatttaattt tttcaattcc agaaactgtt
    10441 gtatttagta attcaaaaca aataatcaac agagctgtat atgcagtgtt atctattgta
    10501 ttaatatttg ttgcaattat tttttatata atttatataa aaaaatctaa tgaaaaagaa
    10561 atattaagtt tggcttatga agataaagtt acatatatag gaaatcaaaa taaattttat
    10621 agagaatgta gtaagtatct tttagataaa ccatctttaa attatattat agtatatttt
    10681 gatattaata actttaagat gattaatgat acttttggat atgaatttgg tgacaattta
    10741 ttaattacaa tagcaaaagc tttaaaagaa gaattaacag aaggtgaggt atatgcacga
    10801 ctttcaagtg attattttgc aattttctgt gattataaaa atggaagaaa taaaattata
    10861 agaaaacttg ataatatacg aagtaatatt gaaagtaatt tgagtatagt ctttgaaatt
    10921 tctctgtgtg tgggtattta ttttgttgaa gaaggtgaag tagatattca aaaggctgta
    10981 aataaagcaa atatggctag gtctgttgct aaaggtaaaa atataaatta tgctatttat
    11041 aatgaagatg ttaggaataa gcttagtgag gaatcaatga ttttagacga tataaaaata
    11101 gcattggtaa aaaatcaatt tgaagtgtat tatcaaccta agttttcact tgtaactgga
    11161 gaaatgatag ggagtgaagc actaataaga tggaatcatc cagaacatgg ttttataagt
    11221 cccgcagtat ttattccaat agcagagaaa agtaagttga ttttaaaaat tgggagattt
    11281 gtatttgaaa gggtttgcaa tgatttatat gaatggaaaa agcaaggtaa aaagatagtt
    11341 ccagtttcag taaatttatc tagggtcgag ttgtatcaac cagatattgt aaaatttatc
    11401 aataaaacaa taaaaatgta taatttaagt tcagatttca ttgaaataga gataacagaa
    11461 actgtggcta ttaatgagtt aaatatttta aagaatgttt taaatgagtt gaggacgtat
    11521 ggattttcta tttctatgga tgactttggt acaggatatt cttctattag ttgtttaaga
    11581 gatatgccaa ttgatatatt gaagctagat aagtcttttc ttggtggcat tgaacatgat
    11641 gagagaagtc gcaatatagc aaaatcaata gtgtctctag caaaatcact agatttagtt
    11701 gtaattatag aaggtgttga gagtaaagaa caagctgagt taatgaaaca atttggatgt
    11761 gatttagttc aaggatttta ttttgcacga ccaatgccag ctaagaattt tttagattta
    11821 ctttaaaaca aaatttgatt tttatataaa gcactattat gaatgcttga ttttaataaa
    11881 tcattgctaa ataattagtg tttttttatt ttatcaatgt attgtctaat ataaagcaaa
    11941 aaataattgt attttataaa aattatatta aaaataaatt tattttgcta ataaacatgt
    12001 tattaacaaa agaatagtac tataatgtat agatgataaa aactttaata aaaatttagt
    12061 gtataggagt tgtttaagta tgatagatat aagtattgat gacaaactta aggaaagatg
    12121 gcctaatgct aagctgggtt gcattcaagc aaaagtagca gtaaataaaa gctcagaaaa
    12181 attaattaat gaaataaatg agttttgtga tactataaat caaagtctaa aagttgaaga
    12241 cattacgaaa ctagataaaa ttaaagatgc aagaaatgct tataaagagc ttggaaaaaa
    12301 tccaagtaga tatagaacat cttcagaagc attggttaga agaattattc aagggaaggg
    12361 tctttataca ataaataata tagtagaaat aaacaattta atatcaataa aatcattata
    12421 tccagtaggt acatataatg taagcaaatt acattcacca gtatgtttta ctgtaggaga
    12481 tgaaggagaa cagtataagg gtattgggaa agaattaata aatatagaaa atcttcctat
    12541 attgtctgat tcagtgggta aatttgggag tccaactagt gattctgaaa gagcaatgat
    12601 aacagaagat gcaaatgaaa tattaatttg tatattttct tttagtcaag aatctgatat
    12661 tgaagaatat tttggatatg ctaaggaatt acttaaagaa tatgcagatg gaaaagatat
    12721 agaaactaaa ataattaaat agaatctata taacaacgtt atgttaagct aaaattcaat
    12781 ataaaaataa gttaagataa agaaactaac acaaaattag tttctttttt aaaatagtag
    12841 tttagtgtta caaaaataga tagatagtcc cacgattatt aaaagtatgg taatatatta
    12901 atatagcaga gattgtgttt aagggatttt gaaagggggg atttataata atcattttat
    12961 ctaaaagata aaatggtttt atagaaataa ctatatattt aaaaatataa ataatgatac
    13021 agtaatttgg gtatataaaa taaggcatac atggttttgt ttaaaaacca ataaggtaca
    13081 taggtaatta caatgtaagg aaggatttaa ttatgattga tggaaaagag gtaaaacaag
    13141 aaagtggaca aaaaaatcta aaatttaaga tagctatagt agtaggtgtt ttgtttgtag
    13201 cttatatagg aatggcaatc ttttttagaa atcattttta ctttggaacg actattaatg
    13261 gcgtaaaggc ttcaggaaaa acagttgagc aagtaaatga tttgttagca tctagtacta
    13321 attcctatac tttaaactta gaggaaagaa acaataagaa agaacaaata aaagcaaaag
    13381 atattgattt aaaatttaca ccagatgata aagttcaaaa gataaaagat agtcagagcg
    13441 ctattggatg gattggtgga atatttaaat ctaaagagta taataatatg ataactcttt
    13501 catataataa agatttacta gaaaaaaggt ttaatgcttt atcttgtttt gatagtaaaa
    13561 atgttgtaga acctaagagt gcttatccta agtatgatag cactaaaaac gcttatgtta
    13621 tagtaccaga ggtacttgga aacaaagtga aaaaagatga tttttataag ttagtacaaa
    13681 actcaattaa ttctgggaat acttctattt ctctggatga aagtaaagtt tatgaagcac
    13741 caaacaacac aaaggattca gaaaagttga aacaagctat taagacatta gataagtatg
    13801 ttggtgtagt cgttacatat gattttagtg accgtaagga aactttagat ggttctgaaa
    13861 ttaataaatg gttaaaagta gactcagaaa agaattatga cataactttt gataatgatg
    13921 ctatggtaga atatacaaga ggtttaagta aaaaatacag tacttttggt gactcaagac
    13981 catttaaaac tgccacaggt aaagatatta caatttcagg tggaatttat ggttggttga
    14041 tagataaaaa gaaagaagct gaagcattag ttgatgttgt aaaagctggt aaaaatgtta
    14101 caagagaacc tatttacata caaaaagctg tatctcgtaa taaagatgat attggaaata
    14161 catatgtaga gattagtctt tctggccaac atatgtggtt ctttaaaaat ggagaaatgc
    14221 ttgtatcaac agacgttgta acaggtgatt tagctagagg atttgctaca ccatcaggta
    14281 tatatcctct taattataag gctagaaatg tttcattaac tggtcaaggt tacagttcac
    14341 ctgtaagtta ttggcttcca tttaatggga acataggcat tcatgatgct acttggagaa
    14401 attcttttgg tggaagtata tataagtctt caggttcaca tggatgtgta aatacacctt
    14461 tttctaaggc aaagaaaata tatgaaaata tagaaccagg tacaccaatt attttatact
    14521 agtaaatgtg ttgctaacag ttaagcgaag tcatcctagt ttatttagga tggcttcctt
    14581 tttatataaa gcttattgaa gtttacagaa agactactta aaaggagaaa tacataatga
    14641 atgaaaaatt aactaaaatt ttagaagaag ctaagaatga tgatgaattt agaaaggaac
    14701 ttataaaaac aagagaacaa cgtgatgtta tggattcatt ttgtaaggtt gttacatcta
    14761 gaggttatga tatgacagta ggggaacttt tttctatggg agaagaatat acatccaatc
    14821 tactgaaaag tgtaaatgga ggagcagcat atcctattga agattggagt gattggtacg
    14881 aggatttttt tataatgttg tcgaatatat agagtaacta atgatattat ctacttaaga
    14941 ttaatacatt gccttaaaat aaaactatta gaactaggtg taagttttat caatctttaa
    15001 aaatattgat ttatttctaa ctagatataa aaaaattaga ttaaaaataa ggtgacaaaa
    15061 atcgatatag cttgatgatt tataaaagtt agataaaata gtgcgagata tttctaaaaa
    15121 ttcaaaaaaa acaataaact tcaatggtta gaggttgaaa aagaatataa aaaactttta
    15181 tatgtaataa attaaattaa aattataaaa aaaataaaat aaaaatttaa aatgtcgaca
    15241 taacgttaat tttaaaacaa aataacgcta ttgacaaact atatcgcttt aaattataat
    15301 tattgaaaat aatggtattt aaaaaccata ctcatgtcat agtctaaaag taagtcaaaa
    15361 aaggatgtaa atctaggcaa gtactattct aaaatatgtt ttattaagaa ataaacgttg
    15421 aaaaatatta attattatat aaaaatgctg tttatacttt aacaatgagg tagtaatcta
    15481 attagaataa aaaaatctaa ttggttatat acatagtaaa aaggtaagag aggtgatttg
    15541 gatggctttt gaaataataa aaagcattgt tgaggcagag cagacagcag acagtatcaa
    15601 agtaaaagct gttactgatg cagagtctat cagagctgat gctgtaaaca aatgtgaaag
    15661 catatttgct gatgtaaaaa aacaagcaaa gcttatggaa gaaactctta ttgagaaggc
    15721 agtcaccgac agtagagcag aggttgataa aatcttagct aatgctaaaa gtgaatgtct
    15781 gaaaattgaa aaaactgctg aagaaagaaa aagtaaggct attgaagcgg ttattggaaa
    15841 ggtagtgaga taatatggca atagttaata tgaacaaaat atctattgtt ggcttagaag
    15901 ctcaaaaaag ccagatttta aagctactta tgaaccgtgg gtttgttcag attgatgata
    15961 gtgcctttct tactgaggag gaggaattaa aaaattatct tgtaaaagat agtgaagaat
    16021 ctgaagttat aatgctagag cagaaaatgt ctcatgtaaa ccaagcaatt aatattgttt
    16081 cttcactagt gaaacaaaaa aaatccctac ttgcaccaaa aagagagttt aacaaaattg
    16141 atagtgaaaa agaagggcaa gtttatgctg aagctctgga gttaaacaat ttgcacaagg
    16201 agataggtac aataaggtct aacataaact tacttaacaa caataaaagt atgttagaac
    16261 catgggtgaa ctttgatatt cctattgaaa atatggaaac taagtataca aaaactatac
    16321 ttggaactgt gcccataagc gcaaacattg atgctttaag agtaaaatta gaagatgaaa
    16381 taccagaaag tgttattggg attgtcaatt ctgacaagtt aatgaactat gtttatttaa
    16441 ttactttaaa agaaactttt gatgatgttt tagaagtttt aaaagaattt aatttttcag
    16501 tggtttcatt accaaatgaa gaaggtacac cgttacaagc tatcaagaaa tacaatgaaa
    16561 tgataattag acaggaaaac gtttctaagg acaaaacaga ggaaattaaa agacatgcag
    16621 atagtataca catgcttgaa aacctatatg atttttacac tatagaaaga gaccaaaaaa
    16681 agattataga gagacttgtc aaaacaaaaa caacattctg tcttaatggt tggttaccaa
    16741 gcaaaagggc agatgaatta attaaagaaa ttacagaaaa gtttgattgt tgtgttcaga
    16801 ctgaagaagg aagtaaagaa gaaggcttcc caattctgct tgaaaacaat aaattagtaa
    16861 caccttttga aagtgtaact aacatgtaca gctatccaag tacaaaagat attgacccaa
    16921 atacaatttt aactttcttc tttgttatat tctttggaat gatgctaagt gacgccgcat
    16981 atgggttaat aattgcagta gtttgtggat ttgttgtata taaattaaag atacaaaaag
    17041 gtgaaggaaa tttaattaaa cttattggta tctgtggagt ttcaactact gtatggggtc
    17101 ttatattcgg gggtattttg ggggatttaa ttcctataaa agcattaatt aatcctttag
    17161 aggatgttat gacacttatg ggaatgtcgt tactgttcgg tattatccat atatatgtag
    17221 gtttgggaat aaaagcatat acattaataa gaagtggaaa ggtaatagat gcaatctttg
    17281 acataggttt ttggtatttg tgtataacag gtgtatgtct acttataata ccttttgttg
    17341 caggggacat aggtgtattt tcagaagtag gaaaatattt agcaattata ggtgctatag
    17401 gtcttgtact tactcaagga agaagcttta aaggtattcc tgttaagctt tttaaaggat
    17461 tttcaagtct ctatggtata acaagttatt ttgctgatat attatcttat acaagaatta
    17521 tggcgctttg tttaacaaca ggagttatag cacaagttat caacctttta ggggctatag
    17581 ctggtccgat acttgcagtt gtaattggtg ttgtaggaca cacaataaac cttcttataa
    17641 atgcactagg tgcatatgtt catacaagta ggttacagta tgtagaattt ttcaataagt
    17701 tttatgaagg cggaggagtt ccttttgttc cgtttaagta taaaacaaaa tatacaagca
    17761 ttaataaaaa ggagatgtaa atatggaatt tttaaacgcg ttaggaaatg gtcaattttt
    17821 ggcaatatca ggagctgcta tagcagcatt atttgcaggt atgggttctg ctaaaggtac
    17881 ttcaatagca ggtcaagccg cagcaggtgt tgtaacagaa gaccctagta aattcggtca
    17941 acttctagtt ttacaacttc ttcctggtac acaaggtctg tatggattta tcatagcttt
    18001 ccttatttta tcaaaagcag ggattattgg tggagatgca ataccaacat cagctcaagg
    18061 tttacaactt cttatggcag gacttccaat tggtttagtt ggtttagtat caggtatagc
    18121 acaaggtaaa gcagcagcag caggagttgg aatagttgca aaaagacctg aagaacttgg
    18181 taaagctata acatttgcag caatagttga aacatatgct cttttaggac tacttgtttc
    18241 tttcttagct tacaatggaa ttgcaattgg ttaattataa attataattc ataagtaaaa
    18301 aaaatgaaaa ggaggaagca ctcctatggg taatgaacaa aaaatgattg acagaattat
    18361 agctgatgcc aaacaagaag cacaagaaat attggataaa gctaaaagtg aagcagattt
    18421 aaaagtaaat tcagctaatg aaaaagctga aaaggaaatg gcttcctata caaagcttgc
    18481 agaggcagaa gcagaaaaag cggcttctaa ggaaatatca ggagcttata tggaggctaa
    18541 aaagcaaata ttatcaaaaa aacaagagat tttagaagag gtaattttag aagctaaaaa
    18601 caaactttta aatcttaaag ataatgaata tgaagagatt atactaaaca tgattgaaaa
    18661 atcaaattgt acagatgatt cagagatagt attatctaaa aaagataaaa aaacattaaa
    18721 agatgtgcta tcaaaaaaag gcattaaagt gtctgatgaa actagagata ttactggagg
    18781 tttcatagtt aaaaaaggtg acattgagta caattattca tttgaagcca taattgcagt
    18841 agagcatgaa tatattgaac aaattgcagc agaaattttg tttaattaag gaggggttaa
    18901 aaaatggcag aagaaaaaac ttatccctat gccgttgcac gaataagagt gcttgaaaaa
    18961 cagcttctta acagacaaat gtttattcaa atggcagaag ccaaaagccc tgaagactct
    19021 ttaagaatta tagctgaggc aggttatgct gatacatcaa ataataatat acataatttt
    19081 gagaatattt tgactcaaga attatcaaag acatatgaaa ttcttaaaag tctagctcct
    19141 gaagaaaaat ttgttgatgt atttctttat aaaaatgact atcacaattt aaaagtgctg
    19201 attaaagaag aaatttctgg tgtagatggt gagaaatatt taattgatgg aggtacaatt
    19261 cctcttatta agcttagaga atctttagca aatcgtagtt ttagtgattt acctaagata
    19321 atgtcatcag ctatattaga ggcatttgat gtttataata agacacaaaa tggccagatg
    19381 gttgatattg ttctagataa agcaaccttt accagtatga aagaaactgc taaagaaagc
    19441 caaaacaaat ttgtaataga ttatgttatg aaagtttgtg atttaacaaa tctaaagagt
    19501 tttataagag tcaaaaatat gaagaaagat tttgatatgt ttatgaatgt gtttgtttca
    19561 ggtggctcac ttgataagga aaaattctta gaagcttttt cctcagatac tccagcatca
    19621 tgttttaaat ctacttctta ctctgatgtc tgtaaaaatg gcatggatag tggttttact
    19681 gtctttgaaa aactttgtga tgactatctt atggaatatg ttagaggagc aaaatttaag
    19741 cctcttacat tagaaccact aattgcatat ttatatgcta aagaatcaga gataaaaaca
    19801 attagaatta ttttgactag taagcttaat aatatagacg cagatactat aaaagaaagg
    19861 ctaagggatg cttatgtata aggtaggagt agttggagat aaagatagca tcatgggatt
    19921 tttagctctt ggaatagata tcttcccagc ctatgatagt gacgaaatta aaaaaagtat
    19981 acataagttg gttgaagatg aatatgcgat aatttatatt acagaacagg catcattgct
    20041 tgcaaaagaa tcaatagcaa aatacaaaga ttaccaactt cctgccatta tagttattcc
    20101 aggtattgga ggaagtatgg gattaggtat gaatgaagta agagaatcta gtaaacgtgc
    20161 tataggtgca gatatattat ttaaagaata gatgaaagtt gaataaaaga caatctcaaa
    20221 ttttattggt gctaaatata tgtgctaata aaatatagga gggaatattt aaattttgat
    20281 aaagtatatg attgcaggtg agagaatatg aaaacaggca caattgtaaa ggtgtcagga
    20341 ccgcttgttg tagcggaggg catgagagat gcgaatatgt ttgacgtcgt aagagtttca
    20401 gataaacatc ttataggcga gattatagaa atgcatggag ataaagcttc tatacaggtt
    20461 tatgaggaaa cttccggtct aggaccaggg gaagaagtag tttctcttgg aatgcctatg
    20521 agtgttgaac ttggaccagg tcttatttca actatttatg atggtattca acgtccactt
    20581 gaaaaaatgt atgatatttc aggtactaat ataactaaag gtgttgaagt atcttcactt
    20641 gatagaacaa ctaagtggga atttaaacct aaaaaatctg ttggtgataa ggttgtagca
    20701 ggagatataa ttggtggcgt tcaagaaaca gcagtagtag aatgtaaaat tatggttcct
    20761 tatggagtta agggtactat aaaagaaata tattctggtg aatttactgt tgaagataca
    20821 gtttgtgtta ttacagatga aaaaggtaat gatatacctg taactatgat gcaaaaatgg
    20881 ccagttagaa aagaaagacc atatagagaa aagaaaacac cagattcact tcttgttaca
    20941 ggtcaaagag ttatagatac atttttccca atcacaaaag gtggggtagc agctataccg
    21001 ggaccttttg gaagtggaaa aactgttaca cagcatcagc ttgctaaatg ggcagatgca
    21061 gatatagttg tatatatagg ctgtggcgag cgtggaaatg aaatgacaga tgttcttctt
    21121 gaatttcctg aattaaaaga cccaagaaca ggcgagtcac ttatgcaaag aactgtgctt
    21181 atagcaaaca catcagatat gccggttgct gcacgtgaag cttctatata cactggtatt
    21241 acaatagctg aatattttag agatatggga tatagtgttg cacttatggc agactctaca
    21301 tcaagatggg ctgaggctct tagagagatg agtggtcgtt tagaggagat gcctggtgaa
    21361 gaaggttatc ctgcatactt aggttcacgt cttgctcaat tctatgagag agcaggaaag
    21421 gtaaattgtc taggtatgga tgaaagagaa ggaacactta cagcaattgg tgcagtttct
    21481 cctcctggtg gtgatacatc agaacctgtc agccaagcaa cgcttcgtat agtaaaagta
    21541 ttctggggtc ttgatgcatc tttagcatat aaacgtcatt tcccagcaat taactggttg
    21601 actagttatt cactctatca ggaaaaagtt gatgtatggg cagataaaaa tgtaaatgat
    21661 aatttctcag ctcaaagagc tcaagctatg aaacttttac aagttgaatc tgaacttcaa
    21721 gaaatagttc aattggttgg ggttgattca ctttcagatg gagatagatt gatactagag
    21781 gttactcgtt ctataagaga agacttcctt catcaaaact ctttccatga agttgatact
    21841 tatacatcac ttcataaaca atatagaatg atgggactta tattaaaatt ctataagtca
    21901 gcacaagatg ctatcaagca aaatgttact ataaacgata ttatcaagct ttcagttctt
    21961 gaaaagattg gtcgtgctaa gtatgtagaa gaaggcgata tagatgcagc ttacgattca
    22021 attgaagatg aaatagacaa cgagcttgca gaccttatta aaaagagggg ggctgaagaa
    22081 ctatgataaa ggaatataag tcaatacaag aggtagccgg tcctcttatg ttaataaacg
    22141 gcgtagaagg tgtaaaattt gatgaacttg gagaaattga actttcaaat ggagaaattc
    22201 gtcgttgcaa agttctagag gtaaatggag atactgcact tgttcagctt ttcgaaagct
    22261 caacaggtat aaaccttgct gaaagtaaag ttcgtttttt aggaaagagt cttgactttg
    22321 gagtttcacc agatatatta ggtcgtgttt ttagtggaat gggtagacct attgatggag
    22381 gtccagaaat tataccagat aaaagacttg atataaatgg agctccaata aatccagcag
    22441 cacgtgatta tccggccgag tttatacaaa ctggagtctc tgctatagat ggacttaata
    22501 cattggttag agggcaaaaa cttcctatat tctcagggtc tggtttgcca catgccaatc
    22561 ttgcagttca gattgcaaga caggcaaaag tacgtggaac tgattctaag ttcgccgttg
    22621 tatttgctgc agttggtata acttttgaag atgctgaatt ttttataagc gattttaaag
    22681 caactggtgc aatcgaccgt tctgtagtgt ttgtaaacct tgcaaatgac ccagctgtag
    22741 agcgtgtatc tacacctaga atggcactta cagcagcaga ataccttgct ttcgagcaag
    22801 atatgcatgt acttgttata ataacggata ttacaaacta tgcagaggca ttgcgtgaag
    22861 tttcagcagc taaaaaagaa gttccaggac gtcgtggata tccaggatat ctttatactg
    22921 accttgctac tatgtatgaa agagcaggaa aaatgaaagg gcatgaaggg tctataacta
    22981 tgattccaat acttacaatg cctgaggatg ataagacaca tccaattcct gaccttacag
    23041 gatacattac agaagggcaa attatactta gccgtgaact ttataaaaaa gatatacagc
    23101 ctcctataga tgttttacca tcactttctc gtctaaaaga taaaggtatt ggtaaaggta
    23161 agactcgtga agaccatgca gatacaatga accagctttt tgcagcttat tcaagaggta
    23221 aggatgcaaa agaacttatg gctatacttg gtgaatctgc actttctgag attgatttaa
    23281 tgtatgctaa gtttgcagat gaatttgaga aagaatatgt ttcacaagga tttagaactg
    23341 atagaactat tgagcagaca ttagaaatcg gatggaagct tcttagtatg ttaccaaaaa
    23401 gtgaacttaa acgtatccgt gatgaatatt ttgaaaaata tatgcctaag gagtgatatc
    23461 tgtggctaga ttaaatataa atccaacacg tatggaaatg actagactta agaaactcct
    23521 taaaactgct acaagaggtc ataagcttct taaagataaa cttgatgaac ttatgaaaca
    23581 atttcttgag attgtaagag aaaataagcg attgcgtgaa gaagcagaaa atgctcttga
    23641 tactgcttat aaaaacttta taatcgcaag agcagttatg agtcaggagt atctgggttc
    23701 agcacttatg atgccaaagc aatcagtttc agtagatgtt tctacgagaa atataatgag
    23761 tgttgatgtt cctgtgtttg actttaaaac tgaaaacaat caaagtgata tttatcctta
    23821 tggtcttgct tttacatcag gtgaattaga ttctgctatg gaagctttct cagatgctat
    23881 gcaaccactt ttaagacttg cagaaagtga aaaaagtgca cagcttcttg cacaagagat
    23941 agaaaagaca cgtagacgtg ttaatgctct tgaaaatgta atgataccaa attacattga
    24001 gactattaag tatatagcta tgaagcttga agaaaatgag agagcaagta ctactagact
    24061 tatgaaggtt aaggatatgg tgcttaaaaa agcacttgaa gaaaagaaaa aaaatgattt
    24121 ggttgtataa ttaaatactt ataaaaataa gtctaacatt ctactttata gaggttagac
    24181 ttatttttta tgtttaaatt aaaatgtaga tactataaaa attaatatat tgtttaaaaa
    24241 cagactgtat ttaactaaaa ataatatatt taacaaaaat ttctaaaaat taacgaactg
    24301 ttaatttaca cttaacttta tattaacaaa ttaggtgtat tatataaaca taagcaaaaa
    24361 caataaaaaa caaataaaaa aaggaaaaaa cacaaagggg gaagatatga aagaatttat
    24421 atttacttgt ctgtgcattg tagtgattac atttatgaca agttgcatat caacaacacc
    24481 acaaactact caaggaaaag gaaataataa acttacaata tatgcagcac tagaggaaga
    24541 gcagatagct tattatatgg aaggttttag agaagagtat cccaatatag atgtagagat
    24601 agttatggat tctcatggtg taatagcttc aaaactatta gcagaaaaag ataatccaca
    24661 agcagatatt atatggggat tatcagcaat gaacatgata gagctaaaaa aagatggtgc
    24721 acttgaagca tataagccta aaaattatga taaagttgat aaaaaattta aagatactga
    24781 agatgatgta tcttgggtag gaatgagtgt cccagagaca gcaatagtag ttaattataa
    24841 ggaacttaag aaactaggta tagatattcc aaaaagttat gaagatttaa taaaaccaga
    24901 atataaagga cttataacaa tgccaaatcc agcatcatct ggaacggggt atcttacagt
    24961 atcagggctt attcaaatga tgggtgaaaa agatgcttac aattacatgg ataaattaca
    25021 taaaaatata gccacatata cacactcggg ttcaaaacct gcgaagttag ctggctctgg
    25081 agagtatcca ataggtatta cacttggata tagagctaca caacaattat ctagtggaga
    25141 acctatagag gttgtatttc caaaggaagg ttcaggttgg gacttagaag caaatgcctt
    25201 agttaaaaaa gataatataa aaaaagaagc aaagctattt ttagattggg caataagtga
    25261 ttcagctatg gatagatatg cacatgaagt agcagttact tccataaaga cagataatcc
    25321 agttccaaaa ggttattcta aacaaccatt tgaacagctt ataaaagaag ttgatttaaa
    25381 ctctgctgcc aaaaacagaa gtacagtact taaaacttgg gaatcaaaat acggttcaaa
    25441 atctgaaagt aagtaaagtt taaattaata atttagatga aagaatttta gaagaaaaag
    25501 taggatataa aatttaaaat aaaaaaataa ctatataagg agagataaga tatgagttac
    25561 ttaaaaataa ataatgtgtt taaaagttat gaccaaaaaa aggttttaaa taatattagt
    25621 ttggatattg aagaagggga atttctatgt ttactcggac ctagtggatg tggtaaaact
    25681 acattactta gaataatagc aggtcttgaa gatgtaaata gtggaactat aattctccag
    25741 gataaagata ttacaaattt agagccatca aagagaggtt ttggtatagt atttcaatca
    25801 tatgcattat ttccgaatat gactgcttat aacaatatag cttttccact aaaagagaga
    25861 aaagtttcaa aagaaaaaat agataataaa gttaaggaag tacttgaaac agttggattg
    25921 actaatgaag ctcataaata ccctaaagca ttatcaggtg gacaacaaca gaggattgca
    25981 atagcaagag cattagctct tgaaccaaag tttttacttt tagatgaacc aatgtctgca
    26041 ttagatgcaa aggttagaca caagcttaga atggacatta aaagattaca aaaagaactt
    26101 aatataacga caataatggt aacacatgac caagaagaag ctataactat ggcagataaa
    26161 atagctattc taaatggagg agatataatg caaataggga ctccagaaga aatataccaa
    26221 aatcctcaaa acctatttac agctcaattt ataggagata caaactgttt tgataatgga
    26281 gattctatat taactgtaag accagaatat gttcaaatag agaaatcgac aaaagaaaac
    26341 taccaaggga taatatcaaa tatagagttt agagggaatt tattgagagt tgaaataaaa
    26401 gataaattaa atgaaaattt cataataagt gatgtttcta taaaagagtg ggttaattta
    26461 aatttagtag aaggtgacct tgtaaaaata agtatagacg aaaaatacta tttaaagtat
    26521 ccaaaagtta aaggagcata agtgtatgaa taataatgct ataagttcta aaagtataag
    26581 tcttaaatca aaaatgaatt tagatataaa aaaatgtatg cttacagtat tagttatgtc
    26641 tttagtaata tttttattag cacctataat atatctattt gtacaggctt ttttagataa
    26701 agatggagtc tttgtaggat tacaaaactt tcaagaatac ttggcttctg aatctctatt
    26761 aaattctcta aaaaatacag tatttgtatc agttatatca actgtaatat cactaacact
    26821 tgcattagta tatgcatatg cattaactag aacaaagatt aaatttaaag gtttttttaa
    26881 atcattagct atgtttccta tgtttgctcc aacaatgatg catgcaatag gtctagtata
    26941 tttatttgga aatcaaggtt tggtaactaa tttacttgga gtgcatgttc caatatatgg
    27001 agcagtggga ataatattgg cacaaattat atatgtattt cctcaatcat ttcaaatact
    27061 ttatatatct ctaagttcaa ccgattatag actatatgaa gctagtaata ctatggggat
    27121 aagcaagata agagaattta taagcataac aatcccaagt ataaaatact ctttaataag
    27181 ttctgcattc gtatcgttta cattatgttt tacagatttt ggagctccaa aggtgatagg
    27241 aggagagttt aatgtattat caatagatgt atataagcaa attatagggc aacagaatat
    27301 gccaatggga gcaactgtat caatattact tttaatacct gcaataatat cgtttatagt
    27361 aactaaaaaa attgaaaaaa atcaaaattc atatataact tcaaagtcaa ttccatataa
    27421 ggttaaaact aataagttaa gagatagtat tttaggaggg gttgtaggaa ttattgcatt
    27481 tggtataata gctgtaatgt taactgtaat cttatcttca ttaatatcag tttggccata
    27541 tgatttaaga ctgacactgg aacatttttc tatgaaaaac agtattgatg ggattaaacc
    27601 atttataaat tcaataaaaa tgtcactatt tacagctatt ctagggacga gctttgtttt
    27661 cttatttgca tatttgaatg aaaaaacaga aaaagctcca tatttaaaat catttgggta
    27721 ttttttaagt acattgccta tggcattgcc aggattagtt ttaggtatag cttatgtatt
    27781 attttttaat aaattagagt ttaattttat ggatacaata tacattaaga atttgtttaa
    27841 tggattatat ggaacattac ttataatggt tatatgtaat atagttcatt tcttttcagt
    27901 agcatttata acagctacta ctgctattaa gaaagtagat aaagaattag atatggtagc
    27961 taaatcaatg ggaataaaca gcgcatcaat tcttaagaac gtaactatac cactttgttt
    28021 gccagcagtt ttagagaact ttatgtactt tttcctaaac tccatggtga caatatcagc
    28081 attagttttt atatatactg caagttcaaa agtagctgca atatcaattg tacaactaga
    28141 tgataagggg agtactgcac aggcagcagc actagctgtg ttaatattag tcacaaatat
    28201 attagtgaaa gtaatatatg aatttataaa gaatcgttta gaaaaaagaa caaactttaa
    28261 ttaaataatt tatcttataa aaacacaaat ttaaaaaagt ctattcttgt gatttattat
    28321 ttaaagaata gactttttaa atttattatt gatattgttt attaattttc cttttgttat
    28381 catacatttg agaatctgca cgacgatatg tatcaaataa agaaacatct actttttcat
    28441 caaaaatagc atatccaata gacattgata attttaattc attagttgag ttttcttcaa
    28501 ctatttttaa attaatatta tttataatat tatttatttc tttgtgagat aaatttttta
    28561 gaataattat gtattcatca cctccaatac gataaactat atatttttta tttattacat
    28621 attgtataat tttacttgct tgcttaatat ataagtctcc tgtctgatgg ccaaatttat
    28681 cattaatctt ttttaaaccg tctaaatcta ttgatatgat agatattaga ttctttagat
    28741 ttgaattgtt taagttattc atatctattt caaaagcatt tctatttttt aggcctgtta
    28801 aataatctgt attagcaaaa tcagtatatg taggattaga tattctttta aatagaatca
    28861 ctgcaatcaa tgctgatatt agacaagtta ataatgctat tattggtgta actatactta
    28921 taaatttaaa tgtattgtat tgatgttttg cttcaaattc aattcctatt actccaacaa
    28981 ccttaccaac atcattgtga ataggaaaat acgatataaa tatgtatccc caagatgttg
    29041 atttaatctt tttaggtaac aatatatgat tatctaatgc tttgttaagg tctccccaaa
    29101 tctctttttc tatcaaatca ccagcatttc taaaatcact cctagatgaa tttaaaccat
    29161 caacaacata tactagtgat ccatcttttg tttttttagc agtatataga tacatgactc
    29221 cagtagtatt ttttacagat tctaaaagga ttttagaact tttataagac ttttttgatt
    29281 ggtcttcttt agaattaatt tcagtaaagg tatttttatc aatatttctt tctacatatt
    29341 catagatatg ttttgacctc tcacttaaag agtatatcat gctcttataa gttagtgtat
    29401 aattaaaaaa atatacacat gaggaagata ttattacaat aattgcagta agtattgaaa
    29461 cctgcatatc aactctattg aattttttat ttattttcat agcttaaatg tcttgtgaaa
    29521 agaacctccc acaatctccc cataattaat atatatctat aaatttcacc ataatatatt
    29581 catatattat caaatttgtg tggaaattac aattagtaaa catgcttaaa gcattcaaat
    29641 atagtttttt tattattata gtatactatt ttatgtataa ataaaattat atataaatca
    29701 ttgatattgg ttgtttatta atggaagata ccgtataaaa tattctaatt ggttacgata
    29761 tgtatactta attgaagtta agttaagggt tagaatatgt attgatgaaa tggctagatt
    29821 tatattcata agtattaatt ttatcataga taggtaaaat actgtatctt aaacataagt
    29881 atgattgaga tgcaatcctg agatatgata atataaattg aaggatagtg aactcaatca
    29941 aataaaatga ttaatctagc tatataagca aataaattta atataaaaga ggtggaaaat
    30001 gaaagacaga cttaatttaa cagaaggaag aattacagaa aaattactta agctatcact
    30061 tccaataatg gggacttcat ttatacagat ggggtataac atgatagata tgatatgggt
    30121 tggtaaagcc ggaagtaaag cagtagctgc agttggaaca gctggttttt ttccatggct
    30181 tgcaatggca tttattatga tttcaaaagt aggtggagag ataaaagtag cacaaagtat
    30241 tggtgcaaat aatataagca ctactaaatc atatatgaag tcagcaatag aaataaatat
    30301 tatactagca ataatatata caatttcttt aatagtactt aataaacaat tgataggttt
    30361 ttttagatta ggagacttag aagttataac catgtctaga cagtatttga taatagtagc
    30421 tctaggtatg gtattttatt ttattaatcc agtatttact gcaattttca atggtcttgg
    30481 aaatagtaaa actccttttc gtattaatac agtaggatta ataactaata ttatacttga
    30541 ccctatatta atttttggat ggggatttgc tccaaaactt ggtgttgcag gagctgctat
    30601 tgcaactgta tttgctcaga tagtagttac tacatgtttt atatatataa tagttaaaag
    30661 taaagaagaa tattttaaat taagactatt taaagaaata gagactaaat attataaagt
    30721 tctgtataaa ttgggattac ctatagcaat acaaagtggt atgtttacag tattctcaat
    30781 gctattagga gtaatagtag catcatgggg gcctgtggct gttgcagtac aaaaagtagg
    30841 ctcacaaata gaagcaattt cctggatgac ggctgaaggg ctatcagttg cattaggcag
    30901 ttttgttgga caaaattatg gtgccaagaa gtattctaga ataaataaag gttgcaaaat
    30961 agctataact gtatcttctg tacttggtat aataactact ttagtattag tgtttgcagg
    31021 agagagtata ttttctattt ttatagatga aagtgaggca atagaaaaag gagcagttta
    31081 tcttaaaata ttaggctatt ctcaattttt tatgtgttta gaaataataa caacaggagc
    31141 tttcaaagga ttagggagaa cctatatacc ttctattata attactatac tgactggtgc
    31201 aagagtacca ttggcttact ttatatctag accagaaatg ttgggtctaa atggtgtatg
    31261 gtggagtata accttatcaa gtatattaaa aggtatatta atgataagtc tatttatatc
    31321 tttattaaaa ctcggaaagt tgtacaagat ttctgaataa aatttaaatt atcttaaatg
    31381 tatagacatt ttctttgttt gtatataaaa ttatcttatt atataaaaga tttaataagg
    31441 agtgaattaa atgaaagagc caatttataa agttatagaa aatcatgtta gagagttaat
    31501 aaattcagac agtttgaaag aaggggattt aatcccttct gaaaaacagc tgagtgaaga
    31561 atttaatgta actagaatga ctgtaaggtc tgcacttaat aatctagtta aagaaggcta
    31621 tataactaga caaagaggtg taggtagtat agttattgca aataatatat atgataatat
    31681 atcgtctgta agtggattta ctaaagagat ggaaagtaaa ggctataaag tatctaatat
    31741 attggtatct cttgaaatag ttcaggcaga tgaaaattta tcaagtaagt tgaatataag
    31801 tttagaagaa aatgtttggg aaataaaaag agtaagatta gccaatgatg caagagtttc
    31861 atatatgatt acttatatgc cagttaagtt atttccaaat ttaaataaga ctcattgtga
    31921 aaattctctt tataattttg ttgaagaagt gtgtgaatac aagatagcca tgtcagagag
    31981 agaagtacaa gctgtaatat cgaatgaaga gtgtatggat aatctaaaat tagaagaacc
    32041 agagccactt ttatatataa gccaaatttg taagttacaa aatagtgaga tttttgagta
    32101 ctcacacaca tatcactatg gatatacatt aactttgaat gctgttgtcg aataaaattt
    32161 aaataaaatg aaaaagtttt cttgaaatgt atatacattt ggtatataat aatatcaaga
    32221 attaaatgta tatacattta aaaataagat atatacatta agaaaaaatt aatagaaaaa
    32281 catattttaa atgtatagtt taaaaaactt atgacttaaa aaatatttaa tgaatctaat
    32341 ttggagggga aaaaatgata aaaataatgt tagcttgttc agcaggaatg tcaactagtt
    32401 tattagttac aaaaatggag gatgcagcaa aggaaaacgg tatagaagca aaaatatggg
    32461 ctatatcaga agtgaactta aaaaatgaaa tagaaaattg tgatgtctta cttttagggc
    32521 ctcaagttag atatgtatta ggaaaagcaa ctgaaatggc aaaaccatat aatattccag
    32581 tagaagttat aaatatgatg gattatggaa gatgtaatgg aaaagctgtg ttagatagag
    32641 ctgtagagtt aaatagttca aaataaagtt gtactggagg atgtaaaatg gaaaaattca
    32701 tgtcatttat ggataaatat atagtgcctg tagctgctaa aataggagct caaaggcact
    32761 tagtagcagt aagagatgca tttatagtaa tgatacctat aactatggta ggtgcattgg
    32821 gaacattgat aaataactta ccattggaag catacaaaaa tttgatggca agtatatttg
    32881 gagaaaactg gaccacattt ggaggcgacc tttggtgggg agcaataggt acaatggcag
    32941 tattcttagt aataggtgtt gcttattttc tagcaaaatc ttatgagagt gatgggttac
    33001 aatctgggct tatagcttta tctatctttt ttataatggc tccacaaatt ggtaaaatag
    33061 ttcctgaggg aggaactaca gtagttgaag gatggggaat gatacaacaa acctacttag
    33121 gtacagcagc tttattctct tctatattaa taggattatt atcaactgag atatttgtaa
    33181 gattatctaa ggtaaagaaa ttaactataa aaatgccaga tggagttcca ccagcagttt
    33241 caagatcatt tgcaaagtta ataccaggaa tgttaactat aatgatattt actgtaatag
    33301 gaatatttat aaaaatgctt tctaatggaa gtttcttaac agatatttta aatacttatt
    33361 taggagcacc actatcaaat gtagctgata gtctaggttc tacaatgctt attgcattta
    33421 taattcatat actttggact gttggacttc atggtgcaaa tatagcactt ccttttacag
    33481 aaactatact tatgaaatta ggtggagaaa atgctgcctt ggctcaagca ggagcaacgg
    33541 aaggatacca tgtacttgca ggtgcattct ttgatgcatt tgtatattta ggtggttctg
    33601 gaatggtatt gggattaata gtagctttat taatagctgg aagaagacgt aaagagatga
    33661 tagtattagg tggtccacct gcaatattta acataggaga accattgata tttggacttc
    33721 caatagtttt aaatcctata tttatgattc catttgtact tgcaccagta atatgtagtg
    33781 ctgtatcata cttagcaata gattttggat tagtagcacc agtaatttta cctaaaatac
    33841 cttgggtaac acctcctata ttaggtggag caatggctac aggtgactgg actggcggag
    33901 ctttagcact atttaattta atattatcaa tattaatata tattccattt gtcatagcgt
    33961 ctgaaaaaat ggaagctaat aaattgaaaa ttaacaacta ggtagtttta tttcggtaaa
    34021 taaatgtata gagttttaaa taaatgtatg gggttttgta gagtatatta aattataggt
    34081 aaggatggta taatgagatg aaaaagggac taaaaatagt tacaattgga ggaggttcaa
    34141 gttatactcc tgagcttgta gagggtttta taaatagata tgaagagtta cctgtaaagg
    34201 aattatggct tgtagatata gaagctggaa aagaaaaatt agagatagtt ggaaatctag
    34261 ctaagaggat ggtaaaaaaa gctggagtag atatgaaaat aaatcttact ttagatagaa
    34321 gagaagctct aagagatgca gactttgtga caacacaact tagagtaggt cttttagatg
    34381 cgagaataaa agatgaaact attcctttat ctcatggagt tatggggcag gaaactaatg
    34441 gtgctggagg tttatttaaa gcccttcgca caataccagt tatatttgat ataataaaag
    34501 atgtggaaga attatgtcca aatgcatgga tggtgaattt tacaaatcca acaggaataa
    34561 taacagaggc tgtattcaaa tatactaatt ttagaagata tataggtcta tgcaacgtgc
    34621 caattcattt aaaaaatgat gttgccaaac tatttaatgt agaaagtgac agaataagta
    34681 tggattttgc aggtcttaat catatggttt atggtctaaa tgtatcttta gatggagaag
    34741 atgttacaaa agaagcaatt gataaatttg tcaaagcaga tataagtatg caaaatataa
    34801 aggcaattga ttttaatgct gaatttgtaa aatctttggg tgcaatacct tgtccatacc
    34861 atagatacta ctataaaact aaagaaatgc ttgaggatga gttgagagag tttaaagagg
    34921 gaaaagctag aggtcaagta gttaaagaat tggaagaaca attatttgaa ttatataagg
    34981 atgaaaaatt agatgtaaaa ccacctcaac ttgaaaaaag aggaggggct tactatagtg
    35041 atgctgcttg taatttaata agttctatat acaatgacaa aaaagatatc caagtagtta
    35101 atacattaaa taagggttct ataagagatt ttaaagatga acaagcagta gaagtaagta
    35161 gtgtaataac aaaaaatggt ccaaaaccaa taagtatagg atatttacca gattctgtgc
    35221 atggattagt aagtcaaatc aaatcatttg aagtattggc agctaaagca gctgtatatg
    35281 gcgattatga atctgctctt ttggcacttt gtataaaccc attgattcca tctgatgact
    35341 tagcaaaaac tatattagac gaaatgttgg aagctcataa agattatctt cctagattta
    35401 atagatagtt gtctatagaa agtaggttat ggtatgacta aaattataat aaatgcagat
    35461 gattttggat attgtgaagc tgtgaactat ggaataattt cagctcataa taatggtata
    35521 gttaggtcta cttctatgat ggcaaatatg cctggggtag aacatggagt aggcttactt
    35581 aaagaaaata aaactttaaa ttgtggagtt catatgactt taagttgtgg tagaccatta
    35641 ttatctaact taaaaactat tgttgataaa gacggttttt ttattaggag gattacagac
    35701 gaaattattg aaaaaatgga ttgtgatgag atttatagag aactttgtgc tcaaatagat
    35761 agggtaaaag ggcttggaat agatatatct catttagata gtcaccatca tatccatact
    35821 ttagtaagtt taaagccagt aatagagaag attgtaacca agtataatct tccaataaga
    35881 ggtggatttg aatataattt agagtattct aaagtagttc ctttaataga tagtttttat
    35941 aaggaaaatg taagtgaaga atattttata aaaaatatag acgaaataat gaagtatgat
    36001 gtagtggata ttatgtctca cccagctttt ttagatgatt atattttaaa ttctacatct
    36061 tatgctattg atagaacgaa agagcacaag atacttacat ctaaaaaagt caaagaattt
    36121 ttagaaaaga atggattagt catatcaagc tatagagata tttaggaggt cgaataaatg
    36181 gatgagaaga tgatagagat atcttttgga ataattggat ttgcaggtga tgcgaaaggg
    36241 ctagcatttg aagcaataaa agaagcaaag agcgggaata tagataaagc tagagaatgt
    36301 attgaaaagt cgaaagaatc aattcataaa gctcataaat tccaaacaga attgatacaa
    36361 aatgaagcta atggtaataa aactgaaata agtgttatac ttattcatgc acaagaccat
    36421 ttaatgacag ctatgaattt tcaacaatta gcagaagaat ttatagatgt atatgaaaga
    36481 ttagaaagta agtaatttaa aattattatt aaaaaagagt gtataaaaat aatttttata
    36541 attattaaat gcactctttt tttatttaag tagagtttag caatatagta ttatgttatt
    36601 gatactttag aatttagact tctcataatt gtaaggtata ctagtaccaa caactacatc
    36661 ttcaattttt tcaacaataa gccaatcatc catatttcct tgatggtcaa aatcaagagg
    36721 tgttatttct tcaacatttt caaattcaac taagcataaa cattttttat gccaacgaac
    36781 tttttgcttt tcagataaat ttaattttgg ttgattttca tttaaaattt tagaaatttc
    36841 atcttctgtt aatttaacat agttttctac ctttttaaca gtggcttttg cagatatttt
    36901 tgcagttcct ttttccataa aataaagtaa ctcatcttca aatactctac tatgtggtat
    36961 ttttctacca gcagcacctc taataaccat tgtttttgtt ccatccaaga ttttatctaa
    37021 gaccttttct cctttttttc ctgcattatc acaatatact aaatgtacca tgttttgaat
    37081 cctccctact aaattcctta tctataaatt gcattttatc attgtttaac tgaaaagtca
    37141 ttgtatttgt gtccaattct taaaaaaaat actgtttaat ataagaaaac cttatctaag
    37201 ctatatataa ttaagattaa ttaggaattt ttatcctttt acaaaataat attgactaaa
    37261 aatagaaaaa tgctataata aatatgtgct tatgataatc aatatcatgt aagtataata
    37321 aacaagtagg agaagtaatc atgaaaaaaa taaaatcact tgcaatattt atatctatta
    37381 taactacgtt agtacttgtt acagcatgtt cagataagaa tacagaggat aaagataaat
    37441 cagagacaag agttgtacaa tctgttaaag gagaggttaa gataccatca aatcctaaaa
    37501 aaatagttga tatatcagga agtagtgaag aactattatt agcaggttat aagcctgttg
    37561 caacagcaaa tgtagactca tatgaaactg ataaattacc aagttacata agagaagagc
    37621 ttaaaggtgt aaagatagtt gggcactcaa tgatggatac tatggatatg gaagctatat
    37681 tagaagtaaa tcctgattta ataataatga gtcaaagaca agaaaaaata tatgaccaat
    37741 taaaagaaat agctcctgta gttatgatga aagattatgc aaatgactgg agaagcaagt
    37801 taactgatgt ttcaaagtta tttgacaaag aagaagaaga taaaagttgg ctacagaagt
    37861 atgatgaaaa agctacaaag ttaggtaagg aagttataga aaagaatgga gaaaaaactt
    37921 atttaccagt gttggcaagt tcaggtcaat ttatggtatt tagtgatggt ggaataggta
    37981 ctctaatcaa tgatgatatg aaattagcta gacctaagaa tatgccaaaa caggatggaa
    38041 taacactacc aatggtaagt atggaaggtt taacagatat agatgcagac catatagttg
    38101 taattgctac agaagcagat aaaaaagatt tagaaaatag tgctatctgg gctcaaataa
    38161 gagcagtaaa agatggtaat gtaacaatac ttgatgctgc tccatttttc agtcaatcat
    38221 acaatccaat tggaaaagag ttattattag aatcagttaa aaatgaatta acaaagtaat
    38281 tcttaactta gtaggtgtga gataatgata ttcatttttc acacctattt ttaataaatc
    38341 ttaaaattaa aatacttaat agatgatatt atactgacga ttaaaaggga aaaggaggta
    38401 ggataattga ctaagctgag taaaaaaaat ataacttatt tgttgatatt agtaagtata
    38461 gttgtacttg tatttggaat tgtattatcc ataactattg gggcaaaaga tatgaatcta
    38521 tcaactgtta tagatagttt gataaaaatg gaagatggaa taaatatgag aatagtaaag
    38581 gatgttagac ttccaagggc aatagcagct gcacttgttg gagggttttt agcagtttct
    38641 ggggcaataa tgcaaggaat aacgagaaat cctatagctg aaccatctgt tataggaatt
    38701 acacaaggag ctacattcgc aatttcgata tcactggtac ttcaaaaaaa acttcctcaa
    38761 cttatacatg gaagttttag tgtaatgatg tttgcattta ttggagcaag tataagtgga
    38821 ttatttatat attttataag ttctaaatca agaggaagag ttaataatgt aaaattagct
    38881 cttgcaggag tcgctcttgg tacattgcta atttctttgg catcagcaat atctatgtat
    38941 tttaacctat ctcaacaatt aagtttctgg atatcaggag ggcttgtagg agtgaagtgg
    39001 gaaggtataa aattgctatt tgtagctggt ggtattggat ttattttagc tattattatg
    39061 gctccaagaa taactatatt aagtcttgga gaagaagtgg caataggatt aggacaaaaa
    39121 actaagtttg ttagatttat atgtatagta cttgtaatat taatgactgg agcatctgtg
    39181 tcagtgtctg gtaatattat ttttataggt cttatagttc ctcaaatagc taaaggaata
    39241 gtaggttcag attataagta tataataccg agctctttag tattaggagc tgtattgctt
    39301 gtatatacag atatattaag taggatgata aatcctccat atgagacacc agtaggttct
    39361 ataacagcac ttataggagt gccaatattt atataccttg ttagaaaggg ggagaaatag
    39421 gtactatgaa aaatattata aaaataaatt ctaaaacatt acttgttata tcaattttaa
    39481 tggtgcttat ttttatcact tttttattga gtttaaatat gggctcattt tctatagaac
    39541 caatggatgt tataaagact tttttagggc agggtcagcg taatcatgaa attgcaatat
    39601 ttaaattaag attaccaaga atagtaatag cacttttggt tggttctgca ttatcaacag
    39661 caggtgtaat acttcaagga gttacaaaga atgatttagc tgattctgga attttgggaa
    39721 taaattcagg tgctgcattg tttgtagtag tttatatata ccttatgaat ggtaatgtat
    39781 atgaaggtat gagtaacttg actgtattta caatgcctat tgttgcactt ataggagctt
    39841 tatttggagc atttcttata tattctcttg catggaaagg tggtataaat tcgtcaagat
    39901 tactacttat tggtataggt gtaaacatag cttttacttc aattttgacg atattccaat
    39961 tgaaatttac aacacaagaa tttaataggg ttatggcatg gacatcagga agcatttggg
    40021 gaacaagttg gaaatatgta atagctgtgc ttccatttat agtgattttt atgggtataa
    40081 ctatttataa atctaaatat atagatgttt taaatttggg tgatgaagta tctataagtc
    40141 ttggtgtaga tgtagaaaaa caaagaagaa gacttataat atatgcagta attttatctg
    40201 gtgtatcgac agcagtagca ggtagtattt catttttagg tttaatagct ccacatatag
    40261 gtagaaaaat aataggacca aagcataaaa gattaatacc agtatcagca ttgataggaa
    40321 cattattact tttaatagca gatactatat caagaaatct tctagcacct atagaaatac
    40381 cagtaggtat agtagtttct ataataggag tgccgtactt tatttattta atgttggtaa
    40441 atgattagga aagggtgttg ttatgaactg tataaatact aagaatctaa atgtatcata
    40501 tggaaacaca gatattataa aaaatttaaa tttaaatata ccaaaaggaa aaataactac
    40561 catcataggg gctaatggtt gtggaaaatc gacaatactt aaaactatag gtagaattat
    40621 tagccctaat agtgggaaaa tatatataaa tggagaggac atctacaaaa agaagtctaa
    40681 agaaatagca aaagatatgg ctgtacttcc acaaagccct caagctccta gtggtcttac
    40741 cgtttcagaa ttaatttctt atggaagatt tcctcataaa agtggatttg gtaattctag
    40801 taaagaagac agagagattg taaattggag tttagaagtt actggaattg cagaatttaa
    40861 ggatagaaat atggaagatt tatcaggagg acaaagacaa agggcatgga tagctatggc
    40921 tttggcacaa gaaactgata tattattact ggatgaacct acaacatatt tagatttggc
    40981 acatcaattg gagatactaa aattattaga tgaactaaat aaaaaacaag gaagaactat
    41041 tgtcatggta atacatgaac ttaataatgc agcaagattt gctgaccaca tgataggtat
    41101 taagaaaggt aaaatagttt gtgaaggtga tgcatatgat gttatgacga aggataatct
    41161 aaaagaatta tttaatattg atgcagaaat agcaacagac ccaagaacaa ataaaccaat
    41221 ttgtataact tatgacatgg tgtaaatggt gacaacatgg agaatcaaga agcattaaga
    41281 catgaaaaaa tatggatttt attatttagg tattcgatac ctgcaataat agcaatgatg
    41341 gtaacttcac tttataatgt agtagataga gcttttattg gttctatgga aggtattggc
    41401 tctatagcta ttgcaggtct tggtgtaact atgccagtat ttactctaat aatagctttt
    41461 ggaatgttgg tatcagtagg tgctagcaca agattgtcta taaagttagg ggaaagaaat
    41521 agagaagaag cagagaagat attaggtaat gctttgacat tatctataat tatctcttta
    41581 attataacta tattaggatt agttttctta gaagagatac tttttatttt gggtgcaagt
    41641 aaagattcaa tattttatgc aaaagactat atgagtgtca tattggttgg aagtatattt
    41701 aatttagttg cattttctct taataatgca attagggcag aaggtaatcc aaaattagca
    41761 gcaaggacaa tgattgtagg atgtgtatta aacctgatat tggaccctat atttatattt
    41821 gtatttaatt taggtataaa aggagcagct atagcaacag tactctgtca aatagtagta
    41881 tttttttggg taatacatta ttttataaga gggaagtcca atttaaaact aaaaaaatgt
    41941 aacttaaaat tgaataaaga tatagtaaag aaggtatttg caataggaat gacacctttt
    42001 gctatggaag tagctataag tataactcac atgtttacaa ataattctct aaaggtttat
    42061 ggtggagatt tagctattgg agctatgaca gcacttacat ctattttatt gatgtttatg
    42121 atgcctgtgt ttggattgaa tcaaggtatg caaactataa tatcatacaa ttatggtgca
    42181 aaacaatatg agcgtgcaaa gaaaacttta atattatcta tcatagtgtc agttgtcata
    42241 ttgtcatttg gatttttagt agtacaagtt ttcccagaag tatttgtagg tatatttaat
    42301 aaagattcag atttgatgga tatagcagta agaggtataa gcctaaatct aattacattg
    42361 cctataatgg ggatttctat tgtaggacct gtgtattttc agtgtataag taaagttaaa
    42421 cattcaatgt ttttaactct tttaagacaa tttattttat ttataccatt aatcattgta
    42481 cttccaattc aatttaattt agatggtgtt tggttggcac agccagttgc agattttatt
    42541 gctatgataa tcgtattatt attcttaaaa agggaattta aaacaagccg agtgtaagaa
    42601 atttagaaaa taaaattaaa ttggaaaata aaaagacttg tctttataaa atgattaaaa
    42661 ttattttata gagacaagtt ttttatttta tttaaacgga actatccata ctggtttctt
    42721 ctagtttttt tccaaaagtc attttctcaa atatttcttg aggtagaggt tttgaaaaaa
    42781 catatccttg tatcatatca caattaattt ttttaagata atccatttgc aagattgttt
    42841 caacaccttc tgatatacta ttcatattta atttttttgc caaattaact atagattcta
    42901 ttacatgatt ttcagcaata ttatctgcat taggtgagct aaaaaaagca cggtcaagtt
    42961 ttaatgtgtc aacttttatt tcttttaaca tattgagtga agagtatcca cttccaaagt
    43021 catctagtga acattgaaaa ccttcttcat ggatttggtc tattatatta attaattttt
    43081 ggagattttc aaatacaagt gtttcagtta attcaatttc taagagctta gcaggaatgt
    43141 tgtatttatt acgaagcatt ctgtattttt ctaaaaaatc tatattattt aaattagcac
    43201 gtgaaaaatt aactgaaaca ggtattggag tttctccatt atctatccat tttcttatca
    43261 tactacatac tttttcaaaa acatagtggt cgagttttgt aataaaacca tttttttcaa
    43321 agaaaggaat aaactcgtct ggtggtataa ggcctttatt tgggtcttgc catcttacta
    43381 atgcttctgc acctacaata gtattatctt tgaggcttat tttaggctga agatatacta
    43441 taaattcgtt attatttaat gcttcctcca ttctattttc catttctttt tctttaatca
    43501 tttttaaacg ctctaaatca ctataaaaaa cacatgtata aagtggatga tttccaatgt
    43561 ttcctttatt attttttcgt gcaacatttg cacggtcttg aattgaaact aaaggtagat
    43621 ttgggtcatc aattttatat acacctatag atagagctat aaaatattta cattcttcat
    43681 ttttattgaa actattaata tcattggtga tattttctat gtacttaaga atttcttttt
    43741 gaggaatagt tttaataagt acattaaact ggtcattaga aactcgacaa agcaattctc
    43801 ctggttctag ataattacta atgacatcat gtatgtactt taatgtttta tctcctgctt
    43861 cactaccaaa agaatcatta ataagtttga atttctgaat atttatagat accaatgaat
    43921 agctttctga tgaagatgac tgtataattt ttttagcttc aatatcaaaa cgtgctttac
    43981 tgtaaccttt agttacagag tctacatagg cgatttttat aagttgtgcc ctgttttttt
    44041 gatttataaa cataatcaat ataataagca ataaaaatag tataacaatt aaaatattga
    44101 tgaagattgc tttttttaga agtaaatcaa actgtgcact tgctgttttt gttggaacaa
    44161 ttgaaaggag gtataagttt tcttcttgta atggaagata gtgcattgcc ttagaaatac
    44221 catcatttaa tgtataatac atagaaccag ttttgttctt ttttaagttt attttcattt
    44281 cttcaagttt atttttatca acctgttttc cttggtgttt aatcatgtca aaaaaattta
    44341 ccacattttt tggagcattt ttgtttgctg aacttacaat aaaatttcca tcttggtcga
    44401 taatttggga aaatccttca ccatgaaagc tttctacact taataaattt ctcatagtat
    44461 caatagtatt ccaagcagca acagctccaa caattttatc gccattgtat agtggtacag
    44521 aatataaaat tccatatgta ccagattgtg atttaacagg agtaattgtt tctttgcctt
    44581 gaagggcttt tctataaaag tcttgtcctt taacattacc tgtatcttta agggcactta
    44641 caaaatttcc attagaatct ataataccta accaatcaaa ttcaaataat tttaattttt
    44701 ttgataagta tctattgact tctttttcac tatcattaat ctcaaaataa gtatctccca
    44761 tagaatatag tgtcctaaat atacttttac atttcacatt aattgtagtg gaaatacctt
    44821 gtgaaacttc tacaagatat cgttctgttt cattatgtgt tactttattt agattcataa
    44881 taaaagtgac acatgttaat gacacgatta aaagaagtgt caatagtaaa gtagctatta
    44941 tttttttatt ttttgtgttt tcaaagttca ttcttttgtt tcatcctcca tcctttattt
    45001 gtttacaaat aagaattctc tcagacatat tataccaaat atcttttcat acaaagatat
    45061 taaattttat ttatcccact ttacatttag ctttatttat gtcttcttag agattaatca
    45121 agatacgttt attgtttaaa tcttatttta taatattttg agtaaaagag gttaaaaaaa
    45181 gaactgtttt caaaataatt ttgatacagt tccttttcag tatagaaaat ttttatagta
    45241 ttagaaagaa cttttagctt atgtatctac ataagtatta tatttataaa agatattttg
    45301 ttacaatcta aaatgtagat gaaattctgt aaatgccaaa ttctctatga ccaagacttt
    45361 cagctttggt atgtttacca ttacagactt ctattatttc attaaatatt tcttgaccta
    45421 tatctgctat tttagcacca tctgttataa ttctacctgc atttatatcg atattgtctg
    45481 gcattttgtt gtatgtatca ctattcccag tgattttaat aactggtgaa atagggcttc
    45541 ctgttggagt acctctacca gttgaaaaga ttacaatttg tgcaccacct gctaccatac
    45601 cagtgataga atctatatct tgacctggag tatccataaa atataatcct tttttatcag
    45661 gaggtacaat atcagcatat tcaagagcac ctttgaatgg cttattgcct gccttataca
    45721 tacacccaag tgacttttct tcaattgttg aaagtccacc agttttattg cctggagtag
    45781 gttgaccact acgtaaatct tctcccatag caattgctct tttttctaca tcacttacaa
    45841 atttaagaaa cttatctttc atttcttcat cttcaaaacg ggtagccaac aaatgttctg
    45901 caccaattac ttctgtagtt tcacttagta tgctacttcc accttcatct acaagaaggt
    45961 ttgatgcaac gcctattgaa gggtttgaag ctattccact tgttgggtca gaaccaccac
    46021 attcaagtgc taatgtaatt tcactcatac caaattcaac tcttacttct tttgatactt
    46081 cacgagccat tttacttact atttcagctc ccttagaaac tgtttttaat gttccgccac
    46141 attcctgtat aacaacgtaa tctacctttt ttcctgttgt agctatttca gatgctaatt
    46201 cttttgcttg aataccatca catccaagac caactactaa aactgcggca acattaggat
    46261 tttttccaaa tcctattaat gtctttgctg taagctctat atcagaacca atttgacaac
    46321 atccatgttg atgagggatt gctattgctc caggcacaag tgatgctatt cttgtagctg
    46381 tctcacttga acatacagat gttggaataa ttaaaacatg attacgaata ccaaactgac
    46441 cattttctct taaataaccc attaatttca ttttaacttc ctcctatttt ttatctccac
    46501 gaccacgtgt actttcaatg ttatgtacat gaacatagtc accagttttt atgtcaactg
    46561 ttgcaattcc tataatttca ccgtatttgt atacaacatc accttttttt acatcttctg
    46621 tagccatctt atgaaagact ggtatattat catttactat gtaagatttt ccatcaattg
    46681 aaactttttc acctttaact aaaggtctga cacatgtaac tagattatct ttttcatttg
    46741 catgaataat tgcattcatt aattttacct ccttaagtat taatttactt caatattttt
    46801 atttgtttta aaaatagatt ttttcttttt ttctctttta tctagatagg aaactaacat
    46861 tggacaacat attgctgtaa caataatagc agcagcaacc tgtactgttg aaacagctgc
    46921 tattgtagct agagtagggt ctatagctgc taaagcttca ggagtaccag ctgcatttcc
    46981 ggctgttgta cctgtagctg cacctactgc tggatgttct gcacgtataa gtttcattac
    47041 aaagtaccca cccatacctg ttattatagt aactcctaaa ccaagtacaa ctccaggtag
    47101 tccagcagtt ataatttgat taaagtttaa tgctgctcct aaaggaaaag cgaagaaagg
    47161 aattaataat gttgtaccag gtgcaagaaa ttctcgtata tcttcatcaa ggttaccaag
    47221 tataaatcca agtagtattg gtataagtgc tgcaaatagt gctattatag gtacatttgc
    47281 aagtccagtt actccaaaag caagcattgt aaaaaaagga ccatcattta gagataagat
    47341 agatattgct ccaacatctg tggagtctcc atattcacct gcaagagcag caaatagacc
    47401 tccatttgaa ttagtcatgg tagcaactat ggcaagaggt gtaataccaa ttataccttt
    47461 aggtccaata aatttactaa caataatacc taatactgca ccaattataa attttgttgc
    47521 tgtaagtact atacctttag tcaatggttt accagcttgt ctgatattga tttgagctcc
    47581 attgcataga caaaatagag caagtatact cattgcacca gttttaaata aggatgttgt
    47641 gaatcctcca atttcaagag cttgtggaca tactgtatta aatagtgctc ccaataacaa
    47701 gggacagacc ataagtcctc ctggtatctt tttcactgat tttaagatgt gcatctagta
    47761 tcactccctt tcatttttat ataggtatta taaaagcaat taatatgcca atatatagag
    47821 tttgtatctt tagttatcat gattattttt aataatcgat tgaaatgctg tagattactt
    47881 ataattaaaa gtacgattga cgagaatttc acttacaaag attcttttta tcatataatt
    47941 gtatttttta ttgaaaacaa ataaacgtct tatgtattaa aattaaacat aaaacgtttt
    48001 gatatatata ggtatgtatt atttttaaac atcatcttct atttctttta acttacgcca
    48061 tagagtggag cgagttatct ttagaatttc acaggttttt gtagtatttc tattattttc
    48121 gttataaatt ttatagatat attttttttc taaagttttt aaatctaaat tactgcattc
    48181 aaaatcacta ttatctttat caactatatt atttgtatat tcattgctaa ccttttctaa
    48241 taaaatcgag tcaaatgatt ccacaataac agccctttcc atcatacctt ccaattccct
    48301 tatattacct tcatattctc gttcaagaag tgccttttta atatttggag tgataattaa
    48361 aggcgtttta ttgtatttgc ttgaatattt atgtataaaa aattctgata attttataat
    48421 atcttctttt ctttctttta atgaaggaag atataatgtt agtatattta ttctaaaata
    48481 aaggtcttgt ctaaataaac cattgcgcat catatttttt aagtctttat ttgaagcaca
    48541 gataattttt acatctatag gaataacttt atcaccacca attctcataa cttgtttttc
    48601 ctgcaatacc ctcaataatc gaccttgaag ttcaagaggg atttcactta tttcatctag
    48661 gaagattgta ccttcatgtg ctagttcgaa tacacctgct ttaccttttt ttctagcacc
    48721 tgtaaatgaa ccttcctcat aaccaaaaaa ctcactctct attagagatg gaggtatggc
    48781 agcacaattt acagcaacaa aaggtgcatt tttacgctta ctatagttat gtatactttg
    48841 acaaaatagt tctttaccaa ctccagattc tccagtaata atcataggag tatcataaca
    48901 agcatatttt tttgcaagtt ctatacaatc ttttgtcttt tcactagcat gaattatatc
    48961 atcaaaagtg tatttagcag atagaccttt gcttaataag ctccttctta ttgtatgttc
    49021 aaaagaactt atctccttgc tatcttgaaa tgttgaaaca gccccttgaa tctgaccatc
    49081 aacaatgatt ggaactctat ttgtaataat ttttgtactc cctatatttt gtatttcacc
    49141 aatttgaggt tccatagtct caactacttg tggaagaact gtgtttggga taacatctac
    49201 aacattatga taaagtgcat tttctttttt tataccaaat attttttcag cactcttatt
    49261 aaataatgtt attttaaaat ttttatcaac tgttattatt ccatcatgta caaaatctgt
    49321 catagcttct atctgtaatc ttcttttttg ttcatccttg gttgcttcga gtatttgttc
    49381 tgcttgttga atggctctaa gtatgttttc ttctctagat tcgattaata ttcccttaca
    49441 attaagtctc ttaacaatat gtgcaacagt ggtatctcct atatatgtgt ttataccttt
    49501 ctttctgtac ttttctatag catcataaat ttcttcttct ttttcaattt caatcatagt
    49561 tattttctta ttcaatattt cttctataat atcgaaaccg tagatgacat tattaaatcc
    49621 aattattcca aatggttcgt tggaattttt tacctctttg taacttttta taatatcata
    49681 agcatctacc ttaatttcaa ctataggtat attgtaagta gcttttaaca tattgtaagt
    49741 tccacctcgg cttatgataa ttctagtacc atgattaata cttttttcag caagaggtat
    49801 accttctttt agattgccaa atacaacatc aatatttgta tatttgtttt ttttaactat
    49861 gtgaattgat ttttcatagg ttcctttagt gggagcaata acgagtattt ccttcataat
    49921 aattcctcca tatttcatca aataaactta aatattatct tatgactaaa ttttatcagt
    49981 tatattttaa cattttactt tatatttaat tataaataaa gtatttttaa aaggaacatt
    50041 ataaaagact gtatcaaagt taacttttag aattataaag tttcatcttt ttacagtctt
    50101 ttaatatata atatctattt atcaaatcta tctactttaa ataatttcat atttttattt
    50161 tgtttgccaa gatataattc acttaacatt atacctccta atattgcaaa gagtatacca
    50221 tttgctccat aacctaagca ataccataga ttgttatttt tattatctgg accaataaaa
    50281 cctaaattat ctaaagttga agcaaaacaa ccacaatatt tatattcaac ttcaatgtct
    50341 tttatatcct taaacatact tttcaatctt tgttctaaaa tatcatactt ttcattggct
    50401 aaagtttcat tttctatatc atcaaaactt acatcttcac cacctataat taatcgattg
    50461 tctttagtag ttcttagata attatatggg tcacagttat ctctaattaa aatgttgttt
    50521 ttccaagaag ctatgttttt aagtggtttc gttgctatat taaaagtatt tgacttagtt
    50581 gcaaaatttc tttttgtaaa taaacttgta ttgtaccctg tagctacaat taatttttta
    50641 ccatgaactt tgtatccata cgatacttca gcagtaacct tatcatttga taaatctact
    50701 ttttttactt ctgtattttc atatacttga agaccatttt ttaaacttac atcaatcaaa
    50761 tggtgactat atttatatgg gtctaattct cgtcctccat tctttgctat caatcctgat
    50821 tttaaatcaa agttaaaagg gtttgtggat tcatcaatat attcaacatc aaatccattt
    50881 tcttttctta agttatattc ttctttgata gccttaactt caagtttgtt ggcagtataa
    50941 agtagagtat ctcttttggc ataatcacac ttatttccat atagctctat aaatgtatct
    51001 aattcttcaa gtgctgaagt acaaaggtta tatgctttta gagcatcttt tagagtcata
    51061 acctctgtta aatctatcaa attgtcatct aattcatact gtaatagaga agttgtcacg
    51121 ctagtgcttc catgtgcaat ccttcctttt tcaagaatga cagactttat attgttttta
    51181 gctagatagt aggcacaaat acatccagtt acgccaccac caacaattat aacatcagta
    51241 tcaatatcat cagtaaggta tggaaattgt cttggaactt tattaatatt ggtaaaaata
    51301 ggatttcctt ttacatattc ttgattcata tacttcactc ctaaaattta ttatgtatga
    51361 gtgtattatt ttctatagca aataatatta tacattttaa ttatctagtt atatgggtat
    51421 gtatagataa aatgtgataa cattttagta taaaacatat gtataatata ttcataatta
    51481 ccttatttta agtaatatag tatataatgt ttgttaacaa gtaaaaagat tgaataaaag
    51541 ttaattaaaa atttaaagta ggaggtaaat ttatgctttc aaaaaatata gcttctaagg
    51601 tattagaaaa atgtctaatt actggaggag attttgctga gatttttgaa gaagacacta
    51661 taaataattc tattagttta atagataata aagtagaaaa tgcaataggt ggaagaaatt
    51721 atggtatagg aataagaata tttaaaggat tgaaaagtat atatgcttat acaagtgaca
    51781 attcattaaa tagtctgttg gatgtagcat acaaagctgc tgttgcttta ggtaaattgg
    51841 aagatggaaa aagtgtaata ttaaatgaca gtattaaaat aaataacata cataatataa
    51901 aaatttatcc aaattcaata ggaaataaag ataaagtaag tgttatgaag gttgcatata
    51961 agagtgcaaa agaatttagt aatgatatat ctcaagtaag tgttagctat ttggataaag
    52021 accaaaaaat attaattgca aacactgaag gtatatatgt tgaggataga agaataagaa
    52081 ccagactagg gataagttca attgcatcta agggtaatga aaatcaaact ggatttgaag
    52141 gtcctggggg ttgtaaaggg tttgaaatat ttgaagaaat agacccagaa tattatgcaa
    52201 aagaatcagc tagagtagcg catactatgt tgcatgctaa aaattgtcct gcaggtaata
    52261 tgatggtagc aatagataat ggatttggtg gagtaatatt tcatgaagct tgtggacatt
    52321 ctttggaagc tacatcagtt gcaaagggga actctgtatt tgcagataaa ttaggtcaac
    52381 agattgcttc cacaaagatt acagctatag atgatggtac aataccaaac tactggggtt
    52441 ctatgaatat agatgatgaa ggaaatccaa ctcaaaagaa tatactgatt gaaaatggaa
    52501 ttttgaagtc ttatatgata gataaactta atggtagacg aatgggaatg aatcctacag
    52561 gaagttctcg tagacagaac tacaaatttg caccaacttc aaggatgact aacacttata
    52621 ttgctgctgg agaagataga ccagaagata ttataaagtc aataccagat ggactttatg
    52681 ctaaaaaaat gggtggtggt tctgtaaatc ctgtaacagg agagtttaat tttgcagttt
    52741 cagaaggtta cttaataaaa aatggagaaa tacaagagcc agttagaggt gctagtttaa
    52801 ttggaaaagg tagcgatata cttatgaata tagatatggt tgggaataac gtaaaacaag
    52861 ctcaggggat gtgtggctca tcatcaggaa gtataccaac taatgtaggc caacctatga
    52921 taagggtaaa agaaattact gtaggtggaa gataggaggg ataattgtgg aacttaaaaa
    52981 ttttaaagat atattattta aaaaggctct aagtgaaggt tttgaagagt atgaaatata
    53041 ttattataca ggagaaaatt taagtataaa tatatatgaa ggagaagttg agaaatacaa
    53101 tcttgataaa tcttttggtt tatcttttag aggtaaggta aatggaaaga taggttattc
    53161 ttatacagaa attttagatg ataaagctgt tgatatgttg ataaaaaatg taaaagatgg
    53221 agtaaacact attgaaaatg aagatgtaca atttatatat gaaggagata agcactataa
    53281 tgatgtaaag acttattcta aagagctaga aaatttagag gcagataagt tgattgattt
    53341 agctttagaa atggaaagag agactaaagc ttattcagat aaggtggtaa atttatctaa
    53401 atgtactata tcttattctg tatcaagtaa tggtatatct aatactaaag gtcttaattt
    53461 aagcaataaa actaatatgc ttatagggtt tgtagttcct atcatagaag acaatggaca
    53521 aaaatatgat ggaataggat ataatatagc aaattctatt gaagaaataa aaccttgtga
    53581 gatagcaaaa cttggtgttg ataatgcttt atctaaagta ggtggtaaga gtattccatc
    53641 aggtaaatat aaaactatat tgcttaatga agctatggta tctttgctta gcacattttc
    53701 tgggatattt aatgcagatt cagctcaaaa aggattatct ttattgaaaa atagagaagg
    53761 agacatgata gcatctccta tagttacaat agttgatgac ccacttttag aaaatggtat
    53821 ggcatctact ccttttgatg atgaaggagt agctacattt aaaaaagaag ttgttttaaa
    53881 tggaaagctt ataactttac ttcataactt aaaaacggct aataaggcag gtgtaaagac
    53941 tacaggaaat ggaataaaat cttcatattc atcacctata agcatatatc caactaattt
    54001 ttatatagag aaaggagata aatctttaga tgaaattata aaggatatag atgaaggatt
    54061 gatggttact agctttgcag gacttcattc aggtgcaaat tcagtaacag gagacttttc
    54121 tttagctgcc aaaggctttt atatcaaaga aggaaaaaaa gtatttccag tagagcaaat
    54181 tacagtagca ggaaactatt ttgacctgtt gaaagatatt gaagctatag gggaagattt
    54241 agaatttcca atgagtagta taggttcacc ttcagttgtt attaaagagc tatcagtagc
    54301 tggaaaagat gaataattaa aaagtaattt tgtggataaa aggtcatgtt aattttaatt
    54361 aatatggctt tttattattt aagtagattt tattatctaa gttatatgaa aaagtcgtga
    54421 tgcatacaat taatatcatg tattaaattt aaaggaggag atattatgag tgatgcacat
    54481 tatgacatgc acaagcgaaa tggatattca agtgatgagc catatccaga gataaaagtt
    54541 ttaggtccaa ataagtatta tgcagaatta ttgatggatg actatgccgg agtatcaagt
    54601 gaatttacga gtgtaaatca atatctatac cataattttg atttagatga aacgcataga
    54661 gagttaagtg aaatgtggat aaatatatct ataacagaaa tgttacatat ggagatatta
    54721 gcaaagacta taagattatt gggtggaaat cctgtatata gaggttctac aagttcttgt
    54781 ggtgcatatt ggaatggtgg ttttgtatgt tatggaaatt ctatttgtaa cagattaaaa
    54841 ctagatttgc atttggagca tgtggctatt aataattatt ataaagatat aagtcttatt
    54901 gaagaccctt atataaaagc tatattaaat agaataatcc tagatgaaaa acttcatgta
    54961 agtttatttg aaaaggcaat agagaagtat tgtaaataaa tttgaaaatt tagttattca
    55021 tcattttatt catatatata aaataaccat catacatttt cttgaaggga gttgctgttg
    55081 atatgtatta tgtatatgtg aatgatataa acatagcagt atatgatttg aatccgagtg
    55141 ctaaaaaaac tgtacttttt atacatggtt ggcccttagg acataagata tttgaatatc
    55201 aaacaaatat attgccaaaa ctcggatata gaacagtttc aattgattta agaggttttg
    55261 gtaaatctga tgcaacatca ggaggatata cttatagcca attagcagac gatatttata
    55321 aggtggtaca tgctattgga ttaaaagact ttacccttgt tggtttttca atgggaggag
    55381 caatcgtact taggtatatg agtttattta atggatatgg agtgtcaaaa ttggtattag
    55441 ctgcagctgc tgcaccatca tttgtacaaa gacctccaga atttccatat ggtatgacac
    55501 gtgaagatgt aaataaattg atagcacaag catgtactga tagaccagaa atggtaaccg
    55561 attttggcga aaaagtattt gcatcaaatc caccagaaag ctttagaaga tggtttaatg
    55621 atattggttt tagtgcatca ggtattggca ctattggaac tgctgtatct ttaagagatg
    55681 aagaattatt taatgattta aaatgtgtaa gagtgcctac tggtatattt catgggaaat
    55741 tagatgaaat atgtccgtat gaatttgcag tttttatgaa tgaaaaaata gaagattcaa
    55801 ttttatatac ttttgagtat agtggtcatg ctatattcta tgatgagctt aaactattta
    55861 atcagaaatt tttacagttt ttagaggaat agataaattt tattatcact tagaaaaaga
    55921 gtaatattat gtgaaaagat ttaagctaaa ttttatgatg ttttttacac taaacaaata
    55981 gggttttaga acttgtaact aagttctgag accctattat tatatattcc taaaatttgt
    56041 tataatttat tccttgtcat tcttaaaaat gtattaatat attaacgaat ttatattaaa
    56101 tttttgttta tattccaata atgtcaaact aaccgtttta gaaattaaat tatatgtgaa
    56161 aaaagatagc ttacattatt ttattatgga aaataatagt aaatggtaca aaatttgatt
    56221 tttaagattt aaaacttttt taaaaagtgc tacaatagac caaattatta ctttctatac
    56281 tcgttaaaca ggaggtggga ctatgaaaaa acataatatt ttatttgtat caactgatga
    56341 caaaataaat atagatatat caaaacaatt ggaaaatata tttggagaat tttgtaatat
    56401 agataacttg gtatatgtaa ataggataaa tattgaactg tcaagctatg agcttgtagt
    56461 atgttcagac aatgatataa aggaatatat acataataat attgataaaa atatacctat
    56521 tgtaatagta catcgtacta taaatattga aaatataaat cagataataa gtatagaaaa
    56581 tgattctgat gttatggtta tagatgctta taaagaatca gcagatgaaa ctgccaagat
    56641 aattagaaaa ctaggattaa ttcatattaa tctaatacca tattatccag gttgtgataa
    56701 atctaaatgt gagattggaa ttattacggg aagcagaaac agcataccac aaaacataaa
    56761 gcaaataatt gacataggtg ataaggtaat tgatataaat acagttatag aaatctttac
    56821 aaaactaaat atatctatag ataaactaca tattattaag gaaaaataca atgaagatac
    56881 agtaagtgga tataggtatt atacaactat gaataaaacc atgaagagtt ttttggagat
    56941 tatagatgaa ggtattgctt ctgttgataa attaggaaaa tttatatatt gcaacaaagt
    57001 attttctaat ctagttggaa tagaccaaaa tgaaattata tctaataact ttatggactt
    57061 atttagtgat aaggttgtca aaaaaatatt ttttcaagaa gatgaagtta atgatgaagt
    57121 agtaaattta aataacaaaa aactgataat taataaagta aatgtttatg aaaataatga
    57181 acgaataaag agtattataa gcattaaaaa tataagtgca attcaagtgc ttgaggacaa
    57241 gattcaaaat aaacttcagt caaaaggttt tgtatcaaag tatacatttg agagtgttgt
    57301 aggtgagagt aaaataataa aagaaaaaat taatattgca agaaaaatag caactacaga
    57361 cttttctgtg ttgatacttg gagaaaatgg aacaggaaaa gaaatatttg cccaggcaat
    57421 acataatgaa tctctaagaa aaaacaagcc atttgttgca gttaatctat cttctttgtc
    57481 agatacactt attgaaagtg aactttttgg ctatgatgaa ggaagtttta caggagcaat
    57541 aaaaggtgga aaaatgggta tatttgaaag agcacataca ggaacaattt ttttagatga
    57601 gataggtgat atatctttag atgtacagca gagattgcta agagtattac aggaaaaaga
    57661 agttatgaga atgggcggaa gtaaaattat accaatagat gttagaatta tagctgcaac
    57721 aaataaagat ttaaagaaaa aaattaaaga aggttctttt agagaagatt tgtattatag
    57781 gattaatgta ttacatatag agatacctag acttagagaa cgtaaggaag atatacctct
    57841 tataagtaag tattttttag atgaaataaa tagcaacaaa tgttttacag aggaatcaat
    57901 gcaagcttta acattgtatg aatggccagg aaatgtaaga gagcttaaaa atttaattta
    57961 ttatattgat actatagttg aggaagatag agtagattac gagcatttgc cagaacaatt
    58021 tagatttgaa aaaaacaata ctttagttaa tgaaaatttc gattcaatta tcttagattt
    58081 caaacaatct aatttctttg aggaaagtat atgtatattg accagtatag aaacttggaa
    58141 taataaaaat atattattag gaagaaataa actgcaagag attttaaaag aaaaagatat
    58201 agttttaagt gtagaccaaa ttagaaaaag aatagataag ttaaagagcc atggtttact
    58261 tttatctgga gtaaaaaaac aaggtagttt tataactgat gaaggaaaga actttatatc
    58321 atatgtaaaa attaagggag tgatataggg gactaaaaag gcaaaacacc ctaaaaaaat
    58381 ccctataatg gatagaaata aataaaatta tctttaagat taagataatt caaaaatatt
    58441 gaataaataa tctaagaaga ttatttttaa taaaacagta gtaaatatca accttactac
    58501 tgtttttatt ttttgcaaga tttattatac aattaaaatt tatattttgg cacatcaatt
    58561 gctacatagg aaaaatataa aaaattgtag aacttggagg atgttgttat gaagtcagct
    58621 tacaaaggat atgaagccta tagttattta gaaaaaggaa aagatatttc atctattgag
    58681 atgtgtaaag gagataaaag agttaaagaa taccttattg aattagatga tattcaagaa
    58741 gctaaggtta aaagtatagt ggatgaaaaa atatttatat cattacatga acatccagta
    58801 ttatttccag aacacctaga aagagatata tttgaatata atagggaagg taaacaaaga
    58861 tgtgcttatg aagcattatc aagaggatat tatgatgcta tatttgataa tttaatggat
    58921 ggtgtatgta cgataacatc aaattcaggg tggaaatggg atgatatatt aatagattta
    58981 ggcatgagac tatgtgattt agctcatcaa gattttgtta taaagtgcga aaaagtagaa
    59041 gatatataca gagctaaaag tgaaggtaaa gtagcattga taccaacatt agaaggtgca
    59101 gcaccaatag aaaatgaact tgatagaata gatatactgt atggatttgg tataaggtta
    59161 atgggtataa catatagcga atccaatgca ttaggttcag gactaaaaga agagaaagat
    59221 ggaggtctta caaatttcgg aaaaaaagct gttgaaagaa tgaataaaat tggaatggca
    59281 atagattgct ctcacgttgg agttcaaact acacttgatg taatagaata tagcaaggac
    59341 ccaataatat taagccatgt tggggcaaga agtttatgga acacaaagag actaactcca
    59401 gatgaagtat taatagcatg tgctaaaaaa ggaggggtta taggtgtaga agcagcacct
    59461 catactacaa taacagagaa gaataaagaa cattcaatag agtcatttat ggaacatttt
    59521 gagtatatta aaaatttagt aggaatagac catgtagctt ttggaccaga tacagtatat
    59581 ggcgaccatg taaaattaca ctcagtacta gctgctaaat ttgatataaa aaaaataaca
    59641 ggaacaaaag aacatcccaa agtagaatat gtaaaaggtc ttgagaaccc aacagaaact
    59701 tcaaaaaaca ttgttagata tttagtaaag catggatata gtgatgagga aatagagaag
    59761 gtactaggtg gaaatatact tagagtacta aaacaggttt ggaaatagat atattagaaa
    59821 tattaataaa atttgaggat ttatagggag gttttataaa tggattattt aaaattagct
    59881 aatcatccac ttttgtggat agcgagtaca gtagcagtat caatagtagt ggtacaatcc
    59941 cttatatttg ctataaaatc ttgcaaagtt ggtaaaacaa tgggaataac agataaacaa
    60001 ataaagtcag ctgttaagag tagtgctatt tcagctattg ggccttcaat gactatattt
    60061 gctggaatgg tatcgcttat agtaactatg ggtgggccta tagcatggat gagattatca
    60121 tttataggtt ctgtaatttt tgaatcaatg tcagccggat ttggaacaga tgcattaggt
    60181 ataactttag gttctcctga aatgacaaaa cttgcattta caaatgcagt ttggactatg
    60241 atattaggtt ctttaggatg gataatattc acattactat ttggacataa actagataag
    60301 ataaccaatg ttatatctag tggcaagaaa tcatttatac caataatatc tctaggagca
    60361 atgttgggtt catttgcata tctaaatgct gatagggtgt tgcgttttga taatggaaca
    60421 attgcatgta taagtggaat ggctataatg gtaatattgt gtatgcttga aaagaagaaa
    60481 aatataaaat ggttaagaga gtgggggctt acaatatcaa tgtttagcgg aatgataata
    60541 gcttcagtaa tataaggggg gagttttatg agtaaagtaa aatctatata caatgaagaa
    60601 tatttacctt ttaagataag gtatggaaga ctaacactat ctttggggat tatagcagca
    60661 ttagttcctg gaataatttt gagctttgga tttggtatta tgcctccaat atcagcatta
    60721 ttagcaagta caatggcaat tgtcagtatg agtgctccaa attacataat agaaccagtt
    60781 tcttattcac caatcttagg tatcccagga acttatatga gttttctttc tggtaatata
    60841 tctaatatga ggctaccatg ttcaatagca gctcaaaagg cagcagaagt tgaatcaggt
    60901 acagaagaag gctcgataat atctacaata ggaatagctg tatctatttt agtgaatata
    60961 agtatactaa ctataggggt aattttagga ggaagcgtac tatctaaaat tccagctgaa
    61021 gtagtagaaa aattaaactt aattttacca gctctatttg ggtcagtttt tggacaagta
    61081 tttttacaag ataaaaaatt aggcctagta gcaattgtaa tatctgtatt gactattata
    61141 ttatcaaagc aaggtataat accacagtct ctagtagttc ttatatgtgt atttggtact
    61201 atgttaattg caagagctat gtataaagat aagcttagtg actaattaca atgaaataat
    61261 agtgaatttt caaatatgaa atataataat ttgattttca aatataaaaa ttacattaag
    61321 attatttaaa aattggtttt taaggaggga atattttgat atatgattta atcttaaaaa
    61381 atggatttat aataggtggt acaggtaatc ctggttttta tggagatata gcaattaaag
    61441 acaacttaat agcaaaaata gattctaaga taaatagcaa tacaaataaa gagattgatt
    61501 gttgtggaaa agttataaca cctggattta ttgaccctca tgttcatgaa gaaatagtag
    61561 caatactaga tggtaaattt gaaaagtttc taaaacaagg agttacaaca actataaatg
    61621 gtaattgtgg acattctata acaccatatt cttctgaaaa tgtatatgaa tatatgtata
    61681 aaaatggtct attacttgaa gaagaaaaaa agtatcttat agataaaaat aaatgttgga
    61741 ataattttac agaatattgt gaccttatat ctaaaagtgg tatttctgtc aatatgggat
    61801 tcttgttggg gcatgggact ataagatgga gtgttatggg aggttctaag gatagacctc
    61861 ctacagaaaa ggaaaaaaat gaaattacag atataataaa tgatggaatg aaaagtggtg
    61921 cttttggaat atctactgga ttggcatata taccaagtaa gtatgctgat attgatgaac
    61981 ttgttgatat tgcaaagcag attaaagagc atgatggtat atatacatct catattagag
    62041 attacatagg aagatataat gcagtaaaag aagccataga ggttgggcaa aaaagtggtg
    62101 ctagggttca agtatctcac ttaagtccag ttgaaataga agcatttgat gagatattaa
    62161 aggctaggta taatggtgtt gaaataatgg ttgacacagt accaagaagt tctgggcatt
    62221 gtatgaagaa aaaaagagta attcaattta ttatggcaat atcatctagt ctatttgaat
    62281 taggtataaa tggggtaatg gatgctctta gaaatgaaga gggaagaaca cttatattaa
    62341 aagaagcatt tatacttgga gataggggga gtataatact tctaaacact aaagatatta
    62401 atatggaaaa gaaatcaata cgtgaaatag ctacacaaaa aggaatagat gaagacaagt
    62461 tattactcga tttactttta gatggagacg aagaacttat cttttgttta ggaggaatgt
    62521 atagagctga tttcccagat aaactacatg atgataaaat aattgataat ccatttgtta
    62581 tggttggttc agattgtttg tttagtgtgg gtggggatat gtcatggttt gaactacaaa
    62641 gaagaggagc ttttccaatt ttcttcaata tgtacagaaa gtctggtgtt agattggaag
    62701 agattgtaag aagagttact tcattaccag ctaggcaatt taaaataaaa aatagaggta
    62761 ttcttaaaga agggttgata gctgatatag cagttattga catggataac tatagttatt
    62821 ctaggtcaga agatattgat ttttcaaagc cacaattact agccaatggt gttgaatatg
    62881 taattgtaaa tggaaaaatt gcgttggaaa atggagagat tactgagaat aaatgtggtg
    62941 aagtattaaa aagataaggt tttaaaaata ataggattta gaaagtaaat aaatatctaa
    63001 gtaagtaaag acgaagtttg agatttattt aaataaaaat catgcttcgt ttttatttat
    63061 agtattaata tttattttaa taatcagata atatttatac tttatagtga aaaaattaat
    63121 atttttaatc attaaattat gtaaagaatt ggataaatag ataactaaat catttgagaa
    63181 aagattttta gaaataaaaa atgataacta aataacccct ttaatattta tcaaatagcc
    63241 agtcataaat attaaaaggg gttatttagt ttataaaaat tatttgtaat aaagtaggat
    63301 atattaataa atatgataga atatatatta taaattaaat ttattaatat aaggggggaa
    63361 ctttgagttc ttatagattt cttacattat aagaacctat actttatgcg ggagttatta
    63421 aaagtaacac ttatagtaat gcttgttttg ataaatgctt tttttgtagc ttgtgaattt
    63481 gctatggtaa aacttcgtag ttctaggatt gataccatga taactgaagg taataataat
    63541 gcaaaaagat gtaaaataat aaaagacaac ttaaattcct atctttctgc atgtcaactt
    63601 ggtataacac tttgttcact tgcattaggt tggatgggag aatctacagt taaagagtta
    63661 attttaccaa tagtaagtgt atttaatctt agtgaaagta ttatatatac tatatcaatt
    63721 tccatttctt ttttaattat aacaatgatg gaggttgtaa taggagagtt ggttcctaaa
    63781 gctttagcac tttataatac agaaaaaata atgttgagta catctttttt attagtagga
    63841 ttttataagt taacatatcc aataatatat tttttcaact taagtactga tttatttctt
    63901 aaaccatttg gatattcaca agctgatgag gttgatgaac cccatacagg tgacgaaata
    63961 agactattag tagaagaaag ttataaaagt ggacttatag atgaatcaga gcaaagatta
    64021 gttgataata tttttgaatt tgaagaaaag aaaattagag agataatggt tcctagaact
    64081 gatatggtat gtatatatga aagtgattct gaagaaaaga tattagctat tttaaaagag
    64141 gaaggtgtta caagatatcc agtatgcaga aaaaataaag atgatatatt aggttttgta
    64201 catataagag atttatacaa tcaaaagata aatgaaaata aaattgaact agaagaaata
    64261 ttgagagata ttatatatat atctgaaaat ttaactatag ataaagctct tgagagaata
    64321 agaaaggaaa aacttcaact tgctatagtt gtggatgaat atggtggtac atctggtgtg
    64381 gtaacaatag aagatatact agaagagata gttggagaaa ttcaagatga atttgataaa
    64441 gaagagactc atattaagaa aataggagca ggttcttact tagttgatgg aactgaatct
    64501 atcaactata ttaataagta ttttggactt gatatagagc atgatggatt tgacagtata
    64561 ggtggatgga tatcctatat gcttggttca aatattaaag taaaccaaag tgtaactttt
    64621 gaaaattaca attttacaat attagaattg gataaattga gggttgtaaa attaatcata
    64681 aaaagtgtaa tataattata tcaaaaaata ttggaggaat tgtatgcaaa aagtagaagg
    64741 aattattttt gacatggatg gagttttgtt tgactcagag agaatttctc ttgaattttg
    64801 gatggagacg tttgaaaagt atggatatac tatgactaag gaaatttata catcagttat
    64861 gggaagaaat cgtaaaggaa taatagaagg tcttacagat atatacgata gttctgtacc
    64921 aataatagat ttatatgacg aaaaaactaa aaatatgatt gaatttatgg agaggaaggg
    64981 agctcctatc aaattaggag tcaatgaact aatctctttt ttaaaggaaa atggatacaa
    65041 aatggctgtt gcaacatcaa caaagagaga aagagcagta aagagattag ctaaggcaaa
    65101 tttaaaagat tactttgatg ctatagtttg tggagatgat gttgtaaact cgaaaccaaa
    65161 ccctgagata ttcctaaaag cagctaaaaa gataaatgtt aatcccaaaa attgtattgt
    65221 catagaggat tcgcctatgg gagttgaagc tgcatacaat ggtggtataa gatgtataaa
    65281 tgtaccagat ttaaaagagc cagatgagca aattaaatca caatcacata agatacttga
    65341 aaatttactg gaagttagag aatacctaaa aagtttgaac tctaaagaat gtcacaatct
    65401 ataatataaa gtattaatta aataaattaa atataatttc tttgatttag aataaatcca
    65461 tgaatatgag aaaaaataag tataaacagc taggaggaat attcatggat aatgtaggtt
    65521 ctctattata ttttttattt aattttttct tgtatggttt tataggttgg ataattgaaa
    65581 acttatatag ttattataaa caagggcatt ttcaagaaga tggattttta aatggtccat
    65641 ttaaaccaat gtatgcaatt gcaatgtcga ttataataga ggtctcaaga tttattgacc
    65701 ccaataatat atttgcattg cttttattat gtctagtagt gccgacagta gtagaatata
    65761 ttacaggaat acttatgaaa gtatatttta ataaggtcta ttgggattat tcagattata
    65821 agtacaatag tagaggaata gtatgtctaa agttttctgt atattggact atattgacat
    65881 ttattggagt tagatatttt caagtgtata tagttgatag tttatatagt cttataatac
    65941 cttattggcc aattgttgta actatacttt tagtaacatt gtttgtggat gaaattctta
    66001 ctataaaatt tttgagggga aataaagata tagtagaaag ataactttaa aaatataagt
    66061 tgaatttaga ttgtagttaa aagaaagcta tgttaaaatg gtgaaatcat ttaaaattag
    66121 ctttcttttt tatttgataa aattatttta taatatatct ataagttgta aagtttttta
    66181 ttaaaattaa aataaagtgt agaaaatggg gagtgattta tgatgggtaa aataagattt
    66241 ggtattgttg ggacaagcaa tatagcaaat atatttctaa gagctgcatc tagggttaaa
    66301 gactttgaat tggttgctgt atattctaga aatttagaaa aagctagaga atttggaact
    66361 ctatatggag caagtatttt ttttgatgat ttagaacaaa tggctaaatc gaaggatata
    66421 gatgctgtat acatagcttc tccaaatgca ttacattcaa agcaaaccat tacttgttta
    66481 aaacacaaaa aacatgtgct ttgtgaaaag tcgttagcat ctaatttaaa agaagtcaaa
    66541 tcaatgataa aaacagccaa agataataat gtattgctta tggaagctat gagaattaca
    66601 tgtgttccaa actttaaagc agttaaagaa aacttgtata aaatagggaa aataagaaga
    66661 ttttttggaa gctattgtca gtattcttca agatatgata aatataaaaa tggaattata
    66721 gaaaatgcct ttaaaaaaga gttgtcaaat ggagctttaa tggatatagg agtgtattgt
    66781 atacatccta tggtgaattt atttggtgct ccaaaaactg taaaggcagt ttctcatata
    66841 cttcaaactg gagtggatgg agaaggttca gctgtatttc aatatgatga tatggatgcg
    66901 ataattcagt attctaaagt agctgattct tatataccat ctgaaataca gggagaagag
    66961 ggaagtataa taattgaaaa gcttaatcta tttgaaaaag ctattattaa atacagagat
    67021 ggaagagaag aagatatatc tattccaaaa gaaaaagcag aggaaaagcc tagagaaata
    67081 gaaggaatgt attatgaact tgtagagttt ataagtctta taaataacaa taaaatagaa
    67141 tcaaatataa attctcatga aaattcaata atagtaatgg aaattatgga tgagataaga
    67201 agacaaagta gtattgttta tccagctgat agtatatagt ttatattaga aaatataaga
    67261 atttataata tatattctta aataaattta attttaagtt aaggtaaggt gtatacttaa
    67321 ccaagtgtta aaatgtcaaa ttataagtat gaaaaagtgc taaaaagaaa tttatttttg
    67381 gcgttttttt cttttttttt acaattttgt aacagtatca aaaaaagttt tgacgatttt
    67441 attacaattt tgtagttgaa tatacatatc aaaactaata tgattaataa taacataaat
    67501 ttgaatattt ttagaggaga gaaaatatga gaaaattgat atactttatt acacctttta
    67561 taataggcgt agtattttta tttgggttag acaaatttct tgattctaaa actgatgaac
    67621 ttttgaggga gaaaaatcta cttccaatta tggatgatac tttaagtgat ataaaagata
    67681 aaggggttac agcaaataat cattttttaa gagaaaagga tattatgatt ttggggtcat
    67741 cagaacttag taattcaaca aaacaacatc caaagtacta ttttaataca aataggagta
    67801 aaaataaggt gtttgcaatt gggagagcct atactcagac attacaagat gctgcaatat
    67861 taggaagtat gaatcctaat attgataata agaaagttgt cttgttaatt tctatgcaat
    67921 ggtttatgga aaaagacgga gtcacaagtc accattatca gtctagattt tcaccaatac
    67981 aattttatcg atttttagat aatccaaaaa tatctaaaca aaataagatt gaatatgcaa
    68041 aaaaatcaag taagttatta tggggaagtg acgagtataa agcagaagca ttatatgcta
    68101 agctatatga gcctaagaca tttcctgaaa aagctgaaaa ggtgctttta gagccttatt
    68161 ttcaaggaag aaagtattgt atagctctta aagaaaaggg tattctttat aaacgattaa
    68221 ttaagctaga taaaaagaga gcaacaaaaa gaaaaagacc aataaattgg tcatatgaga
    68281 gaaagaaagc cattgaagat gctaaaaaaa gagttggtaa aaatccacta aatattgata
    68341 agtactatta taaagagcat tttaaagatg gtatagagca atacaaagga agagacaaag
    68401 atgtaaattt attaacatct aaggagtttg aaaattataa actgatgcta aatgtatgta
    68461 ctgacttagg tattaagcca gttgttgtat taatcccaag tatggataaa ttctataatt
    68521 tgactggtat ttctgaaaaa gaaagaaatc agtactacga taaagcacaa aaaatagcag
    68581 aaagcaaagg ctttgaagtt ctaaacctta aagataaagg gtcagataag tattatttaa
    68641 gggatgtaat gcatcttggt acgaaaggat gggtagatgt ctgtgagaga ttatttaaaa
    68701 tatttaaaga acaataaaga tttgttaact agtatttgtt taactatggt tataatattg
    68761 gcgataggag ttctaacatt tactccattt aaagaattgc catttattta taacggattt
    68821 taaaggagca ttcttatatg aaaattattg aaggaataaa aaaatattca aatacagata
    68881 gaacagcact aatgtgtaat ggagataaac tttcttataa agatttaaat gaatattcag
    68941 atgctatatc agtatttcta aaggatgtat acaaagaaga agatactcct atagtaattt
    69001 atggaaataa agaaaacatg ataatggcat gtatgatagg agctttaaaa tcaggtagag
    69061 cgtatgtgcc tcttgatatt agttttccaa ttgatagagt ttttgaagtc actaaagaga
    69121 taaaaccaaa ggtattattt aactttagtg atgaaagaaa ttttggagat atcaatgtaa
    69181 ttgatatgga taaattaaat tatataataa atgaatatca gggaaaatca ttagataaag
    69241 aaaactgggt aaaagatgat gaaaatgcat atatactatt tacttcagga agtacaggaa
    69301 agccaaaagg ggttcaaata agttcaaaca atttagatag cttttcagat tggataagcc
    69361 catatctaaa catagatgga agtgaaaaag taataatgaa ccaaccagca tattcttttg
    69421 acctttcagt tacaacaata taccctggtc ttatccatgg agctacatta ttttcaatat
    69481 caaaagatgt attagcagac tataaagaac tatttagaca atttagtatc tcagatatag
    69541 ctgtatgggt gtcaactcct tcttttgcag gagtatgtat aactgaaaaa gagtttaata
    69601 gtaaaatgct tcctaacctt gagtcaatga tattcatagg agaagcactt tcaaaaaatc
    69661 ttactaaaga acttatgagt agatttccaa acacaagaat tatcaatgga tatggaccaa
    69721 ccgaggctac tgttggtgta agtgtaaatg atatgactca aaaggctata gatgatgaaa
    69781 aaagtcttcc agttggatac cctatgtcaa attgtaaaat aaaaatactt gatgaagatg
    69841 gaaatgagct taaagaaaat gaaaagggtg aaattataat aattggacca tcagtttcta
    69901 aaggttactt caataataaa gaaaagactg atgaagtatt tttctatgat gaaatagatg
    69961 gtgtaaagtg gagagcttat aaaactggag atatgggata tcttttagat ggaaatatat
    70021 attattgtgg aagaaaagat tttcagatta aattaaatgg atttagaatt gaaatagaag
    70081 atatagaaaa taatttgaga aaagtacata atgtaaaaaa tgcagtagtt ttaccagtat
    70141 ataaggacga aaaaattgct tacttaaaag gtattgttga gttaaatgaa aagaatgatt
    70201 taagtaatat taaaaatggg atgataataa aaaaggaact tggaaagtat ataccttcat
    70261 atatgattcc aagaaatatt tcaataatct ctgagtttcc aacaaatatc aatggtaaaa
    70321 tagacagaaa gaaacttatg gaggaaattt aatgattttt tcacaatatg gagattattt
    70381 ttatctttat atattattat taacatcaat accagcagta attttaggtc taatgggcaa
    70441 gaatataaag tattatggta tgttggcaag tttgtttatg atattcttaa tagtaggaat
    70501 tgatgtccaa ctaaagtact tggtaatatt tattattctt gaagtaataa tagttaaagg
    70561 atatgaatat gtaagaagaa aaactaaaaa taaatacata tattggggat tcttgtttgc
    70621 atcaatgctc ccaataataa taaataaaat atctccagtt acttcttttg gaataattgg
    70681 atttattgga atatcatact taaatttcag aactatacaa atggttattg aaatttatga
    70741 tggagctata aaagaagtta aaatttcaaa gatgctttat tttatgttat tttttccaac
    70801 tttaagttct ggacctattg ataggtcaag aagatttgaa caggacttgg aaaaacaaat
    70861 ctctagaaaa gagtatatag aagaatactt actgccagga ataaaaaata ttgtcatggg
    70921 agtaggttat aagtttgtta tagcattttt gataaataca tattgggtat caagaatacc
    70981 taaagatatg agttttataa atattttaag ttatatgtac gcatatagtt tatacctgtt
    71041 ctttgatttt gcaggatata gtttatttgc tataggtaca gggtacatat ttggaataca
    71101 agtacctata aactttgaca agccatttat cagcaaagat atgaaagagt tctggacaag
    71161 atggcatata agtctttcaa gatggtttgg agattatatt ttttctagat ttgttatgtc
    71221 atcaatgaga aagaaaagat ttaagaaaag gactactgct gctcatgtgg ctcagatgat
    71281 aaccatgata acaatgggat tttggcatgg tctaacttgg tattatgttg cttatggtgt
    71341 ataccaagga ttggcacttg tgttaactga tatatatcag cgaaaatcta aattttataa
    71401 aaaacataaa aaagataaat ggttcgagag agtgcagatt ttcattacat tccatattgt
    71461 gtgttttgga ttgctgatat tctcaggtta tttagcttaa ttttaaggag gaaaatttat
    71521 gcaagaaaca gttatagaaa tatttgaaga tgtattaggg actgatgaga taagagaaga
    71581 tttagacctt aatttatttg agacggaact tttagactca ttagctataa ttgaagtttt
    71641 acttgaaatt gaaaatagat tagggataga gcttcaacca actgatttag agagaaaaga
    71701 tatgtctact gtaaataatt tagtaaaatt cttggagaca agaaaataaa ttagtagtta
    71761 gactttaaat attaatttat aaaataaaat tttacaaagt ccatctgata ctaatatttg
    71821 atggactttt ttaatgataa tacaaagtct tttgaaaata actttaatta agtaaaaaat
    71881 ttaatacata aatatgattt cataaatctt aggtatcatt gtaatattat aggagaaaaa
    71941 tatattatat ttaggaggaa aatgaatgtc tttaaattcg atagatagaa aaaaagaaat
    72001 acttaaatta ctagattcaa aagggaaggt aaacactaaa gaattagtaa aaagattaga
    72061 agtatcatca gaaacaataa gaagatattt ggaagaatta gaaaatgaaa ataaattaaa
    72121 aaaggtatat ggtggtgcta taaaaataaa ttatggagtg attgaaccag aacatataaa
    72181 gagaaacaat actaacctag ataaaaagca aagtattgct aaatatgcag ctaagtttgt
    72241 aaatgacaat gagtcaatta tcatagatga aggtactacc aatttgcaga ttgtagatta
    72301 tataatatct gtaaacaatt taaaaattat tacaaactct tatccagttc tgtcaaaatt
    72361 gatttcttac gagaatcaag gtttgtttga tggagaagtt atatttattg gtggaaaagt
    72421 aaatttaaaa caccaaagga cagcatgtcc aatatcaata gatgctatga aaaacttata
    72481 cgtagataaa gcttttatat ctagtgaagg tatacttgat agttttggaa ttagtagtat
    72541 agaccaaaat aaggcattgt tgtcaaaagc atatataaaa aattcaaagg aaagttttgt
    72601 attatgtgat agttctaaaa taggaattgc tagtatgtat aagatagcag atttagagga
    72661 attagatttt gttatttgtg atgtagaacc tccgaatgaa tggaaggata agttaaatga
    72721 gataaatata acatggatag aagcaaaata aacccttagt tttatataat acaactatgc
    72781 tcaatacaac taaattcata aaatatagtc agttcataga ttaagattag aaagtaaatt
    72841 ttagattgta acttaggatt ttatgggaat atgatacaaa ataaaaacaa ctaaatacaa
    72901 ctaaaaacag aaatttaata tatatttaac ctaaatatga tgtataatta acatatgaaa
    72961 gttatacttt aaaagtaaaa acaattaaaa acacaaaaat tctaaaaaga atctatggag
    73021 gctataaata tgaaaaagat atatggagaa aaaataaaag cagtagtatt cgattgggct
    73081 ggaacaacag tagattatgg atgttttgca cctcttaatg tatttataga gatatttaaa
    73141 agaagaggta tagatgtaac aatggaagaa gcaagaaaac ctatgggaaa attaaaaata
    73201 gaccatataa gagaaatgtg tgaaatggat agaataaaga atatttggtc agataagttt
    73261 ggtaaagtgc caactgaaga tgatgtaaat gaactttatg ctgaatttga gccaatgtta
    73321 tttgagacat tagaagagta tacgactcca attcctcatg ttgttgaaac tattgaaaag
    73381 ttgagaaaaa atggattaaa aataggttct acaacgggtt atacaagaga gatgatgaac
    73441 atagttgaac caaatgcagc aaagaaagga tactcacccg attttcttgt aacaccatca
    73501 gaagtatctc aaggaagacc atatccttgg atgtgctata aaaatgctga agctttaggg
    73561 gtgtctccta tgagttctat ggtaaaagta ggagatacaa taagtgatgt aaaagaaggc
    73621 gtaaatgcag gaatgtggag tgttgctgta ataaaaggtt caagtgagtt agggcttaca
    73681 caagaagaag tagagaacat ggataaggaa gaattaaaag ctaagatgag tatagtttca
    73741 aagaaattta aagaggcagg agcacatttt gtaatagaaa ctatggctga gttagaagat
    73801 atactaataa aaatagagaa tgaaactatt aaatcagatt ttgtaccaga aaatgattac
    73861 atattactta caccaggacc actatctact actaaatcag taagagcatc tatgttaaaa
    73921 gattggtgta cttgggatgt tgaatataat aacctagtac aagatgttag aagaagatta
    73981 gtatctctag caacccaaaa tacagataaa tacacaagtg tattaatgca aggaagtggg
    74041 actttttcag tagaagctat aataggaagt actatttcaa aagatggtaa acttcttgta
    74101 attgcaaatg gagcatacgg aaagagaatg aaagatatat gtaattatct taacatacaa
    74161 tttgttgact gcacttttaa agatatagaa gcagtggatt taaatgtagt agaaaattta
    74221 ttaaaagaaa acaaggatat aacacatata tcaatggttc actgtgaaac tactacaggt
    74281 agattaaatc ctatacaaga ggtagggaaa cttgcaaaag aatataacaa aatttatata
    74341 gttgatgcta tgagtagttt tgggggaatt gagattgatg ttgaagattt taatatagat
    74401 tttttagtta gtagttctaa caaatgtata caaggagtac ctggatttgg ttttataata
    74461 gctaataaag agaaattatc taaatgtaaa ggtatagcta aatcattatc tcttgatgtg
    74521 tatgcgcaat gggagactat ggagaaaaat aatggtaaat ggagatttac ctcacctaca
    74581 catgttgtaa gagcttttta tcaagcttta ttagaattgg aagaagaagg ttctgttgaa
    74641 aaaagatatg ctagatataa agaaaatcag tttacaatag cttcaagatt aaaatcttta
    74701 ggatttgata ctttggtgaa tgataatgct caatcacctg taataacaac tttcttatat
    74761 cctaaaaatg caaagtttga atttatggaa ttttatactt atttaaaaga taatggattt
    74821 gttatctatc caggtaaatt gactgatata gatacattta gaataggtag cataggtgaa
    74881 gtttatccaa ctgatatgga gagactagct gatgtaatag aaaaatttat caatagatag
    74941 aagaagatag tgtcttgtag gttacagaat agtgtggaaa gcccactcct ttaggtgtgt
    75001 gagatgatag cacaaacagt gtataaaaag tattgacagt agaatttaaa gacttatatt
    75061 ttagaagttt atacaatgaa tatataaaga ttgagtaata gggatatatt ttatttaaac
    75121 cacaatctaa gaaattagat ttatagttta aataaagcat ttccctatta tttttatgcc
    75181 tatttataga aaataaatga tagttgcttt attaagtatt attatcatac ttgacaacgt
    75241 aacaatgttt tgttatatta ggtttaaaga taaaatacgg taaagaaagg atatcattat
    75301 gaatgaagaa ttaagttcac taagatttga tggtgttgga aaatgtaaag gtggaatttt
    75361 tgataatgtt aatatatcag gaacaggcaa aatagaagga agtgtaaaat gtagtaaatt
    75421 tgattcttct ggttcagcaa aagtttttgg gacaacagaa tgtaatgaat tttttacatc
    75481 tggtttaagt aaagtagaag gaaacatact ggcatcaaaa gttgagatat cgggacttct
    75541 tagatgtaca ggaaatatta attctgttga aattgatact agtggaatta ttaatgtaga
    75601 aggaagtata aaatctgata ttattcatgg agaaggatat ttaaaagcaa atgagaatat
    75661 agattgtgaa aatatcaatt taagtggaat cttggagtgt aatggatttt tgaattgtga
    75721 agaagttaat attagtttaa atggaacttc taattttaat gaagttggag caagtagtat
    75781 aaatataaaa aaggggcatg gagattattg caaaaaattt cgtatgctag aaaaaaagca
    75841 tatcaacttt gaaaaagtgt cttcaaattt gataagttta ggtctattat caattagaaa
    75901 gtatacagaa agtaaattgt ttgcaaatat tatagaagca gatgaaataa gcttagaaaa
    75961 tagtgaagtc aaagttgtaa gaggaaaaaa tattaaaata ggcaagggat gttatgtaga
    76021 aagtttggag tacagtgaaa gtatagagat tgatgaagat tcaatagttg gtgaaataaa
    76081 gaatataagt gaaaaaataa aattaaagaa ggataagtaa taagtaggtt taaaaataaa
    76141 atttaaaatg aggtaataat atggaaaaag ggtctataaa aatacttgga gaaggcttta
    76201 taagtagtgg tgagtatgaa aatataaaaa tcgtgggaag ctctaaatca aaaggaaatg
    76261 ttaaaagtaa cgaagtaaaa gttcttggag atgcaaaatt tgaaggtgac atcaatataa
    76321 aaaattttgt tgttaacgga aaaactatta taagaggcaa tcttaaagca gagcgtgtaa
    76381 gagtgaatgg tcaacttaat atagagggag atgcagaaat agatgactta gttataactg
    76441 ctcaacagaa agtggatggc aaacttaaat gtaataatgt tacaataaga ggacaattaa
    76501 gtgtaaatga aaatatgtat gcacaagata taaaaatcta tggcgaaatg agaaataaat
    76561 caaatataga atgtgaagat ataaaggtat atggagcaat aaaatgtgat ggtcttttaa
    76621 atggagaaaa tatatatgtt tattcaggag gaggttctta ttgtaaagag ataggggcaa
    76681 ctaatataaa aataggaaga agcaaagatg ctttttcatt aattaaaatc attaatattt
    76741 tttcaggaaa gtttaaatgt aatttgatag agggagatac tattgaatta gaagatgcat
    76801 caataaaaga tatacgaggt aaaagcatat ctctagtaga taattgtgat gttggaaata
    76861 ttgaatacag tgataattta tcagtttcag ataattctac tgtaaaaaac agtataaagg
    76921 tgggctagaa aagttatgga agaattaata tctaagaaag atttattgag caaaactaat
    76981 atatcttatg gtcaattata tagatggaaa agaaaaaaca taataccaga ggaatggttt
    77041 ataaaaaaat cagcttttac aggtcaagag actttctttc caagagataa aatactagaa
    77101 agaatagagt taatattgtc tatgaaagaa gagacttctc ttgatgatat tgcaaatatg
    77161 tttatgaaaa aggatattaa taaagaattt gacattgatt ttattctcag taaagaagtt
    77221 atatgtaatt ataccaaaga aatatttcaa aatttatata aaaaagagca aagcataggt
    77281 aaaaaagaac tgcttatatt gagcataata gagaagtttt tagttaaatc tataataact
    77341 tttgaagagt taaaattgat agtgaatata atagaagaaa attttacatc aatatacaat
    77401 gaaagtggaa agatttattt gtttagaaag tttggagttc cattcgtagt aggatgtctg
    77461 gattataaaa aagtaaagtt tgaaaaagat gtagttaaga ttattgagat tgacttgaca
    77521 aaagaaataa atgatataag tataaagtta tcatagtgta ttatcttata taatgtatta
    77581 taataatata cttaaaatac actttattta aatatttagt ttaaagataa gattttatgt
    77641 gagtatgtaa aagttaaaga tagatttaag taggttatag ggattagtaa acaataaaat
    77701 gaaccatgat gaattactat aaattgtatt taaactacat tttctactta tagtaatatc
    77761 aatggttcat tttattgtta ttaagataat acagtattta taaaaatact tgatataaat
    77821 atagtaaatt gtttaaatag aattattgcc ttaatttatt caaataatga gtttttaaaa
    77881 tctaagaaat atccttgtgg atgaagacat acaggacaag cttttggagc ttctttacct
    77941 atatgtatat ggccacaatg tgtacatatc cattgtatat cttcatttct tctgaataaa
    78001 gtaccttctt ctaaatcagt agcatatcta ccaaatctat caccatgaac tttttctatt
    78061 tcagctatat tttcaaataa cttagctata gattcaaatc cttcttcacg tgctgttttt
    78121 ccaaagtttt tatatacatc atcccactct tgaagctcat tatgttgtgc agctttaagg
    78181 tgttgtgctg tcttgtcata taaatctaca gggtatgctc ctgatatact tatattttca
    78241 ccagcaaatt cttttaattt agaaaagaat tgttttgcat gtgatttttc ttggtcagca
    78301 gtgtaattaa ataaagactc tattatatat aatccttctt tttttgctac agatgcagaa
    78361 atattgtatc tatttctagc ttgactctca cctgcaaaag ctcttaataa attttcttta
    78421 gttttactat cttttaaatt catattctac ctctctatca caataaattt ttacaatatt
    78481 tgattctttt tatttattat tccaatagga gatataatta tgctcaattt agtcagcatt
    78541 tttaaaatta tcattaattt tttttaaaat tctaagaaat tcttgtttct cattttctgt
    78601 aatattatga tatgccgtct ctttaacttg tgttgagatg ctattaaaag tatcttctat
    78661 aagcagtgcc ttttttgtaa gctttatata cgtgatacgt ttgtcatttg tacatttttg
    78721 tctttctaaa tatcctaggt ttataagttt attaaccaac actgtaacag tggatttatc
    78781 ttttcctatt ttagctgcaa tttctttcat tgtaagtatt ccattatttt cataaagtgc
    78841 agttaaaata tttccatgag taggtattaa ctcacctaaa ccatttttac tgagtttatt
    78901 ttcaatgaat ttaatcatac tagcctttgt tttacttatg aaatgtacaa tatatttatc
    78961 ttccataaat tattctccaa tctttaatct atttaaatta gtttgacata aaactattta
    79021 taaactataa ttagtttaac ataaaactaa atgtattggg aggaaattta tgaataaaaa
    79081 atttaaatca ctatatatta cagaagaaga aggaaatttt agaagaaata tagttgaaag
    79141 agaagtggat acattgccag aaggcgaagt tttaataaaa gtaaaatatt cttctttaaa
    79201 ttacaaggat gcactttctg caaatggtaa taagggtgtt acgagaaagt atcctcatac
    79261 accaggaatt gatgcatctg gtatagtaga atctagtagt tgtgatgatt ttaaaattgg
    79321 agaagaagtt ttagtaacag gttatgactt gggaatgaat acttctggtg gtttttcaga
    79381 atacataaga gttccttcta gttgggtagt taaacttcct aaaaatcttt cactaaaaga
    79441 agcaatggat tatggtacag cagggtttac agcagcttta tctgtgtata aatttataga
    79501 ttctgtagac aagaatatgg gagatgtatt agtcactggt ggtactggag gagttgctgt
    79561 aattgcaact aaaatattga ttaaattagg ttattcagtt gtagtttcaa caggaaaatt
    79621 agaagaacaa aaagaagtat tactaaactt aggtgttaaa gatgttatac ataggtcaga
    79681 agtggatgat aactcaggaa gacctctgct tagaccaaga tgggctggag taatagatac
    79741 agtaggagga aatactctag caacagcaat aaagacaact aattattgtg gagcagtaac
    79801 aacttgtggt aatgcaggag gagttgattt tacaagttca gtatatccat ttatcttaaa
    79861 gggaattact ttatatggta tagattctgt ggattgccca atgaacttaa gattaaaaat
    79921 atgggattta ttatcaaatg aatggaaatt agatgattta gatgatatgg taaatgtagt
    79981 atcattggaa gagttaaatg atagtataga ttcaataatg gcaggaaaac atgttggaag
    80041 aacagtagtt gatttagaaa aaatataggt gaataaattt ataattagga ttataggttt
    80101 taagcaaaaa tgagatactt gaaatttact tatgagtatc tcattttttt atgtaaaaag
    80161 atttatgtag atgtttgtgt aaaaagatta ttcatgaaca attaattcaa atacatctgg
    80221 tcttgaatag tgaccttctg aatcaaaatc tagtctacta agtactattt tttctaggtc
    80281 taaatcagct attagcattt cttctttatt gaaaatagga cctgctacat attttccaaa
    80341 agggtcaact atacaacttc ctcctggaca cataatttct ggttctgcat ctagctcctt
    80401 atagtagtta aggtcagttg ggtacgtatt tttttcaaca tattgattgc atccaattac
    80461 aaaacatctt ccttctaaag caatatgttt catagttgct tgccactctt ctcttgaatc
    80521 tgcagtaggg gctatgtaaa gttttacacc cttagcataa agagctgttc tagctaatgg
    80581 catataattt tcccaacaaa tcaacgaacc catttttcca tatggagtat caactactgt
    80641 aagagtactt ccatctcctt ctccccaaat acatctttct gttcctgtag gtttaagttt
    80701 tctgtgttta ccaattagct taccttctgg agagaaaaat aagtttgtgc aataaagggt
    80761 acaatttata tcattaccat ctctttctgt tatacccatt gatagatata ctcctgcttc
    80821 ttgtgctgct tttcctaata aatctgttgt agcactagga actggtacag aattatcata
    80881 gtatcttttc caatcttctc taccttccat tgttctactt ccaacaacaa atccaaatga
    80941 aagacctctt ggatatgcag gtatgaatgc ttcagggaaa actacgatat ttgccccttt
    81001 tctaccagct tctttaatta atcctaatgc tttttcaaca gttttttcta aatccattat
    81061 gactggtgac gcctggacaa cagcaattct tacattttta ttttccttgc tcataaaaaa
    81121 cctcctaaaa aatatttaaa ttttttgcat tgaatagatt acatgagatg ttatttgaga
    81181 actaagatac ttatattgtg aataataatg taactgtata tttaaaataa tacatcatga
    81241 tttaaaaaat tacaattgta cagatataat tgagtttaga tatttaaatt taacaactta
    81301 taatattttt tcaattagta taaagcctat taatttgtct tatttacaat gaatagtatt
    81361 tacttcttgg caaacaataa ttaaaaataa aacttttata tgattaaact ctaaagaaaa
    81421 agtttataaa gaagataata taattaagca gaaaataatg aaaaaaattt attaaagtag
    81481 acaacgtaag gagaatttta tgaaagcaaa aatttattac aatggaaata tcatcacaat
    81541 ggaagattca atatgtggag atgctattct tataaaagac aaaataataa aaaagatagg
    81601 aacaaaagaa gaagtatttg ctttgaaaaa taaagataca gaaattatag atttacaagg
    81661 taagacattg atgccatctt ttatagattc acatagtcac ttgatagctt ttgctactac
    81721 tctaaagcta gttcctttag aagatgcaac cagttttaaa gacatagtaa aaaaaataca
    81781 ggatttcaaa gagtcgaata atataaaaaa aggagactgg ataattggat tttcatatga
    81841 taataatttt ttagaagaaa ataagcatcc agataagagt gtactcgata gtgcatcttc
    81901 agaaaatcca atactaattt cacatgcttc tggtcatatg ggagttgcaa atacattggg
    81961 gttaaaacag cttggtgtta ccaatgaaac acgtgaccca gaaggtggac atataggtag
    82021 agtagaaggc agtgaagaac caaatggata tttagaagaa aatgcatttt ttaatgtagc
    82081 atctaaaata aaacaaccat catcaaatga gatttttaat tcaattgaaa aagcacagaa
    82141 catatatcta agctatggta ttacaacagc tcaagaaggt ttaatggaag aaaatcaatt
    82201 taacatttta aaagcaatgg caaatcaaaa taaattaaaa atggatgttg taggatatgt
    82261 aaatttgaaa aaaagtaagt ctgtagcaga taataatagg gagtttataa aaaaatatat
    82321 aaatagattt aaaataggag gttataagat tttcttagat ggttctccac aaggtaagac
    82381 agcatggttg agtagaccat atgaaaattc agatgatggt tattgtggat atcctatata
    82441 caaagatgaa gaagtagaaa agtttataga tatttcacta aaagaaaaga tgcaactctt
    82501 aactcattgt aatggagatg cagcagcaga ccagttgata gatgcgtttg agaaagtttt
    82561 aaacttaaaa gagcaaagta gtgaaaataa tataagacca gttatgatac atgctcaaac
    82621 agtaagagca gaccagattg atgatatgaa agtaataaat atgatacctt catattttgt
    82681 agctcacaca tattattggg gtgatattca tatcaaaaac cttggagaag atagagcgtt
    82741 taagattagc ccacttaaaa caactataga aaagggatta atttacactc ttcatcagga
    82801 tacaccagta atagcaccta atatgctgga aacagtatgg tgtgctgtca atagaattac
    82861 aaaaaaaggt atacaaatag gagaaaatga aaagataagc ccattagatg ctttaaaggg
    82921 tgttactata aatgcagcat atcaatattt tgaagaagat aaaaaaggtt caattaaaga
    82981 agggaaatta gctaatttga taatattaga tgaaaaccca cttacaatcg accctatgaa
    83041 aataaaagat attaaagtat tgcagactat aagagaagga gaagtattgt attctttgaa
    83101 gtagatgaaa gttagatatt ttataatcta taaaaaatag ttttatataa taagttaggt
    83161 agaattaaat aagattacag ttaaatcaac aattatattt ttattaaatt ttaattattg
    83221 tattgaagat ttataagtat aattttattg attctacctt tttaaataac tgtagattta
    83281 ttaaagttat ctatgtacta tattaaacaa ttaagtagtt attaattgca cttattagtt
    83341 tattaaaaaa ctatgtatat tactttgcag taagactttg gaactaaatc tataatagca
    83401 aatttaaatt tattaaatag attaacttat aacatttcat ataaaatata ttgtgaatct
    83461 gagaaaatta ctatttttct ttgagtattt tttgtaagca gttctaaaag attcaatttc
    83521 atacctatta cttgaatcat tcatcaaaat aattctaatt taaaaaataa ttctatccaa
    83581 ataatagata tataggacta tgttataata taaaaaaatt tatataaaag ttaataacta
    83641 tacaagattg ataaagaaaa agtcaaaata gataattgat tagcaaaata gatataatta
    83701 taatgaagtt aaatctagca atattctcct aaaattaaat ttcatttatt taattaaata
    83761 taatttgaca atgggcaaaa aagtgagtaa aatataaata aaataaaaat aacaggttcg
    83821 gataaagaaa aaggtttctt aacctcaagc ataaagtata ttatctaagt tatttgagat
    83881 taagagataa atccaatctt aggtactgag gaggaaaaaa tgcttgatat aaaaagaata
    83941 agagaaaatt tagattgtat aaaaaaagca atggaaagaa ggggcgaaaa aaactttaac
    84001 cttgatgaag tagttaagtt agatgatgaa agaagaaaga ctcttcaaga agttgaagtt
    84061 atgaaaaatg aattaaatac agcatctaaa aatataccaa atcttataaa agaaggtaaa
    84121 gatgtagaaa atgaaaagat taaattaaaa gagttatctg ataaaattaa ggtaatagac
    84181 caaaatctta aagaagtaga agataagatg gaatacctat taatgagaat accaaatgta
    84241 ccacatccag aagtaccaca aggtgaaact gatgaagata atgttgaagt aagaacttgg
    84301 ggaaagacta ctacctttga ttttgaatct aaggctcatt gggaaattgg aacagaatta
    84361 ggtatacttg attttgaaac agctagtaaa ataactggtt caagatttac tctttacaaa
    84421 ggattaggtg ctagattaga gagagcatta ttaaattttt acttagacac gaatacaaaa
    84481 gtaaatggat atacagaagt tattccacca tttatggcaa atagaaatag ttttttaggt
    84541 acaggacagt tacctaagtt tgaagaagat atgtttaaaa tagaaggtat ggactatttt
    84601 atgattccaa catcagaagt tcctttaact aatatacatg ctaatgaaat acttaaattt
    84661 gaacaattac cgataaatta tacagcttat actccatgtt ttaggtcaga agctggttct
    84721 gctggaagag atacaagagg tttagtaaga cagcatcagt ttaacaaagt agaaatggtt
    84781 aaaatagttg caccagaaga atcttacaat gagttagaaa agcttactaa caatgcggaa
    84841 actatgctac aattattgaa tctgccttat agagtagtaa agatatgtac aggtgattta
    84901 ggatttacgg cttcttttaa atatgatgta gaagtttgga tgccaagtta caataggtat
    84961 gttgagattt catcttgttc aaactgcgaa gatttccaag ctagaagagc aggaataaga
    85021 tttaaaagag ataaagattc taaagctgaa tatgtacaca cattaaatgg ctctgggtta
    85081 gctattggaa gaagtgttgc agctatatta gaaaattatc aacaagaaga tggttctgtg
    85141 gttgttcctg aagttttaag accatatatg ggagtttctg ttattaaata agagttaaat
    85201 tatgtatttt taagtaatac aataatatag aggtaataaa aaataattaa tataaaagtg
    85261 atacaataat atagaagtaa taaaaaaata gttaatataa aagtgataca ataatataga
    85321 agtaataaaa aatagttaat gtaaaagtga tacaataata tagaagtaat aaaaaaatag
    85381 ttaatataaa agcgatacaa taataataga agtaataaaa aaaaataatt aatataaaaa
    85441 tgtgtacaaa ctaatgaata ttgatatagt aagctatcat atgaaagagt atgtacacat
    85501 tttttttact aatttttcac aatatgaaca taaataattt acaggaaagg aatcttttat
    85561 gaaaattcaa aatactgagt ggggatatat tgaatggaaa catacttatg atgaaaataa
    85621 tccaaaacaa gctatgaata tatacattgc agtaactatg cctggtaaga aacattttaa
    85681 tcatgttcat tatggtcaag agcagatgat atatatttta gagggagagg gtttgtatat
    85741 tataaatgga gtttggaagc cattttatca aggtatgatt ttttatatag aatctggtag
    85801 cacacatgaa actataaata caggcgatag agaaataaaa gaacttattg tttcaaataa
    85861 tgtagatgat gtaggagaga gtgaagttat agatattaat cctaataatt atttaaagaa
    85921 gaccttgata aattatagtg aaagtacatt aaatttgtat gcagcagtag aatcaattcg
    85981 tggtcaattt atagacccgt ttaaaattcc attaattata tatgatgatt catggaatat
    86041 agttttaaaa aatccttatt ttccactttt ttgttttgaa aaatgtaatc ctatgaaatt
    86101 tcctcaaaat tgtgattgta tgaatcaaaa aagtagcaat caatttgtat gtgaatatgg
    86161 gataactata tataatatac caatactgta caaaagcaat agcataggtg ttataagagg
    86221 agggtatgta ctattatctg atttgaattt agatacagag cataataatt tatatgatat
    86281 tccagaggga gctgctagaa gtataaaacg cctcttgaaa caaatatcta aaaatataat
    86341 aaatttttgc tcttttaatg atataagaaa ggatttacaa gaaaaagaga agactatagc
    86401 aagaacatat cattatggag agcaacttga aatgaattta aaagtagctc aagatatggt
    86461 tactaattta agaataaatc atcacttttt atttaataca ttaaattcaa tggcaagcat
    86521 agcactagat gatggtagtt atgatttgta tagtgcaata atagatttat caagaatgtt
    86581 tagatataca atgaggtctg atttaagatt tgtagaatta gaatctgaaa tattatacat
    86641 taaaaattat ttgaatttac agaaattacg ttatggagat gcacttaaag taaaatatct
    86701 tattccagag aagttatata acctttctgt gccttttaac tttattcaac ctattgtaga
    86761 gaatgcattt acacatggat ttagagatat tgacacagaa aagcgaattg aaataattgc
    86821 aagattagat tctcagtatg ccattataga gattcataat aatggaacta tcttagatgg
    86881 aaataatata gacaaaataa aggcaggtat tcgtagtaat aatggtcatg gtttgtcttt
    86941 aatatataca aaattcacat ctgcttatgg aaataatttt gatatggata taaagtcgag
    87001 tgataatgaa ggaacttaca taatgattaa aattcctatt gaaaattata aggagtatgt
    87061 atagaacttg tgaaaaatta ataagtttta taaatgttaa gaaatatatt agatgttttt
    87121 atgttgtttt atatatctta cttttttata ttattaagct aaaagggagg tattctcatg
    87181 agaagagcac taataataga tgatgaagaa gctgttctaa aaataataac acattttata
    87241 gaaatgaaag atatgccaat aaaaattgtt ggtaaagcaa catctggtga tgaagcagta
    87301 gataaaatca ttggtttaga gccagacata gtttttatag atattcaaat gccaatatac
    87361 aacgggttag aagtaataga aaaaacatct catttatcta aaaaaataaa ctttgtagta
    87421 ataactgcat ttaactattt tgaatatgct caaaaagctt tgagattaaa tgtaaaagat
    87481 atactcctta aaccattgga tatgaaagaa tttactaagt ctgtagaaaa aattattgga
    87541 tatcaatata ccaataatga tttattaaat gagatactgg aatatataaa tctaaactat
    87601 agcaatgact taaagttaaa tgattgtgct agattatttt taaccaatcc atctaatata
    87661 tctaggattt ttaaaagtaa tttaaataca acttttatat catatctaaa tcatataaga
    87721 atagaaaaat ctattgagtt acttgaaaat actacaaaaa gtataaatga gattgcagaa
    87781 atagttggat ataatagcct aaataacttt tataaaaact ttaagataga aaaaggaatg
    87841 actccaaaag tttataaact gaacactaaa aattgaaatt gataaataaa aagtcaatat
    87901 agataattgt ttgagtgtgt ctgaataagt atactttaat tatgataaaa attaaagcgc
    87961 tttaaacata tttaataata ttaaacctta taaaagctgt acctatttat ataaaaaaat
    88021 atatataaat aaaaagaaaa agatatacaa aataaaaaaa gtgaattaaa tactgtttca
    88081 aaaagacaga attttcattt aatttactga gagtttatat ttcttaaagg aaggtaactt
    88141 tataatggag gtaatttaat atgtcagaca aagtgaatat gaaaaatatt atctgtattg
    88201 caggcgctta tctagcaaca gcaattggtt ctgggtttgc tactggtcaa gaaattctac
    88261 aattttttgc ctcacaaggg tatatgggtt taattggagc attaatatca atggtattgt
    88321 tttcatttct tggtgctgaa gttataacta gaggtagaga attaaaactt aaagaaccaa
    88381 caagaattta cactttttat tgtgggaaga ttttaggaaa attttacgaa tggtttagtc
    88441 caatattttt atttgggatt cttatagtaa tgatttcagg tgcaggctca actttaactg
    88501 aatactatgg tataaatcct tacttaggaa gagtattgat ggctgttgct actttaatta
    88561 cagtgtctat gggtttagaa aaattagtaa gcatacttgg acgtattgga ccagtgatag
    88621 cagtatttac aattgctgtt ggattaataa gcttgtttaa gcattttgac ggtttatcta
    88681 tggttggaga agctatgaaa aatattgaag ttacaaaagc atcagaaagt ccaatcgttt
    88741 ctggtgtaat atataataca ttaaatatta ttgtaatgat tgttttttta acaggaatgg
    88801 gagcttctat tgaaagtaaa aaggatgctt tttggggagg tttattaggt ggaatagtgt
    88861 ttatgactgc tggtatagtt atgtatttgg ctatgatttc agatatagga aatttgtata
    88921 tgaaagaaat accatcactt tatttagcag ataatatatc tccgattgta ggtgtatgtt
    88981 tttcagttgc attaatatta ggtatctata caacagctgt tccactttta tggtctgtaa
    89041 ccaatagaat tgttgaagat gaacatccta aatttaaatt ggtaacaaca gtaatagcta
    89101 ttttggcttg tataggaggt tttttaccgt ttggtaaatt agtaaacatt ttatatccat
    89161 atacaggtta tatggggctt ttaattttag tctgtatggt gtataaagca attacatgta
    89221 gaaaaaaatc aacacaaagt caggaataga gccacttatt aatagttttt atttaaaaaa
    89281 attttttata taatccgaat ttaaaaagtc aaaatagtta aataaattgc aaaacatgat
    89341 aatatattta attaaaataa tatagtaaaa tataaatata aactaaaatt aaatttataa
    89401 aaaatatctt tataaagatt attaacttat ataatttgta gttaaattat aaaaaataaa
    89461 aaaataaaat tttggagagg tgttagtatg catgaactta ttttaatctc agaaaagtat
    89521 aaagaagata ttttagattt ttttacagat attcatatgc accccgaatt aagttttaaa
    89581 gaatttagga ctacaaaagc tataaaagat ttattagtga gtttagacat agaaatatta
    89641 gatttaggaa tggaaacagg cgttgttgga ttattaaaag ggaagtatga tggtcccaca
    89701 gtagcactaa gaggagatat tgatgcgttg ccaatatatg aagaggttga tattaagtat
    89761 aaatccagat atgatggtat aatgcatgca tgtgggcatg atatacacac gtcttgcctt
    89821 gtaggatgtg catatgtatt gtctcatatt agagatagtc ttcatggaaa tgtaaagttt
    89881 atatttcaac cagccgaaga agtcaataaa ggtgcgaaga tgttagtgga acgaggagta
    89941 atggaaaatc caaaagttga tgctatattt ggacttcata atcatccaga tataccttgt
    90001 ggaaaaatag gtgttaagtt aggtgggtta atggcagcag tagacactat taagatagaa
    90061 gtaaatggat ttggaggtca cggaggtata ccaaacagaa caatagaccc tatagtagca
    90121 tcaagtgcaa ttataatggg tattcaaacc atagtaagca gaaacataag tccattagaa
    90181 tcagctgtta tatcaatagg tactataaat ggagggactg caaataatgt aatttcagaa
    90241 aaggtagata tgacaggcac atgtagaagc tttagtaatg aagtacgaaa gaaaatatca
    90301 gaaaatttag aaaatatagt ttgtgagata gctcgtggat atcaagcaac tgcaaagcta
    90361 gattatttat ttgatttacc agctgttata aatagtaaag agatgtacgc aatagcttgt
    90421 aagtctgtgt gtgatttgta ttcagaagat gctatagttg atccaatacc ttctacagga
    90481 ggggaagatt tttctatatt catggaaaaa gccccaggat ttttttactg gttaggggta
    90541 ggcaacaaag aacaagattg tatatatcag tggcataatc ctaaatttaa agctgataaa
    90601 aattctatat tggtaggtac aaatgtacta tgtcagtctg ttataaatta tatggataaa
    90661 ttaaaaaata aaatttaaga tagagcattc tggagataaa gtttatgagg gggtattagt
    90721 ttgaggaaaa ttaattcaca ggaaaaaaac aaagatagtg gattattgat gagggcacta
    90781 aatgttgtag agagagcagg aaataaactt ccagacccag taaccatatt tttattacta
    90841 tgtataattg ttgtaatatt gtcagcagtt atatctaatt taggagtaga agaaatacat
    90901 ccttctacaa aagaagttgt aaaggtagta aatctactag agaaagagca gattcaatca
    90961 tatttaggta gcatagttac aaactttcag tcatttgctc ctttaggatt agtactggtt
    91021 acaatgcttg gagcaggtgt tgctgagaaa tctggattta tggaagtgtt aatgaaaaag
    91081 ggtataagta aagttccaca aaaacttgta actgtagcaa tcgtatttgc aggtatgttg
    91141 tcacatacag cagcagatgt aggatttata atattaccac cacttgcagc acttgtgttt
    91201 ttaggaattg gaagacatcc tcttgtaggt atgtttgcag cttttgcagg tgttgcaggg
    91261 ggatttgcag caaatgttat gcttagtact acagatgtat tgttagctgg atttacaata
    91321 ccagcagctc aaatgatgga cccatcttac caaggcaatc cagcaatgaa tctttatttt
    91381 gcttttatat cagcgatagt tttaactttt gcaggagcat ttgtaactga aaaatttata
    91441 gctcctcgtt ttacaaaata taatgattca acaaatgata aggatataca ggaattgagt
    91501 gaacttgaaa ataagggtat aaaatacgct ttattatcat tgttagttgt agtagtagtg
    91561 atagttgcct tatgtatagg tgacaatgca tttatgaaag atccagagac tggttctata
    91621 ctatctagta atgcaccttt aatgaaaggt atagttccta taatcactat tatattttta
    91681 actccaggat tagtgtatgg aaaagtaagt aagaagataa aaagtgacaa agatttagta
    91741 tctatgatgg gtagtagtat gagtgatatg ggtggatata tagtcctagc atttatagca
    91801 tcccagttta ttaatttatt taatcttagt aacttaggaa caattttatc aataacagga
    91861 gcaaaattac tagcagagtc gggaatacct agctatggat tgataatagg atttatattg
    91921 ctatctggat ttatcaattt atttgtagga agtgcttctg caaaatgggc gattttagca
    91981 cctatatttg ttcccatgtt tatgctacta gactttaatc cagcattaac tcaaatagct
    92041 tatcgtattg gagatgcttc aacaaatcca ataagtccac tttttccgta ttttccagtt
    92101 atattggcct ttgccagaag atatgataaa gatataggta taggtactgt catatcgaat
    92161 atgattccat actcagtggt atttacatta atagaaataa taatattgtt actattcatg
    92221 ggtattggca taccattagg acctggtgga ggaatatctt acgttttata gaccgttata
    92281 ataaatttat caatgaagat taaactaata aaatatattc tttaattaag aggaggatta
    92341 ttatgttaaa aacaacaaaa atttcagacc cagagttata caagattgta gctgatgagt
    92401 tggttagaca agaacacaat attgagatga tagcttctga aagtacagca ccaacagaag
    92461 tacttgaatt aagtggatgt gtatttacaa ataaaacaga agaaggctat ccaggggctc
    92521 gttttcaagc aggttctgaa gaagcagata aacttgaaac cctagcaata aaacgtgcaa
    92581 aagaagtatt tggagcagaa catgtaaacg tacaaccgta ttcaggttct acagctaact
    92641 attgtgtata ttcatcaata ttaaaaccaa atgacactgt tttaagtatg cgtcttgacc
    92701 aaggtggaca cttgacacat ggtagcgcag ttaatttttt acatgacatt tataaatatg
    92761 aattttatgg ggtagaccca aatactggac gtatagatta tgatgctctt gaagcaaagg
    92821 caaaagaatg tagaccaaaa cttataattg caggggcaag ttcatatcca agattaatag
    92881 attatgaaag aatatcaaag gttgcaaaag aagtaggagc ttactttatg gtagatatgg
    92941 cacatgttgc aggattagtc gcagcgaaag taatacctag tcctgtacct tatgcagatt
    93001 ttgtttcttc atctacgaca aagactttct gtggaccaag aagtggtatt gtactttgta
    93061 aagcagaaca tgcaaagaaa ctcgataaag gtgtttttcc aggaacatta gggtctatac
    93121 atcttaatac agttgcagca aaagcctttt cactattata tttaagtaca gataaattta
    93181 aaaagataat ggagcaagtt gtagtaaatg ctcagactct agcttctgaa ctgatttcac
    93241 atggttttag tattgtaagt ggaggtactg ataatcatat agtaatggtt gacttaagaa
    93301 gtaagaatct tacaggaaaa caatttgaaa aagctcttga atatgtagga ataacagtta
    93361 ataaaaatgt tatacctgac gacccacaat caccatttgt aactagtggt gttcgtatag
    93421 gtcttacatc aatatctcaa agaggtctga aagaaaaaga agttatacag atagcaggga
    93481 taatgaataa ggtagcagaa aatattgata ataaagaagt attagatgaa tgtaaagcag
    93541 aggcacaaga acttatatct aagtttccat tatatccaga agggtatttt gaagattaaa
    93601 tattttgatt tgtatagctc acaatttata aagcctgtaa cataaaattt aataatatat
    93661 aaccactata aggtgttgat taatcaacac cttatttttt ttgaaaagta atatacaagc
    93721 tatcataagc atataattcc ttatagataa ttaacaaggg ggataaagtt atgaggtatt
    93781 ataataaacc taccaaagaa gtattaaaat acttaaaaac aaatccagaa attggacttg
    93841 atgataatga ggtagaagaa aggaagctta gatatggatt aaatgaattt actataaaag
    93901 aaggtagaac attttgggac gagttgggag aaagtttgac agaacctatg atattaattc
    93961 tcataggagc agcagtaata agttcttttg tgggagaatt acatgacgca ttaggtattt
    94021 taggggctat ttttattgga atttctatag gcataataac tgaaggaaaa tctaaaaaag
    94081 cagctcatgc cctgtcaaaa ttaacagaaa atatagaagt aaaggtgctt agaaatggga
    94141 agataattaa aatatcaaaa aatgatttag taccaggaga tattgtatat atagaaactg
    94201 gggatatgat accagcagat ggaagactta tacagtctat aaatttgaaa cttagagaag
    94261 atatgctaac aggagaatct gatgatgtag ctaagaatgc tgatgctgta ttagatatgg
    94321 aagtagttta ttctaaaaca gaaataatcg aacaggatgc aatacctgca aagcaagtca
    94381 atatggtatt tggagggact cttgtagcat atggtagggg cataatggtt gtcactcata
    94441 ctggtgataa gacagagatg ggtaagatag cacagaattt atcaaatgaa gaccagcaaa
    94501 ctccacttca aattaaatta gggaagttgg gtgctaaaat agcgggaata tcaggcgtaa
    94561 tagctacttt attatgtatg tttatgatta tacagatgca gagaaaaggt atgttaattt
    94621 tagatacaag cagtgtatta tctttcttac aatcattaga acctgctaaa aatgcatata
    94681 tggtttgtat tgcattaata gttgcaactg ttccagaagg tttgccaact atgataaata
    94741 ttaccttagc tataacaatg caaaagatgg caaagataaa tgcattagta acaaagaaag
    94801 aagcctgtga aactataggt tctgtttctg taatatgttc cgacaaaaca gggactttaa
    94861 cacaaaacaa aatgatggta gaggtagcat atgttgatgg taaatatata agtggaggag
    94921 agtatcaaag taatagctat tttgaacaaa actgtatagt aaattcaact gcagatatag
    94981 aaaaagaaga taattcattt aaatatatag gaagcgcaac agagtgtgca ttacttttat
    95041 atcataatga taagaactat aatgaaatga gaaaacaaac gtatttgata tcacaaatac
    95101 catttagctc agaagaaaag aagatgtcaa cattaattcg tcaagaggat tcagatatat
    95161 tactttcaaa aggtgctcca gaagtattat taaaaaaatg ttcttatgta caacaaggaa
    95221 aaaatattgt tccaataact ccaaaagtag aaaaaagtat tttagatgag attaagaagt
    95281 tacaaattaa atctatgaga acattaggat ttgcttataa aaaaatgagt aattctaaaa
    95341 cagaagttgc aatgacttca gaaggtgaat tgaatttaat aggaaattca agaagttata
    95401 tgaaggaaga taatttagtt tttagtggat ttgtaggaat agtggaccca ttgagagaag
    95461 gggtcaaaga ttccattgat aaagcattta atgcaggagt agatgtaaaa atgcttacag
    95521 gagataatat aaatacagca acagctattg gaaatgaatt aggattatta aatgatggga
    95581 aaaaggcagt tgaagcaaca tatatagatg tattgacaga taaagaattg agagaagaaa
    95641 tcaaaggaat atctattgtt gcaagaagta aaccagatac aaaaatgaga atagtttcag
    95701 cacttcaaaa aagtggcgaa gttgtagctg taacaggtga tggtataaat gatgccccag
    95761 cacttagtca agcagatgtt ggaattgcaa tgggtatttc aggaacagaa gtatctaaaa
    95821 atgcagcaga tataatattg actgatgata gctttagtac tatagtagag ggaataaaat
    95881 ggggaagagg tatatatgag aactttcaaa gatttataca gtttcagctt actgtaaata
    95941 tagtagcatt tataattgct ataatatctc aacttactgg taaggatatg ccatttacta
    96001 caatacaatt attatgggta aatataataa tggatggtcc tccagcccta gcattaggat
    96061 tagaacctgt tagagattat gtattgaaaa gaaaaccaat aaatagacat tctggaataa
    96121 tagctaggtc tatgtttgta aatattatta taaatgctat tcttataatt acaattgtat
    96181 ttactcaatc agcttttaat atattgggag ctacatcaga agaacaaggt actgttatat
    96241 tttctttatt tgcattcagt gcccttttta atgcattaaa ttgtagagag tttggtttaa
    96301 atagtacaat accaaatttc tttaaaaata aactggcatt gcaaataata gtagttacag
    96361 ggataattca aataatattt acacaagtgt tccagagttt ctttaattct gtttctttgg
    96421 actttgatat gtggataaag ataatactat ttgcatccac tatcctattg tcaaatgaat
    96481 ttgtaaagct aattcttaga acaatgaaaa atagtagaac tatgaacttt aatagtaaaa
    96541 attaattcct atgaaaccgc aaagtactat atattgtaac tttgcggttt ttgtttgttt
    96601 aatgttgttt tatattacca ttttcatatt taatttttta ttaatctaaa cacaatcttc
    96661 cattgtaatt gcttcatctg tgtcaacaca ttcaaagtca atatctaatt tttgtaatat
    96721 tttgtatgcc tctttttgca aaggagtatc aaattcaata ggtgattttg tgtaaatata
    96781 actgataaaa aacatgattt atttcattcc tttccattta aaaacttata gttatagtgt
    96841 atcataaggt tataaaatat agataaatat agtataaatt taaaatatgt acagacaaaa
    96901 caaaaaaatc atattctaag taatacttgg gaaaaatgat attatcataa taaataatct
    96961 ttgtaaaaac ttatatagaa tctattaaac agacactaag ccataaaaac tttactaaat
    97021 ataaaattcg tatctaaata gatatataga ataatatttt actgaagtag agtgattttt
    97081 aagaatttta tatataaatt atgttatcat aaataaagat atagattata aaatttgtta
    97141 ataaaataaa gggcagggag gtaagtttca tgttttccaa aatcctagtc aaaacattta
    97201 tcagagatag cgaaaacgtt caaaatacag atgttagaaa taagtatggt tatgtagctg
    97261 gtgtagtagg tatattatcc aatttacttt tatttgtgat aaaggtattc ataggaatgc
    97321 tcacatctag tatagctata atggctgatg catttaataa tctttctgat atggcatctt
    97381 cagcaataac tatgataggt tttaaattag caagtaagcc agctgataaa gaacaccctt
    97441 ttggtcatgg aagaatagag tatttatcag cattaatagt agcatttatg gtgatgttgg
    97501 ttggacttca atttgtaaag tcatctatag aaaggatagt aaatccaatt cctgtaaaat
    97561 ttgaagtaat accacttata cttttaattg catctataat gattaaaatc tggttgagtc
    97621 gttttaataa atttatggga aataaaattg attcttcagc cttaaaagca gtttcacttg
    97681 atgctttagg agatgtattt acttcaagct gtgtagttat atcatttatt gtagctagat
    97741 ttactaattt tcctatagat ggatatgttg gtatcgtagt atcattagtg atactttatg
    97801 ctggtttctc tcttgtaaaa gatactataa atcctctttt aggggaagca ccagatgaag
    97861 aaatggtaaa ttctattata gagctattgt tatcttataa atatataata ggtacacatg
    97921 atttaattat tcataactat ggagttggta gatgtattgc atctatacat gcagaaattc
    97981 catccaacat agatataatg gaaatacatg aaattataga tactgcagag agagaaatat
    98041 ctgaaaaatt agatatatat ctagtaattc acatggaccc aatatgtctt gaagataaag
    98101 aagttatgtc agccaaaaaa gaacttgaag aaatacttaa gaaaaatagt cttgtaaaat
    98161 ctatgcatga ttttagaata gttggaaaag gaagcaagaa gaatttaata tttgatatag
    98221 tagttaatcc ttccgaattt tccaaaaaca tgtcagaaga tgacttaaaa gaagatataa
    98281 caaaattagt taaggagata aatcctgaat ataattgtgt aattgttgta gataaggatt
    98341 ttatttaatt attaattaat ctcatcataa ggtaaaaaat gtaaaaagga tagacaatag
    98401 tatagaggca taatataata gaagtatata attgtatttt taatataaat aaagcaattt
    98461 taattgtttt tatgaaatat tatagagatg ttgaagtata ttctattatt gggcacctta
    98521 tggatatact gagtcagtgg tgcaaccggc tatgaatata aatttattta ttttcatagc
    98581 tttttttgta taatttttta gtaaaaatta ctttatatac gatattaatg gaagattaga
    98641 ttataaatta taggtaacct aaaggggtga gatttacatg aagaaaggaa atagaaaggc
    98701 attattaatt agtttaatta tgattttaag tatggtggtg tctacaatat atccaactgt
    98761 atcttatgct tcagaattag gagagaatag tcagattcaa agtggttcaa ctaattcatc
    98821 aactggagag gagaaggaaa gcgacaataa aaagccagaa cagactccag agaaagataa
    98881 ggcaacagat aataaaaaac cagaacagac cccagaggaa gaaaaaccaa cagataataa
    98941 aaaaccagaa cagaccccag aagaagataa gtcaacagat aataaaaagc cagagcaaac
    99001 tccagaagga gaaaagccaa tagataataa aaagccagag cagataccag aagaagataa
    99061 gtcaacagat aataaaaagt cagaacaagc tttagaagat gaaaaaccac tagacaataa
    99121 aaatacagaa aaaactccag aggaagataa tctattagaa gatgaaaatt tattgaaagt
    99181 tttagaagaa gaacttaatg aagaaaatga agactatgga tttgttgtaa aaattaataa
    99241 caatacaata gaaacggaat caatgaaaaa aatatcattt aatttaactt atactccaac
    99301 atcaaaagga atacaagcag gagattcaat aacttttaag gtgccagatg tattcaataa
    99361 agttaattta gattatacaa gtgaatgctt tgataaaaca gaaagtaatg gagagtatac
    99421 acttacattt agagaattac ctaatggcca aagtgtaatg caaggtaaaa taggtttgga
    99481 agcatatgtt aaaaaggttg atgaagatac taatgcaaaa atacatattg aaactactgg
    99541 aaaaatagaa agtggaagtg gagatataga tgtagaaata aaacctggag ataaaacgga
    99601 tgttccagat gctaaaggaa cacttaaaaa gcttgttgaa ggaaaaaaat caactactgt
    99661 atttatgcca gttaaaaata aagatataaa ttacagtata caagtaaatg aaaagcagga
    99721 agagttaaaa gatattattt tgtatgatga gcttcctgaa ggattgacat tgataaatgg
    99781 ttctgtaagt gtagtaacaa gtgatggaaa agaagttagt gattttaata ttgaacagag
    99841 taaaaattct atttcagtta attttggaaa tatagataaa tcgtatactg ttaagtataa
    99901 ggctagaatc agtgacaaaa acgcaaaaca tggtaataaa tataagaatg tagctaggat
    99961 tgaatctgat ggtaagaaga tacaagaaga tgatgctact gtaagtatat ttgatagagg
   100021 agatgattat cttcttacta aaggacattc aggagcaaca aatattactc aagttggtca
   100081 agtaataaac tatcagattt caataaatga tgataaatca ccaataagta atgttgttat
   100141 aacagataat ataccagaag gcatgagatt aactacttca ggagaagctg ggcatgattt
   100201 tagagttgta gaaattccaa tgaatgggag ctggactcct tggagtaaag aaaaaattgc
   100261 caataatatt tcatataaag tagaagaaaa aagaaatgaa agtgggcaag tagataaagt
   100321 tataactgga tttacaatta atctcagtaa agaagaagtg gaatcaaagt tttttatagc
   100381 ttatacatta aaggtaatta gtattgaaga tagctatata aatagagctg ttcttgatgc
   100441 aaataatagt gaaattgata aaaatgatga gataaacttt aagaaaaatt ctggattaat
   100501 aagcgcaaaa aaagaagttg ataaaaaagt tttaaatagt agtgataacc agattgttaa
   100561 atacaaaatc aatatgtcaa cttatggagt ttatgatgca ggacaagtaa atcttttaga
   100621 tgaagtaaac tcagctctag aaatatctaa tataaaatat tctgataatc ttgaacttaa
   100681 aaaagaagct ggagatggaa aaaatacaat aagattagta aataaatacg aatttaaaca
   100741 gataaaagaa ggggaacctg tgcaaagttg ggttacattt gatgcagact tcacaaatgt
   100801 aaaagttggg gagactatac aaaatgtggc tcagattaat ggttcaagtc ctccaggagt
   100861 agagactact aagcaaggtt atgcttttga agctaaaaaa gtggatgctt tagataaaaa
   100921 tgtactttca ggtgctaagt tcaatttaga agatgcattt ggaaatatag tagttaagga
   100981 tttagttagt gatgaagatg gtataattca atctagtata aaaaaaccag gaacatatta
   101041 tttagtggag ataatggctc caagtggata tgaaaaactg aaggataaaa ttaaggttga
   101101 aattggtaat aaagatattg gtagaatagt ggatattgga aatatagaaa acataagaca
   101161 agaaaatcca ccagtaaatc caccagcacc accaaataca gatgaaccaa tagtaaatcc
   101221 accagtacca ccaagcacag ataaaccaag gaaaccatca agttcatctg atacagagga
   101281 tactatagtt ataaatccac caataccacc atcagaagat ataataaatc cgccaatacc
   101341 agaggtattg aatccgccag taccaccatc agaagagatg atagaaactc ctgttaaaca
   101401 gattattcca ataccagaag tagtaaagcc atcagtgtca gaagaaaaaa ataataaagt
   101461 taaagatgat actttggtaa atccaccagt accaccaaaa acaggggata gtactacaat
   101521 tattggagaa atacttttgg ttataggagc tattgtagga cttatcgtat taagaagaaa
   101581 taaaaataca aattagaccc taacattata aaaaatataa gttatattaa aaaatatctt
   101641 ctaaaacaat atttaataat gaaaatattg ttagaagata tttttttttg aagatttatg
   101701 taaaaaaata ataacttttt agaaaaaagt tccaaaggga tatagacaat taaaaagata
   101761 taagctataa tatagatata aaattaaatg aggcaaaact agggaaacct agtcacgcaa
   101821 agctataggg gctaaagttt agtaaaccat gccagccagt tgccgaaaag agtaaaaatt
   101881 tctttttgta tatacaaaga tactcttggg tatctttttt attttgaaat ttattactcc
   101941 cggcctaact caaaggaggg aataatatga gaccaagtaa aaaattatta atagctataa
   102001 tatcaatatt cttaataagt tcagttccag taagtgcaca tgcagatagt actactatac
   102061 aacaaaataa agacacactt agtcaaatag tagtttttcc aactggaaat tacgataaaa
   102121 acgaagctaa tgctatggta aatagattag ctaatataga tggaaagtat ttaaatgcat
   102181 taaagcaaaa taacttaaaa ataaaattat taagtggaaa gttaacagat gaaaaagaat
   102241 atgcttattt aaaaggtgta gttcctaagg gatgggaagg tactggaaaa acttgggatg
   102301 atgttccagg tttaggtgga agtacagtag ctcttagaat aggatttagt aacaaaggta
   102361 aagggcatga tgcaataaat ttagaattac atgaaacagc acatgcaata gaccatatag
   102421 tattaaatga tatatctaaa tcagcacaat tcaaacaaat atttgctaaa gaaggtcgtt
   102481 ctttaggtaa tgtaaactac ttaggtgtat atgcagaaga attttttgca gaatcatttg
   102541 catattacta tttaaatcaa gatacaaata gtaagttaaa atcagcttgt ccacaaactt
   102601 atagcttttt gcaaaattta gctaaataga gttttaataa atttaaaata aatataaata
   102661 aagagatgct attataatag catctctttt atattgcgtt taaatctaat aaattttgtc
   102721 ttatgtataa ctatgataga ttatcaatta ctactcctta tacaaggctt caacgtgttt
   102781 attatgcatt tgagtaatta tcgtaggtat aatagaaatt attgaaccaa ttactatcaa
   102841 taaaattcct ataaccatgt ttatagggat aatttcttta agtattaata atgctagtat
   102901 tggagatatt actggtttaa agaagaatac taaagatgta agactagtag atgtagcttc
   102961 cattgccata aagtaaagcg cataaccaac acctgtaaca aatacgaata cataaatcat
   103021 aatgggcata ttgtgtaagg tatatccact aaaaataggg atatttgcaa atacatgaag
   103081 cttattttta tttaatatat ctgcgataaa attaatttta gtgagtaaaa ccaatatcaa
   103141 catttccaaa ctaccaaata taaagctaaa gcaggttaca actattccac caaattttaa
   103201 ggtttttctt ttaccaaata cagtatacat tgaaaaaata atagcagaag ataatgttaa
   103261 gaatattcca ctaagagtta gttttgtatg caatggattt attataacta taattccaag
   103321 aacttctaat ataagtgaaa ttacattgtg cctatatatt ttttccttca aaattaagta
   103381 agctaaaagc attacaaaga ctggattaca actgaacaaa acagcaacta cagatgcctt
   103441 tgtgtttaga acagcaagtt gatacaatga catacttact actacacaca taaaacctaa
   103501 aaatgcaaag tattttaagt ctgattttgt aatagaaaga ctaagagctc ttaatctttt
   103561 tacagcaaaa ggtagtaaga ctaaaccgcc aacaaaaaat cttgataaag ttatttgtaa
   103621 gggattaaag tcattagtga cagtttttag actaatttcc attgaactaa aaaataaggt
   103681 agtcagtatt atataaacat aacctttttt caagtcaaac acctccttgt aatttatatt
   103741 agtactatag ataagaatat tcaagcatat tggaataata tggtatgtat aaaatatagt
   103801 acataaaatt ataaactacc ttaatataga atgtaagtat tctatactaa ggtagttaat
   103861 tatttgaatt actttgattt agttatttta aattatataa tttggctaat ttttcaaatg
   103921 ctggtaaaga acttttaact ttatcataag atacacaata tgatactcta aaatgacctg
   103981 gacaaccaaa agaagaacct ggtactaata ataaattaaa ttgttttgca tcattgcaga
   104041 attttttatc atcttcaatt ggagcttttg ggaacaaata gaatgtacca ttaggtttaa
   104101 cacaagaata tcccatatcg attaaactat tatatagaag gtcaaaattc tttttataaa
   104161 tatttacatc aacttctgca tctaaacttc ttgcaataac cctttgaaaa agagaaggag
   104221 catttacaaa tccaagtatt ctatttgcaa catttaatga agacataaca tcatcaaaat
   104281 catccatttg cccattagca acaatatatc ctatacgttc accaggtaga gataatgatt
   104341 tactataaga gtatcctata aatgaatttc tatagtattt tagtacacaa ggaactttgg
   104401 caccatcata aattatttct ctatatggct catcagaaat taggtatatg cttgtattaa
   104461 attctttttg ttttgaatcc aataattctc caagattttt taataattct tcagaatata
   104521 tgacaccagt aggattattt ggagtgttta ttattaaagc tcttgttttt ggagtaatag
   104581 cattttttag agcatctaaa tctggctcaa aagtctctat atttgtagga acttctatta
   104641 attttccatc atagttttct gtataatttt tatactcacc aaaatacggt gcaaaagcaa
   104701 taacttcatc accaggattt aaaagagttt ttaaaataat atttagacca ccagcagcac
   104761 cacaagtcat aattaaattt tctcttgtaa gatttaatcc atctttttta tttatgtgtt
   104821 cagcaatagc atctcttaca tcctcatatc ctgagttgtt catatatcca tgaactaaat
   104881 ttggagattc ttcatttaaa atatcattta taactgcttt aatagtctct ggaggttcaa
   104941 cacttggatt tcctatacta aaatcaaaaa cattttcctc accataaata tcagaaagtt
   105001 tctttccttc ttcaaacata gctcttataa cagaactatt tgcaactaaa gtttgcattt
   105061 tattagatat catatgtaac actccttttt atagatttat attgcttatg tatatattac
   105121 catattttta ctgaatttag acaattttcg aaaaattact tgtttgaaaa tataaacttt
   105181 tatataaaat atatttatgt acagtacata ttaaaaattc caatttctaa ttttatcatg
   105241 attattttat ataatagttt tttaaagaat taataaaaca tagatttaga gaataaaata
   105301 ttcttgacta tattaattgt ttaatataat atagttgata tagtcaaaca tattataatt
   105361 atagaaggga aatttaagat ggattttaaa aatttacagt atgaaagttt gtcatatatt
   105421 atttgtaact tgcaaaaaaa ctttaaactt tattgtgaaa aatgtttaaa accatataaa
   105481 cttacgaatg gtctatattt ctatttaatt tatattaata aaaataggaa ttgttctttg
   105541 aatgatgttt ctacagaatt tgaagttgat aaagctcata caacaagaac tatatctagg
   105601 ttagaacaag atggatacat agaaaaaatc caaaacccta atgatagtag agcttttcag
   105661 ttgagagtaa cagataaggg tgaggaagta ttaggggata taaaaaacat attttcaaag
   105721 tgggataatc acataaaaaa agagttctcg gatacagaat ataaggaact ggtaaagaat
   105781 ttacatgtgg taaaggatat aaaaacagct gtggaggaag aataggatgt attgtaattt
   105841 attaaaacca attaaaattg gaaatttgga attaaaaaat agagtatctt ttgcacctac
   105901 aagcatgggt cttaaattgg aagaaaaaat taaaaagttt tctgatattg ctaagtcagg
   105961 agtagctctg attactctag gagatgtatc tattagacct agttttcata aggtagctat
   106021 aagcctatcg gatgaagatg gagtcatgaa atacaagaaa attgtagatg aaatacacaa
   106081 ttctggtgca aaggtatcag cacaactatt ttgttcagat tatgatgtta atttaattaa
   106141 agatactatg aatatgggaa ttacttcaca tgatgagata aaaaaaataa tgaatgacgg
   106201 agtaaaagat tacataacaa atatgcccaa agaagaaatt aaaaatatta taaatttatt
   106261 taaagtaaca gcattgaatg caaaaaaagc aggttttgac atgattcaaa ttcatggtga
   106321 taggcttgta ggaagttttt catcaagtat atttaataat agaaatgatg aatatggtag
   106381 aacgtgtgat aatagaagta gatttgcaag cgaaataatt agtagtatcc gagatgaagt
   106441 taaggacatt ccaatagact ataaatttgc aataagacaa gaaaatcctc attatggaaa
   106501 tgcaggagtt ttactttcgg aagttgagta ttttgttaaa aaatttgaat ccctaggtgt
   106561 aaacagtttt catgtaacac ttgcaaatca ttcaaaatta gaagatacta taccaaccaa
   106621 taatcatcct tactttaaag aagaaggctg ttttttatat ttagctgatg aagtaaagaa
   106681 acacacaaat cttccagtat gtggggttgg aaaattgtct agcccagatt ttatagaaag
   106741 tattatttca aataatagag tagatatggt ttctatgtca agacaattac tagcagatag
   106801 gaattggtta cagaaagtaa aagatggaag agtagatgag attaaaaagt gctgttattg
   106861 taataagaag tgtgcagatg ctttacaaac acgctctcag tttggatgta tactagatta
   106921 gaatatcctt ataaaaataa ataatttgtt aaggttaata tatttttggt gatttagata
   106981 agtatttaga taaagaggag gagacatcat gaaaagttta gttttatatt caagtttaac
   107041 tggaaatact aaaaagattg cttatactat atatgatgaa attcaagaag aaaaagacat
   107101 taaagatgta aatgaattag ttgattatag tattgactat gaaaattatg atatagtatt
   107161 cttaggctat tgggtggaca agggaatttg tgataaaaat tcaaaacaag tgcttgaaaa
   107221 tattcacaat aaaaagatag cactatttgg aacaatggga gcaagtaaaa aaggaagtta
   107281 tggagctagt ataatagaaa agatagaatc aattatccca aaagataatg aaatattagg
   107341 ttcattcata tgtcaaggta agatagcaga aggattgaaa gcaaaatata aagagatgtt
   107401 aaaactttct cctgacaacg aacatattcg ccaacaatta aataaccatg aggaatctca
   107461 aagtcatcca gatgaacaag aaatatatga agccagtatg tttgcaaaaa atatgatgat
   107521 taaagctagt attgtgtagt agttaaacaa taagttcata taagatatag aataaataat
   107581 ttagtaaaaa agtatagcaa ataatttgct atgctttttt tatgaaatca aaaaaataat
   107641 aaaaatactt gactgtaaac cttgtttata cattatttta tagaaaataa gatagtttta
   107701 aatataaaaa aagatatatt attgaaagga gatattattg tatgaaaact tataaaagat
   107761 aattagtttg attttaagat atactaaata tacaatttta tagagtgaaa atatttaaga
   107821 ctatattata gttataaatt aatagaatag agaggtagtg tttaaattgc aataacagta
   107881 tttccaaagt tgccacctca aattcctatc caatggaata atggtgtagc taattatgta
   107941 gataaaaaat ttatttatgc ctaccctgtc atttgtgttg tattcagaat ccttttacgt
   108001 ccatttatat ggagatggtt acaaatacat gctttttata gtgacactat tgcagattat
   108061 ttaacgaatt ttatgtgttt tgtagcgttg tctatagaaa tattctctat actttttatt
   108121 tacaatattg ccagacatgt tacaataatt atttttatag atatacttgt atttattgct
   108181 ttactgataa taggtatgta taaattattt aaaaaataca attaattttt aaaaattaag
   108241 taataagtga tagtgtaaaa agtagccact gcaaattatg gattgtagtg gctattttat
   108301 tatttgtata tgaaattatt ttgtttttct aataagttac gatatattat ggtttatttc
   108361 actgataaaa ggtttaggta ttttaaagta aaaagttgta tattctaata aaatttaaac
   108421 attttgattc tttaaaatta gagaaaatac attgttctta ttaaaaaatt aaatattaag
   108481 aatttaattt ctgcatctta tttagaaaag ataagaaatc aactttatat atttttttgg
   108541 gccttcctct ttgattatcg agttttgtat ttacaatagt tgcaattcct gctttatata
   108601 agatactcaa tattctatta gcacttctaa gagttatgtt catatagttt
//
LOCUS       DAEPIN010000004       108605 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.004, whole genome shotgun sequence.
ACCESSION   DAEPIN010000004 DAEPIN010000000
VERSION     DAEPIN010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 108605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 108605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..108605
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.004"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            347..1378
                     /locus_tag="KR250_000332"
     CDS             347..1378
                     /locus_tag="KR250_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HBH0066303.1"
                     /translation="MENICDMNNKVKVAVLDTGIDKEHDYLKDNLVGGIAFECMHDYI
                     FISDKFDDEDGHGTACASIIKKEYEDVELFVIKILRKDGITNIKVLEEALRYLLDTNI
                     RLINLSLSVIGVESVKGLFEVCYELFRKGKIIVCSLANDFDLSYPAMFNNVIGVRGFT
                     LDGDDSFWYNKKYDVQCVMDSNSYISCDINNSYRLPPKCNSYLSAKLTGKIAKILSEK
                     LGITISDLNDKLESLATRNHWDSCDFDECSRIVNFKLDLYDKENALFMKVADVVRDCL
                     DIEENNEKLFKGSLFNKEIGLVYDNCFNLLKKLEHRFDIKFNYMDISKYDLVSIYTLT
                     ELVERYKNL"
     gene            1511..2809
                     /locus_tag="KR250_000333"
     CDS             1511..2809
                     /locus_tag="KR250_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HBH0066304.1"
                     /translation="MKYEEALEYISQTNKFGIRLGLENIGKLLELLGNPQETLNIIHV
                     AGTNGKGSVCSFVSNILRECGYKVGLYTSPYLETFTERIRVNGQNIPQDDVARIIELI
                     KEKIEIMVKEGYAYPTEFEVVTAMAFYYYSEQKVDFVALEVGLGGRYDATNIITKSLV
                     SVIVSISLDHTGILGDTIEKIAYEKAGIIKENGVVLVYDQTDEAKDVIKSVCKEKKAK
                     YIEVDFDDINIKKSDINSQIYDCTVMKETYRDLEIKLIGEHQINNSILAISVIKYLKD
                     INKLANINEESIRKGLINTKWPGRIEKIKENPIFIIDGAHNEDGAKSLAKALDKNFKG
                     RKLTLLIGMLEDKDIDSVLEILLPHFNKVITTTPSNPRAINSDILREKVLKYVDDVTS
                     KHEIEDAVNYTLETSSEDDIIISAGSLYMIGTVRTLVKKL"
     gene            complement(2923..3168)
                     /locus_tag="KR250_000334"
     CDS             complement(2923..3168)
                     /locus_tag="KR250_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03905 family TSCPD domain-containing
                     protein"
                     /protein_id="HBH0066305.1"
                     /translation="MKVTFNPSGVCCREMIFEVDENNVIVDAEFIGGCNGNLSGLRSL
                     IIGQNALEVADKLNGIDCGGKGTSCPDQLSKAIRQSI"
     gene            3316..4182
                     /locus_tag="KR250_000335"
     CDS             3316..4182
                     /locus_tag="KR250_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HBH0066306.1"
                     /translation="MKIYISNYLSKSISIVDYSTLELEKEIVLEDNIYPHHFCIEKEK
                     NLIYIPSSSNGILYVLDLSNDKIIDTVSIGGSLSQVVLSDNELFVANEDSNSIYVLDK
                     NTLNPIGIIGVDNMPHGFDFDRESKKLYVPCINSIVCIDTINKNIQKKINMDFRAWHI
                     ELDKQKKEMYISTLDGKLIIIDEVSMDIKKVLYEFLLPVEIRFNYSGKKVYVADLGYN
                     NVRILDYTTSRYIGNIEINGIPQGLEISKDEKLLFVSDTQENSVKVYETANNKLIKEI
                     KVGKEPTTIICL"
     gene            complement(4186..4722)
                     /locus_tag="KR250_000336"
     CDS             complement(4186..4722)
                     /locus_tag="KR250_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4364 family protein"
                     /protein_id="HBH0066307.1"
                     /translation="MFENSSEELAYHKLLILYILEKIKMDLTNSQITQVVLETEMMNY
                     FSLQQLLSQLMESKFLTTYKDSDREYYALTQRGVESLEYFFNRIPTSVTEKIDKYIDS
                     NKENLLADTQVKSSFVKQSDNEFIVNLRVIENQSNLIDLNLNVSSEKQAKLICNNWKN
                     NASYMYAEVIDLLIRDIH"
     gene            complement(4913..5116)
                     /locus_tag="KR250_000337"
     CDS             complement(4913..5116)
                     /locus_tag="KR250_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-type small acid-soluble spore
                     protein"
                     /protein_id="HBH0066308.1"
                     /translation="MSNSNRTVVPEAKAALNQMKLEIANEIGLSNYENIDKGNLTARE
                     NGYVGGYMTKKLVEMAERQMAGK"
     gene            5407..6171
                     /locus_tag="KR250_000338"
     CDS             5407..6171
                     /locus_tag="KR250_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBH0066309.1"
                     /translation="MKREVKRTKALIYSVLFFSLLLVLIFVFVYGIKYICSKSDNSKM
                     PIYKVDTNEKKIALSFDVAWGTDNMDDILKILDKHNVKATFFLVGSWVDDNEEIVKAI
                     DEKGHEIGNHSNTHANLKEISKEDITKEIETTSEKIYNITGKRTNLFRPPFGDVNNKA
                     MDICSDLGYKVIKWDVDSIDWKELGPNHVIEKVIKESQPGSIVLFHANINDVDNYLDT
                     IISRLKKDGYSLVKISDLLYKDNYVVDSNGVQKTKN"
     gene            6560..7873
                     /gene="prdC"
                     /locus_tag="KR250_000339"
     CDS             6560..7873
                     /gene="prdC"
                     /locus_tag="KR250_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase-associated electron transfer
                     protein PrdC"
                     /protein_id="HBH0066310.1"
                     /translation="MNKLYSILLKQHVGGPDKPVVSVGDVVKKGTLIAEPTGLGANIY
                     ASVSGKISEINDQAIVIEADEVQEDTFEPLKGEGILDLIKEAGVVGMGGAGFPTHVKL
                     NIDLNGGTILANAAECEPLLAHNIKEIEERPEIVYQGIKYAMEVTNAGKGMLAIKSKH
                     PKAIEAFKKVIKPGDNIEVAELVDMYPMGEERAIVRDVLGKLLEPTQLPSEANAVVIN
                     VETLTRIVEAVEQKRPVISKNITVVGQLNSGKESIVFEDVPIGTTVGELIERAGGIKG
                     EYGEIILGGPFTGKATTLDAPITKTSGGIIVTMPFVNEKRKMGLLVCACGPNEERMRD
                     IATKMGVTDIVSVQKCKQAQEIKGALKCENPGHCPGQAQKCIEFKKAGAEVILIGNCT
                     DCSNTVMGSAPKLKLGVYHITDHVMRTVNHPLIRRIKGDVRLKIK"
     regulatory      8277..8362
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            8944..11193
                     /locus_tag="KR250_000340"
     CDS             8944..11193
                     /locus_tag="KR250_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-Gln thioester bond-forming surface protein"
                     /protein_id="HBH0066311.1"
                     /translation="MKTKIKKSSIISLVIAFSMVFTAFTPIVSYADEVTSNDTILNGE
                     EQSNGQTSDVEKPSDGQVPDGEKPSDSQVPDVEKPSDGQVPDGEKPSDSQVPDGEKPS
                     DGQVPDGEKPSDGQIPDGEKPSDGQIPDGEKPSDGQIPDGEKPSDGQMPDGEKTSDSQ
                     TPDVEKPSDGQMPDGEKPLDEQTPEEEKPLEEEIVIEEMSLKQDIDKILDMTLSQINK
                     IVYNFWEDEEDVKADEQSEINQVFTSEDSFISLWYDKKAKVKNSCLLKEIDGSDRPYH
                     DLRFDDITKTLTFDYVIKGLVGASSKDDKYVIEGEDGEQTAFCYNNHLRPPSSNGKSP
                     YLPAEDFNGQENQNEEAVKSILYAGSEFDGFGYKQQFNLGGEENELMTYSATQSAIWI
                     ILGQMDEQERLKQYQGSINLCDKLIERAKTEEERAEYQKKKEVAINIKAYLEALLKAG
                     REELKPNNTGKPSLSNGLTKINFEKNEDGTYETEAVALVGYSGVVKLKLPDGVTAYDG
                     DGNIIGTGEVEISTQQKFKLKSVGKPDAKANISAVSYDYIFPKAIQYYKAVLDLGQKD
                     HSSSLPSSKQNLLSYTIEKKNGQEVNFNIGLPTDDDDVVNPPVPPIDDDVVNPPVPPT
                     DDTNGHKPKPSPPIDDTVINPPVPPMDDTIINPPVPPTEDAVLNPPVPPMEDAVLNPP
                     VPPTEDTVLNPPVPSVSDTVEKTPELSRDDTIIKSPKTGDETQLMSYVFISVIAICGL
                     AYQCKIKRN"
     gene            11600..13360
                     /gene="prdR"
                     /locus_tag="KR250_000341"
     CDS             11600..13360
                     /gene="prdR"
                     /locus_tag="KR250_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator
                     PrdR"
                     /protein_id="HBH0066312.1"
                     /translation="MFSIPEVKKVEEVMDTKFTTIDEDTRIEDAIKEMIKSNTKTLMV
                     IDSSDQLKGIISMTDIHNLYEMHKKYEGQPVKLIMKKDVIYVNEGLTLDECRDIMILK
                     NIGILPVLRDNKIIGVLKQEHIRDYLYMHLEDYGLTLKYIIGQIKEGICAINNEGVVI
                     LWNKFMEERYDIKSEDIVGRPMNEFLENTISEKVLNSKVGMSDLYFTDKKENMYALVH
                     ANPIFYKEEFIGVVCTEVDVTEAKILALELEKVNDTLKYLKNEVKNLSKGSFDKILGK
                     SYKLEKSKAIAKQVARTNSSIFIWGESGTGKEVFARAIHDYSERKGQFIPVNCSAIPN
                     ELFESEFFGYESGAFTGASKKGRIGIFELAKDGTVFLDEIADLPLSMQAKLLRVLQEK
                     EIRRVGGDTTIKINPRIISATNKDLSKMVKAEKFREDLYYRLNVVEIKIPPLRERKED
                     IGLLVHSFLEEICKQNNKPVLTISKDVIDIFQNYRWKGNIRELKNTIENIVVLSQNSK
                     IDVDDVPSYMMDSTNNSTEEEEYPLDLTKATQKIEIKNITKALKMSNGNKAKAAKILN
                     IPRTTLYYKIDQYKIDVSKI"
     gene            13789..15669
                     /gene="prdA"
                     /locus_tag="KR250_000342"
     CDS             13789..15669
                     /gene="prdA"
                     /locus_tag="KR250_000342"
                     /EC_number="1.21.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-proline reductase (dithiol) proprotein PrdA"
                     /protein_id="HBH0066313.1"
                     /translation="MSITLETAQAHANDPAVCCCRFEAGTIIAPENLEDPAIFADLED
                     SGLLTIPENGLTIGQVLGAKLKETLDALSPMTTDNVEGYKAGEAKEEVVEETVEEAAP
                     VSEAAVVPVSTGVAGETVKIHIGEGKNISLEIPLSVAGQAGVAAPVANVAAPVASAAA
                     EVAPKVEEKKLLRSLTKKHFKIDKVEFADETKIEGTTLYIRNAEEICKEANETQELVV
                     DMKLEIITPDKYETYSEAVLDIQPIATKEEGELGSGITRVIDGAVMVLTGTDEDGVQI
                     GEFGSSEGELNTTIMWGRPGAADKGEIFIKGQVTIKAGTNMERPGPLAAHRAFDYVTQ
                     EIREALKKVDNSLVVDEEVIEQYRREGKKKVVVIKEIMGQGAMHDNLILPVEPVGTLG
                     AQPNVDLGNMPVVLSPLEVLDGGIHALTCIGPASKEMSRHYWREPLVIRAMEDEEIDL
                     VGVVFVGSPQVNAEKFYVSKRLGMLVEAMEVDGAVVTTEGFGNNHIDFASHIEQIGMR
                     GIPVVGVSFSAVQGALVVGNKYMTHMVDNNKSKQGIENEILSNNTLAPEDAVRIMAML
                     KNAIEGVEVKAPERKWNPNVKLNNIEAIEKVTGEKIVLEENEQSLPMSKKRREIYEKD
                     EN"
     gene            15750..16034
                     /locus_tag="KR250_000343"
     CDS             15750..16034
                     /locus_tag="KR250_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0066314.1"
                     /translation="MDRLKYISSETFYEGIIVDIKGGGVTIDLKGRLGQFKIPNRMLI
                     TDYELKIGQEVGFMLSYPEVLSPEPNQEYVENIRREKEKQAEMQSKQEEK"
     gene            16072..16797
                     /gene="prdB"
                     /locus_tag="KR250_000344"
     CDS             16072..16797
                     /gene="prdB"
                     /locus_tag="KR250_000344"
                     /EC_number="1.21.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:16522..16524,aa:Sec)
                     /transl_table=11
                     /product="D-proline reductase (dithiol) protein PrdB"
                     /protein_id="HBH0066315.1"
                     /translation="MSLTTVQGLQSEIFVPITPPPVWTPVTKELKDMTIALATAAGVH
                     LKSDKRFNLAGDTTFRAIPNTATVDEMMVSHGGYDNGDVNKDINCMFPIDRLHELAAE
                     GFIKGVAPMHYAFMGGGGNQHVFTEETGPAIAAKLKEEGVDGVVMTAGUGTCHRTAVI
                     VQRAIEEAGIPTIIIAALPPVVRQNGTPRAVAPLVPMGANAGEPHNIEMQTHILRDTL
                     EQLVAIPSAGKIVPLPYEYKAHV"
     gene            16877..17635
                     /gene="prdD"
                     /locus_tag="KR250_000345"
     CDS             16877..17635
                     /gene="prdD"
                     /locus_tag="KR250_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase cluster protein PrdD"
                     /protein_id="HBH0066316.1"
                     /translation="MEEKIMRRLVIKPFHMNEVNFGSKTSIKKDVLTIDLSSIDEIKE
                     REELITDIKVDIIKPGDYDREINTIMDIIPISTKVLGRLGEGITHTLTGVYVMLTGAD
                     EDGNQMHEFGSSEGILKDQMVFGRYGTPSVEDYIIHVDVTLKGGLPFERTLPLAAFRA
                     CDDFIQEIRASLKMEDGRNATQVREYFDKIRPNAKKVVIVKQIAGQGAMYDNQLFSKE
                     PSGFEGGTSIIDMGNVPMIISPNEYRDGALRAMT"
     gene            17650..18117
                     /locus_tag="KR250_000346"
     CDS             17650..18117
                     /locus_tag="KR250_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase"
                     /protein_id="HBH0066317.1"
                     /translation="MGIGPSTKETSLHHFRDPLLDIVESDKDVDLLGVIVVGTPDGNE
                     NKTFVGQRTAAWLEAMRVDGAIVSSDGWGNSHVDYANTFEEIGKRDIPVVGVTFNGTQ
                     AKFVVSNQYMDTIVDMNKSKEGIETEVVGENNTNEIDAKKALAFLKLKMRKHG"
     gene            18135..18617
                     /locus_tag="KR250_000347"
                     /pseudo
     CDS             18135..18617
                     /locus_tag="KR250_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428088.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase"
     gene            18637..19644
                     /locus_tag="KR250_000348"
     CDS             18637..19644
                     /locus_tag="KR250_000348"
                     /EC_number="5.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline racemase"
                     /protein_id="HBH0066318.1"
                     /translation="MKFSRSIQAIDSHTAGEATRIVVGGIPNIKGNSMPEKKEYLEEN
                     LDYLRTAIMLEPRGHNDMFGSVMTQPCCPDADFGIIFMDGGGYLNMCGHGTIGAMTAA
                     IETGVVPAVEPVTHVVMEAPAGIIRGDVTVVDGKAKEVSFLNVPAFLYKEGVEVDLPG
                     VGTVKFDISFGGSFFAIIHASQLGLKIEPQNAGKLTELAMKLRDIINEKIEIQHPTLA
                     HIKTVDLVEIYDEPTHPEATYKNVVIFGQGQVDRSPCGTGTSAKLATLHAKGELKVGE
                     KFVYESILGTLFKGEIVEETKVADFNAVVPKITGSAYITGFNHFVIDEEDPLKHGFIL
                     K"
     gene            19764..20645
                     /locus_tag="KR250_000349"
     CDS             19764..20645
                     /locus_tag="KR250_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HBH0066319.1"
                     /translation="MVAILKALYVVFAAIFAVVFGKDVAKANKEGKLEEQSTPKVAVT
                     GFITDFFDTLGIGSFAPTIAMSKALKLNIPDKKMPGTLNVAHTIPVVTEAFIFTSIIP
                     VDGVTLVSMVVAAAVGSYIGAGIIAKMDERKIQLVMGVTLALTAILMLLGHPWINVLP
                     GGGNELGLTGVKLVIGVIGNFILGALMTAGIGLYAPGMAMVYFLGMSAKVAFPIVMGS
                     CALLMPVASMKFIKEDAYTKKASVIIAICGLIGVFVAAYIVKTLPMDILKALVIVVIA
                     YTSATMIMAANKNKKTA"
     gene            21313..21948
                     /locus_tag="KR250_000350"
     CDS             21313..21948
                     /locus_tag="KR250_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBH0066320.1"
                     /translation="MIAVKDENNVVNLRGELDNKLEFSHEIFGEKFYNMKIKINRLSD
                     SFDILPMTVSERLLQDIDLDKQNLVNVVGQLRSYNKTLNNKNRLVLTVFVREIKSIDE
                     ENKDPNSIFLDGYVCKEPVYRKTPLGREITDLLVAINRPYNKSDYIPSIVWGRNAKFA
                     KNLKVGDRIQLWGRVQSREYEKKIDENNVVKKMAYEVSISKIKKLDENNNE"
     gene            22132..22701
                     /locus_tag="KR250_000351"
     CDS             22132..22701
                     /locus_tag="KR250_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBH0066321.1"
                     /translation="MKSILCQINKQPVKAKYTSDELECFLDTIEYNVGDKILLVGHIG
                     NLGKRLRGLGTSVTILENSNYEDVCYSLVYNENCNVVKGKLEFLPFDDNQFNKIIIVD
                     QFNVITDCEKASSEIQRVLKGNGEVILEDLNLKNLKVKLNYLKHKFCGSVAKIHYPHE
                     VFNIFSRLNFDGMIKESNKLKYIYVGKRR"
     gene            22779..22916
                     /locus_tag="KR250_000352"
     CDS             22779..22916
                     /locus_tag="KR250_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0066322.1"
                     /translation="MEINYELIIIVMLFVILMSIQFTLNKIYLILKEIRQILMLKKNK
                     E"
     gene            22913..24688
                     /locus_tag="KR250_000353"
     CDS             22913..24688
                     /locus_tag="KR250_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBH0066323.1"
                     /translation="MMIMIGLEYIKSDIQNIAEAIVSVLNIDVTIVNKELFRIAGTGV
                     YIDKIGEKVDKYTAFKKSLTEQITILIDDPKSSDICRECYKSGACVEFAEVCCPIICD
                     GVSHGVIGLIAFTKEQAQIIENNKSGLINFLGKMADLISNKLKAQIKTYELELEKKKL
                     ETLLNNMDKAVVSIDVDGKIDKYNSKFKELFNIKDSITGKNIFVELDFIKKPSINNFK
                     KDKSCSFFYKRYGYDSKGIYNINKIVLKNELKGYVIDFIDKRDAIKNYNKMNKDYRIK
                     LENIIGESVAIRNSKKEALIASKSTSTVLITGESGTGKELFARAIHNHSNRVDNPFIA
                     VNCAAIPDNLLESELFGYEEGAFTGAKKGGKLGMFEVAHKGSIFLDEIGDMSLHLQSK
                     LLRVLQEKELNKIGSKSNVSIDVRIIAATNKDLEKMVQNGTFREDLYYRLNVIPIKLP
                     SLRERKEDIPLIIKHMVKEYSKKLDKNVIDIDSRVVDAMMEYKWPGNIRELQNIIEYS
                     VNMSSSSIITMNVIPQKIKNTSVEELEVKSDRIIPLDELEKIEIIKALNKYKDYKKDK
                     ELTAKALGISRATLYRKIEKYNIIS"
     gene            25113..26420
                     /locus_tag="KR250_000354"
     CDS             25113..26420
                     /locus_tag="KR250_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HBH0066324.1"
                     /translation="MRDIRKELVEMLKAEVKPAVGCTEPVALALACAKAKELLGEEIV
                     ENRMLVSPSIYKNGMCVGIPGTERLGLKIAAALGIVGGHSENGLSVLETLTKEEVKIA
                     EDYMDNTPLSITPADTREKVFIEVVLKGKNHIAKVRIRTKHDNFTFLEKDGEVLLDNE
                     PKVSASNDAAEKAESLMDTVTIQELIKNVEGIDFKDIEFLLDGVKMNEEMAEYGLKQK
                     TGIGVGYGIKKSIEEGLLGNDVINYAMMLTAGASDARMAGVKMPVMSSNGSGNHGLTA
                     ILPIVAYNKKFPQSDERLAKALAISHLVTGYIKNYTGRLSAVCGCGVAASTGATAGIS
                     WLMNGTEKQIEGAIENMIADLSGMICDGAKAGCALKLSSAASAAIQSAIIAKQDCFVP
                     PLNGIVGSSVEQSIQNLGRVSDKGMSITDEIILNVMNDMNKVD"
     gene            26748..27287
                     /gene="hpt"
                     /locus_tag="KR250_000355"
     CDS             26748..27287
                     /gene="hpt"
                     /locus_tag="KR250_000355"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HBH0066325.1"
                     /translation="MDIETKKWEVLYSEEKIKDKLRELGAIIEKDYKDKNLMVVSLLK
                     GSFIFCADLVRNINLPLRVNFMTTSSYGNNEESTGKVKVVSDVTTDIAGYDVLVVDDI
                     TDSALTMDFVLKHLKAKNPASLKCCVLLDKPSRRKVDLVPDYCGFEIEDKFVVGYGFD
                     FGDYYRNVPYIFNVTDEDR"
     gene            27665..29341
                     /gene="bclA2"
                     /locus_tag="KR250_000356"
     CDS             27665..29341
                     /gene="bclA2"
                     /locus_tag="KR250_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like exosporium glycoprotein BclA2"
                     /protein_id="HBH0066326.1"
                     /translation="MSDISGPSLYQDVGPTGPTGATGPTGPTGPRGVTGATGANGITG
                     PTGNTGATGANGITGPTGNMGATGPNGTTGSTGPTGNTGATGANGITGPTGNTGATGA
                     NGITGPTGNKGATGANGITGSTGPTGNTGATGANGITGPTGNTGATGATGPTGLTGAT
                     GATGANGITGPTGNTGATGANGVTGATGPTGNTGATGPTGSIGATGATGTTGATGPIG
                     ATGATGADGEVGPTGAVGATGPDGLVGPTGPTGPTGATGANGLVGPTGPTGATGANGL
                     VGPTGATGATGVAGAIGPTGAVGATGPTGADGAVGPTGATGATGANGATGPTGAVGAT
                     GANGVAGPIGPTGPTGENGVAGATGATGATGANGATGPTGAVGATGANGVAGAIGPTG
                     PTGANGATGATGATGATGANGATGPTGATGATGVLAANNAQFTVSSSSLVNNTLVTFN
                     SSFINGTNITFPTSSTINLAVGGIYNVSFGIRATLSLAGFMSITTNFNGVTQNNFIAK
                     AVNTLTSSDVSVSLSFLVDARAAAVTLSFTFGSGTTGTSAAGYVSVYRIQ"
     gene            complement(29924..30679)
                     /locus_tag="KR250_000357"
     CDS             complement(29924..30679)
                     /locus_tag="KR250_000357"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HBH0066327.1"
                     /translation="MLKVIISGYNGTMGRVLAECVKKDNELELVCGVARSADEKGHHS
                     DTKLYKTMTDVTEKTDVIIDFSHHDTLEPILSYALKNNTPVVLATTGYDENDLNKIEE
                     ASKNIPILLSSNTSLGVNILIKLVKDASKMLEGFDIEIIEKHHNRKEDAPSGTATMIA
                     NAIREVLPESENTHGRYGRDCKRKPNEIGIHSIRGGNIISDHDVVFAGDNEVLTLSHH
                     SQSDEVFANGSIVAAKFLIGKQSGLYKMSDILG"
     gene            31119..32108
                     /locus_tag="KR250_000358"
     CDS             31119..32108
                     /locus_tag="KR250_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HBH0066328.1"
                     /translation="MLKEEKTYQEKDAKVFNGVAALIMILLFLVVSIGIMVFGIVKLD
                     SGFTGIGSLMLILGLVFIVVDFILFFGLKMINPKEAIVLVLFGNYYGTIKKEGYYWVN
                     PFCSAINPAVSRINVSKSSSDEKVDISTNSRGKKVSLKTMTLNNEKQKVNDLLGNPII
                     IGVVVIWKVIDATKAVFNVDNYNTFLSIQCDSTIRNVSRLYPYDVSEDGDEKSLRGSS
                     QEIADRLKDELQSRVDIAGIEVCEVRITHLSYAPEIAAAMLQRQQAEAIIAARKKIVE
                     GAVSMVEMALTNLSENNVVTLDEERKAAMVSNLLVVLCGNKDAQPVVNSSSIY"
     gene            complement(32560..33276)
                     /gene="dapD"
                     /locus_tag="KR250_000359"
     CDS             complement(32560..33276)
                     /gene="dapD"
                     /locus_tag="KR250_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HBH0066329.1"
                     /translation="MVNLNDAYEIARYIKEVKKKTPVKVYVNTNSLHFQSTDKFKVFG
                     SNGSYIFVGDYEDIMEVLDKHGNDITDTHVEYDRRNSAIPLKNTLSEHARIEPGAIIR
                     DMVTIEKNAVVMMGAVINIGAVIGEGSMVDMNAVIGARGTLGKNVHLGAGAVVAGVLE
                     PPSATPVIVEDDVLIGANAVILEGVRIGKGAVVAAGAVVTTDVEAGAVVAGSPAKVIK
                     MKDEKTADKTKLMEDLRNLD"
     gene            complement(33500..34249)
                     /locus_tag="KR250_000360"
     CDS             complement(33500..34249)
                     /locus_tag="KR250_000360"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HBH0066330.1"
                     /translation="MLKVIATGYDGKMGKILADTIREDNELELVCVAARGLDSYEGDL
                     KIYEDMSTIVEEADVVIDFSHHSNLDNILSYVLKTKTPLVIATTGYNDDEMNKIREAA
                     KVIPVFQSYNMSLGVNVLVKLVKDAAKLLEGFDIEIIEKHHNRKVDAPSGTAVMIANA
                     IKEVLPNVENNYGRYGRSAKRQPNEVGIHAIRGGNIVGEHDALFAGDNEVLTISHQAQ
                     SRGIFASGSIAAAKYLVKQKPGFYNMDSMLG"
     gene            complement(34316..35203)
                     /locus_tag="KR250_000361"
     CDS             complement(34316..35203)
                     /locus_tag="KR250_000361"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HBH0066331.1"
                     /translation="MIFKGSAVALVTPFTKDNKVDFDKLGELVEYQIANGTDAIVSCG
                     TTGEANTMTDEEQLATIKYVVEKVNKRVPVIAGSGSNDTMHSVNLSQEAEKLGVDALL
                     IITPYYNKANKAGLKRHFETIANSVKLPIILYNVPGRTCVNISPSLIVELAKIDNIVA
                     VKEASGDLGQVAEIASLVPDDFAIYSGNDDTILPLLSLGGQGVISVLANVCPQETHDL
                     VAKFFEGDIEGSRKLQLGMDALIAALFIEVNPIPVKTAMNLLGFNVGDLRLPLAEMDP
                     ANLEVLKKELVNFGLKVNA"
     gene            complement(35320..36321)
                     /locus_tag="KR250_000362"
     CDS             complement(35320..36321)
                     /locus_tag="KR250_000362"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HBH0066332.1"
                     /translation="MKKVNVAIIGATGMVGRTFLKVLEERNFPIENLFLFSSSKSAGS
                     KVMFCGKEYIVEELKETSFTDRGIQIALFSAGGSISEKYAPIAASNGILVVDNSSQWR
                     MNPDVPLVVPEVNPEAIKNHKGIIANPNCSTIQAMVPLKALDDKYKIKRVVYSTYQAV
                     SGSGVKGVEDLEKGMNGNPTGFYPHQISYNCLPHIDSFTENGYTKEEMKMVNETMKIL
                     DNYDLKITATTVRVPVKNGHSESINVEFNNPFELDDLVKTLEEAPGVVVVDNPAKNEY
                     PTAVEFDDRDEVFVGRIRRDFSVDNGVNLWVVADNIRKGAATNAIQIAEMALSYDLV"
     gene            complement(36746..37627)
                     /locus_tag="KR250_000363"
     CDS             complement(36746..37627)
                     /locus_tag="KR250_000363"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HBH0066333.1"
                     /translation="MLFKGSAVALVTPFTEDNNVNFEKLGELIEYHIENGTDALVVCG
                     TTGEATTMSESEIFAVIKYTVEKVNKRIPVIAGTGSNNTMLSVHMSQEAEKLGVDGLL
                     IITPYYNKTNEKGLKLHFETIANSVKLPIILYNVPGRTKVNIKPSVVAELAKIDNIVA
                     VKEASGDLAQVAEIAKLVPKDFAIYSGNDDTILPLLSLGGSGIISVLANICPKETHDL
                     VTKFFEGDIEGSKKLQLDMDALIAALFIEVNPVPVKTAMNILGFNVGDLRLPLAEMEE
                     TNLNVLKQELTNFGFKF"
     gene            37950..39143
                     /locus_tag="KR250_000364"
     CDS             37950..39143
                     /locus_tag="KR250_000364"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HBH0066334.1"
                     /translation="MDTLKELFKIGSGPSSSHTMGPQRAAERFKNENPDAESFRAILY
                     GSLAATGKGHLTDYIIEKTIAPKKVEIVWEEDIIKDFHPNGMKFEALDKDKNVTAEWT
                     VYSVGGGTIAEEGQRNSKSNSIYHLDTMDEIVKWCKENNKTLVDFVLECEPKDIKDYI
                     KTIKDAMRKSIDDGLSTDEIIPGKLLLKRRASTFYNAYKKDKSFSTLVYAYALAASEQ
                     NASGNIIVTAPTCGSAGVIPGIFFAMQDFYNYDDEKIIEALLVAGIIGNIIKTNASIS
                     GAEVGCQGEVGAACSMAAAAVAYLKGGTIDHIEYAAEIALEHHLGMTCDPVYGYVQIP
                     CIERNAMAAQRAYDAANYALLTDGSHSVSLDQVVETMKETGIDMMDKYKETAKGGLAK
                     HFFSC"
     gene            39349..40467
                     /locus_tag="KR250_000365"
     CDS             39349..40467
                     /locus_tag="KR250_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyldiaminopimelate deacetylase"
                     /protein_id="HBH0066335.1"
                     /translation="MREASYLQEQLYKHRKSLNPIAEIGRKEYKTSKYIRDYLDKLGI
                     EYEVYLETGIVGVIEGKRPKKDIAFRADIDGLHSEEGVKHLCGHDGHATILLGLIEYL
                     NDNKEDLNDNIVFIFQPAEEGPGGAKALIDEGVLRKYNIDEIYGLHIYPELPEGYVGT
                     REGYFMAQIGDIDIEIVSKSGHGAMPQNGIDGIVIASNFVSSLQTIVSRNISPIDNAV
                     LTIGRIEGGARRNIIAENIKMEGTMRCFSPEVYEKMKLRVRELARGFELAYNCKVNIN
                     IIDDYIAVKNDKDLYQEFVEAIGSDTVVELEPLMISEDFSYYQKEVPGLFFMLGSRNE
                     EKGFVNGLHNINFNFDEKICVNALNVYSKLLKYKEAID"
     gene            40598..40843
                     /locus_tag="KR250_000366"
     CDS             40598..40843
                     /locus_tag="KR250_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small, acid-soluble spore protein, alpha/beta
                     type"
                     /protein_id="HBH0066336.1"
                     /translation="MKEKVQREKKITNGKNTKILTNDDIMKYEIASELGLMDKVGERG
                     WAGLTAKEAGRIGGMLTSRKKRLKKIVEEQNKKDESI"
     gene            40830..41576
                     /locus_tag="KR250_000367"
     CDS             40830..41576
                     /locus_tag="KR250_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBH0066337.1"
                     /translation="MSQYSDFAFVYDELMNEVDYNGWVKYIEDIIDSEGVKVKNILEL
                     ACGTGNLTIPLTKKNYDIAGIDISDEMLSVAREKSEKEGVELVLLQQDISELDFEISD
                     LDCVLCACDGFNYITYDDDLENVFSKTYELLKKDGIFIFDISSFYKLANILGNNMYGE
                     NREDIAYMWQNYFDEEENLIEMELTFFIKDECGKFERFEEIHQQRAYTEDEIVDMLEK
                     SGFKDIKYYGDFTFDKPSEESQRIFFVCKK"
     gene            41861..42736
                     /gene="hslO"
                     /locus_tag="KR250_000368"
     CDS             41861..42736
                     /gene="hslO"
                     /locus_tag="KR250_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HBH0066338.1"
                     /translation="MRDYVLRATSGNGQVRAFVATTRNTVEEARRLHETTKVATAALG
                     RTLTATSIMGLMMKNDSDKLTVIIKGGGPIGTIIATSDSKGMVKGYVGNPQVEVEDYP
                     NGKLNVAAAVGTEGVVKVIKDLGLREPYNGTYPLVSGEIAEDFTYYFAVSEQTPSVVA
                     LGVLTKEDEVEFAGGFIVQLMPDAEEETIAKLEENVAKLPSITNMLKEGKSPEDILNI
                     VLDGLEPKILDTCEVGFMCECSKERVKTALVAIGKKSLAQIIEEDKKAEVGCQFCNKK
                     YMYSEEELLEILKEM"
     gene            43127..43927
                     /locus_tag="KR250_000369"
     CDS             43127..43927
                     /locus_tag="KR250_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HBH0066339.1"
                     /translation="MESKTKYIGISILILLMIFLIAKNFNGKLEIPIPESNSRKFKSA
                     AVFYPQHQDDEVLWGGSAIVDAIKQCGVDNVYVVLVSDGSGVNVFKANTKFRNLTRKQ
                     KEELRNNEFKSALRELGVKPQNVIILADIDKKEGTHYELMEKTILDFEHKFKGNVTHI
                     AHHYKYDDHIMHRKNGEVLKKLKREGKIKDDLYFVKPKYVKYIPRNERAIYKAMDFDD
                     YNKVKSACYEYKVVNSKRKMYGIGYISAHSYFEHLLNDPELTSVLSED"
     gene            44454..46694
                     /locus_tag="KR250_000370"
     CDS             44454..46694
                     /locus_tag="KR250_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBH0066340.1"
                     /translation="MNNEFIILKGCKENNLNNISLKIPKRKITIFTGVSGSGKSSIVF
                     ETIAKESQRQLNERFSTFVRSFLPKYGEVKADCIENLSTPIIIDQSRLGGNSRSTLGT
                     ITDVNSFLRALYSRFGSEYIGNANMFSFNNIDGMCPHCHGLGKKLVPNMNQILDMNKS
                     LNEGAILLSGFGVGSWHWNIFAESGFFDINKRICDYSEVELEKFLHGKAEKIKIENAG
                     QTNMTYEGLMVKFNRLYLSREGEISEATKKKLSKLLIEDRCPICDGRRLNERVYRSLI
                     NGYNIADLTSMQIDELAEVIKSIDEPEAQPLIKGIIEKLNSIIDIGLGYLTLDRETSS
                     LSGGESQRIKMVKYLNSNLVDLMYIFDEPSVGLHPKDVYKLNNLLKKLRDKGNTIIVV
                     EHDPDVIKIADHIVDVGPKAGKYGGEVVYEGSYENLLTSGTLTGNALSKFLPIKESVR
                     EHSGYLEVKNCNKNNLKNVSIKIPKGVLTLVTGVAGAGKSTLIKDEFLKQNPSAVLID
                     QSPVSANSRSSLATYSGIMNNIRSIFSKTNGVNASLFSSNSEGACDNCKGSGIVEMNL
                     AFMESIKSTCNVCEGKKFKKEVLEYRFQGKNIIEVLEMSVLEAIEFFNLKQIKTKLQC
                     IEKMGIGYLTLGQTLDTLSGGECQRLKLASELNKESSVYILDEPTTGLHMADIENFIS
                     IIEEIVDNGNTVIIIEHNIDVIKRADWIIELGPEGGTKGGRVIFEGIPKQLCNNKISL
                     TAKYIV"
     gene            47108..48643
                     /locus_tag="KR250_000371"
     CDS             47108..48643
                     /locus_tag="KR250_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBH0066341.1"
                     /translation="MINDILTLLSERSDFFIKLSIEHLMISIISIIIAIVLGGIIGII
                     VSEFHNSSKPILGTINFLYTIPSISMLGFLIPFSGIGNTTAVIALTIYALLPMVRNTY
                     TGITNVDKNILEAARGMGSTDFQILYKIKLPLALPVIMSGIRNMVIMTIALAGIASFI
                     GAGGLGVAIYRGITTNNTIMTITGSILIALLALIFDFILGLVEKFFQRKRKKKNRKKV
                     FAILGSLILIATIFTSYLFNPSKTQTIHIATKPMTEQYILGEMLDILIEKNTGLNVEI
                     TQGVGGGTSNIQPAMENGEFDIYPEYTGTGWNMVLKKNELYTESMFSKLQSEYKTKLK
                     MQWTGMYGFSNSYALAVRKEVADKYNLKNITDLKDVSSKLTFGAEYDFYEREDGYTPL
                     CNTYGLNFKDTVDLDIGLKYQAINEGKIDVMPIFTTDGQYSVSDVVVLKDDKNFYPSY
                     QCGNVVRLEVLEENPELSKVFKMVGDILTDKEMAKMNYDVENNNLEPREVAREFLNKK
                     GLL"
     gene            48659..49420
                     /locus_tag="KR250_000372"
     CDS             48659..49420
                     /locus_tag="KR250_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0066342.1"
                     /translation="MTPIIQFKNIKKQYNDKTIIDNLNLDIEKGEFLTVIGSSGSGKT
                     TLLKMINGLILPDGGNILINQTDIKNEDLIKLRRRIGYCVQGSVLFPHMTVEENISYV
                     PNLLSKKNKLEVKSAVNKWMEIVGLPNDMKVRYPSELSGGQQQRVGIARALASSPEIL
                     LMDEPFGAVDEITRKQLQKEIKEIHKKTGVTIIFITHDIYEALILGTKTLVLNHGVVQ
                     QYDTPENILNTPANQFVDQLLNIRKSIIDDDNLKE"
     gene            49758..50627
                     /locus_tag="KR250_000373"
     CDS             49758..50627
                     /locus_tag="KR250_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBH0066343.1"
                     /translation="MDNNLITIEKTLDYIEKHLLDEMLDLNVISKEIGYSKYHLHRMF
                     TSVVGFSVHNYIQRRRLTEAARMLIFTEKPIIEIALISGYDTQRSFSRGFKKMFKRSP
                     NFFRRHQDFLPLQLKFDIHNRKKLRGDMVLDVKKVNCADINIVGYSGKVRKGFHIIGK
                     CWRDLHKNKEMIENKVDLGFLIGVSDYSNFKEIENTPTFTYIAGAQVATFGKLPNGME
                     KFVLPKSNYIVFTFIGKNEDSMQNVVEYIYQEWFPNSSCQFNENNLYDFVKYGEKTDE
                     NGLSEIQFWVPII"
     gene            50956..51207
                     /locus_tag="KR250_000374"
     CDS             50956..51207
                     /locus_tag="KR250_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3781 domain-containing protein"
                     /protein_id="HBH0066344.1"
                     /translation="MNVENELLKNLDRLHTTELGVVRIKKNLSLETNDVVNWCKTKIE
                     SPNAIINRKGKNWYISVYDCIITVNAHSYTIITAHKEKK"
     gene            51319..52098
                     /locus_tag="KR250_000375"
     CDS             51319..52098
                     /locus_tag="KR250_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBH0066345.1"
                     /translation="MNDTVKEKLAESLTSDTTDILPYLPYLLQDFWELGSSPEQMLQL
                     FRKHIPFNEDTKVLDLACGKGAVSVKLAKELNIHIKGIDLIPEFIDYARRKAIEYKVD
                     ALCDFEMNDANSSVKIEHDYDCVIFGAVGDILGNYKETISKLLPTIKSKGFILIDDAY
                     VVDKSTNEQLNFERVYPTYNEWCILFKELGLKLIECIPCLELENDVEEEMNWIITRAN
                     ELIKKYPSQKDMFEQYVISQKNEYTDLQQDLVGATWILQKE"
     gene            complement(52233..52883)
                     /locus_tag="KR250_000376"
     CDS             complement(52233..52883)
                     /locus_tag="KR250_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0066346.1"
                     /translation="MNIVMINGSPKVNKNNSQYFLSELKQLIQNDNKVFTFKVDNTSK
                     YPDALNAILNCDRLIIASPLYVDGIPSHLLHFLEKCEKFLKTQNKKPIPVYAIVNCGF
                     NEGEHNHLAIQMIEHWCKKINFTWVQGIGIGAGEMFGSLESVPLGKGPKKDLGIALKE
                     LSKNILSDNIHIDDSNKSTIFISPNFPRFLYKLMGNHQWNTQAKSNGLTKSDILKK"
     gene            complement(52880..53383)
                     /locus_tag="KR250_000377"
     CDS             complement(52880..53383)
                     /locus_tag="KR250_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBH0066347.1"
                     /translation="MRLLIHDLTDKELEKLLPKLDDNIKILPNTKEIHKCIGCFGCWI
                     KTPSECVVKDYYSNMGKLINDSDGIIIISRCCYGGFSPFVKNVLDKSINYILPYFVIK
                     DNMMRHKSRYDKQINLKAIFYGDCITEAEKETATKLVNGNAINFNIKEHSVCFYENIQ
                     DIRGESI"
     gene            complement(53432..53947)
                     /locus_tag="KR250_000378"
     CDS             complement(53432..53947)
                     /locus_tag="KR250_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBH0066348.1"
                     /translation="MLEYIILGFLMEKELSGYDLKQIMSESTSYFFDASFGSIYPALK
                     RLETKGYIHYHEVIDGNKLKKLYSITNTGKEVFLEWLKKPINFSKTKQDHLVNIFFYK
                     YLPKEIIETNLKMLITEVELLLNKLSKQKNDAEENHCVDQYTYMYATMIYGVDYYNFV
                     INWCKDLLKNL"
     gene            54227..54751
                     /locus_tag="KR250_000379"
     CDS             54227..54751
                     /locus_tag="KR250_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBH0066349.1"
                     /translation="MHEQIDCCERKKRTIDIKGENMRELLKWVSIINRYNTNYIDRKL
                     ADIGINNSQYFYVLHICNNPGITQDTIFEKILVNPSNITRALANLEKEGFITREPGVK
                     DKRTWHLYPTEKSLGCYDEILKITNGYVEELLSSFEKEEQELFMSMLKKVAFLAIDMN
                     EEAKLEAKENGRKI"
     gene            54735..56138
                     /locus_tag="KR250_000380"
     CDS             54735..56138
                     /locus_tag="KR250_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBH0066350.1"
                     /translation="MEEKYKEPLEYEKVSKLLTKYAIPSIIAMLVSALYNIVDQIFIG
                     QGVGVLGNAATNVTFPLTTICTAIALLLGIGGASKCSLELGAKNSEKATKAAGNSICL
                     MAVFGISLFIVVSIFLTPLLKFFGSTSEILPYAHTYTKITSIGLPFLIMSTGMSKLIL
                     ADGRPKASMFCMLIGAVINTILNPLFIFVFNMGIAGSALATVIGQLISFGISVHYIKN
                     FQHIKLHKKSFKLDSIIYKHIFALGSSSCFNQLAMTVTQIVMNNTLAYYGARSVYGSE
                     IPLACVGIIIKVNMIFISIIIGISQGMQPIIGYNYGAEKYNCVKEAYKFAVGSATIIS
                     VIAFLCFQLFPRQITSIFGNGNETYFIFAEHYFRTFLFLTFINGVQIVSSTFFTSIGK
                     SVLGLFTSLTRQILFLIPLIVILPMFMGIDGVMFAGPIADAAAAMVCIYFAVHEMKSL
                     TIKQNNLMSNHNKIAEV"
     gene            complement(56702..57013)
                     /locus_tag="KR250_000381"
     CDS             complement(56702..57013)
                     /locus_tag="KR250_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0066351.1"
                     /translation="MKKIIKSFTFWFFLIALFEIYMHQIGQDSKSIVLIGLNPILSII
                     SRVDSFFVFMDSGMQIPCRTITGSISIYWYIASILSFLIYGIILDLIRIVISKIGNKT
                     K"
     gene            complement(57069..57662)
                     /locus_tag="KR250_000382"
     CDS             complement(57069..57662)
                     /locus_tag="KR250_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBH0066352.1"
                     /translation="MISDSISKRRSIRKYKNQSISHETIEKIIEAGINAPSSKNRQPW
                     RFVVITEKEKESMLKAMSKGIQNEINDNGLLPGSRQHIAGANYTVEIMKQAPVTIFIL
                     NILGKSPLEKLSPEERFYEMANMQSIGAAIQNMSLTAVELGLGSLWICDVYFAYRELC
                     EWLNTDSQLVAAISLGYPDEEPSRRPRLQLSDVTEWR"
     gene            58513..59163
                     /locus_tag="KR250_000383"
     CDS             58513..59163
                     /locus_tag="KR250_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HBH0066353.1"
                     /translation="MDFISSILQGLLSILFNFTGDFGISIVLITLIVKLVLMPLSLKQ
                     RFSVKKQQELAEKMEYIKEKYKNDTKELEKQIQVSSVESMKSMLGCLIVLVQMPVVYA
                     LYHVCLDMTKEFTTIIIPWIANLGTFDNLFILPCIYTLIMLASNLVNYIPYLKINSQV
                     KFNKQMAVVTTITSLILTVRTPVAIGLYFITSAIYSLIEDICFRIYFNRKNRIVTK"
     gene            59366..60037
                     /locus_tag="KR250_000384"
     CDS             59366..60037
                     /locus_tag="KR250_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0066354.1"
                     /translation="MAKILVVEDEKRMQNIIVEYMQKGGYTCITADDGVEALTILKSN
                     NIDLMILDIMMPYLDGFSVCRVSREMTNIPIIILTAKGEEEDKLKGYEYGADDYITKP
                     FSPKVLLAKVNALLRRYTTDIPKNSLSLGKIFIMVASRQVYVEDKLIDLTYKEFELLR
                     LFMENPNQVFSREKLLNCIWGYDFEGNTRTVDTHIKTLRKKLGSEGHHIVTLIRSGYK
                     FEVKE"
     gene            60034..61164
                     /locus_tag="KR250_000385"
     CDS             60034..61164
                     /locus_tag="KR250_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBH0066355.1"
                     /translation="MNSLRKIIEKLKNVDISNWIKQNFGLYSVRKKVFLLSKLSGALI
                     ILFYLVVEEFSINSKFEFWIWFIILVIFIMSIDFLLGKFISEPITSINKSAKSMSQLD
                     FSNPCTVNTNDEFGELSRSLNTMSTNLQQALSDLESANIQLEKDVNKERMLLEQRKEL
                     VDTISHEMKTPLGIIRAYTEGLIDEVDEEKKKNYMNVIIEETDRMNNMIVSLLDLSAL
                     EAGVSKLNPERFDFIEFVETVAGRLLIDIPDANFYFTYDLPEYEVFVVADKMRMEQVV
                     ENLVINAKKHVVYNGNLDLSITCENGLLLFEIYNDGRFIEQDEISKIWSKFYRSVQSQ
                     RTGGSGLGLAIVSQILTMQGLEYGVNNRDRGVEFYFMIPIDE"
     gene            61595..62440
                     /locus_tag="KR250_000386"
     CDS             61595..62440
                     /locus_tag="KR250_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0066356.1"
                     /translation="MIKVRELKFSYGKDKQILHGLNFEVKEGEIFGFLGPNGSGKSTT
                     QKILNGVLKGYNGHVSIFGKEVKAYTESLYQKIGVLFEFPYLYTNLSAIDNLEYFSSF
                     YPKNQRRDIYELLDLLEFKKEFINKPVSSYSKGMKQRISMARALVSNPKLLFLDEPTS
                     GLDPSGAVLFRKIIEEERKKGTTIFLTTHNMLDADLMCNNVAFIADGKIMVIDKPKNL
                     KMKNSNNKVEVEFVYNGNRDIEIVDIQELESGITFKYDEILSVHSKEPTLEEVFIKYT
                     GRMLV"
     gene            62440..63468
                     /locus_tag="KR250_000387"
     CDS             62440..63468
                     /locus_tag="KR250_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0066357.1"
                     /translation="MYSKFFTLVKKDFWMMIYGKFFVLTLGSLILYSCYINFGYVKFM
                     DEDSYHNNVYLYNPENISMSASPLINQVSSIGELKAKLDEDTSAVCIDMSSSKPKVIL
                     HESIKGVDNYRADYALSLLSSEKDHNSKISDNNAIIQINDKDTSEIKQRKDMTCEILF
                     FEIVAIGFLGIASLLFKEKQMGVMRITGILPVKRSLFILSKIFAFFICDLCFTVLLVV
                     FNIGFLEGIKILPQVLVQASILSIIMSLIGIGCSMLLRDFKQFSLIYLVVAVFITTPI
                     FLSANTSFEIDWIKYHPFYHVYSQLKNAFFMNFSKELVYFVVCSLTIIVLFLLVKYIF
                     NKELVREG"
     gene            63471..64484
                     /locus_tag="KR250_000388"
     CDS             63471..64484
                     /locus_tag="KR250_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0066358.1"
                     /translation="MIGLRYQLKSIKKDKMCIISFFLPVVMAILINFMGTIDISSLGE
                     IRFGIVKDTLPVQTEQWLKKYGMVSVYDTKMHLIREIKDIKTDTIGVVRDGKGIKTVF
                     YGNEINAIKNVGKTLPEMFRLKGDLKDVEVTILPKDSILKEFQNVFIAMILITAMFMG
                     CTFNAMNIISEKEDGISFINEVLPQSRSQYIIQKVFLGLLCGCLSSMITVFICLDISI
                     LDMIMMLLLVVFSAFISSLIGLFIGKLSEGLMVGIVYIKIVMILFIGTPLLIYLLGVN
                     TSGIGNLCYIMPSVATFNGIMEIINGNVIIIKDIVILAVHCVVWFMIYIILDKKRKLS
                     FSR"
     gene            complement(65001..66251)
                     /gene="cme"
                     /locus_tag="KR250_000389"
     CDS             complement(65001..66251)
                     /gene="cme"
                     /locus_tag="KR250_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Cme"
                     /protein_id="HBH0066359.1"
                     /translation="MENKNTNSTWKKKVILFLASQYISLFGSSLVQMAMIWYVTLQTS
                     SGVWVTVLTVCSFIPQMIISFFAGVWADYYNRKVLIIVSDSTIAISTLVLILFMMSSN
                     GTTSELIAISVISVIRSLGSGVQTPAVNAMIPQLVPEEHLMRFNGINSSLQSIVQFIV
                     PIVAAAILGFGNKINQVLLIDVFTAIIAIVLLIFIKIPKHDIPKQTEKVSFFADMVSG
                     IKYCFSDKFIRKVLFTYGAFMLLCVPSGFLIGLLIERTFGKGYIYLSIIEMIGFLGMV
                     LGGLIIGVFGGFKNRNKTFFLAILSYAIFSIILGLVTQAWQFFIFMFFTNFSIPIIQA
                     SAMTMLQEHVAPQMLGRVLSLPIIIYTGFIPLGMMIFGPMADVISINNLIIIAGIILL
                     FFALAIPYSKQFYKQGISKSNESE"
     gene            complement(66337..67083)
                     /locus_tag="KR250_000390"
     CDS             complement(66337..67083)
                     /locus_tag="KR250_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBH0066360.1"
                     /translation="MDTYTTSEVADLIGVHPNTVRLYEELNLIPKPKRNTNGYRIFND
                     FHIEQFRFARTALKIEVVQRGLRKDALEIVRTSANRDFDKAISLVNQYLQQLEIEKSN
                     AEEALTIVAQILNGNTPETNKLFLTRKETAEHLNVTIDTLRNWEMNGLLKVKRKQNGY
                     RVYTDEDIKRLKIISALRCANYSLSAILRMLNALSSSPNTNIKTVLDTPKDDDEIISV
                     CDKLITSLKEAEHNAKTMKSHLQIMKKQFS"
     gene            complement(67236..68258)
                     /locus_tag="KR250_000391"
     CDS             complement(67236..68258)
                     /locus_tag="KR250_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HBH0066361.1"
                     /translation="MNKNNCLDLIFEKVDPSSVGMSSEKLSQLEPLIISQYSNINGII
                     IARKGKIVFEKYYNGYNADDTFHVASVTKSIISALIGIAIDKGYIKDVDQKVVDFFPE
                     YTCSPSEICKKAVTIRHLLTMTAPYPFKNMTESLERLCKQKDWIKYTLNQLGQKGEIG
                     TFKYSSAGTHLLSAIITKTTGKSAREFANEYLFKPIGMKVIPDYDMKDFGFEDFFGKN
                     LKGWAKDRQGYSIGGWGLTLSARDMLRFGFLYLNDGIWNNKRIISKSWIEESVKMNSN
                     EYGYLWWLCKKDGVLWYSAMGTGGNAICCIPQYDLVIAIPSKIVFKPRDRWEFIEKYI
                     LTSIID"
     gene            complement(68258..70678)
                     /locus_tag="KR250_000392"
     CDS             complement(68258..70678)
                     /locus_tag="KR250_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBH0066362.1"
                     /translation="MKEIKINGAKIHNLKNIDVSIPKDKLVVATGVSGSGKSSLMFDI
                     VFEEGRKQYLQSLGILAGIDSEDKFDNIQGLAPTIAVQQNIIRQSNPRSTVGTRTNIL
                     NMLTLLYSVEGQIMCTMCDTPVSDNLICNNCGYEEERLRPSYFSYNSSDGMCMKCSGH
                     GAYFEINIEKLVLDKHDTLKQVLDRAKITPGYLRVFSKKFNDYLDIQFLQLPEEIRNE
                     VLYGHYENGKKSSSLSKVFHNRYKRGEDLNGVYTMTACSDCNGFKIGEEARRVLLNGK
                     HIGELGKMTILEIDDFLKLLINQGNLNTFGTNLVNDILSKTQHLIESRLGHLSLYREM
                     STLSGGEMQRLFLNSHLDSEMDSLIYVLDEPTAGLHESEKIDILKSLKKLKDLGNTVI
                     VVEHDKNTIEMAEHIIDIGPKAGVEGGQLVYQGDLEGLLQSDKSLTGQYLSGKYPMPN
                     RTSNKNITHTDKIPCITIQNANTNNLKNVTVSLPLGAMVGISGKSGSGKSSLISDTLL
                     PLLRSQFNNHTINNQINSSESEIGEEDDYAVVETIADRLIGTKHISGYSEISQSPIGK
                     NMNSTPASYIGIWDKIRTLFAGQPESLEQGFTAGHFSFNSKGACPKCSGSGYEKVWLG
                     NNLSIDHICSECQGKRFNDESLSIKYKNKNIHDILNMSVSESVHFFKDIPNIVSHLNV
                     LEQIGMGYIKLGQPTPTLSGGESQRIKLAKEIGKKRNGNILYVLDEPTTGLSLYDTSK
                     LIQLLDELVENGNSVIVVEHDIDVLNVCDWIVELGPEGGDKGGYIIAEGSPKTLKENP
                     KSITGRYL"
     gene            complement(70764..71684)
                     /locus_tag="KR250_000393"
     CDS             complement(70764..71684)
                     /locus_tag="KR250_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBH0066363.1"
                     /translation="MTNAKSKKEYLNRICKVQDYIEEHLHEPLSLDELSNIAGFSKFH
                     FHRIFKAIEKETLAQYVNRLKLENATAFLIHRTDMTVTDIAHYFGFTDSAVFSRAFKN
                     YYKISPIKYRNNYSKNCKEPYKISQYNRSISKPKCKNTREVKGEIEILELDNMNTIYT
                     RYTGSYGNLTSTFPKLLERLFTYASEQNLLGLENTQILAIYHDNPQFTQEHQLRTSIC
                     MTIPDNIVVKENDNLGNMIIPSGKYLVGHFDISKSEYSDAWDFMYNEWLSNGNYKLRA
                     SFPFEVYLNNPHNHPQNRHLVDIYLPIEPF"
     gene            72274..73200
                     /locus_tag="KR250_000394"
     CDS             72274..73200
                     /locus_tag="KR250_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conserved phage C-terminal domain-containing
                     protein"
                     /protein_id="HBH0066364.1"
                     /translation="MSNSFYQLNSAVLYFNEKRDVNRIRFNHYVMRKENIQRLKGTLP
                     RGQFMMTVRKAASDLDVSISTISRLVNEFIDLGILRLISRGVKGNCCSVYSYVCSESS
                     NAEIVTKNGDIFKNIEKYIDATINNNQKTMKYKHRNELFKNSNTNIYSRINQESIDKD
                     YDLIIHTGYETENETKKKELLKNNLNKKNLNKNTFENSDEDLTEDSTYKNPESIHEII
                     IRKLNSESGKNFKADLSITKALIDKRLKEGYSLEDFYKVIEAKVRRWRYTDMEIYIEP
                     TILFGYEFESYLNEESSLSACNYGDMCYEDMY"
     gene            complement(73583..75235)
                     /locus_tag="KR250_000395"
     CDS             complement(73583..75235)
                     /locus_tag="KR250_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp21"
                     /protein_id="HBH0066365.1"
                     /translation="MNKKNLAILISGMMFFSSSSAIFADNTTKQERLIGKTRYETAVE
                     VSKLGWVQSKTAIIVNGNSIQSALCANPFAKLKNAPILLVNNNSIENSTKAELKRLGV
                     DNVYIVDSGNSISSKVENEIKSLNIKINKIVGNNIYEMSTNVLKEIDKIKKIENVAVV
                     NGLKGLSDAVSIASPAAIHNMPIILVSDENSNDSSINFLNNRSIKKSYIVGGNKILSD
                     NTVKKYPNRERISGSDRNNTNAKIIDKFYKDKKINNVFVAKDGINQENALVDSLSIGV
                     LAAKENSPIMISNGSLVNSQKEFIKNKDIDEITQVGGGYNQKAFEDILKLKNVNIRNK
                     TKKISLIQAKEILKQALPGSNILVCYYDEDDNEYDCKVIKDNILYEIEIDAYSGKIIE
                     IEKDDDLNDNNLGTQNISLDKAKSIALDAIPNGNLIYCKYDAEDNEYEAKVIKNKKTY
                     ELEISAFDGRIVDIDMDNDSDDDFDDNDKNNSNTNDNSSIISAEKAKSIMLDKVPGGK
                     IVKFKYDEDDKEYEGEIIKGKTEYEITINAITGKILEFEIDD"
     gene            75834..76955
                     /locus_tag="KR250_000396"
     CDS             75834..76955
                     /locus_tag="KR250_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBH0066366.1"
                     /translation="MEAKKLGFGLMRLPIKDENDVTTIDMEHLNKMVDTFLERGFTYF
                     DTAYVYHMGKSEIALRESLVKRHKRESFTIATKLPLMALKKKEEQETIFNEQLEKCGV
                     DYFDYYLLHNIGVSHYEVAKKFDSFKFIEEKKKEGKIKNIGFSFHDSAELLDKVLSEH
                     PEVDFVQLQINYLDWDNESIQSRKCYEVAEKHNKPVIVMEPVKGGTLAKIPEKAEKLL
                     NDYNPDMSISSWAIRFAASKDNVMMVLSGMSNMEQLLDNTGYMQNFKPFVQEEYNIID
                     EAVEIINESILIPCTACQYCVEGCPKNIAIPNYFALYNAEKQALNTGFSTQMVYYNNY
                     TKTYGKASDCIECKQCEESCPQHIKIIDALKNVAETFEK"
     gene            76991..77404
                     /locus_tag="KR250_000397"
     CDS             76991..77404
                     /locus_tag="KR250_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBH0066367.1"
                     /translation="MTISEVSKKYELSADTLRYYERIGLIPPVNRNKSGIRSFTEEDC
                     EWVNFIKCMRGAGLSIETLIEYVAMFQQGSSTIKARKELLIEQRNQLAKRIEEMQKTL
                     ERLNFKIDRYEEGIIEKEKVLKSSRNKKMEAVSYE"
     gene            77397..77936
                     /locus_tag="KR250_000398"
     CDS             77397..77936
                     /locus_tag="KR250_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBH0066368.1"
                     /translation="MSKNVLIVSASPRKGGNSDLLCDQFIQGVLESKNQVEKIFLKDK
                     DINYCTGCGACYEKEANCSQTDDMGEILEKMIGADVIVLATPVYFYTMNAQMKTMIDR
                     TCSRYTEICDKDFYFIVTAADENVADMERTIEGFRGFTSCLNGSKEKGIVYGVGAWNV
                     GDIKGSKAMKQAYEMGKLA"
     gene            complement(78136..78621)
                     /locus_tag="KR250_000399"
     CDS             complement(78136..78621)
                     /locus_tag="KR250_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0066369.1"
                     /translation="MSKSELTAETTEEMLEVLSGKDYDIACHLHELGKSLDCKIEPKT
                     GARSYKIVYSTKKPKRSLFTIECNEKKWRVKANLFHLNTYKDAVEECSKTIKDSITKT
                     RTCKKCNSKCIGGSSFELDGKSYLTCIGSGHYFANMEEMDWKNLEKLITKENNIMQES
                     V"
     gene            79526..79903
                     /locus_tag="KR250_000400"
     CDS             79526..79903
                     /locus_tag="KR250_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003483933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HBH0066370.1"
                     /translation="MSKVVIATEKAPGAIGPYSQAIQVGNIVSTSGQLPIDMKTGELV
                     SDPKEATKASLDNVKAILEQAGTSMDKIFKTTVFVKDLKDFNDINEVYATYFKENPPA
                     RSCVQVAQLPKDAVLEIEAMAVL"
     gene            80299..82104
                     /locus_tag="KR250_000401"
     CDS             80299..82104
                     /locus_tag="KR250_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBH0066371.1"
                     /translation="MLSKLKEFQQEMIKYTETVASVLDVDIEIVDDRLIRISGTGLYK
                     SKINESVVTEGFIYDNVIQTGQELVVLDICDNQLCIECSHYMKCLNKVIIAVPIKYNN
                     RTIGVIGAISTDKTKKVEISAKIDNYLKFVNHICDLISMKIEEHEASKNSSRKMDMMI
                     EIIENVEKGVIILDINSKISYINNIALKKLDIDKNIIENIVNIVSVESSSNGHELLEI
                     DIDNKIYNINAKIIPVYPYINQYDKIIIFDKTYINHKGHGKVNSGWGNSDIESIIGNS
                     EAMLKVKERTKKLAKSNSTVLITGESGTGKELIARAIHAEGSRWNKPFIAINCAAIPE
                     NLLESELFGYIKGAFSGASSGGKVGKFELANEGVIFLDEIGDLSMPLQAKLLRVLQER
                     KFARIGSNKLIDLDIRVIAATNKNLLKLVNEGKFRDDLYYRLNVIPINLPPLRERKDD
                     IEAIMMKFASKYSLELGIQLNKIEENVMNMLINYNWPGNIRELENAVEYMMNLVGDDG
                     IIYKDMLPLDILNYYNINGNIYKNKDVNIIFEDDIVGGIVENQERILSIKELELTYIN
                     KLLNKYGRDTKTKKKIAKDLGIGLATLYRKLEEEQ"
     gene            82181..82718
                     /locus_tag="KR250_000402"
                     /pseudo
     CDS             82181..82718
                     /locus_tag="KR250_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427977.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
     gene            83332..84546
                     /gene="dpaL"
                     /locus_tag="KR250_000403"
     CDS             83332..84546
                     /gene="dpaL"
                     /locus_tag="KR250_000403"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HBH0066372.1"
                     /translation="MREIKWKLNNLPKSEEKEKGIEFLKDEEIAKAKAFHESFPQYEK
                     TPLVKLDNLAKSLGVSGIYVKDESYRFGLNAFKVLGGSYSMGRYLAQRLNTDISELGY
                     DKLTSKEIKEKLGEITFFTATDGNHGRGVAWTANKLGQKSVVLMPKGSSEFRLNKIKG
                     EGADASITDLNYDDAVRLANDYAEADDHGVMVQDTAWDGYEEIPAWIMQGYGTMAKEA
                     IEQLKEYGVDRPTHVFVQAGVGSLAGAVQGYVASIYDECPITVVVEADEADCYYKSAE
                     AGDGKPRFVGGDMPTIMAGLACGEPNTIGFEVLKNYSSAFVSAPDWVSAKGMRILGNP
                     LRGDEKVISGESGAVTTGALVSILESEDLKDLREALKLDENSKVLLISTEGDTDPDKY
                     RDIVWNGECQSK"
     gene            84879..86066
                     /locus_tag="KR250_000404"
     CDS             84879..86066
                     /locus_tag="KR250_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HBH0066373.1"
                     /translation="MLNETRKKQLTEVCQDLIRNASYSGQEENVVKAIEENFALLGFD
                     SWSRDRYGNIVGCIKGNKPGKKILFDGHIDTVPVPDASKWSVPPFEARIVDGKIYGRG
                     TSDMKGQVSAMMAAVSYFAEDTNKDFEGELYVAGVVHEEIFEGVSAREISKAVQPDYV
                     VIGESSELNLKIGQRGRGEIVVETFGKPAHSANPEKGINAVYKMANVIQKIQQLVPPT
                     HPVLGDGILVLTDIKSSPYPGASVVPDYCKATFDRRLLVGETREGVLAPIQALLDEMM
                     KEDPELNAKVSYAVEKADCYTGNTIESERFFPGWLYDEEDEFVQAAYKGLKEAGIDSE
                     ITQYSFCTNGSHYAGEAGIRTIGFGPSKENLAHTIDEYIELDQLFKGAEGYYGILKSV
                     YKK"
     gene            86082..87665
                     /locus_tag="KR250_000405"
     CDS             86082..87665
                     /locus_tag="KR250_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HBH0066374.1"
                     /translation="MRTLIKHGLIVDGNKTPAYEGDILIENEKILKISQDLNEEADKV
                     IDAKGRVICPGFIDTHSHSDLVILVNPYNEVKIRQGITTEVLGQDGISMAPLPQEHIS
                     SWRKNLAGLDGESDEIDWKYETTENYLKMMDYNGVGLNETYLVPHGNVRMEAMGLEDR
                     PATKEEIQKMCEITERELKAGAIGLSTGLIYIPCAYSLTEEIIEMCKVVAKYDGVFVV
                     HQRSEADTILTSMEEIIEIGKQSGVKVHFSHFKVCGKANWKYIPQVIELLEKAEKEGI
                     RVSFDQYPYAAGSTMLGVVLPPWAHSGGTDKLIERLSDENERAKMKKDIANGIEGWDN
                     FIEFAGIDQIFVTSVKTEKNKDTIGKSLLEIGKMRGKDPLDATFDLLKEEENAVGMVD
                     FYGLEEHIIGFMKRDEQNVCTDGLLAGKPHPRAYGSFPKILGRYVRELNVLTIEEAVY
                     KMTKKAATSFSIKDRGELKEGYFADIVIFDKDTVSGCDDFINSMQYPTGIDYVIINGN
                     CVVEEGKYNKIKAGKVLKN"
     gene            87693..89021
                     /gene="ssnA"
                     /locus_tag="KR250_000406"
     CDS             87693..89021
                     /gene="ssnA"
                     /locus_tag="KR250_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HBH0066375.1"
                     /translation="MILVGNGRLISQDSLNPYMEDGCVVINDNLIEDIGTTEEMKAKY
                     PVHEFIDAGKKIIMPGLINSHMHIYSSFARGMAVPGKPSENFLEILNNLWWRLDKQLT
                     LEDTKYSAYATYIECIKNGVTTVYDHHASPNAIEGSLFTISDVAKDLGIRTCLCYEVS
                     DRDGGNTIQQGINENVNFIKYAQKDDTDMIKGMFGLHAAFTLSDDTLNKCASEMAGLD
                     TGYHVHVAEGLADLQYNVDKYGKRVLERFNDFGVLKEKTIAVHCIYISAEEISLLNKT
                     NTFVVHNPESNMGNAVGCSPAIELLKRGVTVGLGTDGYTSDMFESLKVANIIHKHHLK
                     DPRVGFEESQTMLFDNNRKIAKAYYKNDLGVLKKGAYADVIVVDYIPHTPLTENTIGG
                     HTIFGMSGRSVDTTIINGKVLMKDRKLINIDEESILAKSRELSQNLWNRI"
     gene            89166..90518
                     /locus_tag="KR250_000407"
     CDS             89166..90518
                     /locus_tag="KR250_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HBH0066376.1"
                     /translation="MSRDKSPKIAPVDERLPVSKSWVFSLQHVLAMCAGAVAVPMMIG
                     SAARLDHASIVFLINACLFMAGIGTLIQSVGIGNIVGAKIPVIEGTSFAAVAGMTAIA
                     SSYSDSQVAIQTVFGSVIVAGLFCILLAPVFEKLIRFFPSVVTGTVVMVIGISLFPVG
                     IKWITDNKLEAVEPNSLALALAVLAITLLSFKFLKGIWNSTAILVGIVLGTLLSMAVG
                     MADFSAVNGVGWLNLNVPLKFGMPRFEPSAILSLCLIMLVLMTESVGNMIAIHEMVDK
                     EVTGKNIKRGLFGDGISTALSGIFNSFPITPFGQNTGIVGLTGIKSRFIAVYAGVILL
                     VLGFLPKFAAIIGAIPKPVLGGVGFAMFGMVMVGGVKTLSKVKFDGTKNGVIVAVSVG
                     LSMIPLTNPDFYHNFPTWVQTIFHSGITTGSLSAVLLNIFFNELGSKKEKNNKVEEAK
                     "
     gene            90710..91819
                     /locus_tag="KR250_000408"
     CDS             90710..91819
                     /locus_tag="KR250_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBH0066377.1"
                     /translation="MANLKVNLYGMEFLNPIMTAAGPGAKDGALCQEAAKGGAGAICT
                     KTISVLPADVPRPCMAKTNSGFLNTELWSELPKEQWIEEEYKKVKETGLPVIVSMGYS
                     AEQIAEVAPLVKPYADAVELSTHYVGTDVAPIVNALKAAKAVLDVPVFMKMSPHTDIQ
                     TIAKAVEEAGADGLVMINSFGPCMAIDVNTGYPIMGSKAGYGWLSGAPIRPLAIRCIY
                     EASQVVNIPIIGVGGITCGKDVAEMMMAGASAVQVCTEAILKGPSIYGKIAKELNEFL
                     DENGYADVNEIKGLTHKKMAARGFRTHSVAPVVDDNKCIKCKQCVTSCVYEALEVTDK
                     LNIDADKCFGCGLCVTRCPKGALSICYNQVEAMNE"
     gene            92102..93493
                     /gene="hydA"
                     /locus_tag="KR250_000409"
     CDS             92102..93493
                     /gene="hydA"
                     /locus_tag="KR250_000409"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HBH0066378.1"
                     /translation="MGIVLKGGKVVTSEKTYCADVRIEDEKIVDIGLDISKENDEIVD
                     VSDSYVIPGGIDTHTHFDLDTGVTKTADNFETGTKAAIVGGTTTILDFATQAKGCSLK
                     DGLEEWHEKAKDKSYSDYGFHMAITDWNDSVCNEMEDMVKEGVSSFKLYMAYKGSLQV
                     DDGVIFEALRKAEEIGGIIGFHCENGDIICELVDKAKSENNLSPKYHQLTRPAIMEAE
                     ATSRLMKIAEVANSKVYVVHLSCKESLEEVLKAKQRGVDVIVETCPQYLLLNDELYDL
                     DGFESAKYVMSPPLRNKENNEILWNAILDNQIDTIGTDHCSFNYKGQKELGINDFSKI
                     PNGSPGVEHRMSLLYTYGVCKNKISINKMVEVTSTNAAKLFGMYPNKGTIEIGSDADI
                     LVLNPNKKSKIEAKKQLQNVDYTPYEGYEVDCQIEHVYLRGKEVVRDGELICNTPSGR
                     YINRRLTKGAREW"
     gene            93487..96048
                     /gene="xdh"
                     /locus_tag="KR250_000410"
     CDS             93487..96048
                     /gene="xdh"
                     /locus_tag="KR250_000410"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent xanthine dehydrogenase"
                     /protein_id="HBH0066379.1"
                     /translation="MVEFILNGEKVVAKEGKKLLDFLREDMNITSVKNGCSEGACGTC
                     MVIIDGKATKACVFKTDKLAGKNITTIEGLSERERDVFAYSFASQGAVQCGFCIPGMV
                     ISSKALIDVNNNPTEDDIKKALKGNICRCTGYVKIIKAVDLAAKIFRENIDVPKVNCK
                     GLVGENLPRVDAVGKALGYSEYVDDMRIEGMIYGSAVRAKYPRALVKKIDIEEAKAYP
                     GVVAVLTAKDIPGTIKVGHLKKDWDTLIPEGEITRYLGDGIVLVAAETREALEEAKKL
                     VKIDYEELTPLSTTEDALREDAPKIHETGNILVKEHLVRGNAKEKIENSKYVVTKRYT
                     TPATEHAFLEPECAVAGPYEEDGVIIYSTDQGVFATQHECADMLGLPYEKVRVINKMV
                     GGGFGGKEDMSVQHHAALLAYHTKRFVKVQLTRKESIIVHPKRHATEMEFTTACDENG
                     YLTGMKATIISDTGAYASLGGPVLQRLCTHAAGPYNYQDIDITGTAVYTNNPPGGAFR
                     GFGVTQSCFATECNLNLLAEMVGISPWEIRYKNAIRPGQVLPNGQFADEGTAMAETLE
                     AVKEEYEKHPRAGIACAMKNSGVGVGIPDVGRCRLVIKDGKVHIRTSASCIGQGLGTV
                     MTQVVCETASLLPENVVHEVPDTHLTPDSGNTTASRQTVFTGEATKVASNKLKEALDS
                     GKTLLDLEGEEFYGEYESITDKMGCDKENPVSHVAYGYATQVVILDENGKLEKVVAAH
                     DVGRAINPISLEGQIEGGVVMGLGYALTEDYPVVNSVPTAKFGTLGLFKSTDIPEIQA
                     LIVEKNKDLLAYGAKGVGEICTVPTAPAVQNAYYVFDKEFRTELPLQNTPYSKKK"
     gene            96224..97570
                     /gene="rpoN"
                     /locus_tag="KR250_000411"
     CDS             96224..97570
                     /gene="rpoN"
                     /locus_tag="KR250_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HBH0066380.1"
                     /translation="MNFNNSLELTQSQKLIMTTQLKQSLSILNMSKLELEEEIKREAE
                     NNPLVEVEKSGEINWEEYIKDMDRSRRLDRTEISYNPDNEVNLENLVKYSSNLYEDLK
                     FQISLYKLTDKELEVCEYIIDSLDEDGYLRTEEKVIVDTLKIDEELFEKCLISVQQLE
                     PSGVGARSLSECLMIQMASLGIYNEILEEIVTKDLNLIANNKYKEISKKYNMSLQKCV
                     ELINIIKTLDPKPGRTCSVEKSVYIQPDVTVEKIDDEFIVYLNEKDSYQIRINSYYKD
                     ILKNSQSDESAKEFIKERLNSATGLMKNIESRKTTVLKIAEEIVKAQDEFLRKGTKYI
                     KPLKMKDIAEKLEFHESTISRGVNGKYMLTPFGVYEFRYFFSSAIETENNEMASSTSI
                     KKIIKETIKDENKKKPLSDDHISKLLKEKGINVARRTVAKYREELGIPSSSKRKEY"
     gene            98366..99388
                     /locus_tag="KR250_000412"
     CDS             98366..99388
                     /locus_tag="KR250_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBH0066381.1"
                     /translation="MKNLLEIQKKLIPQAIELMERRYSILRQISLSEPIGRRTLSNVL
                     EISERIIRSETEFLKEQGLIDVAVSGMTITKEGSDLLDKLKDIMSDIMGLSKLQDRVR
                     EKLGIKRVILVPGSCDENGSLLKDVTRYGCEYFLGILKDGDIVSITGGSTMLEFSNVI
                     KTDKRYPNSTIVPARGSMGTDVEMQSNNIVASISKKLHSNCKLLHIPDELDESAMKTL
                     SQVPEIKTTLEYIKRTDILVFSIGRADVMAKRRKLPEDKVDEILSKNAVGEAFGYYFN
                     KDGEIVYKLNTVGIDLETVKQVKETIAIFAGTEKVEALLAISNMHKNMVLVTDEESAY
                     KILSLL"
     gene            99448..100455
                     /gene="gap"
                     /locus_tag="KR250_000413"
     CDS             99448..100455
                     /gene="gap"
                     /locus_tag="KR250_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBH0066382.1"
                     /translation="MVKVAINGFGRIGRLALRKMMEQQDKFEVVAINDLTDAKMLAHL
                     FKYDTAQGRFNGEIEVKEGAFVVNGKEIKVTAERNPADLPWAELGVDIVLECTGFFTS
                     KDKAEAHIQAGAKKVVISAPATGDLKTIVFNTNSDILDGSETVISGASCTTNCLAPMA
                     KVLNDKYGIEKGLMTTIHAYTNDQNTLDGPHPKGDLRRARAAAGNIVPNTTGAAKAIG
                     LVIPSLKGKLDGAAQRVPVVTGSITELVCTLGKNVTVEEINAAMKEASNESFGYTEEM
                     LVSSDIIGISYGSLFDATQTKVMEVDGKQLVKVVSWYDNEMSYTSQLIRTLGYFAQLA
                     K"
     gene            100677..101879
                     /locus_tag="KR250_000414"
     CDS             100677..101879
                     /locus_tag="KR250_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HBH0066383.1"
                     /translation="MSMLNKKTIEDIDVCGKKVLVRCDFNVPLQDGVITDENRLNGAL
                     PTIQYLISKGAKVILCSHLGKPKGEAKPELSLAPVAKRLSEMLGKEVVFAADDNVVGE
                     NAKKATEKMENGDVVLLENTRYRKEETKNEENFSKELASLAEIFVNDAFGTAHRAHCS
                     TVGAGEFLQERVCGYLIQKELKFLGEAVANPVRPFTAILGGAKVSDKLAVINELLEKV
                     DNLIIGGGMAYTFLKAQGYEVGTSLLEIDKVEYAKEMMEKAKNKGVNLLLPVDVVMAD
                     HFAPDATPIVTEDANVKEDYMGLDMGPKTIANFVKTIKESKTVVWNGPMGVFEFENFA
                     NGTLSVARAMAELTDATTVIGGGDSAAAVNQLGFGDKMTHVSTGGGASLEFLEGKELP
                     GIAALDNK"
     gene            101905..102648
                     /locus_tag="KR250_000415"
     CDS             101905..102648
                     /locus_tag="KR250_000415"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HBH0066384.1"
                     /translation="MRKPIIAGNWKMHKTIKEALEFVNEVKDKVNSDKVEAVICAPFT
                     LLKDLKEATKGTNIKIGAQNMHFEEKGAFTGEVSPLMLKEIDMDYVVIGHSERRQYFN
                     ETDETVNKKVLKALEVGIDPILCVGETLEQREAGKTKDVCKVQVEKALENVLKDDLAK
                     VVVAYEPIWAIGTGKTATAEDANDVISYIREVIKGLYGELANEVRIQYGGSVKPSNVA
                     EIMGQSDIDGALVGGASLASNDYLDLVNF"
     gene            102657..104189
                     /locus_tag="KR250_000416"
     CDS             102657..104189
                     /locus_tag="KR250_000416"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003228330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HBH0066385.1"
                     /translation="MMKKPVALIIMDGFGYNKDVKGNAIAESKTPNLDRIKKEYPNTL
                     INASGLDVGLPDGQMGNSEVGHTNIGAGRIVYQDLTRITKSIKDGDFFTNKVLCEAMD
                     NAKENSLHVMGLLSDGGVHSHIDHLKAIIKMAKDKGVQKVYVHAFTDGRDTDPQSALE
                     YAKEVQASMDEIGVGEFATVSGRYYAMDRDKRWERVELAYNAMVRGIGEKANSIEEAI
                     QNSYDDGKNDEFIMPTVIMKDDKPVGSIKENDSIIFFNFRPDRARQITRALVCEEFDG
                     FKREDIKNFFVCLTEYDITIENVHIAFGPQSLANTLGEYLAKNGKTQLRAAETEKYAH
                     VTFFFNGGVEEPNKGEERLLIPSPKVATYDLKPEMSAYELTDKALDKLGEDKFDFIVL
                     NFANPDMVGHTGSIEAAIKAVETVDTCVGRLIDKIVELGGSAIITADHGNAEYMLDPE
                     TGKTVTAHSINPVPFIVVGQEYESAKLLDGGRLSDIAPTVLDMMKLEKPEEMTGHSLI
                     SK"
     gene            104777..106069
                     /gene="eno"
                     /locus_tag="KR250_000417"
     CDS             104777..106069
                     /gene="eno"
                     /locus_tag="KR250_000417"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HBH0066386.1"
                     /translation="MSVIELVYAREVLDSRGNPTVEVEVVLEDGAMGRAIVPSGASTG
                     AFEAVELRDGDKGRYLGKGVETAVANVNEIIAPEIEGMDAFDQPAIDAIMIELDGTPN
                     KGKLGANAILGVSMAVARAAADEIGLPLFQYLGGVNAKQLPVPMMNILNGGEHADNNV
                     DVQEFMILPVGACCFKEGLRMGAEVFHSLKKVLGEKGLACGVGDEGGFAPNLGSNREA
                     LELIVEAITKAGYKPGEDVMLGLDVAATEMYNKETKKYVLAGEGKELTAAEMVALYED
                     WSNNFPIITIEDGLDEEDWDGWKLLTEKLGNKLQLVGDDLFVTNTERLEKGIENGVAN
                     SILVKVNQIGTITETLDAIEMAKRAGYTAVISHRSGETEDSTIADLAVAVNAGQIKTG
                     APSRTDRVAKYNQLLRIEEMVGEQARYCGLKSFYNLKK"
     gene            106662..106886
                     /gene="secG"
                     /locus_tag="KR250_000418"
     CDS             106662..106886
                     /gene="secG"
                     /locus_tag="KR250_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HBH0066387.1"
                     /translation="MSNILMGVQVVLGILLVISIMPQDSKNALPSEFGGEGSQAYFKP
                     KGKQAFLARVTKITAVLFFINALALLIVKK"
     gene            complement(107270..108208)
                     /locus_tag="KR250_000419"
     CDS             complement(107270..108208)
                     /locus_tag="KR250_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HBH0066388.1"
                     /translation="MENLILSINVVLPLFLTMSLGYVLKKLGLFDTHTLNKMNNVCFK
                     SFLPLLLFFNIYNTDLDGSLNFRLMAVAVIIIFSIFLILVFIIPKIEKDNKTRGALIQ
                     GVFRSNFVIFGIPVTISLFGDKSIGVTSLLIAVIVPMFNVLSVVILEIFRGGKVNIKH
                     MIKSVLTNPLIIASFIGLTMLILNINIPTFLHKTIGDISKIATPLSLIILGGSFSFSA
                     IKGHINPIILGVCGKLILVPAIFLPISIFLGFRNVELASLMIMLSAPTAVSSFTMAQQ
                     MDSDSELAGQIVVFTSGFSIITVFLIIFILKQNNLI"
     gene            complement(108196..108594)
                     /locus_tag="KR250_000420"
     CDS             complement(108196..108594)
                     /locus_tag="KR250_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBH0066389.1"
                     /translation="MIKKIEHVVITNNNIENYINFYKKFGFSSKEMSKNYKLNARNIK
                     INLNIKGYKFLPYVKNLQASRDNLCFKINTNIDEPKKYLEQNEIQIKRGIVECTGVFG
                     NMKSIYLKKTNGNLIELSNHRITESKPWKI"
BASE COUNT        41828 a        12818 c        19100 g        34859 t
ORIGIN      
        1 gttcccccta aataagattt agatgtatag tattaaaatc aaaaagtata aaataaaatt
       61 gatgcttaat catagataaa tattaataaa atatacaagg ggattgtcta ataaaaagat
      121 aatctccttt tttacaaata attctaaatt gtgatataaa tctaaaaatt ttatcttaac
      181 atttaaccat ttaatgataa taattgatat aataaatata aattaaatat atggtgtact
      241 tattttatgt ttttagcaag aaatatagca tttaaaagat aaattgtgca acttaaatca
      301 aagtttaaga tgaactatta acatagatat attaaaaact tttgtaatgg agaatatatg
      361 tgatatgaat aataaagtta aggttgcagt attagataca ggaatagaca aagaacatga
      421 ttacttaaaa gataatttag ttggaggaat tgcttttgaa tgtatgcatg attatatctt
      481 tatatcagat aaatttgatg atgaagatgg tcatggaact gcttgtgcat caataataaa
      541 gaaagagtat gaagatgtag agttatttgt aataaaaatt ttgagaaaag atggtattac
      601 aaatataaaa gttcttgaag aagcattaag atacctttta gacactaaca taagattaat
      661 taatttaagt ttatcagtca taggagtaga aagtgttaag ggcttatttg aagtttgtta
      721 tgaattgttt agaaaaggaa aaataatagt atgttcactc gctaatgatt ttgacttaag
      781 ttatccagct atgtttaaca atgttatagg tgtaagagga tttactttag atggagatga
      841 ttcattttgg tacaacaaaa aatatgatgt tcaatgtgtt atggatagca attcatatat
      901 aagttgtgat ataaataatt cctatagatt accaccaaaa tgtaatagtt atttatcagc
      961 aaagcttact ggtaaaatag ctaagatatt atcagaaaaa ctaggcataa caatatctga
     1021 tttaaatgat aagcttgaat cattagcaac tagaaatcat tgggatagtt gtgattttga
     1081 tgagtgtagt agaattgtaa actttaaatt ggatttgtat gacaaagaaa atgctctttt
     1141 tatgaaagtt gctgacgttg ttagggattg cttagatatt gaagagaata atgaaaaact
     1201 ctttaagggt agccttttta ataaggagat aggcttggtt tatgataatt gttttaatct
     1261 gttgaaaaag ctagagcata gatttgacat aaagtttaat tatatggaca tctcaaaata
     1321 tgacttagtt tcaatatata cactgactga attagtggaa agatataaaa acttataaat
     1381 aaagatttgt agctaaattt aatgtagaag gtattaaatt atatgtattc tttgtcggta
     1441 gattaaaagt aaaaaatata ctataattag atagcataag agaaaatgcc aatacagggg
     1501 gagttagata atgaaatatg aagaagcatt agagtacata tcacaaacta ataaattcgg
     1561 tataagatta ggattagaaa atatcggaaa gttattagaa ttattaggaa atccacaaga
     1621 aactttgaat ataatacatg tagctggaac taatggaaaa ggttctgtgt gttcttttgt
     1681 ttctaatata ctaagagagt gtggatataa agttggttta tatacatctc catatttaga
     1741 aacttttact gagcgtataa gagtaaatgg acaaaatata ccacaggatg atgttgctag
     1801 aataattgag ttaataaaag aaaaaataga gattatggta aaagaaggct atgcatatcc
     1861 aacagaattt gaagtggtta ctgcaatggc attttattac tattcagaac aaaaggtaga
     1921 ctttgtggca ttagaagttg gtttaggtgg cagatatgat gctacaaata taataacaaa
     1981 atcacttgta agtgtgatag tatctataag tttggaccat acaggtatac ttggagatac
     2041 aatagaaaaa atagcgtacg aaaaggctgg aataataaag gaaaatggag tagtactagt
     2101 atatgaccag actgatgaag caaaggatgt tataaagtca gtttgtaaag agaaaaaagc
     2161 taaatatata gaagttgatt ttgatgatat aaacataaaa aaatcagata taaattctca
     2221 aatatacgat tgtactgtaa tgaaagaaac ttatagagac cttgaaataa aattgatagg
     2281 cgaacatcaa ataaacaatt ctatcttggc aattagtgta ataaaatatc taaaagacat
     2341 aaataaatta gcaaatataa atgaagaatc tataagaaaa ggacttataa atactaaatg
     2401 gccaggaaga atagaaaaga taaaagaaaa tccaattttt ataatagatg gtgcacataa
     2461 tgaagatgga gcaaaatctt tggcaaaagc acttgataag aattttaaag gtagaaaatt
     2521 aaccttatta ataggtatgt tagaagataa agatattgat agtgttttag agatattgtt
     2581 gccacatttt aataaggtaa taacaacaac accaagtaac ccaagagcca taaattcaga
     2641 tatattaaga gaaaaagtat taaaatatgt agatgatgta acatcaaagc atgagataga
     2701 ggatgcagta aattatacat tagaaacatc aagtgaggat gatattataa taagtgcagg
     2761 ttcattatat atgattggta cagtgagaac tttagtaaag aaattataat tttataaaat
     2821 aagtatacaa ataaaaatat aaaaaagagt tgtctaaaaa atggacaact ctttttatat
     2881 aaaataagtt tttaataatt ttactgatgt ttatataagt aattatatag attgacgtat
     2941 agctttagat aattggtctg ggcaagaagt accttttcca ccacagtcaa taccatttaa
     3001 tttatcagca acttcaagag cattttggcc aattattaag cttctaagtc ctgataaatt
     3061 accattacat cctcctataa attcagcatc aacaatcaca ttattttcat caacttcaaa
     3121 aatcatttct ctacaacata caccacttgg attaaatgtt actttcatta aaaagacctc
     3181 cattaaatat tatttctttt tttaacatta ataataatac caaatatatg cgtaataatg
     3241 caactataaa gcataataca agctaatgaa ataatatatt attgtagtat atgtattgta
     3301 gagaggaggg gttatatgaa aatctatatt tctaactact tatcaaagag tattagcata
     3361 gttgattatt ctacacttga actagaaaaa gaaatagtat tagaagataa tatatatcca
     3421 caccattttt gcatagaaaa agaaaaaaat ttaatataca tacctagttc aagtaatggg
     3481 atactctatg tcttagattt aagtaatgat aaaataattg atactgtctc tataggagga
     3541 agtcttagtc aagttgtact tagcgataat gaattgtttg tggcaaatga agattctaat
     3601 agtatatatg tactagataa aaacacatta aatccaatag gaattattgg agttgataat
     3661 atgccacatg gatttgactt tgatagagaa agcaaaaagt tatatgtacc gtgtataaac
     3721 tcgatagtat gtatagatac aattaataaa aatatacaaa agaaaataaa tatggacttt
     3781 agagcttggc atatagaact tgataaacaa aagaaagaaa tgtatatatc aacacttgat
     3841 ggaaagctta taataataga tgaagtaagc atggatataa aaaaggtatt atatgaattt
     3901 ttattaccag tagaaataag atttaattat agtggcaaaa aagtttatgt tgctgatttg
     3961 ggttataata atgtaagaat tttagattat acaacgagta gatacattgg aaatattgaa
     4021 ataaatggga ttccccaagg attagaaata tctaaagatg aaaagttact atttgtatca
     4081 gatacacaag aaaattcagt aaaagtttat gaaactgcaa ataacaaatt aataaaagaa
     4141 atcaaggtag gaaaagagcc tacaactata atttgtttgt aggctttaat gtatgtctct
     4201 tataagtaaa tcgataactt cggcatacat ataagatgca ttatttttcc agttattgca
     4261 aataagtttt gcttgtttct ctgatgaaac atttagattt aaatctatta agttagactg
     4321 attttctatg actctaaggt tgacaataaa ttcattatca ctttgtttta caaaactaga
     4381 ctttacttga gtatctgcta gtagattttc tttgttagaa tctatatact tgtctatttt
     4441 ttcagtaaca ctagtaggta ttcgattaaa gaagtattct agactttcta cgcctctttg
     4501 agtaagagca taatattctc tgtcagagtc tttatatgta gtcaaaaact tagattccat
     4561 aagctgagat aaaagctgtt gtaatgaaaa ataattcatc atctcagtct ctaatactac
     4621 ttgagttatt tgagaattag ttaaatccat tttaattttt tctaatatat ataaaattaa
     4681 aagtttgtga taggctaatt cttcagacga gttttcaaac ataaatatca tcctccgtca
     4741 aactatttct atatataata tattaaacta atttgtacaa aattaaaaga acaatttgta
     4801 ttgtattatt aatctttaat aattcatata aaaacaattt tataaataaa tatagaaaaa
     4861 cagggctgct aattagcagc cctgttgata cccaggtgtc aaaatttact atttattttc
     4921 cagccatttg tctttcagcc atttcaacta atttcttagt catatatcca ccaacatatc
     4981 cattttctct agctgttaag ttacctttgt ctatgttttc atagttagat aaacctattt
     5041 cgttagctat ttctaatttc atttggttta aagcagcttt tgcttctgga actactgttc
     5101 tgtttgaatt tgacatagta aatttcctcc tcaaataatt agtttatgat gtctaacaac
     5161 acttttattt atgtgatgtt aatactaatt tctccaaaat aaaaataata tatacagaaa
     5221 aataaaggta aaaatgtaaa aaatataaaa aaattaaata aatgagtatt ttagagagtt
     5281 taattaggtc aatataggtt ttattttttt agttattgtc ttataaatta aatattacag
     5341 tacttttttg agctgaaaaa aaataaattt taaatataga agaaattatt tcaaaggggg
     5401 aatagtatga aaagagaagt taaaaggact aaagctttga tatatagtgt tttatttttt
     5461 tcattattat tagtgttaat atttgttttt gtttatggaa taaaatacat atgttctaaa
     5521 tctgacaatt caaaaatgcc tatatataaa gttgatacaa atgagaaaaa aatagctttg
     5581 agttttgatg tagcatgggg aactgataat atggatgata ttttaaaaat tttagataaa
     5641 cataatgtaa aagcgacctt ctttttagtt ggtagttggg tggatgataa tgaagaaatt
     5701 gtaaaagcta tagatgaaaa aggacatgaa attggaaatc attcaaatac tcatgccaat
     5761 ttaaaggaaa tatcaaaaga agacataaca aaagagatag aaactacatc agaaaaaata
     5821 tataatataa caggaaaaag gactaattta tttagacctc cttttgggga cgtaaacaat
     5881 aaagctatgg acatttgtag tgatttaggt tataaagtaa ttaagtggga tgtagattct
     5941 atagactgga aggagcttgg accaaatcat gtaattgaaa aagtaataaa agaatctcaa
     6001 cctggttcaa tagttttatt ccatgctaat ataaatgatg tagataatta tttggatact
     6061 ataataagtc gactaaaaaa agatggatac agtctagtca aaatatctga tttgttgtat
     6121 aaagataatt atgtagttga ttccaatgga gtgcaaaaaa ctaagaatta ataagtaata
     6181 aatgtatatt ttaagtaaag ttgtgataaa ttatatagta tatgttagaa aagtgtcgat
     6241 aaaaagacgg tgtctatttt ttgacacttt ttatacatgt atgcaatatc aataattata
     6301 gattaaattg attaaaaaag tgtctaataa ttgacttttt tttgatgggt ttaaaaattt
     6361 agaagaatta atgaatgctg taagacagta tttccaaagg gtataagttt gtttaaaagt
     6421 tggcatagaa attgctttgt taataagcga tatgttctat agaaataaac aatatgttaa
     6481 gaccttataa tagaaaaata gcattaacat tatttgaaca gcttataaaa aaattaatat
     6541 acttaagggg gcgtactagt tgaacaaact atattctatt ttattaaaac aacacgttgg
     6601 tggtccagat aagccagtag taagtgttgg agatgtagta aaaaaaggaa ctctaatcgc
     6661 agaaccaact ggattagggg caaatattta tgccagtgtt agtggaaaaa taagtgaaat
     6721 aaatgaccaa gcgatagtaa ttgaagctga tgaagtacaa gaagatactt ttgaaccatt
     6781 aaaaggagaa ggtatacttg acttaattaa agaagctgga gtagtaggaa tgggtggagc
     6841 aggattccct actcatgtaa aattaaacat agatttaaat ggtggaacaa tattagcaaa
     6901 tgcagctgaa tgtgaacctt tattagctca taatataaaa gaaatagaag aaagaccaga
     6961 aatagtttat caaggtataa aatatgctat ggaagttact aatgctggaa aaggtatgct
     7021 agcaataaaa agtaagcatc caaaagcaat agaagctttc aaaaaagtaa taaagccagg
     7081 agacaacata gaagttgcag aactagttga tatgtaccca atgggagaag agagagcaat
     7141 agtaagagat gttctaggaa aattacttga gccaactcaa ttaccttcag aggctaatgc
     7201 agtagtaata aacgtagaaa cactaactag aatagtagag gctgttgagc aaaaaagacc
     7261 agttatatct aaaaatataa ctgtagttgg tcaattaaac agtggaaaag aatctatagt
     7321 gtttgaagat gtgccaattg gaacaactgt cggagaatta attgaaagag ctggtggaat
     7381 aaaaggtgaa tatggtgaaa taatcctagg aggacctttc actggtaaag ctactacttt
     7441 agatgctcca ataactaaaa ctagtggtgg tataatagtt actatgccat ttgttaatga
     7501 aaagagaaaa atgggtcttt tagtttgtgc atgtggacct aatgaagaaa gaatgagaga
     7561 tatagcaact aaaatgggag ttactgatat agtatcagtt caaaaatgta aacaagctca
     7621 agaaattaaa ggtgcattaa aatgtgaaaa tccaggacac tgtccaggac aagctcaaaa
     7681 atgtattgaa tttaagaaag ctggagcaga agttatatta ataggaaact gtactgactg
     7741 tagtaacaca gtaatgggtt ctgcaccaaa attaaaatta ggtgtatatc atattactga
     7801 ccatgtaatg agaacagtta atcacccatt aataagaaga ataaaaggtg acgtaagatt
     7861 aaaaataaaa taatagataa atagaagtta gacatcttta gaagtaaaac ttaaaagtta
     7921 cttctaagga tgttttttaa tgtattttga gggcaatata tatattgtaa aataaatgga
     7981 aaaaatagac aaaatatgct agatattgta taatgataac aatagctatt acaattaata
     8041 ttggacaaaa ataatgatac tcaagtatgt ggtttgtatt aatatgcata ttatattaag
     8101 tcaactatcc gatagagtaa aaggtttttg tgatatttga agatactcta tagtatcttt
     8161 tttattataa aaactaattt ttgtctttta ttagataagt atctaggata ctaatttata
     8221 ttttattgag ttgttaaaaa agtgcactta aaaagttaaa aaagaaaaat taaaaaccta
     8281 tttatagaaa ctgtgaagta tatcttaaac ctgggcactt aaaagatata tggagttagt
     8341 agtgcaacct gctataaata taaataggag taacttttaa ttgtcaaaaa tatataaaat
     8401 aaatacataa ataagtaatt aatagaagat agatataaat atatttttga aatgctccct
     8461 gcattcataa cgagtgtaga gtggcagata tctgcccaaa actcctttaa ttgctggaag
     8521 ttcctaaagc taactaaacc acaacataat catgaaataa ggataagtgt gatagagtgt
     8581 gcatttaata agcacttgag gttacgaaag tagaaaaaat tagttagata gcataaggtt
     8641 aaatcctaag tgccttgaaa atggataatc agcaggtaag cttcgaaaag aagaaacttc
     8701 aacgactaga gagaaatctc gtacctacaa gtgtggggaa atggggagta cctaatttat
     8761 ggttaggtaa tgatatagtc tgtgcttaat agaaatatta agaagttcat aggagaactg
     8821 gttaggagta acgaccctaa ttgaacacac taaagatgta ttattgaata agattcatat
     8881 aatatttaat aattttaatt atattaagtg aatacaatat atgtttttag tatcaggaga
     8941 attttgaaaa caaaaattaa aaaatcaagt ataatcagtt tagtaattgc ttttagtatg
     9001 gtatttactg catttacacc aatagtatct tatgctgacg aagtgactag caatgataca
     9061 atacttaatg gagaagaaca atcaaatggt cagacatcag atgtagagaa accatcagac
     9121 ggtcaggtac cagatgggga gaaaccatca gacagtcaag taccagatgt agagaaacca
     9181 tcagacggtc aggtaccaga tggggagaaa ccatcagaca gtcaagtacc agatggggag
     9241 aaaccatcag acggtcaggt accagatggg gagaaaccat cagatggtca gataccagat
     9301 ggggagaaac catcagatgg tcagatacca gatggagaga aaccatcaga tggtcagata
     9361 ccagatgggg agaaaccatc agatggtcag atgccagatg gagagaagac atcagatagt
     9421 cagacgccag atgtagagaa accatcagat ggtcagatgc cagatgggga gaaaccatta
     9481 gatgagcaaa ctccagaaga agaaaaacca ttggaagaag aaatagttat agaggaaatg
     9541 agtttaaaac aagatattga taaaattctt gatatgactt tatctcaaat aaataaaata
     9601 gtatataact tttgggaaga tgaagaagat gtaaaggcag atgaacaatc agaaattaac
     9661 caagttttta cttcagaaga ctcatttatt agcttatggt atgacaaaaa agcaaaagta
     9721 aaaaattctt gtttactaaa agaaatagat ggttcagata gaccttatca tgatttaaga
     9781 tttgatgata ttacaaagac tttaactttt gactatgtaa taaagggtct tgtaggagca
     9841 agctcaaaag acgacaaata tgtaatagaa ggtgaagatg gagagcaaac tgcattttgt
     9901 tacaataatc atttaagacc accaagtagt aatggaaaga gtccatattt accagcagaa
     9961 gattttaatg gtcaagaaaa ccaaaatgaa gaagcagtta aatctatact atatgcaggt
    10021 tctgaatttg atggatttgg atataagcaa caatttaatt taggtggaga agaaaatgaa
    10081 ttgatgactt actcagctac tcaaagtgca atttggatta ttcttggaca aatggatgaa
    10141 caagaaagac ttaagcagta tcaaggttct ataaaccttt gtgataaact tatagaaaga
    10201 gcaaaaactg aagaggaaag agctgagtat cagaagaaga aagaagtagc gataaatata
    10261 aaagcttatt tagaagcatt gttaaaagca ggtcgtgaag agttaaagcc aaataataca
    10321 ggaaaaccta gtttgagtaa tggtttaact aagattaact ttgaaaaaaa tgaagatggt
    10381 acttatgaaa cagaagctgt agccttagta ggttatagtg gtgtagtaaa gttaaaacta
    10441 ccagatggag taactgctta tgatggagat ggtaatataa taggtacagg cgaagtagaa
    10501 atttcaacgc aacagaaatt taaacttaag agtgttggaa aaccagatgc caaagctaat
    10561 atttctgctg tatcttatga ttatattttt ccaaaagcta ttcaatatta taaggcagtg
    10621 cttgatttag gacaaaaaga ccattcatct tcattaccat ctagcaagca aaatttatta
    10681 agttatacaa tagagaaaaa gaatggtcaa gaagttaact ttaatatagg tttaccaaca
    10741 gatgatgatg atgttgtaaa tccaccagtg ccaccaatag atgatgatgt tgtaaatcca
    10801 ccggtgccac caacagatga tacaaatgga cataagccaa aaccatcacc accaatagat
    10861 gatacagtaa taaatccacc agtaccacca atggatgata caataataaa tccaccagta
    10921 ccaccaacgg aggatgcagt gttaaatcca ccagtgccac caatggagga tgcagtgcta
    10981 aatccaccag tgccaccaac agaggataca gtgttaaatc caccagtacc atcagtaagt
    11041 gatacagtgg aaaaaactcc agaactatca agagatgata caataataaa atcaccaaaa
    11101 acaggtgatg aaactcagct aatgtcatat gtatttatta gtgtcattgc aatttgtgga
    11161 ttagcgtatc aatgtaaaat aaaaagaaat taatctagat tattattaag tttaaaataa
    11221 aaagtctcaa attgatttat aagtcatttt ggggcttttt tatgtaaata taatatgcaa
    11281 atatagtttg tttaaaaata tctattttaa ttatattctt aaatttatta ttggatacag
    11341 gcattcatta attgtttgat gaatagaaaa atgttttgaa ggtgaaaata gtattgaaaa
    11401 aataatatac taagggtata attcatttga tttcatttgt ataaatttaa ttataaattt
    11461 taaagtgatt atataaattt aaatgctaat tacttaataa attttaataa taattattta
    11521 ataaataatt attataatat gctagaataa atatatataa ataagttttg atgaggtact
    11581 actataaagg ggggacttta tgttttcgat accagaagta aaaaaagtag aagaggtaat
    11641 ggatacaaaa tttacaacta tagacgaaga cacaagaatt gaagatgcaa taaaagaaat
    11701 gataaaaagc aacacaaaaa ctctaatggt aatagattca tctgaccaat taaaaggcat
    11761 tatatccatg acggatatac ataatcttta tgagatgcat aaaaagtatg aaggtcaacc
    11821 agtaaaactt ataatgaaga aggatgtaat atatgtcaac gaaggtttaa ccttagatga
    11881 atgtagagat ataatgatat taaaaaatat agggattctt cctgttctta gagataataa
    11941 aattattgga gttctaaaac aagagcacat tagggactat ctatatatgc atttagaaga
    12001 ttatggatta actttgaagt atattatagg tcaaattaaa gaaggaattt gtgctataaa
    12061 taacgaaggt gttgttatct tgtggaataa gtttatggag gaaaggtatg atataaagtc
    12121 tgaagatata gttggtcgac cgatgaatga atttttggaa aatactattt cagaaaaagt
    12181 actaaatagt aaagttggaa tgagtgactt gtattttaca gataaaaaag aaaatatgta
    12241 tgcattagtt catgcaaatc ctatatttta taaagaagaa tttatcggtg ttgtatgtac
    12301 agaagttgat gttacagaag ctaagatatt agctcttgaa cttgaaaaag taaatgatac
    12361 attaaaatat ctaaagaacg aagtcaaaaa tttatctaaa ggtagttttg ataagatttt
    12421 aggtaaaagt tataaattgg aaaaatcgaa agcgattgca aaacaagtgg ctagaacaaa
    12481 tagtagtata tttatttggg gcgaaagtgg tacaggtaaa gaagtatttg caagagctat
    12541 tcatgattat agtgagagaa aagggcaatt tatacctgtt aactgtagtg ccataccaaa
    12601 tgagttattt gaaagtgagt tttttgggta tgaatcaggt gcatttacag gagcaagtaa
    12661 aaagggtcga ataggtatat ttgaacttgc aaaagatgga actgtatttt tagatgaaat
    12721 tgccgattta cctcttagta tgcaagcaaa gttgcttaga gtattacagg aaaaagaaat
    12781 aagaagagtt ggtggagata ccacaataaa aataaatcct agaataatat cagctaccaa
    12841 taaagatttg agtaaaatgg taaaggcaga aaaatttaga gaagatttat actatagatt
    12901 aaatgttgtt gaaataaaga ttcctccact tagagagaga aaagaagaca taggattatt
    12961 ggttcatagc tttttagagg agatatgtaa acaaaataat aagcctgttc ttacaattag
    13021 caaagatgta atagatatat ttcagaatta tagatggaaa ggtaatatta gagaattaaa
    13081 aaatacgatt gaaaatattg ttgtattgtc acaaaattct aaaatagatg tggatgatgt
    13141 accaagttat atgatggatt ctacaaataa tagtactgaa gaagaagagt atcctttaga
    13201 tttaacgaag gcaactcaaa aaattgaaat aaagaatata accaaggcac ttaaaatgtc
    13261 taatggaaat aaagctaaag cagctaagat attaaatata cccagaacaa ctttgtatta
    13321 taagatagac caatacaaaa tagatgtgtc taaaatatga cacatgacta aattttgaca
    13381 aaatgataaa ctttaattta aaacctagta aagccaacac ttgtataagt gttgtgtata
    13441 tagatgtgtt aaaaaaaatg aaaaagtgtt gattaattga cacttttttt gtttgagaaa
    13501 atgtttacaa atacaaaaaa taacatattt taaataaata tttatataat atttggctaa
    13561 aaatagcgat agatgaagaa tctattatag aaaaaatctt tttgttaata agttggcata
    13621 ggaattgctt ataatataaa gtgactttaa gtgagaaaaa aataatatta atcataacaa
    13681 attatgtagg ttttaattta cttaatttct cagttaaggg gttacctgtt gaggaattga
    13741 gatttaaaat aataaataag aaaataatgt aaggaggtat tttatactat gtcaataact
    13801 ttagaaacag ctcaagccca tgcaaatgac ccagcagttt gttgttgtag atttgaagcg
    13861 ggaacaatta tagcgccaga aaacttagaa gatccagcaa tatttgcaga cttagaggat
    13921 tctggattat taacaatacc agaaaatgga ttaactatag gtcaagtact aggagctaag
    13981 ttaaaagaaa ctttagatgc actttctcca atgactacag ataacgtaga aggatacaaa
    14041 gcaggagagg ctaaagaaga agtagtagaa gaaacagtag aagaagcagc tccagtatca
    14101 gaagcagcag tagttccagt aagcacagga gttgcaggtg aaacagttaa aatacacata
    14161 ggtgaaggta agaacataag cttagagata cctttatcag tagctggtca agcaggagtt
    14221 gctgctccag tagcaaacgt tgctgctcca gtggcaagtg cagcagcaga agtagctcca
    14281 aaagttgaag aaaagaaact tttaagaagc ttaactaaaa aacactttaa aatagataaa
    14341 gttgaatttg ctgatgaaac taaaatagaa ggaactactt tatacatcag aaacgcagaa
    14401 gaaatatgta aagaagctaa tgaaactcaa gagttagttg tagatatgaa gttagaaata
    14461 ataactcctg ataaatatga aacttacagt gaagctgtat tagatataca accaatcgct
    14521 actaaagaag aaggcgaatt aggttcaggt ataactagag ttatagatgg agctgtaatg
    14581 gtattaactg gtacagatga agatggagtt caaataggtg aatttggttc ttcagaaggt
    14641 gagttaaata ctactataat gtggggtaga ccaggtgctg ctgacaaagg tgaaatattc
    14701 atcaaaggtc aagtaacaat aaaagcagga actaacatgg aaagaccagg acctttagct
    14761 gctcaccgtg catttgacta tgtaactcaa gaaataagag aagcattaaa gaaagttgac
    14821 aactctttag tagttgatga agaagtaata gagcaataca gaagagaagg taaaaagaaa
    14881 gttgttgtta taaaagaaat aatgggacaa ggtgcaatgc atgataacct aatattacca
    14941 gttgagccag ttggtacatt aggagctcaa ccaaacgttg acttaggaaa catgccagtt
    15001 gtattatctc cacttgaagt attagatggt ggtatccatg cattaacttg tataggacct
    15061 gcatcaaaag aaatgtcaag acattactgg agagagccat tagtaataag agctatggaa
    15121 gacgaagaaa tagatttagt aggtgttgta tttgttggtt ctccacaagt aaatgctgag
    15181 aaattctatg tatctaagag attaggtatg ttagttgaag ctatggaagt tgatggagct
    15241 gtagtaacta ctgaaggttt cggaaacaac catatagatt tcgcatctca catagagcaa
    15301 ataggtatga gaggtatacc agtagttggt gtaagtttct cagctgttca aggtgctcta
    15361 gttgttggta ataaatacat gactcacatg gtagacaaca ataagtctaa gcaaggtata
    15421 gagaatgaaa tattatctaa caacacttta gctccagaag atgctgttag aataatggct
    15481 atgcttaaaa atgctataga aggtgtagaa gttaaagctc ctgaaagaaa atggaatcca
    15541 aatgttaaat taaataacat agaagctata gaaaaagtta caggagaaaa aatagtatta
    15601 gaagagaatg agcaatctct accaatgagt aagaagagaa gagaaatata cgaaaaagac
    15661 gaaaactaaa atttagatac taactaagat ttagacaata acataaattt agatccaagt
    15721 ttaaaacagt atagagaggt agaattataa tggataggtt aaaatatatc tcatcagaga
    15781 ctttttatga aggtattata gttgacatca aaggcggtgg agtgactata gacctaaaag
    15841 gtagacttgg acaatttaaa atacctaata gaatgcttat aactgactac gaacttaaga
    15901 taggtcaaga agttggattc atgctaagct atcctgaggt attaagtcca gagcctaatc
    15961 aagagtatgt agaaaatatt aggagagaaa aagaaaaaca agcagaaatg caatcaaaac
    16021 aagaagaaaa ataataaata atcgtttgaa aatagaaggg agaggaaata tatgagcctt
    16081 acaacagtac aaggacttca atctgaaata ttcgttccaa taacacctcc tccagtttgg
    16141 actcctgtaa ctaaagaatt aaaagatatg actatagctt tagctacagc agctggtgtt
    16201 catttaaaat ctgacaagag atttaatcta gcaggagata ctacatttag agctatacct
    16261 aatacagcta cagttgatga aatgatggta tcacatggtg gatatgacaa tggagatgta
    16321 aacaaagata taaactgtat gttccctata gatagattac atgaattagc tgcagaagga
    16381 tttatcaaag gagtagctcc tatgcactat gcattcatgg gtggtggagg aaaccaacat
    16441 gtcttcactg aagaaactgg tcctgctatc gctgctaaac ttaaggaaga gggagtagac
    16501 ggtgtagtta tgacagctgg ctgaggtact tgccatagaa ctgccgtgat cgtgcagaga
    16561 gcaatagagg aagctggaat acctacaata ataatagcag ctcttcctcc agtagttaga
    16621 caaaacggaa ctcctagagc agttgctcca ttagttccaa tgggtgctaa tgctggtgaa
    16681 ccacataata tagaaatgca aacacatata ttaagagata cattagagca attagttgca
    16741 ataccatctg ctggtaagat agttccatta ccatacgaat ataaagctca cgtttaaaat
    16801 gtaatttata tttgaaaata tagaaccctt ctcctacacg gttaggagag gggttttaag
    16861 aacaaaggtg gatattatgg aagaaaaaat aatgagaaga cttgttataa aaccgtttca
    16921 tatgaatgaa gttaatttcg gttcaaaaac ttctataaag aaagatgtac ttacaataga
    16981 tttatcaagt atagatgaaa taaaagaaag agaagagtta ataacagaca taaaagtaga
    17041 cattataaaa cctggagatt atgacagaga aattaatact attatggaca ttataccaat
    17101 atcaacaaaa gttttaggca gactgggaga gggtattaca catactttaa cgggtgtata
    17161 tgtaatgtta actggtgcag atgaagatgg aaatcaaatg catgaatttg gttcttcaga
    17221 aggaattttg aaagaccaaa tggtatttgg gagatatgga actccatcag ttgaagatta
    17281 tataatccat gtagatgtaa ctttaaaggg aggtttacct tttgaaagaa cacttccact
    17341 tgcagcattc agagcatgtg atgattttat acaagaaata agagcgtctt tgaaaatgga
    17401 agacggaaga aatgctactc aagttcgtga atattttgac aaaattcgac caaatgcaaa
    17461 aaaggttgtc atagtaaaac aaatagcagg tcaaggtgct atgtatgaca atcagttatt
    17521 ctcaaaggaa cctagtggat ttgaaggagg aacatcaatt atagatatgg gaaatgtacc
    17581 tatgattata tctcctaatg aatacagaga tggtgccctt agagctatga cttaagagag
    17641 gtggatatta tgggaatagg accttcaaca aaagaaacat ctcttcatca ctttagagac
    17701 ccacttttag acatagttga aagtgataag gatgtagatt tacttggagt aatagtagta
    17761 ggaactccag atggaaatga aaataaaacc tttgtgggac aaagaacggc cgcttggctg
    17821 gaagccatga gagtagatgg agctatagtt tcatcagatg gttggggaaa ctcacatgtt
    17881 gactacgcta acacatttga ggaaatagga aaaagagata tacctgtagt tggagttaca
    17941 tttaatggga ctcaagctaa gtttgttgta agcaatcaat atatggatac catagttgat
    18001 atgaacaaat caaaagaagg tattgagaca gaagtagttg gagaaaacaa cacaaatgaa
    18061 atagatgcta aaaaagcttt ggcattttta aaacttaaaa tgagaaaaca tggataaaaa
    18121 atttaagtaa gggaatgagt tttgtgggaa tttctacatc aatgaaaaac acatcacttc
    18181 atatatttag agacccgtta ctagatttga tagataatga ttctgacata gacttgttag
    18241 gagtggttgt agttgggaca tcagagcaca atgaatggaa aacattttta gcaactagac
    18301 ttggaagatg gattgaatca ttgcgacctg atggagtgat tataacttta gatatggcag
    18361 gaaaccagca cattgatttt acaaatgcta tagcagagtt tgtaaaatca gatataccta
    18421 cagtaggtct tactattatg ggagcagatg gattagtaat taccaatcca tagttggata
    18481 aagctaccat aattgattat aaaaagacaa caggctacat agaaacagaa gtagttggag
    18541 acaatcacat ggatgaagtt gatgttaaaa aagcattggc tttcctaaaa ttaaaaatgc
    18601 gtaaagacgc aaaataaacc taagggggaa tttgatatga aatttagcag aagtatacaa
    18661 gctatagact ctcatacagc aggtgaagca actagaatag ttgtaggtgg aatacctaac
    18721 ataaaaggaa attctatgcc tgagaaaaaa gaatatttag aggaaaatct agattattta
    18781 agaactgcta taatgttaga gccaagagga cataatgata tgtttggttc tgtaatgact
    18841 caaccttgtt gcccagatgc tgactttgga ataatcttca tggatggtgg cggatacctt
    18901 aacatgtgtg gccatggtac aataggagct atgacagcag ctatagaaac aggtgtagtt
    18961 ccagcagtag agcctgtaac tcatgtagtt atggaagctc cagcaggaat aataagagga
    19021 gacgttacag ttgtagatgg aaaagctaaa gaagtatcat tcttaaacgt accagctttc
    19081 ttatataaag aaggtgttga agttgactta ccaggtgttg gaactgttaa atttgacata
    19141 tcatttggtg gaagcttctt tgctataata catgcaagtc aattaggttt aaaaatcgaa
    19201 cctcaaaatg ctggtaaatt aactgaacta gctatgaaac ttagagatat aataaatgag
    19261 aaaatagaaa tacaacatcc aactttagct catataaaaa ctgtagactt agttgaaata
    19321 tatgatgagc caactcatcc agaagctact tacaaaaatg tagttatatt tggtcaaggt
    19381 caagttgaca gatctccatg tggaactgga acaagtgcta aattagctac tttacatgct
    19441 aaaggtgaat taaaagtagg agaaaaattc gtatatgaaa gtatattagg aactttattc
    19501 aaaggtgaaa tagttgaaga aactaaagtt gcagatttca atgctgtagt acctaaaata
    19561 actggttctg cttatataac tggatttaat cattttgtaa tagatgaaga agacccactt
    19621 aaacatggat ttattcttaa ataagtaata aataaattag gaaattaaaa tagaatagta
    19681 gccacttgtc ggttgctatt ctatagcttt taggctataa gattcagaat atatgaaaaa
    19741 ataaaaaaat aggaggatgg attatggtag caatcttaaa ggcattatat gtagtattcg
    19801 cagcaatctt tgcagttgtg tttggtaaag acgtagctaa agcaaacaaa gaggggaaac
    19861 tagaagaaca aagtactcca aaagttgctg taacaggatt tataacagac ttctttgata
    19921 cattaggaat agggtcattt gctccaacaa tagcaatgtc aaaggcttta aagttaaata
    19981 taccagataa aaaaatgcct ggtacattaa acgtagcaca tactattcca gttgttacag
    20041 aagcattcat atttacatca atcataccag ttgatggtgt tactttagta tctatggttg
    20101 tggcagcagc agttggttca tatataggtg caggaataat agctaagatg gacgagagaa
    20161 aaatacaact tgttatgggt gtaactttag cattaacagc aatattaatg ttattaggtc
    20221 atccttggat taacgtatta ccaggtggag gaaatgaatt aggtcttact ggtgttaaat
    20281 tagtaatagg tgtaataggt aactttatac taggagcttt aatgacagct ggtataggtt
    20341 tatatgctcc aggtatggca atggtttatt tcttaggaat gtctgcaaaa gttgcattcc
    20401 caatagttat gggttcttgt gcattattaa tgccagttgc aagtatgaaa ttcataaaag
    20461 aagatgcata tactaaaaaa gcatcagtta taatagctat atgtggttta ataggagttt
    20521 tcgtagctgc atatatagta aaaactctac caatggatat attaaaagca ttagtaatag
    20581 tagttatagc ttatacttct gctacaatga taatggctgc aaataaaaac aaaaagactg
    20641 cttagttaaa gactacttag tgtttattaa atattacagc taataataaa gattataaac
    20701 tgctttaaaa tattgaatta atattttagg gcagtttttt atttactaaa ttaatataaa
    20761 gcaattaaca tgccaattat gtaatatata tagtgattta gtagatttaa tgtaaaaaac
    20821 gttgtatttt taaagaataa agcataattt aaagctaaat atttaaattt tataatcaat
    20881 aagttcaata aatgttcata tattaataca gattaataaa aatataacct ataataagtc
    20941 taatataact tataattaag aaaaataagt ttataatgac tcaatattca tctgaattat
    21001 ttgaataaat ttaattcgtt gaagttgctt atatagagtg aataaattta agttatatat
    21061 gacttattaa ctatataata ggatttttat aattaataca aaactgaaat aggtagttta
    21121 attagtatat gtggtacaaa aagataataa ttttataatt ataaaagtga aatattaaat
    21181 gaaatttata actaaataaa aacttttaaa cagcaatcaa agatttgtaa taaaatacat
    21241 cccttataaa tatatttatt atataagctg ataccagaaa tataagaatt aacaattgtg
    21301 gagggataaa atatgatagc tgttaaagat gagaataacg tagtaaactt aagaggggag
    21361 ttagacaata agctagaatt tagccatgag atatttggag aaaaatttta taatatgaaa
    21421 ataaaaataa acagactaag cgattctttt gatatacttc caatgactgt atcagaaaga
    21481 cttctccaag atattgacct tgataaacaa aatttagtca atgtcgtagg ccaattaaga
    21541 tcatataaca aaacattaaa taataaaaat agattagtgc ttacagtgtt tgtcagagaa
    21601 ataaaaagca tagacgagga aaataaagac cctaatagta tatttttaga tggatatgta
    21661 tgtaaggaac ctgtgtatag gaagacacct ttaggaaggg aaataaccga tttattagtt
    21721 gctataaaca gaccttataa taaatctgat tatataccat caatagtttg gggtagaaat
    21781 gctaaatttg caaagaatct aaaggttggt gatagaatac agctttgggg tagggttcaa
    21841 agtagagaat atgagaaaaa aatagatgaa aataatgtgg tcaaaaaaat ggcatatgaa
    21901 gtatccatat cgaaaataaa aaaattggat gaaaataaca atgaataaac ataaaaaata
    21961 gactgatttt tatgaataaa atcctaataa atttggtata atatcatatt aaaatgtata
    22021 aagattgttt aaaaaaatac taatgaaata tctcttgaat agttgctcgc catttattat
    22081 aatgtaatct gtacataatt tataattgag gaaatgaaga ggtgaagcat tgtgaaaagc
    22141 atattatgtc agataaataa acagccggtt aaagctaaat atacatctga tgaattagaa
    22201 tgttttttgg atacaattga gtataatgta ggtgataaaa tattgcttgt tggtcatata
    22261 ggaaacctag gcaagagatt gagaggtctt ggaacttctg taactatact ggagaatagt
    22321 aattatgaag atgtctgtta ttctctagtg tataatgaaa attgcaatgt agttaaaggt
    22381 aagttagagt ttctaccatt tgatgataat cagttcaaca agataataat agttgaccaa
    22441 tttaatgtta ttacagattg tgaaaaagct tcatctgaaa tacaaagagt gttaaagggc
    22501 aatggagagg ttatattaga ggatttaaat ttaaagaatt taaaagttaa gttaaactat
    22561 cttaaacata aattttgtgg ttctgttgca aagattcact atccacatga agtatttaat
    22621 atattttcaa gattaaactt tgatggtatg attaaagagt caaataaatt aaaatatatt
    22681 tatgtgggaa aaagaagata aatgagttaa aatataaata tggatataat ataaaagact
    22741 aggttaagcc tagtcttttg ttataaggag atgtttagat ggaaataaat tatgagttaa
    22801 taataattgt tatgctcttt gtaatcctga tgtcaatcca atttacttta aataagatat
    22861 acttgattct taaagaaata agacaaatac ttatgttaaa gaaaaataag gaatgatgat
    22921 tatgataggt ttagaatata taaaatctga tattcaaaat atagctgaag ctatagtatc
    22981 agtactcaat atagatgtaa ctatagtaaa taaggagtta tttaggattg caggtacagg
    23041 agtatatata gacaaaatag gtgagaaagt agataaatat acggctttta aaaaatcact
    23101 tacagagcag attacaatac taattgatga cccaaagagt agtgatatat gtagggaatg
    23161 ctataaaagt ggggcttgtg tagagtttgc agaggtgtgt tgtcccatta tatgtgatgg
    23221 agtctctcat ggtgttatag gccttatagc ctttacaaag gaacaagcac agattataga
    23281 aaataataaa agtggtctta taaacttttt aggcaaaatg gcagacttaa tatctaataa
    23341 attaaaagct caaattaaaa cttatgagct tgaattagaa aagaaaaaat tagagacact
    23401 gttaaacaat atggacaaag ctgttgtgtc tattgatgtg gatggaaaaa ttgataaata
    23461 taattcaaaa tttaaagagc ttttcaatat aaaggatagt ataactggca aaaacatatt
    23521 tgtcgaatta gattttataa agaaaccttc tataaataac tttaagaaag acaaatcctg
    23581 tagttttttt tataaaagat acggatatga ttcgaaggga atatacaaca tcaataaaat
    23641 agtattaaaa aatgagttaa agggatatgt tattgatttt atagataaaa gagatgctat
    23701 aaaaaactac aataagatga ataaagatta tagaataaaa ttggaaaaca taattggaga
    23761 aagtgtagct ataagaaatt ctaaaaagga agccctaata gcttctaaat caacatcaac
    23821 agtacttatt actggggaaa gtggaacggg taaggagcta tttgcaagag ccatacacaa
    23881 ccatagcaat agagtagata atccttttat agctgtaaac tgtgctgcta taccagacaa
    23941 tttattagaa agtgaattat ttggatatga agagggagct tttactggag ctaaaaaagg
    24001 tggaaagctt ggaatgtttg aagtagctca taagggaagt atatttttag atgaaatagg
    24061 agatatgagt ttgcatttac agtcaaagtt attgagagta ttacaagaaa aggaacttaa
    24121 taaaattgga agtaaatcaa atgtttcaat agatgtaaga attattgctg ctacaaataa
    24181 agatttagaa aaaatggttc aaaatggcac ttttagagaa gatttatact atagattaaa
    24241 tgttatacct ataaaattac caagtttaag agagagaaaa gaagatatcc cacttataat
    24301 caagcatatg gttaaagagt attcaaaaaa attagataaa aatgttattg acatagatag
    24361 cagggttgtt gatgctatga tggaatataa gtggccaggg aatataagag agttgcagaa
    24421 tattattgaa tatagtgtga atatgtcttc ttcttcaata attacaatga atgtaattcc
    24481 tcaaaaaatt aagaatacct ctgtagaaga gttagaagtt aaaagcgata ggataattcc
    24541 acttgatgaa ctagagaaga ttgagataat aaaagcttta aataagtaca aagattataa
    24601 aaaagataaa gaattaacag ctaaagcact tggaatatca agagctacat tatatagaaa
    24661 aattgaaaaa tacaatataa tttcatagta atataataaa aagctatata aaatttaaat
    24721 ttttctaaat tcataacttt aaagaaagct ttaaacttat actataagtg ttttgcagaa
    24781 tcttttttta tgaattaaaa aatggttttt ataagttaaa aaataatata tttgagccgt
    24841 ctcaaaatga tatagaatct cattttgaga cttttttttg tctttttgcg aaattgtcat
    24901 atgtttctcc attttaaaga atataaatac tctatacaaa taaaaaaaat acataaatag
    24961 aggcagtcat accaatggtt atagaatata agactaaaaa tataaaaaga tataaaaaat
    25021 atggcatgta ttttgctata aatatagata agaaattatt taattaatta tttgagtact
    25081 aaatctaaac ttaattggga ggtttatata taatgagaga tataagaaaa gaattagtgg
    25141 aaatgttaaa ggcagaagtt aaaccagcag tagggtgtac tgaaccagta gccttagctc
    25201 tagcatgtgc aaaggcaaag gaattattag gagaagaaat agttgaaaat cgtatgttag
    25261 ttagtcctag tatttataaa aatggtatgt gcgtaggtat accaggtact gaaagattag
    25321 gattaaaaat tgcagcagct ctaggtatag taggtggaca ttctgaaaat ggattaagtg
    25381 tgttagaaac tctaacaaaa gaggaagtta aaatagctga ggattatatg gataatactc
    25441 cactatcaat aacacctgct gatacaagag agaaagtatt tatagaagtt gtgttaaaag
    25501 gaaaaaatca tattgctaaa gtgagaataa gaactaaaca tgataatttt acgttcttag
    25561 aaaaagatgg agaagtatta ttagacaatg aaccaaaagt atcagcttca aatgatgcgg
    25621 cagaaaaagc tgaaagttta atggatacag taactataca agaattaata aaaaatgtag
    25681 aaggaattga ttttaaagat atagaatttt tactagatgg agtaaaaatg aatgaagaga
    25741 tggctgaata tggattaaaa caaaaaactg gtattggtgt aggatatgga attaaaaagt
    25801 ctatagaaga aggtctttta ggtaatgatg ttataaatta tgcaatgatg ttaactgctg
    25861 gagcttcaga tgcaagaatg gcaggggtaa aaatgcctgt tatgagttca aatggaagtg
    25921 gaaatcatgg attaacagca attcttccaa tcgttgctta taataaaaaa ttcccacaat
    25981 ctgatgaaag actagcaaaa gctttagcta tatctcatct agtaactggt tatattaaga
    26041 actatacagg aagattatct gctgtatgtg gatgtggtgt tgctgcttct actggagcaa
    26101 ctgctggtat ctcttggctt atgaatggta ctgaaaagca aatagaagga gctatagaaa
    26161 atatgatagc tgacttaagt ggaatgatat gtgacggtgc gaaagctggt tgtgcattaa
    26221 aattatcttc tgcagcatct gcagctatac aaagtgctat aatagctaag caagattgtt
    26281 ttgtaccacc actaaatggt atagttggtt caagtgttga gcaaagtata caaaacttag
    26341 gtagagtaag tgacaaaggt atgtctataa cagatgagat aatcttaaat gtaatgaatg
    26401 atatgaataa agttgactag tactttataa tatgttttgt attatgaggt ataacaaaaa
    26461 tagcataaat aaatttaatt agtaccctct aataaaatat gattatgtat aaatttatgt
    26521 ataatagaaa aaacttgtta atgcaagttt tttctttttt tgtctaaaat atatagtatg
    26581 taatttagtg tggatatatg aactacttaa aatataagat acattgttaa taattgttga
    26641 atgaacataa aaactcaata atattgacta atattgaata agagtattga aaaagtgagc
    26701 aatctcttat aaaattaaga aggattatta caattggagg tctttttatg gatatagaaa
    26761 caaaaaaatg ggaagtattg tattcagaag aaaaaattaa agataaatta agggaattag
    26821 gagcaattat agaaaaagat tataaagata aaaatcttat ggtagtttct ctattaaaag
    26881 gtagctttat attttgtgca gacttagtta gaaatataaa cttaccactt agagttaatt
    26941 ttatgactac atctagttat ggaaacaatg aagaaagtac tggaaaggtt aaagtagttt
    27001 cagatgtgac tactgatata gcaggatatg atgttttggt tgtagacgat attactgatt
    27061 ctgcacttac tatggatttt gtgttaaaac atctaaaagc taaaaatcct gctagtttaa
    27121 aatgctgtgt tcttttggat aaaccaagca gaagaaaagt cgatttagtg ccagattatt
    27181 gtggatttga aatagaagat aagtttgtgg ttggatatgg gtttgatttt ggtgattatt
    27241 atagaaatgt tccttatata ttcaatgtta ctgatgaaga tagataagca attatataat
    27301 tttgtggatg ccttaatatg atttttatta atcgtgttga ggttttttta tttaaaaagt
    27361 aattaaagta gtattaaatt aattttatgc aatttaatta tctatttttt atagcataat
    27421 aaaaaaattt taaataattg atgggaaatg agttttttaa ttgtattaat aattgtaaaa
    27481 gagtactaat aattgtaaaa gattttacaa aaaagtaatg aaatttaaga atcaaagcat
    27541 atatgttgat ataagcaaat agatacatta tcacataaga aattgtcgat tcatataatt
    27601 atatagagta atactcaata tttatagagt attactctaa ctttaaaaaa ggaggattaa
    27661 ttatatgagt gatatttcag gtccaagttt atatcaagat gtaggtccaa cagggccaac
    27721 aggtgctact ggtccaacag gaccgacggg gcctagaggt gtaactggag cgaccggagc
    27781 aaatggaata acaggaccaa caggaaatac aggagcaact ggagcgaatg gaataacggg
    27841 tccaacagga aatatgggag cgactggacc aaatggaaca acaggttcta caggaccaac
    27901 aggaaataca ggagcgactg gagcgaatgg aataacaggt ccgacaggga atacaggagc
    27961 aaccggagca aatggaataa caggaccaac aggaaacaaa ggagcaaccg gagcaaatgg
    28021 aataacaggt tctacaggac caacaggaaa tacaggagcg actggagcaa atggaataac
    28081 aggtccaaca gggaatacag gagcaacagg agcaacagga ccaaccggac taactggagc
    28141 aacaggagca accggagcaa atggaataac aggaccaaca ggaaatacag gagcaaccgg
    28201 agcaaatgga gtaacaggtg ctacaggccc aacaggaaat acaggagcaa caggtccaac
    28261 aggaagtata ggagcgactg gagcaacagg aacaactggg gcaacaggtc caataggagc
    28321 aacaggagca acaggagcag atggagaggt aggtccaaca ggagcagtag gagcaacagg
    28381 tccagatggt ttggtaggtc caacaggccc aacaggccca accggagcaa ccggagcaaa
    28441 tggtttggta ggcccaacag gcccaaccgg agcaaccgga gcaaatggtt tggtaggtcc
    28501 aacaggagcg acaggagcaa caggagtagc tggggcaata ggtccaacgg gagcagtagg
    28561 agcaacaggc ccaacgggag cagatggagc agtaggtcca acaggagcga caggagcaac
    28621 aggggcaaat ggagcaacag gcccaacggg agcagtagga gcaacgggag cgaatggagt
    28681 agcaggtcca ataggtccaa caggtccaac gggagaaaat ggagtagcag gagcaacagg
    28741 agcgacagga gcaacagggg caaatggagc aacaggccca acaggagcag taggagcaac
    28801 gggagcaaat ggagtagcag gagcgatagg accaacaggc ccaaccggag caaatggagc
    28861 gacaggagca acaggggcga caggagcaac cggagcaaat ggagcaacag gtccaaccgg
    28921 agcgacagga gcaacaggag tgttagcagc aaacaatgca caatttacag tgtcctcttc
    28981 aagtttagtg aataatacat tagtgacatt taattcatca tttataaatg gaactaatat
    29041 aacttttcca acaagtagta ctataaatct tgcagttgga gggatataca atgtatcttt
    29101 cggtatacgt gccacacttt cacttgcagg atttatgtca attactacta actttaatgg
    29161 agtaactcaa aataacttta ttgcaaaagc agtaaataca cttacttcat cagatgtaag
    29221 tgtaagttta agctttttag ttgatgctag agcagcagct gttactttaa gttttacatt
    29281 tggttcaggc acgacaggta cttctgcagc tggatatgta tcagtttata gaatacaata
    29341 gtttatatga tgaaaagatt ataaaataat aaacaatatg atttcatata ccaataaata
    29401 gaataatatt taataggttt atatgatgaa aagactataa aataataaac aatatgattt
    29461 catataccaa taaataggat aatatttaat aggtttatat gatgaaaaga ctataaaata
    29521 ataaacaata tgatttcata taccaataaa ttaggataat atttaatagg tttatatgat
    29581 gaaaagatta taaaataata aacaatatga ttctatatac caataaatag gataatattt
    29641 aataggttta tatgatgaaa agactataaa ataataaaca atatgattcc atataccaat
    29701 aaataggata atatttaata ggtttatatg atgaaaagac tataaaataa taaacaatat
    29761 gatttcatat accaataaat aagataatat ttaagaaaaa tgattatatt taaaaggtaa
    29821 gcataaaatt tataaacttt tagaatagaa atctcaatca attagatatt caaattgatg
    29881 aatatgaata cctaaataga ttgagattta ttatgtacaa aacttatcct aatatatctg
    29941 acattttata aagcccagat tgcttaccaa ttaaaaattt agctgcaact atagagccgt
    30001 ttgcaaatac ttcatcagat tgagaatgat ggcttaatgt aagaacttca ttatcacctg
    30061 caaatacaac atcatggtcg ctgattatat tgccacctct tatagagtgt attccaattt
    30121 catttggttt tcttttacaa tctctaccat atctaccatg tgtattttca ctttcaggca
    30181 agacttctct aattgcatta gctatcatag tagcagttcc actaggggcg tcttcttttc
    30241 tattgtgatg tttttctatg atttcaatat caaaaccttc taacatcttt gaagcatcct
    30301 taactaattt tattaatata tttactccaa gagatgtatt tgatgataat aatataggaa
    30361 tgttttttga tgcttcttct attttattta agtcattttc gtcatatcca gtagtagcta
    30421 atactacagg tgtattattt tttagagcat atgacaatat tggctctaaa gtatcatgat
    30481 gtgaaaagtc tataattaca tcagtttttt cagttacatc agtcattgtt ttgtaaagct
    30541 ttgtatcact atgatgacct ttttcatcag cagacctagc tacaccacaa accaactcta
    30601 attcattatc tttttttaca cattctgcta aaactcttcc cattgttcca ttatagccac
    30661 taattataac tttcaacata aattcctcct atgaaattaa taaaaatttg ttatatttct
    30721 ataagtttat tttatcacta atataattta tataaaataa tattagtgat aataagctta
    30781 caatttatct tacattaaaa aatttaagtg gatttttcaa aatatagata aatttctttt
    30841 tcattgttta aatgataaaa atatatttga cttcatacca aaaagagtgc cccatgaact
    30901 aaaaagttaa ttttgaaata ccatattcat atatcatatg atttatttgg aaattaatat
    30961 taatacatta aaataactaa atacatgtat gcataaagat gtataaaaaa tatataaaca
    31021 tatagtatta attttaaatt caacaattta attgttaaaa gtgttattat taaattagaa
    31081 atatcttaac ttaatatact gagatgggag tgatagatat gttaaaagaa gaaaaaactt
    31141 atcaagagaa agacgcaaaa gtattcaatg gagtggcagc tttgataatg attttattat
    31201 ttttagtagt ttcaataggt atcatggttt ttgggatagt aaaacttgat agtggattta
    31261 ctggaatagg ttcacttatg ttaattttgg gacttgtatt tatagtagta gattttatcc
    31321 tattttttgg gctaaaaatg attaatccta aagaagctat agttctagtt ttgtttggta
    31381 actattatgg aactataaaa aaagaagggt actattgggt gaatccattt tgttcagcaa
    31441 taaacccagc agtatcaaga attaatgtat cgaaatcttc atctgatgaa aaggtagata
    31501 tatcaactaa ttcaaggggc aaaaaagtat ctttaaaaac aatgactctt aataatgaaa
    31561 agcagaaagt caatgattta ttgggaaatc caattataat aggagtggtt gtaatttgga
    31621 aggttataga tgcaacaaag gcagtattta atgtggataa ttataataca ttcttatcta
    31681 ttcagtgtga ttctactatt cgcaatgttt caagacttta tccatatgat gtatctgaag
    31741 atggagatga aaaatctctt cgtggtagta gtcaggaaat agcagataga ttaaaagatg
    31801 aattacaatc aagggttgac attgcaggaa ttgaagtgtg tgaagtgaga attactcatc
    31861 tttcttatgc accagagatt gcagcagcaa tgttacaacg tcaacaagca gaggctataa
    31921 tagcagcacg taaaaagatt gttgaggggg ctgtaagtat ggtagagatg gcattgacta
    31981 atttaagtga aaataatgtt gtgactttgg acgaagaacg taaagcagct atggtaagta
    32041 atctgttagt tgtgctttgt ggcaataaag atgcacaacc agtggtaaac agcagttcta
    32101 tttattaaat tataaaaagt ttaatagttt attttattga tatatagaaa atttagtagt
    32161 tcattttatt aaattatata aagattaatg gtttgcttta ttgaattata aaaagtttaa
    32221 taatttattc tattgatata tagaaaattt agtagttcat tttattaaat tatataaaga
    32281 ttaatggttt tcactttatt aaattataaa aagtttaata gtttattaca ttgatatatt
    32341 gaaaaattga aggaaataaa aatacttgtc agcaagaaat aaaaatgata taagaatgaa
    32401 taagagaatt gtaaaaaaat aaaatgatta taaataaaag ataaaaatat tgtaaaaaag
    32461 agggtgcctc tggtgaatta aattcacaca gagacacctt tttaattaat tttttaaatt
    32521 agtatattat tgtttaatta atagcatagt actatttaat taatctaaat ttctaaggtc
    32581 ttccataagc ttagttttgt cagccgtttt ttcatctttc atttttataa ctttagcagg
    32641 agaaccagca acaacggcac cagcttctac atcagtagtt acaacagcac cagctgcaac
    32701 aacagcacct ttaccgattc taacaccttc aagaataact gcattagctc ctattaaaac
    32761 atcatcttca actataacag gagtagcaga aggtggctct aaaacaccag caacaacagc
    32821 accagcacct aagtgaacat ttttaccaag agttcctcta gctccaataa cagcattcat
    32881 atctaccata gaaccttcac caataacagc tcctatattt ataacagctc ccatcattac
    32941 aacagcattt ttttctatag taaccatgtc tcttattatt gcacctggct caattcttgc
    33001 atgttcactt aaagtatttt ttagaggaat agcagaattt cttctatcat attctacatg
    33061 agtatctgtt atatcatttc catgtttatc taatacttcc attatatcct catagtcacc
    33121 tacaaatata taagaaccat tacttccaaa taccttaaat ttatcagttg attggaagtg
    33181 taaagagtta gtatttacat ataccttaac aggtgttttc tttttaactt cttttatata
    33241 ccttgctatt tcataagcat cgtttaaatt taccatttgt taattctcct tgtatttcat
    33301 tatatttctt ttattatata cttatttgtc aattttagta tatacttact tcttattatt
    33361 tatattttat tttagttagc ttttattgta tagacatagt attaaataaa tttattatat
    33421 atgtttgatt tgtattgcat attattatat atatattgta tatgtttaat ttatattgtg
    33481 tattattata tttatactat tatcccaaca tgctgtccat attataaaat cctggttttt
    33541 gcttaactaa gtatttggca gcagctatag aaccacttgc aaagatacca cgagattgag
    33601 cttgatggct tattgtaaga acctcattgt ctccagcaaa tagagcatca tgttcgccaa
    33661 caatatttcc acctcttata gcatgaatac caacttcatt tggttgtctt tttgcactac
    33721 gaccatatct accataatta ttttcaacgt ttggcaaaac ctcttttata gcgttagcaa
    33781 tcataacagc tgtgccactt ggggcatcta cttttctgtt gtgatgtttt tctattattt
    33841 ctatatcaaa tccttctagt aatttagctg catctttaac caattttact aatacattta
    33901 caccaagaga catattatat gattggaata caggaataac tttagccgct tcacgtattt
    33961 tgttcatttc gtcatcatta tatccagtag tagcaattac taaaggtgtt ttagtcttta
    34021 aaacgtaaga taagatatta tctaaattag agtgatgaga aaaatctata actacatcag
    34081 cttcctcaac tatagtactc atatcttcat aaattttaag gtcgccttca tagctatcca
    34141 gacctctagc tgcaacacaa accaattcta attcattatc ttctcttata gtatcggcta
    34201 aaatcttacc catttttcca tcataaccag ttgctataac ttttaacata atttcctcct
    34261 atgattaagg gctaactttt gaccaaactt actttttacc atgcattaat ctttactatg
    34321 cattaacttt tagtccaaag ttaactaatt ctttctttaa tacttctaaa tttgctgggt
    34381 ccatttctgc tagaggtaat cttaaatctc caacattgaa tcccaataaa ttcattgcag
    34441 tcttaactgg tataggattt acttctataa ataaagctgc gattagagca tccataccaa
    34501 gttgtaattt tctagaacct tcaatatcgc cttcaaagaa tttagctacc aaatcgtgag
    34561 tttcttgagg gcaaacatta gcaagtactg atataactcc ttgacctcct aaagataata
    34621 aaggaagtat agtatcatca ttaccagagt agatagcaaa gtcatctgga actaaactag
    34681 ctatttctgc aacctgtcct aggtctccac tagcttcttt tacagcaact atgttatcaa
    34741 ttttagcaag ttcaactata aggctaggac ttatatttac acaagttctt ccagggacat
    34801 tgtaaagtat aattggaagt ttaacactgt tagctattgt ttcaaaatga cgcttaagtc
    34861 cagctttatt agctttattg taataaggtg ttattattaa tagagcatct actcctaatt
    34921 tttcagcttc ttgacttaag ttaactgagt gcatagtatc attagaacca cttccagcta
    34981 taacaggaac tcttttatta actttttcaa caacatactt aatagtagca agttgttctt
    35041 catcagtcat agtattagct tcaccagtag taccacaaga tactatagca tcagtaccat
    35101 tagctatttg gtattcaact aattcaccta atttgtcaaa atcaactttg ttatcctttg
    35161 taaatggtgt aactagagca acagcagaac ctttaaatat catataaaat caatcccctc
    35221 ttttttattt ttgtgtttta tattacagtt acttctaatg cttgtttttg agttactatt
    35281 aatattttac aaaattatat atttatacaa gtttgtatat tatactaaat cgtatgataa
    35341 agccatttca gctatttgaa ttgcgttagt tgcagcacct tttcttatat tatcagcaac
    35401 aacccaaaga tttacaccat tatcaacact aaaatctctt cttattcttc caacaaatac
    35461 ttcatctcta tcatcaaatt ctacagcagt tggatattcg tttttagcag gattatcaac
    35521 tactacaaca cctggagctt cttcaagtgt ttttactaaa tcatctaact caaatggatt
    35581 gttaaactca acatttatag attcactatg accatttttt actggaactc ttacagtagt
    35641 tgcagttatt tttaaatcgt agttatcaag tattttcata gtttcattta ccattttcat
    35701 ttcttcttta gtgtatccat tttcagtgaa tgaatctata tgaggaagac agttgtaaga
    35761 gatttgatga gggtagaatc cagtaggatt accgttcatt cctttctcta aatcttcaac
    35821 acctttaaca ccagaaccag atacagcttg gtaagtagag taaacaactc tcttaatttt
    35881 gtatttatca tcaagagctt taaggggaac catagcttgt atagtagagc agttaggatt
    35941 agctattata cccttatgat tttttatggc ttctggatta acctcaggaa ctactaaagg
    36001 cacatctgga ttcattctcc attgactaga gttatctact actaagattc catttgaagc
    36061 agctattgga gcatactttt cactaatact tccaccagca gagaataatg ctatttgtat
    36121 gcctctatca gtaaatgaag tttcctttaa ttcttctact atatattctt taccacaaaa
    36181 cattacttta gaaccagctg atttagaaga agagaataaa aatagatttt ctattggaaa
    36241 atttctttct tctaatactt ttaaaaatgt tctacctacc attccagtag caccaattat
    36301 tgctacattt acttttttca tataaattca ccccaatttt aaattattaa aacattattt
    36361 tattttagtt atacaagtaa gatatgttaa tatttttata tatccaaaaa ctttaatatt
    36421 atattaacaa ctttaattat atacaaaaaa cgtacaaagg tatactttgt acgtctaatt
    36481 tatcactcaa agtaaaccct gtagctcatc acctacaaaa ataagtgaca gtattacaca
    36541 tgttatgtgc aatcccagaa aatattattg caagaaatat tttcttcggc tgtaaatcct
    36601 ttttccaatc attaacacct gccagctact attggatact cttagttaca gctacctcta
    36661 ccctaggtta ataggatatt aaaatttgat tattctaata ataatacata tttaagaaaa
    36721 aatcaactta tttatataaa tattgctaaa atttaaatcc aaaatttgtc aattcttgtt
    36781 ttaacacatt aagattagtt tcttccattt cagctagagg aagtcttaaa tcaccaacat
    36841 taaaacctaa aatgttcatg gcagttttaa caggaactgg atttacttct ataaataaag
    36901 cagcaattaa agcatccata tcaagttgta atttttttga gccttcaata tcaccttcaa
    36961 agaatttagt tactaaatca tgtgtttctt taggacatat gttagcaagt acagagataa
    37021 taccacttcc acctaatgat aatagtggta gtatagtatc atcattaccg gagtatatag
    37081 caaagtcttt aggtactaat ttagctattt ctgcaacttg agctaagtct ccacttgctt
    37141 ccttcacagc aactatatta tctattttag caagttcagc cacaacagat ggctttatat
    37201 tcactttagt tcttcctggt acgttgtata aaataattgg caacttaacg ctatttgcta
    37261 ttgtttcaaa gtgaagttta agacctttct cattagtctt attatagtaa ggagttatta
    37321 ttaaaagacc atccacccct aatttttcag cttcttgact catatgaaca gacaacatag
    37381 tattgtttga accagtacct gctataacag gtattctctt attaactttt tcaactgtat
    37441 attttattac agcaaaaatt tcagattcac tcatagtagt cgcctcacct gttgttccac
    37501 aaacaactag tgcatctgtg ccattctcaa tgtgatattc gattaattca cctaattttt
    37561 caaagttgac attattatcc tctgtaaatg gggtaactaa agcaacagca gaacctttaa
    37621 ataacatata atcaaatctc cttttttatc taaatttaat tttttaagta caataataat
    37681 tatagtacaa attaattaat tttaataggt atagttaata tcttaactgt ttaaataaga
    37741 tatttatatt atattaatat tagaaaaatt ttaaataaat gttttattct caaaatatgc
    37801 atattaattt aataaaatat accaaaatgg aaataaaaaa taaattttat gagaatttaa
    37861 taactaataa ttaatatatt taggttataa taaatatgtg cgtaaaatct aacaataagc
    37921 aataataccg taaatttagg aggaataata tggatacttt aaaggaactt tttaaaatag
    37981 gaagtggacc ttcaagttct catactatgg gaccacaaag agcagcagag agatttaaga
    38041 atgaaaaccc agatgcagaa agttttagag caatattata tggtagtttg gcagctacag
    38101 ggaaaggtca cttaactgac tatataattg aaaaaactat agcacctaaa aaagtcgaaa
    38161 ttgtttggga agaagatatt ataaaagatt ttcacccaaa tgggatgaag tttgaagctt
    38221 tagataaaga taaaaatgta acagctgaat ggactgtata ttctgtaggt ggaggaacta
    38281 tagcagaaga aggtcaaaga aacagtaaaa gcaatagtat atatcactta gatactatgg
    38341 atgagatagt aaaatggtgc aaagaaaata ataagacttt ggtagatttt gtgttggaat
    38401 gtgaaccaaa agacataaaa gattatataa aaactataaa agatgctatg agaaaatcta
    38461 ttgatgatgg actaagtaca gatgaaataa taccaggcaa gttattatta aaaagaagag
    38521 caagtacatt ttataatgca tataaaaaag ataagagctt ctcaacactt gtatatgcat
    38581 atgcactagc agcttcagag caaaacgctt ctggtaatat aatagttaca gcaccaactt
    38641 gtggttctgc tggagtaata ccaggaatat tctttgcaat gcaggatttc tataattatg
    38701 atgacgaaaa aataattgaa gcactacttg tagctggaat aataggtaat ataataaaaa
    38761 ctaacgcttc aatatctgga gcagaggtag ggtgtcaagg tgaagttgga gccgcttgtt
    38821 ctatggcagc agcagcagta gcatacttaa aaggtggaac tatagaccat attgaatatg
    38881 cggcagaaat agcacttgaa catcatttag gaatgacttg tgaccctgta tatggatatg
    38941 ttcaaattcc atgtatagaa agaaatgcaa tggcagctca aagagcttat gatgcagcta
    39001 attatgcatt attaactgat ggctcacatt ctgtctcctt agaccaagtt gttgaaacaa
    39061 tgaaagaaac tggtatagat atgatggata agtacaaaga aacagctaag ggtggacttg
    39121 ctaaacattt cttctcttgc taaaaataaa attttccaat aataattcct ctgttttgat
    39181 ttatgaaaca ggggaattat tattaatggt gaattattta ataatgcaaa atatttttat
    39241 attcaattta gctttttatt tttaattagt ataacatgct atataatatt ttaattataa
    39301 agaaatatag aaatattttt taataaatta gaaattggag gagattacat gagagaggca
    39361 tcatatttac aagaacaact ttataaacat aggaagtcat taaatcctat tgcagagatt
    39421 gggagaaaag agtataagac ttcaaaatat ataagagatt atcttgataa attaggaatt
    39481 gagtatgaag tttatttaga aacaggtata gtaggtgtaa tagaaggtaa aagacctaaa
    39541 aaagatattg cttttagagc tgacatagat ggtcttcata gtgaagaagg ggtaaaacat
    39601 ttatgtggtc atgatggaca tgctactatc ttacttggat taattgagta tttaaatgat
    39661 aataaagaag acttaaatga taatatagta tttatattcc aaccggcaga agaaggtcct
    39721 gggggagcaa aagctttaat agatgaagga gtgcttagaa aatacaatat tgatgaaata
    39781 tatgggcttc atatatatcc agagttacca gaaggttatg taggtacaag agagggctat
    39841 tttatggctc aaataggaga tattgacata gaaatagtgt ctaaaagtgg acatggggct
    39901 atgccacaaa atggtattga tggtatagtg atagcttcaa actttgtaag ctcacttcaa
    39961 actatagtgt ctagaaatat aagcccaata gacaatgcag tgttgactat tggcagaatt
    40021 gaagggggag ctagaagaaa tataatagct gaaaatataa agatggaagg tactatgaga
    40081 tgtttcagcc cagaagtgta tgagaaaatg aagcttagag ttagagaact tgctagaggg
    40141 tttgaattgg cttataactg taaggttaat ataaatatta tagatgatta tatcgctgtt
    40201 aaaaatgaca aggatttgta tcaagagttt gtagaggcta ttggaagtga tactgttgta
    40261 gagttagagc cacttatgat atcagaagat ttttcttatt atcaaaaaga agttccagga
    40321 ttatttttta tgttgggttc aagaaacgaa gaaaaaggat ttgtaaatgg tcttcataat
    40381 ataaacttta attttgatga aaagatatgt gtaaatgcat tgaatgttta ttcaaaatta
    40441 ttaaaataca aagaagcaat agattaggaa gataagttat aaagattaaa aagcataaat
    40501 acctaaattt aaaaaaagtg tattaataaa taccccttac atatttgtat aaatttattt
    40561 taattgacaa aaatacaaat aagaaggggt gatttttttg aaagaaaaag tacaaagaga
    40621 aaagaagata actaatggca agaatactaa aatattgaca aatgatgata taatgaaata
    40681 tgagatagcc tcagaactag gtcttatgga taaagttggt gaacgtggat gggcaggact
    40741 tacagctaag gaagcaggaa gaattggagg tatgcttaca tcacgcaaaa agaggcttaa
    40801 aaagattgtg gaggagcaga ataaaaagga tgagtcaata tagtgatttt gcgtttgtat
    40861 atgatgaact tatgaatgaa gttgattata atggttgggt taaatatata gaagatataa
    40921 tagatagtga aggtgtaaaa gtaaaaaata tattggaatt ggcatgtggc acgggtaatc
    40981 ttacaattcc attgactaaa aaaaattacg atatagctgg tatagatata tctgatgaga
    41041 tgcttagtgt agcaagagaa aagtcagaaa aagaaggagt agaattagtt ttacttcaac
    41101 aagatatatc agagcttgat tttgaaatat ctgatttgga ctgtgtactt tgtgcttgtg
    41161 atggattcaa ttatataact tatgatgatg atttggaaaa tgtattttca aagacatatg
    41221 aattattaaa gaaagatgga atattcatat ttgatataag ttctttttat aaattagcca
    41281 acatattggg aaataacatg tatggagaaa atagagaaga tatagcgtat atgtggcaaa
    41341 attactttga tgaagaagag aatctaattg aaatggaact tacattcttt attaaagatg
    41401 aatgtggaaa atttgaaaga tttgaagaga tacaccaaca aagagcatat actgaagatg
    41461 agatagttga tatgcttgaa aaatctggat ttaaggatat aaaatattat ggggatttta
    41521 catttgacaa acctagtgaa gaaagtcaaa gaatcttctt tgtatgtaaa aaataagata
    41581 tttttgacta tataagataa ttattaaaaa taaccatgat ataaaaatat agaaaatgca
    41641 gttttataga ttaaaagtaa agtgatgata ctttatgact tttatttttt aataattata
    41701 aagttctata gataaaaatt aaaatataat aactattgct tagtaaaatt aaaataaaat
    41761 gggttttgcc caaggttaag atagataagt atttatagat taaaattaag gtagtaatat
    41821 aataaaaata agacaattaa cgggacatgg aggaatatta atgagagatt atgtactaag
    41881 agcgacatca ggaaatggtc aagttagagc atttgtcgca actactagaa atacagtaga
    41941 agaagctaga agattacatg aaactacaaa agtagcaaca gcagcacttg gaagaacatt
    42001 aacagccaca tctataatgg gattaatgat gaaaaatgat agtgataaat tgactgtaat
    42061 aataaaaggt ggaggtccaa ttggaactat tatcgccaca tcagactcta agggaatggt
    42121 aaaaggatat gtaggaaatc cacaagtaga ggttgaagac tatccaaatg gaaagttaaa
    42181 tgtagcagca gcagtaggta cagaaggtgt tgtaaaagtt ataaaagact taggtcttag
    42241 agaaccatat aatgggactt atccattggt aagtggagaa attgcagaag actttactta
    42301 ttattttgct gtatcagagc agactccatc agtagtagct ttaggtgttc ttacaaaaga
    42361 agatgaagta gagtttgctg gaggatttat agttcaatta atgccagatg cagaagaaga
    42421 gactattgct aagttagagg aaaatgtagc taaattacct tctataacta acatgttaaa
    42481 agaaggaaaa tcaccagagg atatattaaa tattgttttg gatggtttgg aacctaaaat
    42541 acttgatacc tgtgaagttg ggtttatgtg tgaatgttca aaagaaagag taaaaactgc
    42601 tttggttgca attggtaaaa aatcattggc tcaaataata gaagaagata aaaaagcaga
    42661 agttggttgc cagttctgta ataagaaata tatgtatagt gaagaagagt tgcttgaaat
    42721 tttaaaggaa atgtagacta taaaaatagt tgtattgata taagtaatgt tatagtgata
    42781 tagtaaagtc gtattgatat agtaattaaa gtataattta ttaataatag acatatttat
    42841 attaaaaaat aagtttaata aatttttaag cttaataaat tttaagaatt aagaagcttt
    42901 ctggctgtaa tgtttgcaat gttgcagtta gaaggctttt ttattttatt atagttcgtg
    42961 tcaaagattg tatgaaacaa taaaatattg taaaataatt tgtatattta gacctgattg
    43021 gcacaaaggt ttataaggta tttgagttga ttatgcgaaa ttctatgaag tagtaaccct
    43081 gtgactttaa tcatgggagg ttcagaagaa ggagaaaatt ttaaatatgg aaagtaaaac
    43141 gaagtacata ggaataagta tattaatatt attgatgata tttttaattg caaaaaattt
    43201 taacggaaaa cttgaaatac ctattcctga gagtaatagt agaaaattta aatcagcagc
    43261 agtgttttat cctcaacatc aagatgatga ggttttatgg ggaggaagtg ccattgtaga
    43321 tgctataaaa cagtgtggtg ttgataatgt atatgtggta ttagtatcag atggttctgg
    43381 agttaacgtt ttcaaagcta acacaaaatt tagaaattta actaggaagc aaaaggaaga
    43441 acttagaaat aacgagttta aatcagcttt gagagagctt ggagtgaaac ctcaaaatgt
    43501 gattatactt gcagatatag ataaaaagga aggcacacat tatgaattaa tggaaaagac
    43561 tatattggat tttgaacata agtttaaagg caatgtgacc catatagccc atcactataa
    43621 gtatgatgac catataatgc atagaaagaa tggggaagtt ttaaagaaac ttaaacgtga
    43681 aggtaagata aaagatgact tgtattttgt taagccaaaa tatgtaaagt atataccaag
    43741 aaatgaaaga gctatctata aagcaatgga ttttgatgac tacaataagg ttaagagtgc
    43801 ttgttatgaa tataaggttg taaattcaaa gcgtaaaatg tatggtatag gatatatttc
    43861 ggcacatagt tattttgaac atcttctaaa tgaccctgaa cttacgtctg tattaagtga
    43921 agattaattt cttataagta gtaagttatg tttgataagt tgattggaat tttctatagt
    43981 tttgaaggta ttggaaattt agttttattt aaagatttaa agatagaggt ttttagtgtt
    44041 taaaatttta aagttaagtt gaataattgt atagaattat ttgtgaaaga agaaacagtg
    44101 taaaacatag tatcttattt atggttttaa aatcataggc aagatacttt ttttatgtaa
    44161 ctaataccat tttgagatta ttaacttaag aattttatat aatttaacac tcatatataa
    44221 tgatgatatt taggtagaat agatgcaatt acgcttaaat aaaataactg tattttttat
    44281 aaaaatcaaa ccctacacta tactaccaga taaaatctac atgctataat ttgacctgtg
    44341 tttataaaaa aaggagtaat ctaataaaat agtatttcac ttatgtataa ctaacaacta
    44401 ttattacgat tttctctaaa taatttaaag aaaaataatg caggaggtaa aatatgaaca
    44461 atgaatttat aattttaaaa ggttgcaaag agaacaattt gaacaatatt tccttaaaaa
    44521 taccaaaaag aaaaataacc atattcacag gggtgtcggg ttctggaaag tcatctattg
    44581 tatttgagac aattgcaaaa gaatcacaac gccaactaaa tgaaaggttt agtacatttg
    44641 taagaagttt tttacctaag tatggagagg ttaaagctga ttgtatagaa aatttgtcaa
    44701 caccaattat catagaccaa agtagacttg gtggaaattc aaggtcaacc ctaggtacta
    44761 ttacagatgt aaattccttt ttaagagctt tatattcaag gtttggtagt gagtatatag
    44821 gtaatgctaa tatgttttct tttaataata ttgatggtat gtgcccacac tgtcatgggc
    44881 ttggaaaaaa gctagttcct aatatgaacc aaattcttga tatgaataaa tcattaaatg
    44941 aaggtgctat attgctttca gggtttggag tgggttcttg gcattggaat atattcgctg
    45001 aatcaggatt ttttgatatt aataagagga tatgtgatta ttcagaagta gaattggaaa
    45061 agtttttgca tggtaaagca gaaaagatta agatagaaaa tgctggtcaa actaacatga
    45121 catatgaggg attaatggtt aaatttaatc gcctatactt aagtagagag ggagaaatat
    45181 ctgaagctac taagaaaaaa ttgagtaaac ttcttataga agatagatgt cctatatgtg
    45241 atggaagaag acttaatgaa agagtatata ggtctttaat taatggttat aatatagcag
    45301 atttaactag tatgcaaatt gatgaattgg ctgaagttat taaaagtata gatgagccag
    45361 aagcacaacc tttaattaaa ggaataatag aaaagttaaa cagtatcata gatataggtc
    45421 ttggatattt aacactggat agagagacat cttctctatc aggaggggaa tctcagcgta
    45481 taaagatggt taagtattta aatagtaatc ttgttgattt aatgtatatt tttgatgaac
    45541 caagtgtagg acttcatcca aaagatgtgt ataaattaaa taatctttta aaaaaattgc
    45601 gtgataaagg gaatacaatt atagttgttg aacatgaccc agatgttatt aagattgcag
    45661 accatattgt agatgtagga ccaaaggcag gtaagtatgg tggagaagtt gtgtatgaag
    45721 gcagttatga aaatctactt acaagtggta ctttgacagg gaatgcttta agtaaatttc
    45781 ttcctattaa agaaagtgta agagaacata gtggatattt agaagttaaa aattgtaata
    45841 aaaacaattt aaaaaatgta tcgattaaga tacctaaagg tgtattgact ctagttactg
    45901 gagtagcagg agcaggtaaa agtacgttga ttaaggatga atttttaaag caaaatccta
    45961 gtgctgtact tatagaccaa agccctgttt cagcaaattc aaggtcgagt ttggcaacat
    46021 atagtggaat tatgaacaac ataagaagca tattttcaaa aacaaatggc gtaaatgctt
    46081 ctttattcag ttcaaattca gaaggagctt gtgataactg taagggttct ggaatagtag
    46141 aaatgaatct tgcttttatg gagagtataa agagtacctg caatgtttgt gaaggtaaaa
    46201 aatttaaaaa agaagtttta gaatataggt ttcaagggaa gaatataatt gaagttcttg
    46261 aaatgtctgt tttagaggca attgagtttt ttaatttaaa acagattaaa acgaaattac
    46321 aatgtattga aaaaatgggt attggatacc ttactttagg acaaactctt gatactttgt
    46381 ctggaggaga gtgtcaaaga ttaaagcttg ctagtgaatt aaataaggaa agttcagtgt
    46441 atatattaga tgaaccaaca acaggtcttc atatggctga tattgagaat tttataagta
    46501 taattgaaga gattgtagat aatggaaata cggtcataat aattgaacac aatatagatg
    46561 taattaaaag agctgattgg attattgagc tgggacctga aggaggaaca aaaggcggga
    46621 gagtaatatt tgaaggtatt ccaaaacaat tatgtaacaa taaaatatca ttaacagcaa
    46681 aatatatagt ataataaata aaatataata aaattataat gatattttaa taaatataat
    46741 gggtatataa aatgataatt aaacttaaca gtaataaata atgtgagaaa tattcaaatt
    46801 taattaaata aagtgtcaga aatttgttta aattttagta ttttaacggt tctgaccttt
    46861 tttcttattt ttatggagaa tatcgaggat ggagtctcta atagttactt ttttgtgtct
    46921 acttgcaagt ttgattaaaa aatggctata cacacttgtg acttcagtca tgggaggttc
    46981 agattataat cttaatatat aatttgtgaa ataatttgat gacgatttgc tgatgtataa
    47041 acaataaaga ttaactacat taaaatttaa atcttgttaa gtaaaactta taaaaggaga
    47101 gagatggatg ataaatgata ttctaacctt attatctgaa agaagtgatt tttttataaa
    47161 gctatccata gaacatttga tgatttctat tatttctata ataattgcga ttgttttggg
    47221 tggtataatt ggtattattg taagtgaatt tcataattct tctaaaccta tattgggaac
    47281 tatcaatttt ctatacacca ttccatctat ttctatgcta ggatttctga taccattttc
    47341 cggaattgga aatactactg ctgttattgc acttaccatt tatgcattat tgccaatggt
    47401 tagaaataca tatactggaa ttactaatgt ggataaaaat attcttgaag cagctagggg
    47461 aatgggaagt acagattttc agatattgta taaaataaaa cttccgttag ccctacctgt
    47521 tattatgtct ggtattagaa atatggtgat tatgacaatt gcattagctg gtatcgcttc
    47581 atttataggt gctggaggtc taggtgtagc catatataga ggaatcacga caaataacac
    47641 aataatgaca attactggaa gtatacttat tgctctatta gctttaatat ttgattttat
    47701 tttagggctg gtagaaaaat tttttcaacg aaaaagaaaa aagaaaaata ggaaaaaagt
    47761 ttttgccatt ctaggaagcc ttattcttat agcaactatc tttacgtctt atttatttaa
    47821 tccttcaaag actcaaacta ttcatattgc tactaaacct atgactgaac aatatattct
    47881 tggtgagatg ttggatattt taattgaaaa aaacacaggt ctaaacgtgg aaattacaca
    47941 aggtgtagga gggggaacat ctaatatcca gccagctatg gagaatggag aatttgacat
    48001 ttatccagag tatacaggta caggctggaa tatggtactt aagaaaaatg aattatatac
    48061 tgaaagtatg tttagtaaac tacagtcgga atacaaaact aaacttaaaa tgcaatggac
    48121 tggtatgtat ggtttttcta atagctatgc attagcagtt agaaaagaag ttgcagataa
    48181 atacaattta aaaaatataa cggacttaaa agatgtatct tctaaattga catttggagc
    48241 tgaatatgac ttttatgaga gagaggatgg atatactcca ttgtgtaata catatggact
    48301 gaactttaaa gatacagttg atttagatat tggattaaaa taccaagcta tcaatgaagg
    48361 taagattgac gtaatgccta tatttacaac tgatggtcag tatagtgttt cagatgttgt
    48421 agttctaaaa gatgataaaa atttctaccc atcttatcaa tgtggaaatg ttgttcgttt
    48481 ggaagtatta gaggaaaatc cagaactatc aaaggtattt aagatggttg gagatatttt
    48541 gactgataaa gaaatggcta aaatgaatta tgacgtagaa aacaacaatt tagaacctag
    48601 agaagttgct cgtgagtttc tgaataagaa aggtctttta taaattggag gaaatataat
    48661 gacacctatt atacaattta aaaatattaa aaaacaatat aatgataaaa ctataataga
    48721 taatcttaat ttagatatag aaaaaggaga gtttttgact gtaattggtt cgtctggttc
    48781 tggtaagaca acactcttaa aaatgattaa tggtctgatt ttgccagatg gaggaaatat
    48841 tttgataaac caaacagata tcaaaaacga ggacttaatt aaattaagaa gaagaatagg
    48901 atattgtgta cagggaagtg ttctttttcc tcatatgact gttgaagaaa atatttcata
    48961 tgtaccaaat ctcctaagta aaaagaataa attagaagta aaaagtgctg taaacaagtg
    49021 gatggaaata gtgggattgc caaatgatat gaaagtgcgt tacccatcag aattgtcagg
    49081 tggtcagcaa caacgtgtag ggattgcaag agctttagct tcttctccag aaattttatt
    49141 aatggatgaa ccttttggtg cagttgatga gattacacga aagcaattac aaaaagagat
    49201 aaaagaaatt cataaaaaaa caggagttac aattatcttt attacgcatg atatttatga
    49261 agctttgatt ttgggaacaa agactttggt attaaatcat ggagttgttc aacagtatga
    49321 tactcctgag aatattttaa atactcctgc taaccaattt gtagaccagt tattgaatat
    49381 aagaaaatct ataatagatg atgataattt aaaagaataa atttaaattt aaaatcaaat
    49441 tgttgtgata aaaactattg atttagagct aacttcaagt tgtatacttg aagtggaaaa
    49501 gttagattaa ggtatagaag ggcatttagc agttggctgt agacgaggta actgctgaag
    49561 gtgctttcaa taaatggcta gaacctgtat ctgttgaaga ttataataaa ttgaaataag
    49621 aagctcaata tacagagtag ataggcactt caaatagaag tgtcttttgt gtgccataaa
    49681 ataaatcaaa atattagcaa agaatgtgaa aaacaaaata aaataatatg ctatctttaa
    49741 aataggaggg atgctatatg gataacaatt tgattaccat tgaaaaaact ttagattata
    49801 tagaaaaaca tcttttagat gagatgttag atttaaatgt aatttctaaa gagataggct
    49861 attcaaaata ccatttacat agaatgttta catcagttgt cggatttagt gtacataatt
    49921 atatacaaag acgtagactt actgaagcag cgagaatgct aatttttaca gaaaaaccaa
    49981 ttattgagat tgctttaatc tcggggtatg atacacaacg ctctttttca agagggttta
    50041 aaaaaatgtt taaacgtagt cctaattttt ttagaagaca tcaagatttt ttaccactac
    50101 aattaaaatt tgatattcat aatagaaaaa aattacgtgg agatatggtt ttagatgtta
    50161 aaaaagttaa ttgtgcagat ataaatattg ttggctattc tggtaaagta agaaaaggat
    50221 ttcatattat tggaaaatgc tggagggatt tgcataaaaa taaagagatg atagaaaata
    50281 aggtagattt aggtttttta attggagtta gtgattatag caattttaaa gagattgaga
    50341 atactcctac atttacatac attgcaggtg ctcaagtagc tacttttggc aaattaccta
    50401 atggaatgga aaaattcgta ttaccgaaaa gtaattatat tgtttttact tttattggga
    50461 aaaatgagga tagtatgcaa aatgttgtag aatatatcta tcaagaatgg ttccctaatt
    50521 caagttgcca gtttaatgaa aataatttat atgactttgt aaaatatgga gaaaaaactg
    50581 atgagaatgg tttgagtgaa attcagtttt gggttccaat catttaaaag aaggtattat
    50641 gtataaagta gatggattta tataggcaaa gttttacagc agtatgtatt tataaataat
    50701 ttatagtttg taggaaagaa ataattttac tataagatta tgataaaata cacaatacta
    50761 tgacaaaata gtgttaaata atagatttaa ttttgcatag gaaaagttct ataatggtgt
    50821 gtattcataa ataatttata atctgtagga aagaaatagt tttactataa gattatgata
    50881 aaatacacaa tactatgata aaatagtact aaataataga tttaattttg caaaggataa
    50941 ggtgttttaa taaaaatgaa tgtagaaaac gaattactta aaaacctaga tagattgcat
    51001 acaacagagt taggcgttgt aagaattaag aaaaatcttt ctttggagac aaatgatgta
    51061 gtgaactggt gtaaaactaa aatagaatct ccaaatgcca ttattaatag aaagggaaaa
    51121 aactggtata taagtgtata tgattgtatt ataacagtaa acgctcacag ttatacaatt
    51181 attacagctc ataaagaaaa gaaatgaatt gcaaaataca aaggtcaaaa gcattttcca
    51241 ctgtttgtag tacctatgaa gaagtatata aaataatatt acttttgaat aataatttaa
    51301 attttggagg ttgaaaaaat gaatgatact gtaaaagaaa aattggcaga gtcactaact
    51361 tctgatacta ctgatatttt accttatttg ccttatttat tacaagattt ttgggaatta
    51421 ggaagttcac cagaacaaat gttacaatta tttagaaagc atataccttt taatgaggat
    51481 acaaaagttt tggatttagc ttgtggaaaa ggtgctgtat ctgtgaagct tgcaaaagag
    51541 ttgaatatac atatcaaagg tattgatttg atacctgaat ttattgatta tgctagaaga
    51601 aaggctattg aatataaggt tgatgctttg tgtgattttg aaatgaatga tgctaatagt
    51661 tctgtaaaaa ttgagcatga ttatgattgt gtgatttttg gagctgtagg ggatatttta
    51721 ggtaattata aagaaactat aagtaagcta ttacctacaa taaaaagtaa ggggttcatt
    51781 ttaattgatg atgcatatgt agtagataaa tctacaaatg aacagcttaa ttttgagagg
    51841 gtgtatccca cttacaatga gtggtgtata ttgttcaaag aattaggtct gaagctaatt
    51901 gaatgtatac catgtttaga acttgaaaat gatgttgaag aggaaatgaa ttggattata
    51961 actcgtgcaa atgagctaat taaaaaatat ccttctcaaa aagatatgtt tgaacaatat
    52021 gttattagcc aaaaaaatga atatactgat ttacagcagg accttgttgg agcaacttgg
    52081 attttacaaa aagaataaga taaatattag actagataat caatgttata gtgaattatc
    52141 ccatgtctag tgaatataaa taacataaaa aaccgtcaag atgaatagtt tattcgtgct
    52201 tgacggtttt tagtatttgt tgaagtaaaa ttttatttct ttagtatatc acttttagtc
    52261 aatccatttg atttagcttg agtattccat tgatgattcc ccataagttt atataggaat
    52321 ctagggaagt taggtgaaat aaaaatagta cttttgttgg aatcatctat atgtatatta
    52381 tcactaagta tattttttga aagttctttt aatgcaattc ctaaatcttt tttaggtcct
    52441 ttacccaaag gtacggattc tagtgaacca aacatctccc cagctccaat tccaatacct
    52501 tgaacccatg taaagttgat ttttttacac caatgctcta tcatttgaat tgccaaatga
    52561 ttatgttcac cttcattgaa tccacagtta actattgcat acactggaat aggtttttta
    52621 ttttgtgttt ttaaaaattt ttcacatttc tctaaaaaat gaagtaaatg agatggaata
    52681 ccatctacat aaagaggaga tgcgattatt aatctatcac aattaagtat ggcattaaga
    52741 gcgtcaggat acttagatgt gttatctact ttaaatgtaa aaactttatt atcattttga
    52801 ataagttgtt ttaattcaga taaaaaatac tgtgaattat ttttatttac ttttggacta
    52861 ccatttatca ttactatatt catatggatt cacctcttat atcttgtata ttttcataga
    52921 aacaaacaga gtgttctttt atattgaaat ttatagcatt tccatttact aatttcgtag
    52981 cagtttcttt ttcagcttct gtaatacaat ctccatagaa tatagctttt aaatttatct
    53041 gtttatcata tctgctttta tgacgcatca tattatcttt tattacaaag tatggaagaa
    53101 tataattaat gcttttatca agtacgtttt tgacaaaagg gctaaaccct ccgtaacagc
    53161 atctgcttat tataataatt ccatcagagt catttataag tttacccatg ttgctgtaat
    53221 aatcttttac aacacattca gaaggagttt taatccagca accaaaacaa ccaatacatt
    53281 tgtggatttc ttttgtgttt ggaagtattt ttatattgtc atcaagtttg ggtaataact
    53341 tttctaattc tttgtcagtt aaatcatgta ttaaaagcct cataaagtcc atctcctttt
    53401 attatgtatg tttacttcta aaaattatat cttatagatt tttcagtaaa tctttacacc
    53461 aattaataac aaaattataa tagtcaactc cataaatcat tgttgcatac atatatgtat
    53521 attggtcaac acaatgattt tcttctgcat catttttttg tttacttaat ttatttagaa
    53581 gtaattctac ttcagttatt aacattttta agtttgtttc aattatttct ttgggtagat
    53641 acttgtaaaa gaatatatta acaaggtggt cttgctttgt tttagaaaaa tttataggtt
    53701 tctttaacca ttcaagaaat acttctttac cagtattagt tatggaatat agttttttta
    53761 atttgttacc atctataact tcgtgatagt gtatatatcc ttttgtttct aatcttttaa
    53821 gtgcaggata gatacttcca aagctggcgt caaaaaagta cgaggtactt tctgacatta
    53881 tttgttttaa gtcgtatcca cttaattctt tttccattaa aaatcctaaa ataatgtatt
    53941 ctaacatatt acctcctggt atcaattaga tatatatcga ttcgatatat ttattattac
    54001 agataataaa tcttacgtca agatgctttt taagataagc taaaaagaat taggagttat
    54061 tgataaatat gcaaaaaaat taatataaaa ggtaattaaa tatcaataaa gatttaaaaa
    54121 tagttaaatc aaagtaagag tctaatatat aaaatttata gtaactttat ggtttaaata
    54181 taaacttgtg tgctattgac aaagtataaa attagcagta gaataattgc atgagcaaat
    54241 agattgttgc gaacgcaaaa aaaggacaat agatattaaa ggagagaata tgagagagct
    54301 attaaaatgg gtttccatta taaatagata caatacaaat tatattgaca ggaaattggc
    54361 ggatattgga atcaataata gccaatattt ttatgtatta cacatatgta ataatccagg
    54421 tattacacag gacactatat ttgagaaaat tcttgtgaat cctagtaata taacaagagc
    54481 tttggcaaat ttagaaaaag aaggctttat aacaagagaa ccaggtgtaa aggataaaag
    54541 aacatggcat ttatatccta cagaaaaatc tttaggatgt tatgatgaaa tactaaaaat
    54601 aacaaatggg tatgttgagg agttgttgag ttcatttgag aaagaagagc aagagttgtt
    54661 tatgtcaatg ttaaagaaag ttgcattttt ggctattgat atgaatgaag aagcaaaatt
    54721 ggaggcgaag gaaaatggaa gaaaaatata aggagccatt ggaatatgaa aaggtttcaa
    54781 agttattaac taagtacgca attcctagta ttatagcaat gttagtaagt gctttatata
    54841 acattgttga ccaaatattt attgggcaag gtgttggtgt attgggcaat gctgctacaa
    54901 atgtcacttt tccactaaca acaatatgta ctgcaattgc attattgtta ggaattggag
    54961 gagcgtctaa gtgttcattg gaattaggtg ctaaaaattc agaaaaagct acaaaagcag
    55021 caggaaattc tatatgcttg atggctgttt ttggaataag tttatttata gtagtatcaa
    55081 tatttttaac accactatta aaattctttg gctctacatc agaaatacta ccttatgcac
    55141 acacatatac aaaaattaca tcaattggat taccatttct tataatgtcc actggaatga
    55201 gtaagttgat acttgcagat gggagaccga aagcttctat gttttgtatg ttaataggag
    55261 cagttattaa tacaattttg aatccattat tcatttttgt ttttaatatg ggaattgctg
    55321 ggtctgcttt agcaactgtt attgggcaac ttatatcttt tggaataagt gttcattata
    55381 taaagaattt tcaacatatt aaattacata agaaaagttt taaattagat agtataattt
    55441 ataaacatat atttgcgcta ggttcaagct catgttttaa tcaattagca atgactgtaa
    55501 cacagatagt tatgaataat acattggcat attatggggc taggtcagtt tatggaagtg
    55561 agattccact tgcttgtgtc ggtatcatta ttaaagtaaa tatgattttt atatctataa
    55621 ttattggtat atcacaaggt atgcaaccaa ttatagggta taattatggg gctgaaaagt
    55681 ataattgtgt aaaagaagca tataagtttg cagtgggaag tgcaactatt atatctgtta
    55741 ttgcattctt atgtttccaa ttattcccaa gacaaattac gtctatattt ggtaacggaa
    55801 atgagactta ttttatattt gcagaacatt attttagaac tttcttgttc ttaacattta
    55861 taaatggtgt tcaaatagta tcatcaactt tctttacatc aattgggaaa tcagtgttgg
    55921 gactttttac ttctttgact cgtcaaattt tatttttaat tccattgata gtaattttac
    55981 caatgtttat gggaattgat ggagtaatgt ttgcaggacc aatagcagat gcagcagcgg
    56041 caatggtatg tatatacttt gcagttcatg aaatgaaatc attgactata aagcaaaata
    56101 atttgatgag taaccataat aagattgcag aggtataaga caatatttaa gaagtataga
    56161 aaattaattt aaagaagtat aataaactaa tttaggaagt atagaaaatt aatctaaaga
    56221 agtataataa actaatttag aaagtataga aaattaatct aaagaagtgt aataaactaa
    56281 tttaggaagt atagaaaatt aatctaaaga agtgtaataa actaatttag gaagtataga
    56341 aaattaattt aaagaagtat aataaactaa tttaggaagt atagaaaatt aatttaaaga
    56401 agtataataa actaattcag gaagtataga aagctaatct aaagaagtat agtaaactaa
    56461 tttaggaagt atagaaaatt aatctaaaga agtataataa actaatttaa gaaatataga
    56521 aaattaattt aaagaaatac aatacattaa tttttaagta acattaaatt aatgtaagta
    56581 aaataatgtt catatgaaga tttaaaggtg cattttctga agctaagtat atgttgtaga
    56641 gtgttaggtc tctctatcct gtgaagttta atagctcaag aaaatgcact atttatgatg
    56701 attattttgt tttatttcca attttggaaa taactattct aattaaatct aaaattatac
    56761 cataaataag aaaagaaagt atagatgcta tgtaccaata aatcgaaatt gaccctgtaa
    56821 ttgttctaca tgggatttgc atacctgaat ccataaaaac aaaaaaacta tcaactctgg
    56881 aaataataga caaaatagga tttaaaccta ttagaacaat actttttgag tcttgaccaa
    56941 tttgatgcat ataaatctca aataatgcaa ttaaaaaaaa ccaaaatgta aaagatttaa
    57001 taattttttt catcaaaata cctcacataa tataaattct gtttgcacta tttgcttact
    57061 acaactattt atctccattc agtcacatca cttaattgta atctaggtct tcttgatggt
    57121 tcttcatcag gataacccaa agatattgct gccactaatt gactatctgt atttaaccat
    57181 tcacataatt cacgatatgc aaagtataca tcacaaatcc aaagactacc tagacctaat
    57241 tccacagctg taagcgacat attctgaatt gcagctccaa ttgattgcat gtttgccatt
    57301 tcatagaatc gttcttcagg ggatagcttt tctaaaggtg atttacctaa tatatttaaa
    57361 ataaaaattg ttactggagc ttgtttcatg atttcaactg tgtaatttgc accagcgata
    57421 tgttgacgac ttcctggaag aagcccatta tcattaattt cattttgaat acctttgctc
    57481 atggctttaa gcatagattc tttttccttt tcagtgatga caacaaaacg ccatggttgc
    57541 ctgtttttag atgaaggtgc atttattcca gcttcaatta ttttctctat agtttcatgt
    57601 gaaatgcttt gatttttata ttttctgata cttcgtcttt ttgatataga atcacttatc
    57661 attttaagta acctccgttt attttttgga aaaatctatt taatttttat tttctatttt
    57721 aattatttta gcattaatca gaaaaaaagt cattgatttt agtatacaat tattaatgat
    57781 tatattaaga attgtatcaa aaagtatgtg attatactaa gggattatat taaacattat
    57841 atttaaaatt gtatgaaagg ttgtatttaa tattatattg agagatatat atattaggat
    57901 ttatatatat tcagatttat tattatattg agccttatat taaattaagg actataattt
    57961 gaaaataagc tataaaaact taatattgtt ttgttcagag ataaaaaaag aatactttag
    58021 gtgattttta ttaaaaatta agagtgataa aattatatac tttataaatg taaatatgac
    58081 gaataaaaat gataaatata ataatatata tatttacatt aggagttttc tatactataa
    58141 ttatttaaaa ctaaatataa atgttggaat tatttaggac ttgaaattgt ctaaaaataa
    58201 atacaagtga tgaagaggtg atgtgatgag attagatatg gatagtatgt tatatgtact
    58261 aatttatatg acattattgg tatctatcat tggaagtata ttttattttt gtaaagacct
    58321 cttaaatcat aaaattaagt atgttttaat aagagacata tttgtaaata gaattacata
    58381 ttctgaagaa cgactgccta aacagtatat agtttttcag aaatatgata tttggaggta
    58441 ttgtagtttg ttttaatttg aaatgaatct attataaatt ctaaagttta aaaataaaaa
    58501 ggagagaata aaatggattt catttcaagt atattgcaag gattattaag tatattattt
    58561 aattttacag gtgattttgg aatttctatt gtacttataa ctttaatagt taaattagta
    58621 cttatgccac tatcattaaa acaaagattt tctgtaaaaa aacagcaaga attagctgaa
    58681 aaaatggaat atataaaaga aaagtataag aatgatacta aagaattgga aaagcaaata
    58741 caagtaagtt ctgtagaaag tatgaagagt atgctgggat gtcttatagt tttggttcaa
    58801 atgccagtag tatatgcttt atatcatgta tgtttagata tgacaaagga atttacaaca
    58861 attataattc catggattgc taatttaggt acatttgata atttgtttat attaccttgt
    58921 atatatacat taattatgtt agcttcaaat ttagtgaatt atataccata tttaaaaata
    58981 aattctcaag taaagtttaa caaacaaatg gctgtggtga ctaccataac aagtcttata
    59041 ctaactgtta gaactccagt agccataggt ctgtacttta taacaagtgc aatttattca
    59101 ttaattgaag atatttgctt tagaatatat tttaatcgaa aaaatagaat agtgacaaaa
    59161 taatataatt attattagaa atattttagc ctgctaacat agaaaaagtt agtaggctat
    59221 ttttatctat gatatcaatt ccattaagaa tagtctaaaa aatactatca aaatgctata
    59281 aaaaatacta taaaaaaata gttgaaaaat aaataaatta atataaacta aaataaagcc
    59341 tacaaaaaag aggtgataaa tagaaatggc taaaatacta gttgtagaag atgaaaaaag
    59401 aatgcaaaat attattgtag aatatatgca aaagggagga tatacttgta taactgctga
    59461 tgatggagtc gaggcactta caatattaaa gagcaacaat atagatttaa tgatattgga
    59521 tattatgatg ccgtatttag atggattttc agtgtgtaga gtttcacgtg aaatgactaa
    59581 tatacctatt attatattga cagctaaagg tgaagaggag gataaattaa agggttatga
    59641 atatggcgca gatgattata taacaaaacc atttagccca aaagtacttt tagcaaaagt
    59701 taatgctttg ttacgtagat acactactga tattcccaaa aattcattga gtttaggaaa
    59761 gatttttatt atggtagcat caagacaagt gtatgtagaa gataaattga ttgatttaac
    59821 atataaagaa tttgaattat tgcgtttatt tatggaaaac cctaatcaag ttttttccag
    59881 agagaaactt ttaaattgta tatggggata tgattttgaa ggaaacacac gaactgtaga
    59941 tacacatata aaaaccttac gcaaaaagct tggtagtgaa ggacaccata tagttacatt
    60001 aattcgttca ggatataagt ttgaggttaa ggaatgaata gtttaagaaa gattatagaa
    60061 aaactgaaaa atgtagatat ttcaaattgg attaaacaaa attttggact atattcagtt
    60121 agaaaaaaag tgtttttact ttctaaatta tcaggagcgt taattatatt attttatctg
    60181 gttgttgaag aattttcgat aaactctaaa tttgaatttt ggatttggtt tataatactg
    60241 gttatattta ttatgagtat tgatttctta ttgggaaaat ttatttcaga accaatcaca
    60301 tctataaata aatctgcaaa aagtatgtcc caacttgatt tttctaatcc atgtactgta
    60361 aatactaatg atgaatttgg agaactttct aggagcctga atacaatgtc tacaaatcta
    60421 caacaggcat tatcagacct agaaagtgca aatattcaac ttgaaaagga tgtaaataag
    60481 gaaagaatgc tattggaaca aagaaaggag ttggtagata caatttcaca tgaaatgaag
    60541 actccacttg gcattatacg tgcgtataca gaggggctta tagatgaagt tgatgaagaa
    60601 aaaaagaaaa attatatgaa tgttataata gaagaaactg atagaatgaa caatatgatt
    60661 gtatcattat tagacttatc tgcattggaa gcaggagttt ctaaacttaa cccagagcgt
    60721 tttgatttta tagagtttgt tgaaactgtg gcaggtcgtc ttttaattga tataccagat
    60781 gccaattttt attttacata tgatttgcca gagtatgaag tttttgtagt ggcagataaa
    60841 atgagaatgg aacaagtagt agagaatcta gttattaatg caaagaagca tgttgtatat
    60901 aatggtaatt tagatttatc gattacatgt gaaaatggct tgcttttatt tgaaatttat
    60961 aatgatggta ggtttataga gcaagatgag atttcaaaga tttggtctaa gttttatcgt
    61021 agtgttcaat ctcagcgtac aggaggttca ggtctagggc ttgcaattgt ttcacaaata
    61081 cttacaatgc aagggttaga atatggagtg aataatcgtg atagaggagt agagttttat
    61141 tttatgattc caattgatga ataatttagt attaatttat aaattataag tatctgatgt
    61201 aaaatatttt tatttgtata cgtaagtatt taaagagaat aaaataaaaa aacttaatga
    61261 aaaagcttaa tgaaaaagtc taataaaaag cttaatgaaa agcttaataa aaagtcttgt
    61321 tttcctgatg aaaacaagat tttttattaa gctttttatt atttcacttc gatttcacaa
    61381 gagagacact ctgatttcac caaatagaaa taaaataggt taaataaatt aaaaaataga
    61441 agcgtaatta aataaggtta caagggggag tacttatgaa atggtataca tggttgacta
    61501 ttatattgat aatattgctt tctattccgc ttaaaattaa atttataaaa tggtgggata
    61561 gacgacaaaa aaatgataaa ggaggttatg atagatgata aaagtaagag agttaaaatt
    61621 ttcttatgga aaagataaac agatattgca tggacttaat tttgaagtaa aagaaggaga
    61681 aatctttgga ttccttggac ctaatggttc aggaaaatcc acaacacaga aaatcttaaa
    61741 tggtgtttta aaaggttaca atggtcacgt atccattttt ggtaaggaag ttaaagcata
    61801 cacagaatct ttatatcaaa agattggtgt tttatttgaa tttccttatt tatataccaa
    61861 tttaagtgca attgataatt tagaatattt ttcgtctttt tatcctaaaa atcaaaggag
    61921 agacatatat gaactcttag atttgttaga gtttaagaaa gagtttataa ataagcctgt
    61981 atcatcctac tcaaaaggta tgaagcaacg tattagcatg gcaagagcat tggtaagtaa
    62041 tccaaagttg ttatttttag atgagcctac tagtggactt gacccatctg gagctgtact
    62101 atttcgtaag attattgaag aagaacgaaa aaaaggaaca actatctttt tgactactca
    62161 taatatgcta gatgcagatt taatgtgtaa caatgtggct tttattgcag atggaaagat
    62221 tatggtaata gataaaccca aaaatttaaa aatgaaaaat agcaataata aagttgaagt
    62281 ggaatttgta tataatggaa atcgagatat tgaaattgta gatatacaag agttagaatc
    62341 tggaataact tttaaatatg atgaaatctt aagtgtacat tcaaaagaac ctacactaga
    62401 agaagttttt attaaataca caggaaggat gttagtgtaa tgtattctaa attttttacc
    62461 ttagtaaaaa aagatttttg gatgatgatt tatggtaaat tttttgtgct gacacttggc
    62521 tctctcatac tttattcgtg ttatattaat tttggttatg taaagtttat ggatgaagat
    62581 agttatcaca ataatgtata tctatataat ccagagaata taagcatgag tgcatctcca
    62641 ttaattaatc aagtttcttc tataggtgaa ttaaaagcaa aacttgatga agatacaagt
    62701 gcagtttgta ttgatatgag ttccagtaag ccgaaagtaa ttttacatga aagtattaaa
    62761 ggagttgaca attatagagc tgactatgca ttgtcattat tatcatcaga aaaagaccat
    62821 aatagcaaaa tatcagacaa caatgcaata attcaaatta atgataagga tacatctgag
    62881 ataaaacaaa gaaaagacat gacttgtgag attttattct ttgaaattgt agctattgga
    62941 tttttaggga ttgcctcttt attatttaaa gaaaagcaga tgggagtaat gcgtattact
    63001 ggtattttac ctgtaaaaag aagtcttttt atattatcaa agatatttgc tttttttata
    63061 tgtgacctat gttttacagt tcttttagta gtatttaata ttggattttt agaaggaata
    63121 aaaatacttc cacaagtatt agttcaagct agtattttat caattattat gtctttgatt
    63181 ggaataggat gctcaatgtt attgagagat tttaagcaat tttctcttat atatcttgtg
    63241 gttgctgtat tcataactac acctatattt ctatctgcaa atacatcttt tgaaatagat
    63301 tggattaaat atcatccctt ctatcatgtt tatagtcagc ttaaaaatgc gtttttcatg
    63361 aatttttcaa aagaattagt ttattttgta gtgtgttctt tgacaataat agttctattt
    63421 ttacttgtaa aatatatttt taataaagaa ttggttaggg agggataatc ttgataggat
    63481 tacgttacca gttaaagagt ataaaaaaag ataaaatgtg tataatttcc ttctttttac
    63541 cagttgttat ggcgatttta ataaatttta tgggaacaat tgatatatct tcattaggag
    63601 agatacgttt tggtatagtg aaggatacac ttcctgtaca aacagagcag tggttaaaga
    63661 agtatggtat ggtaagtgta tatgacacaa aaatgcattt aatacgtgaa attaaggata
    63721 ttaaaacgga cacaattgga gttgttagag atggtaaagg aataaaaact gtgttttatg
    63781 gaaatgagat taatgctata aaaaatgttg gaaagacatt gccagagatg tttagactaa
    63841 agggtgattt aaaagatgtt gaggttacaa tattgcctaa agatagtatt ttaaaagagt
    63901 ttcaaaatgt ttttattgct atgattttaa ttactgctat gtttatggga tgcaccttta
    63961 atgcaatgaa tattatatcg gaaaaagaag atggaatatc atttataaat gaagttttgc
    64021 cacagagtag aagtcagtat attatacaaa aagtattttt gggtctttta tgtgggtgtt
    64081 tatcttcaat gataactgta tttatatgtt tggatatttc aatattagat atgataatga
    64141 tgttgttact cgttgtattt tcagcattta tttcatcact tataggttta tttattggaa
    64201 agttgtcaga aggtcttatg gtaggaattg tttatattaa aatagtaatg attttattta
    64261 taggtactcc tttgttaata tatcttctag gggttaatac cagtggtata gggaatttat
    64321 gctatataat gccttcagtt gctacattta atggtattat ggagattatt aatggtaatg
    64381 tcattattat caaagatatt gtaattttgg ctgtacattg tgtagtatgg tttatgatat
    64441 atattatttt agataaaaaa agaaagttat cttttagtag ataactcagt ataattataa
    64501 ttgggacaat ttaataggtg atttaatata ctttgtatag tgtaatttat gtagtgcaat
    64561 ttgtatactt taatttatgt agtgtaatag ttagttttat tttataatta ttcaatataa
    64621 tttagtaaga agttcagtac aattagagct acttagtgga aaacttaata ggtacttaaa
    64681 ctatataaat actttatttt cctccatttg tactataaat ttaatttatg atataaatgg
    64741 aggtattatt atatgatttg ataaatttca tcattataac ctacaagtaa aggtattatt
    64801 atataaagtt aataaagata gtatcaaaat attatttgct ataaagagag ggactcaaaa
    64861 taaagataaa cctatttttc aatgttgaaa tgataaaata tatttgattt catataaaaa
    64921 attttcttca taaattaaaa agtccatttt aagccccttc atttttaacc agataaatcg
    64981 agttaaacta tttaaatata ttactcggat tcatttgact tagatattcc ttgcttgtaa
    65041 aattgttttg aataggggat agccaaagca aaaaataaaa gtataatccc tgctatgatt
    65101 attaaattat taatagaaat tacatctgcc attggtccga aaatcatcat accaagagga
    65161 ataaaaccag tatatataat aataggtaag ctaagaactc tacctagcat ttgtggtgct
    65221 acatgctctt gcaacatagt catagctgat gcttgaatga ttggaattga aaaattcgta
    65281 aaaaacataa atataaaaaa ttgccaagct tgtgttacaa gacctagaat aattgaaaaa
    65341 atagcatagg ataatattgc tagaaaaaag gtcttatttc tatttttaaa accaccaaaa
    65401 actcctataa tcaatccacc taaaaccata cctaaaaatc caatcatttc aataatagac
    65461 aggtatatat aacccttgcc aaatgttcgt tcaataagaa gtcctataag aaatcctgat
    65521 ggaacacaaa gaagcatgaa tgctccataa gtaaaaagca cttttctaat gaacttatct
    65581 gaaaaacagt actttatccc tgataccata tctgcaaaaa atgagacttt ttcagtttgt
    65641 tttggtatat catgtttagg aattttgatg aatattaata atacaatagc aataattgca
    65701 gtaaatacat caattaaaag aacttgattt attttattac caaatccaag tattgcagca
    65761 gctacaatag gaactataaa ttgtactata gactgtagac tactatttat accattaaaa
    65821 cgcattaaat gttcttctgg tacaagttga ggaatcattg cgtttacagc aggtgtttgt
    65881 acaccacttc ctagtgaacg tataacagat attactgata tggctattaa ttcacttgtt
    65941 gtgccatttg atgacatcat aaatagaatt agaactaatg tagatatagc aattgttgaa
    66001 tctgatacaa ttatgagtac ctttcgattg tagtagtctg cccatacacc agcaaaaaaa
    66061 gaaattatca tttgaggtat aaaggagcat acagttaaaa ctgttaccca tacacctgat
    66121 gatgtctgaa gtgtgacata ccatatcatt gccatttgta caagtgatga gccaaataag
    66181 gaaatatatt gacttgctaa aaaaagaatg acttttttct tccatgtaga attagtattt
    66241 ttattttcca tcctattcct cctttgattt gtgtgttgaa tttttcttgc tcttaaggat
    66301 aacacacaaa tcaaagtctg gagttagagt ggagggctag gaaaattgtt ttttcataat
    66361 ttgaagatga gatttcatgg tttttgcatt atgttcagct tctttcaatg aagttatcag
    66421 tttgtcacac actgagataa tttcgtcatc atcttttgga gtgtcaagga cagttttaat
    66481 attagtatta ggactagaag ataaagcatt taacattcgt aaaatagctg aaagagaata
    66541 gtttgcacaa cgaagtgcgc ttataatttt aagtcgtttt atatcttcat cagtatatac
    66601 acgataacca ttttgttttc tttttacttt taacaaacca ttcatttccc aatttcttaa
    66661 agtatctatt gtaacgttta agtgttctgc agtttctttt ctagttaaaa agagtttatt
    66721 tgtttctgga gtatttccat ttaaaatctg tgcaacaata gtaagtgctt cctcggcatt
    66781 agatttttct atttctaatt gttgcaagta ttgatttaca agactaatag ccttatcaaa
    66841 gtccctattc gctgatgttc ttacaatctc aagggcatct tttcttaatc ccctttgtac
    66901 cacttcaatt ttcaaggctg tacgagcaaa tctaaattgt tctatatgaa aatcattaaa
    66961 gatacgataa ccattggtat tacgcttagg tttaggtatt aagtttagtt cttcatataa
    67021 acgtactgta ttaggatgta caccaattaa atctgctacc tctgatgtcg tgtaagtgtc
    67081 cattagtatt caactccttt tagatatata gtattagtca taataacacc aaacaaaagt
    67141 ctggtgttat aatggagggt ttggctatct aagagtaatt agtttatgaa agagtaatta
    67201 gtttatgaaa gagtaattag tttatgaaag agtaattaat ctattataga agtaagtata
    67261 tatttttcaa tgaactccca tctatcacga ggtttaaaca ctattttaga tggtatggct
    67321 attactaagt catattgtgg aatacaacaa atagcatttc ctccagttcc cattgctgaa
    67381 taccacaaaa caccatcttt tttacatagc caccacagat aaccgtattc attactattc
    67441 attttaactg attcttcaat ccacgattta gatataatac gtttattatt ccaaatacca
    67501 tcatttaaat acagaaaacc aaatcgcaac atatctctag cactcaatgt aagtccccag
    67561 ccacctatag aataaccctg tctatctttt gcccaaccct taagattttt tccaaagaaa
    67621 tcttcaaaac caaaatcttt catatcataa tctggtataa ctttcatacc aataggttta
    67681 aataagtact cattagcaaa ttcacgagca gattttccag tagtttttgt tataatagct
    67741 gaaagtaggt gcgttccagc tgaagaatat ttgaaagtac caatttcgcc tttttgacca
    67801 agttgattca aggtatattt tatccaatct ttttgtttgc aaagtctttc taaagactca
    67861 gtcatatttt taaaagggta tggtgcagtc attgtaagta ggtgacgtat agttactgct
    67921 tttttacata tttcagaagg actacaagta tattcaggaa aaaaatcaac aaccttttgg
    67981 tcaacatctt ttatatatcc tttatctatt gcaataccta ttaaggctga tataatactt
    68041 tttgtaactg atgcaacatg aaaagtatca tcagcattat atccattata gtacttttca
    68101 aagacaattt ttccttttct tgcaataatt ataccattta tattgctata ttgtgagatt
    68161 attaaaggtt caagttgtga aagcttttct gaactcatac caacacttga agggtctact
    68221 ttttcaaaaa ttaagtctaa gcaattgttt ttattcatta aagatacctc cctgttatag
    68281 attttggatt ttcttttaga gtttttggag aaccttctgc aattatataa cctcctttgt
    68341 caccaccttc tggaccaagc tcaacaatcc agtcacaaac atttaataca tctatatcat
    68401 gttcaacaac aattacagaa tttccatttt caaccaattc atctaataat tgaatcaact
    68461 tagaagtatc atatagactt agaccagtag ttggctcatc caatacataa aggatattac
    68521 catttcgttt tttaccaatt tcttttgcaa gtttgattct ttgggattca ccaccactta
    68581 aagtcggtgt aggttggcct aattttatat accccatacc tatttgttct aatacattta
    68641 agtgtgaaac aatatttgga atatctttga agaaatgtac agattcagaa acactcatat
    68701 ttaaaatatc atgtatattt ttatttttat atttaataga taaagattca tcattaaatc
    68761 tttttccctg acattcactg catatatggt ctatacttag attatttccc aaccagacct
    68821 tttcatatcc actaccacta catttaggac aagccccttt ggagttaaat gagaaatgac
    68881 cagctgtaaa tccttgttct aacgattctg gctgacctgc aaatagtgta cgtatcttat
    68941 cccatatacc aatataagaa gcaggagttg agttcatatt tttgcctata ggagattgag
    69001 atatctctga atatccagaa atatgtttag taccaatcag tctatctgca atagtttcaa
    69061 caactgcata atcgtcttcc tcacctattt cactctctga agaatttatt tgattattga
    69121 tagtatggtt attaaattgg cttctaagaa gaggaagtag ggtgtctgat ataagtgaac
    69181 tcttgccact acctgatttt ccagatattc caaccatagc acccaaaggt aatgatacag
    69241 ttacattttt aagattattt gtatttgcat tttgaatagt tatacaaggt attttatcag
    69301 tatgtgtaat gtttttattg cttgttctat taggcattgg atattttcca gatagatact
    69361 gtccagtaag tgatttgtcg gattgaagta gaccttctaa atctccttga tatactaatt
    69421 gaccaccttc aacaccagct tttggaccaa tatcaataat atgttctgcc atttcaattg
    69481 tgtttttatc atgctcaact actatgaccg tattgcctaa atctttaagt ttctttagtg
    69541 attttaagat gtctattttt tctgattcat gcaatccagc tgttggttca tcaagcacat
    69601 aaattaatga gtccatctct gaatcgagat gtgaatttaa aaatagtctt tgcatttccc
    69661 caccactcaa agttgacatt tctcggtata aggatagatg acctaatcgt gattctatta
    69721 aatgttgagt tttggataaa atatcattta caagatttgt accaaatgtg tttaaattac
    69781 cttgatttat taagagttta aggaaatcat caatttctaa aatcgtcatt tttccaagtt
    69841 ctccaatatg ttttccgttt aaaagtactc tacgtgcttc ttcaccaatt ttaaaaccat
    69901 tgcaatctga acaggcagtc atagtataaa ctccatttaa atcttcgccc cttttgtaac
    69961 gattgtgaaa tacctttgat aaagaggaac tttttttacc attttcataa tgaccataga
    70021 gtacttcatt tcttatttct tcaggtagtt gtaagaattg tatgtctaaa taatcattga
    70081 attttttaga aaatactctc aaatatccag gtgtaatctt tgctctatct aagacttgtt
    70141 ttagggtatc atgcttatca aggactaatt tttcaatatt aatctcaaaa tatgcaccat
    70201 gtccagagca cttcatacac attccatctg aagagttgta tgagaaataa cttggtctta
    70261 atcgttcttc ctcatatcca caattattgc aaattagatt atcgcttaca ggagtatcac
    70321 acatagtaca cattatttga ccttccactg aatacaatag tgtaagcatg ttgagaatat
    70381 tggttcttgt gcctactgtt gaacgaggat tgctctggcg aataatattt tgttgaacag
    70441 ctatagtggg agctaaacct tgaatattat caaacttatc ttcactatca atacctgcaa
    70501 gtatacctaa agattgcaga tattgtttac gcccttcctc aaatacaatg tcaaacataa
    70561 ggctagactt acctgagcca ctgacacctg ttgcaacaac aagcttatct tttggaatag
    70621 aaacatctat atttttaaga ttgtgtatct ttgctccatt aatttttatt tctttcattg
    70681 gttcacctca ttttatcaat tttatcaaac cgtaaaatag ggatatatta atccttaata
    70741 caagaataga ttaggtgagg ttattaaaaa ggttctatag gtaaatatat atcgaccaag
    70801 tgtctatttt gagggtgatt gtgtgggttg ttaagatata cttcaaaagg gaaggaggca
    70861 cgtagcttat aattaccatt tgatagccat tcattataca taaaatccca cgcatcacta
    70921 tattcagatt ttgaaatatc aaaatgtccc acaagatatt ttccagaagg tattatcata
    70981 ttgccaagat tgtcattctc tttgacaaca atgttatctg gaatggtcat gcaaatacta
    71041 gtccttaatt gatgctcctg tgtaaattgt ggattatcat gataaatggc taatatttgt
    71101 gtgttttcaa gtccaagtag attctgctca cttgcatatg tgaataaccg ttctaaaagt
    71161 tttgggaaag tactggttag attaccatat gaaccagtat atctagtgta aatagtattc
    71221 atattatcaa gttctagaat ttcaatttca cctttaacct ctctagtatt cttacattta
    71281 ggttttgata tgcttctatt gtactgagaa attttatatg gttctttgca attcttgcta
    71341 taattgtttc tatattttat agggcttatt ttatagtaat ttttaaatgc tctcgagaaa
    71401 actgctgaat cagtaaagcc gaagtagtgt gcaatatcag taacagtcat gtcagttcta
    71461 tgaataagaa aagcagttgc attttctagt tttagcctat ttacatattg tgccaaagtt
    71521 tctttttcta ttgctttgaa gattctatga aaatgaaatt ttgaaaaacc tgctatatta
    71581 gaaagttcat caagagaaag aggttcatgt aaatgttctt ctatatagtc ttgcactttg
    71641 caaatacgat ttagatattc ttttttactt ttagcatttg tcatatatag ccctccttat
    71701 tgtgtaaaat gtgtatcaat taatttatag caatatagta ataaaatttt atgtttttgt
    71761 aatttatgat aaataagata gcgtttaatt ttttataatt gataaaagat ataagatgcc
    71821 ttatatttgt ctttgcatat aagtatagtc ttcgtaataa ttagtatacc atagtgattt
    71881 atttttaatt gtgtatttat ataaatacac aacttatgat atggacgaat tattatgcaa
    71941 ataagctgta attaaataaa ttgcaatata aaccagttga aacataagtt agggaaatat
    72001 tatcaaagtt attacttagc ataagagtat agatggaata aaataagatt aaaaaattac
    72061 atactaaatt taataaaatt tttatattaa aaataatatt aagtttattg tttaaataaa
    72121 aaagttaaat ctacactatt aacttattat acttaaaact ttaaaatgaa aatatcaaag
    72181 aatacttttt aataaaaata atcatttaat aagttattta aaaatctaaa aactttacaa
    72241 caaattatat atttaaattg gaggagtggg aatatgagta atagttttta tcaattaaat
    72301 tcagcagtat tatattttaa tgaaaaaaga gatgtaaata gaataaggtt taatcactat
    72361 gtaatgagaa aagaaaatat acaaagatta aaaggaactc tgccaagagg acaatttatg
    72421 atgacagtaa ggaaagctgc atctgacctt gatgtatcta tatctacaat aagtagactt
    72481 gtaaatgaat ttatagattt aggtatattg agattaataa gtaggggagt aaagggaaat
    72541 tgttgttctg tatatagtta tgtttgttct gaaagtagta atgcagaaat tgtaacaaag
    72601 aatggtgata ttttcaaaaa tatagagaaa tacattgatg caactattaa caataaccaa
    72661 aaaacaatga aatataaaca tagaaatgag ttgtttaaga acagtaatac caacatatac
    72721 agccgtataa atcaagagag tatagacaaa gattatgatt tgataattca tactgggtat
    72781 gaaacagaaa atgagacaaa gaaaaaagaa ttattaaaaa ataatttaaa taaaaagaat
    72841 ttaaataaaa atacctttga aaattcggat gaagatttga ctgaagattc tacttacaaa
    72901 aatccagaaa gcatacatga aattattata agaaaattaa atagtgaatc aggaaaaaat
    72961 tttaaagctg atttgagtat aactaaggca cttatagata aaagattaaa agaagggtat
    73021 agtttagaag atttttacaa ggttatagag gcaaaggtaa gaagatggag atatacagat
    73081 atggaaatat atatagaacc aacaattctg tttgggtatg aatttgagtc gtatttaaat
    73141 gaggagagtt ctttatctgc ttgtaattat ggtgatatgt gttatgaaga tatgtattga
    73201 gatatttatt aagatattag ttgcttttta gttgtttaaa atatgtttat gtataaatag
    73261 tgtattaaaa tggagttgtc tcaaaataga gagaagtctc ttcttaattg tttaaatgat
    73321 gaaaatatat ttgatttaat acaaaggaga gtgctttatg agttagaaga tttattttga
    73381 gacactcact ttttttaata tactggagaa atataaccca ttttacaaga gtgcatagat
    73441 atatataatg gcataatgtg gatttaaatt aaaaaaataa aataaagcca taaatattga
    73501 aaatttcaat atttatggct ttattgcttg taataattaa tagaatatat attttttatt
    73561 aatcatctat ttatattttt tattaatcat ctatttcaaa ttctaatatt ttaccagtaa
    73621 ttgcattaat tgttatctca tattcagttt ttccttttat tatttcacct tcatattctt
    73681 tgtcatcttc atcatactta aattttacta ttttcccacc aggcacttta tctagcatta
    73741 ttgatttagc tttttcagca ctaataatag aactgttgtc attagtatta gagttattct
    73801 tatcattatc atcaaagtca tcatcagaat cattgtccat gtcaatatct acaattcttc
    73861 catcaaatgc acttatctct aattcatatg ttttcttatt ttttattact ttagcctcat
    73921 attcgttatc ttcagcatca tatttacaat agattaaatt accattagga atagcatcta
    73981 atgctataga cttagcttta tccaatgata tattttgtgt acctaaatta ttatcattta
    74041 agtcatcatc tttttcaatt tctattattt taccagagta ggcatcaatt tctatctcat
    74101 ataggatatt atcctttata actttgcagt catattcatt atcatcttca tcataataac
    74161 aaactaaaat attgcttcct ggaagagctt gttttaatat ttctttagcc tgtattaaag
    74221 atattttttt agttttattt cttatgttca cattttttag ttttaaaata tcttcaaatg
    74281 ctttttgatt atatcctcct cctacttgtg ttatttcatc tatatcttta tttttaataa
    74341 attctttttg actatttaca aggctaccat tagatatcat tataggagaa ttttctttag
    74401 cagctagtac tcctattgat aatgaatcta ctaatgcatt ttcttggtta ataccatctt
    74461 tagctacaaa tacattattt atttttttat ctttataaaa tttatcaata atttttgcat
    74521 tagtattgtt tctatctgaa ccactgattc tttctctgtt tggatatttc tttactgtat
    74581 tgtctgataa aatcttattt cctccaacta tgtaagattt ttttatagac ctattgttta
    74641 agaaatttat actactatca ttgctgtttt catctgatac taatattata ggcatattat
    74701 gtattgctgc tggagaagct atactaactg catcagatag tccctttagt ccatttacaa
    74761 ctgcaacatt ttctatcttt tttatcttat ctatttcttt aagtacattt gtagacattt
    74821 catatatatt gtttcctact attttattta tttttatgtt aagtgatttt atttcatttt
    74881 ctactttaga agaaatgcta tttccactat ctacaatgta cacattatct acaccaagcc
    74941 ttttaagctc agccttagtt gaattttcaa tactgttgtt gtttactaaa agtataggtg
    75001 cattctttag cttagcaaat ggatttgcac aaagcgcact ttgaatagaa ttaccattaa
    75061 ctataatggc tgtttttgat tgaacccatc ccaatttact tacttctact gctgtttcat
    75121 atcttgtttt acctattaat ctttcttgct ttgtagtatt gtctgcaaaa attgctgaag
    75181 aactagaaaa aaacatcata cctgatatta atatagctaa atttttctta ttcatatttt
    75241 tacctcacaa tctttaaaaa tttgttattt agcatactta ataaaatata atcttaaaat
    75301 caaattgata ctgagtttga agttacaaaa tcaacaaata agatttcttt aatacaaaat
    75361 tttccttttt atatttaaca ttgtatcaca aatataaaaa ggaaaaaact ttttaaaaag
    75421 ggtatacact attcattttt tatcttataa ataagtcttt atcactgttt tatatatttt
    75481 aaaatagaca caagacctat ttctaaatct tttataaata ttttaggtga aatttctttt
    75541 taaacatatc taaaaagtat ttgtagtaat attaatggac tgaatttctt aaagtatata
    75601 tttctttaca tgttggagtt tgtgaagttt gagaattgga gggtgaaaga aacatgtaat
    75661 ggattaatat taaagaactg tatttttata atatttgtct taaaaataaa aaaatctatt
    75721 gacttgaagt taactccaag tagtaaagta taattataca agaattatat acttgtaaga
    75781 atagttaaag tagagagaaa aaacaaacaa gttttaaagg gggaagtaaa acaatggagg
    75841 caaaaaaatt aggatttgga ttaatgcgtt taccaataaa agatgaaaat gatgttacaa
    75901 ctattgatat ggaacattta aataaaatgg tagacacttt tttagaaagg ggatttacat
    75961 attttgatac ggcctatgtg tatcacatgg gaaaaagtga gatagcactt agagaatctc
    76021 tagtaaaaag acacaaaaga gagagtttta ctatagctac aaaacttcca ttaatggctt
    76081 taaagaaaaa agaagagcaa gaaaccattt ttaatgagca attagaaaaa tgtggtgtag
    76141 actattttga ctactatctt ttacacaata taggcgtgtc acattatgaa gtagctaaaa
    76201 aatttgatag ctttaagttt attgaagaaa agaaaaaaga aggaaaaata aagaatattg
    76261 gtttttcttt tcatgatagt gcagaattat tggataaagt cttgagtgag catccagaag
    76321 tagacttcgt gcaattacag attaattatt tagattggga taatgaaagc atacaatcta
    76381 gaaaatgcta cgaggtggca gagaagcata ataagcctgt aattgttatg gagccagtaa
    76441 aaggaggtac acttgcaaag ataccagaaa aagcagagaa attattgaat gactacaatc
    76501 cagatatgtc aatttcatct tgggcaatac gttttgcagc aagtaaagat aatgttatga
    76561 tggtcttaag tgggatgtca aatatggaac aattattaga caatacagga tatatgcaaa
    76621 atttcaaacc attcgtacaa gaggaatata atattataga tgaagcagta gagattatca
    76681 acgaatcaat tttaattcct tgtactgcct gtcagtattg tgtagaaggc tgtccaaaaa
    76741 atatcgctat tccaaactat tttgcgttgt acaatgcaga aaaacaagct cttaatactg
    76801 gattttcaac acagatggtc tactataata attatactaa aacttatgga aaggcctcag
    76861 attgtattga atgtaaacaa tgtgaagaaa gctgtcctca acacattaaa attatagatg
    76921 ctttaaagaa tgtagcagaa acctttgaga aataattcaa tctatacaat atcaagagga
    76981 gaagatatgt atgacgattt cagaagttag taaaaaatac gagctttcag cggatacttt
    77041 acgatattat gaacgaattg gtttaatacc acctgtaaat agaaataaaa gtggaattag
    77101 gtcttttaca gaagaagatt gtgagtgggt taatttcata aagtgtatga gaggtgcagg
    77161 tctttctatt gagacattaa ttgagtatgt agctatgttt caacaaggga gcagtactat
    77221 caaagctaga aaagaacttt taatagagca gagaaaccaa ctagcaaaaa gaatagaaga
    77281 aatgcaaaaa actttggaac gattaaattt taagattgat agatatgaag aaggtattat
    77341 tgagaaagaa aaagtattaa agagcagtag aaataagaaa atggaggcag tatcttatga
    77401 gtaaaaatgt attaatagta tcagctagtc ctagaaaagg tggaaattct gatttattgt
    77461 gtgaccagtt tatacaaggg gtattagaat ctaaaaatca agtggagaag atatttttaa
    77521 aagataaaga tattaactat tgtactggat gtggtgcttg ttatgaaaaa gaagcaaatt
    77581 gttcacagac agatgacatg ggagaaattc ttgaaaaaat gattggggca gatgtgattg
    77641 ttttagcgac tccagtttat ttttatacta tgaatgcgca aatgaaaact atgattgata
    77701 gaacttgctc tagatatact gaaatttgtg ataaagattt ctattttatc gttacagcag
    77761 cagatgaaaa tgtggctgat atggaaagaa caatagaagg atttagagga tttacatcat
    77821 gtctaaatgg ttctaaggaa aaaggtatag tatatggtgt aggtgcttgg aatgtaggtg
    77881 atattaaggg aagtaaggca atgaaacagg catatgaaat gggtaagttg gcgtagtatg
    77941 tttgtataaa attgtataaa attgaatata ttcttatcat ttcaacaata aaaagaggct
    78001 tatctgtatt ttgagacagc ctctttgtgc tatatcattt atttttattc tatatcctta
    78061 ttataccctt tattttttta tgcctattta agtatctgtt gttactcata tattttaata
    78121 attaccctta aaaaactata cagattcctg cataatatta ttttctttag taattaattt
    78181 ttctaggttt ttccaatcca tttcttccat atttgcaaaa taatgaccac ttcctatgca
    78241 agtcaggtaa gactttccat ctaattcaaa cgaagaacct ccaatacact ttgaattaca
    78301 tttcttacaa gtacgagttt tagttatact atctttaata gttttagagc attcctccac
    78361 agcatcttta tatgtattta gatgaaaaag atttgcttta actctccatt ttttttcatt
    78421 acattcaata gtaaataagc tacgttttgg tttcttagtt gaatatacta ttttgtaaga
    78481 acgagcacct gtttttggtt caattttaca atctaatgat ttaccaagtt catgtaaatg
    78541 acatgcaata tcataatctt taccacttag tacttctaac atttcttctg ttgtttctgc
    78601 tgttaattca cttttgctca tattaaaaaa atccccctat ataaataata atttcatatg
    78661 aaaataatta atataagtat attaaataaa actattaaaa tcaataaaat tactgcaatc
    78721 ttacattatt gggcatagaa tgagagctaa tgatttatta gaaataagac ttaatagtat
    78781 gaaaatttac ttagacgtgg tacaattcta taaaagattt aaaactagga aaggagaaag
    78841 ttgttgagat acggccaata taacaattta tttataagtt gtaaatttat tatgagatta
    78901 attagattgt taagtaatac tatcaaaagg tagttttttg gattttcagc aacagtggta
    78961 gatattgata attttttggt tattattata tttagctgtt atattttcaa taggctctat
    79021 gataactagg gtaaataagg tatgggtaaa ggttttatta tttataatag tgggattgtc
    79081 aactatagtt ctttcatatg taataacttg attatgcaaa attctatgaa gcaggaaccc
    79141 tgtgacttta gtcatgggag gttcagaatt taaggttaaa aaataacttt aatctgtaaa
    79201 ttctcaaaac aagaattctc atgttggtaa atttctagat atgagaaaaa aattgacatc
    79261 ataaaaaagt aattttagag cgttttttta cgaaaaaatt cagaagtaac tattttgaat
    79321 gacttcacaa aattttatat ataaataaag tttggcacgt taattgcttt aataaaagtt
    79381 aacaagacac aaataaacat aatcatctaa gtattgatta tttgaagttt tagatttgaa
    79441 gtttaatcta aaggttcgta ttaatattgg tgtttaggaa attttatttt cctgtaaata
    79501 taacttaata ataggaggtg aagtcatgtc aaaagtagta atagctacag agaaagctcc
    79561 aggagcaatt ggaccatatt ctcaagcaat tcaagttgga aacatagttt ctacatcagg
    79621 tcaacttcca atagatatga agactggcga gttagtatct gacccaaaag aagcaacaaa
    79681 agcttcttta gataatgtaa aagcaatatt agaacaagct ggtacaagta tggataaaat
    79741 atttaagact acagtattcg taaaagattt aaaggatttt aatgatatta atgaagtata
    79801 tgctacatat tttaaagaaa atccaccagc aaggtcatgt gtacaagttg ctcaattacc
    79861 aaaagatgca gttttagaaa ttgaagcaat ggcagtatta taagtattat aacaataaaa
    79921 ccaataacaa aactaataaa aattaaaaac taataaaaaa agtctacaat taaaaaagtt
    79981 tatattaaaa aagtctatat aaaaatctat ctaaatattt aactattgag cttttctcat
    80041 ataatgtata tttttatgtc aaaaatatat ttgttatatg aggaaagctt ttttttatta
    80101 ttgtattgtg atttatttta ttgtctgcat attataatta aggttactat ataaaacaaa
    80161 ctttttataa attaatattg tctgttttaa cgcaaatata taaataagta atgttttata
    80221 tatattttaa agagtgacta tatgttaaaa tatagctaaa ctgataattt ggtaaattaa
    80281 attctaatgg gggaaactat gttaagtaaa ttaaaagaat ttcaacaaga aatgataaaa
    80341 tacactgaaa cagtagctag tgtgttagat gtagatattg aaatagttga cgatagatta
    80401 ataagaatta gtggtacagg tctgtataaa tcaaagataa atgaaagcgt tgttactgag
    80461 ggctttattt atgacaatgt tatacagaca ggtcaggaat tagtcgtatt ggatatatgt
    80521 gataatcagc tttgtataga atgttcccat tacatgaaat gcctaaataa agtaataata
    80581 gcagttccaa taaagtataa taatcgaact ataggagtaa ttggtgctat tagcacagat
    80641 aaaactaaaa aagtagaaat aagtgcaaaa atagataatt atttaaaatt tgtcaatcat
    80701 atatgcgatt taatttctat gaaaatagaa gaacatgaag cgtcaaaaaa tagcagtaga
    80761 aaaatggata tgatgataga aatcattgaa aatgttgaaa aaggcgttat aatactagac
    80821 ataaatagca aaatatctta tataaacaat atagctctaa aaaaattaga tatagataaa
    80881 aatataatag aaaatatagt aaatatagta tctgttgaat cttcctcaaa tggacatgaa
    80941 ttactagaaa ttgatattga taacaaaatt tataatataa atgccaaaat aattccggta
    81001 tatccatata taaatcaata cgacaaaata ataatatttg acaagacata tatcaatcat
    81061 aaaggacatg gaaaagtgaa tagtggctgg ggaaatagtg atatagagtc tataattgga
    81121 aattcagaag caatgttaaa agtaaaagag agaactaaga aattagcaaa gtcaaattca
    81181 actgtactta taactgggga aagtggtacg ggtaaagagc taattgctag ggctatacat
    81241 gctgaaggta gcagatggaa caagccattt attgcgataa actgtgctgc tattccagaa
    81301 aatctccttg aaagtgagct atttggctat ataaaaggtg catttagtgg agctagtagt
    81361 ggagggaaag taggtaaatt tgaacttgca aatgaaggtg ttatatttct tgatgaaata
    81421 ggagatttgt caatgccttt acaagcaaag ttacttagag ttttacaaga gagaaaattt
    81481 gcgagaattg ggtcaaataa acttatagat ttagatataa gagtgattgc tgctacaaat
    81541 aaaaacttat tgaaattagt aaatgaaggt aagtttaggg atgatttata ctatagattg
    81601 aatgtaattc ctataaattt gccacctctt agggaaagaa aggatgatat tgaggcaata
    81661 atgatgaagt ttgcttctaa atattcttta gaattaggta ttcagttaaa taaaatagaa
    81721 gaaaatgtaa tgaatatgtt gattaactat aattggccgg gaaatataag ggaactggaa
    81781 aatgcagttg aatatatgat gaatttggtt ggagatgatg gaataatata taaagatatg
    81841 cttccattag atattttaaa ctattataat attaatggaa atatttataa aaataaggat
    81901 gtaaatataa tatttgaaga tgatatagtt ggagggatag tagagaatca agaaaggata
    81961 ctttcaatta aagaattaga gttgacttac ataaacaagt tgttgaataa gtatggaaga
    82021 gatacaaaga cgaagaagaa gatagcaaaa gacttaggca taggtttggc tacactatat
    82081 agaaaattgg aagaggaaca gtagtaatat aatattaaaa tgataggtat ttgtgtgttt
    82141 ttatagatag gtatttttac tttatggtgg aggtgttatt atgcaatatg cgatagagct
    82201 atattatgat aaaaaaacgg aaaagcaact ttttgattta tctaaaaaaa tcgctgatga
    82261 aaaaattagt acgaagtact tagaatggaa aacaagacct catcttactt tggcttgttt
    82321 caatgatgtg gatgaggctt gttgcataga caagctaaag gggtttgcac aaaaccataa
    82381 ggtaatacct gcttacattg gttcagtagg catgtttaat gatactaaaa cggtgtttgt
    82441 atcacctata atgaatagca atgtatcagt ttcagagaga attatatgag gtactacaag
    82501 attttgattc aaatggttgg gaatggtacc accccaatcg ttgggtaccc cattgcacaa
    82561 tagcacttac tggagatgat gaaagtgagg ctttttttcg ggcaagtgat gtattactgc
    82621 gtgagttcaa aaaaataagt ggtgagtttg tctcaatagg attggttaaa attacatttc
    82681 ctgtggaaga aatttttaca gtgaatatga ataggtaact ttaactctgt tgtggatgtt
    82741 atgaatattt tgtatagtgc atttcatcag ataaatgcga agttgcgaat ttcgacagct
    82801 caatagtcgc aagtagggca caatggtaca catattgcac ttttgcttat tggggggacg
    82861 accatgtaaa cttctgtaag aagctatgaa aagatttaag ccattttgga ggctttgggt
    82921 aataaagaat atttgggtaa taaagtatat aaatttatag agtttatgcg taagaaggtt
    82981 atagccatat atttatagaa aataaaagta gtaatatatc tgacaaaaca atagacatat
    83041 aaaaaataag agcataataa aggaagaatt aagataaata aacgatttct cgaattgata
    83101 attctcgtat tgataaaaat ttcccaatat gagaattttt tcttttataa atcttatttt
    83161 tcacactcaa aatttataaa ctttaaagaa attttataaa agtatgtaat tccaacataa
    83221 aaaaattgtt tttagaataa ataatattgg cacggtaatt gctttgctat aaggcacaaa
    83281 gataaaaaat taaaatgaca ccgaaaaatt tgaaagggga atataaaata aatgagagaa
    83341 ataaaatgga aattaaataa cttacctaaa tcagaggaaa aagaaaaagg aattgaattt
    83401 ttaaaagatg aagagattgc aaaggctaaa gcttttcatg aaagctttcc acaatatgaa
    83461 aagactcctt tagttaaatt agataactta gcaaaatcac taggcgtaag cggaatatat
    83521 gttaaagacg aatcttatag atttggctta aacgcattta aagttttagg tggttcttac
    83581 tctatgggta gatacttagc tcaaagatta aatacagata tatctgagtt agggtatgac
    83641 aaattgactt cgaaagaaat aaaagaaaaa ttaggagaaa taactttctt tacagctaca
    83701 gatggaaatc acggtagagg agttgcttgg acagctaata aattaggtca aaaatctgtt
    83761 gtattaatgc caaaaggttc ttctgaattt agattaaata aaatcaaagg agaaggtgca
    83821 gatgctagta taacagactt aaactatgat gatgcagtaa gattagctaa tgattatgca
    83881 gaagctgatg accatggagt aatggttcaa gatacagcat gggacggata tgaagaaata
    83941 ccagcatgga taatgcaagg atatggaact atggctaaag aagctataga acaattaaaa
    84001 gaatatggag tagatagacc aacacacgta tttgttcaag caggagttgg ttcacttgct
    84061 ggtgcagttc aagggtatgt tgcttcaata tatgatgaat gcccaataac agtagtagta
    84121 gaggctgatg aggctgattg ttactataaa tcagcagaag ctggagatgg aaaaccaaga
    84181 tttgttggag gagatatgcc aactataatg gcagggcttg cttgtggaga accaaacact
    84241 ataggttttg aagtgttaaa aaattattca tcagcattcg tttcagcacc agactgggta
    84301 tctgctaaag gtatgagaat attaggaaac cctctaagag gagatgagaa agtaatctct
    84361 ggagaatctg gagcagttac aactggagca ttagtttcaa tccttgaaag tgaagattta
    84421 aaagacttaa gagaagcttt gaaactagat gagaactcta aagtattatt aataagtaca
    84481 gaaggagata ctgaccctga taaatataga gatatagttt ggaatggtga atgccaaagt
    84541 aaataaaaga cttaaggaaa ataaaaactt aacaaataaa tgaaaaattt aaggaaaata
    84601 aaaacttaaa taagtgaaaa ctttaaataa aataaaaact aaaacaaatg aaaaatctaa
    84661 ataaatgaaa aagtaaagca aatgaaaaat tcaaaataaa aactaaataa aattaaataa
    84721 aaccaaataa aattaaaata aagtctaaat aaaaatttaa taaataaaat ataaagaaat
    84781 aaaaaatcaa tagattaagt gaaatgctaa taaattaaaa aagtgaaata tcaaaataaa
    84841 acgtcaacaa actaaaaatt atatggggga atatcaaaat gttaaatgaa acaagaaaaa
    84901 aacaattaac tgaagtatgc caagacttaa taagaaatgc tagttattca ggtcaagaag
    84961 aaaacgtagt aaaagctata gaagaaaatt ttgctttact aggttttgat agttggtcaa
    85021 gagatagata tggaaatata gtaggatgta taaaaggaaa taaaccaggt aaaaaaatat
    85081 tatttgacgg gcatatagat acagttccag ttccagatgc aagtaaatgg agtgttcctc
    85141 cttttgaagc gagaatagta gatgggaaaa tatatggtag aggaacttct gatatgaagg
    85201 ggcaagtaag tgctatgatg gctgctgttt catactttgc agaagatact aacaaagact
    85261 ttgaaggtga attgtatgta gcaggtgttg ttcatgaaga aatatttgaa ggtgtttcag
    85321 ctagagagat aagtaaggct gttcaacctg attatgttgt aataggtgaa tcttcagaat
    85381 taaacttaaa gataggtcaa agaggtagag gagaaatagt agtagaaact tttggtaaac
    85441 cagctcactc tgctaatcca gaaaaaggta taaatgcagt ttataaaatg gctaatgtaa
    85501 tccaaaagat acaacaatta gtacctccaa ctcatccagt tttaggtgat ggaatattag
    85561 ttttaacaga tataaaatct tctccatatc caggagcatc agtagtgcca gattattgca
    85621 aagctacttt tgatagaaga ttattagttg gagaaacaag agaaggcgta ttagctccaa
    85681 tacaagcttt attagatgaa atgatgaaag aagacccaga attaaatgca aaagtttctt
    85741 atgcagttga aaaagctgat tgctatacag gaaatactat agaaagtgaa agattcttcc
    85801 caggttggtt atatgatgaa gaagatgaat ttgttcaagc agcgtataag ggattaaaag
    85861 aagctggaat agattcagaa ataactcaat actcattctg tacgaatgga agtcactacg
    85921 caggtgaggc tggaataaga actataggat ttggtccttc taaggaaaat cttgctcata
    85981 caatagatga gtatatagaa ttagaccaat tattcaaagg tgcagaagga tattatggta
    86041 tattaaaatc agtttataaa aaataattag gagatggttt tgtgagaact ttaataaaac
    86101 atgggcttat agtagatgga aataaaacac cagcatatga aggagatatt ttaatagaaa
    86161 acgagaaaat attaaaaata tcacaagatt taaacgaaga agctgacaaa gttatagatg
    86221 caaaaggtag agttatatgt ccaggtttca tagatactca tagccattca gacctagtta
    86281 ttttagttaa tccatataat gaggtaaaga ttagacaggg aattacaaca gaagtgttgg
    86341 gccaagatgg tatatctatg gcccctctac ctcaagagca tatatcttct tggagaaaaa
    86401 atttagcagg tctagatggt gaaagtgatg aaattgattg gaaatatgaa acaacagaga
    86461 attatttaaa aatgatggat tacaatgggg taggtttaaa tgaaacttat ttagtacctc
    86521 atggaaacgt tagaatggaa gctatgggac ttgaagatag acctgctact aaagaagaaa
    86581 ttcaaaaaat gtgtgaaatc acagagagag aattaaaagc aggagctata ggattgtcta
    86641 ctggacttat atatatacct tgtgcatact cattgactga agaaattata gagatgtgta
    86701 aggtagtagc aaaatatgac ggagtatttg tagttcacca aagaagtgaa gcagatacaa
    86761 ttttaacttc aatggaagaa atcatagaaa ttggaaaaca atcaggagta aaagttcatt
    86821 tttcacattt taaggtttgt ggtaaagcaa actggaagta tataccacaa gttatagagc
    86881 ttttagaaaa agcagaaaag gaaggaataa gagtatcttt tgaccaatat ccatatgcag
    86941 caggaagtac aatgcttgga gtagttttac ctccttgggc acattctgga ggaactgata
    87001 agttaataga aagattaagt gatgaaaacg aaagagcaaa aatgaaaaaa gatatagcaa
    87061 atggaataga aggctgggat aattttatag aatttgctgg aatagaccaa atatttgtaa
    87121 caagcgtaaa aacagaaaag aataaagata caattggtaa gagcttatta gaaattggaa
    87181 aaatgagagg aaaagaccca ttagatgcaa cgtttgattt attaaaagaa gaagaaaatg
    87241 cagttgggat ggtagatttc tatggattag aagaacatat aataggattc atgaaaagag
    87301 acgagcaaaa tgtgtgtaca gatggtcttt tagcaggtaa accacatcca agagcttatg
    87361 gttctttccc taaaatacta ggaagatatg ttagagagtt gaatgtgtta acaatcgaag
    87421 aagctgttta taaaatgact aaaaaggcag ctacaagctt tagtataaaa gatagaggcg
    87481 aattaaaaga aggatatttt gcagatatag ttatatttga caaggataca gttagtggtt
    87541 gtgatgattt tatcaactct atgcagtacc ctacaggtat agattacgta attataaatg
    87601 gaaattgtgt tgttgaagaa ggaaaataca ataaaattaa agcaggaaaa gtacttaaaa
    87661 attaaatctt agctttaagg aggaataaat ttatgatttt agtggggaat gggaggctaa
    87721 taagccaaga ttctttaaat ccctatatgg aagatggatg tgtagtaata aatgataatc
    87781 taattgaaga tataggtact acagaagaaa tgaaagctaa atatccagta catgaattta
    87841 tagatgcagg caagaaaata atcatgccgg gtctaataaa ttcacatatg catatataca
    87901 gctcatttgc tagaggaatg gcagttccag gaaaaccttc tgagaacttt ttagagatac
    87961 taaataattt atggtggaga cttgacaaac agcttacact ggaagataca aaatacagtg
    88021 catatgcaac gtatatagaa tgtataaaaa atggtgtaac aacagtttat gaccatcatg
    88081 caagtccaaa tgcaattgaa ggcagtttgt ttacaatatc agatgttgca aaggatttgg
    88141 gaattagaac gtgtctttgt tatgaagtgt ctgatagaga tggtggaaat acaatccaac
    88201 aaggtatcaa tgaaaacgta aactttataa aatacgctca aaaagatgat acagacatga
    88261 taaaaggaat gtttgggctt catgctgcat ttacactatc tgatgataca ttaaataaat
    88321 gtgcttcaga gatggctggt cttgatacag gttaccatgt acatgttgct gaaggactag
    88381 cagacttaca atataatgta gataaatatg gaaaaagggt tttagaaaga ttcaatgatt
    88441 ttggagtact aaaagaaaaa acaatagcag ttcattgtat atatataagt gcagaagaaa
    88501 tcagtctttt aaataaaaca aatacatttg tagttcataa tccagaatct aatatgggaa
    88561 atgcagttgg atgttctcct gcaatagagt tattaaaaag gggagttaca gtaggattag
    88621 gaactgatgg atatactagc gatatgtttg aatcattaaa agttgccaat ataattcaca
    88681 aacatcactt aaaagaccca agagttggat ttgaagaatc acaaacaatg ttatttgata
    88741 acaatagaaa aatagctaaa gcttattata aaaatgacct aggagtactt aaaaaaggtg
    88801 cttatgcaga tgttatagta gttgattata taccacatac tccattgaca gaaaatacaa
    88861 tagggggaca cactattttt ggaatgagtg gaagaagcgt agatacaact attataaatg
    88921 gaaaggtact tatgaaagat agaaagttaa taaacataga tgaagaaagt attttagcaa
    88981 aaagtagaga attaagtcaa aatctatgga atagaattta aataattgat attaatatac
    89041 aagtaagaaa caagaattta gatgttaaaa acagagctta aacaaaaata ggaatttaaa
    89101 tgaagtatat aaataaaaaa tatacggaat agaatattaa attaaatata gaagggagac
    89161 ccaaaatgtc tcgagataaa agccctaaaa tagctccagt agatgaaagg ttacctgtat
    89221 ccaaatcttg ggtattttca ctacagcacg ttttagcaat gtgtgcagga gcagtagcag
    89281 ttcctatgat gataggaagt gctgcacgac tagatcatgc tagtattgtt tttttaataa
    89341 atgcttgctt gtttatggca ggtataggaa cactcataca aagtgttggt ataggaaata
    89401 ttgtaggtgc taaaatacca gtaatagaag gaactagttt tgccgctgtg gcaggtatga
    89461 cagcaatagc ctcatcatat agtgattcac aagttgcaat acagacagta tttggctctg
    89521 taatagtagc tggattgttt tgtatattac ttgctccagt atttgaaaaa ttaataagat
    89581 ttttccctag tgtggttact ggaacagttg ttatggtaat aggtatttca ctatttccag
    89641 ttgggataaa atggataact gacaacaaat tggaagcggt tgaaccaaac tcattagcat
    89701 tagctttagc agtattggca ataactttac tttcatttaa attcttaaaa ggaatttgga
    89761 atagtacagc tatattggta ggtatagtac ttggtacact actttccatg gcagttggaa
    89821 tggcagattt tagtgcagta aatggagttg gttggcttaa cttaaatgta cctttaaaat
    89881 ttggaatgcc tagatttgaa ccatctgcaa tactatcatt gtgtttgatt atgttggtac
    89941 ttatgacgga atcagttggt aatatgatag ctattcatga aatggtagac aaagaagtaa
    90001 caggtaaaaa tattaaaaga ggtctatttg gagatggaat atctacagca ttatctggaa
    90061 tattcaattc atttccaata actccatttg gtcaaaatac aggaatagta ggtcttacag
    90121 gtataaaatc aagatttata gcagtttacg caggggttat attgctagta cttggattct
    90181 taccaaaatt tgcagcaata ataggagcaa tacctaagcc tgttcttgga ggcgtaggat
    90241 ttgcaatgtt tggaatggta atggttggag gagtaaaaac tttaagcaaa gtgaaatttg
    90301 atggaactaa aaatggggtt atagtagcag ttagtgttgg tttgtctatg ataccactaa
    90361 caaatcctga tttctatcat aatttcccaa cttgggtaca aacaatattc catagtggaa
    90421 taacaacagg tagtttatca gcagtgcttt taaacatatt cttcaatgaa ctaggttcta
    90481 aaaaagaaaa aaataataaa gttgaagaag caaaataaaa ataaaacaag taaaaatcta
    90541 tattgactta aagtcattaa gcattaataa aaataattca gagatattaa taattcaaca
    90601 aaactatctt atattttaaa taatataaaa aagtaaataa ataacgaata caaataaaag
    90661 aatacaatct aaatattaaa aatatttaat ttaagggaga gataataata tggctaattt
    90721 aaaagttaat ttatatggaa tggaattttt aaacccaatt atgacagcgg caggacctgg
    90781 agcaaaagat ggtgctctct gtcaagaggc agcaaaaggt ggagcaggag ctatatgtac
    90841 aaaaactata tctgtattac cagctgatgt accaagacca tgtatggcaa aaactaatag
    90901 tggtttttta aacacagaat tatggtcaga gcttccaaaa gagcaatgga tagaggaaga
    90961 atacaaaaag gtaaaagaaa cagggcttcc agtaatagta agtatgggat attcagcaga
    91021 gcaaatagct gaggttgctc ctttagtaaa gccttatgca gatgcagttg aattatctac
    91081 tcattatgta ggtacagacg ttgcaccaat agtaaatgct ttaaaagctg caaaagctgt
    91141 tttagatgta ccagtattta tgaaaatgag tcctcatact gacattcaaa ctattgcaaa
    91201 agctgttgaa gaagcaggtg cagatggact tgtaatgata aactcatttg gaccatgtat
    91261 ggcaatagat gtaaatacag gataccctat aatgggaagt aaagctggat acggatggtt
    91321 atcaggtgca ccaataagac ctctagcaat aagatgtata tatgaagctt ctcaagttgt
    91381 aaatatacca ataatcggtg ttggtggaat cacttgtgga aaagacgtgg ctgaaatgat
    91441 gatggctgga gcaagtgctg ttcaagtatg tacagaagca atccttaaag ggccttctat
    91501 ctatggaaaa attgctaaag aattaaatga atttttagat gagaatggat atgctgatgt
    91561 taatgaaata aaaggattaa ctcataagaa aatggcagct agagggttta gaactcattc
    91621 agtagctcca gtagtggatg ataacaagtg tatcaaatgt aagcaatgtg taacaagctg
    91681 tgtatacgaa gctttagaag ttacagataa gttgaacata gatgcagaca agtgttttgg
    91741 atgtggatta tgtgtaacta gatgtccaaa aggagcttta agcatctgct acaatcaagt
    91801 agaagctatg aatgagtaat aattatatag aattttggta attaacttag agtaataggt
    91861 attaggataa taaaatatat cagataaaca aacacgcatt atgcattaat aatacagtct
    91921 aaagttaata attttattat attgatttaa ctaaatatta gtttagctga atattaattt
    91981 aattaggtat tggtttaatc ttaatataga tttactacgt ataagtgaac taactagatt
    92041 actttaagtt aattttttca tataaaataa attgatacat tacaagtaag gtggagtcta
    92101 tatgggaatt gttttaaaag gtgggaaagt agtaacaagt gaaaaaactt actgtgcaga
    92161 tgtaagaata gaagatgaaa aaatagtcga tataggtctt gatatatcaa aagaaaatga
    92221 tgaaatagtt gatgttagtg attcttatgt tataccagga ggtatagata cacacacaca
    92281 ctttgactta gatacaggcg ttactaagac agcagataac tttgagacag gaacaaaagc
    92341 tgctatagta ggaggaacta ctactatatt ggattttgct acacaagcta aaggatgttc
    92401 tttgaaagac ggattagaag aatggcatga aaaagctaag gataaatcat attcagatta
    92461 tggctttcat atggctataa cagattggaa tgactcggtt tgtaatgaaa tggaggatat
    92521 ggtgaaagag ggagtatctt catttaaact gtatatggcg tataaaggaa gccttcaagt
    92581 tgatgatggt gtaatctttg aagcattgag aaaagcagaa gagataggag ggattattgg
    92641 tttccattgt gaaaatgggg acataatatg tgaacttgtg gataaagcaa agtcagaaaa
    92701 taatttatct ccaaaatatc atcaattaac tagaccagct ataatggagg cagaagctac
    92761 atcaagactt atgaagatag ctgaagttgc aaactctaaa gtatatgtag ttcacttaag
    92821 ctgtaaagaa tccttagagg aggttttaaa agctaagcaa agaggtgtgg atgtaatagt
    92881 tgaaacttgt cctcaatacc ttctattaaa tgatgaatta tatgatttag atggatttga
    92941 aagtgctaag tatgtaatgt caccaccttt aagaaataaa gaaaataatg aaatattatg
    93001 gaatgctatt ttagataatc aaatagacac tataggaaca gaccattgtt cttttaatta
    93061 taagggacag aaggaacttg ggataaacga tttcagtaag ataccaaatg gttctccagg
    93121 tgtagaacat aggatgagtc ttttatacac atatggagtc tgtaaaaata aaatatctat
    93181 caacaagatg gtagaagtaa catcaactaa tgcagcaaag ttgtttggaa tgtatcctaa
    93241 taaaggaact atagaaatag gcagtgatgc agatatatta gtcttaaatc caaacaaaaa
    93301 atccaaaata gaagcaaaaa aacaattaca aaatgtagat tatacacctt atgaagggta
    93361 tgaagtggat tgtcaaatag aacatgttta tttaagagga aaagaagtag ttagagatgg
    93421 agaattaatt tgtaatactc caagtgggag atatataaat aggcgtctaa caaagggggc
    93481 tagagaatgg tagaatttat attaaatgga gagaaagttg ttgcaaaaga aggcaaaaaa
    93541 cttttagact tccttagaga agatatgaat ataacttcag ttaaaaatgg atgttcagaa
    93601 ggtgcttgtg gaacatgtat ggttataata gatggtaaag ctactaaagc atgtgtattt
    93661 aaaactgata aattagctgg gaaaaacata acaactatag agggattatc agagagagaa
    93721 agagatgttt ttgcatattc ttttgctagt caaggagctg tacaatgtgg attttgtatt
    93781 ccaggtatgg taattagttc aaaagcactt atagacgtta ataataatcc tactgaagat
    93841 gatataaaga aagctttaaa aggtaatata tgtagatgta cagggtatgt aaaaataatt
    93901 aaagctgtcg atttagcagc aaagattttt agagaaaata tagatgtgcc aaaagtaaac
    93961 tgtaaaggtc tagtaggtga aaatcttcct agagttgatg cagttggcaa agcacttgga
    94021 tattctgaat atgtagatga tatgagaatt gaaggtatga tttatggttc agcagttaga
    94081 gctaagtatc ctagagcttt agttaaaaaa atagatatag aagaagcaaa agcttatcca
    94141 ggagttgttg cagttctaac agctaaagat attccaggaa ctataaaggt agggcactta
    94201 aagaaggatt gggatacttt aataccagaa ggtgaaataa ctagatactt aggagatgga
    94261 atagttttag tagcagcaga aactagagaa gcgttagaag aggcaaaaaa gctagttaag
    94321 atagattatg aggaattaac accattaagt actacagaag atgcgcttag agaagatgct
    94381 ccaaagatac acgagacagg taatatactt gttaaggaac atttagtaag aggaaacgct
    94441 aaagaaaaga tagaaaattc aaaatatgtt gtaactaaaa gatatactac acctgctaca
    94501 gaacacgcat ttttagagcc tgaatgtgca gtagctggtc cttatgaaga ggatggagta
    94561 ataatctatt caacagacca aggggtgttt gccactcaac atgaatgtgc agatatgtta
    94621 gggctaccat atgagaaggt aagagttata aacaaaatgg taggaggagg atttggtgga
    94681 aaagaagaca tgagtgttca acaccatgca gccttacttg cataccatac taagagattt
    94741 gtaaaagtac agttaacaag aaaagaaagt attattgttc atccaaaacg acatgccaca
    94801 gaaatggaat ttacgacagc atgtgatgaa aatggatact taacaggtat gaaggcaacg
    94861 ataatatctg acacaggtgc atatgcctct cttggaggac cagtgctaca aagactgtgt
    94921 actcatgcag caggaccata taattatcaa gatattgata taacaggaac agcagtatat
    94981 actaataacc ctcctggagg agcatttaga ggatttggag ttacacagag ttgttttgca
    95041 actgaatgta atctaaattt acttgctgaa atggttggaa tatctccttg ggagataaga
    95101 tataaaaatg caataagacc tgggcaagtt ttaccaaatg gtcaatttgc agatgaagga
    95161 acagcaatgg cagagacact agaagctgta aaagaagagt atgaaaagca tccacgtgca
    95221 ggtatagctt gtgctatgaa aaatagtggg gttggagttg gtatacctga tgttggaaga
    95281 tgtagattgg tgataaaaga tggcaaagtc cacataagaa caagcgcttc ttgtataggt
    95341 caagggcttg gtactgttat gactcaagta gtttgtgaaa ctgctagcct acttcctgaa
    95401 aatgtagtgc atgaagttcc agatactcat ttaacaccag actctggtaa tactacggct
    95461 tcaagacaga cagtatttac gggagaagct actaaggttg cgtcaaataa gttaaaagaa
    95521 gctttagact ctggcaaaac tttacttgat ttagagggag aagagtttta tggagaatat
    95581 gaaagtatta cagataaaat gggttgtgac aaagaaaatc cagtaagtca tgttgcttat
    95641 ggatatgcaa cacaagttgt aattctggat gaaaatggaa aattggaaaa agtagtagca
    95701 gcacatgatg ttggaagagc tataaatcca atcagtttag agggtcaaat agaaggtgga
    95761 gtagttatgg gtcttggata tgctctaacc gaagattacc cagtagttaa ctctgttcct
    95821 acagctaagt ttggtacatt aggtttattt aaatctacag atataccaga aatacaagct
    95881 ttaatagttg aaaagaacaa agatttattg gcttatgggg caaaaggtgt aggagaaatt
    95941 tgtacagttc ctactgcacc agcagtacaa aatgcctatt atgtatttga taaagaattt
    96001 agaactgaac taccactaca aaatactcca tatagtaaaa aaaagtaaca aaaaaaagaa
    96061 atattaaata aaaaaataga tgaaattgta aataaaaaaa tagatgaaat tgtaaataaa
    96121 aaaaaccctt ttgcataagg tatattttaa atgatagaat atacatatac aggagggttt
    96181 ttcttttata aaataagttt aataaaattg agaggtgagc agaatgaatt ttaataatag
    96241 tctagaatta actcagtctc agaagctaat aatgactaca caacttaagc aatcattatc
    96301 aatattaaat atgagtaagt tagagttaga agaagagata aaaagagaag cagagaataa
    96361 cccattagtt gaagtcgaga aaagtgggga gataaattgg gaagagtata taaaagacat
    96421 ggataggtca agacgtttag atagaacaga gataagctat aatcctgata atgaagttaa
    96481 tctagaaaat ttagttaaat attcgtcaaa tttatatgaa gatttaaaat ttcagataag
    96541 cctgtataaa cttacagaca aagaattgga agtctgtgag tatataattg atagtttaga
    96601 tgaggatgga tatttaagaa ctgaggaaaa agtaattgtt gataccttaa agatagatga
    96661 agaacttttt gaaaaatgtt taattagtgt tcagcagttg gaacctagtg gagtaggggc
    96721 aagaagtctt tcagaatgtc tgatgataca aatggctagt ttaggaatat ataatgaaat
    96781 tttagaggaa atcgttacaa aagacttaaa tttaattgca aataataaat acaaagagat
    96841 aagtaaaaag tacaatatgt cattacaaaa atgtgttgaa ctaataaaca taattaagac
    96901 attagaccca aaaccaggaa gaacttgttc agtagagaaa agtgtataca ttcaacctga
    96961 tgtaacagta gaaaaaattg atgatgaatt tattgtatat ttaaatgaaa aagattctta
    97021 tcaaataaga atcaatagtt attataaaga catattaaaa aattcacagt ctgatgaaag
    97081 tgcaaaagaa tttataaaag aaagactaaa ttcagcaaca ggacttatga aaaatataga
    97141 gagtaggaaa actacagtac ttaagattgc agaagagata gtaaaagctc aagatgaatt
    97201 tttgagaaaa gggacaaaat atataaaacc tttgaaaatg aaagacatag cagaaaaatt
    97261 agaatttcat gaatctacta taagtagagg tgtaaatggt aaatatatgt taacaccttt
    97321 tggtgtatat gaatttagat actttttcag tagtgccatt gagactgaga ataatgaaat
    97381 ggcatctagt acaagcataa aaaaaattat aaaggaaaca ataaaagacg aaaataaaaa
    97441 gaaaccttta agtgatgacc atatatctaa gttactaaaa gaaaaaggaa tcaatgttgc
    97501 aagaagaaca gttgccaaat atcgtgaaga attaggaatc ccatcttcaa gtaagagaaa
    97561 agaatactaa taagtaaaag acaaatatca gtatggtaag gggaaaaggc taattaaaag
    97621 atgaatgtag attgggctaa aaattttatg aagtaaaata atagaatatg gaaaagtatt
    97681 aaaatgctat aagatattaa aaaactgggg aacattaaaa cattaagata gcaaagaaca
    97741 taaaaaacta aagaagatta aaacattaaa gaacattaga gctttgagga atattagaac
    97801 tttaaagaat attaaaattt ataaaatata aaaatactaa aaagaattga aaaactgggg
    97861 aacattaaaa tattgtaaaa tgctaaaaaa ctagaaaaca taattaaata agtgcttata
    97921 aagaatgatt aaattaaata agtgtttgta aagaataatt agatattaga aaaagaagaa
    97981 tacctagata aataaactat tattaaattt aataatcata aaaggaaaac acagggtata
    98041 aataaaaatc ttaactagat aaaagtaaac ttagttgtag ataaaaataa ctagaaataa
    98101 aagtaactag aaataaaatt atttatagta ataaatatta gaatgaaaat ataaaaaaat
    98161 atatataaaa agccttatat aaaaagaaaa aatactgtga tttgaaaatg gtggagaaat
    98221 attttataaa ataaaaaaat aaaaaagttt ttgaaaaact attgaaatct atacgcaaat
    98281 cctataaaat aaaaatatct tgtgggactt aaataaacac tagggacgaa aaaagtcccg
    98341 aaaggaaagc aataaggagt atgttatgaa gaacttatta gaaattcaaa agaaattgat
    98401 tccacaggca attgaactta tggaaagaag atactctatt ttaagacaaa tatctttaag
    98461 tgaaccaata ggtaggagaa cattatcaaa tgtattagag attagtgaaa gaataatcag
    98521 gtcagaaact gagtttttaa aagaacaagg acttatagac gtagctgtat ccgggatgac
    98581 tatcactaaa gaaggttctg atttgttaga taaacttaag gacatcatga gtgacatcat
    98641 gggattgtct aagttacaag atagagtcag agaaaagctt ggaataaaaa gggtaatatt
    98701 agttcctgga agctgtgatg aaaatggaag tttactaaaa gatgtaacta gatatggatg
    98761 cgagtatttc ttaggtattt taaaagatgg agatatcgtt tcaataacag gaggaagcac
    98821 tatgttggag ttttcaaacg ttattaaaac tgataagaga tacccaaact ctacaatagt
    98881 tccagctaga ggaagtatgg gaactgatgt agagatgcaa tctaataata tagttgcttc
    98941 gataagtaag aaattacatt ctaattgcaa acttttacat ataccagatg agttggatga
    99001 aagtgcaatg aaaactctta gccaagtgcc agagattaag acaactttgg agtatattaa
    99061 aagaacagac attttagttt ttagtatagg tagagcagat gtcatggcaa agagaagaaa
    99121 acttccagaa gacaaggttg atgagatatt atctaaaaat gcagttggag aagcatttgg
    99181 atattatttc aacaaagatg gagaaatagt ttacaaactg aacactgttg gaattgattt
    99241 agaaacagtt aaacaagtaa aagaaactat tgcaatcttt gcaggtactg aaaaagtaga
    99301 agccttatta gctatatcaa atatgcataa aaacatggtt cttgtaacag atgaagaaag
    99361 tgcttataag atattgtcat tattataaaa gtcaagacct agcacagagc tagaaaacat
    99421 acatttactt tataggaggt agcgaaaatg gttaaagtag ctataaatgg atttggaaga
    99481 ataggaagat tagcattaag aaaaatgatg gagcaacaag ataagttcga agttgttgca
    99541 ataaacgact taacagacgc taaaatgtta gctcatttat ttaaatatga tacagctcaa
    99601 ggaagattta acggagaaat agaagttaaa gaaggtgctt ttgtagtaaa cggaaaagaa
    99661 ataaaagtaa cagcagaaag aaatcctgct gacctaccat gggctgaatt aggagtagat
    99721 atagtattag aatgtacagg attctttaca tctaaagata aagctgaagc tcatatacaa
    99781 gcaggggcta agaaagttgt tatatctgca ccagctactg gagatttaaa aacaatagta
    99841 ttcaacacaa acagtgatat attagatggt tctgaaacag ttatatcagg agcttcttgt
    99901 actactaact gtttagctcc aatggctaaa gtattaaatg ataaatacgg tatagaaaaa
    99961 ggattaatga ctactataca tgcatacact aatgaccaaa atactttaga tggtcctcat
   100021 ccaaaaggtg atttaagaag agctagagct gctgctggaa acatagttcc aaacactaca
   100081 ggagctgcaa aagcaatagg cttagtaata ccttcattaa aaggaaaatt agatggggca
   100141 gcacaaagag taccagttgt aactggttca ataactgaat tagtttgtac attaggaaaa
   100201 aatgttacag ttgaagaaat aaatgcagct atgaaagaag cttcaaacga atctttcgga
   100261 tatactgaag aaatgttagt atcttctgac ataatcggaa taagctatgg ttcattattt
   100321 gacgctactc aaacaaaagt tatggaagta gatggaaaac aattagttaa agttgtttct
   100381 tggtatgata atgaaatgtc ttatacttct caattaataa gaactttagg atactttgca
   100441 caattagcaa aatagtcagt tttgagaaaa tagaaaagta gattgacaaa gtccgagttt
   100501 gtagtttaat actacggact caggactttt tttaagaaaa aatatataaa atacacctaa
   100561 ataatgttac atactatata taaaaatatg ataatatgat atatttaata gggtaaaagt
   100621 atgtactata ttatacttgg caataaaaaa ttactttatt aaaaggagag atatttatgt
   100681 caatgcttaa caaaaaaact atagaagata ttgatgtgtg cggaaaaaaa gtattagtaa
   100741 gatgtgactt taatgtgcca ttacaagatg gtgtaattac tgatgaaaat agattgaacg
   100801 gagctttacc aactattcaa tatttaattt ctaaaggtgc gaaagttata ttatgctctc
   100861 acttaggaaa gccaaaagga gaagctaagc cagaattatc tttagcacca gtagcaaaga
   100921 gattatctga gatgttagga aaagaagttg tatttgcagc agacgataat gttgtaggag
   100981 aaaatgcaaa gaaagcaact gaaaaaatgg aaaacggaga tgtagtatta ttagaaaata
   101041 ctagatatag aaaagaagaa actaaaaacg aagagaattt ctctaaagaa ttagcatcac
   101101 ttgctgaaat atttgtaaat gatgcgtttg gaactgctca tagagcacat tgctcaacag
   101161 ttggtgcagg tgagttctta caagagagag tttgtggata cttaattcaa aaagaattaa
   101221 aattcttagg agaagctgtt gcaaaccctg taagaccatt tacagctata cttggaggag
   101281 ctaaagtttc tgataaatta gctgttataa acgaattatt agaaaaagtt gataatctaa
   101341 taataggtgg aggaatggct tatacattct taaaagctca gggttatgaa gtaggaactt
   101401 ctttacttga aatagataaa gttgaatatg ctaaagaaat gatggaaaaa gctaagaata
   101461 agggtgttaa cttattatta ccagtagacg ttgttatggc agaccacttt gcaccagatg
   101521 ctactccaat agtaacagaa gatgctaatg taaaagaaga ttacatggga ctagatatgg
   101581 gacctaagac tatagcaaac tttgtaaaaa ctataaaaga atcaaaaact gtagtatgga
   101641 atgggccaat gggagtattt gaatttgaaa actttgcaaa tggtacatta tcagtagcta
   101701 gagctatggc agagttgact gatgcaacta ctgtaattgg cggtggagat agtgcagcag
   101761 cagttaatca attaggattt ggtgacaaga tgactcacgt atcaacaggt ggaggagcat
   101821 cactagaatt tttagaaggt aaagaattac caggtattgc agcattagat aataaataaa
   101881 ctaatatata gaggagattg taaaatgaga aaacctataa ttgcaggaaa ctggaaaatg
   101941 cataaaacaa taaaagaagc tttagaattt gtaaatgaag ttaaagataa agtaaattct
   102001 gataaagtag aagctgttat atgtgcacca tttactttat taaaggattt aaaagaagct
   102061 actaagggta caaatataaa gataggtgct caaaatatgc actttgaaga aaaaggagca
   102121 tttacaggag aagtttcacc tcttatgtta aaagaaatag atatggacta tgttgtaata
   102181 ggacattcag aaagaagaca atacttcaat gaaactgatg aaactgtaaa caagaaagtt
   102241 ttaaaagcat tagaagtagg aatagaccca atattatgtg ttggagaaac tttagaacaa
   102301 agagaagctg gaaaaactaa agatgtttgt aaagttcaag ttgaaaaagc tttagaaaat
   102361 gttcttaaag atgatttagc aaaagtagtt gttgcatatg agccaatctg ggctattgga
   102421 actggtaaaa ctgcaactgc tgaagatgct aatgacgtta tatcttatat aagagaagtt
   102481 ataaaaggat tatatggaga attagctaat gaagttagaa tacaatacgg tggaagtgtt
   102541 aaaccttcaa atgtagccga aataatgggt caaagtgata tagatggagc tttagtaggt
   102601 ggagctagtt tagctagtaa tgattaccta gaccttgtta atttctaagg agtgaaatga
   102661 tgaaaaaacc agttgcttta attattatgg acgggtttgg atacaataag gatgttaagg
   102721 gaaatgcaat tgctgagtcc aaaactccta atttagatag aataaaaaaa gagtatccaa
   102781 acacattgat aaatgctagt ggtcttgatg ttggtctacc tgatggtcaa atgggaaatt
   102841 cagaagttgg acacactaac ataggtgcag gtagaatagt atatcaagat ttaactagaa
   102901 taacaaaatc tataaaagat ggtgactttt ttactaataa agttttatgt gaagcaatgg
   102961 ataatgcaaa agaaaattct cttcatgtaa tgggactttt gtctgatgga ggagtacatt
   103021 cacatataga ccatcttaag gctataatca aaatggcaaa agataaaggc gtacaaaagg
   103081 tgtatgttca cgcatttact gatggtagag atactgaccc tcaaagtgca ttagaatatg
   103141 ccaaagaagt acaagctagt atggatgaaa ttggagtagg tgaatttgct acagtttctg
   103201 gtagatacta cgctatggat agagataaga gatgggaaag agtagaactt gcatacaatg
   103261 ccatggttag aggtattggt gaaaaagcaa attctattga ggaagctatt caaaactctt
   103321 atgatgatgg aaaaaatgat gagtttataa tgccaacagt tattatgaaa gatgataaac
   103381 cagttggaag tataaaggaa aatgattcaa ttatattctt caactttaga ccagatagag
   103441 ctagacaaat aactagagct ttagtttgtg aagagtttga tggatttaaa agagaagata
   103501 ttaaaaactt ctttgtatgc ttaacagagt acgatataac tatagagaat gtgcatatag
   103561 catttggacc tcaatcttta gccaacactc taggtgagta tctagctaaa aatggaaaaa
   103621 ctcaacttag agctgctgaa actgaaaagt atgcacatgt aactttcttc ttcaatggtg
   103681 gagtagaaga gccaaataaa ggtgaagaaa gactattgat accttctcca aaggttgcaa
   103741 cttatgattt aaaaccagaa atgtcagcat atgaattgac agataaagct ttagataaat
   103801 taggagaaga taagtttgac tttatagtat taaactttgc aaatcctgat atggtaggtc
   103861 atacaggaag tatagaagcg gctataaagg ctgttgaaac tgtagatact tgtgtaggaa
   103921 gattgataga taaaatagta gagttaggtg gaagtgcaat aataactgct gaccacggaa
   103981 atgctgaata tatgttagac ccagaaacag gaaaaactgt aacggctcac tctattaatc
   104041 cagtaccatt tattgtagtt ggtcaagaat atgaaagtgc aaaattattg gatggaggaa
   104101 gattatctga tatagcaccg actgttttag atatgatgaa gttagaaaag ccagaagaaa
   104161 tgactggtca ttcacttatt tcaaaatagt cgattctgat aagtagagaa taatatataa
   104221 actacaatat aaatttaaaa ataatgcagt ttaggtttat tttgtaaagt ttagacttta
   104281 tgacagaaga tttagatttt gtaagttttg gatattgtaa ggtgtagatg aagatatatt
   104341 ttataaaatt tacaagatag acaatttata aaataaaaaa ttataatata gataataatg
   104401 taaataatga tataaataat aacaagatag acaataacaa tataaataat aataattaat
   104461 atatattata taagcaaaaa ttttagaatc attagttgta cattttatgt ttaatgtttt
   104521 aaatctagtt ataatatttg tgtatttaag attggttttg gattttagac aagttttttt
   104581 atattatata aattaaaata ttataaaaaa taaaataaaa ataggaactt ttacaaattt
   104641 acccatcaaa taaatttgta cctaattttc aaaagcaact ttatgtacta taatatagaa
   104701 agtagattaa aatagtagac ggatgtttac agtaaatgaa aatttaacta atagaaaatc
   104761 gaagggagat atacaaatgt cagttattga attagtatat gctagagaag tattagactc
   104821 aagaggaaat ccaactgtag aagttgaagt agtattagaa gatggagcaa tgggaagagc
   104881 aatagttcca tcaggagcat ctacaggagc ttttgaagct gttgaattaa gagatggaga
   104941 taagggaaga tacttaggaa aaggtgtaga aacagctgtt gctaacgtaa atgaaataat
   105001 agcacctgaa atagaaggaa tggatgcatt tgaccaacca gctatagatg caatcatgat
   105061 agaattagat ggaactccaa acaaaggtaa attaggagct aatgctatat taggtgtttc
   105121 aatggcagta gctagagcag ctgctgatga aataggttta ccattattcc aatacctagg
   105181 tggagtaaat gctaaacaat tacctgttcc aatgatgaac atattaaacg gtggagagca
   105241 tgctgataac aacgttgatg ttcaagaatt tatgatatta ccagtaggag cttgttgttt
   105301 caaagaaggt ttaagaatgg gagcagaagt attccattcg ttaaagaaag ttttaggaga
   105361 aaaaggatta gcttgtggtg taggtgacga aggtggattt gctccaaact taggttcaaa
   105421 cagagaagct ttagaattaa tagttgaagc tataacaaaa gctggatata agccaggtga
   105481 agacgttatg ttaggtcttg acgttgctgc tacagaaatg tacaacaaag aaactaaaaa
   105541 atacgtttta gcaggagaag gaaaagagtt aactgctgct gaaatggttg ctttatatga
   105601 agattggtca aacaacttcc caataataac tatagaagat ggtttagacg aagaagattg
   105661 ggatggatgg aaattattaa ctgaaaaatt aggaaataaa ttacaattag ttggtgacga
   105721 cttatttgtt actaacacag agagattaga aaaaggaatt gaaaatggag tagctaactc
   105781 tatattagtt aaagttaatc aaataggtac aataactgaa actttagatg ctatagaaat
   105841 ggctaagaga gcaggatata ctgctgttat atctcacaga tcaggagaaa ctgaagattc
   105901 tactatagca gatttagctg ttgcagtaaa tgctggtcaa ataaaaactg gtgctccatc
   105961 aagaactgat agagttgcta agtacaacca attattaaga atagaagaaa tggttggaga
   106021 gcaagctaga tattgtggat taaaatcttt ctataactta aaaaaatagt tattaaaatg
   106081 gttactaaaa aagattaatg aagagagaat ataagatatt taagctcatc ttataagata
   106141 taaaagagat aagtgttaat tgatttttca attcacttat ccttttttat attttattac
   106201 ttaattttat tcatagattc agaaaaaata tgaataaatg tttctatctc atttttatct
   106261 aattgcatag gaggttttta ttaaaaaata aaagtgaaga catattttga tttaatcata
   106321 attgtcatta aagtattatt gctaaatgtg gattatttat tttaaattat gttgaaatta
   106381 aaagttatat caatatttta aaatgattgt tttattttgg tggctattta taatatatac
   106441 aatgagagta gattatttta agtgttaatt tatgcattaa tttaaatagt tttctcttct
   106501 aagttttatt catttttata ttgagaatat agtaatatat tatagtaaca atatattttt
   106561 ttgtatgtta tttatagcat aatagtcttg aaatggctat attattgtga tacactaaag
   106621 agtaatgaaa tttaagtaat aaacagatag gaggggcaag aatgagcaac attttaatgg
   106681 gtgtgcaagt agtactaggg attttactag taattagcat aatgcctcaa gattctaaga
   106741 atgctttacc ttcagaattt ggtggagaag gaagccaagc ttactttaaa ccaaaaggaa
   106801 aacaagcatt tcttgctaga gttacaaaga taacagcagt attatttttt ataaatgcat
   106861 tggcattact tatagttaag aagtagtgat attgttgaat aactggccat tatttaatta
   106921 gtgcatttgc tagacattta aaaattaaat ttagttttta ataaaagtac atcaacataa
   106981 aaagtacgta aaataaaagc tggactaatg attttatatg attagtccag tttttatttt
   107041 atagattgac tatttgttgg cgtttattat ttgtatttat atattgtttg tatttatata
   107101 tttttttcaa actaaaaaat atatagcaac actactttca ccatgtttaa tgagataata
   107161 tacacaaagt tatattatat agtaaaaata gtataataat atgtttttac gcaattaaat
   107221 cgattttata tatgtttctc ataataccaa tttgtgttat caaatatcat taaataagat
   107281 tattctgctt taaaataaat attattaaaa atactgtaat tatagaaaat ccagaagtaa
   107341 atactacaat ttgtccagct aattcactgt cagaatccat ctgttgtgcc attgtaaatg
   107401 aagaaactgc agttggagca gataacatta tcataagtga agctaattct acatttctaa
   107461 atccaagaaa aatgctaatt ggtaaaaata tagcaggtac aagtataagt tttccacata
   107521 cgcccaatat aatggggttt atatgcccct ttatagcaga aaaactaaaa gagccaccta
   107581 gaattataag tgataaagga gtagctattt tagaaatatc accaatagtt ttgtgtaaaa
   107641 atgtaggtat attaatattt aaaattagca ttgtcaatcc tataaaagag gcgattatga
   107701 gtggatttgt taagacgctt ttaatcatat gttttatatt tactttgcca cctctaaaaa
   107761 tttcaagaat tacaacagat aatacattaa acataggtac aataactgca attaataaag
   107821 atgtgactcc aatactctta tcgccaaata gtgaaattgt cacaggaata ccaaatataa
   107881 cgaaattgct acgaaataca ccttgtatta atgcacccct tgtcttatta tctttttcaa
   107941 ttttaggaat aataaagact aagattaaga agattgaaaa aataattata acagctacag
   108001 ccataagcct aaaattaaga gaaccatcta aatctgtatt gtatatatta aaaaataata
   108061 ataatggcaa gaaagacttg aagcagacat tattcatttt atttaaagta tgagtatcaa
   108121 atagacctaa tttttttaga acatatccaa gtgacattgt gagaaacaaa ggaagaacaa
   108181 catttattga taggattaaa ttttccaagg tttactctcc gtgattctat gatttgataa
   108241 ttcaattaaa tttccattag tttttttcaa ataaatagat ttcatattac caaagacacc
   108301 tgtacattct acaattcctc gcttaatttg aatctcattt tgttctaagt atttttttgg
   108361 ctcatcaata tttgtgttta ttttaaaaca taagttatct ctacttgctt gtaaattttt
   108421 aacataaggt aaaaatttat aaccttttat gttaagattt atcttaatat ttctagcatt
   108481 aagtttataa tttttgctca tttctttaga tgaaaaacca aattttttat aaaaattgat
   108541 ataattttct atattattat tagtaatgac cacatgttct atttttttta tcatggcaac
   108601 tcctt
//
LOCUS       DAEPIN010000005        98576 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.005, whole genome shotgun sequence.
ACCESSION   DAEPIN010000005 DAEPIN010000000
VERSION     DAEPIN010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 98576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 98576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..98576
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.005"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            109..300
                     /locus_tag="KR250_000421"
     CDS             109..300
                     /locus_tag="KR250_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HBH0066390.1"
                     /translation="MKKVALILAIIGALNWGAIGILGTDLIGSIFGGTYEMVSRIIYF
                     IVGLAGLYLIPSLMSDDRE"
     gene            455..1864
                     /locus_tag="KR250_000422"
     CDS             455..1864
                     /locus_tag="KR250_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBH0066391.1"
                     /translation="MFIINELNKIVNEEVISFHMPGHKKGQVYKKLGYKDILKNLYKM
                     DTTEILGTDNLHFPEGIIKNSQKKASRVFKSDYTCYLVNGSSCGIQSAIMSVCSPKSK
                     IITNRDCHQSVINGCILGDIDIEYVPCEISEETNILKGISISNVIDIIDKNLDAKAVL
                     LTYPTYYGMTYDLEYICNYAHSKKMVVIVDEAHGAHLGLSERLPKTALEQGADIVVQS
                     THKTLPAFTQSSMIHIQGDRVDIDKILNMLRITESSSPSYLLLSSLELAVDIYENKGK
                     ELMEELLNNIITFKNKVDSNINIYDTNDKTKIFISSKNMGLTGYELENILRNKYNIQV
                     ELSNYYGVLLICTIGNTAEDFSSLETALNNVVKEDFKFEKLENKKYPVVIPKKILTPR
                     EAFYKTKKSVKIYDSIGKVCGECIVPYPPGISVISPGEVISKEIIDYLMFCHSKGMIV
                     SGLKDVDLNFIQVIDLEDI"
     gene            1995..2684
                     /locus_tag="KR250_000423"
     CDS             1995..2684
                     /locus_tag="KR250_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HBH0066392.1"
                     /translation="MKGKLIIIESGSDASGKATQTEILYERLKKEGYMIKKVEYPNYE
                     SPSSALVKMYLKGDFGKNASDVDPYIASTFFTADRYASFKTDWEEFYNEGGIIIADRY
                     TTSNMVHQASKMDICDREEYLNWLADYEFNLFKIPQPDCVIFLDVPIEFSKKLMENRK
                     NKITGEEKKDIHESDIEYLEKSYNNALYIADKYDWKKINCVLDNKLRSIESIHNEIYK
                     IVLDSIKPMER"
     gene            2687..3622
                     /locus_tag="KR250_000424"
     CDS             2687..3622
                     /locus_tag="KR250_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HBH0066393.1"
                     /translation="MYFENIIGQDFAKKYLTNSIKKNKLNNAYMFEGMDGIGKKKFAD
                     ELSKLLLDYENLENSPDYVLIKPDGNSIKIAQIRNLQSDIVIRPHKDYKIYIINNAEK
                     MTVEAQNALLKTLEEPPNYAIIILVTNNKESLLETIKSRCDIIKFSPIPIEDLKRYLI
                     NTGIEEERAQLLAIFSRGSIENALNLSQSSEFSMMREDIQQYIQIMLDKNIVEILNIP
                     NNMEKYRGKIIALLDMMINYFRDIILLKENVNKNMLINVDKLVFIQNMNGKISYSQLS
                     KIIDIIEDAKSKIKSNCNFNISIQVMSLNIYEVIK"
     gene            3619..4512
                     /locus_tag="KR250_000425"
     CDS             3619..4512
                     /locus_tag="KR250_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HBH0066394.1"
                     /translation="MIKIVGIRFKSAGKIYYFDPVDFNIEQDMDVVVETARGLEYGKV
                     VVGPKDMDESELVSPLKPIIRIATEEDKKVYLENKEKAKETFELCQQKIKEHELTMFL
                     IDCEYTFDRNKLIFYFTAEGRIDFRELVKDLAAIFKTRIELRQIGVRDEAKSIGGLGP
                     CGRKLCCSSWLGDFQPVSIKMAKDQSLSLNPTKISGICGRLFCCLKYEHDVYSEAIEK
                     MPVVGALVQSDDGKGKVVEVNPLLEQIKVEFQDKTVKIYTREDIKILREPKKCDGCGG
                     KCKDNDGLDEATLRELKKLED"
     gene            4590..5336
                     /locus_tag="KR250_000426"
     CDS             4590..5336
                     /locus_tag="KR250_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HBH0066395.1"
                     /translation="MQLKDSERIDDLQLKGLKIIQDTNGFCFGIDAVLLANFTKIKKD
                     AKVVDLGTGTGIIPILIAGKSEAKKIIGVEIQEDVYEMATRSIKLNGLEERVEIINED
                     IKYIDKVLDINGYHVVTSNPPYMHIDGIKNPNDKKAISRHEVRCNLEDVIRAASRLVM
                     PRGKFFMIHRPTRLVDIITLGRKYKLEPKVIQFVHPRPQKAPNLVLVQFVKDGRPELK
                     ILDPLYVYGEDGNYTKELKAIYNNKDIGEL"
     gene            5337..6170
                     /gene="rsmI"
                     /locus_tag="KR250_000427"
     CDS             5337..6170
                     /gene="rsmI"
                     /locus_tag="KR250_000427"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HBH0066396.1"
                     /translation="MSGKLYICPTPIGNLEDITYRTLRILKEVDLIAAEDTRHSIKLL
                     NHFEISKPLTSYHEHNKDSKGDYLINKLIDGENIALISDAGMPGISDPGEEIIKQAIQ
                     NNIEIEVLPGATAFVTALVGSGMDTHRFIFEGFLDRDKKVRKAQLEELKEESRTIIFY
                     ESPHRLKDTLKDMLKILGNRNISINRELTKKYQEIIRENIETTIKIFEEKEVRGEFVL
                     IVEGFHGEKSKKENYDGLTDREYVLKLIESGMNKKDAIKIVCKDRKLKKDVVYKQVLD
                     L"
     gene            6193..6897
                     /locus_tag="KR250_000428"
     CDS             6193..6897
                     /locus_tag="KR250_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HBH0066397.1"
                     /translation="MEIGKIIIESLKREDTHKIFIELDNKHELEKIIPKISCMKSVGE
                     CKYHVVNCFEHSINALKELEIVLNDKDFFPIHLRKHVSNYLKTYIEEGINKLHVLKLG
                     TFLHDIGKPDSMTLDETGRVHFTNHEKIGAQIIDNMGIKLELSTETYRLISKYVRYHM
                     ILLSLYKKNDLSRKELINVFNLVDEDTIGVIMLGYADIVSTVKLLGKSGEVSVLKTYM
                     EYILTNYLYKSNYTHV"
     gene            complement(7085..7513)
                     /locus_tag="KR250_000429"
     CDS             complement(7085..7513)
                     /locus_tag="KR250_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007287587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HBH0066398.1"
                     /translation="MDVTDYRIIEILQDDGRISMKDLGKIVGLTSPAVSERVKRLEES
                     GVIEGYKAIVNPDSLGRVIKAFIHISLPSNGYTEFIESAAKDPRIVECHHITGDDCLL
                     LKVIVKDMYELENVIDTIKKIGSTKTSVILSTPIQAKSIL"
     gene            complement(7550..8980)
                     /locus_tag="KR250_000430"
     CDS             complement(7550..8980)
                     /locus_tag="KR250_000430"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HBH0066399.1"
                     /translation="MRNLTLLTDLYQLTMLNGYFEKNIHEDIVVFDMFFRKNACDGGY
                     TIVCGIDQVVEYIDNLHFSDEDLEYLKNLNLFSDKFLKFLKEFKFTGDIYAVEEGTIM
                     FPNEPLITVKAPLYQAQLIETALLTIVNFQSLIATKASRVCFAAQGDPVFEFGLRRAQ
                     GPDAGIYGARAAVVGGCAGTANVLAGKMFDIPIIGTQAHSWVQKFDNELEAFQAYADV
                     YPDKCLLLVDTYDVLNSGVPNAIKVFKNISEKGYKPMGIRLDSGDLAYLSKEAKKQLD
                     NAGFSDISITASNDLDEYTITSLKAEGATINSWGVGTKLITSFDSPSLGGVYKLAASC
                     EKGVLEPKIKISENPEKINNPGYKKVIRIYNEDNKAEADLIMLHDEVIDESKPLEIFH
                     PTYTWKTKVFTNYKVKELLKPLYIKGRCKYNKKAVLEIKNHVQYELSTIWEQYKRLSK
                     PHIYKVDLSRNLWYLKTQMIDSKKVL"
     gene            9112..9696
                     /locus_tag="KR250_000431"
     CDS             9112..9696
                     /locus_tag="KR250_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein A"
                     /protein_id="HBH0066400.1"
                     /translation="MIIIGKIWFYMVSIGIIGSLISKNMGELNKVILSETSKGIEFAI
                     SLAGIMALWMGIMNIAKESGLIEKIGQKLNPIMRRLFPSIPKGHKAMSYIVMNIALNM
                     LGAGNGATAFGLKAMSELQTLNNKKDTATNDMIMFMVINISSIQIIPFTIIKLRMDMG
                     AQNPSEIIFTTLFATIISTAVAIITCKFFQRRYR"
     gene            9712..10233
                     /locus_tag="KR250_000432"
     CDS             9712..10233
                     /locus_tag="KR250_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein"
                     /protein_id="HBH0066401.1"
                     /translation="MEIFSNLLIPIIILYIVVYGKYKKIDVYDSFVKGAIDGLKAAWD
                     ILPYIIGIFLAIGIFKTGKGLDMLEWLFTPIANMMSIPKELIGLISVKPLSGSGALGM
                     YSELANRVGIGSLVEKMGATIVGSSETIFYTMAIYYGSLKIKNTRHTLSCAMISHVAG
                     VIAAVFICYVIFV"
     misc_binding    10299..10545
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            10629..12566
                     /gene="metG"
                     /locus_tag="KR250_000433"
     CDS             10629..12566
                     /gene="metG"
                     /locus_tag="KR250_000433"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HBH0066402.1"
                     /translation="MSKPSFYVTTPIYYPSGGLHIGHTYSTVAADTIARFKRFCGYDV
                     KFLTGTDEHGEKIQKKAIEQGMSEIEYLDGMIKDIKALWNTMDISYDDFIRTTEKRHT
                     DIIQKIFTKLYEQGDIYKGEYEGRYCTPCESFWTESQLLEGNKCPDCGRETYLVKEES
                     YFFRLSKYEDRLKELFKDDSFCFPAARKNEMVANFLDKGLEDLSVTRTTFDWGIKVPF
                     DEKHVIYVWVDALCNYITALGYMTDNDEEFKKYWPANVQIVGKEIVRFHTIIWPALLM
                     ALGLEVPKQVFGHGWILFADDKMSKSKGNVVYPEPIIERYGIDTLKYFLLREFAFGQD
                     GSYTHRNFVTRINYDLANDLGNLISRTVAMVEKYNNGIIPTAKVSTNFDADLKEQAVS
                     TRENFEAEMDKMQFHEALESVWKLVRRTNKYIDETMPWALAKDETKKDELDTVLYNLC
                     ESIRIIATLINPIMNETANKIYEHIGIKGQDDITTWESTKTFGLIGENVKVFKGEPLF
                     PRLDVEKEIEELTKMFSGKPPVEEKPLEHKEEITIDDLDKIELRVGKIISCEKHPKAN
                     KLLVSQVKIGPETRQIVSGIAEYYKPEDLVGKEVTVVCNLKPVKLRGVESQGMILAAG
                     DDGDPYVLPFTQGAKDGCEVR"
     gene            12596..13366
                     /locus_tag="KR250_000434"
     CDS             12596..13366
                     /locus_tag="KR250_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HBH0066403.1"
                     /translation="MLFDSHAHLNDESFDEDRDELIGSLKDKGVDLVVNPGADIETSI
                     TAIELAKKYDFIYSAVGVHPHDVSKLDDTAIETLRKLATENEKVVAIGEIGLDYYYDY
                     SPREEQKEWFKKQIELANELKLPIIIHDRDAHGDTFEIIKNTKNPEIGCVLHCYSGNV
                     ELAREYVKMGCYISIPGTVTFKNNKKTREVVREIPLERLFIETDSPYMSPEPHRGKRN
                     NPSQVSFVADKIAQEKGISYEEVCRVTKENAKKFFNIK"
     gene            13703..14134
                     /gene="phnG"
                     /locus_tag="KR250_000435"
     CDS             13703..14134
                     /gene="phnG"
                     /locus_tag="KR250_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HBH0066404.1"
                     /translation="MYTVDSTKILINMNLEKLEKIYSMIKDTSSISIIKEPNLATVMV
                     RANESVKNTTFNLGEILVTECSVKVDESLGYGIVSENNNKKAIYLAVIDAVLHSNNSK
                     FDELKNYINKSVYEENLRYEQEIIDEFSLINKTKVQFNAMD"
     gene            14149..14724
                     /gene="phnH"
                     /locus_tag="KR250_000436"
     CDS             14149..14724
                     /gene="phnH"
                     /locus_tag="KR250_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HBH0066405.1"
                     /translation="MVNYTQEIYRKMLDSASFPGKLNNIDNINIENNTKLLNGAIGIA
                     ITLLDQEVTFYIENYTQKDIKNIRALTMSNQIDYRKSDYIFLDINSEVDILQIKIGSF
                     EYPDESATIVHQVNDLSTYSQTKYIKLDLSGPGIKSKNSIYINGIREEFIERLSSINK
                     DYPIGIDLILVDKKGEIAFIPRSSKLSWEVL"
     gene            14727..15812
                     /locus_tag="KR250_000437"
     CDS             14727..15812
                     /locus_tag="KR250_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-phosphorus lyase complex subunit PhnI"
                     /protein_id="HBH0066406.1"
                     /translation="MAYVAVKGGEEAILQAKNILEFYRVKGDSLPIDVKQIEEQMSYA
                     VDKVMSEGSLYNKELSALAIKQAQGDILEASFYVRALRSTMPRITYSEPINTEEMRIK
                     RRISATFKDIPGGQYLGATRDYENRILNVELLEEDEKKNRHFRDSFINELETVDEIPE
                     FTKISNIFRKENLLQEEVCENKLEEVIDDITRNSLRFPVSRSAKLQALTRGESGVVSG
                     FAYAIVRGFGDEHPYISELRTGNVFIKVKHPIYEDEEIIIGDMLVTECEIVSKASQNK
                     TENSKTNSEDKNKMSLGYGLCMGNNENKAISMAILDKSLEPNSEKLAQDEEFVLLHID
                     GIDSLGFISHFKLPHYVDFKADVERIK"
     gene            15832..16701
                     /locus_tag="KR250_000438"
     CDS             15832..16701
                     /locus_tag="KR250_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HBH0066407.1"
                     /translation="MIYNYGFLDENTKKEIRRKILKAISIPGYQVPFGSRELPIAKGW
                     GTGGLQLTLSLIGKNDVVKVIDQGSDDSTNACNIRSFIASVSNVETTKDTLEATLIQT
                     RHRIPEEKLKSNQILIFQVPIPETLRIVEPSEVETRRMHSEEDYSRMWVYLYEDIVRF
                     NDISIAVEYPCKVNDRYLMNPSPIPRFDIKKLNMSDNLFLFGAGREKRIYAIPPYTKV
                     EPLEFEDYKFEEEKFEGKYCSLCNSTNTFLDEVYDSDTNEKYYSCSDTSYCEKVRLKN
                     NSIDVTIGGTWNE"
     gene            16694..17560
                     /locus_tag="KR250_000439"
     CDS             16694..17560
                     /locus_tag="KR250_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBH0066408.1"
                     /translation="MSKINDSKRLENIDKQVLIGRNINLIYGDGCEKCFESTGYESNN
                     ICKYCGSIVACNNVNLELYEGEVLGIVGESGSGKSTLLKILFFQEHANSGEVYISHYN
                     KDTNILELSDQKKRYIKNHFMGIVYQNPHLGLNLDFSSGGNIAEKLLMANLYNVEKIR
                     DRGKELLIKTNIPVERIDHKPKYFSGGMQQRVQIAKALSNNPPILLLDEVTTGLDVSV
                     QAEVLDLIREIQRELKISMIVVSHDFDVIKMLADRTIVMKNGSVIESGLTDQIMEDPQ
                     HPYTQQLINSLL"
     gene            17601..18314
                     /locus_tag="KR250_000440"
     CDS             17601..18314
                     /locus_tag="KR250_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBH0066409.1"
                     /translation="MKETILEVNNFSKDFELHALNKTITACSNISFEVSKGEFLGIIG
                     KSGAGKSTILKSIYKTYIPTTGEIIFNSEIYGNVDLAKIGDREMINLRKKEIGYVSQF
                     LKTLPRITAIELVVHSLIESGFEKDCSYDMAKDILNQFEIKENLWDAYPNNFSGGEKL
                     RLNLAQAMVKKPRLLLLDEPTASLDNQSKIYVKEKMLELKSQGTTMIGIFHDIEFMET
                     VIDKTFTMTKGTISESGVA"
     gene            18315..19484
                     /locus_tag="KR250_000441"
     CDS             18315..19484
                     /locus_tag="KR250_000441"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HBH0066410.1"
                     /translation="MIKLIHNANIVLEDMILENGHLIIEGDTIKKVSNDKIDYAFDFN
                     EIDEIIDAKNLYVIPGIIDIHSDAIEKEIEPRPSTLLPFNMAFYELDKKLPANGITTV
                     YHSISLGDGVGVRSIDNSLKMIKNIDSYKNIDSKSINHKVHLRYEVLYYEGLEKVLEL
                     LDENKIDYLSIMDHSPGQGQYTNPTFYKEYATKVWGVTENYVDTWLDDLVNLHDNLDW
                     NKIANIIGIAKTKNINVASHDDDTLEKMDFIKNIGINVSEFPITLEVAKHSKKLGVLT
                     CLGAPNIVRGKSHNNNLKAMDAILEDCCDIVCSDYLPSAMIKSMCIVAERINNLNKAV
                     SLFTSNPAKAVGIYDERGSIKENKKADLVLVDIDSEYPKVVNTIVNGKTVYKREV"
     gene            19488..20324
                     /locus_tag="KR250_000442"
     CDS             19488..20324
                     /locus_tag="KR250_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HBH0066411.1"
                     /translation="MLTELHCHTSISDNNFTTEEIIKKAAEKNINILAITNHDTLSGL
                     DEALVLGEHYGITIIPGIEISAYDYKNRKRVHILGYNIDLNSQEIKNLCNPMVCDRHK
                     ASVKMVNKIIDLGYKISLEDVKKYSSKTGIFKQHIMKALIDKGYTDNIYSNLYKELFH
                     RGNGKVYVSLKYVDYRDAIKAIKNSGGICVLAHPGQMDNFSAIEEMVKVGLDGIEVYH
                     PSHNKELEKESLSYAKKYNLVVTGGSDYHGFYSQHNYELGSKSLNKEDLFKFRLALGG
                     VL"
     gene            20325..21320
                     /locus_tag="KR250_000443"
     CDS             20325..21320
                     /locus_tag="KR250_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBH0066412.1"
                     /translation="MSFLQVKNVGKSYGQVKVLKDISIDIEKGEFICLLGPSGCGKST
                     LLRIIAGLEDKHGGKIIINDKDMTNSPPESRNFGIVFQSYALFPNMNVYKNIAFGLEN
                     KNISKSNIDKKVKEVLEVVELSGYEKKYPSQLSGGQQQRVALARAIALEPDFLLLDEP
                     LSALDAKVRLKLREQIRSLHRKLGITTIMVTHDQEEALCLADKMVVMNRGEIIQVGTP
                     KEVYKNPETPFVADFIGTINFIDDGINKIAIRPEDIKVESNRDSKDKDIKVGEILDIE
                     FRGFNYRITVEYRSKQMKLDVVSKVAEQMKLCIGSKINFKIPKEGIVQYKSEGCA"
     gene            21338..23026
                     /locus_tag="KR250_000444"
     CDS             21338..23026
                     /locus_tag="KR250_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     permease subunit"
                     /protein_id="HBH0066413.1"
                     /translation="MITSKNKRRISDSTIQKYFLIFSVIVLIVFILFPLVSLLKNAFM
                     DTSGNFIGISNFSKYVENPSLIASFKNSIFVSTVSSLISLCLAFIYSYAITRTNIKFK
                     DFFKTMGMFPLFAPTMLYGISLIYLFGNKGIFTTMGLEVPLYGPLGIIISQILFTFPQ
                     AFLILSVALSMADYRLYEAADSLGASEFKKFCKITIPGIKYSLLSSFFVSFILAFTDF
                     GAAKVVGGNYNVLATDIYKQVVGQFNIPMGATVSMVMLIPVLIAFTLDKMASKKQGMT
                     ITAKSMPYKIKENKFRDRVFLIVCSFITLLIFILIFVSVIGSVIKLWPYNLSFTMEHY
                     TFKGILGSGIKTYLSSLKISTLTAIFGTIFVFFSAYMIEKVDKFPKLRQTAYLLSMIP
                     MALPGLVLGISYITFFNSASNPLNFLYGSTAILVIVNVVHFYSVCFITSNSSLKMLDK
                     EYELVAKSINIPFYKVFFNITVPMSITSILEIVVYYFVNSMVTVSALIFLYTPQTQTA
                     SISILKLDEIGYIGTSAAMAVLVLLTNIIVRLLYEFVTKKLKNNTQKWQQKDVA"
     gene            23038..24105
                     /locus_tag="KR250_000445"
     CDS             23038..24105
                     /locus_tag="KR250_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HBH0066414.1"
                     /translation="MKFKKLVSLGVLSTLLLSVITGCSTDSSGSDSKSDSKTKGDLTV
                     YTAIEEDSIEPYLATFKEKYPDIKLNIVRASTGDITARLLAEKDNPQADVVWGVAATS
                     LLVADDQGMLEPYAPKGSEEIESAFKDDKEVPSWVGIDAWMTGITVNTKELSDKNIPV
                     PSSYEDLIKPEYKGLISMPNPSSSGTGYLTVSALIQIMGEEKAWQYMDKLHENIGVYT
                     QSGSAPATSAASGEYPIGISFGYRGIKLKEEGYPVEVVFPKEGSGWDIEANALVKKDN
                     IKEASKVFLDWAISKDVMNEYSKNYAVTTISTGNPIPEGFPKKPLEQMIDNDLKSAAK
                     NREDILNKWISKYDGKTEKES"
     gene            24542..25537
                     /locus_tag="KR250_000446"
     CDS             24542..25537
                     /locus_tag="KR250_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBH0066415.1"
                     /translation="MSFLQVKNVGKSYGQVKVLKDISIDIEKGEFICLLGPSGCGKST
                     LLRIIAGLEDKHDGKIIINDKDMTNSPPESRNFGIVFQSYALFPNMNVYKNIAFGLEN
                     KNISKSDIDKKVRDILEVVELSGYDKKYPSQLSGGQQQRVALARAIALEPDFLLLDEP
                     LSALDAKVRLKLREQIKSLHRKLGITTIMVTHDQEEALCLADKMVVMNNGEVIQIGTP
                     KEVYKSPKTPFVADFIGTINFIDDDVNMIAIRPEDIKVEFNRDFKEIDVKVGEILDIE
                     FRGFNYRITVEYCSKQIKLDVVSKVVEQMKLCVGSKINFKIPKERTIQYKSEGCA"
     gene            25555..27243
                     /locus_tag="KR250_000447"
     CDS             25555..27243
                     /locus_tag="KR250_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     permease subunit"
                     /protein_id="HBH0066416.1"
                     /translation="MITIKNKRRISDSKIQKCFLVFLVIALVVFILFPLISLLKNAFM
                     DTSGSFVGISNFSKYIENPTLIASFKNSIFVSTISSLISLCLAFIYSYAITRTNIRFK
                     NFFKTMGIFPLFAPAMLYGISLIYLFGNKGIFTIIGIEVPLYGPLGIIICQILFTFPQ
                     AFLILSVALSMADYRLYEAADSLGASEFKKFFKITIPGIKYSLVSSFFVSFILAFTDF
                     GAAKIVGGNYNVLATDIYKQVVGQFNIPMGATVSMVMLIPVLIAFTVDKIASKKQGMT
                     ITAKSMPYKIKENKFRDRVFLIACSFITLLIFILIFVSVIGSVIKLWPYDLSFTMEHY
                     TFKGISGNGIKTYLSSLKISILTAIFGTIFVFFSAYMIEKVDKFPRLRQISYFLSMIP
                     MALPGLALGISYITFFNSASNPLNFLYGSTTIMVIANIVHFYSVCFITSNSSLKMLDR
                     EYELVAKSINIPFYKVFFYVTLPMSITSILEVAVYYFVNSMVTVSALIFLYIPQTQTA
                     SISILKLEEIGYIGPSAAMAVLVFLTNIIVKLLYELTTKKLKTKTQKWQQKEVA"
     gene            27255..28322
                     /locus_tag="KR250_000448"
     CDS             27255..28322
                     /locus_tag="KR250_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HBH0066417.1"
                     /translation="MKFRKLVSLGILSTLLLSVITGCNNASSGSNLKSNNKIKGELTV
                     YTAIEEDSIDTYLTTFKEKYPDIKLNVLRLSVGDITARLLAEKDNPKADVLWGVAATS
                     LIILDDKEMLEPYAPKGFDKVNPVFKDDKKVPSWVGIDAWMTGITVNTKELSDKNIPV
                     PSSYEDLIKPEYKGLISMPNPASSGTGYLTVSELIQIMGEEKAWQYMDKLHKNIGVYT
                     QSGSAPAILAASGEYPIGISFGNRGIKLKGEGYPVEVVFPKEGSGWEIESNALVKKDN
                     IKEASKVFLDWAISKDAMNEYSKNFAVTTIDTGNPVPEGFPKNPLEQMIDNDLKSAAR
                     NREAILNKWIEKYDGKTEKES"
     gene            28514..29044
                     /gene="rnmV"
                     /locus_tag="KR250_000449"
     CDS             28514..29044
                     /gene="rnmV"
                     /locus_tag="KR250_000449"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HBH0066418.1"
                     /translation="MIKEIIVVEGRDDVTAVKRAIDAELITTGGFGFPKGVMERIKAA
                     NERRGVIIFTDPDFAGEKIRKKIASEVPGCKHAFLPREEAKKDGDIGIENATPQSIIA
                     ALNKVRTENTEKRDEFKQVDLIRNGLIGNEEASLRRDALGKILGIGYGNAKQFLNRLN
                     NYGVEREEFIDALKQI"
     gene            29215..30084
                     /gene="rsmA"
                     /locus_tag="KR250_000450"
     CDS             29215..30084
                     /gene="rsmA"
                     /locus_tag="KR250_000450"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HBH0066419.1"
                     /translation="MDRLSSHNATKEVVQKYNFKFSKSLGQNFLIDSNIIDKILSGAR
                     ITRGDNIIEVGPGIGTLTREMGKIAEKVVAIEIDRNLIPILKDTLSDLDNTEVVNQDI
                     LKVDIQELVKDKLNGGPVKLVANLPYYITTPIVMKFLEEDIPVTDIVVMVQKEVADRM
                     NAIPGTKDYGALSIAVQYYCDTEIVAKAPRHMFIPQPNVDSTVIGLHVRDKRKYDVHN
                     EDIFFKTVKASFGQRRKTLLNSLGGLGFLNKDEIREVLKEANIDEKRRGETLSIEEFS
                     VLSNIINTKVSSK"
     gene            30150..31415
                     /locus_tag="KR250_000451"
     CDS             30150..31415
                     /locus_tag="KR250_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0066420.1"
                     /translation="MGSKRKFKRDYIILEAKDINFRYKERVLPKAFAKVEVNDEKAII
                     ALYVENLKYVKDGYKAVAIKDDHGLIDLGNIILNEQGRGEFVLDLEDYDDEIKGIALL
                     YERNIPLIGFKGNKLENYEDILFQEVEEEEYEEYEELEEEDEEIEDNEESDRDEGSEE
                     EYIIEEYDEDEYEDYWEDDEDIDEGELKARTEVEDSGDEEIEEFNNYKEIENYNRVKV
                     QEEYIDIEYDEEEEEEEEVFEVNREEDRFVSSKPTKSKKVQKDQNNIEKIKRANGVSQ
                     GESGNKQTSTLLMPRQIKKGLKLFKEVKPFVTDYIDKTRWWKIEINPTTLCGYTMPYL
                     GYLNVLNYTMYSDAVLHSYKYRHYLFGVQYDEYNKRKHYIYAIPGNRNEQPDNGSTGF
                     LRYQACDNRTNSLGYWLCFIDSRQRNIVR"
     gene            31566..32681
                     /locus_tag="KR250_000452"
     CDS             31566..32681
                     /locus_tag="KR250_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HBH0066421.1"
                     /translation="MINEQRILNEFLELVQIDSLSLKEGNVAKVLVKKLEEIGCSVVI
                     DNAGEKANGETGNIIATLKGNKEGKKILFSSHMDTVTPGIGVKPIVDEANGIIKSDGT
                     TILGSDDKAGIAAILEGLRYINENNIEHTDIQVVFSICEECGLVGAKNLDYGKIDSEY
                     AFILDSGGSPGEIIVKAPAQDVINVKILGKTAHAGLEPEAGISAIMVAARAIENMNLL
                     RIDEETTANIGIINGGTATNIVTGEVNIIAEARSLKENKLDVQTKHMVETFEKAAKDF
                     GAQIEIDVNRAYTPIDVSEDSEIIKLAKKAFSNLGIEGHTESTGGGSDTNILSKNGIE
                     AITLGIGMKNAHTLSEHIAIKDLYDSAKMVVEIIKEA"
     gene            32867..33763
                     /locus_tag="KR250_000453"
     CDS             32867..33763
                     /locus_tag="KR250_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBH0066422.1"
                     /translation="METRYEEANKITIQSILWNVVLTIIKVIAGVIGNSSAMIADGLH
                     SASDIISSIGVLIGNYVSSRPGDREHNYGHEKAETLVSFVLSILLIFVSITIGIEAIK
                     SLFNLDALSVPSILPLVVSVISILIKEYQYRITIKVAKKINSPALKADAWHHRSDALS
                     SVAAFIGIGGSILGFKPLDPIASVVVAIFVAKVGISILISSVNELMDVSVDEEEIKEL
                     KFIVADTEGVKNLGDIKTRKHGAMAYVDLTICVDENLTVKQGHDIATKLEKHIIKHME
                     FVKGITVHVEPCTNCQGNKCNN"
     gene            33833..34630
                     /locus_tag="KR250_000454"
     CDS             33833..34630
                     /locus_tag="KR250_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0066423.1"
                     /translation="MSKKRKLKKKAAILIGVVVFFIIYFISFRIGINMEKDKSEVPKK
                     VEKEEEDNRSLRTQIKEKKKVYISDKNIENIKIEEDMWDEIKLFFSDFKKVRNTTSSY
                     EAVYEGYSDDGVKFSTDLNFFRVYTVNKEEYYKVPVSSKDAFKKLLDESIYTSFDLLS
                     HYKDWKEVTITHGKEIKKVHRWKFDDLAHKMISKRVVGKVQPEKSKERSDYNFTINIK
                     GDNFDVKVDTMGKDYVKVTSKKNVSYYEVHTSLFDYLKNEIFEIGKK"
     gene            complement(34685..36037)
                     /locus_tag="KR250_000455"
     CDS             complement(34685..36037)
                     /locus_tag="KR250_000455"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HBH0066424.1"
                     /translation="MNIHFIGIGGISMSALAEICINKGYQVSGSDSNESYLLDKLRDQ
                     GATIYIGQKKDNISDDVNMVVYTAAVHPDNEELVAAKEKNKLVMNRATFLGQIMREYK
                     NSIAVSGTHGKTSTTSMLSTIFEYADLDPTILVGGNLSMIGGNVKIGNSNHFITEACE
                     YVDSFLNFNPKISIVLNVEEDHLDYFSGIDEIKASFNKFGKLLPPEGYFIINGDDENI
                     DDILYDVKATIIKYGRDSDNDAVIKDIHFDNSGHGIFRIEYEGRDLGEFELSVYGLHN
                     IYNASSAIMAALVSGIDLETIRKNIKIYKGVGRRFETKGYYKNALVVDDYAHHPTELK
                     ATLAAAKKLKKSTLWCIFQPHTYTRTKSLLGEFSEAFYAADKVIITDIYAAREKDPGD
                     IHSKDLVEKLYQNNVDAIYIKEFEDIVKYLRENVKDNDLVITAGAGPIYKVADLLVEK
                     "
     gene            36256..37134
                     /gene="purR"
                     /locus_tag="KR250_000456"
     CDS             36256..37134
                     /gene="purR"
                     /locus_tag="KR250_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HBH0066425.1"
                     /translation="MKFKRTERIGAIVKILSDNPNKIYTLSYFTNQFNAAKSTISEDL
                     LVVKNVFEKLHLGKVITISGAAGGVKYIPKTSIAENAEFLMELCERICDKSRILSGGF
                     LYLIDLIYDPTIAAKIGKIFASNIEYVDADYVVTMETKGIPMALMTAKAMNLPLVIIR
                     KDIKVSEGPTLSMTYVSGNSSKVESMSLPRKALKPDSKVIIIDDFMRGGGTIKGMVDL
                     MNEFGAEVIGTGVFISTTNPSEKMVKDYISLIQLDVNGDKIVVEPNLKTFKDEYRNED
                     LDEEEDSEFEFEIDED"
     gene            37290..37568
                     /gene="spoVG"
                     /locus_tag="KR250_000457"
     CDS             37290..37568
                     /gene="spoVG"
                     /locus_tag="KR250_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
                     /protein_id="HBH0066426.1"
                     /translation="MKITDVRVRKLTEEGKMKCIVSITFDNLFVVHDIKVIEGHNGLF
                     IAMPSRKVGEGNFRDIAHPINAEMRQVLEDAVLQAYHEALVQWEVAAE"
     gene            37781..39160
                     /gene="glmU"
                     /locus_tag="KR250_000458"
     CDS             37781..39160
                     /gene="glmU"
                     /locus_tag="KR250_000458"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HBH0066427.1"
                     /translation="MNFKAIILAAGKGTRMKSKYPKVIHKVCGKEMVNHIIDVSKKSG
                     VKDTVVILGHEADVVKEKLAEEIIIAMQTEQLGTGHAVKMAKEYINDEDTIVVLCGDT
                     PLIKEETLKRLFEYHIENKYHATVLTTRVGNPTGYGRIIRDKKGDLLKIVEQKDANSE
                     EKMISEINSGIYCFNGKSLREALDLLNNNNSQGEYYLTDTAKIMRDKGLKVGAFAGST
                     IEELMGVNSRVELSKAEEIMRRRINESHMVNGVTIIDTNSTYIESDVMIGNDTIIYPG
                     VMLQGKTRIGSDCIIGMNSSITNSEIGDGTEIKNSTIIDSKVGENSTVGPYAYLRPKS
                     DLGNNVKIGDFVEVKNAIIEDGSKASHLSYIGDAHVGKNVNIGCGVVFVNYDGKNKFK
                     SIVKDNAFIGSNSNLVAPVVVEEKGYIATGSTITHDVPDGALAIARERQVIKEGWVEK
                     KNQKDDQSK"
     gene            39255..40205
                     /locus_tag="KR250_000459"
     CDS             39255..40205
                     /locus_tag="KR250_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003361732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HBH0066428.1"
                     /translation="MNTSGSEIKIIAGNSSKELAQKIADYIGVSVLDCEVGTFSDGEI
                     CVNMNETVRGCDVFVVQSTNSPVNDNLMELLILIDALKRASAGRITAVIPYYGYARQD
                     RKAKARDPITAKLVANLITAAGADRVLTMDLHAAQIQGYFDIPLDHLLGGTILANYFN
                     EKKIEDLVVVSPDLGSVTRSRKFANTLNGEVPIAIIDKRRPKANVCEVMNLIGDVKGK
                     NVILLDDMIDTAGTIVNAANALKEFGAKDVYACCTHGVLSGPAIERIANSEISELIVL
                     DTIQLPEEKRIDKIKIKTVAPLFGDAIRMIFSNESVSKLF"
     regulatory      41045..41130
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            41292..41813
                     /locus_tag="KR250_000460"
     CDS             41292..41813
                     /locus_tag="KR250_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBH0066429.1"
                     /translation="MKLKKNKKGFTLVELLVVIAIIGILAVVAVPALFSNINKAKVAS
                     VESDYSSVKSAALSYYSDTNKIPVTTTDKAGLTILETYMESLPDKADIGGKYKLIKVG
                     NKLVLQIGTDTEGVTLTEAQSAKLLSDIGKDKIYTGVTGDNFGDELTDTTKIDNKALY
                     IVLIDNTVMDSTK"
     gene            41965..43641
                     /locus_tag="KR250_000461"
     CDS             41965..43641
                     /locus_tag="KR250_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HBH0066430.1"
                     /translation="MAKKVRIGDKLVEKGYITEEQLKWALSEQKNSGKRLGEFLVQEG
                     LIDSNLLISVLKELLDIESIFLEGTEIDTLATKMVPENICKRYTVFPFKIDGNKICLA
                     MSDPQDREAVQDVRRMSGKDVEIFISSTEDINKAIGHAYAHSEINKAMTEYNKNRTGG
                     VRETVILEEDVNAAPIVRLVNNILENAVRMEASDIHIEQSENYMRVRFRIDGMLREYM
                     RMNSAPYKAVISRIKIMSDINISEKRIPQDGRIYLKVDNKPIDFRVSTMPTNRDEKIA
                     MRVLDKSNFMVSKEVLGIDEHGSKIYDELINTPYGLILVVGPTGSGKTTTLYSMLNQL
                     NTENRNLLTIEDPIEYELPGVNQSQINEKAGLTFASGLRAFMRQDPDIIMVGEIRDTE
                     TAEIAIRASLTGHLVLSTLHANTAVGAISRLLDMDVESFLITSSVLGVISQRLTRKIC
                     EHCKVSYEADIGEKKALGIDVNESVTIYRGKGCERCNNTGYKGRLGIFEMLEITPEIK
                     ELIDSSANQREILKMARKQGMVSLKEDIVKKVLNGKTTVEEMIRIILMTD"
     gene            43653..44861
                     /locus_tag="KR250_000462"
     CDS             43653..44861
                     /locus_tag="KR250_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HBH0066431.1"
                     /translation="MKSFKYTVIKEDGKKQTDVIEANDFNEARNLLRKRNLRVIEIKE
                     SKNKNIRLSSRKRKKDLGADQISHFCRQFAIIVSSGINSISGLETLARRSTNITLREE
                     INRIVAEIKVGSTIADSMLSSKSKFPKLLGAMVATGEATGKLDEVLKSMASFYASEHR
                     VKQKLRNAATYPLIVLISSFIMIFIFTTFMVPKMINSITTVGATLPLITRIVMGFGMF
                     MKKFWIIVLLVIILFIYQFKKYLKTSVGRAHKDRVINKIPVLGKGINCMVATRFSRAL
                     YLFVSTGYPLVQGLDYIIDSVNNTMAEKILASAKDGITRGEGLAENLERYTYFDSVLV
                     QMIAIGEQTGELENISRQMAEFYEYESEIYLNRMASMIEPVLIIAVGIIVAILVVSIF
                     MPMLSIYDAM"
     gene            44876..46588
                     /locus_tag="KR250_000463"
     CDS             44876..46588
                     /locus_tag="KR250_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HBH0066432.1"
                     /translation="MNKVYVSYYGDSISIVEGRYKHDKDKFYVKNFNVFSIEDIAQDF
                     SKEKYALLRYALENVKLKSRHVVFCLNTRDVILKPQKLPNMDKKDLDGFMKLEMDEMM
                     ALESDEYVFSYEVSSETPDNSDGDESSLNLIIGAIQKEEVNAIVEIMHEFDLVLDRID
                     TLSTAYLRMLKNLDYNDIMVTNISDNSTIINIYKKDTLFISDNIPIKITAKDMDMQLL
                     NVVEESKGLMNYYSSRNFGKVTDTILILGKRYSNKLIYETFSSAFSSYVSQGLTEIMD
                     ISDMITGDIDEDDINSIVELLGSMIEPKGKRDFENINFLPMDILRKQIKAKQMKNILK
                     IAPIVILILAIPYLCLIFSNNLANKELENVNGEIRNIESAYYQIGNIEKMIQSKSEEI
                     KVYDMLIGKKVKWENLLDDIDKNTPSKVELTSLSTTYQNLGTNNKADNSKSNKTTTDK
                     SSNNETKSSTKSDSNGDKTDNDLNEDKTTNVSEALTGNSETNSNNTDKDDKTPLYDKI
                     PNVINIEGNAKDSSYVGQFLYKLKGLYYFKDVKLHSIKSNAEDSNYTFSMTLYLKEGV
                     LTNE"
     gene            46581..47489
                     /gene="pilO"
                     /locus_tag="KR250_000464"
     CDS             46581..47489
                     /gene="pilO"
                     /locus_tag="KR250_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HBH0066433.1"
                     /translation="MNKDIFKKDIKDIFKMEVSLKGILIKVIILLAILSYGYFGLYPQ
                     YQKYIATKNSVKQATEKLNIYKEKTALMPKYEKQLEEVESELKEKSKNLLYDMQDGLF
                     LIGLDKKMKAYNIDLTSYDVGNVKEYEHFYGISTTISVRGDYRNVKKLMAYLEEQKNV
                     TQILDYTMTSYIEPEKEEEETTNNKDLIKNVNINKDTKIYWVEGENKWHLYKDCPLIK
                     EGEIIKNDKLLDRSTNYLCNTCVLRAATEKEAKKEVVAPKAEGVVDATFKFIMYTKEN
                     TTMILDTDNPSSWRPGKYNPFKSSAK"
     gene            47516..48082
                     /locus_tag="KR250_000465"
     CDS             47516..48082
                     /locus_tag="KR250_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBH0066434.1"
                     /translation="MSLYKNNEKGLTLLEVIIAVFILTIVLSISYKVFNGITSAVKKQ
                     QIITDAQVNINLINKYLNRDLENCKELTKTGSGNNYEYNIEMPDNVVKYEVSIETKKN
                     TEVYSVTRIQNNTIDTENEVREEIIYNQPLVQNNKEMKETPFKIEKQTGKSIYTVSIY
                     YNESVQESHKNSNLNNKTYTFDVMSRIG"
     gene            48119..48646
                     /locus_tag="KR250_000466"
     CDS             48119..48646
                     /locus_tag="KR250_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBH0066435.1"
                     /translation="MSKKNSERGFSLIEVLVAMAIMGIVLFAFFNIINTNNKANTKND
                     TDITSLNYVQSEIENLREKIKSGEFDFDSLDKLEDGTVVYEKLIDKSKKVVYDKVLSE
                     GDVSLYDTPYEKITTIKDEDGNLIDKGNITNKIKTIVEDKSGQIYKIAVTGKSMNDYS
                     SKKEVKIVTEIFKDK"
     gene            48667..50205
                     /locus_tag="KR250_000467"
     CDS             48667..50205
                     /locus_tag="KR250_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0066436.1"
                     /translation="MRKWNKFKSERGAALVLVLVVVALLSIVGLIFSNQIANRIKSTK
                     TTNEGIQAKYLAETCVENSIDKAYEKLYDELEKMDNEFKSENQEKSISRSKLRNISDE
                     DFNNQDEENIEAERLGYMNNINFYLNKASSDLEKASMELKKLYDLDMLDYRDIEYVDA
                     NIISHRNSILEICKNYTSGDISKINEYILKEDIDSTTLIEAKLVNNDILLKKFLEENK
                     IENEHLNSAFSHTYKALDNISLAMQNMIEYRHTFHIDEPKVEVSNGIPDSQQYYELIQ
                     NPIINSMEYSWNSKWDTLENLLEILPNQTQGFNSLRVHLRNNVRKFEKLSDNISSGKK
                     NTAKNFLKYKELLYEISDQCNQLKSMSYEKIPVKYDNMALITTFDYIQNELLAEIKCR
                     LKELKPQEIDKTEGITIKIPFYKADYDMTKEGWPKLKENGSGAELSLMVTGDKNGIKE
                     VEVTDGKKNIIGLGVEENSNSKYKVDAIVNFNLNIDTNVVGNYDIKDKILINHDISSY
                     KKVN"
     gene            50305..51387
                     /locus_tag="KR250_000468"
     CDS             50305..51387
                     /locus_tag="KR250_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HBH0066437.1"
                     /translation="MYIYDLLEQGIRLNASDIHITVGTNPVARVKGGFVKLSEQILTS
                     EVTMQMAKDIAGESMFKVIEEHGEADFSASLKTGERFRVNAYRQKGNYAIAIRTITAE
                     IPTFEKLGLPESIKSFTEKHKGLVLVTGPTGSGKSTTLASMINIINEKQQKHIITLED
                     PIEYVHHHKQSLVNQREVGTDTESFHSALRAILRQDPDVILIGEMRDPETVSIALTAA
                     ETGHLVFSTLHTVGAAKTIDRIVDMFQPSQQQQIKTQLSTVCEGVVSQQLLPTADGKG
                     RIAAIELMFATPAIKNLIREGKTYQIPNMIQTGVKVGMKTMDQDLMELYKNGKITKDM
                     ALSRCTDQEFMTRMIGGVNYNGYYNR"
     gene            51368..52150
                     /locus_tag="KR250_000469"
     CDS             51368..52150
                     /locus_tag="KR250_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HBH0066438.1"
                     /translation="MDIIIVNLYVFIVGIVFGSFFNVCIFRIPEGISISRPPSHCMSC
                     NTRLKPIDLIPVLSYLMSGRKCRYCYEKISSRYAIVELITGLLFLTVFMLYGISTSTI
                     YYLVLVSLLIIITFIDIDHFIIPDKILVFGAIFSMIFNLLFKEIPVKDSILGVFICGG
                     SVWIIVLLIEFVIKKECMGGGDIKLFAMLGFYMGVKNGLLTALLSVYVGAVYGICVII
                     ISRIKGKEYNSVIPYGPFISIGALITILSGKQLLELYFSMVI"
     gene            52252..52902
                     /locus_tag="KR250_000470"
     CDS             52252..52902
                     /locus_tag="KR250_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HBH0066439.1"
                     /translation="MEYIIIISLFFAHLINKSLCVFCDREKKNLYNTFFIYIITVVLS
                     VLIYLKFGISVECVQYMVLIPFLIIISIVDYYTIYVYDITIVSGIIVQGIIFLAFFNI
                     VQFDYINHIFGLLIGFIIPYIIAKLTKGLGYGDVGLYSLCCFALGNNYGFYVIVLSFI
                     LAFLFFIILYITKIFKDVDIRKQIPFTPFISLSTILIIFTEYNIFNFYYNLLYKFI"
     gene            52916..53476
                     /locus_tag="KR250_000471"
     CDS             52916..53476
                     /locus_tag="KR250_000471"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HBH0066440.1"
                     /translation="MYVVVGLGNPGKKYEKTRHNVGFDVIDILAKEYNISVTKIKHKA
                     LIGEGRVGTEKVLLVKPQTYMNLSGETLIDIYKYYKVDLSNIVVVYDDIDLEVGKIRI
                     RKKGSGGTHNGMKSITKCLGSNDFPRVRVGVSKPEAGQDLADFVLSRFRKEESDNINE
                     ALEKAADAIDSIIRENIDMSMNKYNG"
     gene            53493..56879
                     /gene="mfd"
                     /locus_tag="KR250_000472"
     CDS             53493..56879
                     /gene="mfd"
                     /locus_tag="KR250_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HBH0066441.1"
                     /translation="MNDVFLYPLQNSKEYKDIINCIKNTKGSLLVNGLLPVQKPHISY
                     SIFNDLSRQMIFITSSDLEAKKVYEDLSFYMEDKVEYLGFQDIYFYHLDAKDRNEEAK
                     KLKVLLKLANKEKIILVTSIEAVLRKYIPKQVLLDSVSHYKVGDSLDLEKLTEKLVSL
                     GYERVSKIEGFGQFSIRGGIIDVFSLEYTNPIRMELFDDEIDSIRTFDVYSQKSIDKL
                     QQFSITPSREFIYPEKTTDALVKLKKETTKNTDEGTFQNIDYISSKTYFEGVENYIDY
                     IYPEENKSIFTYLADDAIVFINDITRLKERCENYINEFRENYKLNLERGLAIKNQGKL
                     LYHYTDLEYLVKDKSVVLNSLLPKSINNFSIKSIINFESREVPTFNGKVDLLVEELNR
                     LKYNGYKIILATNTLERANKLGKDLLDKGLETTISKDRDIEIKSSQVIIVPAHINSGF
                     QYKSIKFVVITDNEMIGVYKRASKTSNKKVKKGKKIESFLDLSVGDYVVHENSGVGRY
                     TGIEQITVNAIKKDYMKIVYQGGDNLYVPIDQMDKVQKYIGAEVEKVKLNKLGTNEWT
                     KAKAKVKKEIEDMTKDLIELYAKREKIQGYKFSKDTPWQAEFESLFPYQETEDQLKAI
                     EETKKDMESSKVMDRLVCGDVGYGKTEVAIRSIFKACMDQKQVAVLVPTTILAQQHYN
                     TFKERFENYPLRVEVLSRFKTPKQQKQIIEDAKKGLVDILIGTHRIISKDINLPNLGL
                     VVIDEEQRFGVKHKEALKKIKSTVDVLTLSATPIPRTLHMSLSGIRDMSVIEEPPQER
                     HPVITYVTESKESVIQDEIERELSRGGQVFFVYNRVEHIEEMASMIQKLVPDARVAVA
                     HGRMTSKSLENIILGFLNKDYDVLVCTTIIETGMDISNANTMIIYDADKMGLAQLYQL
                     RGRVGRSSRQGYAYLLYEKDKTLSEIAEKRLKAIREFTEFGSGFKIAMRDLEIRGAGN
                     ILGSQQHGHMAVIGYDLYVKMLNDAIKKVKGEPIVEEIDVEIDLSVNAYIPDNYIKDE
                     LIKIEMYKKIASIENKEDMLDIQEELEDRFSDIPKPVQTLLTIAYIKSLCKILKIEKI
                     RQLKDEILLVPITKYRTKQKIGYNIVTELEELLEKMCKVK"
     gene            56910..57905
                     /locus_tag="KR250_000473"
     CDS             56910..57905
                     /locus_tag="KR250_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBH0066442.1"
                     /translation="MKKVITLVIAMILVVSVTACSSSKGETVATVEGTKISSDEFKKT
                     IALYKDSMEQTYGKDIWDKEVEKGVKYKDKFKDLILDQLITTEVIYSQAKKDNLLPKK
                     EDVEKSFKELKDAMGKDEKYKEQLKKLGIDDEFLRDQQEKDLAMQNYQSNFAKKTKIS
                     DEEMKKYYDTHKDEFKKDEVEASHILLKTVDDNNKPLSDKEKAEAKKKAEEALKEVKS
                     GEDFAKVAKKYSQDTSASDGGKLGFFSRGQMVAEFEDAAFSMKKGEVSDLVETQYGYH
                     IIKVTDRINEQTSFEDAKETIKDQLLKNKYQEQIEKLTKEAKVEKDEKVINKITI"
     gene            58029..58568
                     /gene="spoVT"
                     /locus_tag="KR250_000474"
     CDS             58029..58568
                     /gene="spoVT"
                     /locus_tag="KR250_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein T"
                     /protein_id="HBH0066443.1"
                     /translation="MKATGIVRRIDDLGRVVIPKEIRKTLRIREGDPLEIFTAKDGEV
                     ILKKYSPIGELNEFSQEYAETLAEVLGYGVLITDLDSIIAVSKLPKKDYKEKSISDEL
                     EAIISERTVKYSKDKNILPLFKEDQKEYTSQVIMPIISASGDCIGSIVVVSKDKDTIA
                     ESEEKSLKIAANFLGKQVQ"
     gene            58688..60301
                     /locus_tag="KR250_000475"
     CDS             58688..60301
                     /locus_tag="KR250_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBH0066444.1"
                     /translation="MNNNKNKDSFLKGALILGLAGVIVKIMGGFFRIPLGNMIGSEGM
                     GYYQAAYPVYTLFLTLATAGFPTAVAKLVSEKVAIGNFKGANKIFKVSHTVLFITGII
                     SFCILFFGADYIVTNVMKNPGALYSMKAIAPALLFVPAMSAYRGYFQGRQDMTKIAVS
                     QVAEQFFRVVLGLTLAYLLMKSLGQTFGAAGAISGATIGSIASMLYLVFAYMLGRKER
                     RAEIDASQRFKDERVSYIFKKLLTVAIPITIGASVMPLVNMIDNVIVIRRLMEAGFTY
                     KVANSMFGQLTGMAMATINLPAVITTAMSMSLVPAISKAYALGNKSKARKDTKSAVKV
                     TLLIVLPCAFGMASLAIPIMGLLFPHEPSSVGTILFTLTPCVLFLGLIQTLTGIIQGM
                     GKPIVPVIALCVGMLCKIVISYTLTGIPDINVLGSAFGTVTAYFVAAMINLLYVKKHM
                     NVNFSKKEFIIKPFITVMTMFIMVKLSYGALVGFLGNSISTIIAICVGGIVYVVVILG
                     IGGIKKEEILTMPKGDKLYKLLKKVKLIR"
     gene            60408..61856
                     /gene="mazG"
                     /locus_tag="KR250_000476"
     CDS             60408..61856
                     /gene="mazG"
                     /locus_tag="KR250_000476"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HBH0066445.1"
                     /translation="MGKISIVGLGPGDYSLISQGVLESLINSPKVFFRTEKHPIIDKL
                     KETIQYTSLDYFYEKEENFDNVYLKISEFIIEESAKGDLVYAVPGHPRVAEKTVSIIE
                     RLANEKHIAVEILPSMSFVDAMFNYLAIDPSEGFKLVDAFEIENSYIDLDTSMIITQV
                     YDKFIASNVKLKLMEHYDYNQEVCIVNGAGVKDLESKKFIKLCELDRNENFFDYLTSL
                     YIPKSSKKMYNTVHDLEIIVNTLRSPSGCEWDKKQTHQSLKNSVIEEAYELCNAIDNN
                     DIDEMVEELGDVLLQVIFHCQIGNEEGYFDLKEVVNGICKKLIHRHPHIFSGAKLDMS
                     KFEKTWEELKKEEKGESTITDGLRRIPKHLPALMKASKVQHKAALVGFDWDNVEDVFK
                     KIEEEYQELLDEHRVGNIKYIKEELGDLLFSIVNLARFLDIDSGEALNCTTEKFINRF
                     DFIEKSAMNLNKKLEDMTLEEMDEFWNQAKKQ"
     gene            61945..62223
                     /locus_tag="KR250_000477"
     CDS             61945..62223
                     /locus_tag="KR250_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003153447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HBH0066446.1"
                     /translation="MNKAELVSKMAEKSGLTKKEAEAALNAFMSSVQDALVNNEKVQL
                     VGFGTFETRERAARQGRNPRDPEQVIDIPASKAPVFKAGKGLKDIING"
     gene            62295..62537
                     /locus_tag="KR250_000478"
     CDS             62295..62537
                     /locus_tag="KR250_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HBH0066447.1"
                     /translation="MRLDKFLKVSRIIKRRTVAKDACDKGIVTINGKVAKSSSEVNIG
                     DTLEIQFGEKKMKFKINEVKEHVLKNDAKEMYKILE"
     gene            62599..62853
                     /locus_tag="KR250_000479"
     CDS             62599..62853
                     /locus_tag="KR250_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YabP"
                     /protein_id="HBH0066448.1"
                     /translation="MEQNITLKDRSKLVISGVEHIYSFNDKRVELKTSVGEMVIEGEN
                     LDMSKLSIDENIISIDGTINSMVYAKPKKPQESFLKKVFR"
     gene            62854..63387
                     /locus_tag="KR250_000480"
     CDS             62854..63387
                     /locus_tag="KR250_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0066449.1"
                     /translation="MIPFTQDVSIFYATIYGGILIGVLFDFYRGLRGNFKFINYFAII
                     FDVLFWFLATVIIFVTINLTEFFDLRYYHFVALFIGFILYYNTISKIVLSIINKIIRF
                     VRNSFKKVTHYIVSFLNNLYYVIIYSLHLLFDIIFYIPNIFIATRKSIKRKSNKKLKN
                     KKKSKPKKKKNKTKKRV"
     gene            63393..63677
                     /locus_tag="KR250_000481"
     CDS             63393..63677
                     /locus_tag="KR250_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HBH0066450.1"
                     /translation="MNLRKKFSGQIIVISLFLGISIFSMMTGFVFEYTKAKEYKKEIA
                     SLNKQLKKTEIQINSLKKDEKSYEGDLEDIARKRLNMVKPNETVYVDINR"
     gene            63790..64710
                     /locus_tag="KR250_000482"
     CDS             63790..64710
                     /locus_tag="KR250_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA family phosphatase"
                     /protein_id="HBH0066451.1"
                     /translation="MKVGAIDIGTNSMRLLIADYRDGNFINRKKFVNTTRIGYGVDSK
                     GYIKEDAIKRNIKALKEFSDKCNDEKCEKIYCMGTSALRDSKNGDYFVKLAKEETGID
                     VDIVTGIEESNLGFKGVLEGIQGDDTIIVIDIGGGSTEFIVGDSEGIKFNKSENVGAL
                     RMTERFLKHDIIEDEEFEAMSSFIYDEIDKTLNHIKDRGLKTLIGIGGTITSLSAMNQ
                     ELEIYSMEKIHNSKIHKKEIEEILQKLIKMTLSDKKNLKGLQEKRADIITAGVKILNI
                     IMEKLEIEEITVSEYDNLEGLICQKSKKMY"
     gene            64800..67172
                     /gene="spoIIE"
                     /locus_tag="KR250_000483"
     CDS             64800..67172
                     /gene="spoIIE"
                     /locus_tag="KR250_000483"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein E"
                     /protein_id="HBH0066452.1"
                     /translation="MQRSVAVVKNKTVKPNVIKLISGYIDFTTAAFAVMGFLLSRSII
                     VDSVAPLGIAFFICASKIDKYKIPVFISTLLGILLSSNDFGNVAKYAVCLTIFMAMNK
                     KLKLSDSIAKVAFIGAIILLPISMGQAFFSNRYVYGLLIAGVEAIIMFISIYVFSYGI
                     GLMANVNNRMSVRTEEAISISLLLTFSIMGIGDISLFGISIRTILSTVLILLTSIVGG
                     ATMGASSGVIIGVASILNNMTSAVYMGIYSFSGLISGAFNKINKYFCILGYILSWTII
                     YLYTSGITSNMMQLRDILLGCLIVLVLPERLFNKIEKLIKSNVASNEIVYDYIMRSKN
                     LTNSRLNSIYKTYDDLADTFDKIREKDKVLDQRDIANVIDMIHNDECKSCSMRRMCWE
                     SRFNHTYTMIYNILEKIEEKGELSLNDIPKNFRKECMKPESIVKISNHYYKMFVLDYD
                     WSVKFSESRKLIANQIRSISKSIKSLSQDLEGDIMLDIEKEKNIYEQLERYDITVDKV
                     SYLTKSNSEFEISIEKKTCHDGCMCEDKIVNIISDLVGENMSVRKIGCHCLGGKCKAT
                     FVKSQKYKAVTEVSAMSRDGHILCGDNYTYMEINDGKYMMAISDGMGKGKKAYEESSA
                     TIDILEKMIDAKIKDEIVIDTINNMLLLKSSEEMFSTLDLGILDLKRGCLETIKMGAC
                     STYIKREDGEVDLISSSSLPVGILSDVKIDRKNVKVKEGDYVIMVSDGIVDAGRNNNL
                     GDNWLIYFLKNIETTNPKEISNLILDRALELQALQIEDDMTVLVTKICTK"
     gene            67523..69415
                     /gene="pepF"
                     /locus_tag="KR250_000484"
     CDS             67523..69415
                     /gene="pepF"
                     /locus_tag="KR250_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HBH0066453.1"
                     /translation="MNRKLCKAIIPLFISIFCMLIISIKADQGYQKNNDRAITVMNNN
                     DTEDDAEKKQEIDYEKTCWNLQSLFKSDDQWKKELKSFDKDTKELKNYIGKVTKSPTH
                     LSFALNIKEKLDIRLNKLSAYVKLKQDTNKNSYKYLDMNDSMSKSYGNYMGICSDLEL
                     EILKLSNKDYKKIISNKNINRKYGAYLRDIRRNKVHYLDDKSEDILSKVSSISSLPSQ
                     VYELFRNMDRKTNLTPAQYASELESSDRENRKKAYQDELITYNDNINTIAGLITGQVK
                     KNIFYSQERGYESSLEMYLEADNVDEKVYNNLIRTVNSNMDSLHKYIDLRKKVLKLDK
                     VHSYDMFVPMVKAVDNNINYEKAQSIVYSALSPLGKEYNDVVYKAFNEKWIDVYSHEN
                     KVSGGYCLSVYENHPYILLNYNNSLGSVSTLSHELGHAVYEYLSSKNQNYFNSNPSIF
                     THEVASTTNEALLYEMLIKEAKGNDEKAYYITQYLDLIKDTLYTQTMYAEFEKTIHEL
                     VEKGKSVNALVLNDVWGQLLKKYYGQSYELDQLSKVGWARIPHFYNSFYVYKYATGCS
                     AGVSFAQDILKNGSDNYMSFLKKGSSDYPINLLKSSGINLTNTKPIENTIKKFDSLVA
                     ELEKLL"
     gene            69529..70494
                     /gene="galU"
                     /locus_tag="KR250_000485"
     CDS             69529..70494
                     /gene="galU"
                     /locus_tag="KR250_000485"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HBH0066454.1"
                     /translation="MQVKVKKAVIPAAGLGTRFLPATKAQPKEMLPIVDKPTLQYIIE
                     EAVASGIEEILIITGRNKKSIEDHFDKSVELELDLEKKGKKELLEVVQNISNMINIHY
                     IRQKEPKGLGDAIYCARHFIGDEPFAVMLGDDIVDNDVPCLKQLTDAYEEYRTTILGV
                     QKVNQEDTNKYGIIEAKNIEGRVYKVKDMVEKPESGKAPSNIAILGRYIITPEIFDIL
                     KDLPPGKGGEVQLTDALKILSKKEAMYAYNFEGKRYDVGDKLGFLEATVDFALKKEDL
                     KEDFIKYLKHVCSEFDKNNNVLYNNTDEKIDLIEVNEARELEIQK"
     gene            70994..72598
                     /gene="rho"
                     /locus_tag="KR250_000486"
     CDS             70994..72598
                     /gene="rho"
                     /locus_tag="KR250_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HBH0066455.1"
                     /translation="MRRIFLILDDKVLEGLGSKTLVELREIAKELKIKSITTYKKNEL
                     IEIINSKSKNEEKVNEETKEKNEDIKEDIKNRKENIERESIEKEVEYKSPTKSYNKKE
                     IDTQNISQNQEHRNQRNDYNKTTSVNDSNKSSNISNRMVRNNNKNYYMPKQVDESKIV
                     DEFNTSKEDEVVGVLEILPDGFGFLRGSNYLSTEGDVYVSPSQIRRFNMKTGDKIKGI
                     TRHPKSGEKFRALLYVQKINDENPDTAIQRNAFETLTPIFPEERLTLETNRNEIATRI
                     IDLISPIGKGQRGLIVAPPKAGKTVLLKSVANSIAKNHPNVELIVLLIDERPEEVTDM
                     KESIEGDVIYSTFDQVSSHHVKVAEMVLNRAQRLVEHGKDVVILLDSITRLARAYNLT
                     ISPTGRTLSGGIDPGALHGPKKFFGAARNIRQGGSLTILGTALVETGSRMDDVIFEEF
                     KGTGNMELHLDRKLAEKRIFPAIDIYKSGTRRDDLLLDDEEKTALWRLRREMSNNSVM
                     EITDKVIELIKRTKDNKEFVKSIKNL"
     gene            72751..72951
                     /gene="rpmE"
                     /locus_tag="KR250_000487"
     CDS             72751..72951
                     /gene="rpmE"
                     /locus_tag="KR250_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018591942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HBH0066456.1"
                     /translation="MQKEIQPKYNPVEVRCACGNTFVAGSTKDEIKVEICSECHPFYT
                     GKQKNIEKGGRIDKFKKRFKMD"
     gene            73096..73989
                     /locus_tag="KR250_000488"
     CDS             73096..73989
                     /locus_tag="KR250_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HBH0066457.1"
                     /translation="MAKQAVGGQAVIEGVMMQSKDKRAVAVRKSDGEIALKEDRIKSW
                     VRDKNIDKIPFVRGSFVMIDTMIQGIKSLNFSSEFFMEEAEEDKFDLFIKKIFKDKAN
                     DIIIIFSLVIAMLLSAGLFIFIPTLVGGAFSKVMPNDFMLNLIEGIIRIAILFAYIVL
                     ISRSKDIERVFQYHGAEHKSIYCYENDLELTVENARKFKRLHPRCGTNFLFIVMAVSI
                     ILFAFFGWPNPILRIFMRIICVPIVAGLSYEVIRVLGKYDNGFTKIIAYPGMMLQYFT
                     TKEPDDEQLEVALEALKAVVD"
     gene            74007..74855
                     /gene="prmC"
                     /locus_tag="KR250_000489"
     CDS             74007..74855
                     /gene="prmC"
                     /locus_tag="KR250_000489"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HBH0066458.1"
                     /translation="MTIKDIIIKYSDKLKDISDTPRLDTELLLQKTLGVDRLYIHLNL
                     NKELTEEQKTKFMGFAEERLNGRPIAYIVENREFMGLDFFVKEGVLIPRPDTETLVEE
                     IIEICREKKDVSILDIGTGSGAITISLAKYIENSKIMSFDISETALEIAKKNAIINEV
                     GEKIKYINSDLFTAISDSNIKFDIIVSNPPYIKKQDIETLHKQVKDYEPYNALEGGED
                     GLDFYRRITEQGKKYLNKCGILAYEVGHNQAEDVINIMKSNGYKKIYTKKDIQGIDRV
                     VIGYNI"
     gene            74899..75963
                     /gene="prfA"
                     /locus_tag="KR250_000490"
     CDS             74899..75963
                     /gene="prfA"
                     /locus_tag="KR250_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HBH0066459.1"
                     /translation="MLKKLEVLEDTYKDLSEKIGDPDVINDQKVWQKYIKEHADLEPI
                     VMKYREYKSVLDSIKESKEILQEESDEELRELAKMELAEMEEKVAPLEEEIKILLLPK
                     DPNDDKNVIVEIRGGAGGDEAALFAGDLFRMYSRYAERRRWKIELLSASDTGVGGYKE
                     VSFMIKGKGAYSRLKYESGVHRVQRIPSTESGGRIHTSTSTVAVLPEVEDVEVEINPN
                     DLRIDVFRSSGNGGQSVNTTDSAVRVTHIPTGEVVSCQDGKSQLKNKEQALKILKARL
                     YDKALAEQHKDIAAERKSQVGTGDRSERIRTYNFPQGRISDHRINLTLYKLDAFLDGD
                     IDEMIDALITVDQTEKMTAI"
     gene            76092..76772
                     /locus_tag="KR250_000491"
     CDS             76092..76772
                     /locus_tag="KR250_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HBH0066460.1"
                     /translation="MILKVTIIGLLAGVIGTGLGGVISAIFKREVDKYLSFFMGLSGG
                     IMLAVVVFDLMKESMDKMGIINTVIFTFVGALITMYIKTKLDVSGNMASGYLIFISIL
                     LHNLPEGLAIGSSFMSTESLGITLAIVIGLHNIPEGLAMALGLVCNKMKLSKVILFTV
                     IAGLPMGLGSFLGVYFGGVFTSLIGVFLATAGGTMMYVVLEEIFPHSKSVYCIIGFLL
                     GTMIVNYI"
     gene            76801..77841
                     /locus_tag="KR250_000492"
     CDS             76801..77841
                     /locus_tag="KR250_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HBH0066461.1"
                     /translation="MKTIISNIDINNIDYEEIKIQAKLLREGKTVIFPTETVYGLGAN
                     ALDENAVKKIYEAKGRPSDNPLIVHIYEKEEVYDLAKDISDKAKLVIEKLWPGPITII
                     LNKKDIIPYKTSGGLDTVAIRMPSNVIARAIIKEAGIPIAAPSANISGRPSPTKAKHV
                     YEEMNGRVDGIVLGGDSNFGLESTVLDLTEETPMILRPGSITKEVLEELLGEVKLDPS
                     LSKKEDNQKAKAPGMKYKHYSPNADVYIISGKRENVATKINDMIKSNRDKGLKTGVMC
                     ISRNREFYDGEVIDLGNSLEEVASNLFDALIEMDKKGVDVIYSEEFPKTGVGQAIMNR
                     LLKSAGYKVIKA"
     gene            77858..78307
                     /locus_tag="KR250_000493"
     CDS             77858..78307
                     /locus_tag="KR250_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight protein arginine
                     phosphatase"
                     /protein_id="HBH0066462.1"
                     /translation="MNILIVCTGNTCRSPMAEAILRKAIKESGRSIEEYSISSAGIST
                     ANGMGASENSIEVLKEIGIDLSNHRSKVITKKLIDESDIILTMTKSHKEILVQAVPKC
                     KEKVYTFKGFANKNEEDISDPFGGNLDIYRSTMREIMYSVNEIVKKI"
     gene            78324..78776
                     /gene="rpiB"
                     /locus_tag="KR250_000494"
     CDS             78324..78776
                     /gene="rpiB"
                     /locus_tag="KR250_000494"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HBH0066463.1"
                     /translation="MKIGLGCDHGGYNLKKEIISYLESKGIECVDYGTNNATDSVDYP
                     VYGEIVANSVINKEVDYGILCCGTGIGISLAANKVPGIRCAVVSDVFSAKMSKAHNDA
                     NMLSLGERVLGKGLALEIVEAWINTDFEGDRHARRVNMIKSIEEKHNK"
     gene            78812..79441
                     /gene="upp"
                     /locus_tag="KR250_000495"
     CDS             78812..79441
                     /gene="upp"
                     /locus_tag="KR250_000495"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HBH0066464.1"
                     /translation="MSKVVETNHPLIQHKLTLMRDKNTGSKDFRELLTEIAMLMGYEI
                     TKDIPLKDVEIETPIQKTSSKVVAGKKLAIIPILRAGLGMVDGLVSLMPAAKVGHVGL
                     YRDPETLKPVEYYCKLPQDIGERDIIVVDPMLATGGSAVAAIDLLKSKGAKSIKLANL
                     VAAPEGIAEVQKYHDDVDIYVASVDERLNEHGYIIPGLGDAGDRLFGTK"
     gene            79742..80557
                     /locus_tag="KR250_000496"
     CDS             79742..80557
                     /locus_tag="KR250_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF2563 family selenium-dependent molybdenum
                     hydroxylase system protein"
                     /protein_id="HBH0066465.1"
                     /translation="MKNCIVVRGAGDLATAVIHKLHNSGFKVVALEIEEPLAIRRQIS
                     FCEAVYEKKVTVEGVECIFCNTEKEIEEVLSQHKVALIIDPKGEKLSKLNPIVLVDGI
                     LAKKNLGTTKDMAPLTIALGPGFCAGEDVDVVIETMRGHNLGRIITNGRAEKNTGVPG
                     AIKGFSKERVMHSQYDGIMENVCKIGDIVEKGDLLCYINNYEKKHEVRATISGVLRGL
                     LKDGAKVNKNLKILDIDPRKEEVKNAFTISDKARCIAGGVLEAILNNGIYPQN"
     gene            80783..81220
                     /locus_tag="KR250_000497"
     CDS             80783..81220
                     /locus_tag="KR250_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase"
                     /protein_id="HBH0066466.1"
                     /translation="MRPSWDEYFMEIAEVVKKRSTCIRRQVGAVIVRDKQILTTGYNG
                     SPRNLEHCENIGCKRQELNIPSGERHELCRALHAEQNAIIQAAHNGISVDGATLYVTT
                     RPCVLCAKMCINAGILKIVYEGDYPDDMSTELLKEAGIELIKF"
     gene            81405..81632
                     /locus_tag="KR250_000498"
     CDS             81405..81632
                     /locus_tag="KR250_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HBH0066467.1"
                     /translation="MSKRNTHTEIAKLFSLISQIGLMIVISILGCTFLGKFLDSKLNT
                     TPVLTIIFLLLGVGGAFSGVYKTLIVYTRRK"
     gene            81637..82023
                     /locus_tag="KR250_000499"
     CDS             81637..82023
                     /locus_tag="KR250_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HBH0066468.1"
                     /translation="MDIKLLNQVRSVTKGIVIFDVVLIVLMLITSTLSKPVLLGLIFG
                     SIIAMLNFRLLAISLEKSVTFPAGKAQAYASAQYAVRLFIMAVVLIASATVPHLNIIG
                     TALGLLSTKFVILSKNFINKLKRKEA"
     gene            82026..82730
                     /gene="atpB"
                     /locus_tag="KR250_000500"
     CDS             82026..82730
                     /gene="atpB"
                     /locus_tag="KR250_000500"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HBH0066469.1"
                     /translation="MNQAHYYLIGNFKISETILVSWGIMAFLAIASYFLTRNLKKVPT
                     SKVQIFLEFAVGGLAKMVTDVMGKNTVKRMPYMIPYIGSLFLFFACSNLSPLLGLRSP
                     TTDLDTTAAWAMITFFMIYFAGVKFKGLSYFKELAQPLAIMLPINIIGELARPISLSF
                     RPFGNILGGTIIMGLFYQLLGFISSLIPGLSIPIGEILIPVPLHLYFDLFAGLLQAYI
                     FIMLTMVFISSAGELD"
     gene            82799..83059
                     /gene="atpE"
                     /locus_tag="KR250_000501"
     CDS             82799..83059
                     /gene="atpE"
                     /locus_tag="KR250_000501"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HBH0066470.1"
                     /translation="METAIVAAASAIGAGIAVATGIGAGIGQGIAAAKAAEAVGNQPE
                     AKGDITSTLLLGVAIAESSAIYGLVISIILLFVNPFFKYLGM"
     gene            83182..83679
                     /gene="atpF"
                     /locus_tag="KR250_000502"
     CDS             83182..83679
                     /gene="atpF"
                     /locus_tag="KR250_000502"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HBH0066471.1"
                     /translation="MEKALVGITWEFVFQIVNTFIIFLLLRKLLFKPVLNIIESREND
                     IKSDLVEGEKAKNEGLALKKEYESKINFAKDEGQEIIKQATIRAEQKSDDIVNTAKKD
                     ALDIKEKANKDIEQERQKVINEIKNDISNIALLAASKVIEKDLDKSKHEELIENFIKE
                     VGEAK"
     gene            83676..84221
                     /locus_tag="KR250_000503"
     CDS             83676..84221
                     /locus_tag="KR250_000503"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HBH0066472.1"
                     /translation="MINVIANRYAEALFQLGEEENSTDVLFKELEKVVDMMTKVSKDF
                     YKVLKSPLVSKSEKKNLVEIIFSKEVSSNIKNFLKVLVDKDRISYLEDIELAYKELLN
                     KKNNVIDGVAISAIPMSETDIKELEVKLSNKYNKNVTIENVVDKTILGGVLVRIGNEQ
                     IDGTVKTRLDKMKEKLSEVIS"
     gene            84238..85740
                     /locus_tag="KR250_000504"
     CDS             84238..85740
                     /locus_tag="KR250_000504"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003405060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HBH0066473.1"
                     /translation="MNLKPEEISSIIKQQIKNYENKVELTDTGSVLTVGDGIASVYGL
                     EKAMSGELLEFPGEIYGMALNLEEEVVGAVILGDDSEIKEGDIVKRTGRIVEVPVGEA
                     LIGRVVNSLGQPIDGKGPIAYTKTRPVESEAPGIIDRRSVYEPLQTGIKSIDSMIPIG
                     RGQRELIIGDRQTGKTSIVIDTILNQKGKDVICIYVAIGQKRSTIAQLVSSLEKGGAL
                     DYTIVVSATASESAPLQYIAPYAGAAMGEEFMYNGKHVLIVYDDLSKQAVAYREMSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDELGGGSMTALPIIETQAGDVSAYIPTNV
                     ISITDGQIYLQPELFYSGVRPAVDPGISVSRVGGSAQIKAMKKVAGTLKLAYSQYREL
                     AAFSQFGSDLDEDTKKRLAQGERIVEILKQGEHQPIKVENQVMIIYAVINNHLEDIPI
                     DNIARFESELYAFVDNNYPEISRKILGGEDFTHDLTDAINEFKEKFVVEV"
     gene            85768..86646
                     /gene="atpG"
                     /locus_tag="KR250_000505"
     CDS             85768..86646
                     /gene="atpG"
                     /locus_tag="KR250_000505"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HBH0066474.1"
                     /translation="MAGAGIKAIKTRMGTVESTKQITKAMELVASSKLRKAKEKAEDS
                     REYFNTLYNTVKDIAQTTRGIRNEFLKEREVKNKCYVVVAGDRGLAGGYNSNVLKESV
                     SHMKGKKETVITVGKKAHEFFSKRNYNIAKDINSVEACGYEDATEISQTIIDLYKKGE
                     VDEVYIAYTKFKSALSQEVEILKLLPLVFEKDTDEQGMAVKEEKQNRSKVQYIPSAEA
                     VLSYIVPQFISGTVYGGILESFASEQGARRTAMESATDNANEMLSNLELSYNRARQSA
                     VTQEITEIVGGVEALK"
     gene            86659..88053
                     /gene="atpD"
                     /locus_tag="KR250_000506"
     CDS             86659..88053
                     /gene="atpD"
                     /locus_tag="KR250_000506"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HBH0066475.1"
                     /translation="MANVGKVVQIVGAVLDVKFDSEQSLPNLLNALVIKLGDKEIVAE
                     VAQHIGDDTVRCIAMSATDGLVRGMEVVDTGGPISVPVGDETLGRIFNVLGKPVDGKP
                     APKSAPKLPIHRPAPAYDELETTAEILETGIKVVDLLAPYLKGGKIGLFGGAGVGKTV
                     LIQELINNIAKQHGGISVFSGVGERTREGNDLYGEMSESGVINKTALVFGQMNEPPGA
                     RMRVALTGLTMAEHFRDEQGQDVLLFVDNIFRFTQAGSEVSALLGRMPSAVGYQPTLA
                     TEMGALQERITSTKKGSITSVQAVYVPADDLTDPAPATTFSHLDAKTVLSRQISSLGI
                     YPAVDPLESTSRILDPSIVGKEHYEVARGVQSILQRYKELQDIIAILGMDELSDEDKL
                     IVARARKIQRFLSQSFTVAEQFTGNPGQYVPVKETVRGFKEILEGKHDDLPESAFLFV
                     GTIEDAVRKAKGSM"
     gene            88056..88316
                     /gene="atpC"
                     /locus_tag="KR250_000507"
     CDS             88056..88316
                     /gene="atpC"
                     /locus_tag="KR250_000507"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HBH0066476.1"
                     /translation="MASEFSLKVVTPSNTFYDGDVEMIVVRTTKGDKGVLKGHIPFVS
                     GIPVGTLKIKKEGSFKEAKIAEGFINVTQDKTVILTESAEWI"
     gene            88778..89158
                     /locus_tag="KR250_000508"
     CDS             88778..89158
                     /locus_tag="KR250_000508"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HBH0066477.1"
                     /translation="MNIFDIGVDIIEIDRIRKAVDKNNRFLEKIFTDREIEYFNSKNF
                     KAESIAGNFAAKEAISKSIGTGIRVFNFKDIEVLRDEMGKPIVKTYNNLAKMCIDYNV
                     LEIKVSISHSKDYAIANAITIIKD"
     gene            89173..89634
                     /locus_tag="KR250_000509"
     CDS             89173..89634
                     /locus_tag="KR250_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBH0066478.1"
                     /translation="MMDKTAKEIMTTDVIVAKQDDSIADVANMLIAEKIGGLPVVDSE
                     NRVVGIISETDILKKEKYIEAPLYINLLQGLIFLDDLKKVEKDIKQVAAYKVGELMSK
                     DIIKVHEDDKFDDVANVMIKKSINRVPVVDDDNKLKGIICRYDIIKALYNE"
     gene            89655..90245
                     /locus_tag="KR250_000510"
     CDS             89655..90245
                     /locus_tag="KR250_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0066479.1"
                     /translation="MRKKWTIVCIMFLALVIIVIGCQKRQSTKEEVYKDFQKQISDMN
                     YYSCKAEVEVVGNKSPHNYVLIHTYKKTDNYKLEVISPKHLKGKSIEYQGDKILVKNP
                     KISDVVELPNTGKNNQYLFVGDFIKNYLQNEEMKVKLSKGHLVLETFIPGDNKYFNKQ
                     VLYVNADTKNPEKMEVLDKEGVPRFTVKYKDFEYRN"
     gene            90260..91417
                     /gene="alr"
                     /locus_tag="KR250_000511"
     CDS             90260..91417
                     /gene="alr"
                     /locus_tag="KR250_000511"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HBH0066480.1"
                     /translation="MQKITVPTWAEINLDNLRFNLNNIKNLLEEDIKICGVIKADAYG
                     HGAVEVAKLLEKEKVDYLAVARTAEGIELRQNGITLPILNLGYTPDEAFEDSIKNKIT
                     MTVYSLETAQKINEIAKSLGEKACVHVKIDSGMTRIGFQPNEESVQEIIELNKLEYID
                     LEGMFTHFATADEVSKEYTYKQANNYKFMSDKLDEAGVKIAIKHVSNSAAIMDCPDLR
                     LNMVRAGIILYGHYPSDDVFKDRLELRPAMKLKSKIGHIKQVEPGVGISYGLKYTTTG
                     KETIATVPIGYADGFTRIQKNPKVLIKGEVFDVVGRICMDQIMVRIDKDIDIKVGDEV
                     ILFGEGEVTAERIAKDLGTINYEVLCMISRRVDRVYMENNELVQINSYLLK"
     gene            91450..91737
                     /locus_tag="KR250_000512"
     CDS             91450..91737
                     /locus_tag="KR250_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HBH0066481.1"
                     /translation="MYDKSKEKIEVTLPDSLISEVDSIVQMENSNREDFAKAAFQFYI
                     TQKKKIHIKESMKTGYREMGQINLSLAELGMTVEEVSSDDKNEGKIAQGEY"
     gene            91706..92089
                     /locus_tag="KR250_000513"
     CDS             91706..92089
                     /locus_tag="KR250_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBH0066482.1"
                     /translation="MKGRLPKVSTNCEIKRGDLYYADLSPVVGSEQGGVRPVLIIQND
                     VGNKYSPTVIVSAITSQINKAKLPTHVEISSNEYGLNKDSVILLEQIRTIDKKRLREK
                     IGCLDENMMIKVDDGLQISLGLFTF"
     gene            92260..93087
                     /locus_tag="KR250_000514"
     CDS             92260..93087
                     /locus_tag="KR250_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HBH0066483.1"
                     /translation="MRDHKGLNEIARIIRRDIVSMIHRAKSGHPGGSLSVVEILTALY
                     FDEMNVDSSNPKMEDRDRFVLSKGHAAPALYATLAEKGYFDKEELNGLRKIGRMLQGH
                     PDMKGTPGVEISTGSLGQGFSVACGMAMASKLDNAPWNVYTLLGDGEVQEGIVWEAAM
                     SAAHYKLDNLIAFLDNNGLQIDGDIESVMSLGSIVDKFKAFGWNVIEIDGHDFDQIFA
                     ALDIAKSTVKKPTMIVAKTIKGKGISFMENQAGWHGTAPSDEELEKALLELGGADNE"
     gene            93080..94021
                     /locus_tag="KR250_000515"
     CDS             93080..94021
                     /locus_tag="KR250_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HBH0066484.1"
                     /translation="MSKMATREAYGKALVKLGQINDNVVVLDADLSKSTKTHDFYKSF
                     PDRFFNMGIAEQNLIGAACGLSTAGKIPFASTFAMFATGRAFEIIRNSVCYPKLNVKI
                     CATHAGLTVGEDGASHESVEDIAIMRAIPNMTVLVPADGVETEKIIFEIAKYNGPVYV
                     RLGRSSVPVLFDEDYKFEIGKGTVLREGKDVSIIACGIMVNEALLAQEKLQEEGISAR
                     VINMSSIKPIDKDLILESAKETNAIVTVEEHSIIGGLGSAVSEVVGESCPTIVKKVGI
                     KDTFGESGTPNELLKKYELTCDDIIKTVKEAIIAKRM"
     gene            94444..95538
                     /locus_tag="KR250_000516"
     CDS             94444..95538
                     /locus_tag="KR250_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0066485.1"
                     /translation="MSEKVNVKTVISFAGAYVATVIGSGFATGQEIMQFFSFYGFAGI
                     IGGIISMVLFSWMGASVMSKGKELQLKEPIKIYRVYCGKYLGIFFEWFGPLFLFGVFV
                     VMISGAGATLTEYYGLNPFVGRVGMAVVALLTVSLGLDKLSKILGGIGPIIIIFTLLV
                     GGISLAKNIGNIGEAAQVLSTVKIAKPVPNAYLSGVIYTTYNTIVVMAFLTGLGASAA
                     NKKEAICGGILGGVALMAAAIMMHLAILSDIGNLYSKAIPSLFLADKISPVIGVLFSI
                     ILILGIYTTAVPLLWSVTNRFVDDKHPKFKLITLIAAILGLIGGFLPFDKLVGILYPY
                     TGYMGVIILVCVLYRQITKTSGYQEGISDK"
     gene            95764..96180
                     /locus_tag="KR250_000517"
     CDS             95764..96180
                     /locus_tag="KR250_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0066486.1"
                     /translation="MNVKFNIKGIIYGVIALVFVIGGFIFIPSMFVEKSKPIDYTVLQ
                     RNSIPEKILDVMDKYTNEERALAAKIDNKIYIIVTRGNNKYGIEMDKIESVVEEGKDV
                     LRVKIKYKDKEDSFPYIVVETNMSELPDRIELNATK"
     gene            complement(96362..96931)
                     /locus_tag="KR250_000518"
     CDS             complement(96362..96931)
                     /locus_tag="KR250_000518"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HBH0066487.1"
                     /translation="MKKEFRKKVIESRKNKNANFVLSNSNLITEKLLQMEDIRNATNI
                     MLYLNFNNEVQTDNLIKKLLSLKKIVSSPITIKENHTLIPTQITDLKEGLRIGAYGIR
                     EPNEKSPTIDIKALDVVIVPAVAYDIHCYRLGYGGGFYDRFLENLRKDAITIGIAFDF
                     QIFDSIPKETHDAQLDYIVTETRIITPNS"
     gene            97085..98242
                     /locus_tag="KR250_000519"
     CDS             97085..98242
                     /locus_tag="KR250_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HBH0066488.1"
                     /translation="MYLVISKKKAIFLGVILVIITAMVTSAFQLTLGNKVVISKELYE
                     DYKKYDKLLGLESIIKQDFYKKVSDTDLVNGAAKGLFLGTNDKYSGYYTKDEMENLIN
                     DSEGSYVGVGMYIGASKDGGLVVVPMKDSPAEKAGVKSGDKLVKVNGKSVSYKNSDEA
                     VRMMKGKKGKTVELTILREDKQLNFKVKTDQIIEKSIESKVIDNDLGYIEITQFISST
                     YTDFDKALKELKAKNIKGLVIDLRNNPGGMLDICKEVADELIGEGTIVYTKDNKGNTE
                     YLKSDKEKLGLPIVVLTNGESASAAEILTAAIVDNKEGISVGTTTFGKGLVQSVVRLK
                     DGTGYKLTTAQYFTPNGDYINEKGIKPTIEEKDENKQLDVATKWLREQIDK"
BASE COUNT        39479 a        10631 c        18094 g        30372 t
ORIGIN      
        1 ttttttgtca aaaaaaacaa aaaatacgta tttctttttt catttcggga aaactaaaca
       61 caggaattat ggactttata aaaatttaat tcttgaggag tgatataagt gaaaaaagtt
      121 gctttaattt tagctataat aggtgcgctt aactggggtg caattggtat tctaggtaca
      181 gacttaatag gtagtatctt tggtggaaca tatgaaatgg taagtagaat aatatacttc
      241 attgtaggtt tagctggatt atacttaata ccaagtttaa tgtctgatga tagagaataa
      301 tttatgcttt tatgttaaat acctcattct aaatgtttag aatgaggtat ttaattttat
      361 aaaaatatta atttgtttct tttttaatgg taaaatagat aagaattgaa gttttaaaaa
      421 gaagtattat gtaaaaaagg agtactatta atatatgttt attattaatg aattaaataa
      481 gattgtaaat gaagaagtaa tatcttttca tatgcctggt cataaaaagg gacaggttta
      541 taaaaagtta ggatataaag atatactaaa aaatttgtat aaaatggata caactgagat
      601 attaggaaca gataatttac actttccgga aggaattata aaaaattcac aaaaaaaagc
      661 atcaagagta tttaaaagtg attatacttg ctatttagta aatggtagta gctgtggaat
      721 acaatctgca ataatgtcag tatgtagtcc taaaagtaaa ataataacaa atagagactg
      781 tcatcagtct gttataaatg gatgtatttt aggagatata gatatagagt atgtgccttg
      841 tgagataagt gaagagacaa atatcttaaa agggatatct ataagtaatg tgattgatat
      901 aatagataaa aacttggatg caaaagcagt actgcttact tatcctacat attatggaat
      961 gacatatgat ttagaataca tatgtaatta tgcacatagc aaaaagatgg tagtgatagt
     1021 agatgaagca catggtgcgc atttagggct tagtgagagg cttcctaaaa cagctcttga
     1081 gcaaggtgca gatatagttg tacagagtac acataaaact cttccagcat ttacacaatc
     1141 gtcaatgata catatacagg gagaccgagt tgatatagat aaaatattga acatgttaag
     1201 aattacagaa tcttcaagcc cttcatattt attattatct tctctggaat tagcagtaga
     1261 tatatatgaa aataaaggaa aagaattaat ggaagagctt ttaaataata ttataacttt
     1321 caaaaataaa gttgattcaa atattaatat atacgacaca aatgataaaa caaaaatatt
     1381 tatatcatct aaaaacatgg gtttaacagg ttatgaacta gaaaatattt tgagaaataa
     1441 atataacata caagtggagt tatctaatta ttatggagtt cttttaatat gtacaatagg
     1501 aaatactgct gaggatttct ctagtttaga aacagcatta aacaatgttg taaaggaaga
     1561 ctttaaattt gaaaagttag aaaataaaaa ataccctgtt gttattccaa agaaaatatt
     1621 aacacctaga gaagcatttt ataaaactaa aaaaagtgtt aaaatatatg acagtatagg
     1681 taaagtttgt ggtgaatgta tagttccata cccacctgga ataagtgtaa tatctcctgg
     1741 tgaagtaata agtaaagaaa taatagacta tctaatgttt tgtcattcta aaggcatgat
     1801 tgtgagtgga ttgaaagatg tagatttaaa ttttatacaa gtaatagatt tagaagacat
     1861 ttaaaaatta tgatataaaa gtagatagaa gtagatagtt taaaattgtg aagagaaata
     1921 aatagactgc ttatatagag atatctaata aaagtactaa aaaagtaaaa agtgtttgta
     1981 tggaggatat gaaaatgaaa ggtaagttaa taattataga gagtggttca gatgcaagtg
     2041 gaaaggctac tcagacagaa atattatatg aaagattaaa aaaagaaggc tatatgatta
     2101 aaaaggtaga atatccaaat tatgaatctc catcatctgc cttggtgaaa atgtacctaa
     2161 aaggagactt tggtaaaaat gcatcagatg tagacccata tatagcatca acttttttta
     2221 cagctgatag gtatgcatca tttaaaaccg attgggagga gttttacaat gaaggtggaa
     2281 taattatcgc agatagatat acaacctcta atatggtaca tcaggcctct aaaatggata
     2341 tttgtgatag agaagagtat ttaaattggt tagctgatta tgaatttaat ttatttaaga
     2401 taccacaacc tgattgtgtt atatttttag atgtgcctat tgaatttagt aaaaagctta
     2461 tggagaatag aaaaaataaa attacagggg aagaaaaaaa ggatattcat gaaagtgaca
     2521 tagagtattt agagaaatct tacaacaatg ctttatatat agcagataaa tatgattgga
     2581 aaaaaataaa ttgtgtttta gataacaaat taagaagtat tgaaagtata cataatgaaa
     2641 tttataaaat agttcttgat tcaataaaac ctatggagag ataaatatgt attttgaaaa
     2701 tattataggg caagattttg caaagaaata tttaacaaat tctataaaga aaaataaact
     2761 taataatgcg tatatgtttg aaggaatgga tggaatagga aaaaagaaat ttgcagatga
     2821 attatcaaaa ctattacttg attatgaaaa tctcgaaaat agtccagact atgtactcat
     2881 aaaaccagat ggaaatagta taaaaatagc tcaaatcaga aatttacaat cagatatagt
     2941 aataagacct cataaagact ataaaatata tataatcaat aatgctgaaa aaatgactgt
     3001 ggaagctcaa aatgcacttt taaagacgct agaggaacct ccaaactatg ctataataat
     3061 cttagttaca aacaacaagg aatctttatt agaaactata aaatctagat gtgatataat
     3121 aaagttctca cctataccaa ttgaagactt aaaacgttat ttgataaata caggtataga
     3181 agaagaaaga gctcaattat tggctatttt ttcgagagga agtattgaga atgctctaaa
     3241 tctatctcag tcttctgagt tttcaatgat gagagaagat atacaacagt atattcagat
     3301 aatgttagat aaaaacatag tggagatttt aaatatacca aataatatgg aaaaatatag
     3361 aggtaagatt atagctttat tagatatgat gataaactat tttcgagata tcatcctatt
     3421 aaaagaaaat gtaaacaaga acatgttaat aaatgtagat aagctagttt tcattcaaaa
     3481 tatgaatgga aaaattagtt attctcaatt gtccaagatt attgatataa tagaagatgc
     3541 aaagagtaaa ataaaaagca attgcaattt caatataagc atacaagtta tgtctttaaa
     3601 tatatacgag gtgattaaat gataaagata gtaggtataa ggtttaaaag tgctgggaaa
     3661 atatattatt ttgaccctgt tgattttaat atagagcaag atatggatgt agttgtagaa
     3721 actgcaagag gcttggagta tgggaaagta gtcgttggtc caaaagatat ggatgaaagc
     3781 gaattagttt ctcctttgaa gccaattata agaatagcta cagaagaaga taaaaaagta
     3841 tatctagaaa ataaggaaaa ggcaaaagaa acttttgagt tgtgccaaca aaaaataaaa
     3901 gaacatgagt taacaatgtt tttaatagat tgtgagtata catttgatag gaataagttg
     3961 attttttact ttacagcaga aggaagaata gattttagag aattggtaaa agacttagct
     4021 gctatattta aaactagaat agaacttaga caaataggtg tcagagatga agctaaatct
     4081 ataggaggtc tagggccttg tggtagaaaa ctttgttgtt catcttggtt aggtgatttt
     4141 caaccagttt caataaaaat ggcaaaagac caaagtctat ctctaaatcc aacaaaaata
     4201 tctggaatat gtgggagatt attctgttgc ttaaaatatg agcatgatgt atatagtgaa
     4261 gcaatagaaa aaatgcctgt tgtaggtgca ttggtgcaat cagatgatgg aaaaggaaaa
     4321 gttgtagaag tgaatccttt attagaacaa attaaagtgg aatttcaaga caagacagta
     4381 aagatataca caagagaaga tataaaaata cttagagagc ctaaaaaatg tgatggctgt
     4441 ggtggtaaat gtaaggataa tgatggatta gatgaagcca cactaagaga gctaaagaaa
     4501 ttagaagatt aaatttaagg tagggctttt cctacctttt ttaaagcaat taatgcttaa
     4561 agttattaat atttgaggag aatttatata tgcaattaaa agattcagaa agaatagatg
     4621 atttacaatt aaaaggcctt aaaattatac aagatactaa tggtttttgt tttggtatag
     4681 atgcagtttt attagccaat tttactaaaa taaaaaaaga tgccaaagta gtagatttgg
     4741 gtactggaac tggcattata cctattttga tagctggaaa aagtgaagct aaaaaaatta
     4801 ttggcgtgga aatacaagaa gatgtatatg aaatggcaac tcgttctata aaattaaatg
     4861 gattagaaga aagagttgag attataaatg aagatataaa atacatagac aaagtcttag
     4921 acataaatgg ttaccatgta gtcacatcaa atcctccata tatgcatatt gatggaataa
     4981 aaaatccaaa tgataaaaaa gcaatatcaa gacatgaagt aaggtgcaat ttggaagatg
     5041 taattagagc tgcatccaga ttggttatgc ctagaggaaa attcttcatg atacatagac
     5101 caacaagatt ggttgatata ataactttag ggagaaaata taaattagaa ccaaaggtta
     5161 ttcaatttgt gcatccaaga cctcaaaaag caccgaactt agttttggtt caatttgtaa
     5221 aagatggaag gccagagctt aaaatattgg acccattgta tgtttatgga gaagatggga
     5281 attatacaaa agaattaaaa gcaatatata ataataaaga tataggagag ttgtaaatga
     5341 gcgggaaatt gtacatatgt ccaacaccaa taggaaacct agaagatata acatatagaa
     5401 ctttaagaat attaaaagaa gtagacttga ttgcagcaga agatacaaga catagtataa
     5461 aacttttaaa tcattttgaa atctcaaaac cactaactag ttatcatgaa cataataagg
     5521 attcaaaggg agattatttg ataaataaac taattgatgg tgaaaatata gctctgataa
     5581 gtgatgcagg tatgcctggt atatctgacc caggagaaga gataataaaa caagctatac
     5641 aaaataacat agagatagaa gtattgccag gtgcaacagc ctttgtaacg gctttagttg
     5701 gctctggtat ggacacacat agatttattt ttgagggctt tttagataga gataaaaaag
     5761 ttaggaaagc acaattggaa gaattaaagg aagaaagtag aactataatt ttctatgaat
     5821 caccacatag attgaaggat acactaaaag atatgcttaa aattcttggt aataggaata
     5881 tttctataaa tagagaactt acaaaaaaat atcaagaaat aataagagaa aatatagaaa
     5941 ctaccataaa aatatttgag gaaaaagaag taagaggaga gtttgtatta atagtagagg
     6001 gttttcatgg tgagaagtca aagaaagaaa attatgatgg gcttacagat agggaatatg
     6061 ttttaaaact aattgaaagt ggtatgaaca aaaaagatgc aataaaaata gtttgtaaag
     6121 atagaaagtt aaagaaagat gtggtttata aacaagtttt agatttataa aacctgaatc
     6181 tgaggtgatt ttatggaaat agggaaaatt ataatagaga gtttaaagag ggaagataca
     6241 cataaaatat ttatagaatt agataataag catgaattag aaaaaataat tcctaaaata
     6301 agctgtatga agtctgtagg tgaatgtaaa tatcatgtag taaattgttt tgaacattct
     6361 ataaatgcat taaaagaatt agaaatagta ttaaatgata aggatttttt tccaatacat
     6421 ctaagaaaac atgtgtcaaa ctatttaaaa acatatattg aagaaggaat aaataagtta
     6481 catgtattaa agctgggaac atttttacat gatataggta agcctgacag tatgacctta
     6541 gatgaaaccg gaagagttca tttcacaaat catgaaaaaa taggtgctca aataattgac
     6601 aatatgggaa taaagttaga gttatcaaca gagacatata gacttataag taaatatgta
     6661 agatatcata tgatactttt gtctctatat aagaaaaatg atttgagtag gaaagaatta
     6721 attaatgtat ttaacttagt agatgaggac actataggtg taataatgct tgggtatgca
     6781 gatatagtgt ctacagtaaa attattgggc aagagtggag aagtcagtgt gttaaagaca
     6841 tatatggaat atattttaac aaattacttg tataagagta attatacaca tgtataaatt
     6901 tagttaatat aattgagttt tagatgatta attgaagttt gaaatatacg gatttcaaaa
     6961 acataaaaga agttgtcttt gttgtaaaat tatgaaagac aacttttttt atatcaaaaa
     7021 ttgaaatttt attaaggata cttaatggtc tattaattaa taaaccatta gtatttaatt
     7081 tatattacaa tattgatttt gcttgtattg gcgttgatag tataacagag gtcttagtag
     7141 aaccaatttt ctttatagta tcaatcacat tttctagctc gtacatatct tttacaatta
     7201 cttttaaaag taagcaatca tcacctgtta tatggtgaca ttctacaatc ctagggtcct
     7261 ttgcagctga ctcaataaat tctgtatatc cattgcttgg aagagaaata tgaataaatg
     7321 cctttataac tctgcctaat gaatctgggt tgacaatagc tttatatcct tctataacac
     7381 cagactcttc taatcttttg actctttctg aaactgcagg agaagttaaa ccaactattt
     7441 ttcctaagtc cttcatagaa attctcccat catcctgtaa aatttctatg attctgtaat
     7501 ctgtaacatc cataaataaa cagtccccct taaataaaaa taattaatgc tataaaactt
     7561 ttttagaatc tatcatttgt gtttttaaat accaaagatt tctagataaa tcaaccttat
     7621 aaatgtgtgg ttttgaaagt cttttgtatt gttcccagat agtacttaat tcatattgta
     7681 catgattttt tatttcaaga acagcttttt tattatattt gcatctgcct tttatataca
     7741 atggtttaag aagctcttta actttgtaat ttgtaaatac cttcgttttc catgtataag
     7801 taggatggaa gatttctaaa ggtttagact catctataac ttcatcatgt aacataatca
     7861 aatctgcctc tgccttattg tcttcgttat atattcttat aactttcttg taaccaggat
     7921 tatttatttt ttctggattc tcagaaatct ttatcttagg ttcaagaact cctttttcac
     7981 aagatgcagc taatttataa actcctccaa gagaaggtga atcgaaagat gtaatgagct
     8041 ttgtacctac tccccatgaa tttatagtag ctccttctgc tttaagtgat gttatggtat
     8101 attcgtctag gtcattagat gcagttatgc tgatatctga aaagccagca ttatctaatt
     8161 gttttttagc ttctttagat aaataagcta aatctccaga gtcaagtctt atacccatag
     8221 gtttgtatcc tttttcagaa atatttttga atacttttat tgcattagga actccactgt
     8281 ttaatacatc ataagtatct acaagtagta ggcatttatc tggataaaca tcagcatatg
     8341 cttggaatgc ttcaagttca ttatcaaatt tttgtaccca actgtgagct tgtgtaccaa
     8401 ttataggtat atcaaacatt ttccctgcta gtacgtttgc agttccagca caaccaccaa
     8461 ctacggcagc cctagcccca tatatccctg catctggacc ttgtgcacga cgtaatccaa
     8521 attcaaatac agggtctcct tgagctgcaa agcaaactct agatgcttta gtagcaataa
     8581 gagactgaaa attaactatt gttaacaatg ctgtttcaat aagttgagct tgatataaag
     8641 gtgccttaac agtaattaat ggctcattag ggaacatgat agttccttcc tcaacagcat
     8701 atatatctcc tgtgaattta aattctttta aaaattttaa gaacttgtca gaaaataaat
     8761 ttaagttttt taagtactct aaatcttcat ctgaaaaatg aagattatct atgtattcaa
     8821 caacctgatc aataccacaa actatagtat accctccatc acaagcgttt tttctaaaaa
     8881 acatatcaaa tacaacaatg tcttcatgaa tattcttttc aaaatagcca tttaacatag
     8941 taagctgata taaatcagta agaagtgtta gatttctcat tatttctctc cttaattatt
     9001 attaaatatt aaaaattgta aaaactccca cacttatact aaaacataat atagtacaac
     9061 tttcaagtat aaagtattac ctaactatca taaaattatt taaagaggag gatgattata
     9121 ataggaaaaa tatggtttta tatggtatcg ataggaataa taggaagctt gatttctaaa
     9181 aatatgggtg aattaaataa agttatatta agtgaaacat caaaaggtat agagtttgca
     9241 ataagtctag ctggtatcat ggcactctgg atggggataa tgaatatcgc aaaagaatca
     9301 ggtttaattg aaaagatagg tcaaaaacta aatcctataa tgagaagatt atttccttct
     9361 attccaaaag gtcataaagc aatgtcttat atagttatga atattgcttt aaatatgttg
     9421 ggtgctggaa atggtgcaac agcatttggt ttaaaagcaa tgagtgagct tcaaactttg
     9481 aataacaaga aagatacagc cactaatgat atgattatgt ttatggtaat caatatatca
     9541 tctatacaaa taattccatt tactataatc aaactaagga tggatatggg ggctcaaaat
     9601 ccaagtgaaa taatttttac gactttattt gcaaccataa tatctacagc agtagcaata
     9661 ataacatgta aattttttca aagaagatat agataaaggg gatgtagata catggagata
     9721 ttttcaaact tacttatacc aataataatt ctgtacatag tagtttatgg taaatataaa
     9781 aaaatagatg tgtatgatag ttttgtaaaa ggagcaattg atgggttaaa agcagcatgg
     9841 gatatcctac catatataat aggtatattt ttggcaatag gaatattcaa aactggtaaa
     9901 ggtcttgata tgttagaatg gctatttaca ccaatagcaa atatgatgag tataccaaaa
     9961 gaattaatag gtctaataag tgtaaaacct ttatctggaa gtggtgcttt agggatgtac
    10021 agtgaacttg caaatagagt tggaatagga agtttagtag aaaaaatggg tgctacgatt
    10081 gtaggttctt ctgagactat tttttacaca atggcaatat actatggaag tctgaagata
    10141 aaaaatacta gacatacatt atcatgtgct atgattagcc atgttgcagg tgtcatagca
    10201 gctgttttta tttgctatgt aatatttgta taatttgttg acaatatggg aaaaatttta
    10261 taatatagtg attatataga aacaaatagt aatacaaatt acaagctatg aaaagaagag
    10321 taaataaagg ttttttctat cagagagcta ggttaggtga aagctagtgt caaaagcttt
    10381 attgaagatg gtcttggagc tttttatctg agcaagtgtg ctagggtaaa acggttggac
    10441 acgttatagt ctactaaggt atctataaaa tgtttctaca tttcatgtga aacataacta
    10501 ttatagatga attagggtgg taccgtgaat aatctcgccc ctatgttaac tcataggagg
    10561 cgatttttta ttatttaaat gaaagaatca tatgattacg aacgataaaa aattaggagg
    10621 taaataacat gagcaaaccg agtttttatg taacaacacc aatatactat ccaagtggag
    10681 gattacacat aggacatact tattcaacag tagctgcaga tacaatagct agattcaagc
    10741 gtttctgtgg atatgatgta aagttcttaa caggaacaga tgagcatggt gaaaaaattc
    10801 aaaagaaagc catagaacaa ggaatgtcag agatagagta tcttgatgga atgataaaag
    10861 atataaaggc cttatggaat actatggata tatcttatga tgattttata agaactacag
    10921 aaaaaagaca tacagacata atacaaaaga tatttactaa actatatgag caaggtgata
    10981 tatataaagg tgaatatgaa ggtagatact gtactccttg tgagagtttc tggacagaat
    11041 ctcaattact agaaggaaat aaatgccctg actgtggaag agaaacatat ttagtaaaag
    11101 aagaatctta tttctttaga ttatctaaat atgaagacag attaaaagag ttatttaaag
    11161 atgatagttt ctgttttcct gctgctagaa aaaatgaaat ggtggcaaac tttttagaca
    11221 aaggattaga agatttaagc gtaactagaa cgacttttga ctggggtata aaagttcctt
    11281 ttgatgaaaa acatgtaatt tatgtgtggg tagatgcttt atgtaactat ataacagcat
    11341 taggttatat gactgataat gatgaagaat ttaaaaaata ttggcctgcc aatgttcaaa
    11401 tagtaggtaa agaaatagtg agattccata caataatatg gccagcgctt ttaatggctt
    11461 taggtctaga agttccaaaa caagtatttg gacatggatg gatactattt gctgatgata
    11521 aaatgagtaa atcaaaaggt aatgtggttt atccagaacc tataatagaa agatatggaa
    11581 tagacacact taagtacttc ttattaagag agtttgcatt tgggcaagat ggaagttata
    11641 ctcatagaaa ttttgtaacg agaataaatt atgatttagc aaatgattta ggaaacttaa
    11701 taagtagaac tgtagctatg gttgaaaaat acaataatgg tataatacca acagcaaaag
    11761 tttcaactaa ttttgatgct gacttaaaag aacaagctgt ttcaactaga gaaaactttg
    11821 aagctgaaat ggataaaatg caattccatg aagcattaga gagtgtatgg aaattagtta
    11881 gaagaactaa caaatatata gatgaaacta tgccttgggc actagctaaa gatgaaacta
    11941 agaaagatga attagacaca gttttatata atttatgtga atctataaga ataatcgcaa
    12001 cactaataaa tcctataatg aatgagactg ctaataaaat atatgaacat ataggaataa
    12061 aaggtcaaga tgatataact acatgggaaa gtacaaaaac atttggtctt attggagaaa
    12121 atgtaaaagt atttaaaggt gaacctttat tcccaagatt agatgtagaa aaagagatag
    12181 aagaattaac taaaatgttc tctgggaaac ctccagtaga agaaaaacct ttagagcata
    12241 aagaagaaat tactattgat gatttagaca aaatagagct tagagtaggg aaaataataa
    12301 gctgtgaaaa acatccaaaa gcgaataaat tattagtatc acaagttaag attggaccag
    12361 aaacaagaca aatagtatca ggaatagctg aatactataa gccagaggat ttagtaggaa
    12421 aagaggttac agtggtatgt aacttaaaac cagttaaatt aagaggtgta gaatctcaag
    12481 gtatgatatt agcagcaggt gatgatggag acccatatgt tttaccattt acacaagggg
    12541 ctaaagatgg atgtgaggtt cgttagtagg taattaaata gtaaggagat taaagatgtt
    12601 atttgactca catgcacatt taaatgatga aagttttgat gaagatagag atgaacttat
    12661 tggttcatta aaagataaag gtgtagattt ggttgtaaat ccaggagcag atatagagac
    12721 gtcgataaca gcaattgaac ttgcgaaaaa atacgacttt atatattcag cagttggggt
    12781 acatccacat gatgtatcaa aactagatga tacagcaata gaaactctaa gaaaacttgc
    12841 aactgagaat gaaaaagttg tagctattgg agagattgga ttagattatt attatgatta
    12901 ttctccaaga gaagaacaaa aagagtggtt taagaagcaa attgaattag ctaatgagct
    12961 aaaacttcca ataataatac atgatagaga tgcacatgga gatacttttg agataataaa
    13021 gaatactaaa aatcctgaaa taggatgtgt acttcattgt tatagtggga atgtagagtt
    13081 agctagagaa tatgttaaga tgggttgtta tatatcaatt ccaggtacgg ttacttttaa
    13141 gaataataaa aaaactagag aagtagtaag agaaatacct ctagaaagat tattcataga
    13201 gactgattca ccatatatgt caccagagcc acatagagga aagagaaaca atccttctca
    13261 agtatctttt gttgctgata agatagccca ggaaaaaggg atatcttatg aagaagtatg
    13321 tagagttaca aaagaaaatg caaagaaatt ttttaatata aaatagacaa attaaatctt
    13381 ataatgatat atataataag tcattataag tataggatga gagtgcctca aaatgagttt
    13441 tttagtttat gaggcactct ttttgtatga aatcaaatat attttcatca tttcaacaat
    13501 gaaaaagaag tttatcatat tttgagatag tttgttttta tttatttaat tcatatttta
    13561 aaatatattt tcttaacatt gttaaaaaag aatttacaag atattcatta gaattaactc
    13621 aactctaata ttcacttaac ttaagttgtt tattatatta ttaaagagaa gtaaacgaaa
    13681 agtagattaa ggaggttgaa ttttgtatac agttgatagt actaagatat tgataaatat
    13741 gaacttagag aaactagaaa agatatactc aatgataaaa gatacatcaa gtatttctat
    13801 aattaaagaa cctaacttgg ctacagtaat ggtaagagct aatgaaagtg taaaaaacac
    13861 aacatttaat ttaggagaga tattagttac tgaatgtagt gtaaaggtag atgaaagctt
    13921 gggttacgga atagtttcag aaaacaataa taagaaagcc atttatttag cagttataga
    13981 tgcagtttta catagcaata acagtaaatt tgatgagtta aagaattaca taaataaaag
    14041 tgtatatgaa gagaacttaa ggtatgaaca agagattatt gatgaattta gcttgattaa
    14101 taaaacgaaa gttcaattca atgctatgga ttaaatagga ggagaatcat ggtaaattat
    14161 acacaagaaa tatatagaaa gatgctagat agtgcttcat ttcctgggaa attaaataat
    14221 atagataata taaatataga aaataataca aaactattga atggagcaat tggtatagca
    14281 atcactttat tagaccaaga agttaccttc tatatagaaa attatactca aaaagatata
    14341 aaaaatataa gagcacttac aatgtcaaat caaatagatt atagaaaatc agattatata
    14401 tttttagata ttaattcaga agtggatata ttacagatta aaataggttc ttttgaatat
    14461 cctgatgaaa gtgcaactat agtacatcaa gtaaatgatt tatcaacgta ctctcaaact
    14521 aagtatataa agcttgattt gagtggtcct ggtataaaat ctaaaaatag tatatatata
    14581 aatggaatta gagaagaatt tatagaaaga ttatctagca taaataaaga ttatccaata
    14641 ggaatagatt tgattttagt agataaaaaa ggagaaattg catttatacc aaggtcatca
    14701 aaattaagtt gggaggtatt atagaaatgg cttatgtagc agttaaaggt ggagaagaag
    14761 ccatattaca ggctaaaaat atacttgagt tttatagggt aaaaggagat agcttgccta
    14821 tagatgtaaa gcagattgag gaacaaatgt cttatgctgt agacaaagta atgtctgaag
    14881 gaagtttata caacaaggaa ctatcagcat tggctataaa acaagctcaa ggtgatatat
    14941 tagaagcctc tttctatgta agagctttaa ggtcaacaat gcctagaatt acatattcag
    15001 aacccataaa tacagaagaa atgagaataa aaagaagaat atcagctact tttaaagata
    15061 taccaggagg tcagtatctt ggggcaacta gagactatga aaatagaata ttgaatgtag
    15121 aattattaga agaagatgaa aaaaagaata gacattttag agatagtttt ataaatgaat
    15181 tagaaacagt agatgaaatc cctgaattta caaaaatatc taatatattt agaaaagaaa
    15241 atttattaca agaagaagtt tgtgaaaaca aattggaaga agttattgat gatattacaa
    15301 gaaatagttt aagatttcca gtttctaggt cagcaaagtt acaggcatta actagaggtg
    15361 aaagtggagt agtgtcaggt tttgcttatg ctattgtaag aggttttggt gatgaacatc
    15421 catatataag tgaacttaga actggtaatg tatttataaa agtaaaacat cccatctatg
    15481 aagatgaaga aataataata ggggatatgc tggttactga atgtgaaata gtttcaaaag
    15541 catcacaaaa taagactgaa aattctaaaa ctaattcgga agataaaaat aagatgtctt
    15601 taggatatgg tctttgtatg gggaataatg agaataaggc tatatctatg gccatactag
    15661 acaaatcttt ggagccaaat agtgaaaaac tagcacaaga tgaagaattt gtattgcttc
    15721 atatagatgg aatagattca ttaggattta tatctcactt taagcttcct cactatgtag
    15781 attttaaagc ggatgtagaa agaataaaat agtttgagga ggtaagttta ggtgatatat
    15841 aactatggtt ttttagacga aaacacaaaa aaagaaataa gaagaaagat attaaaagca
    15901 atatcaatac ctggttatca agttcccttt ggttcaagag agttgcctat agctaaggga
    15961 tggggtactg gaggtttaca attaacacta tcattaattg gtaaaaatga tgtagtaaaa
    16021 gtaattgacc aaggaagtga tgattctaca aatgcttgta atataaggag ctttattgca
    16081 agtgtaagta atgtagaaac cacaaaagat acattagaag ctacattgat tcaaacgaga
    16141 catagaatac cagaagaaaa acttaaatct aatcaaatac taatatttca agtaccaata
    16201 ccagaaaccc ttagaatagt agaacctagt gaagtagaaa caagaagaat gcattcagaa
    16261 gaagattata gtaggatgtg ggtatatctt tatgaagata tagttagatt taatgatata
    16321 tctatagcag ttgaatatcc atgtaaagta aatgatagat atttaatgaa tccatcacct
    16381 atacctagat ttgatataaa aaaattaaat atgtcagaca atttattctt gtttggtgca
    16441 ggtagagaaa aaagaattta tgctattcct ccatatacaa aggtagaacc tttagagttt
    16501 gaggattata agtttgaaga ggaaaagttt gaaggaaaat actgtagttt atgtaacagt
    16561 acaaatacat ttttagatga agtatatgac agtgacacta atgaaaagta ctatagttgc
    16621 tcagatacaa gttattgtga aaaagttaga ttaaaaaata acagtataga tgtgacaata
    16681 ggaggtactt ggaatgagta aaataaatga ctctaaaaga ttagaaaata tagacaagca
    16741 agtattaata ggaagaaata tcaatcttat ttatggagat gggtgtgaaa agtgttttga
    16801 aagcacagga tatgaaagca ataatatatg taagtattgt ggttctatag ttgcatgtaa
    16861 taatgtaaat ttagaacttt atgaaggaga agtccttgga attgtaggtg aatctggttc
    16921 aggaaaaagc actttattaa aaatactgtt tttccaagaa catgcaaata gtggagaagt
    16981 atatatatcc cattacaata aagacacaaa tatactagag ctttcagacc aaaaaaagag
    17041 atacataaag aatcatttta tgggtattgt gtatcaaaat ccccaccttg gtttaaattt
    17101 agactttagt agtggtggta atatagcaga aaaattattg atggcaaatt tatataatgt
    17161 tgaaaaaata agagatagag gcaaagaact tttaataaaa actaatatac ctgtagaaag
    17221 aatagaccat aaaccaaaat actttagtgg gggaatgcaa caacgagttc aaatagcaaa
    17281 agctctttca aataaccctc caatattgct attagatgag gttacaacag gtttagatgt
    17341 ttctgtgcaa gcggaagtat tggatttaat aagagaaatc caaagagaac ttaagatttc
    17401 tatgatagtt gtatcccatg actttgatgt tataaagatg cttgctgata ggacaattgt
    17461 aatgaaaaat ggaagtgtaa ttgagtctgg tcttacagac caaataatgg aggacccaca
    17521 acacccatat actcagcaac ttataaattc attgctataa atatcgtttg taaataagca
    17581 aaatgggggt ttttaataaa atgaaagaaa ctatacttga agtaaataat tttagtaaag
    17641 actttgagtt acatgcattg aacaagacta taacggcatg tagtaatata agttttgaag
    17701 tttcaaaagg tgagttttta ggaataatag gtaaatctgg agcaggtaag tctaccatat
    17761 taaaaagtat ctataaaaca tatataccaa caactggaga aataattttt aattcagaaa
    17821 tatatggtaa tgtagacctg gctaaaattg gagaccgaga aatgataaat cttagaaaga
    17881 aagaaatagg atatgtatca cagtttttaa agacacttcc acgaataact gcaattgaac
    17941 tggtagtaca ttctttaatt gagagtgggt ttgaaaaaga ttgttcgtat gatatggcaa
    18001 aagatatact taaccaattt gaaataaagg aaaatttatg ggatgcgtat ccaaataatt
    18061 tttctggtgg agaaaaatta agattaaatt tagctcaagc tatggttaaa aagcctagac
    18121 ttttgttact tgatgaacca actgcatcac ttgataatca atctaaaata tatgtaaaag
    18181 aaaaaatgtt ggagcttaaa agtcaaggaa ctacaatgat aggaattttt catgatatag
    18241 agtttatgga aactgttata gataagacct ttactatgac aaaagggact ataagtgaaa
    18301 gtggggtagc ctagatgata aaactaattc ataatgcaaa tattgtatta gaagatatga
    18361 ttttagaaaa tggtcattta atcatagagg gagatacaat aaaaaaagta tcaaatgata
    18421 agattgatta cgcatttgac tttaatgaga tagatgaaat aatagatgca aaaaacttat
    18481 atgtaatacc tggtattata gatattcaca gtgatgccat tgaaaaggaa atagagccga
    18541 gaccaagtac attattacca tttaatatgg cattttatga actagataaa aaactacctg
    18601 caaatggaat aactacagtt tatcattcta tatctttagg ggatggagta ggagttagaa
    18661 gtattgataa ctctttaaag atgattaaaa atattgattc atataaaaat atagatagta
    18721 agagcataaa tcataaagtt catttaagat atgaagtgtt gtattatgaa ggtttagaaa
    18781 aggttttaga actattagat gaaaataaaa tagactactt atcaataatg gaccattctc
    18841 caggacaagg tcagtataca aatccaacat tttataaaga gtatgctact aaagtgtggg
    18901 gagttacaga aaattatgta gatacatggt tagatgattt agtaaatctg catgataatt
    18961 tagattggaa taaaatagct aatattatag gtatagccaa aactaaaaat ataaatgttg
    19021 catcacatga tgatgataca ttagaaaaaa tggatttcat aaaaaatata ggcataaatg
    19081 tatctgaatt tcctataact cttgaagttg caaaacattc aaagaaattg ggtgttctaa
    19141 cttgtttagg tgcaccgaat attgtaagag gtaaatctca taataacaat ttaaaagcaa
    19201 tggatgctat attggaagat tgttgtgaca ttgtgtgttc ggattatctt ccatcagcta
    19261 tgataaagtc tatgtgtata gttgcagaaa gaataaataa tttaaataaa gcagtcagtt
    19321 tatttacctc aaatcctgct aaagcagttg gtatatatga tgaaagaggt agtataaaag
    19381 aaaataaaaa agcagattta gttttagtag atattgatag tgaatatcca aaggttgtaa
    19441 atactattgt aaatggaaaa acagtttata aaagggaggt ataaagattg ctaacagagt
    19501 tacattgtca tacaagtata tcagataata attttactac tgaagaaata ataaaaaaag
    19561 cagcagaaaa gaatataaat atcttagcaa taacaaatca tgacacatta tctggattgg
    19621 atgaagctct tgtgcttgga gagcattatg gaataacaat aataccaggt atagaaattt
    19681 cagcctatga ttataaaaat agaaaaagag ttcatattct tggatataac atagatttaa
    19741 acagtcaaga aataaaaaat ttgtgcaatc caatggtatg tgacagacat aaagcatctg
    19801 ttaagatggt aaataaaatt atagatttag gatacaaaat atctctggaa gatgtaaaga
    19861 aatattcatc aaagactgga atatttaaac aacatataat gaaagctctt atagataaag
    19921 gatatacaga taatatatat tcaaatctat ataaagaatt gtttcataga ggaaacggca
    19981 aggtatatgt atctttaaaa tatgtggatt acagagatgc aataaaagct ataaaaaatt
    20041 ctggtggaat ttgtgtgtta gctcacccgg gtcaaatgga taattttagc gctattgaag
    20101 agatggtaaa agtaggatta gatggaatag aagtatatca tccatctcac aacaaagaac
    20161 tagaaaaaga gtcgctttct tatgctaaga agtataatct agtggtcaca ggaggttcag
    20221 attaccacgg attttattct caacataatt atgaacttgg aagcaagagc ttaaataaag
    20281 aagatttatt taagtttagg ttagctttag gaggggtatt ataaatgtct tttttacaag
    20341 taaaaaatgt aggtaaaagt tatggacaag ttaaggtgtt aaaagacata tctattgata
    20401 ttgaaaaagg agagtttatt tgtttactag gtccaagtgg ttgtggaaag agtacactac
    20461 ttagaataat tgcagggctt gaggataaac atggtggaaa aataataata aatgataagg
    20521 atatgactaa ttcaccacct gaaagtagaa attttggtat agtttttcaa tcatatgcat
    20581 tatttccaaa tatgaatgtt tacaaaaaca tagcctttgg actagaaaat aaaaacataa
    20641 gtaaatctaa tattgataaa aaagttaaag aagttctaga agttgtagaa ttaagtggat
    20701 atgagaaaaa atatccatca caattatcag gtggacaaca acaaagggtt gcattagctc
    20761 gtgcgattgc tttagagcca gactttttac ttcttgatga acctttatct gcattagatg
    20821 caaaagtaag acttaaactt agggagcaga ttagaagttt acatagaaaa ctaggcatta
    20881 caactataat ggtaacgcat gaccaagaag aagctctttg tttagctgat aagatggttg
    20941 ttatgaatag aggagaaata atacaagttg gaacgccaaa ggaggtatat aaaaatccag
    21001 aaacaccttt tgtagctgat tttataggaa caataaattt tatagatgat ggtattaata
    21061 agatagctat tagaccagaa gatataaaag tagagtctaa tagagattct aaagataagg
    21121 atataaaagt gggagaaatt ttagatatcg aatttagagg atttaactat agaattacag
    21181 ttgagtatcg ttctaagcaa atgaaattag atgtagtttc caaagttgca gaacaaatga
    21241 aactgtgcat aggttctaaa ataaacttta agatacctaa agaaggaatt gtacaatata
    21301 aatcagaggg atgtgcttaa tagctagggg tgaataaatg ataacatcaa aaaataaaag
    21361 aagaataagt gatagcacaa tacaaaaata tttcttgatt ttttcggtaa ttgtactaat
    21421 agtatttata ttatttccat tagtatcatt attaaaaaat gcatttatgg atacaagtgg
    21481 aaattttata ggaatttcaa actttagtaa atatgttgaa aatccaagtc ttatagcatc
    21541 atttaaaaat tcaatttttg tatctacagt ctcatcttta atatcactat gtttagcatt
    21601 tatatattca tacgcaataa caagaacaaa cataaagttt aaagattttt ttaaaactat
    21661 gggaatgttt ccactttttg caccaactat gttatatggt atttcactta tatacctttt
    21721 tggaaataag ggaatcttta caaccatggg tctggaagta ccactatatg gtccattagg
    21781 aataattatt tctcagattc tatttacatt tccacaagct tttttaatat tatctgtagc
    21841 actttcgatg gctgattata gactttatga agcagcagat agcttgggtg ctagtgaatt
    21901 taagaaattt tgcaaaataa caattcctgg tataaagtac tcattactta gttcattctt
    21961 tgtatccttt atacttgcat ttacagattt tggagcagct aaggtagtcg gaggaaacta
    22021 caatgtactg gctactgaca tatataaaca agtagttgga caatttaaca taccaatggg
    22081 agctactgtt tctatggtaa tgttaattcc tgtactaata gcatttacat tagataagat
    22141 ggcaagtaag aaacaaggta tgaccataac tgcaaagtct atgccatata agattaagga
    22201 aaataagttt agagatagag tatttttaat agtatgtagt tttataacat tgctaatttt
    22261 tattttaata ttcgtttcag ttataggttc agttataaag ttatggccat ataatttaag
    22321 ctttacaatg gaacactata catttaaagg tattttggga agtggaataa aaacatactt
    22381 aagcagtttg aaaatatcaa ctttaacagc aatatttgga actatatttg tattttttag
    22441 tgcttatatg atagaaaagg tggataagtt tcctaaatta agacaaacag cttacctttt
    22501 atcaatgata ccaatggcct tacctggctt agttttaggt atatcatata taactttttt
    22561 taactcagca agtaatccat taaatttttt atatggaagt acagcaatat tagttatagt
    22621 aaatgtagtt catttttatt cagtttgttt tataacctca aattcatctt taaaaatgtt
    22681 ggataaagag tatgaattag ttgcaaaatc cataaatatt ccattctata aagtattttt
    22741 taatataaca gttccaatgt ctataacatc aatacttgaa atagtagtat attattttgt
    22801 aaattcaatg gttactgttt cagcattaat atttttatat acacctcaaa ctcagactgc
    22861 atcaatatca atactaaagt tagatgagat aggatatata ggaacatcag ctgctatggc
    22921 agtattagtg cttttaacaa atataatagt aagacttttg tacgaatttg taacaaagaa
    22981 attaaaaaat aacacacaaa aatggcagca aaaagatgtt gcatagggga ggtaaaaatg
    23041 aaatttaaga agctagtttc tttaggagta ttatcaacac ttttattaag tgtaataaca
    23101 ggatgtagta ctgattcatc aggttcagat tcaaagtctg atagtaagac aaaaggggat
    23161 ttaacagttt acacagcaat tgaagaagat agtatagagc catatttagc tacatttaaa
    23221 gagaaatatc cagatattaa attaaatatt gttagggcat caacaggaga tataacagca
    23281 agattattag cagaaaagga taatcctcaa gcagatgttg tatggggagt tgcagccaca
    23341 agcttattgg ttgcagatga ccaggggatg ttagaaccat atgctccaaa aggttctgaa
    23401 gaaatagagt ctgcatttaa agatgataaa gaagttcctt cttgggttgg gatagatgct
    23461 tggatgacag gtataacagt aaacactaaa gaattaagtg ataagaacat cccagttcct
    23521 agttcttacg aagatttaat aaagccagaa tataaaggtc ttatatctat gcctaatcca
    23581 tcttcttctg gtacagggta cttaacagta tcagccctta tacaaattat gggtgaagaa
    23641 aaagcatggc agtatatgga taaattgcat gagaatattg gtgtatacac tcaatctggt
    23701 tcagctccag ctacatcagc agcttcagga gaatatccta ttggtatatc ttttggatat
    23761 agaggtataa agttaaaaga ggaaggctat ccagtagaag tagtatttcc aaaagaaggt
    23821 tctggatggg atatagaagc aaatgctctt gtgaaaaaag ataatataaa agaagcatca
    23881 aaagtatttt tagattgggc tataagtaaa gatgtaatga acgaatactc taagaactat
    23941 gcagttacta ctatttctac aggaaatcca ataccagaag gattccctaa aaagccatta
    24001 gagcaaatga tagataatga tttaaaatca gcagcaaaaa atagagaaga tatactaaat
    24061 aaatggatat caaaatatga tggtaagaca gaaaaagaga gttaagtaaa actatacaaa
    24121 tggagtaaaa agaaagaatt tttttaagta gattttatta taataatata agttgagaat
    24181 aatataggtc aagaaaagtg aatattctat aaaaatgtat agagtaagta taccattcag
    24241 tttgaatggt atatttttta tttttagaga ttaatttaga aatagatata ctacagttta
    24301 aaatagtata ttttaatttc attattaaag tatagatatt atatataaag taaataaatt
    24361 attgagcgat tattaataag atatgtataa attttaattt gggaacttgt gttatatatc
    24421 atgaaaaaag agaaaaaata aaaggtaaat tgtttcttga taacttgaaa ttggaagtga
    24481 gaatatttaa ctagagaaga attatttaag tttaagtcag ctttaggagg aggaattata
    24541 aatgtctttt ttacaagtaa aaaatgtagg taaaagttat ggacaagtta aggtgctaaa
    24601 agacatatct attgatattg aaaaagggga gtttatttgc ttattaggtc caagtggttg
    24661 tggtaaaagt acattgctta gaataattgc aggacttgag gataaacatg atggaaaaat
    24721 aataataaat gataaggata tgactaactc accacctgaa agtagaaact ttggtattgt
    24781 tttccaatca tatgcactat ttccaaatat gaatgtatat aaaaatatag cctttggatt
    24841 agaaaataaa aatataagta aatctgatat agataagaag gttagagata ttttagaagt
    24901 tgtagaatta agtggatatg ataaaaaata tccatcacaa ttatcaggtg gacaacaaca
    24961 aagggttgca ctagctcgtg cgattgcttt agagccagac tttttgcttc ttgatgaacc
    25021 tttatctgca ttagatgcaa aagtaagact taaacttagg gaacagatta aaagtttaca
    25081 tagaaaacta ggcatcacaa ctataatggt aacacatgac caagaggaag ctctttgttt
    25141 agctgataaa atggttgtta tgaataatgg agaggtaata cagattggaa ctcctaaaga
    25201 agtctataaa agtccaaaaa caccttttgt agctgatttt ataggaacaa taaattttat
    25261 agatgatgat gtgaatatga tagcaattag accagaagat ataaaggtag aatttaatag
    25321 agactttaaa gaaattgatg taaaagtagg agagattttg gatatagaat ttagaggatt
    25381 caactataga attacagttg aatattgttc taagcaaata aagttggacg tagtttcaaa
    25441 ggtagtggaa caaatgaaac tatgtgtagg ttctaaaata aacttcaaga tacctaaaga
    25501 aagaaccata caatataaat cagagggatg tgcttaatag ctagaggtgg ataaatgata
    25561 acaataaaaa ataaaagaag aataagtgat agtaagatac aaaaatgttt tttagttttt
    25621 ttagtgattg cattagtagt atttatatta tttccattaa tatcactatt aaaaaatgca
    25681 tttatggata caagtggaag ttttgtagga atttcaaact ttagtaaata tattgaaaat
    25741 cccactctta tagcatcatt taaaaactca atttttgtat ctacaatttc atccttaata
    25801 tcattatgtt tagcgtttat atattcatat gcaataacaa gaacgaatat aaggtttaaa
    25861 aactttttta aaactatggg catatttccg cttttcgcac cagctatgct atatggtatt
    25921 tcacttatat acctttttgg aaacaaggga atctttacaa ttataggcat agaagtacca
    25981 ctatatggtc cattaggaat aattatttgt caaatactgt ttacatttcc acaggccttt
    26041 ttaatattat ctgtagcact ttcgatggct gattatagac tttatgaagc agcagatagc
    26101 ttaggggcta gtgaatttaa gaagttcttc aaaataacaa ttcctggtat aaagtattca
    26161 ttagttagtt cgttctttgt atcttttata cttgcattta cagattttgg agcagcaaag
    26221 atagttggag gaaactacaa tgtattggct actgacatat ataaacaagt ggtaggtcaa
    26281 tttaacatac caatgggagc tactgtctct atggtaatgt taattcctgt actaatagca
    26341 tttacagtag ataagatagc aagtaaaaag caagggatga ctataactgc aaagtctatg
    26401 ccatataaga ttaaggaaaa taagtttaga gatagagtat ttttaatagc atgtagtttt
    26461 ataactttat taatctttat tttgatattt gtttcagtta taggctcagt tataaaatta
    26521 tggccatatg atttaagctt tacaatggaa cactatacgt ttaaaggtat atcaggaaat
    26581 ggaatcaaaa catatttaag cagtttaaaa atatcaattt taacagcaat atttggaact
    26641 atatttgtat tttttagtgc ttatatgata gaaaaagtgg ataagtttcc tagactgaga
    26701 caaatatctt attttttatc aatgatacca atggcattac ctggtttggc tttaggtata
    26761 tcgtatataa cttttttcaa ttcagcaagt aatccattaa attttttata tggaagtaca
    26821 acaataatgg ttatagccaa tatagttcat ttttattcag tttgctttat aacttcaaat
    26881 tcatctttaa aaatgttgga tagagagtat gaattagttg caaaatctat aaatattcca
    26941 ttttataagg tgttttttta tgtaacactt ccaatgtcca taacatcaat acttgaagtg
    27001 gctgtatatt acttcgtaaa ttcaatggtt actgtttcag cattaatatt tttatatata
    27061 cctcaaactc aaactgcatc aatatcgata ctaaagttag aagagatagg gtatataggg
    27121 ccatcagctg ctatggcagt attagtgttt ttaacaaata taatagtaaa acttttgtat
    27181 gaattgacaa caaagaaatt aaaaactaag acacaaaaat ggcaacaaaa agaagttgca
    27241 tagaggaggt aaaaatgaaa tttaggaaat tagtctcttt aggaatatta tcaacacttt
    27301 tattgagtgt aataactgga tgtaataatg cttcatcagg ttcaaattta aagtctaata
    27361 ataagataaa aggagagtta acagtttata cagcaattga agaagatagt atagatacat
    27421 atttaactac atttaaagag aaatatccag atattaaatt gaatgtactt agattatcag
    27481 taggagatat aacagcaaga ttattagcag aaaaagataa tcctaaagca gatgtgttat
    27541 ggggggttgc agctacaagt ttgataattt tagatgacaa agaaatgcta gagccatacg
    27601 ctccgaaagg ctttgataag gtaaatcctg tatttaaaga tgataagaaa gttccttctt
    27661 gggttggaat agatgcttgg atgactggta taacagtaaa tactaaagaa ttaagtgata
    27721 agaacatacc agttcctagt tcttatgaag atttaataaa gccagaatat aaaggtctta
    27781 tatctatgcc taatccagct tcttctggaa caggatattt aacagtatca gaacttatac
    27841 aaattatggg tgaagaaaaa gcatggcagt atatggataa attgcataaa aatattggtg
    27901 tatacactca gtctggttca gcgccagcta tattagcagc ttcaggagaa tatcccattg
    27961 gtatatcttt tggaaataga ggtataaagt taaaaggtga aggttatcca gtagaagtag
    28021 tatttccaaa agaaggttct ggatgggaga tagaatcaaa tgcacttgtg aaaaaagata
    28081 atataaaaga agcatcaaaa gtatttttag attgggctat aagtaaagat gcaatgaatg
    28141 agtattctaa gaattttgca gttactacaa ttgacacggg aaatccggtg ccagaaggat
    28201 ttccaaagaa tccattagaa cagatgatag ataatgattt aaaatcagca gcaagaaata
    28261 gagaggctat actaaataaa tggatagaaa aatatgatgg aaagacagaa aaagagagct
    28321 aagccttgca ataaaattta gtacaaaaag cgcctagaat ataagtttta tatctaggct
    28381 cttttgatat atttatttaa aatgcatatt tctatataaa aataaatagt ttagtacctt
    28441 gattaaatta aataaaaacc ttaaaataaa gagatgtaaa gaaaaactaa aaacgaataa
    28501 aaggtgaatt tatatgataa aagaaataat agtagtagag ggtagagatg acgttacagc
    28561 agttaagaga gcaatagatg cagaacttat aactacaggt ggatttggat ttccaaaagg
    28621 tgtaatggag cgtataaaag ctgctaatga aagacgtggt gttataatat ttacagaccc
    28681 agattttgca ggtgaaaaaa taagaaaaaa aatagcatca gaagttccag gatgtaaaca
    28741 tgcatttcta ccgagagagg aagctaaaaa ggatggagat ataggtattg agaatgctac
    28801 tcctcaaagt ataatagcag ctctgaataa agttagaact gaaaatacag aaaaaagaga
    28861 tgaatttaag caagttgact taattagaaa tggtcttata ggtaatgaag aagcatcact
    28921 tagaagagat gcacttggaa aaatacttgg aattggatat ggaaatgcaa aacaattttt
    28981 aaatagattg aataattatg gagttgaaag agaagaattt atagatgcat taaaacaaat
    29041 ctaaacgtaa tgtttaatgt atgaaaaata tgtatataaa ataaattgtg gaattacggt
    29101 taatttaaga tgaattattt ataatataca ccttatttta aatcaatgta aatacatata
    29161 aataagtata attaataaaa ttaaatatca aaataagtta aaaaggagta aataatggat
    29221 agactatctt cacataatgc aacaaaggaa gtagtacaaa aatataattt taaattttca
    29281 aagtctttag gtcaaaattt cttgatagat agtaatataa ttgataaaat cttatctgga
    29341 gctagaatca caagaggaga taatatcata gaagtaggac ctggtatagg aactcttact
    29401 cgtgagatgg gtaaaatagc agaaaaagta gtggctatag aaatcgatag aaatttaatt
    29461 cctatattga aagatacctt gtcagattta gataatacag aggttgtaaa tcaagatata
    29521 ctaaaagtag acatacagga attagtaaaa gataaattaa atggtgggcc agttaagctt
    29581 gtagcaaatc ttccatatta tataactact ccaatagtta tgaaattttt agaggaagac
    29641 atacctgtaa cagacatagt agtcatggtt caaaaagaag ttgcagatag aatgaatgct
    29701 attccgggta ctaaagatta tggggcatta tctatagctg ttcaatacta ttgtgataca
    29761 gagattgtgg caaaagctcc aagacatatg tttataccac aaccaaatgt agattccaca
    29821 gttataggtc tacatgtaag agataaaaga aaatatgatg ttcataatga agacatattt
    29881 tttaaaacag taaaagcatc ctttggacaa agaagaaaaa ctcttcttaa ttcgttggga
    29941 ggtcttggtt ttcttaataa agatgaaata agagaagtat taaaagaagc taatatagac
    30001 gaaaaaagaa ggggtgaaac tcttagtata gaagagtttt cagttctttc aaatataata
    30061 aatacaaaag tatcttctaa atagaaggta ctttttttaa ttttgttaac atatattgtt
    30121 tagagaaact gtgttaaggg gggattgcag tgggttctaa aagaaagttt aaaagagact
    30181 atataatact agaagctaaa gacataaact ttagatacaa agaacgagta ctgccaaaag
    30241 cttttgcaaa agttgaagta aatgatgaaa aagcaattat tgcattatat gtagaaaatc
    30301 taaaatatgt taaagatggg tataaagcag tagcaataaa agatgaccat ggattaatag
    30361 acttaggaaa tataatttta aatgaacaag gaagaggtga atttgtttta gatttagaag
    30421 attatgatga tgagataaaa ggtattgcat tattgtatga aagaaatata cctttaattg
    30481 gatttaaagg aaataagctt gaaaattatg aggacatatt atttcaagag gtagaggaag
    30541 aagaatatga agaatatgaa gagttggaag aagaagacga agagatagag gataatgagg
    30601 aaagtgatag agacgaaggt agtgaagaag aatatattat agaagaatat gatgaagatg
    30661 agtatgaaga ctattgggaa gatgatgaag atattgacga aggagagtta aaagcaagaa
    30721 cggaagtaga agattctggt gatgaggaaa tagaagagtt caataattat aaggaaatag
    30781 aaaattataa tagggttaaa gtacaggaag aatatataga catagaatat gatgaggaag
    30841 aagaggaaga agaagaagtt tttgaagtaa atagagaaga agataggttt gtttcctcaa
    30901 aacctactaa atcaaagaaa gttcaaaaag atcagaataa tattgaaaaa ataaaaagag
    30961 ctaatggagt aagtcaagga gagagtggaa ataaacaaac cagtacatta cttatgccaa
    31021 gacaaataaa aaaaggtttg aaattattta aagaagtaaa accttttgta acagattata
    31081 tagataagac aagatggtgg aaaatagaga taaatcctac aacattgtgt ggatatacaa
    31141 tgccttattt aggatattta aacgtgttga attacaccat gtatagtgat gcagttttac
    31201 attcatataa atacagacac tatttatttg gagtccagta tgatgaatat aataagagaa
    31261 aacattatat ttatgctatc ccaggaaata gaaatgaaca gccagacaat ggaagcacag
    31321 gatttttaag atatcaagct tgtgataata gaacaaatag tttaggatat tggctatgtt
    31381 ttatagattc tagacaaaga aatatagtta ggtaaaaata ataaaaatga aaaaatttag
    31441 actcaattct tattttactg aaaaaattaa tgaaatgtag tacaaaactt taaaaactat
    31501 tgataatatt taaatattac actacaatat atcttgaaat acctattata ttatggagga
    31561 aaattatgat aaacgagcaa agaattttaa atgagtttct tgaattagta cagatagata
    31621 gtttatcttt aaaagagggg aatgttgcta aagtattagt taaaaaatta gaagaaatag
    31681 gttgttcagt agtaatagac aatgctggtg aaaaagcaaa tggagaaact ggtaatataa
    31741 tagctacatt aaaaggaaat aaagaaggaa agaaaatatt atttagttca catatggata
    31801 cagtaactcc aggtataggg gtaaaaccta tagtggatga agcaaatgga ataataaaaa
    31861 gtgatggtac tacaatatta ggttctgatg ataaagcagg tatagcagct atattagaag
    31921 gcttaagata tataaatgaa aataatatag aacatactga tatacaagtt gtattctcta
    31981 tatgtgagga atgtggatta gttggagcta aaaatttaga ctatggaaaa atagattctg
    32041 aatatgcatt tatattagat agtggtggtt ctccaggtga aataatagta aaagctcctg
    32101 cacaagatgt aataaatgtt aagatattag gaaagactgc tcatgcaggt cttgaacctg
    32161 aagctggaat aagtgctata atggtagctg ccagagcaat agaaaatatg aatttactta
    32221 gaatagacga agaaacaact gctaacatag gaattataaa cggtggaaca gctacaaata
    32281 tagtaacagg agaagttaat ataattgcag aagccagaag tctaaaagaa aataaattag
    32341 atgttcaaac taaacacatg gtagagactt ttgaaaaagc tgcaaaagat tttggagctc
    32401 aaattgagat agatgtcaat agagcatata caccaataga tgttagtgag gattctgaaa
    32461 taataaaact tgcaaagaag gctttttcaa acttaggtat agaaggtcat actgaatcta
    32521 caggaggagg tagtgataca aatatattaa gtaagaatgg tattgaagct ataactttag
    32581 gaattggtat gaaaaatgct catacactaa gtgaacatat agctataaaa gacttatatg
    32641 attctgctaa aatggtagtg gagataataa aggaagcata aatttaaata atagtgtata
    32701 atatatatat cgcctagctt aagctaggtg atttttcatt ttatagaaga ttgataatct
    32761 ctattgaaag taaagagtat acatttgcat ataagagagt gtttaattta tgttcaaatg
    32821 taggttctta ataatattta gattgtttca taagggagga ataaatatgg aaacaaggta
    32881 tgaagaagca aataaaataa caatacaatc aatactttgg aatgtagtat taacaataat
    32941 taaggttata gctggtgtta ttggtaattc tagcgctatg attgcggatg gattacattc
    33001 agcatcagat attataagtt ctataggagt actaataggc aattatgtat cttctagacc
    33061 tggagataga gagcataatt atggtcatga gaaagcagag acattagtat catttgtgtt
    33121 atcaatattg cttattttcg tttctataac aataggtata gaggcaatta aatctttatt
    33181 taacttagat gcattaagtg ttccatcaat attaccatta gtagtatcag ttatttcaat
    33241 cctgataaaa gagtatcaat atagaataac aataaaagtt gctaaaaaga taaattcacc
    33301 agccctaaaa gctgatgctt ggcatcatag gtcagatgct ttatcttcag tagctgcttt
    33361 tataggtata ggtggttcta ttttagggtt taaaccatta gacccaattg cgtcagttgt
    33421 agtggctata tttgtagcta aagtaggaat tagtatactt ataagttctg ttaatgaatt
    33481 gatggatgtt tcagttgatg aagaagagat aaaagaatta aaatttattg tagcagatac
    33541 agaaggtgtt aaaaatcttg gtgatataaa gactagaaaa catggagcta tggcatatgt
    33601 agatttgact atatgtgtgg atgaaaattt gacagttaag caaggtcatg atattgctac
    33661 caagcttgaa aaacatataa taaaacatat ggaatttgta aaaggcataa cagttcacgt
    33721 tgaaccatgt actaattgcc aaggaaataa gtgtaataat taattaaaac ttaaaaaaaa
    33781 gtgtaaaaag ttatataatt gttttatgat tataaactct aggggtggtt aaatgagcaa
    33841 gaaaagaaaa ctaaagaaaa aagctgcaat attaatagga gtagttgttt tctttataat
    33901 atactttata tcctttagaa taggaataaa tatggaaaag gataaaagtg aagtgccaaa
    33961 gaaggtagaa aaagaagaag aagataatag gtctttaagg actcaaataa aagagaagaa
    34021 gaaggtttat atatcagata aaaatataga aaacataaaa attgaagaag atatgtggga
    34081 tgaaataaag ttatttttct cagattttaa gaaagtaaga aatactactt cttcttacga
    34141 agcagtttat gaaggttatt ctgatgatgg agttaaattt tctactgacc ttaacttttt
    34201 tagagtatat actgtaaata aagaagaata ttataaagta ccagttagtt ctaaggatgc
    34261 ttttaagaaa cttttagatg agagcatata tacttcgttt gaccttttaa gtcattataa
    34321 agattggaaa gaggttacta taactcatgg aaaagaaatt aaaaaagttc atagatggaa
    34381 atttgatgat ttagcacata agatgatttc aaagagggtt gtaggaaaag ttcaacctga
    34441 gaaaagtaag gaaagaagtg actataattt tactataaat ataaaaggtg ataattttga
    34501 tgtaaaagtt gatacaatgg gaaaagatta tgtaaaagtt acttctaaga aaaatgtatc
    34561 ttactatgag gttcatacaa gtttatttga ttaccttaaa aatgagatat ttgaaattgg
    34621 aaagaaataa gactttaaga aggctatcaa attagatatt tgatagccta aattatttta
    34681 tatattattt ttcaaccaac aagtctgcaa ccttataaat tggaccagca ccagcagtta
    34741 ttactaaatc attatcctta acattttcac gtaagtattt tactatatct tcaaattctt
    34801 ttatatagat ggcatctaca ttgttttgat ataatttctc aaccaagtcc ttagaatgta
    34861 tatctcctgg gtctttttcc ctagcagcat atatatctgt tatgataact ttatcagcag
    34921 catagaatgc ctctgaaaat tcacctaaaa gagattttgt tctagtataa gtatgtggct
    34981 ggaagataca ccataaagtt gattttttta attttttggc agcagctaaa gttgctttta
    35041 actcagttgg atgatgtgca taatcatcta ctactaaagc attcttataa taccctttag
    35101 tttcaaatct tcttccgaca cctttataaa tttttatatt ttttcttata gtctctaagt
    35161 caataccaga aacaagagct gccattatgg cagaagaagc attgtatatg ttatgaagac
    35221 catatacaga aagttcaaat tctcctaaat ctcttccttc atattctatt ctaaatattc
    35281 catgaccact attgtcaaag tgaatatctt ttattacagc atcattgtca gaatctctgc
    35341 cgtattttat aatagtagct ttaacatcat acaatatgtc atcaatattt tcatcatcgc
    35401 catttattat gaaatagcct tctgggggta ataattttcc aaatttgtta aaagaagctt
    35461 tgatttcatc tattccagag aaataatcaa gatggtcttc ttcaacattt aaaactatag
    35521 atatctttgg gttaaaatta aggaaactat ctacatattc acatgcttca gttataaagt
    35581 ggttggaatt acctatctta acattaccac ctatcatact taagttacca ccaactagta
    35641 tagttgggtc taagtctgca tattcaaaga ttgtagataa catagatgtt gtagaagttt
    35701 ttccatgtgt tccagaaact gctatagaat ttttatattc cctcatgatt tggcctaaaa
    35761 aagtcgctct gttcattacc aatttatttt tttctttagc agccacaagt tcttcattgt
    35821 ctggatgaac tgctgctgta taaacaacca tatttacatc atctgatata ttatcttttt
    35881 tctgaccaat ataaatagtt gccccttggt ccctaagttt gtctaatagg tatgattcgt
    35941 ttgaatccga acctgaaact tgataacctt tattgataca tatttcagct aatgcactca
    36001 tgctgatacc gccaattcct ataaagtgta tattcataat aaaaccacct ttctttttaa
    36061 ttaagtccta caatatgaac tatttctgtg gtataataat ataatgttca atattttata
    36121 attaatatgc atataatatt atatgtattt tatttttgaa tataataaaa cataagtaat
    36181 attataccac aataagaatt gttatgcatt agttagttta acgttatata aaggtacttg
    36241 taggaggaaa taataatgaa atttaaaaga acagagagaa ttggagcaat agttaaaata
    36301 ctgtctgata atccaaataa aatatatact ttaagttatt ttacaaatca atttaatgca
    36361 gcaaaatcaa caataagtga agatttatta gttgtaaaaa atgtatttga gaagctacat
    36421 ttaggtaaag ttataactat ttcaggagca gcaggaggag taaaatatat acctaaaact
    36481 tctatagcag aaaatgcaga gttcttaatg gaattatgcg agagaatatg tgacaagtct
    36541 agaatactat ctggtggttt tttatacttg attgacctaa tttacgaccc tacaatagct
    36601 gctaagatag gaaagatatt cgcatcaaat attgagtatg tagatgcaga ttatgttgtg
    36661 acaatggaaa caaaaggtat acctatggca cttatgactg caaaagctat gaatttacct
    36721 ctagttataa taagaaaaga tataaaggta tcagaaggtc ctacacttag catgacatat
    36781 gtgagtggaa acagttctaa agttgaaagt atgagtttgc caagaaaagc tcttaaacca
    36841 gatagtaaag ttataattat agatgatttc atgaggggtg gaggtactat aaaaggaatg
    36901 gtagacctta tgaatgaatt tggtgcagag gttataggta ctggagtatt catatctaca
    36961 acgaatcctt cagaaaaaat ggtaaaagat tatatttcgt tgattcaatt ggatgttaat
    37021 ggagataaaa tagtagtaga accaaactta aagactttta aagatgaata tagaaatgaa
    37081 gatttagacg aagaagaaga ttcagaattt gaatttgaga tagatgaaga ttaataaaaa
    37141 aaatatttag aaaaatatgt aaaaataaaa agaggatatc cctagttgtt catagaatta
    37201 tttaaaaaac agtttatatt aatttattcc agcgataata attttaatat aaattaattt
    37261 ctgtcaaaat caaaggggga tattttggta tgaagataac tgacgtaaga gtaagaaaat
    37321 taacagaaga aggtaagatg aaatgtatag tttcaataac tttcgataat ttgtttgtag
    37381 tgcatgatat aaaagttata gaaggccata atgggctatt tatagcaatg cctagcagaa
    37441 aagtaggcga aggaaatttc agagatatag ctcatccaat aaatgcagaa atgagacaag
    37501 ttttagaaga tgcagtttta caagcgtatc atgaagcatt agttcaatgg gaagtagctg
    37561 ctgaataata ataaatatat aaaatggaga gagcccgaac atttaggctc tttttaaatg
    37621 tagaatttct attaaaatat gtacttttta gtaaaaaagt gctatattat tagtgataat
    37681 gtatatgttg tatacaaatt gttagataaa tgtattataa tgtatattga ttaagagaat
    37741 aatacgaata aaattaaatt agattgaatg gagtgtatca atgaacttta aagctataat
    37801 ccttgctgct ggcaaaggaa caagaatgaa gtctaaatac ccaaaagtta tacataaagt
    37861 atgtggaaaa gaaatggtta accatattat agatgtttca aaaaaatcag gggtaaaaga
    37921 tactgtcgtg attttaggac atgaagctga tgtagttaaa gaaaagcttg cagaagaaat
    37981 tataatagcc atgcaaacag aacaacttgg gacaggacat gctgtaaaga tggctaaaga
    38041 atatattaat gatgaagata caatagttgt actttgtgga gatactccac ttataaaaga
    38101 agagacacta aagagactat ttgaatatca tatagaaaat aaatatcatg ctacagttct
    38161 tacaacaaga gttggaaatc caacaggtta tggtagaata ataagagata agaagggtga
    38221 ccttcttaaa atagtggaac aaaaagatgc aaattcagaa gaaaagatga taagtgagat
    38281 aaactctgga atttattgtt ttaatggaaa gagtcttaga gaagcactag atttgctaaa
    38341 taataacaat tcacaaggtg aatattattt aactgatact gctaaaataa tgagagataa
    38401 aggattaaaa gttggtgcat ttgcaggttc aactattgaa gaacttatgg gcgttaactc
    38461 aagagtagaa ttatcaaagg cagaagaaat tatgagacga agaataaatg aatctcatat
    38521 ggtaaatgga gtaactataa tagacactaa ctctacctat atagaatctg atgttatgat
    38581 aggcaatgat actataatat atccaggtgt tatgttacaa ggaaaaacaa ggataggttc
    38641 agattgtata attggaatga attcatctat aacaaattca gaaataggtg atggtacaga
    38701 aataaaaaat tcaactataa ttgatagtaa ggttggagaa aattctactg ttgggccata
    38761 tgcatactta agaccaaaga gtgacttagg aaataatgta aaaataggcg attttgtaga
    38821 agtaaaaaat gctattatag aagatggctc aaaagcatct cacctttctt atataggaga
    38881 tgcacatgtc ggtaaaaatg taaatattgg atgtggagta gtatttgtaa actatgatgg
    38941 taaaaataaa tttaaatcaa tagtaaaaga caatgcgttt ataggttcaa attctaattt
    39001 agttgcacca gtagttgtag aagaaaaagg atatatagca acaggttcaa caataacaca
    39061 tgatgtccca gatggtgctt tggcaatagc aagagaaaga caagttatca aagaaggatg
    39121 ggtagaaaaa aagaatcaaa aagacgacca aagtaagtaa ttatgtagtt tattattttg
    39181 gctaaaaaat ttagctcgaa attattataa attatattca ttagtatttt aaaattttca
    39241 ggagggttac ataaatgaat actagcggaa gcgagattaa aattattgca ggtaattcat
    39301 cgaaagagtt agcacaaaaa atagctgact atataggtgt atcagtacta gattgtgaag
    39361 taggtacatt tagtgatggt gaaatatgtg taaacatgaa tgagacagta agaggttgtg
    39421 atgtgtttgt agtgcaatct actaacagcc cagtaaatga taacttaatg gagttattaa
    39481 tcttaattga tgcgttaaag agagcatcag caggtagaat aacagcagtt attccttact
    39541 atggatatgc aagacaagac agaaaggcta aggcaagaga tccaatcact gctaaattag
    39601 ttgcaaattt aataactgct gctggagcag atagagtatt aacaatggat ttacatgcag
    39661 ctcaaataca aggatatttt gatataccat tagaccactt actaggtgga acaatattag
    39721 caaattattt taatgaaaag aaaatagaag atttagtagt agtatctcct gacttaggaa
    39781 gtgttactag atcaagaaaa tttgctaata ctttaaatgg agaagttcca atagctataa
    39841 tagataaaag aagaccgaaa gctaatgtat gcgaagttat gaacttaata ggtgatgtta
    39901 aaggtaaaaa tgttatacta ttagacgata tgatagatac agcaggaaca atagttaatg
    39961 ctgcaaatgc acttaaagag tttggagcaa aagatgttta tgcttgttgt actcatggtg
    40021 tgttatcagg tccagctatt gaaagaattg ctaattcaga aataagtgag ttaatagttc
    40081 ttgatactat acaacttcct gaagagaaaa gaatagataa gataaagata aaaacagtag
    40141 ctcctttatt tggagatgct ataagaatga tattctctaa tgaatcagta agtaaattat
    40201 tctaatttat tgtatttatg agagttgttt aaaaggatat aaaccttttg agcaactctt
    40261 ttttgatttt ataattatgg aatgttattt ttttcttgtt agaaaggtat atattattgt
    40321 aaaaagagat tatagtttaa aattatgtat atttaagtaa aataaactta ataaagggca
    40381 tattattcaa aatattatga atataaaaaa tattacaaaa aagtattgaa aaaattcaga
    40441 ttatgtagta ttatgtaaat ataaataaag aaagaggtgt ttccaatggg aaggttagaa
    40501 gatgaacccc taataattaa atgattcagt cacaatctaa agtgaatata aaaatctatt
    40561 gaaagtttat ttataagaat tgaaaatagt atctattcta tctttagtgt ttgtgagagg
    40621 gttctggatt atataagatt actgtaaaga agtaattttg cacaataagc atatctgtaa
    40681 atgaagagtt tcgtttatat aatgattgtg gctgaatcat aactataata gatagaatgt
    40741 ttataataga tgattttata aaatactata ttgtaatagc ttttagcttt caaataaata
    40801 ttagcaaagg atggaaatga caatgataaa actaaatgat aatttataaa tatctcctta
    40861 cgatatataa taattgtagg gagatgtttc ttttttttgt agagtataat ttttctagaa
    40921 attctgtttc aatatgtaaa aagttggtat atataaatag tataaacagt gttgctaaaa
    40981 tatggtagat attatataat ataaatggat atgtatatac attatgagtt atataatttg
    41041 aatttaataa aatagaaacg ttgatttatg ttctgtaatg tgggcacctt ggagcatatt
    41101 gagttagtgg tgcaaccggc tatgaaattg tatttattta taaatactat tattttcata
    41161 gcttttttta tttaaaaaat ggttatatgt tagaaataaa tttggttagt acgtttaaat
    41221 aattttactt agatttttaa ccagatatat tataagtaag tattaattaa aaaatttaag
    41281 ggggaataaa aatgaagtta aaaaagaata aaaaaggttt cactttagtg gaattattgg
    41341 tagtaattgc aattataggt atattagcag tagtggcagt tccagcttta tttagtaata
    41401 taaacaaggc taaggtagca agtgttgagt ctgattatag ctcagttaag agtgcagcat
    41461 tatcttatta ttcagatact aataagatac cagttacaac aactgataaa gctggtttaa
    41521 ctattttaga gacttatatg gagtctttgc ctgataaagc tgatataggt ggaaaatata
    41581 aattgattaa agttggtaat aaattggtat tacagatagg tacagatact gaaggagtta
    41641 cattaacaga agcacaatca gcaaaattat tgagtgatat aggtaaagat aaaatatata
    41701 caggtgttac aggagataat tttggagacg aattaacaga tactacaaaa atagataata
    41761 aagctctata tatagtactt atagataata ctgtgatgga ctcaacaaaa taattaaaac
    41821 tatattttta taaagaatat tgtaaattta atgaaaaata taatataaaa gacaggaaga
    41881 aaatcttcct gtttttttat actattttct ttaatgttca attatgaatg atgatataat
    41941 tgagatagta aaggagtgaa acctgtggct aaaaaagtta gaataggaga caaactggtc
    42001 gaaaaaggat atataacaga agaacagctt aaatgggcac tatcagaaca aaaaaacagt
    42061 ggaaaaagac taggagaatt tctagtacaa gaagggctta tagatagtaa cttactaata
    42121 agtgtattaa aagaactctt ggatatagaa agtatatttt tagaagggac agaaatagac
    42181 actttagcta caaaaatggt tccagaaaat atatgcaaaa gatatactgt atttccattt
    42241 aaaattgatg gaaataaaat atgtctagcc atgtcagacc cacaagatag agaagcagtt
    42301 caagatgtaa ggcgtatgtc tggtaaagac gtagagatat ttatatcaag tacagaagat
    42361 ataaacaaag caataggtca tgcttatgca cattcagaaa taaataaggc aatgactgag
    42421 tacaacaaaa atagaactgg tggagtaaga gaaacagtaa tacttgaaga agatgtaaat
    42481 gctgctccaa tagtacgatt agtaaataac atactagaaa atgctgttag aatggaagca
    42541 agtgatatac atatagaaca aagtgaaaat tacatgcgtg ttaggtttag gatagatgga
    42601 atgttaagag aatatatgag gatgaactca gcaccatata aggcagtaat cagccgtatc
    42661 aagataatgt cagatataaa tatatctgaa aagagaatac cacaggatgg tagaatctat
    42721 ttaaaagtgg ataataaacc catagatttt agggtttcta caatgccaac taacagggat
    42781 gaaaagatag ctatgagggt tcttgataag agtaatttta tggttagtaa agaagtacta
    42841 ggaatagacg aacatggctc taagatatat gatgaattga taaacactcc ttatggtctt
    42901 atattggttg ttggccctac tggaagtggg aaaaccacta cattgtattc aatgttaaat
    42961 caactaaata cagaaaatag aaatctatta acaatagaag accctataga gtatgaatta
    43021 cctggtgtaa atcaatctca aataaatgaa aaggcagggc ttactttcgc cagcggactt
    43081 agagctttta tgcgtcaaga cccagatata ataatggttg gagagataag agatactgag
    43141 acagcagaaa tagcaattag agcttctctt acaggacatt tagttttaag tacattacat
    43201 gcaaatacag cagttggagc aatttcacgt cttttagata tggatgtgga aagtttttta
    43261 ataacttcat ctgttttagg tgtaatatct caaagactta cacgaaaaat atgtgagcat
    43321 tgtaaggttt catatgaagc agatattgga gaaaaaaagg ctcttggtat tgatgtaaat
    43381 gaatcagtaa ctatttatag aggaaaaggt tgtgaaaggt gtaataatac tggatataaa
    43441 ggacgtctag gtatatttga aatgcttgaa attactccag aaataaaaga gttaatagat
    43501 tcatctgcaa atcaaagaga aattttaaag atggctagaa aacaaggtat ggttagctta
    43561 aaagaggata tagttaaaaa agttttaaat ggaaagacaa cagtagaaga aatgattaga
    43621 ataattttga tgactgatta aaggatgata ttatgaaaag ttttaaatac acagtaataa
    43681 aagaggacgg aaaaaagcaa actgatgtga ttgaagccaa cgattttaat gaagctagaa
    43741 atcttcttag aaagagaaat cttagagtta ttgaaatcaa agaaagcaaa aataaaaata
    43801 taagattatc tagtagaaag agaaaaaaag acttaggagc tgaccagata agccactttt
    43861 gtagacaatt tgctattata gtatcttctg gtataaatag tatttctgga ttagaaacat
    43921 tagctagaag gtcaactaac attacattaa gagaagaaat taatcgtata gtagcagaaa
    43981 taaaagtagg ttctactatt gcagattcaa tgctttcatc aaaatcaaag tttccaaagt
    44041 tacttggtgc tatggtagct acaggtgaag ctacaggtaa attagatgaa gtattaaaaa
    44101 gtatggcttc attttatgca agtgaacaca gggttaaaca aaagcttaga aatgctgcaa
    44161 catatccatt aatagttttg atttcttcat ttataatgat ttttatattt acaacattta
    44221 tggtgcctaa gatgattaat tcaattacaa cagtaggagc aactcttcca cttataacaa
    44281 gaattgttat ggggtttggt atgtttatga agaagttttg gataatagta cttttagtta
    44341 ttatattatt tatctatcag tttaaaaaat atctaaaaac gtcagttgga agggctcata
    44401 aagatagagt tataaataag atacctgttt taggaaaagg aataaattgt atggtagcta
    44461 cacgattttc aagagcattg tatctatttg tatcaacagg atacccatta gttcaagggc
    44521 ttgattatat tatagatagt gtaaataaca ctatggcaga gaagatttta gcatcagcta
    44581 aagatggaat aacaagagga gaaggcttag cagaaaattt agagagatat acgtattttg
    44641 actctgtgtt ggtacagatg atagctatag gggagcagac aggggaatta gagaatataa
    44701 gtagacagat ggcagaattt tatgagtatg aatccgagat ttatttaaat aggatggcct
    44761 caatgataga gcctgtttta ataattgcag ttggtattat agttgcgata ctggttgtat
    44821 caatattcat gccaatgctt agtatatatg atgccatgta ggagggaaag gacaaatgaa
    44881 caaggtttat gtatcatatt atggcgattc tatttccata gttgagggca gatataaaca
    44941 cgataaggat aagttttatg taaaaaattt taatgtgttt tcaatagagg atattgcaca
    45001 agatttttct aaggaaaagt atgctctatt gagatacgca ttagaaaatg ttaagttaaa
    45061 atcaagacat gtagtttttt gtttaaatac aagagatgta attttaaaac cccaaaaact
    45121 tcctaatatg gacaaaaagg atttagacgg atttatgaag ctagagatgg acgaaatgat
    45181 ggctttggag tcagatgaat atgtattttc gtatgaagta tctagtgaaa cacctgataa
    45241 ttcagatggc gatgaatcaa gcttaaatct tattattgga gctatacaaa aggaagaggt
    45301 aaatgcaatt gtagagataa tgcatgaatt tgatttagta ttagatagaa tagatacatt
    45361 atcaacagct tacttaagaa tgcttaaaaa tttagactac aatgatataa tggtaaccaa
    45421 tataagtgac aatagcacaa ttataaatat atataagaaa gatactttat ttattagtga
    45481 taatatacct ataaagataa ctgctaaaga tatggacatg caattattaa atgtagtaga
    45541 agaatcaaaa ggtcttatga attactattc gtcaagaaac tttggtaagg ttacggatac
    45601 tatattaata ttaggaaaaa gatattccaa taaattaatt tatgaaactt ttagctctgc
    45661 attttcaagc tatgtttctc aaggccttac tgagataatg gatatatctg atatgataac
    45721 tggagatata gatgaagatg atataaattc aattgtagag cttttaggaa gtatgataga
    45781 acctaaaggg aaaagagatt ttgaaaatat aaacttttta ccaatggata tacttagaaa
    45841 acagataaaa gctaaacaaa tgaaaaacat actgaaaatt gctccaatag ttattttgat
    45901 tcttgctata ccttatttat gtttaatttt ttcaaataat ttagcaaata aggaacttga
    45961 aaatgttaat ggtgaaataa gaaatattga atctgcatat tatcagattg gaaatataga
    46021 aaaaatgata cagagtaaaa gcgaagaaat aaaggtatat gatatgttaa ttgggaaaaa
    46081 ggtaaagtgg gaaaatttat tggatgatat agataaaaat actccaagta aagttgaact
    46141 aacaagttta tcaacaactt atcaaaatct tggaactaat aataaagctg ataattcaaa
    46201 aagtaataag actacaacag ataaatcaag taacaatgaa acaaaatcaa gtacgaaaag
    46261 tgatagtaat ggagataaaa cagataatga tttaaatgaa gataagacta caaatgtgtc
    46321 agaagcatta actggaaata gtgagactaa tagtaataac acggataaag atgataaaac
    46381 tccattgtat gataagatac caaatgttat aaatatagaa ggaaatgcta aagattcctc
    46441 ttatgtaggt caatttttat ataaattaaa gggattatac tatttcaagg atgttaaatt
    46501 acatagcata aaaagtaatg cagaagattc taactataca tttagtatga cattatacct
    46561 gaaagaaggt gtattaacta atgaataaag atatatttaa aaaagatata aaagatatat
    46621 ttaaaatgga agttagtcta aaagggattc ttataaaggt tataatttta ttagctattt
    46681 tatcatatgg ttattttgga ctttaccctc aatatcagaa atatattgct acaaaaaatt
    46741 ctgtaaaaca ggctactgaa aaattaaata tatataaaga gaaaactgca cttatgccaa
    46801 aatatgaaaa acaacttgag gaagttgaaa gtgaattaaa agaaaaaagc aaaaatcttt
    46861 tatatgatat gcaagatggt ctttttttaa taggtttaga taaaaaaatg aaggcttata
    46921 atattgactt aaccagttat gacgttggaa atgttaaaga atacgaacat ttttatggta
    46981 tatcaacaac aataagtgtt agaggtgatt atagaaatgt caaaaaatta atggcttatt
    47041 tagaagaaca aaaaaatgtt actcaaatac tagattatac aatgacttca tatatagaac
    47101 cagaaaaaga agaggaagaa acaacaaaca ataaggattt aattaaaaat gtgaacataa
    47161 ataaagatac aaaaatatac tgggtggaag gcgaaaataa gtggcattta tataaggatt
    47221 gtccattgat aaaagaggga gagattataa aaaatgacaa acttttagat aggtctacta
    47281 attatctatg taatacttgt gtacttagag ctgcaacaga aaaagaagca aaaaaagaag
    47341 tcgtagctcc aaaggcagaa ggtgtagtag atgctacatt taaatttata atgtacacta
    47401 aagagaatac tacaatgata cttgatacag ataatccatc ttcatggaga ccaggaaaat
    47461 ataatccatt taagtctagt gcgaaataaa gctaagtaag ctttaaagat aggtgatgag
    47521 tttgtacaaa aataatgaaa aaggtcttac tttattagaa gtaataatag ctgtttttat
    47581 actgacaata gttttaagta tttcttataa agtgttcaat ggtataacat cagcagtaaa
    47641 aaagcaacag attattacag atgctcaagt aaatattaat ctaattaata agtatctaaa
    47701 tagagatttg gaaaactgta aagagctaac taaaactggt tcaggtaata attatgaata
    47761 caatatagag atgccagata atgttgtaaa atatgaagtt tctatagaaa ctaaaaaaaa
    47821 tacagaggta tattctgtaa cgagaatcca aaacaataca attgatacag aaaatgaagt
    47881 tagagaagag ataatctata atcaaccact agttcaaaat aataaagaaa tgaaggaaac
    47941 accatttaaa atagaaaagc aaactggtaa atctatttat actgtaagta tatactataa
    48001 tgaatcggtg caagaaagtc ataaaaatag caatttaaat aacaaaactt atacatttga
    48061 tgttatgtca agaatagggt aataagaatt tattaaaaaa taagtaggtt gataaaatat
    48121 gagtaaaaag aatagtgaaa gaggattttc cttaattgaa gtgttagtag ctatggctat
    48181 tatgggaatt gtactatttg cattttttaa tattatcaat actaataaca aagcaaacac
    48241 taaaaatgac acagatataa cttccttaaa ctatgtccaa agtgaaatag aaaatctaag
    48301 agaaaagata aaaagtggag aatttgattt tgatagttta gataaactag aagatggaac
    48361 tgtcgtatat gaaaaattaa tagataaatc aaaaaaagtt gtatatgata aagttcttag
    48421 tgaaggtgat gtgagcttat atgatactcc atatgaaaag attacaacaa taaaagatga
    48481 agatggtaat ttaatcgaca aaggaaatat tacaaataag ataaagacta ttgtagaaga
    48541 taaaagtggt cagatatata aaatagctgt aactgggaaa agcatgaatg actattcaag
    48601 taaaaaagag gttaaaattg taactgaaat atttaaagat aaataatttt acttgggggt
    48661 gagattttga ggaagtggaa taaatttaaa agtgaaagag gggcagcttt agttttagtt
    48721 ttggttgtag tagcattgct ttctattgtt ggattgatat tttcaaatca gatagctaat
    48781 agaataaaat ctacaaagac tactaatgaa ggtatacaag caaagtattt agcagagaca
    48841 tgtgtggaaa atagtattga taaagcatat gaaaaattgt atgatgagct tgagaaaatg
    48901 gataatgagt ttaaaagtga aaatcaagaa aaatccatat ctaggtcaaa attaagaaat
    48961 atatcagatg aagattttaa taatcaagat gaggaaaata ttgaagcaga aagacttgga
    49021 tatatgaata atataaattt ctatttaaat aaagcttctt cagacttgga aaaagctagt
    49081 atggaattga aaaagctata tgatttagat atgttggact atagagacat tgaatatgta
    49141 gatgcaaata ttatttcaca tagaaattct atcttagaaa tttgcaagaa ttatacttct
    49201 ggagatatta gtaaaataaa tgaatatatt ctgaaagaag atatagactc aactacattg
    49261 attgaggcaa agttagtgaa taatgatatt cttttaaaaa agtttttaga agaaaataaa
    49321 attgaaaatg aacatttaaa ttcagccttt tcacatactt ataaggcatt agataacatt
    49381 tctttggcga tgcaaaatat gattgagtat agacatacgt ttcatataga tgagccaaaa
    49441 gtagaagtat ctaatgggat tccagactct caacaatatt atgaactaat acaaaatcct
    49501 ataataaata gtatggagta tagctggaat agtaaatggg atacattaga aaacctattg
    49561 gaaattttac caaatcaaac tcaaggattt aattctttaa gagttcattt gagaaataat
    49621 gtaagaaaat ttgagaagtt aagcgataat atatctagtg gtaaaaaaaa tacagcaaaa
    49681 aatttcttaa agtataaaga gttattgtat gaaatatcag accaatgtaa tcaactaaaa
    49741 agtatgtctt atgagaaaat acctgttaaa tatgataaca tggctttgat aacaactttt
    49801 gactatatac aaaatgagct tttagcagag ataaaatgca gattaaaaga attaaaacca
    49861 caagaaattg ataaaacaga aggtattact ataaaaatac ctttttataa ggctgactac
    49921 gacatgacaa aagaaggatg gcctaaatta aaagaaaatg gttcaggggc ggagttaagc
    49981 ttaatggtaa ctggtgataa aaatggaata aaagaagtag aagttacaga tggtaaaaaa
    50041 aatattattg gtttaggtgt tgaagaaaat tcaaattcaa agtataaggt tgacgccatt
    50101 gtaaacttta atctaaatat agatacaaat gtagttggaa attatgatat aaaagataaa
    50161 attttgatta atcatgatat aagttcatac aaaaaagtaa actaaatata agaagcaact
    50221 tagtataaat aaataataaa ataattttat tatttactac taaaaaaata agtaaactaa
    50281 tagaagatat ggagagtaaa tattatgtat atttatgatt tattagagca aggtataaga
    50341 ctaaacgcat cagatataca cataacagtt ggaacaaatc ctgtagctag agtaaaaggt
    50401 ggatttgtaa aactaagtga acaaatattg acatcagaag taacaatgca aatggcaaaa
    50461 gacatagcag gggaatctat gtttaaagta atagaagaac atggagaagc tgatttttca
    50521 gcttctctaa aaacaggaga aagatttagg gtaaatgcat ataggcaaaa aggaaattac
    50581 gcaatagcaa taaggactat aacagcagaa attccaacat ttgaaaaatt aggccttcca
    50641 gagagtataa aatcttttac tgaaaagcat aaaggattag ttttagttac aggtccaaca
    50701 ggaagtggta aaagtacaac acttgcaagt atgataaata taataaatga aaaacaacaa
    50761 aaacatataa ttacacttga agacccgata gaatatgtac atcatcataa acaaagttta
    50821 gtaaatcaaa gagaagtagg aactgataca gaaagttttc attcagccct tagagctatt
    50881 ttgcgtcagg acccagatgt aatactaata ggagagatga gagaccctga aactgtttca
    50941 atagcattaa cagcagcaga aacaggtcat ttagtatttt caacactaca tacagtaggg
    51001 gcagctaaga caattgatag aatagtagac atgtttcaac catcccaaca gcaacaaata
    51061 aaaacacagt tatctacagt ttgtgaaggt gtggtatcac agcagttact tcctacagca
    51121 gatgggaaag gcagaatagc agcaattgaa ttgatgtttg ctactccagc aataaaaaac
    51181 ctgataagag aaggcaaaac atatcaaatt ccaaacatga tacaaactgg agttaaagta
    51241 ggtatgaaaa ctatggacca agatttaatg gaactttata aaaatggaaa gattacaaaa
    51301 gatatggcac ttagtaggtg tactgaccaa gagtttatga caagaatgat aggtggagta
    51361 aactacaatg gatattataa tcgttaatct atatgtattt atagtgggta tagtgtttgg
    51421 aagtttcttt aacgtctgta tttttaggat tccagaaggt atatctattt ctagaccacc
    51481 ttctcattgt atgagttgta atactagact aaagccaata gacttaatac cagtgctaag
    51541 ctatttaatg agtggcagaa agtgtagata ttgttatgag aaaatttcta gtagatatgc
    51601 tatagtggaa ttaattacag gacttttatt tttaacagta tttatgttat atggaatatc
    51661 tacatcaact atttattatt tggttcttgt gtctttactg attataatta catttataga
    51721 tattgaccac tttataatac cagataaaat attggttttt ggagctatat tttctatgat
    51781 ttttaacctt ttatttaaag aaattccagt taaggattca attctaggtg tttttatttg
    51841 tggaggaagt gtctggatta tagttctttt gattgaattt gtaataaaaa aagagtgtat
    51901 gggtggagga gatatcaaac tatttgcaat gctaggtttt tacatgggtg ttaaaaatgg
    51961 tcttttgaca gccttactaa gcgtttatgt gggagcagtc tatggaatct gtgtaataat
    52021 tataagtaga ataaaaggaa aggaatataa ttctgttata ccatatggac cttttatatc
    52081 tattggagca ttaataacaa tcttatctgg aaagcaatta cttgaacttt atttttctat
    52141 ggttatatag atgatagtca agttaaataa agttttaatt taaaaaaatg aattaaagta
    52201 tttaataagg acaatattat tattaaccct attactaaag tgctacaaac tatggagtat
    52261 ataataataa tttccttatt ttttgcacat ttgataaata aatctctatg tgttttttgt
    52321 gatagagaaa agaaaaattt atataatacg ttttttattt acataataac tgtagtttta
    52381 tctgtattaa tatatctaaa atttgggatt agtgtagaat gtgtacaata tatggtttta
    52441 attccatttt taataattat atccattgta gattactaca caatatatgt atatgatata
    52501 acaatagtta gtggtataat agttcaaggt ataatttttt tggccttttt caatatagtt
    52561 caatttgatt atataaatca tatatttgga ctattgatag gctttattat accttatatt
    52621 attgctaaac taacaaaagg attaggctat ggagatgtag gtctctatag cttatgttgc
    52681 tttgcattgg gaaataatta tgggttttat gtgatagttt tatcatttat tttagcgttt
    52741 ttgtttttta ttattttata cataactaaa atatttaaag atgtagatat aagaaagcaa
    52801 attcctttta caccttttat ttccttgtca acaattttaa taatatttac agagtataat
    52861 atatttaatt tttattataa tttgttatac aaatttatat aacaggaggt aagaaatgta
    52921 cgtagtagta gggttaggta atccaggaaa gaagtatgag aagactagac ataacgttgg
    52981 atttgatgtt atagatattc ttgctaaaga atacaatatt agtgttacta aaataaagca
    53041 taaagctttg attggtgaag ggagagtagg tactgaaaaa gtattattag ttaaaccaca
    53101 aacttatatg aatttaagtg gagaaacatt aatagatata tataaatatt ataaggttga
    53161 tttaagtaat atagtagtag tatatgatga tatagattta gaagttggaa agatacgtat
    53221 aagaaaaaaa ggaagtggag gaactcataa tggcatgaaa tctataacta agtgcctagg
    53281 ttcaaatgac tttccaagag tgagagtagg tgtatctaaa ccagaagctg gtcaagactt
    53341 agcagacttt gtactttcta gatttagaaa agaagaatct gataacataa atgaagcatt
    53401 agaaaaagcg gcagatgcta ttgactcaat aataagagaa aatatagata tgtctatgaa
    53461 caaatataat ggatagtgag agggagattt aaatgaatga tgttttttta tatcctttgc
    53521 aaaattcaaa agagtataaa gatataataa actgtataaa aaatacaaaa ggctcattat
    53581 tggttaatgg acttttacca gtacaaaaac ctcatatatc gtattctata tttaatgatt
    53641 tatcacgaca aatgattttt ataacgagta gtgatttaga agcaaagaaa gtatacgaag
    53701 atttaagttt ttatatggaa gataaagttg aatatttagg ttttcaagat atttattttt
    53761 atcacttgga tgcaaaggac agaaacgaag aagcgaagaa attaaaagta ttgttaaaac
    53821 tagcaaataa agaaaaaatc attttagtaa cttctattga ggcagtttta agaaaataca
    53881 taccaaaaca agttttgctc gatagtgtat ctcattataa ggtgggagat agcttagact
    53941 tagaaaagtt aactgagaaa ttagtttcat tgggatatga aagagtttca aaaatagagg
    54001 ggtttggtca atttagtata agaggtggaa ttattgatgt attttcactt gaatatacaa
    54061 atccaattcg tatggaatta tttgatgatg agatagattc catacgaact tttgacgtat
    54121 attcacaaaa atcaattgat aaattgcaac aattctctat aactccatct agggaattta
    54181 tttatcccga aaaaactaca gatgcattag ttaaattaaa aaaagaaaca actaaaaata
    54241 ctgatgaagg tacatttcaa aatatagatt atatatcaag taagacttat tttgaaggtg
    54301 tagagaacta tatagattat atttatccag aagaaaacaa aagtatattt acttatttag
    54361 cagatgatgc aatagtattt attaatgata taacgagact taaagaaaga tgcgaaaatt
    54421 atataaatga gtttagagaa aattataaat taaatcttga aagaggtctg gctattaaaa
    54481 accaaggtaa attgttatat cactatacag atttagagta cttagtaaaa gacaagtctg
    54541 tggtattaaa ctcgctattg ccaaagtcta taaataactt ttctataaaa tctataataa
    54601 attttgaaag tagagaagtt cctacattta atggaaaggt agatttgctt gtagaagaat
    54661 taaatcggct gaaatataat ggatacaaaa taattttagc aacaaatacc ttagaaagag
    54721 caaataaatt aggaaaagat ttattggaca aaggcttaga aacgaccatt tctaaagata
    54781 gagatattga aattaaatct tcacaagtta taattgtacc tgcacacata aacagtggtt
    54841 ttcagtataa atctataaag tttgttgtta taacagataa cgaaatgata ggtgtttata
    54901 aaagagcgtc taagactagc aataaaaaag ttaaaaaggg aaagaaaata gaatcctttt
    54961 tagatttaag tgttggagac tatgtagtcc atgaaaatag tggtgtaggt agatacacag
    55021 gaattgaaca aattacagtc aatgctatta aaaaagatta tatgaaaata gtatatcaag
    55081 gtggagataa tctatatgtg ccaatagacc agatggacaa agtacaaaaa tatattggtg
    55141 cagaagtaga aaaagttaag ttaaataagc ttggaacaaa tgagtggacg aaagcaaaag
    55201 caaaagttaa aaaagaaatt gaagatatga caaaagactt gattgagctt tatgcaaaac
    55261 gtgaaaaaat acaagggtat aagttttcaa aagatacacc ttggcaggca gagtttgaat
    55321 cgttgtttcc atatcaagaa acagaagacc aattaaaggc tatagaagaa actaaaaaag
    55381 atatggaatc aagtaaggta atggataggc tagtatgtgg agatgttggt tatggaaaaa
    55441 cagaggtagc tattagaagt atttttaaag cttgtatgga ccaaaaacaa gttgctgtat
    55501 tggttccaac aacaatattg gcccaacaac attataatac attcaaagag aggtttgaaa
    55561 attatccttt gagagtagaa gttttaagta ggtttaaaac accaaaacaa caaaaacaga
    55621 ttattgaaga tgctaaaaaa ggattagtag atattttaat tggaacacat agaataatat
    55681 ccaaagatat aaatttacca aacttaggtc ttgtagtcat tgatgaagaa caaagatttg
    55741 gagtaaaaca taaagaagct ttaaagaaaa ttaaatctac agtagatgta cttacacttt
    55801 cagcaactcc tataccaaga acattacata tgtctttaag tggaataaga gacatgagtg
    55861 ttatagagga acctccacaa gaaagacatc ctgtaataac atatgttaca gagagtaagg
    55921 aaagcgtaat tcaagatgag atagagagag aattaagtag aggtggacag gtattttttg
    55981 tatacaatag agttgaacac attgaagaaa tggcaagtat gattcaaaaa ctcgtaccag
    56041 atgctagagt ggctgttgca catggaagaa tgacttctaa atcattagaa aatataatcc
    56101 taggattttt aaataaagat tatgatgtac tagtctgtac tacaataatt gaaactggta
    56161 tggacatatc aaatgcaaat acaatgatta tatatgatgc agataaaatg ggattggctc
    56221 aactttacca attgagaggt agggttggaa gaagttcaag acaaggatat gcatatcttt
    56281 tgtatgaaaa agataaaact ttaagtgaga tagcggagaa gagattaaaa gctatcagag
    56341 aatttacaga gtttggttct ggttttaaaa tagcaatgag agaccttgaa ataagaggag
    56401 caggaaatat tttaggttct caacaacatg gtcatatggc agttataggt tatgatttgt
    56461 acgttaaaat gcttaatgat gcaattaaaa aagtcaaagg tgaacctatt gtggaggaaa
    56521 ttgatgttga aatagattta agtgtaaatg catacattcc agataattat atcaaagatg
    56581 agttgattaa aattgaaatg tataagaaaa tagcttctat tgaaaacaaa gaagacatgt
    56641 tggatataca agaagaactt gaggatagat tctctgatat acctaaacca gtccagacac
    56701 ttcttacaat agcttatata aaatcgttat gtaagatatt aaaaatagaa aagataagac
    56761 aattaaaaga tgaaatatta ttagttccaa taacaaagta tagaactaaa cagaaaatag
    56821 gttataatat agttacagaa ttagaagaat tattagaaaa gatgtgcaag gtaaaataac
    56881 ttgtaaattt aagaagggat gttttaaatt tgaaaaaggt aataacattg gtaatagcta
    56941 tgattttggt agtatctgtg actgcttgca gttctagtaa aggagaaact gtggcaacag
    57001 ttgaaggtac aaagatatca tcagatgaat ttaaaaaaac tatagctctt tataaagatt
    57061 ctatggaaca aacttatgga aaagatatat gggataagga agttgaaaag ggtgtaaaat
    57121 acaaggacaa gtttaaagat ttaattcttg accaacttat aactacagaa gtaatatatt
    57181 ctcaagctaa aaaagataac ttacttccta aaaaagagga tgttgaaaaa agctttaaag
    57241 aattaaaaga cgctatgggt aaagatgaaa aatataaaga acaacttaag aaattaggta
    57301 tagatgatga gtttttaaga gaccaacaag agaaagattt agcaatgcaa aactatcaaa
    57361 gtaactttgc taaaaagact aaaatatcag atgaagaaat gaaaaaatac tatgatacac
    57421 ataaagatga atttaaaaaa gatgaggtag aagcttctca tattttattg aaaacagtag
    57481 atgataataa caagccactt tcagataagg aaaaagctga agctaagaaa aaggctgaag
    57541 aggctcttaa agaagttaaa tcaggtgagg attttgcaaa agtagcaaaa aaatattcac
    57601 aagatacttc agcaagtgat ggtggtaaat taggattctt ttcaaggggt caaatggttg
    57661 ctgaatttga agatgctgct ttttctatga aaaagggtga agtatctgat ttagttgaaa
    57721 ctcaatatgg ataccacata attaaagtga cagacagaat aaatgagcaa acatcatttg
    57781 aagatgctaa agaaactata aaagaccaac tgttgaaaaa taaatatcaa gaacaaatag
    57841 aaaaattaac taaagaagct aaggtagaaa aagatgaaaa agtaataaat aaaatcacaa
    57901 tctaagactt aaaaaaagag taatatgctt aaaaaagcta tatctggata agatatagct
    57961 ttttttgtat atttttctta acaatggtaa aaataaactt aaagtaattt atggagggat
    58021 taaaaaatat gaaagcaaca ggtatagtta gaagaataga tgatcttgga agggtagtta
    58081 tcccaaaaga gataagaaag acattaagaa ttagagaagg agacccttta gagatattca
    58141 cagcaaaaga tggagaagtc atacttaaaa aatactctcc aataggtgaa ttaaatgaat
    58201 tttcgcaaga atatgctgaa acattagctg aagtactagg atatggagtg ttgataacag
    58261 acttagattc tataatagct gtgtctaagc ttcctaaaaa agattataaa gaaaaaagta
    58321 taagtgatga gttagaagca ataataagtg aaagaacagt aaaatattca aaagataaaa
    58381 acattttacc attattcaag gaagaccaaa aagaatatac tagtcaagtt atcatgccta
    58441 taattagtgc atctggtgac tgtataggtt caattgtagt tgtttcaaag gataaagata
    58501 caattgcaga aagtgaagaa aaatctttaa aaattgctgc taacttttta ggaaaacaag
    58561 ttcaataatt ttatcataaa gaccttttac atgaaggtct tttgaagttt ttgtgtaaaa
    58621 tattgaatat agcattaaaa atatggtata ttaaagtttg ttatataaac agtatggagg
    58681 ttatgttatg aacaataaca aaaataaaga ctcctttcta aaaggagccc ttatacttgg
    58741 gctagcaggt gtaatcgtaa aaataatggg tggatttttt aggattcccc ttggaaacat
    58801 gattggttca gaaggaatgg gatactatca ggctgcttat cctgtgtata ctctattttt
    58861 aacattagct actgctggat ttccaacagc tgtggcaaaa ttggtttcgg aaaaagttgc
    58921 aattggaaat tttaaaggtg caaataagat atttaaggta tcacatacag ttttattcat
    58981 taccggtata atatcatttt gtatattatt ttttggagcc gactacatag taacaaacgt
    59041 aatgaaaaat ccaggtgcgt tatactctat gaaagccatt gctccagcat tactatttgt
    59101 tcctgcaatg tctgcatata gaggatattt tcaagggaga caagatatga ctaagattgc
    59161 tgtgtctcaa gttgcagagc aattttttag agttgtatta ggtcttactt tggcttactt
    59221 gcttatgaag agtctaggtc agacttttgg tgcagcaggt gctatatcag gggctacaat
    59281 aggttcaata gcatctatgc tttatttggt gtttgcttat atgctaggga gaaaagagcg
    59341 tagagcagag atagatgcaa gccaaagatt taaagatgaa agagtgtctt atatttttaa
    59401 aaagctttta actgttgcca ttcctataac aataggagca tcagtaatgc cacttgtaaa
    59461 tatgatagat aatgttattg taataagaag acttatggaa gcaggcttta catacaaagt
    59521 ggcaaattcc atgtttggtc aattaacagg tatggcaatg gccacaataa atttaccagc
    59581 agtcataaca actgcaatga gtatgagctt agttccagca atttctaaag catatgcatt
    59641 aggaaataag tcaaaagcta gaaaagatac taagagtgca gttaaggtaa ctttacttat
    59701 agtattgcct tgtgcgtttg gtatggcttc tttagcaata ccaataatgg gtcttttatt
    59761 tccacatgaa ccatcttctg taggaactat tttgtttact cttactccat gtgtattatt
    59821 tttaggatta atacagacat tgactggtat aattcaggga atgggaaaac caatagtacc
    59881 tgtaattgct ttatgtgttg gtatgctatg taaaattgta ataagttata ctttaacagg
    59941 tataccagat attaatgtac tgggttcagc atttggtaca gttacagcat actttgtggc
    60001 agccatgatt aacttactat atgttaaaaa acacatgaat gtaaactttt ctaaaaaaga
    60061 atttataata aagccattta taacagttat gacaatgttt ataatggtta agttgagtta
    60121 tggtgcttta gttggattct taggaaacag catatctaca ataattgcaa tatgtgttgg
    60181 tggaattgta tatgtagtag taatacttgg aataggtgga attaaaaaag aagagattct
    60241 aactatgcca aaaggtgata aattatataa attgttaaaa aaagttaaac ttataagata
    60301 atataggaat atatatgcaa ataaaggaaa tactatacaa atagtaaatc tccttatttg
    60361 tataataata taaaaataaa gtgaaatttg ctaataggag tgatgttatg ggtaagataa
    60421 gtattgtagg tcttggacca ggagactatt ctcttataag ccaaggtgtg ttagaatctt
    60481 taataaatag ccctaaagtt ttttttagaa cagagaaaca tccaataatt gataaactta
    60541 aagaaactat acaatacact tctttagact acttttatga aaaagaggaa aattttgata
    60601 atgtttattt gaaaatatct gaatttatca tagaagaatc agcaaagggt gacctagtgt
    60661 atgctgttcc tggtcatcca agagttgcag agaagactgt aagtataata gagagattag
    60721 ccaatgaaaa gcacatagca gtagaaatat tgccttctat gagctttgta gacgctatgt
    60781 ttaactattt ggctatagac ccatctgagg gatttaaact ggtagatgct tttgaaatag
    60841 aaaattcata tatagacttg gatacaagta tgataataac tcaggtatat gataaattta
    60901 tagcatctaa tgtaaagtta aaacttatgg aacattatga ctataatcaa gaagtatgta
    60961 tagtaaatgg agctggtgtc aaagatttag aaagtaaaaa gtttataaag ctttgtgaat
    61021 tagacagaaa tgaaaacttt tttgactatt taactagctt atatataccc aaaagttcta
    61081 aaaagatgta taatacagta catgacttag aaataatagt taatacactt agaagtccat
    61141 ctggatgtga atgggataaa aaacaaacac atcaatcatt aaaaaattct gttatagagg
    61201 aagcatatga actttgtaat gcaattgata ataatgatat agatgaaatg gtagaagagt
    61261 taggtgatgt tcttttacag gttatattcc attgtcagat aggaaatgag gagggctact
    61321 ttgatttaaa agaagtagta aatggcatat gtaaaaaatt aattcataga catcctcata
    61381 tatttagtgg tgcaaaatta gatatgtcta agtttgagaa aacttgggaa gaattaaaga
    61441 aagaagagaa aggcgaaagt actataacag atggccttag aaggatacca aaacatttac
    61501 cagctcttat gaaggctagt aaagttcaac ataaagcagc acttgtcggc tttgattggg
    61561 acaatgtaga agatgtattt aaaaaaattg aagaagaata tcaggaatta cttgacgaac
    61621 acagggtcgg gaatataaaa tacataaagg aagagctagg cgatttatta ttttcgatag
    61681 taaatctagc tagattttta gacatagact caggagaagc cttgaactgt actacagaaa
    61741 aattcataaa tagatttgac tttatagaaa aaagtgcaat gaacttgaat aaaaaattag
    61801 aagacatgac acttgaagag atggatgagt tttggaatca ggctaaaaaa caataaaatt
    61861 gtggaaggaa atttgtcaaa aatctagaac ttttatcaca aagatagaag tctatattat
    61921 aaacattttt aggaggtaaa tatcgtgaat aaagctgaat tagtatcaaa gatggcagaa
    61981 aaaagtggat taacaaagaa ggaagcagaa gctgcgttaa acgcatttat gagttctgtt
    62041 caagatgcac tagtaaataa tgaaaaagtt caattagttg gatttggaac atttgagaca
    62101 agagaaagag ctgctagaca aggaagaaat ccaagagatc cagagcaagt tatagatata
    62161 ccagcttcta aagcaccagt tttcaaagct ggaaaaggat taaaggatat aataaatgga
    62221 taattagaaa tcttgtgaaa aagtgtggat aaatttgcca cactttttct atagaaccaa
    62281 ggaaggtgat aattatgaga ttagataaat ttttaaaagt atctaggatt ataaaaagaa
    62341 gaactgttgc taaagatgca tgtgataaag ggattgtcac tataaatgga aaagttgcta
    62401 aatcttcttc agaggtgaat attggagata cattagaaat acaatttgga gagaagaaga
    62461 tgaagtttaa gataaatgaa gtaaaagagc atgtattaaa aaatgatgca aaagaaatgt
    62521 acaaaatttt agaataaaat taatagtcct ctagtatatt aatagtagta ttattgaaat
    62581 atttactggg gggatattat ggaacagaac ataactttga aagatagatc aaagctggtt
    62641 atttcgggag tcgagcacat atactctttt aatgacaaaa gagtagagct caaaacttct
    62701 gtaggcgaga tggtaattga aggcgaaaac ctagatatga gcaagctaag tatagacgaa
    62761 aatataatta gcatagatgg aactataaat tctatggtat atgcaaagcc taaaaagcca
    62821 caagagagtt tcttgaaaaa ggtatttaga tagatgatac cttttactca agatgtaagc
    62881 attttttatg ctactattta tggtggaata ctcatagggg tgttgtttga tttttataga
    62941 gggctaagag ggaattttaa attcatcaat tattttgcta taatctttga tgtattgttt
    63001 tggtttctag ctacggttat aatattcgta acaataaacc ttacagaatt ttttgattta
    63061 agatattatc attttgtggc tttgtttatt ggttttattt tatactataa caccataagt
    63121 aaaattgttt tatcaattat caataaaata attcgttttg tgagaaattc ttttaaaaaa
    63181 gttacacatt acatagtcag ttttttaaat aatttgtatt acgttataat ctattcacta
    63241 cacctactat ttgatattat attttatata ccaaacatat ttatagcaac aagaaaaagt
    63301 ataaaaagaa aatcaaataa aaaattaaag aacaagaaaa aatcaaaacc taagaagaaa
    63361 aaaaataaaa ctaaaaaaag ggtgtaggaa aaatgaactt aagaaaaaaa ttttcaggac
    63421 agattattgt tatcagttta tttttaggaa tatcaatttt tagtatgatg acaggatttg
    63481 tatttgagta tacaaaagct aaggaatata aaaaagaaat agcaagtcta aataaacaat
    63541 taaaaaagac tgaaatacaa ataaacagct taaagaaaga tgaaaaatca tatgaaggag
    63601 acttagagga tatagcaaga aaaagattga atatggtaaa accaaatgaa acagtttatg
    63661 tagacataaa tcgttgatta aaacttgcta ataagtttac agatgtattg attttaaaat
    63721 ttaagattta ataaaaaagt cattcagtta aggatggctt tttgttttta ttgaggaggg
    63781 aatttcataa tgaaagtagg agctatagat attgggacaa actcaatgag attattgata
    63841 gcagattata gagatgggaa ttttataaac agaaaaaaat ttgttaatac aactagaatt
    63901 ggttatggtg tggatagtaa aggatatata aaagaagatg ctatcaaaag aaatataaaa
    63961 gcattaaaag aattttctga taagtgtaat gatgaaaagt gtgaaaaaat ttattgtatg
    64021 gggacatctg ctcttagaga cagtaaaaat ggagattact ttgttaaatt agctaaagaa
    64081 gaaactggaa tagatgttga tatagttacg ggaatagaag aatcaaattt gggctttaag
    64141 ggtgttttag aaggaataca aggtgatgat actattattg ttatagatat tggaggaggt
    64201 tcaacagaat ttattgtagg tgattcagaa ggaattaaat ttaataaaag tgagaatgta
    64261 ggtgctctta ggatgacaga gaggtttttg aagcacgata ttattgaaga tgaagagttt
    64321 gaagctatga gttcatttat atatgatgag attgacaaaa cgttaaatca tataaaagac
    64381 agaggattaa aaactcttat aggtatagga ggtacgatta catcattatc agcaatgaat
    64441 caagaattag agatttactc tatggaaaaa atacataata gtaaaataca caaaaaagaa
    64501 atagaagaaa ttctacaaaa attaataaag atgacattaa gtgataaaaa gaacttaaaa
    64561 gggctacaag aaaaacgtgc ggatattata actgctggtg taaaaatatt gaatataata
    64621 atggaaaaat tagaaataga agaaataacc gtaagtgaat acgataattt ggaaggctta
    64681 atatgtcaaa agtcaaaaaa aatgtattaa tattttttga tttttaaaac cgattatgac
    64741 aaaaaaaatt actcctcttt gttacaattc ttaatataaa caaaagagga ggttttttta
    64801 tgcaaagaag tgtagcagtt gtgaagaata agactgtaaa accaaatgtc ataaaattaa
    64861 taagcggata tattgatttt actacggcgg cattcgctgt gatgggattt ttacttagta
    64921 ggtctattat agtagactct gtagctcctt taggaattgc cttttttata tgtgcatcaa
    64981 agattgataa gtacaagata cctgtattca tatcaacatt attaggaata ttgttatcat
    65041 ctaatgattt tggaaatgta gcaaaatatg ctgtgtgcct tacaatattt atggctatga
    65101 ataaaaaatt aaaattatca gattcaattg ctaaggttgc atttataggt gcaataatac
    65161 tacttccaat ctccatggga caagctttct tttcaaatag gtatgtatat ggtcttctta
    65221 tagcaggagt agaagcaata ataatgttta tatctatata tgtgttttct tatgggatag
    65281 gtttaatggc caatgtcaac aataggatgt ctgtacggac agaagaagcc atatcaataa
    65341 gtttactgct aacctttagc ataatgggaa taggagatat atctctgttt ggaatatcaa
    65401 taaggaccat tttatcgacc gtattgatat tgctgacctc aatagtaggt ggagctacca
    65461 tgggagcatc tagtggtgta attattggtg tagcatccat tttaaataat atgacatcag
    65521 ctgtttatat ggggatatat tctttttcag gattaataag tggagcattt aataaaataa
    65581 ataaatattt ctgtatacta ggatatattc taagttggac aataatatac ctatatactt
    65641 ctggtattac ttctaatatg atgcaattaa gagatatact attaggttgt ttgatagtac
    65701 tagtattgcc agagagatta tttaataaga ttgaaaaatt aataaaaagt aatgtggctt
    65761 caaatgaaat cgtatacgat tatattatga gaagcaaaaa cctaactaat agcagattga
    65821 acagtattta taaaacttat gatgaccttg cagatacatt tgataagata agagaaaaag
    65881 acaaagtgct agaccaaaga gacatagcta atgtaataga tatgattcat aatgatgaat
    65941 gtaaaagttg tagtatgaga agaatgtgct gggagtcaag atttaatcat acatacacta
    66001 tgatatataa cattcttgaa aaaattgagg agaaagggga gttaagtcta aacgatatac
    66061 ctaaaaattt tagaaaggaa tgtatgaaac cagagtcaat agtgaaaata tcaaatcact
    66121 attacaaaat gtttgtatta gattatgatt ggagtgtgaa gttttcagaa agtagaaagt
    66181 taatagcaaa tcagataaga agtatttcaa aatccattaa atccttatct caagatttag
    66241 aaggcgacat aatgctagat atagaaaagg aaaaaaatat atatgaacag ttagaaagat
    66301 atgatataac agttgataaa gtaagttact taacaaaaag taacagtgag tttgaaatat
    66361 caatagaaaa gaaaacttgc catgatggat gtatgtgtga agataaaatt gtaaatataa
    66421 tttcagacct agtaggggaa aatatgtctg ttagaaaaat aggatgtcac tgtttaggag
    66481 gtaaatgcaa ggcaacattc gttaaatctc aaaaatataa ggctgttaca gaagtttcag
    66541 caatgtcaag ggatgggcat atcctttgtg gagacaatta cacatacatg gagataaatg
    66601 atggtaaata catgatggct ataagtgatg gtatgggaaa gggtaaaaaa gcatatgaag
    66661 aatcttctgc cactattgat atactggaaa aaatgataga tgcaaaaatt aaagatgaga
    66721 tagtcattga tactataaac aatatgttgt tgttaaaatc ttcagaagag atgttttcaa
    66781 ctctggattt gggaatttta gatttaaaac gaggttgttt agaaactata aagatgggag
    66841 cgtgttctac ttatataaaa agagaggatg gagaagttga cttaatatca tcatcatcat
    66901 tacctgtggg tatattatca gatgtaaaaa ttgatagaaa aaatgtaaaa gttaaggaag
    66961 gagactatgt tataatggtt tctgatggaa ttgtagatgc aggaagaaat aacaatttag
    67021 gagacaattg gttaatatac ttcttaaaga atatagagac tacaaatcca aaggaaattt
    67081 caaatttaat attggataga gcattagagt tacaagcgtt acaaatcgaa gatgacatga
    67141 ctgttttagt tacgaaaata tgtacaaaat aaggtttaca agattatgtg tataagatgt
    67201 ggataacttg tgtataatat gttaatagac ttttgaaaaa agtatagtta ttaacaaaaa
    67261 taacatataa atcaacaaaa tttgttgata agtatggcag aaatgttaat aaaatgtaaa
    67321 aaaagaaaaa acaaaagcta ttatcaaaag agattcatgt tgaaataact aaatttctaa
    67381 gtaaagaaaa cctgtgaata tgttgattat tatgtggata actattaatt cgatatttat
    67441 ttatatagta tgaataattg aataatatat ctaaaaaaag ccatcctatt attaagtaaa
    67501 cttattgaaa ggatggcttt ttatgaatag aaaattgtgc aaagcaataa tacctttatt
    67561 tatttctatt ttttgcatgt taataataag tataaaagct gaccaaggat atcaaaagaa
    67621 taatgacaga gcaattactg tcatgaataa taatgatact gaagatgatg ctgaaaaaaa
    67681 acaagaaata gactatgaaa aaacatgttg gaatttgcaa tctttgttta aaagtgatga
    67741 ccaatggaaa aaagaactta agagttttga taaagatact aaagagttga aaaattacat
    67801 aggtaaagta acaaaatcgc caacacattt gtcttttgcc ttgaacatta aagaaaaact
    67861 tgatataaga ctaaataaac tttctgccta tgtaaaatta aaacaagata ctaataaaaa
    67921 ttcgtataag tacttagata tgaatgatag tatgagtaaa tcctatggga attatatggg
    67981 gatttgttca gatttagagt tagaaatact aaaattatca aacaaagatt acaagaaaat
    68041 tatttcaaat aaaaatataa ataggaaata tggtgcatat ttaagagata taagaaggaa
    68101 taaagtacat tatttggatg ataaatcaga agatattcta agcaaagtat cttcaataag
    68161 tagtcttcca agtcaagtgt atgaattatt tagaaatatg gatagaaaaa ctaatttaac
    68221 accagctcaa tatgcgagtg aattagagtc atcagataga gaaaatagaa aaaaagctta
    68281 tcaagatgaa cttataactt acaatgataa tataaataca attgcaggtt taattactgg
    68341 tcaagttaaa aagaatattt tttattctca ggaaagaggt tatgagtctt ctttagaaat
    68401 gtacctggaa gctgataatg tagatgaaaa agtatacaat aatttgataa ggactgtaaa
    68461 tagtaatatg gacagtctac acaaatatat agatttaaga aaaaaagtat taaaattaga
    68521 taaggttcat tcatatgata tgtttgtgcc tatggttaag gctgttgata ataatattaa
    68581 ttatgaaaag gcacaaagta tagtatattc agcgttgtca cctttaggaa aagaatacaa
    68641 tgatgtagta tataaagctt tcaatgaaaa atggatagat gtttattcac atgaaaataa
    68701 agtaagtggt ggatactgct tatctgttta tgaaaatcat ccatatatac ttttaaatta
    68761 taataattca ttaggttcag tttcgacttt atcacatgag cttggacatg ctgtatatga
    68821 atacttaagt tctaagaatc agaattattt taattcaaat ccgtctatat ttactcacga
    68881 agttgcatct acaacgaatg aagcattact ttacgagatg cttataaaag aagctaaagg
    68941 caatgatgaa aaagcttatt atataacaca gtatttagac ttgataaaag atactcttta
    69001 tacacaaact atgtatgctg aatttgaaaa aactatacac gagttagtag aaaaaggaaa
    69061 aagtgtaaac gcattagtat taaatgatgt ttggggacaa cttcttaaaa aatattatgg
    69121 acaaagctat gaattagacc aattatctaa ggtcggatgg gctagaatcc cacattttta
    69181 taatagtttc tatgtctata aatatgctac aggttgtagt gcaggagtaa gttttgccca
    69241 agacatccta aagaatggtt cagataacta tatgagcttt cttaaaaagg gaagttcaga
    69301 ttatccaata aacttgttaa aatcaagtgg cataaattta actaatacaa aacccataga
    69361 aaatacaatc aaaaaatttg atagtttagt agcagaactt gaaaagctat tataaacatt
    69421 attgtaatcc acatggtaaa tatatcgaat taatgataaa atatactaaa agtttaaagt
    69481 cctatcaaga taggactttt tatttattaa ttagtgggag atgattctat gcaagttaaa
    69541 gttaaaaagg ctgtaatacc agcagcaggt ttaggaacta gatttttgcc agctacaaag
    69601 gcacaaccaa aagagatgtt accaatagtt gataagccaa ctctacaata cataatagaa
    69661 gaagctgtgg cctcaggaat agaggaaata ctaataataa caggtagaaa taaaaaatct
    69721 attgaggacc actttgataa atctgtagaa ttggaacttg atttagagaa aaaaggtaag
    69781 aaagaattgt tggaggtagt acaaaacata tctaacatga ttaacataca ttatataaga
    69841 caaaaagagc caaaagggtt gggagatgca atttattgtg cgaggcattt tataggtgat
    69901 gagccgtttg cagtaatgtt aggggatgat attgtagata atgatgtacc ttgtctaaaa
    69961 caactaacag atgcatatga ggaatataga actacaatat tgggtgttca aaaggtaaat
    70021 caagaagata ctaataaata tgggataata gaagcaaaaa atatagaagg tagagtttac
    70081 aaggtaaaag acatggtaga aaagccagag tcaggaaaag ctccttcaaa tattgctatt
    70141 ttaggtagat atataataac tcctgaaatc tttgatattc ttaaggattt accaccaggt
    70201 aaaggtggag aggtacaact tacagatgct ttaaaaatat tatctaagaa agaagctatg
    70261 tatgcataca attttgaagg caagagatat gatgttgggg acaagctagg atttttagaa
    70321 gctactgttg actttgctct aaagaaagaa gaccttaagg aagattttat aaaatatttg
    70381 aaacatgtat gtagtgaatt tgataaaaat aacaatgtgt tatacaataa tacagatgaa
    70441 aaaatagatt taattgaagt caatgaagct agagaacttg aaattcaaaa ataattaatg
    70501 aaaggtgatg tatggtggca aaatcaagtc agaaaagaat taatagaata atagctgttg
    70561 ggattgtggg aatcctcgta ataacgagta taacagctgt tttgagtatg attgcaacta
    70621 tgtaataaga taaatgacag cagttgatat acataaatat aaaaaccata tataacttga
    70681 tgtagtcaaa aacttacaat attttagtta tatatggttt ttagaattta taaatgtaaa
    70741 aaaaaatttt tataatagtt gcatatttta aaggaaaata ttattataag atataaatat
    70801 agtgataaat ttataaaaac gtgctaagat tggacttgaa atacagtaat atagtaggta
    70861 ataatgtttt aaattttatt tattgaccat aatatataaa taatgatatt attagactta
    70921 actgtttaaa taaaactttt ctttttcatg ccttcaattt ccaaaataag tatgtatata
    70981 aatgaatata tagatgagga ggattttttt gattttagat gataaagtat tagaaggatt
    71041 aggtagtaag accttagttg agctacgaga gatagctaaa gaattaaaaa taaaatcaat
    71101 tactacatat aagaaaaatg agttaattga gattataaat tccaaaagta aaaatgagga
    71161 aaaagtaaac gaagagacta aagaaaaaaa tgaagatatt aaagaagaca taaagaatag
    71221 aaaagaaaat atagagcgtg aaagtataga aaaagaagtt gaatataaga gccctaccaa
    71281 atcatacaac aagaaagaaa tagataccca aaatatttcc caaaaccaag aacatagaaa
    71341 tcaaagaaat gactataata agacaacgag tgtaaatgat agtaataagt catcaaatat
    71401 atctaataga atggtcagaa ataataataa aaactattat atgcctaaac aagttgatga
    71461 atctaagata gtagatgaat ttaacacatc aaaagaggat gaagtagtag gtgtacttga
    71521 aattctgcca gatggatttg gttttttaag aggttcaaat tatttgtcta ctgaaggaga
    71581 tgtttacgtt tcaccatctc aaataagaag atttaatatg aaaacaggag acaagattaa
    71641 gggaataact agacatccaa aaagtggaga gaaatttaga gcacttttat atgttcaaaa
    71701 aataaatgat gaaaaccctg acacagctat acaaagaaat gcgtttgaga cattaacacc
    71761 aatatttcca gaagaacgac ttacactgga aactaataga aatgaaatag ctacaagaat
    71821 tatagattta atttcaccta taggaaaagg acaaagagga ttgatagtag cacctccaaa
    71881 agctgggaaa acagttcttt taaaaagcgt tgcaaatagc atagctaaaa atcatccaaa
    71941 tgttgaactt atagtactcc ttatagatga aagaccagag gaagttactg atatgaaaga
    72001 atctattgaa ggagacgtta tatattctac ttttgaccaa gtatcaagtc atcatgtcaa
    72061 ggttgcagag atggtgttaa atagagctca aagattagtt gaacatggta aagatgtagt
    72121 aattttacta gatagtataa ctagattggc aagggcatat aatcttacaa tatctcctac
    72181 aggtagaact ttatctggag gtattgaccc tggtgcactt catggaccta aaaagttctt
    72241 tggtgcagca aggaatatta gacagggtgg ttctttgaca atattaggaa cagcattggt
    72301 tgaaactggt tctaggatgg atgatgtaat atttgaggaa tttaaaggta caggaaatat
    72361 ggagctacat ttagatagaa aattagcaga aaaaagaata ttccctgcaa tagatattta
    72421 caagtctggt acaagaagag atgatttatt gcttgatgat gaagaaaaga ctgctctttg
    72481 gagattaaga agagaaatga gtaataattc agtaatggaa attacagata aagtcattga
    72541 acttataaag agaactaagg acaataaaga gtttgtaaaa tcaataaaga atttgtaaaa
    72601 tcaataatac aaatgcaaat atggattata taaactaata gaaaaatcac ttgaatggca
    72661 taataagttg tgctataata atgtagttga taatgtaaaa agaaagttaa tataattaat
    72721 ttttttaaat aaagagaaga ggtgactata atgcaaaagg aaatacaacc aaaatacaat
    72781 ccagttgaag tgcgttgtgc ttgtgggaac acatttgttg ctggttcaac taaggacgaa
    72841 ataaaagttg aaatatgttc agagtgccat ccattctata caggaaaaca aaagaacata
    72901 gaaaaaggtg gaagaatcga caaattcaag aaaagattca aaatggacta atttttataa
    72961 aagtggagtt ggaggaagcc aactcctatt ttattacaaa aaaacattca tattctactt
    73021 taaattgtct ttttgtatta taataataaa tatgtaaaat tgagtattct aaatacaata
    73081 aaatgaggtg agtggatggc aaaacaagct gtaggtgggc aagctgttat tgaaggtgta
    73141 atgatgcagt caaaagacaa aagagcggta gctgttagaa aaagtgacgg agaaatagca
    73201 ctaaaagaag atagaataaa aagttgggta agagataaga atatagataa gatacctttt
    73261 gttagaggtt cttttgttat gatagataca atgattcaag gcataaaaag cttaaacttt
    73321 tcttcagagt tttttatgga agaagctgaa gaggataaat ttgatttatt tataaagaaa
    73381 atttttaaag ataaggcaaa tgatataatt ataatatttt cattggtcat agcaatgtta
    73441 ctttctgctg gattattcat ttttatacct acattggtag gaggagcttt ttctaaagta
    73501 atgcctaatg attttatgct taatttaata gaaggaatca taaggatagc tatattattt
    73561 gcatatatag tattgatttc acgaagtaag gatatagaaa gagtctttca atatcatgga
    73621 gcagaacata aatctatata ttgctatgaa aatgatttag aacttacagt agagaatgct
    73681 agaaaattta aaagattaca tcctagatgt ggtacaaatt tcctatttat agttatggct
    73741 gtatcaataa tattgtttgc attttttggt tggccaaatc caattcttag aatatttatg
    73801 agaattatat gtgtgcctat agtagcaggt ttatcttatg aggtaattag agttcttggg
    73861 aaatatgata atggatttac taaaattatt gcatatccag gaatgatgtt gcaatatttt
    73921 acaacaaaag agcctgatga tgagcaatta gaagttgctt tggaagcatt gaaagcagta
    73981 gtagattaga gaaaaggata tttgttatga cgataaaaga tataattatt aaatattcag
    74041 ataaactaaa agatataagt gatactccaa gattagatac agagttattg ttacaaaaaa
    74101 cattgggtgt tgatagatta tatattcact taaatctgaa taaagagcta actgaagaac
    74161 aaaaaacaaa atttatgggt tttgcagaag aaagattaaa tggaagacca atagcatata
    74221 tagttgaaaa tagggagttt atgggattag atttttttgt taaggaaggc gtattgattc
    74281 caagaccaga tacagaaact ttagtagaag aaataattga aatatgtaga gaaaaaaaag
    74341 atgtaagtat attagatata ggaactggtt ctggagctat tacaataagt ttagcaaagt
    74401 acattgaaaa ttctaaaatc atgtcttttg atatatcaga gacagcatta gaaatagcta
    74461 aaaaaaatgc tattataaat gaagttggtg aaaaaataaa gtatattaat tctgatttat
    74521 ttacagcaat aagtgatagt aacataaaat tcgatatcat agtttcaaat ccaccatata
    74581 ttaaaaaaca agatatagaa actctgcaca aacaagttaa agattatgaa ccatataatg
    74641 ctcttgaagg tggagaagat ggattagact tttatagaag aataacagaa caaggtaaga
    74701 aatatttaaa taagtgtgga atattagcat atgaagttgg tcataatcaa gctgaagatg
    74761 taataaatat tatgaaaagt aatggatata aaaaaatata cacaaagaag gatattcaag
    74821 gcattgatag agttgttata ggttacaata tatgatataa tcaacaattg atatgttaca
    74881 tttaatcgag gtgaaagtat gttaaaaaaa ttagaggtat tggaagatac atataaagac
    74941 ttaagtgaaa aaataggtga tccggatgta ataaatgatc aaaaagtttg gcagaaatat
    75001 ataaaagaac atgctgactt ggaacctatt gttatgaaat acagagagta taaaagcgtt
    75061 ttagatagta taaaagaatc aaaagaaatt ttacaagaag agtcagatga agaattaaga
    75121 gaattagcta aaatggagtt agctgagatg gaagaaaaag tagcacctct ggaggaagaa
    75181 ataaaaatac tattattgcc taaagaccct aatgatgata agaacgttat agttgaaatt
    75241 agaggtggag ctggaggaga tgaagcagct ctatttgctg gtgatttatt tagaatgtac
    75301 tcaaggtatg ctgaaagaag aagatggaaa atagaattat tgagtgcaag tgatactgga
    75361 gttggtggat ataaagaagt atcttttatg ataaaaggta aaggtgctta ttcaagatta
    75421 aagtatgaat ctggagttca tagagttcaa agaataccat caactgaatc tggtggaaga
    75481 atacacacat caacttctac tgttgcagta ttaccagaag ttgaagatgt agaggttgaa
    75541 ataaatccaa atgatttaag aatagatgtt ttccgttctt caggtaatgg aggacaaagt
    75601 gtcaatacta ctgactctgc tgttagggta acacatatac ctacaggtga agtagtatct
    75661 tgtcaagatg gaaaatcaca attaaaaaat aaagagcaag ctttaaaaat attaaaagct
    75721 agactttacg ataaagcatt ggcagaacaa cacaaagata tagcagcaga aagaaaaagc
    75781 caagtcggaa caggagacag gtcagaaaga ataagaacat ataatttccc tcaaggtaga
    75841 ataagtgacc atagaataaa tttaacttta tataagttag atgcattctt agatggagat
    75901 atagatgaaa tgatagatgc tttaattact gttgaccaaa cagaaaaaat gactgcaata
    75961 taagtaatgt tttaaagaag ctgtttcttc ctagaaatta ttttctattt tgaaacagct
    76021 attttgcatt ttaggtcatg ttatcatatt ctattgtata taattgttat atacaagtat
    76081 aggagggtta tatgatacta aaagtaacaa taattgggct tttggcaggt gttataggga
    76141 caggattagg aggagtaata tcagccattt ttaaaagaga ggtcgataaa tatctaagtt
    76201 tttttatggg attatctggt ggaataatgt tggctgtagt ggtgtttgat ttaatgaaag
    76261 aatccatgga caaaatgggg ataatcaata cagttatatt tacgtttgtt ggagctctca
    76321 taactatgta cataaaaaca aaattagatg tgtctggaaa tatggcatct ggatatctta
    76381 tattcataag tatattacta cataatttac cagaaggact agccattggt tcttctttta
    76441 tgtcaactga aagtttaggt attacacttg ctatagttat aggtctacat aatataccag
    76501 aagggttagc tatggcatta ggcttagttt gcaataaaat gaaactaagt aaagttattt
    76561 tgtttactgt aatagctgga ttacctatgg gtctaggaag ttttttaggt gtttattttg
    76621 gaggagtatt tacttctctt ataggagtat ttcttgctac agcaggaggt actatgatgt
    76681 atgttgtatt ggaagaaatt tttccacatt caaaaagtgt gtattgtata attggtttct
    76741 tgttaggtac aatgattgta aattatatat aaaattaaaa tgaaagcagg catacaaaag
    76801 atgaaaacta ttataagtaa tattgatata aataatattg attatgagga aataaaaata
    76861 caagcaaaac ttcttagaga aggtaaaaca gttatttttc caactgagac agtatatggg
    76921 ttgggtgcaa atgcattaga tgaaaatgca gtaaagaaga tttatgaagc taaaggaaga
    76981 cctagtgaca atccattgat agtccatata tatgaaaaag aagaagtata tgacttagcc
    77041 aaagatataa gtgataaagc taaacttgtt atagaaaagc tttggccagg acctataaca
    77101 attatattaa ataaaaaaga tataattcca tacaaaacta gtggagggct tgatacagtt
    77161 gcaataagga tgccttctaa tgttattgcc agggcaataa taaaagaagc tggtatacct
    77221 attgcagctc cttctgcaaa tatctcaggt cgcccatctc caacaaaagc aaaacatgta
    77281 tatgaagaga tgaatggaag agtggatgga atagttttag gaggagacag taattttgga
    77341 ttggagtcaa ctgtattgga tttaacagaa gagactccta tgatactgag accaggaagt
    77401 ataactaaag aagttttaga ggaacttctt ggagaagtaa agcttgaccc atcattaagt
    77461 aaaaaagaag ataatcaaaa agctaaagct cctggaatga aatataaaca ttattctcca
    77521 aatgcagatg tatatataat atctggaaaa agagaaaatg tagcaacaaa gattaatgat
    77581 atgataaaat ctaatagaga caaaggtcta aagacaggtg taatgtgtat tagtagaaac
    77641 cgagagtttt atgatggaga agtaatcgat ttaggaaact ctcttgaaga agttgcaagt
    77701 aatctgtttg atgctcttat agaaatggac aaaaagggtg ttgatgtcat atattcagaa
    77761 gagttcccta agactggagt tggacaagca attatgaata gactactgaa gtcagcagga
    77821 tacaaagtga taaaggctta agagggagtt gtaaaatatg aatatactta ttgtgtgtac
    77881 aggtaatact tgtagaagtc ctatggctga ggctatacta aggaaagcaa ttaaagaatc
    77941 tggaagaagt attgaagaat attctatatc atcagcaggt atatctacag caaatggaat
    78001 gggtgcttct gaaaattcta tagaagtact aaaagaaatc ggtatagact tatcaaatca
    78061 tagaagtaaa gtaattacta aaaaattaat tgatgaatca gatataatac taacaatgac
    78121 taaatctcat aaagaaattt tagtacaagc agttccaaaa tgcaaagaaa aagtgtatac
    78181 ttttaaaggg tttgcaaata aaaatgagga agatatatct gacccatttg gtggaaattt
    78241 ggatatttat agaagcacta tgagagaaat aatgtattct gtcaacgaaa tagtaaaaaa
    78301 aatatgaaac ggaggataaa caaatgaaga taggattagg ctgtgaccat ggaggatata
    78361 atttaaaaaa agaaataata agctatttag aaagtaaagg catagaatgt gtagattatg
    78421 gaactaataa tgctacagat tctgttgatt atcctgttta tggagaaata gttgcaaatt
    78481 cagttataaa taaagaagta gattatggaa tattatgttg tggtacaggg ataggaatat
    78541 ctttagctgc taataaggtt cctgggataa gatgtgcagt tgtatcagat gtattttctg
    78601 caaaaatgtc aaaagcacat aatgatgcaa atatgttatc attaggcgaa agagttttag
    78661 gaaaaggtct agcacttgag atagttgaag catggattaa tacggatttt gaaggtgata
    78721 gacatgctag aagagtaaac atgattaaat caatagaaga aaaacataat aaataataaa
    78781 aattacagtt ttatcaggag gaattatcaa aatgagcaaa gtggtagaga caaaccatcc
    78841 attaatacaa cataaattaa ctttaatgag agataaaaat actggttcta aagactttag
    78901 agagctttta actgaaattg caatgttaat ggggtatgaa ataacaaaag atataccatt
    78961 aaaagatgta gaaatagaaa ctcctattca aaaaacatca tctaaagtag ttgcaggtaa
    79021 aaaattagca ataattccaa ttttaagagc aggtcttgga atggtagatg gattagtgag
    79081 cttaatgcca gcagcaaaag ttggacatgt aggactatat agagacccag aaacattaaa
    79141 accagttgaa tattattgta agcttcctca agacatagga gaaagagata taatagtagt
    79201 tgaccctatg cttgcaacag ggggttctgc tgtagcagct atagatttac ttaagtctaa
    79261 aggagctaaa agtataaaat tagctaattt agtagctgca ccagaaggta tagcagaagt
    79321 gcaaaaatat catgatgatg ttgatatata tgttgcatca gttgacgaaa gattaaatga
    79381 acatggatat ataattcctg gtcttggtga tgctggagat agattgtttg gaactaaata
    79441 aaaataaata actataaata tttattaggg actttcttat atagtgtata taattttatg
    79501 taagaaggtt cttttttata tattttttct caaaatagga attattgaat tgataaattt
    79561 ctcgaattga gaataaaaag tttttaccaa ttaaaaaaga ttgcttattt tacaaggttt
    79621 tatagaatag tagtctattt taatgtggca tgttagttgc ttacgattaa gattataggt
    79681 attaagtgta ttagttaaat tgagaattga atattaataa tattattggg agtggtaata
    79741 catgaagaat tgtatagtag taagaggggc tggagattta gcaacagcag ttattcataa
    79801 attacacaac agtggtttta aagtagttgc gttagaaata gaagagccat tagctataag
    79861 aaggcaaatt tctttttgtg aggctgttta tgaaaaaaaa gtaactgttg aaggtgtaga
    79921 atgtatattt tgcaatactg aaaaagaaat agaagaagtt ttatctcaac ataaagtagc
    79981 cttgattata gacccaaaag gagaaaaatt atctaaatta aatccaatcg ttttggttga
    80041 tggaatactc gctaagaaaa acttaggtac aactaaagat atggcaccac ttacaatagc
    80101 tttaggtcct ggattttgtg caggagaaga tgtagatgtt gttatagaga caatgagagg
    80161 tcataatcta ggaagaataa taactaatgg tagagcagaa aaaaatacag gagtaccagg
    80221 agcaataaaa ggtttttcta aagagagagt tatgcattca cagtatgatg gcataatgga
    80281 aaatgtatgt aagataggtg atatagttga aaagggagac ctactttgct atattaataa
    80341 ttatgaaaaa aaacatgaag ttagagcaac aatatctgga gtccttagag gtttattaaa
    80401 ggatggagca aaggtaaaca aaaatttaaa aatactagac atagacccta gaaaagaaga
    80461 agttaagaat gcttttacaa tatcagataa agctagatgt atagctggag gggttttaga
    80521 agccatacta aacaatggga tttatcccca aaattaattt atattagata aagctattaa
    80581 tattttatta agatgatata ataaatttaa tagattaaag agagcatatg acgggtattt
    80641 aaaatttaaa tggtaaaaat gctctttttt tattggacat tcaattaaca tgtgtatata
    80701 atataaaaat aagtatttat ttaggttgaa atctaataaa tatataatac cagtacataa
    80761 gcaaagagga gaaaagtaaa ttatgagacc atcatgggat gaatacttca tggaaattgc
    80821 agaagtagtg aaaaagcgtt caacttgtat tagaagacaa gtaggagctg taattgtaag
    80881 agacaaacaa atattaacaa ctggatataa tggttctccg agaaatcttg aacactgtga
    80941 aaatattgga tgtaagaggc aggaattaaa tattccctca ggagaaaggc atgaattatg
    81001 cagagcatta catgctgaac aaaatgcgat aatacaagct gctcataatg gtattagtgt
    81061 agatggggca actttgtatg ttactacaag accttgtgtt ttgtgtgcaa aaatgtgcat
    81121 aaatgcaggt atattgaaaa ttgtttatga aggtgattat ccagatgata tgtctacaga
    81181 attattaaaa gaagcaggaa tagaactcat aaaattttag tggataaaat ttatatgaca
    81241 ataaatgtca aattatgaca ctgaaataca atggtatcaa atgattaaaa gaaagtattt
    81301 gaaaaatcat cagattagta gtaagatata ttttataatt aaataaattt atgagataga
    81361 ttaattacat gttatttatt ttataataat gagggatact atttatgagt aaaagaaata
    81421 cacatacaga aattgcaaag ttgtttagtt taataagcca aataggattg atgattgtaa
    81481 tatcaatatt aggatgtact ttcttaggaa aatttttaga tagtaaattg aatactactc
    81541 cagttctgac aataatattt ttattgttgg gtgttggagg tgcttttagt ggtgtttata
    81601 aaactctaat agtatataca cgaaggaagt gatatattgg atataaaatt acttaaccag
    81661 gtaaggtcag taacaaaagg tatagtaata tttgatgtag ttttaattgt gctaatgttg
    81721 ataacatcaa cattaagtaa accagtatta ttaggactta tatttggctc tataatagct
    81781 atgttaaatt ttagattatt ggcgatatca ctggaaaaat cagtgacatt tcctgctggg
    81841 aaggcacaag cttatgcatc agcacaatat gctgttagat tatttataat ggcagttgtt
    81901 ctaattgcat cagctacagt accacattta aatataatcg gaacagcatt gggattattg
    81961 agcacaaagt ttgtcatatt atcaaaaaac tttataaata aattaaagag gaaggaggca
    82021 taagtatgaa tcaagcgcat tattatctca taggaaactt taagattagt gaaacaatac
    82081 tagtaagttg gggaataatg gcctttttag cgatagcatc ttattttttg actagaaact
    82141 taaaaaaagt tccaactagt aaagttcaaa tattcttaga atttgcagta ggtggtcttg
    82201 ctaaaatggt tacagatgtt atgggtaaaa atacagtaaa aagaatgcca tatatgatac
    82261 catacatagg aagcttgttt ttatttttcg catgttcaaa tttaagccca ttacttggat
    82321 taaggtcacc aacaaccgac ttagatacaa ctgctgcatg ggcaatgatt acatttttta
    82381 tgatttattt tgcaggagtt aaatttaagg ggttatcata ctttaaagaa ttggctcaac
    82441 cacttgccat aatgttacca atcaatatta taggtgagtt agctagacca atatcattaa
    82501 gtttccgtcc atttgggaat attttaggtg gaacaataat aatgggtctg ttttatcaac
    82561 ttttaggttt tatttctagt ttaataccag gattatcaat accaattgga gaaatattaa
    82621 taccagtccc attacacttg tactttgact tatttgcagg attgttacaa gcatatatat
    82681 ttataatgtt aacaatggta tttatatcaa gtgcaggaga gttagattaa gaaatcaact
    82741 tatattaaga taaaaataaa ataatttaaa aaattaaagt tttaaggagg aaataattat
    82801 ggaaacagca atagtagcgg cagcatcagc aataggagca gggatagcag ttgcaacagg
    82861 tataggtgca ggtataggtc aaggtatagc agcagctaaa gctgcagaag cagtaggaaa
    82921 ccagccagaa gctaaaggtg atataacatc aactttatta ctaggagttg ctatagcaga
    82981 gtcttcagca atatatggtt tggttatatc aataatactt ctattcgtaa atccattctt
    83041 taaatactta ggaatgtaga tttagtcctt aaatattttg agataggttt ataggtaaat
    83101 catctcaaac ttataatcat gagatgaact ttatcatctc gtgattataa gtagttatga
    83161 tttatggaaa ggaggataat tatggaaaaa gcactagtag gtataacgtg ggaatttgtg
    83221 ttccaaattg ttaatacttt tataatattt ttacttctga gaaagctttt attcaagcca
    83281 gtattaaata taattgaatc aagagaaaat gatattaaaa gtgatttggt agaaggcgaa
    83341 aaagcaaaaa atgaaggttt agctctgaaa aaagaatatg agtcaaaaat caattttgcc
    83401 aaagatgaag gtcaagaaat cataaaacaa gcaactataa gagcagagca gaaatcagat
    83461 gatatagtaa atactgcaaa gaaagatgct cttgatataa aagaaaaagc aaacaaagat
    83521 atagaacaag aaagacaaaa ggttataaat gaaatcaaaa atgatatatc taacatagca
    83581 ctacttgcag catctaaagt tattgaaaaa gatttagata aatctaaaca tgaagaattg
    83641 atagaaaact ttataaaaga ggtaggggaa gctaaatgat aaatgtaata gcaaatagat
    83701 atgccgaagc cctatttcaa ttaggagaag aagaaaattc aactgatgtc ttattcaaag
    83761 aactagaaaa agtagtagat atgatgacta aagttagtaa agatttctac aaagttttaa
    83821 aatcaccatt agtttcaaaa tcagaaaaga aaaacttggt agaaattatt tttagtaaag
    83881 aagtaagttc taacataaaa aactttttaa aagttttagt agataaagat agaatatcat
    83941 atttagaaga tatcgaatta gcttataaag aattattaaa taagaaaaac aatgtaattg
    84001 atggagtagc aataagtgct attccaatga gtgaaacaga tattaaagaa cttgaagtca
    84061 aactttcaaa taaatacaac aaaaatgtta ctatagaaaa tgttgttgac aagactatac
    84121 tagggggagt tctagttaga attggaaatg aacaaataga tggcactgtg aaaactcgtc
    84181 ttgataagat gaaagaaaag ctatctgaag taatatctta agaatggcgg tgaacccatg
    84241 aacttaaaac ctgaagaaat aagttctata attaaacagc aaataaaaaa ttatgagaat
    84301 aaagttgagt tgacagatac aggtagtgtt ttaacagttg gggatggtat agcgagtgta
    84361 tatggattag aaaaagctat gtctggtgag ttgctagagt tcccaggtga aatatatggt
    84421 atggcactta acttggaaga agaagtagtt ggagctgtta tccttggaga tgacagtgaa
    84481 ataaaagaag gcgatatagt taagagaact ggaagaatag ttgaagttcc agttggagaa
    84541 gctttaatag gtagagttgt aaactcacta ggtcagccaa tagatggaaa aggacctata
    84601 gcgtacacaa aaactagacc agttgaatct gaggcacctg gtataataga tagaaggtct
    84661 gtttatgaac cattacaaac aggaatcaaa tcaatagatt caatgatacc aattggtaga
    84721 ggtcaaagag agttaataat aggtgacaga caaactggta aaacatctat agttattgac
    84781 actatactaa atcaaaaagg aaaagatgtt atctgtatat atgtagcaat tggacaaaaa
    84841 cgttctacta tagctcaatt agttagctct ttagaaaaag gtggagcttt ggattataca
    84901 atagtagttt cagcaacagc atctgaatca gcaccacttc aatatatagc accatatgct
    84961 ggagcagcta tgggtgaaga atttatgtac aatgggaaac atgtacttat agtgtatgat
    85021 gatttaagta aacaagctgt tgcatataga gaaatgtcat tgttacttag aagaccacca
    85081 ggacgtgagg catatcctgg agatgtattc tatttacatt caagattact tgaaagagca
    85141 gctaagttat ctgatgaatt gggtggaggt tcaatgactg ccttgcctat aatagaaact
    85201 caagctggtg acgtttctgc atatatacca acaaatgtta tatctataac agatggtcaa
    85261 atatacttac aaccagagtt attctattca ggtgtaagac cagcagttga ccctggtata
    85321 tcagtatcaa gggttggtgg ttctgcgcaa attaaagcaa tgaaaaaagt tgcaggaaca
    85381 ttaaaacttg catattcaca atatagagaa cttgcagcat tctcacagtt tggttctgac
    85441 ttagatgaag atactaaaaa gagacttgct caaggtgaaa gaatcgttga gatattaaaa
    85501 caaggtgagc atcaaccaat aaaagttgaa aatcaagtta tgataatata tgctgttata
    85561 aataatcatt tagaggatat tccgatagat aatatagcta gatttgaatc agaattgtat
    85621 gcatttgtag ataataatta tccggaaata tcaagaaaaa tattaggtgg agaagacttc
    85681 actcatgatt taacagatgc aataaatgaa tttaaagaaa agtttgttgt tgaagtataa
    85741 tcctttttaa aaaaggaggt aaattaattg gcaggagctg gaatcaaagc aatcaaaaca
    85801 cgtatgggaa ctgttgaaag tacaaagcaa ataactaaag caatggaatt agttgcatct
    85861 tctaagctta gaaaagcaaa agaaaaggct gaagattcaa gagaatattt taatacttta
    85921 tacaatacag taaaagatat tgcccaaaca acacgtggaa ttagaaatga atttttaaaa
    85981 gaaagagaag taaaaaataa atgctatgta gtagtagctg gagacagagg acttgcaggt
    86041 ggatacaatt ctaatgtatt aaaggaatct gtctctcata tgaaaggtaa aaaggaaaca
    86101 gtgattactg ttggaaagaa agcacatgaa tttttttcta aaagaaacta caatatagca
    86161 aaagatataa atagtgttga agcttgtgga tatgaagatg cgacagaaat ttcacaaact
    86221 attattgacc tgtataaaaa aggtgaagta gatgaagttt atattgctta tacaaaattt
    86281 aaatctgctt tatcacaaga agttgaaatt ttgaaattgc ttccattagt ctttgaaaaa
    86341 gatacagacg aacaaggaat ggcagtaaaa gaagaaaaac aaaatagatc aaaagtacaa
    86401 tatataccat cagcagaagc agttttaagt tatattgtac ctcagtttat atcaggtaca
    86461 gtatatggtg gaattttgga atcttttgca tcagagcaag gtgctagaag aactgcaatg
    86521 gaatcagcca cagacaatgc gaatgaaatg ctatctaatt tagagttaag ttataacaga
    86581 gcaagacagt cagctgtaac tcaagaaata acagagatag ttggtggagt tgaagctcta
    86641 aaatagggag gttaggaaat ggcaaatgta ggtaaagtag tacagattgt aggagctgta
    86701 ctagatgtta agtttgatag tgaacaaagc ctacctaact tattaaatgc gttagtaata
    86761 aagttaggag ataaagaaat agttgcagaa gttgctcaac atataggtga tgatactgta
    86821 agatgtatag ctatgagtgc aacagacgga cttgtaagag gtatggaagt tgtagataca
    86881 ggagggccaa taagtgttcc tgttggagat gaaactttag gtagaatatt caatgttctt
    86941 ggaaaaccag ttgatgggaa accagctcca aaatctgcac ctaaattacc tatacataga
    87001 ccagcaccgg cgtatgatga actagaaact acagcagaga tactagaaac tggtattaag
    87061 gtagttgact tactagctcc atatctaaaa ggtggaaaaa taggtctatt tggaggagca
    87121 ggagtaggaa agacagttct aatacaagag cttataaaca atattgctaa gcaacatggt
    87181 ggtatttctg tattttcagg agtaggagaa agaacaagag aaggtaacga cctttatggc
    87241 gaaatgagtg agtctggagt tataaataaa acagctctag tatttggtca aatgaatgaa
    87301 ccacctggag cgagaatgag agttgcttta actggactta caatggcaga acattttaga
    87361 gatgagcaag gacaagacgt tttacttttc gttgataata tattccgttt cacacaagct
    87421 ggttcagaag tttcagcact tctaggacgt atgccatcag ctgttggtta tcagccaaca
    87481 ttagctactg aaatgggtgc acttcaagag agaataacat caactaagaa aggttcaata
    87541 acatctgttc aagcagtata tgtaccagca gatgacttaa ctgacccagc accagctaca
    87601 acattctctc acttagatgc aaaaacagtt ttatcaagac aaatatcatc actgggtata
    87661 tatccagcag ttgaccctct tgaatctaca tcaaggattc ttgaccctag tatagtagga
    87721 aaagaacatt atgaagtagc tagaggtgta caatcaatac ttcaaagata taaagaactt
    87781 caagatataa ttgctatact tggtatggat gaattatctg atgaagataa gttaatagtt
    87841 gcacgtgcta gaaagataca aagattctta tctcaatcat tcacagttgc tgagcaattt
    87901 acaggaaatc caggtcaata tgttcctgtt aaagagactg taagaggatt taaagaaatt
    87961 cttgaaggga aacatgatga cttaccagag tcagcattct tatttgtagg aacaatagaa
    88021 gacgctgtta gaaaagcgaa gggaagtatg tagtcatggc cagtgaattt tcattgaaag
    88081 ttgtaactcc aagtaatact ttttatgatg gagatgttga aatgatagta gttagaacta
    88141 caaaaggaga caaaggggtg ttgaaaggtc atataccatt tgtttcaggt atacctgtag
    88201 gaactttaaa gataaaaaaa gagggaagtt ttaaagaggc gaaaatagca gaaggattca
    88261 ttaatgtaac tcaagataaa actgtcatat tgacagaatc tgctgagtgg atttaataaa
    88321 ttaatttaga gtataaaaag tcattttgac caaaaaaact tttgggtaaa atggcttttt
    88381 tgtgttttgg tgttaataaa ataacgataa aattgtaagt ttttagtgat aataaattca
    88441 caaaatgttt aaatttactg ccaaattatg ttgtaattaa taagataaag aagatttaga
    88501 tagcttttat gtaagtgaaa aagtcttcat ataagaaata catataaaat ttttataaaa
    88561 tttaaaaaat ttgcttagtt gtgtagtata ataaataata taatagattt gtatataata
    88621 ttcattacaa ataattttaa aagttacagt tttattataa tgattattaa attaagataa
    88681 gtaacataaa ctaaataata atgtgtatta tcgatagtaa atttataaat tcattattaa
    88741 ttattgaaag aagtattata aattttctgg aggaacaatg aatatatttg atattggcgt
    88801 agatataatt gaaattgata gaataagaaa ggctgtagac aaaaataata ggtttttgga
    88861 gaagatattt acagatagag aaatagagta ttttaattct aagaatttta aagcagaaag
    88921 tatagcaggt aatttcgcag ccaaggaagc tataagtaaa tctataggta caggaattag
    88981 agtgtttaat tttaaagata tagaagttct cagagatgaa atgggaaaac caatagtaaa
    89041 aacttataat aatctagcaa aaatgtgcat agattataat gttttagaaa taaaagtttc
    89101 aatatcacat tcaaaagatt atgctatagc aaatgcgata accataatta aggactaagg
    89161 agtgattaca ttatgatgga taaaacagca aaagaaataa tgacaacaga tgtaatagta
    89221 gcaaaacaag atgatagtat agcagatgta gctaatatgc taattgcaga aaaaatagga
    89281 ggtctgccag ttgtagattc agagaataga gttgttggaa taatttctga aacagatatt
    89341 cttaaaaaag aaaaatatat agaagcacct ctctatataa atctattaca aggattgatt
    89401 ttcttagatg acttaaaaaa agttgaaaaa gatataaaac aagtagctgc ttataaggtt
    89461 ggagaactta tgtctaaaga tataataaag gttcatgaag atgataagtt tgatgatgta
    89521 gctaatgtaa tgattaaaaa atcaataaac agagttcctg tagtagatga tgacaacaaa
    89581 cttaaaggta taatttgtag atatgatata ataaaagctt tatacaatga ataaactgaa
    89641 gtaggaggtg tgatgtgaga aaaaagtgga ccatagtatg tattatgttc ttggcactgg
    89701 ttataattgt aataggatgc caaaaacgac agtccacaaa agaagaagta tacaaagatt
    89761 ttcaaaagca gatttctgat atgaattatt acagttgtaa agctgaagta gaagtagttg
    89821 gaaataaaag tccacacaat tatgttttaa ttcatactta taaaaaaacc gataactata
    89881 aattagaagt aatttcccca aaacatttaa aagggaaaag tatagaatat caaggtgata
    89941 aaatattagt taaaaatcca aagattagtg atgtagtgga gttacctaat acaggaaaaa
    90001 ataatcaata tctttttgta ggagacttta taaaaaacta tcttcaaaat gaggagatga
    90061 aagtaaagtt atccaaagga catttggttt tagagacttt tattcctggg gataataaat
    90121 attttaacaa gcaagtatta tatgtaaatg ctgacacaaa aaatcctgaa aaaatggaag
    90181 tgttagataa agagggagtg ccaagattta cagtaaaata caaagatttt gaatacagaa
    90241 actaaggggg actaaagaca tgcaaaaaat aacagtgcct acatgggcag agataaatct
    90301 agataactta agatttaact taaataatat taaaaattta ttagaagaag atattaagat
    90361 ttgtggagta ataaaagctg atgcatatgg acatggtgca gtagaagttg caaaattgct
    90421 agaaaaagaa aaagtagatt acttagcagt agcaagaact gctgaaggaa ttgaacttag
    90481 acaaaatggc ataacacttc ctattttgaa cttgggatat actccagacg aagcttttga
    90541 agattctata aaaaataaaa taactatgac agtttattct ttagaaacag cacaaaagat
    90601 aaatgaaatt gcaaaatctt taggagaaaa agcctgtgtt catgttaaaa tagactcagg
    90661 gatgactaga ataggtttcc aacctaatga ggagtcagta caggaaataa tagaattaaa
    90721 taaattagaa tatatagatt tagaaggtat gtttactcat tttgctacag ctgatgaagt
    90781 aagtaaagag tacacttata aacaagctaa taattataaa tttatgtctg ataaattaga
    90841 tgaggctggt gtaaaaatag ctataaaaca tgtatcaaac agtgcagcta ttatggattg
    90901 ccctgattta agattaaata tggtaagagc aggaataata ttatatggtc attatccatc
    90961 tgatgatgta tttaaagata gattagaatt aagaccagcc atgaaattaa aatcaaaaat
    91021 cggacatata aaacaagttg aaccaggtgt aggaataagt tatggactaa aatacacaac
    91081 tacaggtaaa gaaacaatag ctacagttcc aataggatac gcagatggat ttactagaat
    91141 ccaaaaaaat ccaaaggttc ttattaaggg agaagtgttt gatgtagttg gtagaatatg
    91201 tatggatcaa ataatggtta gaattgacaa agatatagac ataaaagttg gagatgaggt
    91261 tatactattt ggagaaggcg aagttacagc tgagcgtata gctaaagact taggaactat
    91321 aaactatgaa gtgttatgta tgatatcaag aagagttgac cgtgtttata tggaaaataa
    91381 tgagcttgta caaataaaca gttatttgct aaaataaaac catgatatta taatctggga
    91441 ggcgattttg tgtacgataa gagtaaagaa aaaatagaag ttactttacc tgactctctg
    91501 atttctgaag ttgatagtat agttcagatg gagaacagta acagagaaga ttttgcaaaa
    91561 gctgcttttc agttctatat aactcagaaa aagaaaattc atattaaaga atctatgaaa
    91621 acgggttata gagaaatggg tcaaattaat ctgtcactag ctgaattagg tatgacagtt
    91681 gaagaagtat cttctgatga taaaaatgaa gggaagattg cccaaggtga gtactaattg
    91741 tgaaataaag cgtggtgatt tatattacgc tgatttaagt ccagttgttg gttcagagca
    91801 gggaggcgtt agacctgttc tgataataca aaatgatgta ggtaataaat atagtcctac
    91861 agttatagta tcagctataa catctcagat taataaagcc aagttaccta ctcatgtaga
    91921 gataagttcc aatgaatatg ggcttaataa agactcagta attcttttag agcaaattag
    91981 aactatcgat aaaaagagac tgagggaaaa aattggttgt ctagatgaaa atatgatgat
    92041 aaaagtagat gatggacttc aaataagttt aggattgttt actttttaaa gagggatttg
    92101 tcttttatta gacaatccct tttttctttt aaatatctct taaggtttaa ttttaatgta
    92161 ctatgttaat aaaattaaaa ataacgcaaa aaaatagcat aaaagtgata taataatatg
    92221 gaattgatat aaaattacga actcaaggga ggtaattgca tgagagacca taaaggatta
    92281 aatgagattg cgcgtatcat aagaagagat atagtttcaa tgatacatag agccaaatct
    92341 ggacatccag gagggtcatt atcagtagtt gaaatactta ctgcgttata ctttgatgaa
    92401 atgaatgtgg attcttcaaa tcctaaaatg gaggatagag atagatttgt attatcaaaa
    92461 ggacatgctg caccagcatt atatgctaca ttggctgaaa aaggttattt tgataaggaa
    92521 gaactgaatg gattaaggaa gataggaagg atgttacaag gtcatccaga catgaaaggc
    92581 acgccaggtg tggaaatatc tactggttct ttaggtcaag gtttttcagt tgcatgtggt
    92641 atggctatgg catcaaagtt ggataatgct ccgtggaatg tgtatacttt gcttggtgat
    92701 ggtgaggttc aagaaggaat tgtttgggaa gctgctatga gtgctgcaca ttataaactt
    92761 gataatttaa ttgctttttt agataataat ggacttcaaa tagatggaga tatagaaagc
    92821 gtaatgagtt taggttcaat agttgataaa tttaaagctt ttggttggaa tgtaattgag
    92881 atagatggac atgattttga ccaaatattt gcggctcttg atatagctaa atcaacagtt
    92941 aaaaaaccta caatgatagt tgcaaaaact ataaaaggca aaggaatatc ttttatggaa
    93001 aatcaagcag gatggcatgg gacagctcca agtgatgagg agctagaaaa agccttgctt
    93061 gaattaggag gtgctgacaa tgagtaaaat ggcgacgaga gaagcatatg gtaaagcatt
    93121 agtaaaattg ggtcaaataa atgacaatgt tgtagtatta gatgcagatt tatcaaagtc
    93181 tacaaaaaca catgattttt acaagtcatt tccagataga ttttttaata tgggtatagc
    93241 tgaacaaaat ttaataggcg ctgcttgtgg attatcaact gctggtaaaa taccttttgc
    93301 aagcactttt gctatgtttg ctacgggaag ggcttttgag attataagaa attcagtttg
    93361 ttatccaaag ctaaatgtaa aaatatgtgc tactcatgca ggtcttactg ttggagagga
    93421 tggagcatct catgaaagtg tggaagatat tgctataatg agagctatac caaatatgac
    93481 agttcttgtt ccagctgatg gagttgaaac agaaaaaatt atttttgaaa tagctaagta
    93541 taatggacct gtttatgtta gattagggag aagttcagta ccagttttat ttgatgaaga
    93601 ttataaattt gaaataggaa aaggtacagt cttaagagaa ggaaaggatg tatcaattat
    93661 agcttgtgga attatggtaa atgaagctct attagcacaa gaaaagttgc aagaagaagg
    93721 tataagtgct agagttataa acatgtcctc gataaaacct atagacaaag atttaatatt
    93781 agaatcagca aaagaaacga atgctattgt aactgtagaa gaacatagca taattggggg
    93841 attaggttct gctgtaagtg aggtagtagg agagtcttgt ccaaccattg taaagaaagt
    93901 tggaattaaa gatacatttg gagaatctgg tactccaaat gaattgttaa aaaaatatga
    93961 attaacttgt gatgacataa taaagactgt taaagaagct attattgcaa aaagaatgta
    94021 agagatataa attattcatg tttgttaata atataatata tttaaaagag aggtataaag
    94081 tgttatatta atttatatct ctcttttttt tatatgatat caatatatca aaaatgtaat
    94141 aaaatattca aatttttaca aaaatagaga aaatattcaa atatatattg actattcaga
    94201 aaaatatgac taatatataa tttatagaga aactaatttt atatataatg actacttaga
    94261 taaaagaatc taaaaaaatt atgagaggag gagtagggta taaaatcaga ttataagttt
    94321 taagttatta tactaaaaag ctataaaaca gttatcatgt attttatgtt aaaggaaatt
    94381 gtaggtagtt tatcgaattg tttaatcaga agaaaaaatt tcacatgggg aggtaactga
    94441 gatatgtcag aaaaagtaaa tgttaaaaca gtcatcagtt tcgcaggtgc gtatgtagca
    94501 acagtaatcg gttctggatt tgcaacagga caggaaataa tgcaattctt ctctttttat
    94561 ggttttgcag gtattattgg tgggataatt tcaatggtat tattctcatg gatgggtgct
    94621 tcagttatgt caaaaggtaa agagcttcaa cttaaagaac ctatcaaaat ttatcgtgtt
    94681 tattgtggta agtatttagg aatattcttt gaatggtttg gacctttatt cttatttggt
    94741 gttttcgtag taatgatttc aggagcagga gcaactttaa ctgaatacta tggattaaat
    94801 ccatttgtag gtagagtagg tatggcagta gtagcattat taactgtttc actaggtctt
    94861 gataaattat caaagatact aggtggaata gggcctataa taataatctt tacactttta
    94921 gttggtggta taagtttagc taaaaacata ggtaatatag gagaagctgc acaagttctt
    94981 agcactgtaa agatagctaa accagttcca aatgcctatc tatctggtgt aatatatact
    95041 acttataaca caattgttgt tatggcattc ttaactggat taggagcaag tgctgctaac
    95101 aaaaaagaag ctatttgtgg tggtatatta ggtggagtag cacttatggc tgcagctata
    95161 atgatgcact tagctattct ttctgatata ggaaacttat attcaaaagc aataccatca
    95221 ttattcttag cagataagat ttcaccagta attggagttt tattctcaat aattcttata
    95281 ttaggtatat atacaacagc tgtaccttta ttatggtcag taactaatag atttgtagat
    95341 gacaaacatc ctaagtttaa attaataact ttaatagcag ctatattagg tcttataggt
    95401 ggcttcctac catttgataa gctggttggt attttatatc catatactgg atatatgggc
    95461 gtcataatac tggtttgtgt tctttataga cagattacaa aaacatctgg atatcaagaa
    95521 ggaatcagtg acaaataagt attttttaat attgtaaact caagagctat atgaaaacaa
    95581 tgaaaatgtt ttcatatagc tctttttatt ttttagctcg tttattatga aatagaaaaa
    95641 acatgttttt tagttacatt acatatattt ataattatgt ataaatatat actaatcaat
    95701 aatttagagt ctaagataag tttataaatc atatatttag aatatatggg aggagtgata
    95761 agattgaatg taaagtttaa tattaaaggt ataatttatg gagttatagc tcttgtattc
    95821 gttatagggg gatttatatt cataccaagt atgtttgtag agaaatctaa acccatagat
    95881 tatacagtat tacaaagaaa ttcaatacca gaaaagatat tagatgtgat ggataagtat
    95941 acaaatgaag aaagagcatt agcggctaaa atcgataaca aaatatacat tatagtgact
    96001 agaggaaata ataaatatgg tatagaaatg gacaaaatag aaagtgtggt tgaggaagga
    96061 aaggatgttt tgagagtcaa gataaagtat aaagataagg aagattcttt tccatacata
    96121 gttgttgaaa caaatatgag tgaacttcca gatagaatag aattaaatgc gacgaaataa
    96181 gaaggtattt tataattaaa taactgcata ttgtacaata aagtcatggt ttttattaat
    96241 gtaagtaaat tgtgtatggt tcttattgtt gtaaaagcca tgacttaaaa tgcttataaa
    96301 gtaagatatt aacagtagta tttattacta cattaaatgg ggtctggaaa tacttaatat
    96361 tctaactatt tggagtgata attctagttt cagtaactat gtagtctaat tgtgcatcat
    96421 gagtttcttt tggaatagaa tcgaatatct ggaagtcaaa tgctatgccg atagttatag
    96481 catcttttct taagttttct aaaaatctat cataaaatcc tccaccatat cctaatctat
    96541 agcaatgtat atcatatgct acagctggta caattacaac atctaaggcc ttaatatcaa
    96601 ttgtaggtga tttttcgtta ggttctctta ttccatatgc tccaattctc aatccctctt
    96661 ttaaatctgt tatttgagta gggattaatg tatgattttc ttttattgtg atgggacttg
    96721 atactatttt ctttagagac aaaagttttt tgattaaatt atctgtttga acttcattgt
    96781 taaaatttag ataaagcatt atattagttg catttcttat atcttccatt tgaagtaatt
    96841 tttcagttat taaatttgaa ttactaagta caaagtttgc attcttattt tttcttgatt
    96901 caataacttt ttttctaaat tcttttttca taaaataatt cctcccttaa tatgttataa
    96961 tgtgtacagc aattgtattt aatataaatt ttagtataat ttattgtaat aaagcaatat
    97021 atttgggtaa atgtaataaa aataacagct agtcatataa taataatagt ttatgtaaaa
    97081 ggagatgtat cttgtgattt caaagaagaa agctattttt ttaggggtga ttttagtaat
    97141 tataacagct atggttacat cagcatttca gttgacattg gggaataaag ttgtaatatc
    97201 gaaagaactc tatgaagact ataaaaaata tgataagttg ttaggactag aatctattat
    97261 aaaacaagat ttttataaaa aagtatcaga tactgatttg gtaaatggag cagcaaaagg
    97321 tctatttcta ggaacaaatg ataagtattc tggttattat actaaagatg aaatggagaa
    97381 tctaattaat gatagtgaag gttcttatgt tggtgtaggt atgtatatag gggcatcaaa
    97441 agatggtggc ttagtagtag ttcccatgaa agattctcca gcagagaaag ccggtgtaaa
    97501 atcaggggat aaattagtaa aagtcaatgg aaaatctgtc tcatataaaa attcagatga
    97561 agctgttcgt atgatgaagg gtaaaaaagg taaaacagtt gaactgacta tattaagaga
    97621 agataaacaa ttgaacttta aagtaaaaac agaccagata attgagaaga gtatagaaag
    97681 taaagtaatt gataatgatt taggatatat agaaattacc caatttatat caagtacata
    97741 tactgacttt gataaagctt taaaagaatt aaaagcaaaa aatatcaagg gattagtaat
    97801 agaccttaga aataatcctg gtggaatgct agatatatgt aaagaagttg ctgatgagtt
    97861 aattggtgaa ggaactatag tatatacaaa agataataag ggaaatactg agtacttgaa
    97921 atctgataaa gaaaaattgg gacttccaat agtagtgtta actaatggag aaagtgcttc
    97981 tgctgctgaa atattaacag ctgcaatcgt tgataataaa gaaggtattt ctgtagggac
    98041 tacaactttt ggtaaagggt tggtacaatc agttgtaaga ttgaaggatg gaacaggata
    98101 caagctaact actgctcaat attttacacc taatggggat tatatcaatg aaaaaggtat
    98161 aaagcctact attgaagaga aagatgaaaa taaacagtta gatgtagcaa ctaagtggct
    98221 tagagaacag attgataaat aggatttaat ttttataaac ttatactata aaataaagaa
    98281 cattataaaa gagatgtctc aaaatgaact ttttagttca tgagggagtc tggaaatatt
    98341 tatgtgtatt ctttaaataa aaatttattt tttaagtgat aattaaattt ttacagacag
    98401 ggctatggtt ttaaaaatca tagccttatt ttgatatttt ttaaaaacat attgcaaccc
    98461 agttgttcca aatattatat catttacttt taaaatatcg caaactgcat attatatatt
    98521 cattataata taatttatct aactagaacc tcagtttact aattaaatta gatgca
//
LOCUS       DAEPIN010000006        97925 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.006, whole genome shotgun sequence.
ACCESSION   DAEPIN010000006 DAEPIN010000000
VERSION     DAEPIN010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 97925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 97925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..97925
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.006"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            complement(93..353)
                     /locus_tag="KR250_000520"
     CDS             complement(93..353)
                     /locus_tag="KR250_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich small domain-containing protein"
                     /protein_id="HBH0066489.1"
                     /translation="MGENYKFFNHKDCEFFPCHKTNKPEEFNCLFCYCPLYALGENCG
                     GNFKYTDKGIKDCSSCILPHKKDNYNYIMSKFQDLVKITSKK"
     gene            570..1853
                     /gene="hflX"
                     /locus_tag="KR250_000521"
     CDS             570..1853
                     /gene="hflX"
                     /locus_tag="KR250_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HBH0066490.1"
                     /translation="MKEIQEKALLVGLNLTTIVKKNDDIDTNESMEELKELTKAAGAE
                     VVGSLIQNKHSVDAAYYIGKGKVEEIRAYSDSLDATLVIFNDELSGAQIRNIENVVGR
                     KVIDRTTLILDIFAQRALSKEGKLQVELAQLKYRLPRLYGMGGEMSRTGAGIGTRGPG
                     EQKLEIDKRHILNKAADIRRELKEVKKNRETQRVKRLKSNIPIVALVGYTNAGKSTLL
                     NELIKTHKDYEQEKEVFVKDMLFATLDVTLRKALLPNKKEFLVVDTVGFVSKLPHDLV
                     EAFKATLEEVQYADLILHVIDATNTSYELQKSTTEGVLKELGVNDKKHILVYNKVDKL
                     ELDIYPKSQEDIVYISAKQGINMDKLLNMIEIALMENTYSVSLMLPYERGDIFSRIKD
                     KYNVENFEYGESGITLDVDLDEEDFNIYREYILEK"
     gene            1861..2538
                     /locus_tag="KR250_000522"
     CDS             1861..2538
                     /locus_tag="KR250_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HBH0066491.1"
                     /translation="MDINWENIDNLEDYFITYLLYKESKTVSQISKIRNISSTEASEQ
                     LIQAKLKIKEMQKDDFEASKDILDKFLELDKIKRLDFMDSLDDEKMVYFKRKVFKRIL
                     VEKNAEDLIVLIWATGELKDDRFLKLLHQLTNHRHSDIRRITYSAIRKIESPSSREVL
                     QKGLYDKNAQTRQYCAKALSKLGDENSLKILQQLKDKNKNFEKEYVLRAYDKAIKSLE
                     NAKNLKK"
     gene            2624..3067
                     /locus_tag="KR250_000523"
     CDS             2624..3067
                     /locus_tag="KR250_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBH0066492.1"
                     /translation="MIVRRCAGGVVFYANKVLIVKNDRGEWTLPKGKILGGGLPYESA
                     VQRVKVETGIDAKMIDVAGDTMYEFFSRSRQQEVCNAIMWYVMEACNTDCVLAPEFQE
                     GGFYKVKDALEMLSHHKEQALVEVSYKKFKELKKLVSEGDDVPAN"
     gene            3372..4016
                     /locus_tag="KR250_000524"
     CDS             3372..4016
                     /locus_tag="KR250_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HBH0066493.1"
                     /translation="MSNYRTLHEFGMDEITIEKSVFIGYAKPIKSEEEALEFINEIKK
                     KHKDATHNVWAYTVGKNMNIQRYSDDGEPQGTAGIPTLEVIKKEDLRDVAVVVTRYFG
                     GTKLGAGGLVRAYTKGAKLGLEAGKIIYKVMYQEVKVKIDYTQLGKVQNELMNLGYFI
                     KDTVYEDNVEIVVYSRLEDVEKLSEKMIDITSGTGKIVLGEEFYLSEQNGEILI"
     gene            complement(4102..4479)
                     /locus_tag="KR250_000525"
     CDS             complement(4102..4479)
                     /locus_tag="KR250_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HBH0066494.1"
                     /translation="MLFALIGFFAGIIGGMGMGGGTILIPALVLLAGIDTKIAQSVNL
                     LSSIPMTLIALAIHIKNKNVIFKLVIPIAVFGVLGAVFGSLLANYLSSDILKKIFGIF
                     LLLVGLFEVKKGFCAKSDKKPLN"
     gene            complement(4479..4865)
                     /locus_tag="KR250_000526"
     CDS             complement(4479..4865)
                     /locus_tag="KR250_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HBH0066495.1"
                     /translation="MKKTDDLKKKYFDFNLKNTIIGVFTGFINGVFGSGGGTLLVPIL
                     NDIVKVEEHKSHATALSIIIFLTTASSVLYVSKGTYDVNLTIKVAVGSILGGIIGAKL
                     LNKVTGKFLRISFGIIMIIAALRMVF"
     gene            5044..5799
                     /gene="nadE"
                     /locus_tag="KR250_000527"
     CDS             5044..5799
                     /gene="nadE"
                     /locus_tag="KR250_000527"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HBH0066496.1"
                     /translation="MSNIKIQIDKTVEWLINKVNEANAKGLIVGVSGGIDSAVVANLI
                     KKAFPENSMGVIMSIKSNPQDREDALKVIEGCDIEYLDLDLIEPQSAILDMVVGNLKD
                     KHLYREEYLKMTDANLRARVRMSTIYTIANNLGYLVVGTDNAAEIHTGYFTKFGDGGV
                     DILPIANLTKGEVYEWAKELGVHEDLINKAPSAGLWEGQTDEDEMGTTYNMIDAVLEG
                     RLDEVPKRDQEIIERLHRLSEHKRKTPAQPPKF"
     gene            5882..6601
                     /locus_tag="KR250_000528"
     CDS             5882..6601
                     /locus_tag="KR250_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBH0066497.1"
                     /translation="MGRIGNIINRKGKQDAQRAKIFTKHARAIAVAAKEGGADPEYNA
                     ALKTAIEKAKADNMPNDNIDRAIAKGAGAGAGEDYETIVYEGYGPGGVAVIVETLTDN
                     KNRTAGNVRYYFDKNGGNLGTSGCVSFMFDKKGQILVGLGDGVSEEELMDVALEAGAE
                     DFITEEDGYEIITTPEDFSSVRDELKAKGYEFISADVKMIPQTTTVLTEESHLKMMNK
                     LVDMLEEDDDVQDIYHNWEVE"
     gene            complement(6768..7772)
                     /locus_tag="KR250_000529"
     CDS             complement(6768..7772)
                     /locus_tag="KR250_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBH0066498.1"
                     /translation="MATIKDIAKDCGLSSATVSYVLSGKGNERRIPLHTQELILNSAD
                     KLGYKRSTPIKHNNIKIAFYWQDEGFELASTNILQAINHIISLEHLNINIIIRPYKVG
                     HLCDDEYFFANNYYDGIVLTGINADDIEYLVKNKPKIPVVVVNREIPGFSSVSIDNEE
                     TGRIAALHAIQNGKNDIAVVFNPTSFTCMTLRGKSFLKTCKEHGIHLSDSIYYCSNKI
                     DSGYDIAQKMLAKNSLKKVIFCNHDVVALGMMNAFHESGIVIGEDIQIFAASNGPSRL
                     MARSIPPLTVIDLKMEETAERSLRMIIDTVTGRINGIQQQVIYPSIIYRQSSPAQNLL
                     "
     gene            8038..8934
                     /locus_tag="KR250_000530"
     CDS             8038..8934
                     /locus_tag="KR250_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HBH0066499.1"
                     /translation="MSKVNIFEVGPRDGFQNLKEFLETDKKLKIIDGLIASGIKQLEI
                     SSFVSPKAIPQMKDAKEVATYCVEKYPDVKLYALVPNLYGAKVAWECGIRDISYVISL
                     SETHNKKNINRTHEESIDELKKIMNTYPDMNICIGMATVFGCPFEGLPKIEDAIRFAK
                     RLWENGIREMNLADTIGIADPKQVRETISALVEALPECRWQVHIHDTRNMGMVNTLVA
                     IESGITTVQSTLGGLGGCPFAPGASGNTATEDLVYMLERMGYDTGVDFVKLLELSKYQ
                     KSFVNGVYSGHHVNIKSDSCNC"
     gene            9072..10334
                     /locus_tag="KR250_000531"
     CDS             9072..10334
                     /locus_tag="KR250_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0066500.1"
                     /translation="MDIIALLLFLGVIIIAFVRKNNAGILALAIGVIAVRIFGMDDKE
                     LYGAISNSMFTTLVGITLLFAIVNSTGALKLVAEKIVAASGKRVWLIPIAVFIAGFVV
                     AGVGPGAIPALAIIPAMAVPVAITVGYNPVMLALIGECGLMAGRMTPITPEGQIITTV
                     AEGVGIENVMPTILACQTLSTIVFSIVLFIIFKGYKLKKPINVIDSKQIEKFNIKQII
                     SLISIVVMIAFIIIFDMNVGLAAFAVSAVLIFFNIADEGECIKSIPWSTIVMVLGVGA
                     MMTIVDAAGGIDLMSNALSSLMNSKTATPIMSISAGLMSMVSSALAVVYPTMMPMCVD
                     IAKAVGGVDPLALIAAVGVGGSLAGVSPLSTGGALILAAMGSSKKDFNKEEQNKVFVQ
                     LFIMSAVGLLVIAVVSILSFNAITHLMN"
     gene            10410..11603
                     /locus_tag="KR250_000532"
     CDS             10410..11603
                     /locus_tag="KR250_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HBH0066501.1"
                     /translation="MKKGALSNIKVLDLTRVLAGPYCTMMLADFGADVIKIEMPRKGD
                     DTRGFGPFVNGESLYYANINRNKKGITLNLKSEEGKNIFLEMVKHADVVVENYRPGVM
                     EKLGLGYDELAKVNNQIIYGAVSGFGCYGPYSERPGYDIIAQAMGGLMSITGQEGGEP
                     TRVGNAMGDVLGGMNLTIGILTALNARKIIGKGQRVDVSLVDSVVSSLETGIQRYFDS
                     GQPELMGNRYASAYPYDSFKASDGSFVIGCGNDKLFEKLCKKVLFREDLLIDERFKTN
                     LNRCEHYKALKPEIEKWSIKHTIKEAVDLISEAGVPAAPIYNLDCVTTDEHIAKAREM
                     IVDISHPIIGNMKVNGNPVKLMTTKAKIDMPAPSLGESNEEIYNGWLGFDSDKIKDLK
                     ENGVI"
     gene            12232..13026
                     /locus_tag="KR250_000533"
     CDS             12232..13026
                     /locus_tag="KR250_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HBH0066502.1"
                     /translation="MESVMENLNNLKVEVKDKVCVITINRPKALNALNSDTLRELSQV
                     IDVVSENEAILGVIITGEGKVFVAGADIRQMQNYKSEEGRKYAGYAQGVFDKIEALEK
                     TVIAAVNGYALGGGCELAMSCDIRIASEKAIFGQPEVNLGVIPCFGGTQRLSRLVGTG
                     IAKELIFTGRQVNAEEAKSIGLINKVVPSDLLLEESMKMMNQIVEKAPIAIRYAKVVI
                     NKGIDMDLKNALELEKDIAGLTFATRDKQEGMNAFIEKRKPVFENK"
     gene            13121..14461
                     /locus_tag="KR250_000534"
     CDS             13121..14461
                     /locus_tag="KR250_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HBH0066503.1"
                     /translation="MNTGVIISLVGIIIALSLLVVMVMKGINIFIIAIVCSSLVAITG
                     SLNVYDALKVDYMTGFVGFLQANFFIFLTGTLMGKIMEITGGAKSIAKMIVRWIGKDK
                     ALLSIPIACGILAYGGVSVFVVSFAVFPIALEIFKEADLPRRFIPAALTFGCSTFAMV
                     APGAPQIQNIVPASTLGTDIMAGAVNGFISCAVMFIVGSTILYKMVSKEKANGGHFIA
                     HESDIFDEAEATNSKRENGPNGVIALIPLIVSILIINVKVNGKAIVPIEVGVFIGALL
                     VYLLLNKYQDNTKIVGHVGDACKTTVVAICNTCAVVAFGSVVKSAVGFDFIVNAMTNI
                     PGPPIAGAAVGTTVIAGICGSASGGLGIAAPLLGPVFLAKGVSAAALTRTMAISSAAL
                     DSLPHNGYIVTVTNGLCKETHKDAYMPIFWVTVLTPFIGTIVAVLLFTMFPGLP"
     gene            14598..15830
                     /locus_tag="KR250_000535"
     CDS             14598..15830
                     /locus_tag="KR250_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HBH0066504.1"
                     /translation="MLNKPLEGVKVIDLTYFVAGPGTSKILADWGADVIKVEPSFGDP
                     GRKTGATMTMPIDDYNNPFYSTYNSNKRGLSINLKSETGIEIMDKLLSEANVFVSSYR
                     TGALKRLGLDYESLSKKHPHLIWGQINGFGDFGPAKDNAGFDTVAFWARSGAMLDIAE
                     KDTSPINPPIAFGDATTSCSLSGGICAALYQQAKTGKGQKVMASLFGQAIWNASSLLA
                     STQFSDEYPKTRKNAISPVINSYRCKDGKWIFLSILEHERYFEALCRLFGREDLIKEE
                     KFASSVESKKNATELINILDGEFAKFTQNEMVEKLTAADIAHEKIQHVNDVVTDEQAI
                     ANNYIYEHTSKDGNKTMLASTPVKFGNIEVNMTCDAPLIGEHSDEILRELGYSDSDIE
                     ALIESGIVTMNKEACEIC"
     gene            15870..17012
                     /locus_tag="KR250_000536"
     CDS             15870..17012
                     /locus_tag="KR250_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HBH0066505.1"
                     /translation="MDFRLTEAQLMLQRVAKEFAENEIAPIAAETDKTGIFPRELFSK
                     MAKIGFNGIGTPVEYSGSGGADIEKVIVVTEIAKKCAASAAILSIHTIYAQAILKFGT
                     EEQKKKYLPMMAEGGCVGAFALTEPNAGSDAARAATTAIIDEETDEYVLNGTKCFISG
                     GGQAESLIIFALTDPSKGIKGMSAIIVDKGTPGFSIGKIEEKMGIHGSETAELIFDNC
                     RVPKSNLLGKEGKGFNIAMTCLDGARIGVGAQAVGIAEGALEESIKYSKERVQFGKPI
                     SALQGIQWYIADMATMVESAKLLVYYAADLKARGEKHTKEAAMAKYNASRTAREVTNL
                     ALQIHGGYGYMKDYPLERMYRDAKITEIYEGTSEIHKVVISRAVLG"
     gene            17023..17817
                     /locus_tag="KR250_000537"
     CDS             17023..17817
                     /locus_tag="KR250_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HBH0066506.1"
                     /translation="MNIVVCLKQVPDTNEVKINKETGTLIRDGVPSIINPDDRNALEE
                     ALKMKDELGAVIKVISMGPPQAKSALKEALAMGADEAYLISDRAFGGSDTWATSTIIA
                     AAIEKVGKYDVIFCGRQAIDGDTAQVGPEVAEFLGIPQVTYAKEVKVQDDKLLVTRYT
                     ETGDYLIEAKMPVLLTAIKELNNPRYPSVKGILEAYNNGDAKITVLTLADLDVDTTQI
                     GLKGSPTNVYKSFVPVKDKHNEIIEGINKKEKAEKLIEILFDLKLV"
     gene            17828..18850
                     /locus_tag="KR250_000538"
     CDS             17828..18850
                     /locus_tag="KR250_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HBH0066507.1"
                     /translation="MMRAKVNQGINLNDYNGVWVIGEQREGKINPVTIELIGEGRKLA
                     DQLGKELAVVIAGYEVEKEVKELLHYSVDKVYYINDPLLKDFTTDGYAISIANLIERK
                     KPEVVLVGATSIGRDIAPRIAGKVGTGLTADCTKLEIDSTDNKLLQTRPAFGGNLMAT
                     IVCPKNRPQMSTVRPGVMAKAVRNESETGILEVVTPELTEKMIRTRLVEILPQEKKSV
                     NLTDARIIVSGGRGLKRAEGFELIKELADKLGAEIGASRAAVDSGWIEHSHQVGQTGT
                     TVRPELYIACGISGAIQHLAGMSDSKYIVAINKDAKAPIFSICDYGIVGDLYEIIPEM
                     IESLNR"
     gene            18949..20325
                     /locus_tag="KR250_000539"
     CDS             18949..20325
                     /locus_tag="KR250_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HBH0066508.1"
                     /translation="MKEQWYKDIFARVLSMTDDGFIVVNRSGVIIDINDKYCDFLGKE
                     RKDIIGQNIQSIIPNTKMLDVMKNKYCEEGAIHHYSGGNTKEKSVIVSRSYVENDNGE
                     VVAGVAQVKFRLQSFDVAKKLMSEYMELQYYKEQFKDNCGFDKLIGENRDFIELKKTG
                     VKASKTNFPVLLTGETGTGKEVFARAIHNNSSRSDKPMVSINCAAIPEELLESELFGY
                     DEGAFTGAKKGGKKGKFLVANNGTIFLDEIGDMPLTMQAKLLRVLQESEIEPVGGLKT
                     IKIDVRVISATRKNLSKMVEEGLFREDLYYRLNVINIHMMELKDRQDDILLLANYILN
                     KLNVEYKELKVLSDKVKNCFINYTWPGNIRELQNVIKSAYAVSDDMVIMMCDLPSKMD
                     NISRVAQCNVDSNCSIHEMVENYEKSLIIDVLRKYNWKCSKAAEVMGIHKSLLYKKIK
                     KYEIELNN"
     gene            complement(20657..21001)
                     /locus_tag="KR250_000540"
     CDS             complement(20657..21001)
                     /locus_tag="KR250_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0066509.1"
                     /translation="MDQIKRLHELQQKSYLTKEEFEELKNNTYIERFELRDECDNSYI
                     YTFYTNDKCNDIKFIKSQFIVTLIKIGFLCECGGVFRQKKIVDEYDYGYNAIYKCDSC
                     GKELDKEVENYD"
     gene            21489..22499
                     /locus_tag="KR250_000541"
     CDS             21489..22499
                     /locus_tag="KR250_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HBH0066510.1"
                     /translation="MHEDKNDIFESIDTEDFDNKTEEECLELVRKNGLNLIYIKNQTE
                     EICLEAVRQNCNAIKCVQNQTEKICIEAVKQDWRMLEFVEEPTEEICMLAINQDGTAL
                     KYIENQTEELCLRAVEKNGAALEYVKEQTEEICIEAVRNSEFGLARVKNQTEKICMEA
                     VKHCSYNLKWVENQTEEICMEAVRQNGLDLKFVKNQTETICLRAVRQNGMALEFVKEQ
                     TVGICLKAVRQYGMALKFVKEQTEEICTEAIKQDKRALSFVKGDKEKYKALYDNNEPF
                     AKRYVRNVIEKENRALIKKGEENAKIKIAGKLYDRGMSFEDISDIVEIEISKLKVSLG
                     VF"
     gene            22610..23098
                     /locus_tag="KR250_000542"
     CDS             22610..23098
                     /locus_tag="KR250_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBH0066511.1"
                     /translation="MKITVLYQTRSGNTERVAKLIEEGAKKVDGVDVKLMRLDNIDLD
                     YLNESKAVIFGTPTYLANTTWEVKKWFDEDSKKVNLAGKLGAVFATCDYICGGPDVAI
                     LTIVGHLMVKGMLVYSGGGSLGKPFIHLGHVHSIEGPELQDEKAIIFGERIATKAKEL
                     FA"
     gene            23274..25202
                     /locus_tag="KR250_000543"
     CDS             23274..25202
                     /locus_tag="KR250_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBH0066512.1"
                     /translation="MVWHIEYEIFSAIIVIFLMVYFFRSKFIPTLQNKIYCALLIFSF
                     LFIISNILGSFCLNNIDKIPIFITFLLNQIYLLLLPIPAALVSFYVMAIIYQDIRYMK
                     KRLLFLSIPLIICIILSITNPFTHILFGLSKETGYIRGCGYVATFASFFFYAIYSAFL
                     SFKYRKAMHESKIWAIRGFLIVSIVAIILQAIFLQYLLTGTACACSLLLTYLSMQNRG
                     LIIDDLTGVLNRQSFIQELDLNINTEQSGFIITIALDDFKFMNETFGTKNGDIALKEV
                     GKYLIQIHDVNHVYRIGGDIFSIVVNSQLGIEPDDIIRKIEERFKKPWLVEEISFNLA
                     TSIAVVYYPENAETTEDVIVAIDFSIHEAKRSESRRVVYADASISEKIKKKHVIKNCL
                     KEALKNDGFEVYYQPIFSNSRGRFTSAEALLRLEHKELGFIPPDEFIPIAEQTGLINS
                     IGLVVFEKVCRFIASDEFEDLKLDNIAVNLSVVQCMQKNLADDLLYLMNKYNVSPTKF
                     KLEITETVAMGSFNIIKETMERLIDLGVKFALDDFGIAYSGVTNMLSLPFSVIKLDKS
                     LIWSMNEDSRHKLTVETIIALINKLNMKAVAEGVETIKCAEDLIAMECEYLQGYYFSK
                     PVPEDVFKNLLKENTFTS"
     gene            25379..25789
                     /locus_tag="KR250_000544"
     CDS             25379..25789
                     /locus_tag="KR250_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBH0066513.1"
                     /translation="MNKKKILVPIDGTERSMHSLEFIKGIFKKDEVEVEIMNVKELVF
                     IDGISLAEEIKNSENLGRRILDKAAEIMGEYDVKVHFTFGYPGDEIIRKAKEDNVDFI
                     VMTKSTKKGLTRMIGSVTASVVKQAKCIVMIVPE"
     gene            25811..26113
                     /locus_tag="KR250_000545"
     CDS             25811..26113
                     /locus_tag="KR250_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIBC"
                     /protein_id="HBH0066514.1"
                     /translation="MNKKIVAVCESERSLNSLKTAANDLGFDLVYEIQENDNIKNELS
                     IYDIEDASIVLFVTSDSIESIEKIDRFIDREFYEVDPKYIIEDAKSIVSEILIDLN"
     gene            complement(26330..27022)
                     /locus_tag="KR250_000546"
     CDS             complement(26330..27022)
                     /locus_tag="KR250_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HBH0066515.1"
                     /translation="MKSLNATEKIIDYIKLNILNGTFKINSKLPSERKIADLFNISRI
                     PVRNAINILCKEGILRAVPYSSPIVEGFKKVDLFDDGEIYKNHNIQEFYVESLRARQL
                     IESEATKLAILNATSKELQKIKYTYLKSIEELDKVSHGLIEECYDADLQFHKEIILAS
                     HNPIFIKYYELIPKTVSSNQYFGFKYRNSLKDMISHHNKIMIAFDSKDSYLGYTSMYN
                     HLEDVIQLFQHD"
     gene            complement(27048..28235)
                     /locus_tag="KR250_000547"
     CDS             complement(27048..28235)
                     /locus_tag="KR250_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBH0066516.1"
                     /translation="MKKISNQKLLLLSIVTFFWFSQYVYIPFQNPYLSACNVSTNMIG
                     IIIGAYGISQCLLRLPVGVFADSINKHKIFILSGIFFAGLASLIRVFSPGGNGFLIAN
                     ILSGISSSTWISYMVHYTNFFSTEHQQKATSTIILVNNLGMLIGFVMSTLFYEKLGMT
                     ALCIMSVLSSVIGTILALNIKNHNSKKIPEKISIKKYLSICKDKRLVFFSFMALIQQG
                     IQMSTTMSFTTQILKNLGSSSTLIGLSSIFYMVCAVASSGFASTKFCTKYGPKFWIPL
                     VFILVSAYCFLVPTIKNINIIFVLQVLPGISTGILLSYLTSESMKNVPKHQKSTAMGF
                     FQAIYAIGMSVFPMIVGKISEYTSIQYGYFILAIIAILASILSYTYYKKVDLTLNYDI
                     IQS"
     gene            28571..30448
                     /locus_tag="KR250_000548"
     CDS             28571..30448
                     /locus_tag="KR250_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBH0066517.1"
                     /translation="MDYKNIAQEILLNVGGKENVNEVTHCMTRLRFKVKSASKVNKDK
                     LSKTEGVITVVESMGQIQVVIGNKVKKVYDEVIKIVPQSNNVGKKNESQEKQGIINSI
                     LSAVAGIFTPTIPAIAGVGMIKGILSVLAMYYMNKNGVDIKETQSYIILNAMADSIFY
                     FMPIILGYTAAKVFNANKIISMVLGATLCYPTFTALMAGEESVRFLGLAVTKATYTSS
                     VIPIIIAIWALSYVEKVLEKYIPEVIKIIMVPTLSLVVMLPATLFLFGPIGIYIGNVI
                     NFSYKYIYELSPALCGAFVGGLWCVLVIFGAHRALLPIGISDVAQTGRQNLLAFAGAA
                     NFSQAGAALGVFFKTKNQGLKTISMSATITALFGITEPAIYGANLRLKKPMVCAVICG
                     AFGGAIMGMGGAYGNAFANQGVLTIPVYAEAGALGFLSYLGGCAIAFFGSAISTYLVG
                     FEDLEESSNKENSSIKVETKDGVIDITSPVEGECIELSEVKDDVFASKAMGEGIAVLP
                     TKGVITAPTDCEVASLFPTLHAIGLKLDNGAEMLIHVGINTVELNGKYFTKHVNQGDL
                     VKKGDKLISFDIDKIKKVGYDVTTPVIVNNTFDFGQVVSCKSSYVSTNDNIISLVLAG
                     N"
     gene            30758..32164
                     /locus_tag="KR250_000549"
     CDS             30758..32164
                     /locus_tag="KR250_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBH0066518.1"
                     /translation="MNTGFPKDFLWGASSSAFQVEGAWDKDNKGKTVADYNSFKKSHL
                     QADTKVASDFYHNYEEDIELMKELGMKTYRFSISWARIIPDGEGEINQKGLDFYNKII
                     DKLIECDIEPFVTLYHFDLPFKLVEKYNGWESRETVYAFERFAKICFKHFGDRVKYWQ
                     PHNEQNLIVRVEERINIYDETDSWKIDKMRAQMDYNLCLAHALAVNACHEMIKESKIG
                     AAVSSSVTYPLTSKPEDIYAARMNDNFKVYYMLDMHHYGEYPGYYMKYLEKRNIVPHM
                     EDGDKEILKKAKMDFIAVNYYRTNCAEALPEDSQHPFGLREGTVDFSMYGLFKMSMNP
                     NLEASEYGAAIDPSGLRVALNEYWQRYHLPVIITENGLGAKDILEDGKIHDDYRIDYL
                     RSHINACKLAIEDGVEMIGYCPWSFTDLLSSSQGFNKRYGLVYINRTDHEILDLKRIK
                     KDSFYWYKEVIENNGIVK"
     gene            32192..33040
                     /locus_tag="KR250_000550"
     CDS             32192..33040
                     /locus_tag="KR250_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBH0066519.1"
                     /translation="MYITKVLNNSLLLAKDDNDEEIILMGKGIGHNYKAGSELNEKDI
                     EKIFVLHDENIKKSIIQLATEIDAEYFGIAQMIIAYGIEKYNLKLMNHIYLALTDHIA
                     FAVRRFKEGIMIENHYLFEIKEFNPKEYDIGKYAITVFKEVLGLDLPEEEIGNIAGHF
                     INAQQDNPYSDRNKRSAKIVNAILQIVHYHFSIVYNKESFYYRRFVMHLKAFSQRFLA
                     NEPSREKIDFIYEQVQKNCKEEYECVKKVGAYMYKEYSRELPRQEELYLMIHIHKILG
                     ELCENS"
     gene            33542..34234
                     /locus_tag="KR250_000551"
     CDS             33542..34234
                     /locus_tag="KR250_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003020373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0066520.1"
                     /translation="MNKVLIIDDDKDLCILLKKSISIEQIEANYCHSGRDGLVELSKQ
                     DYQLVVLDVMMPGMDGFETMEQIRKYSSIPILMLTSKSDNYSKVHGLRAGADDYLTKP
                     FEITEFTARVLSLIRRYTRFNPKNEEPQQLSFKGLLIDLDQHTVTTNGEPMLLPPKEF
                     GVLLYCARHQGKILTKQQIYEAVWGEPYVYDDSNIMAIISRLRKKIELDPSSPIYLQT
                     IKGIGYQFNTEV"
     gene            34231..35166
                     /locus_tag="KR250_000552"
     CDS             34231..35166
                     /locus_tag="KR250_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBH0066521.1"
                     /translation="MKMDEQTIILFLLLLSIGLLFLSIFAFSKLGHIHKRLKDIEEIL
                     ADVQIGDENRKILIKPCDVMAPLVYQLNEIVYDYENKLLSLKKSDKASKQLMTSLSHD
                     VRTPLTTLIGYLDAVHSGIVIEQEREEYLEIARRRAYDLKDYIDVLFDWFRLNSDEFT
                     LSIESVEIAELSRNILKDWIPIFHEKKLNFEIDIPENRLMVNLDPDGYSRVVNNLVQN
                     VLAHSKARQIKITMSEDSRMVLLRVEDDGVGIARENLPHIFERLYKCDKGRSEKGSGL
                     GLSIVSQMVERMGGQVSVESEIGKYTVFTVSLPLI"
     gene            35314..36237
                     /locus_tag="KR250_000553"
     CDS             35314..36237
                     /locus_tag="KR250_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013273760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0066522.1"
                     /translation="MNNLIIETKNLTKQYGAQKSVSNLNIHVQKGRIYGLLGRNGAGK
                     TTTMKMLLGLTKPTTGEIKIFGQDIHNNEKKLLPRIGCLIESPGFYPNLTGTENLKIF
                     ARLRGIPKKNAVESALDVVGLPNNDKKLFSQYSLGMKQRLAIALAIMHDPQLLILDEP
                     INGLDPIGIAEVRTFIRDLCTERGKTILISSHILSEIALLADDIGIIDHGILLEEESL
                     AQLEEKNSKYIHFAVSDAAQAARLLETQLNLKNFKVADNYNIMLYDTDVSVAEINRAF
                     ILNNIEVSESHLCEDTLEDYFKKVTGGEGIA"
     gene            36221..36991
                     /locus_tag="KR250_000554"
     CDS             36221..36991
                     /locus_tag="KR250_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0066523.1"
                     /translation="MKELLDIISCEFSKLKRLKFILISILGACLFPIPATILIAKDNL
                     PFEQLFKLVVNFGYFLLLPIVLSIVASQLFFIERDNDVLKNLATVPVPKGKLALAKLA
                     VLLFISLFYSVAGLGATIIGGFIVGIVEGVAVKLGMSIAFGIMFFVTVLPVVVLIVYF
                     NRSYIFSIILSFVYAIFNFSISLNIINFEPNNPLLSVLPAPVIMRWWMAFWGDPTGEY
                     TVLRQPYLLSTPACVGILCLITIIAVLLICMIYKKQEN"
     gene            37001..37783
                     /locus_tag="KR250_000555"
     CDS             37001..37783
                     /locus_tag="KR250_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0066524.1"
                     /translation="MLDIITTEFQKIKRYNILWIGVVAVLFSALLAVFQVVSSHGSDP
                     LTYESFANGVIWNNFSLSFPFGITLIGGYLINREYTDQTLKNILTVPISLRKLLVGKL
                     IAVGGITVLLSLFSFLCTFILGLLFCNINISVILVVKSLVQILSVNLYCYVAVLPIIA
                     YFGRKQNAFLTGVGIAFVYGFCGVFVAGRNLTDFYPITAGLGLVGYNNGAGTVYQPLI
                     GAMTLIVILILTTILLVFTPNYDKVMTISKKKGIKHKKYSHK"
     gene            38160..38696
                     /locus_tag="KR250_000556"
     CDS             38160..38696
                     /locus_tag="KR250_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HBH0066525.1"
                     /translation="MNLKGTKTEKNLMAGFAGESEARNKYTYYASKARKEGYNQIAAI
                     FEETANNEKEHAKLWFKLLHDGMPSTEENLKDAAAGENYEWTDMYAKFAKEAREEGFD
                     KIAYLFEAVGKIEKEHEERYLKLLENLNEGKIFKRDEEVVWQCQNCGHVYVGTEAPEK
                     CPVCDHPKAYFNIKAENY"
     gene            38728..39117
                     /locus_tag="KR250_000557"
     CDS             38728..39117
                     /locus_tag="KR250_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBH0066526.1"
                     /translation="MKFSKQRELILNEILNNPVHPTADYLYENLKKDNPNLSLGTVYR
                     NLAQLTEHGFIRKVSIPGYPDRFDGRIDNHYHIICEVCGEVYDLESEVLNNLQELISE
                     ETDIKITSYNISFKGICNNCKRCSQVG"
     gene            39154..39540
                     /locus_tag="KR250_000558"
     CDS             39154..39540
                     /locus_tag="KR250_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin"
                     /protein_id="HBH0066527.1"
                     /translation="MCSEQKFFICETCGNLVGMIQSGGVPIFCCGKPMKELVPNTTDA
                     AVEKHVPVIEVDGNNVTVKVSSTTHPMTKEHHIAWVYLMTEQGGQRKCLAVDGEPVVK
                     FALNDDDKVISAYAYCNLHGLWKAEL"
     gene            39667..41109
                     /locus_tag="KR250_000559"
     CDS             39667..41109
                     /locus_tag="KR250_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBH0066528.1"
                     /translation="MSIYKCSVCGYIYDESKNDKTWDELSEDWECPVCTKGRSYFGKI
                     STVYYEEDEKIAEDIVEDESKLNTEKEGDLNYLSTYLRRDDEVEKHMDIIHEMAVTGK
                     SIIEPMRTKLPVISWDDILIMGAQLNPLPLNEHDEVNTTTIIGKKAKKPMIIENPVYI
                     SHMSFGALSKELKIALAKGAAQNKTAMCSGEGGILPEEKEASYKYIFEYVPNKYSVTE
                     ENLKNSDAIEIKIGQGTKPGMGGHLPGEKVTEEIAKVRNKPVGQDVISPSCFEEIQSK
                     EDLKKLVDELREVSEGRPIGVKISAGHIEKDMEFIAYAKPDFVTIDGRGGATGASPKL
                     LKDATSIPTIFALYRARKYIDTHGLDIDLVITGGLRISTDFAKAIAMGADAVAIASSA
                     LMAAACQQYRICGSGKCPVGVATQDEELRKRLHIENSANRVANFLNVSLEELKTFARI
                     SGHKDIHDLSVDDLYTVNSEISNYTNIQHV"
     gene            41406..42221
                     /locus_tag="KR250_000560"
     CDS             41406..42221
                     /locus_tag="KR250_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBH0066529.1"
                     /translation="MERLKIQVLVDNNTYIDRYFVGEPAVSYYIEIDGNRILFDSGYS
                     DVFISNAEKLNIDLGNLTHVVFSHGHNDHTRGIQFLENRYDLSTVELISHPNCFIPRK
                     HGEKSIGAPFSAEEIKNIFMYNPKDKPFNLSKNCVFLGEIPSINDFEKRAKIGKCKIG
                     DLWEDDYVLDDSAIVCKTDKGIFIVTGCSHSGICNIVEYAKKVCGDDRVIGILGGFHL
                     FELNNRLDSTIQYLDKEEIRMLYPCHCVSFKVKAKMNEILNINEVGVGLTISI"
     gene            42823..44007
                     /locus_tag="KR250_000561"
     CDS             42823..44007
                     /locus_tag="KR250_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0066530.1"
                     /translation="MQKSVKKAKVTGSMLAIFGVASVLFSSHAGGGFATGNQETQYYV
                     QYGWTAPLMAILAMIILTATMREVIIMYNNNNCRNYKDLFCELWRPYPKLEIIWEIYY
                     YLMVLIAVSAVIAGAAAVFQSIGVNYFVAVFIIGVVLLVFTIFGAMLVSKAATAMTIA
                     ILVCTLTIFIVGIKAKVPEITEILSNRTSFTPGYIKPILNTFIYAGFQSVVIPTLAGC
                     SRPLLKNSKEATKAMIISFVMNAIALGLAVTMLMGWYREIIAAGQTTLPTLYVAGQSG
                     NHTIYIIYNVALFLCLMSTGVTTIFGLVNRFEDHKALSFLSSRMKRRVFTACAIMVVS
                     MLISLTGLSNIVKYGYGYCGYLGLFTIVIPFLTLGHYKNKKFAKENPEAKWPAELNEN
                     VN"
     gene            complement(44384..45493)
                     /locus_tag="KR250_000562"
     CDS             complement(44384..45493)
                     /locus_tag="KR250_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBH0066531.1"
                     /translation="MDFKSLGISENTINILKKSGITTPTPIQKESIKLIKEGKDVIAE
                     AQTGTGKTLAFLLPIFENISLDINDIQVLILSPTRELAIQITEEAMKLKESKDVSILA
                     AYGGKDIGSQIKKLKGNIHMIIATPGRLLDHLNRKTIDLSKLKTFVLDEADQMLLMGF
                     KNEVEAILKETSNKKQTLCFSATINSQVKKLAYRYTKNPVVVSIQKEEITLNNIKQEV
                     VETTDRKKLDALCKVLDEDNPFMAIIFCRTKRRVDNLEEALAIRGYNCQKLHSDIAQS
                     KRERIMKSFRNLDIQYLIATDVASRGLDISGVSHIYNYDLPETPEDYIHRIGRTGRAG
                     EEGYTCAFIDPKNERMLSEIETAIESKISRRIIEL"
     gene            45955..47148
                     /gene="nifV"
                     /locus_tag="KR250_000563"
     CDS             45955..47148
                     /gene="nifV"
                     /locus_tag="KR250_000563"
                     /EC_number="2.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocitrate synthase"
                     /protein_id="HBH0066532.1"
                     /translation="MCVISKDRAKEIKIVDTTLRDGEQTAGVVFANREKIMIAEMLSD
                     LGVDQIEVGIPTMGGDEKNVIKHICSRNLKSDIMAWNRAVIKDVEESISCGVDAVAIS
                     ISVSDIHIENKLRTSRGWVLENMAKTVEFAKKNGLYVSVNGEDASRADIDFLTEFINV
                     GKQAGADRFRYCDTVGVMNPFSIKNAIETLYERTNFDIEMHTHNDFGMATANALAGIA
                     AGANYVGVTVNGLGERAGNAALEEVLMALKCVYKCDLNNIDTRKFRGICEYVAQASGR
                     ILPTWKPVVGDNMFIHESGIHADGALKDPHNYEPFDPSEVNLERKIVIGKHSGRAAVV
                     NKLSEYEMYISPENATKLLNAIRATSIRLKRSLMDKEILQLYCDILAHEKGTTEEEAV
                     RGSYI"
     gene            47173..49098
                     /locus_tag="KR250_000564"
     CDS             47173..49098
                     /locus_tag="KR250_000564"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HBH0066533.1"
                     /translation="MGDNIVYKIIKKHIVDGEAVAGSSIGIKIDQTLTQDSTGTMTYL
                     QLEAMGIDKVKTKRSVAFVDHNMLQQGFENADDHKYIQTVADKYGVYFSKPGNGICHQ
                     VFLERFSTPGDTLLGSDSHTPTAGGVGMMAIGAGGLDVALAMAGGAYYIKAPKVCKVN
                     LVGKLNNMVSSKDIILEVLRKQTVKGGVGKVYEYGGEGVKSLSVPQRATITNMGAELG
                     ATTSIFPSDEKTLEFFKSQGREEAWIELKPDADAVYDEEITINLDELKPLAAKPHSPD
                     NVDEVENIGKIKIDQVAIGSCTNSSYEDLMKVAQILKGNKVHKDVSLVIAPGSRQVME
                     MIARNGALADIISAGARILENSCGPCIGMGQSPGTDSVSLRTFNRNFYGRSGTLSAQV
                     YLVSPEVAAVSAIKGVLTDPREFDIKFTNLDVNEFLIDDSMIIKPADVGSDVEVVRGP
                     NIKPFPLNTELSQSIGGKVILKTEDNITTDHIMPSNAKLLPFRSNIPYLANYCFNTVD
                     TEFPQRAKDNNGGFIVGGDNYGQGSSREHAALAPLYLGVKGVIVKSFARIHKANLINS
                     GIIPMEFCDEKDYENISLLDNLEIPNILDNLGSGILEVKNTTKGTSFKVKVELSAKEV
                     DVLKAGGKLNYTKNQAN"
     gene            49120..50115
                     /locus_tag="KR250_000565"
     CDS             49120..50115
                     /locus_tag="KR250_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HBH0066534.1"
                     /translation="MYKVTLIPGDGIGPEVAKAMKKVVEATGVEIEWEEVNAGEAVIE
                     EYGTPLPEYIIDSIKKNKIAIKGPITTPVGKGFRSVNVTLRQALDLYVNLRPIKSFKG
                     IKSRYEDVDLVVVRENTEDLYAGIEHKIGDYAAESIKIITRSASERIVDFACNYVKDN
                     KRKKVTAIHKANIMKMSDGLFLDVFREVASKHGVEYDDLIVDAAAMNLVLNPENYDVM
                     VMPNLYGDILSDLGAGLVGGLGIIPSANIGKDCAIFEAVHGSAPQIAGQNKANPTALI
                     QSSVMMLRYLGEYENAQKIETALEKVFLEGSKLTVDLGGSASTTEFADEVCKYIV"
     gene            50441..50914
                     /locus_tag="KR250_000566"
     CDS             50441..50914
                     /locus_tag="KR250_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBH0066535.1"
                     /translation="MVVSVEVCLIKTLDSNILREVGTLSRAVNSINDIKYKELKLQKG
                     QFTFLTRICENPGINLVELSNMLKVDKATTTKAIQKLIKAGYVDKKQDKFDKRGYNLT
                     PTDKSLEVYELIIEEENRSIEICFDNFTDEEKQVVTKLLEKMSKNVENEWFKVKR"
     gene            50948..51442
                     /locus_tag="KR250_000567"
     CDS             50948..51442
                     /locus_tag="KR250_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBH0066536.1"
                     /translation="MIRKANMNDLESIMQIIKSTVEEMKTYNNTQWDENYPLEKDFVS
                     DIKKQDLYIYEVDGEVAGFICLNYEEPEEYLGLNWSSNKKAMVIHRMAVNPNFRKTGI
                     ASKLVDFAEKLAVENNVSYLKSDTYSINSKMNLLLTKCGFIKIGEMSFLGKEKSFYCY
                     DKIL"
     gene            51539..52060
                     /locus_tag="KR250_000568"
     CDS             51539..52060
                     /locus_tag="KR250_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBH0066537.1"
                     /translation="MIGFLKKRRSIRKYKDVEVEKEKLDKILKAALLAPSSKGLRTWE
                     FIVVDDKEKLINLSQCRTKGGGFFLKNAPLAIVIIADKEKNDVWIEDASIAASYIQLQ
                     AHELGLGSCWIQVRNRMHDDNIEADKYIREELKVPSKYSVECIISIGYPDEEKKAYGD
                     SDLDYKKVHFNNF"
     gene            52249..52560
                     /locus_tag="KR250_000569"
     CDS             52249..52560
                     /locus_tag="KR250_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBH0066538.1"
                     /translation="MGIFKTKMDEDWKVNYIKEFNEMRDSYESKLQKKQFEVDSLKSE
                     LDRLRSYKNSLKPKEKQITDDDINNIKSLRRDGLSYKEISNQTSWSKATVSRVLNGLY
                     D"
     gene            52588..53274
                     /locus_tag="KR250_000570"
     CDS             52588..53274
                     /locus_tag="KR250_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBH0066539.1"
                     /translation="MKYSYKKCIIDSILLMFIVQILRMILNYVLLSQFEFTLENFNII
                     NLISFTLVGLSLILFLKDNSLYNKVRNRKITEAFEENKNNILIEKCKLILFVVVLSLA
                     IIVTYCTKGYVLFNVTMMTLSVLIVPIFEELFFREYIWNYLSNFIKSKSKIICITSIL
                     SGIYNIGYIDVIRNYVILYNNSSYTFEVIISKIMIGTVFGIVLGLVKYRFRDVGFCIL
                     LRSLFAIFIR"
     gene            53506..54390
                     /locus_tag="KR250_000571"
     CDS             53506..54390
                     /locus_tag="KR250_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HBH0066540.1"
                     /translation="MKFVTFCSSNEEKIGVFNSETNSIYEINSLGLSKLYTDMNDFIE
                     NVSTGDLEKIKNNSFENAKCYKLEEVKLCSPIVRPKKDIICLGLNYKDHVNEIPDGVI
                     KNVVMPDYPIYFSKRADKIIGVDDKISLHGDLVEKLDYESELAVIIGKEGINISKEDA
                     YEYIFGYTIVNDISERALQDKHVQWFRGKSLDTHTSMGPCIVHKEEFEHPLKLDISSV
                     VNGEVRQDSNTEYFIFDIPTIINDLSRGMTLKPGDIISTGTPAGVAMGMNPQVYLKHG
                     DIVECKVEGIGVLKNIVD"
     gene            54489..55664
                     /locus_tag="KR250_000572"
     CDS             54489..55664
                     /locus_tag="KR250_000572"
                     /EC_number="2.1.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolecarboxamide
                     formyltransferase"
                     /protein_id="HBH0066541.1"
                     /translation="MARELELKYGCNPNQKPSKIYMKNGELPIEVLNGKPGYINFLDA
                     FNSWQLVKELKEATGLPAATSFKHVSPAGAAVGVPLSDTLKQIYFVDDLELSPLACAY
                     AMARGADRMSSYGDFIALSDVCDKETATIIAREVSDGIIAPGYTDEALEILKGKRKGN
                     YNIVKIDENYTPEPIETKDVYGITFEQGRNEILINEDLLKDIPTDNKIFTDSAKRDLI
                     IALITLKYTQSNSVCYAKDGQVIGVGAGQQSRIHCTRLAGNKADTWYLRQHPKVLNLK
                     FKKDIGRPDRDNTIDVYLSDDYMDVLADGIWQNFFEEKPEPLTGEGKRAWLKTLTGVA
                     LGSDAFFPFGDNIERAKRSGVSFIAQPGGSIRDDNVILTCNKYNIVMAFTKNRLFHH"
     gene            55792..56646
                     /locus_tag="KR250_000573"
     CDS             55792..56646
                     /locus_tag="KR250_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0066542.1"
                     /translation="MALNIRNYSLLCNQGTAKFNEDVVGINPFGAWVLDGATGLNGKN
                     LVSTESDARWYVQWWNRYLYKNINKEESLKSIINTGIIEVKKEYELRLNGFKVEKLDL
                     PSSSIVVVKFHENKVEYFLLGDCALFIKNGDSKVIKDRSICKFDNTVFDEMSKLSNLE
                     EMAFDEIRTSVMDTIVSNRLKKNTKEGYWILDFDEEAVENSIYGYIEIKKDFQLMLTS
                     DGFTSACDRYNLIKEEELIRIAEKFGIGYIHNKVRDFEDNDYKAVKVPRFKVKDDSSC
                     LYLDIYND"
     gene            56690..57925
                     /locus_tag="KR250_000574"
     CDS             56690..57925
                     /locus_tag="KR250_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBH0066543.1"
                     /translation="MKILHIITQKPNSTGSGIYLSGMIKGFEKIGHKQAVIAGIDVND
                     DVNCFPSEVSFYPVKYNCGELNFPVVGMSDSMPYESTRYKDLNIDMINRLKYQFKVNI
                     DKAMNDFKPELIICHHLYLLTAFVREMVKDIKVMSICHGTCLRQLNTIDLEKEYIIAN
                     IRKLDLIFALHENQKYDIIKTFGVSQSKVVVIGSGYNDDIFYNKNYKIKDDKIKIVFA
                     GKICKSKGLIPFIKAISKLKYSKDLIEVNFAGTGSDIESYNEIVKLASKSPFKMNFLG
                     KLEQRDLAELFNRSQIFVLPSFYEGLPVVVLEALSCGTDVITTDILGVKEWIGSEINN
                     SGKIEYVSLPFMEKEGIPKDEELYDFENNLYNAIDSKIQSLLNNSNKKTSVDMSKKTW
                     DGLAYRINEVILMDELCLI"
     gene            58026..58967
                     /locus_tag="KR250_000575"
     CDS             58026..58967
                     /locus_tag="KR250_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp25"
                     /protein_id="HBH0066544.1"
                     /translation="MRISKKVLALGISALLLSVSFPTSINALDKIENIQGVDKYETAG
                     LISDKQDFTTAVLINADSTMVDGIAASGLAGVNNAAILLTNKDDIPEATLQRLNRVTK
                     IYVIGGENSISKDVEKMLLMRRMQVIRIDGVDRVDTSYKIAGEIEKIKNSDKMFLVNG
                     FKDEADAVSVASVAYRDGAPIILTKDIPSAEEDTDLDPWFGVSPVPVYAIGGESTLSD
                     YIVSRYRATRIGGVDRYQTNKNVIEKFYNGAKEFYITSGDDLVYALVASPLAKNAPVV
                     LVSNKSDKSILSGASKVTAIGISDKSIIEQCLDAVKK"
     gene            complement(59186..59539)
                     /locus_tag="KR250_000576"
     CDS             complement(59186..59539)
                     /locus_tag="KR250_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HBH0066545.1"
                     /translation="MDRKRELKQLYKEMKFDTGVFIIKNDITKKIYLGKSNDIKSKFN
                     SLKFQLGAGSCMIKELNNEWKKYGEKAFTFEVLELIKHDDNKTEKDYLEELDILEMVW
                     LEKLKEDNTYEIYSI"
     gene            59835..60746
                     /locus_tag="KR250_000577"
     CDS             59835..60746
                     /locus_tag="KR250_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HBH0066546.1"
                     /translation="MNKKIELIGNFASDWIRYSDYEYKITEEDELYIVPTEDAVFTMY
                     NPFDVADDIIVDIIRIGQEALQDISKETKEMKLKKEVLEFVKKYGLLGLIYSSVYNRD
                     IVGEERVLMIEKNYITKENILSTDEYVNMFIPFASDEDVYFKKYRRGIDVSKREESPK
                     FFGKRPLILDLVFSKFYTEQLDWIVSFAKDMCKHFNQICVYKNTSNLLTESVTIMSGG
                     FNPQKIGFSISQLDKPIIAWEFDSLKTAIETIYAFAITSEPSSLNKCKYCGKIYIPTN
                     SKSQYCSPSCRNCSNVKKSRSRAKKDK"
     gene            61006..61440
                     /locus_tag="KR250_000578"
     CDS             61006..61440
                     /locus_tag="KR250_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0066547.1"
                     /translation="MISPIILSSINQNLKEIERNELLETNIESGDYGLALSESDVKDI
                     INSRDNTLKGYGRIELDIKVTKQLIENIYTSQYTNVDNYLEAINDMQEIFYYLKNETD
                     DKICDDEIIEILGEFYEKFSGNMDNVRGEADEFAKKFKFGEV"
     gene            61463..62998
                     /locus_tag="KR250_000579"
     CDS             61463..62998
                     /locus_tag="KR250_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0066548.1"
                     /translation="MLNNLSNININENNLIKNQYSISLLKECLNCKVIDEREVYNIQQ
                     EISLILMDLIKKYTNGQSTSVKTEVAEKLLISIWYAIDAYINKLGDIEKRVVELTAKN
                     VKKVYLGGIEILEEELVRLKDFYKLMMSRKLDTNSIAYNDTLMELSSFFKSYIVKFEA
                     QDVPVSIDYPLALDDMDVKGIYYVKNYIESIDIENRFCNLFKKKDREKLFYDYGVTYK
                     IDCDAILVNLFELIINNCIFSTILGNEVTNLEISKYEYEFLEKQFKNMYNNITEDKVI
                     DSEESNIIELIEVDDSFNTEVDSNLDYNDFEEINREIRINKKITLLLMEAIEVLIKRL
                     SIEDEDVIRYIKKYEKIFIESVLSSIKSNTFKSMVTVSKNKKEHLEDYIVDDESKLDD
                     ESFREIFNEILDSTSIIEKIRIIKENINAKKDFIDILESECLFGEEYLMLFASLSELE
                     LAILGSVVFYEDIRMREINILEFMLNEKTETTLWKIEYIKFMKRQSEDKIYSIEEHMN
                     KIN"
     gene            63082..64449
                     /locus_tag="KR250_000580"
     CDS             63082..64449
                     /locus_tag="KR250_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBH0066549.1"
                     /translation="MKEYIINNVEEIREELIDLSKKIWENPELAFEEKYASSIQKEYL
                     KSKGFKIEEVENLPTGFIASFGEGKPVIGILGEYDALPELSQCVSAERKPLVEGKAGH
                     GCGHNLLGVAGVGAVVSIKKLIEENKFNGTIKYFGCPAEEEGGGKTAMCINGCFDDVD
                     CAFTWHPFDINAPWRGGSLANLSVKFKFKGITAHAAQAPHNGRSALDAVEIMNVGANY
                     LREHVIDSIRMHYVITNGGGRPNVVPGFAESWYFIRGKKAKDAEHVLDRLIKVAQGAA
                     MMTETEMEYKVTDGIYDYIPNQCLTDLVYDNMVFVGCPKNTPEEEEFAKKLCDTLTRE
                     ERLGVATSLQNDKSIVESYIHKEIVDGDKDKGLAGSTDVGDVSYVIPVAQFAMAAWPV
                     GIASHTWQSCSSAGSNIGFSAMINSAKVLACSAYDVFMDTKIIDEAKIEFDKSLDGQK
                     FKPLV"
     gene            64484..64708
                     /locus_tag="KR250_000581"
     CDS             64484..64708
                     /locus_tag="KR250_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HBH0066550.1"
                     /translation="MREKVEKVLEEKIKPVLQRDGGDVELIDVNENGVVLVRLQGACS
                     GCPGATMTIKAIIENVLVSEVPGVTQVLGV"
     gene            complement(65158..65556)
                     /locus_tag="KR250_000582"
     CDS             complement(65158..65556)
                     /locus_tag="KR250_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03987 family protein"
                     /protein_id="HBH0066551.1"
                     /translation="MNYKLILAIVFITSALIFYTIGVFGERKAKILKKKHVIIFWLGF
                     IFDTLGTFTMSNIANSHTFEVKSALSQNLHSITGLLAIVLMLFHASWATFVLYKDDEE
                     KKKFFHKFSIVVWTIWLVPYFIGMFIGMAG"
     gene            complement(65559..65960)
                     /locus_tag="KR250_000583"
     CDS             complement(65559..65960)
                     /locus_tag="KR250_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBH0066552.1"
                     /translation="MLLKELYSKTMYTVEENFKENGLTHQQIIVIKLIAHNQELTISQ
                     LCDEMSLAKGTVSGIISRLEQIGYIEKFKKSNDKRNTYVKFTTTGFEFATNFKIKMQE
                     SFDDIFKNCDENELSDLVKNLRNILAKVKER"
     gene            66320..67306
                     /locus_tag="KR250_000584"
     CDS             66320..67306
                     /locus_tag="KR250_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0066553.1"
                     /translation="MLKDILKKLLQFILVMFLLSFIVFYMARLAPGDPLISYYGDGVE
                     RMSTQEKENAMKKLGLDEPIYSQYIKWISNASKGEFGISFKYKQNVTSVINDVYINTI
                     ILGGSGYILTFVLALLLGIFCTLHEDKLIDRIICKLGTITNCIPSFWVALVLILIFSI
                     NLSILPSSGAYSMGEESSILSRISHLILPLTVLILSHLWYYTYMIRNKLLEEIREDYV
                     LLCKAKGLNNRTIVFKHCLRNIMPSYISIMAISIPHILGGTYVVEKVFSYPGLGTLCF
                     ESAKYHDYNMLLVLCLITGALVVFGNMLAQIINNKIDPRMKYDRGDTNEATI"
     gene            67290..68201
                     /locus_tag="KR250_000585"
     CDS             67290..68201
                     /locus_tag="KR250_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0066554.1"
                     /translation="MKPQYSDFEIVGENYILVAEDEHIETKKTLSKKIKELPYISIII
                     LSIIVIGSVFSSFIMTHEPTYMDLASSNLAPNKTFLFGTDSMGRDIYSMIWYGGKISL
                     FIGLFSTVISTVIGIVYGSISGSASELVDDAMMRFTEIILSIPSILIIIFVQAILGNS
                     NPVSMSIVIGITSWMNISKIVRTEVRQIKNSEYILAAKSMGGGFFYVLKQHLLPNFVA
                     SIMFMVVTNIGAAIGTESTLSFLGIGLPIEIVSWGSMLSLSEEALLSNRWWIILIPGI
                     FLVTTLVCITNIGNYIRKNNNKKSSNL"
     gene            68314..69882
                     /locus_tag="KR250_000586"
     CDS             68314..69882
                     /locus_tag="KR250_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBH0066555.1"
                     /translation="MKLKKLKVLSLVMILSLMAGCSSGGDKDKKADTPKDGKVLVYGS
                     NDYTSINPALYEHGEINSLIFNGLTAHDENNKVVPCLAKDWKFDEATNTYTFNLRDDV
                     KWHDGEKFTANDVKFTIETIMNPDNASEIASNYEDITKIDVVNDNTIKITLKAPNTAM
                     LDYLTVGVLPKHALEGKDIATDEFNQKPIGTGPFKLEKWDKGQSITLVKNSDYFVKEP
                     GLDKVVFKIVPDDKAKAMQLKSGELDLAQITPKDMSNFEKDEKNFKVNIMKTADYRGI
                     LYNFNSKFFKDKKAKGLPNALSYAIDRKAIVDSVLLGHGVPAYSPLQMGPYNNPDIEK
                     FEYNPEKAKQEIEKLGWKLGSDGIYEKEGTKLAFEITAGESDQVRVDMAKICAQQLKE
                     IGVDAKAVVVTETDWANQDAHLIGWGSPFDPDDHTYKVFGTDKGANYSAYSNPTIDKI
                     LQKARETEDKDEKLKLYKQFQVEMTKDMPYTFIAYIDAIYVGKPNIKGLTPDTVLGHH
                     GVGVFWNIADWTIE"
     gene            69957..70982
                     /locus_tag="KR250_000587"
     CDS             69957..70982
                     /locus_tag="KR250_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0066556.1"
                     /translation="MEHLLEVNNLSVSFKVEEGEVQAVRNVSFNLKKGETLAIVGESG
                     CGKSVLCKSLMRILPYNGYIKNGEVLLKSSDLVKKSEKEMEDIRGKNISMIFQDPMTS
                     LNPTISIGKQIAEAVIIHQGISKSEAKKRAIELIELVGIDNPEKRFKQFPHHFSGGMR
                     QRIVIAIALACNPDVLIADEPTTALDVTIQAQIIDLIKDLQHKIGLSIIFITHDLGVV
                     ATIADRIAVMYAGKIVEIGTVEDIFYDPRHPYTWGLLGSLPTLDSQDDYLYNIPGMPP
                     NLLNPPKGDAFAIRNKNALKIDYEKEPPMFKINDTHSAATWLLHPDAPEVDVPVRVNC
                     GRVISNE"
     gene            70975..71919
                     /locus_tag="KR250_000588"
     CDS             70975..71919
                     /locus_tag="KR250_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009527644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0066557.1"
                     /translation="MNREKILEIKNLKQYFHLDKSTTVKAVDDISFDIYKGEIFGLVG
                     ESGSGKSTTGKTIIRLHESTGGEVIYKGNCISDKKTYKFIKKDVNKSMQIIFQDSTSS
                     LNPRMTIADIISEPLKIQGICKNKTDRMNKVYEMLKLVGLDRSYANKYPSDFSGGQRQ
                     RIGIARALSVDPEFIIADEPIASLDVSIQAQIVNLFKKLQQEKNLTCLFIAHDLSMVR
                     HISDRIGVMYNGKLVELADSNELYNNPIHPYTKSLLSAIPVPDPRYAKSRNRIEYNSN
                     GYDCSNEKSLSWIEVSDGHFVYSSKSEINKYQQNLKVV"
     gene            72278..74935
                     /locus_tag="KR250_000589"
     CDS             72278..74935
                     /locus_tag="KR250_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBH0066558.1"
                     /translation="MIYKKDKVMKVLESSYRENEKGITANQLSEKTGIARNNISTYLN
                     QLFNEGMVTKIKGRPVYYIPANLNLNKNELLDTQSELLGIKKGTKVENKIKKDSFETL
                     IGKEHSLRPIIEKCKVAMLYPTNGLHTIIYGETGVGKSMIARYMYDYSIDSGIRKNKA
                     PFVTFNCADYANNAQLLMGHIFGVEQGAYTGAESSRKGLLEIANGGILFLDEIHRLPA
                     EGQEMLFTFIDKGTFKRLGDATKERESKVLIICATTENPTSTLLDTFNRRIPMKIEIP
                     SLRDRTMIERMELVKNFFKEESKSISISIKVHKEIIKSLLLYDCKNNVGQLQNDIKLA
                     VANGYLRYKRDNKEPVYIEKKYFDKSINEVSDDYREKSIKADEVITHGIEYFVFTSLG
                     EEEVINYNDINIINAVNTNLNKSVEMNIADASLFNNVKFKSMCESIRQIIKEDEEIDI
                     SESIFKSMAIYIKSVLDKSSSTNNIDLNQIRRNNRSEFKTALKIVGIIENEFDLFLSI
                     ETVAYITLFIVQAKQDEQNKIVNNINIIVAMHGETTASSMVKAVGDIIGKCSAISFDM
                     KLDKSYDEVIVDFKNLINNIDNKDVLLFTDMGSLNSFDEIIKKEKKCGVRVIPMVTTL
                     TVLEAVQKANMGLPLNDVYNSITNTRKHYFGTNEIQNKENLSKTIIIASHVSEGVDNK
                     TRKILEEKMSRYLDGIDIISVPYKTEKDLSLNITKLKESSNIVAVINEQRINIRGIDY
                     ISKKDIDKDENINKLKNIIKISIGYDDVVEGLKTSLKSSNYNRIFKDIKYVSDELFLV
                     FNIEKKYDKVIGLMMHLAFMVDGLIGNTREIEKLDKEKTLDYHKSLSKIKDIVSQLDK
                     KYNIEINEKECYQILLILEYAEIIEKDYQ"
     gene            75151..75780
                     /locus_tag="KR250_000590"
     CDS             75151..75780
                     /locus_tag="KR250_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0066559.1"
                     /translation="MQDKILRDKKANDPWARMTTRNGFSADEIISSFQKAIRRNMVEE
                     ACEFAYELYISSPELEDKLWRRILTISVEDIGMGDPSAVIIINNLNQVRKEYSYADGD
                     RPLFFIHAIRYLCACEKDRSSDLLKNIIIKGFAMGKVPEVMDVALDKHTYRGLEMGRD
                     SFHFLNEASIVIPEKEVDNDYKERYLKILEKYKQEEVIDTAFKFNHWQY"
     gene            75915..76241
                     /locus_tag="KR250_000591"
     CDS             75915..76241
                     /locus_tag="KR250_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBH0066560.1"
                     /translation="MNILLVCANGASTGVLVEKMKSFCSEHEKLKTKTINIEATSFEN
                     LKSYIEANDTDVVLVAPQIRFKEDDVVEACKNYKIAVGLIDTKHYGRMDAPSVMKSAI
                     ELYKNR"
     gene            76257..77546
                     /locus_tag="KR250_000592"
     CDS             76257..77546
                     /locus_tag="KR250_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBH0066561.1"
                     /translation="MEALQNNLRRFLLPIAQKIEKQRHLQAIKEGMISITPIIIVGSL
                     SMLFMALNNMLPEGSAKTLLSENMDTLLIPNKFTMSLLSIYSAFFIAQALAKKYNLNH
                     VEIGMTAVVAQLVVCGQVVDGVLDTSYLDAQGLFVSILVALLVVDITKFMNDKNLVIR
                     FPKEVPSVVNKSFRNLTPMIVCIMLFTAIAAITKNVSGQPLPAIIMNFLAPAISSVDN
                     VFAVTIILFITQLLWFFGLHGAAITSSIWMPIAATYMAENATLIAAGGDPKYVFTIGF
                     YYGFLQVTGSGITLGLVYLMSRSKCKSFNSMGKVVILPSLFGINEPVIFGTPIVMNPY
                     MFVPFVFGPVLVGALNFMALKVGLVGLPIAEPPGFLPPGVAAFLMTLDWKAIVLVFAS
                     IILMTLIYYPFFKIMEKEELNKNAELATTLDDDSFDF"
     gene            77570..77899
                     /locus_tag="KR250_000593"
     CDS             77570..77899
                     /locus_tag="KR250_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBH0066562.1"
                     /translation="MVPEMEEKVFSIISEAGDAKSDVMMSLKEIKKGDYNKAKHLLNS
                     ASEKIQTASKHHLELLSNAMNSEDSGTDFLVVHSEDHYSNALFAHSLVSELVDIFEMM
                     DPRINKN"
     gene            78047..79375
                     /locus_tag="KR250_000594"
     CDS             78047..79375
                     /locus_tag="KR250_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HBH0066563.1"
                     /translation="MEKFSVVIAGGGSTYTPEIILMLLDNLDRLPLRAIKLYDNDEER
                     QNKVAKACEILIKEKDPNIEYLATTCPKEAYTDVDFCLAHIRVGKLEMRELDEKIPLK
                     HGVVGQETCGPGGIAYGMRSIEGVLENIDYMEKYSPNCWMLNYSNPASIIAEAVRRLR
                     PNSRVINICDMPIGMEHNIARIAGLKSRKHMDIRYFGLNHFGWYTSIKDKKGNELLPK
                     LVEHIKEYGFINGEEGMKNDKKDSWFETNLFTKEIVKTDLTTIPSSYLKYYLFPDYVV
                     NHSDVNYTRANEVIDGREKEVFGSCALIEKQGHSKGSGLKIGIHAEFIVDLATALAFN
                     TQERMLLIVQNNGAIENIQDDAMVEIPCIVGKDGYEPLSIGKIPTFQKGLIEQQLAVE
                     KLVVDAWIEKSYNKLWQALMLSKTVPSATVAKELLDDLIEANKNYWPKLN"
     gene            79692..80552
                     /locus_tag="KR250_000595"
     CDS             79692..80552
                     /locus_tag="KR250_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-ADP-ribose hydrolase"
                     /protein_id="HBH0066564.1"
                     /translation="MAMKWRDYADDVNLFEDFDKTIKPLTDEQRRKNINTLIAYFSKE
                     VPSKIYNLSNDEIKPRDILRGLLNVYPPKEIAPEILNMLHNLLLIECEERELVDVNDI
                     EEVEEGIAIWRGNITNLRADAIVNAANNKLLGCLQPLHLCVDNEIHSCAGPRLREDCD
                     KIIKKQGHLEYTGDAKITRGYCLPAKFVVHTVGPIVSGGQPSKEQEKQLLHCYKSCLN
                     TIKEIDEIKNIVFCGISTGVFGYPKKEAANLAVSRVRLWLKENPEKNLKVVFNVFTEE
                     EEEKYRRIFK"
     gene            80757..81737
                     /locus_tag="KR250_000596"
     CDS             80757..81737
                     /locus_tag="KR250_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBH0066565.1"
                     /translation="MKNSFKNCISLRNKFIIISIVFLLILLIVSYIFGKLVYNSSVGL
                     EQLIKNEDIVKIFSERKDKPLEKLKNYKTSELMIQSSNNYKLESLFITSNIKTRDTMI
                     LVHGIGSSYYEMLKVAYRYLDKGYNVLVYNQRNTGNSGGDNYTFGLYERYDLDSLVKF
                     VKNKFPEGRLGVHGFSMGAGTAAMHSEINSKDDKVDFYILDSPYSEMKDAIRMGVLEK
                     RIPDILINYVVTCGDLYNKFKSGFWYSDVKPYESVEKSNVPILFIHGTKDTVCNYQNS
                     KKMYDLVKHDKKDLWLIEGIGHVDGFEHDSTVYFNKIFKFIDSNVLSDKS"
     gene            81940..82389
                     /locus_tag="KR250_000597"
     CDS             81940..82389
                     /locus_tag="KR250_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0066566.1"
                     /translation="MKNKNNKDKNMLNIKVLRVLGVLLILFDYTGDTLNFYNFFAKPL
                     VSGNLYKTTGTDCIVFLLFIVECVYLVGRYILKVKINWSEIIENEKGNFINNLLVEKI
                     QEESNIVYNLLNSYVLPNIYKKARGLMFKTYHMLIYIKEKYYYVNIY"
     gene            complement(82550..83479)
                     /locus_tag="KR250_000598"
     CDS             complement(82550..83479)
                     /locus_tag="KR250_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0066567.1"
                     /translation="MDSLSSLTPVEVAELLKITKNTVYELIKRGELPSYKVGKKIRVD
                     TKDVEEYINSQKTGKVKDTHFNNKNIDINNNFKIIISGQDVILDILARSVEKKLDGIS
                     TFRSYIGSYNALYELYNGRVSIASSHLWDFETDEYNSTFVKKLLPGIPCVLINLAYRM
                     QGFYVAKGNPKEITTWEDLTKPSITIVNREKGSGTRVLLDGKLRLLNFNGKHIKGYDN
                     EELSHLGVASTVSRGIADVGLGNEKAALQVNNIDFIPLHKERYDLVIKKEDLQNPVYQ
                     TIINIINSPEFKAELQGLGGYDLTDTGKTIAKV"
     gene            83672..84484
                     /gene="modA"
                     /locus_tag="KR250_000599"
     CDS             83672..84484
                     /gene="modA"
                     /locus_tag="KR250_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBH0066568.1"
                     /translation="MKKILGILGLVACLTLGTVGCNSNEDKKDDGKQEKTTKSSDSVE
                     LNISAAASLKEAMAKIEEEYKKVDSNVKLTVNYGASGSLQQQIEQGAPCDLFISAGQK
                     QMKALDEEKLLVSDTMKDLVKNDLVLISSADSSVSGMEDLTTDKVKKIAVGEAESVPA
                     GKYADEVLTNLNLKDKLKDKLVFAKDVKEVLAWVQSGNADVGFVYFSDTVNNDKIKVV
                     EKTDEKTHSPITYPVSVIKASKNVDAAKKFEEFLLSESGQKIFEEFGYKKVE"
     gene            84633..85316
                     /gene="modB"
                     /locus_tag="KR250_000600"
     CDS             84633..85316
                     /gene="modB"
                     /locus_tag="KR250_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HBH0066569.1"
                     /translation="MGTDWSPLWISLKTSILSTIITFVIGIFVSYIMANYRGKWKGLI
                     DGLFTLPLILPPTVVGFFLLLLCGKNGFIGKFLLNFDRTLIFSWTATVISAVVVSFPM
                     MYRTSRSAFEQIDNNMLSAARTLGLNEWKVFFKIAVPLAWPGIIGGLVLSFARALGEF
                     GATLMIAGNIPGKTQTMPIAIFFAVEGGDMNKAMMWVMIIVAISIAMILLLNYWSEFQ
                     QKIIGKRCG"
     gene            85317..86393
                     /locus_tag="KR250_000601"
     CDS             85317..86393
                     /locus_tag="KR250_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HBH0066570.1"
                     /translation="MSLYVDIEKDLSSFKLKVEIKQEKGTLGFLGESGSGKSMTLKCI
                     AGLEKPTRGKIVLNDRVLFDSEKKINLSTQDRKVGFLFQNYALFPHMTVSQNIELGLL
                     KLSKSEKKEIVARYLDILKLNGFEGRYPWQLSGGQQQRVALARALATSPDILLLDEPF
                     SALDHHLRSNMEKELMNMLKDYKGDILFVTHDIEEAYRVCDDIIVYNKGEGLPKRPKK
                     ELFESPKYLIEAKITGCKNISKLNRLDKNTIYATDWGCELTLNREIGDNIEYVGIREH
                     HIKVLDSNEDLNEKLCFELINIVENPFTYTIYVRKTDLSNECVPIQIELEKSKMRFKK
                     GDRIYLDFPQEYLFCFRYNYNKKE"
     gene            86399..86956
                     /locus_tag="KR250_000602"
     CDS             86399..86956
                     /locus_tag="KR250_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HBH0066571.1"
                     /translation="MTEKEKMLSGKGYYANDELLVKEREYCKKLTRLFNNTLEDEYEK
                     REDILRQLFGSVGKQINVEQNIRCDYGYNIHVGENFFANYDCIFLDVCKIEIGDNVML
                     APNVQIYTAYHPIDAQLRNSGIEYGSPVKIGDNVWIGGGVIITPGITIGDNVVIGAGS
                     VVTKDIPPNTVAVGNPCRVIKKIEE"
     gene            complement(87120..88142)
                     /locus_tag="KR250_000603"
     CDS             complement(87120..88142)
                     /locus_tag="KR250_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBH0066572.1"
                     /translation="MINKKRLASLILAGALSISMLTGCSQGGDSGNSKQESNSKGKEV
                     KKIGITQLVEHPALDATRTGFVKALEKNGFKDGENIDIDFQNAQNDMPTTQSIASKFA
                     SDKKDLIFAISTPSAQAAFNATKDIPILITAVSDPVAAGLVKTLEKPGTNVSGTSDFV
                     SVDKGLELLKIFAPKAKTIGVMYNTSEVNSKVQVDALKEYASKNGFKVVEKGITTSNE
                     VNQGISSLVGKIDVLYVPTDNLVASSMPIVSKIATENKIPVIAAESGPVEKGALACQG
                     INYEKLGYKTGEMAVKILNGESVSDMPVATSDDTDIIVNEDILKALGMEKPSNENISY
                     VKTKQE"
     gene            complement(88273..89028)
                     /locus_tag="KR250_000604"
     CDS             complement(88273..89028)
                     /locus_tag="KR250_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBH0066573.1"
                     /translation="MLQIKNLSKSFPNPYGEPNTIFENLSIDIEDGEFVSIIGSNGTG
                     KSTLLNIISGLIKESSGQVLLNKHNLSNLAEHRRTQIVSRVFQDPSLGTCPSMTVREN
                     LSLALNKGKLLNLRKCLRYKRDFLENLLEGVSLDLKKYLDVQVKFLSGGQRQSLSLIM
                     SCLTNPSVLLLDEHTAALDPKTSNEVIELTNKIVREKNITTLMVTHNLKHALHYGDRL
                     IMLHKGEVVLDVKGKEKEQLTVEEILEKFEYAV"
     gene            complement(89015..89917)
                     /locus_tag="KR250_000605"
     CDS             complement(89015..89917)
                     /locus_tag="KR250_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0066574.1"
                     /translation="MSGIISVMTQSLILSIMALGVYITYKILDFPDMSADGSYTMGAS
                     IVAFSLTNGISPVVATLMAILCGCTAGLVTGILHIKFKISNLLSGILVMGMLYSINLR
                     IMGKSNIPLFSFKHLFNGEISPIVLALAFVFICKVLLDLFLKTGLGYTLKGVGDNSQM
                     IKSLGINIGSIKILGLMISNGLIALSGSLMAQFLGFSDVNMGIGTLVLGIASIIIGIT
                     LFKKFTFIKDTTAIIVGSFIYQFTIYFAMSLGMLSTDLKLITAIVIISFLATGNLNIS
                     LKKNKCKASTKNKSEKRGVVDVTN"
     gene            complement(90726..91763)
                     /locus_tag="KR250_000606"
     CDS             complement(90726..91763)
                     /locus_tag="KR250_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBH0066575.1"
                     /translation="MIKSKKILSLIIAGVLGVSMLTGCSQNDGSNASNENKETDSKKQ
                     KNIGITQLVEHPSLDKAKKGFIKALEDKGYKDGDNIKIDFQNAQNDMPTTQSIASKFV
                     SDKKDLIYAISTPSAQAAYNATKDIPIIMTAVTDPVEAGLVKSLEKPGGNVSGTSDYL
                     SIDKTLELVKTLTPKAKKIGVIYNTSEVNSKIQVDSLHDYAKKNNYEVVEKGISSSSE
                     VNQAISSLVGKIDVLYVPTDNLIVSSMPIVSKVANENKIPIIASEEGSVSSGALACCG
                     IDYEKLGYKAGELAIEVLEGKSVGDIPVTTLDETEIIINEDTLKALDMQKLSADNIKY
                     IKSDENAKSTK"
     gene            complement(91860..92615)
                     /locus_tag="KR250_000607"
     CDS             complement(91860..92615)
                     /locus_tag="KR250_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003399531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBH0066576.1"
                     /translation="MLQIKNLSKSFPNPYGEPNTIFENLSIDIEDGEFVSIIGSNGTG
                     KSTLLNIISGLIKESSGQITLDSTTITNLAEYKRTQIVSRVFQDPSLGTCPSMTVREN
                     LSLALNKGKLLNLKKCLRHKTNDLEHLLEGISLDLKKYLDIKVQYLSGGQRQSLSLIM
                     SSLASPKVLLLDEHTAALDPKTSNEVIELTDKIVREKNITTLMVTHNLKHALQYGDRL
                     IMLHKGEVVLDVKDKEKKDLTVEEILEKFEYAV"
     gene            complement(92602..93504)
                     /locus_tag="KR250_000608"
     CDS             complement(92602..93504)
                     /locus_tag="KR250_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0066577.1"
                     /translation="MSGIISVMTQSLILSIMALGVYITYKILDFPDMSADGSYTMGAS
                     IVAFSLTNGISPVVATLMAILCGCTAGLVTGILHIKFKISNLLSGILVMGMLYSINLR
                     IMGKSNIPLFSFKHLFNGEISPIVLALAFVFICKVLLDLFLKTGLGYTLKGVGDNSQM
                     IKSLGINIGSIKILGLMISNGLIALSGSLMAQFLGFSDVNMGIGTLVLGIASIIIGIT
                     LFKKFTFIKDTTAIIVGSFIYQFTIYFAMSLGMLSTDLKLITAIVIIAFLATGNLNIS
                     LKKNKCKASTKNKSEKRGVVDVTN"
     gene            94303..95883
                     /locus_tag="KR250_000609"
     CDS             94303..95883
                     /locus_tag="KR250_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HBH0066578.1"
                     /translation="MLIGTSKLSRNVDTACVEEVGIPMIVLMENAVISAMKNMDIDMY
                     NSYTVVCGVGNNGGDGLGIARHLNTYGKEVNVFLVGNLEKLSECSKINYNVLLNMGIN
                     IININKNDLSEKNYGEENEVDKIIKVEKQNIIKNKNLTIFKDRVSKSDVVVDAIFGTG
                     LKRDITGIFKEVIDIVNDNSKNTYSIDIPSGIDSDNGNVLGTCIRANKTISFEFYKRG
                     FLNYDTSALIGDVVVEKIGVPDFITRKYHDEEFITDKTFVKNNIRRRDKHGFKSDFGK
                     VSIVAGSKGFYGASFIATESAVKSGSGLVTLISSEDVQEKVSIRLTEAMTLNFEEERL
                     NKLLNSSDAIGFGPGMGDNSQTFDKLLKIVENSNCPIVLDADGLNVMKDRCYKFLEWK
                     NRFVITPHLGEMARLTGDSIGYIREHRVDVAKEFAQKYNLVVLLKGYQTVITDGKKTY
                     INPTGNSCMATGGMGDCLLGMITSFIGQGMDILEATVSGAYIHGYIGDKLSKAMYTVN
                     ATDLISNISLTMKELLDI"
     gene            96061..96507
                     /locus_tag="KR250_000610"
     CDS             96061..96507
                     /locus_tag="KR250_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HBH0066579.1"
                     /translation="MKLIWLIVAVLFGIAEMLTPSLTLIWFSVGAVILIFLSSFIESI
                     FLQILIFAVISIAMLVVATKKIVKKDKGYKSNTNLQAMMSKKGIVTEEISPNNTGLVV
                     VEHETWTAISIDGEKIEKGSTVEVLKIEGVKLVVKKVDATVNVTNQ"
     gene            96528..97571
                     /locus_tag="KR250_000611"
     CDS             96528..97571
                     /locus_tag="KR250_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HBH0066580.1"
                     /translation="MGTKIVLSIVLIVVVVAISLTCIRVIKQSKVGIIMRLGKFQKVA
                     ETGVHFLIPFLDKMAYVIDLREIVIDFPPQPVITKDNVTMQIDTVVYYKVTDPVRYVF
                     EIANPIAAIENLTATTLRNIIGELDLDETLTSRDIINVKMRTILDEATDKWGIKVNRV
                     ELKNIMPPQDIQVAMEKQMRAERERREAILQAEGNKSAAILQAEGEKQSAILTAEAKK
                     EAMVRVAEGEKESAILVAEGEAEAIRQTAIAKAQGEAEMIKRTQMATAEGLKLVFSAM
                     KEADIDNNILALKSMEALEKMAEGKSTKLVLPSEAVNFLGTFKGIKEVMSDDNKEVLD
                     IKEVLNDNESLKK"
BASE COUNT        37391 a        11059 c        17545 g        31930 t
ORIGIN      
        1 ttaaaagtta tacaataaaa atagctgtgc aataatattt agactatgaa agtagttaaa
       61 aaatatttat gcacagctat ttataataga atttactttt tactagtgat tttaactaaa
      121 tcttgaaatt tactcataat gtaattgtaa ttatcttttt tatgaggtaa tatacaacta
      181 ctgcaatctt tgataccttt atctgtatac ttaaagtttc caccacagtt ttctcctaaa
      241 gcatacaaag gacaatagca aaatagacag ttaaactctt ctggtttatt agttttatga
      301 caagggaaaa attcgcaatc tttgtgatta aaaaacttat aattttcgcc catattttcc
      361 tcctaattaa ataaaattta aattttttat actatataag attttggtga taatataatt
      421 atactaaata attttatgaa tagtcgataa tatagtatta ttaatcatag tattaatggt
      481 ataattagtc aacagaaaaa taatgtaaaa ataatgtaag tgtattttgt aattagaata
      541 tgttttataa caaaaaatga ggtgattaag tgaaagaaat tcaagaaaaa gcacttcttg
      601 taggtctgaa tttgactaca atagtgaaaa aaaatgatga tatagacact aatgaatcta
      661 tggaagaatt aaaagaacta acaaaagctg ctggggcaga agttgtaggt agcttaattc
      721 aaaataaaca ctctgtagat gcagcttatt atataggaaa aggtaaagta gaagaaataa
      781 gagcctatag tgactcttta gatgctactt tagtaatttt taatgatgaa ttatctggtg
      841 ctcaaatcag aaatatagag aatgttgttg gaagaaaagt aatagataga actactttaa
      901 ttttagatat atttgctcaa agggcactta gcaaagaagg taaattacaa gttgaattag
      961 cacaattaaa gtatagattg ccacgacttt atggaatggg tggtgagatg agtagaacag
     1021 gtgcagggat tggtactaga ggtcctggag aacaaaaact agaaatagat aagagacata
     1081 tacttaataa agcagcagat ataagacgtg aacttaaaga agtaaaaaag aatagagaga
     1141 cacagagagt taagcgttta aagtcaaata taccgattgt agcacttgtt gggtatacaa
     1201 atgcaggaaa atctaccctg ttgaacgaat taataaagac acacaaggat tatgaacaag
     1261 aaaaagaagt ttttgtaaaa gatatgcttt ttgcaacttt agatgtaacc ttgagaaagg
     1321 ctcttttgcc gaataaaaaa gaatttttag tagtagatac tgttggattt gtaagtaaac
     1381 ttccccatga tttagtagaa gcgtttaagg caacattgga agaagtacag tatgcagatt
     1441 taatcttaca tgtaattgat gcaacaaata ctagttatga attgcaaaaa agtacgactg
     1501 aaggtgtatt aaaagaactt ggtgtaaatg ataaaaaaca tatacttgta tataataaag
     1561 tggacaagct agaattggat atatatccta aaagtcaaga agatattgtg tatatttctg
     1621 caaaacaagg cataaatatg gataagttat taaatatgat agaaattgct ttaatggaaa
     1681 atacttattc agtaagtttg atgttacctt atgagagagg tgatatattt agtagaataa
     1741 aagataaata taatgtggag aattttgagt atggggaaag tggtataacg ctagatgtag
     1801 atttagatga agaagatttt aatatctata gagagtacat actagaaaaa taggttgaat
     1861 atggatataa actgggaaaa tatagataat cttgaagatt attttataac ctatcttctt
     1921 tataaagaat ctaaaacagt atctcaaatt agtaaaatta gaaatatttc aagtactgaa
     1981 gcaagtgagc aacttataca agcaaagtta aaaataaagg aaatgcaaaa agatgatttt
     2041 gaggcatcta aggatatttt ggataaattt ctagaactag ataaaattaa gagacttgat
     2101 tttatggata gcttagatga cgaaaaaatg gtatacttta agagaaaggt tttcaaaaga
     2161 atccttgtag aaaaaaatgc tgaagattta attgttttaa tttgggcgac aggtgagcta
     2221 aaagatgata ggtttttgaa attgcttcat cagctcacaa accacagaca ctcagatatt
     2281 agaagaataa cttattcagc aatcagaaag attgaatcac caagtagtag agaagtactg
     2341 caaaaaggtc tctacgacaa aaatgcacag actaggcagt actgtgcaaa agctctttca
     2401 aaattgggag atgaaaacag tttaaagatt ttacagcagt tgaaagacaa gaataagaat
     2461 tttgaaaaag aatatgtgct tagagcttat gacaaagcca ttaagtcatt ggaaaatgca
     2521 aaaaatttga aaaagtaatt ttttattaaa gcaatttgtt tacttaatga tgtatttcta
     2581 aagtttaaca aggtaataaa taaattaatg ggaggggttt caaatgatag taagacgttg
     2641 tgcaggtgga gtagtgtttt atgctaataa agtacttatt gtaaaaaatg ataggggtga
     2701 atggacactt ccaaaaggaa agatactagg agggggactt ccatatgaga gtgctgttca
     2761 aagagttaaa gtagaaactg gcatagatgc taaaatgata gatgtggctg gcgatactat
     2821 gtatgaattt ttctcaagaa gtagacaaca agaagtttgt aatgcaataa tgtggtatgt
     2881 tatggaagct tgtaacactg attgtgtact tgctcctgaa tttcaagaag gtggatttta
     2941 taaagtaaag gatgctttag aaatgttatc acatcataaa gagcaagctt tagtagaagt
     3001 atcttataaa aagtttaagg aattaaagaa attagtttca gagggcgatg atgttccagc
     3061 aaattaattg tgaatttaga ggattctgag gaagggatag ccccttcctt ttttaattga
     3121 agcagtttta agcgtaaatt atatagtctc tttaaactat gatttatcaa tttagagatt
     3181 tagtagttta tatttaaaat cagtaagata tatttaaaat aaaaattaaa aagtatacaa
     3241 ttttattata taattgtctt atgatgttga ttaaagaatt attaaaagtc catttatttt
     3301 taaatagaat aattttataa atatatattt gatgtgtata ttagaaaatg attaaaatag
     3361 gaggaaatac aatgagcaat tacaggacat tacatgaatt tggaatggat gaaataacga
     3421 ttgaaaagtc tgtttttatt ggatatgcta agcctataaa gagtgaagaa gaagcattag
     3481 agtttataaa tgaaataaag aaaaagcata aagatgcaac tcacaatgtg tgggcttata
     3541 ctgtaggtaa aaatatgaat atacaaagat atagtgatga tggagaacca caaggaacag
     3601 ctgggatacc tacattagag gttataaaaa aagaagattt gagagatgta gcggttgttg
     3661 ttactaggta ttttggagga acaaaattag gtgcaggtgg tctagttaga gcttatacaa
     3721 aaggtgctaa actgggactt gaagcaggta agataattta taaggtaatg tatcaagaag
     3781 ttaaggttaa gatagattat actcaacttg gaaaagtgca aaatgagtta atgaatttgg
     3841 ggtattttat aaaagatact gtatatgagg ataatgttga aatagtagta tattcaagat
     3901 tagaagatgt agagaaatta tcagaaaaaa tgattgatat aacaagtgga acaggaaaaa
     3961 tagttttagg agaggaattt tatttgtctg aacaaaatgg agaaatttta atataatcta
     4021 aaaggttatt agaatagtta atacctcaaa tagccaatta gtatttttaa gtgaaaagcc
     4081 attaaattga tttaatctaa atcaatttaa tggcttttta tcggattttg cacagaatcc
     4141 tttttttact tcaaataaac cgactaaaag taaaaatatc ccaaatattt tttttaatat
     4201 atctgaagac agataattag ctaatagtga accaaataca gctcctaaaa ccccaaacac
     4261 agctataggt ataaccaatt taaaaattac atttttattt tttatatgta tagcaagtgc
     4321 tataagtgtc atagggatag atgaaagtaa atttacgctt tgtgctattt tagtgtctat
     4381 gccagcaagt aaaacgagtg caggtataag gattgttcct ccacccattc ccattcctcc
     4441 aataattcct gcaaaaaatc caattagagc aaataacatt aaaacaccat ccttaatgct
     4501 gcaattatca taattattcc aaaagaaatt ctaagaaact ttccagtaac cttatttagt
     4561 aatttagctc ctataatacc accaagaata cttccaactg caactttaat tgttaagtta
     4621 acatcataag tacctttaga tacatataaa acagaacttg ctgtcgttaa gaatattatt
     4681 atagaaagag cagttgcatg agatttatgt tcttctacct taacgatgtc atttaatatt
     4741 ggaactaaaa gagtacctcc tccagaacca aaaactccat ttataaatcc tgtaaaaact
     4801 ccaattatcg tattttttaa gttaaaatca aagtattttt tctttaaatc atcagttttt
     4861 ttcataaaaa tcaccttctg aaatagtttt accaaaatag gcaaattata tttatataaa
     4921 ataagattaa atataatctt tcatgtttgt ttaattttgc ataaaaaaaa acatattaaa
     4981 acaacatatt aatatatggt ataatatatt aaaacaaagt agcaaatttg agaggagata
     5041 acaatgtcaa atataaaaat acaaatagac aaaactgtag aatggcttat aaataaggtt
     5101 aatgaggcaa atgcaaaagg attaattgtt ggtgtatctg gaggaattga ttcggcagta
     5161 gttgctaatt taattaaaaa ggcatttcca gaaaactcta tgggagttat aatgagcata
     5221 aaaagtaatc ctcaagatag agaagatgca cttaaggtta tagagggatg tgatatagaa
     5281 taccttgatt tagaccttat agaaccacaa agtgctatat tagacatggt agtaggaaat
     5341 ttaaaagata aacatttata tagagaagaa tatttaaaga tgacagatgc aaacttaaga
     5401 gcaagagtaa gaatgagtac aatttataca attgctaaca atctaggata tctggttgtt
     5461 ggtacagaca atgctgcaga aatacacaca ggatatttta ctaaatttgg tgatggtgga
     5521 gttgatattt tacctattgc aaatttaact aaaggagagg tgtatgaatg ggcaaaagag
     5581 cttggtgttc atgaagattt aataaacaaa gctccttcag caggtctttg ggaaggtcaa
     5641 acagatgaag atgaaatggg aactacttac aatatgatag atgctgtgtt agaaggtaga
     5701 ttggatgaag ttcctaaaag agaccaagag ataattgaaa gacttcatag attaagtgaa
     5761 cacaagagaa aaacgcctgc acaacctcct aagttctaat ttactattta taagatttgt
     5821 gataaaattt acaagtgatg aaaattagaa taattaattg tacgtattaa ggagtggaaa
     5881 tatgggacgt ataggtaata taattaatag aaaaggtaaa caagatgctc aaagagcaaa
     5941 gatatttact aaacatgcta gagcaatagc agttgcagct aaggaaggtg gagcagaccc
     6001 agagtacaat gcagcgttaa aaacagctat agaaaaagct aaggcagata atatgccaaa
     6061 tgataatata gatagagcta tcgctaaagg tgctggagca ggtgctggag aagactatga
     6121 aactatagtt tatgaaggat atggacctgg tggagttgct gttatagtag aaactttaac
     6181 tgacaacaaa aacagaactg ctggtaacgt aagatactat tttgacaaaa acggaggaaa
     6241 cttaggaact agtggatgtg tgtcttttat gtttgataaa aaaggacaaa tattagttgg
     6301 tttaggagat ggagtttcag aagaagaatt gatggatgtt gcattagaag caggagctga
     6361 agatttcata acagaagaag atggttatga aataataact acaccagaag atttttctag
     6421 tgttcgtgat gagttaaaag ctaaaggata tgagtttata tctgctgatg tgaagatgat
     6481 acctcaaact acaactgtat tgactgaaga atctcattta aaaatgatga ataaattagt
     6541 tgatatgctt gaagaagatg atgatgttca agatatatat cataactggg aagtagaata
     6601 aatagtataa gcatattgaa atttataagt attgaatcaa ttttaacatg tgataaattt
     6661 tatagaataa gaaacactaa aatagacata cgcacctcta acctatattt gcataataat
     6721 atgtaagcat aggtaagggg tgtttttata tttataggtt aagaaattta aagtaaattt
     6781 tgagctggac tcgattgtct ataaataatt gatgggtata taacttgttg ctgaatacca
     6841 tttatacgcc cagttactgt atcaataatc atcctaagac tacgttctgc tgtctcttcc
     6901 atttttaaat ctataactgt caaaggagga atacttcttg ccattagtcg tgaaggtcca
     6961 ttacttgctg caaatatttg aatatcctct ccaattacaa ttcccgattc atgaaatgca
     7021 ttcatcatac caagagctac cacatcatga ttacaaaaaa ttactttttt tagtgaattt
     7081 tttgcaagca ttttttgagc aatatcatat ccactatcta ttttattaga acaataatat
     7141 atagaatctg atagatgtat tccatgttct ttacatgttt ttaaaaaaga tttacccctt
     7201 aaagtcatac atgtaaagga agttgggttg aatacaacag caatatcatt tttaccattt
     7261 tgaatagcat gtaaagctgc tatacgacca gtttcctcat tatcaataga tacacttgaa
     7321 aaaccaggta tttcacgatt tacaacaact acaggaattt taggtttatt ttttactaga
     7381 tattctatat catcagcatt gatacctgtt aaaacaattc catcataata attatttgca
     7441 aaaaagtatt catcatcaca tagatgacca actttgtaag gtcgtatgat gatatttatg
     7501 tttaaatgtt ctaaagaaat tatgtggttt attgcttgaa gtatattggt tgatgccaac
     7561 tcaaatccct catcctgcca ataaaaagct attttaatat tattatgttt aataggagta
     7621 gagcgtttat atccaagctt atcagctgaa tttaatataa gctcttgtgt atgtagcgga
     7681 atcctgcgtt catttccttt accacttaag acgtacgaga cagttgctga agaaagacca
     7741 cagtcttttg caatatcttt gattgttgcc atgataaaat cctccttaaa tagttgctta
     7801 aatttgctta taccaataat cttaacatat attcaactaa agaacaatat tctacaaggt
     7861 gcatatttta cagaattaga ataaataaat ttaaaaaaac ttcttactta tgttgacata
     7921 atttagttta taggattata atatgcttaa ataagcttat tattaactta aattaagcaa
     7981 agttaagcag gaacatgcaa tgctagcttg caatgtgact gtaaaggggg aaaaggtatg
     8041 agtaaggtaa atatatttga agttggtcct agagatggat ttcaaaacct gaaagagttt
     8101 ttagaaactg ataaaaagct taagattatt gatggtctta ttgcttcagg gattaaacaa
     8161 cttgaaatta gttcatttgt aagcccaaaa gcaatacctc agatgaaaga tgcaaaagaa
     8221 gtagcaactt attgtgtaga aaaatatcct gatgtgaaac tttatgcatt agttccaaat
     8281 ttatacggtg ctaaagttgc atgggagtgt ggtataagag atatatccta tgttatatct
     8341 ttgagtgaaa ctcataataa aaaaaatata aatcgtaccc atgaagagtc tattgatgag
     8401 cttaaaaaaa taatgaatac ttatccagac atgaatatat gtattggaat ggctactgta
     8461 tttggatgtc cttttgaagg tttaccaaaa attgaagatg ccataagatt tgctaaacgc
     8521 ctttgggaaa acggtatcag ggagatgaat ctagcagata caatagggat tgctgaccct
     8581 aagcaagtaa gagaaactat tagtgcatta gttgaagctt taccagagtg cagatggcag
     8641 gtacatattc atgatactag aaatatggga atggttaata ctcttgtggc tattgaatct
     8701 ggaattacta ctgttcagtc tacacttgga ggattaggag gatgcccatt tgctcctgga
     8761 gcatctggca atactgcaac tgaagattta gtatatatgc ttgaacgtat ggggtatgat
     8821 acaggagttg attttgttaa acttcttgaa ttatcaaagt atcagaagag ttttgtaaat
     8881 ggagtatata gtggtcatca tgtaaatata aagtctgata gttgtaattg ttagttctat
     8941 tgattaatcg attaaaaaaa tatgttgatt aaaaaaatat gctgattggt aaaatagatt
     9001 tattgataaa aaatataaat tataaaatac attatctaat aaaacaaata aaaaaattga
     9061 gggggattag tatggatatt atagctttac ttttattttt aggagttatt attattgcat
     9121 ttgtacgtaa gaataatgca ggtattttgg ctctagctat tggtgtcata gctgttagaa
     9181 tttttggaat ggatgataaa gaactttatg gagctataag caactcaatg tttactacac
     9241 ttgttggtat aacactactc tttgcaatag tgaactcaac aggagcatta aagcttgttg
     9301 cagaaaaaat tgtagctgct tctggtaaac gtgtttggct tatacctata gcagttttta
     9361 tagcaggatt tgttgttgct ggtgttggac ctggggcaat tccagcactt gcaattatcc
     9421 cagcaatggc tgttcctgta gccataacgg ttggttataa tcctgtaatg cttgcactta
     9481 taggtgaatg tggattaatg gcaggtcgta tgactcctat tactccagaa ggtcaaatta
     9541 taacaaccgt tgcagaaggg gttggaattg aaaatgttat gcctactatc ttagcctgtc
     9601 aaactctttc tacaattgta ttttcaatag tattgtttat aattttcaaa ggatataaac
     9661 ttaaaaaacc tataaatgtt atagatagta agcaaataga aaaattcaat ataaagcaga
     9721 taatttctct aataagcata gtagttatga ttgcttttat cataattttt gatatgaatg
     9781 ttgggcttgc agcttttgca gtttcagctg tacttatttt tttcaatatt gctgatgaag
     9841 gagaatgtat caaatcaatt ccatggagca cgatagtaat ggttcttgga gtaggtgcaa
     9901 tgatgacaat cgtagatgca gcaggaggta ttgacttgat gagtaatgca ttatcatcac
     9961 tgatgaatag taaaacagct actccaatta tgagtatttc agcaggactt atgtctatgg
    10021 ttagttctgc tttagcagta gtttatccaa ctatgatgcc tatgtgtgtt gatatagcta
    10081 aggcagttgg aggagtagac ccactagcac ttattgctgc agttggtgtt ggaggttctc
    10141 ttgctggtgt aagcccactt tctactggtg gggcattgat tttagcagct atgggttcaa
    10201 gtaagaaaga ctttaataag gaagaacaaa ataaggtttt tgtacagctt ttcattatga
    10261 gtgcagttgg cttattggtt attgcagtag tatcaatatt atcatttaat gcaataacac
    10321 atttaatgaa ctaattagaa taactgaaaa taaatcatta aatttaactt ttaatattaa
    10381 tcaatataat ataatttgga ggggaagtta tgaaaaaagg agcattaagc aatataaagg
    10441 tacttgacct tacaagagtt cttgcaggtc catattgtac aatgatgttg gcggattttg
    10501 gagctgatgt tataaaaata gagatgccaa gaaaaggtga tgatacaaga ggttttggac
    10561 cttttgtaaa tggagagagt ttatactatg caaatataaa tagaaataaa aaaggtatca
    10621 ctctaaacct taaatcagaa gaaggaaaaa atatattttt agaaatggta aaacatgctg
    10681 atgttgtagt agaaaattac agaccaggtg taatggaaaa gttaggtctg ggttatgatg
    10741 agttggctaa agttaacaat caaataattt atggagctgt atcaggattt ggatgttatg
    10801 gaccatattc agaacgtcca ggttatgata ttattgcaca ggcaatgggt ggtcttatga
    10861 gtataacagg gcaagaagga ggagaaccaa cgagagttgg taatgctatg ggtgatgtac
    10921 taggtggaat gaatttaact attggtatac tcacagcact caacgctaga aaaataatag
    10981 gaaaaggaca aagagtagat gtttccctcg ttgattctgt tgtatcgtct ctcgaaactg
    11041 gtatacagag atactttgat tcaggacaac ctgaacttat gggtaataga tatgcttcag
    11101 cctatcctta tgactctttt aaggcaagtg atggaagttt tgtaataggc tgtggtaatg
    11161 acaaactttt tgaaaagtta tgtaaaaaag ttctttttag agaagacctt ttaatagatg
    11221 agagatttaa aacaaatctt aatcgttgtg aacattacaa ggctcttaaa ccagaaatag
    11281 aaaaatggtc tattaagcat acaataaaag aagcagttga cttaatatca gaggctggag
    11341 ttcctgctgc tcctatctat aatcttgatt gtgttacaac agatgaacat atagcaaaag
    11401 caagagaaat gattgtagat attagtcatc ctattatagg aaatatgaaa gtcaatggaa
    11461 atccagttaa gttgatgaca acaaaagcta aaattgatat gccagcacca tctctagggg
    11521 aaagtaacga ggagatttat aatggttggc ttggttttga tagtgataaa ataaaagatt
    11581 taaaagaaaa tggagttatt tgattaaaaa atagctaaac acacttgtga ctttagtcag
    11641 gggaggttca gttagaataa tatcaaaaaa tattaaatca atactgtaaa tcaaatatag
    11701 aattgtcaga agcatttaag atgggaaaag ctacttagac attgagtctt tgtggctttt
    11761 cctattttca ttaagaattt tatattatca gaatagatat gtactaaact aaagtattaa
    11821 caatttaaat taatagaaaa taaactacct aattatgttg aataaagact actttagtga
    11881 aaataaactt atagagatgc tatttaaatt attttaatgg aaatatcaaa aatgagactg
    11941 cattctcaaa aatgagactg cgttttttaa gtattttctc gaaaatgaga caaaaatatt
    12001 gttttatggg ttttattgaa gaaaaattct tctgtgaatt acaatttaat caattttaaa
    12061 gttgaaaatt attaaaaatt tagtgaaatt tatgtatatt tcgtttattt tattaaaaat
    12121 aacaattttt tactaaaaat aaagaacttc aatggtgatt ggcatagtac ttgctatttt
    12181 atattaacaa gaaatgtaga aaaaataaca atgtataatg gggggaatat gatggaatct
    12241 gtaatggaaa atttgaacaa tcttaaggtg gaggttaagg acaaagtctg tgttattact
    12301 ataaatagac ctaaagcttt aaatgctctt aatagtgaca ctcttaggga actatcacaa
    12361 gttattgatg tagtatcaga aaatgaggca atattaggag ttattattac tggagaaggt
    12421 aaagtttttg tagccggtgc tgatatacgc cagatgcaaa actataaaag tgaggaaggc
    12481 agaaaatatg caggttatgc tcagggagtt tttgataaaa ttgaagctct tgaaaaaaca
    12541 gtaatagcag cagttaatgg atatgcatta ggtggtggat gtgaacttgc tatgagttgt
    12601 gatattagaa ttgcatcaga aaaggctata tttggacaac ctgaagttaa tttaggagtt
    12661 attccatgtt ttggtggaac tcaaagatta tcaagacttg ttggaactgg aatagctaaa
    12721 gagttaattt ttacagggag acaagtaaat gcagaagaag ctaaatctat tgggcttatt
    12781 aataaagtgg ttccaagtga tttactttta gaagagtcta tgaagatgat gaaccaaata
    12841 gttgaaaaag cgccaatagc aataagatat gcaaaagttg ttattaacaa agggatagat
    12901 atggatttaa agaatgctct tgaactagag aaagatattg caggtttaac ttttgctaca
    12961 agagataagc aagaaggaat gaatgcattt atagaaaaga gaaagccagt ttttgaaaat
    13021 aagtagctat tataactaaa attggaacaa tgtagaatat attcaaaatc taaatttcgt
    13081 gaaaaaatac atatagttta attttaaaag gagttaattt atgaatacgg gagtaattat
    13141 aagtttagta gggattatca tagctctatc tcttctagtt gtaatggtta tgaagggaat
    13201 aaatattttt attatagcaa ttgtatgttc atcattagtt gctataacag gaagcctaaa
    13261 tgtgtatgat gcacttaaag tggattatat gacagggttt gttggatttc ttcaggcaaa
    13321 tttctttatt ttcctaacag ggacattaat gggaaaaata atggagatta ctggtggagc
    13381 aaaatctatt gctaagatga ttgtaagatg gattgggaag gataaagcac tactttctat
    13441 tccaatagct tgtggaatat tagcctatgg tggagtaagt gttttcgttg taagttttgc
    13501 agtgtttccg atagctttag aaatttttaa agaagctgat ttgccacgaa gatttatacc
    13561 agcagctttg acttttggat gctcaacttt tgctatggtt gcaccagggg caccacaaat
    13621 tcagaatatt gttccagcat caacattagg gacagatatt atggctggag cagtaaatgg
    13681 ttttatatca tgtgcagtaa tgtttatagt tggaagtaca attttgtaca aaatggtttc
    13741 aaaagaaaaa gccaatggtg gtcattttat agcacatgaa tcagatatat ttgatgaagc
    13801 agaagcaact aattctaaga gagaaaatgg tcccaatggt gtaattgcct taattccatt
    13861 gatagtgtca attttaataa ttaatgtaaa agttaatgga aaagcgatag taccaattga
    13921 ggtgggagta tttattggag ctttattagt atatttattg ttaaataaat atcaagataa
    13981 tactaaaata gttggacatg ttggtgatgc atgtaaaact acagtagtag caatatgtaa
    14041 tacttgtgct gtagttgcat ttggttcagt agtaaaatca gcagttggtt ttgacttcat
    14101 agttaatgct atgactaata ttcctggtcc tcctattgca ggggctgcag ttggaactac
    14161 agttattgca ggtatttgtg ggtcagcttc aggtggacta ggaattgcag ctccacttct
    14221 tggaccagta tttttagcca agggtgtttc cgctgcagca ttaactagga ctatggcaat
    14281 ttcatcagca gcattagatt cacttccaca caatggatat attgttactg taacaaatgg
    14341 tctttgtaaa gaaacacata aggatgccta tatgccaata ttctgggtaa ctgttttaac
    14401 cccatttatt ggaaccatag tagcagtcct actgtttaca atgttcccgg gattgccata
    14461 agagaaaaga taaaaggtat aaattatatt ttgatgttat aaaatcaatt ttcaaaatat
    14521 atacaataaa tttcatgttt taatactgta aatatgtatt taaaataaca aattgaccat
    14581 ataaggagga attgaaagtg ttaaataaac cattagaggg agttaaggtt attgacctaa
    14641 cgtattttgt agcaggtcca gggacttcta aaatattagc tgactggggt gctgacgtta
    14701 ttaaggtaga gccaagcttt ggagacccag gaagaaagac aggggcaaca atgacaatgc
    14761 caatagatga ttataataat cctttttaca gcacatataa ttcaaataaa agaggtttgt
    14821 ctataaatct taagagtgaa actggaattg aaattatgga taagctttta agcgaagcaa
    14881 acgtatttgt ttcaagttat agaacaggag cattaaaaag actaggtctt gattatgaat
    14941 cattaagtaa aaagcatcca caccttatct ggggtcagat aaatggtttt ggcgattttg
    15001 gacctgctaa ggataatgca ggatttgata cagttgcctt ttgggcaaga agtggggcta
    15061 tgcttgatat agcagaaaaa gatacaagtc caataaatcc acctatagcc tttggagatg
    15121 caacaacttc ttgtagttta tctggaggaa tatgtgcagc tttgtaccag caagctaaga
    15181 ctggaaaagg acaaaaagta atggcatctt tatttgggca agcaatatgg aatgcatcat
    15241 ctttattagc atctacacag tttagtgatg agtatccaaa aactagaaaa aatgctattt
    15301 caccagtaat aaattcttat cgttgtaagg atggaaagtg gatattctta agtattttag
    15361 agcatgaaag atattttgag gcactttgta gattatttgg aagagaagac ttaataaaag
    15421 aagagaaatt tgcatcttca gtagaaagta agaaaaatgc tacagaactt attaatattt
    15481 tagatggtga atttgcaaag tttactcaaa atgagatggt tgaaaaactt actgctgctg
    15541 atatagctca tgaaaaaatt caacatgtta atgatgtagt gactgatgaa caggctattg
    15601 ctaataacta tatatatgaa catacaagta aagatggaaa taagacaatg cttgcatcta
    15661 ctccagttaa atttggaaat attgaagtta acatgacttg tgatgctcca cttataggag
    15721 aacattcaga tgagatattg agagaacttg gatacagtga ttctgatata gaggctctaa
    15781 ttgaatctgg aattgtaaca atgaataaag aagcatgtga aatatgttaa taaacatgtg
    15841 aagtatgtta ataaatagga gggaatttta tggattttag attaacagaa gcacaattaa
    15901 tgcttcaaag agtagcaaaa gaatttgcag agaatgaaat agcaccaata gctgctgaaa
    15961 cagataaaac aggtattttt ccaagggagc tttttagcaa aatggctaaa ataggattta
    16021 atggaattgg aacacctgta gagtatagtg gttctggtgg ggcagatata gaaaaggtaa
    16081 ttgttgttac tgagattgcc aaaaaatgtg cagcatctgc agcaatatta tcaatacaca
    16141 caatatatgc tcaagctatt ttgaaatttg gtactgagga acaaaagaaa aaataccttc
    16201 caatgatggc tgaaggtggg tgtgtaggtg cttttgcctt aaccgaacct aatgctggtt
    16261 cagatgctgc gagagctgca acaactgcaa ttattgatga agaaactgat gaatatgttt
    16321 taaatggaac taagtgtttt atttctggtg gaggtcaagc agaatcatta ataatatttg
    16381 cattgacaga cccatcaaag ggaataaagg gaatgtctgc tatcattgtc gacaaaggta
    16441 cgccaggttt ttcaatagga aaaattgaag aaaaaatggg tattcatggt tcagaaactg
    16501 cagagttaat atttgataac tgtagagttc caaagtctaa cttattagga aaagaaggta
    16561 aaggatttaa tatagctatg acttgtttag atggagcaag aattggagtt ggagctcaag
    16621 ctgtaggaat tgctgaaggg gctttagaag aaagtatcaa atattctaaa gaaagagtcc
    16681 agtttggcaa gccaatttca gctttgcaag gtattcaatg gtatatagca gatatggcga
    16741 caatggtgga atcagcaaaa ttattagtat attatgctgc agatttgaaa gctagaggag
    16801 aaaaacacac taaagaagct gctatggcaa aatacaatgc gtcacgtact gcaagagagg
    16861 ttacaaattt agcccttcaa atccatggtg gatatggata tatgaaggat tatccattag
    16921 agagaatgta cagagatgcc aaaataacag aaatttacga aggtacttca gaaatacaca
    16981 aagttgttat ttcaagagca gtactaggat aggaggaaaa gaatgaatat agttgtttgc
    17041 ttaaagcaag ttccagatac taatgaagtt aaaataaata aagagacagg tactctaata
    17101 agagatggtg taccaagtat aatcaatcca gatgatagaa atgctttaga agaagctcta
    17161 aaaatgaaag atgaactagg agcagtgatt aaggttataa gcatgggacc tcctcaagct
    17221 aaaagtgctt taaaagaagc tttggcaatg ggagctgatg aagcttattt aatctcagat
    17281 agagcttttg gtggttcaga tacttgggct acatctacaa ttattgctgc tgctatagag
    17341 aaggttggaa agtatgatgt aatattctgc ggaagacaag ctatagatgg agatacagct
    17401 caggttggac cagaggttgc agaattttta gggataccac aagttactta tgcgaaagaa
    17461 gtaaaggttc aagatgacaa attactagta actcgttata cagagacagg ggattattta
    17521 atagaagcaa agatgccagt tttattaact gctataaaag agttaaacaa tccaagatat
    17581 ccaagtgtca agggaatctt agaagcttat aataatggag atgcaaagat tactgtatta
    17641 acacttgcag atttagatgt tgatacaact caaattggat taaagggttc acctacaaac
    17701 gtttataagt cttttgtacc agttaaagac aagcataatg aaataataga aggtataaat
    17761 aaaaaagaaa aagctgagaa actaatagaa atactattcg atttgaaact agtataggag
    17821 tgttaagatg atgagagcaa aagttaatca aggtataaat ttaaatgatt ataatggtgt
    17881 ttgggtaata ggagagcaaa gggaaggtaa aattaatcct gttacgatag aacttattgg
    17941 tgaaggaaga aagcttgctg accaattagg aaaagagctt gcagttgtga tagctggata
    18001 tgaagtagag aaagaagtga aggaattact tcactatagt gttgataaag tatattacat
    18061 caatgaccct cttcttaaag atttcactac tgatggatat gcaatttcaa ttgcaaattt
    18121 aatcgaaaga aaaaagccag aggttgtatt ggttggtgct acttctatag gaagagatat
    18181 agcgcctaga attgcaggta aggttggaac aggtctaact gctgactgta caaaacttga
    18241 aatagattca acagataata agttattaca aacaagacca gcatttggtg gaaatttaat
    18301 ggccactatt gtttgtccaa aaaatagacc acagatgtca acagttcgtc caggagttat
    18361 ggcaaaagct gtaagaaacg aatcagagac tggaatttta gaagttgtta ctccagaact
    18421 tactgaaaaa atgattagaa ctagattagt agagatactt ccacaagaaa agaaatctgt
    18481 aaatctaact gatgctagaa taatagtatc aggaggaaga ggattaaaaa gagcagaagg
    18541 atttgagctt ataaaagaat tggctgataa attgggggca gagattggtg cttcaagagc
    18601 agcagtagat tcagggtgga tagaacattc tcatcaagtg ggacaaacag gaactactgt
    18661 tagaccagaa ctatacattg catgtggaat atctggagca atacaacatt tagcaggtat
    18721 gagtgattct aaatatatag ttgctataaa taaagatgca aaagccccta tttttagtat
    18781 atgtgactat ggaatagtag gagatttata tgaaattata cctgaaatga tagaatcatt
    18841 aaataggtaa cttttagaaa acattgaata tgtagaatga taagtcttat aatattgtat
    18901 tgtaagactt attatttttg ctgaaggtta tagtggggag atgaaacaat gaaagagcag
    18961 tggtataagg atatatttgc aagagtatta tctatgactg atgatggttt tattgttgta
    19021 aatagaagtg gagtaataat agatattaat gataaatatt gtgacttttt aggaaaagaa
    19081 agaaaagata taataggaca aaatatacaa agtattatac caaatacaaa aatgctagat
    19141 gttatgaaaa ataagtattg tgaagaagga gctatacatc attattcagg tggaaataca
    19201 aaagaaaaaa gtgtaatagt aagtaggtct tatgtggaaa atgacaatgg agaagtagtt
    19261 gcaggagtag ctcaagttaa atttagattg caatcatttg atgtagctaa aaagttaatg
    19321 tcagaatata tggagctcca atattataaa gagcagttta aggataactg tggttttgac
    19381 aagctcatag gtgaaaatag agacttcata gaacttaaga aaacaggtgt gaaagcttct
    19441 aaaacaaact ttccagtact tttaacgggt gaaacaggta caggaaaaga agtctttgct
    19501 agagcaattc acaataatag cagtaggtca gataaaccta tggttagtat aaactgcgct
    19561 gcaataccag aagaattatt agagtcagag ctatttggct atgacgaagg agcttttaca
    19621 ggagcaaaaa agggtggtaa aaaaggtaaa tttttagttg cgaataatgg tacaatcttt
    19681 ttagacgaga taggtgacat gcctttgacc atgcaagcaa aacttttaag agttcttcag
    19741 gaaagtgaga tagaaccagt gggaggtctt aaaactataa agatagatgt aagagtaatc
    19801 tctgccacaa gaaagaattt aagtaaaatg gttgaagagg gattatttag agaagaccta
    19861 tactatagat taaatgtaat aaacattcat atgatggaat tgaaggacag gcaagatgat
    19921 attttattgc tagcaaatta tatattaaac aaattaaatg ttgaatacaa ggaattgaag
    19981 gttttaagtg ataaggttaa aaattgtttt attaactata cttggccggg aaatataaga
    20041 gaactacaaa atgtaataaa aagtgcctat gctgtaagtg atgatatggt cattatgatg
    20101 tgtgaccttc catctaagat ggataatatc agcagagtag ctcaatgtaa tgtggatagc
    20161 aattgttcaa ttcatgaaat ggttgagaat tatgaaaaga gcttaattat tgatgtgtta
    20221 aggaagtata attggaaatg ttctaaggca gcagaggtta tgggaataca caagagtctt
    20281 ttatataaga aaataaaaaa gtatgaaatt gaattaaata attaagataa attgaattaa
    20341 ggcaatgcta aaaaaacgcc cttattcaaa gcgaactgta taaactgttt tatgcagtta
    20401 aaacgtgtaa aagtagatgt gaagtactaa tgtcaatcat tagtactttt ttattatatt
    20461 tataattact atctatattt cagtatcttt tgatatctaa gattattatg tttaatttag
    20521 gatataacaa aaccctctta gttatggaca gttttttata ttttaactgt ctactaagag
    20581 gggatagttt agtttaaatt tagagtaaat tttatctagc atttataaaa aatattttga
    20641 atagcttttt tgttatttaa tcataatttt ctacttcttt atcaagttct ttgccacaag
    20701 aatcacactt atatatagca ttgtatccgt aatcatattc atcaactatt ttcttttgtc
    20761 taaatacacc accacactca cataggaaac caatttttat aagtgtaacg ataaattgtg
    20821 attttataaa ttttatatca ttacatttat cgttagtgta aaatgtataa atataagagt
    20881 tatcgcactc atctcttaat tcaaatcttt caatatatgt gttatttttt aattcctcaa
    20941 attcttcttt agttaaatat gatttttgtt gcaattcatg taaacgctta atttggtcca
    21001 ttatttatat cctcccaata attttagtat tttaagctta gataaggaat ctaagtttta
    21061 ttaaatttat cccaaggaat tatttaaatc atatttagtt ttattaatta caacatatat
    21121 aaattacacg aaaataaaac taattgtaaa aataatatcc tgtgaaaacc taaactacta
    21181 accccttact aacccgaaaa ctaaaaaaag aaaatatatc caaatataag tgatatataa
    21241 ataaagatgt ttcatataaa tttaacttta aatattctga caataatata tataaatata
    21301 ttatagttaa aatataccac atattttggg tattgtatac tggaattaga gtaattaatt
    21361 tattttttaa catacaaatg attttatgaa aaaaaacaag actcaaaaat ttttatattc
    21421 tttgatagaa taaaattgag agtaaattta ttgaatttta taaaatttat aaggggtgag
    21481 tctaagatat gcatgaagat aaaaatgata tatttgaatc aatagatact gaggattttg
    21541 ataataaaac agaagaagaa tgtttagagc tagtaagaaa aaatggattg aatttaatat
    21601 acataaaaaa tcaaacagaa gagatatgtc tagaggctgt tagacaaaat tgtaatgcaa
    21661 taaaatgtgt tcagaatcag acagaaaaaa tatgtataga agcagttaaa caagattgga
    21721 gaatgttaga atttgtagaa gagccaacag aagaaatttg catgttggct attaaccaag
    21781 atggaacagc attaaaatat atagaaaatc agacagagga gttatgctta agagccgttg
    21841 aaaaaaatgg tgcagctttg gagtatgtaa aagaacaaac agaagaaata tgtatagaag
    21901 cagttagaaa cagcgaattt ggattggcaa gagtgaaaaa tcaaacagaa aaaatatgta
    21961 tggaagcagt taagcattgt agctataatc taaaatgggt agaaaatcaa acagaagaaa
    22021 tatgtatgga agcagtgaga caaaatgggc ttgatttgaa atttgtaaaa aatcaaacag
    22081 aaacaatctg tttaagagca gtaagacaaa atggaatggc attagaattt gtaaaggaac
    22141 agactgttgg tatatgctta aaggcagtaa gacaatatgg aatggcatta aaatttgtaa
    22201 aagaacaaac agaagaaatt tgtacagaag ctataaaaca agataaaaga gcacttagtt
    22261 ttgtaaaagg agataaggaa aaatacaagg ctctatatga taataatgaa ccatttgcaa
    22321 agagatatgt tagaaatgta atagaaaaag aaaacagagc attgattaaa aaaggagaag
    22381 aaaatgcaaa aatcaaaatt gcaggaaaac tttatgatag aggcatgtct tttgaagata
    22441 tatcagatat agttgagatt gaaataagca agttaaaagt gagtttaggt gttttttaag
    22501 ggaaatagtt aataaaaaaa taatcgacta gctctttgta ggacagcaaa attagtgata
    22561 taatttgatg tataataaat tatttttatt agataagggg gaattaaagt tgaagattac
    22621 agtattatac caaacaagaa gtggaaacac agaaagggtc gctaagctga tagaagaggg
    22681 cgctaaaaag gtggatggag tagatgtaaa gcttatgaga cttgataata ttgatttaga
    22741 ttatttaaat gaaagtaaag cagtaatatt tggaacacct acttatttag caaatacaac
    22801 ttgggaagta aaaaaatggt ttgatgaaga ttcaaaaaaa gttaatttag ctggaaaatt
    22861 aggagctgtt tttgctacat gtgattatat atgtggaggt ccagatgttg cgatacttac
    22921 tatagtagga catttgatgg ttaaagggat gcttgtgtat tctggaggtg gttctttagg
    22981 aaagccattt atacatttag gacatgtaca ttcaattgaa ggtccagagt tacaagatga
    23041 gaaggctata atatttggtg aaagaattgc tactaaggca aaagaactat ttgcttaatt
    23101 tctatatgca taataatgat tagagtaaat ttagggtttt aatggagatt tgaagtaaaa
    23161 gttataaaat aaaaaatttt ccataaaatt actcaagatg aattataata taattataaa
    23221 taaattatat tattaaaata tttaaaatca aacgtcaata gcgagggaag tttatggtat
    23281 ggcatataga atatgagatt ttttctgcaa ttatagttat ttttttaatg gtatactttt
    23341 ttagaagtaa atttatacct actttacaaa acaaaatata ttgtgcactt ttaatattct
    23401 catttttatt tataatttca aatattttag gttcattttg tctaaataat atagacaaaa
    23461 ttccaatttt tattactttt ttattgaatc agatatactt attgttattg ccaataccag
    23521 cagcacttgt atcattttat gttatggcta ttatttatca agatataagg tatatgaaaa
    23581 aaagattatt atttttatct ataccactta taatttgtat aatcttatct attacaaatc
    23641 cttttacgca tattctattt ggattgtcaa aagaaacagg ttatatacga ggttgtggat
    23701 atgttgcaac ttttgcatct tttttctttt atgcaatata ttcagcattt ttatctttta
    23761 aatatagaaa agcaatgcat gaatcaaaaa tatgggctat tagaggtttt cttatagttt
    23821 caattgtggc aattatctta caggcaattt ttttacagta tcttcttaca ggaactgcat
    23881 gtgcatgtag tttacttttg acatatcttt ctatgcagaa tcgaggtctt attattgatg
    23941 accttactgg tgtactcaat agacaatctt ttattcaaga gctagatttg aatatcaata
    24001 ctgaacaatc aggatttatt attacaattg cgcttgatga ttttaagttt atgaatgaaa
    24061 cttttggaac aaaaaatgga gatattgcac taaaagaagt tggaaaatat ttgattcaga
    24121 tacatgatgt aaaccatgta tatagaatag ggggagatat attttctatt gttgtcaata
    24181 gtcaattagg tatagaacca gatgatatta tcagaaagat agaagaaaga tttaaaaagc
    24241 cttggctagt agaagagata agttttaatt tagcaactag tattgctgtt gtttattacc
    24301 cagaaaatgc agaaacgaca gaagatgtaa ttgttgctat agatttctct atacatgaag
    24361 caaaacgttc tgagtcaagg cgagttgttt atgcagatgc gtctataagt gaaaagataa
    24421 agaagaaaca tgttattaaa aattgtttaa aagaagcatt aaaaaatgat gggtttgaag
    24481 tgtactacca acctatattt tctaatagca ggggcaggtt tacttcagca gaggcattac
    24541 ttaggctaga acacaaagag ttaggtttta tacctcctga tgaatttatt ccaattgcag
    24601 aacaaacagg attgattaac tcaattggat tagtagtctt tgagaaggta tgtagattta
    24661 tagctagtga tgagtttgaa gatttaaaat tggataatat agctgtgaat ctttctgtag
    24721 tacaatgtat gcaaaaaaac ttagctgatg atttacttta tcttatgaat aaatataatg
    24781 tatcaccaac taaatttaaa cttgaaatta ctgagactgt ggctatgggc tcttttaata
    24841 taattaaaga aacaatggaa agattaattg atttaggtgt taagtttgca cttgatgatt
    24901 ttggaattgc ttattctgga gttactaata tgctttcttt accattttca gtaattaagt
    24961 tagataagag tttgatttgg tctatgaatg aagatagtag gcataaactt acagttgaga
    25021 cgattatagc tcttataaat aaattgaata tgaaggctgt agcagaaggt gttgaaacca
    25081 ttaagtgtgc tgaagattta atagctatgg agtgtgaata tttgcaaggg tattattttt
    25141 ctaaaccagt tccagaagat gtttttaaaa atttgttgaa agaaaatact tttacaagtt
    25201 aacacccttt atttttttaa ttacaacttt gttcattgac actgccagcg atatattata
    25261 aaattaacac aataataaat attaaattta ttatacatat tattaatatt aattaatgga
    25321 gtaaagattt attatatatt ttatttaatt atcctaaagt gagaaaggaa gatgtgacat
    25381 gaataaaaag aaaatattag tgccaattga tgggacagaa agaagtatgc attctttgga
    25441 atttataaaa ggaatattta aaaaagatga agttgaagta gaaataatga atgtaaaaga
    25501 actagtattt attgatggca tatccctggc tgaagagata aaaaattctg aaaatttagg
    25561 aagaagaatc ttggataaag cagcagagat aatgggagag tatgatgtta aagtacattt
    25621 tacttttgga tatccaggag atgagataat tagaaaagca aaagaagata atgtagattt
    25681 tatagttatg acaaaatcta ctaaaaaagg tcttacaaga atgataggtt cagtaacagc
    25741 aagtgtagtt aaacaagcaa aatgtatagt gatgattgta ccggagtaga attagaaagg
    25801 tatttgaaat atgaataaaa agatagtagc agtttgtgaa agtgaaagaa gtttaaattc
    25861 tcttaagaca gctgcaaatg atttaggatt tgatttagtt tatgaaattc aagaaaatga
    25921 taatataaaa aatgaacttt ctatttatga tatagaagat gcatccatag tgttatttgt
    25981 tacaagtgat tctatagaaa gcatagagaa gattgataga tttatagata gagaatttta
    26041 tgaagttgat ccaaagtata taatagagga tgcaaaatct atagtaagtg aaattttaat
    26101 agacttaaat taatcgtggg tgtcgcacta agaatagagc gacgcctttt tatatatttt
    26161 acacaagaaa taaagaggtt gtatcaaaat atagataaga gtctttttca ttgttgaaat
    26221 gatgaaaata tatttgattt catacaaaaa agagtacctc ataactaaaa agttcacttt
    26281 gagacactcc atttttatat gttagttgca tttaaggtaa aatagaagtt taatcatgtt
    26341 gaaataactg aattacatct tctaaatgat tgtacattga tgtataacct aagtaagagt
    26401 ctttactatc aaatgcaatc attatcttgt tatggtggct aatcatatcc ttgagagaat
    26461 ttctatattt aaaaccaaag tattggttag aagaaactgt tttaggtatt aactcataat
    26521 attttataaa aattggatta tgagaagcta aaattatctc tttgtgaaat tgcaagtcag
    26581 catcataaca ttcttctatc aaaccgtgag aaaccttatc caattcttct attgacttta
    26641 ggtaagtata ttttattttt tgaagttctt tagatgtagc atttaaaatt gctagtttag
    26701 tagcttcact ttcgataagt tgtcttgctc ttaaagattc tacataaaat tcttgtatgt
    26761 tatgattttt atagatttca ccatcatcaa ataggtcaac ttttttaaat ccttcaacta
    26821 taggacttga ataaggcaca gctctaagta ttccttcctt acagagtata tttatggcat
    26881 ttcttacagg tattctactt atgttaaata ggtcagcaat ctttctctca cttggtaatt
    26941 tactattaat tttaaaagtt ccatttagaa tgtttaattt aatataatca ataatttttt
    27001 cagtggcatt taagcttttc ataaaaactc ctaaacatat aattttttta tgattgtatt
    27061 atatcatagt taagagttaa atctactttt ttataatatg tataagacag gatagaggcc
    27121 aaaatagcta taatagccaa gataaagtat ccatattgta ttgaagtata ttccgatatt
    27181 ttaccaacta tcattggaaa tacagacatt cctatagcat aaattgcttg aaaaaaaccc
    27241 attgcagtag atttctgatg ttttggtaca tttttcatag actcgctagt aaggtaagaa
    27301 aggagtatac ctgtagatat acctggtagt acttgtagta caaatattat attaatattt
    27361 tttattgttg gaactaaaaa gcaataggct gaaactaaaa taaaaactaa aggaatccaa
    27421 aattttggcc catacttagt acaaaattta gtagaagcaa agccagaaga agcaacagca
    27481 cacaccatgt aaaatataga agatagacct atgagggtag aactagaacc taagttttta
    27541 agaatttgag tagtaaatga cattgttgta gacatttgaa taccctgttg aattaatgcc
    27601 ataaaggaaa aaaatacaag tcttttatcc ttgcagatag ataaatattt tttaattgat
    27661 attttttcag gtattttttt tgaattatga ttttttatat ttaaggctag aatcgttcca
    27721 ataactgaag acaatacact catgatacat aaggctgtca ttcctaattt ttcataaaat
    27781 aaagtactca taacaaatcc aatcaacatt cctagattgt ttactaatat tattgtactt
    27841 gtagcttttt gttgatgttc tgtagaaaaa aagttggtat agtgaaccat gtaagaaatc
    27901 caagttgatg atgatattcc agataaaatg ttagctataa gaaaaccatt gcctccagga
    27961 gaaaagactc ttataagaga tgctaaacca gcaaagaaaa ttccagacag tataaatatt
    28021 ttatgtttat ttatggaatc agcaaatact ccaacaggaa gccttaataa acattgtgag
    28081 attccatatg cacctattat tataccaatc atatttgtag atacattaca tgcacttaaa
    28141 tatggatttt ggaaaggtat atatacatat tgtgaaaacc agaaaaatgt aacaatagaa
    28201 agtaaaagta atttttgatt agatattttt ttcatattat cctccgtatg gtatttttat
    28261 aatgcaaata atataccatt tggatttttt aatcaagtac tttctctatt aaaaaaaatt
    28321 taaatatata attattattt acaaaaaaat tatactttat agttgaatta agaaaacgct
    28381 tatgatacta taaagttaac aaatttgaat atataacagt agacgtgtta ctggtaatgc
    28441 aggcaagatc gaatcaattt ttatagatta tctatagagt actatgggta taatctattt
    28501 atttggttcg atttttttgt ttaattggcc ataaaacaaa aagcctatag ttgaaaatgg
    28561 aggatttttt atggattata aaaatatagc tcaagaaatt ctactaaatg taggaggaaa
    28621 agagaatgta aatgaagtga ctcattgtat gacaagactt agatttaaag tcaaaagcgc
    28681 ttctaaagtg aataaggata aattatctaa aactgaagga gtaataactg ttgttgagag
    28741 tatgggtcaa atacaagttg taattggaaa taaagtaaaa aaagtatatg atgaggttat
    28801 aaaaattgta cctcaatcaa ataacgtagg taaaaagaat gaaagtcaag aaaagcaagg
    28861 tataattaat tctatattat cagcagtagc tggtatattt acacctacaa tacctgctat
    28921 agcaggggtt ggtatgatta aaggtatttt atcagttctt gctatgtatt atatgaataa
    28981 aaatggagta gatataaaag aaacgcaaag ctatattatc ttaaatgcca tggctgattc
    29041 tatattctat tttatgccta ttatactagg atatactgcg gctaaagtat ttaatgcaaa
    29101 taaaataata tctatggtat taggagctac actttgttat ccaactttta ctgctttgat
    29161 ggcaggtgaa gaatctgtta gatttttagg attggcagta acaaaagcta cgtatacatc
    29221 atctgttatt ccaataatta ttgctatatg ggctctttca tacgttgaaa aagtattaga
    29281 aaagtatata cctgaggtaa ttaaaataat aatggttcca actttatcat tggtagtaat
    29341 gttacctgca acattatttt tatttggacc aatcggaatt tatataggaa atgtcataaa
    29401 cttttcttat aagtatattt atgaattaag cccagcattg tgtggagcat ttgtaggtgg
    29461 gttatggtgt gtgttggtta tatttggtgc tcatagagca ttattaccaa taggtataag
    29521 tgatgttgct caaacaggaa gacagaattt attggcattt gcaggagctg caaacttttc
    29581 acaagcaggg gctgctctag gagtattctt taaaactaaa aatcaaggac taaaaacaat
    29641 ttctatgtct gcaactataa cagctttatt tggtataact gaacctgcaa tatacggtgc
    29701 caatcttaga ttaaaaaagc ctatggtatg tgccgtaata tgtggtgcat ttggaggtgc
    29761 gataatggga atgggtggag cttatggtaa tgcatttgca aaccaaggtg ttttaactat
    29821 acctgtatat gctgaagcag gtgcattagg tttcttaagt tatctaggtg gttgtgctat
    29881 agcatttttt ggttcagcta tttcaactta tctagttgga tttgaagatt tggaagagtc
    29941 ttctaataag gaaaattcat ctataaaagt agaaacgaag gatggtgtta ttgatataac
    30001 ttcacctgta gagggcgaat gtatagaatt gtcagaagta aaggatgatg tatttgcttc
    30061 aaaagctatg ggagaaggta tagctgtgtt acctacaaaa ggagtaataa cagcccctac
    30121 tgattgtgaa gtagcttctt tatttccaac attacatgca ataggattaa aattagataa
    30181 tggagcagaa atgttaatac atgttggaat caatacagta gagttaaatg gtaaatactt
    30241 taccaaacat gttaatcaag gagatttagt gaaaaaaggt gataaactta tatcgtttga
    30301 tatagataaa ataaaaaagg ttggttatga tgtaactacg ccagttatag taaacaatac
    30361 atttgatttt gggcaagtag tttcttgtaa atctagttat gtttcaacca atgataatat
    30421 aatttcatta gtattagctg gtaattaagt taatttgact agagataagt aaattgattt
    30481 aaaaggaaat aagtaaatca acaattaaat acatctaaaa gtaagtttat tgcaatgaat
    30541 tgttaaagaa aataattaat atggagtaaa tctattgcaa tgaagtaaca aagaaaataa
    30601 ttaatataga gtaagtttat tgtaatgaat tgttaaagaa aataattaat atagggtaaa
    30661 tctatttcaa tgaaataaca aagaaaagag ttaatataga gtgaacttat tgcaatgaat
    30721 caacaaagaa aataattaat aaaaaggaga aatatttatg aatacaggat ttccaaaaga
    30781 ctttttatgg ggagcatcat cttctgcatt tcaagtggaa ggagcttggg ataaagacaa
    30841 taaaggaaaa accgtagccg actataattc ttttaaaaag tctcatttac aggcagatac
    30901 taaagtcgca tcagactttt accataatta tgaagaagat attgaattaa tgaaagaatt
    30961 agggatgaaa acatatcgtt tttcaatttc atgggcaagg ataataccag atggtgaagg
    31021 agagataaac cagaaaggtt tagactttta taataagata atagacaaac taatagagtg
    31081 tgatattgag ccattcgtga ctttatacca ttttgacctt ccatttaaat tagtagagaa
    31141 atataatggt tgggaaagta gagaaactgt ctatgctttt gaaagatttg ctaaaatatg
    31201 ctttaagcat tttggagata gggtaaagta ttggcaacct cataatgaac agaatttaat
    31261 tgttagagtg gaggagagaa ttaatatata tgatgaaaca gattcatgga agatagataa
    31321 aatgcgtgct cagatggatt ataatctttg cttagcacat gcattagcag tcaatgcatg
    31381 tcatgagatg ataaaagaaa gtaaaattgg agcagcagta tcatcatctg taacatatcc
    31441 tcttacttct aagccagaag atatatatgc agctagaatg aatgataatt ttaaagtgta
    31501 ctatatgctt gatatgcatc attatggaga atatccaggg tattatatga agtatcttga
    31561 aaaaagaaat atagttcctc acatggaaga tggggataaa gagatactta aaaaagctaa
    31621 aatggatttt atagctgtaa attattatag gacaaattgt gcagaagctt tacctgaaga
    31681 ttcacaacat ccatttggat taagagaagg aactgttgat tttagtatgt atggactatt
    31741 taaaatgtca atgaatccta acttagaggc ttctgagtat ggagcagcaa tagacccatc
    31801 aggtcttaga gttgccttaa atgaatattg gcaaagatat cacttacctg ttataataac
    31861 agaaaatggt ctaggagcta aagatatatt agaagatgga aaaattcatg atgactatcg
    31921 tatagactat ttaagaagtc atataaatgc atgtaaacta gctattgaag atggtgtaga
    31981 gatgattgga tattgtccat ggtcatttac agacctttta agttcttctc aaggttttaa
    32041 taagcgttat ggacttgttt atataaatag aactgaccat gaaattttag atttaaaaag
    32101 aatcaagaaa gatagttttt actggtataa agaagttata gagaacaatg gaatagtaaa
    32161 ataaacataa atatatattg agggttttag tatgtacatt acaaaggtat taaataattc
    32221 gttgctactt gcaaaggatg ataatgatga agaaataata ttgatgggga aaggcattgg
    32281 ccataattat aaggccgggt ctgagttaaa tgaaaaagat atagaaaaga tttttgttct
    32341 tcatgatgaa aatataaaaa agagtattat acaattggca acagaaatag atgcagaata
    32401 ttttggtata gctcaaatga taattgcata tggaatagaa aaatataact taaaacttat
    32461 gaaccatata tatttggcct taactgacca cattgccttt gcagtgagac gttttaaaga
    32521 aggtataatg attgaaaatc actatttgtt cgaaatcaag gaatttaatc caaaagagta
    32581 tgatatagga aaatatgcta ttactgtttt taaggaggtt cttggtttag acctaccaga
    32641 agaagagatt ggtaatatag caggccattt tataaatgca caacaagata atccttatag
    32701 tgatagaaat aagagaagtg ctaaaattgt aaatgctatt ttgcaaatag ttcattatca
    32761 cttttctatt gtatataata aagagtcatt ttattatagg agatttgtaa tgcatctaaa
    32821 ggccttttca caaagatttc tggctaatga accatcaaga gaaaaaatag attttatata
    32881 tgagcaagtt caaaaaaatt gtaaggaaga gtatgaatgt gtgaaaaagg taggagctta
    32941 tatgtataag gaatactcca gagaattacc aagacaagag gagttatatt tgatgattca
    33001 tattcataag attttaggag aattatgcga aaatagttag aatatcttaa tatgctagta
    33061 atattataaa aaattaaaaa ttaactgcac tttaaatatg aataattgca ttacttaagg
    33121 gggttgtctt aaaattgatt tttttaatta aatttgaagc tcaactcctt ttttaaacct
    33181 ttatatcatt acaactttaa tgtttatacg cctgtacttc agatgtatat ctacttttta
    33241 catttctcaa catgcaaatc taccattctg tatatagtaa atgtattatc atgtttgttt
    33301 tattcaagca cacttataat acgcttgatt ttttcacaat gatgaaatat actaatgggt
    33361 gttataggta taagaatcgc attacacttg aagaagtaag tgataaatga gatataaatc
    33421 ctcatatgat aaactactat ttcattgagg aacatattct aagtgatgtc aagatactac
    33481 aatatgacta aattcctagt gataaaaaag tctacgaatg agtgtagaaa ggggaactaa
    33541 gatgaataaa gttttaatta tagatgatga taaagatctt tgtattttgt taaaaaagag
    33601 tatttcaatt gaacagattg aagcaaacta ttgccattct gggagagatg gactggttga
    33661 actttcaaaa caagattatc agttagtagt tctagatgtg atgatgccag gtatggatgg
    33721 ttttgaaact atggaacaaa tacgaaaata tagcagtatt cctatcttaa tgctgacatc
    33781 aaaaagtgat aattacagca aagtgcatgg attgcgagct ggtgcagatg actatctaac
    33841 caaaccattt gaaattacag aatttactgc tcgtgtactg tctttaatcc gacgttacac
    33901 ccgttttaat ccaaaaaatg aggagccaca gcaactttct ttcaaaggtt tgttgattga
    33961 ccttgaccag catacagtta ctaccaatgg cgaacctatg ttgcttccac ccaaggaatt
    34021 tggtgtactt ctttactgtg caaggcatca gggtaagatt ttgacaaaac agcagattta
    34081 tgaagccgtg tggggtgagc catatgtata tgatgacagc aatatcatgg ctattatcag
    34141 tcgcctaaga aaaaaaattg aactagaccc atcatctccc atctatttgc aaacaataaa
    34201 aggaattggg tatcaattca acacggaggt atgaaaatgg atgaacaaac gataattctt
    34261 ttcttactct tgctgtcgat tggtctactt tttctttcca tatttgcttt ttccaagctt
    34321 gggcatattc acaaacgctt aaaagatata gaggagatac tcgctgatgt acaaattgga
    34381 gacgaaaatc gtaaaatact aattaagcca tgtgatgtta tggctccatt ggtttatcag
    34441 cttaatgaga ttgtctacga ttatgaaaat aagttacttt ctctgaaaaa gtcggataaa
    34501 gctagcaaac aacttatgac aagtttatct catgatgttc gtacaccatt aacaactctg
    34561 attggttatc tggatgctgt acacagtggt atagttatcg aacaagagcg ggaggaatac
    34621 cttgaaatcg ctaggcgtag agcctacgac ctcaaggatt acattgatgt tctttttgac
    34681 tggtttcggt tgaactctga tgaatttacg ctgtcaatag aatctgtgga aattgcagag
    34741 ttgtcacgca atattttgaa agactggatt cctatattcc atgaaaagaa actgaatttt
    34801 gaaattgata ttccagaaaa tcgactaatg gtaaacctcg acccagatgg atattcccgt
    34861 gtggtcaata atctggttca aaatgttctt gctcacagta aggctcggca aatcaagatt
    34921 accatgagtg aggattccag aatggtatta ctccgtgttg aggatgatgg tgttgggatt
    34981 gccagagaaa atttaccaca tatttttgag cgactgtata agtgtgataa aggacgttca
    35041 gaaaaaggaa gtggtcttgg tctatctatt gtcagtcaaa tggtggagcg aatgggtggt
    35101 caggtttccg tagaaagtga gattggaaag tataccgtat ttacagtgag cttaccatta
    35161 atataaaatt gtttttcccc catgtgacat gtatacacag ggggattttt tgtaagttat
    35221 aaagttgcaa ggttaataca aggttgttgc aaggttaatg taagattgag gtggtatgat
    35281 aactttaata aacaaaggag gcttttatta tttatgaata atttaattat tgaaacaaaa
    35341 aatctcacta agcaatatgg tgctcaaaag agcgtttcta atttgaatat tcacgttcag
    35401 aaaggtcgca tttacggatt gctaggaaga aacggtgcgg gtaagacgac aactatgaaa
    35461 atgttattgg gcctaacaaa accaactact ggagaaatta aaatatttgg tcaggacatt
    35521 cacaataacg agaagaagct tcttcctcga attggatgtt tgattgaatc accagggttt
    35581 tatcctaatc tgacaggaac agaaaatctc aaaatctttg caaggcttag aggaatacca
    35641 aaaaaaaatg cagtagaatc agcactggat gtagtcggtt tgccaaacaa tgacaaaaag
    35701 ttattttctc aatactctct tggtatgaaa cagaggttgg cgattgctct tgccattatg
    35761 catgacccac aactgttgat tttggacgag cctatcaatg gacttgatcc tattggtatt
    35821 gcagaagtaa gaacgtttat tcgtgatctc tgcacagaac gaggtaaaac tattttgatt
    35881 tccagtcaca ttctttctga aattgccttg ttggcagatg acattggcat tattgaccat
    35941 ggtattttgt tggaggaaga aagtcttgcc caattagaag aaaaaaacag taaatacata
    36001 cattttgctg tatctgatgc agcacaggca gcaaggcttc tggaaaccca acttaacctg
    36061 aaaaatttca aagtagcaga taattacaat attatgctat atgatacaga tgtttctgtt
    36121 gcagaaataa atcgtgcttt cattctaaac aacattgagg tatcagaatc tcatttatgc
    36181 gaagatactc ttgaagatta cttcaagaaa gtaacagggg gtgaaggaat tgcttgatat
    36241 aatttcatgt gaattttcaa aactcaagcg gttaaaattt atactgattt ctattttagg
    36301 tgcttgctta tttccaattc ccgcaacaat tttgattgca aaggacaact tgccctttga
    36361 gcaattgttc aaattagttg tcaattttgg atatttcttg ctattaccaa ttgtattaag
    36421 tattgttgct agccaactat tttttataga aagagataat gatgtattaa aaaatcttgc
    36481 aacagtgcca gtgccaaaag gaaaactggc tctggcaaaa ttggctgtgt tgctgtttat
    36541 atcattgttc tactctgttg cagggttagg agctactatc attggaggtt tcattgttgg
    36601 aatagtagag ggggttgctg taaaattagg aatgagcatt gcttttggaa ttatgttttt
    36661 tgttactgta cttcctgttg tggtactcat tgtatatttc aatcgcagct atattttttc
    36721 tattatactt tcttttgtat atgctatttt caattttagt atatccttaa atattatcaa
    36781 ttttgaacca aataacccac tattaagcgt attacctgct cctgttataa tgcgttggtg
    36841 gatggcattt tggggagatc ccactggcga atacacggta cttagacagc catatttgct
    36901 ctcaactcct gcttgtgtag gtattttatg tttgataacc attatagcag tcctgctgat
    36961 ttgtatgata tacaaaaagc aggagaatta ggaggtatct atgcttgata taattactac
    37021 agaatttcaa aaaataaaaa gatataatat tctatggatt ggtgttgttg ctgttctgtt
    37081 ttcagcacta ttggcagttt ttcaggttgt aagttcacac ggcagtgacc ctctaacgta
    37141 tgagagcttt gcaaacggag ttatctggaa taatttcagt cttagttttc catttgggat
    37201 tacacttatt ggaggatatc ttattaaccg tgaatataca gaccaaacct taaaaaacat
    37261 cctaactgtt cctatttcat tacgcaaatt gctagtggga aaattaattg cggttggagg
    37321 aattactgta ttgttaagtc ttttcagctt cttatgcaca tttattttgg ggttgttatt
    37381 ttgcaatatc aatatatctg ttatactcgt tgtaaaatcc ttagttcaga ttttgtctgt
    37441 taatctgtat tgttatgttg ctgttttgcc gattattgct tattttggtc gtaaacaaaa
    37501 tgcatttctg acgggtgtag ggattgcatt tgtatacggt ttttgtggtg tgtttgttgc
    37561 tggaagaaat ctgacagact tttatccaat cacagcaggt cttggtctag ttggatacaa
    37621 caatggtgct ggtactgttt atcagccact tatcggtgct atgactttga ttgtaattct
    37681 aatcttgaca actattctac ttgtatttac acctaattat gataaagtta tgaccatatc
    37741 taagaaaaaa gggataaagc ataaaaagta ttctcataaa taacataaat attgtttcta
    37801 ttatttaagt tgctagacag tgaaaagaaa aaatctgtcg tacagtggct atatgctcat
    37861 gtattcaaga agctacagtt ttgatttttc ataactgtag cttctttttg tatttattaa
    37921 atcacgacta tcataatact tgcaataggt atagtaacaa gagagagtac tgtcatatgt
    37981 gttttatata ttacattaag tatattctaa aatatatgtt ttaaataata tatttgaaaa
    38041 taggaatatt taaataaaaa aaataatatt atatatagtt gacaaaaatt ggcaaatgat
    38101 atactatata aatatagaaa tagtaatcat tactattatt aaaaagggag gaattaatta
    38161 tgaacttaaa aggaactaaa acagaaaaga atttaatggc aggatttgca ggagagtcag
    38221 aagcaagaaa taaatataca tactacgctt caaaagcaag aaaagaaggt tataatcaaa
    38281 tagctgctat atttgaagaa actgctaaca atgaaaagga acatgctaaa ttatggttta
    38341 aacttttaca tgatggtatg ccttcaacag aggaaaattt aaaggatgca gcagcaggtg
    38401 aaaactatga atggactgat atgtatgcaa aatttgctaa agaagctaga gaagaaggat
    38461 ttgacaagat agcttattta tttgaagctg ttggaaaaat agagaaagag catgaagaaa
    38521 gatacttaaa gcttttagaa aacttaaatg aaggaaaaat atttaaaaga gatgaagaag
    38581 ttgtttggca atgtcaaaac tgtggtcatg tgtatgtagg tactgaagca ccagaaaaat
    38641 gcccagtatg tgaccatcca aaagcatatt ttaatataaa agctgaaaat tattaaaaat
    38701 tatcattatt aaaaaggaat gttaactatg aaattttcta aacaacgaga actgatttta
    38761 aatgaaatat taaataatcc ggttcatcct actgcggatt acctatatga aaacttaaaa
    38821 aaagataatc cgaatttaag cttaggaact gtgtatagaa atttagctca gctaacggaa
    38881 catggcttta taagaaaggt tagtattcca gggtatccag atagatttga tggtcgaata
    38941 gataatcact atcatatcat atgtgaagta tgtggagagg tatacgattt agaatccgaa
    39001 gttcttaata atttacaaga attaatatct gaggagacag acataaaaat aacatcttat
    39061 aacataagtt ttaaagggat ttgtaataat tgtaaaaggt gtagccaagt aggttagttt
    39121 tcaaattcta aaaataatta atgaggtgag tttatgtgta gtgaacaaaa attttttata
    39181 tgtgagactt gtggaaacct tgtaggaatg atacaaagtg gtggtgtacc tattttctgt
    39241 tgtggaaaac ctatgaagga acttgttcct aatacaacag atgcagcagt tgaaaaacat
    39301 gtacctgtaa ttgaagtgga tggaaataat gttactgtta aagtaagtag cacaactcat
    39361 ccaatgacta aagaacatca tatagcatgg gtgtatctta tgactgaaca aggtggtcaa
    39421 cgcaaatgtc tagcagtaga tggagaacct gtagttaaat ttgctttgaa tgatgatgat
    39481 aaagtgattt ctgcctatgc ttactgtaat ttacatggat tatggaaggc tgaactgtaa
    39541 tatttaaata aattgagtca aaaaggaggc tataaatgta ttttcataca aatagcctcc
    39601 tataaattta tataaattca agttttaatt ttgtttaatg tcaatataat ataaggagaa
    39661 aataatatgt caatttataa atgtagtgtt tgtggatata tttatgatga atctaagaat
    39721 gataaaacat gggatgaatt aagtgaagat tgggaatgcc ctgtatgtac aaaaggtcgt
    39781 agttattttg gaaagataag tactgtttac tatgaagaag atgaaaagat agcagaagac
    39841 atagttgaag atgaatctaa attaaatact gaaaaagagg gagatttaaa ttacttaagt
    39901 acatatttaa gaagagatga tgaagttgag aaacatatgg atataattca cgaaatggct
    39961 gttacaggaa aatctataat agaacctatg agaactaaac taccagtaat ttcttgggat
    40021 gatattttaa ttatgggtgc tcaattaaat ccattacctt taaatgagca tgatgaagta
    40081 aatacaacaa caataatagg aaaaaaagca aagaaaccta tgattataga aaatccagtt
    40141 tatatttccc acatgtcatt tggagctttg tctaaagaac taaaaatagc tttagcaaag
    40201 ggtgcagccc aaaataagac agctatgtgt agtggtgaag gaggtatatt accagaagag
    40261 aaggaagctt catacaagta tatttttgag tatgtaccaa ataaatatag tgtaactgaa
    40321 gaaaatctta aaaattctga tgctatagag ataaaaattg gtcaaggaac aaaacctggt
    40381 atgggaggtc atcttcctgg cgaaaaagta actgaagaaa tagcaaaagt aagaaataag
    40441 cctgtaggtc aagatgtaat aagtccatct tgttttgagg aaatacaatc caaagaggat
    40501 ttaaagaaac ttgtagatga acttagagaa gtttcagaag ggcgtccaat aggtgttaaa
    40561 atttcagcag gtcatattga aaaagatatg gaatttatag cttatgctaa acctgatttt
    40621 gtaactattg atggtcgtgg tggagctact ggagcaagtc caaagttgct taaagatgca
    40681 acttcaattc caacaatatt tgctctttat cgtgctagaa aatacataga tacacatgga
    40741 ttagatatag atttggttat aacaggtggg cttcgtatat ctactgattt tgctaaggca
    40801 attgctatgg gggctgatgc agttgctatt gcaagttctg ctcttatggc agcggcgtgt
    40861 caacaatatc gtatatgtgg ttctggtaag tgtcctgtag gagtagctac tcaggatgag
    40921 gaattgagaa aaagattaca tatagaaaat agtgctaata gagtggcaaa ctttttaaat
    40981 gtaagtttag aagaacttaa gacttttgct agaatttctg gtcataaaga tatacatgat
    41041 ttaagtgttg atgatttata tactgttaac tctgaaatat caaattatac aaacatacag
    41101 catgtataga ttgaagtttg aaactattag attttagaca gctataaaag taaatgataa
    41161 atatatttta taaaagagag tgtcttaaaa tgaacttttt agttcataag tcactctttt
    41221 ttgtatgaag ccaaatatat ttatcatttt agcaatgaaa aagaatctta tctctttatt
    41281 ttgagataga ctccttctat ttatgaaaat taaagatatt aaattgtaaa atattctgtg
    41341 atgtcgtact attaaactaa agattctgta ctttaaagta aataaaaatt taaggagtga
    41401 ttattatgga aagattgaag atacaagttt tagtcgacaa taatacttat attgatagat
    41461 actttgttgg agaaccagca gtaagctatt atattgagat tgatggaaat aggattttgt
    41521 ttgactcagg gtattcagat gtattcatat caaacgctga aaaattaaat atagatttag
    41581 gaaatttaac tcatgttgtt ttttctcatg ggcataatga ccatacaaga ggtatccagt
    41641 ttttagaaaa taggtatgat ttatctacag ttgaacttat ttcacatcct aactgtttta
    41701 tacctagaaa acatggagag aaaagcatag gagctccatt ttctgctgag gaaattaaaa
    41761 atatttttat gtataatcct aaggataagc catttaatct aagcaagaat tgtgtgtttt
    41821 taggtgaaat accttctata aatgattttg aaaaaagagc taaaattggt aaatgtaaaa
    41881 taggagattt gtgggaagat gattatgttt tagatgattc tgctatagtt tgtaaaactg
    41941 ataaaggtat ttttattgtt acaggatgtt ctcatagtgg aatttgtaat atagtagagt
    42001 atgctaaaaa ggtatgtgga gatgatagag ttattggtat actaggtggt tttcatcttt
    42061 ttgaattgaa taataggtta gatagtacaa tacaatattt ggataaagaa gaaataagaa
    42121 tgttgtaccc ttgtcactgt gtttctttta aggtaaaagc gaagatgaat gaaatattga
    42181 atataaatga agttggagta gggctgacca taagtatata gtatttgaaa gtatataaga
    42241 tttaagttga aggttaaaaa gatattttct atagaaaata tcttttttgt tacgtttatt
    42301 ttaagagaaa tcaatatact tttatatgaa atattgtaaa aaaatttatt tttaatgaga
    42361 atatattttc attaataaac aattttatta tgtattaata atgttagaat aaatataaat
    42421 aaatcaaagt tacttacatt acagtatatt ttttcacaaa atatattgat ttattctttt
    42481 aaagtttgat aattccaatg aatgtaataa aagaaggaat tttatactat actattatta
    42541 ttataaaata ataaagaaaa aattatataa taataaagaa aaaatgtata atatttaaca
    42601 ttaaaaaaga tatttcataa aattaaaaaa caaagtgaat ttgaatacta tttaaaaaat
    42661 aaaataaata tataaaaaat atttaatata aaggtagaaa taagttgaaa atgtaaagtc
    42721 attggaaaaa tatagttgaa tttaatgagc aaaaatgtta ttgttttatc aataaagtgt
    42781 taaaataaaa acttttaata tcattttagg gaggatgatt tcatgcaaaa aagtgtaaag
    42841 aaagcaaaag taacgggctc tatgttagcc atttttggtg ttgcaagcgt tctttttagt
    42901 tcacatgcag gaggaggatt tgctacaggg aatcaagaaa ctcagtacta tgtgcagtat
    42961 ggatggacag caccacttat ggcaatttta gcaatgatta tacttactgc taccatgaga
    43021 gaagttatca taatgtataa taataacaat tgtaggaatt ataaggattt attttgtgag
    43081 ctttggagac catatccaaa acttgaaata atatgggaga tatattacta tctaatggtt
    43141 ttaatagctg taagtgctgt tatagcaggt gcagcagcag tatttcaaag tataggagta
    43201 aattattttg tagctgtatt tattattgga gttgtacttc ttgtctttac tatatttggt
    43261 gctatgcttg tatctaaagc agctacagca atgacaatag ctattctagt atgtactctt
    43321 actattttta ttgttggtat aaaagctaaa gtaccagaaa taactgaaat attatcaaat
    43381 agaacgagtt tcacacctgg ctatatcaag ccaatactta atacttttat ttatgcaggt
    43441 tttcaatcgg ttgttatacc tacacttgca ggatgttcaa gaccacttct taagaactca
    43501 aaagaagcta ctaaggcaat gataatttca tttgttatga atgcaattgc attaggactt
    43561 gctgtaacta tgctaatggg atggtataga gaaattattg ctgctgggca aacaacactc
    43621 cctactcttt atgttgcagg tcaatctgga aatcatacta tatacataat ttataatgtt
    43681 gcattattcc tttgtttaat gtctacagga gtaactacta tatttggtct tgtaaataga
    43741 tttgaagacc ataaagcact ttctttcctt tcttcaagaa tgaaaagaag ggtatttaca
    43801 gcttgtgcaa taatggtagt gtcaatgctt atatcactta caggattaag taatattgtt
    43861 aaatatggat atggatattg tggttatctt ggtctattca ctatagtaat tccattcctt
    43921 acactaggac attataagaa taagaagttt gcaaaggaaa atccagaagc taaatggcca
    43981 gcggaattaa atgaaaatgt aaattagatg tagtagcaaa aaatattgct aaagagaata
    44041 ctatagataa gaatactata gataatatat ctgcatgttt attaagatta tttgcaaaat
    44101 ctaatattaa acaactataa acaagcaata taaaactaag aataaaagat aaaaagaggg
    44161 tgtctcaaaa tggactttta gtttataagg cactcttttt tatatgaagt caaatatatt
    44221 ttcattattt tgacaataaa agagactaat cctactttgg atagcctctt ttttctttaa
    44281 tataaaatag tataacagca aagtatagat tgatacatgt tttctgtagc tttctatata
    44341 ttacagtgtt tgtgtggcat tggtatttta agcaatctat aaattataat tctataattc
    44401 ttctactaat tttagattct atagccgtct ctatttcact taacattcgt tcatttttag
    44461 ggtcaataaa agcacatgta tagccttctt caccagctct accagttcta ccaatacggt
    44521 ggatgtagtc ttctggtgtt tctggtaaat cgtaattata tatatgactt actccagata
    44581 tatcaagacc acgagaagct acatctgtag ctattaagta ttgtatatct aaatttctaa
    44641 atgatttcat tattctttct cgttttgatt gagcaatatc actatgaagt ttttgacaat
    44701 tatatcctct tatagcaaga gcttcttcaa gattgtcaac ccttctctta gttctacaaa
    44761 atattattgc cataaaagga ttatcttcat ctagtacctt acataaggca tctaattttt
    44821 ttctatctgt agtttctact acttcttgtt ttatattatt aagagtgatt tcttcttttt
    44881 gaatagaaac tacaacagga ttttttgtgt atctataagc taattttttt acttgagaat
    44941 ttatagttgc agaaaagcat agtgtttgct ttttgtttga agtctctttt aaaatagctt
    45001 ccacttcatt tttaaatccc ataagcaaca tttggtctgc ttcatctaaa acaaaagtct
    45061 ttaacttact aaggtctatt gtttttctat ttagatggtc taaaagccta cctggtgtag
    45121 ctataatcat atgaatattt ccttttagtt tctttatttg agaaccaata tcttttcctc
    45181 catatgctgc aagaatactt acatctttag attctttaag tttcatagct tcttctgtta
    45241 tttgaatagc tagttctctt gtaggagaaa gtataagtac ttgtatatca tttatatcta
    45301 atgatatatt ttcaaatatc ggaagtaaaa aagctagtgt ttttcctgtt ccagtttgag
    45361 cttctgctat tacatctttt ccttctttta ttagtttaat actttctttt tgtataggtg
    45421 taggtgttgt tattcctgat ttttttagaa tatttattgt attttcacta atccctaagc
    45481 ttttgaaatc cattgttatc ttacctttca tgaaaaattt tcttttttat taaccaatta
    45541 ataatatcat atttataagt atttttagta aattttttaa ttattattta tatagcttaa
    45601 aaatatatta agatacttta aaatggaatg taacttaaat taaattttag taggaatttt
    45661 ataggtttat ttttaaaggt tttttgaaaa taaatctatt taaaaattaa attaaaatgc
    45721 aagaaaaaca taataacttt catttttaag aagttttcac aaaaaagaaa aaatatagtg
    45781 caataaatga tacaaagtaa tataatagat tacaagatat gaaaagctaa aaaaataaat
    45841 tgcaaatgaa attaaaatga ataaaaaata aaaaaataat cataagttag aggtatcaat
    45901 gaaaaataag acttaatgtt ttaaaattaa aagtatacta aggggggcat taatatgtgc
    45961 gttatatcaa aagatagagc aaaggaaata aaaatagtag atactacttt aagagatgga
    46021 gagcaaacag ctggggttgt atttgcaaat agagaaaaaa taatgattgc tgaaatgtta
    46081 agtgacctag gtgttgacca aattgaagtt ggtataccta ctatgggtgg agatgaaaaa
    46141 aatgtaataa agcacatatg tagtagaaat ttaaaatcag atataatggc ttggaataga
    46201 gcagtaataa aagatgttga agaatctatt tcttgtggtg ttgatgcagt agctatatca
    46261 atatcagtat ctgacattca tatagaaaat aaattgagaa cttctagagg ctgggtttta
    46321 gaaaatatgg ctaaaacagt tgaatttgca aagaaaaatg gattatatgt ttcagtaaat
    46381 ggagaagatg cttcaagagc agatatagac ttcttaactg aatttataaa tgttggaaaa
    46441 caagcagggg cagatagatt tagatactgt gatacagtag gagttatgaa tcctttttct
    46501 ataaagaatg caatagaaac tctttatgaa agaactaatt ttgatataga aatgcataca
    46561 cataatgact ttgggatggc aactgcaaat gcacttgcag gaatagcagc aggagcaaat
    46621 tatgttggtg ttacagtaaa tggattaggt gaaagagcag gaaatgcagc attagaagaa
    46681 gttttaatgg cacttaagtg tgtttataaa tgtgatttaa ataatataga tactagaaaa
    46741 tttagaggaa tatgtgagta tgtagcacag gcatcaggaa gaatacttcc tacatggaaa
    46801 ccagtagttg gggataatat gtttatacat gaatctggta tacatgcaga tggagcttta
    46861 aaagacccac ataactatga accatttgac ccaagtgaag taaatcttga aagaaaaata
    46921 gtaataggta aacattcagg aagagctgca gttgtaaata agttgagtga atatgaaatg
    46981 tatatttctc cagaaaacgc taccaagcta ttaaatgcta taagagctac atctataaga
    47041 ttaaaaagaa gtttaatgga taaagaaatt cttcaattat attgtgatat actagcacat
    47101 gagaaaggca caacagaaga agaagcagtt agaggttcat atatttaaga tataattgtt
    47161 taggaggaaa atatgggaga taatatagtt tataaaataa ttaaaaaaca tatagtagat
    47221 ggagaagctg ttgctggaag ttcaattggt ataaaaatag accaaacttt aacacaagat
    47281 tctactggaa caatgacata tcttcaacta gaagctatgg gaatagataa agtaaagact
    47341 aaaagaagtg tggcatttgt agaccataat atgttacaac aaggatttga aaatgcagac
    47401 gaccataagt atatacaaac agtggcagat aaatatggag tttatttctc taagccagga
    47461 aatggtattt gtcatcaagt atttttagaa agattttcaa caccaggaga tacactttta
    47521 ggttctgata gccacacacc tactgctgga ggtgtgggca tgatggctat tggagctggt
    47581 ggattagatg ttgctttagc catggcagga ggagcatact atataaaagc accaaaagtc
    47641 tgtaaggtga atctagttgg aaaattaaat aatatggtat catcaaaaga tataatatta
    47701 gaagtgttaa gaaaacaaac tgtaaaaggt ggagtaggta aagtttatga atatggtgga
    47761 gaaggtgtca aaagcctatc tgttccacaa agagctacaa taacaaatat gggggctgaa
    47821 cttggagcaa ctacttcaat cttcccaagt gatgaaaaaa cactagaatt ttttaagagt
    47881 caaggaagag aagaggcatg gatagagcta aaaccagatg cagatgcagt ttatgatgaa
    47941 gagattacta taaacttgga tgaattaaaa ccattagctg caaaacctca tagcccagac
    48001 aatgtagatg aagttgaaaa tataggcaag attaaaatag accaagtggc aataggaagt
    48061 tgtacaaatt cttcttatga agacttaatg aaagtagcac aaatcctgaa aggaaataaa
    48121 gtacataagg atgtgagttt ggtaatagca ccaggttcaa gacaagttat ggagatgatt
    48181 gctagaaatg gggctttggc agatataata agtgctggag ctagaatact agaaaattct
    48241 tgtggtccat gtattggtat gggtcaatct ccaggtactg actctgtgtc tttaagaact
    48301 ttcaatagaa atttctatgg tagaagtggg actttgtcag cacaagtata tttagtaagt
    48361 ccagaagtgg cagcagtaag tgcaatcaag ggagtgctta cagacccaag agaatttgat
    48421 attaaattta caaatttaga tgtaaatgaa tttttgatag atgatagcat gataattaaa
    48481 cctgctgatg taggaagtga tgtagaagta gttagaggac ctaatataaa acctttccct
    48541 cttaatacag agctaagtca gtctataggt ggaaaagtta tattaaagac agaagataat
    48601 ataactactg accatataat gccatcaaat gctaagttac ttccttttag aagtaatata
    48661 ccgtatcttg caaactattg tttcaatact gtagatacag aatttcctca gagagcaaaa
    48721 gacaataatg gtggatttat agttggtgga gataattatg gtcaaggttc aagtagagaa
    48781 catgcagctt tagcaccatt atatctaggt gttaaagggg taatagttaa gagctttgca
    48841 agaatccaca aggctaactt gataaatagt ggtataattc caatggagtt ttgtgatgag
    48901 aaagattatg aaaatatatc tcttttagat aatctggaga taccaaatat tttagacaat
    48961 ctaggtagtg gaattttaga agttaagaat acaactaaag gaacttcttt taaggtcaaa
    49021 gtagaattgt cagcaaaaga agttgatgta ttaaaagctg gtggaaaatt aaactacact
    49081 aaaaatcaag ctaattaata tgaaaagagg gaatattgaa tgtataaagt gacacttata
    49141 ccaggagatg gaataggacc agaagttgcg aaagctatga aaaaggtagt agaagctact
    49201 ggagtagaaa tagaatggga agaagttaat gcaggagagg cagttattga agagtatggt
    49261 actccacttc ctgaatatat tatagatagt ataaaaaaga acaaaatagc tataaaaggc
    49321 ccaattacta ctcctgtagg taaaggattt aggagtgtaa atgtaacttt aagacaggca
    49381 ttagatttgt atgtcaattt aagacctatt aaaagtttta aggggataaa atcaagatat
    49441 gaagatgttg atttagtggt tgtaagagaa aatacagaag atttatatgc tggaatagaa
    49501 cataaaatag gtgattatgc agctgaaagt ataaagataa taacaagaag tgcaagtgaa
    49561 agaatcgttg attttgcatg taactatgtt aaagataata agagaaaaaa agttacagct
    49621 atacataagg caaatataat gaaaatgtct gatggattgt ttttagatgt gtttagagaa
    49681 gttgcatcaa aacatggtgt agaatatgat gatttgattg tagatgcagc agctatgaac
    49741 ttagttttaa atccagaaaa ttatgatgta atggtaatgc caaatcttta tggagatata
    49801 ctatcagatt taggtgctgg gcttgttgga ggtttaggta taattccaag tgcaaatatt
    49861 ggtaaagatt gtgcgatatt tgaagctgtt catggaagtg ctccacaaat agcaggtcaa
    49921 aataaggcta atccaacagc acttattcaa tcatcagtaa tgatgcttag atacttaggt
    49981 gaatatgaaa atgcacaaaa aatagaaaca gctttagaaa aagtatttct tgaaggaagt
    50041 aaattgactg tagatttggg tggctcagct tctactacag agtttgcgga tgaggtgtgt
    50101 aagtatattg tatagatttt atttagggaa aaagtaaaat atagattgaa ggaaagaggc
    50161 tttctcaaaa tagagataag ccttttttat tggaatgata aaatatattt gattttatac
    50221 aaaagagagt tcctcataaa cgaaaaagtt aattttggga tatccatttt aattttaaaa
    50281 ttaattgatt aatatgcaaa gaaagattaa ataaatttat tatttttaat tgaactttca
    50341 accaaaatat ggtaaaattt aaaaaagttg aactttcaac taaaaatgaa tatattgatt
    50401 atgtgaaatt ttataaaaca gaaactattt gactttagcc atagtagttt cagtggaggt
    50461 gtgtttgatt aagactttag atagtaatat attaagagaa gtaggtactt tatctagagc
    50521 agtaaattca ataaatgata ttaagtacaa ggaattaaag ttacaaaaag gacaatttac
    50581 atttttgaca agaatctgtg aaaatcctgg tataaatctt gtggaattat caaatatgtt
    50641 aaaggtagac aaggctacaa caaccaaggc tatacaaaaa ttaataaagg caggatatgt
    50701 agataaaaag caggataagt ttgataaaag aggatataat ttaactccaa ctgataaatc
    50761 tttggaagtt tatgagttga taattgaaga agagaataga agtatagaga tatgttttga
    50821 taattttaca gatgaagaaa aacaagtggt aactaagtta ttagaaaaaa tgagcaaaaa
    50881 cgtagaaaat gaatggttta aagtaaagag atagtaaata aatacattaa cttaaggaga
    50941 tatttttatg attagaaaag cgaatatgaa tgatttagaa agcattatgc aaattataaa
    51001 atctacagtg gaagaaatga agacttataa taatacacaa tgggatgaaa actatccatt
    51061 agaaaaagac tttgtttcag atattaagaa acaagattta tatatttatg aagtggatgg
    51121 agaagtagca gggtttatat gtcttaatta tgaagagcca gaagaatatt taggcttaaa
    51181 ttggtcatct aataaaaaag caatggtaat acatagaatg gctgtaaatc caaactttag
    51241 aaagacagga atagcatcta agttagttga ttttgctgaa aaattagctg tagaaaacaa
    51301 tgtatcatac ttaaagagcg atacatattc aataaactca aaaatgaatt tattacttac
    51361 aaagtgtggt tttataaaaa tcggagaaat gagtttttta ggaaaggaga aatctttcta
    51421 ctgttatgat aaaatattat aaagagcatt tataatacaa gggataaata atagattttc
    51481 agcattagta agggcataat ataacctagt gtatgctatt atgaaaggga tgatttatat
    51541 gattggattt ctaaagaaaa gaagaagtat tagaaaatac aaggatgttg aagtagaaaa
    51601 agaaaaatta gataaaatat taaaagctgc tcttttagct ccatcatcaa aggggcttag
    51661 aacatgggaa tttatagtgg tagatgataa agaaaagcta attaatttat ctcaatgtag
    51721 aactaaaggt ggtggatttt tcttaaaaaa tgcaccttta gctattgtaa ttattgcaga
    51781 taaagaaaaa aatgatgttt ggatagagga tgcaagtata gctgcttcat acatacaact
    51841 tcaagctcat gaactaggtt taggttcttg ttggatacaa gtaagaaata gaatgcatga
    51901 tgataatata gaagctgata agtacattag agaagaatta aaagtaccta gcaagtacag
    51961 tgtagaatgt ataatttcta taggatatcc tgatgaagaa aagaaagcat acggtgactc
    52021 tgatttagac tataaaaagg ttcattttaa caacttttag tatataatat gttgtattaa
    52081 gacattatat ttttttctat tgttaatata tacttaaaat tgtaaaaata tatctgaatt
    52141 ttactaactt atctaggggt tatgtacaag gattttaaag ataaatatag aaattaatgt
    52201 ttataatcga tacataactt gagtaacaaa atttaaggag gttaaagtat gggtatattt
    52261 aaaacaaaaa tggatgagga ctggaaagta aactacatta aagagtttaa tgaaatgaga
    52321 gattcttatg agagtaaact tcaaaaaaaa cagtttgagg tagatagttt gaaatcagaa
    52381 ttggatagac taagaagcta taaaaatagc cttaagccaa aagaaaaaca aattactgat
    52441 gatgatataa ataatataaa gagtttaagg agagatggct taagctataa agaaatttca
    52501 aatcaaacaa gttggagcaa agcaactgta agtagggtat taaatggact ttacgattag
    52561 catatacatg gggtgattta aattgggatg aagtatagtt acaaaaaatg cataatagat
    52621 tctattttat tgatgtttat agttcaaata ttaaggatga ttttgaatta tgtacttcta
    52681 agtcaatttg aatttactct tgaaaatttt aatattataa acttaatttc ttttacatta
    52741 gttggtttat cgttaatttt atttttaaaa gataattctt tgtataacaa ggttagaaat
    52801 agaaaaatca cagaagcttt tgaagaaaat aaaaacaata tacttattga aaagtgtaag
    52861 ctgatacttt ttgttgtggt tttatcttta gcaataattg taacttattg tactaaggga
    52921 tatgtacttt ttaatgtaac tatgatgact ttgtctgtat tgatagtccc aatatttgaa
    52981 gagttatttt ttagagaata tatatggaac tatctaagta attttataaa gtcaaagagt
    53041 aaaattatct gtataacatc tatactatct ggaatttata atataggata catagatgtt
    53101 ataagaaatt atgtaatatt gtataacaat tcaagctaca cctttgaagt tattatttca
    53161 aaaattatga taggtactgt ttttggtatt gtacttggat tagttaagta taggtttaga
    53221 gatgtagggt tttgtatatt attgaggagc ttatttgcta tatttattag gtagatagta
    53281 taatcggttt ataaggttgt tttaaattta attttaaatc aaccttattt tatttgtgtt
    53341 ttataaagca cttatatatt aataactaat aaatttaata aaaattattt gaatattttt
    53401 aaaatatata aatatgtatt gacatataaa tattatgaat tacaatttat ataaactaat
    53461 aaaaattaaa taatattaat caacttattg aatggaggaa taaaaatgaa atttgtgact
    53521 ttttgtagta gcaatgaaga aaaaataggt gtatttaatt cagaaacaaa tagtatatat
    53581 gaaattaata gtttaggatt aagtaaatta tatactgata tgaatgattt tattgaaaac
    53641 gtatctacag gagatttaga aaaaataaaa aacaattcat ttgaaaacgc aaagtgttat
    53701 aagttagaag aagttaaatt atgttcccca attgtaagac ctaaaaaaga tattatatgt
    53761 ttaggtctaa actataaaga ccatgtcaat gaaatcccag atggagttat aaaaaacgta
    53821 gttatgccag attatccaat ttatttttct aagagagcag acaaaataat tggagtggat
    53881 gataagataa gcttacatgg agatttggta gagaagttgg actatgaatc agaacttgca
    53941 gtaatcatag gtaaagaagg tataaatata tcaaaagaag atgcttatga atatatattt
    54001 ggatatacaa tagtaaatga tataagtgaa agagcacttc aagataaaca tgttcaatgg
    54061 tttagaggaa aaagcttaga cacacataca tctatgggac cttgtatagt acataaagaa
    54121 gaatttgaac atcctttaaa attagatata agtagtgtag taaatggaga agtaagacaa
    54181 gattctaata cagagtactt tatatttgat atacctacta taataaatga tttatcaaga
    54241 ggaatgactc taaagccagg agatataatt tcaactggaa ctccagcagg ggttgctatg
    54301 gggatgaatc ctcaagtata tttaaagcat ggcgatatag ttgaatgtaa agtcgaagga
    54361 ataggagttt taaaaaatat agttgattaa tggataaata tgccatataa ttttattagt
    54421 acgaataatt taattaatat aggattaatc aaacttatga ttaatttatt atagagaggg
    54481 atgagtatat ggcaagagaa ctagaattaa agtatggctg taatccaaat caaaaaccat
    54541 caaagattta tatgaagaat ggagaacttc ctatagaggt attaaatgga aaacctggat
    54601 acataaattt tttagatgca tttaatagtt ggcaacttgt gaaggaatta aaagaagcta
    54661 caggtcttcc agcagcaact tcatttaaac atgtaagtcc agcaggagca gcagtagggg
    54721 tacctttatc agatacttta aagcaaatat attttgttga tgatttagag ctttctccat
    54781 tagcttgtgc ttatgcaatg gcaagaggag cagatagaat gtcttcttat ggcgatttta
    54841 tagcactttc agatgtatgt gataaggaga ctgcaactat tatagcaaga gaggtttctg
    54901 atggtataat agctccagga tatacagatg aagcccttga aatattaaaa ggaaagcgta
    54961 aaggcaacta taatattgta aaaatagatg aaaattatac tcctgaacct attgaaacaa
    55021 aggatgttta tggaattact tttgaacaag gtagaaatga aatattaata aatgaagacc
    55081 tattaaaaga tattccaact gataataaaa tttttacaga tagtgcaaaa cgtgatttaa
    55141 ttatagctct tattacatta aaatatacac aatcaaattc tgtttgttat gctaaagatg
    55201 gacaagtaat aggggtaggt gcaggtcaac aatcacgtat tcattgtact agacttgcag
    55261 gtaacaaagc tgatacttgg tatcttcgtc aacatcctaa agtattaaac ttaaaattta
    55321 aaaaggatat aggtcgtcca gatagagata acactattga tgtatatctt tctgatgatt
    55381 atatggatgt gcttgctgat ggcatatggc aaaacttttt tgaagaaaaa ccagagcctc
    55441 taactggaga aggaaaaaga gcatggctta aaactttaac aggagtagca cttggttcag
    55501 atgcattttt cccatttgga gataatatag aacgtgcaaa aagaagtggg gtaagtttta
    55561 tagcacaacc aggtggttct attcgtgatg ataatgttat attaacttgt aataagtata
    55621 acatagtcat ggcatttact aagaatagat tattccatca ttaaaatact gaaagtataa
    55681 agataatata gattaagtaa aaatctacaa caagttttgt tgtagatttt tttatttgct
    55741 gtataatttt aaatagaact ttatttttag atattcaatt tggaggttga tatggcttta
    55801 aatataagaa actatagcct attatgtaat caaggaacag ctaaatttaa tgaagatgta
    55861 gtcggtataa atccatttgg ggcatgggtt ttagatggtg ctacaggact taatggaaaa
    55921 aatttagtgt caactgaaag tgacgcaagg tggtatgttc agtggtggaa taggtattta
    55981 tataaaaata taaataaaga agaatcatta aagagtatta ttaatactgg aataatagaa
    56041 gtaaaaaaag agtatgaact aagattaaat gggtttaagg ttgaaaaatt agatttacca
    56101 tctagttcta tagttgttgt aaaatttcat gaaaataaag tagagtattt tttattagga
    56161 gattgtgctt tgtttattaa aaatggagat tccaaagtta taaaagatag gagtatttgt
    56221 aaatttgata atactgtatt tgatgaaatg agtaaacttt ctaacttgga agagatggca
    56281 tttgatgaga ttagaactag tgttatggat acaatagtaa gcaatagatt gaagaaaaat
    56341 acaaaagagg gctattggat attggatttt gatgaggaag ctgtggaaaa ttctatatat
    56401 ggatacattg aaataaaaaa agattttcaa ttgatgctaa cgagtgatgg ttttacaagt
    56461 gcatgtgata gatataatct aataaaagaa gaagaattga tacgtatagc agaaaaattt
    56521 ggtataggat atatacataa taaagtaagg gattttgaag ataatgacta taaagcggtt
    56581 aaagtgccaa gatttaaagt aaaagatgat tcatcttgtt tatatttaga tatatataat
    56641 gattagactg tataaaacta tttaatttta gaaaggaggc aagtgaaaag tgaaaattct
    56701 tcatataata acacaaaaac caaatagtac aggtagtggt atatatttgt ctggtatgat
    56761 aaaaggattc gaaaaaatcg gtcataagca agctgtaatt gcaggtatag atgtaaatga
    56821 tgatgtaaat tgttttccaa gtgaagtaag tttttatcca gtaaagtata attgtgggga
    56881 gttaaatttt cctgttgtag gtatgagtga ttctatgcca tatgaaagta caagatataa
    56941 agatttaaat atagacatga taaatagatt gaaatatcaa tttaaagtaa atatagataa
    57001 ggctatgaat gattttaaac cagaacttat aatatgtcat catttgtacc ttttaactgc
    57061 atttgttagg gaaatggtaa aagatataaa ggttatgtct atttgtcatg gaacttgttt
    57121 aagacagtta aataccattg atttagagaa agaatacata atagcaaata taaggaaatt
    57181 agatttaata tttgctcttc atgaaaatca gaaatatgat attataaaga cttttggagt
    57241 aagtcaaagt aaagtagttg taattggtag tggttataat gatgatatat tttataataa
    57301 aaattataaa ataaaggatg ataaaataaa aatagtattt gcaggtaaga tatgtaaatc
    57361 aaagggactg attccattta taaaagctat atctaaatta aaatatagta aagatttaat
    57421 agaagtcaat tttgcaggta ctggaagcga tatcgaatca tataatgaga tagttaaact
    57481 ggcaagtaag tctccattta aaatgaattt cctaggaaag ttagaacaga gagatttagc
    57541 tgagctattc aacaggtcac aaatatttgt gttaccatca ttttatgagg gattaccagt
    57601 tgtagtttta gaagctttat cttgtggcac agatgtaatc actactgata ttcttggtgt
    57661 taaagagtgg attggtagtg agattaataa ttctggaaag atagaatatg tttctctacc
    57721 ttttatggag aaagaaggta ttccaaaaga tgaagagtta tatgattttg aaaataattt
    57781 gtataatgca atagattcta agatacaatc tttattgaat aactcaaata agaaaacatc
    57841 tgtagatatg tcaaaaaaaa cgtgggatgg cttagcatat agaataaatg aagttatttt
    57901 aatggatgaa ttgtgtttaa tctaaaatta tttaaattat aaaaaagtgt aactattttt
    57961 acactatttt taaatgattt tatattatac taatactaaa tatatttgtt ttagggaggt
    58021 aatttatgag gattagtaaa aaggtattgg cgttaggtat ttcagcatta ctgttaagtg
    58081 ttagttttcc aacatcaata aacgcactag ataagattga aaatattcaa ggagtagata
    58141 aatatgaaac agctgggctt atttcagata agcaagattt tacaacagca gttcttatca
    58201 atgcagattc tacaatggta gatgggattg ctgcaagtgg tcttgcagga gtaaataatg
    58261 cagctatttt attaactaat aaggatgata taccagaagc aacgcttcaa agactgaata
    58321 gagttactaa gatatatgta ataggtggag aaaattctat aagtaaagat gtagaaaaaa
    58381 tgttacttat gagaagaatg caagttataa gaattgatgg tgtagataga gtagatacaa
    58441 gttataaaat tgcaggtgaa atagaaaaaa ttaaaaacag tgataagatg tttttagtaa
    58501 atggatttaa ggatgaagct gatgcagtaa gtgttgcatc tgttgcttat agagatggag
    58561 ctcctataat attgacaaaa gatatacctt cagcagaaga agacacagac ttagacccat
    58621 ggtttggagt ttctcctgtt ccagtatatg ctattggagg agagtctaca ttaagtgatt
    58681 acatagtgag cagatataga gcaactagaa ttggtggtgt tgatagatat caaactaata
    58741 aaaatgttat tgaaaagttc tataatggag caaaagagtt ctatataaca agtggagacg
    58801 atttagttta tgctttagtt gcatcaccac ttgcaaaaaa tgctcctgtt gttttagttt
    58861 caaataaaag tgataagagt atattaagtg gagctagtaa agtaacagca ataggtataa
    58921 gtgataagag tattatagag caatgtttgg atgctgtaaa gaagtaatgg agtgttatta
    58981 gtaaaatgaa tatacacatt gaaaattagt agattgttca ttttataatg gtataaaata
    59041 tatgggaaga ggctatcaaa tggagataag cctctttttc attgttaaaa ttatgaaaag
    59101 atactttatt ttatataaaa aagtatctta tgaactaaat gtctattttg atatactatc
    59161 tccttttctt atattatatg attttttata tggaataaat ttcataagta ttatcttcct
    59221 ttaatttttc taaccaaacc atctctaaaa tatctagttc ttctaaataa tctttctctg
    59281 ttttgttatc atcatgtttt ataagttcta aaacttcaaa tgtaaatgct ttttcaccat
    59341 attttttcca ttcattattt aattctttta tcatacatga gccagcacca agctgaaatt
    59401 tgagagagtt aaatttgctt tttatgtcat tactttttcc aaggtaaatc tttttagtaa
    59461 tatcattttt tattatgaat acacctgtgt caaatttcat ttctttgtat aattgtttta
    59521 attcacgttt tctatccatg attttagata cctctcttta taattgatat gtaatatgta
    59581 agtgtaaaaa tatattacat atcaattata aagagattaa ttttatatgt aaatagtttt
    59641 tataaattta attatgaatt agtcatcaat aaacaagaaa tttagctcta gaaatccatt
    59701 ataaaggcta ttataaattt taatatgcta aaaatataaa aagaatagtt gacagtgtag
    59761 aaaatatgga ataaactaaa agtgtaatag taatatgaaa ttatatatta cattgaaagg
    59821 tgtgaatgat tattatgaat aaaaaaatag aattaatagg gaattttgca agtgattgga
    59881 ttaggtatag tgattatgaa tataaaatta ctgaagaaga tgaattatat atagttccaa
    59941 cagaagatgc tgtgtttacc atgtataacc catttgatgt agcagatgat ataatagtag
    60001 atataattcg cataggacaa gaggcattac aagatatatc aaaagagaca aaagaaatga
    60061 aactaaagaa agaagtactt gaatttgtaa aaaagtatgg attattgggt ttaatatatt
    60121 caagtgtata taatagagat atagttggag aagaaagagt actgatgatt gaaaagaatt
    60181 atattacaaa agagaatata ttgagtacag atgaatatgt aaacatgttt ataccatttg
    60241 caagtgatga agatgtgtat tttaaaaagt atagaagggg tatagatgta tcaaagagag
    60301 aagaatctcc aaagtttttt ggaaagagac cattgatttt agatttagta ttttcaaagt
    60361 tctacactga acaactagat tggattgtaa gttttgctaa agatatgtgt aagcatttta
    60421 atcaaatttg tgtatataaa aatacatcta atctattaac tgaaagtgtt actattatgt
    60481 ctggagggtt taatcctcaa aagataggat tttctataag ccaattagat aaaccaatta
    60541 ttgcatggga gtttgattct ttgaaaacag ctattgagac tatctatgcc tttgcaatta
    60601 caagtgaacc ttcttcttta aataaatgca agtactgtgg taaaatttat attcctacaa
    60661 attctaaatc tcaatactgt tcaccttctt gtagaaattg ctcaaatgta aagaaaagta
    60721 gaagtagagc caaaaaagat aaataaaatt aataaatata tcattgttta gattttagta
    60781 aataactact gaattaaaag ttgttataga aagttatata tctaaatgat ggtatttttt
    60841 tgttgagaaa attgaaaagt ttaattgaga tagtaatttt gagttgacaa aaaccacttt
    60901 taacactact attataagtg gttggtaaaa taatataaat taatttaagt atagcataaa
    60961 aaacaaaaaa agtcaatgga aaaaataaaa aaggagaatt taaaaatgat atcacctatt
    61021 attttgagtt ctataaatca gaatttaaag gaaatagaaa gaaatgaact gcttgaaact
    61081 aatatagaat ctggagatta tggtctagct ctttctgaat ctgatgtaaa agatataata
    61141 aattcgagag ataatacttt aaaggggtat ggaagaatag aattagatat aaaagttaca
    61201 aaacagttaa tagaaaatat atatacatca caatatacaa atgtggacaa ttatttagag
    61261 gctattaatg atatgcaaga aattttttat tatttaaaaa atgaaactga tgacaagata
    61321 tgtgatgatg agattataga aatactgggt gaattttatg aaaaattttc aggcaatatg
    61381 gacaatgtaa gaggagaagc agacgaattt gcaaagaaat ttaaatttgg agaagtttaa
    61441 tatatgatag gagagatttt gtatgttaaa taatttaagt aatattaata taaacgaaaa
    61501 taatttaata aagaaccaat attccatatc tctacttaag gaatgtctaa attgtaaagt
    61561 gatagatgaa agagaagtat ataatataca acaagaaata tctctaatac ttatggattt
    61621 gattaaaaaa tatacaaatg gtcaaagcac atcagtaaaa acagaagttg ccgaaaaact
    61681 gttgatttca atatggtatg ctattgatgc atatataaat aagcttgggg atatagaaaa
    61741 aagagttgtg gagttgactg ctaagaatgt taaaaaagtg taccttggag gcattgaaat
    61801 attagaggaa gagttagtta ggctaaaaga tttttataaa ctaatgatga gtagaaaatt
    61861 ggatactaat tcaatagcat ataatgatac cttgatggag ttatcaagtt tctttaaaag
    61921 ttatattgta aaatttgaag ctcaggacgt tccagtaagt atagattatc cattggcact
    61981 agatgatatg gatgttaagg gtatttatta tgtgaagaat tatattgaga gtatagatat
    62041 agaaaataga ttttgtaacc tatttaagaa aaaagataga gaaaaattat tttatgacta
    62101 tggggtgact tataaaatag attgtgatgc tatacttgta aatttatttg aacttattat
    62161 aaataattgt attttttcta ctattttagg aaatgaagtg actaatttag aaatttcaaa
    62221 atatgagtat gaatttttag aaaaacaatt taaaaatatg tacaataata ttactgaaga
    62281 taaagttata gactctgaag aaagtaatat aattgaattg attgaagtag atgatagttt
    62341 taacactgaa gtagatagta atttggatta taatgatttt gaagaaatta acagagaaat
    62401 tagaataaat aaaaaaataa ctttactact tatggaagca attgaagtat taataaaaag
    62461 attaagcata gaagatgaag atgtaataag atatattaaa aaatatgaaa agatatttat
    62521 tgaatctgtg ctaagctcaa taaaaagtaa cacttttaaa agtatggtaa ctgtgagtaa
    62581 aaataaaaag gaacacttag aagattatat tgttgatgat gaaagtaaat tggatgatga
    62641 atcgtttaga gagatattca atgagatatt agatagtaca tctataattg aaaaaataag
    62701 aataataaag gaaaatatca atgctaaaaa agactttatc gatattcttg aatctgaatg
    62761 tttatttgga gaagagtatt taatgttgtt tgcatcatta agtgaattag aattggcaat
    62821 attgggtagt gtagtatttt atgaagatat tagaatgaga gaaattaata tattagaatt
    62881 tatgttaaat gagaaaactg aaactacttt atggaagata gagtacatta aatttatgaa
    62941 gaggcaaagt gaagataaga tatactccat agaagagcat atgaataaaa ttaattaaca
    63001 aaaaattgtg aataaataga atgttagtat ataatataat ctgtaaagca tatcaaaaat
    63061 acatatattt ggaggaaaat tatgaaagag tatatcataa ataatgttga agaaataaga
    63121 gaagaattaa ttgatttatc taagaaaata tgggaaaatc cagaattagc ctttgaagaa
    63181 aagtatgcaa gtagcattca aaaagaatac ctaaagtcaa aaggatttaa aattgaagaa
    63241 gtagaaaatt tacctactgg atttatagca agttttggag agggtaaacc tgtgattggc
    63301 atacttggag aatatgatgc tttaccagag ctatcacaat gtgtaagtgc agaaagaaag
    63361 ccattggtag aaggaaaagc tggacatgga tgtgggcaca acctgttggg tgtagcaggt
    63421 gtaggagcag ttgtttctat aaaaaaatta atagaagaaa ataaatttaa tggaacaata
    63481 aaatattttg gttgtcctgc tgaagaagaa ggtggaggaa agacagctat gtgtataaat
    63541 ggctgttttg atgatgtaga ttgtgcattt acatggcatc cttttgatat aaatgcacct
    63601 tggagaggtg gaagtctggc aaacttatct gtaaaattta aatttaaagg gataacagcc
    63661 catgcagctc aagctcctca taatggtaga agcgcacttg atgctgttga aattatgaat
    63721 gttggagcta attatttgag agaacatgtt attgactcta taagaatgca ctatgttata
    63781 actaatggtg gtggaagacc taatgtagtt cctggatttg ctgaaagttg gtactttatt
    63841 agaggtaaaa aagcaaagga tgcagaacac gttcttgata gattaataaa agtagctcaa
    63901 ggagcagcaa tgatgacaga aactgaaatg gagtataagg ttacagatgg aatatatgac
    63961 tatataccaa accaatgttt gactgattta gtttatgaca atatggtttt tgttggttgc
    64021 cctaaaaata ctcctgaaga agaagaattt gcaaaaaagt tgtgtgatac tcttactaga
    64081 gaagaaagat taggtgtagc aacatcatta caaaatgata aatctattgt agaaagctat
    64141 atacacaagg aaatagttga cggtgacaaa gataaaggac tagctggttc tactgatgtt
    64201 ggagatgtaa gttatgtaat accagtggct caatttgcaa tggcagcttg gcctgttgga
    64261 attgcaagtc atacatggca gtcatgttca tcagcaggtt caaatatagg attttctgct
    64321 atgataaatt cagctaaagt actggcttgt agtgcttatg atgtatttat ggatactaaa
    64381 attattgatg aagcaaaaat tgaatttgat aaatcattag atggacaaaa atttaaacca
    64441 ttggtataat tttgctaata aaatatttct ggggggaata gctatgagag aaaaagttga
    64501 aaaagtgctt gaggaaaaga taaaaccagt attacaaaga gatggtggag atgtagagct
    64561 tatagatgta aatgaaaatg gtgtggtttt agttagacta caaggggcat gtagtgggtg
    64621 tccaggagcg actatgacaa tcaaagctat tattgaaaat gttttggtga gtgaagttcc
    64681 aggagtaact caagtattag gtgtttaata gttagcgttt attaaatgta tgttatacat
    64741 aagtatttaa taaagaagta aaaaaaatag tgaagcaata tatatgaaaa agtatgagaa
    64801 agataataaa acattataga aataatgaaa tatttcatat gtatatgaag aaataagaaa
    64861 aaatagtgaa gcaatatata tatgaaaaag tatgagaaag ataataaaac atttatagaa
    64921 ataatggaat atttcatata tacatatgaa gaaatagtaa aacaatatat atatgaaaaa
    64981 gtgtgagaaa ataataaaat actatagaaa taattaaata ttctatatag gtattaagaa
    65041 acatgaaaga aataatggag tattataaat aaatatgtaa aagtgtgaaa gaaggatgta
    65101 gataaatttg tcttatacaa atttacctac agtatttctt acacactttt tattttacta
    65161 tccagccata ccaataaaca tacctataaa atatggaact aaccatatag tccatacaac
    65221 tatactaaac ttatgaaaga attttttctt ttcctcatca tctttgtata ggacaaaagt
    65281 agcccaactt gcatgaaaaa gcataagcac aattgcaaga agacctgtaa tactatgaag
    65341 gttttgtgat aaagcagatt ttacttcaaa agtatggctg ttggctatgt tactcattgt
    65401 aaatgttcct aaagtatcaa atataaaacc aagccaaaat ataattacat gtttcttttt
    65461 taaaattttt gcctttcttt ctccaaatac acctatagtg tagaaaatta aagctgatgt
    65521 tataaatact attgcaagta ttaatttata attcataatt atctctcctt tacttttgcc
    65581 aatatatttc taagattttt tactaagtca ctcaattcgt tttcatcaca atttttaaaa
    65641 atatcatcaa aactttcttg cattttaatt ttaaaatttg tggcaaattc aaatcctgtt
    65701 gttgtaaact taacgtatgt atttctttta tcattagatt tcttgaattt ttctatatat
    65761 ccaatctgtt ctaatctgct gattatgcct gaaaccgtac ctttagctaa agacatttca
    65821 tcacatagtt gtgatattgt aagttcttga ttatgagcta tcagttttat aactataatc
    65881 tgttggtgtg ttaatccatt ttctttaaaa ttttcttcta ctgtgtacat agttttagaa
    65941 taaagttctt ttaagagcat ggctattcta aaacagtcat aattactttt caataaaatc
    66001 acctcttaaa tggtttgcat acaaactaat ataatatact tttcttatat agtcaaccta
    66061 aaattttata attttataga aaataatgaa gaatagaata taaaaatttt attttaggta
    66121 taaataaaat aatttgatga aattttaaca atttttaaaa agtttgttta cacagttaat
    66181 aaatgatgct aaaattaact catagataaa tatagaaaat ttaattttgt ttaatgataa
    66241 tggaaccacg aaggttttaa cgttacttta cgtttgcttt tatgtggttt tttttattgc
    66301 aacaaagggg ggttggggtt tgttaaagga tatactgaaa aagttgttgc agtttatttt
    66361 agtaatgttt ttattatctt ttattgtttt ttacatggca agacttgctc caggagaccc
    66421 attaatctct tactatgggg atggagtaga aagaatgagt acacaagaaa aagaaaatgc
    66481 tatgaaaaaa cttggtttgg atgaaccaat atacagtcaa tatataaaat ggatttcaaa
    66541 tgcatcaaaa ggggaatttg gaatttcatt taaatataaa caaaatgtta cttctgttat
    66601 aaatgatgtc tatataaaca ctataatatt aggtggttca ggatatatac ttacctttgt
    66661 gctagcctta ttgttaggaa tattttgtac attacatgaa gataagctca ttgatagaat
    66721 catatgcaaa ttaggaacaa taaccaactg tattccatca ttttgggttg ccttagtact
    66781 catacttata tttagtataa acttaagtat acttccaagt agtggtgctt attcaatggg
    66841 agaagagtcc agtatattaa gtaggatatc acatctaatc ttacctctta cagtacttat
    66901 attaagccat ctatggtatt acacatatat gataagaaat aaattactag aggaaataag
    66961 agaagattat gtgcttttat gtaaagcaaa aggtttaaat aatagaacta ttgtttttaa
    67021 gcattgcctg agaaatataa tgccatcata tattagtata atggccattt cgatacctca
    67081 tatactagga ggaacttatg tagttgaaaa agttttttct tatcctgggt taggaacact
    67141 ttgttttgaa agtgcaaagt atcatgatta taatatgtta ttagttttat gcttaataac
    67201 aggggcttta gtagtatttg ggaatatgtt agctcaaatt ataaacaata aaatagaccc
    67261 tagaatgaaa tatgatagag gtgatactaa tgaagccaca atatagtgat tttgaaattg
    67321 taggagaaaa ttatatttta gtagcagaag atgaacatat agaaacaaag aaaaccttat
    67381 ctaaaaaaat taaagaatta ccatatatat caattattat tttatcaatc atagtcattg
    67441 gaagtgtatt ctcatctttt ataatgacac atgaaccaac atacatggat ttagcaagtt
    67501 ctaatttagc tccaaataaa acttttttat ttggaacaga ctcaatgggt agagatatat
    67561 attctatgat atggtatgga ggaaaaatat ctttatttat aggactattt tccactgtaa
    67621 tatcaactgt tataggaatt gtttatggta gtataagtgg ttcggcatct gaactcgtag
    67681 atgatgcaat gatgaggttt acagaaatca tacttagtat accttcaata ttaattataa
    67741 tatttgtaca agctatactt ggaaattcaa atccagtatc tatgtccata gtaataggga
    67801 taacaagttg gatgaatata tctaagatag tgagaacaga agttagacaa ataaaaaata
    67861 gcgaatacat actagcagct aagagcatgg gaggaggatt cttctatgta ttaaagcaac
    67921 atttacttcc aaattttgtg gcatcaataa tgttcatggt tgtaacaaac ataggcgcag
    67981 ctattggtac tgaatcgaca cttagctttt taggaattgg tttaccaata gaaattgttt
    68041 cttggggaag tatgttatct ttgtcagagg aagcattact ttcaaataga tggtggatta
    68101 tactgattcc gggtatattt ttggttacca cattggtgtg tataacaaat ataggtaatt
    68161 atataagaaa gaataataat aaaaaatcta gtaatttata aatcagcatt tgagtattaa
    68221 ataagcaaat actgaggtat ttgaaatatc tatttaaata aatggggtca aagctttgaa
    68281 aaatatttta taaaaagcaa aagggggagc atcatgaaat taaagaagtt aaaagtttta
    68341 agtttagtaa tgatacttag tctaatggca ggttgttcta gtggaggaga caaagataag
    68401 aaggcagata cgcctaaaga tggaaaggtg cttgtttatg gaagtaatga ctatacaagt
    68461 ataaatcctg cactatatga acatggagaa ataaattcgt taatatttaa tggattaaca
    68521 gctcatgatg aaaataataa ggtggttcct tgtctagcaa aggactggaa atttgatgag
    68581 gcaacaaata catatacttt taatttaaga gatgatgtta agtggcatga tggagagaaa
    68641 tttacagcaa atgatgttaa gtttacaata gaaactataa tgaatccaga taatgcttct
    68701 gagatagcat caaattatga agatataaca aaaattgatg tggttaatga taatactata
    68761 aaaattactt taaaagctcc aaatacagca atgcttgatt atttaacagt aggagtatta
    68821 ccaaaacatg ctttggaggg taaggatata gctactgatg aatttaatca aaaaccaata
    68881 ggtactggac catttaaact tgaaaaatgg gacaaaggtc aaagtataac acttgttaag
    68941 aatagtgatt attttgtaaa agaaccagga ttagataaag tagtctttaa gattgttcca
    69001 gatgataagg ctaaggcaat gcaattaaaa tcaggagaat tagacctagc tcaaataact
    69061 cctaaagata tgtctaactt tgaaaaagac gaaaagaatt ttaaagtaaa tataatgaaa
    69121 acagccgact atagaggtat attatacaat tttaattcta aattctttaa agataaaaaa
    69181 gctaaaggat taccaaatgc tttaagctat gcaatagata gaaaagcaat agttgacagt
    69241 gtattattag gacatggtgt accagcatat tcacctttac aaatgggacc atataataat
    69301 cctgatatag aaaagtttga atataatcca gaaaaagcta aacaagaaat tgaaaagcta
    69361 ggttggaaat taggttctga tggaatctat gaaaaagaag gtactaagtt agctttcgag
    69421 ataacagcag gtgagagtga ccaagttaga gttgatatgg ctaaaatatg tgcacaacag
    69481 ttaaaagaaa ttggtgttga tgctaaagct gttgttgtaa ctgaaacaga ttgggctaat
    69541 caagatgctc atttaatagg ttggggaagt ccatttgacc cagatgacca tacatataaa
    69601 gtatttggaa cagataaagg tgcaaattac agtgcttact ctaatcctac tatagataaa
    69661 atacttcaaa aagcaagaga aacagaagat aaagatgaaa aattaaagtt atataaacaa
    69721 ttccaagtag aaatgactaa ggatatgcct tatacattta ttgcttatat agatgcaata
    69781 tatgttggaa aaccaaacat aaaaggatta acacctgata cagttttagg tcatcatggt
    69841 gtaggtgtat tctggaacat agctgactgg acaattgaat aatactgaaa ttaattagaa
    69901 accttaggga taaaaatata tccctaaggt ttgaatagca taggggttga tggtttttgg
    69961 aacatttatt ggaggttaat aatctatctg taagttttaa agtagaagaa ggagaagttc
    70021 aggctgtaag aaatgtatct tttaatctaa aaaaaggtga gacattggct atagttggag
    70081 aatctggttg tgggaaatct gtactgtgta agagtttgat gaggatactc ccatacaatg
    70141 gatatattaa aaatggagag gttttactta aatcaagtga tttggttaag aagtctgaaa
    70201 aagagatgga agatataaga ggaaagaata tatcaatgat atttcaagac cctatgacat
    70261 ctctcaatcc aactatatca ataggaaagc aaattgcaga agctgtaatc attcatcaag
    70321 gtataagtaa aagtgaggca aaaaagagag ccatagagct tattgaattg gttggaattg
    70381 ataatcctga gaaaagattt aaacaatttc cacatcattt ttcaggtgga atgagacaac
    70441 gtatagtaat agctatagca cttgcttgta atccagatgt attgattgct gatgaaccta
    70501 caactgcttt agatgtaact atacaagctc aaataattga tttaataaag gatttacaac
    70561 ataaaatagg tttgtcaata atatttataa ctcatgattt aggagtagtt gcaactatag
    70621 ctgatagaat agcagttatg tatgcaggaa agattgtaga aataggaact gtagaggata
    70681 tattttatga ccctagacat ccatatactt gggggttgtt gggttcatta cctactttag
    70741 attcgcagga tgactattta tataatatac ctggaatgcc tccaaattta ttgaacccac
    70801 caaaaggaga tgcttttgca ataaggaata aaaatgcact taagatagat tatgaaaaag
    70861 agccaccaat gtttaaaatt aatgatacac atagtgcagc aacttggtta ttgcacccag
    70921 atgcacctga agtagatgta ccagttagag taaattgtgg aagggtgatt tctaatgaat
    70981 agagaaaaaa tactggaaat aaaaaactta aagcaatatt ttcatttgga caaaagtact
    71041 actgtaaaag cagttgatga tataagtttt gatatatata agggtgagat atttggattg
    71101 gtaggagagt cgggttcagg aaaatctaca actggtaaaa ctataataag actacatgaa
    71161 tctactggtg gagaagtaat ttacaaagga aattgcatat cagataaaaa aacgtacaaa
    71221 tttataaaaa aagatgtaaa caaaagtatg cagattattt ttcaagattc tacttcatca
    71281 ttaaatccac gtatgacaat tgcagatata atttcagagc cactaaaaat tcaaggtatc
    71341 tgtaaaaata aaacagatag aatgaataaa gtatatgaga tgctaaagct agtaggactt
    71401 gatagaagtt atgcaaataa gtatccatct gatttttcag gtggacaaag acagcgtatt
    71461 ggcatagcac gagctctttc agtagaccca gaatttataa tagctgatga gcctatagct
    71521 tctttggatg tgtctataca agcacaaata gtaaatttat ttaaaaaatt gcaacaagaa
    71581 aagaatttaa cttgtttatt tatagctcat gatttatcta tggtgagaca tattagtgac
    71641 cgtataggag ttatgtataa tggaaaatta gtagaattgg cagattcaaa tgaattgtat
    71701 aataatccta ttcatccata tacaaaatca ctactttctg caattcctgt tcctgaccct
    71761 agatatgcta agtcaagaaa tagaatagag tataattcta atggatatga ttgttcaaat
    71821 gaaaagtcat taagttggat tgaggtttca gatggacatt ttgtatattc ttctaaatca
    71881 gaaataaata aatatcaaca aaatttaaaa gttgtttagt ataaaaatac ttgaacaggt
    71941 atttatttga aatacaaact aaaaccctat ttttggagaa attttaaaac tactccaaaa
    72001 tagggttttt tatttctact tttcaaatat aataaattca aatataataa aattaaaaac
    72061 aagtaaagta tgttgttaag aaagtactat aaaattaaaa atagtaatgt agaaaaaatg
    72121 agcaataatc ttattttttc tacaataatt ttctataatt taaagtaagg aaagtttgat
    72181 aaattataac cttagttaaa gaataagagt ttattaaaaa ataaagtgtg tataggggtt
    72241 ttagttaata aaatactata atgtagggga gcttgtaatg atatataaaa aagataaagt
    72301 aatgaaagtt ttagaatcat catataggga aaatgaaaaa gggattacag ctaaccaatt
    72361 atcagaaaag acaggaattg caagaaacaa tataagtaca tatttaaacc aattattcaa
    72421 tgagggtatg gttacgaaga taaaaggaag acctgtgtat tatatccctg caaatcttaa
    72481 ccttaacaag aatgaattgc ttgatacaca aagtgagttg cttggcataa aaaaaggaac
    72541 aaaagtagaa aacaaaataa aaaaagatag ttttgaaaca ctaataggta aagaacatag
    72601 tttaagacct ataatagaaa aatgcaaggt cgcaatgctc tatcctacaa atggacttca
    72661 tactattatc tatggagaaa caggagtagg aaagagtatg attgctagat atatgtacga
    72721 ttattctata gatagtggaa ttagaaaaaa taaagctcct tttgtaacat ttaattgtgc
    72781 tgattatgct aataatgcac aattacttat gggacatatt tttggggtag aacaaggagc
    72841 ttatacaggt gcagaatctt ccagaaaagg tcttttggaa attgcaaatg gagggatact
    72901 atttttagat gaaattcata gactaccagc agaaggtcaa gaaatgttgt ttacttttat
    72961 agataaggga acatttaaaa gactaggaga tgcaacgaaa gaaagagagt ctaaggtatt
    73021 aataatatgt gctactacag aaaatccgac atcaacacta ttagatacat tcaatagaag
    73081 aataccaatg aaaatagaaa taccatcatt gagagatagg acaatgattg aaagaatgga
    73141 attagtcaaa aattttttca aggaagaatc aaaaagtatt tctatttcaa taaaagtaca
    73201 taaagaaata attaaatcac tattacttta tgattgtaaa aataatgtag gtcaacttca
    73261 aaatgatata aaattagctg ttgcaaatgg atatctaaga tataaaagag ataataaaga
    73321 accagtttat atagagaaaa aatattttga taaatctata aatgaagtga gtgatgatta
    73381 tagagagaag tctattaaag cagatgaagt tataacacat ggaatagaat attttgtatt
    73441 tacgtcatta ggagaagaag aagttattaa ctacaatgat ataaatatta ttaatgcagt
    73501 aaatactaat cttaataaat ctgtagaaat gaatatagca gatgcttctc tatttaataa
    73561 tgttaagttt aaaagcatgt gtgaatcaat aagacaaatt attaaagaag atgaagaaat
    73621 agacataagt gaatcaatat ttaagtcaat ggcaatttat ataaaaagcg tattggataa
    73681 aagtagctct acaaacaaca tagatttgaa tcaaattaga agaaataaca gaagtgaatt
    73741 taaaacagca ttaaaaatag ttggaataat tgaaaatgaa tttgatttat ttttatctat
    73801 agaaactgta gcatatatta cattattcat agtacaagct aaacaagatg aacaaaataa
    73861 aatagtgaat aacattaata ttatagttgc tatgcatgga gaaactacag caagtagtat
    73921 ggtaaaagca gttggtgata ttattggaaa gtgcagtgct atttctttcg atatgaagtt
    73981 agataaaagc tatgatgaag ttattgtaga ttttaaaaat ttaattaata atatagacaa
    74041 taaagatgta ttactattta cagatatggg ttcattaaac tcctttgatg aaattataaa
    74101 gaaagaaaaa aagtgtggag taagagtaat tcctatggtg acgacattaa ctgtgttaga
    74161 agcagttcag aaggccaata tgggattacc tttaaatgac gtatacaact ctatcactaa
    74221 cacgagaaag cactattttg gaactaatga aattcaaaat aaagaaaatc tgtctaaaac
    74281 aattattata gcatcacatg tttcagaagg tgtagataat aaaacaagaa aaatattaga
    74341 agaaaagatg agtaggtact tagatggaat agatattata tctgtcccat ataaaacaga
    74401 aaaagattta agtttaaata ttacgaaact taaagaaagt agtaatattg ttgcagtaat
    74461 caatgaacaa agaattaata ttagaggaat tgactatatt agcaaaaaag atatagataa
    74521 ggatgaaaat atcaataaat taaaaaatat tataaagata agtataggtt atgatgatgt
    74581 agttgaaggt ttaaagacat ccttaaaaag ctctaattat aatagaatat ttaaagatat
    74641 aaaatatgtg tctgatgaat tgtttttagt atttaatata gaaaagaaat acgataaagt
    74701 aattggactt atgatgcatt tagctttcat ggtagatggg ttgattggaa atactagaga
    74761 aattgaaaag cttgataaag aaaaaacact tgattatcac aagagtctaa gcaaaataaa
    74821 agatatagta tctcaattag ataaaaaata taatatagaa attaatgaaa aagagtgcta
    74881 tcaaatatta ttaatattag aatatgcaga aataatcgaa aaagattacc aatgagtata
    74941 aaaacttatt tggtaatttt tttttagtct aaaataatat taatattaga cacatataaa
    75001 tatacttaga caaagtatct aagttttgat gataaaaatg attgaaataa cagtgtttta
    75061 aagtgtctaa aaaaactggc ataatacttg cttatattat taaatgagta atataaataa
    75121 ttaaaattta aataaaaagg agtaaataat atgcaagata aaatattaag agataaaaag
    75181 gcaaatgacc catgggcaag aatgactaca agaaacggat ttagtgcaga cgaaataata
    75241 tcgtcatttc aaaaagctat aagaagaaat atggtggaag aagcttgtga atttgcttat
    75301 gaactatata tatcatcacc agaattagaa gataagctat ggagaaggat acttacaata
    75361 tcagttgaag atataggaat gggggatcca tcagctgtaa taatcataaa taatttaaat
    75421 caagtaagaa aggaatattc atatgcagat ggagacagac ctttattttt catacatgca
    75481 ataagatatc tttgtgcatg tgagaaagat agatcaagtg atttattaaa aaatataatt
    75541 ataaaaggat ttgcaatggg taaagttcca gaagttatgg acgtggcact tgacaagcat
    75601 acatatagag gattagaaat gggaagggat tcattccact ttttaaatga agctagtata
    75661 gtaataccag aaaaagaagt agataatgat tataaagaaa gatacttaaa aatattagaa
    75721 aaatataagc aagaagaagt tatagatacg gctttcaagt ttaatcattg gcagtactaa
    75781 gaattagagt aaatattaat aagaattaaa ataaaataat aagaattaaa gtaaaaactg
    75841 ataggaatta gagtaagaat tggctgataa aaaattagaa aataaaagag gttagatatt
    75901 gtaggagggt aattatgaat attttattag tatgtgcaaa tggagcttca acaggtgtac
    75961 ttgtggaaaa gatgaaaagt ttttgtagtg agcatgaaaa gttaaaaact aaaactataa
    76021 atatagaggc aacatcattt gaaaatttaa aatcatacat tgaagctaat gatacagatg
    76081 tagtacttgt agcaccacaa attaggttta aggaagatga tgtagttgaa gcttgtaaga
    76141 attacaaaat agcagttggt ttaatagata ctaagcatta tggtcgtatg gatgctcctt
    76201 ctgttatgaa gtcggcaatc gaattatata aaaatagata attgggggga ataaaaatgg
    76261 aagcacttca aaataattta agaagatttc ttcttccaat agcacaaaag attgaaaaac
    76321 aaaggcatct tcaagctata aaagaaggta tgataagcat taccccaata ataattgttg
    76381 gttctttaag tatgttattt atggcattaa acaatatgtt acctgaagga agtgcaaaaa
    76441 cattattatc tgaaaatatg gatacattac ttatccctaa taaatttact atgagtttat
    76501 tatctatata ttcagcattt tttatagctc aggcactagc taaaaaatat aatcttaatc
    76561 atgtagaaat aggcatgaca gcagttgttg cacaacttgt tgtatgtgga caagtagtag
    76621 atggtgtatt agatacaagt tatttagatg cacaaggttt atttgtaagt atattagtag
    76681 cacttttagt agttgatatt actaagttta tgaatgataa aaatctcgta attagatttc
    76741 caaaagaagt acctagtgta gtaaataaaa gttttagaaa tttaactcca atgatagtat
    76801 gtataatgtt atttacagca atagcagcaa taactaaaaa tgtctcaggt caaccactac
    76861 cagctattat aatgaatttc ttagcaccag ctataagcag tgtagataat gtttttgcag
    76921 taactataat actatttata actcaattat tatggttctt tgggcttcat ggagcagcaa
    76981 taacttcaag tatatggatg ccaatagcag ctacatatat ggcagaaaat gcaacactga
    77041 tagcagcagg aggagaccca aaatatgtgt ttacaatagg attttactat ggtttcttgc
    77101 aagtaactgg ttcaggaata actttgggat tagtgtacct tatgtcaaga agtaagtgta
    77161 agagttttaa tagtatggga aaagttgtga ttttaccatc actatttgga ataaatgagc
    77221 cggtaatatt tggaacacca atagttatga acccatatat gtttgtacca tttgtatttg
    77281 gacctgtatt ggttggagca cttaatttta tggcattaaa agtaggatta gtaggattac
    77341 ctattgcaga accaccaggc tttttacctc caggggttgc agcatttttg atgacattag
    77401 attggaaggc aatcgtatta gtatttgcaa gtataatact tatgaccttg atatattatc
    77461 cattctttaa gataatggag aaggaagaat taaataaaaa tgcagaatta gctacaacat
    77521 tagatgatga tagctttgat ttttaacaag tcattagaag gagaaggata tggtacctga
    77581 aatggaagaa aaagtattta gtataatatc agaagcagga gatgctaaaa gtgatgtaat
    77641 gatgtctctt aaggaaataa aaaaaggtga ctacaataaa gcaaaacacc ttttaaattc
    77701 agcctcagag aaaatacaaa cagcatcaaa gcatcattta gaattactat caaatgctat
    77761 gaatagtgaa gatagtggga ctgatttttt agttgtgcac tctgaagacc attattcaaa
    77821 tgcattattt gctcattctt tagtatctga acttgtagat atatttgaga tgatggaccc
    77881 aagaattaat aagaattaaa tttcttgtaa atagtgcttg aagtttggct atagtttatt
    77941 aaatcaatag atttaataaa tgaactccct tttactttaa agtactagta tttataaatt
    78001 tatttataaa tacagtaaat ttttaaatag aaaaggagat attattatgg aaaaattttc
    78061 agtagtaata gctggaggag gaagtacata taccccagaa atcatattaa tgttgctaga
    78121 taacttagat aggttaccat taagagcaat caaattatat gataatgatg aagaaagaca
    78181 aaataaggta gctaaagctt gtgaaatact aataaaggaa aaggacccta atatagagta
    78241 tttagcaaca acatgcccta aagaggctta tacagatgta gatttttgtt tagctcatat
    78301 aagagttgga aaacttgaaa tgagagaact agatgaaaag atacctctaa aacatggggt
    78361 agttggtcaa gaaacttgtg gaccaggtgg aatagcttat ggaatgaggt ctatagaagg
    78421 tgttcttgaa aacatagatt atatggaaaa gtattctcca aattgttgga tgttaaatta
    78481 ttctaatcca gcatcaataa tagcggaagc tgttagaagg ttgagaccta attctagggt
    78541 aataaatatt tgtgatatgc caataggtat ggaacataat atagcaagga ttgcaggact
    78601 caaatctcgt aaacatatgg atataagata ctttggatta aatcactttg gttggtatac
    78661 ttcaattaaa gataaaaaag gaaatgaact tttaccaaaa ttagtagaac atataaaaga
    78721 atatggtttt ataaatggtg aagaaggaat gaaaaatgac aagaaagata gttggtttga
    78781 aactaattta tttactaaag aaatagtaaa aacagattta actacaatac caagtagtta
    78841 cttaaagtac tatctatttc cagattatgt tgtaaatcat tctgatgtaa attatactcg
    78901 tgccaatgaa gttattgatg gaagagaaaa agaagttttt ggttcatgtg cattgataga
    78961 aaagcaagga cattctaagg gcagtgggct taagatagga atacatgcag agtttatagt
    79021 ggatttagct acagctctag cttttaatac acaggagcgt atgttattga tagttcaaaa
    79081 taatggagct atagaaaata tacaagatga tgctatggtt gaaattccat gtatagttgg
    79141 aaaagacggg tatgaaccat tatcaatagg gaaaatacca acattccaaa aaggtttaat
    79201 agaacaacaa ttagcagttg aaaaattagt agttgatgct tggattgaga agtcttataa
    79261 taaattgtgg caagcactta tgctatctaa gacagttcct agtgcaacag ttgcaaaaga
    79321 attactagat gatttaatag aagcaaataa aaattattgg ccaaaactta attaagaata
    79381 atatattttt aaagttccta cagtttagta catagcctag ttaatgtatt tgtagggact
    79441 ttatttatct acaaatgaga tacaagtggc aaacatagtg tattttgcag ttaggaaagt
    79501 aggtttagtt acttgttagt tggattaaat ttaggtacaa taatttacta tcaatcaata
    79561 gaaataacca gagaagttgt ataataaaaa atttacactt ttaacaggat ttatgtaggt
    79621 gtttatagaa ataaataaat atgtacataa atacataatt atttttaagt ctaaaataca
    79681 agaatggaga gatggctatg aagtggagag attatgctga tgatgtaaat ttatttgagg
    79741 attttgataa aactatcaag cctctaactg acgaacaaag aagaaaaaat ataaacactt
    79801 taattgcata tttttcaaaa gaagttcctt ctaaaatata caatttatca aatgatgaaa
    79861 ttaagcctag agatatactt agaggacttt tgaatgtata tccaccaaaa gaaattgctc
    79921 cagagatact taatatgtta cataatttat tgttgattga atgtgaagaa agagagttag
    79981 tagatgtaaa tgatatagaa gaagtagagg aagggattgc catatggaga ggtaatataa
    80041 cgaatttaag agcagatgca atagtaaatg ctgcaaataa caagttatta gggtgtttac
    80101 aaccattaca tctatgcgtg gataatgaga tacattcatg cgcaggtcct agacttagag
    80161 aagattgtga caaaataata aaaaaacaag ggcatttaga atatactgga gatgctaaga
    80221 taactagagg ttattgttta cctgcaaaat ttgtagtaca tactgttgga cctattgtat
    80281 ctggtggtca gccaagtaaa gagcaagaaa agcaactgct tcattgttat aaatcatgtt
    80341 taaatacaat aaaagaaatt gatgaaataa aaaatattgt tttttgtgga atttctacag
    80401 gtgtatttgg ttatccaaaa aaagaggcag ctaatcttgc tgtaagtaga gttagactat
    80461 ggctaaaaga aaatcctgaa aaaaatttaa aagttgtatt taatgtgttt actgaagaag
    80521 aagaagagaa atatagaaga atatttaaat aatattatgt aacagaatcc taaaaatact
    80581 tattatataa tacaaattca ttgtataaga taataattta caagtagtaa aaattaataa
    80641 aatataattt gtatttttaa taaataagta ggtataatag aaattataag aatagtataa
    80701 tcaattatgc tattacttag aaaatataat ataggaaaat tgtatggtaa ataaatatga
    80761 aaaatagttt taaaaattgt attagtttaa gaaataaatt tataattata tcaattgtgt
    80821 ttctattaat cctattaatt gtatcttata tatttggaaa attagtgtat aatagttcag
    80881 ttggtttaga gcagttaata aaaaacgaag atatagtaaa aatattttct gagagaaaag
    80941 ataaaccact ggagaaatta aaaaactaca agacatctga attaatgata caaagttcta
    81001 ataattacaa gcttgaaagt ctttttataa cttcaaatat aaagacaaga gatactatga
    81061 tacttgttca tggaattgga agtagttact atgaaatgtt aaaggtagca tatagatatt
    81121 tagataaagg ttataatgta ttagtttata atcaaagaaa tactggtaat agtggtggag
    81181 ataattacac atttggtctt tatgaaaggt atgatttaga tagtctagtt aaatttgtaa
    81241 aaaataaatt tcctgaaggg agattaggtg tgcatggatt ttccatgggg gcagggacag
    81301 ctgctatgca ttcagagata aattcaaaag atgataaggt cgatttttat atactggatt
    81361 ctccttattc tgaaatgaaa gatgcaatta gaatgggagt actagaaaaa cgaataccag
    81421 atatattaat caattatgtg gtgacttgtg gagatttata taataagttt aaatctggat
    81481 tttggtacag tgatgtaaaa ccatatgagt cagttgagaa atctaatgta cccatactgt
    81541 ttatacatgg aacaaaagat actgtttgta attatcaaaa tagcaagaaa atgtatgatt
    81601 tagttaaaca tgataaaaaa gacctatggc ttatagaagg tattggtcat gttgatggat
    81661 ttgaacatga tagtacagta tacttcaata aaatatttaa atttatagat agtaatgttt
    81721 taagtgataa aagttaattg aatattagta gcttatttat ttttgtattt attaaaagat
    81781 agataggcta tttttatgtt taaatataag ttttgtttag taatgtgaat taatttctag
    81841 taaaaatgaa aataactacc aattaaattc atttatacct caaataaaag agttagtatt
    81901 agattacaat caaatctaaa gaaaagagag gtagtatata tgaaaaacaa aaataataaa
    81961 gataaaaaca tgttgaatat taaagtattg agagttctag gagtgctatt aatacttttt
    82021 gattatacgg gtgatacttt gaatttttat aatttcttcg caaagccatt agtatcgggt
    82081 aatttatata agacaacagg tactgactgc attgtatttt tattatttat tgtagagtgt
    82141 gtatacctag tgggaagata tatattaaaa gttaagataa attggagtga gattattgaa
    82201 aatgagaaag gtaattttat aaataacttg ttggttgaaa aaatacaaga agaaagcaac
    82261 attgtatata atttattaaa ctcatatgtg ttgccaaata tatataagaa agcaagggga
    82321 ttgatgttta aaacatatca tatgttaatc tacattaaag aaaaatacta ttatgtaaat
    82381 atatattaat ataatatgat tgcacaatta attatattat attttgtatt ggatgataaa
    82441 atgaaagaca taaatttttt gagacactaa aatcgcaatt tatttcgtgt ttaagttata
    82501 aattttgtga aaaattataa atcttataaa atagattgcg attttaatct taaacttttg
    82561 ctatagtttt tccagtatca gttaaatcat atccgccaag accttgaagt tcagctttaa
    82621 actcaggaga gtttattatg ttaattatag tttggtaaac tggattttgt aagtcttctt
    82681 ttttaataac taaatcatag cgctctttgt gaagaggaat aaagtcgata ttatttactt
    82741 gtaaagcagc tttttcattt cccaaaccaa catctgctat tccacgagaa acagtacttg
    82801 caactccaag atgtgataat tcttcattat cataaccttt tatatgttta ccattaaagt
    82861 ttagaagtct aagtttacca tctagtaaaa ccctagttcc agaacctttt tccctattta
    82921 caatggttat acttggtttt gttaaatcct cccaggttgt tatctcttta ggatttcctt
    82981 tagcaacata gaaaccttgc attctataag ctagatttat aagtacacat ggtattcctg
    83041 gcaatagctt tttaacaaaa gtgctattat attcatccgt ttcgaaatcc catagatggg
    83101 atgatgctat agaaactctg ccattatata actcatacaa agcattataa ctacctatat
    83161 aagagcgaaa tgtacttatt ccatccaatt ttttttcaac agacctagct aaaatgtcta
    83221 atattacatc ttgaccagaa ataataattt tgaaattatt atttatatct atatttttat
    83281 tgttgaaatg tgtatccttt actttaccag ttttttggct gttgatatat tcttcaacat
    83341 ctttagtatc aactcttatt tttttcccta ctttataaga aggaagttca cctcttttga
    83401 ttaattcata tacagtattt tttgttatct ttaataattc agctacctcg acaggtgtca
    83461 aagatgataa tgagtccata atccacctcc atataaggtt attttaacat taaaaaaagg
    83521 aaaatgtagc aaaatattta aaagtagtgt aaataaatta cttgaaaatt tattatcatg
    83581 tatatataat taaactatat ataacaaaac atgataaaac ataataaaac atgttaaaat
    83641 gtgtatcaaa tttacaaaaa gggagattgt tatgaaaaaa atattgggaa tattaggatt
    83701 agttgcatgt ttaactttgg ggacagttgg gtgcaatagt aatgaagaca aaaaagatga
    83761 tggaaaacaa gaaaaaacaa ctaaaagtag tgattcagta gagttaaata tatcggctgc
    83821 tgcaagttta aaagaagcaa tggcaaagat tgaagaagaa tataaaaaag ttgattcaaa
    83881 tgtaaaactt acagttaact atggagcatc aggctcatta caacaacaaa tagaacaagg
    83941 agcaccttgt gatttattta tttcagcagg tcaaaaacaa atgaaagctt tggatgaaga
    84001 gaagttatta gtttctgata ctatgaaaga tttagtaaaa aatgatttag ttcttatttc
    84061 ttctgctgac agtagtgtat caggtatgga agatttgact acagataaag tgaagaaaat
    84121 tgctgttggt gaagctgaaa gtgttccagc tggtaagtat gcagatgagg tgttaactaa
    84181 tttaaattta aaagataagt taaaagataa gctagtattt gcaaaagatg taaaagaagt
    84241 tttagcatgg gtgcaatcag gaaatgctga tgttggattc gtttacttca gtgatactgt
    84301 caataatgat aagataaaag tagttgaaaa aacagacgaa aaaactcatt cgcctattac
    84361 atatccagtt tcagtaatta aagctagtaa aaacgtagat gctgctaaga aatttgaaga
    84421 atttttatta agtgagtcag gacaaaagat ttttgaagaa tttggatata aaaaagtaga
    84481 ataaacttag aaataaatta tgtgtaatta agtataaatt atatataaat aaatgaaaat
    84541 tgaaaataat tgtgtattat aataaaatgg aaatagtgaa attaatagag ttattagttt
    84601 cactatttcc atttaaaatt aaggagggaa agatgggaac agattggtca cccttatgga
    84661 tatcacttaa gacatctatt ttgtcaacaa taataacttt tgtaatagga atatttgtgt
    84721 catatattat ggctaactat agaggtaagt ggaagggtct tatagatgga ctatttacac
    84781 ttccactaat tttgccacca acagttgtag gtttcttttt attattacta tgtgggaaga
    84841 atggatttat aggaaagttt ttattaaatt tcgataggac tttaatattt agttggacag
    84901 caactgtaat ttcagcagta gtggtgtctt ttcctatgat gtatagaact tcaaggtcag
    84961 cttttgaaca gattgataat aatatgttgt cagctgctag aacattaggt cttaatgaat
    85021 ggaaagtatt ttttaagatt gcagttccat tagcatggcc tggaataatt ggtggattgg
    85081 tgctgtcgtt tgccagagct cttggagaat ttggtgctac acttatgata gctggaaata
    85141 tacctggaaa aactcaaact atgccaatag ctatattttt tgctgttgaa ggcggagata
    85201 tgaataaagc tatgatgtgg gttatgatta ttgtagcaat atctatagca atgattttat
    85261 tactaaatta ttggtcggaa tttcagcaga aaattatagg aaagaggtgt ggatagttga
    85321 gcttatacgt ggatattgaa aaagatttat cctcttttaa actaaaggta gaaattaaac
    85381 aagaaaaagg tactctgggg tttttgggag aatctggttc tggaaaaagt atgacattaa
    85441 aatgtatagc agggcttgag aaaccaacaa gaggtaaaat tgtattaaat gatagagttc
    85501 tatttgattc tgaaaagaag ataaatttat caacacaaga tagaaaggta ggatttttat
    85561 ttcaaaacta tgctttgttt ccacatatga ctgtaagtca aaatatagaa cttgggcttt
    85621 taaaactaag taagagtgaa aaaaaggaaa tagtagcaag gtacttagat atacttaaat
    85681 taaatgggtt tgaggggaga tacccttggc aattatctgg tggtcaacaa caaagggtag
    85741 cactagctag agcattggca acatctccag atatattact tttagatgaa cctttctcag
    85801 cattggacca tcatttaaga agcaatatgg aaaaagaact tatgaatatg ttaaaagatt
    85861 acaagggaga tatactattt gttactcatg atattgaaga agcttaccga gtttgtgatg
    85921 atattatagt gtacaacaaa ggagaaggtc ttccaaagag acctaaaaaa gagctctttg
    85981 aatctccaaa gtaccttata gaagctaaga taacagggtg taaaaatata tccaaattaa
    86041 atagactgga taaaaatact atttatgcta cagactgggg atgcgagttg acattaaata
    86101 gagaaatagg tgataatata gaatatgtgg gaatcaggga gcatcatatt aaggtcttgg
    86161 attctaatga agatttaaat gaaaagttat gtttcgaatt gattaacatt gtagaaaatc
    86221 cttttacata tactatatat gttagaaaaa ctgatttatc taacgaatgt gtacctattc
    86281 aaatagaatt ggaaaaaagt aaaatgagat ttaagaaagg tgacagaata tatttagatt
    86341 ttccacagga atatttattt tgctttagat acaattataa caaaaaggag taaactatat
    86401 gactgaaaaa gaaaaaatgt tgtctgggaa aggatactat gcaaatgatg aattgttagt
    86461 aaaagaaaga gagtattgta aaaaattaac tagattgttt aataatactt tagaggatga
    86521 atatgaaaaa agagaagata ttttaagaca actatttggt tcagttggca agcaaataaa
    86581 tgtagaacaa aatattaggt gtgattatgg atataacata catgtaggcg aaaatttctt
    86641 tgcaaattat gattgtattt ttttagatgt atgtaaaatt gaaataggtg acaatgtaat
    86701 gttagctcca aacgttcaaa tctatacagc ctatcatcca attgatgcac aacttagaaa
    86761 ttcaggtata gagtatggtt ctcctgtaaa aataggtgat aatgtatgga taggtggggg
    86821 agtaatcata acaccaggaa ttactatagg tgataatgta gtaataggtg caggaagtgt
    86881 agtcacaaaa gatataccac caaatactgt agctgttgga aatccatgta gagtaataaa
    86941 gaaaatagaa gaataaatat gattgtataa agtttataca agtaaaattt atacaaacaa
    87001 attaagagca tttacatata atctttaaat agataataca ttaaaataaa aatatatgcc
    87061 tgtttctata aattaattta tagaaacagg catattaatc gtatatagaa aatatctttc
    87121 tattcttgtt tagtctttac ataagaaata ttttcattag aaggtttttc catacctaaa
    87181 gcttttaata tatcttcatt aacaatgata tctgtatcat ctgatgtagc cacaggcata
    87241 tcagatactg attcaccatt taaaatttta actgccattt cacctgtttt gtaacctagt
    87301 ttttcgtagt tgataccttg acaagcaaga gcaccttttt caactggccc agattcagct
    87361 gctataacag gtattttatt ctcagtagct attttagaga ctataggcat agaagaagcc
    87421 actaagttat ctgtaggaac atataatacg tctattttgc caactaaact agaaatacct
    87481 tggttaactt catttgaagt agttatacct ttttcaacaa ctttaaatcc atttttagat
    87541 gcatattctt ttaaagcatc aacttggact ttggaattta cttcactagt attgtacata
    87601 actcctatag tttttgcctt aggagcaaat attttaagaa gttctaaacc tttgtctaca
    87661 gaaacaaaat ctgatgttcc agatacattt gtgcctggct tttcaagagt tttaactaat
    87721 ccagcagcaa cagggtcact cacagcagta ataagtatag gtatatcttt agtagcatta
    87781 aatgctgcct gagctgatgg agtagatata gcaaatatta agtccttctt atcagatgca
    87841 aatttactcg caatactttg tgtagtaggc atgtcatttt gagcattttg gaagtctata
    87901 tcaatatttt ctccatcttt aaatccgttc ttttctaatg cttttacaaa acctgtccta
    87961 gtggcatcta atgctggatg ttcaaccagt tgagttatac caattttttt aacttcttta
    88021 cctttagaat tactttcttg ttttgaatta ccagagtctc ctccttgtga acaccctgta
    88081 agcatagata tactaagagc accagctaaa atcaaacttg ccaatctttt tttattaatc
    88141 atttttatat ccccctcaaa aatttattat tatatatgtt catatattca tatgaattat
    88201 tacaaagtaa ccataattat tgtaatttta actttatttt aaagccgagt ttaatacaat
    88261 agatatatta ttttaaactg catattcaaa tttttctaag atttcttcaa ctgttaattg
    88321 ttctttttct ttacctttta catctaatac aacttcaccc ttatgaagca ttatcaatct
    88381 atcaccataa tgaagagcgt gttttaagtt gtgagtaacc ataagtgtag ttatattttt
    88441 ttcccttaca atcttattag tcaattcaat tacttcattt gatgtttttg ggtcaagtgc
    88501 tgctgtatgt tcatcaagta ataaaactga tggatttgtt aagcacgaca taattagtga
    88561 taatgattgt ctttgacctc ctgataaaaa ttttacttga acatcaaggt atttttttaa
    88621 atcaagagat acaccttcca ataaattttc taagaaatct cttttgtaac gaagacattt
    88681 tcttagattt aatagttttc ctttgttaag agctaaagac aagttttctc taacagtcat
    88741 agaagggcaa gttccaagac ttgggtcttg aaaaactcta cttactattt gagttcttct
    88801 gtgttcagct aaattagaca agttatgttt atttaataat acttgaccag aagattcttt
    88861 tataagcccg gatattatat ttaataaagt agatttacca gttccattac tacctatgat
    88921 acttacaaat tcaccatctt ctatatcgat tgataaattt tcaaagatag tattaggttc
    88981 accatatgga ttagggaagc tctttgaaag atttttaatt tgtaacatct acaacacctc
    89041 ttttttctga tttatttttg gtactagctt tgcatttgtt tttttttagt gaaatattta
    89101 agttaccagt agctaggaaa gatattataa ctatggcagt tattagtttt aagtcagttg
    89161 ataacattcc taaactcatt gcaaagtata tagtgaattg gtatataaaa gagccaacaa
    89221 ttattgcagt agtatctttg ataaaagtaa atttcttaaa tagtgtaata ccaattatga
    89281 tagaggcaat tccaagaact aaagttccta tacccatatt tacatctgag aatcccaaga
    89341 actgagccat aagactacca gataaagcta taagtccgtt tgaaatcata agtcctagta
    89401 tttttataga accgatattt atacctaggg atttaatcat ttgagagtta tcaccaacac
    89461 cttttaaagt gtatccaaga cctgttttta ggaataaatc taaaaggact ttacagatga
    89521 atacaaaagc taaggctaaa actataggag atatttcccc attgaataag tgtttaaagc
    89581 taaatagagg aatatttgat tttcccatta ttcttaaatt gatagagtat agcattccca
    89641 taactaaaat accagaaagt aagttagata tcttaaactt aatgtgaaga atccctgtaa
    89701 caagaccagc agtacaacca cataagatag ccattaaagt tgctactaca ggagaaatac
    89761 cattagtaag agaaaatgct acaatagaag cacccattgt gtagctacca tcagcagaca
    89821 tatctggaaa atctaagatt ttgtaagtta tataaacccc cagtgccata atcgataaaa
    89881 ttaaactttg cgtcattaca gatataatac ctgacataaa taaaacactc ctttgaaaat
    89941 atttttataa ttttaattat ttgctaattt tgtgtataaa aaaaccacat agtgtacaaa
    90001 actatgtggt aaaaacacga ataagccaca tagtttaatc tatgtggctc ttatatagtt
    90061 aaatgaaaac tttataaaca atatagccaa catagataca atcccaaaat tttagtagtc
    90121 ttaaggggtc tgtatctatg ttaaaacgta ggtacaaacc ctaaaaatag tggctataat
    90181 aataataata ttttctattg tttaataaag tattcataat tatctctccc ttttaatatt
    90241 ttaattagtc agaaaattat atgtattctc gacttgttat aaattataaa ttatacaata
    90301 ataaaagtca actgctaaat ttaattaaaa tactaaataa tgtatactca gaatactttt
    90361 aaattaatgt attgtttgaa aaaaacgtta tcctaagtta taagtgaatg tggaaatttt
    90421 atcaaaatat ttactaaata tggaataact agacatgtat atcaatcaaa tatcataaaa
    90481 aaaagctgtc tggagaatat taaaattctt ttggacagct caagtagtga ttagaaagtt
    90541 atataacttt cataatcagt cgtcaatata gacgactttt atggaaaatg aataacttta
    90601 tatgcaatat tcaaattgct acaataagca caattttcaa agggttaggg tggataagag
    90661 tacttattat ataaagtttt catattacct acttcatatt acctattttt tgaaattaat
    90721 aagttttatt ttgtagattt tgcattttca tctgacttta tatactttat attatctgct
    90781 gataactttt gcatgtcaag tgcttttagt gtatcttcgt taattattat ttcagtttca
    90841 tctaatgtag taactggtat atcgccaaca gattttcctt ctaatacttc aatagcaagt
    90901 tctccagcct tataacctag tttttcataa tctattccac aacaagctaa agcaccagaa
    90961 gatacagaac cttcttcaga ggctattata ggaatcttgt tttcattagc aactttagaa
    91021 actattggca tagaagaaac tattaaattg tcagtaggga catataaaac atctatttta
    91081 ccaactaaac tagaaatagc ttggttaact tcacttgaag agctgattcc tttttcaact
    91141 acttcataat tatttttctt agcataatca tgtagagaat caacttggat ttttgaatta
    91201 acttcactag tattgtatat aacccctatt ttctttgctt ttggagtcaa tgttttaacc
    91261 aattctaatg ttttatcaat tgaaagataa tcagatgtac cagaaacatt tccacctggt
    91321 ttttcaagag atttaacaag tccagcttct acagggtctg taacagcagt cattattatt
    91381 ggaatatctt tagtagcatt ataagctgct tgtgcagatg gagtagatat agcatatatc
    91441 aaatcttttt tgtcggatac aaacttacta gcaatacttt gtgtagtagg catatcattt
    91501 tgtgcatttt ggaaatctat ttttatatta tctccatctt tatagccttt atcttcaagt
    91561 gctttgatga atcctttctt tgctttatcc agagatggat gttccactaa ttgagtaata
    91621 cctatatttt tttgtttttt gctatctgtt tctttatttt catttgaagc attggagcca
    91681 tcattttgag aacatccagt aagcattgat acaccaagta ctcctgctat aattaaactt
    91741 aatatttttt tactttttat cattttattt tcctccctta aaatttaata attatatttg
    91801 ttcatatatt catatgatag attataaata tttctgatta aaactaagat tttaatttct
    91861 tatactgcat attcaaattt ttctaagatt tcttcaactg ttaaatcttt tttttcttta
    91921 tcttttacat ctaatacaac ttcaccctta tgaagcatta tcaacctatc tccatattga
    91981 agagcgtgtt tcaagttgtg agtaaccata agtgtagtta tatttttctc tcttacgatt
    92041 ttatctgtta actctattac ttcatttgat gtttttgggt caagtgctgc tgtatgttcg
    92101 tctagtaata agaccttagg acttgctaag ctagacataa ttagtgatag tgattgtctt
    92161 tgaccccctg aaagatattg tactttaata tctaaatatt tttttaaatc caaagaaatt
    92221 ccttcaagta agtgttccaa gtcattcgtt ttatgacgaa gacatttttt taagttcaat
    92281 aatttccctt tattaagtgc tagagataaa ttttctctaa ccgtcataga aggacaagtt
    92341 ccaaggcttg ggtcttgaaa aactctactt actatttgag ttcttttata ctcagctaaa
    92401 tttgttatag tagtactatc taaagtaatt tgaccagaag attcttttat aagcccagat
    92461 attatattta ataaagtaga tttaccagtt ccattactac ctatgatact tacaaattca
    92521 ccatcttcta tatcgattga taaattttca aagatagtat taggttcacc atatggatta
    92581 gggaaactct ttgaaagatt tttaatttgt aacatctaca acacctcttt tttctgattt
    92641 atttttggta ctagctttgc atttgttttt ttttagtgaa atatttaagt taccagtagc
    92701 taggaaagct attataacta tggcagttat tagttttaag tcagttgata acattcctaa
    92761 actcattgca aagtatatag tgaattggta tataaaagag ccaacaatta ttgcagtagt
    92821 atctttgata aaagtaaatt tcttaaatag tgtaatacca attatgatag aggcaattcc
    92881 aagaactaaa gttcctatac ccatatttac atctgagaat cccaagaact gagccataag
    92941 actaccagat aaagctataa gtccatttga aatcataagt cctagtattt ttatagaacc
    93001 tatatttata cctagagatt taatcatttg agagttatca ccaacacctt ttaaagtgta
    93061 tccaagacct gtttttagga ataaatctaa aaggacttta cagatgaata caaaagctaa
    93121 ggctaaaact ataggagata tttccccatt gaataagtgt ttaaagctaa atagaggaat
    93181 atttgatttt cccattattc ttaaattgat agagtatagc attcccataa ctaaaatacc
    93241 agaaagtaaa ttagatatct taaacttaat gtgaagaatc cctgtaacaa gaccagcagt
    93301 acaaccacat aagatagcca ttaaagttgc tactacagga gaaataccat tagtaagaga
    93361 aaatgctaca atagaagcac ccattgtata gctaccatca gcagacatat ctggaaaatc
    93421 taagattttg taagttatat aaacccccag tgccataatc gataaaatta aactttgcgt
    93481 cattacagat ataatacctg acataaataa aacacccctt tgaaaatatt ttaaatggtt
    93541 tttaaaattt ggttttaaaa aatgattttg atataaaaaa accacacagt atatataact
    93601 gtatggcaaa aattcgaata agccacacag tataatccat gtggctctat attatgatga
    93661 taaaataaaa actttaacaa caattactat agccaacata gatacaatcc ttaaaaatca
    93721 taatcctaag gggcttgtat ctatgttatg acaatttgtc ttaacttagg tacaaaccct
    93781 aactaaattg gctactaaaa aatcgtactt gtttgtttaa taaagtcttc atatttacct
    93841 ctccttttaa tttaataatt ttgaaaattt tgttttctgt tttgtatagt aattagttta
    93901 atacagtaaa aaataaaagt caactataaa ttttgaaaaa ttatcaaatt atcagaaaat
    93961 tgagtttaaa taaaaaaatt tttacatgta gaatatagtt atattcttaa ttattgtttt
    94021 tttataaaaa tgaaaaatat aaataagtca taaaagatta taaaaataaa gagatattta
    94081 gataattagt gtaaataggt tttaaatcaa cataataact taaattatat ggtataataa
    94141 attaaataat tgttttaatt caaaattttt tgttatttta aattatttca aaactcttag
    94201 ttttagtgat tttatttatg atatttatgg gattaaaaag atttcaatag atatttttgg
    94261 taagtattta agaagtcttt aactttatag gagtgataat ttatgctaat aggtacatct
    94321 aaattaagca gaaatgttga tactgcatgt gtagaagaag tagggattcc aatgattgta
    94381 ttgatggaaa atgcggttat atctgcaatg aaaaatatgg atatagacat gtataatagt
    94441 tatacagttg tatgtggtgt tggaaataat ggaggagatg gtcttggaat agcaagacat
    94501 ctaaatacat atggaaaaga agtaaacgta tttttagtag gtaatcttga aaaactaagt
    94561 gaatgctcta aaataaatta caatgtactt ttaaatatgg gaattaatat tataaatatt
    94621 aataaaaatg atttaagtga aaaaaattat ggagaggaaa atgaagtaga taaaattatt
    94681 aaagttgaga aacaaaatat tataaaaaat aaaaatttaa caatttttaa agatagagta
    94741 tctaaaagtg atgttgtagt agatgctata tttgggactg gattaaaacg tgatataact
    94801 ggtattttta aagaagttat tgacatagta aacgataata gcaaaaatac gtattctata
    94861 gatattcctt caggtataga tagcgataat ggaaatgtat taggaacttg tattagagca
    94921 aacaaaacga ttagctttga gttttataaa agaggttttc taaactatga cactagcgca
    94981 cttataggtg atgtagttgt agaaaagata ggtgttccag attttattac aagaaaatac
    95041 catgatgaag agttcataac tgataagact tttgtaaaaa ataatattag aagaagagat
    95101 aagcatggtt ttaaaagtga ttttggaaaa gtatctatag ttgcaggttc aaaaggattt
    95161 tatggtgcaa gtttcatagc aactgaatct gctgttaaga gtgggagtgg acttgtaaca
    95221 ttaattagta gtgaagatgt gcaagaaaag gtatctatta gacttacaga agcaatgact
    95281 ttaaattttg aagaagaaag attaaataaa ttacttaatt ctagtgatgc aattggtttt
    95341 ggtccaggta tgggagataa tagtcaaaca tttgataaat tacttaagat agtagaaaat
    95401 tctaattgcc caatagtatt agatgcagat ggattgaacg ttatgaagga cagatgttat
    95461 aagtttttag aatggaaaaa tagatttgta attacacctc atttgggaga aatggccagg
    95521 ttgacaggag attcaattgg gtatataaga gaacatagag tagatgttgc aaaagaattt
    95581 gctcaaaaat ataatctggt ggtgctttta aaaggttatc aaactgtcat aacagatggt
    95641 aaaaaaacat atataaatcc tacaggaaat agctgtatgg caactggtgg aatgggtgat
    95701 tgtttgcttg gaatgattac ttcatttata ggtcaaggta tggatatatt ggaagctaca
    95761 gttagtggag catatataca tggctatata ggagataaac tgagcaaagc aatgtatacg
    95821 gtaaatgcta cggatttaat aagtaacatt tcattaacaa tgaaagagtt gttggatata
    95881 taattggtga tatttgatac tactttgtaa caaatttaac aaagtatgtt gatataatac
    95941 tattaattaa ttaattaatt tattttattg ttttgtaatt taaatttata ttcaaaaaca
    96001 ttgtataaac atataattta tatagggttt tataattcat atgaaaagag ggtagatagt
    96061 atgaagttaa tatggttgat agtagcagtc ttatttggaa tagcagaaat gttaacacca
    96121 agcttaacat tgatatggtt tagtgtagga gctgtaatac ttatattttt aagtagcttt
    96181 atagagagta ttttcttaca aattttaata tttgcagtaa tttctatagc aatgttagtc
    96241 gtagctacta aaaaaatagt aaaaaaagat aaaggctata aatctaacac taatttacaa
    96301 gcaatgatgt ctaaaaaagg tattgttaca gaagagattt ctccaaataa cacaggtcta
    96361 gttgtagttg aacatgaaac atggacggct atttctatag atggtgaaaa gatagaaaaa
    96421 ggttctacag tagaagtatt aaaaattgaa ggggtaaaat tagttgttaa aaaagtagac
    96481 gccacagtta atgtgacaaa ccaatagaat aaaggaggat ttatagtatg ggtactaaaa
    96541 ttgttttaag tattgtatta atagtagtag tcgtagcaat aagtttaact tgtataagag
    96601 ttataaaaca atctaaagta ggtataataa tgagacttgg taagtttcaa aaagtagctg
    96661 aaacaggtgt acatttctta ataccatttt tagataaaat ggcatatgta attgacctta
    96721 gagaaattgt aatagatttt ccacctcaac cagttataac taaagataat gtaactatgc
    96781 agattgatac agttgtatat tataaagtta cagacccagt tagatatgtg tttgagatag
    96841 ctaatccaat agctgctatt gaaaatttaa cagctacaac actaagaaat ataattggtg
    96901 aacttgattt agatgaaaca ttaacatcaa gagatataat aaatgtaaaa atgagaacta
    96961 tccttgatga agcaacagat aaatggggta taaaagtaaa tagagtagag ttaaaaaata
    97021 taatgcctcc tcaggatatt caagttgcaa tggaaaaaca aatgagagca gaaagagaaa
    97081 gaagagaggc aatacttcaa gcagaaggta ataagtcagc tgcaatactt caagcagaag
    97141 gtgaaaaaca atctgcaata ttaacagcag aagccaaaaa agaagctatg gtacgtgtag
    97201 cagaaggtga aaaagaatct gctatattag tggcagaagg tgaagctgaa gctataagac
    97261 agacagctat tgctaaggca caaggtgaag ctgaaatgat aaaaagaact caaatggcaa
    97321 cagcagaagg tttaaaatta gttttttcag caatgaaaga ggctgatata gacaataata
    97381 ttctagcatt aaaatctatg gaagctcttg aaaaaatggc tgaaggtaag tcaacaaaac
    97441 tggttttacc ttcagaagca gttaatttcc taggaacatt caaaggaata aaggaagtta
    97501 tgagtgatga taacaaagaa gtacttgata taaaagaagt tttaaatgat aatgaatcat
    97561 taaaaaaata aaagagtttt gttatcatag gaaggatatg gaaattaaag gtcgaattaa
    97621 ttaaatatag cacaaaaatg cacgttgaaa agaagaggac ttataaattt ttaagtcttc
    97681 ttctttgttt ttattagaaa gatgaaatta taaaaataaa ttaacagaat ctttatacaa
    97741 tttatccttt actaatgtaa aaagtgttat ataatataat ataaaacata tagcaaataa
    97801 gcatggtttt agttagtcta tttatattat taatatatat ttataaaaat ataaacatta
    97861 atactaagaa aactaataaa taatttaaaa gtttatatcc ttttgtttaa attatagaca
    97921 aagaa
//
LOCUS       DAEPIN010000007        90915 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.007, whole genome shotgun sequence.
ACCESSION   DAEPIN010000007 DAEPIN010000000
VERSION     DAEPIN010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 90915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 90915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..90915
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.007"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            <1..1548
                     /locus_tag="KR250_000612"
     CDS             <1..1548
                     /locus_tag="KR250_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBH0066581.1"
                     /translation="SIVTKVQEFGKLRAIGATKKQIKNIVFKEGFILAGIAIPIGIIL
                     GYVLANIIIKSFMDIDVKSSRLPVILSVVVISFISVVLSLLKPMKVASKVSIVDAVRY
                     TGNKISNKSKRKGYKNINLKRLSHANLERNKKRTYMTLASLILSGTIFITVSTALESF
                     DAEKMAREHFPYDIEVRLSGYEMNSDKNPKDNLNILQMDNPLSKDFFNQIKNMEGIKR
                     IESARSVKIGMEDYDVEFKYDLLQSINENDVKSLNKNIIDGKINLERLQTGDEIVITH
                     VDTAKEMGVKAGDKIRLTLYDGDKKIKKEFKVQAIAMGVPSFGIGKDFIDRTLKYDST
                     SSLGIYTEEGKYQEIKDSIKKIAKNNGFLETDFIDSRIESNKATISFIKIMGYTLTGI
                     IGVIGFMNLVNTMITSIVTRKKELGMLQAIGLTNKQLVKMLNSEAIYYTSGMMIGSIL
                     FGGILGYIAVIFLKKTGLSYATYSLPIVPILLMIVCILIAQFITTYLIGRSFNKESLI
                     DRVRYSE"
     gene            1828..2970
                     /locus_tag="KR250_000613"
     CDS             1828..2970
                     /locus_tag="KR250_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HBH0066582.1"
                     /translation="MNKKINYAPGPTETRENVRLARAEKTTNPDIDIDFVEFYKKTCE
                     KFGNIIGTKNDVYILSGEGILGLEAACASLTEKGDRVLVIDNGIYGEGFKDFVTMYGG
                     EYVLFSSEYTKSIDIDELKAFLDEDSNFKYATVVHCDTPTGVLNDVSKICPLLKKYGI
                     LTVVDSVAGMVGERLSVDESKIDIILGGSQKAISAPAGLTIVGISQDAKNCIKNRKTD
                     VIGFYCNLNIWEGYYEKKYFPYTMPISDIMGLDKALDNILEEGIENVLNRHEKIAYSV
                     RKSIEEYGLELFLEEGYSNTVTAIKIPESIGALELTDYMLKNYNTLVATSLNQYMDTI
                     LRIGHMGENANLNKIEHVLNVLDKSLSALGFKGNGNLLNLFNKYYF"
     gene            2989..3513
                     /locus_tag="KR250_000614"
     CDS             2989..3513
                     /locus_tag="KR250_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBH0066583.1"
                     /translation="MEVTAKQKFTTKDMVETSLLIALVFIATKFINIRLPISINGGLV
                     HLGTAMLFISAIVFGGKKGALSGAIGMSLFDLISGWTLWAPFTFIVRGVMGYLLGKIA
                     WANGNNGNNFLINVIGICVSSIWMLFGYYVTEVILYGNFIVPLTSIPGNLMQVLIGLI
                     IALPISKVLKKCIK"
     gene            3613..4431
                     /locus_tag="KR250_000615"
     CDS             3613..4431
                     /locus_tag="KR250_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBH0066584.1"
                     /translation="MLSVTRYSIKNVRLKPWVKFIWYFETKSNILLNNKLLPTDSIDI
                     ILNLSDVMEYKIENQDYTASNMHFNGIRDKHGFIIQHGNIRVIGISFYPFGLYPFLKI
                     PISEFNRQIVDLEAVSQLFAKKLEESLNPTQSVEKIVLYLEEVLLSILEEDLISNKYV
                     KLLNSFIYVNRYSNIKAFCDDTNINIKTLERICLKYTGYTPKILKRIYRFKMASNQLI
                     YNYKDDELFDFIYENEYYDQAHFIKEFKKFSGTSPIKFIGENKTIKENIKYSYL"
     gene            4509..4820
                     /locus_tag="KR250_000616"
     CDS             4509..4820
                     /locus_tag="KR250_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HBH0066585.1"
                     /translation="MKIITEIVEFNVEENLSKEEFIEIVNDLELKFHSIQSGFIDTEL
                     LYDEKSQKWIMIQHWESIEDLKSASSKMFKDSSTEKFRKALKNQTVKMNIIPQIKSWK
                     L"
     gene            complement(4947..6950)
                     /locus_tag="KR250_000617"
     CDS             complement(4947..6950)
                     /locus_tag="KR250_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HBH0066586.1"
                     /translation="MKSQKRFKILDIDVYLQPFESDIKKRMKHYENMKSKTLKNYKDF
                     SSFANAHLYYGFHQTNDGFFYREWAPNADSLSLIGDFNNWNRKSHPLRKIGSGNWEIF
                     IPGQNSLPHKSEVKVQVTANGKTFDRIPLYIKRVIQKDFGGFNGQIWQPKTPFIWTDN
                     DFDLKNITYPLIYECHIGMSTESESIGTYNEFTEKILPKIKKAGYNTIQLMAIMEHPY
                     YASFGYQVSNFYAISSRFGTPEDLKNLINTAHSMGIAVLLDLVHSHAVKNTLEGINEF
                     DGSEHQFFHSGSKGNHPAWGTKLFNYGKPEVIHFLLSNIKFWLNEYHFDGFRFDGVTS
                     MLYHNHGLGVSFDSYEKYFSMNTDIEAITYLQFANELIKEIKPNSISIAEDMSGMPGM
                     CIPIKDGGIGFDYRLAMGVPDFWIKTISNLSDEDWDLGKMWYELTTRRPGEKNIGYCE
                     SHDQALVGDKTIIFWLADKEMYWNMEINSNNHVINRAISLIKLIKLITFSLAGEGYLN
                     FMGNEFGHPEWIDFPREGNNWSYKYARRQWSLSEDNNLKYKQLLNFDKSMLELTKYSD
                     IFIQNSHELIHMDNDKKILVYSKGKYLFIFNFHPNLIQSVDISKFQNTNYKTILNTDM
                     VEFGGNTKKENLRDYDVYFSSTPPSSKMPIASRTAIVLLDSSI"
     gene            complement(7103..7741)
                     /gene="recX"
                     /locus_tag="KR250_000618"
     CDS             complement(7103..7741)
                     /gene="recX"
                     /locus_tag="KR250_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HBH0066587.1"
                     /translation="MGIITKIEQQKRNDDRVNIYVDDKFFIAIFKELVYTFNLKKGNE
                     INEEELKPILDDEMYIKAKNKALNILSHADQSEKKLKEKLSSEFDENIIERVIDFLKS
                     YNLVNDSVLAQKIVNTNVNLNKCGKNRIKQNLYNKGINRSTINEAVSELDKDTEFENA
                     MYLAKKRYERVKKEDKKKIYQKISQHLSYKGFDYDIIKRVLNKLLNFDEYDI"
     gene            8017..8706
                     /locus_tag="KR250_000619"
     CDS             8017..8706
                     /locus_tag="KR250_000619"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HBH0066588.1"
                     /translation="MRSENLVKMAELKNTEKLEKFNRHKGKIKIGILGGTFDPIHYAH
                     LATAEFIRDKYDIDKIIFIPSGNPPHKLCITTDKYDRYNMTLLATESNEDFLVSKVEI
                     ERKKRTYTIDTLKYLKKKYKNADIYFITGADAICSVEEWKDVKKNFELATFIAATRPG
                     ISLLRSQETIEKLTKKYNADIITVYVPSLDISSTYIREQLNEGKSIRYLVPENVENYL
                     YENKLYQYGDD"
     gene            8707..9279
                     /gene="yqeK"
                     /locus_tag="KR250_000620"
     CDS             8707..9279
                     /gene="yqeK"
                     /locus_tag="KR250_000620"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HBH0066589.1"
                     /translation="MNLENINQRLNQMLPVGRLSHSKNVAKCAEKLCEIYGCDKEKAY
                     LAGMIHDCAKYLSDKEIEDYVNKYEIYLDPIEDGNRSLSHSVIGAYICEYEFEVEDED
                     IINAIKYHTTGREDMSLLEKIIYIADLIEEGRKFPVVDTLRELAYGGKLDEALLTSFN
                     NTLMFVINKKEEIHPRTVMARNYLIKEKLL"
     gene            9396..9746
                     /gene="rsfS"
                     /locus_tag="KR250_000621"
     CDS             9396..9746
                     /gene="rsfS"
                     /locus_tag="KR250_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HBH0066590.1"
                     /translation="MTVEQMTKIAYDAIEDKLGQDTVIINIGKVSSLCDYFIITTASS
                     QRQVKAIADNVEDELAKLGLEPRGKEGQGTQTWVLLDYGDIMVHVFNEENRGFYNLEK
                     LWKDAPYIDIDTLA"
     gene            10100..12520
                     /locus_tag="KR250_000622"
     CDS             10100..12520
                     /locus_tag="KR250_000622"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HBH0066591.1"
                     /translation="MSVYNFKEVESKWQKIWKDNNQYKMDTAQTEKPNYYTLEMFPYP
                     SGKIHMGHVRNYSIGDVVARFKKMEGYNVLHPMGWDSFGLPAENAAIKHGIHPHKWTM
                     ENIEEMKEQLNLLGISYDWDKEVATSTPEYYRFTQEIFLKFLEHGLAYKKKSYVNWCP
                     SCETVLANEQVVQGACERCKATVLKKDLEQWYFKTTEFAEELLNDLDTLDGWPEKVKT
                     MQKNWIGKSTGADLVFDIDGTDKSMTVFTTRPDTTYGVTYMVLAPEHELVKELVAGTE
                     YEADVEAFVQKMHTMTEIERTAADVEKEGMFIGRYVINPLNGKKVPLWIANYVLVEYG
                     TGAIMAVPAHDERDRDFAEKYNLDIIDVITEDNKMINSEEFNGLDASEGFEGIIDKLE
                     KEGRGKRTINYRLRDWLVSRQRYWGCPIPVVYCDECGIVPVKKEDLPVLLPTDVEFTG
                     KGESPLTTSKQFMSTTCPHCGKPARREVDTMDTFVDSSWYFLRYVDSNNENEPFSKEL
                     VNRWHPVDQYIGGVEHAIMHLLYARWFVKAFKSMGMVDFNEPFKNLLTQGMVLMDGSK
                     MSKSKGNTVSPMDIIDEYGADTARLFVLFAAPPERDLDWSEQGVDGCFRFLNRVYRLV
                     DELADVVKKDVEFGELNSQDKDMRYTIHSTLKKVTADLSEKFGFNTAISALMELINDM
                     YKYKELDNINEAVIKEGVQTIVTIIAPFAPHLGEELWTMIGKEGSVFDIDWPKYDEKA
                     LVKDEIEVVVQVNGKVRGKLTVNSNISKDEMEKVALEDEKIKGLVEGKTIVKVVAVPK
                     KLVNIVVK"
     gene            13356..14024
                     /locus_tag="KR250_000623"
     CDS             13356..14024
                     /locus_tag="KR250_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBH0066592.1"
                     /translation="METVTNNQSGVEIQLSLFEAIKDLLLINPIFLLGIILIGIFLVV
                     IFKKNSDIPKVKTSILSLAMYYYLCLTLSHIVGIPTFGEYIRLSQLGETFFNPNINLI
                     PFSDGFSLSFILNILLFVPLGFLCPLISKTFERLRNTFLIGFGLSLFIETIQLFTLHR
                     ATDIDDLLTNVIGTIIGYFCFKLIAKLRIVKLHSDDDSMEQDYTVCIPIAIIIVALIL
                     GFFS"
     gene            complement(14342..14614)
                     /locus_tag="KR250_000624"
     CDS             complement(14342..14614)
                     /locus_tag="KR250_000624"
                     /inference="COORDINATES: protein motif:HMM:NF012798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBH0066593.1"
                     /translation="MSKHQFKSVSTFFTKKNVSQFYKKNNLKKIRIHDLRYTSATLLL
                     LGGTNMKVVSERLGHTDIKITMNRYSHVLKEMDKEASDNLSELLFK"
     gene            complement(14845..15266)
                     /locus_tag="KR250_000625"
                     /pseudo
     CDS             complement(14845..15266)
                     /locus_tag="KR250_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431042.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(15285..15383)
                     /locus_tag="KR250_000626"
                     /pseudo
     CDS             complement(15285..15383)
                     /locus_tag="KR250_000626"
                     /inference="COORDINATES: protein motif:HMM:NF026008.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
     gene            15666..17036
                     /locus_tag="KR250_000627"
     CDS             15666..17036
                     /locus_tag="KR250_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp29"
                     /protein_id="HBH0066594.1"
                     /translation="MKLKKLLSLGLAVSMFAAGPISANALDTIYTIQGKDKYETAALI
                     ADEQNYTTAILINADSTMADGLSASGLAGATNAPIFLTKKNNIPNATLKRVEKAKKVY
                     IIGGESSIDKATETLLKDKGIEAKRLQGSDRIKTSYNVAKEINSINKVNKVILTNAFK
                     GEPDAMSAAPVAVRDKAAIVLTDGKSVPFNTTGVESYVIGGTSSMSDKLVNDTNSTRL
                     GGVDRYDTNKKIVNKFYNGAKEFYIASGTDLVYALVASPMAKNNPIVLVDIGSNKDIL
                     KNAAKVTGIGNLSDKIMLECENTINNISLPSSNKNIICGLKIDFDSKHIIWQDKGSYL
                     ECYDDNLEYYTEKYKDPTVPINIENTSTQNLDVTKLKVYGEANSTLYMGQYAKIKMDF
                     NSFGVLKPGEKCTIYLDIISLDPSRSMTLTFSYVGDKENYKYEQGLASRILFYNPDGE
                     DFFEYK"
     gene            17395..17553
                     /locus_tag="KR250_000628"
     CDS             17395..17553
                     /locus_tag="KR250_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0066595.1"
                     /translation="MDNFLQGILASLSASLIVYIASKLFRKRKKPLKAATKSGWEFDL
                     KIRFHKTK"
     repeat_region   17839..18196
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=17839..17867
                     /rpt_unit_seq="gttttatattaactatatggaatgtaaat"
     regulatory      complement(18338..18435)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            18661..19044
                     /locus_tag="KR250_000629"
                     /pseudo
     CDS             18661..19044
                     /locus_tag="KR250_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431032.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
     gene            complement(19233..20228)
                     /locus_tag="KR250_000630"
     CDS             complement(19233..20228)
                     /locus_tag="KR250_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HBH0066596.1"
                     /translation="MSIKKKVAIVFTGGTISMTVDDKVGAAIPTLSGEQILSMVTNID
                     KVADVEVFNFDEIPGPHITPYRMMELKNYVNDLLARKDITGVVITHGTDSLEETAYFL
                     DLTIDNIKPVIVTGAMRSSSELGYDGSSNLSASVCTAVSKDAMGKGVLVVLNNEVLLA
                     SEATKTNTLSLNTFKSLTSGPLGIIDCNELVLTRDIVNRTIIDTDKVESKVALFKAYV
                     GENADFIRFAVDSGYKGIVIEAMGRGNIPPQMLSGVEYAREKGLPVVIVSRCHSGRVF
                     DSYGYFGSGRDLKNLGCIFGGDLPGQKARIKLILALGKTDNLDEIKDFFEKGIYC"
     gene            complement(20349..21977)
                     /gene="aspD"
                     /locus_tag="KR250_000631"
     CDS             complement(20349..21977)
                     /gene="aspD"
                     /locus_tag="KR250_000631"
                     /EC_number="4.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 4-decarboxylase"
                     /protein_id="HBH0066597.1"
                     /translation="MKNHTYKEIKNIYGKISPFEFKDKLIDIAKYTAKENNRELLDAG
                     RGNPNWTCSTAREAFFTFGHFAITETRSNWDLGHLAGMPQKKGIKERFFKFINENIDM
                     PGAYLARDIINFGINELGFDGDEFVHELADGIIGDNYPLPDRMLPHMEKIVHDYLVQE
                     MKYDISGKYGDVEIFAVEGATAAMCYIFDSLMANELLKKGDTIALMTPIFTPYLEIPN
                     LPRYDFKVVNINANEVDEKGAHTWQYTKKELEKLRDKSIKALFVVNPNNPASIAMDET
                     SCNNLIDVIENYNKDLMIISDDVYGTFVEDFSSLMSKLPYNTVGVYSYSKYFGVTGWR
                     LGTFALHKKNVFDKKINDLTGELKKSVDKRYSDMSLNPSSLSFMERVVADSRLVALNH
                     TAGLSTPQQVQMAFFSAFALIDKVDAYKKLNMSICHRRQKLLFEALELPINENKNNAA
                     YYTQFDIEEWAKLNYGEDLFKFISKTASPVDVLYKLANDYSVVLLSGNGFYGPEWSIR
                     ISLANLYDEAYTKIGKAIREILNGYIVEWQKIKK"
     gene            22636..23853
                     /locus_tag="KR250_000632"
     CDS             22636..23853
                     /locus_tag="KR250_000632"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HBH0066598.1"
                     /translation="MMNVTLTDVKEARETIKNIVKRTDLLESVKLSEKTGANVFYKCE
                     NLQKTGSFKLRGACNKIASLTDEEKANGVIASSAGNHAQGVALGAKMTGIKSTIVMPA
                     TAPLAKVSATKGYGAEVVLNGAVYDDAYAKAVEIQKETGATFLHPFNDKYVIAGQGTI
                     SLEIFEQLDNKVDTILCPVGGGGIISGVAVAAKALNPNVKIVGVQTANIPSMKESIKN
                     GKVTTAFNDTTIADGIAVKTPGDLTFEIINELVDEIVVVEETEIAESILFMMESQKIV
                     SEGAGAVCTAAILSGKYVPAKDENVVCIISGGNIDINTLYRIIGVALAKEGRRYSFST
                     IMEDKPGNFAELTRIISENGGNILSANQGKLSAGEALGKQSAEFILETIDYDHIARIK
                     KAIEEKGFKIIEL"
     gene            23948..24328
                     /locus_tag="KR250_000633"
     CDS             23948..24328
                     /locus_tag="KR250_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HBH0066599.1"
                     /translation="MKHEVIHTNDAPAALGPYSQAIKAGNLLFVSGQVPLVPETMEVV
                     EGDVQAQTAQSLKNLKAILAESGADFSNVVKTTVFIKDMNEFGAINEVYAEYFGENKP
                     ARACVEVARLPKDVKVEIEVIAVL"
     gene            24612..25094
                     /locus_tag="KR250_000634"
     CDS             24612..25094
                     /locus_tag="KR250_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBH0066600.1"
                     /translation="MLNFFKKNKSYKLHAVVSGNSINIEKVNDSVFSKKLMGDGVAII
                     PNSNVVVAPCNGKVTVLTESKHAFGMVSDEGVEILVHIGIDTVSLQGEGFKNEVSQGD
                     TVKKGSPIISFEREKINSQGIDCTTIIIVLNHSEFSEINCMVENEVVAGQDTVIEIMK
                     "
     gene            25115..25930
                     /locus_tag="KR250_000635"
     CDS             25115..25930
                     /locus_tag="KR250_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBH0066601.1"
                     /translation="MRVVKIFNNNALSTVTNDRKEAILLGLGIGFNKRPGDKVNEDKI
                     EKIYYVQDHMQTKFLELLKNVSPEVMDASQQIISLIPGNEGKFNNKGILSLVEHISFA
                     IDRMKNNVFLPNLMLLDIKMMYSKEFELGVKALEIIYRVCHIHLPEDEAGYIALHFVN
                     LQSNDNLAYDTLKFVKGSIDLIKECYGLELDESSLSTLRFRTHLKFLAQRIFKNEICQ
                     DDKMIEMYDYLINNHPKNKEYLEKLNVYIEKEFRYKLDKPEKIYLLLHLTKIL"
     gene            25983..27533
                     /locus_tag="KR250_000636"
     CDS             25983..27533
                     /locus_tag="KR250_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBH0066602.1"
                     /translation="MKEKLLGGLETFAKAMVQPLMYLSSAGILMVVGVLLTNDMLLGV
                     LPFFKWEPIQIFGSLIYNCIMAIINNLAVIFAIGIPAALAKKEKHKAALIGFMSYIIY
                     LTASNTMLTSLGQLAKESKMLGLIGTGQATILGIQVVDTSVFGGIILGYIAGYVFNRT
                     SNKTFKGALQIYSGANFSFICMFFVSILIGVVSNFIWPLAQQGIASLANVIKDTGTFG
                     LFLYGFLERILVPTGLHHLIYTPFQFSDLGGVLKVGADTVSGAYAIVMTEMNLPVERF
                     SDSIYYMATGFTKMFGYIGIGAAFIYTAYKQNKKKVKATIIPLIITAVLASVTEPLDF
                     MFVFCAPLLFFVHAVIAGLFIALLKIFDVTALCGGNLLVSVIMNTTVGVEKTHWPVMM
                     ILGLAQIIVYFVVFSFLIKKFNYKTPGREDESTATGEEQSKQLNLDAGIENIIDGLGG
                     KENINTVENCITRLRVNVKDESKINEDIINLTPNSGIVRKGKDIQIIFGLHVHEVRRA
                     VEDFLEAY"
     gene            27630..28982
                     /locus_tag="KR250_000637"
     CDS             27630..28982
                     /locus_tag="KR250_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HBH0066603.1"
                     /translation="MIITIVGGGSTFTPGIVKSIALREKELELEEIRLFDIDKKRQDK
                     VAVVVNWILKEDVKTDVKLTVTNCEKEAYTDATFIFAQMRVGKYEMREQDEKIPLKYG
                     CVGQETCGCGGMAYGLRTIQPMIKVIDDVEKYAKKDYWILNYSNPAAIVSEACRKLRP
                     NARIINICDMPIAIIDMIAGSLNINDVHNIRYDYFGLNHFGWFTSIDYKHRDLMEEIK
                     EYIKENKILLPKSYIENLNKSRSTGNRHAVSSWIHVWESVYTMLESFPKYLPNTYLTY
                     YLLSKETVEHSNINRTRANEVMESREKDLFEGVRHYLETGEISEKAFYAGSHGDWISD
                     LAVSIKNDTRSRFLVITENRGAIPNMPYDAMVEIPAYIGKDGPEVIARGPIPLFQQGL
                     MMQQLNSEKLIVEAYIENSYEKALQAFTLNKTIPSMNVAKEILDEMIEVNKEYWPELR
                     "
     gene            29113..29871
                     /locus_tag="KR250_000638"
     CDS             29113..29871
                     /locus_tag="KR250_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0066604.1"
                     /translation="MNIINKIFWQEDRIGMITNNLEADTHSHCMLQLFLGIEDSIEIT
                     VNEKLVKCNCIIVDKNISHSFSARKKVYYSAIIEPTSIYAEQLTSKMNDFGYWICDND
                     GLEKLRQQGTFLISNSSKEQYLRFMEMLNNYLNIPITLKHYDDRITELFNLLHTCNCD
                     NHTISSFADKVSLSASRLSHLFKEQIGIPLKSYILFHQMECAFRELLSGKNVTEASMT
                     AGFDTPSHFAGTVKRMMGMPVSLSLKDSEFLKVY"
     gene            29918..30637
                     /locus_tag="KR250_000639"
     CDS             29918..30637
                     /locus_tag="KR250_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HBH0066605.1"
                     /translation="MNKEYLEETKMLNYNEPQLKLLVSSKNWLELDDFHKIKSIYEFV
                     QNDILFGYNASDMLSATQVLNDGMGQCNTKATLLMALLRAVNIPCRLHAFDVTKDFQR
                     GATSKLISLLAPKYILHTWVEVFYQDRWIALEGVITDKKYLEAIQKKFFNHGGTFKKY
                     AIATNDLKNTSIDWDGKDTFIQKEAIVYDYGIFPSPDVFFSTHSQHMSKLKNFIYVHL
                     IRKIMTKNVCKARNNYIDKNE"
     gene            complement(30761..32161)
                     /locus_tag="KR250_000640"
     CDS             complement(30761..32161)
                     /locus_tag="KR250_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HBH0066606.1"
                     /translation="METQSNNKKGNLIIAIVMTGAFISSLSQTLLSTALPNIMSDFKI
                     TADVGQWLTTIYLLIAGIIVPTTAYLINRFSTRKLFITSMSIFSIGCIIALFSNNFST
                     MLIARVLQAMGSGSLMPLLQVIILYLCPEEKRGAAMSLVGITVGFAPAIGPTLSGWLV
                     DSFGWHSLFLFLSPIAILDVILSFILLRNVGETQKLKLDIPSIVLSSLGFGGLLIGFT
                     NQGNYGWTNIATYLPILIGIMSLILFTLRQLKSKEPFLELRVFKNKPFLISTILIMIV
                     YASMMSATLMIPLYVQSVRGFSALSSGSLMLPGAILMVVLNPIAGRHLDKYGPHALSI
                     LGTGCLFLGTLSFAFLGRDTSLIHVSLMYCIRMIGISMVLMPLTTWGIKTLDRELISH
                     ATAINNTLRQISGAIGSAILITIMTSATKKAHMSSNMLSNIHGIDVAFSIAATLAFTG
                     LIVSICFIKRHQIIRS"
     gene            complement(32165..33073)
                     /locus_tag="KR250_000641"
     CDS             complement(32165..33073)
                     /locus_tag="KR250_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBH0066607.1"
                     /translation="MKGDNKVTEKQRLIITIAQKIFDQKGFQNTSISDVVKECKMSKA
                     TFYKHFETKESFICEIINYYDEKFLEIIHSINENNDIPASEKLNRKIIAVWKNIFSRT
                     TINTYIRENFSEEQRKATSKLQTKSRANLLNEYKLSLFDNYGDKIEKIIFDLVFLLDA
                     LIHQFIYIIHVQKREINVYFIAKFTIQILDLVVENMDNLNPLIEKSMFLHKQEDEVYF
                     SLHNKSLFFKMIQDIEELIKTDTSLLENPKLLEAIKKLYVEGKNQQYDSLIMDAMIAY
                     LEKEDTLRPKVLLLNSIKNQLKKETL"
     gene            33334..33981
                     /locus_tag="KR250_000642"
     CDS             33334..33981
                     /locus_tag="KR250_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBH0066608.1"
                     /translation="MKKKICVAIIFALFIVFVEMFIDSSSEGSKIMTAELSKYRKGVN
                     VLSINNIIVANKKYSLPKDYSPQESSEARDAFYKMNKDAQKSGLNLKAFSTYRSYEYQ
                     DRLFKSYVKEHGEKEANRFSAKPGESEHQTGLAFDIGGDDQSCWANKKFNNTKEAKWL
                     YENAYKYGFILRYPEGKENITGYMYESWHYRYVGTEHSKNFAMNNLTLEEYLHIN"
     gene            34305..36944
                     /locus_tag="KR250_000643"
     CDS             34305..36944
                     /locus_tag="KR250_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBH0066609.1"
                     /translation="MSYKSTYQEVIKNLDSSLSGLSKERAEELLEKNGANELKEADKV
                     PTYKLFLESFKDPLVIILLIAALVQIFLGETVESIIIFAVIIINSVLGVVQTKKAESS
                     LESLKNLSAPNAKVIRDNKKMTIPAKNLVVGDIVFLEAGDYIPADGRLIEAQSLKVVE
                     GMLTGESEPVLKHTEKISEDVSLGDQKNMVFSGAVVVYGRGSFVVTGTGMDTEMGKIA
                     GLLESAENKLTPLQQKIEDFSKKLGVGIVILALLIFIIQVARNYFMAGNGEMTQIILD
                     SFMFSVAVAVAAIPEALSSIVTIVLAVGTNSMAKKQAIIRKLPAVETLGSTSVICTDK
                     TGTLTQNKMTVVDYYMYGTNNDELDKEKVNYSYHAKLLTMVSTLCNDSHITSDGKEVG
                     DPTEVALINYSSKNDLDYDKLRSKYIRINEIPFDSDRKLMSTVNSIYGDYIMFTKGAP
                     DIVFSRCKYALKNGSKVDITDEIIEEYKHKNEEFSNRALRVLAFAIKDVPEEDFIPSI
                     DDEHDMTLVGIMAMIDPPREEVMDAVKEAKSAGIKTVMITGDHKTTAAAIAKEIGIME
                     EGDLALTGKELDALSEEQLYEKLENISVYARVSPENKIRIVKAWQHKNNITAMTGDGV
                     NDAPALKQADIGIGMGSGTDVAKDASAMVLVDDNFASIVNAVEVGRTVYSNIIKSITY
                     LFAGNLGAIVSIVFAVFAGWSNPFTALQLLFINLVNDSLPAIALGFEKSEKNIMNKPP
                     RHPDESILAGGTLKAVAIRGCIIGVVTIIAQYIGLQVSPELGVAMAFSTLILARIMQT
                     LPARSNNQTVIKLGFFSNKYVIGAVLVCLGLYSLTLIPVLRGIFSIPTTFGLYQLGIC
                     LGLALISSILMELNKLINRSEVK"
     gene            complement(37002..38105)
                     /locus_tag="KR250_000644"
     CDS             complement(37002..38105)
                     /locus_tag="KR250_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate aminotransferase family
                     protein"
                     /protein_id="HBH0066610.1"
                     /translation="MKLKINKAIEMQLKKSYAIESGHSEDVFEIDCGEGINTVSYSNK
                     AVEAFNALKFDMIRGYPHSIALKDNIVDYWKDFIALDTDRICLADGSIHVIYLLNRLF
                     IEKGDKVLGYSPQFSEYETDIKMHGATYDYVLLKKEDNFKFNEKEFIEKINPEYKVIY
                     IDNPNNPTGQIIHLSSIENIVKEAAKYDIAVMVDEAYGEYMPKENSAVKLLNNYDNVI
                     ALKTFSKGFGLAGLRAGYAVLPEQLVSPIKKISTPYEVSEISRSIAANLLDDVQFIEE
                     LKEKTKDIKNQLLIPWKNLNIAETSDTVSIMTVEHKNKDIDLQQEFAKLKIRVISGSD
                     FTGLDKNFIRFRMPEEKELPEVIKAFQIIDNIE"
     gene            38466..39287
                     /locus_tag="KR250_000645"
     CDS             38466..39287
                     /locus_tag="KR250_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBH0066611.1"
                     /translation="MGYKLIVTDMDGTLLGNNHKVTDENKTALQKVIKSGINVTLATG
                     RAFDSAKCNVDFLKEDMPIIACNGSLIREQNGNIIYSNKIDTRTCLNILDVLDKYDIY
                     YQCNSIDSMLSKKIEGREDRLSVFLGSETEVIVKDDLREEIFKKDILKFVIIEEKNPS
                     ILDEIRKELRKVQGIKITSSWPNNIEVMNEGVDKGNAVKILAEKMNIDREDIIAFGDN
                     YNDIEMIKFAGLGVAMGNAEELIKQEADYVTDTNQDSGVAKAIYKFLELKESLSS"
     misc_binding    39366..39577
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            39664..40980
                     /gene="argH"
                     /locus_tag="KR250_000646"
     CDS             39664..40980
                     /gene="argH"
                     /locus_tag="KR250_000646"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HBH0066612.1"
                     /translation="MKLWGGRFRKAENQLMEEFNKSFGYDCVLYKKDIEGSVAHVHMQ
                     VKCGLLTEEEGKSITEGLKGILEDVENGKLVLDGEYEDIHSFTEINLIQRIGDVGKKL
                     HTARSRNDQVAVDMRLYAKEKANDLVGLISEFKATIKDVADKNPVMMPGYTHLQRAQV
                     VTFKHHLMAYYSMFDRDEKRIKNAIEILDESPLGCGALAGTTHDIDRSITCEQLGFKK
                     VVDNFMDGVSDRDYLLELMSDFSIIMMHLSRLSEELILWSSQEFGFVEIDDLYTTGSS
                     IMPQKKNPDGAELIRGKTGRVYGNLFGLFTVMKGIPLAYNKDMQEDKEGFFDSVHTLE
                     MCIQIMDRMIATLKVNEDKMKQAVKNGFLNATEVADYLVKNNVAFRDAHGIVGSIVIY
                     CEDNKKAIEDLTLEELHKFSDAFKEDIYDFIDYESILNKGIKKNLK"
     gene            41229..41567
                     /locus_tag="KR250_000647"
     CDS             41229..41567
                     /locus_tag="KR250_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2325 domain-containing protein"
                     /protein_id="HBH0066613.1"
                     /translation="MNLLVIGGHERMEKDYMVMAKKKGYKTKVYTTMSSKLNNSIGRP
                     DAVVILTSTVSHKMSRTVESQAKKQDILIVRHKNSSKVAFNECLDMVDECLGNCSKCK
                     LKDKQKQKNI"
     gene            complement(41694..42983)
                     /locus_tag="KR250_000648"
     CDS             complement(41694..42983)
                     /locus_tag="KR250_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBH0066614.1"
                     /translation="MKRNLSLLLICLLIFTSFLGSSNISFADNEPAIVAKHAVLMDYE
                     SGKILYNKDGNSKLYPASTTKVWTACLVLKEVKDLNQVIEIKDLPQIDGSSMYLKNGE
                     SFTVKQLLDALLVHSANDAAFVLARYVGGGNVQKFIDLMNSEAKKIGATNTHFNNPHG
                     LPDPNHYTTAHDMALIAREAMNNDTFRQIVKTKSLKFEATKAYPYERYFVNTNKFLTS
                     HDKITYKGQPINIKYDIVDGIKTGYTDAAGKCLLSSAVKDGRRVIVAVFNSTNADLYL
                     DSRILIDYGFDNFKCATIVDKEKYTDTKKVLFTKQHELIYEPKNSYRIFLEKDESQGN
                     YDTKTELNKIDLPIKKGAKVGTLNVYNNGKLENSIDLIAKNNLDSSLPFFTGNNILMT
                     FVKIIAGILVLLVLFIIASNIKKKKRKKARSKKNMKK"
     gene            43063..43767
                     /locus_tag="KR250_000649"
     CDS             43063..43767
                     /locus_tag="KR250_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HBH0066615.1"
                     /translation="MKKKVKFISFFVLFILIISSIIVYKSLIFKDDIYVVSKKNLPNE
                     KTHVVENEIKGEDNNHNKVTKDIDKNFQNNKITIYISGAVNRPGIVTIESDKRLYDAV
                     ELLGGTTKEADLNGVNLSVRLEDEQHYIIPKIGEATSVTSNDDSNKPNQRNESRGEPK
                     NKSNKNISNESKVNINVATIEELDSLPGVGEATANKILQHREENGQFSSIEEIKNVNG
                     IGDKKYENIKDLICVD"
     gene            43859..44905
                     /gene="selD"
                     /locus_tag="KR250_000650"
     CDS             43859..44905
                     /gene="selD"
                     /locus_tag="KR250_000650"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:43907..43909,aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HBH0066616.1"
                     /translation="MKKEQKKLTEMTTAGGUAAKIGPEVLASVLSQLPKNDNIENLLV
                     GLDTADDAAVYKLNDDMALIQTLDFFTPMVDDPYVFGQIAASNSLSDVYAMGGKPLVA
                     MNIVCFPSCHDMDVLAEILKGGFDKVKESGALLVGGHTVDDKEPKYGLSVSGIVSPNK
                     VLSNATAKPGDKLIITKPIGVGVLNTAMKEGMVEQHIADKVIEIMIHLNKYAAMSFEK
                     FDVNSVTDITGFGLLGHTLEMAKASEVSIEIESKHVPIIEGAIEMAQMGIIPAGMYKN
                     MHYVSKDVEVVGNIEVAVQDILYDPQTSGGLLISVKEELAEELVKDMKLNGAIEAKII
                     GSVVPKGEKYITVL"
     gene            44923..46335
                     /locus_tag="KR250_000651"
     CDS             44923..46335
                     /locus_tag="KR250_000651"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HBH0066617.1"
                     /translation="MIVLSKRELFAMLPSVDEVLSDNRIVEIINEYPRSLVLESVREV
                     IDLKRQFILRLKEDASNSVTIEFGEIIESAIERVKLNYSLSLKKVINATGTVIHTNLG
                     RSLLSEDIKDELWCAASRYSNLEYDLDNGERGSRYSHLTSTIKRLTGAEDVLVVNNNA
                     AAVLLVLSTMAKGGEAIVSRGELVEVGGSFRIPSIMALSGAELVEVGSTNKTHLKDYK
                     EAITEDTNVLMKVHTSNYRIMGFTESVSIEELVNLGKKYKLPVIEDLGSGVFIDLSKY
                     GLSYEPTVLDSIRQGADVVTFSGDKMLGGPQAGIIVGKKEYIEKMKKNQLTRALRVDK
                     LTICALEATLRMYLDETKAIENIPTLKMLTYKIEELEVKANKLFEKITALNLNANINI
                     EDGFSQVGGGSMPLETISTKVISITPEHMNVSSLEKKLRLSEAHIIARVYDNKYVLDV
                     RTIFDDEFDVIVEELRKAFN"
     gene            46449..48332
                     /gene="selB"
                     /locus_tag="KR250_000652"
     CDS             46449..48332
                     /gene="selB"
                     /locus_tag="KR250_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HBH0066618.1"
                     /translation="MKNVVIGTAGHIDHGKTTLIKALTGRETDTLDEEKKRGISINLG
                     FTYFDLPNNRRAGIVDVPGHEKFIKNMLAGASGIDIVLLVVAADEGVMPQTVEHLDIL
                     SFLNIKNGIVVLTKSDTVDDEFRELVKEDICEKIKGTFLEDAEIIEVDSISKRGIDTL
                     IEKIDKMSDDIEDKNENSPARLNIDRVFSIKGFGTVITGTLIEGKISIEDDLVIYPQV
                     LKTKIRSIQVHGENKDTAYAGQRTAINISNVKVDDIKRGDVLAAPNSLEESMMLDVKL
                     SLVNHLDKGLKHWDRLRLYHGTREILCRAVPLDKELIESGESGYVQLRLEESIVSKKG
                     DTFVVRRYSPMETIGGGVIIDPSPKKHKKFDEKVIEALKIKEKGELKDIIEEYLKRNL
                     KNYPNIKEIMSYSGAHEEDVKRALETLISEDKVFIIGNMYMHINQYNKLKENTIKLLS
                     EYHKKYRLRKGILKEEVRSKIESNFKTREMDILLEKLSTENAIKIENNIVSLLDFEVI
                     LNDKQKEIAKKIEKRLKSCGVSSILTIDEVSEGNHNYAEVLESMIGNKVEKLDDLYIM
                     DKDIYENAKNILINYIKENKEITLGEYRDLIDSSRKNCMIILENFDRNKITKRVENKR
                     ILF"
     gene            48437..51175
                     /locus_tag="KR250_000653"
     CDS             48437..51175
                     /locus_tag="KR250_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation-associated two-component system
                     sensor histidine kinase"
                     /protein_id="HBH0066619.1"
                     /translation="MKNIIPDVKPDIKEREDMSLLFIIMGSVLLLFIIKDIEKYETLL
                     HAIYTIFLVATGVIIFNTTKFKVDSFSTFLGLIFIATGVLECIYLFNSLGIKTKSIME
                     INITISAITDLFPILGVYLSFKFVKDNKQIYSSVILFIATATLTITSLFIISGICDYL
                     GNGKSQYALGVIISIFIIIISIISGIELNSSSNKGKWEYDEKKIFNRIIVIIILSRVP
                     NLLHIVIDNRNVERILSQIIINVALYYLYNYIVSKNIKKTALELHDTNEELTKKTESL
                     KEKNKKLIYETEKIEELKGILMKRESRLQSTLDVAVNSIVVFSKDGEITYANKSFRNT
                     FGDYKEQDKLTDKIKNFNDLIESIHYVFVHEKNAEKLISTSDNKVYQAIFAPLIIASQ
                     TEGVLCVLEDKTKKKEYERKLIEANKRSKDFLESIGDGIVVLEGNNKIYVNNACREIF
                     KNNLESIDFSLVCKSEESIEKRYVIDGEVKYVEMSFSQYTNAGTNKTIIVIRDTTSRK
                     ISQIKLEESQSSYSRFIDILPDGICLLKEDLSINYANKSFLDMLAFTNIDDIKDSNIK
                     LLMNANTEEKMKFTDNMAKVLNENRSILLLEYELINSYDDIVEVELSALPFAIYNTRY
                     IMLIIKDLVHKKYSEQAEKELLERFKTDKIKTEFFANMSHELKTPLNVISSSNQLVDS
                     FYRNEKIKDYNNNIKSHVDLVRQSSYRLQRLINNIIDLTKMESGFYTLKLAKYNIVSV
                     IEDLFMNIEEYALRKDIKILFDTDLEEINVYIDKVEIERIMLNLLSNCIKFTDNGGWI
                     YVSIHYKIDKVIISVKDTGVGIPQDKLELIFEEFSQVDKTLSRNTEGSGIGLAIVKNL
                     VSLHGGDIEVVSEVNKGTEFLISLPMKGFSSEHYTEDKRIYNIQEKIKIEFSDIYY"
     gene            complement(51294..52238)
                     /gene="manA"
                     /locus_tag="KR250_000654"
     CDS             complement(51294..52238)
                     /gene="manA"
                     /locus_tag="KR250_000654"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HBH0066620.1"
                     /translation="MEPLFLKPIFMDRIWGGTALKDKFNYEIDSPTTGECWAISSHKN
                     GDCLIENGKYKGKKLSELWNKNRELFGNTPGDKFPLLTKILDANDNLSVQVHPNDEYA
                     KKNENGELGKTECWYVIDCSDDAEIIIGHNAKSHKEFVNMVNNNEWDKLLRKVNIKKG
                     DFFYVPSGTIHAICKGTLILETQQNSDTTYRVYDYDRTDNSGNKRELHVQKSIDVTNV
                     PHINFDTDYKIVSTSDFKCTTFVSNEFFSVYKLDVFGKCNFTHNTPFSLYSVLDGNGK
                     LIHNSVEYNLKKGMHFILPNDFGDFSFEGNLEIICSHI"
     gene            52411..53772
                     /locus_tag="KR250_000655"
     CDS             52411..53772
                     /locus_tag="KR250_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBH0066621.1"
                     /translation="MELFKAESKKYKKEFLKLLEQWVSIPSFYDRKTVSKDMPFGKGV
                     YDALNWFENLGRENNFKVKNIDNHAVQIEYGNGKEYVDIFGHCDVVNPGEGWDSEPFK
                     LNIIGDKLVARGVSDNKGPMIVNFLALKMIKDLDINLKRKVRLIAGGNEESGFKCIKH
                     YYSKEPYGVCGFTPDAKFPVLNGEKGGAIIKLISNIDDKSLYISGGIEFNTIPDKVYI
                     KNVEKLGKDNICFDINNISINYDNGNYIVYGKGGHSSKPEKSINPILATIKLLSENID
                     EKWTKDLYKLINQDNINGNLFGLNIEGKCGILSMVPTIINIVDGKLEVVLSVRYPEIL
                     TIEDIIKKFNLYMEQNNINKFELIGENLKQANYIDRNSKLVRSLHDIYIKYSGDLKND
                     VRVTSAGSYASEMNNSVIFGCEFPDGSFGNVHSANEFASLDRFITAIGIYAEAIITLC
                     NKI"
     gene            53860..55749
                     /locus_tag="KR250_000656"
     CDS             53860..55749
                     /locus_tag="KR250_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBH0066622.1"
                     /translation="MITKKHAIIVKSLSNKDGYMTSNELAIKLDVSTKTIKRYIADLN
                     SVLSKYDLVIASSRGIGYKLSGSKNNIARAVKEANKYIDGFLDDSEESRMSNIICMLI
                     NRNYMSIEAMAEELNLSIAAINKLSSKLKRKLEKYDLVIKSKPYYGSYIHGEEINIRQ
                     LITDYAIKLDEKSKVKVFLDDISENDIHCIESILEKHLREKDTIISDKDFNLLLSKII
                     VSVFRSKRGHSNNINLMDTSYRFHNYYFIENLMKEISDKIGFKLIEDEVIYISNYSGV
                     IAYKGTQGRKNTSEIEERISTVISSALQDIFLISGSDYTKDDEFMVAISDHIKRFLNR
                     ARANVKSNNPLLHQIKEKFPIAFNLAVFLSNKLETEFNLKLDEDELGYIAIHFAASNE
                     RMKKNTSKKICIVCHYGIGTGQLISEKLKQNISDLSVVGVYPVRYLDMAISQDVDLIV
                     STVELKGYEKPVLYIENIFDDSLIENVNKAFYEKEERRKIISNMFDEKAFFSIKASTK
                     EEVIISLSNKLKERDFIEESSIKSIIDRENISSTEIGNLVAIPHTIVKGDKKSIIGVG
                     ILENPIIWDKQEVQLVFMVFFNTKEKHNFSIFKYLYNFIKDEGGVRGIIKICDFNKLM
                     ALIGN"
     gene            55764..56219
                     /locus_tag="KR250_000657"
     CDS             55764..56219
                     /locus_tag="KR250_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBH0066623.1"
                     /translation="MGTNIFNKEYVFLNVDAKSKVEVLKFISKKAKDLNLAEDESLVY
                     EGLMAREEQFTTNLGESIAIPHTKNDAIENPAVVVLKFNEDVVWNEGEDKVKLAISLL
                     MPGKSKENIHLKLLSSLSRKLINKEFKDSLLKSDNVEEISNLINEALGL"
     gene            56278..56595
                     /locus_tag="KR250_000658"
     CDS             56278..56595
                     /locus_tag="KR250_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HBH0066624.1"
                     /translation="MNKKLVALCACPMGLAHTFMAAEAIEQAAKALGYEAKVETQGAD
                     GVQNELTRDDILGATMIIHAVAITPEGMERFDGCEVYEVELQEAIKNAEGVIKEIEED
                     LGI"
     gene            56620..57882
                     /locus_tag="KR250_000659"
     CDS             56620..57882
                     /locus_tag="KR250_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HBH0066625.1"
                     /translation="MAIKKKVINSSGASTTGGNNVGKSTPSSPTQSKGNGKWKEISKH
                     IMTGISYMIPVLVMGGLIGALSQLIPYAILGLDPSVGIVDAMNSGEFTGFKLSLLNIA
                     QLMSNFGFTLFGFAIPLFAAFCANSIGGKTALIAGFIGGYIANKPVGVPQFVDGQWTE
                     VVPVASGFLGAILIAFIIGYFVKWLNKSIKVSHNWLAFKTTFLIPLIASLACMVLMIF
                     IITPFGGLINESMKNFLTAAGAAGEYVYATALAAATAFDLGGPINKAAGFVALGLTTE
                     NVLPITARTIAIVVPPIGLGLTTLLDKRLVGRRVYDRQFYQAGKTSIFLSFMGISEGA
                     IPFALERPGFVIPLNIVGSVIGAITGIILGAIQWFPESAIWAWPLVDNLFGYIIGIAV
                     GAIFIAVGNIFYRNKLIKDGKLVVDYID"
     gene            57987..59051
                     /locus_tag="KR250_000660"
     CDS             57987..59051
                     /locus_tag="KR250_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBH0066626.1"
                     /translation="MDKIEKVREYFREYDIDGFLINSPTNKFYVGNLFSSSGYVFITK
                     ESQYIIVDFRYFEEIKRKSSLFNVVLMDKTRTHFDIINDICREQNIKEIGFEGNEVSF
                     DLYRSMSNKLSATLKSVDLSTLRETKNEDEIKYIKKACEIVDATFYHIVDFIKVGMTE
                     KQVENEIVRIIKDLGGQKESFDTIVASGLRGALPHGKASEKVIEYGDFVTFDFGAKYN
                     NYCSDITRTICMGTINKELEEIYNIVRKANEECIRVLRPGMTTGEIDKVARDIIGSYG
                     YANNFGHNLGHGVGIMVHEYPALAPESNEVLKEGMIVTIEPGIYVPSLGGVRIEDDVL
                     ITQDGCMRLTTSTKDLIVVS"
     gene            59105..60283
                     /locus_tag="KR250_000661"
     CDS             59105..60283
                     /locus_tag="KR250_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HBH0066627.1"
                     /translation="MYNFDDKPDRVSEKCRKWDLNIIRDKFGDIREDFIPMWIADMDF
                     KIPTEIENKFIEAVRRGVFGYTYCYDEFYDSVIGWQKNMHEVNVEKEWITLTYGTVST
                     LHYVVQAFCKKGDSIILNTPVYDPFESSAKKQGVNVICNTLDVVNDRYYINFDKLEVQ
                     IKENKPKLMMFCTPHNPSGRIWTIEEMTRVATICKENNVILVADEVHAEHIHYGTFNS
                     ILKIGKELLENVILLTSPNKGFNLGGLKTSYSIVINKDIRDKFRNKLKQNSITSPNVF
                     GIIGLITAYNECHEWLSGVNEYIKSNYELLETWVNKYNKIKLMKMESSYLAWMDISGL
                     GISASEFTDKLAIDTGVLLEDGSHFVKDGEKYVRINLGTQKENVIEALNRMDLFLKSL
                     "
     gene            complement(60474..61532)
                     /locus_tag="KR250_000662"
     CDS             complement(60474..61532)
                     /locus_tag="KR250_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HBH0066628.1"
                     /translation="MNKKKITFIFVILILALILFSFFRSSSQNTKYSKTSYYLGTVNE
                     VTVFNVKESKSDKILNECDSILRYIDNKMSTHIPGSDVSKINDNAGKKFVRVSDDTFF
                     VVKEAIQYSKLSDGYFDITIGPLSNLWAIGTDKAKVPSDSKIQKLLPLIDYKNIILDE
                     KNKSIKLTKENMKIDLGAIAKGYAADKIVAYLKSEDVKKGLVNLGGNIFTLENGKNDK
                     PFKIGIQDPTSKNGESIGNIETTNKSVVTSGIYERFIEKDGKIYHHMLNPFTGYPFEN
                     NLSSVTIISDKSINCDALSTSTFGLGLEKGMNLINKIDNVDAIFITKDKKVYLTKGIK
                     DNFKLTDKSFSIEKLNKQ"
     gene            61599..61970
                     /locus_tag="KR250_000663"
     CDS             61599..61970
                     /locus_tag="KR250_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HBH0066629.1"
                     /translation="MKKKDILLILGILVVITACYGIINVINSKNAGNIEIYVDNKLYK
                     TVSINAKEEFKIESRGGYNIVKIHDKGVEIVDASCPDKVCVHTGFINKPSQSIVCIPN
                     RVSIKINTNEKNDNQEDIISK"
     gene            62007..62681
                     /locus_tag="KR250_000664"
     CDS             62007..62681
                     /locus_tag="KR250_000664"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HBH0066630.1"
                     /translation="MVNLGNDWDELLKEEFEKDYYLNLRKFLIEEYKTRQIFPNMHNI
                     YEALKHTSYKDTKVLILGQDPYHGDNQAHGLAFSVQPQVKTPPSLLNMYKELKDDLGC
                     FIPNNGYLMPWADQGVLLLNTALTVRAHEANSHKNKGWEIFTDRVISILSEREDPVIF
                     VLWGSNARKKVELIDTSKHYILEAPHPSPLSASKGFFGCKHFSKINEILKKLGKEPIN
                     WQIENI"
     gene            62930..63769
                     /locus_tag="KR250_000665"
     CDS             62930..63769
                     /locus_tag="KR250_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HBH0066631.1"
                     /translation="MNFKKLSNAKVSVMAVVLSVGILSGYSALNKQVTLVVRGEERTI
                     STFSSNVGDLLESQNISYDSNDIVTLDENTKLSNGDKIEVIDVKEKTVVETKDIPFEV
                     TVVEDGSLLKGDSKVKEEGKSGKSELIYKETYHNGKKVEKEFVKKVVKAEPVNKVVSK
                     GTKVEMQVASSRGESSRRMASSSSSSSSSNNGKNMRVVATAYTGHSITATGTKPKWGT
                     IAVDPKVIPYGTKVYIPQFDMVFTAEDCGGAIKGNKIDIYMNDSKTVYNWGRKTIDIR
                     ILK"
     gene            64013..64109
                     /locus_tag="KR250_000666"
     tRNA            64013..64109
                     /locus_tag="KR250_000666"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:64047..64049,aa:Sec,seq:tca)
     gene            64264..64971
                     /locus_tag="KR250_000667"
     CDS             64264..64971
                     /locus_tag="KR250_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HBH0066632.1"
                     /translation="MENNFTTRTSFLVGDDGIKKLNNSNIIVFGVGGVGSFTVEALAR
                     AGVGNITIVDFDDVDITNINRQIPALHSTVGRYKVDVMEERILDINPNINIKKIRSLY
                     NKDTSDEILTERYDYVVDAIDMVSSKIHLIETCEKKGLKIISSMGMGNKLDPTKIVVT
                     DIHKTSTCPLAKVMRKELRDRGIKKLKVVYSTEQPIELKKKVMNGRKVTPGSVSFVPS
                     VGGLIIASVIVNELLGQ"
     gene            complement(65113..65949)
                     /locus_tag="KR250_000668"
     CDS             complement(65113..65949)
                     /locus_tag="KR250_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBH0066633.1"
                     /translation="MRNKIFLSILIIISLLIGCDKKSLLSIHMIDVGQGDSILVQTPT
                     NKNILIDGGDEDSENIIISYLRQKRIKTIDIIIATHPDSDHIGSLDNIIKKFNVNSIY
                     MPEQSTDSKAYQNLINSCTDKNLSIQHLYKNDVLNIDNNINIYVLSPSYIQEESNLNS
                     IVFKLTFNDNSFLFMGDAEEENEKEILHSFKLNNINFIKIGHHGSNSSSSLEFIKKIS
                     PDIAAISCGYKNQYGHPHREVINNLKQNHVSIYRTDRIGDIVFYSDGEIIFTKYNYEI
                     DT"
     gene            66137..67042
                     /locus_tag="KR250_000669"
     CDS             66137..67042
                     /locus_tag="KR250_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2156 domain-containing protein"
                     /protein_id="HBH0066634.1"
                     /translation="MFFKDIELNSKKELDPYFDLVDYEACEYCFSTLYMWQHVYKTGY
                     YIGEDFAVLVGEYEGDSFSILPLAKKDKLPEVVDFVLEYFSKNNKKIYLRGITTEVVE
                     FLKEKYPGRFEYIEERDLFDYIYDAESLRTLAGKKNQKKRNHINYFLKEYAGRYEAKL
                     LDKENFDECLVLMKEWESNKEENNEFDESMDDELIGIKKIFNHYDILKDKVKVFGVYV
                     DGKLEAFSIGELLNPNMALIHIEKANPDIRGLYPFINQQFLVSEFKDVEFVNREEDLG
                     IEGLRKAKLSYHPCRFVEKYSVREA"
     gene            67051..68235
                     /locus_tag="KR250_000670"
     CDS             67051..68235
                     /locus_tag="KR250_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBH0066635.1"
                     /translation="MEIRYAKEEEIESIKDIWSYCFNDTESFMKYYFNDKYKSENTVV
                     ALDEGKIISSLQLNQYKLLLNSKVYNTSYVVGVSTLPEGRGAGYMNKVMKFTLNELYK
                     KGQLVSILMPIDYRLYRRFGYEHCYDQIEYTINTDDLKNFKSSGKMIKSNLSQIDDLI
                     QIDRTFLNEVNGNVLKDEHYYENLFKEIQSEDGFLYIHEGNEKDGYIVYFLQEDKMFV
                     RELFYKNIDALKSMLKFIYNHNTQCKIVTISTPTIDKIRFILDNPKDSDIKIKPFMMG
                     RVINVKKFIEDIDIEKDINSSFNLLIEDKFIDENNGLFKISIQNKKVSVEQLDKKGAE
                     KPQEDFDIKLDINTLTQLSFSYIDVNEAIFLNDIKDVSEETLETLNCIFSKKNNYINE
                     YI"
     gene            68350..69651
                     /locus_tag="KR250_000671"
     CDS             68350..69651
                     /locus_tag="KR250_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HBH0066636.1"
                     /translation="MGVIIIQIRRPLLIILMFVVAISFIITNNTKNDEELNDKIITIQ
                     GVVKGKIEKKRYNQYKVGVFLINDYTKNKNLKIGQKVNITGKFKSLDKMKYDDFDYGR
                     YIKSTGYKGIVYINNYKIIDKNKLYSLIGEIKFYISKTYRYLYKKNSDFINSILLAEV
                     ENLTEEQKEIFTRTGTSHVISISGLHTGILCVIISFLLRGINKLYKLLILSIFITLYC
                     IMVGASPSIIRSIAFVMIFYLSIFIDRKKDGISALSLIGIILIMNNPYVIYNVSFQLS
                     FLATLSILIFYNKINSIIKLSMVSLTISSNILTLPIIYYTFKGIPLLSIIGNLIIVPF
                     VGVIMYLSIASLIVFKVSVVIAKIISFFNSTFIESIFFLLEKISNLSFAYINIENPKF
                     YIVVIYYIGVFFYIFYIEGKEIKEQENELQGYYKECKREKL"
     gene            69605..70648
                     /gene="holA"
                     /locus_tag="KR250_000672"
     CDS             69605..70648
                     /gene="holA"
                     /locus_tag="KR250_000672"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HBH0066637.1"
                     /translation="MNYKDIIRNVKEKNFEKMYLFYGREYYLIENAIKAFKDSLNEGM
                     LDFNLDIIDGKEIVLNQLISSIETLPFMDDRKIVIIKDFELLKGKKKNFTDSDEKYLI
                     EHLDNIPDTTTIVFVVYGDVDKRKSLVKKIGNNGIVFDCDKLSDMDLFKWIKKSFALN
                     DVIIDNSQIMYFIEQEGYRDKSSEKTLSDLENEINKISSFVGKGNNVTNDVIDKLSQK
                     KVENDIFKLIDYIGEQNASNAMKILNDMIQEGESVLGIFSMIARQFKIIMQVRQLQLD
                     GYSTKLIADKLKMHQFVVGKALKQTKNFSDDIIVEILNYILESDYKIKTGLIRDTLAV
                     EMLVSRYCKREAI"
     gene            complement(70835..71101)
                     /gene="rpsT"
                     /locus_tag="KR250_000673"
     CDS             complement(70835..71101)
                     /gene="rpsT"
                     /locus_tag="KR250_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HBH0066638.1"
                     /translation="MANIKSAKKRISVIEKKTALNRVRKSQIKTAIRRFEDAVAAGNR
                     EDAVAKFQYAQKRIYQVASKGTIHKNAAARKVAKLAQKLNGMNA"
     gene            71697..72218
                     /locus_tag="KR250_000674"
     CDS             71697..72218
                     /locus_tag="KR250_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH0066639.1"
                     /translation="MDIAKLILNPARLRILQYIRLYGSVRTSDIVKYLNDIPRATVYH
                     HVKILEENNMIEVIKENRVRGTIEKVYALKEYTTSMEGETFVALSTAFYVGLMQEMNE
                     YFSRKEQDHKKDNVFFSSALLYMSDNEYENLLKSIADLLKPYIEQKPKSNLKLRKLSI
                     ISSPPVADNDKTK"
     gene            72252..73028
                     /locus_tag="KR250_000675"
     CDS             72252..73028
                     /locus_tag="KR250_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBH0066640.1"
                     /translation="MKFHEFGNKNRPHIMLIHGGGNAWWNYLRQAEVLSNKYHVILPT
                     LDGHGEEYETTYISTEDTADKLISYIDENCSGHLFALCGVSLGGQIVMELLSRKPNIT
                     KKAIIDGSICYPRPLMARFCIAGIRFFGGLLFSEKACRFQIAMMPKLLPENMQYPDEI
                     KAYYMTDMPHVRKETFYNMYRTYMMNYTLKENVKKTTAQVMYWYGEKEMKCVKKSAKL
                     FQSYVPSCEIYEAKSYNHGYLSLYLPNEWLEIAEPFFQKE"
     gene            73370..74323
                     /locus_tag="KR250_000676"
     CDS             73370..74323
                     /locus_tag="KR250_000676"
                     /EC_number="3.4.24.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPR endopeptidase"
                     /protein_id="HBH0066641.1"
                     /translation="MISVRTDLALEASEMCEKSQEGSSIPGVKIETKELENCIVTKVE
                     VIDRQGAEIMNKDIGKYITLESNLMKFDDDESREEMISYLKEELVDIFGQDKNKKTLV
                     IGLGNRNITSDALGPKSVSKTLVTRHLFKNYNKDYDDDFTEVSALSPGVMGVTGIETS
                     EIVKSLVEKVKPDRVVAIDALASRKMERVNSTIQISTAGISPGGGVGNTRKSLTKETL
                     GVDVIAIGVPTVVDAATLTIDVLDMAIDNLIAQSEETESFYEMLKKLKEEEKYHLIKD
                     SLDPYDKNLIVTPKDIDDTIENLSIIISEGLNRSLHPGRLV"
     gene            74341..75360
                     /locus_tag="KR250_000677"
     CDS             74341..75360
                     /locus_tag="KR250_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein P"
                     /protein_id="HBH0066642.1"
                     /translation="MFKKCIKVVTLTFILVCILPGKSLALNQDDFLKFLVNSSYPEAK
                     VEGNDTENKKNNKNKETSKENKEESKEGNTKSKDASKVDNKKESEKEYIKLYVGKENV
                     PDIESKNSDTTETNTTSSSDYKDDLRVTKENPRILIYHTHGCETYSNSPDGNYHSRDK
                     KNSVMEVGSALTSALDSKGWGVVHTTKYHDYPSYNNSYASSLKTIQSILPKYNSVDIA
                     IDLHRDARDLTNPATKEKDHLKYTTMINGERVSKFFFVVGGKNTNRKQLRALAEDITA
                     FAEKKYPGLVSPIVEKDYARFNQFAVKNHMLVEIGNNATSVEESKATTKYLAEILDEY
                     FKQKN"
     gene            75391..75642
                     /locus_tag="KR250_000678"
     CDS             75391..75642
                     /locus_tag="KR250_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0066643.1"
                     /translation="MSRLEKNIESKSKLRKYKYIMRILFLITMIICTSVCVFIVDSNA
                     KSMLGMKQYDFPSIKFDITKFDFDIPNFDIHEIIKKFNK"
     gene            75924..77729
                     /gene="lepA"
                     /locus_tag="KR250_000679"
     CDS             75924..77729
                     /gene="lepA"
                     /locus_tag="KR250_000679"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HBH0066644.1"
                     /translation="MDNKQSRTRNFSIIAHIDHGKSTLADRLIQQTGLVSERDMKSQL
                     LDNMDLERERGITIKLQNIRLMYKAKDGNEYYLNLIDTPGHVDFNYEVSRSLAACEGA
                     LLVVDAAQGVEAQTLANVYLAIDQDLEILPIINKIDLPSARPEEVKNEIEDLIGLDSS
                     EAPLISAKTGLNIEDVLEDIVKNVPPPKGDNEAPLKALIFDSYYDAYKGVVAYVRVFE
                     GTVKKGMTIKMMNTNKKFEVTEVGVMAPGQTELSELSAGDVGYIAASIKDIRSCRVGD
                     TITDSNNPTEEPLPGYKKATPMVYCGIYPGEGEKYENVRDALEKLQVNDAALEYEAET
                     SAALGFGFRCGFLGLLHMEIMQERLEREFNLDIITTAPSVIYRVTKMDGEVVMIQNPA
                     NLPEPSEIKMIEEPIVKGDIIVPKDYVGVVMELCQERRGNMLNMEYIDDRRVMLHYDL
                     PLNEVVYDFFDALKSRTRGYGSLDYEVKGYVASTLVKLDILINKEQVDALSFIVHETR
                     AFPRGKAMCEKLKGEIPRHQFAIPIQAAVGNKVIARETISALRKDVLAKCYGGDISRK
                     KKLLEKQKEGKKRMRQIGSVEVPQKAFMSVLKLDE"
     gene            77878..79215
                     /locus_tag="KR250_000680"
     CDS             77878..79215
                     /locus_tag="KR250_000680"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HBH0066645.1"
                     /translation="MINIEIPKKVDYIIKELEKNGYEAYIVGGCVRDCLLERVPNDWD
                     ITTSARPEVVVELFEKTIPTGIQHGTVTVMIEHEPFEVTTYRIDGNYSDGRHPDSIEF
                     TNNIVKDLSRRDFTINSIAYNSKTGLVDPFNGYEDIQNKYIRCVGNPVDRFEEDALRM
                     LRAVRFSAQLNFKIAEGTKQSIHKKADLIKNVSIERIQTEFNKILVSDSSKLNLLKST
                     GLLKFIIPEICELEDVTQHNPYHIYDVQKHTLIATEVIEDELYLKLTMLFHDLGKKVT
                     KTTDKNGVDHFYTHSRESVKIAKKILKRLKYDNYTINKVLILIQYHDYRIEPKRKIIK
                     KLLNKLEDVELFEDLIKVNWADTLAKNPKYAKQKILNLIECEKEFKHIINQKECFNLK
                     DLAINGKDLISIGIKPGKDIGYILNKMLEIVINNPELNEKEILKEKALNIYTF"
     gene            79448..80761
                     /locus_tag="KR250_000681"
     CDS             79448..80761
                     /locus_tag="KR250_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBH0066646.1"
                     /translation="MHTQLQKTIGLSAALSTVVGMVIGSGVFFKPQAIYTTTNGAPGL
                     GIIAWLLGGFITITAGLTATEISAAIPKTGGMMIYIEEIYGEKLGFLTGWMQTVLFFP
                     GTSAALGVIFAQQASELLGMSPNNMANVLPIAIGVILFLALLNIIGSSLGGKVQTVAT
                     IGKMIPLILIIVFGFIKGQSSEVLNPFVGDGVNASNALGQVLIATLFAYDGWINVGAI
                     SGEMKSPEKDLPRAIVGGLSLVMAVYIIINIAYLWVVPASELATVTSPATLVATRLFG
                     NIGGKVITVGILISVFGTLNGYLLTGSRIPYTLAEMGTLPASKTLLKVNSGGSPVNSI
                     LLITVLACVYALSGQFNLLTDLTIFSIWVFYVLTFIGVMRLRRQKPDLHRPYKVPLYP
                     IIPIIAILGGLFVIINQILTSTVISLGGIFITLLGLPVYYYMKKR"
     gene            80933..82108
                     /locus_tag="KR250_000682"
     CDS             80933..82108
                     /locus_tag="KR250_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HBH0066647.1"
                     /translation="MLGLYVHIPFCVKKCKYCDFNSYKMDIDSKKRYIEDLKIEMELY
                     SNKLYKDNKYKNKECCSLNKNDKITSIFVGGGTPSILTSDEIREVFISIKEMFDIDEN
                     AEITIECNPGTLTLEKLKTMKEIGINRLSIGLQAIQEKHLNFIGRIHTYEEFEKNYKD
                     ALSVGFKNINIDLMYSLPNQTLCDWKETLEKVVHLNPTHISAYSLILEEGTELYNMYE
                     SNKFELIDENVDIEMYEYTINYLKSKGYNQYEISNYSKEGYNCEHNILYWECEHYIGI
                     GAGASGYINENRYNNVESLEDYHLSLVKREKPIQENEILSEKDMIEEKIFMGLRMNKG
                     IKFEDFKKKFGIDFREKYNKQIEMLLARKLINQSFEGIQLTQKGREISNSVFIEFME"
     gene            82211..83230
                     /gene="hrcA"
                     /locus_tag="KR250_000683"
     CDS             82211..83230
                     /gene="hrcA"
                     /locus_tag="KR250_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcription repressor HrcA"
                     /protein_id="HBH0066648.1"
                     /translation="MELNERKLNILKAIVKDYIETAEAIGSRTISKRHDLGVSAATIR
                     NEMADLEELGYLIQPHTSAGRVPSEKGYKLYVNSLMSKSELDDNDKILIEQCMNHNIN
                     HIKELIHETSKLLSQLTNYTTVAVTKSLINQSVIKHIQLVAMNDNNILLIVVTDKGDL
                     KKANLTTNVYLDQSKLNLISDNLTRKLLGKSITDLDDNLIAFIKYEISEYSGLIDELL
                     NALNSNMKEEDFSLSLNGATNIFSYPEFNDVLKAKSFLNMLEKKETIADIIKSKGIQK
                     DNLNIIIGSDNDCELAQDCSIVTATYNVDRDLVGRISFIGPTRMDYARIYSIINYMSL
                     LINRK"
     gene            83260..83880
                     /gene="grpE"
                     /locus_tag="KR250_000684"
     CDS             83260..83880
                     /gene="grpE"
                     /locus_tag="KR250_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HBH0066649.1"
                     /translation="MDKKNEQQEVREENDTSINQESETQVELEEEVVNEECETSSEKT
                     DEKEVDDENVTDINSKLAEKKLQDELDELNDKYQRLQAEYANYRRRTQQEKETIGVFA
                     NEKIITELIPVIDSMERALDACEDKEDTMYKGISLVHKQLIDTLVKFGVEEIEAESKE
                     FDPNLHLAVMQESVDGVEANQIVMVLQKGYKLGTKVVRPSMVKVSC"
     gene            83972..85819
                     /gene="dnaK"
                     /locus_tag="KR250_000685"
     CDS             83972..85819
                     /gene="dnaK"
                     /locus_tag="KR250_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HBH0066650.1"
                     /translation="MGKIIGIDLGTTNSCVAVLEGGEAQIIANSEGMRTTPSVVAFTK
                     DGERIVGEPAKRQAVTNADKTITSIKTHMGTDYKVNIDGKSYTPQEISAIILQKLKSD
                     AESYLGQTVTEAVITVPAYFTDAQRQATKDAGRIAGLDVKRIINEPTAAALAYGMDKL
                     DQEKKILVFDLGGGTFDVSILEIGDGTFEVLATAGNNRLGGDDFDQIVIDYLAEEFKK
                     AEGVDLRNDKMALQRLKEAAEKAKKELSSTMSSNINLPFITATAEGPKHLNIDLSRAK
                     FEELTRGLVEKTMEPTKTALQDAGLSTGDIDDVLLVGGSTRIPAVQEAVKKFIGKEPH
                     KGINPDECVAAGASIQAGVLAGDVKDLLLLDVTPLSLGIETMGNVMTKIIERNTTIPT
                     KKSQIFSTAADNQTAVDIHVLQGERSMAYDNTTLGRFQLTDIPPAQRGIPQIEVTFDI
                     DANGIVNVSAKDLGTGKEQKITITSNTNLSESEIEQKIKEAEMNAEADKQKKEKIEAF
                     NQAESTIYQTEKTLNELGDKISSGEKEDIEKAIADLKAVKDNQDATAEELKKATDEVM
                     TKFQKVSQEMYQKAAQEQQAAQGAEQAQDNGPKDDNVVDADFKEVDEDK"
     gene            86030..87184
                     /gene="dnaJ"
                     /locus_tag="KR250_000686"
     CDS             86030..87184
                     /gene="dnaJ"
                     /locus_tag="KR250_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HBH0066651.1"
                     /translation="MSTKRDYYEVLGISKGAEAQEIKKAYRKLAMKYHPDRNPGDKEA
                     EEKFKEINEAYEVLSDDTKRKTYDQFGHDGLNGQGGFGGQGGFGGQGFGGFEDMFGDI
                     FGDMFGGSFGGGRARRRGPQRGADIRQSVTISFEEAAFGKKMSIKVNRSEECEECNGT
                     GAKPGTSKKTCSTCNGTGQVRTVQRTPFGNIASSRPCSACNGTGEVIESPCSKCHGTG
                     NTRKVKTIEVDIPAGIDDGQMIKLSGQGEVGEKGAPRGDLYIVVNVKSHPLFTRDGND
                     IYFEMPITFVQATLGDEIEVPTLDGKVKYSVPEGTQTGTVFRLKEKGIPRIRGNSRGD
                     QYVKVVVEIPKKLNDKQKELLREFAKECGSNVHEKKKTFGQKIEDMFKKK"
     gene            88150..89085
                     /locus_tag="KR250_000687"
     CDS             88150..89085
                     /locus_tag="KR250_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBH0066652.1"
                     /translation="MYYIGVDIGGTGIQAGVVDNYGKIIFRSECKTVIEKGFEGILND
                     IKIMIYKLLEDNKLTMSDIKSIGFGVPGFINKEGLVTCVNLKWNKKAFNKELKKRFPD
                     VEIHGENDATVAALGEAKFGSMKGANVGVLYTLGTGVGGGIVINQKVFSGAHGLGSEI
                     GHQIIGENYFNCNCGNNGCVETFCSATAIIKYSQKLIEEGEKSRILDLAEGNLENVNA
                     KMVFDAYRENDLVAIKVINRFKEYLAKTFANTINSLDPEIISIGGGISKSSDIILDGI
                     EGLVRKFVLYKTEDIATITCATLGSDAGIIGAAFL"
     gene            89121..90494
                     /locus_tag="KR250_000688"
     CDS             89121..90494
                     /locus_tag="KR250_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBH0066653.1"
                     /translation="MGKTNARRTLFLIAIGSGTMLNPLNSSMISLALHSIQNDFHISF
                     TTVSWIVSAFYLASAIAQPVSGKIGDLIGRKVLFLSGLMLVILSASIVPLVQSFFILI
                     FIRVIQAIGTSTLYPSGVALIQNNIKERQSSALAVLTIFASTTAGLGPTLGGLLLDLG
                     GWHAIFLVNIPVVLVSICLGYFLFPKEVKEKKSIKETLKNVLSRLDIIGILLFSIAMI
                     FILLFLLSMKESFNLEQLVFGIILMCLFIWHELRTKLPFIDIKLFVSNPKLSKVYLQF
                     IILNFFNYILFFGLPSYFQDALHYSAKSTGLFMLFMSGIGIFISPLTGKWIDKSGTRF
                     PLVTSSIFMFISAVLLSVVFIHIPVIGKGIILSLAGISYGVGNVALQSSMFEESPRDS
                     IGTASGLFQTSRYLGSILATVLLGMVFEVNITSEQFQILGYVMVVLGTISFLLNFILK
                     KELRKVE"
     gene            complement(90682..>90915)
                     /locus_tag="KR250_000689"
     CDS             complement(90682..>90915)
                     /locus_tag="KR250_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH0066654.1"
                     /translation="DFNKIKSIDEELLYTGIKFINNDLNISKTSTSMFLHRNTLVYRL
                     EKINEILGFDLKNFENAMIFYLSVKSYFLYKKI"
BASE COUNT        35380 a        10202 c        15406 g        29927 t
ORIGIN      
        1 tcaatagtta caaaggttca agaatttgga aaacttagag caattggtgc aactaagaaa
       61 caaataaaaa atatagtgtt taaagaaggg ttcatattag ccggaatagc tataccaata
      121 ggaataattt taggctatgt acttgcaaat attataataa aatcatttat ggatatagat
      181 gtaaaatcat cacgattacc agttatatta tcggttgtgg taataagctt tatatctgtt
      241 gttttatcac ttttaaaacc aatgaaagta gcatcaaaag tatccattgt tgacgcagta
      301 agatatacag gaaataaaat aagtaataag agtaagagaa aaggatataa aaatataaat
      361 ctaaagagat tatcacatgc caatttagaa agaaataaaa aacgtactta catgacattg
      421 gcatcactta tacttagtgg aactatattt ataacagtat caactgcctt agaaagtttt
      481 gatgctgaga aaatggcaag agaacacttt ccttatgata tagaagttag actatctggt
      541 tatgaaatga acagtgataa aaatcctaaa gataatttga atattttaca aatggataat
      601 cctctaagta aagatttttt taatcaaata aagaacatgg aaggaattaa aagaatagaa
      661 tctgcaagaa gtgtaaaaat tggtatggag gactatgatg ttgaatttaa gtatgacctt
      721 ttacaaagta taaatgaaaa tgatgtaaaa tcattaaaca agaatataat agatggaaaa
      781 ataaatcttg aaagacttca aactggagat gaaatagtaa ttactcatgt tgatacagca
      841 aaagaaatgg gagtaaaagc aggagataaa ataagattaa ctttatatga tggagataaa
      901 aagataaaaa aagaatttaa agttcaagca atagctatgg gggttccaag ttttggtata
      961 gggaaagatt ttatagatag aactttgaaa tacgattcaa ctagtagctt gggaatatat
     1021 acagaagaag gaaagtatca agaaatcaaa gatagtatca agaagattgc taaaaataat
     1081 ggatttttag agactgattt tatagatagt agaattgagt caaataaggc tactatttca
     1141 tttataaaga taatgggata cacgctaaca ggcataatag gtgtgattgg atttatgaat
     1201 cttgtcaaca caatgataac tagcatagtt actagaaaaa aagaacttgg tatgcttcaa
     1261 gctattggtc ttactaataa acaattagtg aaaatgctta attcagaggc aatatattac
     1321 acttctggaa tgatgatagg aagtattcta tttggaggta tattagggta tattgctgtt
     1381 atatttctaa aaaagactgg attatcttat gcaacatatt ctttgccaat agtaccaatc
     1441 ttgttgatga ttgtgtgtat tcttatagct caatttataa caacttattt gataggacga
     1501 agttttaata aagaatctct tattgataga gtaagataca gtgaataaaa tgcaaatatt
     1561 tatggttagt ttaactagca aaaaacatat aaaaaaatat agttaaagag attcataatt
     1621 aaataggtta aagcatagta cagagcaaac aatttaaaca gatgtttgct ctgtatttta
     1681 ttatgtaatc aataatatat ctaatatact gatattattc atgttagaaa atgtaccggt
     1741 acaattctaa aaaagtataa aattgtacca tataaaatga aaattttgta taataatcgt
     1801 atagaaaata aataggagct gatatatatg aataagaaga ttaattatgc accaggacca
     1861 actgaaacta gggaaaatgt aagattagca agagcagaaa aaacaacaaa tccagatata
     1921 gatattgatt ttgttgaatt ttataaaaag acatgcgaga agtttggtaa tataattgga
     1981 acaaaaaacg atgtttacat attgagtggt gaaggaatat taggtcttga agcagcatgt
     2041 gcatcactta cagaaaaagg agatagagta cttgtaattg acaatggtat atatggagaa
     2101 ggttttaaag attttgtaac aatgtatggt ggggagtatg tgcttttttc aagtgagtat
     2161 actaaaagta tagatattga tgaattgaaa gcatttttag atgaggatag taattttaaa
     2221 tatgcaacag tagtgcattg tgatactcca actggcgtat taaatgatgt aagcaaaata
     2281 tgtccattac ttaaaaaata tggaatatta actgtagttg attctgtagc aggtatggta
     2341 ggagaaagat taagtgtaga tgaatctaaa atagatataa tattaggtgg ttcacaaaaa
     2401 gctatttctg caccagcagg acttactata gtcggaataa gtcaagatgc aaaaaattgt
     2461 ataaaaaata gaaaaactga tgtaataggt ttttattgta atttaaatat atgggaaggc
     2521 tattatgaga aaaaatactt cccttataca atgccaataa gtgacataat ggggcttgat
     2581 aaagcacttg ataatatatt agaagaagga atagaaaatg tattaaatag acatgaaaag
     2641 atagcttata gtgttagaaa atctattgag gaatatggtc tagaactatt tttagaagaa
     2701 ggatattcta atacagtcac agctataaaa attcctgaaa gtataggagc tttagaactt
     2761 actgattata tgcttaaaaa ttataataca cttgttgcaa catcactaaa tcaatatatg
     2821 gatactattt taagaatagg tcatatggga gagaatgcaa atctaaataa aatagaacat
     2881 gtactaaatg ttttggataa aagtttaagt gcattaggat tcaaaggaaa tggaaatttg
     2941 ttaaatttat ttaataagta ttatttttaa tagatagggg gatatagtat ggaagttaca
     3001 gctaagcaaa aatttacaac gaaagacatg gtggaaacct cactattaat agcattagta
     3061 tttattgcaa ctaaatttat aaacataaga ttaccaatat ctataaatgg tgggcttgtg
     3121 catttaggaa ctgcaatgtt gttcatatca gcaatagtat ttggaggtaa aaaaggtgct
     3181 ttatcagggg ctattggtat gtcacttttt gatttgatat ctgggtggac tttatgggca
     3241 ccgtttacct ttatagttag aggagttatg ggttatttac ttggaaagat tgcatgggca
     3301 aatggtaata atgggaataa ttttttaatc aatgtaatag gaatatgtgt ttcatcaatt
     3361 tggatgctat ttggatatta tgtaacagaa gttatacttt atggtaattt tatagtacct
     3421 cttacttcaa taccaggtaa tttaatgcaa gtattgatag gtcttataat agcgcttcct
     3481 atttctaagg tattgaaaaa atgtataaaa taaatataaa gtgaactaaa atttatgata
     3541 gcataaatta tactgtataa tattaggatg gagtacataa ttaaaaaatt ttagtataga
     3601 ggttagttca caatgttatc agttacaaga tattcaatca aaaatgttcg tttaaagccg
     3661 tgggtaaagt ttatttggta ttttgagaca aaatcaaata tactgttaaa taataagtta
     3721 cttccgacag acagtataga tattattctc aatctttctg atgttatgga atataaaatt
     3781 gaaaatcaag actatactgc tagtaatatg cattttaatg gaataaggga taaacatgga
     3841 tttattattc aacatggaaa tatacgtgtt atagggattt ctttttatcc atttggtctc
     3901 tatccatttt taaagatacc aatttcagag tttaacagac aaattgttga cctagaagca
     3961 gtatcacagc tatttgcaaa aaaattggaa gagtcattaa atccaacaca atcagtagaa
     4021 aaaattgttt tatatttaga agaagtatta ttatccatat tagaagaaga tttaatttca
     4081 aataaatatg taaagcttct aaattcattt atatatgtta atagatatag caatataaaa
     4141 gcattttgtg atgatacaaa tataaatatt aaaacattgg aaagaatctg tttaaaatat
     4201 acagggtata cacctaagat tttaaagaga atttacagat ttaaaatggc aagcaatcaa
     4261 cttatttata attataaaga tgatgaactt tttgatttta tttatgaaaa tgagtactat
     4321 gaccaagcac actttataaa agaatttaaa aaattttcag ggacgtcacc aattaaattt
     4381 attggtgaga ataagacgat taaagaaaat ataaagtaca gctatttata attatactgt
     4441 cgatttttta caatacctcc aatttatttt ttgatatgat ataggaaaat aaattggagg
     4501 ttttacatat gaaaattatt acagaaatag ttgagtttaa tgtggaagaa aatttatcaa
     4561 aagaagagtt tatagaaatt gtaaatgact tagaattaaa atttcattct attcaatctg
     4621 gttttattga tactgagctt ttatatgatg aaaagtctca aaagtggatt atgatacaac
     4681 attgggaatc tatagaagac ctcaagagtg cttctagtaa aatgtttaaa gattcttcaa
     4741 ctgagaaatt tagaaaagct ttaaagaatc aaactgttaa gatgaatata ataccacaaa
     4801 ttaaaagttg gaaattatag aacttaatgt aaaagatagt taaatagtat caaactaaat
     4861 tagccaaata acattttgta tttaaattgt tatttggctt ttagtgttta ttttaatatg
     4921 agtgttatat actagaaaaa ccaaccttaa atactactat ctaaaagcac tattgctgta
     4981 cgagaggcaa taggcatttt agaagaagga ggagttgagc taaagtatac atcataatct
     5041 cttaagtttt ctttttttgt gtttcctcca aactctacca tatcagtatt taatatcgtt
     5101 ttgtagtttg tattttgaaa ctttgaaata tcaactgatt gtattagatt agggtggaaa
     5161 ttaaatataa ataaatattt gcccttactg tacaccaaaa tctttttatc attatccata
     5221 tgtataagtt catgactatt ttggataaaa atatcactgt attttgtaag ttcgagcata
     5281 gatttatcaa aatttaataa ttgtttatac tttaagttgt tatcttctga aaggctccat
     5341 tgtcttctgg catacttata gctccaattg ttgccttctc gtggaaaatc aatccactca
     5401 ggatgtccaa attcatttcc cataaaattt agatatcctt cacctgctag agaaaaagtt
     5461 attaatttaa ttaatttaat aagagatatt gctctattga taacatggtt atttgagttt
     5521 atttccatat tccagtacat ttccttgtca gctagccaaa aaataattgt tttatcgcca
     5581 actaatgctt ggtcatggga ttcacaatat cctatatttt tttctccagg tcgtctagta
     5641 gtaagttcat accacatttt tcccaaatcc cagtcttcat cagataagtt agagatagtt
     5701 tttatccaaa aatcaggtac tcccattgcg agtctatagt caaatccaat tccgccgtct
     5761 tttataggga tacacatacc aggcattcca ctcatatctt ctgcaatact tatcgaatta
     5821 ggtttaattt cttttattaa ttcatttgca aattggagat atgttatagc ttcaatatct
     5881 gtattcatac taaaatattt ttcataacta tcaaatgaaa ctccaagacc atggttatga
     5941 tataacattg aagtcacacc atcaaaccta aagccatcaa aatggtactc atttaaccaa
     6001 aattttatat ttgaaagcaa aaaatgtatt acttcaggtt taccatagtt aaaaagtttt
     6061 gtaccccaag caggatgatt tccctttgaa cctgagtgga aaaattgatg ttcacttcca
     6121 tcaaactcat ttattccttc taaagtattc tttacagcat gagaatgaac taaatccaat
     6181 aaaacagcaa tccccattga atgagcagtg tttatgaggt tttttaagtc ttcaggagta
     6241 ccaaacctag atgagattgc ataaaagttt gaaacttggt atccaaaaga tgcataatat
     6301 ggatgttcca taatagccat aagctgaatg gtattgtatc cagctttttt tatcttagga
     6361 agtatttttt cagtaaattc attataagtt ccaatagatt cgctttcagt agacatacct
     6421 atatgacatt catagattag tggataagtt atgtttttta agtcaaaatc attgtcagtc
     6481 cagataaatg gagtttttgg ttgccatatt tgtccattaa atccaccaaa atctttttga
     6541 attactcttt ttatatataa tggtatacgg tcaaatgttt ttccatttgc agtaacttgt
     6601 acctttacct ctgatttgtg aggtaagcta ttttgaccag gaataaatat ttcccaatta
     6661 ccagacccaa tttttcttag aggatgagat tttctattcc aattattaaa gtcaccaatt
     6721 agtgacaaac tatctgcatt tggagcccat tccctataga agaatccatc atttgtctga
     6781 tgaaatccat agtaaagatg agcatttgca aaagaactaa aatctttata gtttttcaat
     6841 gtttttgatt tcatattctc ataatgtttc attcgctttt ttatatcaga ttcaaatggt
     6901 tgtaggtata catcaatatc taaaatttta aatctttttt gacttttcat tgtatttctc
     6961 ctccataata aatattacat ctaaaaaata atatttatct catactatct tctctatata
     7021 attgatatat attatataat cctaataaaa agcttaagcc accaatgatt aatttcataa
     7081 tttacctccc tagtatttat acttatatat cgtattcatc aaaatttaaa agtttgttta
     7141 aaactctttt aataatatca taatcaaagc ctttatagga aagatgttga gaaatttttt
     7201 gatatatttt ctttttatct tctttcttta ctctttcata cctttttttt gctagataca
     7261 tagcattttc aaattcagtg tccttgtcta attcagatac tgcttcattg attgtacttc
     7321 tattgatacc tttattatat aaattctgct taattctgtt tttgccacat ttatttaggt
     7381 taacatttgt gttaacaatt ttttgagcta atacactgtc atttacgaga ttataacttt
     7441 taagaaaatc tatgaccctc tcaattatat tttcatcaaa ttctgatgat agtttttctt
     7501 ttaatttttt ttcagattgg tcagcatgag ataaaatatt taatgcttta tttttagctt
     7561 ttatatacat ttcatcatct aaaataggtt ttaattcttc ttcatttatt tcattcccct
     7621 tttttaaatt aaatgtataa acaagttctt taaatatagc aataaaaaat ttatcatcta
     7681 cataaatatt aactctatca tcatttcttt tttgttgttc tatttttgta attataccca
     7741 taagtcctcc ttttttatat gtaattatat taacaaaagc aactaataaa atttaacaaa
     7801 aaacttatat ttgtatgtta tattttatca aaattaagac aaaatagtaa aaagttattt
     7861 ttattaaaaa gtaaagtaga atcatgttaa ataattattt ttttaagtgt tttttagcat
     7921 ataaatataa ttatataccc aaaaaaacaa attataagct ttaagaaata aaaaataatg
     7981 gtacaatagt attataacat taaaaggaga caaaatatga gaagtgaaaa tctagttaag
     8041 atggcagagt tgaaaaatac tgaaaaacta gagaaattta atcgccataa aggcaaaatc
     8101 aaaataggta tattgggagg tacttttgac cctatacact atgctcattt agcaacagca
     8161 gaatttatta gagacaagta tgacattgac aaaataatct ttataccatc aggaaatcca
     8221 ccacataaat tatgtatcac tacagataag tatgatagat ataatatgac acttctagca
     8281 actgaaagca acgaagattt tttagtttca aaagtagaaa tcgaaagaaa gaaaaggact
     8341 tatacaatag atacattaaa atacttaaag aaaaaataca aaaatgcaga catttatttt
     8401 ataacaggtg cagatgcaat ttgtagtgtt gaagagtgga aagatgtaaa gaaaaacttt
     8461 gagttagcta cttttatagc cgcaactaga ccaggaatta gtttgttaag gtcacaagag
     8521 actatagaaa aactaactaa gaagtataat gctgacatta taacagttta tgttccatca
     8581 ctggatatat cgtccacata tataagggaa cagctaaatg aaggcaagtc aatacgttac
     8641 ttagtacccg aaaatgtaga aaattattta tatgaaaata aactatatca atatggagat
     8701 gattaaatga acttagaaaa tattaaccaa agactaaatc aaatgctacc agtggggagg
     8761 cttagccatt caaaaaatgt tgcaaaatgt gctgaaaaat tatgtgagat ttatggatgt
     8821 gacaaagaaa aagcttactt agcaggaatg attcatgatt gtgcaaagta tttaagtgac
     8881 aaggaaatag aggattatgt aaataagtat gaaatatatt tagacccaat agaggatgga
     8941 aatcgttcct tgtctcatag cgtaattggg gcgtacatat gcgagtatga atttgaagta
     9001 gaagatgaag atattataaa tgcaatcaaa tatcatacaa cagggagaga ggacatgtct
     9061 cttttagaaa aaataattta catagctgat ttaattgaag aaggaagaaa attcccagtt
     9121 gtagatacat taagagaatt agcatatgga gggaagcttg atgaagcact tttaacttct
     9181 tttaataata ctttaatgtt tgtcatcaat aaaaaagaag agatacatcc aagaacagtt
     9241 atggcaagaa actatctaat taaagaaaag ttattataaa aaagctatat aaaggcaaac
     9301 atattaataa atttaaattt gactatgtta caaaatcgta ttaatatgtt acaataacta
     9361 agtgtatttt aattaggaaa gtgaggaatt tttatatgac agtggaacaa atgacaaaaa
     9421 tagcatatga tgccatagaa gataaactag gtcaagatac agttataata aatataggaa
     9481 aagtttctag tttgtgtgat tattttatta ttactacagc atcttctcag agacaggtaa
     9541 aagctattgc tgacaatgtg gaagatgaat tagcaaaact aggattagag ccaagaggta
     9601 aagaaggaca aggaactcaa acttgggtac tccttgatta tggagacata atggttcatg
     9661 tatttaatga ggaaaataga gggttttata atttagaaaa attatggaaa gatgcacctt
     9721 atatagatat tgacacatta gcataaacat gataatatat gttattataa ttagaaaatt
     9781 tcaaaaatta tattaaattg aatagactag agcagtaaaa gattatattt tttcagagag
     9841 ttagtggaag gtgagaacta atattatata actttgaact tactagatta aaatctattc
     9901 tccatggctg gattaaaagc tatctaagag agtctatata tgtagtaggc taattagggt
     9961 ggtaccgcga agatttatcc tcgtccctaa acgtaagttt agtgacgagg attttttatt
    10021 ttcaaagaat tatttggaaa ttataagtaa ataatactaa ataaggtagt tatacacaat
    10081 taaaaatagg aggcaaaata tgagcgtata taattttaaa gaagtagaat cgaaatggca
    10141 aaaaatctgg aaagataata atcagtataa aatggatact gcacaaactg aaaaacctaa
    10201 ttattacaca ttagaaatgt tcccttatcc atctggaaaa atacacatgg gacatgttag
    10261 aaattattct ataggtgatg ttgtcgcaag attcaaaaaa atggaaggat ataatgtact
    10321 tcatccaatg ggatgggatt cttttggatt accagctgaa aatgcagcta taaaacatgg
    10381 aatacatccg cataaatgga caatggaaaa tatagaagaa atgaaagagc aattaaattt
    10441 attagggatt agttatgatt gggataaaga agttgcaact tcaactccag aatactatag
    10501 atttacacaa gaaatatttt tgaagttttt agagcatggg cttgcatata agaaaaaatc
    10561 atatgttaac tggtgtcctt cttgtgagac tgttcttgca aatgagcaag ttgttcaagg
    10621 agcatgtgag agatgtaaag caacagtatt aaagaaagat ttagaacaat ggtattttaa
    10681 gactactgag tttgcagaag aattacttaa tgacttagat acattagatg gatggccaga
    10741 aaaagttaaa acaatgcaaa aaaattggat aggtaaaagt acaggagcag atttagtttt
    10801 tgatatagat ggaactgata aatctatgac agtatttaca actagaccag acacaactta
    10861 tggagttact tatatggtac ttgctccaga gcatgaatta gttaaagaat tggttgcagg
    10921 gactgaatat gaagcagatg ttgaagcttt tgttcaaaaa atgcatacta tgacagaaat
    10981 agaaagaaca gcagctgatg tagaaaaaga aggaatgttt ataggtagat atgttataaa
    11041 ccctttaaat ggcaagaaag ttcctctatg gatagctaac tatgtattag ttgagtatgg
    11101 aacgggtgct ataatggcag ttccagcaca tgatgaaaga gatagagatt ttgctgaaaa
    11161 atataattta gacataattg atgtaataac agaagataac aaaatgataa attcagaaga
    11221 atttaatgga ttagatgctt cagaaggatt tgaaggaata atcgataagt tagaaaaaga
    11281 aggtagagga aagagaacaa ttaattatag acttagagac tggttagttt caagacaaag
    11341 atactggggt tgtcctatac ctgtggttta ttgtgatgaa tgtggaatag ttccagttaa
    11401 aaaggaagat ttaccagttc ttttaccaac agatgttgaa tttactggaa aaggggaatc
    11461 tccacttacg acttcaaagc aatttatgtc aacaacttgt cctcactgtg gaaaaccagc
    11521 tagaagggaa gttgatacta tggatacatt tgttgattct tcttggtatt tcttaagata
    11581 tgtggatagc aataatgaaa atgaaccatt tagtaaggaa ttagttaata gatggcatcc
    11641 agtagaccaa tatataggtg gagtagaaca tgctataatg catttacttt atgcaagatg
    11701 gtttgtaaag gcatttaaga gtatgggaat ggtagacttc aatgagccat ttaaaaactt
    11761 acttactcaa ggaatggtac ttatggatgg ttctaaaatg agtaagtcta aagggaatac
    11821 agtatctcct atggatataa tagatgagta tggagcagat actgcaagac ttttcgtatt
    11881 atttgcagca ccaccagaaa gagatttgga ctggtcagag caaggtgttg atggatgctt
    11941 tagattctta aatagggttt atagattggt tgacgagtta gcagatgtgg ttaaaaagga
    12001 tgtagagttt ggagaattaa attcccaaga taaggatatg agatatacta tacactctac
    12061 acttaaaaaa gttactgctg atttaagtga aaaattcgga tttaatactg ctatatcagc
    12121 tcttatggaa ttaataaatg atatgtataa atataaagag ttagataata taaacgaagc
    12181 agttataaaa gaaggtgtac aaactatagt aactataatt gcaccatttg cccctcattt
    12241 aggtgaagaa ctatggacta tgataggtaa agaaggaagt gtatttgata tagactggcc
    12301 taaatatgac gaaaaagctt tagtaaaaga tgaaatagaa gttgttgtac aagttaatgg
    12361 aaaagttaga ggaaaattaa ctgttaactc aaatatatct aaagatgaga tggaaaaggt
    12421 agctttagaa gatgaaaaaa taaaaggatt agttgaaggt aaaactatag ttaaagttgt
    12481 agctgtacct aaaaaattag taaatatagt tgttaaataa ctcaaaaata tgaacaaaaa
    12541 tataaattta taaaataaat gaggctgtct caaaatagag ataagccttt tttcattgtt
    12601 gaaattataa aaatagattt gattttatac aaaaaaatga ttaaagaact aaaaagttca
    12661 ttcttatata ccctagttgt tgttttaaat attattataa ttaatgttaa atcgagttaa
    12721 tgtattaagt ggtatgtaaa tcattcagtt tccaaagtgt tgttcgtcaa ggaactgctc
    12781 taagcttaaa cgaaaaaaat ataattattg tattttgaaa tttttttcta tataatgaaa
    12841 acaggcaaaa aaggtgcata tctatgcttt atatgaaaca cccagacaac gaaattcaga
    12901 ttcatataat agctaccatg tttctaaaaa ggaacttgta aaaaagatag cgtttcaact
    12961 tcccagttga ttaaaattat cagcaggaaa acaagtatgg tcagcagtgg tattctaatg
    13021 ggtgttgtcg atatatccaa gttgggattg tctgaggatg ttatcaaata acttgtgata
    13081 ggtgcagtag atgtggagat tcctgatacg gatttattct caggggatgg ttgcttcggg
    13141 agatatggag cgtaaccagt ttattgatat ggtaaccatt ttttgtctaa tttgatgaaa
    13201 gaatcccaag aataccatac agaagtaaga caggaattaa ctggtggtat aaactgataa
    13261 aggcaaattt aaaaattttg ataggatgat tttaagattt atcttctatt ttgtttttta
    13321 gaaataattg aaatgttatg aggaggtatg tacaaatgga gactgtaaca aataatcaat
    13381 caggtgtaga aattcaactc agtctgtttg aagctatcaa agatttattg ttaattaatc
    13441 ctattttctt gttgggaatt atcttgattg gtattttctt agttgtaata ttcaagaaaa
    13501 atagtgatat tccaaaggtc aaaacatcta ttttatcact ggcaatgtat tactacttat
    13561 gtttaacact atcgcatatt gttggtatac ctacattcgg tgaatatata agactgtcac
    13621 agttagggga aacgttcttt aatccaaaca taaatcttat tccttttagt gatggattta
    13681 gtttgagttt tattttgaat attttacttt ttgtcccatt aggtttttta tgtccgctta
    13741 taagcaaaac ctttgaacgt ttgagaaata cattcttgat tggttttgga ttatctcttt
    13801 ttatcgaaac tattcagctt tttacgttac acagagcaac cgatatagac gacttactca
    13861 cgaacgtaat tggaacaata ataggctatt tctgcttcaa actgattgct aaattgcgaa
    13921 ttgtaaaact acattcagat gatgactcta tggaacagga ttacacagta tgtattccga
    13981 ttgccattat aatagttgcc cttatattag gattttttag ttaagttcca aatagctaat
    14041 ttgggaatcc agacaatata acagatacta tgttaccttt tacaggagca tactatctgt
    14101 tatattgtca acatcctatt ttatggtttg tgtgtagttt cctgtaaact gaactaaata
    14161 attaaataga agctattaga attaataata acagatagat attaataagc ataaacaaat
    14221 agtaaaatgt gtagatatag acgtttttaa gtgaaactat tataaaataa ttgactgata
    14281 atcaactgac aaaacctatt tttttaggtt taactgacat ttaactgaca gtcagttttt
    14341 actatttaaa tagaagttcg cttagattgt cggaagcttc tttgtccatt tctttaagta
    14401 catgagaata tctattcata gttattttta tatctgtatg acccaatctt tcagaaacaa
    14461 ctttcatatt agttccccct aaaagcagca gagtggcact tgtatatctt aaatcatgta
    14521 ttctaatctt ttttaaatta ttttttttgt aaaattgtga aacgtttttc ttagtgaaga
    14581 aggtagaaac ggatttaaat tggtgtttag acaaactaag ttatttctgt tttccaggat
    14641 accttacaat tttaatttgc tttgtcttag tttttcaatt ttcaattttt caattaattc
    14701 ttttggagct gataaagctc tttttgattt tggtgttttt ggctctttaa aagatatttt
    14761 gccattagaa tagacaagaa tttgattaac atttatcaga ttgttatcta agtcaacatc
    14821 tgaccaccta agcccaatag cttctcacat tcttaaacct agaagtaaca ttaaaagaat
    14881 aggtatttct atattattac caagtaactt ttctattagc aataatactt cttctctatt
    14941 gtatatttca tttgtagaat atttttcttt agaaggaagt tttataaaat cacatgaatt
    15001 ttcttgtatt tctcttaatc tatacgcttc ccttattaca gaacttagaa agctatacct
    15061 tactttagca ctagtaaagg tagaattatt aaagttataa tttacaaaag cttgtactga
    15121 actaggattt acttctgtga gttttagatt cttaaaaaag gttctatgta atttttaacc
    15181 caagattttg tatttgtaac agtatatgga gaccaattat ttttattcat gtctatatat
    15241 ttataacatc tttctacaaa tgtcatacct tttggagcaa caaacttatt attatttata
    15301 gtagacttta tttcaattaa atgtttttca gcatcctttt tctttttata gcttccataa
    15361 cttttttgtt tctttttgcc agtttcttat tccacatata catagaattt ttcccatctt
    15421 tttcttataa aagctgattt gatattcata tatgtacgct ccctttataa gttagtttta
    15481 taaaagaaca gttggactgc tctaattatg tcattatctt tttaattctt tacagttaca
    15541 ttataacacg tatttgtata cacttaaatt aacaggaaga taaatatcgt taaaggagga
    15601 aaagaaaatt taatgtagaa ttatgtaaga tatatagtat ttcatatata aaagggggag
    15661 atattgtgaa attaaagaaa ttattatcac tagggttagc cgtgtcaatg tttgctgctg
    15721 gtcctatatc agctaatgca cttgatacaa tttatactat tcaaggaaaa gataaatatg
    15781 aaacggctgc tcttatagct gatgaacaaa attatactac agctatatta attaatgcag
    15841 acagcacaat ggctgatgga ttaagtgcga gtggacttgc aggagcaaca aatgcaccaa
    15901 tttttttaac taagaaaaat aacataccta atgcaactct taaaagagta gagaaagcta
    15961 aaaaggtata cataataggt ggagaaagtt ctattgataa agcaacagaa actcttctaa
    16021 aagataaagg aatagaggct aaaagacttc aaggtagtga tagaattaag acaagctata
    16081 atgttgcaaa agaaataaac tcaattaaca aagtaaataa agttatattg actaatgctt
    16141 ttaaaggaga gcctgatgca atgagtgctg cacctgtagc agttagagat aaagcagcta
    16201 tagtattaac tgatggaaaa agtgttccat ttaatacaac tggtgtagaa agttatgtaa
    16261 taggtggtac ttcatcaatg agtgacaaac tagttaatga tactaattca actagattag
    16321 gtggagtaga tagatatgat actaataaaa aaatagtaaa taagttctat aatggcgcaa
    16381 aagaatttta tatagctagt ggtactgatt tagtttatgc tttagttgct tcaccaatgg
    16441 ctaaaaataa tcctattgta ctagttgata ttggaagtaa taaagatata cttaaaaatg
    16501 ctgcaaaagt gactgggata ggaaatctaa gtgataaaat tatgctagag tgtgaaaata
    16561 ccatcaataa tattagtctt ccgtcttcaa ataaaaatat aatatgtgga ttaaagattg
    16621 attttgattc aaagcacata atttggcaag ataaaggaag ttatcttgaa tgttatgatg
    16681 ataaccttga atattatact gaaaaatata aagacccaac agttcctata aatatagaaa
    16741 atacaagtac acaaaattta gatgtaacaa aattaaaagt ttatggagaa gctaattcaa
    16801 cattatatat ggggcaatat gctaaaataa aaatggattt taatagtttc ggtgtattaa
    16861 agccaggaga aaaatgtact atttatttag atataatttc attagatcca tcaagaagca
    16921 tgacattaac attttcttat gtaggtgata aagaaaatta taaatatgaa caaggattgg
    16981 caagtagaat attattttat aacccagatg gagaagattt ttttgaatat aaataactag
    17041 tgggtatgta aattaataag ggttttataa gtttaatttt tttattttcg ttttatatta
    17101 actatgtggt atgtaaatgt ctcgatattg ctcatatgtt gagttattat atttagtttc
    17161 atatgaacta tatggatttt aaaattaaaa atacttaaaa acatttactt taatagtagg
    17221 tgtttttttg attaaaagaa tattattata atgtaaaaag taataaatag gtaaaatatg
    17281 taagaattat atgttataat atttttagca agaagatgta atctacaatt tatagagtgg
    17341 agttcatgta tcaaaaaatt atcctcccga cgctaagaag ggaggtgaat atacatggat
    17401 aattttttac aaggcatact agcaagtcta tctgctagct taatagttta tatagctagc
    17461 aaactattta gaaagcgtaa aaaaccactc aaagcggcaa ctaagagtgg ttgggaattt
    17521 gatttaaaaa tcagattcca taaaactaag taatttctaa attatgaact ccactctaca
    17581 gcaaaataga ttgtagttct tcttgctttt attatatcac aaattggtac agatattcaa
    17641 aaataatatt tttatgatat aataaagtca tagaaatttt gcagtgttcg attttttgaa
    17701 gaaattaagg cttaacagtt gaaatataag gcattgagga cttgtgataa gtgttatcaa
    17761 ttgcactact catggttcac tgcaaatttg agagatttgt atatgtgtag gcattggaaa
    17821 tactcaattt attttggggt tttatattaa ctatatggaa tgtaaatatt ttttttatat
    17881 ataaacttct aaacttttac aaaagtttta tattaactat atggaatgta aatacacttc
    17941 caacaagcat tgtagggttg aagtctacag gttttatatt aactatatgg aatgtaaatt
    18001 aaatcgtatt tataattata tctacactta gtaaatgttt tatattaact atatggaatg
    18061 taaatccaat agaccctcca ataactccag atattccttc tggttttata ttaactatat
    18121 ggaatgtaaa taatgaacta tctaaaccaa ggtcataatt taattgagtt ttatattaac
    18181 tatatggaat gtaaattgta tccaaaaaat tacaataatc atacgaattg ctaatataag
    18241 cagtcaatat atgtgtacat ttatccattt tatctctaaa catataaatt attatccccc
    18301 taaaaaactt tcataaaaac aaaaagaaca atatctcttt ggcaactggc taacataact
    18361 cataagatat ttatataagt cttagtccct atagctttgc gtcactagat ttctctagtt
    18421 ttgccgattt agttttattc tataactaaa taatacaata gaattagtta ttattcaaca
    18481 agattgtttg aaaattgaat aaatttatag gtatgaacgt aatttcacac ttaaaaacag
    18541 actaaatacg tagaatatat agaaaaattt acctgtagtt tttgtgataa aaagctccta
    18601 ttattaattt aagctagata agtttgagga agtgacataa attgaaagaa ggaatatatt
    18661 atgttgaata aaaaattacc agatgcagaa ttaaaaatta tgaaatacat atggaatact
    18721 ggttataaaa ctgtaatatc aaaagatgtt gcagattaaa tagaaaaaac atatgcttgg
    18781 aagcatacaa ctacaattac acttttagct agattaacta aaaaaggttt tttcatatcg
    18841 cagagaattg ggaaacatgt acactataga gtattaataa aagagaaaga atatctcaaa
    18901 atagagggaa aaagaatttt tggaggtcta cataacaatc cattatcaga actaatttca
    18961 aaattacatg atggagaaga aataactgaa gaaaaaataa tagaaatagg aaattggata
    19021 aaaagctgga aagatgaaga ttaataaata aaaggtagct actaatataa gtgctacctt
    19081 ttttcttgtg gataatattg gggataagtt gtggaaaatt tgtgtaaaaa ttgtggataa
    19141 aatattttgc tgacatttaa ctgacataag tattagattt tgatttaaaa atgttgaaat
    19201 atcaataaat aattaaatag aagttattag aattaacaat agattccttt ttcaaagaag
    19261 tctttgattt catcaaggtt atcagttttt ccaagtgcta agataagttt tattctagct
    19321 ttttgacctg gtaagtcccc accaaatata caaccaagat tttttaaatc tctaccagaa
    19381 ccaaagtaac cataactatc gaatactcta cctgaatgac atctagaaac tataactaca
    19441 ggaagtcctt tttctctagc atattcaact ccagaaagca tttgaggagg aatattacct
    19501 ctgcccattg cctcaataac tataccttta taaccactat caacagcaaa tcttataaag
    19561 tcagcatttt ctcctacata tgctttaaat aaggcaactt tagattctac cttatcagta
    19621 tctataattg ttctatttac aatatctctt gtaaggacaa gttcgttgca gtcaattatt
    19681 cctaatgggc cactagttag agatttaaat gtatttagag aaagagtatt tgttttagta
    19741 gcttcagaag ctaataatac ttcattgttt aaaactacaa ggactccttt tcccatagca
    19801 tctttggata ctgcagtaca aactgatgca gataaattac ttgaaccatc atatccaagt
    19861 tcagaactac ttctcatagc acctgtgact attacaggtt tgatgttgtc aattgtaagg
    19921 tctagaaaat aagctgtttc ctctaaacta tctgtaccat gtgttattac aacacctgta
    19981 atatcttttc ttgctaataa atcatttaca taatttttca attccatcat tctataaggt
    20041 gttatgtgag gtcctggaat ttcatcaaaa ttaaaaactt caacatctgc aactttatca
    20101 atgttggtaa ccattgatag tatttgttca cctgataaag ttggtatagc tgctccgact
    20161 ttatcatcta ctgtcataga tatagttcca ccagtaaaaa ctatggcaac ttttttctta
    20221 atactcatac tattctctgc ctttcataaa taaaatttat aattccgata ggattatatc
    20281 aaaaaaagta gttaaggtct acataaactc ttttattcat ataatttaga tatttcagtg
    20341 taatataatt atttcttaat tttttgccat tcaacgatat aaccatttaa tatctctctt
    20401 atggcttttc ctatcttagt ataagcttca tcatatagat ttgctagaga tattctaatt
    20461 gaccattcag ggccatagaa tccatttcca ctaagtaaaa ctactgaata gtcattagct
    20521 aatttataaa gaacatctac tggagatgca gttttactta taaatttaaa taagtcttct
    20581 ccgtagttta atttagccca ttcttcaata tcaaattgag tgtaatatgc agcattgttt
    20641 ttgttttcat taattggaag ctctaaagct tcaaataata gtttttgacg tctatggcag
    20701 atactcatat taagtttttt gtaggcatct actttgtcaa tcaatgcaaa agcagagaaa
    20761 aacgccattt gtacttgctg tggagtagaa agaccagcag tatggttgag agcaacaagt
    20821 ctgctatcag caacaactct ttccataaag gaaagactag aagggttaag actcatatca
    20881 ctgtaacgct tatctactga tttttttaat tctccagtta aatcatttat tttcttatca
    20941 aaaacatttt tcttatgaag ggcaaatgta ccaagtctcc atcctgtaac tccaaaatat
    21001 tttgaataag aatatactcc aacagtgttg taaggtagtt ttgacattaa ggatgaaaaa
    21061 tcttctacaa aagttccata tacatcatct gatattatca ttaaatcttt attataattt
    21121 tcaattacat ctattagatt attacaagat gtttcatcca tagctataga agcaggatta
    21181 ttaggattaa caacgaataa agctttaata gatttgtcac gaagtttctc taattctttt
    21241 tttgtatatt gccatgtatg agcacctttt tcatcaactt cattagcatt tatatttacc
    21301 actttaaagt cgtaacgagg aaggttaggt atttcaagat aaggtgtaaa tattggagtc
    21361 attaaggcta tggtatctcc tttttttaga agttcatttg ccattaatga atcaaatata
    21421 tagcacatag cagctgtagc accttcaaca gcaaaaattt ctacatcacc atatttacct
    21481 gatatatcat atttcatttc ttgaactagg taatcgtgta ctattttttc catatgaggt
    21541 aacattctat ctggaagtgg atagttatca ccaataattc catcagctag ttcatgaaca
    21601 aattcatctc catcaaatcc aagttcattt ataccaaagt ttataatatc tctagctaaa
    21661 tatgctccag gcatatctat attttcattt ataaatttaa aaaatctttc tttaataccc
    21721 tttttttgag gcattccagc caaatgacct aaatcccaat tagaacgagt ttcagtaata
    21781 gcgaaatgac caaaagtaaa aaaggcttct ctagcagttg aacaagtcca gtttggattt
    21841 ccacgacctg catcaagtaa ttctctgtta ttttcttttg cagtgtattt tgcaatatct
    21901 attaatttgt ccttaaattc aaaagggctt atttttccat atatgttttt gatttcttta
    21961 taagtatgat ttttcatgtt aaattcctcc ttatgtaata agtttcacat ttttataata
    22021 aaataaacaa gttgcaaaaa tgattatact ttaattataa taggtttata gaaaaagtaa
    22081 agtatgaaat taaaataata actctattta gccttattaa atatatgtta aaataaaaaa
    22141 taccttttaa taaaaaatga ttgttatata aaataaatat tcaatttaat tatcacctta
    22201 gtttttttat tgtattttat tatatatgta tagttttcta attttgaata aaaataaagt
    22261 aaaactttct tttatattga agttgtttag taagatatgt attttattaa tttattaatc
    22321 ttaataatgt taaataaaat caaaaaaata gattttgggt aaataaaata atataaaata
    22381 taaattttaa taaaaataat aaagtatata ttattgcttt aataagaatg taaaaaatat
    22441 ttttaaatta tcatttaatg taatttaaat gaattgaaga tatatgttta ttgtatgcaa
    22501 ttgttgtatt tttaggtttt ttgtgattaa ttaaaaaatt ataaaaatcg tttacatgtt
    22561 gatttttaaa aaaaaaagat atagaattat atagaaagta ataaacaaaa attggattta
    22621 cgggagtgga atgaaatgat gaatgttacc ttaacagatg taaaagaggc tagagagacc
    22681 attaagaaca tagtaaaaag aactgactta cttgaaagtg ttaaacttag cgaaaaaaca
    22741 ggagcaaatg tgttctataa atgtgagaat cttcaaaaaa caggttcttt taaattaaga
    22801 ggagcttgta acaaaatagc gagtttaaca gatgaagaaa aggcaaatgg agttatagct
    22861 tcaagtgcag ggaatcatgc tcaaggagtt gcattgggag caaaaatgac tggtataaaa
    22921 tctacaatag taatgccagc aactgcgcct ttagcaaaag ttagtgcaac taaaggttat
    22981 ggagcagaag tagttttaaa tggtgcggtt tatgatgatg catatgcaaa agctgttgaa
    23041 atacaaaaag aaacaggagc aactttctta catccattca atgataagta tgttatagca
    23101 ggacaaggaa ctatatctct tgaaattttc gaacaattag acaataaagt tgatactata
    23161 ctatgcccag ttggtggagg aggaatcatc tctggtgttg ctgttgcagc aaaagcatta
    23221 aatcctaatg taaaaatagt cggtgttcaa actgcaaata taccttcaat gaaagagtca
    23281 ataaaaaatg gaaaagtaac aacagcattt aatgatacta caatagctga tggtatagct
    23341 gttaaaactc caggagattt aacttttgaa ataataaatg aattggttga tgaaatagta
    23401 gtggttgaag agacagaaat tgcagaatca atattattta tgatggaaag tcaaaaaata
    23461 gtttcagaag gtgctggtgc tgtatgtaca gctgctatat taagtggtaa gtatgtacca
    23521 gcaaaagatg aaaatgtagt ttgtataata tctggtggta atatagacat aaatacgtta
    23581 tatagaataa taggtgttgc tttagctaaa gaaggaagaa gatattcatt tagtacaatt
    23641 atggaagata agccaggaaa ctttgctgag ttaactagaa taataagtga gaatggtgga
    23701 aacattttaa gtgctaatca aggtaaatta tcagcagggg aagctctagg aaaacaaagt
    23761 gcggaattta tattagagac aatagattat gaccatatag ctagaataaa gaaagctata
    23821 gaagaaaaag gttttaaaat tatagaatta taataaaaag tgtatataat atacaagaaa
    23881 aatagtataa tagtgtatga agatacattt tatgtgtatt agattaacga attaggagga
    23941 ttttaaaatg aaacatgaag taatacatac taatgatgca ccagcagctt taggaccata
    24001 ttctcaagct ataaaagctg gaaacttgtt atttgtatct ggacaagttc ctcttgtacc
    24061 agagactatg gaagtagttg aaggagatgt acaagctcaa actgctcaat ctttaaaaaa
    24121 tcttaaagct atattagctg aaagtggagc tgatttttca aatgtagtta agactacagt
    24181 atttataaaa gatatgaatg aatttggggc tataaacgaa gtttatgctg aatattttgg
    24241 agaaaataaa ccagcaagag cttgtgtaga agttgctaga ttaccaaaag acgttaaagt
    24301 tgaaatagaa gtaatagctg tattataaaa atacaatatt tttaatttga aaaggctgaa
    24361 aaactatatt ctagtgattc agccttttta taaaaaaata aatcacaata aagaacatga
    24421 taaatattgt cgaattaaaa aataaaatta aaataaatat tgcaatattt ttgagaaggt
    24481 tttataatga gtatataatt tgaaacgtgt aactattaaa ttaggcatga gttcttggat
    24541 atttttatcc tgaggattca tgcctttttt gtattgaaat tcaaaaatct atttaataag
    24601 gaggaagtac tgtgttaaat tttttcaaga aaaataaaag ctataagtta catgcagtag
    24661 tgagtggtaa cagcattaat attgagaaag taaacgattc tgtgttttca aaaaagttaa
    24721 tgggagatgg agttgcaatt attcctaata gtaatgttgt tgtagcccct tgtaatggta
    24781 aagtaactgt gcttacagaa tcaaagcatg cctttggtat ggtctcagat gagggtgttg
    24841 aaatattagt ccacatagga attgatacag ttagtttaca gggtgagggg tttaaaaatg
    24901 aagtgtctca aggagatact gttaaaaaag gtagtccaat aataagtttt gaaagagaaa
    24961 aaataaattc tcagggaatt gattgtacaa caataataat tgtattaaat catagcgaat
    25021 tttcagaaat aaattgcatg gtagaaaatg aagttgttgc agggcaagat actgttattg
    25081 aaattatgaa ataatagaaa gggattaatt caatatgaga gtagtaaaaa tctttaataa
    25141 taatgcatta tctacagtaa ctaatgatag gaaagaagct attttactag gtcttggtat
    25201 aggttttaat aaacgtccag gagataaagt taatgaagat aaaatagaaa agatttatta
    25261 tgttcaagac cacatgcaaa caaaattttt agaattatta aaaaatgtta gtccagaagt
    25321 gatggatgct tcacaacaaa ttataagtct tataccagga aatgagggaa aatttaataa
    25381 taaagggata ttatctttag tggaacatat tagttttgca atagatagaa tgaaaaataa
    25441 tgttttttta cctaatttaa tgcttttgga tataaagatg atgtattcaa aagagtttga
    25501 attaggagta aaagctttag aaattatata tagagtatgt catatccatt tgcctgaaga
    25561 tgaagcaggt tacatagctt tacattttgt caatttacaa tcaaatgata atttggcata
    25621 tgatacttta aaatttgtaa aaggtagtat tgatttgatt aaagaatgtt atggattaga
    25681 attagacgaa tcgagtttaa gtacattacg ttttagaact catcttaaat ttcttgctca
    25741 aagaattttt aagaatgaaa tttgtcagga tgataaaatg attgaaatgt atgattattt
    25801 aattaacaat catcctaaaa ataaagaata tttagaaaag ttaaatgtat atattgaaaa
    25861 ggagtttagg tataaattag ataaacctga aaaaatttat ctactactac acctaacaaa
    25921 aatcttataa gaatgagatg aattagtaat agaaatatat ataattttag ggggggataa
    25981 gattgaaaga aaaactactt ggaggactgg aaacatttgc aaaggcaatg gttcagccat
    26041 taatgtattt atcgagtgca ggaatattaa tggtggtagg tgtactactt acaaatgata
    26101 tgttattagg agtacttcca ttttttaaat gggagccaat tcaaatattt ggaagcctaa
    26161 tttataactg tataatggct attattaata atcttgctgt tatatttgct ataggtattc
    26221 ctgctgcact agcaaaaaaa gagaagcaca aagcagcgtt aataggattt atgagttata
    26281 taatatacct tacagcttcc aacactatgt taacatcact aggtcaacta gcaaaagaaa
    26341 gcaagatgct tggactgata gggactggac aagctactat tttaggtatt caagttgtag
    26401 atacaagtgt atttggtgga attattttag ggtatatagc tggatatgta tttaatcgta
    26461 caagcaacaa aacatttaaa ggagctttac agatatactc gggagcaaat tttagtttta
    26521 tatgcatgtt ctttgtatcc attttaattg gagttgtgag taactttata tggccattag
    26581 ctcaacaagg aatagcttct cttgcaaatg tcatcaaaga tacaggtaca tttggtttat
    26641 tcttatatgg ttttttagag agaatattag taccaactgg acttcatcat ttaatttata
    26701 caccatttca gttttctgat ttaggaggag tgcttaaagt tggtgcagat acagtttcag
    26761 gagcctatgc tatagttatg acagaaatga atttaccagt tgagcgtttc tcagatagta
    26821 tatactacat ggcaactgga tttacaaaga tgtttggtta tataggtata ggagctgcct
    26881 ttatttatac tgcatataag caaaataaga agaaggttaa ggcaacgatt attccactaa
    26941 taattacggc agtattagcc tctgtaacag aaccattaga ctttatgttt gtattctgtg
    27001 caccactttt attctttgta catgcagtta tagctggtct ttttatagct ttattaaaaa
    27061 tatttgatgt tactgcatta tgtggaggga acttattagt atcagttata atgaatacta
    27121 cagtaggcgt agaaaaaaca cactggccag taatgatgat attaggactt gcacaaatta
    27181 ttgtatattt tgtagtattt agttttttaa ttaagaaatt taattataaa actccaggtc
    27241 gtgaagatga gagtacagct acaggcgaag aacaatcaaa acaacttaat ttagatgcag
    27301 ggatagaaaa tataattgat gggttaggcg gaaaagaaaa tattaataca gtagaaaatt
    27361 gcatcactag attacgtgtt aatgtaaaag atgaaagtaa gattaatgaa gatataatta
    27421 atttaacgcc aaatagcggg attgtaagga aaggaaaaga tatacaaata atttttggac
    27481 ttcatgtgca tgaggtaaga cgtgcagtag aagatttttt agaagcttat taaagatata
    27541 tatttatata gcaagggtaa ataacagctt taagtgctgt ttactattta ttaaagatac
    27601 atataattta tagaggagga ataattatta tgattattac tattgttggt ggaggaagca
    27661 catttactcc aggtattgtt aagtcaattg cattaagaga aaaagagtta gagcttgagg
    27721 aaattcgttt atttgatatt gataaaaaac gtcaagataa agtagctgta gttgtcaact
    27781 ggatactaaa agaagatgtt aaaacagatg ttaaactaac tgtaactaat tgtgaaaaag
    27841 aagcgtatac agatgctaca tttatatttg cacaaatgcg tgttggtaaa tatgaaatgc
    27901 gtgagcagga tgaaaaaatt ccattgaaat atggttgtgt aggtcaagaa acttgtggtt
    27961 gtggagggat ggcatacgga ctgcgtacaa tacaaccaat gataaaggtt attgatgatg
    28021 tagagaaata tgctaaaaaa gattattgga tattaaatta ctctaatcct gctgcaatcg
    28081 tttctgaagc ttgtcgtaag ttaagaccaa atgcaaggat tataaatatc tgtgatatgc
    28141 caatagctat tatagatatg atagcggggt cattaaatat caatgacgtt cataatatac
    28201 gctatgatta ttttggtcta aatcattttg gctggtttac ttcaatagat tataaacata
    28261 gagacttaat ggaagagatt aaggaataca taaaagaaaa taaaatatta ttaccaaaat
    28321 cttatataga aaacttaaat aaaagtcgta gtacaggaaa tcgccatgct gtaagtagct
    28381 ggatacatgt ttgggaatct gtttatacaa tgttagaaag cttcccaaaa tatctgccaa
    28441 acacttattt aacatactat cttttaagta aagaaacagt ggaacattca aacataaatc
    28501 gtactcgtgc aaatgaggtt atggaaagcc gtgaaaaaga cttatttgaa ggtgtgagac
    28561 attacttaga aactggagaa atttctgaaa aagcatttta tgcaggttca catggagatt
    28621 ggattagtga tttggcagtt tcaattaaaa atgatacacg tagtcgtttt ttagttataa
    28681 ctgaaaatcg tggagctata ccaaacatgc catatgatgc aatggtagaa attccagcat
    28741 atattggtaa agatggtcca gaagttattg ctcgaggtcc aatacctcta tttcaacaag
    28801 gattaatgat gcaacaactt aacagtgaaa aattgatagt agaagcttat atagaaaata
    28861 gttatgaaaa agctttacaa gcattcacat taaataaaac tattccaagt atgaatgttg
    28921 ctaaagaaat tttggatgaa atgatagaag ttaataagga atactggcca gaattaagat
    28981 agtaggataa aagaaagggt ttttatttaa tcattagata tgttttcaga gtcttttatc
    29041 aaaatgtatt cacaaacata tggcaatata ttaaaataaa gagtgatatt tttagaagaa
    29101 tggggtgatg caatgaacat tataaataaa attttttggc aagaagatag gattggcatg
    29161 attacaaata atttagaggc agatacacat agccactgta tgttacaact gttcttaggg
    29221 atagaagaca gcattgaaat aacagttaat gaaaaattag ttaaatgtaa ttgtatcata
    29281 gtagataaaa atatctctca ttcattttca gcaagaaaaa aagtttatta ctctgctatt
    29341 attgaaccta catctatata tgcagaacag cttacctcaa aaatgaatga ttttggctac
    29401 tggatatgtg acaatgatgg attagagaaa ttacgtcaac aaggaacttt tttgatatct
    29461 aactctagta aagaacaata tttaagattt atggaaatgc ttaataacta tttgaatata
    29521 cctataactc tcaaacatta tgatgacaga attactgaat tgttcaattt attacatact
    29581 tgtaattgtg ataatcatac tatttcaagt tttgcagata aagtgtctct atcagcaagt
    29641 agattatcac atctgtttaa agaacaaata gggatacctt taaaaagcta tatcttattt
    29701 catcaaatgg agtgtgcttt tagagaatta ctttctggaa aaaatgttac cgaagcttct
    29761 atgactgctg gattcgatac tccatcacac tttgcaggga cagttaagcg aatgatggga
    29821 atgcctgttt ctttatcttt gaaagatagc gagtttttga aagtatatta aatattctcg
    29881 ttatataatg aagttgtact aagaaagggg ctttgttatg aataaagaat atttagaaga
    29941 aacaaaaatg ttaaattata atgaaccaca acttaagctt ttggtatcat caaagaattg
    30001 gttggaatta gatgattttc ataaaataaa atctatttat gagtttgtac agaatgatat
    30061 tttatttgga tataatgctt ctgatatgtt aagcgcaaca caggtattaa atgatggtat
    30121 gggacaatgc aatacaaaag ctactctttt aatggcatta ttacgtgcag tgaatatccc
    30181 ttgtaggctt catgcttttg atgtaacaaa agattttcaa cgaggagcta catccaaatt
    30241 gatttcacta ttagcaccaa aatatattct tcatacttgg gtagaggtat tttatcaaga
    30301 taggtggatt gctctggagg gtgtcataac tgataaaaaa tatttagaag caattcagaa
    30361 gaaatttttt aatcatggtg gaacgtttaa aaagtatgca atagctacaa atgatttgaa
    30421 aaatacatct attgattggg atggtaaaga tacttttatc caaaaagaag ctatcgtgta
    30481 tgattatggt atttttccat caccagacgt atttttttct actcattcac aacatatgtc
    30541 aaaattaaaa aatttcatat atgtacatct tatcagaaaa ataatgacaa aaaatgtatg
    30601 taaggctaga aacaattata ttgataaaaa tgaatgagtg tttaagaggt aactatatag
    30661 gaaatatata gaataattaa aactgtactg gttaaaatga ataaaaagga gttatcacag
    30721 agcaaactta aattgttcat aggataactc cttttctgta ttaacttcta ataatttgat
    30781 gtctttttat aaaacatata gatacaatta aaccagtaaa tgcaagagta gctgctattg
    30841 aaaatgcaac atctattcca tgaatgttag acagcatgtt tgaactcata tgagctttct
    30901 tagtagcact tgtcattata gtaataagta tagcagaacc tatagctcca gatatctgac
    30961 ggagtgtgtt attaattgct gtggcatgag atataagttc tctatctaat gttttaattc
    31021 cccatgtagt taatggcatt aaaaccattg aaataccaat catacgaata caatacatca
    31081 atgacacatg aattaaagat gtatctctgc ctaaaaaagc gaaggataaa gttcctaaaa
    31141 acaaacatcc tgttcctaat attgatagag catgaggtcc atatttatct aaatgacgtc
    31201 ctgcaattgg attgagtaca accatcaata tagcaccagg taacattaat gaacctgaag
    31261 ataatgcaga aaatcctctt acagattgaa cgtacagagg aatcattaat gtagcagaca
    31321 tcattgaggc atatacaatc ataattaata ttgtagaaat aagaaatggt ttatttttaa
    31381 aaaccctcaa ttctaaaaaa ggttctttag attttaattg acgcaatgta aataaaatta
    31441 atgacataat tcctattaat attggtaaat atgtggcaat attagtccac ccataattac
    31501 cttgattagt aaatccaatt aataaaccac caaaacctag agatgatagt actattgaag
    31561 gtatatctaa ttttagtttt tgagtttccc caacatttct taataaaata aatgatagta
    31621 taacatctaa tatagcaata ggggaaagaa acagaaataa actatgccaa ccaaatgaat
    31681 ctactaacca tcctgataaa gttggaccta ttgctggagc aaatcctact gtaataccaa
    31741 ctaaactcat agctgcacca cgtttttcct cagggcataa atagagtata ataacttgta
    31801 aaagtggcat tagagaacca gaacccattg cctgcaacac acgagcaatc aacatagttg
    31861 aaaaattatt agaaaaaagt gcaataatac atcctataga aaagatagac attgatgtaa
    31921 taaataactt tctagtacta aatcgattta ttaaataagc tgttgtgggt acaataatac
    31981 ctgcaataag tagataaatt gttgttaacc actgaccaac atcagcagta attttaaaat
    32041 ctgacattat attaggtaat gctgttgaca atagtgtttg actgagagaa cttataaaag
    32101 caccagtcat tacaattgct attattaaat ttcctttttt attgttactt tgggtttcca
    32161 taatttataa cgtctccttt tttaattgat ttttaatgct gttcaataaa agtacttttg
    32221 gtcttaaagt gtcttctttt tctaaatatg caatcattgc atccataatt aaagaatcat
    32281 attgttgatt ttttccctcg acgtataatt tttttattgc ttctagtaat ttaggatttt
    32341 caaggagaga agtatcagtt ttgattagtt cttctatatc ttgtatcatt ttaaaaaata
    32401 gcgatttatt atgaagggaa aagtaaactt catcttcttg tttatgtaaa aacattgatt
    32461 tttcaattaa aggatttaga ttatccatat tttctacaac taaatctaga atttgtatag
    32521 taaatttggc aataaagtat acatttattt ctcttttttg tacatgaata atgtatataa
    32581 actgatgaat tagagcatcc aataagaaaa ctaaatcaaa tataattttt tctattttat
    32641 caccatagtt atcaaataag cttaatttgt attcattcaa taagttagct cgtgattttg
    32701 tttgcaattt acttgtagcc ttacgttgct cttcagaaaa attttctctt atataggtat
    32761 ttatggtagt tctagaaaaa atatttttcc atacagcaat tatctttctg tttaactttt
    32821 ctgatgctgg aatgtcatta ttttcattta tggaatgtat aatttctaaa aacttttcat
    32881 cataataatt gattatttcg cagataaaac tttccttagt ttcaaaatgc ttgtaaaaag
    32941 tagctttaga cattttacac tcttttacaa catccgatat tgaagtattt tgaaaaccct
    33001 tttggtcaaa aattttttgt gcaatagtta taatgagcct ttgtttttct gtcactttat
    33061 tatctccttt cacgcaaaaa cgtttagtac tatagagtac ctacaagttt taataaatat
    33121 attatacatc atttggaaaa aaaatcaata tatagactgt aatatgaaat ttttgaaaaa
    33181 tttgttgtta tttttagtcg tataaaaaag aaacttaaga gtttcttaag aattgtttaa
    33241 agaagttgca ataaattagt ggtatgatta tagtatgaaa gagaatgtct tgaaattttt
    33301 ctgtatcaaa ttccaattat ggaggtgtag tatatgaaaa agaaaatttg tgtagcaata
    33361 atttttgctc tatttatagt ttttgtagaa atgtttattg atagctctag cgaaggaagt
    33421 aaaattatga cagctgaatt aagtaaatac aggaaaggcg taaatgtatt atcaataaac
    33481 aatattatag tagcgaataa aaaatatagt ttgcccaaag attattctcc tcaggaaagc
    33541 agtgaagcga gagatgcttt ttataaaatg aataaagatg ctcaaaagtc aggattaaat
    33601 ttaaaagctt ttagtacata tagaagttat gaatatcaag atagattatt taagtcatat
    33661 gtaaaagaac atggagaaaa agaagcaaat agattttcag ctaaacctgg agagagtgag
    33721 catcaaactg gacttgcttt tgatattgga ggagatgacc agtcttgttg ggcaaataaa
    33781 aagttcaaca atacaaaaga agctaagtgg ttgtatgaaa atgcatataa atatggtttt
    33841 atacttagat atccagaagg taaagagaat ataacaggat atatgtatga atcttggcac
    33901 tatagatatg ttggaactga acatagcaaa aactttgcaa tgaataattt gactttagaa
    33961 gaatatttac atataaatta agagatggtt tgttaaaatt aggttaaaat aaaaaataac
    34021 ctagactatt agctgtgtaa agtgatataa tgtaaataca atttagataa ctactcgaag
    34081 gggagtagct tacacgaaag tggtaattaa tcgtcaagac agtagaaata ctcggttaat
    34141 tagcaaggga ttccttgata gcaagacctt caaaataagc attaggtatg tttgttttgg
    34201 ggtctttttt tattcaagga atttttattt aaaaaaattt aaatttcaag tatacataag
    34261 agcccccaaa cataaaataa atttattgaa gaggagggca acaaatgtct tacaaaagta
    34321 cttatcaaga agtaattaaa aatttagact caagtttatc tggtttgagt aaagaaagag
    34381 cagaagagtt gttagaaaaa aatggagcta atgaattaaa agaagcagat aaggttccta
    34441 cttataagct tttcctagaa agttttaaag acccattagt aataattcta ttgatagctg
    34501 ctttagtaca aatattttta ggtgaaacag tggagtctat aataattttt gcagttatta
    34561 ttataaactc tgttctgggt gttgtacaaa caaaaaaagc agaaagttca ttagaaagtt
    34621 tgaaaaattt atctgcacca aacgcaaagg taataagaga caataaaaaa atgacaattc
    34681 cagcaaaaaa tctagtagtt ggagatattg tattcttaga agctggcgat tacataccag
    34741 cagatggaag gcttatagaa gcacaatctc tcaaggttgt agaagggatg ttaactggag
    34801 aatcagaacc agttttaaag catacagaaa aaataagtga agatgtaagt ttaggtgacc
    34861 agaaaaatat ggtatttagt ggtgctgtag ttgtttatgg tagaggttct tttgtagtga
    34921 ctggaacagg tatggatact gaaatgggta aaatagctgg gcttttagaa agtgctgaaa
    34981 ataaacttac accattgcaa caaaaaatag aggattttag taaaaaactt ggtgtaggta
    35041 ttgtaatact tgctctttta atatttataa ttcaagtagc aagaaattac tttatggctg
    35101 gaaatggaga aatgacacaa attattttag attcattcat gttctctgta gcagtagcag
    35161 tagcagctat accagaagct ctatcatcaa tagtcactat agtattggct gttggaacaa
    35221 atagtatggc aaaaaaacaa gccataataa gaaaattacc agcagtagaa actttaggtt
    35281 caacaagtgt aatatgtact gataaaacag gtactcttac acaaaataaa atgacagtag
    35341 ttgattatta tatgtatgga actaataatg atgagttaga taaagaaaaa gtaaactatt
    35401 cttatcatgc taaattattg actatggttt ctacattgtg taatgactct catataacta
    35461 gtgatggtaa agaagttggt gacccaacag aagtagcttt aataaattat tcaagtaaaa
    35521 atgatttaga ttatgataaa ttaagaagta agtatatcag aataaatgaa ataccatttg
    35581 actcagacag aaagcttatg tctactgtaa atagtatata tggagattat ataatgttta
    35641 caaaaggtgc tccagatatt gtgtttagta gatgtaaata tgctttgaaa aatggttcaa
    35701 aagtagatat aacagatgag ataatagaag aatataaaca taaaaatgaa gagttttcaa
    35761 atagagcact tagagtatta gcttttgcaa taaaagatgt cccagaagaa gattttatac
    35821 cttcaattga tgacgaacat gacatgacat tggttggaat aatggcaatg atagacccac
    35881 caagagaaga agtaatggat gcagtaaaag aggcaaagag tgctggtata aaaactgtta
    35941 tgataactgg tgaccataaa acaacagcag cagcaattgc aaaagagatt ggtataatgg
    36001 aagaaggaga cttggctctt actggaaaag agcttgatgc tttgagtgaa gaacaactat
    36061 atgaaaaact agaaaatata agtgtatatg ccagagtctc gccagaaaat aaaattcgta
    36121 tagtaaaagc atggcaacat aagaataata taacagcaat gactggtgat ggtgttaatg
    36181 atgcacctgc attaaaacaa gcagatatag gaatcggtat gggaagtggt actgatgtag
    36241 caaaagatgc ttctgctatg gtattggttg atgataactt tgcaagtata gtaaatgctg
    36301 ttgaagttgg tagaacagta tacagtaata taataaaatc aattacttat ctatttgcag
    36361 gaaacttagg agctatagta tccatagtat ttgcagtgtt tgcaggttgg tcaaatccat
    36421 ttacagcttt acaacttttg tttataaact tagtaaatga ctctttacca gcaattgcat
    36481 tagggtttga aaagtcagaa aaaaatataa tgaataaacc tcctagacac ccggatgaaa
    36541 gtatactagc aggaggaaca ttgaaggcag ttgctataag aggttgtata ataggtgtag
    36601 ttacaataat tgcacaatat ataggattac aagtatctcc agaacttgga gtagcaatgg
    36661 cattttcaac attgatatta gctagaataa tgcaaacatt acctgcacgt tctaataatc
    36721 aaacagtaat aaaattaggt tttttcagca ataaatacgt tataggagca gttttagtct
    36781 gtcttgggct ttactcttta acactaatac cagtgctaag aggaatattc tctataccaa
    36841 caacgtttgg attgtaccag ttggggattt gcctagggtt agcattaatt tcatcaatat
    36901 taatggaact taacaaatta ataaatagaa gtgaagtaaa ataatcaaaa aattaaaggg
    36961 catattgatt ttaacaatat gccttttata aatagtctaa tttattctat attatcgatt
    37021 atttggaatg cttttataac ttcaggtaat tctttttctt caggcattct aaatcttatg
    37081 aaatttttat caagtccagt aaaatcactt ccagatataa ctcttatttt taatttagca
    37141 aactcttgtt gtaaatctat gtccttgttt ttatgttcaa ctgtcataat tgatacagta
    37201 tcactagttt cagctatatt aaggtttttc catggaatta ataattgatt ttttatatct
    37261 tttgtttttt cttttaattc ttctataaat tgaacatcat ctaataaatt tgctgcaata
    37321 ctacgagata tttcagacac ttcgtaagga gtagaaatct ttttaatagg gctaacaagt
    37381 tgttctggaa gaactgcata tcctgctctt agtcctgcta aaccgaatcc ttttgaaaat
    37441 gtttttaaag caattacatt atcataatta tttagaagct tgacagctga attttctttt
    37501 ggcatgtatt caccataagc ttcatctacc ataactgcta tatcatattt agctgcttcc
    37561 tttactatat tttcaattga tgataaatgt ataatttgtc ctgtaggatt gtttggattg
    37621 tctatgtaaa ttactttata ttctggattt attttttcaa taaattcttt ttcattaaac
    37681 ttgaaattat cctctttctt taaaagaaca taatcataag ttgcaccatg catttttata
    37741 tctgtctcat attctgagaa ctgaggtgaa tatcctaaaa ctttatctcc cttttcaata
    37801 aatagtctat ttaaaaggta aataacatgt atagaaccat ctgccaaaca aattctgtca
    37861 gtatctaatg ctataaaatc tttccaataa tcgactatat tatcttttaa agcaattgaa
    37921 tgaggatatc ctcttatcat atcaaatttt aaagcattaa aagcttctac agctttgttt
    37981 gagtaagaca cggtatttat accttcacca cagtctatct cgaatacatc ttctgaatgt
    38041 ccagattcta ttgcataact ttttttcaac tgcatttcaa tagctttgtt aatttttagt
    38101 ttcatacttt atcactcctt atttagtcaa taataaatgt tataaaagtt gctagattta
    38161 aaatattatg aaaatatata atagtaaaaa atgaatataa atattataat agctcacttt
    38221 atttaatatg attatatatc atatattgta aaatttctag cattataaga ctataaattg
    38281 aaaatattca gaaaaatact acaagaaacg atttattttg ctcttaaaat aagttaaatg
    38341 aatgttatta attttgtaaa ttgaaatagt ttaagtataa agttatgata taatctaata
    38401 agggtaattc tttagattat attaaggaaa tataggtata caataaaaag ttatggagtt
    38461 ggattatggg atataaatta attgtaacag atatggatgg aactttatta ggcaataatc
    38521 ataaagttac agatgaaaat aaaactgcac tacaaaaagt tataaaaagc ggtattaatg
    38581 taactttagc aacaggaaga gccttcgatt cagctaaatg caatgtagat tttcttaaag
    38641 aggacatgcc aataatagct tgtaatggtt cattaataag agagcaaaat ggaaatataa
    38701 tatattcaaa taagattgat acaagaacat gtttaaacat tttagatgta ttagataaat
    38761 acgatattta ctaccaatgt aatagtatag attctatgct ttcgaaaaaa atagaaggac
    38821 gtgaagatag attaagtgta tttttaggct ctgaaacaga agtaattgta aaagatgatt
    38881 tgagagaaga aatctttaaa aaagatatac taaaatttgt aatcatagaa gaaaaaaatc
    38941 catctatttt agatgaaata agaaaagaat tgagaaaagt tcaaggaata aagataactt
    39001 cttcatggcc aaacaatatt gaagtcatga atgaaggtgt tgataaaggt aatgcagtta
    39061 aaattttagc tgaaaaaatg aatatagaca gagaagatat aattgcattt ggagacaatt
    39121 acaatgacat agaaatgata aagtttgctg gtcttggggt agctatggga aatgcagaag
    39181 aattgattaa acaagaagct gattatgtta cagatacaaa tcaagacagt ggagttgcaa
    39241 aggcaattta taaattccta gagttaaaag aatcacttag ctcttaaaac agtatataat
    39301 aggaaaaaat aggttgacta ttttgtagat agtaattata atacaaatag ataaaacata
    39361 aaaatttaga ttcagtgaaa aagagagtag atatagagaa aatctcagcg aattggggga
    39421 tggtgtgagc ctaatgataa cactttatat tgaaaagaac tttggagaat actacttgaa
    39481 attcagtatg gtagtaggta atctcgcctt aagagaatag agtgggtaaa cattgtttac
    39541 caactagagt ggtaacacgg aatatttcgt ctcttggtct ttttgaccaa gagatttttt
    39601 agtttcattg agttacaaga cttataatta aaatacaaaa aattaaaata tgaggtgaaa
    39661 gttatgaaat tatggggcgg acgctttaga aaagcggaaa atcaacttat ggaagaattt
    39721 aataaatctt ttggctatga ttgtgtactg tacaaaaaag atatcgaagg aagtgtagca
    39781 catgttcata tgcaagtaaa atgtggactt ttaacagaag aagaaggaaa gtctataaca
    39841 gaaggtttaa aagggatact tgaagatgta gaaaatggaa agttagtatt ggatggagag
    39901 tatgaagata tacatagttt tactgagata aatttaattc aaagaattgg agatgttggc
    39961 aagaagcttc atacagcacg tagtagaaat gaccaagttg cagttgatat gagattgtat
    40021 gctaaagaaa aagcaaatga tttggtgggt ttaatatctg aatttaaagc tactataaaa
    40081 gatgttgctg acaaaaaccc agtgatgatg ccaggctata ctcatcttca aagagctcaa
    40141 gtagtgacat ttaagcatca cttaatggca tattatagta tgtttgatag agatgagaaa
    40201 agaataaaaa atgctataga aatattagat gaatcacctt taggctgtgg agctttagca
    40261 ggaactacgc atgatataga tagaagtata acttgtgaac aattaggatt taaaaaagta
    40321 gtagataatt ttatggatgg agtaagtgat agagattatt tactagagct aatgagtgat
    40381 ttttcaatta taatgatgca cttaagtaga ttaagtgaag agttgatact ttggagttct
    40441 caagaatttg gatttgttga gatagatgat ttatatacaa ctggaagtag tataatgcct
    40501 cagaagaaaa atccagatgg tgcagagctt ataagaggta agactggcag agtatatgga
    40561 aacttatttg gtctttttac agtaatgaaa ggaataccat tagcatataa taaagacatg
    40621 caagaagata aagaaggttt tttcgatagt gtacatactc ttgaaatgtg tatacaaata
    40681 atggatagaa tgatagccac actaaaggta aatgaggata aaatgaaaca agcagtaaaa
    40741 aatggttttt taaatgcaac agaagttgcg gattatttgg ttaaaaataa tgttgctttt
    40801 agagatgcac atggtatagt aggaagtatc gttatatatt gtgaagataa taaaaaagca
    40861 atagaagatt taacattaga agaattacat aagttttcag atgcttttaa ggaagatatt
    40921 tatgatttta tcgactatga gagtatatta aataagggaa taaaaaagaa tttaaaataa
    40981 tgtatttatt tttaaaagct gtaaatttga atgttatcat ttaaatttac agctttttat
    41041 tgtgtaagat tttattatat aaaagtaaga aacttaaaag tagtagtatt taaaataaat
    41101 acaaataaaa aattgttatt aaaatgataa ttaatatcaa taagtattga caaaattatt
    41161 tctaaatact attatatata atataaataa taatgaaaat caatatcaaa taaatgggag
    41221 gaaatctaat gaacttatta gtgattggtg gtcatgaaag aatggaaaaa gattatatgg
    41281 ttatggccaa gaaaaaaggt tataagacaa aggtatatac taccatgtca tctaaattaa
    41341 ataattctat aggaagacca gatgcagttg tcatacttac atcaactgtt tcacataaga
    41401 tgtctagaac ggttgagtct caagctaaaa aacaagatat attaatcgta aggcataaaa
    41461 atagtagtaa ggtagcattt aatgaatgtc tagatatggt tgatgaatgt ctgggaaatt
    41521 gttcaaaatg taagcttaaa gacaaacaaa aacaaaaaaa tatataggta taaaaaatac
    41581 ataaacataa aattaaaagt actgagataa tagttataat tattatctca gtacttttag
    41641 atttataagg ataagtatat tataaatttt ttattataat ttccaactac ttgttacttt
    41701 ttcatatttt ttttacttct agcttttttt ctttttttct tctttatatt tgaagctatt
    41761 ataaataaaa ctaaaagcac tagaatacct gcaataatct taacaaaagt cattaaaata
    41821 ttatttccag taaaaaatgg aagactgcta tcaagattat tttttgcaat taagtctata
    41881 ctgttttcta gtttaccatt attatataca tttaaagtac caaccttagc cccttttttt
    41941 ataggcaaat caattttatt taactctgtt ttggtatcat aattgccttg agattcatct
    42001 ttttctaaga atatcctata actatttttt ggttcataaa tcaattcatg ttgttttgta
    42061 aacaatactt ttttagtatc tgtgtatttt tctttatcaa caatagtagc acacttaaag
    42121 ttatcaaatc cataatcaat cagaatacgt gagtctaaat ataaatcagc attagttgaa
    42181 ttaaatacag caacgataac tcttctgcca tcttttacag cactcgataa aagacattta
    42241 ccagcagcat cagtatatcc agtttttata ccatcaacta tatcatattt tatatttata
    42301 ggttgacctt tgtatgtaat cttatcatgt gatgtcaaaa acttattagt gtttacaaag
    42361 tatctctcat aaggatatgc ttttgtagct tcaaatttaa gtgacttagt ttttacaatt
    42421 tgtctaaaag tatcattatt catagcttct ctagctatga gagccatatc atgagctgtt
    42481 gtgtagtgat ttgggtctgg taatccatga ggattattaa aatgagtatt agtagctcca
    42541 attttttttg cctcagagtt cataaggtca ataaattttt gtacattgcc accaccaaca
    42601 tatctagcta atacaaaagc agcatcatta gcagaatgaa ccaaaagagc atctagtaac
    42661 tgctttacag taaaagattc cccatttttt agatacatac tagagccatc tatttgaggt
    42721 aaatctttaa tttctattac ttgatttaaa tctttaacct cctttagaac taaacaagct
    42781 gtccatactt tagttgttga agctggataa agcttagaat ttccatcctt attatatagt
    42841 attttgccag actcatagtc cattaaaact gcatgtttag caactatagc tggttcatta
    42901 tctgcaaaag agatattaga gcttcctaaa aatgaagtaa atatcaataa acagattaat
    42961 aaaagtgata aatttctttt cattgtgagc ctccaaattt taaattcatc ttttagtata
    43021 gcatatattt gtcaataatt aaaagaaagg agtgattaat atatgaaaaa aaaagtaaaa
    43081 tttatttcgt tttttgtttt atttatttta ataatatcat caataatcgt ttataaatcg
    43141 cttattttca aagatgatat ttatgttgta agtaaaaaaa atttacctaa tgaaaaaaca
    43201 catgttgtag agaatgaaat taaaggtgaa gataataatc acaataaagt aactaaagac
    43261 atagataaaa attttcaaaa taataaaata actatataca taagtggagc agtaaataga
    43321 cctggaattg taactataga gtcagacaag agactttatg atgctgtaga attactagga
    43381 ggaacgacaa aagaagcaga tttaaatgga gtcaatctat cagtaagatt agaagatgaa
    43441 caacattaca taatacccaa aataggtgaa gcaacatctg ttacaagtaa tgatgattcc
    43501 aataaaccaa atcagaggaa tgagtcaaga ggtgagccta aaaataaatc caataaaaat
    43561 atatctaacg aatcaaaagt aaatataaat gtagcgacta tagaagagtt ggattctcta
    43621 cctggggttg gagaagctac agccaataaa attttacaac acagagaaga aaatggacag
    43681 ttcagttcaa ttgaggaaat aaaaaatgta aatggtattg gagataaaaa gtatgaaaat
    43741 ataaaagatt taatttgtgt agactaaaat ataatacaaa acttacatat agatatgtta
    43801 ttataaatat gtatgtatat gtgtgtacat gtggaaattt agtaaaggag gcacttttat
    43861 gaaaaaagaa caaaaaaaac ttacagagat gacaacagca ggtggttgag ccgctaaaat
    43921 agggccagag gttctggcat cagttttatc tcaattgcct aaaaatgata atatagaaaa
    43981 tttattagta ggactagata cagcagatga tgctgcagtt tataaattaa atgatgatat
    44041 ggcacttatc caaactttag acttctttac accaatggta gatgacccat acgtatttgg
    44101 tcaaattgct gcatcaaatt cactatcaga tgtatatgct atgggaggca aacctttggt
    44161 tgctatgaat attgtttgtt ttccatcatg ccatgatatg gatgtacttg cagaaatatt
    44221 aaaaggtggg tttgataaag tgaaagaaag tggagcactt ttggttggag ggcatactgt
    44281 agatgacaaa gagcctaaat atggtctttc agtttctggg attgtaagtc caaataaagt
    44341 tttatcaaat gctactgcaa aacctggtga taagttaatt ataacaaaac caattggagt
    44401 gggtgtatta aatacagcca tgaaagaagg tatggtagaa caacatatag cagataaagt
    44461 aatagaaata atgatacatt taaataaata tgcagcaatg agttttgaga aatttgatgt
    44521 aaattcggtt acagatataa ctggttttgg tctacttggt catactttag aaatggccaa
    44581 agcttctgaa gtaagtattg aaattgaatc aaaacatgta ccaattattg aaggtgcaat
    44641 agaaatggca caaatgggaa ttatacctgc tggtatgtat aaaaatatgc attatgtatc
    44701 taaagatgta gaagttgtag gaaatataga agttgcagtt caagatattt tatacgaccc
    44761 acaaacatca ggagggttgt taatttcagt aaaagaagaa cttgcagagg aacttgtaaa
    44821 agatatgaaa ttaaatggag caatagaagc taaaattata ggttctgtag ttccaaaagg
    44881 ggaaaaatat attacagttt tataaaatat tatagagaaa gagtgattgt tttgagcaaa
    44941 agagaattgt ttgcaatgtt gccttcagtt gatgaagtat taagtgataa tagaattgta
    45001 gaaataataa atgaatatcc tagaagttta gttttagaat ctgtgagaga agtaatagat
    45061 ttaaaaagac aatttatatt aagattaaaa gaagatgcat caaactctgt aacaatagag
    45121 tttggtgaaa taatagaatc tgcaatagag agagttaagt taaattactc attatcttta
    45181 aaaaaagtta taaatgcaac tggtacagtt attcatacaa atttaggaag gtctttactt
    45241 agtgaagata taaaagatga attatggtgt gctgcttcaa ggtattcaaa cttagaatac
    45301 gatttggata atggagaaag aggttcaaga tactctcatt taactagtac aataaaaaga
    45361 cttactggag cagaagatgt actagttgta aataataatg cagcggcagt acttcttgta
    45421 cttagcacaa tggctaaagg aggagaagca attgtatctc gtggagaatt agttgaagta
    45481 ggtggttcat ttaggattcc aagtataatg gctcttagtg gtgcagaatt agttgaagtt
    45541 ggttcaacta ataaaactca tttgaaagat tacaaggagg ccattacaga agatacaaat
    45601 gtattgatga aggttcatac aagtaattat agaataatgg gattcacaga gagtgtttct
    45661 atagaagaat tggttaattt aggtaaaaaa tataaactac ctgtaataga agacttagga
    45721 agtggagttt ttatagattt atcaaaatat ggtttatctt atgaaccaac tgtattggat
    45781 tctattcgac aaggtgcaga tgtagtaact tttagtggag ataagatgct tggaggtccg
    45841 caagcaggta ttatagttgg gaaaaaagag tatatagaaa aaatgaagaa aaatcaactt
    45901 actagagcac ttagagttga taagcttaca atatgtgctc ttgaagcaac attgagaatg
    45961 tatttagatg agacaaaagc tatagaaaat atacctactt taaaaatgtt gacatataaa
    46021 attgaagaat tagaagtaaa agcaaataaa ctatttgaaa aaataactgc tttaaactta
    46081 aatgcaaaca taaatataga agatggattt tcacaggtgg gaggagggtc tatgcctctt
    46141 gaaactataa gtacaaaggt aatatcaata actcctgaac atatgaatgt ttcatcttta
    46201 gaaaaaaaac taagacttag tgaagcacat ataatagcca gagtgtatga taataaatat
    46261 gtactagatg taagaactat atttgatgat gaatttgatg taatagttga ggaattgaga
    46321 aaagcattta attaaataaa tttgagttaa taaatattaa atttagaagt ataagtatga
    46381 agttttgatt aaaaaatagc tacacacact tgtgacttta gtcatagcag gtttagaggg
    46441 ggcaaaaaat gaaaaatgta gtaattggaa cagcaggtca tattgaccac ggaaaaacta
    46501 ctcttataaa agcattgact ggaagagaga ctgatacgtt agatgaagaa aaaaagagag
    46561 gtatttcaat aaacttagga tttacttatt ttgatttacc aaacaataga agagctggaa
    46621 ttgttgatgt cccaggacac gaaaaattta taaaaaacat gttagctgga gcatcaggaa
    46681 ttgatatagt tttacttgtt gtagcagcag atgaaggggt tatgccacag acagttgagc
    46741 atttagatat tctaagcttt ttaaatatca aaaatggaat agtggttcta actaagtctg
    46801 atactgtaga tgatgaattt agagagctag taaaagaaga tatttgtgaa aaaataaaag
    46861 gaacttttct tgaagatgct gaaataattg aagttgattc tatatctaaa agaggtatag
    46921 acactcttat tgaaaaaata gataaaatga gtgatgatat agaagataaa aatgaaaact
    46981 ctccagcaag acttaatata gacagagtat tttcaataaa agggtttggt actgtaataa
    47041 caggcacttt aatagaagga aagataagta tagaagatga tttggttata tatccacagg
    47101 tattaaaaac aaaaataaga agtatacaag ttcatggaga aaataaagat acagcttatg
    47161 caggtcaaag aacagcaatc aatatatcaa atgtaaaagt ggatgatata aaaagaggag
    47221 atgttttagc tgcaccaaac tcattggagg aatcaatgat gttagatgta aaattatcat
    47281 tggtaaatca tttagataaa ggattaaagc actgggatag attaagattg tatcatggaa
    47341 ctagagagat actttgtaga gcagttccac tagataaaga attaattgaa agtggagaaa
    47401 gtggctatgt tcagttgaga cttgaagaaa gcatagtatc taagaaaggt gatacgtttg
    47461 tagtgagacg ttattctcca atggaaacta taggtggagg tgtaataatt gacccatctc
    47521 ctaaaaaaca taagaaattt gatgaaaaag ttatagaagc attaaaaata aaagaaaaag
    47581 gcgaactaaa agatataata gaagagtact taaaaaggaa tttaaaaaat tatccaaata
    47641 taaaagaaat aatgagctat agtggtgctc atgaagaaga tgtaaaaaga gcgcttgaaa
    47701 cacttatatc agaagataaa gtatttatta taggaaatat gtatatgcat ataaatcaat
    47761 acaataaatt aaaagaaaat acaataaagc ttttaagtga gtaccataag aagtataggc
    47821 ttagaaaagg gatactaaaa gaagaagtaa ggtctaagat agagagtaat tttaagacta
    47881 gggagatgga tattttatta gaaaaattgt ctacagaaaa tgctataaaa atagaaaata
    47941 atatagtatc tctattagat tttgaggtta ttttaaatga taagcaaaaa gaaatagcaa
    48001 aaaaaataga aaaaagatta aaatcatgtg gagtttcttc tatactgaca atagatgaag
    48061 tttctgaagg aaatcataac tatgctgaag tattggagtc aatgataggt aataaagtag
    48121 agaaattaga tgatttgtat ataatggaca aagatattta tgaaaatgca aagaacattt
    48181 tgataaatta tataaaagaa aataaagaaa taacattagg agaatacaga gatttgattg
    48241 attctagcag aaagaattgc atgattatat tggaaaactt cgatagaaac aaaataacta
    48301 aaagagtaga aaacaaaaga attttgtttt agcagaaaat gtctgttttt gctttaaggt
    48361 ataaagaagg aattataatg tcgagtatcg tataattaat tagtataaaa tattaattaa
    48421 gaggaagtgt aaagtattga agaatataat acctgatgta aaaccagata taaaagaaag
    48481 agaagacatg agccttttat ttataataat gggctcagta cttttattat ttattataaa
    48541 agatatagag aaatatgaaa ctttattaca tgccatatat acgatatttt tagtagctac
    48601 aggagtaatt atatttaata ctacaaaatt taaagtagat tcattttcta catttttagg
    48661 attaattttt atagctactg gagtattgga gtgtatatat ttatttaatt ccttggggat
    48721 taaaactaag tctataatgg agattaacat cacaatatca gcaataacag atttatttcc
    48781 tatactgggt gtatacttaa gttttaaatt tgttaaggat aacaaacaaa tttattcaag
    48841 tgtaatttta tttatagcaa ctgctacatt aacaataaca tctttattca taatatcagg
    48901 aatttgtgac tatctgggaa atggtaaatc acaatatgct ttaggagtta taatatctat
    48961 attcataata attatttcta tcattagtgg aattgaatta aattcaagtt caaataaagg
    49021 taaatgggaa tatgatgaaa aaaagatttt taacagaata atagtaataa taatcttatc
    49081 aagagttcca aatctattgc atatagttat tgataataga aatgtagaga ggatattatc
    49141 acaaataata ataaatgtag ccttatatta tttatataat tacatagtat ctaagaatat
    49201 taagaaaaca gcactggaat tgcatgatac gaatgaagaa ttaacaaaaa aaacggaaag
    49261 tttaaaagaa aagaataaaa aattaatcta tgagacagaa aagatagagg aacttaaggg
    49321 aatacttatg aaaagagaat ctaggctaca atctacatta gatgtagcag taaatagcat
    49381 agtagtattt agcaaagatg gagaaattac atatgcaaat aagagcttta gaaatacttt
    49441 tggagactat aaagaacaag ataaattgac tgataaaata aaaaacttca atgatttgat
    49501 agaaagcatt cattatgtgt ttgttcatga gaagaatgca gagaaactta ttagtacaag
    49561 cgataataaa gtttatcaag ctatatttgc acctttaatt attgcttcac aaacagaagg
    49621 agtattgtgt gtactagaag ataagactaa gaaaaaggag tacgaacgca aacttattga
    49681 agcaaataag agaagtaaag attttttaga aagtattgga gatggcatag tagtgcttga
    49741 aggtaataac aaaatatatg ttaataatgc atgtagggaa atttttaaaa ataatttaga
    49801 gagtattgat ttttcattag tatgtaaaag tgaagaaagt atagaaaaaa gatatgtaat
    49861 agatggtgaa gtaaagtatg tagagatgag tttttctcag tatactaatg ctggtactaa
    49921 taaaactatt attgtaatac gtgatacaac ctctcgaaaa atatctcaaa taaaacttga
    49981 ggaaagtcaa tcatcatatt caagatttat agatatatta cctgatggca tatgcttatt
    50041 aaaagaagat ttaagtataa actatgcaaa taagtcgttt ttagatatgc tagcttttac
    50101 aaacatagat gatataaaag attctaatat taaactatta atgaatgcga atactgaaga
    50161 aaaaatgaaa tttacggata atatggctaa agttttaaat gaaaatagaa gtatactttt
    50221 actggaatat gaattgataa atagttatga tgatatagta gaagtagaat taagtgcatt
    50281 accttttgct atatacaata ctagatacat aatgcttatt ataaaagatt tagtacataa
    50341 gaaatattcc gagcaagcag aaaaagaact gcttgaaaga tttaaaacag ataaaatcaa
    50401 aacggagttt tttgcaaata tgtctcatga actaaaaact ccactaaatg ttatatctag
    50461 ttcaaatcaa ctggtagatt ctttctatag aaatgaaaag ataaaagatt acaataacaa
    50521 tattaaatct catgtggatt tagttagaca aagttcttat aggcttcaaa gattaataaa
    50581 taacattata gatttaacaa agatggagtc tgggttctat acactaaaac ttgcgaaata
    50641 caatattgta tctgtaatag aagacttatt tatgaatata gaagagtatg cacttagaaa
    50701 agacataaaa attctttttg atacagacct tgaagaaatt aatgtttata tagacaaggt
    50761 tgaaattgag agaattatgt tgaatctatt gtcaaactgt attaagttta ctgataatgg
    50821 aggctggata tatgtaagta ttcattataa aatagataaa gtaatcataa gtgtaaaaga
    50881 cactggagtt ggaataccac aagataaatt agagttgatt tttgaagaat tttcacaagt
    50941 tgataagact ttatccagaa atactgaagg aagtggaata gggcttgcta tagttaagaa
    51001 tctagtaagt cttcatggtg gagatataga agtagtaagt gaagtgaata aaggcacaga
    51061 gttcttaatt tcacttccaa tgaagggatt tagtagtgaa cattacacag aagataagag
    51121 aatatataat attcaagaaa aaataaaaat agaattttct gatatatatt attaaaaatg
    51181 attatgagca aaaataaaat aaaaatacta tttgtttaaa tatttaagtt gtaagaaata
    51241 ggctatcata aggtaaatta ttatgatagc ctatttttag ctaggtttta tatctatatg
    51301 tgagaacata taatctctaa attaccttca aaactaaaat ctccaaagtc gtttggtagt
    51361 ataaaatgca tacccttttt taaattgtat tctacagaat tgtgtataag ttttccattt
    51421 ccatccaaca cactgtataa agaaaaaggt gtattgtgtg taaaattaca ttttccaaaa
    51481 acatctaatt tataaacact aaaaaattcg tttgacacaa atgttgtgca tttaaagtct
    51541 gatgttgata cgattttata gtcagtatcg aaatttatat gaggaacatt cgtaacatct
    51601 attgattttt gaacatgaag ttcacgctta ttacctgaat tgtcagttct atcataatca
    51661 tagactctat aagtagtatc tgagttttgt tgagtttcta aaatcaatgt tcctttgcat
    51721 atagcatgta tagtgccact tggaacatag aaaaagtctc ccttttttat attaaccttt
    51781 ctaagtagtt tgtcccattc attattattt accatattta caaattcttt atgagatttt
    51841 gcattatgac ctattataat ttctgcatca tcagaacaat ctattacata ccaacactct
    51901 gtcttaccta actctccatt ttcatttttt ttagcatatt catcatttgg atgaacttga
    51961 actgataaat tatcatttgc atccaatatt tttgtaagta aaggaaactt atcaccaggt
    52021 gtgtttccaa ataattctct atttttattc cacaattcag atagtttttt acctttatat
    52081 tttccatttt ctattaagca atctccattt ttatgagagc ttatagccca acattcacca
    52141 gttgttggcg aatctatttc ataattaaat ttatctttaa gtgcagtacc accccatatt
    52201 ctatccataa aaataggttt taaaaataat ggttccataa tcctgttcct cctatgatat
    52261 taaatttatc ctacaaataa ttatatataa atagattata aaatcaatag taaaaaagtt
    52321 ttgtcccttg aaatgacata aagtataagg agttattaaa taagataaac tatgatatat
    52381 aatataaagt tctaaattgg agtgataatt atggagttgt ttaaggcaga aagcaaaaaa
    52441 tataaaaaag aatttttaaa attattggag caatgggtta gtataccttc attttatgat
    52501 agaaaaactg tatctaaaga catgcctttt ggaaaaggag tatatgatgc attaaactgg
    52561 tttgaaaatc taggtagaga aaacaatttc aaagtaaaaa atatagataa tcatgctgtt
    52621 caaattgaat atggaaatgg taaagagtat gtagatatat ttggacattg tgatgttgta
    52681 aatccaggtg aaggatggga tagtgaacca tttaaactaa atattatagg tgataaatta
    52741 gttgctagag gtgtatctga taataaaggg cctatgatag tgaatttttt agcactaaaa
    52801 atgataaagg atttagatat taatttaaaa agaaaggtta gattaatagc tgggggaaat
    52861 gaagagagtg gatttaaatg tataaagcat tattatagca aagagccata tggtgtatgt
    52921 ggatttactc cagatgctaa atttcctgta ttaaatggag aaaaaggtgg agcaataata
    52981 aagttaattt caaatataga cgataaatct ttatatataa gtggtggaat agagtttaat
    53041 acaataccag ataaagttta tataaagaat gtagaaaaat tgggtaaaga taatatatgt
    53101 tttgatatta acaatatatc gataaattat gataatggca attatattgt gtatggaaaa
    53161 ggtggtcatt catctaaacc cgaaaaatca ataaatccta tattagctac aattaaactt
    53221 ttgtcagaaa atattgatga aaaatggaca aaagatttat ataagttgat aaatcaagat
    53281 aatataaatg gaaacttatt tggtttaaat attgagggga aatgtggaat tttatcaatg
    53341 gtgccaacaa taataaatat agtagatgga aaattagaag tagttttaag cgttagatat
    53401 ccagaaatat taaccattga agatataata aaaaaattta atttgtatat ggagcaaaat
    53461 aatattaata aatttgagct tattggggaa aatttaaagc aagcaaatta tatagataga
    53521 aattcaaaac ttgtaagaag tttgcatgat atatatataa agtattctgg agatttaaaa
    53581 aatgatgtta gagtaactag tgcaggaagt tatgcttctg aaatgaataa ttcagtaata
    53641 tttggttgtg aatttcctga tggtagcttt ggcaatgtcc atagtgcgaa tgaatttgct
    53701 agcttagata gatttataac tgcaataggt atatatgcag aggcaataat tacattatgt
    53761 aataaaatat aaatacaaaa tgtcccttat atagacatat tatatataca attaaaattt
    53821 tacttgatat ataattgaaa tcaaagagaa ggtgatacaa tgataactaa gaaacatgca
    53881 ataatagtaa agagtttaag caataaagat ggatatatga catcaaacga attagctatc
    53941 aaattggatg tgtctactaa aactattaaa agatatatag cagatttaaa tagtgtttta
    54001 agcaaatatg atttggtaat agcttcttct agaggtatag gatacaagtt aagcggttct
    54061 aaaaacaata tagccagagc tgttaaagaa gcaaataaat atattgatgg ttttttagat
    54121 gactctgaag agtcaagaat gtcaaatata atatgtatgc ttattaatag aaattatatg
    54181 agtatagaag caatggcaga agaattaaat ctaagtatag ccgctataaa taaattgtct
    54241 agtaagctta aaagaaaact tgaaaagtat gacttagtta taaaatctaa accttactat
    54301 ggaagctata tacatggtga agaaattaat ataaggcaat taattactga ctatgcaata
    54361 aaattagatg aaaaaagtaa agtgaaggtg tttttagatg acattagtga aaacgatata
    54421 cactgtatag aaagtatttt agaaaagcac ttaagagaaa aagatacaat aatttcagat
    54481 aaagatttta atcttttgct ttcaaaaata atcgtttctg tatttaggag taagagaggt
    54541 catagtaaca atataaactt aatggataca agttatagat ttcataacta ctactttata
    54601 gaaaatctaa tgaaagaaat atctgataaa attggattta aacttattga agatgaagta
    54661 atttatattt caaattattc tggtgtaata gcctataaag gtacacaagg aagaaaaaac
    54721 acaagtgaaa ttgaggaacg aataagtact gttataagta gtgcacttca agatatattc
    54781 cttatttcag gtagtgatta tacaaaagat gatgagttta tggttgcaat ctctgaccat
    54841 ataaagagat ttttaaatag agctagagct aatgtaaaat caaataatcc tttacttcat
    54901 caaataaaag agaagtttcc tatagctttt aatttagccg tgtttttatc aaataaacta
    54961 gaaactgagt ttaatttaaa acttgatgaa gatgagcttg gatatatagc tatccacttt
    55021 gctgcttcaa atgaaagaat gaaaaaaaat actagtaaga aaatttgtat agtttgtcat
    55081 tatgggatag gaacaggaca acttatttct gaaaagctaa aacagaatat aagtgattta
    55141 agtgttgtgg gtgtttatcc agttagatat ctcgatatgg ctataagtca agatgttgat
    55201 ttgatagtat ctacagttga attaaaagga tatgaaaaac ctgtcttata tatagaaaat
    55261 atatttgatg atagcttaat tgaaaacgta aacaaagcct tttatgaaaa ggaagaaaga
    55321 agaaagataa taagtaacat gtttgatgaa aaagcttttt tcagtataaa ggctagtact
    55381 aaagaagagg tcataatttc actttcaaat aaactaaaag aaagagattt tatagaggaa
    55441 agttctataa aaagtataat agatagagaa aatatttcat caactgaaat aggaaatcta
    55501 gtagcaatac cccacacaat agtgaaggga gataaaaaat ctataatagg agttggaata
    55561 cttgaaaatc caataatttg ggataagcaa gaggttcagc tagtatttat ggtatttttt
    55621 aatactaaag aaaaacataa tttctcgata tttaaatacc tttataattt tataaaagat
    55681 gaaggaggag taagaggaat tattaaaata tgtgacttta ataaattaat ggcacttata
    55741 ggtaattaag gaggaaatta ataatgggaa caaacatatt taacaaagaa tatgtatttt
    55801 taaatgttga tgctaagtca aaggttgaag ttttaaaatt tatatcaaaa aaagctaaag
    55861 acttaaactt ggcagaagat gagagcttag tatatgaagg tttaatggct agagaagaac
    55921 aatttactac aaatttagga gaatctatag caatacctca cactaaaaat gatgcaattg
    55981 aaaatccagc agtagtagta ttgaagttta atgaagatgt tgtttggaat gaaggtgagg
    56041 ataaggttaa attagcaata agcttactta tgccaggaaa aagtaaagag aatattcatc
    56101 ttaaattatt atcatctctt tcaagaaaat taataaataa agaatttaaa gattctcttt
    56161 taaaaagtga taatgttgaa gaaatatcta acttaataaa tgaagcttta ggtttataat
    56221 taaacactaa ggttataaaa taaaaaaata attaactaaa aataggagga atttgaaatg
    56281 aataaaaaat tagtagcatt atgtgcttgt ccaatgggac tagctcacac tttcatggca
    56341 gcagaagcaa tagagcaagc agcaaaggca ttaggttatg aagcaaaagt tgaaactcaa
    56401 ggtgctgatg gtgtacaaaa tgagttaaca agagatgata tattaggagc aactatgata
    56461 atacatgcag tagcaataac accagaaggt atggaaagat ttgatggttg tgaagtttat
    56521 gaagttgaat tacaagaagc tataaaaaat gcagaaggtg ttataaaaga aatagaagaa
    56581 gatttaggaa tataataaat ttattgtgga ggaaatagaa tggcaattaa gaaaaaagtt
    56641 ataaacagtt caggtgcttc tacaacagga ggaaataatg taggaaaatc tactccatct
    56701 agtccaactc aatcaaaggg aaatggaaaa tggaaagaaa taagtaaaca tataatgaca
    56761 ggtatatcat atatgatacc agttttagta atgggtggac ttataggggc cttatctcaa
    56821 ttaataccat atgctatatt aggacttgac ccatctgtag gtatagttga tgctatgaac
    56881 tcaggagaat ttacagggtt caagcttagt cttttaaata tagcacaatt aatgtcaaat
    56941 ttcggattta ctttgtttgg atttgcgata ccactgtttg cagcattttg tgcaaactct
    57001 ataggtggaa aaacagcttt aattgcagga tttataggtg gatatatagc taataaacct
    57061 gtaggtgtac cacaattcgt tgatggacaa tggactgaag ttgttccagt tgcatcagga
    57121 tttttaggtg caatactaat agcatttata ataggatatt ttgtaaaatg gttaaataaa
    57181 tctataaaag tttctcataa ctggttagct tttaaaacaa catttttaat accattaata
    57241 gcgtcattag cttgtatggt attgatgata tttataataa ctccatttgg tggattaatt
    57301 aatgaaagta tgaaaaactt cttaacagca gcaggagcag ctggagaata tgtatatgca
    57361 acagctttgg cagcagctac agcattcgac ttaggaggac caataaataa agcagcagga
    57421 ttcgttgcac ttggtttaac tacagagaat gtacttccaa taacagcaag aacaatagct
    57481 atagttgttc ctccaattgg attaggatta acaacattat tagataaaag attagtagga
    57541 agaagagtat atgatagaca attctatcaa gcaggaaaaa cttctatatt cttatcattt
    57601 atgggtatat ctgaaggagc aataccattt gcacttgaga gaccaggttt tgttatacct
    57661 ttaaacatag ttggaagtgt aataggagct attacaggta taattctagg agcaattcaa
    57721 tggttcccag agtcagctat atgggcatgg cctctagtag ataacttatt tggatatata
    57781 ataggtatag cagttggtgc tatattcata gcagtaggaa atatattcta tagaaataaa
    57841 ttaataaaag atggtaaact tgttgtagat tatatcgact agtatttaat ttttactatc
    57901 tataaataac tttggctggt aatatggttt taatcaatat taccagcctt acctatatga
    57961 ttaattaaag gcggtggaga taagatatgg ataagataga aaaagtaaga gagtatttta
    58021 gagaatatga tatagatgga tttcttataa atagtcctac aaacaagttt tatgttggaa
    58081 atttatttag ctcttcaggt tatgttttta taactaaaga atcacaatat ataatagtag
    58141 attttagata ttttgaagaa attaaaagaa aatctagttt gtttaatgta gtattaatgg
    58201 acaaaactag aactcatttt gatataatta atgatatttg tagagagcag aatataaagg
    58261 aaattggatt tgagggaaat gaagtatctt ttgatttata tagaagtatg agtaataagt
    58321 taagtgcaac cttaaaatca gttgatttat ctacattaag ggaaacaaaa aatgaagacg
    58381 aaataaaata tataaaaaaa gcttgtgaaa tagttgatgc tacattttat catatagtag
    58441 actttataaa ggttggtatg actgaaaagc aagttgaaaa tgaaattgtt agaattataa
    58501 aagatttagg tggtcaaaaa gaatctttcg atacaatagt tgcatctggg cttagaggag
    58561 cattacctca tggaaaagca agtgaaaaag ttatagagta tggagatttt gttacctttg
    58621 actttggtgc taagtataat aattattgtt cagatattac cagaaccatt tgtatgggta
    58681 ctataaataa agagttagaa gaaatatata atatagtaag aaaagctaat gaagagtgta
    58741 taagggtttt aagaccggga atgactactg gagagataga taaagttgca agagacataa
    58801 ttggttcata tggatatgcc aataattttg gtcataatct gggtcatggt gtaggaatta
    58861 tggttcatga atatcctgca ttagctcctg aatcaaatga agtattaaaa gaaggaatga
    58921 ttgtaactat agagcctggt atatatgttc caagtcttgg aggcgtaaga atagaagatg
    58981 atgtacttat aacacaagat ggatgtatga gattgactac atctactaag gatttaatag
    59041 ttgtaagcta aaaataaatt agataaatat tttataacaa taagaaaaga agaggtaaat
    59101 ttaaatgtat aattttgatg ataaaccaga tagagtaagt gaaaagtgta gaaaatggga
    59161 tttaaatatt ataagagata aatttggaga tattagagaa gattttatac caatgtggat
    59221 tgctgatatg gattttaaaa taccaacaga aatagaaaac aaatttattg aagctgtaag
    59281 aagaggtgta tttggatata catactgtta tgatgaattt tatgattcag ttattggttg
    59341 gcaaaaaaat atgcatgagg taaatgtaga aaaagaatgg attacattaa cttatggaac
    59401 tgtatcaaca cttcattatg tagtacaagc tttttgtaag aaaggtgata gtataatatt
    59461 aaatacacca gtttatgacc cctttgaatc atcagcaaaa aaacaaggtg taaatgtaat
    59521 ttgtaataca ttagatgttg taaatgatag atattatata aattttgata aattagaagt
    59581 tcaaattaaa gaaaataagc ctaaattaat gatgttttgt acaccacata atccatctgg
    59641 aaggatatgg acaattgaag aaatgacaag ggtagcaact atatgtaaag aaaataatgt
    59701 tattttggtt gcagatgaag tacatgcaga gcatattcat tatggtacat ttaattcaat
    59761 acttaagatt ggaaaagagc ttttagaaaa tgttattcta ttgacatcac caaataaagg
    59821 atttaatctt ggtggattaa agacttctta ttcaatagtt ataaataagg atataaggga
    59881 caaatttaga aataaattaa agcaaaattc tataacttct ccaaatgtgt ttggaataat
    59941 aggattgata accgcttata atgaatgtca tgagtggtta agtggtgtaa atgaatatat
    60001 aaaatcaaat tatgaacttt tagaaacttg ggtaaataaa tataataaga taaaattgat
    60061 gaaaatggaa tcatcttact tagcttggat ggatataagt ggattaggta ttagtgcaag
    60121 tgagtttaca gataaattgg ctatagatac aggagtatta ttagaagatg gaagtcattt
    60181 tgtaaaagat ggtgagaagt atgttagaat aaatttagga actcaaaagg aaaatgtaat
    60241 tgaagcctta aatagaatgg atttattttt aaagtcttta taaaaatttg gttgaattag
    60301 tattttatat aaatttaaac tattaatatg gtatatacat aagtagaatt tgttttagat
    60361 tacttaagta tataccatat tttatataaa aattaaattt gcattagtca tgtgataact
    60421 atttaaattc atatgtattt tgtatgtata atagatataa ttattattta aacttattgt
    60481 ttatttaatt tttcaataga aaaagattta tctgtaagtt taaaattatc ttttataccc
    60541 ttagttaagt atactttctt atccttagtt atgaatatag catctacatt atcaatttta
    60601 ttaattaaat tcataccttt ttcaagacca agaccaaatg tagaagtgga taaggcatca
    60661 caattaatag atttatcaga gattattgta acactactta aattgttttc aaaaggatac
    60721 cctgtaaatg gatttaacat atgatgatat atttttccgt ctttttcgat aaatctttca
    60781 taaattccag aagtaacaac agacttattt gtagtttcta tgtttcctat ggattcacca
    60841 tttttactag ttgggtcttg aatacctatt ttaaaaggtt tatcattttt accattttct
    60901 aaagtaaata tatttcctcc taagtttact aaaccttttt ttacatcctc tgattttaaa
    60961 taggctacaa ttttatcagc agcatatcct ttagctatgg cacctaaatc aattttcata
    61021 ttttctttgg ttaattttat agatttattt ttttcatcca atatgatgtt cttataatca
    61081 ataagaggca gaagtttttg tatcttagaa tcacttggaa ctttagcttt atctgttcca
    61141 atagcccata aatttgataa tggaccaatt gttatgtcaa aatatccatc tgataatttg
    61201 gaatattgta tagcttcttt tacgacgaaa aatgtatcat cagaaactct gacaaatttt
    61261 ttaccagcat tatcatttat tttagaaaca tcacttcctg gtatatgagt gctcattttg
    61321 ttgtcaatat atcttaaaat tgaatcacat tcatttaaga ttttatcact tttggattct
    61381 tttacattaa atacagtaac ttcgtttact gtaccaaggt agtagctagt tttactatat
    61441 ttagtattct ggcttgaact cctaaaaaat gaaaataaaa ttaatgctaa aatcaatatc
    61501 acaaaaataa atgtgatttt tttcttattc atggtatatt cctcctatga aattgtgtta
    61561 ttattttatt ataacataat ttcataggag gacaaaaaat gaagaaaaaa gacattcttc
    61621 ttattttagg cattttggtt gttattacag catgttatgg gataataaat gttataaatt
    61681 ctaaaaatgc tggaaatatt gagatttatg tagataataa attgtataaa actgtttcaa
    61741 taaatgccaa agaagaattt aaaatagaga gtagaggggg gtataacata gtaaagattc
    61801 atgataaagg ggttgagata gtagatgctt catgccctga taaggtttgc gtacatactg
    61861 gatttataaa caagccaagt caaagcatag tttgtatacc taatagagtg agcataaaaa
    61921 taaatacaaa tgaaaagaat gataatcaag aagatataat ttctaaataa aataataaaa
    61981 ataattgaaa ggaattggtg ataattatgg ttaacttagg aaacgattgg gatgaattat
    62041 tgaaagaaga atttgaaaaa gattactact taaacttaag aaaatttctt atagaagaat
    62101 ataaaacacg ccaaattttc ccaaatatgc acaatatata tgaagcttta aaacacactt
    62161 catacaaaga cacaaaagtc ttaatattag gacaagaccc ttatcatgga gataatcaag
    62221 ctcatggttt agctttttca gttcaaccac aagtgaaaac tccaccatct ttattaaata
    62281 tgtataaaga attaaaagat gatttaggtt gttttatacc taataatgga tatttaatgc
    62341 cttgggcaga ccaaggagtg ttacttttaa atactgctct tactgttaga gcgcatgaag
    62401 ctaattctca taagaacaaa ggatgggaaa ttttcactga tagagtaata tctattttaa
    62461 gtgagcgtga agacccagtt atttttgtac tatggggaag taatgcacgt aaaaaagtag
    62521 agttgattga tacaagtaaa cattatattt tggaagcacc tcatccaagc ccattgtcag
    62581 caagtaaagg cttttttgga tgtaaacact tctctaagat aaatgaaatt ttgaaaaagc
    62641 taggaaaaga acctattaat tggcagatag aaaatatata aaagtaaaag gtaacaaaat
    62701 gataacaaaa caacaataaa caatgacatt gttgtaacta atagaaatat gcttaaatag
    62761 ttgaaaatac tatgtatgca tatttctatt tttttgtcaa gttgacaact cttgaaaaaa
    62821 tagttacaat ttagttacac aaaaaacaca aataaaaaaa gaaacttaca aagcagaatt
    62881 ttatgaagca aaatatatag tgtaaaaaat cttagggagg aaattttata tgaattttaa
    62941 aaaattgagt aatgctaaag tctcggtaat ggcagttgtt ttatcagttg gaatattatc
    63001 gggatactct gcgcttaata aacaagtaac attagtagta aggggagaag aaagaactat
    63061 atcaacattt agttctaatg taggagacct tttagaatct caaaacatta gctatgattc
    63121 aaatgatata gtaactttag atgaaaatac aaagttatct aatggagata aaatagaagt
    63181 aattgatgta aaggaaaaaa cagttgtaga aactaaagac attccatttg aagttacagt
    63241 tgttgaagat ggctctttat taaagggtga ttctaaggtt aaagaagaag gaaagtctgg
    63301 taaaagtgaa ttaatatata aagaaactta tcacaatgga aagaaagtag aaaaggaatt
    63361 tgtgaaaaag gttgttaaag ctgagccagt aaataaggtt gtaagcaaag ggacaaaggt
    63421 agaaatgcaa gtagcctcat caaggggaga aagttcaaga agaatggctt catcatcatc
    63481 tagtagcagt agtagtaaca atggtaaaaa tatgagagtg gtagcaactg catatactgg
    63541 tcacagtata actgcaacag gaacaaaacc taagtgggga acaatagctg tagaccctaa
    63601 ggttatacca tatggaacta aagtttatat acctcaattt gacatggtgt ttactgcaga
    63661 agactgtggt ggagcaataa aaggaaataa aattgatatc tacatgaatg attcaaaaac
    63721 agtttataac tggggaagaa aaactataga tatacgtata ttaaaatagt taaataattt
    63781 atatttttac tggaaattat attttgatta tatagagaag aaactcttta tagattctaa
    63841 aaatataatt tccagttttt tattttggat atgtaataaa acaggtaaat tttaaccatg
    63901 tatagataaa agctatttaa attataattt actatcatat aaatctataa aaattgttgc
    63961 aaagagtcaa aatatatgat attatagatt tcgttgagaa ggatttcatt atgggagtgg
    64021 atgggtgccg gtgtgccctc tagtcttcaa aactagtatg aggggttaag agcctcttag
    64081 gtgggttcga ttcccacgca ttcccgccaa tatgtaaaag gtatctttta attaaaagat
    64141 acctttttaa atttatttta agataattta tttttaataa tatataatgt tttgtattat
    64201 ataatgtaaa ttttctaata caaaacatta tatatttaga taaaaatcta ggagtgtgga
    64261 aagatggaaa ataattttac aacgagaact agttttctag ttggagatga tgggataaaa
    64321 aaacttaata attcaaatat aatagtattt ggagttggag gagttggtag ttttactgtt
    64381 gaagctttag caagagctgg agtaggcaat attactattg tagattttga tgatgtagat
    64441 ataaccaata taaatagaca aataccagcc ctacattcaa ctgtaggaag atacaaagtt
    64501 gatgtcatgg aagaaagaat attagatata aatcctaata tcaatataaa aaaaataaga
    64561 agtctttata ataaagatac tagtgacgaa atactaactg aaagatatga ttatgttgtg
    64621 gatgcgatag acatggtaag ttccaaaata catctaattg aaacttgtga aaaaaaagga
    64681 ttgaaaataa taagttctat gggtatggga aataaattag acccaactaa aatagtcgtt
    64741 acagatatac acaaaacaag tacttgtcct ctagctaaag ttatgaggaa agaacttagg
    64801 gatagaggta tcaaaaagtt aaaagtagta tattctactg aacaaccaat agaacttaag
    64861 aagaaagtaa tgaatggtag aaaagtaaca cctggtagtg tatcatttgt cccatctgta
    64921 ggaggtttaa taatcgcatc tgtaatagta aatgaactat taggacaata agttttaaat
    64981 attttaattt ataaatttta tatacaaatt tgaaggctag aagcatgaat acaaagcaat
    65041 tttatattca tacttttagc ctaattattt tatattacct tattttatgt agttgtagtt
    65101 ttatttttta gattatgtat ctatttcata attatatttt gtaaatataa tttctccatc
    65161 actataaaaa acaatatctc ctattctatc tgttctataa attgatacat ggttttgttt
    65221 tagattatta ataacctccc tatgtggatg accatattga tttttatatc cacatgatat
    65281 tgcagctata tctggactaa ttttttttat aaattctaaa gatgaagaag aattacttcc
    65341 atgatgacct atttttataa aattaatatt atttaattta aaagaatgta aaatttcttt
    65401 ttcattttct tcttcagcat cacccataaa taaaaaagaa ttatcattaa aagttaattt
    65461 gaaaactata gaatttaaat tagattcttc ttgtatatat gatggactta atacatatat
    65521 atttatattg ttatctatgt ttaaaacatc atttttatag agatgctgaa tagataagtt
    65581 tttatccgta caagaattta ttagattttg ataagcctta ctatcagttg attgttcagg
    65641 catatatatg gagtttacat taaatttttt tataatatta tcaagacttc ctatgtggtc
    65701 tgaatctgga tgagttgcta ttataatatc aatagttttt attctttttt gtctaagata
    65761 actaataatt atattttcgc tatcttcatc acctccatca attaatatat tcttatttgt
    65821 gggtgtttga actaaaatac tatcaccttg tccaacatca atcatatgta tagatagtag
    65881 agattttttg tcacagccga ttaaaagtga tattataata agtattgata aaaaaatttt
    65941 atttctcaat aaaaaatcac tccattcatt acatgtttat ataaatttat gatgggtaat
    66001 ataagatata tatgacttat tatattttat attttaaaca atataaatga aattaatacc
    66061 ataataaaat tggagttttt tatatataca aaataaaatt tttaaaatta agcaataaaa
    66121 aaggtaggag gattttatgt ttttcaaaga tattgaacta aattcaaaaa aagagttaga
    66181 cccatatttt gatttagtag attatgaagc ttgtgagtac tgttttagta ctttatatat
    66241 gtggcaacat gtttataaga caggatatta tataggtgaa gattttgccg ttttagttgg
    66301 ggaatacgaa ggagatagct tctcaatatt accacttgct aaaaaagata aacttccaga
    66361 agttgtagat tttgtccttg aatatttttc taaaaataat aaaaaaatat acttaagagg
    66421 tattactact gaagtagtag agtttttaaa agaaaaatat ccaggtagat ttgagtatat
    66481 agaagaaaga gatttatttg actatattta tgatgcagaa agtttaagaa ctctagctgg
    66541 taaaaagaat caaaagaaga gaaaccatat aaactatttc ttaaaagagt atgcaggtag
    66601 atatgaagct aaattattag ataaagaaaa ctttgatgag tgtttagtac taatgaagga
    66661 atgggaaagt aataaggaag aaaataatga atttgatgaa agtatggatg atgaattaat
    66721 aggtatcaaa aagatattta accattatga catattaaaa gataaggtaa aagtgtttgg
    66781 tgtttatgta gatggaaaat tagaagcttt tagtatagga gagcttttaa atccaaatat
    66841 ggcattgatt catatagaaa aagctaatcc cgatataaga ggtctgtatc catttataaa
    66901 tcaacaattt ttggtaagtg aatttaagga cgttgagttt gtaaatagag aagaagattt
    66961 aggtattgaa ggattaagaa aagctaaact ttcttatcat ccttgcagat ttgttgaaaa
    67021 gtacagtgtt agggaggctt aatagactat atggagatta ggtatgctaa agaagaagag
    67081 atagaaagta taaaagatat ttggagttat tgttttaacg atacggagag ttttatgaag
    67141 tactatttta atgataaata taaatctgaa aatactgtag tagctttaga tgaaggaaaa
    67201 attatttctt ctcttcagct aaatcagtat aagttattac taaatagcaa ggtatataat
    67261 acatcatatg tggtaggagt ttctacactt ccagagggaa gaggagctgg atatatgaac
    67321 aaagtaatga aatttacttt aaacgaattg tataaaaaag ggcaattagt ttctatattg
    67381 atgcctattg actatagact ttatagaaga tttggatatg agcattgtta cgaccagatt
    67441 gaatatacta ttaacacaga tgatttaaaa aattttaagt caagtggaaa aatgataaag
    67501 tctaatttat cacaaattga tgatttgata caaatagata gaactttttt aaatgaggtt
    67561 aatggtaatg tgttaaaaga tgaacattat tatgaaaact tatttaaaga gattcaaagt
    67621 gaagatggat ttttatacat tcatgaagga aatgaaaaag atggatatat agtttatttt
    67681 cttcaggaag ataaaatgtt tgtcagagag ttattttata aaaatataga tgcccttaaa
    67741 tcaatgctta aatttatata taatcacaat actcaatgta agatagttac tatatctact
    67801 cctacaatag ataagataag gtttatatta gacaacccta aagatagtga tataaaaata
    67861 aaacctttta tgatgggaag agttataaat gtaaagaagt ttattgaaga tatagatatt
    67921 gagaaggata ttaatagctc atttaattta ttaatagaag acaagtttat agatgaaaat
    67981 aatggtctat ttaaaatttc tatacagaat aagaaggtat ctgtagagca gttagataaa
    68041 aaaggtgcag aaaagcctca agaagatttt gatattaaat tagatataaa taccttaaca
    68101 cagttatcat tttcttatat agatgtaaat gaagctattt tcttaaatga tataaaagat
    68161 gtttcagaag aaacactaga aacacttaat tgtatttttt ctaaaaaaaa taactatata
    68221 aatgaatata tataggtaga gtacgttaaa agattctaaa aatcttttga cgtatttttt
    68281 tgtttagata acaatttatc ttgtttaaaa attttctatt tgaaaataat ctaaataata
    68341 tacttttata tgggagtgat tataattcaa ataagaagac cattattaat tatattaatg
    68401 tttgtggtag ctatatcatt tatcattaca aataacacaa aaaacgacga agaacttaat
    68461 gataagataa taactataca aggagttgtc aaaggaaaaa ttgaaaagaa aagatacaat
    68521 cagtacaagg taggggtttt tctaataaat gattatacaa aaaataaaaa tttgaagatt
    68581 ggtcagaaag ttaatattac aggtaaattc aaaagtttag ataaaatgaa gtatgatgat
    68641 tttgattatg gaaggtatat aaaaagtact ggatataaag gtatagtgta tataaataac
    68701 tataaaatta ttgataaaaa taaactttac tctttaatag gggaaataaa gttttatatt
    68761 agtaaaactt ataggtactt atataagaaa aactcagatt ttataaattc tatactatta
    68821 gcagaagttg aaaatttgac tgaggaacaa aaagaaattt ttaccagaac tggaactagt
    68881 catgtaatat ctatatcagg tcttcataca ggaatattat gtgtaattat atcctttcta
    68941 ttaagaggta taaataaatt gtataaatta ttaatactgt cgatttttat aactttatat
    69001 tgtataatgg taggtgcgtc tccatccata attaggtcta tagcatttgt tatgattttt
    69061 tatttatcaa tatttattga tagaaagaaa gatggaatat cggcattgtc tctaattggg
    69121 ataattttaa ttatgaataa cccctatgtt atatataatg taagttttca attgtctttt
    69181 ttagctacac tttctatatt aatattctat aataaaataa atagtattat aaaattaagt
    69241 atggtatcat tgacaatatc atcaaatata ttaactttac ctataattta ttatacattt
    69301 aaaggtatac cattgctttc aattatagga aatttaatta tagtgccatt tgtgggagta
    69361 ataatgtatt taagtattgc aagtttaatc gtatttaaag taagtgtagt aatagctaaa
    69421 attatatctt tttttaatag tacatttata gaaagcatat tttttttact agaaaaaatc
    69481 agcaatttaa gttttgcata cattaatata gaaaatccta aattttatat tgttgttatt
    69541 tattacatag gagtgttttt ttacatattt tacatagaag gaaaagagat aaaggagcaa
    69601 gagaatgaat tacaaggata ttataaggaa tgtaaaagag aaaaactttg aaaaaatgta
    69661 tcttttttat ggaagagagt attatttaat tgagaatgca ataaaagcat ttaaagatag
    69721 tctaaacgag ggaatgcttg attttaattt agatataata gatgggaagg aaatagtctt
    69781 aaatcagctt ataagctcta tagaaacact tccatttatg gatgatagga agatagtaat
    69841 aataaaagac tttgaactat taaaaggaaa gaaaaagaat tttacagata gtgatgagaa
    69901 atacttaata gagcatttag acaatattcc agacaccaca actatagttt ttgttgtata
    69961 tggagatgta gacaaaagaa aatcattagt taagaaaata ggaaacaatg gaattgtttt
    70021 tgattgtgat aagctttctg atatggattt gtttaaatgg ataaaaaaat cttttgcttt
    70081 aaatgatgtt ataatagata attctcaaat catgtatttt attgaacaag aaggatacag
    70141 agataagagt agtgaaaaga cattatctga tttagaaaat gaaataaata aaataagttc
    70201 atttgttggt aaagggaata atgtaaccaa tgatgtaatt gataaattat ctcaaaaaaa
    70261 agttgagaat gatatattca aattaatcga ttatattgga gaacaaaatg cttctaatgc
    70321 aatgaaaata ttaaatgata tgatacaaga aggcgaatct gtattaggta tattttctat
    70381 gatagcaaga caatttaaaa taattatgca agtaagacag ttacaacttg atggatattc
    70441 aacaaaactt atagctgata aattaaaaat gcatcaattt gttgtaggaa aagcattaaa
    70501 gcaaactaaa aatttttctg atgatataat tgtggaaata ttaaactata tattagaaag
    70561 tgattacaaa ataaagacag gtcttataag agatacttta gcagttgaaa tgttggttag
    70621 tagatactgt aaaagagaag caatttaaaa aatctatata ttaataaaaa ataaatagca
    70681 agatatgtta tagttcagag tagatgtaag ctaaattaag tttgatatac aatcaactat
    70741 aatatgtcta actatttatt ttttattgta taaatttaca aaataaaaaa cccctacata
    70801 ggggttatta tatataagaa atataaactg gtaattaagc gttcataccg tttaattttt
    70861 gagctaattt agctactttt ctagctgcag cattcttatg tatagttcct ttagaagcta
    70921 cttggtatat tcttttttga gcgtattgga acttagcaac agcatcctct ctgtttccag
    70981 ctgcaacagc atcttcgaat cttcttattg cagtttttat ttgagatttt ctaactctat
    71041 ttaaagcagt tttcttttcg ataacactta ttcttttctt agctgatttt atatttgcca
    71101 ccgtgttcac ctccctgtat ttttttatca tattcatcgt caacatatta gattttaaca
    71161 gaaataaatc taaaaatcaa ggattattat ttatatattt taataatgtt ttttattttg
    71221 attttttaga ctgtaaattg tgtataaata atttgcaata tcttgttttt aaattggtat
    71281 attttgtgtt ttaaatattt tgacatatat aattaatatt tcattttgta acaaatattt
    71341 gagataatac aaactattaa catacatttt gatagtattt ccaataatta taagataata
    71401 caagaaatta ctcatttttg taaatttaat gaaaaattat aaaaatataa atttatatgc
    71461 ttaattaaga taatttttcc aataatcaag ataatttttt tcattagtat gtatgataga
    71521 tgcatcaaac aatataacta atatgttaga aggttatatg taattaatgt cagtgtttat
    71581 atagaatagc atttgtagca tttattatag ttgtaattac tatttatatc atagacttga
    71641 atagtctaaa aaaatagact ataatatttt tatagactaa agaaagggct gataagatgg
    71701 acatagcaaa actaatttta aatcctgcaa ggcttcgtat tttacaatat attcgacttt
    71761 atggcagtgt tcgtacatct gatattgtaa agtatttaaa tgatattcct cgtgcaacag
    71821 tttatcacca tgtaaaaatt ttagaagaga acaatatgat tgaagtaatt aaagaaaatc
    71881 gtgtgcgtgg tactattgaa aaggtttatg cactaaagga atatacaaca tctatggaag
    71941 gagagacctt cgttgcactt tctacagcat tttatgtagg tctgatgcag gaaatgaatg
    72001 aatactttag cagaaaagag caagaccata aaaaggataa tgtatttttc tcttcagcat
    72061 tactatatat gagtgataat gaatatgaaa atcttcttaa aagtatagct gatttattga
    72121 aaccatatat agaacaaaaa cctaaatcaa atctaaaact tagaaaactt tcaattatat
    72181 cttctccacc tgtagctgat aatgataaaa caaaataaca tgcaacccat ttttataaaa
    72241 ggaggttata tatgaaattt catgagtttg gaaataaaaa cagaccacat attatgttga
    72301 ttcatggggg agggaatgca tggtggaact atttgcgaca agcagaagtt ttgtctaaca
    72361 aatatcatgt gatacttcct acattggatg gtcatggaga agaatatgaa acaacataca
    72421 tttctacaga ggatacagca gataagttga tatcatatat agatgaaaat tgcagtggac
    72481 atcttttcgc tttgtgtggg gtatcacttg gtggtcaaat tgtcatggaa cttttatctc
    72541 gaaaacctaa tattactaaa aaagcaatta ttgatggtag tatttgctac ccaagacctc
    72601 ttatggcaag gttttgtatt gctggtattc gattttttgg aggacttttg tttagtgaga
    72661 aggcttgtcg ctttcaaatt gctatgatgc caaaattgtt acctgaaaat atgcaatatc
    72721 cagatgaaat aaaagcgtat tatatgacag atatgccaca tgtaagaaaa gaaacattct
    72781 ataatatgta tcgtacttac atgatgaact atacacttaa agagaatgtt aaaaaaacca
    72841 cagcacaagt aatgtactgg tatggagaaa aagaaatgaa gtgtgtcaaa aaatctgcaa
    72901 aactgtttca gtcttatgtg ccctcttgtg agatttatga agctaagagc tataatcatg
    72961 ggtatttatc actttatctt ccaaatgaat ggttggaaat tgcagaacca tttttccaaa
    73021 aggaataaag gtgttaggtg attattgtat aattattgct gtttttttat atagtaaata
    73081 aattaggtat aaatataaaa aattatttta gttaatatat tagtgaaagc ttaataggaa
    73141 aattataaat agattaaagg tagcacctat ataaatagtt gctacctttt tctgtttgta
    73201 aataaatgat agataagaag gtttactgac aaatcactga ctaaacccaa ttttcttaca
    73261 ttgaaagcct taattttact tgttttaaat aaatttacaa tttcttgtta taaaatacta
    73321 aattttgtta acaattataa ttagcattta atttatactg gaggtttata tgattagtgt
    73381 aagaacagat ttagctttag aagctagtga aatgtgtgaa aagtctcaag aaggaagtag
    73441 tattcctggt gtaaaaatag aaactaaaga attagaaaat tgcattgtaa ctaaagttga
    73501 agtaatagac aggcaaggcg cagagataat gaataaagat ataggaaagt atataactct
    73561 ggaaagtaat cttatgaagt ttgatgatga tgagtcaaga gaagaaatga taagttattt
    73621 aaaagaggag ttagtagata tatttggcca agacaaaaat aaaaaaaccc tggtaattgg
    73681 tcttggaaat agaaatatta cttctgatgc gttaggacca aagagtgtat caaaaacact
    73741 tgtaactaga catcttttta aaaattataa taaagactat gatgatgatt tcactgaggt
    73801 atcagcttta agcccaggag ttatgggtgt tactggaata gaaactagtg aaatagtaaa
    73861 atcattggtt gagaaggtta agccagatag agtagtagca atagatgcat tggcttcaag
    73921 aaaaatggag agagtaaact ctactataca gatatcaaca gcaggaatat cacctggtgg
    73981 aggtgttggc aatactagaa aatctctaac taaagaaact ttaggcgtgg atgttattgc
    74041 aataggcgtt ccaacggtgg ttgatgcagc aactcttaca atagatgtct tagatatggc
    74101 aatagacaat ttaatagctc aatcagaaga aacagaaagt ttttatgaaa tgttgaaaaa
    74161 acttaaagaa gaagaaaaat atcatctaat aaaagattcg ttagacccat atgacaagaa
    74221 cttaatagta acaccaaagg atatagatga tacaatcgaa aatctatcta taataataag
    74281 tgaaggctta aataggtctt tacatccagg tagattggta taaaataagg aggagcaatt
    74341 atgtttaaaa aatgcattaa agtagtaacc ttgactttta tattagtatg cattttacca
    74401 ggaaaatcat tagctttgaa tcaagatgat tttttaaagt ttttagtaaa ttcatcttat
    74461 ccagaagcta aagtcgaagg aaatgataca gaaaataaaa agaataataa aaataaagaa
    74521 acaagtaagg aaaataagga agaaagtaaa gaaggaaaca ctaaatctaa agatgcaagt
    74581 aaagttgata ataaaaaaga gagtgaaaaa gaatatataa aactttatgt tggtaaagaa
    74641 aatgttccag atattgagtc aaaaaattca gacacaactg aaactaacac aacatctagt
    74701 tcagattata aggatgattt aagagtaacc aaagaaaatc caagaatttt gatatatcat
    74761 actcatggat gtgagactta ttcaaattca cctgatggaa attatcattc aagagataag
    74821 aaaaattcag taatggaagt aggaagtgca ttaactagtg ctttagatag caaaggatgg
    74881 ggtgtagtcc atactactaa ataccatgac tatccatctt ataataactc ttatgcaagt
    74941 agcttaaaaa caatacaaag tatattacct aaatataact cagtggatat agcgatagac
    75001 ttacatagag atgctaggga tttaactaat ccagccacta aggaaaaaga ccatttaaaa
    75061 tatacaacta tgataaatgg agagagagta tcaaagttct tctttgttgt aggaggtaaa
    75121 aatacaaata gaaaacaact tagagcttta gcagaagata taactgcttt tgctgagaaa
    75181 aagtatcctg gtctagtatc tccaatagta gaaaaagact atgctagatt caatcagttt
    75241 gcagttaaga atcatatgtt agtagaaata gggaataacg caacaagtgt agaagaatct
    75301 aaggctacta caaagtattt agccgagatt ttggatgaat attttaaaca aaaaaattag
    75361 agctttaagt tataattagg agcatgctaa atgagccgat tagaaaaaaa tatagaaagt
    75421 aaaagtaaac taagaaaata caagtatatt atgagaatct tatttttgat tactatgatt
    75481 atatgtacat cagtttgtgt atttatagtg gacagtaatg caaaaagtat gctaggaatg
    75541 aaacaatatg attttccaag tattaaattt gatataacaa aatttgattt tgacatacca
    75601 aattttgata tacatgaaat cataaaaaaa tttaataagt agtaataagt gaataaggtt
    75661 tattgattaa aataagcctt attttagtta tatattttta atgtataaaa taaaaattca
    75721 ttaactgtat tttataggat ttactttaag tttatgatat tatataatat ttagtgttag
    75781 acttgataat ccacatataa tgtggataat tttgtttagt gccaattagt ttatattcaa
    75841 aaattttaaa tttggtatat aaagagtttt tagataatgt aaaataataa ctataagaat
    75901 aatcaagagg agggaatttt aaggtggaca ataaacaaag tagaactaga aattttagta
    75961 taattgcaca tatagaccat ggaaagtcta ccttagctga tagattaata caacaaactg
    76021 ggcttgttag tgaaagagac atgaaaagtc aattacttga caatatggac cttgaaagag
    76081 aaagaggaat cactataaag cttcaaaaca taagattaat gtataaagcg aaggatggaa
    76141 atgaatatta tttaaatctt atagatacac ctggacatgt agactttaac tatgaggtat
    76201 caagaagttt agctgcatgt gaaggagcac tattagtagt tgatgcagct caaggtgttg
    76261 aggcacaaac tttagccaat gtttatcttg ctatagacca agatttagaa atattaccaa
    76321 taataaataa aatagattta ccaagtgcaa gaccagagga agtaaaaaat gaaatagaag
    76381 atttaatagg gcttgattca agtgaagctc ctcttatttc agctaaaact ggtttaaaca
    76441 tagaagatgt tttagaagat attgtaaaaa atgttcctcc acctaaagga gataatgaag
    76501 ctccattaaa ggcattaata ttcgattctt actatgatgc ttacaaaggt gttgtagctt
    76561 atgttagagt atttgaagga actgttaaaa agggtatgac aataaaaatg atgaatacca
    76621 ataaaaagtt tgaggtaact gaagttggtg ttatggctcc aggtcaaact gaacttagtg
    76681 aattatcagc aggtgatgtt ggttatattg ctgctagtat aaaagatata agaagttgtc
    76741 gtgttggtga tacaataaca gattcaaata atcctacaga agaaccttta ccaggataca
    76801 aaaaagctac tccaatggta tattgtggta tatatccagg agaaggagaa aagtatgaga
    76861 atgttagaga tgctcttgaa aagcttcaag taaatgatgc tgcacttgaa tatgaagctg
    76921 agacttcagc agcactagga tttggtttta gatgtggttt cttaggtctt ttacatatgg
    76981 agataatgca agaaagactt gagagagaat ttaaccttga tataattact acagctccat
    77041 ctgttatata tagagttaca aaaatggatg gagaggttgt aatgatacaa aatcctgcaa
    77101 acttaccaga accatctgag ataaagatga tagaagagcc tatagtaaaa ggagatataa
    77161 tagttcctaa agactatgtg ggtgttgtta tggagctttg ccaggaaaga cgtggaaata
    77221 tgttaaatat ggaatatata gatgatagaa gagtaatgct tcactatgac cttcctctca
    77281 atgaagttgt ttacgatttc tttgatgctt taaaatcaag aactagaggt tatggttctc
    77341 ttgattatga ggttaaaggt tatgtagcat ctacacttgt aaaacttgat atattaatca
    77401 ataaagagca agtggatgca cttagtttta ttgtccatga gacacgtgct ttccctagag
    77461 gaaaggcaat gtgtgagaaa ttaaaaggag aaattccaag acatcagttt gctataccaa
    77521 tacaagctgc tgttggtaat aaggttattg caagagaaac aataagcgct ctaagaaaag
    77581 atgttcttgc taaatgttat ggtggagata tatctcgtaa gaagaaactt cttgaaaaac
    77641 aaaaagaagg taagaagcgt atgagacaaa ttggttctgt tgaagttcct caaaaagcat
    77701 ttatgtctgt gcttaaatta gatgagtaaa tccttataaa tttagataaa gatatttaaa
    77761 agctagagtg attttagaat tatttctaaa ttagtctagc ttttttatgg tatattttaa
    77821 gtaaactcat agagaatata agtaagaata ttaaagaaga ataaaaaggt gatatttatg
    77881 attaatatag aaattcctaa aaaagtagac tatataatta aagaattaga aaagaatggt
    77941 tatgaagctt atatagttgg aggatgtgtt agagattgtt tattagaaag agttccaaac
    78001 gattgggata taacaactag tgctagacca gaagtggtag tagaattatt tgaaaaaaca
    78061 attccaactg gtatacaaca tgggactgta acagttatga ttgaacatga accatttgaa
    78121 gtgacgactt atagaattga tggaaattat agtgatggta gacatccaga ttcaatagaa
    78181 tttacaaata acatagttaa ggacttgtca agaagagatt ttactatcaa ttcaattgca
    78241 tacaattcaa agacaggatt agtcgaccca tttaatggct atgaagatat acaaaataag
    78301 tatatacgtt gtgttggaaa tccagtagat agatttgaag aagatgcact tagaatgctt
    78361 agagcagtta gattttcagc acaactcaat tttaaaatag ctgaaggtac taagcaaagt
    78421 attcataaaa aagcagattt gataaaaaat gtttctattg aaagaatcca gacagagttt
    78481 aataaaatat tagtatctga ttcatctaaa ttaaacttat taaaatcaac aggtttgttg
    78541 aaatttataa ttcctgaaat atgtgaatta gaagatgtta ctcaacacaa tccatatcac
    78601 atctatgatg tacaaaaaca tactttaata gctacagaag taattgaaga tgaactgtat
    78661 ttaaaactta caatgttgtt tcatgattta ggtaaaaaag taactaaaac aactgataaa
    78721 aatggtgttg accattttta tactcatagt agagaatctg taaagattgc aaagaagatt
    78781 ttgaaaagac ttaagtatga taattatact attaataaag ttttaatatt aatacaatat
    78841 catgattata gaatagaacc taaaaggaag ataattaaaa agttattaaa taaacttgaa
    78901 gatgttgaat tatttgaaga tttaataaaa gttaattggg ctgatacact tgcaaaaaat
    78961 ccaaagtatg caaaacaaaa gatattaaat cttattgagt gtgaaaaaga atttaaacat
    79021 ataataaatc aaaaggagtg ctttaatctt aaagatttag ctattaatgg aaaagatttg
    79081 atatctatag gtataaaacc aggtaaagat ataggatata ttttaaataa gatgcttgaa
    79141 atagtcataa ataatccaga gttaaatgaa aaagaaatac taaaagaaaa agcattaaat
    79201 atatatacat tttagattca aatataaaaa atttaattac tttaaaaaac atagaataaa
    79261 atacttattt agataaaata aataattttt agagaatatt ttagaagtta cgattgtatg
    79321 atttttaaat gcataggcat ttactctagg actatagact aatataaaaa aaaactttgg
    79381 gaatactaaa accataaatt tataaaatta atattttata tactgtaaaa cagtgaggag
    79441 ggacagtatg catactcaac tacaaaagac tattggtctt tcagcagctc tttctactgt
    79501 tgtaggtatg gttataggtt cgggagtttt ttttaagcca caagcaatat acactacaac
    79561 taatggagct ccaggtctag gtattatagc gtggctttta ggtggtttta taacaataac
    79621 tgctgggctt acagctacag aaatttcagc tgctatacca aaaactggtg gtatgatgat
    79681 ttatatcgaa gaaatatatg gagaaaagtt aggtttttta actggatgga tgcaaactgt
    79741 attatttttc ccaggtacat cagcggcatt aggagtaata tttgctcaac aagcatctga
    79801 acttttagga atgagcccaa ataatatggc aaatgtactt cctatagcaa ttggagttat
    79861 actattttta gcgttattaa atataatagg ttcatcctta ggaggaaaag ttcagacagt
    79921 agccactatt ggaaagatga tacctttaat tttaataata gtctttggat ttattaaagg
    79981 tcaaagtagt gaagtattaa atccatttgt aggagatggt gtaaatgcat caaatgcact
    80041 tggacaagta ttaatagcta ctttgtttgc atatgatggt tggataaatg ttggagctat
    80101 atcaggagaa atgaagtctc cagagaaaga tttaccaaga gcaatagttg gtggtttatc
    80161 attagttatg gcagtttata ttataataaa tatagcttat ctttgggtag taccagcaag
    80221 tgaattagca acagttactt ccccagctac attagtagca acaagattgt ttggaaatat
    80281 aggtggaaaa gtaataactg tgggtatatt aatatcagta tttgggacat tgaatggata
    80341 tttattaaca ggttcaagga taccttatac attagctgaa atgggaactt taccagcatc
    80401 taaaactctt ttaaaagtaa attctggagg ttctcctgta aattcaatac tacttataac
    80461 tgtgttagcc tgtgtatatg cattatctgg tcaatttaat cttttgacag acttaacaat
    80521 attttcaata tgggtatttt atgtattgac ttttataggt gttatgaggc ttagaagaca
    80581 aaagccagat ttacacagac catataaagt ccctttatat ccaataatac caattatagc
    80641 tatattaggt ggactgtttg taataataaa tcaaatattg acttcaacag tcatatcttt
    80701 aggcggaata tttataacac tattaggttt accagtttat tattatatga aaaaaagata
    80761 actacttaat ttaagttaga taaatttggg taaaggtgaa aattaacttt tatccaaatt
    80821 ttttatactg ttatacaata taagtattat atattgcttg atgtgctatt ttgtttgatt
    80881 tataatttag taattgatgt tttaagataa tttaagaaat gaggagtgaa agatgctagg
    80941 actttatgta catattccat tttgtgtgaa aaaatgtaag tattgtgatt ttaattctta
    81001 taaaatggac atagattcaa aaaaaagata tatagaagac ttaaaaatag aaatggaatt
    81061 atatagtaat aaattatata aagataataa atataaaaat aaagaatgct gtagtctaaa
    81121 taaaaatgac aaaataacga gtatatttgt agggggaggt acacctagta tactaacctc
    81181 tgatgaaata agagaagtat ttataagtat aaaagaaatg tttgatatag atgagaatgc
    81241 agaaataact atagagtgta atcctgggac attaacatta gaaaaattaa aaactatgaa
    81301 agagattggc ataaatagac ttagtatagg gctacaagct attcaagaaa agcatttgaa
    81361 ctttatagga agaatacata cttatgaaga atttgagaaa aactataaag atgcattaag
    81421 tgtaggtttt aaaaatatta atatagattt aatgtacagt cttccaaatc aaactctatg
    81481 tgattggaag gaaactcttg aaaaagttgt acacttaaat ccaacacata tatcagctta
    81541 ttcccttata ctagaagaag gaactgaact atacaatatg tatgaaagta ataaatttga
    81601 acttatagat gaaaatgtag atatagaaat gtatgagtat acaataaact acttaaagtc
    81661 aaaaggatat aatcaatatg aaatatccaa ctactcaaaa gaaggctata attgtgagca
    81721 taatatttta tattgggaat gtgaacatta tatagggatt ggagcaggag cttctggcta
    81781 tattaatgag aacagataca ataatgtaga atctttagaa gattatcatt taagtttggt
    81841 aaaaagagaa aaaccaatac aagaaaatga aattctttct gagaaagata tgatagaaga
    81901 aaagatattt atggggctta gaatgaataa aggcataaaa tttgaagatt ttaaaaaaaa
    81961 atttggaata gattttagag agaagtataa taagcaaata gaaatgctat tagctagaaa
    82021 gcttataaat cagagctttg aaggtataca attaactcaa aaaggaagag aaatatctaa
    82081 tagtgtattt atagaattta tggaataatt tttaaggtgt tgacaaaata aatttataat
    82141 ggtatataat atatataatc tttttagcac tcgaacttag tgagtgctaa cagctaggag
    82201 gtataataat atggaattaa atgaaagaaa attaaatatc ctcaaagcta tagttaaaga
    82261 ttatatagaa acagctgaag ctataggttc tagaacaata tctaaaagac atgatttagg
    82321 tgttagtgct gctaccataa gaaatgaaat ggctgacctt gaagaattag ggtatttaat
    82381 tcagcctcat acttctgcgg gtagagttcc atctgaaaaa ggctataagt tatatgtaaa
    82441 ctctcttatg agtaaaagcg aattggatga taatgataag attcttattg aacaatgtat
    82501 gaatcacaat ataaatcata taaaagaatt gattcatgaa acttctaagc tattatcgca
    82561 actaacaaac tatacaactg tagcagttac taagagttta ataaatcaaa gtgttataaa
    82621 gcacatacag ttggttgcta tgaatgacaa taatatttta cttatagttg taacagataa
    82681 aggggactta aaaaaagcta accttacaac taatgtatat ttagatcaat ctaagttaaa
    82741 tttgatttct gataatctta caaggaagct cttaggtaaa agtataacag atttggatga
    82801 taatctaatt gcttttatta aatacgaaat aagtgagtat tctgggctta tagatgagct
    82861 tttaaatgct ttgaactcaa atatgaaaga agaagatttt tctctatcat taaatggagc
    82921 tactaacata tttagttatc cagaatttaa tgatgtatta aaagcaaagt catttttaaa
    82981 tatgttggaa aaaaaggaaa caatagctga cataattaaa tcaaagggaa tacaaaaaga
    83041 caatctaaat ataataattg gtagtgataa tgattgtgaa ctggcacagg attgtagtat
    83101 agttacagca acttataatg tagatagaga cttggttggg cgaataagct ttataggccc
    83161 taccagaatg gattatgcta gaatttactc tataataaat tatatgagtt tattaattaa
    83221 tagaaagtaa agaaatttta aattgcgagg tgtaaagtct tggataaaaa aaatgagcaa
    83281 caggaagtaa gagaagaaaa tgatacttct attaaccaag aatcggaaac tcaagtagag
    83341 ttagaagagg aagttgttaa tgaagaatgt gaaacatcaa gtgaaaaaac tgatgaaaaa
    83401 gaagttgatg atgaaaatgt aacagatata aattctaagt tagcagaaaa aaaattgcag
    83461 gatgagttgg atgaattaaa tgataaatat caaagacttc aagccgaata tgcaaactac
    83521 agaagaagaa ctcaacaaga gaaagagaca ataggcgtat ttgcaaatga aaaaataata
    83581 actgaattga taccagttat agatagtatg gaaagagcct tagatgcatg tgaagataag
    83641 gaagatacta tgtataaggg tataagcctt gtacataaac aattaataga cactttagta
    83701 aaatttggag tagaagaaat agaagcagaa tctaaggaat ttgaccctaa tctacatcta
    83761 gcagttatgc aagaaagtgt agatggagtt gaagcaaatc aaatagtaat ggtacttcaa
    83821 aaaggatata agctaggaac taaagttgta agaccttcaa tggttaaagt ttcttgctaa
    83881 gtataaatta aaggttaaat tatttaacct aatataaaat attaaaataa gtaaaactta
    83941 attatataat tactcgagga ggattttagt catgggaaaa ataataggaa tagatttagg
    84001 aacaacaaat tcatgtgttg cagtgttaga aggtggagaa gcacaaataa tagcaaatag
    84061 tgaaggtatg agaactactc catcagtagt agcatttaca aaagatggtg aaagaatagt
    84121 tggagaacct gcaaaaagac aagcagttac aaatgcagat aaaacaataa cttctataaa
    84181 aactcatatg ggaactgatt ataaagtaaa tatagatgga aaatcatata ctccacaaga
    84241 aatatcagca ataatattac aaaaattaaa atcagatgca gaaagttact taggacaaac
    84301 agtaacagaa gcagttataa cagtaccagc ttactttaca gatgctcaaa gacaagcaac
    84361 taaagatgct ggtagaatag cagggcttga tgttaagaga ataataaatg agccaacagc
    84421 agcagctctt gcttatggta tggataaatt agaccaagaa aagaaaatat tagtatttga
    84481 cttaggtgga ggaacttttg acgtatctat acttgaaata ggagatggaa cttttgaagt
    84541 tttagctaca gctggtaaca atagattagg tggagatgac tttgaccaaa tagtaataga
    84601 ttatttagca gaggaattta aaaaagctga aggtgtagat ttaagaaatg ataaaatggc
    84661 tcttcaaaga ttaaaagaag cagcagaaaa ggctaagaaa gaattatcat caacaatgag
    84721 ttcaaacata aacttacctt tcataactgc tacagcagaa ggaccaaaac acttaaatat
    84781 agatttatca agagctaaat ttgaagaatt aactagaggt ttagttgaaa aaactatgga
    84841 accaactaag acagctttac aagatgcagg actttctaca ggtgatatag atgatgtatt
    84901 attagtaggt ggttctacaa gaataccagc agtacaagaa gctgttaaga agtttatagg
    84961 aaaagaacct cataaaggta taaacccaga tgagtgtgtt gcagcaggtg cttctataca
    85021 agctggagtt ttagcagggg atgttaaaga cttattatta ctagatgtta ctccattatc
    85081 tcttggtata gaaactatgg gtaatgtaat gactaagata atagaaagaa atactacaat
    85141 accaactaag aagtctcaaa tattctcaac tgctgctgat aaccaaacag ctgttgatat
    85201 acatgttctt caaggtgaaa gaagtatggc ttatgacaat acaactttag gaagattcca
    85261 attaacagat ataccaccag cacaaagagg aatacctcaa atagaagtta cttttgatat
    85321 agatgcaaat ggtatagtta atgtttcagc taaagattta ggtacaggaa aagagcaaaa
    85381 aataactata acatcaaaca ctaatttatc agaatcagaa atagagcaaa agataaaaga
    85441 agcagaaatg aatgcagaag ctgacaaaca aaagaaagaa aaaatagaag cattcaatca
    85501 agctgaatct actatatatc aaactgaaaa aactttaaat gaacttggag ataaaataag
    85561 ttcaggtgaa aaagaggata tagaaaaagc tatagcagac ttaaaagctg taaaagataa
    85621 tcaagatgct acagcagaag aacttaagaa agctacagat gaagtaatga ctaagttcca
    85681 aaaagtatct caagaaatgt accaaaaagc agctcaagaa caacaagcgg cacaaggtgc
    85741 agaacaagca caagacaatg gacctaaaga tgacaatgta gtagatgctg actttaaaga
    85801 agtagatgaa gataaataag ctatataata tgtaacacta aatgataatt ggaacatata
    85861 tgatttgtga aaatcatata gatgttaaag atatatggac aatatacatc ataaagaata
    85921 taatatatca tagattatat aacttaaagt agaatataag aattaatgat agcttgtagt
    85981 ttattattct acaagctatc gttttgtaaa atgaaggtgg tgtcaatttt tgagtacaaa
    86041 aagggattat tatgaagtct taggtatcag taaaggtgca gaagctcaag agataaaaaa
    86101 agcttataga aaattagcaa tgaagtacca tccagataga aatcctggag ataaagaggc
    86161 ggaagaaaaa tttaaagaga taaatgaagc ttatgaagtt ctatctgatg atacaaagag
    86221 aaaaacttat gaccagtttg gacatgatgg gctaaatggt caaggcggtt ttggtggtca
    86281 aggaggattt ggcggtcaag gctttggtgg ttttgaagat atgttcggag atatatttgg
    86341 tgacatgttt ggaggaagct tcggaggtgg aagagcaaga agaagagggc ctcaacgtgg
    86401 tgcagatata agacagagtg ttactatatc ttttgaagaa gctgcatttg gtaaaaagat
    86461 gtctataaaa gtaaatagaa gtgaagaatg tgaagaatgt aatggaactg gagcaaaacc
    86521 aggaacatca aagaaaactt gttcaacttg taatggaact ggtcaagtta gaactgttca
    86581 aagaactcca tttggtaata tagcaagttc aagaccatgt agtgcttgta atggaactgg
    86641 agaagtgatt gaatctccat gtagtaaatg tcatggaact ggaaatacaa gaaaagttaa
    86701 gactatagaa gtagatatac ctgctggtat agatgatggt caaatgataa aactttctgg
    86761 tcaaggtgaa gtaggagaaa aaggtgctcc aagaggagac ttatatattg tagttaatgt
    86821 taagtcacat ccactattta ctagagatgg aaatgatatt tattttgaaa tgccaattac
    86881 atttgttcaa gcaactttag gtgatgagat agaagtacct actttggatg gaaaagttaa
    86941 atatagtgtg ccagagggga cacaaactgg aactgtattt agacttaaag agaaaggaat
    87001 ccctagaatt agaggtaatt caagaggaga ccaatatgtt aaggttgtag ttgaaattcc
    87061 taaaaaatta aacgataagc aaaaagaact attgagagaa tttgcgaagg agtgtgggtc
    87121 gaacgtccac gaaaagaaga aaaccttcgg acaaaagata gaagatatgt ttaagaaaaa
    87181 gtagttaaag tagccaatat ataatcatat ttttaaaatc ttagttattt ataagattgt
    87241 caaaggagat attatgattt ttatattggc tatttttgta taaaaaataa atgtgcataa
    87301 aataaaactg aatataaata attttctgaa aaatacttgt cctaaaatat ataatatgct
    87361 ataatataat caaataaaag aaaggggtga ttccagtggg aacggatgtg gcactgccaa
    87421 atgatgaaga aaactttatt gtaatataca aaataaaatt agatactttt agtgctagat
    87481 taagtactgg aaaaactcat gagctgggat aattattgta attacctaga ctcaaacttt
    87541 tagtagtaaa atagtaatat tatatagaca aggtatctaa tttttaaatc ttttgtaaga
    87601 attaaaattt ttataaaggg tatatctgat tagaatgtaa ttagggaaac ctagttgtga
    87661 attactttta gtatatacat gtgtaaagaa atatgtatat ggtattgggt attgtgctag
    87721 atgtgtatgt aaaggaatat gtgtctagtt gggtatatgt aaagaaatat acttataatc
    87781 tatgaaaaat ttttagtata aattgctaaa acattgagat tgttttttat agaaagttag
    87841 tttataccaa ctaaaaatgt gcttaagatt tgtttgatgt ttaattataa ataaggttgt
    87901 agataaaaaa ataagtaaag acaaaggttt aaaatacttg ctttaaatat agtttgtttt
    87961 gaacacttgc caaattaaaa taattaataa aatagacatc ttctcatagt aaaatatact
    88021 gtgagaggat gtttttgctt tgttcataaa cttttatgaa aaatttgcat agtgtcacat
    88081 tttaatatac tatatatata ataaaatata gataaaatta aatgattcaa aagagaaagg
    88141 tgaaaagaga tgtattatat aggtgtagac ataggaggaa caggtataca agcaggagta
    88201 gttgataact atgggaagat aatttttagg tcagagtgta aaacagtaat tgaaaaagga
    88261 tttgaaggaa tacttaatga cataaaaata atgatttata agttgttgga agataataaa
    88321 cttactatga gtgacataaa atctattgga tttggtgttc caggttttat aaataaagaa
    88381 ggattagtaa cttgtgtaaa tttaaaatgg aataaaaaag cttttaataa agagttaaaa
    88441 aaaagatttc cagatgttga aattcatgga gaaaatgatg caacagtagc agcacttgga
    88501 gaagcaaagt ttggaagtat gaaaggtgct aatgttggag tattatatac tttaggaact
    88561 ggtgtaggtg gaggaatagt aattaatcaa aaagtctttt ctggagcaca tggattgggt
    88621 tcagaaatag gtcatcaaat tataggagaa aactatttta attgtaattg tggaaataat
    88681 ggttgtgtag aaactttttg ttcagcaact gcaattataa agtattctca aaaactgata
    88741 gaagaaggag aaaagagtag gattttagat ttggcagagg gaaatttaga aaatgtaaat
    88801 gcaaagatgg tgtttgatgc atatagagaa aatgatttag tggcaattaa agtaataaat
    88861 agatttaaag agtatttagc aaaaactttt gcaaacacaa taaattcttt agacccagaa
    88921 attatatcta taggaggagg catttctaaa tctagcgata taatacttga tggaatagaa
    88981 ggtttggtaa gaaaatttgt attatacaaa acagaagata tagctacaat tacatgtgca
    89041 actttaggtt cagacgcagg aattatagga gcagcttttt tataatgtaa atgaactgct
    89101 tattggaggc gtgatgatat atgggtaaaa caaatgctag aagaacgctt tttttgatag
    89161 caataggttc aggaactatg ctgaatcctc ttaattcttc tatgatttca cttgctttac
    89221 atagtataca aaatgatttt catattagtt ttacaactgt atcttggatt gtttcagcat
    89281 tttacctagc tagtgctatt gcacaaccag tttcaggtaa gataggagat ttaataggta
    89341 gaaaggtctt atttttgagt ggtttgatgc ttgtaatttt atcagcatcc atagtaccac
    89401 ttgttcaatc attctttata ttgattttta taagagtaat tcaagcaatt ggaacaagta
    89461 ccctatatcc atctggagtt gctcttatac aaaataatat aaaagaaaga caatcttctg
    89521 cattagcagt tctaaccatt tttgcttcaa ctacagcagg attaggtccg acacttggag
    89581 gattattgtt ggacttagga ggatggcatg ctatatttct tgtaaatata cctgttgttt
    89641 tggtttctat atgcttaggg tattttttat tcccaaaaga agtaaaagaa aagaaatcta
    89701 taaaagaaac tttaaaaaat gttttgtcaa gattagatat tattggcata cttctatttt
    89761 caattgcaat gatttttata ttactatttt tgctatctat gaaggaatct tttaacttag
    89821 aacaacttgt atttggtata atactgatgt gtctttttat ttggcatgaa ttgagaacaa
    89881 aattgccatt tatagatatt aagctttttg taagtaatcc taaattgtca aaagtttact
    89941 tgcaatttat tatattaaat ttctttaatt atattttgtt ttttggtctt cctagttatt
    90001 ttcaagatgc cttacattat agtgcaaagt cgactggatt atttatgcta tttatgtctg
    90061 gtataggtat atttatatct ccattaactg gaaaatggat agataaatct ggaactagat
    90121 ttcctcttgt tacatcatca atctttatgt ttattagtgc agtacttctt tcagtagttt
    90181 ttatccatat acctgtaata ggaaagggta ttatattatc attagcaggt attagttatg
    90241 gagttggtaa tgtagccctg caatcctcta tgtttgaaga aagtcctcgt gacagtatag
    90301 ggacagcttc tggtctgttt caaactagtc gttatttagg ttctatttta gcaactgttt
    90361 tacttggaat ggtatttgaa gtaaatataa catcagaaca atttcagata ttaggttatg
    90421 ttatggtggt cttaggtaca ataagttttt tattaaattt tatattaaag aaagaattaa
    90481 gaaaagtaga atagtggatt tatggattta ataagtctaa taactgataa gtttaaaaat
    90541 attgtttgta aaacataact ggtttgatat ttattaattc taagttttga agcatagagt
    90601 actcgataag tatacaatat tttgaattaa aattaagcag aaatttgaac tagtctttca
    90661 aatctctgct taatttagta gttaaatttt tttatagaga aaataagatt tgacacttag
    90721 ataaaaaatc attgcatttt caaaattctt taaatcaaac ccaagtatct cattaatctt
    90781 ttccaatcta tatactagcg tgtttctatg taaaaacata ctagtagatg tcttagaaat
    90841 atttaaatca ttatttataa attttattcc tgtatataga agttcttcgt caatagattt
    90901 aatcttatta aaatc
//
LOCUS       DAEPIN010000008        87540 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.008, whole genome shotgun sequence.
ACCESSION   DAEPIN010000008 DAEPIN010000000
VERSION     DAEPIN010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 87540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 87540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..87540
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.008"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            complement(<1..1181)
                     /locus_tag="KR250_000690"
     CDS             complement(<1..1181)
                     /locus_tag="KR250_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0066655.1"
                     /translation="MKYKIGFLTTSYFMSDKFKQALKELNDTCEITFIELKGENPFNN
                     LPNIYYEQVDNFDGFVCSGIVPYSELIINIKDIKVPLNFLKLDERDFYKYLFKLLNSQ
                     KDIDFTKSFMDFLREDNNYYDIYSLIDSNKCPYTIKDFNIPQPVYDFKKLNDKLLEIH
                     LDLLRDNKINLSFTRNYIISCELNKLGYDCIYMVPSTESILNTFKSLVNEITLQNLDK
                     NKSATCIVTVNSSEYINEDLIFKNDEIQNQIYNTILNSLSRHGFYGVQVKKNDMKIEI
                     HTTKEMLNNMTNNYTDFFISEDLKEIKYSLNIGWGIGNTNIHAEQNARQANGKSASLN
                     GNCTFIVNDTNDTIGPLYSNKNSNNIEDSSIANKVASFIPLSNTNVSKIMCMINDRNS
                     N"
     gene            1449..2621
                     /locus_tag="KR250_000691"
     CDS             1449..2621
                     /locus_tag="KR250_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBH0066656.1"
                     /translation="MNTYLNEISKFVDENREEIVSLWKEIVNIESYTHCKESVNKLAK
                     RLKFEFEKEGLDCELVDVGDNGSTLIGTLGSNINKKPIIFSGHMDTVFETGTFGENPF
                     KIIEGKAYGPGVLDMKGGIIISLYVIKALNKIGYKERPIKIVFSGDEEIGHKDSTGAD
                     VILREAKGGLCAFNMETGLVDNSLCVGRKGRIGCNIHVKGVETHAGNDFEGGRNAIEE
                     MANKILRIQKLTNLDVGTTVSVSVIKGGRVENSIPEDCSIKVDLRFEKVEEMENVKEQ
                     VREICKETYIEGTSTEVNFISEMMPFETTEDVMRFHTFVNEVSKENGFGELNAKRLGG
                     SSDASYLTIANVPAICSFGVRGEWNHTSREYAVVDSMFERVKLISTVILNLDKFEV"
     gene            2635..3282
                     /locus_tag="KR250_000692"
     CDS             2635..3282
                     /locus_tag="KR250_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0066657.1"
                     /translation="MDVTAGDNKVTWKGYLSLFVLIVLFSGVFKDSQGFLKALDFANL
                     TGSFGTIADGVDFMGKGGTGAKDGFLFALTLIPTVCFAVGLIDVVESMGGMKAASKIF
                     NPLLRPLLGIPGIAGIAFVSSFTSSDVASIMTKELFESGEITDDERTIFVAYQYAGSA
                     VILNTINTQAPLLPIALLALGPIILIEIFCKILGANLVRLIIGVRNKKNKAKEAV"
     gene            3284..3793
                     /locus_tag="KR250_000693"
     CDS             3284..3793
                     /locus_tag="KR250_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition protein"
                     /protein_id="HBH0066658.1"
                     /translation="MSQQNTGNAIVNSKPKKTAVEIFMGGAKKGFYIGVEQILPAMIM
                     GYVIVQFLQLTGLVDILGNIFGPVMGIFGLPGESVLVLIAAFFAKAAGAATAANLFAQ
                     GLITAPQATILIMPCMLMGTLVGHYARIVLVANASTKYRMLLLAVPIFDAIVGMLIVR
                     VLLTVMGLM"
     gene            complement(4157..5269)
                     /locus_tag="KR250_000694"
     CDS             complement(4157..5269)
                     /locus_tag="KR250_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HBH0066659.1"
                     /translation="MKISEVATPNFLLDLDQLEKNIDKIQQICTNNNKQLWPMLKTHK
                     STYIARLQKNAGASGFLVGTLDEGEALIKAGFEDIMLAYPYMGEVNINRIVTMASKIN
                     LICATDNIECAKAYSEAFSKSKIECKLLIIVDCGLHRFGVEPEKVVELATEISKLENI
                     KIVGISSHPGQVYGCSSPEGVPDVCVQEDSSMEKAYKALTDNGFNIEIVASGSTPTVE
                     SEAASKTITAVRPGNYVYYDAIQIGLGCATEDMCALTVVCTVISKNSSGYYLIDCGSK
                     CLGLDKGAHGNSSIVGYGRVCGHPELTIDSLSEEVGKIKVNGETDIKIGDRIRIIPNH
                     SCSTANLTSNLLGFRGDTIEKVIEVDIRENSKQIIL"
     gene            5567..5833
                     /locus_tag="KR250_000695"
     CDS             5567..5833
                     /locus_tag="KR250_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HBH0066660.1"
                     /translation="MNLEPKKLIEQIGKEKDKKDIWIVGGGDIVKLFMKDNLIDDYYI
                     YILPIILGRGISLFKSGIDKTNLNFKKASNIGELVKLEYSKKQK"
     gene            6078..7805
                     /locus_tag="KR250_000696"
     CDS             6078..7805
                     /locus_tag="KR250_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003496244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0066661.1"
                     /translation="MKLILSYLKNYKLLIVLNILAIFSFALVELGIPTIIAKIIDNGI
                     ANQNIAYIKQMGIVIVIISIIGVVGSILLGYCSAKVSTSVTRDIRNDIFEKSQEFSHT
                     EYNKFGISSMITRTTNDAFQIQQFVNILLRTALLTPVMFIISIIMTIRTSVELSIVLA
                     ISVPFIIIGVAIIAKVSQAISSKQQKGLDKLNLISRENLTGIRVIRAFGNDDYETERF
                     EKTNTYYANVSKKLFKLMSITQPAFFLLLNIAVLAVFWISSEKINIGELQVGQLVAFL
                     EYLFHAMFSIMLFSMVFIMYPRAEVSANRIKELLNEEPLIKNPENGIKDTENKGIIEF
                     DNVTFTYPDGEASVLKDISFTAKTGETVAFIGSTGSGKSTLINLIPRFYDVTEGSIKI
                     NGVDIREYDLKALRKKIGFIPQKSLLFTGSIANNIRFGKHKAGESELEYSAKVAQAYE
                     FISKKPRKFDELISEGGANVSGGQKQRLSIARAIIRRPEIYIFDDSFSALDFKTDAIL
                     RAKLKKETKDAIVLIVAQRISSIIDADKIIVLNEGQVVGMGTHKELLKNCEIYYEIAT
                     SQLKKEELE"
     gene            7805..9595
                     /locus_tag="KR250_000697"
     CDS             7805..9595
                     /locus_tag="KR250_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0066662.1"
                     /translation="MKKSKGFKKTIGRLLPFVKKYKFSFIIAIICIISAATMNALAPK
                     TEGLIITQLTKDVISIAKGVPGASVNFDYVTKILVILACIYFANAIFTYTSSFLLTNA
                     IQNTMRDLRNEVENKIRRLPISYFDSNSFGDVLSRISNDVDTISNALQQSFMQIVNSI
                     LVIILALSMMFTINIYMALIALFIIPISYFVSKFVVKKSQSRFSLQQNALGKLNGKVQ
                     EMYTGFNEIKLYGKEEDSIKEFKKVNQELCENGFKAQFISSMMNPMVSLVTYFGIAAV
                     AVVGSIYAVSGGITVGNLQAFVRYIWQINQPLSQMTQLSTVIQSSFAAIERVFEILDE
                     EEEIPDVENPVKIENVKGNVTFEHVNFGYGENSTLIEDLNAEVKSGQMVAIVGPTGAG
                     KTTLINLLMRFYDVKKGAIKIDGVDIRDMKRKDLRSMFGMVLQDTWLFNGTIFENIEY
                     GRFGATKEEIIQAAKVANVHHFITTLPDGYNMFLNEEASNISLGEKQLLTIARAFISD
                     PSILILDEATSSVDTRLELMLQKAMRNLMNGRTSFVIAHRLSTIRNADLILVMNNGSI
                     IEQGNHDELMEKGGFYEKLYNSQFADKESE"
     gene            complement(9757..10065)
                     /locus_tag="KR250_000698"
     CDS             complement(9757..10065)
                     /locus_tag="KR250_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0066663.1"
                     /translation="MFNKIYEKKEFIVFQVKKGYVVYNTRKSFEEGHTHLKHFEAAKT
                     AIDLAINKKIPRSKDGYYLTSLIRISDDGYYIDKLSELLYVREQKGKKEKYCNSGYQM
                     "
     gene            complement(10327..10875)
                     /locus_tag="KR250_000699"
     CDS             complement(10327..10875)
                     /locus_tag="KR250_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0066664.1"
                     /translation="MIFLNEDVVTNETIDKDYAIEEVRMACKHFGDLYFYFSKVLFEE
                     FGEDKTSEILRKVLFERSEERAIAMRERAHENGDELIADNIISTTDVPFLGWVPEFEE
                     LHCPYGASWLLRFEENPWFKKFASLYCDVTDTTVAEVFTGDTSHKITKNILWGDKSCE
                     RIYFHDDKVSEGKYTYGNKNCK"
     gene            complement(10978..11649)
                     /locus_tag="KR250_000700"
     CDS             complement(10978..11649)
                     /locus_tag="KR250_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBH0066665.1"
                     /translation="MATFKRLQEQAYDYLKELILSGEMIENEIYSETKLASEIGISRT
                     PIRDALQRLSQDGFIDIIPSKGFRIHQITANEIVEIFQIRSAIEGFCTFLITSQYKEA
                     RAVETISKLKHLLDKQKDILLGDKNLNSFAEYDTLFHTTIVSYAQNTEFDKMFNNYMY
                     RIKKLALDSLSHEGRLETTLKEHTDIFNNIANGCIEDIYRTTLIHMETPKYINLEDFC
                     NNKLF"
     gene            12045..12950
                     /gene="garR"
                     /locus_tag="KR250_000701"
     CDS             12045..12950
                     /gene="garR"
                     /locus_tag="KR250_000701"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HBH0066666.1"
                     /translation="MEGYKMKLGFIGLGIMGKPMAKNLLKDGCNLLVYDINKSAVDEL
                     ISCGAKYASVLEMGQECDIVFTILPNGTIVQDILFGMDGLAKTLKEGSIVVDMSSVTP
                     TESILCANKLKDMGLEFIDSPVSGGEPKAIDGTLAFMAGGKEEIYKKVEPFFNIMGSS
                     SILIGDNGSGSVTKLTNQVIVNLTIAAVSEAFVLAAKAGADPEKVYKAIRGGLAGSTI
                     LDAKIPMIMNRDFKPGGKISINLKDIKNVMQTAHNLDVPLPMTSQLLEIMQTLKVHGH
                     LEDDHSGIAQYFEKLAGVEIKKHNN"
     gene            13086..14468
                     /locus_tag="KR250_000702"
     CDS             13086..14468
                     /locus_tag="KR250_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HBH0066667.1"
                     /translation="MITGYGLLIAFVISIGILLVSIIKFKVNPFLALLITSIITGFMV
                     RMPINEISTTISTGFGNTLGSIGIVIGLGIIFGNILSESRATESIAKGLLAKTGEKNS
                     ALAVTTAGFLISIPVFMDAAFVIMMPIIKYVSRVTKKSLMVFVCALGVGTIVGHALVI
                     PTPGPLAVAANVNANVGSFILYSIIVAFPAALIGGWIYGKRFEKYPAYAIDENDREKN
                     LEQGKDSIKIEDDDSSKVPGFGISMFSLLFPILLILISNVFSMFLEKGSTMSGVLAFI
                     GDKNIAILLGILVAIGFLKKYINKPMGDVVIEAADSAGLILLITGSGGAFGSVINASG
                     IGNFLVDTMSGLSISVVVLGFLLSALLRISQGSATVALVTTSSILGPTILATGMSPVL
                     VGLAICAGGVGFSLPNDSGFWVLSRFSGLSVKDTLNSWTIGGTIAGVVAFIMVLLLSV
                     INGIIPLPGL"
     gene            14539..15975
                     /locus_tag="KR250_000703"
     CDS             14539..15975
                     /locus_tag="KR250_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HBH0066668.1"
                     /translation="MREVKPISIDILNTFKQVDEDRLNKLLADELKHLDRKIVVLDDD
                     PTGVQTVHDISVYTDWDKDSMEQGFNEKNSMFFILTNSRGFTVAQTTKAHKEISKNIV
                     DVSKKVNKDFIIISRSDSTMRGHYPLETNLLKSEVERLSEKLFQGEIIMPFFKEGGRF
                     TIDNVHYVKEGEILVPAGMTEFAKDKSFGYKSSDIGEWCEEKTNGKYKSSDMIYISSK
                     ELRELNIEAITEKLKKAEGFNKIIVNATDYVDVKVFTIAFIRAVNSGKEFMFRSAAAI
                     TKILGGVSDKELLTKDELVSKGNTNGGIILVGSHVNKTTQQLEELKNCKYPIEFIEFN
                     QHLVLQENGLKNEVKRVIKVVEEKISRGKTVAVYTRRERFDLDTNDKDKQLMISVEIS
                     DAVTSIIGMLNVRPNFIIAKGGITSSDVGTKALRVKKATVMGQIKPGIPVWMTGKESK
                     FPNMPYIIFPGNVGEISTLRESVEILMG"
     gene            complement(16161..17015)
                     /locus_tag="KR250_000704"
     CDS             complement(16161..17015)
                     /locus_tag="KR250_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0066669.1"
                     /translation="MSTFYIQFLYFVIYSFIGWCCETTYCSVLQKQFVNRGFLNGPFC
                     PIYGFGALAIVATLTPFVHTIPLLFLFSIIITSIMEYCTSFILEKIFNMTWWDYSKHK
                     FNIHGRVCLENSLMFGVLSLIVMLIVHPIVVDFINSISKNVLFIFAISIEIYFVLDLV
                     ITVHTILQLNGKLKQINLIIKELKDKKEYYKLITQETIENKLESLVENRLDTLEDRFE
                     ILKEKENYRYAKNRIDELKNKLNTLLSNHKLLHRRIIKAFPNISSNKHTDILNKIKEN
                     IKSIKKDK"
     gene            complement(17283..17606)
                     /locus_tag="KR250_000705"
     CDS             complement(17283..17606)
                     /locus_tag="KR250_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HBH0066670.1"
                     /translation="MERINCNVINCSHNNSHICYADKIFINGEKAHNDRHTCCSSFLD
                     ESIYSNLSSNVNNRNCEELVCTVKNCKYNKDGSLCTLDNILVAPETDRVNLYIETCCS
                     SFECK"
     gene            17809..18438
                     /locus_tag="KR250_000706"
     CDS             17809..18438
                     /locus_tag="KR250_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0066671.1"
                     /translation="MFEKKKKELFPWKISALILVGVFVLSSGVYIGTRLKHNGVQTGE
                     VVSTSANMNEKSKEAFGLNKNCEVWLQSEGENETNYKRTLTMAGIVPESLLDKTKSEI
                     VSYFKKEYPTKKIKSMDKNEIILVESKYSDNKDVQAMDVTIKGKYTIENDNGNIALYK
                     YDDSGSKTLVEKTKIRVDSLPKTVQDEIKKGVVMDTEEEAYSRLEDFAS"
     gene            18781..19290
                     /gene="ruvC"
                     /locus_tag="KR250_000707"
     CDS             18781..19290
                     /gene="ruvC"
                     /locus_tag="KR250_000707"
                     /EC_number="3.1.22.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HBH0066672.1"
                     /translation="MIILGIDPGIAIVGYGIIEYKNSKFKVIDYGAVTTPAHMNISRR
                     LELVYKGIDTIVKNYNIDEVGMEELFFNKNVKTAITVAQARGVTMLACAHNGKPVYEY
                     TPLQVKQGVVGYGRADKAQVQQMVTSFLSLKKVPKPDDVADALAVAICHAHSNKLEKT
                     LKNIGGKYV"
     gene            19283..19885
                     /gene="ruvA"
                     /locus_tag="KR250_000708"
     CDS             19283..19885
                     /gene="ruvA"
                     /locus_tag="KR250_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HBH0066673.1"
                     /translation="MYSYIKGTVEEIYIDSIVVENNGIGYKINVSSNTIMNLQVGEAT
                     KIYTKLIVREDDMSLCGFVSREELKMFELLTSVSKIGPKVALSILSFASPAQLGAYIL
                     SEDIGKLSKAPGVGKKTAERIVLELKDKVDKNNIEFEPTLLSQKPTLISQDESVDALV
                     ALGYTLSESKEAVQKCKKDGMNTEAIIKKALTYIMSKSLK"
     gene            19992..21011
                     /gene="ruvB"
                     /locus_tag="KR250_000709"
     CDS             19992..21011
                     /gene="ruvB"
                     /locus_tag="KR250_000709"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HBH0066674.1"
                     /translation="MQGFEDENRIITSTMKMEDIDIENSLRPKTLEDYLGQEKSKEQL
                     SIFIEAAKSRNEQLDHVLLYGPPGLGKTTLASIIANEMGVNLRITSGPAIERAGDLAA
                     ILTNLNENDVLFIDEIHRINRSVEEVLYPAMEDFCLDIIIGKGPSARSIRLDLPKFTL
                     IGATTRAGMLTNPLRDRFGVICKLDYYTVDELSKIVLRSSSILDAEIQSNGALELAKR
                     SRGTPRIANRLLKRVRDFAQVRADGKITDKVAKDALELLGVDSLGLDFVDEKLLMTII
                     EKFRGGPVGLDTLAASIGEDRNTIEDVYEPYLLQLGFINRGPRGRVAMPLAYEHLKIP
                     YPNEK"
     gene            21329..22354
                     /gene="queA"
                     /locus_tag="KR250_000710"
     CDS             21329..22354
                     /gene="queA"
                     /locus_tag="KR250_000710"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HBH0066675.1"
                     /translation="MKTSDFKFDLPQELIAQVPIEDRASSRLMVLDKETGNIEHKVFR
                     DIIEYLNPGDCLVLNNTRVIPARLIGEKLETGGKIEFLLLKRTEEDTWQALVKPGKRA
                     KVGTKFSFGNGKLIGEVVDLSDEGSRIIKFHYDGIFEEILDELGNMPLPPYITARLDE
                     KERYQTVYSKHNGSAAAPTAGLHFTEELLNKIKEKGVDIAFVTLHVGLGTFRPVKVED
                     VLNHKMHSEYYMVSQEAADKINRAKENGKNVICVGTTSCRTIESACNEYGKMKETSGW
                     TEIFIYPGYKFKVLDKLITNFHLPESTLIMLVSAICGKDNVLNAYNEAVKERYRFFSF
                     GDAMIIK"
     gene            22366..22851
                     /locus_tag="KR250_000711"
     CDS             22366..22851
                     /locus_tag="KR250_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0066676.1"
                     /translation="MHSLQKQKKNNPILKIAFIFERVLAVVVLIAVFLGTIDVLRLMW
                     GAYIVDFQNPVQYSQLNDFLAQILLLVIGVELVVMLSLHIPGAFIEALLYAIARKMLL
                     LPKNEGMIDVLIGVIAIAGLFAIKKFLVTKDKSALNIMSIHDEEAIKEQQNKEDEDKS
                     I"
     gene            22930..24051
                     /gene="tgt"
                     /locus_tag="KR250_000712"
     CDS             22930..24051
                     /gene="tgt"
                     /locus_tag="KR250_000712"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HBH0066677.1"
                     /translation="MYAVRYELIKTCKQSGARLGRLHTPHGIIETPIFMPVGTQATVK
                     SMTPEELKEIGSQIILSNTYHLYMRPGHELIKRAGGLHKFMNWDKPILTDSGGFQVFS
                     LGPLRKIKEEGVEFRSHLDGSKHFLTPEKAMEIQNALGSDIMMAFDECAPYPSDREYV
                     KNSLERTTRWLKRCKDAHNNTDKQALFGIIQGGMYKDLREQSAKEITNIDLPGYAIGG
                     LSVGEPKPLMYDVLEHTTPLMPKDKPRYLMGVGSPDDLVEGVIRGVDMFDCVLPTRIA
                     RNGTAMTSQGKVVVRNATYAEDFTPLDPECDCYACKNYSRAYIRHLIKANEILGARLI
                     TTHNLHFLLNLMKQIRQAIMEDRLLDFRNEFFAKYGYEI"
     gene            24185..24478
                     /gene="yajC"
                     /locus_tag="KR250_000713"
     CDS             24185..24478
                     /gene="yajC"
                     /locus_tag="KR250_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HBH0066678.1"
                     /translation="MPQQQIIMSIGLWVVVIAIFYFLMIRPQKKKDKQLKEMRSSLSV
                     GDKVITIGGIVANVAKVEDDVVILELGPNRTKVPFEKWAIGTVKSKKEEIEED"
     gene            24536..24898
                     /locus_tag="KR250_000714"
     CDS             24536..24898
                     /locus_tag="KR250_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04086 family membrane protein"
                     /protein_id="HBH0066679.1"
                     /translation="MGKSNYIFKGLGYAYIITLAVLLVYNLFLTFTDIGGDNITMVSS
                     FITTISAAIGGFYTSKHMKEKGLMYGLLVGLLYIVCIFLTVFLAQEKFVFEIGMIYKL
                     LLISAAGGIGGVLGVNFK"
     gene            24964..25107
                     /gene="scfA"
                     /locus_tag="KR250_000715"
     CDS             24964..25107
                     /gene="scfA"
                     /locus_tag="KR250_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="six-cysteine ranthipeptide SCIFF"
                     /protein_id="HBH0066680.1"
                     /translation="MENKHVKTLSQATLKQSAAKGGCGECQTSCQSACKTSCTVANQE
                     CER"
     gene            25734..27629
                     /locus_tag="KR250_000716"
     CDS             25734..27629
                     /locus_tag="KR250_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp8"
                     /protein_id="HBH0066681.1"
                     /translation="MLSNKKRSMAIVMAGATVMSAAAPIFADNTVTENVDKNYTVSAK
                     DSAKLIEEVRKALEVKFEDTKAGANVNDRVYDIKVDNVNLTNATQLQNKINSLTEGQS
                     LKVTIQDKGHQVLGGKVVDYKIENYKTAQEIVDAVNAYNATLAEDSDNKLTATIKSTN
                     TVEVKRAKDSANVITLNVGDQHLDFSKVITSEEGTFEGYEKSYSDIDSKELHTITVKN
                     ADLQDISAEELFDGIRLTTLGREIVNKVKNGYALTFENEAILTQEQEDSDDKDKPEKS
                     SFDIVLSKANEKPETISVSSKNHKLVRDLHKVLTDVKDGKELKVEVLSGDSRFTTAVE
                     VSKERFKDGEAEAIILVGEDAIVDGLASAPLASQKNAPILLSKKDSLPSEIEAEILRV
                     LGSNLSSKKIYIVGGESKVSKETEEKLSKLGVSKVERVSGEDRFETSLEIAKQLKDTF
                     KTAFVVGGNGEADAMSISARAAQFGAPIIVTGNELDANAEKLLKGKELEIVGGENSVS
                     KEVEDKLVDIDLNNKVERLAGENRKDTNAKVINKYYAGATKAYVAKDGYVGGNGQLVD
                     ALTAAPLAASSKAPIVLTTEELSKSQEEVVELRLKNATKLVQIGEGIAKNAIEKIAEK
                     INLFTKN"
     gene            28091..29494
                     /locus_tag="KR250_000717"
     CDS             28091..29494
                     /locus_tag="KR250_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp9"
                     /protein_id="HBH0066682.1"
                     /translation="MKNSKKILAIGLTLFLVMVNTPMVNALTSVEQIKGNDRYETAAK
                     IADKQNYNTAILINSDNSLADGLSASGLAGALNAPILMTKQNQIPNTTMERLNKAKTV
                     YIIGSESTISKNVENQLLSKKKVVQRIFGENRFDTSIKIAEKIKEIKPIDKVIIANGF
                     TGEADAISASPVAARDGVPIILTDGNSVGFDTTGLKSYALGSSEIISDELVKSTNSIR
                     LGGTDRFETNKIVIQEFYKNSKEFYLSKGLQLTDALAASTIAKNAPVVLVENGSNKSI
                     LSGADKLTVLGGINQNVIKQCINQASPNQQGLYYNPNDRAFKERIKGKVYALTKQYRK
                     ENGVRALSVASRLEGLANDWSNLMANKKTLSHTINGKNSYSTFLKYLDWSEIKPGYIA
                     VQGENIIKYKIPDKPVYTNRDADDIGNFIFNEWKTNPEEGTNMLHKGYEIMGFGIAIT
                     GDKNLYATHEFYGRYKE"
     regulatory      29666..29751
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            29875..35838
                     /locus_tag="KR250_000718"
     CDS             29875..35838
                     /locus_tag="KR250_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding adhesion protein"
                     /protein_id="HBH0066683.1"
                     /translation="MKKAISCVLAVSMCSAPLNVFAEPILEGKLRAVEKSVTEKLRGS
                     LEVDLNFSLPIKNSESESMTNMQLRLKDDSNNSGIIKLGEKDSGTVNVGTDNIEYTIK
                     KLSASRSEIKNKDENISYYNIVFNNLPVGKYNVEVSGDGFKNKEIKDIDISSYSQRVL
                     LSNRASVDNKNKPLNYELFLMGDVNGDGKVDKSDYNKVLENIDSNKREFDLNRDGKVD
                     IVDLDYVQKNLGQSEDAKSLESIVSTNPIVDTSKVELKGNSDVDINGNVENLFSGENN
                     GVTISSKNDEISEEKPAVMDIALSEALNMEVINIKAPEGTAPAKGIVEVTDKDGNVQQ
                     IPYENKEYMKNLKDEEQQEEVNNEQEEVNQEEVNNKDENINQTDNNSNNKDENNDKQQ
                     ENSDKQEANSNTNQDKNNDKENSSMDKPNSDEDTPKEEGNISKDDNSTTSTYSRSALE
                     TYADVQNSDIVINLGKQVAVKKITIKITATTANDRNLAEISKVEFLNNVYKEIPKPEM
                     NIPKINKISTSTAVGNEKVSFYWNNEVNVSAYELILDKIDSKGKVLSTKKLQTSKNTI
                     EVRDIEAYGLYRVSIQSLNGDWSSGYKDATPENLDGVPENVDSNYKPIQFSPDSILEF
                     QVVPDTKPEPPEGINVKGKFKSLDVSWKNHKQAKDFDLYYKEKGSNGSWIKYNDKLIR
                     GTSSKIEGLKDNTTYEVRMTATNHLGTSGMSKTYIGTTESMDAPTTPNYRLINTPKEN
                     GGFGIPTNHIVDVVYPDGYDKSEYSDKNPFDKFNVVDGDFTTHWTFPTYNAAEGTNRG
                     PIITFDKSYTMDTFMATPRFDVSDKTINDFEVRYWDENGQLHNLGDVPFETKTDPVNN
                     NKYVMVRLKEPITATKIQVSVCSSYSYKSNISELKFYEYDSIEDDVRNLFMDGLQVEL
                     KEDVTQEKITELKDRLNTPDKASGEYHPYKTVIERELKLAQDLYNDRATLTDVTTVNQ
                     EINKLGKYKDKNGQYTVNSNNLGMQNDWQALGVAARAGDEITVYVGSKSGKTPKLIYT
                     QYYGESGAYKSGEINLNTGKNVIQLSKLHSLDIECGGSLYIRYAEDTPSGGDIKVRVA
                     GATKIPHLNLNGTINDKSPAGVSESKKKIKAYIEELAKYKDAVKGEPYYPGTNVGNSY
                     GYSEKTGVLNTTDIESDKVTLNVPATAVYQEISKGNSDLNTQVDRLYNSMLAWEQIMD
                     LVYSERGVFKTQDLDGNGTVDDNEFNLTKNDMAPKSRMNIKYQRMFIGAFMYASGLHV
                     GVEYGSVPGLMNGVPFQIDDSGKATGGNLFGWGIGHEIGHVTDIGKMTYSETSNNVLA
                     LLAQTFDDKTHSRLEGSTMDKIYEHVTSNSLGIPSNVFERLGMLWQLHLAYDDDFTGS
                     MLKNNSDADLSNDTFYAKISRKYRALSSSDPINSLPKDQMLVAMASSVVEKDLRDYFK
                     AWGVEITPELNSIMDSKNYEKESRKIQYVNDEARRKILNENIASMAKDTKVSASFSDG
                     IQSGSLVKSNKISIDLDVDKDKDKILGYEIIRSDGNYLDGENGTQVKYRTVGFVDAKG
                     NSKTKFTDNISPLNNRAFTYKVVAYDYHLNPTEEFEVGTVKLSDEGKINKSAWSFITN
                     TVSDGDVRTENDSHGPLQNPEIDNIKDNDASTTYKGKIISKAEWNKDPQKDPDINVEE
                     NPYIIVDMKETLPIVGLKYTKPETEARKFSLKGLFNFRKNTSTTYNPLTNYKIQVSND
                     KKNWEDVSQGTFEYGQDLVGGGKDQDNEARVTFNKDGKLWTYQARYVKLISNNKSNIE
                     VAELDIIGPPGDNIEIGIINNADETRTNGIGKLAEDYVYQSDDSSTSEDETGKIPAGS
                     IVITGEYSGNPAFNLPLLIDKNNKTISGEALLFAEVPEKGELGEISKGTWVYYMPKEN
                     FDSLSDKVKAELYRYNDLQGDTPVGQRFVSDTMYVPVGAKNYDELKTISLTDSNSKAR
                     KATRNSIDISNKKVITVSNKVKGNIVRDK"
     gene            36042..37922
                     /locus_tag="KR250_000719"
     CDS             36042..37922
                     /locus_tag="KR250_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp10"
                     /protein_id="HBH0066684.1"
                     /translation="MSKRKSFIRTIAVSTMAVAVTGSATCAYAAPVLQGTKTYEKVNT
                     IDISVDSVENIVYSFQASIKVQGEVEVVDNEQKEKITWSDNIKSQIKSGNANATCRVE
                     YNKSSNTTTLDIYVTSNEDLLDGNRLNIGRISVKKSGSNSNADYKVLGKGTNDKPALK
                     IVTYNNKTVDYENISSDEGLIFTLINESEVKPIGGTGSSKNDPEKYKVEKSEALEYLL
                     NNIRINYSIVSKETQESGSNVILKLGLAQKTTKGRKATINKYVEVTLPKSLEYIVENE
                     LSKPDELPPDNGNGGNNGGGSNSGGSSSGGSSGGGSSSDSTSNVTVKKLKGADRFETA
                     IKISQSGWTKSDTVVIVNGEDKSMVDGLTATPLASVKNSPILLSSNEKLPQKTVEELK
                     RLNPSKVIVIGGNNSMPNSVVEAIKAVNSKISVQRIGGDTRYQTSINIAKEIDKTNNV
                     SKLYIGAGNGEADSLSIASLAGKEKTPIVLTQKDGVDNEAEQFIKSNKVSNIYFIGGV
                     EKISNKAIEQVGKIANKDISNNRVAGQTRQETNAKVIDKFYSQSKLDGVVVANQDKLI
                     DALAVGPLAAKNNSPVILATNTLDKSQESSLKGKNSSKLFEVGGGIASSVIDKIKSLI
                     EK"
     gene            38289..39890
                     /locus_tag="KR250_000720"
     CDS             38289..39890
                     /locus_tag="KR250_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp11"
                     /protein_id="HBH0066685.1"
                     /translation="MKMNKKILSLGLAVSLILVNFKSVNASSVVEKIYGKDRYETAAK
                     IADKQTYETVILVNTEKSLADGLSASGLSGATKAPILFTQQNKIPADTNRCLKNIKKA
                     YIIGTEDTISKSVEKELDSKNIEVKRIGGEDRLKTSYLIAKEIATIKKVDKVLLTNAY
                     SGEADAMSVSSVATRDGAPIILTDGKSVPFDVKNIQSYCIGSEEIMSNPLVKNTNSVR
                     IEGTDRFETNKNVIDYFFNSADGFYVSDGYQLVDAIAAAPLTKNSPMVLVNDGSDKTV
                     LEGAKNITSVGEINEKVIQQCINASNSNGQPPTITVGSTEVYKGEKFDTGKLNIVAKD
                     NTGKVLPIEVDGFIDTNRVGTYILTLKATDEWGKSTGKRVEIKVLDDKSHDYNSPEFK
                     KMVSTEMYNLINSYRKEKGKEPLLVSSRLEGMANAWSKYMMDKKVFAHYIDGKNAPQV
                     FSEFGMRSEENIAYIYIDSKNVQTTQDAKDLAKAIFEVWKKSPEYNANMLSNEFYSTG
                     FGLYILSDGQVHATQEFLNGNEGSL"
     gene            40109..41707
                     /locus_tag="KR250_000721"
     CDS             40109..41707
                     /locus_tag="KR250_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HBH0066686.1"
                     /translation="MKVNKRVLSIGLTISLIMAGAPNINALSSIEKIQGKDRYETSAL
                     IADKQIYDTIILVNTDNSIVDGLSASGLSGVAKAPIMLVQRDKIPTDVEKRLKDVKNA
                     YVIGTEDTIGKSVENQLKNKGIEVKRIGGEDRIKTSYLIAKEISAIKPVNDGDKVFLV
                     NGYTGEADAMSVSSVAARDGVPVILTDGKSIPFKVDGVQCYSLGSEEIMSNELVSKTN
                     SVRIAGKDRFETNKKVIQRFYKGTKKFYVSQGYKLVDAVAGSPLAKDKPIVLVNDGSD
                     KSVLRGADEVTSLGGMDKKVVDQCISSASDKNTMPTITANDVEISVGDKFDNSMLNIV
                     ATDYYGNDLKANIKGNVDINKAGTYVLNISVVDNLGQKSEISVNVKVVVNASTKDSNS
                     YEFKAMVSNEMYDLVNSYRKEKGKKSLRELDSLAGMANAWSKYMEDKKVFAHEIDGKN
                     AAEVFFGFGARSGENIAYLPMNVKSVYTSKDAKEMAESIFDLWKKSSKYNENMLKEEF
                     YSFGFGMHVSSKGEVNATMEFLNS"
     gene            41997..44195
                     /gene="slpA"
                     /locus_tag="KR250_000722"
     CDS             41997..44195
                     /gene="slpA"
                     /locus_tag="KR250_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer protein SlpA"
                     /protein_id="HBH0066687.1"
                     /translation="MNKKNLAMAMAAVTVVGSAAPIFADTTVKEEGYTVVQDKYEKLL
                     KELKAKIKDGTITSVGVEFDGKPITTLAPKADGSDKDAIAEQLETLTKNQLKGLGDGK
                     YVDFKITYGAKAGVPAASLSADDIQKYADQINASEKILVEVAAGSEAGIAKFDSVNNK
                     VIAGDAPLKVKDAVKATVTTNGSNKKVLTISAAAGLSGFSYGTLKDTGASSSDVDAIT
                     LDTTNATITEGDTKVLDFDNSFKFNESTKKVGSLVTPNTTNTPADPGTKTTVRVIKAV
                     EKTIDVSSNSTTKAKDLAKQYVFTDVSDTDPESLSYMLKNINDGKVAVKNSDGDYEVT
                     IFPEGKRLNTLSASSAKTILGDKDTPAKIVLKASTTKKLADYIDDLITYNNSYSNVQT
                     VAGSDRIETAIELSRKYYNSTDKNALYGDPVNNVVLVGSQAIVDGLVASPLAAEKDAP
                     LLLSSKDKLDSSTRAEIKRVMDLNSSTGIKNNKEVFIAGGVNSISKDVENELKDMGLK
                     VTRLSGDDRYETSLAIADEIDINDKAYVVGGTGLADAMSIAPVASQIKDGEATPIVVV
                     DGKSDKLSKEAEDFLDDAQVDIIGGENSVSAKMEDYIDDATGKSPERISGADRQATNA
                     EVIKEYFDKDGVSNYFLAKDGSTKEDQLVDALAAAAVAGNYGSKHNEDGDITTDASPA
                     PIILATDNLSAEQHVAVSKTATTNGAKNLVQVGQGIADSVVSKLKDLLDM"
     gene            44441..46783
                     /gene="secA"
                     /locus_tag="KR250_000723"
     CDS             44441..46783
                     /gene="secA"
                     /locus_tag="KR250_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HBH0066688.1"
                     /translation="MSVLDVILDKTDELEIKKLNNIVDKIDALEDKIQILDDEALKNM
                     TNVFKDKLSKGETLDDILPEAFAVVREVSKRILGMRQYRVQLIGGIVLHQGKIAEMRT
                     GEGKTLVGVAPVYLNALTGKGVHVITVNDYLAQRDKELMQPVYEFLGLTVGVIINGQQ
                     PVERKLQYQCDITYGTNNEYGFDYLRDNMVTQKSEKVQRELNFAIVDEVDSILIDEAR
                     TPLIIAGGGDEGTKLYELANAFIKNIRDEDFEMDRKDKTIALTASGLSKAEKFFGIEN
                     ITHIDNIEIFHHVNQSLRAHKLMNIDVEYVVRDNEILIVDEFTGRIMDGRRFTDGLHQ
                     AIEAKEGVKINDESKTMATVTFQNFFRLYSKLSGMTGTAKTEESEFEATYKINVVQIP
                     TNKPVIRADLHDKVFKTERAKYNAVVEEIIRINKTKQPVLVGTVSIEKSEKLSKLLKK
                     KGVKHEVLNAKQHDKEAEIISKAGKLGSVTIATNMAGRGTDISLGAGDKEEEEKVKEL
                     GGLYVIGTERHETRRIDNQLRGRSGRQGDPGTSRFFVSLEDEVIKLYGGKSIEKILKR
                     MNANEDDALESKALNRAIERAQKGIEGKNFEQRKNVLKYDDTINEQRKVIYDERNKVL
                     DGSDIEEEIQKMVMDIITEAAEKYLKKFRDYHGYFKYLYNTFMPADTLLIPDLDKKNV
                     EEIVNQTYQISKRVYDLKKMMIGIEELAQLEKRVLLQVVDTYWVDHIDAMDQLRQYIG
                     LKSYAQKDPFKEYAIEGYEMFEALNRNIRITTVQYLYKFN"
     gene            47197..49068
                     /locus_tag="KR250_000724"
     CDS             47197..49068
                     /locus_tag="KR250_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HBH0066689.1"
                     /translation="MNRKNLYVIMAAAMVSTSVAPSFAAEATQVKKQTITKKEATELV
                     SKVRELMAQRYTGGSQVGQPIYEIKVGETSSQLKIITNIDELEKLVNALGENKELLVT
                     IADKGHTTNSANEVVAEAIEKYENSADLSAEANSITEKAKTEATGIYGVATVEASYDS
                     TKDKLVITLKDKTDVVTSKTLQIGIGDEKVDLTANPVDIAGTNLDPSAEGFRVSKINK
                     LGVEGAKNIADIQLAEITIKNSDLNTVSPQDLYDGYRLTIRGNTIANSVGKSISDISS
                     KDSETGKYKFTVKYTDTSGKAIELTVESTNDKELKDVKDALNGNTKVNLIAGEDRYAT
                     AVAIAKETEYTDNIVIVNSNKLVDGLAATPLAQSKKAPILLASDNEIPKVTLDYIKEI
                     IKKSPSAKIYIVGGESAVSNTARKQLESVTKNVERLAGDDRHTTSVAVAKAMGSFKEA
                     FVVGAKGEADAMSIAAKAAELKAPIIVNGWNDLTADAIKLMDGKEIGIVGGSNNVPSQ
                     IENQLADIDKDRKVQRVEGETRHDTNAKVIETYYGKLDKLYIAKDGYGNNGMLVDALA
                     AGPLAAGKGPILLAKNDITNSQKNTLDKKLNLGAEVTQIGNGVELTVVQKIGKILGW"
     gene            49177..49884
                     /locus_tag="KR250_000725"
     CDS             49177..49884
                     /locus_tag="KR250_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HBH0066690.1"
                     /translation="MIEATLFFSPHQDDETLSMGSAIIEHVERSDTHVILCTDGSKSI
                     IRKVLDDGGSCSYHIKDVHKYSLSESDFSKNRDEEFKNSCKAMGVKENNIHIENNRAH
                     DGELSKEKARDIMLKYLEKYPDAKVKTVTPYKALGIHEDHRALGEAALELYREGKIKD
                     LRFYVEPYDYNDFKKVNPNVRVWKVLSSQEEKLLNAMDAYKKWNPENGNYAIGYHSVK
                     SHFDELKSNKIQYVHAP"
     gene            49917..52049
                     /locus_tag="KR250_000726"
     CDS             49917..52049
                     /locus_tag="KR250_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HBH0066691.1"
                     /translation="MKISKRIMSLLTATFFIFALSINIADASSKESLVGSGRWETAIK
                     ISQNGWKDATNAILVNDNSIADALSATPFAKLKDAPILLTQSDKLDSRTKAELKRLGV
                     KNVYLIGGMNTLSTNIEKQLKSENMTFERISGNSRYETSLKLAERLDKEKSISDIVVV
                     NGEKGLADAVSVGSAAAQENMPIILSDPNNGIKVANSFISSKNIKKSYIIGGTYSVSS
                     SIEQSLPNVKRLSGNNRNETNAKVLEEFYKNTDLKNAYITKDGMKKQGDLIDSLAVGV
                     LAAKNSSPVVLVGNSLDSSQYDVLNTKSFDNIVQVGGNGNEDAVKDILDLQEETIYRV
                     KTADELDVALRKADANDIITFKPDDNKKITSSFSIETKKAITVELEGLYTQTITVNMP
                     NGELTNKGTLDGTVKIMDIKNGTFINEGDINDIDIYDPNGCKIENKSGGDIWVLTISS
                     SAKDVNIENKGDISKVTNNSTSVIIRNSGNIDTLSGINAPAISGNKPRVDDTDKDEKR
                     ASGIYPTSEPLSPARAGYISVKIFTKPEDTNYKIYYRVVSSRPSALYIGDRIDTSDWT
                     AVPSSVLPFTIKAENGYYIEAVEINSDTKKVSRWGKSDYIDDGFYLKSETAKGLSADI
                     DFDGDYVKLTATGADSGCTVYYRIVSKKPVSMNVGDKVTISDWDEITTNRVRIEANDV
                     KDKYIELVELDDSTNKVTRWGASDKVVE"
     gene            complement(52102..52497)
                     /locus_tag="KR250_000727"
     CDS             complement(52102..52497)
                     /locus_tag="KR250_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HBH0066692.1"
                     /translation="MILKKHRETILYLFFGAFTTLVNIVSYLFFTRVILFNFMVANAL
                     AWILAVLFAYVTNKFFVFESKRIELIFLFKEFLFFVSFRLLSGIIEMIIMYIMIDLFF
                     VNDITVKVFTNIIVIVLNYLFSKMIIFRK"
     gene            52724..55135
                     /locus_tag="KR250_000728"
     CDS             52724..55135
                     /locus_tag="KR250_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding cysteine protease Cwp84"
                     /protein_id="HBH0066693.1"
                     /translation="MRKYKSKNLSKLLALLTVCFLIVSTIPVSAENHKTLDGVETAEY
                     SESYLQYLEDVKNGDTAKYNGVIPFPHEMEGTTLRNKGRSSLPSAYKSSVAYNPMNLG
                     LTTPAKNQGSLNTCWSFSGMSTLEAYLKLKGYGTYDLSEEHLRWWATGGKYGWNLDDM
                     SGSSNVTAIGYLTAWAGPKLEKDIPYNLKSEAQGATKPSNMDTAPTQFNVTDVVRLNK
                     DKETVKNAIMQYGSVTSGYAHYSTYFNKDETAYNCTNKRAPLNHAVAIVGWDDNYSKD
                     NFASDVKPESNGAWLVKSSWGEFNSMKGFFWISYEDKTLLTDTDNYAMKSVSKPDSDK
                     KMYQLEYAGLSKIMSNKVTAANVFDFSRDSEKLDSVMFETDSVGAKYEVYYAPVVNGV
                     PQNNSMTKLASGTVSYSGYINVPTNSYSLPKGKGAIVVVIDNTANPNREKSTLAYETN
                     IDAYYLYEAKANLGESYILQNNKFEDINTYSEFSPCNFVIKAITKTSSGQATSGESLT
                     GADRYETAVKVSQKGWTSSQNAVLVNGDAIVDALTATPFTAAIDSPILLTGKDNLDSK
                     TKAELQRLGTKKVYLIGGENSLSKNVQTQLSNMGISVERISGSDRYKTSISLAQKLNS
                     IKSVSQVAVANGVNGLADAISVGAAAADNNMPIILTNEKSELQGADEFLNSSKITKSY
                     IIGGTATLSSNLESKLSNPTRLAGSNRNETNAKIIDKFYPSSDLKYAFVVKDGSKSQG
                     DLIDGLAVGALGAKTDSPVVLVGNKLDESQKNVLKSKKIETPIRVGGNGNENAFNELN
                     TLLGK"
     gene            55432..57009
                     /locus_tag="KR250_000729"
     CDS             55432..57009
                     /locus_tag="KR250_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp5"
                     /protein_id="HBH0066694.1"
                     /translation="MKAPKTILTILTIALTLSSISIIPSYALTEEKLIGNGRYETAVK
                     ISQKAYSSSPNVVLVNDNSLADALSATPFAKAKGAPILLTESDKLDDRTEKEIKRLGA
                     KDIYLIGGTAVLNKDIENKLKSNGLNVERINGKDRYETSLILANKLKDIKDIKEVAVV
                     NGEKGLSDAVSVGAPAAQNKMPIILSSPKDGVEAFDKFIRDEKVIKAYVIGGTNSVSR
                     AVEKSLPNAERVSGKDRNETNAKVIEKFYTDTNLSNLYVTKDGSKNENQLIDSLAVGV
                     LAAKNESPVVLVGNKLNTKQRDILSTKKLNTITQVGGNGNEEAFDEIKSLQEKTVFEA
                     KTVEELTDMINIASPNDIINFKPKENTVNEAFRMVTNKPITVNIKGDCSKTLTVDMPN
                     GEVNNYATLVNVIVRNIGEGGFNNHDTITILSVRDKNGRVIENTRNSDIDTLMILASA
                     NDTKLINDGYIGKLIDNSSNSDITNNGTIDKKVNQVEDLEAKVDSIEKAIDSISQKVN
                     KIQDILDKLGFLKKFLS"
     gene            57143..58486
                     /locus_tag="KR250_000730"
     CDS             57143..58486
                     /locus_tag="KR250_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HBH0066695.1"
                     /translation="MGIGIECNKKINKSIDKTRIKKNNLNSNSNSNSNSNSNSNSNSN
                     SNSNSNSNSNSNSNSNSNSNSNKISNNAVDSKLMNSRIKSIDIIRGLSIALMIVCNNP
                     GTWMRMYPQLRHAVWHGVTLADFAFPFFVISLGVTIPISINSKLKNNKSTLSIILSIF
                     KRSILLILFGFFLNYLGNPDLDTVRILGVLQRMGLVYFVTSLAYLLLKKLNVGSTATI
                     ITFLCIATFIIVGYYILAKPYGFELEGSLAQLVDLHFFKGHLYKPEFEPDGFLTSIVA
                     ISSGMLGCTMGCVLLKENLGEYKKFFKILVMSIILLIGAFIFNQYFPFNKRLWSSSFV
                     LLMAGSYGILLSIFYFICDIKNKSKIFTPIIALGSSPIFTYMCLEILSHVFWNVPKLT
                     NKVDYPTTLVEWTTYELITPWAGTTWDSLIFSLLYVLFWVIVMSIMYKKKIFIKI"
     gene            58740..60764
                     /locus_tag="KR250_000731"
     CDS             58740..60764
                     /locus_tag="KR250_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBH0066696.1"
                     /translation="MKKTTKLLATGMLSVAMVAPNVALAAENTTANTESNSDININLQ
                     RKSVVLGSKSNASVKFKEKLNADSITLNFMCYDMPLEATLNYNEKTDSYEGVINYNKD
                     PEYLNVWELQSIKINGKDEQKVLNKEDLESMGLNLKDYDVTQEFIISDANSTKAVNEY
                     MRKTSAPVKKLAGATRFETAVEISKQGWKDGSSKVVIVNGELAADGITATPLASTYDA
                     PILLANKDDIPESTKAELKRLNPSDVIIIGDDGSVSQKAVSQIKSAVNVNVTRIGGVD
                     RHETSLLIAKEIDKYHDVNKIYIANGYAGEYDALNISSKAGEDQQPIILANKDSVPQG
                     TYNWLSSQGLEEAYYIGGSQSLSSKIIDQISKIAKNGTSKNRVSGADRHETNANVIKT
                     FYPDKELSAMLVAKSDIIVDSITAGPLAAKLKAPILITPKTYVSAYHNTNLSEKTAET
                     VYQIGDGMKDSVINSIASSLSKHNAPTEPDNSGSAAGKTVVIDPGHGGSDSGATSGLN
                     GGAQEKKYTLNTALATTEYLRSKGINVVMTRDTDKTMALGERTALSNTIKPDLFTSIH
                     YNASNGAGNGVEIYYKVKDKNGGTTKTAASNILKRILEKFNMKNRGIKTRTLDNGKDY
                     LYVLRNNNYPAILVECAFIDNKSDMDKLNTAEKVKTMGTQIGIGIEDTVK"
     gene            60984..61670
                     /locus_tag="KR250_000732"
     CDS             60984..61670
                     /locus_tag="KR250_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HBH0066697.1"
                     /translation="MSGYTNDECEIPKIKSYPGADKEIASEIDYSIVKGTVLFDLYQR
                     IMDLVLSIIGLVIGLPLIAIFGILIKIEDKGPITYKQERLGKCGRRFYIYKLRSMRTD
                     AEKFGAQWAEKDDPRITKVGKFIRKTRIDEIPQLFNILKGDMGLIGPRPERPNFTVQF
                     NEEIPGFINRLAIKPGLTGWAQVNGGYEITPEEKLKEDIYYIKNRSILLDFKILFKTV
                     KVVLTGDGAR"
     gene            61765..62820
                     /locus_tag="KR250_000733"
     CDS             61765..62820
                     /locus_tag="KR250_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp7"
                     /protein_id="HBH0066698.1"
                     /translation="MIKKISTILSLVLLISISSTIGVFADANPKRELIEGSIPEISTE
                     LNKRAFKDSKEVILVNEESIVDSISATPLAYAKNAPIVVTKSKNLGRVTRNYLKELGP
                     EKVTIVGGLKAVSKDAERNIEKMGMKVERIRGKDRYDTSLKIAREMYRTVGFDEAFLL
                     SSTTGLENAISVYSYAAKSGMPIIWAKDEGFEEQIDFLKGKNLKKIYALGDSKEFIAE
                     IDSNLKNIEGIKQINKSSTNVDLIKKFYDEKDIKKIYTARLDFGSRSDVNEYISLGVV
                     SAKENMPILICSDNLSRAQDKFLKDSNINDVVEVGYTVGDYSLFKSIFNLTFLSCIVL
                     ILLLLLITFRALRYESK"
     gene            62935..64491
                     /gene="murJ"
                     /locus_tag="KR250_000734"
     CDS             62935..64491
                     /gene="murJ"
                     /locus_tag="KR250_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HBH0066699.1"
                     /translation="MSKTAKAALWIMAATMFSKVLGFLRELVLANFYGTGMYADVFVL
                     TLNIPGLIIAVIGSAVATTYIPMYFETKKRLGDEGALKFTNNVLNICYIMAIVIAIIG
                     LLFTEQFVTVFAAGFRNDPAKFQAAILFTKIMISGVLFLSGSKIFSSFLQVNDSFVIP
                     GLIGIPYNIIIIAAIALSAGKNVWILPAGALLAMASQLLFQLPFAFKKSYKYKPYINL
                     KDESIKELVNLVLPMLVGVAVGQLNIFVDRLLATTLGDGKLSALNYANRLNEFVMALF
                     VTSIITVIYPKLAKMSGKDNKEGFISTIVKSSNCIILVVLPISIGAIILAEPLVRILF
                     QRGKFDALSTDLTSIALRLYSLGLLACGVRDVLYRAFYSLSDTKTPMINGSIALIINI
                     VLNLILIRPLGHAGIAISTSTSNIITVILLFISLKKKNGYFGGDKIIKTGLKSLVASG
                     VMAVATLLIYNNLYAFMGSGTIKEIISVGAGVLGGASVYTVLIVLFKVEEMDLAFEFL
                     RKGKQKLLRR"
     gene            64537..66243
                     /locus_tag="KR250_000735"
     CDS             64537..66243
                     /locus_tag="KR250_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HBH0066700.1"
                     /translation="MDYKNNYEMWLNSPYFDEQTKNELLSIKDDEKEIQDRFYKNLEF
                     GTGGLRGIIGAGTNRINIYTVRRATLGVLNYIMKTQGEEGKQKGIVIAHDSRYMSREF
                     CIEVAKTLSAYGVKAYIFEELKPTPELSFAVRYLKCAMGIVITASHNPKEYNGYKVYD
                     SDGGQICIDMANDIIAEVNKIDDYSTIKSIDFEEALSKNLITILDNEVDDEFIKAVKK
                     QVLRQNIIDEYGKKLKIIYTPIHGTGNKPVRKVLNECGFENVMVVKEQELPDSNFSTV
                     KYPNPEEKSVFNIAIEMAKNNGTDLIIGTDPDCDRVGIVVKDSSGEYVVLNGNQVGSL
                     LVRYILESLVEENKLPKNNPTIIKTIVTSELGAKIAKAYNVDCLNTLTGFKFIGEKIK
                     AFEESNDRSFIMGYEESYGYLIGTHARDKDGVVSSLMICEMAAYYSSKGMNLYEALID
                     TYNKFGYYKEDLKSVTLKGIDGIKKIKEMMLYFRSVKIDNVADVKVDKILDYKDGVDD
                     LPKSDVLKFLLEDGSWIAIRPSGTEPKIKFYFGANSDNQEDVEFKLNNLISYILNVVD
                     SI"
     gene            66265..67338
                     /locus_tag="KR250_000736"
     CDS             66265..67338
                     /locus_tag="KR250_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HBH0066701.1"
                     /translation="MKVYNVIMAGGGGTRFWPLSRQEVPKQLINLSGEDALINETINR
                     IDSLAKKDDLFIVTNEKQLEALKDIVKDKCLDSNILPEPCARNTAAAIGFAAFNIMKK
                     YGDGVMCVYPADHYIKDEKEFKSILEKAIYIAENNDKLVTIGITPTFPSTGYGYINFN
                     RENTIEDVAYEVVEFVEKPNYEIAKEYVNSKKYVWNSGMFVWKVSKILEDFKRYLPKV
                     YEKLEDISKYLGTKEEMEKIKEIYPTIQSISIDYGIMERSNDVIVVPGDFGWNDVGSW
                     DSLGAIYPTDDEGNIKRGENITIDTKNSIIYSDDKLISTIGISDLIVVSTNDAVMVCR
                     KDKAQDVKKIVEQLKEEDRQEYM"
     gene            67350..68072
                     /locus_tag="KR250_000737"
     CDS             67350..68072
                     /locus_tag="KR250_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBH0066702.1"
                     /translation="MIPKKIHYVWFGGPKGNIENICINSWKEKLPEYEIVEWNEKNFD
                     IEKEIKGNKFLEECYKRKLWAFISDYTRIKVLYEQGGVYMDTDMQILKDITPLLENNR
                     LVCGYEDDREYINGAIIGVEKGHPFLKDLLEYYEKEVLTSSLFTIPKIMTHLMEKNYK
                     KIDPNNYEEGIRVYDKEYFYPFGFKEDFTPECITENTFGIHWWGKSWAKKRNYFLESK
                     HLTGVNKIWKCCKIFASNTLRS"
     gene            68088..69245
                     /locus_tag="KR250_000738"
     CDS             68088..69245
                     /locus_tag="KR250_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide polymerase"
                     /protein_id="HBH0066703.1"
                     /translation="MKTLNDDRNIMIRKYLYIFVLFIILLRKYMISFIDPNMDIGMIK
                     SALFYSSIGILMLLFLFDKRKSITEMVLVGVCVLLYLLNREGAILLIVLLAVSAKQID
                     DKFIVKNYLIISACFLMVAILLFNLFPSLIFNQEVHYRYIEKIDMLVTRMDFGLGNPN
                     SVFYHMVTIYAAYIFLRYKDYNKWDRILLFGSAFFVYQTTYSRTGFFTILAGLIFVEI
                     IRWIDIKKIKGLPMLLKTLPVILTLFSVIIGTVFDKSTLLNRLLASRPKFWNVYLAEK
                     GNFLKPFGNSYSPAIKATNPLDSSYVYIISVLGVVACILFMYLMYKGIESFIEKDKKA
                     YLVALFIFLLYSFAENLLLEASFSFAVVLLIKEVILNDKREIDLWKMKSRR"
     gene            69248..70087
                     /locus_tag="KR250_000739"
     CDS             69248..70087
                     /locus_tag="KR250_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBH0066704.1"
                     /translation="MLISLIMPTLNRYDDIYLLMDSLENQTYKNFELIVVDQNDNSKV
                     KEIVDKYIDKLDIKYIKSSKKGLSYNRNVGIDNAVGQIIGFPDDDCVYENDTLEKVIN
                     FFNKNKDYKIYSCKTMDSNKVDAFKKMYDGTCDITSSNVLDTITSITFFIDFEGKDYT
                     RFDEKLGVGGEFGAGEEIDYVLNLLSLGFKGKYFGNDIIYHPAKKHSKSKEKYQKDYN
                     YGRGFGALCKKEIVYRKNYKFAKVMVSKLVRNIGGLILSSNRDYHSATIKGRINGFRQ
                     YKL"
     gene            70102..71277
                     /locus_tag="KR250_000740"
     CDS             70102..71277
                     /locus_tag="KR250_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HBH0066705.1"
                     /translation="MDKVKFLINMLLAFFIYPFNKHKFNGRDIWLIGGHSGDIYNDNS
                     KFFYEYMLKEHNDVETYWVVNKDSKVFDKIPGKKLIRGSVENYLYYYNSKAIVFSHAP
                     SADIAPYNFAVPVLNYFHKKTIKVFLNHGTISFKKRKPMNKKFKNIIDNLYKSYNIVT
                     ASSEFERNVMVNDWGMLDDSVYIIGNARYDNLPTNEVAQTRDILYTPTWRDWIKFSSG
                     KFTDTDYFKNIMNFLNDDKLNKILDEKDINVKIYMHHLMHEFIDDIKENITGKRIVFL
                     DKGVTLANEIRKSAANITDYSSVAIDFLYMNRPILFYQFDLDEYMEKVDSYIDLKSEM
                     FGSLAYNNDEAVNKLIDIIENNFEVMDNQKNERNKFFRYNDNKNCKRIYDCVLSKIK"
     gene            71306..72055
                     /locus_tag="KR250_000741"
     CDS             71306..72055
                     /locus_tag="KR250_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBH0066706.1"
                     /translation="MKKNLVSIITPMYNSEKFIEATIKSVLNQTYQEWEMLIIDDCST
                     DNSPNIVKSYMQQDSRIKCIKTETNKGVSNARNLALSKATGQFIAFLDSDDQWNSSKL
                     EKQVNFMLENDYVISFTSYELMDENDKKLNKVIKVPPNVDYRRLLKGNILGCLTVVID
                     KSKLDFEIRMSGVRHEDYVLWLSILKKGHIAHGINEVLALYRKSSNSLSGNKIKAAMW
                     TWNIYRNIEKIPLYKAIYYFINYGINGIKKS"
     gene            72077..72985
                     /locus_tag="KR250_000742"
     CDS             72077..72985
                     /locus_tag="KR250_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBH0066707.1"
                     /translation="MYKYKFTVFTPTYNRAHLLENLYNDLKAQTYDFNDFEWLIVDDG
                     SSDSTKELVEKFISENKLNIRYIYKENGGKHTAINLGVKNADGELFFIVDSDDGLIKD
                     SLEIANDEWDSLENKEGFSGVVGLCVYPSGDLIGTEMPEDKKICHFADLYFKYGVKGD
                     KSIVFVTDELIKFPFPERKEVRFLPESVVWNEMSKYYKVICVNKPMIIRDYLDDGLTK
                     NILSKNALRGRALEFLYLINQNTYPLSRYPYMWIKNYINLARYSLLSDSHYFGELRKV
                     SDKLLYLALFPLGYYKYIGQRKLVSK"
     gene            73033..73785
                     /locus_tag="KR250_000743"
     CDS             73033..73785
                     /locus_tag="KR250_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBH0066708.1"
                     /translation="MNEPLVSIITPVYNSEEFLSETIKSIQNQTYKNWQLLLVDDCSK
                     DNSSEIIKSFRKEDARIKYIKLEKNSGAAVSRNVGIKNAEGRFIAFVDSDDLWDSRKL
                     EIQIEYMLKENVGFSFTSYRYMRQDGSKTNKVARAPKKIDYEGLLRNTIIGCSTVVID
                     KEIVGEFSMPLVRRGQDTATWLQLLKKEKYAYGIQEDLVNYRLVGNSISSNKIKALKR
                     TWNTYRNVENLSLPKSLYVFCFYVFNAIKKRV"
     gene            73763..75106
                     /locus_tag="KR250_000744"
     CDS             73763..75106
                     /locus_tag="KR250_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HBH0066709.1"
                     /translation="MPLKKEYKFYILGGKKMKIAIAGTGYVGLVTGVCLAEVGNENVV
                     CVDVDEQKVKSMKLGIAPIYEEGLEELMVKNYNRGRIDYTTDYKKAYEDVDVILIAVG
                     TPERSDGSANLDYIKVVAKQIAETVTKDTLVVIKSTVPIGTNEEIEDYINRNLKNDVK
                     VELASNPEFLSQGTAVKDTLHASRIVIGVESEWAREILEEIYKPFNQPIIVTNRNSAE
                     MIKYASNNFLALKISFMNDIANLCEIAGANIEDVALGMSYDDRIGSKFLKAGIGYGGS
                     CFPKDTKALHWLSEHNGYELKTVKAAIEVNESQKLKLVRKASKLVESFQDMKVAVLGL
                     TFKPDTDDIREAPSIPNIKYLLERGARVTVYDPIGVNNTKKIFGDSILYADSVDDAIE
                     DAEICFIMTEWKDVLNYDIHKYKEKMKNPLVLDGRNCYKIKDMEELGIEYYSIGR"
     gene            75112..76203
                     /locus_tag="KR250_000745"
     CDS             75112..76203
                     /locus_tag="KR250_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBH0066710.1"
                     /translation="MKTVCYLADGSNPHTLKWCSFFKNKGYDIHVISLNGGHMEGIKI
                     YNFGSNVEELKNENISKKTGYLGSIKQIKKLVNEIKPDILHAQYASSYGFIGSLLGYH
                     PYIISVWGTDIYDFPNNGFIQKQIIKHNFRKADYIFSNSRDMAKEANKYTAKHVDVTF
                     FGVDMDRFKPMEVEKEDAFVIGIIKSLEKKYGIEYLIQAFKMLKDEYKDKKIILKIGG
                     SGSQMDNLINLAKELGIENDVQFLGRISPENVSKTFNSFDVTVFPSLREGFGVAAIES
                     EACEVPVIVTNVGGHPESVWENETGLIVEPKQPEEIKNAIIKLMENDELRLNMGKKGR
                     QFVRENYEVNLNFNDIEKIYDSIFDKYKK"
     gene            76287..78200
                     /locus_tag="KR250_000746"
     CDS             76287..78200
                     /locus_tag="KR250_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBH0066711.1"
                     /translation="MRESIDLEKESFFNRLFKKYKFRHLTLLILDICSVLVAFRMSLS
                     LTGNLNAFTTNDIIISVCIYIVLHIVSFRLFKCYNTLWRYAGEEEIISIFVATLAYLI
                     PIYIINKLLGFDYPIMFYVLNTIFIIMFTSGARIAYRAIRIVMNKTYSRGKVSNILII
                     GAGDAGEMVIQELKRNPELKKVAVAIIDDDKNKIGRIIHNVKIVGTTSDIKDVVEKHN
                     VDEIIFSIANIEKRRKKEIIDVCKNTNCKIKTIPGIYEIIDGKVDIKQIREVEIEDLL
                     GREPIKTNLREISNYIEGKVILITGGGGSIGSELCRQIAGFNPKELIIVDNYENNAYS
                     IQQELLRKYKNKLDLKTVIASIREEKRMDEIFNKYKPEVVFHAAAHKHVPLMESSPGE
                     AIKNNIFGTLNIAGLSSKYRAKKFVLISTDKAVNPTNIMGATKRAAEMIIQTMNAESQ
                     TEFVAVRFGNVLGSNGSVIPLFKKQIEDGGPVTVTHPDIIRYFMTIPEAVGLVIQAGA
                     MAKGGEIFVLDMGEPVRILDLAKNLIKFSGFEPDVDIKIEFSGLRPGEKLYEELLMSE
                     EGLLDTEHKKIFIGRPIDVDREKITKYLKLLREITNNEEVEKIDGIMRELVPTYIKPE
                     DANIKEIATTREK"
     gene            78399..79106
                     /locus_tag="KR250_000747"
     CDS             78399..79106
                     /locus_tag="KR250_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBH0066712.1"
                     /translation="MMNIKNKKHILKKFIAMVLIAGVVTVEAGAITASAAEPTNSPMS
                     ATVDQCDFLNVRSGASANDAVVGKINTGDKVEVLELHSNGWIKIKSVDNVTGWVNGDY
                     LTIQGGNVDAKVQNVLNLAFKQQGKPYRWGATGPNAFDCSGFTSYVYKNGAGVNLPRV
                     SRSQATVGKKVSRAELKPGDLVFFGSGGSINHVGLYVGDSKFIHSPQTGDVVKVTSMA
                     PGTNYARRLITATRVLQ"
     gene            79408..81519
                     /locus_tag="KR250_000748"
     CDS             79408..81519
                     /locus_tag="KR250_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding glycosyl-hydrolase Cwp19"
                     /protein_id="HBH0066713.1"
                     /translation="MKKISILVLSLIMTLTMCSVSSFADSSNDKEMRAAWISTVYNLD
                     WPKTKNNEAKQKKEYTDLLDKLKSVGINTAVVQVRPKSDALYKSNINPWSEYLTGTQG
                     KDPGYDPLPFLIEEAHKRGMEFHAWFNPYRITMADESIDKLPANHPAKKNPSWVVKHG
                     NKYYYDPGLPEVRKYIVDSIAEVVQNYDIDGVHFDDYFYPGVSFNDTATYQKYGKGQN
                     KDDWRRENVNTLLRDVKASIKSIKPNVVFGVSPAGIWRNKSSDPTGSDTSGNESYVGT
                     YADTRAWIKQGLIDYVVPQLYWPIGLKAADYSKLVAWWANEVKGTNVDLYIGQGIYKQ
                     GQSSYGGQNIAKEIVQQVTLNRKYSEIKGSMYFSAKDIANSTSIQKDLKSLYSSSEEP
                     VTPPSNVKVEKLRGDERYDTAVAISKKGWATNSDTVVLVNGYSIVDGITSTPLATSND
                     APILLVNKDNIPTSTKNELKRLNPSKVILIGGNNSIGDKVESEIKDTLSNVSINRVGG
                     SDRYSTSLMIAKELVKTNPVEKLYITSGTGEADSLSIASKAGEEKQPIVLVSKDNVSD
                     EVYNWISDLKVKDAYFIGGNLSISDSVINKLDKVITNDVSKNRIAGENREETNGKVIQ
                     KFYPNAEYSSMFVSKSNQLVDALTSGPLAAKLKSPVVMLGNSVTSAQKTALEPKKTTL
                     VYEAGDGINQNTLNTFLNLVK"
     gene            complement(81717..82682)
                     /locus_tag="KR250_000749"
     CDS             complement(81717..82682)
                     /locus_tag="KR250_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBH0066714.1"
                     /translation="MSKLKKFVILLAFLVVIFPISVYGYFYYKLSAIHDSSISSDLLD
                     NNDHKNEDGIINILLMGTDARPNEDSSRSDAMMILTIDNKHNDIKLTSLARDSYVDIP
                     GHGKQKLTHAYAYGQADLLIQTIEENFNIDIQNYACVNFESFMYIIDAIGGVEVTVEK
                     GEIRELNKFIPETYKWSKSDDKGSIQYIRNSGKQTLNGYQALSFARIRHNDTAFARDG
                     RQRQIIQAIIKKTETLPVTKYPGLLDAVLPYVKTNMKPNAILSLGAQVLKMGDLNIKQ
                     FEFPIDDEIHSTGGIYGKAGWVLRFDPDTLDILHDFIFNDIEFKQ"
     gene            complement(82967..83923)
                     /locus_tag="KR250_000750"
     CDS             complement(82967..83923)
                     /locus_tag="KR250_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBH0066715.1"
                     /translation="MSGLKKFVIFLALLVIAFPVSIYGYVFTKLKTIQDDSINTEALS
                     SNDYKNEENITNILLLGTDARPGEEVSRSDAMMILTIDNKNKSLKLTSLNRDTYVNIP
                     GHGEQKLTHAYVYGQANLLIETIEENFQLDIQNYAVVDFFSFMDIVDALGGVTVTVDK
                     SEIRELNKFIPETYKWNKNDNKGSLKYIRNSGEQKLNGYQTLAFSRIRKNDSTDERDR
                     RQRSVIQSLINGVKDLPVTKYPNLVNTILPYVKTNMNPNEIISLGKELLSIGNLNLKS
                     MEFPLSTENGRKIGNAGYVIPFEEYELDAMHDFIFKDIMVED"
     gene            84291..85079
                     /locus_tag="KR250_000751"
     CDS             84291..85079
                     /locus_tag="KR250_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBH0066716.1"
                     /translation="MIKLIATDLDGTLLDEKSEINPEFYKVFKKLRERGIMFSAASGR
                     QYQNLIKKFEDIKDDMMFISENGTLVVYKGKEILSNPLNKELVNEIIETTRSIKGKKI
                     VMSGKKYAYIESKDEAFIQEVSTYYAKFKIVEDLTKVEGDILKIAVFDFKGAEHNNNI
                     YFEKFSDRAQVCISGVEWLDLTAKGANKGSAIKKVQKMLDIKYEETMVFGDQLNDVEM
                     MKSAYHSYAMENANEHLKQIARFRAKRNTENGVVDKIKEVIKIG"
     gene            complement(85202..85672)
                     /locus_tag="KR250_000752"
     CDS             complement(85202..85672)
                     /locus_tag="KR250_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4358 domain-containing protein"
                     /protein_id="HBH0066717.1"
                     /translation="MKKILILFFSIILSLGMVSCSSSGDYKNVSTSKIKKALKSSDLL
                     IKESKIFDAKDFNYFNDVEEDIIQGFVINSSEKLSLEDIIVIKTDEVDKIYKTLENYK
                     KDMIQAPFGEGHGEKINSEIANNTIIEKAGRYVYLISAENATQIENKILNIIKK"
     gene            85811..86455
                     /gene="uppS"
                     /locus_tag="KR250_000753"
     CDS             85811..86455
                     /gene="uppS"
                     /locus_tag="KR250_000753"
                     /EC_number="2.5.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-trans,poly-cis-decaprenylcistransferase"
                     /protein_id="HBH0066718.1"
                     /translation="MRIPKHVGIIPDGNRRWASQNGMTKDKGYESGIDPGLESYRLCR
                     DLGCEEVTFYGFTTDNTRRPSVQTKAFTKACIDAANILIDEGAALLVVGNTKSPMFPE
                     ELLPYTNRIKAKEGQIKANLLVNYGWYWDLNNISGVENVNKNNIQDYIQSFDVSRLDL
                     IIRWGGRRRLSGFLPVQSIYADFYVIDNYWPDFKSDDIKDAFSWYDTQDVTLGG"
     gene            86498..87403
                     /locus_tag="KR250_000754"
     CDS             86498..87403
                     /locus_tag="KR250_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBH0066719.1"
                     /translation="MKKKLLDGKITLIICKSVKIYTKKGEEMSKGNNNNNSRNKSKKT
                     SHLNRKKRKLNKKKLAVLICFTVLFLFIAFKATQGVVALVKSMDKSNKTSQQQNVNSE
                     QFDLGNEEENKKKKYTVFIDPGHGGNDKGTESKTSNRYEKDLNLQIAKKLANKLSKQK
                     DIQVVVSRTDDTYISLKDRAILANNSSADVLVSIHLNAEKNGNTATGIETWYRNKATD
                     GSKELAQTVQSTIVSYVKVRDRGIVENNFEVLRESNMPAILIECGFLTTPSEEQKIIN
                     EKYQDQLAEGIVQGVLSYLDSKGNK"
BASE COUNT        34801 a         9841 c        15653 g        27245 t
ORIGIN      
        1 ttatttgaat ttctatcatt aatcatacac atgattttag atacatttgt attagataga
       61 ggtataaaag aagcaacttt attggctatt gagctatctt caatattatt agaattttta
      121 tttgaatata aagggcctat tgtatcatta gtatcattta ctataaaagt acagtttcca
      181 ttcaaagaag cagatttacc attagcttga cgagcatttt gctctgcatg aatatttgta
      241 tttccaatgc cccaacctat atttaagcta tatttaattt cttttaaatc ttctgaaata
      301 aaaaaatctg tatagttatt ggtcatattg tttaacattt ctttagtagt gtgtatctct
      361 attttcatat catttttttt gacctgaact ccataaaatc catgtcttga tagagaattt
      421 aatatagtgt tatatatttg attttgtatc tcatcatttt taaatatcaa atcttcattt
      481 atgtactctg aactatttac tgtaactata caagttgcag atttattttt gtctaagttt
      541 tgtaatgtta tttcattcac cagagattta aatgtattca ggattgattc tgtagatgga
      601 accatgtaaa tacaatcata tccaagttta ttaagttcac aactaatgat gtaatttcta
      661 gtaaaagaaa ggttaatctt attatctcgc aataagtcta aatgtatttc caataattta
      721 tcatttaatt ttttaaagtc atatacaggt tgagggatat taaaatcttt aattgtatat
      781 ggacatttat tagagtctat taatgaatat atatcataat aattgttgtc ctctcttaaa
      841 aaatccataa atgattttgt aaagtcaata tctttttggc tatttaataa tttgaataag
      901 tatttataaa aatctctttc atcaagcttt aaaaaattta atggaacttt tatgtctttt
      961 atatttatta taagttcact atatggaact ataccactgc atacaaatcc atcaaaatta
     1021 tctacttgct catagtatat attaggtaaa ttattaaaag gattctcccc tttgagctca
     1081 ataaatgtaa tttcacaagt atcatttaat tctttaagag cttgtttaaa cttatctgac
     1141 ataaaataag atgttgtcaa aaaaccaatt ttatatttca taaaacctcc tttaaaattt
     1201 cgagagtata gtaactaatc aatcaattta taacctcatt ttataatagt agataaaacg
     1261 cgtcaattta ttttacaaaa ggaaaattta aagaaaaatc agaaaatata aaaaagactg
     1321 aaaaatatgt tgactgtcaa gaataattgt gctattttgt aattaaggac tagtctttta
     1381 agatttccta aaataaactt agattttagg aaagaaatac attttatttg aaaataaagg
     1441 agaagattat gaatacatat ttaaatgaaa tttctaagtt tgttgatgag aatagagaag
     1501 aaatagtttc attatggaaa gagattgtta atatagaaag ctatacacat tgtaaagaaa
     1561 gcgtaaataa attggctaag agattaaagt ttgagtttga aaaagaaggt ttagattgcg
     1621 agttagtaga tgttggagat aatggtagta cattgatagg tacacttggt tctaatataa
     1681 ataaaaagcc tataattttt tcaggtcaca tggatactgt ctttgaaaca ggtacatttg
     1741 gagagaatcc atttaaaata atagaaggaa aagcatatgg cccaggtgtt ttagatatga
     1801 agggtggtat aataatttcc ttatatgtta ttaaagcatt aaataaaata ggatacaaag
     1861 aaagaccaat aaaaatagta ttttctggag atgaagagat aggacataag gattcaacag
     1921 gtgcagatgt tatattgagg gaagcaaaag gtggtctttg tgcttttaat atggaaactg
     1981 gattagtaga taatagtttg tgtgtaggta gaaaaggaag aataggatgt aatatacatg
     2041 taaaaggtgt agaaacacac gcaggtaatg attttgaagg tggaagaaat gctatagaag
     2101 aaatggctaa taagatacta cgcatacaaa aattgactaa tttagatgtt ggaactacag
     2161 taagtgtaag tgtgattaag ggtggaagag tggaaaattc tattccagaa gattgtagta
     2221 taaaagtaga ccttagattt gaaaaagtag aagaaatgga gaatgttaaa gaacaagtta
     2281 gagagatatg taaagaaact tacatagaag gaacttctac agaagttaat tttataagtg
     2341 aaatgatgcc atttgaaact acagaagatg ttatgagatt tcatacattt gtaaatgaag
     2401 tgtcaaaaga aaatggattt ggagaattaa atgctaagag acttggaggt agttcagatg
     2461 ctagttattt gactattgca aacgttcccg caatctgttc atttggtgta agaggggaat
     2521 ggaaccacac atcaagagaa tatgcagtag tagattctat gtttgagagg gtgaaattaa
     2581 tttctacagt tattttaaac ttagataaat ttgaggtata aagggggatt ttatatggat
     2641 gtaacagctg gagataataa ggtaacatgg aaagggtatt tatctttatt tgtattgata
     2701 gtattatttt caggagtgtt taaagatagt caaggcttcc ttaaagcatt agattttgct
     2761 aatctgacag gttcatttgg tactattgca gatggtgtag actttatggg taaaggagga
     2821 acgggagcaa aagatggttt cttatttgct ctgactttga ttccaactgt ctgttttgct
     2881 gtaggtctta tagatgtagt tgaatctatg ggaggaatga aagcggcatc aaagatattt
     2941 aatccattgc ttagacctct gcttggtata cctggaatag caggaatagc atttgtatct
     3001 tcatttacta gttcagatgt agcctcaata atgacgaaag aattatttga aagtggggaa
     3061 ataacagatg atgaaagaac tatatttgta gcatatcaat atgctggttc agctgttata
     3121 ctgaacacaa taaatactca agctccactt ttaccaatag cattattggc attaggtcca
     3181 ataatcctta tagagatatt ctgtaaaata cttggtgcta atctagttag attaatcata
     3241 ggtgtaagaa ataaaaagaa taaggctaag gaggctgtgt agtatgagtc aacaaaatac
     3301 tggaaatgca attgtaaata gtaaacctaa gaaaactgct gttgagatat ttatgggggg
     3361 agcaaaaaaa ggtttttaca taggtgttga acagatatta ccagccatga taatgggtta
     3421 tgtaatagtg cagtttttac aattaactgg tttagttgat attttaggaa atatttttgg
     3481 accagtaatg ggtatttttg gactacctgg ggaatctgtt ttagttttga tagcagcatt
     3541 ctttgctaag gcagcaggag cagctacagc tgcaaactta tttgcacaag gtctaatcac
     3601 tgcaccacaa gcaaccatat taattatgcc atgtatgtta atgggtactc tagttggtca
     3661 ttatgccaga atagtgcttg tagcaaatgc cagtactaag tatagaatgt tactgttggc
     3721 agttcctata tttgatgcaa tagtggggat gttaatagta agagttttac tcactgttat
     3781 gggattaatg taaataacat aaaattataa cctaactata aatgatatat aagaattaat
     3841 atatacaagg attaatgagt agtaattgat atatagtaat aaactaagat agtaattaat
     3901 gtgtatatta attaaaaaaa gagagtatct caaagtgaac tttttagttc atgagatact
     3961 ctttttgtat gaaatcaagt atattttcat aatttcaaca atgaaagaga ggcttatctc
     4021 tattttgaga cagcctcttc ttttaaattt atatttctag aatttatgtt ttttatttat
     4081 ccaacattaa cattagttat tagaaataat tttaagaaaa atatactttc atgttaatat
     4141 cagaataaat ctgtttttaa aggatgattt gttttgaatt ttcacgaata tctacttcta
     4201 tgactttttc tattgtatct cctctaaaac ctaataaatt acttgtcaag tttgcagtgg
     4261 aacaagaatg attaggtata attcttattc tatcaccaat ttttatatca gtttcaccat
     4321 ttactttaat ttttccaact tcttctgaaa gtgaatcaat agtaagttct ggatgtccac
     4381 ataccctacc ataaccaaca atactactat ttccatgtgc acctttatca agtccaaggc
     4441 acttacttcc acaatcaatt aaatagtatc cactgctatt ttttgagatt acagtgcaca
     4501 caacagtaag agcacacata tcttcagtag cacaacctaa acctatctgt atagcatcat
     4561 agtatacata gtttccaggt cttacagcag ttatagtttt agaagcagct tcagattcaa
     4621 ctgttggagt actaccagaa gcgacaattt ctatattaaa tccattatca gttagagctt
     4681 tgtatgcttt ctccatagaa gaatcttctt gaacacaaac atctggaaca ccttctggag
     4741 aactacaacc atacacttga cctggatgcg aacttatacc aacaatcttt atgttttcta
     4801 gcttagaaat ttcagtagca agttcaacca ctttctctgg ttcaacccca aaacggtgta
     4861 agccacaatc tacaataata agtagcttgc actctatttt tgatttagaa aaagcttcac
     4921 tataagcctt agcacattct atattatctg tagcacagat taaatttatt tttgatgcca
     4981 tagtcacaat tctatttata tttacttctc ccatgtaagg ataagccaac ataatatctt
     5041 caaaacctgc ttttatcaga gcttcacctt catcaagtgt tccaactaga aaaccactag
     5101 cacctgcatt tttttgaagt ctcgctatgt aagtactttt atgtgtctta agcataggcc
     5161 aaagttgttt attattattt gtacaaattt gttgaatttt atctatattc ttttctaact
     5221 ggtctaaatc tagcaaaaaa tttggagtag cgacttctga aattttcatg ttatttacca
     5281 cctttcaaca aaagtaaatt aactataagt attataatta ttatattagt aatatcgtct
     5341 ttataatatt aatagcttat aattatatta tagaactagc gttaacagag agcaatattt
     5401 atttgtaata attagaattt tcaataagtt agtgtggttt taaattattt atataatata
     5461 tttttaattt tatgcaaagt ttatttaaat tagtttgagg atttattaat agggtattat
     5521 tgaatgagta aaaaagtgat gtttacatag aataatcaca ctataaatta atttagaacc
     5581 taaaaaatta attgagcaaa ttggaaaaga gaaggataaa aaagatattt ggatagttgg
     5641 tggaggcgat atagtaaaat tatttatgaa agataactta attgatgatt attatatata
     5701 tatattgcca ataatattgg ggagagggat ttcactattt aagtctggta tagataagac
     5761 taatttgaat tttaagaaag ctagtaatat tggagaactt gtaaaattgg aatacagtaa
     5821 aaaacaaaaa taaattacat gccaatttgt tacaatgtta tgaaatgaag catttaaaag
     5881 acttttggct atatgaattg acattacttg tcaaatatgt taacatataa gcgacttgtc
     5941 ggagggtgta ttaaaaagaa tatgctggag aagagagtgc gttaactctt ttctcctttt
     6001 tttataaaat taagcattta taaatttagt attattacat gattacacta tctcaaaaat
     6061 taagaaagga gaaaaatatg aaattaattt taagttattt aaagaattat aagttattaa
     6121 tagtattaaa catactggct atatttagtt ttgcattagt cgagttgggg attccaacta
     6181 taatagctaa aataattgac aatggtattg caaatcagaa tatagcttac ataaaacaaa
     6241 tgggaatagt gattgttatt atttctatta taggggttgt aggtagtata ttgttgggtt
     6301 attgttctgc taaggtatca acaagtgtaa ccagagatat aagaaatgac atatttgaga
     6361 aatcacaaga attttcacat actgaataca ataaatttgg aatttcttca atgattacaa
     6421 gaacaacgaa tgatgcattt caaattcaac aatttgttaa tatactacta aggactgcct
     6481 tattaactcc agttatgttc attataagta taataatgac aattagaaca agtgtggaac
     6541 tttctatagt attggctata tcagtacctt ttataattat tggagtagca ataatagcaa
     6601 aagtatctca ggcaatatct tcaaaacaac aaaaaggact agataaatta aaccttatat
     6661 cgagagaaaa tttaactgga ataagagtaa ttagagcttt tggaaatgat gattatgaaa
     6721 cagagcgatt tgaaaaaaca aatacatatt atgctaatgt ttctaagaaa ctatttaagc
     6781 ttatgtcaat tactcaacca gcattctttt tactattaaa tatagctgtt ttggctgtat
     6841 tttggatttc aagtgagaag ataaatattg gagagcttca agttggacag ttggttgcat
     6901 ttttggaata tcttttccat gccatgtttt caattatgct tttttcaatg gtatttatta
     6961 tgtatccaag agcagaggta tctgcaaata gaataaaaga acttttaaat gaagaaccat
     7021 tgataaaaaa tcctgaaaat ggaattaagg atactgaaaa taagggtatc atagaatttg
     7081 ataatgtaac ttttacctat ccagatggag aagcttctgt acttaaggat atttcattta
     7141 ctgcaaaaac aggtgaaaca gttgctttta taggaagtac tggtagtggg aaaagtactc
     7201 taattaactt gatacctaga ttttatgatg taacagaagg tagtattaag ataaatggag
     7261 tagatataag agaatatgat ttaaaagctt taagaaaaaa gataggattc atacctcaaa
     7321 aatcactgct ttttactggt agcatagcaa ataatattag atttgggaaa cataaggcag
     7381 gagagtcaga attagaatat tccgcaaaag ttgcacaagc atatgagttt atatcaaaaa
     7441 aacctagaaa gtttgatgaa ttgattagtg aaggtggagc aaatgtatca ggaggtcaaa
     7501 aacaaagact ttctattgca agggctatta taagaagacc tgaaatatac atttttgatg
     7561 atagtttttc tgcacttgac tttaaaactg atgcaatact tcgtgctaaa ttgaagaagg
     7621 aaacaaaaga tgcaattgta ttgattgttg cccagagaat tagttctatt attgatgctg
     7681 ataagattat agtactcaat gaaggacaag ttgtaggaat gggaactcat aaagaattat
     7741 taaaaaactg tgaaatatac tatgaaattg caacatctca gttaaaaaag gaggaattag
     7801 aataatgaaa aagtctaaag gttttaagaa aacaattggt agacttcttc catttgtaaa
     7861 gaaatataaa ttttccttta ttatagctat tatatgtata atttcagcag ctactatgaa
     7921 tgcacttgca cctaaaactg aaggattgat aataacacaa ttgactaaag atgtaattag
     7981 tatcgcaaaa ggagtaccag gggcttccgt aaattttgat tatgtaacaa aaatattggt
     8041 gattttagca tgtatatatt ttgccaatgc aatttttacg tatacttcaa gttttctact
     8101 taccaatgct attcaaaaca caatgagaga tttaagaaat gaagtcgaaa ataaaataag
     8161 aagattgcct attagttatt ttgatagtaa tagttttgga gatgtattaa gtcgtatatc
     8221 aaatgatgta gatacaatat caaatgcatt gcagcagagt tttatgcaaa ttgtaaactc
     8281 aatcttagtc ataattttgg cactttcaat gatgtttaca attaatatat atatggctct
     8341 tatagctctt tttataattc caattagtta ttttgtatct aaatttgtag taaaaaaatc
     8401 tcaaagtaga ttttcattgc aacaaaatgc tcttggaaaa ttaaatggga aagtacaaga
     8461 aatgtatact ggatttaatg aaataaaact atatggtaaa gaagaagatt ctataaaaga
     8521 atttaaaaaa gtaaatcaag aactttgtga aaatggattt aaggcacaat ttatatcaag
     8581 tatgatgaat cctatggttt cattagtaac ttactttgga attgcagctg tggcagttgt
     8641 cggttctata tatgcagtaa gtggaggaat aacagtagga aatttacaag catttgtgcg
     8701 ttatatatgg cagataaatc agccactttc acaaatgact cagttatcta cagtaataca
     8761 atcttctttt gcagctatag aacgtgtatt tgaaatttta gacgaagaag aggaaatacc
     8821 tgatgttgaa aatccagtta agatagaaaa tgtaaaagga aatgttacat ttgagcatgt
     8881 taattttgga tatggagaaa attcaacttt aatagaagat ttaaatgcag aagtaaaaag
     8941 tggtcagatg gtagctattg ttggacctac tggagcaggt aaaacaacat taataaactt
     9001 attgatgagg ttttatgatg taaagaaagg tgctattaaa attgatggag tagatataag
     9061 agatatgaaa cgtaaagatt tgcgttcaat gtttggtatg gtgcttcaag atacatggtt
     9121 atttaatgga accatttttg aaaatataga atatggtaga tttggagcta ctaaagaaga
     9181 aattatacaa gcagcaaaag tagcaaatgt tcatcacttt ataaccactc ttccagatgg
     9241 atacaatatg tttttaaatg aagaagcatc taatatatct ctaggggaaa aacagctttt
     9301 aaccatagct agagctttta tatcagaccc ttcaatattg atacttgatg aagctacgag
     9361 ttctgttgat actaggctgg aacttatgct tcaaaaagca atgagaaatc ttatgaatgg
     9421 tagaactagt tttgttatag cacatagact ttctacaatt cgcaatgctg acttgatttt
     9481 agttatgaat aatggaagta ttatagagca ggggaatcat gatgaattaa tggaaaaggg
     9541 tggattttat gagaagcttt ataatagcca atttgcagac aaagaatcag aatagataac
     9601 ataaactttt gtataaaatt ttgtatcaaa ttcaaatatt aatcatataa ttaaaaagag
     9661 tatcagtaag ttgttttttt atagatttac tgatactctt tgattttaag accaaaggta
     9721 cttatgattg gtacttataa ttcacaacta gtcttgttac atctgataac cagaattaca
     9781 gtacttttct tttttacctt tttgttctct aacatataac aattcgctta acttatcaat
     9841 gtaataacca tcatcactta tgcgaattaa actagtcaga taatatccat ctttgcttct
     9901 aggaatcttt ttatttattg ctaaatcaat agctgtcttt gctgcttcaa aatgctttaa
     9961 atgtgtatga ccttcttcaa aactctttct agtattataa actacataac cttttttaac
    10021 ttgaaaaact ataaattctt tcttttcata tattttatta aacattcaat cagcccttct
    10081 caagatagtt ttatagcata acattagaag cttctaaaaa attttacaaa gaacataaat
    10141 attttataaa ttataaatct aaaactataa tttataaaga taactttctg attatttata
    10201 tgataattca tatagattaa aagtaaatta tctttaggtt aaacaaaagt aaaataaaag
    10261 actagttata atatttatat gaaaaagtac aaaatatagt atatgaatat agaaaattta
    10321 aattatttat ttacagtttt tatttccata agtatattta ccttcagata ccttgtcatc
    10381 atggaaatat attctttcac aacttttatc tccccataga atgttttttg ttattttatg
    10441 ggacgtatct cctgtaaata cttcagctac agtagtatct gtaacatcac agtaaagaga
    10501 agcgaatttt ttaaaccaag gattttcctc aaatcttaaa agccaggaag ctccataggg
    10561 gcaatgtaac tcttcaaatt caggaaccca tcctagaaat ggaacatcag ttgtgctaat
    10621 aatattgtct gcaattaatt catctccatt ttcatgagct ctctctctca tagcaatagc
    10681 tctttcttca gacctttcaa ataaaacttt tcttaatatt tcgctagttt tatcttcacc
    10741 aaactcttca aataacactt tggaaaaata aaaatataaa tcaccaaaat gtttgcatgc
    10801 catacgaact tcttcaatag cataatcttt gtcaatagtt tcattagtta caacatcttc
    10861 gttcaaaaaa atcactcctt agaataagta ttagttacaa tataattata gaatattttt
    10921 tataaattag gaaattaatt tgagatagat atatttttaa taaatgaaag tataatgcta
    10981 aaataactta ttgttacaaa aatcttctaa attaatgtat ttaggagttt ccatatgaat
    11041 gagagtagtt ctgtaaatat cttcaataca tccatttgca atattattaa aaatatctgt
    11101 gtgctctttt aatgttgttt caagccttcc ttcatgtgac aatgaatcga gagctaattt
    11161 ttttattctg tacatataat tgttaaacat tttgtcaaat tcagtatttt gagcatatga
    11221 aactatagtt gtgtgaaata atgtatcgta ttctgcaaat gagtttaaat ttttatcacc
    11281 taataaaata tctttttgtt tatctaaaag atgttttaat ttacttatag tttctactgc
    11341 tctagcttct ttgtattgac ttgtaattaa aaaagtacaa aatccttcta ttgcagagcg
    11401 tatttggaat atctctacaa tttcatttgc tgtaatttga tggattctaa atcctttgct
    11461 tgggattata tctataaatc catcttgact aagtctttga agagcatctc ttataggtgt
    11521 tctagagata cctatttcac tagcaagttt tgtttcagaa tatatttcat tttctatcat
    11581 ttctccagat agtatcaatt cttttaagta gtcatatgct tgttcttgta atcttttaaa
    11641 tgtagccata cttaataata cccccctaaa caatataaaa atacaaaaac ttatataatt
    11701 ttaagcatac aacatagtga aaataaggtc aatcttttgc aatttaaatt gcagataaga
    11761 ttaaaaaata taaaaaatta ttgaagtgtg aagcatgatt aacattaaag gcagattaat
    11821 taaaactaat tgaaattagt taaaatacaa aactgtaaaa gtagtatatt atatttgaaa
    11881 acaatagtat gtattaaaac ttatatagga tgactaaata taataaatga ctttaaaaat
    11941 tactatttat tgttaaaata aatactttta tattttatat tgaccggtat actggtatgt
    12001 agtatactct aaaacataaa aaacgttttc aaaaatgtga agatatggag ggttataaaa
    12061 tgaaattggg atttattgga ttaggaataa tgggaaagcc aatggcaaaa aatttattaa
    12121 aagatggatg taatcttttg gtgtatgaca ttaataaatc tgctgtggat gaacttatat
    12181 catgtggagc aaaatacgca agtgttttag aaatgggtca agaatgtgat attgtattta
    12241 caatccttcc aaatggaact attgtgcagg atatattgtt tgggatggat ggattggcaa
    12301 aaactttaaa agaaggaagt atagtagtcg atatgagttc agttactcct acagaatcca
    12361 tattgtgtgc aaataaatta aaagatatgg gcttagaatt tatagactca ccagtaagtg
    12421 gaggggagcc aaaagcgatt gatggtacac ttgcttttat ggctggtggt aaggaagaaa
    12481 tttataagaa agtagaacca ttttttaata taatgggaag ctcatctatt ttaattggag
    12541 ataatggtag tggaagtgtt acaaaactta caaaccaagt aattgttaat ttgacgatag
    12601 cagcagtttc tgaggcattt gtacttgcag caaaagcagg tgcagaccca gaaaaagtat
    12661 ataaggcaat aagaggaggc ctagctggta gtacaatatt ggatgcgaag ataccaatga
    12721 ttatgaatcg tgattttaaa ccaggtggga aaatatctat taatttgaaa gatattaaga
    12781 atgtaatgca aacagcacac aatttagatg taccactacc tatgacaagt caattattag
    12841 aaattatgca gacattaaaa gtacatggac atttggaaga tgaccatagt ggaatagcac
    12901 agtattttga aaagttggca ggtgttgaaa taaaaaaaca caataattaa tatttaaata
    12961 tatcaatata attaaataca tattattagg attagattat ataaatacag cgaggttaag
    13021 ttatcatagt aaaagaaaaa taaaattgat aaataataaa gtagaatgat aaaggggtaa
    13081 agattatgat tacaggatat ggacttttga tagcatttgt tatatctata ggaattttac
    13141 ttgtatcgat tattaaattt aaagtaaatc cgtttttggc attactgata acatctataa
    13201 ttacaggctt tatggttaga atgcctataa atgaaatatc aaccacaata tcgactggat
    13261 ttggaaatac attaggtagt ataggtattg taattggact tggaataata tttggaaata
    13321 ttctttcaga atcaagagca acagaatcta ttgcaaaggg gttgttagct aagactggag
    13381 aaaaaaacag tgctttagca gtaactacag caggtttttt aatttcaatt ccagttttta
    13441 tggatgcagc ttttgttata atgatgccta taattaagta tgtttcgaga gtaactaaga
    13501 aatctctaat ggtttttgta tgtgccttag gagttgggac tatagtaggt catgcacttg
    13561 taatacctac accaggacct ttagcagttg cagcaaatgt aaatgcaaat gtaggtagtt
    13621 ttatacttta ttctataatt gttgcttttc cagcagctct tataggtgga tggatttatg
    13681 gaaaacgttt tgaaaaatat ccagcttatg ctattgatga gaatgataga gaaaaaaatt
    13741 tagagcaggg aaaagatagc ataaaaattg aagatgatga ttcttctaaa gtaccaggtt
    13801 ttggaatttc tatgttttct ttattgttcc caatacttct tatacttata tcaaatgtat
    13861 ttagtatgtt cttagaaaaa ggttctacaa tgagtggagt acttgcattt ataggagata
    13921 aaaacatagc aatacttctt ggaatattgg tggcaatagg atttttaaag aaatacataa
    13981 ataaacctat gggagatgta gttattgaag ctgctgattc agcagggcta atattattga
    14041 taacagggtc aggtggtgcg tttggcagtg ttataaatgc aagtggtata ggtaacttct
    14101 tagttgatac tatgtctggt cttagtatat cagttgtagt gcttggattc ttattaagtg
    14161 ccttgcttag aatttctcaa ggttctgcta ctgtagcctt agtaacaaca tcatcaattt
    14221 taggacctac aattttggca actggaatgt caccagtttt agtagggctt gctatatgtg
    14281 ctggaggagt tggtttctcc cttccaaatg attctggatt ctgggtactt tcaagattta
    14341 gtggattatc agttaaagat actttaaact cttggacaat tggaggaacc attgctggag
    14401 ttgtagcatt tataatggtt ttattgctta gtgttataaa tggaattata ccacttccag
    14461 gtctataaat aacaaactat tgtttatatc atataaatta catttagact atacaaatta
    14521 cataaagggg cgttttaaat gagagaagta aaaccaatta gcatagatat acttaatact
    14581 tttaaacaag tagatgagga cagattaaat aaacttcttg cagatgagct taagcattta
    14641 gatagaaaaa ttgttgtatt agatgatgac ccaacaggtg tacaaacagt tcatgatatt
    14701 tctgtttata ctgattggga taaggatagt atggaacaag gttttaatga gaaaaatagt
    14761 atgtttttta tacttaccaa ttcaagggga tttactgttg ctcaaactac aaaagcacat
    14821 aaagaaattt caaagaatat tgtggatgta tcaaagaaag ttaataagga ttttataatc
    14881 ataagtcgta gtgattctac tatgagagga cattatcctc ttgaaactaa tttattaaaa
    14941 agcgaagtag agagattatc ggagaagtta tttcagggtg aaataataat gccatttttt
    15001 aaagaaggtg gtaggtttac tatagataat gtacattatg ttaaagaagg agaaatactt
    15061 gtaccagcag gaatgactga atttgcaaaa gataaatctt ttggatataa atcatctgat
    15121 ataggtgagt ggtgtgaaga aaagacaaat ggaaagtaca aatctagtga tatgatatat
    15181 atttcgtcaa aagaacttag agagctaaat attgaagcta taacagaaaa acttaaaaaa
    15241 gctgaaggat tcaataaaat tattgtaaat gcaaccgatt atgtagatgt aaaggtattt
    15301 acaattgcgt ttatacgagc agtaaatagt ggtaaggaat ttatgtttag aagcgctgct
    15361 gccataacaa aaatacttgg cggagtgagt gacaaggaac tacttactaa ggatgaatta
    15421 gtatcaaaag gtaataccaa tggaggaatt atacttgttg gttcacatgt gaataagact
    15481 acccaacagt tagaagaact taaaaattgt aaatatccga tagaatttat agaatttaat
    15541 caacatttag ttcttcaaga aaatggtcta aaaaatgagg taaaaagagt tataaaagtt
    15601 gtagaggaaa aaataagcag gggtaaaact gttgctgttt atactagacg agagcgtttt
    15661 gacttggata cgaatgataa agataaacag cttatgattt ctgtagaaat atcagatgca
    15721 gttaccagta taattggtat gcttaatgta agaccaaatt ttattatagc taagggtgga
    15781 attacttcta gtgatgttgg tactaaagca ctaagagtaa aaaaagctac agtaatggga
    15841 cagataaaac ctggtatacc agtatggatg acaggcaaag aaagcaaatt tcctaatatg
    15901 ccatatataa ttttccctgg aaatgtggga gagattagca cacttagaga atcagttgag
    15961 atattgatgg ggtagtatat tagtgtattg aagtttaaac gcttaaattt actgagttaa
    16021 aattaaaata aactaaaatt ataaattaag attaaaaata atagtatatt aattagggtt
    16081 tagagtattt tacaaaaaat attctaaacc ttaatcttat gtattagtat ttttattatt
    16141 taacttatgt gtttatttta ttatttatct tttttaattg atttaatatt ttctttgatt
    16201 ttatttaaaa tatctgtatg cttattagag cttatgttgg ggaatgcttt gattatacgt
    16261 ctatgaagta atttgtggtt agacagcaaa gtattaagtt tattttttag ctcatcaata
    16321 cgatttttag catagcgata attttctttt tctttaagta tttcaaacct atcttccaaa
    16381 gtatccagtc tattttcaac taaagattca agtttgtttt ctatagtttc ttgtgtaata
    16441 agtttgtagt attctttctt atcttttaat tccttaatta taagattgat ttgttttaac
    16501 ttaccattta attgtaaaat tgtatggact gtgataacta aatctaaaac aaaataaatt
    16561 tctatagata tagcaaatat aaatagcaca tttttagaga tagagttaat aaagtcaacc
    16621 acaataggat gtactataag cattacaatc aatgataata caccaaacat aagtgagttt
    16681 tccagacaaa ctcttccgtg aatattaaat ttatgtttag aataatccca ccaagtcata
    16741 ttaaatattt tttctaaaat aaagctcgta caatactcca ttatgctagt tataattata
    16801 ctaaacaaaa acaacaaagg aatagtgtgt acaaaaggag tcaaggtagc aactattgct
    16861 aatgctccaa atccatatat agggcaaaat ggaccattta aaaaacctct atttacaaat
    16921 tgtttttgta aaactgaaca atatgtagtt tcacaacacc atcctataaa gctataaatt
    16981 acaaaataaa gaaattgaat ataaaatgta ctcataagca cctcatttct agttagtata
    17041 acactatata ctcgatattt tatttttatg attaatttac taaattcaat ttacatattg
    17101 attataccaa tttatagaaa tttatcaaat ttaattacag ttagaacatt ataaacatgg
    17161 ttataataat gaaattttat ttaaaataaa taagggtgtc aagtataatc aaactaatag
    17221 ttgtagatac tctacactat gttagtttaa ttacatttga cacccttata tttatactat
    17281 gactatttac attcgaaact agagcaacat gtttcaatat aaaggtttac tctatcagtt
    17341 tcaggagcaa ccaatatatt atcaagtgta cataagctac catctttgtt gtatttacaa
    17401 ttttttacag tacaaactaa ttcctcacaa tttctattat taacgttgct agataaatta
    17461 ctataaatag attcatctaa aaatgaacta caacaagtat gtctgtcatt gtgagctttt
    17521 tcaccattta taaaaatttt atctgcataa catatatggc tgttgttatg tgaacagtta
    17581 attacattac agtttattct ttccaaagtt attacctcct aaacgattat ttattataaa
    17641 ttatgtccat ataattacaa gaatataacc attaagagaa aactaccttt tagaaaatat
    17701 ctattcttag agtaatttga aatataaata tattttcttc taaaatttac ctaaattgta
    17761 taaaaactta cagcttggag aaaataatag aaaaaggagg cgtaatcaat gtttgaaaag
    17821 aagaaaaaag aattatttcc ttggaaaata tctgccttaa tactggttgg agtatttgta
    17881 ttaagtagcg gagtttatat agggacaaga ttaaaacaca atggagttca aactggtgaa
    17941 gttgtaagta ctagtgcgaa tatgaatgaa aagtcaaaag aagcttttgg attaaataaa
    18001 aattgtgaag tttggcttca aagtgaaggc gaaaatgaaa ctaactataa gagaacactt
    18061 acaatggcag gaatagttcc agagtcatta ttagataaga cgaagtcaga aatagtatca
    18121 tattttaaaa aagaatatcc aactaaaaaa ataaagagta tggataaaaa tgaaatcata
    18181 ttggtagaat ctaaatatag tgataataaa gatgtacaag caatggatgt tactataaaa
    18241 ggtaaatata ctattgaaaa tgataatgga aatatagcac tatataaata tgatgatagt
    18301 ggaagcaaaa cattagtaga aaaaacaaaa ataagagtag attcacttcc taaaacagtt
    18361 caagatgaaa tcaaaaaagg tgtagttatg gatactgaag aagaggccta ttcaagatta
    18421 gaagactttg caagttaata gtaaattaat attattttaa gttaaatact aagtaagtta
    18481 aatactaagt aaattaataa taaataattt aatattaaat agattagtat taaatagatt
    18541 agtattaaaa ttgtagtcac atttaattcc tataaaaata caataattta tttgtaataa
    18601 atataaaagt tatattgagg taaaatcttg ctttgatata acttttttat ttgattttat
    18661 attttacata gagcatttta tgatttgtta atattattta tgatataatg atttagaaca
    18721 taagttttta tgagaaatac attttggtat ttcttttatt tttaggggga attttaaaaa
    18781 atgataatat tggggattga cccaggtata gccatagttg gatatggtat aattgaatac
    18841 aaaaatagca agtttaaagt aatcgattat ggagcagtta caacacctgc ccatatgaat
    18901 atatcgagaa gattggaact tgtgtataaa ggaattgata caatagtaaa gaattacaat
    18961 atagatgaag ttggaatgga agaattattc tttaacaaga atgtaaaaac agctataaca
    19021 gttgcacaag ctagaggtgt tactatgctt gcatgtgctc ataatgggaa gcctgtatat
    19081 gaatacactc cacttcaagt aaaacaaggt gtagttggat atgggagagc agataaggca
    19141 caagttcaac agatggtaac ttcattttta agtctaaaaa aagttccaaa accagatgat
    19201 gttgcagatg ctttagctgt ggctatttgc catgctcatt caaacaaact tgaaaaaact
    19261 ctaaagaata taggtggtaa gtatgtatag ttatataaaa ggtacagtag aagaaattta
    19321 tatagacagt atagttgttg aaaataatgg gataggatat aaaataaatg tatcatccaa
    19381 tacaataatg aatttacaag tgggagaagc cacaaaaata tatactaaat taattgtaag
    19441 agaagatgat atgagtcttt gtggttttgt aagtagggaa gaacttaaga tgtttgaatt
    19501 actaacatct gtatctaaaa taggacctaa agtggcgctt tcaatactat catttgcatc
    19561 accagcacag ttaggagcat atattttaag tgaagacatt ggaaaattat ccaaagctcc
    19621 tggtgttggt aaaaagacag cagaaagaat cgtcttagag cttaaggata aggtagacaa
    19681 aaataacata gagtttgaac caacattatt atcacaaaaa cctaccttga tatcacaaga
    19741 tgagtctgta gatgcacttg tagctttagg ctatactttg tcggaatcaa aagaggcagt
    19801 tcaaaaatgt aaaaaagatg gtatgaatac agaagctatt ataaagaaag cacttacata
    19861 tataatgagt aaatccttga aatagtattt ggattttata atatttggat tttaaaacaa
    19921 tatttaagga gtttatgtaa atttgaaaat atataaagta gattttcaaa agtatgagag
    19981 gaggggtata aatgcaaggt tttgaagatg aaaataggat aataacttct acaatgaaga
    20041 tggaagatat agatatagaa aatagtttaa gaccaaaaac tttagaagat tatttgggtc
    20101 aagaaaaatc caaagaacaa cttagtatat ttatagaagc agctaaatct agaaatgagc
    20161 agttagacca tgtgctttta tatggaccac caggtcttgg aaaaactaca ctagcaagta
    20221 taatagcaaa tgagatgggg gtaaatttaa gaattacgtc aggacctgca atagaaagag
    20281 ctggagattt ggcagccata cttacgaatt taaatgaaaa tgatgtttta tttatagatg
    20341 agattcatag aatcaataga agtgttgaag aagttttata tccagcaatg gaggattttt
    20401 gcctagatat aataataggg aaagggccat ctgctagaag cataagactt gaccttccta
    20461 aatttacact tattggagct actacaagag ctggtatgct tacaaatcca cttagagata
    20521 gatttggtgt tatttgtaaa ttggactatt atacagttga tgaactttca aaaatagtac
    20581 ttaggtcttc aagcatctta gatgcagaga tacagtctaa tggagcatta gaacttgcaa
    20641 aacgttcaag agggactcca aggatagcaa atagattact aaaaagagtt agagattttg
    20701 cacaagtcag agctgatgga aaaataactg ataaggtggc aaaagatgct ctggaacttt
    20761 taggagtcga tagtttgggt ctcgattttg tagatgagaa gcttcttatg actataatag
    20821 aaaaatttag aggaggacca gtgggtttag atacattggc agcttctatc ggagaagata
    20881 gaaatacaat agaagatgta tatgagccat atttgcttca attaggattt ataaatagag
    20941 gtcctagagg aagagtagct atgccattag catatgaaca cttaaaaatt ccatatccta
    21001 atgaaaaata aaaatagatt atacgagtat ttataaaata aaagagtcat taaattaaaa
    21061 tatttctaaa atggctaaaa attcaaacat atccacaaaa tggtaattat atctgtagat
    21121 actgtggata aacacgataa aaattgtgtg tattgtatct atatattgtg gataagacat
    21181 gtatatcgtg gaaaaatatc tatatattgt ggatatggtg tctgttaaaa atagcaattt
    21241 cttgatagat tatggtaata ttgtggaaaa taagttgttt ttttgatatt attgtagctg
    21301 gagaaatttt aagagaggaa gtttaacatt gaaaacaagt gattttaaat ttgacttacc
    21361 acaggaactt atagctcaag ttcctataga agatagagct agctcaagat tgatggtatt
    21421 agataaagag acaggtaata ttgaacataa agtatttaga gatataatag aatatttaaa
    21481 tccaggagat tgcttagtat taaataatac tagagttata ccagctagat taataggaga
    21541 gaagcttgaa actggaggta aaatagagtt tttattatta aaaagaacag aggaagatac
    21601 ttggcaggct ttagtaaaac caggaaaaag agcaaaagta gggactaaat tttcttttgg
    21661 aaatggaaag ctaattggag aagttgtaga tttatcagat gaaggttcaa gaattataaa
    21721 attccattat gatggaattt ttgaagaaat attggatgaa cttggaaata tgccactacc
    21781 tccatatatc actgctagac tagatgaaaa agaaagatac cagactgttt attcaaagca
    21841 taatggttca gcagcagcac ctacagcagg attacatttt acagaagaat tgttaaataa
    21901 aataaaagaa aaaggtgtag atatagcctt tgtaactcta catgttggct taggaacttt
    21961 tagaccagta aaagtagagg atgttttaaa tcacaagatg cattcagaat attatatggt
    22021 tagccaagaa gcagctgaca aaataaatag agcaaaggaa aatggaaaaa atgtaatatg
    22081 tgttggtact accagttgta gaactataga atcagcttgt aatgagtatg gaaagatgaa
    22141 agaaactagt ggatggacag aaatatttat atatccaggt tataaattta aagttttaga
    22201 taaacttatt actaattttc acttaccaga gtctacacta ataatgttag taagtgctat
    22261 ttgtggaaag gataatgttt taaatgcata taatgaggca gtaaaagaaa gatatagatt
    22321 cttcagtttt ggagatgcta tgattataaa gtagggaggt aatggatgca tagtttacaa
    22381 aaacaaaaaa agaataaccc tatattaaag attgctttta tatttgaacg tgttttagca
    22441 gtagtagttc taatagcagt atttttagga actatagatg ttctaagact tatgtgggga
    22501 gcatatatag ttgattttca aaatccagta caatacagtc agcttaatga ttttttagca
    22561 caaatattac ttttagtaat aggtgttgaa cttgtagtaa tgctatctct tcatatacca
    22621 ggagcattta ttgaggcatt actttacgca attgctagaa aaatgttgct actgcctaaa
    22681 aatgaaggta tgatagatgt tttaatagga gttatagcta tagcaggact atttgctata
    22741 aaaaagtttt tagtaacaaa agacaagagt gctcttaata taatgagtat tcatgatgaa
    22801 gaagcaatta aggaacaaca gaacaaagaa gacgaagata aatctattta gacaataaat
    22861 tatatgaata gcttattaca aatatatctt aagaaataat gagaaataaa tatgattaag
    22921 gagaacttta tgtacgcagt aagatacgaa ttgataaaga cttgtaagca gagtggtgca
    22981 aggttaggaa gattacacac tccacatggg attattgaaa caccaatatt catgccagtt
    23041 ggaactcaag caactgtaaa atctatgaca ccagaagaat taaaagagat tggttcacaa
    23101 ataatactta gtaacacata ccatttatat atgagaccag gtcatgaact tataaaaaga
    23161 gctggtggtc ttcataagtt tatgaattgg gataaaccaa tacttacaga tagtggaggg
    23221 ttccaggtat ttagtctagg gcctttaaga aagataaaag aagaaggtgt agaatttagg
    23281 tcacacttag atggttcaaa acattttcta actccagaaa aagctatgga aatacaaaat
    23341 gcattgggtt cagacataat gatggcattt gatgagtgtg caccataccc atcagataga
    23401 gaatatgtaa aaaattcttt agaaagaaca acaagatggt taaaaagatg taaagatgcc
    23461 cataataata ccgacaagca agctttattt ggtataatac aaggtgggat gtataaagac
    23521 ttaagggaac aatcagcgaa agaaataact aatattgatt taccaggata tgctattggt
    23581 ggacttagtg ttggagaacc aaaaccactt atgtatgacg tgttagagca tacaactcca
    23641 cttatgccaa aggataaacc aagataccta atgggagttg gtagtccaga tgacttagta
    23701 gaaggtgtta taagaggagt agacatgttt gattgtgttc tacctactcg tatcgctaga
    23761 aatggaactg caatgactag ccaaggtaaa gtagtagtta gaaatgcaac ttatgctgaa
    23821 gactttactc cacttgaccc tgaatgtgat tgttatgctt gtaaaaacta ttcaagagct
    23881 tatataagac accttataaa agcaaatgaa atactgggtg caagattaat aactactcat
    23941 aatcttcact ttttattaaa tttaatgaaa caaataagac aagcgataat ggaagataga
    24001 ttacttgatt ttagaaatga attttttgca aaatatggat atgaaatata ggttttgcac
    24061 tattaattat aatcatttta atatataata ttcaatagta ctcaaagata gagaaagtaa
    24121 attgttatat aaacatttat tatgatttac aaaagagtat aatttacaaa ggagtgaaaa
    24181 attaatgcca cagcaacaaa taattatgag tataggtcta tgggtagtag taatcgcaat
    24241 cttttatttc ctaatgatta gacctcaaaa gaaaaaagac aaacaattaa aagaaatgag
    24301 aagcagccta agtgtaggag acaaggtcat tacaataggt ggaatagtag ctaatgtagc
    24361 taaagttgaa gatgatgttg taatattaga attaggacca aatagaacta aagttccatt
    24421 tgaaaaatgg gctataggaa ctgttaaatc taaaaaagaa gaaatagaag aagactaaat
    24481 tagtaattta aaagcctttt ctcgaatata tattaatttg agaggaggtt tttttatggg
    24541 aaaatctaat tatattttta aaggtctagg atatgcatat ataataactt tggctgtttt
    24601 attagtgtat aacttatttt tgacatttac agatattggt ggagataata taactatggt
    24661 ctcatcattt ataactacta tttctgcagc tataggaggg ttttacactt caaaacacat
    24721 gaaagaaaaa ggacttatgt atgggctttt agtaggactt ttgtacatag tatgtatttt
    24781 tttgactgtg tttttagcac aagaaaagtt tgtatttgaa atagggatga tttataagtt
    24841 attgctaata tcagcagcag gaggcattgg aggcgtattg ggtgtcaact ttaaatagag
    24901 tttatattaa agtagtttta tgatataatc aataggaaac aattagaaat ggaggcgttg
    24961 aaaatggaaa ataaacatgt aaaaacttta tctcaagcta ctttaaagca aagtgctgca
    25021 aaaggtggat gtggagagtg tcaaacttct tgtcaatcag cttgtaaaac ttcttgtaca
    25081 gttgcaaatc aagagtgtga aagatagaaa attattgatt tagttgtttg taagagggta
    25141 tctcaaagtg attcttttat cattatgagg tatcctcttt tattttcaat tattatataa
    25201 aaatatatat tctatttatg tagaagtaaa tttgatttct tatcgtttaa ttatgcaaaa
    25261 atatctaatc atgtaagagc atttatttta aagtagctac tcttatattt tattttttat
    25321 caattacatg tattagaata atttttaatt ttaaactcta atttatgatg atttttataa
    25381 tatgttatat attttaatat atggagatag atattaattt agtgatatat atttaagtaa
    25441 taagagtaaa gcaaaaatta gtgcatagta gtatataaaa atggagtttg aatacttttg
    25501 ttagtatatg attgtttagt agaaaatcac tataaaatag gatttctatc tttgagcata
    25561 attatagaga gcctaagtag ggggaagggt ttcaagaaga tattattagt gtttgtgtta
    25621 gtaaattatg taaacacata aaatatattg aaaaataata ttatatataa tattatttat
    25681 gtgtgggaaa tacgaatata aaaaataaaa aataatattc ggaggacata aatatgttaa
    25741 gcaacaaaaa gagaagtatg gcaatagtaa tggcaggtgc tacagttatg agtgcagcag
    25801 caccaatatt cgcagacaat accgtgacag aaaatgttga taaaaattac acagtaagtg
    25861 cgaaagattc tgctaaacta atcgaagagg ttagaaaagc attagaagtt aagtttgaag
    25921 atacaaaagc aggagcaaat gtaaatgata gagtttacga tataaaagta gacaatgtaa
    25981 acttaactaa tgcaactcaa ttacaaaata aaataaactc tttaacagaa gggcaaagtt
    26041 taaaagttac tattcaagat aaagggcatc aagtgttagg tggaaaagta gttgactata
    26101 aaattgaaaa ctacaaaact gctcaagaaa tagtagatgc agttaatgct tacaatgcaa
    26161 ctttagcaga agattctgat aacaagttaa cagcaactat aaaatctaca aatacagttg
    26221 aagtaaaaag agcaaaagat tcagcaaatg ttattacatt aaatgtagga gaccaacatt
    26281 tagatttctc aaaagtgata acatcagaag aaggtacttt tgaaggttat gaaaagagtt
    26341 atagtgacat agattcaaaa gaattacata cgataacagt taaaaacgca gatttacaag
    26401 acatatcagc agaagaatta tttgatggaa ttagattaac tacattagga agagaaatag
    26461 ttaataaagt taaaaatgga tatgctttaa catttgaaaa tgaagcaatc cttactcaag
    26521 aacaagaaga tagtgatgat aaagataaac cagaaaaatc aagctttgat atagttttaa
    26581 gcaaggcaaa tgaaaagcca gaaacaataa gtgtttcaag taaaaaccat aagttagtta
    26641 gagacttaca caaagtatta actgatgtaa aagatggaaa agaattaaaa gtagaagttt
    26701 tatctggtga ttcaagattt acaacagcag tagaagttag taaagagaga tttaaagatg
    26761 gagaagcaga agctataatt ttagttggag aagatgctat agttgatgga ttagcatcag
    26821 caccacttgc ctctcaaaaa aatgcaccaa tattattatc taaaaaagat tcactaccat
    26881 cagaaataga agctgaaata ttaagagtac ttggaagtaa tctatcttct aagaaaatat
    26941 atatagtagg tggagaatct aaagtatcaa aagaaactga agaaaaactt tctaaattag
    27001 gtgtaagtaa agttgagaga gtttctggag aagatagatt tgaaacttct ttagaaatag
    27061 caaaacaatt aaaagataca tttaagactg cttttgtagt aggtggaaat ggagaagctg
    27121 atgctatgag tatatcagct agagctgctc aatttggtgc tccaataata gttacaggta
    27181 acgaattaga tgcaaatgct gaaaaattat taaaaggaaa agaattagaa atagtgggtg
    27241 gagaaaattc tgtatcaaaa gaagttgaag acaaattagt tgatatagat ttaaataata
    27301 aagttgaaag attagctgga gaaaatagaa aagatactaa tgctaaagta atcaataaat
    27361 actatgcagg tgcaactaaa gcatatgtag caaaagatgg ttatgtaggt ggaaatggac
    27421 aattagttga tgcacttaca gcagcaccac ttgcagctag ttcaaaggct ccaatagtat
    27481 taactacaga agaactttct aaatcacaag aagaagtagt tgagttaaga cttaagaatg
    27541 ctactaagtt agtacaaata ggtgaaggaa tagctaaaaa cgctattgaa aaaatagcag
    27601 aaaaaataaa cttatttact aagaactaat aagatatagg aattaatatt aaaagtttat
    27661 ttagataata tatgattata caatgcgtta atatgtaaga catagtgtag atattataaa
    27721 atatacaaat aacaagataa aataataaaa ataaggtgtc tcatttagaa ttagattgag
    27781 ataccttatt ttattttttg attataatgt tgagaaattc atattgcaaa aattattata
    27841 aaaagatact tcttataatg attaaacata ttactaacat aaattatttt gaacttataa
    27901 cgtaattaaa taaaataaat tataatgttt tgaatttata gagaataaaa actttaggta
    27961 tattgtttga gttgtatgga taactatggt atcatatata aaatatggta acaaaattgt
    28021 aacttttgat taaaaataag tatgagtact aataaaaaat atgaaaagta cattgggggt
    28081 agaaaataat atgaagaata gtaaaaagat attagctata ggacttacac tatttttagt
    28141 aatggtaaat actcctatgg taaatgcgtt gacatcagtt gagcaaataa aagggaatga
    28201 cagatatgag acagcagcaa aaatagcaga taagcaaaac tacaatacag caatactaat
    28261 caactcagat aatagcttag cagatggtct aagtgcaagt ggtttagctg gagccttaaa
    28321 tgcgcctatt ttgatgacaa aacaaaacca gattccaaac acaactatgg aaagattaaa
    28381 caaagcaaag actgtgtata taataggttc agaatcaaca ataagtaaaa atgttgagaa
    28441 tcagttacta tccaaaaaga aggttgtaca aagaatattt ggtgaaaata gatttgatac
    28501 aagtataaaa atagctgaaa aaatcaaaga aattaagcca atagataaag taattatagc
    28561 caatggattt acaggagaag cagatgcgat aagtgcatca ccagtagctg ctagagatgg
    28621 agtacctata atacttacag atggaaatag tgtgggattt gacacgacag gcttaaagag
    28681 ttatgcgctt ggttctagtg aaataataag cgacgaatta gttaagagca caaattctat
    28741 tagattgggt ggcacagata gatttgaaac taataagata gttatacaag agttttataa
    28801 aaatagtaaa gaattttatc taagtaaagg cttacaatta acagacgctt tggctgcctc
    28861 tacaatagca aaaaatgctc ctgtagtttt agttgaaaat ggcagtaaca agagtatttt
    28921 aagtggtgca gacaaattga ctgtactagg aggcataaat cagaatgtta tcaagcaatg
    28981 tataaatcaa gcttcaccaa atcaacaagg tttatattac aatccaaatg atagagcatt
    29041 taaagagaga ataaagggta aagtatatgc tcttacaaag cagtatagaa aagagaatgg
    29101 agtaagagcg ttatctgtag caagcagatt agagggtcta gcaaatgatt ggtctaattt
    29161 aatggcaaat aagaagactt tatctcatac gataaatgga aaaaattcat attctacttt
    29221 tttgaaatac ttagattgga gtgagattaa acctggatat atagcagttc aaggtgaaaa
    29281 tataattaaa tataaaattc cagataaacc tgtatataca aatagagatg cagatgatat
    29341 aggaaacttt atttttaatg agtggaagac aaatccagaa gaaggaacta atatgttgca
    29401 taaagggtat gaaataatgg gatttggtat agctataaca ggagataaga atctatatgc
    29461 tacacatgaa ttttatggaa gatataaaga ataagaaaat atacacaaaa ttatgcttgt
    29521 aaggaagcta tataaataat taaattataa tggaaaatag catttatatt aataaattaa
    29581 tataaatgtt atttttttat aatttaatct aaaaaaatta tattaaaaag tttaaatata
    29641 gtataaaatg ggtaagaaag tagtgaaaat taaatggcaa aattagggaa atctaatgac
    29701 gcaaaactat agggattaaa gcttttaaag ccatatcagc cagttgctaa aaagagtaaa
    29761 gtctttttat tttataatta agattctctt tgagtgtctt tttttattgt ctgaaactta
    29821 tagtatttaa attcatgaaa aattagatat ttattaaggg aggattgtta tttcatgaaa
    29881 aaggcaatat cttgtgtact agcagtatct atgtgtagtg caccactaaa cgtttttgca
    29941 gaacctatat tagaggggaa gctaagggca gttgaaaaat cagttacaga aaagttaagg
    30001 gggagtcttg aagtagattt aaatttttca ctgccaataa aaaatagcga gtcagaaagt
    30061 atgactaata tgcagttacg attaaaagat gatagtaaca atagtggaat aataaagctt
    30121 ggagaaaaag attcaggaac agtaaatgtt gggacagata atatagaata tactataaaa
    30181 aaattatctg caagtagaag tgaaataaaa aataaagatg agaatatatc atattataat
    30241 attgtattta ataatttacc tgttggaaaa tacaatgtag aagtctctgg agatggattt
    30301 aagaacaaag aaattaaaga cattgatata tcaagttatt cacaaagagt cttattaagc
    30361 aatagagcaa gtgtagataa taaaaataaa cctttaaatt atgaattgtt cttaatgggt
    30421 gatgtaaatg gagacggtaa agtagataag agtgattata ataaggtttt agagaatata
    30481 gattcaaata aaagagaatt tgatttaaat agagatggaa aggtagatat tgttgactta
    30541 gattatgtac agaaaaattt aggacaaagt gaagatgcaa aaagcctaga aagtatagta
    30601 tctaccaatc ctatagtaga tacaagtaag gttgaattaa aaggaaatag tgatgtagat
    30661 attaatggta atgtagaaaa tttatttagt ggagaaaata atggagttac aatatcttca
    30721 aaaaatgatg aaatatctga ggaaaaacca gcagtaatgg atatagcttt atcagaagca
    30781 cttaatatgg aagtcatcaa tataaaagct ccagaaggta ctgctccagc aaaaggaata
    30841 gttgaggtta ctgacaaaga tggtaatgtt caacagattc cttatgaaaa caaagaatat
    30901 atgaaaaact taaaagatga ggagcaacaa gaggaagtaa ataacgaaca agaagaagta
    30961 aatcaagaag aagtaaataa taaagatgaa aatataaacc agactgataa taactcaaat
    31021 aataaagatg aaaataatga taaacaacag gaaaatagcg ataaacaaga agcaaactct
    31081 aataccaatc aagataaaaa taatgataaa gaaaattcat caatggataa acctaattca
    31141 gatgaagata ctccaaaaga agagggtaat atcagtaaag atgataactc tacaacatct
    31201 acatatagta ggtcagcatt agaaacatat gcagatgttc aaaatagtga tatagtaata
    31261 aatcttggta aacaagtagc agtaaaaaag ataactataa aaataacagc tacaacagca
    31321 aacgatagaa atcttgcaga aatatcaaaa gttgaatttt taaacaatgt atataaagaa
    31381 ataccaaagc cagagatgaa catacctaaa ataaataaaa tatcgactag cactgctgtt
    31441 ggaaatgaaa aagtatcttt ttattggaat aatgaagtaa atgtatcagc atacgaactt
    31501 atactagaca aaattgatag taagggaaaa gtattatcta ctaaaaaatt acaaactagt
    31561 aaaaatacaa tagaagttag agatatagaa gcatatggac tatatagagt tagcatacaa
    31621 tctttaaatg gagattggtc tagtggttat aaagatgcaa caccagaaaa cttagacgga
    31681 gtaccagaaa atgtagattc aaactataaa cctatacaat ttagcccaga tagtatatta
    31741 gaatttcaag ttgttccaga cacaaaacca gaaccaccag aaggtataaa tgttaaaggt
    31801 aaatttaaat ctcttgatgt aagctggaaa aatcataaac aagctaaaga ttttgacttg
    31861 tactataaag aaaaaggctc taatgggtct tggataaaat acaatgataa gcttattcgt
    31921 gggacaagtt caaagataga aggtcttaag gataatacaa catatgaagt cagaatgaca
    31981 gctactaacc acttgggaac tagtggaatg tctaaaacat atataggtac aactgaaagc
    32041 atggatgcac caacaacacc taactataga ttaattaata ctcctaaaga aaatggtgga
    32101 tttggaatac caacaaatca cattgtagat gttgtttatc cagatggata tgacaaaagt
    32161 gaatatagtg ataaaaatcc atttgataaa ttcaatgtag tagatgggga ctttacaaca
    32221 cattggacat tcccaacata taatgcagct gagggaacga atagaggacc aataataact
    32281 tttgataaat cttatactat ggatactttt atggctacac ctcgttttga tgtttctgat
    32341 aagacaataa atgactttga ggtacgatat tgggatgaaa atggtcaatt acacaatctg
    32401 ggggatgttc cttttgaaac aaagacagac cctgtaaata ataataagta tgtaatggtt
    32461 agattaaaag aaccaataac tgctaccaag atacaagtaa gtgtttgttc atcctattca
    32521 tataagtcta atatatcaga acttaagttt tatgagtatg acagtataga agatgatgta
    32581 agaaatctat ttatggatgg cctacaagtt gaattaaaag aagatgtaac tcaagaaaaa
    32641 ataactgaat taaaagatag attaaatact ccagataaag ctagtggaga atatcatcca
    32701 tataagacag ttattgagag agaattaaaa ttagctcaag acttatataa tgatagagcc
    32761 acacttactg atgtaactac tgtaaatcaa gaaataaata aattaggaaa atataaagat
    32821 aaaaacggac agtatactgt aaattccaat aatttaggta tgcaaaatga ctggcaggca
    32881 ttaggagtag ctgctagagc aggagatgaa atcacagtat atgttggaag taaaagtgga
    32941 aaaactccta agttgatata tacacagtac tatggagaat ctggtgcata taagagtgga
    33001 gaaataaatc ttaacactgg aaaaaatgta attcaattaa gcaaacttca tagtttagat
    33061 atagaatgtg gaggaagttt atacataaga tacgctgaag atactccaag tggaggagat
    33121 ataaaagtac gtgtagcagg agctactaag ataccacatc ttaatctaaa tggaacaata
    33181 aatgataaga gtcctgcggg agtttctgaa tctaagaaaa agataaaagc atatatagaa
    33241 gaattagcta aatataaaga tgctgtaaaa ggagaaccat attatccagg aacaaatgtt
    33301 ggtaattcat atggatattc agaaaaaact ggtgtgttaa acactacaga tattgaaagt
    33361 gataaagtaa cattaaatgt tcctgcaaca gcagtatatc aagagatatc aaaaggaaat
    33421 tctgatttaa atacacaagt agatagatta tataatagta tgcttgcatg ggaacaaata
    33481 atggatttag tttactcaga aagaggagta tttaaaacac aagatttaga tggtaatgga
    33541 actgtagatg ataatgaatt taacttaact aaaaatgata tggctccaaa atctcgtatg
    33601 aacataaaat accaaagaat gtttattgga gcatttatgt atgcttctgg attacatgta
    33661 ggagtagaat atggttcagt tccagggctt atgaatggag tacctttcca aatagatgat
    33721 agtggaaaag ctacaggagg aaacttattt ggatggggaa taggtcatga gataggacat
    33781 gttacagata ttggaaagat gacatatagc gaaactagta acaatgtact tgctttactt
    33841 gctcagacct ttgatgataa gacacattca agacttgaag gttcaacaat ggataagata
    33901 tatgagcatg taacttcaaa tagcttaggt ataccaagca atgtatttga aaggcttgga
    33961 atgttatggc aattacatct agcttatgat gatgatttta cgggttcaat gttaaagaat
    34021 aactcagatg ctgatttaag taatgataca ttctatgcaa agatttcaag aaaatacaga
    34081 gcactttcaa gtagtgaccc aattaatagt ttaccaaaag accaaatgct agtagcaatg
    34141 gcatcaagtg ttgttgaaaa agatttaaga gattacttta aggcatgggg agtagaaatt
    34201 acaccagaat taaacagtat aatggatagc aagaactacg aaaaagaatc aagaaagata
    34261 caatatgtaa atgatgaagc tagaagaaag attttaaatg aaaatatagc tagtatggca
    34321 aaagatacta aagtaagtgc tagtttttct gatggaatac aaagtggttc tctagttaaa
    34381 tctaataaaa tatctataga cctagatgta gataaggata aagataagat actaggatac
    34441 gaaataataa gaagtgatgg aaattattta gatggtgaaa atggaacaca ggtaaaatac
    34501 agaacagttg gatttgtgga tgcaaaagga aatagcaaga ctaaatttac tgataatata
    34561 tctcctctta ataacagagc atttacatat aaggtagttg catatgatta tcacctaaat
    34621 ccaacagaag aatttgaagt tggtactgta aaattatcag atgaagggaa aataaataaa
    34681 tcagcatggt cattcataac aaatactgtt tctgacggtg atgttagaac tgaaaatgac
    34741 tcacatggac cattacaaaa tcctgaaatt gataatataa aagataatga tgcaagtact
    34801 acatataaag gaaagataat aagtaaagca gagtggaaca aagaccctca aaaagaccct
    34861 gatataaatg tagaagaaaa tccatatata atagtagaca tgaaagaaac attacctata
    34921 gtaggactaa aatatacaaa accagagaca gaagctagaa agttctcatt aaaaggatta
    34981 tttaacttta gaaagaatac tagcacaaca tacaatcctt taactaatta caagatacaa
    35041 gtaagtaatg ataaaaagaa ctgggaagat gtaagtcaag gaacttttga atatggacaa
    35101 gatttagttg gtggaggaaa agaccaagat aatgaagcta gagttacatt taataaagat
    35161 ggtaaacttt ggacatacca agctagatat gtaaaactaa tctcaaataa taagtctaat
    35221 atagaagttg cagaattaga tataatagga cctccaggtg ataatataga aattggcata
    35281 ataaataatg cagacgaaac tcgtactaat ggaattggaa aattagcaga agattatgta
    35341 tatcaaagtg atgactctag cacaagtgaa gatgaaacag gtaagattcc agcgggttca
    35401 atagttatta caggtgaata cagtggaaat ccagcattta atttaccact acttatagat
    35461 aaaaacaata agactatatc tggagaagca cttttatttg cagaagttcc agaaaaaggt
    35521 gaactaggtg aaatcagtaa aggtacatgg gtttactata tgccaaaaga aaactttgat
    35581 tctttaagtg ataaggttaa ggctgaacta tatagataca atgaccttca aggagatact
    35641 ccagtaggtc aaagatttgt aagtgataca atgtatgtac ctgtaggagc taagaattat
    35701 gatgagttaa agacaataag tcttactgat agtaatagta aagctagaaa agctacaaga
    35761 aattctattg atatcagtaa taagaaggtt ataactgtaa gcaataaagt aaagggtaat
    35821 attgttagag ataaatagta ggtggaactc taaaaacgta aaaatactaa cattgtatat
    35881 ctaataagat taggtactag aatagtttta aaatagtaga tattaaaagt aaagtatata
    35941 aatgtaacca taaatttagg gtaatttata aattaaatat aaaagctagg tggataagta
    36001 aatttatcca cttagttaat gatagataag gacgggtgtt aatgagtaaa agaaaatctt
    36061 ttataagaac tatagcagta tcaactatgg cagtagctgt aactggtagt gctacatgtg
    36121 catatgcagc tccagtttta caaggaacaa aaacgtatga gaaagtaaat actatagata
    36181 tatctgtgga ctcagtagaa aacatagtat attcatttca agccagtata aaagtacaag
    36241 gtgaggttga agtagtagat aatgagcaga aagaaaagat tacttggtct gataatataa
    36301 aatctcagat aaaaagtgga aatgcaaatg ctacttgtag agtagaatac aacaaatcaa
    36361 gtaatacaac tacattagat atatatgtta catcaaatga agatttacta gacggaaata
    36421 gattgaatat aggaagaata tctgtaaaaa aatctggcag taactcaaat gctgactata
    36481 aagttttagg aaaaggaaca aatgataaac cagctttaaa aattgtaaca tataacaaca
    36541 aaacagtgga ttatgaaaat ataagctctg atgaaggatt aatatttacc cttattaatg
    36601 agagtgaagt aaaaccaatt ggtggaactg gttctagtaa aaatgaccct gaaaagtata
    36661 aggtagagaa aagtgaagca cttgaatatt tattaaacaa tattaggata aactattcta
    36721 ttgttagtaa agaaacacaa gaaagtggtt caaatgtaat attaaaactt ggattagcac
    36781 aaaaaacaac taagggaaga aaagctacaa taaacaaata tgttgaagtt acacttccaa
    36841 aaagtttaga atacatagtt gaaaatgaat tatcaaaacc ggatgaatta ccaccagata
    36901 atggcaatgg tggaaataat ggtggaggaa gtaattcagg cggctcatct agtggtggaa
    36961 gctctggagg aggaagttca agtgattcta catctaatgt cacagtcaaa aaattaaaag
    37021 gtgctgatag atttgagaca gctatcaaaa tatctcaaag tggttggaca aaatcagata
    37081 cagttgtaat agtaaatgga gaagataaaa gtatggtgga tgggcttaca gctacaccac
    37141 ttgctagtgt aaaaaactca ccaattcttt tatcatcaaa tgaaaagctt cctcaaaaaa
    37201 ctgtagaaga attgaaaagg ctaaatccat caaaagtaat tgtgataggt ggaaacaact
    37261 caatgccaaa ttcagttgtt gaagctatca aagctgtaaa ttcaaaaata tcagtacaaa
    37321 gaataggtgg agatacaaga tatcaaactt ctattaatat agcaaaagaa atagataaga
    37381 cgaataatgt aagcaagttg tatataggag caggaaatgg agaagctgat tcattatcaa
    37441 tagcatcact ggctggtaaa gaaaaaactc caatagtact tacacaaaaa gatggtgtag
    37501 ataatgaagc tgaacaattt attaaatcaa ataaggtgtc aaatatatat tttattggtg
    37561 gagtagaaaa aatatctaac aaggctattg aacaagttgg aaaaatagca aacaaagata
    37621 tatcaaacaa tagggtggca ggtcaaacta gacaagagac gaatgccaaa gtaatagata
    37681 aattctactc tcaatctaag ctagatggag ttgtagttgc aaatcaagat aaacttatag
    37741 atgctttagc agttggacca ttagcagcta aaaacaattc accagttata cttgctacaa
    37801 atacgttaga taaatcacaa gaatcgtcat taaaaggtaa aaattcatca aagttgtttg
    37861 aagtaggtgg aggaatagcc tcatctgtaa tagacaagat taagagttta attgaaaaat
    37921 aaatattaag taaatagaaa ataagaatgt atttagatat aaagttatat atttaaatgc
    37981 attcttattt tattttcatt atcttaacaa tctaataata ttgaggttaa aatgtataat
    38041 aatttgagct atattaagtt taattttaaa taaagcagat atcttttata tatataaata
    38101 taaattcaat tttgaatatg tttaaaaaga agttaaaaat aattttttaa atttaataaa
    38161 ataaaacact aatatataga cgagatatat tcatatcata tatactctat aatatatgga
    38221 aaataaagag aaaataaatg gaaaataatc acaatataaa tataaaatat aaaaatcatc
    38281 agggggatat gaaaatgaac aaaaaaatat tatcattagg tctagcagta tcattaatct
    38341 tagtaaactt caaaagtgta aatgcatcat cagtagtaga aaaaatatac ggtaaagata
    38401 gatatgaaac agcagcaaag atagctgata aacagactta tgaaacagta attttagtaa
    38461 atacagaaaa atcacttgca gatggattaa gtgcaagtgg attatcggga gctacaaaag
    38521 ctccaatact atttacacaa caaaataaga taccagcaga cacaaataga tgtctaaaaa
    38581 atatcaaaaa agcatatata ataggtacag aagatactat aagtaaatca gtagaaaaag
    38641 aactagattc taaaaatata gaagtcaaaa gaattggtgg agaagataga ctaaaaacaa
    38701 gctatttaat agctaaagaa atagcaacta taaaaaaagt ggataaagta ctattaacta
    38761 atgcatatag tggagaagca gatgcaatga gtgtatcatc agtagctact agagatggag
    38821 ctccaatcat acttacagat ggaaagagtg tgccttttga tgtaaaaaat attcaatcat
    38881 attgtatagg ttcagaggag ataatgagta atcctctagt taaaaataca aactcagtaa
    38941 gaatagaagg aactgaccga tttgaaacta acaaaaacgt aatagactac ttttttaata
    39001 gtgcagatgg attctatgta tcagatggat accaattagt agatgcaata gcagctgcac
    39061 cacttactaa aaactctcca atggtactag taaatgatgg aagcgataaa actgtattag
    39121 aaggagctaa gaatataacc tctgtaggtg aaataaatga gaaggtaata caacagtgta
    39181 taaatgcttc aaattcaaat gggcaacccc caacaattac agttggaagt acagaggtat
    39241 ataagggtga aaagtttgac actggcaagt taaatatagt agctaaagat aacacaggga
    39301 aggtattacc aatagaagtt gatgggttta tagatacaaa tagagtaggt acatacatat
    39361 tgacattaaa agctacagac gaatggggaa aaagcacagg aaaaagagta gaaataaaag
    39421 tgttggatga caaatctcat gattacaaca gcccagaatt taaaaaaatg gtatctactg
    39481 aaatgtataa cttaatcaat tcctatagaa aagaaaaagg caaagagcct ttgctagtgt
    39541 ctagtaggct agaaggtatg gcaaatgcat ggtctaagta tatgatggat aaaaaagtat
    39601 ttgcacatta tatagatgga aaaaatgctc cacaagtatt ttctgaattt ggaatgagaa
    39661 gtgaggaaaa tatagcatat atttatatag attctaagaa cgttcaaacg acacaagatg
    39721 caaaagactt ggcaaaggct atatttgagg tgtggaagaa gtctccagag tacaatgcaa
    39781 acatgcttag taatgagttt tatagtactg gatttgggct ttatatatta tctgatggtc
    39841 aagtgcatgc tactcaggag tttttaaatg gaaatgaagg tagcttgtaa aaaattccat
    39901 aaatcttgtt tttttgttaa tttactgata ttataaaatg aaaatcacat ctaaagtact
    39961 ttttgtaatc tgttttgtta ttgctttaca ggctatggta ttttaggtgt tttttgtttt
    40021 agtagtttat agattttgag ggaatcacta ctgaggatgt aatttatgat tagctgaatt
    40081 atataaaaaa attagtagga ggagtttgat gaaggtaaat aagagagtat tatcaatagg
    40141 attgacaata tcattaataa tggcaggagc accaaatatc aatgcacttt cgagcataga
    40201 aaagatacag gggaaagata gatatgagac ttctgcattg atagcagata aacaaatata
    40261 tgatacaatc atattagtaa atactgataa ttctatagta gatggattaa gtgcaagtgg
    40321 attatctgga gttgcaaaag ctcctataat gttggtgcaa agagataaga taccaacaga
    40381 tgttgaaaaa agactaaagg atgttaagaa tgcttatgtt ataggtacag aggatacaat
    40441 aggtaaatct gtagaaaatc aacttaagaa taaagggatt gaagttaaaa gaattggtgg
    40501 ggaagataga ataaagacta gctatcttat agctaaggag atttcagcta ttaaaccagt
    40561 aaatgatggt gataaggttt ttcttgtaaa tggatacaca ggtgaggctg atgcaatgag
    40621 tgtatcttct gtagctgcta gagatggagt tcctgtgata cttactgatg gaaagagtat
    40681 accatttaaa gttgatggcg ttcagtgcta ttctcttggg tcagaggaga taatgagtaa
    40741 tgaacttgtg agtaagacta attcagttag gattgctggt aaggataggt ttgaaactaa
    40801 taagaaggtt atacaacgct tttataaagg aacaaagaag ttttatgttt ctcaagggta
    40861 taagttagtt gatgctgtgg ctggttctcc gttggctaag gacaaaccta tagtacttgt
    40921 taatgatgga agtgataaga gtgtgcttag aggtgctgat gaagttactt ctttaggtgg
    40981 aatggataag aaggttgttg accaatgtat tagttctgca tctgataaaa atacaatgcc
    41041 tacaattact gctaatgatg tagagatttc tgtaggggat aagtttgata atagtatgtt
    41101 gaatatagtt gctactgatt attatggaaa tgatttaaag gcaaatatta aaggaaatgt
    41161 tgatattaac aaggctggaa catatgtttt aaatataagt gtagtggata atttaggtca
    41221 gaagagtgag attagtgtaa atgttaaggt agttgtaaat gcgagtacaa aagactctaa
    41281 tagttatgag tttaaggcta tggtatctaa tgagatgtat gatttagtta attcatatag
    41341 aaaagaaaaa ggcaagaaga gtttaagaga gttagactct ttagctggaa tggcaaatgc
    41401 atggtctaaa tatatggaag ataagaaggt atttgcccat gagattgatg gtaaaaatgc
    41461 agctgaagta ttttttggct ttggagcaag aagtggtgaa aatattgcat atctaccaat
    41521 gaatgttaaa tctgtttata cttctaaaga tgcaaaagaa atggctgagt ctatttttga
    41581 tttatggaag aagtcttcta agtataatga aaatatgtta aaagaagaat tttatagctt
    41641 tggatttggt atgcatgttt ctagtaaagg agaagtgaat gcgacaatgg aatttttaaa
    41701 tagttagatt ttatattaat ttacattgga aaagatatag taaatttgta aaaataaata
    41761 tataaaatta aaataactta taaaattata aaaaataatt taaaaataat aaaaatgatg
    41821 aaaaaacaat taatttgttg gaaaaaactt caattaattt tgcataaatt attgaattta
    41881 tattttatat agtctatcat aagagtgtga gaaatatttg gattatatga acatggaaat
    41941 ttaatgtaca taattaaaga gatgtaatta atataatgtt gggaggaatt taagaaatga
    42001 ataagaaaaa tttagcaatg gcaatggcag ctgttactgt tgtaggttct gctgctccga
    42061 tatttgctga tactactgtt aaagaagaag gatatacggt agtacaagac aaatatgaaa
    42121 aattacttaa agaattaaag gcaaagataa aagatggaac tattacatca gttggtgttg
    42181 aatttgatgg aaaaccaatt actacattag caccaaaggc agatggtagt gataaagatg
    42241 caatagcaga acagttagaa actcttacta aaaaccaact taaaggttta ggtgatggaa
    42301 aatatgtaga ttttaaaatt acttatggtg ctaaagcagg agttccagct gcttcattaa
    42361 gtgcagatga tatacaaaaa tatgcagatc aaataaatgc ttctgagaaa atattagttg
    42421 aagtagcagc aggatctgaa gcaggtatag caaaatttga ttctgttaac aataaagtta
    42481 tagcaggaga tgctcctctt aaagttaaag atgcagtgaa agcgactgtt actactaatg
    42541 gtagtaataa aaaagttctt acaatatcag ctgcagctgg attatcagga ttttcatatg
    42601 gaacacttaa agacacaggt gcaagttcat ctgatgttga tgcaataact cttgacacta
    42661 caaatgcaac aataacagaa ggtgatacta aagtattaga ttttgataat tcatttaagt
    42721 ttaacgaaag tactaaaaaa gttggtagtt tagttacacc aaatacaaca aacactccag
    42781 cagatcctgg tactaaaact actgttagag tgataaaagc tgttgaaaaa acaatagatg
    42841 taagttcaaa ttcaactaca aaagctaaag atttagctaa acaatatgta ttcacagatg
    42901 ttagtgatac tgatccagaa agtttaagtt atatgcttaa aaacataaat gatggaaaag
    42961 ttgctgttaa aaattctgat ggagattatg aagtaactat attcccagaa ggaaaaagat
    43021 taaatacttt atctgcttct tcagctaaaa ctattttagg agataaagat actccagcaa
    43081 aaatagtttt aaaggcttca acaacaaaga aattagcaga ctatatagat gatttaataa
    43141 cttataacaa tagttattca aatgtacaaa ctgtagcagg tagtgataga atagagactg
    43201 ctatagagct aagccgtaag tactataact ctactgacaa aaatgcttta tatggagatc
    43261 ctgtaaataa cgttgtatta gtaggttctc aagctatagt tgatggatta gtagcatctc
    43321 ctttagcagc agaaaaagat gctcctttat tgttaagttc taaagataaa ttagattcat
    43381 caacaagagc tgagataaaa agagttatgg acttaaattc ttcaacagga ataaaaaata
    43441 ataaagaagt tttcatagca ggtggagtaa attctatatc taaagacgtt gaaaatgaac
    43501 taaaagatat gggacttaaa gtaactagat tatcaggtga tgatagatat gaaacttcat
    43561 tagcgatagc tgatgaaata gatataaatg ataaagctta tgtggtaggt ggaacaggat
    43621 tagcagatgc tatgagtatt gctccagttg catctcaaat aaaagatggt gaagctactc
    43681 caatagtagt tgtagatgga aaatctgaca agttatctaa agaagctgaa gatttcttag
    43741 atgatgcaca agtagatata atcggtggag aaaatagtgt ttctgctaaa atggaagatt
    43801 atatagatga tgctactgga aaatctccag aaagaattag tggtgctgat agacaagcaa
    43861 ctaatgctga agttataaaa gaatactttg ataaagatgg agtttcaaat tacttcttag
    43921 caaaagatgg ttcaactaaa gaggatcagt tagttgatgc attagcagca gcagcagtag
    43981 ctggaaacta cggttctaag cataatgaag atggagatat aactacagat gcttctcctg
    44041 ctccaattat tttagcaact gataatttat cagctgaaca acatgtagct gtaagtaaaa
    44101 ctgctactac taatggtgct aagaacttag tacaagtagg tcaaggaata gctgattcag
    44161 ttgttagcaa attaaaagat ttattagata tgtaatataa attttatata ttgaataaaa
    44221 aaggtttctc ttttgagaaa ccttttttat tttaaacata aatttttata aataaaaaga
    44281 ggctaattag cctcttttta atgtgagaaa cacttaaaaa ataagatgta attatttatt
    44341 taagtatcat tattatacta taccttaaaa taaatgtcta ctattctata attttaaggt
    44401 ataatagtaa gtggaaaata aataaggagg taaattaaat atgtcagttt tagatgtaat
    44461 attagacaaa acagatgaat tagaaattaa aaaattaaat aatatagtag ataaaataga
    44521 tgctttagaa gataaaatac aaatactaga tgatgaagca ttaaaaaata tgacgaatgt
    44581 atttaaggat aagttaagta aaggagaaac cttagatgac atattgccag aagcatttgc
    44641 tgttgtaaga gaagtatcaa aaagaatatt aggtatgcgt caatatagag ttcaattaat
    44701 tggtgggatt gtattacatc aagggaaaat agcagagatg agaactggag aaggaaagac
    44761 tctagtagga gtagctccag tttacttgaa tgcattgact ggtaaggggg tacatgtcat
    44821 cacggttaat gattacttgg ctcaacgtga taaagaactt atgcaaccag tatatgaatt
    44881 tttaggttta acagtaggag taattattaa tgggcaacag ccagtagaac gaaaattaca
    44941 atatcaatgt gatattactt atggtacaaa caatgagtat ggattcgatt atttaagaga
    45001 taatatggta acgcaaaaat cagagaaagt tcaaagagaa ctaaactttg ctatagttga
    45061 tgaggttgat tcaatactta tagatgaagc tagaacacca cttataattg ctggtggagg
    45121 agatgaaggt actaagctgt atgaacttgc aaatgctttt ataaagaata taagagatga
    45181 agattttgaa atggatagaa aagataaaac catagcactt actgcaagtg gtcttagtaa
    45241 agctgagaaa ttttttggaa tagaaaatat aactcatata gataatatag aaatttttca
    45301 ccatgtaaat caatctctaa gagcacataa attaatgaac atagatgttg aatatgttgt
    45361 tagagataat gagatactaa ttgtagatga atttacagga agaataatgg atggaagaag
    45421 gtttactgat gggcttcatc aagcaattga agctaaagag ggagtaaaaa taaatgatga
    45481 atcaaagacc atggcaacag taacatttca aaatttcttt agattatact ctaaattatc
    45541 aggaatgaca ggtacagcaa agacagagga aagtgagttt gaagctacat ataagattaa
    45601 tgttgttcag ataccaacta ataaaccagt tataagagct gatttacatg ataaagtatt
    45661 caaaacagaa cgtgcaaagt acaatgctgt tgtagaagaa ataataagga taaataaaac
    45721 taagcaacca gtgcttgtag gaactgtttc aattgaaaaa tctgagaaat tatctaagct
    45781 acttaaaaag aaaggtgtaa agcacgaggt cttaaatgct aaacaacatg ataaggaagc
    45841 tgaaataata tctaaagctg gtaaattagg ttctgttaca attgctacta atatggcagg
    45901 tagaggaact gatatatcac ttggagctgg agataaagaa gaagaagaaa aggtgaaaga
    45961 gttaggtggc ttgtatgtta taggtactga aagacatgag acaagaagaa ttgataatca
    46021 gcttagaggg cgttcgggaa gacaaggtga ccctggaact tctagattct ttgtaagttt
    46081 agaggatgaa gtaataaaat tgtatggtgg aaaaagtata gaaaaaatat taaagagaat
    46141 gaatgctaat gaagatgacg cgttagaaag taaggctttg aatagagcaa tagaaagagc
    46201 acagaaaggc attgaaggta aaaattttga acaaagaaag aatgttctta agtacgatga
    46261 tactattaat gagcaaagaa aagtaatata tgatgaaaga aataaggtat tggatggttc
    46321 tgatattgaa gaagagatac aaaaaatggt tatggatatt attacggagg ctgctgaaaa
    46381 gtacttaaaa aaatttagag actatcatgg atattttaag tatttatata atacttttat
    46441 gccagctgat actttattaa ttccagactt agataaaaaa aatgttgaag aaatagtaaa
    46501 tcaaacatat cagatttcaa agcgtgttta tgatcttaag aagatgatga ttggaattga
    46561 agaattggct caattagaga aacgagtatt acttcaagtt gtggatactt attgggttga
    46621 ccatatagat gcaatggatc aactaagaca atatataggt cttaaatctt atgcacaaaa
    46681 agatccattt aaagagtatg ctatagaagg ttatgaaatg tttgaagctt taaataggaa
    46741 tataagaata acaacagtgc aatatctata taaatttaac taaaaaagtg acccattggg
    46801 tcacttttta tatattaaga ttaaagtgaa atttatttta cagtacacac tcatatagat
    46861 aaatatttat aataatgaaa aagaggctga acgtcagcct cttttcctgt gtgagaaata
    46921 cggaaaaaag agatgcttga aatttctttc ttccatttaa tattatacta taaagagaat
    46981 aaaaagtcca tgagttatct ttatgattaa aaaaaaaaat tacaattttg ttacttttta
    47041 gtatacaata ataaatttaa attgactttg ttaattttta ttaataaaat atccaaatat
    47101 tacaatatct acagtttatt gaaataatgt gtaaattata ttataattgg taaataagga
    47161 aaaatggtga aaaccaaatt ttgtaggggg aaatatatga atagaaaaaa tctttatgta
    47221 attatggcag ctgcaatggt aagtacatca gtagctccaa gttttgctgc agaagctaca
    47281 caagtaaaga aacaaacaat aactaaaaag gaagcaacag aattagtttc taaagttaga
    47341 gaactaatgg ctcagagata tacaggaggt tctcaagttg gacaaccaat atatgaaata
    47401 aaagttggtg aaacttcatc acaattaaaa ataataacta atatagacga attagagaaa
    47461 ttagtaaatg ctttaggaga aaataaagag cttcttgtga ctatagcaga taaaggacat
    47521 acaacaaata gtgcaaatga agtggttgct gaagcaattg aaaaatatga aaattcagca
    47581 gacctttcag ctgaagctaa ttcaataaca gaaaaagcta aaactgaagc tactgggatt
    47641 tatggagtgg caactgtaga agcttcatat gacagtacaa aagataagtt agtaataact
    47701 ttaaaagata agactgatgt agtaacttct aaaacgctac aaataggtat tggtgatgaa
    47761 aaggttgatt taacagcaaa tccagttgat atagcaggaa ccaatttaga cccttctgca
    47821 gaaggattta gagtaagtaa aataaataaa ttaggtgtag aaggagctaa aaatattgct
    47881 gatattcaat tagctgaaat aactataaaa aatagtgatt taaatacagt ttcacctcaa
    47941 gatttatatg atggatatag attgactatt cgaggtaata caatagcaaa tagtgtagga
    48001 aaatcaatta gtgatatttc atcaaaagat tcagagacag gaaagtataa atttactgtt
    48061 aaatatactg atacatctgg aaaagcgata gaacttactg tagaaagtac taatgataaa
    48121 gagttaaaag atgttaaaga tgccttaaat ggaaatacaa aagtcaattt aatagctggg
    48181 gaagacaggt atgcaactgc tgtagctata gcaaaagaaa cagaatatac tgacaatata
    48241 gttatagtta attcaaataa attagttgat ggattagcag caacaccact agctcaatct
    48301 aaaaaagctc ctatattgtt ggcgtctgat aatgaaatac caaaagtaac tttagattat
    48361 ataaaggaaa taattaaaaa aagtccatca gctaaaatat atatagtagg tggagaatca
    48421 gcagtatcaa atacagctag aaagcaatta gaatcagtaa ctaagaatgt tgaaagattg
    48481 gctggagatg atagacatac aacttcagta gcagtagcaa aagctatggg ttcttttaaa
    48541 gaagcatttg tagtaggtgc aaaaggggaa gctgatgcta tgagtatagc tgctaaagct
    48601 gctgaactta aggctcctat aatagtaaat ggttggaatg atcttacagc agatgctatc
    48661 aagttgatgg atggaaaaga gattggtata gttggtggtt ctaataatgt acctagccaa
    48721 attgaaaatc aacttgctga tattgataaa gatagaaagg ttcaaagagt tgaaggtgaa
    48781 acaagacacg atactaatgc taaagttata gaaacatatt atggcaaatt agataaacta
    48841 tatatagcaa aagatggata tggaaataat ggtatgttag tagatgcatt ggcagcaggt
    48901 cctctagcag caggaaaagg accaattctt ttagctaaaa atgatataac aaactcacaa
    48961 aagaatactt tagataaaaa actaaatctt ggtgcagaag taactcaaat aggtaatggg
    49021 gttgaattga ctgtagttca aaaaataggc aaaatactag gatggtaata atcaataata
    49081 tagaaaagga ttccttatta tttataggaa tctttttcta tcgacataat attcattaaa
    49141 gtataacatg ctcttgacag ataggaggat atgatagtga tagaagcaac attgtttttt
    49201 tccccacacc aagatgatga aacattaagt atgggtagtg caataattga acatgtagaa
    49261 agaagcgata cacatgttat attatgtact gatggcagta aatcaataat aagaaaagta
    49321 ttagatgatg gagggtcatg ttcttatcat attaaagatg tacacaagta ctctttatca
    49381 gaaagtgatt tttcaaaaaa tagagatgaa gaatttaaaa atagttgtaa agcaatggga
    49441 gttaaagaaa ataatataca tatagaaaat aatagagctc atgatggaga attgagtaag
    49501 gaaaaagcaa gagatataat gctaaaatat ttagaaaaat atcctgatgc taaagtgaag
    49561 accgttacac catataaagc tcttggaata catgaagacc atagagcatt aggtgaagct
    49621 gcattagaat tgtatagaga aggaaaaata aaagatttaa gattttatgt agagccatac
    49681 gattataatg attttaaaaa agttaatcca aatgttagag tatggaaggt tttgagtagt
    49741 caagaagaga aattattaaa tgctatggat gcatacaaga aatggaatcc tgaaaatggt
    49801 aactacgcaa ttggatatca ttctgtaaaa tctcactttg atgaattaaa atccaataaa
    49861 atccaatatg tacatgctcc ataaacatta atataaaaac taggaggata tagttaatga
    49921 aaatatctaa gagaataatg tcgttgttga cagcaacttt ttttatcttt gcattatcta
    49981 tcaatatagc tgatgcttca tcaaaagaga gtctagtggg ctctggtaga tgggaaactg
    50041 caataaaaat tagtcagaat ggatggaaag atgctactaa tgctatacta gtaaatgata
    50101 attctatagc tgatgcatta tctgcaactc catttgctaa gttaaaagat gcacctattt
    50161 tattaactca aagtgataaa ttggatagta gaactaaagc agaattaaaa agactaggtg
    50221 taaaaaatgt atatttaatt ggaggtatga atactctaag caccaatatt gaaaaacaat
    50281 taaaatcaga aaatatgact tttgagagga tttcaggtaa tagtagatat gaaacatctt
    50341 taaaattagc tgaaagatta gataaagaga agagtatttc tgatatagtt gtagttaatg
    50401 gagagaaagg gctagcagat gcagtaagtg tgggttctgc tgcagcacaa gaaaatatgc
    50461 caattatact ttcagatcct aacaatggaa taaaagttgc aaatagcttt attagtagta
    50521 aaaatataaa gaaatcttat ataattggtg gtacatattc tgtatctagt tctattgaac
    50581 agagtttacc taatgtaaaa agattatctg gaaacaatag aaatgaaact aatgcaaagg
    50641 ttctagaaga attttataaa aatacagact taaaaaatgc ttatataact aaagatggta
    50701 tgaaaaaaca gggggatttg atagattctc tagctgtagg tgtattggca gctaaaaata
    50761 gctctccagt tgtattagta ggaaattcac ttgactctag ccaatatgat gtgttaaata
    50821 ctaaaagttt tgataatatt gtgcaagttg gtggaaatgg aaatgaagat gctgtaaagg
    50881 atattcttga tttacaagaa gaaactatat atagagttaa gacagcagac gaattggatg
    50941 ttgctctaag aaaagcagat gctaatgata ttataacatt taaacctgat gataataaaa
    51001 aaattactag ttcatttagt atagaaacaa agaaagctat aactgtagag cttgaaggat
    51061 tgtatacaca aacaataact gtaaatatgc caaatggaga attaactaat aagggtacat
    51121 tagatggaac tgtgaaaata atggatataa aaaatggcac atttatcaat gaaggtgata
    51181 taaatgatat tgacatttat gatccaaatg gatgtaaaat agagaataaa agtggtggag
    51241 atatatgggt tttaactata tctagtagtg ctaaagatgt aaatatagaa aataaaggtg
    51301 atataagtaa ggttacaaat aattctacta gcgttataat aagaaattca ggaaatattg
    51361 atactttaag tggaattaat gcaccagcaa taagtggaaa taaacctaga gttgatgata
    51421 ccgataagga tgaaaaaaga gcaagtggaa tatatcctac ttcagaacct ttatcacctg
    51481 caagagctgg ttatataagt gtaaaaatat tcactaagcc tgaagataca aattataaaa
    51541 tttattatag agtagtaagc agtagaccat ctgcattata tatcggagat agaatagata
    51601 caagtgactg gactgctgta ccatcttctg ttttaccatt tactattaaa gcagaaaatg
    51661 gatattatat agaagctgtt gaaataaatt ctgatactaa gaaagtatct agatggggga
    51721 aatctgacta tatagatgat ggattttatt taaaatcaga aactgcaaaa ggattaagtg
    51781 cagatataga ttttgatgga gattatgtta aattaacagc aacaggtgct gattctggat
    51841 gtacagttta ttatagaata gtatcaaaaa agccagtatc aatgaatgtt ggagataaag
    51901 taactatatc tgattgggat gaaattacta ctaatagagt gaggatagaa gcaaacgatg
    51961 taaaggataa atatatagaa ctagttgaat tggatgattc aactaataaa gtaactagat
    52021 ggggagcaag tgataaggta gtagaatagg tatctaatgt gaaacataaa aacagactga
    52081 ttaataatgg gcagtctgtt tttatttcct aaaaattatc attttactaa ataaataatt
    52141 taaaactata acaattatat tagtaaatac ttttacagta atatcattta caaaaaataa
    52201 atcaatcatt atatacataa taatcatttc tataatacca gaaagtagcc taaaactgac
    52261 aaaaaacaaa aactccttaa acaaaaatat tagttcaatt cttttactct caaacacaaa
    52321 aaacttatta gtaacataag caaataaaac agcaagtatc catgccaaag catttgcaac
    52381 cataaaatta aataaaataa ctctagtaaa aaataaatac gaaactatat ttacaagagt
    52441 agtaaatgct ccaaaaaata aatataatat cgtctctcta tgctttttta aaatcaaata
    52501 taatcctcct aagctaattt aatataaata tacttaaaga tactaacaaa cattagtata
    52561 acacatctat taataaatca atatatctta aaatattata aaaataaaac tataatattc
    52621 tataattaat atgtactcat aatccatatt ttatcaaaat aaaatatgac cttaatttag
    52681 aaatctgata taataaaaat taaatacata taagggggta aacatgagaa agtacaaatc
    52741 aaaaaacttg tcaaagctac tagcattgtt gacagtttgc tttttaatag tgtcaacaat
    52801 acctgtctca gcagaaaacc ataaaactct agatggagta gaaactgcag agtattctga
    52861 aagctatctt caatacctag aagatgtcaa aaatggagac acagcaaaat ataatggagt
    52921 aatacctttt ccacatgaaa tggaaggtac aacacttcga aataagggta gaagtagtct
    52981 tccatcagca tataaatcaa gtgtagcata caacccaatg aatcttggtc ttactacacc
    53041 agcaaaaaat caaggaagtc ttaatacatg ctggtctttt tcaggtatgt caacgttaga
    53101 agcatatctt aaattaaaag gatatggaac gtatgactta tcagaagaac atttaagatg
    53161 gtgggcaact ggtggaaaat atggatggaa cttagatgat atgtcaggga gttcaaatgt
    53221 aacagctata ggatatctga cagcatgggc aggtcctaag ttagaaaagg acataccata
    53281 taatcttaaa tctgaggcac aaggtgcaac taaaccttca aatatggata ctgcacctac
    53341 tcaatttaat gtaacagatg ttgttcgtct taataaagat aaggaaactg taaagaatgc
    53401 tataatgcaa tatggttcag tgacatcagg ttatgcacat tattcaacat attttaataa
    53461 agatgaaaca gcatataact gtacaaataa gagagctcca ttaaatcacg ctgtagcgat
    53521 agtaggatgg gatgataatt attcaaaaga taattttgca tctgatgtaa aaccagaatc
    53581 aaatggagca tggttagtaa aaagtagttg gggagagttt aattctatga aaggattctt
    53641 ctggatttct tatgaagata aaactctact aacagataca gataactatg caatgaaatc
    53701 agtatcaaaa ccagatagtg ataaaaaaat gtaccaactt gaatatgctg gtcttagcaa
    53761 gataatgtca aataaagtaa cagcagcaaa tgtatttgat tttagcagag actctgaaaa
    53821 acttgattct gttatgtttg aaacagactc tgtaggagca aaatatgaag tatattatgc
    53881 accagtggta aatggagttc ctcaaaacaa ttcaatgaca aaacttgcaa gtggaacagt
    53941 atcatattct ggatacataa atgtacctac taattcttac agcttaccaa aaggtaaagg
    54001 agcaatagta gtagttatag acaacacagc aaatcctaat agagaaaaat caactttagc
    54061 atatgaaact aatatagatg catattattt atatgaggct aaagcaaact taggtgaaag
    54121 ctacatactt caaaacaata agtttgagga tataaataca tatagtgaat tttctccttg
    54181 taactttgtt ataaaagcta taacaaaaac atcttctgga caagctactt caggagaatc
    54241 tttaactgga gcagatagat atgaaacagc agttaaagtt agtcaaaaag gatggacttc
    54301 ttcacaaaat gcagtattag taaatggaga tgcaatagtg gatgctttaa cagctacacc
    54361 atttacagca gcaattgact ctccaattct tttgacagga aaagataact tagattcaaa
    54421 aactaaggca gagttacaaa gattaggaac taaaaaagtt tatctaatag gtggagaaaa
    54481 ttctttaagc aagaatgtac aaactcaact tagtaatatg ggtatatcag tagaaagaat
    54541 ttcaggtagt gatagataca agaccagtat atctctagct caaaagttaa atagtataaa
    54601 atctgtttca caagttgcag tggcaaatgg tgtaaatgga cttgcagatg caataagtgt
    54661 tggtgcagca gctgctgata acaatatgcc aataatactt actaatgaaa aaagtgagtt
    54721 gcaaggtgct gatgagtttt taaattcatc taaaataact aagtcttata taattggtgg
    54781 aacagctact ttatcatcaa atttagaaag taagctttca aatccaacaa gacttgcagg
    54841 cagtaataga aatgaaacta atgctaagat aatagataaa ttctacccta gctcagattt
    54901 gaaatatgct tttgttgtta aagatggttc aaagagtcaa ggagacttga tagatggact
    54961 agcagtagga gcattgggtg ctaaaactga ttcaccagta gttctagttg gaaataagtt
    55021 agatgaaagt caaaaaaatg tacttaagtc taagaaaata gaaactccta ttagagttgg
    55081 tggaaatgga aatgaaaatg cttttaatga actaaatact cttttaggaa aatagtgaat
    55141 ggaaagatag taaggatata cattttatta ttaaaaaatg ttcaatagaa gtttataaat
    55201 ataaaataac aaaaaggggt gtctcaaaat gaacttttta gttcatgagg cactcttttt
    55261 tgttgtttta tatttttatc atttaaataa tgaaaaatag gattgtctat atttttagat
    55321 agttttctta ttattgatga aaaatgtaat aattttaaac tgtaaaatag tgtaaagtta
    55381 ttttataata taaatatagt aagattttaa acaaaataag ggggaataat aatgaaagca
    55441 ccaaaaacta ttttaacaat actgacaata gcgcttactt taagtagcat ttctataata
    55501 ccatcatatg cacttacaga ggaaaaatta ataggtaatg gtagatatga aactgctgta
    55561 aagataagtc aaaaggcata cagttctagt cctaatgtag tgctagttaa tgacaattca
    55621 ttagctgatg cactttcagc tacaccattt gcaaaggcta aaggagcacc aatacttttg
    55681 acagaaagtg ataagctaga tgataggact gaaaaagaga taaaacgtct aggagctaaa
    55741 gatatttatt tgataggagg tactgctgta cttaataaag atatagagaa caagttaaaa
    55801 agtaatggac ttaatgtaga aagaattaat ggtaaggata gatatgaaac ttcactgatt
    55861 ttggctaata agcttaagga tataaaagat attaaggaag ttgctgtagt aaatggtgaa
    55921 aaaggattga gtgatgctgt tagcgttggg gcaccagcag ctcaaaataa gatgcctata
    55981 attttgtcta gtcctaagga tggagtagaa gcatttgata aatttataag ggatgagaaa
    56041 gttataaaag catatgtaat tggaggtaca aattctgttt caagagctgt agaaaagagt
    56101 cttcctaatg cagaaagagt gagtgggaaa gatagaaatg agactaatgc taaagttata
    56161 gaaaagtttt acactgatac aaatttaagt aatttatatg taactaagga tggtagtaaa
    56221 aatgagaatc aactaataga ctcgttagct gttggcgtat tggctgcaaa aaatgagtct
    56281 cctgttgttt tggttggtaa taaattaaac acaaaacaaa gagatatact tagtactaaa
    56341 aaattaaata ctataacaca ggtaggtgga aatggaaatg aagaagcttt tgatgaaatt
    56401 aaaagtctac aagaaaaaac tgtgtttgaa gcaaaaactg ttgaggaatt aactgatatg
    56461 ataaatatag caagtcctaa tgatattata aactttaaac caaaagagaa tactgtaaat
    56521 gaagctttta gaatggtaac aaataaacca ataactgtga atataaaggg tgattgttca
    56581 aagactctta cagtagatat gcctaatggt gaagtgaata actatgctac tttggtaaat
    56641 gttatagtta gaaatattgg agaaggtgga tttaataatc atgatacaat cactatattg
    56701 tcagttcgtg acaaaaatgg tagagttata gaaaatacta gaaattcaga tatagatacg
    56761 ttgatgatat tagcttctgc taatgataca aaattgatta atgatggata tataggaaaa
    56821 cttatagata attcatctaa tagtgatata actaataatg gtactataga taaaaaagtt
    56881 aatcaggttg aagatttaga ggctaaagta gattcaattg aaaaagctat agattctata
    56941 agtcaaaaag tcaataaaat acaagatata ctagataagc ttggatttct aaagaaattt
    57001 cttagctaag aatatgtata tttaagtgaa gtgtgtgttg taattgttct aaagttatgt
    57061 agttaaacag gtttatatac aaatagattt atagataaat atgtagttta gtatatattt
    57121 gaataagctt tggggaggta acatggggat tggaattgaa tgtaacaaaa aaataaataa
    57181 aagtattgac aaaactagaa taaagaaaaa taatctaaat agcaatagca atagcaatag
    57241 caatagcaat agcaatagca atagcaatag caatagcaat agcaatagca atagcaatag
    57301 caatagcaat agcaatagca atagcaatag caatagcaat aaaatttcaa ataatgctgt
    57361 agattctaaa cttatgaata gtaggattaa atcaatagat ataataagag ggctatcaat
    57421 agccctcatg atagtctgca ataatcctgg gacttggatg agaatgtatc ctcaattaag
    57481 acatgctgta tggcatggtg taacacttgc agattttgca tttccttttt ttgttatatc
    57541 tttaggtgtt acaattccaa tatcaataaa tagtaaacta aaaaataata aatccacact
    57601 gagtataata cttagtatat tcaagagaag tatcttattg atattatttg gatttttctt
    57661 aaattaccta ggaaatcctg atttagatac tgtaagaata cttggagtac ttcaacgtat
    57721 gggattagtc tattttgtga ctagtttagc atatttactg ctaaaaaaat taaatgttgg
    57781 aagtactgct acaataatta cttttctatg tatagctaca tttataatag ttgggtacta
    57841 catcttagca aaaccttatg gatttgagct agaaggttct cttgcacagt tagtggattt
    57901 acatttcttc aaaggacatt tgtacaagcc ggaatttgaa ccagatggtt ttttaactag
    57961 tatagtagca atttcttcag gtatgttggg atgcactatg ggatgtgtac ttttaaaaga
    58021 aaacctagga gaatataaaa aattctttaa aatactggtt atgagcataa tcctgctaat
    58081 aggtgctttt atctttaatc agtattttcc atttaataaa agattatggt ctagctcatt
    58141 tgtactttta atggctggca gttatggtat attactgtca atattttatt ttatatgcga
    58201 catcaaaaat aaaagtaaaa tatttacccc aataatagca cttggaagta gccccatttt
    58261 tacatatatg tgccttgaga tattgagtca tgttttttgg aatgttccaa aacttacgaa
    58321 caaagtcgat tatccaacta ccttggtaga gtggactaca tatgaactta taacaccttg
    58381 ggcaggtaca acttgggatt ctcttatatt ttctttattg tatgtcctat tctgggttat
    58441 agtaatgtct ataatgtaca agaaaaaaat atttattaaa atttagtgca atatttttag
    58501 atgataaagt ctttgatttg ttcaaatata cagaaactat caaatataat caaaatttct
    58561 cctcgataaa atttaaaaac catgccataa aagattacaa aaagtttaca atttgtaatt
    58621 ttttgtgcta taatgaaaaa agatttacat aatattgaat ttatgtaata taaaacaagc
    58681 ttaagatgta ttaaaaaaga tttaaagatt taaagaacca aaaaattagg ggggatatga
    58741 tgaaaaaaac aacaaaatta ttggcaacag gaatgttgtc tgtagcaatg gtggcaccaa
    58801 atgtagcttt agcggctgaa aatactactg caaatacaga atctaattca gatattaata
    58861 taaatttaca aagaaaaagt gtagtgttag gaagtaaatc taatgcaagt gtaaaattta
    58921 aagaaaaatt aaatgctgat tcaataacat taaattttat gtgttatgat atgccacttg
    58981 aagctacttt aaattacaat gaaaagacag attcatatga aggtgtaata aactataaca
    59041 aagacccaga atacttaaat gtatgggaac ttcaaagcat aaaaataaat ggtaaagatg
    59101 aacagaaagt tttaaacaaa gaagacttag agtctatggg attaaatctt aaggattatg
    59161 atgttacaca agagtttata ataagtgatg caaattcaac aaaagcagta aatgaatata
    59221 tgagaaaaac ttctgcacca gtaaaaaaac ttgctggagc tactagattt gaaactgctg
    59281 ttgagataag taaacaaggt tggaaagatg ggtctagtaa ggttgtaata gttaatggag
    59341 aattagcagc agatggaatt acagctactc cacttgcatc tacatatgac gcaccaatat
    59401 tacttgcaaa taaagatgat ataccagaaa gtacaaaagc agagttaaaa cgtctaaatc
    59461 caagtgatgt aataattata ggtgatgatg gttcagtttc acaaaaggca gtgtctcaaa
    59521 ttaaatctgc tgtaaatgta aatgtgactc gtattggtgg agttgataga catgaaacat
    59581 ctttacttat agcaaaagaa atagataagt atcatgatgt taataaaata tacatagcaa
    59641 atggatatgc tggtgaatat gatgctctta atatttcatc aaaagctggt gaagaccaac
    59701 aacctataat attagctaat aaagattctg taccacaagg tacatataat tggttatctt
    59761 cacaaggttt agaagaagct tattacatag gtggtagtca aagtttatca tcaaaaatta
    59821 tagaccaaat atctaaaata gcaaaaaatg gtacttcaaa aaatagagtt tctggagcag
    59881 atagacatga aacaaatgca aatgtaatta agactttcta tccagataaa gagttgagtg
    59941 caatgttagt tgcaaaatct gacataatag ttgattcaat aacagcagga cctttagcag
    60001 ctaaattaaa agctccaata cttataactc caaaaactta tgtttctgca taccataata
    60061 caaatttatc agaaaaaaca gcagaaacag tatatcaaat tggtgatgga atgaaagata
    60121 gtgttataaa ttctatagca tctagtttat caaaacacaa tgcaccaaca gaaccagata
    60181 attcaggttc agcagcagga aaaactgtag tgatagaccc aggtcatggg ggttctgatt
    60241 caggggcaac aagtggatta aatggtggag cacaagaaaa gaaatatact cttaatacag
    60301 cattagcaac tactgaatat ttacgttcaa aaggtataaa tgttgttatg actagagata
    60361 cagataaaac aatggcttta ggagaaagaa cagcattatc aaatactata aagccagatt
    60421 tatttacaag tatacattac aatgcgtcta atggagctgg taatggtgtg gaaatttatt
    60481 acaaagtaaa agataaaaat ggtggaacaa ctaagactgc agcatcaaat atcttaaaaa
    60541 gaatactaga aaaatttaac atgaaaaata gaggaatcaa gacaagaaca cttgataatg
    60601 gaaaagatta cctatatgtg ttaagaaata acaattatcc agcaatactt gttgaatgtg
    60661 catttattga caataagagc gatatggata agttaaatac tgcagaaaaa gtaaaaacaa
    60721 tgggaactca aattggaata ggaatagaag atacagtaaa ataaaataga aaatattaaa
    60781 gcaaaacata tcatctcctg ttataatgta aaatataaca agagatgata tatttttgta
    60841 aagaatcaaa ttgtttacaa tgatgtttct tgtattgata atctgctcat tattgagtat
    60901 aatatagaaa taagtcaaaa ataagataga aataagttga aattaagaat taaaaaaatt
    60961 tttattccaa tatggaggta actatgtctg gatatactaa tgatgagtgt gaaataccta
    61021 aaattaaaag ctatccaggg gcagataaag aaattgcaag tgagattgac tatagcatag
    61081 taaaaggaac agttttattc gacttgtacc aaagaattat ggatttagtt ttatctataa
    61141 taggacttgt gataggttta cctttaatag caatatttgg aattcttatt aaaatagagg
    61201 acaaaggacc aataacttat aagcaagaaa gactaggaaa atgcgggaga agattctata
    61261 tatataaatt gagatctatg agaacagacg cagaaaaatt tggcgcacaa tgggctgaaa
    61321 aagatgatcc tagaataact aaggttggaa agtttattag gaaaactaga attgatgaga
    61381 ttccacagct gtttaatata ttaaaggggg acatgggctt aataggtcct agaccagaga
    61441 gaccgaactt tactgttcaa tttaatgagg aaatacctgg atttatcaac aggctcgcta
    61501 taaaacctgg gcttacaggt tgggcacaag taaatggtgg atacgaaatc actcctgaag
    61561 aaaaacttaa agaagatatt tactatataa aaaataggag tatattactt gactttaaga
    61621 tactctttaa aactgttaaa gtagtattga caggagacgg agctagataa ttttaaaata
    61681 ttagattact aatcaatgaa aaagctcttt aaaaagagct tttttattgt caaaaaatac
    61741 aaaaaagaaa aggaatgaga acttatgatt aaaaaaattt ctactatatt atcgttagtt
    61801 ttgttaatct ctatttctag tacgatagga gtatttgcag atgcaaatcc caaaagagaa
    61861 ttgatagaag ggagtatacc agagatttcg acagaattaa ataaaagagc atttaaggat
    61921 tctaaagaag taattcttgt aaatgaagag tcaattgtag atagtataag tgcaacacct
    61981 ttagcatatg caaaaaatgc tccaatagtt gtaactaaga gtaaaaatct tggtagagta
    62041 acaagaaatt atctaaaaga actaggtcca gagaaggtta cgatagtagg tggtttaaag
    62101 gcagtatcta aagatgctga acgtaatata gaaaaaatgg gaatgaaagt agaaagaata
    62161 agagggaaag acagatatga tacatcttta aagatagcta gagaaatgta tagaacagta
    62221 ggatttgatg aagcattttt acttagctca acaacaggat tagagaacgc catatctgta
    62281 tattcttatg ccgctaaaag tggaatgcca ataatatggg ctaaagatga agggtttgag
    62341 gaacaaattg atttcttaaa aggcaaaaat cttaaaaaaa tatatgcatt aggtgattca
    62401 aaagagttta ttgcagagat tgattctaac ttaaaaaata tagaaggaat aaagcaaata
    62461 aataagtcaa gtacaaatgt tgatttaata aagaagtttt atgatgaaaa agatataaag
    62521 aaaatctata ctgctagatt agactttggt agtagatctg atgtaaacga atatatttct
    62581 ttgggagtag tatcagcaaa agaaaatatg ccaatactga tttgtagtga taacttaagt
    62641 cgtgcacaag ataaattctt gaaagatagc aatataaatg atgtagttga agttgggtat
    62701 acagtaggtg attatagtct atttaagagc atatttaatc taacattctt atcttgtata
    62761 gtattaatac tattattact attgattaca tttagggcac ttagatatga gtcgaaatag
    62821 tttttgattt ataaggaagc ttttcatagg ttaaatttac ttaattagaa taatgtttat
    62881 aagcagtttt aaaagttcta cttttagagt ttaaattaga taaggagaaa gaagatgtca
    62941 aaaacagcta aagcagcatt gtggattatg gcagccacta tgttttcaaa agtattaggt
    63001 ttccttagag aacttgtact tgcaaatttc tatgggacag gtatgtatgc agacgtattt
    63061 gtattaacat taaatatacc tggtttaatc atagcagtca taggctctgc agttgctaca
    63121 acttatatac ctatgtactt tgagacaaag aaaagactcg gagatgaagg tgccttaaaa
    63181 tttactaata atgttttaaa tatatgctat ataatggcta tagtaatagc tattattggt
    63241 cttttattta cagagcaatt tgttacagta tttgcagcag gatttagaaa cgaccctgct
    63301 aagttccaag cagcaatatt atttactaaa ataatgattt caggagtttt attcctgagt
    63361 ggaagtaaga tatttagttc gtttttacag gtgaatgaca gttttgtaat tcctgggctt
    63421 ataggaatcc catataatat tataataata gcagcaatag cactaagtgc aggtaaaaat
    63481 gtatggatac tgccagcagg agcattattg gctatggcga gtcagctatt gtttcaactg
    63541 ccatttgcgt ttaagaagtc ctacaagtat aagccatata taaatttaaa ggatgagtct
    63601 ataaaagagt tagtcaacct agtattgcct atgttggtag gagtagctgt tggacagtta
    63661 aatatatttg ttgaccgatt attggcaacc actttgggtg atggaaagtt gtctgctctt
    63721 aactatgcaa ataggctaaa tgagtttgtt atggcattat ttgttacatc tataataaca
    63781 gttatatatc ctaaactagc aaaaatgtct ggtaaagata ataaagaagg atttataagc
    63841 actattgtaa aatcttcaaa ttgtattata ttggtggtgc ttcctatatc tataggagct
    63901 attatattag cagagcctct tgttagaata ttattccaaa ggggtaaatt tgatgcgtta
    63961 tcaacagacc ttacatctat agcgcttaga ttatattcac taggtttatt ggcttgtgga
    64021 gtaagagatg tcttatatag ggcattttac tctctttcag atacaaaaac accaatgata
    64081 aatggaagta tagctttaat aataaatata gttcttaatt taatattgat aagaccattg
    64141 ggtcatgcag gtattgctat atcaactagt acatcaaaca taattacagt tatattatta
    64201 tttatatctc ttaaaaagaa aaatggatat tttggagggg ataaaattat aaagactggt
    64261 ctaaaaagtt tggtagcatc tggtgtgatg gcagtagcta ctttattgat atataataat
    64321 ctatatgcat ttatgggttc aggaacaatt aaagaaatta tatcagttgg agctggagta
    64381 ttaggtggag cttctgtata tactgtattg atagttttat ttaaggtaga agagatggat
    64441 ttagcttttg aatttttaag aaaaggaaaa caaaaactac ttagaagata gataattacc
    64501 ttgaaatttt aaattagagt cagaaaggat gaaaatatgg attataagaa taattatgag
    64561 atgtggttaa attctcctta ttttgatgaa caaacgaaaa atgagctatt aagtataaaa
    64621 gatgatgaaa aagaaataca agatagattt tataaaaatt tagagtttgg aacaggtggt
    64681 ttaagaggta taataggagc gggaactaat agaatcaata tttatactgt tagacgtgcg
    64741 acactgggcg ttttaaatta tatcatgaaa actcaaggag aagaaggtaa gcaaaaggga
    64801 atagttatag cacatgatag tagatacatg tctagggagt tttgtataga agtagcaaag
    64861 actttaagtg cttatggtgt aaaggcttat atttttgaag aattaaagcc tacaccagaa
    64921 ctttcttttg ctgttagata tttaaagtgt gctatgggaa tagtaataac agcaagtcat
    64981 aatcctaaag aatacaatgg atataaagta tatgactcag atggtggtca aatctgtata
    65041 gatatggcaa atgatataat agcagaagtt aataagattg atgattacag tactattaaa
    65101 agtatagatt ttgaagaagc tttatctaaa aatttaataa ctatattaga taatgaagta
    65161 gatgatgagt ttataaaagc tgttaagaaa caagttttaa gacaaaatat aatagatgaa
    65221 tacgggaaaa aattaaaaat aatatataca cctattcatg gtacaggaaa taagccagta
    65281 agaaaagtct taaatgaatg tggttttgaa aatgtaatgg tagttaagga gcaagaattg
    65341 ccagattcta atttttcaac agtaaagtat cctaatcctg aagaaaagtc agtgtttaat
    65401 atagctattg aaatggcaaa gaataatggc actgatttga taataggaac agaccctgat
    65461 tgtgatagag taggtatcgt tgtaaaagat tcaagtggtg aatatgtagt cttaaatgga
    65521 aatcaagtcg gttcactttt agttagatat atattagaga gtttagttga agaaaataaa
    65581 cttcctaaaa acaatcctac aataataaaa actatagtta catcagaact aggtgcaaaa
    65641 atagcaaagg cttataatgt agattgttta aatactttaa caggatttaa atttatagga
    65701 gaaaaaataa aagcgtttga agaaagcaat gatagaagtt ttataatggg atatgaggaa
    65761 agctatggtt acttaattgg aactcatgct agagataagg atggtgttgt atcttcactt
    65821 atgatatgtg aaatggcagc atactattct tcaaaaggta tgaacttgta tgaagctttg
    65881 atagatacat ataataaatt tggatactac aaagaggact taaagtcagt aactttaaaa
    65941 ggcatagatg gaataaaaaa aataaaagag atgatgcttt actttagaag tgttaaaata
    66001 gataatgtgg ctgatgtaaa agtagataaa attttagact ataaagatgg tgttgatgat
    66061 ttacctaagt cagatgtact taaattttta cttgaagatg gctcttggat agctataaga
    66121 ccttcaggta cagaacctaa aattaaattt tactttggag caaattcaga caatcaagaa
    66181 gatgtagaat ttaagttgaa caatttaata tcttatatat taaatgtagt ggattctata
    66241 taaaaagcta aggggagata aacaatgaaa gtatacaatg tcataatggc tggaggagga
    66301 ggaactcgtt tctggccact tagtagacaa gaagtaccta aacagcttat aaatttaagt
    66361 ggagaagatg ctttaataaa cgaaactata aacagaatag attctctagc aaaaaaagat
    66421 gatttattta tagtaaccaa tgagaagcaa ttggaagctt taaaggatat agtaaaagat
    66481 aaatgtttgg atagtaatat attgccagag ccatgtgcca gaaatacagc agcagctata
    66541 ggctttgcag catttaacat aatgaaaaaa tatggtgatg gtgttatgtg tgtatatcca
    66601 gctgaccatt atataaaaga tgaaaaagaa tttaaatcta ttttggaaaa agctatctat
    66661 atagcagaaa ataatgataa actagtaact attgggataa ctcctacatt tccatcaaca
    66721 ggatatggat acataaattt taatagagaa aatactatag aagatgttgc atatgaggta
    66781 gtggagtttg tagaaaaacc aaactatgaa attgctaaag aatatgtaaa ctctaaaaag
    66841 tatgtatgga atagtggaat gttcgtatgg aaggtatcta aaatactaga agattttaag
    66901 agatacttgc caaaagtata tgaaaaatta gaagatataa gtaaatactt aggaacaaaa
    66961 gaagaaatgg aaaaaataaa agaaatatac ccaactatac aatcaatctc tatagattat
    67021 ggtattatgg aaagaagtaa tgatgttata gtagttcctg gtgattttgg atggaatgat
    67081 gttggttctt gggattctct aggtgcaata tatccaactg atgatgaagg taatatcaaa
    67141 agaggtgaaa atattactat agatactaaa aattcaatta tatattcaga tgacaaatta
    67201 atatcaacaa taggtattag tgatttgata gtagtatcta caaatgatgc agttatggtt
    67261 tgtagaaaag ataaagctca agatgtcaaa aaaatagtag agcagttaaa agaagaagat
    67321 agacaagaat atatgtagga ggtgttcatt tgattcctaa aaaaatacat tatgtatggt
    67381 ttggtggtcc aaaaggaaat atagaaaata tatgtataaa ttcttggaaa gaaaagcttc
    67441 cagaatatga gatagtagag tggaatgaaa aaaactttga tatagagaaa gaaataaaag
    67501 gaaataagtt tttggaagaa tgttataaaa gaaaactttg ggcattcata tctgattata
    67561 caagaataaa agtactctat gaacaaggtg gagtttatat ggatacagat atgcaaatat
    67621 taaaggatat aactcctctt ttagaaaata ataggctagt ctgtgggtat gaagatgata
    67681 gagagtatat aaatggtgct ataataggtg tagaaaaagg acatcctttt ttaaaagact
    67741 tattggagta ttatgaaaaa gaagtactta cttcttcatt gtttacaata ccaaagataa
    67801 tgactcatct tatggaaaag aattataaga agattgatcc aaataattat gaagaaggaa
    67861 tacgtgttta cgacaaagaa tatttttatc cttttggatt taaagaagac tttacaccag
    67921 aatgtataac agaaaacact tttgggatac attggtgggg aaaaagttgg gctaaaaaga
    67981 gaaattattt cttagaaagc aaacacttaa caggtgtaaa taagatatgg aagtgttgta
    68041 aaatatttgc aagtaatact ttacgaagct aaaagggaga aagtaaaatg aagactttaa
    68101 atgatgatag aaatattatg attagaaaat atctttatat atttgttttg tttatcatat
    68161 tacttagaaa gtatatgata tcatttattg acccaaatat ggatataggg atgattaaat
    68221 cagcgttatt ttattcttca ataggaatac taatgctact ttttttattt gataagagaa
    68281 aaagtataac agaaatggta ctagttggag tttgtgtctt actttattta ttaaataggg
    68341 aaggagctat attattaatc gttttattag ctgtatcagc taagcaaata gatgataaat
    68401 ttatagttaa aaattattta ataatatcgg catgtttttt aatggtagct atattattat
    68461 ttaatttatt tccttcatta atatttaatc aggaagtcca ctatagatat atagaaaaaa
    68521 tagatatgct tgttacaaga atggattttg gacttggtaa tccaaatagt gtattttatc
    68581 atatggttac tatatatgca gcatatatat tcttaagata taaagattat aataaatggg
    68641 atagaatact tttatttggt tctgcatttt ttgtttatca aaccacatat agtagaactg
    68701 gatttttcac tatattagca ggattaatat ttgtagaaat aataagatgg atagatataa
    68761 aaaaaataaa aggactacca atgctcctaa agacattacc ggtaatactt actttattta
    68821 gtgtaattat tggtacagta tttgataaaa gcactttatt aaatagatta ttagcaagta
    68881 gacctaagtt ctggaatgta tacttggctg aaaaggggaa ctttctaaaa ccttttggaa
    68941 attcatatag tcctgctata aaagcaacta atccattaga tagttcttat gtatatataa
    69001 ttagtgtact aggagtagtt gcttgtatat tatttatgta tttgatgtat aaaggaatag
    69061 aaagctttat agaaaaagat aaaaaagcat atttagtagc tttatttata tttttactat
    69121 actcttttgc agaaaattta ttattagaag cttcatttag ttttgcagta gtattactaa
    69181 taaaagaagt gatattaaat gataaaaggg aaatagattt gtggaagatg aaaagtagga
    69241 ggtagtaatg ttaatatcat taataatgcc aacattaaat agatatgatg atatatactt
    69301 acttatggac agcttagaaa atcaaactta caaaaacttt gaacttatag tagtagacca
    69361 aaatgataat agcaaagtta aagaaatagt tgataaatac attgataagt tagatataaa
    69421 gtatataaaa agctctaaaa aaggattgag ttataacaga aatgtaggca tagacaatgc
    69481 tgtagggcaa attataggtt tccctgatga tgattgtgtc tacgaaaatg atacattaga
    69541 aaaagttata aatttcttta ataaaaataa agattataaa atatacagtt gcaaaactat
    69601 ggattcaaat aaagttgatg catttaagaa aatgtatgat ggaacttgtg atatcacaag
    69661 ttccaacgtt ttggatacta taacatccat aacttttttt attgattttg aaggtaaaga
    69721 ctatacaaga tttgatgaaa aattgggtgt tggtggagag tttggtgctg gtgaagagat
    69781 agattatgtt ttaaacttat tgagtttagg ttttaaagga aaatactttg gaaatgatat
    69841 aatttaccat cctgcaaaaa aacattctaa atctaaagaa aagtatcaaa aagattataa
    69901 ctatggaaga ggctttggag cgctttgcaa aaaggaaata gtctatagaa aaaattataa
    69961 gtttgcaaaa gttatggtat caaaacttgt aagaaatata ggaggtctta tattgagttc
    70021 caatagagat tatcatagtg ccactataaa aggaagaatt aatggattta gacagtacaa
    70081 gttataatga ggatggtgtt tatggacaag gttaaatttt taataaatat gcttttggct
    70141 ttttttatat atccatttaa taagcataag tttaatggta gagatatctg gcttataggt
    70201 ggacattcag gggatatata caatgataat tcaaagtttt tttatgagta tatgctaaaa
    70261 gaacataatg atgtagaaac ttattgggta gtaaataaag acagtaaagt atttgataaa
    70321 attccaggca aaaaattaat aagaggaagt gtagaaaact atctgtatta ttacaattca
    70381 aaagctatag tattttcaca tgcaccatca gcagatatag caccatacaa ttttgctgta
    70441 cctgttctta attattttca taaaaaaacg ataaaagtgt ttttaaatca tgggactata
    70501 agttttaaaa aaagaaaacc tatgaacaaa aagttcaaaa atattataga taacctatat
    70561 aaaagctata atatagttac agcaagttct gaatttgaaa gaaatgttat ggtaaatgat
    70621 tggggaatgt tagatgactc agtttatata attggaaatg caagatatga caatttacca
    70681 acaaatgaag ttgcacaaac tcgagatata ttatatactc ctacatggag agattggata
    70741 aaatttagtt caggaaaatt tacggataca gattatttta aaaatataat gaatttttta
    70801 aatgatgaca aattaaataa aatattagat gagaaagata taaatgtaaa aatttatatg
    70861 catcatctta tgcatgagtt tatagatgat ataaaagaaa atataacagg aaaacgaatt
    70921 gtgtttttag ataaaggggt aactctagca aatgaaatca gaaaatcagc tgcaaatata
    70981 actgattatt caagtgtagc catagacttt ttgtatatga atagacctat cttgttttac
    71041 caattcgatt tggatgaata tatggaaaaa gttgattcat atatagattt aaaaagtgaa
    71101 atgtttgggt ctttggctta taataatgat gaagccgtga ataaacttat tgatattatt
    71161 gaaaataatt ttgaggttat ggataaccaa aaaaatgaaa gaaataagtt tttcagatat
    71221 aatgataata agaactgcaa gaggatatat gattgtgtat taagtaaaat taaataaata
    71281 ttttataaat gatgaggaag gtaatatgaa gaagaattta gtatctataa ttactcccat
    71341 gtataattct gaaaaattta ttgaagcaac cataaaatca gtattaaacc aaacttatca
    71401 agaatgggaa atgttaatta ttgatgattg ctcaacagat aatagtccta atatagtcaa
    71461 atcttatatg caacaggata gtagaataaa atgtataaag actgagacta ataagggtgt
    71521 ctctaatgct agaaatttag cactaagtaa ggcaacagga caatttatag cttttttaga
    71581 tagtgatgac caatggaata gtagtaagtt agaaaaacaa gtaaatttta tgttagaaaa
    71641 tgactatgta atttcattta cttcatatga actgatggat gaaaatgata aaaaattaaa
    71701 caaagtaata aaagttccac ctaatgtaga ttatagaaga ttattaaaag gaaatatctt
    71761 aggatgtctt actgttgtga ttgataagtc taaattggat tttgaaataa gaatgagtgg
    71821 tgtaagacat gaagattatg ttttatggtt atcgatattg aaaaaagggc atattgctca
    71881 tggaataaat gaagttctag cattatatag aaaatctagc aattccctaa gtggtaataa
    71941 aataaaagct gctatgtgga cttggaatat atatagaaat atagaaaaaa tacctttata
    72001 caaagctata tactacttta ttaattatgg aataaacgga atcaaaaaaa gttagacaga
    72061 gaggaagatt taattaatgt ataaatataa atttactgta tttactccga cgtataacag
    72121 agctcattta cttgaaaatt tgtacaatga tttaaaagct caaacttatg atttcaatga
    72181 ttttgaatgg ttgattgttg atgatggaag tagcgacagt actaaagagt tagttgaaaa
    72241 atttatatca gaaaataaat taaatataag atatatatac aaagaaaatg gaggaaaaca
    72301 tactgcaata aatctaggtg taaaaaatgc agatggagag ctatttttca ttgtagatag
    72361 tgatgatggt ttaattaaag attctcttga aattgctaat gatgagtggg attctttaga
    72421 gaataaagaa ggatttagtg gagttgttgg actttgtgta tatccaagtg gagatttgat
    72481 agggacagaa atgcctgaag ataagaaaat atgtcacttt gcagacctat attttaagta
    72541 tggcgttaag ggagataaaa gcatagtatt tgtaactgat gagcttataa aatttccttt
    72601 tccagaaaga aaagaagtga gatttttacc agaaagtgta gtatggaatg aaatgtctaa
    72661 atactataaa gttatatgtg taaataaacc tatgataata agagattatt tggatgatgg
    72721 tttgactaag aatatattaa gtaaaaatgc attaagaggt agagcattag agtttttata
    72781 tttaataaat caaaatacat atccattaag tagatatcca tatatgtgga taaaaaatta
    72841 cataaattta gccagatact cattgttgtc tgatagtcat tattttggtg aattaagaaa
    72901 agtatctgat aaattgttgt atttagcatt attcccatta ggatattaca aatatattgg
    72961 acaaagaaaa ttagtatcta agtaaacttg ttggtatagt actgctagtt gctatataag
    73021 gagagtaaaa atatgaatga gcctttagta tcgataatta ctcctgttta taattcagaa
    73081 gagtttttat cagagacgat aaagtcaatc caaaatcaaa cttataaaaa ttggcagctt
    73141 ttattagtgg atgattgttc taaggacaat tcaagtgaaa ttataaaaag ttttcgaaaa
    73201 gaagatgcta gaataaaata tataaagctt gaaaaaaatt ctggagctgc agtatctaga
    73261 aatgttggta ttaagaatgc ggaaggtcga tttattgcat ttgtagatag tgatgattta
    73321 tgggatagta gaaaattaga gattcagata gaatatatgt taaaagaaaa tgtaggtttt
    73381 tcttttacat cttatagata tatgagacaa gatgggtcaa aaacgaataa ggttgcaaga
    73441 gctcctaaga aaattgacta tgaaggatta cttagaaata ctataatagg atgttctaca
    73501 gtggtcattg ataaggagat tgtaggagag ttcagcatgc ctttggtaag aagaggacaa
    73561 gatactgcaa cctggttaca gttacttaaa aaggaaaaat atgcatatgg gattcaagaa
    73621 gatttagtga attacagatt agttggaaat tctatatcaa gtaacaagat aaaagcatta
    73681 aaacgaactt ggaatactta tagaaatgtt gaaaatttaa gtttaccaaa aagtctatat
    73741 gtattttgtt tctatgtttt taatgccatt aaaaaaagag tataagtttt atatacttgg
    73801 aggtaaaaaa atgaaaatag ctatagctgg gacgggatat gtaggtttgg ttacaggtgt
    73861 ttgtctagca gaggtaggca atgaaaatgt agtctgtgtt gatgtagatg aacaaaaagt
    73921 taaatcaatg aaacttggaa tagcacctat atatgaagaa ggcctagaag aacttatggt
    73981 taaaaattac aacagaggaa gaatagatta cactacagac tataaaaaag cttatgaaga
    74041 tgtagatgta atattaatag ctgtaggtac acctgagaga agtgatggtt cagccaacct
    74101 agattatata aaggtagttg caaagcaaat agctgaaact gtgacaaaag atactctagt
    74161 agtaataaaa tctacagttc caattggtac aaatgaagag atagaagatt atataaatag
    74221 aaacctaaaa aatgatgtaa aagtagaact tgcatctaat ccagagtttt tatcacaagg
    74281 aacagcagta aaagatactc tacatgcttc aagaatcgtt ataggtgtag aaagtgaatg
    74341 ggctagagaa atattagagg aaatctataa gccttttaat caacctataa tagttacaaa
    74401 tagaaatagt gctgagatga taaaatacgc atcaaataac ttcttagctc ttaaaatatc
    74461 atttatgaat gatattgcaa atctttgtga aatagcagga gctaacatag aagatgtagc
    74521 tttaggaatg agttatgatg atagaatagg aagtaagttt ttaaaggcgg gaataggata
    74581 tggtggttct tgctttccaa aagacacaaa agctcttcac tggttatctg aacataatgg
    74641 ttatgaatta aaaacagtaa aagctgcaat agaagttaat gaaagtcaaa aacttaaatt
    74701 agttagaaag gcatctaagt tagtagagag ctttcaagat atgaaggttg ctgttttagg
    74761 tttgactttt aagccagaca ctgatgatat aagagaagca ccatctatac caaatataaa
    74821 atatttgctc gaaagagggg caagagttac tgtatacgac ccaattggtg taaataatac
    74881 taagaaaata tttggtgact ctatacttta tgctgattct gtagatgatg cgatagaaga
    74941 tgcagagata tgttttataa tgacagaatg gaaagatgta ttaaattatg atatacataa
    75001 gtataaggaa aagatgaaaa atcctttagt ccttgatgga agaaattgtt ataaaattaa
    75061 agatatggaa gagttaggaa ttgagtatta ttcgatagga aggtaaatat tatgaagact
    75121 gtttgttatt tagctgatgg gtcaaatcct catactttaa aatggtgtag tttttttaaa
    75181 aataaaggat atgacattca tgtaatctct ttaaatggag gtcacatgga aggaataaaa
    75241 atttataatt ttggttctaa tgtagaagaa ctaaaaaatg aaaatatttc taaaaaaacg
    75301 ggatacttag gttcaataaa gcagataaag aagctagtaa atgaaataaa gccagatata
    75361 ttgcatgcac agtatgcaag tagttatgga tttataggaa gtctacttgg gtatcatcca
    75421 tatattattt ctgtttgggg tactgatatt tatgatttcc ctaataatgg ttttatacaa
    75481 aaacaaatta taaagcataa ctttagaaaa gcagactata tcttttcaaa tagtagagat
    75541 atggctaaag aggctaataa atatacagct aaacatgtag atgtaacctt ttttggagta
    75601 gatatggata ggtttaagcc tatggaagta gaaaaagaag acgcttttgt aataggaatt
    75661 ataaagagtt tagaaaaaaa atatggaata gaatatttaa tacaagcatt taagatgctt
    75721 aaagatgaat ataaagataa gaaaataatt cttaaaatag gtggctctgg aagtcagatg
    75781 gacaacctaa taaatttagc taaagaattg ggtatagaaa atgatgttca gtttttagga
    75841 agaatatctc cagaaaatgt aagcaagact tttaattctt ttgatgtaac tgtatttcct
    75901 tctttaagag agggatttgg agtggcagct atagaatcag aagcatgtga agtacctgtt
    75961 atagttacaa atgtaggagg acatccagaa tctgtatggg aaaatgaaac tggcttaatt
    76021 gtagaaccaa aacaaccaga agaaataaaa aatgctataa taaaactaat ggaaaatgat
    76081 gaacttagac ttaatatggg taagaaaggg cgtcaatttg ttagagaaaa ttatgaggtt
    76141 aatttaaact ttaatgatat agaaaaaatt tatgattcta tatttgataa atataaaaaa
    76201 taaaatgaaa aaatacagca aaagatagat gttgttggct atgcttaggt gcgtagcttt
    76261 tgctgtttaa aggagggctt ctagttatgc gagaaagtat agatttagag aaagaaagct
    76321 tttttaatag gctttttaaa aaatataaat ttagacattt aacactatta attctagata
    76381 tttgtagcgt actagtagcg tttcgaatgt ctttaagctt gacaggtaat ttaaatgcat
    76441 ttaccactaa tgatataata atttcagtgt gtatatatat agtacttcat atagtatcat
    76501 ttagattgtt taagtgctac aacacattat ggagatatgc aggagaagaa gaaattatat
    76561 ctatttttgt ggcaacttta gcttatttaa ttccgattta tataataaat aaattattag
    76621 ggtttgatta tcctataatg ttttatgtat taaatacaat atttattata atgtttacaa
    76681 gtggcgcaag aatagcctat agagctataa gaatagtcat gaacaagaca tattctagag
    76741 gaaaggtttc aaatatcctt ataattggtg caggagatgc aggagaaatg gttatacagg
    76801 aactaaaaag aaatccagaa cttaagaaag tagcagtggc tataattgat gatgataaaa
    76861 ataaaattgg aagaataatc cataatgtga aaattgtagg aacaacttca gatataaaag
    76921 atgtggtaga aaagcacaat gtagatgaga taatcttttc aatagcaaac atagagaaac
    76981 gtagaaaaaa agagattatt gatgtatgta aaaacactaa ctgtaaaata aagacaatac
    77041 caggaattta cgaaattata gatggaaaag ttgatataaa acagattaga gaagtagaga
    77101 tagaagactt gttgggtaga gagcctataa aaacaaattt aagagagata agtaattaca
    77161 ttgagggaaa agtaatccta ataactggag gaggaggctc aattggttca gagctttgta
    77221 gacagattgc aggatttaat cctaaagagc ttattatagt tgataattat gaaaataatg
    77281 cttactcaat acaacaagaa ttacttagaa aatataaaaa taaattagat ttaaagacag
    77341 taatagcatc aataagagaa gaaaaaagaa tggatgaaat atttaataaa tataaaccag
    77401 aagtggtatt tcatgcagca gcacataaac atgttcctct tatggaatcg agccctggcg
    77461 aagctataaa gaataatata tttggaactt taaacatagc tggcttgtct tcaaaatata
    77521 gagctaagaa atttgtcctt atatctacag ataaggctgt aaaccctact aacataatgg
    77581 gagctactaa aagagctgct gaaatgatta tacagactat gaatgctgaa agccagactg
    77641 aatttgttgc tgttagattt ggaaatgtgc ttggtagtaa tggtagtgta attccacttt
    77701 ttaagaagca aattgaagat ggcggaccag tgacagttac tcatccagat attattagat
    77761 attttatgac aataccagaa gcagtaggct tagtcataca agctggggct atggctaaag
    77821 gtggagaaat atttgtactt gatatgggag aacctgtaag aatattggat ttagcaaaaa
    77881 acttaattaa attcagtgga tttgagccag atgttgatat aaaaattgaa ttttctggtc
    77941 ttagaccagg agaaaagtta tatgaagaac tacttatgtc agaagaaggt ctgttggata
    78001 cagagcataa aaagattttt attggtagac caatagatgt tgatagagaa aaaataacaa
    78061 aatacttaaa acttttaaga gaaattacta acaatgaaga ggttgaaaaa atagatggta
    78121 ttatgaggga attagtgcca acttatatta aaccagaaga tgctaatatc aaagaaattg
    78181 caacaactag agaaaagtag aatatatttc taatttgtaa ccagggggag caaaaagaaa
    78241 caaatatata ctaaaaagaa acaaaagatt acaaaaagat tacaaaaaga ttacaaaaaa
    78301 ggttgcaatt tattttgatg taagatagaa tgttaattag gaattggaaa cgaaggaaca
    78361 aaatgttaaa ttttcgaaag gattaggtat ttgagattat gatgaatatt aaaaataaaa
    78421 aacatatatt gaaaaaattt atagctatgg tgttaatagc aggagttgtc acagtagaag
    78481 caggagcaat aacggctagt gcagcagagc caactaattc acctatgagt gcaacagtag
    78541 accagtgtga ttttttaaat gtaagaagtg gagctagtgc aaatgatgca gtagtaggaa
    78601 agataaacac aggagacaaa gttgaagttt tagaattaca ttcaaatgga tggattaaaa
    78661 taaaaagtgt tgacaatgtt acaggatggg taaatggaga ttatttaact attcaaggtg
    78721 gaaatgtaga tgcaaaggta caaaatgttt taaacttagc atttaagcaa caaggtaagc
    78781 catatagatg gggagcaact ggaccaaatg cttttgattg ttctgggttc acttcttatg
    78841 tctataaaaa tggagcagga gttaatttac caagagtatc aagatcacaa gcaacagtag
    78901 gaaagaaagt atcaagagca gaattaaaac caggagattt agttttcttt ggttcaggtg
    78961 gttcaataaa tcacgttgga ttgtatgtag gagattctaa attcatacat tcaccacaaa
    79021 caggagatgt agtaaaagtt actagcatgg ctccaggaac taattatgca agaagactta
    79081 taactgctac acgtgtttta caataataat agatattgat ttacatagga ttgaatatta
    79141 acattaaata tataattaca tttaatgttt tacatagaga tttaagagtt tagtaatatt
    79201 aaaatagtga tgaacgttat aaaaacagca aagaagataa atttctttgc tgtttttttt
    79261 ataataaatt atttcatatt ataatcaaat atcaaatata aattttaact atattattca
    79321 atatttgaca gataatatga attttagata tacttaataa gaagtatatt gtagaaaaaa
    79381 taaatttaaa aaattaaggg ggatgaaatg aaaaaaataa gtatattagt attatcattg
    79441 ataatgacac ttactatgtg tagtgtaagt tcatttgctg attcatcaaa tgataaagaa
    79501 atgagagctg cttggatatc tacagtttat aatttagact ggcctaagac taagaataat
    79561 gaagccaagc aaaaaaagga atacacagat ttattggaca aattaaaaag tgtaggaata
    79621 aatacagcag tggttcaagt aagaccaaaa tcagatgctt tatataaatc aaatataaac
    79681 ccatggtctg aatatcttac cggtacacaa ggtaaagacc ctggatatga cccattacca
    79741 tttttaatag aagaagcaca taaaagagga atggaatttc atgcatggtt taatccatat
    79801 agaataacta tggcagatga gagcatagat aaattgccag caaaccatcc agcaaagaaa
    79861 aatccaagct gggtagttaa acatggaaat aagtattact acgacccagg tttaccagaa
    79921 gttagaaagt acatagtgga tagtatagca gaagtagttc aaaattacga tatagatgga
    79981 gttcattttg atgattactt ctatccagga gtaagtttca atgatactgc aacataccaa
    80041 aaatatggaa aaggacaaaa taaggatgat tggagaagag aaaatgtaaa tactctactt
    80101 agagatgtaa aagcgtctat aaagagtata aagcctaatg ttgtatttgg tgtaagccca
    80161 gcaggtatat ggagaaataa gagtagtgac ccaacaggtt cagatacatc aggtaatgaa
    80221 agttatgtag gtacatatgc agatacaaga gcatggataa aacaaggatt gattgactat
    80281 gtagttccac aactttactg gccaattggt cttaaagcag cagattattc taaattagtg
    80341 gcatggtggg ctaatgaagt aaaaggaaca aatgtagact tatacatagg acaaggtata
    80401 tataagcaag gtcaatcgag ttatggtgga caaaatatag caaaagaaat agtacaacaa
    80461 gtaacattaa atagaaagta tagtgaaata aaaggtagta tgtatttctc agctaaagat
    80521 atagcaaata gtacttctat acaaaaggat ttaaagagtt tatattctag tagtgaagaa
    80581 ccagttactc caccatctaa tgttaaagtg gagaaattaa gaggcgacga aagatatgac
    80641 acagcagtag caataagtaa aaaaggttgg gctacaaatt cagatacagt agtattagta
    80701 aatgggtact ctatagttga tggtataaca tcaacaccac ttgctacatc aaatgatgct
    80761 cctatactat tggtaaataa ggataatata cctacatcca ctaagaatga gttaaaaaga
    80821 cttaatccaa gtaaagttat tttaattgga ggaaataact ctattggaga taaagttgag
    80881 agtgagataa aagatacatt gtcaaatgta tcaataaata gagttggtgg ttcagataga
    80941 tatagtacat ctttaatgat agcaaaagaa ttagtaaaaa ctaatccagt agaaaaacta
    81001 tatataacaa gtggtacagg tgaagcagat tctctttcta tagcttcaaa agcaggagaa
    81061 gaaaagcaac caatagtatt agtatcaaaa gataatgtaa gtgatgaagt ttacaattgg
    81121 atatcagatt taaaagtaaa agatgcttat tttataggtg gtaatctaag tatatctgat
    81181 tcagtaataa ataagctgga taaggtcatt acaaatgatg tatctaaaaa tagaatagct
    81241 ggagaaaata gagaagaaac aaatggaaaa gtaatacaaa aattctatcc aaatgctgaa
    81301 tattcatcaa tgtttgtaag taaaagtaat caattggtag atgcattaac atcaggtcct
    81361 ttagcggcta aattaaaatc accagtagtt atgcttggaa atagtgtaac atctgctcag
    81421 aaaactgctt tagagcctaa gaaaactact ctagtatacg aagctggaga tggtataaat
    81481 caaaacacat taaacacttt cttaaactta gttaagtaat aaaaaagtta atttaaaata
    81541 cagagtattt tacaaatgta gattaaaatg aaaattatca tgataaatta gaaaataaaa
    81601 aagaactata tgcattttgt aatcaagtgt acacagcaac ttgaaaataa ttatgcgata
    81661 tagttctttt ttagtaatta atcttatact ttttaataaa ctttatgttg aattttttat
    81721 tgtttaaact ctatgtcatt aaatataaaa tcgtgtaata tgtctaaagt atctgggtca
    81781 aatctaagaa cccatccagc ttttccatat ataccacctg tactatgaat ttcatcatca
    81841 ataggaaatt caaattgctt aatatttaag tcacccattt ttaaaacttg agctcctaaa
    81901 gaaagtattg cattaggctt catgttagtt ttaacatatg gaagaactgc atctaataaa
    81961 cctggatatt tagtaacagg taaagtttcc gttttcttga ttatagcttg tataatctgt
    82021 ctctgtctgc catctctagc aaaggctgta tcgttgtgtc tgatacgtgc aaaagatagt
    82081 gcctgatatc catttagagt ctgctttcca gagtttctaa tgtattgtat gctaccttta
    82141 tcatcacttt tactccattt ataagtttca ggaataaatt tatttagttc tcgtatttct
    82201 cctttttcaa cagtaacctc aacgccacca atagcatcaa ttatatacat aaatgattcg
    82261 aaattaacac aagcataatt ttgaatatca atattaaaat tctcttcaat tgtttgtatt
    82321 aataaatcag cttgtccata agcataagca tgagtaagtt tttgtttacc atgaccaggg
    82381 atgtctacat agctatcacg agctaatgat gtaagtttga tatcattgtg tttgttatca
    82441 atagtaagta tcatcatagc atctgacctt gaagaatctt catttggtct agcatctgtt
    82501 cccataagta gtatgttaat tatcccatct tcatttttat ggtcattgtt atccaataaa
    82561 tcactactta tgctcgagtc atgaatagca gataatttat aataaaaata tccatagaca
    82621 cttataggga atattactac caagaaagcc agtagtataa caaatttctt taattttgac
    82681 aaaagtaccc cttctttctt ctttttcctg tatatttttt aatagcatac acttaattat
    82741 attaaagtat tgagatatat tcaatacttg tttcagttac aaaaaaaaga caatgatata
    82801 atttataaga aaatttatcg acctatatat atagttaaaa gtatatataa tatatttttt
    82861 aaaagtaatt tatattttaa aataaaaaca caccccataa atacaaagta atttgcattt
    82921 aagaggtatg tatttatcat caaattattg gtttgttttg gaatttctaa tcttcaacca
    82981 taatatcttt aaatatgaaa tcatgcattg catctagttc atattcctca aaaggtatta
    83041 catagcctgc attacctatt tttctaccat tttcagtaga aagaggaaac tccatagatt
    83101 tcaaatttaa atttcctata cttaaaagtt cttttcctaa agatattatt tcattaggat
    83161 tcatattagt ttttacatac ggaagtattg tgttaactag attaggatat ttagtgactg
    83221 gtaaatcctt aacaccattt attaaactct gtataacact tctttgtctt ctatctcttt
    83281 catctgtact atcatttttt cttatacgag aaaatgcaag tgtctgatat ccatttaatt
    83341 tttgctcacc agaatttctt atatatttaa gagaaccttt attgtcattt ttattccatt
    83401 tataagtttc aggaataaat ttatttaatt ctcttatttc actcttatca acagtaacag
    83461 taactccacc taaagcatca actatatcca taaaagagaa aaagtcaact acggcgtagt
    83521 tttgtatatc aagttggaag ttttcttcta tagtttctat taataagtta gcttgtccat
    83581 atacgtaggc atgtgttaat ttttgttctc catgtcctgg tatattaaca taagtatctc
    83641 tatttaatga agttagctta agacttttat ttttattatc aatagtaagt atcatcatgg
    83701 catcagaacg agaaacttct tctcctggtc ttgcatctgt acctaacaat aatatgtttg
    83761 tgatattttc ttcgttttta tagtcattac tactaagcgc ctctgtattt atactatcat
    83821 cttgtatagt ttttaattta gtaaaaacat atccatatat actaacagga aatgctataa
    83881 ctaaaagggc tagaaatata acgaactttt tgagtcctga caataatatc ccctactttc
    83941 taaatttttt aattacacat agttaattat attaaaaatt tgttacataa gcaataaaaa
    84001 acacaataaa ttagcataag tttgtcataa tataattatt tttatagcat tttatagaag
    84061 aaagtgtgta gttattattg attttattgg aatataattg tatgaagact taaattttta
    84121 taaactaatg tgtatattga aaaaaatatt ttcctgaatg tagttatagc atcttatgaa
    84181 aggtgcaaat tgtaatttaa aatttttagt gtataataag aattgaaaat tcaaaccgat
    84241 aaaaattaat aaaaaataat tataaattaa taatttgggg gaggaaatgt atgataaaat
    84301 taatagcaac agatttagat ggaactttgt tagatgaaaa gagcgaaata aatccagagt
    84361 tttataaagt atttaaaaaa ttaagagaga gagggataat gttttctgct gctagtggca
    84421 ggcagtatca aaatctgata aaaaaatttg aagatataaa agatgatatg atgtttatat
    84481 cagaaaatgg aacactagta gtatataaag gaaaagaaat attatcaaat cctttaaata
    84541 aagaattagt aaatgaaatc atagagacaa caagaagtat aaaagggaaa aaaattgtaa
    84601 tgagtgggaa aaaatacgct tatatagaaa gcaaagatga agcgtttata caagaggtta
    84661 gtacttatta tgcaaagttt aaaattgtag aagatttgac gaaagtagag ggagacatat
    84721 tgaagatagc tgtgtttgac ttcaaaggtg cagaacataa taataatatc tactttgaaa
    84781 agttttctga tagagctcaa gtatgtattt ctggagtgga gtggcttgat ttaactgcaa
    84841 aaggtgcaaa taaagggagt gccataaaaa aagtacaaaa aatgttagat ataaaatatg
    84901 aagaaactat ggtatttggt gaccagttaa atgatgtgga aatgatgaaa agtgcctatc
    84961 atagttatgc catggaaaat gcaaatgaac atttgaaaca aattgctaga tttagagcaa
    85021 aaagaaatac tgaaaatggc gttgtagata agataaaaga ggttataaaa attggatagt
    85081 catatgtata gatgaaaaag attattttga agaaatcaaa aaaatatcaa tgtaattttg
    85141 ataaataagt ttatttatgc gaaaattaca ttgatattta ataaacatca ataatagata
    85201 tttatttttt tatgatattc agtattttgt tttctatttg agttgcattt tcagcagaaa
    85261 ttaaataaac atacctacct gccttctcaa ttatagtatt atttgctatt tcagaattga
    85321 ttttttctcc atgtccttct ccaaaaggtg cttgtatcat atctttttta taattttcta
    85381 gagttttata gattttatct acctcgtcag tttttataac gataatatct tctagagata
    85441 atttttcaga agaattaatt acaaaacctt gaattatgtc ttcctctaca tcattaaaat
    85501 agttaaaatc ctttgcatca aagatttttg attcctttat aagtaaatca gaagatttta
    85561 atgctttttt tatttttgat gtagaaacgt ttttgtaatc accacttgaa gaacaactaa
    85621 ccattcctaa tgataagatt atcgaaaaaa acaatattaa aatttttttc atgataaacc
    85681 tccaagtttt ttaaactctg tcttaaatta gtttactaca taattatata aaaataaatg
    85741 tatttaatgt tacagttttg attaataatg taatattttg ataataatac ctattgagag
    85801 gtgattagaa atgagaatac caaaacatgt aggaattata ccggatggaa atagaaggtg
    85861 ggcaagtcaa aatggtatga caaaagataa aggatatgaa agtggaatag accctggact
    85921 agagtcttat agattatgta gagatttagg ctgtgaagag gtgacttttt atggatttac
    85981 aactgataat acaagacgtc catcagtgca gacaaaagct tttacaaaag catgtattga
    86041 tgctgcaaat atattaatag atgaaggtgc tgctttatta gttgtaggaa atacaaaatc
    86101 gccaatgttt ccagaagaat tattaccata tactaataga attaaagcca aagaaggtca
    86161 aataaaagca aatcttctgg taaattatgg ttggtattgg gacttaaata atatatcagg
    86221 agtagaaaat gtaaataaaa ataatataca ggattacata cagtcatttg atgtatccag
    86281 actagacttg ataatcagat ggggtggaag aaggagatta agtggtttcc ttcctgtaca
    86341 atcaatttat gcagattttt atgttataga taattattgg ccagatttta agagtgacga
    86401 tataaaggac gcatttagtt ggtatgatac acaagatgtc actttaggtg gataattaaa
    86461 aatattctga tttaaggttg tagagtgttt taaattattg aaaaaaaaac ttcttgatgg
    86521 taaaataaca ctaataatat gtaaaagtgt aaaaatttat accaagaagg gtgaagaaat
    86581 gtcaaaaggc aataataaca ataattcaag aaacaaatct aaaaagacca gtcatctgaa
    86641 tagaaaaaag cgaaaattaa ataagaaaaa gttagcagtg ttgatttgct ttacagtttt
    86701 atttttattt attgcattta aggctactca aggtgtagta gctttggtaa aaagtatgga
    86761 taaaagtaat aaaactagtc aacaacaaaa tgttaactca gaacaatttg accttggtaa
    86821 tgaagaggaa aataaaaaga aaaaatatac tgtatttata gacccaggtc atggaggcaa
    86881 tgataaagga actgaaagca agaccagtaa tagatatgaa aaagacctaa atctacaaat
    86941 tgcaaaaaaa ttagcaaata aattatctaa acaaaaggat attcaagtag tagtatccag
    87001 aactgatgat acatatatat cattaaagga tagagctatc ttagcaaata atagttctgc
    87061 tgatgtgctt gtttctattc atttaaatgc agagaaaaat ggtaatacag caacaggtat
    87121 agaaacttgg tataggaata aagctacaga tggttcaaaa gaacttgcac agacagtaca
    87181 aagtactata gtatcctatg ttaaagttag agatagggga atagttgaaa ataactttga
    87241 agtacttaga gaatcaaata tgcctgctat attaatagag tgtggttttt taacaacacc
    87301 ttcagaggaa caaaaaataa taaatgaaaa atatcaagac caacttgcgg aaggaattgt
    87361 tcaaggtgta ctttcatacc tagattctaa agggaataag taaattaact ttactttaat
    87421 gtgaggataa atttaaaagt ttaactgtgt aaattgtatt aactttattt cgtaatgttg
    87481 gaaatattgt ttgaatttag aaactatgaa attagaaatt attttatcta tatctaattt
//
LOCUS       DAEPIN010000009        74849 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.009, whole genome shotgun sequence.
ACCESSION   DAEPIN010000009 DAEPIN010000000
VERSION     DAEPIN010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 74849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 74849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..74849
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.009"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            <1..901
                     /locus_tag="KR250_000755"
     CDS             <1..901
                     /locus_tag="KR250_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBH0066720.1"
                     /translation="KNIYKSFGDVFIYNSKLDYIYKLYGDLYIKFGNIDEGISCYIKG
                     LNLVKKSSLNLRKISIFYGLIANGYELKKDFKLAIEYYKSMDWYSDRWEKNNSYKTSQ
                     AIHKQYELRQKQESLRLLVDDNKKLENDILKDGLTKLYNRRYLEQQLDLFNETRDKNM
                     VAILVDIDCFKSYNDNYGHLAGDKVIVKVTDIIRSVFIDITEHIIRYGGEEILILVDS
                     IESIGVEEIDNIELYIKRVFKLLEFEGIEHIYSKIKNYITISAGVSIKRCRSRKDVEI
                     LIEDADKNLYKSKKAGRNQYIII"
     gene            909..2909
                     /locus_tag="KR250_000756"
     CDS             909..2909
                     /locus_tag="KR250_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBH0066721.1"
                     /translation="MILLRKKIGIIASDIELKERIEELYREDVENGTIIIDILNLDLM
                     ENQGRILVEKGAQAIIGRGGGYSLVIDTVNVPVIPMNMKSTDLLRAIEIAKKYSKKVV
                     LILGDNEVSFDYVGWRNVISTEITEEWFESKYEIRSKVVKYIDQKDEIVIVGGGLACS
                     FARQYGIDSVFATASDESIREAVEYCKKLLDTLGEEKFNNEVLRNILDGIKDGVIAID
                     SNGSIILYNESAKNMLKVERKCALNKYILDVFPKMEWMLDCLHEKEDVEDRKIRNINN
                     LIVNTRTTLIKVDNSTYGVLGIIQDITKLQNLERKIRFDLNQKGLYARYTFDDFLFKD
                     KLTKEFIEEAKKIGKSDYTTLLYGESGSGKEIIAHSIHNISKRKDRPFVAINCATIAE
                     NLLESELFGYEEGAFTGARKGGKRGLFELAHGGTLFLDEINSLSFNIQTKLLRVIEER
                     QIMRIGSDYIIPLDIRIIAATNESLTEKIVMGTFRADLFYRLSSLEINIPPLRDRRED
                     IIPLFNNFVNEVLKDDGLNGINSIDENFVLTKDEMDKLYNYSWPGNVRELKTIAQKYV
                     VTGKIKLRQDRDFKTKKLLPNSEVDKFNSETTASVEVQDESINISKINDGKISIDIKE
                     VNKYVEEKIISMLFAQGLSKNEVAQVLGISRTSLWKKYNKNI"
     gene            3171..4430
                     /locus_tag="KR250_000757"
     CDS             3171..4430
                     /locus_tag="KR250_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBH0066722.1"
                     /translation="MSQSMVGKHAMWPKENDVIFSISGRAQAAEKAFGMDNVINATIG
                     ALMDDSGKLITMKTVYEEYKALDNCEIGAYAALEGQPDYLEAVKKVFFRDYLPEGHIR
                     VLASPGGSGAIKLAVWNYTNEGDEVLTSDWFWSPYVSIAEEANRKVVNYQLFDENRRF
                     NFESFKEKFVNIAEKQGRVFTIINTPAHNPTGYSVADDEWDKILDLSKEVAKDKDKKI
                     IFFVDSAYIDFAGDDDVCRKFFKKFSNLPENVLVLVGFSMSKGFTAYGMRMGAIICIS
                     SSEDVAEEFHYSCVHSCRANWSNCNRSAMAVLSNIVNDPKKFKEYEDEKEIYKNMLTR
                     RADVFVKEAERVGLEILPYIAGFFVSIPCDNPKEVCEELTKHNLFAVPLKMGLRFAVC
                     AVSEDKCKKAPSIIKEALESLEVKINN"
     gene            4491..6278
                     /gene="iorA"
                     /locus_tag="KR250_000758"
     CDS             4491..6278
                     /gene="iorA"
                     /locus_tag="KR250_000758"
                     /EC_number="1.2.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HBH0066723.1"
                     /translation="MKQLMTGNEAIARGAYEAGVKYASAYPGTPSTEILENIATYKDA
                     IVAEWAPNEKVALEAAIGGSIAGARTMASMKHVGLNVAADPIFTYAYTGVNGGMVLIT
                     ADEPGMHSSQNEQDNRMYAKFAKIPLFEPSTSQEAKDMIKEAFEVSEKYDTPVLYRVT
                     TRLCHSKGLVECYDREEVEIKEYVKNAKKMVTVPANAQIRRGVVEERMEILKKFSNET
                     DLNYYEINDTKIGVIASGMCCNFAKEVFGKNASYMKLGFTNPLPYEKIKEFAEKVDKI
                     YVIEENDPFIEEQIKAYGIDCIGKDVIPPYGEMTPDVLRKAIFGKTNDTIEYKSELVT
                     PRPPSFCAGCPHRGFFYELGKRKNLIVGGDIGCYTLGFAPPYNGIDYVVCMGSAFGTA
                     HGAQKVLNMKDDNEKRLVGVLGDSTFFHTGINGLLDVVYNRGNSISVILDNRITGMTG
                     HQENPGSGYTLQGAKTKEVDIEGLVKACGIEHVRVINPNNLKEVNEALDWALAIEDEP
                     SVIITRWPCVLKKFSKEDIEEFNNPFKTKCKVDHDKCIGCKLCLKTGCPALSFDKENK
                     LSNIDRNQCVGCGVCAQVCPKQAIVKEEK"
     gene            6280..6855
                     /locus_tag="KR250_000759"
     CDS             6280..6855
                     /locus_tag="KR250_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HBH0066724.1"
                     /translation="MTKSILLVGVGGQGTILASKLLTMGLMEAGYDVKMSEIHGMSQR
                     GGSVSSQVRYGDCVHSPVIEIGGADILVSFEKMEALRWFNYLKPEGKALVNNHRIDSM
                     TVLIGGAEYQESDIDAELSRLNAKVINAADKAEELGNSKIMNVILLGCLVKSMQLESI
                     DWEKIISENVKPKFVELNIKAFHEGMEMVSK"
     gene            6908..7978
                     /gene="buk"
                     /locus_tag="KR250_000760"
     CDS             6908..7978
                     /gene="buk"
                     /locus_tag="KR250_000760"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HBH0066725.1"
                     /translation="MTYRILAINPGSTSTKIAVYDGEEQILVKTIDHPAEEIAKYNTI
                     QDQFEMRKEAVLNILKENSIDLKSLSAIVGRGGVLPPVKSGAYLVNEEMIDVLRHRPV
                     LEHASNLGAVVAHAISEPLGINSYIYDSVAVDELIDVARISGLCGMDRSSAGHALNTR
                     AMALKYAKDKGKDYKSLNLIVAHIGGGVSIYLHEKGRMVDMLSDDEGPFSPERSGRVP
                     ATKLVAACYSGQYSEREMTKKIRGKGGIVSYLNTVDAREVEKMIAEGNEEAKIIYEAM
                     AYQLAKGIGELATVVDGKVDAIIITGGIAYSEMFTSMVKKKVEFIAPVEIMAGENELE
                     SLAFGTLRVLNGEEEARIYSEN"
     gene            8196..8897
                     /locus_tag="KR250_000761"
     CDS             8196..8897
                     /locus_tag="KR250_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0066726.1"
                     /translation="MGKISLKSMIRLFSGFFIFAISSVLMINAHVGLMPWDVLHQGLS
                     IKLGITIGQASIMVGVVIVILDAVFGENIGWGTLLNMTFIGIFIDLVIFSGVIPHASN
                     TYIGVFMVVIGIILAAIASFLYLGVCLGSGPRDGLMIALQKKTNKSVRLVRTILEILA
                     LVVGWLLGGSVGIGTLVSALGLGYVLQIVFRIFKFDTKLLKHRFIIDDIREWKEKKSN
                     EHKCKSSIVIKNEQN"
     gene            8957..9463
                     /locus_tag="KR250_000762"
     CDS             8957..9463
                     /locus_tag="KR250_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HBH0066727.1"
                     /translation="MELWDLYNADGIKTGNVIKRGNSIEEGYYHLAVEVWILNSNSQI
                     LIQKRSKSKKTLPNMWGMTTGCIVSGEDSLEGAIREAKEEIGIDITKDEMKVFRSMIH
                     EDTLWDVYLVKKEYDISKAILQEEEVSDIKWVSTDEIRQLLKEGLFFEYPEIYELLYE
                     IDNNKLNI"
     gene            9638..10660
                     /locus_tag="KR250_000763"
     CDS             9638..10660
                     /locus_tag="KR250_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBH0066728.1"
                     /translation="MEDNKGLARDSSKYKIVSISVVVIILITFWYMLNMVLLTFIMTF
                     IFYNLLVATRKRIKKFSSLNIPDSLIIIVLYALFAILLVLISYAVVPIIIVQLTELSR
                     VFSDFDVNQFAQSLGPKLYPIVSKLDFNKYISQAGLLIASTATKVGSFGVNILLAFLL
                     SLLLLLEKNEIKNFGDKLSDSKISFIYNSLVFFGKSFVKNFGEVMKVQVMIAFINSVV
                     SMIFLGFMGFPQIWALGFMIFVLGLIPVAGVIVSLIPLTVIAFNTGGITKVFGVLLMI
                     CIVHAVETYILNPKLMSNRTKLPVCFVFIILLVGEHYLGVWGLLIGVPIFMFLMDILG
                     VKFTSR"
     gene            complement(10807..11115)
                     /locus_tag="KR250_000764"
     CDS             complement(10807..11115)
                     /locus_tag="KR250_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HBH0066729.1"
                     /translation="MKNNNLNCAATNCAYNTSGYCYAGSIKVDGMQATTTENTYCASF
                     EDKYTSGITSRSNETNQVDTDNIHCEAVKCKYNKNELCKAEKVHINERNASCETFEMK
                     "
     gene            complement(11175..11603)
                     /locus_tag="KR250_000765"
     CDS             complement(11175..11603)
                     /locus_tag="KR250_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0066730.1"
                     /translation="MNNKLHELEKNLPEISCSFCTHLSFKGPSLDYRYDIRCVISDTK
                     PDFRGCCEYFEPEYTELNTGDLDNLYINFLETCLKVNYNDYINSMYWKLFKERTLLEN
                     NFKCSICGSTENVDVYHVNKNLGRETSKDVVVKCDKCNCK"
     gene            complement(11826..13283)
                     /locus_tag="KR250_000766"
     CDS             complement(11826..13283)
                     /locus_tag="KR250_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HBH0066731.1"
                     /translation="MTTFLIGLAILLIGGALYGAYCEKVFGPDDRKTPALAQSDGVDY
                     VPMKKWKNSLIELLNIAGTGPILGPIQGILFGPIAFILIPIGCVFGGALHDYMSGMIS
                     IREKGAQMPSLISRFLGNKVFQVYNIFLCLLMLLVGAVFIYTPGDLVVTQILNMKSTI
                     NNPVVWIVYGLIFLYYLCATLFPIDKIIGKVYPIFGAILLLSAAGVGIGIFTQGYDLA
                     NLSLANWKGIHPDGIPLIPTFFVTVACGIVSGFHSTQATLIARSVSNEKEGKTTFYNM
                     MILEGLIAMIWAAAAMGIYNKGIPKELVGSPDVIGLVARDLLGSIGGIIAIIGVIVLP
                     ITSGDTALRSLRLMLADYFHYDQKEKKHRVILSICIFIPVIAILIFAKLSASGFNILW
                     RYFSWSNQTIAIFAFAMITVYLIIKEKNYIISLIPGMFYSFVIFSYIFNAQIGFNLNI
                     NISYVLAAIFTVLYAILTVRSGRKLKSKADTKLAD"
     gene            13701..14615
                     /gene="nadA"
                     /locus_tag="KR250_000767"
     CDS             13701..14615
                     /gene="nadA"
                     /locus_tag="KR250_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HBH0066732.1"
                     /translation="MDKDLTYQIKELKKEKNAIILAHFYQPPEIQELADAVGDSYYLS
                     EIARDCKEEVVVFCGVRFMGESAKILSPEKTVLMPVSNAGCAMADMVDEEGVIKLKQQ
                     YPNALVVCYINSTAKVKAHCDVSVTSSSAIKILENIDNKEIIFLPDKNLGGYIAEQFP
                     DKNFIFWDGYCKYHNNIRAEEIIELKDKYKNAEVLVHPECKKEIRDLGDYVGSTSGII
                     KYATNSKNKDFIIATEEGILHELKKNNPNKNFYIPGGKILCTDMKKTTLENLYSTLKN
                     MENEVIVEDEIMEKALNSLLNMHKLAEG"
     gene            14628..15929
                     /locus_tag="KR250_000768"
     CDS             14628..15929
                     /locus_tag="KR250_000768"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HBH0066733.1"
                     /translation="MNLEQDVLIVGSGVSGLYCALNLDKSLNVLVVSKSTIENNNTYL
                     AQGGISTARNIDDIESFVEDTMKAGQYKNRVEAVQVLADESIENVGQIVEYGMPLDKE
                     NGEIDYTREGAHSVNRIVHSKDNTGEVVFKTLLKEAKTRENITLIEDAYLLDILKDGN
                     KCIGARIFKSKKEIHVFSKIVVLATGGIGGLFKNSTNQRHLTGDGIAIALRNNIKIEN
                     LEYIQIHPTAFYEENNEGRRMLISESLRGEGAKLLNKNKERFVDELLPRDVVSKAIFE
                     QMEKDKLPYVYLDATHLDSEYLINRFSFIYNECLARGTDITKECIKVSPAQHYFMGGI
                     HVDLDSKTSMENLYAVGEISCTGVHGANRLASNSLLEGLVFSKRAAKNINSVIDNVKV
                     KFIDVPDMDIDIEQVKKENKILVIKEIERTSEDFGDELFDY"
     gene            15910..16746
                     /gene="nadC"
                     /locus_tag="KR250_000769"
     CDS             15910..16746
                     /gene="nadC"
                     /locus_tag="KR250_000769"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HBH0066734.1"
                     /translation="MNYLIIDRMIQDALIEDVPSEDITTNSIVDENSKSTVDLICKQD
                     GIIAGLGVFKRVFEILGDVDVKLYKNDGDRVKNREKIAVLTGSTRNLLVGERVALNYL
                     QRMSGIATITSRYVEKLEGTNTKLLDSRKTIPNLRILDKYSVKVGGGCNHRFNLSDGI
                     LLKDNHIGAAGSVKKAVELARKNTSFVRKIEVEVETLDMVKEAIEANADIIMLDNMSL
                     EMAKQAVDIINGRAIVEFSGNVNLDTIEDIGKIGVDVVSVGALTHSVKALDISMKNLR
                     NI"
     gene            17002..17334
                     /locus_tag="KR250_000770"
     CDS             17002..17334
                     /locus_tag="KR250_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HBH0066735.1"
                     /translation="MKVAYIFSSTNTHKILDKMIIPQLEQGTHGVDVLGMFFFMDNTL
                     FLSKGNPVGERLSKLHEKTDMIIMACDQCAIERGIENNLVDGATIGCFPNLYACLGSA
                     GVDQVITL"
     gene            17496..17681
                     /locus_tag="KR250_000771"
     CDS             17496..17681
                     /locus_tag="KR250_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0066736.1"
                     /translation="MSEKYADKLEIKLYQAGKDFSYIKKYGIITKGTLIINQKKKYDR
                     LNKDTIERAIVEAINNN"
     gene            17708..18793
                     /locus_tag="KR250_000772"
     CDS             17708..18793
                     /locus_tag="KR250_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HBH0066737.1"
                     /translation="MNILEIVLNGVKDTIVASIVTLNGASGWLMISFILAGVLHNVLT
                     PRRFQKQLGNKKFSSLIKSTISGMFLPICSCGVIPLGISMYYSGAYLGPVLAFMTSTP
                     IINPIAVVLCLGLLGPKVTIIYLIAGFLVPFLVGFLGNCFGKEELSLHNDSEEEVIEL
                     EEEKRSFMENMIDGLKWSFGELALTISKYVVMGMVFAGFITTIFPNSIIQKYLGNPGL
                     LSLGSIAILACVMYVCAVGHIPFIAALVASGASPGIAITFLIAGAATNLPELISMFKL
                     IGKRTVIIYSVTLTLSSIIIGYITNLLLVNNNSVNLDATNSSIQLANKLMLNIPDSFE
                     YVCTILIILFAFKSIIPKMKEVIGNHA"
     gene            18786..19244
                     /locus_tag="KR250_000773"
     CDS             18786..19244
                     /locus_tag="KR250_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0066738.1"
                     /translation="MLKKIVIISCIVVVLIILSKIVDDNIKEDASIPNVNKETLEYFR
                     KNYKEDIITCAEEDLNNDGKKDLVVIYKKSNNSNEMVVVVSDKNSHYITKPIPAPIEN
                     QTITFKNIDDKAPIEVIVSGSKNGNVGYAIYRVEGKKFVDLFGEDMDKCC"
     gene            19279..20370
                     /locus_tag="KR250_000774"
     CDS             19279..20370
                     /locus_tag="KR250_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBH0066739.1"
                     /translation="MKLSKKIQALILFGVMGTSILTGCSSKPKEKEEAKTGKDDYTIT
                     VGYYNCDHMTAGPVAESAGIYKDLGLNVKTVGNGKVPEAMAAGKMDAGYIGTKGLVGA
                     IPKGSPITIAANNHTGGSEYLIVSKDIKEPKDLIGKKIATDMSDFLWTSDYGPETGLP
                     TDPSKYEVVNMDSDKDKYLALKTGKIQAFTSCDPWGSVAENDGAGKIIASTQYKEKAN
                     GKEYNCCSFSLNKNFIKEHPDLAKKLVLAHTKAIEYIYTNPAEAAKIFAKYYNVEEEV
                     ALRTIYKKTVGEGRTLTWKVTGEEYKNNLQMYKDLKALDDIPKYEDTIDTSLLDSCGA
                     DDFDKFIKEKVNPEFPEGMSYEDWKAKVL"
     gene            20375..20599
                     /locus_tag="KR250_000775"
     CDS             20375..20599
                     /locus_tag="KR250_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0066740.1"
                     /translation="MENMKNIIILFIIILGSIFIIFFNFEDKEEIKGKLRIGVSDDTS
                     GFVINYMVNRDYFENIKVSDIMDIFTINDC"
     gene            20609..21172
                     /locus_tag="KR250_000776"
     CDS             20609..21172
                     /locus_tag="KR250_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0066741.1"
                     /translation="MQWALSSEEIDIAIICKDAAKQYVNVNSGFEIVGTVVQNSDIFL
                     LSDNNPEKIGVIQNRDYQSELVKKYYKNVEVAPLLGTALAYGLESNLVDGVVIDCIKS
                     IGLEGKRKSTTKLGDYDTYVLVVSKEFKKSKAYSDFITLYNKSVDALKNKATFKKALK
                     EYRNIRNIDMEEISNWKLKFLKIKAID"
     gene            21133..21762
                     /locus_tag="KR250_000777"
     CDS             21133..21762
                     /locus_tag="KR250_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0066742.1"
                     /translation="MEIKISKDKSDRLTKKKTDGSMDILYKIILLVIIFLVWHFAAKD
                     IGSSLLLPMPVDVIKGFFFCVTDAETVTNLFITLQRVLKGFMYALLFGLPIGFIMGFS
                     KTFERVLSPVVDSVRQVPIMAWVPLTIVWFGIGDGPTIFLIAFSGVFPIILNTIQGVR
                     AISKDYYNAARSMGASPIVIFTNVIVPASLPDILTGSRIAISTGWMSVI"
     gene            21802..21939
                     /locus_tag="KR250_000778"
     CDS             21802..21939
                     /locus_tag="KR250_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBH0066743.1"
                     /translation="MVQAQTLMHTDVLIGLMIFAALIGFIIDRVLKFINKILCRWRFA
                     D"
     gene            21939..22691
                     /locus_tag="KR250_000779"
     CDS             21939..22691
                     /locus_tag="KR250_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0066744.1"
                     /translation="MKLSVRDINKTFVNNRVHTKVLEDISMDIDDGQFVCLLGPSGCG
                     KTTLLTIIGGFQKSERGDVFINDKRVKKPGIDRAFIFQNYALFPWKTIRGNVLFPMKQ
                     QKIPKEKREEMLEELLVMSDLKGKEKLFPHQLSGGMKQRVAMIRALACNPEVLLMDEP
                     LGAVDFQMRQNLQEELERIWIKKKITALMVTHDVDEAVYMSDRVIVMSRDKGRIIDDI
                     NIDIPRPRIRGSQKYEEYKNKLTDTLSKCYEV"
     gene            22704..23189
                     /locus_tag="KR250_000780"
     CDS             22704..23189
                     /locus_tag="KR250_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0066745.1"
                     /translation="MSQIEKYNSLDNTQQKIRISIMDMIIDKGSSVTLQEVTEYISKK
                     LNIEKEYIERTLQYFIYKNIMVVDGNSINFIYPVSALPTNHKVTLRDNRSFSAMCAID
                     AIGTSCTFNQDIKINSICSATGKEIEVIIKNEKIEYVNNPNLRVLHINLDKHLNWAAS
                     C"
     gene            23196..23345
                     /locus_tag="KR250_000781"
     CDS             23196..23345
                     /locus_tag="KR250_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0066746.1"
                     /translation="MNFFWTKSDFDAWTNEAGLSNDEDIYCLDINEAIVESYKIFKLK
                     QKVLS"
     gene            complement(23482..24303)
                     /gene="yidA"
                     /locus_tag="KR250_000782"
     CDS             complement(23482..24303)
                     /gene="yidA"
                     /locus_tag="KR250_000782"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HBH0066747.1"
                     /translation="MYKLIALDIDGTILNTQKRITPEVFESIQEAKRAGAKVVITTGR
                     PLPGVKELLNQLNLTDEGDYVICFNGAIIQEVKSEKIIHDVEMSLDDFDFIYNNVCKK
                     YKTKIHINTMTNLITPNETPGKYTLHEAKLNNIEVKYIQKDKIDESIKICKIMIVDEP
                     ERLEEIIQQLPKNLFNKYTIVRSAPFYLEFLGKTTNKGTALKTLCTNLNIPIENAIAV
                     GDEENDQHMIKYAGLGVAMGNARNSIKEIADYVTDTNNENGVAKVINKYILNKAI"
     gene            24796..26490
                     /locus_tag="KR250_000783"
     CDS             24796..26490
                     /locus_tag="KR250_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HBH0066748.1"
                     /translation="MKFSEFKYERPNYNSMKKEFLSCVEDINNSRNYKEQQKNIHKIN
                     LLRNKIETLSNIASIRYSTDTFNKFYKEEKNYWDEYMPLYEELNSYFYNAIVNSKFKY
                     DLIKEFGEQFFTIVEYSLKSFSKEIISELQEENKLCSEYTRLLASAEIMFDGKIRNLS
                     GMGKFMYSKNRKTRELANKAYYNFFEENETKFDDIFDKLVKLRDKMSKKLGFEDFVEL
                     GYVRMMRSDYREYMIKNVRKQVLKYVVPMANELYEKQAKRIGLEYLSYIDEGVEFLTG
                     NASLKGDSRYIIKNGKRMYSELSKETNEFFDFMLENELMDLETKKGKGAGGYCTYIPD
                     YKSPFIFSNFNQTADDIDVLTHEAGHAFQLYMSRWIDMPEINFPTLDSCEIHSMSMEF
                     ITWPWMDLFFKEDTDKYKFTHLSSSIKFIPYGVIVDEFQHYIYKNPNVDKSKRKEIWR
                     FLEKKYLPHRKYGDNSFLERGCWWFKQGHIFKNPFYYIDYVLAQICALQFWKKMIQDR
                     DAGWKDYINICKVGGTKSFLDIVSMGNLYSPFDDGCIESIIGDVKSWFDEINDSKL"
     regulatory      26822..26932
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            27036..27407
                     /locus_tag="KR250_000784"
     CDS             27036..27407
                     /locus_tag="KR250_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrdX family protein"
                     /protein_id="HBH0066749.1"
                     /translation="MIIITNNPKVKEEVQGREVLFKDTTYIGILEASRDLIHEGYELL
                     SHPLYGSVKPNETPYRTVILKKGNRLDINSLTLIEEAIITASKFQNNKKTPKWTESVQ
                     DDFRVIDYDIFYNTIQRMQYE"
     gene            27504..28451
                     /gene="trxB"
                     /locus_tag="KR250_000785"
     CDS             27504..28451
                     /gene="trxB"
                     /locus_tag="KR250_000785"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HBH0066750.1"
                     /translation="MENVYDLVIIGSGPAGLAAGLYGARAKLKTLILEKDKTGGQIVI
                     THEIANYPGSVPNATGPSLIARMVEQCKEFGAEMLRDNIVDTELDGDIKVLKGEKAEY
                     RAKAVIIGTGATPRKIGCPGEKELTGKGVSYCATCDADFFEDFEVFVVGGGDSALEEA
                     MYLTKFARKVTIVHRRQGFRCAKSVEEKAKANPKIEFLLDTVIEEIKGDGILESVVFK
                     NKVTGETHEYFADEEDGTMGVFVFVGLDAQTDLFKGKVDMDEKGYIITDEDMRTNIPG
                     VFAAGDCRSKTLRQVVTATNDGAIASIVAEKYIDEKFGN"
     gene            28555..28872
                     /locus_tag="KR250_000786"
     CDS             28555..28872
                     /locus_tag="KR250_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductase thioredoxin"
                     /protein_id="HBH0066751.1"
                     /translation="MLDLDKATFEEEVLNAEGFVFVDFWSEGCEPCKALMPDVHKLAE
                     TYGDKIKFCKMDTTKARRLAIKQKVLGLPTMAIYKDGEKVDEVTKDDATVPNIENMIK
                     KYL"
     gene            29141..30427
                     /locus_tag="KR250_000787"
     CDS             29141..30427
                     /locus_tag="KR250_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006439082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HBH0066752.1"
                     /translation="MRLELGKIFIKDIQFGDVTEVKDGVLFINKQEMLQEIGGDEHIK
                     SIDIELARPGESVRITPVKDVIEPRVKVEGNGGIFPGIMSKVDTVGEGKTHALKGVAV
                     VTTGKIVGFQEGIIDMTGEGAKYTPFSKLNNVVVIAEPIDGLKQYAHEKAVRMIGFKA
                     AMYLGEVARNLTPDEVSVYETKPLLESIKEYPELPKVGYVYMLQTQGLLHDTYVYGVD
                     AKQIVPTLLYPTELMDGAIVSGNCVSACDKNPSYVHINNGVVEDLYARHGKDINFVGV
                     IITNENVYLADKERSSNWTSKLCKYLGLDAVIVSQEGFGNPDTDLIMNCKKIEMQGVK
                     TVIVTDEYAGRDGGSQSLADADVRADAVVTGGNANQVVVLPKLDKVIGHLEVVDVIAG
                     GSDGSLRADGTIEVEIQAITGATNETGFGHLTAKGY"
     gene            30539..31012
                     /locus_tag="KR250_000788"
     CDS             30539..31012
                     /locus_tag="KR250_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:30671..30673,aa:Sec)
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     selenoprotein A"
                     /protein_id="HBH0066753.1"
                     /translation="MSLLSNKKVLIIGDRDGIPGPAIEECVKTVEGAEVVFSSTECFV
                     UTAAGAMDLENQNRVKDAADKFGAENVVILLGAAEAEAAGLAAETVTAGDPTFAGPLA
                     GVALGLSVYHVVEEPIKSLFDESVYEDQISMMEMVLEVEEIEEEMSGIREEFCKF"
     gene            31044..32354
                     /gene="grdB"
                     /locus_tag="KR250_000789"
     CDS             31044..32354
                     /gene="grdB"
                     /locus_tag="KR250_000789"
                     /EC_number="1.21.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:32091..32093,aa:Sec)
                     /transl_table=11
                     /product="glycine reductase complex selenoprotein B"
                     /protein_id="HBH0066754.1"
                     /translation="MGKLKAVHYINQFFAGIGGEEKADTKPHVAETLPPISLQLDKLL
                     GEDIEIVGTVVCGDSYFNENIDSASEEVLSMVKGFEPQLFIAGPAFNAGRYGVAAGTI
                     TKVVKDALNIPALTGMYVENPGADMFKKDVYVVETSDSAAGMRKALPKIAKLAVKLAN
                     GEEIGTPKDEGYIARGIRVNYFHEDRGSKRAVDMLVKKIKGEPFETEYPMPNFDRVDP
                     SKAVKDLSKCKIALVTSGGIVPKGNPDRIESSSASKYGTYSIAGVMDLTEETYETAHG
                     GYDPVYANLDADRVLPVDVLRDLEKEGVIGKLHETFYTTVGNGTSVANSKKYASEIGA
                     ALVADGVDAVILTSTUGTCTRCGATMVKEIEKTGLPVVHMCTVVPISLTVGANRIVPT
                     IAIPHPLGNPALDPTEEKALRRGLVEKALNALTTEVDGQTVFEK"
     gene            32610..34142
                     /locus_tag="KR250_000790"
     CDS             32610..34142
                     /locus_tag="KR250_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014520415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III family protein"
                     /protein_id="HBH0066755.1"
                     /translation="MTYPVLKGAGYVLIHTPDMIVQNGSTCTVERATNPDSEFLKEVS
                     NHIRSYEDVVNYMPNQVYIGNRRPEELRDLPMPWCEQKIEGTRNGKFGEIMPQDEFIA
                     LMQISDAFDLVKLSQEFIDEVKPKIENNYPEIAPFVGKLKGDDIEEGKELVATHIAEG
                     LYHDGKFVGYVKRAHDVDVNLNAHTMFENLVVKASGVLSAIQMLRHSKIDPAEIDYVI
                     ECSEEACGDINQRGGGNFAKSIAEIAGLQNATGSDTRGFCAAPTHALIQAAALVKAGI
                     HKNVMVVAGGASAKLGMNAKDHVKKGLPVLEDVVGGFAVLVSENDGVNPVIRTDLTGK
                     HTVGTGSSPQAVMTALITSGLDRANLKITDVDVYSVEMQNPDITKPAGAGDVPEANYK
                     MIGALAVKRGDLEKKELKDFVSNKGLPGWAPTQGHIPSGAPYIGFLIDDLTTGNRNRA
                     MIVGKGSLFLGRMTNLFDGVSFIAERNTGVTEETSGISKDEIKKIIAESMKKLALDML
                     EE"
     gene            34153..35298
                     /locus_tag="KR250_000791"
     CDS             34153..35298
                     /locus_tag="KR250_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine reductase"
                     /protein_id="HBH0066756.1"
                     /translation="MSKKVIADVFLEVANAIESGEFGKKVKIGVTTLGSEHGVENMVN
                     GAQLAKSNLFDIVLIGPKVETDLEVVEVNDEKEMHAKMEELLDSGYIDACVTMHYNFP
                     IGVSTVGRVITPAKGKEMILATTTGTSATNRIEAMVRNAIYGIATAKSMGNKCPKVGI
                     LNVDGARQVEKCLKELKDNGYDMEFADSIRADGGCVMRGNDLLVGAPDVMVTDTLSGN
                     IFMKVFSSYTTGGDYEAQGFGYGPGVGEDYDRKVLIVSRASGSPVVANALKYAYDVVK
                     GDISNVARNEFAKVKKAKFDDIISSLTKKEVKAEKVEVKMPDKEIVTRQIAGVDIMDL
                     EDAVSELWKNGIYAESGMGCTGPIVLVNEAKGDLAVETLVKAGYTAK"
     gene            35608..36687
                     /locus_tag="KR250_000792"
     CDS             35608..36687
                     /locus_tag="KR250_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBH0066757.1"
                     /translation="MSRVKNVVELLETKGVDALYLTKKTNVNYISGFPDEEAYAVICK
                     DGNFLVTDSRYMELAEKVCKDFEIINWHNFDRSVAKAVKSVCDKVGIKKLGFERTNIV
                     FDKYEELKNLIEKDNGELIPTENIVETLRYVKDKDEIKNTRKACEIADKALEELIPHI
                     KAGVSEIELATRLEYFMKMNGAQNIGFETILISGAKTSLLHGKPSDKIIEKGDFVLID
                     YGAMYNGYISDTTRTFIVGGASEKQLEIYNLVKEAQNVGVENMKAGVHATIPDAEIRK
                     VVKKYEDYYYQGIGHGVGRDVHEEPFIGNYGDKIIEEGCIITMEPGIYFPGWGGVRIE
                     DTVLITKNGPERLTKFPKDLMILDK"
     gene            36800..37399
                     /locus_tag="KR250_000793"
     CDS             36800..37399
                     /locus_tag="KR250_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0066758.1"
                     /translation="MYKKHKFIKNIKKAWYKIKLIDRCLIIVMAILMFQSIYNLFVNE
                     VNSQDTTTIDVVVRTTSAAIFGYFLSANFIKRPLRKYTNTDVSSNLFIDNNLSPKGQE
                     SNQNNIMNVKNTIGFTSEADNYEKKIPINNNEGFEEGETSELQIIIATVICIVALTVL
                     FVVRNFTTTTSASLGTISQMRDFVSGCVGFLLGCPSKPD"
     gene            complement(37586..38458)
                     /locus_tag="KR250_000794"
     CDS             complement(37586..38458)
                     /locus_tag="KR250_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBH0066759.1"
                     /translation="MNINYLYYFQTVCKYKNMTKAAESIHISQPSITLAIKELEKELG
                     FELFYRIGNKIELTPEGKIFLDKSKHFIKQFEDFQCDALDLGKKRKASLKIGIPTVLG
                     TFLLSKILPRFNVIYPDIELKIFEVPTFVGAKMIEESTLDFCIGIIDSDIYDDIDSKT
                     IYKTELYLVTNPKNELAKHPIISNYMLKNVPFVILSEGSYHYKIITKRLEKAKPNIIL
                     HSNQLSTIRYLLENDLASTILYKEIFQNTENLCSIPLERAITANIGVLWRRNQYISHS
                     MKLFIEYMASIHIN"
     gene            38875..40278
                     /locus_tag="KR250_000795"
     CDS             38875..40278
                     /locus_tag="KR250_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0066760.1"
                     /translation="MKGSDLVKFELMDFLMNPFVLMFAAVITGILFGKIKFGKFNFGV
                     SGALFTGLFIGWLAYSLGNLIIAKGETAAGYKAATVMMGNGIISSDFFDFFLIIFVAA
                     VGLLAAKDMKAVLKKYGARFVILGVLITFIGGSMTYAMTLLSSDKGSSAYEVSGVYTG
                     ALTSSPGLAAALETAGKHAEDVSKEFEKASIKDKKEILKVVDPEGKLDVNTTTSLTQE
                     QIDKYIAYAEAGVGIGHAVAYPFGVLIVILGVNFLPKLFRMDLKEERRKYEKEMKEAR
                     DSVSGKTIPEVPFNIITFFLTCLAGYLVGGIHVFMGPLGYFTLGATGGSLIVSLVLGY
                     IGKIGVVNFRMEEKVLNILKQIGLVFFLAIVGLRYGGKVVDSIMTSGMHLALVAIAVG
                     VTAMMIGFLVGKYVFKLNWILLSGAVCGGMTSTPGLGAAVDALDSDDPAAGYGATYPF
                     ALLTKVILVIVLHKLPM"
     gene            40305..41849
                     /locus_tag="KR250_000796"
     CDS             40305..41849
                     /locus_tag="KR250_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase family protein"
                     /protein_id="HBH0066761.1"
                     /translation="MNNEGAFEELSGRLRNKELCDLIMNADEAAKIIEDGMVIGVSGF
                     TPSGYPKAVPLAVSERAKSGEKIKLTVYSGASLGPEVDGAWSEAGIIERRLPYQTNSI
                     LRNNINKGVVDYIDMHLSHSTQFLNYGTIPKVDVAIVEALAITEEGNIIPTSGIGNSP
                     SFIKSADKVIVEINLAKPMEMEGMADIYITENPPNRKPIEINHPKDRIGTTYIPCGLD
                     KIAGVVITNMQDKTRPLGVVDEASKKISNNIIAFLREEVKSGRLSKNLLPLQSGVGSV
                     ANAVLYGLCESEFENLTCYTEVVQDSMLDLIRMGKVTMASTTSVSPSPEGLIKFEKDI
                     DFFKDKIILRPQEISNNPEIARRIGVIAMNTAIEVDIYGNVNSTHIMGSKMMNGIGGS
                     GDFARNGAITIFSTESIAKNGDISSIVPMVSHVDHTEHDVMVIVTEQGYADLRGLAPR
                     ERAIKIIENCAHPDYKEQLRDYLNRACKSGAKQTPHILDEALSWHSKFMSTGTMKKAE
                     TFKSAL"
     gene            41880..43271
                     /locus_tag="KR250_000797"
     CDS             41880..43271
                     /locus_tag="KR250_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HBH0066762.1"
                     /translation="MEKAVENFEDLSKEYINGYIERARKAQREFECYTQEQVDKIVKI
                     VGKVVYYNAEYLAKLAVEETGMGVYEDKVAKNKSKAKVIYNNLKDKKSVGIIDIDRET
                     GITKVAKPVGVVAAITPCTNPIVTPMSNAMFALKGRNAIIITPHHKAIGCSTKTVEMI
                     NEELEKIGAPENLIQILDQQSRENTRNLISSADVVIATGGMGMVKAAYSSGKPALGVG
                     AGNVQCIIDRDVDIKEAVPKIIAGRIFDNGIICSGEQSVIVAEEMFDKIMDEFKNNKG
                     FIVRDKVQKEAFRNAMFVNKSMNKDAVGQSVHTIAKIAGVEIPEDTKIIVIEADGPGE
                     EDIIAKEKMCPVISAYKYKSFEEGVAIAKANLNVEGKGHSVSIHSNTVKNIEYAGENI
                     EVSRFVINQCCATSAGGSFFNGLAPTNTLGCGSWGNNSISENLDYKHLINISRIAYYM
                     PENEVPTDEELWG"
     gene            43464..44960
                     /gene="abfD"
                     /locus_tag="KR250_000798"
     CDS             43464..44960
                     /gene="abfD"
                     /locus_tag="KR250_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybutyryl-CoA
                     dehydratase/vinylacetyl-CoA-Delta-isomerase"
                     /protein_id="HBH0066763.1"
                     /translation="MALMTGAQYIESLRKLNTKVYMFGEEVKNWVDHPMIRPSINCVA
                     ATYDLAHDPEYADLMTVTSNITGEKINRFGHLHQSVDDLIKKVKMQRLCGQKTASCFQ
                     RCVGMDAFNAVYSTTFECDKAHGTNYHDNFVKYLTYIQENDLVVDGAMTDPKGDRSLS
                     PSAQPDPDMFLHIVERREDGIIVRGAKAHQTGSINSHEHLIMPTISMTEADKDYAVSF
                     AVPSDAEGVFMIYGRQSCDTRKLEEGADVDLGNKEFGGQEALVVFDNVFVPNDRIFLC
                     GEWDFSGMLVERFAGYHRQSYGGCKVGVGDVIIGAAALAADYNGANKASHIKDKLIEM
                     THLNESLYCCGIACSSEGHKTEAGNYQIDLLLANVCKQNVTRFPYEIVRLAEDIAGGL
                     MVTMPSEKDFKSDLKVGTSGMTIGEVCNKYFKASSVASTEERMRILRFLENICLGSSA
                     VGYRTESMHGAGSPQAQRIMISRQGNINQKKELAKKIAGIKKEEALNL"
     gene            45095..45370
                     /locus_tag="KR250_000799"
     CDS             45095..45370
                     /locus_tag="KR250_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0066764.1"
                     /translation="MTCGVRFCGGCNPRYERGKALTIIKEHFNGRVDFSIAQENVEYD
                     LLLIIGGCATLCASYQQYKVKNECVKMWDFSHIERVINVIESAICET"
     gene            45394..46701
                     /locus_tag="KR250_000800"
     CDS             45394..46701
                     /locus_tag="KR250_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase family protein"
                     /protein_id="HBH0066765.1"
                     /translation="MSWQELYQSKLCSATEAVKQIKNGDTVVFAHCVGEPPALVEAMI
                     ENAEQYKDVEIKHMVSLGSGGYTAKGMEAHFRVNPMFVSGNVRKAIENGDGDFTPAFF
                     HEVPKLLREKRLKCDVVLAQVTPPDEHGYCSLGTSVDYTYEAIKTARTVIVQVNDQFP
                     RTYGEVVHVSEFDYIVEKSQPLFELQPAKIGEVEEAIGKNCASLIEDGSTLQLGIGGI
                     PDAVMLFLTDKKDLGIHSEMISDGTLALYEKGVINGKYKNFDKEKMTVTFLMGTKKLY
                     DFANNNPAVEVKPVDYVNHPAIIMKQHKMVSINSAIQVDLMGQVVAEAMGLRQFSGVG
                     GQVDFIRGVSMGEDGKAIIAMPSITTKKDGTVISKIVSIVDEGAPITTSRNDVDYIVT
                     EYGIAELKGKSLRERARNLINIAHPSVRESLAVEFEKRFKEKY"
     gene            46721..47839
                     /locus_tag="KR250_000801"
     CDS             46721..47839
                     /locus_tag="KR250_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybutyrate dehydrogenase"
                     /protein_id="HBH0066766.1"
                     /translation="MEALRVVPKIFYFDTFKEFNEEFKIGKNDLVITNEFIYEPYMKP
                     LGIDTNLIFQEKFGTGEPSDEMIDSMTKEMKKYNFDRIIAFGGGTIVDICKILALDVP
                     EKSIDLFEGEVSPKKVKELVVVPTTCGTGSEVTNVAIAELKSKHTKKGLAVEETYADY
                     AVLIPETVKGLPYKFFVTSSVDALIHAIESYLSPKASPFTEMYSLQAIKMIMDGYKKI
                     VDKGEEERFNHLRDFVLASNYAGIAFGNAGCAAVHALSYSIGGAFHVAHGEANYQFFT
                     EVFKMYSRKKPNGKIIKCTKILADALECDPNCDVYGELEKFLNKLIAKKALREYGMVE
                     SQIDEFTDSTIANQQRLLANNYVELSREEIREIFANLY"
     gene            48126..49046
                     /locus_tag="KR250_000802"
     CDS             48126..49046
                     /locus_tag="KR250_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HBH0066767.1"
                     /translation="MYKSENFSKKSVLINSFLSKFLKIIGWGLVSLFTLSALVLSTIS
                     IIIGWAVILPIQVCAVFLLIGGVCIFKGKRLGDQISRYKKYCTIINNRNIIPVELIAE
                     NTSKSLNFIIKDVQKMIDKNYFINTYIDKRNNQIVLTNEDFVPPRYEDVAYEVKEEEK
                     TDESNEVDTIINKGMNYLTQIKEANKNIKSESMGDKIVQVEDVTSKIFDVVKHDPSKL
                     TQIQKFMDYYLPTTLKLLNSYHTLEEQGIDRENITTTMESIENTMNTIVVAFENQLDY
                     LFEDEAIDISTDITVLENMLVQEGLTSGKF"
     gene            49161..50318
                     /locus_tag="KR250_000803"
     CDS             49161..50318
                     /locus_tag="KR250_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HBH0066768.1"
                     /translation="MNNLDDIPVMPTLTLDPFGEESNTNDIDNSDLLMKKDEKDPEEE
                     KLSESERKMVKEFAEKIDITNTNMILQYGAGAQKKVASFSETALKSVKTRDLGEVGDM
                     LTNLVTDLKAFSADEKEQSGFLGIFKKANNKISNLKTKYDSAEVNVDKVSKELQKHQV
                     KILKDIAMLDKMYELNLAYSKELTMYIIAGKQKLKDMKEYEMPKLREKARLSGSTEDA
                     QSVNDMVSLCDRFEKKIHDLELTRMVSLQMAPQIRLVQNSNNLMAEKIQSTIVNTIPL
                     WKNQIVLALGISHLNQAMKAQREVSNMTNELLLKNAETLKMGTIETAKESERGIVDID
                     TIKKTNQSLISTIDEVVKIQHDGRIKRQEAEVELSKIENELKSKLLEFTVK"
     gene            complement(50399..51295)
                     /locus_tag="KR250_000804"
     CDS             complement(50399..51295)
                     /locus_tag="KR250_000804"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBH0066769.1"
                     /translation="MLKKVKYPIGQVKVSDKFKTIKLLSRKYSISSMCKLISVSRSGY
                     YKWLSYSKKSSDRGIKDRIIKDYIIEIHKKYRGTYGRKRICTYLNKILDTPINHKRVY
                     RLMKELGIKSIIRKKVYRRKFKSYEIYDNILNREFRANQPLEKICMDITYIPIGKKFL
                     YMNVAKDLFNGEIVAYEISTKMDTKLVNNTVNQLINMNLAKDCILHTDQGSQYTSRSY
                     SKRLKDNGIIQSMSRRGNCWDNAPIESFFSHFKSELIYLIDTTDPKEMISLINDYIYF
                     YNNERIQLKNGMSPIEYRTHSE"
     gene            complement(51253..51570)
                     /locus_tag="KR250_000805"
     CDS             complement(51253..51570)
                     /locus_tag="KR250_000805"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBH0066770.1"
                     /translation="MSKKHKKYSKELKLKAVNLYIKEGYSSYKIAEMLNIRSKTQVQN
                     WVKDYKNKGKTAFDDETRGRFKNISLENDNRKFKSVEEELKYLRMENEFLKKLSTLLD
                     KSK"
     gene            complement(51707..53206)
                     /locus_tag="KR250_000806"
     CDS             complement(51707..53206)
                     /locus_tag="KR250_000806"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HBH0066771.1"
                     /translation="MAFYFDTPSHTFSEYLLVPGFSSTDCIPANVSLKTPVTKFKKGE
                     EADIYMNIPLTSAIMQSVSDDKMAIALAKEGGISFIYGSQTIEDEAAMVARVKSHKAG
                     FVVSDSNIKPDNTLKDILDLKEKTGHSTVAVTEDGTSTGKLLGIVASRDYRISRMDLD
                     TKVSEFMTPMSSIVYANKDVTLKEANNIIWDHKLNSLPVLDDNGNLMYMVFRKDYSSH
                     KENPLELLDSSKRYVVGAGINTRDFAERVPALVEAGADVLCIDSSEGFSEWQKITLDF
                     IREKYGDTVKVGAGNVVDREGFLYLAEAGADFVKVGIGGGSICITREQKGIGRGQATS
                     IIEVAQARDEYFEKTGIYIPICSDGGIVYDHHITLALAMGADFIMLGRYFSRFDESPT
                     NKVNINGSYMKEYWGEGSARARNWQRYDLGGDKKLSFEEGVDSYVPYAGSLKDNVTLS
                     LSKVRSTMCNCGALSIPELQKNAKLTLVSSTSIVEGGAHDVMLKDNRNL"
     gene            53802..55226
                     /locus_tag="KR250_000807"
     CDS             53802..55226
                     /locus_tag="KR250_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HBH0066772.1"
                     /translation="MSSSNQSTIKTSIQRVGKFLSGMVMPNIGAFIAWGLITALFIPT
                     GWMPNEKLSTIGDPMIKYLLPLLIAYTGGKAIAGQRGGVIGAAAAMGVIVGADIPMFI
                     GAMIMGPFAGWVIKKFDKFVDGKIPTGFEMLVNNFSIGIIGMLLAILGFYAIGPAIVA
                     GTALIESGVQFIVSKSLLPLVSVFIEPGKVLFLNNAINHGILGPIGIAEAKEAGKSIM
                     FLLESNPGPGLGVLLAYWMFSRGSVKQSAPGAVIIHFFGGIHEIYFPYILMNPVLILA
                     TMAGGAAGILTFSILGAGLVAAPSPGSIFALMALAPKGGLLPVLAGVAVATVVSFLVA
                     APFVKRASANESEEDSTSLEEAKAKMSDMKSASKNSEKNIEKKQLEVNEIKKIVFACD
                     AGMGSSAMGASRFKNRIKNLDLDIEITNSSVDNLPDDTQIVVTHNTLVERVAKNNSSV
                     EIVSINNFLNDPNLDTLFKRLESK"
     gene            55261..57390
                     /locus_tag="KR250_000808"
     CDS             55261..57390
                     /locus_tag="KR250_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBH0066773.1"
                     /translation="MKKKKITSRQKKIILMIVENSKKNIPITISEIAGTLELSSRTVL
                     RDMSGIEKWFDENDFNFVKKPGVGLILEENIENQNFIIELLEEEKIEKEYSKEERNLI
                     ILSKLLVSNEPVKSYYFAKILKVSEGVLNNDFSLASKWLERFDIELVRKPGLGVYLKG
                     QEKSFREAYVNLIYDSFNEKEILDMVRNISENIQTDKAIEILSENRLLNLMDRCIIIK
                     VEATLTKKLSDLDVNLADSAYIGLVVHISLALQRIKNGENITMDKEFLKELSITEEFK
                     LAKEIVKGMETDFNMDIPVDEVGYITMHIRGAKQRSSSNHKALNLDDIEIMEITNKMI
                     DLAEDEFKISLKNDERLFKDLANHLGPSINRLNMGLEIRNPLLDEIKSKYSYAYDGVE
                     KISRIIKDKLNINSIPESEIGYIAMHFASAIEKNLMMNTNINIVVACPTGIGTSRFLS
                     TKIENKFPNLNILETISAINIDEEYLKGKDVDLIVSTVELNTSLNYICVGPFMSLDDE
                     QIIKEKIKSIAQNKLINLNTKNDTKSKNKVYEQITESMNIGKDILQFLEEIRFEKFES
                     KDLSELIEDSSKIFAKSSEDIMSIKESLKERLKISIPYIEESKILLLHCMSERIDIMK
                     LSIIKLENRIVLDSNEEIDNVVFMLLPKNSPSYQRQIMSEISGSLIDNIIFTNKINKF
                     SIEEMTLEVKDIVFNFYTNRLKAFIDE"
     gene            57420..57842
                     /locus_tag="KR250_000809"
     CDS             57420..57842
                     /locus_tag="KR250_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBH0066774.1"
                     /translation="MSKVLNENNIFLGLDSVSKEEAITLAGRKLVENGYVKEEYIPAM
                     LEREKVMTTYMGMGVAIPHGVNEAKKEILSSGIVILQFPNGIDFDGEKAYLLIGIAGV
                     GDEHLEILSNIAVVLDDDLTERLKNSNDKQAFMEAFAK"
     gene            57861..59012
                     /locus_tag="KR250_000810"
     CDS             57861..59012
                     /locus_tag="KR250_000810"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBH0066775.1"
                     /translation="MKKAIQFGAGNIGRGFIGGLLVKSGYHVVFADVNEEILNSINKD
                     KKYTIHIRDVECIDEVIDNISAVSSIKEEIIDEIVQAEIITTAVGPLVLTKIASTIAK
                     GIKARKEKGLTSNLNIIACENAIYASSSLKEEVLKYLNKEEVEYLEIYVGFPNCSVDR
                     IVPPGKNENPLDVTVENFYEWNVEKQGFKGEIPTIVGMNLADNLMAYIERKLFTLNTG
                     HAITAYIGYLKGYKTIEESIKDKFICDIVKSAMVESGEGLIKKYNFDSEAHYKYIDKI
                     LNRFKNPYLNDDVLRVGREPLRKLSDKDRLIKPLMTAKSYGLSVDNLILGIGAALHYN
                     NSEDTQSVELQELIKSNGVKKAVAKIANISNDEELLNNIEKSYIFMKNL"
     regulatory      59118..59219
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            59402..59974
                     /locus_tag="KR250_000811"
     CDS             59402..59974
                     /locus_tag="KR250_000811"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HBH0066776.1"
                     /translation="MKALQEKILREGSVSGNDILKVDSFLNHQIDVAFLNEIGREFKE
                     RFKEEKVDKIFTIEASGIAIASIVSQYFDNAPVVFAKKSESKNLDKDVYETNVYSFTK
                     AREYSVKVSKKYINKGENILIVDDFLANGRAALGLKDLIEQAEANLVGVGIVIEKGFQ
                     AGGALLKANDVRLESLAVVESIDNGTVKFR"
     gene            60038..60688
                     /gene="fsa"
                     /locus_tag="KR250_000812"
     CDS             60038..60688
                     /gene="fsa"
                     /locus_tag="KR250_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HBH0066777.1"
                     /translation="MIFFIDTANIEEIKEANDLGVICGVTTNPSLIAKEGRDFIEVVK
                     EISEIVDGPISAEVISLEHEGMIEEAEKLSKIHKNIVIKIPMTAEGLKAVKVLSSKGI
                     KTNVTLIFSAGQALLAARAGATYVSPFVGRLDDISQNGLDLIEEIVDIFSVNSIEAQI
                     IVASVRNPIHVLQAARMGADIATVPLKVINQMIKHPLTDKGIDSFMKDWEGASLKL"
     gene            60840..63764
                     /locus_tag="KR250_000813"
     CDS             60840..63764
                     /locus_tag="KR250_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBH0066778.1"
                     /translation="MDKILREIQREDKKNPYTDQELAEMLNVARSEVISVRKKNNILD
                     SRERRKKILIKDISKIVSENPQMSERKITEILIENGYNISRSAVSKLLKEENLNQSKI
                     GIKDKEPASSGKYVKVSKVVEEDGFEELIGIKGSLKEKVNLAKSAIMYPPNGLHTIIY
                     GETGVGKSELATCMYKYAIKNNIKEENSPFIVFNCADYAENPNLLIAQLFGVVKGAYT
                     GADANREGLVEQANNGILFLDEIHRLPAAGQEILFSLIDRGEFRRLGESSSNRKANVL
                     IISATTENPDSNLLQTFRRRIPMVINLPNMSERPKSERYEIIIKFFEREAKRVNKNFI
                     ITKEVMCALMNYKCTGNIGQLKSDIQVTCARAFSKSIFSSDKVIIDLDSLRDYIKSGY
                     YDCNCKNEKYSEFSVEDIYIDISELVSGKNRILSDNEISEIYNYAEKELKVLESKCFS
                     EEELKNAFIEKLDKKFNAIKNNKNLSDRRSRIDWGGQLKESTLEIMDKVIVFIKEQFK
                     HVNQGLYLALAIHIEHAIGRIKDGKTIINPSLDKIKASMPMEYELSRYIMGIVEDLTQ
                     VTFPEDELGYLAYYINKFCYNEESIKDKVKVVIVTHGKVGIEMSKVVNHILGIECTLG
                     IEIALTDSPSEGIEHVLEELQKIEARKGILVLIDMGSLVILGDEVEKRLGIRCKTVNR
                     VDTLLAMEAGKLATIEGKSLDGIIADLKKNKNYAMINTNKFSYRKNEYGKKNVIITLC
                     LSGVGTALNLKEHIEKQIKEYDTLIEVKPVAFLNNNLEEELNDIEAEYNIIAICGTID
                     IDYKNVPFISYNEVLGKNGINKVLEHLNSKQPVLNIENQLNNLIHEDLILYNFEGISK
                     EYIIDTLVSKLEEGGYVDSKYILSVYKREAMGSVVMASKVAVPHGLPENVIKPAIAIA
                     RLNKPIVWDNKFMVDLVVLLALKENNKKEIRSLFSKINDQHTLEVLLNTEDKNEIKEL
                     LS"
     gene            64341..64799
                     /locus_tag="KR250_000814"
     CDS             64341..64799
                     /locus_tag="KR250_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBH0066779.1"
                     /translation="MEFINFVSDKVIDLKMDCKTKEDFFKEIHNKVFDLGFVKEEFGE
                     KILARENVFPTGLNLGDYGVAIPHTDAEYIKEQFISVCTFNEPVVFSSMEDQDEKVPV
                     NLAFVLGLNQPHSQLSVLTELMGIMQNKELVDKLMSSTDKEEVLKTLKSL"
     gene            64860..65135
                     /locus_tag="KR250_000815"
     CDS             64860..65135
                     /locus_tag="KR250_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBH0066780.1"
                     /translation="MKKILVACGAGIATSTVVCDKVERLVKENNIQAEVIQCKIAECA
                     TKQEGADLIVSTTILPTTYNIPAIKATAYITGVNTAALDKKILDALK"
     gene            65194..66468
                     /locus_tag="KR250_000816"
     CDS             65194..66468
                     /locus_tag="KR250_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012951111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBH0066781.1"
                     /translation="MDKLLAVVQYILNMGPTVILPITIVVLGMIFRIKFTKALKSGLT
                     IGIGFVGINLVVNLLTSTLGPAAQGMVERFGLNLTVIDVGWPTTASATWASPVAPILI
                     PVCLLVNLLLLYFNKTKILNVDIWNYWHFIVAGAVGYIVTGSYVWAIFCAILMELLVL
                     FIAEKTAKNVQEFYDLEGIALPTGSTASFAPLGYLVGKFVEKIPGINKIQADPDSIQK
                     RFGIFGEPMMMGIILGIILGALAGYDVAGIFNVGISMGAVMLLMPRMVRILMEGLIPI
                     SESIRDFLQAKYGDRELYIGLDAAVATGHPAVISTALILVPITLFLAVILPGNKVLPF
                     GDLATIPFYMAFIVCFRKGNIVQSVITGTIVIAISLYMATNFADVHTQLLQQANMMPE
                     GTTMVTSIDTGGNLLKWGILKLSQLVSSFGIF"
     gene            66598..67650
                     /locus_tag="KR250_000817"
     CDS             66598..67650
                     /locus_tag="KR250_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBH0066782.1"
                     /translation="MKAAVLHGTNDMRFEDIEIKPCESDEVKIKVMAAGICGSDPPRV
                     LKHWKYPVPAIPGHEFSGVIAEVGKDVKNVKVGDRVVAIPFIPCNECEYCKRGLFSLC
                     DDHGMLGAKSFGAFAEYVNIKATNVLPIGDMNFEDAAMIEPLAVAMHGVLNIGVQVGD
                     TVAVMGSGTMGQLVIQGLKIAGAGTIIAVDISDNKLRESKELGADIIINAKDINPVEK
                     IKELTGGKGVDIALECAGSKITQEQCLLITKKKSKIGFLGIAYSDITLSEEAFENIFR
                     KELELKGFWNSYSAPFPGQEWTKGINLVNEGKIKLKEMVSHRFSLEDTYKAFEMIRDR
                     KEEFNKILILPQGVEK"
     gene            67650..68681
                     /locus_tag="KR250_000818"
     CDS             67650..68681
                     /locus_tag="KR250_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HBH0066783.1"
                     /translation="MKAVVKTKPGYDNVEVLEVEEPKATGDKVKIKVEYSGICGSDIH
                     SFKGEYANIKAPVTLGHEFSGIVVEVGEDVKNIKVGDRVTSETTFATCEKCIYCETKD
                     YNLCPTRKGIGTQVNGSFAEYVLSREESIHVLPENVSLLSAALTEPLACCVHAALEKT
                     TIESSDTVLIIGPGPIGLLLAQVVKSQGATVIMSGVTKDKERLEIAKNLGVDRTVNVM
                     EESLADVVNEMTDGNGVNKGFDCSGFMPAVNEALRLLRKKGTFVQVGIFAKKLNEMDQ
                     EAIIQRELQYIGSRSQKPSSWITALDLMANGKVNTEALVTRTVGLDEFKEGITALMNG
                     EEIKVAVKS"
     gene            68773..69585
                     /locus_tag="KR250_000819"
     CDS             68773..69585
                     /locus_tag="KR250_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HBH0066784.1"
                     /translation="MSTLRDHSNNIRKNIIKMVAEAKSGHPGGSLSIADMLTVLYFDK
                     MNVCAENPKMADRDRFVLSKGHAAPALYATLAEKGFFPEEELITLRKFGSKLQGHPDM
                     KKVAGIDMSTGSLGQGLSAANGMALAGKLDNKDYTVYTILGDGEIQEGQIWEAAMTAS
                     HYKLDNLIAFVDLNGLQIDGSNEEVMNVSPVDDKFKSFGWNVIVINGHSFEEIGNAVD
                     EAKKVTGKPTVIIAKTVKGKGVSFMENNAAWHGTAPNAEQAEQALKDLEGGL"
     gene            69588..70508
                     /locus_tag="KR250_000820"
     CDS             69588..70508
                     /locus_tag="KR250_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HBH0066785.1"
                     /translation="MGIATREAYGQVLKELAENKDIVVLDADLGKATKSISFKEVAPD
                     RFFDMGIAEGDMIGTAAGLATCGKIPFASTFAIFAAGRGYEQIRNSVAYPNLNVKIAA
                     THAGVTVGEDGGSHQAIEDISLMRGIPNMVVLNPADALEARQAILTSIDYNGPVYIRL
                     GRAATPDVNSENYKFEIGKGTVLREGSDITVIATGIMVAKALEAAEELAKEGINVEVV
                     NISTIKPLDEALIKETAKKTGKVVTAEEHSIIGGLGSAVCEALAETKDVVVRRIGVKD
                     VFGQSGTPADLLKHYGLTTEDIVKNIKELL"
     gene            70683..71129
                     /gene="rpiB"
                     /locus_tag="KR250_000821"
     CDS             70683..71129
                     /gene="rpiB"
                     /locus_tag="KR250_000821"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HBH0066786.1"
                     /translation="MKISIGCDHGGYELKEYIKENLINKGIEVEDVGTNSKESVDFPI
                     YAKKVSQNVQSKKSDLGILCCGTGIGMSIAANKFKGIRAAVVSDCFSAKATREHNNTN
                     VLCLGERVIGKGLAMRIVEEWLNSSYNGERHARRLQMIDDIEKENL"
     gene            71159..71824
                     /locus_tag="KR250_000822"
     CDS             71159..71824
                     /locus_tag="KR250_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HBH0066787.1"
                     /translation="MSIICASIMCADQMKLKEELEALEEAGVKLLHCDVMDGIFVKNL
                     AMGPELLKSISENTTIPLDIHLATETPDKYIDMMSYIKPKYISFHVESSTNVKADIQK
                     LRNYGIGPVLAISPQTSVDKIEEYISLVEGILVMTVNPGFAGQKFNLSVLDKLDKLTE
                     ILKDYDNPPFIEVDGNINKDTIKLMNGKKVDIYVVGTSALFNDKPPISYKDKIEELKE
                     SIK"
     gene            71934..73436
                     /locus_tag="KR250_000823"
     CDS             71934..73436
                     /locus_tag="KR250_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HBH0066788.1"
                     /translation="MGDLYKLQNGTDIRGIAYENDSKEVNLTVEEVKKIAKAFHIWLK
                     EKTKKDKVTVAIGTDSRITGSQFRSTVIETLTNDDCNVIDCEIATTPAMFMTTIMDGY
                     NSDGAIMITASHLPYYYNGLKFFTESGGLEKTDIKEMLDIAVKNDSVDYEEVNKKGEV
                     IVKNLIEDYSNLLIDKIRKGVNSSKNYEKPFSGLKILVDAGNGAGGFFAEKVLHILGA
                     DTTGSQFLNPDGMFPNHIPNPENKEAMESICKAVLDNKSDLGIIFDTDVDRAAIVGKN
                     GKPINKNALIAVISSIVLEEHPKTAIVTDSITSEGLAKFINELQGRHHRFKRGYKNVI
                     NEAIRLNSEGEECHLAIETSGHAALKENYFLDDGAYLIAKILIKVAKLSLEGKTIEEL
                     IENLEEAQEEKEVRIGINKEDFRTYAEDILKDLDSHVKSIDGWSVAPNNFEGIRVNCD
                     ESNGDGWFLLRISLHEPLLALNIESNKIGGAEQIYSKLKLFLEKYDLKGL"
     gene            complement(73748..>74849)
                     /locus_tag="KR250_000824"
     CDS             complement(73748..>74849)
                     /locus_tag="KR250_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBH0066789.1"
                     /translation="YNNYNLKDMSITYVFFSNLLSMYNKKTINKKLAIVFLISIVVQA
                     IIQYLVLIFFMYIYYAEAIAIVILFTLVNTVFHFYIYNFLSVKFNYINNISKNIKNIE
                     NGNLKYKLEVIGDDEISTLAKSINNITGGLETAVDANIKNERMKAELITNVSHDLKTP
                     LTSILSYVDMLKNNDFDRETINDYINILDKKSQRLKLLIDDIFEASKLSSGDVEFNIT
                     KTDIRELLIQSIVEFEDKIQNSSLDFIIETPEYAVFTMIDGKKTWRVFENLICNILKY
                     SMPNTRVYIDMFIKEENIILTFKNISNDKLNLKPEELIERFRRGDISRKTDGSGLGLS
                     IAQNIIELENAHMEIIIDADLFKVMLTFRKVN"
BASE COUNT        29357 a         8245 c        13704 g        23543 t
ORIGIN      
        1 aaaaaatata tataaaagtt ttggagatgt ttttatatat aattctaaac tagattacat
       61 atataagttg tatggagatt tatatataaa atttggtaat atagatgaag gtataagttg
      121 ttatataaaa ggtttaaatt tagttaaaaa gagttcattg aatcttagaa aaatatctat
      181 attttatggg ctaatagcaa atggatatga gctaaaaaaa gattttaaac tggcaataga
      241 atattataaa agtatggatt ggtattctga taggtgggaa aaaaataatt cctataaaac
      301 atctcaagca attcacaaac aatatgaact cagacaaaaa caagagagtt taagattact
      361 agtggatgat aataagaaat tagaaaatga tattttaaaa gatggactta ctaaactata
      421 taatagaaga tatctggaac agcagttgga tttatttaat gaaaccagag acaaaaatat
      481 ggtcgcaata ctggtcgata tagattgttt taaaagttat aatgataatt atggtcatct
      541 agcaggagat aaagttatag ttaaggtaac tgatattata agaagtgtat ttatagatat
      601 taccgaacac ataatcagat atgggggaga agaaatttta atattagtag attctataga
      661 atcaatcggt gttgaagaga ttgataatat agaattatat attaaaagag ttttcaaact
      721 tcttgaattt gagggtattg aacatatata ttctaaaata aaaaactata taactataag
      781 tgctggagta agtataaaaa gatgtcgtag tagaaaagat gttgaaatat taattgaaga
      841 tgcagataag aacctatata aatcaaaaaa agcaggtaga aatcaataca ttataattta
      901 aaattgaggt gattttgttg cgaaaaaaaa taggaattat agcctctgat attgaattaa
      961 aagaaagaat agaagaatta tatagagaag atgtagaaaa tggaactata ataatcgaca
     1021 tacttaacct agacttaatg gaaaatcaag gtcgtatact tgttgaaaaa ggtgcccaag
     1081 ctataattgg tagaggtgga ggttatagtt tagtaataga tacagtaaat gtacctgtta
     1141 taccaatgaa tatgaagtca actgaccttt taagagcaat agaaatagca aagaaatatt
     1201 ctaaaaaagt agtattgatt cttggagaca atgaagtttc ttttgactat gttggctgga
     1261 gaaatgtcat aagtactgaa ataactgaag aatggtttga atctaagtat gagataagaa
     1321 gtaaagttgt aaaatatata gaccaaaaag atgaaattgt aatcgtagga ggaggtttgg
     1381 catgttcttt tgcaaggcaa tatggaattg atagtgtatt tgcaactgct agtgatgaat
     1441 ctataagaga agctgtagaa tattgtaaaa aattattaga tacattaggt gaagaaaagt
     1501 ttaataatga agtgttacga aatatacttg atggtataaa agatggagta attgctatag
     1561 atagtaatgg cagtataatt ttgtacaatg aaagtgcaaa aaacatgctt aaagtagaga
     1621 gaaaatgtgc acttaataaa tacatcttag atgtatttcc taaaatggag tggatgttag
     1681 attgtttaca tgaaaaagag gacgtagaag atagaaagat aagaaatata aataatctaa
     1741 ttgttaatac taggacaaca ttgataaaag ttgataatag tacttatgga gttttaggaa
     1801 taatacaaga tataaccaag ctacaaaact tagaaagaaa aataagattt gacttgaatc
     1861 aaaaaggtct ctatgctaga tatacttttg atgatttttt atttaaagat aaattaacta
     1921 aagaatttat tgaagaagct aaaaaaattg gaaaaagtga ttatacaact ttactttatg
     1981 gggaaagtgg gtctggtaaa gaaattatag ctcatagtat acacaatata agtaaaagaa
     2041 aagataggcc ttttgttgct ataaattgtg ctactatagc agaaaattta cttgaaagtg
     2101 aactttttgg ctatgaagaa ggtgcattta ctggtgctag aaagggtgga aaaagagggc
     2161 tatttgaact tgctcatgga gggactcttt ttttagatga aataaatagt ctatccttta
     2221 atattcagac aaaactatta agagtaatag aggaacgtca aataatgaga attggttctg
     2281 attatataat acctctggat ataaggatta tagcagcaac gaacgaatct ttaaccgaaa
     2341 aaatagttat gggtacattt agagcagact tattttatag attgagtagt ttagagataa
     2401 atatacctcc tttaagagac agaagagaag atataatacc tttgtttaac aattttgtaa
     2461 atgaagtatt aaaggatgat ggtctcaatg gaataaacag tatagatgaa aattttgttt
     2521 tgactaaaga tgaaatggat aagctatata attatagctg gcctggaaat gtaagagagt
     2581 taaagacaat agctcaaaaa tatgttgtga caggtaaaat taaattgaga caagatagag
     2641 attttaaaac taaaaaatta cttcctaact ctgaagtaga caaatttaat tctgaaacga
     2701 cagcaagtgt agaagtccaa gatgaatcta taaatataag taagataaat gatggtaaga
     2761 taagtataga tataaaagaa gtaaataagt atgttgaaga aaaaataata agtatgcttt
     2821 ttgctcaagg actttctaaa aatgaagttg cacaagttct tggaataagc aggacatctt
     2881 tatggaagaa atacaataaa aatatttaaa tataattaca tataaattat tttgaagtaa
     2941 tgttaatttt ttaaaacaag tgttaagaaa aattaacatt acttttttgc aaatttttat
     3001 ttttttagac aggcatatat atttataaga aaaatagtta aaacacttaa tttaataatg
     3061 aaaaaaataa taattctaaa caaaatcaat tttacataaa gtgggattta ttggcatagt
     3121 aattgcttaa tatataatgt aagagtaaat acaattttag gggtgatggt atgagtcaaa
     3181 gtatggttgg taagcatgca atgtggccaa aagaaaatga tgttatattt agcatatctg
     3241 gcagagctca agcagcagag aaggcttttg gtatggataa tgtaataaat gcaacaatag
     3301 gcgctctaat ggatgattca ggaaaattga taacaatgaa aactgtatat gaagaatata
     3361 aagcattaga taactgtgag ataggtgcat atgcagctct tgaaggtcaa ccagattact
     3421 tagaagcagt taaaaaagta tttttcagag attacttacc agaaggacat ataagagtat
     3481 tagcttcacc aggaggaagt ggtgcaataa aattagctgt atggaactat actaatgaag
     3541 gagatgaggt attaacatct gattggttct ggagtccata tgtaagtata gctgaagagg
     3601 ctaatagaaa agtagttaat taccaacttt ttgatgaaaa tagaaggttt aattttgagt
     3661 cgtttaaaga aaaatttgta aatatagcag aaaaacaagg tagagtcttt acaattataa
     3721 acacaccagc gcataatcca actggatata gtgttgcaga tgatgagtgg gataaaatat
     3781 tagacttatc aaaagaagtc gcaaaagata aagacaaaaa gataatattt ttcgtagatt
     3841 cagcatatat tgactttgca ggtgatgatg atgtatgtag aaagtttttt aagaaatttt
     3901 ctaatttgcc tgaaaacgtt ctcgtattgg ttggattcag tatgtcaaaa ggatttacag
     3961 cttatggtat gagaatgggg gctataattt gtatatcttc aagtgaagat gtagctgaag
     4021 agttccatta ctcatgtgtt cattcatgta gagcaaattg gtcaaattgt aatagaagtg
     4081 ccatggcagt attatctaac attgtaaatg accctaaaaa attcaaagaa tatgaggatg
     4141 aaaaagaaat atacaaaaac atgcttacta gaagagcaga tgtatttgta aaagaagctg
     4201 aaagagtagg attagaaatt cttccatata tagcaggttt ctttgtaagt ataccatgtg
     4261 ataatccaaa agaagtatgt gaagaattga ctaaacataa tttatttgca gttccattaa
     4321 aaatgggttt aagatttgca gtttgtgcag tttctgaaga taaatgtaag aaagcaccaa
     4381 gtattataaa agaagcactt gaaagtttag aagtaaaaat aaataattaa tttcattgaa
     4441 caaattgggt ggactatgaa tttaataatt gtagatggag ggaattttat atgaaacaat
     4501 tgatgacagg taatgaagca attgcacgtg gagcctatga ggctggtgtt aaatatgcat
     4561 ctgcatatcc agggactcca agtacagaaa tattagaaaa tatagcaaca tataaagatg
     4621 caatagttgc agagtgggct ccaaacgaaa aggtagcact tgaggctgct atagggggtt
     4681 caattgcagg agcaagaaca atggcttcta tgaaacatgt tggtttgaat gttgcagctg
     4741 atccaatatt tacttatgca tatacagggg taaatggtgg tatggtactt attacagcag
     4801 atgagccggg aatgcattca tctcaaaatg agcaagacaa taggatgtat gcaaaatttg
     4861 caaagatacc tttatttgag ccatcaacta gtcaagaagc taaagatatg ataaaagaag
     4921 cttttgaagt tagtgaaaaa tacgatacac cagttttata tagagttaca acaagacttt
     4981 gtcattcaaa aggattagtg gagtgctatg atagagaaga agttgaaatt aaagagtatg
     5041 ttaaaaatgc taagaaaatg gtaactgttc cagcaaatgc tcaaataaga aggggcgttg
     5101 ttgaagaaag aatggaaatt cttaaaaaat tctcaaatga aactgattta aactactatg
     5161 aaataaatga tacaaaaata ggtgttatag cctctggtat gtgctgtaat tttgccaaag
     5221 aagtttttgg aaaaaatgca tcatatatga aactaggatt tacaaatcca ttaccttatg
     5281 aaaaaataaa agaatttgca gaaaaagttg ataaaatata tgttatagaa gaaaatgacc
     5341 catttataga agaacaaata aaagcatatg gaatagattg tattggaaaa gatgtaatac
     5401 caccatatgg agaaatgaca ccagatgttt taagaaaagc tatatttgga aaaacaaatg
     5461 atactataga atacaaatct gaattagtaa caccaagacc accaagtttc tgtgcaggat
     5521 gtccacatag aggattcttc tatgaacttg gaaagagaaa aaatttaata gttggtggag
     5581 atataggttg ttatactctt ggatttgctc ctccatataa tggaatagat tatgttgttt
     5641 gtatgggttc tgcttttgga actgcacatg gagctcaaaa agttcttaat atgaaggatg
     5701 ataatgaaaa aagattggta ggtgtacttg gagactcaac attcttccat acaggaataa
     5761 atggactttt agatgttgtt tacaatagag gtaattctat ttcggttata ttagataata
     5821 gaataactgg aatgacaggt caccaagaaa acccaggttc aggatatact ttacaaggtg
     5881 ctaaaactaa agaagttgat atagaaggat tagttaaagc ttgtggaata gaacatgtaa
     5941 gagttataaa ccctaataat ttaaaagaag taaatgaagc actagattgg gctttagcta
     6001 tagaagatga accatcagta ataataacaa gatggccatg tgtacttaaa aaattctcta
     6061 aagaagatat agaagaattt aataatccat ttaagactaa gtgtaaagtt gaccatgata
     6121 aatgtatagg atgtaaatta tgtttgaaaa ctggatgtcc agctctatca tttgataaag
     6181 agaataagtt atcaaatata gatagaaatc agtgtgtagg atgtggagta tgtgcacaag
     6241 tttgtccaaa acaagcaata gttaaggagg aaaaataata tgactaagag tatacttttg
     6301 gtaggtgtag gaggtcaagg aactatactt gctagtaaat tattaactat gggattgatg
     6361 gaagctggat atgatgtaaa gatgagtgaa atacatggaa tgagtcaaag aggaggttca
     6421 gtttcctcac aagttagata tggagattgt gttcattctc cagttataga aattggtgga
     6481 gcagatatat tagtatcttt tgaaaaaatg gaagctttaa gatggttcaa ttatttaaaa
     6541 ccagaaggaa aagctttagt aaataatcat aggatagatt ctatgactgt tttaatcgga
     6601 ggagctgaat atcaagaaag tgatatagat gcagagttaa gtagattaaa tgcaaaagtt
     6661 ataaatgcag ctgataaagc tgaagaacta ggaaattcaa aaataatgaa tgttatttta
     6721 ttaggatgtc tagttaaatc tatgcaatta gagtctattg attgggaaaa aattataagt
     6781 gaaaatgtta aaccaaaatt tgttgaatta aacataaaag ctttccatga aggtatggaa
     6841 atggttagca aatagtttat aatagatata aatagactat agaaattaag gtgaaaggaa
     6901 gaaaattatg acttacagaa tattagccat aaatccaggt tctacttcta caaaaatagc
     6961 agtatatgat ggagaagaac aaattcttgt gaagacgata gaccatccag ctgaagagat
     7021 tgcaaaatat aatactatac aagaccagtt tgaaatgcgt aaggaagcag ttttgaatat
     7081 tcttaaagaa aatagtatag acttaaaatc tcttagtgca atagtaggaa gaggtggagt
     7141 tttaccacca gtaaaatcag gagcatattt agtaaatgaa gaaatgattg atgtactaag
     7201 acatagacca gtacttgaac acgcttccaa tttaggtgct gttgtggcac atgcaatatc
     7261 agaacctctt ggaatcaact catatattta tgattctgtt gcagtagatg agcttataga
     7321 tgtagcgaga atatctggac tttgtggaat ggatagatca agtgcagggc atgcattaaa
     7381 tactagagca atggctttaa aatatgctaa ggataaagga aaagattata agagcttaaa
     7441 cttaatagta gctcacattg gtggaggagt aagtatttat cttcatgaaa aaggaagaat
     7501 ggttgatatg ctatctgatg atgaaggacc attttctcca gaaaggtcag gaagagtacc
     7561 tgctacaaaa ttagtggctg cctgttattc aggtcaatat tcagaaagag aaatgactaa
     7621 aaagataaga ggtaaaggtg gtatagtttc atacctaaat actgtagatg ctagagaagt
     7681 tgaaaaaatg atagcagaag gaaatgaaga agcaaaaatt atttatgaag caatggctta
     7741 tcagttagca aaaggtattg gagagttagc aactgtagta gatggaaagg tagatgctat
     7801 aattataaca ggtggaattg catattctga aatgtttact tcaatggtta aaaagaaagt
     7861 tgagtttata gcaccagtag aaattatggc aggagaaaat gagttggaat cacttgcttt
     7921 tggaacttta agagtactaa atggagaaga agaagctaga atttatagtg aaaattaata
     7981 agaaaaacta aaaatgttta tatactaaaa aagtggtata tccgtttttt atacacgaaa
     8041 gcttagagta taggtgaaaa tgtataaaat ttggattgcc actttttata attaaaattt
     8101 tatcttaacc taataaataa ttctgtaaaa tttcgtattt atatatatat tttattggta
     8161 tttattgtta ttattattat gaggagtgat ttttgatggg aaaaataagt ttaaaaagta
     8221 tgataagatt attttcaggt ttttttatat ttgcaatttc ttctgtgctt atgattaatg
     8281 cacatgtagg tcttatgcct tgggatgtac ttcaccaagg tttatctata aagctaggca
     8341 taactatagg tcaggcttct ataatggtag gagttgtaat tgttatattg gatgcagttt
     8401 ttggtgaaaa tataggatgg ggtactttac tcaacatgac atttatagga atttttattg
     8461 atttagtaat attttctgga gtgatacctc atgcaagcaa cacttatata ggtgtgttta
     8521 tggttgttat aggaattatt ttggcagcta tagcaagttt tctatactta ggtgtttgtt
     8581 taggaagtgg accgagagat gggcttatga ttgctcttca gaaaaaaact aataaatctg
     8641 tacgtcttgt tagaacaatt cttgaaatac tagcattagt tgttggatgg ttattaggag
     8701 gttctgtagg tattggtacg cttgtaagtg ctttaggttt aggatatgta ttacaaatag
     8761 tgtttagaat atttaaattt gacacaaaat tgctgaaaca tagatttatt atagatgata
     8821 taagagaatg gaaggaaaag aaaagcaatg aacataaatg taaatcaagt attgtaataa
     8881 aaaatgagca gaattaatta tgctaaaata tatttgtagt ttatctaatt tatagtaaag
     8941 tcaaggagat tttgaaatgg aattatggga tttatataat gctgatggaa ttaaaacagg
     9001 aaatgttata aaaagaggta attctattga agaaggttat taccatttgg cagttgaagt
     9061 gtggatttta aatagtaact ctcaaatttt aattcagaaa aggtctaaaa gtaaaaaaac
     9121 attgccaaat atgtggggaa tgacaactgg atgtattgtt tctggggaag acagtttaga
     9181 aggagcaatt cgcgaagcaa aggaagaaat agggatagat ataactaaag atgagatgaa
     9241 agtttttaga agtatgattc atgaagatac tctttgggat gtatacttag ttaaaaaaga
     9301 atatgatata tctaaagcta ttttacagga ggaagaagta agtgatataa agtgggtaag
     9361 cacagatgaa ataagacaat tactaaaaga gggtttgttt tttgaatatc cagagatata
     9421 cgaattgcta tatgagatag ataataataa attaaatata tgatgtagag cagatttcag
     9481 tataagtaag attatttaaa atgtatcttc tctaaatttt aatattgttt tatgaaaatt
     9541 aataagttat ttttagtatt tttctacttg ttttttgaga ttaaaataaa aagggcagtc
     9601 attataaagt cataaaaatt tggggaggat ttaaactatg gaagataaca aaggattggc
     9661 acgtgacagt tcaaaatata aaattgtttc tatatctgtc gtagtaatta ttctaattac
     9721 attttggtat atgttaaata tggtgctttt aacatttatt atgactttta ttttctataa
     9781 cttgcttgta gccacaagaa aacgaattaa gaagttttct tctttaaata ttccagattc
     9841 attgataatt atagtgttat atgcactatt tgctatattg ttagtattga ttagctatgc
     9901 agttgtacca ataattatag ttcaacttac agagttgagt agggttttta gtgattttga
     9961 tgtaaatcaa tttgctcaat ctttaggacc gaaattgtat cctattgtct caaagctgga
    10021 ttttaataaa tatatttcac aagcagggct acttatagca tcaacagcaa caaaagttgg
    10081 aagttttgga gtaaatatat tattggcatt tttactgagt ttactccttt tacttgaaaa
    10141 aaatgaaatt aaaaattttg gagataaact ttctgatagt aagatttctt ttatttataa
    10201 ttcgcttgta ttttttggaa agagctttgt taaaaatttt ggtgaagtaa tgaaagtaca
    10261 agttatgatt gcctttataa attctgttgt ttctatgatt ttcttaggtt tcatgggatt
    10321 ccctcaaata tgggctttag gatttatgat ttttgtgttg ggtcttattc cagtagcagg
    10381 agttatagtg tctttgattc cattaactgt aatagcattt aatacaggag gaataacaaa
    10441 ggtttttgga gttttactta tgatttgtat agtgcatgca gtagaaacat acatattaaa
    10501 ccctaagttg atgtcgaata gaactaaact tccagtttgt tttgttttta ttattttact
    10561 tgtaggagag cattatttag gtgtatgggg attactaatt ggagttccaa tatttatgtt
    10621 ccttatggat attctgggtg ttaaatttac ttctaggtaa tatattaaaa taataattta
    10681 tttttatata taaattatga attgttttaa aaaagaacct ataaatagac atcattaata
    10741 aagacatcta tttgataggt tcaattttgc tttgtataaa tactataaaa ctaaataaaa
    10801 agaaatttat ttcatttcaa aagtttcaca acttgcattt ctttcattta tatgaacttt
    10861 ttcagctttg caaagttcat ttttattgta tttacatttt acagcttcac aatgaatgtt
    10921 atctgtatca acttggttag tttcattaga acgactagtt ataccagatg tatatttgtc
    10981 ttcaaaagaa gcacaataag tattttctgt agtagttgct tgcattccat ctacttttat
    11041 actaccagca taacaatatc cactagtatt ataagcacaa ttagtagcag cacaatttaa
    11101 attattattt ttcataaata acctcctaag ttttatttaa acttattatg tgaagttatc
    11161 taaattttat acacttattt acaattacat ttatcacatt taacaactac atctttggaa
    11221 gtttcccttc ctaaattttt attaacatga tacacatcaa cattttcagt tgatccacat
    11281 atagaacatt taaaattgtt ttctagtagg gttctttctt taaataactt ccaatacata
    11341 gaattgatgt aatcattata atttaccttg aggcatgtct caagaaagtt tatgtataga
    11401 ttgtctaaat ctcctgtatt taattctgta tattctggtt caaaatattc acaacatcct
    11461 ctaaaatcgg gctttgtatc tgaaataaca catctaatat catatctata gtctaggcta
    11521 ggccccttaa atgaaagatg tgtgcaaaaa gaacaagata tttctggtag atttttttca
    11581 agttcatgta atttattatt cataagtatt cctccaaaaa atttatttta agtatataaa
    11641 tgaatagatt attttaaaac ttttatttat ttatattaga gatttaggat aaatattcca
    11701 ataaggagta aatacttaag tatttattac tgtattaaat aaaaaagagg caaatctaac
    11761 ttgagattgc ctactttttt atcatttttt aataaaattt attctaagta ttattttatt
    11821 tagatttagt cagccaactt agtatctgct ttgcttttta actttctacc agagcgtaca
    11881 gttaaaatag catataatac agtaaatatg gcagctaaaa catatgatat atttatgttt
    11941 aagttaaatc caatttgagc attaaatata taactgaata taacaaagga ataaaacata
    12001 ccaggaatta agcttataat ataattcttt tcttttatta taaggtaaac tgtaatcatt
    12061 gcaaatgcaa atatggcaat agtttgatta ctccaagaga agtatctcca caatatatta
    12121 aatccagaag cactaagctt tgcaaatata aggatggcta tgacaggaat aaatatacag
    12181 attgacaata taacacgatg tttcttttcc ttttggtcat aatgaaagta atctgcaagc
    12241 attaatctta aagatcttaa agctgtatct ccagaagtta ttggtaaaac aattacacct
    12301 attatagcta tgataccacc aatagaacca agtaaatctc ttgcaaccaa acctattaca
    12361 tcaggagaac ctactaattc tttaggtatt cctttattgt aaatacccat agcagctgca
    12421 gcccaaatca ttgcaattaa accttctaaa atcatcatat tataaaaagt agtttttcct
    12481 tctttttcat ttgaaactga tcttgctata agagtagctt gagttgagtg gaatccagaa
    12541 acaataccac aagcaactgt aacaaagaat gtaggaataa gaggaatacc atcaggatga
    12601 atacctttcc aatttgcaag acttaaatta gctaaatcat aaccttgtgt aaagattcca
    12661 attccaacac cagcagcaga taaaagtaga atagctccaa atattggata tacctttcct
    12721 atgattttat caattggaaa tagtgttgca cacaagtaat atagaaagat aagtccataa
    12781 actatccaga caactggatt atttatagtc gatttcatat ttaaaatttg agtaacaact
    12841 aaatccccag gtgtataaat aaaaactgca ccaactaaaa gcattaaaag acaaaggaat
    12901 atattataga cttgaaatac tttattacct aaaaatctac tgataagaga aggcatttgt
    12961 gctccttttt ctcttatact aatcattcca ctcatatagt catgcaaagc tcctccaaat
    13021 acacagccga taggtattag tatgaatgct attggaccaa acaatatacc ttggataggt
    13081 cctagaattg gtcctgtacc agctatatta agcaactcaa ttagactatt tttccatttt
    13141 ttcattggga catagtctac tccatcactt tgagcaagtg caggtgtttt tctgtcatct
    13201 gggccaaata ctttttcaca ataagcacca tataaagcac ctcctattag taatatagcc
    13261 aagccgatta agaatgttgt cataatatca ccccatttat aaaatttttt ggtctttgta
    13321 tacatattat ctaaatgtgt agatttatat tgaatttaat tctggacaac ctacaaattc
    13381 tataaagtat tacaactaat ataatttcag aaataaatac atatacataa ccatgtactt
    13441 agtatacagg tatttggtaa tattttcaat gttatagcaa taaagaatat aaacaaaatt
    13501 ctgataatat tttacaaaaa aattaaaaaa tataaaaatt agtttaaata gatggagaaa
    13561 aaaggaaaat caattgagaa tataaatgtc tatatcttaa caatattaag aggtttaaaa
    13621 ttcttgaaga aagaatagtc aagtgatata atgggtttac aggtgtatat acactagtac
    13681 atatagaaag cggtgcaatt atggataaag atttaacata tcaaatcaaa gagctaaaga
    13741 aagaaaaaaa tgcaataata cttgcccatt tttatcaacc accagaaata caagaattag
    13801 cagatgcagt tggtgactca tattatttaa gtgagattgc tagagattgt aaagaagaag
    13861 tggttgtatt ttgtggagta aggtttatgg gagaaagtgc taagatatta tcacctgaaa
    13921 aaactgtatt gatgccagta agtaacgctg ggtgtgctat ggcagatatg gtagatgaag
    13981 aaggcgtgat aaaattaaag caacagtatc caaatgcttt ggttgtatgt tatataaact
    14041 ctacagctaa agttaaggca cattgtgatg tttctgttac atcttctagt gcaattaaaa
    14101 tactagaaaa catagataat aaagaaatta tattccttcc agataaaaat ttaggaggat
    14161 atattgcaga acaatttcca gataaaaact ttatattttg ggacggatat tgtaaatacc
    14221 ataacaatat aagagctgaa gaaataattg agttaaaaga taagtataaa aatgcagaag
    14281 tattagtaca tccagaatgt aaaaaagaaa taagagattt aggagattat gtaggtagta
    14341 catcaggaat aataaaatat gctactaata gtaaaaacaa agactttata atagcaactg
    14401 aagaaggtat attacatgaa ttaaagaaaa ataatcctaa taaaaacttt tatattcctg
    14461 gtggaaaaat actatgtaca gatatgaaaa agacaacatt agaaaattta tatagtacac
    14521 ttaaaaatat ggaaaatgaa gttattgtag aagatgaaat catggaaaag gctttaaatt
    14581 cactgcttaa catgcacaaa ttagcagaag gatagaggga tagacttatg aatttagaac
    14641 aagatgtttt aatagttgga agtggagttt caggattata ctgtgcatta aatttagata
    14701 aaagtttaaa tgtgctagta gtatcgaaat caacaattga aaacaacaat acatatcttg
    14761 cacaaggtgg aatttcaact gctagaaata ttgatgatat agaatccttt gtagaagata
    14821 caatgaaggc tgggcagtat aaaaatagag ttgaagcagt tcaagtactt gcagatgaat
    14881 ctatagaaaa tgtaggtcag atagtagaat atggtatgcc tttagacaaa gaaaatggag
    14941 agatagatta cacaagagaa ggagcacata gtgtaaatcg aatagttcat agtaaggata
    15001 atactggaga agttgttttt aaaactttat taaaagaagc taaaactaga gaaaatataa
    15061 ctcttataga agatgcatat cttttagata tattaaaaga tggcaataaa tgtataggag
    15121 ctagaatttt taaatctaaa aaagagattc atgttttttc aaaaatagta gttttagcta
    15181 caggtggaat tggtgggctt tttaaaaatt ctactaatca aagacatctt acaggtgatg
    15241 gaattgcaat agctttaaga aataatataa aaatagaaaa tttagaatat attcaaatac
    15301 atcctacagc cttttatgaa gaaaataatg aaggtaggag aatgcttata tctgaatcat
    15361 taagaggaga gggagctaaa cttttaaata aaaataaaga aagatttgtg gatgaacttc
    15421 ttcctagaga tgtagtttca aaagctattt ttgagcaaat ggaaaaagat aaattacctt
    15481 atgtgtattt agatgcaaca catttagata gtgaatattt aattaataga ttttctttta
    15541 tttataatga atgtttagct agaggaacag acataacgaa agaatgtata aaggtatctc
    15601 ctgcacaaca ttattttatg ggcgggatac atgtagattt ggattcaaaa acatctatgg
    15661 aaaatttata tgcagttggt gaaattagtt gtacaggtgt tcatggagcc aatagattgg
    15721 caagtaattc attacttgaa gggcttgtat tttcaaaaag agcagctaaa aatataaact
    15781 cagttataga taatgtaaaa gttaagttta tagatgtacc agatatggat atagatatag
    15841 aacaggtaaa aaaagaaaat aaaatattag tcataaaaga aattgaaaga acaagtgagg
    15901 attttggcga tgaattattt gattattgat agaatgatac aggatgcttt gatagaagat
    15961 gttccaagtg aagatataac aactaattct attgtggatg aaaattctaa atctactgta
    16021 gatttaattt gcaaacaaga tggtataata gctggcttag gtgtatttaa gagagttttt
    16081 gagatacttg gtgatgtaga tgtaaaatta tataaaaatg atggtgatag agtaaagaat
    16141 cgtgaaaaga tagctgtatt aacaggtagt actagaaact tacttgtagg agagcgtgta
    16201 gcattgaatt atttacagag aatgagtggt atagctacta ttacaagtag gtatgttgaa
    16261 aagcttgaag gtacaaatac aaaattatta gatagtagaa aaacgatacc taatcttaga
    16321 atattagata agtattctgt caaagtaggt ggaggatgca atcatagatt taatttatct
    16381 gatggaatat tgcttaaaga caaccatatt ggggctgctg gtagtgttaa gaaggcagtt
    16441 gaattagcta gaaaaaatac ttcttttgtt agaaagatag aagtggaagt ggaaacttta
    16501 gacatggtaa aagaagcaat agaagccaat gcagatataa taatgttgga taatatgagt
    16561 ttggaaatgg caaaacaagc agtagatatt ataaatggaa gagccattgt agaattttcc
    16621 ggaaatgtaa atttagatac tatagaagat ataggaaaaa ttggtgtgga tgttgtttct
    16681 gtaggagcgc ttacacattc tgttaaagct ctcgatataa gtatgaagaa tttgagaaat
    16741 atataaattt tatattgaaa taaacttaat taaaatagac tccttaaatt tgttttataa
    16801 atttaaggag tctatttttt attcattaaa attaataaaa atagaaaaaa atcaatcaat
    16861 aatttaataa aaatagataa atactatata cacttttaat aatatagtta atatctattt
    16921 tacagataaa taagaataaa cttataatat aattgtatct gtaaaatttt gtaaaatata
    16981 aaaagtatag ggggaataaa aatgaaagta gcttatattt ttagtagtac taatacacat
    17041 aaaattctag acaaaatgat aataccacaa cttgaacaag gaactcatgg agtagacgtt
    17101 ctagggatgt ttttctttat ggataatacc ttatttttat ctaaaggtaa tcctgtaggc
    17161 gagagactaa gtaaattaca tgaaaaaact gatatgataa taatggcttg tgaccaatgt
    17221 gctatagaaa gaggtataga gaataatttg gttgatggag ctacaattgg atgtttccca
    17281 aacctatatg cttgtttagg cagtgcaggt gtggaccaag ttataacgtt ataaattagt
    17341 ataggataca atccatagaa aaatttattt tctgtggatt attttaagta aaagataaat
    17401 tgtttaaagt aggggatgaa caaatgtcta aaattttagt tgaatttata aacacttgac
    17461 catcttgtga tgaacatgga gtgtttataa aaaaaatgtc agaaaagtat gctgataaat
    17521 tggaaataaa attatatcaa gcagggaaag atttttcgta tataaaaaaa tatgggatta
    17581 taacaaaagg aactttaata ataaatcaga aaaagaaata tgatagatta aataaagata
    17641 caattgaaag ggcaatagta gaagctataa acaataatta aaagtttaaa aattaggagt
    17701 atcaaaaatg aatatattag aaattgtttt aaatggagtt aaagatacta tagtagcttc
    17761 tatagttaca ctgaatggag catcaggatg gcttatgata agttttatct tggcaggtgt
    17821 acttcacaat gttttaactc caagaaggtt tcaaaaacaa cttggaaata agaaattttc
    17881 ttctttaatt aaaagtacta tctcaggaat gtttttacca atatgtagtt gtggagtaat
    17941 accacttgga ataagtatgt attattcagg agcatattta ggaccagtac ttgcttttat
    18001 gacatcaact ccaataataa atccaatagc tgtagttctt tgtctaggtc ttttaggtcc
    18061 taaagttaca atcatttatc taatagctgg ttttttagta ccatttttag taggattttt
    18121 aggaaattgt tttggaaaag aagaattatc acttcataat gattcagagg aagaagtaat
    18181 agaattagaa gaagaaaaaa gaagttttat ggaaaatatg attgatggtc ttaaatggtc
    18241 ttttggtgaa ttagccctta ctattagtaa atatgtagta atgggtatgg tttttgcagg
    18301 atttataaca actatttttc caaatagtat aattcaaaaa tatttaggaa atccaggttt
    18361 gctatctctt ggaagtatag caatacttgc atgtgttatg tatgtatgtg ctgtaggaca
    18421 tataccgttt atagcagccc ttgttgcaag tggagcatct cctggtatag cgataacatt
    18481 tctaatagct ggagcagcta caaatctacc agaacttata agtatgttta aacttattgg
    18541 aaaaagaact gttattatat attctgtaac tttgacactg tcttcaataa taattggata
    18601 tataacaaat ttacttttag tgaataataa ctcagtaaat ttagatgcaa caaattcttc
    18661 aatacagttg gcaaataaac taatgttaaa tatacctgat agttttgagt atgtatgtac
    18721 tattttaatt attttatttg catttaaatc aataatacca aagatgaaag aggtcatagg
    18781 aaatcatgct taaaaagata gttataatat cctgcatagt agttgtgtta atcattttat
    18841 caaaaatagt tgatgataac atcaaagaag atgcttctat tccaaatgtg aataaagaaa
    18901 cattagaata ttttagaaaa aattataaag aagacataat aacttgtgct gaagaagact
    18961 taaataatga tgggaaaaaa gatttagtag ttatatataa aaaaagcaat aattcaaatg
    19021 aaatggtagt cgtagtttct gataaaaata gccactatat aacaaagcca ataccagcac
    19081 ctatagagaa tcaaactata acctttaaaa atattgatga taaagcacca atagaagtaa
    19141 ttgtatcagg gtctaaaaat ggtaatgtag gatatgctat ttacagagta gagggtaaaa
    19201 aatttgttga tttatttggc gaagatatgg acaaatgttg ctaaaaataa aaataataca
    19261 taaaggtggg aaatttatat gaaattatct aagaagattc aagcattaat cttatttggt
    19321 gtaatgggta catctatatt aacaggatgt agtagtaaac caaaagaaaa agaagaagca
    19381 aaaactggaa aagatgatta tacaataaca gtaggttatt ataattgtga ccatatgact
    19441 gcgggaccag tagcagaatc ggcaggaata tataaagatt taggtcttaa tgtaaaaact
    19501 gttggaaatg gtaaagttcc tgaagctatg gcagcaggaa aaatggatgc aggttacata
    19561 ggaactaagg ggttagttgg agcaatacct aagggttcac caataacaat agcagctaac
    19621 aaccatactg gtggttctga atatttaata gtttcaaaag atataaaaga acctaaagat
    19681 ttaataggta aaaaaattgc aacagatatg agtgatttct tatggacaag tgattatggt
    19741 ccagaaacag gtctaccaac tgacccatca aaatatgaag ttgtaaatat ggattcagac
    19801 aaagataaat atttagctct taaaactggg aaaattcaag cttttacatc ttgtgaccct
    19861 tggggttctg tagctgaaaa tgacggtgca ggaaaaataa tagcatctac tcaatataaa
    19921 gaaaaagcta atggcaaaga atataactgt tgttcattct cattaaacaa aaactttata
    19981 aaagaacatc cagatttagc aaaaaaattg gttctagcac atacaaaagc aatagaatat
    20041 atatatacaa atccagctga agcagctaaa atatttgcaa aatattataa tgtagaagaa
    20101 gaagttgcac ttagaactat ctataagaag actgttggag aaggtagaac tcttacttgg
    20161 aaagttacag gtgaagaata taaaaataat ttacaaatgt ataaagattt aaaggcttta
    20221 gatgatatac ctaaatatga agatacgatt gatacaagct tattggactc ttgtggagct
    20281 gatgattttg acaaatttat aaaagaaaaa gttaacccag agtttcctga aggtatgagt
    20341 tatgaagact ggaaagcaaa ggtattataa ataaatggag aatatgaaaa atataataat
    20401 actttttatc attattttgg gaagtatatt tataatattt tttaattttg aagataaaga
    20461 agaaataaaa ggaaaattaa gaataggggt atctgatgat acctctggtt ttgtaataaa
    20521 ttatatggta aatagagact actttgaaaa tataaaagta agtgatataa tggatatttt
    20581 tacaattaat gattgctgag caagtacaat gcagtgggct ctgagttcag aggaaataga
    20641 tatagcaatc atatgtaaag atgctgcaaa acaatatgta aatgtaaata gtggatttga
    20701 aattgttggt actgtagttc aaaattctga tatatttcta ttaagtgata ataatcctga
    20761 aaagatagga gtcattcaaa atagagatta ccagtcagaa ttggttaaaa aatactacaa
    20821 gaatgtggaa gtagctccct tattagggac tgcactagca tatggattag aaagtaattt
    20881 agtagatggt gttgtaatag attgcataaa gtcaattggg ttagagggca aaagaaaatc
    20941 tacaactaaa ttaggagatt atgataccta tgtattagtt gtaagtaaag agtttaaaaa
    21001 aagtaaagca tatagtgatt ttataacact ttacaataaa agtgttgatg ctttaaagaa
    21061 taaagctaca tttaagaaag cattaaaaga gtacaggaat ataagaaata tagatatgga
    21121 ggaaataagc aattggaaat taaaatttct aaagataaaa gcgatagatt aactaagaaa
    21181 aaaactgatg gtagtatgga tattttatat aaaataatac tattagttat tatattttta
    21241 gtctggcatt ttgcagcaaa agatataggg agttctttgt tacttccaat gccagtagat
    21301 gtaataaaag gtttcttttt ctgtgtgaca gatgcagaga cagtcactaa tctttttata
    21361 acattgcaga gagtacttaa aggttttatg tatgcattat tgtttgggct tccaatagga
    21421 tttattatgg gattttctaa aacttttgaa agggttttat ctccagtagt agattctgta
    21481 aggcaagttc caataatggc atgggttcct cttactatag tatggtttgg tataggagat
    21541 ggtccaacaa tatttttaat agctttttca ggggttttcc ctataatttt aaatactatt
    21601 caaggagtaa gagcaatatc aaaggattat tataatgcag ctagaagtat gggagcatcg
    21661 ccaattgtga tatttacaaa tgtaattgtt cccgcatctc ttccagatat actaactggt
    21721 tcaaggattg ctataagtac aggatggatg tctgttatct gagccgagtt tatagctacg
    21781 agtgccggtc tcggttactc aatggtacaa gctcaaacat taatgcatac agatgtatta
    21841 attgggctta tgatatttgc agctttaata ggatttataa tagatagggt attaaagttt
    21901 ataaataaaa tcctttgtag atggaggttt gcagattaat gaagttaagt gtaagagata
    21961 taaataagac atttgtaaat aatagagtac atacaaaggt tttagaagat ataagtatgg
    22021 atatagatga tggacaattt gtctgtcttt taggaccatc aggttgtgga aaaacaactt
    22081 tacttactat aataggtggg tttcaaaaat cagaacgtgg agatgttttt ataaatgata
    22141 aaagagttaa aaaaccgggc atagatagag cttttatatt tcaaaattat gctttatttc
    22201 cttggaaaac tataagaggt aatgttctat ttcctatgaa acagcagaaa ataccaaaag
    22261 aaaaaagaga agaaatgcta gaagaacttt tggttatgtc agacttgaaa ggaaaagaaa
    22321 aactgtttcc gcatcaatta tctggaggaa tgaaacaaag agtggccatg ataagagctt
    22381 tagcatgtaa tccagaggta ttactaatgg atgaaccatt aggagctgta gattttcaaa
    22441 tgagacaaaa tttacaggaa gagctagaac gaatatggat aaagaagaaa ataactgcat
    22501 taatggtaac tcatgatgtt gatgaagctg tatatatgag tgatagagtt atagtaatgt
    22561 cgagagataa aggaagaatt attgatgata taaatataga tattccaaga cctagaatta
    22621 gaggaagcca aaaatatgaa gagtacaaaa acaaattaac tgatacttta tcaaagtgtt
    22681 atgaagttta aagaggagta gacatgagtc aaatagaaaa atacaatagt ttggataata
    22741 cacaacagaa aataagaatc tctattatgg atatgataat agataaaggt tctagtgtta
    22801 cacttcaaga agtgactgaa tatataagta aaaagttgaa tatagaaaaa gaatatatag
    22861 aaaggacact acaatatttt atttataaaa atataatggt tgttgatggg aatagtataa
    22921 attttatata tcctgtatca gcattgccaa caaatcataa agtaactttg agagataata
    22981 gaagtttcag tgcaatgtgt gctatagatg ctataggaac ctcttgcaca ttcaaccaag
    23041 atataaaaat aaactctatt tgtagtgcta caggtaagga aatagaagta attataaaaa
    23101 atgaaaagat tgagtatgtg aataatccaa acttaagagt gcttcatata aatcttgata
    23161 agcatctaaa ttgggctgct agttgctgaa atataatgaa tttcttctgg acgaagagtg
    23221 attttgatgc ttggacaaac gaagcgggat tatctaatga tgaagatata tattgtttag
    23281 atattaatga ggcaatagta gaatcatata aaatatttaa actcaaacaa aaggtactat
    23341 catagatata catatatgaa aaactaaaaa tgacctttag ataaaaatga taggtaatat
    23401 ccatactata taaattagta tagagtaggg atgtgaaaag tatttaaatc taaaggtata
    23461 tttttagttt tttttagatg attagatagc cttattaagt atatatttat ttataacctt
    23521 tgcaactcca ttttcattat tagtatcagt aacataatct gcaatttcct ttatactatt
    23581 acgtgcattc cccatagcca caccaagacc tgcatacttt atcatatgtt ggtcattttc
    23641 ttcatcgcca acagcaatag cattttctat aggtatattt agattcgtac ataatgtttt
    23701 aagagcagtt cctttattag tagttttacc taaaaattct agataaaaag gagcagacct
    23761 tactatagta tatttattga ataaattttt aggtagttgc tgtatgattt cttccaatct
    23821 ttctggttca tcaactatca taattttaca gattttaata gattcatcaa ttttatcttt
    23881 ttgtatatat ttgacttcta tattatttaa tttcgcttca tgaagtgtat atttgccagg
    23941 cgtttcattt ggtgtaatta aatttgtcat tgtatttatg tgtattttag tcttatattt
    24001 tttacaaacg ttattatata taaaatcaaa atcatctaaa ctcatttcta catcatgtat
    24061 gattttctca cttttaactt cttgaataat tgctccatta aagcatataa cataatcacc
    24121 ttcatctgtt aaatttagtt gatttagaag ttcttttaca ccaggaagag gtctgcctgt
    24181 tgttataact acttttgccc cagccctttt tgcctcctga atggactcaa agacttcagg
    24241 agtaatcctt ttttgtgtat ttaatattgt tccatcaatg tctagtgcga ttaatttgta
    24301 catatcaacc tccaataaat tatatactca aatttcctgt gaaaacgttg acacagctag
    24361 tgttgaacaa agtttcagtg ggaaatatgg tttgttttgt atactattat atcaagagat
    24421 aagtaattta gaaagtttaa taatattaat tttttgataa aaatattact tatgtgagat
    24481 gagaatgtaa aaactttgta atcaataatt ccaaaattct taattacaaa taatggtatc
    24541 gtttgcattg ttaatataaa taaatgtata ttatatagtt agaagtttga ataaatagac
    24601 tgatggtaat ttgttatatt agttagtttt gtgttataaa atattgtaat tccattaatc
    24661 atatgttatt attcaacatg attataatat aaagaatatt tgatatatat aataatttat
    24721 aaaagagtat tacatagtac atgtaaatct atatttataa atacaataaa gtaaagttgt
    24781 aaaggagtgc tttaaatgaa gttttcagag tttaagtatg aaagacccaa ttataatagt
    24841 atgaaaaaag agtttttgag ttgtgtagaa gatataaata attctcgtaa ttataaagag
    24901 caacaaaaaa atatacataa gattaattta ttaagaaata aaatagaaac gttgtcaaat
    24961 atagcttcga taagatatag tacagacaca tttaataaat tctacaaaga agaaaaaaat
    25021 tattgggatg aatatatgcc tttgtatgaa gaattaaatt catattttta taatgccatt
    25081 gtaaattcaa aatttaaata tgatttgata aaagagtttg gagagcaatt tttcacaata
    25141 gtagagtatt ctttaaaaag tttttccaaa gaaataataa gtgaattgca ggaagaaaat
    25201 aaattgtgtt cagagtatac tagactttta gcttcagctg agataatgtt tgatgggaaa
    25261 ataagaaatt tatctggtat ggggaaattc atgtattcta agaatagaaa aacaagagaa
    25321 ttagcaaaca aagcatatta taatttcttt gaagaaaatg aaactaaatt tgatgacata
    25381 tttgataaat tggttaaact tagagataag atgtctaaaa aattaggttt tgaagatttt
    25441 gtagagcttg ggtatgttag aatgatgaga agtgactaca gagaatatat gataaaaaat
    25501 gttagaaaac aagtactaaa atatgttgtt ccaatggcaa atgagcttta tgaaaaacaa
    25561 gctaaaagaa ttggtctaga atatttaagt tacatagatg aaggcgtgga atttttaaca
    25621 ggaaatgcaa gtttaaaggg agattcaaga tacataatta aaaatggtaa aaggatgtat
    25681 tctgaactgt caaaagaaac aaatgaattt tttgatttta tgttagaaaa tgaacttatg
    25741 gatttagaaa cgaagaaagg aaaaggagct ggagggtatt gtacttatat accagattat
    25801 aagtcaccat tcattttttc taattttaat caaactgctg atgatataga tgtattaaca
    25861 catgaggcag gacatgcatt tcagttgtat atgtctagat ggattgatat gccagaaatt
    25921 aattttccaa cgcttgatag ttgtgaaatc cattcaatga gtatggaatt tataacatgg
    25981 ccgtggatgg atttattttt taaagaagat acagataagt acaaattcac tcacttatca
    26041 tcttcaatta aatttatacc ttatggagta attgttgatg aatttcaaca ctatatttac
    26101 aaaaatccta atgtagataa gtcaaaacga aaagaaatct ggagattttt agagaaaaaa
    26161 tacttacctc atagaaaata tggtgataat tcttttttag agagaggttg ttggtggttt
    26221 aaacaaggac atatatttaa aaatccgttt tattatatag attatgtatt agctcaaatt
    26281 tgtgcgttac aattttggaa aaaaatgatt caagatagag atgcaggatg gaaagattat
    26341 ataaatatct gtaaagttgg aggaacaaaa tcatttttag atattgtgag tatgggaaat
    26401 ttgtattctc catttgatga tggctgtatt gagtctatta taggtgatgt taaatcttgg
    26461 tttgatgaaa taaatgacag caaattataa aaaattatga gaattttatt gtataaaagt
    26521 gaaaaattaa aaaatatata aaaagtgatt atttgttgtg aaaatagtct tgtttttaac
    26581 taaattgata aaataaacta aaaaagattt gtacttaaaa aaaaaagaaa atacaagaaa
    26641 tatgcaatat caacatttgg aaaaaaaagt aaaaatgtat acaaaataca tataaaaaaa
    26701 ctattgcaaa ttttatagaa tgtttatata cttaagtcaa cagagataga aaaaagctat
    26761 ttatagaaaa caaaggataa attgcttaat tttaaatttt ctgaaaaaaa taataatccg
    26821 gatgaagagt atgggagact gctttataaa aagcgaccga agggacaagt ttaatcttac
    26881 ttacccaaaa gattagatga aattctcagg taaaggtgac tgtacttgga cggggctctg
    26941 aaaatgattt taacagggaa gacaaattgg gagatttgtt tttcctgttt tttatttatc
    27001 aagtaataaa taaaagtttg aaagagagta aaaaaatgat aataattacg aataatccaa
    27061 aagtaaaaga agaagttcaa ggcagagaag ttttgtttaa agacacaact tatattggaa
    27121 tcttagaagc aagtagagat ttgatacatg aaggttatga acttttatct catccactct
    27181 atgggagtgt aaagccaaat gaaacacctt acaggactgt tatactaaag aaaggaaatc
    27241 gtttggatat aaactcttta accttaattg aagaagcaat catcactgca agtaaatttc
    27301 agaataataa aaagactcca aaatggacag aaagtgttca agatgatttt agagtaattg
    27361 attatgatat attttataat acaatacaaa gaatgcagta tgaataaaaa aaaattgtta
    27421 aaaagttatt aaaacagcct aaatattaaa tctaatttaa tattaaataa aatgagaaat
    27481 ttaattgggg aggataagtc aaaatggaaa atgtgtatga cttagtaata attggttcag
    27541 gaccagcagg gcttgcagca ggtctttatg gagcaagagc aaagttaaaa actctaatcc
    27601 tagaaaaaga caagactggt ggacaaattg taataacaca tgaaattgca aattatccag
    27661 ggtcagttcc aaatgcaaca ggaccaagtt taatagctag aatggttgaa cagtgtaaag
    27721 agtttggagc agaaatgcta agagacaaca ttgttgatac tgagctagat ggagatataa
    27781 aagttttaaa aggggaaaag gcagaatata gagcaaaagc agtaataata ggaacaggag
    27841 caacaccaag aaagattggt tgtcctggag aaaaagaact tactggtaaa ggggtttcat
    27901 attgtgcaac ttgtgatgca gatttctttg aagattttga agtatttgtt gtaggtggag
    27961 gagacagtgc attagaagaa gcaatgtatt taactaaatt tgctagaaaa gtaacaattg
    28021 tacatagaag acaaggattt agatgtgcta agagtgttga agaaaaagca aaagctaatc
    28081 caaagataga gtttttatta gatacagtaa ttgaagaaat caaaggtgat ggaatattag
    28141 aatcagttgt atttaaaaat aaagttactg gagaaactca tgaatacttt gcagatgaag
    28201 aagatggaac aatgggagta tttgtgttcg ttggattaga tgctcaaact gatttattca
    28261 aaggtaaagt tgatatggat gaaaaaggtt acataataac tgatgaagat atgagaacta
    28321 acataccagg cgtatttgca gcaggagatt gtagatctaa gactctaaga caggttgtaa
    28381 ctgcaactaa tgatggagca attgcatcta tagtagctga gaaatacatt gatgaaaaat
    28441 ttgggaacta gaatctgtaa attaagaaat agtgtctgta aatttaatga ataatattta
    28501 aaattaaaat taatatttaa aattaaaata aaaatttcaa gggggaataa gaaaatgtta
    28561 gatttagata aggctacatt tgaagaagaa gttttaaatg cagaaggttt tgtatttgtt
    28621 gatttttgga gtgaaggttg tgagccttgt aaagctttaa tgccagatgt tcataaatta
    28681 gctgagactt atggggataa aataaaattc tgtaagatgg atacaacaaa agctagaaga
    28741 ttagctataa aacaaaaagt attaggtctt ccaactatgg ctatatacaa agatggagaa
    28801 aaagtagacg aagttactaa ggatgatgct acagttccta atatagaaaa catgattaaa
    28861 aaatatcttt aatatgttga taaattatta acattaaaag aaaaaatcta tttttatctg
    28921 aagataagaa gaaaaaggtt tttatgtcca actttgttaa aaaaataatt ttaaataaaa
    28981 taattttttt acaataaata ttttttagta ataaaataaa gatttaaggc ataaaaacac
    29041 aataatatat ctttaaaaaa ataagataaa ttgtaaaatg atataatttt gagaaaaact
    29101 caaatgcata tacacaaatt aaataacagg aggtgcaagt atgcgtcttg aattagggaa
    29161 aatctttata aaagatattc aatttggtga tgtaacagaa gtaaaagatg gagtattatt
    29221 catcaataag caagagatgc tacaagaaat tggtggagat gagcatatta aatctataga
    29281 tatagaatta gctcgtccag gtgaaagcgt aagaataaca cctgtaaaag atgttattga
    29341 gccaagagta aaagttgaag gtaatggggg aatcttccct ggtatcatgt ctaaagttga
    29401 cactgtagga gaaggaaaaa ctcatgcttt aaaaggtgta gctgttgtaa ctactggaaa
    29461 aatagtaggt ttccaagaag gtataataga catgactgga gaaggagcta agtacactcc
    29521 attttctaaa ttaaataatg tagtagtaat agctgaacct attgatggtt taaaacaata
    29581 cgctcatgaa aaagctgtta gaatgatagg attcaaagca gcaatgtact taggagaagt
    29641 agcaagaaat cttactccag atgaagtttc agtatatgaa actaaacctc ttcttgaatc
    29701 aataaaagaa tatccagagc ttccaaaagt aggatatgtt tacatgcttc agactcaagg
    29761 tttattacat gatacttatg tatatggagt agatgcaaaa caaatagtac caactttatt
    29821 atatccaaca gaattaatgg acggagcaat tgtaagtggt aactgtgttt ctgcatgtga
    29881 caaaaaccca agttatgttc atataaacaa tggggttgtt gaagacttat atgcaagaca
    29941 tggtaaagat ataaactttg taggtgtaat tataactaat gaaaatgttt atctagcaga
    30001 taaagaaaga tcttcaaact ggacttctaa attatgtaaa tatttaggat tagatgcagt
    30061 tatagtttca caagaaggtt ttggaaatcc tgatactgac cttataatga actgtaagaa
    30121 gatagaaatg caaggtgtta aaacagttat agttacagat gaatatgctg gacgtgatgg
    30181 aggttctcaa tcacttgcag atgcagatgt aagagctgat gctgttgtaa caggtggaaa
    30241 tgccaaccaa gttgtagttc ttcctaagtt agataaagtt attggacatt tagaagtagt
    30301 agatgtaata gctggtggtt ctgatggaag tttaagagct gatggaacta ttgaagttga
    30361 aatccaagct ataacaggtg caactaatga aacaggattt ggtcatttaa ctgctaaagg
    30421 atattaagaa gtattttatt aaacgattga gtaatgttaa taaaacatat tatagataat
    30481 tataataagt gttatagata tataaaaata tattagaaat ataataagga ggattattat
    30541 gagtttactt agtaataaaa aggttcttat aataggtgac cgtgatggta taccaggacc
    30601 tgcgatagaa gaatgtgtaa aaacagtaga aggagcagag gttgttttct catctacaga
    30661 atgctttgtc tgaacagctg ctggggctat ggacttagaa aatcaaaaca gagttaaaga
    30721 tgctgctgat aaattcggag ctgaaaatgt tgtgatttta ctaggtgctg ctgaagccga
    30781 agctgcaggt cttgcagccg aaacagtaac tgctggagat ccaactttcg ctggaccact
    30841 tgctggagtt gccttaggat taagtgttta ccacgttgtt gaggaaccaa taaaatcatt
    30901 atttgatgaa agtgtatatg aagaccaaat aagtatgatg gaaatggttt tagaagttga
    30961 agaaatagaa gaagaaatgt ctggtataag agaagaattt tgtaaatttt aatgcctaaa
    31021 agcaaaaggg gtgaaactaa gagatgggaa aactaaaagc agtacattat attaatcagt
    31081 tctttgctgg tataggtgga gaagaaaaag ctgatactaa gccacatgtg gcagaaactt
    31141 tacctccaat aagtttacaa cttgataaat tattaggtga agatattgaa atagttggaa
    31201 cagtagtttg tggagatagc tatttcaatg aaaatataga tagtgcaagt gaagaagttt
    31261 tatcaatggt aaaaggtttt gaaccacaac ttttcatagc tggtcctgca tttaatgctg
    31321 gtagatatgg ggttgctgct ggaacaataa ctaaagttgt aaaagatgct ttgaatatac
    31381 cagctttaac aggtatgtat gttgaaaatc caggagcaga tatgttcaaa aaggatgttt
    31441 atgtagtaga aacttctgat tctgcagcag gtatgagaaa ggcacttcct aagatagcta
    31501 aacttgcagt aaaacttgct aatggagaag aaattggaac tcctaaagat gaaggataca
    31561 tagctagagg aataagagtt aactacttcc atgaagacag aggttctaag agagctgttg
    31621 acatgttagt taagaaaata aaaggtgaac catttgaaac tgaataccca atgccaaact
    31681 ttgatagagt tgacccaagt aaagctgtaa aagatttatc aaaatgtaaa atagctttag
    31741 ttacttctgg tggtatagtt ccaaaaggaa atccagatag aatagagtct tcttcagctt
    31801 ctaaatatgg aacttattct atagcaggag ttatggattt aacagaagag acttatgaaa
    31861 ctgcacatgg tggatatgac ccagtgtatg caaatttaga tgcagataga gtattacctg
    31921 ttgatgttct tagagattta gaaaaagaag gtgtaattgg aaaattacat gagactttct
    31981 atacaactgt aggtaatggt acatctgttg caaactctaa aaaatatgct tctgaaattg
    32041 gagcagcatt agtggctgat ggtgtagatg cagtaatatt aacttctaca tgaggtactt
    32101 gtactcgttg cggtgcaacg atggtaaaag aaatagaaaa aactggactt ccagtagttc
    32161 atatgtgtac agtagtacct atttcattaa cagtaggggc taatagaata gttccaacta
    32221 tagcaatacc tcatccactt ggaaatccag cgcttgaccc tacagaagaa aaagccctaa
    32281 gaagaggcct tgtagaaaaa gcattaaatg ctttaactac agaagtagat ggtcaaacag
    32341 tatttgaaaa ataaaaataa cacacacata tattatatag ataaaaatat aaacacacac
    32401 taaaaataaa aaataactat acacaaaaaa ataatataga tttcatgaat ggataaattg
    32461 gagttattca ttcatgaaat cttaaaaaat gccaaaatac ataaacaata atagaatcaa
    32521 atatacaata aaatacaatg attagagctt ttaataagtt taataagatt aaaaaataag
    32581 tgttcataaa atttatggag gtgcacaaaa tgacttatcc agttttaaaa ggggcaggct
    32641 atgtacttat tcatactcca gatatgatag tgcaaaatgg aagtacttgt acagttgaaa
    32701 gagcaacaaa tccagattca gaatttttaa aagaagtaag taatcatata agaagttatg
    32761 aagatgtagt taactacatg ccaaatcaag tttatatagg aaatagaaga ccagaagaat
    32821 taagagattt accaatgcca tggtgtgaac aaaagattga gggaactaga aatggtaagt
    32881 ttggagaaat aatgcctcaa gatgagttca tagctttaat gcaaataagt gatgcatttg
    32941 atttagtaaa attatcacaa gaatttattg atgaagtaaa gccaaaaatc gaaaataact
    33001 accctgaaat tgcaccattt gtaggtaaat taaagggtga tgatatagaa gaaggtaaag
    33061 aattagtagc aacacacata gctgaaggat tataccatga tggaaaattt gttggttatg
    33121 taaaaagagc acatgatgta gatgttaact taaatgccca tacaatgttt gaaaatctag
    33181 tagtaaaagc atctggagtt ttatctgcta ttcaaatgct aagacatagc aaaatagacc
    33241 ctgctgaaat agattatgtt attgagtgtt cagaagaagc ttgtggagat ataaaccaaa
    33301 gaggtggagg aaactttgct aaatcaatag ctgagatagc aggacttcaa aatgcaactg
    33361 gttctgacac aagaggattt tgtgcagcac caactcatgc attaattcaa gcagcagctt
    33421 tagtaaaagc tggcatacat aaaaatgtaa tggtagtagc aggtggagca agtgctaaac
    33481 ttggaatgaa tgctaaagac catgttaaaa aaggtcttcc tgtacttgaa gatgttgttg
    33541 gaggatttgc agtacttgta tctgaaaatg atggtgtaaa tccagttata agaactgatt
    33601 taacaggtaa gcatactgtt ggaactggtt cttcaccaca agcagttatg acagcactta
    33661 taacttcagg tcttgataga gctaatttaa aaataacaga tgttgatgtg tattcagttg
    33721 aaatgcaaaa cccagatata actaagccag caggagcagg agatgttcca gaagcaaact
    33781 acaaaatgat aggggcactt gctgttaaac gtggagactt agaaaagaaa gaattgaaag
    33841 attttgtttc taacaaagga ttaccaggat gggcaccaac tcaaggacat ataccatcag
    33901 gagctcctta tataggattt ttaatagatg atttaactac aggaaataga aatagagcta
    33961 tgatagttgg aaaaggtagt ttattcttag gaagaatgac taacttattt gatggagtat
    34021 cttttatagc tgaaagaaat acaggtgtta ctgaagagac ttcaggaata tctaaggatg
    34081 aaattaagaa gataatagct gaatctatga aaaaactagc attagatatg ttagaagaat
    34141 aggggtgagg atatgtcaaa aaaagtaatc gcagatgtat tcttagaagt agctaatgca
    34201 atagaaagcg gcgaatttgg caaaaaagta aaaattggtg ttactacact tggaagtgaa
    34261 catggtgtag aaaacatggt taatggtgca caattagcaa aatctaattt atttgatatt
    34321 gtgcttattg gaccaaaagt ggaaactgac cttgaagtag ttgaagttaa tgatgaaaaa
    34381 gaaatgcatg caaaaatgga agaattatta gattcaggat atattgatgc ttgtgttact
    34441 atgcattata attttccaat aggtgtatct acagtaggta gagtaataac tccagcaaaa
    34501 ggaaaagaaa tgatacttgc aacaacaaca ggaacaagtg ctactaatag aatagaggca
    34561 atggtaagaa atgccattta tggtattgct acagctaaat caatgggtaa caaatgccca
    34621 aaagttggaa tacttaatgt tgatggagca agacaagttg agaagtgctt aaaagaatta
    34681 aaagataatg gatatgacat ggagtttgcg gattcaatta gagcagatgg tggatgtgta
    34741 atgagaggta atgatttact tgtaggtgca cctgatgtta tggttactga tacattatct
    34801 ggaaatatat ttatgaaagt attttcttca tatactacag gtggagatta tgaagctcaa
    34861 ggctttggat acggacctgg agttggagag gactatgata gaaaggtact tatagtatca
    34921 agagcatcag gttctccggt agtagcaaat gctttaaaat acgcttatga tgttgtaaag
    34981 ggagatataa gtaatgttgc aagaaatgaa tttgcaaagg ttaaaaaagc aaaatttgat
    35041 gacattatat cctctcttac taaaaaagaa gttaaagctg aaaaagtaga agttaaaatg
    35101 ccagataaag aaattgttac tcgtcaaata gctggtgttg atataatgga tttagaagat
    35161 gcagtatctg agttatggaa aaacggaatc tatgctgaaa gtggtatggg ttgtacaggt
    35221 ccaatagttt tagtaaatga agctaaagga gacttagcag ttgaaacttt agttaaagct
    35281 ggatatactg caaaataagt acatttttta tatgtaaata agaacatata gttataattt
    35341 tattgattat aattatatgt tctttttatt ttatatcttg tcaatattta tatattaaca
    35401 caatccattt atttgattct ctagctaatt tattatattt attcaattaa aattaagctt
    35461 tctaactaaa taaaaactaa tgaagtatta aaatgaaatt gataaaatat gaaaaaattt
    35521 taggaataaa gtatctgtta gataaaaata agattaatta gtgatataat ataattaaga
    35581 taaaatttat tatttggagg taaaatcatg agcagagtaa aaaatgtagt agaattgttg
    35641 gaaactaaag gtgttgatgc actatatcta acaaagaaaa ctaatgtaaa ctatataagt
    35701 ggttttcctg atgaagaagc atatgcagtg atttgcaaag atggaaactt tttagttact
    35761 gatagtagat atatggagct tgcagaaaag gtttgcaaag attttgaaat tataaattgg
    35821 cataattttg atagaagtgt ggcaaaggca gttaaaagtg tatgtgataa agtgggtata
    35881 aagaaacttg gatttgaaag aactaatata gtgtttgata aatatgaaga acttaaaaat
    35941 ctgattgaga aggataatgg agaattaata cctacagaaa atattgtaga gactttgaga
    36001 tatgtaaaag acaaagatga aatcaaaaat acaagaaaag cttgtgaaat agcggataag
    36061 gcattagaag aattgattcc acatataaaa gctggagtta gcgaaattga actggctaca
    36121 aggttagagt actttatgaa aatgaacgga gcacaaaata tagggtttga aactatactg
    36181 atatcaggag caaagacttc attgcttcat ggtaagccta gtgataagat tatagaaaaa
    36241 ggtgattttg tacttataga ctatggtgct atgtataatg gctatatatc tgatacaact
    36301 agaactttta tagttggagg agcttctgaa aagcaattag aaatatataa tttagttaag
    36361 gaagctcaaa atgtaggggt tgagaacatg aaagctggtg tacatgcgac aattccagat
    36421 gctgagataa gaaaagttgt caaaaaatat gaagactatt actatcaagg tataggacat
    36481 ggagtcggta gagatgttca tgaagaacct ttcattggta attatggtga taaaatcata
    36541 gaagagggat gtataataac aatggaacca ggtatttatt tccctggatg gggtggagtt
    36601 agaatagaag atactgtttt gattactaaa aatggtccag aaagacttac taaattccct
    36661 aaagatttga tgattttaga caaataattt aatttatata tattttttta cataaaatat
    36721 aacataatct cactattaag ctctatttac atattatagt aaagagggct taattttatt
    36781 gtgagaggtg atagtatata tgtataaaaa acataaattt ataaaaaata taaaaaaagc
    36841 ctggtataaa atcaaattaa ttgatagatg ccttataatt gtaatggcaa tattaatgtt
    36901 tcaatctata tataacttat ttgttaatga agtaaattca caagatacta caactattga
    36961 tgtagtagta agaaccactt cagcagctat atttgggtat tttttaagtg ctaattttat
    37021 aaagagacct ttaaggaagt atacaaatac tgatgtatct tcaaatcttt ttatagataa
    37081 taatttatcc ccaaaaggtc aagagtctaa tcaaaataat ataatgaatg tcaaaaatac
    37141 cattggattt acttcagaag ctgataatta tgaaaagaaa atacctatta ataataatga
    37201 aggttttgaa gaaggagaaa ctagtgaatt gcaaattatt atagctactg taatttgcat
    37261 agtagcatta acagtacttt ttgttgtaag aaattttaca actacaacat cagcttcttt
    37321 aggtactatt tcacagatga gagattttgt ctcaggatgc gtgggttttt tgctaggttg
    37381 tccaagtaaa cctgattaaa aaagtattat caataaaagg tattttaaaa agtatcagta
    37441 taataatatt tagatttaaa tgtatacaat tataattctg taacattcta tattgtattt
    37501 ttatactgat actctaaact atactattta ttaaaatatt ttaacattgt atttaaacaa
    37561 aatattatat ctgtataata taaatttagt ttatatgaat agatgccata tactcaataa
    37621 aaagtttcat actatgagat atgtattgat ttcttctcca aagaacccca atatttgcag
    37681 taattgcacg ttctaatggt attgaacaga gattttctgt attttgaaat atttctttat
    37741 acaatattgt tgaagccaag tcattttcta gtaaataacg tatggtagaa agttgatttg
    37801 agtgtaaaat tatatttggc ttagcttttt caagtctttt tgtaataatc ttatagtgat
    37861 aagaaccttc agataagatt acaaaaggta cattttttaa catataattc gatataatag
    37921 gatgttttgc caattcattt ttgggatttg taactaagta taattctgtt ttatagattg
    37981 tctttgaatc tatatcatca taaatatcac tgtcaattat accaatacaa aaatcaagtg
    38041 tagattcttc aatcatttta gctccaacaa atgttggaac ttcaaaaatt ttaagctcta
    38101 tatctggata aattacatta aacctaggaa gtatttttga aagcaaaaac gtacctaaaa
    38161 ctgtaggaat acctattttg agagaagctt ttctcttttt tcctaaatca agtgcgtcac
    38221 attgaaaatc ctcaaattgt tttataaaat gttttgactt gtctaaaaaa atttttcctt
    38281 caggcgttaa ctctatttta ttacctattc tgtaaaaaag ttcaaagcca agttcttttt
    38341 ctaattcttt aattgctaat gttatggatg gttgagaaat atgaatactt tccgcagcct
    38401 tagtcatgtt tttatattta cagacagttt gaaaatagta taaatagttt atattcattt
    38461 ggtattagtt cctttcattt acatattagt gacaagtata taattaacaa tagcataagt
    38521 tttgtatatt gttgtaaaaa aatagaatag ttttaaacta tcatagcata agtttttgat
    38581 attttcaatc tattaagaat agtgctatta ttaaattaac aaaagagaat agctggtatg
    38641 gtttagagta aatcaatcta tattacagtt atatcaacga atatcaaaaa attatctatt
    38701 aaaaaataaa aattattttt acaattaaat tgttaattaa atcataaaga aaaaagtgat
    38761 attaacaaga taaaattttt ttaatcagaa atgttaattt aataacaaaa tagagtattg
    38821 gggagaaagg agaaatatat tttttaataa tatttgttaa tttaataacg aaaaatgaaa
    38881 gggagtgatt tagtgaagtt tgaattgatg gattttttaa tgaatccttt tgtccttatg
    38941 tttgctgcag taattacagg aattttattt ggaaaaatta aatttgggaa gtttaatttt
    39001 ggggtatcag gagcattatt tacaggatta tttattggat ggcttgcata tagcttgggg
    39061 aatctaataa tagcaaaagg ggaaacagca gcaggatata aggcagcaac tgtaatgatg
    39121 gggaatggaa ttatttcttc tgacttcttt gatttctttt taataatatt tgttgcagca
    39181 gttggtctat tggcagcaaa agatatgaaa gctgtattaa agaagtatgg agcaagattt
    39241 gtaattttag gagttcttat aacttttata ggtggctcta tgacttatgc aatgacttta
    39301 ctatcaagtg ataaaggaag tagtgcttat gaagtttctg gcgtttacac aggagcatta
    39361 acaagttcgc ctggattagc tgctgcttta gaaacagctg gtaaacatgc agaagatgta
    39421 tcaaaagaat ttgaaaaagc ttcaataaaa gataaaaaag aaattttaaa agtagtcgac
    39481 ccagaaggca aattggatgt gaatacaact acatcactga ctcaagagca aatagataag
    39541 tatattgcat atgctgaagc tggtgttggt ataggtcatg cagtcgcata tccttttgga
    39601 gttttaatag taatcttagg tgtaaatttc cttccaaaat tatttaggat ggacctaaaa
    39661 gaagaaagaa gaaaatatga gaaggaaatg aaggaagcaa gagatagtgt ttctggtaaa
    39721 acgataccag aagttccatt taatataata acatttttct tgacatgttt agctggatac
    39781 ttagttggag gaatacatgt atttatggga ccactagggt acttcacact aggtgcaaca
    39841 ggaggttcac taattgtatc tctagttctt ggatatatag gtaagatagg ggtagtaaat
    39901 ttcagaatgg aagaaaaggt tttaaatatt ttaaaacaaa tagggctagt gtttttctta
    39961 gcaatagttg gattaagata tggtggaaaa gtagttgatt caattatgac atctggtatg
    40021 cacctagcct tagtagcaat agcagtaggt gttacagcta tgatgatagg attcttagtt
    40081 ggcaaatacg tatttaaatt aaattggatt ttattatctg gagctgtctg tggtggtatg
    40141 acatcaacac caggtcttgg agctgctgtt gatgctcttg atagtgatga cccagcagca
    40201 ggttatggtg ctacgtatcc atttgcatta ttaactaaag ttatattggt tatagtttta
    40261 cacaaacttc caatgtaaat aagattttat atggggggat atttatgaat aatgaaggag
    40321 catttgaaga attaagtgga agattaagaa ataaagaatt gtgtgattta ataatgaatg
    40381 cagatgaagc tgctaaaatt atagaggatg gcatggttat tggagttagt ggatttacac
    40441 cttcaggcta tccaaaagca gtaccattag cagtttcaga aagagctaaa agtggagaaa
    40501 agataaaatt aaccgtatat tcaggagcat ctttaggtcc agaagttgat ggagcatggt
    40561 cagaagcagg tataattgaa agaagacttc cttatcaaac taattcaata cttagaaata
    40621 atattaataa aggtgttgtt gattatatag atatgcacct aagtcattct actcaatttt
    40681 taaattatgg aactatacct aaagtagatg tagctatagt agaagcactt gcaataactg
    40741 aagaagggaa tattatacca actagtggga taggtaattc tccatctttt ataaaaagtg
    40801 cagataaagt aatagtagag ataaatcttg caaaaccaat ggaaatggaa ggtatggcag
    40861 atatatatat tacggaaaat ccaccaaata gaaaaccaat agaaataaat catcctaagg
    40921 atagaattgg aacaacatat ataccttgtg gtttggataa aattgcagga gtagtaatta
    40981 ccaatatgca agataagacc cgtccattgg gggttgtaga tgaagcttcg aaaaaaatat
    41041 caaataatat tattgctttt ttaagagaag aagtaaagtc aggcagatta tcaaagaatt
    41101 tacttcctct tcaatctggt gtaggaagtg tagcaaatgc agtattatat ggattatgtg
    41161 aatctgaatt tgaaaattta acttgttata cagaggttgt acaggactct atgttggatt
    41221 taataagaat gggtaaagtt acaatggcat ctactacatc tgtaagtcca tctccagaag
    41281 gattaataaa atttgaaaaa gatattgatt tttttaagga taagattata ttaagacctc
    41341 aagaaattag taacaatcca gaaatagcca gaagaattgg tgtaattgca atgaacactg
    41401 caattgaagt tgatatttat ggaaatgtaa attctactca cataatgggg tcaaagatga
    41461 tgaatggaat tggtggttct ggtgattttg ctagaaatgg agcaataact atatttagta
    41521 ctgagtcaat tgcaaaaaat ggtgatatat catcaatagt acctatggta tctcacgtag
    41581 accacactga acatgatgta atggtaattg taacagaaca aggatatgct gatttaagag
    41641 gtcttgcacc tagagaaaga gctataaaaa taatagaaaa ttgtgcccat ccagattata
    41701 aagaacaact tagggattat ttaaatagag cttgtaaaag tggagcaaaa caaacacctc
    41761 acatattgga cgaagcttta tcatggcata gtaaatttat gagtacaggt acaatgaaaa
    41821 aggctgaaac ttttaaaagt gcattataaa atatttaata aattggaggt agattttaaa
    41881 tggaaaaagc agtggaaaac tttgaagatt taagtaagga atacattaat ggttatattg
    41941 agagagcaag aaaagctcaa agagagtttg aatgttatac tcaagaacaa gtagataaaa
    42001 tagtaaaaat agttggaaaa gtagtgtatt ataatgctga atatttagct aaacttgctg
    42061 tagaagagac tggaatggga gtatatgaag ataaggtagc taaaaataaa agcaaagcaa
    42121 aagttatata taacaattta aaagataaaa aatcagttgg tataattgat atagatagag
    42181 aaactggaat aacaaaggta gctaaaccag ttggagtagt agcggcaata actccttgta
    42241 caaatccaat cgttacacct atgagcaatg caatgtttgc acttaaggga agaaatgcaa
    42301 ttattatcac acctcatcat aaagcaattg gatgtagtac aaaaactgta gagatgatta
    42361 atgaagaatt agaaaaaata ggagctccag aaaatttaat acaaatactt gaccaacaat
    42421 caagagaaaa tacaagaaat ttaatttcat cagcagatgt agtaattgcc actggaggaa
    42481 tgggaatggt aaaagctgcg tactcaagtg gaaaaccagc acttggtgta ggggcaggaa
    42541 atgttcaatg tatcatagat agagatgtag acattaaaga agcagtacct aaaatcatag
    42601 caggaagaat ttttgataat ggtatcattt gttcaggtga acagtcagta atagtagcag
    42661 aagaaatgtt tgataaaata atggatgaat ttaaaaataa taaaggtttt atagttagag
    42721 ataaagtaca aaaagaagct ttcagaaatg ctatgtttgt aaataaatct atgaataaag
    42781 atgcagtagg tcaatcagtt catacaattg caaaaattgc aggtgttgag ataccagaag
    42841 atacaaaaat aatagtaata gaagctgatg gaccaggaga agaggatatt attgcaaaag
    42901 aaaaaatgtg tccagttata tctgcataca agtataaaag ttttgaagaa ggtgtagcta
    42961 ttgcaaaagc aaatttaaat gtagaaggaa aaggacatag tgtatctata cattcaaata
    43021 cagttaaaaa tatagaatat gcaggagaaa atatagaagt atcaagattt gttattaatc
    43081 aatgttgtgc tactagtgct ggaggaagtt tctttaatgg tcttgcacct actaatactc
    43141 taggttgtgg ttcttgggga aataacagca tctctgaaaa tcttgattat aagcatctaa
    43201 ttaatatttc tagaattgct tattatatgc cagaaaatga agtccctaca gatgaagaac
    43261 tttggggata aaatttagac aaaaaatatt cattctaaat taaagtagga atatatttta
    43321 atttagaatg aatttaagac ataaattaaa tttaaattag tttattggac taaatttaaa
    43381 atattaagag acatatagaa gaaggaagta ttataattta aaataaataa aaaaatctat
    43441 aaaagagaag gagtggtttt attatggcat taatgacagg agctcaatat attgaaagtt
    43501 tgagaaaatt aaatactaag gtttatatgt ttggtgaaga ggttaagaat tgggttgacc
    43561 atcctatgat tagaccatct ataaattgtg tagcagcaac ttatgactta gctcatgacc
    43621 cagaatatgc agatttaatg acagttactt caaatatcac aggtgaaaaa ataaatagat
    43681 ttggtcatct tcatcaaagt gttgatgatt tgataaaaaa agtaaaaatg caaagactat
    43741 gtggtcaaaa aacagcttct tgtttccaaa gatgtgttgg tatggatgcg tttaatgcag
    43801 tgtattcaac aacatttgag tgtgataaag cccatggaac taattatcat gataattttg
    43861 taaaatactt aacttatata caagaaaacg atttagtagt tgatggagct atgactgacc
    43921 ctaagggaga tagaagtcta tcacctagtg ctcaacctga cccagatatg ttccttcata
    43981 tagtagaaag aagagaagat gggataattg taagaggagc aaaggcacat caaactggtt
    44041 caataaattc acatgagcac cttataatgc ctactataag tatgactgaa gcagataaag
    44101 actacgctgt atcatttgca gttccttcag atgctgaagg agtgtttatg atttatggaa
    44161 gacaatcttg tgacactaga aaattagaag aaggtgcaga tgttgattta ggaaataagg
    44221 agtttggagg tcaagaagct ttagtagtat ttgataatgt atttgttcca aatgatagaa
    44281 ttttcttatg tggagaatgg gatttctctg gtatgttagt agagagattt gcaggatacc
    44341 atagacaaag ttatggtgga tgtaaagtag gagtaggtga tgtaataata ggagcagcag
    44401 ctttagcagc tgattataat ggagcaaaca aagcttcaca tataaaagat aaactaatag
    44461 aaatgactca ccttaatgaa agcttatatt gctgtggtat agcatgttca tcagaaggtc
    44521 acaaaacaga agctggaaac tatcaaatag atttacttct tgcaaatgtg tgtaaacaaa
    44581 atgtaactag atttccttat gaaatagtaa gacttgcaga agatatagct ggagggctta
    44641 tggttactat gccttcagaa aaagacttta aatcagacct taaagttgga acaagtggaa
    44701 tgactattgg cgaagtatgt aacaagtatt ttaaagcttc ttcagtggct tcaacagaag
    44761 aaagaatgag aatattaaga ttcttagaaa atatatgcct tggttcatct gctgttggtt
    44821 atagaactga gtcaatgcat ggggcaggtt caccacaagc tcaaagaata atgatttcaa
    44881 gacaaggaaa cataaatcaa aagaaagaat tagctaagaa aattgcggga ataaaaaaag
    44941 aggaagcttt aaatttataa aaagttgata tataaaggga aattatatat taatgtgtat
    45001 taattaatga aaaaatatgt agtataagag atagttataa aattatggtg gtttttagct
    45061 aagtaaagaa atttttaata aggagaattt acacatgacc tgtggagtta gattttgtgg
    45121 gggctgtaac cctagatacg aaagagggaa ggctcttaca ataatcaaag agcacttcaa
    45181 tggtagggta gattttagta tagcacaaga gaacgtcgag tatgatttgc tacttataat
    45241 tggaggatgc gcaactctat gcgcatccta tcaacaatat aaagtgaaaa atgaatgtgt
    45301 taagatgtgg gattttagcc atatagaaag agttataaat gtgattgaat cagcaatttg
    45361 tgaaacttaa aaaagctaaa tggggggatt tcattgagtt ggcaagaatt gtatcaaagt
    45421 aaattatgta gtgctacaga agctgtaaaa cagattaaaa acggtgatac tgtagtattt
    45481 gcacattgtg taggtgaacc tcctgcacta gtagaggcta tgattgaaaa tgctgaacaa
    45541 tataaagatg ttgagattaa acatatggta agcttgggta gtggtggata tactgctaag
    45601 ggaatggaag ctcattttag agtaaatcca atgtttgtaa gtggaaatgt aagaaaggca
    45661 attgaaaatg gagatggtga ttttacacct gcattcttcc atgaagtacc aaagttgctt
    45721 agagaaaaaa gattaaaatg tgatgttgtt ttagcacagg taactccacc agatgaacat
    45781 gggtattgta gcttaggaac atcagttgat tatacttatg aagccattaa aactgctaga
    45841 acagtaattg ttcaagtgaa tgaccagttt cctagaacct atggtgaggt agtccatgta
    45901 tcagagtttg actatatagt ggaaaaatca caacctttat ttgaactaca acctgcaaag
    45961 attggagaag tagaagaagc aattggtaaa aattgtgctt cattaattga agatggttct
    46021 actttacagc tagggattgg tgggatacca gatgcagtta tgttattttt aactgataaa
    46081 aaggatttag ggattcattc agaaatgatt tcagatggga ctttggccct ttatgaaaaa
    46141 ggtgttataa atggtaaata taaaaatttt gataaagaaa aaatgacagt taccttctta
    46201 atgggtacta aaaaattata tgactttgca aataataacc ctgcagtaga ggtaaaacca
    46261 gtagactatg tgaatcatcc agcaattata atgaaacaac ataagatggt ttctataaat
    46321 tctgctatac aagttgattt aatgggacag gtagttgcag aggctatggg acttagacaa
    46381 tttagtggtg ttggaggtca agttgacttt atacgtggag tgtctatggg tgaagatgga
    46441 aaggcaatta tagctatgcc ttcaataact acgaaaaaag atggtacagt aatttcaaaa
    46501 atcgtgtcta ttgtggatga aggtgcacct ataacgacat caagaaatga tgttgactat
    46561 attgtaacag aatacggtat agcagaatta aaaggaaaat ctcttagaga aagagcgaga
    46621 aatttaataa atatagctca tccaagtgta agagaaagtc tagcagtaga atttgaaaag
    46681 agatttaagg agaaatatta ataatagagg ggtgttaata atggaagcac ttagagtagt
    46741 tccaaagata ttttactttg atacatttaa agaatttaat gaagaattta aaataggaaa
    46801 aaatgattta gtaattacta atgagtttat atatgagcca tatatgaaac ctcttggaat
    46861 agatacaaat ttaatttttc aagaaaagtt tggaacaggt gaaccatctg atgaaatgat
    46921 agactccatg acaaaggaaa tgaagaaata caattttgat agaattattg catttggagg
    46981 aggaacaata gttgatatat gtaagatatt agcacttgat gttccagaaa agtctattga
    47041 tttatttgaa ggagaagtat cacctaaaaa agtaaaagag ttagtagtag ttcctacaac
    47101 atgtggtaca ggtagtgaag taacaaatgt agctattgca gagttaaaat ctaaacatac
    47161 taaaaagggg cttgcagtag aagaaacata tgcagactat gcagtgttaa ttccagagac
    47221 agtaaaaggg ttaccttaca aattctttgt tacaagttcg gtagatgctc ttatacatgc
    47281 tattgagtct tatctttctc caaaagcaag tccatttaca gagatgtatt ctctacaagc
    47341 aataaaaatg attatggatg ggtataagaa gatagttgat aaaggagaag aagagagatt
    47401 taatcatctt agagactttg tacttgcatc aaattatgca ggaattgctt ttggaaatgc
    47461 tggttgtgca gcagttcatg cactatctta ttcaataggt ggagctttcc atgttgcaca
    47521 tggtgaagca aattatcagt tctttactga agtatttaag atgtattcta gaaaaaaacc
    47581 taatggaaaa attataaaat gtacaaaaat tttagcagat gcccttgaat gtgaccctaa
    47641 ctgtgatgtt tatggagaat tagaaaagtt tttaaataaa ttaatagcta aaaaagcttt
    47701 gagagaatat ggaatggtgg aaagtcagat agatgaattt acagatagta caatagcaaa
    47761 tcaacaacga ctacttgcta ataattatgt agagcttagt agagaggaaa taagagagat
    47821 atttgcaaat ctttattaat acaatttttt atttatatgt atagtcaaca taaacaatgc
    47881 taattatatt tatgtacaaa taaaaataag ggaacttaaa ttttaattaa agttaagttc
    47941 tcttattttt atatggagtt aaatataagt atatttttaa tttaaaatat gcttatattt
    48001 aagtctatta ctttatttga aacaatttgt agaatttcaa attccttgac agtaataagt
    48061 aagtaattgt ataatcaaac tgcaatatat taataatatt agtgaaaatg aaaggattat
    48121 aaaatatgta taaatcagaa aatttttcaa aaaaatctgt attaataaat tcatttttaa
    48181 gtaaattttt aaaaataatt ggatggggtt tggtaagctt atttacatta tcagccttag
    48241 tactttcaac tatttccatt ataattggat gggctgtaat acttcctatt caagtatgtg
    48301 cagtattttt acttatagga ggagtttgta tttttaaagg taagagatta ggagaccaaa
    48361 tttctcgtta taaaaaatat tgtacaatta taaacaatag aaatattatt ccagtagaac
    48421 ttattgcaga aaacacatct aaatcattaa attttataat aaaagatgta caaaagatga
    48481 tagacaaaaa ctactttata aatacttata ttgataaaag aaataatcag attgtgttaa
    48541 caaatgaaga ttttgttcca ccaagatatg aagatgtagc atatgaagtc aaagaagaag
    48601 aaaagactga tgaatccaat gaagttgata caattataaa taaaggaatg aattatctaa
    48661 cacaaataaa agaagctaat aaaaatatta aatcagaatc aatgggcgat aaaattgtac
    48721 aagtagaaga tgttacaagt aaaatatttg atgttgtaaa acatgaccca tcaaaactta
    48781 ctcaaataca aaaatttatg gattattatc ttccaaccac tttaaaacta ttaaattcat
    48841 atcatacatt ggaagaacaa ggaattgata gagaaaatat aacaacaact atggaaagta
    48901 ttgaaaatac aatgaatacg attgtagtag cttttgaaaa tcaattggac tatctgtttg
    48961 aagatgaagc cattgatatt tctacagata ttactgtttt agagaatatg ttagtccaag
    49021 aaggattaac gagtggtaaa ttttaacttc aatagaaaaa atattctgtt atttttaaac
    49081 aattaattag tcataaagta taaaatgtgt attatataag ttaatcagta ctttatgaag
    49141 gaagaggaga gaaagataca atgaataatt tagatgatat accagttatg ccaactttga
    49201 cacttgaccc atttggagaa gaatcaaaca ctaatgatat agataattct gacttattaa
    49261 tgaaaaagga tgaaaaagac ccagaagaag aaaaactgtc tgaatctgaa cgcaagatgg
    49321 taaaggaatt tgcggaaaaa atagatatta caaatacaaa tatgattttg caatatggag
    49381 ctggtgctca aaaaaaggta gcaagttttt ctgaaacagc tttaaagagt gtaaagacaa
    49441 gagatttagg agaagttgga gatatgctta caaatctagt tactgattta aaagcatttt
    49501 cagcagatga aaaagaacaa agtggttttt taggaatttt taaaaaggca aataataaaa
    49561 tttcaaattt aaaaactaag tatgatagtg cagaggtaaa tgtggataaa gtaagcaaag
    49621 agcttcaaaa gcatcaggtt aaaatattga aagatatagc aatgcttgat aagatgtatg
    49681 aattgaactt agcttactca aaagagctta ctatgtatat tatagcagga aaacaaaaat
    49741 taaaggatat gaaagaatat gagatgccaa agttgagaga aaaagctcgt ttatctggtt
    49801 caacagaaga tgctcaatct gtaaatgata tggtttcact ttgtgacaga tttgaaaaga
    49861 agattcatga cttagaatta actagaatgg tatcgcttca aatggctcct cagataagat
    49921 tggttcaaaa tagtaataat ctgatggcag aaaagattca atcaactata gtaaatacca
    49981 ttccattatg gaaaaatcaa atagtgcttg ctcttggaat ttctcactta aatcaagcta
    50041 tgaaagcaca gcgtgaagtt agtaatatga caaatgagct tttattaaag aatgcagaaa
    50101 ctttgaagat gggaacaata gaaacagcaa aagaatcaga aagaggaatt gtagatatag
    50161 acacaattaa gaaaactaat caatctctaa tatctactat tgatgaggta gttaagatac
    50221 aacatgatgg tagaataaag agacaagaag cagaagttga attatcaaaa atagaaaatg
    50281 aacttaaaag caagcttctt gaatttacag ttaaataaaa attatactaa gataaaaaga
    50341 gactgtctca aaattggata tgtccctgtc aagtagacag acttaaaaaa ggctatcttt
    50401 actcggaatg agttcgatat tcaatcggac tcattccgtt ttttaattgt attctttcat
    50461 tattataaaa ataaatataa tcatttatta gactaatcat ttcctttgga tctgtagtat
    50521 caattaaata tattaattca gatttaaaat gactgaaaaa actttctata ggagcattat
    50581 cccagcagtt gccccttcgg gacattgatt gtataatacc attatcctta agccttttag
    50641 agtaagatct acttgtatat tgtgaacctt gatctgtatg taaaatacaa tctttagcta
    50701 aattcatatt tattagttga tttactgtat tattaactaa tttagtatcc atttttgtac
    50761 ttatctcata tgccactatt tcaccattaa ataaatcttt ggctacattc atatataaga
    50821 attttttacc gataggtatg tatgttatat ccatacaaat tttttctaat ggttgattag
    50881 ctctaaattc acgatttaat atattatcat atatctcata tgatttaaac ttacgtctat
    50941 atactttctt tctaataata gatttaatac ctagttcttt catcaaccta tatactctct
    51001 tatgattaat aggggtatca agtattttat ttaaataagt acaaattctt tttctaccat
    51061 aagttcctct atatttttta tgtatttcaa ttatataatc cttaataatt ctatctttga
    51121 tacccctatc ggaagatttt ttagaataac ttagccattt atagtaaccg cttcttgaca
    51181 cacttatcaa cttacacata ctagaaatac tatacttcct agacagaagt ttaattgttt
    51241 taaatttatc gcttactttg acttgtccaa tagggtactt aactttttta agaattcatt
    51301 ctccatgcgt aagtacttca attcttcttc aacagattta aattttctat tatcattttc
    51361 tagactaata tttttaaatc tgcccctagt ttcatcatca aatgcagttt tgcctttatt
    51421 tttataatct tttacccagt tttgaacttg tgttttgctt cttatattca gcatctcagc
    51481 aattttgtat gatgagtatc cttcttttat atataagttt acagctttta gcttaagttc
    51541 tttgctatat tttttgtgtt ttttagacat agaaaaaccc ccttagagta tacgcttttt
    51601 agacctatta taatagcctt ttatttaaat gtctactata aggggattat actaaaatag
    51661 agataagtct ctttttcatt gtttaaatga ttaaagtata tttatattat aaatttctat
    51721 tatcttttaa cataacatca tgagctccac cttcaactat actagtagaa gatacaagtg
    51781 taagtttagc atttttttga agttcaggaa tgctaagtgc tccacagtta cacatagtag
    51841 aacgaacttt acttaaagaa agagttacat tgtctttcaa agaaccagca taaggaacat
    51901 aagagtcaac accttcttca aaagaaagtt tcttatctcc accaaggtca tatctttgcc
    51961 agtttctagc acgagcagaa ccttctcccc aatactcttt catataacta ccatttatat
    52021 taactttgtt agttggactc tcatcaaaac gagagaagta tctaccaagc attataaaat
    52081 ctgcacccat agctaaagct aaagttatat gatggtcata aacgatacca ccatcagaac
    52141 aaattggaat ataaatacct gttttttcaa agtattcgtc cctagcttga gcaacttcaa
    52201 ttatagaagt agcttgtcca cgtcctatac ctttttgttc acgagttata catatagaac
    52261 caccaccaat accaacttta acgaagtcag caccagcttc agctaggtaa aggaatccct
    52321 cacggtcaac tacatttcca gctcctacct taactgtatc accatatttt tcacgaatga
    52381 aatcaagtgt tattttttgc cactcagaaa aaccttctga agaatctata cataaaacat
    52441 cagcaccagc ttcaactaaa gcaggaacac gttcagcaaa gtctctggta ttaataccag
    52501 ctccaactac atatctctta gaagaatcta aaagttcaag tggattttct ttatgagaag
    52561 agtaatcttt acggaatacc atatacataa gattaccatt gtcatcaagt acaggaagag
    52621 aattaagttt gtggtcccat ataatgttgt ttgcttcctt taaagtaaca tctttgttag
    52681 cataaactat agatgacata ggagtcataa attcactaac tttagtatct aaatccatac
    52741 gacttatacg ataatcacga cttgcaacaa tacctaataa tttaccagtt gaagttccat
    52801 cttcagtaac agctactgta gaatgtccag ttttttcttt taagtcaagt atgtctttta
    52861 atgtgttatc aggtttaata tttgaatcac ttacaacaaa accagctttg tgggatttaa
    52921 cacgtgctac catagcagct tcatcttcaa tagtttgaga accgtatata aatgaaatac
    52981 caccttcttt agcaagtgca atagccattt tgtcatcaga aacagattgc ataatagcag
    53041 aagtcaaagg tatattcata taaatatctg cttcctcgcc ctttttaaat ttagtaacag
    53101 gtgtttttaa acttacatta gcaggtatac aatcagtaga agagaaacct ggtacaagaa
    53161 ggtattcact gaaagtatgt gatggtgtat caaaataaaa cgccattttt taatcactcc
    53221 ttatattttt atacttttta taattgatag tattaattat aacttattct ttaattttat
    53281 taaagtattt tgtcaaatat tgagattttt gaaaaattta aactatttta attgaatttg
    53341 tttaatatat attgccttat agggtaaagt tgtatattaa ataatgttta gtgtttaata
    53401 gtagctaatt tgaaattaat ctttagttat tcatataatt acatttgtat agtttatatt
    53461 gataatattg ctagagagtg cataaacctc aaaaatacag ataaattaaa tctatattag
    53521 tatatccaaa agtacattta ataaatttat ttaactgaat tattattttc atttctatta
    53581 aatttaaata ttttataaaa atagtattat ttacaaaatc atatactcca aaatactaca
    53641 taaataaaat taaacaaaaa ctaaaatgac aaatgtcatt atctaaagtg acatagaaac
    53701 ggtttcttaa agttgttact ataaaatata aactctataa taaaagtata acaaaaggga
    53761 atcatatcga tatagaatat aattcaagag gaggtaaaat tatgagtagt tctaatcaat
    53821 cgacaataaa gacatctata cagcgagtag ggaaattttt aagtggtatg gtaatgccaa
    53881 atataggagc ttttatagca tggggtctta taacagcatt atttatacca acaggatgga
    53941 tgcctaatga aaaattgagt actataggcg acccaatgat aaaatacctt ttaccactat
    54001 tgatagctta tactggtggt aaagcaatag caggacaaag aggtggcgta ataggtgcag
    54061 cagcagctat gggagtaatt gttggggcag atataccaat gtttatcgga gcaatgataa
    54121 tgggaccatt tgcaggatgg gtaattaaga agttcgataa atttgtagat ggaaagatac
    54181 caacaggatt tgaaatgtta gtaaataact tttcaatagg tataataggt atgcttcttg
    54241 caatactagg attttatgca attggaccag caatagtagc agggacagct cttatagaat
    54301 ctggagttca atttatagtt agcaaaagtt tattgccttt agtttcagta tttatagaac
    54361 ctggaaaagt attattctta aacaatgcaa taaatcatgg tatattaggg cctataggta
    54421 tagctgaggc aaaagaagca ggtaaatcta taatgttctt actagaaagt aaccctggac
    54481 caggtttagg agttctatta gcatattgga tgtttagtag aggaagtgta aaacaatcag
    54541 caccaggagc tgtaataata catttctttg gtggtatcca tgaaatttac ttcccatata
    54601 ttttaatgaa tccagtttta atattggcta caatggcagg tggagcagca ggaatattga
    54661 ctttttcaat attaggagct gggcttgtag cagcaccatc accaggaagt atatttgcac
    54721 ttatggcact agctccaaag ggaggattac ttccagtatt agcaggggtt gcagtggcaa
    54781 cagttgtatc attcttagtg gcagcaccat ttgttaagag agcttcagca aatgaaagtg
    54841 aagaagacag tacttcttta gaagaagcta aagcaaaaat gagtgatatg aaatctgcaa
    54901 gtaagaactc tgagaaaaat attgagaaaa aacagcttga agtaaatgag attaagaaga
    54961 ttgtatttgc atgtgatgca ggaatgggtt cgagtgcaat gggagcttct agatttaaaa
    55021 atagaataaa aaacttagat ttagatatag aaattacaaa ttcatctgtt gataatcttc
    55081 cagatgatac acaaatagta gtaactcaca atacattagt tgaaagagtt gctaagaaca
    55141 attcaagtgt tgagatagtt tcaataaaca attttttaaa tgacccaaat ttagatacat
    55201 tatttaaaag attagaatct aaataaaaat aaaaagtacc actaattagg agtggataat
    55261 ttgaaaaaaa agaagattac atctaggcaa aaaaagataa tactaatgat agtagaaaat
    55321 tcaaagaaaa acattccaat aactatttct gagattgcag ggacgttaga gttaagttca
    55381 agaactgtac ttagagatat gtctggaatt gaaaaatggt ttgatgaaaa tgattttaac
    55441 tttgtaaaaa aaccaggagt aggattgatt ttagaggaaa atatagaaaa tcaaaacttt
    55501 ataattgagt tgttagaaga agaaaaaata gaaaaagaat acagcaagga agaaagaaat
    55561 ttaattatat tatcaaagct actagtttct aacgagccag taaaatctta ttattttgca
    55621 aaaatattaa aggtatctga aggggtactg aataatgatt tttcattagc aagtaaatgg
    55681 ttggaaagat ttgatattga gcttgttaga aaaccagggc ttggtgtcta cctaaaaggt
    55741 caagaaaaaa gctttagaga ggcatatgta aatcttatat acgattcatt caatgaaaaa
    55801 gaaatactag atatggtcag aaatataagt gaaaatatac aaacagataa agcaatagaa
    55861 attttaagtg aaaatagact tttaaattta atggataggt gcataataat aaaagttgaa
    55921 gctacattaa caaagaagct ttcagattta gatgtaaatt tagctgatag tgcatatata
    55981 gggcttgtag ttcatatttc attagcctta cagaggataa aaaatggtga aaatataact
    56041 atggataaag agtttctaaa agaattatct ataacagaag agtttaaatt agctaaagaa
    56101 atcgtaaaag gtatggaaac agattttaac atggatatac cagtagatga ggttggatat
    56161 ataactatgc atataagagg tgcaaagcaa aggtcatctt caaatcataa ggctttaaac
    56221 ttggatgata tagaaataat ggaaattaca aataagatga tagatttggc tgaagatgaa
    56281 tttaaaataa gtttaaaaaa tgacgagagg ttatttaaag acctagcaaa tcacttagga
    56341 ccttctataa atagattgaa tatgggatta gaaataagaa atcctctttt agatgaaata
    56401 aaaagcaagt attcatatgc ctatgatgga gttgaaaaaa tttctagaat tataaaagat
    56461 aaactaaata taaattcaat acctgaatca gaaataggat atattgcaat gcactttgca
    56521 tcagccatag aaaaaaatct tatgatgaat actaatataa atattgttgt ggcatgtccc
    56581 actggaatag gtacttcaag attcttatca actaagattg aaaataaatt tcctaattta
    56641 aatatacttg aaaccatatc agctataaac atagatgaag agtatttaaa gggaaaagat
    56701 gttgatttaa tagtgtctac agttgagtta aatacaagtt taaattatat atgtgtaggt
    56761 ccatttatga gtttagatga tgaacagata atcaaggaaa aaataaaatc tatagctcaa
    56821 aataaattaa ttaatttaaa tacaaaaaat gatactaaaa gtaagaataa agtatatgaa
    56881 cagataacag aaagtatgaa tataggaaaa gatattcttc aatttttaga ggaaattaga
    56941 tttgaaaaat ttgaaagtaa agatttaagt gagcttattg aagactcatc aaaaatattt
    57001 gcaaaatcta gtgaagatat aatgtctata aaagaatcgt taaaagaaag attaaaaatc
    57061 tcaattccat atatagaaga atcaaaaatt ctattattac attgtatgag tgaaagaata
    57121 gatattatga agttatcaat aataaaactg gagaatcgaa tcgtgctaga ttcaaatgaa
    57181 gaaatcgaca atgtagtttt tatgttactt cctaaaaatt caccaagtta tcaaagacaa
    57241 ataatgagtg aaataagtgg ttcattaata gacaatataa tttttactaa taagataaat
    57301 aaattctcta tagaagagat gacattagag gtaaaagata tagtttttaa tttttataca
    57361 aacagattaa aagcatttat agatgaataa aattaatatt aaaaaggaag gttgatacta
    57421 tgagtaaagt attgaatgag aataatatat ttttagggtt agatagtgtt tcaaaggaag
    57481 aagctataac tttagcagga agaaaattag ttgaaaatgg atatgtaaaa gaagaatata
    57541 taccagcaat gctagaaaga gaaaaagtaa tgactacata tatgggaatg ggagttgcta
    57601 ttccacatgg tgtaaatgaa gcaaaaaaag aaatattatc ttctggaata gttatactac
    57661 aattccctaa tggtatagat tttgatggag aaaaagcata cttattaata ggaatagctg
    57721 gtgttggaga tgaacattta gaaatactat ctaatatagc agtagtttta gatgatgact
    57781 taacagaaag actaaaaaat tcaaatgaca aacaagcttt tatggaagct tttgccaaat
    57841 aatcttagga gggatttaaa atgaaaaagg caatccagtt tggagcagga aatattggaa
    57901 gaggttttat tggaggcttg ttagttaagt cgggttatca tgtagtattt gctgatgtaa
    57961 atgaggagat attaaactca ataaataaag ataaaaagta tactatacac ataagagatg
    58021 tagaatgtat agatgaagta attgataata taagtgcggt ttcatctata aaagaagaaa
    58081 taatagatga gattgtacag gctgaaataa taactacagc agttggacct ttagttttga
    58141 ctaaaatagc ctctacaatt gcaaaaggta taaaggctag aaaagaaaag ggtttaacaa
    58201 gtaatctaaa tataatagcc tgtgaaaatg caatatacgc aagttcttct ttaaaagaag
    58261 aggtacttaa gtatttaaat aaggaagaag tagaatattt agagatatat gttggttttc
    58321 caaactgttc agttgataga atagttcctc ctggtaaaaa tgaaaatcct cttgatgtaa
    58381 cagttgaaaa tttttatgag tggaatgttg aaaaacaagg atttaaaggg gaaataccaa
    58441 ctatagttgg aatgaattta gctgacaacc ttatggctta tatagagcgt aaattgttta
    58501 cactgaatac aggtcatgct ataacagctt atataggata tttaaaagga tataagacta
    58561 tagaagagag tataaaagat aaatttatct gtgatatagt caaaagtgca atggtagaaa
    58621 gtggagaagg attgattaaa aaatacaatt ttgactcaga agcgcactat aaatatattg
    58681 ataaaatttt aaatagattt aaaaatccat atcttaatga tgatgtatta agagttggaa
    58741 gggaaccatt aagaaaacta agtgataaag atagattgat taaaccttta atgacagcaa
    58801 aatcatatgg attatcagtt gacaatttga ttcttggaat aggtgcagcg cttcattata
    58861 ataatagtga agatactcaa agtgtagaat tacaagagtt gataaagtct aatggtgtaa
    58921 aaaaagctgt tgctaaaata gctaatataa gcaatgacga agaactttta aataatatcg
    58981 aaaaaagtta tatatttatg aaaaatttat aaatttactt gaaaaaaagt agttctgata
    59041 aagtaactat aatatattat tctacacagt ttgaattatt tgttgaataa tagagaatta
    59101 taatatataa ttaaaaggaa taaattaata tatttacctg tatatgtttg ataatatggt
    59161 tcaaatgttt ctactaagct accattaata gcttgactac aggagtgaag aagatatagt
    59221 ttatacttat tttaaattat atattaagtt aattactttg aattttaagc tcctgtctat
    59281 gatatagaca ggagcttttt tagtatttta atataaaata cttacaagaa tagatagcaa
    59341 aattagaaca aatataattt ttttattaat ataactttag aaagggctag gtggaagttc
    59401 aatgaaggct ttacaagaaa agatattgag agaaggcagt gtttcaggaa atgatatact
    59461 aaaagttgat agttttttaa accatcaaat tgatgtagct tttttaaatg aaataggaag
    59521 agaatttaaa gagagattta aagaagaaaa ggtggataag atatttacaa tagaggcttc
    59581 aggaatagct atagcatcaa ttgtttcaca gtattttgat aatgcacctg tagtatttgc
    59641 aaaaaaatca gaatctaaaa atttggataa agatgtatat gagactaatg tatattcatt
    59701 tactaaggca agagaatatt cagttaaagt ttctaaaaag tacataaaca aaggtgaaaa
    59761 tatattgata gtggatgatt ttttggcaaa tggaagagct gctcttggac ttaaagattt
    59821 aattgagcaa gcagaagcta atttagttgg agtaggaatt gtcattgaaa aaggatttca
    59881 agctggagga gcacttttaa aggctaatga tgtaagatta gagtctttgg cggtagttga
    59941 atctatagat aatggaactg taaaatttag gtaatttatg atattatata cttgtgttta
    60001 gcacatattt taactatgta attatggagg attttttatg atatttttta tagacacagc
    60061 caatattgaa gaaataaaag aagcaaatga tttaggtgta atatgtggtg taactactaa
    60121 tccatctcta atagctaaag aaggtagaga ttttatagag gtagttaaag aaataagtga
    60181 aatagtagat ggaccaataa gtgcagaagt aataagttta gagcatgaag gtatgataga
    60241 ggaagcagaa aaattatcta agatacataa aaatatagtt ataaagatac ctatgacagc
    60301 agaaggtcta aaagcggtga aagtactatc aagtaaaggt ataaaaacta atgtaacact
    60361 tatattctct gctggacaag ctcttttagc tgctagagct ggagctacat atgtaagtcc
    60421 ttttgttggt agattggatg atatatcaca aaatggtctt gatttaattg aagaaatcgt
    60481 tgatatattt agtgtaaata gtattgaggc tcaaataata gttgctagtg ttagaaatcc
    60541 aatacatgtt ttacaagctg ctagaatggg agcagatata gcaacagttc ctcttaaagt
    60601 tataaaccaa atgattaagc atcctttgac agacaaagga atagatagct ttatgaagga
    60661 ttgggaaggt gcttctttaa aattataatt ataaactacg ctttataagt atattaaaga
    60721 ggttattatg gaaattctaa gtaataaggc taggctattt tgcctagtct tattttggtg
    60781 tataatatat tgaaacatat tattgaaata gattaatttt ttaagtgagg aggcgaaaaa
    60841 tggataaaat attaagagag atacaaagag aagataaaaa aaatccatat actgaccaag
    60901 aacttgctga aatgttaaat gtagctaggt ctgaagtgat atcagtgagg aaaaaaaata
    60961 atattttaga ttcgagagaa agaagaaaaa aaatattaat aaaagatata tcaaaaattg
    61021 taagtgaaaa tcctcaaatg tctgaaagaa aaataactga gatattaata gagaatggat
    61081 ataatatatc gagaagtgca gtttcaaaat tattaaaaga agaaaattta aatcagtcaa
    61141 agattgggat taaagataaa gagccagcat catcaggaaa gtatgtgaag gtgagtaaag
    61201 ttgtagaaga agatggattt gaagaattga taggtataaa gggaagtttg aaagaaaagg
    61261 taaatctagc taagtcagca ataatgtatc ctcctaatgg ccttcatact ataatatatg
    61321 gagaaacagg ggtaggtaaa agtgaattag ctacatgtat gtataaatat gctataaaaa
    61381 acaacatcaa agaagagaac agcccattta tagtgtttaa ctgtgcagac tatgcagaaa
    61441 atcctaatct attgatagct caactctttg gggtagtaaa aggtgcatat acaggagctg
    61501 atgcaaatag agaaggtctt gtagaacaag ctaataatgg aatattgttt ttagatgaaa
    61561 ttcatagatt gcctgctgct gggcaagaaa tcttattttc attaatagat agaggagaat
    61621 ttagaaggtt aggagaaagt agttctaata gaaaggcaaa tgttctcata ataagtgcta
    61681 caacagaaaa cccagattct aatttacttc aaacatttag aagacgtata ccaatggtaa
    61741 taaatttacc taatatgagt gaaagaccaa aatctgaaag atatgaaata ataataaagt
    61801 tttttgaaag agaagcaaag cgagtaaaca agaattttat aataactaaa gaagttatgt
    61861 gtgcacttat gaattataag tgtactggaa atataggtca gttaaagagt gatatacaag
    61921 taacttgtgc tagagctttt agtaaatcaa tattttctag cgataaggta attatagatt
    61981 tagatagttt aagggattat ataaaaagtg gatattatga ttgtaattgt aaaaatgaaa
    62041 agtattctga gttttcagtt gaggatatat atatagatat cagtgaattg gtgagtggaa
    62101 aaaatagaat tttatctgat aatgagataa gcgagatata taattatgca gaaaaagagc
    62161 ttaaggtttt ggaaagtaag tgtttttcag aagaagagtt aaaaaatgca tttatagaaa
    62221 agttagataa gaagtttaat gctattaaaa acaataaaaa tttatctgat agaagaagtc
    62281 gaatagactg gggaggacaa ttaaaagagt ctacattgga gataatggat aaggtaattg
    62341 tatttattaa ggagcagttt aagcatgtaa atcaaggatt gtacttggct ctagcaattc
    62401 acattgagca tgctataggt aggataaaag atggaaaaac tataataaat ccgagtttag
    62461 ataaaataaa agcatcaatg ccaatggaat atgaattgtc aagatatata atgggtatag
    62521 tggaagattt gacacaagta acatttccag aagatgaact tggatatttg gcttattata
    62581 taaataaatt ttgttataat gaggaaagta taaaagataa agttaaagta gtaatagtta
    62641 cgcatggtaa agttggaatt gaaatgtcta aagttgtaaa tcatatttta ggtatagaat
    62701 gtacactagg tatagaaata gcacttacag attcaccatc agaggggata gagcatgtat
    62761 tagaagagct tcaaaagata gaagctagaa aaggtattct agttctaatt gatatgggtt
    62821 ctttggtaat tcttggtgat gaggttgaaa aaagacttgg aattagatgt aaaactgtaa
    62881 acagagtaga tacgttattg gcaatggaag caggaaaact tgcaaccatt gaaggaaaaa
    62941 gtttagatgg tataatagca gatttaaaga aaaataagaa ttatgccatg ataaatacaa
    63001 ataaatttag ttacagaaaa aacgagtatg gaaagaaaaa tgtaatcatt actttatgtt
    63061 tgagtggtgt aggtacagct ttaaatttaa aagagcatat agaaaaacaa attaaggaat
    63121 atgacacttt aattgaggta aagccagttg cttttttaaa taataattta gaagaagagt
    63181 taaatgatat tgaagctgaa tataatatta ttgctatctg tggaactatc gatatagatt
    63241 ataaaaacgt accttttatt tcttataatg aggtacttgg taaaaatggt attaataaag
    63301 tattagagca tttaaacagt aaacaaccag ttttaaatat tgaaaaccag ttgaataatt
    63361 taatacatga agatttgatt ttatacaatt ttgaaggaat ttcaaaggag tatataattg
    63421 atactcttgt ttcaaagctt gaagaaggtg gatatgttga ctcaaagtat atattaagtg
    63481 tatataaaag agaagcaatg ggaagtgttg taatggcctc taaggtagcc gtgccacatg
    63541 gacttccaga aaacgttata aagcctgcaa tagcaatcgc aaggttgaat aagcctatag
    63601 tgtgggacaa taaatttatg gtggatttgg tagtactact ggcacttaaa gaaaataata
    63661 agaaagaaat acgaagctta ttttcaaaga ttaatgacca acatacttta gaagttttat
    63721 taaatactga agataaaaat gaaataaaag aacttttaag ctaattatta gtacagagaa
    63781 gaaagacaaa aaatttgctt tagtataaaa tttaaaacta cttaattgct aagtagttaa
    63841 ttactcgata taaggctagt tctatgaaat ttatttatat gattttatag aattagcttt
    63901 tttcttggta aataaaatat atgaatttat ttatgcaaaa agtaaatttt attggacaat
    63961 tctatgagaa tttttcctat atttcttata aaatatagaa tatataaaaa taaattaaat
    64021 tcataataaa taattttata aaatagttgt taaacataaa aaaccatttg taaaaaacaa
    64081 aaaaatcatt tctctaacaa aaaaaaccac aaaaaatata aaaaagtatg gctagttaag
    64141 tgattaacaa gtaaaaatta aaataaaaat caaaaaaaat ttatcaatga aagtcagcta
    64201 aaatcctgga agagagcatt atttttggga aaacattttc ttttgtttca caaaaaaagc
    64261 cactttttgg cacacaacat gctttatata aagtcaagaa aaactaaggt ataagacaaa
    64321 ggcaattagg aggtgtaaat atggagttta taaattttgt aagtgataaa gttatagacc
    64381 ttaaaatgga ttgtaaaacg aaagaagatt tctttaaaga aatacataat aaagtttttg
    64441 atttaggctt tgtaaaagaa gaatttggag aaaaaatatt agcaagagaa aatgtatttc
    64501 caacaggttt aaatttagga gattatggag tagcaatacc acatacagat gcagaatata
    64561 ttaaagagca atttatatca gtctgtactt ttaatgaacc agtagtgttt agttctatgg
    64621 aagaccaaga tgaaaaagta cctgtaaatt tggctttcgt attaggactt aatcaaccac
    64681 acagtcaatt atctgtatta acagaattaa tgggaattat gcaaaataaa gagttagttg
    64741 ataagttgat gagttctact gataaagaag aagttttaaa gacactaaag tctttataaa
    64801 aataaaaaag tactaaaaat aaaaaatata aaaataaaaa ttattaggag gaaataaaaa
    64861 tgaaaaaaat attagtagca tgtggagcag gaatagcaac atcaacagta gtttgtgaca
    64921 aggtagagag attagttaag gaaaataata tacaagcaga agttatacaa tgcaaaatag
    64981 ctgaatgtgc aacaaagcaa gaaggagcag atttaatagt atcaacaact atactaccaa
    65041 caacttataa tataccagca ataaaagcaa cagcatatat aactggagta aatacagcag
    65101 cattagataa aaaaatatta gatgctttaa aataatccta tcatagatac attgaaataa
    65161 tattttataa aacagaaaaa ggaggattta taaatggata aattattagc agtagtacaa
    65221 tacattttaa atatgggacc gacagtaata ctaccaataa caattgtagt actaggaatg
    65281 atatttagaa ttaagtttac aaaagcttta aagtcaggtc ttacaattgg tataggattt
    65341 gtgggtataa acttagttgt aaacctatta acatcaacac tagggccagc ggctcaagga
    65401 atggttgaaa gattcggact taatttaaca gtaatagatg ttggttggcc aacaacagca
    65461 tcagcaacat gggcttcacc agttgcacca atattaatac cagtttgttt attagtaaac
    65521 ttactattat tatacttcaa taaaacaaag atattaaacg ttgatatatg gaattattgg
    65581 cactttatag tagcaggtgc agttggatat atagtaacag gaagttatgt ttgggctata
    65641 ttctgtgcaa tactaatgga gttattagta ctgtttattg cagaaaagac agcaaaaaat
    65701 gttcaggagt tttatgattt agaaggtata gcattaccaa ctggttcaac agcttcattt
    65761 gctccacttg gatatttagt aggtaaattt gttgaaaaaa taccaggaat aaataaaata
    65821 caagcagacc cagactctat acaaaaaaga tttggtatat ttggagaacc aatgatgatg
    65881 gggattatac taggtataat acttggagca cttgcgggat atgatgtagc aggaatattc
    65941 aatgtaggta tatcaatggg tgcagtaatg ttacttatgc ctagaatggt tagaatatta
    66001 atggaaggtt taattccaat ttcagaatca ataagagatt tcttacaagc aaaatatgga
    66061 gacagagaac tttatatagg tctggatgca gcagtagcaa ctggacatcc agcagttata
    66121 tcaacagctc ttatacttgt tcctataact ttgttcttag cagttatact accaggaaac
    66181 aaagttttac catttggaga cttagcgaca ataccatttt atatggcatt tatagtgtgt
    66241 tttagaaaag gtaatatagt tcaatcagtt ataacaggaa caatagtaat agctatatca
    66301 ctttatatgg ctacaaactt tgcagatgtt catacacaat tattacaaca agcaaatatg
    66361 atgccagaag gaacaacaat ggttacaagt atagatacag gtggaaactt gctaaaatgg
    66421 ggaatattga aattatctca attagtttct tcatttggaa tattctagta tgtagttaat
    66481 agagattact cttttttagt aaagtatgcg aaaatttaac atttagcagc tactaaaaaa
    66541 gagtaatatt ttttataaat ttttaaaaat acaaaataca agaggaggag atttatcgtg
    66601 aaagcagcag tattacatgg cacaaatgat atgagatttg aagatataga aataaaacca
    66661 tgtgaaagtg atgaagtaaa aataaaagtt atggcagcag gaatatgtgg gtcagatcct
    66721 ccaagagtat taaaacattg gaaatatcca gttccagcta taccaggaca tgagttttct
    66781 ggagtaattg ctgaagttgg taaggatgta aaaaatgtaa aagttggaga tagagtagtt
    66841 gcaattcctt ttataccttg taatgaatgt gaatattgta aaagaggatt attttcattg
    66901 tgtgatgacc atggaatgtt aggagctaaa agttttggag cttttgcaga gtatgtaaat
    66961 ataaaagcta caaatgtact tccaatagga gatatgaact ttgaagatgc tgcaatgata
    67021 gagccactag cagttgctat gcatggagta cttaatatag gtgttcaagt tggagacact
    67081 gtagctgtaa tgggtagtgg aacaatgggg cagttggtaa tacaaggact taaaatagca
    67141 ggggcaggaa caattatagc agttgatata tctgataata aattaagaga atcaaaggaa
    67201 ttaggggctg atataataat taatgctaaa gatattaacc cagtagaaaa aataaaagaa
    67261 cttactggtg gaaaaggtgt tgacatagct cttgaatgtg caggttcaaa aataactcaa
    67321 gaacaatgtt tacttataac taaaaagaaa agtaagatag gatttttagg gatagcatat
    67381 tcagatataa ctttatcaga agaagcattt gaaaatatat ttagaaaaga actagaatta
    67441 aaaggttttt ggaactctta ttctgcacca tttccaggac aagaatggac taagggaatc
    67501 aatctagtta atgaaggaaa gataaaatta aaagaaatgg tatctcatag attctcactt
    67561 gaagatactt acaaagcatt tgaaatgata agagatagaa aagaagaatt taacaaaata
    67621 ttaattttac cacaaggggt tgaaaaataa tgaaagcagt agttaaaact aaaccaggct
    67681 atgataatgt agaagtttta gaagtagaag aaccaaaagc tacaggagac aaggttaaaa
    67741 taaaagtaga atatagtggt atatgtggtt cagatataca ttcatttaaa ggtgaatatg
    67801 caaatataaa agccccagta acattaggtc atgaattttc tggaattgtt gtagaagtag
    67861 gagaagatgt taaaaatata aaagtagggg atagagttac atcagagact acttttgcaa
    67921 cttgtgagaa atgtatatat tgtgaaacta aagattataa cttatgccca actagaaaag
    67981 gtattggtac tcaagtaaat ggaagttttg ctgagtatgt attatcaaga gaagaaagca
    68041 tacatgtact tcctgaaaat gtatctttat tatcagcagc ccttacagag ccactagctt
    68101 gttgtgttca tgcagctctt gaaaagacta ctatagaaag ttctgataca gttttaataa
    68161 taggaccagg accaattggt ttattattag ctcaagttgt taaatctcaa ggtgctacag
    68221 ttataatgtc tggtgttaca aaagataagg aaagattaga aatagctaaa aatcttggag
    68281 tagatagaac tgtaaatgta atggaagaaa gtttggcaga tgtagtgaat gaaatgactg
    68341 atggaaatgg agtaaacaaa ggatttgatt gttctgggtt tatgccagca gttaacgaag
    68401 ccttaagact gcttagaaaa aaaggtactt tcgtacaagt tggaatcttt gctaaaaaat
    68461 taaatgaaat ggaccaagaa gcaataatcc aaagagagtt acaatatata ggaagcaggt
    68521 ctcaaaagcc aagttcttgg attacagcat tagatttaat ggcaaatggg aaagtaaata
    68581 cagaagcttt agttactaga actgttggtt tagacgaatt taaagaaggt attacagctt
    68641 taatgaacgg ggaagaaata aaagtagcag taaaatctta aaaaaacatt tgtattattt
    68701 taaaaaaaat tgtaaaatct actgtaggat aaaaaaatgt aaggtcttaa aaaataacag
    68761 gaggaagaaa gtatgagcac tcttagagat cattctaata atattagaaa aaacataata
    68821 aaaatggttg cagaagctaa aagcggacat ccaggtggtt cattatcaat agctgatatg
    68881 ttgacagtat tatattttga taagatgaac gtatgtgcag aaaatccaaa aatggctgat
    68941 agagatagat ttgttctatc aaaaggtcat gcagcaccag ctttatatgc tacattagca
    69001 gaaaaaggat ttttcccaga agaagaatta ataacactaa gaaaatttgg ttctaagtta
    69061 caaggtcacc cagacatgaa aaaagtagca ggaatcgata tgtctactgg ttctcttggt
    69121 caagggctat ctgcagcaaa tggtatggca cttgcaggaa aattagataa caaagactat
    69181 acagtatata ctatattagg tgatggagaa atccaagaag gtcaaatatg ggaagctgca
    69241 atgacagcat cacattacaa attagataat ttaattgcat ttgttgactt aaatggatta
    69301 caaatagatg gttctaatga agaagtaatg aatgtaagtc cagtagatga caaattcaaa
    69361 tcttttggat ggaatgtaat agtaataaat ggacattctt ttgaagaaat aggaaatgca
    69421 gttgatgaag ctaaaaaagt tacaggaaaa ccaactgtaa taatagctaa aacagtaaaa
    69481 ggtaaaggtg tttcatttat ggaaaataat gctgcatggc atggaacagc tccaaatgca
    69541 gaacaagcag agcaagcatt gaaagattta gaaggaggtc tataggaatg ggaatagcaa
    69601 ctagagaagc ttacggacaa gtgttaaaag aacttgcaga aaataaagat atagtagtat
    69661 tagatgcaga tttaggaaaa gctacaaaaa gtatatcatt taaagaagtt gccccagata
    69721 gattttttga catggggata gcagaaggtg acatgattgg tacagcagca ggtcttgcta
    69781 cttgtggaaa aatacctttt gcgagtactt ttgcaatatt tgcagcagga agaggctatg
    69841 agcaaataag aaactctgta gcatatccaa atttaaatgt aaaaatagca gctactcatg
    69901 caggtgtaac agtaggtgaa gatggtggta gccatcaagc tatagaggat atatctctaa
    69961 tgagaggtat accaaatatg gttgtattaa atccagcaga tgctttagaa gcaagacaag
    70021 ctattcttac atctattgat tataatggac ctgtttatat aagattaggt agagctgcaa
    70081 ctcctgatgt aaacagtgaa aattataaat ttgaaatagg aaaaggaact gttttaagag
    70141 aaggttctga cataacagta atagctacag gaataatggt agcaaaagcc ttagaagcag
    70201 cagaagaact tgctaaagaa ggtataaatg tagaagttgt taatatatct acaataaaac
    70261 cattagatga agcattaata aaagaaactg ctaagaaaac tggaaaagta gttacagcag
    70321 aggagcacag tataataggt ggtttaggtt cagctgtatg tgaagcttta gcagaaacta
    70381 aagatgtagt agttagaaga attggtgtta aagatgtatt tggtcaatca ggtacaccag
    70441 ctgatttatt aaaacactat ggtttaacta cagaagatat agtaaaaaat ataaaagagt
    70501 tgttatagat ataaatatat ctaaataaca aattgatttg acataagtca attaaagctg
    70561 tcttaaaaat agaaagaaat ttctacaatg ggacagcttt atttttacaa tactttattt
    70621 ttatgataat tataaaatag tctgaaagta atcaaaaaaa ttataaaaag aggtgctatt
    70681 taatgaaaat atctattgga tgtgaccatg gtggttatga attaaaggaa tatataaagg
    70741 aaaatttaat aaataaaggt atagaagttg aagatgtggg tacaaattca aaagaaagtg
    70801 tagattttcc aatatatgca aaaaaggtaa gtcaaaatgt tcaaagtaaa aaatctgatt
    70861 taggcatact ttgttgtgga acaggaatag gtatgtctat tgcagcaaat aagtttaaag
    70921 gaatcagagc agctgttgtt tctgactgtt tttcagctaa ggcaactaga gaacacaata
    70981 atacaaatgt actttgttta ggagaaagag ttattgggaa aggattagca atgagaattg
    71041 ttgaagaatg gttaaattca agttataatg gagaaagaca tgcaagaaga ttacaaatga
    71101 ttgatgatat agaaaaagaa aatttataaa tatataattt acaacaaggg ggagcgttat
    71161 gtctattatt tgtgcttcaa taatgtgtgc agaccaaatg aagttaaaag aggaattaga
    71221 agctcttgaa gaagcaggtg taaaattact tcattgtgat gttatggatg gaatatttgt
    71281 taaaaacttg gctatgggac cagagttatt aaaatcaata agtgaaaata caacaatacc
    71341 tttagacata cacttagcca cagaaactcc agataaatat attgatatga tgtcttatat
    71401 aaagccaaaa tatatttctt ttcatgtaga atcaagtaca aatgtaaaag ctgatataca
    71461 aaagttaaga aattatggta tagggccagt gttagcaata agccctcaaa caagtgtgga
    71521 taaaatagaa gaatatataa gtttagttga gggtatctta gtaatgacag taaatccagg
    71581 gtttgcaggt caaaagttta atctttctgt attggataag ttggataaac ttacagagat
    71641 actaaaagat tatgataatc ctccatttat agaggttgat ggaaatataa ataaggatac
    71701 tattaaactt atgaatggta aaaaagtaga tatatatgtt gttggtactt cagctttatt
    71761 taatgataag cctccaattt catataaaga taaaatagaa gaactaaaag aaagtataaa
    71821 gtaaccatgt aataaaatta taatatgttt ctgtttaata gtaaaaattt aggtggaaac
    71881 aagaaatttt ataatattta ataaaaattt tggttgagta aggagaattt tttatgggag
    71941 acctatataa actacaaaat ggtactgata ttagaggaat agcgtatgaa aatgattcta
    72001 aagaagttaa cctaactgta gaagaagtaa aaaaaatagc taaagcgttt catatttggt
    72061 taaaagaaaa gactaagaaa gataaagtaa ctgttgccat aggaactgat tctaggataa
    72121 caggaagcca atttagaagt actgttattg aaactttaac taatgatgat tgtaatgtaa
    72181 ttgattgtga gattgccaca actccagcta tgtttatgac aactattatg gatggataca
    72241 attctgatgg ggcgattatg attacagcaa gtcacttacc ttattactat aatggtttaa
    72301 agtttttcac agaaagtgga ggtttagaaa aaactgatat aaaagagatg ttggatatag
    72361 ctgttaaaaa tgatagtgta gactatgagg aagtaaataa aaaaggtgaa gttatagtaa
    72421 aaaatttaat agaagattat tctaatttat tgatagataa aattagaaaa ggtgtaaatt
    72481 caagtaaaaa ttatgaaaag cctttttctg ggcttaagat attggttgat gcaggaaatg
    72541 gggctggtgg attctttgct gaaaaagttt tacacatact gggagcagat acgacaggta
    72601 gtcagttttt gaacccagat ggtatgttcc caaatcatat acctaatccc gaaaacaaag
    72661 aagcaatgga aagcatatgt aaagctgtgt tggataataa gagcgaccta ggaataatct
    72721 ttgatacaga tgtagacaga gctgctattg taggtaaaaa tggcaaacca ataaataaaa
    72781 atgctcttat agctgttata tcatcaatag tattagaaga acatccaaaa acagctatag
    72841 taacagattc gattacttct gaaggacttg ctaaatttat aaatgaatta caaggaagac
    72901 atcatagatt taaaagagga tataaaaatg taataaatga agctataaga ttaaatagtg
    72961 aaggtgaaga gtgccattta gctatagaaa cgtctggaca tgctgcttta aaagaaaatt
    73021 atttcttaga tgatggggca tatttaatag ctaaaatttt aataaaagta gcaaagctat
    73081 ccttggaagg aaagactata gaagaattga tagaaaacct tgaagaagct caagaagaga
    73141 aagaagttag aataggcatt aataaagaag attttagaac atatgcagag gatatattaa
    73201 aagatttaga ttctcatgtt aagagtattg atggatggtc tgtagcacca aataattttg
    73261 aaggcataag agttaattgt gatgaatcaa atggagatgg atggttttta cttagaatat
    73321 cactacatga accattatta gctttaaata ttgaatcaaa taaaattggc ggagcagaac
    73381 aaatttattc taagttaaaa cttttcttag aaaaatatga tttaaaaggt ctttagggta
    73441 aatagaaaga atatatttat attgaagaat aaatgtaaat ttgtacctaa gattacaaat
    73501 attacaaaat tgtaatctta ggtaacagag atgtaactga ttatggaaac actatgtgat
    73561 attatagata cagagagatg aaatatgtga tgaattatag agaaatataa tttaaaatgt
    73621 attaatatca caccaaaaag gtataatcaa aaaatatgta aatataaacc ccaacattta
    73681 gaaaaagacc tctaaggagg tttttttctt gtttaaataa cttaaaatat atttttatat
    73741 taatatatca attaactttt ctaaaagtaa gcattacctt aaataaatca gcatctataa
    73801 ttatttccat gtgtgcattt tctaattcaa ttatattttg agcaattgat aaaccaagac
    73861 cagagccatc tgtttttcta gatatatcac cccttctaaa tctttctatt aactcttctg
    73921 gttttagatt tagtttatca tttgaaatat ttttaaacgt taatattata ttttcttcct
    73981 ttataaacat atctatatag accctagtgt taggcataga gtattttagt atattgcaaa
    74041 ttagattctc aaaaactctc caagtttttt taccatctat catggtaaaa acagcatatt
    74101 caggtgtttc tattataaaa tctaaactgc tattttgaat tttgtcttca aattctacaa
    74161 tactttgaat taaaagttct cttatatcgg tttttgttat attaaattct acatctccac
    74221 tacttagctt agatgcttca aatatgtcat ctattaataa ttttaatctt tgtgattttt
    74281 tatctaaaat atttatatag tcatttatag tctctctgtc aaagtcatta ttttttaaca
    74341 tatcaacata gcttaatata gatgtaagtg gagtttttaa gtcatgagat acgtttgtga
    74401 taagctctgc cttcatcctt tcatttttta tattagcatc tacagcagtt tcaagaccgc
    74461 ctgttatatt atttatactt ttggctaaag tagatatttc atcatctcct ataacttcta
    74521 atttatactt taaattgcca ttttctatat tttttatatt ttttgatatg ttatttatgt
    74581 aattaaactt aacagataaa aagttgtata tataaaaatg aaaaactgtg tttacaagtg
    74641 taaataaaat tactatagct attgcctcag cataatatat atacataaag aatatcaaaa
    74701 caagatattg gataatagct tgtacaacta tagatattaa aaatactata gctaattttt
    74761 tatttatagt ttttttatta tacatactaa gtaagtttga aaagaataca tatgttatag
    74821 acatatcttt tagattatag ttattatat
//
LOCUS       DAEPIN010000010        68710 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.010, whole genome shotgun sequence.
ACCESSION   DAEPIN010000010 DAEPIN010000000
VERSION     DAEPIN010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 68710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 68710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68710
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.010"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            complement(186..380)
                     /locus_tag="KR250_000825"
     CDS             complement(186..380)
                     /locus_tag="KR250_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0066790.1"
                     /translation="MFQVINFLCLFCVGFLVLDVMIYFCKDAMDIFEGTTNVNKVKSI
                     NVWADRVRANSYSYENKIAK"
     gene            complement(689..2218)
                     /gene="lysS"
                     /locus_tag="KR250_000826"
     CDS             complement(689..2218)
                     /gene="lysS"
                     /locus_tag="KR250_000826"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HBH0066791.1"
                     /translation="MKNNQQSNEEAQIQEDLSEVLQVRRDKLKKLQESGRDPFKESRY
                     DRTHYSMDIKDNFDSLEGKTTKIAGRIMSKRIQGKAGFIDIQDQEGRIQSYVRLDAIG
                     EEEYSVFSTYDIGDIVGIEGEIFKTKKGEISVKAKSVVLLCKSLQVLPEKYHGLKDQE
                     LRYRQRYVDLIVNPEVKNAFLIRTKALKALRAYLDDRGFLEVETPILNTIAGGANARP
                     FITNHNTLHIPMYLRIANELYLKRLIVGGFDKVYEMGRMFRNEGMDLKHNPEYTAIEL
                     YQAYADYTDMMEITENVIAHMAEVATGSMIVNYQGTEINFTPPWKRMSMEDCVKEYSG
                     VDFSTINTDEEALEVAREKGIEIKPGMRRGEVINAFFEEFGEDKLIQPTFITHHPVEV
                     SPLSKRNVEDPRRTDRFEAFANKWELANAFSELNDPIDQKGRFIDQLRKRELGDDEAF
                     EMDEDFLKALEVGLPPTGGLGIGIDRVIMLLTNSPSIRDVLLFPTMKPIDNNPNKEEE
                     N"
     gene            complement(2233..2712)
                     /gene="greA"
                     /locus_tag="KR250_000827"
     CDS             complement(2233..2712)
                     /gene="greA"
                     /locus_tag="KR250_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HBH0066792.1"
                     /translation="MEENKEFLLTQEGYDKLEEELENLKVVKRKEVAERIKVAISFGD
                     LSENAEYDEAKKEQAQVEERILKLENMVRKAVIIDESKIDLNVVTIGSIVKVKDLEFD
                     EEVEYTIVGSTEADPYDGKISNESPVGKALLGRAAKEVVEVQVPDGVAKFEILEIRR"
     gene            complement(2821..3789)
                     /gene="dusB"
                     /locus_tag="KR250_000828"
     CDS             complement(2821..3789)
                     /gene="dusB"
                     /locus_tag="KR250_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HBH0066793.1"
                     /translation="MKIGNLELKNKVFLSPMAGVTDLPFRLICKEQGCGLLYTEMING
                     KALCYDDENTKKMLKIEEEEHPVAVQIFGSEPEFMGRAAEIMNDYSNEILDINMGCPA
                     PKVVKNGDGSALMKNPKLAEEVLRAVVKNSKKPVTLKIRKGWDDNSVNAVEIAKIAED
                     CGISALAIHGRTREQFYTGKADWDIIAEIKKNLSIPVIGNGDVFTIEDSINMLDKTGC
                     DAIMIGRGAQGNPWIFKRINHYMNTGEILPEPTLNEKISTAIKHLKLAVEEHGEYVAV
                     REMRKHIAWYLKGLRNSAKLRDEINKIEDYQEVVSKLEYYMEDSLT"
     gene            complement(3926..4696)
                     /locus_tag="KR250_000829"
     CDS             complement(3926..4696)
                     /locus_tag="KR250_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HBH0066794.1"
                     /translation="MLLVFDVGNTNMVLGIYKGDKLVNYWRIKTDREKTSDEYGILIS
                     NLFDYDNVNISDIDDVIISSVVPNVMHSLENFCIKYCKKQPLIVGPGIKTGLNIKYDN
                     PKQVGADRIVNAVAGIEKYGAPSILVDFGTATTFCAISEKGEYLGGTIAPGIKISSEA
                     LFQSASKLPRVELAKPGMTICKSTVSAMQSGIIYGYVGLVDKIISMMKKELNCDDVKV
                     IATGGLAKLIASETKSIDYVDGFLTLEGLRIIYEKNQE"
     gene            complement(4715..5305)
                     /locus_tag="KR250_000830"
     CDS             complement(4715..5305)
                     /locus_tag="KR250_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBH0066795.1"
                     /translation="MMNTSASVRKVNVRKMTIIGVLSAISIMLSMTPLGFIPVGPTKA
                     TIMHIPVIIGAIMEGPIVGAAIGFIFGISSLLNAITNPTVTSFVFINPLVSILPRVMI
                     GVLAYYVYQLIIKATNKVYISGLITGAIGSLLNTAGVLGMIYVLYADKYLHAMGKSGS
                     AGKVIMALAAANGVPEAIVGALVVAAVATVLKKSKK"
     gene            complement(5489..6457)
                     /locus_tag="KR250_000831"
     CDS             complement(5489..6457)
                     /locus_tag="KR250_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HBH0066796.1"
                     /translation="MYNNILDIEGLKVGQVEDRNGLTGCTVVICEEGATCGVDVRGSA
                     PGTRETDLLDPVNMVQKVHAVVLAGGSAYGLESTCGVSRYLEEKGIGFDVGVGKVPIV
                     VGAVLFDLGVGSFKCRPDLKMGYRACEVANTDILNQGNYGAGCGATVGKIRGQEYAMK
                     GGIGSYSIKLDNGLIVSALIAVNALGDVYENGKVIAGALNDKKTETIDTYKLMKQGES
                     KGGFSVDNTTIGIIATNAKLTKAGCKKISQMGHDGYAKSIFPIHTPHDGDTIFTISTG
                     EIEADITLVGSLAVEVVEKSIINAVKKADSVENIMAYKDIVKKREI"
     gene            complement(6450..7427)
                     /locus_tag="KR250_000832"
     CDS             complement(6450..7427)
                     /locus_tag="KR250_000832"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HBH0066797.1"
                     /translation="MRDKIIEIILNNKSEFISGEELSKQLGVSRAAIWKHMKALKEEG
                     YNIESVNKKGYRLAENPTDLLSPQNIYYKLDTEFIGKNIEHLDTIDSTNEHAKRIAIN
                     SVDGTVILSEHQDMGKGRLGRRWDSKPHEGIWMSIILKPDMEPFKAPFITLIAGASVT
                     KALNNLGANTLIKWPNDVILNGKKICGILTELSAEIERTNYVVLGIGINVKIMDFEQE
                     IADKATSLYKEGYMLSRVDIVRNVLTEFEKLYLDYTVNNSKEKTLKICRDYSAIIDKN
                     IYVLKGDNRELVKCLDINEEGNLLVQRSDGSIKEIISGEVSIRGEKGYV"
     gene            complement(7742..9712)
                     /gene="ftsH"
                     /locus_tag="KR250_000833"
     CDS             complement(7742..9712)
                     /gene="ftsH"
                     /locus_tag="KR250_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HBH0066798.1"
                     /translation="MNKLLKGAGFYLLVFIIIVGIVQFSGKPTEKIKDLKFSEVYREL
                     TDENISRLYFVNQTSVEGTIKDTNTKFKSYVPTEVMGNKLADEVLDQAKAGKLTFGGE
                     AKPSTPWFVEMLPTLLLIFFMVIIWFVFMNQSQGGGGKVMSFGKSKAKVHKDDEKTRV
                     TFKDVAGLDEEKEDLQEVVDFLKNPKKYIELGARIPKGMLMVGPPGTGKTYLSRAVAG
                     EAGVPFFSISGSDFVEMFVGVGASRVRDLFEQAKKSAPAIIFIDEIDAVGRKRGAGLG
                     GGHDEREQTLNQLLVEMDGFGVNQGIIIMAATNRPDILDPALLRPGRFDRQVVVGTPD
                     VKGREAIFKVHSRNKPLSDDVKMDVLARRTPGFTPADIENLMNEAAILTARKREKKIK
                     METIEEAITKVIAGVAKKSKVISEKERRLTAYHEGGHAVCAHVLEEVSPVHQVTIVPR
                     GRAGGFTMQLPVEDKFYATKNEMKENIVVLLGGRVAEELVLKDVSTGASNDLERVTAT
                     ARSMVTKYGMSSKLGPMSFDSDDEVFLGNSFSSKRNYSEEVAFEIDQETKRIVDGAYD
                     KTRSILQENMDRLEYVAQALLIYETLDAEQFVKAFNKELPLNEIENAVTEENSSKEVE
                     EQLTIKLEKDEEERNNVIDINKNLEDKSDKDK"
     gene            complement(9746..11134)
                     /gene="tilS"
                     /locus_tag="KR250_000834"
     CDS             complement(9746..11134)
                     /gene="tilS"
                     /locus_tag="KR250_000834"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HBH0066799.1"
                     /translation="MIFDKVLSTINKHNLIQKGDKIVLGLSGGPDSVCLLHVLNRLKK
                     DFNIEIYAAHLNHQIRGIEAQKDALYVSKLCEDMGIIFFVKSINVPKYCENEGLSLEE
                     GARKLRYEMFYEIKDKIKANKIAIGHNLNDQAETVMMRIMRGTGLKGLKGIDYIRDNC
                     IIRPILDVERNEIEEYCEAYNLNPRIDKTNLENIYTRNKIRLDLLPYMKDNFNSNVIE
                     SIVRMSNSLKSDNDYIEKEAEAKFREVSNIKEKGFVEINLDDFVCLHDAIKVRVLRNS
                     IKHILGDTNFVDQRHIEDIMSLEDNSKVNKMLTLPRNIFVYRKKDSIILTNEEIVNEE
                     IEFYYNVPSNGFIKIKELKQIIETQVMSIDRYKSMKLDNSSKGFDFNKVKGGIVIRSR
                     RQGDKIKLAMGSKKVKDLFIDLKIPREERCKIPIITDSEGIICVGDYKISENYKIDEN
                     TKEVLKINFNKL"
     gene            complement(11317..11745)
                     /locus_tag="KR250_000835"
     CDS             complement(11317..11745)
                     /locus_tag="KR250_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HBH0066800.1"
                     /translation="MEEKSLSVKISINTRQYDEKGNMDTIEMTAFGKIFYKNDGIYVI
                     YKEKEEDIEITNTIKILKNQVSIKKFGAINSTMLFKCGESSTTKYVTPQGTLLIDIDT
                     RELDINIQEGEHIKLKIDYNIKVQDLFLGRNKIDIYIGTK"
     gene            complement(11762..12568)
                     /locus_tag="KR250_000836"
     CDS             complement(11762..12568)
                     /locus_tag="KR250_000836"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HBH0066801.1"
                     /translation="MNNKPIGVFDSGLGGLTVLKEIMKILPNEDIIYFGDTARIPYGS
                     RSKETIIKYTFQAINFLKTKGVKAIVIACNTATARSLKEAQEKYDIPIIGVIEAGART
                     AVSSTKNKIVGIIGTEGTISSKAYNLEISKIDESIEIVNKACPLFVPIVEEGWANTEV
                     AKLTAKIYLQELKEKNIDSLVLGCTHYPILKRTIGEEVGEHIKLVNPAKETAKDLKKI
                     LEVQNIINNTEIHGTYQYYVSDIPEKFSDIAREFLKKKIDKIQKVEIQKY"
     gene            complement(12770..13255)
                     /locus_tag="KR250_000837"
     CDS             complement(12770..13255)
                     /locus_tag="KR250_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HBH0066802.1"
                     /translation="MATLLALTFIFMPIKEVFAYEPTENIKKVNKQEKNIAKKEVINL
                     SDNDLMLLSKLVAGEARGESYEGQVAVAAVVINRVLDPRFPDTIEEVIYQKNAFSVVL
                     DGSIKMAPTDSARKAAQEALYGTDPTNKAVYFWNPEIATCKWINRLNPYMRIGNHVFA
                     K"
     gene            complement(13306..13965)
                     /gene="spoIIR"
                     /locus_tag="KR250_000838"
     CDS             complement(13306..13965)
                     /gene="spoIIR"
                     /locus_tag="KR250_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein R"
                     /protein_id="HBH0066803.1"
                     /translation="MRKIKIRLGLLILSLISVISIMTIVINGEVKKVDNISKDYKDKL
                     IRFHVIANSNTDEDQELKLKVRDEVIKYLQPKLQNSKSIKESEAIIKKEYSSLEEISK
                     NIILKNGYNYSVKVGIQYSNFPTKQYSNIVLPAGEYKALKIIIGKGEGKNWWCVMFPP
                     LCFVDESNGVIDKSTDDKLKEVLTDKEYKLIKQDTPKKTSRVKIKFKVLEVVKDLEKK
                     F"
     gene            complement(14110..14790)
                     /locus_tag="KR250_000839"
     CDS             complement(14110..14790)
                     /locus_tag="KR250_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBH0066804.1"
                     /translation="MENLTKLNLDNYKPLRDVVFENLREAILEGKLKPGQRLMEVQLA
                     EQLGVSRTPVREAIRKLELEGLVVMLPRKGAYVANMSLKDIMDVLEVRASLEGLAAYL
                     AAERISDEDIKKLKDISEEFKKSTLESDVDALLKLDVEFHECIFKATNNKKLIQLINS
                     LWEQVYRFRVTYMSAYDTSHNIVEEHKYLLDAIIKGDSELSKKYATEHIEKAEQFMID
                     KAMISKDL"
     gene            complement(14774..15664)
                     /locus_tag="KR250_000840"
     CDS             complement(14774..15664)
                     /locus_tag="KR250_000840"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HBH0066805.1"
                     /translation="MDFIRLKSRAKINLSIDVLGKRQDGYHFVEMIMQTIDLYDIVKI
                     KELDEDEIKVKSTSLDIPLDEDNIVYKAAKILKNKFYIKKGVEIFIEKNIPVAAGMAG
                     GSSNAAAVLVGLNHLWELRLSEDELKEIGLNLGADVPFCISGRPALAQGIGEKLTNIK
                     GLPCDTNILICKPDLFVSTKEVYQGLDLNNIKKRPNNKYLIECLKSEDIKAVSESMVN
                     ILENVTIGKHKEISDIKQVMMKNNALGSMMSGSGPTVFGLFKNKEDALIGKKELLKKY
                     KQVYVVNSSQKGVEICGEFN"
     gene            complement(15782..17332)
                     /locus_tag="KR250_000841"
     CDS             complement(15782..17332)
                     /locus_tag="KR250_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3794 domain-containing protein"
                     /protein_id="HBH0066806.1"
                     /translation="MELIKDVIKVDNRIDFGKFQTFIEAEAVVPDKKLDVYEIVKTEG
                     YISLKKIEMADGKILCRGSFNYNVIYIADDKNTISNVDGKVDINEVIEKDNVVQDMEY
                     MLFSEVEHMDCTIMNERKIKVGALMNIRGSLFEKQRLDIVKDVAQVEGIQKHRKEICF
                     QDIVGIEKAESSIRDTITINTEEIQSIISLNPCVKVKESRVTDNKVIIGGVLEINPLA
                     CTYEGELVELDRVGIEFTQFVEVPGVSDGMTEEVLLSMSDFNHIFKQNSESNTGLLEI
                     DCMACCKVKVTDEVTREVLQDAYSPQKIIKFDHKPIQLNKTLRRSEETFMVREGIRND
                     NDDIQIKDIVSVCPTMSIENSYIEGNKSIIQGIIKVEILFVPVEGLKLVYKISEEIPF
                     EHDIEMDNLTDTASVFNTACIDKVEVDLNRDQIDLIIKIKRFTEALDKKAENFIIKGE
                     DQGVYDLSKAPSIIVYICKEGDTFWNIAKKYNTTENEIAELNDIKLDEPIKPGKCLIL
                     EKKVVLVD"
     gene            complement(17426..17692)
                     /locus_tag="KR250_000842"
     CDS             complement(17426..17692)
                     /locus_tag="KR250_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HBH0066807.1"
                     /translation="MATVQTLDKIRVSLERHIGKKILLKANKGRKQIITKEGILEKVY
                     PSVFVIKLDDESNGYPRVSYSYSDLLTSNVKLQVFRDQDKLQIS"
     gene            complement(17840..18700)
                     /gene="yabG"
                     /locus_tag="KR250_000843"
     CDS             complement(17840..18700)
                     /gene="yabG"
                     /locus_tag="KR250_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation peptidase YabG"
                     /protein_id="HBH0066808.1"
                     /translation="MKVGDIVARKSHNKDIVFKIVSFGVDENNEKIAILKGIAFRVIA
                     DAYIDDLELVKAPDIKDILIDKNVENLLYKSVRKAKERQKRMTRAVPKLQLNPNTYGM
                     PGKVLQIDGDKEYLKICLDVYAQLGIPAVGVAVSEQNQYKEVRALLEKYNPDILVITG
                     HDAMTIKRGDIEDMSNYRNSSNFVKTVKEARKWQPNLDNLVIFAGACQSNYEKIISAG
                     ANYASSPGRIMIHALDPVFIVEKIACSRIDVVVPIDEVIEQTITGVKGIGGSETRGKF
                     RWAMPKTILH"
     gene            complement(18784..19722)
                     /locus_tag="KR250_000844"
     CDS             complement(18784..19722)
                     /locus_tag="KR250_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HBH0066809.1"
                     /translation="MKFIDLHCDTIAKLMENVETSELKSNKYSVDIDRLKKGDSLAQT
                     FALFVDTEEVKHPFDYCMSMANKFHEEMKKNSDEIALATNYEEIMKNQSEGKLTALLS
                     IEEGAVLEGKLENLKKFYDLGVRMMTISWNHVNELSFPHNKVEYREKGLTDFGREVVH
                     KMNELGMLVDVSHISDGGFYEIAKISSKPIIATHSNSRAMMNHSRNLTDDMIKVLANK
                     GGVTGINFFHLFLSDKSESKLEDMVRHIKHIVNVGGIDVVSLGSDFDGIDSKVEIEDI
                     SQMGKLYEPLKKEGFSEDDIEKIYYKNALRVIKEVL"
     gene            complement(19832..19993)
                     /locus_tag="KR250_000845"
     CDS             complement(19832..19993)
                     /locus_tag="KR250_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0066810.1"
                     /translation="MFILRFADDDDNLSVKDFTSLTDLKKYISENNIDKTWHQIEEVK
                     KVIPNLKEE"
     gene            complement(20003..20500)
                     /locus_tag="KR250_000846"
     CDS             complement(20003..20500)
                     /locus_tag="KR250_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C domain-containing protein"
                     /protein_id="HBH0066811.1"
                     /translation="MSLYTEWRNLSDNHESQEAEVKFWEEYLKVEAGIYNEILNNKMD
                     VVEGKVSELAEKFGTTNEFFMGFIDGISESLKEDIVLEEVEADKEVSIKIDWEKLYNN
                     MVTVEAHWLYNLQGWEDILPADQRKEIIKAYKKSKTIVKEAKIGRNDSCPCGSGKKYK
                     KCCGK"
     gene            complement(20615..21325)
                     /locus_tag="KR250_000847"
     CDS             complement(20615..21325)
                     /locus_tag="KR250_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0489 family protein"
                     /protein_id="HBH0066812.1"
                     /translation="MENYEYSGFYIEKPVGNNVFSYDKRENKSIYVPKLINGTLNDVR
                     LGNEVVFNEVDENKEIKAKGLENMVEYVLGNKKIYVFDNHNHAFYFWAKSLLKGEFTK
                     GCKLVHVDQHKDTREPENYDVDVNNLKDVFRYTNEVLNVGSFIKPALKYNIFSELIII
                     DSLYGFDLEVESEFVLDIDLDIFSSDMDYIPFELKFYKIKNLIKKAKVITIATSPYFI
                     NQEYAIKVLKELFNYDII"
     gene            complement(21501..22031)
                     /locus_tag="KR250_000848"
     CDS             complement(21501..22031)
                     /locus_tag="KR250_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0066813.1"
                     /translation="MDIRALPIRITKEEAIEIAKSGKNSLFSKLILKNKYAHEVRLHY
                     IEFKIITLKIHKTKSIFKNRRYSNDINELKILVNGSTGSGSIVQDFPTYEVVKNVDCN
                     IVQYSDKDERRVKAKANKMAMRLTHRFMGGIPEIEIVKIESIFRPYWVVFFGKVELNR
                     RVSYLPIEADGFVIGM"
     gene            complement(22048..22278)
                     /locus_tag="KR250_000849"
     CDS             complement(22048..22278)
                     /locus_tag="KR250_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0066814.1"
                     /translation="MSKLVSKVKKDLEIKWLGIDGFLVLLYFLITGFIFYGILTRNDI
                     IVGKIIPYGVMSVVIVIFFDYIYRVLKMTRKK"
     gene            complement(22293..23687)
                     /locus_tag="KR250_000850"
     CDS             complement(22293..23687)
                     /locus_tag="KR250_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HBH0066815.1"
                     /translation="MLTFWENGIIVIFAFILLGIGYYFSRNVKDMESYYLGNRSLPWS
                     LIVGTLVASWYGGVGTVGSVEYAAIYGISAWAVWSIAAHIGRMPLALWVGPRIHIRTN
                     ITVPDLLESFYGKHVAIIGAILMFIYCAQLGEITAMGTIGNVAWKIDKELIGLIMVGV
                     VVVLTVLGGLMGVAITDMVLFFCMVFGLTMVMPGQFEQIGGFQGLTEALKDAPQLMHP
                     TKGMSAMKALMLIVYSFGAYSDPTFYQRFSAADSPKVGRRALLTCFSLWICFDMVLTM
                     TGLIVRATYPEMAPAAGYITLVLDTLPQGIRVIFIIGILGSIISTLDSYYLAAGATLA
                     NDIYGRITKKELSQKQLVKFTRIGVCLAAVMGLSVAFRFENVYDACIFVGSIWMGSAF
                     VPIVGGLLGNGRRTSMGGILGMIVGGVTFGYFKIFPVQSFELEPLVIAIPFSFIAWMI
                     GNRIGENLNTQNNL"
     gene            complement(23813..25066)
                     /locus_tag="KR250_000851"
     CDS             complement(23813..25066)
                     /locus_tag="KR250_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBH0066816.1"
                     /translation="MESKNNVKRFLIIFILAFGTTAMYSLPYMKSSFYDPMQQALALS
                     HTQIGNLLSLYGLVGMVSYFIGGWFADRFSVRKLITFSLIASGILGFYFSTFPSYSMI
                     LLIFVLWGFTTILTFFSASVKVVRMQGSESEQGRIFGFYEGLSGVSGTLISFIGLYFF
                     GKFAEITVGFKYVVWLYSAASIICGILLFFLVEEKKDSGASDEGLSIKSLLKVVTMPK
                     AWLIGLIIFSTYLVFSSLTYLSPYLSEVYVMPMTLVSALSIIRTYVIKMGASPVAGVI
                     TDKVGSSIRVMFVGFILMTVSTAAYLVIPKSTGFIWIAVINMIILSVILFGFRGIYFA
                     SVSESNISLETTGAVVGFASFIGFSPDAFYYTIAGNWLDKYGQTGYTYIFILSVVCAV
                     IGIFATYALNKINKRENKGLNNKIA"
     gene            complement(25117..26271)
                     /locus_tag="KR250_000852"
     CDS             complement(25117..26271)
                     /locus_tag="KR250_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBH0066817.1"
                     /translation="MSNNINQDLETKIIHWGHSADPTTGALATPICQTATFAAKTVEH
                     FEELCMTWGYVYTRECNPTLTELEAKLAMLENAESAISSTSGMGAITSTILALVKSGD
                     HIVSSDGIFSHTKLFMSELLSKFGVEVTFVDAVNPQNVKEAMRPNTKIVYIESPLNPS
                     LDLVDIKTIAEIAHENKSLAIVDSTFGTPIVQRPIDLGADLVIHSLTKFINGHGDTLG
                     GAVAGSKELIDLVRWPSLCCFTGASLPPMSAWMILRGMKTLDMRMKKHCENGLAVAEF
                     LEEEENVELVKYPALKSHPQYELCKTQMNGLGGGVVSFKLKDGINGLTRDQASRRLMN
                     SLELATIATSLGEEHTLVQMNGENLIRIAVGLESSNDIINDFKQAIEKLK"
     gene            complement(26831..27400)
                     /locus_tag="KR250_000853"
     CDS             complement(26831..27400)
                     /locus_tag="KR250_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBH0066818.1"
                     /translation="MIIIYGYCRVSTKNQTDNNSLYQQQQEILNKYNDAKIIKETYTG
                     TTTNRPKFEKIISSLKKGDLLVATKLDRLARSTSEGIQLIEGLFAKEVSVHILNIGLL
                     ENTSMGKFFLTTLLAVAELERNMIIERTQLGKEFAKKKKDFKDGRPKKYSNQQIQYAL
                     GLLNSKPYREVVKMTGISKSTLIRAKKSI"
     gene            27603..27830
                     /locus_tag="KR250_000854"
     CDS             27603..27830
                     /locus_tag="KR250_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBH0066819.1"
                     /translation="MNSLLKEAIDELKNIHCGEVFLLKELFKGYEWNRLEMNIRLKLG
                     KLFLYEVVTNPNLNVEVINKNSAYQQMYKKK"
     gene            complement(28049..28792)
                     /locus_tag="KR250_000855"
     CDS             complement(28049..28792)
                     /locus_tag="KR250_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HBH0066820.1"
                     /translation="MENYQSTNTCTYNILYVQFNEDFERSKYFSYDVKYPVIYNIRNQ
                     STYVNPVILNNINNAVRMTVNNFKNGLQEEEREYNNATSQNSLPKQSYWVSTSYAVTF
                     NKNYVLSMILNLMGFANNYGPKYNTLNNYNIDLTTGKELLLNDIFRDGVDYINIITDY
                     IKSQINENREMYYSNVELVIPEDQAFYLTDNGIIIYFGVDEIAPESFGIPKFELKFSD
                     FGNFINLRFYCISPDIYVQSRGRAKHFMG"
     gene            28960..29832
                     /locus_tag="KR250_000856"
     CDS             28960..29832
                     /locus_tag="KR250_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBH0066821.1"
                     /translation="MLLLADKWKDYELIDMGNGEKLERWGSYVLRRPDPQVVWPMESE
                     WALWKNPHGHYHRSNKGGGQWEHKKRYPEQWTINYKNLKFHIKPTGFKHTGLFPEQAA
                     NWDWMIEKIKKANRPIKVLNLFAYTGGATVACASAGAEVCHVDAAKGMVNWAKENIEL
                     SGLKNQTVRFIVDDVVKFVEREIRRGKKYDAIIMDPPSYGRGPKGEVWQIEEKLYSFV
                     DLCMGVLSDNPLFFLINSYTTGFSPIVLENVLSTTIGKKTNTGDIYGGELGIPASRDG
                     KILPCGIFGRWEKK"
     gene            29832..30497
                     /locus_tag="KR250_000857"
     CDS             29832..30497
                     /locus_tag="KR250_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBH0066822.1"
                     /translation="MIKVIYEDNHLLVVEKPVNILSQGDNTNDKDMVNLLKDYIKKKY
                     NKPGNVYIGLIHRLDRPVGGVMVFAKTSKAASRLSEQVRSKTFKKTYLAVVHGKIKSN
                     SDILKDFLYKNKKTNMVTVVKKSHKDAKEAELNYNVLDFKENLSLVQVNLKTGRSHQI
                     RVQFASRKHPLYGDQRYGKEINKIGQQISLWSNKIEICHPTTKEKMEFTCHPPDKYPW
                     NIF"
     gene            30518..31375
                     /locus_tag="KR250_000858"
     CDS             30518..31375
                     /locus_tag="KR250_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBH0066823.1"
                     /translation="MSKKFSIGEMSKLHKISIQTLRYYDQIDLFKPVHVDQESNYRYY
                     SIDQFSQLDIIKYLKYLGMPLKEIKSKLDENNEEILKLLTNKINIVDEKIKELELIKK
                     VLSTKKETMEKGMKVDEVGKIVRKHIPRRSILSIEYEEGLSSEETMELSRRQIANILE
                     DNISVFYGGVSGLVSIDKFLKEDKVIYDNSFVTVERDLFDIKSQKHITEISSGDFICM
                     TYKGPYKENYTAFKKLVDYVEYKRIPIENYIYEIPIIDPLSTNNEEELLTEIQIQVKK
                     REIDLRCLV"
     gene            complement(31531..34077)
                     /locus_tag="KR250_000859"
     CDS             complement(31531..34077)
                     /locus_tag="KR250_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBH0066824.1"
                     /translation="MNIFNKVALQGLKKNRTRTLVTIIGVVLSAAMITAVTTFGVSLM
                     NYMTNGAIIKYGDWHVQFLDVDSSFVQEQRVNDEVANTATFENIGYATLEGNRNPDKP
                     YLFIAGFSKEAFDTLPITLISGRLPENSREILVSSKVATDGGVKFAVGDTISLDVGKR
                     MSENEKLGQSDPYTAGSEVFVPQDKKTYTVVGICRKPVFEESSAPGYTLITKEDKQVK
                     TDSLSLFVTLKNPHQIRSYISSTKDHASIFNDNVLRFIGVSDNNLFNMFLYSVGGIVL
                     AIIMLGSIFLIYNSFNMSLNERTHQFGILSSVGATAKQLRNSVLFEGICIGAIGIPIG
                     VAIGIVSIGLVISVIAKNFANILYVNVPLTLTLSIPAIIVAAVVSMVTILISAYIPAR
                     KAANTPVMECIRQTNEVKIESKSVKTSKLTQRVYGLEGVLAMKNFKRNKKRYRSIVLS
                     LVLSIVLFISTSALVADLKQMSNQRSEVTDQDIIFSTQGMKDNEMLKLYDKLKTANGV
                     YEGLYQKLMTYSCVAKVSELTDDYLNYEGSHSMSEMLELPVDMQFLDDRTYLNIIKSL
                     GLPAEEYTGQNAKLIAVSMVTKDNNIIDIFNSSSMNVSLTPETNGKLEIPERQNVNIS
                     FVKVVPPDSPPGIRSSDVKKASFRVLAPYSAKDKFEIPGVSTGIESLSFQSKNPTQSV
                     AEMEQIIESEGVTNEYNLYNMYGMMDENRNYIFIANVFAYTFIIMISLISIANVFNTI
                     STNIKLRRRELAMLRSVGMSEHDFQKMMNFECAFYGMRALFFGLPIAVISSWLIHKVM
                     VTDDIDFVIPWFSIGISIFSVLFIVFITMLYTINKIKKENIIDALRDDMA"
     gene            complement(34077..34763)
                     /locus_tag="KR250_000860"
     CDS             complement(34077..34763)
                     /locus_tag="KR250_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0066825.1"
                     /translation="MAFLKIEDLCKVYGKNENKVTALDHVSLTIEKGEFTAIIGSSGS
                     GKSTLLHSIAGVDVPTSGKIYLEGQDVYGQSNEKLAIFRRRQVGLIYQFHNLIPTLNV
                     VENITLPILMDKRKVNQERLNDLLELLGLKERKTHLPNQLSGGQQQRVAIGRALMNAP
                     AVMLADEPTGSLDSKNGQEIIQLLKESHSKYHQTLIIVTHDENIALQADRIICISDGK
                     VVRDERKVNR"
     gene            complement(34846..35835)
                     /locus_tag="KR250_000861"
     CDS             complement(34846..35835)
                     /locus_tag="KR250_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBH0066826.1"
                     /translation="MFRNREFRKFAILFLLITILTVALGFAISIMTGILSIVSATTFG
                     IAFFVFTKNRYKSIAQISEQINLVLHNANHLYIAESDEGELSILQSEITKMTLRIREQ
                     NYALKKEKEHLADSLADIAHQLRTPLTSVTLILSLLENTSDEDERKELIRETEELLIR
                     MDWLITSLLKLSRLDAGIVVFQKEQIDVNNLISSALHQLLIPMELHNITLHIDIPKGV
                     RILGDLNWLSEAIQNIFKNCMESVGDNGKIDIICEDNFLFTQLTIHDNGAGFKKEDLP
                     CLFNRFYRGKNSSTAGYGIGLALCKTIIMRQGGTITAQNHPQGGAIFVIRFPK"
     gene            complement(35822..36508)
                     /locus_tag="KR250_000862"
     CDS             complement(35822..36508)
                     /locus_tag="KR250_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0066827.1"
                     /translation="MKRIFLVEDDKAIAKNLILLLNSEGFIVTHVSTRSEALVEIAKN
                     KFDLALIDISLPDGNGFTICTEIKEKQDIPVIFLTASGDESSVVTGLNMGADDYITKP
                     FRPRELIARIRTALRKSGRFGSTFEICGLYVDTATGVVKKNGNEVFLSALEYRLLLVF
                     ISNPKSIITRVRLLDELWDAAGEFVNDNTLTVYIKRLREKIEDDPSSPQIILTVRGTG
                     YRLGGSYVSK"
     gene            complement(37008..40214)
                     /gene="carB"
                     /locus_tag="KR250_000863"
     CDS             complement(37008..40214)
                     /gene="carB"
                     /locus_tag="KR250_000863"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HBH0066828.1"
                     /translation="MPKLDSIKKTLVLGSGPIIIGQAAEFDYSGTQACQALKEEGIEV
                     VLVNSNPATIMTDKEIADKIYIEPLTIEFIEKIIEKEKPDSLLAGMGGQTGLNLAVEL
                     HDAGILDKYNVKVIGTSIESIKKGEDRDLFREVMKEINQPVIVSDIVTNLEAGLEFAN
                     KIGYPVVVRPAYTLGGTGGGIADTEEELREILSHGLQLSPVGQVLLEKSIKGWKEIEY
                     EVMRDGNGNCITVCNMENVDPVGVHTGDSIVVAPSQTLSDEEYQLLRKASIDIINAIE
                     VQGGCNVQIALNPHSLEYAIIEINPRVSRSSALASKATGYPIAKVAAKIALGYTLDEI
                     ENAVTKKTYACFEPTLDYVVVKIPKWPFDKFRHANRKLGTKMMATGEIMSIGSNFEAA
                     ILKGIRSLETGKYSLVHAPSEGRTLEELKARVVVPDDERLFDLAEMIRRGYKVEMISE
                     ITGVDKWFINKFKWIVEQEEKLKVLKIEDLDKEYLHELKKKGFSDKGISDLMKISPEK
                     LYELRSLYNIQPVYKMVDTCGGEFEALSPYYYSTYEQYDEVVVSDKRKVVVLGSGPIR
                     IGQGIEFDYCSVHCVKSLRKMGIETIIVNNNPETVSTDFDTSDKLYFEPLTEEEVLNI
                     IEKEKPEGVILQFGGQTAIKLAKFLHEKNIPILGTDFRDIDAAEDREKFDDLLERLDI
                     NRPKGKGVWTTNEGVEIAKELGYPVLVRPSYVLGGQGMEITYNEEKLIQYLDDAFDRD
                     HKNPVLIDKYLTGREIEVDAICDKEDILIPGIMEHLERAGVHSGDSTTMYPSQNISDE
                     IKEKIVEYTKKIALDLNVLGMVNIQFIEFQNELYIIEVNPRASRTVPYISKVSGVPIV
                     DLATKCMLGAKLKDLGYSTGVYKEPKLVSVKVPVFSMSKLSKVEVSLGPEMKSTGEVL
                     GVGENLEEALYKGFLAAGRHMSDERGVVLATVNNHDKDEFIEIAKDMKELGYTFVATE
                     GTAKSLRENGIEADIVNRIEEPRPNILDAIRNKQVDIVINTPTKGNDSTRDGFKMRRT
                     AIEFSTEIMTSLDTLKALVEVKKKHLNKDKLKVYNIAE"
     gene            complement(40407..41456)
                     /gene="carA"
                     /locus_tag="KR250_000864"
     CDS             complement(40407..41456)
                     /gene="carA"
                     /locus_tag="KR250_000864"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002845470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HBH0066829.1"
                     /translation="MKARLILEDGTVFIGKAFGYLEESVGEVVFNTSMIGYGEVLTDP
                     SYYGQIVTMTYPLVGNYGINLSSAESEKVQVKGFIVREKSDSPSNFRCEIDIDQYLKQ
                     NKVIGLEGIDTRALTKILRNNGTMKGIITLEDSKLEDVKHKLDKFSNTEAVRTVTRKE
                     VEHIKGNGPKVAVMDFGVKRNILRSFIARGCDITIFPATTSPEDVLSINPDLIFLSNG
                     PGDPEDLEDVIENIKALIGKKPIVGICLGHQLLALALGGKTAKLKFGHRGGNHPVKDL
                     EEGKVFITSQNHGYYVSEVPEQMKVTHINLNDNTVEGMRHEKLDVYSVQYHPEACPGP
                     KDNDYIFDKFLELVK"
     gene            complement(41713..44919)
                     /gene="carB"
                     /locus_tag="KR250_000865"
     CDS             complement(41713..44919)
                     /gene="carB"
                     /locus_tag="KR250_000865"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HBH0066830.1"
                     /translation="MPKLDSIKKTLVLGSGPIIIGQAAEFDYSGTQACQSLKEEGIEV
                     VLINSNPATIMTDKEVADKIYIEPLTIEFIEKIIEKERPDSLLAGMGGQTGLNLAVEL
                     YEKGILDKYGVKIIGTSVESIKKGEDRDIFREVMKEINQPVIVSDIVTDLQAGLDYAL
                     TIGYPVVVRPAYTLGGTGGGIADNEEELREILSHGLQLSPVGQVLIEKSIKGWKEIEY
                     EVIRDSKGNCIVVCNMENIDPVGVHTGDSIVVAPTQTLSNKECAMLKKASLDILNAVE
                     VQGGCNVQFALNPHSFEYAVIEINPRVSRSSALASKATGYPIAKVSSKIALGYTLDEI
                     ENAVTKKTYACFEPTIDYVVAKIPKWPFDKFKKANRKLGTKMMATGEIMSIGSNFEAA
                     ILKGIRSLETGKYSLVHTPSEERSIEELKKRVVVPDDERLFDLAEMIRRGYKVEMIEQ
                     ITGIDKWFINKFKWIVEQEEKLKVMKIEDLTKDYLLELKKKGFSDKGISDLMKISPEK
                     LYELRSLYNIKPAYKMVDTCAGEIDAISPYYYSTYEQYDEVVVSDKKKVIVLGSGPIR
                     IGQGIEFDYCSVHCVKSLRKMDIETIIINNNPETVSTDFDTSDKLYFEPLTEEEVLSI
                     IEKEKPEGVILQFGGQTAIKLAKFLHEKNIPILGTDFDDIDAAEDREKFDDLLERLDI
                     NRPKGKGVWSLNEGIEVANELGYPVLVRPSYVLGGQGMEITYNEEKLSQYLQDAFDRD
                     HKNPVLIDKYLTGREIEVDAICDKEDILIPGIMEHLERAGVHSGDSTTMYPSQNISDE
                     IKEKIVEYTKKIALDLNVLGMVNIQFIEFQNELYIIEVNPRASRTVPYISKVSGVPIV
                     DLATKCMLGAKLKDLGYGTGVYKEPKLVSVKVPVFSMSKLSKVEVSLGPEMKSTGEVL
                     GVGENLEEALYKGFLAAGRHMSDERGVVLATVNNHDKDEFIEIAKDMKELGYTFVATE
                     GTANSLRENGIEADIVNRVEESRPNILDAIRNKQVDIVINTPTKGNDSTRDGFKIRRT
                     AIEFSTEIMTSLDTLKALVEVKKKHLNKDELKVYNIAE"
     gene            complement(44972..46015)
                     /gene="carA"
                     /locus_tag="KR250_000866"
     CDS             complement(44972..46015)
                     /gene="carA"
                     /locus_tag="KR250_000866"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HBH0066831.1"
                     /translation="MKGKLILEDGTVFEGKAFGYLKEAIGEVVFNTSMAGYGELLTDA
                     TYYGQIVTMTYPLIGNYGINLEDVESNKVQVKGLIVREKSDNPNNFRCEMDIDTYLKQ
                     NKVIGLEGIDTRALTKIIRNNGTKRGIITLENVSLQDVQSKLEEHCNKDSVKNVTRKE
                     IENIKGTGAKVVVIDFGVKNSVLESLKACNCDITIVPAMTTVEEILNINPDLILLSNG
                     PGNPEDINDIVENIKKLIGKKPLTGIGLGHQILALALGGKTSKLVFGHRGGNHPVKCL
                     ETGKVFITSQNHGYNVSEMPENTEVTHINLNDNTIEGMRHKELPIYSVQYIPEYTTKE
                     DLDCIFNKFLALI"
     gene            complement(46041..46757)
                     /gene="pyrF"
                     /locus_tag="KR250_000867"
     CDS             complement(46041..46757)
                     /gene="pyrF"
                     /locus_tag="KR250_000867"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HBH0066832.1"
                     /translation="MINGKEKLIVAIDTDEFDKAKELIDRLEDSVDIFKVGLEQYVAT
                     KGKTIDYLKEKGKKIFLDLKFHDIPNTMKSAVKAAVRDNVWLMTIHVSDMEGMRQCAL
                     IAREEAERLNIEKPLVVGVTVLTSLSNQDLQDIGCNMTTEELAIKRAKLAKESGIDGV
                     VCSAQEVDKIVEACGTDFITVCPGIRPKSAEVGDQKRVVTPSDAIKKGAHYLVVGRPI
                     TKAENPRESAINIVREIENA"
     gene            complement(47133..47768)
                     /locus_tag="KR250_000868"
     CDS             complement(47133..47768)
                     /locus_tag="KR250_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1847 domain-containing protein"
                     /protein_id="HBH0066833.1"
                     /translation="MYKCGKCNLRGCSNGNMDKLMKICPTREIELQQKAKQLYEEEEN
                     RKIAYNAGLVEAEGYCQYTRLKEIIEFAKKCGYKKIGIAFCIGLKREMQTVQKIFEHH
                     GFEVFSVICKNGAMPKSTIGLEASQTISKCSDEVMCNPIGQALFLNEAKVDLNVILGL
                     CVGHDTLAMKYMEAPITTLVVKDRVTGHNPVAAIYNAESYFEKKLFDKDEE"
     gene            complement(47792..48706)
                     /locus_tag="KR250_000869"
     CDS             complement(47792..48706)
                     /locus_tag="KR250_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0066834.1"
                     /translation="MELYEAIFYRKSVRNFSSKKIKKPLMEEIKRSCKNLEYLNEDLN
                     IKAHVVDRGHIIHFLMGKECKVKAPHYIVVTSDKGEDYLQNIGFATEGVVLRLTTLGI
                     ATCWLEGNLKREDILEFVDIGNKNFEEDEEDDLDYLAGIKTEEDDENKIENPYIIIAF
                     GYPAKNEELFRTRYARPDRKPVKDMINKINRNRKWKDILELTRRAPSVKNSQPWYFHK
                     DERGLHLFEKRPKKHCEDMNKVSLGVALRHFDIACIKNKIDVSYEKLPIRNKIGKSYF
                     ITVVEHVKPEEETQEENVTLEKEESQDE"
     gene            49307..50536
                     /locus_tag="KR250_000870"
     CDS             49307..50536
                     /locus_tag="KR250_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBH0066835.1"
                     /translation="MNLNRNLEKYAELAIKVGVNIQPGQILLIKSPIECADFARNALK
                     EAYKCGAKNVYIEWSDEESTLIKYLYAPDEAFHEFPKWTAEQYVDIAKEGGAFLSVYA
                     QNPDLLKDVDPERVANFQKASGKALKEWRSYTLTDKCKWNIVSVPTKDWAKKVFPNLS
                     EDKAIEKLWDAIFKCSRVDGQDPIKAWEEHNENLKSKMDFLNKNNFKTLKYKSSKTDL
                     TLDLPKGHVWLSGASKDPNGISFNPNIPTEEIFGMPHKFKVNGTVYSTKPLVYGGNII
                     DNFFLTFKDGKIIDFSAEKGLDTLEKLIETDEGSHYLGEVALVPYNSPISNTDIIFYN
                     TLYDENASCHFAIGSAYKTCLEGGNNLKDEELDEHGVNDSLTHVDFMIGSADMDIIGE
                     TYDGKQIQIFKNGNWAF"
     gene            complement(50741..53122)
                     /locus_tag="KR250_000871"
     CDS             complement(50741..53122)
                     /locus_tag="KR250_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HBH0066836.1"
                     /translation="MEIRQYLKNNILIFDGAMGTMLQQKGLKLGENPEVFGLQNPDKL
                     IEIHTAYLEAGSNVILTNTFGCNELKLDSKYTVEEVIDNAVLVARKAIENVDNTKPRY
                     VALDIGPIGEMLEPMGTLSFDKAYEIFKRQVLQGVKSGVDVIVIETMMDLYEAKVAVL
                     AAKENSDLPIFCTMTFDEGGRSFTGCMPECMVATIEGLGVDAIGVNCSLGPKQLLPIV
                     EKIASRATVPVMVQANAGLPNIVDGEAIYDVDAKEFFEGVKKFVEVGATIIGGCCGTN
                     PSFIKEISENINSVTKGCIEKIDKCVVCSPSKFVEVQSPTVVGERLNPTGRKSLQEAL
                     KNENVDYAINLGLEQVNAGAQILGVNVGLPEIDEKKLMPKLIREIQAVVDTPLQVDSS
                     NVEALEQGLRYYNGRTIVNSVNGKEESLESILPIVKKYGSCVVGLTLDEKGIPKKAEE
                     RFEIAKRIVNRAVSYGIKPKDIFIDCLSLTVSAQQEEAIETIKAITMVKTLGVKTILG
                     VSNISFGLPNRKALNASFLTLALGAGLDLAIINPNEYSMMEAINSFKILNNTDKGCIN
                     YINQYSNINNSKKSDSSTKIDKDLPLDILVERGLKDEAKNVTLNLLKEKDENYILDEI
                     LIPALDKVGKRYDSGDIFLPQLIQSAETVKVSLNTIKETLLSKSSNNVSKGKIIVATV
                     KGDIHDIGKNIVKIMLENYGYEVIDLGKDVPIEEVVEVAKKRNIKLVGLSALMTTTVQ
                     SMKDTIQALRDNEINAKVFVGGAVLTEEYAEEMGADYYSKDAKSAVEIAKLNF"
     gene            complement(53094..53819)
                     /locus_tag="KR250_000872"
     CDS             complement(53094..53819)
                     /locus_tag="KR250_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Vitamin B12 dependent methionine synthase,
                     activation domain protein"
                     /protein_id="HBH0066837.1"
                     /translation="MENNLIGREDISINQSEVLRYLQYRGQEIDKDLKYTIDECVAIT
                     KNKINPRYLSRIYPIRINKDNVVELEGTNLTLESKYVYELLKECNECVIMAATIGIDI
                     EKEIRRHSYSNLTKGLIIDSCGTTAIEEVCDIVQNNLEKVFLKQDKFITMRYSPGYGD
                     LPIEKNIDILNVLNAQKQIGLTITSNGIMIPRKSVVAIIGISNTRINKSKKEKSCENC
                     KNYRNCTYRKGANSCGNKTISEK"
     gene            complement(53809..54264)
                     /locus_tag="KR250_000873"
     CDS             complement(53809..54264)
                     /locus_tag="KR250_000873"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HBH0066838.1"
                     /translation="MEKVESFKLDHTKVKAPFVRKCSVLDGVKGDKVTKFDLRFLQPN
                     VESFGTAAMHGLEHLLATYLRDTLDGVIDLSPMGCRTGFYLILWGDVDAKTVKIGLEE
                     ALKKVLESDKMPAATAIECGNYRDLSLFGAKEYAKDVLDKGFSLNIYGE"
     gene            complement(54546..55727)
                     /locus_tag="KR250_000874"
     CDS             complement(54546..55727)
                     /locus_tag="KR250_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBH0066839.1"
                     /translation="MKNKSLLNHMFRPILDLIISCALTSMLILTSMYIIKSLYVNSDM
                     SVVNQLVYQARDFRSLIGYDLFYPFLFTITSLAIYSIISYKRNKQIVDFIRKTNRKNQ
                     KLEIIKSAVNLMDEGNLEKSIDIEYDLDILKDKEKDDIDELAHKINNIVHQLRNITIE
                     ERQAQQTKTDLITNVSHDLRTPLTSIMGYLGVIEEGKYKDEVQMMYYVDIAYEKSKNL
                     NVLINDLFELTKMQNNTIKLNKIEINLVELLSQVVSQFEIYFKQEFMVSRINFNEEKL
                     IVKADPNKLVRAFENLITNAVKYGKDGHYVDIVTEKKEDMAVVKVINYGEPIPVLDLP
                     NIFDRFYRVEKSRNRNVGGSGLGLAITKNIVNLHDGEITVSSDKYQTVFEVQLPIIQS
                     A"
     gene            complement(55717..56409)
                     /locus_tag="KR250_000875"
     CDS             complement(55717..56409)
                     /locus_tag="KR250_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0066840.1"
                     /translation="MNNTVLVVDDEKEIRDLIEIYLLNSGYNVIKAEDGKEALDILKK
                     ENIHLMILDIMMPKMDGIEVCRKVRESLNIPILMLSAKSEDMDKIQGIMTGADDYLTK
                     PFNPLELTVRVKSLIRRAYYFSGANNEDKDVIKVGVVIIDKEKHSVTVAGNEIILTSR
                     EFDILYLLANNKGRVYSTEEIFEKVWKEKYYQSNNTVMVHMSRIRDKIEKYTDGEKII
                     HTVWGVGYKIEK"
     gene            complement(56543..57259)
                     /locus_tag="KR250_000876"
     CDS             complement(56543..57259)
                     /locus_tag="KR250_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBH0066841.1"
                     /translation="MKKFKLIGAIILVVFISVFISKDRLLKKDKDPIVAAQHDSEQDV
                     EANSQNKNHSNSISNILLVNKTNGISKNYTPENITKVNIPFVEEATEEEKQMAGEPAK
                     AVEDLVKQANSEGIQFLGSSAYRSYDTQLDTYTRRVKSQGREKADAYVAKPGYSEHQT
                     GLCIDLTNPERWFVGSTKEAKWLAENAHKFGFIIRYPEGKEDITGTAYEPWHIRYVGK
                     DAAEEIYSKGLTLEEYLQNK"
     gene            57384..58118
                     /locus_tag="KR250_000877"
     CDS             57384..58118
                     /locus_tag="KR250_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HBH0066842.1"
                     /translation="MSIQKLLSKARKAIQDFDMIQENDKIAVGLSGGKDSLTLLHILK
                     SYQRFSPQNFELIAITLNTGGVDNSPLDKLCKEINVPFYEFQTDIKEIVFDIRQEKNP
                     CALCANLRRGALNDNAKKLGCNKVALGHHKDDAIETFLMSMFYEGRVNCFSPKTYLDR
                     QDLTVIRPMVYIEEYMTKKISKDSNYPIITNPCPANGHTRREYIKNLIANLNKEMPDF
                     KRNVFGALNNSEKLFIWDKEKIKNFK"
     gene            complement(58403..59254)
                     /locus_tag="KR250_000878"
     CDS             complement(58403..59254)
                     /locus_tag="KR250_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBH0066843.1"
                     /translation="MNSKIKYFLKFFILIFILIFIYSVAIEPSLLIVKKYNISENGTL
                     IKSDTIESKSERIKVVQISDTQIGSFYSTKNLKKVANKINTLNPDIIVFTGDLIDYSN
                     KNPSVDEITTILSSMNARLGKFSVFGNHDYMYKLPRYYRQIMKNSNFNLLVNENKKIK
                     LKDDKYINILGVDEILNGNPNIKYLESQIDNKNFNLLLAHEPDLVDMLSKDTMNLVLS
                     GHSHGGQIRLPIKGALVTPPYGRKYTKGFYDINGNHLYVSSGLGSTKLPFRFFNIPEI
                     IEFNIII"
     gene            complement(59280..60218)
                     /locus_tag="KR250_000879"
     CDS             complement(59280..60218)
                     /locus_tag="KR250_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBH0066844.1"
                     /translation="MKSILIMGGSDFIGSALAKRLIKCGYQIDILTNGKKEIDYNGFK
                     EHLICDRKVRKDMENIITGRKYDYIYDMTAYTKEDVSNLIDFISMDNLKKYIVLSAGA
                     VYKDSGRNIKEENEKGENENWGKYGLNKKEAEDFIINSPIPYIIIRPTYIYGENNNLY
                     REYYFFEKIEKNEKIPVPKGKQVSNQFIYIGDLVKVLESIMKNPHVREAYNVTNPQLI
                     SWDDLIYTCGEIIGKEPIIKYVDMEKVEFRERTYFPFRNIDFNLDINKLIEHGLYIPN
                     VLLKEGLTATYKWFSANKPKMHDRKMNKVESVMQIV"
     gene            complement(60321..60971)
                     /locus_tag="KR250_000880"
     CDS             complement(60321..60971)
                     /locus_tag="KR250_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBH0066845.1"
                     /translation="MKKNYEIVLFDLDGTLTDPKEGITKSIQYSLNSFGIKEDLENLD
                     QFIGPPLHDTFKEYYKFEDKKTKEAVEKYREYFADKGIFENKIYENIKEILEMLYKNG
                     KILLVATSKPTVFAETILRYFDIDRYFKYIAGSNLDGTRVNKNEVIQYVLDLCNVKDK
                     DKVIMVGDRKYDIIGAKKIGIDSIGVLYGYGSFEELSESEPTYIVENVESIKDILL"
     gene            complement(61121..61309)
                     /locus_tag="KR250_000881"
     CDS             complement(61121..61309)
                     /locus_tag="KR250_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HBH0066846.1"
                     /translation="MKAFVDKDVCVACGACIGTCPDVFSMSDEGHSVAIEGEIPADLQ
                     DLCKEAEEGCPVSAITVK"
     gene            complement(61416..62600)
                     /locus_tag="KR250_000882"
     CDS             complement(61416..62600)
                     /locus_tag="KR250_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBH0066847.1"
                     /translation="MKNLLIRNGTIISPTDGIKFEKRDILVLHGKIYAIGLNLKEKIR
                     KEKLLTNKEFEIINADNMYISPGFIDVHTHCYKRKLPTGMDSDSIGIEKGSTVIFDAG
                     TAGPLNYYDFKKKYMDECKTEVYSFLNVTNEGLNILNESTSLDVINFDKVESVVEKNI
                     EKIKGIKVKASKFQSNESGIDIIKKSKQVAKKLNLPLVVYIGEYPSYIDEVMNILGKG
                     DVVTPVYGNSTNGILKESGTLRKSVINAKNRGVLFDVCHGLNQFDFKVFKKAIKQGLE
                     PDLISTDMHIKNMKDVNYSLLSVINKIMELGISLEKCIEKVTEYPTKIFDLHGNKGRI
                     KIGGEGDFTIFTMEECNDIIKDYNNNELVLNKKLRLQYTVKSWMKSSEVYRHGYENTI
                     IN"
     gene            complement(62742..63659)
                     /locus_tag="KR250_000883"
     CDS             complement(62742..63659)
                     /locus_tag="KR250_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HBH0066848.1"
                     /translation="MNKIDLNLLEKVAELRGTPLGAYNIRKNGQGVERHSTENVTIIP
                     KTDKPGIDIIVKPNTKGENVHIPVIVTETGVQDMVYNDFEIGENADVVIIAGCGIHNC
                     GCDDSEHNGIHRFFLRKGARLKYVEKHYGEGDGEGKRILNPVTIVNMDEDSYCEMETI
                     QIKGVDSTKREMDANLGKGAKIVVFEKLLTHGNQVAESNMKVILEGENSSAQVVSRTV
                     GQDNSKQKFNPCVIGDSLCSAHVQCDSIIMGNSQISAIPEIAANHPDALLVHEAAIGK
                     IAGDQILKMMTLGLTEEEAEQQILNCFLK"
     gene            complement(63681..64409)
                     /locus_tag="KR250_000884"
     CDS             complement(63681..64409)
                     /locus_tag="KR250_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBH0066849.1"
                     /translation="MLEIKNLSFNVESNNEELGIINDVSLSFERGKLIVITGPNGGGK
                     STIAKLIMGIEKATSGQIILDGEDITNLSITERAKKGIGYAFQQPPRIKGMTVENLLT
                     LAHGKPLSTDVCCQYLTDVGLCSKDYLNREVDNSLSGGEMKRIEIATLFARDLKVSIF
                     DEPEAGIDLWSFGKLNESFKKIHEESNQTIIIISHQERILELADEIIVLQDGSVKSHG
                     TKEAILPEIMCQVNSSCELMKDMN"
     gene            complement(64605..64805)
                     /locus_tag="KR250_000885"
     CDS             complement(64605..64805)
                     /locus_tag="KR250_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0066850.1"
                     /translation="MFKKMAVLKDIATKIGRKKAYELLEMVEGNDAFVAEVKIKKNGI
                     ESKKEEIMLKDNQKIILEYIEG"
     gene            complement(64930..65310)
                     /locus_tag="KR250_000886"
     CDS             complement(64930..65310)
                     /locus_tag="KR250_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBH0066851.1"
                     /translation="MINKIGKITLYVNNQEDAKLFWTEKLNFVVTYEKEMGPNMKWVE
                     VAPSKNEFTTFVLYDKNLMMRQNKEINVSHPSIILSTRDINGTYEQMANNEVEVGELM
                     EMPYGRMFSFKDQDGNDYLVREDK"
     gene            complement(65583..66941)
                     /locus_tag="KR250_000887"
     CDS             complement(65583..66941)
                     /locus_tag="KR250_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBH0066852.1"
                     /translation="MFIRKNVIKLAIPIMIEQTFVMLLGVFNTMMAGHIGEEAVSAVG
                     MVDSINNIFISVFAALSVGATVVVAQQIGKKDGEKVNETIKQALVSGVILSFTITILM
                     WVFRTGVINLFYGSAEELVKSNAKLYIEFTLFTYPFIATQQIANGLLRGCGDVKKPMY
                     VTMFMNIVNVTLGYILIYGIDLNPLGINLQTPSYGITGAAISIAIARIIGCIVIIRAL
                     FKGNEFISMGKIFPFKIDVETQKSIFNIGIPAGVEQLLFNAGKLIVQVFIVTMGTSAI
                     ASNAIGMSIASIINVIGNALALSATTLVGQYIGRGDIKGAKSTLLYLTKFATICLFIV
                     GAIFIPIAPWISSMYTQNASVIDISTQLIRSDCLAMVIWPISFVLSAGLKGAGDTRYT
                     MMTAVIGMWIFRIFTGYVLGVVLEFGVLGIWIGMYTDWLVRGTLYCLRLRGTKWLKHK
                     VA"
     gene            67389..67841
                     /locus_tag="KR250_000888"
     CDS             67389..67841
                     /locus_tag="KR250_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0066853.1"
                     /translation="MVNLNANNLNVNMVYPISSTAPIRFRRYTVSYLENMENIYLTIA
                     TYFDTLGLGTMNIDKVYGQWAWFTDNIYDLNLFVFVGNYPYQIAKNRYDTFVDILPVA
                     ITSIVNGDRIFLISNPCLLNSKITVRFISSHPTLNRTVDYGNIRDFIF"
     gene            complement(67998..68615)
                     /locus_tag="KR250_000889"
     CDS             complement(67998..68615)
                     /locus_tag="KR250_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HBH0066854.1"
                     /translation="MEIFAIGHSNYPYDKLINMIKKYDINCVVDIRETPYSKYNIQYN
                     KEAFNESLRNSGFLYIYMGKEFGAKRTNKDVYTQEGYADFEKVAKEDIFLNGIERLKK
                     GCQMGYRIVLLGAMQEPIRCHRSILVGKVLNKEGFDVKYIMHEGNLAYQEDIEESLLD
                     KYFSDRKQLSIDNLLGSALTREEMIQEGYKLANKEIGYRTEKLGK"
BASE COUNT        21621 a        12631 c         7446 g        27012 t
ORIGIN      
        1 ttttttatgt ttgccctttt cttaaggaca agtaatacta tatcatctta aaaatgtttc
       61 gtcaatactt tttttgtttt ttattttaaa aaaggttata acttttgttt tttttaataa
      121 atataattaa aactaataga tataaccctt gatattacaa acaatacatg catatataat
      181 tttacctatt ttgcaatttt attttcataa gaatatgaat tagctctcac tctatctgcc
      241 caaacattta tactcttaac tttattaaca ttagttgtac cctcaaaaat atccattgca
      301 tccttacaaa aatatatcat aacatcaaga accaaaaatc caacacaaaa taaacataaa
      361 aaattaatta cttgaaacat cagacattcc cccaataatt ttttatatat agtattctca
      421 taaattattc tttttacctt ctcataaatt attctaaata ttttcataat aaattacttt
      481 caatcatatt ttctaaatat aaagtctcaa tcaacaaaaa ctaatatcaa tttctaacgt
      541 ttgttggtaa tttataaatt aaatatctaa aagaatcttt gaagcatata aaaagcattc
      601 tacactgaca ttttaaataa aatttagcta tttattgata atttaatagc taaactttat
      661 ttaaaactta gtagaatgct aatatttctt agttttcttc ttctttattt ggattgttat
      721 caataggttt catagtcggg aataataata catctcttat agatggagag ttagttaata
      781 acataattac tctatctata cctattccta gtcctcctgt aggaggtaga cctacctcta
      841 atgcttttaa gaaatcttca tccatttcaa aagcttcatc atcacctaat tctctttttc
      901 ttaattggtc tataaatctt cctttttggt caatagggtc attaagttca gaaaatgcat
      961 tagctaactc ccacttatta gcaaatgcct caaatctatc agttcttctt gggtcttcaa
     1021 catttctctt agataatgga gatacttcaa ctggatgatg agttataaat gttggttgga
     1081 ttaatttatc ttctccaaat tcttcaaaga aagcatttat tacttctcct cttctcattc
     1141 caggttttat ctctatacct ttttctctag caacttctag tgcctcttca tctgtattta
     1201 tagtagaaaa atctacacca gaatattctt taacacaatc ttccatactc attcttttcc
     1261 aaggtggtgt aaagtttatt tctgttcctt gatagttaac tatcatactt cctgtagcaa
     1321 cttcagccat atgtgcaata acattttctg ttatttccat catatcagta tagtctgcat
     1381 atgcttgata taattctatt gcagtatatt caggattatg ttttaaatcc ataccttcat
     1441 ttctaaacat tcttcccatt tcataaactt tatcaaatcc accaacaata agtcttttta
     1501 aatacaattc attcgctatt cttaaataca ttggtatatg taaagtatta tgattagtta
     1561 taaatggtct agcatttgcc ccacctgcta tagtatttaa tattggagtc tcaacttcta
     1621 aaaatcctct atcatctaag tatgctctta atgcttttaa tgcttttgtt ctaattaaaa
     1681 aagcattttt tacttctgga tttactatta agtcaacata tctttgtcta tatcttagtt
     1741 cttggtcttt taatccatga tatttttctg gtaaaacttg taaagattta catagtaata
     1801 ctacagattt agcctttaca gatatttcac cttttttagt tttaaagatt tctccttcaa
     1861 ttccaactat atctccaata tcatatgttg aaaatacact atattcttcc tctcctatag
     1921 catctagtct tacataagat tggattcttc cctcttggtc ttgaatatct ataaagccag
     1981 ctttcccttg tattctcttg ctcattatac gtcctgctat ttttgtagtt ttaccctcaa
     2041 gagaatcaaa gttatctttt atatccatag aataatgagt tctatcatat ctactttctt
     2101 taaaaggatc tctacctgat tcttgtaatt tttttagttt atctcttctt acttgaagta
     2161 cttcactaag gtcttcttga atttgcgctt cttcattgct ttgttgatta tttttcattt
     2221 ttttcctcca aattatcttc ttatttctaa tatttcaaac ttagcaactc catcaggaac
     2281 ttgcacttcc acaacttctt tcgcagctct acctaaaagt gctttcccta caggagattc
     2341 gtttgatatt ttaccatcat atgggtctgc ttctgtcgaa ccaactatag tatattcaac
     2401 ctcttcatca aattctaaat ccttaacttt aactattgat cctattgtaa caacattaag
     2461 gtctattttg ctttcatcta tgataacagc ttttctaacc atattttcta attttaaaat
     2521 tctttcttca acttgtgctt gttccttttt agcttcatca tactcagcat tttcagataa
     2581 atcaccaaaa gatatagcaa cttttattct ctctgcaact tcttttctct ttacaacctt
     2641 taagttttct aattcttctt ctaatttatc atatccctct tgtgtcaata aaaactcttt
     2701 attttcttcc attataaata caactccttc acaaatttct taggaatttt cctaattata
     2761 ttttaaaaat tttataatct ttttttaatt tatgcgctat attataagtc actattattt
     2821 ttatgtcaag gagtcttcca tatagtactc caatttcgac acaacttctt gatagtcttc
     2881 tattttgtta atttcatccc ttaattttgc agagtttcta agtcctttta aataccaagc
     2941 gatgtgtttt ctcatttccc ttacagctac atactctcca tgttcttcta ctgcaagttt
     3001 taaatgctta atggcagtac ttattttttc gttaagagtt ggctctggca aaatctcgcc
     3061 tgtattcata taatggttta ttcttttaaa tatccaaggg ttaccttgag cacccctacc
     3121 tatcattata gcatcacaac cagttttgtc taacatattt attgaatctt ctattgtaaa
     3181 tacatcacca tttcctataa caggtatact caaatttttc ttaatctctg caattatatc
     3241 ccagtctgct ttgcctgtat aaaactgttc tctagttctt ccatgtattg ctaaagcact
     3301 tattccacaa tcctctgcta tctttgctat ttcaacagca tttacactat tatcatccca
     3361 gccttttcga atctttaaag tcactggctt ttttgagttc ttcactactg ccctcaatac
     3421 ctcttctgca agcttaggat ttttcataag tgctgaacca tctccatttt taactacttt
     3481 tggagctgga caccccatat ttatatcgag aatctcattt gaataatcat tcattatctc
     3541 tgctgctctt cccataaact ctggttctga accgaatatc tgaactgcta caggatgttc
     3601 ttcctcttct atcttaagca tttttttagt attttcatca tcatagcaaa gtgccttacc
     3661 atttatcatt tctgtatata aaagcccaca tccttgttct ttacaaatta atctaaatgg
     3721 aaggtctgtt acacctgcca taggtgatag aaaaacttta tttttcaatt ctaaatttcc
     3781 tattttcatt gtttttctcc tatttttgcc tttattttgt gatttaactt taaattctta
     3841 tttcctcatt ataatatacc acatatagct agaaatctat cataaaaagg gaattggata
     3901 tataacccaa ttccctgtat attaattatt cttggttttt ttcatatatt attctcaatc
     3961 cttctagtgt taaaaaacca tctacataat ctatactttt cgtctctgaa gcaatcagtt
     4021 tagctaaccc acctgtagct ataaccttaa catcatcaca attcaattct ttcttcatca
     4081 tacttattat tttgtcaact aaaccaacat atccataaat tattccagat tgcatggctg
     4141 atacagtact cttacaaata gtcatacctg gcttagctaa ttctactcta ggtaatttag
     4201 acgcactttg aaataacgcc tcactagata tttttattcc tggtgctatt gttccaccca
     4261 aatattcacc tttttcagag atagcacaaa atgtagttgc tgttccaaaa tcaacaagta
     4321 tacttggtgc tccatacttt tctatccctg ctacagcatt aactattcta tctgccccaa
     4381 cttgttttgg attatcatat tttatattta gacctgtttt tatgcctgga cctactatta
     4441 atggctgttt tttacagtac tttatacaaa agttttcaag agaatgcata acattcggaa
     4501 ctacagatga tattataaca tcatcaatat cacttatatt cacattatca taatcaaata
     4561 ggttacttat taggattcca tattcatcag acgttttttc tctatctgtt ttaattctcc
     4621 agtaattaac taatttgtca cctttatata tacctaaaac catattagta tttccaacat
     4681 caaatactag aagcatatgt cattaacctt ctttctattt tttgcttttc ttaagtacag
     4741 tggcaacagc agctactact aaagccccta ctattgcttc tggcacccca tttgctgcgg
     4801 caagagccat tattactttt cctgctgaac cacttttacc cattgcatga agatacttgt
     4861 cagcatataa gacatatatc attcctaaaa ctccagctgt atttaacaga gaacctattg
     4921 cccctgtaat taaacctgat atgtatacct tatttgtagc cttaattatt aactgatata
     4981 cataataagc taatacacct atcataactc ttggtaatat tgaaactaat ggatttataa
     5041 aaacaaaaga tgttactgtt gggtttgtta tagcatttaa gagacttgat atcccaaata
     5101 taaatcctat tgctgcccca actattggtc cttccataat agctccaata ataactggta
     5161 tgtgcattat agttgctttt gtaggtccaa ctggaataaa tcccaatgga gtcatagata
     5221 gcataataga aatagctgat agtactccta ttatagtcat ctttcttaca tttacttttc
     5281 ttactgatgc acttgtgttc atcataaaaa atacacctcc gttccagctc attaagatgc
     5341 cggactactt aattttttaa tttttttcgt ttttttctta taactatgag tcaagctacc
     5401 attgtaggat gcccgctcta acaagaataa ttttatcaag atgagttgcc attgtcaaca
     5461 atttgacaag aaaaatatgc taatttcatc atatttctcg tttttttact atatctttat
     5521 atgccattat attttctaca ctatccgctt tttttactgc atttattata cttttttcca
     5581 caacctctac agccaatgaa cctactaatg ttatatctgc ttctatttct cctgttgata
     5641 ttgtgaatat tgtatctcca tcatgaggtg tatggatagg aaaaatagac ttagcatatc
     5701 catcatgtcc catttgagat atctttttac atccagcttt ggttaactta gcatttgttg
     5761 caattatccc aattgtagta ttatctacac taaatccacc tttactttca ccttgtttca
     5821 ttaatttata tgtatcaatt gtttctgtct ttttatcatt taaagcacca gctataacct
     5881 taccattttc ataaacatct ccaagagcat ttacagcaat taatgcagat acaattagac
     5941 cattatctaa ttttatagaa tagctaccta ttccgccttt catagcatat tcctgacctc
     6001 ttatctttcc aacagtggct ccacaacctg ctccataatt tccttgattt aaaatatcag
     6061 tatttgcaac ttcacaagct ctataaccca tctttaaatc tggtctacat ttaaagctcc
     6121 ctactcctaa atcaaataaa actgctccta caactatagg cactttacct acacctacat
     6181 caaatccaat tcctttttct tctaagtatc tacttacacc acaagtggat tcaagaccat
     6241 atgcagaacc tccagccaaa actactgcat gaactttttg aaccatattt acagggtcta
     6301 ataaatcggt ttctctagta cctggagcag agccccttac atcaacaccg caagtagctc
     6361 cttcttcaca tattactact gtgcacccag taagaccatt tctatcctca acctgcccaa
     6421 cctttaagcc ttctatatcc aaaatattat tatacatatc ctttttctcc tcttatagaa
     6481 acttcacctg aaataatttc ttttatactt ccatcacttc tttgaactaa taaatttccc
     6541 tcttcattta tgtctaaaca tttcactagt tctctattat ctccttttaa tacatatata
     6601 tttttatcta taattgcaga ataatctcta catattttta aggttttttc tttggaatta
     6661 tttacagtat aatctaaata taatttttca aattcagtca atacattcct aacaatatct
     6721 actctggata acatataccc ttctttatac aatgatgtgg ctttgtcagc tatttcttgt
     6781 tcaaaatcca ttattttaac atttattcca attccaagaa caacataatt tgttctttca
     6841 atttcagcag ataactctgt taatatacca catatttttt ttccatttaa aataacatca
     6901 tttggccatt ttattaatgt atttgctcct agattgttta aagctttcgt tacacttgct
     6961 cctgctatta aagttataaa tggggctttg aatggttcca tatctggttt taatataata
     7021 ctcatccaga ttccttcatg aggttttgaa tcccatcttc ttcccaatcg tcctttcccc
     7081 atatcttgat gttcacttaa aatcacagtc ccatctacac tatttatagc tatcctttta
     7141 gcatgttcat ttgttgaatc tatagtgtca agatgttcaa tattttttcc aataaactct
     7201 gtatctaatt tatagtaaat attctgtggg cttaataaat cagttggatt ttccgctaat
     7261 cgataccctt ttttatttac agattctata ttatatccct cttcttttaa ggctttcata
     7321 tgtttccata tagctgcacg tgacacccct aattgttttg ataattcttc tcctgaaata
     7381 aattcagatt tattatttaa tataatctct attattttat ctctcatatt tcacctctaa
     7441 tatgacataa tttcatgact tattttcttc taataaataa ttatacatta ttaatatact
     7501 tttaatacta ttaattttaa atttcctttt taattattat aatggttgtt ttatatttta
     7561 tattgatgat ttatataatt ttacatttta tttaaataat tttaaaataa taaaaaaact
     7621 gaaagtatca attaaattaa tacatgcaaa tataatttgc ttattaattt aatattatac
     7681 tttcagtttt agtatttaat ccacatcaga taataatctt aaaatgacca aggtcttaaa
     7741 actatttatc tttatcagat ttatcttcta aatttttatt tatatcaata acattgtttc
     7801 tttcttcttc atctttttct aattttatag ttaattgttc ttcaacttct ttagaagaat
     7861 tttcctctgt aactgcattt tcaatttcat tcagaggtaa ctctttatta aatgctttta
     7921 caaattgttc tgcatctaaa gtttcatata taagtaaagc ttgtgctaca tactctaatc
     7981 tatccatatt ttcttgtaat atacttcttg tcttatcata tgcaccatca actatacgct
     8041 ttgtttcttg gtctatctca aaagcaactt cttcagaata atttctctta cttgaaaaac
     8101 tattacctag gaatacttca tcatcactgt caaatgacat tggaccaagt ttagaactca
     8161 ttccatactt tgttaccata cttcttgcag tagctgtaac tctttctaag tcatttgaag
     8221 cacctgtaga tacatcctta agtactagct cttctgctac tctaccacca agtaaaacta
     8281 caatattttc tttcatttca ttttttgtag catagaactt atcttcaaca ggaagttgca
     8341 ttgtaaaccc tccagctctt cctcttggaa ctattgtaac ttgatgaaca ggacttactt
     8401 cttcaagtac atgggcacaa actgcatgcc ctccttcatg gtatgctgta agtcttcttt
     8461 ctttctcact tattactttc gattttttag ccacacctgc tataaccttt gttatagctt
     8521 cttctatagt ttccatctta attttctttt ctctttttct agctgttaat attgcagcct
     8581 cattcattaa gttctctata tcagcaggag taaatcctgg tgttcttcta gccaagacat
     8641 ccatttttac atcatcactt aatggtttat ttcttgagtg aactttaaat atagcctctc
     8701 taccttttac atctggtgta ccaacaacaa cttgtctgtc aaatcttcct ggtctaagta
     8761 aagctgggtc aagtatatct ggtctatttg tagctgccat gattattata ccttgattta
     8821 ccccaaatcc atccatttca actagaagtt gattaagagt ttgctctctt tcatcatgac
     8881 ctccaccaag acctgcacct ctttttctac caactgcatc aatttcatct atgaatatga
     8941 tagctggagc acttttctta gcctgttcaa ataaatctct tactcttgaa gcaccaaccc
     9001 caacaaacat ctcaacgaaa tcagaaccac ttatactgaa aaagggaact cctgcttctc
     9061 ctgcaactgc tcttgataga tatgttttac cagttccagg aggtcctacc ataagcattc
     9121 cttttggtat tcttgcaccc aattcaatat atttcttagg attctttaag aaatctacta
     9181 cctcctgcaa atcttctttt tcctcatcta gtccagctac atctttaaat gtaactctag
     9241 ttttttcatc atctttatga accttagctt ttgatttgcc aaaactcata acctttccgc
     9301 ctccaccttg agactgattc ataaacacga accaaataat caccatgaag aatattaaca
     9361 gtaatgtagg caacatttcg acaaaccaag gtgtagatgg cttagcttca ccaccaaaag
     9421 ttagtttccc agccttagct tggtctaaca cctcatctgc aagtttattt cccatcactt
     9481 ctgttggaac atatgactta aactttgtat ttgtatcttt tatagtacct tctacagaag
     9541 tttgatttac aaagtataat cttgatatat tctcatctgt taattctcta tatacttcag
     9601 agaattttaa atcttttatt ttttctgtag gcttacctga gaattgaact atacctacta
     9661 taattataaa aacaagtaga taaaaacctg ccccttttag caacttattc aatatagaag
     9721 ccctcctttc atacattgct ttgccctata atttgttaaa attaattttt aatacttctt
     9781 ttgtgttttc atcaatttta taattttcac tgattttata atctccaaca catattattc
     9841 cttcactatc tgtgattata ggaattttac atctttcttc tcttgggatt tttaaatcta
     9901 tgaataaatc tttcactttt ttacttccca tagcaagctt tatcttatca ccttgccttc
     9961 tgcttcttat tactataccc ccttttacct tattaaaatc aaacccttta gatgaattgt
    10021 ctaatttcat actcttatac ctatctatgc tcattacttg ggtctcaata atttgtttta
    10081 attcttttat tttaataaac ccattactag gtacattgta ataaaattca atttcctcat
    10141 taacaatttc ctcattcgtt aatattatac tgtctttttt tctataaaca aatatatttc
    10201 ttggaagagt taacatttta tttacttttg aattatcttc taaagacatt atatcttcta
    10261 tatgtctttg gtcaacaaaa ttagtatctc ctagtatatg ttttatggaa tttctaagaa
    10321 ctctaacttt gatagcatca tgtaagcaaa caaaatcgtc taaatttatc tctacaaagc
    10381 ctttttcttt tatatttgaa acttctctaa atttagcttc tgcctctttc tcaatataat
    10441 cgttatcact ttttaaacta ttgctcatcc tcactataga ttctattaca tttgaattaa
    10501 aattatcctt catatatggt aaaaggtcta accttatctt atttctagtg tatatatttt
    10561 ctaaatttgt tttatctatc ctcggattta aattataagc ttcacaatac tcctctatct
    10621 catttctttc cacgtctaat atgggtctaa ttatgcaatt atctctaata taatcaattc
    10681 cctttaagcc ttttaaacct gttcctctca tgatacgcat cataaccgtt tcggcttggt
    10741 cattgagatt atgacctatt gctattttat tggctttaat cttatcctta atttcataaa
    10801 acatttcata tcttagcttc cttgctcctt cttctaatga taatccctca ttttcacaat
    10861 attttggtac attaatagac tttacaaaga atataattcc catatcctca caaagtttag
    10921 atacatataa tgcatctttt tgagcttcta tccctcttat ttgatggttt aaatgtgctg
    10981 catatatttc tatattaaaa tcttttttta atctatttag tacatgcaat aaacaaactg
    11041 aatcaggtcc tccagaaaga cctaaaacta ttttatcgcc tttttgtatt aaattatgtt
    11101 tatttatagt acttagtacc ttatcaaata tcatatttat ccctcttaca cttagtttta
    11161 ttcactttta agtatacttt ctataaaaaa acgctaaacc cttgtatcaa ttttaagaaa
    11221 ttaaagttta atataaatat aataatacca aataaattaa ataaaaggta gttttttcac
    11281 tacctttata aaatttctgg aaaatagcca tagttattat ttagtaccta tgtatatatc
    11341 tattttattc ctacctaaaa ataaatcttg tacctttata ttatagtcta ttttcaattt
    11401 tatgtgttcc ccttcttgta tatttatatc taattctctt gtatcaatat caattaataa
    11461 agtaccttgt ggggtcacat actttgtagt actactttct ccacatttaa acagcattgt
    11521 tgagttaata gcaccaaact ttttgatact aacttgattt ttaagtatct ttatagtatt
    11581 agttatttct atatcttctt ctttttcctt gtaaataaca tatattccat catttttata
    11641 aaaaatttta ccaaaggcag tcatctctat ggtgtccata tttccttttt catcatattg
    11701 tctagtattt atacttattt ttacactcaa cgatttttcc tccatagatt cttcatataa
    11761 actagtattt ttgtatttct actttttgaa tcttatcaat ttttttcttt aagaactctc
    11821 ttgctatatc tgaaaatttt tcaggtatat ctgatacata atattgataa gttccatgaa
    11881 tttctgtatt gtttataata ttttgcacct ctaaaatctt ttttaaatcc tttgcagtct
    11941 ctttagctgg attaacaagt tttatatgct ctccaacttc ttcacctatt gttctcttta
    12001 aaataggata atgagtacat cctaacacta aagaatctat atttttttct ttcaattctt
    12061 gtaaatatat ctttgctgtc agtttagcca cttctgtatt tgcccatcct tcttcaacaa
    12121 taggcacaaa taaaggacat gctttattaa caatttctat actctcatct attttagata
    12181 tttccaagtt gtatgctttt gaacttatag ttccttcagt tcctattatt cctacaattt
    12241 tgttttttgt agaacttaca gctgttctag ctcctgcttc tataactcca attatcggta
    12301 tatcatattt ttcttgagct tcttttaagc ttcttgcagt agcagtgtta catgctataa
    12361 ctatagcttt tacacctttt gtttttaaaa agtttatagc ttgaaaagta tatttaatta
    12421 tagtttcttt ggatcttgaa ccatatggta ttctggcagt atctccaaag tatattatat
    12481 cctcatttgg taatatcttc attatctctt ttagaactgt taatccacct aatcccgaat
    12541 caaaaactcc tattggttta ttgttcatct ttatacaaca cctactttac atttttgtgt
    12601 ttaacaacaa cttatttatc gctaaaattt atatttatat tcataattaa tatattatta
    12661 cctaaatgat tattatagtt tattttacac aaaaaataaa ggagctatct taaattagaa
    12721 aataatttct acaaaaagac aactcctttc actacttaaa aatttaaatt tattttgcaa
    12781 acacatgatt tcctattctc atataaggat tcaatctatt tatccatttg caagtggcta
    12841 tttctggatt ccaaaaatat actgctttat tagttgggtc agttccataa agagcttcct
    12901 gagctgcttt acgtgctgag tctgttggtg ccatctttat tgaaccatca agcacaactg
    12961 agaaagcatt tttttgatat atgacttctt ctattgtgtc tggaaatcta gggtccaata
    13021 ctctatttat tactacagct gctacagcaa cctgtccctc atagctttct cctctagctt
    13081 ctcctgcaac caacttagat aataacatca agtcattatc agataaattt attacttctt
    13141 tctttgctat atttttttct tgtttgttta ctttcttaat attttctgtt ggttcatatg
    13201 caaatacctc tttaataggc ataaatataa aagtcaaagc taatagtgta gccatcagtt
    13261 tactccacct gtccatgatt aaaaccactc cttacattct tatttttaaa attttttctc
    13321 taaatccttg actacttcaa gtactttaaa tttaattttt actctacttg ttttttttgg
    13381 tgtatcttgt tttattaact tatattcttt atcagttaaa acttccttca acttatcatc
    13441 tgtagattta tctatcactc cattagattc atcaacaaaa catagtggag ggaacataac
    13501 acaccaccag ttttttcctt cacctttccc aatgataatc tttaatgctt tatattcacc
    13561 agctggaaga actatattag aatattgttt agtagggaaa ttgctatatt gtatacctac
    13621 ctttacactg taattatatc catttttaag aataatattt ttacttattt cttccaaact
    13681 actatattcc ttttttataa tagcttcact ttcttttata ctttttgaat tttgaagctt
    13741 tggctgtaaa tattttatta cttcatctct aacttttagt ttaagttctt ggtcttcatc
    13801 tgtattgctg tttgctataa catgaaatct tattaattta tctttataat cttttgatat
    13861 attgtctact ttttttacct caccatttat tactattgtc attattgata tgactgatat
    13921 aagacttaat attaatagtc ccaatctaat cttaattttt ctcattgaaa attccccctc
    13981 aagataaatt tatatcatca attatgtcca attaaattta atttattcat aatatttttt
    14041 aatactaata aaaaaagcta tcttaaaatg attacaatca ttttaaaata gctttttaaa
    14101 ccttatattt tataagtctt tactaatcat agccttatct atcataaact gctctgcttt
    14161 ttctatatgc tctgtagcat acttcttgga tagctcacta tcacctttaa ttatagcatc
    14221 aagtaaatat ttatgctctt caacaatatt atgtgatgta tcataagcag acatataggt
    14281 aactctaaat ctatatactt gttcccacaa tgagtttatt aattggatta attttttatt
    14341 attagttgct ttaaatatac attcatgaaa ttctacatca agttttaata acgcatctac
    14401 atcggattcc aaagtgcttt ttttaaattc ttctgatata tctttaagct ttttaatatc
    14461 ttcatcacta attctttcag cggcaagata tgctgctaag ccttctaggc ttgctcttac
    14521 ttctaataca tccataatat cctttaatga catatttgcc acatacgcac cttttctagg
    14581 caacataacg actaaacctt ctaattcaag ctttcttatt gcctctctca ctggagtcct
    14641 acttactcct aattgttctg ctagttgcac ttccattagc ctttgaccag gctttaattt
    14701 tccttctaag attgcttctc ttaaattctc aaagactaca tctcttaatg gcttgtaatt
    14761 atccaagttc agtttagtta aattctccac aaatttcaac ccctttttga ctgctattaa
    14821 caacatacac ctgtttatat ttttttaata attctttttt tccaattaga gcatcttctt
    14881 tatttttaaa tagtccaaaa actgtaggac cacttccact catcatagaa cctaaagcat
    14941 tatttttcat cattacttgt tttatatcac ttatttcttt gtgtttacct atagtaacat
    15001 tttcaagaat atttaccatg ctttctgata cagcttttat atcttcagat tttaaacact
    15061 ctatcaaata tttattattt ggtctttttt taatattatt caaatctaat ccttgataaa
    15121 cttctttagt agaaacgaat aagtctggtt tgcatatcaa tatatttgta tcacatggta
    15181 aacctttgat gtttgttagc ttttctccaa taccttgagc taaggcaggt cttcctgata
    15241 tacaaaaagg aacatctgcg cccaaattaa gtcctatttc ttttaattca tcttcagata
    15301 atctaagttc ccataggtga tttaaaccta ctaatactgc ggctgcatta gaacttcctc
    15361 cagccatccc tgctgctaca ggaatatttt tttctatgaa tatttctaca ccttttttta
    15421 tataaaattt atttttaagt attttggcag ctttataaac aatattatct tcatctaaag
    15481 gaatatctaa actagtgctt tttaccttta tttcatcttc gtctaattct tttatcttta
    15541 ctatatcgta taaatctatc gtttgcatta tcatttcaac aaaatgataa ccgtcttgcc
    15601 tttttcccaa gacatcaatg gataaattta tttttgctct actctttagt cttataaaat
    15661 ccattctatc ctccttgttt aaagaataat gcatcactaa caataatatt atactacaaa
    15721 aaataaaata atagggtatt ataataccct attatactta attactttaa attttttaat
    15781 attaatctac taatacgact tttttttcta aaataagaca cttaccaggc ttaattggtt
    15841 catcaagttt tatatcatta agttctgcaa tttcattttc tgttgtattg tatttttttg
    15901 ctatattcca gaaagtatct ccttctttgc atatatatac gattatgcta ggagctttag
    15961 ataaatcata tacaccttgg tcttctccct tgataataaa attttctgct ttcttatcta
    16021 atgcttcagt aaatctctta attttgataa tcaagtctat ctggtcccta ttcaaatcaa
    16081 cttcgacttt atcaatacat gctgtattaa acacagatgc tgtatcagtt agattatcca
    16141 tttctatatc atgttcaaat ggaatttcct cactaatttt ataaaccaat tttaaacctt
    16201 caactgggac aaataaaatt tcaactttta tgattccttg tataatgctt ttatttcctt
    16261 caatataact attttctata gacatggttg gacaaacgct tacaatatct tttatttgta
    16321 tatcatcatt atcatttctt atgccttctc taaccataaa tgtttcttca ctacgtctta
    16381 aagtcttatt taattgaata ggtttgtggt caaattttat tatcttttga ggcgaatatg
    16441 catcttgtaa tacttctctt gtaacttcat ctgtaacttt taccttacaa caagccatac
    16501 aatctatttc taaaagacct gtgttgcttt cactattttg cttgaaaata tgattaaaat
    16561 ctgacataga caacaatact tcttcagtca taccatcact aactccaggg acttctacaa
    16621 attgagtaaa ctcaatacct actctatcca actctacaag ttcaccctca tatgtacaag
    16681 ctaacggatt tatttctaat actccaccaa taataacttt attgtcagtc actctacttt
    16741 cttttacttt aacacatgga tttaaactta ttatagattg aatttcctct gtgtttattg
    16801 ttatagtatc tcttatagaa ctttctgctt tttctattcc aactatatct tgaaaacaaa
    16861 tttcctttct gtgcttttgt atgccttcca cttgcgccac atcttttaca atgtctaacc
    16921 tttgcttttc aaacaaactt ccccttatat tcataagagc tcctacttta atctttctct
    16981 cattcattat agtacaatcc atatgctcta cttctgaaaa taacatgtat tccatatctt
    17041 gaacaacatt gtctttttct attacttcgt ttatatctac ttttccgtct acattagaaa
    17101 tagtattttt atcatctgca atatagatta cattataatt aaaactacct ctgcaaagta
    17161 tcttcccatc tgccatttca atttttttaa gtgatatgta tccttctgtt tttacaattt
    17221 catatacatc taattttttg tctggtacaa ctgcctccgc ttcaatgaat gtttgaaatt
    17281 ttccaaagtc tattctattg tcaactttaa ttacatcttt aattaattcc atagtctacc
    17341 tccttaagta ttataaaaat atcacactat atttatattt aaggagcaaa aaaaaaatta
    17401 cacctatttt taaataggta taattttaac ttatttgtaa tttatcttga tctctaaaaa
    17461 cttgcaactt tacattagat gttaataaat ctgagtaact ataagatact ctaggatatc
    17521 cattactttc atcatctaac tttataacaa acacgcttgg ataaactttt tctagtatac
    17581 cctcttttgt aataatctgc tttcttcctt tattagcttt caacaatatt ttctttccta
    17641 tatgtctctc taagcttact cttatcttat ctagagtttg aacagtagcc acaatatcac
    17701 cttctttaac gtttatatgt ttacataata tcacatataa acatttttgt caaatgttta
    17761 aactaattat tttatatatt ttttgcaaaa atgtcaatac tatttttgaa gatatacagt
    17821 ataattatgt taatttttgc taatgtaata ttgtttttgg cattgcccat ctaaattttc
    17881 ctctggtttc agaaccacca attcccttaa cacctgtaat agtctgctct ataacctcat
    17941 caataggaac tactacatca attcttgaac aagcaatttt ctccactata aaaacagggt
    18001 ctaatgcatg tatcattatc cttccaggcg aacttgcata gtttgctcct gcacttatta
    18061 ttttttcata atttgattgg catgctcctg cgaatatgac aagattatcc aaatttggtt
    18121 gccatttacg tgcttctttt actgttttaa caaaatttga agagtttcta tagttactca
    18181 tatcttctat atctcctctt tttattgtca ttgcatcatg acctgttatt actagtatat
    18241 ccggattata tttttctagt agtgctctta cttctttata ctgattttgc tctgaaacag
    18301 ctacacctac tgcaggtatt ccaagttgag catatacatc caagcaaatt tttaaatatt
    18361 ctttatctcc atctatctgt agtacttttc caggcattcc atatgtgtta ggatttaatt
    18421 gaagttttgg aactgctcta gtcattcttt tttgtctttc tttagctttt cttactgact
    18481 tatataataa attttctaca tttttatcta ttaatatatc ttttatatca ggtgctttta
    18541 ctaattctag gtcatcaata taagcatctg ctattactct aaatgcaatt ccctttagta
    18601 tagctatttt ctcattattt tcatctacac caaaagagac tattttaaat actatatctt
    18661 tgttatgtga ctttctagct acaatgtctc ctaccttcat agttaaacct ccaataaatt
    18721 tttatcttag tttatactat gttcgatata aaactttgtt tcctaatgtc aaaaaaacat
    18781 ttcttataat acttctttta tcactcttaa agcattttta tagtatatct tttctatatc
    18841 atcttcacta aacccttctt tttttagagg ttcatacagt ttacccatct gagatatatc
    18901 ttcaatctca acttttgaat ctattccatc aaaatctgac cctagagata caacatctat
    18961 tcctccaaca tttacaatat gttttatgtg tctaaccata tcttcaagtt tactttcact
    19021 tttatcactt aaaaataagt ggaagaagtt tatacctgta actccacctt tatttgctaa
    19081 tactttaatc atatcatcag ttaaatttct agaatgattc atcattgctc ttgaattaga
    19141 atgtgtagca attattggtt tagaagaaat ctttgcaatt tcataaaatc caccatctga
    19201 aatatgagaa acatcaacca acattcccaa ttcattcatc ttatgtacca cttctcttcc
    19261 aaaatcagta agcccttttt ctctatattc tactttatta tgaggaaagc tcaattcatt
    19321 cacatgattc caacttattg tcatcattct aacccctaag tcgtaaaatt tctttaaatt
    19381 ttctaattta ccttcaagta ctgcaccttc ctcaatagat aaaagagctg taagtttacc
    19441 ttctgattgg tttttcataa tttcttcata gttagtagct aaagctattt catcactatt
    19501 ttttttcatt tcctcatgaa atttatttgc catactcatg caataatcaa aaggatgttt
    19561 tacttcttca gtatcaacaa aaagagcaaa tgtttgagct aatgaatccc cctttttcaa
    19621 tctgtctata tcaacagaat atttattaga cttaagttca cttgtttcta cattttccat
    19681 taattttgct atagtatcac aatgtaaatc aataaatttc atatttatac cccctgtata
    19741 ttttatattt atatctaaaa atagaaatag ttgtccttat agacaactat tgacaactat
    19801 tttttaatat tctttttaat attacttaat cttattcttc ttttaagtta ggtattactt
    19861 ttttaacttc ttcaatttga tgccatgttt tatctatatt attttcacta atatattttt
    19921 ttaaatcagt taaagatgta aaatctttaa ctgataaatt gtcatcatca tctgcaaatc
    19981 ttaaaataaa catgagactc ccttactttc cacaacattt cttatatttt tttccgcttc
    20041 cacatgggca tgaatcattt cttcctattt ttgcttcttt aactattgtt ttagacttct
    20101 tataagcctt tattatttct tttctttggt ctgctggtag tatatcttcc caaccttgta
    20161 aattatatag ccaatgagcc tcaacagtta ccatattgtt atataatttt tcccaatcta
    20221 tttttataga aacttcttta tcagcttcta cttcttctaa aactatatct tcttttaagc
    20281 tttcacttat tccatctatg aatcccatga aaaattcatt agtagttcca aatttttcag
    20341 ccaattcact tactttacct tcaacaacat ccattttatt atttaatatt tcattgtata
    20401 ttccagcttc aactttcaaa tattcttccc agaattttac ttctgcttct tgactttcat
    20461 gattatcact taaatttctc cattcagtat ataaactcat actttttcct cctaagtata
    20521 cctatatttt tatagttttt atagtataaa atcttttagt tagattggaa gtttcttctt
    20581 tttgattata ccatattcta tttaaagtta actcttatat tatatcataa ttaaacaatt
    20641 cttttaatac ttttatagca tactcttggt ttataaaata agggcttgtc gcaattgtta
    20701 ttactttggc ttttttaatt aagtttttta tcttgtaaaa tttgagttca aatggtatat
    20761 agtccatatc acttgaaaat atatccaaat ctatgtcaag aacaaactct gattctactt
    20821 ctaaatcaaa tccatataga ctatctatta ttattaattc tgaaaatatg ttatatttta
    20881 aagcaggttt tataaaactt cccacattta aaacttcatt tgtatatcta aatacatctt
    20941 ttagattatt tacatctaca tcatagttct caggttctct agtatcctta tgttggtcta
    21001 catgcactaa cttgcatcct tttgtaaact caccctttaa caaagacttt gcccaaaaat
    21061 aaaatgcatg gttatgattg tcaaacacat atatcttttt atttcccagg acatattcga
    21121 ccatattctc caatcctttt gcttttattt ccttattttc atctacttca ttaaatacta
    21181 cctcattgcc taaccttaca tcatttaatg taccatttat aagttttgga acgtatatgc
    21241 tcttattttc tcttttatca tatgaaaata cattatttcc tactggtttt tctatataaa
    21301 atccactata ttcataattt tccataaaac tttatttcct ccattgttat ataaatatat
    21361 aagttataat tttaaaaatt aaaatgcact tattgtaaat catatcatct attatagaaa
    21421 acatcttata taactaaata taatatcttc tctcaatatg tattatacaa taagtgtaaa
    21481 atatatttat ataaaatata ctacattcct ataacaaaac catcagcttc aattggaaga
    21541 taactaactc ttctattcaa ctccaccttt ccaaaaaata ctacccaata tggtctaaaa
    21601 atactttcaa ttttaactat ttcaatctct gggattcctc ccataaacct atgagttaat
    21661 ctcatagcca ttttatttgc ctttgctttt actcttcgtt cgtccttatc actatactgc
    21721 acaatattac aatccacatt ttttacaact tcatatgtag gaaaatcttg tactatacta
    21781 ccagaacctg tagaaccatt cactaaaatt tttaattcat ttatatcatt agaatatctc
    21841 ctattcttga aaatggattt cgttttgtga attttaagtg tgataatttt aaactctata
    21901 taatgaagtc taacttcatg agcatatttg ttctttaata ttagcttact aaataaagaa
    21961 ttttttccac ttttagctat ctctatagct tcttcttttg taattcttat aggtaacgct
    22021 cttatatcca taaaatcatt ctctttctta tttttttctt gtcattttta aaactctata
    22081 tatataatca aaaaatatta caattacaac tgacataact ccatatggaa ttatttttcc
    22141 aactataata tcatttctag tcaatatacc ataaaatata aatcctgtta ttaaaaagta
    22201 caataatact agaaaaccgt ctattccaag ccattttatt tctaaatctt tttttacctt
    22261 agacactaat ttagacatag cagtttctcc ttttatagat tattttgtgt atttaaattt
    22321 tcacctattc tattaccaat catccatgca ataaatgaaa aaggtatagc tatcacaaga
    22381 ggctctaact caaagctctg aactggaaat attttaaaat aaccaaaagt cactccacca
    22441 acaatcattc ctaaaatacc acccatagat gttcttcttc catttccaag aagcccacct
    22501 actataggta caaatgccga acccatccaa atacttccta caaatataca tgcatcatat
    22561 acattttcaa atctaaatgc tacgctaagc cccataactg ctgctaaaca tacacctatt
    22621 ctagtaaact ttactaattg tttctgagat aactcttttt ttgtaattct tccatatata
    22681 tcatttgcta atgtagcccc agctgctaga taatagctat ccaatgttga tattatagaa
    22741 cctaaaattc ctattataaa tattactctt attccttgtg gtaatgtatc taaaactaaa
    22801 gttatatatc cagcagctgg agccatttct ggataagtag ctcttactat aagtcctgtc
    22861 atagttaaca ccatgtcaaa acatatccat agtgaaaaac aggttaataa ggctcttctt
    22921 ccaaccttag gagaatctgc tgctgaaaat ctttgataaa aagttgggtc tgaataagct
    22981 ccaaaagaat atacaattaa catcaatgct ttcatagctg acataccctt tgtagggtgc
    23041 ataagctgtg gtgcatcttt gagtgcttct gtaagacctt gaaaccctcc tatttgttca
    23101 aattgacctg gcataaccat agttaaacca aataccatac aaaaaaacaa taccatatct
    23161 gtaatggcaa ctcccataag cccaccaagt actgtaagta caacgactac tcctaccatt
    23221 attaatccta ttaattcttt atctattttc catgctacgt tacctatagt tcccatagca
    23281 gttatttctc caagttgtgc acaatatata aacatcaaaa ttgccccaat tatagcaaca
    23341 tgcttgccat aaaaactttc cagtaaatca ggaactgtta tattggttct tatatgaatc
    23401 cttggaccaa cccacaatgc taaaggcatt ctcccaatat gtgcagctat tgaccaaact
    23461 gcccatgcag atattccata tatagccgca tactctacac tacctactgt accaactcct
    23521 ccataccatg atgcaaccaa agttcctact attaaggacc atggtaaact tcgatttcct
    23581 aggtaataac tttccatatc ttttacattt ctagaaaaat aataacctat tcctaataat
    23641 ataaatgcaa atataactat aatcccattt tcccaaaatg ttaacatatc taatctccac
    23701 attcttatat tttaattttt aactttaata tctaaatatg aacaactcta cccatttcca
    23761 taaaaatagg tagagttata taataattat aagtatttaa gtaatcttgt tattaagcaa
    23821 ttttattgtt taaaccttta ttttctcttt tatttatttt atttaaagca taagtagcaa
    23881 atatccctat aactgcacat actacagata aaataaatat atatgtatat cctgtttgtc
    23941 catatttatc taaccaatta cctgcaatag tgtaataaaa cgcatctgga gaaaatccta
    24001 taaatgaagc aaatccaact actgctcctg tagtttctaa agatatatta gactctgaaa
    24061 cagatgcaaa gtatatacct ctaaatccaa ataaaattac acttagtatt atcatgttta
    24121 ttacagctat ccatataaat cctgtactct taggaataac caagtaagca gctgtactta
    24181 ctgtcattaa tataaatcct acaaacatta ctcttataga tgaaccaact ttatctgtta
    24241 taacaccagc aactggagat gctcccattt ttataacata agttcttatt attgataaag
    24301 cagaaactag agtcattggc attacataaa cttctgataa gtatggactt aaataagtta
    24361 aacttgagaa tactaaataa gttgaaaata ttataagacc aattagccaa gcctttggca
    24421 ttgtaactac ttttaacaat gacttaatac ttaatccttc atctgatgct ccactatctt
    24481 ttttctcttc cactaagaaa aatagaagta taccacatat tatagatgct gcagaatata
    24541 accatactac atatttgaat cctactgtta tttcagcaaa tttaccaaag aagtaaagac
    24601 ctataaaaga aattaatgta cctgatacac ctgataaccc ttcataaaaa ccaaatattc
    24661 ttccttgctc tgactcactt ccttgcattc taacaacctt aacagaggct gagaagaaag
    24721 ttaatattgt tgtaaatccc cataatacaa atattaaaag tatcatacta tatgatggga
    24781 atgtagagaa ataaaatcca agtattcctg aagcaattaa agaaaaagtt attaattttc
    24841 taactgagaa tctatctgca aaccatcctc ctataaaata agataccatt cctactaatc
    24901 catataaact caataagttc cctatttgtg tatgacttaa tgctaaagct tgttgcattg
    24961 ggtcataaaa tgatgatttc atatatggta aactatacat agctgttgtt ccaaatgcca
    25021 atatgaatat tatcaaaaat cttttaacat tatttttgct ttccattgat tctctctcct
    25081 taaaatttta ttgtttataa tctcattcat ataattttat tttaattttt ctattgcttg
    25141 tttaaaatca tttattatat catttgaaga ttctaatcct acagctattc ttattagatt
    25201 ttccccattc atttgaacta atgtatgctc ttcccctaag cttgttgcta ttgttgcaag
    25261 ctctaaagaa ttcattaatc ttctactagc ttggtctctt gtcaatccat tgataccatc
    25321 cttaagttta aatgaaacta cacctccacc taaaccattc atttgagttt tacaaagctc
    25381 atattgagga tgagatttta atgctggata ctttaccaat tccacattct cttcttcctc
    25441 taaaaattct gctactgcta atccattttc acaatgcttt ttcatcctca tatctaacgt
    25501 tttcataccc cttaaaatca tccatgcact cattggtggt aaagatgctc ctgtaaaaca
    25561 acataaactt ggccatctaa ctaaatctat taattctttt gaacctgcta ctgctccccc
    25621 taaagtatcc ccatgtccat ttataaactt agtaagacta tgtattacta aatctgcacc
    25681 taaatctatt ggtctttgta caattggtgt cccaaaagta ctatcaacta tagctaagct
    25741 tttattttca tgagcaattt ctgctattgt ttttatatct actaagtcta atgatggatt
    25801 taaaggactt tctatataaa ctattttagt atttggtctc attgcttctt ttacattttg
    25861 tggatttact gcatctacaa atgtaacttc aactccaaat ttagataaca actcagacat
    25921 gaataattta gtgtgtgaaa atattccatc tgaactaact atatggtcac cactttttac
    25981 caatgctaat atagtagatg ttattgcccc catccctgat gtactagata tggcactttc
    26041 tgcattttca agcatagcca acttagcttc taactctgta agagtaggat tacactctct
    26101 tgtatacaca tatccccatg tcatacacaa ctcttcaaaa tgctctactg tctttgcagc
    26161 aaatgttgct gtttgacata ttggtgtagc caatgcacct gttgttgggt ctgcactatg
    26221 tccccaatgt attatttttg tctctaaatc ttgattaata ttgttactca ttttgatttc
    26281 ctcccatttt gacttattta tacaacgttt tccctaccaa atttcatcaa tttagcttag
    26341 tcttaataca ttttttcaag tatacttttt tatttgttat aatttctaaa aatatgacac
    26401 ttattttgtt tttttacagt tctaattata aactctatag taactataga gtcaatatgt
    26461 ttttgatatt ttttttacaa tgtttaaaat agtcagtaca aaaataggta tatcttagaa
    26521 taactaaagt catcctaaga tatacctatt tttatatcac aatataaata attacatata
    26581 agcattagtt tttttatcat aaatagattt ttcaagattt atcatctttt cataaatata
    26641 ttatagttaa atattttaat attacctaac atctacaata aaatatactc ttaaaataaa
    26701 attatataat taaaaatact ctataaaagc taaaaagttt ttctattcgc tacatctaaa
    26761 aataattaga tagaggtata aatgtattta gtttatttat acctctagtc ttttataaat
    26821 tatatctcta ctaaattgat tttttagcac gaataagtgt gctcttggag ataccagtca
    26881 tcttcacaac ctctctatat ggcttagaat ttaataaccc caatgcatat tgtatctgtt
    26941 gattgctgta tttctttggc ctaccatctt taaagtcctt tttcttttta gcaaattctt
    27001 ttcctaattg agtacgttct attatcatat ttctctcaag ttctgcaact gcaagtaatg
    27061 tagtcaaaaa gaatttaccc attgaagtat tttctaaaag gcctatattt aaaatatgaa
    27121 cacttacttc tttagcaaat aaaccttcta ttagttgtat tccttctgat gtacttctag
    27181 ctagtctatc cagcttagtt gcaacaagta agtctccctt ttttagagat gatataattt
    27241 tttcaaattt aggcctgttt gtagtagtgc ctgtataagt ttcctttatt attttagcat
    27301 cattatattt attcaaaatt tcttgctgtt gctgatacaa actgttgttg tctgtttgat
    27361 tttttgtact tacacggcaa tatccatata taataatcat cacttcctaa cataaagatt
    27421 ttgtacagaa gttacccaga agcttgtttt tacttatctt tatccatttt actaaaacga
    27481 tttttgggtg tcaatactgt taacttttga caccataata attcctttat aatttgtttg
    27541 actatgtcaa aaattaccaa tataatgaaa ttaatgagtt tatacaaatt ggaggaaata
    27601 atatgaatag tctgttaaaa gaagcaatag atgaattaaa aaacatccat tgcggggaag
    27661 tttttttatt aaaagaattg ttcaaaggct atgaatggaa caggttggag atgaatatca
    27721 gattgaaatt aggaaaactt tttttgtatg aagtggtaac taatcccaat ttaaatgtag
    27781 aggtcatcaa taaaaattct gcttatcagc aaatgtataa aaaaaaatag tgacttaaat
    27841 gtatataata gtataatctt cttattgatt caaaagttaa aacttttaat acaaaacaat
    27901 cattctattt taacatttat caatatgtct aaatttatat aattaacaca tacgaatagt
    27961 attgtttaaa tcacattatt taagatagat aaatcttaac cctactccag cataaatact
    28021 aaaatagggc ttagaattat ctatattttt atcccataaa atgcttagct cttcctcttg
    28081 attgaacata tatatctggg gaaatacaat agaagcgtaa atttataaaa tttccaaagt
    28141 cagaaaactt cagttcaaac ttaggtatac caaaactttc aggtgctatt tcgtcaacgc
    28201 caaaatagat gataattcca ttatctgtaa gataaaatgc ttggtcttca ggtataacta
    28261 actcaacatt agaataatac atttctctat tttcatttat ttgagattta atgtaatcag
    28321 taattatatt aatataatca actccatctc taaaaatgtc attaagtaaa agttcttttc
    28381 cagttgtcaa atctatattg taattattca atgtattgta tttaggccca tagttattag
    28441 caaaccccat aaggtttaat atcatactca gtacataatt tttattaaaa gtaactgcgt
    28501 aactggtaga tacccaatag ctttgttttg gaagtgaatt ttgagaagtt gcgttattat
    28561 attctcgttc ttcttcctgt aagccatttt taaaattatt tacagtcatt cttacagcat
    28621 tgtttatgtt atttaaaata actgggttta catacgtgga ttgatttcta atattgtata
    28681 taacaggata ctttacatca tatgaaaaat acttacttct ttcaaaatct tcattaaatt
    28741 gtacatataa tatattatat gtacaggtat ttgtagattg ataattttcc attaaaatcc
    28801 tccccctttt ataaaaaaat cagatacaat ataatatgct taaaacagta ataatgttaa
    28861 tggtaaatgt aaagaataga aagtatctaa aacagcaaaa aatgactaat tttgtggtat
    28921 aatacatagg attgagagtt attttatagg aggaaaaata tgttattatt agcagataaa
    28981 tggaaagatt atgaacttat agatatggga aatggagaaa aattagaacg ttggggaagt
    29041 tatgtactta gaagaccaga cccacaggtt gtatggccaa tggaatctga atgggcttta
    29101 tggaaaaacc ctcatggaca ttatcacaga agcaataaag gtggaggtca atgggaacac
    29161 aaaaaaagat atccagaaca atggactata aattataaaa acttaaagtt tcacattaaa
    29221 ccaactggct ttaaacatac tggattattc ccagagcaag cagctaactg ggattggatg
    29281 atagagaaaa taaagaaagc taacagacct ataaaagtgt tgaatctttt tgcatataca
    29341 ggtggagcta ctgtagcatg tgcatctgct ggtgcagaag tatgtcatgt tgatgctgca
    29401 aaaggtatgg ttaattgggc aaaagaaaat atagaattat caggtcttaa aaatcaaaca
    29461 gtaagattta tagtggatga tgttgtaaag tttgttgaaa gagaaataag aagaggtaaa
    29521 aaatatgatg caataataat ggaccctcct tcttatggaa gaggtcctaa aggagaagta
    29581 tggcaaatag aagaaaagtt atacagtttt gtagatttat gtatgggagt tttatcagat
    29641 aaccctttat ttttcttaat taattcttat acaacaggat tttctccaat agttcttgaa
    29701 aatgtattgt ctacaacaat aggtaaaaag acaaatacag gagatatata cggtggggaa
    29761 ttaggaatac cagcttccag agatggtaag atacttcctt gtggtatatt tggaagatgg
    29821 gagaaaaaat aatgataaaa gtcatatatg aagataacca tttattagtt gtagaaaaac
    29881 cagttaacat attgtctcaa ggagataata ctaatgataa agatatggta aatcttttaa
    29941 aagactatat taaaaaaaag tataacaaac ctggaaatgt atatattggt ttaatacata
    30001 gattggatag accagtaggt ggagttatgg tgtttgcaaa aacatcaaaa gcagcatcta
    30061 gattgtctga gcaggtaaga agtaagactt tcaaaaaaac ttatctagct gttgttcatg
    30121 gcaagataaa atctaactca gatatactaa aagatttttt atataaaaat aaaaaaacta
    30181 atatggtaac tgtagtgaaa aaaagccata aagatgccaa agaagctgaa cttaactata
    30241 atgtattaga ttttaaagaa aatctaagtt tagttcaagt taatttaaag actggtcgtt
    30301 ctcatcaaat aagagttcaa tttgcaagca gaaaacatcc attatacgga gaccaaagat
    30361 atggcaaaga aataaataag attggacaac aaatatctct ttggtccaat aagatagaaa
    30421 tttgccatcc tactacaaaa gaaaaaatgg aatttacatg tcatccacct gacaaatatc
    30481 catggaatat attttaaatc tcaaatgagg tgattatatg agtaaaaaat tttcaatagg
    30541 agaaatgtct aaacttcata aaataagcat acagacacta aggtactatg accaaataga
    30601 tttatttaaa cctgttcatg ttgaccaaga aagcaattac agatattact caattgacca
    30661 attttcacaa ttagatatta taaaatattt aaaatattta ggtatgcctt taaaagaaat
    30721 taagtcaaag cttgatgaaa ataatgaaga aattctaaaa ttactaacaa ataaaattaa
    30781 tatagtggat gaaaaaatta aagaattaga gcttataaag aaggtactaa gcacaaagaa
    30841 agaaactatg gaaaaaggta tgaaagtaga tgaagttggt aaaatagtga gaaaacatat
    30901 tccacgaaga agtatcttat ctatagagta tgaagaagga ttaagtagtg aagaaactat
    30961 ggaactttcc agaagacaaa tagcaaatat attagaagat aatatatctg tgttctatgg
    31021 tggagtaagt ggacttgtat ctatagataa atttttaaaa gaagataaag ttatttatga
    31081 caatagtttt gttactgttg aaagagactt atttgatata aaatcacaaa aacatataac
    31141 agaaatttca tctggtgatt ttatttgtat gacatataaa gggccttaca aagaaaatta
    31201 taccgctttt aagaaactgg ttgattatgt tgaatataaa agaataccta ttgaaaatta
    31261 tatatatgaa atacctataa tagaccctct gtctacaaac aacgaagagg aacttctaac
    31321 agaaattcaa atacaagtta aaaagagaga aattgactta agatgtctag tgtagtaatc
    31381 tagtttactt acacatctta atatagttgt ctattatcaa catatgattt ttctagatag
    31441 tgattcctaa cagtgagatt ttcaaaaaat aggaggaaat atggctaatg ctaattccct
    31501 cctattttta attaagccat atcattttaa ttaagccatg tcatcacgaa gtgcatcaat
    31561 aatattttct ttttttatct tattaatagt atacaacatt gtaataaata ctataaataa
    31621 aacactaaat atactaattc caatactaaa ccatggaatc acaaaatcaa tatcatctgt
    31681 aaccattact ttatgaatta gccatgaaga aattactgct ataggaagtc caaagaacaa
    31741 tgctctcatg ccataaaagg cacactcaaa attcatcatc ttttggaaat catgctcaga
    31801 cattcctaca gagcgaagca tggcaagttc tctacgacgt aacttaatat tagttgaaat
    31861 tgtattaaac acattggcaa tcgaaatcag agaaatcata attatgaaag tataagcaaa
    31921 aacattggca ataaaaatgt aattacggtt ctcatccatc attccataca tattgtacag
    31981 attgtactca ttcgtaactc cctcactctc aataatttgc tccatttctg caactgactg
    32041 agtaggattt ttcgattgaa agcttaaact ttcaattcct gtagaaacac caggaatctc
    32101 aaacttatct ttggctgaat aaggagccaa tactctgaag cttgctttct tcacatctga
    32161 acttctgatt cctggaggag aatcaggtgg cactactttg acaaatgaaa tattcacatt
    32221 ttgtctctca ggtatctcca acttgccatt agtttcagga gtgaggctaa cattcataga
    32281 agaactgttg aaaatatcaa taatattatt atcctttgtt accatagaaa cagcaatcag
    32341 ttttgcattt tgtccagtat attcttctgc tggtaatcca agacttttaa taatattcag
    32401 ataagttcta tcatcaagaa attgcatatc tactggtaat tcaagcattt cactcataga
    32461 atgtgaaccc tcatagttca aataatcgtc tgtaagctca cttactttgg caacacatga
    32521 ataggtcata agtttctgat acaaaccttc gtaaacacca tttgcagttt ttagcttatc
    32581 gtaaagcttc aacatttcgt tgtctttcat accttgtgta gaaaaaataa tatcctggtc
    32641 agtaacctct gacctctgat ttgacatctg ttttaggtct gctactaaag cacttgtaga
    32701 tataaataga actatgctta aaacaagcga caatacaata cttcgataac gtttcttgtt
    32761 tcttttaaag tttttcatag caagaactcc ctcaagacca taaactcgct gtgttagttt
    32821 tgatgtcttc acagatttag attcaatttt tacctcattt gtctgacgaa tacactccat
    32881 cactggtgtg ttggcagcct ttcttgctgg tatgtaagca gaaattaaga ttgtaaccat
    32941 acttaccact gctgcaacga tgattgcagg gatagacaat gttaatgtca acggaacgtt
    33001 tacatagaga atatttgcaa agtttttagc tatgacagaa atcacaagtc caatactgac
    33061 tataccgatt gcaacaccaa ttggtatacc aatcgcacca atgcaaattc cttcaaatag
    33121 caccgaattt cgtaactgtt ttgctgtagc tccaacagat gaaagtattc caaattgatg
    33181 tgttcgctcg ttcaatgaca tgttaaatga attatatatt agaaaaattg agcctagcat
    33241 gattatggca agcacaatgc caccaaccga gtacaaaaac atattgaaca aattgttatc
    33301 agaaacacct atgaagcgca acacattgtc attgaaaatg gaagcatggt ctttagtgct
    33361 acttatgtaa gagcgaatct gatgtggatt tttcaatgtg acaaaaaggc taagactgtc
    33421 agtcttgact tgtttatctt ctttggttat caaggtatat cctggtgcag aagattcttc
    33481 aaatacgggc tttctacaga tacccacaac tgtataagtt ttcttatcct gtggcacaaa
    33541 aacttcactc ccagcagtgt atgggtcact ctgaccaagc ttttcatttt cactcattcg
    33601 tttccctaca tccaaagaaa tagtatcacc tactgcaaat ttgacaccac catctgtcgc
    33661 aactttactt gaaacaagaa tctctctact attttcaggt aatcttccag aaattaaagt
    33721 tataggcaaa gtatcaaaag cttctttgct aaatccagca ataaaaagat atggtttatc
    33781 tggatttctg ttaccttcaa gtgttgcata gccaatattt tcaaatgttg cagtatttgc
    33841 aacttcatca ttgactcttt gttcctgtac aaaagaagaa tccacatcca aaaactggac
    33901 atgccaatca ccatatttta taatagctcc atttgtcata tagttcatca aagaaactcc
    33961 aaaggtagta actgctgtaa tcatagcagc tgacagaaca actcctataa ttgttacaag
    34021 agttcgagtg cgattctttt tcaatccttg cagagcaact ttgttaaaaa tgttcattaa
    34081 cgattcacct tcctctcatc acgcactact tttccatctg aaatacagat aattctatct
    34141 gcctgtaaag caatgttttc atcatgtgtg acaataatga gagtttggtg atatttacta
    34201 tggctctctt tcagtaactg gataatctct tgtccatttt tactgtctaa gctacctgta
    34261 ggctcatcag caagcataac tgctggtgca ttcatcaatg cacgaccaat ggcaacacgt
    34321 tgctgttggc caccagaaag ttgatttggt aaatgtgttt ttcgttcttt taaaccaagc
    34381 aattctaaca agtcatttaa ccgttcttga ttgaccttac gcttatccat aagtataggc
    34441 aatgtgatat tttctactac atttaatgtg ggtatcagat tgtgaaactg ataaatcaat
    34501 ccaacttgtc gcctacgaaa aatagcaagt ttttcattgc tttggccata tacatcttgc
    34561 ccctccagat atatctttcc acttgttggt acgtctactc ctgcaatgga atgtagtagt
    34621 gtagatttac cagaaccaga agaaccaatg attgcagtaa actctccttt ttcaatagta
    34681 agtgagacat ggtcaagtgc agtaacctta ttttcattct taccataaac cttacataaa
    34741 tcttcaatct ttaaaaacgc cattatataa gtctcctttc atattttata aatatatcat
    34801 aggactttca acttacattt aagtgacatt taaatgagag attcgtcact ttggaaaacg
    34861 aataacaaat attgcaccac cttgtgggtg attttgtgca gtaattgttc ctccttgtct
    34921 cataataatc gtcttacaaa gtgccaatcc tattccataa ccggctgtgc ttgaattttt
    34981 cccacgataa aatctgttaa atagacatgg taaatcttct tttttaaagc ctgctccgtt
    35041 gtcatgaata gtaagttgag taaacaaaaa gttgtcctca caaatgatat caatttttcc
    35101 attatcaccc acactctcca tgcagttttt aaatatattt tgaattgctt ctgaaagcca
    35161 atttaaatca cccaaaatcc ttactccttt tggtatatca atatgtaaag tgatattgtg
    35221 cagttccatg ggaatcaaaa gttgatgaag tgcagaacta atcaaattat tcacatcaat
    35281 ttgttctttt tgaaagacta caatacctgc atctaaacgt gataatttta aaagggaagt
    35341 aatcagccag tccattcgta taagtaattc ctctgtttct cgtatcaact cttttctctc
    35401 atcttcatca gaagtattct ctaacagtga caaaataaga gttactgatg taagaggagt
    35461 gcgaagctgg tgggctatgt cagctagtga gtcagcaagg tgttcttttt cttttttcaa
    35521 tgcgtaattt tgctctctaa tacgtaacgt cattttagtt atttcacttt gcagaataga
    35581 aagttcaccc tcatctgatt cagcaatata cagatggtta gcattatgaa gcacaagatt
    35641 aatttgttct gaaatctgtg caatactttt gtatcgattt ttagtaaaca caaaaaatgc
    35701 tattccaaag gtagtggcag aaacaataga aagtattcca gtcataatac tgattgcaaa
    35761 tcccagggct acagtgagaa tagtaattaa taagaataaa atagcaaatt ttcgaaactc
    35821 tctatttcga aacataactt ccccccaatc tataccctgt cccacgaaca gtcaaaatta
    35881 tttgtggact tgaagggtca tcttctatct tctctcgcaa gcgtttgatg tacacagtca
    35941 atgtgttgtc attaacaaac tcacctgccg catcccacaa ttcatcaagt aatcttaccc
    36001 tcgtaataat acttttagga ttgctaataa acaccaataa caaacggtat tctaaagctg
    36061 aaagaaaaac ctcattgcca ttctttttca caacaccggt tgccgtatct acataaagcc
    36121 cacaaatttc aaaagttgac ccaaaacgcc cactttttcg tagtgctgtt ctaattcgcg
    36181 caatcagttc acgtggacga aaaggtttag taatataatc atctgcaccc atattcagtc
    36241 cagtaacaac acttgattca tcaccggaag ctgtcaaaaa gataacagga atgtcttgct
    36301 tttctttgat ttctgtgcaa atcgtaaacc catttccatc aggtaaagaa atatcaataa
    36361 gtgccaaatc aaatttattc ttagcaatct ctacaagtgc ttcacttctt gtggatacat
    36421 gggttactat aaatccttct gaattaagca aaagaataag atttttggca attgctttat
    36481 catcctcaac taaaaatatc cgtttcattt actttcatcc tcctttattt tattagtttt
    36541 atatattcca gcagagcaat agtacactac tattttactt atcattacac aattttcagt
    36601 agtttctttt gttcttatat aatccatgca taatacctcc attgatttat tatatttctt
    36661 ataacataaa tacacaagat aagacaaaaa acataatgta agtgcaagtt ttttaactca
    36721 ctaacttata aacatacact cctactaaca ctactttctt gatgacacat cttacaagct
    36781 tatattcata catcaatgaa aaattaaatt tatccattta ttaaaataaa aggcaatagc
    36841 ttctatagct aatgcccttt atcttaaaat gatttaaata ctatacaatt tttataaatg
    36901 gctataaata aactcgtata gaatataaaa tttatctaca gtcttttgtt atacaaaatt
    36961 tataacctct tatagaacaa atttaccact ttgcaaactt atcactttta ctcagctata
    37021 ttataaactt ttaatttatc tttgtttaaa tgtttcttct taacttctac taaagctttt
    37081 agcgtatcca aagatgtcat tatttcagta gaaaattcaa tggctgttct tctcatctta
    37141 aatccatctc ttgtagagtc atttccttta gttggtgtat ttattactat atcaacttgt
    37201 ttatttctta tagcatctaa tatgtttggt ctaggctctt caattctatt tactatatca
    37261 gcctctattc cattttctct taaactcttc gctgttcctt ctgtagcaac aaaagtatat
    37321 cctaattctt tcatatcttt tgctatctct ataaactcat ctttatcatg attatttaca
    37381 gtagcaagta ctactcctct ttcatcagac atatgtctac ctgctgccaa gaacccttta
    37441 tataaggctt cttctaaatt ttctcctaca cctagaactt ctcctgtaga cttcatttca
    37501 ggtcctagac ttacttcaac tttagataat ttagacattg agaatactgg tacttttact
    37561 gatactaatt ttggctcttt gtaaactcct gtactatatc ctaaatcctt taatttagct
    37621 cctaacatac acttagtagc caagtcaact attggtactc cacttacttt acttatatat
    37681 ggaactgttc tactagctct agggttaacc tcaattatat ataattcatt ttgaaattct
    37741 ataaattgta tattaaccat accaagcaca ttaaggtcta aagctatttt tttagtgtat
    37801 tctactattt tttcttttat ttcatcagat atattttgac ttggatacat tgttgtacta
    37861 tctccagagt gaactccagc tctttctaaa tgctccatta taccaggtat caatatatct
    37921 tctttatcac atatagcatc tacttctatt tctctaccag ttagatattt atctataagt
    37981 actggatttt tatggtctct atcaaatgca tcatccaaat actgaattag tttttcttcg
    38041 ttataagtta tttccatacc ttgaccacca agtacataag atggtctaac taatactgga
    38101 taacctaact ccttagcaat ttcaacacct tcattagttg tccatacacc ttttcctttt
    38161 ggtctattta tatctagtct ctctagtaaa tcatcaaatt tttctctatc ttctgctgca
    38221 tctatatctc taaagtctgt tcccagtata ggtatatttt tttcatgtaa aaacttagcc
    38281 aattttatag ctgtttgtcc accaaactgt agtattacac cttctggttt ttctttttct
    38341 attatattta aaacttcttc ttctgttaat ggttcaaaat acaacttatc agatgtatca
    38401 aaatccgtac ttacagtttc agggttattg tttacaatta tagtttctat tcccattttt
    38461 cttaaagatt ttacacagtg aactgaacaa tagtcaaatt ctattccttg acctattctt
    38521 attggaccag aacccaatac tacaactttt ctcttatcac taactactac ctcatcatat
    38581 tgttcataag ttgaatagta ataaggtgat agagcttcaa attctcctcc acatgtatca
    38641 accattttat aaactggttg tatattatat aaacttctca attcatataa tttttctggg
    38701 cttatcttca ttaagtcaga tataccttta tcagaaaatc cctttttctt taattcatgt
    38761 aaatattctt tatctaagtc ttctattttt agaactttta acttttcttc ttgttctact
    38821 atccatttaa atttatttat aaaccactta tcaacaccag ttatttctga tatcatttct
    38881 actttatagc ctcttcttat catttcagct aagtcaaata gtctctcatc atcaggtact
    38941 actactctag cctttaattc ttctaatgtt ctaccttctg atggagcatg aaccaatgaa
    39001 tattttcctg tttcaagtga tcttatacct ttaagaatag cagcctcaaa gttacttcct
    39061 atactcatga tttcaccagt tgccatcatc ttagttccta attttctatt ggcatgtcta
    39121 aatttatcaa atggccactt tggtattttt actaccacat aatctaatgt tggctcaaaa
    39181 caagcataag tttttttagt tactgcattt tctatctcat ccaaagtata acctaaagct
    39241 atcttagctg ctacttttgc tattgggtat cctgtagctt ttgatgctag ggctgatgac
    39301 ctactaactc ttggatttat ttctattatc gcatattcta aactatgtgg gtttaaagct
    39361 atttgaacgt tacatccacc ttgaacctct attgcattta ttatgtctat tgatgccttt
    39421 ctaagtagtt gatactcttc atcacttaga gtttgacttg gagcaacaac tatactgtct
    39481 cccgtatgaa ctccaactgg gtctacattt tccatattac atacagttat acagtttcca
    39541 tttccatccc tcattacttc gtattctatt tctttccaac cttttatact cttttcaaga
    39601 agaacttgtc caacaggact tagctgtagt ccatgtgaaa gtatttctct taattcttcc
    39661 tcagtatcag ctatacctcc accagttcca cctagcgtat atgctggtct tacaactact
    39721 ggatatccta tcttattggc aaactcaaga cctgcttcta aatttgtaac tatatcacta
    39781 actattactg gttgatttat ttctttcatt acttctctaa ataaatctct atcttcgccc
    39841 ttttttatag attctataga tgttcctata acttttacat tatatttatc taatatacca
    39901 gcatcatgta attctacagc taagtttaat cctgtctgac cacccatacc tgcaagtaaa
    39961 ctatctggtt tttctttttc tattattttt tctataaatt ctattgttaa tggttctata
    40021 tatattttat ctgcaatttc tttatcagtc attatagtag ctgggttact atttactaat
    40081 acaacttcaa ttccctcttc ttttaatgct tggcaggcct gtgttcctga atagtcaaac
    40141 tctgcagctt gacctattat tattggccct gaccctaata ctaaagtttt ctttatacta
    40201 tctaatttag gcataactct ttctccttat ctaaacaatt ttcacagttc ttatcttcat
    40261 catctataca atcaacttta attactttat tttatcttaa attcaaattt actcatcaat
    40321 acatctattt aatacatcta tgttaattaa accaaacttt ttgatttaag ttattcttgt
    40381 tttttagtta gctattctaa tgttatttat tttactaatt ctaaaaattt atcaaaaata
    40441 tagtcattat cttttggtcc tgggcaagct tcaggatggt attgaacact gtatacatct
    40501 agtttctcat gtctcattcc ttcaacagta ttatcattta aatttatatg tgtcactttc
    40561 atttgttcag gaacttctga aacataataa ccatgatttt gagaagttat aaatactttt
    40621 ccttcttcta aatctttaac tggatgattt ccacctctat gcccaaattt aagtttagca
    40681 gtttttcctc caagagctaa agctaaaagt tgatgtccta agcatattcc aactataggt
    40741 tttttaccaa taagagcttt tatattttct ataacatctt ctaggtcttc tgggtctcca
    40801 ggtccatttg acaagaatat taagtcaggg tttatactca atacatcttc tggagatgtt
    40861 gtagctggaa atattgttat atcacatcca cgagctataa atgatcttaa tatatttctt
    40921 tttactccaa aatccataac agccactttt ggaccatttc cctttatatg ctctacttct
    40981 tttcttgtaa cagttctaac tgcttctgta tttgaaaact tatccaattt atgtttaaca
    41041 tcttctaatt tactgtcttc taatgtaatt atacctttca tggttccatt atttctaagt
    41101 atcttagtca aagctcttgt atctattcct tcaagtccga taactttatt ttgctttaaa
    41161 tattggtcta tatcaatttc acatctaaaa ttacttggag aatcactttt ctcccttact
    41221 atgaatccct ttacttgaac tttttcagac tctgctgatg ataaatttat accataattt
    41281 cctacaagag gataagtcat agtaactatt tgaccatagt aagaagggtc tgttaaaact
    41341 tcaccatatc ctatcattga agtattaaat actacttctc ctacactttc ttctaaatat
    41401 ccaaatgcct ttcctataaa aactgttcca tcttctaata ttaaccttgc tttcatacta
    41461 cagtacctcc tataattctc tttagacaaa ctaaagaaca ttaactaaat ataagctttt
    41521 aaatatctat atctattttt cacttttaca ttctagtttt atacttattt acatttattt
    41581 tttatctttt tctattatac tacaaatttc ttaaaaaaaa gaagttatct caaaagtaaa
    41641 aactcctaga aaaaataacc tctttttttt atttaataat taaattctta aactatacaa
    41701 tcaacttaat ttctattcag ctatattgta aacttttaac tcatctttat ttaaatgttt
    41761 cttcttaact tctactaaag cttttagtgt atccaaagat gtcattattt cagtagaaaa
    41821 ttcaatagct gttcttctta tcttaaatcc atctcttgta gagtcatttc ctttagttgg
    41881 tgtatttatt actatatcaa cttgtttatt tcttatagca tctaatatgt ttggtctaga
    41941 ttcttctact ctatttacta tatcagcctc tattccattt tctcttaagc tattagctgt
    42001 tccttctgta gcaacaaaag tatatcctaa ttctttcata tcttttgcta tctctataaa
    42061 ctcatcttta tcatgattat ttacagtagc aagtactact cctctttcat cagacatatg
    42121 tctacctgct gccaagaacc ctttatataa ggcttcttct aaattttctc ctacacctag
    42181 gacttctcct gtagacttca tttcaggtcc tagacttact tcaactttag ataatttaga
    42241 cattgagaat actggtactt ttactgatac taattttggc tctttgtaaa ctcctgtacc
    42301 atatcctaaa tcctttaatt tagctcctaa catacactta gtagccaagt caactattgg
    42361 tactccactt actttactta tatacggaac tgttctacta gctctagggt taacctcaat
    42421 tatatataat tcattttgaa attctataaa ctgtatatta accataccaa gcacattaag
    42481 gtctaaagct atttttttag tgtattctac tatcttttct tttatttcat cagatatatt
    42541 ttgacttgga tacattgttg tactatctcc agagtgaact ccagctcttt ctaaatgttc
    42601 cattatacca ggtatcaata tatcttcctt atcacatata gcatctactt ctatttctct
    42661 accagttaga tatttatcta taagtactgg atttttatgg tctctatcaa atgcatcttg
    42721 taggtattga gataattttt cttcgttata agttatttcc ataccttgac caccaagtac
    42781 ataagatggt ctaactaata ctggataacc taactcatta gcaacttcaa taccttcgtt
    42841 aagtgaccat acaccctttc cttttggtct atttatatct agtctctcta gtaaatcatc
    42901 aaatttttct ctatcttctg ctgcatctat atcatcaaaa tcagttccca gtataggtat
    42961 atttttttca tgtaaaaatt tagccaattt tatagctgtt tgtccaccaa actgtagtat
    43021 tacaccttct ggtttttctt tttctattat acttaaaact tcttcttctg ttaatggttc
    43081 aaaatacagc ttatctgatg tatcaaagtc tgtacttaca gtttcagggt tattgtttat
    43141 gattatagtt tctatgtcca tttttcttaa agattttaca cagtgaactg aacaatagtc
    43201 aaattctata ccttgaccta ttcttattgg accagaacct aatactatca cctttttctt
    43261 atcactaaca actacctcat catattgctc ataagttgaa taatagtaag gagatatagc
    43321 atctatctca cctgcacaag tatcaaccat tttatatgct ggtttgatat tgtatagact
    43381 tctcaattca tataattttt ctggacttat cttcattagg tcagatatac ctttatcaga
    43441 gaatcccttt ttctttaatt caagaagata gtctttagtt aaatcttcaa ttttcataac
    43501 ttttaatttt tcttcttgct ctactatcca tttaaattta tttataaacc atttatctat
    43561 acctgtaatt tgttctatca tttctacttt atagcctctt cttatcattt cagctaaatc
    43621 aaatagtctc tcatcatcag gtactacaac tcttttcttt aattcctcaa tgcttctctc
    43681 ctcagatggt gtgtgaacta atgaatattt tcctgtttca agtgatctta tccctttaag
    43741 aatagcagcc tcaaaattac ttcctatact cataatttca ccagttgcca tcatcttagt
    43801 tcctaatttt ctatttgctt ttttaaattt atcaaaaggc cattttggta tttttgccac
    43861 cacataatct atagttggtt caaaacaagc ataagttttt ttagttactg cattttctat
    43921 ctcatctaaa gtataaccta aagctatttt tgatgaaact ttagctattg gatatcctgt
    43981 agcttttgat gctaaagctg atgacctact aactcttgga tttatctcta taactgcata
    44041 ttcaaaacta tgtggattaa gtgcaaattg aacgttacaa ccaccttgaa cttctactgc
    44101 atttaatata tctaaagatg cttttttaag cattgcacat tctttgttac ttaaagtttg
    44161 agttggagca acaactatac tgtctcctgt atgaactccg actgggtcta tattttccat
    44221 gttacataca actatacaat ttccctttga atctcttatt acctcatatt ctatttcttt
    44281 ccaacctttt atactctttt caattaatac ttgtccaaca ggacttaatt gtagaccatg
    44341 tgaaagtatt tctcttaatt cttcctcatt atcagctata cccccaccag ttccacctag
    44401 tgtgtatgct ggtcttacaa ctactggata accaattgta agtgcataat caagacctgc
    44461 ttgtaaatct gtaactatat cactaactat tacaggctga tttatttctt tcattacttc
    44521 tctaaatata tctctatctt cacctttttt gatagattct actgaagtac ctattatttt
    44581 tactccatat ttatctagta tgcctttttc ataaagttca actgctaggt ttagccctgt
    44641 ttgtccaccc atacctgcaa gtaaactgtc tggtctttct ttttctatta ttttttctat
    44701 aaattctatt gttaatggtt ctatatatat tttatctgcc acttctttat cagtcattat
    44761 agtggctgga ttactattta ttaatacaac ttcaatccct tcctctttca aagactggca
    44821 agcttgtgtt cctgaatagt caaactctgc agcttgacct attattattg gtcctgaccc
    44881 taatactaaa gttttcttta tactatctaa tttaggcata aatttttctc cttatctaaa
    44941 tggtttttac tatctgtaaa taatactaaa tctatattaa tgctaagaat ttattaaata
    45001 tacaatctaa atcttctttt gttgtgtatt ctggaatgta ctgaacacta tatattggta
    45061 attccttatg tctcattcct tctatagtat tgtcatttaa atttatgtga gttacttctg
    45121 tattttctgg catttctgaa acattatatc catgattttg agaagttata aatacctttc
    45181 cagtttctaa acatttaact gggtgattac ctcctctatg tccaaataca agctttgaag
    45241 tctttcctcc taatgccaat gctaaaattt gatgtcctaa acctattcct gtcaatggct
    45301 ttttgcctat taattttttt atattttcaa ctatatcatt tatatcctct ggatttcctg
    45361 ggccattaga taataagatt aagtcaggat ttatatttaa aatttcttct actgtagtca
    45421 ttgctggtac tatagttata tcacagttac aagccttaag agactctagt acactatttt
    45481 taacaccaaa gtctataact acaactttag ctccagtacc ttttatattt tcaatttctt
    45541 ttcttgtaac attttttact gaatctttgt tacaatgctc ttctaattta gactgaacat
    45601 cttgtaatga tacattttct aaagttataa ttcctctctt agttccattg tttctaataa
    45661 ttttagttaa tgctcttgta tctattcctt caagtcctat taccttattt tgctttagat
    45721 atgtgtctat atccatttca catctaaaat tatttgggtt atcacttttt tctcttacaa
    45781 taagtccttt tacttgcacc ttatttgatt caacatcttc taaatttatt ccatagtttc
    45841 ctataagtgg ataagtcata gtaactattt gtccataata agttgcatct gtcaatagtt
    45901 ctccataccc agccattgaa gtattaaata caacttctcc aatagcttcc tttagatatc
    45961 caaatgcttt tccttcaaat actgttccat cttctaaaat taatttcccc ttcatgtaag
    46021 ctacctccca aatattttat ctaagcattt tctatttctc ttacaatatt aatagctgat
    46081 tctcttggat tttctgcttt tgttataggt cttccaacaa ctaaataatg agcacctttt
    46141 tttattgcat ctgatggagt tacaactctt ttttggtctc caacctctgc tgatttaggt
    46201 cttattcctg ggcaaactgt tataaaatca gttccacatg cttctactat tttatctact
    46261 tcttgcgctg aacaaacaac cccatctatt cctgattctt ttgccagttt agctctcttt
    46321 atagctaact cttctgttgt catattacat cctatgtcct gtaaatcttg gttgcttaat
    46381 gaagttaaaa ctgttactcc aactactaat ggcttttcta tatttaatct ttctgcttct
    46441 tccctagcaa ttagtgcaca ttgtctcatt ccttccatat ctgatacatg tattgtcatt
    46501 aaccaaacat tatctctcac tgcagctttc actgcacttt tcatagtatt tggtatatca
    46561 tggaatttaa ggtctaaaaa tattttttta cctttttctt taagataatc tattgtttta
    46621 ccttttgtgg caacgtattg ctctagccct accttaaata tgtctacact atcttctaat
    46681 ctgtctatta gttctttagc tttatcaaat tcgtcagtat caattgcaac tatcaatttt
    46741 tctttaccat ttatcatttt tacatctcct ttttaataaa gcctaaataa tcataaaaaa
    46801 atcctcacac caaaagaggc ataaggattt tatctataca taactctatt cattagctat
    46861 gagatatctt tcccagtctc tctggactag tttaaaagac tttattatat ttaaattttt
    46921 attttatact catcaaaaac ctctagaaga tttactagag gttatatact aacattatct
    46981 ataacccttt agtctctctg gactacttta aggaatttag ctgtaatatt tttctttatt
    47041 tatataactt tatgtatcta taggataatt taaagtttta tcttttttat aattaaacca
    47101 ctaattgctt tttatgtcaa taagtctttg aattattctt catctttatc aaacaatttt
    47161 ttctcaaaat atgattctgc attatatatt gcagcaactg gattatgccc agttactcta
    47221 tctttgacaa ctaaagttgt tataggagct tccatatatt tcatggctaa agtatcgtga
    47281 ccaacacata atccaagaat tacattaagg tcaaccttag cttcatttaa aaataaagct
    47341 tgtcctattg gattgcacat tacctcatct gaacactttg atattgtttg agatgcttct
    47401 agtccaattg tactcttagg cattgcccca tttttacata taacagaaaa tacttcaaac
    47461 ccatgatgtt caaatatttt ttgcactgtt tgcatttctc tttttagtcc aatacaaaat
    47521 gctatgccaa tctttttata accacacttt ttagcaaact ctataatctc ttttagtcta
    47581 gtatattgac aatacccctc tgcttcaact aatccagcat tataagctat ttttctattc
    47641 tcttcttctt cataaagttg ctttgctttt tgttgtagtt caatttctct agttggacat
    47701 atcttcatta atttatccat atttccatta gaacagcctc taagattaca ttttccacac
    47761 ttatacatat ccaatatccc cttccaaaat tttattcatc ttgcgattct tctttttcta
    47821 gagttacatt ttcttcttgg gtttcttctt ctggtttcac atgctcgact acagttataa
    47881 aatatgattt tcctatttta ttccttatag gaagtttttc atatgaaaca tctattttat
    47941 tttttataca agctatatca aaatgtctta aggctacacc taagctaacc ttattcatat
    48001 cttcacagtg tttcttaggt ctcttttcaa ataaatgaag acctcgttca tccttatgga
    48061 aataccacgg ttgagaattt tttacagatg gagctcttct tgtcaattct aagatatctt
    48121 tccactttct attcctattt atcttattta tcatatcttt tactggcttt ctatcaggtc
    48181 tagcatatct cgtcctaaac aactcttcat tttttgctgg atatccaaat gctattatta
    48241 tataaggatt ttctattttg ttttcatcat cttcttctgt ttttattcca gctagataat
    48301 ctaaatcatc ttcctcatct tcttcaaaat tcttattccc aatatctaca aattccaaga
    48361 tatcttctct ctttaagttt ccttccaacc agcaagtagc tattcccaat gttgtaaggc
    48421 gcaaaactac accttctgta gcaaatccta tattctgtaa atagtcctca cctttgtctg
    48481 atgtaactac tatataatga ggtgccttta ctttacattc cttacccatc aaaaaatgta
    48541 ttatatgacc tctatcaaca acatgtgctt taatatttaa atcttcattt aagtattcta
    48601 aatttttaca acttcttttt atttcttcca tcaatggctt ttttatcttc ttactggaaa
    48661 aatttcttac agattttcta taaaaaatgg cttcataaag ctccatttat gccctcctaa
    48721 ctgaaatctt ctgacgtata aactctataa atttatataa atactttata ttttttatag
    48781 ttttatttcc tttttcctcc tgccctattt tgaaattcat aagaatatct actctagttt
    48841 aatatagcat tcaaaaggct taaattctac cttatcaatg tctatacatt agcataattt
    48901 ttttatttga ttttctatgc taatttaaat aacaacacta gctattacct ttttaattaa
    48961 gctttgccag aaaaattttt tttcatttat aaatatctat ctcattatta tattataagt
    49021 ttttttcggt attcttgtat agcttttcac taaaaaagta tttttatttc tactttaata
    49081 ttattatcaa caaaaaacat actgcttaaa taagtttgtt atcctcataa tttgttttaa
    49141 ttaaaatatt ttttttattt ctcaatctat aaagtataaa tattttgaat atgacccaaa
    49201 atctaagtaa agttataata ttttataagt tgttcaatat taaaaacata agtaatgtta
    49261 taatacttta taagttattc aatattaaaa atataggagg ataaatatga atttaaatag
    49321 aaatttagaa aaatacgctg aattagcaat aaaagttgga gttaacatac agccaggtca
    49381 aattctactt ataaagtcac caatagaatg tgctgatttt gcaagaaatg cacttaaaga
    49441 agcatataaa tgtggtgcta aaaatgttta tatagagtgg tctgatgaag aaagtactct
    49501 tattaaatac ttatatgctc cagatgaagc atttcatgag ttccccaaat ggacagcaga
    49561 acaatatgta gatatagcaa aagaaggtgg ggcattttta tcagtctatg cccaaaatcc
    49621 agatttatta aaagatgttg accctgaaag agttgcaaat tttcagaagg catctggaaa
    49681 agctttaaaa gaatggcgtt cttacactct aactgataaa tgtaaatgga atatagtctc
    49741 tgtgccaaca aaagattggg caaaaaaagt gtttccaaat ttatcagaag ataaagcaat
    49801 agaaaaactg tgggatgcta tatttaaatg ttcaagagta gatggtcagg accctataaa
    49861 agcatgggaa gaacataatg aaaatcttaa atctaaaatg gactttctaa ataaaaataa
    49921 ctttaaaact ttaaaatata aatcctcaaa aactgatttg actctagact tgccaaaggg
    49981 tcatgtttgg ctaagtggag catccaaaga ccctaatgga ataagtttta atccgaatat
    50041 tccaactgaa gagatttttg gaatgccaca taagtttaaa gtaaatggaa ctgtttatag
    50101 tacaaaacct cttgtttatg gtggaaacat aattgacaat ttcttcttaa catttaaaga
    50161 tggtaaaata atagatttct ctgctgaaaa gggactagat acactagaaa aacttattga
    50221 aacggatgaa ggctctcatt atttaggaga agtcgctctt gttccataca actcaccaat
    50281 atccaatacg gatataattt tttataatac tctatatgat gaaaatgctt cttgccactt
    50341 tgcaattggt tctgcttata agacttgtct ggaaggtgga aataatttaa aagatgaaga
    50401 attggatgaa catggtgtaa atgacagttt aacacatgta gattttatga ttggttctgc
    50461 tgatatggat attattggtg agacttatga tggaaagcaa atacagattt ttaaaaatgg
    50521 taattgggct ttttaacata tgattttaac taaaaagggc tgatggaagt tataccagac
    50581 ttcccagccc tttttaatgt tgatgataaa tattttttat attaattaat ctcattatta
    50641 tagattaatt cctattgttt taaacataag tatctaaaac ttatatttat attatatata
    50701 aaatatccta tttagtaaaa cctttatttt aatttatatg ctaaaaattt aactttgcta
    50761 tttcaacagc agactttgca tcctttgaat aataatcagc acccatttct tcagcgtatt
    50821 cttctgtcaa aactgcacct cctacaaata cctttgcatt tatctcatta tctctcagtg
    50881 cctgaattgt atctttcata ctttgaacag tcgtagtcat aagtgcactt aatcctacta
    50941 attttatatt tctcttttta gcaacttcca ctacttcttc aatagggaca tctttaccta
    51001 aatctataac ttcatatcca taattttcta gcattatttt tactatattt ttcccaatat
    51061 catggatatc accttttact gtagcaacta ttatttttcc tttggataca ttattgctac
    51121 ttttacttaa taaagtttcc tttattgtat ttaatgaaac ttttaccgtt tctgctgatt
    51181 gaatcaattg tggtaaaaat atatctccac tatcatatct ttttcctact ttatcaagag
    51241 caggaattaa aatctcatct aatatataat tttcatcctt ctcttttaaa agattcagtg
    51301 ttacattttt tgcttcatct tttaatcctc tttcaactaa aatatccaag ggcaaatctt
    51361 tatcaatctt tgtagatgaa tccgattttt ttgaattatt tatattgcta tattgattta
    51421 tatagtttat acatccttta tctgtgttat ttaatatttt aaaagaattg atagcttcca
    51481 tcatactata ttcatttgga tttataattg ctaaatcaag ccctgcccct aaagctaatg
    51541 ttagaaaaga agcattaagt gctttcctat ttggaagacc aaatgaaata tttgaaacac
    51601 ctaatatagt ttttactcct agtgtcttta ccattgttat agcttttata gtttctattg
    51661 cttcttcttg ttgtgctgaa acagttaatg ataaacagtc tataaatata tcttttggtt
    51721 ttatcccata agaaactgct ctattaacta tcctttttgc tatttcaaat ctttcttcag
    51781 cctttttagg tatccccttt tcatcaagtg taagtccaac cacacatgaa ccatactttt
    51841 taactattgg taatatactt tctaaagact cctctttgcc atttacagag tttactattg
    51901 ttctaccatt ataatatcta agtccttgct ctaaggcctc tacattggaa gaatcaactt
    51961 gtaaaggtgt atctactact gcttgaatct ctcttattaa ttttggcatt agtttttttt
    52021 catcaatctc tggaagacca acatttactc caagtatttg tgctccagca tttacttgct
    52081 ctaaacctaa atttatagca taatcaacat tttcattttt taatgcttct tgtagagatt
    52141 ttctacctgt gggattcaat ctttctccaa caactgtagg cgattgtact tccacaaatt
    52201 ttgatggtga acagactaca catttatcaa ttttctcgat acatcccttt gtaacagaat
    52261 ttatattctc agatatttct tttataaaac ttggattcgt accacaacat ccacctatta
    52321 tagtagcacc aacttcaacg aactttttaa ccccttcaaa aaattctttt gcatcgacat
    52381 catatatagc ctctccatca actatatttg gaagacctgc atttgcttga accataacag
    52441 ggactgtagc tcttgatgct attttttcaa ctattggtaa aagttgttta ggtcctaatg
    52501 aacaattaac tcctattgca tctacaccta gaccttctat agtagccacc atacattctg
    52561 gcatacatcc agtaaaactt cttccacctt catcaaatgt catagtacag aaaataggta
    52621 aatcagagtt ttcttttgct gctaatacag ctactttagc ttcatataaa tccatcatag
    52681 tctctattac tattacatca actccagatt taacgccttg tagtacttgt cttttaaata
    52741 tttcatatgc tttatcaaaa cttaaagtac ccataggctc tagcatttct cctataggac
    52801 caatatctaa agctacatat cttggtttag tattatctac attttctata gcttttctag
    52861 ccaccaaaac agcattgtca atcacttctt ctacagtata cttactatct aattttagtt
    52921 cattacatcc aaaagtattt gtaagaatta cattcgaacc tgcctctaag tatgctgtat
    52981 gtatttctat aagtttatct ggattttgaa gaccaaatac ttctggattt tcacctaact
    53041 ttaacccttt ttgttgaagc attgttccca ttgcaccatc aaatattaat atattatttt
    53101 tcagatattg tcttatttcc acaagagttc gctccctttc tatatgtaca gtttctatag
    53161 tttttacaat tttcacacga cttttctttc ttagatttat ttattcttgt attagaaata
    53221 cctataatag ccactactga ctttctcggt atcataattc cattagaagt aattgttaga
    53281 cctatttgtt tttgagcatt caaaacgttt aatatatcta tatttttttc tattggtaaa
    53341 tctccatatc ctgggctata tcgcatagtt ataaatttat cttgctttaa aaaaaccttt
    53401 tctaaattat tttgaacaat gtcacacact tcttctattg ctgttgttcc acatgaatca
    53461 attattagtc cttttgttaa atttgaataa gagtgtcgtc ttatttcttt ttctatatct
    53521 atccctatag tagctgccat aattacacac tcattacatt cttttaaaag ttcatataca
    53581 tatttactct ctaatgttaa atttgttccc tctaattcta caacattatc tttatttatt
    53641 ctaataggat aaatcctaga taaatatcta ggatttattt tatttttagt tatagctaca
    53701 cattcatcaa tagtatattt aaggtcttta tctatctctt gacctctgta ttgtaaatat
    53761 cttaaaactt cactttgatt tatgcttata tcttctcttc caattaaatt attctccata
    53821 tatatttaaa gaaaatcctt tatctaaaac atcttttgca tattcctttg ccccaaataa
    53881 agataagtct ctgtagtttc cacattctat agctgtagct gctggcattt tatctgattc
    53941 aagaactttt tttaatgctt cttctaaacc aatcttaact gttttggcat ctacatctcc
    54001 ccatagtatt aaataaaatc ctgttctaca tcccattgga gataagtcta ttacaccatc
    54061 taatgtatct cttaaataag tagctaaaag atgctctaaa ccgtgcatag cagcagttcc
    54121 aaaactttct acatttggct gtaaaaatct caagtcaaat ttagttactt tatctccctt
    54181 tactccatca agtacactac actttcttac aaaaggagct tttacttttg tatggtctag
    54241 cttaaaactt tctacttttt ccattattta atcctccatt atactttaaa atttgttaca
    54301 ttaattctaa aaaaaactct tcctatctat tattgataag aagagtatat tatttcaagt
    54361 catctcatcg ttgttagcag gaccacctga taaaaggttg gtataggatc actgagctaa
    54421 ctctctcccc taactcttga taagtatctt attaaactga cttaaattat atattacttt
    54481 tttatttttg taaacacttt attgttataa tatttatatc acataatcgt acttttattt
    54541 tataactaag ctgattgtat tattggcagt tgtacttcaa acacagtttg atatttatcg
    54601 cttgaaacag taatttctcc atcatgtaaa tttacaatat tttttgtaat cgcaagaccc
    54661 aatccagaac ctccaacatt tctatttctc gatttttcaa ctctataaaa tctatcaaat
    54721 atatttggta aatctaacac tggaattggt tccccataat ttatcacttt tacaaccgcc
    54781 atatcttcct ttttctctgt gactatatct acataatgcc catcttttcc atatttcaca
    54841 gcattagtta taagattttc aaatgctctt accaatttat ttggatcagc ttttacaatt
    54901 aatttttctt cattaaaatt aattctacta accataaatt cttgtttaaa ataaatttca
    54961 aattgagata ctacttgact taataactct actaaattta tttctatttt gtttaatttt
    55021 attgtattat tttgcatttt agtcaattca aacaaatcat ttataagaac atttaaattt
    55081 ttcgattttt catatgcaat atcaacatag tacatcatct gaacttcatc tttatactta
    55141 ccttcttcta tcactcctaa ataacccatt atggatgtca aaggtgttct taagtcatgt
    55201 gacacatttg ttattaaatc tgtttttgtc tgctgagctt gtctctcttc aatagttata
    55261 tttctaagtt gatggactat attattgatt ttatgtgcta gttcatcaat atcatccttt
    55321 tctttatcct ttaatatatc taaatcatac tcaatatcta tacttttttc taagttacct
    55381 tcatccatta aatttacagc acttttaatg atttctaatt tttgattttt tctattagtc
    55441 tttcttataa aatctacaat ttgcttattt ctcttgtacg atataatgga ataaattgct
    55501 aatgatgtga ttgtaaataa aaatggataa aataaatcat accctataag acttctgaaa
    55561 tctcttgctt gatatacaag ttgattaact acagacatat cactattgac atataatgat
    55621 tttattatat acatacttgt taaaataagc attgaagtta aagcacagct tattataaga
    55681 tctaatatgg gtcgaaacat atgatttaat aaacttttat ttttcaattt tatatcctac
    55741 tccccataca gtatgaatta ttttttctcc atctgtatat ttttcaattt tatcccttat
    55801 tctgctcata tgaaccatta cagtattatt agattggtaa tatttctctt tccatacctt
    55861 ttcaaatatt tcttctgtac tatatactct gcctttatta tttgctagta aatataatat
    55921 atcaaattct cttgatgtca aaattatttc atttccagcc actgttacac tatgtttttc
    55981 cttatctata attactactc caacctttat gacatcttta tcttcattat tagcaccact
    56041 aaaataatat gctcttctaa tcagcgactt cactctcaca gtaagttcaa gtgggttaaa
    56101 tggttttgtc aaatagtcat ctgcaccagt cattatccct tgtattttat ccatgtcttc
    56161 tgattttgca cttaacataa gtattggtat attcaaactt tctctaactt ttctacaaac
    56221 ttctatccca tccattttag gcatcattat atccaatatc attaaatgaa tattttcctt
    56281 ttttaaaata tctagagcct cttttccatc ttcagcttta attacattat atccactatt
    56341 taaaagatat atctctataa ggtctcttat ttccttttca tcatccacaa caagaactgt
    56401 attattcatt tttacacatc ctaacttaat ctttgtatat agctttattc atataaaatt
    56461 atactatatt ttagttagtg taatctataa atttattaat ttctaaattt ccaatataat
    56521 acaaattatt tacacattta tattatttat tttgtaaata ttcttctaaa gttaatcctt
    56581 ttgaataaat ttcctcagca gcatccttac caacatatct aatatgccat ggttcatatg
    56641 ctgtacctgt tatatcttct ttaccttcag gatatcttat tataaatcca aacttgtgag
    56701 cattttctgc caaccattta gcttcttttg tacttccaac aaaccatctt tctggatttg
    56761 ttaaatcaat acaaagtcca gtttggtgct cactgtaacc aggctttgct acatatgcat
    56821 ctgccttttc tcgcccctga gattttaccc ttcttgtata agtatccagt tgagtatcat
    56881 aagatctgta tgctgaagac cctaaaaact gtattccttc tgaattagct tgcttaacta
    56941 aatcttcaac tgcctttgca ggttctcctg ccatttgttt ttcttcctct gttgcttctt
    57001 caacaaaagg tatgtttacc ttagtaatat tttcaggagt atagtttttg ctaattccat
    57061 ttgtcttgtt taccaataat atatttgata tactatttga gtgattttta ttttgagaat
    57121 ttgcctcaac atcttgttct gaatcatgtt gagcagctac tattggatct ttatctttct
    57181 taagtaatct atctttagat ataaatacac ttataaatac tactaaaata atggctccta
    57241 ttaatttaaa ttttttcata attaaatctt ctcctttttg ttatgtttgc tataatgtta
    57301 attttagagt ataagactta agtattttat aagtaaatct taaataaatc ttaaatttaa
    57361 tatttttaaa ggatggtgat aaaatgtcta tacaaaaact tctaagtaaa gctcgtaagg
    57421 ctatacaaga ttttgatatg atacaagaaa atgataaaat tgcggttgga ctttctggtg
    57481 gaaaagatag tttaactcta cttcatatac ttaaaagtta tcaaagattt tctccacaaa
    57541 actttgaatt gatagccata acattaaata ctggaggtgt tgataattct ccactagata
    57601 aattgtgtaa agaaataaat gttccttttt atgaatttca aactgacata aaagaaattg
    57661 tatttgatat aagacaagaa aagaatcctt gtgcattgtg tgctaattta agaagaggtg
    57721 ctttgaacga taatgctaaa aaacttggat gtaataaagt agctcttggt catcataaag
    57781 atgatgcaat tgagacattt ttaatgtcta tgttttatga gggtagagta aattgttttt
    57841 ccccaaagac atacctagac agacaggatt taacagttat aagacctatg gtttacatag
    57901 aagagtatat gactaagaaa atttcaaaag attctaacta tcctattatc acaaatccct
    57961 gccctgcaaa tggtcatact aggagagagt acataaaaaa tcttattgct aatttaaata
    58021 aagagatgcc tgattttaaa agaaatgtat ttggtgcttt aaataattct gagaaattgt
    58081 ttatatggga taaagagaag ataaaaaatt ttaaataata attaaaattt ttaaataaaa
    58141 aagcaagatt gtattaattg taaataatct ttatataatc tacacaatct tactttttta
    58201 tttattatat gttattatct ttaatctata ttttaatagt tcttaatagt atttattttg
    58261 tctaatacat taattataac ttaaatattt tatatattaa tttaatctat taattcattc
    58321 aatctactgg tttaatccat ttttacagta tatacatcta catattataa aaagcaaaat
    58381 tatattttct aaatatttta tgttagatta taatattaaa ttctattatc tctggtatat
    58441 taaaaaatct aaatggtaac tttgtacttc caagacccga actcacatat aaatgatttc
    58501 catttatatc ataaaaccct ttagtatatt tccttccata tggaggagta actaatgctc
    58561 ccttaatagg taatcttatt tgaccaccat gactatgccc agatagaact aaattcattg
    58621 tatctttact aagcatatct actaaatctg gttcatgtgc taacaataaa ttaaaattct
    58681 tattatctat ttgactctcc agatatttta tattaggatt tccatttaat atttcatcaa
    58741 cacctaatat attaatatat ttatcatctt ttaattttat ttttttattt tcatttacta
    58801 aaagattgaa gttggaattt ttcataatct gcctataata cctaggaagt ttatacatat
    58861 aatcatgatt gccaaatact gaaaattttc ccaacctagc attcatactt gataaaatag
    58921 ttgtaatttc atctacactt ggatttttat tggagtaatc aattaaatca ccagtaaata
    58981 ctattatatc tggattcaaa gtgttaatct tattagctac ttttttgaga ttcttagttg
    59041 aataaaaact acctatttga gtatctgaaa tttgaactac ttttatcctt tcagatttac
    59101 tctcaattgt atcacttttt atcaatgttc cattttcact tatattatat tttttcacaa
    59161 ttaatagact cggctctatc gcaactgagt atataaaaat caaaataaat attaagataa
    59221 aaaattttaa aaaatactta atttttgagt tcaaaatatc tacctactta tttatttttt
    59281 taaactattt gcattacaga ctcaactttg ttcatttttc tgtcatgcat ttttggctta
    59341 tttgctgaaa accatttata tgttgctgta agaccttctt ttagtaacac atttggtata
    59401 taaagcccat gttcaataag cttatttata tcaagattaa agtctatatt tctaaatggg
    59461 aaatacgttc tttctctaaa ctctactttt tccatatcta catattttat tataggttct
    59521 ttgccaataa tttctccaca tgtatatata aggtcatccc aagaaattaa ctgtggattt
    59581 gtcacattat aggcctctct tacatgagga tttttcatta tgctttccaa tacctttacc
    59641 aaatccccaa tatatataaa ttgattacta acttgttttc cttttggtac tggtatcttt
    59701 tcattttttt ctattttttc aaagaaataa tactctctat ataaattatt attttcacca
    59761 tatatataag ttggtctaat aattatatat ggtatagggc tatttataat aaaatcttca
    59821 gcctcttttt tatttaaccc atattttccc cagttttcat tttcaccttt ctcattttct
    59881 tcttttatat ttcttccact atctttatat actgctcctg ctgacaatac tatatacttt
    59941 tttaagttat ccatacttat aaaatcaatc agattactta catcttcttt tgtatatgct
    60001 gtcatatcat atatgtaatc atattttctt cctgtgataa tgttttccat atcttttcga
    60061 acttttctat cacaaattag atgttcttta aaaccattat aatctatttc ctttttacca
    60121 tttgtaagta tatcaatttg atacccacat ttaatcaagc gcttagcaag tgcagaacct
    60181 atgaagtcgc ttccacccat aattaaaata gatttcattt taagtgcatc ctcctctttt
    60241 ttaaatgact tccttaacta taatatcata tttttaaata tgcgacacca ttttgtaata
    60301 tttttgtcta taaaagatat ttataacaaa atatctttta tagactctac attttcgaca
    60361 atataagtag gttcactttc actaagttct tcaaaagaac catacccata caacactcca
    60421 atagaatcta tacctatttt tttggctcca attatgtcat actttctatc tccaaccata
    60481 ataactttat ctttatcttt tacattacat aggtctagta catattgtat aacttcattt
    60541 ttattcactc ttgtaccatc aagattgcta cctgctatat atttaaaata tctatctata
    60601 tcaaaatatc taagtatagt ttctgcaaaa actgttggct tagaagttgc tactaataat
    60661 attttaccat ttttatagag catttctaat atctctttta tattctcata gattttattt
    60721 tcaaatatcc ctttatcagc aaaatattct ctatactttt caacagcttc tttagttttt
    60781 ttatcttcaa atttataata ctccttaaaa gtatcatgta atggtggtcc tataaattgg
    60841 tctaagtttt ccaaatcttc ttttatgcca aagctattta aagaatactg aatagacttt
    60901 gttattcctt ctttagggtc tgtaagtgtt ccatctaaat cgaataatac tatttcataa
    60961 ttttttttca tatttcagtc ctccatattt gtaaaaagta atatcttata tattaagttt
    61021 atctatttta aaaagctata gtaatagaaa tttctaatac tatagcttaa gtacccaatt
    61081 aaatcttata tatttatttt aaaaataaat ttaaacagct ctatttaact gttatagctg
    61141 aaactggaca tccttcttcg gcttctttac ataaatcttg taagtctgct ggtatttctc
    61201 cttcaattgc tactgaatgc ccttcatcgc tcatactaaa aacgtctgga catgttccta
    61261 tacatgctcc acaagctaca catacatcct tatcaacaaa tgctttcata ctaaaatacc
    61321 tcccctaaaa ttattatttt ttaattgttg tttccttaga tatttatcca atatatacca
    61381 atttaaacat atatttatta ttttatcact attgtttagt ttattatagt attttcgtat
    61441 ccatgtctat aaacttcact gcttttcatc catgatttta cagtatattg caatcttaat
    61501 tttttattta aaacaagttc attattattg tagtctttta taatatcatt acactcctcc
    61561 attgtgaata ttgtaaaatc accttctcca ccaatcttta ttctgccttt atttccatgt
    61621 aaatcgaaaa tctttgttgg gtattcagtt actttttcta tacacttttc taaggatatc
    61681 ccaagttcca taattttatt tataacactt aaaagagaat agtttacgtc tttcatgttt
    61741 tttatatgca tatcagtact tattaggtct ggctctaaac cttgctttat tgctttttta
    61801 aaaactttaa aatcaaattg gttcaatcca tgacatacat caaacaatac tcctctgttc
    61861 tttgcattta ttactgattt tcttaatgtg ccagattctt taaggattcc atttgtacta
    61921 tttccatata ctggtgtaac cacatctcct tttcccaaaa tattcattac ttcatctata
    61981 tatgatggat actctcctat atataccact aagggtaaat ttaatttttt ggcaacttgc
    62041 tttgatttct taattatatc tataccactt tcattagatt gaaatttact cgcttttact
    62101 tttataccct ttatcttttc tatatttttt tctacaactg actcaacctt gtcaaaattt
    62161 ataacatcta aagaagttga ttcattcaat atatttagtc cctcatttgt aacatttaaa
    62221 aaagaatata cttctgtttt acactcatcc atatactttt ttttaaaatc atagtagttt
    62281 aaaggaccag ctgtacctgc atcaaatata actgtagaac ctttttcaat tccaattgaa
    62341 tcagaatcca tacctgtagg taattttctc ttataacaat gggtgtgaac atctataaat
    62401 cctggggaaa tatacatatt atcggcattt attatctcga actctttatt tgtaagtaat
    62461 ttttcttttc ttattttctc tttaaggttc aatccaatag catatatttt cccatgtaaa
    62521 accaagatat ctcttttttc aaatttgata ccatctgttg gagatattat tgtcccattt
    62581 cttataagca aatttttcat ttattccaca tcctaattac aatataattt ctctaatagc
    62641 ttttcttaca taaagaaaat aagcctaaaa ataaaaatat tttagactta ttcactatat
    62701 tatactatat taattatatt aaattaaata aaatctttaa tttactttaa gaagcaatta
    62761 agtatctgtt gttcggcttc ttcttcagta agacctaatg tcatcatctt tagtatttga
    62821 tctcctgcaa tctttccaat agctgcttca tgtactaaaa gagcatctgg atgatttgca
    62881 gcaatttcag gtatagcact aatttgagaa tttcccataa ttattgaatc acattgaaca
    62941 tgtgcagaac aaagggaatc cccaataaca caaggattaa acttttgctt agaattatct
    63001 tgcccaactg tacgagaaac tacttgagca gaagagtttt ccccctctaa gataaccttc
    63061 atattagatt ctgcaacttg attaccatga gttaataatt tctcaaatac aactatttta
    63121 gctcctttac ctaggtttgc atccatttct cttttagttg aatctacacc cttaatttga
    63181 attgtttcca tttcacaata tgaatcctca tccatattga caatagtaac tggatttaaa
    63241 attctctttc cttctccatc accttcacca taatgttttt ctacatattt taggcgagct
    63301 cctttacgta agaaaaatct atgtatacca ttatgttcac tatcatcaca accacagtta
    63361 tgtataccac acccagcaat tataacaaca tctgcatttt ctccaatttc aaaatcatta
    63421 tataccatat cctgtacacc agtctctgtt actattactg gtatatgtac attttctcct
    63481 tttgtatttg gctttactat aatatctatt ccaggtttat cagttttagg aattatagtt
    63541 acattttcag ttgaatgtct ctctactcct tgcccatttt tacgtatatt gtatgctcct
    63601 aatggagttc cacgaagttc agcaactttt tctaaaagat ttaaatctat tttgttcatt
    63661 taatttgcct tcctttctaa ttaattcatg tctttcatta attcacaact tgaattaact
    63721 tgacacatga tttcaggtaa tatagcctct tttgttccat ggctttttac tgaaccatct
    63781 tgtagaacaa ttatttcatc agctaactct aatattcttt cttggtgaga aattatgatt
    63841 atagtctggt tagactcttc atgtattttc ttaaaacttt cattgagttt cccaaagctc
    63901 caaaggtcta tccctgcttc aggttcatca aatatagaaa cctttaaatc tctagcaaaa
    63961 agagttgcta tctcaattct tttcatttcc cctccagaaa gagagttatc aacttcacga
    64021 tttaaatagt cttttgaaca caatccaaca tctgtaagat attggcaaca tacatcagta
    64081 gacaatggtt ttccatgagc aagtgtaagt aaattttcaa ctgtcatacc ttttattctt
    64141 ggtggttgtt gaaatgcata tcctattcct ttttttgctc tttctgtaat actaagattt
    64201 gttatatctt ctccatctaa aattatctga ccactcgtag ctttttcaat tcccataata
    64261 agcttagcaa ttgtagattt tcctccacca tttggccctg tgataacaat aagttttccc
    64321 ctctcaaatg aaaggctaac atcatttatt attcctagtt cctcattgtt agactcaaca
    64381 ttaaaggaaa gattttttat ttcaagcata atacccctct tttctataaa atattacatt
    64441 aatatccatt cttttattat atcagtaatt tttatatatc agcaactcta agcagataaa
    64501 atgtatatta aataataaaa caaagttatt gaaaagtaaa aaagatgagt tttatgtaaa
    64561 ctcatctcag aaatttagta tatcaactta tatgtattta aaaattagcc ttctatatac
    64621 tctaatatta ttttttgatt atcttttaac ataatttctt cttttttaga ttctattcca
    64681 ttctttttta tcttcacctc agctacaaag gcatcattac cttcaaccat ttctaatagt
    64741 tcataagctt ttttacgacc tattttagtt gctatatctt ttagtactgc cattttctta
    64801 aacattttat cacctctact caaaatttac acttaatttt taattatata attctaatcc
    64861 tagactagtt tataataatt attgtctcta attatatcag atattgagta aatttaatac
    64921 tttgtaaaat tatttatcct ctctgaccaa ataatcattt ccatcttggt ctttaaaaga
    64981 aaacatcctt ccatatggca tctccattaa ttccccaacc tcaacctcgt tatttgccat
    65041 ttgttcataa gttccattaa tatctcttgt acttaaaatt attgatggat gagatacatt
    65101 tatctctttg ttttgtctca tcattagatt tttatcatac aacacaaatg ttgtaaattc
    65161 atttttactt ggtgctactt ccacccattt catatttgga cccatttcct tttcataagt
    65221 tactacaaag ttcaattttt ctgtccaaaa caactttgca tcctcttgat tatttacata
    65281 gagagttatt tttcctattt tatttatcat aacaataccc acctttatta ttaaataaat
    65341 acctaatatt ttttatattt aataatatac ccatatatat tattttctat agatatatat
    65401 ttctaatatt tatcgccaaa ttatgtaatt ctgctcaata tataatttat agtatccata
    65461 ctattatagg ctttatttat gtaatttaat caccttatat atcatactta taattatgat
    65521 aataactaca taaaaaatca cttctaaata gagttatcca tctaaaagtg atttcatata
    65581 ttctatgcaa ctttgtgttt tagccacttt gttcctctta atcttaaaca atataaagtc
    65641 cctctaacaa gccaatcagt atacatacct atccaaattc ctaacactcc aaattctaat
    65701 acaaccccca atacgtaacc tgtaaaaatt ctaaaaatcc acatacctat tactgcagtc
    65761 atcattgtat atctagtatc accggctccc tttagccctg ctgataatac aaaggatata
    65821 ggccatatta ccatagctaa acaatcactt cgaattaatt gtgttgagat gtcaattaca
    65881 ctagcatttt gagtatacat acttgaaatc caaggtgcaa ttggtataaa tatagctcct
    65941 actataaata aacaaattgt tgcaaattta gttaaatata ataaagtact cttagctcct
    66001 ttaatatctc cacgtcctat atactgtccc accaaggttg tagcagacaa agctaaagca
    66061 ttcccaatta catttattat ggatgcaatt gacattccta ttgcattgga tgcaattgct
    66121 gatgttccca tagtaactat aaatacttga acaatcaatt tacctgcatt aaaaagtagt
    66181 tgttcaactc cagctggtat tcctatatta aatatagact tttgagtttc aacatcaatc
    66241 ttaaatggaa atattttacc catagatata aattcgtttc ctttaaataa agctcttata
    66301 attactatac atccaattat tctagctata gcaatagata tagctgcacc tgttatgcca
    66361 taggatggtg tttgcaaatt tattcctaga ggatttaaat caataccata tataagtata
    66421 tatcctaatg ttacatttac tatattcata aacatagtca catacatagg tttctttaca
    66481 tctccacacc ctcttaaaag accatttgct atttgttgtg tcgcaataaa tggatacgta
    66541 aataatgtaa actctatgta taattttgca tttgatttta ccaattcttc tgcggaacca
    66601 taaaataaat taattacacc agttctaaaa acccacatca atattgttat tgtaaaggat
    66661 aatattactc cagacactag agcctgctta atagtttcat tcaccttttc accatctttt
    66721 tttcctattt gttgagctac aacaacagta gctccaacag atagtgcagc aaatacagat
    66781 ataaatatat tgtttattga atcaaccatt ccaactgcag atacagcttc ttccccaata
    66841 tgacctgcca tcattgtatt aaatactcct aaaagcatga caaacgtctg ctctatcatt
    66901 ataggaattg ctaactttat aacatttttc cttataaaca tccctattcc tcttttccct
    66961 ctttatataa atttaatttt taattagttt atcgaactgt aaattgacaa tgcaaagttt
    67021 agaattgtat tgttcgcatt ataatatact gctttcaaat aatataaatc ttgtttaata
    67081 tcatttgaac acagtatatc tttataatta tcttatgttt attatttttt atggatacca
    67141 catttaaaat atttattaat ttaatataaa tattttccgt tattattaaa agtagctata
    67201 tttcgttaat attgtatcac tatcgtgtag caataacaat aagtacattt tatatatttt
    67261 ttataatatt atttaatcat ccaaactttt accacttttt caatatataa aatattacac
    67321 tttagatgta acaaaatcgc atccatttaa atattgtatt ttatagacta attttaggat
    67381 gtgatacttt ggttaattta aatgcaaata atctaaatgt aaatatggtt tatcctattt
    67441 cttccacagc acccatacgt ttcagaagat atactgtttc atatttagaa aacatggaaa
    67501 atatttattt aactatagca acatactttg atacacttgg tttaggcaca atgaatatcg
    67561 acaaagttta tggtcaatgg gcttggttta ctgataatat atatgattta aatctatttg
    67621 tttttgtagg aaattatcct tatcaaattg ctaaaaacag atatgataca tttgtagata
    67681 ttttgccagt agctattact tctattgtca atggagatag aatattttta atttcaaatc
    67741 cttgtttgtt aaattcaaaa attacagtta gatttatatc tagtcatcct actcttaata
    67801 gaacagttga ttatggcaat attagagatt ttatttttta aaactgcaac atgatgtgct
    67861 attaaaaaat accctaaatt taatgatatt attaaataac tagaatattt ttaattggca
    67921 catcatgcct agctattata aattcataat attattttac ttaagataag tactgtattt
    67981 tatctacttg tatattttta tttacctagt ttctcagttc tatatcctat ttctttatta
    68041 gctagcttat aaccttcttg tatcatttct tctctcgtta atgcactccc taataaattg
    68101 tcaatactca gttgctttct atcactaaaa tacttatcca ataatgattc ctctatatct
    68161 tcctgatatg ccaaatttcc ttcatgcatt atatatttta catcaaaacc ttctttattt
    68221 aatacttttc ccaccaagat acttctatga catcttatag gttcttgcat agctcctaat
    68281 aaaactatcc tataacccat ttgacaaccc ttttttaatc tttctattcc gtttaaaaaa
    68341 atatcttctt ttgctacttt ctcaaaatct gcataacctt cttgcgtata tacatcttta
    68401 ttagttcttt tagctccaaa ttcttttccc atgtatatat ataaaaatcc ggaatttcta
    68461 agagattcgt taaatgcctc tttattgtat tgaatattat atttagaata tggagtttct
    68521 ctaatgtcaa ctacacaatt tatgtcatat ttttttatca tattaataag cttatcatat
    68581 ggataatttg aatgtcctat tgcgaatatt tccatataaa caccctccta ttctttgaaa
    68641 ttagtatcta tatatgtaga tacccgattt acttaaaatt aaataaagta ttactttaaa
    68701 tacccaatat
//
LOCUS       DAEPIN010000011        68577 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.011, whole genome shotgun sequence.
ACCESSION   DAEPIN010000011 DAEPIN010000000
VERSION     DAEPIN010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 68577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 68577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68577
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.011"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     regulatory      148..238
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            646..963
                     /gene="flgB"
                     /locus_tag="KR250_000890"
     CDS             646..963
                     /gene="flgB"
                     /locus_tag="KR250_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HBH0066855.1"
                     /translation="MNTYDIIKMGLDATNLRSQTIANNIANINTEGYKRHYITFEETL
                     NDAMPKIEEKTDNSKGLRVDGNNVDIEEEKVNQAMATLQYNALISFANGKIAMTKSII
                     SGR"
     gene            971..1375
                     /gene="flgC"
                     /locus_tag="KR250_000891"
     CDS             971..1375
                     /gene="flgC"
                     /locus_tag="KR250_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HBH0066856.1"
                     /translation="MSVFSGMRISASGLTAESLKMDIISSNVANINATRGPNGEAYRR
                     KVVSFEENYDNKLGMLGVKVTSVEDDKSPLKEVYDPSHPDAKPNGYVEYPNVNILVEM
                     TDLITASRAYDSNVDTLNAQKSMISKALEIGK"
     gene            1385..1702
                     /gene="fliE"
                     /locus_tag="KR250_000892"
     CDS             1385..1702
                     /gene="fliE"
                     /locus_tag="KR250_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HBH0066857.1"
                     /translation="MPITQINNLMQGAKLDNLIGEAKPLTLNNKEEKKNFEDVIIDTI
                     DKVNFQQVTADDMKEKFIKGEDVSMHEVMLIGQEAQLSMQYLIEVRNKIHDAYQEMNK
                     VQI"
     gene            1727..3277
                     /gene="fliF"
                     /locus_tag="KR250_000893"
     CDS             1727..3277
                     /gene="fliF"
                     /locus_tag="KR250_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
                     /protein_id="HBH0066858.1"
                     /translation="MITSFKNKIKEQVAKSRTKISSLKKGTKIALGIGVIAICLALVF
                     TAISASKNKYAVLFSELDAQDAADISAELESKKIDTKVKGSTIYVPRTEVDKLRLELA
                     PSLTGGSKGFALMDDSSSIGLTDEEFQIKKQRMIQGEIEKTIKSFPQVEDTRVHITFG
                     ESSVFEKEAVPGSAAVYLMLKPGTTLEKNQIKSVMALISGSVTNIPEKNVQVIDNKMN
                     LLSEGILEDENVDENNQPINSGISVDKNKASEQALNKQLEKSILEMLEPIFGKGKVKA
                     TVNADLSFDTAEKTEIKIDPDKVAIKETRSKNSSKSAANNVQGVDANMNNQGNNNGTN
                     TEDSLDESFEYETGKIETHTIVAPGELNRLTASVAIDGKIAKGVQADVEDIVNNAIGM
                     DGARGDTLSVVSMVFDPEGKKEAKQELEEIKKEGFKKNIIKAVIGVVAVIAVGAIGYL
                     IYKKRSEREDMDYEDEDYISKSDSVDRKDNPLLGDDPELTLEEAVKMYAEEKPDQVTE
                     IIKTWLNE"
     gene            3290..4360
                     /gene="fliG"
                     /locus_tag="KR250_000894"
     CDS             3290..4360
                     /gene="fliG"
                     /locus_tag="KR250_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HBH0066859.1"
                     /translation="MSTEITNVNAFDLSDINESDKNEKVEGEGLKKAAVLLMTLGPNV
                     SSDIIKSLPDKQVQKIGLEIANISSIASKERREILKEFVEINRAKDYVIEGGLEYARE
                     LLSNALGNVKANKLLEGISINTSTKPFKHIIKLDVKKILEILRNESAQTIAVLLAHME
                     PENGAEVLSYLDFDLQNEVAMRISSMGSISPEVIKVIESSLEDKLHSISGDDFNDYGG
                     VSTLVQILINSDRKTEKNILGTIEEQNPDLAAQIKSHIFVFEDIVKLDDISIQKVLKE
                     VTMKDLVYALKGVDDEISDAIYRNQSSRAADALKEEMEMVGVIKVTQVEASQQKIANI
                     VRKLEREGAITIVRNFGGDFVE"
     gene            4353..5084
                     /locus_tag="KR250_000895"
     CDS             4353..5084
                     /locus_tag="KR250_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HBH0066860.1"
                     /translation="MNNKIIKSKKALYKGVLDLTIIDKVRVGESNVNKENDCRFRDEV
                     IKEAIESAEIKKNEILEEAKINAQNIEKQAYEKGYSQGQKNGYEDGYKESYDEYTQKA
                     KDEADFIKNQANLILLQANEKMTEYIKEKNKEIVRLAINMASSVLKKHFEDEESMSDL
                     LKQIIQDYEGSSSFIVRCNSFYKDNIEKEFELFKEETSLKSSVFVIADDSIDKGNAII
                     QKENGRIIVGIDCAMEKLKEELLGE"
     gene            5088..6407
                     /gene="fliI"
                     /locus_tag="KR250_000896"
     CDS             5088..6407
                     /gene="fliI"
                     /locus_tag="KR250_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HBH0066861.1"
                     /translation="MYKTDISIDFDKINKKIENLELTISEGRVKKIIGLTVEVEGIKA
                     FVGELCVIYNQVNKPVNCEVVGFKDKEVILMALGELTLIAPGCKVISKGIPLSVMCSD
                     NLLGKVLDGLGNPIDNSDAVLGDRYNLNNEPPDPMKRKKIRNIMETGVRAIDAFTTCG
                     EGQRIGIFAGSGVGKSTTLGMIARNAKADVNVIALIGERGREVLDFIDKDLGEEGMKK
                     SVVVCATSDKAPLVRLKGALTATAIAEYFRDQGKKVILMMDSVTRFAMAQREVGVAIG
                     EPPAQKGYTPSVFAMLPKLMERTGTSDKGSITAFYTVLVDGDDFNEPIADTTRGILDG
                     HIVLSRDLANKNHYPSIDVLNSLSRLMNEIASKEDIKIASFARDMLAEYREAEDLINI
                     GAYASGTNKKIDEAIYYHEHIINFLKQGINEKSSFNETISSLRRVFE"
     gene            6438..6884
                     /gene="fliJ"
                     /locus_tag="KR250_000897"
     CDS             6438..6884
                     /gene="fliJ"
                     /locus_tag="KR250_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012815984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HBH0066862.1"
                     /translation="MELDKNFKFRLQKVLDLKVKDEEEIKMEFAKIQQKKIDIETNLE
                     NLESNYSKYSISKNNDSIQNQKITINYLLALNNSIMDLSEELDKSTNELEKARKQLIS
                     KQIERKSLEKLKEKKYGQYYKEENLKEQNTNDEFASMSYLRNRQVL"
     gene            6913..8118
                     /locus_tag="KR250_000898"
     CDS             6913..8118
                     /locus_tag="KR250_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HBH0066863.1"
                     /translation="MKIQADSYNIIKDIGDRKSSSKNKNIKNNKFEDNLYKATDHKKV
                     KDRREVSKQNISSDKPENSDNECHLDEDKSNHLEDENTYEKDCSKNNEALQQLLNLLK
                     DNYKKDKDINTILNNIEKLSISDELKQELKLNKNILNKIANQNSELNKANINNSNDIE
                     NLDIVKLRELLEDNANKDIEVITKTTENDTKNIDLYSFDSNRMDNLNSNKQKDDSSDI
                     LEKLAGVNGNKSGNFGQVINKNVDMNKIKNDNVNLKQIESNVMDDSIKAIKHMKTNDI
                     QELTIKLRPKELGDMNIQLLKDGESMRAVVTVFNKDVFDSINKNIADLKQHLELTNVN
                     IKDVSVQMHSDNTNTSDTFDKAFEQQNRQNNQENMSKKNTNDKHNNVVIEDDIKENTI
                     DDDRVDLLA"
     gene            8136..8744
                     /locus_tag="KR250_000899"
     CDS             8136..8744
                     /locus_tag="KR250_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlgD"
                     /protein_id="HBH0066864.1"
                     /translation="MYGIATALAGTQGNYRSNNLSQLERNINDIANKQVRSKGNQTVT
                     DNGTPIIGQGDDADKDLFMKILVAQMGNQDPMNPQDPTEYVSQLAQFATLEKMSAMNE
                     KLDVLLALSNSTLINSALSMATSLIGKEVEVSGEEKEDENIVGVVKSTYIKDGEVHLE
                     LEVDGSDKTVGVKYEKLLKIGEVDENSGEENDSSEHESPEEA"
     gene            8771..9754
                     /locus_tag="KR250_000900"
     CDS             8771..9754
                     /locus_tag="KR250_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HBH0066865.1"
                     /translation="MIKAMYSGVSGMKANQTKLDVIGNNVANAGTTSFKKSSARITDS
                     FYQTVLYASAPTAALGGTNLGQVGVGSKISSINKDMVQGNVQPTNRPSDLMIDGDGYL
                     PVVRQGTVMYTRDGSFNLDTGFKAADGALKNIKGGRLVNGDGYMLQGMIYDGEYKADT
                     GEFVKKGDAKGKEPIVIPLQSISPKDGTVQNVLEYNISKDGTVEFLLSDGQRTQFVYD
                     IPDGAKVGDTPAGIDKLKGTIQKVQTYAFQNPAGLDAEGGNLFKPSANSGDPQIAGAT
                     GTIIQGAIEASNVDIAEEFTEMIIASRSFQANSKIISTSDEILQEIINLKR"
     gene            9767..9970
                     /locus_tag="KR250_000901"
     CDS             9767..9970
                     /locus_tag="KR250_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FlbD family protein"
                     /protein_id="HBH0066866.1"
                     /translation="MIRVTDLNGRKYILNSDLIVRIDSVPESRILLTTGEKNLVKESV
                     DEIVDKVIKYKRKINVGYIRSEK"
     gene            9970..10788
                     /locus_tag="KR250_000902"
     CDS             9970..10788
                     /locus_tag="KR250_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HBH0066867.1"
                     /translation="MKKTDSLTFIGLIVSTVLVLVGAAKGSSSGLKNFFDVSSILITV
                     LGSFGALMITFTIDDIKLIKNALQYSFKTMSVSKLDLLEQFKTLSKKARKEGLLSIES
                     DVMGIEDPFMKKGLELCIDGLEINEIRSILELEIDSVEDTNNRYSKLFKTWGTFAPAF
                     GMLGTLIGLIQMLADLTSPDLIASGMGKALITTFYGSLLANIALNPIAYNIDEKTEKE
                     IYVKEMMLEGIISIQSGESSIVVEERLATYLSNNEKLEIMKSNKNTERAMSNGA"
     gene            10778..11473
                     /locus_tag="KR250_000903"
     CDS             10778..11473
                     /locus_tag="KR250_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HBH0066868.1"
                     /translation="MARKKNKKKDEINPDAWLATYADTITLILTFFVLLYATSVVDME
                     KFKNIADALKGQFSGTSIFSTGSSTSEKSPIDSVLVDKNDKSNLEQDLKDKVNNSNLQ
                     NSVTVKDDSRGILLELDDSILFDSGISELKVDSKATLNKVYDMIKMMKNKIVIEGHTD
                     NVPVKSSTHESNWDLSSSRAVSVVRYFVEEKGMDPRLFSATGCGEYQPLVSNETAKGR
                     AENRRVNILITID"
     gene            11485..11928
                     /locus_tag="KR250_000904"
     CDS             11485..11928
                     /locus_tag="KR250_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HBH0066869.1"
                     /translation="MFKNKKIIIIILILIIFSAGAGFFILGEGGNKQVDKIADLFIPT
                     GKGKDEVKTENLDLEEMVLKLADEGVRYLKVSITVSYDSSYKDIEKNTPLIRDNIINC
                     FMAKRADDFTADNLKNIKEEIKNTLNIQLGENVIQNIYFTNIVVQ"
     gene            11943..12317
                     /locus_tag="KR250_000905"
     CDS             11943..12317
                     /locus_tag="KR250_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliZ"
                     /protein_id="HBH0066870.1"
                     /translation="MILYLLKILICIPIILILIVLSLKISQGTLLKKYNDKYVKILDI
                     VNISKNNSIIILKIGEEGCIMASSSSSMSKIKDLTKEEILKIENDIKERNFKIQDFKM
                     ERIKKKYDFSRYIGKFTKKEDK"
     gene            12318..12983
                     /gene="fliP"
                     /locus_tag="KR250_000906"
     CDS             12318..12983
                     /gene="fliP"
                     /locus_tag="KR250_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425215.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HBH0066871.1"
                     /translation="MELLSKLPLADVPYTSSMQLFLFLTALMFLPFVMFMMTSFVRIV
                     ISLSFLKSALGAQQAIPSQILVGLAIVLTIFIMRPVLNEINEKALQPYMKEEVTMEEA
                     MKEAEGPIKEFLLTQTRQTDLDLFVEQAGLKEKKLTRENIPLSVVVPAFAISELKTAF
                     QIGFLIYIPFLIIDLVVASVLMSMGMFMLPPVMISLPFKLLLFVMVDGWNLIVKTLIL
                     GFG"
     gene            12996..13265
                     /gene="fliQ"
                     /locus_tag="KR250_000907"
     CDS             12996..13265
                     /gene="fliQ"
                     /locus_tag="KR250_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HBH0066872.1"
                     /translation="MSEFMIMSVLKNGMFTGAMIAAPVLIVSLVVGLLISVFQATTQI
                     QEQTLTFVPKLIAILLVVAIGGSWMLHTLISFTSEIFNMISLITK"
     gene            13277..15091
                     /locus_tag="KR250_000908"
     CDS             13277..15091
                     /locus_tag="KR250_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused FliR family export protein/FlhB family
                     type III secretion system protein"
                     /protein_id="HBH0066873.1"
                     /translation="MIFVFIRIVAFFGTLRILFPAGTPALFKSLFAIIISILISSTMQ
                     IEYATNIDNIVLFTLYGVNETITGIMLGYITNLCFYSIRMAGSMMDQQMGLSMINMFD
                     PNSMTQTTLIDNLMNWTALMIFFSMDGHHVLIRGIRYSFELIPIGKPFVDNNIDYIIN
                     IFVQCFLTGFKIAIPIVLCLLMADFILGLISRSVPQLNVMIVGMPLKILVGIALFIIS
                     IPLIVNQISHLLSQIPKMYEGTFALAPMFFMGSADKTEEATPKKKGEQRKKGNIAKSR
                     ELPVAMTLLAFTLLVPTLFSYVVDTLKSSLNYFLSLDFYMNINYSNLEKLVIAGLMDF
                     FKIFLPIAIPFLVLGIIANLFQVGILFTGETLKPNLSKLNPISGFKNMFSMRSLSTLI
                     KDTAIISILAYIGYTFFQDNYLDILKLGNIYLPTLMYTVKDLVYSILSKICVAMIAIA
                     VADYVYQRYSHKKQLRMTKQEVKDEYKNSEGDPEVKAKIKQKQRQISSQRTMQAVPSA
                     TVIVTNPTHLSIAVRYEKGKDQAPVVVAKGADYLAFKIREIAKGNNIPIIENKPIARL
                     LYKQVEIDQEIPEDMYQAFAEILVAVYKIKNRYKVPKR"
     gene            15100..17175
                     /gene="flhA"
                     /locus_tag="KR250_000909"
     CDS             15100..17175
                     /gene="flhA"
                     /locus_tag="KR250_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HBH0066874.1"
                     /translation="MNKFNLKENMDVIVAFGVVGIVLMMIVPLPTFILDILLAINISL
                     SVLILLMTLFTTNVLDLSIFPTVLLVTTLFRLGLNLSSTRLILTQGYAGEVIEAFGSF
                     VVGGNYVVGIIIFLIILIVQFVVITNGSGRVSEVTARFTLDAMPGKQMSIDADLNAGV
                     IDDKTARKRRKELQQEADFYGSMDGANKFVKGDAIAGLIVTAINIIGGIVIGAVMLNM
                     TLMGAAQTYVRLTIGDGLVSQIPALLISTSAGIIVTRASTDENFGDLVGKQLTAIPKA
                     VMMTGCVLIVLGMMPGLPKPAFFSLGIGAIAAGYFLGREKKDTLEDLEFEKPDMVNPD
                     KNEVEDVTNLINVESIEVEIGYGLISLADESAGGDLLQRIASIRRQCAIDMGIVVQPI
                     RIRDNLQLNPNQYNIKIKGNVVGGYEIMPNMVLCMDPMNQGFSIFGIKTIEPTFGIEA
                     LWIESDKVEDAELKGYTVVDSSTILITHLLDIIKSNAHELLGRQEVKTIIDAARENYS
                     AVVDELIPDLMTLGEIQKVLKNLLREKVNIKDRVTILETLADQSRNTKDIELLTEYVR
                     IALARSICNNLVDDDKTIVVATLSPETEELVSNNLQRSVNGTYPAVDPENTNKIFESI
                     QEVMSNVYFNHNIPVIVVSPKIRAPFRKLVEIVYPNLTVLSLNEIPNDIKIKAEGVVS
                     IYDDEKVYS"
     gene            17150..18166
                     /gene="flhF"
                     /locus_tag="KR250_000910"
     CDS             17150..18166
                     /gene="flhF"
                     /locus_tag="KR250_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HBH0066875.1"
                     /translation="MMTKKYTANTIQDAMNLAKMELGDNITLIDKKEVRKSGIQGIFS
                     KKDIELTIGWEKRENVEQKDLKREIEQLKSIINNMGFDNKNDNDIDKICKNLLSLELN
                     EEIVESIRIDLQEMKFNGIDTSKNLVEILKKKIKIENQVINGKIALVGPPGVGKTTTI
                     AKLAAKLVFEENKKVGVITIDTYRIGAVEQLKIYTDIMNIPFKGVISPDEMELALDEM
                     KDCDVVLIDTTGRGYKNSMQILEIKNLIDKAETDNIHLVLNCTTRESDTKAIIDSYRN
                     VNFKSLIITKLDETITYGSIFNIMNYAQKPISYITTGQNVPDDIIKPNEDKIIRLLLG
                     VESI"
     gene            18163..19041
                     /locus_tag="KR250_000911"
     CDS             18163..19041
                     /locus_tag="KR250_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HBH0066876.1"
                     /translation="MIDQAQILRKIAMEKRGLDEFIVENENVPKIITIASGKGGVGKS
                     NLATNLSICLTKLNKKVLILDADIGMSNIDIIMGVNVKGTIIDVINGEKNIEDIISQT
                     KYGVNIISGGSALNHIEDFTEAQRNKFIHSIEQIHDVDFIIIDTGAGMSKSLLSFIYC
                     STEFFLITTPEPTSLTDAYSLLKAISNFGIKRSANIIINRVIDLEEAKSTYKRIKMVV
                     DKFLNLNLELYGYIIDDKKVSLCVKKQIPFILEYPTSIASKCIIAIAKRMVSQSREDI
                     VDNKSFLRKMFSIFKS"
     gene            19055..19756
                     /locus_tag="KR250_000912"
     CDS             19055..19756
                     /locus_tag="KR250_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HBH0066877.1"
                     /translation="MNREELIKENMPLVKSIARKFFIPGKGFEYQDLVNSGVIGLIDA
                     INKFDAEKGAKFSSYSYIKIKSAILDEIRNQSPISKHNLTKVNKYNRVVEKLQSKLLR
                     EPTSYEIAKELKVSEKELHDIESNIDMLNIVSLNYVIFEDTNETVQDVISDREEEAPE
                     NIIEEEEKLEILSKAISNLNEREKLILSLYYYEDLNLKEIGKVLGVSESRVSQLHRKS
                     IRNLRNKIKELKYSI"
     gene            19772..20194
                     /locus_tag="KR250_000913"
     CDS             19772..20194
                     /locus_tag="KR250_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein"
                     /protein_id="HBH0066878.1"
                     /translation="MIQIILIVVSILIIVTIVINLHKESKNKDNRYFDKIFEQEYEAN
                     DRLIIAEKRRFFLDKVLAEKRINSHIKVYENYNLIKANEERKENIFIESKKEVRQENE
                     LSLKVKHLISSGYDDVEICKMLDIGKGELLLIRSCYKI"
     gene            20179..20364
                     /locus_tag="KR250_000914"
     CDS             20179..20364
                     /locus_tag="KR250_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein"
                     /protein_id="HBH0066879.1"
                     /translation="MLQNIRILLCDKKVFLGIGIGILISTLCIMLFSNNDMSKAEIEK
                     KARALGMKYTSEMKVLE"
     gene            20405..21178
                     /gene="flgG"
                     /locus_tag="KR250_000915"
     CDS             20405..21178
                     /gene="flgG"
                     /locus_tag="KR250_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgG"
                     /protein_id="HBH0066880.1"
                     /translation="MIRGLYTAVSAMITNQQKQNVVVNNLANMDTNGYKSKQLLTKSF
                     DELKLEGYNNYANGTKQKQIIGDISPGISMDETITNFNQGPIKTTDNKMDVAIQGKGF
                     FLVSDAAGNQFYTRNGNFRTDNQGDLITSEGYYVLGTNTATGAVGHINVGNQKFEVAK
                     DNTISLDGNAMYKFNIVDFNNYNNLKKEGDNLYSGGGAMKVNNALTIQSAIESSNVDM
                     VSEINNMMTISREYEANQKIIQAMDSKLAKIASEIGSVR"
     gene            21199..21960
                     /gene="flgG"
                     /locus_tag="KR250_000916"
     CDS             21199..21960
                     /gene="flgG"
                     /locus_tag="KR250_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgG"
                     /protein_id="HBH0066881.1"
                     /translation="MYTMFYTSKTALSANQSKLDIISNNIANSDSTGYKKINMEYSDL
                     VDEVLNRPSYPTNGNDISTGTGAKATAPMRNYEQGALAPTDSKSNLAIDGEGFFRVIR
                     KDGTYAYTRNGGFNVDALGKIVDDNGNILDVQFDAGYNYNNTNIDSTNLTVSRNGELF
                     VDNKKIGKINLYQPIGTQNFISEGDSLFVATDAAQIKQVEKVDIVQGYRERSNVSLQE
                     EFVDLIATQRAFQMNSKGIKTADEMWQIANNLRAK"
     gene            21983..22864
                     /locus_tag="KR250_000917"
     CDS             21983..22864
                     /locus_tag="KR250_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HBH0066882.1"
                     /translation="MNDMKEYDFGKPQSYSRENLTCLNFLLAEFCKKYKNYIIYELKC
                     NSSMVVDSIDQVNYQTFLDRVTSSCVVVQNAMEPVMKNFSFRIDRSVADMWIDITSGG
                     TGVVKDNDRELTELDKKVLLHLMDDLVRNMHLFEGFENIQVLDTHTHINLPQLCSPTA
                     PVCVVDLKVLNDNEYIGSVSLCFPYNGLEALLESISVIEFFDNDVGDDSEEFTQKIYN
                     SVSNIELSVIAEIGKVSINVEDLLKLEVGDVIVTNKKINDYIDIFVENSKSYTATPGF
                     ISSKKGVKINDAVGKEV"
     gene            22867..23241
                     /locus_tag="KR250_000918"
     CDS             22867..23241
                     /locus_tag="KR250_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HBH0066883.1"
                     /translation="MDNSNLISDEEAKMLLNDKVESEVDGDDELALDVSEVNRNNIQR
                     ILDLKLELSVVIGRTKMTLRDILNLHKGSLIELDTLADQDVEILIDNKVLAYGKVVVV
                     DLNFGVKITSIVSEEDMVKSLV"
     gene            23273..24676
                     /locus_tag="KR250_000919"
     CDS             23273..24676
                     /locus_tag="KR250_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0066884.1"
                     /translation="MVLILNLNDIKKDILKLNKKDSCSLYNESINNINNNCISTGLKF
                     LKQARDLNPDDVDILNLTGLVNLLKCNFDDAVESFYKSSLCEKTALCEKYINMLTSEE
                     FLIFFEKYNQLIRLINKGMYKEAIQGFKLITDQYCDLIEPYELLALLYDKEEEYIKFD
                     ECLEVLKIIDKENYLLNQENNTLYMYNSANIQTKSNIKVDDYKNEFLHKSSSNHKIND
                     YNDVTKEKSSSKNQKSSDSNKKKSIKKNKYLYIAIILGILLVGQSIYSSYKMNNLTDK
                     ISKNNETKNEESIDSNLTKKENNIEENEDMSKASNINKSNKNINKEDDVKSKKNTVEN
                     EDDKDSDLFTEDELMSKANTLKSEKKTKSSIQLYKKVADIGENKGNTSEATYQVAILS
                     EKLKDYKTAEEYYKMYVENYSEKDAYFDESYYNLSMMYYNNGDLKNSKLTLKKLVNKV
                     PNSMYNNSKVKEILKEE"
     gene            24792..26729
                     /gene="htpG"
                     /locus_tag="KR250_000920"
     CDS             24792..26729
                     /gene="htpG"
                     /locus_tag="KR250_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HBH0066885.1"
                     /translation="MEFEKGSISIHTENIFPIIKKWLYSDKDIFIRELISNGCDAVSK
                     HKRLVSLGEISENKSSDYKITVSVNKGEGTLKFIDNGIGMTEEEIKKYINQVAFSGAE
                     DFFNKYKDKMEESNDIIGHFGLGFYSAFMVSKKVQIDTLSYTEGATPVRWISEGGTEY
                     EISESDARNDRGTTITLFIDDDSKEFLDEFTVRGIINKYCSFLPVEIYLEDVERLERE
                     AKEAEEKAKKQKEDGKEEIVDAKVIEPLNDTNPLWLKSPKDCTDEEYKEFYRKVFNVF
                     DEPLFWIHLNVDYPFNLKGILYFPKLKNEFELTEGKVKLYNNQVFVADNIKEVIPEFL
                     LLLKGVIDCPDLPLNVSRSFLQNDRDVSKISKHIIKKVADKLKSLCKNEREEYNKFWD
                     DIQIFIKYGCLKDESFYEKVKECILFKTIDDEYITLQDYLEKCKDKHENKVFYVSDKE
                     QQSQYIKLFKEYDLSAVVLNSSIDTHFISFMEYKENGVKFNRIDADLSDVLKDKNENK
                     DSEENKEEIAKIEGLFKEAVGERVKNYSVEGLKNEDTPAMVLVSEQSIRMAEMQSRFA
                     GMDLGMNFEEEKTLVINENSPIIKKLVSLKDDEEKKDKITLICNQIADLALLSNKELK
                     PDELDSFVQRSNKLMSLLIEL"
     gene            27116..28246
                     /locus_tag="KR250_000921"
     CDS             27116..28246
                     /locus_tag="KR250_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HBH0066886.1"
                     /translation="MNFNYNLPVNILFGRGRINDVGKEVSKYGKKVLIVTGKNSTKKT
                     GLLDKTIDLLKDSKIEYEVFDRVEQNPLITTIYSGVEIIKSTGCDCVLGLGGGSIMDA
                     AKSIAFSYKNPGDLNDYIFGIRQGEEALPIILVPTTSGTGSEGNCFAVLTNSETKDKK
                     SIKKNSMYAKASIIDPELMVTMPKHIIASVGFDALAHNMEAYLSNGRNPLADVQAIYG
                     IELISENLIKVYNDVNDLEAWEKITLASTIGGMTIGTAGTALPHGMEHPVSGLRDVMH
                     GKGLAALTPTIIESSWDSDIERFGNISKVLGGTCAEDCSDAIRNFLKKIDLDIKLSDL
                     GIEKSDIDWLTDNCMKISVANIKRHPKYFNKEQIKEMYHKSL"
     gene            28473..29495
                     /gene="splB"
                     /locus_tag="KR250_000922"
     CDS             28473..29495
                     /gene="splB"
                     /locus_tag="KR250_000922"
                     /EC_number="4.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore photoproduct lyase"
                     /protein_id="HBH0066887.1"
                     /translation="MFNPKRIIFEKGTLDTDVGKKIYNKFRDNKDVEIINISSNRIKE
                     HIPGENLYEQYRSGKQTMVVGIRKSLKFQTCKPSANYQLPLVSGCMGRCEYCYLNTQL
                     GDKPFIRVFVNVDEILEQTKKYIEDRKPQVTIFEGAATSDPVPVEPYTNSLKKTIEFF
                     GREEKGRFRFVTKYSDVDSLLDAEHNNHTEIRFSINTPRIINEHEHYTASANKRIESA
                     VKLARAGYQIGFIIAPVFLYEGWKEEYKNLIISIKEALPKDFDKKIIFEVISHRYTTK
                     AKNRILEIYPDTLLPMENEDRKFKYGQFGYGKYVYDKEQLDDMKEFFLKEINGVFKNS
                     LIKYII"
     gene            29677..30588
                     /locus_tag="KR250_000923"
     CDS             29677..30588
                     /locus_tag="KR250_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBH0066888.1"
                     /translation="MHAWESIQITLDLIEANLSEEISINELANKANLSPFYYQRLFKR
                     LVNKTVMEYIKLRRLARASEYLAEHKNRILDVALNFGFASHETFTRSFKKAYGLTPEK
                     YRANPVKLNHFIKPELILNYAMVDEGVPLIADDIVIEVNRKTLSNPRTFVGIEIEVPI
                     CQLVGGETTGIAIVEELWMKLGSQRHNIPNQIPGGNEFAALYMGNAKEGNCMYMAGVE
                     VESGTSVEGYSTFELPAKEYLVCGFEAENFNELVNSAVFKADKFMERWMKKHNLTTTD
                     FAIEMYYPTTPEAAYLEHWIVPVPIEQ"
     gene            30603..31019
                     /locus_tag="KR250_000924"
     CDS             30603..31019
                     /locus_tag="KR250_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HBH0066889.1"
                     /translation="MNWQDAFFQDTKPTFEQINEFINNPLWDKLNKTLVSTYNVEPKL
                     EYSKCSMQRGWNVKYKKRGKSLCTLYPQEGYFKALVIVSESNRVEVDLFINTCCDYIK
                     KIYNEVNFFNGSKWLMIQVDSLLVLNDMLELIKFRA"
     gene            complement(31119..31511)
                     /locus_tag="KR250_000925"
     CDS             complement(31119..31511)
                     /locus_tag="KR250_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0066890.1"
                     /translation="MSINTEKNITKNVEELTCPIRYALDIVGGKWKLPIICMLAVENP
                     IRYSSIKRKLDGITNTMLAQSLKDLESTGIVHRKQYNEIPPKVEYTLTSKGKSIVPIL
                     QQFANWGATNMQEKNTCGLSCKECRKIK"
     gene            31652..32047
                     /locus_tag="KR250_000926"
     CDS             31652..32047
                     /locus_tag="KR250_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HBH0066891.1"
                     /translation="MKALEFLNKARVYYLATVDENNQAHVRPLGFVMEYNGKLTFCTS
                     NQKDMFKQLIFNPKVELCCIDQNLNTLRILGEAVFVTSKETQAKALEIMPSLGQMYSV
                     GDGKFEIFSIDKAKVSCCSMSGKKIDVEL"
     gene            32450..32656
                     /locus_tag="KR250_000927"
     CDS             32450..32656
                     /locus_tag="KR250_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0066892.1"
                     /translation="MSKYSSLWEYFKKNNSQSFKLTFEEIKDIAGIEIDHSFLKYKKE
                     LNEYGYQVGKISLKEKTVIFDKIG"
     gene            complement(32813..33544)
                     /locus_tag="KR250_000928"
     CDS             complement(32813..33544)
                     /locus_tag="KR250_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0066893.1"
                     /translation="MLSKKNVSLIILVIILSLLTAIFIFNNKNTNNKLKEVTKNNEEI
                     VPKNLEGLSIEQLIEIRDKAIDKNLEGLREFGEDSVKKRKDSILNFEFGLVAFVNKNY
                     SNLTEKEKLIRQINIVNYENSINDIMDPSGSFFELANQNYQVNEFLLKNGKDYKEWSY
                     EGLDFLIDHLEELCKFVDFDVKDVIDIIDPTIKTDLSEEQKKSLNDKLKKISSSDTLS
                     EEVKKEIKNWENNLNNLNMNKNNSN"
     gene            complement(34382..35125)
                     /locus_tag="KR250_000929"
     CDS             complement(34382..35125)
                     /locus_tag="KR250_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HBH0066894.1"
                     /translation="MNFLSDLHTHSIVSGHGYSTLLENINYCKENGIKILGTSEHGPK
                     MPGSPHRWYFHNIQNIPRIINNIIILRGCEANILDIKGNIDLEPFVIPRLDYLILSFH
                     EAVFSPNTLENNTKALINAINKHDNIEILGHLGNPNYPIDYELIIKLAIEKNILIEIN
                     NCSIKGVSRNGSSDNCKYIATLCKKYGAKIILTSDAHICFDIGNYEYSENILKEINFP
                     DELIMNYPKKLINHFHQKGKLLDVDYTNI"
     gene            35291..37858
                     /locus_tag="KR250_000930"
     CDS             35291..37858
                     /locus_tag="KR250_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBH0066895.1"
                     /translation="MTQKKLSRKEKVLICLKRLTEEHLNFNNGIRFGFDAEYIATEVG
                     INRSNASKELNQLIKESIIIKIKGKPVQYLYKESIESILGKKLNKGIFDNINSINEYS
                     ENDSIKDEYILESIKKENNIFNLVGCDGSLRSQIEQAKAAVIYPPKGLSTLIIGPTGV
                     GKSIFAEYMYKYSLTIKEDSDNAPFIIFNCADYSDNQQLLLAQLFGYVKGAFTGADKD
                     KYGIVHEANNGVLFLDEVHRLSAEGQEMLFLLMDKGIYRRLGEANKVHECRVMIIAAT
                     TEDPETAMLETFLRRIPVTIKIPSLEERGFQERMKLICYFFKEESVRIGVKLKVSKEV
                     IKAFILYKCKGNIGQLKSDIQLICARAFLDYMTYKRECVYVRLSLLPNNIRESLYGNN
                     RKEEFVQNFSFIGKDDIIFLPEEKDFESENLLIENKKYDLDFYGMIKETWNKLQQEGI
                     KESQIRSVIDGDIQKYSYNLMNTFIYNSSNQTAYNNIVNDSIATTVKYILQKHDKWSK
                     REGLDKLIKAIALHIQNLIERIKIGNIVKHPNKDEIMKERAYEYNIAKEILSNMSLMY
                     GVEFPEDEAIFLATFLYLSYVGLNEESIAILVIMHGESTASSMVNVANTLLDCNHAVA
                     INMGLEDRVQDILVQAVEIANKIDKGKGILILTDMGSILTFAKVIRDATGKEVKAIDM
                     VSTPIVIEATRKALTPEMTLEKLYFNIIESIKKHYGDMEVTYAEKDNGGRYFDRLLID
                     NISKTLTFLNGEKAYFILKEVINRISEHYSLVIQDELLVKFIFHCSCMIERVIIKESL
                     VYKSYEERISRNKELYLVIKESFKLVEETFDIIISDMEYANILDIFESQYDTDKFKS"
     gene            38088..38492
                     /locus_tag="KR250_000931"
     CDS             38088..38492
                     /locus_tag="KR250_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBH0066896.1"
                     /translation="MIGIIVMTHGSFSEEIIKSCELIAGPAERTAAIKLNRNDNIEDL
                     NKNFVEKLNELDEGDGVLVLADLLGGSPSNVASLNLKKGGKFHALTGVNLPMLLEALI
                     NREGKSLEELAEACIEAGQTGINNINKVLSSM"
     gene            38516..39013
                     /locus_tag="KR250_000932"
     CDS             38516..39013
                     /locus_tag="KR250_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBH0066897.1"
                     /translation="MSLVKYCRCDDRLIHGQVIYKWVKHLGVKKIVVVDDETTNDVIA
                     KGLIKMAAPKNIDLSILTVSESRRYFYNNQADDNVFVLIKNLDTANRMIEEGINIKKL
                     IVGRIPTGIGKKKISQNVYINKKEFLLIDEFIKKNINVSIQMVPDEEEVELNQNINRY
                     KGEFI"
     gene            39016..39417
                     /locus_tag="KR250_000933"
     CDS             39016..39417
                     /locus_tag="KR250_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HBH0066898.1"
                     /translation="MNKYKVIILCHGTWGKVLVEETNKNFGLSNQYEVLSLSSEKDVS
                     VFMKEVEEVVDKEDVKIIISDLYGGSTSSVAIAVAIRRGIDAINGLSLQTILIVDEEL
                     SKEDKLHTLPERIVKKNNNLCVDLIKEFKKI"
     gene            39435..39923
                     /locus_tag="KR250_000934"
     CDS             39435..39923
                     /locus_tag="KR250_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBH0066899.1"
                     /translation="MAKITLVRVDHRLIHGQVITKWVKIAQAQKIIIVDDFLGQDEFM
                     ADIYKMAAPSGVEVVILTAEDAGQAFQNNTLGDKNIFILFKNVDMANKAYKAGLKYEK
                     IQLGGIPNEAGKKMVFTAVSLGNEDVEQLNELNENGVEIVLQVIPEESSMTYENALKK
                     FK"
     gene            39950..40705
                     /locus_tag="KR250_000935"
     CDS             39950..40705
                     /locus_tag="KR250_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBH0066900.1"
                     /translation="MESVLMLAIVTGLWYWFAAGLAGYTLFSTLKSPLFIGFSLGLLW
                     GDVTTGMIVGASIEMVYLGMVAAGGNIPSDKCLAALIAIPVALQTGVNAEVAVSIAVP
                     LGVIGVLVNNLRRTGNAVLVHKADKYAEEGNTKGIWRCATLYSLIFGFVLRFPIVFVC
                     NFFGADLVQSLLDVIPQWLMNGLTVMGGILPALGFATTIFTIGKNKFLPMFIIGFFMV
                     QYFEISITAAAIFGVCIALLITFMKEDKRVGEV"
     gene            40705..41568
                     /locus_tag="KR250_000936"
     CDS             40705..41568
                     /locus_tag="KR250_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBH0066901.1"
                     /translation="MQEIKNKEVKNGQGPTEVNKTRVLTKKDVTKTYLRWWWTAELSN
                     SFERMQALAVCASFTPALEKLYKRKEDLVDALKRHLQFFNTQAIWGGLIHGTVLAMEE
                     EKATEGKIPGEVISGVKNGLMGPLAGIGDTLDFGTFQTIFLALGASFGAEGSVIGAFF
                     PIMFSILLFCEGYYLFHLGYSLGRDSIKKILSGGIVNKIIDGASILGMFMMGALSATT
                     VKLSTPLSFDIGGKAIVVQDTLNMIAPGLLPLGVVFFVYWGMKYKKWTITKLLVILVV
                     LALVGSFIGIF"
     gene            41615..42094
                     /locus_tag="KR250_000937"
     CDS             41615..42094
                     /locus_tag="KR250_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HBH0066902.1"
                     /translation="MSEKMYTEKEYLEGIRGATGERAVWFYLLMKEAEKLGANPDDIC
                     KEAIYGFGKMRGQKYNVADTPGKMAEMLYNSKGQKVFEMELVENTDENGVLKFHHCPL
                     DAAWKEYGLTKEERKEICRLACYGDYGRVDCAQGVKLDFAQKCAHDDEVCELVFTKK"
     gene            42159..43466
                     /locus_tag="KR250_000938"
     CDS             42159..43466
                     /locus_tag="KR250_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBH0066903.1"
                     /translation="MDISRSVDNSLNRTIEFLKELIKIDSQQGEPISQCPFGIGPKKS
                     LDKTLDYCASLGFSVKNIDNYIGYAEIGEGEELIGIPMHLDIVPPGEGWSVDPFSGAV
                     IDNIIYGRGVIDNKGAVSMLIHVLKNIEDMYPTINKRIRLIFGTNEETGMKCIKYYLD
                     KGEEIPSMGFTPDAMYPVVNGEKGRVHIRIEKEIKIDKSKPYIIVSGGTKENVVPSHC
                     TAKIINGIISELTTKGVAVHASNPEKGENAISKMVIKIVEDNMDFQHREDIELVSKYL
                     CSDYYGDALGINQYDEVFKNTTLNLGILKVNEEKIVCELDIRYGKNIVLNNIIDRFKK
                     VFCNGWKIEVIVHKDLHYVDESNLVLKKLLEAYEEVTDENGYTIAMGGGTYASWFKDM
                     VAFGPKFLAYKTGGHGVDERVPINHIRKNMEIYTLALIKLLEL"
     gene            43776..43916
                     /locus_tag="KR250_000939"
     CDS             43776..43916
                     /locus_tag="KR250_000939"
                     /inference="COORDINATES: protein motif:HMM:NF021468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2185 domain-containing protein"
                     /protein_id="HBH0066904.1"
                     /translation="MNGEGCILSNKITKECYKVGYMIREKSIDIYPDSGWGFMTWDEN
                     GE"
     gene            44426..45520
                     /locus_tag="KR250_000940"
     CDS             44426..45520
                     /locus_tag="KR250_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0066905.1"
                     /translation="MNELNIAKTLILKRKEKGITQDELANYIGVSKASVSKWETGQSY
                     PDITFLPQLATYFNITVDELICYEPQMMKEDINKLYNKLCKDFTVKPFDEVMDEIREI
                     IKKYYSCFPLIFRMGLLIVNHYDIVDEKKRELLISEALEIFIRIQETCNDIDICRQAK
                     SMEATCYILLNQPIQVIDLLQNSNFPMINESILLAQGQMMNGQMDEARETFQLGAYQN
                     LISLVQNLVGILQNADKLQMKEIERRILAISDIFELDTLSPAIMLSSYLTQAQINLIH
                     GDNEGAIKSLRKYVDLATRDIYPIIIHGDDFFNKLDRWISEIGTGITRDDTIVKAGIV
                     AAIKNNPMFSVLSENKEYKFLIEKLSLLEE"
     gene            45525..45725
                     /locus_tag="KR250_000941"
     CDS             45525..45725
                     /locus_tag="KR250_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLDc_N domain-containing protein"
                     /protein_id="HBH0066906.1"
                     /translation="MENLMEYLPLLVPVIILDLILIITALVHVLRHPNYKIGNKAIWI
                     IVVLFISLIGPILYFTIGRGEE"
     gene            45725..46642
                     /locus_tag="KR250_000942"
     CDS             45725..46642
                     /locus_tag="KR250_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0066907.1"
                     /translation="MNIVSINGLSKGFGNRKIIDNLNFTVPEGSVFGFVGKNGAGKTT
                     TMKMVLGLLKPDSGTIDVCGEKVTYGKTSSNRHVGYLPDVPEFYNYMRPLEYLSLCGE
                     ITGLSKKEIQIRSEELLSLVGLRNEKRRIGGFSRGMKQRLGIAQALLSRPKLLICDEP
                     TSALDPVGRKEILDIMLKIKDSTTVIFSTHILSDVERICDHVAILNKGSIALSGTLSE
                     IKSMHGKDRLLLEFASNDEIQKFKSSDGIKSLLKDSEETNMEIVLHGKDIKAIQKTVI
                     STLAEMNLCPVKMELIELSLENLFLEVVK"
     gene            46639..47397
                     /locus_tag="KR250_000943"
     CDS             46639..47397
                     /locus_tag="KR250_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0066908.1"
                     /translation="MREYIAFTKKEFKENLRNYKLFSLIILFLIFGISSPISAKFMPD
                     LIAHFAPTLKVTAAPTALDSWTQFFSNISGLGMSLTLIIFCNILSNEYSKGTLVIMLT
                     KGLSRSSIVLSKFSVTVIIMTIGFWLSFLFAYGYTMYFWPTANLNHIIFSAFNLWLIG
                     IMYISVLILGCVLFRPAFASVLLVLVATLLLSLISIPKQIAPYTPNFLMSKNIDLISG
                     KVAMPEFIIPIIITVLISIVCLLAAVILFNKKPV"
     gene            47656..48729
                     /locus_tag="KR250_000944"
     CDS             47656..48729
                     /locus_tag="KR250_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HBH0066909.1"
                     /translation="MEKSKVYFCDLYSNSQNKNVPNNVRRLFDEAGFKDLIEKNDQVA
                     IKLHFGEKGNTTYMSPVAVRQVVDKVKDCEGKPFLTDTNTLYTGSRTNSVDHLTTAIE
                     NGFAYAVVNAPVIIADGLYSRNYENVKIDKKHFESVKIGGEIYNSSAMIVMSHFKGHE
                     AAGFGGALKNLAMGCASAAGKQMQHSDVTPVVKEKKCVGCGKCVNSCPTKAISIVDKK
                     AVIDSDVCYGCGECPTVCPTRAVTIQWESDSDVFVEKMAEYAYGAVSNKKDKVGYITF
                     VMNVTPLCDCVPWSGRPIAHDIGILASTDPVAIEQACYDLICKEMGHDVFKHEHPHVN
                     GTRIIDYACEMGMGSKEYELIKL"
     gene            48799..49062
                     /locus_tag="KR250_000945"
     CDS             48799..49062
                     /locus_tag="KR250_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0066910.1"
                     /translation="MKIYYEKENSKKANVNNIPSVFAFNFLRNFLTSKEYEETRKEYF
                     INNLTKSQVNQAMKDLKWLFREYEGLEVITMESLDGVKTRIVL"
     gene            49268..50245
                     /gene="bioB"
                     /locus_tag="KR250_000946"
     CDS             49268..50245
                     /gene="bioB"
                     /locus_tag="KR250_000946"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HBH0066911.1"
                     /translation="MEKYIIKLKNKVLREKEISYEEALNLISLDTNNKNDFDILLKSA
                     NEIREYFMGRKADLCTIMNAKSGKCSEDCKFCAQSSYYKTGVEEYSLLDYNEILNRAK
                     EMESKGVHRFSLVTSGKGMSGKEFNNILNIYEGLRENTNLKLCASLGIIDYEQAKMLK
                     SAGVTTYHHNVETCRDNFHNICTTHTYKDRIKTIKDAKKAGLDVCVGGIIGMNESEEQ
                     RLKMAFEIRELNVKSFPINILNPIKNTPMENYDVLEPMEILKTTAVFRFIIPNVYIRY
                     AGGRLSLKGYDKVGFNGGVNSAIVGDYLTTVGSGIENDKKMIIEQGFEL"
     gene            50473..51486
                     /locus_tag="KR250_000947"
     CDS             50473..51486
                     /locus_tag="KR250_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBH0066912.1"
                     /translation="MSITIKEIGELAGVSKTTVSKVINNKDENISQATREKILKIMKE
                     KNYVPNKLAQSLVTKKTNTIGLLIPDIRNPFFTDVSRGVEDKANEEGYNIILCNTDED
                     AKKEYEGIRTLSERMIDGIIFAASSNTNWKEANYKDIKIPTVLIDKKISMNKETLKGI
                     VKINNFEGAYIATKHLLDIGNKKIIYLSGPLQNEIAVDRLEGYKKALIECNLSYNPAY
                     VFEGKYKIEWGQEFIKNLEKIDFDAIFCANDLIAIGVIRGLKERGLSIPNDISVVGFD
                     DIQTSSLISPSLTTVKQPSYDIGYKASEILINCLRGDKKESFDELIFKPELVIRDSTK
                     ESE"
     gene            51528..52418
                     /gene="rbsK"
                     /locus_tag="KR250_000948"
     CDS             51528..52418
                     /gene="rbsK"
                     /locus_tag="KR250_000948"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HBH0066913.1"
                     /translation="MGNIVVIGSVNMDMVCSVDKRPEKGETVLGNSFFTSPGGKGANQ
                     AISASKLGANVKMISCIGEDGLGEELIRNFRTNKVDYSLVSRNNHKSSGVAVITLCEN
                     DNSIVVVPGTNELVDIELIKKNEEEIKNADIVLLQLEIPLKTINYVVNFCFENRIRVL
                     LNPAPAVKLNEDIIEKVTYLTPNEHEYKIVFDTNEGIEEVLKKYPNKLVITEGKNGAR
                     FYDGEEIKHVSCISVDVQDTTGAGDTFNGALAVAITEGKNLYTAVEYAVVVSGLSVTK
                     LGAQSGMPYKEDVEKYLNNK"
     gene            52466..53428
                     /locus_tag="KR250_000949"
     CDS             52466..53428
                     /locus_tag="KR250_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBH0066914.1"
                     /translation="MRVRKILSLGIAGILAIGMLTGCSMEGPSKSDNKGGSDKKDLTI
                     GVSTITLQHQFFIDIDEGIKEKAKELGVKVIVNDPDQDVAKQTSAIEDFIQQNVDGMI
                     VLGTDNSAIVPAVEGAFEKMPVVTVDAVLNTENITSYVGTVSYDAGKKLGEYTKKYID
                     EKLGGKSEIAIVTDLKSQIQMQRIDGFKDALKGSANVKILNSQPGYDREESLNTVENL
                     IQSNPDVDIIYATAENSVLGAKAALESAKNKDVKIVGFDLTEEASSGITDGTILAMIQ
                     QQPKEMGRLAVEAVVKAIKGEKVEKNIPVPALLYDKENIKDFKN"
     gene            53482..54993
                     /locus_tag="KR250_000950"
     CDS             53482..54993
                     /locus_tag="KR250_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HBH0066915.1"
                     /translation="MSNIILKLSNIAKEFPGVRALDNVNFELFHGEVHALLGENGAGK
                     STMIKILTGAHSKTSGKFIFEGKEIENISPDISKKIGINAIYQELTVFDELTVAQNIF
                     MGKEINGKVLTNDKKMNEEAKKIFDNMGIDINPNSLVKELSIAQKQMVEIARVLSSET
                     KVLIMDEPTSSISKKETEILFRLINDLKESGVSIIYISHRMEELFEICDRITIMRDGK
                     TISTLNTKDVSSEEELVNLMIDRKLDQFFPKRKVEIKEEIMRVENLTKNNVFNDISFN
                     IRKGEILGIGGLVGSKRSEIVEAIFGLRTYDSGKIYLNNEEVKFKTPSDAIENGLGLI
                     TEDRKGTGLFLQMSVKENTTMAGLKKISKFKSIIDRKKEKEILEKYIEALKIKTPHMN
                     QVIQSLSGGNQQKAIIARWLLLQPDILIMDEPTRGIDVNAKAEIYNLMGDLVESGVSI
                     IMISSEIPELISMSDRIMVMREGHISGFLEGEEMVENNVLKLAFGGKINEFNN"
     gene            54977..55975
                     /locus_tag="KR250_000951"
     CDS             54977..55975
                     /locus_tag="KR250_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HBH0066916.1"
                     /translation="MSSITKHKEEKSSPLLTKLTSVFRDQGSAAVGLVIIFIIMSVAS
                     SNFLTLDNLINVGRQISINAILAVGMTFVIITGGIDLSVGAVIALVGTFWATTVVNYN
                     APIWVGMILALAIGTILGVIKGAIISTQKLPPFIVTLAMLTIISGASFVFTGGRPISV
                     NTDAFKMLGRGYIGPIPIPVIIMIIVVIAGHFLLKRTNFGRHVHAVGGNEEAARLCGV
                     KVNKVIVKVYALAGLLTALAGIILSSRLASGSPTVGDGAELDAIAAVVLGGTNMMGGS
                     GSIVGTCIGVGIIGILGNGLNLLSVSSYNQMIIKGLVMLFAIWINNIKLKKSAKSK"
     gene            56159..57421
                     /locus_tag="KR250_000952"
     CDS             56159..57421
                     /locus_tag="KR250_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBH0066917.1"
                     /translation="MKKILLDTLNSKKQEYIDYLKELVSIKTEDVGHGILGGFEKEGQ
                     EYIEKLANYIGFSVDRQEMSEELIKKAKNIYKEGNLGHNYQDRYNLICKYSDDLPGKT
                     IVFNGHVDTMPPGDISKWKYNPYRATEDNGKLYGLGTADMKSGLIASILAVKLIKDSG
                     LNVPGNVKIMSVVDEEGGGNGTINAVMNGIDGDCCIICEPSEQNLIVAHMGFVFFEVE
                     VKGVSLHCGSKWEGVNAIEKAMLLLQDIKELEHNWLMIYKHPLLPSPTINLGVINGGT
                     AGSTVPDKCVFNLCVHFLPNIMSYEQVVNDVTNVIMTRANGDLWLKDNKPNINIYQSG
                     LGFEMDKDSDFVVNAHKILEETLGKKLEIKGSTAGNDARVMKNLAEIPTLILGPGSIE
                     QCHSIDEYVEIKEYLDSILMYASLILNL"
     gene            57441..58184
                     /locus_tag="KR250_000953"
     CDS             57441..58184
                     /locus_tag="KR250_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HBH0066918.1"
                     /translation="MKKVLIVGESWVKNITHIKGFDTFVTTHYEEAVKWLKEAIESGG
                     YETVHMPAHVAADSFPYKLEELNEYDCIILSDIGSNTFLLSNSTFIDCNSNPDRLELI
                     KEYVNNGGALIMVGGYMSFTGIDAKARFGETAIKDVLPITMIDKDDRVEKPAGIIPEV
                     IDSEHPVLKGIPTEWPKFLGYNKTVARDNCPVLATIGGDPFVAVGEFGKGKSAIFSSD
                     CAPHWGPKEFTDWKYYNKLWVNMLDWLTC"
     gene            58357..59742
                     /locus_tag="KR250_000954"
     CDS             58357..59742
                     /locus_tag="KR250_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBH0066919.1"
                     /translation="MDNLNKKFLMEYIDEIQGELRDISDKIWKNPELQYKEYYASNLQ
                     KECLKKHGFSIEGLEGMDTAFVASYGEGRPVIAVLGEYDALDGLSQKVSTTKEPIVDG
                     GAGHGCGHNLLGTGSIGAVIAVKELIKLKKLKGTIKYYGCPAEEDLSGKVLMIKKGFF
                     DGIDCAFSWHPFDINTPIRIPTLANYSVKFRYNGISAHAAQAPYNGRSALDAVELMNI
                     GCNYLREHIFDNCRVHYVTTNGGKMPNIVPDFAEVWYYIRGVKMEHVRDVFGRIVDIA
                     KGAALMTGTIMEYDIISGVYDYIPNTILTDILSQNMKLVGVQNYNEDDYYFADKLAET
                     VSIDKRSSVSSVLSGNKDITKMNLHDEVTDDTFTHNNCISLSLDIGDVSYIIPTAQCS
                     CSVWPIGISAHTWQSCASAGSDMGFKAMLLASKSISCSINDVMLDELVINKAKKELKD
                     TVGSFKYIPII"
     gene            59961..60800
                     /locus_tag="KR250_000955"
     CDS             59961..60800
                     /locus_tag="KR250_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0066920.1"
                     /translation="MFGYVKINKMDLTFREYDYYKAYYCGLCKYLKRNHGEISRFSLN
                     YDITFLIVLLTAVYNPESISTEEVCIVNPFKKKKVITNDITEYAASMNILLTYYKLED
                     NLMDDKRIKDKLAYYIYKNKLKLAYEKYPEKAEYIKQQLNELNKLEKDKNINIDEVSS
                     IFGNIMGEVFVYKKDENERNLRMIGFNIGKYIYLLDAYEDLDEDFKKGRYNPFIEYID
                     KNDELKEKVKKIIVTSLGFLARGIDNLNLNKNVGIIENIIYSGVYLRYINILESRGGK
                     NVQ"
     gene            60790..61233
                     /locus_tag="KR250_000956"
     CDS             60790..61233
                     /locus_tag="KR250_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0066921.1"
                     /translation="MYSNTYNNENFMRARSFIENSEFKKAYDFLKTLTDKCAEWYYLT
                     GFSAMNIGYYEEGEDFLKRAKFMEPENSEYSDALRSYTQYRNDYSNRADNYNRRRRND
                     LDGCCCCCCDDCCCCLGDDCCENCAKLWCLDSCCECFGGDLITCC"
     gene            61244..61735
                     /locus_tag="KR250_000957"
     CDS             61244..61735
                     /locus_tag="KR250_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0066922.1"
                     /translation="MSIKVAYGGVLLALNVILLTLTNIIPVNTLFIMGLASLLVSIVI
                     MEWGFKSGIAFYIGSIVLGFIVMASKSQWIVYSLTFGIYGIVKYLIEKDRSIYIEYFM
                     KLVFANIMILILYFLLRTIVYIPINIFIIGSFEIAFIVYDYVYSSFIGYYNNRLRKML
                     FKK"
     gene            complement(61937..62665)
                     /locus_tag="KR250_000958"
     CDS             complement(61937..62665)
                     /locus_tag="KR250_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HBH0066923.1"
                     /translation="MKNIAAFFDIDGTLYRDSLMVEHFKKLIKYDIIDQKNWYKHARD
                     TFMDWDKRQGNYDDYLLEICDLYVDSLKGLDKTCINFTSDQVIKLKADRVYKYTRSRI
                     EWHLNQGHIVIFISGSPGFLVEKMAKKYNVTDFLGSDYVFENNIFTGTVIPMWDSISK
                     NNAINDFVVKYDLDLSNSYAYGDTNGDINMLKRVGNPIAINPTKELLSQISLDEEISK
                     NVEIIVERKDIIYSLSTDVNIVDI"
     gene            62790..63878
                     /locus_tag="KR250_000959"
     CDS             62790..63878
                     /locus_tag="KR250_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0066924.1"
                     /translation="MNFNFAKATNSRLMGSLGLIINWIDDENNHFCQYFLLDAEGLGL
                     ADYVSLNNPTQEEAYMEEERLMGGFGSDRVELTKDESLFLVSYFGNKNLYYDKLLPGD
                     KCEYIDIIKNYKTDLTIEKLYNKICKRVDEEVEFINYMTMRFIAWDRESLKYFSGSDE
                     IANMHITNINGTLLKNVVSDKGQGRYISEALYEDNDGYYICKIAFCISKCYETGFRIN
                     SLLVTDKEPMYDFEVFDEISKSEFVSVYSVNSPEEFAGVFYRENPFLLKSNMNEGIFF
                     TRFNFNNDHVKENVYIINNDMKAIYYLMGNKFFIGTYNENDCNYINEISQSNYSDYIK
                     FEEKFFFEQNALYDFAESGSLDFDDFLE"
     gene            complement(64315..65163)
                     /locus_tag="KR250_000960"
     CDS             complement(64315..65163)
                     /locus_tag="KR250_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0066925.1"
                     /translation="MSNKKKKDLDTSYMPYLDPANYADYITNEEIPQDELRNDPQISN
                     GYTKIPNNQNINNLYPNNVNHYPKSSNSPNNLPNTNNTSGNMNYNTSATNNMPNNMNN
                     NTGMPNNMSNNMNSNMGMPNNMPNNMNSNMGMPNNMPNNMNSNMGMPNNVPNNMNSNM
                     GMPNNMSNNMNSNMGMPNNMSGNMNNNMYGNTGMPNNMNSNMGYNMNNTMGIPNFSCN
                     QNMPPNVLMMIPGVICHNTMQGMPVVMPSTMPPNIYPTPYGSSNMSVQGIPQATNIEE
                     FDEEEM"
     gene            65362..65727
                     /locus_tag="KR250_000961"
     CDS             65362..65727
                     /locus_tag="KR250_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH0066926.1"
                     /translation="MREEINDCNCNIVHEEIVTEAKSTMPDEEMLYDLAELFKVFGDT
                     TRVKILYALFANEMCVCDIASLLNMTHSAISHQLRVLKQARLVKFRREGKTVYYSLDD
                     SHISQIFDCGLNHIRETYK"
     gene            65750..68137
                     /gene="cadA"
                     /locus_tag="KR250_000962"
     CDS             65750..68137
                     /gene="cadA"
                     /locus_tag="KR250_000962"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HBH0066927.1"
                     /translation="MEANNSIKKEFILGGLNCAHCAEEINNKVSKLQEVKSSNLNFIN
                     KKLTVNIKESFNEDTTIEKIIDIIDSTDPGLDIQISSKENAASKTSIKKELILGGLNC
                     AHCAEEINNKVSKLKEVESSNLNFVNKKLTVNISNNFEEDDVINKIKEIINSTEPGLD
                     IQVGSTDKVKGRTTEKSGAVNDTNKKELIPLIIGALVYIFGIYQTATGYESQFSNIVF
                     IVAYVIVGGDVLLRAIRNISKGRVFDENFLMALATVGALTIGELSEAVGVMLFYKVGE
                     YLQGVAVGKSRKSITSLMQIRPDYANLKVNSEVKVVSPEEVNVGDIIVVKPGEKVPLD
                     GVVVDGVSMLDTSALTGESVLREVEKGDEILSGVINKNALLSIEVTKSFGESTVSKIL
                     DLVENSSIKKSKTENFISKFSRYYTPIVVIAALLIAFVPPLVISGEVFSDWLYRGLIF
                     LVVSCPCALVLSIPLSFFSGIGFASKNGILIKGSNYLEALRSVDTVVFDKTGTLTKGV
                     FNVTKLNPEGISEEELLEYAAIAEVNSNHPIAKSILSYYNKKIDLDTIDSYEEIAAYG
                     IRVKHNGNFILAGNEKLMKKENISYSSAKEVGTVVYIAVDKVYRGYIVISDEVKEDSK
                     NAIRSLKEIGVKEVVMLTGDNEKVAKNIAQELELDTVYSNLLPNEKVDRLEDLYEGRT
                     EKEKIAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVLMTDEPSKISKAIEIA
                     NKTNKIVWQNIIFALGVKIIVMILGAGGVATMWEAIFADVGVALIAVVNAMRAMR"
BASE COUNT        27363 a         7156 c        12428 g        21630 t
ORIGIN      
        1 atattttcaa agagtttatt ataaaattgt aaactatagt gtttataatc tttgtaatta
       61 ttggtaatta aatttaaaaa ataaaatatt taattataca caaaaaagat atttaaaaaa
      121 tttgttattt attgtatact taagttaaaa ctaaataggc aaatctagag aaatctagtg
      181 acgcaaagct atagggacta aggtttatat acataaacta tgtcagccag ttgccaaaaa
      241 gagtcctagg tgtattgtat acctaagaaa agtctataat gactgccttt ttggcagtca
      301 ttttgttatg gaaaaatcac tttatttcag tagaaaatac acataataac atattttacc
      361 aattataaca taaaataaag aaaaattatc tcaaaaagtt actattttac aaaaaatgac
      421 ttctttattt atttaaatta atcgcatttc cattacatta atacaataat gtatcaattt
      481 agtaagaaaa ctaatatttt tttaatgaag tcccatgaaa atataaaaaa attttaatat
      541 gtatttaaat atttcttcga aaagttgctc tttttgtaat atagcaactt catttttttt
      601 attaataaac aaaatagata aaatatattt tgaggagggg tgagtgtgaa tacatatgac
      661 attataaaaa tgggattaga tgcaactaat ctaagaagtc agacaatagc aaataatata
      721 gcaaatataa atactgaagg atataaaaga cattatataa cttttgaaga aacactaaat
      781 gatgctatgc ctaaaataga agaaaaaaca gacaattcta aaggtttaag agtagatggt
      841 aataatgtag acatagaaga agaaaaagta aatcaagcaa tggcaacatt acaatataat
      901 gctttgataa gttttgcaaa tggaaaaata gcaatgacca aatcaataat aagtgggagg
      961 taattagaag atgagtgtgt tcagtggaat gagaatatct gctagtggat taacagcaga
     1021 aagtttgaaa atggatatca tatcatccaa tgtagctaat ataaatgcta caagaggtcc
     1081 aaatggagaa gcatatagga gaaaagttgt ttcatttgaa gaaaactatg ataataaatt
     1141 aggtatgcta ggagtaaaag ttacatctgt tgaagatgat aaatcacctc ttaaagaagt
     1201 atatgaccca tctcatccag atgcaaagcc aaatggatat gtagaatatc caaatgtaaa
     1261 tatattggta gaaatgacag atttaataac agcttcacga gcatatgatt ctaacgtaga
     1321 tacattgaat gctcagaagt ctatgatatc aaaagctttg gaaataggaa agtagggata
     1381 aattatgcca attacacaaa ttaacaattt aatgcaaggt gcaaagttag ataatttgat
     1441 tggagaagca aagccattaa ctcttaataa taaagaagaa aaaaagaatt ttgaagatgt
     1501 aataatagat actatagata aagttaattt tcaacaagta acagcagatg atatgaaaga
     1561 aaaatttata aaaggcgaag atgtttctat gcatgaagtt atgttaatag gtcaagaagc
     1621 acaactatct atgcaatatt taatagaagt tagaaataag attcatgatg catatcaaga
     1681 aatgaataag gtgcaaattt agccttttga acaggagtaa taatcaatga taaccagttt
     1741 taaaaataag attaaggagc aagttgctaa atctaggaca aagataagtt ctttaaaaaa
     1801 gggaactaaa atagcattag gtataggggt tattgctata tgtttagcgc tagtatttac
     1861 agcaatatct gcgagcaaaa ataagtatgc agtcttattt tcagaactag atgctcaaga
     1921 tgctgcagat atatcagcag agctagagtc taaaaagata gatactaagg taaaaggaag
     1981 tacgatttat gtacctagaa cagaagttga taagttaagg ctggagttag cgccaagtct
     2041 tacaggtggt tctaaaggat ttgcactaat ggatgatagt tcatcaatag ggcttactga
     2101 tgaagaattt cagattaaga aacaaagaat gatacaaggt gaaatagaaa aaacaataaa
     2161 gagttttcca caagtggaag acactagagt acatataaca tttggagagt catcagtatt
     2221 tgagaaggag gctgttccag ggagtgcagc agtttatttg atgctaaaac cagggacgac
     2281 tttagaaaaa aaccaaataa agtctgttat ggcacttata tcaggaagcg ttacaaatat
     2341 accagagaaa aatgttcagg ttattgataa caaaatgaat ttattatctg aaggtatttt
     2401 ggaggatgaa aatgttgatg aaaataatca accaataaat agtggcatta gtgttgataa
     2461 aaataaggca tctgaacaag cattgaataa acaactagaa aagtctattt tagaaatgct
     2521 agaaccgata tttggtaagg gaaaggtgaa agctacagta aatgctgatt taagttttga
     2581 tacagctgaa aaaactgaga taaaaattga ccctgataaa gttgcaataa aggagacaag
     2641 gtcgaaaaat tcatctaaat ctgctgcaaa taatgttcaa ggtgtagatg ctaacatgaa
     2701 taaccaagga aataacaatg gtactaatac agaagatagt ttagatgaga gttttgagta
     2761 tgaaacaggt aagattgaaa ctcacactat agttgcgcct ggagaactta atagattgac
     2821 agcttctgtt gcaatagatg gaaaaatagc taaaggtgtt caagctgatg tagaggatat
     2881 tgttaataat gctataggta tggatggagc gaggggagat actcttagcg ttgtttcaat
     2941 ggtatttgac ccagaaggta agaaagaggc taaacaagag ctggaagaga ttaaaaaaga
     3001 aggatttaaa aagaacataa taaaagcagt tattggagta gtagcagtaa tagcagttgg
     3061 agctattgga tacttaatat ataagaaaag aagtgaacga gaagatatgg attatgaaga
     3121 tgaagattat atttctaaat ctgattctgt tgataggaag gataatcctc tattaggaga
     3181 tgatccagaa ctgacattag aggaagctgt taaaatgtat gcagaagaaa agccagacca
     3241 agtaacagag ataataaaga cttggttaaa tgagtaaggg ggatagaaag tgtccacgga
     3301 aattacaaat gttaatgcgt ttgacttaag tgatataaat gaaagtgata agaatgagaa
     3361 ggtagaaggt gagggtttaa aaaaagctgc tgtattatta atgacattag gcccaaatgt
     3421 gtctagtgat ataataaaaa gcttgcctga taaacaagtt caaaaaatag gtttggaaat
     3481 tgcaaatatt tcttcaatag cttcaaaaga aagaagagaa atcttaaaag agtttgtaga
     3541 aataaatagg gcaaaagatt atgttataga aggtgggctt gaatatgcaa gagagttatt
     3601 aagtaatgct ttaggaaatg ttaaagcaaa caaattactt gaaggtatat caataaatac
     3661 aagtacaaag ccttttaaac atattataaa actagatgta aagaagatat tagaaatact
     3721 taggaatgaa agtgcacaaa ctatagccgt tttacttgca catatggaac cagaaaatgg
     3781 agcagaagtt ttatcatact tagattttga tttacaaaat gaagtagcta tgagaataag
     3841 ctcaatgggt tcaatatctc cagaagtaat aaaggtaatt gaaagttcat tagaagataa
     3901 attacattca ataagtggtg atgattttaa tgattatgga ggggtgtcta ccttagttca
     3961 aatattgatt aattctgata gaaaaacaga aaagaatatt ttgggaacta ttgaagaaca
     4021 aaacccggat ttagcagctc aaattaaatc tcatatattt gtctttgaag atattgtcaa
     4081 gttagatgat ataagcatac aaaaagtgtt aaaagaagtg actatgaaag atttagtata
     4141 tgccttaaaa ggtgtagatg atgagatttc agatgctata tatagaaatc aatcttctag
     4201 agcagcagat gcacttaaag aagaaatgga aatggttggt gttataaagg ttacacaagt
     4261 tgaggcttct caacaaaaga tagctaatat cgtaagaaaa cttgaaagag aaggtgccat
     4321 aactattgta aggaattttg gtggtgactt tgttgaataa taagattatt aaaagtaaaa
     4381 aagcattata taaaggagtg ctagatttaa ccataataga taaagttaga gtgggagaat
     4441 caaatgtaaa taaagaaaat gattgtagat ttagggatga ggtaataaaa gaagctatag
     4501 aatctgctga gataaaaaag aatgagatat tagaagaagc aaaaataaat gcacaaaata
     4561 tagaaaagca agcatatgaa aaagggtata gtcaaggtca aaaaaatgga tatgaggatg
     4621 gatataaaga gtcatatgat gaatataccc aaaaagctaa agatgaagca gattttataa
     4681 aaaatcaggc aaatttaatc ttattacaag ctaatgagaa gatgactgag tacataaaag
     4741 aaaaaaataa agagattgta agacttgcta ttaatatggc cagttctgtt ttaaaaaagc
     4801 atttcgaaga tgaagaatct atgagtgatc tgttaaagca aattattcaa gattatgaag
     4861 gaagtagttc atttatagta agatgtaatt ctttctataa agataatata gagaaagagt
     4921 ttgaattatt caaagaggaa acatcactaa aatcaagtgt atttgttatt gcagatgata
     4981 gtatagataa aggcaatgct attatccaaa aggaaaatgg aagaataata gttggtattg
     5041 attgtgctat ggaaaaacta aaagaagaat tgcttggtga ataaagtatg tataaaactg
     5101 atatatctat agattttgac aaaataaata agaaaataga aaatttagaa ctcactatat
     5161 cagaaggtag agtaaagaag ataataggac ttactgtaga agttgaagga ataaaagctt
     5221 ttgttgggga gttatgcgtt atatacaacc aagttaataa accagtgaat tgtgaagtag
     5281 taggttttaa agataaagaa gtcatattaa tggcgttggg agaattaaca ttgatagcac
     5341 ctggttgtaa agttatttct aaggggatac ctttaagtgt tatgtgtagt gataatttac
     5401 taggtaaagt acttgatggt cttggaaatc ctatagacaa ctcagatgct gttttaggag
     5461 atagatacaa cctgaacaat gaacctcctg atccaatgaa gagaaaaaag atacgaaata
     5521 taatggaaac aggtgtaaga gcaatagatg catttactac ttgtggtgaa ggacagagaa
     5581 tcggcatatt tgcaggaagt ggagtaggta agagtacgac tctaggtatg attgctagga
     5641 atgcaaaagc agatgtaaat gtaattgctc ttattggaga aagaggaaga gaagtactag
     5701 attttattga taaagattta ggtgaagagg gaatgaaaaa atctgttgtt gtgtgtgcaa
     5761 catcagataa agcccctcta gttaggttaa aaggtgctct tactgctact gcaatagcag
     5821 aatattttag agaccaaggc aaaaaagtaa tacttatgat ggattcagta actagatttg
     5881 ctatggcaca aagagaagtt ggtgtagcaa taggagagcc accagctcaa aaaggatata
     5941 caccatctgt atttgcaatg ttgccaaaac ttatggagag aacagggaca tcagataaag
     6001 gttctataac tgcattttat acagtattag ttgatggaga tgactttaat gagccaatag
     6061 cagatacaac aagagggata cttgatggtc atatagtttt atctagagac ttggcaaata
     6121 agaatcacta tccttctata gatgtattaa acagtttaag taggcttatg aatgaaattg
     6181 cttcaaaaga agacataaaa atagcgtctt ttgcaagaga catgcttgct gagtatagag
     6241 aagcagaaga tttgataaat ataggtgcat atgcttcagg tacaaataaa aaaatagatg
     6301 aagcaatcta ttatcatgag catattatta attttttaaa acaagggata aatgaaaaga
     6361 gttcttttaa tgaaactata agtagtttga gaagagtttt tgaataaaca attaaaaaaa
     6421 tatttgaata ggttgtgatg gaattggata aaaactttaa gtttagatta cagaaagttt
     6481 tagacttaaa agtgaaagat gaagaagaga taaaaatgga atttgctaag attcaacaaa
     6541 aaaaaataga tatagagact aatttagaaa acttagaaag taattatagc aaatattcta
     6601 taagtaaaaa taacgatagt attcaaaatc aaaaaataac tattaactat ttactagctt
     6661 taaacaattc tataatggat ttatcagaag agttggataa gtcaacaaat gaacttgaaa
     6721 aagctagaaa gcagcttata agcaaacaga tagagagaaa atcattagaa aaattaaaag
     6781 aaaaaaagta tggacagtat tataaagaag aaaatttaaa agaacaaaac actaatgatg
     6841 agtttgcaag tatgagttat ttaaggaata gacaagttct ataagaatag taggaagtga
     6901 ggatattatt gtatgaagat acaagcagat tcttataata ttattaaaga tattggggat
     6961 agaaaaagtt catctaagaa caaaaatata aaaaataata aatttgaaga taatctatat
     7021 aaggcaacag accataaaaa agttaaagat agaagggaag taagtaagca aaatattagc
     7081 tcagataaac cggaaaatag tgataatgag tgtcatttag atgaagataa aagtaatcac
     7141 ttagaagatg aaaatacata tgaaaaagat tgtagtaaga ataatgaagc tttgcagcaa
     7201 ttactgaatc ttttaaagga taattataaa aaagataaag atataaacac tatattgaat
     7261 aatatagaaa aactaagtat atcagatgaa ttaaaacaag aacttaagct taataaaaat
     7321 atattaaata agattgcaaa tcaaaattca gagctaaata aagctaatat aaataactct
     7381 aatgatatag aaaatttaga catagttaaa ttaagagaac ttttggaaga taatgctaat
     7441 aaagatattg aagtaattac taaaactaca gaaaatgata ccaaaaatat agatttgtat
     7501 agttttgata gcaatagaat ggataatctt aactcaaata aacagaaaga tgatagctct
     7561 gatattttgg aaaaactagc aggggtaaat ggaaataaga gtgggaattt tggacaagta
     7621 ataaataaaa atgttgatat gaataaaatt aaaaatgata atgtcaattt aaaacagata
     7681 gaatcaaatg taatggatga ctctataaaa gctattaagc atatgaaaac aaatgatata
     7741 caagaattga caattaaatt aagaccaaaa gaattaggag atatgaatat acaattgtta
     7801 aaagatggtg aaagtatgag agctgttgtg actgttttca ataaagatgt ctttgactct
     7861 ataaataaaa acatagcaga tttaaagcaa catcttgaac ttactaatgt aaatataaaa
     7921 gatgtatcag tgcaaatgca cagcgataat acaaatacat ctgatacctt tgataaagca
     7981 tttgaacaac agaatagaca aaataaccaa gaaaacatga gtaagaagaa tacaaatgat
     8041 aagcataata atgtagtcat agaagatgac attaaagaaa atactattga tgatgataga
     8101 gtagatttac tagcttagta agggggaagt attgcatgta cggaatagca acggctttgg
     8161 caggaactca gggaaactat aggtcaaata acttaagcca acttgaaaga aatattaatg
     8221 atattgccaa taaacaagtt agaagcaaag gaaatcagac agtaacagac aatgggactc
     8281 caattatagg acaaggtgat gatgctgata aagacttgtt tatgaagata cttgtggcac
     8341 agatgggtaa tcaagaccca atgaatccgc aagacccaac agaatatgtg tcacaattag
     8401 cacagtttgc aactttagaa aagatgtcag ctatgaatga gaagctagat gtacttttag
     8461 ccttaagtaa ttccactcta ataaactctg ctttaagtat ggcaacatca ttaattggta
     8521 aagaagtaga agtatctggg gaagagaaag aagatgaaaa cattgtagga gtagtaaagt
     8581 ccacatatat aaaagatggg gaagtacact tagaattaga agtagatgga agtgacaaaa
     8641 ctgtaggtgt taaatatgaa aaattactta aaataggaga ggtagatgaa aattcaggag
     8701 aagaaaatga ttcttcagaa cacgaatctc cagaagaagc ataatattaa atgagaaaag
     8761 aggtaaattc atgataaaag caatgtattc aggtgtaagt ggaatgaaag caaatcaaac
     8821 aaaattagat gtaataggta acaatgtagc caacgctgga actacttctt ttaagaaaag
     8881 ttcagctaga ataacagatt cattttatca aactgtacta tatgcaagtg ctccaacagc
     8941 tgcattagga ggaacaaact taggacaagt aggtgtaggt agtaagatat caagtataaa
     9001 taaagatatg gtacaaggaa atgtacaacc aactaataga ccatctgatt taatgataga
     9061 tggagatgga tatcttccag tagtaagaca aggaacagtt atgtatacga gagatggttc
     9121 ttttaattta gatacaggat ttaaagcagc tgatggtgct ttgaagaata ttaaaggtgg
     9181 aagacttgta aatggtgatg gatatatgtt acaaggtatg atttatgatg gggaatataa
     9241 agctgatact ggagaatttg ttaaaaaggg tgatgctaaa ggaaaagagc ctattgtaat
     9301 accacttcaa agtataagtc ctaaggatgg aacagttcaa aatgtactag agtataatat
     9361 atcaaaggat ggaacagtag aatttttatt gagtgatggt caaagaactc aatttgtcta
     9421 tgatatacct gatggtgcta aggtaggaga tacacctgct ggaatagata aattaaaagg
     9481 tacaatacaa aaagtgcaaa catatgcatt ccaaaatcca gcaggactag atgcagaagg
     9541 aggaaacttg tttaaaccat cagcaaactc aggagaccca caaatagcag gagctacagg
     9601 cacaataata caaggggcaa tagaagcttc caatgtagat atagcagaag aatttacaga
     9661 aatgataata gcaagtaggt ctttccaagc aaacagtaaa ataatatcga catcagacga
     9721 gatacttcaa gaaataataa atcttaagag atagtaggta catattatga taagagtaac
     9781 ggatttaaac ggaagaaaat acatattaaa ttcagacctt atagtgagga ttgattctgt
     9841 accagaaagt aggattctcc ttacaacagg agaaaaaaac cttgtaaaag agtcggtaga
     9901 tgagatagta gataaagtaa taaagtataa aagaaaaata aatgtaggtt atattaggag
     9961 cgaaaaataa tgaaaaaaac agattcactg acatttattg gactaatagt atctacagta
    10021 ttagttttag ttggtgccgc aaaaggaagt agttcagggt taaagaattt ttttgatgtt
    10081 tcatcaatat taatcacagt gcttggttca tttggagctc taatgattac atttactata
    10141 gatgatataa aacttataaa aaatgcatta caatactcat ttaagacaat gagtgtatca
    10201 aagttagatt tactagaaca atttaaaaca ctttctaaaa aagctagaaa agaaggtctt
    10261 ttatcaatag agagtgatgt aatgggaata gaagatccat ttatgaagaa agggttagag
    10321 ctgtgtattg atggattgga aatcaatgag ataaggtcca tcttagaact agagatagat
    10381 tctgttgaag atacaaataa cagatactct aaactattta aaacttgggg aacctttgca
    10441 cctgcttttg ggatgttggg aacactaata ggtttaatac aaatgttagc tgaccttaca
    10501 tctccagatt taatagcatc tggcatggga aaggcattaa ttacaacgtt ttatggttct
    10561 ttacttgcca atatcgcttt gaacccaatt gcatataata tagatgagaa gactgaaaaa
    10621 gaaatttatg taaaggaaat gatgttggaa ggtataataa gcatacaatc tggggagagc
    10681 agtatagtag ttgaggaacg tttagccaca tacctttcaa acaatgagaa gttagaaatt
    10741 atgaaatcca ataaaaatac agaaagagca atgagcaatg gcgcgtaaaa aaaataagaa
    10801 aaaggatgag ataaatccag atgcatggct ggctacttat gcagatacta taacactgat
    10861 tcttacattt ttcgtattat tgtatgcaac atcagttgta gatatggaaa aatttaaaaa
    10921 catagcagat gcgttaaaag gtcagttttc tgggacaagt atatttagta cgggttcaag
    10981 tacaagtgag aaatctccaa tagacagtgt tttagtggat aaaaatgata aatcaaattt
    11041 agaacaggat ttaaaagata aagttaataa ttctaactta caaaatagtg taacagtcaa
    11101 agatgatagt agagggattt tactagagtt agatgattca attttatttg actctgggat
    11161 ttctgagtta aaagtagata gtaaagctac tcttaataaa gtatatgaca tgataaagat
    11221 gatgaaaaat aagatagtta tagaaggcca tactgataat gtacctgtaa aatcaagtac
    11281 acatgaatcc aattgggatt tatcttctag tagagcagta agtgttgtaa gatactttgt
    11341 agaagaaaag ggcatggacc ctagactatt ttcggcaaca ggatgtggag agtatcaacc
    11401 tttggtttca aatgaaacag caaagggaag ggctgagaat agaagagtta atatacttat
    11461 aactattgac taggaggaag aggtttgttc aaaaataaaa aaattattat aattattttg
    11521 atactgatta ttttctcagc gggagcaggt ttttttatat taggagaagg tggaaataaa
    11581 caagttgaca agattgcaga tttgtttatt ccaacaggaa aaggtaaaga tgaagtaaag
    11641 accgaaaatc ttgacttaga agagatggta ttaaagctag ctgatgaagg tgtaagatat
    11701 ttaaaagtat caataactgt atcttatgat tcttcatata aagatataga aaaaaatacg
    11761 cctttaatta gggataatat tataaattgt tttatggcaa aaagggcaga tgattttact
    11821 gctgataatt taaagaacat aaaagaagaa ataaaaaata cattaaatat acaactaggt
    11881 gaaaatgtaa ttcaaaatat atatttcaca aatatagtag tgcaataaaa ggatggtaag
    11941 acatgatact ttatttacta aaaatattaa tatgtatccc aataatttta atattgatag
    12001 tcttatcttt aaaaattagc caaggaactt tattaaaaaa atataatgat aaatatgtaa
    12061 aaatactaga tattgtaaat ataagtaaaa acaatagcat aataatttta aaaataggtg
    12121 aagaaggatg cattatggca tcttcttctt ctagcatgag taaaataaaa gatttgacca
    12181 aagaagaaat attaaaaata gaaaacgaca ttaaagaaag aaattttaaa attcaagatt
    12241 ttaaaatgga aagaattaaa aagaaatatg atttcagtag atatattgga aaatttacaa
    12301 aaaaggaaga taagtaaatg gaactattat caaaattacc tttggctgat gtaccatata
    12361 caagttcaat gcaattgttt ttatttctaa ctgcattaat gtttttacca tttgtaatgt
    12421 tcatgatgac aagctttgtc agaatagtta taagtttatc atttttgaaa tcagcattag
    12481 gagcacagca agcaattcca agtcaaattt tggtaggact tgcaatagta ttgacaatat
    12541 ttataatgag accagtatta aatgagataa acgaaaaggc attacaacca tatatgaaag
    12601 aagaagttac tatggaagaa gctatgaagg aagcagaagg acctattaaa gagtttttat
    12661 taacacaaac cagacagaca gatttagatt tatttgtaga acaggctggt ttaaaagaaa
    12721 agaaattaac tcgtgaaaat ataccactat ctgttgtagt tccagcattt gctataagcg
    12781 aactaaaaac tgcttttcaa ataggttttt taatatatat accatttttg ataatagacc
    12841 ttgttgtggc aagtgttttg atgtctatgg gtatgtttat gttgcctcca gtcatgatat
    12901 cattaccatt taagttatta ttatttgtaa tggtggatgg atggaacttg atagtaaaaa
    12961 cattgatatt gggatttgga taggagaaaa atagtatgag tgaatttatg ataatgagtg
    13021 ttcttaaaaa tggcatgttt acaggagcta tgattgcagc tccagtactc attgtttctc
    13081 ttgtagttgg gcttttaata agtgtgtttc aagctacaac tcaaatccag gagcaaactt
    13141 tgacttttgt accaaaacta atagctattc ttttagtagt agctattggt ggaagctgga
    13201 tgttacatac tcttattagt tttacttctg agatattcaa tatgatatct cttataacta
    13261 aataagtagg atacatatga tttttgtatt tattagaata gtagcattct ttggaacact
    13321 aagaatacta tttccagctg gtacacctgc tttatttaag tcactctttg caatcattat
    13381 atcaatattg atttcaagta ctatgcaaat tgaatatgca acaaatatag acaacattgt
    13441 attgtttacc ttatatggtg taaatgagac cataactggg attatgcttg gatatataac
    13501 caacctatgt ttttatagta tcagaatggc gggttctatg atggaccaac agatgggatt
    13561 atcaatgatt aatatgtttg acccaaattc aatgactcag acaactttaa ttgataatct
    13621 tatgaactgg acagcactta tgatattttt tagcatggat ggtcatcatg ttttaattag
    13681 aggtataagg tatagttttg agttaattcc aataggaaaa ccatttgtag ataataacat
    13741 agattatata ataaacatat ttgtgcagtg ctttttaact ggattcaaaa tagcaatacc
    13801 tattgtactt tgtctgctta tggcagattt tattttaggg cttatatcaa gaagtgtacc
    13861 tcaattaaat gtaatgatag ttggtatgcc attaaaaata ttggttggta ttgcattatt
    13921 tatcatatca atacctttaa ttgtcaatca aataagtcat ttattaagtc aaataccaaa
    13981 gatgtatgag ggaacttttg cactagctcc tatgtttttt atgggttcag cagataaaac
    14041 agaagaagct actccaaaga aaaagggaga acaaagaaag aaaggtaata tagcaaagag
    14101 tagagaatta cccgttgcaa tgactctact tgcattcacc ttattagtac ctacattatt
    14161 ttcatatgta gtagatacac ttaaatcttc gcttaattat tttttgagtc tagattttta
    14221 tatgaacata aattattcaa acctagagaa attagttata gcaggattaa tggatttttt
    14281 taagatattt ttaccaatag ctattccctt tttagtttta ggcataatag caaacctatt
    14341 tcaggtggga atactgttta ctggtgaaac tttaaaacca aatttatcaa aattaaatcc
    14401 aattagtgga ttcaaaaaca tgttttctat gagaagtttg tcaaccttaa taaaggatac
    14461 agcgattata tctattttgg cttacatagg atatacattc ttccaagata attatttaga
    14521 tatattaaag ctaggtaaca tatacctacc aactttaatg tacacagtta aagacttagt
    14581 atattcaata ttgagtaaga tttgtgtagc tatgatagct atagcagttg cagattatgt
    14641 ttatcaaaga tacagtcata aaaagcaact gagaatgaca aaacaagaag ttaaagatga
    14701 atataaaaac tcagaaggag acccagaagt aaaagctaag ataaaacaaa aacaaagaca
    14761 aatttcgtct caaaggacaa tgcaagcagt accaagtgct actgtaatag taaccaatcc
    14821 aacccactta tctatagcag tgagatatga aaaagggaaa gaccaagctc cagtagtggt
    14881 ggcaaaaggt gcagattatc tagcttttaa aataagggaa attgcaaagg gaaataacat
    14941 ccctataata gaaaacaaac ctattgctag gttgttatat aagcaagtag aaatagacca
    15001 agaaattcca gaggacatgt atcaagcttt tgcagagata ttagtggcag tatataaaat
    15061 caaaaataga tacaaagtcc ccaaaaggta ggataagtaa tgaataagtt taatttaaag
    15121 gaaaatatgg atgtaatagt ggcatttggt gttgtaggga ttgttcttat gatgatagtg
    15181 ccattaccta catttatatt agatatttta ctggcaatta acataagttt atctgtgcta
    15241 attttactaa tgacattatt tacaacaaat gtacttgatt tatctatatt tccaacagtg
    15301 ttacttgtga caacactatt tagattggga ttaaatcttt catcaactag attgatttta
    15361 acacagggat atgcaggtga agttatagag gcatttggaa gttttgttgt aggtggaaat
    15421 tacgtagtag gaataattat attcttaatt atattaattg ttcaatttgt tgttataacc
    15481 aatggttctg gaagagtatc tgaagtaaca gctagattta cgttagatgc catgccaggt
    15541 aagcagatga gtatagatgc agatttaaat gcaggagtta tagatgacaa aactgctaga
    15601 aaaagaagaa aagagcttca acaagaagca gatttttatg gttctatgga tggagcaaat
    15661 aagtttgtaa agggagatgc catagcaggt cttatagtaa cagcaataaa cataataggt
    15721 ggaattgtaa taggagctgt aatgctgaat atgactctta tgggtgcagc acagacatat
    15781 gtaagactta ctatagggga tggtcttgta agtcagatac ctgctctatt aatatctaca
    15841 tcagcaggta taatcgtaac aagagcttct actgatgaaa actttggtga cttagtaggt
    15901 aaacagttaa cggcaatacc aaaagcagta atgatgactg gttgtgttct aatcgtactt
    15961 ggtatgatgc ctggattacc taaaccagca tttttttcac taggtatagg agctatagca
    16021 gcaggttact ttttaggaag agaaaagaaa gatactttag aagacttgga gtttgaaaaa
    16081 cctgatatgg taaacccaga taaaaatgaa gtagaagatg taacaaattt aataaatgta
    16141 gaatcaatag aagtagaaat tggatatggt ttaatatcct tagctgatga atcagcagga
    16201 ggagatttac ttcagagaat agcatcaata agaagacaat gtgcaataga tatgggtata
    16261 gtcgtacaac ccataagaat aagagataac ttacagttaa atccaaacca atacaatata
    16321 aaaataaaag gaaatgtagt tggtgggtat gagataatgc ctaatatggt tttatgtatg
    16381 gaccctatga atcaaggatt tagtatattt ggaataaaga caattgaacc tacttttgga
    16441 atagaggcac tttggataga aagtgacaag gtagaagatg cagagttaaa gggttataca
    16501 gttgtagatt catccacaat acttataact cacttactag atataataaa atcaaatgca
    16561 cacgaattac tgggaagaca agaagttaag actattatag atgcagcaag agaaaattat
    16621 agtgctgtag tagatgaatt gataccagat ttgatgacac ttggtgaaat tcaaaaagta
    16681 cttaaaaatc tgttaagaga aaaagtaaat ataaaggata gagttacaat acttgaaacg
    16741 cttgcagacc agtctagaaa tacaaaagat atagagcttc ttactgaata tgtaagaata
    16801 gccttggcta gaagtatttg taataattta gttgatgatg ataagacaat tgtagtggct
    16861 acattgtctc cagaaactga agagttggta tctaataatt tacagaggtc tgtaaatgga
    16921 acttatccag cagttgaccc agaaaataca aataaaatat ttgaaagtat acaagaagta
    16981 atgagcaatg tatatttcaa tcataatata ccagtaatag tagtatcacc aaagataaga
    17041 gctccattta gaaaattagt tgaaattgta tatcctaatt tgactgtatt atcattaaat
    17101 gaaataccta acgatattaa aatcaaagca gaaggtgtgg tgagcatata tgatgacgaa
    17161 aaagtataca gctaacacaa ttcaggatgc tatgaatcta gccaaaatgg aattgggtga
    17221 caatattaca ttaatagata aaaaagaggt aagaaagtct ggaatacaag gtatcttttc
    17281 aaaaaaagat attgaactaa ctattggatg ggaaaagaga gaaaatgtag aacaaaaaga
    17341 tttaaaacga gagatagaac aattaaagtc aattattaac aatatggggt ttgataataa
    17401 gaatgataat gatatagata aaatatgtaa aaatctacta agtttagaat tgaatgaaga
    17461 aattgtagaa tctataagaa ttgatttgca agaaatgaag ttcaatggaa tcgatacaag
    17521 taaaaattta gtagaaattt taaagaaaaa gattaaaata gaaaatcaag taataaatgg
    17581 taaaattgct ctagtcggac caccaggagt aggtaaaacg actacaatag caaagttagc
    17641 cgcaaaatta gtttttgaag aaaataaaaa agtaggagta ataactatag atacatacag
    17701 gattggtgct gtagaacagt tgaagatata tactgatata atgaatattc cctttaaagg
    17761 ggtaataagt cctgatgaaa tggaattggc acttgacgag atgaaagatt gtgatgttgt
    17821 attaatagat acaacaggta gaggctacaa aaactctatg caaatactag aaatcaaaaa
    17881 tcttatagat aaagctgaaa ctgataatat acatttagtt ttgaattgta caacaagaga
    17941 aagtgataca aaagcaatta tagattcata tagaaatgtg aattttaaaa gcttaataat
    18001 tacaaagcta gatgaaacaa taacatatgg ctcaatattt aacataatga attatgcgca
    18061 aaaaccaata tcatatatta caacaggaca aaatgttcct gatgatatta taaaacctaa
    18121 tgaagataag attataaggt tactattagg agttgaaagt atatgataga ccaagctcaa
    18181 attttgagaa aaatagcaat ggaaaaaaga ggacttgatg agtttatagt agagaatgag
    18241 aatgtaccta agataattac aatagcttct ggtaaaggcg gtgttggaaa gagcaatctc
    18301 gctacaaatt tgtctatatg cttgaccaaa cttaataaaa aagttcttat attagatgca
    18361 gacataggaa tgtctaatat agatataata atgggggtaa atgtaaaagg gactataatt
    18421 gatgtaatta atggcgaaaa aaatatagaa gatataattt cacagactaa gtatggtgta
    18481 aatataatat ctggaggttc tgcccttaat catatagaag actttacaga agctcaaaga
    18541 aataagttta tacatagcat agaacaaatt cacgatgtgg attttataat aattgataca
    18601 ggagctggta tgagcaaaag cctattgtca tttatatact gtagtactga atttttctta
    18661 ataacaactc cagaaccgac atcattgact gacgcatata gtttattaaa agctataagt
    18721 aattttggca taaagagaag tgcaaatata ataatcaata gggtaattga tttagaagaa
    18781 gcaaaatcta catacaaaag aataaaaatg gttgtggaca aatttctaaa ccttaatcta
    18841 gaattgtatg gatatataat agatgacaaa aaagtaagtt tatgtgtaaa aaaacagata
    18901 ccattcattt tagaatatcc tacaagtatt gcatcaaaat gtattatagc catagcaaag
    18961 agaatggtaa gtcagtcaag agaagatatt gttgataata aaagttttct aagaaagatg
    19021 tttagtatat ttaaaagcta aggaggcgta gttaatgaat agagaagaat taataaaaga
    19081 gaatatgcct cttgtaaaga gtatagcaag aaaatttttt ataccaggga aaggttttga
    19141 atatcaagac ttagtaaata gtggagtcat tggattgatt gatgcaataa ataaatttga
    19201 tgctgaaaaa ggggcaaagt tttctagtta ctcttatata aaaataaaat ctgctatatt
    19261 agatgaaatt agaaatcaaa gtccaatttc taaacataat ctaactaaag taaataagta
    19321 taatagagtg gtagaaaagt tgcaatctaa attattaaga gagccaactt catatgaaat
    19381 tgcaaaagaa ttaaaggttt cagaaaaaga acttcatgat atcgaaagta atatagatat
    19441 gttaaatatt gtatcattaa actatgtaat ctttgaagat acaaatgaaa ctgtacaaga
    19501 tgtaataagt gatagagaag aggaagctcc agaaaatata atagaagaag aagaaaagtt
    19561 agagattcta tccaaagcaa ttagtaattt aaatgaaaga gaaaaattaa tactttcact
    19621 gtactattat gaagatttaa atctaaagga gatagggaaa gtgctaggtg tttcagaatc
    19681 aagagtttca caactccaca gaaaatcaat aagaaactta agaaacaaga taaaagaact
    19741 taaatattct atatagatgg tgattgataa gatgatacaa attatattaa tagtagtgtc
    19801 tattttaata atagtaacta tagttataaa tttacataag gaatcaaaaa ataaagataa
    19861 cagatacttt gataaaatat ttgagcaaga atatgaagca aatgatagat taataattgc
    19921 agaaaaaaga agattctttt tagacaaagt acttgcagaa aaaagaatta atagtcatat
    19981 aaaagtttat gaaaattata acctaataaa ggctaatgaa gaaagaaagg aaaacatatt
    20041 tatagaatca aaaaaggaag taagacaaga aaatgagtta tctcttaaag tgaaacatct
    20101 tataagttca ggttatgatg atgtggaaat atgtaagatg ctagatattg gaaaggggga
    20161 actgttatta ataaggagtt gttacaaaat ataaggatat tattatgtga taaaaaagta
    20221 ttcttaggaa taggtattgg tatattaata tcaactttat gtataatgct ttttagtaat
    20281 aatgatatga gtaaggcaga gatagaaaaa aaggccagag cgttaggcat gaaatataca
    20341 agtgagatga aagttttaga atagatgata ttgtttagac atttacaaaa aaggaggtca
    20401 taatgtgata agaggattat atacagcagt ttctgctatg ataactaatc aacaaaagca
    20461 aaatgttgtt gtcaataacc tagctaatat ggatacaaat ggatataaaa gtaaacaact
    20521 tttgactaaa agttttgatg aattaaagtt ggagggttac aataactacg caaatggaac
    20581 taaacaaaag cagattatag gcgatataag tccaggtatt tctatggatg aaactatcac
    20641 aaattttaat caaggaccta taaaaactac tgataataaa atggatgtag ctattcaagg
    20701 taagggattt tttctagtta gtgatgcagc tggaaatcaa ttttacacta gaaatgggaa
    20761 cttcagaaca gacaatcaag gtgaccttat aacaagtgaa gggtattatg tacttgggac
    20821 gaatactgct actggtgctg taggtcatat aaatgtagga aatcaaaaat ttgaagtggc
    20881 aaaagataat acaatatcac tagacggaaa tgctatgtat aaatttaata ttgtagattt
    20941 taataactac aataacttaa aaaaagaagg agataactta tattctggag gaggagctat
    21001 gaaagtaaat aatgcactta caatacaaag tgcgatagag tcctctaatg tagatatggt
    21061 atctgaaatt aataatatga tgacaatatc aagagaatat gaggcaaatc aaaagataat
    21121 tcaggctatg gattctaaat tagcaaaaat agcaagtgag attggttcag taagataggt
    21181 aataggaggt gaatttttat gtacacaatg ttttatacta gtaaaacagc tttatcagca
    21241 aatcaaagta aactagatat aatatctaac aatatagcaa attctgattc aacaggatat
    21301 aaaaaaataa atatggaata ttctgattta gtggatgaag tactaaatag accatcatat
    21361 cctacaaatg gcaatgatat atcgactgga acaggagcta aggcaacagc tcccatgaga
    21421 aattatgaac aaggtgcatt ggctcctaca gatagtaaga gtaatttggc aatagatgga
    21481 gaaggattct ttagagtgat aagaaaagat ggtacatatg catatacaag aaatggtgga
    21541 tttaatgtag atgctttagg taagattgta gatgataatg gaaatatatt agatgtccaa
    21601 tttgatgcag gatataacta taataataca aatatagata gtacaaacct gacagtttca
    21661 agaaatggag agctattcgt agataataaa aaaataggaa aaatcaactt atatcaacct
    21721 ataggaactc aaaattttat atctgaagga gatagtttat tcgtcgcaac tgatgcagca
    21781 cagattaaac aagttgagaa agtcgatata gttcaaggct atagagagag gtcaaatgtt
    21841 tctttacaag aagaatttgt tgacttaata gccactcaaa gagcatttca aatgaactca
    21901 aagggaataa agacagcaga tgaaatgtgg caaatagcga ataatttaag agctaaataa
    21961 ttatgtaagg tgaggaatat tcatgaatga tatgaaagag tacgattttg gcaaacctca
    22021 gagttattca agagaaaatt taacttgttt aaatttttta ttagctgaat tttgtaaaaa
    22081 atataaaaac tatataattt atgaacttaa gtgtaattct agtatggttg ttgatagtat
    22141 agaccaagta aattatcaga cttttttaga tagagtaact tcttcatgcg tagtagtaca
    22201 aaatgcaatg gaacctgtta tgaaaaactt ttcttttaga atagatagaa gtgtagctga
    22261 tatgtggatt gatataactt ctggaggaac tggagttgta aaggataatg atagagaact
    22321 tacagaactt gataaaaaag tgcttttaca ccttatggat gatttagtaa gaaatatgca
    22381 cttatttgag ggttttgaaa atatacaagt attggataca catacacata ttaatttgcc
    22441 acaattatgt tctccaactg caccagtatg cgttgtagat ttaaaagtgc taaatgataa
    22501 tgagtatatt ggaagtgttt ctctttgctt tccatacaat ggtttagagg ctctgttaga
    22561 aagtatatct gttatagaat tttttgataa tgatgtagga gatgattcag aggaatttac
    22621 ccaaaaaatc tataatagtg tatctaatat agaattatca gttattgcag agataggaaa
    22681 agtatcaatt aatgttgagg atttactcaa actagaagtt ggagatgtaa tagtaacaaa
    22741 taaaaaaata aatgattata tagatatatt tgtagaaaat tcaaaatcat acacagcaac
    22801 accaggattt atatcaagta aaaaaggtgt aaaaataaat gatgctgttg gaaaagaggt
    22861 gtagttatgg acaatagtaa tcttatttca gatgaagaag caaagatgtt attaaatgat
    22921 aaagttgaaa gtgaagttga tggagatgat gagttagcct tagatgtctc tgaagttaat
    22981 cgaaataata tacaaaggat acttgacctt aagttagaat tatctgtagt tataggaaga
    23041 actaagatga ctctaagaga tattttaaat ttacataaag gttctcttat tgaactggat
    23101 actttggcag accaagatgt ggaaatacta atagataata aggttttggc ttatggaaaa
    23161 gtagtggtgg tagatttaaa ttttggtgta aagataacaa gtatagtaag tgaagaagac
    23221 atggtaaaat ctcttgttta gagataacat caattaatac ttaaggcatg aggtggtact
    23281 tattttaaac ttaaatgata taaaaaaaga tatactaaaa ctcaataaaa aagactcatg
    23341 ttctctatat aatgaatcta ttaataatat aaataataat tgtatatcaa ctggattaaa
    23401 atttttaaaa caagcaagag atttaaaccc agatgatgtt gatatattaa atctcacagg
    23461 tcttgtaaac ttactaaaat gtaattttga tgatgctgta gaaagtttct ataagagttc
    23521 actttgtgaa aaaactgctc tgtgtgaaaa gtatataaac atgcttacat ctgaggagtt
    23581 tttaattttc tttgaaaagt acaatcaact tataaggtta ataaataaag gaatgtataa
    23641 agaagctata caagggttta aacttataac tgaccaatac tgtgatttaa tagaacctta
    23701 tgagttgctt gccttactgt atgacaagga agaagaatat ataaaatttg atgaatgttt
    23761 agaagtattg aagattattg ataaggaaaa ttatttatta aatcaagaaa ataacacatt
    23821 atatatgtat aatagtgcta atatacaaac taaaagtaat ataaaagtag atgattataa
    23881 gaatgagttc ttacataaaa gtagtagtaa ccataaaatt aatgattata atgatgtaac
    23941 taaagaaaag agttcaagca aaaaccagaa aagttcagat tctaataaga aaaaaagtat
    24001 taaaaaaaat aagtacctat atatagccat aatattagga atattattag taggacaaag
    24061 tatatatagt tcttataaga tgaataattt aactgataaa atatctaaaa ataatgaaac
    24121 taaaaatgaa gagtcaatag attctaactt gactaagaaa gaaaataata tagaagaaaa
    24181 tgaagatatg agtaaggctt ctaatataaa taagagtaat aaaaacatta ataaagagga
    24241 tgatgtaaaa tctaaaaaaa atactgtaga aaatgaagat gataaagatt ctgacttatt
    24301 tacagaggat gaattgatgt ctaaggcgaa tactttaaaa tcagaaaaga aaactaaatc
    24361 ttctatacaa ctatataaga aagttgcaga tataggtgaa aataaaggga atacatctga
    24421 agcaacttat caagttgcta ttctcagtga aaagttaaaa gactataaaa ctgctgaaga
    24481 atattataaa atgtatgtag aaaattactc agaaaaagat gcttattttg atgaatctta
    24541 ttataattta agtatgatgt actataacaa tggtgattta aaaaattcta aattgacatt
    24601 aaagaagttg gttaataagg taccaaatag tatgtataat aattcaaaag taaaagaaat
    24661 tttaaaagaa gaatagttaa attagataag aaaaataaat tattttattt taaaataata
    24721 tgattataat atattgacat accattttaa aaaatttaaa atattaatta taaaatgagg
    24781 agggataaaa aatggaattt gaaaaaggga gtatttcaat tcatacagaa aatatttttc
    24841 ctataattaa aaaatggtta tactctgata aagacatatt tataagagag cttataagta
    24901 atggatgtga tgcagtaagt aaacataaaa gacttgtatc actaggagaa atatcagaaa
    24961 ataagtcatc agattataaa attacagtat ctgtaaataa aggagaaggt acacttaaat
    25021 ttattgacaa tggaattggt atgactgaag aagagataaa aaaatacata aatcaagttg
    25081 ctttttcagg tgctgaagat ttctttaata aatataagga taaaatggaa gagtcaaatg
    25141 atataatagg tcattttgga ttaggatttt attctgcatt tatggtgtct aaaaaggtac
    25201 aaatagatac actttcatat acagaaggag cgactcctgt aagatggata tctgaaggtg
    25261 gtacagaata cgaaatatct gaatcagatg ctagaaatga tagaggaact actataacac
    25321 tatttattga tgatgatagt aaagaatttt tagatgaatt tactgtaaga ggaataataa
    25381 ataaatactg ttctttctta ccagtagaaa tatatttaga agatgttgaa agattagaaa
    25441 gagaagctaa agaagcagaa gaaaaagcta aaaaacaaaa ggaagatgga aaagaagaga
    25501 ttgtagatgc aaaagtaatt gagcctttaa acgacactaa tcctctatgg ttaaaatctc
    25561 caaaagactg tactgatgag gagtataaag agttttatag aaaggtattc aatgtatttg
    25621 atgaaccttt attttggata catttaaatg tagattatcc tttcaattta aagggtatac
    25681 tttacttccc taaattaaaa aatgagtttg aactgactga aggaaaagta aaattataca
    25741 acaatcaagt gtttgtggct gacaatataa aagaagttat accagaattt ttacttttat
    25801 taaaaggagt tatagactgc ccagacttac cattaaatgt atcaagaagt ttcttacaaa
    25861 atgacaggga tgtaagcaaa atttctaaac atataattaa aaaagtagct gataagttga
    25921 aatctctttg taaaaatgaa agagaagaat acaataagtt ttgggatgat atacaaatat
    25981 ttattaaata tggttgctta aaagatgaaa gtttctatga gaaggtaaaa gaatgtatat
    26041 tattcaaaac tattgatgat gagtatataa cattacaaga ttatttagaa aaatgtaagg
    26101 acaaacatga aaataaggta ttttatgtaa gtgataagga acagcaatct caatacatta
    26161 aactatttaa agaatacgac ttaagtgctg ttgttttaaa ctcatcaata gatactcatt
    26221 ttatttcatt tatggaatat aaagagaatg gagttaagtt taatagaata gatgcagacc
    26281 tttctgatgt attaaaagat aaaaatgaaa ataaagatag tgaagaaaac aaagaagaga
    26341 tagctaagat agaaggtctg tttaaagaag ctgtcggaga aagagtgaag aattattctg
    26401 tagaaggatt gaaaaatgaa gacactccag ctatggtatt agtatcagaa caatctatta
    26461 gaatggcaga aatgcagtca agatttgctg gaatggattt aggaatgaat tttgaggaag
    26521 aaaaaactct tgtaataaat gagaacagtc caataatcaa aaaattggtt tctcttaaag
    26581 atgatgagga aaagaaagat aagattacgc ttatttgtaa ccagatagca gatttagctt
    26641 tactttctaa taaagaatta aagccagatg agttagattc atttgtacaa agaagtaata
    26701 agcttatgag tcttttgata gaattataag attgaatttg aatacattga agcaaattta
    26761 taaaaagtat tcaatatcag tgataagaaa gaagtgtctc aaaatgaact tttcagttca
    26821 tgaggcgctt tttttatgtg aaattaaatt tattttaatc atttcaacaa tgaaaaatat
    26881 atttatctct attttgagac agaccattct ttattttttt atctttggtt aattttaaat
    26941 atttaagtag gttctaataa aaaactctat ttagttaaat atagttaact tgatatataa
    27001 taagataata catataatta agtaaaagat tgttttttag tttatgttta aatacataat
    27061 tacactttgt taaaaacata aaattattac taaagtagaa ataggaggtt ttagaatgaa
    27121 ttttaattat aatttgccag taaatatctt atttgggaga ggcagaatta atgatgtggg
    27181 aaaagaagta tctaaatatg gcaaaaaagt attgatagta acaggtaaaa atagtacaaa
    27241 gaaaactgga ttacttgata aaacaataga tttactaaaa gactctaaaa tagaatatga
    27301 agtttttgat agagtagaac aaaatcctct tataactact atatattcag gagtagaaat
    27361 tataaaatca actggatgtg attgtgtttt aggtcttggt ggtggaagca ttatggatgc
    27421 tgccaagagt atagcttttt cttataaaaa cccaggagat ttaaatgatt atatatttgg
    27481 aattagacaa ggtgaagaag ctttacctat aatcttagtt ccaacaacat caggtacagg
    27541 tagtgaagga aattgttttg cagtacttac aaattcagaa actaaagata aaaaatctat
    27601 aaagaagaat agtatgtatg caaaagcatc aataattgac ccagaactta tggttacaat
    27661 gccaaaacat ataattgcat cagttggatt tgatgcactt gcccataata tggaagcata
    27721 tctttctaat ggaagaaacc ctcttgcaga tgtacaagct atttatggga ttgaacttat
    27781 atcagaaaac ctaataaagg tatataatga tgttaatgat ttagaagcat gggaaaaaat
    27841 aacacttgca agtactattg gaggaatgac gattggtaca gcgggtacag ccttgccaca
    27901 tggaatggag catcctgtaa gtggtcttag agatgtaatg catggaaaag gacttgcagc
    27961 tttgactcct actattatag aaagttcatg ggatagtgat attgaaagat ttggtaatat
    28021 ttcaaaggta ttaggaggaa cttgtgcaga agattgttca gatgcaatta gaaatttcct
    28081 taaaaaaata gatttagata taaaactaag tgatttaggt attgaaaaat cagatataga
    28141 ttggttaact gataattgta tgaaaatatc agttgcaaat attaagagac atccaaagta
    28201 ttttaataag gaacaaataa aggaaatgta ccataagtct ttataatttg actttacata
    28261 tttagtttta tataaaaatt ataaattaat aataaaaaat tataagtaat taatagtaaa
    28321 aaaattgtgt atagaagtta tatatataat aaatttgtct taagctttta ttttactttc
    28381 taataaataa aggagtttaa aaaagggaag attaattaag taatatttta taaaattctt
    28441 cgatttaaaa actttaagta gaggtgaaat aaatgtttaa tcccaaaaga ataatattcg
    28501 aaaaaggaac tttagacact gatgtgggta aaaaaatata caataaattt agagataata
    28561 aagatgtaga gataattaat atatcatcaa ataggattaa agaacatatt ccaggtgaaa
    28621 atctatatga acaatataga agtgggaagc agactatggt agttgggatt agaaaaagtc
    28681 tgaagtttca aacatgtaaa ccttctgcta attatcaact accacttgtc agtggatgta
    28741 tgggaagatg tgagtattgt tatttgaaca cacaattagg tgataaacca tttataaggg
    28801 tgtttgtaaa tgtagatgaa atacttgaac agacaaaaaa atatattgaa gatagaaagc
    28861 cacaggtaac catatttgaa ggggcagcta catcagaccc agttccagta gagccatata
    28921 ctaattcttt aaagaaaaca atagagtttt ttggaagaga ggaaaaagga agatttagat
    28981 ttgttactaa atacagtgat gtggatagct tattggatgc tgaacataat aatcatacag
    29041 aaatacgatt tagtatcaat actcctagaa ttataaatga acatgagcat tatacagcct
    29101 ctgcaaataa aagaatagag tcagctgtaa aacttgctag ggctggttat cagattggtt
    29161 tcataatagc acctgtgttt ttatatgaag gttggaaaga agagtataaa aatctgatta
    29221 taagtataaa agaagcttta ccaaaggatt ttgataaaaa aattatattt gaagttatat
    29281 ctcatagata tacaactaaa gcaaaaaata gaatactaga gatttatcca gatacactat
    29341 tgccaatgga aaatgaggat agaaagttta aatacggtca atttggatat ggtaagtacg
    29401 tgtatgataa agaacaatta gatgatatga aagagttctt tcttaaggaa ataaatggag
    29461 tatttaaaaa tagtttaata aaatatataa tatagcaaat cttacctaaa gatttgctat
    29521 attttatttt tatacatttt atgtttataa taaagcaatg agatttagca ctatcatata
    29581 agaaaattaa aaagggcact atctatcaag aacatttttt ctagttttga tactataaag
    29641 gataagagta acaaaaatat caggaggagt tatagtatgc atgcatggga gtctattcaa
    29701 attacattgg atttaataga agcaaattta tcagaagaaa tatcaattaa tgaattagca
    29761 aataaagcaa atttatcacc attttactat caaagactat ttaaaagatt ggtaaataag
    29821 actgtaatgg agtatataaa gcttcgtcgt cttgcaagag cttctgaata cttagcagaa
    29881 cataagaatc gtatacttga tgtagcttta aattttggat ttgctagtca tgagacattt
    29941 acacgttcct ttaaaaaagc atatggatta actccagaaa aatatagagc aaatccagta
    30001 aaactcaatc actttattaa gccagaatta attttaaatt atgctatggt tgatgaaggt
    30061 gttccactga ttgcagatga tatagtgatt gaagtcaatc gtaagacatt gagtaatcca
    30121 aggacatttg ttggaataga aatagaagta cctatttgcc agttggtggg cggtgaaaca
    30181 actggaattg caatagtaga ggaattatgg atgaaacttg gaagtcaaag acataatata
    30241 ccaaatcaaa ttccaggtgg aaatgaattt gctgctcttt atatgggaaa tgcaaaagaa
    30301 ggaaattgta tgtatatggc aggtgtagag gtagagtcag gtacatcagt agaaggatat
    30361 agtacttttg agttgccagc taaagaatat ttagtatgtg gatttgaggc agaaaacttt
    30421 aatgagttag ttaattcagc tgtttttaaa gcagataaat ttatggaaag atggatgaag
    30481 aaacataatt taacaactac tgattttgca attgagatgt attaccccac tacacctgaa
    30541 gcagcatatc tggaacattg gattgttccg gttcctattg aacaatagaa aggatgatga
    30601 atatgaattg gcaagatgcg ttttttcaag atactaaacc tacatttgag caaattaatg
    30661 aatttattaa taatccatta tgggacaagt taaataaaac tttggtttct acttataatg
    30721 tagaacctaa gcttgaatac agtaaatgtt caatgcaaag aggttggaat gtaaaatata
    30781 aaaaaagagg taaatcacta tgtactttat atccacaaga aggttatttt aaagctttgg
    30841 ttattgtaag tgaaagcaat agagtagagg ttgatttatt tattaatact tgttgtgatt
    30901 atatcaaaaa aatatataat gaggtaaact tttttaatgg aagtaaatgg ctaatgattc
    30961 aagtagatag tttattggta ttaaacgata tgcttgaact aattaaattt agagcttaac
    31021 atatagaaca ggatgaatat ataagtcatc ctgtttttat attcaatcag ataaattaat
    31081 atatagtcta taatatgaat aatttagtaa atttaaattt actttatctt tctacattct
    31141 ttgcaactaa gtccacatgt gtttttttct tgcatattag tagcacccca attagcaaat
    31201 tgttgtaaaa taggcacaat acttttacct ttacttgtta atgtatattc aacttttgga
    31261 ggaatttcat tatattgttt acgatgaaca ataccagtag actctaaatc ttttaatgat
    31321 tgagctagca tagtatttgt aatgccatcc aattttcttt ttatgctact atatctaatt
    31381 ggattttcaa ctgctaacat acatataata ggtaatttcc acttaccacc gacaatgtct
    31441 aatgcataac ggattggaca ggttaattct tcaacgtttt tagttatatt tttttcagta
    31501 tttatactca ttttaattac tccttagtta tagtagtatc ttttttatac tttatcagaa
    31561 aaaactgcat acttgttttt tctgtatttt atattaaaat tatatcaaag gataatcctt
    31621 aatacaagta tttaaaaggg gtgtaagaat tatgaaagca ttagaatttt tgaacaaggc
    31681 acgagtttat tatttagcaa cagttgatga gaacaatcaa gcacatgtgc gtcctttagg
    31741 atttgttatg gaatacaatg gtaaacttac attttgcaca tctaatcaaa aggatatgtt
    31801 taagcaactt atttttaatc caaaggttga actttgttgt atagaccaaa accttaatac
    31861 tctacgtatt ttaggtgaag cagtatttgt tacatcaaaa gaaacacaag ctaaggcact
    31921 tgaaataatg ccaagtttag gtcagatgta ctctgtagga gatggtaagt ttgaaatatt
    31981 ctctattgat aaggcaaaag ttagttgttg ttcaatgagt ggtaaaaaga ttgatgtaga
    32041 gctttaatta taattgaatc gacatccata gtgaaaatta agaaaacgtt attactgaaa
    32101 tgttgataga tttaatttaa atgcaataaa aagattttta aataatgaat atgtaaaaat
    32161 ttttagttat tagatgtgta tttcaataat gatatactat tttgaataat tacatactag
    32221 aaataagata attaaaattt actttataat gggatagatt tcttttaaaa aatattttaa
    32281 ctataattga tttttattta atctgaaaaa aatattatga ctataaaaaa ttgctactta
    32341 ttatttggat atttttatat ataaataata agtagcatga ctttatacat taataatgat
    32401 agaatataaa ttaatagata agatatgatt ttaaaaggag tgatataaga tgagtaaata
    32461 tagttcttta tgggaatatt ttaagaaaaa caatagtcaa tcgtttaaat taacttttga
    32521 agaaataaaa gatattgctg ggatagagat agaccattcg tttttgaaat ataaaaagga
    32581 attaaatgaa tatggatatc aagtaggaaa aatctcttta aaagagaaga ctgttatttt
    32641 cgataaaatt ggttaagaca taaataatta catataataa aataatttgt attgatgatt
    32701 attacaaata aaaaagggcg tagcactatt ttttagtgtc acagcccctt ttttgtttat
    32761 aattatgtac atttcacatt atcacgatat aagcattttt cttatgagaa aattaattag
    32821 aattattttt attcatattt aaattattta aattgttttc ccagtttttt atctcctttt
    32881 taacttcttc acttaaagta tcacttgaag atattttctt taatttatca ttcaaagatt
    32941 tcttttgttc ctctgaaagg tctgttttta ttgtaggatc aataatatct attacatctt
    33001 ttacatcaaa atcaacaaat ttgcacagtt cttctagatg atcaattaga aaatctagac
    33061 cttcatatga ccattcttta taatcttttc cgttttttaa tagaaactca ttcacttggt
    33121 aattctgatt tgctaattca aagaatgaac ctgaaggatc cattatatca tttatagagt
    33181 tttcatagtt aacaatatta atttgtctta ttaatttctc tttttctgtt aagttgctat
    33241 aattcttgtt aacaaaagct actaatccaa attcaaagtt taaaattgaa tctttacgtt
    33301 tttttactga atcttctcca aattccctaa gaccttctaa atttttatca atggctttat
    33361 ctctaatctc tattaattgt tctatactta aaccttctaa gtttttaggt actatttctt
    33421 cattattctt agtaacttct ttcaatttat tgttagtatt tttattattg aaaataaata
    33481 tagcagttaa taaacttaaa attattacta aaataattaa acttacattt tttttactta
    33541 acatattaaa cctccttttt taaataagta ttgtacttgt gaaaaataaa tttgtttatg
    33601 aagataacat ttactactgt ttgttctaaa gattataata gaatattttt ttgatataat
    33661 ctatattttt cttattaaat tttcttatta gatatttaat atcaatattt tttagataga
    33721 aaagatttta ataactccaa atatttaata agtaaaataa aattattgct ttaaaatata
    33781 tttttatatg ttttaagttt tataataagt aatattgtat gataaattaa atttaaatat
    33841 ttagagataa gctattatag aatttgctta gtttaagtta aattttaaaa tattcaaaaa
    33901 ggggctagag tctggaaatt tttatttaaa gaatacacat aaaaatttct agactctcat
    33961 catttaaaag aagtattcta aatttattat ttataattaa atattatctg aaaaaatggt
    34021 atttttatac ctttgtttgt tatgcataat tttacttaga acataagctt tatataaaat
    34081 aattaaaaat atacctaatt aaaataagta aaaaaagagc tatcatagaa caaatttaat
    34141 ttgctcatgt gacagctcct ttttagatga tgctttttta gatttatact ataaggtata
    34201 taaaactaag tttattaaaa atgttataat ttctattgct atcatagacc atatcaatcc
    34261 atcaagttca taaaagtaat taagctttat taaatagctt ctttattata atttctttat
    34321 ttcatataat ataaatagta ttaaaatttt tatgggagtt aaagtaagca tgtattatta
    34381 cttagatatt tgtataatca acatctagta atttaccttt ttggtgaaaa tgattaataa
    34441 gttttttagg ataattcatt attaattcat ctggaaaatt aatttccttt aagatatttt
    34501 cagaatactc atagttacct atgtcaaaac aaatgtgagc atctgaagtc agtatgattt
    34561 tagcaccata ttttttgcaa agtgtagcaa tatacttaca gttatccgag ctaccatttc
    34621 ttgacacgcc tttaatagaa caattattta tttctattaa gatattcttt tctatagcta
    34681 atttaatgat taattcatag tcgattgggt agtttgggtt tccaaggtgt cctaatattt
    34741 caatattatc atgcttattt attgcattta ttaaagcttt agtattattc tctaatgtat
    34801 ttggagaaaa cacagcttcg tgaaatgaca aaataagata atctaagcga ggaattacaa
    34861 aaggctctag gtctatgttt cctttaatat ctagtatatt tgcttcacag cctcttaaaa
    34921 ttataatgtt atttatgatt cttgggatat tttgaatgtt gtgaaagtac catctgtggg
    34981 gagaaccagg catcttagga ccatgttcag atgtacctag tatcttaatt ccattttctt
    35041 tacaataatt aatattttct agtaaagtgc tatagccatg accactgact atagagtggg
    35101 tgtgtaaatc tgataaaaaa ttcatataaa ttcctcctct ataaaagatg agggtaacgt
    35161 aatttatatt ataaaacgaa taggaaagaa aatcaatagt tacataatta gacaaaaata
    35221 ttaaaatatt gtagaattaa gcttaataat aaatgtttaa cagtattact gcttataggg
    35281 gaggttaact atgacacaaa agaagctatc aaggaaagaa aaagttttaa tttgcttgaa
    35341 aaggttaaca gaagaacact tgaactttaa taatggcatt agatttggat ttgatgcaga
    35401 atatatagct actgaagtag gtataaatag aagtaatgct agtaaggaat taaatcaatt
    35461 aataaaagaa agtattatta taaagataaa gggtaagcct gttcagtact tatataaaga
    35521 aagtattgag agtattttag gaaagaaact aaataaaggg atatttgata atattaactc
    35581 tataaacgaa tatagtgaaa atgattctat caaagatgag tatattttag agagtataaa
    35641 aaaagagaac aatattttta atttagtagg atgtgatgga agtttaaggt cccaaataga
    35701 acaggctaaa gcagcagtaa tatatccacc taaggggttg agtacattaa taataggacc
    35761 tacaggagtt ggtaaatcta tatttgcaga atatatgtat aagtatagtt taactataaa
    35821 agaagatagt gataatgcac catttatcat atttaactgt gctgattatt ctgataatca
    35881 gcaattatta ttagcgcagc tttttggata tgtaaaagga gcatttactg gagctgataa
    35941 agataagtat ggtattgtac atgaagctaa taatggagta ttgtttttag atgaagttca
    36001 tagattatct gcagagggac aagaaatgct atttctgctt atggataaag ggatatatag
    36061 aaggttagga gaggctaata aggtacacga gtgtagagtt atgataattg cagccactac
    36121 agaagaccca gaaacagcaa tgttggaaac gtttttaaga agaatacctg taactataaa
    36181 gattccgtct ttagaggaaa gaggctttca agagagaatg aagcttatat gttatttttt
    36241 taaagaagag agtgttagga ttggtgttaa gttaaaggtt tcaaaagaag ttattaaggc
    36301 atttatttta tataagtgta agggaaacat aggccaatta aaaagcgata tacaattaat
    36361 ttgtgcaaga gcttttttag attatatgac ctataaaagg gaatgtgtat atgtaaggct
    36421 ctctcttcta ccaaataata ttagagaatc attatatgga aataatagaa aagaggagtt
    36481 tgtacaaaat ttcagtttta ttggaaaaga tgatattatt tttttaccag aagaaaagga
    36541 ttttgaaagt gaaaatttat taatagaaaa taaaaaatat gatttagatt tttatggaat
    36601 gataaaagaa acttggaata aacttcaaca agaaggtatt aaagagtcac aaattagaag
    36661 tgttatagat ggagacatac agaagtattc ctataattta atgaatactt ttatatataa
    36721 tagtagtaat caaacagctt ataacaacat tgttaatgat tcaattgcaa ctactgtaaa
    36781 atatatttta caaaagcatg ataaatggag caaaagagaa ggattagata aattaataaa
    36841 agctatagct ttacatattc agaatttaat tgaaagaata aagattggaa atatagtaaa
    36901 gcatcctaat aaagatgaaa ttatgaagga aagagcatat gaatataaca tagcaaaaga
    36961 aatattatct aacatgtcat taatgtatgg agtagagttt ccagaagatg aagcaatttt
    37021 tttagcaaca tttctttatt tatcgtatgt gggattaaat gaggagagta tagcaatatt
    37081 agtcataatg catggtgaat ctacagcatc tagcatggtg aatgtagcca atactttact
    37141 ggattgtaac catgcagtgg caattaatat gggattagaa gatagagttc aggatatttt
    37201 agtacaagct gtagaaatag ccaataaaat tgataaagga aagggaattt taattttaac
    37261 tgatatgggg tctattttaa catttgctaa agttattcgt gatgctacag gaaaagaagt
    37321 aaaagcaata gatatggtaa gtacacctat tgtaatagaa gcaactagaa aggctctaac
    37381 tccagaaatg acattagaga aattatattt taatattata gaatctataa agaaacatta
    37441 tggagatatg gaagttacat atgctgaaaa agataatggt ggtagatatt ttgatagatt
    37501 actaattgat aatattagta aaactttgac ttttttaaat ggggaaaagg cttattttat
    37561 attaaaagaa gtaattaata gaattagtga acactattct ttagtaattc aagatgaact
    37621 tttagtaaag tttatatttc attgttcatg tatgattgaa agagtaataa taaaagagtc
    37681 tttagtttat aaatcatatg aagaaagaat tagtagaaat aaagaattat atttagttat
    37741 aaaagaaagt tttaagctag tagaagagac attcgatata attatttcag atatggaata
    37801 tgcaaatata ttagatatat ttgaatcaca atatgataca gataaattca aaagttgata
    37861 caaaatttaa aaatagccaa aaggctatct tttttttact ttttttgata cagatttgat
    37921 acaggttgaa aaaatctttt gatacatatc gaaataataa gtttttaaaa aaaattgagt
    37981 aaaaagtttt gattaaatcc tgtaaaatag ccatttgtta ttgatacaca taagttggca
    38041 cggcaattgc ttatataaaa agtgacaaga aaagtgaggt gaaattggtg ataggaataa
    38101 tagttatgac tcatgggagt tttagtgaag aaattataaa aagctgtgaa cttattgcag
    38161 gaccagcaga aagaacagca gcaattaaac taaataggaa tgataatatt gaagacttaa
    38221 ataaaaattt tgtagaaaag ttaaatgaat tagatgaagg tgacggagtt ttagtattag
    38281 ctgacctatt aggaggttcg ccaagtaatg ttgcaagttt aaaccttaaa aaaggtggca
    38341 agtttcatgc acttactgga gtaaatcttc caatgctact tgaagcttta attaatagag
    38401 agggaaaaag tttagaagag ttagcagaag catgtattga agcaggacaa actggtataa
    38461 acaatattaa taaagttctt agctcaatgt aatagaaaaa ggagtttgat taaaaatgtc
    38521 tttagtaaaa tattgcagat gtgatgatag attaatacat ggtcaagtta tatataaatg
    38581 ggttaagcat ctaggtgtaa aaaagatagt agtagtagat gatgaaacaa ccaatgatgt
    38641 tattgctaaa ggtttgataa aaatggcagc accaaagaat attgatttat caatattaac
    38701 tgtaagtgaa tcaaggagat atttttataa taatcaagct gatgataacg tttttgtatt
    38761 aattaaaaat ttagatactg caaatagaat gatagaagaa gggattaata tcaaaaaact
    38821 tattgttgga agaataccta caggaatagg aaaaaagaaa atatctcaaa atgtatacat
    38881 taataaaaaa gagtttcttt taatagatga atttattaaa aagaatatta atgtatcaat
    38941 tcaaatggta cctgatgaag aagaagtaga attaaaccaa aatattaatc gctataaagg
    39001 agagtttatt tagcaatgaa taaatataag gtaatcatac tgtgccatgg aacttgggga
    39061 aaagtcttag tagaagaaac aaataaaaac tttggattgt caaatcaata tgaagtttta
    39121 tcactaagtt ctgaaaaaga tgtttctgta tttatgaaag aggttgaaga agtagtagat
    39181 aaagaagatg tgaaaattat aatatcagat ttatatggag gatctacatc aagtgttgca
    39241 atagctgttg ctataagaag aggaatagat gcaataaatg gacttagctt acagacaatt
    39301 ttaatcgtag atgaagaatt aagcaaagaa gataaattac atacattacc agaaagaatc
    39361 gtcaaaaaaa ataataacct ttgtgtggat ttaattaaag aatttaaaaa aatataaatt
    39421 atgaggtgaa tataatggca aaaataacat tagtaagagt agaccacaga ttaattcatg
    39481 ggcaagttat aacaaaatgg gtaaaaatcg ctcaagctca aaaaataata atagttgatg
    39541 acttcttagg tcaagatgaa tttatggctg acatatataa aatggcagca ccatcaggag
    39601 tagaagtagt tattttaaca gcagaagatg cgggacaagc attccaaaac aacactttag
    39661 gagataaaaa tatatttatt ctatttaaaa atgttgatat ggcaaataag gcttataaag
    39721 ctggacttaa atatgaaaag attcaattag gaggtattcc aaatgaagca ggaaagaaaa
    39781 tggtgtttac agctgtttca ttaggaaatg aggatgtaga acaacttaat gaacttaatg
    39841 aaaatggggt tgaaatagtc ctacaagtta taccagagga atcatctatg acatatgaaa
    39901 atgcgttaaa gaaatttaaa taaaaaatat aaattaggag gaattataaa tggaatctgt
    39961 tttgatgttg gcaattgtaa caggattatg gtattggttt gcagcaggtc ttgcagggta
    40021 tacccttttc tcaacactaa agtctccttt atttataggt tttagccttg gattactttg
    40081 gggtgatgta acaactggta tgattgttgg tgcaagtata gaaatggttt atctaggaat
    40141 ggtcgcagca gggggaaata ttccatcaga taaatgtcta gctgctctta ttgcaatacc
    40201 agtagcactt caaactggtg ttaatgcaga agttgcagta tctattgcgg tacctttagg
    40261 tgttatagga gtattagtta ataacttaag aagaactggt aatgctgtat tggttcataa
    40321 ggcagataag tatgctgaag aaggtaatac aaaaggcatc tggagatgtg caacacttta
    40381 ttctttaata tttgggtttg ttttaaggtt tccaatagtt tttgtatgta acttttttgg
    40441 agcagattta gttcaatcat tattagatgt tataccacaa tggttaatga atggattaac
    40501 agtaatgggc ggtatattac ctgcattagg ttttgcaaca acaatattta ctataggtaa
    40561 aaataaattc ttaccaatgt ttattatagg attttttatg gttcaatatt ttgaaatatc
    40621 aattactgca gctgcaatat ttggtgtttg tatagctctt ttaattacct ttatgaaaga
    40681 agacaaaagg gtaggtgaag tataatgcaa gaaataaaaa ataaagaagt taaaaatgga
    40741 caaggtccaa ctgaagttaa taagacaaga gtacttacaa agaaagatgt aacaaaaaca
    40801 tatctaagat ggtggtggac agcagaatta tccaactctt ttgaaagaat gcaagcatta
    40861 gcagtttgtg caagtttcac tcctgcttta gaaaagttat ataagagaaa agaagattta
    40921 gtagatgcgt taaagagaca cttacaattt tttaatactc aagctatatg gggaggatta
    40981 attcacggta cagttttagc tatggaagaa gagaaagcta ctgaaggaaa aatcccagga
    41041 gaagttattt caggtgttaa gaatggtctt atgggacctt tggctggaat tggagatact
    41101 ttagattttg gtacatttca aacaatattc ttagctttag gtgcttcatt tggagcagaa
    41161 ggaagtgtta taggtgcatt tttcccaata atgtttagta tattactatt ttgcgaaggt
    41221 tattacttat ttcatttagg atattcttta ggtagagatt caattaagaa aattctatca
    41281 ggtggtattg tcaataagat aattgatggt gcttcaattc taggaatgtt tatgatggga
    41341 gcattatctg caactacagt taagctttca actccgctat catttgatat tggtggtaaa
    41401 gcaattgttg ttcaagatac attaaatatg attgctccag gtttattacc acttggtgtt
    41461 gtattcttcg tatattgggg aatgaaatac aagaagtgga caataactaa gttattagta
    41521 attttggttg tattagcatt ggtagggtca ttcattggaa tattctaatt cacagtgatt
    41581 aaaatataga atttaatttt aggaggagaa agaaatgtca gaaaaaatgt atacagaaaa
    41641 ggaatattta gaaggaatta gaggagcaac aggagaaaga gcagtatggt tctatcttct
    41701 tatgaaagaa gctgaaaaat taggagctaa tccagatgat atatgtaaag aagcaatata
    41761 tggatttggt aaaatgagag gtcaaaaata taacgtagca gatactccag gaaaaatggc
    41821 agaaatgtta tataacagta aaggacaaaa agtgtttgaa atggaattag tagaaaatac
    41881 agatgagaat ggcgttttaa aattccatca ctgtccatta gatgctgctt ggaaagaata
    41941 tggattaact aaagaagaaa gaaaagaaat ttgtagatta gcttgttatg gggattatgg
    42001 aagagttgac tgtgcacaag gtgttaaatt agattttgct caaaaatgtg ctcatgatga
    42061 tgaagtttgt gaattagtat ttacaaaaaa ataattttta atgctaaaag cctgttcttt
    42121 aaggacaggc tttaaaaaga tagaaagtag gttgatttat ggatataagc agaagtgtag
    42181 ataatagttt aaatagaacc atagaatttc taaaagaatt gataaaaatt gattcacaac
    42241 aaggtgaacc tatttcacaa tgtccttttg gtataggacc taaaaaatct ttagataaaa
    42301 ctttagatta ctgtgctagt ttaggtttta gtgttaaaaa tattgataat tatattggat
    42361 atgcagaaat tggagaggga gaggaattaa tagggattcc aatgcattta gatatagttc
    42421 caccaggaga ggggtggagt gtagacccat tttcgggagc agtaattgac aatattattt
    42481 atggaagagg tgtcatagat aataagggtg ctgtatctat gcttatacat gtattgaaaa
    42541 acatagagga tatgtatcct acaattaata aaagaataag attaattttt ggtacaaatg
    42601 aggagacagg tatgaaatgt atcaaatatt acttagataa aggtgaagaa attccaagta
    42661 tgggatttac tccagatgct atgtatccag tggttaatgg agaaaaagga agggttcata
    42721 taaggataga aaaagaaatt aaaattgaca aaagtaaacc ttacataata gtaagtggag
    42781 gaactaaaga aaatgttgtt ccatctcatt gtactgcaaa aattattaat ggaattattt
    42841 cagagttaac tactaaagga gtagctgtac atgcaagtaa tcctgaaaag ggagaaaatg
    42901 ctatatctaa aatggtaata aagatagttg aggataatat ggattttcag catagagaag
    42961 atatagaatt agtttcaaaa tacctttgta gtgattatta tggagatgca ttggggatta
    43021 atcaatatga tgaggtattc aaaaacacta ccttaaattt aggaatatta aaagtaaatg
    43081 aggaaaaaat cgtatgtgag ttagatataa ggtatggtaa aaatatagtt ttaaacaata
    43141 taatagatag attcaaaaag gttttttgta atggttggaa aatagaggtt attgttcata
    43201 aagacttaca ttatgtggat gagagtaatt tagtacttaa aaaattatta gaagcctatg
    43261 aagaagtaac agatgaaaat ggatatacaa ttgctatggg tggtggcaca tatgcaagtt
    43321 ggtttaaaga tatggtggcg tttggaccta agttcttagc gtataaaact ggtggtcatg
    43381 gagtagatga aagagtacca ataaatcata tacgaaagaa tatggagata tatactttgg
    43441 ctttaataaa actattagaa ttgtaaaaga agaagagact ttatgtgcaa atttacagat
    43501 atcttaaaaa gagagataaa acgagttcaa ccttatttaa atgttcaaca ccatcaaatg
    43561 gggctgtctc aaaatagaga taagcctctt acttattgtt gaaatgatga aaatatattt
    43621 tacttcatac aaaaaagagt gccttatgaa gtaaaaagtt aattttgaga caccctcatt
    43681 tgatgatgtt gaagagaaaa taagtatgaa attattaaaa aagatgattt tataaaagtt
    43741 aaggttgaaa agaaaagtta attaaatgga attagataaa tggagaagga tgtatactat
    43801 ctaataaaat aacaaaagag tgttataaag ttggttatat gattagagaa aagtctatcg
    43861 atatttatcc agatagtgga tggggattta tgacttggga tgaaaatgga gaataataaa
    43921 aattatagtt tatacagttt aataagatat atgtacaagc agttaatcta aatgattgac
    43981 tgcttttctt ttatagattt ttatataata gacaaatacc aatacatgat agttgcctgt
    44041 tataattaga ggaataaata cgaataataa ctttataaaa ggattaaatg gaaaatcgaa
    44101 gagttatatt cagtatttta aaaagaatat gaaatgagaa atgaaaataa ggtgtttata
    44161 ttaaaagagg ttttacacaa aatatttaaa aacgtgataa ataaaaactg tatttagata
    44221 gattaactat tagttttata taatatggat tttatttaca aagtttacat attttacaaa
    44281 ggagattaaa gataaattta aatatatatt ttatatactt gtttatttgt ttctctgtgg
    44341 ttgatttttt cgtcaaaagt caacttcaaa tcgcttgtta gctttacatg tttaaggata
    44401 taattataca taaggaggga ttgacatgaa tgaattaaac atcgcaaaaa cgctgatttt
    44461 aaagcgtaaa gaaaaaggaa ttacacaaga tgaacttgca aattatatag gcgtttctaa
    44521 agcttctgta tctaaatggg aaactggaca aagctatcct gacataacct ttttaccgca
    44581 actcgcgaca tatttcaaca ttaccgttga tgagttaata tgttatgaac ctcaaatgat
    44641 gaaggaagat attaataaac tgtataataa actgtgcaaa gactttacgg ttaaaccatt
    44701 tgatgaagtc atggatgaaa taagagaaat cataaaaaaa tattattcat gttttccttt
    44761 gatttttcgg atgggtcttt taatagttaa tcactatgat attgtagatg aaaaaaagcg
    44821 tgaactatta attagtgaag cattagaaat atttattcgt atacaggaaa cttgtaatga
    44881 catagatatt tgccgtcaag caaaaagtat ggaggcaact tgttatatat tgttaaatca
    44941 acccatacaa gtgattgatt tattgcaaaa cagcaatttt cccatgataa atgaatctat
    45001 acttcttgca caagggcaaa tgatgaatgg tcaaatggat gaagcaagag aaacatttca
    45061 acttggtgca tatcagaacc ttatatcatt agtccaaaat ctagtgggta ttttgcaaaa
    45121 tgcagataaa ttacaaatga aggaaataga aagacgtatt ctagcaattt cagatatatt
    45181 tgagcttgat acattatctc ctgctattat gctttcttca tatttaacac aggcacaaat
    45241 taatttaata catggtgata atgaaggagc tattaaatca ttacgaaaat atgtagattt
    45301 agcaacacgt gatatatacc caattataat acatggggat gactttttta ataaacttga
    45361 cagatggatt tctgaaattg gaacaggtat tacacgtgat gatacaatcg taaaagcagg
    45421 tattgttgct gctattaaaa ataatccaat gttttctgta ttaagtgaaa acaaagaata
    45481 caaatttctt atagaaaaat tatctttatt ggaggaataa gattatggaa aatttgatgg
    45541 aatacttacc acttttggtt cctgttatta ttttagattt aatacttata attactgcac
    45601 ttgttcatgt gttgagacat ccaaattata aaattggaaa taaggcaata tggattattg
    45661 tagtactgtt tattagttta ataggaccaa ttttgtattt tacaatagga agaggtgaag
    45721 aatgatgaat attgtctcaa taaatggatt atcaaaaggt tttggtaatc gaaagataat
    45781 agataattta aattttactg taccagaagg gtcagtcttt gggtttgtag gtaagaatgg
    45841 tgcaggtaag actacaacaa tgaaaatggt acttgggtta cttaaaccag atagtggaac
    45901 tatagatgtt tgtggtgaga aagtgaccta tgggaaaaca agttctaatc gtcatgtagg
    45961 ttatctgcct gatgttccag agttttacaa ttatatgaga ccacttgaat atctttctct
    46021 ttgtggtgaa attacgggac tttcaaaaaa agaaatacag ataaggagtg aagagttgtt
    46081 atcacttgtg ggtcttagaa atgaaaagag acgaataggt gggttttctc gtggaatgaa
    46141 gcaacgactt ggtatagctc aagctttgct ttcacgcccc aaattactta tttgtgatga
    46201 accaacaagt gcacttgacc ctgttggtcg aaaagaaata ttagatatta tgcttaaaat
    46261 caaagactca acaactgtaa tattttcaac acatatacta tcagatgttg aaagaatctg
    46321 tgaccatgtt gctatattaa acaaaggaag cattgccctt agtggtacac tttcagaaat
    46381 aaaaagtatg cacggaaagg atagactttt actggagttt gcaagtaatg atgaaattca
    46441 aaagtttaag tctagtgatg gtattaaatc cttactaaaa gattcagaag aaactaatat
    46501 ggaaattgtt ttgcatggta aagatataaa agcaatacaa aaaacagtta tatctacgct
    46561 cgcagaaatg aatctttgtc cagtaaagat ggaactaata gaattatcac ttgaaaattt
    46621 atttttggag gtagtaaagt gagagaatac atagcattta caaaaaaaga atttaaagag
    46681 aatttaagaa attataaatt atttagttta attattttat ttctaatatt tggtataagt
    46741 agtcctattt ctgcaaaatt tatgccagac ttgattgctc attttgcacc gactttaaaa
    46801 gttactgctg caccaactgc tttagattct tggacacaat tttttagcaa tatctctggt
    46861 cttggtatga gtttaactct aattattttt tgtaatattt tatcaaatga atattctaaa
    46921 gggacacttg ttattatgct gactaaaggt ttatcacgtt catctatagt attgtctaaa
    46981 ttctctgtta cggtgattat tatgacaatt ggtttttggt tgagtttttt atttgcttat
    47041 ggctatacaa tgtatttttg gccaactgca aatcttaatc atattatatt ttcagcattt
    47101 aatttatggc ttataggaat tatgtatatt agtgtattaa ttcttgggtg tgtactattt
    47161 agaccagcat ttgccagtgt tttacttgta ttggtagcaa ctctcttatt gagtttaatt
    47221 tctattccta aacagattgc accttatact cctaatttct tgatgtcaaa aaatatagac
    47281 ttgatttcag ggaaagtggc aatgccagaa tttattattc caattattat aacagttctt
    47341 atttctatag tatgtttact agcagctgtt attttattta ataaaaaacc agtttaataa
    47401 tacctttatc acaaggtaaa gtaatcttat gttgttaaaa actacaccta aaaatcaaaa
    47461 agacttggac ttaagtttca agtctttttg atttttaggt gtagtttaat atatagaatt
    47521 aagtgtagaa aataatacaa ataatattat ttgtatcaaa taaatataag aatatttaat
    47581 tatgttaaaa tatactaaaa attactttaa aagtgataaa attagtataa gtaaatattt
    47641 taaaggggtg tagatatgga aaagtcaaaa gtatattttt gtgatttata ctcaaattcg
    47701 caaaataaaa acgtaccaaa caatgtaaga agattatttg atgaagcagg attcaaagat
    47761 ttaatagaaa aaaatgacca agtcgcaatt aaacttcact ttggagaaaa gggaaatact
    47821 acatatatga gtcctgtggc agttagacaa gtggttgata aggttaaaga ctgcgaagga
    47881 aagccatttt taacagatac aaatacactg tatacaggga gtagaacaaa ttctgtagac
    47941 catcttacaa ctgctataga aaatggattt gcttatgcag ttgtaaatgc accagtaata
    48001 attgcagatg gattatatag tagaaattat gagaatgtaa aaatagacaa aaaacatttt
    48061 gaaagtgtaa aaataggtgg agaaatttat aattcttctg caatgatagt aatgagtcat
    48121 tttaaaggtc atgaagcagc aggttttgga ggagcattaa aaaacttagc tatgggatgt
    48181 gcaagtgcag caggaaaaca aatgcaacat tctgatgtta ctccagtagt aaaggaaaag
    48241 aagtgtgtag gttgtggaaa gtgtgttaat tcttgtccta caaaagctat aagtatagtg
    48301 gataaaaaag cagttataga ttctgatgta tgttatggat gtggagaatg tccaactgtc
    48361 tgtccaacta gagcagtaac tatacaatgg gaaagtgact cagatgtgtt tgtcgaaaag
    48421 atggcagaat acgcttatgg agcagtatca aataaaaaag acaaggtagg gtatataact
    48481 tttgttatga atgtaactcc actttgtgat tgtgtacctt ggtcaggtag accaattgcc
    48541 catgatatag gaatattagc atcaacagac cctgtagcta ttgaacaagc atgctatgat
    48601 ttgatttgta aggaaatggg tcatgatgtt ttcaaacatg aacatcctca tgtaaatgga
    48661 actagaataa tagactatgc atgtgaaatg ggtatgggaa gtaaagaata tgaattaata
    48721 aaattataag aatagtagca cttaaatgca tataattatt gtaagagaaa tctaaaagta
    48781 tcaaaggagt atgcatttat gaaaatttac tatgagaagg aaaacagcaa gaaggctaat
    48841 gtaaacaata ttccaagtgt atttgcgttt aattttctaa gaaacttttt gactagcaaa
    48901 gagtatgagg aaactagaaa agaatatttt ataaataatc taacaaaaag tcaggttaat
    48961 caagccatga aggatttaaa atggctattt agagaatatg aaggcttaga agttattaca
    49021 atggaaagct tggatggagt taagacaagg atagttttat aaaccagtaa gaattatatt
    49081 ctaataaaaa atactgcttt aataataata tgtataatct atatactaat attgttttga
    49141 agcagtattt ttttgtgtat agtaaacttg tgtctaaaat aaagttgacc aataaatgtt
    49201 atatatgcaa tactatatat aataagtaaa ctacataaaa atattaagtt gacaaatagg
    49261 aggatatatg gaaaaataca taataaaatt aaaaaataaa gtattgagag aaaaagaaat
    49321 aagttatgaa gaagctctta atttaataag ccttgataca aataataaaa acgattttga
    49381 tattctatta aaatctgcaa atgaaataag agaatatttt atgggaagaa aagcagattt
    49441 atgtacgata atgaatgcta aatctggcaa atgtagtgaa gactgtaaat tttgtgctca
    49501 gtcttcttat tataaaactg gtgtagaaga atattcatta ttagattaca atgaaatctt
    49561 aaatagagct aaagagatgg agagtaaagg agtgcataga ttctcattgg ttacaagtgg
    49621 taaaggtatg tcaggaaagg agtttaataa tattttaaat atctatgaag gattgaggga
    49681 aaatactaat ttaaaattat gtgcatcttt aggtattata gattatgagc aagctaaaat
    49741 gttgaagtca gcaggtgtga ctacatatca ccacaatgtt gagacgtgta gagataattt
    49801 tcataatata tgtacaacac acacatataa agataggata aaaactataa aagatgctaa
    49861 aaaagctggg ctagatgtat gtgtaggcgg tataattgga atgaatgaga gtgaagaaca
    49921 gagactaaaa atggcttttg aaatacgaga attaaatgta aaatcatttc caataaacat
    49981 tttaaatcct attaagaata caccaatgga aaattatgat gtattagaac caatggaaat
    50041 actaaaaaca acagcagtat ttaggtttat aattcctaat gtgtacataa ggtatgctgg
    50101 tggaaggcta tcattaaagg gatatgacaa agttggattt aatgggggag taaattctgc
    50161 aattgttggt gattatttga caactgttgg aagtgggatt gaaaatgata aaaaaatgat
    50221 tatagagcaa ggatttgaac tttaataaat actgcttaaa ttaattaata tacttttaga
    50281 taattataaa agtatattaa tttttttttc agattaattt aaatattaaa attgaattat
    50341 aagttgcaat attttgaaaa aagggaaaaa tatcaaaatt tatataaaat ggttgcaaat
    50401 ggaaaaaaga tgtgatagta tctaggtaac cggttaagta aaacggtttc ctaaattttg
    50461 ggggtgaact tcttgagtat aactataaaa gaaattggcg aattggctgg tgtttctaaa
    50521 acaactgtgt caaaagtcat taataataaa gatgaaaata taagtcaggc aacaagagaa
    50581 aaaattttga aaataatgaa ggaaaaaaat tatgtaccaa ataaattagc acaaagttta
    50641 gtaactaaaa aaactaatac tatagggctt cttataccag atatacgaaa tccattcttt
    50701 acagatgtat caagaggtgt agaagacaaa gctaatgaag aaggctacaa tataatttta
    50761 tgtaatacag atgaagatgc taaaaaagaa tatgaaggaa taagaacact atcagagagg
    50821 atgatagatg gaattatatt tgcagcatct tcaaatacaa actggaaaga agcaaattat
    50881 aaagatataa aaataccaac tgtcttgatt gataagaaga taagtatgaa caaagaaacc
    50941 ttaaaaggaa ttgttaaaat aaataacttt gaaggggcct atatagctac aaaacattta
    51001 ttagatattg gaaataagaa aataatatac ctaagtggtc cacttcaaaa tgaaatagca
    51061 gtagatagat tagaaggata taaaaaggct ctaatcgagt gcaatttaag ctacaatcct
    51121 gcttatgtat ttgaaggaaa atataagatt gaatggggac aagaatttat aaaaaattta
    51181 gaaaagatag attttgatgc aatattttgt gcaaatgatt tgattgcaat aggtgtcata
    51241 agaggtctta aagaaagagg attaagtata ccaaatgata taagtgtcgt gggatttgat
    51301 gatattcaaa catcaagtct aataagccct tctcttacca cagtaaaaca gccgtcttat
    51361 gatataggat ataaagccag tgaaatattg ataaattgtt taagaggaga taaaaaagaa
    51421 tcgtttgatg aattgatttt caaaccagaa ttggtcataa gagattcaac aaaagaaagt
    51481 gaataacagc acgagagaca taaatagata tgtaaaggag taatgtaatg ggaaacatag
    51541 ttgtcatagg aagtgtaaac atggacatgg tatgttctgt agataaaaga ccagaaaaag
    51601 gagaaacagt attaggtaat agttttttta catcacctgg aggaaaaggg gctaatcaag
    51661 ctatctcagc ttctaaacta ggagcaaatg taaaaatgat atcatgcata ggggaagatg
    51721 gcttaggaga ggagttaata agaaatttta gaacgaataa agttgattat agcttagtat
    51781 ccagaaataa tcacaaaagt tctggtgttg ctgttataac attatgtgaa aatgataata
    51841 gtattgttgt tgtaccaggg actaatgagc tagtagatat agaattaatt aaaaagaatg
    51901 aagaagagat aaagaatgca gatatagtat tgctacaatt agagattcca ttaaaaacaa
    51961 taaattatgt agtgaatttc tgttttgaaa ataggattag ggttttatta aatccagcac
    52021 cagcagtaaa actaaatgaa gatataatag aaaaagtaac ttacttaaca ccaaatgaac
    52081 atgaatataa gatagttttt gacacaaatg aagggataga agaagtatta aaaaaatatc
    52141 caaataaact tgtaataaca gaaggaaaaa atggagctag attttatgat ggtgaggaaa
    52201 tcaagcatgt atcttgtata agtgttgatg ttcaagatac cacaggagca ggagatacat
    52261 ttaatggagc attggcagtg gctataacag aagggaaaaa tttatataca gcagtagaat
    52321 atgcagtagt agtatcaggt ctgtctgtaa ctaaactagg tgcacaatct ggtatgcctt
    52381 ataaagaaga tgttgaaaaa tatttaaata ataaataaaa atctaatttg ttcaaattta
    52441 aaactattaa aaatggaggt taattatgag agtgagaaaa attttatctt taggaattgc
    52501 tggaatacta gcaataggaa tgttaacagg gtgttcaatg gaaggaccaa gtaaatcaga
    52561 taataaaggt ggttcagaca agaaagattt aactattgga gtatctacaa taactcttca
    52621 acatcaattt tttatagata ttgatgaggg aataaaagaa aaagctaaag aattaggtgt
    52681 gaaagttata gtaaatgacc cagaccaaga tgtagctaag caaacatcag caatagaaga
    52741 ttttatacaa caaaatgtag atggaatgat agtacttgga actgataatt cggctatagt
    52801 accagcagta gaaggagcat ttgaaaaaat gccagtagtt acagtagatg ctgtattaaa
    52861 tactgaaaat attacaagtt atgtaggaac agttagttat gatgctggta aaaaattagg
    52921 ggaatacact aaaaaataca tagatgaaaa gttaggtgga aagtcagaaa tagcaatagt
    52981 gacagattta aaatctcaaa tacaaatgca gagaatagat ggatttaaag atgcattaaa
    53041 aggttcagca aatgttaaga tattaaattc acaaccagga tatgacagag aagaatctct
    53101 taatacagta gaaaacttaa ttcaatctaa tccagatgta gatataatat atgcaacagc
    53161 tgaaaatagt gtactaggag ctaaagctgc acttgaatct gcaaaaaata aggatgttaa
    53221 gattgtagga tttgacttaa ctgaagaagc atcaagtgga ataacagatg gaactattct
    53281 tgctatgatt caacaacaac ctaaagagat gggacgttta gctgtagaag cagtagtaaa
    53341 agctataaaa ggggaaaagg tagaaaagaa tataccagta ccagctttac tttatgataa
    53401 ggaaaatata aaagacttta agaattagta aaataaaatg agattatttg aaagaaatcc
    53461 tatttaaaaa ggagtgtcat tatgagtaat ataattctga agttaagtaa tattgctaaa
    53521 gaatttcctg gtgtcagagc attagataat gttaattttg agttatttca tggcgaagtt
    53581 catgcactac tgggtgaaaa tggtgctggt aaatcaacaa tgataaagat tttaactggt
    53641 gcacattcga aaacttctgg aaaatttatt tttgaaggca aagaaataga gaatatatcc
    53701 ccagatatct ctaaaaagat aggtataaat gccatatatc aagaactaac agtttttgac
    53761 gaattaacag tagcacaaaa tatttttatg ggaaaagaaa taaatggcaa agtcttaacc
    53821 aatgacaaaa aaatgaatga agaggctaaa aagattttcg ataatatggg aatagatata
    53881 aatccaaata gtttagtcaa agagcttagt atagctcaaa aacaaatggt agaaatagca
    53941 agagttttat catcagaaac taaagtatta ataatggatg aacctacttc ttcaataagt
    54001 aaaaaagaga ctgaaatact ttttagactt ataaatgatt taaaagagag tggagttagt
    54061 ataatatata tatcccatag gatggaagaa ctctttgaga tttgtgacag aataactata
    54121 atgcgagatg ggaaaacgat ttcgacttta aataccaaag atgtatcgtc tgaggaagaa
    54181 ttagttaatt taatgataga tagaaagcta gaccaatttt tcccaaaaag aaaagtagaa
    54241 ataaaagaag aaattatgcg agtggaaaat ctaactaaga acaatgtttt taatgatatt
    54301 agctttaata taagaaaagg tgaaatattg ggaataggtg gactagttgg aagtaagaga
    54361 agtgaaatag tagaagctat atttggactt agaacatatg attctggaaa aatatattta
    54421 aacaatgaag aagtaaagtt taaaactcca tcagatgcaa tagaaaatgg actaggatta
    54481 ataacagaag atagaaaagg tactggattg tttttacaaa tgagtgtcaa agaaaataca
    54541 actatggctg ggttaaaaaa gatatctaag tttaaatcaa ttatagatag aaaaaaggaa
    54601 aaagagattt tagaaaaata catagaagct ttaaagataa aaacaccaca tatgaatcaa
    54661 gttatacaaa gtttaagtgg tggaaaccaa caaaaagcta taatagcaag atggctttta
    54721 ttacaaccag atatattgat tatggatgaa ccaaccagag gtatagatgt aaatgctaag
    54781 gcagagatat acaatctaat gggagattta gtggagagtg gtgtaagtat aataatgata
    54841 tcttctgaaa taccagaatt aatatcaatg agtgacagaa taatggttat gagagaaggt
    54901 catataagtg gatttttaga aggtgaagaa atggttgaaa ataatgtatt aaaattggct
    54961 ttcggaggta aaattaatga gttcaataac taagcataaa gaagaaaaat cttctccatt
    55021 attgacaaaa ttaacaagtg tatttagaga ccaagggagt gcagctgtag gacttgtaat
    55081 aatttttata ataatgagtg tagcttcatc taacttttta actttagata atttaattaa
    55141 tgttggaaga caaatatcaa taaatgcaat acttgcagta ggtatgacat ttgtaataat
    55201 tacaggagga atagatttaa gtgtaggagc agtaattgct ttagtaggta cattttgggc
    55261 tacaacagta gtgaattata atgcacctat atgggtaggt atgatacttg cattagcaat
    55321 agggacaata ttaggagtca taaaaggtgc aattatttca acacaaaaac tacctccatt
    55381 cattgtaact ttagccatgt taacgataat aagtggggca agctttgtat ttacaggagg
    55441 aagacctata tcagtaaata cagatgcatt taagatgctc ggtagaggat atattggacc
    55501 aataccaata ccagttataa ttatgattat agttgtaata gcaggacatt ttctattaaa
    55561 aagaactaat tttggacgtc atgtacatgc agtaggtgga aatgaagagg ccgctagatt
    55621 atgtggagta aaagtaaata aagttatagt aaaagtatac gcactagctg gactactaac
    55681 agcattagct gggataatac tatcatcacg tttggcatca ggttcaccaa ctgttggaga
    55741 tggagcagaa ttggatgcta tagcagccgt tgtgcttggt ggtactaata tgatgggtgg
    55801 ttctggttct atagtaggta catgtatagg tgtcggaata ataggaatat taggaaatgg
    55861 attaaactta ttaagtgtat cttcatataa tcaaatgatt ataaaaggtt tggtaatgtt
    55921 atttgctata tggataaata atataaaatt aaaaaaatca gctaaatcta aatagtaatt
    55981 aaattttagc ttataaacta taaaaattat taagtttttg tatatatttt tatactttaa
    56041 tttgcataca tctttataca ttgaatgaga atatgctaag tgaaaaagtt tactatggct
    56101 taaatagatg agattagact tgaaatttta aatgtaaaaa taaatcttag gaggaaatat
    56161 gaaaaaaatt ctcttagata cattaaatag taaaaaacaa gaatacatag attatttaaa
    56221 agaacttgta agtataaaga ctgaagatgt tggtcatgga atactaggtg gatttgaaaa
    56281 agaaggacag gaatatattg aaaaacttgc aaattacata ggttttagtg tggatagaca
    56341 agaaatgagc gaagaattaa ttaaaaaagc aaaaaatata tataaagaag gaaatctagg
    56401 tcacaattat caagatagat ataatttaat ttgtaagtat agtgatgatt tacctggtaa
    56461 gacaattgta tttaatggtc atgtagacac tatgccaccg ggtgatataa gtaagtggaa
    56521 atataatcca taccgtgcta cagaagataa tggaaaactt tatggtcttg gaactgctga
    56581 tatgaaatct ggacttatag catcaatatt agctgtaaag cttataaaag atagtggtct
    56641 aaatgtacct ggtaatgtaa aaataatgtc agtagtagat gaagaaggtg gcggaaatgg
    56701 taccataaat gcagttatga atggtattga tggagattgt tgcataattt gtgaaccatc
    56761 agaacaaaac ttaatagttg cacatatggg atttgtattc tttgaagttg aggttaaagg
    56821 tgtgtctctt cactgtggta gtaagtggga gggtgtaaat gcaattgaga aggctatgtt
    56881 gttgcttcaa gatataaaag agttggaaca taactggctt atgatatata aacatccact
    56941 attaccttca cctacaataa atttaggtgt aataaatggt ggaacagcag gttctactgt
    57001 gccagataag tgtgtattta atttatgtgt acacttcttg ccaaatataa tgagttatga
    57061 acaagttgta aatgatgtaa caaatgtcat aatgacaagg gctaatggag atttatggct
    57121 taaagataat aaaccaaata taaacatata tcaatcaggg ttaggttttg agatggacaa
    57181 ggattctgat tttgtagtga atgcacataa aatactagaa gaaactctag gaaaaaaatt
    57241 agagataaaa ggttcaacag ctggcaatga tgcgagagta atgaaaaatt tagctgaaat
    57301 accaacactt atattagggc caggttcaat agaacaatgt cattcaattg atgaatatgt
    57361 tgaaattaag gagtatttag atagtatact tatgtatgca agtctaatat taaacttata
    57421 aaagtaggag gaatatataa atgaaaaaag tattaatagt aggggaatct tgggtaaaaa
    57481 atataactca tataaaaggg tttgatacgt ttgttacaac tcattatgaa gaagctgtaa
    57541 agtggttgaa agaagcaata gaatctggag gatatgagac agttcatatg cctgcacatg
    57601 tagcagcaga tagttttccg tataaattag aagagttaaa tgagtatgac tgtataatat
    57661 tatctgatat aggttctaat acgttcttat tatctaatag tacttttata gattgcaata
    57721 gtaatccaga tagacttgaa ctaatcaaag agtatgtaaa taatggtggg gcgttaatca
    57781 tggtaggtgg atatatgtca tttacaggaa tagatgcaaa agctagattt ggagaaactg
    57841 ctatcaaaga tgtattacca ataacaatga tagataaaga tgatagagtt gaaaaaccag
    57901 caggaataat tccagaagtt attgactctg aacatccagt attaaaaggt ataccaacag
    57961 aatggccaaa gtttttagga tataacaaaa cagtagctag agataattgt cctgttttag
    58021 caactattgg aggagaccca tttgtagctg ttggcgagtt tggaaaagga aaatctgcta
    58081 tctttagttc tgactgtgca cctcactggg gtccaaaaga atttactgat tggaaatact
    58141 ataataaatt atgggttaat atgcttgatt ggttaacatg ctaaaaattt tataaaactt
    58201 gaaaacctct tacttgataa aatgaaactt acaagtcaag aggttttatt gtataaaatg
    58261 gttgtatggt agaaaatatt tttaaatcag ttaaaattag tcattttggc tgataatcga
    58321 taagttatta gaaacaggga ggtttatata attaatatgg ataatttaaa taaaaagttt
    58381 ttgatggaat atatagatga gatacaggga gaattaagag atatatcaga taagatatgg
    58441 aaaaatccag aacttcagta taaagaatac tatgcttcaa atttacaaaa agaatgttta
    58501 aaaaaacatg gttttagtat agagggatta gaaggtatgg atacagcatt tgtggctagt
    58561 tatggagaag gtagaccagt tatagcagtt ttgggagaat atgatgcttt agatggtttg
    58621 tctcaaaaag tatccactac taaagaacct attgtagatg gtggtgcagg tcatggatgt
    58681 ggacataact tacttggtac aggttcaata ggagctgtaa ttgcagtaaa ggaattaatc
    58741 aaactaaaaa agctgaaagg gactataaaa tactatggat gtccagcaga agaagattta
    58801 tctggaaaag tattgatgat aaaaaaaggt ttttttgatg gaattgattg tgcttttagt
    58861 tggcatccat ttgatataaa tactccaata agaataccaa cacttgctaa ttactctgtg
    58921 aaatttagat ataatgggat tagtgcccat gcagcacaag ctccttataa cggaagaagt
    58981 gcacttgatg cagtagaact tatgaatata ggttgtaatt atttgagaga acatatattt
    59041 gataattgta gagtacacta tgtaactact aatggtggaa aaatgccaaa tatagttcct
    59101 gattttgcag aagtgtggta ttacataaga ggagttaaaa tggagcatgt aagagatgta
    59161 tttggtagga ttgttgatat agcaaaagga gcagcattaa tgactggtac tattatggaa
    59221 tatgacatta taagtggagt atatgactat attccaaata caatattaac agatattcta
    59281 tcacaaaata tgaaattagt aggtgtgcaa aactataatg aagatgacta ctattttgca
    59341 gataaattag ctgagacagt gtctatagat aaaagaagta gtgtttctag tgtattaagt
    59401 ggaaataagg atattactaa gatgaatctg catgatgaag ttacggatga tacatttact
    59461 cataataact gtatatcatt atcattagat attggagatg ttagctacat aattcctaca
    59521 gcacagtgtt cttgttcagt ttggcctatt ggtatttctg cccatacatg gcaatcttgt
    59581 gccagtgcag gttctgatat gggatttaag gcaatgttat tggcatctaa atctatatca
    59641 tgttcaataa atgatgtaat gcttgatgaa ttggttataa acaaagcaaa aaaagaattg
    59701 aaggatactg taggtagttt taaatatata ccaattattt aaaacttaaa ttgatataag
    59761 cataactaac ttgtatgagt taatataagt atataattta atattatttt taacccaacc
    59821 ttctaattat tttaaattta agatacaatg attagaagga attttttaag tttcttagaa
    59881 tactgtaaat tatataatct tgataaaaag tgttatacta tatataatct aaataaaaaa
    59941 tagtacaaaa aggagtattt atgtttggat atgttaaaat aaacaaaatg gatttaacat
    60001 ttagagaata tgattattac aaagcttact attgtggact atgtaaatac ttaaaaagaa
    60061 atcatggaga aatctctagg ttttctttga actatgatat aacattttta attgttttat
    60121 taacagcagt atataaccca gagtctataa gtacagagga agtgtgtata gtaaatccat
    60181 ttaaaaagaa aaaggtaatt acaaatgata ttacagaata tgcagcaagt atgaatattc
    60241 ttttaacgta ttataaattg gaagataatt taatggatga taaacgcata aaagataaat
    60301 tggcatatta tatatataaa aacaaattaa aattagcata tgaaaagtat cctgaaaaag
    60361 cagagtatat aaaacaacaa ctaaatgaat taaataagtt agaaaaagat aaaaatataa
    60421 atatagatga agtatctagt atatttggaa atataatggg tgaagtattt gtctataaaa
    60481 aagatgaaaa tgagagaaac ttaagaatga taggttttaa tataggtaag tatatatatc
    60541 tactagatgc ctatgaagat ttagatgaag attttaagaa aggaagatat aatccattta
    60601 tagagtatat tgataaaaat gatgagttaa aagaaaaagt aaaaaaaata atagtaacat
    60661 cattaggatt tttagctagg ggaattgata atctaaatct aaataaaaat gttggaataa
    60721 tagaaaatat aatttattct ggtgtttatt taagatatat taatatatta gaaagtagag
    60781 gtggaaagaa tgtacagtaa tacttataat aatgaaaatt ttatgagagc aagatcattc
    60841 atagaaaata gtgaatttaa aaaagcatat gattttttaa aaactttgac tgacaaatgt
    60901 gcagagtggt attatttaac tggtttttct gcaatgaaca taggatatta tgaagaaggt
    60961 gaagacttct taaaaagagc aaaatttatg gagcctgaaa atagtgaata tagtgatgca
    61021 cttagaagtt atacacaata tagaaatgat tatagcaata gggctgacaa ttataataga
    61081 agaagacgta atgatttaga tggatgttgt tgctgttgct gtgatgattg ctgttgttgt
    61141 ctaggtgacg attgttgtga aaattgtgct aaactttggt gtttagattc atgttgtgaa
    61201 tgttttggtg gagatttaat aacttgttgt taggatgata aatatgagta taaaagttgc
    61261 ttatggagga gttttattag cattaaatgt gatattgttg actcttacaa acattattcc
    61321 tgtaaataca ttgtttatta tggggttagc atcactttta gtatcaattg taataatgga
    61381 atggggcttt aagagtggaa ttgcttttta tataggttct atagttttag gatttatagt
    61441 gatggcaagt aagtctcaat ggattgtata ttcacttacc tttggaatat atggtattgt
    61501 aaaatatctt atagaaaaag ataggtctat atatattgag tattttatga aattagtatt
    61561 tgcaaatata atgatactta tattgtattt tttattaaga acaatagttt atattccaat
    61621 aaacatattt attataggtt catttgagat agcctttata gtatatgact atgtttacag
    61681 ttcttttata ggttattaca ataatagatt aagaaagatg ctatttaaaa aataatagtt
    61741 gatttttata tttaatttta atgaaattgt agtttagata agtgtaaatg atatatatga
    61801 atatacatta aagcataaag ctaaatttaa tatattctta gtgcaacata taaaagcttt
    61861 gatttttagt caaagctttt atatgttaga tataaatatt agatataata taagttttta
    61921 aaatagatag tataaattaa atatccacta tatttacatc tgtagataga gaataaataa
    61981 tatcttttct ttctactatt atctcaacat ttttagaaat ttcctcatca agtgatattt
    62041 gagataaaag ttcctttgtt ggatttattg ctattggatt tccaactctt tttagcatat
    62101 ttatatcacc attagtatct ccataggcat acgagtttga taaatctaaa tcatacttta
    62161 caacaaaatc gtttatagca ttattcttac ttatagaatc ccacattgga ataactgtgc
    62221 ctgtaaatat attgttttca aagacataat cacttcctag aaaatcggtt acattatatt
    62281 tttttgccat tttttcaaca agaaatccag ggctaccaga tataaatata actatgtgtc
    62341 cttgatttag atgccactca atccttgaac gtgtgtattt ataaactcta tctgctttta
    62401 atttaattac ttggtcactt gtaaagttga tacaggtttt atctaatcct tttagtgaat
    62461 caacataaag gtcacatatt tctaatagat aatcatcata attaccttgt cttttatccc
    62521 aatccataaa agtgtctcta gcatgtttat accaattttt ttggtcaatt atatcgtatt
    62581 taattaattt tttaaaatgt tcaaccataa gagagtctct ataaagagta ccatctatat
    62641 caaaaaaagc tgctatattt ttcataagac cacctcaatt aatcatttcc ttatattata
    62701 ttataaattg tatctaagtt gttaaaatat atgtataata atttatatat taaaaaacaa
    62761 gtattttaga gtttaaagga tgtgactata tgaattttaa ttttgcaaag gctacaaatt
    62821 ctagacttat gggcagttta ggtcttataa taaattggat agatgatgaa aacaatcact
    62881 tttgtcaata ttttttatta gatgcagagg gattaggatt agcggattat gtgagtttaa
    62941 ataacccaac acaagaagaa gcttatatgg aagaagaaag attgatggga ggatttggtt
    63001 cagatagagt tgaattaacg aaggatgaat ctttattttt agtatcttat tttggtaata
    63061 agaatcttta ttatgataag ctattgccag gagataaatg tgagtatatt gatattataa
    63121 aaaactataa aacagattta actatagaaa aattatacaa taaaatatgt aaaagagttg
    63181 acgaggaagt tgaatttata aactatatga ccatgagatt tatagcatgg gatagagaaa
    63241 gtttaaagta cttttctgga agtgacgaga ttgcaaatat gcacattact aatataaatg
    63301 gaacattact taaaaacgtg gtaagtgata aaggtcaagg cagatatata agtgaagctt
    63361 tatatgaaga taatgatgga tattatatat gtaaaattgc tttttgcata agtaagtgtt
    63421 atgaaactgg ttttagaatt aattctctat tagtgacaga taaagaacct atgtatgatt
    63481 ttgaggtatt tgatgagatt tctaaaagtg aatttgtaag tgtttacagt gtaaattcac
    63541 ctgaagaatt tgcaggagta ttttatagag aaaatccatt tctgttaaaa agtaatatga
    63601 atgaaggtat attctttact agatttaatt ttaataatga ccatgtaaaa gaaaatgtat
    63661 atataataaa taatgatatg aaagctatat attacttaat gggaaataag ttttttattg
    63721 gtacttataa cgaaaatgat tgcaattata tcaatgaaat aagtcaatct aattatagcg
    63781 actatattaa atttgaagag aaatttttct ttgagcaaaa tgcattatat gattttgcag
    63841 aatcaggttc attagatttt gatgattttt tggaataata aatttagaca ttatttaagc
    63901 gagattaact gaatttaatc tcgcttaaat ttatttctat aatattgggg gaatattatg
    63961 aggaatacat ttattattgt ggtcaattat taaaatttta tacagtttat tataaaaaat
    64021 ttatattttt ctttatgttg tttgccaata ttagtctcaa atacgagtac catactattc
    64081 tgaatcacca acacaagaga gcatttttaa atgaaaaagt cagataaaat taaattttat
    64141 ctgacttttt ctatgtttat cttattagat tatgttaata tctgttgacc gaataagcgc
    64201 atattcagtc aactttctat acctataata atattggggg aatattatga ggaatacatt
    64261 tattattatg gtcaattatt aatgttttat acaacttact taaaaaaata tatattacat
    64321 ttcttcctca tcaaattcct caatattagt agcttgtggt ataccttgta cagacatatt
    64381 actagaacca taaggagtag gatatatatt aggaggcata gtactaggca taactacagg
    64441 cataccttgc attgtattat gacatataac tcctggtatc atcataagta cattaggagg
    64501 catattttga ttacatgaga agttaggaat acccatagta ttgttcatat tataccccat
    64561 attactgttc atgttattag gcatacctgt attaccatac atattgttat tcatattgcc
    64621 agacatattg ttaggcatgc ccatattact attcatatta ttagacatat tgttaggcat
    64681 acccatatta ctattcatgt tattaggcac attgttaggc atgcccatgt tactattcat
    64741 attattaggc atattgttag gcatacccat attactattc atgttattag gcatattgtt
    64801 aggcataccc atattactat tcatattgtt agacatatta ttaggcatac ccgtattatt
    64861 attcatgtta ttaggcatat tatttgtagc agatgtattg taattcatat ttccggatgt
    64921 attatttgtg ttaggcaagt tgtttggaga attactactt ttaggataat gatttacatt
    64981 attaggatat aggttattta tgttctggtt attaggtatt tttgtataac cattagaaat
    65041 ttgagggtca tttctcaatt catcttgggg aatttcttca ttagtaatat aatctgcata
    65101 gttcgctggg tctaagtaag gcatgtaact agtatctaaa tccttttttt tcttgttact
    65161 catattaaac ttgcacctcc tagaaaacta taaaaaagtt tacaaaaact tattctaata
    65221 atatatatat taaaaaagtt tataatatgt gcattctatt tatattaaat taataaaaat
    65281 tattgttgca aatttagaaa tgataacata taatgaacat atgaacagtt attcatatat
    65341 taaaataatg gaggattaaa aatgagagaa gagataaatg attgtaattg taatatagta
    65401 catgaagaaa tagtaacaga ggctaaatca actatgccag atgaagaaat gttatatgat
    65461 ttagcagaat tatttaaagt atttggggat actactagag taaagatatt atatgcatta
    65521 tttgcaaatg aaatgtgtgt atgtgatata gcgagtttat taaatatgac tcattctgca
    65581 atatctcatc aacttagagt attaaagcag gctagattag taaagtttag aagagaagga
    65641 aaaacagtgt attactcatt agatgatagt catataagtc agatatttga ttgtggatta
    65701 aatcatatta gagagactta taaatgataa atgagaggag tgaatatata tggaagcaaa
    65761 taattcaatt aaaaaagaat ttatactagg aggattaaat tgtgctcact gtgcagagga
    65821 gattaataat aaagtttcta aactacaaga agttaaatct tccaatttaa attttataaa
    65881 taaaaaactt acagtaaata taaaggaaag ttttaatgag gatactacta tagaaaagat
    65941 aatagatata atagattcaa cagatccagg tcttgatata caaataagtt ctaaagaaaa
    66001 tgcagctagt aaaacatcaa ttaaaaaaga gcttatatta ggaggattaa actgtgctca
    66061 ttgtgcagaa gagattaata acaaagtttc taaattaaaa gaagttgaat cttctaattt
    66121 aaactttgta aataaaaaac ttacagtaaa tataagtaat aactttgaag aggatgatgt
    66181 tataaataaa ataaaagaaa taataaattc aacagagcca gggcttgata tacaagtagg
    66241 gtctactgac aaagtaaaag ggagaactac tgaaaaatca ggagctgtaa atgatacaaa
    66301 taagaaagaa ttaattccac ttataattgg agcacttgta tatatatttg gtatatatca
    66361 aacagctaca ggttatgaaa gtcagtttag caatatagta tttatagttg cctatgtaat
    66421 agtaggtggt gatgtgcttt taagagcaat aagaaatata tcaaaaggaa gagtatttga
    66481 tgagaacttt ttgatggctc tagcaacagt aggagcatta acaatagggg agttatcaga
    66541 agcagtaggg gttatgcttt tctataaagt aggagagtat ttacaaggtg ttgcagttgg
    66601 taaatccaga aagtctataa cttcacttat gcaaataaga ccagactatg caaatctaaa
    66661 agttaattct gaagttaagg tggtgtcacc agaagaggta aatgtaggtg atataatagt
    66721 agtaaaacct ggtgaaaaag taccattaga tggtgtagta gtagatggag tttctatgtt
    66781 agatacttct gcactcacag gagagtcagt gctgagagaa gttgaaaaag gagatgaaat
    66841 cttatcaggt gttataaata aaaatgcttt attgagtatt gaagttacaa agagttttgg
    66901 agaatcaaca gtttctaaga ttcttgacct tgttgaaaat tctagcatta aaaaatcaaa
    66961 gacagaaaac tttatatcta agttttcaag atactatact ccaatagtag taatagctgc
    67021 tttattaata gcatttgtgc caccacttgt aatctcaggt gaggtattta gtgattggtt
    67081 atatagagga ttgatattct tagtcgtttc ttgcccttgt gcattagttt tatcaatacc
    67141 acttagtttt tttagtggaa ttgggtttgc atctaaaaat ggtatcctta ttaaaggaag
    67201 taactattta gaagctttaa gaagtgtaga tacagtagta tttgataaaa caggaacact
    67261 tactaaaggt gtgttcaatg taactaagtt aaatcctgaa ggtatatcag aagaagaatt
    67321 attagaatat gcagctattg cggaggtaaa ttcaaatcat ccaatagcaa agtccatatt
    67381 aagttactat aataaaaaaa ttgatttaga tacaatagac agttatgagg aaatagcagc
    67441 ttatggaatt agagtaaagc ataatggcaa ttttatatta gcaggtaatg agaaacttat
    67501 gaaaaaagaa aatatatctt attcatcagc taaagaagta ggcacagtag tctatattgc
    67561 tgttgataaa gtatatagag gttatatagt tatctctgat gaagtaaaag aagatagtaa
    67621 aaatgccata agaagcttaa aagaaatagg ggtaaaagaa gttgtaatgt taactggaga
    67681 taatgagaaa gtagcaaaaa atattgctca agaattagaa ttggatacag tttactcaaa
    67741 tttacttcct aatgaaaagg tagatagatt agaagattta tatgaaggaa gaactgaaaa
    67801 agagaaaata gcgtttgtag gtgatggaat aaatgatgct ccagtattag ctcgtgctga
    67861 tgttggtata gctatgggag gcttaggttc agatgctgct attgaagctg cagatgtagt
    67921 gcttatgaca gatgagccta gcaagatatc taaagcaatt gaaatagcaa acaagactaa
    67981 taagatagtg tggcaaaaca taatatttgc tctaggagta aaaattatag ttatgatact
    68041 aggtgctgga ggagtagcta ctatgtggga agctatattt gctgatgtag gtgtggcact
    68101 tatagcagta gtaaatgcta tgagagctat gagataaggg ataaatgtaa atccatacta
    68161 tttaaaaaac ttataattat atattgaagt atgtaataat gaaaggtatc aataagtttc
    68221 ttttaacttt attggtacct ttttattgga gcgctttgca tatataaatt tatattcatt
    68281 atccattatg tttatttaat gtatttattt agtgtagagc ttttatattg taattttaaa
    68341 atttagacat agaaggttta ttaataagtt tatattaagt ttaaattaga ttcataaaaa
    68401 ttataatact tatgattgta tatatattat attaatagtt gtaattttta caacaaaatg
    68461 tgtgtgttaa aatactctaa accaacagta tgactgccat tcaataaatt atgtaatata
    68521 agaaagtaaa tatttttaaa aaataatttt aaaaagtatt gacgaattat ttttaag
//
LOCUS       DAEPIN010000012        62816 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.012, whole genome shotgun sequence.
ACCESSION   DAEPIN010000012 DAEPIN010000000
VERSION     DAEPIN010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 62816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62816
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.012"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            complement(<1..988)
                     /locus_tag="KR250_000963"
     CDS             complement(<1..988)
                     /locus_tag="KR250_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HBH0066928.1"
                     /translation="MKAGEIEFLSYLEGSNKSFVVPVYQRNYNWKKEQCKRLFDDLED
                     IIDSGFRTHFLGSVVSIYSMGKEYLIIDGQQRVTTVSILLIAMCQIIKEFGESKGIIT
                     TEEQIKECYLINKHSRDKDGKIKLKPIAEDRKAYYELFEEEMNTDRNSNIVSNYLYFY
                     NRIKEDNISIDDLFDAIQKLTIVEIELKNGEDDPQLIFESLNSTGLDLSEADRVRNFV
                     LMGRSSKLQEEFYNNYWYKIEKNTSSNVSSFIRDYLTIKERSIPNKNKVYFSFKRYVS
                     DNGIDIEILLKDLLKFSNYYREISYSCTDDNDVNKVLKRINKLEIFVSYPFIL"
     gene            complement(1369..4704)
                     /locus_tag="KR250_000964"
     CDS             complement(1369..4704)
                     /locus_tag="KR250_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HBH0066929.1"
                     /translation="MGNFDFLQKKKNFNSFNTACLEAEKSILVSPSTCATITRRALEL
                     AVKWLYANDSDLVLPYQDNLSTLIHNNSFIELIDYDMLPLLKYIVKLGNLSVHTSANI
                     ERGEAILSLNNLHQFVSWIDYCYSDEYTAEDFNEDLLLHGEEKRTRPDELKNLYERLS
                     SKDKKLEEVIKENEELRKSLTEKRKVNTVNYDFKIDEVSEFETRKRYINIELKLAGWE
                     FGKDIWEEIEVQGMPNESGVGYVDYVLYGENGKPLAVVEAKRTSKDPKIGQQQAKLYA
                     DCLEKRYSQRPVIFLTNGFDMYIWDDYSDRKVYGFYKKSELQLMIDRRKSKKSLSSVT
                     INDEISNRYYQKEAIRAVCEALENKQRKTLLVCATGTGKTRIAISIVDVLSRHNWIKN
                     ILFLADRKALVKQAKKSFTKLLPNLALCNLLDSKDSPEDARMIFSTYPTMMNAIDDTK
                     SKDGKRLFTPGHFDLIIIDESHRSIYKKYKSIFDYFDSYLMGLTATPKDEIDKNTYSV
                     FDMENGVPTYAYEYNKAVEDGYLVDYTSIEFKTKIMEDGIKYDELSDEEKEEYENTFN
                     DDESIGDEIGNNAVNEWLFNSDTIDLVLNKLMTEGLKIEGEEKIGKTIIFAKNTKHAR
                     AIVERFNKLYPKYGGNFVKAVDYSINYVDSIIDDFSDKNKLPQIAVSVDMLDTGIDIP
                     EILNLVFFKKVRSKTKFWQMIGRGTRLCPDLLGVDMDKERFLIFDFCNNFEFFKFNPK
                     GFEGNKTETLTEKLFNIKVSMVKELQDIKYIEGEYAELRKELLEELITSVKALNEDSY
                     IVRMNLSYVHKYKNENVWSNIGAVAQNEIREYISPLITSYSDDELAKRFDVVMYNIQL
                     AYLQNNNASKGIRHVMATAEKLSKLGTIPQIQQQKYTIEKAMTEDFWEDSDIFEVEEV
                     RISLRELIKYLEKSSQKIYYTSFEDMIVAEDRNDSVYNANNLKNYKKKVEYYLNSHKD
                     ELAIFKLRNNKKITKQDVETLEEILLKQLGNCDDYKKEFGDTPVSQLVRRLVGLDREA
                     ANEAFSEFLNNKSFNTKQIHFVKLIVDYVVKNGFIEDNKVLMEDPFRTVGSIIDLFEN
                     HIEERNKLIKTINKIKENASEIG"
     gene            complement(4754..5182)
                     /locus_tag="KR250_000965"
     CDS             complement(4754..5182)
                     /locus_tag="KR250_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0066930.1"
                     /translation="MVIRNKLSWNSIEEVNRDFGSCLYDLQNFKILYDKEEMPELWAR
                     YIKEGFKSYMVSFLELTKAMLYYKSIDLNIKSKNFYDYLLACEYHNLLPKNSSIVIET
                     LRKLRNDDSHGYDIPQFEDMYELFTENEEVFVSIRNSCKK"
     gene            complement(5172..5552)
                     /locus_tag="KR250_000966"
     CDS             complement(5172..5552)
                     /locus_tag="KR250_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HBH0066931.1"
                     /translation="MRNIPNEIKNSIINVFPKENTLAIIIFGSYGTDKHTSESDIDIA
                     WIPIKKVPITELATKTEALRNILDIDVDLKIVTDNYTIALKKAILEGEVIYKSKDFKD
                     YINKFYFENSDVIDILDWRDMYGN"
     gene            complement(5585..5893)
                     /locus_tag="KR250_000967"
     CDS             complement(5585..5893)
                     /locus_tag="KR250_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0066932.1"
                     /translation="MNQIELDLYHNMVNIYKEADLQCNYKPTRFLQLINNKGALLAAK
                     ELINKEGATEGFTRLWECKRLDLSLEVLVLKEKYKELFTDEEREICINRLKDYGYKFN
                     "
     gene            complement(5938..7098)
                     /locus_tag="KR250_000968"
     CDS             complement(5938..7098)
                     /locus_tag="KR250_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HBH0066933.1"
                     /translation="MEYRLNEVFEFIRNGASIKQFDTKFGYPITRIETIANSYIDTNK
                     MGYADIKELGKLEDYLLKNGDILMSHINSEKHLGKSAIYEDINKKIIHGMNLLCLRPD
                     KEVCYPKYLNYFFNSNEFRRQLPRIIKKSVNQASFSVNDLKNLKVDLPEMEAQKDISK
                     VLDKAQELIDKRKEQIEALDELVKSRFIEMFGNFKEQKFVLLKDCTNFIDYRGKTPIL
                     SENGIRIINAKSVGNGVFKYINEYISENTYNSWMKRGFPVAGDVLFVTEGHTFGNVCR
                     IPKDLKKFAMGQRIITIQGDKKILNNAFLAQYMQTKAFRVEIDKYKTGSSAQGIRSKE
                     LQKILIPVPSIELQNKFEEFVNQVDKLKVGIKTSLKELEDNFNSLMQKAFKG"
     gene            complement(7102..8565)
                     /locus_tag="KR250_000969"
     CDS             complement(7102..8565)
                     /locus_tag="KR250_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HBH0066934.1"
                     /translation="MITGELRSKVDKIWETFWTGGLTNPLEVIEQFTYLLFIKGLDEV
                     ETKNEAEATLLGVSFEKIFDDEHQHLRWSKFSNEGNSEKMYEIVQNEVFPFIKKLHGN
                     KESAYAKYMGDAIFKIPTPLMLSKIVDGISNLELSKDRDTKGDLYEYLLSKVATAGTN
                     GQFRTPRHIIDMIVRLIKPTPEDIIVDPAAGSAGFLVSSQQYLRDNHSSLFLVQGLKE
                     HFNNGMFYGFDMDRTMLRIGAMNMMLHGVDNPNIEYKDSLSEVNTDKDKFTLVLANPP
                     FKGSLDYEAVSADLLKVTKTKKTELLFLALFLRVLKIGGRCACIVPDGVLFGSTGGHK
                     SIRKEIVDNHKLEAIISMPSGVFKPYAGVSTAIMIFTKTGTGGTDKVWFYDMKADGYS
                     LDDKRNEIKDSDIEDIIKRFDNLDGEVDRKRTEQSFFVDVEEIRENGYDLSINKYKEV
                     VYEEVVYDAPSVIIGRVRELEKEIVDGLEELERMLEV"
     gene            complement(9074..9943)
                     /gene="gatY"
                     /locus_tag="KR250_000970"
     CDS             complement(9074..9943)
                     /gene="gatY"
                     /locus_tag="KR250_000970"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HBH0066935.1"
                     /translation="MALVTTKQILLDAQEGHYAVGAFNVENMEMVMAVIEAAEELKSP
                     VILQTTPSTIKYAGLDYYLANVKVASEKASVPVAMHLDHGSSFGLAMQALRVGYTSIM
                     IDGSHESYEDNISISKSVVDACMPSDIPVEAELGKVGGKEDDLDGGDGGAYTDPLEAK
                     EFVERTGVSSLAVAIGTAHGLYKGEPKLDLDRLSEIREVVSVPLVLHGGSGIPDEVIK
                     ESIKRGICKVNYATELRIAYSNGVKDVLNSDPEVIDPKKYGKKGLESVKDFVKSRMEV
                     CGCVGKAIEIVKC"
     gene            complement(9963..11879)
                     /locus_tag="KR250_000971"
     CDS             complement(9963..11879)
                     /locus_tag="KR250_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIABC"
                     /protein_id="HBH0066936.1"
                     /translation="MGKKMILGVTGCPTGIAHTFMAEEALKNSARELGCDIKVETNGA
                     IGVENKLTAKDIEMADAIIVACDKNVDMDRFNGKPVIEVPVKEGIHKANELIQRCIDG
                     KVAIRKGNLSSSKIAEEGNLSFGQKLYKDLMNGVSHMLPLVVAGGVLTAISFLWGIYS
                     FDPNSEQYNQIAATLKSVGGYSMNLMVPVLAAFIAQSISGRAGMLAGLVGGMISFDTG
                     SGFLGGIISGFLAGYLVKLFVHLLRKLPRQLEGLKSIFIIPIVSVGLVGVAMLLLGGP
                     CSALNNAMMNFLSGLQNSSPIILGIVIGCMSAFDMGGPVNKAAYVTGTMLLGQGNYLF
                     MAGVSAACITPPLVIAIASTLFKNRFTEEDRAAGLINYILGSTHITEGAIPFAAKNPL
                     KVLPVLMIASSISSILTYIMKIEVPAPHGGFLVLGLVNKPLLWVGCIFVGSLVGAVLY
                     MMVTPKVVNEKNTIENTNVTKDEHKNESAESENVSNNISLYSEETVILDVKGKNKTDV
                     IDEMIGILDKSSVLLDKKKFKEEILKREEISSTGFGMGIAIPHAKTDAVKVPRVAVGL
                     SKEGFDFESEDGSLAHIIFMIAATDNGDNLHLKTLSQLSSKLMNEEFLNELLNSKTSR
                     EIVSKLNNEEIKSV"
     gene            complement(11882..12802)
                     /locus_tag="KR250_000972"
     CDS             complement(11882..12802)
                     /locus_tag="KR250_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HBH0066937.1"
                     /translation="MIYTLTTNPAIDMNISTNGIKRKLVNRTSNAVYSPNGKGLNVTF
                     VLGHYGIESKILGFFGGFSGKYIVEESEKRGFDVLPTWVEDTTRINIFLNDGSDEFKF
                     VNSGSYVNEKQKLDMLDKIQSLEDISYLSISGSLPPGIDDNYYEDIFKICENKSIKTI
                     LDISSPKLKELLQYNPYLIKPNDEEIKDIFGIIVRDEEDIKDVLKLLHLKGAQNILLT
                     LGEKGSYFYNGKSIYYASAQPVTVVSSACAGDSALAAFLSIWLENPEHIEEALKRSAA
                     TGASVAESNGIGNLENVEDYIKNIRVRKVV"
     gene            complement(12904..13656)
                     /locus_tag="KR250_000973"
     CDS             complement(12904..13656)
                     /locus_tag="KR250_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBH0066938.1"
                     /translation="MIIELNKSNSENLTKTELEIIKFINENESILPELSIVEIAHETY
                     SSPATVSRAIRKCGLNGFNELRYRLTVKDENDDIQNMGDIINKSFIEAQRVIEQISLT
                     TLINIIKTVNKSSKIYILARGLTEYVAKEFSLKLQLLDFNVVFINDPNIMKIKSKSIK
                     KDELIIIFSLNGNTIELVESAQNANLVGAKVITCCCSESAKLIKYSDLYIVGYKHEHI
                     SISEYEVSSRLPLYIISRIIIDYMVRYNNSEN"
     gene            complement(14048..15493)
                     /locus_tag="KR250_000974"
     CDS             complement(14048..15493)
                     /locus_tag="KR250_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBH0066939.1"
                     /translation="MIKLGKFPKGFLWGGAVAANQLEGAWNEGGKGWCLADVHMYDKD
                     RDISKPYESDMTIERIKFAMKDNNGYYPKRSGIDFYHTYKEDLKLLKELGLKCFRFSI
                     NWARIFPNGDDLEPNEEGLKFYDNLINEVIDNGMEPIVTLLHYETPLNLVLKYKGWSN
                     RKVVDFFVRYAELILNRYKNKVKYWIVINQINLIHYESFNSVAFCVDQVDNVEETRYQ
                     AIHHQFIATALTKKLGKEINSDMMIGMMMADCTSYPEDCDPENVVFALKRNRLQYFFT
                     DVSFRGEYPVYIKRFFKENNINIVMEEGDDKILKENTMDFLALSYYYSTTVSASKNTM
                     NPTDNIKNPYLKANPWGWAIDPKGLYNTLSQYYDRYQKPIMIAENGFGMHDKFEDGKV
                     HDSYRIDYLGKHIEQIGEAIADGTEILAYCMWSPIDIVSCSSQEMEKRYGFIYVDIDN
                     NGNGSKKRYKKDSFYWYQNVIRTNGEELKEK"
     gene            complement(15515..15868)
                     /locus_tag="KR250_000975"
     CDS             complement(15515..15868)
                     /locus_tag="KR250_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBH0066940.1"
                     /translation="MTIEEHIIDEELVEVAMQIILRAGEARTEIKHALNDLEKFDYRN
                     ADLKLAKAKGFMTEAHRAQTKIIQGEAGGEKRAHSLLFAHAQDTLMTIFSELNITMSL
                     VGIVKSIEYKVNNSK"
     gene            complement(15897..17144)
                     /locus_tag="KR250_000976"
     CDS             complement(15897..17144)
                     /locus_tag="KR250_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBH0066941.1"
                     /translation="MGRVFMNKFIDFLSEKFTPVVNNMTKNIWVQSVQSTIMKVLPMV
                     FVGSLVTIVSVLKNYISFLPDLSPINQYTFGLLGLFIAFLLPMEIMKNKKFESMSVVA
                     GLAGAGLMLMMIRPEITNEGAIFNFNRFGGEGMLVSLVAGLFSGLIMSLFGSFSFFGE
                     DSALPDFVSKWFDNMLPITVIMFVGWGLIYGLNFDMFEIIVRVFKPVSDIAQTLPGFV
                     LLNFLVVFIYSLGISGWVLSPITYSIQLGAIAANAAAVASGGVATNIYVYEVIQTGWI
                     TLGGLGATLPLVIMMCLSRVGRIKAIGRACIVPSIMNINEPVIFGAPVAWNPFLMLPM
                     WIGTVVISITTYLSMSMGFVTIPSKVFNLWYVPFGISTFTVNQDVRGLIVLAINIVLL
                     FMIWYPFFKAYEKEEIKLESQEV"
     gene            complement(17152..17469)
                     /locus_tag="KR250_000977"
     CDS             complement(17152..17469)
                     /locus_tag="KR250_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBH0066942.1"
                     /translation="MKNIRVLLACGSGASTGFMATSMRKSAKKKGIEADIKARSESEI
                     DKYLSEIDCLLLGPHLDYMKEEIQQKANEYNVPVACISKTAYGRLDGAKALEEALELI
                     SNK"
     gene            17711..19624
                     /locus_tag="KR250_000978"
     CDS             17711..19624
                     /locus_tag="KR250_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBH0066943.1"
                     /translation="MKTSKKELFLNFIKTQHDWIDSSTLANYLNVSTRSIRKYVNEIN
                     SNGEFILSSKKGYKVNLNNNCQTKVDSSENISPDNRLNLILKELIVNSNGINIFDLSE
                     DLFVSPATIEGDIVKANKFIGSYNLKIKQSKFLLKLIGNESAKRKLMSSIIFKETGSD
                     FLSLFDVQKIYQEYNLTKLKENIIYILKKYNLFINEYAINNILLHLMITIDRIKKNNY
                     IESVEVVNYIDNNNDISISRDISNFLESEYNITLTSAELYYLVFQLTNKTTVLNYHEM
                     DTKSLPNYIDEHFVKLTKKIIKNVYDLYFIDLSDDEFVVKFTLHVKNLISRAKNNQVL
                     RNQIPQKLKDSYPLIYDISVYICNQIQILENIEIDEDEISYISLHVGSFFDRQKLLED
                     KILCALITPNYYDLQFKIVRDLEKRFNESIEIIQIFSDTHNLDFDNKVDMVITTLPIS
                     DRCPIPFVYVNPYLNRKDYDNIQSKFIQIKDRKNILTVQNHLEMYFSESLFMKNIYLD
                     SAKDYIRFMGNILYENKYVKPNYIDDVLIREKMSSTAFNNNVAIPHSMKMDALKTGVC
                     LIVNDKPVKWGEEKVQIIAMIPINEKEKEKFNYIFESFIEILSEWNNVKELTKADNYS
                     SFMNRIAYLIQNI"
     gene            complement(19747..20901)
                     /locus_tag="KR250_000979"
     CDS             complement(19747..20901)
                     /locus_tag="KR250_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HBH0066944.1"
                     /translation="MKILISIDSLKGSLSSIEAGNAIKKGILKVKEDAQVKILPLADG
                     GEGTVDALVQGMNGKKDTIEVTGPIAKKVDATYGLLKNTSTAIIEIAQASGLTLVPTE
                     LRNPLYTTTYGVGEIIKEAINKGYRNFIVGIGGSATNDAGIGMLQALGFEFYDENNKL
                     VGLGGKVLNEIRHIKIENRLKELDECKFKIACDVNNPLFGKNGAAYIYGSQKGATSEI
                     IEELDNGLRNFSKVVKNYLSKDVANVEGAGAAGGLGFAFLAFLNSKLESGIKIILEEI
                     KLEEELKDADFVITGEGRLDNQTAMGKAPIGVAKLAKKYGVKVIGLAGATTEDAVKCN
                     EEGIDAYFSIVNRAMTIEEAMDKATASENMTATTTQIFNLITSIQQSKNM"
     gene            complement(21161..23776)
                     /gene="mngB"
                     /locus_tag="KR250_000980"
     CDS             complement(21161..23776)
                     /gene="mngB"
                     /locus_tag="KR250_000980"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HBH0066945.1"
                     /translation="MKKNVHIVPHMHWDREWYFSTEESRILLVNNMEEIMDMLENNHN
                     YPYYVMDGQTAILEDYLAVKPECKERIKKLVQEGRLIIGPWYTQTDEMVVGGESILRN
                     LLYGIKDCDEFGEYMKIGYLPDSFGQSAQMPQILNGFDIKYSMFWRGCSERKGTNKTE
                     FNWKSDDGSSVLVQILPLGYAIGKYLPMNEDELRTRMDKYLPVLDKGATTDHIILPNG
                     HDQMPIQKNIFEVIYKLKECYPERKFFLSRYENIFKELEKNEDIDTIKGEFLDGKYMR
                     VHRSIFSTRMDIKSANARIESKITNILEPLASIAYSLGFEYHHGLIELIWKEIMKNHA
                     HDSIGCCCSDKVHHEIMNRFFLAEEKVDQLITFYKRRIVDAMSCEMALDKLVAFNLMP
                     YDRNEIVSAQVITKMKAFEIFDKDNNKLDFEVVHKEVIDAGLIDRQIVHYGNYDSFMV
                     YTINFKDKIPAMGYKAYMIKEVEHMLEKAYEVCDMVDNDFYTIQVNENGTLKIFDKKL
                     NKTFENVLLMENGGDEGDEYDFSPLPNEKLIFNDNIVANSTIKKNRFNNEIKINYRLK
                     VPKDIQARKNNRADSFIDFDIVINVPNDKAIIDVVFNINNQARDCRVRTYIPTNIASK
                     FSVSDNQFGHIKRSVYDDAMEVWQQEEWSERPDSIYPMLTFVGLSDKEHGVAVLTNST
                     REFEIVGEKFDTIAITLFRSVGFLGKEEMARRPGRPSGIKLPTPDSQMIGDIKIDLAI
                     VTHEKSTLEANVANMAKEYLTKIQTYNKMPYNAMKLNQSEIEVDYSYSLLKETNKDLV
                     LSVVKKAEKQEALVVRFYNSKEETKSANIVFNKNIKTAKWINLNEKELESISIDNNSV
                     NVECLMNQVKTILVK"
     gene            complement(23920..25845)
                     /gene="mngA"
                     /locus_tag="KR250_000981"
     CDS             complement(23920..25845)
                     /gene="mngA"
                     /locus_tag="KR250_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HBH0066946.1"
                     /translation="MEFKKITNENLVFLKQKIKTKKDAIKFLARKLYDEGKLTSYEEY
                     LEAVMYRETLSPTGFERGLAIPHGKSKSVKEAAFAAMTLETPIENWESVDPNNKVEII
                     FLIAVPENEGGNTHIQILSELVTRLSNEDYMNMLLNSKTKKELFNNLDIKEKKEQIKE
                     EIRIQNESKKIILAITACPAGIAHTYMSAEALIKAGKEIGVDVYVEKQGANGMVDPHT
                     PDIIKRADAIIYATDVAPKNTERFEHLPNIKTSVAAPLKRAKEIIYEALEVAQKQGKG
                     DYIEKSSDISYCEKSSWKKDVKSAILTGVSHIIPLIVAGGMILAFAVLASQAFGLQEL
                     YNQEGSWLWQLRKLSGGMLGTLMVPVLSAYMSYSLAEKPGLGPGFAAGLAADLIGSGF
                     LGGMLGGLLAGYIVKFLKKNIPAKGTLAGFVSFWVYPVVGSLVVGLLMLFVVGKPVAA
                     LNNGLINWLNGMTGVNAILLGAIIGAMVSFDLGGPINKAAYTFAIGAIASGNFIPYAI
                     FASVKMVSAFGVTIATVLGRDLFSESEKEIGKQTWILGLAGITEGAIPFMIEDPIRVI
                     PSLVAGSAITGSIVAFFNIGLQVPGAGIFSLALLEGSMSGFIIASIWFFAAVLGAIIS
                     AVLLIITRKQKNQRKIH"
     gene            complement(25975..26700)
                     /locus_tag="KR250_000982"
     CDS             complement(25975..26700)
                     /locus_tag="KR250_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBH0066947.1"
                     /translation="MEFYERAKKYEYKFTDTEDMIIEYMRKNKEEIVKLSIQSLADKF
                     FTVPNTIVRLCKKMEYDGFSQLKNNLKEELKNEDKEPKNGFESDIKKTLELIDYEKIN
                     KVADIIQKSRSIIVYGIGQNIPLCEILVKEIRGGHDNITYYSQRHEVLHDIGKLGEQD
                     MFFALSLSGETKEVIEVAKTAVNKGCKVVALTHICENQLQQIADISLYCYSTIKQVNG
                     YNISDKVPIMMIMRAIIEQYWLR"
     gene            complement(26963..27733)
                     /locus_tag="KR250_000983"
     CDS             complement(26963..27733)
                     /locus_tag="KR250_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HBH0066948.1"
                     /translation="MKKLVVRADDIGYSEGVTLGIISSYKNGIVTSAGIMVNMPFSRQ
                     AIEMVKNDDVCFGLHVNLVIGEPCCKEHIKIKNLLDEGGKFVSSKIRRTQLLTGKEVF
                     NYEEVYEEVCAQIETYKLYMSKLPEYIDIHGIEEDVSISAMCDAAKEYGIPSCPYYLD
                     SCISMPSKYPQNDFYKLNKSFVKLFEEKYINLDKDITLMVTHPGFIDYSLIKSSSLIQ
                     ERVYEHAMLCDKEVKMWLKRNNIELVDFKDIFKSNNYI"
     gene            complement(27735..29162)
                     /locus_tag="KR250_000984"
     CDS             complement(27735..29162)
                     /locus_tag="KR250_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBH0066949.1"
                     /translation="MMQQLSLLGKTMLVPIAVMPAAGILGLLFGPNMLNVPAISNISN
                     IVFSNVDYLFLLGAASAYSKSKDKTSVMVAAVVAYMIFKSNLELLNDTLNAGVFGGII
                     VGACIAWTYNKTYKVKVPAFLSFFAGEKCVITLSPVVALILSYIFSLLWIYPQAAMNQ
                     FGFWLGGAGAIGIFLFLFCNRALIPTGLHQVLNAYILFEMGEYTTSTGEVIRGEIPRF
                     MAGDPNAGFFWTGFYIIMMFSIPAISYAIYKTAQDKKKQEVKGAMTAGALTSFMATVT
                     EPIEFSFLFASPKLYIIHSFYSGLGGVVLYLLGVRLGSFNGASIIDYVLSFSYGDKAW
                     LVIPVGVVFFVLYYVTFKYIIIKDNVQTPGREVEIEIGDKVSEKEKNLKLSHGNFSYM
                     AKQIIKNCGGYENIVTLNNCMTRLRLEVKDATILNDDNIKKTGAKGVIKLSNTSVQII
                     IGTDVVKVKDEMEMQLDELRKQADA"
     gene            29553..30275
                     /locus_tag="KR250_000985"
     CDS             29553..30275
                     /locus_tag="KR250_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBH0066950.1"
                     /translation="MAKNKFSEIYEVLKEEILDGKYTSNMMLPTELQLIERFSCSRNT
                     VRRAISQLNTEGYVQSIKGKGVVVLENSCSNDFFLNMHNFKGVESIVEDKKVNTATSV
                     LHFSKILIDNKLSKKTGFKVGSEVYYLHRLRYIDNIPKILDINYFLCSIVKDLDVSIA
                     QGSIYKYIEECLGTKIVSSRKIFKIEKATELELKTLPLNDYNCVGVIKNSVYTDDGKL
                     FEYTESKHTPETFVFMDVTQRY"
     gene            30472..32139
                     /locus_tag="KR250_000986"
     CDS             30472..32139
                     /locus_tag="KR250_000986"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HBH0066951.1"
                     /translation="MKNWWKKATVYQIYPKSFKDSNNDGIGDINGIIEKLDYLYYLGV
                     DLLWLTPMYVSPQRDNGYDIEDYYNIDPKYGTMSDFEKLLKEAHKRDIKIMMDMVLNH
                     TSTEHKWFKESKKSKDNPYRDYYFWKDAKPDGSVPNNWISRFSGTAWKYDETTNQYYL
                     HLFEETQADLNWENEKVREECYKILEFWADKGIDGFRLDVVNLLSKTPGLPDDPITGP
                     KGDGRTHYADGPRIHEYLHNMNQKVFKPKNIVTVGEMSSTTPEECINYTRENREELSM
                     VFSFHHMKTDYKGGAKWTNEMFSLDKLKKAQSDFQYKMYEGKGWNALFYSNHDQPRAL
                     SRFGDDRFFRQESAKMLAITLFGLQGTTYIYQGEEIGMTNAYFTKIDEYDDAESKNAY
                     YHMIKSGIDEKEALLILQQKSRDNARTPMQWDNTIYKGFSNHKPWLKVNNDNISVENE
                     LNDSRSVFYTYQRLIKYRKQYDIFTEGTYRLLDENHKNLFVYEREYKNQNLLVISNFT
                     HDNVVYKLPETYLNKLNYTILETNYTRDIIENKMEIKPYESIMIYYK"
     gene            complement(32235..33554)
                     /locus_tag="KR250_000987"
     CDS             complement(32235..33554)
                     /locus_tag="KR250_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBH0066952.1"
                     /translation="MEKGIKLGFWSIVLLGINAIIGSGIFGLPGDAYTDIGPASILVL
                     VFCMLLAVSIALCFAEAGSWFDTDGGPYLYAKEAFGDFVGFEVGFMKWIVSMIAWATM
                     ANFFAVTLSSVWPQAAEPLIKNIIIGILVVGLGIINFMGMKQSKHLNNIMTIGKLLPI
                     VLFIAVGLFFIKGSNFTPFVIIQKGQSASSAFVAVSITLFYAFTGFESLAVAAKDMEN
                     PKKNVPKALVMVMFVVSVIYMLILGISIGVLGNGLAGSATPVADAAIKMLGPIGGYII
                     TIGTIVSVGGINIASSIFTPRSAAALVEQGLMPKSIRQTNKNGAPYIAIIVSVIGTLL
                     IAWSGSFTTLSQISVVSRFIQYIPTCLAVLILRKKYAGKDVNFRIPGGAIIPIFAVII
                     SILLLIKAGIDEPMKIVWGLGGMIIVVPIYFYMTKVYSKKYNDVEVK"
     gene            complement(33595..34341)
                     /locus_tag="KR250_000988"
     CDS             complement(33595..34341)
                     /locus_tag="KR250_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HBH0066953.1"
                     /translation="MKKLAKKKYPIIGISGNLLIDEGGMFPGYERAYVNNDYIQSVVM
                     CKAIPYIVPIVYDDEIIKEQVSNIDALILSGGQDVNPLIWKEEPHNKLGAISPKRDSF
                     DMKLLKHALDMKKPVLGICRGEQIINVTEGGSLYQDLSLIEGAYIKHNQQHLSNVPTH
                     TVQIKEGTKLYEILGEKEVLVNSFHHLVVNKVAPGYIVSATSKDGLIEAIEKEGSEFV
                     IGIQWHPEMMTRDYDNMKKIFMAIVKEASK"
     gene            complement(34585..36594)
                     /locus_tag="KR250_000989"
     CDS             complement(34585..36594)
                     /locus_tag="KR250_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBH0066954.1"
                     /translation="MKTIAVVTDGLSKLECFLNKNLEMIFKDKVMIKNYYLNKITEKE
                     LIKDDIILVMIDDRVLKIKKYVEDVSKIITINRSIKQKDIYKLFSLPEGIDVLVVNDN
                     KHTVLETISNLYNIGINHLNFIPYNPNEEYRNIKIAITPGESSLVPKYIEEVIDLGHR
                     YIDSSTFIQIIAKLKLDDKEITERLIKYTDEIISLYSGINTKYKELTIKADELNSIIN
                     LSNLGMAMVSDKGNIIICNNSLRDILDIQSNIIGKNIDELENENIKKIFSLSKAHDEV
                     IKFNNKYLNVNKYNIESFGRVTGYYFCIQEITYIRKLEQNLSKKLREKGQVARYTFDD
                     IKTVSTKMERTKKLGMKIAESDYTILITGESGTGKELMAQSIHNASPRHSQPFIAINC
                     AAMPENLLESELFGYEEGAFTGALKGGKKGLFEQANNGTIFLDEIGDMPIYLQSKLLR
                     VLQENQVMRVGGENVIDIDVRVIAATNKNLLEMIKGDRFRADLYYRINVLPINIPPLR
                     ERKEDIEVMLKHFMKRKRAISQDVQDIINAYEWPGNIRELKNTAMYINIMSSEDVILI
                     NDLPHNLLNITEDFSKEISILKDRTSIEKVKLVMECLKNANNLNKNIGRNGIVNSINE
                     LGYNITEGEVRGILNMLKELDLIICESGRKGSELNRRGKNILNSI"
     gene            complement(36988..38421)
                     /locus_tag="KR250_000990"
     CDS             complement(36988..38421)
                     /locus_tag="KR250_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBH0066955.1"
                     /translation="MALRKDFLWGGATAANQCEGAWNLDGKGPSCSDMCTGGSQKVSK
                     RITRVIEKGTFYPSHEAIDFYHRYKEDIALFAEMGFKVFRFSIAWTRIFPTGMEKEPN
                     EAGLKFYENVIDECLKYNIEPLITISHYEIPFAITEKYNGWVSRDVIDLYMNYCETIF
                     KRYKGKVKYWLTFNEINSATMPMGGYLSQGILNEGTTDFINQVDIPQLRFQGLHHQFV
                     ASAKAVKLAHEIDSNYQVGCMQIFATMYPYTCNPDDAVKTQRDSRVMNYFCGDVQVRG
                     EYPTYMNRYFGENNIEIKMEKGDLEILKEGCVDFYTFSYYMSTCVSSDSKEDNTSGNI
                     LGGVKNPYLKSSEWGWQIDPEGLRYALNEIYDRYRIPMMVVENGLGAYDKKESNGVIN
                     DDYRIEYLKAHIEQMKEAVEDGVDLFGYTPWGCIDLVSASTGEMAKRYGFIYVDKYDD
                     GSGDLSRIKKKSFDWYKQVIESNGERL"
     gene            complement(38508..40424)
                     /locus_tag="KR250_000991"
     CDS             complement(38508..40424)
                     /locus_tag="KR250_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBH0066956.1"
                     /translation="MRKYEQLSKEIIANVGGKDNISSLSHCITRLRFKLKDESIANDD
                     VLKKMDGIVTVMKSAGQYQVVIGNHVPDVYEVVCDLAGLGGDTIDNSDSDAPKGLLNK
                     FIDIISGVFQPILGVLCAAGMIKGFNAVFTALGLYGETDGAYIMLNAIGDAMFQYMPI
                     ILGYTAAKKFKLKPFIGMLIGAVLCYSGIQLDTLASTGQPLYNLFTGTIFESPVYITF
                     LGLPVIAMNYTSTVVPVILIVLLAAKLEKVFAKIIPDVVKNFFVPMAVLLVSLPLGFM
                     VIGPIATYASNIVGNGFLALFNFSPILCGALTGLLWQVLVIFGLHWGLVPIAMSNLMT
                     MGFDTILVGVFVPSFAQTAVVAAMYFKLKDKKIKELCIPAVISGICGVTEPAIYGITL
                     PRKTPFIYSCVGGAVAGAVMGIMNVKSYVMGGLGIFGIPNYINPTTGDMKGVYATIIA
                     IIVAIVVGFVLTLFFWKDDAIVEISKDDNGQGVIEKNKREVVYSPIKGEVKALEQIQD
                     AAFSTGILGKGVAITPKEGKVVAPFDGTVMTLFPTKHAIGIISDNGCELLIHIGLNTI
                     QLEGKYFKSFVNQGDKIKKGQTLITFDIESISKEGYCLETPVVVTNYSDYVDIVENTQ
                     KHVNNSQKLITVLA"
     gene            complement(40498..41358)
                     /locus_tag="KR250_000992"
     CDS             complement(40498..41358)
                     /locus_tag="KR250_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBH0066957.1"
                     /translation="MIIEKILNNNVIITTDENHKEIVVMGRGLAYKKRTGEHISKDKI
                     DKIFKLSDPNISDKFKELIADIPIRYMELSDEIILYAKEKLGKRLNDSIYISLTDHMY
                     TAIERAREGVSVKNVLLWDIKRFYKSEFKIGLEALDYIEKKFEIRLSEDEAGFIALHI
                     VNAQMDQSIKTIYEITQIIQEISNMVKYHYRIVFDEDSVYYHRFITHLKYFAERIVSN
                     NLHENNEDDLLNVIKVKYKNAYKFIEKLDEFIHKKYNYDLTDEEKLYLTIHVERVVSK
                     SIKSDINPNN"
     gene            complement(41709..42896)
                     /locus_tag="KR250_000993"
     CDS             complement(41709..42896)
                     /locus_tag="KR250_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBH0066958.1"
                     /translation="MIESIKKRAYEIENELGKEIEEVCNFIFNNPELGEEEYISSKYL
                     VEKMKEYGFDTVYPYCNMETAFRAELGDNDGPTIAFLAEYDALPGYGENKEPAHACGH
                     NWIAASTLGACIVLSKLKENFKGKIVLIGTPAEETTGGKCDLVKAGAFDDVDVSYQMH
                     IEAFNNINCKALAIDSLEFSFEGVSAHAASHPHMGVNALDAVQLTFAGINALRQHVKS
                     DVRIHGIVSNGGEAPNIVPEKAACKFFVRAAERSYLDEVTKKVINCAKGAELMTGAKL
                     SYRYFENSFDNIINNKVLQKITKNNLIEAGITDILEGKDGPVGSTDIGNVSQVCPTMY
                     TEIALDISPMVYVHEKEFLNYANSEEAYDKLHKAVKAMVGCALEIYLEDGLLDEIKKN
                     HLN"
     gene            complement(42910..44301)
                     /locus_tag="KR250_000994"
     CDS             complement(42910..44301)
                     /locus_tag="KR250_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HBH0066959.1"
                     /translation="MSSLKKKKFTMPQTLTIIFLLIVLMAILTWIVPSGNFERVDIDG
                     RSVVVAGTYEKAPSNPQGITDVFTAPINGFIDAAEVVGFVLIVGGAFGIVNKTGAIEA
                     VIAHTVNKMKKFQFLIIPISMILFGLGGTTFGMSEETLPFYMIFIPLMTSMGYDSLTA
                     VATVFIGATAGFGAATTNPFSVGIAQALSQIAPGSGIEFRVVMFIIYMAISIGFVMMY
                     ANKVKKDPKKSLVHDISLNQESMVNSDTNIKEFTKREAMVIAIFTIGMAIMIYGVLKL
                     GWYITEIAMIFTAIGIISGIASGLKQDDIVNSFISGAGDLMTAGLCIAFARGIVIIAE
                     NGFIIDTILNSAANLLNGLPKTIFINLTFLIEGLIAFLIPSASGLASLTIPVLAPLGD
                     LVDVSRQMIVTAYQFGIGVTNLITPTSGVLMGALAVANIPWSKWVRFVIPLMVVLTIL
                     VMAFLTIGLYLGF"
     gene            complement(44896..46596)
                     /locus_tag="KR250_000995"
     CDS             complement(44896..46596)
                     /locus_tag="KR250_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBH0066960.1"
                     /translation="MHYIAWHFNKNGGNNTMKDKLQVLSGAMMIPVILLVVAGVFIGL
                     GSAFANMENVSALGLSGLIKEGSFINVFFKVINDLGFMVMRYLPIFFVTGIAFGLSKK
                     EKGWGALGGIVLFIGMHTVISTLLAANGINSDTVTAEAFMASGLGEAEAYSRSSLYGS
                     FLGIFSYDCSIFGAIIAGFVASAVHNKFVDIELPSALSFFSGPRFSMIMMFIASIVLG
                     TAMYFIWPPVGLALSKLGNWIGSSGLLGTFTFGAMDKALLPFGIHHLIAFPIEYTRVG
                     GTMEVGGAIYEGVNNIRLAQMGDPDTLSYITRNFTTGRLLIHFAILPAAALAMYKLAD
                     NANKKKAISILIPAIVTAMLVGVTEPIEYTFLFVAPLLYFVAYVPLAGLTYVFTEMFN
                     VSIMGESFRNMFPNLLQPQKVDALPLLFLIPIFFIVTYIIFTWAIKKFDIKTPGRSSE
                     EVKLYSKKEYRERQSVDAGKTAYNNAVEEEVDDKDTKLVHSIIEGLGGSNNIKNVTNC
                     ATRLRVELNSIDGFYEDGFWVNELGASGVVKKKNSVQVIFGPRVITIASKLKAVLGVD
                     "
     gene            complement(46568..47971)
                     /locus_tag="KR250_000996"
     CDS             complement(46568..47971)
                     /locus_tag="KR250_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBH0066961.1"
                     /translation="MIVNKELIIDYLKSNQENIINDIRSLVEIPSIRDESTTDINQPF
                     GIEIRNAFDKLIQIAKDKDFVVKDFDGYAIHIEYGEGEEVVGVLNHIDVVPIYNKELW
                     KSKPFKVCQKDNYLYGRGVNDNKGPLIGILYALLFLRELNEKPKRKIRLIVGGAEETT
                     WECMEHYFSVNEQPKFAFSPDGNFPIVNGEKGILYFNLRKKIDKDKFRNHNLVDIKSN
                     KEDGFVCDKIEAVFKTNDKKDLVESLAYYTEIEELEEGKVLVRYTGERALSRNPHRSY
                     NCAFSLAKDLEKIKKLNDKGVIIRDILNSYFTDDNHGKKLGIYKEDVDMGVSTICIMS
                     IFLEKNELNMKIDFRYPKGISWEFITNRINEIGKKENLIVDIYKDLKLLYVKPDSELI
                     NKLSNAYKQGFGKEAELFTKGAASYARVLKNGVAFGPTIEGDNPNSHQANENISIDTL
                     YKAIEVYIYALYSLAFQ"
     gene            complement(48235..48726)
                     /locus_tag="KR250_000997"
     CDS             complement(48235..48726)
                     /locus_tag="KR250_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBH0066962.1"
                     /translation="MFNIFKKKENKIEKIYSPFKGNLVNLEDVPDAVFAQKMMGDGVA
                     IIPSEGKLYSPVDGEIVNVFDTKHAIMIKSNDNTNILIHVGLETMSLEGKPFDVKVKA
                     GDKVKAKDLIMEVDLDFITSNDLNIITPVVIMDDADSVKRTVKDRIEPGNVDINSIIM
                     EVE"
     gene            complement(48752..49564)
                     /locus_tag="KR250_000998"
     CDS             complement(48752..49564)
                     /locus_tag="KR250_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBH0066963.1"
                     /translation="MRYKLICTDMDGTLMGKGFEVSEENIKALKEVMEKGIKVALVTG
                     RPYNAMKYFTSVLGDDIYIISTNGTYFKLLGYEYKKVLSKEALKKIYTIGEKYNLSKH
                     FKGCKIVISNNEIGEEHPYRLINSKNKEEDRIEIIENASCETLLEKADNEILKCILFS
                     ENVDSLREAKEEFKKQEDLEVVSSGKINFEVMSKGTSKGIAVKKFCDILGIDSKEVIC
                     IGDNENDISMIKFAGLGIAMGNATDEVKSMADFVTDTNVNDGVAKALRKILS"
     gene            complement(49577..50728)
                     /locus_tag="KR250_000999"
     CDS             complement(49577..50728)
                     /locus_tag="KR250_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HBH0066964.1"
                     /translation="MINFYQPSRINFGQGTLSELPRIIGKYGKKCLLVTTPNVEPLDK
                     LYKRVTLNLIEKGIEVVHFDRVEPNPTVELINEGFELAKKESVDVVLAVGGGSSIDTA
                     KVLALTFGLEKIDWDYMFSTYTNPFKIYDKVSEKELPLIAVSTTSGTGSQVTQAAVIS
                     RGSEKNTIFHQNCFSKECIVDPELMLTLPERITASTAFDAFTHAFESYINPNANDFTE
                     IMSEKSMELVINYLPKVMEDKNNIEYRTKLAVADTLGGSSLANAGAAAPHPLSEIIGG
                     VTHIPHGEALAIVFPKFAEKFYKDNIEKFAKVARMLNQELEKVEDDEAAKTLGNEIES
                     FLKRVGLKTKMSEFNVTKEQLEQITSCPVLGFLPFGKKEDLVEILEKSY"
     gene            50998..51639
                     /locus_tag="KR250_001000"
     CDS             50998..51639
                     /locus_tag="KR250_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HBH0066965.1"
                     /translation="MKYLIDSANLDEIQKCLKYGFKGITANPSMYLKEKVNFHEFIET
                     CANLNPEILTAEVIGNNLYELINSCDKILNINKNTIIKINFSEVGLELINILNSKGVK
                     TACTLIFNVNQASLAINAGADYLFPFIGRNDENGNDGIKNLTEICNLISINGYNTKVV
                     AASIKNVFHLTQASLCGCDYAAVTYDLFKKASFNQLTIDGANTFERDWSKVNK"
     gene            51665..53209
                     /locus_tag="KR250_001001"
     CDS             51665..53209
                     /locus_tag="KR250_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBH0066966.1"
                     /translation="MNKRLLDIAELLLNSSDYITVDTIAEELKVSNKTIRNDLVILDE
                     WLLEFNLSLDKKTGSGVIILGNEDIKLKVIRDINDKSNCIYAYSPEDRKRYILSKLFI
                     KNSKFRIRDICNELHVSRATVHKDLVVIQNFLQNFKVTLVRKTNNGVYLEGKEKDIRK
                     AIFELATVNKSYTELKEILFSKSNECKDTPSTKIFKELFNYDFEKLSQITLHTLQVEK
                     SNLSDEYYINFLIHIAICIKRISIKRYINLSEGFFRELLEHEYFEKSKILCANLSEAF
                     DVEFIDEEVCYILLHIEGLLKSIKKTTTLEPLKNEEITEYNLSEAIAVHWGSILNLNL
                     KDDLILIKSLANHLKSVLHRINYGFTITNPILNDIKRTFPYTYKAAKESNTVIKQLMN
                     YEVNDDEIGYLALYLISAIDRSKSPLNTILICHCSDGVSNLLVQKLSFEFNQINIVKS
                     IPLSSISFTNFDDVDLILTTAPVDFEHNAELININALLSKNDISRLSTIIKKLYSEKN
                     KILSYK"
     gene            complement(53409..54035)
                     /locus_tag="KR250_001002"
     CDS             complement(53409..54035)
                     /locus_tag="KR250_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBH0066967.1"
                     /translation="MKKEMIKDYLLITISAFLMALAVNFFFAEHTLAPGGITGLSVVL
                     STFLSMPVENISLGISMPLLVMGLLFLGKGFGIKTLYITLMGPLFLKVIPQTHITDNL
                     LVAGVIGGLLVGTAIGIAIIRGCSTGGTDLVAMLINKVIKFLKLPVILFMLDGFIIVA
                     SGIISKNFMISIYSLISLLLIIKTIGFVTTKFGKNTEEINNINNACAQ"
     gene            complement(54170..54886)
                     /locus_tag="KR250_001003"
     CDS             complement(54170..54886)
                     /locus_tag="KR250_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentose-5-phosphate 3-epimerase"
                     /protein_id="HBH0066968.1"
                     /translation="MKHIKIAAGLAHVNYGHISAVVKEAMDAGVDYVHSDAADMHDLK
                     NMQLMGGHQIIEAIRPVTDKPIECHIYTRDCDRLFIEKIAAAGCNLLIIPAEHFLGAP
                     LAYIINYCREFGMKVGLTIGCYTPLCFVDEAIYDIDRLQIVVHGVDETDGKDNWGWRK
                     SAVDLVKRARKMIDEKNPKCELAIDGGLRADNMEPLIECNPDVVILSSAIFKDKDGIT
                     AGVKNCRKAIDKAATKFGLE"
     gene            55312..56526
                     /locus_tag="KR250_001004"
     CDS             55312..56526
                     /locus_tag="KR250_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HBH0066969.1"
                     /translation="MRNCIIAQSGGPTAVINASAVGIYEKNLETKYFDKVYFGINGIE
                     GILNKNIIDSDSLNKDNVLNLKQTPSSGLGSCRYKLADYKINSYDYDRVINILDEYEI
                     DTLFYIGGNDSMDTVMKFSQYCKNTTCKIKFIGIPKTIDNDLLGTDNCPGFGSAAKLI
                     STLIMETYLDFSIYKNNGIFIVETMGRDTGWLAASSSLARLNDTPLVDLIYLPEIDFY
                     EDKFIEDVRKIFKEKNKAYIVASEGIRDKYGNFICEQKTSAHDKFGHSQLGGVSNYLK
                     NLIVSSSITTRVKTLELGVIQRCAMHLASDTDLKQAAEVGAYGIELSKKGYTGVMAII
                     NRTCNNPYKYEVSHTDISKIANKTKYFPKEWINEECNYITDEALNYLYPLIQGKPDIK
                     YKNGLPYFSIIY"
     gene            57146..58474
                     /locus_tag="KR250_001005"
     CDS             57146..58474
                     /locus_tag="KR250_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0066970.1"
                     /translation="MMKIAVITTEFLKEFVDNSIKKLNINAEIEIYIYRDFSHVGDLY
                     LEIEDRFDGFAVSGPIPKKAITKKAGTIKKPLVDFGTDLQSYYDMFLKLIFKYNTLDF
                     SRAYFDLTDWADNPKDISYYLSNGTFGDLMDSIDENASKMSLSDIAKLEKKIAKKHIN
                     LWNEGKIDFSTTRFSSIVPMLQNAGVNFHFIYPDIDLLKNTFNNLIKDIHLHRMKGNQ
                     PSVIYITVKDTNYDDSTLDQVYKILNSIKKDKLTDFIIEKNSNSFKIFTKCETIKSLT
                     KNFTACYIKESIENSTNIKVCVGYGIGNDIKQAMSSAISANKESKINHLNYSFLVNEN
                     YETIGPLLCDKLLTVSNKVTPHINTIAKSSKLSTLTIQKIISVTEILQRDEVTPKELS
                     SCLNVTVRTANRFLSSLLKSGNATILYEKQNSTKGRPERVYKLFISENKY"
     gene            complement(58602..59792)
                     /locus_tag="KR250_001006"
     CDS             complement(58602..59792)
                     /locus_tag="KR250_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBH0066971.1"
                     /translation="MKNFFKEAQSIEGEIIRWRRYLHENPEIGCNLPKTVTFVTSTLE
                     KMGYSPKLTSNNGIVAVLDSKNGGKTLLLRADMDALPIMEETDLPFKSKNKDASHSCG
                     HDNHTAMLLGAAKILMDNKHLLKGRVKFVFEPDEETTRGAKSMIDDGILENPQVDAAM
                     AFHSMVGKFLNTGQVAYSRGPAMASADIFEITVEGKATHGASPEFGVDPINIMSHIQI
                     ALNTILSREKPQKEPVVLTIGMFNAGSVPNAIPNKASMSGTLRTFDQSVREKLKNRII
                     EISTGIAKSFGGNAKVEFKMGTPAVINDLDVGDEIVSYVKEVIGNENVIDFPSAMGSE
                     DFSEILLRVPGVFLWIGMGSISQGYNFGMHNPKAIFNEEGLVYGSALFAHCAVKWLEN
                     NFSR"
     gene            complement(59898..61277)
                     /locus_tag="KR250_001007"
     CDS             complement(59898..61277)
                     /locus_tag="KR250_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBH0066972.1"
                     /translation="MIGSISINEIDKKRFKLDDLSKKIWENPEKAFKEFKACENTANF
                     LRSEGFDVEVGVGGLATAIRASFGSGKPVIGFMGEFDALPGLNQKVSTKQEAFELGAY
                     GHGCGHNLLCTAHVGAVVGLKREMIENNLSGTIVFYACPGEEQLTGKGFMARGGAFEG
                     LDLAINFHPNKISEATIGTSTAVNSVKFHFKGKTAHAGSDPQNGRSALDAVELTNVGA
                     NYLREHVTSDVRIHYTITDGGVAPNIVPDKASVWYYTRALSREAVESTYERLVKVAKG
                     AAMMTETEVEVEFLGGCYNTLNNHVLANLVSECMNEIKQEPWTREELDFAAELDRQSP
                     QQYKAMISKYNLPEGTHLYYGGGNVTNFNSYGSTDIGDVMHIVPTAYFFTGCTNLGAP
                     GHSWQFTSCAGSSIGEKGMIYASKIMAMFGAKILNNPEIAKKAKEEFDKSMNGKTYKC
                     PIPDDIPTP"
     gene            complement(61308..62516)
                     /locus_tag="KR250_001008"
     CDS             complement(61308..62516)
                     /locus_tag="KR250_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0066973.1"
                     /translation="MENFWNPLTCFTVCVMIYAFSEFLSKKTKGAISTLLFVCIIFLL
                     GFWTNILPKDITGASGILAAMSNFGIALLITNLGTLINLEDLCHEWKTVVIALVGIVG
                     IGVGCLTVGSMLFGREYALIAAPPIAGSTVAGIIVTTVAEAANRPELAAFVILVLSFQ
                     KFFGIPITTYCINKELKFKRDNGDFLKDESKSNFKLPSMRIFKEDLGKKSSTLVYLAK
                     LSFVAMIGNFVGLATLIPGFTPANYYLNPNIACLLVGLIFTRIGFLEKGILDKSQSNG
                     IIMFGCMLMLPGGLAKVTPTALFGMIIPTIGILVVGSVFIILFTAIVGKLLGYSPRMS
                     AAIGVTCMLAYPATQIISNEGIDAMEGTEEEKQRATDYLLPKMIIGGFVTVTIASVAF
                     ASIIGPIIFG"
BASE COUNT        20487 a        11249 c         6917 g        24163 t
ORIGIN      
        1 ctaatataaa cggatatgat acaaatattt caagtttgtt tatccttttc aaaactttgt
       61 ttacatcatt atcatcagta catgaataag atatctctct ataatagtta gaaaatttaa
      121 gtaaatcttt taataaaatt tctatatcta taccattatc tgatacatac cttttaaaac
      181 taaaatatac tttattttta ttcggtatac ttctctcctt tatagtcaaa tagtctctta
      241 taaaactact tacgttgcta cttgtatttt tttcaatttt ataccaataa ttattataaa
      301 attcctcttg taacttagag cttctaccca ttaacacaaa gtttcttact ctatcagctt
      361 cacttaaatc taagccagtt gaatttagac tttcaaagat taattgaggg tcatcttcac
      421 cattttttaa ctctatttct actatagtta atttctgtat agcatcaaac aaatcatcta
      481 ttgatatatt atcttctttt attctattat aaaaatataa ataatttgaa actatattag
      541 aatttctatc tgtattcatt tcttcttcaa atagttcata gtacgctttt ctatcttctg
      601 ctattggttt taactttatt tttccatctt tatctcttga gtgcttgttt ataagataac
      661 actccttaat ctgttcttct gttgtaataa ttccttttga ttccccaaac tcttttatta
      721 tttgacacat ggcaatcaat aatattgaca cagtagtcac tctttgttgt ccatctatta
      781 ttaaatattc ttttcccata ctatatatag atacaactga ccctaagaaa tgagttctaa
      841 atccagaatc tattatatcc tctaaatcat caaacaatct tttacattgt tctttcttcc
      901 agttataatt tctttgatat acaggtacta caaaactttt gtttgacccc tctaaataac
      961 taagaaattc aatttctcct gctttcatat taactccccc tataagttaa ataaattcca
     1021 ttcataactc catgtatcta tatattatat caaaattttg actcataaat gtatatgaaa
     1081 gccttattat ataccttttt atacctgttt ttattttatt aaagtaaata catgattatt
     1141 ctataaaata tacaaatact taattttaaa aattattatt taatcttctt catgagtaat
     1201 tcatagttat ttatcaatta aaaagagata ttgcataatt attcaatacc tctctttgta
     1261 tatttacatt atccatatct agttatctat ttcaacccaa tattagacta cctactaata
     1321 aatttattct ctacatcatt aacaataatt ttgcactaaa taacatcatt agcctatctc
     1381 tgatgcattt tctttaattt tatttatagt tttaatcaat ttatttctct cttcaatatg
     1441 attttcaaat aaatcaatta tacttccaac agttctaaat gggtcttcca tcaacacttt
     1501 gttatcctct ataaatccat tcttgactac ataatcgact ataagtttaa caaaatgtat
     1561 ttgcttagta ttaaagctct tattatttaa aaactctgaa aaagcttcat ttgcagcttc
     1621 tctatccaat cccactagtc ttctcactaa ttggcttact ggagtatctc caaactcctt
     1681 cttataatca tcacaattac ctaactgttt taagagtatt tcctccaacg tttcaacatc
     1741 ttgctttgtt atctttttat tatttcttaa tttaaatatt gctaattcat ctttatggct
     1801 atttaaataa tactctacct tctttttata attcttaaga ttgtttgcat tatatacaga
     1861 atcatttcta tcttctgcta caatcatatc ttcaaaactt gtataatata tcttttgact
     1921 agacttttca agatacttaa taagctctct taatgatata cgtacttctt ctacctcaaa
     1981 tatatctgaa tcttcccaaa aatcctctgt cattgctttt tcaatagtat atttttgttg
     2041 ctgaatctgt ggtatagtcc caagttttga aagtttttct gctgtagcca ttacatgcct
     2101 tataccttta cttgcattat tattttgtaa gtatgcaagt tgtatattat acataactac
     2161 atcaaatctt ttagccaatt catcatcact atatgaagtt atgagtggtg atatatattc
     2221 tcttatctca ttttgtgcta ctgctccaat attgctccaa acattttcat ttttatactt
     2281 atgtacataa cttaaattca tcctaactat ataactatcc tcatttaaag cttttacaga
     2341 agttataagt tcttctaaca attcttttct aagttctgca tactcacctt ctatatattt
     2401 aatatcttga agttctttaa ccatacttac ctttatatta aataattttt cagtaagtgt
     2461 ttctgtttta tttccctcaa atcctttagg attaaattta aaaaattcaa aattattaca
     2521 aaaatcaaat attaagaatc tttccttatc catatcaaca cctaataaat ctggacataa
     2581 tctagttcct ctacctatca tttgccaaaa cttagtctta cttctcactt tcttgaaaaa
     2641 aacaagattt agtatttcag gtatgtcaat tcctgtatca agcatatcta ctgaaactgc
     2701 tatttgaggt aatttattct tatcagaaaa atcatctatt atagaatcta cataatttat
     2761 actatagtca actgctttta caaagttccc accatacttt ggatacagtt tattaaatct
     2821 ttcaactatt gccctagcat gctttgtatt ctttgcaaaa attatagtct taccaatctt
     2881 ttcttctcct tctattttca acccctctgt cattagctta tttaaaacta aatctattgt
     2941 gtcagaatta aatagccact catttactgc attattccct atttcgtctc caatactctc
     3001 atcatcatta aatgtatttt catattcttc cttctcttca tcagaaagct catcatactt
     3061 aattccatct tccataatct tagtcttaaa ctcaatacta gtataatcta ctaaatatcc
     3121 atcctcgact gccttattat actcataagc atatgtaggt actccatttt ccatgtcaaa
     3181 aactgaataa gtatttttat ctatctcatc ctttggtgta gcagtaagac ccattaagta
     3241 agaatcaaaa taatcaaaga ttgacttata ctttttataa atacttctat gcgattcatc
     3301 tattataatt aaatcaaaat gaccaggtgt aaacaatctc tttccatcct tagacttcgt
     3361 atcatcaatt gcattcatca ttgttggata tgtagaaaat atcatcctag catcttctgg
     3421 gctgtctttg ctatccaata aattacataa cgccaagtta ggtaatagct tagtaaaact
     3481 ttttttagct tgctttacca atgcttttct atctgctaga aataatatat ttttaatcca
     3541 attatgtctt gaaagtacat caactataga tatagcaatt cttgtttttc ctgtaccagt
     3601 agcacatacc aaaagagttt tgcgttgctt attttcaaga gcctcacata cagccctaat
     3661 agcttctttt tgataatatc tattggatat ttcatcattt attgttacag aacttaaaga
     3721 cttcttactc tttcttctat ctatcataag ttgtaattct gattttttat aaaatccata
     3781 aactttccta tctgaatagt catcccaaat atacatatca aaaccattag tcaaaaatat
     3841 taccggtctt tgactatatc tcttttccaa acaatctgca tataatttag cttgttgttg
     3901 accaattttc gggtctttac tcgttctttt cgcctcaact actgctaatg gcttaccatt
     3961 ttcaccataa agaacataat ctacatatcc cacaccactt tcattaggca taccttgtac
     4021 ttctatttct tcccatatat cttttccaaa ttcccaacct gcaagcttta attctatatt
     4081 tatatatctt tttcttgtct caaattcgct tacttcgtct atcttgaaat catagtttac
     4141 agtattaact tttctctttt cagttagtga ctttcttaat tcctcatttt cttttattac
     4201 ttcttcaagc tttttatctt ttgaacttaa tctctcataa agatttttaa gttcgtctgg
     4261 tctcgtcctt ttctcttccc catgaagtaa taaatcttca ttaaagtctt ctgctgtata
     4321 ttcatctgaa taacaatagt ctatccaact aacaaattga tgcaaattgt taagtgaaag
     4381 tattgcttct cctctctcta tgttcgcact agtatgtaca gataaattac ctaatttaac
     4441 tatatatttt aataaaggta acatatcata gtctataagt tctataaaac tattattatg
     4501 aatcaacgta ctaagattat cttgataagg caatactaaa tcactatcat tagcatatag
     4561 ccattttact gcaagctcta aagctcttct tgttatagtt gcacaagtag atgggctaac
     4621 tagtatactt ttttctgctt ctaagcaagc agtattaaaa ctattgaaat ttttcttctt
     4681 ttgtaaaaaa tcaaaattac ccaaggcttt tatccccctt ttgtccctta gttaaaataa
     4741 atctctctaa aaactatttc ttacagctgt ttcttatact aacaaatact tcttcatttt
     4801 ctgtaaacaa ttcgtacata tcttcaaact gtggtatatc atatccatga gaatcatcat
     4861 ttctcaattt tctaagtgtt tctataacta tagaagaatt tttaggtaat aaattgtgat
     4921 actcacaagc taaaaggtag tcataaaagt ttttagattt tatgtttaaa tcaattgatt
     4981 tataatacaa cattgctttt gttaattcta gaaatgatac catatagctt ttaaagcctt
     5041 cttttatgta tctagcccat aactcaggca tttcttcctt atcatataag attttgaagt
     5101 tttgcaggtc atacaagcaa gaaccaaaat ccctatttac ttcttctatt gagttccaag
     5161 ataatttgtt tctaattacc atacatgtcc ctccaatcta agatatctat tacatcactg
     5221 ttttcaaaat agaatttatt tatataatcc ttaaaatctt tagatttata aataacttca
     5281 ccttctaaaa tagctttttt aagtgctatt gtataattat ctgtaacaat ttttaggtct
     5341 acatcaatgt ctaatatatt tcttagagct tctgtcttag ttgcaagttc tgtaattggt
     5401 acttttttga tgggaatcca agctatgtct atatcacttt ctgatgtatg tttatctgtt
     5461 ccataactac caaatattat gattgctaga gtattttctt ttggaaatac atttattatt
     5521 gagtttttaa tctcatttgg tatatttctc atttaatcac ctcttgttta ttaggtaata
     5581 ttgcttaatt gaatttatat ccataatctt ttaatctatt tatacatatt tctctttctt
     5641 catcagtaaa caactcttta tacttttctt ttaatactaa tacttctaat gataaatcta
     5701 atctcttaca ctcccatagc cttgtaaatc cttcagtagc tccttcttta ttgattaatt
     5761 ctttagcagc taataaggct cctttgttat ttatcagttg taaaaatctt gttggcttat
     5821 agttacattg taaatcagct tctttataga tatttaccat gttatggtat aaatctagct
     5881 ctatttgatt catagatttt attcccccat tttaataatt gtattttagt tgaataacta
     5941 tcctttaaaa gctttttgca ttaatgagtt aaagttgtct tctagttctt ttagacttgt
     6001 ttttattcct actttcaatt tgtctacttg attaacaaac tcttcaaact tattttgaag
     6061 ttctattgat ggtactggta ttaatatttt ttgaagttct ttgcttctaa ttccttgtgc
     6121 agagctacca gttttatatt tatctatttc taccctaaaa gcttttgttt gcatatattg
     6181 tgctaaaaaa gcattattta aaattttttt atctccctga attgttataa tcctttgacc
     6241 catagcaaac ttttttaaat cctttggtat cctacataca tttccaaatg tatgaccttc
     6301 tgtaacaaaa agtacatctc cagctactgg aaaacctctt ttcatccaag aattataggt
     6361 gttttcagat atatattcat taatatattt aaatacacca ttccctacag attttgcatt
     6421 tattattcta attccattct ctgaaagaat tggagtttta ccacgataat caataaaatt
     6481 tgtacaatct ttcaataata caaacttttg ttccttaaaa tttccaaaca tttcaataaa
     6541 tcttgattta actaattcat ccaatgcttc tatttgctct tttcttttat ctattaactc
     6601 ttgagcttta tctaaaactt ttgatatatc tttttgagct tccatttcag gtaaatctac
     6661 ttttaaattt tttaaatcat taactgaaaa acttgcttga ttaactgatt tttttattat
     6721 ccttggtaac tgtcttctaa attcattact attaaaaaaa taatttaaat actttggata
     6781 acaaacttct ttatctggtc gtaaacataa aagattcatt ccatgtataa tttttttatt
     6841 tatatcttca tatattgcac ttttccctaa atgtttttca ctattaatat gtgacattag
     6901 tatatctcca tttttcaata aatagtcttc taattttcct aactctttta tgtcagcata
     6961 tcccatttta ttagtatcaa tataagagtt tgctatagtt tctattcttg taattggata
     7021 tccaaatttt gtatcaaatt gtttaatact agccccattt ctaataaatt caaaaacttc
     7081 attcaatcta tattccatac tctacacctc aagcattctt tctaattcct ctaatccatc
     7141 tacgatttcc ttctctaact ccctaactct acctataata acactaggag catcataaac
     7201 tacttcctca tacactacct ctttatactt attaatagat aaatcatacc cattctctct
     7261 tatctcttcc acatcaacaa aaaagctttg ctctgttctt tttctatcta cttcaccatc
     7321 aagattatca aatctcttaa taatatcctc tatatcacta tcctttatct catttctctt
     7381 atcatccaaa gaataaccat cagctttcat atcatagaac cataccttat cagttccacc
     7441 tgttcctgtt ttagtaaata tcattatagc tgtagatact cctgcataag gcttaaacac
     7501 tccacttggc attgatatta tagcttccaa tttatgatta tcaactattt cttttcttat
     7561 cgatttatgt ccacctgttg aaccaaacaa tactccatct ggcactatac atgcgcatct
     7621 tccaccaatt tttaaaactc ttaaaaaaag tgccaagaat aataactctg ttttctttgt
     7681 cttagtcact tttaataaat ctgctgacac tgcttcataa tctaaacttc ccttaaaagg
     7741 aggatttgct aacaccaatg taaacttatc tttatctgta ttaacctccg ataaagaatc
     7801 cttatactct atattaggat tgtcaacgcc atgcaacatc atattcatag cacctattct
     7861 aagcatagtt ctatccatat caaatccata gaacatacca ttattaaaat gctccttcag
     7921 cccttgcact aagaaaagac ttgaatgatt atcccttaag tattgttgtg atgatactaa
     7981 aaaacctgcc gaacctgctg ccgggtctac tataatatct tctggtgttg gtttaataag
     8041 tcttactatc atatctatta tgtgtcttgg agtcctaaat tgaccattag tacctgctgt
     8101 agccactttg gataataaat actcatacaa atctcccttt gtatctctat ccttacttaa
     8161 ttctaaatta ctaataccat caactatttt agataacata agaggcgtag gaattttaaa
     8221 tattgcatct cccatgtact ttgcataagc tgattcttta tttccatgta atttttttat
     8281 gaaagggaac acttcatttt gtactatttc atacattttt tctgaatttc cttcattaga
     8341 aaacttactc caccttaaat gttgatgttc atcatcaaat atcttttcaa aagaaacacc
     8401 caaaagagtt gcttctgctt cattttttgt ttctacttca tccaatcctt taataaataa
     8461 aagatatgta aattgttcta taacttctaa tggattagtt aatccaccag tccaaaaagt
     8521 ctcccaaatc ttgtccactt tactccttaa ttctcctgta atcatatatg actttccctc
     8581 cagtgttcat aaaataattt aaatatatat tttagtcgta cgccttaata gttagtgtct
     8641 tatgttaaat ccaagttttt actacacaat tattaaatta tttttcctat ttacatattt
     8701 tagctaaaaa actaatgcaa tactcttatt ttatgattat tatatatacc atacaaaatt
     8761 attttatcat atatccatca tagaagtcat ctacctggat aactcttacc atattataac
     8821 tttttaatat taatttaaac ttagaaagaa attttttatt taattatatc atcttcctta
     8881 tttcaaattt ctcaattaca atcttaatca aaaaataaaa taagatgctg tctcaaaata
     8941 gatataatct tcttttttat tgtcggaatg atgaaaatat atttaatttc atacaaaaaa
     9001 gagtgcctca tgaactaaaa gttcattttg agacacccct taatttaatc ttacttattc
     9061 tttcccttta ttattaacac ttcactatct ctattgcttt tcctacacat ccacaaactt
     9121 ccatcctact cttaacaaaa tctttaactg actctaaacc ttttttacca tattttttag
     9181 ggtcaattac ctctgggtca gaattaagca catccttaac cccattgcta taagcaatcc
     9241 ttaattcagt tgcataatta accttgcaaa tccctctttt tatagactcc ttaataacct
     9301 catctggtat tccagaccca ccatgtaata ctaatggaac tgatacaacc tctctaattt
     9361 cagataatct atctaaatca agttttggct ctcccttata tagcccatgt gccgtaccta
     9421 ttgctactgc taaagatgat acccctgttc tctctacaaa ctcttttgct tctaatgggt
     9481 ctgtatatgc tccaccatca ccaccatcta aatcgtcttc cttacctcca actttaccta
     9541 attcagcttc aacaggtata tctgaaggca tacatgcatc tacaacagat ttagatattg
     9601 aaatattatc ttcataactt tcatgagaac catcaatcat aatagaagta tatccaactc
     9661 taagtgcctg catagctagt ccaaagcttg aaccatggtc taagtgcatt gctactggaa
     9721 cacttgcctt ctctgatgct acctttacat tcgctaaata ataatctagt cctgcatact
     9781 ttattgttga aggtgttgtt tgcaatatta ctggtgattt aagttcttca gcagcctcta
     9841 ttactgccat aaccatctcc atattttcaa cattaaatgc acctactgca taatgccctt
     9901 cttgtgcatc taataatatt tgtttagttg tcactaatgc cataatttct cctcctatat
     9961 gtttatactg atttaatttc ttcgttattt aattttgata ctatttctct acttgttttg
    10021 ctatttaata actcatttaa aaattcttca ttcattaatt ttgaagatag ttgagataat
    10081 gtttttaagt gtaaattatc accattatct gtagctgcta tcataaatat aatatgtgcc
    10141 aagcttccat cttctgattc aaagtcaaac ccttcttttg atagacctac tgctacacgt
    10201 ggtaccttta ctgcatctgt ttttgcatgt ggtatcgcta tccccatacc aaaacctgta
    10261 gatgatattt cttctctttt aaggatttct tctttaaact tctttttatc taaaagaaca
    10321 ctagatttat caagtattcc aatcatttca tcaattacat ctgttttatt ttttccctta
    10381 acatctaaaa ttactgtttc ttcgctatat aaagagatat tattagatac gttttccgat
    10441 tctgcacttt catttttatg ttcatctttt gttacatttg tattttctat agtatttttc
    10501 tcatttacta ctttaggtgt aaccatcata taaagtacag cacctactaa tgaacctaca
    10561 aatatacacc ctacccataa aagtggctta ttgactaatc ctagaaccaa aaaccctcca
    10621 tgtggagctg gtacttctat tttcattata taagttaaaa ttgatgatat tgatgatgca
    10681 atcataagta ctggcaacac ttttaatgga ttttttgctg caaaaggtat tgccccctct
    10741 gttatatgag tactacctag tatataattg attaatcctg ctgctctatc ttcttctgta
    10801 aatctatttt taaataaagt agatgctatt gctattacca atggtggtgt tatacaagct
    10861 gcagatacac ctgccataaa taaataattt ccctgtccta gaagcatagt tccagttaca
    10921 tatgctgctt tatttactgg acctcccata tcaaatgctg acatacatcc tattactatt
    10981 cccaaaatta tagggcttga attttgtaac cccgatagaa agttcatcat agcattgtta
    11041 agtgctgaac atggcccacc aagtaacaac attgctacac ccacaagacc tacgctgaca
    11101 ataggaatta taaaaatcga tttaagacct tctaattgtc gtggtaattt tcttaacaaa
    11161 tgtacaaata gtttaactaa gtaacctgct aaaaatcctg atatgattcc acctaaaaac
    11221 cccgaacctg tatcaaatga tatcattcct ccaacaagtc ctgccaacat accagctctt
    11281 cccgatatag attgagctat aaatgctgcc aatacaggaa ccatcaagtt catagaatat
    11341 ccaccaacag attttaatgt agctgctatt tgattatatt gttctgaatt tgggtcaaat
    11401 gaataaatcc cccataaaaa tgatatagca gtaagtactc cacctgctac tactaaaggt
    11461 aacatgtgag atacaccatt cattaagtct ttatacaact tttgaccaaa cgataaattt
    11521 ccttcttctg caattttact gcttgataag tttccttttc taatagctac ttttccatca
    11581 atacatcttt gtataagttc attagcttta tgtatacctt ctttgacagg tacttctatt
    11641 actggctttc cattgaatct gtccatatca acatttttat cacaagcaac tattattgca
    11701 tctgccattt cgatatcttt tgctgttaac ttattttcta cacctatagc tccattcgtt
    11761 tcaactttta tgtcacaccc taattccctg gctgaatttt ttaatgcttc ttctgccata
    11821 aaagtatgag ctattccagt tggacaacct gttaccccta gaatcatttt ctttcccatt
    11881 tttataccac ctttctgact cttatatttt ttatataatc ttccacattc tccaaattac
    11941 ctataccatt actttcagcc acacttgctc ctgttgctgc tgaccttttt aatgcttctt
    12001 caatatgttc tggattttcc aaccatatac ttaaaaaagc agctaatgca gaatcaccag
    12061 cacatgcaga gctgactact gtaactggtt gagcactagc ataataaatt gattttccat
    12121 tataaaaata agaacctttt tctcctaaag ttagcaatat attttgagca ccttttaaat
    12181 gtagtaattt aagtacatcc ttaatatctt cttcatctct tacaataatt ccaaatatat
    12241 ctttgatttc ttcatcattt ggttttatca aatatggatt atactgtagt aactccttta
    12301 attttggtga acttatatcg agtattgtct ttatactttt attttcacaa attttaaata
    12361 tatcctcata gtaattatca tcaataccag gaggtagact tccacttatt gataagtaac
    12421 ttatatcttc taatgactgt atcttatcta gcatatctag tttttgtttt tcgtttacat
    12481 atgacccaga gtttacaaat ttaaattcat cacttccatc atttaaaaat atatttatac
    12541 gagttgtatc ttctacccaa gtaggcaata cgtcaaatcc tctcttttcg ctttcttcaa
    12601 ctatatattt tcctgaaaat cctccaaaaa atcctaatat cttagattct ataccataat
    12661 gacctagtac aaaagttaca tttaatccct ttccattagg agaatataca gcatttgatg
    12721 tacgatttac taattttctc tttatcccat tagtagatat attcatatct attgcaggat
    12781 ttgtagttaa tgtatatatc attgtcttcc cccttgcttg tgctgtgtat gttttcatta
    12841 taataaaaaa gtctatgaaa atcttttcac aaatagaaaa tcgattttca tagacttttc
    12901 aatttaattt tcagaattat tatatctgac catataatct ataattatcc tagatataat
    12961 atacaagggc aatctggaag atacttcata ttcagaaatt gatatatgtt catgtttgta
    13021 tcctacaata tataaatccg aatactttat aagttttgca ctttcactac aacagcaagt
    13081 tataacctta gctccaacta aattggcatt ctgtgctgat tctacaagct ctattgtatt
    13141 tccattcaat gaaaatataa ttataagttc gtcttttttt attgatttag attttatttt
    13201 cattatgttg gggtcattta taaaaacaac attaaaatca agtaattgca atttaagact
    13261 aaactctttt gctacatatt cagtaagccc tcttgctaaa atatatatct tactagattt
    13321 atttacagtt tttattatat tgattagtgt agttaaagat atttgttcta taactctctg
    13381 tgcttctata aaggatttat ttattatatc ccccatattt tgtatatcat cattttcatc
    13441 ttttactgtt aatctatacc taagttcatt gaatccattt aaaccacatt ttctaattgc
    13501 tcttgaaacg gttgctggag atgaatatgt ttcatgagca atctctacta tagataattc
    13561 tggtaatatt gattcatttt catttataaa tttaattatt tcaagttccg tcttggttag
    13621 attttcagaa ttacttttgt ttagctcaat tatcatatgt tatctcccct tatataagtt
    13681 ttatttgtct aaatttatcg agtaatataa tatattacct cttaactact tatttttata
    13741 tacaattaag atttagtcaa tgtctatata tgtaaattta taatataata attttttgct
    13801 taaagtttat tatgttatct tatagcattt taaaataaaa aagaattgta cctctaccaa
    13861 aatttggatg aagtgcaata gatattcctt atactgatat gtttatgatt aaaagtataa
    13921 atataaattt cgctatgaat catataatta ataaatcatt ttatttacaa ttataacccc
    13981 ccttataaaa ctatattaat aatatataca gtatttataa ggggggttgt ttatatttaa
    14041 aaactattta cttttctttt aattcttctc cattagttct tattacattt tgataccaat
    14101 aaaaactatc ttttttataa cgttttttac ttccatttcc attgttatct atatctacat
    14161 atataaatcc atatcttttt tccatttctt gagatgaaca acttactata tctattggtg
    14221 accacataca ataagctaat atttcggttc catcagctat agcttcacct atttgttcta
    14281 tatgttttcc taaataatct attctgtaac tatcatgaac ttttccatct tcaaatttat
    14341 catgcattcc aaatccattt tcagctatca ttattggctt ttggtatcta tcataatatt
    14401 gagataaagt attgtataat ccttttgggt caattgccca cccccaagga tttgctttta
    14461 aatatggatt ctttatattg tcagttggat tcatggtgtt tttacttgct gatactgttg
    14521 tagaataata gtatgataaa gctagaaaat ccatagtatt ttcttttaag attttatcat
    14581 caccttcctc cataactata tttatgttat tttctttgaa aaatctctta atatagactg
    14641 gatactctcc cctaaaactt acatcagtga aaaaatattg tagcctattt ctttttaaag
    14701 caaatactac attttcaggg tcacaatctt ctggataaga tgtacaatct gccatcatca
    14761 tacctatcat catatctgaa tttatttctt ttccaagctt cttagtcaaa gctgtagcta
    14821 tgaattgatg atgaatagct tgatatctag tctcttctac attatctact tggtctacac
    14881 aaaatgcaac tgaattaaat gattcataat gtattaaatt tatttgattt ataactatcc
    14941 aatatttaac tttattttta tatctattta gtattaattc tgcatatcta acaaagaaat
    15001 ctaccacttt tctattagac catcctttat attttaatac taaattcaaa ggagtctcat
    15061 agtgaagtag tgttacaata ggttccatac cattatctat tacttcattt ataagattat
    15121 cataaaactt taatccctct tcattaggct ctaaatcatc tccatttgga aatattctag
    15181 cccaatttat tgaaaatcta aaacacttta accctagctc tttcaaaagc tttaaatctt
    15241 ctttgtatgt atgataaaag tctattccac tacgcttagg ataatatcca ttattatctt
    15301 tcatagcaaa ctttattctt tctatagtca tatcagactc ataaggttta gatatatctc
    15361 tatctttatc atacatatgt acatcagcta aacaccaacc tttgccacct tcattccatg
    15421 caccttctaa ttgattagct gctacagctc ctccccataa aaaaccttta ggaaacttac
    15481 ccaatttaat cactccttat tttatttatt taaatcattt actattattt accttatatt
    15541 ctatagactt tactattcct actaaagaca tagttatatt taactcgctg aatatggtca
    15601 taagagtatc ttgagcatgt gcaaataaca gcgaatgtgc tcttttctct ccaccagctt
    15661 ccccttgtat aatcttggtt tgagctctat gtgcttctgt cataaaccct tttgctttag
    15721 ctagttttaa atcagcattt ctataatcaa acttttccaa atcattaaga gcatgtttaa
    15781 tttctgttct agcctcacct gcacgaagta ttatttgcat tgccacctct actaattctt
    15841 catctattat atgttcttca attgtcatat ttataatttc ctttcaattt ctaaatttaa
    15901 acctcttggc tctctaattt tatctcttct ttctcataag ctttaaaaaa tggataccat
    15961 atcataaata gcaatactat atttattgct aaaacaataa gacctctaac atcttgattt
    16021 accgtaaaag tagatatacc aaaaggaaca taccataaat taaacacctt acttggaatt
    16081 gtcacaaatc ccatactcat tgataagtaa gtagttattg atattactac tgttcctatc
    16141 cacataggta acattaaaaa tgggttccat gccactggag ctccaaatat tactggttca
    16201 tttatattca ttatagaagg gacaatacat gctcttccta ttgcttttat tcttccaact
    16261 ctggataaac acatcattat aactaatggt aatgtagcac ccaaccctcc taaagttatc
    16321 caacctgttt gaataacttc gtaaacatat atattagttg ctactcctcc tgatgctact
    16381 gctgctgcat tagctgcaat tgcaccaagt tgaattgaat aagttatagg agatagtacc
    16441 cacccactta ttcctaaaga atatataaat actactaaaa agtttaagag tacaaatcca
    16501 ggtaatgtct gtgctatatc tgatactggt ttaaatactc taactattat ttcaaacatg
    16561 tcaaagttga gtccataaat taatccccag cctacaaaca ttatcactgt tataggtaac
    16621 atattatcaa accacttgct tacaaaatcc ggaagtgctg aatcttctcc gaaaaatgaa
    16681 aaagaaccaa ataaggacat tattaatcca gagaataatc ctgcaactag cgatacaagc
    16741 attccttctc ctccaaacct gttgaagtta aatatagctc cttcattcgt aatctctgga
    16801 cgaatcatca ttaacattaa tccagcacct gcaagaccag cgactacact catactttca
    16861 aatttcttat tcttcattat ttccataggc aataaaaatg ctataaataa tcctaaaagt
    16921 ccaaatgtat attgatttat tggacttaaa tcaggtaaaa agcttatgta attcttcaag
    16981 acagaaacta tagttactaa tgagcctaca aataccattg gcaaaacttt cattatagtt
    17041 gattgaactg attgtaccca tatattctta gtcatattat ttacaacagg agtaaatttt
    17101 tcacttaaaa aatctataaa tttattcata aacactctcc ccataaatat tttatttgtt
    17161 tgatattagt tctagtgctt cttccaaagc ctttgcccca tctagtcttc catatgcagt
    17221 tttagatata catgctactg gaacgttata ttcattagcc ttttgttgaa tttcttcctt
    17281 catataatct aaatgtggtc ctagtaataa acaatctatt tctgataaat acttatctat
    17341 ctctgactca cttctcgctt ttatatctgc ttctatacct tttttctttg cactctttct
    17401 catactagtt gccataaaac ctgtacttgc tcctgaacca cacgccaaaa gcactcttat
    17461 atttttcata atgatacctc caatattttt atccatcaat ttatttctta atgaactttt
    17521 tattttatta tgtttatatt gtacttacca aataagttca tttcacataa atatatttcc
    17581 taataatagg aaatcagtta ctcttaatta aacatattaa tatagtataa tgtatcttat
    17641 aatacgtaca ttcaattata taattatcat atcatccaga ttttatttta aaactatgcg
    17701 agggaatcta atgaaaacat ctaaaaaaga actattttta aatttcataa aaacacaaca
    17761 tgattggata gattcatcta cattggctaa ttatcttaat gtctcaacgc gttcaattag
    17821 aaaatatgta aacgaaatta attccaatgg tgaatttata ttatcctcta aaaaaggata
    17881 taaagtaaat ctcaataata attgtcaaac caaggttgat agttcagaaa atatatcacc
    17941 tgataacagg ttaaatttaa ttttaaaaga acttatagtt aatagtaatg gaattaatat
    18001 atttgattta agtgaagact tgtttgttag tccagctact attgaaggag atatagtcaa
    18061 agctaataaa tttataggaa gttataattt aaagataaag caatcgaaat ttttattgaa
    18121 actaattgga aatgagtctg caaagcgcaa attaatgagc tctataatat ttaaagaaac
    18181 gggaagtgat tttttaagct tatttgatgt acaaaaaata tatcaagagt ataatttgac
    18241 caaactgaaa gaaaatataa tatatatatt aaaaaaatac aatttgttta taaatgagta
    18301 tgctataaat aacatacttc ttcatttaat gataactata gacagaataa aaaagaataa
    18361 ctacatagaa tctgtcgaag tagtaaacta catagataat aataatgata taagcatttc
    18421 aagagatata tcaaattttt tagagtctga atacaatata actttaacta gtgcagaatt
    18481 atactactta gtatttcagt taacaaacaa aaccacagtc ttaaattatc atgaaatgga
    18541 tactaagtca ttgccaaatt atatagatga acattttgtt aaattaacta aaaaaataat
    18601 taaaaatgta tatgacttat actttataga tttatctgat gatgaatttg ttgtaaaatt
    18661 tactcttcat gtaaaaaatt taatcagtag ggctaaaaat aatcaagttt tgagaaatca
    18721 aatacctcag aagttaaaag attcctaccc tctgatatat gatatatctg tttatatttg
    18781 taatcagatt caaattttag aaaacataga aatagatgaa gatgaaatat cgtatatatc
    18841 tcttcatgta ggttcatttt ttgatagaca aaaattacta gaagataaaa ttctctgtgc
    18901 attaattact ccaaattact atgatttaca attcaaaata gtaagagacc ttgagaagag
    18961 atttaatgaa tccatagaaa ttattcagat tttttcagat actcataatc ttgattttga
    19021 caataaggta gacatggtta ttactacttt accaattagc gatagatgcc ctataccttt
    19081 tgtttatgta aatccttacc taaatagaaa ggattatgat aatattcaaa gtaaatttat
    19141 tcagattaaa gatagaaaaa atatacttac tgtacaaaat catttagaaa tgtactttag
    19201 tgaatctttg tttatgaaaa atatatattt agatagtgct aaagattata taagatttat
    19261 gggaaatatt ctgtacgaaa ataaatatgt caaacccaat tatatagatg atgttcttat
    19321 aagagaaaaa atgtcttcaa ctgcatttaa taacaatgta gcaattcctc atagcatgaa
    19381 aatggatgct ttaaaaaccg gagtatgttt aattgttaac gataaaccag ttaaatgggg
    19441 tgaggaaaaa gttcaaatta tagctatgat acctataaat gaaaaagaaa aagaaaagtt
    19501 caattacata tttgaaagct ttattgaaat attatctgag tggaataatg ttaaagaact
    19561 tactaaggca gataactata gtagttttat gaatagaata gcttatctta ttcaaaatat
    19621 ctaaatcaat taatttattt ccaatatatg tttcttaagt tattaacata aaatagataa
    19681 gctgtcacac taaaaagtct aaattaatat gtgacagctt tttacctaat ataccttgtt
    19741 ttaatactac atattttttg actgttgtat actagttata agattgaata tttgtgttgt
    19801 tgtagctgtc atattctcac tagcagttgc tttatccatt gcttcttcta ttgtcatagc
    19861 tctatttact atagaaaaat aagcatctat accctcttca ttacatttaa ctgcatcttc
    19921 tgttgtggct ccagccaaac ctattacttt taccccatat ttttttgcca gttttgcaac
    19981 tcctattgga gccttgccca tagcagtttg attgtccaat ctaccttcac cagttatgac
    20041 aaaatctgca tcttttaact cttcctctaa tttaatttct tctaatatta tttttattcc
    20101 agattctaat tttgaattta aaaatgctaa aaaagcaaaa cctaatcctc ctgctgctcc
    20161 tgctccttct acattagcta catctttact taagtaattt ttaactactt tggaaaaatt
    20221 tctaagccca ttatctaact cctcaattat ttctgatgtt gcaccttttt gagacccgta
    20281 tatgtatgca gctccatttt tcccaaacag cggattattt acatcacagg ctattttaaa
    20341 cttacattca tctaattcct ttaatctatt ttctattttt atatgtctta tttcatttaa
    20401 aacttttcca cctaatccta ctaacttgtt attttcatca taaaactcaa accctaaggc
    20461 ttgaagcata cctatcccag catcattagt agcacttcca cctatcccaa ctataaaatt
    20521 tctatagcct ttatttattg cttcttttat tatttcccca acaccataag ttgtagtgta
    20581 taaaggattt ctaagttcag tcggaaccaa tgttagtcct gatgcttgag ctatttctat
    20641 tattgctgta cttgtatttt ttaataatcc atatgttgca tccacttttt tagctattgg
    20701 tccagtaact tctatagtat cttttttacc attcatccct tgaactaagg catctactgt
    20761 cccttcacca ccatcagcaa gtggcaatat ctttacttgc gcatcttctt taaccttcaa
    20821 tatccccttt tttattgcat ttcctgcctc tattgaagat aagcttcctt tcaaagaatc
    20881 tatagaaatt aatattttca tatttatcta ctccttttta ttactcctta ttttaaacat
    20941 ataattttat aagcttaatt tatcttttat tttatactct acatctctct ttgtatacaa
    21001 cttataccat taatattctt ttatcttata aaaagggagt gtctcaaaat aaatttctta
    21061 gttcatgaga cactcttttt gtataaaatc aaatatatgt ttattatttc aacaatgata
    21121 aggaggatta tctctatttt aagataattc ctttttagca ctatttaacc agtatagttt
    21181 taacttggtt cattaagcac tctacattta cagagttatt atctattgat atagattcta
    21241 attctttttc atttaggtta atccatttag ctgttttaat atttttgtta aatactatgt
    21301 tagctgattt tgtttcttct ttagaattgt aaaatctaac tactaatgcc tcttgttttt
    21361 cagccttttt tacaacactt aacacaaggt ccttattagt ttccttaagt aaactatatg
    21421 aataatctac ttctatttct gattgattca atttcatagc attatatggc attttattat
    21481 aagtttgtat ttttgttaaa tactctttag ccatatttgc aacatttgct tctagtgtag
    21541 atttttcatg agtaactatt gctaagtcta tttttatatc tcctatcatt tgagaatctg
    21601 gagtaggcaa ctttattcct gatggacgac ctgggcgtct tgccatttct tctttaccta
    21661 ggaatcctac acttctaaat aatgttatag ctattgtgtc aaatttttcc cctactattt
    21721 caaactctct tgtactgttt gtaagaacag ctactccatg ctctttgtca gacaatccaa
    21781 cgaatgttag cattggatat atagaatctg gtctttcact ccattcttcc tgttgccaaa
    21841 cttccatagc atcatcatac acacttctct ttatatgtcc aaactgatta tcagacacag
    21901 aaaactttga agctatattt gttggtatat aagttctaac cctacaatct cttgcttggt
    21961 tatttatgtt gaatactaca tctattatag ctttatcatt tggaacattt ataactatat
    22021 caaagtcaat aaatgaatct gctctattat tttttctagc ttgtatatct tttggtactt
    22081 ttaatctata atttatcttt atctcattat taaatctatt tttctttata gtggaatttg
    22141 ctactatatt atcgttaaat attaatttct catttggaag tggtgaaaaa tcatactcat
    22201 ctccttcatc tcctccattt tccatcaata atacattttc aaaagtttta tttaattttt
    22261 tatcaaatat ctttaatgtc ccgttttcat taacttgaat agtataaaag tcattatcta
    22321 ccatgtcaca tacttcataa gctttttcta acatatgttc aacttctttt atcatatacg
    22381 ccttataccc cattgcaggt attttatctt taaagtttat tgtatatacc ataaatgaat
    22441 catagtttcc ataatgaaca atttgacggt ctattaatcc tgcatctatt acttctttat
    22501 gtaccacttc aaaatccaac ttattattat ctttatcaaa tatttcaaat gctttcattt
    22561 tagttataac ttgagcgctt actatttcat tcctatcgta aggcattaag ttaaatgcta
    22621 ctaacttatc taaagccatt tcacaactca ttgcatcaac tattcttctt ttgtaaaatg
    22681 ttattaattg gtctactttt tcctcagcta agaaaaatct attcattatt tcatggtgaa
    22741 ctttatctga acaacaacat ccaatgctgt catgagcatg atttttcatt atttctttcc
    22801 aaattaactc tattaatcca tgatgatatt caaatcctag agaatatgca atagaagcta
    22861 gtggctccaa tatgttggtt atctttgatt ctattctagc atttgctgat tttatatcca
    22921 ttcttgttga gaatatactt ctatgaaccc tcatatattt cccatctaaa aattcccctt
    22981 ttattgtatc tatatcctca tttttttcta gctctttaaa tatgttttca tatctactta
    23041 agaaaaattt tctctctgga taacattctt ttaatttata tataacttca aatatattct
    23101 tttgaattgg catttggtca tgtccatttg gaagtattat atggtcagta gttgcaccct
    23161 tatcaagaac cggtaaatat ttatccattc ttgttcttag ctcatcttca ttcattggta
    23221 gatattttcc tattgcataa cctaatggaa gtatttgaac caaaacagaa gacccatcat
    23281 cactcttcca attaaactct gttttattag ttccttttct ttcagaacat cctctccaaa
    23341 acattgagta ttttatatca aacccattta gtatttgtgg catttgagca ctttgaccaa
    23401 atgaatctgg taaatatcct attttcatat attctccaaa ttcatcacaa tcttttattc
    23461 catatagtaa atttctaagt atggattctc caccaactac catttcatca gtttgagtat
    23521 accaaggacc tattattagc cttccttctt gaactaattt ttttattctc tccttacatt
    23581 caggcttaac tgctaaataa tcttctaata tagcagtttg accatccatc acataatatg
    23641 gatagttatg attattttct aacatatcca ttatttcttc catattattt acaagtaata
    23701 ttctagattc ctcagttgag aagtaccatt ctctatccca atgcatatga ggaactatgt
    23761 gtacattttt tttcattgct tatcctccaa aacttatctg ataatatttt ctgtctaatt
    23821 aaaaatccaa taaaaatact actgagttta caaaattttt taatatttat tttatcacta
    23881 taattaaacc tttgatttaa atatctaata acgtatcttt taatgtatct ttctttgatt
    23941 tttttgtttc ctagttatta ttaataatac tgctgatatt atagctccaa gtactgccgc
    24001 aaagaaccat attgatgcta taataaatcc tgacatactt ccctctaaaa gcgccaatga
    24061 gaatatcccc gctccaggca cttgaagacc tatattgaag aatgctacta tagacccagt
    24121 tatggctgaa ccagctacca atgatggtat aacacggatt gggtcctcta tcataaatgg
    24181 tattgcacct tcagttattc cagccaatcc taatatccaa gtttgctttc ctatttcctt
    24241 ttctgattct gaaaataaat ctctgcctaa aacagtagct attgtaactc caaaagctga
    24301 aaccatctta actgatgcaa atattgcata aggtataaaa tttccacttg ctattgctcc
    24361 tattgcaaat gtataggctg ctttatttat tggtccacct aagtcaaatg aaaccatagc
    24421 tcctattatt gcacctagta gtattgcatt tactccagtc atcccattta accaatttat
    24481 aagaccattg ttaagtgctg ctacaggctt tcctactaca aataacatca ataaacctac
    24541 taccaaagaa ccaactactg gatataccca aaaacttacg aatccagcta atgtgccttt
    24601 ggctggtata ttcttcttta aaaattttac tatataacca gcaagtaaac ctcctaacat
    24661 acctccaaga aatccactac ctattaaatc agctgctaga cctgctgcaa atcctggtcc
    24721 aagacctggt ttttcagcta gtgagtaaga catatatgct gataatactg gaaccatcaa
    24781 tgtacctaac atacctccag aaagctttct taattgccat aaccaagaac cttcttgatt
    24841 gtatagttct tgtagaccaa atgcttgaga tgctaatact gcaaatgcaa gtatcatacc
    24901 tcctgccaca ataagtggta ttatatgaga tactcctgta agtattgctg attttacatc
    24961 ttttttccat gatgattttt cacaatatga tatatcagat gatttttcta tataatcacc
    25021 tttaccttgt ttttgtgcta cttctaaagc ttcgtatatt atttcttttg ctctttttag
    25081 tggagctgct actgacgttt ttatgtttgg caaatgttca aatctttctg tatttttagg
    25141 tgctacatct gttgcatata ttattgcatc tgcccttttt attatatctg gtgtatgagg
    25201 gtccaccata ccattagctc cttgcttttc tacatacaca tccacaccga tttctttacc
    25261 agctttaatt aatgcttctg ctgacatata tgtatgtgct atacctgcag ggcatgctgt
    25321 tattgctaat ataatttttt ttgattcatt ttgaatccta atctcttctt ttatttgttc
    25381 tttcttttct tttatatcta agttattaaa taactctttc ttagtttttg aatttaacaa
    25441 catattcata taatcttcat ttgacagtct agtaactaac tcagaaagta tttgtatgtg
    25501 tgtatttcca ccttcatttt ctggtactgc tattaaaaat attatctcta ctttattatt
    25561 tgggtctacg ctttcccaat tttctattgg tgtctctaag gtcatagctg caaatgcagc
    25621 ttctttaact gacttagatt ttccatgagg aattgcaagc cctctttcaa aacctgttgg
    25681 agataaagtt tctctgtaca ttacagcttc taaatattcc tcataagatg ttaacttgcc
    25741 ttcatcatat agctttcttg ctaagaactt tattgcgtct ttctttgttt ttattttttg
    25801 ttttaaaaaa acaaggtttt catttgtgat tttcttaaat tccaaaatta cgccctcctt
    25861 aaatcattta atgctatgaa tatagcataa aataaattat gaaaatgttt ttttgaaatt
    25921 atacacacaa tttcttctta aaaaataaag atttgtgtat ttatacacaa atctctatct
    25981 taaccagtat tgctctatta ttgctctcat aatcatcata attggaacct tatctgatat
    26041 attgtaccca tttacttgtt taattgttga atagcaatat aaacttatat ctgcaatttg
    26101 ttgcaattga ttttcacata tatgtgttag agctactacc ttacaaccct tattaactgc
    26161 tgtctttgct acttctataa cttctttcgt ttctccactt aaactaagtg caaaaaacat
    26221 atcttgttca cctaattttc ctatatcatg aagtacttca tgtctttgac tatagtaagt
    26281 tatattatca tgcccacctc taatttcttt tactaatatc tcacataaag gaatgttttg
    26341 acctattcca taaactataa tactcctaga tttttgaatt atatctgcta ctttatttat
    26401 tttttcataa tctattagct ctaatgtctt ttttatatct gattcaaaac cattttttgg
    26461 ctctttatcc tcatttttta actcttcttt taaattattt tttagttgag agaaaccatc
    26521 atactccatt tttttacata gcctaactat ggtatttggg acagtaaaaa atttatctgc
    26581 taaagattgt attgataatt ttactatttc ttctttgttt tttctcatat attcaattat
    26641 catgtcttct gtatctgtaa atttatactc atattttttt gctctttcat aaaattccat
    26701 agtttctcct ccatctattt tattattcat aagtaaaatt caaaaatatt agctaaaatt
    26761 ttcgttaata taattataat ttaatatttt taatttatta atgcatacta ctctttttat
    26821 tatacataaa aaatgtgtac ttcctatatt gatagagatg attcaagttt ttattttgat
    26881 acatctctat caatattttt ttagaaaata cacatttttt tcaaatactt atttttaatt
    26941 aaaactttag gtaatctatt ctttatatat aattattaga cttgaaaata tctttaaaat
    27001 caaccaattc aatattattt ctttttaacc acattttaac ttccttatca catagcattg
    27061 catgttcata tactctctct tgaataagag aagatgattt tataagacta taatctataa
    27121 atcctggatg agtaaccatc aatgttatat ctttatctaa atttatatat ttttcttcaa
    27181 ataatttaac aaaagattta tttaacttgt aaaaatcatt ctgaggatat ttacttggca
    27241 tgctaataca tgaatccaaa taatagggac aacttggtat accgtattct tttgcagcat
    27301 cacacattgc tgatattgat acatcttctt ctataccatg tatatcaata tactcaggca
    27361 atttactcat atacaacttg taagtttcaa tttgagcaca aacttcttca tatacttctt
    27421 cataattaaa aacctcttta cctgtcagta gttgcgttct tcttattttg ctagaaacaa
    27481 attttccacc ttcatctaac aaattcttta tcttaatatg ttctttgcaa caaggttctc
    27541 ctattactaa attaacatgt aaaccaaaac atacatcatc attttttacc atttctatag
    27601 cttgtcttga aaaaggcata ttcaccatga ttccagcact tgtaacaata ccgttcttat
    27661 aagaagatat tatccctagt gtaacacctt ctgaatatcc tatatcatct gccctaacta
    27721 ctagtttctt cattttatgc gtctgcctgc tttctcaact catcaagttg catttccatc
    27781 tcatccttta ctttaacaac atccgttcct atgatgattt gaactgatgt attactcaat
    27841 ttaattaccc ctttggctcc tgttttttta atattatcat cattcaaaat agtagcatcc
    27901 ttcacttcta aacgtaatct cgtcatacaa ttattaagag tcactatatt ttcataacca
    27961 ccacaatttt taattatctg tttagccata tatgaaaaat taccatgaga aagttttaaa
    28021 ttcttttctt tttcactaac tttatcacct atctctatct caacttcacg tcctggtgtt
    28081 tgcacattat cttttattat tatatattta aatgttacat aatataatac aaagaataca
    28141 actcctacag gtataactaa ccacgcttta tctccataac taaaacttaa gacatagtca
    28201 attatacttg ctccattaaa tgaacccaag cgaacaccca gtaaatatag tacaactcca
    28261 cctagaccag aataaaaaga atgaattata tataactttg gagatgcaaa taaaaacgaa
    28321 aattctattg gttcagttac tgttgccata aaagaagtca atgctccagc ggtcattgca
    28381 ccttttactt cttgcttctt tttatcttgt gctgttttat atatagcata agaaatagca
    28441 ggtattgaaa acatcataat tatgtaaaat cctgtccaga agaatcctgc atttgggtct
    28501 cctgccataa atctaggaat ttctcctctt ataacttctc ctgttgatgt tgtatattca
    28561 cccatttcaa ataatatgta agcatttaaa acttgatgta acccagttgg tattagtgca
    28621 cggttacaga ataaaaacaa gaatattcct attgcaccag ctcctcccaa ccaaaaacca
    28681 aattgattca ttgcagcttg tggatatatc cataataaac taaatatata tgataaaatt
    28741 aaagcaacca ctggtgatag tgtaattaca catttttctc ctgcaaagaa gcttaaaaat
    28801 gctggaactt taactttata tgttttattg taagtccatg ctatacaagc tccaacaatt
    28861 atcccaccaa atacacctgc atttaatgta tcattcaata actctaaatt tgacttaaat
    28921 atcatatatg caactacggc tgcaaccatt acacttgtct tgtctttaga ctttgagtag
    28981 gcactagctg ctcctaataa aaataagtag tccacatttg aaaatactat attagatata
    29041 ttagatatag ctggcacatt gagcatattt ggaccaaata ataatcctaa tatacctgct
    29101 gctggcatta cagctattgg cactagcata gtctttccta aaagtgacag ttgttgcatc
    29161 attttgttta agaacctctt catatccaat cccctttcta tttcggttta attttttgtg
    29221 aaaacgattt attcacattt tatattttaa atattatatt tgtttaaaca aattgtcaat
    29281 attttttatt cattttattt ttatcattta gtttaacata taaatccttt taagtgtatt
    29341 gctatatcta gtctattttt ttaattcatt ttttttattt taaaaatatg tttaaccatg
    29401 tatttgaaac aacccacaaa ataattctat tttgtccttt ttatggttga tatgattcta
    29461 ttttcactat acatgtctaa acacatttga tataatttgt tcatatcagt tcaataatat
    29521 tactaaagac ctttaaggag ggataggggt atatggctaa aaataaattt tcagagatat
    29581 atgaagtatt gaaagaggaa atacttgatg gtaaatatac aagtaatatg atgctgccta
    29641 ctgaacttca acttattgaa aggttttcat gtagcagaaa cactgtacgt agagcaattt
    29701 ctcaattaaa cacagaagga tatgttcaaa gtataaaagg caagggtgtt gtagtgttag
    29761 aaaatagttg ttctaatgat ttttttctaa atatgcacaa tttcaaaggt gtagaatcga
    29821 ttgtagaaga taaaaaggtt aatactgcta ctagtgttct ccacttcagc aaaatcttaa
    29881 ttgataataa gttatctaaa aagacaggtt ttaaagttgg tagtgaagta tattaccttc
    29941 acagactaag atatattgat aacataccta aaattctcga cattaactat tttctatgta
    30001 gtatagttaa agaccttgat gtttctatag cacaaggctc tatttataaa tatatagaag
    30061 agtgtctagg tacaaaaatt gtttcatcca gaaagatatt taaaattgaa aaagctacag
    30121 agcttgagtt aaaaacgcta cctctaaatg attataattg tgttggtgtt attaaaaata
    30181 gtgtatatac tgatgatgga aagctttttg aatatactga atcaaagcat acacctgaaa
    30241 catttgtatt catggatgtt actcaaagat attaaaaaaa ttatgtttaa ttaaacataa
    30301 tttttttacg tttagacact ttatcccata ctaacctcac tattctatat acaattatgt
    30361 gtttatacaa ataattattt attgtatatt ttagctttaa tattagttta atgcttgaca
    30421 atttgtttaa acatattata attaacttat aaattacagg aggtatcaat tatgaaaaat
    30481 tggtggaaaa aagcaactgt ctatcaaata tatccaaaaa gtttcaaaga tagcaacaat
    30541 gatggtatag gtgatataaa tggtattata gaaaaattag attatctgta ttatttaggt
    30601 gttgatttat tatggttgac tcctatgtac gtttctcctc aaagagataa tggatatgat
    30661 atagaagatt attataatat agacccaaaa tatgggacaa tgagtgactt tgaaaaactc
    30721 ttaaaagaag ctcataaaag agacataaaa ataatgatgg atatggtcct aaaccataca
    30781 tctactgaac ataaatggtt taaagaatct aaaaaaagca aagacaatcc atatcgtgat
    30841 tattatttct ggaaagatgc taaacctgat ggttctgttc ctaataactg gatatccaga
    30901 tttagtggaa ctgcttggaa atatgatgaa accactaacc aatattattt acatttattt
    30961 gaagaaactc aagctgattt aaattgggaa aatgaaaagg ttcgtgaaga atgttacaaa
    31021 atactagaat tttgggctga taaaggtatt gatggttttc gtttagatgt agtcaaccta
    31081 ctctccaaaa cacctggatt acctgatgac cctataacag gaccaaaagg agatggaaga
    31141 actcactacg ctgatggtcc tcgtatacac gaatatttac ataatatgaa tcaaaaagtt
    31201 ttcaaaccta aaaatatagt tactgtaggt gaaatgtcat ctactacacc tgaagagtgt
    31261 atcaactata ctagagaaaa tcgagaagaa ctatctatgg tttttagctt ccatcatatg
    31321 aaaacagatt ataaaggagg cgcaaaatgg actaatgaaa tgttctctct ggataaatta
    31381 aaaaaagcac aatctgactt tcaatataaa atgtatgaag gaaaaggatg gaatgctcta
    31441 ttttactcta atcacgacca acctagagct ttatctagat ttggagatga tagatttttt
    31501 cgtcaagaaa gtgctaagat gttagccata acactatttg gtctacaagg tactacatac
    31561 atatatcaag gtgaagaaat aggtatgaca aatgcctact ttactaaaat agatgaatat
    31621 gatgatgctg aaagtaagaa tgcatactat catatgatta aatctggtat tgatgaaaag
    31681 gaggcattgc ttatacttca acaaaaatcc agagataatg cccgtactcc tatgcaatgg
    31741 gataatacta tttataaggg gttttctaat cataaaccgt ggttaaaagt taataatgac
    31801 aatattagtg tagaaaatga gcttaatgat tctcgttcag tattttatac ttatcaaaga
    31861 ttaatcaaat atcgtaaaca atatgatatt tttacagaag gtacttatag attgttagat
    31921 gaaaatcata aaaatctatt tgtttatgaa agagagtata aaaaccaaaa tcttcttgtt
    31981 ataagtaatt tcactcatga taatgtagta tataaattac ctgaaactta tttgaataaa
    32041 ttaaactaca caatacttga aaccaattac actagagata taattgaaaa taaaatggaa
    32101 ataaaacctt acgaaagtat aatgatatat tataaataaa gttatttatg taaaaatagg
    32161 tataacaccc tggtgttata ccaattttac taaattcaaa gtaatgttta tagaatatct
    32221 atagattaca ataattattt gacttctaca tcattatatt tctttgaata tactttagtc
    32281 atatagaaat atataggaac tacaattatc atcccaccta atccccaaac tatcttcata
    32341 ggttcatcaa tacctgcttt tatcagtaat aatatactta taataactgc aaatattggt
    32401 ataattgctc ctccaggtat tctaaaattt acatctttac cagcatactt ctttctcaat
    32461 attagtacag ctaaacatgt aggaatatat tgaataaatc tagatacaac acttatttga
    32521 gataatgttg tgaatgaacc cgaccatgct attagtaaag ttcctatgac agatactata
    32581 attgctatat aaggagctcc atttttatta gtttgtctta tagacttagg cattaatcct
    32641 tgttcaacca aagcagcagc agaccttggt gtaaatatgg atgatgcaat atttatacct
    32701 ccaacagata ctatagttcc tattgtaatt atatacccac ctataggacc taacatctta
    32761 atggcagcat ctgcaacagg agtagctgac cctgctaagc cattacccaa cacccctata
    32821 cttattccca gtataagcat atatattact gatactacaa acattaccat taccaatgct
    32881 ttaggtacat ttttcttagg gttctccata tcttttgctg caacagctaa agattcaaat
    32941 cctgtaaaag cataaaataa agttatagat actgctacaa aagctgatga cgcactttga
    33001 cctttttgta ttattacaaa tggggtaaaa ttagaaccct ttataaagaa taatcctaca
    33061 gctataaata aaactattgg taataatttt cctatagtca tgatattatt taagtgctta
    33121 gattgtttca ttcccataaa atttataatc ccaagcccta caactaatat accaattatt
    33181 atatttttaa taagtggttc tgctgcttgt ggccatacag atgatagtgt tacagcaaag
    33241 aaatttgcca tagtagccca agcaatcata ctaactatcc acttcataaa tccaacttca
    33301 aatccaacaa aatctccaaa tgcctctttt gcataaagat agggccctcc atcagtatca
    33361 aaccaactac ctgcttcagc aaaacataaa gcaatagaaa cagctaaaag catacaaaaa
    33421 actaatacta atatacttgc tggacctata tctgtataag catcaccagg taacccgaat
    33481 atacctgacc ctattattgc attgattcct aatagtacaa tactccaaaa acctaactta
    33541 atgccttttt ccattgttat tccccccaaa gccaacttaa tatatttgta ttttttattt
    33601 agatgcttct ttaactattg ccataaatat ttttttcata ttatcataat ctctagtcat
    33661 catttctgga tgccattgta tccctattac aaactcactt ccttcctttt ctatagcttc
    33721 aattaaaccg tctttagatg tagctgaaac aatatatccc ggtgctactt tgttgacaac
    33781 taagtgatga aaactattaa ctaaaacttc tttttctcct agtatttcat ataattttgt
    33841 cccctccttt atctgtactg tatgtgtagg tacattagat agatgttgct gattgtgttt
    33901 aatataagca ccttcaatta aagataaatc ttgatacaaa ctacctcctt cagttacatt
    33961 tataatttgt tctcctctac atatacctaa aactggtttt ttcatatcta aagcatgttt
    34021 taataatttc atatcaaaac tatctctctt tggtgatatt gcccccaact tattatgagg
    34081 ttcttctttc catatgagtg gatttacatc ttgtccccca gatagtataa gcgcatctat
    34141 attagatacc tgctccttta taatctcatc atcatataca atcggtacaa tatatggtat
    34201 agctttacac ataactactg attgtatata atcattattt acataagccc tttcatagcc
    34261 aggaaacatt cctccttcat caattagtaa attacctgat attcctatta ttggatattt
    34321 ctttttagcc aatttcttca ccccttaact tatttgtgaa caataaaaaa tgctacttct
    34381 cttaaagagc aagtagcata ccaagatttt atttatttga acattataat ttattttaga
    34441 aatacttttt ttgaatacga gtaatataaa taactttatt tacttatatt ttattttaat
    34501 cctgtttagt ttgacttttt taaattgttt taagtacaac taataggtaa aataggcatt
    34561 tagattttcc tttttatcaa cctattaaat actgtttaat atattttttc cccttctatt
    34621 taattcactt ccctttctac cagattcaca tattatcaaa tccagctctt ttaacatatt
    34681 aagtattcct cttacctcac cttctgttat attgtagcct aattcattta ttgaattaac
    34741 aataccattt ctacctatat ttttattaag attattggca ttttttaaac actccattac
    34801 taactttact ttctctatag atgttctatc ctttaatatg cttatttctt tactaaaatc
    34861 ttcagttata tttaataagt tatgaggtaa atcatttatt aaaataacat cctcagaact
    34921 cataatgtta atatacatag cagtattttt taattctctt atattacctg gccattcata
    34981 tgcattaata atgtcttgaa catcttgact aattgctctc ttccttttca taaaatgctt
    35041 taacataact tctatatctt cttttcgctc tcttaaaggt ggtatattta tgggcaatac
    35101 atttatccta tagtataaat ctgctctaaa tctatctcct tttatcattt ctaataaatt
    35161 tttatttgta gccgcaatta ctctaacatc tatatctatt acattttctc ctccaaccct
    35221 cattacttga ttctcttgta atactctaag taatttgctt tgtaagtata taggcatgtc
    35281 tcctatttca tccaaaaata tagttccatt gttagcctgt tcaaataatc cttttttacc
    35341 tccttttaaa gctcctgtaa aagcaccttc ttcatatcca aaaagttcac tttcaagtaa
    35401 attttctggc atagctgcac agtttattgc tataaatggc tgactatgtc ttggtgatgc
    35461 attatgaata gactgagcca tgagttcttt tcctgttcca ctttctcccg ttataagaat
    35521 tgtataatca gattcagcta ttttcatacc taatttctta gttctttcca tcttcgttga
    35581 aactgttttt atatcatcaa aagtatatct agcaacttgt cccttttctc taagcttctt
    35641 tgataggttt tgctccaatt ttcttatgta agttatttct tgaatacaaa aataatatcc
    35701 agtaaccctt ccaaaactct ctatattata cttatttacg tttaagtact tattattaaa
    35761 ttttataact tcatcatgtg ccttactcaa actaaaaatc tttttaatat tctcattctc
    35821 taattcatct atatttttcc ctataatatt actttgaata tctagtatat cccttaaact
    35881 attattacaa ataattatgt ttcccttatc tgataccatt gccataccta agtttgataa
    35941 atttattatg gaatttagtt catctgcttt tatagtaagt tctttgtatt tagtatttat
    36001 tcctgaatat aaactaatta tttcatctgt atacttaatt agcctctcag taatttcttt
    36061 atcatccagt ttgagttttg ctattatttg aataaacgtt gaactatcaa tatacctatg
    36121 gcctaaatca ataacttctt ctatatattt tggaactaaa cttgactctc ctggcgttat
    36181 agctatctta atatttctat attcttcatt gggattgtat ggtataaaat tcaaatgatt
    36241 tattcctata ttgtataaat tagatattgt ttccaataca gtatgcttat tatcgtttac
    36301 tactaataca tctattcctt ctggcaatga aaataactta tatatatcct tttgctttat
    36361 gctcctattt atagttatta tctttgagac atcttccaca tattttttta tttttaatac
    36421 tctatcatct atcattacca atattatgtc atcttttatt aattccttct ctgttatttt
    36481 atttaaataa taatttttta tcattacttt atctttaaag atcatttcta gatttttatt
    36541 taagaaacat tctaatttag acaacccatc agttactact gctattgttt tcatttgatt
    36601 actcctttta aaatatactc tactctttta taatatacat ttgattttca ctttaataac
    36661 acaaaaaatt gtacatttag tatactaaca tattccttta ttagttttat gtccataaat
    36721 atcatagttg caatatctat catatttctt aaacttctat aaagcaaaaa ttgtgccaaa
    36781 ttaataattt tcttaatttt attctaaagt tcctcaatag tatttacaga cttagcactt
    36841 atagcttata agtctttata ttattaatct atttttatag gttaattgta caaatcatat
    36901 atcactttta cctattaaat gcattataaa aaattgtatt tataaacact atttactact
    36961 cttctaagta caaacagtat ctacatacta taacctttca ccattagatt ctattacttg
    37021 tttataccag tcaaaactct ttttcttaat cctagacaag tctccacttc catcatcata
    37081 cttatccacg tatataaacc catatctctt ggccatctca ccagtagatg cacttactaa
    37141 atctatacat ccccatggag tatatccaaa taaatcaacg ccatcttcta cagcttcctt
    37201 catttgctct atatgtgctt ttaaatactc tatacgatag tcatcattta tcactccatt
    37261 agattctttt ttatcataag ctcctaaacc attttcaact accatcatag gtattctata
    37321 tctatcataa atttcattta gtgcatatct aagcccttca gggtctattt gccatcccca
    37381 ttcactagac ttaagatatg gatttttcac tccacctaat atgttgccac ttgtattatc
    37441 ttctttagaa tcactactta cacatgtaga catataataa ctaaatgtat aaaaatctac
    37501 acaaccttct tttaaaattt ctaaatctcc tttttccatt tttatttcaa tattattttc
    37561 tccaaaatat ctattcatat aagttggata ttcgccacgc acttgtacat ctccacagaa
    37621 ataattcatt actctactat ctctttgagt tttaactgca tcatctggat tacaagtata
    37681 aggatacatt gttgcaaata tttgcataca tccaacttga taatttgaat ctatttcatg
    37741 agctaactta acagccttag cacttgctac aaattgatga tgtaaccctt gaaaacgaag
    37801 ttgaggtata tctacttggt ttataaaatc tgtagtccct tcatttaata ttccttgtga
    37861 taagtagcct cccataggca ttgtagctga atttatttca ttaaatgtca gccaatattt
    37921 tacttttcct ttatatcttt taaatattgt ttcacagtaa ttcatataca agtctattac
    37981 atctcttgaa acccatccat tgtatttctc tgttattgca aatggtattt catagtgaga
    38041 tattgttatt aatggttcta tattatattt taaacattca tctatcacat tttcataaaa
    38101 tttaagtcct gcttcatttg gttctttctc cattccagtt ggaaatattc tagtccatgc
    38161 tattgaaaat ctaaatacct taaatcccat ttctgcaaat aaagctatat cttctttgta
    38221 tctgtgataa aaatctattg cttcatgact tggataaaat gttccttttt ctataactct
    38281 cgttattctt ttacttactt tttgagaacc tccagtacac atatctgaac aagaaggacc
    38341 tttcccatct aaattccatg ctccttcaca ttgatttgct gctgttgctc caccccataa
    38401 aaaatctttt ctaagtgcca taattctacc tccaataatt tatttatagt aactttattt
    38461 taaaataaaa atacatatat ataattctta attaatagaa ctctctttta cgctaaaaca
    38521 gttatcaatt tctgactatt atttacatgc ttttgtgtat tttctacaat atccacataa
    38581 tcagaataat tagtaacaac tactggtgtt tctaagcaat atccttcttt tgaaatactt
    38641 tctatgtcaa aagttattaa tgtctgacct tttttaatct tatccccttg attaacaaaa
    38701 gatttgaaat atttaccttc caactgaata gtatttaacc ctatatgtat caatagttca
    38761 caaccattat cagatataat tcctattgca tgttttgttg gaaataaagt cattacagtc
    38821 ccatcaaatg gtgccactac ttttccttct ttaggtgtta ttgctacacc ttttcccaat
    38881 atacctgttg aaaacgctgc atcttgtatc tgctccaaag cctttacttc accttttatt
    38941 gggctatata ctacttctct tttgtttttt tctataacgc cttgcccatt atcatcttta
    39001 gaaatttcta ctatagcatc atctttccaa aaaaataatg ttaaaacaaa tcctactaca
    39061 atagctacta ttattgctat tatagttgca taaacacctt tcatatctcc agttgttgga
    39121 tttatataat taggtatacc aaatattcca agtccaccca ttacataact ttttacattc
    39181 attatcccca ttacagctcc tgccacagca ccacctacac aactatatat gaatggtgtt
    39241 tttcttggta aagttattcc atatattgca ggctctgtta caccacatat ccctgaaata
    39301 actgcaggta tacacagctc ttttatcttc ttatctttta atttaaagta cattgctgct
    39361 actactgctg tttgtgcaaa agatggtaca aatactccca ctaatattgt gtcaaacccc
    39421 atagtcataa ggtttgacat agctattggt actaatcccc aatgtagacc aaatattaca
    39481 agcacttgcc acaataaacc agttaatgca ccacataaaa ttggtgaaaa gttaaataaa
    39541 gctaaaaatc catttccaac tatattagaa gcataggtag ctataggccc tataaccata
    39601 aatcccaatg gtaaagaaac taataaaact gccattggta caaagaaatt ctttactaca
    39661 tctggtatta ttttagcaaa cactttctcc aacttagctg ctaataatac tattaaaatt
    39721 actggtacta ctgttgaagt gtagttcatt gctattactg gtaatcctaa gaaagttata
    39781 tatactggtg attcaaatat tgttccagta aatagattgt ataaaggttg acctgtagat
    39841 gctagagtat ctaattgaat acctgaatag cataaaactg cacctataag cattccaata
    39901 aatggtttta atttgaattt cttagcagct gtatatccaa gaattattgg catatactgg
    39961 aacattgcat ctccaattgc atttaacata atatatgcac catcagtttc tccatacaaa
    40021 cccaatgctg taaatactgc attaaatcct tttatcatac cagcagcaca cagaactcct
    40081 aatattggtt gaaaaactcc tgatattatg tctataaact tattaagtaa tccttttggc
    40141 gcatctgagt ctgaattatc tattgtatct cctcctaatc cagccaaatc gcacacaact
    40201 tcataaacat ctggaacatg attaccaata actacttggt attgacctgc acttttcatt
    40261 acagtaacta taccatccat tttttttagc acatcatcat ttgctattga ctcatctttt
    40321 aatttaaaac gtagcctcgt tatacagtga gataaactgc taatattatc ctttcctcct
    40381 acattcgcaa tgatttcttt tgatagttgt tcatactttc tcattttttc tcctccattt
    40441 tctttttgaa atatgaaggt ttggccaact taatgtcacc acccaatata tataaaccta
    40501 attattagga tttatatccg acttaatact tttacttacc actctttcaa cgtgaatagt
    40561 taagtataat ttttcctcat ctgttaaatc gtagttgtat tttttatgta taaattcatc
    40621 caacttttct atgaatttat aagcattttt atatttaact tttattacat ttaataaatc
    40681 atcttcatta ttttcatgta aattattact aacaattcgt tctgcaaaat actttaagtg
    40741 agtaataaat ctatgataat aaactgaatc ttcatcaaat actattctgt aatggtattt
    40801 aaccatattt gaaatttctt gtattatttg tgtaatctca tatatagttt ttatactttg
    40861 atccatttgt gcattaacta tatgcagtgc tataaatcca gcttcatctt ctgatagtct
    40921 aatttcaaat tttttttcta tataatccaa tgcctctaac cctattttaa attcactctt
    40981 ataaaatctt tttatatccc aaagtaatac attttttaca cttactcctt cacgagctct
    41041 ttctattgca gtatacatat ggtctgttaa agaaatataa atactatcat ttaatctttt
    41101 ccctagtttt tctttagcat ataaaattat ttcatctgat aattccatat atcttatagg
    41161 aatatctgct attagttctt taaatttatc agatatattt gggtctgaaa gtttgaatat
    41221 cttatcaatt ttatcctttg atatatgctc tcctgtacgt tttttataag ctaaacctct
    41281 ccccataact actatttcct tatgattttc atcagttgta ataattacat tattattgag
    41341 tattttttca ataatcattg tgatgccccc tttattagca ttaagttaaa ataaatcttt
    41401 tttaattatt tagaatattt aaaatgtaat ttattacata aaaaaaaacc tatttattac
    41461 acattaaata gactcccatg agtcctgagt aataaatagg tttagcttgt atttaaacaa
    41521 tcactatccg tttgtatttt attttaatta ttatttatta tatgttagca aatttatttt
    41581 ttagtgtcaa ctttttctca atattttaat tttaaataaa ttatataaat tgaatcttca
    41641 aagtactatt tttatcaata aacaaaataa aaaaggtatt actttcttta agtaataccc
    41701 atttatttct aatttaagtg gtttttctta atctcatcaa gtagtccatc ttctaaataa
    41761 atttctaatg cacatcctac cattgccttt actgctttgt gaagtttatc ataagcttcc
    41821 tcagaatttg cataatttag aaattctttt tcatgaacat ataccattgg acttatatct
    41881 aaggctattt ctgtatacat agtagggcaa acttgactta cattacctat atctgtagaa
    41941 cctactggac catctttacc ttctaatata tctgttatac ctgcctcaat caaattattc
    42001 tttgttatct tttgaagtac tttgttattt attatgttgt caaaagaatt ttcaaagtat
    42061 ctataagaaa gcttagctcc tgtcattaac tctgcacctt ttgcacagtt tataactttt
    42121 ttagttactt catctaaata acttctttca gcagctctta cgaaaaactt acaagctgct
    42181 ttttctggaa ctatatttgg agcctctcct ccatttgaaa ctataccatg tattctaaca
    42241 tctgatttta catgttgtct tagtgcattt atcccagcaa aagtaagttg tactgcatct
    42301 agtgcattga ctcccatatg agggtgacta gctgcatgtg ctgatacccc ttcaaaacta
    42361 aactctaatg aatctatcgc caaagcctta cagtttatat tattaaaagc ttctatatgc
    42421 atttgataac ttacatcaac atcatcaaaa gctcctgcct taactaaatc acattttcct
    42481 cctgttgttt cttcagctgg tgttcctatt agtacaattt ttcctttaaa attttctttt
    42541 aacttactta atactataca agctcctagt gtagatgctg caatccagtt atgtccacaa
    42601 gcatgagctg gttctttatt ttctccatat ccaggaagtg catcatactc agctaaaaat
    42661 gctatggtag gtccatcatt atctcctaat tcagctctaa aagcagtctc catattacaa
    42721 tatggataaa ctgtatcaaa tccatattcc ttcatttttt ctactaagta ttttgatgat
    42781 atgtattctt cttcccctaa ttcaggattg ttaaatataa aattacaaac ttcttcaatt
    42841 tcttttccaa gttcattctc tatctcataa gctctcttct ttatactctc tatcataatt
    42901 tacctcctac tagaatccta gatataatcc tattgtcaaa aatgccatta caagaattgt
    42961 taatactacc attaaaggaa ttacgaatct aacccacttt gaccaaggaa tattggctac
    43021 tgctagtgct cccattaaaa cacctgatgt aggtgttatc aagttagtta cacctatacc
    43081 aaattgataa gctgtaacta tcatttgtct tgatacatct actaagtcgc caagtggtgc
    43141 aagtacaggt attgtaagtg atgcaagccc tgatgctgat ggtattaaaa atgctattaa
    43201 tccttctatt aagaaagtta aatttataaa tatagttttt ggtaatccat ttaataaatt
    43261 tgctgcagaa tttaaaattg tatctattat aaatccattt tctgctatta ttacaattcc
    43321 acgtgcaaat gctatacaca atcctgctgt cattaaatct cctgctccac ttataaatga
    43381 attaactata tcatcttgtt taagacctga tgctatacct gatattatac ctatagctgt
    43441 aaatatcatt gcaatttcag ttatatacca tcccaacttt aaaactccat atatcataat
    43501 cgccatacct attgtgaaaa ttgctattac catggcttct ctttttgtaa attcctttat
    43561 atttgtatca ctattaacca ttgattcttg atttagtgat atatcgtgta ctaaagattt
    43621 tttagggtct tttttaactt tattggcata catcataaca aatcctatag atatagccat
    43681 atatattata aacataacaa ctctaaactc tattccagaa cctggtgcta tttgtgataa
    43741 tgcttgtgct attcctacag agaaaggatt cgtagtagct gctccaaatc cagctgttgc
    43801 tcctataaat actgtggcca ctgctgtcaa tgaatcatat cccatactgg tcataagtgg
    43861 aataaatatc atataaaaag gtagtgtctc ttcactcatt ccaaacgtcg tcccacctag
    43921 tccaaataga atcattgaaa tcggtattat taaaaattga aacttcttca ttttatttac
    43981 agtatgagct ataacagctt ctatagctcc tgttttatta actatcccaa aagctcctcc
    44041 aactattaaa acaaatccaa ctacttcagc agcatctata aacccgttta taggtgctgt
    44101 aaatacatca gtaattcctt gtggatttga tggtgctttt tcataagttc cagctacaac
    44161 aactgacctt ccgtctatat ctactctttc aaaatttcca cttggcacta tccaagtaag
    44221 aattgccatt aacactatta gcaaaaatat tattgtaagt gtctgtggca tagtaaactt
    44281 tttctttttt aaactgctca tttttaatct ccccactttt taaattaaat attttattat
    44341 tgcatccctt tatcctattt tgtatagatg taaaaaagct caacatggat acatgttgag
    44401 ctaaatgtgc taaatacaac atacctgcat cttcccttta tcttgtttaa gatagctctc
    44461 catcaaaatt cgggataatt ttgatgacag tactgtacct attaaataca gtcccagcac
    44521 tagcttcgga cactagcact tcggcactta ctccttttat attgcctcat tggtttccaa
    44581 cctcttcaat attaatctta gtaaatgcaa cctctacctc aatctgtttg ttagatgagg
    44641 taattcttat taatttttat taattcaaag taattgtagc ataatatgtc gagattgtaa
    44701 atattttcag tataataatt cttactttat gatttcattc catttttcac aaataatttt
    44761 ttctccatat aatttaaata ctgtagtata ttttaaatac acacaaactt tccacaaatt
    44821 ttaaattatt ataatacctt tataataaaa aataaaagct atgtaaactt atcaatttac
    44881 acagctttat atacattaat ctactccaag tacagcttta agctttgatg ctattgtaat
    44941 aactcgtgga ccaaatataa cttgaactga gtttttcttt ttaacaactc cacttgcacc
    45001 caattcattt acccagaagc catcttcata gaatccatca attgaattta gctctaccct
    45061 aagtctagtt gcacagtttg taacattttt tatattatta cttcctccta aaccttctat
    45121 tatagaatgt acaagctttg tatctttatc atctacttcc tcttctactg cattgttgta
    45181 tgctgtttta ccagcatcta cagattgtct ttctctatat tctttcttac tataaagctt
    45241 aacttcttct gaacttcttc ctggtgtttt tatatcaaac ttcttgattg cccatgtaaa
    45301 tattatataa gtaactataa aaaatattgg tatcaaaaat aataatggta acgcatccac
    45361 tttttgtggt tgtaataaat taggaaacat atttctaaat gattctccca ttatagaaac
    45421 attaaacatt tctgtaaata catacgtcaa tcctgccagt ggtacatatg ctacaaaata
    45481 caataatggt gcaacaaata ggaatgtata ctctattggt tccgttaccc ctaccaacat
    45541 ggctgttact attgctggta tcaatatact tattgctttt ttcttattag cattatcagc
    45601 caacttatac atagctaaag ctgctgctgg taatattgca aaatgtatta ataaacgacc
    45661 tgttgtgaag ttacgagtta tataagatag tgtatctggg tctcccattt gagctaatct
    45721 tatattatta acaccctcgt aaattgctcc acctacttcc attgttccac ctactcttgt
    45781 atattctata gggaatgcaa ttaagtgatg tataccaaat ggtaataatg ctttatccat
    45841 tgctccaaaa gtaaatgttc ctaataatcc actagaacct atccaattac caagtttaga
    45901 taatgccaac cctactggtg gccatataaa gtacattgct gtacccaata ctattgatgc
    45961 tataaacatc ataatcattg aaaatcttgg acctgagaaa aaagataatg cactaggaag
    46021 ttctatgtca acaaatttgt tatgtactgc actagctaca aatccagcta ttatagctcc
    46081 aaatatacta caatcatatg aaaagattcc taagaaagaa ccatataaag aacttcgact
    46141 atatgcttct gcctctccta atccagatgc cataaacgct tctgctgtaa ctgtatctga
    46201 atttatacca tttgctgcta acaatgtact tataactgtg tgcattccta taaataaaac
    46261 aattcctcct aatgcacccc atcctttttc ctttttagat aatccaaagg ctatacctgt
    46321 aacaaagaat attggtaagt atctcattac cataaatcct aaatcgttta ttactttaaa
    46381 gaatacattt ataaatgacc cttcttttat tagtccgcta agccctaatg cagaaacgtt
    46441 ttccatattt gcaaaagctg accctagtcc tataaatact cctgctacaa caagtaatat
    46501 aacaggtatc atcattgctc cagaaagtac ttgtaattta tccttcatag tattatttcc
    46561 tccattttta ttgaaatgcc aggctatata atgcatatat atatacttct attgctttgt
    46621 ataatgtatc tatacttata ttttcatttg cctgatgtga atttggatta tccccctcta
    46681 ttgtgggtcc aaatgcaact ccatttttta acactcttgc ataagatgca gctccttttg
    46741 taaaaagttc ggcttctttt ccaaaacctt gtttatatgc atttgataat ttatttatta
    46801 attcactatc tggttttaca tacagtaatt ttaaatcctt atatatatca actattaaat
    46861 tttctttttt tccaatttca ttaattctat tagttataaa ctcccaactt atacccttag
    46921 gatatctaaa gtctattttc atattcaatt catttttctc taagaagata ctcattatac
    46981 atattgtaga aactcccata tctacatctt ctttatatat ccctaatttt tttccatgat
    47041 tatcatctgt aaagtatgaa tttaaaatat cccttattat aacgccttta tcatttagtt
    47101 ttttaatttt ctctaaatct tttgctaaac taaatgcaca gttatatgac ctgtgtggat
    47161 ttctcgataa agccctttct cctgtatatc ttactaatac tttaccttct tctagttctt
    47221 ctatctctgt ataataagct aatgattcta ctaaatcttt cttatcatta gttttaaaaa
    47281 cagcctcaat tttgtcacat acaaatccat cttctttgtt tgactttata tcaactaaat
    47341 tgtgatttct aaatttatct ttatctattt tctttcttag attgaagtat aaaatacctt
    47401 tttctccatt tactattggg aagtttccat ctggagaaaa tgcaaatttt ggctgttcat
    47461 tgacactaaa gtaatgctcc atacattccc aagtcgtctc ttcagctcca cccactatca
    47521 atctaatctt tctcttcggc ttttcattaa gttctcttaa gaaaagtaat gcatataaaa
    47581 tccctatcaa aggtcctttg ttgtcattaa ctcctctacc ataaagatag ttatcttttt
    47641 gacatacttt aaaaggttta ctcttccaaa gttctttatt atatattggc accacatcta
    47701 tatgattaag cacccctact acttcttctc cttctccata ttctatatga attgcatatc
    47761 catcaaagtc ttttacaaca aaatctttgt cttttgctat ttgaataagc ttatcgaagg
    47821 catttcttat ttctatccca aatggttgat ttatatctgt tgtactttca tctctaattg
    47881 aaggtatttc aactaaactt ctaatatcat ttatgatatt ttcttgattg gattttaaat
    47941 aatcaataat tagttctttg tttactatca tcttaattat ttatcacccc tttatattaa
    48001 ttttacaaag catttcagtt catatctaca ttataaattt tgaatattat tttttcaata
    48061 tttcaaatac ttctctaagt taccataatg atacggtaaa caatcaacag attatgacaa
    48121 aatcatacta tacctattac taatatttta acttatctat tacgtctttt ttaatgtaag
    48181 tttattccaa aaatacataa aaaaagatgt agccttagct acaccttctt atatctattc
    48241 aacttccatt attatgctat taatatctac atttccaggt tctatcctat cttttactgt
    48301 tctcttaaca gaatcagcat catccattat aactacagga gttattatat ttaaatcatt
    48361 tgacgttata aaatctaaat ctacttccat tattaaatct ttagctttta ccttatctcc
    48421 tgcctttacc ttaacatcaa acggctttcc ttctagtgac attgtctcta aacctacatg
    48481 tattaatata tttgtattat cattactctt aatcattatt gcatgctttg tatcaaatac
    48541 attgactatt tcaccatcaa caggagaata taacttacct tcacttggta ttattgctac
    48601 tccatctccc atcatctttt gggcaaaaac tgcatctgga acatcttcta agttcactaa
    48661 atttccctta aaagggctat atattttctc aatcttattt tcttttttct taaatatatt
    48721 aaacataaca cactctccac attataaaat tttatgataa tatttttctt aaagcttttg
    48781 ctacaccatc atttacatta gtatcagtta caaaatctgc catagatttt acttcatctg
    48841 tagcatttcc catagcaata ccaagacctg caaatttaat catggatata tcattttcat
    48901 tatcacctat acaaataact tctttgctat ctatacctaa tatatcacaa aattttttaa
    48961 ctgcaatacc ttttgaagta cctttactca taacttcaaa gtttatcttt ccagaactta
    49021 ctacttctaa atcttcttgt tttttaaatt cttcctttgc ttctctcaaa gaatctacgt
    49081 tttctgagaa aagtatacat tttaaaattt cattatcagc tttttctaat aaagtttcac
    49141 aacttgcatt ttctattatt tcaattctgt cttcttcttt atttttacta tttattaatc
    49201 tatatggatg ttcttcacct atttcattgt tggatataac tattttacag cctttaaaat
    49261 gtttgcttaa attatacttt tcacctattg tatagatttt ctttagagct tctttgctca
    49321 aaaccttctt atactcatat cccagtaatt taaaatatgt cccattagtt gatataatat
    49381 atatatcatc ccctaaaaca cttgtaaaat atttcatagc attataaggt cttcccgtaa
    49441 ctaaggctac cttgataccc ttttccatga cttcttttag tgctttaata ttttcttctg
    49501 atacttcaaa tcctttaccc atcaaagtac catccatatc agtacaaatt aatttatatc
    49561 gcatttgtat cacatcctag taagattttt ctaatatttc aacaaggtct tctttctttc
    49621 caaaaggtaa aaatcctaaa actggacagc ttgttatttg ttctaattgt tcctttgtta
    49681 cattaaactc agacattttt gtcttaagtc ccactctctt taaaaaactt tctatttcat
    49741 ttcctaaagt tttagctgct tcatcatctt ccactttttc taattcttgg tttagcattc
    49801 tcgctacttt ggcaaatttc tctatattat ccttatagaa cttttctgca aattttggga
    49861 acactatagc caaagcctca ccatgaggaa tatgtgtaac tcctcctatt atttcactta
    49921 gtggatgtgg tgctgctgct ccagcatttg ccagtgaact tcctcctaat gtatctgcta
    49981 ctgctaactt tgttctgtat tctatattat ttttatcttc cattacttta ggtagatagt
    50041 taatgactaa ttccatagat ttttcagaca ttatctctgt aaaatcatta gcatttggat
    50101 ttatataact ttcaaatgca tgagtaaaag catcaaatgc tgttgatgca gtaattctct
    50161 ctggcagagt taacataagc tctgggtcaa ctatacattc ttttgagaaa caattttgat
    50221 gaaatattgt atttttctcg cttcctctcg atattactgc tgcttgtgta acttggcttc
    50281 ctgttccaga agtagttgat acagctatta atggtaattc tttttcactt accttatcgt
    50341 atattttaaa tggatttgta tatgtactaa acatataatc ccaatctatc ttttctaaac
    50401 caaaagtcaa tgctaatact tttgcagtat ctatacttga acctcctcca acagctaaaa
    50461 caacatctac agattctttt ttagctaatt caaacccttc atttattaat tctacagttg
    50521 ggtttggctc tactctatca aagtgtacaa cttctatacc tttttctatt aaattcaatg
    50581 taactctttt atataacttg tctaatggct ctacatttgg agtagtaact aacaaacatt
    50641 tctttccata ttttcctatt attctaggta attcacttaa agttccttgt ccaaaattta
    50701 ttcttgatgg ttgataaaag ttaatcatat ttttcctccc ttaaattatt gccttcatat
    50761 tcaaattata catatcatag gatttttaag gcaatacctt cactgttaac tcttatttac
    50821 taaagcttat ggcaattatt agacaatcaa tgttttatta ttaccatatc attatgtaaa
    50881 ttttattaag tacccatgtt ataattttat aatcaatctt attgtataat tgacttaata
    50941 ataaaaatag agtatataca cttttttatg acaaatagct taaaggagaa ttaaattatg
    51001 aaatatttga tagacagtgc taatcttgat gaaattcaaa agtgtttaaa gtatggattt
    51061 aagggaataa cagctaatcc atctatgtat cttaaagaga aagtaaactt ccatgaattt
    51121 attgagacat gtgctaattt aaatccagaa atattaactg ctgaagtcat tggaaataat
    51181 ttatacgaat taattaattc atgtgataag atattaaata taaataaaaa cactataata
    51241 aaaataaatt tctctgaagt tggcttagaa cttataaaca tattaaatag taaaggtgta
    51301 aaaactgcat gtaccttgat tttcaatgta aatcaagcat cattagctat aaatgcaggt
    51361 gctgattacc tattcccttt tataggtaga aatgatgaaa atgggaatga tggtataaaa
    51421 aaccttacag aaatatgtaa ccttatatca ataaatggat ataatactaa agttgttgca
    51481 gcttctatta aaaatgtatt ccacttaact caagcatctc tttgtggatg tgattatgca
    51541 gctgtaacat atgatttatt taaaaaagct tcttttaacc agcttacaat agatggtgcc
    51601 aatacttttg aaagagattg gtccaaggtt aataaatagt aactaagatt taggagggtt
    51661 tgcattgaac aaaagattat tagatattgc tgaattatta ctcaacagct cagactatat
    51721 tactgtagat actattgccg aagaattaaa agttagtaat aaaactattc gtaatgacct
    51781 tgttatttta gatgaatggc ttttagagtt taatttatcc ctagataaaa aaactggttc
    51841 aggggttatt attctaggta atgaagatat aaaacttaaa gttattagag atatcaatga
    51901 taaatcaaat tgtatatatg catactctcc tgaagataga aaaaggtata ttctaagtaa
    51961 attatttatt aaaaattcta agtttaggat tcgtgatatt tgcaatgaac tacatgttag
    52021 tagagctact gtgcataaag acttggtagt tattcaaaat tttttgcaaa actttaaagt
    52081 gactctagtt agaaaaacca ataatggagt atatttagag ggaaaagaga aagatattcg
    52141 aaaagcaatt tttgaacttg ctacggttaa taaaagttat actgaactta aagaaatatt
    52201 attctcgaaa tctaatgaat gcaaagatac cccttcaaca aaaatattta aggagctatt
    52261 taactatgat tttgagaaat tatctcaaat cactctacac accttacaag tagaaaaaag
    52321 taaccttagt gatgagtatt atataaactt cttaatacat atagctatat gtattaaaag
    52381 aatttcaatt aaaagatata ttaacttatc tgaaggtttt tttagagaat tattagaaca
    52441 tgaatacttt gaaaaatcaa aaattttatg tgctaaccta agtgaagctt ttgacgtaga
    52501 atttattgat gaagaggtct gttatatcct tcttcatata gaaggattac ttaaatcaat
    52561 aaagaaaact actactcttg aacctcttaa aaatgaagaa attacagagt ataatctatc
    52621 tgaagcaata gctgttcatt ggggaagtat actaaattta aacttaaagg atgatttaat
    52681 attaataaag tctcttgcta atcaccttaa atcagttcta cacagaataa actatgggtt
    52741 tactattaca aatcccatat taaatgatat aaagagaaca tttccttata cttataaggc
    52801 tgctaaagag tctaatactg ttatcaaaca gttgatgaat tatgaagtca atgatgacga
    52861 aataggatat cttgctttat atttaatatc agcaatagat agaagcaaat ctccccttaa
    52921 tactatttta atatgtcact gtagtgatgg tgtatctaat ttattagtcc aaaagttaag
    52981 ttttgaattt aatcagatta atatagttaa atctatacct ctatcttcta tttcatttac
    53041 gaactttgat gatgtggact taatactaac cactgctcct gtagactttg aacataatgc
    53101 tgaactgata aatataaatg ctcttttaag taagaatgat atttctagac ttagtacaat
    53161 tataaaaaaa ctatattctg aaaaaaataa aatattatct tataaataaa caaaaatagg
    53221 cttgtctcaa catagagata aacctatttt tcattgtcta aatgataaaa atatatttga
    53281 tttcatagaa aaagtgcctc atgaactaaa aaattcattt tgagacacct ccatttttta
    53341 tatttaagct ttttatttaa gcttttatat tttttaaact tttatattta aagtaaattt
    53401 tattattttt attgagcaca agcattattt atattattaa tttcttcagt attcttacca
    53461 aactttgtag ttacaaatcc tattgttttt attatgagta ataaagatat caatgaatat
    53521 atagatatca taaaattctt acttattatt ccagacgcta ctattataaa tccatctagc
    53581 ataaataata taactggcaa cttcagaaac ttaattacct tattaataag cattgcaact
    53641 aaatcagttc caccagtact acagcccctt attattgcta tccctatagc agttcctacc
    53701 aataacccac ctatcactcc tgctactaat agattgtcag ttatgtgagt ttgtggtatt
    53761 acttttaaga ataatgggcc cataagagtt atatataatg tttttattcc aaaacctttc
    53821 cctaaaaata ataatcccat tactaaaagt ggcatagata ttcctaatga tatattctca
    53881 acaggcattg ataagaatgt gctaagtacg actgataatc ctgttatccc tcctggagct
    53941 aatgtatgtt ctgcaaagaa aaaattaact gctaatgcca ttaaaaatgc tgatattgtt
    54001 atcagtaaat aatcttttat catttctttt ttcatttaat aacctcctat gattatatta
    54061 tttcagtata aaaaatcaat taaattagtc tagcttcctg tagtttttca taaaattaat
    54121 gcttccttaa aacattaaga aagcatctct tttatcatag tttattatct tattctaaac
    54181 caaactttgt agctgcttta tctatagctt ttctacaatt cttaacacct gctgttatac
    54241 catctttatc cttgaatatt gctgaagata atattacaac gtctggatta cattctatta
    54301 atggctccat attgtctgct cttagtccac catctattgc taattcacac ttaggattct
    54361 tttcgtctat catttttcta gctcttttta ctaaatcaac agcactcttt ctccatcccc
    54421 agttatcttt tccatcagtt tcatcaacac catgaacgac tatttgcaat ctatctatat
    54481 catatatagc ttcatcaaca aaacataatg gtgtgtaaca tccaattgtt aatcctactt
    54541 tcataccaaa ttctctacag taatttatta tgtatgcaag aggagcacct aaaaaatgtt
    54601 ctgctggtat tatcaacaag ttacatcctg ctgctgctat tttttctata aataatctat
    54661 cacaatctct tgtgtatata tggcattcta ttggtttatc tgtcactggt cttattgctt
    54721 ctattatttg atgaccaccc ataagttgca tattttttaa gtcatgcata tctgctgcat
    54781 ctgaatgcac gtagtctaca cctgcatcca ttgcttcttt aactacggct gatatatgac
    54841 cgtaatttac atgtgctaat cctgctgcta tttttatgtg tttcatattt gtatacctcc
    54901 atattttact ttaattagta ttaaatatta gtttttgata tttttttaat attttttaat
    54961 ccagtattac ttttatttag cattactttt attgctgtat atatttcttt tatgaactta
    55021 ttctgctttt gttaactgtg aacttatttt gctttgtaaa ttaatattac actgatatat
    55081 tttttttcac aacactttga ctactcctta aaagtacctt tatattgtgg taaattatag
    55141 acattaattg tatataaaaa gttagttttt taccacattc ttatggcatt tattgtctat
    55201 actaactcaa ttgatatagc tagttcattt aatatataat cagactataa atataaattt
    55261 actcgataaa aagtaccatt cataacaata ttaatgaaag ggagatgtaa aatgagaaac
    55321 tgtattattg cccaatctgg aggccctaca gctgttatca atgcttctgc agttggtatt
    55381 tatgaaaaaa atttagaaac taaatacttt gataaagtat attttggtat caatgggatt
    55441 gaaggtatat taaacaaaaa tataatagat tcagactcct taaacaaaga taatgtatta
    55501 aatttaaagc aaacaccttc gtctggtctt ggttcttgta gatacaaact ggctgattat
    55561 aaaataaatt cttatgatta tgatagagtt ataaatatac tagatgaata tgaaatagat
    55621 acattatttt atataggcgg aaatgattct atggacacag tgatgaaatt ttctcaatac
    55681 tgcaaaaaca ctacttgtaa aataaaattt ataggtattc ctaaaacaat agataatgat
    55741 ttattaggaa ctgacaactg tcctggattt ggttctgctg caaagcttat ttctacacta
    55801 ataatggaaa catacttaga tttttctata tacaaaaata atggtatatt tatagtagaa
    55861 actatgggta gagatactgg atggttagca gcttcttctt cactagctag acttaatgat
    55921 actccacttg ttgatttaat ataccttcca gaaattgatt tctatgaaga taagtttata
    55981 gaagatgtta gaaaaatatt taaagaaaaa aataaggctt atatagttgc gtcagaaggt
    56041 ataagggata aatacggcaa ctttatatgc gaacaaaaaa cttctgctca tgataaattt
    56101 ggtcattccc aattaggtgg agtaagcaac tatctaaaga atcttatagt atcatcatca
    56161 attacaacaa gagtaaaaac cttagaactt ggtgtaatac aaagatgtgc tatgcattta
    56221 gcttcagata ctgatttaaa acaagcagct gaagttggtg catatgggat agaactatct
    56281 aaaaaaggat atacaggagt tatggctatc attaatagaa catgcaataa tccttataag
    56341 tatgaagtta gtcatactga tatttctaaa atagctaaca agacaaaata ttttcctaaa
    56401 gagtggataa atgaagaatg taattatata actgatgaag ccctaaatta tctatatcca
    56461 cttattcaag gtaaaccaga tatcaaatat aaaaatggac taccttattt ttcaattatt
    56521 tactaggaaa ttattataat ttgatataca ctcaattcta tcgctcttaa atacctaagt
    56581 ggctgttaaa taaaattata aattgttata tgacagtcac ttaatcgttt aattatttaa
    56641 ctgtttagtt ttttaatttt ttaatctttt ttaatactaa tacgatttgt tgtaataata
    56701 tctcacaccc attttcattt tggatagatt ataattagtt agacaataaa tattttgtca
    56761 tgatattatt atatatagtg atacaaatag aaatactttt atcatggcta gaaaaacaaa
    56821 gattacacaa aggatttaaa aatcaaacaa cttcaattaa aataaatggt taaaataact
    56881 tctaaatcaa ctactataaa caataaaaga acataaagta gttaagtcta caattataaa
    56941 attagtaaga ctataataac ttaaataaaa ttaaagttaa caaagactat taaagttagc
    57001 aaatattcct ctacctaaat aattgctttt tataaagagt tatttatttt aattgaattt
    57061 aattgaaata cgattttaac tagaacaata gattaatgta atatttaata taaaataatt
    57121 ataataattg aaaaggaagt aatctatgat gaaaatagca gtcataacaa ccgaattttt
    57181 aaaagagttt gtagataatt ctataaaaaa gttaaatatt aatgcagaaa ttgaaatata
    57241 tatctataga gatttttctc atgttggaga tttatattta gaaatagaag ataggtttga
    57301 tggatttgca gttagtggcc ccatccccaa aaaagctatt acaaaaaagg ctggaactat
    57361 taaaaaacct ctagttgatt ttggtactga cttacaaagt tactatgata tgtttctaaa
    57421 gcttatcttt aaatataata cattagattt ttctagagcg tactttgact taacagactg
    57481 ggcagataat ccaaaagata tatcatatta tttatcaaat ggcacatttg gagacttaat
    57541 ggatagtatt gatgaaaatg catctaaaat gagtttatct gacatagcaa aacttgaaaa
    57601 aaagattgca aaaaaacata ttaatctatg gaatgaaggg aaaatcgatt tttctactac
    57661 tcgttttagt agtattgttc ctatgttaca aaatgcagga gtaaactttc atttcatata
    57721 ccctgatata gatttattaa aaaatacttt taataactta ataaaagata ttcatttaca
    57781 tagaatgaag ggaaatcaac caagtgtaat atatataaca gttaaagata caaattatga
    57841 tgactctact ctagaccaag tttataaaat attaaatagt attaaaaaag ataaattaac
    57901 cgattttata attgaaaaaa atagtaattc ttttaaaatt ttcactaaat gtgaaacgat
    57961 taaatcatta actaaaaatt ttactgcatg ttatataaaa gaatctattg aaaatagtac
    58021 aaatattaaa gtatgtgttg gatatggaat tggtaatgat ataaagcaag ctatgtctag
    58081 tgccataagt gctaataagg aatctaaaat aaatcattta aattacagtt tcttagtcaa
    58141 cgaaaattat gagacaatag gacctttact gtgtgataag ttacttactg tttcaaataa
    58201 agtaactcca catataaata caattgccaa aagttctaaa ttgtctactc ttactattca
    58261 aaagattata tctgttactg aaattctaca aagagatgaa gttactccta aggaattatc
    58321 ttcatgttta aatgtaacag ttagaactgc taatagattt ctaagtagtc tcttaaagtc
    58381 tggaaacgct actatattat atgaaaaaca aaactcaaca aaaggtagac ctgaaagagt
    58441 atacaaatta tttatatcag aaaataaata ttaaaatcca aaatgcataa aaatatttta
    58501 tcatctaata ttaagtaaaa ttatatatga ctttctaaaa ggtatatctt tgcatctttt
    58561 taagatatac cttttataat atttaataat attttatgtt attatctact aaaattattt
    58621 tctaaccatt ttactgcaca atgagcaaat aaagctgaac cataaactaa tccctcttca
    58681 ttaaaaattg ctttaggatt atgcatacca aaattatatc cctgactaat acttcccatt
    58741 ccaatccata aaaatactcc tggaaccctt agtaaaatct ctgaaaaatc ttctgaaccc
    58801 attgcagatg gaaagtctat tacattttca tttcctataa cttctttaac ataagataca
    58861 atctcatctc ctacatccaa atcatttata actgcaggag tccccatttt aaattccact
    58921 ttagcatttc ctccaaatga tttagctatt cctgtagata tttcaataat tctatttttt
    58981 aatttttctc taacagattg gtcaaatgtt cttaaagtac ctgacataga tgctttattt
    59041 ggtatagcat ttggtacgct tcctgcatta aacataccaa tagttaaaac tacaggctct
    59101 ttctgaggtt tttctcttga aagtatagtg tttaatgcta tttgtatatg tgacattatg
    59161 tttattgggt ccactccaaa ttcagggctt gctccatgag ttgcttttcc ttctactgtt
    59221 atttcaaata tatctgcaga tgccatagct ggaccacgtg aataagctac ttgacctgtg
    59281 ttaagaaatt tacccaccat actgtgaaat gccatagctg catcgacctg aggattttct
    59341 aatataccat catcaatcat tgattttgca cctctggtag tctcttcatc tggttcaaat
    59401 acaaacttta ctctaccttt aagtaagtgc ttattatcca taagtatttt agctgctcct
    59461 aagagcattg ctgtgtgatt atcgtgtcca caactatgag atgcatcttt attcttactt
    59521 ttaaaaggta aatccgtctc ctccataata ggaagtgcat ccatatctgc ccttaataat
    59581 aaggtctttc caccattttt actgtctaaa acagctacta ttccattatt agacgtcaat
    59641 ttcggagaat atcccatttt ttctaaagta ctggtaacaa aggttactgt tttcggtaga
    59701 ttgcaaccaa tttcgggatt ttcatgtaaa tatcttctcc atctaataat ttccccttct
    59761 attgactgcg cttctttaaa aaaatttttc atattccttc ctcctaatca gatagtcagc
    59821 aaaatctcac tttcttttag taatgaaatg atgctgatat aaaaattata attttatata
    59881 ttaaatttca tgtcttttta aggagttgga atatcatctg gaataggaca tttatatgtt
    59941 ttaccattca tagatttatc aaattcttct tttgcctttt tagctatctc tggattattt
    60001 aatattttag ctccaaacat agccataatc tttgatgcat atatcatacc tttttctcct
    60061 atagaacttc ctgcacaaga tgtaaactgc cagctatgac ctggtgctcc tagattcgta
    60121 caccctgtaa aaaagtatgc tgtcggaact atatgcatta catctccaat atcagtagag
    60181 ccataactat taaagtttgt aacatttcct ccaccataat atagatgagt tccttctggc
    60241 aaattatatt tacttatcat agccttatat tgttgtgggc tttgtctatc taattcagct
    60301 gcaaaatcca attcctctcg agtccaaggc tcttgtttaa tctcattcat acattctgat
    60361 acaagatttg ctaaaacatg attgtttaaa gtattataac accctcctaa aaactctact
    60421 tcaacttcag tttcagtcat cattgcagct ccttttgcta cctttacaag tctttcatat
    60481 gtactttcta cagcttctct acttaaagct cttgtataat accatactga tgctttatca
    60541 ggaactatgt tgggtgctac tcctccatct gtaattgtat aatgaatcct tacatcagaa
    60601 gtaacatgtt ctctcaaata attagctccc acattagtaa gttccacagc atctagtgca
    60661 cttcttccat tttgagggtc tgagcctgca tgtgcagtct ttcccttaaa gtgaaacttt
    60721 acagagttaa cagctgttga agtaccaatt gtagcttcac taattttatt tggatgaaaa
    60781 tttatagcta agtctaaccc ttcaaacgct ccacctctag ccataaatcc tttacctgtt
    60841 aattgttctt ctcctggaca tgcataaaat actatagtcc cacttaagtt attttctatc
    60901 atctctcttt taagccctac tactgcccct acatgtgctg tacataataa attatgccca
    60961 catccatgac catatgcacc caattcaaat gcttcttgtt ttgttgaaac tttttgattt
    61021 aaaccaggta atgcatcaaa ttctcccata aaacctataa ctggtttacc tgaaccaaaa
    61081 ctagctctaa tagccgtggc taaacctcct acacctactt ccacatcaaa tccttcactt
    61141 cttagaaagt ttgctgtatt ttcacatgct ttaaattctt taaatgcttt ttctggattt
    61201 tcccatattt tcttacttaa atcatcaagt ttgaatcttt ttttatctat ctcatttata
    61261 gatatacttc ctatcatttt ctattcctcc tataaatcta tttactatca accaaatata
    61321 attggaccta ttatactagc aaatgccact gatgcaatgg ttacagtaac aaatcctcct
    61381 ataatcatct ttggtaataa ataatctgtt gccctttgtt tttcttcttc agttccttcc
    61441 atagcatcaa taccttcatt ggaaattatc tgtgttgcag gataagctag catacaagtt
    61501 acaccaattg cagctgacat tcttggagaa tatcccaata gttttcctac aattgcagta
    61561 aatagtatta taaatacaga gcctacaaca agtattccta tagttggaat aatcatccca
    61621 aacaatgctg ttggagttac cttagcaagt cctccaggta acatcaacat acaaccaaac
    61681 attataattc cattagattg agatttatct aaaattcctt tttctaaaaa acctattcgt
    61741 gtaaaaatca accctactaa caaacatgct atgttgggat ttaaataata gtttgcaggg
    61801 gtaaatcctg gtattaaagt agccaatcct acaaaattac caatcatagc aacaaatgaa
    61861 agctttgcaa gatatactaa agtagatgat tttttaccta aatcttcttt aaaaattctc
    61921 atacttggaa gtttaaaatt actttttgac tcatctttca aaaaatcccc gttatctctt
    61981 ttaaatttta attctttatt tatacaatat gtagttatcg gaatgccaaa gaacttttga
    62041 aatgataata ctagaattac aaatgcagca agttcaggtc tatttgcagc ttctgcaaca
    62101 gttgttacga taataccagc aacagtagaa cccgctattg gaggtgctgc tattaaagca
    62161 tactctctac caaataacat actacccact gttaaacagc caacccctat tcctacaata
    62221 cctactaatg caattacaac cgttttccat tcatgacata agtcttcaag atttataagt
    62281 gttcctaagt tagttattaa taaagctata ccaaagttgc tcattgcagc taatatccct
    62341 gatgctcctg taatgtcttt tggtaaaata tttgtccaaa atcctagtaa aaatataata
    62401 catacaaata acaatgttga aattgcacct tttgttttct tagataaaaa ctcactaaaa
    62461 gcataaatca ttacacatac agtaaagcaa gtaagtggat tccaaaaatt ttccattgtt
    62521 gtttcctcct aaatataata attttttatc aataaaacaa taattataaa atgtatatta
    62581 tgcagttatt cttaactaca tatggtatag cttgatattt tttgatgata ttatatacgt
    62641 cctaaattat tcctaaaata tctaaataaa tataactaga gttaactctc taaatattaa
    62701 ctatagttat caacttacta gatttctttt tgatttttac tttctacata attttacgag
    62761 ttagaattgt acttcaacta ttctctatat tagtatacat gttggaagac ttatat
//
LOCUS       DAEPIN010000013        62050 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.013, whole genome shotgun sequence.
ACCESSION   DAEPIN010000013 DAEPIN010000000
VERSION     DAEPIN010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 62050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62050
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.013"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            complement(1036..2592)
                     /locus_tag="KR250_001009"
                     /pseudo
     CDS             complement(1036..2592)
                     /locus_tag="KR250_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860844.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein CwpV"
     gene            complement(3376..3564)
                     /locus_tag="KR250_001010"
     CDS             complement(3376..3564)
                     /locus_tag="KR250_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0066974.1"
                     /translation="MNNKKKAFLISGAINVISLIIVFSLFHYNIIENSTQNMIITVIL
                     IFIFEMIKIKIIEKYYKL"
     gene            complement(3590..4729)
                     /locus_tag="KR250_001011"
     CDS             complement(3590..4729)
                     /locus_tag="KR250_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HBH0066975.1"
                     /translation="MVIKLTIEEYKNKYFTGNKIVLSINSFDNKELYYHETENILNLI
                     NEIDKLTSSKIFKTIEDKNDLALESYYSSVIEGAFSTRKIAKSIIRGKMKPSNKSEYM
                     IYNNHRALEYGLDNLDKLYSHKFIYDLHHILGENCLDSEEYEYRTEKVYVCDSKGEII
                     HTGLEPLKIYDFMSKLIDFMENSKVSNLIKSAIIHFYFVYVHPFSDGNGRTSRALSYL
                     YLIDKGYDTFKDFSISYMISKNRTKYYKAILDVENKGNNLTVFIEFMLKSIIQSINEM
                     RNMHDRKSLESILKEELFENDITLSATEEHILKYICNKDNYSMTLENYIKKNKSRYLK
                     AGIKEIELVDQLMEVFNNLEEIEILSKEKNIYKVNDKYLKMLDID"
     gene            complement(5266..6105)
                     /locus_tag="KR250_001012"
     CDS             complement(5266..6105)
                     /locus_tag="KR250_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBH0066976.1"
                     /translation="MSDIKNEVSKKTLSKKDVIKSWLRWFFFAQSNYNYERLQSTAFS
                     HSMLPVLKKLYPDKEELKQEVETHLAFFNTEPICGCVIHGITIAMEEEKANGADEISG
                     DGMNAIKTGLMGPLAGIGDTLTQGVITPIVLAVCIGLTEGGASIAGPILFVIAQYIIM
                     TSISFGMWTNGYKYGKKAVESILQGGIVNKVIEGASILGTLVMGGLVGRFINLSTPIS
                     YTSGDFKFSLQTDLLDKIFPGLIPLVLTLLVLFALKKGLSPIKIMFILIVVGAITGIL
                     GLF"
     gene            complement(6129..6887)
                     /locus_tag="KR250_001013"
     CDS             complement(6129..6887)
                     /locus_tag="KR250_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBH0066977.1"
                     /translation="MHISLIQAVLIAIFYYLSWSPWLTYVGFFTWNRPLLAGFVTGII
                     LGDPVQGAIIGAGINMIYLGFISAGGAQMGDPAFAGYVGTALAIASKLDVSTAMAIAV
                     PLGTVATVLWIGKMTVNSFFAHWADREVQKGNVDRVAFINIVPPQILLFAMSFIPALL
                     VVYFGPNAIDGMLEVMNDNVLHVFNVIGAMLPALGIAMNLKLIGNKFTMPFFILGILM
                     AVYFKVDIIVISVIGVILALTITSIKYGKDSATS"
     gene            complement(6934..7407)
                     /locus_tag="KR250_001014"
     CDS             complement(6934..7407)
                     /locus_tag="KR250_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBH0066978.1"
                     /translation="MSNIALTRIDDRLIHGQVITAWCKITSAKRIVIVDDLVVKDPFI
                     VQVLQMAAPSTVKVEVHDVESGAEVLKSYNGDENLIVLVKYPKTVLGLVNSGVDLKEL
                     NVGGMGAGLGRKSFYKNISVSDEEKEIFRELISKDVKCFIQIVPDAKKIDVGTLL"
     gene            complement(7436..7852)
                     /locus_tag="KR250_001015"
     CDS             complement(7436..7852)
                     /locus_tag="KR250_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBH0066979.1"
                     /translation="MIHMAGIIIATHGNMAKAMLESAELIVGKQENVETLGLNHGDSI
                     DEFSRKLEDGIEKYKDEGVLVLLDFYGGTPFNTSAIIINKFAKVCELECLTGVNLPML
                     LELFLNRDTMKLKDLKNLCEEVGISGIKDVKTVLKL"
     gene            complement(7899..8951)
                     /locus_tag="KR250_001016"
     CDS             complement(7899..8951)
                     /locus_tag="KR250_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBH0066980.1"
                     /translation="MKSVRFYGIRDTRVEDVDVPKILEKDDVIIKVKVAGICGSDISK
                     YSKTGPHMVGEILGHEFSGEVAQVGKEVRSFKIGDRVAVCPAMPCFECDECKKGLYSR
                     CNNVAIIGNKELGGCFAEYTKVKERNLIKIPDEISYETAAALEPVCIAGHGLFRSEAK
                     VGDTVVVLGTGPIGLFSIQWAKIFGSTKIIAVDVFDEKLDLAKELGADICINAKEKNI
                     VEEIKRLTDGDGADIVIESAGTPLTCGQVLLLAKKGGTVLYAGVPYGDVALTREQFEK
                     IVRSELTVKGTWFGNSFPFPGKEWSAGLYHMQKGDMNVEKLVTHRINLEEVPAYFEKV
                     YKRDIFFGKIMINIDN"
     gene            9347..10027
                     /locus_tag="KR250_001017"
     CDS             9347..10027
                     /locus_tag="KR250_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HBH0066981.1"
                     /translation="MKKVYQGKTKDVFELDNGNYLLKFKDDVTGKDGVFDPGENSVGL
                     SIDGIGRANLETSVKFFEILNNAGIKTHYVSANLEEATMEVLPAKVFGNGLEVICRYR
                     AVGSFLRRYGSCVEEGAKLDCYVEATLKDDDRCDPLITSEGLEALNIMTQAQFDSMKS
                     MTQKISNIVRDTIAEKGLELYDIKFEFGFYNDEVILIDEIASGNMRVYKDGVIVDPMD
                     LTALLLDK"
     gene            complement(10160..10480)
                     /gene="sugE"
                     /locus_tag="KR250_001018"
     CDS             complement(10160..10480)
                     /gene="sugE"
                     /locus_tag="KR250_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000118520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HBH0066982.1"
                     /translation="MKWLFLFVAGLFEVFWAFQLKNSHGFSKLTPSILTVVGMIVSFY
                     FLSLALKHLPLGTSYAIWTGIGTVGTAIVGMAILNEPISISRIACIGFIVVGIVGLKL
                     LSTN"
     gene            10862..11689
                     /locus_tag="KR250_001019"
     CDS             10862..11689
                     /locus_tag="KR250_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HBH0066983.1"
                     /translation="MNFYKIAVCQMITTENKIENINHAVDMVTEAAINGSKIVVLPEM
                     FNCPYENKYFPKFAEEYPGETTTILSKLAEKHGIYLVSGSIPELEDGKIYNTCYVFDK
                     NGALIGKHRKMHLFDIEVTGKVSFKESDTLTAGNDVTVIDTEYGKVGIAICYDIRFPE
                     LSRLMALKGAEIVILPAAFNMTTGPAHWELSIRMRALDNQIFYVGAAPARNMNASYIA
                     FGNSRISDPWGRIIAQADEKECIIYADIDRDLIPDIRQQLPLLKHRRTDLYELNTLK"
     gene            12511..13185
                     /locus_tag="KR250_001020"
     CDS             12511..13185
                     /locus_tag="KR250_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0066984.1"
                     /translation="MNNKILVVDDEEGIITLLKDYFEMNGYEVYTAQSGKGALKKISK
                     NPDIILLDINMPEIDGIEVCNRIRDYVSCPILFLTARIENTDKINGFRAGADDYIVKP
                     FDLDELGARVSAHLRREKRKSNQTKIKFYNDLTIDYEKRLICIDNNQISLSKKEFDIV
                     ELLSMNPGQVFDKEKIYDRVWGYNSNGNSDVIMEHIRKIRIKLSKHTLENYIETVWGV
                     GYKWIK"
     gene            13173..14333
                     /locus_tag="KR250_001021"
     CDS             13173..14333
                     /locus_tag="KR250_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBH0066985.1"
                     /translation="MDKINNMSLKKAFFVLTLSSLIIASTLTTFSYILLNNLYNSIQD
                     KYLKTPINTGFITIVQNNVNQFSDYDKTLLNIINILQLVLPLIFFIGLLLLADIIFYK
                     VKLKTPIEILNKGAMEISNNNLDFCLEYNNNDELGNLCNAFEKMRSELNKNNIKMWTM
                     INDRKQLNAAFSHDLRNPLTVLKGYSNYLTKYIPTGKLSDEKILSTTQLMSEHINRIE
                     YYVENMSNAQRLEDLVVSKSMSNINKFIENLDENISIIAKQEGKSFTLKSQINNINLF
                     FDENIILRVVENIISNAFRYARNNVSILIYLEQELLTFVIEDDGIGFSKESLKLALRP
                     FYRDKTLNDSNAHFGMGLYISKILCEKHGGSISIENNSTNSAKITAKFSTRN"
     gene            14604..15077
                     /locus_tag="KR250_001022"
     CDS             14604..15077
                     /locus_tag="KR250_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HBH0066986.1"
                     /translation="MLSFTFCSLPFWICYFWCGISDIFDGLIARKLRQQSGIGAKLDS
                     IADFIFSVTILVTVGKNIALPMYLWWCIVAIALLRLISYSIGFFKYRTFSSLHTLMNK
                     ITGISIFAFPLLYICFGLDVAGIMICIVAFLSSFEEIAIMIKSKELNRDIKSIFM"
     gene            15363..16031
                     /locus_tag="KR250_001023"
     CDS             15363..16031
                     /locus_tag="KR250_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0066987.1"
                     /translation="MIIKAKQLSKIYGSNNNKVIALNNVNLEINSGEFVSVIGPSGSG
                     KSTLLHILSGLDNPTSGQVLLDDKDIYKHTEKELSALRRKSFGFVFQQFNLLPVLTAS
                     ENISMPVLLDKKQPDKEYLNEISSLLGIADRLNHLPHELSGGQQQRVAIARALIAKPD
                     IIFADEPTGNLDSKSGSEVMNLLIKTSKQFGKTLVVITHDDRIAKLADRKISIIDGVL
                     MEVK"
     gene            16034..18502
                     /locus_tag="KR250_001024"
     CDS             16034..18502
                     /locus_tag="KR250_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0066988.1"
                     /translation="MKNYLSLSIKELKSQKLMTTFIIIAIVLSTIMTTVVGQSIGILQ
                     NLRIEQARSFNGDRHVSFHQLTKNQVDDIKKDDRVYQAGTSITLGSSKIKDSGISVLV
                     KEYDKTGLSNYPKLMKLKSGNLPKDKNEIALDENTLKLMGIKPRLGVTIPMNLDISLL
                     NDTIPPYNYTANFKLSGILEDDYTGYVSGIVNGIVGKGASENLLPKRYILSSLDFKIK
                     QQEKFQEIVNQLAKKINLSHNSIQYNWIYLNALKIQFEKDENSSNSDGISMIILVSLF
                     VALLVLLASGLVIYNILKISVTKKIKEYGCLRAIGAEPNQIYKIVILQILILCTIAIP
                     IGAVIGIISSKGITGMVTNILNPDILLANDNKEITELIHKNTTAYIFPLVLSTSISLI
                     FSLISALPSAIYASHVSPKIAMAGSTTKIKRKIKREKTIKNFERHLAWLNLKRNKGRT
                     IITILSLFMSITVFVALSEFSNVLDVSRSVSNLKEGDFSLTNEISGFDKSILDKIDKM
                     KNVNRTSFIKYSEYKQGEIDTDINFENSGEMLKIIGIDEQTLKDLMPSITDSILEDFK
                     NGSICFIKNPIAISTPGVKTRHTNLKPKDNITINKKQLDIYSTVDKMFFLQGNGWING
                     VDVIVYDSVYNTLTNKNKFNQINIYAKDKSKLEQIRLSIEQICENNPGSHWISYIESD
                     KQLKESFKQIEFLAWAVILFVGLIGTLNIINTTHTNINTRTNEIGVKRAIGMSNSSLY
                     KMFLWEGVYYGIFATIFGSIAGYVSAIIINMATIEKLDFTNIPITSILQATIISILAC
                     IIATLIPLRKVKKMNIIDCIDVNL"
     gene            complement(18741..20165)
                     /locus_tag="KR250_001025"
     CDS             complement(18741..20165)
                     /locus_tag="KR250_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBH0066989.1"
                     /translation="MKNQSLITQFRHTFIFIIIASIVATVITYVFALYLYIHSLNKDI
                     YPPNYYERQVPRIEKYINEKNITLLSQSNEEGLKRTIRGDDMLYQVVDNNGNILYGTN
                     PKKLFKTKEELFNNFINKTVRKGGYIHTVPIKGDNGKIEGAVILFYQVKITFANIRGR
                     FVFAVIIMALFSPFLYIVGFTRWLSKRFVKNINQPLHLLIDASKKIKEKDLDFEIDYY
                     SDNELGKLCSAFSEMKDELKGSLSAQWKMEQERVEMVEALAHDLKSPLSIILGYTDAL
                     IGNNTDDNEKLHRYLTVIRENTEKSAALVQKMQYTSDLEKSNIQLNLVPINLPEFLRQ
                     KVQDYELQAHQKEVELILKMQGNIQSPIQIDVDRLTRIFDNIISNSLQYTPSGGNISI
                     TVKDEKNCISYEICDSGRGFSSKDLKKALDKFYRGDEARQTKGGHSGLGLYIVKQLVE
                     QLGGSVKIENSKSGGACVKFWHSI"
     gene            complement(20184..20876)
                     /locus_tag="KR250_001026"
     CDS             complement(20184..20876)
                     /locus_tag="KR250_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0066990.1"
                     /translation="MSKILIIDDEMDLVMLLEDELKAKGHEVLIAYDGQAGIELSKQE
                     PDLIILDIMMPKMNGFEVCQAIRDDVLCPIIFLSAKQSETDKIKGLTFGGDDYVTKPF
                     GLRELMARIEANLRREKRSQYINEENKRSTLYFGKLCLYMKERAIKIDGKNIDLTKIE
                     YDIVELLALHAGQVFSREQIYEKVWGCDSEGDSSTVVERVKKIRAKFSAVTPEKEYIS
                     TVWGIGYKWNKM"
     gene            complement(20931..21707)
                     /locus_tag="KR250_001027"
     CDS             complement(20931..21707)
                     /locus_tag="KR250_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutG family
                     permease subunit"
                     /protein_id="HBH0066991.1"
                     /translation="MIKLLSSEWLRAKRTAVQWLTLFMPIIFSLCVVFYLTTKSGSTQ
                     EFAFEGFFTVWTVFIIPIGVGILAGFIVQEEELAGNFNGFLCVGISRVRLYLGKFLFL
                     LFCLTICTFIATLILCIGMSIAVPSGATIWLFLSAAGLVVIGTLPLLAIHLWVSFAWG
                     MGASIGISIGGILMAAILGLTSVGEKVWAFVPWTWPVKLGMLPGIYFIKEAGTISTEA
                     FYSKVMQTASIELIVVAIGLIIFLIGGVIWFKMWEGRKSY"
     gene            complement(21704..22474)
                     /locus_tag="KR250_001028"
     CDS             complement(21704..22474)
                     /locus_tag="KR250_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HBH0066992.1"
                     /translation="MNALQSELLKYKRTFMGKLIVFFPVSFAAYAFIMQSTLMQNPLS
                     QTTSWAWQSLLALIFNWWSFLFLPIGFALFATLVAFQEKKAGNYRALRTHNVSPMTLW
                     INKVIAMAVYSFISTLVLIVVTIITGLILKAGPVPFGQIIGASIVCWVVSLAILPLQL
                     WLATWKGMFLSMGVGALGMIFGVLAATKPFWIAVPWSWAVRMVCPIINIHPNGTILEA
                     GDPLLNTSVIPIGIVVSLVVFIVLTALTAAWFNRRDDK"
     gene            complement(22471..23175)
                     /locus_tag="KR250_001029"
     CDS             complement(22471..23175)
                     /locus_tag="KR250_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic protection ABC transporter
                     ATP-binding protein"
                     /protein_id="HBH0066993.1"
                     /translation="MKDLILETKNLSKRYGEQMAVNNVSLQIERNSIYGLLGPNGAGK
                     STTLKMLVGLLRPTNGRIIFDRKPWKRESLAKIGSLIESPALYGNLTAEENLLVHTKI
                     LGIPKDKIYEVLEIVNLKNTGKKRASQFSMGMKQRLGIAIALLSDPELLILDEPTNGL
                     DPFGIQELRELIASFPKKGITVILSSHILSEVAQVVDNIGIINGGRLLFQGMPDPNEN
                     LEEFFTDVILKGGQNR"
     gene            complement(23430..23942)
                     /locus_tag="KR250_001030"
     CDS             complement(23430..23942)
                     /locus_tag="KR250_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0066994.1"
                     /translation="MKKISQEKISTHIQEVPQEKIEQWKSLYEKYKSILKVNSKSVLE
                     IIEFLKRKYVIVEETSEEFKHVVINNIKLNKPFSQKISSDKELKPIVFTIPNEGNAKK
                     LYDTQEEIYSGCKIIVGMEFETGYVFVEGSDELNDEITSFKGLDKYDINNYYLVANYI
                     RCLKKYKLID"
     gene            24268..24525
                     /locus_tag="KR250_001031"
     CDS             24268..24525
                     /locus_tag="KR250_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0066995.1"
                     /translation="MILNRAELFRGYSLNRFNEICNILSVNKIKYKYKVKNNAYSKKP
                     FFNEITLGNLGQKEDFSYEYLIYVHKDDYEQAESLINSKLR"
     gene            24668..25294
                     /locus_tag="KR250_001032"
     CDS             24668..25294
                     /locus_tag="KR250_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HBH0066996.1"
                     /translation="MKINIKSKLSEFIKQNNLFDDSRIISYLPNITIETDKIKTIMIN
                     EVVPTNTNDDFYSVDKDADYLKTTIPLFDSAGIKVSNINDILDMGIYITNAVKLPKSE
                     YTITRDTIKLHMPILEEEIKLFKNLEVVMLMGDVAKKSFNMITKKHIKKNVIPSISTY
                     KLRNNELYYDNLRVFPSYIMTGGNILIEKSKFEMASEDIKKMFEIING"
     gene            complement(25541..26320)
                     /locus_tag="KR250_001033"
     CDS             complement(25541..26320)
                     /locus_tag="KR250_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901940.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HBH0066997.1"
                     /translation="MNSKIYYFSGTGNSLVVSNILKETLNGSKEVIPLAIHRKENNID
                     INEDILVIVFPVYFADVPDIVKSFVEKLNFNINTHIYAIATCNGVAGHSLFTIDKLLK
                     KRGKKLSAGFLINMPGNALITPPDVEEERLKNQTNRVAEIAKCINNLEIGNIEGKNNL
                     KSHIDSVVLRLVGKNMQIATTKFFAENKCNRCGTCKRVCPVENISIVDKKPKWGNECE
                     RCLACFHWCPKEAINVKKSFLTNRKKYHHPDITVKDIFLDK"
     gene            complement(26388..26849)
                     /locus_tag="KR250_001034"
     CDS             complement(26388..26849)
                     /locus_tag="KR250_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBH0066998.1"
                     /translation="MGGARDYLNGGKMKDFEKLYEATTKLVSTSLKVKDDLKKMFKQN
                     GYDITTDHYALLRFLWEQDGISQIDLCEKSCKDKSNTTRILDVMKNKGLIVRKVDVKD
                     RRKFQIFLTDLGRELEEPLNEIASIYATETFKSISDEELPLFTDILDRIGK"
     gene            complement(26825..27502)
                     /locus_tag="KR250_001035"
     CDS             complement(26825..27502)
                     /locus_tag="KR250_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBH0066999.1"
                     /translation="MIITVINGSPRKNGATSKVLTYLYKDIERLIPDVKINYFDLSEV
                     NPSYCIGCLNCYKMGKCINQNDKVEYIHDIITKSDGVIFGSPTYGSSVTGLFKVFTDR
                     AHMMLERLLYRKPCIAVTTYENARGSKAISFIKSMVLDSGGYVCGSLSIKTGFNQNPI
                     TEKVESKIQKVSKKFIYCIEEKKNPPVLSQIYNFIAINAVLKPMAFKDIEQYKGIIDR
                     WEEQGII"
     gene            27735..28121
                     /locus_tag="KR250_001036"
     CDS             27735..28121
                     /locus_tag="KR250_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBH0067000.1"
                     /translation="MKKLPHISEAEYQVMKIIWKYAPISTTEVIEKLVETSTWSPKTI
                     QTMLLRLVKKGALTYEKNSRVFVYTPLVKEEEYVATESSSFLNRFYNGTLNSMVLNFL
                     ENDKLSEDDIEELREILNKRTTKGDK"
     gene            28124..29923
                     /locus_tag="KR250_001037"
     CDS             28124..29923
                     /locus_tag="KR250_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaR1 family beta-lactam sensor/signal
                     transducer"
                     /protein_id="HBH0067001.1"
                     /translation="MYAPFFIRFLLSTLTLSILIVVILLAKKVFKKHISAKYQYNIGF
                     ILLIMLIIPFVPLKYINLGNVFNYLYTFNQLGSSTNRTSMIKNSSNSVINNSNALNDF
                     SVSLNQVNFDSLNILLLISWIIGILIMIAITIHCNIKIKNMKKSIQILKDEEIVFIFE
                     KCKKDIGINKDFILCKSPIINTPITFGYFKPHIILPDKSISKLSLKDIKYILLHELQH
                     FKNKDILINYIMCFLQILYWFNPLVWYAFKEMRIDREIACDISVLKKLNKDSHIEYGQ
                     TLINFVDNISRPSNLTLTSDIGGSKKQIKKRLLKIMDFHNESNTLKFKSILIFTLIAL
                     IVFGNIPKLSIMADINEKYRPTNEQTVYEDLSQYFKGFDGSFVMYDLKSKQYHIYNKE
                     KSKTRISPNSTYKIYSALLGLENGIITRNNSHIPWDGKHHSNSSWNEDQDLFSAMENS
                     VNWYFQTLDKKTGLKNIQTYLRQIGYGNYDVSGGISNFWLESSLKISPIEQVELLESF
                     YTNKFKFEDKNIETVKDSLLISKKDGVSLFGKTGTANINGKDVNGWFIGYVEKNENTY
                     FFATNIQSKDYSNGSSASKITLSILNDKKIYNP"
     gene            complement(30278..32152)
                     /locus_tag="KR250_001038"
     CDS             complement(30278..32152)
                     /locus_tag="KR250_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBH0067002.1"
                     /translation="MNKYNKIANELIKIIGEDNIISITHCATRLRVMVKDREIINDKK
                     VEKVDEVKGVFFTSGQYQIILGTGIVNKVYAEVEKMGLKTLSKKEQDELVKNNETGFK
                     KVMRTLADIFVPIIPVIAATGLFLGLKGCLFNDNVLGLFGMSSANIPLYIQTLVSVLT
                     ETAFAFLPAIIVWSAFKVFGGTPVIGLVIGLMLVSPILPNAYSVADPSNEIEAIMAFG
                     FIPIVGCQGSVLTAIVTAFIGANLEKWFRKHMPNVLDLIFTPFFVMLITMLVILLGVG
                     PIMHTIELKMVDIISLLIDLPLGIGGFIIGFTYPLAVITGLHHTYVMIETSLLANTGF
                     NALITLCAMYGFANIGTCLAFMKKSKNNQVKQTAVGAMLSQLFGISEPVLFGIQLRYN
                     LKPLIIMCASSGLGAAILSILHIQSNSYGLAVLPSYLMYIYDGYNLITYLLVSIFVVA
                     FCFIVTCLFGVPKEAINEDEDEELVFNENNENFVSPAKGKIVALENVPDETFSKKMLG
                     DGFAIDIIDGKIVSPISGKLETVVSSGHAFGIKGTNGEVLIHVGIDTVALNGDGFDVA
                     VKQGDMVKQGDVLVNVDLKRIHELGKSTLTMVLFPDGKKVNILDINKDVKIGQRICVE
                     "
     gene            complement(32157..33821)
                     /locus_tag="KR250_001039"
     CDS             complement(32157..33821)
                     /locus_tag="KR250_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HBH0067003.1"
                     /translation="MEKKWWQKEIVYQIYPRSFQDSNNDGIGDLQGIISRLDYLKNLG
                     ITSLWLCPIFKSPMDDNGYDISDYLDIASEFGSMEDLKELIKESKDRGIKIILDLVLN
                     HTSDEHPWFQEALKNPESPYHDYYIFKKGNDLPNNWRSVFGGSVWEKVENRDEYYFHS
                     FGKKQPDLNWENPILRSELYQMINTWSQMGVAGFRVDAITFIKKNLSFQSLESDGVDG
                     LVKCTKTVRNQPGIEKMLYELKENTFKKYDCMTVAEAAGISYDQLGDFIGEDGFFSMV
                     FDFKHADLDVASGSEWFKRISWTIPDLRKCILDSQMYVQEHGWAANFIENHDQPRATT
                     KYLLQYERNDDAVKMLGAMYFFLRGTPFIYQGQELGMTNFKRDTINDFNDLSSIDQYY
                     RSIDESYSEEEALEFVNLRSRDNARTPFPWADKENAGFSDTTPWLKVIENYKDINAQS
                     QINNSESILEFYKKMIKLRQDSEYSDCLIYGSIEPIDIESDDIIAYRRQLDLVVIDCY
                     YNFSDKPVEIRVEEKYDIVFQNLDDINLLNKTVRLKPFHAVLLKVR"
     gene            complement(33835..34656)
                     /locus_tag="KR250_001040"
     CDS             complement(33835..34656)
                     /locus_tag="KR250_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBH0067004.1"
                     /translation="MKLYKLLILDIDGTLRDEVYGIPESAKHAIRLCQKNHCSVVICT
                     GRSMGTIQDDVLSLGVDGYIAGGGNYIQYHGELLYNQSFNQRLIKEVVCLLKKREVAF
                     SIESQEKVFMNQKAKEIFETMNQLKGTNSCINKQHIQEKITYENNIEEYKSQDIHKIC
                     LWSNEKVFDEVKNILQDKMELAQRDISSQYYEIIQKDFHKGKAIKRLQERLGVTQKET
                     ICFGDGQNDVVMFQASDVTIAMKNSHQQLKDIATSICEDIFDNGIYKELKRRNII"
     gene            complement(34755..35519)
                     /locus_tag="KR250_001041"
     CDS             complement(34755..35519)
                     /locus_tag="KR250_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBH0067005.1"
                     /translation="MKIEGLFNKYYNSFTANDKYICECILNHKEDCIKLSIDEFADKY
                     HISTSALSRFAQKLQLPGYSELRVIIRLNETEVEYKNQSKHQLMDCYNKVIDDIEKKD
                     CSIMFERMKKANRIIVFGEGYSQERVAKEMKRIFLPTGKIIYDVYGYDMIQALNHFIK
                     TDDMIFFISLNGDASTIIDFAKEMRMKGIYMVSITKMISNPLARLCDENLYIQSISIS
                     VDKQLDYDVTTPYFILIELLYIKYKMYLESEHIPRK"
     gene            complement(35516..36265)
                     /locus_tag="KR250_001042"
     CDS             complement(35516..36265)
                     /locus_tag="KR250_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBH0067006.1"
                     /translation="MRLESLVNQNYDNLNENDKIIIKQILICQREYRNFSCEKIANEC
                     NVSRATLLRLCRKIGLDSFSDLKYLLKNNQLEFVNNDNNFHDVCENYRLLMNELQKIS
                     FQDICKIIYDAETIYIYGTGNEQKSLAQEIKRIFLSVGKCVIDLFDIGEIEFMRESFQ
                     KNDLFIIISLSGETKAGIEIAKFINNHIHTISLTRLDNNTIATLCEYNLYVETQKLDT
                     LQAISYEVVGAFYALLDLLHMNYIEYRRRKE"
     gene            complement(36769..37374)
                     /locus_tag="KR250_001043"
     CDS             complement(36769..37374)
                     /locus_tag="KR250_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBH0067007.1"
                     /translation="MKIKENVLMLDSTRGSNCFIVFDKEIILIDTGLPFMGKNIIKEL
                     KTLGIKLTDIKHILLTHHDVDHISNIKLLKEKTGAKVWAHVEDIPFIIGEKDRPGFKK
                     FIGKAISRRLIKDVEPYGENMQIGNIKIIHTPGHTPGHVCMIFEAVLFAGDLVKNKNG
                     NIVPYPNPWNWDYKKMVKSIRELDNLKYEWICMSHGTPCIK"
     gene            37553..38191
                     /locus_tag="KR250_001044"
     CDS             37553..38191
                     /locus_tag="KR250_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBH0067008.1"
                     /translation="MSELSKRDKEKIQRENEIIDKAEKLFCLNGFDNTTMNELAKEVE
                     YTKRTIYKYFSCKEDLFFAVVLRGYKRLWDNVKIESAKGKTGFEKIKLSYFAFHKFYS
                     VEPSLLCLMGMIGIVNSEKSDTDMLFKEKFFSFNKFMFDEIQGMFEIGKNDRSIRHDI
                     EIPTLMYSSIFTLTGFFNLLSVTGKSYLNNFNIDEEKFIETTLALLIDSIKA"
     gene            38323..38985
                     /locus_tag="KR250_001045"
     CDS             38323..38985
                     /locus_tag="KR250_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0067009.1"
                     /translation="MAKILLLEDDDTITFGIKTSLEKKNHTVCCYNTIAEAKKNFDKT
                     IDLILLDLNLPDGIGYEFCQYVKSIEDTPVIFLTVRDDEKDIIRGLDMGADDYVVKPF
                     LLSVLQSRINAVLRRTHSNTNNTITCENISLNKVEVKAYLDNVEVPLTAGEYKLLLSL
                     MENKNRAMTRTKLLEMLWDVDGNFVNDNTLTVTIKRLREKLKNPPCIKTLRGIGYRME
                     DS"
     gene            38985..40166
                     /locus_tag="KR250_001046"
     CDS             38985..40166
                     /locus_tag="KR250_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBH0067010.1"
                     /translation="MKTPRNINSTVVFIIFCLIVTFLLSVILTTITSFNEYNVLSKAL
                     GASYEYNPKSTESILKSLKNSSDKDFKTGNEILSKHGYSKSTFWSKNFFYFLVISFGI
                     ISTMATVIYIIKHRTNKQKLSRINNLTQYLEEVNLGKESVLLRCEDEFSHLEDEIYKT
                     VGELRCSKEFALKERQTLSDNLADIAHQLKTPITSMSLMAQLLSENCPDEEIDYINRL
                     TNQISRLERLVSSLLTLSKLDASTLIFESKLIDVHSLLTYAIEPIEGSLREKHQTFTI
                     EASPSVEFEIDINWTLEALLNILKNCSEHIEDGGYIVAYYSQNPLYVEIIIEDNGKGF
                     AQEELPYIFNRFYRGKNASKDSIGIGLALSKSIIERQNGTLHAENCHNGGARFIIKFY
                     T"
     gene            complement(40347..43007)
                     /locus_tag="KR250_001047"
     CDS             complement(40347..43007)
                     /locus_tag="KR250_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0067011.1"
                     /translation="MNILQEYTLDFVRRNKRSSIAIMLAILLTTTMMSSLCGLLYTMW
                     TDFIRLSIEKEGNWHGELFDYTYGSDLPLIENFSSVDDVMVKGQWYVGKINDEKRDYI
                     IYRNATSEYWNSMPEKDTIIEGRRPNNANEIALSKQYFENNPSVKIGDKITLPIGYRI
                     LNGKPLEPIAPKSKGESFKKISEATLTIVGKVDVSTSSVIPAYTALGYLDKTSIKPND
                     SITVYLRFKNIRDTYKELPKLAKSLGWKTNEYGKYNLKYNSNYLLKMLVFSPEQKASM
                     SSISKFSTPIMYLTIAIFTVAVFVMVIYNAFSLSANARLTQLGILSSVGASPKQIKRS
                     VVFEGFLLTIIPLPIGLFLGWLLCNRLIVYVNSVNYHTDVPEVVFTYGIPAFLPAVLL
                     TIVTVWISALIPARKVSKISPIEAIRQGDSVKIKKSHKYSLGKIFGIEGELASNALRA
                     RKKSYRTATISLTLSFLLLTCFLHIIANQEGAKAVYGSDNYKDQRDIAVYLENSEPIE
                     SAFIEKLDNTEGVKSSLYYQKMSASTWLTEKDASKELAKNGGFKSIVSSKKYSPIERD
                     GKFRIISNIIGLDDKSFTEYCRQISVDPKLFYNTKNPTSIVYNKEEDINRSTRRNKVY
                     IDYLNLSIGDNIKLNEKVYDEDKGDYTYNMKVGAITSTLPKIVDTSSKYTLMQIVPMS
                     MVQEISKNYDEVSRLRSNRLIGVFKVNNREDIPRVREKLESIYNKDYGSGDYSIEDVL
                     ESEKQKASGIKTMNLIVGFITGLLAMIGLSNVLATVSGNIRSRRQEFAMLRSVGLSPE
                     GIKKMLILEGLFLGITPLLLSIPVQIGIVYAFLRINEIYFIEYLPFAPISTIIGFTIL
                     ILFIVIASYMTGYKQLKNENIVESIKNETI"
     gene            complement(43004..43687)
                     /locus_tag="KR250_001048"
     CDS             complement(43004..43687)
                     /locus_tag="KR250_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0067012.1"
                     /translation="MEILKCENLTKIYGSNQTRVTALNNVNLSVQKGDFVSIVGASGS
                     GKSTLLHLLGGVDRPTSGKIYVEDTEISSLKEEALAVFRRRKVGLIYQFYNLIPTLDV
                     RKNILLPMLLDKRKVDEDRFSEIVSILGLSDRLNHLPSQLSGGQQQRVSIARSLIYRP
                     AILLADEPTGNLDRKNSEEIVDLLNLSNKRFNQTILLITHDEKIALEANRIVTMEDGV
                     IVSEKVVKK"
     gene            complement(44031..44984)
                     /gene="blaCDD"
                     /locus_tag="KR250_001049"
     CDS             complement(44031..44984)
                     /gene="blaCDD"
                     /locus_tag="KR250_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDD family class D beta-lactamase"
                     /protein_id="HBH0067013.1"
                     /translation="MKRKKNFIWIAILLVGVVMVMYYVGKKHNDINQKTDKNYIKSEL
                     NKSELNKSKIENNDKNKKEKINMVDYSDCFEGISGGAIFYNTKNKEYNIYNKELIETR
                     RSPCSTFKIVSTLIGLEKGVINSKESVMGYDGTEYPNKNWNKNLSLEEAFKESCVWYY
                     KKLIDKVDAKSVQNILDDLKYGNCDISEWEGDLKNGKGHLNGFWLESSLQISPKEQVQ
                     TMAKIFEGDTNFKKEHINILRDIMKIDINDKNINVYGKTGTGFDEKNKRVDAWFVGML
                     EREGDTYYFAIKSDDSNKEITGPKVKEIAINIIKKYYSVRE"
     gene            complement(45525..47639)
                     /locus_tag="KR250_001050"
     CDS             complement(45525..47639)
                     /locus_tag="KR250_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding domain-containing protein"
                     /protein_id="HBH0067014.1"
                     /translation="MSENKQVVNCDIIASNSKHNMCEYDAELLTFFPDEIAHLIEINN
                     KLDNAFKKAENLVDKLDKDYMDAKMYMVKNRGEIDPHEMFQNEQGLKQIDNYGAFMVK
                     VRDKINKIKDSPYFARIDFRLKDMDDESKYYIGRFAFDYEDELIILDWRSPIASMFYD
                     YEIGKAGYDAPIGWVDGEITRKRQFKIKNGKLEYALESSINIQDDILQKELSHTSDEK
                     MKSIISTIQKEQNQIIRNDKADTLIIQGVAGSGKTSIALHRIAFLLYRFKDKISANNV
                     IILSPNKVFGDYISNVLPELGEEPLCELSFENIAEVQLDRVINFESEKDPLEINDAKW
                     SERVRFKSTLDFVKLIDDYIKQMPNKIFIPKDYTFGSFTAKSDWIQSRFEAYNRYPVK
                     KRLEKVAEDIHYKFESDNIMEEDLPKVKSILKSLNGMLTIKNTLTLYKDFFKQMNISN
                     MFVMAEKKTLEWSDVYPFIYIHAAYEGIQEDKIIRHIVIDEMQDYTPIQYAVINLLFK
                     CKKTILGDFGQLVNPNHTHTLDDMKQLYNDGELVMLNKSYRSTFEIINFAKKIQDISS
                     LEPIERHGEEPALLKCNNEQDEINKIKIEIEEFKKSDNATLGIILKTDNDAKAVYDAL
                     REEYSVHLISSESSSFTKGVSITSIKMSKGLEFDEVIVPSVNNKTYYSDYDRSLLYIA
                     CTRAMHKLKLTYTCELTQLIDM"
     gene            complement(47723..48559)
                     /locus_tag="KR250_001051"
     CDS             complement(47723..48559)
                     /locus_tag="KR250_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0067015.1"
                     /translation="MSYKVINVITAIDYIEEHLSEKLDLDIVANAVHYSKYHLHRTFT
                     TSVGLTMHDYIKRRKLTEAAKLLVFSKKPIIEIALIAGYESQQAFTSIFKAMYKKSPN
                     KYRKEQEFYPLQLRFVLKKDNLFSKNVLELEKEIKLASMSDIPLWMNLVRLVIDGFPN
                     LQEEEYIYQLEQYILEERALILKLNNVAIANMVFNRETRSIDFFGIHPQYRNSDIAQV
                     FLKKVIEDFLIDTDISITTFREGDKADTGHRDMIKKLGFAEAELLVEFGYPTQKFILP
                     LS"
     gene            complement(49143..49505)
                     /locus_tag="KR250_001052"
     CDS             complement(49143..49505)
                     /locus_tag="KR250_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBH0067016.1"
                     /translation="MKIYDILTRFYIQDIEKAIPFYENLLKEKCSLRFSYKEVGLELA
                     QIGNVLLLSGSDDALKPFIETKSTFMVDSVDEWRSYLLDNGAVVVRDKKKVPTGYNMT
                     LRHPDGTIIEYVQHTKQD"
     gene            complement(49739..50203)
                     /locus_tag="KR250_001053"
     CDS             complement(49739..50203)
                     /locus_tag="KR250_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HBH0067017.1"
                     /translation="MTRISNITIAEQSEYCFLAIRKTIDFMVEFSEFSKQSFEKISKY
                     LEERGILSSGAPIVCFHNMDLVKLDVEVGFPVATYIDGKNEILQKIVPAQKIITAIDL
                     GLYELQDPTLEDLFSWANSNGYKLHGDIYYQYLNDINRPASEYLTKMMLPII"
     gene            complement(50632..52053)
                     /gene="nhaC"
                     /locus_tag="KR250_001054"
     CDS             complement(50632..52053)
                     /gene="nhaC"
                     /locus_tag="KR250_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HBH0067018.1"
                     /translation="MKERECRKASLFDAMIPITCLILFLSLGVLVYGSSPHVPLIGAA
                     AVAGLVAVYRLGFKWKELELSMFNSIKMAMQAILIIIIIGVLIGTWIVSGVVPCMIYW
                     GLKILSPNIFLVASTLVCAIVSLATGSSWSTMGTVGVALLGIGQSLGMPIGLIVGSII
                     SGAYFGDKLSPLSETTNLAPAMAGTDLFTHIKYMLYSTIPSLLICLVIYGVIGMKYSG
                     QALDIKQIELIRSTLDSTFNTLSPVLLLAPAIVIGLVVFKVPAIPGLIVGAVLGVLFA
                     VVFQGESMNTILDAAQNGYVSSTQIQEVDALLSKGGIMNMMSTVSLTICALSLGGILE
                     KTGMLEVVASSLLRLAKGVFGTVLCTMVTCTITNIIAGEQYLSIVIPGRMYNKEYKKR
                     GIHPKMLSRALEDSGTLTSPLVPWNTCGAYITATLGVSALTYGPYALLNIINPIVSLV
                     LIACKFKIARIEDEPETCLNFDV"
     gene            complement(52083..53147)
                     /locus_tag="KR250_001055"
     CDS             complement(52083..53147)
                     /locus_tag="KR250_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/ornithine racemase family PLP-dependent
                     enzyme"
                     /protein_id="HBH0067019.1"
                     /translation="MYPRLEIDIEKLKHNAKLISQMCHERNIKISFVTKSFCAQKEIV
                     EEICSEGIDHIADSRIQNLKNLQDINLPKILIRIPMLSELEEVINYCDISFNSELGTI
                     KKLNELCESKNIIHKIVLMFDLGDLREGYFYEEDFFNNVREIVRLKNIEIIGIATNLT
                     CYGAIIPSRENIGRLVSIAKRMEEKFGINLEIVSGGNSSSIHLLINNNMPEGITNLRI
                     GESILLGRETAYGENINGTYQDAFKLICQVVECKEKPSVPIGEIGVDAFRNKPVYEDK
                     GILKRAIIAIGKQDINIDSLIPIDTDIKILGASSDHMILDVSNTKYDYKLGDNLEFLL
                     TYGGIMSSSTSKYVSKKIIV"
     gene            complement(53132..54541)
                     /locus_tag="KR250_001056"
     CDS             complement(53132..54541)
                     /locus_tag="KR250_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate mutase L"
                     /protein_id="HBH0067020.1"
                     /translation="MENSKVSLKIDVLVAEIGSTTTVVNAFHDINTNNPIFLGQGQAP
                     TTVFEGGDVRNGLSGAIKDLANKLSVDDIEYNDMFATSSAAGGLKMTVHGLVYDMTVK
                     AAKEAALGAGAVIRQITSGRIKRTDLNKIKEINPNIILIAGGVDYGERDTAIYNAEMI
                     ASMNLGIPVIYAGNVENQEEIRLIFEDTNYKLYITENVYPKIDLLNIEPTRRIIQSVF
                     EEHITTAPGMKYIKEMVNENITPTPGAVMEASKLLYKNIGDLLTLDVGGATTDVHSVT
                     DGSDYINKILVNPEPTAKRSVEGDLGVYVNMKNIVEVIGKENLQSELSIDIDAVIENY
                     PPIPKSKEEILFVERLTKEAVVKAMLRHSGKIRNIYGTTGKVKIAEGKDLTEVRYIVG
                     TGGALTRLPSRIEILDNMLKYNKNNELLFPKEKTKILIDNDYIMASMGVLSKKYEEAS
                     LKLLLKSLNFEEERLCILG"
     gene            complement(54541..56745)
                     /locus_tag="KR250_001057"
     CDS             complement(54541..56745)
                     /locus_tag="KR250_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892628.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HBH0067021.1"
                     /translation="MLKENKKLDIDYILKDLDKYKPKRRGWVWREHLENLEMGPFKYK
                     DCTKPLKKSVGLPSSKYFNNIDPQPSPVITTEIASGRFEDDIRRMRMAAHHGADHLMV
                     IRTAGQSHFDGLIEGTPQGIGGVPITRKQVRAQRKALDFIEEEVGRPINYHSYVSGVA
                     GPEVAVMFAEEGVNGAHQDPQYNVLYRNINMVRSFVDACEAKKIMAFANIAQIDGAHN
                     ANATAREAWKVMPELMVQHALNSIFSEKIGIDKSNICLSTVPPTAPPAPCLKIDLPYA
                     VALRELFSDYKMRAQMNTKYMESSTREATVTHVLNLLTSVLTRADIQSTITPDEGRNV
                     PWHIYNIEACDTAKQALVGMDGLMDMIELKDVGELRDKARELKERAVLYMEEISEVGG
                     YFESVEQGFFVDSGNYPERNGDGISRKIKGGVGEGTVYEREEDYLAPVTAHFGYNNVA
                     QYDENAIDNPSMLIDGCTFENPDKIIYIDELDDFDNVENRLKESYEYRNGTKIKPEME
                     WCADGIIMITMMLPTDKRTAEFAALEFVKKMNLQEIEVISREVMHESEGTRIEVKGRV
                     PFDIDLNNLVIPEEPKVLTDEEIRADIEEKPMKIVSATVGEDEHSVGLREIIDIKHGG
                     IEKYGIECHYLGTSVPVEKLVDAAIELNADAILASTIISHDDIHYKNMKKLHDYCVEK
                     GIRDKVMIACGGTQVTPEIAVEQGIDAGFGRNSKGIHVATFLVEKRREMSNK"
     gene            complement(56739..57113)
                     /locus_tag="KR250_001058"
     CDS             complement(56739..57113)
                     /locus_tag="KR250_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine aminomutase subunit alpha"
                     /protein_id="HBH0067022.1"
                     /translation="MKKREDDFEVRRKHLQELSDDELKERFWSLATQIVEPMIELGKK
                     NTTPSIERSVLLRMGFSSLEVKPILEGVMERGLIGKGAGHVVYKLAKSKNITVREAGL
                     LLVKGEYWDEVTCLFREGVESC"
     gene            complement(57191..58621)
                     /locus_tag="KR250_001059"
     CDS             complement(57191..58621)
                     /locus_tag="KR250_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent lyase/thiolase"
                     /protein_id="HBH0067023.1"
                     /translation="MNNSTSMKDMSYQAVMGRNNEIMKNAIGLDYSSFEQEGIGFDYE
                     KMMSETGYTLQDIEAIQSQYAVGNTPLIELKNLTKLARKCAKEGKGARIFVKDEAMNA
                     SGSFKARRAATAVYHAKQMGYKGVIAATSGNYGAAVASQAAMQGLKCIIVQECYDSNG
                     VGQPEIIEKARKCEAYGAEVVQLTVGPELFYTFLVLLEETGYFNASLYSPFGIAGVET
                     LGYELAIQFREKYKKDPDIVVCTNAGGGNLTGTARGLIKAGSINTKVVGASVDLKGLH
                     MASDNQFNKKSFTTGHTGFGIPYCTWPDRSDVPRSAARPLRYMDRYVLVKQGEVFYTT
                     ELLAQLEGIERGPAGNTSLAAAFSLAQELDEDKAIVVQETEYTGAGKHICPQLTFARE
                     NGIDIKFGNPREEIAGVNLILPERPELLKCVDIDMNKIRKSFIKNCISNNHIDNIDKL
                     SNRDIEFLMEEVKSSRDFVVDVLNNL"
     gene            complement(58614..58922)
                     /locus_tag="KR250_001060"
     CDS             complement(58614..58922)
                     /locus_tag="KR250_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-ketopentanoate thiolase"
                     /protein_id="HBH0067024.1"
                     /translation="MDAKINDWVIIHNIVLTPEERAPQVPEDTKKVSLEMWVKGFIQS
                     DASIGDLVEVKTITGRLVKGNLLKINPYYTHDYGKCIPELLQIGIQAKEILFGGVYNE
                     "
     gene            complement(59043..60110)
                     /locus_tag="KR250_001061"
     CDS             complement(59043..60110)
                     /locus_tag="KR250_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-binding protein"
                     /protein_id="HBH0067025.1"
                     /translation="MRKVRVGIWGFGAMGIGMANMILKKEGIEIVSVCSRSTSGKSMY
                     DVLGIERGERPEVIINKNYEEVFREKSVDVVLLATDSFTKKAFDKINFLLNRKINVIS
                     TAEQMAYPQADDADLAKKMDEVAKENGVSILGTGINPGFVLDLLVLALSGTCEEVTSI
                     KAKRVNDLSPFGKSVMVEQGVGVTREEFIKGVEDKTIAGHVGFVESINMIADGLGWKL
                     DKIEQTKEPIMTTVDRKSKYGEALAGNVAGCRQCGYGYVNGEVLIEMEHPQQIIPEAE
                     GIKTGDYVSIKGIPNIDLQINPEIPGGVGTYAMIVNSIPLIINARPGLKTMLDIPVPR
                     AIMGDIRNQIEVELEEESKAN"
     gene            complement(60366..61739)
                     /locus_tag="KR250_001062"
     CDS             complement(60366..61739)
                     /locus_tag="KR250_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBH0067026.1"
                     /translation="MNEILENIDEGVHFVNKDGKTIIYNNSVQKMEKMNRHDILTNSF
                     FDIVNKMKINRSTLLEVLEKREVIKDNIQKYLDKNGKEITAINTTIPIDIKGEFVGAL
                     EISKNMTTIENLLNRNYKKNLVNKVSIRQKKGYEFDDILGESNLIKEAIAKSKKACKS
                     DASVFIYGETGTGKELFSQSIHYGSNRRNYPFIAQNCAALPESLFEGILFGTSKGGFT
                     GAIDRPGLFEQANKGTLLLDEINSMPIMLQAKLLRVLQEGYVRRVGGTKDIPIDVRII
                     ATTNESPKVILNEHKMRKDLYYRLNIIHIKIPPLRDRKEDIPLLCKKFISKYNKKLNK
                     KVVGISDEAIKILKNYDWQGNIRELENIIYCTMSMMEDEVQIETSMINLNDYYTLKEN
                     KESNNYSLNDLEDKTLSKIISEIEEKYIYESLEKASYNITKAASILGMNRQNLQYKIK
                     KYNIKIL"
BASE COUNT        20757 a        10543 c         7298 g        23452 t
ORIGIN      
        1 tgcttttgat gcttctaaat tttctgctgt tacttttcct actagttttg ttacttctgc
       61 tattttttct tctgcttttg tctttgcttc ttctttagcc tgtgatgatt ctaatgcagt
      121 tatctcactt tgtacttttg ctatatctcc atatcctgca aaactttgaa cttctgattc
      181 tgtccaccct gctgcctttg cttcttctat tgcatcagtt gccgctttta atgttgcttt
      241 tgatgcttct aaattttctg ctgttacttt tcctactagt tttgttactt ctgctatttt
      301 ttcttctgct tttgtctttg ctgcttcttt agcctgtgat tctaattcag ttatctcact
      361 ttgtactttt gctatatctt catatcctac aaaactttga atttctgatt ctgtccatcc
      421 tgctgccttt gcttcttcta ttgcatcagt tgccgctttt aatgttactt ttgatgcttc
      481 taaattttct gctgttactt ttcctactag ttttgttact tctgctattt tttcttctgc
      541 ttttgtcttt gcttcttctt tagcctgtga tgattctaat gcagctatct caccttgtac
      601 tgttgctata tcttcatatc ctgcaaaact ttgaacttct gattctgtcc atcctgctgc
      661 ctttgcttct tctattgcat cagttgctgc ttttaatgtt acttttgatg cttctaaatt
      721 atctgctgtt acttttccta ctagttttgt tacttctgct attttttctt ctgcttttgt
      781 ctttgctgct tctttagcct gtgattctaa ttcagttatc tcactttgta cttttgctat
      841 atctccatat cctttaaagc tttgaacttc tgattctgtc caccctgctt tccttgcttc
      901 ttctattgca tcagttgctg cttttaatgt tacttttgat gcttctaaat tatctgctgt
      961 tacttttcct actagttttg ttacctctgc tattttttct tctgcttttg tctttgtttt
     1021 tgacttatcg cttggttttg atgaagaact tcctcctcca tgtgaagagc ctccacttga
     1081 tgaacttcct cctgaagagt tagaacttgg actatttcca cttattgttg gagaattatc
     1141 tcccttaaca ctacttattg tacctttatt atctatagta gtccctttag aattatttgt
     1201 aactgtagtt atcttaccac cattagtcac ttttacttga cttgctccag atgctactgt
     1261 taaactacca atctcacctt tagaattatt ttctatcttg gctccatttt tatcattaac
     1321 ctttaagtta gttatttttc catagttaac aaaagttcca tcttttacat catctataac
     1381 tacatcatcc acttttgcat agttatttac atctccatta ggcatgttga tagttacagt
     1441 ctttgtatat gtaccattta agtttactgt tactgcttta tcagtttgta ttgtaaatgc
     1501 ttctttaacc tcactagttg gcttaaagtt tataacatca ttagcagtag ctttatctat
     1561 agctgatttt aattcagaaa ttgttttagc ttcaactgtt tttacttctt gtaatgactt
     1621 catttcatta aatgctgtct cattaccatt cccaccaact ttagttatct tatcaaaact
     1681 cttagagtta actagagatt tttgtgaagc tgataagttc tttccaacta aaactactgg
     1741 tgattgattt ttagcaccta atacacctac tgctagtgca tctataagtt ggtcttgttt
     1801 attcatacca tctttagtta cataagcatt ttttaaatct gtctttttat aaaattcttt
     1861 tattactttt gcatttgtat catttctatc tgttccacct agtcttgtac tatttggtag
     1921 tttattttta acagcttcag aaattgaact ctctccacct actacataag attgagttat
     1981 tccagcatct tttataaatt tgtctgcaac tgctgttcca tttttagaat cagcaagtat
     2041 tactggcata ttgttttgtg cagctggtgc tcctacactt acagcatctg caagtccttt
     2101 ttcaccatta actacagcta ttttcttaac atcacttata gcatctaatt tctttgctaa
     2161 ttctaaggat gtttgatatc tctcagctcc agagattctc tcaaaagata tttttaaatc
     2221 ttttatttct ttttctatat ttgtacttaa tacagaagtt ccacctatta agtatacttt
     2281 tttagctttt aatctttgta tttctgcttt cgtcttatca tttaatttat ctttacttgt
     2341 taaaagtatt ggagcatttt ttgcttttgc aaatggtgtt gcagatagag catctgctat
     2401 agatgagtcg tttacgatta ctacctcatc agcatttttc catccatctt gacttatttt
     2461 aacagcagtt tcatatcttt cttgccctgt taaatttgtt gccacagttt gtgcactagc
     2521 tatgcctgct gttgaacttc cagctatcat tgtaactgta agcattgcag caatcttctt
     2581 tggtattttc attttatttt cttccccctt gtccatttga tattacttaa ttttttaact
     2641 ttgttcccgt tctaattcta aaggatactt aatatctaat tttaaagagg tttctaacta
     2701 taatgatttc ataattaatt tgtaattatt ttgttacatt gcagtcatta tgtaaggaat
     2761 aaagaaattt tcttattgat tgaaaaattt gtaaatttat attttaaggt aagtttgatt
     2821 tttatgttaa tgaattgtta taaaaaatag ggttttgtat aaaaaaagta gcctttagaa
     2881 ttaaaagtgc tactcttttt acaaataaat tttattattt atacttgtaa ttatatatta
     2941 aataaccaat tttatccata tgaaaaacaa ataattgcga tattttttta attattcttt
     3001 atatttacat tttaattcaa attacttttg taaagattat atctgttgca tatactactt
     3061 taagtgctaa tatctctccc cctcaattag aatcctcgtt atctcaatag acgttttcta
     3121 ctgtcaaagt taccaacatt tgaaacttat taaattttta tataaaaaag acctagaata
     3181 taaattccaa gtcatttttt attaaattta ttttttacaa tataatattg tcatgattaa
     3241 agaacacaaa tcatacacaa attggtcata aatcttacat aaatcatcta caaaaaatac
     3301 attatttata ttaaatctat ataactaaat taagagcata ttgtacaata tgctcttaga
     3361 aaagatattt cttgtttata gtttatagta tttctctatg atttttattt ttatcatttc
     3421 aaaaataaaa attaagatta cagttattat catattttgt gtactatttt ctataatatt
     3481 ataatgaaac aatgaaaaaa caataattaa tgaaattacg tttatagccc cagaaatcaa
     3541 aaatgccttt ttcttattat tcatatattc cctctccaga ttatctaaat tagtctatat
     3601 caagcatttt taagtactta tcattaactt tatatatatt tttttcttta gacaaaatct
     3661 ctatctcttc aagattatta aacacttcca tcaactggtc aactaattca atttccttta
     3721 ttccagcctt taagtatcta cttttattct tttttatata attttctaat gtcatagaat
     3781 agttatcttt attgcaaata tattttaata tatgttcttc tgttgctgat agagttatgt
     3841 cattttcaaa caattcttct tttaatatac tttctaaaga ttttctatca tgcatattac
     3901 gcatctcatt aatagattgt attatagact tgagcataaa ttctataaat acagttaaat
     3961 tatttccttt gttttctaca tcaagtatag ccttataata cttagttctg tttttagata
     4021 tcatatatga tattgaaaaa tccttgaaag tatcatatcc tttatcaatc aaatatagat
     4081 atgataaggc tcttgatgtt cttccattac catcactaaa aggatgaaca tatacaaagt
     4141 aaaaatgaat tatagcactt ttaattaaat tacttacttt gctattttcc ataaaatcta
     4201 ttaatttact cataaagtca tatatcttca aaggttcaag ccctgtatgt ataatctcac
     4261 cttttgaatc acatacatat actttttcag ttctatactc atattcttct gaatccaagc
     4321 agttttcgcc caatatatga tgcaaatcat atataaattt gtgactatat aatttatcta
     4381 agttgtctaa tccatattct aaggctctat gattattgta aatcatatat tcacttttat
     4441 tacttggttt catctttcct ctaattatag attttgcaat ctttctagtt gaaaaagcac
     4501 cttctattac actagaataa taagactcta gtgccaaatc atttttatct tctattgttt
     4561 taaatatctt acttgatgtt aatttatcta tttcatttat taaatttaat atattttcag
     4621 tttcatgata gtatagttct ttattatcaa aactatttat agaaagtacg attttatttc
     4681 cagtaaagta tttgtttttg tattcttcta ttgtcagttt aatcaccacc ttagattatc
     4741 tttatctatt aatattaaca aaaagtatta atttttacac tatttttgat tatttgtttt
     4801 ataattatca aactcaattt agcgttaaag cataataaat tttaataaat aatgcaaaaa
     4861 tttaagaata agcatttaaa atatatgtca caggaagttt acaactatta tttataatac
     4921 attagttaca tattataata tctcgacaat gtcttaacaa tatctcgatt atatatttta
     4981 atatataact aatatgttat aatattttaa ttaaattaaa taaatttata tttttttgaa
     5041 atcacttaat attttatata aaaatgaaaa agcacctcat tctgattaaa aatctttctg
     5101 agatactttt taaaatatta aatactacat atagcttaca taagtttaaa atttaatcta
     5161 ttataaaatt accctaattg ccaaactttt taatataaat tttccaacat cctacaaata
     5221 taatcaatag atatagaatc ctaacttaca ccaatatata tttatttaaa atagtcctaa
     5281 aatacctgta atagctccca ctactatcag tataaacatt atttttattg gagaaagacc
     5341 tttttttaaa gcaaagagta ctaataaagt aagtaccaaa ggaattaacc caggaaatat
     5401 cttatcgagt aaatccgttt gaagactaaa tttaaaatca ccactagtat aacttattgg
     5461 tgtagataaa ttaataaacc tacctacaag tccccccatg actaaggtac ctagtataga
     5521 agccccttct ataaccttat ttactattcc accttgaagt atactttcaa cggctttttt
     5581 cccatactta tatccatttg tccacattcc aaaagaaatt gaagtcatta ttatatattg
     5641 agctattaca aataatatag gtcctgcaat tgatgctcct ccctcagtta aaccaataca
     5701 aacagccaac actataggag ttataactcc ttgagttaaa gtatcgccaa ttcccgctag
     5761 tggacccatc aagcctgttt tgatagcatt cataccatct ccacttattt catctgctcc
     5821 attagctttt tcttcttcca tagctattgt tattccatgt ataacacatc cacatatagg
     5881 ttctgtgtta aaaaatgcta gatgagtctc cacttcttgt tttaactctt ccttatctgg
     5941 atataatttt ttaagtactg gcaacattga atgtgaaaaa gctgttgatt gcaatctttc
     6001 ataattgtaa ttagattgtg caaagaaaaa ccatcttaac catgatttta ttacatcttt
     6061 cttgcttaac gttttcttag atacttcatt ttttatatct gacataatat tttccccctc
     6121 acatgtattt aagatgtagc actatctttt ccgtacttaa ttgaagtaat tgtaagagca
     6181 agtattactc caataacaga tattactatt atatctactt taaaataaac agccattagt
     6241 attcctaata tgaaaaaggg catagtaaac ttgtttccaa tcaattttaa attcatagca
     6301 atcccaagtg ctggcaacat agccccaatt acattaaata catgtaacac attatcattc
     6361 attacttcca acataccatc tatcgcattt ggaccaaaat aaacaactaa taatgctggt
     6421 ataaaactca ttgcaaataa cagtatctgt ggtggtacta tatttataaa tgctactcta
     6481 tcaacattcc ctttttgaac ctctctatca gcccaatgtg caaaaaaaga atttactgtc
     6541 atctttccaa tccaaagtac agttgcaaca gttcccaatg gtacagctat tgccattgca
     6601 gtacttacat ctagcttaga tgctatagct aaagcagtac ctacatatcc tgcaaaagct
     6661 gggtccccca tctgagctcc ccctgctgat ataaaaccta gatatatcat atttatacct
     6721 gctcctataa ttgctccttg aacagggtct cctaatataa ttcccgttac aaatccagcc
     6781 aatagaggtc tattccatgt aaaaaatcca acataagtca accacggact ccaagaaaga
     6841 taataaaaaa ttgctattaa tactgcttga attagtgaaa tgtgcatatt cttcctccct
     6901 attttctttt aaagatttac aagatagtct tctttacaat aatgttccta catcaatttt
     6961 ctttgcatct ggtactattt gtataaagca tttaacatct tttgatatta gctctctaaa
     7021 tatttctttt tcttcatctg acacagatat atttttataa aaactttttc taccaagccc
     7081 tgctcccata cctccaacat tcaattcctt taaatctacc ccgctattta ctaaacctaa
     7141 gacagtttta gggtatttga ccaatactat taaattttca tctccattat atgattttaa
     7201 gacttcagca cctgattcta catcatgtac ttcaacctta acagttgatg gagctgccat
     7261 ttgtaaaact tgaactataa atgggtcctt tactactaaa tcatccacga ttactatcct
     7321 ttttgctgat gtaatcttac accaagcagt aataacttgc ccatgtatta atctatcatc
     7381 aattcttgtt aatgctatat tactcataat tattcctcca gatttttaat ttattttaca
     7441 actttaaaac agttttaaca tctttgattc ctgatatccc aacttcctca cataaattct
     7501 tcaagtcttt taacttcata gtatctctgt ttaaaaataa ctctaaaagc ataggtaaat
     7561 ttacacctgt caaacattct aactcacata ccttagcaaa tttatttatt ataatagcag
     7621 atgtattaaa tggagttccg ccataaaaat caagcaatac taaaacccct tcatccttat
     7681 atttctcaat tccatcttct aactttctac taaactcatc tatgctgtca ccatggttta
     7741 atcctaaagt ttctacattt tcctgtttgc ctactattaa ttcagcactt tctaacattg
     7801 ctttagccat atttccatgt gtggctataa ttattcctgc catatgaatc atacctttct
     7861 taaccttttt tatatggatt attatttagt actctaatct aattatctat attaatcatt
     7921 atcttaccaa agaaaatatc ccttttgtat actttttcaa aataagctgg tacttcttct
     7981 aaatttattc tatgtgttac taatttttca acattcatgt cacccttttg catatgatac
     8041 aatccagcac tccattcttt tcctgggaat ggaaaggagt ttccaaacca agttcccttt
     8101 acagttaatt cacttcttac tatcttttca aattgctctc tagtcaaagc tacatcccca
     8161 tatggtacac ctgcatacaa tactgtacct ccctttttag ctagcaacag tacttgacca
     8221 catgtcagtg gagtaccagc cgattcaata acaatatctg caccatctcc atcagttaac
     8281 ctttttattt cttctacaat attcttttct tttgcattta tacaaatatc tgcccctaac
     8341 tcttttgcca agtctaactt ctcatcaaat acatccacag ctattatttt tgtagaacca
     8401 aatatttttg cccattgtat tgaaaataat cctattggac ctgtaccaag tactacaaca
     8461 gtatctccaa ctttagcttc tgacctaaaa agaccatgtc ctgctataca tactggttct
     8521 agcgcagcgg cagtctcata acttatctca tctggtattt ttattaaatt tctctctttc
     8581 acctttgtat actctgcaaa acaacctcca agttctttat ttcctattat tgctacatta
     8641 ttacatcttg aatacaagcc ttttttacat tcatcacatt caaaacatgg catagctgga
     8701 caaacagcaa ctctatctcc tattttaaaa ctccttactt ctttaccaac ctgtgccact
     8761 tctccagaaa attcatgccc taaaatttct ccaaccatat gtggacctgt ctttgaatat
     8821 tttgatatat cagaaccaca tattccagct acttttactt taattattac atcatctttt
     8881 tctaatattt ttggtacatc cacatcctcc actcttgtgt ctcttatacc atagaatcta
     8941 actgacttca ctattaacca cccctacttt ttatttgaga atatatccct attgtttata
     9001 tgttaaatat ctaaagcaac tttcatgcca atatatgtaa gcattgtatt catgcatttt
     9061 ataaaattat cattaaatgt gtgtcaaagt gtgtcaaaag tttcttatat taaaacttat
     9121 gtgtgtcaat ataaacgaca ctaacatttt atattagttc tcttattata agttttataa
     9181 aatgtacttt ttttgttatt tttataaaat tgttcggaaa tttcccttct atttgtgata
     9241 taatattaaa tataaggtat caatataaat tttcatgcga atatcagaac ttttttgaca
     9301 ctaatgcctt attcaatata ataatttaac tttttggagg ttgtttatga aaaaagtgta
     9361 tcaaggaaag actaaagatg tttttgaatt agataatgga aattacttac ttaaatttaa
     9421 agacgatgta actggtaaag atggagtatt tgacccaggc gaaaatagtg taggtctaag
     9481 tatagatgga attggtagag ctaatttaga gacatctgtt aagttctttg aaattctaaa
     9541 caatgctgga attaaaactc attatgtaag tgctaattta gaagaagcaa ctatggaagt
     9601 tctaccagca aaagtttttg gtaatggatt agaagttatt tgtcgttata gagctgttgg
     9661 tagtttctta cgtcgttatg gttcatgtgt tgaagaaggt gctaaactag attgttacgt
     9721 agaagcaact ttaaaagatg atgaccgttg tgaccctctt ataacatctg aaggtttaga
     9781 agctttaaac attatgactc aagctcaatt tgattcaatg aaaagcatga ctcaaaaaat
     9841 ttctaacata gttcgcgata ctatagctga aaaaggtctt gagctttatg atattaaatt
     9901 tgaatttgga ttctacaatg atgaagtaat cttaatagat gaaatagcat ctggaaatat
     9961 gcgtgtttat aaagatggtg taatagttga ccctatggat ttaactgctc ttttattaga
    10021 taaatagtat atacatataa tttacaaata taataaactt ataaataaga taagctatga
    10081 agtaaagact ttttaaatgt tgattatttt taaacatttt ctcgccttta atttcatagc
    10141 ttatttaaat taatatactc taattagtag ataatagctt taaccctaca attcccacca
    10201 caataaaacc tatacatgca attcttgata tactaatagg ctcatttaaa atagccatgc
    10261 ctacaatagc tgtaccgacg gtaccaattc cagtccatat tgcatatgaa gttccaagtg
    10321 gaagatgttt tagtgctaat gacaaaaaat aaaaacttac aatcattcca acaacagtaa
    10381 gtatacttgg agttaattta gaaaatccat gtgaattttt tagttgaaaa gcccaaaaca
    10441 cttcaaacaa acctgcaaca aacaaaaata accatttcat aaattcatcc ctcttttcaa
    10501 atatgtctct tatttttcca ataaaataac ctgagaaata ttataaaata tctcccaggt
    10561 ttttgtcctt ccgtgttcac aacaaaaact atgttgtggg ttttatctcg gaccagacca
    10621 atcattaata attgcggaac cctataaaac tctattaatt attctatttt atctataata
    10681 atactatcaa catatataaa aaaagtcaat atatgggttc catatataat aaaaataact
    10741 tttatatacc ttttaatatt cttattcact tctctccata cccatttggg tcaatctaag
    10801 aatatctaat ttttatttaa tatgttataa taacataaaa atattgaaag gagtaatttc
    10861 tatgaatttc tataaaattg cagtatgtca gatgattact acagaaaata aaattgaaaa
    10921 tatcaatcat gctgtagata tggtaacaga agctgccatc aatggatcta aaattgtagt
    10981 tttgccagaa atgtttaact gtccatatga aaataaatat tttcctaaat ttgctgaaga
    11041 atatccaggt gaaacaacaa caatattgag taaattagct gagaaacatg gaatttatct
    11101 agtttctggt agtattcctg agttagaaga tggaaaaatc tataatactt gctatgtatt
    11161 tgataaaaat ggagctctaa ttggaaaaca tcgtaaaatg cacttatttg acattgaagt
    11221 tacaggaaaa gttagtttta aagaatcaga tactcttaca gctggaaatg atgttactgt
    11281 aatagatacc gaatatggaa aagttggtat agctatttgt tatgatattc gtttccctga
    11341 gttatcacgt ttaatggctc ttaaaggtgc tgaaatcgtt atcttgcctg ctgcatttaa
    11401 tatgactact ggtcccgccc attgggagtt atctataaga atgcgtgctt tagataatca
    11461 gatattctat gttggtgctg cacctgctag aaatatgaat gcttcctaca tagcttttgg
    11521 aaattcaaga atttcagacc cttggggaag aataattgct caagctgatg aaaaagaatg
    11581 tattatttat gctgacattg atagagattt aattcctgac ataagacaac agttaccact
    11641 tcttaagcac agaagaactg atttatatga acttaatact ttgaaataaa acagaacata
    11701 accccataaa tcataaatta agtgttaaaa aaatttatgg ggttctcatt aatttgattt
    11761 aaaattaatt tcataaaaat aatactaata ttaatttaat taaataatac taataattac
    11821 taatgattat gctaattatc agcatttact aatttaatca ctattagtta tttcttttat
    11881 taaatatctt tacttttttt acttattatg tcttctagct ttacatttgg attatagtat
    11941 ttctttctat ttttagtcac atctttctat tgaattactt gcttaaaaca ccaatgcaca
    12001 cattccagac aatgttcaca attatgtaaa aatcttgctc taccttcttc aatgttgata
    12061 ttatctctcc tagttgatat tatctttcct aaaatttctt ttaattaaaa aacaacattc
    12121 gttgatatat cgagtatatt ttaagcaact gaaaaaaaca ataataattg atatatcaac
    12181 taattaatgt agattaattc atttttataa tttatatact ttttaattaa tctacctttt
    12241 taattaaaat tcttcctttc tatttatcca ttttaaactt aaattataag atttcatcag
    12301 ctgtgttttt gcattccaat aagtattaaa tatcaaatat atattctatt aaaatttcta
    12361 ataatttcat ccttgattta tttaatatat aaaaatatac taactttaat gattaagttt
    12421 tgaaatataa ctaagaattt gagattttat tgagattttt atactaaagt atacatacta
    12481 tacaagctta atttagaagg gagcattctt atgaacaata aaatactcgt cgtagatgat
    12541 gaggaaggta taataacact cttaaaagat tactttgaaa tgaatggtta tgaggtttac
    12601 actgctcaaa gtggaaaagg cgcattaaaa aaaatatcca aaaatccaga tattattcta
    12661 cttgatataa acatgcctga aatagatgga attgaagttt gtaatagaat tagagattat
    12721 gtatcatgcc ctattctatt tcttacagct agaattgaaa atactgacaa gataaatggt
    12781 tttcgtgctg gtgctgatga ttatattgta aaaccttttg atttagacga acttggagct
    12841 agagtatctg ctcatttaag acgtgaaaaa aggaagtcaa atcaaactaa aattaaattt
    12901 tataatgatt taacgataga ttacgaaaaa aggttaattt gtatagataa taatcagatt
    12961 tcactttcaa aaaaggaatt tgatatagta gaacttctct ctatgaatcc tggacaagta
    13021 tttgataaag aaaaaatcta tgatagagtc tggggataca atagcaatgg aaatagtgat
    13081 gttataatgg aacacattag aaagatacga atcaaactat caaaacatac tctggaaaat
    13141 tatatagaaa cagtctgggg agttggttat aaatggataa aataaataat atgtcattaa
    13201 aaaaagcttt ctttgttctt actttatctt cactaattat agcatcaaca cttactactt
    13261 tctcatacat tttgcttaat aatctatata attctataca agataagtat ttaaaaacac
    13321 ctataaatac gggttttata accatagttc aaaacaatgt gaaccaattt agtgattatg
    13381 ataaaacgtt acttaatata atcaacatac ttcagcttgt acttccactt atatttttta
    13441 taggactttt attattagct gatataattt tttacaaggt aaaattaaaa acacccatag
    13501 aaatactcaa taaaggtgca atggaaatat ccaacaacaa tttagacttt tgcttagagt
    13561 ataataataa tgatgaatta ggtaatctat gtaatgcttt tgaaaaaatg aggtctgaac
    13621 taaataaaaa caacataaag atgtggacta tgattaatga tagaaaacaa cttaatgctg
    13681 ctttttctca tgatttaaga aatcccctaa cagttctaaa aggttactca aactatttaa
    13741 caaaatacat accaacaggt aagcttagtg atgagaagat tttatcaacc acacaactta
    13801 tgtctgagca tattaacaga attgaatact atgtagaaaa tatgagtaat gctcaacgat
    13861 tagaagattt agttgtttca aaatctatgt cgaatataaa caagtttata gaaaacttag
    13921 atgaaaatat atctatcatt gctaaacaag aaggaaaatc ttttacactt aaaagtcaaa
    13981 taaataatat aaatctattt tttgatgaaa atatcattct tagagtcgtc gaaaatataa
    14041 tatcgaatgc ttttagatat gctagaaata atgtatccat acttatatat cttgaacaag
    14101 aactccttac ctttgtaatt gaagatgatg gtataggatt tagtaaggaa tctctaaaat
    14161 tagctttaag acctttctat agagataaga ctttaaatga cagcaatgca cactttggta
    14221 tgggtcttta tatctcaaaa atattatgtg aaaaacatgg tggctctatt tctattgaaa
    14281 ataattctac taacagtgct aaaattactg ctaaattctc aacaagaaat taaaatgatt
    14341 ttgtaagctt tcactgctat tatataaaat ttgttagttt agaatttata aaatgtattt
    14401 caatttataa ccaatatata tactattttg tagaaattgt aagagttgct tcaattataa
    14461 tattttggca aaaggcattt gaaacaataa tagaaatata tatttaaata tgtagaatag
    14521 tatgaaatta gaattttgga ggtgattata ttaaaagttt tgctaataat attacattct
    14581 ctcgtattgt gtttgcattg ctaatgctta gttttacatt ctgttcactg cctttctgga
    14641 tatgttattt ttggtgtgga attagtgata tatttgatgg tcttattgcc cgaaagttga
    14701 gacaacaaag tggaataggt gcaaagttag atagtattgc tgatttcatt ttttcggtta
    14761 caattttggt tactgtcgga aaaaatattg ctcttcccat gtatctatgg tggtgtattg
    14821 ttgcaatagc attgttacgg ttgattagtt acagtattgg tttttttaag tatcgcacat
    14881 tttcatcgct tcatactttg atgaataaaa tcactggaat ttctatcttt gcgtttccat
    14941 tactctatat ttgctttggt cttgatgttg caggaattat gatatgtatc gtggcttttc
    15001 tttcttcttt tgaagaaata gcaattatga taaaatcaaa agaattaaat cgtgatataa
    15061 aaagtatatt tatgtgaaaa cttagaattt gtgacaaaaa taatttatat aataatgagg
    15121 aagtaaaaca taaaaaatta tccacaagtt tatgtgtaaa atagttttaa agcaatagag
    15181 ttgtattgta aagcctttag tgcataaatt acattttaaa ttaaaaatga tactgaaact
    15241 gcttatcagc actgcgaatt atccgcaaat agtgaagact ttttagattt aagctaaaag
    15301 ttgggaaaaa attgagtttt aaaacttaca ctcttcttat atagaaaagg agagtgtttt
    15361 ttatgattat aaaagcaaaa caattatcaa aaatatatgg ttctaataac aataaagtta
    15421 tcgcacttaa taatgtaaat ttagaaataa actcaggtga atttgtttct gtaattggac
    15481 cttctggaag tggaaaaagc acacttttac atattttaag tggattagat aatcctacct
    15541 ctggacaagt gttacttgat gataaagata tatataaaca cactgaaaaa gaactatctg
    15601 cacttagaag aaaatccttt ggattcgtct ttcaacaatt taatctttta cctgttctga
    15661 cagcatctga aaatatttca atgcctgtac ttctagataa aaaacaacct gataaagaat
    15721 atttaaatga aatctcatca ttacttggaa ttgcagatag actcaatcat cttccccatg
    15781 aactttcagg tggtcaacaa caaagagttg caattgctag agcacttatt gcaaaaccag
    15841 atataatatt tgctgatgag cctacaggaa acttggacag taaaagtggc agtgaagtta
    15901 tgaatcttct tataaaaact tcaaaacagt ttggaaagac tttagtggta attactcatg
    15961 atgatagaat tgcaaaactt gctgatagaa aaatttcaat tattgatggt gttcttatgg
    16021 aggtgaaata gaaatgaaga attacctttc actttcaata aaagaattaa aaagccagaa
    16081 acttatgact acattcatca ttatagcaat agtactatca actattatga caactgtggt
    16141 tggacagtca attggtatcc tacaaaattt aagaattgaa caagcccgtt cttttaacgg
    16201 tgatagacat gtatcctttc accaacttac taaaaatcaa gttgatgaca taaagaaaga
    16261 tgatagagtt tatcaagctg gtacttcaat aactcttggc agtagtaaaa taaaggattc
    16321 tggtatctct gtacttgtaa aagagtatga taaaacagga ctttccaact accccaaatt
    16381 aatgaaatta aaatctggaa atttgcctaa agataaaaac gaaatagcct tagatgaaaa
    16441 cacactaaag cttatgggta taaaaccaag actaggagtg actataccta tgaatttaga
    16501 tatttcactt ttaaatgata caataccacc atataattac acagctaact ttaaattgag
    16561 tggtatttta gaggatgatt atacaggata tgtaagtggt atagtaaacg gtattgtagg
    16621 caaaggtgca tcagaaaatc tattacctaa gagatatatt ttatcctctt tggattttaa
    16681 aataaaacag caagagaagt ttcaagaaat agtaaatcaa ttagctaaaa aaataaatct
    16741 ttctcacaat tcaattcaat ataattggat ttacttaaat gctcttaaaa tacagtttga
    16801 aaaagatgaa aatagttcta atagtgatgg tatatctatg attatattgg tatctttatt
    16861 tgttgcctta ttggttttac tggcttctgg acttgtaatc tacaatattt taaaaatctc
    16921 tgttacaaag aaaattaagg agtatggatg tttaagagca ataggagctg aaccaaacca
    16981 gatatataag atagttattt tacaaatctt aattttatgt actattgcca tacctattgg
    17041 agctgtaatt ggaattatat cttctaaagg cataactgga atggtcacta atatcttaaa
    17101 tccagatata cttctagcaa atgataataa agaaattact gagcttatac ataaaaacac
    17161 aacagcatat atattcccat tagtattaag cactagtata tctttgattt tttcacttat
    17221 ttcagcatta ccatcagcaa tatatgcttc acatgtctct cccaaaattg ctatggctgg
    17281 cagtactacc aaaatcaaaa gaaaaattaa acgagaaaaa acaataaaaa actttgaaag
    17341 acatctagcc tggctaaact taaaaagaaa taaaggaaga actataataa ccatactatc
    17401 attatttatg agtataacag tattcgttgc actgagtgag ttctctaatg tattagatgt
    17461 atctaggtca gtaagtaatt taaaagaagg agatttttcc ttaacaaatg aaatatctgg
    17521 ttttgataag tcaatactag ataaaataga taaaatgaaa aacgtcaatc gcacttcctt
    17581 tataaagtac tctgaataca aacagggcga aatagataca gatataaatt ttgaaaatag
    17641 tggtgagatg ttaaaaatca ttggaattga tgagcaaact ttaaaagact taatgccttc
    17701 tattacagat tctatactag aagactttaa aaatggttct atctgtttta taaaaaatcc
    17761 gattgctatt tctacaccag gtgtaaaaac tagacataca aatttaaaac ctaaagataa
    17821 tatcacaatt aacaaaaaac aactagatat atatagtaca gtagataaaa tgttcttttt
    17881 acaaggtaat ggctggataa atggtgttga tgttattgta tatgattctg tttataacac
    17941 cttgacaaat aaaaacaagt tcaaccagat aaatatttat gctaaagata aatcaaaatt
    18001 agaacaaata agattgtcta ttgaacagat ttgtgaaaat aatcctggct ctcattggat
    18061 atcatatatt gaatcagata aacaacttaa agagagtttt aaacaaatag aatttctagc
    18121 atgggcagtt atattatttg tgggattgat tggtacttta aacatcatta acacaactca
    18181 tacaaatata aatactagga ctaatgagat tggagttaaa agagctattg gtatgagtaa
    18241 tagtagctta tataagatgt ttttatggga aggtgtttat tatggaattt ttgctactat
    18301 atttggttca attgctgggt atgtcagtgc tattattata aacatggcaa ctatagaaaa
    18361 gcttgatttt actaatatac caattacttc aattttacaa gctactatta tttctatact
    18421 tgcatgtatt atagctactc ttattccgtt aagaaaagtt aaaaagatga atataattga
    18481 ctgtatagat gtcaatttat aactatttta cagataataa ataaattaaa ttatcaatga
    18541 ctgtctctat tttcttttaa atctaactaa taaatctttt taactataat ataagcttta
    18601 aaaaaggcct gtctcaaaat agagataagc cttttttcat tgtcgaaata atgaaaatat
    18661 atttaacttc acaaaaaaag agtgcctcac gaattaaaaa tttcgctttg agacactccc
    18721 ctttaattta aaatatatag ttaaatgcta tgccaaaatt tcacacatgc tccacctgat
    18781 tttgaatttt ctattttaac agaacctcca agctgttcca ctaattgttt tacaatgtaa
    18841 agcccaagac cagaatgtcc tcctttagtc tgcctagctt catctcctcg ataaaactta
    18901 tcaagagcct tttttaaatc tttagagcta aaccctctac cagaatcaca aatctcataa
    18961 gaaatacaat ttttttcatc ctttacagta atactaatat taccacctga tggagtgtat
    19021 tgcagactgt tggatatgat attatcaaaa attcgtgtca acctatccac gtcgatttga
    19081 attggggatt gtatattccc ctgcattttc aatataagtt caacttcctt ttgatgagcc
    19141 tgtaattcat agtcttgtac cttttgcctc aaaaattcag gtaaatttat aggaacaaga
    19201 tttaattgta tatttgactt ttctaagtca gacgtatatt gcatcttttg gacaagagca
    19261 gcactttttt ctgtattttc tcttatcact gtaagatacc tatgaagttt ttcattgtca
    19321 tctgtattat taccaataag tgcatctgta tatcccaaaa taatagaaag aggagatttt
    19381 aaatcatgtg ctagagcttc caccatttca acacgttcct gctccatctt ccactgtgca
    19441 gataaagagc cttttaattc gtctttcatt tcagaaaatg cactacaaag tttaccgagt
    19501 tcattatctg aataatagtc gatttcaaaa tcaaggtcct tttcttttat cttcttggac
    19561 gcatcaatca atagatgtaa tggttgattg atattcttta caaagcgctt agaaagccat
    19621 ctagtaaaac ccacgatata tagaaatgga gaaaacagag ccattataat aactgcaaat
    19681 acaaatctcc ctctaatatt tgcaaaggtg attttcactt ggtagaatag aataacagct
    19741 ccctcaatct ttccattatc ccctttgatt ggaacagtat ggatataacc acctttacga
    19801 acagttttat tgataaaatt attaaataat tcttcttttg ttttaaatag ttttttgggg
    19861 ttagtaccat ataaaatatt gccattatta tccacaactt gatatagcat atcatcccca
    19921 cgaatcgttc tttttaaacc ttcttcattc gattgtgata acaaagttat gtttttttca
    19981 ttgatgtatt tttcaattct aggaacttgt cgctcataat agtttggtgg atagatatcc
    20041 ttatttaagg aatgaatata caaatagaga gcaaatacat aagtaatcac tgtggcaact
    20101 atactggcta ttataataaa aataaaagta tgtctaaatt gggtaataag ggattgattt
    20161 ttcatttaat aacctctctc tctttacatt ttattccact tatacccaat accccaaact
    20221 gtggaaatat attctttttc tggtgtcaca gcagaaaact tagctcgtat tttttttact
    20281 cgttcaacaa cagttgagga atcaccttca gaatcacacc cccatacttt ttcatagatt
    20341 tgttctcttg agaaaacttg tcctgcatgg agtgctagta attcaacaat atcatactct
    20401 atttttgtta aatcaatatt cttaccatca attttaattg cacgttcctt catatataag
    20461 cataacttcc caaaatacaa agtagatctc ttgttctctt cattgatata ctgtgaacgc
    20521 ttttccctac gcagattagc ttcaatcctt gccataagtt cacgcagtcc aaatggcttt
    20581 gttacataat catcccctcc aaaggtcaac cctttgattt tgtcggtttc tgattgcttt
    20641 gcacttaaaa atataattgg gcataaaaca tcatctctaa ttgcctgaca tacctcaaat
    20701 ccattcattt taggcatcat aatatccagt ataataaggt caggctcctg ttttgataac
    20761 tctatacctg cctgaccatc ataagctatc aacacttcat gtccttttgc cttcaattca
    20821 tcctcaagta gcattactag gtccatttca tcatcaataa tcaatatttt actcaatata
    20881 acaccacctt tctcttatgg gaaggtcagt attaaaacaa tattcgtcta ctaataactt
    20941 ttccttccct cccacatttt aaaccaaata acgcctccta tcaagaatat aatcaaacct
    21001 attgctacta ctataagctc tatactagct gtttgcatca ctttagagta aaatgcctca
    21061 gttgaaatag ttccagcttc tttaataaaa taaattcctg gaagcatacc tagttttact
    21121 ggccatgtcc atggaacaaa tgcccatact ttttcaccaa cgctagttaa tccaagtatt
    21181 gctgccatca aaattccacc gatactaata ccaatagatg cacccatacc ccatgcaaaa
    21241 ctcacccaaa gatgaatagc taatagtgga agtgtcccaa tcaccacaag tcctgctgct
    21301 gacaggaata accatattgt tgctccagat ggcactgcaa tactcattcc tatgcaaagt
    21361 atcaacgtag caataaatgt gcatatagtt aggcaaaaca gaagaaaaag aaattttccc
    21421 agatataatc taacacgaga aatacctaca cataaaaaac cattaaaatt tccagcaagc
    21481 tcttcctcct gcactataaa tcctgctaaa atacctactc caataggaat gatgaataca
    21541 gtccatactg taaaaaatcc ttcaaaagca aattcttgtg tacttccaga tttagttgtg
    21601 agataaaaaa ccacacataa tgaaaaaata ataggcataa aaagagtaag ccactggaca
    21661 gctgtcctct ttgctcgaag ccattctgat gataatagct ttatcatttg tcatccctcc
    21721 tattgaacca agctgcagta agtgctgtta atactataaa cacaaccaaa gaaacaacaa
    21781 tcccaatagg aatcactgaa gtattcaaaa gtgggtctcc agcttctaat atagtaccgt
    21841 taggatgtat gttgatgatt gggcatacca ttctaactgc ccaactccaa ggtactgcaa
    21901 tccaaaatgg ttttgtcgca gcaaggacac caaatatcat acctaaagct ccaactccca
    21961 tacttaggaa catacctttc catgtagcta gccatagctg aagtggaagt atagcaagag
    22021 aaacaaccca gcatacaata cttgccccta taatctgtcc aaaaggaact ggaccagctt
    22081 tcaatattaa accagttata attgttacga ctattaaaac tagcgttgaa ataaaactgt
    22141 agactgccat agctataact ttattaatcc aaagtgtcat aggagacaca ttgtgtgtac
    22201 gtaaagcacg ataatttcca gctttttttt cctggaaagc taccaaagta gcaaacaatg
    22261 caaatccaat aggcagaaat aaaaatgacc accagttaaa aattaatgct aaaagacttt
    22321 gccacgccca agaagttgtt tgagataatg gattttgcat gagtgttgat tgcattataa
    22381 atgcatatgc tgcaaaagac actgggaaaa aaacaataag tttacccata aatgttcttt
    22441 tatactttaa aagttctgat tgtaaagcat tcatctgttt tgtcctcctt taagaataac
    22501 atctgtaaaa aattcttcta gattttcatt agggtcaggc attccttgaa ataatagtcg
    22561 tcctccattt ataattccta tattatctac aacttgagca acctctgata gaatatggct
    22621 agaaagaatt acagttatac ctttttttgg aaaagaagca atcaattcac gcaattcttg
    22681 aatgccaaag gggtcaagac cattggttgg ttcatctagt attaacaact ctgggtcact
    22741 caaaagtgca atagctattc ctaacctttg tttcataccc atagaaaatt gtgaagctct
    22801 ctttttacct gtatttttca aatttactat ttccaacact tcataaattt tatctttagg
    22861 tattcccaat atttttgtat gtactaacaa attttcctct gctgtaaggt ttccatacaa
    22921 tgcaggggat tctattaaag aacctatttt tgcaagagat tctcgtttcc aaggctttct
    22981 atcaaaaata atacgaccat ttgttgggcg aagaagacca acaagcattt taagtgttgt
    23041 ggactttcct gctccatttg gtcctaaaag tccatatata ctatttcgtt caatttgtag
    23101 agaaacattg ttaactgcca tttgttctcc atatcttttt gataagtttt ttgtttcaag
    23161 aattaaatct ttcatattaa tttctccctt cttgtaatac cttaaatctg ttactaagag
    23221 aatactaaca tgaaaaaata acaattctat aacacctact aattttaata aaatttaaaa
    23281 taaaaaatag agttaccaat tttattttgg caactctatt taagtttatg ttttacagtt
    23341 atattagatt taagcttagt ttcattatca cactaacttc aaatttactc tataatcctt
    23401 aaatcaaaca ctaatttatt aatcaaacac taatctatta atttatattt ctttagacat
    23461 ctaatatagt ttgctactaa ataataatta tttatatcat atttatctaa tcctttaaaa
    23521 gaagttattt catcattaag ctcatcacta ccttctacaa aaacataacc tgtttcaaat
    23581 tccataccta caataatctt acagccacta taaatttcct cttgagtatc gtacaatttt
    23641 tttgcatttc cttcatttgg aattgtaaat acaataggtt ttaattcttt atcactggaa
    23701 atcttttgtg aaaaaggctt gtttaactta atattattga taactacatg tttaaattcc
    23761 tctgaagttt cttctactat tacatatttt cgctttaaaa actctattat ttctaaaaca
    23821 gacttagaat ttacttttaa aatactttta tatttttcat ataaagattt ccactgttca
    23881 attttttcct gtggtacttc ttggatatgt gtacttattt tttcttgaga tatttttttc
    23941 aattaaactc tcctttcaat acttaaatat ccttataaat ctcacatgac taaagttaca
    24001 aggttcctac ttaatagaat tttgcatact taaatatgtc ttattaattc tctacaggat
    24061 atctccgtag ttcctacgat tcttacataa gttacttatt ttaaattgtt attagttaga
    24121 ttataaaaat tatttatttt atcactaata taatactctt aaaaacttta caaatcaatt
    24181 taaatttgct accttttatt tataacaact tatagtacat ttctcctatg cttttcaata
    24241 tattactttt aaaaggaggt tttcattatg attttaaata gagctgagct tttccgagga
    24301 tattctttaa atagatttaa cgagatatgc aatatattat cagtaaataa aattaaatat
    24361 aagtataaag tgaaaaataa tgcttactct aaaaaacctt tttttaatga aataacactt
    24421 ggcaatttag gccaaaagga agatttctct tatgagtact taatttatgt tcataaagac
    24481 gattatgaac aggcagagtc tctaataaat tctaagttgc gatagattat ctataaaaca
    24541 tgacattctg ttgtcatgtt ttatatgtta ttatactttc aaatatgcta ctatattttc
    24601 aaaattgtgt gctttaaaac tagctttttt tagtaattta aagcttaaaa ctaaggagac
    24661 tcgttatatg aaaataaata taaaatcaaa gttaagtgaa tttattaaac agaacaatct
    24721 ttttgatgac agcagaataa tatcctatct tcctaatata acaatagaaa cagacaaaat
    24781 aaaaactata atgattaatg aggttgtccc aactaacacc aatgacgatt tttatagtgt
    24841 agataaagat gctgattatt taaaaactac tattccttta tttgacagtg caggaataaa
    24901 agtatctaac ataaatgata tcctagatat gggtatctat ataactaatg cagtaaaact
    24961 tcctaaatca gaatatacaa taactcgtga tacaattaaa ttacatatgc ctattttaga
    25021 agaagaaatt aaattattta aaaacctaga ggtagtaatg ttaatgggag atgttgcaaa
    25081 aaaatctttt aatatgatta ctaaaaagca tataaaaaag aatgtcatcc cttccatttc
    25141 aacatataaa cttcgtaata atgaacttta ttatgataat ttaagagtat ttccatcata
    25201 tattatgact ggtggaaaca tactgattga aaaatcaaaa tttgaaatgg catcagaaga
    25261 tataaaaaaa atgttcgaga ttataaacgg ttgatattac aaaataataa aaactaatat
    25321 aaaataaaaa gtgcctcaaa aacttctatt tatgaggcac ttttatttta tattagtgta
    25381 actatatact attaacataa tttattattt atattgttcc ttttaatctc tattccattt
    25441 agtctaggta acattactct ttacaagatt actttatatt tcttttatat ctctaacgtt
    25501 tttatttaat aaaacaatta ttaatacaag tcaaatatat ctatttatct aaaaatatat
    25561 cttttacagt aatatctgga tggtgatact tcttcctatt cgttaaaaaa gattttttta
    25621 cattaatagc ctcttttgga caccagtgaa aacaagctag gcatctttca cactcattac
    25681 cccactttgg ctttttatca actatactta tattctctac aggacaaacc cttttacatg
    25741 tcccacatct attacattta ttttctgcaa aaaatttagt agttgcaatt tgcatattct
    25801 tacctactaa acgaagtaca acactatcta tatgtgactt taaattgttt tttccttcta
    25861 tatttccaat ctccaagtta ttgatacatt ttgcaatctc agctacccta ttagtctgat
    25921 ttttcaatct ttcttcttca acatctggag gtgttatcaa agcatttcca ggcatattga
    25981 ttaaaaaacc agcagataat tttttccccc tttttttaag taacttatct atagtaaata
    26041 aactatgtcc agctacccca ttacaagtgg caatggcata tatatgagta ttaatattaa
    26101 aatttaattt ctctacaaaa gactttacta tatcaggaac atcagcaaaa taaacaggaa
    26161 aaacaattac caatatatct tcatttatgt ctatattatt ttcttttcta tgtatagcta
    26221 agggtataac ttctttactt ccatttaacg tttcttttaa aatatttgaa actactagcg
    26281 aatttcctgt tccagaaaaa taataaatct tactattcat tcttatccct ctttctatat
    26341 ttctacatca tcaatagtta aaaagtctct taaaatagga tttatattta ctttccaatt
    26401 ctatccaaaa tatcagtaaa caaaggcaat tcttcatcag agatagattt aaatgtctct
    26461 gttgcatata tacttgcaat ttcatttaat ggttcttcta attctctacc taaatctgtt
    26521 aggaatattt gaaacttacg tctatcttta acatctactt tacggacaat taatccctta
    26581 tttttcataa catctaagat tcttgtcgta ttagatttat ccttacatga tttttcacat
    26641 aaatctatct gagaaattcc atcttgctcc catagaaacc ttaataacgc ataatggtca
    26701 gttgttatat catatccatt ttgtttaaac atctttttta aatcatcttt tacttttaac
    26761 gaagtactta ctaacttagt tgtagcttca tatagtttct caaaatcttt cattttacct
    26821 ccatttaaat aatcccttgc tcctcccatc tatcaattat tcccttatat tgctcaatat
    26881 ctttaaaagc cattggcttt aaaacagcat ttatagcaat aaaattatag atttgagaaa
    26941 gcacaggtgg atttttcttt tcctctatgc aataaataaa ttttttagat accttttgta
    27001 tctttgattc tactttttca gttataggat tttgattaaa tccagttttt atagataaac
    27061 taccacaaac ataacctccc gaatctaaga ccatggattt tataaaagaa attgcttttg
    27121 aaccacgtgc attttcatat gtagtaactg ctatacatgg cttacgatac aataatcttt
    27181 caagcatcat atgtgctcta tctgtaaaca ccttgaaaag tccagttaca ctacttccat
    27241 aagttgggct accaaatata acaccatcac tcttagtaat tatatcatga atatactcta
    27301 ctttatcatt ttgattgata cacttgccca ttttataaca atttaaacaa ccaatacaat
    27361 atgatggatt gacctcactt aaatcaaaat aattaatttt tacatctggt attaatcttt
    27421 ctatatcttt atacaagtaa gtcaaaactt ttgatgtagc tccatttttt ctagggcttc
    27481 catttataac tgtaattatc atttctatat ctccttttta gttgttatat caacaataag
    27541 tatatcaact atatttttag ctgtcaatat aaaccattta tttaaagcta tacttacaca
    27601 gcttaaataa aagttttatt taactacatg tgcttattta atcaactata aatgaattat
    27661 ttttaataat tgacaaacta aattaatgat aatatacttt atgtattaca aatgtaagta
    27721 gaaggaggta tagtatgaaa aagttaccac atatttcaga agcagaatat caagttatga
    27781 aaattatttg gaaatatgct cctatcagca ctactgaagt aattgaaaag ttggtagaaa
    27841 caagtacatg gagtcctaaa acaattcaaa caatgttatt gagattagtt aaaaaaggag
    27901 ctttaacata tgaaaaaaat agcagagtat tcgtttatac tccacttgtt aaagaagaag
    27961 aatacgtagc tacagaaagt agttcttttt taaatcgttt ttataatggt actcttaatt
    28021 ctatggtact aaacttttta gaaaatgata aactatctga agatgatatt gaggaactga
    28081 gagaaatctt aaataaaagg acgactaagg gggataaata aatatgtatg ccccattctt
    28141 tatacgattt ttactcagta cattaacctt atcaatatta attgttgtaa ttttacttgc
    28201 caaaaaagta tttaaaaaac atatttctgc aaagtaccaa tacaatattg gatttatatt
    28261 gcttataatg ttaataattc catttgtccc tcttaaatat attaatcttg gaaatgtatt
    28321 taactattta tatactttta atcaacttgg aagcagtact aataggactt ctatgataaa
    28381 aaatagttca aattctgtaa ttaataactc aaatgcatta aacgattttt cagtatccct
    28441 aaatcaagtt aattttgata gtttaaatat tttattacta atatcatgga taattggaat
    28501 tttaatcatg atagctatta ctattcattg caacattaaa ataaaaaata tgaaaaaatc
    28561 aatacaaatt ttaaaagatg aggagatagt atttatattt gaaaagtgta aaaaagatat
    28621 tggtataaat aaggatttta tactatgcaa atcacctatt ataaatacac ctattacatt
    28681 tggatacttt aaaccacaca ttatattacc agataagtct atatctaaat tatctttaaa
    28741 agatataaaa tatatcttac ttcatgaatt acaacatttt aaaaataaag atatactgat
    28801 aaattatata atgtgctttc ttcaaatatt atattggttt aatcctcttg tatggtatgc
    28861 atttaaagaa atgcgtatag accgtgaaat tgcatgtgat atttcagttt taaaaaagct
    28921 taataaagat agtcatatag aatatggaca aactctaata aactttgtgg ataatatttc
    28981 aagaccatca aatcttacac ttacatcaga tattggtggt tcaaaaaaac aaataaaaaa
    29041 gcgactattg aagataatgg attttcataa tgaatcaaat acgttaaaat tcaaaagcat
    29101 tttaattttt acattgatag ctttaattgt atttggaaat attcccaaac tatctattat
    29161 ggcagatatc aacgaaaaat atagacctac aaatgaacaa actgtgtacg aggacttaag
    29221 tcagtatttt aaaggttttg atggaagttt tgttatgtat gatttaaaat ccaagcaata
    29281 tcatatttac aataaagaaa aaagtaaaac aagaatttct ccaaattcta cttataagat
    29341 ttatagtgcc ttattgggat tggaaaatgg gataataaca agaaataatt cacatatccc
    29401 ttgggatgga aagcatcatt ctaatagttc ttggaatgaa gaccaagact tattttctgc
    29461 tatggaaaac tccgtaaact ggtactttca gactctagat aaaaaaacag ggctaaaaaa
    29521 catacaaact tatcttaggc aaattggata tggcaattat gatgtttcag gtggtatctc
    29581 aaacttttgg ctagaatctt cattaaaaat atcacctatt gaacaggtag aattacttga
    29641 aagtttttat acaaataagt ttaaatttga agataaaaat atagaaacag taaaagattc
    29701 attgttaata tcaaaaaagg atggggtatc actttttggt aaaacaggca ctgccaatat
    29761 aaatggaaaa gatgtaaatg gttggtttat aggttatgtg gaaaaaaatg aaaatactta
    29821 tttctttgct acaaatattc aaagcaaaga ttattctaat ggtagttcag catctaagat
    29881 aacattatcc atattaaatg acaaaaaaat ctataatcct taatatattt actgtacatt
    29941 ctaaaaaatt cacataattt tcatattaat tatcaattaa attcatatac aaggcttatt
    30001 atataaacat agaagatagt aaaactagct taaaaacata atataatacc ctatacaaat
    30061 agtggcaact ctccctcaca tcctcaattg ttgccactat ttaatttttt gtatgaagtt
    30121 aaatatattt tcatcatttc aacaatttgg gtgcaataca atctctattt tgaaacaacc
    30181 tctttaattg ttttagagtt tatgaattat ttctcatact ttaaaaagat tgaattactc
    30241 ttgataatct tcttttgtag tcattacata tcacctacta ctcaacacat attctttgac
    30301 ctatttttac atccttgttt atatccaaaa tatttacctt cttaccatct ggaaacaata
    30361 ccattgtcaa agtagacttt cctaattcat gtattctctt taaatcaaca ttaacaagaa
    30421 cgtctccctg tttaaccata tctccttgtt taacagcaac atcaaagcca tctccattta
    30481 atgcaactgt atcaattcca acatgaatta atacttctcc attagttcct ttaatcccaa
    30541 atgcatgtcc acttgaaacc actgtttcta atttacctga aataggagaa acaatttttc
    30601 catctataat atcaatagca aaaccatcac ctaacatttt tttagaaaat gtttcatctg
    30661 gtacattttc taatgcaaca attttcccct tagctggaga cacaaagttt tcattatttt
    30721 cattaaacac taattcttcg tcttcatctt catttatagc ttctttaggc actccaaata
    30781 aacatgttac aataaagcag aatgcaacca caaatattga tactaataag tatgtgatta
    30841 aattatatcc atcatagata tacattaaat aagaaggtaa aactgctaat ccatatgaat
    30901 tagattgaat atgtaaaatt gataatattg ctgctcctaa acctgaagat gcacacataa
    30961 taatcaaagg ctttaaatta tatcgaagct gaatcccaaa taaaacaggc tcacttatac
    31021 caaaaagttg agatagcatt gctccaaccg cagtttgttt aacttgatta tttttagatt
    31081 ttttcataaa tgctagacat gttccaatat tcgcaaatcc atacattgca caaagagtaa
    31141 taagggcatt aaatcctgta tttgctaata gtgatgtttc aatcattaca taagtatgat
    31201 gtaagccagt aataacagct aatggatatg taaatccaat aataaatcct ccaattccta
    31261 aaggcaaatc aatcaataag ctgatgatat caaccatttt taattctatt gtatgcatta
    31321 tagggcctac acctaacaaa ataactaaca ttgttattag cataacaaaa aatggtgtga
    31381 aaataaggtc taatacatta ggcatatgtt tacggaacca tttttcaaga ttagctccaa
    31441 taaatgctgt aacaatagct gttaaaacac ttccttgaca acctacaata ggaataaatc
    31501 caaatgccat aattgcttca atttcatttg atgggtcagc aacagaatat gcattaggaa
    31561 gtattgggga aactaacata agtccaataa caagtccaat aacaggtgtt cctccaaata
    31621 ctttaaatgc tgaccataca ataattgctg gtaaaaaagc aaatgctgtt tctgtaagaa
    31681 ccgaaacaag tgtctgaata tacaatggaa tatttgcaga agacattcca aataatccaa
    31741 gtacattatc attaaacaaa caacccttta atcccaagaa taatcctgtt gctgcaataa
    31801 ctggaataat aggaacaaat atatctgcta gagttctcat aactttctta aatccagttt
    31861 cattattttt aactaattca tcttgttctt tttttgataa agtttttaat cccatttttt
    31921 caacttctgc ataaacttta ttaactattc ctgttcctaa aataatttga tactgtccag
    31981 aagtaaagaa aaccccttta acctcatcaa ctttttcaac tttcttatca ttaataattt
    32041 ctctatcttt aaccatcact cttaatcttg tggcacaatg tgtaatagaa ataatattat
    32101 cttcaccaat aattttaata agctcatttg ctattttatt atatttattc atttttctac
    32161 ctcactttca ataatactgc atgaaatggt ttcaaacgca ctgttttatt taatagattt
    32221 atatcatcta aattttggaa tacaatatca tatttttctt ctactcttat ttctacaggt
    32281 ttatcagaaa aattataata acaatcaata actaccaaat ctaattgcct tctatacgca
    32341 ataatatcat cactttctat atcaataggt tctattgaac cataaatcaa acaatcagaa
    32401 tactcactat cttgtctcaa ctttatcatc tttttataaa attctaaaat actctctgaa
    32461 ttgttaatct gactctgtgc attaatatct ttataatttt caataacctt taaccatggt
    32521 gttgtatctg aaaaacctgc attttcttta tctgcccatg gaaatggcgt tcttgcatta
    32581 tctcggcttc ttaaattcac aaattctaac gcttcttctt ctgagtaact ttcatctatt
    32641 gaacgatagt attggtcaat gctagataaa tcattaaaat catttatggt atctctttta
    32701 aaatttgtca ttcctaattc ctgtccctga taaataaatg gggttcctct taaaaagaaa
    32761 tacattgctc ccaacatttt tactgcatca tcattccttt cgtattgcaa aagatacttt
    32821 gttgtagctc ttggttggtc atgattttca ataaaattag ctgcccatcc atgttcctga
    32881 acatacattt gactatctaa aatgcatttt cttaaatctg gaattgtcca tgaaattctt
    32941 ttaaaccact cactaccaga agcaacatct aagtcagcat gtttaaagtc aaagaccatt
    33001 gaaaagaaac catcttctcc aataaaatca cctagctggt catagctaat acctgctgct
    33061 tcagcaacag tcatacaatc atatttttta aacgtatttt cttttaattc ataaagcatt
    33121 ttttctattc caggctgatt tctaactgtt tttgtacatt taacaagacc atcaacacca
    33181 tcactttcta aactttgaaa acttaaattc tttttgatga atgtaatagc atcaactcta
    33241 aatcctgcca ctcccatttg agaccaggta ttaatcattt ggtaaagttc acttcttaat
    33301 attggatttt cccaattaag gtcaggttgt tttttaccaa aagaatgaaa ataatattcg
    33361 tctctattct ctaccttttc ccaaacactt ccaccaaaaa cagacctcca gttatttggt
    33421 aaatcgtttc cttttttaaa aatataataa tcatgataag gactttctgg attttttaat
    33481 gcttcttgaa accaaggatg ttcatcactt gtatgattaa gaactaaatc taatataatt
    33541 ttgattcctc tatctttact ttctttaatt aactctttta aatcttccat acttccaaat
    33601 tcacttgcaa tatccaaata atcactaata tcatacccat tatcgtccat aggtgattta
    33661 aaaattggac ataaccacaa tgatgtaata cctaaatttt ttaaataatc taaacgagaa
    33721 ataattccct gtaaatctcc aattccatca ttattactat cttgaaagct tcttggataa
    33781 atttgataaa cgatttcttt ttgccaccat ttcttttcca taaattactc ctttttatat
    33841 gatatttctt ctttttaatt ctttataaat tccattatcg aatatgtcct cacatataga
    33901 tgtcgctata tctttaagtt gttgatgact atttttcatt gcaattgtaa cgtctgatgc
    33961 ttgaaacatg acaacatcgt tttgaccatc accaaaacat atggtttctt tttgagttac
    34021 tcctaatctt tcttgtaatc tcttaattgc tttgccctta tgaaaatctt tttgaataat
    34081 ttcataatac tgactagaaa tatctctctg tgctaattcc attttatctt gtagtatatt
    34141 tttcacttcg tcaaatacct tttcatttga ccataaacaa attttatgta tatcttgtga
    34201 cttatactct tcaatgttat tttcatatgt tattttttct tggatatgtt gtttatttat
    34261 acaagaatta gttcctttca actgattcat tgtttcaaat atttctttgg ctttttgatt
    34321 catgaaaact ttttcctgac tctcaatcga aaaagcaact tctctctttt ttaataaaca
    34381 aacaacttcc tttatcaatc tttgattaaa agattgatta taaagtaatt caccatgata
    34441 ttgaatgtaa ttacctcctc ctgcaatata tccatcaact cctaatgata aaacatcatc
    34501 ttgtattgta cccatacttc ttccagtaca aataacaaca ctgcaatgat tcttttgaca
    34561 taaacgtata gcatgctttg cagattctgg gattccatat acctcatctc taagcgtacc
    34621 atctatatca agtattaata atttgtacaa tttcatcttc ctttcttact atcaaaatca
    34681 taatagcatt caaaaaaaaa gatatcaata ttttgatatc ttttaaaaaa tatgttcatt
    34741 tttagcacaa attttcattt tcggggaata tgttcacttt ctaaatacat cttgtattta
    34801 atataaagca gttcaattaa aataaaataa ggtgttgtta catcgtagtc cagttgttta
    34861 tctacagaaa tagatataga ttgaatatac aggttttcat cacataatct tgctaaagga
    34921 ttacttatca tttttgtaat agaaaccata taaattcctt tcattctcat ttctttagca
    34981 aaatcaataa ttgtagatgc atctccattc aatgaaataa agaaaatcat atcatctgtt
    35041 tttataaaat gattaagtgc ctgaatcata tcataaccat aaacatcata tataattttc
    35101 cctgttggta agaaaatcct tttcatctct ttagcaactc tttcctgaga ataaccttct
    35161 ccaaagacga ttatacgatt tgcctttttc attctttcaa acataatcga acaatccttc
    35221 ttctctatat catctatcac cttattataa caatccatta attggtgttt actttgattt
    35281 ttatattcta cttctgtttc atttaagcgt ataataactc ttaattcact atatccaggt
    35341 aattgtagtt tttgagcaaa acgagaaagt gcacttgtag atatatggta tttgtctgca
    35401 aattcatcta tagaaagttt aatacaatct tctttatgat taagaataca ctcacagata
    35461 tacttatcat ttgctgtaaa tgaattataa tatttattga aaagtccttc aattttcatt
    35521 cttttctcct tctatactct atgtaattca tatgaagcaa atctaataga gcataaaatg
    35581 ctcctacgac ttcataagat atagcttgta atgtatcaag tttttgcgtt tcaacataca
    35641 gattatactc acaaagtgtt gcaattgtat tattatcaag cctagttaaa gaaatcgtat
    35701 gtatatgatt gttgataaat tttgcaattt caattcctgc ttttgtttct ccagacaaag
    35761 aaatgataat aaataaatca tttttttgaa atgattctct cataaattca atttcaccta
    35821 tatcaaataa gtcaataaca catttaccta ctgaaagaaa tattctcttt atttcttgag
    35881 ctaaactttt ctgttcatta cctgtcccat aaatataaat tgtttctgca tcatatatta
    35941 ttttacaaat atcttgaaat gatatttttt gtaattcatt cattaatagt cgataattct
    36001 cgcaaacatc atgaaaatta ttatcattat taacaaattc tagttgatta tttttaagta
    36061 aatactttaa atcagagaat gaatctaatc caatttttct acaaagcctt aataaagtag
    36121 ctctagaaac attacattca tttgcaattt tttcacacga aaaattacgg tattctcttt
    36181 gacaaatcaa tatttgttta ataataattt tatcattttc atttaaatta tcataatttt
    36241 ggttaactaa cgattctaat ctcatttttt ccactcccta aatattattt taatatacat
    36301 tagttgcttt ttctagggaa gagttttaaa taataaggat aaaataatca tttgatttta
    36361 tttttattaa aataaaaata acctgctaaa agcaggcacc aaaaagtact tatcctttcg
    36421 aacatgtact ttagatattg ctttcttcgg aagcttcatc tcttcatact ctaaaatata
    36481 gttttttcta actcaaaagt attttccatg gtagaaatta ctttaattca tctgccattt
    36541 actttttatc ttatttaata attactatta cttgaatatt tacttcttta agtaaatttc
    36601 atagttaaca aagctaaaac tattattata gatgttaaaa tacaaaattg taagtattgt
    36661 tataaatatg cttacagcta caaatataca tgatatatta aaaagaagct gtaaattatt
    36721 atcatacata caagcaacta tgcctctatt aatccatgtt aatttttatt atttaataca
    36781 aggagttcca tgagacatac atatccattc atattttaaa ttatccaatt ctcttataga
    36841 tttcaccatc tttttataat cccaattcca tggattagga taaggaacta tatttccatt
    36901 cttatttttg actaaatcac ctgcaaataa tacagcttca aaaatcatgc agacatgccc
    36961 tggagtatga ccaggagtat ggataatttt tatattccca atctgcatat tctctccata
    37021 aggttcaaca tccttaatga gccttctgga aattgccttt ccaataaact tcttaaaccc
    37081 aggtctatcc ttctctccta taataaaagg tatatcctct acatgagccc atacttttgc
    37141 tcctgttttt tctttcaata atttaatatt agatatatgg tcaacatcat gatgagttaa
    37201 taaaatatgc ttaatatctg taagtttaat gccaagtgtt tttaattctt taattatatt
    37261 tttccccata aaaggaagac ctgtgtctat aagaataatt tctttatcaa acacaataaa
    37321 acaatttgag ccacgagtac tatctaacat taatacattt tcttttatct tcataaataa
    37381 attcccctta tttggtattt tacctatata attaattaat aaagttataa atagttataa
    37441 tacttgatat taattttatt ttttattaaa gtatactata ggtatacaag tattatattt
    37501 acataagtat accatcagta tactttttta tcaatagaaa ggaataattt atatgtcaga
    37561 attatcaaaa agagataaag aaaaaataca aagagaaaat gagattattg ataaagcaga
    37621 aaaattattt tgtcttaatg gatttgataa tacaactatg aatgaattag caaaagaagt
    37681 tgaatatact aaacgcacca tatacaagta cttttcatgt aaagaagatt tattctttgc
    37741 tgttgtctta agaggatata aaagactttg ggataatgtt aaaatagaaa gtgcaaaagg
    37801 caaaacaggg tttgaaaaaa taaaactttc atactttgct tttcataaat tttactctgt
    37861 agaaccttct ctattatgcc ttatgggaat gattggtatt gtaaactctg aaaaatcgga
    37921 tactgatatg ctgtttaaag aaaagttttt ttcttttaat aaatttatgt ttgatgaaat
    37981 acaaggaatg tttgaaattg gaaaaaatga tagaagtatt aggcatgata ttgaaatacc
    38041 tactcttatg tattcatcaa tatttacact tacaggattt tttaatttac tatctgtaac
    38101 aggaaaatca tacctgaaca actttaatat agatgaggaa aaatttattg aaacaactct
    38161 tgctctttta atagattcaa ttaaagcata aacctaagtt tataaattaa agtttatata
    38221 cttaaatgat aattttttct atctctaata taaaaaatat taaacataaa actaataact
    38281 tagttaatgg aataactaaa ttactgaaag gattaaaagt aaatggctaa aatacttctt
    38341 ttagaagatg atgatacaat tacatttgga ataaaaactt cccttgaaaa gaaaaatcac
    38401 actgtatgtt gttataatac gattgctgaa gctaaaaaaa attttgataa aactattgat
    38461 ttaattctgc ttgatttaaa tctacctgat ggtattggat acgagttttg ccaatatgtt
    38521 aaaagtatag aagatactcc agtgatattt ttgacagtcc gtgatgatga aaaagacatt
    38581 atacgtggat tagatatggg tgcagatgac tatgtggtta aaccgttttt gctctcagta
    38641 cttcagtcca gaattaatgc agttcttaga agaactcatt caaatactaa caatactatt
    38701 acatgtgaaa atataagctt gaataaggtt gaagtcaaag catatttaga taatgttgaa
    38761 gtacctttga cagcaggaga atataaactt cttctaagtc ttatggaaaa taaaaataga
    38821 gctatgactc gtacaaagtt actagaaatg ctctgggatg tagatggtaa tttcgtaaat
    38881 gataacactc ttactgttac aataaaacgt cttagagaaa aattaaaaaa tccaccttgt
    38941 ataaaaacgt taagaggcat aggatataga atggaggaca gctagtgaaa actccaagaa
    39001 atatcaacag tacagttgtt tttataatat tctgcttaat tgtaactttt ttattgagtg
    39061 taattctaac aactataact tcttttaatg aatacaatgt actgtctaag gctcttggtg
    39121 catcatatga gtacaatcct aagtctacag aaagtatact aaaatcactg aaaaattcta
    39181 gtgataagga ttttaaaaca ggaaatgaaa ttttatcaaa acatggatat tctaaaagta
    39241 cattttggag taaaaacttt ttttattttc tagtgatatc gtttggtata atttcaacaa
    39301 tggctacagt tatatatata atcaaacata gaacaaataa gcaaaaactg agtagaataa
    39361 acaatcttac tcaatactta gaagaagtga atttaggaaa agagtctgta cttttacgat
    39421 gtgaagatga attttctcac cttgaagatg agatatataa aacagttggg gaacttcgtt
    39481 gttcaaaaga atttgcttta aaagaaagac aaactctttc agataatctt gcagatattg
    39541 ctcatcaact taagactcca attacttcta tgtcacttat ggcacaatta ctttcagaaa
    39601 attgtccaga tgaagaaata gattatatta acagacttac aaatcaaatc tcaagacttg
    39661 agagactagt ttcatcttta cttacattat caaaattgga tgcaagtact ttaatatttg
    39721 aaagcaaact tatagatgta cattctcttc ttacttatgc aattgagcct atagaaggtt
    39781 ctttaagaga aaaacatcag acatttacta tcgaggcttc tccttcggta gaatttgaaa
    39841 tcgatattaa ttggacttta gaagctcttt taaatattct taaaaattgt agtgagcata
    39901 tagaagatgg tggatatata gttgcttatt atagtcaaaa tcctctatat gttgagatta
    39961 tcatagaaga taatggtaag gggtttgctc aggaagaact tccatatatt tttaatagat
    40021 tttatcgtgg taaaaatgct agtaaggaca gtattggtat agggcttgct ctatcaaagt
    40081 ctattataga acgtcaaaat ggtactctac atgcagaaaa ttgccataat ggtggtgcaa
    40141 gattcataat taaattttac acataaaatg ctggattata ggtaaaatac ctataatcca
    40201 gctatatcta tattctatgt tatctattgt tttagtaata tacttctatt tacatttaag
    40261 gttttattaa acaaacaact taactatccc tatacttgta agtagtctta aatcgaaacc
    40321 tttaaataag ttcaaataga ttttgtttat attgtttcat tttttataga ttctactata
    40381 ttttcattct tcaactgctt gtatcctgtc atatatgatg ctattacaat aaaaagtatc
    40441 aatatagtaa aacctattat tgtagatata ggtgcaaatg gtaaatactc tataaaatat
    40501 atttcattta tacgtagaaa agcatacact attcctattt gcacaggaat actaagtagt
    40561 aatggtgtaa ttcctaagaa taaaccttcc aaaataagca tcttttttat gccttctgga
    40621 gataatccaa cagaacgcaa catagcaaac tcttgtcgtc tacttctaat atttcctgac
    40681 actgtagcaa gtacatttga taatccaatc atagccaata aacctgtaat aaagcctact
    40741 attaaattca tggtctttat tccactagcc ttttgttttt cactttctaa aacatcctca
    40801 atactataat ctcctgaacc atagtcttta ttataaatgg actctaattt ttctcttact
    40861 ctaggaatgt cctcacgatt atttacttta aatactccta tcagcctatt tgaacgtaac
    40921 ctacttactt catcataatt tttagatatt tcctgtacca tagacattgg aactatttgc
    40981 attaatgtat actttgaact agtatctact attttaggaa gtgtgcttgt aatagctccc
    41041 actttcatgt tgtaagtata atccccttta tcttcgtcat atactttttc atttaattta
    41101 atgttatctc ctattgaaag atttaaataa tctatatata ctttatttct acgtgtacta
    41161 cgattaatat cttcctcctt attatataca attgaggttg gattttttgt attataaaat
    41221 aactttgggt ctacagaaat ttgcctacaa tattctgtaa aactcttatc atcaagccca
    41281 attatatttg aaattatacg aaacttgcca tcacgttcta taggagagta ttttttagat
    41341 gaaactatac ttttaaatcc tccatttttc gcaagttctt ttgatgcatc tttttcagtc
    41401 agccaagtgg aagcagacat tttttgataa tatagtgaac tttttacacc ttctgtatta
    41461 tctaattttt caatgaaagc agactctata ggttcactat tttccaaata aactgctata
    41521 tctctttggt ccttataatt atcacttcca taaacagctt ttgctccttc ttgatttgca
    41581 attatatgca aaaaacatgt aagtaataaa aaagataatg ttaaagatat tgttgcagta
    41641 cgataagatt ttttacgagc acgtagtgcg tttgatgcaa gttctccttc tattccaaat
    41701 atctttccta atgaatattt atgcgacttt ttgattttaa cactatctcc ttgtcgtatt
    41761 gcttctatag gagatatctt tgatactttt cgtgctggta taagtgcaga aatccagact
    41821 gttactattg taagtaaaac tgctggcaga aaagcaggaa taccataagt aaataccact
    41881 tctggaacat cagtatgata attaactgaa ttaacataaa ctataagcct attacacaat
    41941 aaccatccta aaaataaacc tattggtaat ggtataattg tcagtaaaaa gccttcaaac
    42001 actacagacc ttttaatttg ttttggtgat gcaccaacac tagataatat ccctaactga
    42061 gtaagtctag catttgcaga cagtgaaaac gcgttgtaaa tcaccattac aaacacggct
    42121 actgtaaaaa ttgctattgt aagatacata ataggagttg aaaattttga tatactactc
    42181 atagatgctt tctgttctgg tgaaaagact aacatcttta aaagataatt actattgtat
    42241 ttaagattgt attttccata ttcattagtc ttccatccca atgatttagc aagttttgga
    42301 agttccttat acgtgtctct tatattttta aatcgcaagt acacagttat actatcatta
    42361 ggttttatac ttgttttatc aagatatcca agagctgtat atgcaggtat tactgatgaa
    42421 gtagaaacat ctacctttcc tactattgtc aatgtagcct ctgaaatttt tttaaaactt
    42481 tcccctttcg attttggggc tatgggttca agaggctttc catttaagat tctatatcct
    42541 attgggagag taattttatc accaattttt acacttggat tattctcaaa atattgtttt
    42601 gataatgcaa tttcattagc attatttggt cgacgaccct ctattatagt atccttttct
    42661 ggcatagaat tccaatactc tgatgtagca tttctataaa ttatataatc tcttttctca
    42721 tcattaattt tgcctacata ccattgtcct tttaccatta catcatcaac tgaagaaaaa
    42781 ttttcaatta atggtaagtc acttccataa gtatagtcaa acaattcacc atgccagtta
    42841 ccttctttct caattgaaag tcttataaaa tctgtccaca tggtatacaa cagtccacac
    42901 aaagaagaca tcatggttgt agtaagtaat atagctaaca ttatagctat actactacgt
    42961 ttattgcgtc gtacaaagtc taaggtgtat tcttgaagaa tattcatttc ttcaccacct
    43021 tttcagagac aatcactcca tcttccatag ttacaatacg atttgcctct agtgctattt
    43081 tctcatcatg agtaataaga agtattgtct gattaaatcg tttatttgat aagtttaaca
    43141 agtctacaat ttcttctgaa ttttttctat caagattacc agttggctca tctgctaata
    43201 aaatagctgg tctatagatt aaacttctag ctatagatac cctttgttgt tgtccacctg
    43261 aaagttgact tggaagatgg tttaatctat cagataatcc caatatagat acaatctcag
    43321 agaatctatc ctcatccacc tttcttttat caagtaacat gggtaataat atattttttc
    43381 taacatcaag ggtaggtata agattataaa attgataaat aagacctact tttctacgtc
    43441 taaaaactgc aagagcttct tcctttaaac ttgatatttc tgtatcttca acataaatct
    43501 ttccagatgt aggtctatct actcccccaa gcagatgtaa caatgtagat ttgccagaac
    43561 cagatgctcc tacaatggat acaaaatcac ctttttgaac tgataaattt acattattaa
    43621 gggctgtcac tctggtttga tttgaaccat atatttttgt gagattttca cattttaata
    43681 tttccatata caatcatctc ctttatggtt aaattttagc aacctaaaat gacattatag
    43741 tgacatgaat acaaatataa attactatta aaaatattgt ttagtttttg cacattattt
    43801 aaaattataa tataaaacaa aaactatgca atatatttta tttatacttt taatatatta
    43861 catagtcttt aaaaggtatt aactttaaat ctataggtat tcaatttaat aatcaatagc
    43921 aagtatttta tgtactaaag gagttttgct ctatatagac tcaatttagt aaaattattt
    43981 aataaatatt acagactata ggtaaataat atttacaaat aaaattaagt ttattccctt
    44041 acactataat atttcttaat tatattaatt gcaatttcct ttacctttgg ccctgtaatt
    44101 tctttattgg aatcatctga tttaatagca aaataatagg tgtcaccttc acgctctagc
    44161 ataccaacaa accatgcatc cacacgttta tttttttcat caaatcctgt tccagttttt
    44221 ccatatacat ttatgttttt atcatttata tctattttca taatatctct taaaatatta
    44281 atatgctctt ttttaaagtt tgtatctccc tcaaatattt ttgccattgt ttgcacttgt
    44341 tcttttggag atatttgtaa tgaagattct aaccaaaatc catttaaatg tccctttcca
    44401 ttttttaaat cgccttccca ttcgctaata tcgcaattcc catactttaa atcatctaaa
    44461 atgttttgga cagatttagc atcaacttta tctatcaatt ttttgtaata ccaaacacaa
    44521 gactccttaa atgcctcttc taaactaaga tttttattcc aatttttatt tggatactct
    44581 gtaccatcat atcccataac tgattctttt gagtttatta cccctttttc taaaccaatt
    44641 aaagttgaaa ctattttaaa agtagaacat ggtgaccttc ttgtttcaat taattctttg
    44701 ttatatatat tatattcttt atttttagta ttataaaaaa tagctcctcc actaatacct
    44761 tcaaaacaat cactatagtc aaccatatta attttttctt ttttgttttt atcattgttt
    44821 tcaattttgg atttatttaa ttcagactta tttaattcag attttatata attcttatca
    44881 gttttttgat ttatatcgtt atgttttttc cctacataat acattaccat aactacacca
    44941 accaataaga tagctatcca aataaaattc ttttttcttt tcataattta tatcatctcc
    45001 catatttaaa tttataacct actattaaaa tagtagacat tatttgtaat atactccaat
    45061 ctaggttcta cttataagaa acaagactaa atgaatagct gtatctctta tattttcttc
    45121 acctagtaca attatgttga ttaaacctat tgcgaagata attaggtaac tattaataac
    45181 atcctgtcct ccttttaatt tgtgatataa taattgattc cacatttaaa acttactatt
    45241 gtaatattta aatattacaa tagtaagttt taaatgtcaa gttagtttag gaaagttatt
    45301 ttaattgtat aattgttatt acatttataa aatatggcat gtttaaaatt attttgctat
    45361 tgggaggatt aagataagca gtattataaa agctataaat tataaaattt aatatataat
    45421 ttatagcttt tacttttaca aatattgttt ttattgattt attaatgaat tttattgaca
    45481 ggttatattt atataagtaa acacttacat aataagtata taatctacat atctataagt
    45541 tgagtcaatt cacaagtata tgtcaactta agtttatgca tagctcgtgt acaggcaata
    45601 tataacaaac tgcggtcata atcactataa tatgttttat tatttactga aggtacaata
    45661 acttcatcaa actctaaacc ttttgacatt ttaatagaag ttatggatac tccttttgta
    45721 aagctagaac tttcagaaga tattaagtga acactatatt cttctcttaa cgcatcatat
    45781 acagctttag cgtcattatc tgtctttaat ataataccaa gagtcgcatt atcactcttt
    45841 ttaaattctt caatttctat ttttatctta tttatttcat cttgctcgtt attacatttt
    45901 aataaagctg gttcttcacc atgtcgttca ataggctcta atgaagatat gtcttgaatc
    45961 tttttggcga aattaataat ctcaaaagta gaacggtaac tcttgttcaa cataactaat
    46021 tccccatcat tatatagctg tttcatatca tctagtgtat gagtatgatt aggattaacc
    46081 aattgtccaa aatctccaag aatagtcttt ttacacttaa aaagtaagtt tattacagca
    46141 tattgaatag gtgtatagtc ctgcatttca tcaataacaa tatgccttat aatcttatct
    46201 tcttgtatac cttcatatgc agcatgaata tatataaatg ggtatacatc tgaccattca
    46261 agtgttttct tttctgccat cacaaacatg tttgatatat tcatttgttt aaagaaatcc
    46321 ttatatagtg taagagtatt ttttattgtc aacattccat tcaaactttt aagtatactt
    46381 tttacctttg gtaaatcttc ttccataata ttatctgatt caaatttata atgaatgtct
    46441 tcagctacct tctctaatct cttcttaact ggatatctgt tatatgcctc aaatctactc
    46501 tgtatccagt cacttttagc tgtaaaactt ccaaaagtgt agtcttttgg aataaaaatc
    46561 ttatttggca tttgttttat ataatcatca ataagtttta caaaatcaag tgtagattta
    46621 aaacgcactc tttcagacca tttagcatca tttatttcta atgggtcttt ctcactttca
    46681 aaattaataa ctctatctaa ttgtacctct gctatatttt caaaacttaa ttcacataat
    46741 ggctcttctc caagctcagg aaggacattg gaaatatagt ctccaaagac tttattaggt
    46801 gataggataa ttacattatt tgctgatatt ttatctttaa atcgataaag aagaaaagca
    46861 attctgtgta atgctataga tgttttccca gaaccagcta ccccttgaat aatcagtgta
    46921 tcagctttat catttctaat aatttgattt tgttcctttt gtattgttga aatgattgac
    46981 ttcatttttt catctgaagt gtgactaagt tctttttgaa gaatatcatc ttgtatatta
    47041 atggaacttt ctagtgcata ttctaatttt ccatttttaa ttttaaattg tcgttttctt
    47101 gttatttctc catccaccca tcctattgga gcatcatatc cagcttttcc gatttcataa
    47161 tcataaaaca tactggcaat tggagaacgc cagtcaagta ttataagctc atcttcataa
    47221 tcaaatgcaa atctaccaat ataatactta gattcatcat ccatatcttt caacctaaaa
    47281 tctattctag caaagtatgg agaatctttg attttattaa tcttatctcg aacttttacc
    47341 ataaaagccc catagttatc tatttgtttt agtccttgtt cattttgaaa catttcatgt
    47401 gggtctattt caccacgatt ttttaccata tacattttag catccatata atccttatca
    47461 agcttgtcaa caagattttc agctttctta aaagcattgt ctagcttgtt atttatctct
    47521 attagatgtg caatctcatc tggaaaaaaa gttaataact ctgcatcata ttcacacata
    47581 ttatgtttag aattagatgc aataatatcg caatttacta cttgtttatt ttcagacatt
    47641 taaatcctcc tttgttaatt aagtacttac actgaatcat tatacttaat tgtgttttgg
    47701 agtttttaat cattcttgct atttaactta aaggaagaat aaatttttga gtaggatatc
    47761 caaattccac aagcaattct gcttctgcaa agcccaactt ctttatcata tctctatgtc
    47821 ctgtatcagc tttgtcaccc tctcgaaagg tagtaattga aatatctgta tctatcaaga
    47881 aatcttcaat aactttcttc aaaaacactt gtgcaatgtc tgaattacga tactgtggat
    47941 gtattccaaa aaaatcaata cttctagttt ctctattaaa caccatattt gcaatagcga
    48001 cattattaag ttttaatatt aaagctcttt cttctaaaat atactgttct aattgatata
    48061 tatattcttc ctcttgcaaa ttgggaaatc catctatgac taatctcact aaattcatcc
    48121 ataatggtat gtcagacata cttgcaagtt taatttcttt ttccaactct agtacattct
    48181 ttgaaaatag gttatctttt ttcaaaacaa atcttagctg caaaggataa aattcttgtt
    48241 ctttcctata tttatttgga gattttttat acatagcttt aaatatagaa gtgaatgcct
    48301 gttgactttc atatccagct atcaatgcaa tttcaataat tggtttcttt gaaaatacca
    48361 ataattttgc tgcttctgtc aactttctcc gctttatgta atcatgcatt gttaggccaa
    48421 cagaagttgt gaaagttcta tgcaggtgat attttgagta atgaacagca ttagcaacta
    48481 tatctaaatc cagcttttct gataaatgct cctctatata gtcaatagca gttatgacat
    48541 taattacttt gtaagacatt gcagcttccc tccttttatc catgcaacat tatatcaatt
    48601 attacttagt tatatttaat gaatcttgct attttataaa caaatttgtt gtttaagttt
    48661 tcaattcata aatttaatat attacttttt actttctgat taaagactct aatcctttaa
    48721 ttttcaataa cttttcattt agtaaatcta aaaagttatt tattattttc ttcttattag
    48781 attccttaat taaatgataa atatttatat tatacgacta aaaacagctt tttacaatta
    48841 tttaatatga ttacaaactt accctatctt aaagtttaat ggggtcctgt tatgaatctt
    48901 tttattttaa tttaagctct tcatcagttt attttatata ttgatgtatt tgatttcata
    48961 caaaaaagag tgcttcatga actaaaaagt tcatttttaa acactctctt ttatgtaaat
    49021 aaatttctat tctattttga aatattataa tttgacttat aatctattaa taccaaaaat
    49081 aataaattgt acgtctaaga atagattcta attagttgct cagtagtaaa atctgttttt
    49141 aactaatcct gctttgtatg ttgaacatat tctataattg taccatctgg atgtctcaat
    49201 gtcatattat atcctgtagg aacttttttc ttatctctta ctacaactgc tccattatcc
    49261 aatagataac ttctccattc atcaacagaa tcaaccataa aagtagattt tgtttctata
    49321 aatggtttaa gagcatcatc agaaccagat aatagtaaaa catttcctat ttgcgctaat
    49381 tccaacccaa cttctttata ggaaaaacgc aaagaacatt tttcttttaa aagattctca
    49441 taaaaaggta tggctttttc tatatcttga atataaaaac gtgttaatat atcatatatt
    49501 ttcatatttt ccccctatat atctatataa tattacctct aaattatacc actctacaaa
    49561 ttatataaca ataaattaag catagccttt tgagcatctt aagtagacct taacaatata
    49621 ttggggttac attagaatac ataaattaaa tcaccttgat attatttagg ataaaattta
    49681 tgtagtagca gtaaatgttc ttctaattat caactaacaa acgataatct ttaactttct
    49741 atataatggg taacatcatt ttcgtaagat attcactagc tggacgattg atatcattta
    49801 aatactggta atatatatcg ccatgaagtt tatatccatt gctatttgcc catgaaaata
    49861 agtcttctaa agtagggtct tgtaattcat aaagtcctaa atcaattgca gttattattt
    49921 tctgtgcagg aacaatcttt tgtagaatct catttttccc atctatataa gtggctacag
    49981 gaaaccctac ttcaacatcc aattttacta aatccatgtt atgaaaacat acaatcggtg
    50041 ctcctgaaga taaaattcct ctctcctcca gatatttaga aattttttca aaactttgtt
    50101 ttgaaaactc tgaaaattct accataaagt caattgtttt tcgtattgcc aaaaaacagt
    50161 actcagattg ttcagctatt gttatattgg atattctagt cattttattc ctccattact
    50221 ttattttcat ttctaatctt tttataccaa attatacgaa aattcactgt gtaactaatt
    50281 atttcatatg aatataaaac ataacctcct tccatttctt atatcgaaag tatagcaaaa
    50341 ataatctcat cattcttgtc acacagtgct cttataatca gaacatactt aaaagacttg
    50401 cctaattttt aggcaagtct aaaagaaatc tttttctata aaaaatttat acaaacaatt
    50461 aaaatatcaa aatatagact aatataaaac tataaattta ctaatctgaa cctcccatgg
    50521 ctaaagtcac aagattactg atttatagaa tttcgcacac taaaatatgt cttactaatt
    50581 ccccacaggc taccccccta gttcctacgt tcttacataa gttatctatt atcaaacatc
    50641 aaaatttaaa caagtttcag gttcatcttc tattcttgct attttaaact tacatgcaat
    50701 taatactaaa gatactattg gatttataat atttaataaa gcgtatggac catatgttag
    50761 tgcagataca cctaatgttg ctgttatata cgccccgcat gtattccatg gtactaatgg
    50821 agaagttaaa gttccactat cttctaaagc tctcgacaac atcttaggat gtatacctct
    50881 ctttttatat tccttattat acattcttcc tggtataact atagatagat attgttcacc
    50941 tgctattata ttggtaattg tacaagttac catagtacat aatactgttc caaatacacc
    51001 ctttgctaat cttagtaaag aagatgctac tacttcaagc atacctgttt tttctaaaat
    51061 acctccaaga gataaagcgc atattgttaa ggatacagtg gacatcatat tcataatccc
    51121 acctttagat aataaagcat ctacctcctg tatttgtgtt gacgaaacat atccattttg
    51181 tgctgcatct aatatcgtat tcatagattc tccttgaaaa actactgcaa acaatactcc
    51241 caatacagca cctactataa gccctggtat agcaggaact ttaaatacaa ccaatccaat
    51301 tactattgct ggagctaata ataatactgg tgataaagta ttaaatgtag aatctaatgt
    51361 agacctaatt aattctattt gttttatatc tagtgcttga cctgaatact tcattccaat
    51421 tacaccataa attactaaac aaattaataa tgatggtata gtcgaatata gcatatattt
    51481 tatatgtgta aacaaatctg ttcctgccat tgctggagct aagttagtcg tttcagataa
    51541 aggtgatagt ttatctccaa agtaagctcc agatattata gaacctacga ttaatccaat
    51601 tggcatacct aaactttgac caattcctaa aagagctact cctactgtac ccattgtgct
    51661 ccaagatgaa cctgtcgcaa gagatactat tgcacagact aatgttgatg ctactaaaaa
    51721 aatatttggt gataaaattt ttagtcccca gtaaatcata catggaacta ctccacttac
    51781 tatccaagtt cctataagta cgcctattat aattatgatt aagattgctt gcattgccat
    51841 ctttatacta ttaaacatac ttaattctaa ctctttccat ttaaatccaa gtctatatac
    51901 agccacaagt cctgctactg ctgctgctcc tattagtggt acatgaggtg atgaaccata
    51961 cactaatact cctagactta aaaataatat caaacaagta attggtatca ttgcatcaaa
    52021 cagacttgct tttctacatt ctcgctcttt cataaaatag tttcctccta attatttaaa
    52081 cactacacta taattttttt tgaaacatat ttgctcgtac tagatgacat gataccccca
    52141 taagttaata aaaattctaa attgtcacct aatttataat catattttgt gttacttaca
    52201 tctagtatca tgtggtctga acttgcccct aatattttta tatcagtgtc tattggaatt
    52261 aaagagtcaa tatttatatc ttgtttacct atagctatga ttgccctttt taagattccc
    52321 ttatcttcat aaacaggttt atttctaaag gcatcaactc ctatttctcc aataggaact
    52381 gatggttttt ctttacattc aactacttgg catattaatt taaatgcatc ttgatatgtt
    52441 ccatttatat tttcaccgta tgccgtttct ctaccaagta gaatggactc gcctattctc
    52501 aaattagtaa taccttctgg catattatta tttatcaaca gatgaataga acttgaatta
    52561 cctccagata ctatttctag atttattcca aatttttctt ccattctttt tgctatactt
    52621 accagtctgc ctatattttc cctagatggt ataatagctc cataacaggt taagtttgta
    52681 gctataccaa ttatttcaat attttttaat cttactatct ctcttacatt attaaaaaag
    52741 tcttcttcat aaaagtatcc ttctcgtaaa tcgcctaagt caaacataag aactatctta
    52801 tgaataatat ttttactttc acataattca tttaatttct ttattgttcc taattctgag
    52861 ttaaaactaa tatcgcaata attgataact tcttctaatt cacttaacat tgggattcta
    52921 atcaatatct taggtagatt tatatcttgt aaatttttta agttttgtat tcttgaatct
    52981 gctatatggt ctattccttc actacatatt tcttctacta tttctttttg tgcacaaaat
    53041 gatttagtaa caaaagatat cttaatattt ctttcatgac acatctgcga aattaatttt
    53101 gcattatgct ttaacttttc tatatctatt tctaacctag gatacataat ctttcctcct
    53161 caaaattcaa actttttaac aggagcttaa gagatgcttc ttcatatttt ttagataaaa
    53221 cacccataga tgccataata tagtcattat ctattaatat ctttgttttt tcctttggaa
    53281 atagtaattc attattttta ttgtatttga gcatattatc taatatttca attctactag
    53341 ggagcctagt aagtgctccc cctgtaccaa ctatatatct tacttctgtt aaatctttcc
    53401 cttctgcaat tttgactttt ccagttgttc catagatatt tcttattttt cctgaatgtc
    53461 ttagcatagc tttaacaaca gcttcttttg tcaatctttc aacaaataat atttcttctt
    53521 ttgattttgg tattggagga taattctcta ttactgcatc aatatctata gatagttcac
    53581 tttgtaagtt ttctttacct ataacttcta ctatattctt catatttaca tatacaccta
    53641 aatccccctc aacacttctc tttgcagtag gttcaggatt aaccaaaatt ttattaatat
    53701 agtcacttcc atctgtaaca gaatgtacat cagtagttgc tccacctaca tccagagtta
    53761 ataaatctcc aatattttta tataaaagtt tagaagcttc cattacagct cctggggttg
    53821 gagtaatatt ttcattaacc atttctttaa tgtatttcat tcctggtgct gtagttatgt
    53881 gttcttcaaa tacactttga atgattcttc ttgtaggttc tatattcaat aaatctattt
    53941 tagggtatac attttcagtt atataaagct tataattagt atcttcaaat attaacctta
    54001 tttcctcttg attttcaaca ttaccagcat atataactgg tatgcctaga ttcatagaag
    54061 ctatcatttc tgcattataa atagctgtat ctctttctcc ataatcgact ccaccagcta
    54121 ttaaaattat gtttgggttt atttctttta ttttatttaa atcagttctt ttaattctac
    54181 ctgatgttat ttgacgaata acagctccag cccctaaagc tgcttcttta gctgctttta
    54241 ctgtcatatc atagactaat ccatgaacag tcatttttaa tccaccagct gcactagaag
    54301 ttgcaaacat atcattatac tctatatcat ctacagataa tttattagct aaatctttaa
    54361 tagcacctga taagccattt cttacatcgc caccttcaaa cactgttgtt ggagcttgac
    54421 cttgacccaa aaatatagga ttgttagtat taatatcgtg gaaagcgttt accactgtag
    54481 tagtacttcc aatttcagct accaacacat ctatttttaa agaaaccttg ctattttcca
    54541 ttatttatta ctcatttctc ttcttttttc aactaagaaa gtggctacat gtatgccttt
    54601 actgtttctt ccaaatccag catctatgcc ttgttcaaca gctatttcag gtgttacttg
    54661 tgttcctcca cacgcaatca ttactttatc tcttatacct ttttctacac aataatcgtg
    54721 taattttttc atgtttttat agtgaatatc atcatgactt attattgtag atgcaagtat
    54781 tgcatctgca ttcagttcaa tcgctgcatc taccaatttt tctacaggta cagaagtccc
    54841 tagatagtgg cattctatgc catatttttc aatacctcca tgttttatat ctattatttc
    54901 tcttaaacca actgaatgtt catcttctcc aaccgtagct gaaactattt tcataggttt
    54961 ttcttctata tctgctctta tttcttcatc agttagtacc ttaggttctt ctggtattac
    55021 taaattgttt aaatctatat caaacggaac tcttcctttc acttctattc tagtcccttc
    55081 agattcatgc ataacttctc tacttatgac ctcaatttct tgtaagttca tttttttgac
    55141 aaattccaaa gctgcaaact ctgctgttct tttatcagta ggaagcatca ttgttatcat
    55201 tataattcca tctgcacacc attccatttc aggcttaatt ttagttccat ttctgtactc
    55261 atatgattct tttagtctat tttctacatt atcaaaatcg tcaagctcat caatatatat
    55321 tattttgtct ggattttcaa atgtacatcc atctattaac attgatggat tatctatagc
    55381 attttcatca tattgagcta cattattgta tccaaagtga gcagttacag gtgctaaata
    55441 atcttcttct ctttcataaa cagtaccttc accaactcca ccttttattt ttctagatat
    55501 tccatcacca tttctttctg gataatttcc agaatcaaca aaaaatcctt gttccacaga
    55561 ttcaaaatat ccaccaactt cagatatttc ttccatatat aatacagctc tttctttaag
    55621 ttctcttgct ttgtctctta attcaccgac atctttaagt tctatcatat ccataagccc
    55681 atccatacca actaaagctt gctttgctgt atcacatgct tctatattgt atatatgcca
    55741 aggtacattt ctaccttcat caggtgttat agttgattgt atgtcagctc tagttaatac
    55801 tgatgttaat aagtttaata catgagtaac tgttgcttct ctagttgatg attccatata
    55861 tttagtattc atttgagctc tcatcttata atcactaaat aactctctaa gtgcaacagc
    55921 gtaaggtaag tctattttta aacatggtgc tggaggtgct gttggtggaa ctgttgataa
    55981 gcatatatta cttttatcta tacctatttt ttctgagaat atagagttta aagcgtgttg
    56041 aaccataagt tcaggcatta ccttccatgc ttctctagca gtagcattag cattatgtgc
    56101 tccatcaatt tgagctatat tggcaaaagc cattattttc tttgcctcac aagcatctac
    56161 aaaagacctt accatgttta tattcctata cagaacatta tattgtgggt cttgatgtgc
    56221 tccattaact ccttcctcag caaacattac tgctacttca ggaccagcaa caccacttac
    56281 atatgaatga taattaatag gtctaccaac ttcttcttct ataaaatcaa gagccttcct
    56341 ttgagctctt acttgttttc ttgttattgg aactccacct ataccttgtg gggttccttc
    56401 tataagccca tcaaaatgtg attgtccagc agttctgata accattagat ggtcagctcc
    56461 atgatgagct gccattctca ttcttcttat atcatcctca aatctaccag aagctatttc
    56521 agtagtaata actggacttg gttgagggtc tatattattg aaatacttag atgatggtag
    56581 ccctacactc ttctttaaag gttttgtaca gtctttatat ttaaaaggtc ccatttcaag
    56641 attttccaag tgttctctcc aaacccaacc tcttcgtttt ggtttatatt tatccaaatc
    56701 ttttaatata tagtctatat ccaatttttt attttccttt aacatgattc taccccttct
    56761 ctaaataaac atgtaacttc atcccaatat tcacccttaa ctaataacaa acctgcttct
    56821 ctaaccgtta tatttttaga tttagccaat ttataaacta catgccctgc tcctttacct
    56881 ataagacctc tttccataac cccttctaaa ataggtttta cttcaagaga agaaaatccc
    56941 attctcaaca agacagacct ttctattgat ggtgttgtat tctttttacc aagttctatc
    57001 attggttcta ctatctgggt agctaaactc caaaaccttt cttttaactc atcatcactc
    57061 agttcttgaa gatgttttct tctaacttca aaatcatcct ctcttttttt catgaacatt
    57121 cctccattcg tattgattta atcgaatttt ctgaattttt attttatatt aaattaaaaa
    57181 acaataatta ctataaatta tttaatacat caacaacaaa atctcttgaa ctttttactt
    57241 cttccattaa aaattctata tctcgattac ttagcttatc aatattatca atgtgattat
    57301 tagatataca attttttata aatgactttc ttattttatt catatcaata tcaacacatt
    57361 tcaataattc tggtctttct ggtaatatta agtttactcc tgcaatttct tctcttggat
    57421 ttccaaattt tatatctatt ccattttctc ttgcaaaggt aagttgtgga catatatgtt
    57481 tacctgcacc cgtatattct gtttcttgta ccactatagc tttatcctca tctagctctt
    57541 gagctaaaga gaatgctgct gctaaagatg tatttcctgc tggacctctt tctatacctt
    57601 ctagctgtgc caataattca gttgtataaa aaacttctcc ttgttttact aatacatatc
    57661 tatccatata tctaagtggt ctagctgcag acctaggtac atctgaccta tcaggccatg
    57721 tacagtatgg tatcccaaaa cctgtatgtc ctgttgtaaa tgatttttta ttaaattgat
    57781 tatcactagc catgtgtaat ccttttaaat ctacacttgc tcctactact ttagtattaa
    57841 tagaaccagc ttttatcaat cctcttgcag ttccagtaag attaccacca cctgcatttg
    57901 tacaaacaac tatatcaggg tcttttttat acttttctct aaattggata gctaactcat
    57961 aacctaaagt ttctacacct gcaattccaa atggtgaata taatgatgcg ttaaaatagc
    58021 ctgtttcttc taataaaact aagaacgtat aaaataactc tggtccaaca gttaactgaa
    58081 caacttctgc tccatatgcc tcacattttc gcgctttttc tatgatttca ggttgaccta
    58141 caccattaga atcataacat tcttgaacta ttatacattt taatccctgc attgccgctt
    58201 gtgatgctac agctgcacca tagttaccac tggtagctgc tattacacct ttatatccca
    58261 tttgtttggc atgatatact gcagtagctg ctcttcttgc tttaaaacta ccagatgcat
    58321 tcattgcttc atcttttaca aatattctag ctcctttacc ttctttagca cattttctag
    58381 ctaatttagt caaattttta agctctatta aaggagtatt tccaacagca tactgtgatt
    58441 gaattgcttc aatatcttgt aatgtatatc ctgtttcact catcattttc tcataatcaa
    58501 accctattcc ctcttgttca aaagaagaat aatccaatcc gattgcattt ttcattattt
    58561 cgttatttct tcccataaca gcttgataac tcatatcttt cattgatgtt gaattattca
    58621 ttatatacac ctccaaataa tatttcttta gcttggattc ctatttggag taactctggt
    58681 atacatttac cataatcatg agtataatat ggatttattt taagtaaatt accttttaca
    58741 agtctgcctg taattgtttt aacttctact aagtcgccta tagaagcatc actttgtata
    58801 aaacctttaa cccacatttc taaggatact ttcttagtat cctctggaac ttgtggagct
    58861 ctttcttctg gagtcaatac tatattgtgt ataataaccc aatcattaat tttagcatcc
    58921 attttttcat ccccttatta attgactatt gccttactaa ttaatttact gttattttaa
    58981 attaaacatt attttaccaa ttagctaact gttatcttac taattagcta gttgctatct
    59041 tactaattag cctttgattc ttcttccaat tcaacttcta tctgatttct tatatctccc
    59101 attattgctc taggtactgg tatatccaac atagttttaa gacctggtct agcatttatt
    59161 attaatggaa tagagtttac tatcattgca taagtaccta ctcctccagg tatttcagga
    59221 tttatttgta aatcaatgtt tggtatacct tttattgata catagtctcc tgttttaata
    59281 ccttctgctt ctggtattat ctgttgtgga tgctccattt caattaaaac ttctccattt
    59341 acatatccat atccacattg tctacaacca gcaacatttc cagccaatgc ctctccgtac
    59401 ttagattttc tatctactgt agtcattatt ggctcttttg tttgttctat cttatctaat
    59461 ttccaaccta atccatcagc tatcatattt attgattcta caaaacctac atgacctgct
    59521 atagtcttat cttctacacc ttttataaat tcttcccttg ttactccaac accttgttcc
    59581 accataacag actttccaaa tggagataaa tcatttactc tcttagcttt tatactagta
    59641 acttcttcac aagtacctga aagtgccaat actagtaaat ctaatacaaa tcctggattt
    59701 attcctgttc caagtatact tactccattt tcctttgcaa cttcatccat ttttttagct
    59761 aaatctgcat catcagcttg tggatatgcc atttgttctg ctgttgatat aacatttatt
    59821 tttctattta ataagaaatt tattttatca aaagcctttt tagtaaatga atcagttgct
    59881 aacaaaacaa catctacact tttttctcta aaaacttctt cataattttt attgataata
    59941 acttctggtc tttctcctct ttcaattcct aaaacatcat acatactttt ccctgaagta
    60001 cttctactac aaacagatac tatttctata ccttctttct ttagaatcat attagccata
    60061 cctattccca tagctccaaa tccccatatt cctactctta cttttctcat atatacaccc
    60121 tcctaaaaat ttatatattc tcaataatta gttaaagcat aaatcgtgcc aactttaaaa
    60181 ttaaagtaaa tatttattgg aacgttgaaa ctataattat tttattatgt attttcataa
    60241 ataaaaaata atatttctta tgagaaataa aaaaaagagc aaaatatttt gctcttttat
    60301 aataattttg ctcctctatt ttgctttttt attttacttt gattttgttt ttttatcttg
    60361 cttatttata atattttgat attatatttt tttattttat actgtaaatt ttgcctattc
    60421 atacctaaaa ttgatgcagc tttagttata ttgtaagatg ccttttctaa tgattcatat
    60481 atatactttt cttctatctc actgataatc ttactaagtg ttttgtcctc caaatcgttt
    60541 aaagaataat tgttactctc tttattctct ttaagagtgt aatagtcgtt tagattaatc
    60601 atacttgttt ctatttgtac ctcatcttcc atcatactca tagtacaata aattatgttt
    60661 tctagctctc ttatgttacc ttgccaatcg taatttttta aaattttaat agcttcatct
    60721 gaaattccta ccactttttt atttaacttt ttattatact ttgaaataaa ttttttgcac
    60781 aatagaggta tgtcctcttt tctatctcta agaggaggaa tttttatatg aattatgtta
    60841 agcctataat ataaatcttt tcgcatttta tgctcgttta aaattacctt tggactttca
    60901 tttgtcgttg caataattct tacatctata ggtatatcct tagttcctcc aacccttctt
    60961 acataacctt cttgtaaaac ccttaatagt ttagcctgta acataatagg catggaattt
    61021 atctcatcca ataacaatgt ccctttgtta gcttgttcaa ataagcctgg cctatctatt
    61081 gctcctgtaa atcctccctt gctagttcca aaaagaatac cttcgaatag tgattccggc
    61141 aaagctgcac aattttgtgc tataaatgga tagtttctcc tattacttcc atagtgaatg
    61201 gactggctaa ataactcttt acccgttcca gtttcaccgt atataaatac tgaagcatca
    61261 gacttgcaag ctttcttaga tttagctatt gcttctttaa tcaaattact ttcccctaaa
    61321 atatcatcaa attcataacc ctttttttgt ctaatactaa ctttattgac taaatttttt
    61381 ttataatttc tatttagtaa attttctatc gttgtcatat tctttgatat ttcaagtgcc
    61441 cctacaaact cgcctttaat atcaattggt attgtagtat taattgctgt tatctctttt
    61501 ccatttttat ccagatattt ttgaatattg tctttgataa cttccctttt ttccaaaact
    61561 tctaatagtg tactcctgtt tattttcatt ttgttgacta tatcaaaaaa tgaattggtt
    61621 aaaatatcgt gtctgttcat tttttccatt ttttgtacag aattattgta gattatagtt
    61681 ttcccgtctt tattaacaaa atgtacccct tcatcaatat tttccaatat ttcattcatt
    61741 attttaaaaa atatctcttg gttcaactat atcaccccag tacctaaata tataaaaacc
    61801 caaaaaatta ataaaatgtt gctattgtat ttatcttatc atatatttat gatatttttt
    61861 atgtacatta tattaatata tttttaagta tatttacaat tttatttata tatctttcat
    61921 tatggttata tacaatattt gaattgtcat taaatatgcc aatagtttta tatattattg
    61981 attttattaa aaaaagagat atacaaacta ataagttgta atctctcaaa tataaaaaaa
    62041 caataaatta
//
LOCUS       DAEPIN010000014        62001 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.014, whole genome shotgun sequence.
ACCESSION   DAEPIN010000014 DAEPIN010000000
VERSION     DAEPIN010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 62001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62001
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.014"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            150..734
                     /locus_tag="KR250_001063"
     CDS             150..734
                     /locus_tag="KR250_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HBH0067027.1"
                     /translation="MLMNTHFIMAKSILDNIDENKTFFISEKNFIYGNIKPDAFSKYK
                     LKKHYLDESFNMIVSKINYLCSLNIDSLSKYFSVSRLSQELGVICHFLCDFFCVAHSE
                     RWEFKHSLNKHVTYERELGAFAKDVDLSKIKCGTINSSFEKFFTKLYSDYKKKTDYMN
                     DLKFSAYTCNSVINYILDSILSNTIKSYHIANCG"
     gene            complement(838..1617)
                     /locus_tag="KR250_001064"
     CDS             complement(838..1617)
                     /locus_tag="KR250_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBH0067028.1"
                     /translation="MIKHIFCDLDGTLYENGTITKEDIVAIEEIEKKGVQFNVATGRI
                     FKQAHNIIKDSLDMNGYYVCENGSFIYDKDYNVIFKRTIDDNLVKKVIDRFESSDAQL
                     YFKYKGDVIVGSDTTAFRHYSSDFIVDPDFEKRSSFDNLIGNIGVCSENLEELSRIEL
                     YLKSEFSEVLEIYFSGTYTLNIVPKSVSKRGSIEHVIKTLNVSPDEVATIGDSPNDIC
                     MLEGFKYSFAMSKAREDVKQSANYVVDSVKSAIDVIMEINS"
     gene            complement(1675..2931)
                     /locus_tag="KR250_001065"
     CDS             complement(1675..2931)
                     /locus_tag="KR250_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011591286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HBH0067029.1"
                     /translation="MKAEIISVGTEILLGDIVNTNSQFLAKELASLGIEVYHQSTVGD
                     NKQRLLECFDESLKRSDFVITTGGLGPTGDDMTKETAAEYFGQKLELHKPSLEVLESF
                     FVKTGKKMAENNMKQVYFPKDAIVLKNNNGTAPGAILKKDGKFIIVLPGPPREMKAMF
                     NESVKPYLQQFTNEMLVSKTLRLYGIGESNLELEILDIINEQTNPTVALYAKELEVTI
                     RITAKAENEREAFKLIKPVEEKIKSRVGKYVYTEGDISISEGETALEDAVSKLLVEKN
                     LTIAVAESCTGGLVSSSLINYPGISSVFLEGCVTYSNDSKMKRLGVKRETLEEFGAVS
                     EQTAIEMAEGVAKGLKANIGISTTGVAGPGGGTKEKPVGLVYTAIYINGKTIVKKNIF
                     NGDRRKIRLRATRDLLNELRIQLEKL"
     gene            3189..4088
                     /locus_tag="KR250_001066"
     CDS             3189..4088
                     /locus_tag="KR250_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HBH0067030.1"
                     /translation="MKIAVIMGGISSEREVSLNSGKEIYNNLDKNKYEVVKVIIDDKK
                     DIFTKIPEDIDFAILALHGKFGEDGCIQSILETMDIPYSGCGPLCSGMCMDKNITKKM
                     LRDSNLPTAPWVLVKSVDEIDYDEIDNIGYPVFIKPNSGGSSVATFFIHSKDEVEEAV
                     RKGLEVDEFVMIEKYIPGGEYTSFILNGEVFPTISIKSDSGFFDYEAKYSVEKGAKEE
                     VVYLDEELQKRVNEISETCWKIFNCKAYVRVDMIISEGIPYVLELNTLPGMTQTSLIP
                     RSAAARGIKYSELLDKLIEYSLN"
     gene            4112..5560
                     /gene="ddlR"
                     /locus_tag="KR250_001067"
     CDS             4112..5560
                     /gene="ddlR"
                     /locus_tag="KR250_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator DdlR"
                     /protein_id="HBH0067031.1"
                     /translation="MIFSNLKLNDNEPIYIQLKNYISDMISKGLIPDNSKLPSTRELS
                     QLLQVSRNSVVLTYEELKSEGLIYSISGKGTFVKSKNKSSNTTWSLNWDCLENTYSKK
                     ANELDIIKSEIPWSSDLISFKSISPDGDLFDIEELKKSFLNRISLEGHKLLNYGYAQG
                     YKPLIDYLLEYMTNKGADISNKDILITNGFTEGFDIIMSSFTQEKDYIICENPTHNTA
                     IKIMKSHNLNILGVDINEDGLDFNMLEDNLVKYKNKIKFAYITPSYHNPTGIVMTPEN
                     RYKFYNLMKKYNIAIIEDGFNEELLYNSSHIFPICSLDNMNNGVVYIGSFSKILFPGM
                     RIGWIFADKKVINKLESVKRCRNIHVSFLDQGILFDYLSNGGFEKYIKKIRKFYGDKF
                     NFAYKCIKKYIKTEYILGDGGLHIFIKLKNIDTRVLLEKCYEKNVIFMPGDLFFTDNS
                     GFDTLRLGFSRLSFEDIEIGIKIIGETINEMF"
     gene            5619..6425
                     /locus_tag="KR250_001068"
     CDS             5619..6425
                     /locus_tag="KR250_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBH0067032.1"
                     /translation="MNLILGDNMSKKSKRRKINKLKLTIVITVLFLALLSIYEILFSN
                     PNKKGNIKSQETMQNKNNTTDDSKNNQSSTKKQEDNNIKPTFSYSSIPDNIKNKMIGK
                     SMPTDEPISFDSLSYLKLTYYGFDEKTHQGEMIVNSELAPEVVDIFKELYEKKYPIEK
                     IKLIDDYDAVDEKSMSDNNTSSFCYRTIAGTNVVSNHGKGRAIDINPLQNPQVSGNDV
                     TPKVSTVYADRSSTKFGMIKKGDDCYNAFVSRGWSWGGYWKNPDYQHFEK"
     gene            6451..8370
                     /locus_tag="KR250_001069"
     CDS             6451..8370
                     /locus_tag="KR250_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_071223118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBH0067033.1"
                     /translation="MNLMTLENISKSYSEKKLLENISLGINEGEKIGLIGVNGTGKST
                     LLKIIAGVEESETGNITKTNGIRIEYLPQNPTYNENSTVLEQVFNGTSEELKLLGEYQ
                     EILEKLNSNFTEELNKKLLSVHEKIDALNLWDLESEAKSVLTKLGINDFNQKIAELSG
                     GQRKRVSLASALITPCEFLILDEPTNHLDNDTIDWLEEYLNSRKGSLLMITHDRYFLD
                     RVTNRIIELDKGRLFSYDGNYSIFLEKKMERLSLEASIEDKRQNLIRTELAWVRRGAQ
                     ARSTKQKARLQRFDELINRDSFKPDENLDISVASSRLGNKIIEIHNISKSFEENTVID
                     NLEYTLTRTDRIGIIGKNGMGKSTLINILNGEIEPDSGYISIGETVKIGCFSQDDSHM
                     NIDMRAIDYVKEASDYIETSDGTRITASQMCERFLFNGTMQYTLIGKLSGGERRRLHL
                     LKILMTAPNVLLLDEPTNDLDIETLKILEEYLDEFKGVVVTVSHDRYFLDRICNKIFA
                     YEGNGKIHIYTGNYSDYLIYREIQGIEFEESKKESVKKDDSTTLKKEKPKNDKKLKFS
                     YNEQREFENIDSDIEKLESKIATLDESTAKFSTDFTKLQEILDEKSKLEKELEYKYER
                     WEYLNNLADEIANNK"
     gene            complement(8612..8959)
                     /locus_tag="KR250_001070"
     CDS             complement(8612..8959)
                     /locus_tag="KR250_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HBH0067034.1"
                     /translation="MSKSNDVLDIVFKITDGLEYEVAENRVVTILEKQDHKIQKFFRK
                     LKARIPKYKKIELDEYSSFVFLQIDGQRTVREIGKNLEAKYGEESHPLYERLLVFLNH
                     IEVNCHYIERLDI"
     gene            complement(8946..10874)
                     /locus_tag="KR250_001071"
     CDS             complement(8946..10874)
                     /locus_tag="KR250_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HBH0067035.1"
                     /translation="MNKKLPKGAYGEVSGKDYVPYITDKSRTGGNVAVLIIGIILAAI
                     FAASTTYSGMKAGLTVAAGIPGAIIGSAFVGAFARSKGILGKNLIQGMSSGGESVASG
                     FIFVLPAVILIGSQITFFEGLAVGVGGVLFGIGIAAIVHNYLIVEEHGKLMYPESMAI
                     SETLVASEAGGDSIKYMGIGFVISGFITVLTGSFLNVANNVMSLVGSKFYKWKFDIEV
                     NPLLLGIGFIVGLEVSLTMFAGSILSNFGIAPLIGYFTDMAKDGAMVWNNPAMPLNQM
                     DVGAISSSYVKYIGAGMMLCGGIIGAIKLIPTIIASIKETLKAKSSAGEGEEGSSIQM
                     ILLLGGVVIGFLAAFLISGNIVMAIIGAIISLLLSLLFVIVAGRLTGTIGTSNLPVSG
                     MTIASLVIVTLVFVIMGWTDLEANKSLLLFGSFIVVAIAIAGGYTQSQKVTYIIGGSK
                     NEMQRYFTIASIVGVIVVVGVILLLSDQLRATGDNVQFALPQANLMSTLTSGIMSGSL
                     PWVMIIVGVFMAIVLYALNLPIMTIAIGFYLPIATTSIILVGALIRLFVELVSKTEKE
                     KEVKVSNGISLSSGLVAGGSIIGLIGIILQVTGVVTPKVPSGFAATNSMAIALLVVLV
                     VLTALPIVLSKVKNNEQE"
     gene            complement(11285..13474)
                     /locus_tag="KR250_001072"
     CDS             complement(11285..13474)
                     /locus_tag="KR250_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HBH0067036.1"
                     /translation="MYEVNLERAMNYWMDLLDDYTNIAKITNDFFYKDCSDEVVDEVF
                     KMNIEKHERLIQYSKVNNISIDSIIETIYGLILQKYIYEDDVVFGKTMNIIPIRVRVR
                     QSEKFIDVVRKLKRQWSRSLEYGYYLLSDIENKNHLNTKLINTIFIAKSFDLSYDYDI
                     EKKGYDLSVIVYTKRALKIKIKYKPSIYKRETIRRILNQFDYIIEQIIEDNDILIEDL
                     EFVCKEEEKVLLEDFNNNQYEIPYDKSYVDLFREQVKVTPSNIAARDENRKLTYKELD
                     EVTDKLAGYLNHIGVESEDIVAVMLPRDINIIITAIGIMKSGGAFFPIDTSNPEERLN
                     YLLEDSNAKVVITTDELKSKVVNENTIVLDINDEEMFKLGYELTEKITSSNCAYTIST
                     SGSTGRPKTIAIEHKSLVNMCYYSVKSISATENDICGIYLSFSFDAVMKQLFPYLLVG
                     ASIDIMPEEAKFDEYTVNEYCEENDITILALPTAFARLFIQNCNNNSLRVVQTGGERL
                     KGYRKRNYELYNEYGPTEFTVVSTSFHVDREYGKIPIGKPIFNTYAYVLDKKNKLCPI
                     GAPGELCLSGIQISRGYLNKKGLTEQVFVENPYKTCEHNKLMYRTGDLVRWLDDGNLD
                     YIGRMDNQVKIDEFRIELYEIENIINNITEIKSVVCISRTNDDGDMYICAYYVIDEED
                     SGKINERTIREYLNEHLPPYMIPTIIMRIDKIPVTPIGKVNKRALPE"
     gene            13805..14536
                     /locus_tag="KR250_001073"
     CDS             13805..14536
                     /locus_tag="KR250_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBH0067037.1"
                     /translation="MNIYAHRGFSGKYPENTILAFKKCLDMDIYGIELDVHRTKDGKI
                     VVIHDEKVDRTFNGHGFVKDFTLRKLKTLNSSFEGYQSNKECKIPTLEEVLILISPTD
                     LILNIELKTDKINYPNIEKDVLELILKYNMKNRVLISSFNSNSLKNFHKLDPSIKTGL
                     LCYLPINNVVNFAKFLGNSYLHPPLVLVNESLIELCHKNLLGVNVYTVNEEGDILHCL
                     KLNVDGIFTNYPDIASNLLHSKQYS"
     gene            14616..15251
                     /locus_tag="KR250_001074"
     CDS             14616..15251
                     /locus_tag="KR250_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBH0067038.1"
                     /translation="MDNLLNELEALKSNLDNLPIEKIENYDLSKTALFIIDVNNGFAR
                     QGALYSPRVESLIKPIEMFTKKISNKLNRVIAFTDSHTPKSIELLSYPVHCLENDVES
                     ELVDELKSIENLQILPKNSTNGFFALENLDFDNIDNIIIVGDCTDICIYQFAITLKSY
                     FNQHNIEKNIVVPMNLVDTYDIPNVHPAEILNLVFFNSMIQNGVNVLKEIR"
     gene            complement(15502..16095)
                     /locus_tag="KR250_001075"
     CDS             complement(15502..16095)
                     /locus_tag="KR250_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3867 domain-containing protein"
                     /protein_id="HBH0067039.1"
                     /translation="MSDDRIIDFNELKNKVKDSDVDKFEQYIYNLYFSVMDGKMSMAE
                     FSRKIFDYMRDNNISQEKFMKIQKQFMERYGMDTEEVEKQLRNFGIDPSTAGFMSNNT
                     SSKVSTEDLESFKKSAGFYEKYGEKIQPKSCITTFIKNDLNDINVIIDQEKIMLCSDR
                     KINLMDSELNEFLLEYKNMFNKKIKVVMCETTNKYDY"
     gene            complement(16166..17029)
                     /locus_tag="KR250_001076"
     CDS             complement(16166..17029)
                     /locus_tag="KR250_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HBH0067040.1"
                     /translation="MLGIIAPSGPLRNTSLEEIKFNLESYGYEVKFSESCSLNYKGYL
                     AGNDDIRARDIEDMFLDKDVDIIMCLRGGYGTTRILDKINYDIIKQNPKPFIGFSDIT
                     GLNLAFYKNCGLLPYHGIMAANVGKWGKFTYKSLVNALEFKDELYLENPKEEKIYTVC
                     EGKAEGIIMGGNLSLIIATMGTKYEIDAKDKILFIEEIGEPQYKLDRMLTQLYSSGKL
                     EECNGIIFGDFKDCIEENDLMELLIEFANKVNKPSIYNLQSGHCIPMITIPLGRMCEL
                     DATEKIVKLKK"
     gene            17192..18361
                     /locus_tag="KR250_001077"
     CDS             17192..18361
                     /locus_tag="KR250_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBH0067041.1"
                     /translation="MLNIKTLCHEINDWVINIRRDLHKTPELGLEEFQTKKKIIKYLN
                     EIGINYIEYKNHTGITAYINVSPNFETVAIRADIDALPITEELNYSYKSINIGKMHAC
                     GHDAHTAILLGTCNILFKLKDYLNVNVKFFFQPAEETIGGAQLMIEDGCLEKPNVKYI
                     FGLHVNPNINKNLIELKYNTLNASTNTLQLTVHGSKCHGAYPHQGIDAIVISAHIITA
                     LQTIVSRNTNPTDSVVISLGKIEGGIKENIVCDKVVIRGTLRTLTPETREFSKKRIRE
                     ICDFTCKTFGGSISVEIEEGYPALINSNHLVDYVKQNAVELFGEENIILKDSPTLGAE
                     DFSYFLRHCEGAFYHLGCANREKNITSPLHTSTFDIDEDCLITGVMLHVKNVLSF"
     gene            18462..18938
                     /locus_tag="KR250_001078"
     CDS             18462..18938
                     /locus_tag="KR250_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HBH0067042.1"
                     /translation="MKEILINPIGKICIQGEEVFIKLDKKYVPALNELDDFSHLNVFW
                     WADGFDNPELRSILETPKPYKSGPDVIGIFATRSPIRPNPIALTAVQIINIDHENGII
                     KIPYIDANDNSPVIDLKPYTPSVDRVENPSVPKWCSHWPKSVEKSGEFNWEDEIEF"
     gene            19504..20298
                     /locus_tag="KR250_001079"
     CDS             19504..20298
                     /locus_tag="KR250_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HBH0067043.1"
                     /translation="MKLYLSADIEGTCGIVNWDETKLECEFSQHYRTQMSKEITAACD
                     GATQSGVHEILVKDAHDSGRNINPNILPENVKILRGWTKDPLVMMAGIDESFDACIFT
                     GYHSGATCNGNPLSHTMDIDYDYFKINDEIASEFTINAYIAAYYNVPVAFLSGDEMLC
                     ESAKKLNPNIVTVPVSKGIGNGSISIHPNLALKKIEKGVKKALSGDLSRHLIKLPDKF
                     KIEIKFREHYKAFKASFYPNMKKIDSQTVLFETEDYYEFLRMLLFI"
     gene            20322..20765
                     /locus_tag="KR250_001080"
     CDS             20322..20765
                     /locus_tag="KR250_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory YrvL family protein"
                     /protein_id="HBH0067044.1"
                     /translation="MIKISNKRRIPLGEFISVFLFFAVFFGITFCIFTVIGIGFLSFL
                     GFEYKSLGAVLIFFLIYFCITTPIDFLCTTILDIFRYVNKLPYSIYKLCEFIIDFILT
                     FLAMNIIDTFMDSVTIPLSTEILFALLSYLLSECMDFFDRDKKKP"
     gene            complement(20854..22140)
                     /locus_tag="KR250_001081"
     CDS             complement(20854..22140)
                     /locus_tag="KR250_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBH0067045.1"
                     /translation="MEKVKVAVIGAGNRGTYAYAPYIYENSDVCEIVAVAEPKKGRRE
                     LFTQKYNLDSKNVFETLEDFFKHDKMADAVIIATNDDRHYDVAKLALEKGYHVLLEKP
                     MSNSLDGLVHIDDLCDKYKDKIFMICHVLRYSPFYNKLKEIVESKKLGELVSIQYNEN
                     IGYWHFAHSFTRGNWRNSNETSPLILAKSCHDMDILLYLVGSRCKKISAFGSLKHLNN
                     QNASGEMAQNCLQCLVEKKCPYSAKRIYLEKDRSINRAVHINPTEENLLNILKTSPYG
                     RCVYRCDNNVVDNMVNILQFENGVTATFNLCAFTKENGRTIKLMFSHGEVGGDLNKNE
                     IRIKEFGKNEEIVMNPSNQQNMVEEYDRNLIAEFIKLVSNKELEKGRVAAKEAIQSHV
                     MAFAAEYSRVSDEVVYIEEFFDSAKQMTKEIEETIF"
     gene            complement(22358..23248)
                     /locus_tag="KR250_001082"
     CDS             complement(22358..23248)
                     /locus_tag="KR250_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBH0067046.1"
                     /translation="MSSTIKSNRIKGIMFIIASALGFAMMSAFVKLAGDLPSFQKVFF
                     RNLVSAIIAFWLIIKHKGSLTGKKENLKVLLYRSIFGTLGVIFNYYAIDKLMLSDANM
                     LNKMSPFLVVIFCAIALKEKINVKQIGAIIIAFIGALFIIKPTFSVEVIPYLGGIAGA
                     VFAAMAYTCLRVLGDREDYYTIVFFFSVFSLVTVGPIAFAVYEPMTLMQLIYLLLGGV
                     FASLGQFGITLAYKYAPAKEISIFDYSNIIFSAILSIFLFNVYPDMLSVVGYLIVFSA
                     AFYMFLYNKKLDKLDKEKKK"
     gene            23540..23884
                     /locus_tag="KR250_001083"
     CDS             23540..23884
                     /locus_tag="KR250_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HBH0067047.1"
                     /translation="MEIKRYEGTGRMSRAVVHNNTVYLCGQTHAEGGVIEQTTEVLAK
                     IEDLLNKYGSDKQHLLSVTIYLRDMKDFEAMNSVWDAWVEKGFEPARACVEARLAREH
                     LLVEMSVVAATK"
     gene            complement(24043..26139)
                     /locus_tag="KR250_001084"
     CDS             complement(24043..26139)
                     /locus_tag="KR250_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HBH0067048.1"
                     /translation="MIKFKLYISGLYSGNVIFDGDLLIEKLNPFTNKIESLKPISKEE
                     NTYYLNLTKIDLKSLFNNFDVYTKSLVNTDKDTVINNLGETHSKLNEYIWVQRNKKFP
                     LDIIIVDNKIVGFICLSRETCTILIMDGYEEYTVLKEWEKTHKNEEIYSIRFGGNYMI
                     DMKDGIKLSTDVYLPDFVDSAKKAPTILMRTPYGKENDKEIYYKYVQRGYAVVIQDVR
                     GRNESEGKWEPMIHEREDGDSTINWIVSQEWSSGIVGMLGASYLGYVQWAAASSGNKH
                     LKALVSIVTSGSPFIDIPRKGGAFVSGMLAWAFMVSRNKVDRSKMVRDDWDDVLNIRP
                     IENIPVEALGYRIEFLEEWLKRVEKDEYWDLMDWHLQKDKINVPALVVSGWYDDNSMG
                     TTEALDVIKDYEKGKRKAILGPWMHNSNTLRDINGISLGNSSLRYDLDYNYLLWFDKY
                     LKGIENNIDTTAPVEYYSVGSNKWKTEENWPIINKIDKSMYLISDGNANTSLGNGRLV
                     FDNDLEEKYDSYIYNPKDPSVQLIDMSENEVGVPNNYKDLEKRSDMLCYTSDAFSEEF
                     TVTGDIKLEFFASSSAKDTDWVIKIMDVDLDGNSIKLADGILSARFRNSFYKSEFMEE
                     GEIYKFTIITSKISNTFKVGHKIRLDITSSAKNFIFQNSNTTEGYNSIEYIEAKNTIY
                     HGGKYPSKLILPIENK"
     gene            complement(26152..27312)
                     /locus_tag="KR250_001085"
     CDS             complement(26152..27312)
                     /locus_tag="KR250_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HBH0067049.1"
                     /translation="MNTLFSNPGSILAVMTSMIALGFYLQRYKAIKSLGPALTIIIMG
                     IILSNLKVVPVSTELYGTISTYAIPISMTIMLMSVDLKEMTKLSREPLIAIFVAVLTV
                     SIMAFLFGLVFAEKISEGWKVAGMFVGTYTGGSANLTAIGTGLNVSRQTLAAANAADY
                     VIGVPTLIFMFALPAILKNSKKFKKLWPYHVEESELEDCQNEEFMESKEWSIKDIAWM
                     LAIGFVVTEVSTILAGYFNSSFSSAARILLVTTISIIIAQLKPVKKLKGNLDLGLFVA
                     LFFLCTIGFSVDIKEFLGSTFTITLYCFSIIFASFVFHLGITRLLKIKYQYVILSIVG
                     AIADGPTSALVAASAKWNSLVSVAVVMGVIGGVLGNYAGISVAYAIKMSLGL"
     gene            complement(27331..28398)
                     /locus_tag="KR250_001086"
     CDS             complement(27331..28398)
                     /locus_tag="KR250_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HBH0067050.1"
                     /translation="MKITDIKFEKLRIKLKKPVVVSFGVIEYGESIILKIETDEGYCG
                     FGEAAPLAAVTGEVLDNVLSVLLMFKKELIGKDPLDIETIHTIMDGIIIGNTSAKAAI
                     DIALYDIKGKIMNAPLYKVLGGFDSKVQTDITISIDKPEKMAVEALERVKEGFRILKL
                     KAGINPEDDIEAVKLIREAVGESIRIRIDANQGWNVNSSINTIKKLEEFNVDAIEQAL
                     PHWDLDGTAYIRNKSNTKIMIDESLHSPIDAIKAIKKNAVDTFNIKLMKSSGIYPAIK
                     INNIAEASGVNCMLGCMLETRIGITAAANLIASKKNITEADLDSFMFCEELEGISGGF
                     VMDGDIMNLVNKPGLGIEVNL"
     gene            28750..29316
                     /locus_tag="KR250_001087"
     CDS             28750..29316
                     /locus_tag="KR250_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0067051.1"
                     /translation="MLGEKMRNIRKSKKKTLSDVSKLTDLSISYISQIERDAIEPSLS
                     SLRKIAEVLDTPLYMFMDDNNTDDLVIRKEDRVMMKFPKSEMFYEIVSPMPTTEFTPS
                     ILFVEFELKPESEDAKDYIYHASEEIMVVTSGVVDICMGEKVVRLNPGDSTFIKANVP
                     HKIINPSKDTIARGYGVISPPIWPIKSK"
     gene            complement(29511..30497)
                     /locus_tag="KR250_001088"
     CDS             complement(29511..30497)
                     /locus_tag="KR250_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase"
                     /protein_id="HBH0067052.1"
                     /translation="MGFKVELGGFQTLIQDRGRVGYGQYGVSGCGAMDEYAHRVGNIL
                     VGNSEDEASLEVLMLGPTITFDEYTQIAVTGGDLGAKINGKEIQNWRSYQINPGDVLS
                     FRGVKSGARAYVSIAGGIDVPLAMGSKSTYTRAKIGGFEGRALKKGDYINTFIQEKDF
                     TINKKLSSKYIPTYSSEIVLRIVKGPQFDAFSNGEVEKFLSNKYKVTNEIDRMGCRLD
                     GESIKHLNGADIISDGISYGAIQVPGHGKPIIMLSDRQTSGGYTKIGNVISVDLYKLA
                     QAKPNDVVKFELVDIYEAHRLLREQEDKIQDIYKSMKNIRVVKAKVLNDIAV"
     gene            complement(30527..31222)
                     /gene="pxpB"
                     /locus_tag="KR250_001089"
     CDS             complement(30527..31222)
                     /gene="pxpB"
                     /locus_tag="KR250_001089"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HBH0067053.1"
                     /translation="METRYLISGDKAVVAEFGNEISEDINKKVISFMRAIEISNLKGI
                     VTEMVPTYRSLMISYNPLEIDFDSLIENLKKIEDNLESIVLPKPKIHEIPVCYDEVFG
                     IDIKNVASYNNLTVDEVIKIHTSREYLIYMLGFTPGFPYLGGMDERIATPRLEVPRTK
                     IYGGSVGIAGSQTGVYPIDSPGGWQIIGRTPLKLYDENREEQILLRAGDFIKFVPITL
                     DEFIEIEKNNLSI"
     gene            complement(31242..32462)
                     /locus_tag="KR250_001090"
     CDS             complement(31242..32462)
                     /locus_tag="KR250_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HBH0067054.1"
                     /translation="MSTKNITDKTKNKKDVGALIGAAFIMATSAIGPGFLTQTAQFTQ
                     DFGPNFSFVILITTILFIGAQVNVWRVIGVSGLRGQDIANKIIPGLGYLVAFLVALGG
                     LAFNIGNVGGAALGMNVMFNMNMTLGTVLSGLIAIFVFMSKNSNSLVDKITKFLALGM
                     IIIVGYVAISNHPPVGEAVSRMVKPENPKGLIFPIITLLGGSVGGYITFAGGHRLIDG
                     GITGEENIKEITKSSLLGILVATMMRVLLFLAILAVVSKGLQLDPENPAASAFKFSAG
                     AIGYKFFGLVLWSAAITSVIGAAYTSVSFLKTLNPFIDKYEKYFIIAFIAISTLIMAF
                     IGKPATLLILAGALNGLILPITLGIMLIASKRKDIVGDYKHPTWLLIFGLIVVLISAY
                     TGITSLSSLGALFA"
     gene            complement(32491..33282)
                     /locus_tag="KR250_001091"
     CDS             complement(32491..33282)
                     /locus_tag="KR250_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamB/YcsF family protein"
                     /protein_id="HBH0067055.1"
                     /translation="MYKVDLNSDLGESFGTYKIGLDEEVLKYISSANIACGFHAGDPS
                     HMEKTVQLAKKNGVKIGAHPGFLDLIGFGRREMKITKQEAKDYTKYQLGALMAFASSN
                     GCNIQHVKPHGALYNMAAKDKELAMGICEAIYEVDKDIILLGLYNSEMINSAKEIGLR
                     FANEVFADRAYDNNGFLVPRNVEGAVIHDTKHAIDRVVRMVKEGTVETLTGEVIHIKA
                     DSICVHGDNPKAIEFVKEIRKRFELESIEVCPLENIEVCSSENIV"
     gene            33870..34499
                     /locus_tag="KR250_001092"
     CDS             33870..34499
                     /locus_tag="KR250_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBH0067056.1"
                     /translation="MDKNYTMPIYQKIALDIANKIYTGEIQEDSVLFGRSVLAGKYNV
                     SPETIRRSVKILEDIGVVKSIKGKGVIVLSPDKASSFIKKYRDITNISSYKSTLYNLI
                     DTKSNLENEILDTINKILDYSNRLEIINPLVPVQFTINSNCKYIGQTAAQTKFWQNTG
                     ATIVAIKRGEELIISPGPYIEFLEGDILLVVGDQHIYNSIPMFLYENEK"
     gene            complement(34613..36127)
                     /gene="glpK"
                     /locus_tag="KR250_001093"
     CDS             complement(34613..36127)
                     /gene="glpK"
                     /locus_tag="KR250_001093"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HBH0067057.1"
                     /translation="MEKKYVMALDQGTTSSRAILFNKKGEIVKIAQKEFNQIYPKAGW
                     VEHDPMEIWGSQSGVMREVIETAGIRPEEIASIGITNQRETTVVWSRYTGKPIYNAIV
                     WQCRRTSEICDELKNKGLEESIKEKTGLLIDAYFSATKVKWILDNVEGAREKAEQGEL
                     LFGTIDTWLIWNLTRGKVHVTDYSNASRTMMYNINDLEWDEDILRELDIPISMLPLVK
                     PSSYVYGHTDERMLSGAKIPIAGCAGDQQAALFGQNCVEEGTAKNTYGTGCFLLMNTG
                     SNIVKSKHGLLTTIAWGVDGKVTYALEGSIFIGGASVQWLRDELKIIESAKDSEMYAN
                     RVEDTNGVYVVPAFTGLGAPYWDMYARGSILGLTRGAKKEHIVRATLESIAYQTKDVL
                     EAMQNDSKLKLKSLKVDGGASNNNFLMQFQSDILNVDIDRPKIVETTALGAAYLAGLS
                     VGFYIGRNEITSKWSVEKEFNPNMSEEKRCKLYKGWKKAVSRALSWEKEDELDI"
     gene            36371..36913
                     /locus_tag="KR250_001094"
     CDS             36371..36913
                     /locus_tag="KR250_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBH0067058.1"
                     /translation="MPKILENVKEDILKVSRDMILEEDYSNISIRKIAQRCGISAGTV
                     YNYFNSKQEIIEYITKSEWDLLIRRIEYSNKNTEDYIKKLNIIFTEIRNFINKVHNIQ
                     YNDFLNTFETERFFEMKKHKDDFHEQLSNKVYEALEKESFFNNDKLVCNIIIKMFFSY
                     STYLHIEFEDLKPYIEKLIH"
     gene            36927..39419
                     /locus_tag="KR250_001095"
     CDS             36927..39419
                     /locus_tag="KR250_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBH0067059.1"
                     /translation="MNHNTSIKNKDSNPKIFLVLTLYLLGIFMGAIDTGIVSPARTII
                     QNSLGVNEKTGIWMITIYTLTYASVIPISGKLADKIGRKYVYLVSIFLFGSGSLICGL
                     SSLFSNFYILLIGRVIQAIGGGGIMPIATAEFGTTFPENKRGMALGLVGATYGIANIL
                     GSSIGSTILSIFGTQNWKWLFFVNLPICLIILIGGVFCIKNNKSESKEKIDKLGTLML
                     ICIIVSLLYGLMNIDFFNFKNSIQDISVYPYLLTFIVLIPIFIFIENRAKDPILSFEY
                     FLNPRILIVLILSLIVGIGMMGMVFVPQYAENALKINAGSGGYFVAILGLFAGIAAPL
                     SGRLIDKFGAKKILLLGFSVSMIGSLYLVLIALKTNTVFSVCVSLIFMGLGMGFTMGT
                     PLNYMMLSNTRLEESNSALATLSLIRSIGTSISPAIMIGFIAHAGLSVQDNIMDLVGK
                     PSTPKIVQLEELENMIDDLKSDPEMAKNLKNVSIPNMNDTSNINMDMTGGKLPKSLLN
                     KVQSADTTNITNITKEISTTMFDEKVPSIITKIQKNVQKGIDGTQSGINGIEDGENKL
                     NSGIKGVQTGIDNMVKARAGINQGIDGVKKGIAGVDKGIKGMEQGLEKQDEAIKELTT
                     AYNKIPTTEKTPTDNENNSNEQNQSNTSNSESDDTKNDSTENKNNNSSENKPNVNEQK
                     ESLNAQIQQLKKTRDDLNTKLQKNKTQKNELSKKLRSMESQKKELQSKLNNSISQKKD
                     MEKSLDTMQQQKKSLQAVLAKTQEVKTEIPKAFDKSKLDYINSIENNRTKIENTLQST
                     LNSGFKQMYITVFCVNLLAFIILLFYKENKTR"
     gene            39840..40238
                     /locus_tag="KR250_001096"
     CDS             39840..40238
                     /locus_tag="KR250_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH0067060.1"
                     /translation="MDTLGKRIAYLRNSKKLTQRKLMDILKFENLGKYETGDRKPNCD
                     ILMSIADYFNVTTDWLLYGKEKVNVNSSVKEDKEDYLHVTNDEMTILNLYRQLNERDK
                     IKIEGILELKISEYKDLKKHSSNNNEDKMV"
     gene            complement(40455..40664)
                     /locus_tag="KR250_001097"
     CDS             complement(40455..40664)
                     /locus_tag="KR250_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0067061.1"
                     /translation="MCKLDSDGVIECCRAIDDFITALSNIKSLNMERLNTLTKYSSTC
                     SILLKEGNYEGCTIVYRKMLEELKT"
     gene            complement(40696..40905)
                     /locus_tag="KR250_001098"
     CDS             complement(40696..40905)
                     /locus_tag="KR250_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0067062.1"
                     /translation="MDARKKWIPFLGVQVKQRLIELNMTQRELAKKIGVNENYLSAIL
                     NGRRTGKKYKSSIYQLLNIEYSEDD"
     gene            41204..41623
                     /locus_tag="KR250_001099"
     CDS             41204..41623
                     /locus_tag="KR250_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0067063.1"
                     /translation="MDTLGERIVYLRKAKNLKQYELEEMLGCDNLSKFERNIRKPNYE
                     ILKSIAEIFNVSVDWLLNGDNLSHKSDLICDSSSNYPLNSINSNEIKLLNNFRKLNDY
                     DKAKIEGMIELKLHEYEKEKDLGKIEYNKNKDEKIDK"
     gene            41851..42306
                     /locus_tag="KR250_001100"
     CDS             41851..42306
                     /locus_tag="KR250_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0067064.1"
                     /translation="MGLRDKFAQSFARSKTMSGPEKKANEIMGKLLLKKAILPIVLMF
                     VIIIAGAMLKINSWVTLGINLVIAVGAFFYIRNSSKKYQNFKPYVGNLISLEKKGKKE
                     YVAIIKQGKLPVKLQIAYGGEDLEHVKKNQMVQISYNPDAKIAILVNRQ"
     gene            complement(42513..42773)
                     /locus_tag="KR250_001101"
     CDS             complement(42513..42773)
                     /locus_tag="KR250_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0067065.1"
                     /translation="MDKLITELSSLGAIGILCALLFKNTMQEKKEDRDMYKKTVENFI
                     ELSTQQQEINKNILVQMGIMKTDVEEIKEDVTDIKGMLQNGV"
     gene            complement(43036..43389)
                     /locus_tag="KR250_001102"
     CDS             complement(43036..43389)
                     /locus_tag="KR250_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0067066.1"
                     /translation="MFKFDKNKIEQIKQGRKVEMQYKDISDISIGQVKQDDDITNNFI
                     ANVEIYEMLLNQSSVNEASNISTFSVRKSGGESGMVEVYVALILRGKKTIEEVPAVIR
                     EQVRIRCKELEIPVE"
     gene            complement(43406..48727)
                     /locus_tag="KR250_001103"
     CDS             complement(43406..48727)
                     /locus_tag="KR250_001103"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012632.1,HMM:NF024930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0067067.1"
                     /translation="MKRTKLLQRGNFFGDKNMVVDEFDEGYDNYDFINFFTGCCNYTF
                     GLKNNNILYGCGDNSNFQLGLGEDNTTRKLFTKIPNISTNIKKVACGESHAVILTSDG
                     ELLVAGINTDGQMGLGLEKVGKTVSTFEKVPEIKGVKDIACGLQSTYLLYNDGTLYVA
                     GNNLYGQLGLGTNGASANVNTFTKVDVDNVKAVFSYNKSAFIIKNDNKCYSTGFNNQG
                     QLGLGDKNNRDLFSLVSINDVKTIACGSEHTVLMTYNNDIYGCGKEKCFGNALQSSLF
                     TKIEEVNIKTIACGHGNTMLIDNKGTLKVAGNNDIYQLGIANYSENIDNSFIDLKNIV
                     AKNIFIGLSHSILIDSNNDSYCTGDNTYGQLGSFFDDMHIVEFKKMDSEKYSYSNYIN
                     LIKSEDKLTLLKEEMEIKDIELPLDIHSVRDVVFSPYCTLVILGNGDVYGLGNNRYKG
                     MGSDLPSQLNELTKLSISNVKSIVASKNISGGIFYIKNDDTCYYSGPNSNSIAGVLPS
                     NSDVFKKISIDNVKKVVINTDLSNWFSLIVTNNKQIYTSGKSSSYVNGLSNALISQYT
                     EISLSNVTDAYSSYNATFIVVDEKKVYATGINTNYLLGFSTSDGSNVNLGLLSDWYYI
                     NISGSSYSRVSCTNNITKINNIIIYEYVTVFCTNIGSFLTGYHGTSWTKPTDSSYRVQ
                     YQGISYAGYLDSYIYNYYPTRCTQSSSSTTFAYLYNGESSSNLKNVNPDNLLISGGSS
                     YIHQYGRNYLNNQSSNNIAASNINSGPITSDKAIFLYKALLYLSSNTLYGFGNISESA
                     KELDVSDTQDGYNATNYKKVMKNIKNIFIPPYDLSRDKTRFAILTDKSLFICGYNSKG
                     THGISVNSSLNLNNKINYNKKNSSSEISSNIQEIYSHSKSTYLLTNNNMLYSVGLNDV
                     GQLGVGDEINRKVFTKINIDNIKSINVNRFTDNSKHAFAIKNDNTCYAVGLNNSGQLG
                     IGDNVNRNIFTKINVENVKYVAVYGNTSLLLTNDGLLYGAGNNGKGQLGLGDTTSRNI
                     FTRIPINGVRDVYLCNDVSIIVKNDNTCYVCGLVNGYFGFTEGSISTFTKINIENVKS
                     VVTAGSEATFFITNDNMIYTTGKKERVFFSTETNDIKGIRVINNIINAKKIVVNGYTS
                     AILTNDNKLFVGGLSGYGSIANNNNTNSVEDVKDVFVTANNTLYIDNNNNLISSGRDT
                     YGISDESYRDMSVPYYKVSIKKDVDTVFSSYNTIFIKDIYGKFYSSTGDNRYNHLGIH
                     HRYDNDKNEALEGSLHSYFKTDNTSDKIVFNKKNEKLVMFNDKYIKTNNKYINYKNIF
                     KDNFKYTSIILPFEVSDIDISKTHSLAVAKDGKLYGIGSNSYKEINQTLEDIELLTLT
                     EVNISDVKKVACGDNYSYIIKTDNTLWSYGKNTEYQLGVGHNNDVRELQKVTGLPSVK
                     DISIYNSMTLVLTNEGELYAQGYNTNGLFGLGESEKDKIIRTFTKVLTNVKEIKSHND
                     DHILVIKNDNSLWITGKNKSMYKISISITDLYEFTKIPIPEHLNDILDIELSDDTIYM
                     ITKVDTSKASIEIVEKSISQVRVVVQDPNNVIEKLEMFINDELISTKTNLEINSIIFE
                     IPQNKIVLGENKILIKASSPTGDLYSSMFIFKSETGLKVKKDSILMINNKVYSIINIT
                     ENNTDLIVTLNEGLKDDMMENNPIYQLINKTKVQVKINKSDLFKDMKLVEIKKSDSSY
                     QEIYELEEANIKSAQPKIIVEKGDKWTAIKRPSMIFRYDAENNEPQA"
     gene            complement(48744..49727)
                     /locus_tag="KR250_001104"
     CDS             complement(48744..49727)
                     /locus_tag="KR250_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBH0067068.1"
                     /translation="MEEKFYIILTKIGREKIANATALGELVGLTKFQVGDSNGEYYEP
                     TEEQTALKNVVWEGNINSLRIDEKNPNWIVIETILPGTVGGFMIREAAVLDNENNIIA
                     IGKYPETYKPRAEDGSIKDLVVKMILQLSNTSNVTLEVDPTLVFVTQKDIQDLDDKFD
                     KNIKEIKVKIGEELLSTEAKNLSGAINEVVEKIKNISIDDVIGGQIQTELSVLKNSYN
                     KLSEKVLDILIYLELESEIDVDEAGYWYDTLTNAKNIIAIEGLKLDLNRKCITGELGS
                     VTFKNVVLPFNANRVRYIHEMDNNFVETKSNRAYSIGQTDITLNKYSYEIR"
     gene            complement(49728..50426)
                     /locus_tag="KR250_001105"
     CDS             complement(49728..50426)
                     /locus_tag="KR250_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2313 domain-containing protein"
                     /protein_id="HBH0067069.1"
                     /translation="MKLIDKLPSFDRNYIVEEIQGAYDTELNILKEDIDDTFNQLFVD
                     TATWGLDMWEDILCIEKKELDFDTRRSNIKAKMRSRGTSTIEVIKSICEAYTKSETDI
                     KVYSDEFTFVLSFIANNCDYKTLLDCSDMIERVKPAHLLHYLEPIILDKSMVYCGGGM
                     VCSEEVKVHPYFEPIIKCSAVVNCGAGMISREEIKVYPLSIKCIENNCKINIAIANDT
                     GVENVVVYPKSEVV"
     gene            complement(50423..51475)
                     /locus_tag="KR250_001106"
     CDS             complement(50423..51475)
                     /locus_tag="KR250_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HBH0067070.1"
                     /translation="MYSDQTYEVIKNRTLENINLDIYKGEGSFLNNMVSGNNLELSKI
                     YLELSKMHKMAFIQDTYNQFLDKRVNEFGVYRKLGTESNGEVEFIGEKGTVINNGTII
                     SYRDLLFVVIKDVTIGSEEGDNSPVQALEVGKKYNLPTNCEFKLVDNISGVTKITNTR
                     SFEGGTDIETDEELKERFYKIQRNQATSGNKAHYEEWALEVDGVYNVKVYPRWDGPGT
                     VKVLIFGKNNQAVDTETIERCQQHIDEEKPIGPTITVVTPLPIEISISAVMKLEDGYT
                     LDNVKESFLESINTYFRDIRGEIIYTKVMGILINTTGVHDLSNLLINGSTDNITINED
                     KIPSVTTVNFSEVENQ"
     gene            complement(51468..51896)
                     /locus_tag="KR250_001107"
     CDS             complement(51468..51896)
                     /locus_tag="KR250_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HBH0067071.1"
                     /translation="MSTIFPFIGVPEDYILPKTEELPIFREVAWDFEKDEPILEKGDF
                     KIIEKKEALKVWIYKCIKTNRYEHEIYSLEYGTELSELIGQKYTKGLTESEASRFIKE
                     ALLINPYILEVNVKSANFNRDILSANVKVSTIYGEVEINV"
     gene            complement(51896..52222)
                     /locus_tag="KR250_001108"
     CDS             complement(51896..52222)
                     /locus_tag="KR250_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2577 family protein"
                     /protein_id="HBH0067072.1"
                     /translation="MANPINEFIGIIREEGKYHNQPSFFIGKIKSKLPDLKIETNNII
                     LEKEDILIDSWMIDRQLETFDTETNQEHQHEVKNPFIDNFESGDMVIMFRIGEKFAVV
                     SKLVSL"
     gene            complement(52238..53767)
                     /locus_tag="KR250_001109"
     CDS             complement(52238..53767)
                     /locus_tag="KR250_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBH0067073.1"
                     /translation="MIINRSKDSSSNEISFVSKDMGFLLTQSEVSYNFKDKLVEDIAK
                     QVFAENRLSVGIIAKTNVKYTKMFIGVNGYDTIMSAYTEASKKTKKKYMIEANLDKFN
                     VIEKGTVTLSVMFEEGFNIINTTFSESMENVKNKVIVVDQYGSKISEKIDNEIFKEVN
                     VIMQKVIQQQENQDVDIDSEFNGIEKSCSLKGYGDVSCITGRGVKVKDSYTKLVGLFY
                     IDTDKHTWQNGEYQIELELNFQNLMDEKSAGQDEPKEESNLGGEDYAGGKEFTAEFTA
                     YCPRKEEGGDTDCRKKKLDPSKKTCAAPMVGKYEQTYYTKEFLNKHPLLNYGDEIQVI
                     TGVSGRDGVYKVNDVGPAITIEKNGTYHIDILFGNVEEASKFGRRKGKIIIGGYSGNV
                     SDKAKIVISEAKKHLGKPYKWGGNGPSSFDCSGLMVYCFKKVNVSLPRTSNQQSKKGK
                     KVEQKNLQAGDLVFFHNPVSHVGLYIGNGEFLHAPQKGDVVKISKLSSRRDFNTARRV
                     L"
     gene            complement(54049..54471)
                     /locus_tag="KR250_001110"
     CDS             complement(54049..54471)
                     /locus_tag="KR250_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0067074.1"
                     /translation="MEMWLRQAEDRFRFPVFPSSFSINGKAAVNSSSILKIGEIATFG
                     GVALKSISISSFFPNKDYTFCDYTGFPSPYDCVNKIEKWMKEGFILRFTITETNINME
                     VIIEGFSYEERDGTRDVYFTLDLKEYKRIKIPKVTPKQ"
     gene            complement(54471..56924)
                     /locus_tag="KR250_001111"
     CDS             complement(54471..56924)
                     /locus_tag="KR250_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0067075.1"
                     /translation="MGNVREEGINMYLTDNYTPKMNQIISVTDNFRRATVAVSLSTNV
                     MASSIKNSIGSASNRVNSLNSSLRKVQTTASSVSSTMTKLSSSINAVSGVIGSLNGSI
                     MRLAITIAMIIDYFNKLIQKKNEFNSNIMIILIFKAKSDEVEKTKNKLLGNLKKIGGK
                     IWNIVIKAKDMTKRVISSILGKLKQVEKRPYQGSINLKDMVSSAMGRILPKLMLFKNT
                     FWSGVIAIKDMASGIISKVFPKLRLFAGKVWSGAIAVKDMASGILGSIKGKISDLTNG
                     ATIGVAVKKGVDLLGQEQNQKVVLESVMKRNTGKVNQIDVDDYYGSLVRMANDTPFDP
                     EDVVAMGTKAKMISNITGGKKEKDITQAMVDVRALNMNTSSEQDVSAAFLSAAKGNME
                     SLNTLVGENYKTFDEALEGISVKQMGLAKEMSNTIPGIISGAQTSINNGLKSIVKPFD
                     DILGQGLKKIKTFIESGLGNLAGLSEKMAGKIGNVMNGKIIIGNKYDQMQSRSVKNGK
                     EFSDSTQYRISNEAEKRKMMVENKQERFENHAATMIGNAPKAIVNAGSTLLQNIDFTA
                     LIDSLLPVVNLVNNLLDSINNKSPIAQGLISIFGTIVTTAFQLIGPVVEAVSPIITRI
                     FTFLGEYAPQINNFIETLGVIWKTVWETLGPLLETGWKIIEPILGAFFNILDKVCKIV
                     KDICKWWQTMINKIKNGSITGTVLNLVEKSKKNYKDNPYAGTKAGDSGKAYSGKKGNN
                     AFGLNYVPYNDYQTRLHEGEMVLTKQEANQYRSRKNGGNINIAKLADTIVIREEADIE
                     KITSKLVASIQLAQLGGVL"
     gene            complement(56924..57091)
                     /locus_tag="KR250_001112"
     CDS             complement(56924..57091)
                     /locus_tag="KR250_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0067076.1"
                     /translation="MYYYWKKKGIRPSLFYAMDKGELKLIEAFFALEIEEEVEKMKHG
                     YGVCPLTGGGM"
     gene            complement(57118..57564)
                     /locus_tag="KR250_001113"
     CDS             complement(57118..57564)
                     /locus_tag="KR250_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBH0067077.1"
                     /translation="MSENGLSKNINIVDLLLNSDTENLERPSTIVELKRLSTIFGQEF
                     KVMCRALTISKDEEIQNTCLKIDENMKTDIDLPEMQMLTIIEGVCDLDGKLLFKNKEL
                     MDKFKAPTPKELARKLLLPGEITNLYRILQDVMGYGKNAVIEEVKN"
     gene            complement(57678..58106)
                     /locus_tag="KR250_001114"
     CDS             complement(57678..58106)
                     /locus_tag="KR250_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HBH0067078.1"
                     /translation="MANMEARNVMSGTWGELWLDGNKVAEVKKFQAKMEFTKEDIIIA
                     GQMGTDTKYMGYKGKGSITLYHVSSRMHKLIGEKIKRGSEPRFVAISKLNDPDSYGAE
                     RIAVKNIAFDDLTLADWEVGVKGEIEAPFTFTEYDFLDII"
     gene            complement(58120..59184)
                     /locus_tag="KR250_001115"
     CDS             complement(58120..59184)
                     /locus_tag="KR250_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HBH0067079.1"
                     /translation="MAIGLPSINISFKELATTVKERSARGIIAMVLKDAKALGLNEIH
                     EKEDIPVDLSAENKEYINLALMGNVNTPNKLLVYVIEGEADIQTALDFLETKEFNYLC
                     MPKAVEADKTAIKNWIIKLRDIDKVKVKAVLGKVVGNHEGIINFTTEDVLVGEKKYSV
                     DEFTSRVAGLIAGTPLSQSVTYTKLSDVVDIPKMTKVDAESRVNKGELILIKEAGAIR
                     IARGVNSLTELTAEKGEMFQKIKIVDTLDIIHSDIRKVIIDDYIGKVTNSYDNKCLLI
                     VAIKSYLEELEKSALIESDSTVEIDFEAQKSYLKSKGVDLSYMTLQEIKEANTGSKVF
                     LKAKIKVLDAMEDIDLSIEI"
     gene            complement(59189..59632)
                     /locus_tag="KR250_001116"
     CDS             complement(59189..59632)
                     /locus_tag="KR250_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0067080.1"
                     /translation="MLKYKEILETIIEILKKNFTESIFIDDESVQGSEGSCFFVSILS
                     VICTPVMLNTNNKDIVISIKYLPKPQSKSIRMYEISDELNKLFNRNIKVTDRKLNITK
                     LEQSIKKEESIYVLNFTFTLNYLDSVYEEDVVYENMKEINLNLGE"
     gene            complement(59881..60321)
                     /locus_tag="KR250_001117"
     CDS             complement(59881..60321)
                     /locus_tag="KR250_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0067081.1"
                     /translation="MENKKDILFKETDKRLHNYKYLDIKIKNINLDIKRCENEYSGCG
                     AMVYTEKTSNTYNISSSVENEVLKREERLRKLKMEKEDIEIEKEKIENALTCLNDIEM
                     EFFNLFYNSKTKNNMTYISMKLHLDRTSCYNLKKKMIFKLSEIL"
     gene            complement(60426..60623)
                     /locus_tag="KR250_001118"
     CDS             complement(60426..60623)
                     /locus_tag="KR250_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0067082.1"
                     /translation="MFKNNLKYYRKCKGMTQIQLARKAGITNDYISQIERGIKNPGLL
                     MAKKISSILEQNIEEVFFIQL"
     gene            60792..61112
                     /locus_tag="KR250_001119"
     CDS             60792..61112
                     /locus_tag="KR250_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HBH0067083.1"
                     /translation="MFAKRLRELRKEFGLTQRELGEKVGVSQRVLGYYETENRFPDEH
                     ILNKLADVFNVSVDYLLGRTLVKENIDTVAAHRKNPHEELPEEAQEQLNDYIEFLLNK
                     YKKK"
     gene            61194..61700
                     /locus_tag="KR250_001120"
     CDS             61194..61700
                     /locus_tag="KR250_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0067084.1"
                     /translation="MNNLDKLFELASQEEIIIHYTTYIAGDLEGLYINKHGIKIISLL
                     SNLKQNSKKLTSILAEELGHHFTSLGYYVSSYNDYYTKIIIDKCENKALKWACEFLIT
                     EEDIINIINSGITCVYEMADILNVDITFFQKRLEFLSLKKQSLQLGNNKYLILTNLPY
                     FYIFDPIS"
BASE COUNT        21029 a        10145 c         7261 g        23566 t
ORIGIN      
        1 ggccatttac tgttattatg aatatggttg aaattattta aataaatact gttgacttag
       61 tttcatatat ttaccattaa acttttatca taaatatcat atcataataa tatatttaat
      121 aagtctaata aactatacga ggtgtaaata tgttaatgaa tacccatttt attatggcaa
      181 aatcaatact tgataatata gatgaaaata aaacattctt tataagcgaa aaaaatttta
      241 tatatggcaa tataaagcct gatgcttttt ctaaatataa attaaaaaaa cattatttag
      301 atgagtcatt taatatgatt gtttctaaaa taaactatct atgcagtctt aatatagata
      361 gtctttctaa atacttttca gtaagtagac ttagtcagga actaggagtc atatgtcatt
      421 ttttatgtga ttttttctgt gtagctcaca gtgaacgttg ggagtttaag catagcttaa
      481 ataaacatgt tacttatgaa agagaacttg gagcatttgc aaaagatgtt gatttatcta
      541 aaattaaatg tggtactata aatagtagtt ttgagaagtt ttttactaaa ctttatagcg
      601 attacaaaaa gaaaactgat tatatgaatg atttaaaatt ttcagcttat acttgtaata
      661 gtgtcataaa ttatatactt gactctattt tatctaatac aataaaatca tatcatatag
      721 caaattgcgg ttaacttaag aatttttaaa cttatataaa cttataattg aatataatta
      781 aaatgactgc tctatagaat ataattttat acttctctag gacagtcatt ttttatacta
      841 actatttatc tccattataa catctatggc actttttaca ctatctacta cataatttgc
      901 actttgcttt acatcttccc ttgccttaga cattgcaaaa ctatatttaa atccttctaa
      961 catgcaaata tcatttggag aatccccaat agtagcaact tcatctggac ttacatttaa
     1021 cgtttttatt acatgctcta tacttcctct ttttgacaca cttttaggaa ctatatttaa
     1081 agtatatgtt ccagaaaaat atatttctaa gacttcacta aactcacttt ttagataaag
     1141 ttctattctg gataactctt ccaaattttc agaacatacg cctatatttc ctataagatt
     1201 atcaaaacta cttctttttt caaagtctgg gtcaactata aagtcactag aataatgtct
     1261 aaaagcagtg gtatcactac ccacaattac atcaccctta tatttaaaat aaagttgtgc
     1321 atcacttgac tcaaatctat caatgacttt ttttactaaa ttatcatcta tcgttctttt
     1381 aaaaattaca ttataatctt tatcatatat aaatgaacca ttttcacata catagtatcc
     1441 attcatatct aaactatctt tgataatatt atgtgcttgt ttaaaaattc tacctgtagc
     1501 aacattaaac tgtactccct ttttttctat ttcttcaatt gctactatat cttcttttgt
     1561 tatggttcca ttttcatata gagttccatc taaatcacaa aatatatgtt ttatcataaa
     1621 ttaaaagcct ccaaaaacaa ttttatattt tactaatact ttaatcaaaa taacttaaag
     1681 cttttctaat tgtattctca actcattaag caaatctcta gtagctctta atcttatctt
     1741 tcttctatcc ccattaaata tatttttctt tactattgtt tttccattta tatatattgc
     1801 agtataaacc agtcccacag gcttctcttt ggttcctcca ccaggtccag ctactccagt
     1861 tgtagatata cctatattag ctttaagtcc tttagcaacg ccctcagcca tttctatagc
     1921 tgtttgctca cttactgctc caaactcttc caaagtttct ctttttactc ctaatctttt
     1981 cattttagaa tcattagaat atgttacaca accttcaaga aatacagagg atatacctgg
     2041 atagtttata agagatgaag aaaccaaacc tccagtacaa gattcagcca ctgctattgt
     2101 tagatttttt tctacaagta gcttagaaac agcatcttct aaagcagtct ccccttcaga
     2161 tatggaaata tctccttcag tatatacata tttaccaact ctacttttta ttttttcttc
     2221 tactggtttt attaatttaa aagcttctct ttcattttca gcctttgcag ttattcttat
     2281 tgtaacttct aattcttttg catataaagc aactgttgga ttagtctgct cattaattat
     2341 atcgagaatt tctaattcta gatttgactc acctattcca tataatctta aagtcttaga
     2401 tactagcatt tcatttgtga attgttgtaa gtaaggtttg acactctcat taaacatagc
     2461 tttcatttct cttggaggtc caggcaatac tattataaat ttaccatctt tttttaaaat
     2521 tgcccctgga gctgtaccat tattattttt tagtactata gcatcttttg gaaagtacac
     2581 ttgtttcata ttattttcgg ccattttttt ccctgtttta acaaagaaag attccaaaac
     2641 ctctagtgaa ggtttatgta gttctaattt ctgaccaaag tattctgcag cagtttcttt
     2701 tgtcatatca tcaccagttg gcccaagacc acctgtagta attacaaaat cacttctctt
     2761 taagctttca tcaaaacatt ctaaaagtct ttgtttgtta tctccaacag tactctgatg
     2821 gtatacttct attcccaatg atgcaagttc tttagctaaa aattgagaat ttgtatttac
     2881 tatatctccc aataatattt ctgttcctac acttattatt tcagctttca aaatttatca
     2941 ctccttgatt tatagccatt aagactagtc ttaagttcat aacaataatt ttagcaaaaa
     3001 taataacata aataaagtta aaaaatcatt taaacatatc tttaaactag attattattc
     3061 taaaaccaca cataatataa acttcgtata tagtggtact ttcatcctca actgttaatt
     3121 catataatta agtatgtaaa taaaaagttt actttaaaat aataaaaatt aaaataggag
     3181 tgtataatat gaaaatagca gttataatgg gtggaatatc atcagagaga gaagtttctt
     3241 taaattcagg aaaagagata tataataacc ttgataaaaa taaatatgaa gttgtaaaag
     3301 taataataga tgataaaaaa gatatattta caaagatacc agaagatata gattttgcaa
     3361 tacttgcttt acatggaaaa tttggtgaag atggatgtat acaatcaata cttgagacta
     3421 tggatatacc atactctgga tgtggacctc tttgcagtgg gatgtgtatg gataaaaata
     3481 taactaaaaa aatgcttaga gattcaaacc tacctactgc tccatgggtt ttagtaaaat
     3541 cagttgatga aatagactat gatgaaatag ataatatagg ttatccagta tttataaaac
     3601 caaactcagg aggttcaagt gtagccacat tctttattca ttctaaggat gaagttgaag
     3661 aagcagttag aaaaggtctg gaagttgatg aatttgtaat gattgaaaaa tatataccag
     3721 gtggagaata tacttcattc atattaaatg gagaagtgtt ccctacaata tctataaaat
     3781 ctgattcagg attctttgat tatgaagcta agtactcagt tgaaaaaggt gcaaaggaag
     3841 aagttgttta cttagatgaa gaacttcaaa aaagagtaaa cgaaatttct gagacttgtt
     3901 ggaaaatttt taattgcaaa gcatatgtaa gagttgatat gattataagt gaaggtattc
     3961 catatgttct tgaactaaat actcttcctg gaatgactca aactagttta attcctagaa
     4021 gtgctgctgc tagaggtatt aagtacagtg aactattaga taaacttata gaatactctt
     4081 taaattagat gaaataattt gaggtggtaa tttgatattt tcaaatctaa aactaaatga
     4141 taatgaacca atttatatac aacttaaaaa ttatataagt gatatgatat caaaaggact
     4201 aattccagac aatagtaagc tcccttctac aagggagctt agtcagcttt tacaagttag
     4261 ccgaaattct gtagttttaa cttatgaaga attaaaatca gaaggattaa tctactctat
     4321 ttcaggaaaa ggtacatttg ttaaatcaaa gaataagtct tcaaatacta catggtcatt
     4381 aaactgggat tgtttagaga atacatactc taaaaaagcc aatgaattgg acattataaa
     4441 aagtgaaatt ccttggagct ctgacctgat ttcctttaaa agtatttctc cagatggaga
     4501 tttattcgac atagaagaat taaaaaaatc ctttttaaat agaatttcac tagaaggaca
     4561 taaattactc aactatggat atgcacaagg ttataagccc cttattgact atttactaga
     4621 atatatgaca aataagggtg ctgatataag taataaagat atactaataa caaatggatt
     4681 cacagaaggc tttgatatta ttatgtcatc ttttacccaa gaaaaagact acataatatg
     4741 tgaaaatcct actcataata ctgctataaa aattatgaaa tctcataacc tcaatatctt
     4801 aggtgtagat ataaatgaag atggtttgga cttcaatatg ctagaagaca atttagttaa
     4861 gtataaaaat aaaataaagt ttgcatacat tacaccctct tatcacaatc ctacaggaat
     4921 tgtaatgact cctgaaaata ggtacaaatt ctataatctt atgaaaaaat acaatatagc
     4981 aattatagaa gatggattta atgaagaatt actctacaat agttctcata tatttcctat
     5041 ctgttcccta gataatatga ataatggggt cgtatatatt ggtagttttt caaaaatttt
     5101 atttcctgga atgagaattg gatggatatt tgcagataaa aaagtaatca ataagctaga
     5161 aagtgtaaaa aggtgcagaa acatacatgt atcctttttg gaccaaggaa ttttatttga
     5221 ttatttaagt aatggaggtt ttgaaaaata cattaaaaaa atacgtaaat tctatgggga
     5281 caaatttaac tttgcatata agtgtataaa aaaatatata aaaacagaat atatcttggg
     5341 tgatggtggt ttgcatatat ttataaaact aaaaaatata gatactagag ttttattaga
     5401 aaaatgttat gaaaaaaatg ttatatttat gcctggagat ttatttttta cagataactc
     5461 tggttttgat actttaagac tcggattttc taggttgtct tttgaagaca ttgaaattgg
     5521 aataaaaata attggagaaa ctataaatga aatgttctag gttaactaaa tattgttagc
     5581 ctagtttatt tttatgaggt ataatatata tagtttatat gaatctaata ctgggggata
     5641 atatgagtaa aaagtcaaaa cgaagaaaaa ttaataaatt aaaattaacg attgtaataa
     5701 ctgtattatt tcttgcttta ttatctatat atgagatact gttctctaat cccaacaaaa
     5761 aaggtaatat aaagagtcaa gaaactatgc aaaataaaaa taatacaaca gatgattcta
     5821 aaaataatca aagctctacc aaaaagcagg aagataataa tatcaagcct actttttcat
     5881 attcttcaat accagataat ataaaaaata aaatgattgg taaatctatg cctactgatg
     5941 aacctataag ctttgatagt ctatcttatt taaaacttac atattatgga tttgatgaaa
     6001 aaactcatca aggagaaatg atagtaaaca gtgaactagc tccagaagta gtggatattt
     6061 ttaaggaatt atacgaaaaa aaatatccaa ttgaaaaaat taagttaata gatgactatg
     6121 atgctgtaga tgaaaaatct atgtctgata ataatacctc atcattttgt tatagaacta
     6181 tagctggtac aaatgtagtt tcaaatcatg gtaaaggacg tgctatagat ataaacccat
     6241 tacagaatcc tcaagtttct ggaaatgatg ttacacctaa agtaagtaca gtttatgctg
     6301 ataggtctag cacaaaattt ggaatgataa aaaaaggtga tgattgttat aatgcttttg
     6361 taagcagagg ttggagttgg ggtggctatt ggaaaaaccc agattatcaa cattttgaaa
     6421 aataatagat tagaaataaa ggtgaattat atgaatttaa tgacattgga aaatataagt
     6481 aaaagctatt ctgaaaaaaa attactagaa aatatatctc ttggaataaa tgaaggggaa
     6541 aaaataggac ttattggtgt aaatggtact ggtaaatcta ccttactcaa aattattgca
     6601 ggagttgaag aaagtgagac tggtaatata acaaaaacta atggtattag gatagaatat
     6661 ctacctcaaa atccaactta caatgaaaat tcaactgtac ttgaacaagt atttaatgga
     6721 acttctgaag aattaaagct acttggtgaa tatcaagaaa tcctagaaaa acttaattca
     6781 aactttacag aggaattaaa taaaaaatta ttatcagtac acgaaaaaat agatgcctta
     6841 aatctttggg atttagaaag tgaagctaag tcagtactta caaagcttgg tataaatgac
     6901 tttaatcaaa aaatagcaga actttctggt gggcaaagaa agagagtctc tctggcatca
     6961 gcccttatta caccttgtga gtttttgata ctagatgagc ctacaaacca tctagacaat
     7021 gatactatag attggcttga ggaatatctt aattccagaa aaggctcatt gcttatgata
     7081 actcatgata gatacttttt agaccgtgtt acaaatagaa ttattgaact ggacaaaggt
     7141 agattattca gttatgatgg gaattattca atatttttgg aaaagaaaat ggaaagactt
     7201 tcgctagaag caagtattga agataaaaga caaaatttaa taagaacaga gcttgcgtgg
     7261 gtaagacgag gagctcaggc tcgtagcact aaacaaaaag ctcgtttaca aagatttgat
     7321 gaacttataa atagagattc ttttaaacct gatgaaaatc ttgatatatc tgttgcatca
     7381 agtagacttg gaaataaaat aatagaaata cacaatatat ctaaatcatt tgaagaaaat
     7441 actgttatag ataatttaga atacacttta actcgtacag atagaattgg tataataggc
     7501 aaaaatggta tgggtaaatc tacccttata aatatactta atggagaaat agagcctgat
     7561 agtggatata tttcaattgg agaaacggtt aaaataggtt gtttttctca agacgactct
     7621 catatgaata ttgatatgag ggctatagac tatgtaaaag aagctagtga ctatatagaa
     7681 acttcagatg gtactaggat tacagcttct caaatgtgtg aaagattcct tttcaatggt
     7741 actatgcaat atactttgat aggtaaatta tctggtggtg aaagaagaag actacatctt
     7801 cttaaaatcc ttatgacagc tcctaatgtg cttcttttag atgaacctac aaatgattta
     7861 gatattgaaa cgcttaaaat attagaagaa tacctagacg aatttaaagg tgtagtagtt
     7921 acagtatcac atgatagata tttcttagat agaatttgta ataaaatatt tgcatatgaa
     7981 ggtaatggaa aaattcatat ctatacaggt aattacagtg attatcttat ttatagagag
     8041 attcaaggaa ttgaatttga agaatctaaa aaggaatcag ttaaaaaaga tgactctact
     8101 accctaaaaa aagaaaaacc taaaaatgat aaaaagctta agttctcata taatgaacaa
     8161 agggagtttg aaaatattga ctctgatata gaaaaactcg aatctaaaat tgcaacactt
     8221 gatgaaagta ctgcaaaatt ttcaactgat tttactaaac ttcaagaaat tttagatgag
     8281 aaatctaaac tagaaaaaga acttgaatac aaatatgaaa gatgggaata tttaaataat
     8341 ctagctgatg aaatagctaa taataagtaa tattaaaata atcttaagtt taataagttt
     8401 tgctttaaat ctgtaattta taaatatttt aaaggaagtg tctcaaaatg aactttttag
     8461 ttttatgaga cactcttttt tatgagaaat caaatatatt ttcatcattt caacaataca
     8521 aaagaggctt atctctattt tgagacagcc tctttcataa ttaccttcta atttatatgc
     8581 tctaatatat ctataataat tcttattata tctatatatc cagtctttca atataatgac
     8641 aattaacttc aatatgattt aaaaatacta acaacctttc ataaagtgga tgagattctt
     8701 ctccatattt agcttctagg ttcttgccta tttctctaac agtcctttga ccatcaattt
     8761 gtaaaaaaac aaaactggaa tattcatcta gttctatttt tttgtattta ggtattctag
     8821 cttttaactt tctaaaaaac ttttgaattt tatggtcctg tttttcaagt atagtaacaa
     8881 ctctattttc agctacttca tattcaagtc catcagtaat tttaaataca atatctaaaa
     8941 catcattact cttgctcatt attttttact ttagataaaa caataggtaa tgctgtaagc
     9001 acaactaaaa ctactaataa agcaattgcc attgaatttg ttgcagcaaa tccacttggt
     9061 acctttggag ttacaactcc agttacttgt aatattatac ctattaaacc aatgatagaa
     9121 ccaccagcaa caagaccaga agataaactt ataccattag aaactttaac ttctttttct
     9181 ttttcagttt tagatactaa ttcaacaaat aaacgaatta atgctccaac taatattata
     9241 gaagttgttg caattggtaa atagaatcct atagctatag tcattattgg taaatttaat
     9301 gcatatagaa ctattgccat aaatactcca acaataatca taacccaagg taaactacct
     9361 gacataatac cagaagttaa tgttgacatt aagtttgctt gtggtaatgc aaattgtaca
     9421 ttatcaccag ttgctctaag ttggtcagaa agtaataaaa ttactcctac aacaactata
     9481 acaccaacta tacttgcaat tgtaaagtaa cgttgcattt cgtttttgct accaccaata
     9541 atataagtta ctttttgaga ttgagtataa cctccagcta tagcaatagc aacaactata
     9601 aatgaaccaa ataaaagtaa tgatttatta gcttctaaat cagtccatcc cattataaca
     9661 aacactaatg ttacaataac tagagaagct atagtcatac cagaaactgg aagatttgat
     9721 gttccgattg ttcctgttaa acgaccagct actatgacaa ataataaaga taataaaaga
     9781 gatatgatag ctccaataat tgccataaca atgttaccag aaattaagaa tgctgctaaa
     9841 aatccaataa ctactccacc taataaaagt atcatttgta tagatgaacc ttcttctcct
     9901 tctccagcac ttgatttagc tttaagtgtt tcttttatag aagcgattat agttggtata
     9961 agttttattg caccaataat acctccacaa agcatcatac cagcacctat atattttaca
    10021 taactactag atattgctcc aacatccatt tggttaagag gcattgcagg attattccat
    10081 accatagctc catctttagc catatctgta aagtatccta ttaaaggtgc tataccaaag
    10141 tttgataaaa tacttcctgc aaacattgtt aaagaaactt caagccctac tataaatcca
    10201 attcctaaaa gtagaggatt tacttcaata tcaaatttcc atttgtagaa tttgcttcct
    10261 acaagactca ttacattgtt tgctacattt aagaatgaac cagttaatac agtaataaat
    10321 ccacttatta caaatccaat tcccatgtac ttaattgaat ctccaccagc ctctgaagca
    10381 acaagtgttt cagatatagc cattgattct gggtacataa gtttaccatg ttcttcaact
    10441 attagatagt tgtgaactat agcagcaatt cctataccaa ataaaactcc accaactcca
    10501 acagctaagc cttcaaagaa tgtaatttgg cttccaatta atataactgc tggtaatacg
    10561 aaaataaatc cacttgcaac agattctcca ccacttgaca taccttggat taagttttta
    10621 ccaagaatac cctttgaacg agcaaatgct ccaacaaaag cagaacctat tatagctcct
    10681 ggaatacctg cggcaactgt aagaccggct ttcattccag agtaagttgt agatgcggca
    10741 aaaatggcag ctaaaataat accgataatc agtacagcta catttccacc ggttctggac
    10801 ttatctgtga tatatggaac gtaatctttt ccactaacct caccataagc acccttaggt
    10861 aattttttgt tcataaaatt ttcacccctt ttcaatttgt atatattata acatatatac
    10921 atataaaact taacttttta ttattttaca aatagcgagt caaaactact aaaatttaac
    10981 cctatattat taatttctac ttttagatat ttttatgcta attttctttt tatttttcaa
    11041 ttctactaaa aactattaag atataaaata aaaaaatttt ttaatgtaaa attaattttg
    11101 taaaaaaaat gctttaaaac acggaaaagt ataaaaaata caataatcat tcttataaat
    11161 aaattttcac catagtaaat atatacagaa aattcttgtt tttatgcaaa ttgattcttt
    11221 tataaaaata agtgtcataa agaaatgtct gaaatcactt tatgacacct atcataataa
    11281 aaaattattc tggtaatgct cttttattta cttttccgat tggtgtaact ggtatcttat
    11341 ctatcctcat tattattgtt ggaatcatat atggaggtag atgctcattt aaatattctc
    11401 taatagttct ttcatttatc ttaccactat cttcctcatc aattacataa tatgcacata
    11461 tatacatatc tccatcatca tttgttctcg atatacaaac aacactttta atttctgtaa
    11521 tattatttat tatattttca atctcatata gctctatcct aaactcatca atttttacct
    11581 gattgtccat ccttccaata taatctaaat ttccatcatc taaccatctt actaaatcac
    11641 cagttctata cattaactta ttatgttcac aagttttgta aggattctct acaaatactt
    11701 gctctgttag ccctttttta tttaaatatc ctcttgatat ttgaatgcct gatagacata
    11761 gctctcctgg tgctccaatt ggacatagtt tgttcttttt atctaataca tatgcataag
    11821 tattaaatat aggttttcct attggtattt ttccatattc tctgtccaca tgaaaagaag
    11881 tcgatacaac agtaaattcg gttggtccat actcattata tagttcataa ttacgctttc
    11941 tatatccttt taatctttct cctccggtct gtacaactct aagtgaatta ttattgcaat
    12001 tttgtataaa taaccttgca aatgctgttg gcaatgctag tatagttatg tcattttcct
    12061 cacagtattc atttactgta tattcatcaa atttagcttc ttcaggcata atatctattg
    12121 atgcacctac aagcaaataa gggaatagtt gtttcattac agcatcaaaa ctgaaactta
    12181 agtatatacc acatatatca ttttctgtag cgcttataga ttttacgcta taataacaca
    12241 tatttactaa actcttatgt tctatagcta tggtctttgg cctaccagtt gaccctgaag
    12301 tagatatcgt atatgcacaa tttgatgatg ttattttttc agttaattca tatccaagct
    12361 taaacatttc ttcatcattt atatcgagta ctatggtatt ctcatttaca accttacttt
    12421 tgagttcatc tgtagttatc actacttttg cattactatc ttcaagaagg taattcaatc
    12481 tttcttctgg attacttgtg tctattggaa aaaatgctcc ccctgatttc ataatcccta
    12541 ttgctgtaat aattatgtta atatctcttg gtaacattac agctactatg tcttcacttt
    12601 ccactcctat atgatttaaa taacctgcaa gtttatcagt tacttcatcc aattctttat
    12661 atgttaactt tctattttca tccctagcag ctatattact aggtgttacc ttaacttgct
    12721 ccctaaataa atcaacataa cttttatcat atggtatttc atattgatta ttattaaagt
    12781 cttccaataa tactttttct tcttccttac atacaaattc taaatcttct attaaaatat
    12841 cattatcttc tattatttgc tctattatat aatcaaattg atttaaaatt cttctaatag
    12901 tttctctttt atatatagat ggtttatact ttatttttat tttaagtgcc ctcttagtat
    12961 atactattac agataaatca tatccttttt tttctatgtc ataatcatat gataaatcaa
    13021 aacttttagc tataaatatt gtgtttataa gcttagtatt tagatgattt ttattctcta
    13081 tatctgataa caaatagtat ccatactcta aagacctgct ccactgcctt tttagcttcc
    13141 ttacaacatc tataaatttt tcactttgtc taactcttac tcttattgga attatattca
    13201 ttgttttacc aaaaactaca tcatcttcat atatatactt ttgaagtata aggccatata
    13261 tagtttctat gatagagtct atgctaatat tgttcacttt agaatattga attaaccttt
    13321 catgtttctc aatattcatc ttaaaaacct catctactac ttcatcagaa caatctttat
    13381 aaaaaaaatc atttgtaatt tttgctatat tcgtataatc atctaacaaa tccatccaat
    13441 aattcatagc tcgttctaaa ttaacttcat acatatcaaa attccccctt actactaagt
    13501 ctatagaaaa aattcttttg gaattttatt ttcacaatat ataaatatat taataataca
    13561 tatgcaagct ctaaaaacat gagcttgcat aaaattacac ttattatgac tactaaattc
    13621 gtaatattta caatgtttaa aatatattat aacacatata tgattttatt tttctacatt
    13681 tatataatat ataaattaca aatagttatt taactaatat ataacaatac cttttggaat
    13741 atgatatatt atattaatta aatttttata agataagtta tacttatagg agggaaccat
    13801 ataaatgaat atttatgctc atagggggtt tagtggaaag tatccagaaa atactattct
    13861 agcttttaaa aaatgtttgg atatggatat atatggaata gaacttgatg tccacagaac
    13921 taaggatgga aaaattgtag ttattcatga tgaaaaggtt gatagaactt ttaatggaca
    13981 tggttttgta aaagacttta ctttaagaaa attaaaaact ctaaactcat cttttgaagg
    14041 atatcaaagc aataaagaat gtaaaattcc aactttagaa gaagtattaa ttctaatttc
    14101 acctacagac cttattttaa atatagaact aaaaactgac aaaataaact atcccaatat
    14161 agaaaaagac gttttagagc ttattttaaa gtataatatg aaaaatagag ttcttatatc
    14221 tagtttcaac agcaatagtt taaaaaactt tcataaatta gaccctagta taaaaacagg
    14281 attattatgt tatttaccta taaacaatgt agtcaatttt gctaaatttc tgggtaatag
    14341 ttatcttcat ccacctctag ttttagtaaa tgaatctcta attgaacttt gtcacaaaaa
    14401 tctacttggt gtcaatgtat atactgtaaa tgaagagggt gatatactac actgtctaaa
    14461 actcaatgta gatggtattt ttactaacta tccagatatt gcttcaaatc ttttacactc
    14521 aaaacaatac agctaattat acaatagttt gtactccttg acttttatat aattaatatg
    14581 tacaatttta ttaaatttat cttggaggta taactatgga taacttatta aatgaacttg
    14641 aagctttaaa gagtaattta gataatctac caatagaaaa aatagaaaat tatgatttga
    14701 gtaaaactgc tttatttata attgatgtta ataatggctt tgcaagacaa ggtgcattat
    14761 actctcctag agtagagtct ttaataaagc caattgaaat gtttacaaaa aaaatatcaa
    14821 acaaattaaa tagagtaata gcatttactg attctcatac tcctaaatca atagaacttt
    14881 tgagttatcc tgttcactgt ttggaaaatg atgtagaaag tgaattggtt gatgaactta
    14941 agtcgataga aaacctacaa attcttccta aaaattctac taatggattt tttgcattag
    15001 aaaatttgga ttttgataat atagataata taattatagt tggcgattgc acagatatat
    15061 gtatatatca atttgcaata actctaaaat catattttaa tcaacataat attgaaaaaa
    15121 atatagttgt acctatgaat ttagtagata cttatgatat tcctaacgtt caccctgctg
    15181 agattttaaa cttagtattt ttcaacagta tgattcaaaa tggagttaat gtattaaaag
    15241 agattcgtta agcttctaat actttacatc ccatataata caattatcag atatttattt
    15301 ttaataaaat ttctaataga aaaacacagt tttagtgaga agattacaat ttaaaacact
    15361 atctattatt actaatcttc acaatataaa aaatcgcagt ttagatattc cttatacaaa
    15421 tttataagga ataataaatt gcgattttta tttttaagta gtacaattaa tttatactac
    15481 ttcagtattt aaattcattt tttaataatc atacttattc gtagtttcgc acattactac
    15541 ctttattttt ttattaaaca tattcttata ttctaataaa aactcattta gttctgaatc
    15601 cataagattt atttttctat cactacaaag cattatcttt tcttggtcga ttattacatt
    15661 aatatcattt aagtcatttt ttataaatgt tgttatacaa ctctttggtt ggattttttc
    15721 tccatatttc tcataaaatc ctgcactctt cttaaatgac tctaaatctt ctgtagatac
    15781 tttgctggaa gtattattac tcataaaacc tgctgtagaa gggtcgattc caaagtttct
    15841 aagttgtttt tctacttctt cagtgtccat accatatctc tccataaatt gcttttgtat
    15901 tttcataaat ttttcttgag atatgttgtt atctctcata taatcgaata tttttcttga
    15961 aaactcagcc atagacatct ttccatccat aacagaaaaa tataagttat atatgtattg
    16021 ctcaaattta tctacatcag aatctttaac tttgttcttt agttcgttaa agtctattat
    16081 tctatcatca gacatttttc atcctcctag ctaaattttt acttgtataa gattatacca
    16141 tatttctatt atatttatat acacactatt tttttaattt aacgattttt tctgtagcat
    16201 ctaattcgca cattctgccc aaaggaatag ttatcattgg gatacaatgt cctgactgaa
    16261 gattatatat agaaggttta ttgaccttat ttgcaaattc tattagtaat tccatcaaat
    16321 cattttcttc aatacaatcc ttaaaatctc caaaaattat accattacat tcctcaagtt
    16381 ttcctgaaga atatagttgt gttaacattc tatccagttt atattgaggt tctccaattt
    16441 cctcaataaa caaaatttta tcttttgcat ctatttcata ttttgttccc attgtggcaa
    16501 ttattaagga taaatttcct cccattatta ttccctctgc tttaccttca catacagtat
    16561 aaatcttttc ttcttttgga ttttctaaat ataattcatc tttaaattct aaagcattta
    16621 ccaatgattt ataggtaaat ttaccccatt tacctacatt tgctgccatt attccatgat
    16681 atggtaaaag accacaattt ttatagaaag ctaaatttaa acctgttata tcagaaaaac
    16741 ctataaatgg ttttggattt tgctttatta tatcataatt tattttatcc aatattcttg
    16801 ttgttccata acctcctctt aagcacataa ttatatctac atctttatct aaaaacatat
    16861 cttctatatc ccttgctcta atatcatcat taccagctaa ataaccttta taatttaaac
    16921 tacaactttc agaaaactta acttcgtaac cataactttc taaattaaac tttatttcct
    16981 ccaaacttgt atttcttaat ggcccagatg gcgcaattat tcctaacatt taaacacctc
    17041 ttcttataaa atgatatatt cttctccata ctaaatatct ctaatataat tataagatat
    17101 tttatttgta cttgatacat aataatgatt tacataaaat aatataatta atgaagaaaa
    17161 ataaattttt aatatagtta ggatgtgttt tatgcttaat ataaaaactc tttgtcatga
    17221 gataaatgat tgggttataa atataagaag agatttacat aagactccag aacttggatt
    17281 agaagaattt caaactaaga aaaaaattat taaatactta aatgaaatag gtataaatta
    17341 tattgaatat aagaatcata cagggataac tgcatacatt aacgtgagtc caaactttga
    17401 aacagttgct ataagagctg atatagatgc cttacctata acagaagaac ttaattattc
    17461 ttataaatct attaatattg gcaaaatgca tgcatgtggt catgatgcac acactgctat
    17521 tttactgggt acttgtaata tactatttaa attaaaggat tacttaaatg taaatgtaaa
    17581 attctttttt caacctgctg aggaaactat tggtggggct caacttatga tagaagatgg
    17641 atgtcttgaa aaaccaaatg taaaatatat atttggactc catgtaaatc ccaatatcaa
    17701 caaaaatcta attgaattaa aatacaacac tctaaatgct tccaccaata cacttcaatt
    17761 aactgtacat ggttcaaaat gtcatggagc ttatccacat caaggtattg atgctattgt
    17821 tatatctgct catattatta cagctctaca gacaatcgtt agtcgaaaca caaatccaac
    17881 tgattctgtg gttatttctc taggaaagat tgaaggtgga atcaaagaaa atatagtttg
    17941 tgacaaagtg gttataaggg gtactttgag aaccttgact cctgaaacta gagagttttc
    18001 aaaaaaacga attagagaaa tttgcgattt tacttgtaaa acatttggag gaagtatttc
    18061 tgttgaaatt gaggaaggtt atccagctct tatcaattca aaccatctag tagattacgt
    18121 caaacaaaat gctgtagaat tgtttggtga agaaaatatt atccttaaag actcgcccac
    18181 tcttggtgct gaagactttt cgtattttct aagacactgt gaaggagctt tttaccacct
    18241 tggttgtgca aatcgtgaaa aaaacattac ttcccctttg catacctcta cttttgatat
    18301 tgacgaagat tgccttatta ctggtgtcat gctccatgta aaaaatgtat tatcattcta
    18361 actattaata tttttattta ttattttata ttgaccctca catgatgtca tgttataccc
    18421 taaaattaca aatgataaat taaatagttg gaggtaatat tatgaaagaa atattaataa
    18481 atcctatagg taaaatatgt attcaaggcg aagaggtctt tattaaatta gacaaaaagt
    18541 atgtacctgc attaaatgaa ctcgacgatt tcagtcattt aaatgtattt tggtgggctg
    18601 atgggtttga taatcctgaa ctaagaagta ttttagaaac cccaaaacct tataaaagtg
    18661 gaccagacgt tataggaata tttgcaacaa ggtcacctat aagacccaat ccaatagcat
    18721 tgactgctgt acaaattatc aatatcgacc atgaaaatgg cataataaaa ataccatata
    18781 ttgatgctaa tgataatagt cctgttattg acttaaagcc atatactcca agcgttgata
    18841 gggttgaaaa tccgtccgtt cctaaatggt gtagtcactg gcctaagagt gtagaaaaat
    18901 caggagaatt taattgggaa gatgaaattg aattttaaat tacttttttc ttgatttcat
    18961 taacttgttt tccacattta ttttatttac acttaccaag attttatgtc acatatacat
    19021 ataatattag gctgtattat ataatacaac ctaatattat ataataaatt tctatttttc
    19081 ccttaaatca ttcacattct ttctaagcta cataagattt ttctattatt tctttttgag
    19141 tttaatttag cattatgttg aggaaaaaac atttcatgca ttttaataaa ttaaattttt
    19201 cttttattct gcgattttat aataatcaat ttaacttaaa aataatacct aactatttta
    19261 actatgttaa actagtaaat gtaaccactt atatgttaca attttatata aacatcaaat
    19321 ataaaaaact atataaataa atgacacata gtgttatatt tttttatacc aatttagtta
    19381 tttttcagtt acagatttaa taatagttaa tagtaagaca taatattttt atgcccttta
    19441 ttgattatgt attttctgtt atctgttatt atcatcaata ataaacataa gggggtatat
    19501 attatgaaat tgtatttaag tgctgatata gaaggtactt gtggtattgt aaattgggat
    19561 gaaacaaaac ttgaatgtga attttcgcaa cactatagaa cgcaaatgag taaagagata
    19621 acagcagcat gtgatggagc tactcaatct ggtgtacatg aaatacttgt taaagatgca
    19681 catgacagtg gtagaaatat taatccaaat attttacctg aaaacgtcaa aatacttaga
    19741 ggatggacca aagacccact agttatgatg gcaggaattg atgaaagctt tgatgcatgt
    19801 atattcaccg gctatcactc tggagctact tgtaatggta atccactttc tcatactatg
    19861 gatattgatt atgactattt taaaataaat gatgaaatag catctgaatt tactataaat
    19921 gcttatattg ctgcatatta taatgttcct gtagcatttt taagtggaga tgaaatgctt
    19981 tgtgagagtg caaaaaaact aaatccaaat atagtcactg ttcctgtgtc aaaaggaatt
    20041 ggaaatggtt caatttcaat ccatccaaac cttgcactaa aaaaaattga aaaaggtgtc
    20101 aaaaaagctt tatctggaga tttatctagg cacttaataa aattgccaga taaatttaag
    20161 atagaaataa aatttagaga acattataaa gcttttaagg cctcttttta tccaaatatg
    20221 aaaaaaatag attctcaaac agtactattt gaaacagagg actattatga atttttaaga
    20281 atgttattat ttatataaat ttcatatcat aaattaagga gttgattaag ataagtaata
    20341 aaagaaggat tcctttaggc gaatttattt ctgtattttt attttttgct gttttttttg
    20401 gtataacttt ttgtattttc acggttatag gaattggctt cttatcattc ttaggatttg
    20461 aatataaatc tcttggagct gtattaattt tctttttaat atatttttgt ataaccactc
    20521 caattgattt tctatgcact actattttag atatattcag atatgtaaac aaattaccat
    20581 actcaatata taaactttgc gaatttataa ttgattttat actaactttt ctagctatga
    20641 acataattga tacctttatg gattcagtaa cgattccttt aagtacagaa atcttattcg
    20701 ctttactttc ttatctttta tcagaatgta tggatttttt tgatagggat aagaaaaaac
    20761 cttaataaaa aagatagatt ccttaataga acacatattt tcgtatttct attatttaat
    20821 ctatctttat tttatttaat aaatttttat taattaaaat atagtttctt ctatctcctt
    20881 agtcatttgc ttagcactat caaaaaattc ttctatatac actacttcat cactgaccct
    20941 agaatattct gctgcaaatg ccattacatg actttgtata gcttcttttg ctgctactct
    21001 acccttttct agttctttat ttgaaactaa tttaataaat tcagctataa gatttctatc
    21061 atattcctct accatattct gttgatttga tggattcata actatttctt cgttttttcc
    21121 aaattcttta attcttatct catttttatt taaatcccca ccaacttcac catggctaaa
    21181 cattaacttt atagttcttc cattttcttt agtaaatgca cataaattaa aagtagccgt
    21241 cactccattt tcaaactgta gtatatttac catattgtct acaacattat tatcacatct
    21301 gtaaacacat cttccatatg gacttgtctt aagtatattc aatagattct cttcagttgg
    21361 atttatgtga acagctctat ttatacttct atctttttct aaataaattc tctttgctga
    21421 atatggacat tttttttcaa ctaaacactg aagacaattt tgtgccatct ctccactagc
    21481 attttggtta ttaagatgct ttaagcttcc aaatgcagat atttttttgc atctacttcc
    21541 tactaaatat aacaaaatat ccatgtcatg acaactcttt gctaaaatta aagggctggt
    21601 ttcattgcta tttctccaat tccctctagt aaagctatgt gcaaaatgcc aatatcctat
    21661 attttcatta tattgtatac taactagctc tcctaatttt ttgctttcta caatttcttt
    21721 taatttatta taaaatggag aatatctcag tacatggcaa atcataaaaa tcttatcttt
    21781 atacttatca cacaagtcat ctatatgtac cagaccatct aaactattag acattggctt
    21841 ttctaatagt acatgataac ctttttcaag agctagtttt gctacatcat aatgtctatc
    21901 atcatttgta gctattatta ctgcatctgc catcttgtca tgtttaaaaa agtcttctaa
    21961 agtttcaaat acatttttac tatctaggtt atatttttgt gtaaatagtt ctcgtcttcc
    22021 ttttttaggt tctgctacag caactatttc acatacatca gaattttcat aaatatatgg
    22081 agcatatgca tacgttcctc tattacctgc tccaatcaca gcaactttca ctttttccat
    22141 actttaattt atccccttta tattatttat actactctat tatagtctat ttttacagac
    22201 atttggatat ttttaataaa agaaacagca gttataattt ttcaatttta actgctgtta
    22261 taagttaata taccaaacat ttttaattaa attttcttta attttaatac aaagacttaa
    22321 atattaaata caaattataa aaacactttt atctagttta cttcttcttt tctttgtcta
    22381 atttatctaa cttcttattg tataaaaaca tataaaatgc tgccgaaaat actattaaat
    22441 atccaacaac acttaacata tctggataga cattgaataa gaatatactt aaaattgctg
    22501 aaaagattat attactatag tcaaatatag agatttcctt agcaggtgca tatttatatg
    22561 ctagagttat tccaaattga cctagacttg caaatactcc acctagtaat agatatataa
    22621 gttgcataag ggtcattggt tcatatactg caaaagcaat tggccctaca gtaactaatg
    22681 aaaaaactga aaagaaaaat acaattgtat agtaatcttc tctatctcca agaactctta
    22741 aacaagtata tgccattgct gcaaatacag ctcctgcaat tcctccaaga tatggaatta
    22801 cttctacact aaatgttggt tttattatga ataaagctcc tataaatgca attataatag
    22861 ctcctatctg ctttacattt attttttctt ttagagcaat tgcacaaaat attactacaa
    22921 ggaagggact cattttattt agcatattag catctgaaag catcaacttg tcaattgcat
    22981 agtagttaaa tataactcct agagttccaa atattgacct atataaaagt acttttaaat
    23041 tttctttttt accagttaga cttcctttat gttttatgat aagccaaaaa gctattatag
    23101 ctgataccag atttctaaag aacacttttt gaaaacttgg taaatctcca gctagcttta
    23161 cgaatgcaga catcattgca aagccaagcg ctgatgctat tataaacatt atacctttta
    23221 ttcgatttga tttgattgtg ctactcatac gtacctcttt tctatataaa tgtataaagc
    23281 catattaatt aaaaacttag cttatacatt aaataataat tatctaaata ttccaattta
    23341 tactataatt aaaagtgtaa actatgtcaa gtaactaata aatcttttca attaataaca
    23401 ataataatta tagctaaaaa gttatatgtt tttatggatt attatcagaa tatataatat
    23461 aattttatat agtatagact ttttttaatt tattatttag atttttaata gattttttaa
    23521 ctatggaggt atttaaaata tggaaataaa gagatatgaa ggaactggaa gaatgagtag
    23581 agctgtagtt cataacaata cagtttattt atgtggacaa actcatgctg aaggaggagt
    23641 aattgaacaa acaactgaag ttttagctaa aatagaagat ttattaaaca aatatggttc
    23701 agataagcaa catttgctat ctgttactat ttatttaaga gatatgaaag actttgaagc
    23761 tatgaactct gtttgggatg cttgggttga aaaaggattt gaacctgcta gagcttgtgt
    23821 tgaagctaga cttgctagag agcatttgtt agttgaaatg agcgttgttg cagctacaaa
    23881 ataatcaatt tagcttatag gaggctatct caaaacagag agataagcct ctttttcatt
    23941 gttgaaatga tgaaaatata tttgatttcg tataaaagaa agtgtctcac gaactaaaaa
    24001 attcattttg agacacctcc tcactattta atctatataa atttacttat tctctattgg
    24061 caatatcaat ttagatggat atttacctcc atggtaaatc gtattctttg cttctatata
    24121 ttcaatacta ttatatccct ctgtcgtgtt gctattttgg aaaataaagt ttttagcact
    24181 agacgttata tctagtctaa ttttatgacc aactttaaac gtgtttgata tttttgaagt
    24241 tataatcgta aacttataaa tctctccttc ttccataaac tctgacttat aaaaactatt
    24301 tctaaatcta gcactaagta taccatcagc taatttaatt gaatttccat ctaaatccac
    24361 gtccataatt tttataaccc agtcagtatc tttggcagaa cttgatgcaa aaaactctaa
    24421 ctttatatca cctgttacag taaattcttc actaaaagca tctgatgtat aacacaacat
    24481 atcacttctt ttttctaaat ctttgtagtt atttggaact ccaacttcat tctctgacat
    24541 atcaattagt tgtactgatg ggtcttttgg attatatata taactatcat acttttcttc
    24601 taaatcatta tcaaatacta atctaccatt tcctaaagat gtgttagcat ttccatcact
    24661 tattagatac atacttttat ctatcttatt tattattggc caattttctt ctgtcttcca
    24721 cttattagaa cctacagagt aatactctac tggagcagtc gtatctatgt tattttctat
    24781 accttttaag tatttatcaa accataatag ataattataa tccaaatcat atcttaatga
    24841 actatttcct aaagaaatac catttatatc tcttaaagta tttgaattat gcatccatgg
    24901 acctagaata gcctttctct ttcctttttc atagtccttt ataacatcta aggcctctgt
    24961 agttcccata ctattatcat cataccaccc agatacaacc aatgcaggta catttatttt
    25021 atctttttgt aaatgccagt ccatcaaatc ccaatactca tctttttcta ctctctttaa
    25081 ccattcttcc agaaattcaa ttctatatcc aagagcttca acaggtatat tttctattgg
    25141 tcgtatgttt aaaacatcat cccaatcatc tcttaccatt ttgcttctat caactttatt
    25201 tcttgatacc ataaatgccc atgcaagcat accagataca aaagctcctc cttttcttgg
    25261 tatatcaata aaagggcttc ctgatgttac tatactaact aaagctttaa gatgcttatt
    25321 gcctgaagat gcagctgccc attggacata accaagatat gatgctccaa gcattccaac
    25381 tattccactt gaccattcct gtgatactat ccaatttatg gtactatctc catcttccct
    25441 ctcatgaatc ataggttccc acttaccttc tgattcattt cttcccctaa catcttgtat
    25501 tacaacagca tatcctcttt gtacatattt ataatatatt tctttatcat tttcttttcc
    25561 atatggagtt ctcataagaa ttgtcggtgc ttttttggca gaatctacaa aatcagggag
    25621 atatacatct gtagaaagtt ttataccatc tttcatatca atcatataat ttccaccaaa
    25681 tcttatagaa tatatctctt catttttatg agttttttcc cattctttta atacagtata
    25741 ctcttcatat ccatccataa ttaatatagt acaagtttct ctagataaac agataaaacc
    25801 tactatttta ttatctacaa taattatatc tagtgggaac tttttgtttc tttgaaccca
    25861 tatatattca ttaagtttag aatgcgtttc tcctaagtta tttataactg tgtctttatc
    25921 tgtgtttact aagcttttag tatatacatc aaaattatta aataaagact ttaaatctat
    25981 ttttgtaaga tttaaataat atgtattttc ttctttgctt attggtttta aactttctat
    26041 tttatttgta aaaggattca acttttcaat taataaatct ccatcaaaga ttacatttcc
    26101 actgtatagt ccagatatgt ataatttaaa ttttatcaat taaactcccc cctataatcc
    26161 tagagacatc tttattgcat atgccactga tatacctgca taatttccta atactccacc
    26221 aattactccc attacgactg ctacacttac aagcgaattc cactttgcag aagcagctac
    26281 taaagctgaa gtaggaccat cagcaattgc tcctactata gaaagtatta catattgata
    26341 ctttatcttt aaaagtctag ttatgcctaa atggaaaaca aatgaagcaa aaattatact
    26401 aaaacagtaa agtgttatag taaaagttga acctaaaaac tcttttatat ctactgaaaa
    26461 acctattgta cataaaaaga ataatgcaac aaataatcct aaatctaaat ttccctttag
    26521 tttctttact ggctttaatt gagcaattat aattgaaatt gttgtaacta aaagtattct
    26581 tgctgcacta ctaaaacttg aattgaaata tccagcaagt attgttgaaa cttctgtaac
    26641 aacaaaacct atagccaaca tccaagctat atctttaata ctccattctt ttgactccat
    26701 aaattcttca ttttgacaat cttctaattc tgattcttct acatgatatg gccaaagttt
    26761 tttaaacttc tttgaatttt ttaatattgc tggtagagca aacataaata tcaatgttgg
    26821 tactccaatt acatagtctg ctgcatttgc tgctgcaaga gtttgtctac ttacattaag
    26881 acctgttcca atagctgtaa gattagcact tccaccagta tatgtaccta caaacattcc
    26941 agcaaccttc caaccttctg atattttttc tgcaaatact aatccaaata aaaatgccat
    27001 gatacttaca gttaatacag ctacaaaaat tgcaattaaa ggttctcttg ataattttgt
    27061 catttctttt aagtcaacac tcatcaacat tatagtcata gaaattggaa tagcatatgt
    27121 tgaaatagtt ccatataact cagtacttac tggaactact tttaagtttg aaagtattat
    27181 tcccattatg attattgtaa gtgctggtcc taagctttta atggctttgt atctttgtaa
    27241 gtaaaaaccc aatgcaatca ttgatgtcat tacagcaagt atactacctg gatttgaaaa
    27301 aagtgtattc atatttaacc tccaaattta tcataagttt acttctattc ctaaacctgg
    27361 tttattgaca agattcataa tatctccatc cattacaaat cctccagata taccttctaa
    27421 ttcttcacaa aacataaaac tatcaaggtc agcttctgtt atgttctttt tagatgctat
    27481 taaatttgct gctgctgtta taccaattct cgtttccagc atacatccta acatacaatt
    27541 tactccactt gcctcagcta tgttgttgat ttttatagca ggatatattc cacttgattt
    27601 catcagctta atattaaatg tatcaactgc attctttttt atagccttaa ttgcatctat
    27661 tggtgaatgt aatgattcat ctatcataat ttttgtgtta cttttatttc ttatgtaagc
    27721 agtaccatct aaatcccaat gtggaagtgc ttgttcaatt gcatcaacat taaattcttc
    27781 taactttttg atagtattta tactgctatt tacgttccat ccttgatttg catcaattct
    27841 aattcttata ctctctccaa cggcttctct aattaacttt acagcttcaa tatcgtcttc
    27901 aggatttata cctgctttaa gcttcaatat cctaaaacct tcttttactc tttctaaagc
    27961 ttctactgcc attttttctg gcttatctat acttatagta atatccgttt ggactttact
    28021 atcaaatcct ccaagaactt tataaagagg tgcattcata atttttcctt taatatcata
    28081 taaagctatg tcaatagctg cttttgcaga agtattacct ataataattc catccatgat
    28141 ggtatgtatg gtttcaatat ctagtgggtc tttgcctatt aactcttttt taaacataag
    28201 caatactgaa agaacattat ccaaaacttc acctgtaact gctgcaagag gtgctgcttc
    28261 gccaaatcca caataccctt catcagtttc aatttttaaa attatacttt ctccatactc
    28321 aattactcca aatgaaacta ctacaggttt ttttaattta attctaagct tttcaaattt
    28381 gatatctgtt atcttcatat taccctcctt attttttgaa gttataattc aatttttaaa
    28441 attttatact ttaaattata ttattacttc aatatatatg tcaagataat ttactgaaaa
    28501 tttactcttt tccaaaatat aatgtgataa tcttaattca attaatactc ttctattaaa
    28561 tttttttatt attattttct ttgtattaat ttaacaataa ttcattttca tagactttta
    28621 tttggattta attaaatttt aattgtcaaa atctactatg tattcttatt gccactactt
    28681 ctatgttgtg ctataattac tttgttttaa gcgaatattt tatattaatt ttattatttg
    28741 gaggttctta tgctaggtga aaagatgaga aatataagaa aaagcaagaa aaaaacatta
    28801 agtgatgttt ccaaattgac agatttatct ataagttata tctctcaaat agaaagagat
    28861 gctattgaac catccttgtc ttcattgaga aaaatcgctg aagttttaga cactccttta
    28921 tatatgttta tggatgacaa taatactgat gatttagtca ttagaaaaga agatagagtt
    28981 atgatgaaat ttccaaagag tgaaatgttt tacgagatag tatctccaat gcctacaact
    29041 gagtttacac cttcaatttt atttgtagag tttgaactta aaccagaaag tgaggatgcc
    29101 aaagattata tatatcatgc atcagaagaa attatggtgg taacgagtgg tgtggttgat
    29161 atttgtatgg gtgaaaaggt agttagatta aatccaggtg actcaacatt tataaaagct
    29221 aatgtaccac ataaaatcat caatccaagt aaagatacaa tagctcgtgg atatggagtt
    29281 atatcacctc caatttggcc aataaaatct aaataaactc aatatgacac acctactttt
    29341 tgtagataat ataatagatt taaatattgc ttttatttta aatttaaaat atttactcaa
    29401 actaaaaaaa tagctatttg actaatctaa accatcttaa ttatggtaaa tagtaaatag
    29461 ctatttgtaa gttatattat aaattataat aaaaattgta tttgttaaaa ttaaactgct
    29521 atatcattta atacttttgc ctttacaact cttatatttt tcatactctt gtagatatct
    29581 tgaatcttat cttcttgctc tctcaatagt ctatgtgctt catatatatc tacaagttca
    29641 aattttacta catcatttgg ctttgcttgt gctaacttat acaaatctac acttataacg
    29701 ttccctatct tagtatagcc accactagtc tgtctatctg ataacattat aatcggtttt
    29761 ccatgaccag gtacttgtat agctccataa gaaataccat cagatataat atctgctcca
    29821 ttaaggtgct ttatactctc tccatctagt ctacaaccca ttctatctat ttcattcgta
    29881 actttgtatt tatttgataa aaatttctct acttcaccgt tagaaaaagc atcaaattga
    29941 ggacctttta caattcttag tacgatttct gaactataag ttggaatata ttttgaactt
    30001 aattttttat ttattgtaaa atctttttcc tgaataaaag tatttatata atctcctttt
    30061 ttaagtgctc taccttcaaa tccaccaatt ttagctcttg tataagttga cttacttccc
    30121 attgcaagtg gaacatctat tccaccagct attgacacat aggctctagc acctgatttc
    30181 acacctctaa atgataatac atcacctgga tttatttgat atgacctcca gttttgaatt
    30241 tcttttccat ttattttagc ccctaaatct cccccagtta ctgctatttg cgtatattca
    30301 tcaaaggtaa ttgtaggacc taacattaaa acttctaaag aagcctcatc ttcagaattt
    30361 cctactaata tatttcctac tctatgtgca tactcatcca ttgctccaca tccagataca
    30421 ccatattgac catagccaac tctacctctg tcttgtatta atgtttggaa acctcctaat
    30481 tcaactttga atcccatata taccactccc tgtactataa cgatatttaa atacttaagt
    30541 tatttttttc gatttctata aattcatcta atgttattgg aacaaatttt ataaaatctc
    30601 cagctctaag caaaatctgt tcttctctat tttcatcata taattttaaa ggtgtcctac
    30661 caattatttg ccatccacca ggactatcaa taggatatac accagtctgg gaacctgcta
    30721 ttccaacact cccaccataa attttagtcc ttggcacttc taatctaggt gtagctattc
    30781 tttcatccat accacctaga tatggaaatc caggagtaaa acctagcata tatatcaagt
    30841 actctctgct tgtgtgtatt tttataactt catctacagt gagattattg tagcttgcaa
    30901 cgtttttaat atctatacca aaaacctcat cataacatac tggaatctca tgtattttag
    30961 gttttggtaa tactatactt tccaaattat cttctatttt ttttaaattc tcaatcaaac
    31021 tatcaaaatc tatttctaat ggattataac ttatcataag tgacctatat gttggtacca
    31081 tttctgttac aataccttta agattcgata tttctattgc tctcataaat gaaattactt
    31141 ttttgtttat atcttctgat atctcatttc caaattcagc aaccactgcc ttatcccctg
    31201 atatcaagta tctagtttcc ataaatgtca cctcttctaa attaagcaaa taaagcacct
    31261 aggctgctaa gagaagttat tcctgtatat gcagatatta aaactactat taatccaaat
    31321 attaaaagcc atgtaggatg cttgtaatct ccaacaatat cttttctttt agatgctata
    31381 agcataattc ctaatgtgat tggaagtatc aatccattta aagctcctgc taaaattaat
    31441 aaagtagctg gttttcctat aaatgccatt ataagtgttg atattgctat aaatgctatt
    31501 ataaaatatt tttcatattt atctataaaa ggatttagtg tctttaagaa agaaactgat
    31561 gtataagcag ctcctataac tgatgtaatt gcagcagacc ataatacaag tccaaagaat
    31621 ttatatccta ttgctcctgc actaaattta aaagctgaag cagctggatt ttctgggtca
    31681 agttgaagac cttttgaaac cactgccagt attgctaaaa ataaaagaac tctcatcatt
    31741 gtggcaacta atatacccaa aagagaagat ttagttattt cttttatatt ttcttctcct
    31801 gttattccac cgtctattaa tctatgtcct ccagcaaaag ttatataacc tccaacactt
    31861 cctccaagta gagttattat tgggaatatc agtccttttg gattttctgg ttttaccatt
    31921 cttgatactg cctctccaac aggaggatga tttgatattg ctacatatcc aactataatt
    31981 atcattccta atgctaaaaa tttagttatc ttatcgacta gagaatttga attttttgac
    32041 ataaatacaa atatggctat taatccactt aatacagttc ctaatgtcat attcatatta
    32101 aacattacat tcataccaag tgcagctcca ccaacatttc ctatattaaa agctaatcct
    32161 ccaagagcta ctaaaaaagc tactaaataa cctaatcctg gtattatttt atttgctata
    32221 tcctgacctc taagtcctga tacaccaatt actctccata catttacttg agcccctata
    32281 aaaaggattg ttgtaattaa aattacaaat gaaaaattcg gtccaaaatc ttgggtaaat
    32341 tgtgcagtct gagttaaaaa ccctggtcca attgctgatg ttgccattat aaatgcagcc
    32401 cctataagtg ctccgacatc ttttttattt ttagttttat ctgtaatatt ttttgtactc
    32461 atattttatc tccccctcat gttatcagtc ttatactatg ttctctgacg aacaaacttc
    32521 tatgttttct aatggacaaa cttctatact ttctaattca aaacgctttc ttatttcttt
    32581 gacaaactct attgcttttg gattgtcccc atgtacacat atagaatctg ctttgatatg
    32641 tataacttca cctgttaaag tttctacagt tccttctttt accatcctaa caaccctatc
    32701 tattgcatgt tttgtatcat gtataactgc tccttctaca tttcttggaa ccaaaaaacc
    32761 attattatca taagctctat ctgcaaatac ttcatttgca aacctaagtc caatttcttt
    32821 agcagaattt atcatttcac tattatatag ccccaacagt ataatatctt tatcgacttc
    32881 atatatagct tcacaaatcc ccatagctaa ctctttatct ttagctgcca tattgtataa
    32941 tgctccatgt ggttttacat gttgtatatt acatccattt gaacttgcaa atgccattaa
    33001 agcacccaat tgatattttg tataatcttt agcttcctgc tttgttattt tcatctcacg
    33061 tcttccaaat cctattaaat ctagaaaacc tggatgtgcg cctattttca ctccattttt
    33121 tttagctagc tgtactgttt tttccatatg actagggtca cctgcatgaa acccacaagc
    33181 tatattagct gatgaaatat attttaaaac ttcttcatct aaaccaatct tatacgttcc
    33241 aaaactttct cctaaatcac tatttaaatc tactttatac aatttatgac ccctccttgc
    33301 taacattttg atagaataag tagttttaat ttttaaattt tgtttatttt atatattgca
    33361 tgaaatattt tatcacttgt attcaatttt atttttgcaa ctattctatt gcatttaatt
    33421 aatataattt taccatttaa ggaatatttt tgcaattatt ttaattgaac attcatttgt
    33481 atttcttttt caaaaaatct ttttaccatt ttttagttaa taattaatta ttttcactta
    33541 aaataagggc tttaaccttt atttttaaag tatttttcat ttttaatcac tttatatgca
    33601 agacagaatc atttttcttc attcatttgt ttctttcatc tatataatta tcattctttg
    33661 taagtaaaat atctttatat ttttataatt aattctttat atagttttaa gtataataaa
    33721 atcatgtata ctaaatgcat gttaaaatat tattttaaaa catgcacaat atttgtacat
    33781 attgtagaca tataaaccta taaattatac aatagtattg ttaagattat atattttttt
    33841 aacataaatt ttgtattggg ggaaaaacaa tggataaaaa ctatacgatg ccaatatacc
    33901 aaaaaatagc gttggatatt gcaaataaaa tatacacggg tgaaatacaa gaggattcag
    33961 tactatttgg acgctctgtt ttggctggta aatacaatgt atctccagaa acgataagaa
    34021 gatctgtcaa aattttagag gacattggcg ttgttaaaag cataaagggt aaaggtgtta
    34081 ttgtactatc tccagacaaa gcttcttctt ttataaaaaa atatagagat attacaaata
    34141 tatcatcata taaatcaaca ctttataatt taatagatac taaatctaat ttagaaaatg
    34201 aaatattaga tactataaat aaaattttag actattctaa cagacttgaa ataatcaatc
    34261 ctttagtgcc cgttcagttc accataaatt ctaattgcaa atatattggt caaacagctg
    34321 cacaaactaa attttggcaa aatacaggtg ctacaatagt tgcaattaaa agaggtgaag
    34381 aacttataat atctcctgga ccatatattg aatttttaga aggagacatt ctcttagtag
    34441 ttggtgacca acacatttac aattctatac caatgttttt atatgaaaac gaaaaataaa
    34501 ttatataatt tagtatgatt atataaaaat gcaaaattaa caacaaaaaa agagcttcat
    34561 attttacata aagctctttt taaattcaaa taaatttaac tttgattaat ttttaaatat
    34621 ccaattcatc ttctttttcc cagcttaaag ctctcgatac agcttttttc catcctttat
    34681 ataatttaca tctcttctct tcactcatat ttggattaaa ctctttttct actgaccact
    34741 ttgaagtaat ttcatttctt ccaatataga atccaacaga caaccctgct aaataagcag
    34801 ctcctaaagc tgtagtttct actattttgg gtctatctat atctacattt aatatatctg
    34861 attgaaattg cattaaaaaa ttattattac ttgctccacc atcaactttt aatgatttta
    34921 gttttaattt tgaatcgttt tgcattgctt ctaaaacatc ctttgtctga taagcaattg
    34981 attccaatgt ggctcttact atatgttctt tttttgctcc tcttgtaagt cctaaaattg
    35041 aacccctagc atacatatcc caatatggag ctccaagacc tgtaaatgct ggcactacat
    35101 atacaccatt agtatcctct accctatttg catacatttc actatctttt gcagattcaa
    35161 ttatctttaa ttcatctcta agccattgaa cagatgctcc tccaataaat atacttcctt
    35221 ctaatgcata tgttactttt ccatcaactc cccaagcaat tgttgtaagt aacccatgct
    35281 tagatttaac tatgttactg cctgtattca taagtaagaa acaacccgtt ccataagtat
    35341 tttttgcagt accttcttca acacaatttt gaccaaataa tgctgcttgt tggtctccag
    35401 cacatcccgc aataggtatt ttagctcctg ataacatgcg ctcatctgta tgaccataaa
    35461 catagctaga aggttttact aatggcaaca tgcttattgg tatgtccaat tctcgtaata
    35521 tatcctcatc ccattctaaa tcatttatat tgtacatcat tgttctagat gcatttgagt
    35581 aatctgttac atgtactttt ccacgagtta aattccatat taaccatgta tctatagttc
    35641 caaataataa ctctccttgt tctgcttttt ccctagctcc ttctacatta tctaatatcc
    35701 attttacttt tgttgctgaa aagtatgcat ctattaaaag acctgttttt tcttttattg
    35761 attcttccaa tcctttattt ttaagttcat cacatatttc tgacgttctc ctacactgcc
    35821 atactattgc gttataaata ggtttaccag tatacctact ccaaacaacc gtagtctctc
    35881 tttgattagt tatccctatt gaagcaattt cttctggtct tatacctgct gtttctataa
    35941 cttctctcat taccccactt tgtgaccccc atatttccat agggtcatgc tctacccatc
    36001 cagcttttgg ataaatttga ttgaactctt tttgtgcaat tttcactatt tcgccttttt
    36061 tattaaatag tatagctctt gaactggttg ttccttggtc taatgccatt acatacttct
    36121 tttccatcac catattctcc ttatcatata atatttaatt tattaaaaag aaaaatttaa
    36181 tatcacatat ttcaataaat aatcatactt aagtacaaat ttaatataat agatatttag
    36241 taagaagaca cttattttct atcatatcta tataataata ttatacatta tagcttacat
    36301 attgattaat aaattttaaa ggagtataat aaacagtgtt cataataatt ttaataaaag
    36361 aggtggtaat atgccaaaga ttttagaaaa tgtaaaagaa gatatattaa aagtgtctcg
    36421 ggatatgatt ttagaagaag attactcgaa tattagcata aggaaaatag ctcaaaggtg
    36481 tggtataagt gcaggtacag tgtataatta ctttaattca aaacaagaaa taatagaata
    36541 tatcacaaaa tctgaatggg atttattgat aaggcgaatt gaatactcca ataaaaacac
    36601 tgaagattac ataaaaaaac taaacattat ttttactgaa ataagaaatt ttataaacaa
    36661 agttcataat atccaataca atgacttttt aaacactttt gaaacagaga gattttttga
    36721 aatgaaaaaa cacaaagacg actttcatga acagctttca aataaagtat atgaagcttt
    36781 agaaaaagaa agttttttta ataatgacaa attagtatgc aatatcataa taaaaatgtt
    36841 tttttcatat tcaacatatc ttcatataga atttgaagat ttaaaaccct atattgaaaa
    36901 attaatacat taaggtggtg tttaccttga atcataatac tagtattaag aacaaggaca
    36961 gtaatccaaa aatattttta gtacttacac tttatctatt aggtatattt atgggagcta
    37021 ttgatactgg aatagtatca ccagcaagaa cgattataca aaatagcctt ggagtaaatg
    37081 aaaaaacggg aatttggatg attaccatat acacattaac atacgcttct gtaattccta
    37141 tctctggaaa attagcagat aaaataggaa gaaaatatgt gtatctagtt agtatatttc
    37201 tatttggtag tggttcatta atatgtggat tatcatcact attttccaat ttctatattt
    37261 tattaattgg tcgtgttatt caagcaattg gtggtggtgg aatcatgcca attgctacag
    37321 cagaatttgg aactaccttc ccagaaaaca aaagaggcat ggctttaggt ttagttgggg
    37381 caacatatgg tattgccaat attttaggtt caagcattgg tagcacaatt ctaagtatat
    37441 ttggaactca aaattggaag tggctatttt ttgtaaatct tccaatttgt ttaatcatac
    37501 ttattggtgg agttttctgt attaaaaaca ataagtctga atctaaagaa aaaatagata
    37561 agcttggtac attgatgttg atatgtataa tcgtatcact attatatggt cttatgaata
    37621 tagacttttt taattttaaa aattctatac aagatatatc agtttatcca tatttattga
    37681 catttattgt acttatacct attttcatat ttattgaaaa tagagctaaa gaccctattt
    37741 taagctttga atatttttta aatccgagaa tattgatagt acttatttta tctttaattg
    37801 ttggtattgg aatgatgggt atggtattcg ttccacagta tgctgaaaat gcattaaaga
    37861 taaatgctgg ttctggtgga tattttgtag ctatattggg attatttgca ggtattgctg
    37921 ctcctttaag cggaagactt attgacaagt ttggggcaaa aaaaatacta ttactaggat
    37981 tttctgtatc tatgataggc tctctttacc tagttttaat tgctctaaaa accaatactg
    38041 tatttagtgt atgtgtaagt ttaatattta tgggattagg aatgggattt actatgggaa
    38101 ctcctctaaa ctatatgatg ttatcaaata caagattaga agaatctaat tcagcacttg
    38161 caacattatc cttgatacgc tcaattggta cttcaatttc tccagctata atgattggat
    38221 ttatagcaca tgcaggatta tctgtgcaag ataatattat ggacttagtt ggaaagccct
    38281 caactcctaa aatagttcaa cttgaagaac ttgaaaacat gatagatgac ttaaaatcag
    38341 accctgaaat ggcaaaaaat ttaaaaaatg tctctatccc aaatatgaat gatacttcaa
    38401 atataaatat ggacatgaca ggtggcaaac ttccaaaatc cttacttaat aaagttcagt
    38461 cagcagatac aaccaacatt accaatataa caaaagaaat atcaacaaca atgtttgatg
    38521 aaaaagttcc atctataatt actaaaatac agaaaaatgt acaaaaagga atagatggaa
    38581 cacaaagtgg aataaacggt attgaagatg gtgaaaataa actaaatagt ggtattaaag
    38641 gagttcaaac tggtattgac aatatggtaa aagctagagc tggtattaat caaggaatag
    38701 atggtgtaaa aaaaggtatt gctggtgttg acaaaggtat aaaaggtatg gaacaaggtc
    38761 ttgaaaaaca agatgaggcc atcaaggaac ttacaactgc ttataataaa atacctacta
    38821 cagaaaaaac tcctactgat aacgaaaata actcaaacga acaaaaccag agtaatacca
    38881 gtaattctga atcagatgac actaagaatg attctacaga aaataagaat aacaattcca
    38941 gcgagaataa accaaatgta aatgaacaaa aagaaagttt aaatgctcaa atacagcaac
    39001 taaaaaagac aagagatgac ttaaatacaa agttacaaaa aaataaaact caaaagaacg
    39061 aattaagtaa aaaactaaga tcaatggaat ctcaaaagaa agagctacaa agcaagttaa
    39121 ataattctat aagccaaaag aaagacatgg aaaaaagcct tgatacaatg caacagcaaa
    39181 aaaaatcact ccaagcagtt ttagcaaaga cacaagaggt aaaaactgaa ataccaaagg
    39241 cttttgataa atcaaaattg gattatataa attccataga aaataataga acaaaaatag
    39301 aaaacacact acaatctact ctaaactcag gatttaaaca gatgtatata acagtattct
    39361 gtgtaaatct actagcattt ataatattat tgttctataa agaaaataaa actagataaa
    39421 agagtgttat agtattatat tgtaaattta tcttattctt aaatatttta ttctaaagaa
    39481 taaattcata aaatttttgt aattgattaa tataaattac aatataatac tatttttctc
    39541 ttaatctttt tctcttttac agacaatcaa aaagcaatct tagtttatcg aaataaattt
    39601 atacaatcta ttcatacacc cctttacatt tatataacaa ttaaacctat tgttatatat
    39661 acaaatcaaa ataaaccata tattttcaac tataatatgt taaaatcata ttatgagcat
    39721 attaatttaa gcaaaatgtc atttagactt taatttagta gttttatatt ataaatttat
    39781 atatttttaa ttttaaatgt catttagaca ttactcataa aaagaaagga gtgtattttg
    39841 tggatacttt aggaaaaaga attgcttatt tgagaaactc caaaaaactt actcagcgca
    39901 aacttatgga tattttaaaa tttgaaaact taggcaagta tgaaacagga gatagaaaac
    39961 ctaattgtga tatactaatg tcaatagctg attattttaa tgttacgact gactggcttc
    40021 tctatggtaa agagaaggta aatgtcaata gtagtgtaaa agaagataaa gaagattact
    40081 tacatgttac taatgatgaa atgacgatat taaatctata cagacaacta aacgaacgtg
    40141 ataaaataaa aatagaagga atacttgaac ttaagatatc tgaatataaa gacttaaaaa
    40201 agcattcctc taataataat gaagataaaa tggtttaaac atacataatt tgatacgatt
    40261 ttacatcatc tattaacctt ctttttttgt agtatattac taaaatatgg atattggatt
    40321 atttaaaaac aagtatagtc taatttcaag cacatttata atatttagat tatcttatcc
    40381 atattttagt ttaagtactt tgaaaaatat aatacatctc tatgtataac ttatattcct
    40441 aagaaatgca ttactcatgt ttttaattct tccaacatct ttctatacac aattgtacat
    40501 ccttcataat tcccctcttt aagaaggatt gaacatgtac tagaatattt agttaaagta
    40561 tttaatcttt ccatatttaa gctttttata ttactgagtg ctgtaataaa atcatcaatt
    40621 gctctacaac actctataac cccatctgaa tccaatttac acacaagaat attcacctac
    40681 tttatatact atttattaat catcttctga atattctata ttaagtaatt gataaattga
    40741 tgatttatat tttttacctg ttcttcttcc atttaaaata gctgacaaat agttttcatt
    40801 aacacctatt ttcttcgcta attccctttg agtcatattt aattcaataa gtctttgctt
    40861 gacttgcact cccaaaaaag gtatccattt ttttcgagca tccatttatc aacttccttt
    40921 ctaagcgatt tatatttact tttatattat tttagcttac gcaccccctc tatttagtat
    40981 atatttttaa tatgtacaca tattaagtat ataaatttat ttaatactaa ataagatttt
    41041 ttgattactt tgtgctaaaa ttaggatgta aattcgtatc attaagttaa ttataaagtc
    41101 attttgacct taagtcaatg gtttttatgt cattttgacc tttaatttga aactatttac
    41161 atcttaaaaa gtctttttga cattaattga atggagagtg tttatggata ctttaggaga
    41221 aagaattgtt tatttgagaa aagcaaaaaa ccttaaacaa tatgaactag aagaaatgtt
    41281 gggctgtgac aacttaagta aatttgagag aaacattaga aaaccaaatt atgaaatatt
    41341 aaaatctata gctgagatat ttaatgtttc agtagactgg ctattaaatg gagataatct
    41401 atcacataaa tcagatttaa tttgcgattc ctcctcaaac tatccactta atagcataaa
    41461 ctctaatgaa ataaagcttt taaacaattt tagaaaatta aatgattatg ataaagctaa
    41521 aatagaaggt atgattgaat taaaacttca tgaatatgaa aaagagaagg atttaggaaa
    41581 aattgagtat aacaaaaata aagatgagaa aatagataaa taatttttat gttgcttttg
    41641 atacgatttt acatcatttc tttgttctaa tatttttatt tcatttattt tcttttttat
    41701 aattttatga taaaataatt agtatatttt gctttacttg aatgtaatta tttatacaaa
    41761 agtcaaacat aatataaatg tagtaaattt ttagtttcaa gtaatatctc gttattataa
    41821 ctaaatattt attaaaatta ggaggataaa atgggcttaa gagacaaatt cgcgcaatca
    41881 tttgctagat caaaaactat gagtggccct gagaaaaagg ctaatgaaat aatgggtaaa
    41941 ttgcttctta agaaagctat attacctatc gttttaatgt tcgtgataat aatcgctgga
    42001 gctatgctta aaatcaattc ttgggttaca cttggcataa atttagttat tgctgttggt
    42061 gctttcttct acataagaaa ttctagtaaa aagtatcaga actttaaacc ttatgttggc
    42121 aatttgatta gtttagagaa aaaaggtaaa aaagagtacg ttgctattat aaaacaaggt
    42181 aagttaccag ttaagttaca aatagcttat ggtggtgaag accttgaaca tgttaagaaa
    42241 aatcaaatgg ttcaaataag ttataatcca gatgctaaaa tagctatttt ggttaataga
    42301 caataattta ataaaaaaaa ggagttgtct taaaattgat tttttctaat caaatttaag
    42361 ctcaactcct tttttaattt ttatatgtta tttttattac tttttttaat taatatttta
    42421 aatattaaat ccttctcaat aaaatagtga actatctaca atactacttt atcttttcct
    42481 aacaatatat aatctggtgc tactctttca tgttatacac cattttgtaa catacctttt
    42541 atatcagtaa catcttcctt aatttcctct acatctgttt tcattatacc catttgaaca
    42601 agtatatttt tgtttatttc ttgttgttgt gtagataatt ctataaaatt ttctacagtt
    42661 tttttataca tgtctctatc ttcttttttc tcctgcatag tatttttaaa taatagagca
    42721 catagtatac ctattgcccc tagactactc aattcggtta ttaatttatc cattatagcc
    42781 tcctattgtc taaatttttt atacttatag ctttaatttt tgttgtattc ttacatcatg
    42841 caatattata ttttcaggat tatcaattta atcttctgta ctttaaattg taggtgtaat
    42901 ttatagattg aataaataat aaatatatat acaagttcta atgtaacaaa aaataagtac
    42961 aaggcttact tattaaagtt aagaccttgt actttacata aaaaaaataa ctaatacata
    43021 gttattctaa atttactatt caactggtat ttctaattct ttacatctaa ttctaacttg
    43081 ctctctaatt actgctggta cttcttctat tgtttttttg cctcttaaaa ttaaagctac
    43141 atatacttct accattccac tctcacctcc agattttctt acactaaaag tgcttatatt
    43201 acttgcttca ttgacagaac tttgatttaa caacatctca tatatttcta catttgctat
    43261 aaaattattt gttatatcat catcttgctt tacttgacct atacttatgt ctgaaatgtc
    43321 tttatactgc atttctactt ttctaccttg tttgatttgt tctattttat ttttatcgaa
    43381 tttaaacaat tttcacacct ccattttaag cttgtggctc gttgttttca gcatcatatc
    43441 taaaaatcat agatggacgt tttatagctg tccatttatc tcctttttct actatgattt
    43501 taggctgagc actttttatg ttggcttctt ctaattcata gatttcttgg taacttgagt
    43561 ctgatttttt gatttcaact agtttcatgt ctttgaataa gtcagattta tttattttta
    43621 cttgaacttt agttttattt attaattgat atataggatt gttttccatc atatcatcct
    43681 ttaatccctc atttaatgtt actattaagt cagtgttatt ttcagtaatg tttatgattg
    43741 aatatacttt attgtttatc attaaaatag aatccttttt tactttaagc cctgtttctg
    43801 atttaaatat aaacatactt gaatataaat cgcctgtagg actactggct ttaatcagta
    43861 tcttattttc tcctaatact attttatttt gtggtatctc aaatataatg ctatttattt
    43921 ccaaattagt cttagtagat attaattcat catttataaa catttcaagt ttttctataa
    43981 cattattagg gtcttgtact acaactctca cttgagatat tgatttttca actatttcta
    44041 tagatgcttt acttgtatct acttttgtta tcatgtatat tgtatcatct gaaagctcta
    44101 tatctaaaat atcatttaga tgttcaggaa ttggtatttt agtaaattca tataaatcag
    44161 taattgatat agatatttta tacatagatt tatttttacc agttatccat agactattat
    44221 catttttaat tactagtatg tggtcatcat tatgtgactt aatttcttta acattagtta
    44281 atactttagt aaaagttctt attatcttat ctttttcact ttctcctagt ccaaataatc
    44341 catttgtatt gtacccttga gcgtacaact ctccctcatt agttaaaaca agtgtcattg
    44401 agttatatat acttatatct ttaacagaag gtaatccagt aaccttttgt aactctctta
    44461 catcattatt gtggccaact cccaattggt actcagtatt ctttccatat gaccatagag
    44521 tattatctgt cttaataata taggagtagt tatctccaca agcaactttt ttgacatctg
    44581 atatatttac ttcagtaaga gttaataatt ctatatcttc aagggtttga ttaatttctt
    44641 tatatgaatt acttcctatt ccatataact tgccatcctt agcaacagcc aatgaatgtg
    44701 tttttgatat atcaatatca gatacctcaa atggcaatat tattgaagta tacttaaaat
    44761 tatctttaaa tatgttttta taatttatat acttattatt tgtttttata tacttatcat
    44821 taaacattac tagcttttca tttttcttat taaaaactat tttatctgat gtgttatctg
    44881 ttttaaaata tgaatgtagg gaaccttcaa gagcttcatt tttatcatta tcatatctat
    44941 ggtgaatacc taaatgatta tatctattat ctcctgttga agaataaaat tttccatata
    45001 tatctttaat aaatatagta ttgtaactag aaaatacagt atcaacatct ttctttatag
    45061 atactttata atatggaact gacatatccc tataagattc atcagatata ccataagtat
    45121 ctctacctga tgatatcaaa ttgttattat tatctatata aagtgtatta ttagctgtta
    45181 caaaaacatc tttaacatct tccacactat ttgtattatt attatttgct atacttccat
    45241 atccactaag acctccaaca aatagtttat tgtcatttgt taaaatggct gaagtatatc
    45301 cattaactac tatttttttt gcatttataa tattattaat tactcgtatc ccctttatat
    45361 cattagtctc tgttgaaaag aataccctct cttttttccc tgtagtataa atcatattgt
    45421 catttgttat aaaaaatgta gcttcacttc ctgctgtcac aacagatttt acattctcaa
    45481 tatttatttt tgtaaatgta cttatacttc cttcagtaaa cccaaaatag ccatttacaa
    45541 gtccacatac atagcatgta ttatcatttt taacaatgat tgatacatca ttacatagat
    45601 atacatctct aacaccattt ataggtatac gtgtaaatat attcctactt gtagtatcac
    45661 ccaatcctaa ctgtcctttt ccattattac ctgctccata taaaagacca tcattagtta
    45721 ataatagaga tgtgtttcca tatacagcta catattttac attttcaaca tttattttag
    45781 taaatatatt tctatttaca ttatctccta ttcctaactg accagaatta tttaaaccaa
    45841 cagcataaca ggtattatca ttttttatcg caaatgcatg tttactattg tcagtaaatc
    45901 tatttacatt tatagatttt atattatcaa tatttatttt agtaaatacc tttctattta
    45961 tctcatctcc cactcctaat tgacctacat catttaaacc aacactgtaa agcatattat
    46021 tattagttaa taaatatgta gactttgaat ggctatatat ttcttgtata ttagaagata
    46081 tttcactact gctattcttt ttattgtaat ttatcttatt atttaaattt aaactactat
    46141 taactgatat accatgcgta cccttagagt tatatccaca tataaataag ctcttatcag
    46201 ttaatattgc aaatctagtt ttatctctac ttaaatcata aggaggtata aatatatttt
    46261 ttatattttt cattaccttt ttataattag tggcattata tccatcttgt gtatctgaaa
    46321 catctagttc ttttgcactt tcagatatat tcccaaaacc atatagcgtg ttagaagata
    46381 aatacaatag agctttatat aaaaatatgg ctttatcaga ggtaatagga cctgaattta
    46441 tattagatgc tgcaatatta ttagatgatt gattgttaag ataattcctt ccatattgat
    46501 gtatataaga tgaacctcca gaaataagta aattatctgg attgacattt tttaaattac
    46561 ttgacgattc cccattatat aaataagcaa aagttgtaga agatgatgat tgtgtacatc
    46621 ttgtaggata ataattatat atataagaat caagatatcc tgcatatgaa attccctgat
    46681 attgaactct atagcttgaa tcagttggtt ttgtccatga agtaccatgg tatccagtta
    46741 gaaaagatcc aatgtttgta caaaatacag ttacatactc atatatgata atattattaa
    46801 ttttagtaat attattcgtg catgaaactc tactataact tgaccctgat atatttatat
    46861 aataccaatc acttaataaa cctagattta cattagatcc atcagaagta ctaaaaccta
    46921 acaggtaatt tgtatttata ccagttgcat ataccttttt ttcatcaact acaataaatg
    46981 ttgcattata tgaactataa gcatcagtta cattactaag gctaatctca gtatattgac
    47041 ttattaatgc attactaagt ccattaacat aacttgaact ctttccagaa gtgtatattt
    47101 gcttattatt agttacaatt aatgaaaacc agtttgataa atcagtattt ataacaactt
    47161 tttttacatt atctatagat attttcttaa atacatctga attagaagga agaacacctg
    47221 ctattgagtt actatttggt ccagaataat aacaagtatc atcattttta atgtagaata
    47281 ttcctccaga aatatttttt gatgctacta tagactttac attagagata cttaattttg
    47341 tcaactcatt taattgactt ggtaagtcag aacccattcc tttgtatcta ttatttccta
    47401 gaccatatac atctccattc cctaaaataa ccagagtaca ataaggacta aaaacgacat
    47461 ctcttacaga atgtatatct agtggaagtt caatatcctt tatttccatt tcttctttta
    47521 ataaagttaa tttatcctca gatttaatta aatttatata attactataa ctatattttt
    47581 cactatccat tttcttaaat tctacaatgt gcatatcatc aaaaaacgaa cctaattgtc
    47641 cataagtatt atctcctgta caatatgaat cattatttga atcaattagt atgctatgtg
    47701 ataaaccaat gaaaatattc ttagctacaa tattttttaa atctataaat gaattatcta
    47761 tattctcaga gtaatttgct atacctaact gatatatatc attatttcca gcaaccttta
    47821 aagtaccttt gttatctata agcatagtgt taccatgacc acatgcaata gtttttatat
    47881 ttacttcttc tatcttagta aatagtgatg attgaagtgc atttccaaaa catttttcct
    47941 ttccacaacc atatatatca ttattatacg tcattaacac agtgtgttca gaaccacaag
    48001 ctatagtctt aacatcatta atagaaacta aactaaataa atctctatta ttcttatctc
    48061 ctaaacctag ttgaccttga ttattaaaac cagtagaata gcatttattg tcattcttta
    48121 ttataaaagc tgatttatta tatgaaaata cagcctttac attgtcaaca tctacttttg
    48181 taaatgtatt tacatttgca gatgctccat tagttcctag acctaattga ccatacaaat
    48241 tatttccagc aacatataaa gttccatcat tgtataaaag atatgttgat tgaagtccac
    48301 atgcaatatc ctttacgcct tttatttctg gaaccttctc aaatgtagaa actgttttcc
    48361 ctactttttc taatcccaat cccatttgac catctgtatt tatacctgcg acaagtaatt
    48421 ctccatctga agtaagtata actgcatgag attctccaca tgcaactttt ttaatattgg
    48481 tagatatatt tggtattttc gtaaataatt ttcttgttgt attgtcttct cccaatccaa
    48541 gttgaaagtt actattatct ccacatccat acaagatatt attatttttt agaccaaatg
    48601 tatagttaca acatccagta aaaaaattaa taaagtcata attatcatac ccttcatcaa
    48661 attcatcaac taccatattt ttatcgccaa agaaattacc tctttgaagt agtttagttc
    48721 ttttcattat aaaaacctcc taattatctt atttcatacg aatatttatt taaagttata
    48781 tctgtctgac caattgaata tgccctatta gattttgttt caacaaagtt attatccatt
    48841 tcatgtatat atctaactct atttgcatta aatggtagca ccacattctt aaatgtaaca
    48901 ctaccaagtt ctccagttat acactttcta tttaaatcta acttaaggcc ttctatagct
    48961 attatgtttt tagcattagt taaggtatca taccaatatc cagcttcatc tacatctatt
    49021 tctgactcta attctaagta tattaagata tctaatactt tttcagataa tttattgtaa
    49081 ctatttttta atacagatag ttcagtttgt atttgacctc ctattacatc atcaatagat
    49141 atatttttaa ttttttctac tacctcattt atagctcctg ataagttttt agcttctgta
    49201 gataagagtt cttcgccaat ttttactttt atttctttta tatttttatc aaacttatca
    49261 tctaaatctt gaatatcctt ttgagttaca aaaaccaacg tcgggtctac ttctaatgta
    49321 acatttgaag tattggacaa ttgtaaaatc atttttacaa ccaaatcttt aatactgcca
    49381 tcttcagcac gtggcttata cgtctctgga tacttaccta tagctattat attattctca
    49441 ttatccagaa cagcagcttc tcttatcata aatccaccaa ctgttcctgg taaaatagtc
    49501 tctataacta tccaattagg atttttttca tcaattctta gagaatttat atttccttcc
    49561 caaactacat tctttaaagc agtttgttcc tctgttggct cataatattc tccattacta
    49621 tctccaactt gaaacttggt taatccaaca agctctccta gtgcagttgc atttgctatt
    49681 ttttctctac caattttggt taatattata taaaattttt cttccaatta taccacctcc
    49741 gatttaggat aaactactac attttctaca cctgtatcat ttgcaatagc tatattaatc
    49801 ttacaattat tttcaatgca tttaatgctt aaaggataaa cctttatttc ttctctactt
    49861 atcattccag ctccacagtt tacaacagca ctacatttta taattggttc aaagtatgga
    49921 tgaactttta cctcttcact acataccata cctccaccac aatagaccat acttttatct
    49981 agtattattg gttctaaata gtgtaataag tgagcaggtt ttactctttc aatcatatcg
    50041 ctacaatcta aaagagtttt atagtcacag ttatttgcta taaaactcaa tacgaatgta
    50101 aattcatcac tataaacttt tatatctgtt tctgattttg tatatgcctc acatatactt
    50161 tttataactt caatagtact agtacctctg cttctcattt tagcttttat attgctacgt
    50221 cttgtgtcaa aatcaagttc ttttttttca atgcagagta tgtcttccca catatctaat
    50281 ccccatgttg cagtgtcaac aaataattgg ttaaaggtat catcaatatc ttctttaaga
    50341 atatttaatt ctgtatcgta tgcaccttgt atctcctcta caatgtaatt tctatcaaat
    50401 gatggtagtt tatcaattag cttcattgat tttccacctc actaaaatta acagttgtta
    50461 cactaggtat tttatcttca ttaatagtta tattatctgt acttccattt ataagtaggt
    50521 tacttaaatc gtgtacacca gtagtattta taagtattcc cattactttt gtatagatta
    50581 tctctcctct aatatctcta aagtatgtat ttatactttc taggaaagat tcttttacat
    50641 tgtctaatgt atatccatct tctagtttca ttactgcact tatacttatt tctattggta
    50701 atggtgtcac aactgttata gttggtccaa taggcttctc ttcatctata tgttgctgac
    50761 acctttcaat tgtttctgta tcaacagctt gattattttt cccaaatatc aagaccttaa
    50821 ctgttcccgg accatcccat cttggataaa ccttaacatt atagactcca tctacttcca
    50881 aagcccattc ttcatagtga gctttatttc cacttgtagc ttgatttctt tggattttat
    50941 aaaatctttc ttttagttct tcatctgtct ctatatctgt accaccttca aaacttcttg
    51001 tgttagtaat ctttgttact ccagatatat tatcaactag tttaaattca caatttgtag
    51061 gtaaattata tttcttacca acttccagag cttgaactgg gctattgtca ccttcttcac
    51121 taccaatagt tacatctttt attactacaa atagtaaatc tctatatgat attattgtgc
    51181 cattatttat tacagtacct ttctctccaa taaattcaac ttctccattt gactctgtac
    51241 ctaactttct atatacacca aattcattga ctcttttatc aagaaactgg ttatatgtgt
    51301 cttgtataaa agccatttta tgcatctttg aaagttctag atatatcttc gaaagttcta
    51361 gattatttcc agataccatg ttgtttagaa aagaaccttc tcctttataa atatcaagat
    51421 taatattttc aagagttcta ttttttatta cttcatatgt ctggtcacta tacatttatt
    51481 tccacctccc catagatagt ggatactttt acatttgcac tcaatatgtc tctgttaaag
    51541 ttagcacttt ttacgtttac ttctaatata tatggattta ttagaagggc ctcttttatg
    51601 aatctactag cttcactttc tgtaagacct tttgtatatt tttgtcctat tagttctgaa
    51661 agctctgtcc catattctaa agagtatatc tcatgttcat atctatttgt ctttatacac
    51721 ttgtatatcc aaacttttaa ggcttctttt ttttcaatta ttttaaagtc acctttttct
    51781 aaaataggtt catctttttc aaaatcccat gccacttcac gaaagattgg caattcttct
    51841 gttttaggta agatataatc ctctgggaca cctataaaag gaaatattgt actcattata
    51901 agctcaccaa cttacttaca acagcaaatt tttcgcctat tctaaacatt attaccatat
    51961 ccccagattc aaagttatct ataaaaggat tttttacttc atgctggtgt tcttgatttg
    52021 tttctgtgtc aaatgtttct agctgtctat caatcatcca actatctatc aaaatatctt
    52081 ctttttctaa tatgatgtta tttgtctcta tttttaaatc tggtaattta cttttaattt
    52141 ttccaataaa aaaagaaggt tgattatgat actttccttc ttctcttatt attcctataa
    52201 attcatttat tggattagcc attatatcac catcctttta taatactctc ctagctgtgt
    52261 taaaatctct tctactactt aacttactta ttttaactac atcacctttt tgtggagcat
    52321 gtaaaaattc tccattacct atatataatc caacatggct gactggatta tgaaaaaata
    52381 ctaaatctcc tgcttgaaga tttttttgtt ctactttctt gcctttttta gattgttgat
    52441 ttgacgttct tggcaaacta acattaactt ttttaaaaca atagaccatt aaaccagaac
    52501 agtcaaaact acttggtcca tttccacccc atttataagg tttacctaga tgtttttttg
    52561 cttctgatat tactatttta gctttatcag atacattacc agaataacca ccaataataa
    52621 tttttccttt tcttcttcca aatttactag cttcttcaac atttccaaat aaaatatcta
    52681 tatggtatgt tccattcttt tctatagtta ttgcaggtcc tacgtcattt actttataga
    52741 ctccatcacg accagaaact cctgtaatta cctgtatttc atctccatag tttaataaag
    52801 gatgtttatt taaaaactct tttgtataat aagtttgctc atatttacca accataggag
    52861 cagcgcaagt ttttttagat gggtcaagtt ttttctttct acaatctgta tctccacctt
    52921 cttcttttct aggacagtaa gctgtaaatt ctgctgtaaa ctcttttcct cctgcataat
    52981 cttctccccc taaattactt tcttccttag gttcatcctg tcctgctgac ttttcatcca
    53041 taagattttg aaaattaagt tcaagctcaa tttgatattc tccattttgc caagtatgtt
    53101 tgtctgtatc tatataaaat agtcctacaa gctttgtata agaatcttta acttttactc
    53161 ctctaccagt tatacaactt acatctccat aacctttaag agaacagctt ttttctatcc
    53221 cattaaactc gctatcaata tctacatctt gattttcttg ttgctgaatt actttttgca
    53281 ttattacatt tacttcctta aaaatttcat tatctatttt ttcgctaatc ttgcttccat
    53341 actggtctac cactattact ttatttttta cattttccat gctctccgaa aaggtggtat
    53401 ttataatatt aaatccctct tcaaacataa cacttaatgt aacagttcct ttttcaataa
    53461 cattaaactt atctaaattg gcctctatca tatacttttt ctttgtcttt ttacttgctt
    53521 ctgtatatgc actcattatt gtgtcataac catttactcc tataaacatc tttgtatact
    53581 tgacattggt ctttgctatt attccaactg aaagcctatt ttcagcaaat acttgctttg
    53641 ctatgtcttc aactaactta tctttaaaat tgtatgacac ttcactttgt gtaagtaaaa
    53701 atcccatatc tttagataca aaactaattt cattactgct tgaatcttta gacctattaa
    53761 ttatcattcc tctaaagagt tctttctcat ctacatagaa acagactgta ctagctattg
    53821 gtatatcgat ttgttggaaa tttacatcaa atgatgattg tattattgaa aactctagtg
    53881 tccttgatgg agatttataa tcacctgacc atgatacctt gtctactatg tcagttatgt
    53941 catatatact tccgtttttt atgtgtaccc aaatcttaat aataaaaaat cacctgcctt
    54001 ttcatttaat tctatcagca gttatttata acttattatc tataatagtt attgttttgg
    54061 agttactttt ggtatcttta ttcttttata ctcttttaaa tctaatgtaa aatatacatc
    54121 tcgagtccca tctctttctt cataactaaa cccttcaatt atgacttcca tatttatatt
    54181 tgtttccgta attgtaaatc ttaatataaa accttccttc atccattttt ctatcttatt
    54241 tacacaatca tatggtgatg gaaaacctgt atagtcacag aaagtatagt ctttatttgg
    54301 aaaaaaactt gatattgaaa tgcttttaag agctacacca ccaaaagttg ctatttcacc
    54361 tattttgagt atactagaag agtttacagc agcttttcca ttaatactaa aggaagatgg
    54421 aaatactgga aatctaaatc tatcttcagc ttgtctaagc cacatttcca ttataagaca
    54481 ccccctaact gtgccaattg gatacttgca actaattttg atgttatctt ttctatatca
    54541 gcttcttctc taatcactat tgtatcagct aacttagcta tgtttatatt tccaccattt
    54601 tttctgcttc tatattgatt tgcttcttgt ttagttaaaa ccatttcacc ttcatggagt
    54661 ctggtttgat agtcattata aggaacatag ttcaatccaa atgcattatt acctttctta
    54721 cctgaataag ctttaccaga atcaccagcc tttgttccag catatggatt atctttgtaa
    54781 ttttttttac tcttttccac tagatttaaa actgttcctg tgatgcttcc attttttatc
    54841 ttattaatca tagtttgcca ccatttacat atatctttaa ctattttaca tactttatct
    54901 aatatgttaa aaaaagctcc caatattggc tctataattt tccatccagt ttccaacaga
    54961 ggtcctaagg tctcccatac agttttccaa ataacaccca gtgtctctat aaaattgttt
    55021 atttgaggtg catattcacc taaaaaagta aaaattcttg tgataatagg actaacagct
    55081 tcaactacag gtccgattag ttggaatgct gtagttacta ttgtaccaaa tatacttatt
    55141 aatccttgtg caattggtga tttattgttt atactatcta gtaaattatt tactaagttt
    55201 actactggaa gtaatgaatc tattaatgct gtaaaatcga tattttgtaa tagtgtactt
    55261 cctgcgttaa caattgcttt tggtgcattc cctatcattg ttgctgcatg attttcaaaa
    55321 cgttcttgct tattttcaac catcatttta cgcttttcag cctcattaga aattcgatat
    55381 tgagtagaat cagaaaactc ttttccattt tttacacttc tagattgcat ctggtcatat
    55441 ttgttgccaa taattatctt accattcatt acattgccta ttttaccagc cattttttca
    55501 gataagccag ctaaattgcc taatccactt tctataaaag tttttatttt ctttagtcct
    55561 tgacctaaaa tatcatcaaa aggtttaaca atactcttca agccattgtt aatgcttgtt
    55621 tgagctcctg atattatacc tggtattgta ttactcattt ctttagctaa ccccatctgc
    55681 tttacactta tgccttccaa tgcttcatca aaagttttat aattttctcc taccagagta
    55741 ttaagagatt ccatgtttcc ttttgctgca cttaagaaag ctgctgatac atcttgttca
    55801 ctacttgtat tcatatttaa agctctaaca tctaccatag cttgagttat atctttttct
    55861 tttttgccac cagtaatatt actaatcatt ttagctttag ttcccattgc aacaacatct
    55921 tcagggtcaa aaggcgtatc atttgccatt cttactaaac tgccataata atcatcaaca
    55981 tctatttgat taacttttcc agtatttctt ttcattacac tttctagaac aactttctga
    56041 ttttgttcct gaccaagtaa atcaacaccc tttttcacag cgacacctat agtagcacca
    56101 tttgtcaaat cagatatctt cccttttatc gaaccaagta ttccacttgc catatccttt
    56161 acagctattg caccactcca taccttacct gcaaataatc tcaatttggg aaatacttta
    56221 cttataatgc cacttgccat atcttttata gctattacac cactccaaaa agtattttta
    56281 aacaacatta acttaggcaa aattctaccc atagcactac tcaccatatc tttaagatta
    56341 atacttcctt gataaggacg tttctctact tgttttaatt ttcccaagat actacttatc
    56401 actctcttag tcatatcttt tgcttttatt acgatattcc aaatcttgcc accaatcttt
    56461 tttaaatttc caagtaattt atttttagtt ttttctactt catcactttt agctttaaat
    56521 attaatataa tcataatatt tgaattaaac tcatttttct tttgaatcaa cttattaaaa
    56581 taatcaataa tcatagctat agttattgct agtctcataa tacttccatt taaacttcca
    56641 ataactcctg aaacagcatt tatgctagaa cttaattttg tcatagttga acttacacta
    56701 ctagcagtag tttgaacttt tcttaacgag gaatttaaac tgtttactct attacttgca
    56761 cttccaatag aattttttat gctactagcc attacattag tggaaagtga aacagccaca
    56821 gttgctctcc taaaattatc agttactgat ataatttgat tcatttttgg tgtgtaatta
    56881 tctgtaaggt acatatttat accttcttct ctcacatttc ccattacata ccacctcctg
    56941 tcaaaggaca cactccatat ccatgtttca ttttttcaac ttcttcctca atttctaagg
    57001 cgaaaaaagc ttcaataagc tttaattcgc ctttatccat tgcataaaaa agggacggtc
    57061 ttataccttt tttcttccaa taatagtaca ttattgtagt cctggtatcc gtccctatta
    57121 gttttttacc tcttctatca ctgcattttt accataaccc ataacatctt gaagtattct
    57181 atataagttg gtaatttcac ctggtaataa taattttctt gctaattctt ttggtgttgg
    57241 agccttaaat ttatccatta gctccttatt tttaaataaa agctttccat ccaaatcaca
    57301 aacaccttct ataattgtaa gcatctgcat ctccggtaag tctatatccg ttttcatatt
    57361 ttcatcaatt ttaagacaag tattttgtat ttcttcatct ttacttattg ttaaagctct
    57421 acacattact ttaaattcct gcccaaatat agttgataat ctcttaagtt caactatagt
    57481 acttggtctt tctaagtttt ctgtatctga atttaaaagt aaatctacta tgtttatatt
    57541 ttttgataat ccattttcac tcattattat ttgctccttt atcaatcaaa ttcaatcaac
    57601 taactttttt acttattaac tatctgaatt tattaagtat atacttacta aatatcagta
    57661 taaaactaaa tataaaacta aattatatca agaaaatcat actcagtaaa tgtgaaagga
    57721 gcttctatct ctccttttac tccaacctcc caatcagcta aagttaaatc atcaaatgct
    57781 atatttttta ctgctattct ttctgctcca taagaatctg ggtcatttaa ttttgatata
    57841 gcaacaaatc taggttcaga acctctcttt atcttttctc caattaactt gtgcattctt
    57901 gaactaacgt ggtatagagt tattgagcct tttcctttat atcccatata ctttgtatca
    57961 gtacccattt gacctgctat tataatatct tcttttgtaa attccatctt tgcttgaaat
    58021 ttctttactt ctgctacttt gtttccatca agccaaagtt ctccccaagt accactcatt
    58081 acatttctag cttccatatt tgccatatta ataatcctcc tatatttcta ttgataaatc
    58141 tatatcttcc atagcatcaa gtacttttat ttttgctttt aaaaatactt tagaacctgt
    58201 gttagcttct tttatttctt gtaatgtcat ataagataaa tctactcctt ttgattttaa
    58261 atacgatttt tgtgcttcaa aatctatttc aacagtagaa tcagattcta taagtgctga
    58321 tttttctaat tcttctaaat aactttttat agctactatc aataaacatt tgttgtcata
    58381 actgttagta acctttccta tatagtcatc tattatcacc tttcttatgt cactatgtat
    58441 aatatctaaa gtgtcaacta tttttatttt ctggaacatt tctccttttt ctgctgttaa
    58501 ttcagttaaa gaatttactc ctctagcaat tcttatagct cctgcttctt taataagtat
    58561 aagctctcct ttattaaccc ttgattctgc atcaactttc gtcatcttag gtatatcgac
    58621 tacatcacta agtttagtat aagttactga ttgacttaaa ggtgtacctg ctataagtcc
    58681 agccaccctg cttgtaaact catcaacact gtatttcttt tctccaacta acacatcttc
    58741 tgtagtaaaa ttaattatcc cttcatgatt tcctacaact tttcctaata cagctttaac
    58801 cttaacctta tctatatctc taagtttaat tatccaattt tttatagcag tcttatcagc
    58861 ttctactgct tttggcatac atagataatt aaattcctta gtctctaaaa aatctaatgc
    58921 agtttgaata tctgcttctc cttctattac ataaactaat aatttatttg gagtgttaac
    58981 atttcccatc aaagctaaat ttatatattc tttattttca gcagataaat caactggtat
    59041 atcctctttt tcatgtattt cattaagacc tagtgcctta gcatctttaa gtaccattgc
    59101 aattattcct ctagctgaac gttctttaac agttgtagct agctccttaa atgatatgtt
    59161 gatacttggt aatcctatag ccatactatc actctcctaa atttaaattg atttctttca
    59221 tattttcata tactacatct tcttcatata cactatccag atagtttagt gtaaatgtaa
    59281 agttcaatac ataaattgac tcttcttttt taatactttg ttctagcttt gttatattta
    59341 attttctgtc tgttaccttt atatttctgt taaatagctt atttaattca tctgaaattt
    59401 catacattct aatactcttt gactgtggtt ttggtaagta ttttatagag ataacaatat
    59461 ctttgttatt cgtatttaac attacaggtg tacaaataac tgatagtata cttacaaaaa
    59521 aacaagaccc ttcagagcct tgcacacttt catcatcaat aaaaatactt tcagtaaagt
    59581 tttttttgag aatctcaata attgtttcta atatttcctt gtatttaagc aaataatcac
    59641 cacactatca ctaaatattc ttgcttaatc tgataatctt actataaatg atataagtca
    59701 tttttaaata tatatctcat ttattcctgt caagtatctt ctatattttt aggtaatatt
    59761 attcttttca atctatcacc tccttcaata tggatttctt tcacaataac attatctcac
    59821 atattttatt gcctattgtt gcaatggtgt atataaagtg ttgtaaaatt gtcctatttt
    59881 ttataatatc tcactcaatt taaatatcat ctttttcttt aaattgtagc aagatgttct
    59941 atctaagtgt agtttcatag aaatatatgt catattgttt tttgtcttac tattataaaa
    60001 aagattaaaa aattccattt ctatatcatt tagacacgtt agagcatttt ctatcttctc
    60061 tttttctatt tctatatctt ctttttccat ttttaatttt cttaatcttt cctctctttt
    60121 taacacctca ttttccacag aagagcttat gttatatgtg ttactagtct tttctgtata
    60181 taccattgct ccacatccag agtattcatt ctcacatctt tttatgtcca agttaatatt
    60241 ctttatcttt atatccaaat acttataatt atgtaatctt ttatctgttt ctttaaataa
    60301 tatatctttt ttgttttcca tagtacttcc ccttttttat atacttatta ttagcttcaa
    60361 atttacaata atctctttag tgcacagttt tttactaatc tcacaacttt caagaacata
    60421 ttgttctata actgtataaa aaaaacttct tctatatttt gttctaaaat actagaaatc
    60481 ttcttagcca taagaagtcc aggatttttt atacctcttt ctatttgaga tatataatca
    60541 tttgtaattc cagcctttct ggcaagttga atttgtgtca tacctttgca ttttctataa
    60601 tatttcaagt tatttttaaa cattgtttta tacctccatt actattaaga atattttgtt
    60661 cttcctcact tagattatat agaattattt gttctttatc aatagatttt agaaaaaaat
    60721 attcttttat attttattag ttttattttt tagaacaatt tgttataatc tatacataag
    60781 gtggtgttat aatgtttgcc aaaagattaa gagaacttag aaaggaattt ggattgactc
    60841 aaagagaact tggagaaaaa gtaggcgttt cccaaagagt cttagggtac tatgaaacag
    60901 aaaacagatt tcctgatgaa cacattttaa ataaattggc tgatgtattt aatgtatcag
    60961 ttgattacct tcttggaaga accttagtta aggaaaatat tgatacagta gctgcacata
    61021 gaaaaaatcc acatgaagag ttacctgaag aagcacaaga acaacttaat gattatatag
    61081 aatttttact aaataaatat aaaaaaaaat aaaccatatg atgagcagtc tatttttctg
    61141 ctcttttaat ataatttaaa ggcaaacata cattctattt tagggggatt tttatgaata
    61201 atttggataa gctttttgaa ttagcttctc aagaagagat aataattcat tataccactt
    61261 atattgcagg tgatttagaa ggactatata taaacaaaca tggtattaaa attatatcat
    61321 tactcagtaa tttaaaacaa aactcaaaaa aattgacatc catcttagct gaagaacttg
    61381 gacatcattt cactagcctt ggatactatg tatcctctta taacgattat tatacaaaaa
    61441 ttattataga taagtgcgaa aataaagcct taaaatgggc ttgtgagttc ttaattactg
    61501 aagaagatat aataaacata attaactctg gcatcacttg cgtttatgaa atggcagaca
    61561 tacttaatgt ggatattact ttctttcaaa aaagattaga atttctatca ttaaaaaaac
    61621 agtctttaca acttggaaat aataaatatt taatattaac taatttgccg tatttctaca
    61681 tatttgatcc aatttcataa taaaaatagt agtaatttta actggcttaa cagtaaaatc
    61741 tatatattta ttttaacaaa ttttggtttc tataaattca atattaatat ataataatga
    61801 tatcatatta tttaaaattc taaaaacaaa tatatgcttt tgttaagaaa ggttgtttga
    61861 tgaatgtata aagaaaagtt gtttgataat tattttaagt tcttagcact attattttgg
    61921 ccaattatgt ggtataagtg gatagtaatt tcaaatggta ctcttgaaaa tatgttattt
    61981 accatatatg caatcgtcgc a
//
LOCUS       DAEPIN010000015        60574 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.015, whole genome shotgun sequence.
ACCESSION   DAEPIN010000015 DAEPIN010000000
VERSION     DAEPIN010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 60574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60574
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.015"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            complement(<1..494)
                     /locus_tag="KR250_001121"
     CDS             complement(<1..494)
                     /locus_tag="KR250_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HBH0067085.1"
                     /translation="MEELKDFLERQLNKKINIYPYENQKLDASSHLVTFNNAIYVIDF
                     LDKNNLDNLKGNFYLIYQSHIDFEYLKNIFYNLFEDINIIQHNGFFIVNSKYNLDINV
                     TTQNIIETETYQSTYIFYLGELDSKADFDFRLQLCSDLLPHIIKDNAENKFLNLFDLI
                     RYKT"
     gene            complement(524..1633)
                     /gene="ugpC"
                     /locus_tag="KR250_001122"
     CDS             complement(524..1633)
                     /gene="ugpC"
                     /locus_tag="KR250_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003242648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HBH0067086.1"
                     /translation="MSEVILKNISKLYSNGFNAVKNINIDIKDKEFIVLVGPSGCGKS
                     TTLRMIAGLEEISEGELYIGDKLVNDIEPKDRDIAMVFQNYALYPHLSVYENMAFALK
                     LRKLPKDEIDKKVKEAAKILDLLPLLNKKPKTLSGGQRQRVALGRAIVRNPKVFLMDE
                     PLSNLDAKLRTAMRTEITKLHQQLGTTFIYVTHDQVEAMTMADRIVVMKDGVVQQIAT
                     PQDVYDYPANIFVAGFIGAPQMNFIDVILIEENDEIYAQNEHIKLKLNKEKHYDLIKD
                     NYINNEVVIGIRPEDIHVEDTFIKSNPDTCFKSRIEIKELMGAETYAHLKLGENTITI
                     RFDSKNRINVGDDLTLSVDNSRVHIFDKETTLAIR"
     gene            2067..2243
                     /locus_tag="KR250_001123"
     CDS             2067..2243
                     /locus_tag="KR250_001123"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0067087.1"
                     /translation="MNLKKKEKIVKPVLKKLTEIVLNILSKKILNYAIYAISVLIVTK
                     VVINIDLYISILYY"
     gene            2344..3081
                     /gene="cas6"
                     /locus_tag="KR250_001124"
     CDS             2344..3081
                     /gene="cas6"
                     /locus_tag="KR250_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HBH0067088.1"
                     /translation="MSRISIFMESCNGKFPNENSMLSVSFIKNILNAENKDFAKNIFN
                     YGEERKNNKQIKDINTAIYIPNLIRSKNELLVDGDIIFNISFYNYSMFSKLYNGILKV
                     KELEYKGYRFKIKNIKIHKEHEIKENGVIFKTMSPIIVKNKEGKYLDVEDSNYIECLN
                     YIANLTLESIRGSGLRKPLEFIPLNFKKRVLKEKIRGFKEREFYYINAYAGTFFLKGN
                     MEDLNALYKMGIGYRRTENAGMVDILK"
     gene            3086..4561
                     /locus_tag="KR250_001125"
     CDS             3086..4561
                     /locus_tag="KR250_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0067089.1"
                     /translation="MREIKAKFYRSGDIVRDIGIVYFCELLMDLKNELEKNDYKLKFT
                     CELNRNYLSLESAEYIDPKYISDYILENQLFKVFKNGKKETLEKIFSNIDNLTYVNFI
                     EELDKSSATEKQKEGIKKDFKNRRFPYVRNSGKYGLNTSLENMEKNVKRIVDIVIKSN
                     IEIDEIDKLDLKQYEEKDSVCNVCNINKTTKLDIDLRERKDSKYNFLFRGAEKSGFKR
                     SGQVESNICFECEFFNLMCLLYINLKRPMVFAYTNDLRELAFLNHKIMLKRKMYSDKS
                     FYKKLLHEKISSIRLYRIDIDTNKGIILKFDSIIEYKELLKKIELIDIIDNYNFSREP
                     MNTRNLGKKMINNGNLSNLKELLLDNISVLRQLTGTSREMDFVSSSYNIGLYIKLCWI
                     VDGREEYKMKNYIESNLIYSRVGKDLFNKMTDESRRKNFSMKVIQLLKSNDRTQLFQT
                     IMHVLVSNGILIGEGFVEGLMQSSELELNTNVGLFIQEIMK"
     gene            4581..5597
                     /gene="cas7i"
                     /locus_tag="KR250_001126"
     CDS             4581..5597
                     /gene="cas7i"
                     /locus_tag="KR250_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein
                     Cas7/Cst2/DevR"
                     /protein_id="HBH0067090.1"
                     /translation="MNEKRVKGVTLTILTESPVPLSYDQGYGNYTPIKKEQYRDKVHA
                     KTSIATITYDLRRILHQEYGWNLSNIVFGKKGNIYPSIKKNVGCVDENGLETDVFGYL
                     IPLKEKGSISKASPLRIIPFRSLNSYRGSTQLITNRGFLSSEFGRKYYDEREENEVLR
                     DENFPTTQALAIEETLSDYYVYTITLELDRIGVVEVEEDGKLLLPEERKFMSKELREK
                     AVKDILDAITVFTRNIKHSSVLLKPLAVMGGAFDKVVPFFWDDVDYNADSGEINLEGV
                     IETIESYSLKESNTILAINDRLKISNKKELNKFNLSKYPVKEIKNLADRLEIGEDNMW
                     YLKE"
     gene            5613..6410
                     /gene="cas5"
                     /locus_tag="KR250_001127"
     CDS             5613..6410
                     /gene="cas5"
                     /locus_tag="KR250_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas5"
                     /protein_id="HBH0067091.1"
                     /translation="MKVLQCKLKQPTAHYRDPKVFQNEYISTLNLPSKTTIMGMITYL
                     CDRRLNSDIDIGIIGTHHHRELEFSRGENIDFWNEYTNMRKGKDKEKFLLQGNYYDYY
                     KEHKAQNSILNYEVLKEVELTIFISCKDDEELEFIRKKLESPCKYANLGRKEDFVIPS
                     EKGCFVKEVELKEVIPMNTRDAIKENIKLKNTYVRVDLRDKENVETIINQGVLIALPY
                     KYKDLEANRDDRVYEFCHYIYVDNDGIYPKNIKVNVCIDTKEVFTWL"
     gene            6437..8845
                     /locus_tag="KR250_001128"
     CDS             6437..8845
                     /locus_tag="KR250_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HBH0067092.1"
                     /translation="MKNIMQKEILAKSYNLRTNQQEQTLVEHIKDLFEVLESILELNL
                     YSDKDVEILKICCALHDLGKINSIMQQKMEINNKISYSCSEEERKKLESDKKSLKKIA
                     RHNIFSGAFLKDILEKMNLSEEDKLYIYKSIMLHHGNYEDYMRLSISKVQEEIYEYIE
                     KGILEKEEFNLKDIESYINSILNVDFKFGEDVLDYDYIDKLSESMFIDSDYNNGQIDD
                     SVLNYRKFKYILYKGMLNLIDHSASSRQKGIKFYNDFTDEEIDNMILNEIYKSQGNLE
                     KNNIEFNTIQKRLRELSGRNVLTVAFTGSGKTAADYRKTFKRKFFLVPNKISAESFYR
                     KNIFQNKNDLDTRSSNDYIGLLHGDINLYSENEDNGEHDFVLTLRDIDLSINFCKPCV
                     IATVDQLLLSMFKFPGYEKIFAAVKNASITVDEVHLLEPKMFLILIYFMQFACKYLDV
                     DFHLMTATLPKSYKEQMINKGIIFQEESNENVTDKGEIVFIESNKEEEICEGKDVKVS
                     FIKEKEIKSIVEDALENKQKILIIKNTIDSVNRTYEFLKENLSDKYGGVDIDVLHSRF
                     KFKDKKEKYSKILNGEGDIWISTQSVEISLDLDFNIIISDLATMDSLIQRMGRCNRNN
                     KYEYGNFYILPSEDKIYDNKLKNTTKNILKDILKTNSIFTMGIRKTILDDYYDNSVVK
                     KYFEENFISCDKEIKNIYGINKEIFDGLDLIFNFEPYKNIVDSKKEAAKIFRDVDVSY
                     KIILEEDFYKEDRELQQDSIQVSGFIFNRLYYYGLISKVEGYMVLKSSSKFEYNSTVG
                     LILK"
     gene            9287..10234
                     /gene="prmA"
                     /locus_tag="KR250_001129"
     CDS             9287..10234
                     /gene="prmA"
                     /locus_tag="KR250_001129"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HBH0067093.1"
                     /translation="MNNWIEVTIKTTTEAVEPITNILYEQGAGGAVIEDPKDFLFQKK
                     NELDWDYVEEEVFKKNEEDDVLIKTYVSEEKNVMEFVEIIKQKVLGLKDFGIDIGEGS
                     VSLDQVNEADWANAWKAYYKPTKVGQRVVVKPTWEDYAMQEGDLIIELDPGMAFGTGT
                     HETTSMCIRELEKYVNKDSKVFDIGCGSGILAIAAAKLGAKEVVAVDLDEVAVKVAKE
                     NVLENKVEKSVSVMHGNLTDVIKDKADVIVANIIADIIKILAKDVQNFMKEDAIFISS
                     GIILDKVEEVKESLIENGFEIVEVQKLGEWSAIVSKLKK"
     gene            10253..11011
                     /locus_tag="KR250_001130"
     CDS             10253..11011
                     /locus_tag="KR250_001130"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HBH0067094.1"
                     /translation="MDRFFVEKNNINLQDKTCTIEGEDVKHISKVLRCKLGEKLEICD
                     KNNNEYICEIMNIDKSIVNLEILEKVDINRESELKVRLYQGLPKAPKMEMILQKLTEV
                     GVEEIILVQTKRSVVKVDDKKEDKKFERWERIIYEAAKQSKRGKIPKLRGVLSFKEAL
                     EDMKKNNVNICPYENERTVSIKHALKKCDSNIDSVGIFIGPEGGFSEEEIEQIQKNNC
                     NVVSLGPRILRTETASVVASTIALYELSDLGGEK"
     gene            11013..12311
                     /gene="mtaB"
                     /locus_tag="KR250_001131"
     CDS             11013..12311
                     /gene="mtaB"
                     /locus_tag="KR250_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HBH0067095.1"
                     /translation="MKKVAFYTLGCKVNQYETEAMLELFEKDGYEQVNSEEYADVYVI
                     NTCTVTHMSDRKSRQYIRRVKKKNPDAIIAVVGCYSQVSPEEILDIEEVNLVMGTNDR
                     RKIVEEIKKINSSKKVSTVDDIMKVKAFEEIEISQTNGKTRAFMKIQDGCDRFCTYCI
                     IPYARGRVRSRDIDSIVDEVKKLANNGYKEVVLTGIHVASYGKDLKDRDIKLLDVIKQ
                     INQIEKIERIRLSSVEPILFTDEFVNEVLKMDKVCPHYHLSLQSGCDETLKRMNRRYT
                     TLEYKTIVDRLRSKMPDVAITTDVIVGFPGETNEEFKKTYEFLKEIELSQMHIFKYSP
                     RKGTPAATMENQVDPQMKHFRSEQLLNLSKVNFNKFATKFIGRELDVLFEQNIEGNKY
                     EGLTSNYIRVVVESDKNIQGQILKVKINDVKDEYVEGILL"
     gene            12354..12704
                     /locus_tag="KR250_001132"
     CDS             12354..12704
                     /locus_tag="KR250_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HBH0067096.1"
                     /translation="MDCIFCKIANGEIPSTKVYEDDRVLAFNDLNPVAPYHILVVPKK
                     HYDSLIDIPDKEMDIVSHIHVVINKIAKEKGFDQTGFRVINNCGSDGGQEVKHLHYHI
                     LAGKKLPNYEAGQN"
     gene            12867..13046
                     /locus_tag="KR250_001133"
     CDS             12867..13046
                     /locus_tag="KR250_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HBH0067097.1"
                     /translation="MSEVRVRENETLDSALRRFKRQCAMSGIMSEVRKREHYDKPSVK
                     RKKKAEAARRKNAKK"
     gene            13076..13519
                     /locus_tag="KR250_001134"
     CDS             13076..13519
                     /locus_tag="KR250_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HBH0067098.1"
                     /translation="MSLKQKLQEDLKSSMKNKDTVRKSVVTLIRASIKQYEVDNRVEL
                     DEDGIIDVIAKQLKQRRDALVEFEKAGREDLIKETEGEIEVLKEYLPQQLSEEELEEI
                     VKSTISEVGATSMKDMGKIMSVIQPKVKGRADGKLINKLVKQNLQ"
     gene            13630..14127
                     /locus_tag="KR250_001135"
     CDS             13630..14127
                     /locus_tag="KR250_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HBH0067099.1"
                     /translation="MIISVKEIRNFLINIFIPLIIGYLSSILTMIISGTDISTYYLQL
                     TKPSFAPPAFIFPIVWTILYILMGISSYLILRKGYNLPKVRDAIFYYGLQLVLNFIWS
                     ILFFGFGLRFTALIDIIIMILVSIIMISKFSKIDKRSGYINLIYLMWLVYAGFLNYFI
                     WFINK"
     gene            14320..14883
                     /gene="raiA"
                     /locus_tag="KR250_001136"
     CDS             14320..14883
                     /gene="raiA"
                     /locus_tag="KR250_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HBH0067100.1"
                     /translation="MQIIVSGRQMKLTDGIKGYVDGKLSRLEKYLDPESEVKVTVSAK
                     KDRQKVEVTIIPINGQIIRAEDVEDDLYAAIDIVCDKLSRQVVKYKTKVKDKVQNNKS
                     IRFENLDFIDNSSEFDDYDYDDEEDENIVIERRKKFNVKPMSSEEAILQMELVGHNFY
                     MFRNQDNFEINIVYKRKAGGYGLIEQD"
     gene            15096..15332
                     /locus_tag="KR250_001137"
     CDS             15096..15332
                     /locus_tag="KR250_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YabP/YqfC family sporulation protein"
                     /protein_id="HBH0067101.1"
                     /translation="MLEISTDLQVNQPVITVTSNTFISIENYLSILEYEVDLIRIKTK
                     VKTIKISGDKLSLKYITDSEIGIKGIIYNVEYVD"
     gene            15345..16688
                     /gene="yqfD"
                     /locus_tag="KR250_001138"
     CDS             15345..16688
                     /gene="yqfD"
                     /locus_tag="KR250_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YqfD"
                     /protein_id="HBH0067102.1"
                     /translation="MISFIRGYYVIVVEGVGLEQFLNHLIRNGISVYNVTRIKNTKME
                     FHIDRQDIKEFKNVYRGSKFDIKVKQKTGVPFIIKRVYKHKGMWICALVSLFLLMSTS
                     QFVTDVYIQSPEGIKKEALRNELYKVGVRPGVYKKSIDRKEVRDHMMSKFNDVAYLSI
                     NVKGTNIFVTVTKKAESLKSTDQSNYCNVIALKNGIIEKVIPRSGKSVIKSGDIVQKG
                     DVLLNGANTKSIPEVWASTFYESTKKASYVDTVNKKTGEKKNIYTLSFYDKEFTMRKN
                     IKYKDYVVENKEKKLSIGNYTFPIKIKTSTFYETKKVEVKRNKEELKKELSEKALKEL
                     EYIIPASARIIDVKHNFKVNKNMLEYLITVQTSENIAKIYPLSKSEAERFIKEESKPD
                     EGEEEVPSNPEKRPLNDIRNEFDEDNKDNKDQNNSDENNSNQNSNNSQNNNSNNN"
     gene            16627..17742
                     /locus_tag="KR250_001139"
     CDS             16627..17742
                     /locus_tag="KR250_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HBH0067103.1"
                     /translation="MKIIVIKIVIIVKIIIAITTNKIKLKILGGLIQLIIQKKFTIAD
                     GNFERELFGNFDENVKLIEKTLNIDVILREGNIILIGEEKNVDSALKLMNELHQTVSN
                     GKHLDKQSISYSLSLLLEGSEQKIKELEGTIVITQRGKAVQPKTLGQKEYIKLIENND
                     ITFGVGPAGTGKTYLAVAMAVKAFKRDEVSRIILTRPAVEAGESLGFLPGDLKDKVDP
                     YLRPLYDALFEMLGADKFNKYLERGTIEVAPLAFMRGRTLDNSFIILDEAQNTTSEQM
                     KMFLTRLGFGSKAVVTGDVTQTDLPQNKKSGLIQATEILKGVPGIGSIMLTDRDVVRH
                     ELVQRIIRAYEKHDKREEFKKEERKKAKMQEKTFKRK"
     gene            17799..18260
                     /gene="ybeY"
                     /locus_tag="KR250_001140"
     CDS             17799..18260
                     /gene="ybeY"
                     /locus_tag="KR250_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HBH0067104.1"
                     /translation="MDLILDDRQDKLEVSEELIEKIKDIIIECLDYEGYDDNYEVSLS
                     FVDNKEIHELNREYRGVDRVTDVLSFPLLSDDFEDVELEEESLGDIVVSLERALEQSI
                     EYNHSFEREVCFLICHSMFHLLGYDHDTDENTKEMREKEEHILNKLNITRE"
     gene            18266..18985
                     /locus_tag="KR250_001141"
     CDS             18266..18985
                     /locus_tag="KR250_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HBH0067105.1"
                     /translation="MKPEKTRQGIIKAFNAAIEGILYTFKFERNMKIHYLGSVAVLII
                     SLFFNFSKLEMIMLLMSICLVVVAEMFNTAIEKAVDLVTDEYHVLAKIAKDVAAGGVL
                     VAALNSVVVGYILFYDKLTDISGILIYKIRESELHITLICILLVLIAVVVVKALTSTG
                     TPLKGGMPSGHAALAFAIATAITLMTERVVASTLAYIMAVLVAQSRIEGKIHTFWETI
                     AGALLGVLIAILVFQLGMFYN"
     gene            19104..19505
                     /locus_tag="KR250_001142"
     CDS             19104..19505
                     /locus_tag="KR250_001142"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HBH0067106.1"
                     /translation="MDNIELLRLAEDARQHSYAPYSGFRVGAALLTKSGKVYTGCNIE
                     CASLGGTNCAERTAIFKAISEGDKDIYKIAIASDNSENNEQTYPCGICRQVIIEFGSD
                     IKIITGYTKGEVFEHSIKDLLPNYFSGNDLK"
     gene            19569..20462
                     /gene="era"
                     /locus_tag="KR250_001143"
     CDS             19569..20462
                     /gene="era"
                     /locus_tag="KR250_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HBH0067107.1"
                     /translation="MFKSGFVSIVGRPNVGKSTLMNNVVGEKIAIMSDKPQTTRNTIQ
                     AVYTDEEMQIVFLDTPGIHKPKNKLGEFMVKAATEAFKNVDLILFVVDDSKKIGPGDR
                     KIIEDLRSVKTPIILVVNKIDQLDQKDELFDIIKMYDREGIFKEIVPISALKGKNTDT
                     LIKVIQNYLEEGPKYFPDYMITDQPERVLIAELIREKVLHYLNDEIPHGVAVEIEKMK
                     ARNDKEIVDVSAVIYCERDSHKGIIIGKNGRKLKGIGKSARQDIELLLGSQINLQLWV
                     KVKENWRNLQNYINNFGYNDK"
     gene            20474..21853
                     /gene="mgtE"
                     /locus_tag="KR250_001144"
     CDS             20474..21853
                     /gene="mgtE"
                     /locus_tag="KR250_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HBH0067108.1"
                     /translation="MDRKLDASQDLLYEVKSLIDNNKVLELRELIEEYHIIDIFDIME
                     NLEEDMKIQLFEVLPLDMASSILEEGSVEFFISILSKLDVEHSKNILELMSLGDMADK
                     LSELEEEEREHIINLLNQENADYVKELLFYDEDSAGGTMTTGYISINKDMTALEAIDH
                     MREEAEEAETIYYIYVVDDEEKLVGVLSLRELIIARDANIVEDLMSENIISVYVDEDR
                     EEAVRLVSKYNLIAIPVVDRQEKLKGIITVDDIIDVMEEEATEDMYKFAGSSEHEREV
                     AEKENPTLREQIISALRGRLPWLIITLVGGLLASLILSNLDYIMNPVYAPLVFFIPVV
                     IGMGGNIGTQSSSVTVITLSNKDLNFSNVVREGIVGIITGLLCSIITGIVIYFVMRKL
                     DIVLIVSISLFINMVLGATIGAFMPVLLKKMDADPSTVSSPIISTALDITGIAVYFII
                     TTALLSKIV"
     gene            21926..22081
                     /locus_tag="KR250_001145"
     CDS             21926..22081
                     /locus_tag="KR250_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqzL family protein"
                     /protein_id="HBH0067109.1"
                     /translation="MNNISWEVFKKTGSIDAYLYFCDCKNIHEEIEEVREKKEDDDNP
                     EHPGDST"
     gene            22047..22820
                     /gene="recO"
                     /locus_tag="KR250_001146"
     CDS             22047..22820
                     /gene="recO"
                     /locus_tag="KR250_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HBH0067110.1"
                     /translation="MIILNTQGIVLKAIRYKESDIILTLFTRKLGKVSAIAKGAKKNK
                     SSLLSSSQLFSYSNFTLKKQGNMYKVTQSEIIKSFYNISYDIEAFSYATYITKLVENS
                     ILENQTNNRLFILLAQTLYLYTQDNTDNRFITAAFELKFLDYIGFKPIVNKCINCECK
                     ILQNSVFNIYEGGILCSKCSKLFDNNIKLDLTTISLMEYVLRNDILTCSKAKVSKYIT
                     HELENILDKYLKVYVDNINFKSLHVLQSVKNNKGVDNDE"
     gene            22813..23412
                     /locus_tag="KR250_001147"
     CDS             22813..23412
                     /locus_tag="KR250_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4342 domain-containing protein"
                     /protein_id="HBH0067111.1"
                     /translation="MSSNITIEMVDSVFERVPGATYAEAKEALVMCNGDVIEAVIYLE
                     NKKNTCNCKAKSAKETMEEVFGKDGENIKFQLKELLRKSSVVRIIVEKDGKTMMNIPL
                     TVGVVGLAFVPLATLVGLSAAVISKYRIKVQNEEDGEIVDLGELNEEKLHVLKNMITN
                     AAKDVKDVVVDKKEDDKDVTADLMKEESEKNKEDEDENK"
     gene            23572..24450
                     /gene="glyQ"
                     /locus_tag="KR250_001148"
     CDS             23572..24450
                     /gene="glyQ"
                     /locus_tag="KR250_001148"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HBH0067112.1"
                     /translation="MNFQEMILALQKYWSKQGCIMMQPYDIEKGAGTMNPNTFLRSLG
                     PEPWQVCYVEPSRRPADGRYGENPNRLYQHHQFQVILKPSPDNIQELYLESLKEIGID
                     PSEHDIRFVEDNWEAATVGAWGLGWEVWLDGMEITQFTYFQQVGNIECELETGEITYG
                     LERLAMYIQEVDSVYDLKWNDKITYGEVFNKAEYENSMYAFELCDADMLFNLFDIYEK
                     EALRLMENGLVIPSYDYVLKCSHAFNTLDARGAIGVSQRASFIGRVRNMAKTVAETFV
                     KQREEMGFPLLKDGDK"
     gene            24450..26516
                     /locus_tag="KR250_001149"
     CDS             24450..26516
                     /locus_tag="KR250_001149"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HBH0067113.1"
                     /translation="MKNYLLFEIGVEEMPSRFVGSTLEQLKNNLSKLFDENRIAFDKI
                     NAYGTPRRLTLVVEGLSDRQSDLEEEIKGPAKKIAVDAENNLTKPALGFIKSKGLSES
                     DIYFKQVGKEEYIFATIKQDGKETSEVLKDILPETIKSVVFPKAMRWGGKNLKFARPI
                     RWIVALLNDKVLEFDLEGIISSNVTKGHRFLGESTIEVDSIEDYFEKLEKNYIVLDQN
                     KRKEMIRKQCIEVANSLGGEVEFDEDLLEEVTYLVEYPTAFYGEFDVDYIKLPKEVVI
                     TPMKQHQRYFPVLKEGKLLPNFIAVRNGDSYRIDNVKAGNEKVLEARLADALFFYRED
                     TKRNLESYIEKLKTVVFQVKLGTIYDKTLRLEKLSADILDSLGLVDEKADTIRAAKLS
                     KADLVTGMVGEFDELQGFMGKEYAKVGGENDVVSEAIFEHYLPRFAGDILPKTNAGVA
                     LSIADKLDSIAGFFAIGIQPTGSQDPYALRRQALGILSILMDRKVAVDIKILIEHALD
                     NYSELDFNKEEVVEQIMNFFNERVKNLFKDLGIRYDVVDAVLSSDINDVSDMHMRALE
                     LNNWLEKDELVEMLTAFNRVSTLAQKAESDKIDESLLKEDAEIKLYNEFKQVKVKVNE
                     LLKDKKYGIALDEFASLRPVVDNLFDTVMVMDNDEAIKNNRLALLKQVYDTMLGICDL
                     SKIVYK"
     gene            complement(26682..28229)
                     /locus_tag="KR250_001150"
     CDS             complement(26682..28229)
                     /locus_tag="KR250_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HBH0067114.1"
                     /translation="MDNFKKILLNELPNFKEYSSKFLNGEINKMQYKGFSGGYGVYAQ
                     RDKKSFMIRLRVSAGVLSQYQLNKIYEIAIKHNLDKIHLTTRQAIQLHDLSINSIVDI
                     MEEGIKNDIFTRGGGGNYPRNVGLSPLSGVDPSEVFDVTPYAVATDKYFIKNATTYHL
                     PRKLKVSYSNCYTDTAHCSIQDLGFVATLKNDEPYFRVFLGGGLGKQPKVALELDELI
                     KPKDALYCVEGMIKFFMDYGNYENKNRARVRYMVESLGEELFLEKFKEYYKLEKENGS
                     LELNIEEIDYSKPGVKIDIQDSRLIPQKQDGLYTVYIHPVGGILLTKDLSTLLKELDN
                     VENPMIRLGMTEGLYILNLNGNEAKRILEISKTISCNSQLEQSISCIGVPICQMGIQN
                     SQKMLHEIIDYFRLQNNEEILNKAPKLYISGCLNSCGVHQVGSIGLCGKKKNVDGIST
                     DAFELFVGGSFEIGKTRLGKSLGDFKASDIPEMLYKISDASSGNFYEWVNSNDNILNK
                     ITDKYKI"
     gene            28509..29276
                     /locus_tag="KR250_001151"
     CDS             28509..29276
                     /locus_tag="KR250_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HBH0067115.1"
                     /translation="MGEIINALDRALDIILLLYHEKREMGITEISKAMGVYKSTVHRT
                     LVTLENKGFVIQNAENGKYWLGINLYAIGMVVGEKMSLTEIVKPYTKKLNQEFNEVVN
                     VSILEERAQDSPRSIIIHKEYGSNQLLSVNPSVGSSSECYCSAVGKCLMAFNDSIDFE
                     KYRKTPIHKYTEHTIDNWDDMMLFLAKIKEQGYAIDDEELEHGLTCIGAPILDKNNKA
                     IAAISLSGPTIRMREGDFEYKIKRVIETAKSISELFR"
     gene            29559..29756
                     /locus_tag="KR250_001152"
     CDS             29559..29756
                     /locus_tag="KR250_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBH0067116.1"
                     /translation="MEKVKLSVERCKGCYLCIEACKKDAISLSDYTNKKGFVTIKVDE
                     EKCIQCGACYTMCPDLVFEIL"
     gene            29785..30861
                     /gene="vorB"
                     /locus_tag="KR250_001153"
     CDS             29785..30861
                     /gene="vorB"
                     /locus_tag="KR250_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HBH0067117.1"
                     /translation="MQSLLIKGNEALAEGAIRGGCRFYAGYPITPQSEILEMMAWRQK
                     EVGGVFIQGESEIASVNMVMGASALGARAMTSSSGPGFSLKQEGIAYWVSAGLPAVVA
                     CVQRYGIGDGFIGAGQDNYWQAVKGGGNGDYHLIVLAPNSVQENMDMAYESFELAEKY
                     MHPVLILSDATIGQMVEPCIAPPMKEYDMDKAPWAAKGTPVGEKNKVITDITYFEDQD
                     TWQKGYIEKMRKVQEDEQRWEEIEIEDAEVVFVAYGISSRIAEGAVRDARAKGMKLGL
                     IRPKTLWPYPRKAFKKLSDSLKGLVTVEMNMMAQMKEDVIIACDNRFPVYSVATAQYM
                     AEVNQLIDFAQQIIDGKAKQEEVF"
     gene            30864..31583
                     /locus_tag="KR250_001154"
     CDS             30864..31583
                     /locus_tag="KR250_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HBH0067118.1"
                     /translation="MAKKAPAIMMCENMFCGGCGHSIVDRIIGEVLTEMDICQDTIIC
                     SDIGCNHMFQFSMNVDVVVPPHGKMGAAMAAMKRVRPDKYIFTIAGDGGAYAIGLGET
                     MSAATRNDNVIFLVMNNNMFAMTGGQMAPTTIEGQYTASTPAGRNYADHGSTFDVVKV
                     MGNLDIAYLARGTITNPKEINTTKRYIKKALEKQKEKKGFCLIEVLVPCPTNWGLKPV
                     DAMKRIENELVPKYPLGEFID"
     gene            31596..32135
                     /locus_tag="KR250_001155"
     CDS             31596..32135
                     /locus_tag="KR250_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HBH0067119.1"
                     /translation="MLNEIICAGFGGQGVLTAGKIIMYVAYKAEKKITWFPSYGNEMR
                     GGSANCNVVVSDEKIANPCVKNPDILLGMNSVSVDKYESLMKKNGYMFVNESLVSKDR
                     KYREDINVIKVPVTEIAQNLGNEKAANICMLGAMVKSLDLFTKEEFESGMCEYFENQG
                     KGKFNSKNVEAFEAGYDYE"
     gene            32292..33350
                     /gene="buk"
                     /locus_tag="KR250_001156"
     CDS             32292..33350
                     /gene="buk"
                     /locus_tag="KR250_001156"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HBH0067120.1"
                     /translation="MKVLVINPGGTSTKISVFQDENEILKKNITHTREDLKNFSKVFD
                     EYDYRKQLIVDILSSENHSINSFDAVVGRGGLMKAIKGGTYTVSEEMIEDMRNEINGE
                     HASNLGALLAKTIADEIGVQSFVVDPVSVDEFDDVSRITGISDIEKASWLHALNHKAV
                     SRKIAQKLGGKYEDYNFIVAHLGSGISIVAHKQGKMIDGSGGRSDGPFSPERSGGLLA
                     YPLIELCYSGKYTHDEMVAKVSSIGGMYDYLGTKDMIEIENRIKEGDEKAKLIMDAFI
                     YQVAKEISMYGASLSGNVDRVILTGGISHSEMVVKGVAEKVSYLAPIEIVPGEMEMEA
                     LALGALRVLNGEEIAKTY"
     gene            33368..34261
                     /locus_tag="KR250_001157"
     CDS             33368..34261
                     /locus_tag="KR250_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HBH0067121.1"
                     /translation="MLKDFNNLLEEAKKLEPVIVSVAVAEDKEVLKAVKSATDLNIIK
                     PILVGDKDKIESISREIGLKGHEIIGCQNPDESVKKAVEIVKNKEAKVLMKGLVNTSV
                     YMRAILNKETGLRTGRLLSLLAVYELPQYHKLLYCTDSGINVSPNLNQKKDIMTNALL
                     TMKSIGMENPKTAILTANEMVDPKIQSTVDANLLVEMEKSGEIPKCIVEGPIAFDVAF
                     DAHAAEHKGIKSNIAGDVDLLVFPNIEAGNILGKSWLRFNKAKWAGIVLGATNPIILG
                     SRSDTAEIKINSIALACLASQ"
     gene            34291..35499
                     /locus_tag="KR250_001158"
     CDS             34291..35499
                     /locus_tag="KR250_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBH0067122.1"
                     /translation="MKYAKRMDMVKASAIRDSQKKIAQKVASGGTVISFAAGLPDPNL
                     FPIEEISEITQKVLNERGKFALAYGPTKGEDELLDLLVKRMKEEENIDTKAENIIITT
                     GSQQGIALSAMILLEKGDVVVTENPSYLGAINAFRPYECDFLGVDTDEEGMVTDDLDK
                     LLSENSNIKVIYVIPTFQNPTGKTWSLQRRKDFMEVVNKYDVIVIEDNPYGEIRFTPE
                     PLPTLKSLDTKDKVLYLGSFSKVLCPGFRVAWMCGNVDIIDKAELLKQGIDLQSNQFS
                     QVQVIEFLKKYNLNEHIEKIRAEYKKRCLLMLDAMKKHFPEEAKYTEPDGGMFIWVEL
                     PESINVDDLLDKAIDAGVAYVSGESFFANNGPKNTMRLNYTTMSEEQIVKGVEILAEV
                     IKKELNYACE"
     gene            35523..36782
                     /locus_tag="KR250_001159"
     CDS             35523..36782
                     /locus_tag="KR250_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBH0067123.1"
                     /translation="MGEKENGIKFGKLMFQLVIIAISWELVYIIPFIQYTLYDPILKA
                     LECTNTQLGFLLTIYGLGNIFGAPIGGWLADRFDYRKIILGSVFLNGVVSFLFAFNMN
                     YPFAVATWVGCAITSLVMNYPSMVKILRVIGKDNQGKVYGFNEAMVGVSGVVMGAIFL
                     YIYTCFATPTLGMRWVMISLGILSIAMCPVLWFVIKDVDIKEEREEAGEKMSAGDFIT
                     VLKSPNTWLVGISIFCVYSFTVTMSYFTPYITSVLGGSVALSGALAIIRQHGLKLFGA
                     PFGGYCADKLKSPTKVLLPIYVFGIAVIVLFLVLPASTPMTIFIALTFVVGILGYMGK
                     GIYYAVQDEVKVPVKYSATTIGIAAALGFSPDVFQFALIGHWIDTYGNKGYTYTFIFQ
                     IAILVIGILSCLYILKVKKRRASKLEA"
     gene            37427..38890
                     /locus_tag="KR250_001160"
     CDS             37427..38890
                     /locus_tag="KR250_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HBH0067124.1"
                     /translation="MTDLVNTINGIVWSPVLVAMCLCIGLYFSLKLKFFQIRDVKEMF
                     TLLLEGDSSHVGISSFQGFATSMAGRIGTGNIAGVAVAIAMGGPGALVWMCIMATLGS
                     ATAFVESTLAQIYKDEHDGQYRGGPPYYFEKGLGWKKCSVLFSIVAIISYTFFMPGTQ
                     SNTLYLAMNQAFGFSKMTIAVASSIALAIIIFGGIKRIGLFAEKVVPIMGGAYLIMTL
                     VIIITNISLVPEAIVTMFKSAFGFGSVFGGMLGSAISWGVKRGIYSNEAGEGSGTYGA
                     AAAEVSHPAKQGLVQAFSVYVDTLLICMATGIMIVITGMYNVTGVCTALPGVEPGPQY
                     VQSAINTLHPQLGDIFIAIVLLLFTFTTLLAFSYMMETNVSYLVTRIKAEKHEKYIIN
                     VCRFMLIACAAMCCFIEPLTSWALGDICIGVLTYINLIAIFTLKKPAIKAYYDYIRQK
                     KMGIKRSDRTFNPIELDIRNAEFWENRYLKEDKHELN"
     gene            38946..40082
                     /locus_tag="KR250_001161"
     CDS             38946..40082
                     /locus_tag="KR250_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBH0067125.1"
                     /translation="MKERIIQVADSKKEKILGLCQSLYDEPEIALQEYKSAKKISEFL
                     REEGFDVEENLAGMATAFKATKKNGDGPKIAFIAEYDALPGNGHACGHHLIASMGVGA
                     GIALSSILDTYKGEVSIIGTPAEETGDGKPYLIEHGVFDGYDAAMMIHPNSKTCVTPE
                     IIAIGGLDFIFTGKASHAGAKPYNGINALDAVVLLYNNINALRQQLVDGTRIHGIILE
                     AGTAANVIPDMGKVRLEIRAKEQNYFDEVVEKVKNCARGAAIATGCELEFYHFEPTCQ
                     GLNENKVLVDIFTKIMEEFGIYEDEQVMLGSTDMGNLSQIMPCIHPLMKFSENGEELH
                     TKEFLEASINSYAKDRVIDGIKILALTGFNLFENPELLKKMKEE"
     gene            40318..41433
                     /locus_tag="KR250_001162"
     CDS             40318..41433
                     /locus_tag="KR250_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HBH0067126.1"
                     /translation="METLEVRNIELSGTNYEIGYRLGELVANMPEIIEGQINKSNLVS
                     KKEEKEMIELFDKYCPGLNEELQGFADAIQVNCNQILYYTMTYLKPGCSQVALAPELT
                     ENGHVLFARNFDFSHNMEDFVLCKTKVNGKYAHIGTTVMQFGRGEGMNECGLGVSQSS
                     CGIPVGNSDGLRKPAIVGLQFWAVIRYLLENCKDVDEALEYLKDMPIAYNINLLLADK
                     SGNIALVETLDGKKEVNIINSLESKREYFLHSTNHTHIDKLHKLEPQSMKNSIHRYKL
                     IKEYINKSKKIGEKELMNLLSSKYPNGLSCNYYNDFFGTLKSIVMDLNIGKFNILWGG
                     LENKWESYYLKNDIKSITQRININIEKAPSDFFDFIE"
     gene            41585..42454
                     /locus_tag="KR250_001163"
     CDS             41585..42454
                     /locus_tag="KR250_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBH0067127.1"
                     /translation="MDYLKYFKKAVMYIENNLHENITVNDVAKETGYSYYHLTRLFKS
                     MFGESVGSYIKKRRLVSSTKELLYSDKKVIDIAIGSGFESSEAFSRAFKSIYKVSPIE
                     YRKNRIDVFVGKKKKLELDFMRHLVGNITIKPVIKEIEKIKVIGIKDKVILENNSLPD
                     LWEKFRKVHHIVPNTLPSKRVFGICEAISEIHLVSESMEFNEIIGLEVNSYDVIPNSF
                     VSKTIKEGKYAIFTHTGSLDNLDKTYEYIWGTWFLSSKEELDIRDDFEVYDERFLGPS
                     NVNSQIDIYIPIK"
     gene            42830..43375
                     /locus_tag="KR250_001164"
     CDS             42830..43375
                     /locus_tag="KR250_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HBH0067128.1"
                     /translation="MENVITGLSKGAEWFIGLFQKGGEQFIGLVSGTLPTLIVLMVAI
                     NSLIKIIGEERVNNWASKLGKNSFTRYILLPLVSVFFLGNPMCYTFGRFLKEEHKAGF
                     YDAAVSFVHPITGLFPHANAGELFVWLGISAGLTTLGKETTTLALWYFIVGLIVIFIR
                     GIVTEKMYAFLTRKNRATNKA"
     gene            43388..44401
                     /locus_tag="KR250_001165"
     CDS             43388..44401
                     /locus_tag="KR250_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HBH0067129.1"
                     /translation="MEENKILKIEKGTSGWGGPLYIKKEGNRNKILSMTAGGIHEVTL
                     KIKELLGCEIVDGFKTGVSDEEVAVVIIDCGGTARCGVYPKKKIPTINVNPVGKTGPL
                     AKFITEEYYVSDVNPNCISVVDGEDMPQKSQENKSENKSSIRKPDNYDEVKSKAQGEY
                     AKKNIILSIGQGAGQVVSKFYDAGRDTIQMVMNNVIPFMAFVSMLMGIILASGLGDWI
                     ARVISPLAGNIGGLLIISVICTLPFLSPILGPGAVIAQVVGTLVGTQIGLGAIPAYLA
                     LPALFAINGQAGCDFVPVGLSLGEAEPETVEYGVPALFYSRLITGPIAVIIAYGVAVF
                     ALR"
     gene            44623..46596
                     /locus_tag="KR250_001166"
     CDS             44623..46596
                     /locus_tag="KR250_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBH0067130.1"
                     /translation="MSVTEKIPSIDRKIFKILIMCSKKEFVSINSIANELNVTTRSVR
                     TYIKQLNKDLGNDIAVIKYIKGQGYKLEIKDEQILNKIIDMNRKNVFSLNSKEDRVEF
                     ILNYLIELDGFITLDSLADEMCVGRTTLVNDFQYVEKVLASYNLNLIKKQNTGMKLNG
                     NELDIRLFILNQLYKNSRKDFSNSKYFKGIKKEEIINLEEKLLTLFKKNNFYVTDEML
                     REVINYIVVLVYRVKEEKKVKDYDVKFDLLKSYDEYFIAREIKHIISGMFECTLNDEE
                     IIYLTIPLVSGNAPASECALNSSRISKNIDELMENIFNQIYVDMGIFINEDELRVGLG
                     YHLSFTLNRLLFNIKLKNVLLEEIKQNYILPFKLAQIAGKVIEKKYNLEVSEDEIGYI
                     AIHFSGYLERNSSRFYSIKKIAIICSTGLGTAKLLKIRVEKLIGNNPKIDTLSSFNLR
                     NINLDEYDIVFTTIDLDTSNINTIVLKINTIFDENKLREQLKTVLCLREGNIDTTNST
                     NLLINNLLDEDKFMILNEKTILASLEKMMDHLMDLGCIDDKFRKNIVNREEKSPTVFD
                     KGLLFPHSVNEKSDKFLMAVGILEEPIEYANRSIKIILMTMFPCENKIDSDLLVKIYE
                     EVLKIGQDVKLTNKISKCRTFLEFKKVLLKNLI"
     gene            46665..47024
                     /locus_tag="KR250_001167"
     CDS             46665..47024
                     /locus_tag="KR250_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0067131.1"
                     /translation="MKLLVILFVAYILNTIFIMKQNKLYFKTLNRAKKKGDIVSTGKK
                     KSYFSKGSIAIISSDSDGFIKFGEILKGRTVMAKFKEIEEIEGLDIYSAEQKFKDEES
                     IVQAIKFIKEKINVSFN"
     gene            47059..47415
                     /locus_tag="KR250_001168"
     CDS             47059..47415
                     /locus_tag="KR250_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HBH0067132.1"
                     /translation="MKYEVKISGIGSLVKELMDESNCLIIYDETINDDDLKDISVIHS
                     IATLKSDVEIGDTLTIGNRDYCIVSVGDIAQKTLREIGHCTIKFDGKCEVNLPGEIHV
                     EIGNPDITIGDLITIS"
     gene            47452..48744
                     /locus_tag="KR250_001169"
     CDS             47452..48744
                     /locus_tag="KR250_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HBH0067133.1"
                     /translation="MLDLNRKLAKLEEEGKKIKVALIGAGHMGNGMVSQMANMKGIEA
                     SIVVDINLELAHKAFTDAGIDEEIIDNVTNEKDAEIKLQEGKVLTCNDFLVACKTKSI
                     DVVIDATGGIAIGAEIALNSILNKKHIVMLNAETDCVVGPILKKLADDAGVIFTGSAG
                     DEPGAVMELFDFADAMGFEVRVIGKGKNNKLDLDCNPDTVREEAERKGASPHMIASFK
                     EGTKTMVEMALMCNATGFVPDVRGGHGIEATVNEVPKKYALKSEGGVLDNYGVVDFVN
                     GIAPGVFVVVAHKLKAVNDELKYLSMGDGPNYILYRPYHLCSLETPLSAAMAVLDHKA
                     TIVPKAGLVAEVMTIAKKDLKKGEFMDGYGAYTCYGTIEKYDVAKAMNAVPIGLISKK
                     TKVVKDIKKGEVITYDMVEIEKDTTLYHLRQLQEKIFG"
     gene            49180..49818
                     /locus_tag="KR250_001170"
     CDS             49180..49818
                     /locus_tag="KR250_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0067134.1"
                     /translation="MIIIQLNERQLKIIDIVKENEPITSESIASSLNVTRATLRSDLA
                     ILTMTGILDARPKVGYFYSGVSEINLIGKSVKEKTVEDIMSMPVLAKKDESIYDVIVT
                     MFLSDVGSIVIIDENEELCGVVSRKDLLKATIGGSDINKMPIGMIMTRTPNVVTLTKG
                     ASVLLASRKIIEHEVDSIPIVEYKGEDKNHMRVVGRISKTNITKLFLEIVDN"
     gene            49825..50667
                     /locus_tag="KR250_001171"
     CDS             49825..50667
                     /locus_tag="KR250_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HBH0067135.1"
                     /translation="MLVKNLIIYAVSDSVGETAQQVAKACMSQFYVNETYEIKRFPYM
                     INKGVLLETLENAKAENALIVYTLVDEELCSIVERYCEREGLSCIDLMTDILREISKR
                     TGRKPKREAGIIRKLDESYFKRVEAIEFAVKYDDGKDPRGVLQADIILVGISRTSKTP
                     LSMYLANKNIKVANVPLVPEIPIPKEVFEIDTKKIIGLTNSPEKLNEIRTQRLKALGL
                     SSKANYANLERILQELDYSEEIMKRIGCPVINVSNKAIEETAGIILDIMKENGLKIYK
                     EIEI"
     gene            50728..53355
                     /locus_tag="KR250_001172"
     CDS             50728..53355
                     /locus_tag="KR250_001172"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HBH0067136.1"
                     /translation="METKYVYSFGEGSKDMKSLLGGKGANLAEMTKIGLPVPPGFTIT
                     TEACNDYYVNNESIRAEIIKEIEEHLATLEKDLNKTLGCNKNPLLVSVRSGAVFSMPG
                     MMDTILNLGLNDNSVVGLAEATQNERFAYDSYRRFIQMFSDVAMEVPKYKFENVLDRV
                     KEAKGYTVDTELTTDDLKEIVKEFKAIYKKEIKNDFPQDPKEQLMLAIEAVFRSWNNP
                     RAIVYRKLNDIAHNLGTAVNIQSMVFGNMGETSGTGVAFTRNPATGENKLFGEFLMNA
                     QGEDVVAGIRTPQNISTLADVMPAVFDEFVKITHILEGHYKDMQDIEFTIENERLYIL
                     QTRNGKRTAAAAINVAVDLVEAGIIDEKEAIMRIEPNQLDQLLHPKFEDKALKEAKVI
                     AKGLPASPGAASGKVYFNADDVVKANEKGEKVVLVRLETSPEDIEGMVKAEGILTARG
                     GMTSHAAVVARGMGKCCVAGCGEIKVDEFNKEIRALDDVVIKEGEYISIDGSTGNVYL
                     GDVKKTEVSLTGNFEKLMNWVDKHKCMMVRTNADNPRDARAAIEFGAEGIGLCRTEHM
                     FFDEARLPAVREMILSNTVEQREKALEKILPMQREDFVELFKVMDGKPVNIRLLDPPL
                     HEFLPHDDETIEELSKSMGIKVSDIKKRIVDLDEFNPMLGHRGCRLAITYPEICVMQS
                     KAIIQGAIEAIKAGVKVSPEIMVPLVGEVNELKIIRKMIVETVDAIIKEEGIEVPYTV
                     GTMIEIPRACLTADEIAQEADFFSFGTNDLTQMAFGYSRDDAGKFLGQYVDEEILEKD
                     PFQVLDQNGVGKLVKMGAKLGREVKPELKLGICGEHGGEPSSVEFCYSVGLNYVSCSP
                     FRVPIARLAAAQASIKNPR"
     gene            53569..53814
                     /locus_tag="KR250_001173"
     CDS             53569..53814
                     /locus_tag="KR250_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBH0067137.1"
                     /translation="MTKCPKCGKEVSSRPGSICPRCGFKVADESLKIRCPEPSCRALV
                     SRKLDYCPKCGCKLRGYNISEFVNMVRCKIDETFDKK"
     gene            complement(54103..55401)
                     /locus_tag="KR250_001174"
     CDS             complement(54103..55401)
                     /locus_tag="KR250_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HBH0067138.1"
                     /translation="MSKIVAISCGVGIITANLIPVYADNNTSSINKVDNSILSDSNKR
                     TSEKEILTAREAVSAALKNSEKLKMKSEEIKMLKEKLEVQDEFDSFTGSDNSFPYDQI
                     GLLKNQSEQAKGFMEDQIANDITNKFNDLVSRENELDKIKNNLEIKTKEIKDMKLKKD
                     LGLVTSLETESAELELQTLQNTQKAKLQELKNNQDYFKLLTNIDLNNYQLDKEYRFES
                     FRVGGSVDSYMEGKVNEYLKYDQLILERTEESFNDKDENKADLPDRPNLTRPVAPTKP
                     EQGNLTDDEYKVLMDKYEKDYDRYKKEVEAYNLERQTYAAGLTTYANYLQQKFNTENG
                     LVTLEDSKKALKKGLIDSYAQLLALEDTIQITKKQLDLSEKQLKNTKLRYDLGLITLT
                     DYKKQVVSNEDAKNSYDTLIVNYNSLKNGIEKPWILNTGK"
     gene            complement(55435..58569)
                     /locus_tag="KR250_001175"
     CDS             complement(55435..58569)
                     /locus_tag="KR250_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HBH0067139.1"
                     /translation="MNLTQTSVKRPLTIIMVFLVLIVFGGIGYKKMSINLMPDIEIPV
                     VMVMTTWTGAGPQDVDEQVSQKVDESLSAVSNVKSTISSSQESVSMVVAQFEFGTNLD
                     EIMNDVRSKVDALQTSLPDDAAKPTVLKLDMNAQAIGQLVISGGNENSSQALRKYAED
                     VIQPKIESIDGVTSADLKGGEKAQVNVIADPAVLSNYGVSLSTIKGVLSSSNKTFPYG
                     SITQGEDKIVLRAIDKLESLDDIKQIQIPVKGGNTVRLDNLCSVDYGYADKDSIYRYN
                     GKDSLVIDISKQQDANTVKVMQSAYKYVDELNKENPQFKIKIANDTSSYISESINSVM
                     SNLLISAVIAFIVIFAFLKSVRASLVVAVAIPISIIGAIAILYFTGESLNLITASSLV
                     ISVGMVVDNATVVIENIFKYRKSGRLSLDDCAIEGTRTVTNAILASTLTTIAIFLPIM
                     FTEGIAKITFGALGKTLIASLAFSFIGAITLVPSVFAKLSREKNSQKMQEKDSPIFDK
                     VSEAYKKLLSVCLRHQRLVVISSLLIFVASLFGMGFIGMDFMPAADKGELSISIELPK
                     GLSLESNDYYVSMTEKKVSDIPEIKTLITTLTSDSNNSKASIAIELESQKNRKRTTDE
                     IEKEIIDRVATVPDCKINVSQNSSVMGGAGGADFTLEMKGPDLDVLKILGKEAEKEFS
                     SIKGFRNVETSLTDTTQEAQFTIDKHKAQEYGVNTAEIAGMLRTAINGDSVTTATIDD
                     YKVDVNLKFKADTIDSLEDIKQLKVNSATGKEVPLGAFSDIKMADGLQSISRTDGDFS
                     VNITATLDNLDAGTATKLALEKTNKMVIPKDYSIGVGGETEMMNESMASLGFSMVIAI
                     ALVYMVMVAQFESFSKPFIIMFCVPFAFVGVVLSLLVSGQSLSTVGMLGIILLIGIVV
                     NNGIVLIDYIEQLRKTNRNNDLIGLVSIGCAARLRPVLMTTATTILGMLPTALSLGEG
                     GETMQPLAIVIMGGLTVSTLITLVLIPTIYLIFDKWENKFSDKMPKIFGKITDKIGSI
                     KRKIIKKPKLKAKV"
     gene            complement(58573..59796)
                     /locus_tag="KR250_001176"
     CDS             complement(58573..59796)
                     /locus_tag="KR250_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HBH0067140.1"
                     /translation="MKRYLIVFLMCSSLFLVGCGKTEESEPEKPIAVSVQKAIGGEIE
                     NTNSFSGTTKVKDETAVTAQTVGTVQEVYVKLGQNVRKGDELLSISSPELENSVKQSK
                     ASLDLAKASYSSATGGSLEAQVNQAKTALDNAKIQYEESQRNYDNNKILYEQEVISLD
                     QFKKIEFSLEQTKQQLDSAQRAYDTATSKSIPQAKALAKKQLDQAQVSYNLAMSNLDK
                     LTLTSPVDGTITAKNFDSKEMITQSQPAFIISNPNILEVDLNVAESDIGKFKKDGNVD
                     VIIEDQRILGKIDYVPSVVDPQTSLYPVKVLVNNTNNKFKAGMSAQVNLSVEKENGAV
                     TVPKKAIFEENGKKYVYIATKDNIAKKHLVQTGIVTEDKIEIKSGVSDKDTVIIGGIS
                     LISDGTKIFPVEKEK"
     gene            60048..60533
                     /locus_tag="KR250_001177"
     CDS             60048..60533
                     /locus_tag="KR250_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBH0067141.1"
                     /translation="MDNNNILKIADSFLDFLFVLENNIFKESDLLKKFQNNSDIMKAY
                     FEECPMAPSHAKVILYLMTSNSSSISQIASNLGILKSNMTPIIDRLVEHGLVNKFPDP
                     KDRRILRVELTDKAFKLFDAVKAILKESLVKKLSNLSEEELTLLDEHTLKLSEIVKKL
                     G"
BASE COUNT        23535 a         6640 c        10768 g        19631 t
ORIGIN      
        1 aaagttttat atctaataag gtcaaacaaa tttaaaaact tattttcagc gttgtctttt
       61 ataatatgag gtagtaaatc agaacataac tgtaatctaa aatcaaaatc agctttacta
      121 tctaattcac caagataaaa aatataagta ctttgataag tttcagtttc aattatattt
      181 tgtgtagtta cattaatatc taagttatat ttagaattta ctataaaaaa accattgtgt
      241 tgtattatat ttatatcttc aaataaatta taaaaaatat tttttagata ttcaaagtct
      301 atatgagatt ggtaaattaa ataaaagttg ccttttaaat tatcgagatt atttttatct
      361 agaaaatcaa taacataaat agcattattg aaagtaacta aatggctact agcatctaac
      421 ttttggtttt catatggata gatatttatt tttttattaa gctgtctttc taaaaaatct
      481 tttaactctt ccataaagtc tccttaaaaa taagattata ttcttatctt atagctaaag
      541 tagtttcttt gtcaaatatg tgtactctac tattgtcaac tgaaagtgtt aagtcatcac
      601 caacattaat tctgttttta ctgtcaaatc taattgttat cgtattttcg cctagtttta
      661 agtgagcata agtttctgct cccataagtt cttttatttc aattcttgat ttaaaacaag
      721 tatctggatt agatttaata aaagtatctt ctacatgaat atcttctggt ctgataccta
      781 taacaacttc attattaata taattgtctt ttattaaatc atagtgtttt tctttattca
      841 atttaagctt aatatgttca ttttgtgcat aaatttcatc attttcttct attaatatta
      901 catcaataaa attcatctgt ggtgctccaa taaatcctgc aacaaatata ttagctggat
      961 aatcgtatac atcttgtgga gttgcaatct gctgaactac tccatccttc attactacga
     1021 ttctatcagc catagtcata gcttcaactt ggtcgtgtgt tacatagata aatgtagttc
     1081 caagctgttg gtgtagctta gttatctctg ttctcatagc agttctaagt tttgcatcta
     1141 agttggatag aggttcatcc attaagaata ccttaggatt tctaactatt gcacgtccta
     1201 aggcaaccct ttgtctttgt cctccagata atgttttagg ttttttattt aaaagaggta
     1261 gtaagtctaa tattttagca gcttctttaa cttttttgtc tatttcatct ttaggcaatt
     1321 ttctaagttt aagtgcaaat gccatgtttt cgtaaacaga taagtgggga tataaagcat
     1381 agttttgaaa aaccatagct atatctctat cttttggttc tatatcattg acaagtttat
     1441 ctccaatgta tagttctcct tcagagattt cttcaagacc agcaatcatt ctaagtgtag
     1501 tcgatttacc acaaccagat ggtcctacta aaacgataaa ttctttatct ttaatgtcta
     1561 tatttatatt ttttactgca ttaaatccat ttgagtaaag ttttgatata ttttttaaaa
     1621 ttacttctga cataatatcc tccttgatat ttaaatttta attatatagt atcaaatttt
     1681 tttattatat taaatgtgta aaatgactaa atgtatttgt ttaatatgta tagttagttt
     1741 ttatattaag atttattata atttagaaaa tatattttaa tttttttaat ttttatgtat
     1801 aataatatat atatttttta gattaacaat atgggattta aattattttt taatttataa
     1861 tgaaataata ttatattagt agttttggtt aacaatgagg agtttataaa taaggtcata
     1921 taagttaaat tatatcgatt ttatttaaat aaccccatat ttattaatta ttagaaatat
     1981 attttaatta ttgactaaat aataaatact gtaatataat ggaaatacag tttaataaat
     2041 ataaaaagag aaagagggaa ttttaaatga atttgaagaa aaaagagaaa atagttaagc
     2101 ctgttttaaa gaagcttaca gaaatagtat taaatatatt atcaaaaaaa atattaaact
     2161 atgctatata tgcaatttct gttttgatag taacaaaagt tgttataaat atagatttgt
     2221 acatatccat attgtattat tagatatctt atagatataa aaaattaata gatatatatc
     2281 tattaatttt ttatattaca aatagagaat aatgaattat actagaaagt aggaggtgaa
     2341 aatatgagca gaatttcaat ttttatggaa tcatgtaatg gcaaatttcc aaatgaaaat
     2401 tcaatgttgt cagtttcctt tataaaaaat attttaaatg cagaaaacaa agattttgct
     2461 aaaaacatat ttaactatgg ggaagaaaga aaaaacaata agcaaataaa ggatataaat
     2521 acagccattt acataccaaa tcttataaga agcaaaaatg agttacttgt tgatggagat
     2581 ataatattca atataagttt ttataattat tctatgttca gtaaattata caatggaatt
     2641 ttaaaagtaa aggagctaga atataaaggt tatagattta aaataaaaaa tataaaaata
     2701 cataaagaac acgagataaa agaaaatggt gttatattta aaactatgtc tcctataata
     2761 gtaaagaata aagaaggtaa atatttagat gtagaagatt caaattatat agaatgttta
     2821 aactatatag ctaatctaac tctggagagt ataagaggta gtgggcttag aaagccattg
     2881 gaatttatac ctcttaactt caaaaaaaga gtgttaaaag aaaagattcg tggatttaaa
     2941 gagagagaat tttactatat aaatgcttat gcaggaacat tttttttaaa aggaaatatg
     3001 gaagatttaa atgcactgta taaaatggga attggatata gaaggacgga aaatgcagga
     3061 atggttgata tattaaagta gcgaggtgag agaaataaaa gctaaatttt atagatcagg
     3121 tgatattgta agagatattg gcattgttta cttttgtgaa ttactaatgg atttaaaaaa
     3181 cgaactagaa aaaaatgatt ataagctaaa atttacttgt gagttgaata gaaattattt
     3241 atctttagaa tctgcagaat atatagaccc taagtatata tctgattata tattagaaaa
     3301 tcaacttttt aaggtattta aaaatgggaa aaaggaaacc ttagaaaaaa tatttagtaa
     3361 tatagataat ttaacatatg taaattttat tgaagaattg gataaatctt ctgcaactga
     3421 gaaacaaaag gaagggatta aaaaagattt taaaaacaga agatttccat atgttagaaa
     3481 tagtggtaag tatggattaa acacgagtct agaaaatatg gaaaaaaatg taaaaagaat
     3541 agtcgatatt gtgattaaat caaatataga gatagatgaa atagataaac ttgatttgaa
     3601 acaatatgaa gaaaaagata gtgtatgtaa tgtttgcaat ataaataaaa caacaaaact
     3661 agatattgat ttaagagaac gaaaggactc taagtataac tttttattta gaggtgcaga
     3721 aaaatcaggt tttaaaagaa gtggacaagt tgaaagtaat atttgttttg agtgtgaatt
     3781 tttcaactta atgtgtcttt tatatataaa tttaaaaaga ccaatggttt ttgcatatac
     3841 gaatgattta agagaattag catttttaaa tcataagata atgcttaaaa gaaaaatgta
     3901 ttcagataaa tcattttata aaaaactttt gcatgaaaaa ataagttcaa ttagactata
     3961 taggattgat attgatacga ataaaggtat aattctaaaa tttgactcta ttatagagta
     4021 taaagaatta ttaaaaaaaa ttgaacttat agatattata gataattata atttttcaag
     4081 agagccaatg aatactagaa atttgggtaa aaaaatgatt aataatggga atttatcaaa
     4141 tttaaaagaa ttattacttg ataatataag tgttttaagg caactaactg gaacttctcg
     4201 agaaatggat tttgtatcta gttcatataa tatcggttta tatataaaat tgtgttggat
     4261 tgtagatgga agggaggagt ataaaatgaa aaactatata gaaagtaatt taatatattc
     4321 aagagtaggt aaagacttat ttaataaaat gacagatgaa tccagaagaa aaaatttttc
     4381 tatgaaagtg attcaactac ttaagtctaa tgatagaact cagctatttc aaactataat
     4441 gcatgtgtta gtttctaatg gaatcttaat aggagaaggg tttgtagaag gtctaatgca
     4501 gagttctgaa ttagaattaa atacgaatgt aggtctattt attcaagaaa tcatgaaata
     4561 aaaaaggggg aaaatttaaa atgaatgaaa aaagagtaaa aggagtaaca ttaactatac
     4621 tgacagaaag cccagttcca ctttcgtatg accaagggta tggaaattat acaccaataa
     4681 aaaaagaaca atatagagat aaagtacatg caaaaactag tatagctaca ataacatatg
     4741 atttaagaag aatactccat caagaatatg gttggaattt aagtaatatt gtttttggaa
     4801 aaaaaggaaa tatatatcca tctattaaga aaaatgtagg atgtgtagat gaaaatggat
     4861 tagagactga tgtatttggt tatttaatac cacttaaaga gaaaggttct ataagtaagg
     4921 cttctccgct tagaataata ccttttaggt cactaaattc atatagaggt tcaacacagt
     4981 tgataacaaa tagaggtttt ttaagtagcg aatttggtag aaaatattat gatgagaggg
     5041 aagaaaatga agtacttaga gatgaaaact ttccaacaac acaagcatta gcaatagaag
     5101 agactttatc agattactac gtttatacaa taacattgga gcttgataga ataggagtag
     5161 tagaagtaga agaagatgga aagttacttt taccagaaga aagaaaattt atgagtaagg
     5221 agttaagaga aaaagcagta aaagatatat tagatgcaat tactgtgttt acaagaaaca
     5281 taaaacattc atcagtacta ttaaaaccac ttgcagtaat gggtggagct tttgataaag
     5341 ttgtaccatt tttttgggat gatgtagatt ataatgctga tagtggtgag ataaatttag
     5401 agggagtcat agaaactata gaaagttata gccttaaaga aagtaacaca atacttgcta
     5461 tcaatgacag attaaaaatt tctaataaaa aggaattaaa taagtttaat ttaagtaaat
     5521 accctgtaaa agagattaaa aatctagcag atagattgga aattggagaa gataatatgt
     5581 ggtatttaaa agagtaggag gttttttgaa ttatgaaagt attacaatgt aaacttaagc
     5641 aaccaactgc tcactataga gacccaaaag tatttcaaaa tgaatatata agtactttaa
     5701 atttaccatc taagaccaca ataatgggaa tgataacgta tttatgtgat agaagattaa
     5761 actctgatat agatatagga ataattggaa cacatcatca tagagaacta gaattttcaa
     5821 gaggagagaa cattgatttt tggaatgaat atacaaatat gagaaaaggc aaagataaag
     5881 aaaagttttt gcttcaagga aactactatg attactataa agaacataaa gctcaaaata
     5941 gcatattaaa ctatgaagtt cttaaagaag tagaactgac tatatttatt tcttgcaagg
     6001 atgatgaaga acttgaattt attagaaaaa agcttgaaag tccatgtaaa tatgcaaatt
     6061 taggaagaaa agaagatttt gtaataccaa gtgaaaaagg gtgttttgtg aaagaagttg
     6121 agttaaaaga ggttatacct atgaatactc gtgatgctat aaaagaaaat ataaaattaa
     6181 agaatacata tgtaagagtt gacttaagag ataaagagaa tgtcgagact ataataaatc
     6241 aaggtgtatt gattgcatta ccttataaat ataaagattt agaagcaaat agagatgata
     6301 gagtttatga gttttgccat tatatatatg ttgataatga tggtatatat cctaaaaata
     6361 ttaaggtaaa tgtatgtatt gatacaaaag aagtttttac atggctttaa attattttga
     6421 aagataggtg tttatcatga aaaatatcat gcaaaaagaa atattagcta agtcatataa
     6481 tttaagaact aatcaacaag agcaaacatt agttgaacat attaaggatt tatttgaagt
     6541 attagaatct atattagaac taaatctata ttcagataaa gatgtagaga tactaaaaat
     6601 atgttgtgct ttgcacgatt taggaaaaat taattcaatt atgcaacaaa aaatggagat
     6661 taataataaa atatcgtata gttgctcaga agaagagagg aaaaagttag agtctgacaa
     6721 aaaatcttta aaaaagatag caagacataa tatattttct ggagcatttt taaaagatat
     6781 attagaaaaa atgaacttgt cagaagaaga taagttatat atatataaaa gtataatgtt
     6841 acatcatgga aactatgaag attatatgag gttaagtatt agtaaagttc aagaagaaat
     6901 ttatgagtat attgaaaaag gcattttaga aaaagaagag tttaatctga aagatataga
     6961 aagttatata aatagcattt taaatgtaga ctttaaattt ggtgaagatg tattggatta
     7021 tgattacata gataaattaa gtgaaagtat gtttatagac agtgattata acaatggtca
     7081 aatagatgat tcagttttaa attatagaaa gtttaagtat attttatata aagggatgct
     7141 gaacttaata gaccatagtg catcaagtag acagaaaggt ataaaatttt ataacgattt
     7201 tactgatgaa gaaattgata atatgatttt aaatgagata tataagagtc agggtaattt
     7261 ggaaaaaaat aatatagagt tcaatactat acaaaaaagg ttgagagaac tttctggaag
     7321 aaatgttctg actgttgcat ttacaggttc aggaaaaaca gctgcagatt atagaaagac
     7381 ttttaaaaga aaattctttt tagtgccaaa taaaatatca gcagaaagtt tttatagaaa
     7441 aaatattttt caaaataaaa atgatttaga tacaagaagt agtaatgatt acattggatt
     7501 acttcatgga gatataaact tatattcaga gaatgaagat aatggagaac atgattttgt
     7561 attgacatta agggatatag atttgagtat aaacttttgt aaaccttgtg taattgcaac
     7621 agttgaccag ttattactta gtatgtttaa atttccggga tatgaaaaaa tattcgcagc
     7681 cgtaaaaaat gcatctataa ctgttgatga agtacattta ttggaaccaa agatgttttt
     7741 gattttgata tattttatgc agtttgcatg caagtactta gatgttgatt ttcacttaat
     7801 gacagctaca cttccaaaat catataaaga gcaaatgata aacaagggaa ttatttttca
     7861 agaagaaagt aatgaaaatg ttactgataa aggagaaata gtttttattg agagcaacaa
     7921 ggaagaagag atttgtgaag gtaaagatgt aaaggtttct tttataaagg aaaaagaaat
     7981 caagtcaata gttgaagatg ctttagaaaa taaacaaaaa attcttataa taaagaatac
     8041 tattgattca gtcaatagaa catatgagtt tttaaaagaa aatttatctg ataagtatgg
     8101 tggtgtagat atagatgttt tacatagtag atttaagttc aaagataaaa aagagaaata
     8161 tagtaaaata ttaaatggag agggtgatat ttggatatca actcaatcag tagaaatatc
     8221 cttggatttg gatttcaata taataataag tgatttagca actatggata gcttgataca
     8281 aagaatgggt agatgtaata gaaataataa atatgaatat ggaaactttt atattctacc
     8341 tagtgaagat aaaatttatg ataacaaact taagaacaca actaaaaaca tcttaaagga
     8401 catattaaaa actaactcta tttttacaat gggaattaga aaaactattt tggatgatta
     8461 ctatgataat agtgtagtca aaaaatattt tgaagagaat tttataagtt gtgataaaga
     8521 aattaagaat atttatggta taaataaaga aatttttgat ggattggatt taatttttaa
     8581 ttttgaacct tacaaaaata ttgttgatag taagaaggaa gcagctaaaa tttttagaga
     8641 tgttgatgtt agttacaaaa ttattctgga agaagatttt tataaggaag atagagagtt
     8701 acaacaagat tcgattcaag tatcagggtt tatatttaat agattatatt attatgggtt
     8761 aataagcaaa gtagaaggat acatggtact aaaatctagt agtaagtttg agtataatag
     8821 tactgtaggc ttaattttga agtgagcata tgtttaaata taatatacct ttagacattg
     8881 aaaatacaaa aagtactact gtaagctcac tgtaaaaact atttttttaa aaggaaaaat
     8941 aagttgtgag atagagtgtt tacaagtgta tatagataat aaatttgagg tttaagatta
     9001 actaagtggg tttcaggtac atcgcgaaaa taattataag ttggagattt tgtatgttta
     9061 agattaacta agtggatttc aaatattgat atagtagata taatagaagt tttattatta
     9121 ggtttaaaat taactaatcg gatttttaaa tattctgact attatataag gatattatta
     9181 gcttgaattt aaaaccaata atataaaaca acaaaatatt atatttatga tataataaaa
     9241 gttatggaaa aacaggaata ttactataga gaagagaggt aaacttatga ataattggat
     9301 agaagtaact ataaaaacaa caacagaagc agttgaacct ataacgaata tactatatga
     9361 acaaggagct ggaggagctg taatagaaga cccaaaagac tttttattcc aaaagaaaaa
     9421 tgaactagac tgggattatg ttgaagaaga agtatttaaa aagaatgaag aagatgatgt
     9481 tcttataaaa acttatgttt ctgaagaaaa aaatgtgatg gaatttgtcg aaataataaa
     9541 gcaaaaagtt ttagggttaa aagattttgg tatagatata ggtgaaggaa gtgtatcatt
     9601 ggaccaagta aatgaagctg attgggcaaa tgcatggaaa gcatactaca aaccaacaaa
     9661 agtaggacaa agggtagtag taaagcctac atgggaagat tatgctatgc aagaggggga
     9721 tttaatcata gagcttgacc ctggaatggc atttggtaca ggtacacatg agactactag
     9781 tatgtgcatt agagaattag aaaagtacgt gaataaagat tctaaagtat ttgatattgg
     9841 ctgtggtagt ggaatccttg caatagcagc agctaaatta ggagcaaaag aagttgtagc
     9901 tgtagattta gatgaagtgg ctgtaaaagt tgcaaaagaa aatgttcttg aaaataaagt
     9961 tgaaaaaagt gtatctgtaa tgcatggtaa tttaactgat gtaataaaag ataaagcgga
    10021 tgtaatagtt gcaaatataa tagctgatat aataaagata cttgcaaaag atgttcaaaa
    10081 ctttatgaaa gaagatgcta tattcatatc ttcaggtata attttggata aagtagaaga
    10141 agttaaagaa agcttaattg aaaatggatt tgaaatagtt gaagttcaaa aattaggtga
    10201 atggtcagca atagtttcta agcttaaaaa ataagggata tggtgacgta taatggatag
    10261 attttttgtt gaaaaaaata atataaatct acaagataaa acttgcacta tagaaggtga
    10321 agatgtaaaa catatttcaa aagttttaag atgcaaatta ggtgagaaac ttgaaatatg
    10381 tgacaagaat aataatgagt atatttgtga gataatgaat atagataaga gtatagttaa
    10441 tttagaaatt ttagaaaaag ttgatataaa tagagaatca gaacttaaag taaggctata
    10501 ccagggactt ccaaaagctc caaaaatgga aatgatactt caaaaattaa ctgaagtggg
    10561 tgtagaagaa ataatattag ttcaaactaa gagaagtgtt gttaaagttg atgacaaaaa
    10621 agaagataaa aaatttgaac gttgggaaag aataatttat gaagcagcaa agcaaagcaa
    10681 aagaggtaag ataccaaaat taagaggtgt tttaagtttt aaagaagctt tagaagatat
    10741 gaaaaaaaat aatgtaaata tttgccctta tgaaaatgaa agaacagttt caataaaaca
    10801 tgcactaaaa aaatgtgatt ctaatataga tagtgttgga atttttatag gtccagaagg
    10861 tggattttct gaagaagaaa tagaacaaat acaaaaaaat aattgtaatg ttgtatcatt
    10921 ggggcctaga atattaagaa ctgagacagc ttctgttgta gcatcaacaa tagctttgta
    10981 tgaactaagt gatttaggag gagaaaaata aattgaaaaa agtagctttt tatacacttg
    11041 ggtgtaaagt taatcaatat gaaacagaag ctatgctaga actatttgag aaagatggtt
    11101 atgaacaagt caatagtgaa gagtatgctg atgtttatgt tataaataca tgtacagtta
    11161 ctcatatgag tgatagaaaa tctcgtcaat atataagacg agttaaaaag aagaatccag
    11221 atgcaataat agccgttgta ggatgttatt ctcaggtatc tccagaagaa attttggata
    11281 tagaagaagt aaatttagtc atgggcacaa atgatagaag aaagattgtt gaagaaatta
    11341 aaaagataaa ttcaagtaag aaagttagta cagttgatga cataatgaaa gttaaagctt
    11401 ttgaagaaat agaaatcagc caaactaatg gaaagactag agcatttatg aaaatacaag
    11461 atggatgtga cagattttgt acttattgta tcattcctta tgcccgagga cgcgttagaa
    11521 gtagagatat tgatagtata gtcgatgaag tgaaaaaatt ggctaataat ggctataaag
    11581 aggttgtatt aacaggaata catgttgcct cttatggaaa agatttaaag gatagggata
    11641 ttaaacttct tgatgtaata aaacaaataa accagataga aaaaatagaa agaattaggc
    11701 ttagttctgt tgaaccaatt ttatttacag atgaatttgt aaatgaagtt ttaaagatgg
    11761 ataaagtttg tcctcattac catttatcac ttcaaagtgg atgtgatgaa actctaaaaa
    11821 gaatgaatag acgatacaca actttagagt acaaaactat tgtagataga ttgagaagta
    11881 aaatgccaga tgtggcaata actacagatg taatagtagg ttttcctgga gagacaaatg
    11941 aagagtttaa aaaaacgtac gagtttttaa aagaaataga gctttcacaa atgcatattt
    12001 ttaaatattc accaagaaaa ggaactccag ctgcaactat ggaaaatcaa gttgaccctc
    12061 aaatgaaaca ctttagaagt gaacaacttt taaatttaag taaagtaaat tttaataaat
    12121 ttgcaactaa atttatcggc cgtgagttgg atgttttatt tgaacaaaat atagaaggca
    12181 ataaatatga gggattaact tcaaattata taagagttgt tgttgaaagt gacaaaaata
    12241 tacaaggtca gatattaaaa gtgaaaataa atgatgtaaa agatgaatat gtagaaggaa
    12301 ttttgctata acatgtagaa attataatat ttataagata ggaggtggaa agaatggatt
    12361 gtattttttg taaaatagcg aacggagaaa taccttcaac taaagtttat gaagatgata
    12421 gagtcttagc ttttaatgac ttgaacccag ttgctccata tcatatatta gttgtaccta
    12481 aaaaacatta tgatagttta attgatattc ctgataaaga gatggacatt gtgtcacata
    12541 ttcatgttgt tattaataaa atagctaaag agaaaggttt tgaccaaact ggattcagag
    12601 tcataaataa ttgtggaagt gatggagggc aagaagttaa acatttgcac tatcatattt
    12661 tagctggtaa aaagcttcct aattacgaag cgggacaaaa ctaaactaaa ttattgaaaa
    12721 attgagttcc ttcttgataa aaatacttga taaaagtaaa aatttagttt ataataaaca
    12781 aagtgttagg ttgtaatgca tattatgcaa aaagaacata gttacagtcc cagcataatt
    12841 gcgctattgg agggagggaa aaagaaatgt cagaagtaag agttagagag aatgaaacat
    12901 tagacagcgc tttaagaaga ttcaagcgtc aatgtgcaat gtctggcatc atgtcagaag
    12961 tgagaaaaag agagcactat gataagccaa gcgttaagcg taagaaaaaa gctgaagcag
    13021 caagaagaaa aaacgctaag aaatagtagt aaataagaat aaagaggtga aggggatgtc
    13081 ccttaagcaa aagttacaag aggatttgaa atcttcgatg aaaaacaaag atacagtgag
    13141 aaaatctgta gtaactttaa taagagcttc tataaagcag tatgaggttg ataatagggt
    13201 cgagcttgat gaagatggaa ttatcgatgt aatagcaaaa caattaaagc aacgtagaga
    13261 tgcattagtt gaatttgaaa aagctggaag agaagactta ataaaagaaa cagaaggtga
    13321 aattgaagtt ttaaaagagt acctacctca acaattaagc gaagaagagt tagaagaaat
    13381 tgtaaaatct actatatctg aagtaggggc aacttctatg aaggatatgg gaaaaataat
    13441 gtcagttatc caaccaaaag ttaaaggaag agctgacgga aaacttataa ataagttggt
    13501 aaaacaaaac ttacaataga ttgatatagc ctagagagaa atctctaggc tttttatagt
    13561 ttttgggaat aacgagtcat atttgagcat atctataatc aaagaaaatt ttgaaagaag
    13621 gggaaaaatt tgattatatc agtaaaagag attaggaatt ttttgataaa catatttata
    13681 ccactaataa taggttactt aagtagcatc ttaacaatga ttatcagtgg aacagatatt
    13741 tcaacctatt atttacaact cacaaagcca agctttgcac caccagcttt tatctttcct
    13801 atagtttgga ctatattgta catattgatg ggtatatctt catacctaat attaagaaag
    13861 ggttacaact tacctaaagt aagagatgca attttttatt atggattaca acttgtatta
    13921 aactttattt ggagtatact attttttgga tttggactta gatttacagc tttgatagat
    13981 ataataataa tgattttggt ttctataata atgatttcga agttttctaa aatagataag
    14041 agatctggat acataaattt gatttattta atgtggcttg tttatgcagg atttttgaac
    14101 tattttatat ggtttattaa taaatagaat gtaaagattt tgtaaattac cctgtacttt
    14161 taataatatc ataacaaacg aaaaataaat agactattaa aaataatcat tttcatatac
    14221 aataatatta atttaaatac aataaattta taaaaattgt atttaaagat taacatatta
    14281 aagtattaga attggaaagg cttgaaggga gaggttttta tgcaaataat agtatctggg
    14341 agacaaatga aattaactga cggaataaaa ggatacgtag atggaaaatt aagtaggtta
    14401 gaaaaatacc ttgatcctga atcagaagtg aaagtaacag taagtgcgaa aaaggataga
    14461 caaaaagtgg aggttacaat aattcctatt aacggtcaaa taataagagc agaagacgta
    14521 gaagatgact tatatgcagc aattgatatt gtttgtgaca aattaagtag acaagttgtc
    14581 aaatataaaa ctaaagttaa agacaaagtt caaaataata agagcataag atttgaaaat
    14641 cttgacttta tagataatag cagtgaattt gatgattatg attatgatga tgaagaagat
    14701 gagaatatag taatagaaag aagaaagaaa tttaatgtaa aacctatgag ttctgaggag
    14761 gcgatacttc aaatggaact agtaggacac aatttctata tgtttagaaa ccaagataat
    14821 tttgaaataa acatagtgta taagcgtaaa gctggtggat atggtttaat agaacaagat
    14881 taataaaaat tgtatacgtt tagattttat ataagaaaaa ataaaaaggt aatcatgttg
    14941 attttttatc aatatgatta cctttttctt gtttaaataa tatattagat attaggtatt
    15001 tattatataa aaattttaaa actaatctgt tacaacatca ataaagtgca taaataccta
    15061 tttcaagcat aaaattaatt aagggggtaa tgcttatgtt agaaatatcc actgatttac
    15121 aagtaaatca accagttatt actgttacaa gtaatacgtt tattagtata gaaaattatc
    15181 tatcaatatt agaatatgaa gttgatttaa taagaataaa gactaaagtt aaaacaatta
    15241 aaatatctgg ggataaactt tcacttaagt atattactga tagtgagata ggtataaaag
    15301 ggattatcta caatgttgag tatgttgatt aggaggagat acacttgata agttttataa
    15361 ggggatacta tgttatagtt gtggaaggag taggactcga acaattttta aatcatctta
    15421 taagaaatgg gataagtgtt tacaatgtaa ctagaataaa aaatactaaa atggagtttc
    15481 atatagatag acaagatata aaagaattta agaatgttta tagaggaagt aagtttgata
    15541 taaaggtaaa gcaaaaaact ggggtccctt ttattatcaa aagagtttat aagcacaaag
    15601 gtatgtggat ttgtgctctt gtttctttat ttttattgat gtctacatca cagtttgtga
    15661 cagatgtata tatacaatct cctgaaggta taaaaaaaga agctcttaga aatgagcttt
    15721 ataaggttgg agtaagacca ggagtctata agaagagtat tgatagaaaa gaagttagag
    15781 accatatgat gtcaaaattt aatgatgtgg catatttatc tatcaatgta aaaggaacaa
    15841 atatatttgt tactgttaca aaaaaggcag aatccctaaa atctacagac caatcaaatt
    15901 attgtaatgt aattgcttta aaaaatggta taatagaaaa ggtaatccct agaagtggta
    15961 aatcagttat taaatctgga gacatagtcc aaaagggaga tgtcttatta aatggagcta
    16021 ataccaagtc aataccagaa gtatgggcaa gtacttttta tgagtccaca aaaaaggcaa
    16081 gttatgtaga tactgtaaat aaaaaaactg gagaaaagaa aaatatatat acactaagtt
    16141 tttatgataa agagtttaca atgagaaaaa atataaaata taaagattat gttgttgaaa
    16201 ataaagaaaa aaaactatca ataggaaact atacttttcc aataaagata aaaacgagta
    16261 ctttttatga aacaaaaaag gttgaagtaa aaaggaataa agaagagtta aaaaaagaat
    16321 tatctgaaaa agctttaaaa gagttagaat acataatccc agcttcggct agaataatag
    16381 atgtaaaaca taactttaag gttaataaaa atatgttaga atatttaata acagttcaaa
    16441 ctagtgaaaa tattgctaaa atttatcctc ttagtaagtc agaagcagaa aggtttataa
    16501 aggaagaaag taaaccagat gaaggtgaag aggaagttcc ttccaatcca gaaaaaagac
    16561 cattaaatga tataagaaat gagtttgatg aggataataa ggataataaa gatcaaaaca
    16621 attctgatga aaataatagt aatcaaaata gtaataatag tcaaaataat aatagcaata
    16681 acaactaata aaattaaact taaaatctta ggaggactaa ttcaattgat aatacaaaag
    16741 aaatttacaa tagcagatgg taactttgaa agagagttat ttggcaactt tgatgaaaat
    16801 gtaaaattaa tagagaagac tttaaatata gatgtaatac ttagagaagg caatataatt
    16861 ttaattggag aagaaaaaaa tgtagattct gcattaaaac ttatgaatga attacaccaa
    16921 actgtatcaa atggtaagca tttagataag cagagtattt cttattcatt atctcttttg
    16981 cttgaaggaa gtgaacaaaa aataaaagaa cttgagggga caatcgtcat aactcaaagg
    17041 ggaaaagctg ttcaacctaa aactttaggt caaaaagaat atataaaact tatagaaaat
    17101 aatgatataa cttttggtgt tggacctgct ggaacaggaa agacttatct tgcggttgct
    17161 atggcagtaa aagcttttaa aagagatgaa gttagtagaa ttatacttac aagaccagct
    17221 gtggaagcag gagagagtct aggtttttta ccaggagatt taaaggacaa agttgaccca
    17281 tatttaagac cactatatga tgccttattt gaaatgttgg gtgctgataa atttaataag
    17341 tatttagaaa gaggtactat tgaggttgca ccacttgcat ttatgagagg tagaacactt
    17401 gataattcat ttataatatt ggatgaagct caaaatacta cttctgaaca aatgaaaatg
    17461 tttttaacta gacttggatt tggctctaaa gctgtagtta caggagatgt aactcaaaca
    17521 gatttaccac aaaataagaa gagtggtcta atacaagcta cagagattct taaaggtgtt
    17581 ccaggtattg ggtctataat gctaacagat agagatgttg taagacatga gttggtacaa
    17641 agaataataa gagcttatga gaagcacgac aaaagagaag aatttaaaaa agaagaacgt
    17701 aaaaaagcga aaatgcagga aaagactttc aaaagaaagt aaaagtgtag taatatagat
    17761 atatagcatt attaatgaat aaaaagtagg tgtagtgaat ggatttgata ctagatgata
    17821 gacaagataa attagaagta agtgaggagt taattgaaaa gataaaggat ataatcatag
    17881 aatgtttaga ttatgaagga tatgatgata attatgaagt gagtctttct tttgtagata
    17941 ataaagagat tcatgaatta aatagagaat atagaggagt tgatagagtg actgatgttt
    18001 tatcatttcc tcttttaagt gatgattttg aggatgttga gttagaagaa gaatccctag
    18061 gtgacatagt agtatctctt gaaagagcct tagaacagag tatagagtat aaccatagct
    18121 ttgaaagaga agtatgtttt ttaatatgtc atagtatgtt tcatctttta ggttatgacc
    18181 acgatacaga tgaaaataca aaagaaatga gagaaaaaga agaacacata ttaaataagt
    18241 taaacataac aagggagtag tttttatgaa accagaaaaa actcgtcaag gtataattaa
    18301 agcatttaat gcagctatag aagggatatt gtatacattt aaatttgaaa gaaatatgaa
    18361 aatacattat ttaggttcag ttgctgttct tataataagt ttgtttttta acttttcaaa
    18421 attagaaatg ataatgttac ttatgtccat atgcttagtt gtagttgctg aaatgtttaa
    18481 tacagctata gaaaaagctg ttgatttagt aactgatgaa tatcatgttc ttgctaagat
    18541 agcaaaagat gttgcagctg gaggcgttct agtggcagca ctaaattcag tagtagttgg
    18601 atatattttg ttttatgata aattgacaga tatatctgga atattaattt ataaaataag
    18661 agaatcagaa ttacatataa ccttgatatg tatattattg gttcttatag ctgttgtagt
    18721 tgtaaaagca ttgacttcta caggtactcc tctaaaagga ggaatgccaa gtggtcatgc
    18781 tgccttagca tttgctatag caactgcaat tacacttatg actgaaagag ttgttgcctc
    18841 tactcttgca tatataatgg ctgtattagt tgcccaaagt agaattgaag gaaaaataca
    18901 taccttttgg gagactattg ctggagcttt gttaggtgtg ttgatagcta tattagtatt
    18961 tcagcttgga atgttctata attaaaataa atgtacataa aatatttaaa ataaaacaaa
    19021 tctgtgctaa ttaaattttt atgatataat gtagagtatt aaataaaaat aacttttaat
    19081 tattgtatac aggaggagtt gttgtggata acatagaatt gttaaggttg gcagaagatg
    19141 ccagacaaca ttcttatgct ccttactcag gttttagagt aggtgcagct cttttaacta
    19201 agagtggtaa agtttatact ggatgtaata tagaatgtgc atcattaggt ggaacaaatt
    19261 gtgcagaaag aacagctatt tttaaagcaa tatcagaggg tgataaggat atctataaaa
    19321 tagctatagc aagtgataac tcagaaaata atgagcaaac atatccatgt ggaatatgca
    19381 ggcaggtaat tatagagttt ggttctgata taaaaataat aacgggatat actaaaggag
    19441 aggtgtttga gcatagtata aaggatttgt tgccaaacta tttctctgga aatgatttaa
    19501 aataaattta aaagtataaa taaatttaag taattaaaac aagaagataa aaagttagga
    19561 gacagttaat gttcaaatca ggttttgtca gtatagtggg tagaccaaat gtaggaaaat
    19621 ctactttaat gaataatgta gttggagaaa aaatagctat aatgagtgat aagcctcaaa
    19681 ctacaagaaa tacaatacaa gcagtatata cagatgaaga aatgcagata gtattcttag
    19741 atacaccagg tattcacaaa ccaaaaaata aattaggtga atttatggta aaggcagcaa
    19801 cagaagcttt taaaaatgta gatttaatat tatttgtagt tgatgattct aaaaaaatag
    19861 gtcctggaga tagaaagata atagaggatt taagaagtgt taagacacca ataattttag
    19921 ttgtaaacaa aatagaccaa ctagaccaaa aagatgagct atttgatata atcaaaatgt
    19981 atgatagaga aggcattttt aaagaaatag taccaatatc agcattaaaa ggaaagaata
    20041 cggatacctt aataaaagtt atacaaaatt atttagaaga agggccaaag tattttccag
    20101 attatatgat tacagaccag ccagaaagag tattaatagc agaacttata agagaaaaag
    20161 ttcttcacta tcttaatgat gaaatacctc atggtgtagc tgttgaaatt gaaaaaatga
    20221 aagcgagaaa tgataaggaa atagtagatg tatctgctgt aatatactgt gaaagagatt
    20281 cacataaggg aataataata ggaaaaaatg gtaggaaact aaaaggaata ggtaagtcag
    20341 caagacaaga tatagaattg cttttaggtt cacaaataaa tcttcaatta tgggttaaag
    20401 tcaaagaaaa ttggagaaat ttacaaaatt atataaataa ttttggatat aatgataaat
    20461 aaaggtgggt tatatggata ggaaactgga tgcttcccaa gatttattat atgaagtaaa
    20521 atcattaatt gataataata aagtattgga gttaagagaa ttaatagagg aatatcatat
    20581 aattgatata ttcgatataa tggaaaattt agaggaagac atgaaaattc aattatttga
    20641 agtacttcct ttagatatgg catcttctat attagaagaa ggtagtgtag agttttttat
    20701 tagtatttta tctaaactgg atgtagaaca ctcgaaaaat atacttgaac ttatgtctct
    20761 tggagatatg gcagacaagt tgagtgaact tgaagaagaa gaaagagaac acataataaa
    20821 tcttttgaat caagagaatg ccgattatgt aaaagagtta ctattctatg atgaagattc
    20881 tgctggtgga acaatgacca ctggatatat ttctataaat aaagatatga cagctcttga
    20941 ggctatagac catatgagag aagaagctga agaagctgaa actatttatt atatatatgt
    21001 ggttgatgat gaagaaaaac tagttggagt attgtcatta agagaattaa taatagctag
    21061 agacgcaaat atagtagaag atttgatgag tgaaaatata atctctgttt atgtagatga
    21121 agatagagaa gaagcagtaa gattagtatc taaatacaat ttaatagcaa ttccagtagt
    21181 agacagacaa gaaaaattaa aaggaataat aacagtagat gatataattg atgtaatgga
    21241 agaagaagca actgaagata tgtataaatt tgcaggaagt tcagaacatg aaagagaagt
    21301 tgctgaaaaa gaaaacccta cattgagaga gcaaataata tcagcactta gaggacgatt
    21361 accttggtta attattacct tagttggagg gttgttggct tcgttaatac tttctaactt
    21421 agactatata atgaatcctg tatatgctcc attagtattt tttatacctg tagtaattgg
    21481 tatgggtgga aatattggaa ctcaatcatc atctgttaca gtaataacat tatctaataa
    21541 ggatttaaat tttagcaatg tggttagaga aggtattgta ggtattatta caggtctttt
    21601 gtgtagtata ataacaggta ttgtaattta ctttgttatg agaaagttag acattgtatt
    21661 aattgtttca atatcattat ttataaatat ggttttaggt gcaacaatag gtgcgtttat
    21721 gcctgtttta ttaaaaaaaa tggatgcaga cccatctaca gtttcatcac caattatatc
    21781 tacagctctt gatataactg gtatagcagt ttactttata ataacaacgg cattattgtc
    21841 aaaaattgtt taataacttt atgtatgatt tatcctcttc ttactaaaaa tataagtata
    21901 aatgtaagaa gggggttttt taaaaatgaa caatatcagt tgggaagttt ttaaaaaaac
    21961 aggtagtata gatgcatatt tatatttctg tgattgcaaa aacatacatg aagaaattga
    22021 agaagtaagg gagaaaaaag aagacgatga taatcctgaa cacccagggg atagtactta
    22081 aagctataag gtataaggaa agtgatataa tacttacact atttacaaga aagcttggaa
    22141 aagtatctgc aatagcaaaa ggagctaaaa aaaataagag ctctttgctt tcatcatcac
    22201 agttattttc ttatagcaat tttaccttaa aaaaacaagg taatatgtac aaggttactc
    22261 aaagtgaaat aataaaaagt ttttataata tatcctatga tatagaggca ttttcttatg
    22321 ctacatatat tacaaaatta gttgaaaatt ctatacttga aaatcagact aataataggc
    22381 tatttatatt acttgcgcag actctgtatt tgtatacaca ggataataca gacaataggt
    22441 ttataacagc agcattcgag ttaaaatttt tagactatat aggatttaaa cctatagtaa
    22501 ataaatgcat taattgtgaa tgtaaaattt tacaaaattc tgtgtttaat atctacgaag
    22561 gtggtatact atgtagtaag tgtagtaaac tttttgataa taatataaaa ttagacttaa
    22621 caactataag tcttatggaa tatgtattaa gaaatgatat tttaacatgt agtaaagcta
    22681 aagtatcaaa atatataaca catgaacttg agaatattct agacaagtat ttgaaagttt
    22741 atgttgataa tattaatttt aaatcattac acgtattgca aagtgtaaaa aataataagg
    22801 gagtggataa tgatgagtag taatataact attgaaatgg ttgattcagt ctttgaaaga
    22861 gttccaggag caacttatgc agaagcaaaa gaagcattag taatgtgcaa tggagatgta
    22921 attgaggcag taatatattt agaaaataaa aaaaatactt gtaattgtaa agcaaaatct
    22981 gctaaagaaa ctatggaaga agtatttggc aaagatggag aaaatataaa gtttcaatta
    23041 aaagagcttc ttagaaaaag tagtgtagtg agaataatag ttgaaaaaga tggaaaaact
    23101 atgatgaata ttcctttaac agttggagtt gttggattag cttttgtacc attagctact
    23161 ttagttggat tatcagctgc tgttataagt aaatatcgta taaaagttca aaatgaagaa
    23221 gatggagaaa ttgtagattt aggtgaattg aatgaagaaa aacttcacgt tttgaagaat
    23281 atgataacta atgcagcaaa agatgtgaag gatgtagtag ttgacaaaaa agaagatgac
    23341 aaagatgtaa ctgctgacct tatgaaagaa gaatctgaaa agaataaaga agacgaagac
    23401 gaaaataaat agatataaag tgacttttaa aaaatgaaga gaagataatt ataagaatta
    23461 tcactaacat tgacaaattt tatagaattt gatattctaa aagttgtaga agaactatta
    23521 aaatagcctt tcgtgtagac gagaggcttt attgttatag gagggatttt tatgaatttt
    23581 caagagatga tactggcgtt gcaaaaatac tggtcaaagc aaggttgtat catgatgcaa
    23641 ccttatgata ttgaaaaagg tgcgggaact atgaatccta atactttttt aagatcatta
    23701 ggacctgagc cttggcaagt atgctatgta gagccttcaa gaagaccagc agatggaaga
    23761 tatggtgaaa acccaaacag actatatcaa catcatcaat tccaagttat actaaaacct
    23821 tctccagata atatacaaga attatatttg gaaagtttga aagagattgg aattgaccca
    23881 agtgagcatg acataagatt tgttgaagat aactgggaag ctgctacagt tggtgcttgg
    23941 ggacttggat gggaagtttg gctagatggt atggaaataa ctcaatttac gtattttcaa
    24001 caagtaggta atattgaatg tgagcttgag acaggagaaa ttacatatgg attagaaaga
    24061 ttagctatgt acattcaaga agttgatagt gtttatgatt taaaatggaa tgataaaata
    24121 acttatggtg aagtatttaa taaagctgag tatgaaaact caatgtacgc atttgaatta
    24181 tgtgatgcag atatgttgtt taatctgttt gatatatatg aaaaagaagc attaagactt
    24241 atggaaaatg gccttgtaat accgtcatat gactatgtgt taaaatgctc tcatgcattt
    24301 aatacattgg atgcaagagg tgcaatagga gttagtcaaa gagcttcatt tataggtaga
    24361 gttagaaata tggcaaagac tgttgcagaa acttttgtaa aacaaagaga agaaatggga
    24421 ttccctcttt taaaggacgg tgacaaataa tgaaaaatta tcttttattt gaaatcggtg
    24481 ttgaagaaat gccatcaaga tttgtaggca gtacactaga acaattaaaa aacaatctta
    24541 gtaaattatt tgatgaaaat agaatagctt ttgataaaat aaatgcttat gggacaccaa
    24601 gaagacttac tttagtagtc gaaggattga gtgatagaca aagtgattta gaagaggaaa
    24661 taaagggtcc tgctaaaaaa attgctgttg atgcagaaaa taatttaaca aaaccagcat
    24721 taggatttat aaaaagcaaa ggattaagcg aatctgacat atattttaaa caagttggaa
    24781 aagaagaata tatatttgcg actataaagc aagatggaaa agaaacttca gaagtactaa
    24841 aagacatatt accagagact ataaaatctg tagtattccc aaaagctatg cgttggggag
    24901 gaaaaaatct taaatttgca agacctataa gatggatagt tgcattgtta aatgataaag
    24961 tattagagtt tgatttagaa ggaataatat cttctaatgt aacaaaaggt catagatttt
    25021 taggtgaaag tacaatagaa gttgattcta tagaagatta ttttgaaaaa ttagaaaaaa
    25081 attatatagt tttagaccaa aacaagagaa aagaaatgat aagaaaacaa tgtatagaag
    25141 tagcaaattc tttaggtgga gaagttgaat ttgatgaaga tttacttgaa gaggtaactt
    25201 atttagttga atatccaact gctttttatg gagaatttga tgtggattat ataaaactac
    25261 caaaagaagt tgttataact cctatgaaac aacatcaaag atattttcca gtacttaaag
    25321 aaggaaaact gttacctaac tttatagctg ttagaaatgg tgattcttac agaatagaca
    25381 atgttaaagc tggaaatgaa aaagttttag aagcaagact tgcagatgct ttatttttct
    25441 atagagaaga tactaagaga aacttagaaa gctacataga aaaattaaaa actgttgtat
    25501 tccaagtaaa attaggaact atttacgaca aaactttaag acttgaaaaa ttaagtgctg
    25561 atatacttga tagtttaggt ttagtagatg aaaaggcaga tactataagg gctgctaaat
    25621 tatctaaggc agatttagtt actggaatgg taggagaatt tgatgaatta caaggtttta
    25681 tgggaaaaga atacgcaaaa gttggaggag aaaatgatgt tgtaagtgaa gctatttttg
    25741 aacattatct tccaagattt gcaggagata tacttcctaa gactaatgca ggggttgcct
    25801 tatcaatagc tgataagttg gattcaatag caggtttctt tgctatagga atacaaccaa
    25861 caggttctca agacccatat gctttaagaa gacaagcttt aggtatactt agtatactta
    25921 tggacagaaa agttgctgtg gatataaaaa tacttattga acatgcttta gacaattatt
    25981 ctgaattaga ttttaataaa gaagaagttg ttgagcaaat aatgaatttc ttcaatgaga
    26041 gagtaaaaaa tctatttaaa gatttaggaa taagatatga tgttgttgat gctgttttaa
    26101 gttctgatat aaatgatgtt tctgatatgc atatgagagc tttagagctt aataattggc
    26161 tagaaaaaga tgaattagtg gagatgctta ctgcatttaa tagagtttct actctagctc
    26221 aaaaagcaga aagtgataaa attgatgaat ctttattaaa agaagatgct gaaattaaac
    26281 tttacaatga atttaaacaa gtaaaggtaa aagttaatga attattaaag gataagaaat
    26341 atggtattgc tttagatgaa tttgcatctt taagaccagt agttgataat ttatttgata
    26401 cagttatggt catggataat gatgaagcta ttaagaacaa tagacttgct ttacttaaac
    26461 aagtatatga tactatgcta ggaatttgtg atttatctaa aattgtttat aaataaaatt
    26521 taaactattt ataagaaata aggctgtctc aaaatagaga tagtcttatt tttcattgtt
    26581 gagatgatga aaatatattt gatttcatag aaaaagagtg tctcataaac taaaaagttc
    26641 attttgagac acccatgttt tttgtaagta aatataataa attatatttt atacttatca
    26701 gttattttgt ttaaaatatt atcatttgaa tttacccatt cataaaaatt gcctgaagat
    26761 gcgtccgata ttttataaag catttcagga atatcactag ctttaaaatc tccaagtgat
    26821 tttcccaatc tagtcttacc tatttcaaaa ctgcctccaa caaataattc aaatgcatct
    26881 gtagatatac catcaacatt cttcttttta ccacataaac ctatgctacc aacttgatga
    26941 acaccacatg aatttaaaca tcctgatata tataacttag gtgctttatt taaaatttct
    27001 tcattatttt gtaatctaaa ataatctatg atttcatgta acattttctg actattttgt
    27061 atgcccattt gacaaattgg aacacctatg caagatatac tttgttctaa ttgagaatta
    27121 caactaatag ttttacttat ttctaatatt ctttttgctt catttccatt taaatttaaa
    27181 atatataatc cctctgtcat tcctagtcta atcataggat tttcaacatt atctaattct
    27241 ttaagtaaag tacttaaatc ctttgttaac aaaatacctc caactggatg tatatataca
    27301 gtgtataaac catcctgttt ttgaggtatt agtctagaat cttgtatatc tattttaact
    27361 ccaggtttag agtaatctat ttcttcaata ttgagttcaa gactaccatt ttctttttct
    27421 aatttatagt attctttaaa tttttctaag aatagttctt caccaagtga ttcgaccata
    27481 tatctaaccc tagctctgtt tttattttca taatttccat aatccataaa gaatttaatc
    27541 ataccttcaa cacaatataa ggcgtctttc ggctttataa gttcatcaag ttctaaagca
    27601 actttgggtt gtttacctag acctccacct aagaaaactc tgaaatatgg ttcatcattt
    27661 ttcaaagtag caacaaatcc taagtcttgt atactacaat gagcagtatc tgtgtaacaa
    27721 ttagaataag aaacttttaa ttttctaggt aaatggtatg tagtagcatt tttgataaaa
    27781 tatttatcag tagcaactgc atatggagta acatcaaaga cttcactagg gtcaactcca
    27841 gataatggag ataatccgac atttctagga taattaccac caccacctct agtgaatata
    27901 tcatttttta ttccttcttc catgatatca acaattgaat ttatagataa atcgtgtaat
    27961 tgtatagctt gtctagttgt taagtgtatc ttatctaagt tgtgtttaat agctatttca
    28021 tagattttat taagttgata ctgagataaa actccagcag atactctaag tcttatcata
    28081 aaagattttt tgtctctttg agcataaaca ccatatccac cagaaaatcc cttatattgc
    28141 attttattta tttctccatt taaaaatttt gaagaatatt ctttaaaatt aggtagttca
    28201 tttaacaata tctttttaaa attatccatt atttcacctc attgttagtc ttgtataaaa
    28261 atatatctat tgtataatag atatatatat tattatctag tcaggataaa aatataacag
    28321 aataattcta ttaaaagtaa gaataataag gacatagtga gtattttgtt tttattttac
    28381 aaattaagta taatattttt gttttataaa atataattaa gataaataga taattttgta
    28441 attttaatat ataatattta gtagagtgtg ggagtagcac ttaatatttg caggaggagt
    28501 ttttgtatat gggagaaata ataaatgcat tagatagagc cctagacata attttgctgt
    28561 tatatcatga aaagagagaa atgggaataa ctgaaataag taaggctatg ggagtatata
    28621 aaagtactgt acatagaact ttggttactc ttgaaaacaa aggttttgtt atacaaaatg
    28681 cagagaatgg taagtattgg ctaggaataa atttatatgc aataggtatg gttgtgggag
    28741 aaaaaatgtc attgactgaa atagtaaaac catatacgaa aaaattaaat caagaattta
    28801 acgaagtagt taatgtatct atattagaag aaagagctca agattctcca agaagtatca
    28861 taatacataa agagtatggt tcaaatcaat tactttcagt aaatccatcc gttggttcaa
    28921 gtagtgaatg ttattgttca gcagttggta aatgtcttat ggcatttaat gattctatag
    28981 attttgaaaa atatagaaaa acaccaatac acaagtatac agaacataca attgataatt
    29041 gggatgatat gatgcttttt ttagcaaaaa taaaagaaca aggatatgct atagatgatg
    29101 aagaactaga acatggacta acttgtattg gagcacctat cttagataaa aataataaag
    29161 ctattgcagc cataagtttg tcaggtccaa ctataagaat gagagaaggg gactttgagt
    29221 ataagattaa aagagttata gaaactgcaa aaagtatatc tgaacttttt agatgatttt
    29281 taatctcgat aataaaaata ggctatattt aaattgttat ttgtaaatat cagtttaaat
    29341 gtagcctatt tttctttaat ctaaaattaa atattcttaa aatttaaaat gttattgaaa
    29401 tatataagcg aatgtgttat cttttaatta agcaataaaa atagaacaag gttctatact
    29461 atagaacaaa attgatgcat atatagtatt tatacaaaat ataaatatta tatatttaaa
    29521 attaagtaaa aatattacat aaaggggaga tttgagaaat ggaaaaagtc aagttgagtg
    29581 ttgaaagatg taagggatgc tatctttgta ttgaagcttg caaaaaagat gcaataagtc
    29641 tttctgacta cacaaataaa aaaggctttg taactattaa agtcgatgaa gaaaaatgta
    29701 ttcaatgtgg cgcttgttat acaatgtgcc ctgatttagt atttgagata ttataaatta
    29761 tttttattag aggggagaaa aggtatgcaa agtttattaa ttaaaggaaa tgaagcttta
    29821 gcagaaggtg ctatacgtgg tggttgcaga ttttatgcag gttatccaat aacgccacaa
    29881 tcagaaatat tagaaatgat ggcatggaga caaaaagaag ttggaggagt atttatccaa
    29941 ggtgaatcag aaatagccag tgtaaatatg gttatgggag caagtgcact tggagcaaga
    30001 gctatgactt cttcatctgg tccaggattt tcattaaaac aagaaggtat tgcatattgg
    30061 gtatcagctg gtttgccagc agttgtagca tgtgtgcaaa gatatggtat aggtgatgga
    30121 tttataggag ctggtcaaga taattattgg caagcagtaa aaggcggtgg aaatggagat
    30181 tatcatttaa tcgtgttagc acctaatagt gttcaagaaa atatggatat ggcatatgaa
    30241 tcttttgaat tggctgaaaa atatatgcat ccagtactta tactttctga tgctacaata
    30301 ggtcaaatgg tagaaccttg tatagcacca ccaatgaaag aatacgatat ggataaagct
    30361 ccatgggctg caaaaggaac acctgtagga gaaaaaaata aagtaataac tgatataaca
    30421 tattttgaag accaagatac atggcaaaaa gggtatatag aaaaaatgag aaaagttcaa
    30481 gaagatgaac aaagatggga agagatagaa atagaagatg ctgaagtggt atttgtggca
    30541 tacggtattt catcaagaat agcagaaggt gcagttagag atgctagagc aaaaggaatg
    30601 aaattaggtc taataagacc aaaaacattg tggccttacc caagaaaagc atttaagaaa
    30661 ttaagtgatt cattgaaggg acttgtaaca gttgaaatga acatgatggc acagatgaag
    30721 gaagatgtaa ttatagcatg tgataataga tttccagtat attcagtagc aacagctcaa
    30781 tatatggcag aagtaaatca attaatagat tttgctcaac agattataga tggaaaagct
    30841 aagcaagagg aggtatttta attatggcta aaaaagcacc agcaataatg atgtgtgaaa
    30901 atatgttctg tggaggctgt ggacactcta tagtcgatag aattattgga gaagtattaa
    30961 cagaaatgga tatatgtcaa gatacaatta tatgttcaga tataggttgt aatcatatgt
    31021 ttcaatttag tatgaatgta gatgttgtcg ttccacctca tggtaaaatg ggagcagcaa
    31081 tggcagctat gaaaagagtt agaccagata aatatatatt tacaatagct ggagatggtg
    31141 gagcttatgc aatagggctt ggagaaacta tgtcagctgc tactagaaat gataatgtaa
    31201 tatttcttgt tatgaacaat aatatgtttg caatgacagg agggcaaatg gctccaacta
    31261 ctatagaagg tcaatataca gcttctactc cagcgggtag aaattacgca gatcatggtt
    31321 caacatttga tgttgtaaaa gttatgggaa atttagatat agcatatcta gcaagaggaa
    31381 caataactaa ccctaaagaa ataaatacaa caaaaagata catcaaaaaa gctctagaaa
    31441 aacaaaaaga aaagaaaggt ttttgcttaa tagaagtgct tgtaccatgt ccaacaaact
    31501 ggggattaaa gccagtagat gctatgaaga gaattgaaaa tgaacttgtt ccaaagtatc
    31561 cacttggaga atttattgat tagggaggaa ataatatgtt gaatgaaatt atatgtgctg
    31621 gttttggagg acagggagtt cttacagcag gaaaaattat aatgtatgtt gcatataagg
    31681 cagaaaagaa aattacatgg ttcccatctt atggaaatga gatgagggga ggaagtgcaa
    31741 attgcaatgt tgttgtaagt gatgaaaaaa ttgcaaatcc ttgtgtaaaa aatccagata
    31801 tactattggg aatgaacagc gtatcagtag ataagtatga aagtttaatg aaaaaaaatg
    31861 gttatatgtt tgtcaatgaa tcactagtaa gtaaggatag aaagtacaga gaagacatta
    31921 atgttataaa agtacctgtt actgaaatag cacaaaattt aggcaatgaa aaagctgcta
    31981 acatttgcat gttaggtgct atggtaaaga gtttggattt atttacaaaa gaagaatttg
    32041 aaagtggtat gtgtgaatac tttgaaaatc aaggcaaagg taaattcaat tctaaaaatg
    32101 tagaagcatt tgaagctgga tacgactacg aatgaatatt ttaaatattt aaaataatta
    32161 ttgaaatact tatttttata tgttagtatt taattaataa aatatagaac gtcgttctga
    32221 acaataggac gaagtcttta ggtcggaata caagaacgac gttctgtata gtagaactag
    32281 ggggtgttaa attgaaagtt ttagtaataa atcctggtgg tacatccaca aaaatttctg
    32341 ttttccaaga tgaaaatgaa attttgaaaa aaaatataac ccatacaaga gaagatttaa
    32401 aaaatttttc taaagtattt gatgaatatg attatagaaa acaactgata gtagatattt
    32461 taagtagtga gaatcatagt ataaattctt ttgacgcagt agttggtcgt ggtggactta
    32521 tgaaggctat aaaaggtggt acctatactg tgagtgaaga aatgattgag gatatgagaa
    32581 atgaaataaa tggggaacat gcatcaaatt taggtgcatt attagctaaa actatagctg
    32641 atgaaatagg agtacaatca tttgtagtag acccagtttc tgtagatgaa tttgatgatg
    32701 tatcgaggat tacaggaata tcagacatag aaaaagcaag ttggttacat gcattaaacc
    32761 ataaagctgt ttcaagaaaa attgcacaaa aactaggggg aaaatacgag gattataact
    32821 ttattgtagc tcatttaggt tcaggaatat cgattgtagc acataaacaa ggcaaaatga
    32881 ttgatggaag tggtggtagg tctgatggtc cattttctcc agaaaggagt ggtggattac
    32941 ttgcatatcc tcttatagaa ttgtgttact ccggaaaata tactcatgat gaaatggtcg
    33001 caaaagtaag ttcgattggg ggaatgtacg attatttagg aacaaaggat atgatagaaa
    33061 ttgaaaacag aatcaaggag ggtgatgaaa aggcaaaact tataatggat gcatttattt
    33121 atcaagtagc aaaagaaatt tctatgtatg gagcttcact ttcagggaat gtagatagag
    33181 ttatcttgac aggagggata tctcactcag aaatggtagt caaaggagta gcagaaaaag
    33241 tttcatatct agctccaata gagatagtac caggagagat ggaaatggag gcacttgctc
    33301 taggggcact tagagtactt aatggagaag aaatagcaaa aacttattaa tatttaggag
    33361 aaagagtatg ttaaaagatt ttaataattt attagaagag gctaaaaagc ttgaacctgt
    33421 tattgtaagt gttgctgttg cagaagacaa agaagtatta aaggctgtga aatcagcaac
    33481 agatttaaac attataaagc caattcttgt aggagacaag gataaaatag aaagtatatc
    33541 aagagaaatt ggattaaaag gtcatgaaat aataggttgt caaaatcccg atgaatcagt
    33601 taaaaaagct gtagagatag ttaaaaataa ggaagctaaa gttcttatga agggattagt
    33661 aaatacaagt gtctatatga gagctatatt aaataaagaa acgggtctta gaacaggaag
    33721 attattaagt ttgcttgcag tatatgaatt acctcaatat cataaacttc tttattgtac
    33781 tgatagtgga ataaatgtat cgccaaactt aaatcaaaaa aaagatataa tgacaaatgc
    33841 acttcttact atgaaaagta taggcatgga aaatcctaaa actgcgatac ttactgcaaa
    33901 tgaaatggta gaccctaaga tacaatctac agtggatgcc aacctacttg tagagatgga
    33961 aaaatcggga gaaataccaa agtgtatagt ggaaggtcca atagcatttg atgtagcatt
    34021 tgatgcacat gcagcagaac ataaaggtat taaaagtaat attgctggtg atgttgattt
    34081 attagtattt ccaaatatag aagcagggaa tatattagga aagtcatggc ttagatttaa
    34141 taaagctaag tgggcaggta tagttttagg agcaacaaat ccgataattt taggttcacg
    34201 ttctgacaca gctgaaataa aaataaattc aatagcatta gcgtgcctag catcacaata
    34261 agaaattatt attaaggagg aaaaagtatt atgaaatatg caaaaagaat ggatatggta
    34321 aaagcttcag ctataagaga ttctcaaaag aaaatagctc agaaggtagc aagtggtgga
    34381 actgttatat cttttgctgc agggcttcct gacccaaatt tatttccaat agaagagata
    34441 agtgaaataa ctcaaaaagt acttaatgaa aggggaaaat ttgccctagc atatggacct
    34501 actaaaggtg aagatgagtt gttagatcta ttagtaaaaa gaatgaaaga agaagaaaat
    34561 attgatacaa aagcagaaaa tattataata actactgggt cacaacaagg gatagcatta
    34621 agtgctatga ttttattaga aaagggagat gtagtagtta cagaaaatcc aagttactta
    34681 ggtgcaataa atgcatttag accttatgaa tgtgattttt taggagtaga tacagatgaa
    34741 gaaggaatgg taacagatga tttagacaaa ttattatctg aaaattctaa cataaaggtg
    34801 atatatgtaa taccaacatt tcaaaaccct acaggaaaaa catggtcttt acaaagaaga
    34861 aaagatttta tggaagtcgt gaataaatat gatgtcatag taatagagga taatccttat
    34921 ggagaaataa gatttacacc agaacctcta cctacactta aatctcttga tactaaagat
    34981 aaagtattgt accttgggtc attttcaaag gtattatgtc caggatttag agttgcttgg
    35041 atgtgtggta atgtagacat catagataag gcagaacttt taaaacaagg tatagattta
    35101 cagtcaaatc aattttcgca agtacaagta atagaatttt taaagaaata caacttaaat
    35161 gaacatatag aaaaaataag agcagagtat aagaaaagat gtctgctaat gttagatgct
    35221 atgaagaaac atttcccaga ggaagctaaa tatacagaac cagatggagg aatgtttata
    35281 tgggttgaac ttcctgaaag tataaatgta gatgatttat tagataaagc cattgatgct
    35341 ggagttgctt atgtttctgg agagtccttc tttgcaaata atggtccaaa aaatactatg
    35401 agacttaact atacaacaat gtcagaagaa caaatcgtta aaggtgttga gatacttgca
    35461 gaagttataa aaaaagaact caattatgct tgtgaataaa tataaaaagt gagggtttaa
    35521 atatgggaga aaaagaaaac ggtataaagt ttggaaagtt aatgtttcaa cttgttatta
    35581 tagcaatatc ttgggaatta gtttatatta taccatttat ccaatataca ttatatgatc
    35641 caattctaaa agcattagaa tgtacaaaca ctcaactagg ttttctactt acaatttatg
    35701 ggttaggtaa tatatttgga gctcctatag gaggctggtt agcagataga tttgattata
    35761 gaaagataat tttaggttct gtatttctta atggagtagt atcattttta tttgcattta
    35821 atatgaacta tccatttgca gttgctactt gggtaggttg tgcaatcaca tcattagtta
    35881 tgaactatcc ttctatggta aaaatactta gagttatagg aaaagataat caaggtaagg
    35941 tttatggatt taatgaagct atggttggag tttctggagt agtaatggga gcaatattct
    36001 tatatattta tacatgcttt gcaactccta cattgggtat gagatgggta atgatatctt
    36061 taggaatatt atcaatagca atgtgtccag tattatggtt tgtaattaaa gatgtagata
    36121 ttaaagaaga aagagaagaa gcaggtgaaa aaatgtctgc tggtgatttt ataacagtac
    36181 taaaatcacc aaatacttgg ctagtaggaa taagtatatt ctgtgtatat tcattcacag
    36241 ttacaatgtc ttattttaca ccatatataa catctgtact tggaggttct gtagcattat
    36301 ctggtgcatt agcaataata agacagcatg gtctaaaatt atttggagca ccatttggag
    36361 gatattgtgc agacaaatta aaatctccta caaaagtact tttaccaata tatgtatttg
    36421 gtatagcagt aatagtttta ttcctagtat tacctgcatc aactccaatg actatattca
    36481 tagctttaac atttgtagta ggtatattag gttacatggg aaaaggtata tattatgcag
    36541 tacaagatga agtaaaagtt ccagttaaat attcagcaac tactataggt atagctgcag
    36601 ctttaggatt ttcaccagat gtattccaat ttgcattaat aggacactgg atagatactt
    36661 atggcaataa aggttacaca tatacattta tattccaaat agcaattcta gttattggaa
    36721 tactatcttg tttatatata ttaaaggtta aaaagagaag agcaagtaaa ttagaagctt
    36781 aaatattcaa tttggtaatt aagtatatat aaatacaata cttattttga tgaatgtgat
    36841 tgtataaata acataaaaaa tatcaataga ttaagtttta taataatgtt tagagaataa
    36901 agtataaaaa tcaaaatgtt atcaagtttt ctaggtttta acataaagtt acccctgaag
    36961 gtgtaaaatt gagtggcaat tattatctat tttcttacct acataaacct atttaattta
    37021 aattctaaga gattttaaaa atagatggta gttgtactca attttaaacc tcataataaa
    37081 tacgaaaatt taaaatctaa ctagatacta tttaattata tattaataaa tatatattgt
    37141 actatttata taataataat ttcaggcttt tcattatatg tattccaatt tatattgttt
    37201 gattattatt caaacacacg tcgttaaaaa ttttataaat ttatgtttag atatgattaa
    37261 tactcttgat tttagaaata aatatttgta gattgtagat taaagataat ataaataaat
    37321 agaaggtaga ttttttagat atattaatat aaagattata taaaatattg aaatatagct
    37381 gtttaaatta ttgtctataa atataaaatg aatggtggtg agttaagtga ccgatttagt
    37441 taacaccatt aatggaattg tttggtcacc cgtattagtt gcaatgtgtt tgtgtatagg
    37501 attatatttt tcattgaaat taaaattttt tcaaataaga gatgtaaaag aaatgtttac
    37561 tctcttgctt gaaggagata gttctcatgt tggtatatca tcatttcaag gttttgctac
    37621 atctatggct ggaagaatag ggactggaaa tatagctggt gtagctgtag caatagcaat
    37681 gggaggtcca ggagcattag tatggatgtg tataatggct acattaggtt cagcaactgc
    37741 atttgtagaa tctactctag ctcaaatata taaagatgag catgatgggc agtatagagg
    37801 aggacctcca tattattttg aaaaaggtct tgggtggaaa aagtgttcag ttttattttc
    37861 aatagttgca ataataagtt atacattttt tatgcctggt acacaatcaa acactttata
    37921 tctagcaatg aatcaagcat ttggattttc aaaaatgaca atagcagtag catcttctat
    37981 tgcattagcg ataataattt ttggaggaat taaaaggatt ggtttatttg ctgaaaaagt
    38041 agtacctata atgggtggag cataccttat tatgacatta gtaataatta taacaaatat
    38101 tagtttagtt ccagaggcta ttgtaacaat gtttaaatct gcatttggat ttggttcagt
    38161 ttttggagga atgctaggtt cagcaatatc ttggggagta aaaagaggta tatactcaaa
    38221 tgaagcagga gaaggttctg ggacttatgg tgcggcagca gcagaagtga gtcatccagc
    38281 caaacaagga ttagttcaag cattttctgt ttatgtagac acattattaa tatgtatggc
    38341 tacaggtatt atgattgtaa taacaggaat gtataatgtt acaggtgttt gcacagcact
    38401 gccaggtgta gaaccgggac cacaatatgt acagagtgca ataaatacat tacatccaca
    38461 attaggtgat atttttatag caatagtttt attattattt acatttacaa ctttattagc
    38521 cttctcatat atgatggaga ctaatgtatc ttacttagta actagaatta aagcagaaaa
    38581 acatgaaaag tacataatta atgtctgtag atttatgctt atagcatgtg cagcaatgtg
    38641 ttgttttata gaaccactta caagctgggc attaggtgat atatgtatag gtgtattaac
    38701 atatataaat ttaattgcca tctttacact aaaaaaacca gctataaagg catattatga
    38761 ttatataaga cagaagaaga tgggaataaa aaggagtgat agaactttta accctataga
    38821 gttagatata agaaatgctg agttttggga aaatagatac ttaaaagagg ataagcatga
    38881 gttaaattaa agttataata aaatataaaa attactaaat aaatttagtg ggaggtaaga
    38941 ttggtatgaa agaaagaata atacaagtgg ctgattcaaa gaaagaaaaa attctagggc
    39001 tatgtcaatc tttatatgat gaaccagaaa ttgctttgca agaatataaa tctgctaaaa
    39061 aaatatcaga atttttaaga gaagagggtt ttgatgttga ggaaaacttg gcaggtatgg
    39121 ctacagcttt taaagcaacc aagaaaaatg gagatggtcc taaaatagca tttatagcag
    39181 aatatgatgc tcttccaggc aatggacatg catgtggaca tcatttaatt gcatctatgg
    39241 gagttggagc tggaatagct ctttcaagta tactggatac ttacaaggga gaagtaagta
    39301 taattggaac acctgctgaa gaaactggag atggaaagcc ttacttgata gaacatggtg
    39361 tatttgatgg ctatgatgca gcaatgatga tacatccaaa ttcaaaaact tgtgtaacac
    39421 ctgaaattat agcaatagga ggtctagatt tcatatttac tggaaaagca tctcatgcag
    39481 gagctaagcc atataacgga ataaatgctt tagatgcagt agtattacta tataataata
    39541 taaatgcact aagacaacaa ttagtagatg gaacgagaat acatggtatt atactagaag
    39601 caggaactgc tgcaaatgta atacctgata tgggaaaagt aagattagag ataagggcaa
    39661 aagaacaaaa ttactttgat gaagtagtag aaaaagtgaa aaattgtgct agaggtgctg
    39721 caatagccac tggatgtgaa cttgaatttt atcactttga accaacttgc caaggattaa
    39781 atgaaaataa agttttggta gacatattta caaagattat ggaggaattt ggaatatatg
    39841 aagatgaaca agtaatgtta ggttcaacag atatgggaaa tttaagtcaa ataatgccat
    39901 gtatacatcc tttgatgaag ttttctgaaa atggagaaga actacataca aaagaatttt
    39961 tggaggcttc tataaattca tatgcaaaag atagagtaat tgatggtata aaaatattag
    40021 ctttaacagg atttaattta tttgaaaatc cagagttatt gaaaaaaatg aaggaagagt
    40081 agaacttaga ctatttttaa ttatactctt taaaataaga agagtgtctc aaaatgaact
    40141 ttttagttta tgagacactc ttttttgtat gaaattaaat atatttttat tattttaaca
    40201 atgaaaaaga ggcttatctg tttttatatg acctttttta acttgtagca caaaatatca
    40261 tgaatgatta caattcgaat gataaaatcg attataagat atataggagg ggtacatatg
    40321 gaaactttag aagtaagaaa tattgaattg agtggtacaa attatgagat aggatacaga
    40381 ctaggagaat tggttgctaa tatgccagag ataatagaag gtcagattaa taaatctaat
    40441 ctggtaagca aaaaagaaga aaaagagatg atagagttgt ttgataaata ttgtccaggt
    40501 cttaatgaag aattgcaagg ttttgctgat gcaattcaag tgaattgtaa tcaaatttta
    40561 tattatacta tgacttatct taaaccaggt tgtagtcaag ttgcattagc accagaatta
    40621 actgaaaatg ggcatgtact atttgcacgt aatttcgatt tctcacacaa tatggaggat
    40681 tttgttttat gtaaaactaa ggtaaatgga aaatatgcac atataggtac taccgttatg
    40741 caatttggaa gaggtgaagg tatgaatgaa tgtggcttag gtgtaagtca gtcatcatgt
    40801 ggtattcctg taggcaattc agatggttta agaaaaccag caattgtagg tttacagttt
    40861 tgggctgtta ttagatattt gttagaaaat tgcaaagatg tagatgaagc attagaatat
    40921 ttaaaagata tgccaattgc atacaatatt aatttattgc ttgcagataa gtctggaaac
    40981 attgcacttg tggagacttt agatgggaaa aaagaagtaa atataatcaa tagtttagaa
    41041 agtaaaagag aatatttttt acactctact aatcatacac atattgataa acttcataaa
    41101 ttagagccac aatcaatgaa aaattctatt catagatata aattaattaa agaatatata
    41161 aataagtcta aaaaaattgg agagaaagag ttaatgaatt tattgtcttc aaaatatcca
    41221 aatggcttat cttgtaatta ctataatgat ttttttggaa ctttaaaaag tatagttatg
    41281 gatttaaata taggaaagtt taatatttta tggggtggac tagagaataa atgggagagc
    41341 tactatttaa aaaatgacat taaatctata acacagagga taaacataaa tattgaaaaa
    41401 gcaccatctg acttttttga tttcattgaa taaaaaatgt atttaattat tctaaataat
    41461 taggatatat aaatcaacta aaagttttta tttatcgtat tgaattagtt ataatatttt
    41521 agtatagtaa aaaatagtgc tattttacat atttaaagag tttaagtaga agagaggtgc
    41581 catattggat tacttaaagt attttaaaaa agctgtaatg tatattgaga ataatttaca
    41641 tgaaaatata actgtaaatg atgtggcaaa agaaactgga tattcttatt atcatttaac
    41701 acgcttattt aagtctatgt ttggtgagag tgtaggaagt tatataaaaa aaagacgatt
    41761 agtaagtagt acaaaagaac ttttatatag tgataaaaaa gtgatagata tagctattgg
    41821 tagtggattt gaatcttcgg aagcatttag cagagcattt aaatctatat acaaggttag
    41881 ccctatagaa tatagaaaaa atagaattga tgtatttgtt ggtaaaaaga aaaaattaga
    41941 gttagatttt atgagacatc ttgttggaaa tataacgatt aaaccagtta taaaagaaat
    42001 agaaaaaata aaggtaatag gtataaagga taaagtaatt ttagaaaata attcattacc
    42061 tgacttgtgg gagaaattca gaaaagtaca tcatattgtc ccaaatactt tgccatcaaa
    42121 gagagttttt ggaatctgtg aagcaatctc tgaaatacac ctagttagtg aaagtatgga
    42181 atttaatgag ataataggac ttgaagttaa tagttatgat gtgattccaa attcatttgt
    42241 ttcaaaaact attaaggaag gaaagtatgc tatatttact catactggta gcttagataa
    42301 tttagacaaa acttatgaat atatatgggg tacttggttt ttaagttcaa aagaagaact
    42361 tgatataaga gatgattttg aagtatatga tgaacgattt ttaggtccta gtaatgtgaa
    42421 ctcacaaatt gatatttata tacctataaa ataatctata agaaaactaa aaaataattt
    42481 gattaagaaa tagatgttcg taaaagagca tctatttttt atttttatca gatttagata
    42541 attttaataa taaaagaaat aaaaataagt tcttaaattt ctaagaagaa tttagaatat
    42601 agtcgaaata agaattgtat atttctgaaa attctaatta gtacaatttt ttcctttata
    42661 aaattgaaaa aagatgtatt tatttatcaa aaatattata ttaaaatgat attaataaaa
    42721 aaacatttac aatattagca tatagtacag aaaaaagatg taatttgtaa aatttatggt
    42781 aagcagtaaa attaaaaatt taaagatatt atagggaggt tataatttta tggaaaacgt
    42841 tattacaggt ctaagtaaag gtgcagagtg gtttatcggt ttgtttcaga aagggggaga
    42901 acaatttata ggtcttgttt caggtactct tccaacgcta attgtattaa tggtagctat
    42961 taactctttg attaagataa ttggtgagga aagagttaat aattgggctt ctaagctagg
    43021 aaagaactct tttacaagat atattctact tccattagta tcagtatttt tcttgggaaa
    43081 tcctatgtgc tatacatttg gtagattttt aaaagaagag cacaaagcag gattttatga
    43141 tgcagcagtt tcatttgtac atccaattac aggtcttttt ccacatgcaa atgcaggaga
    43201 actttttgta tggttgggaa tatctgcagg tttgactact ttaggtaaag aaactactac
    43261 attggcattg tggtatttta tagttggtct aattgtaatt tttataagag gtatagtaac
    43321 agaaaaaatg tatgcttttc taacaagaaa aaatagagca acaaataagg cttaggaggt
    43381 agataaaatg gaggagaata aaatattaaa aatagaaaag ggaacttcag gctggggagg
    43441 tcctttgtat ataaaaaaag aagggaatag aaataagata ctgtcaatga ctgctggtgg
    43501 tatccatgaa gtaacattaa aaataaaaga actactgggt tgtgaaattg tagatggatt
    43561 taaaactgga gtttctgatg aagaagtagc agtagttatt attgattgtg gtggaactgc
    43621 tagatgtgga gtttatccaa agaaaaaaat acctacaata aatgtaaatc cagttggaaa
    43681 aacaggtcct ttagcaaaat ttataacaga agaatattat gtatcagatg ttaatcctaa
    43741 ctgtataagt gttgtagatg gggaagatat gccacaaaaa tctcaagaaa ataaaagcga
    43801 aaataagagt tctattagaa agcctgataa ttatgatgag gttaagtcaa aagcacaagg
    43861 tgaatatgct aaaaagaaca taatactttc tattggtcaa ggggcagggc aagtagtatc
    43921 aaaattttat gatgcaggtc gagatactat ccaaatggta atgaataacg taataccatt
    43981 tatggcattt gtaagtatgc ttatgggaat tattttagca tctggactgg gagactggat
    44041 tgcaagagtt atatctccac ttgcaggtaa tattggtgga ttattgatta tatcagtaat
    44101 ttgcacactt ccattcttat caccaatact aggacctggt gcagttatag ctcaagtagt
    44161 agggactctt gtaggtacac aaattggtct tggtgcaata ccagcatatt tagcacttcc
    44221 agctttattt gcaataaatg ggcaagctgg atgtgatttt gtaccagtag gattaagttt
    44281 gggagaagca gaaccagaaa ctgtagagta tggtgttcca gctttatttt actctcgttt
    44341 aataactggt ccaatagctg ttattattgc atatggagtt gcagtatttg cattgagata
    44401 atatttaatg tcaatataag gcttggaatt aattaataaa attgtaaata taaatttaag
    44461 ctagtcaaga ttttttgtta atgaaagact agcttttata ctattaatta gctgtaatta
    44521 tcgagttaga ttaatactaa attttccttt attaaagtga aattttatgt attaatttgg
    44581 tttcaaattt gtactagaat gaaatcaagg aggagataca aaatgagtgt aactgagaaa
    44641 attccttcta ttgaccgcaa gatatttaag atattgatta tgtgtagtaa aaaagagttt
    44701 gtgagtatta actcaatagc aaatgaattg aatgtcacaa ctagaagtgt aaggacgtat
    44761 ataaaacaat tgaataaaga tttaggtaat gatatagcag taatcaaata tataaagggt
    44821 caaggctata aactagaaat aaaagatgag cagatactca ataagattat tgatatgaat
    44881 agaaaaaatg tattttcatt aaattcaaaa gaagatagag tagagtttat actaaattac
    44941 ttgatagaac tagatggatt tattacactt gatagtttag ctgatgagat gtgtgtagga
    45001 agaactactt tagtaaatga tttccagtat gtagaaaaag ttttggcttc atataattta
    45061 aacttgatta aaaaacagaa tacaggtatg aaattaaatg gaaatgaact tgatattaga
    45121 ctgtttatat taaatcagtt gtataaaaac tcaaggaaag atttcagtaa ttcaaagtat
    45181 tttaaaggaa tcaaaaaaga agaaatcata aatttagaag aaaaactatt gactttattt
    45241 aaaaaaaata atttttatgt tacagatgaa atgttaagag aagttattaa ctacatagta
    45301 gttttagtct atagagtaaa agaagaaaag aaagtaaaag attatgatgt aaagtttgat
    45361 ttacttaaat catatgatga atactttatt gctagggaaa taaaacatat aatatcagga
    45421 atgtttgaat gtactttaaa tgatgaagaa attatttatt taactatacc gttagttagt
    45481 ggaaatgcac cagcatcaga atgtgcttta aatagtagta gaattagtaa aaacattgat
    45541 gagttgatgg aaaatatatt taatcaaata tatgtcgata tgggtatttt tataaatgag
    45601 gatgaattaa gagtaggtct aggatatcat cttagtttta ctttaaacag gctattattt
    45661 aatataaagt taaaaaatgt tttgttagaa gagattaagc aaaattatat acttccattt
    45721 aaattagcac aaatagcagg aaaagtaata gagaaaaagt ataacttaga agtttcagaa
    45781 gatgagattg gatatatagc aatccatttc agtggatatt tagaaagaaa tagcagtaga
    45841 ttctattcaa taaaaaaaat agcaattata tgtagtacag gtctaggaac agcaaaacta
    45901 ttgaaaataa gagtagaaaa actgatagga aataatccta aaattgatac tctttcaagt
    45961 tttaatttga gaaatattaa cttagatgaa tatgatatag tttttacaac tattgattta
    46021 gacacatcaa atataaatac aatagtatta aaaataaata caatatttga tgaaaacaaa
    46081 ctaagagaac aattaaaaac agtattgtgc ctaagagaag ggaatattga cactactaat
    46141 tctacaaatt tactaataaa taatttgtta gatgaagata aatttatgat attaaatgaa
    46201 aagactatac ttgcttcatt agaaaaaatg atggaccatc ttatggattt aggatgcatt
    46261 gatgataagt ttagaaaaaa tatagtcaat agagaagaaa aatctcccac agtatttgat
    46321 aaaggtttat tatttcctca ttctgtaaat gaaaaatcag ataagttttt aatggcagtt
    46381 ggaattttag aagaaccaat agaatatgca aatagaagta taaaaataat tcttatgaca
    46441 atgtttccat gtgagaataa gatagactca gacttattag taaaaattta tgaagaagtt
    46501 ttaaagatag gccaagatgt aaaattaacg aataagataa gtaaatgtag aactttttta
    46561 gaatttaaaa aggtgttact taaaaacctt atataactat tgtacagcat ttatagttgt
    46621 tatacagatt taaaaataat gtgatttttg gaaggtgtat aaatatgaaa ttattagtga
    46681 ttttatttgt tgcttatatt ttaaatacta tatttataat gaagcagaat aaattgtatt
    46741 ttaaaacttt aaatagagct aaaaaaaagg gtgatatagt gtctacaggc aaaaagaaaa
    46801 gctatttttc taaaggaagt atagccatta tatcttcaga ttcagatggg tttataaagt
    46861 ttggagaaat tttaaaagga cgtacagtaa tggctaaatt taaagagatt gaagaaatag
    46921 aaggattaga tatatatagt gcagagcaaa aatttaaaga tgaagagagt attgtacaag
    46981 ctataaaatt tataaaagaa aaaataaatg tttcttttaa ttaactcaat ataaatataa
    47041 attatcagga ggaatgtcat gaaatatgaa gttaagataa gtgggatagg cagtttagtt
    47101 aaagagttaa tggatgaaag caattgtctt attatatatg atgaaaccat taatgatgac
    47161 gacttaaagg atatatctgt aattcattca atagcaacgt taaaaagtga tgttgaaata
    47221 ggagatacac ttactatagg gaatagagat tattgtatag tttcagttgg tgatattgct
    47281 cagaagactc ttagagaaat tgggcattgt acaatcaaat ttgatggaaa atgtgaagta
    47341 aatttaccag gagaaataca tgtggaaata ggaaatccag atataactat tggagattta
    47401 ataactattt cttaatttga aaataaaatt attataacag gaggaattgg tatgttagat
    47461 ttaaacagaa aacttgcaaa attggaagag gaaggtaaaa aaataaaagt agctcttata
    47521 ggagctgggc atatgggaaa tggaatggta agccaaatgg caaatatgaa aggtattgaa
    47581 gcttctattg ttgtagatat aaatttggag ttggctcata aagcttttac tgatgcagga
    47641 atagatgaag aaattataga taatgtaaca aatgaaaagg atgcagaaat aaaattacaa
    47701 gaaggaaaag tattgacatg taatgatttt cttgtggctt gtaaaactaa gtcaatagat
    47761 gtagttatag atgcaactgg aggtattgca attggtgcag agatagcatt aaactctatt
    47821 ttaaataaaa aacatatagt tatgttaaat gctgaaactg attgtgttgt tggaccaata
    47881 cttaaaaaat tagcagatga tgctggagta atatttacag gttctgcagg agatgagcca
    47941 ggtgcagtaa tggaattatt tgattttgca gatgcaatgg ggtttgaagt tagagtgata
    48001 ggtaaaggta aaaataataa attagattta gattgtaatc ctgatacagt tagagaagaa
    48061 gcagaaagaa aaggagcttc acctcatatg atagcttctt ttaaagaagg aactaaaaca
    48121 atggtagaaa tggctttaat gtgtaatgca actggatttg tgcctgatgt aagaggtggt
    48181 catggtattg aagctacagt taatgaagta cctaagaaat atgcattaaa aagcgaaggt
    48241 ggagtattag ataattatgg tgtagttgat tttgtaaatg gtatagcacc tggagttttc
    48301 gtagtagtag cccataaatt aaaagcagtt aatgatgaac taaaatattt aagtatgggt
    48361 gatggaccta attacatatt atatagacca tatcatttat gtagtttaga gactccttta
    48421 tcagcagcta tggcagtctt agaccataag gctacaatag tacctaaagc tggactagta
    48481 gcagaagtta tgactatagc taagaaagat ttgaaaaaag gtgagtttat ggatggctat
    48541 ggagcatata catgctatgg tactatagaa aaatacgatg tagcaaaggc tatgaatgct
    48601 gtacctattg gtttgataag taaaaagact aaagttgtaa aagatattaa aaaaggtgaa
    48661 gttataactt acgatatggt agagatagaa aaagatacta ctttatatca tttaagacaa
    48721 ttgcaagaaa aaatatttgg gtaagatttg atggtaatat aaatagatat tagtaaaaag
    48781 ggttgatggt tatttttaaa tcaacccttt taaattttta tatattcttg ttaggatact
    48841 ttctttaaaa gtatattata ggttatttgt agtcgtaaaa attattttga attagttctg
    48901 caattttata ttcacattga aataatatta ttaaaaattt taatataaaa tgaattaaaa
    48961 tgaccaatta tgtattataa tattaactgt aatactaata tagttctatt aattaaagaa
    49021 atatccccca ttaatatata ataaatatct ttaagcgaga gaacttagta aaaaatatat
    49081 cgtattcaat aaaaaatctg tttacagaat ggtatatata gttatataat atactatata
    49141 agatgcgaaa agataaaaag tatagcacaa tgaaagaagg tgattattat tcagcttaat
    49201 gaaagacaac taaaaattat agatatagtt aaggaaaatg aaccaataac gagtgaaagt
    49261 attgcctcca gcttgaatgt gactcgtgct acacttagat ctgatttagc tatattaaca
    49321 atgacaggaa tattagacgc aagaccaaaa gttggatatt tttattcagg agttagtgaa
    49381 ataaatttaa ttggtaaaag tgtaaaagaa aaaacagtag aggatataat gagcatgcct
    49441 gtactagcta agaaggatga aagtatatat gatgtaatag tcactatgtt tttatctgat
    49501 gtagggagta tagttattat agatgagaat gaagagttat gtggagttgt ttccagaaaa
    49561 gacttgctaa aagcaactat aggaggttct gatataaata aaatgccaat aggtatgata
    49621 atgacaagaa ctccaaatgt agtcacttta actaaaggag caagtgtttt attagcttca
    49681 agaaaaataa tagaacatga agtagattcg attccgattg ttgaatataa gggagaagac
    49741 aaaaatcata tgagagttgt tgggagaata tcaaaaacca acataactaa gctattctta
    49801 gaaatagttg acaattaagg aggtatgctg gtgaaaaacc taattatata tgcagtatca
    49861 gattcagtag gggagactgc acaacaagtt gcaaaggcat gtatgtctca gttttatgtg
    49921 aatgaaactt atgaaataaa aagatttcca tacatgatta ataaaggagt actacttgaa
    49981 actttggaga atgcaaaagc tgaaaacgct ttaattgtat atacattagt tgatgaagaa
    50041 ttatgcagta ttgttgaaag atattgcgaa agagaaggtc ttagttgtat agatttaatg
    50101 acagatatac taagagaaat aagcaagaga acaggaagaa aacctaaaag agaagctggt
    50161 ataattagaa aattagatga atcttacttt aagagagttg aagctataga atttgcagtg
    50221 aaatatgatg atggaaaaga ccctagaggt gttcttcaag cagacataat tttagtggga
    50281 atatcaagaa catctaaaac tcctttgagt atgtatttag ctaataaaaa tataaaagta
    50341 gctaatgttc cattagttcc agaaatacca attcctaaag aagtgtttga gatagatact
    50401 aaaaaaataa taggtcttac taattcacct gaaaagctga atgagataag aacccaaaga
    50461 ttaaaagctt taggattgtc cagtaaagca aactacgcta atttagaaag aatacttcag
    50521 gagttagatt attcagaaga gataatgaaa agaattggat gcccagtaat aaacgtttca
    50581 aataaggcaa tagaagaaac agcaggtata attttagaca taatgaaaga aaatggtcta
    50641 aagatataca aagaaataga aatttaataa tgtctattaa taaaaatgat ataaattaaa
    50701 ataaaaagat taatggggga tttaacaatg gaaactaagt atgtttatag ttttggtgaa
    50761 ggaagcaaag acatgaaatc tttactagga ggtaaaggtg ccaatttagc agaaatgact
    50821 aagataggct tacctgtccc tcctggattc actataacaa cagaagcatg taatgattat
    50881 tatgttaaca atgaaagtat aagagcagag ataataaaag aaatagaaga gcatttagct
    50941 actttagaaa aagacttaaa taaaacatta ggatgcaata agaatccatt attagtatca
    51001 gttcgttctg gtgcagtatt ctctatgcca ggaatgatgg atacaattct taacctaggt
    51061 cttaatgaca atagtgttgt tggcttagca gaggctacac aaaatgaaag atttgcatat
    51121 gatagttaca gaagattcat acaaatgttt tctgatgttg ctatggaagt tccaaaatat
    51181 aaatttgaaa atgtattaga cagagtaaaa gaagcaaagg gatacacagt tgatacagaa
    51241 cttacaactg atgatttaaa agaaatagta aaagaattta aagcaattta taaaaaagaa
    51301 ataaaaaatg atttcccaca agacccaaaa gagcaattaa tgcttgcaat agaggctgta
    51361 tttagatcat ggaataatcc tcgtgcaatc gtataccgta aattgaatga tatagctcat
    51421 aacttaggaa cagcagtaaa tattcaatca atggtatttg gtaatatggg agaaactagt
    51481 ggtacaggtg ttgcatttac tagaaatcca gctacaggag aaaataaatt atttggtgaa
    51541 tttttaatga atgcacaagg tgaagacgtt gttgcaggta taagaactcc tcaaaatata
    51601 tcaactcttg cagatgtaat gccagctgta ttcgatgaat ttgtaaaaat tactcatata
    51661 cttgaaggtc attataaaga tatgcaagat atagagttta ctatagaaaa tgaaagatta
    51721 tatatactac aaactagaaa tggtaagaga acagcagcag cagcaataaa tgtagcggtt
    51781 gatttagttg aagctggaat aattgatgaa aaagaggcta tcatgagaat tgagcctaat
    51841 caattagacc aattattaca tccaaaattt gaagataagg cattaaaaga agccaaggtt
    51901 atagcaaaag gtttaccagc atcaccaggt gcagcaagtg gtaaagttta tttcaatgct
    51961 gatgatgtag taaaagcaaa tgaaaaagga gaaaaagtag ttttagttag actagagaca
    52021 tctccagaag atatagaagg aatggttaaa gcagaaggaa tccttacagc tagaggtgga
    52081 atgacgtcac atgcagcagt tgttgctaga ggtatgggta aatgttgtgt tgctggatgt
    52141 ggagaaataa aagttgatga gtttaataaa gaaataagag cacttgatga tgtagttata
    52201 aaagaaggag aatacatttc tatagatggt tctacaggaa atgtgtacct aggagatgtt
    52261 aagaaaacag aagtatcttt aactggaaat tttgaaaaat taatgaactg ggtagacaaa
    52321 cacaaatgta tgatggttag aactaatgca gataatccta gagatgctcg tgcagctata
    52381 gagtttggtg cagaaggtat aggtctatgt agaactgagc atatgttctt tgatgaagct
    52441 agattgccag cagttagaga aatgatatta tctaatacag tagaacaaag agaaaaagct
    52501 cttgaaaaaa tattaccaat gcaaagagag gattttgtag agttatttaa agttatggat
    52561 ggaaaaccag ttaatataag attactagac ccaccactac atgaattttt gcctcatgat
    52621 gatgagacta tagaagaact ttcaaaatct atgggaataa aagtaagtga tattaagaag
    52681 agaatagttg atttagatga gtttaaccca atgcttggac acagaggttg tagacttgca
    52741 ataacttatc ctgaaatatg tgtaatgcaa tctaaagcta taatacaagg tgctatagaa
    52801 gcaatcaaag caggtgttaa agtttctcca gagataatgg ttccattagt tggagaagta
    52861 aatgagttaa aaataataag aaaaatgata gtagaaactg ttgacgctat aataaaagaa
    52921 gaaggtatag aagttcctta tactgtaggt actatgatag aaatacctag agcttgttta
    52981 actgctgatg aaatagcaca agaagcagac ttcttcagtt ttggtactaa tgacttaact
    53041 caaatggcat ttggatattc aagagatgat gctggtaaat tcttaggtca atatgtagat
    53101 gaagaaatat tagaaaaaga cccattccaa gtactagacc aaaatggtgt tggtaagtta
    53161 gttaagatgg gagctaaatt aggtagagaa gttaaaccag aattaaaact aggaatatgt
    53221 ggagagcatg gaggagaacc atcttcagta gagttctgtt atagtgtagg acttaactat
    53281 gtatcttgtt caccatttag agtgccaatt gctagacttg cagcagctca agcatctata
    53341 aaaaatccta gataataata gactgcaaaa tatgtaacta aataaaaata aaatccccta
    53401 ttgttcaatg tatcaaatta agtatttgta aattgaatat aggggatttt tattataaaa
    53461 atttaactta tatagttgta agtgaaacgt atttaattat attgcatata ttattgtaga
    53521 gagaaatttc aactatcaat taaattgagt ttactggagg ggataaagtt gacaaagtgt
    53581 cctaaatgtg gaaaggaagt atcatcacgt ccaggtagta tatgcccacg atgtggattt
    53641 aaagtagcag atgagagtct aaaaataaga tgtccagaac ctagttgtag ggctttggta
    53701 tctaggaaac tggattattg tcctaaatgt ggatgcaaac ttagaggata taatatttct
    53761 gaatttgtta acatggtgag atgtaaaata gatgaaacat ttgataaaaa ataattaaat
    53821 ttagagttaa gcttttgagg gtgtctcaaa atgaactttt tagttcagaa ggcactcttt
    53881 ttgtatggaa tcaaatatat ttttataatt taaacaataa aaaagaggct tatctctatt
    53941 ttgagatagc ctctataaaa gtatacagtt ttataagtaa atatattttt actaatctat
    54001 ttctattatt tgccttctga atcttccatg actaaagcca cgagtatgca taactatttt
    54061 ttaatcaaac gtatgttaaa gtaatctttc tatttaccag aattattttc cagtattaag
    54121 tatccaaggt ttttctatac cattttttag tgagttataa ttcacaataa gagtgtcata
    54181 gctattttta gcatcttcat tacttaccac ttgtttttta taatcagtta gagtaattaa
    54241 tcctaaatca tatcttaatt tagtattttt taattgtttt tctgataaat caagttgttt
    54301 tttagtaatc tgaattgtat cttctaaagc aagtaattga gcatagctat caattaaccc
    54361 ttttttaaga gcttttttac tgtcttctaa tgttactaga ccattttcag tattaaattt
    54421 ttgttgaaga taatttgcat aagtagtaag gccagcagca taagtctgtc tttcaagatt
    54481 gtatgcttca acttcttttt tatatctatc ataatctttt tcatacttat ccataagaac
    54541 cttgtattca tcatcagtta ggttaccttg ctctggtttt gtaggagcaa ctggtcttgt
    54601 aaggttaggt ctatctggta aatctgcttt gttctcatct ttatcattaa agctttcttc
    54661 tgtacgttct aaaatcaatt ggtcgtattt taaatattca ttaaccttac cttccatata
    54721 tgaatcaact gagccaccta ctctaaaaga ttcaaatcta tattctttat ccaattgata
    54781 attatttaaa tctatattag ttaataactt aaaatagtct tgattgtttt ttagttcttg
    54841 aagtttagct ttttgagtat tttgtagtgt ttgaagttca agttcagctg attcagtttc
    54901 taaagatgtt actaacccta aatctttttt taatttcata tctttaattt ctttagtttt
    54961 tatttcaaga ttgtttttta ttttatctag ctcattttct cttgaaacta aatcgttaaa
    55021 tttattagta atatcatttg ctatttggtc ctccataaag cctttagcct gttcactttg
    55081 atttttaaga agtccaattt ggtcgtatgg aaacgagttg tcacttccag taaaactatc
    55141 aaactcatct tgtacttcta acttttcttt taacattttt atttcttctg atttcatttt
    55201 taatttttca ctatttttaa gtgcagcact aacagcttct ctagcagtca aaatttcctt
    55261 ttcagatgtt cttttatttg agtcacttaa aatagaatta tcaactttgt ttatagaaga
    55321 agtattatta tctgcgtaaa ctgggattaa attggcagtt attataccaa caccacagct
    55381 aattgcaact attttgctca tttttttatt caatacaatc accctccatt ctatttaaac
    55441 tttagctttc aattttggtt tctttattat tttacgtttt atacttccta ttttatctgt
    55501 tatcttacca aatattttag gcattttgtc tgaaaactta ttttcccatt tatcaaatat
    55561 taagtatata gtaggtataa gcaccaaagt tataagtgta gatacagtta gaccacccat
    55621 tataactatg gctagtggtt gcatagtttc tccaccttca ccaagagata gtgctgttgg
    55681 caacatacct aaaattgttg ttgctgtagt cattaagact ggtcttaatc ttgctgcaca
    55741 accaattgaa accaaaccaa ttaaatcatt gttacgattt gtttttctaa gttgctctat
    55801 gtagtcaata agaactatac cattattaac aacaataccg ataagtaaga ttatacctag
    55861 cataccgaca gtacttaaac tttgaccact aactagaagc gaaagcacaa ctccaacgaa
    55921 tgcaaatgga acacagaaca tgattataaa tggtttacta aatgattcaa actgagctac
    55981 cattaccata taaactaatg ctatagctat gaccattgaa aatccaagac ttgccataga
    56041 ttcattcatc atttctgttt caccaccaac acctatagag taatctttag gaattaccat
    56101 tttattcgtt ttttctagtg ctagttttgt agcagtacca gcatctaaat tgtcaagtgt
    56161 agcagttata tttacagaaa aatctccatc agtcctagat atagattgta gaccatctgc
    56221 catttttata tctgaaaagg caccaagagg aacttctttt ccagttgcag agtttacttt
    56281 tagttgcttt atatcttcta agctatcaat agtatctgct ttaaatttta ggtttacatc
    56341 cactttgtaa tcatcaatag tagcagtggt tacggagtca ccatttattg ctgtacgtaa
    56401 catgcctgca atttcagcgg tatttacacc atattcttga gccttatgtt tatctatagt
    56461 aaattgagct tcttgtgttg tatctgtaag agaagtttca acatttctaa atccttttat
    56521 agagctaaat tctttttcag cttcttttcc cagtattttt aatacatcta aatcaggacc
    56581 tttcatttct aatgtgaagt ctgctcctcc agctccaccc ataacactac tgttttgact
    56641 tacatttatt ttacaatctg gtacagtagc cactctatca attatctctt tttctatttc
    56701 atcagtagtt ctttttctat ttttttgaga ttctaattca attgctatac ttgctttact
    56761 attatttgaa tctgatgtca aagttgttat taaagtttta atttctggaa tatctgagac
    56821 ttttttctca gtcatagata catagtaatc attggattct aggctaagac cttttggaag
    56881 ttctatagaa atagataatt cacccttgtc tgctgcaggc ataaagtcca tacctataaa
    56941 tcccattcca aataaggaag ctacaaaaat aagtaaactt gatattacaa ctaagcgttg
    57001 atgtctcaaa catacactta ataatttttt atatgcttca cttaccttat caaatatagg
    57061 actatctttt tcttgcatct tttgactatt tttttctctg cttaattttg caaatacact
    57121 aggaacgagt gtaattgcac ctataaagga gaatgctagt gaagctatta gtgttttacc
    57181 taatgcccca aaagttattt tagctatacc ttctgtaaac atgataggta agaatattgc
    57241 tattgtggtc aatgttgatg caagtattgc atttgttaca gttcttgttc cctcaatagc
    57301 acaatcatca agactcaatc taccactttt cctatattta aatatatttt caattacaac
    57361 tgtagcatta tcaacaacca taccaactga aattaccaat gaacttgcag taataagatt
    57421 taaactttca ccagtaaagt agagaattgc tatagcacct attatagaaa ttggtattgc
    57481 aactgcaaca actagtgaag ctcttacact ttttaggaaa gcaaatatta caataaatgc
    57541 gataacagca cttataagca agttgctcat tacactgttt attgattcac ttatgtatga
    57601 acttgtatca ttagcaattt taattttaaa ttgaggattt tccttattca attcatctac
    57661 gtatttgtat gcactctgca taacttttac agtgttggca tcttgttgtt tagaaatatc
    57721 tattaccaaa ctgtcttttc cattgtatct ataaatacta tctttgtctg catatccata
    57781 atctacacta caaagattat ctaatctaac tgtatttcct ccttttacag gaatttgaat
    57841 ctgttttata tcatctaggg attctaattt atcaatcgct cttaaaacta ttttatcctc
    57901 accttgagta attgaaccat aagggaatgt tttattacta gaggataaga caccttttat
    57961 agtggataaa ctaactccat aatttgaaag aacagctggg tcagctatta cattaacctg
    58021 tgctttttct ccacctttaa ggtctgctga agtaacacca tctatggatt ctattttagg
    58081 ctgaattaca tcttcagcgt attttcttaa ggcttgtgaa ctattttcat ttcctccaga
    58141 tatgacaagt tgacctatag cctgtgcatt catatccagc tttaaaactg ttggtttagc
    58201 tgcatcatct gggaggctgg tctgtaatgc atcaacttta gacctaacat cattcattat
    58261 ttcatccaaa ttagttccaa attcaaactg agcaactacc atagaaacac tttcttgtga
    58321 acttgatata gtagatttta catttgaaac ggcagataaa ctttcatcaa ctttttgact
    58381 aacttgttca tcaacgtctt gaggaccagc acctgtccaa gtagtcatga ccattacaac
    58441 tggtatttct atatcaggca ttagatttat tgacattttt ttatatccaa taccaccaaa
    58501 aactattaat actaaaaaga ccattatgat tgtgagagga cgtttaacgg aagtctgtgt
    58561 taaattcata gtctacttct ccttttctac aggaaatatc ttagttccgt cagaaataag
    58621 acttatccct ccgataatca ctgtatcttt atcacttaca ccacttttaa tttctatttt
    58681 atcctctgtt acaattccag tttgaaccaa gtgtttcttt gctatattgt cttttgttgc
    58741 tatgtataca tattttttac cattttcttc aaatattgct tttttaggta ctgtaacagc
    58801 accattttct ttttcaacac ttaagtttac ttgagcagac atacctgcct taaatttgtt
    58861 gtttgtatta tttacaagta ctttaacagg atataaagat gtctgagggt caactactga
    58921 aggtacataa tctatttttc ctaaaatcct ttggtcttca attataacat ctacatttcc
    58981 atctttctta aatttaccta tatctgattc tgctacattt aaatcaactt ctagtatatt
    59041 aggattagaa ataataaaag ctggttgtga ttgtgttatc atttcctttg aatcaaagtt
    59101 tttagctgtt attgtaccat ctactggaga tgttaatgtt aatttgtcta aattactcat
    59161 tgctaaatta tatgatactt gagcttggtc aagctgtttt ttagcaagtg ctttagcttg
    59221 aggtatactt tttgaagttg ctgtatcgta tgctctttga gcagaatcta actgttgttt
    59281 tgtttgctct aaagaaaatt ctattttttt aaattggtct aaactaatga cttcctgttc
    59341 ataaagaatc ttattattgt cataatttct ttgagactcc tcatattgaa tctttgcatt
    59401 gtcaagagct gtttttgcct gatttacttg tgcctctaat gaaccaccag tggcacttga
    59461 ataacttgct tttgctaaat ctagtgaggc tttactttgc tttacactat tttctagctc
    59521 tggactgctt atactcaata attcatctcc ttttcttaca ttttgcccaa gttttacata
    59581 cacttcttga actgtgccaa cagtctgtgc agtaacagct gtttcatctt taacctttgt
    59641 agttcctgag aaagaatttg tgttttcaat ttctccacct attgcttttt gaacagaaac
    59701 tgctattggt ttctcaggtt cagattcttc tgtttttcca catccaacta aaaacagtga
    59761 actacacatt aagaaaacta ttaaatatct tttcatatct cctcctatta tatattgcac
    59821 actagcttgt tgtagaattt aaagtgtctt ataatttaag tttagtttta gactaattaa
    59881 aaaacttccc tttattggca atttataatt ctctaatttg attataaatt tataattatt
    59941 atgagtcaat aactttttct tgttttaaat ttattaatag tttataattt atactatatt
    60001 aaacaacaat gttttattat attatctatt gaagggatgt tacaaatatg gataacaaca
    60061 atatacttaa aatagcggac tcttttttag attttttatt tgtgcttgaa aataatatat
    60121 ttaaagaaag cgatttgtta aaaaagtttc aaaataactc tgatataatg aaagcttatt
    60181 ttgaagaatg tccaatggct ccatcacatg ctaaggtaat tctttatctt atgacatcaa
    60241 attcatcatc aatttctcaa atagcttcta atttaggaat tttaaaatca aatatgactc
    60301 cgattattga cagattagtg gaacatggtt tagtcaataa atttcctgac ccaaaagata
    60361 gaaggattct tagggttgaa ttaacagata aagcatttaa attatttgat gcagtaaaag
    60421 ctattctcaa agaatctcta gtaaaaaaat tatctaatct ttctgaagag gaactaactt
    60481 tattagatga acatacatta aagttatcag aaatagtaaa gaaattaggt tagagtgtga
    60541 ttgtaaaagt cactattaaa aaagttgaaa cttc
//
LOCUS       DAEPIN010000016        58773 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.016, whole genome shotgun sequence.
ACCESSION   DAEPIN010000016 DAEPIN010000000
VERSION     DAEPIN010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 58773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58773
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.016"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            complement(121..894)
                     /locus_tag="KR250_001178"
     CDS             complement(121..894)
                     /locus_tag="KR250_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SigB/SigF/SigG family RNA polymerase sigma
                     factor"
                     /protein_id="HBH0067142.1"
                     /translation="MKNVANATHYLNMDTKELFNLYNKNKNVDIRNILIERHLYLARL
                     LAKKYINKGVDFEDIYQVASLALIYAIDRYDVEKGFEFSSFATPTIVGEIKKYFRDKV
                     WTLRVPRRIQELSKKISDAKIKLEQENKKHPKVKDIADYIGVSEEDVLEAMEASYGYQ
                     PMSLDSSSNDDSEDKDITLIDKIGKEEGNFGSIEYEDFINKFIETLNELEVKIFKDRF
                     FFDKTQSSIAKELGISQMTVSRLEKKVVEKLKKEYEKNL"
     gene            complement(894..1280)
                     /locus_tag="KR250_001179"
     CDS             complement(894..1280)
                     /locus_tag="KR250_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBH0067143.1"
                     /translation="MEITTNPDYVSIIRLTASGIANKMGFPIDDIEDIKVAVSEACTN
                     AIKHSKDDVFNIVFNILDNAINIEIQDNGKGYDVSSISTPDLENPKESGLGLFIIKTL
                     MDDVDIESEHNQGTKIKMTKYLGVDI"
     gene            complement(1305..1622)
                     /locus_tag="KR250_001180"
     CDS             complement(1305..1622)
                     /locus_tag="KR250_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HBH0067144.1"
                     /translation="MSMNIDSNLDSQNKFWNVCLDGELDVSTADKLKEHLHALIEKNM
                     LDVKINLKDLDYIDSTGLGAMIGVLKKLKINEKEIYIVNPKSNVRKIFTITGLDKIFK
                     VEG"
     gene            complement(1634..1894)
                     /locus_tag="KR250_001181"
     CDS             complement(1634..1894)
                     /locus_tag="KR250_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HBH0067145.1"
                     /translation="MNAWGWQIGAVLVGSSALIVAIYLAKTLNSINKVVEKAYKIVDY
                     NERHIQDIVENASSISKGIDDIVYVINKILSIGNIFKIIKRK"
     gene            complement(1894..2190)
                     /locus_tag="KR250_001182"
     CDS             complement(1894..2190)
                     /locus_tag="KR250_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBH0067146.1"
                     /translation="MCNKRSCKKGGFLLLGAILGFIFGMFFAPKKGSELRKETKEKFN
                     DVKENPKEVLHETFNDVKERIINLVDDDNNEEDIKISEEDIVISKSFDDEGDVN"
     gene            complement(2259..4685)
                     /gene="gyrA"
                     /locus_tag="KR250_001183"
     CDS             complement(2259..4685)
                     /gene="gyrA"
                     /locus_tag="KR250_001183"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HBH0067147.1"
                     /translation="MEENNKILPIEIAEEMKKSYIDYSMSVIAGRALPDVRDGLKPVH
                     RRILYSMSELNLTPDKPYRKSARIVGDVLGKYHPHGDTAVYYAMVRMAQDFSTRALLV
                     DGHGNFGSVDGDSPAAMRYTEAKMSKLSLELLRDIEKETVDFKPNFDESLKEPSVLPA
                     RYPNLLVNGSNGIAVGMATSIPPHNLAEVIDATVYLIDNPECSVDDLIKFVQGPDFPT
                     AAIIMGKESIAEAYRTGRGKVKVRSRAFIEELPKGKQQIIVTEIPYQVNKAKLVERIA
                     ELVKEKRIEGILDLRDESNRNGMRIVIELKRDANANIVLNNLYKHSQMEDTFSIIMLA
                     LVDGQPRVLNLKQILYHYIKHQEDVVTRRTKFELNKAEARAHILEGLKIALDNIDAVI
                     SLIRASKTGQEAKLGLIEKFKLTEIQAQAILDMRLQRLTGLERDKIEAEYEDLIKKIN
                     RLKEILADERLLLNVIKDEITIIKENYSDERRTEIRHAEGEIDMRDLISDEEIAITLT
                     HFGYIKRLPSDTYKSQKRGGRGISALTTREEDFVRHLVTTTTHSRLLFFTNKGRVFKL
                     NAYEIPEGKRQAKGTAIVNLLQLSADEKIATLIPIDGNDENEYLLLATKKGIVKKTKR
                     EEFKNINKSGLIAIGLRDDDELIGVELTDGKQEVLLVTKEGMSIRFDENDIRYMGRTA
                     MGVKGITLSKEDFVVSMNLCSKGTDVLVVSKNGFGKRTNIEEYRSQIRAGKGIKTYNI
                     SEKTGTIVGADMVNEDDEIMIINSDGVLIRIRVNEISLFGRVTSGVKLMKTNDEVNVV
                     SIAKINIEEE"
     gene            complement(4705..6606)
                     /gene="gyrB"
                     /locus_tag="KR250_001184"
     CDS             complement(4705..6606)
                     /gene="gyrB"
                     /locus_tag="KR250_001184"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003226808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HBH0067148.1"
                     /translation="MKQEYGASQIQVLEGLEAVRKRPGMYIGSTSPRGLHHLVYEVVD
                     NSIDEALQGYCSDIYVSINEDGSVLVKDNGRGIPVEIHPKTGKSTLETVLTNLHAGGK
                     FGGGGYKVSGGLHGVGVSVVNALSKWMVAEVYLNGKRYKQTYEKGLPTSKLEVVGESQ
                     DKGTMIQFMPDETIFDEIEFKYETLEYRLRELSFLNKGIKIVFEDKREGQEKRKEFHY
                     TGGLVEYIKYLNKSRTGIHDDIVYIDKKVDDCFVELAMQYTDGYTENIYSFANNINTH
                     EGGSHLSGFKAALTKTVNDYAKRNKFLKENDVNLLGEDIREGLTAVVSVKLPEPQFEG
                     QTKTKLGNSFMRGIVDSVTVDELGSFLEENPSTARIIVDKALRAQRAREAAKKARELT
                     RRKSVLESTSLPGKLADCAEKDPSKSEIFLVEGDSAGGSAKQGRDRNSQAILPLRGKI
                     LNVEKSRLDRILSSDEIKNMITAYGCGIGEDFDIDKARYHKIIIMTDADVDGAHIRTL
                     LLTFFFRYMRPLIDEGYVYAAQPPLYKVTKQKKEHYVYSDKELNILLDEIGRNGVELQ
                     RYKGLGEMNAEQLWETTMNPETRTLLQVTVEDAAIADEVFSMLMGDKVAPRKEFIEEN
                     ARFVRNLDI"
     gene            complement(6607..7722)
                     /gene="recF"
                     /locus_tag="KR250_001185"
     CDS             complement(6607..7722)
                     /gene="recF"
                     /locus_tag="KR250_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HBH0067149.1"
                     /translation="MKLKSLQLVNFRNYKKLHLEFNGKVNLLVGKNGQGKTNIVESIY
                     MLSFGKSFRTNKDKEMVRFNSENLYIGGSFSKYNKYSLIELIIGKDKKGIRINKVPLQ
                     KIQELLGNLNVVIFSPEDLRLVKEGPKERRAFIDKEISQIIPKYYKYLTNYNKTLSQR
                     SRVLKNIHVDEALLDVYDDTLAKYGSYIYILRRDFIKKIANISENMHMNLTNGVERLS
                     IRYKNQINITDEDTIDTVYNKFLAKLSSNRPNDIESKTTRYGIHKDDLNIFINDLDAR
                     LFGSQGQQRTASISLKLSEIELIKNEVEEYPVLILDDVFSELDEARQKLLVNNLSNVQ
                     MFITSAEVSHKKIFDEKNVTIFNIENGDVISIENGGN"
     gene            complement(7739..7945)
                     /gene="yaaA"
                     /locus_tag="KR250_001186"
     CDS             complement(7739..7945)
                     /gene="yaaA"
                     /locus_tag="KR250_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HBH0067150.1"
                     /translation="MTEITIESEYIKLDQFLKLAEIASTGGHAKFLIQEGLVTVNDEI
                     ELRRGKKIKSGDIVEIEGTKIKVL"
     gene            complement(8082..9188)
                     /locus_tag="KR250_001187"
     CDS             complement(8082..9188)
                     /locus_tag="KR250_001187"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HBH0067151.1"
                     /translation="MKIICNQKILANRIGIAQKAINGKTTIELLKGILISTEEGQLKL
                     TGYDAEIGIETYVQAEIIEKGDVVVDARLFGDIIRKLPDSFVEIETDSENNIYINCVN
                     SRFKIKGYAAKEFPKLPELNEEDLYSIPQEILKNMIKQTVFAISQDQTKPVLMGELLE
                     IVDRNLNLVAIDGYRLAVKSCSVDSLTENIKVIIPGKTLIDVNSLLSGEDNVKVGFNE
                     KNAIFIINDTKIITRLLEGDFIDYKKLLPREHNSRVKLNTKELLNSIERASLLSQSEK
                     NNLIKLSIRDKVMAITSNTEKGNVYEEVEIDLDGDYLDIAFNSRYFIEGLKNIDNEEI
                     FIEFTTNVNPCIIKPTDDVNYIYLLLPVRISSNI"
     gene            complement(9430..10752)
                     /gene="dnaA"
                     /locus_tag="KR250_001188"
     CDS             complement(9430..10752)
                     /gene="dnaA"
                     /locus_tag="KR250_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HBH0067152.1"
                     /translation="MMDIVSLWDKTLQLIKGDLTSVSFNTFFKNIVPLKIHLNDLILL
                     APSDFNKDILENRYLHLIEDAISQLSLKKYNIKFVLSEKEVADLNSDSTDLNYRVLYP
                     NLNPKYTFDTFVIGNSNRFAHAACVAVAESPAKAYNPLFLYGGVGLGKTHLMHAIGHH
                     IVSQKKDSKVVYVSSEKFTNELINSIKDDKNEEFRNKYRNVDVLLIDDIQFIAGKERT
                     QEEFFHTFNTLHEANKQIIISSDRPPKDIPTLEDRLRSRFEMGLITDIQAPDFETRIA
                     ILRKKAQLERIDVPNEVMSYIAKNIKSNIRELEGALTRVVAYSSLSNRVISFDLATEA
                     LKDIITTSKNEEINVLRIKEKVSSVFNLKMEDFNSKKRTRSIAYPRQIAMYLTRELTD
                     LSLPKIGEEFGGRDHTTVIHAHDKVSKDIEESEEIKTKIDKIISDLKG"
     gene            11408..11545
                     /gene="rpmH"
                     /locus_tag="KR250_001189"
     CDS             11408..11545
                     /gene="rpmH"
                     /locus_tag="KR250_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HBH0067153.1"
                     /translation="MSKRTYQPKKRQRSKEHGFRKRMKTSNGRNVLKRRRAKGRNRLT
                     H"
     gene            11623..11967
                     /gene="rnpA"
                     /locus_tag="KR250_001190"
     CDS             11623..11967
                     /gene="rnpA"
                     /locus_tag="KR250_001190"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HBH0067154.1"
                     /translation="MDFNRTKGLKKDSDFRKVYKHGKSFANKYLVIYILKNKSDYSRV
                     GISVSKKVGKAITRNRVRRLIKEAYRLNIDEKIKPGYDIVFIARVSSKDATFKDIDKS
                     IKNLVKRTDISI"
     gene            11999..12250
                     /gene="yidD"
                     /locus_tag="KR250_001191"
     CDS             11999..12250
                     /gene="yidD"
                     /locus_tag="KR250_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HBH0067155.1"
                     /translation="MNKIIYILKEISKHLSNLCIYLVRFYQKYISPLKGPTCRFYPTC
                     SQYSIEAFKKYGFVKGMYLTIRRLLKCHPFHPGGYDPLK"
     gene            12282..12989
                     /locus_tag="KR250_001192"
     CDS             12282..12989
                     /locus_tag="KR250_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HBH0067156.1"
                     /translation="MNIIGNFLGLILKVIFEFVNHYGISIILFTILVKIILLPLTIKQ
                     TKSTKAMQDIQPRIKEIQEKYKNKPEKQNEEIVKLYGEAKINPLSGCLPLLIQFPILI
                     GLFSVLREPVAHGVFANKAAFLAADNGFLWIKSLTSPDYVLAVFSGASAYVMQKVMTP
                     KDQLQGSMKVMTYVMAGMSFYWGFIFPAGLTLYWTVSNLFSIAQYYLIMNPLKAKLAA
                     NSKEEKVNEKKPKINKK"
     gene            12958..13587
                     /locus_tag="KR250_001193"
     CDS             12958..13587
                     /locus_tag="KR250_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein jag"
                     /protein_id="HBH0067157.1"
                     /translation="MRKSLKLIKSKSKEEAISKAIAELNLKAEDIEVEILENPSKGFL
                     GFIGAKDGTYEIFVIEREVDVAKNFIEVMLKNANVDAKVNVSQKDNLIMVDIEGKEAA
                     SLIGRRGETLDSIQFLTGLALNKINKDSHTRVLVDIENYRSKREESLIRYANKVAREV
                     AKTRKTKKLDYMNPYERRIIHSALQNDKYVITYSEGTDPYRRLVIECKR"
     gene            13699..15078
                     /gene="mnmE"
                     /locus_tag="KR250_001194"
     CDS             13699..15078
                     /gene="mnmE"
                     /locus_tag="KR250_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HBH0067158.1"
                     /translation="MFIDDTIAAIATAPGEGGIGILRISGEKALKVAEEIFKSMSGKS
                     IEEYNKRTLIYGNIVDNENVIDEVLLAYMKGPNSYTGEDVIEINCHGGFISVKKILEL
                     ILSKDVRLAEAGEFTKRAFLNGRIDLSQAEAVIDVIKAKTDIAHEVAQNQLEGSLSKK
                     IRELRDKVTEILAHVEVAIDYPEEDIEHITYQTLKEKTDELKKDIKKLYDTAESGKIL
                     REGLKTVIVGKPNVGKSSLLNSILGENRAIVTDIPGTTRDVIEEFVNIKGIPLKIVDT
                     AGIRDTDDIVEKIGVEKSKESFTSADLIVMVLDASRKLSEEDIEILEKLKDKQTIVLL
                     NKNDLKQEIEEEKILKYVENNSIIKISALQQEGIEELQDKIESMVYKGSIKNNSSLVV
                     TNSRHKDALSKAYKSATDALIALEQSMPFDFVEVDLKNIWDYLGYINGDTVTEDLLDN
                     IFHNFCIGK"
     gene            15176..17068
                     /gene="mnmG"
                     /locus_tag="KR250_001195"
     CDS             15176..17068
                     /gene="mnmG"
                     /locus_tag="KR250_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HBH0067159.1"
                     /translation="MKFEAGKYDVIVVGAGHAGCEAALATARMGYKTLIITMSLDSIA
                     LMPCNPSIGGTGKGQLVKEIDALGGQMGLNIDKTYIQSRMLNTAKGPAVHSLRAQADK
                     FKYHEEMKKTLEDEPNLDIAMDEVVEILHEGNVVIGVGTKLGCSFKSKAVILATGVYL
                     NSKIYMGEVAFYEGPNALGYAKYLTDSLVELGLRMRRFKTGTPARVHRDSIDFSVMSL
                     QEGDEKVTPFSFMNENIEKKQEPCYLTRTTEETQKVILDNLKRSAMYSGVIESTGPRY
                     CPSIEDKVVRFSDKTSHQLFIEPEGLNTKEMYIQGISTSLPFEVQLDMYKTIKGLENC
                     KIMRPAYAIEYDCVDPTQLKISLEIKGVENLFSAGQFNGTSGYEEAAAQGLMAGINAV
                     RKIEGKEPFVLDRSEAYIGVLLDDLVTKGTNEPYRMMTSRAEYRLYLRQDNADMRLTQ
                     KGYDIGLVKKDRYERFLNKKAAVEKEFERLKNERVTPKEVNSLLEEKGATPIKVGISL
                     YEFLKRPEVTYELLEELGKGAGEDVSREVKEQCVIITKYEGYIEKQLKQIDQFKKLEN
                     KKLDEKINYSSIEGLRLEARQKLDDIKPISIGQASRISGVSPADISVLLIYLEQIRRT
                     RGGKGE"
     gene            17061..17780
                     /gene="rsmG"
                     /locus_tag="KR250_001196"
     CDS             17061..17780
                     /gene="rsmG"
                     /locus_tag="KR250_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HBH0067160.1"
                     /translation="MNNIELLKNGIEGFNIDTNDSMLEKFKIYREILVEWNKKMNLTG
                     IEDEKEVYIKHFLDSISAVKNGYIKNGMSIIDVGTGAGFPGIPLKICLDSLELTLLDS
                     LNKRINFLEEVSRVLELDNITFIHGRAEDFGKNEDYREKYDVATARAVAGLPILMEFC
                     VPFVKVGGYFICLKGPNANLELEESQKAIDVLGLEYIEKIDVELPEVDLNHNILVFKK
                     VMETPVKYPRKAGKPAKNPIK"
     gene            17943..18728
                     /gene="noc"
                     /locus_tag="KR250_001197"
     CDS             17943..18728
                     /gene="noc"
                     /locus_tag="KR250_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion protein"
                     /protein_id="HBH0067161.1"
                     /translation="MEDKRVMEIPIEDVVPNPYQPRKIFSQVSLEELSNSIKVYGIIQ
                     PITVRAKDGKYELIAGERRLRAAKLAELKTIPAIINNMNDESSAVLALLENLQREDLN
                     FIEEAIGYENLIKEHAFTQQQLAEKLGKNQSTVANKLRILKLPNDIKMKLIENNLTER
                     HARAFLKLPSEDLMQSVLDKVIKNELTVKKTEKLIQDVLEETKVQEEPDKKQNIKGAM
                     SIRIYINTIKQAFDAISNTGIDAKYNEIDKGDYMEVVVKIPKK"
     gene            18863..19636
                     /locus_tag="KR250_001198"
     CDS             18863..19636
                     /locus_tag="KR250_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBH0067162.1"
                     /translation="MGKVIAVFNQKGGVGKTTTNVNLSASLGTLGKKILVLDLDPQGN
                     TTSGYGINKNEVENTIYEIMLDGLHIKEAIISTEFENVDVVPSATELSGAEIELTSKT
                     NREYILKNSIKAVIDEYDYIFLDCPPSLGMLTINCLTAVDSVLIPIQCEYYALEGVSQ
                     LMETIKLVKSRLNADIEIQGVVLSMFDGRANLSIQVVEEVKKYFKGSVYTTLIPRNVR
                     LAEAPSHGKPVIYYDKRCRGSVAYLELAEEFIDLEEEEW"
     gene            19638..20501
                     /locus_tag="KR250_001199"
     CDS             19638..20501
                     /locus_tag="KR250_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HBH0067163.1"
                     /translation="MENKSKRSNRLGRGLSALIPEIKGETSEKEIVNIDIDKIYPNEV
                     QPRKQFDEEKIKVLSDSIKNYGVLQPIVVKMDENNKYMIIAGERRFRASKLANKNQIP
                     AIIKDIDMKDIMEIALIENLQREDLNSIEEALAYKSLIEHYNVTQEEISEAVGKSRPH
                     ITNTLRLLNLGQDVIEMIDSGRITAGHGKALLRIADKDLQLQIAKKIEEEELSVREVE
                     NIAKKISENKQEEPKKSKPKDVFILDVEDKLRNIFGTKVNISKGKKKGKIEIEYYNDD
                     DLNSIVSMLLE"
     gene            20534..21676
                     /locus_tag="KR250_001200"
     CDS             20534..21676
                     /locus_tag="KR250_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HBH0067164.1"
                     /translation="MIYLDNAATTYPKPERVYNAVLDCMKNYCANPGRAGHKLAMRAA
                     REIYDTRENIAKLFNVSNPMNIVFTSNATDSLNLAIKGVLQEGDHVITTSMEHNSVIR
                     PIKALEKRGIENTVVKCDYEGFLDYEDLEKSIKNNTKLIVTTHASNVCGTLIDIKKVG
                     EIAKKHNILFLVDASQTAGVYDIDVNECNIDMLAMPGHKCLFGPQGTGILYVREGLNL
                     NILKEGGTGSKSEEIVQPELFPDKYESGTHNTPGIAGLNQGILFIFERGINNIRQHEE
                     ELCQYMIDKLEEVPDIKIYGPKDSKKRASVIALNIGDMDSGEVTFLLDSDYNIATRSG
                     IHCSPLAHTTLGTLKQGAVRFSIGYFNTKDEIDKAVEALKKISKNK"
     gene            complement(21810..22385)
                     /locus_tag="KR250_001201"
     CDS             complement(21810..22385)
                     /locus_tag="KR250_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HBH0067165.1"
                     /translation="MKNKKIMLVLSILSISIFAVGCTNAQNGSDSSKKETKSNTNVEQ
                     PKEENNTEKEVPNNKAKPTPKEETKAQSATIYSFDVDKTDLIENKVDLNKIDENTLFE
                     ELQKLKVVPESAKLNSFTTKDIDGVKTGILDVSSDFTKSNLGSDAETLMLDSVARTYI
                     KNMNVEQIKITVDGSNYESGHIVLEEGDYLK"
     gene            22645..23937
                     /locus_tag="KR250_001202"
     CDS             22645..23937
                     /locus_tag="KR250_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBH0067166.1"
                     /translation="MEILSLGEKIKKLRKEKNMTLKELAGDRITAAQISHIERDKSHT
                     SYELLEYLGDKLDVSVEYLLETKEMQSKKITDNLILQSEIYIKENELNKAEDQIKEVL
                     DICKRYDLMENYGRCNFLMANINLKKSNYNDAIENFEKALYFFIKNSDSENILKCYLN
                     IGKIYMQEDFYKGAISHFNFAEEILSESKFEDVNIHKELYSKMAYCYIKLDNPNMSLK
                     YIEKINEIDNRNDRKEDVDILVLKANNMLEIGKYEESRDYFKKALKILESEDNKTGLA
                     NVYLTICDVYRKIGETDRVLEYSQKVYDIKKNDEDEYMMSGLFKIIEAYIDKEDYDLA
                     RKYCKIALASSIKNKNKFNEYKALRFYANMYKNQNEVTLAIEYLIKCIKIVSDLGNNR
                     ILANLYIDLGQLYSNISKEKELEYYQKGVFMYKNLEIM"
     gene            24042..24644
                     /gene="yedF"
                     /locus_tag="KR250_001203"
     CDS             24042..24644
                     /gene="yedF"
                     /locus_tag="KR250_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HBH0067167.1"
                     /translation="MSIKIDARGLACPKPVINTKRELDNLEEGVVVTIVDNETAKENI
                     LKLAKSLSCEANIVDEKENFISIEIRKGKNILDKQNVVDNKESELDNTCIFISSDKMG
                     LGNDELGKVLIKGFIYTLTESKPYPKYVLLVNGGVKLSAENEETIENLKILENMGVEV
                     LSCGTCLDYYNLKDKLQVGTVTNMYTIVETLKNASNTISI"
     gene            24717..24980
                     /locus_tag="KR250_001204"
     CDS             24717..24980
                     /locus_tag="KR250_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HBH0067168.1"
                     /translation="MNQMYIVSFNSTHHAIRSEKLFGENSLKVMALPTPREITASCGI
                     SIKFSFEDMEKIKTILVENNVDIKGIYCISKLENGSKEVEKLD"
     gene            24995..25180
                     /locus_tag="KR250_001205"
     CDS             24995..25180
                     /locus_tag="KR250_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HBH0067169.1"
                     /translation="MPMDLKIGDIVELKKQHACGCKEFEIIRTGMDIKIKCTKCSRLI
                     MLDRETLEKRVKKLIKK"
     gene            25283..26479
                     /locus_tag="KR250_001206"
     CDS             25283..26479
                     /locus_tag="KR250_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBH0067170.1"
                     /translation="MAVKYAKRMQGLQGSEIRELLKLTQQPQIISFAGGMPAPELFPV
                     EEMKKVSVAVLEENGRSAMQYTTTEGYEPLREKIAARMNDKNKTNVNKDDILVTSGSQ
                     QGLDFAGKVFIDEGDVILCESPSYIGAINAFKSYQPKFIDVPTDSDGMIMEELEKILE
                     TTDRIKMIYVIPDFQNPTGRTWPLERRKKFMEIVNKFEIPVIEDNPYGDLRFEGETLP
                     SLKSMDTKGLVIFLGTFSKIFCPGYRLGWTCASPEILSKFNFAKQGADLQASTISQME
                     VSKFMDMYDLDAHVDKIKAVYVKRRDVMLKTMEEEFPEGLVFTHPEGGLFTWVELPSN
                     LNAKELMPKCLDKNVAYVAGGGFFPNGGRENTFRLNYSNMPEEKIIEGIKNIAAVLKE
                     AMGVEA"
     gene            26693..26971
                     /locus_tag="KR250_001207"
     CDS             26693..26971
                     /locus_tag="KR250_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HBH0067171.1"
                     /translation="MRNYELVYVVKPNSDEEVREAILNKVKEVVATDGEIVKVDTWGT
                     KKLAYPIAKFTEGFYVLVNFKSAVDVPKEIDRNLKINENVIRHMIVVA"
     gene            27008..27442
                     /gene="ssb"
                     /locus_tag="KR250_001208"
     CDS             27008..27442
                     /gene="ssb"
                     /locus_tag="KR250_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBH0067172.1"
                     /translation="MNQVVLVGRLTRDPELRYIPGTGTPVASFTIAIDRDYVKKDGSK
                     ETDFIPVEVMGKSAEFCANYITKGRLVALQGSIRVDNYQTQSGEKRTFTKVSTRSVQA
                     LDSKNKSENSYRESAPAFEPSFEPQGLDPQGFQAIDDDDIPF"
     gene            27461..27688
                     /locus_tag="KR250_001209"
     CDS             27461..27688
                     /locus_tag="KR250_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HBH0067173.1"
                     /translation="MMNKKRRKKKRVCQFCADKNAKIDYKSTQRLQKYITERGKILPR
                     RISGTCAKHQRELTVAIKRARNIALLPYTLD"
     gene            27831..28151
                     /locus_tag="KR250_001210"
     CDS             27831..28151
                     /locus_tag="KR250_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HBH0067174.1"
                     /translation="MKIDKGSEITRNIKIIEWMKTEILMSVSDLFNLLFKGVKPLDEA
                     IQDTLANIIMITYLLAKRLGISFRDVDYKVKEKIKIGIDEDHSVERWYGDLSNLKKHM
                     DNRE"
     gene            28159..29115
                     /locus_tag="KR250_001211"
     CDS             28159..29115
                     /locus_tag="KR250_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YybS family protein"
                     /protein_id="HBH0067175.1"
                     /translation="MNNKIRLSKAMSIIVLAIIIALVIAYIPMLGIFSMLAAVPYVII
                     GAITDRKYSLISILVTFCVLILFADISYALNICIMYSLPGIVIGKMLKRSFEQEDSNK
                     FEPIYGGTIIFVLSMLVYFFVLKTFLNVNLLDEVSKMISEIVNIQKNSFSATELKVFD
                     KMKPDEIVSYFTNMLPMMLFLQGLLSAFVTYYLSVFFIKRITKMNIRFPKFADFYLPG
                     NAILTTFLLYLLVLFIQIIGSKLYTELIMTNLQLVFNLMFVIQGIAVCIYFLKKWIRQ
                     GPNKIMFFSGIILCLFGFMGISFVGMVDSIIDFRKVRSYKST"
     gene            29126..31120
                     /locus_tag="KR250_001212"
     CDS             29126..31120
                     /locus_tag="KR250_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HBH0067176.1"
                     /translation="MSNKQTFKLNMPEINLYIIVIGISSIILLYYNLYVGCLFFCIFV
                     YMVFHNWRTTNIRRHEWTEYIQNLSLDIDETTKKAIINLPIPLCILEFDGNISWYNGK
                     FYDMIGQKDLLDKNIEDIVKNLNLRKVLNENKEMYTEINYKEKEYTIIYNVIKNDQEK
                     NPKYLMILYWIDKTEYLKVKQNYDDEKNAMMLIQVDGYDEVLKSAAEDKRALINVEVE
                     KILSALELNSNGALRRTSKDKFFLVMHKKELKKLEAEKFSILDTIRHIDYGNNLPVTI
                     SIGIGIDGDTLNENLKLATGALDLALGRGGDQAVVKTKDKFVFYGGKSKAVEKKTKVK
                     SRLIGHALREVIQQSDQVYIMGHKYPDMDAMGAAVGVYDICKSCNKTANIVLQSVNES
                     IEIFINKINENNYYKKLFIGKEEAIDNCTKNTLVVVVDTHRPNYTECEELLKLSEKVV
                     VIDHHRRGVEFINDAVLLFHEIYVSSTCEMVTELVQYMDEDVTINKLTAEGLLAGISL
                     DTKNFAFKTGVRTFEAASYLRKVGADTIEVKKFFNSDVKDFIIKAEIIQSTKIINNRI
                     CLAYSSTEIDSINVIIAQTADELLNIKEVEASFVLGEKDDTIFISARSLGQINVHVLM
                     EKLGGGGHIDIAGAQLKNVSLKEAYKMVNKIIEEYLEEEE"
     gene            31121..31570
                     /locus_tag="KR250_001213"
     CDS             31121..31570
                     /locus_tag="KR250_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HBH0067177.1"
                     /translation="MKVILLKDVKGTGKKGEMKEVSDGYARNFLFPKKMAVQADSVAI
                     KELNEKNKSKEIKAQKEYEEAVLLGKQMEEINIEIYSKAGEGGRLFGSITSKEIAEQL
                     KKQKDIDVDKRKILLDEPIRSLGSTFVEIKIHQKVTTKIRVDVKEKQ"
     gene            31589..32917
                     /gene="dnaB"
                     /locus_tag="KR250_001214"
     CDS             31589..32917
                     /gene="dnaB"
                     /locus_tag="KR250_001214"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HBH0067178.1"
                     /translation="MEDMTRIPPHSVESEQSILGSILLDKDAIITVTETIKPDDFYKE
                     AHKIIYECMITLSNKGEPIDLITLTEELRKQGHLNDIGGISYITSLSTIVPTTSNVKY
                     YADIVKEKSVLRKLIKASNEIINLGYSGATKIEDVLEQAEKSIFDISQEKTSDDFKSI
                     NLVLMDAYDMIEKLYTNKSDVTGITTGFKDLNKKINGLQRTDLILIAARPAMGKTAFS
                     LNLVQNAALKGDASVAVFSLEMSKEQLVQRMLSSQSSVELKKIKTGTLNDNDWPRIID
                     AMAVLSDAKIHIDDTPGIKISELRSKCRKLKIEKGLDLVLIDYLQLMEGEGNNESRQQ
                     EISKISRSLKILAKELNCPVVALSQLSRAPEQRADHRPMLSDLRESGAIEQDADIVMF
                     LYRDEYYHADSESKNIGEVIIAKNRHGETGSVELVWLGEVQRFGDKLRDL"
     gene            33122..33430
                     /locus_tag="KR250_001215"
     CDS             33122..33430
                     /locus_tag="KR250_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0067179.1"
                     /translation="MEKISLKYIYPNIIKVLDEINLFRVIDNNLRESIVVYANNVDNQ
                     YHINMTNTNFGNIINICKLEKLLDVDKFMEKVIKYEKEIIEKEEFSKIEEYMLNIGEY
                     "
     gene            33512..34801
                     /locus_tag="KR250_001216"
     CDS             33512..34801
                     /locus_tag="KR250_001216"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HBH0067180.1"
                     /translation="MKTVAIVGSQWGDEGKGKVIDYLATQADVVVRGQGGNNAGHTLV
                     VEGKKYALHLIPSGVLNPNTVNIIGNGIVFDPKGFLEELEMFKTDNISTENIKISDRA
                     HVIFPYHKELDALSEEARGDLKIGTTKKGIGPCYMDKTERSGIRICDLMDKDKFAIKL
                     KAQIDAKNEIVKNIYGKEELFDFETIYNEYLGYAEQIRKYVADTSVIVYDAVRAGKKV
                     LFEGAQGTLLDLDLGTYPFVTSSHPTSGGFAIGAGIGPNMIKDVVGIVKAYTTRVGEG
                     PFVTEQINETGDKIREQGHEFGVTTGRPRRCGWFDAVIVKYAARVNGLTSISFMLLDV
                     LTGFDKIKVCTSYKMGDKIITDFPASLDDLAKCEPVYEELDGWNEDITQIDNFDDLPE
                     NAKKYVAKIEELVGVSVDMVSVGPNRAQTIIRRNIFA"
     gene            35127..35202
                     /locus_tag="KR250_001217"
     tRNA            35127..35202
                     /locus_tag="KR250_001217"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35160..35162,aa:Lys,seq:ttt)
     gene            35209..35283
                     /locus_tag="KR250_001218"
     tRNA            35209..35283
                     /locus_tag="KR250_001218"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35242..35244,aa:Glu,seq:ttc)
     gene            35288..35363
                     /locus_tag="KR250_001219"
     tRNA            35288..35363
                     /locus_tag="KR250_001219"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35321..35323,aa:Val,seq:tac)
     gene            35397..35471
                     /locus_tag="KR250_001220"
     tRNA            35397..35471
                     /locus_tag="KR250_001220"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35429..35431,aa:Thr,seq:tgt)
     gene            complement(35854..36846)
                     /locus_tag="KR250_001221"
     CDS             complement(35854..36846)
                     /locus_tag="KR250_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBH0067181.1"
                     /translation="MNEDKIRKILAKYAKRRDDNELLLEHRKNEVYNRIPEIKSIDDE
                     ISKIGLSLAKIVLLNPKSKDEIVKKTKENIESLKVKKERLLAESNIPLDYLEIKFQCI
                     SCKDKGFLPNGEKCSCLKQEIVNEAYKMSNLDRILSQENFSNFNLNIFSPKKGSDGEI
                     SPRENMLNNLSICENFVHDFKKDNSENLLFYGSTGLGKTYMCNCIAKELLDKGNVVIY
                     QTSFRILDILEDYKFRRDTNNQISEDNYKNLFDCDLLIIDDLGTELNNSFTSGEIFNI
                     VNTRLVAGKKIIISTNLTPSQIGNTYTQRTLSRILDKFRILEFTGDDLRWERFK"
     gene            complement(36855..37913)
                     /locus_tag="KR250_001222"
     CDS             complement(36855..37913)
                     /locus_tag="KR250_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HBH0067182.1"
                     /translation="MFFKESNEIDLGEITIPNIFIDIFMPMADGLYVKVYLLGYRQAC
                     DITSNPKFDNNSIAKNLNIPLSDVLSAWKFWEEKKIIKIHDNEEYDNFNYSIEFLDLK
                     NFYIENILSNNSSIKSNTDKVVSTSENPSIRKMFNSINKIVGRYLDPSEKMSIMDIMN
                     KYNMSPDMILCAYEYVKDKTGTSKPVKYIEGIIRNWYDSNLYTPKDVEESFLVRSERY
                     ILYKTIFNELGFSRQPSKSEKELMDTWFDKFNMDIDLIINACSKSKNISNPSISYING
                     IIKNWNEKNIKNLNDLKQKEEERIVKENINKKQINTTQNNNTYKKTKFHNFNETFTQY
                     TSDELDEIIKKSQKEKFK"
     gene            38038..39501
                     /locus_tag="KR250_001223"
     CDS             38038..39501
                     /locus_tag="KR250_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HBH0067183.1"
                     /translation="MVIKFNQKVRRTLSVAILFMVLIIGCFIEKDDSLETYKEKTNSK
                     INQYNMDVIFDDETKRLMCNQNVDYINNTKSNIDKIYFHIYPNAFSKKDFAPFEKSEM
                     TRAYPNGFNEGYIDIKNILNNNSKMEYKIKGDKNDILEIELGKELKPNERMSLDIKYN
                     VKIPNSIGRFGYGENTINVTNWFPIACVNDERGWNLKSYETIGDPFYSETSDFHVKLL
                     IPNKYKIAHTGKLIDDKHDNKKMLYEIEAENVRDFAFILSSKFDVNKVDSKGVAINTY
                     NLNKKLSKKATEVAKDSINIFSELFGKYPYKEFSVVASDFFIGGMEYPMLVMIDQSLY
                     NEKNEFLLEYVIAHETAHQWWYSAVGNDEISEPWLDEALTEYSTIVYFEQKYGKDMAN
                     KLIKTMEIQTKSYLSENIFKPANEYKNSTEYSLNVYTKGAIAFNEIRKEVGDKVFFET
                     LNEYYNKYKHKNANGRAFVELWNSKGVDINKIISECK"
     gene            39603..40400
                     /locus_tag="KR250_001224"
     CDS             39603..40400
                     /locus_tag="KR250_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBH0067184.1"
                     /translation="MLKYCSVGSGSSGNCHYIGYKNTNILVDAGLSGKRITTGLKDIG
                     VDADKLKGIFITHEHVDHIKGAGILSRKFDIPVFANIKTWCSMKDKLGDIKDKNMKVF
                     ENDKTYSLGDIIVRPFSIEHDASDPVGYNFYTESDEKMSIATDIGCITQNIKKHLYKS
                     KLVVLESNYDPNMLMMGSYTYALKKRVMSSTGHLSNEDAANFCVELINEGTESILLAH
                     LSKENNFPELAYETSKGVLASNDIVVGQDVKLDVLSRNDVSNVYEMK"
     gene            40413..42515
                     /locus_tag="KR250_001225"
     CDS             40413..42515
                     /locus_tag="KR250_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC/EAL
                     domain-containing protein"
                     /protein_id="HBH0067185.1"
                     /translation="MLKRNFENVEDNKILYSIKQGMILAIPAIMTGSTALVILNLPIK
                     AYQEYLSGLFNGEVTNILNFINDSTLGIISLIILLTISYSYGKIYGSKYTVLVPIVAM
                     CSFLVFSHGNELNSYIEIFKTKWLFTSIIVSMTSSVLFVKLTESFVTSVKFHTEGADA
                     DFNMVVSAIVPFIIVVFLFSIFRVVMISLIGSSNFQDIFYNLFSNVFNKMGTNLMSAL
                     LFIFLMHFMWFFGIHGSNVLDTVAKNLFENSMAININLVNNNQLPTEIFTKTFFDTMV
                     LLGGCGSLLCLVIAIFLSEKRINVRKLAKIASIPALFNINEMLVFGIPIVFNYIMFVP
                     FVITPIILTVTSYVAMSTGIVPCTVSAVEWTSPIFLSGYMSTGSIRGSLLQVFNLCVG
                     VMIYIPFIKMSQNRYTYMFKNNIENLTNLLKRYELTGEQPNLLNHNGKIGSISKMLAS
                     DLKFAMKRGEIELFYQPQVNYNGNVVGAEGLLRWKHSIGGFIYPPLVIILAKEEGFLD
                     ELGEYIINKACIDLKKSEKLLRNPVKFSVNISPDQLDDPKLPEQIKNIISSNDINPNM
                     LGIEITEQIALSGSQIIIERINAIHNLGIKLIMDDFGMGHSSLIYLQNNNFDIVKLDG
                     SLVKEILTNKRSSEIIMTIVNLSKNLDFDIVVEYVENIDQRDKLYGLGCEIYQGYYYS
                     KAIPFEEFVEYANQEREI"
     gene            42583..43062
                     /gene="rlmH"
                     /locus_tag="KR250_001226"
     CDS             42583..43062
                     /gene="rlmH"
                     /locus_tag="KR250_001226"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HBH0067186.1"
                     /translation="MNISIVVVGKIKEKYLKLGIDEFKKRLSKYCKLEIIELDDEKAP
                     ENLSVKEMEIIKDKEGKKILGKIKHNSYVIALAIDGKGLSSEELAKTMSDLAVRGNSS
                     LCFIIGGSLGLSDEVLGRADYRLSFSKMTFPHQMMRLILLEQIYRAYRINNGEPYHK"
     gene            43211..43522
                     /locus_tag="KR250_001227"
     CDS             43211..43522
                     /locus_tag="KR250_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBH0067187.1"
                     /translation="MIKIMLACSAGMSTSLLVTKMESAAKENGIESQIWAIPESTIQN
                     EIEKCDVLLLGPQVRYVLPKAQEIAKPYNIPVEVINMMHYGTVNGEAVLNRAIELNNT
                     K"
     gene            43570..43887
                     /locus_tag="KR250_001228"
     CDS             43570..43887
                     /locus_tag="KR250_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBH0067188.1"
                     /translation="MDEKTIEISFNIIGYAGEGKGLAFEAIKEAKNGNIEKAKELLKE
                     SREVINKAHRYQTELIQNEASGNRTEISVILIHAQDHLMNGMNFQQLAEEIVDLHLKL
                     QDK"
     gene            44100..44801
                     /locus_tag="KR250_001229"
     CDS             44100..44801
                     /locus_tag="KR250_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBH0067189.1"
                     /translation="MLRNQPLYIQVVDILKQKILNNEYKIDENIPTERELEQEFGVSK
                     ITIRKAIEILESEGYVEKKSGKGTKVISNSLFNKLSKAESFSSILLSKGHKLSKKTIS
                     IEKIINDTNSQVFSIFGEKCSKITRMYYLDSKPYIFFTHYIPFDENLTVESFNNESSL
                     YMYLYRRNINITRFEDEFFIEESELEVSKALNLAKDILLGRKRKTYDENDNIVELSFA
                     RYNTEVQNYVIKYEI"
     gene            45025..46326
                     /locus_tag="KR250_001230"
     CDS             45025..46326
                     /locus_tag="KR250_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBH0067190.1"
                     /translation="MEKFASFLERKIMPVAAKISNQRHMRAVRAGIIATLPLTIVGSF
                     FTILLNIPIDAYMDFIAPFKAILDIPFRYTVGFLSLYATFGIASSLAKSYDLDTTGVG
                     MLAVMAFLVSTIVPTQVVEPVGGVIEAGRWMDMAKLNSPSLFGAIVTSLITVEIYRFM
                     KEKNITIKMPAGVPVEVSNSFTALLPTAVILMLFWVIRHILGFDISVALSAMLSPLKG
                     FLSGNSLGGGLLTVFLITLFWVLGIHGPAIMGPVIRPIWDMSIAENITAFTEGVSAHA
                     MPNIFTEQFLQWYVWIGGAGATLALVVMFMFSKSQYLKSLGRLGFLPGLFNINEPIIF
                     GAPIVMNPILGIPFIIGPIIMTILSYVLTITGVIPMMVARLPFAMPAPIAAVMSTNWS
                     ILAGILVLINFVISFAVYYPFFKVFEKQQLQREQEEMSENL"
     gene            46463..47557
                     /locus_tag="KR250_001231"
     CDS             46463..47557
                     /locus_tag="KR250_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HBH0067191.1"
                     /translation="MERRLGISVYPEHSNMEDDKAYIKMASKYGFKRIFMCMLSITRP
                     KEEVKKHFKEIITYARELGFEVILDIAPNIFDILQISYDDLTFFSELNASGIRLDYGY
                     DGSKEAMISFNPYDLKVELNMSNDVAYLDNIITYKPNVNNIYGCHNFYPQRGTGLPYD
                     FFVRCSNRFKKYGIRTAAFVNSKYATIGPWNINDGLCTLEMHRNLDIATATKHLFATG
                     LIDDVIVGNAYASEDELKRMSEVNKYQVVFNVVTEEKTSEVEKTIMANEQHFRRGDIT
                     NQVARSTEVRKKYAKENFPKHDNTTIFKRGDVVIGNDDFGKYKGELQLILQDCADERK
                     NKVGRIKEDEIMLLDFIEPWSKFKLILEGE"
     gene            47559..48692
                     /locus_tag="KR250_001232"
     CDS             47559..48692
                     /locus_tag="KR250_001232"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HBH0067192.1"
                     /translation="MKIDILIKGGKTVDKSIIDIAILDDKIIEVKNQIDDSKYQANQI
                     INLDGEKFISAGWIDIHTHCYERLDLYKDYPDEVGIKSGVTTVVDAGTTGALDIGKFY
                     EDTKKYKTNVYALINIAKQGITSQDELSSMMNIDEYELKRAVKKYKDFVVGIKARMSK
                     SVVISNDVEPLKVAKRIKNELNLPMMVHFGSSPPTIEDIFDYMEKGDILTHIYNGKPN
                     GILRGNEVKKEIIEARERGIILDVGHGTESFSMDIAMKSKDAGIFPDTISTDIYIKNR
                     INGPVYNLSTTMEKFIYMGYSLEDIIDKVTKNAADAISLKNKGLIKEGYDADLTIFDV
                     VNEEKELQDSLNKSVITSTSIKPRAVVVNGEYLNIGKSIGENE"
     gene            48710..49819
                     /locus_tag="KR250_001233"
     CDS             48710..49819
                     /locus_tag="KR250_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgaE family pyridoxal phosphate-dependent
                     ammonia lyase"
                     /protein_id="HBH0067193.1"
                     /translation="MSSIFEKYSVRRVINASGKMTILGVSTVDKEVIEAIGEGCKNFF
                     IIEELINKTGEYIANLLGCESALIVSSASAGIAQSVGAMIAKDDMSLVYNVNNPKKQV
                     KREIIIPKGHNVDYGVPVEVMIRLGGGEVVEAGYANMCNKEHIIREINENTAAIMYIK
                     SHHCVQKSMLSVLDAVQVAKEYNLPLIVDAAAEEDLNVYYELGADLVIYSGAKAIEGP
                     SSGLVIGKKKYIDNVKLQSKGIGRAMKIGKENIVGLTCAIERYISNDKVTLKEMEDKL
                     NPFIDKINTIKGVSASITRDSAGREILRGEIDFNEDIINKSTQQIINELRSGEIAIYT
                     RDYKANEGKIEIDIRSVTGSDLDTIYNRIKNIVES"
     gene            49884..50624
                     /locus_tag="KR250_001234"
     CDS             49884..50624
                     /locus_tag="KR250_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KDGP aldolase family protein"
                     /protein_id="HBH0067194.1"
                     /translation="MNCIPQYYKNRVCLNVLAGSIKNAKEVYDACDGYVLIGVLSKNY
                     STVEAAIEDMKKYSKEIDNAISVGLGAGDPNQSNMVSQISKELQPQHINQVFTGVATS
                     RALLGQNDSIINGLVSPTGKPGFVKISTGPLSSKEKDGIVPIETAIAMMKDMGGSSIK
                     FFPMGGLKTKDEYIEVAKACAKHNFYLEPTGGIDLDNFKEIVQIALDAGVEKVIPHVY
                     TSIIDKETGETKVEDIGVLYKIMKELLG"
     gene            50629..51621
                     /locus_tag="KR250_001235"
     CDS             50629..51621
                     /locus_tag="KR250_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HBH0067195.1"
                     /translation="MNILAFGEIMMRLSVPDYKFLTQTNELNYIITGTGLNILSGLKN
                     FGYNTYMLTKLPNNNVGKASSANIRKLGVKDDFITYGGNHIGVYFLENGYGERPSEVT
                     YLNRLNSSFCESKLQDYDIDKCLEEMDIVLICGIAMQLTDSIKEIALELARKSYEKGI
                     KVFFDFNFRQSLNPERTYDDLVNDYKKILPYCHGLFASYRDINKILNIDSLNIKSSSE
                     LIKKEYDIGIVAGTMRRFDDMNNRFIKGYISNDDGYFESREYKLEIYDRVGAGDGYVA
                     GIIYSYLENMKSEDIVEFGVASSVLAHTTYGDNSLVSTGHVLRLMNNEKIDILR"
     gene            complement(51809..52690)
                     /locus_tag="KR250_001236"
     CDS             complement(51809..52690)
                     /locus_tag="KR250_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBH0067196.1"
                     /translation="MNTKQLEYFISVAENLSFTKTAEKFYISQTAVTQQIKALEEQIN
                     VTLFTRSKRHVELTPAGKVFLSEARTIIKNINDAIAKTQQFAHGFFGTLSIGTLIGYE
                     KNKLQQYLKEFSNTYPNISMDICTNEITELLNLVKNNSMDLAFVINPENQPLKDFEYK
                     TVERYSLVALLPPGHPLYNEDSIDLIELQHDKFIFVKETGDEYGQKSMIQNRYREAGF
                     VPNVVQRSNNLNTIESLVSSNMGISILPSFCVTDTMIERDIAIVPIKEVENRIEVVVV
                     WNKKNTNPALEKFISII"
     gene            52963..54351
                     /locus_tag="KR250_001237"
     CDS             52963..54351
                     /locus_tag="KR250_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0067197.1"
                     /translation="MEDKFYAKGNGNNGYIKNLEVCSFNNLDGTCGMFQMALYKRDEK
                     YYLYGCCFGGNKKNGVMISDITDPYNPQFIKHFQMLDPKEYPTTTTPKIQIADDLMIV
                     AMSCGSGPGALVDQAKLANIKCEAGIRIYSLKEDPLNPKFLGYWDCGLKHVMGVHRFM
                     YNGGRYVHLSSDCVGFEGLIYRVIDIINPTNPVEIGKWWRPDQYADGYPNRTFDAGAP
                     HCPEFMDKGWLHGPPFVRDGKAYCGYGGAGLVVLDVEDLTRPRCLGELPFMPAFSSRL
                     AGARTHTALPLPGRDLVVVQNEGERFQFFKPDNITDVQAMNNIHMVDVSDPTKPTLIA
                     QFPYPEVPKDFPYPNFNVAGLGKPGPFGPHNLHEPMDNKPWLEQRGDRVYCCYFHAGL
                     RVYDVSDPYYIKELAYFIPPNPNKTPEESYFPGFPGPRLAVTEDLIVDDRGYIIIDAL
                     DDGFYILKMKED"
     gene            54379..55332
                     /locus_tag="KR250_001238"
     CDS             54379..55332
                     /locus_tag="KR250_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBH0067198.1"
                     /translation="MKILGISFGTKNGNNDTMCKVALKGAKEAGAEVEFIQMSSINIK
                     HCTGCCACVKTLLSGKGSMCVLKDDFEWLLDKMKDADGIVVSDPIFEEGASGLFHTIM
                     DRFGPRADRGNNIIGTKVAEAIGGKIPDSRMLNDKVISFMGIGGSDWGTRVQCEHAML
                     ALIPMWKVIDNAWFPWAKELVMDDTRLVQVHQIGLNIVEAAKDFEKASYQGEEGVCPH
                     CHNRLFYLEPGTKKAICALCGIVGELDTISGKTIFSFPEEQLGHAHDTLPGKFIHGDD
                     IKKMEGHYAEVRKTQEFKERKTSYNFIETLTKEKQAGVSLG"
     gene            55392..55616
                     /locus_tag="KR250_001239"
     CDS             55392..55616
                     /locus_tag="KR250_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0067199.1"
                     /translation="MNEKPSFKQIEVILAVIFLVLTYYKVRLTPAFIVIFIPLYLSYF
                     AVSMIVNQIAIKLLEIRQAREATNYNIGNG"
     gene            55609..56121
                     /locus_tag="KR250_001240"
     CDS             55609..56121
                     /locus_tag="KR250_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HBH0067200.1"
                     /translation="MDRKKKEILTLSMGIALLPPLWAVLAPYIGIKTGAVALICAGLY
                     VTNGNKQEDGLKIMFGFWCGDLWAVLAILIMGYMNFNQNLELFLTLSILGFFAVVIAS
                     LFEKIIFLPSWLCGWAIGLTIMTGENIINLQDICIQIAVAMAVGVWYVGAGVDLFTIY
                     ILSKDRKKGN"
     gene            56153..57007
                     /locus_tag="KR250_001241"
     CDS             56153..57007
                     /locus_tag="KR250_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase"
                     /protein_id="HBH0067201.1"
                     /translation="MAMGFEFKIMRSLIYVGLAKEEYRPKLMDWLYRHHIPDSISTFG
                     PYCTKYAFYQAYPTPNEGERFGARKMQLTEHYWLVDEHMPEMANRIMTEYMPMDVLRW
                     QGCIPDVENKRVHENAESGDAGRAVGGDNGCPPFIFAFVPINWEEDFRGKGRTVQDGP
                     NYRWQFMIKYPDGISKEEGEKWFYDEVVPYFTNCCYVNRFVSSKIMINYGATAFDRVS
                     ELWFEGEEEWYKAVVEETKSFIKKPEWAQEEEFPYLKPQFNIASVFLGDIATMDAYSQ
                     YRGYIPMR"
     gene            57541..58335
                     /locus_tag="KR250_001242"
     CDS             57541..58335
                     /locus_tag="KR250_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBH0067202.1"
                     /translation="MYTIGQVSKFLGISRDTLKFYEDKGLVNPKKNDENGYRIYNQVD
                     IYDIATINFYREIDIEIKKIQEIRKSKSINNLELLLEEKEQIILKEIEYKKLLLKKVQ
                     LVKEDCDKIKTYFGKYTIKEMKPLEVKGKIKHVTNYEEYEALKENTNNLKKAVTLTDL
                     RRVINFNEEGIIEEKFIIVREVDDYNKIIEGEVLSHPNCIYTIIEDGRWSTGGENIDN
                     KVEDRLREIAIENECELLGIVYVNILLTTYEEGIERIFLEIYAPIK"
     gene            58439..>58773
                     /locus_tag="KR250_001243"
     CDS             58439..>58773
                     /locus_tag="KR250_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBH0067203.1"
                     /translation="MRTEKSLKRPIASFIILTNIIFLPLFLLVVVTIMLKLPTVISDI
                     ALCISAWSSTFAFMILFKKIYPGKKFLVHVKDRFRNKLKFSTVSIIISIQVLIAVVVI
                     FILASKNHG"
BASE COUNT        22320 a         7154 c         9757 g        19542 t
ORIGIN      
        1 atataaatta acctactgtt taattttcaa tgttcgtgtt tatttcttgt tccttttttc
       61 aaggacaagt aatactatac catctttaaa acagtaggtc aatacttttt ttatattttt
      121 ttataaattt ttttcatatt cttttttcag cttctctact acctttttct ctagtctaga
      181 aacagtcatc tgtgatatcc ctagttcttt agctatacta gattgtgttt tatcaaagaa
      241 aaatctatct ttaaaaattt taacttctaa ttcatttaaa gtttcaataa acttattaat
      301 aaaatcttca tactctatac ttccaaagtt accttcttct tttccaatct tatcaataag
      361 agttatatct ttatcctctg aatcatcatt acttgatgaa tccaaagaca ttggttgata
      421 tccataagaa gcctccatag cctctaaaac atcttcttca ctaacgccta tataatcagc
      481 tatatctttt acctttggat gcttcttatt ttcttgttct aatttaatct tcgcatcact
      541 tatctttttg cttaattcct gtattcttct tgggactcta agtgtccata ctttatctct
      601 aaaatacttt tttatttctc ctactatagt tggtgttgca aagctggaga actcaaatcc
      661 tttctcaaca tcatatctat caatagcata aattaatgcc aatgaagcaa cttgatatat
      721 atcttcaaaa tcaacacctt tgtttatata cttcttagct aacaatcttg ctaaatacag
      781 atgtctttca ataagaatat ttctaatgtc tacattttta tttttattat ataaattaaa
      841 tagctctttt gtatccatat ttaggtaatg tgtagcatta gctacatttt tcattaaata
      901 tcaactccta aatatttagt catttttatt tttgtccctt gattatgttc tgattcaata
      961 tctacatcat ccattaaagt tttaattata aacaatccaa gaccactctc ttttggattc
     1021 tctaaatctg gtgtacttat tgagctaaca tcatatcctt ttccattatc ttgaatttct
     1081 atattaattg cattatctaa aatattaaat actatattaa atacatcatc tttactgtgt
     1141 tttattgcat ttgtacatgc ttcagatact gctaccttta tatcttctat atcatctatt
     1201 ggaaaaccca tcttattggc aattccagat gctgttaatc ttattatact aacataatct
     1261 ggatttgtag taatttccat ctttatagtc tcacaagcca aagtttatcc ctccacttta
     1321 aatattttat ctagacctgt tatagtaaaa atctttctta cgttactctt aggatttact
     1381 atgtatatct ctttttcatt tatttttaat ttttttaata cacctatcat agctcctaag
     1441 cctgttgagt ctatataatc taaatcttta agattaatct ttacatctaa catatttttt
     1501 tctattaaag catgtaaatg ttcctttagt ttatctgctg tagaaacatc aagttctcca
     1561 tcaagacaaa cattccaaaa tttattttgt gaatctaaat ttgaatcaat attcatagac
     1621 atgtaaaaac ctcctatttc cttttaataa ttttaaatat atttcctata ctcaatattt
     1681 tattaattac ataaactata tcatctattc cctttgatat tgatgatgca ttttcaacaa
     1741 tatcttgtat atgtctttca ttataatcta caatcttata tgccttttca acaaccttat
     1801 ttatgctgtt tagagtttta gctaaataaa ttgctactat aagagcagag cttcctacta
     1861 acactgctcc aatttgccac ccccatgcat tcattagtta acatctccct catcatcaaa
     1921 gcttttactt attactatat cttcttctga aatctttata tcttcttcat tattatcatc
     1981 atccactaaa tttattattc tctctttaac atcattaaat gtttcatgta gaacttcttt
     2041 tggattttct ttaacatcat taaacttctc ttttgtttct tttcttagtt cagaaccttt
     2101 tttaggtgca aaaaacatac caaatataaa ccctaaaata gctcctaata ataaaaatcc
     2161 acctttttta caacttcttt tattgcacat aacaattact cctttcaatt ttaaattttt
     2221 atctaactac attatacaaa aagaatagga tatttatcct attcttcttc aatatttatt
     2281 ttggcaatcg aaactacatt gacttcatca ttcgtcttca ttaatttaac accacttgta
     2341 actcttccaa acagtgatat ttcattgact cttattctaa taagaactcc atcagaattt
     2401 ataatcatta tctcatcatc ttcgttgacc atatctgcac ctacaattgt accagttttt
     2461 tcagatatat tataagtttt aattccttta ccagctctta tttgacttct atactcttct
     2521 atatttgttc tctttccaaa accattctta cttacaacta acacatctgt acctttactg
     2581 caaaggttca tagacacaac aaaatcttct ttacttaaag ttataccttt tacacccatt
     2641 gctgttctac ccatatatct tatatcattt tcatcaaatc ttatagacat accttcttta
     2701 gttactaaaa gtacttcttg ttttccatct gtaagttcta ctccaataag ctcatcatca
     2761 tctcttaaac ctatagcaat aagaccagat ttatttatat ttttgaactc ttctctctta
     2821 gtctttttaa caataccttt tttcgttgca agtaataaat attcattttc atcattacca
     2881 tcaataggta ttagagtagc aattttctca tctgctgaca attgaagtaa attcactata
     2941 gcagtacctt tagcctgtct cttaccttct ggtatttcat atgcatttaa tttaaatact
     3001 ctacctttat ttgtaaagaa taatagtcta ctatgagtag ttgtagttac caagtgcctt
     3061 acaaaatctt cttctctagt tgtaagtgct gaaatacctc ttccacccct tttttgactc
     3121 ttataagtat cagatggaag cctttttata tatccaaagt gagtaagagt tattgctatt
     3181 tcttcatcac ttataagatc tctcatatct atttcgcctt cagcatgtct tatttctgtt
     3241 cttctctcat cagagtaatt ttcttttatt attgtaattt catcctttat tacatttaaa
     3301 agtaatcttt catcagctaa aatctctttt aatctattta tcttcttgat taaatcttca
     3361 tattcagctt ctatcttatc tctttctaaa cctgtaagtc tttgaagtct catatctaat
     3421 atagcttgtg cttggatttc agttaatttg aatttttcta ttaaacctag cttagcttct
     3481 tgcccagtct ttgaagctct tatcaagctt ataacagcat ctatattatc taaagcaatc
     3541 tttaatcctt ctaaaatatg tgctcttgct tcagctttat ttagttcaaa tttagttctt
     3601 cttgtaacaa catcttcttg atgtttaata taatgatata atatttgttt aagatttaaa
     3661 actcttggct gaccatctac aagtgcaagc attattatac taaaagtatc ttccatttga
     3721 gaatgtttat acaaattatt taatactata ttagcattag catccctctt taattctata
     3781 acaattctca taccatttct attactttcg tctcttaggt ctaatatacc ttctattctc
     3841 ttttctttaa ctaactctgc tattctttca accagtttag ccttatttac ttgataaggt
     3901 atttctgtaa ctattatttg ctgttttcct tttggtagct cttctataaa agctctagac
     3961 ctaactttaa cttttcctct tccagttctg tatgcttctg ctatactttc ttttcccatt
     4021 ataattgcag ctgtagggaa atctggtcct tgaacaaatt ttattaaatc atctacacta
     4081 cactctggat tatctatcaa atatacagtt gcatcaatta cttctgctaa attatgtgga
     4141 ggtattgaag ttgccatacc aacagctata ccatttgagc catttactaa taaattagga
     4201 tatctagctg gcaatactga aggctctttt aacgactcat caaagtttgg tttaaagtct
     4261 acagtttcct tttcaatatc tctcagtagt tctaatgata atttactcat tttagcttct
     4321 gtataacgca tagcagctgg tgaatcccca tcaacagaac caaagttacc atgaccatct
     4381 actaaaagtg ctctagttga aaaatcttgt gccattctta ccatagcata ataaacagca
     4441 gtatctccat gaggatggta cttacctaaa acgtccccaa caatacgagc tgacttcctg
     4501 tatggtttat ctggagttaa atttaactca ctcattgaat ataatattct tctatgaact
     4561 ggctttaaac catctctaac atcaggaaga gcacgtccag ctataacact cattgaataa
     4621 tcaatatacg attttttcat ttcttccgct atttcaatag ggagtatttt gttattttct
     4681 tccatgtata ctaatcccct ctctctatat atctaagttt ctaacaaatc ttgcattttc
     4741 ttctataaat tcttttcttg gagcaacctt atcacccata agcattgaaa atacttcatc
     4801 tgcaattgca gcatcttcta cagtaacttg taataaagtt cttgtctcag gattcatagt
     4861 tgtttcccat aattgctctg cattcatctc tccaagacct ttgtaccttt gaagttcaac
     4921 accatttctt ccaatttcat ctaataagat atttaactct ttatctgagt aaacataatg
     4981 ttccttcttt tgctttgtca ctttatataa aggtggctgt gctgcataaa catacccctc
     5041 atctataaga ggtctcatat atctaaagaa gaatgttaat aataaagttc ttatatgggc
     5101 tccgtctaca tcagcatcgg tcataattat aattttatga tacctagcct tatctatatc
     5161 aaaatcttct ccaataccac aaccataagc tgttatcata ttttttattt catctgaaga
     5221 tagtatccta tctagtcttg atttctcaac attaagtatt ttccctctta atggaagtat
     5281 agcttgacta tttctatctc taccttgttt agctgaacct cccgctgaat ccccttcgac
     5341 taagaatatt tcacttttag atggatcttt ttctgcacaa tctgcaagtt ttccaggtaa
     5401 agatgtactt tctaatacac tttttctcct tgttaattct cttgcttttt ttgcagcttc
     5461 tctagctctt tgtgctctta aagctttatc aactatgatt cttgctgttg atgggttttc
     5521 ttctaggaaa gaccccagtt catcaactgt tacactatca actatacctc tcataaagct
     5581 attacctagc tttgttttag tttgaccttc aaattgaggt tctggtaatt taactgatac
     5641 tactgctgta agaccttctc ttatgtcttc accaagtagg ttaacatcat tttcctttaa
     5701 aaatttattt ctcttagcat aatcatttac agttttagta agagctgctt taaatccact
     5761 taaatgagaa ccaccttcat gtgtatttat attgtttgca aaagaatata tgttctctgt
     5821 ataaccatct gtatattgca tagctagttc aacaaaacaa tcatcaacct ttttatctat
     5881 ataaactatg tcatcatgta taccagttct agatttattt aaatacttaa tatattctac
     5941 caaaccacct gtatagtgaa attcttttct tttttcttgt ccttctcttt tatcttcaaa
     6001 tactattttt attcctttat tcaagaaaga aagttctcta agcctatact ccaatgtttc
     6061 atatttaaac tctatttcat caaatattgt ctcatctggc ataaattgaa tcattgttcc
     6121 tttatcttga gattctccaa ctacttcaag cttagatgtt ggcaatcctt tctcatatgt
     6181 ctgcttatat ctctttccgt ttaaataaac ctctgctacc atccattttg acaatgcatt
     6241 aactacagaa acaccaactc catgaagacc tccagatact ttatatcctc cgcctccaaa
     6301 ctttcctcct gcatgaagat tagttaatac tgtttctaaa gttgatttac cagttttagg
     6361 atgtatttca acaggtatac ctcttccgtt atcttttact aaaacactgc catcttcatt
     6421 aatagataca tatatgtctg aacaatatcc ttgtaaagcc tcatctatac tattatctac
     6481 aacttcataa actaagtgat gtaaacctct agggcttgta ctaccaatat acatacctgg
     6541 tctttttcta acggcttcta atccctctaa tacttgtatc tgacttgcac cgtattcttg
     6601 tttcatttaa tttcctccat tctctatgct aataacatcg ccattttcaa tattaaatat
     6661 tgtaacattt ttttcatcaa atattttttt atgtgaaact tcagcacttg ttataaacat
     6721 ttgaacattg cttaaattat ttactaaaag tttttgtctt gcttcatcta actcactaaa
     6781 aacatcatct aaaattaata ctggatattc ttcaacttca ttttttatta attctatctc
     6841 tgataacttt aatgatattg aagcagttct ttgttgacct tgtgaaccaa aaagtcttgc
     6901 atctaaatca tttatgaata tatttaagtc atctttgtgt ataccatatc tggtagtctt
     6961 agattctata tcattaggtc tattcgatga caacttggct aaaaatttat tatatactgt
     7021 atctatagta tcttcatcag ttatatttat ttgattttta tatcgaattg ataatctttc
     7081 aactccatta gtcaaattca tatgcatatt ttcagatata ttagctatct tttttataaa
     7141 gtctctacgc aaaatataaa tataacttcc atactttgct aacgtatcat catatacatc
     7201 taataaagct tcatcaacat gtatattttt aagcacccta ctcctttgag aaagtgtttt
     7261 attataattg gtaagatatt tataatattt aggtataatc tgactaatct ctttgtctat
     7321 aaatgccctt ctttctttag gaccctcttt aaccaacctt aaatcctcag gagaaaatat
     7381 aacaacattt aagttgccaa gtagttcttg tatcttttgt aaaggaactt tatttattct
     7441 tatccctttt ttatcttttc caataattag ctctattaga ctatatttat tatattttga
     7501 aaaacttcct ccaatatata agttttcact attaaatcta accatttctt tatctttgtt
     7561 agtcctaaat gattttccaa aagaaagcat ataaatagat tctactatat ttgtctttcc
     7621 ttgaccattt ttaccaacaa gtagatttac tttcccatta aattccagat gcaacttttt
     7681 ataatttcta aaattaacaa gttgcaaact tttaagtttc acaaaaagtt ctcctttttt
     7741 acaatacttt tattttagtt ccttctattt caactatatc tcctgatttt atcttttttc
     7801 ctcttcttaa ttctatttca tcatttactg tcactaaacc ttcttgaatt aaaaattttg
     7861 catgacctcc tgttgatgca atctctgcta atttaagaaa ttgatccaac tttatatatt
     7921 ctgattctat agttatttca gtcataatag ttcctccttg tcactatttt aaacaaattc
     7981 ctattgatac actaaaagta tattatacca taaatatgct tataaaagga gttgtctaag
     8041 attagaaaaa ttttctacaa aagacaactc cttcatgtct gctatatatt tgatgatatt
     8101 cttactggaa gcagtaagta aatataatta acatcatctg ttggtttaat tatacaagga
     8161 tttacatttg tagtaaattc tataaatatt tcctcattat ctatattttt caaaccttcg
     8221 ataaaatatc tagaattgaa agctatatct aggtaatctc catctaaatc tatttcaact
     8281 tcttcataca catttccttt ttctgtatta gaagtaattg ccattacttt atctcttata
     8341 gataacttta taagattatt tttttctgat tgagacaata aagacgctct ctctatacta
     8401 tttaaaagtt ccttagtatt taatttaact ctgctgttat gttctcttgg caataatttt
     8461 ttataatcaa taaaatctcc ttcaagcaat cttgtaataa ttttcgtatc attaattata
     8521 aaaatagcat ttttttcgtt aaatccaact ttaacattat cctctccaga aagcaaacta
     8581 tttacatcta taagtgtctt tccaggaatt atgactttta tattttcagt taaactatca
     8641 accgaacaac tttttacagc taatctatat ccatcaattg ctactaaatt taagtttcta
     8701 tctacaattt ctaaaagttc tcccattaaa acaggttttg tttggtcctg tgagatagca
     8761 aatacagttt gtttaatcat attttttagg atttcttgag gtatgctata taaatcttct
     8821 tcatttaatt ctggtaattt aggaaattct tttgctgcat aacctttaat tttaaatctt
     8881 gaatttacac aatttatgta aatattattt tctgaatcag tttctatctc aacaaaggaa
     8941 tctggtaatt ttctaattat atctccaaat agccttgcat ctacaacaac atcacctttt
     9001 tctatgattt ctgcttgtac ataagtttct attcctattt cagcatcata tccagtaagt
     9061 tttaattgac cttcttctgt agatataagt atacctttta ataattctat tgttgttttt
     9121 ccattaatag ctttttgagc aattccaatt ctatttgcta ggattttttg attacaaatt
     9181 attttcaatg tggataacct cctctggctt tttatatata aaaataagtt aaaatcatag
     9241 tagtaatagt agtaggtgtt gttgataatg ttaataagta tgtataacat tataaataca
     9301 aagatattaa catgtgtata agatgttaat atctttgtta ttttagactt gataatttgt
     9361 taataatttt tgttcaaaaa aagtattaac aggttattca agagatataa acatacaatt
     9421 gtgtataaat tatcccttca aatctgatat aattttgtct attttagtct taatttcttc
     9481 actttcttct atatccttag atactttgtc atgagcatga attactgttg tatggtctct
     9541 acccccaaac tcttcaccta ttttaggaag ggataaatca gtaagctcgc gcgttaaata
     9601 catagctatc tgtcttggat aagctattga acgtgttctt tttttagaat taaaatcttc
     9661 catctttaaa ttgaatacag aagatacttt ttcttttatt ctgagaacat ttatttcttc
     9721 atttttagaa gttgttataa tatcttttaa agcttctgta gcaagatcaa aagatattac
     9781 cctattgcta agagaagaat aagcaaccac tctagttaat gcaccttcta gttcccttat
     9841 gtttgactta atattttttg ctatatatga cataacctca tttggcacat ctatacgttc
     9901 tagttgagcc ttttttctaa gtatagcaat tcttgtctca aagtcaggtg cttgaatatc
     9961 agttataagt cccatttcaa atctcgatct tagcctatct tctagtgtag gtatgtcttt
    10021 tggcggtcta tcactagata taattatttg tttgtttgct tcatgaagag tattgaatgt
    10081 atggaaaaac tcttcttgag ttctttcttt tcctgctata aattgaatat cgtcgattag
    10141 taatacatct acgtttctat atttattcct aaattcttcg tttttatcgt cttttataga
    10201 gtttataagt tcattagtaa atttttctga agaaacgtaa actacttttg agtctttttt
    10261 ttgactaaca atatgatgac ctatagcatg cattaggtga gtcttaccta atccaactcc
    10321 tccatacaaa aaaagtgggt tataagcttt agctggagat tctgcaactg caacgcatgc
    10381 agcatgtgca aatctattac tattaccaat aacgaatgta tcaaatgtgt attttggatt
    10441 tagattgggg taaagaactc tataatttaa atctgtgcta tcagaattta aatcagcaac
    10501 ctctttttcg ctcaatacaa atttaatatt atattttttt aatgacagtt gactaatagc
    10561 atcttcaatt aaatgtaaat atctattctc taagatatct ttattaaaat cactaggagc
    10621 cagtaagatt aaatcattaa gatgtatttt taatggtacg atgtttttaa aaaaggtatt
    10681 aaaacttact gaagttaagt caccttttat taattgtagg gttttgtccc ataaagaaac
    10741 tatatccata atataaacct ccgaacagaa taattaatat tttccacaaa gttattaaca
    10801 gaaaagcaaa aatatatata aattttacag aataactaaa tttatggata atggtgttga
    10861 taaattaata atagtataaa ttattaaaaa aatatgaaaa gatataaaca atatgtagat
    10921 aaagttataa ataacataaa catacaataa atggtgatta taactagagt attgtcagta
    10981 atcttaaaag ttatacacaa aagtatccac aagctgtgaa taatttctaa taagttgata
    11041 acatttatgc acattatcaa caagtatatt tataatatca aaatatatgt caaacttcaa
    11101 tgatttgtaa ctaatttatc cacaatatta actaattgtt gataaaaata taaacatgtt
    11161 aaaattactt tatttgtgta tataattgaa aaaaataatt tagaataaaa ttaaatagat
    11221 ttttactagt attttcaata aaaaaaatac ataatatata tatggtagat tttgtgatat
    11281 aagcgtccca aaaactcgtt gacaacagta gtaaaagtat ttataattat atgtgttatg
    11341 tttactttga gggtaaagtt attattttta taaacggaaa ataaaatctg aaaggcggtg
    11401 caaaataatg agtaaaagaa cttatcaacc aaaaaagaga caaagaagta aagaacatgg
    11461 tttcagaaaa agaatgaaga cgtctaacgg aagaaacgtg ttaaagagaa gaagagctaa
    11521 aggaagaaat agattaactc attaattaca aaagggccgc tgcaaaggtg gcctttttgt
    11581 gtaaataatt acaattcata gctgtgttta tggaggttaa ctatggactt taataggact
    11641 aaagggttga aaaaagactc tgattttaga aaagtatata aacacggcaa atcttttgca
    11701 aacaaatatt tagtaatata tatacttaaa aataaatcag attatagtag agtaggtatt
    11761 tctgtttcaa aaaaagtagg aaaagctatt actaggaata gagtaagaag attaataaaa
    11821 gaagcctaca gattaaatat tgatgaaaaa attaagcctg gatatgatat agtatttata
    11881 gcaagggtat ctagtaagga tgctactttt aaagacatag acaagtctat aaaaaattta
    11941 gttaaaagaa cggatatttc tatttaattg tatgctataa atatggaggt taatgacttt
    12001 gaataaaata atttatatat taaaggaaat aagtaagcat ttatcgaatt tatgtattta
    12061 tttagtgaga ttttatcaaa agtatatatc tcccttaaag ggacctacct gtagatttta
    12121 tccaacttgc tcacaatatt caatagaggc gtttaagaaa tatggatttg taaaaggtat
    12181 gtatttaacg ataagaagac ttttaaaatg tcatcctttt catccaggag gttacgaccc
    12241 tctaaaataa aagagagtaa ctattaggag gtataaaaaa attgaatata ataggaaatt
    12301 tcttaggatt gattcttaag gtaatatttg aatttgttaa tcattatggt atttctataa
    12361 tactttttac gatacttgta aagattattt tattaccatt aacaattaag cagactaagt
    12421 caacaaaggc aatgcaagat atacaacctc gtataaaaga gatacaagaa aaatataaaa
    12481 ataagccaga aaaacaaaat gaagaaattg ttaagttata tggagaagct aaaattaatc
    12541 cactatctgg ttgtttacct ttattaatac agtttcctat attaatagga ttatttagcg
    12601 ttttgagaga accagttgct catggcgttt ttgcaaacaa agctgcattt ttagcagctg
    12661 acaatgggtt tttatggata aaaagcttaa catcaccaga ttatgtatta gcagtatttt
    12721 caggagcaag tgcatatgtc atgcaaaaag taatgacacc aaaagatcag ttacaaggct
    12781 ctatgaaagt aatgacatat gtaatggcag gtatgtcttt ctattgggga tttatattcc
    12841 cagcaggact tacattgtac tggacagtaa gtaacttatt ctcaatagct caatattact
    12901 taataatgaa tcctttaaaa gctaaactgg ctgctaactc taaggaggaa aaagttaatg
    12961 agaaaaagcc taaaattaat aaaaagtaag agtaaagagg aagctataag taaagctata
    13021 gcagagttga atttaaaagc agaggatata gaagtagaaa tacttgaaaa tccaagtaaa
    13081 gggtttttag gttttatagg tgctaaagat ggtacttatg aaatttttgt tatcgagagg
    13141 gaagtagatg tagctaaaaa ttttatagag gtaatgttga aaaatgcaaa tgttgatgcc
    13201 aaagtcaatg tatctcaaaa agataattta atcatggttg atattgaagg caaagaagct
    13261 gcaagtttaa ttggtagaag aggggaaact ttagattcta ttcaattttt gactggatta
    13321 gctcttaata aaataaataa agattctcat acaagagtac ttgtagatat agaaaattat
    13381 agatcaaaac gagaagaatc tttaataaga tatgctaata aagttgctag agaagtagcg
    13441 aaaacaagaa aaactaaaaa attagattat atgaatccat atgagagaag aataatacat
    13501 tcagctcttc aaaatgacaa atatgtaatt acatatagtg aaggaaccga tccatataga
    13561 agattagtaa ttgagtgtaa aagataaagt caacctccta aacattaggg ggttttattt
    13621 tttaatatta ttaaatcatt aatggttggt taaatgtaaa tctatatgta aaaaattatg
    13681 aattaagagg tgataaattt gtttatagat gatacaattg cagcaatagc tacagcacct
    13741 ggagaaggtg gtataggtat acttagaata agtggagaaa aagctttgaa ggttgcagaa
    13801 gaaatcttta aatctatgtc tggaaagagt attgaagaat acaataagag aacattaata
    13861 tatggaaata tagtggataa tgaaaatgta attgatgaag ttttattggc atatatgaaa
    13921 ggaccaaatt cctatacagg agaagatgtt atagaaatta attgccatgg tggatttatt
    13981 tcggtaaaga agatattaga attaatacta tcaaaagatg ttagattagc agaggctggt
    14041 gagtttacta aaagagcatt cttaaatgga agaatagatt tatctcaagc agaggctgta
    14101 attgatgtta taaaagccaa aactgatata gctcatgaag ttgctcaaaa tcaattagag
    14161 ggttctttat caaagaagat aagagaattg agagataaag tcactgagat attagctcat
    14221 gtagaagttg ctatagatta tcctgaggaa gatattgagc atataacata tcaaactctt
    14281 aaagaaaaaa ctgatgaact taaaaaagac attaaaaaat tatatgatac agctgaaagt
    14341 ggaaaaatac ttagagaagg cttaaagacg gtaattgttg ggaaaccaaa tgtaggtaaa
    14401 tcttcactac ttaattctat tttaggagag aatagagcaa tagttactga tataccagga
    14461 actactagag atgttataga agagtttgta aacataaaag gtataccttt aaaaattgtt
    14521 gacacagcag gtataagaga tactgatgat attgttgaaa aaataggtgt tgaaaagtct
    14581 aaagaatcat ttactagtgc agatttgata gtaatggttt tagatgcatc tagaaagttg
    14641 tcagaagaag atatagagat attagaaaaa cttaaggata aacaaacaat agtgctgtta
    14701 aataagaatg atttgaaaca agagattgaa gaagaaaaaa tacttaaata tgtagaaaat
    14761 aatagtataa taaaaatatc agctttacaa caagaaggaa tagaagaatt gcaagataaa
    14821 atagagtcta tggtatataa aggtagtatc aaaaataatt ctagtttagt agtaacaaat
    14881 tcaagacata aagatgcact tagtaaagca tataaatctg caacagatgc tttaattgct
    14941 ttggaacaaa gtatgccatt tgactttgta gaagtcgatt taaaaaatat atgggactat
    15001 cttggatata ttaatggtga tacagtaaca gaggatttac tagataatat attccataat
    15061 ttctgtattg gtaagtaatg tacaaaagta aacttgtttt tattatagag atttttaaaa
    15121 aatttgaagt gtttctcatg aaacattaat tttgaaaaga aaggaattat aaatgataaa
    15181 gtttgaagca ggaaaatatg atgttatagt agtcggagca ggacatgctg gatgtgaagc
    15241 tgctttggct actgcaagaa tgggatataa aactttgata ataacaatgt ctttggattc
    15301 catagcatta atgccttgta atccatcaat tggagggact ggtaaaggtc aattagttaa
    15361 agaaatagat gccttaggcg gtcagatggg attaaatata gacaagacat atatacaaag
    15421 tagaatgtta aatactgcca aaggtccagc ggtacactct ttaagagcac aagcagataa
    15481 atttaaatat catgaagaaa tgaaaaagac attagaagat gaaccaaatc ttgatatagc
    15541 tatggatgaa gttgtagaaa tacttcatga aggaaatgtt gtaataggag ttggaactaa
    15601 actaggatgt tcatttaaat ctaaagctgt aatattggcc actggagttt atctgaactc
    15661 aaagatatat atgggtgaag ttgcatttta tgaaggtcct aatgcattag ggtatgcaaa
    15721 atatcttaca gatagcttag ttgagcttgg ccttagaatg agaagattta agacgggaac
    15781 tccagcaaga gttcatagag atagtataga cttctctgta atgtctttac aagagggtga
    15841 tgaaaaagta actccattct catttatgaa tgaaaatata gaaaaaaaac aagaaccttg
    15901 ttatttaaca agaactacag aagaaactca aaaagttata ctggataatt taaaaaggtc
    15961 tgctatgtat agtggagtaa tagaaagtac tggtccaaga tactgtcctt ctatagaaga
    16021 taaagtagta agatttagtg ataaaacatc acatcaatta tttatagaac cagaagggtt
    16081 aaatactaaa gaaatgtata tacaaggaat ttcaactagt ttaccttttg aagttcaatt
    16141 agatatgtac aaaactataa aaggacttga aaattgtaag ataatgagac ctgcatatgc
    16201 tattgaatat gattgtgttg acccaactca actaaaaata agtttggaaa taaagggtgt
    16261 tgagaactta tttagtgcag ggcaatttaa tggaacttct ggatacgagg aagcagcagc
    16321 tcaagggctt atggctggta taaatgcagt tagaaagata gaaggaaaag agccatttgt
    16381 attagaccgt tcagaagctt atataggtgt cttattagat gaccttgtta ctaaaggaac
    16441 taacgagcct tacagaatga tgacatcaag agcagaatat cgactatatt taagacaaga
    16501 taatgcagat atgagattaa ctcaaaaagg ttatgatata ggattagtta aaaaagatag
    16561 atatgaaagg ttcctaaata aaaaggctgc tgtagagaaa gaatttgaaa gactaaaaaa
    16621 tgaaagagtt acaccaaaag aagtaaatag tttgttggaa gaaaaaggag caacaccaat
    16681 aaaggttgga atatctctat atgaattttt aaagagacca gaggttactt acgaattgct
    16741 tgaagaatta gggaaaggcg ctggagaaga tgtttcaaga gaagttaaag aacagtgtgt
    16801 aataataact aaatatgaag gttatataga gaaacaattg aaacaaatag accaatttaa
    16861 aaaacttgag aataaaaagt tggatgagaa aataaattat tcatctatag aaggtcttag
    16921 attagaagct agacagaaat tagatgatat aaaacctatt tcaatagggc aagcatctcg
    16981 tatatctggt gtttctccag ctgatatatc agttttactt atatacctag aacaaatcag
    17041 aagaactaga ggtggcaaag gtgaataata tagaacttct gaaaaacggg attgaaggtt
    17101 ttaatataga tacgaatgac agtatgttag aaaaatttaa aatctataga gagatattag
    17161 ttgaatggaa taaaaagatg aatcttactg gaatagaaga tgaaaaagaa gtgtatataa
    17221 aacatttttt agactctatt tcagctgtaa aaaatggata tataaaaaat gggatgtcta
    17281 taatagatgt tggtacaggt gctggttttc ctggaatacc attgaaaata tgtttagata
    17341 gcttagaact tactttgtta gattcgttaa ataaaagaat aaacttttta gaggaagtta
    17401 gtagagtact agagttagat aatattacat ttatacatgg aagagctgaa gattttggta
    17461 aaaatgaaga ctatagggaa aaatatgatg ttgctacggc aagagctgtt gctggattac
    17521 cgattcttat ggaattttgt gtaccatttg taaaagttgg agggtatttt atttgcttaa
    17581 aaggtccgaa tgctaattta gagctagaag aatcacaaaa ggctattgat gttttaggat
    17641 tggagtatat tgaaaaaata gatgtagagt taccagaggt agatttaaat cataatatat
    17701 tagtttttaa aaaagtgatg gaaacaccag ttaagtaccc tagaaaagct ggtaaaccag
    17761 ccaaaaatcc tattaagtaa tgaaaaattt attattggta agcttaatta tttctcagga
    17821 aataattaag cttactgtaa aaaaacaaaa ataaaggaat tattaaagta gatgcagaaa
    17881 taaatttgta tgtacatata gagaacaaaa ataatttata tctaaaataa gagaggtatg
    17941 ttatggaaga taaaagagtt atggagatac ctatagaaga tgtagttcca aatccatatc
    18001 aaccaagaaa aatattttca caagtttcat tggaggaatt gagcaattct ataaaggtat
    18061 atggtataat tcaacctata acagttagag caaaagatgg caaatatgaa ctaattgcag
    18121 gagaaagaag attaagagca gctaaattag cagagcttaa aaccatacca gcaattataa
    18181 ataatatgaa cgatgaatct tctgctgttc tagcgttatt agaaaatttg caacgagaag
    18241 acttaaattt cattgaagaa gcaattggtt atgaaaactt aataaaagaa catgctttta
    18301 ctcaacagca attagcagaa aaattgggca aaaatcaatc tactgtagca aataagctta
    18361 gaatattaaa gcttccaaac gatataaaaa tgaagctaat agagaataat ttaacagaaa
    18421 gacatgctag agcatttctt aaactaccaa gtgaagattt aatgcaaagt gtgttagaca
    18481 aagtcataaa aaatgaattg actgttaaaa aaacagaaaa attgatacaa gatgtattag
    18541 aagaaactaa agttcaagaa gagcctgata aaaaacagaa tataaagggt gctatgagta
    18601 taagaattta cataaatact ataaagcagg cctttgatgc tatatcaaat acaggaattg
    18661 atgctaagta taatgaaata gataagggag attatatgga agttgttgta aaaatcccta
    18721 aaaaatagag attactgctt accagtaatc tttttttgta atataagcct tgtaatataa
    18781 attgatttat agacattatt tatacttaaa aaacacctat tcaacattta aaattaattt
    18841 aattcttaaa ggggggaaca tcatgggcaa agttatagct gtattcaatc aaaagggtgg
    18901 agtcgggaaa actacaacaa atgtaaattt aagtgctagc ttaggaactt tgggaaagaa
    18961 aatattagta ttagatttag acccacaagg aaatacaact agtggatatg gtattaataa
    19021 aaacgaagta gaaaatacaa tctatgaaat tatgttagat gggttacata ttaaagaagc
    19081 aataataagt actgaatttg agaatgtaga tgtagttcca tcagctactg aactatcagg
    19141 tgcagagata gagcttacat ctaaaactaa tagagagtat attttaaaaa attccataaa
    19201 agcggtaata gatgaatatg attatatatt tttagattgt ccaccctcac tggggatgct
    19261 tacaataaat tgtttaactg ctgtagatag tgttttaatt ccaatacaat gtgaatatta
    19321 tgctttagaa ggtgttagtc aattaatgga gactataaaa ttagtaaaat caagattaaa
    19381 tgctgatata gaaattcaag gtgttgtttt aagtatgttt gatggaagag ctaatttatc
    19441 aatacaagta gttgaagaag ttaagaaata ctttaaaggt agtgtatata caactttaat
    19501 tccaagaaat gtaagacttg cagaagctcc aagtcatgga aaacctgtaa tttattatga
    19561 taaaagatgt agagggtctg tagcttattt ggaactagca gaagaattta tagatttaga
    19621 agaggaggaa tggtagtatg gagaataagt ctaaaagaag taatagatta ggtagaggtc
    19681 ttagtgcatt gataccagaa attaaaggag aaacttcaga aaaagaaata gtcaacatag
    19741 atatagacaa aatatatcca aacgaagttc aacctagaaa gcaatttgat gaagaaaaga
    19801 taaaagtatt gtcggattct ataaaaaatt atggtgtttt acagccaata gtggttaaaa
    19861 tggatgaaaa taataaatat atgataatag ctggagaaag aagatttaga gcatcaaaac
    19921 ttgcaaataa aaatcaaata cctgctataa ttaaagatat agatatgaaa gatataatgg
    19981 agatagcctt aattgaaaac ttacagaggg aagatttaaa ttcaatagaa gaggctctag
    20041 catataagag tttaatagag cattacaatg taactcagga agaaatatca gaagctgttg
    20101 ggaagagtag acctcatata actaatacac taagactttt aaatcttgga caagatgtta
    20161 tagaaatgat agacagtggt agaattacag caggccatgg taaggcttta ttgagaattg
    20221 cagataaaga tttgcaactt caaatagcga aaaaaataga agaagaagaa ctttctgtaa
    20281 gagaagtcga aaatatagct aaaaaaatat ctgaaaataa acaagaagaa cctaaaaaat
    20341 ctaaacctaa ggatgtattt attttagatg ttgaagataa acttagaaat atatttggaa
    20401 caaaggtgaa tatttctaaa ggaaagaaaa aaggtaaaat agagatagaa tactataatg
    20461 atgatgattt aaatagtatt gtatcaatgc ttcttgaata gaacaaaaat ataaaacaga
    20521 gaggaagatt gttatgattt atttagataa tgctgcaact acatatccaa aaccagaaag
    20581 agtgtataat gctgttttag attgtatgaa aaattattgt gcaaatccag gtagagcagg
    20641 ccataaactt gctatgagag ctgctaggga gatatatgat acaagagaga atatagctaa
    20701 gttgttcaat gtaagtaatc ctatgaatat agtatttaca tctaatgcaa cagattcttt
    20761 aaatttagcg ataaaaggtg tattacaaga aggagaccat gtaataacaa cgtctatgga
    20821 gcataattca gtaataagac ctataaaagc attggaaaaa aggggtattg aaaatactgt
    20881 agtaaaatgc gattatgagg gatttttaga ttatgaagat ttagaaaaat caataaagaa
    20941 taatactaaa ttgattgtaa caacacatgc gtctaatgta tgtggaactt taatagatat
    21001 aaaaaaagtt ggagaaatag ctaaaaaaca taatatacta tttttagtag atgcatcaca
    21061 aacagcaggt gtttatgata tagacgtaaa tgaatgtaat atagatatgt tggcaatgcc
    21121 aggtcataaa tgcttatttg gacctcaagg aacaggtata ttatatgtaa gagaagggtt
    21181 gaacttaaat atattaaaag aaggtggaac aggtagtaaa tcagaagaaa tagttcaacc
    21241 agagttattc ccagataaat atgaatcagg aactcataat actccaggta tagcaggtct
    21301 taaccaaggt atactattta tatttgaaag aggaatcaac aatataagac aacatgaaga
    21361 agaattatgt caatatatga tagacaaatt agaggaagta ccagatataa agatatatgg
    21421 accaaaggac agtaaaaaaa gagcatcagt aatagctcta aatataggag atatggattc
    21481 tggagaggtt actttcttat tagatagtga ttataatata gcgactagat caggaataca
    21541 ttgttcacca ctagcacata ccactttagg tactttaaaa caaggtgcag taaggttcag
    21601 tataggatat ttcaacacaa aagatgagat agataaagca gtggaagctt tgaaaaaaat
    21661 ttcaaaaaat aaatagcatg agatttaata atatagaaat aaaaaaaggg gatatttcaa
    21721 aataaaaatt tgaatcatct ccttttttgt acaaaaaaga aaaggggttc ctaagaacct
    21781 ctaatctatt tgataatagt ttcaagaaat tactttaagt aatctccttc ttcaagaact
    21841 atatgaccac tttcgtaatt agaaccatca acagtaattt ttatttgttc aacattcata
    21901 ttttttatgt aagtacgagc aactgaatca agcattaaag tttctgcatc actacctaag
    21961 tttgactttg taaagtcaga gctaacatct aatataccag tttttacacc atcaatgtct
    22021 tttgtagtaa atgaatttaa ttttgcagat tctggaacaa ctttaagttt ttgaagttct
    22081 tcaaataaag tgttctcatc aattttattt aaatcaactt tattttcaat taaatctgtc
    22141 ttatctacat caaaagagta aatagtagct gattgagctt tagtttcttc ttttggagta
    22201 ggtttagctt tgttattagg aacctctttt tcagtattgt tttcttcttt tggttgctca
    22261 acatttgtat tgctcttagt ttctttttta gatgaatcac ttccattttg agcattagta
    22321 caaccaactg cgaatataga tatagataat atacttaaca ctaacattat tttcttattt
    22381 ttcataaaaa tttctcctct cttaaaaact attcttatat ataattatag tcgttatgag
    22441 caaatatatg aatacaaaat agtaacaaat ttaaaaaatg atttatttaa attaataaaa
    22501 ctcaaaataa actaaatttt agtcattttt atgggaaatc atttaatgta cagtgaaaat
    22561 attgtaaaat aaaagtagtg tttttaaaat aaatataagt tataattata ttataaaaaa
    22621 atgtcttcaa aaggtgggaa tagtatggaa attttgagct taggagaaaa gattaaaaag
    22681 ttaagaaaag aaaaaaacat gacattgaaa gaattagctg gagataggat aacagcagca
    22741 caaataagcc atatagagag agataaatct catactagtt atgaactgtt ggaatatcta
    22801 ggcgataagc tagatgtaag tgtagaatac ttattagaga ccaaggaaat gcaatcaaaa
    22861 aagataactg ataatttaat actacagagc gaaatttata taaaagaaaa tgaattaaac
    22921 aaggctgagg accaaataaa agaagtttta gatatatgta agagatacga tttaatggag
    22981 aactatggca gatgtaattt tttaatggct aatattaatt tgaaaaaatc taattataat
    23041 gatgctatag aaaactttga aaaggcattg tacttcttta taaaaaatag tgatagtgaa
    23101 aatatactta agtgttattt aaacataggg aaaatatata tgcaggaaga cttttataaa
    23161 ggtgctataa gccactttaa ttttgcagag gaaattttgt cagagagtaa atttgaggat
    23221 gttaacatac acaaggaact atatagtaag atggcatatt gttacataaa actagataat
    23281 ccaaatatgt cattgaagta catagaaaag ataaatgaaa tagataatag aaatgataga
    23341 aaagaagatg tcgatatttt ggtgttaaaa gcaaataata tgcttgagat tggaaaatat
    23401 gaagaatcaa gagattactt taaaaaagca ctaaaaatac ttgagagtga ggataataaa
    23461 actggattag caaatgtgta tttaacaatt tgtgatgttt acagaaaaat aggtgaaaca
    23521 gatagagtac ttgaatattc acaaaaggta tatgacataa agaaaaatga tgaagatgaa
    23581 tacatgatgt caggattatt taagataata gaagcatata tagataaaga agattatgat
    23641 ttagctagaa aatattgtaa gatagcctta gcttcatcaa taaaaaataa aaataaattc
    23701 aatgaatata aagcattgag attctatgct aatatgtata aaaatcagaa tgaagtaact
    23761 cttgcaattg aatatctgat taaatgcata aaaatagtat cagatttagg caacaataga
    23821 atattggcca atttatatat agacttaggt caattatatt caaatatatc aaaagaaaaa
    23881 gagttagaat attatcaaaa aggtgtattt atgtacaaaa atttagaaat aatgtagggt
    23941 aaataaacct ataagggtat agaaacaatc tataacaagc aagataatat ctatgataaa
    24001 atataaattg tatgaatatt tttaaggaga tgaatcagac aatgagtata aaaatagatg
    24061 cgagagggct tgcttgtcct aaaccagtta taaatacaaa aagagaattg gataacctag
    24121 aagaaggtgt tgtagttaca atagttgata atgaaacagc taaggagaat atattaaaac
    24181 ttgctaaatc tttaagttgt gaagcaaata ttgttgacga aaaagagaat tttatatcta
    24241 tagaaattag aaaaggtaaa aatatactag ataaacaaaa tgttgtagat aataaagaaa
    24301 gtgaattaga caatacatgt atatttatat cttcagataa gatgggactt ggaaatgacg
    24361 aattaggaaa agtgttaata aaaggattta tatatacatt aacagaatct aaaccttatc
    24421 ctaagtatgt acttttagtt aatggaggag taaaattaag tgctgaaaat gaagaaacca
    24481 tagaaaatct aaaaatactt gagaatatgg gtgtagaagt tttatcttgt ggtacttgtt
    24541 tagattatta taatttaaaa gacaaattac aagtaggtac agttacaaat atgtatacta
    24601 tagttgagac attaaagaat gcatcaaata ccatttctat ataatgtatg tatgtttaat
    24661 aatataaaac taacaataaa gattaactca aatagatagt cagaaaggat taagtaatga
    24721 atcaaatgta tatagtgtca tttaattcga cacatcatgc gataagatca gaaaagttat
    24781 ttggtgaaaa tagtctaaaa gtaatggctc ttccaacacc aagagaaata acagcaagtt
    24841 gtggaatatc aattaaattt tcttttgaag atatggaaaa gataaaaact attttggttg
    24901 aaaataatgt agatataaaa ggaatttatt gtataagtaa attagaaaat ggttcaaaag
    24961 aagtagagaa gttagattag gaggctaatt atttatgcca atggatttaa aaataggtga
    25021 tattgttgaa ttaaaaaagc aacatgcatg tgggtgtaaa gagtttgaaa tcataagaac
    25081 gggaatggat ataaagataa aatgtactaa atgttcaagg ctaataatgc ttgatagaga
    25141 gaccttagaa aagagagtaa aaaagctaat aaaaaagtga taaaaattta acacttaaaa
    25201 aatgtcaaat accgtatatt aatatccaaa attttggtat aataagaatt gaagaaaaca
    25261 ttttcatggg ggtgctttaa ttatggcagt taaatatgca aaaagaatgc aaggattaca
    25321 aggatcagaa atacgtgagc ttttaaaact tactcaacaa ccacaaataa tatcttttgc
    25381 tggtggaatg ccggctccag agttatttcc agttgaggaa atgaaaaaag tatcagttgc
    25441 agtgcttgaa gaaaatggaa gatcagctat gcaatatact acaacagaag gatatgaacc
    25501 attaagagaa aaaatagcag ctagaatgaa tgataaaaac aaaactaatg taaacaaaga
    25561 tgatatatta gttacaagtg gttctcaaca aggtctagat tttgcaggaa aagtatttat
    25621 tgatgagggt gatgtaattt tatgtgaaag tccatcttac ataggagcta taaacgcatt
    25681 taaatcttat caacctaaat tcatagatgt tccaacagat tctgatggaa tgataatgga
    25741 agaattagaa aagatactag aaacaactga tagaataaaa atgatttatg taatacctga
    25801 cttccaaaat ccaacaggaa gaacttggcc acttgaaaga cgtaaaaaat tcatggaaat
    25861 agtaaataaa tttgaaatac cagtaataga agataatcca tatggagatt taagatttga
    25921 aggagagact ttaccatctt taaaatcaat ggatacaaaa ggattagtaa tattcttagg
    25981 aactttctct aaaatattct gcccaggata cagattagga tggacttgtg cttctccaga
    26041 aattttatct aagtttaact ttgcaaaaca aggtgcagac ttacaagcat caactatatc
    26101 tcaaatggaa gtaagcaaat ttatggatat gtatgaccta gatgcacatg ttgacaaaat
    26161 aaaagcagtt tatgttaaac gtagagatgt aatgcttaag actatggaag aagagttccc
    26221 agaaggatta gtatttactc atccagaagg tggattattt acatgggtag agcttccaag
    26281 taatttaaat gctaaagaat taatgccaaa atgtttagat aagaatgttg cttatgttgc
    26341 tggaggagga ttcttcccta atggtggtag agaaaatact ttcagactta actattcaaa
    26401 tatgccagaa gaaaaaataa tagaaggtat aaaaaatata gcagctgtat taaaagaagc
    26461 tatgggtgta gaagcttaat taaaaataat aacttatata taatacaagg gatggttgtt
    26521 gccatccctt gtttgtttga aaagtatata attcacctta tactaaactt tgtccaaaat
    26581 ttaaattgac agaaataaga ataaatgata aaatgtgtag gtatgagatt ttagtctcat
    26641 ttctctgctc taagagtgta gagccgttaa gtccaaaggg aggtgaatta tagtgagaaa
    26701 ttatgaatta gtttatgtgg taaagccaaa ctctgatgaa gaagtaagag aggctatact
    26761 aaacaaagtt aaggaagttg ttgcaactga cggagaaata gttaaagttg atacttgggg
    26821 aactaaaaaa ttagcttatc caatagctaa gtttacagaa ggtttctatg tattagttaa
    26881 cttcaaatca gcagtagacg ttcctaaaga gatagacaga aacttaaaga taaatgaaaa
    26941 cgtaataaga cacatgatag ttgttgctta ataactggtt ttattataaa aaataggtgg
    27001 tgtttttatg aatcaagttg tattagtggg aaggttgact agagaccctg aattaagata
    27061 catcccaggt acaggaacac cagtggcatc ctttacaata gccattgata gagactatgt
    27121 aaaaaaagat ggatctaaag aaactgattt tatacctgtt gaagtaatgg gtaaatctgc
    27181 tgaattttgt gctaactaca taactaaagg tagattagta gcacttcaag gatctataag
    27241 agtagacaat tatcagacac aatcaggtga aaagagaact tttacaaaag taagtactag
    27301 atcagtacaa gctttggata gtaagaataa atcggaaaat tcatatagag agagtgctcc
    27361 agcttttgag ccaagctttg aacctcaagg tttagaccca caaggtttcc aagctataga
    27421 tgatgacgat ataccatttt aaaaaggagg gaaaaaagtc atgatgaata aaaaaagacg
    27481 taagaaaaaa agagtttgtc aattttgtgc tgacaaaaac gctaaaatag attacaaaag
    27541 tactcaaaga ttacaaaagt atataactga aagaggaaag atattaccaa gaagaatatc
    27601 tggaacttgt gctaagcatc aaagagaatt aacagttgct ataaaaagag cgagaaatat
    27661 agcactttta ccatatacat tagactaaaa aaatgagcct tgtgctcatt ttttattata
    27721 tgtatttata tatctaaatg aggcgagcta taaagctcgt ttttttgttt atatacttaa
    27781 atatattatt atttatgata taatattata tattaaggag ggttgtaaac gtgaaaatag
    27841 ataaaggttc tgagataacg agaaacataa agataataga atggatgaaa acagaaatat
    27901 taatgagtgt aagtgatttg tttaatttat tgtttaaagg ggtaaaacca ttagatgaag
    27961 caattcaaga tactttagca aatataataa tgataacata tctcttagca aagagattag
    28021 gaattagttt tagagatgtg gattataaag tcaaggaaaa aataaaaatt ggtatagatg
    28081 aggaccatag cgttgaaaga tggtatggag atttatctaa tttaaaaaaa catatggata
    28141 atagggagtg atattaaatt gaataataaa attagactat ctaaggcaat gtctataata
    28201 gttttagcta taataatcgc tttagtaata gcctatattc caatgctagg aatatttagt
    28261 atgttagcag ctgttccata tgtaattata ggtgctatta ctgatagaaa gtattctctt
    28321 atatctattt tggttacatt ttgtgtactg attctatttg cagacatttc ttatgcatta
    28381 aatatatgta taatgtattc tttaccaggt atagtaatag gaaagatgct aaagagaagc
    28441 tttgaacaag aggacagtaa taaattcgaa ccgatatatg gtggaaccat aatatttgta
    28501 ctttcaatgc ttgtatattt ttttgtatta aagacatttt taaatgtgaa tctattagat
    28561 gaagtttcta agatgatatc agaaattgta aatattcaaa aaaatagttt ttctgcaact
    28621 gaacttaaag tgtttgataa aatgaagcca gatgagattg taagttattt tacaaatatg
    28681 ctaccaatga tgttattttt acaagggttg ttatcagcgt ttgtcacata ttatttgagt
    28741 gtatttttta taaaaaggat tacaaagatg aatataagat ttcctaagtt tgctgatttt
    28801 tatttaccgg gaaatgctat actcacaact ttcttacttt atttattagt cttatttatt
    28861 caaattattg gttcaaaatt atatacagaa ttaataatga ctaatttaca gctagttttt
    28921 aatttaatgt ttgtaattca aggtatagca gtttgcattt atttcttaaa gaaatggata
    28981 agacagggac caaataaaat tatgtttttt tcagggataa ttttatgttt atttggattt
    29041 atgggaatat cttttgttgg tatggtagat agtataattg actttagaaa ggtaagaagc
    29101 tataaatcca cttaggagga tagaaatgag taataaacaa acctttaaat taaatatgcc
    29161 agaaataaat ttatatataa ttgttattgg tatttctagt ataattttgc tttattacaa
    29221 cttgtatgta gggtgtttat ttttctgtat ttttgtatat atggtttttc acaattggag
    29281 aactactaat attagacgtc atgaatggac agagtatatt caaaatttat ctttagatat
    29341 agatgaaaca actaaaaaag ccataataaa tcttccaata ccattatgta ttttagaatt
    29401 tgatggaaat atatcctggt ataatggaaa gttttatgat atgattggtc aaaaagattt
    29461 acttgataaa aatatagagg atatagttaa aaatcttaat ttaagaaaag tattaaatga
    29521 aaataaagag atgtacacag aaataaacta taaagaaaaa gaatatacaa taatttataa
    29581 tgttataaaa aatgatcaag aaaaaaatcc taaatatttg atgatattat actggataga
    29641 caagactgaa tatttaaaag taaaacaaaa ttatgatgat gaaaaaaatg caatgatgct
    29701 aatacaagta gatggatatg atgaagtatt aaaaagtgca gcagaagata agagagcttt
    29761 aattaatgtt gaagtagaga aaatactttc tgctttagaa ttaaattcaa atggtgcatt
    29821 gagaagaact tcaaaagaca aattcttctt ggtaatgcat aaaaaagagt taaaaaaatt
    29881 ggaagctgag aagttttcta ttttagatac aataagacat attgattatg gaaataatct
    29941 tcctgttact ataagtatag gaattggtat agatggagat acattaaatg agaacctaaa
    30001 acttgctacg ggtgctctcg atttagcact tggtagaggt ggtgaccagg ctgttgtaaa
    30061 aacaaaagat aaatttgtgt tttatggagg aaaatctaaa gctgtagaaa agaaaaccaa
    30121 agtaaaatcc agattaatag gtcatgcttt aagagaggtt atacaacaaa gtgaccaagt
    30181 ttatataatg ggacataaat atcctgatat ggatgctatg ggagcagcag ttggtgttta
    30241 tgatatatgt aaatcctgta acaaaactgc aaacatagtt ttacaatctg taaatgaatc
    30301 tatagaaata tttataaata aaataaatga aaataattac tataaaaagt tgtttattgg
    30361 aaaagaggaa gctattgaca actgcactaa aaatacatta gttgtagtag ttgatactca
    30421 tagaccaaat tatactgaat gtgaagaact gttaaagcta tcagagaagg tagtagttat
    30481 agaccatcat agaagaggtg tagagtttat aaatgatgca gtgcttttat tccatgagat
    30541 atatgtatcc tcaacgtgtg aaatggttac agagttagtt cagtatatgg atgaagatgt
    30601 aacaataaat aaacttacag ctgaagggct tttagcaggt ataagtctag atactaaaaa
    30661 ctttgcattc aaaacaggtg ttagaacttt tgaagctgct tcttacctta gaaaagttgg
    30721 agcagatact atagaggtaa agaaattctt caattctgat gtaaaagatt tcataataaa
    30781 agctgaaata attcaaagta ctaaaataat taataataga atatgtttag catattcaag
    30841 tactgagata gatagtataa atgttataat agctcaaact gctgatgagt tattaaatat
    30901 caaagaagta gaagcttcct ttgtattagg tgaaaaagat gatactatat ttataagtgc
    30961 gaggtcttta ggacaaataa atgtacatgt acttatggaa aaattaggag gcgggggaca
    31021 catagatata gctggagcac agttaaaaaa tgtttctcta aaagaagctt ataagatggt
    31081 aaataaaata atagaggagt atttggagga ggaagaatag atgaaagtta tattattaaa
    31141 agacgtaaaa ggaactggta aaaagggaga aatgaaagaa gtaagtgatg gatatgcaag
    31201 aaatttctta tttcctaaaa agatggctgt gcaagcagat agtgtagcaa taaaagaatt
    31261 aaatgaaaag aataaatcta aagaaataaa agcacaaaaa gagtatgaag aagcagtttt
    31321 attaggtaag caaatggaag aaattaatat agaaatatat tcaaaagcag gagaaggtgg
    31381 aagattattt ggttcaataa cttctaaaga aattgctgag caattaaaga aacaaaaaga
    31441 catagatgta gataaaagaa aaatattatt agatgaacca ataagatctt taggttcaac
    31501 tttcgtagaa ataaaaatac atcaaaaagt aactactaaa ataagagtgg atgtaaaaga
    31561 aaaacagtaa taactagagg tgattaagat ggaagatatg acgagaattc ctcctcatag
    31621 tgtagaatca gaacaatcaa tattgggttc tatacttcta gataaagatg ctataataac
    31681 ggttacagaa actataaagc ctgatgattt ctataaagag gctcacaaaa taatatatga
    31741 atgtatgata actttaagta ataaaggtga gcctatagac ttgataacat taacagaaga
    31801 actaagaaaa caagggcatc ttaatgatat tggaggaatc agttatatta caagtctttc
    31861 tactatagta ccgacgactt caaatgtaaa atactatgct gatatagtaa aagaaaagtc
    31921 tgtacttaga aagcttataa aagcttctaa tgaaataata aatctaggat atagtggagc
    31981 aactaagatt gaagatgttt tagaacaagc tgaaaagagt atatttgata tatcacagga
    32041 gaaaactagt gatgatttta aatctataaa cttagttttg atggatgcct atgatatgat
    32101 agaaaaatta tacactaaca agagtgatgt cactggtata actactggtt ttaaagattt
    32161 aaacaaaaaa ataaatggtc ttcaaaggac tgatttaata ttaattgcag ctagaccagc
    32221 aatgggtaaa actgcctttt ctttaaactt ggttcaaaat gcagccttaa aaggtgatgc
    32281 ctctgtagct gtatttagtt tagagatgtc taaggaacaa ttagtacaac gtatgttatc
    32341 ttcacaatct agtgtagaat taaaaaaaat aaagactgga acacttaatg ataatgattg
    32401 gcctagaatt atagatgcta tggcagtatt atcagatgcc aaaatacaca tagatgatac
    32461 tcctggtata aaaatatcag aactaaggtc aaagtgtaga aagttaaaaa tagaaaaagg
    32521 actagattta gtgcttattg attatcttca acttatggaa ggtgaaggta ataatgaaag
    32581 cagacaacaa gagatatcta agatatcaag gtcactaaaa atattagcaa aagaattaaa
    32641 ctgtcctgta gtagcactat ctcagttatc acgtgcacca gaacaaagag cagaccatag
    32701 acctatgtta tctgatttaa gagagtctgg agcaatagaa caggatgcag atatagttat
    32761 gttcttatac agggacgaat attatcatgc tgattctgag agtaaaaata taggagaagt
    32821 aataatagca aaaaatagac acggtgaaac tggttctgtg gaacttgttt ggcttggtga
    32881 agttcaaaga tttggggata agttgagaga cctataatct aacgaatatc cacagaacgt
    32941 cctttctttc tacaggaaaa atgtggatac atgtcactaa atatgtccaa acattgaaat
    33001 ttagctaaaa aactatccac actatccaca gatggcctgt tgataagtgt gaataagtag
    33061 ataaagcaag cttttggctt gctttatctg tgtataaata ttaattatta ggaggaataa
    33121 tatggaaaaa ataagcttaa aatatatata tcccaatata attaaagtac ttgatgaaat
    33181 aaacttattt agagtaattg acaataattt aagagaatct attgttgttt atgctaataa
    33241 tgtggataat cagtatcata taaacatgac taatacaaac tttggaaata taattaacat
    33301 atgtaaatta gaaaaacttt tagatgtgga taaatttatg gaaaaagtta taaaatatga
    33361 aaaagaaata atagagaaag aagagttttc aaaaatagaa gaatatatgt taaatattgg
    33421 agaatactaa aaaagttgaa aaaaatacga atgtttgata gaataagtaa gtaaagtgtt
    33481 cgagagtata taaaatatag aggtgaatta catgaaaaca gttgcaattg ttggttctca
    33541 atggggagat gaaggtaaag gtaaagttat agattatctt gctactcaag cagatgtagt
    33601 agtaagaggg caaggtggaa ataatgcagg acatacatta gttgtagaag gtaaaaaata
    33661 cgcattacat ctaattccat ctggagtttt aaatccaaat acagtgaata taatcggaaa
    33721 tggaatagtg tttgacccta aaggattttt agaagaattg gaaatgttta aaacggataa
    33781 tataagtaca gaaaatataa aaataagtga tagagcacat gtcatattcc catatcataa
    33841 agaactagat gcattgtcag aagaagccag aggcgattta aagataggta caacaaaaaa
    33901 aggtattgga ccttgttata tggataaaac agagagatct ggtattagaa tatgtgactt
    33961 aatggataaa gataaatttg ctataaaatt aaaagctcaa atagatgcta aaaatgaaat
    34021 agttaaaaat atatatggaa aagaagaatt atttgatttt gaaactatat ataacgaata
    34081 tctaggatat gcagagcaaa taagaaaata tgtggcagat acgtctgtta ttgtatatga
    34141 tgcagtaaga gcaggtaaaa aagtattgtt tgaaggagca caagggactt tattagattt
    34201 agatttagga acttatccat ttgttacatc ttctcatcca acatctggtg gatttgcaat
    34261 tggtgcaggt atagggccaa atatgataaa agacgttgtt ggtatagtaa aagcatatac
    34321 tacaagagtt ggagaaggac cgtttgttac agagcaaata aatgaaactg gagataagat
    34381 aagagaacaa ggacatgaat ttggggtaac tactggtaga ccaagaagat gtgggtggtt
    34441 tgatgcagtt atagttaagt atgcagctag agttaatgga ttgactagta tttcatttat
    34501 gttacttgat gtattaactg gatttgataa gataaaagtt tgtacgtctt ataaaatggg
    34561 cgataagata ataacggatt tcccagcttc attagatgat ttagcaaaat gtgaacctgt
    34621 atatgaagaa ttagatggat ggaatgaaga tataactcaa atagataact ttgatgacct
    34681 tccagaaaat gcaaagaaat atgtagcaaa aatagaagaa ctagttggag taagtgtaga
    34741 tatggtttct gttggtccaa atagagctca aactataata agaagaaata tatttgctta
    34801 atattttatc aattaaataa tttaaaatag ttgaagaaga gtatctcaag ataatgttta
    34861 tagttattag agatgctctt ttatatttga cttacattga ttttttatta agttaaagct
    34921 acttatacat attctaactg aaattaaagt tgaatttatg tagatacata taggtttttt
    34981 aaaaagaatt ttgttgaaaa taaatggcta aagatgattt taattatatt ctaaaaaaag
    35041 taaaaaaaac tattgactaa aaattatata gacgctataa tataacttgt gagtgaaaga
    35101 aacgacgaaa aaagtcgaaa aaatgtggtc tattagctca gtcggtagag cacctgactt
    35161 ttaatcaggg tgtcccgcgt tcgagtcgcg gatagatcac caaaatatgg ctcgttggtc
    35221 aagaggttaa gacaccgccc tttcacggcg gtaacagggg ttcaattccc ctacgagtca
    35281 ccagtatggg actatagctc agctgggaga gcacctgcct tacaagcagg gggtcacagg
    35341 ttcgagccct gttagtccca ccaaattttg aattagagat aaatactaga ttatatgctg
    35401 gtgtggctca acggtagagc agctgacttg taatcagcag gttgtaggtt cgattcctat
    35461 caccagctcc acaaagtaca taaattcaaa ttaatataat tttctatgtt acaggatact
    35521 tttaagtatc tttttttaat ttcattttta ttgtattaaa aatgaaatta aacatacaac
    35581 aaaattttga attacttgca atgtgatagt tttaaatata aaatttagaa tcattctagc
    35641 ataatattat gtaatatagc aaatttaatt tatagtatca ataaatatag actactaaga
    35701 atgtaaatga tttgttagat taaggtacat tagatatgta aatagaaaaa taaaaagtat
    35761 gttttaattt atgtgatata agttttaaac agagaagcaa gtttgctctt acctctctat
    35821 ttaaaaataa aaaaatgttt tttaagggct attttattta aatctttccc atctcaaatc
    35881 atctccagta aattctaaaa tcctaaactt atctaatatt ctagagagag ttctttgagt
    35941 gtaagtattt cctatttgag atggtgttaa gttggttgaa ataatgattt ttttaccagc
    36001 aacaagtctt gtatttacaa tattgaagat ttctccacta gtaaaagaat tattaagttc
    36061 agttccaagg tcatctatta ttaataaatc acaatcaaac aagtttttat aattatcttc
    36121 acttatttga ttatttgtgt ctcttctaaa tttataatct tcaagaatat ctaaaattct
    36181 aaaagaagtt tgatagatta caacattgcc tttatctaac aattcctttg caatacaatt
    36241 gcacatatag gtctttccta gtccagtaga accataaaac agtagatttt cactattatc
    36301 cttcttaaaa tcatgaacaa aattttcgca gatacttaaa ttatttagca tattttctct
    36361 aggagatatt tcaccatcag agcctttctt aggagaaaat atattcaagt taaaattcga
    36421 aaaattttcc tgacttaaaa tcctatctaa attagacatt ttataagctt catttacaat
    36481 ttcttgtttg aggcaagaac atttttctcc attaggtaaa aatcctttat ctttacaaga
    36541 aatacattga aactttattt ccaagtaatc aagaggtatg ttactttcag ctaaaagtct
    36601 ttctttcttt acttttaaag attctatatt ctctttagtc ttctttacaa tttcatcttt
    36661 agacttagga tttaaaagta ctatcttagc taaactgaga cctattttag aaatttcatc
    36721 atctatagat tttatctcag ggattctatt atatacctca ttttttctat gttctaataa
    36781 gagttcatta tcatctcttc tcttagcata tttagcaagt atttttctta ttttatcctc
    36841 attcatttaa tcacctactt aaacttttct ttttgacttt ttttaataat ctcatcgagc
    36901 tcatcagagg tatactgagt gaatgtttcg ttgaaattat gaaacttagt ttttttgtaa
    36961 gtattgttgt tttgagtagt attaatctgc ttcttattaa tattctcctt aactatacgt
    37021 tcttcctctt tttgctttaa atcatttaaa ttttttatat ttttttcatt ccaattttta
    37081 attataccat ttatataaga tatacttggg tttgaaatat tcttagactt agaacatgca
    37141 tttattatca aatctatatc catattaaat ttatcaaacc aggtgtccat aagttctttt
    37201 tctgattttg atggttgtct tgaaaatccc aattcattga aaatcgtttt atataaaata
    37261 tatcgttcac ttcttaccaa aaagctttct tcaacatctt ttggcgtata taaatttgaa
    37321 tcataccaat ttctaataat gccttcaatg tatttaactg gctttgatgt accagtttta
    37381 tctttgacgt attcatatgc gcaaagtatc atatcggggc tcatattgta tttattcatt
    37441 atatccataa tactcatctt ttcacttggg tcgagatatc gcccaactat tttatttata
    37501 gaattaaaca tttttctaat acttggattt tcagaagtag atacaacttt atctgtatta
    37561 gattttatag atgaattatt gcttaatata ttctcaatat agaaattttt taaatctaaa
    37621 aattctattg aataattaaa attatcatat tcttcattgt catgtatttt tataatcttc
    37681 ttttcttccc agaatttcca agcagataac acatcagaca atggtatatt taggtttttg
    37741 gctattgaat tattgtcgaa cttaggatta gaagtaatat cacatgcttg tctatatcct
    37801 aaaagataaa cttttacgta taacccgtcc gccattggca taaatatatc tataaagata
    37861 ttgggaatag tgatttcacc taaatctatt tcattgcttt ctttaaaaaa cattttatca
    37921 cctcagatta aattatatca ttttttatat tatatttgtc tgtaaacaaa taaaaggtca
    37981 caagattagc atgtaaatat tttctaaaag tattcatttt gaaaaggagg agatattttg
    38041 gttataaaat tcaaccaaaa agtaagaaga acattatcag tagcgattct atttatggta
    38101 ttaataatag gttgctttat agaaaaggat gacagtttag aaacatacaa agaaaaaact
    38161 aattcaaaaa taaatcagta taatatggat gttatatttg atgatgagac taagaggtta
    38221 atgtgcaatc agaatgttga ctacataaat aatacaaaat caaacataga taaaatatat
    38281 ttccatatat atccgaatgc tttttccaag aaagattttg ctccttttga aaaaagtgaa
    38341 atgacaaggg cttatcctaa tggatttaac gaaggatata tagatataaa gaatatatta
    38401 aataataata gtaaaatgga gtataaaatt aaaggagaca aaaatgacat attagaaata
    38461 gaattaggga aagagttaaa accaaatgaa agaatgtcat tagatataaa atataacgta
    38521 aaaataccaa attcaattgg aagatttgga tatggagaaa atactataaa tgttactaat
    38581 tggtttccta tagcttgtgt aaatgatgaa aggggatgga atttaaagag ttatgaaact
    38641 attggcgacc cattttatag tgagactagc gattttcatg tgaaactatt gattccaaat
    38701 aaatataaaa ttgctcatac aggaaaactt attgatgata aacatgataa taagaaaatg
    38761 ctatatgaaa tagaagctga aaacgttaga gattttgcat ttatactaag tagtaaattt
    38821 gatgtaaaca aagtagattc aaagggagta gctatcaata catataattt aaataaaaag
    38881 ctttctaaaa aagcaactga agtggcaaaa gattcaataa atatatttag tgaattattt
    38941 ggaaagtatc cttacaaaga attttctgtt gtagcaagtg acttttttat aggggggatg
    39001 gaatacccta tgcttgttat gatagaccaa agtttatata atgaaaaaaa tgaattttta
    39061 ctagaatatg ttattgccca tgagacagca catcaatggt ggtattctgc tgttggaaat
    39121 gatgaaataa gtgaaccctg gttagatgaa gcacttactg aatattctac aatagtttat
    39181 tttgaacaaa agtatggtaa agatatggca aacaagctta taaaaactat ggagatacag
    39241 acaaaaagtt atttgagtga aaatatattt aagccagcaa atgagtataa aaattctact
    39301 gaatacagct tgaatgttta tacaaaaggc gcaatagcat ttaatgaaat aagaaaagaa
    39361 gttggagata aagtattctt tgagacttta aatgagtact ataataaata taagcataaa
    39421 aatgctaatg gaagagcttt tgtggaacta tggaatagta aaggtgtaga tataaataag
    39481 attataagtg aatgtaagta aatagtgaat gtaagtaaat tttgtgcaga gtattaaatt
    39541 ttatttaata ctctgttttt ttggtataat ataatttata aggcagaaaa agaggtgaag
    39601 gaatgctaaa gtattgttca gtagggagtg gtagtagtgg taattgccac tatatagggt
    39661 ataaaaatac taatatacta gtagatgcag gattaagtgg caagagaata actacaggtc
    39721 ttaaagacat tggtgtagat gcagataaat taaaaggaat atttataaca catgagcatg
    39781 ttgaccatat aaaaggagca ggtatattat caagaaagtt tgatatacca gtatttgcta
    39841 atataaaaac atggtgttct atgaaggaca aattaggtga catcaaagat aaaaatatga
    39901 aagtatttga aaatgataaa acctactcat taggtgatat catagtaaga cctttttcaa
    39961 tagaacatga tgcgtctgac ccagttggat ataattttta tacagagagt gatgaaaaaa
    40021 tgtctatagc tactgatata ggatgtataa ctcaaaatat aaaaaaacat ctatataaat
    40081 ctaaattagt agtattggaa tcaaattatg accctaatat gcttatgatg ggttcatata
    40141 catatgcttt aaagaaaaga gtaatgtcaa gtactggtca tttatccaat gaagatgcag
    40201 ctaatttctg cgttgaactt attaatgaag gtacagaatc tatattatta gcacatttaa
    40261 gcaaagaaaa taattttcct gagttagcgt atgagacttc aaaaggagta ttagcatcaa
    40321 atgatatagt agttggacaa gatgtaaaac tagatgtact atcacgaaat gatgtttcta
    40381 atgtttatga gatgaagtag aggtgaataa ctttgctaaa gagaaatttt gaaaatgttg
    40441 aggataataa aattttatac tctataaagc agggtatgat tttagcgata cctgctatta
    40501 tgactggttc aactgctctt gtaattttga atcttccaat aaaggcttat caagaatatt
    40561 tatcaggctt gtttaatgga gaggttacaa atattcttaa ctttataaat gattctactt
    40621 taggaattat ttcattaata atattattaa caataagcta tagttatgga aaaatatatg
    40681 ggagcaagta tacagtactt gttcctatag ttgcaatgtg ctctttttta gtattttcac
    40741 atggtaatga gcttaattct tatatagaaa tatttaaaac aaagtggtta tttacatcta
    40801 taattgtttc tatgacatct tctgtattgt ttgtaaaact tacagaatct tttgttacaa
    40861 gtgtaaagtt tcatacagaa ggggctgatg cagactttaa tatggtagtt tcggctatag
    40921 ttcctttcat aatagtagta tttttgtttt ctatttttag agtggtaatg ataagtctaa
    40981 ttggcagttc aaactttcaa gatatatttt ataatttatt ttcaaatgtt tttaataaaa
    41041 tgggaacaaa ccttatgagt gctctgttgt ttattttttt aatgcacttt atgtggtttt
    41101 ttgggattca tggtagtaat gtattagata ccgtcgcaaa aaatttattt gaaaatagta
    41161 tggcaattaa cataaattta gttaataata atcaattacc aacagaaatt tttactaaga
    41221 ccttttttga tacaatggtt ttattgggag gatgtggctc tttactatgt ttagtaattg
    41281 caatttttct tagtgaaaaa cgtataaatg taagaaagtt agctaaaatt gcttcaatac
    41341 cagctctttt taatatcaat gagatgctag ttttcgggat accaattgtt tttaactata
    41401 ttatgtttgt accttttgta attactccta taatcttaac cgttacaagt tatgtggcta
    41461 tgtctacagg tattgtacca tgtacagttt cagctgtaga atggacctca cctatatttt
    41521 taagtggata tatgtctaca ggttcaataa gaggtagttt gttgcaagta tttaatttat
    41581 gtgttggtgt tatgatttat attccgttta taaaaatgtc gcaaaataga tatacatata
    41641 tgtttaaaaa taatatagaa aatttaacaa acttattaaa aaggtatgaa ttaacaggtg
    41701 aacagcctaa tcttctaaac cataatggta aaataggttc aatttcaaaa atgttggcat
    41761 cagacttgaa gtttgctatg aaaagaggag aaatagagct tttttatcaa ccacaagtca
    41821 attataatgg aaatgttgtg ggagcagagg gattacttag atggaaacat tcaataggag
    41881 ggttcattta tccaccacta gttataattt tggctaaaga agaagggttc ttagatgagc
    41941 ttggagagta tattattaat aaagcatgta ttgatttaaa gaagagtgaa aagctattga
    42001 gaaatcctgt taagttctct gtaaatatat ctccagacca acttgatgac cctaagttac
    42061 cagagcaaat aaaaaatatc attagtagta atgatataaa tcctaatatg ttaggtatag
    42121 aaataacaga gcaaattgca ttatcaggtt cacaaataat tattgaacgt atcaatgcga
    42181 tacataactt gggaataaag ttaattatgg atgattttgg gatggggcat agctctctta
    42241 tatatcttca aaataataac tttgatatag ttaaattgga tggttcatta gttaaagaga
    42301 tacttacaaa taaaagaagt agtgaaatta taatgactat tgtaaatctt tcaaaaaatt
    42361 tagattttga tatagttgtt gagtatgttg aaaatataga ccaaagggat aagttatatg
    42421 gtttaggatg tgaaatatat caaggatatt attatagcaa agcaatacct tttgaagagt
    42481 ttgtagaata tgcaaatcaa gaaagagaga tataatttta taagagcttg taaattctag
    42541 taatgcatag atttgtaata aaattaagtt taggagaaat atatgaatat aagtatagta
    42601 gtagtaggta aaattaaaga aaaatattta aaattaggta ttgatgagtt taagaaaagg
    42661 ctttctaagt attgtaagtt agaaattata gagttggatg atgaaaaagc tccagagaat
    42721 ttgagtgtta aggaaatgga aattataaaa gacaaagaag gaaagaaaat tttagggaag
    42781 ataaagcata atagttatgt tatagccttg gctattgatg gtaagggttt atcctctgaa
    42841 gagcttgcta agactatgag tgatttagca gttagaggta atagtagtct ctgttttata
    42901 ataggaggtt ctttagggct ttctgatgag gttttaggaa gggctgatta taggttatcc
    42961 ttttctaaga tgacttttcc tcatcagatg atgagattga tattgttaga acagatttat
    43021 agggcttata ggataaataa tggagaaccg tatcataaat aaatgatacg gtttttgtta
    43081 atttgacact taataatatt aaaataacaa aaaagtattg aaagtatatt gcatttaagg
    43141 tagaattaaa taaaatatta taacattata atgtttatat ggttgttaat aaaaatttgg
    43201 aggaaaatat atgataaaaa taatgttggc ctgttcagca ggaatgtcta caagtttatt
    43261 agttactaaa atggaaagtg cagctaaaga aaatggtata gagtcgcaaa tttgggcaat
    43321 accagaatca actatacaaa atgaaataga aaaatgtgat gtattattac taggaccaca
    43381 agttagatat gtattaccta aggctcaaga aattgctaag ccatataata taccagtaga
    43441 agttataaat atgatgcact atggaacagt taatggagaa gctgttttaa atagagcgat
    43501 agaacttaac aacactaagt aatataaatt ataatgtatt tttaatatat aaattgggag
    43561 aatcaaagga tggatgaaaa gactatagaa atatctttta atataatagg ttatgcaggt
    43621 gaaggtaaag gattagcttt tgaagcaata aaagaggcta agaatggaaa tatagaaaaa
    43681 gcaaaagaat tattaaaaga gtccagggaa gttataaata aagcacatag atatcaaaca
    43741 gaattaatac aaaacgaagc tagtggaaac aggactgaaa taagtgtcat acttatacat
    43801 gcacaggacc acttaatgaa tggcatgaat tttcaacagt tagctgagga aatcgttgat
    43861 ttacatttga aattacaaga taaataaaaa ttaaagataa taaattaaga gagttcttaa
    43921 aagatacatt atgtgtagca cactaattgt tagattattg attcaatatt tagtgagtaa
    43981 tacattagga ttattttgag gactcttttt atatttagtt taaagttaat aaatatttat
    44041 tattaataat ataagattat atgttataat attataacat ataatttaag aggtgaatca
    44101 tgttaagaaa tcaaccatta tatatacaag ttgtagatat attaaagcaa aaaatattaa
    44161 ataacgaata taaaattgat gaaaatattc caacagaaag ggagttagag caagagtttg
    44221 gagtcagtaa aattactatt agaaaagcta ttgagatatt agaatcagaa gggtatgtag
    44281 agaaaaaaag tggaaaaggg acaaaagtaa taagtaatag tctttttaat aagctatcaa
    44341 aagcagagtc tttttcaagt atacttttat ctaaaggtca taagttatct aaaaagacta
    44401 tttcaattga aaaaataata aatgatacaa attcacaagt atttagtata tttggagaaa
    44461 aatgtagcaa gattactaga atgtattact tagatagtaa gccatatata ttttttactc
    44521 attatattcc ttttgatgaa aatctaactg ttgaaagttt taataatgaa agttctttat
    44581 atatgtactt atataggaga aatataaata taactagatt tgaagatgaa ttttttatag
    44641 aagaatcaga actagaggta tcaaaggctc taaatctagc taaagacata ttattaggaa
    44701 gaaaaagaaa gacctatgat gagaatgata atatagttga gctatcattt gcaagatata
    44761 atacagaagt acaaaactat gtaattaaat acgaaatata ataagaatcc tgagtttatt
    44821 gaaatgataa tatgctcagg atttttgttt agttgtaaaa ataaaaaata ttataacatt
    44881 ataatgttta caactaaaca ttataatgtt ataatatttt taataaacaa gtgatattat
    44941 caattacata aaaattagtt tatgttatga aatcgttatt gcattataaa taaaggataa
    45001 tatcaataat acagggggat aactatggaa aaattcgcaa gttttttaga gcgcaagatt
    45061 atgcctgtag cagcgaagat aagtaatcag agacatatga gagctgtaag agcaggtata
    45121 atagcaacat taccactaac aatagtgggg tcatttttca caatattatt aaacatacct
    45181 attgatgctt atatggattt tatagctcca tttaaagcaa tattagatat accatttaga
    45241 tatactgtag gatttttatc tctatatgct acttttggga tagcatcatc acttgctaaa
    45301 agctacgatt tagatacaac aggtgttggt atgcttgcag ttatggcatt tttagtttct
    45361 acaatagttc caactcaagt agttgaacca gtaggtggag ttatagaagc tggtagatgg
    45421 atggatatgg caaaacttaa ttcgccatca ttatttggag ctatcgtaac atcattaatt
    45481 acagttgaaa tatatcgttt tatgaaggaa aagaatatta caattaagat gccagcagga
    45541 gttccagtag aggtttcaaa ttcatttact gcattattac cgacagcagt aattttaatg
    45601 ttattctggg taataagaca tattttagga tttgatataa gtgttgcact aagcgctatg
    45661 ttatcacctt taaaaggatt cttatcagga aatagtttag gtggtgggtt actgacagta
    45721 ttcctaataa ctttattctg ggtacttgga atacatggtc cagctataat ggggccagta
    45781 ataagaccta tatgggacat gtctatagca gaaaatataa ctgcattcac agaaggagta
    45841 tcagctcatg caatgccaaa catatttaca gaacaatttt tacagtggta tgtatggata
    45901 gggggagcag gggctactct tgcattagtt gtaatgttta tgttttctaa gtcacaatat
    45961 ctaaaaagtt taggtagatt aggattttta ccagggttat tcaacataaa tgaacctata
    46021 atatttggag caccaatagt tatgaatcca atactaggga taccatttat aattggacct
    46081 ataataatga caattctatc atatgttctt actattacag gggttatacc tatgatggta
    46141 gcaagattac catttgcaat gccagcacca atagcagcag taatgagtac taactggagt
    46201 atattagcag gtatcttagt attaatcaac tttgtgatat catttgctgt ttattatcca
    46261 ttcttcaaag tttttgaaaa acaacaatta caaagagaac aagaagaaat gagtgaaaat
    46321 ttataaaaat acatagatat agaggttttg tactatataa aatatagaaa aaacttataa
    46381 gttattaagg gtgaaggtag gaggaatgac ctttgccttt aattttatat attttaaata
    46441 atgtaatttg aggaggagaa agatggaaag aagattagga atatcagtat atccagagca
    46501 tagtaacatg gaagatgata aggcatatat aaaaatggct tcaaaatacg gatttaaaag
    46561 aatatttatg tgcatgcttt caattacaag acctaaggaa gaagttaaaa aacattttaa
    46621 agagattata acctatgcaa gagaattagg atttgaagta atccttgata tagctccaaa
    46681 catatttgat atattacaaa tatcttatga tgatttaaca ttttttagtg agttaaatgc
    46741 tagtggaata aggcttgact atggatatga tggaagtaaa gaagctatga tttctttcaa
    46801 tccatatgat ttaaaagtgg aactaaatat gagtaatgat gtagcatact tggataatat
    46861 aattacttac aagccaaatg taaataatat atatggttgt cacaattttt atcctcaaag
    46921 gggtacaggc ttgccatatg atttttttgt aagatgttca aatagattta aaaagtatgg
    46981 aataagaact gctgcttttg ttaattcaaa atatgcaaca atcggacctt ggaatataaa
    47041 tgatggactg tgtacgcttg aaatgcatag aaacttagat attgctacag caactaagca
    47101 cttattcgca acaggattga ttgacgatgt aattgtaggg aatgcatatg caagtgaaga
    47161 tgaattaaaa agaatgtctg aagtaaataa atatcaagtt gtatttaatg tagtaactga
    47221 ggaaaaaact tctgaagtag aaaagacaat aatggcaaat gagcaacact ttagaagagg
    47281 ggatataacc aaccaagtag caaggtctac agaagtaaga aaaaaatatg ctaaagaaaa
    47341 tttccctaag catgataata cgaccatctt taaaagagga gatgtagtga ttggtaatga
    47401 tgactttgga aagtataaag gggaattaca gttgatactt caagattgtg cagatgaaag
    47461 aaaaaataag gtaggtagaa taaaggaaga cgaaataatg cttttagact ttattgaacc
    47521 ttggagtaaa tttaaactta ttttagaggg agagtaaagt gaagatagac atattaataa
    47581 aaggtggaaa aacagtagat aaaagtataa ttgatatagc tatattagac gataaaataa
    47641 tagaagtaaa aaatcagata gatgattcta agtatcaggc aaatcaaatt ataaacctag
    47701 atggagaaaa atttattagt gctggttgga tagatataca tacgcattgc tacgaaagac
    47761 tagaccttta taaggattat ccagatgagg ttggaataaa aagcggtgtt actactgtag
    47821 tagatgcagg aacaacagga gctttagata taggtaaatt ttatgaagat actaaaaaat
    47881 ataaaacaaa tgtatatgct ttaataaata ttgctaagca gggaataaca tctcaagatg
    47941 aactttcaag tatgatgaat attgatgaat atgaacttaa aagagcagtt aaaaaataca
    48001 aagattttgt tgttggaatc aaagctagaa tgagtaaaag tgttgtaatt tcaaatgatg
    48061 ttgaaccttt aaaagtagct aaaagaataa aaaatgaact taatctacct atgatggttc
    48121 actttgggag ttctcctcca acgatagagg atatatttga ctatatggaa aaaggagata
    48181 tattaaccca tatatataat ggaaagccaa atggaatact tagaggcaat gaggttaaaa
    48241 aagaaatcat agaagctaga gaaagaggta taatccttga tgtaggtcat ggaaccgaaa
    48301 gttttagtat ggatattgca atgaaatcta aggatgcagg aatttttcca gacactataa
    48361 gtacagatat atatattaaa aatagaataa atggtccagt gtataatcta tctacaacaa
    48421 tggaaaagtt tatatatatg ggctattcac ttgaagatat aatagataaa gttactaaaa
    48481 atgcagcaga tgcaataagt ttaaaaaata aaggtttaat aaaagaagga tatgatgctg
    48541 atttaactat atttgatgtg gttaatgaag aaaaagaact tcaagattct ttgaataagt
    48601 cagtaatcac aagtacgtct ataaagccaa gagcagtagt agtcaatgga gaatatttaa
    48661 atataggtaa gtctatagga gagaatgagt agaagggaga aactattgta tgagtagtat
    48721 atttgaaaaa tactctgtaa gacgtgttat aaatgcttca ggcaaaatga ctatactagg
    48781 agtatcaaca gtagataagg aagttattga agcaataggt gaagggtgta aaaatttctt
    48841 tataatagaa gaacttataa ataaaacagg tgaatatata gcaaatttat tagggtgtga
    48901 aagtgcacta atagtttcta gtgcatctgc tgggattgca cagagtgtgg gagcaatgat
    48961 agcaaaagat gatatgagtc tagtttacaa tgtaaataat cctaagaaac aagttaaaag
    49021 ggaaataata attccaaagg ggcataatgt tgattatgga gttccagtag aagtaatgat
    49081 aagacttgga ggaggagaag ttgttgaggc cggctatgct aatatgtgta acaaggaaca
    49141 tataataaga gagataaatg aaaatacagc agcaattatg tatattaaat ctcatcattg
    49201 tgttcaaaag agtatgctaa gtgtattaga tgcagtacaa gttgctaaag aatataattt
    49261 acctttgata gtagatgcag cagcagaaga agatttaaat gtatattatg aattgggagc
    49321 agacctagtg atatacagtg gtgctaaggc tatagagggt cctagctcag ggttagtaat
    49381 aggtaagaaa aaatacatag ataatgtaaa gcttcaatca aagggtatag gaagagctat
    49441 gaaaatagga aaagaaaata ttgtaggtct aacatgtgca attgagagat atataagtaa
    49501 tgataaagta actttaaaag aaatggagga caagctaaat ccatttatag acaagataaa
    49561 tactataaag ggagtgagtg ctagtataac tagagattca gcaggtagag aaatacttcg
    49621 tggagaaatt gactttaatg aagatataat aaataagtca acacaacaaa ttataaatga
    49681 acttagaagt ggagagatag ctatatacac tagagactat aaggctaatg aaggcaagat
    49741 tgagattgat ataagaagtg taactggaag tgatttagat actatttata ataggataaa
    49801 aaatatagta gaaagttaga gtgtagagta agtgacatta taatgattta ataaattaaa
    49861 ttttaccaat ggaggggata aatatgaatt gtataccaca atattataaa aatagagttt
    49921 gtttaaatgt tcttgcagga agcataaaaa atgctaaaga agtatatgat gcttgtgatg
    49981 ggtatgtatt gataggagta ctatcaaaga attacagtac tgtagaagca gctattgaag
    50041 atatgaaaaa atactcaaag gaaatagata atgcaatatc tgtggggctt ggagctggag
    50101 acccaaatca gtcaaatatg gtatctcaga tttctaaaga acttcagcca caacatataa
    50161 atcaagtgtt tacaggtgtg gctacgtcta gagcattgct tggtcaaaat gatagtataa
    50221 taaatggatt agtttctcca actggtaaac caggatttgt aaaaatatcc acaggtccat
    50281 taagttcaaa agaaaaagat ggtatagttc ctatagaaac agcaatagct atgatgaaag
    50341 atatgggagg aagctcaatt aagttttttc caatgggagg gcttaaaaca aaggatgaat
    50401 atatagaagt tgcaaaagct tgtgcaaagc ataattttta tttagaacca acagggggta
    50461 tagacttaga taacttcaaa gagatagttc aaattgcact tgatgctggt gtagaaaaag
    50521 ttatacctca tgtttataca tcaataattg ataaagaaac aggtgaaacg aaagtggaag
    50581 atataggagt actttataaa ataatgaaag aattattggg atagatttat gaatatatta
    50641 gcttttggtg aaataatgat gagactttca gtacctgact acaagttctt aacacaaacc
    50701 aatgaactaa attatataat tacaggcaca ggattaaata tattatctgg acttaaaaac
    50761 tttggataca atacctatat gttgacaaag ttgcctaata ataatgtagg taaagcatca
    50821 agtgcaaata taagaaagct tggagtaaaa gatgacttta tcacatatgg agggaatcat
    50881 ataggagtgt attttcttga aaatgggtat ggagaaagac cttctgaagt gacttatttg
    50941 aataggttaa atagttcatt ttgtgagagt aaattacaag attatgatat tgataaatgt
    51001 ttagaagaaa tggatatagt ccttatttgt ggtattgcta tgcaacttac agattcaatt
    51061 aaagagattg ctttagaact agctagaaaa tcttatgaaa aggggataaa agtattcttt
    51121 gattttaatt ttagacaatc attaaatcca gaaaggactt atgatgattt agtaaatgat
    51181 tataagaaaa tactaccata ttgtcatggt ctatttgcta gttatagaga tataaataag
    51241 atacttaata tagatagtct aaatataaaa tctagttcag aattgattaa aaaagagtat
    51301 gatataggta ttgtagctgg aactatgaga agatttgatg atatgaataa tagatttata
    51361 aagggatata tatcaaatga tgatggttat tttgaaagta gggagtataa gttagaaata
    51421 tatgacaggg ttggtgccgg agatggatat gtagcaggaa ttatatattc atatttggaa
    51481 aatatgaaga gtgaagatat agttgagttt ggggttgcta gttcggtgtt agctcataca
    51541 acttatgggg ataattcttt agtaagcaca ggtcatgtac ttagacttat gaacaatgaa
    51601 aaaatagata tattgagata atacatttgt atataataaa atttttatct tttttagaca
    51661 cacttttgac ttgtttatat ttgttaaaca gagttatttt taatgattat aaatattaaa
    51721 ttaaaagtta aataataaaa tagtagaaaa tagcattaac tatagaaaaa atggttaatg
    51781 ctatttttat cgttaatatt gattaatttt atattataga aatgaacttt tctaatgctg
    51841 gatttgtatt ttttttattc catacaacta caacttcaat tctattttct acttctttta
    51901 taggtactat tgcaatatca cgttctatca ttgtatctgt aacacaaaat gaaggtaaaa
    51961 tggatattcc catgttagaa gataccaatg attcaatagt attaagatta tttgaacgtt
    52021 gtaccacatt aggtacaaac cctgcttcac gatatctatt ctgtatcatt gacttttgtc
    52081 catactcatc ccctgtttct tttacaaaaa taaatttatc atgttgtaat tcaattaaat
    52141 cgatagaatc ctcattgtaa agtggatgac caggaggtaa aagagctacc aaagaatatc
    52201 tttctacagt tttatattca aaatctttta atggttgatt ttctggatta ataacaaaag
    52261 cgaggtccat agaattattt tttactaagt ttaaaagttc ggttatttca ttagtacaaa
    52321 tatccataga tatattaggg tatgtattag aaaattcttt taaatattgt tgaagtttgt
    52381 ttttttcata tcctattaag gttccaatgc tcaaagttcc aaaaaaacca tgtgcaaatt
    52441 gttgtgtttt tgcaatagca tcattgatat tttttattat agtacgtgcc tcagataaaa
    52501 aaacttttcc agctggtgtt agttcaacat gacgtttact tcttgtaaat agagttacat
    52561 ttatttgttc ttctaaagct tttatttgct gtgtaacagc agtttgagaa atataaaatt
    52621 tttctgctgt tttggtaaaa cttaaatttt ctgctacaga tataaaatat tctaattgtt
    52681 tagtattcaa ataatcaccc ctatttaaac ttgattgttt gtaaacctta tttatctata
    52741 attaaagctt attatagtat aagaaaattt acttttaaag aatattagaa aatatttgta
    52801 taaaaatata tgttttaagt atatctttac attagatttt ataaatttat taataagaat
    52861 aacttatata ttccaatgaa aacgatattg gatttaagta agtttgtctc ataaaatgaa
    52921 agtatatcaa ttgataatac tttaaataag aagggagaaa aaatggaaga taaattttat
    52981 gcaaaaggca acggaaataa cggatatatt aaaaatcttg aagtttgttc ctttaataac
    53041 ttagatggaa cttgtggaat gtttcaaatg gctctgtaca aaagagatga aaaatactat
    53101 ttatatggat gctgttttgg aggaaataaa aaaaatggag taatgattag cgatattaca
    53161 gacccttata atccacaatt tataaaacat tttcaaatgt tagaccctaa agagtatcct
    53221 acaacaacaa ctcccaaaat tcaaatagca gatgatttaa tgatagtagc aatgagttgt
    53281 ggaagtggac caggagcact tgttgaccaa gctaaattag caaatattaa gtgtgaagca
    53341 ggaattagaa tatatagttt aaaagaagac cctttaaatc ctaagttttt aggatattgg
    53401 gattgtggct taaagcatgt aatgggtgtt catagattca tgtacaatgg tggaagatat
    53461 gtacatttat caagtgattg tgttggcttt gaaggtctga tttatagggt catagatata
    53521 ataaatccta ctaatccagt ggaaataggt aaatggtgga gaccagacca atatgcagat
    53581 ggatatccaa atagaacttt tgatgcagga gcacctcatt gcccagaatt tatggataaa
    53641 ggatggcttc atggacctcc atttgtaaga gacggaaaag catattgtgg ttatggagga
    53701 gctggtttag ttgtattaga tgttgaagat ttaacaagac caagatgctt aggtgaattg
    53761 ccatttatgc ctgcattttc tagtagactt gcaggtgcaa gaactcatac agcattacca
    53821 ttgccaggaa gagatttagt cgttgttcaa aatgagggag aaagattcca gttctttaaa
    53881 ccagataaca ttacagatgt tcaagctatg aataatatac atatggttga tgttagtgac
    53941 ccaacaaaac caacattaat tgctcaattt ccatatcctg aagttccaaa agatttccct
    54001 tatcctaact ttaatgttgc aggattagga aaaccagggc catttggtcc acataatctt
    54061 catgaaccaa tggataataa accatggtta gagcaaagag gagatagagt atattgctgt
    54121 tatttccatg cagggctaag ggtttatgat gtatcagacc catattatat caaagagcta
    54181 gcatatttta taccaccaaa tccaaataaa acaccagaag aatcttattt cccaggattc
    54241 ccaggaccac gcttggcagt aacagaagat cttatcgttg atgatagagg ctacatcatc
    54301 atagatgctt tagatgatgg attctatata ttaaaaatga aagaagatta aataaatact
    54361 aaagagggag agttgaaaat gaaaatacta ggtatttctt ttggaacaaa aaatggaaat
    54421 aatgacacaa tgtgtaaagt tgcacttaaa ggtgccaaag aagcaggggc tgaagtagaa
    54481 tttattcaaa tgtcatctat taacattaaa cattgtactg gatgttgtgc gtgtgttaaa
    54541 actttattat ctgggaaagg tagtatgtgt gtattaaaag atgattttga atggttgctt
    54601 gacaaaatga aagatgcaga tggtatagta gtttcagacc ctatttttga agaaggggca
    54661 tcaggattat tccatactat aatggaccgt tttggaccca gagcagatag aggaaataac
    54721 attataggaa caaaagttgc agaagctata ggaggaaaaa ttccagattc aagaatgctt
    54781 aatgataaag taatatcatt tatgggaata ggaggttctg actggggtac aagagttcaa
    54841 tgtgaacatg ctatgctagc tctcatacca atgtggaaag ttatagataa tgcttggttc
    54901 ccatgggcaa aggaattagt aatggatgat acaaggcttg ttcaagttca tcaaattggt
    54961 ttaaatatag ttgaagcagc aaaagatttt gaaaaagctt catatcaagg agaagaaggt
    55021 gtatgtccac attgccacaa tagattattc taccttgagc caggtactaa aaaggcaatt
    55081 tgtgctctat gtggtatagt tggagagtta gatactataa gtggaaaaac tatatttagt
    55141 tttccagaag agcaattagg tcatgctcat gatactttac caggtaagtt tatacatggt
    55201 gatgatatta agaaaatgga aggtcattat gcagaagttc gtaaaacaca agaatttaaa
    55261 gaaagaaaaa catcttataa ttttatagag acattaacta aagaaaaaca agctggagtt
    55321 tcattaggat aataattaaa atactaagac tcttttttaa gggatgttag atgttatagg
    55381 aggcttattg tatgaatgaa aagccaagtt ttaaacaaat tgaagttatt ttagcagtta
    55441 tttttttggt acttacatat tacaaggtta gattaactcc agcatttatt gttatattta
    55501 taccactata tttaagttat tttgcagtat ctatgattgt aaatcaaata gcaattaagc
    55561 tattggagat aaggcaagct agggaagcta ctaattacaa tataggaaat ggatagaaag
    55621 aaaaaagaaa tattaacact gtctatgggg attgctttac tacctccttt atgggcagta
    55681 ttagcacctt atataggaat aaaaactgga gctgtagcac taatctgtgc aggattatat
    55741 gtaactaatg gtaataaaca agaagatgga ttaaaaataa tgtttggttt ttggtgtgga
    55801 gatttatggg cagtgttagc cattttaatt atgggatata tgaatttcaa tcaaaaccta
    55861 gaattatttt taacacttag tatattaggt ttttttgcag ttgtaattgc atccttattt
    55921 gaaaaaataa ttttcctacc atcatggctt tgtggttggg ctataggtct aactattatg
    55981 acaggtgaaa atattattaa tttacaagat atttgtattc aaattgctgt agctatggca
    56041 gtaggagtat ggtacgtggg tgcaggtgta gatttgttta caatatatat tttgagtaaa
    56101 gataggaaaa agggaaattg aaagcatgtg aatatacttt gattatagga ggatggctat
    56161 gggttttgaa tttaaaataa tgagaagttt aatatatgta ggacttgcca aggaagaata
    56221 tagacctaag ctaatggact ggttatatcg tcaccatatt ccagatagta ttagcacttt
    56281 tggaccatat tgtactaaat atgcctttta tcaagcatat cctacaccaa atgaaggtga
    56341 gcgttttggt gcacgtaaga tgcaactaac agaacattat tggcttgtag atgaacatat
    56401 gcctgagatg gcaaatagaa ttatgacaga atatatgcct atggatgttc tacgttggca
    56461 agggtgtata ccagatgtag aaaataaaag ggttcatgaa aatgcagaaa gtggagatgc
    56521 aggacgtgca gtaggtggag ataatggatg tccaccattt atatttgcct ttgttccaat
    56581 aaactgggaa gaagacttta gaggaaaagg acgtactgta caagatggac caaactatcg
    56641 ttggcaattt atgattaagt atccagatgg tatctctaaa gaagaaggag aaaaatggtt
    56701 ctatgatgag gtagtgccat actttacaaa ctgttgctat gttaatcgtt ttgtcagtag
    56761 taaaataatg attaattatg gagcaactgc ttttgaccgt gtgtcagaac tatggtttga
    56821 aggggaagaa gaatggtata aagctgtggt tgaagagaca aagtcgttta ttaaaaaacc
    56881 agaatgggca caagaagagg agttcccata tttaaaacca caattcaata tcgcatcagt
    56941 attcttaggt gatatagcaa ctatggatgc atactcacag tatcgtggat atataccaat
    57001 gagataaaaa ttatagtttg tgataagtag agaaggcatt tactcttact ggtagaagaa
    57061 gacttattgt atccttaaaa gtaggtgaat atatagtttt atatttaaat ttatataaaa
    57121 ctaagactaa taaatatgaa acaatagaat tagaataatt tgataatatt tgttaaaaga
    57181 gcatttagaa tgtgtttaaa gattaagata ttccttttat ggtagcagat agttgaaata
    57241 ataaaactgt tatcatgaaa ggaatatttt tatgtatagg aaatttttaa aaataaaagt
    57301 taaatatgtt taagaacatc ttaaagtgaa agaatcattg ttagaaatat gtagacaata
    57361 tagtatttat aacgtatcat attaacaagt ttacacttgg attatgaaat aaaaagagta
    57421 attctaaagg ttgtaaatac tatgctgact ttgatacttc aaagtttagg tgattttgta
    57481 attgttaata gaaatacgtt taatgagcat aaagaattta tctaataata ggagaatata
    57541 atgtatacaa ttggacaagt atctaaattt ttaggaatat ctagagatac actaaaattt
    57601 tatgaagaca aaggattagt aaatcctaag aaaaatgatg aaaatggata tagaatatat
    57661 aatcaggttg atatctatga tattgcaaca ataaactttt atagagaaat tgatatagaa
    57721 attaaaaaga ttcaagaaat aagaaaaagt aaaagtataa acaatttaga actcttacta
    57781 gaagagaagg aacaaatcat actaaaagag atagaatata aaaagcttct cttaaaaaag
    57841 gttcagttag tcaaagaaga ttgtgacaaa atcaaaacat attttggaaa atacacaata
    57901 aaagaaatga agcctttgga agtaaaaggt aaaataaaac atgttactaa ctatgaggaa
    57961 tatgaagcct taaaagagaa tacaaacaac ttaaaaaaag cagttacatt aactgattta
    58021 agaagagtaa taaattttaa tgaagaaggt ataatagaag aaaaatttat aattgtaagg
    58081 gaagtagatg actacaacaa aatcatagaa ggtgaagttc tatctcatcc caattgtatt
    58141 tatactatta ttgaagatgg cagatggtca actggaggag aaaatattga taataaagtg
    58201 gaagacagat taagagaaat agcaatagaa aatgaatgtg aattacttgg aatagtctat
    58261 gttaatatat tgcttactac ttatgaagaa gggattgagc gtatattttt agagatttat
    58321 gcacctataa aataatataa aagcattgac ctgggactta ctcataggtt taaactcatg
    58381 atataagcag ttataactta tatagcaatt aaatattaat ttgaaatgag gattacttat
    58441 gagaacagag aaatcattaa aaaggcctat agccagtttt attattctaa caaatataat
    58501 ttttttacca ctttttttat tggtagtagt gactataatg ttaaaacttc caacagtgat
    58561 atctgacatt gcgttgtgta tatcagcgtg gtcatccact tttgcattta tgatattatt
    58621 taaaaaaatt tatccaggta agaaattttt agtacatgta aaagatagat ttagaaataa
    58681 acttaaattt tcaacagtat ctataataat tagtatacaa gtcctaatag ctgtagtagt
    58741 tatatttata ttagcatcta aaaatcatgg aat
//
LOCUS       DAEPIN010000017        56363 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.017, whole genome shotgun sequence.
ACCESSION   DAEPIN010000017 DAEPIN010000000
VERSION     DAEPIN010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 56363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56363
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.017"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            162..551
                     /locus_tag="KR250_001244"
     CDS             162..551
                     /locus_tag="KR250_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBH0067204.1"
                     /translation="MKQQEVVNKLRRGELMLMEYLWRKNSILSKKEIIEAMKEKYKWR
                     KSTTEILLKRLVKMKTLKKKRIGFQFSYEVLVTKKEYLNAIKEDRKVTKYDSFFIRMF
                     TTMHKKEKMTEEQIKKYIENMEKIGKE"
     gene            645..1028
                     /locus_tag="KR250_001245"
     CDS             645..1028
                     /locus_tag="KR250_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBH0067205.1"
                     /translation="MGFKKLPDSELKVMKFIWGLDGNKVASGEVVEAMKQIYDWNKNT
                     TLTILSKLAKKNYLYSQKTSKCTYYTIAVREKDYLKVETKKFFSFFHNNSLKSFFTAL
                     NDEENLSDDKLDMLEEWVKNWEEDE"
     gene            1707..3137
                     /locus_tag="KR250_001246"
     CDS             1707..3137
                     /locus_tag="KR250_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBH0067206.1"
                     /translation="MILNQIFIEAGGIMRNKIIIGITFSLVLLMSGCASKQTAEINVL
                     ATTDLHGVIPYELTSYVKEERKKDKNLTLVDAGDFFDSGEVFGSSMDSYFSARYKDGD
                     NNTESYIETPISKDMKEVGYDVAVLGNHEFISNNKFYLDNMISDFEKQKINILSANTY
                     KKNGDSYTKPYIIKNINTPEGNVKLGILGLTIKEVGERKQWKDGKLVDSKSLELKDQE
                     GYNGELYMNDLVEDAKKWVGIMEKEKPDIIVAVAHTGEKPKKPKNPGNRIQDLAQQVD
                     GIDAIVAGHNHVQIEQHDYKNKSGENVIVTEPGKHGECISKINFKLEKNNDKWNIVDK
                     SSKLIKFEKNQESDKFGEFISSINKVSKDKKEIRLSEVKTPEWDKIYVFKSGTSADDI
                     YKTIGYKWRNISSPKGDDMVQIVVMNNNKPVSYVYGEAEVMPINIKCDESEYKDNVVT
                     IYPNKNDKFKIEKGKQEYSINLKHIQ"
     gene            complement(3285..3635)
                     /locus_tag="KR250_001247"
                     /pseudo
     CDS             complement(3285..3635)
                     /locus_tag="KR250_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904812.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            3630..4151
                     /locus_tag="KR250_001248"
     CDS             3630..4151
                     /locus_tag="KR250_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HBH0067207.1"
                     /translation="MFSIGGGYAIILLIQEKVVNQTGWISEKMFTDIITISQMTPGPL
                     AVNTSTFVGLQIGGFWGAVAATLGCVLCGVVISLFLYNFFQKHQNSTYIFEILNGLKS
                     ASLGLIISAAATIIILALFGTNKVYFNSIFEMLNWTALIIFFVTLLIVRKWKINPIVI
                     IIISGILGIFFYL"
     gene            complement(4280..5074)
                     /locus_tag="KR250_001249"
     CDS             complement(4280..5074)
                     /locus_tag="KR250_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBH0067208.1"
                     /translation="MIFCNSFITRKSSMNIVVTHTPIVTTLEMQKAYEPLAKHNVNIF
                     AFDFSGTGKSDGYEKDFSRNSIVKDLDSVITYIEKNYSSNIHLYGNTGIGGMFAQYYA
                     CSDNKLKSFAQFACVDYKNTTGVGYTYLTVKLLNFFFKFLPNFHITVKPPKYKGYNEV
                     YDNEFYKIFEKKNPHIWKNSTKIINTMLECFIAKDSKIKDGVNIPTLVFKTLHDRYFK
                     TQYFDSYYNSLKCKKKLVEINNVHNSYYIDSEKFCQEAYDWFVENQ"
     gene            complement(5298..8726)
                     /gene="uvrA"
                     /locus_tag="KR250_001250"
     CDS             complement(5298..8726)
                     /gene="uvrA"
                     /locus_tag="KR250_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBH0067209.1"
                     /translation="MKINNYKNQSIITNPKKFENKYQDLPKTPIELLKVVQSLVIHGD
                     QGKLYGISFNKQQSDEELLRTIPQMLKRIFEINSNPLTIPRNPKQRLVGMCRDYSLLL
                     VSLLRYRGFEARMRAGFANYFESELTYEDHWLVEYYDTLTKRWIRIDAQIDDIQKNYF
                     QINFDTHDVGKTDGFLTGSEAWIRCRQGHAHQDDFGYNKNWKGWHSVKGNLLHDFNNM
                     IGLELLPWDLWTELSSKKYNQLTRAEKNLLDEMAEILSSGNIKIEDLNLLIEKLPEDY
                     LKSIFSQLKILGISEIKELGNPLELEKKFKFTKSINKSIKNSLCHNKSSIYLKGGRQN
                     NLKDVEVTIPKNQITVITGVSGSGKSSLAFDTIYEEGKRRYFENLSNGAKLSEQLQKP
                     EFDLLQGLTPTIAIEQKKGSQNPRSTVGTLTSIWDYLRMLFVSIGKSYCPYCKIPLEK
                     KNNTKNYCPHCQTIFSKINTSTFNANSHTGACHDCNGLGFTYQVNPQLIVKDPTISIL
                     DGATYYFGKLKGKKPNGNWMVGELYAIAKDKNIDLDIPWNELPRDFIDAILYGTDDKI
                     YEFSFESKGRESKIRRPASGAINHIQRLFRESSSENNTLHQYMNKIPCNTCGGELLCI
                     EARFTTIKGYRFPELTKMTIEQLWNWLCELPNQLQKNELSLVNDILTELKIRVSYLLK
                     VGLSYISTDRTAPTLSGGELQRVRLSSQLGSELVGLTYILDEPSIGLHPRDHNLIIKM
                     IEELRDKGNTVIVVEHDKDTILSADYIIDVGPSAGTKGGFIIAEGTTQEIIKNPNSIT
                     GKYLSTYNKISSQNKIIPSKWLSLKGCHANNLKNIDVEIPLNCMCSITGVSGSGKSSL
                     VFHSLLPALEEKLKQKSIPDKNYTEFTGFDAIDDFILMDQTPIGKSSRSTPATYINIF
                     DEIRSLFAETPQAKQKLLDESYFSFNSKKGQCPNCQGLGKTKIILQYMADQWVTCSEC
                     QGKRYQKEILSIQYKGKTIADILDMEVAEAKTFFSDCSDIYRKLSLLDEVGLGYLKLG
                     QNTLGLSGGESQRIKLAKELGTKTKKRMLYILDEPTTGLHFKDIENLLITFRKLVNEQ
                     HSLLIIEHNTEVIRASDWIIDIGPDSGINGGEIVASGTPDEIKINPNSITGHFI"
     gene            9045..11279
                     /locus_tag="KR250_001251"
     CDS             9045..11279
                     /locus_tag="KR250_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBH0067210.1"
                     /translation="MKLSRYKKNSLVLSIILCSLIISILVITVFNTVKLHNISNKQSK
                     NYLNDVSTQIVMNVDSKIKFILSDLRIMADFIKQYEGDSRYEYLSKRKTSYKYYDVGI
                     IDLEGKAEFLSGKKFDLKNTVSYKKGIEGKEYSEVIESMGFVLYSVPIFNDNHEVSSI
                     LIGVSNKESMNEILNIDNFNGKGTIEIINSKGKPLFIGKNTELIKDLSHKYKSSGDEP
                     WAQKMLEDFKNDKSGNITITSSKGVQCLLTYHPITRGLNDWHFLLIVPEDAVLGELNK
                     LNSFTITMTFIITCIIGIITLILYIIRRKYVNQIENIAYLDSITNGINSTKFSMLVKP
                     LISESPDSTYMMIAMNIKDFKLINDCFGSEKGNMTLKHLYNILYKNIDENEEFVCRHD
                     ADLFYLLIKNRPVLEALELLYKIEADTNYFNKNRENPYFLRLSVGIYTIENHNEDLIT
                     IQDHANTARKSFNKTHQSDFSFYSDIERKRLISEKEISNLMEKALENKEFFMCLQPKI
                     DIKSGKISGAESLVRWKNSEKGIIYPSDFIPLFEKSGFICKLDLYILEETCKLISKWI
                     KEGKEVLRISVNVSRQHLNDKLFLEKYKHICNKYNVPTCLIDLELTESIFLENPEAID
                     IIEDIHSNGFKCSIDDFGFGYSSLGILKDFKVDIIKLDRSFFVSKNNIDRGKVVIKSI
                     IELSKRLGMKVTAEGIEELEQVEFLKNVGCDYIQGYVFSKPLLISDFENFAYENNKIK
                     NIHY"
     gene            11535..13565
                     /locus_tag="KR250_001252"
     CDS             11535..13565
                     /locus_tag="KR250_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBH0067211.1"
                     /translation="MISGRMLLIMNFLKGKNKTSYKDISKQLSIEERKVRYDINNLNI
                     VLRSLNKSVIQKMNKGVLIIPDDFQVVCINNNEYVFSLTERVSIMKIISMFKIDNLNL
                     EKLSKEFQVSRSSIKNDLNVLSSELERNQITITYDKSFKISGEEDIIYKQRLNILKSY
                     SYLFGKEKSDLSVFEKYLVNVIEKIFKGIYLIDIYNWSMNLLLQMGWTLNDESFTWYV
                     SNIFLFTWYIHSDEKHPLESASLSEPCFENKFDQEFETIIQKKLTKEQIFLLKSFVFF
                     TNKYASLNEDMDLISTEIIVQDLIYNMSEILEISFKEDMILYKGLLNHIAPLIERIKQ
                     NIQIYEELLHVIPDKYQYVLEATKVSIQKNRVLSQIKNENETILLAIHFLGSIHRNER
                     NNYKNILLVCGFGYGVIAMLKDSLVNDYQINIVESIPSYKLQDYKDWESIDFIITTSK
                     IVIDLPKTIIEINPFLQEEDYLKFEEKGIKRKNVLTNYMSIKKRLDFLEEDAQKRVLS
                     IICQELGYSDERILFRQLNLSDLIGIDTIRIIDGDMKWEDAVKFSSNILANCDFVSAQ
                     YAENIIDILKNVGFYAVKDNEFALLHGNDSSLVKVSSISLLICKEAVSFGDKKVKIIF
                     CLASRDKKEQIPAIINLTKMVYKTNFIAMLESAKTKEEAEVLIHKYEKEVTV"
     gene            13562..14014
                     /locus_tag="KR250_001253"
     CDS             13562..14014
                     /locus_tag="KR250_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBH0067212.1"
                     /translation="MNKLVNVKCSFKIHVNSKEEAIISLVDVVSKEGYLIDKNQFLKD
                     VLKREETLSTYIGHGIGLPHSQSVGVKNSCITIGKLDTPIEWTEEGEKVDLIFLISVT
                     KDNENNLHLKILSKLARLLMHESFRNQIRCSDEQTVYNLIKEKIEEED"
     gene            14016..15071
                     /locus_tag="KR250_001254"
     CDS             14016..15071
                     /locus_tag="KR250_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HBH0067213.1"
                     /translation="MRILKFLQKHLMTATSYMIPFVVAGGILFALSVTLSGQAAVPET
                     GWLAKLNQIGAAGLALFIPILGGYIAFSMADKPGLAPGMIGAYLAKEVNAGFIGGIIA
                     GFIAGFVVLQLKKIKLAPTMRTLGSIFIYPLVGTLITGGIIVFLIGEPIASFMTWMTN
                     WLNGMSGVSKIPLGGILGGMIASDMGGPINKVAATFAQTQVDTLPYLMGGVGVAICIP
                     PIGLGLATLLFPKKFSKEERDSGKASLLMGCVGITEGAIPFATSDPVRVIPCIMAGSI
                     VGNIMAFLLGCLNHAPWGGLIVLPVVDNRLGYIASVLTGAVVVAVLMKLVKKDVKEDE
                     EIEEDLDESIELIFEEL"
     gene            15138..15446
                     /locus_tag="KR250_001255"
     CDS             15138..15446
                     /locus_tag="KR250_001255"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HBH0067214.1"
                     /translation="MKIVAVTSCPSGVAHTYMSAESLELSAKKFGIEIKVETQGSSGI
                     DNELSLKDIDEATCVILTNDVEIRNMERFKGKKVVRMSVSEIIKKSDALIKKIKDTFQ
                     "
     gene            15550..16815
                     /locus_tag="KR250_001256"
     CDS             15550..16815
                     /locus_tag="KR250_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II D-tagatose-bisphosphate aldolase,
                     non-catalytic subunit"
                     /protein_id="HBH0067215.1"
                     /translation="MKKLPIKTVVQRLLNLQEEGKSATLLGIGPMSPNLLQASFELAK
                     DDDFPLMFIASRNQVDADELGGGYVNGWNQETFTKDIKKVADKVGFDGLYYLCRDHGG
                     PWQRDKERNDHLPVDEAMELGKKSYLADIEAGFDLLMIDPTKDPFEIGKVIPLDVVLE
                     RTVELIEYCENERKRLNLPDIGYEVGTEETNGGLTSTETYETFITRLKVELDNRGLPM
                     PTFIVGQTGTLTRKTEQVGTFNFRNAYDLAQMAKKYGVGLKEHNGDYLDDVTLLEHIP
                     SQIIATNVAPQYGTEETRAYLKLAEVECKLEKEGLVEKTSNIRHVLLVNAIECGRWRK
                     WVVGEQKNLTTEEIFKDEVLSNEILDIAGHYTFNNDDVKKEIEVLYDNLSKNNIDGQR
                     FVVDHIKRPLRDYAECYNLKGVTTRILNK"
     gene            16903..17619
                     /locus_tag="KR250_001257"
     CDS             16903..17619
                     /locus_tag="KR250_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBH0067216.1"
                     /translation="MINLIFDVDDTLYNQLTPFYTAYNKVFSSIKDISIEDLYMSSRK
                     YSDEVFHMTENGEMPIKEMHIYRIMKAFEELGNSITEKDAQSFQDEYIYQQSQITLIP
                     EVERILNFSKERNINLGIITNGPSNHQRMKLKQLNIENWVDKSNIFISSEVGFSKPDT
                     NIFRVAENVMNLDRENTYYVGDSYRNDVLGAKKAGWKSIWLNHRGHEVEELFYKPDFV
                     ILEHKDLISLFIKICSYKNM"
     gene            complement(17814..18107)
                     /locus_tag="KR250_001258"
     CDS             complement(17814..18107)
                     /locus_tag="KR250_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein"
                     /protein_id="HBH0067217.1"
                     /translation="MRNMIENLIKNNTYIDDKVIMLSMLSSAKASANMYLNSALTSST
                     PELRAIYSASLTQMVEGHTALTELSINKGWVKPYDKPIQQLTCSYKESQNVID"
     gene            complement(18113..18328)
                     /locus_tag="KR250_001259"
     CDS             complement(18113..18328)
                     /locus_tag="KR250_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0067218.1"
                     /translation="MRILSETEKISLAAVIKMESDGLLMQRAINMLISDEDLKRQSES
                     SILATEGRIKAMQQFIVENEILISEEV"
     gene            18777..19175
                     /gene="tnpA"
                     /locus_tag="KR250_001260"
     CDS             18777..19175
                     /gene="tnpA"
                     /locus_tag="KR250_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011291581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HBH0067219.1"
                     /translation="MYKSNKNIVYDCKYHIVFCPKYRKPVLVGEVEKMLKEILPYKAD
                     ELGAEIIEMETDKEHVHLLISCDPQYGIHKVVKGLKGFSSRVLRENFPHLKSSMPSMW
                     TNSYFVGTVGSVSLEVVKQYIENQQVRTKK"
     gene            19172..20335
                     /locus_tag="KR250_001261"
     CDS             19172..20335
                     /locus_tag="KR250_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBH0067220.1"
                     /translation="MIKTHRVKLNLTRTQFELVREKQMESANCWNYIVNLSKEYYFEH
                     KQWIKKNDIQKLIKGKYNLHSQTIQAISDKFDANRKTISELRKKGNTKAKYPYKTKKF
                     YIIPFKASAINRNAKGNLKLSMSKGRYLELDFNVENIKTAEIVWRNGYYLYYTFDNEL
                     NGVVAKGTNTVGVDLGEIHSIASVTNEGVGLILSNREGRSIKQFRNKMYAYISKRLSK
                     CKKGSRQSKKLWRLKNKIRSKTDNQLMNLYHQTTRKFIDFCVEQKVCEIVLGDIKGVE
                     KDTKKKKRLNRVNRQKISQMEYGRIKDYIKYKAKEQGIEVKLVKENYTSQTCPKCSKK
                     HKPTGRTYSCTCGYETHRDIVGAWNILNKKHKYGLVDFRINHKQPINLKVSTV"
     gene            complement(20719..21231)
                     /locus_tag="KR250_001262"
     CDS             complement(20719..21231)
                     /locus_tag="KR250_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBH0067221.1"
                     /translation="MLEVIKNRRSIRTYIDKNIEEDKITEILKSAMQAPSSKNAQPWE
                     FIIVDDKELLKQLSKSQHRAKHIEFAPLCIVVLGNRDKFLKPGKWIQDLGACTQNLLL
                     EVTNQGLAACWAGVFPKNKVVNKVRQTLDLPLKLVPYALISIGYSEEKNEFIDRFDEN
                     KIHRNVYKNR"
     gene            21681..22157
                     /gene="purE"
                     /locus_tag="KR250_001263"
     CDS             21681..22157
                     /gene="purE"
                     /locus_tag="KR250_001263"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HBH0067222.1"
                     /translation="MKVAVVMGSKSDYPKLEEGIKLLEKYGIEVVARALSAHRTPEQL
                     SIFLKEIEDDTDVIIAAAGKAAHLPGVIASQTLIPVIGLPIKSSTMDGLDSLLSIVQM
                     PKGIPVATVTIDLGLNAALLALQIMTLKYPKLKEDLKSYREEMAQKVLEDDKNLRG"
     gene            22163..22861
                     /locus_tag="KR250_001264"
     CDS             22163..22861
                     /locus_tag="KR250_001264"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HBH0067223.1"
                     /translation="MLLYEGKAKQVYSTDNENEYVVYYKDDATAFNGEKKAEISSKGI
                     LNNKISTIIFEMLKENNINTHFIKSLSDREMLVKKVEILPLEVIVRNIAAGSICKRVG
                     LEEGVVFDEPIFEISYKNDAYGDPMLNDDYAVAMKLATREELKFLREETLKINELLKA
                     FFLKLNLKLVDFKIEFGKDSEGNIILADEVSPDTCRLWDVNTNEKLDKDRFRKDLGDL
                     VEGYTEVLSRMNNK"
     gene            22873..24240
                     /gene="purF"
                     /locus_tag="KR250_001265"
     CDS             22873..24240
                     /gene="purF"
                     /locus_tag="KR250_001265"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HBH0067224.1"
                     /translation="MCGVLGIYSNKDVTKELYYSLYSMQHRGQESCGLALLDDGEIKY
                     KKDMGLVGDVFKENELSKLKGNIGIGHVRYSTAGGSHVSNCQPLVGSCRKRQLAIAHN
                     GNLVNANYLKDMLEEDGYMFQTNSDTEVILYILARYYKGDIVESLKVTMDYIKGAYAL
                     VIMSQEELVAVRDPHGFRPLVLGKKGDEYIFASENCAIDILGGEVIRDVEPGEIIVVK
                     DGELKSYFYSENYKPVKKSCIFEHIYFARNDATIDNVNAYEFRIKCGERLAQNETVKA
                     DMVVPVPDSGWPGAIGYANASGLKISEGLVKNRYVGRTFIKPTQEEREIAVKIKLNPL
                     STIIKGKSIILVDDSIVRGTTSKQLVKSLREAGAKEIHLRITSPPVAYSCYYGIDTPN
                     RSKLIASSNNVEEMREYIGCDSLKFLDIEGMLDATEHKSTFCKACFDGEYPVKKIDKE
                     ELLSC"
     gene            24234..25310
                     /locus_tag="KR250_001266"
     CDS             24234..25310
                     /locus_tag="KR250_001266"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HBH0067225.1"
                     /translation="MLTYKESGVDIDEGNRAVDLIKGKIKGTYDGNVVGDLGNFSGLY
                     SLKDFVGMKEPVLLASTDGVGTKLKIAQMMDKHDTVGIDLVAMCVNDLICQGAKPLFF
                     LDYIALGKLVPEHIEKIVGGIADGCKMSGCALIGGETAEMPGMYGEDDYDLAGFSVGI
                     ADKEKIVSGNNVKSGDVLVGISSSGVHSNGFSFIRKIFLETYNYKMEQYVEELGMTVG
                     EALLTPTKIYVKLALDVLAKHDIKAIAHITGGGLIENITRVIPKGLGLDINKKSWEKP
                     PIFKMIEGFNAVDERELHKSFNMGIGLVLIVDKENADDVVNFINNRENDNAAYVDKKY
                     SELLEDKAYIIGEVVDSHEGVELC"
     gene            25304..25897
                     /locus_tag="KR250_001267"
     CDS             25304..25897
                     /locus_tag="KR250_001267"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HBH0067226.1"
                     /translation="MLNIGVLISGGGTNLQAVIDGTESGEIKGQVKVVISSKQGAYGL
                     ERAKNHNIKAICETDEDKIIEILKENKIDLVVLAGYLKIISPKLVNEFRNKMINIHPS
                     LIPSFCGAGFYGEKVHQGVIDYGAKVTGATVHFVDEGADTGPIIMQDVVKVNQDDDAK
                     TLAKRVLEVEHRILKESISLFCENKLKLQGRRVFINE"
     gene            25890..27422
                     /gene="purH"
                     /locus_tag="KR250_001268"
     CDS             25890..27422
                     /gene="purH"
                     /locus_tag="KR250_001268"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HBH0067227.1"
                     /translation="MSKRALISVTDKTGVVEFAKELNKLDYEIISTGNTFKTLKENGV
                     NVMQVEDVTNFPEILDGRVKTLNPYIHGGILYKRDKESHVETVNEHKIHSIDLVAVNL
                     YDFEGTLKAGKSHDEIIENIDIGGPSMIRSAAKNYKDVIVVVDIKDYDSIIEKLKTDT
                     MTLEDRKKLSYKAFSTTGRYDALISSYFAGEVGDTYPDILNLTFQKEQTLRYGENPHQ
                     NGFLYSQSNAKNPILNYEQLGGKELSFNNLNDLHGCLEVMREFKDSEEVVSVAIKHAN
                     SCGVGLGKDAFEAYTKCYEADKVSIFGGIVGITSTIDKATAEKLNEIFLEIVVAYDFE
                     PEALEILKQKKNLRILKLAKIENSLQPYEMKYLDGKLLIQDRNNILAEKSENVTKEKP
                     TDAQLKDMEFGMRVVKNMKSNAIAVVKNGQTLALGCGQTSRIWALKNALENNKDKDFT
                     GAVLASDAFFPFDDCVTLAHEYGISAVVQPGGSIKDKDSIEACDKYDMVMVFTGIRHF
                     KH"
     gene            27433..28683
                     /gene="purD"
                     /locus_tag="KR250_001269"
     CDS             27433..28683
                     /gene="purD"
                     /locus_tag="KR250_001269"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HBH0067228.1"
                     /translation="MKILVVGGGGREHAICWKLSKEKNVEKIYCAPGNAGIANVAECV
                     NIGDTNIEELLKFAKENEIGLTIVGPEVPLVMGIVDEFEKEGLRVFGPNKKCAQLEGS
                     KAFSKEFMIKHNIPTAKYKEYTNLEEAISEIDSFGYPVVIKADGLAAGKGVVIPENRE
                     DAIATLKEMMSDKKFGAAGDKIVIEEFLKGIETSILAFVDNDTIVPMASAKDHKKVNN
                     YEQGPNTGGMGTFSPSEIYTEELANKVKETVLEKTLEGFKKDGLNFKGILFVGLMITE
                     DGEKVLEYNVRFGDPETQSVLFRLETDLHEIMEAILDNKLKDIEINYSDDEAVCVMLT
                     SGGYPDSYEKGKIITGLENLDDDIVVFHSGTKMFDGNLVTNGGRVIGITAKSTRVKDA
                     AEKVYENIKKINFEGMHYRTDIGR"
     gene            28708..32514
                     /locus_tag="KR250_001270"
     CDS             28708..32514
                     /locus_tag="KR250_001270"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HBH0067229.1"
                     /translation="MLNTENKDSMVRRVLVEKREGFDLEAKALKKDLVESLHIDNIEN
                     LRILNRYDVEGISEEVYENAAKTIFSEPNLDVVYYEEIPKLNDERVFAIEFLPGQYDQ
                     RGDWAAQCVQIVNQGIRPAINTAKVYILSGKITDEEFSKIKDYCINPVDSREASLEKP
                     ETLKMETEIPTTVEVLDGFIDLDENGLRTFVSEKGLAMTLGDLQHVQKYFKDTEKRNP
                     TITEIKVLDTYWSDHCRHTTFMTEIENVKIEDGKFNDIVKEAYQMYLNSRDNVYVNRH
                     KDICLMDIATVAVKELKKNGKLNDLDESEEINACSINVDVEVDGKMEKYLVMFKNETH
                     NHPTEIEPFGGAATCLGGAIRDPLSGRSYVYQAMRVTGSADPRTTLEDTLPGKLMQRK
                     ITTEAAHGYSSYGNQIGLTTGQVAEVYDENFVAKRMEIGAVIAAAPKENVVRERPEAG
                     DVIVLLGGKTGRDGCGGATGSSKEHSEESILTCSAEVQKGDAPNERKIQRFFRNKEVA
                     QMIKRCNDFGAGGVCVAIGEIADSLDINLDLVPKKYDGLDGTELAISESQERMAVAIK
                     KENKDKFIQLAVEENLEATHVATVTDTGYLRMFWNGKAIVDINREFLDTNGVKQTTDV
                     HVTKVDEENTFFSSNEIVKDVKCASMKDKFTKVLSDLNVCSQKGLVEMFDNTIGGNTV
                     LMPFGGKYQATPTQGMVAKIPVLGGETNTSTIMTYGYNPKVGKWSPFHGALYAVVESV
                     CKLVAIGGNYSTTRLTFQEYFEKLGNNPEKWGKPFSALLGAFYAQSKFEIPAIGGKDS
                     MSGTFKDIEVPPTLVSFAVDTVDAKKVVSPEFKKADSKVVMLCVNKAENDVVDFEELK
                     RNLDKVRELIHGNKVLSTYALGFAGVGEAISKMAFGNKIGFKFSEEAEKAFTDNKLFE
                     ASYGNIVLELANDDLSMLEGYNYVVIGSTVKEASIFIKGEELALDELYKAHCSTLEPI
                     FPTKTEEVKSKIETISYISQGEAKKSSLSIATPRVFIPAFPGTNCEYDSARAFERAGA
                     NASIRVFKNLTYKDIEDSIDTIVNEIKSSQIIMLPGGFSAGDEPDGSGKFIATVFRNP
                     RVQEAINEFLTQKDGLMLGICNGFQVLIKLGLVPYGEIRVPSESAPTLTYNNIGRHQA
                     KIARTRISSNKSPWLAQTNVGDIHNIAISHGEGKFVASEDVMRELIANGQVATQYVDF
                     NNEATYDIEFNPNGSFYAVEGITSADGRVFGKMGHSERIGEEVYKNIIGEKDQKIFES
                     GVKYFR"
     gene            32720..33592
                     /gene="rfbD"
                     /locus_tag="KR250_001271"
     CDS             32720..33592
                     /gene="rfbD"
                     /locus_tag="KR250_001271"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HBH0067230.1"
                     /translation="MKILITGSNGQLGKELVNQLEAINQSINQPKYVILATTRSDLDI
                     SNQTNVDNFILHNKPDVVVNCAAYTKVDACEDNIEIAYKINALGVRNLAIASEKVNAK
                     LIHISTDYVFNGFSKYPYREDNKTEPNSVYGKSKLMGEKFVEQFSHKYFILRTAWLYG
                     NGNNFVKTMIKLSLENKEVNVVNDQFGTPTSTVDLAKIIIRIMETEHYGVYHATCEGE
                     CSWYDFAKKIFKLKNIDIKVNPIKSNEFKCKAPRPLYSVLDNFMLKLIGLNSFRKWEE
                     SIEEYLERYDINEK"
     gene            33701..34570
                     /gene="rfbA"
                     /locus_tag="KR250_001272"
     CDS             33701..34570
                     /gene="rfbA"
                     /locus_tag="KR250_001272"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HBH0067231.1"
                     /translation="MKGIILAGGSGTRLYPITKCISKQTLPIYDKPMIYYPMSVLMLA
                     GIQDILIISTPRDIKAFEELFGDGSSLGLNITYAIQEKANGIAEAFIIGEEFIGNDRV
                     SLVLGDNIFYGYGFSERLENAVKRKGATIFGYHVSNPNSFGVVEFDKDFNVLSIEEKP
                     TVPKSNYAIPGLYFYDNDVVEIAKLVEPSDRGELEITSINSEYLKRGKLKVELLGRGM
                     AWLDTGTHKGLLDASNYVEVVQTRQGLYIACLEEIAYTKGYISREQLMKLAKPLIKTE
                     YGQYLLKLASEDM"
     gene            34619..35176
                     /gene="rfbC"
                     /locus_tag="KR250_001273"
     CDS             34619..35176
                     /gene="rfbC"
                     /locus_tag="KR250_001273"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HBH0067232.1"
                     /translation="MGKINVIKTKIEGLYIVEPNVFYDERGYFMESYNKKDFIEQGLD
                     MCFIQDNESSSRKGVLRGMHFQVKYPQGKLVRVTEGEVFDVAVDLRKDSQTFGKWEGV
                     ILSDKNKKQFYIPEGFAHGFLVLSEKAVFSYKCTNYYYPEFENGFIWNDKQISIEWPL
                     KGIEEIFLSEKDKKQKSFKDICTRR"
     gene            35214..36197
                     /gene="rfbB"
                     /locus_tag="KR250_001274"
     CDS             35214..36197
                     /gene="rfbB"
                     /locus_tag="KR250_001274"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HBH0067233.1"
                     /translation="MKKILITGGAGFIGSNFIHYMLNKYVDYFIVNLDLLTYAGNLET
                     LIEIQDNSNYKFVKGDISNREQVYKLFEEERFDIVVNFAAESHVDRSVENPDLFIKTN
                     IIGTQVLLDASIKYGVKRYHQISTDEVYGDLPIDRKDLFFTEQSSINPSSPYSASKAS
                     ADLLVSSYYRTYGLLTTISRCSNNYGPYHFPEKLIPLMILNALENKQLPVYGNGENVR
                     DWLHVYDHCTAIDLIIHKGNIGEIYNIGGHNERSNLEVVKMILNLLGKSEELISYVND
                     RPGHDLRYAIDATKIENELGWKAKYDFDLGIKETVKWYIENESWWKAVLSV"
     gene            36227..36895
                     /locus_tag="KR250_001275"
     CDS             36227..36895
                     /locus_tag="KR250_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HBH0067234.1"
                     /translation="MNEISSLINILALTQLSGSNTTNQCNCTYNNSSGFDMIMMTLLK
                     ALSQNNQHTSNSYNLLSNSENLFNELDNVVNNTTSRFIDVDTKDKNVKLRIENAVDQA
                     SKKYNVDSNLIKAIIKVESDFNPNTVSSAGAKGLMQLMPENCRDLGVTDPFNIEQNID
                     AGTRHIKEYIDMFGGSIEMGLMAYNGGPGRMKSRGVESISDLYKMPKETQNYIPKVMK
                     YYRG"
     gene            36904..37332
                     /locus_tag="KR250_001276"
     CDS             36904..37332
                     /locus_tag="KR250_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaaR family protein"
                     /protein_id="HBH0067235.1"
                     /translation="MGINIDKLNISPSINMEVKKLKESNKFSDKLNSLKQSSSLEDIK
                     RNLESVKKAGERLVLTQNYGDISIYKNAVKEYLKSVVDNMYSLDKNSSFWEHQYYKNV
                     KIVDSKLEDMTSKLISEEKENIDIVSTVDMIQGLLIDMYR"
     gene            37356..37670
                     /locus_tag="KR250_001277"
     CDS             37356..37670
                     /locus_tag="KR250_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HBH0067236.1"
                     /translation="MNSKKEDIYELNFGQLIEEKSGVIEENQNVFNAKVNVAVSIGNT
                     TETIKNILDLKEGSIIKLSKNVDEKLDIYANDRLFAYGESIITNERLSVRLSKIEDPD
                     VN"
     gene            37822..38097
                     /gene="flgM"
                     /locus_tag="KR250_001278"
     CDS             37822..38097
                     /gene="flgM"
                     /locus_tag="KR250_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HBH0067237.1"
                     /translation="MDIKNVSSNIVVNNYLKNTSGIKKVEEVKKINKTDKIELSKFAK
                     EMASVSNMSDENIRNKKVDEIKNALENGTYKIKPKEIAKKMIEDMRG"
     gene            38102..38518
                     /locus_tag="KR250_001279"
     CDS             38102..38518
                     /locus_tag="KR250_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HBH0067238.1"
                     /translation="MITDLKVIIYEEKNIISKMMELLKEQFDYIVEKDLENLNMLNPK
                     LEDISRELASIEIKRRQLFGDDISMSEVVENSNDGYLKEIYTDLKHILALAKNQQESN
                     DSLIKKELIFTKKMINFIKPADKQTTTYNSYGNIRK"
     gene            38536..39846
                     /gene="flgK"
                     /locus_tag="KR250_001280"
     CDS             38536..39846
                     /gene="flgK"
                     /locus_tag="KR250_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HBH0067239.1"
                     /translation="MAGLFSILNTANSGMNAHQKAIQTISHNISNLDTDGYSRQRTEF
                     ATNSPMYMPSLSNSIARGQLGTGVHVTDVTRARNSFYDYQFRAEAHKYGKTVSKYDYY
                     NTIETILNEPSDYGISAGIDDFFKGWESLSKDINSDSKKSLVVENASNLATLISESYK
                     KLDKLKEDIDSNIDTEIKQINDMLKSLEDLNKSIDIISGSGSTPNDLLDERDRILDNL
                     SFKLDFENSDVKNMLADGKLELNELKNPDGTWKTGISGTLQGLFEMHGKIDTYKSDLK
                     DVSDGLAKQINDVYNTSAGITVTDFFITSNVDGEDIIKINPAIKSNPNELKLTADEAS
                     KIAKLKDEKIDIGVAGGKVSTISDHYKAFAESIGLDSQKVNQDEANQRKIINNIDNSR
                     MSVSGVSLDEEMTELIKIQRSYQASAKVMSTAVQLLDVVINGII"
     gene            39865..40797
                     /gene="flgL"
                     /locus_tag="KR250_001281"
     CDS             39865..40797
                     /gene="flgL"
                     /locus_tag="KR250_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HBH0067240.1"
                     /translation="MRVSTGMMSSSYLNSLQDNLQRLDKVNRQVNTTKEINKLSDNPY
                     KAIKILNSKSEIKTMETYVENCKDTGNWLETTDTALDQLGKLLSDIKKTLVASGDGAY
                     SDDELKTLSNETNEKMKEIANALNATHEGKYIFSGSNTGVPPVECIDNADGSVSLKFN
                     SSLNLNKLNDSLSMDIAQGISVDYNVKLSSLGFDPTKTDPFEELNNISKKLANPDADA
                     IKELTTTCLGDMEQLIENTVNVRSIYGTKANTVEAMKEKNDDSLIQLKDILSQNEEID
                     YGEKLVQLKAAELTYQASLQTGGKLFNVSILDYI"
     gene            40813..41205
                     /locus_tag="KR250_001282"
     CDS             40813..41205
                     /locus_tag="KR250_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HBH0067241.1"
                     /translation="MMKVTLKKGILGFENLKEYELLDIEDNDYLKEFNSTEEEGIGFV
                     VVSPFEIINEYEIVLNQETIEKLEVKSPKDVVLFNIITLGQTFEESTVNMKAPIVINI
                     KTNFGMQIILQDDKYSIRHPLLRGDSGC"
     gene            41199..41411
                     /gene="csrA"
                     /locus_tag="KR250_001283"
     CDS             41199..41411
                     /gene="csrA"
                     /locus_tag="KR250_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HBH0067242.1"
                     /translation="MLVISRKKDEAVLIGESIEVKIVGVDGNNVKLAISAPNNISILR
                     KEIYEKVKSENIKATNKNIKILKSLK"
     gene            41433..41828
                     /gene="fliS"
                     /locus_tag="KR250_001284"
     CDS             41433..41828
                     /gene="fliS"
                     /locus_tag="KR250_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HBH0067243.1"
                     /translation="MYGENPYNSYKQNAVFMASKEQLLLMLVDGAVKYTKIAKTAILD
                     KNTQKAHRELIRVQDIFTELMVTLDQNAGQWAKDMYRVYDFVRYELSRANIRKDIQII
                     DNVLPVIEQIKDTWHEADKKSKEERSRYK"
     gene            41828..42184
                     /gene="fliS"
                     /locus_tag="KR250_001285"
     CDS             41828..42184
                     /gene="fliS"
                     /locus_tag="KR250_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HBH0067244.1"
                     /translation="MELNLAELDKLSKEELLLMLVDGTVKYTNISKEALLNNDYLKAH
                     NELVRVQNIFTELMTTLDQDAGQWAKDMYKVYEFIKSELAIADENKDIKIIDDILPIV
                     KQIRDTWHEVYNQLKI"
     gene            42205..43728
                     /gene="fliD"
                     /locus_tag="KR250_001286"
     CDS             42205..43728
                     /gene="fliD"
                     /locus_tag="KR250_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HBH0067245.1"
                     /translation="MSSISPIRVTGLSGNFDMEGIIEASMIRDKEKVDKAKQEQQIVK
                     WKQEIYRNVIQESKDLYDKYLSVNSPNSIVSEKAYSSTRITSSDESIIVAKGSAGAEK
                     INYQFAVSQMAEPAKFTIKLNSSEPIVQQFPPNASGASSLTIGDVNIPISEQDTTSTI
                     VSKINSLCADNDIKASYSEMTGELIISRKQTGSSSDINLRVIGNDNLAQQIANDNGIT
                     FINDAGGNKVANVYGKNLEADVTDEHGRVTHISKEQNSFNIDNIDYNVNSKGTAKLTS
                     VTDTEEAVKNMQAFVDDYNKLMDKVYGLVTTKKPKDYPPLTDAQKEDMTTEEIEKWEK
                     KAKEGILRNDDELRGFVEDIQSAFFGDGKNIIALRKLGINESENYNKKGQISFNADTF
                     SKALIDDSDKVYKTLAGYSSNYDDKGMFEKLKDIVYEYSGSSTSKLPKKAGIEKTASA
                     SENVYSKQIAEQERNISRLVEKMNDKEKRLYAKYSALESLLNQYSSQMNYFSQAQGN"
     gene            43731..44066
                     /gene="fliT"
                     /locus_tag="KR250_001287"
     CDS             43731..44066
                     /gene="fliT"
                     /locus_tag="KR250_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliT"
                     /protein_id="HBH0067246.1"
                     /translation="MDKLKEKLNLYKDISLQIINLIEKEEYIKISIKLGERQEIINSV
                     SEIDRNDFIQLYNRMELIEIDSRIRDILQGQLLEVKKELHEYKLTKQVNTMYYNLNRE
                     KVNIFNKKV"
     gene            44168..45040
                     /locus_tag="KR250_001288"
     CDS             44168..45040
                     /locus_tag="KR250_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HBH0067247.1"
                     /translation="MRVNTNVSALIANNQMGRNVNAQSKSMEKLSSGVRIKRAADDAA
                     GLAISEKMRAQIKGLDQAGRNVQDGISVVQTAEGALEETGNILQRMRTLSVQSSNETN
                     TAEERQKIADELLQLKDEVERISSSIEFNGKKLLDGSSTEIRLQVGANFGTNVAGTSN
                     NNNEIKVALVNTSSIMSKAGITSSTIASLNADGTSGTNAAKQMVSSLDVALKELNTSR
                     AKLGAQQNRLESTQNNLNNTIENVTAAESRIRDTDVASEMVNLSKMNILVQASQSMLA
                     QANQQPQGVLQLLG"
     gene            45133..47274
                     /locus_tag="KR250_001289"
     CDS             45133..47274
                     /locus_tag="KR250_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBH0067248.1"
                     /translation="MLLSIVMIVKNEEKILEKTLKSLTTLRNSIESELIIVDTGSTDD
                     TIKISKKYTEKVYFHNWNNDFSSMRNISISYAKGEWLLILDADEILIDSSTIVKFFKD
                     GLDKKFNSASIQLKNLYSYDKKLYGYCSVLRLFKNVDFRYHGKVHEQPIYKNPIFNNV
                     ADFEHYGYLFEDEEIRLNKVKRNEKLLFQELKENDNSPYTNYQLGKNFIILGKYQEAL
                     YYLEKSYELYSKLELAPGYLITSLVKTYLYLGKHKKCERLCLKYIKKDMNNIDIYYYI
                     AQAQVDLGKYENSIDSYKRYIYLLDNYEISTQANSLFSDTDTVGLRDEAIITLIKIYY
                     KLEKYEFVISEYNNIEDVEKKKNVYFSLFMSLYKLNRFEDIKNYYKEVSNSKIEKNSF
                     YRNIEEVINNIKEDEKEYVYKILCDIEGNYGILNQIRINKCVSIDKCKEILNEEKEII
                     YAYIIKIAFEEKIDLLDIFYEMEYVWIEKYLKYLLALERSFNLVLYKYIISRPNTSDM
                     EKIRVYKAISKVVLESISLSDEKYKEIFYLYIMYSYKYIRYIYSNLSDFELIKFVCND
                     LDKFTIEFKHLLDIKNKVNNANEKLEYIHSMRKLLNEYPFYNKIIKILIGELEENIKE
                     SEEFKDLKDSFIISIENMIETGKINDAKLLVDDYSKTFVDDIKILNIKGILYMFENKL
                     DKADFMLKKALSLDVENEDTIYNIEYLKSLR"
     gene            47298..49175
                     /locus_tag="KR250_001290"
     CDS             47298..49175
                     /locus_tag="KR250_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBH0067249.1"
                     /translation="MNIPIVVNEKSKDIIKDWQYTCTNEDIKFTSIIILTYNQIEYTK
                     LCIESIRKFTPKNKYEIIIIDNNSSDGTVEWLKGQSDIKSVYNNKNLGFPKGCNQGIE
                     LATGENILLLNNDVIVTPNWLYNLNKALWSQDNIGAVGPITNACSYYQQIDVDYKSIE
                     DMFIFAHKINKSNINLWKYRLKLVGFCMLIKKTVLDNVGVLDELFTPGNFEDDDLSFR
                     MIESGYKLLLCQDTFIHHFGSVSFKENRNKYTDIMYINRRKFETKWGFNPEYSGMIRT
                     DLISKIDDSLEANINVLEIGCGIGATLLEIKNIYKNASIYGIEISESAGKLAKYSADV
                     LIANIEKVKLDYKKDFFDYIILGDVLEHLIDPWKIVNELKQYLKKDGYLVSSIPNIMH
                     VSVLRNLVLGNFTYEDSGILDKTHLRFFTLNEITKMFNDSNYIINSITATSMPLSEID
                     NSFIDTICKESCKEMRQQYESYQYIVKASKILDTKRYSSISMINLKYALMRIDNELDV
                     DSNLSYIFDNYNITEPCFVDDIEYLIDTFVINKATLLNRLGVEAFNRGLGDFSINMFM
                     TVLEMDRDDIDSIYNITSVLCELEEFDTAYNLIKNSSEKVQSESDIQEILCFIEGKKY
                     E"
     gene            49168..51282
                     /locus_tag="KR250_001291"
     CDS             49168..51282
                     /locus_tag="KR250_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HBH0067250.1"
                     /translation="MNNDKICFITCVNDERLYEECLVYINSLDIPKNFSIETVALRGA
                     DSIASAYNKAMRETDSKYKVYLHQDTFIINKNFIHDILEIFKKNTDVGMIGVAGVKDM
                     PVNGIWWDSDKKAGKVYDSHTGKMDIYEFSNFEECFNEVMAIDGLIMITQYDVFWRED
                     KFDGWHFYDLSQSMEFRQNKYKVIVPKQNKPWCMHDSGVVNINNGYDEYRIKFVKEYI
                     NLDLYRENFYEFDKKKASTILKSNKHVMDCMKEELTEQLRLKNYKDVLGIVSKIAHFS
                     VFNHTGLYSCEECERALLECANNLPEIENTKLFTKKDLKRRKVLHVLSEGYSTGGHTR
                     LVKNWIEIDKDSVHSVVTTWQSDTLPDWLLKAINDSGGEVISLDLISDDYLKRAVALR
                     KIAYDWADIVILYVHMWDTIPVVAFGVDGKIPILHVNHYDSTFWLGTSIADIVIDFSE
                     TSQLITLDKRGIDKSEILPMPLKEKEVFSKSEVRKRYNLEDDVTIILTIASERKYNSV
                     NDINYFKIAKTLIENTDNTIIIVVGVDTENLYWKTLSNVTDNRVICFGTTANIDDFYK
                     ISDIYLESFMVGSHTSKLDAILYDLIPVKFKNEVSPIFTNMWNFIDKFDYKNIKEVID
                     MIRRYNTSEFDRDKIKAKQSEIKHFVIDKHCIEIKNYIDKIYNEVNHHKVKYDLYKND
                     EIEDYQLFVALYGYMVKNKNMS"
     gene            51291..52286
                     /locus_tag="KR250_001292"
     CDS             51291..52286
                     /locus_tag="KR250_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HBH0067251.1"
                     /translation="MNNIHINKFEKSCEKNWDYFVENKSINGTFLQTRNFLNYHEEGK
                     FTDHSLIICKGEEILAVVPACETYEDGERIFFSHLGSTFGGIIIRSGFNDIRHVNAIT
                     NKLEIYLQEQNFDKVYLKNTSRIFCKEDTSLLEYFLFKNNYMFYDELSMFLELSKYKE
                     DILANFKYSKRRDFKYSLKNNLTFKRLYSDEDIVCFFNLLSNCLDSKGAKVIHSVEEL
                     LEFKNNRLKDIVEFYAVYIDDIMICGSMVFNFQERVYHTQYLASNSTYNKYYPMIFLY
                     YNLIKDALERNFKYLSFGISTLNRGKYLDEGLATFKEGFGSSAICNRTYYKNINK"
     gene            52378..53610
                     /locus_tag="KR250_001293"
     CDS             52378..53610
                     /locus_tag="KR250_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HBH0067252.1"
                     /translation="MKRLMILGSIKYFENIVMSAKREGIYTIVCDNRIDTPAKKICDE
                     AIDVDVFDFDKIKNIAIDKKIDGILTGFTDSLMKPYVYIANELNLPCVISCNQLESVT
                     NKAVMKNYFKNNGIPTTDYCIIESLKDLEKIQGLNFPLVIKPVDGYGSRGVYFVSNIE
                     ELIEKFEYSKEASQDGQVIIEEFYESEEIQGLAWVHNGESHVFYIGDRELVNIHTGRA
                     GKPDRLIYPSKYCFQYEEEIKSIYQNITQAFGIKNGPLYVQMLVGAYGIKVSEVMPRL
                     PGGCDYMAISQITDLDIGSLFANFSVNNEVNFDEIKKYNMHLSKCVYALPIYIRPGTI
                     KKINNIDKIENLDYVTQFLLLVKEGDTIGLYEDERQDCGRVYGVANNVFDANIKKKCI
                     HEMIEILDENNENMIENF"
     gene            53630..54574
                     /locus_tag="KR250_001294"
     CDS             53630..54574
                     /locus_tag="KR250_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase"
                     /protein_id="HBH0067253.1"
                     /translation="MKLITHEQIMSLNISQKQGYEWIKESFLTKKDCQLPPKISMVEE
                     GHVFYNVMPSLNHNHKIGGIKMVTRLPGRNPSLNSEIFLYNMEDAKLKAILDGNYITA
                     FRTAAVAALAIDTFAKKDFNTIAIMGLGVTASAIMNMLSYTLKDKNINIKLLKYKDQA
                     ESFVKKYEKNEQFSFEICDTHEELIVDSDVVISTVTYTDEYLSEFKWFKKGALLLPVH
                     VMGFGNVDYLFDKVYLDDYNHLKHLENIDRYKYSCEFYDVLSKQAKGRENDDERIISY
                     NIGLSIHDILFANKIYELCETLSCEEIELKEPKEKYWI"
     gene            54668..55075
                     /locus_tag="KR250_001295"
     CDS             54668..55075
                     /locus_tag="KR250_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxcM-like domain-containing protein"
                     /protein_id="HBH0067254.1"
                     /translation="MDKYNLIRFVSIGNEYIGNLVALESNKAIPFDIKRVYYIYDVPN
                     NVKRGFHAHKNLEQVLVCLNGSVKIKCYDGVKETIFNLHKNDVGLYIGTGVWHEMFDF
                     DEKTVLISIASEHYNEEDYIRDYKEFLKYLNNV"
     gene            55162..56253
                     /locus_tag="KR250_001296"
     CDS             55162..56253
                     /locus_tag="KR250_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HBH0067255.1"
                     /translation="MLVKFNNLLKQFQTHKEEYEQKIKDVMESGYYILGNEVKHFEEE
                     FANYVGSKYCVGLASGLDALIIAFRALGISKGDEVIVQSNTYIASVMGISINGATPVF
                     VEPNEYYNIDSSKIEEKINSKTKAILVVHLYGQASNMKDIQELCDKYNLYLIEDCAQS
                     HGAKYDGKMTGTFGDIGCFSFYPSKNLGAFGDAGAIVTSNEEIADKVKILRNYGSEKR
                     YYNKFVGYNSRLDEIQAGLLRVKLKYLNEIEIEKSKIVDRYLNEINNPKVILPKIENN
                     CTHVWHLFVLQVEKRDTFMEYLKKSGIESIIHYPIPPHLSEAYAYLGYKKGSFNIAEN
                     YSDKVVSIPLFIGMEDEEVSYIIKIINEY"
BASE COUNT        22379 a         5955 c         9902 g        18127 t
ORIGIN      
        1 aattgtttga aaatttaata attttataga tatgaaagtt attttcttct aagaaaacag
       61 actaaatgcg tagaatattt ggtaaaactt acctgtataa attgaaatca gaaggtttta
      121 ttattgattt agattagaac aatttgaggg agtgacataa aatgaaacaa caagaagtag
      181 ttaataaact acgcagggga gaattgatgt tgatggaata cttgtggagg aaaaattcta
      241 ttttatctaa aaaagaaata atagaagcaa tgaaagaaaa atataagtgg aggaaaagta
      301 ctacagaaat tctattaaaa agattagtaa aaatgaaaac attaaaaaag aagaggatag
      361 gttttcagtt tagttatgaa gtgctagtaa ctaaaaaaga atatttaaat gcaataaaag
      421 aagatagaaa ggtaactaaa tatgatagtt tttttatacg aatgtttacc actatgcata
      481 aaaaagaaaa gatgacagaa gaacaaataa aaaaatatat agaaaatatg gaaaaaattg
      541 gaaaagaata atttatatat atattaatta tttttaaaat aaatttgtta taatatttaa
      601 atatacataa acatacataa atttacaaaa agggggtgta gcttatggga ttcaagaaat
      661 tgccagattc tgagttaaaa gtaatgaagt ttatttgggg attagatgga aataaggtag
      721 catcaggaga agttgtggaa gcaatgaaac aaatttatga ctggaataaa aatacgacat
      781 taacaatatt gtctaagctt gcaaagaaaa actatttgta ttctcaaaaa acaagcaagt
      841 gtacatatta tactatcgct gtaagagaaa aagattattt aaaagttgaa acaaaaaaat
      901 tctttagttt ttttcataat aattctttaa aaagtttttt cactgcttta aatgatgaag
      961 aaaatttaag tgatgataaa ttagatatgt tagaggaatg ggtgaaaaat tgggaagaag
     1021 atgaataaaa tgaatgtgat actaaaatga tactaatatg ttttatagcg tgttaaaaaa
     1081 tatagaaaat atagaaataa tagaagaaaa ttaatgtaat agataaggtt ttatcagatt
     1141 aaaaagaaaa tcaatagaat gggaataaaa tatagaatgc caaaaatcca cataaataca
     1201 ggatttgagg tctctcttac ttctatgtta tactggtttg atactatcaa tatttgccag
     1261 tatcttatgg attaataagc aaaaaacatc ctgctgaatg ataaattcag cggggtgttt
     1321 ttattttgtt ttagaactcc tttgctctga aactcgaagt atttaacact gatgattttg
     1381 ttatctttta gttccttttg tgtcatgctc tgaaagacaa attgatacaa taaaaaagta
     1441 atttcttaat cattgatgga gatatgtgtt tatgttactc ttgatggtaa tttgagtaat
     1501 attgttcata gctttttata ttcacttaat tagaaaacca tttggtataa tcataaataa
     1561 acttgatgat ataatagaaa agatttataa ttattagaag tatataaaag tattttatgt
     1621 ctatgaaatg tatgtctata aaacatatag atgacttttg tatattaatg tatgaatatt
     1681 gatattatag aagaattgag ttgtcaatga tactaaatca aatatttata gaagctggag
     1741 ggattatgag aaataaaata attataggaa ttactttttc acttgtatta ctaatgtctg
     1801 gatgtgcttc aaaacaaacc gcagaaataa acgtattagc aactactgat ttacatggag
     1861 ttatacctta tgagttaact tcttatgtaa aagaagaaag aaaaaaagat aaaaatctaa
     1921 ctttagtaga tgcaggagat ttttttgata gtggagaagt gtttggttct agcatggata
     1981 gttattttag tgctagatat aaagatggtg ataataacac tgaaagttat attgagacgc
     2041 ctatttcaaa agatatgaaa gaggtaggtt atgatgttgc agttcttggg aatcatgaat
     2101 ttatatccaa caataaattt tatctagata atatgatatc tgattttgag aagcaaaaaa
     2161 taaatatttt atcggcaaat acgtataaaa aaaatggaga tagttataca aaaccataca
     2221 taataaaaaa tattaataca cctgaaggaa acgtaaaact aggaatttta gggcttacta
     2281 taaaagaagt tggtgagagg aaacagtgga aagatggaaa acttgttgac tctaagtctt
     2341 tagaactaaa agaccaagag ggttacaatg gtgaattgta tatgaatgat ttagtagaag
     2401 atgctaaaaa gtgggttggt ataatggaaa aagagaagcc tgacattata gttgctgtgg
     2461 cacatacagg tgagaaacct aagaaaccta aaaacccagg aaatagaata caagacttag
     2521 ctcaacaagt tgatggaatt gatgctatag ttgcaggtca taatcatgtt caaattgagc
     2581 aacatgatta taaaaataaa tcaggagaaa atgttatagt tactgagcca ggaaagcatg
     2641 gggaatgtat ttctaagatt aattttaaat tagaaaaaaa caatgataaa tggaatatag
     2701 ttgacaaatc tagtaaatta attaaatttg aaaaaaatca agaaagtgat aaatttggag
     2761 aatttatttc ttcaattaat aaagtaagta aagataagaa agaaataaga ttatcagaag
     2821 taaagactcc tgaatgggat aaaatatatg tttttaaatc tggaacatct gcagatgata
     2881 tatataaaac aataggatat aaatggagaa atatatcatc tccaaagggt gatgacatgg
     2941 tgcagattgt tgtaatgaat aataataagc ctgttagcta tgtatatggt gaagctgaag
     3001 ttatgccaat taatataaaa tgtgatgaat cagagtataa agataatgtt gtaactattt
     3061 atcctaataa aaatgataaa tttaaaattg aaaaaggtaa acaagaatat agtattaatt
     3121 taaaacatat acaatagaaa cttttatctt atttaagttt agattgtact taaaataaag
     3181 tgtgtattat tttaaaaatt gatgataatt aaaatcacaa cattaaagaa tagtacacac
     3241 tttttatgct aaaaaataga atgttaaaaa attaaacctt taaattaaag tacattgctt
     3301 atattatcca atattatttt caaaggttgt gtaatatttt tatccttatg taatattgct
     3361 aacattttat tttccatatc tatcccatct aattcaatca atcgcaattt tttatgtaag
     3421 agtgaatcta ataataaatt ttctggcaaa atggttatac ctaaccctgc ttgtgcagct
     3481 ttaataagtg caaaagaatt aacactttcc caaacaggat aagctttttg gtttgaaaga
     3541 gataatacac tgtccaaagt attgcgaatg gcacttcctt gctcacgtaa caataacgga
     3601 aacgtgcata actgtcgtag agataaggct tgtttagtat tggtggtggt tatgcaatca
     3661 tacttttaat ccaagagaaa gtagtaaatc aaactggatg gataagtgag aaaatgttta
     3721 cagatattat aactatttca caaatgacac cagggccact ggctgttaat acttctactt
     3781 ttgttggact tcaaattgga gggttttggg gagcagttgc agccacttta ggttgtgtat
     3841 tatgtggtgt agtaatatct ctatttttat ataatttttt tcaaaagcat cagaactcta
     3901 cttatatatt tgaaatatta aatggtctaa aatcagcttc attaggttta attatctcag
     3961 ctgcggctac aattataatc cttgcattat ttgggacgaa taaagtgtac ttcaattcta
     4021 tatttgagat gctaaactgg actgcactaa taatcttttt tgttacacta ctgatagtac
     4081 gtaagtggaa aattaatcct attgtgatta taataataag tggtatactg ggaatatttt
     4141 tttatttata agtttttggt aagtagagga tatctatttt tttatatctt aaatccaaat
     4201 agccaataaa gtaaggccat ttggattttt aataaattct gaaaagataa tcagaaaccc
     4261 atataacatt taaattatat cactggtttt ctacaaacca atcataagct tcttggcaaa
     4321 atttctcact atctatatag taactattat gaacattatt aatttcaaca agtttctttt
     4381 tacattttag tgaattataa tagctatcaa aatattgggt tttaaaatat ctatcatgaa
     4441 gtgttttaaa gacaagagta ggtatgttga ctccatcttt tatcttacta tcttttgcaa
     4501 taaagcattc taacattgta ttaattatct ttgtactatt tttccaaata tgaggatttt
     4561 tcttttcaaa tattttataa aattcattat catagacttc attataacct ttatatttag
     4621 gtggttttac tgttatgtga aaattaggaa gaaacttaaa gaaaaagttt aataatttaa
     4681 cagtgagata ggtatagcct actcctgttg tgtttttata atcaacacaa gcaaactgag
     4741 caaaactttt taacttattg tctgaacatg cataatattg tgcaaacata ccaccaattc
     4801 ccgtattccc atatagatgt atgttggaag aataattttt ttctatgtat gtaatgacag
     4861 aatctaagtc ttttacaatt gaattgcgag aaaaatcttt ttcatatcca tcacttttcc
     4921 ctgttcctga aaaatcaaaa gcaaatatat ttacattatg tttggcaagt ggttcatatg
     4981 ctttttgcat ctcaagcgta gtgacaatag gagtatgtgt aacaacaata ttcatggatg
     5041 attttctggt aataaatgag ttacaaaaaa tcattgtatc tccatttggt atttttatat
     5101 gtttccaact catttgattt tcctttctct attataaaat ataataattt ttatgttctg
     5161 ttttatatat tattcatatt ttatcacaga taatatacta aagttaattt ttttattgta
     5221 cacctattat aataattctt ctataatata agacattcta taattttata tttaagttca
     5281 tgtgtattta attgttttta tataaaatgt ccagtaatac tatttggatt tatttttatt
     5341 tcatcaggag ttccagaagc aacaatttcc cctccattta ttccactgtc tggacctata
     5401 tcgataatcc aatcagaagc acgtataacc tctgtattgt gttcaataat taaaagtgaa
     5461 tgctgttcat ttacaagttt tctaaaagta attaacaaat tttctatatc tttaaaatga
     5521 agtccagtag taggctcatc caatatgtac agcattcgtt ttttagtttt tgtccctaat
     5581 tcttttgcta attttatcct ttgagattct cctccactta atcctaatgt attttgtcca
     5641 agctttagat atccaagtcc cacttcatct aataaggaga gttttctata tatatcagag
     5701 caatcgctaa aaaatgtttt tgcttctgct acttccatgt caagtatatc tgcaattgtc
     5761 tttcccttgt attgtataga cagtatttct ttttgatatc tcttaccttg acattcacta
     5821 caagttaccc attggtctgc catatattgc agaataattt ttgttttacc taatccttga
     5881 cagtttgggc attgcccctt tttgctatta aagctaaaat agctttcatc caataatttt
     5941 tgttttgctt gtggagtttc agcaaataga gaacgaattt catcaaaaat attgatatat
     6001 gtagcaggtg tagaacggct agattttcca ataggtgttt ggtccattaa gataaaatca
     6061 tcaatagcat caaatccagt aaattcagta taatttttat ctgggataga tttttgtttc
     6121 aatttttctt ctagagcagg aagtaaactg tgaaatacaa gagagctctt accagaacca
     6181 ctaacacctg taatagagca catacagttt aagggaattt ctacatcaat attttttaag
     6241 ttatttgcat ggcatccttt taagcttaac catttacttg gtataatttt attttgtgaa
     6301 cttattttat tgtatgtgct taaatattta cctgttatag agtttggatt ttttataatt
     6361 tcttgggttg taccttctgc aataatgaac cccccttttg taccagcact tgggcctaca
     6421 tcaataatat aatcagcaga cagtattgta tctttatcgt gttccacaac tataacagta
     6481 ttacccttat ctcttagctc ttctatcatt ttgatgatta aattatggtc tcttgggtgt
     6541 aaaccaatag atggttcatc taaaatgtag gttaagccta ctaattcact tcctagctga
     6601 cttgataatc gtactctctg cagttctcct ccagataaag ttggagctgt tctatctgtt
     6661 gatatatagc taagacctac ctttaataga taactaactc gaatttttaa ttctgttaag
     6721 atatcattta ccaaagagag ctcatttttt tgaagttgat ttggtaattc acacaaccaa
     6781 ttccataact gttctatcgt catttttgtt agttcaggga agcgataacc tttgattgta
     6841 gtgaatctag cctcaatgca cagcaattcg ccaccacatg tattgcaagg aattttgttc
     6901 atatattgat gcaaggtatt attttcagaa ctagattctc taaaaagtct ttgaatgtgg
     6961 ttgattgctc ctgaagcagg tcttctaatc tttgattctc gtccctttga ttcaaagcta
     7021 aattcatata tcttatcgtc tgtaccatat aagattgcat ctataaaatc tcttggtaat
     7081 tcattccaag gtatatctag gtctatattt ttatcttttg ctattgcata taattcacct
     7141 accatccaat taccatttgg ctttttcccc ttcaattttc caaaatagta agtagctcca
     7201 tctaatattg aaatagttgg gtcttttaca atcaattgag gattaacttg ataggtaaaa
     7261 cctaatccat tacaatcgtg acaagctcca gtatgggagt tagcattgaa ggttgaagta
     7321 tttatcttag aaaaaatagt ttgacaatgt ggacaataat tttttgtatt attctttttt
     7381 tctaatggaa ttttacaata tggacagtaa ctttttccga ttgatacaaa taacattctt
     7441 aaataatccc aaatgcttgt aagagtacca acagtagagc gaggattttg gcttcctttt
     7501 ttctgttcga tagctatagt aggggttaat ccctgaagta aatcaaattc cggtttttga
     7561 agctgttctg aaagttttgc tccattactt agattttcaa aataacgtct ttttccttct
     7621 tcataaatcg tatcgaaagc taaagaagat tttccactac cacttacacc tgtaataaca
     7681 gttatttgat tttttggaat tgtaacctca acatccttta aattgttttg ccttcctcct
     7741 tttaaataga tagagctttt attatgacaa agtgaatttt taattgattt atttattgat
     7801 tttgtaaatt tgaatttctt ttcgagttca agaggattgc ctaattcttt tatttccgaa
     7861 atgcctaata tttttaactg agaaaagatt gatttcaaat aatcttcagg caatttttct
     7921 attaacaaat taaggtcttc tatttttata tttccgcttg ataatatttc tgccatttca
     7981 tctaataaat ttttttctgc tcttgttaat tgattgtatt ttttggaact taattctgtc
     8041 cataaatccc atggaagcaa ttctaaacca atcatattat taaagtcatg tagaagatta
     8101 ccctttacag aatgccaacc tttccaattt ttattatatc caaagtcatc ttgatgagca
     8161 tgtccttgcc gacatcttat ccaggcttct gaaccagtta aaaaaccatc tgtttttccg
     8221 acatcatgag tgtcaaaatt aatttgaaaa taattttttt gtatgtcatc tatttgtgca
     8281 tctattctta tccatctctt tgttagagta tcatagtatt ctacaagcca gtggtcttca
     8341 taagtaagct cactttcaaa ataattagca aaacctgctc tcattctagc ctcaaaacct
     8401 cgatatctta ggagcgatac aagaagtaaa gaataatctc tgcacattcc aacaagcctt
     8461 tgttttgggt ttcttggaat agttagtggg ttagaattta tttcaaatat tctttttaac
     8521 atttgaggaa ttgtacgaag caattcctca tcagattgtt gtttattaaa ggatatacca
     8581 tataattttc cttggtctcc atgaattact aggctttgaa ccacttttaa taactcaatt
     8641 ggagtctttg gtaaatcttg atatttattt tcaaattttt taggatttgt gatgatgctt
     8701 tgatttttat agttatttat tttcatttat atttcacctc tctaaatgga tgatataccc
     8761 tgacatggtg tcatatgcaa gggcaattta aattttttgg ccaaaattta aaaataaata
     8821 aaaaatgata cagtgataaa ttgtaataca aatatagttt caaattgcat ataatataat
     8881 tatttaataa aaccattatc tatggaatgt taatgaaatg ttaagatggc attcttgaat
     8941 attaagaaat agaatactat aatatagtgt gaaggatttt tagtaaaaaa atttgtaagt
     9001 gttaatatac attaaaaaat agatttataa tttggaggaa aaaaatgaaa ttatcaaggt
     9061 ataagaaaaa tagtttagtt ctgtctataa ttttatgttc acttattatt tcaatccttg
     9121 taattactgt ttttaatacc gtaaaactac acaatatatc taataaacaa tctaaaaact
     9181 atttaaatga tgtatctaca cagattgtaa tgaatgttga ttctaaaatc aaatttattt
     9241 tatccgactt aagaataatg gcagatttta ttaagcaata tgaaggtgat agtcgatatg
     9301 agtatttatc taaaagaaag actagttata agtattatga tgttggaata atagatttag
     9361 aaggtaaagc tgagttttta agtggaaaaa agtttgactt aaagaatact gtttcctata
     9421 aaaaaggaat tgaaggtaaa gaatactcag aagtaataga gtcaatgggt tttgttcttt
     9481 atagtgtacc tatatttaat gataatcatg aagtatctag tattttaata ggtgtatcca
     9541 ataaagaaag tatgaatgag attcttaata tagataactt taatggaaaa ggaactattg
     9601 aaattattaa tagtaaggga aaacctcttt tcataggaaa aaatactgaa ttaataaagg
     9661 accttagcca taaatataag agttctggag atgaaccttg ggcacaaaag atgcttgagg
     9721 attttaaaaa tgataaatca gggaatatta caataacctc atcaaaaggt gtacaatgct
     9781 tattaactta tcatccaatt acaagaggtt taaatgattg gcattttctt ctcatagtac
     9841 ctgaagatgc tgtacttggt gaacttaata aattaaactc ttttacaata acaatgacat
     9901 ttataattac atgtattatt ggaattataa cattaatttt atatattata agaagaaaat
     9961 atgtaaatca aatagaaaat attgcatatt tagatagtat aacaaatggt ataaattcaa
    10021 ctaagtttag tatgctagtc aaacctttaa tttcagaatc acctgatagt acttatatga
    10081 tgatagcaat gaatataaaa gattttaagc taataaatga ttgtttcggt agtgaaaagg
    10141 gaaatatgac actaaaacat ctttacaata ttttgtataa aaacattgat gaaaatgaag
    10201 agtttgtgtg ccgtcatgat gcagaccttt tctatttgct tattaagaat agaccagtgt
    10261 tagaagccct ggagttatta tataaaatag aagctgacac aaattatttt aataaaaata
    10321 gggaaaatcc atacttttta agactatcag taggtattta cacaattgaa aatcataatg
    10381 aagatttaat tacaatacaa gaccatgcaa atacagcaag aaaaagcttt aataaaactc
    10441 atcaaagtga tttttctttt tatagtgata tagaaagaaa acgattaata tctgaaaagg
    10501 aaatttcaaa tttaatggaa aaagcccttg aaaataaaga attttttatg tgcttacaac
    10561 ctaaaattga tataaaaagt ggaaaaatta gtggagcaga gtctcttgtg cgttggaaaa
    10621 attcagaaaa gggcataatt tatccaagtg attttattcc tcttttcgaa aaaagtggat
    10681 ttatatgtaa gttagattta tatatactag aagaaacatg caaattgatt tctaagtgga
    10741 taaaagaagg taaagaagtg ttgagaattt ctgttaatgt atcacgtcaa catctgaatg
    10801 ataaattgtt tttagaaaaa tataagcata tatgcaataa atacaatgta ccaacatgtc
    10861 tgatagattt agaacttact gaatctattt tcttagaaaa tccagaagct atagatataa
    10921 tagaggatat tcattctaat ggatttaaat gttctataga cgattttgga tttgggtact
    10981 catcattagg gatattaaag gattttaaag ttgatataat taagttagac cgttcattct
    11041 ttgttagcaa aaataatata gaccgtggta aggtagttat taagtctatt attgaactta
    11101 gcaaaaggct aggtatgaaa gtaactgctg aaggaattga agaattagaa caagttgagt
    11161 tcctaaaaaa tgtgggatgt gattatattc aaggttatgt tttttcaaaa ccattattaa
    11221 tttctgactt tgaaaatttt gcatatgaaa ataataaaat taaaaatatc cattattaaa
    11281 tgtacaagat aaaaaattta ggcaagttat ataattaaaa atataacttg ccttttttat
    11341 ttatatattt aataaaaata tatttgcaga ttttgtaaaa ataattttca aatgttgtaa
    11401 gttgtggcta aagctttatt gtattattct gaatatagaa agatagttac tgtcttagat
    11461 aaaaactgaa acatacataa taatataaaa tttgctatat taataactct aattattttg
    11521 gaaggaggga aaatatgata agtgggagaa tgttactcat catgaatttt ctaaaaggaa
    11581 aaaataagac aagttacaag gatattagca aacaacttag tatagaagaa agaaaagtta
    11641 gatatgatat aaataattta aatattgttt tgcgttcatt aaacaagtca gtaatacaaa
    11701 agatgaacaa aggtgtgtta attattcctg atgacttcca agtagtatgt ataaataata
    11761 acgaatatgt attttcctta actgagcgag tatctattat gaaaattata tcaatgttta
    11821 aaatagataa tctaaactta gaaaaattaa gtaaagaatt tcaggtttct agaagttcaa
    11881 ttaaaaatga ccttaatgtt ttgtcaagtg aattagaaag aaaccaaata actattactt
    11941 atgataaatc ttttaaaata agtggagaag aagacattat atataagcaa agattgaaca
    12001 tattaaaatc gtattcttat ctttttggta aggaaaaatc tgatttaagt gtatttgaga
    12061 agtatttagt gaatgtaatt gaaaaaatat tcaaagggat ttatttgatt gatatatata
    12121 attggtctat gaatttatta ttgcaaatgg gatggacttt aaatgatgaa tcttttacat
    12181 ggtatgtgtc aaacattttc ctttttacat ggtatataca ttcagatgaa aaacatcctt
    12241 tagaatctgc ttcattaagt gagccgtgtt ttgaaaataa gtttgaccaa gaatttgaaa
    12301 caattattca aaaaaagttg actaaggaac aaatttttct gttaaagagt tttgtatttt
    12361 ttacaaataa atatgcaagt ttaaacgagg atatggattt gattagtaca gaaattatag
    12421 ttcaagacct aatttacaat atgtcagaaa tattagagat tagctttaaa gaagatatga
    12481 ttctttataa aggattactc aatcatattg ccccattgat agagagaatc aagcagaata
    12541 tacagattta tgaggaatta cttcatgtaa taccagataa atatcaatat gtacttgaag
    12601 ctactaaggt ttctattcaa aaaaacagag ttttaagtca aataaagaat gaaaatgaaa
    12661 caatactttt ggcaatacat ttcttaggaa gtatacatag aaatgagagg aataactaca
    12721 aaaatatatt gttagtttgt ggatttggat atggtgtaat tgctatgtta aaggatagtt
    12781 tagtaaatga ttaccagata aatatagtag agagtattcc atcatataag ttacaagatt
    12841 ataaagactg ggaaagtatt gacttcatta taacaacatc aaagatagta atagacttgc
    12901 ctaaaacaat tattgagata aatccatttt tacaagaaga ggattatttg aagtttgaag
    12961 aaaaagggat aaaaagaaag aatgtcttaa ctaattatat gtcaataaaa aaaaggttgg
    13021 actttttaga agaagatgcc caaaaaagag tattaagtat tatatgtcaa gaacttggat
    13081 acagtgatga gagaatatta tttagacaac ttaatttaag tgatttaata gggattgata
    13141 ccataaggat aattgatgga gatatgaaat gggaagatgc tgtaaaattt agttcaaaca
    13201 tacttgctaa ttgtgatttt gtatcagcac aatatgctga gaatattata gatattttaa
    13261 aaaatgtggg tttttatgct gtaaaagata acgaatttgc tttactacat ggcaatgata
    13321 gttcattagt caaagtaagt tcgataagtt tattaatatg taaagaagca gttagttttg
    13381 gagataaaaa ggttaaaatc atattttgcc tagcaagcag agataaaaaa gagcaaattc
    13441 ctgcaataat taatctaact aaaatggttt ataagactaa ttttattgct atgttagaaa
    13501 gtgcaaagac aaaagaagaa gcagaagttt taattcataa atatgagaag gaggtcactg
    13561 tatgaataaa ttagttaatg taaaatgttc atttaaaata catgttaatt caaaagaaga
    13621 agccattata tcgttagttg atgtagtgtc aaaagaagga tatttaatag ataaaaatca
    13681 atttttaaaa gatgttttaa agagagaaga aacattgtcg acttatattg gacatggtat
    13741 aggtcttcct catagtcaaa gtgtaggtgt taaaaactct tgtattacaa taggaaagct
    13801 tgatactcct attgaatgga cagaagaagg agaaaaagtg gatttaatat ttttgatatc
    13861 agtaacaaaa gacaatgaaa ataatcttca tttaaagatt ttatcaaaat tagcacgttt
    13921 attgatgcat gaaagtttta gaaatcaaat tagatgttca gatgaacaaa ctgtttataa
    13981 tcttataaaa gaaaaaatag aggaggaaga ttaaaatgag aattttaaag tttttgcaaa
    14041 aacatcttat gacagcaaca tcttatatga ttccatttgt ggttgcagga ggaatattat
    14101 ttgctttatc agtaacttta agtggacaag ctgctgttcc tgaaacaggc tggttggcta
    14161 aactgaatca aattggagct gctggcttgg ctttattcat tcctatatta ggtggatata
    14221 tagcatttag tatggcagat aaaccaggat tagctccagg tatgataggt gcatatttag
    14281 caaaagaagt aaatgcaggt tttataggtg gtatcatagc aggatttata gcaggatttg
    14341 ttgttttaca attaaagaaa attaaattag ctcctacaat gagaacttta ggttctatat
    14401 ttatttatcc tttagttggt acattgatta caggtggtat aattgtattt ttaattggag
    14461 aacctatagc ttcttttatg acatggatga caaactggtt aaatggaatg tctggtgttt
    14521 ctaaaattcc tttaggtgga atcttaggag gaatgatagc ctcagatatg ggtggcccta
    14581 taaataaggt tgctgctact tttgcccaaa ctcaagttga cacacttcca tatttaatgg
    14641 gtggagttgg cgttgcaatt tgtatacctc caattggttt aggattagct acattattgt
    14701 ttccaaaaaa atttagcaag gaagaaagag attcaggtaa agcttcacta ttaatgggat
    14761 gtgttggaat tacagaagga gctattccat ttgcaacatc agatccagtt agagtaatac
    14821 cttgtattat ggctgggtcg attgtaggaa atattatggc ttttttatta ggatgtttaa
    14881 atcatgcacc ctggggaggt ctgatagtgt tacctgtagt tgacaatagg ttggggtata
    14941 ttgcatcagt attaactgga gcagtagttg ttgcggtatt aatgaaatta gtaaaaaaag
    15001 acgtgaaaga agatgaagaa attgaagaag atttagatga atcaattgaa ttaatatttg
    15061 aagaattata aatataaatg gaaggaattt gtataaaaaa gtaaatataa aactataaat
    15121 ttaaaggaga taagaatatg aaaattgtag cagtaacatc ttgtcctagt ggagtagcac
    15181 atacttacat gtcggctgaa tcacttgaat tatcagccaa aaaatttggt atagaaataa
    15241 aggttgagac tcaaggaagc tctggaatag ataatgaatt gtcacttaaa gatattgatg
    15301 aagcaacttg tgtaatattg actaatgatg ttgagattag aaatatggaa cgttttaaag
    15361 gaaagaaggt agtaagaatg agtgtatctg agataattaa gaaatctgat gcattaatta
    15421 agaaaataaa agatactttt caataaaaat tgatttgttt tacatataac tcaaagatgt
    15481 ttttaaagaa atttttatta aaagcatctt tgaaaatatc ttaaaaatag aataaaagga
    15541 gatatagtca tgaaaaagtt accaataaag actgtcgtgc aaagattact aaacttacaa
    15601 gaagagggga aaagtgcaac tcttttagga attggtccta tgtcaccaaa cttgttacaa
    15661 gcaagttttg aactagcaaa agatgatgat tttcctttaa tgtttatcgc aagtagaaat
    15721 caagtagatg cagatgaact aggtggcggt tatgtaaatg gttggaatca ggaaactttt
    15781 acaaaagata ttaagaaggt tgccgacaaa gttggatttg atggtttata ttatttatgt
    15841 agagaccatg gtgggccatg gcaaagagat aaagagagaa atgaccattt gcctgttgat
    15901 gaagcaatgg agttaggaaa aaaatcttat ttagcggaca ttgaagcagg ttttgaccta
    15961 ttgatgattg accctacaaa agacccattt gaaattggaa aagtaattcc actagatgta
    16021 gtactagaaa gaactgtaga attaattgag tattgtgaaa acgagagaaa aagacttaat
    16081 ctaccagaca taggttatga ggttggtaca gaggaaacca atggaggtct gacttcaact
    16141 gagacgtatg aaacatttat aactagacta aaggttgaac tcgataatag aggtttgcca
    16201 atgccaacat ttatagttgg tcaaactggt actttaacta gaaagacaga acaagtagga
    16261 acttttaatt ttagaaatgc ttatgattta gctcaaatgg ctaagaaata tggagttggt
    16321 ttaaaagagc ataatggcga ttacttagat gatgtaactt tattagaaca tataccatct
    16381 caaattattg ctacaaatgt agctcctcaa tatggtactg aagaaactag ggcatattta
    16441 aaattagcag aagttgagtg taaattagaa aaagagggct tagttgagaa aacttctaat
    16501 atcagacatg ttcttcttgt taatgcaatt gaatgtggac gttggagaaa atgggtagta
    16561 ggagaacaaa aaaatctaac tactgaagag atatttaagg atgaagtttt atctaatgaa
    16621 atactagata ttgcaggaca ttatacattt aataatgatg atgttaaaaa agaaatagaa
    16681 gtgctttatg ataatttaag taagaataat attgatggtc aaagatttgt tgtagaccat
    16741 attaaacgtc ctttgcgtga ttatgcagaa tgctataact taaaaggtgt tactacgagg
    16801 attttaaata aataatagga cttatagtgg tgagtattgt tatactaggt atttatagtt
    16861 ataattatac ttaccactat taaatttagg aggactaata ttttgattaa tttaattttt
    16921 gatgtagatg atactttata taatcaatta acgccttttt acactgcata taataaagtg
    16981 ttttcttcaa taaaggatat ttctattgaa gatttatata tgagtagtag aaagtatagt
    17041 gatgaggtat ttcatatgac tgaaaatgga gaaatgccaa tcaaagaaat gcatatttat
    17101 aggataatga aagcttttga ggagttggga aattctatta cagaaaaaga tgcacaaagt
    17161 tttcaagatg agtatatata tcaacaatca caaattacac taataccaga agttgagagg
    17221 attttaaatt tttctaaaga aagaaatatt aatctaggaa ttataactaa tggcccatca
    17281 aaccatcaaa gaatgaagtt aaagcagtta aatattgaaa actgggttga taaaagtaat
    17341 atttttattt ctagtgaggt tggattttct aagccagata ctaatatttt tagagtagct
    17401 gagaatgtta tgaatttaga tagagaaaat acatattatg tgggtgattc ttatagaaat
    17461 gatgtattag gtgctaaaaa agctggttgg aagagtatat ggttgaacca tagaggacat
    17521 gaggtagaag agttgtttta caaacctgat tttgtaattt tggaacataa ggatttgata
    17581 tctttattta taaaaatatg tagttataaa aatatgtagt aaaaaaatgt taataataga
    17641 attgtataaa ataattaata ataactatat aaaattatat gtttagattg tgagcaatct
    17701 attttatgtt aaaaatagat tatttagaag aaggggaaat cttaaaaaaa tttgaatatc
    17761 ccctttttat tatttaatta aaatgaaata gttagaaata tattttattt agtttagtca
    17821 ataacatttt gggattcttt ataactacat gttaactgtt gaataggttt atcatatggt
    17881 ttaacccatc ctttattaat acttaattct gtaagtgctg tgtgaccttc taccatttga
    17941 gttaatgaag ctgaatatat agctcttaat tcaggtgtac tacttgtcaa tgcagaatta
    18001 agatacatat tggcagatgc tttagcagat gaaagcatac ttaacattat tactttatcg
    18061 tctatataag tattgttctt aattagattt tctatcatat ttctcatgtt atctatacct
    18121 cctcagaaat tagtatttca ttttcaacta taaattgttg catagcttta attcttcctt
    18181 ctgttgctaa tatactagat tctgattgtc tttttaggtc ttcatctgat attaacatat
    18241 ttattgctct ttgcataagt aatccgtcag actccatttt tataactgct gctaaagata
    18301 ttttttcagt ttctgaaagt attctcattt aatcatctcc tatattgtaa tttatgcttt
    18361 aatagtatgt ctttcttttc aaggaaataa ccattacact tttttacagt attttatatt
    18421 ctctttatat caaaatttag tcgacctaat atattaataa taattttgta ggatatttat
    18481 cattaaatat ctataaaata aattatttga atattcataa agcaaaattt atttgtatgt
    18541 acttcttcaa gagtcaatat gagttttttc aaaaaattaa tttttatgat aatcattaag
    18601 ggctatattt ttcttaaagt tgtgattatc attattaatt tacaataaaa ttgccgaaaa
    18661 gccctctcct ttaggtgtag ggatgaaagg taaggtctta atataaaaaa agttagaata
    18721 atgcttgtaa tctaataccg ctactgatac cctatattta ggaggtgtta gtttttatgt
    18781 ataaatctaa caaaaatata gtttatgatt gcaaatatca tattgtattt tgccctaagt
    18841 atcgtaaacc tgttttagtt ggcgaagttg aaaagatgtt aaaagaaata ctaccatata
    18901 aagcagatga acttggagca gaaataatag aaatggaaac tgataaagaa catgtgcact
    18961 tattaatatc ttgtgaccct caatatggaa ttcataaagt agtaaaaggt ttaaaaggct
    19021 ttagttcgag agtattaaga gagaactttc cacatttaaa gtctagtatg cctagcatgt
    19081 ggactaatag ctattttgta ggtactgttg gttctgtgag tttagaagta gttaaacaat
    19141 atatagaaaa tcaacaagtt aggacaaaga aatgataaaa actcatagag tgaaattaaa
    19201 cctaactaga acacaatttg aattagtaag ggaaaagcaa atggaaagtg ctaactgttg
    19261 gaactacata gttaacttat ctaaagaata ctattttgaa cataaacaat ggattaaaaa
    19321 gaatgatata caaaagttaa tcaaaggtaa gtacaatctt catagtcaaa ctattcaagc
    19381 tatatcagat aaatttgatg caaatagaaa gactatatca gaattaagaa aaaaaggtaa
    19441 tactaaagca aaatatccat acaagactaa gaaattttat ataatacctt ttaaagctag
    19501 tgctattaat agaaatgcta aaggcaactt aaaattatca atgtcaaaag gcaggtattt
    19561 agaattagat tttaatgttg agaatataaa aactgctgaa atagtatggc gaaatggata
    19621 ttatttatac tacacttttg acaatgaatt aaatggagtt gtagccaaag gcactaatac
    19681 cgtaggtgta gacttagggg aaatacattc gatagctagt gtaacaaatg aaggtgtagg
    19741 gcttattcta tctaatagag aaggcagaag tattaaacaa tttagaaata agatgtatgc
    19801 ttatatatct aaaagattaa gtaaatgtaa aaaaggtagt agacaatcaa agaagttatg
    19861 gagattaaaa aataagataa gaagtaaaac agataatcag ttaatgaatt tatatcatca
    19921 aacaactaga aagtttatag acttttgtgt agagcagaag gtatgtgaaa tagttcttgg
    19981 ggatataaaa ggggttgaaa aagatactaa gaaaaagaaa agattaaaca gagttaatag
    20041 acagaaaata tctcaaatgg agtatggtcg aataaaggac tatataaaat ataaagcaaa
    20101 agagcaaggt attgaagtta agttagttaa agaaaactac acttctcaaa cttgcccaaa
    20161 gtgttctaaa aaacacaaac caactggtag aacttatagt tgtacttgtg gttatgaaac
    20221 tcatagagat atagttggtg cttggaatat tttaaataaa aagcataaat atggtttagt
    20281 agattttaga ataaatcata agcaaccaat aaatttaaaa gtatctaccg tttgagccat
    20341 gtgtgggcga ataactttaa ggtagtagtg gcgaagacac cgtccgaagt agcttggctt
    20401 tgtaaccacc tctgttgtac tgtttaagta taaataagtg tgttagccac caagaatccc
    20461 cttgctttag ctatggggag tgtgtcaata aaatacttta atgaaaaata gctaacttta
    20521 attgtaggat attcttaata tatttgaaat aaataaaaat attgaaggat ataatattca
    20581 attggtatat aaataataaa tgatagttaa attttacctt agaggctaat taggtgttta
    20641 gtcaaagcta atgcctttag aatacaaatg tattctaaag gcatttttac tatgtatttt
    20701 catttatatt actaaacatt atctattttt atatacattt ctatgtattt tattttcatc
    20761 aaatcggtct ataaactcat ttttttcttc actatatcca atagatataa gtgcataagg
    20821 aactaatttt aatggtaagt ctaaagtttg tctaacttta tttaccactt tatttttagg
    20881 aaatactcct gcccaacaag cagctagacc ttgattagta acttctaata ataaattttg
    20941 tgtgcaagca cctaaatctt gtatccattt accaggtttt aagaatttat ctctatttcc
    21001 aagcactact atacataatg gtgcaaattc tatatgcttt gctctatgct gtgactttga
    21061 gagctgtttc agcagttcct tatcatctac tataataaat tcccaaggtt gagcattttt
    21121 tgatgaaggt gcttgcatag ctgattttag tatttcagtt attttatctt cttctatatt
    21181 tttatctata tatgttctta ttgaacgtct attttttata acttctaaca tacagattct
    21241 cctttaaata aaattatttc tatttatata atatcattac tttaaatttt agctattact
    21301 tatataaaga ataaactttg tgttggtttt atctaagtaa aagtaacatt cagaactaaa
    21361 aaacaagact ataaatccaa agtattaatt aaaaaagaag taataaaaaa taatatacaa
    21421 gatttaaaaa agagtattgg gcttaaaatt taattagaaa accaattttg agataatctg
    21481 ttttttattt taacaaaaaa acacaaataa agttcgatac gtagcgaatg aaaaaattac
    21541 aagaaatatt ttttttaaaa aatttcaaaa aagagttgat aatatatgta tattaatata
    21601 aaatttaagg tgtataattc gtataataat acgaatgatt tgtagtgtaa aataacttat
    21661 aaacggagag gggaagtcta atgaaagtag cagtagttat gggttcaaaa tcagattatc
    21721 caaaattaga agaagggata aaactgcttg aaaaatatgg aatagaagtt gtagcaagag
    21781 cattatcagc tcatagaaca ccagaacaac tttcaatatt cctaaaagaa atagaagatg
    21841 atactgatgt aataatagca gcagcaggaa aagcagcaca cttaccaggt gtaatagctt
    21901 cacaaacatt aattcctgta ataggattac ctattaaatc atcaactatg gatggactag
    21961 actcactttt atcaatagtt caaatgccaa aaggaatccc agttgcaaca gttacaatag
    22021 acttaggttt aaacgcagct ttattggcat tacaaataat gactttaaaa tacccaaaat
    22081 taaaagagga tttgaaatca tatagagaag agatggcaca aaaggtgtta gaagacgaca
    22141 agaatttaag ggggtagcag aaatgttatt atatgaagga aaagcaaaac aagtatattc
    22201 tacagataat gaaaatgaat atgttgttta ttataaagat gatgcaacag catttaatgg
    22261 agaaaaaaaa gcagaaatat cttcaaaagg tatactaaat aataagatat caacaataat
    22321 ctttgaaatg ttaaaagaaa ataatataaa tactcacttt ataaaaagtt tatcagatag
    22381 agaaatgtta gttaaaaaag tagagatttt accattagaa gtaatagtaa gaaatattgc
    22441 tgctggttct atctgtaaaa gagttggtct tgaagaaggt gtagttttcg atgagccaat
    22501 ttttgaaata agctacaaaa atgatgctta tggtgaccca atgctaaatg atgattatgc
    22561 agtggcaatg aagttagcaa ctagagaaga gttaaaattc ttaagagaag aaactttaaa
    22621 aataaatgag ttactaaaag catttttctt aaaattaaac ctaaagttag ttgactttaa
    22681 aatagaattt ggtaaggatt ctgaaggaaa tataatattg gcagatgaag tatcaccaga
    22741 cacttgtaga ctttgggatg taaatacaaa tgaaaagtta gataaggaca gatttagaaa
    22801 agaccttgga gaccttgtag aaggttatac ggaagtactt tcaagaatga ataataaata
    22861 ggaggcaaat gtatgtgtgg agttttaggg atttactcta ataaagatgt aactaaagaa
    22921 ctatattatt ctttatattc tatgcaacac agggggcaag aaagctgtgg tttagctctt
    22981 ttagatgatg gagaaattaa gtacaaaaaa gatatgggat tagttggaga tgtttttaag
    23041 gaaaatgaat tatctaagtt aaaaggaaat atagggattg gacatgttag atattcaaca
    23101 gctggaggaa gtcatgtttc taactgtcaa cctttagttg gaagttgtag aaaaagacaa
    23161 ttagccatag ctcataatgg aaatctagtt aatgcaaatt atttaaaaga tatgctagaa
    23221 gaagatggat atatgttcca aactaattca gatacagaag tgattttata tatacttgct
    23281 agatattaca aaggggatat agtagaaagt ttaaaagtga caatggatta tataaaaggt
    23341 gcatatgctc ttgtaataat gagtcaagaa gaattggttg cagttagaga cccacatgga
    23401 ttcagacctc ttgtacttgg taaaaagggt gatgaatata tatttgcatc agaaaattgt
    23461 gcaatagata tacttggcgg agaagttata agagatgtag agccaggaga gataatagta
    23521 gtcaaagatg gagaactaaa atcatatttt tattcagaaa attataaacc agttaagaaa
    23581 agttgtatat ttgaacacat atactttgca agaaatgatg caactataga caatgtaaat
    23641 gcatatgaat ttagaattaa atgtggtgag agattagctc agaatgaaac tgtaaaggca
    23701 gatatggtag ttccagttcc agattcaggt tggccaggag ctattggtta tgcaaatgct
    23761 agtggattaa aaataagtga aggcttagtt aaaaatagat atgtaggaag aacatttata
    23821 aaacctacac aagaagaaag agaaatagca gtaaaaataa aattaaatcc tctttcaaca
    23881 ataataaaag gaaaatctat aattttagta gacgactcaa ttgtaagagg aactacatct
    23941 aaacaattag taaaatcttt aagagaagca ggagcaaaag aaattcatct tagaataact
    24001 tctcctccag tagcttactc ttgttactat ggaatagata ctccaaatcg ctctaaatta
    24061 attgcatcaa gtaacaacgt agaagaaatg agagaatata ttggatgtga tagtttaaaa
    24121 ttcctagata tagaaggaat gttagatgca acagaacata agtctacttt ctgtaaagct
    24181 tgttttgatg gagaataccc agttaagaaa attgataagg aggaactact ttcatgttaa
    24241 catataaaga atcaggtgta gatatagatg aaggaaatag agcagtagac cttataaaag
    24301 gtaaaataaa aggaacttat gatggaaatg tagttggaga tttaggtaac ttcagtggat
    24361 tatacagttt aaaagatttt gtaggtatga aagaaccagt tttgcttgca tctacagatg
    24421 gtgttgggac taagttaaaa atagctcaga tgatggacaa gcatgatact gttggaatag
    24481 atttagttgc aatgtgcgtg aatgatttaa tttgtcaagg agcaaaacca ttattcttcc
    24541 ttgactatat agctcttggg aaattagtgc ctgagcatat cgaaaaaata gttggtggaa
    24601 ttgcagatgg ttgtaaaatg tctggttgtg cactaattgg tggagaaact gctgaaatgc
    24661 ctggaatgta tggagaagat gactatgatt tagcaggatt ttctgtagga atagcagaca
    24721 aagaaaagat tgtttctgga aacaatgtta aatcaggaga tgtattggtt ggtatatctt
    24781 caagtggagt acacagcaat ggattttctt ttataagaaa gatattttta gaaacttaca
    24841 attataaaat ggaacaatat gttgaagaac taggaatgac agttggagaa gcacttttaa
    24901 caccaactaa aatatatgtt aaattagctt tagatgtatt agctaaacat gacataaaag
    24961 ctatagctca cataactggt ggagggctta tagaaaatat aacaagagtt atacctaaag
    25021 ggttaggact agatatcaat aaaaaatctt gggagaagcc tcctatattt aaaatgatag
    25081 aaggttttaa tgctgttgat gagagagaac ttcataagag ttttaacatg ggtattgggc
    25141 tagttttaat agtagataaa gaaaatgctg atgatgttgt aaactttata aataatagag
    25201 aaaatgacaa tgctgcttat gtagataaaa aatatagtga attattagaa gataaagctt
    25261 atataattgg tgaagtagta gatagtcatg aaggtgtaga attatgctaa atataggagt
    25321 tttaatatct ggtggaggaa caaatttaca agcagttata gatggtactg agtctggaga
    25381 aataaaaggt caggttaaag tagttatctc aagtaagcaa ggtgcttatg ggctagagag
    25441 agctaaaaat cataacataa aagcaatttg tgaaactgat gaagacaaga taatagaaat
    25501 tcttaaggaa aataaaatag atttagtagt tttggctggt tatttaaaaa taatcagccc
    25561 aaagctggtt aacgagttta gaaacaaaat gataaacata catccatcat taattccttc
    25621 attttgtgga gctggattct atggagaaaa agttcatcaa ggtgttatag attatggagc
    25681 taaagtaact ggtgctacag ttcattttgt agatgaaggt gctgatactg gaccaatcat
    25741 aatgcaagat gtagttaagg taaatcaaga tgatgatgca aagacacttg caaaaagagt
    25801 gttagaagtt gaacatagga ttttaaaaga aagtataagt cttttttgtg aaaataaatt
    25861 aaaacttcaa ggtaggagag tgtttataaa tgagtaagag agctttaata agtgtaactg
    25921 acaagactgg tgtagtggaa tttgctaaag agttaaataa attagattat gaaattatat
    25981 ctacaggaaa tacatttaaa acattaaaag aaaatggtgt taatgtaatg caagttgaag
    26041 atgttacaaa ttttccagaa atattagatg gaagagtaaa aacattaaat ccatatatac
    26101 atggtggtat actttataaa agagataaag aatctcatgt agaaactgta aatgaacaca
    26161 aaatacattc aatagattta gtagctgtaa atctatatga ttttgaagga actttaaaag
    26221 ctggaaaaag tcatgatgaa ataattgaaa atatagatat tggtggacca tctatgatac
    26281 gttctgctgc caaaaactat aaggatgtaa tagtagtagt agatataaaa gattatgatt
    26341 ctataataga aaaattaaaa acagatacaa tgacattaga agacagaaaa aaattatcat
    26401 ataaagcttt ctcaactaca ggaagatatg atgcgttaat atctagttat tttgctggag
    26461 aagttggaga tacgtatcct gacatattaa atttaacttt ccaaaaagaa caaactttaa
    26521 gatatggaga aaatccacat caaaatggtt tcttatattc acagtcaaat gcaaaaaatc
    26581 caatcttaaa ttatgagcaa ttaggcggta aagaactttc ttttaacaac ttaaatgatt
    26641 tacatggatg tttagaagta atgagagaat ttaaagatag tgaagaagta gtttcagtag
    26701 ctataaaaca tgctaattca tgtggggtag gtttaggaaa agatgctttt gaagcatata
    26761 caaaatgtta tgaagcggat aaggtttcaa tatttggagg aatagtagga ataacttcta
    26821 ctatagataa agctacagca gagaaattaa atgaaatatt cttagaaata gtagtagcat
    26881 atgattttga accagaagct ttagagatac ttaaacaaaa gaaaaatcta agaatattaa
    26941 aattagctaa aatagaaaat agtttacaac catatgaaat gaaatattta gatggaaaat
    27001 tattgataca agatagaaac aatatattag cagaaaaaag tgaaaatgta acaaaggaaa
    27061 aaccaacaga tgcacaactt aaggacatgg aatttggtat gagagtagta aaaaatatga
    27121 aatcaaatgc aattgcagta gtaaaaaatg gacaaactct agctttaggt tgtggacaaa
    27181 catcaagaat ttgggcatta aaaaatgctc ttgaaaataa caaagataaa gattttacag
    27241 gagctgtttt ggcgtcagac gcatttttcc catttgatga ttgtgtaaca ttagctcatg
    27301 aatatggaat atcagcagta gttcaaccag gtggctctat aaaagataaa gattcaatag
    27361 aagcttgtga caagtatgat atggttatgg tatttacagg aataagacac ttcaaacact
    27421 aaggaggata ttatgaagat attggttgta ggcggtggcg gaagagagca tgctatatgc
    27481 tggaaactaa gcaaagaaaa aaatgtagaa aaaatttatt gtgcaccagg taatgcagga
    27541 attgcaaacg tagcagaatg tgtaaatata ggtgatacta atatagaaga acttttaaaa
    27601 tttgccaaag aaaatgaaat aggtctgaca atagtaggac cagaagtacc actagttatg
    27661 ggaatagtag atgagtttga aaaagaagga ttaagagtat ttggacctaa taaaaaatgt
    27721 gctcaacttg aaggaagtaa agctttttca aaagaattta tgataaaaca taacatacct
    27781 actgcaaaat ataaagaata tacaaactta gaagaagcta tatcagaaat agatagtttt
    27841 ggatatccag tagtaataaa agcggacgga ttagctgctg gaaaaggtgt tgtaatacct
    27901 gaaaatagag aagatgctat agcaacttta aaagaaatga tgtcagataa gaagtttgga
    27961 gctgctggag ataaaatagt aattgaagag tttttaaaag gaatagaaac ttcaattcta
    28021 gcatttgtag acaatgatac aatagttcct atggcaagtg caaaagacca taaaaaagta
    28081 aacaactatg agcaaggtcc aaacactgga ggaatgggaa ctttttcacc tagtgaaata
    28141 tatacagaag aattagcaaa taaagtaaaa gagacagtat tagaaaaaac tttagaaggt
    28201 ttcaaaaaag atggacttaa ttttaaaggt attttatttg ttggtcttat gataactgaa
    28261 gatggagaaa aagtacttga atataatgta agatttggag accctgagac acaatctgtt
    28321 ttatttagat tagaaacaga tttacatgag ataatggaag ctatactaga taataagtta
    28381 aaagatatag aaataaacta tagtgatgat gaagctgtat gtgttatgct tacatcagga
    28441 gggtatccag acagttatga aaaagggaaa attataactg gtcttgaaaa cttggatgat
    28501 gatatagtag tattccatag tggcactaag atgtttgatg gtaatttggt taccaatgga
    28561 ggaagagtaa ttggtataac tgcaaagtca actagagtta aggatgcagc agaaaaagtt
    28621 tatgaaaaca taaagaagat aaactttgaa ggaatgcact atagaacaga cataggaaga
    28681 taatttttct aaaatggagg ataaggtatg ttgaatactg aaaataaaga ctcaatggtt
    28741 agaagagtat tggttgaaaa aagagaaggt tttgaccttg aggctaaagc tttaaaaaaa
    28801 gacttagtag aaagtttaca catagataat atagaaaatt taagaatatt aaatagatat
    28861 gatgtagaag gaatatcaga agaagtttat gaaaatgcag caaagacaat attctctgaa
    28921 ccaaatttag atgtagtata ttatgaagaa ataccaaaat taaatgatga aagagtattt
    28981 gcaatagaat ttcttcctgg tcaatacgac caaagaggtg attgggcagc tcaatgtgta
    29041 caaattgtaa atcaagggat aagacctgct attaatacag caaaagttta tatattatca
    29101 ggcaaaataa ctgatgaaga gttttctaaa ataaaagatt actgtataaa tccagtagat
    29161 agtagagaag catctttaga gaaaccagaa acgttaaaaa tggaaactga aataccaaca
    29221 acagttgaag tattagatgg atttatagat ttagatgaaa atggattaag aacatttgta
    29281 agtgaaaaag gattagcaat gactttagga gacttacaac acgttcaaaa atactttaaa
    29341 gatacagaaa agagaaatcc aactataaca gaaataaaag tattagatac ttattggtct
    29401 gatcattgta gacatactac ttttatgact gaaatagaaa atgtaaagat agaagatggt
    29461 aagtttaatg atatagtaaa agaagcgtat caaatgtatc ttaactcaag agacaatgta
    29521 tatgtgaaca gacataaaga tatatgttta atggatatag ccactgttgc agtaaaagaa
    29581 ttaaagaaaa atgggaaatt aaatgaccta gatgaaagtg aagaaataaa tgcatgtagt
    29641 ataaacgtag atgttgaagt tgatggtaag atggaaaaat atctagtaat gtttaaaaat
    29701 gaaactcaca accatccaac tgaaatagaa ccatttggtg gagcagctac ttgtttagga
    29761 ggagctataa gagacccatt atcaggaaga agttatgttt atcaagcaat gcgtgttaca
    29821 ggaagtgctg acccaagaac gactttagaa gatactttac ctggaaaact tatgcaaaga
    29881 aaaataacta cagaagctgc tcatggatat agttcatatg gtaaccaaat aggtcttaca
    29941 actggacaag ttgcagaagt atatgatgaa aactttgttg caaagagaat ggaaataggt
    30001 gcagttatag cagcagcacc aaaagagaat gtagtaagag aaagacctga agcaggagat
    30061 gtaatcgttc ttcttggagg aaaaacagga agagatggct gtggaggagc aactggttca
    30121 tctaaagaac atagtgaaga gtcaatctta acttgtagtg cagaagttca aaaaggagat
    30181 gctcctaacg aaagaaaaat acaaagattc tttagaaata aagaagttgc tcaaatgata
    30241 aaaagatgta atgattttgg agcaggtgga gtttgtgttg ctataggaga gattgctgat
    30301 agcttagata taaacttaga cttagttcct aaaaaatatg atggacttga tggaactgag
    30361 cttgcaattt cagagtcaca agagcgtatg gcagtagcca taaaaaaaga aaataaagat
    30421 aaatttatac aattagcagt agaagaaaac ttagaagcta cgcatgtagc tacagtaaca
    30481 gatacaggat atttaagaat gttctggaat ggaaaagcaa ttgtagatat aaatagagag
    30541 tttttagata caaacggagt aaaacaaact actgatgttc atgttactaa agtagatgaa
    30601 gaaaatacat tctttagctc taatgaaata gtaaaagatg ttaagtgtgc aagtatgaaa
    30661 gacaagttta caaaagtact atctgacctt aatgtttgtt ctcaaaaagg attagtagag
    30721 atgtttgata atacaattgg tggtaataca gtacttatgc catttggagg aaagtatcaa
    30781 gcgactccaa cacaaggtat ggttgctaag ataccagtac ttggaggaga aactaatact
    30841 tctacaataa tgacttatgg atacaatcca aaggtaggaa aatggagccc attccatggt
    30901 gccttgtatg cagtagtaga atctgtgtgt aaattggttg ctattggagg aaattatagt
    30961 acaacaagac ttacattcca agagtatttt gaaaaacttg gtaacaatcc agaaaaatgg
    31021 ggtaaaccat tctcagcact tttaggagcg ttttatgcac aaagtaaatt tgagatacct
    31081 gctataggtg gaaaagacag tatgtctggt acatttaaag atattgaagt tccaccaaca
    31141 ttagtttcat ttgctgttga cacagtagat gcgaagaaag tagtatctcc agaatttaaa
    31201 aaagcagact caaaagtagt aatgttatgt gtaaacaagg cagaaaatga tgttgtagat
    31261 tttgaggaat taaagagaaa cttagataaa gtaagagaac ttatacatgg aaataaagtt
    31321 ttatcaacat atgcattagg atttgcaggt gtaggtgaag caataagtaa aatggctttt
    31381 ggtaataaaa taggatttaa gtttagtgaa gaagctgaaa aagcatttac agataataaa
    31441 ttatttgagg ctagttatgg aaatatagta cttgaattag caaatgatga tttaagtatg
    31501 ttagaaggat acaattatgt tgtgattgga agtacagtta aagaagctag tatatttatc
    31561 aaaggtgaag aacttgcatt agatgaatta tataaagcac attgtagtac tttagagcct
    31621 atattcccaa ctaaaacaga agaagtaaaa tcaaagatag aaactataag ttatatatca
    31681 caaggagaag ctaaaaaatc atcattaagt atagctactc caagagtatt tataccagca
    31741 ttccctggaa caaactgtga atatgactct gcaagagcat ttgaaagagc aggagctaat
    31801 gcaagtataa gagtatttaa gaatttaact tataaagaca tagaagattc tatagataca
    31861 atagtaaacg aaataaaatc ttctcaaata ataatgttac caggtggatt tagtgctggt
    31921 gatgagccag atggttctgg taaatttata gctactgtat ttagaaatcc tagagtgcaa
    31981 gaagctataa atgaattttt aactcaaaaa gatggactaa tgttaggtat ctgtaatgga
    32041 ttccaagtat taattaaatt agggttagtt ccatatggag aaataagagt tccaagtgaa
    32101 agtgctccaa cacttactta caataatatt ggtcgccatc aagctaagat tgctagaact
    32161 agaatttcat ctaataaatc tccttggtta gctcaaacta atgttggaga tattcataat
    32221 atagcaattt ctcatggaga aggtaagttt gttgccagtg aagatgttat gagagaatta
    32281 attgctaatg gacaagtagc aactcaatat gttgacttta ataatgaagc tacttatgat
    32341 attgagttta atccaaatgg ttcattctat gctgttgaag gtataactag tgctgatggt
    32401 agagtgtttg gtaaaatggg acattctgaa agaattggtg aggaagtata taagaatatt
    32461 ataggtgaaa aagaccaaaa gatatttgaa tctggagtta aatattttag ataaagcttt
    32521 gatgtttaat tgatagctag aatatataat ttagatattt agatttaaag agatgtaata
    32581 atatgatgat aatcatttaa tattacatct cttttttatt tatctaagaa ataataagtt
    32641 caacctaagt taaaatacaa gctgtgttat aattaattat taagtaaaat tgaatattta
    32701 ttggagggca acataattaa tgaaaatatt aataacaggg tctaatggtc aattaggaaa
    32761 agaattggtt aaccaattag aagcaatcaa tcaaagtata aaccaaccaa aatatgttat
    32821 attagctaca acaagaagtg atttggacat atccaatcag actaatgtag ataattttat
    32881 tttgcataat aaacctgatg tagttgtaaa ctgtgcagca tatacaaagg ttgatgcatg
    32941 tgaagataat atcgaaattg catacaaaat aaatgcttta ggagtgagaa atctagcaat
    33001 agcatcagaa aaagttaatg ctaagcttat tcacatatca actgactatg tattcaatgg
    33061 tttttctaaa tatccatata gggaagacaa taaaactgaa cccaattctg tatatggaaa
    33121 aagtaaactt atgggagaaa aatttgttga acaattctca cataagtact ttatattgag
    33181 aacagcatgg ttatatggta atgggaataa ttttgtaaag accatgataa aactttcttt
    33241 agaaaataaa gaggttaatg tggttaatga ccagtttgga actccaacaa gtacagtaga
    33301 cttagcaaaa attataataa gaatcatgga aacagaacat tatggagttt atcatgctac
    33361 atgtgaaggt gagtgtagtt ggtatgattt tgccaaaaag atatttaaac taaaaaatat
    33421 agatataaaa gtaaatccaa ttaagagtaa cgaatttaaa tgtaaggctc caagaccact
    33481 atattctgta ttggataatt ttatgttgaa gttaattggt ttaaattctt ttagaaagtg
    33541 ggaagaatcc attgaagaat atttagaaag atatgatata aatgaaaagt aaatgtatta
    33601 ttttgagaaa agaaaataag atattataaa aaatataaat atttctttta tttatccgat
    33661 aatcataata tgacaaaaac aaatttaagg atggaaaaag atgaaaggaa ttattttagc
    33721 aggagggtct ggtacaagac tatatcctat aacaaaatgt atatcaaaac aaacacttcc
    33781 aatatacgat aaaccaatga tatattaccc tatgtcggtt cttatgttgg cagggataca
    33841 agatatatta ataatatcta caccaagaga tataaaagca tttgaagaat tatttggaga
    33901 tggaagttca ctagggctta acataacata tgcaattcag gagaaagcaa atggaattgc
    33961 agaagcgttt ataataggag aagaatttat aggaaatgac agagtgtctc ttgtattagg
    34021 ggataatata ttttatggat atggatttag tgaaaggttg gagaatgcag ttaaaagaaa
    34081 aggagcaact atatttgggt atcatgtaag taatccaaat tcgtttggag ttgtagaatt
    34141 tgataaggat tttaatgtat tgtcaataga agaaaaacct acagttccta aatctaatta
    34201 tgctatacca ggattatatt tttatgataa tgatgttgta gagatagcaa agctagttga
    34261 accgtcagat agaggtgaat tagaaataac ctctataaac agtgaatatc taaaacgagg
    34321 aaaactaaaa gtggaacttc taggaagagg tatggcttgg cttgatactg gtacacataa
    34381 aggtctattg gatgcttcta attatgttga agtggttcaa acaagacagg gactgtatat
    34441 agcatgttta gaagagattg catatacaaa aggatatata agtagagaac aattaatgaa
    34501 actagccaag ccactgataa aaactgaata tggtcaatat ctattaaaat tggctagtga
    34561 agatatgtaa tatggtagat aaaatttatt ataaatttta ttaagggagt ttattataat
    34621 gggaaaaatc aatgttataa agactaaaat agaaggatta tatatagtcg aaccaaatgt
    34681 attttatgat gaaagaggat attttatgga aagctataat aaaaaggatt ttatagagca
    34741 gggtcttgat atgtgtttta ttcaagataa tgagtctagc tcaagaaaag gcgttttaag
    34801 aggaatgcat tttcaggtta aatatcctca aggaaaatta gtcagagtta cagagggaga
    34861 agttttcgat gttgcagttg atttaaggaa agattcacaa acatttggga aatgggaagg
    34921 tgtaatattg tctgataaga ataaaaaaca attttatatt ccagaagggt ttgcacatgg
    34981 atttttagtt ttatcagaaa aagcagtatt tagttacaaa tgtacaaatt attattaccc
    35041 agaatttgag aatggcttta tatggaatga caagcaaata tctattgagt ggcctttaaa
    35101 aggaattgaa gaaatatttt tatcagaaaa ggacaaaaaa caaaaaagtt ttaaagatat
    35161 ttgtacaaga cgttaataaa aatttattag aattaacatt taggagaaaa aatatgaaaa
    35221 aaatactgat aactggagga gcaggattta ttgggagtaa ttttatacat tatatgctta
    35281 acaagtatgt agattatttt attgttaacc tagacttgtt gacttatgct ggtaatttag
    35341 agacattaat agaaatacaa gacaattcca attataaatt tgttaaagga gatatttcaa
    35401 atagggagca ggtgtataag ctatttgaag aagaaaggtt tgatatagtt gttaactttg
    35461 ccgctgaatc tcatgtagat aggtcagttg agaatcctga tttatttatt aaaacaaaca
    35521 ttataggaac acaagtactt ttagatgcat ctataaaata tggagttaaa aggtatcatc
    35581 aaatatctac agacgaagtg tatggtgatt taccaattga tagaaaggat ttattcttta
    35641 cagaacaatc ttctatcaat ccaagttctc catattcagc ttctaaagca tcagcagatt
    35701 tattggtaag ttcttattat agaacatatg gattgttaac aactatatct aggtgttcca
    35761 acaattatgg gccatatcat tttcctgaaa aattgattcc tcttatgatt ttaaatgctt
    35821 tggaaaacaa acaattgcca gtatatggga atggggaaaa tgtaagagat tggttacatg
    35881 tatatgacca ttgtactgct atagacttaa taatacataa aggaaatata ggggagattt
    35941 acaatatagg aggtcataat gaaagaagta atctagaagt agtaaaaatg atattaaatc
    36001 ttttaggtaa atctgaagag ttaatatcct atgtaaatga tagacctggt catgatttaa
    36061 gatatgctat tgatgcaact aaaatagaga atgagttagg ttggaaggca aaatatgact
    36121 ttgatttggg tataaaagaa actgttaagt ggtatataga aaatgaatct tggtggaaag
    36181 ctgttttatc agtataactt taaaatttag aaaggggcgt tttatattga atgagataag
    36241 ttcactaatt aatatattag cattgactca attatctggt agtaatacta caaatcaatg
    36301 taattgtaca tataataatt catctggatt tgatatgatt atgatgacct tgttaaaagc
    36361 attatcacaa aataatcaac atacttctaa tagctacaat ttattgtcta attctgagaa
    36421 tttatttaat gaacttgata atgtagtaaa taataccact agtagattta tagatgttga
    36481 tacaaaagat aaaaatgtaa agttgagaat tgaaaatgct gtagatcaag catcaaaaaa
    36541 atataacgta gattcaaatc ttataaaagc aataattaaa gttgaatcag attttaatcc
    36601 aaataccgtt tcttctgctg gtgctaaggg tcttatgcaa cttatgccag aaaactgcag
    36661 agacttagga gttacagacc cttttaatat agaacagaat atagatgctg gaactagaca
    36721 tattaaggaa tacattgata tgtttggagg gagcatagaa atgggattga tggcatataa
    36781 tggtggtcct ggaagaatga aaagtagagg agtagaatca atctctgacc tgtataaaat
    36841 gccaaaagaa acacaaaatt atattcctaa ggttatgaaa tattatagag ggtaggcttt
    36901 attatgggaa taaatataga taagctaaac ataagtccaa gtataaatat ggaggttaaa
    36961 aaactaaaag agagtaataa attttctgac aaattaaact ctttaaaaca atcttcttca
    37021 ttggaagata taaaacgaaa tttggaaagt gtaaaaaagg caggagaaag gcttgtactt
    37081 actcaaaact atggcgatat tagtatatat aaaaatgcag taaaagaata cctaaaatct
    37141 gttgtagata atatgtattc acttgataaa aatagtagct tttgggagca tcagtattat
    37201 aagaatgtaa agattgttga tagtaaactt gaggatatga ctagtaagtt aattagtgaa
    37261 gaaaaagaaa acatagatat tgtttctact gttgacatga tacaaggctt attgatagat
    37321 atgtatagat aaggttatac aaggagaaaa attttatgaa tagcaaaaaa gaggatatat
    37381 atgaattgaa ttttgggcaa ttaatagaag aaaaatctgg tgttatagaa gaaaatcaaa
    37441 atgtttttaa tgcaaaagta aatgtagctg tttctatagg aaatacaaca gaaacaataa
    37501 aaaatatatt agatttaaaa gaaggctcta taataaaatt aagtaagaat gtggatgaaa
    37561 aattagatat atatgcaaac gatagattgt ttgcttatgg agaaagcatt attacaaatg
    37621 agaggttatc tgtaagatta tctaaaatag aagatccaga tgtaaattag cattttaaat
    37681 agtatccctt taggctattc tgtatgagtt tagctaaggg gatattttta taaaaaattt
    37741 aaaatatgtg tgaattatat aaatattttt attttatgag cgataactaa attataagta
    37801 tgtaattttg gaggtgaaac tatggatatt aaaaatgtaa gttcaaatat tgtagtgaat
    37861 aattatttaa agaatacatc tggtataaaa aaagtggaag aggttaaaaa aattaataaa
    37921 acagataaga tagagttatc taaatttgca aaggaaatgg caagtgtgtc aaatatgagt
    37981 gatgagaata ttagaaataa aaaagtcgat gaaataaaaa atgcattaga aaatggaact
    38041 tacaaaataa aaccaaaaga aattgcgaaa aaaatgatag aggatatgcg aggataaagt
    38101 catgattaca gacttaaaag ttataatata tgaagaaaaa aatataatta gtaagatgat
    38161 ggaattatta aaagaacaat ttgattatat agttgaaaaa gacttagaaa atttaaatat
    38221 gttaaatcct aaactagaag atattagcag ggagcttgca tccatagaaa taaagagaag
    38281 acaacttttt ggtgatgata tttcaatgag tgaagttgtt gaaaatagta atgatggata
    38341 cttaaaagaa atatatacag acttgaaaca cattcttgct ttagctaaaa atcaacaaga
    38401 atcaaatgat tctctaataa agaaagaact tatttttacg aaaaaaatga ttaatttcat
    38461 aaaaccagct gataagcaaa ctacaactta taattcttat ggaaatatta gaaaataaat
    38521 attaggagga gaaatatggc aggattattt tccattttaa atacagctaa ttcaggaatg
    38581 aatgcgcacc aaaaagcaat acagacaatt tctcataata taagtaatct agatacagac
    38641 ggttattcta ggcaaaggac cgagtttgcc acaaatagtc caatgtatat gcctagtttg
    38701 agtaattcaa ttgcaagagg acaattaggg actggagtac atgtaacgga tgtaacaaga
    38761 gctagaaata gtttttatga ttaccaattt agggcagaag ctcataagta tggtaagaca
    38821 gtttcaaaat atgattatta caatactata gaaactatac taaatgaacc atcagactat
    38881 ggaatatctg caggtataga tgattttttt aaaggatggg agtcattatc aaaggatata
    38941 aatagtgaca gtaaaaaaag tctagtggta gaaaacgcat ctaatttagc aactttaata
    39001 agtgaaagtt ataagaagct tgataagtta aaagaagata tagattctaa tatagacact
    39061 gagataaaac aaataaatga catgttaaaa agtcttgaag atttaaataa atctattgat
    39121 attatatctg gttctggctc tacaccaaat gatttacttg atgaaagaga tagaatttta
    39181 gataacttga gttttaaact agactttgaa aatagtgatg ttaaaaatat gcttgcagat
    39241 ggtaaattag aacttaatga attaaaaaat ccagatggaa cttggaaaac aggtatatca
    39301 gggaccttac aaggcttgtt tgaaatgcat ggtaagattg atacatataa aagcgattta
    39361 aaagatgtat ctgatggatt agctaaacag ataaatgatg tttataatac gtctgcagga
    39421 ataacagtaa cagatttttt tataacttca aatgtagatg gtgaagatat tataaagata
    39481 aatccagcta taaagagtaa cccaaatgaa ttaaagctta ctgctgatga agcttcaaaa
    39541 attgctaagt taaaggatga aaaaatagat attggggtgg ctggtggaaa agtaagtaca
    39601 ataagtgacc actataaagc ctttgcagaa tcaatcggtt tagattctca aaaagtaaat
    39661 caagatgaag caaatcaacg taaaattata aataatattg ataactcaag aatgagtgtt
    39721 tcaggtgtat ctttggatga agaaatgaca gagcttataa aaatacaacg ttcatatcaa
    39781 gctagtgcaa aagttatgtc aacagctgtg caactactgg atgttgtaat aaatggaata
    39841 atttaataga agggggaatt tataatgaga gtcagtacag gtatgatgtc tagcagctat
    39901 ctaaactcac tacaagataa tctacaaaga ttggataagg ttaatagaca agttaataca
    39961 acaaaagaaa taaacaagct atcagataat ccatataaag caataaagat attaaactca
    40021 aaaagtgaaa taaagactat ggagacttat gttgagaatt gtaaagatac agggaattgg
    40081 ttagaaacta ctgatactgc attagatcag cttggaaaat tattatcaga cataaagaaa
    40141 actttggtgg caagtggtga tggtgcatac tcagatgatg aattaaagac tcttagtaat
    40201 gaaactaatg aaaaaatgaa agaaattgca aatgctttaa atgcaacaca tgaagggaag
    40261 tacatatttt caggttcaaa tacgggggta ccacctgttg aatgtataga taatgctgat
    40321 ggttcagtga gtttaaagtt taattctagt ctaaatctta ataaattaaa tgactctttg
    40381 agtatggata tagctcaagg aataagtgta gattataacg ttaaattgag tagccttgga
    40441 tttgacccaa caaaaactga cccatttgaa gagttaaata acatatcaaa aaaattagca
    40501 aatccagatg ctgatgctat aaaagagctt acaacaactt gtttgggcga tatggaacaa
    40561 ctaatagaaa atacagtaaa tgttaggtct atatatggga caaaagcaaa tacagtagag
    40621 gctatgaagg aaaaaaacga tgatagtttg atacagttaa aggatatttt gtcacaaaat
    40681 gaagaaatag attatggtga aaaacttgtt caattaaagg cagcagagtt aacttatcaa
    40741 gcttcactac aaacaggagg aaagttattt aatgtatcta tattagatta catataataa
    40801 aataacgcag atatgatgaa ggttacatta aaaaaaggta tattaggttt tgaaaattta
    40861 aaagaatatg agcttttaga tatagaagat aatgattatt taaaagaatt taattctact
    40921 gaagaagaag gtataggatt tgttgttgta tctccatttg agattatcaa tgaatatgaa
    40981 atagttttaa atcaggaaac aatagaaaaa ctagaagtga aatctccaaa ggatgttgta
    41041 ttatttaata taattactct tggtcagaca tttgaagaat caactgtgaa catgaaagct
    41101 cctatagtga taaatataaa aactaatttt ggaatgcaaa taattttaca ggatgataaa
    41161 tattcaataa ggcatccatt attgagaggt gatagtggat gctagtaatt tcaagaaaaa
    41221 aagatgaagc agtacttata ggagaaagca tagaagtaaa aattgtaggg gtagatggaa
    41281 acaatgtgaa actagctata tcagcgccta acaatataag tatattaaga aaagaaatat
    41341 acgaaaaagt aaagagtgag aatataaaag ctacaaataa aaatataaaa attttaaagt
    41401 cattaaaata ataagcttgg aggaagtaat atatgtatgg agaaaatcct tataattctt
    41461 ataaacaaaa tgctgttttt atggcatcaa aggaacaatt gttacttatg cttgttgatg
    41521 gagcagtaaa atataccaaa atagccaaaa cagctatctt agataaaaat acacaaaaag
    41581 cgcatagaga gcttataaga gttcaggata tatttacaga actaatggtt acattagacc
    41641 aaaatgcagg tcaatgggca aaggatatgt acagagttta tgattttgta agatatgaac
    41701 tttcaagagc taatattaga aaagacattc aaataataga taatgtgttg cctgtaatag
    41761 aacaaattaa agatacttgg catgaagctg ataaaaaaag taaagaagaa agaagtaggt
    41821 acaaataatg gaattaaatt tagctgaatt agataagtta tcaaaagagg aattactatt
    41881 gatgcttgta gatggaacag taaaatatac caatatatcc aaagaagctc ttttaaataa
    41941 cgactatttg aaagcacata atgaacttgt aagagttcaa aatatattta cagaactaat
    42001 gactacatta gaccaagatg caggtcaatg ggcaaaggat atgtataaag tttatgaatt
    42061 tataaaatct gaattagcaa tagctgatga aaataaagat attaaaataa ttgatgatat
    42121 attgccaata gtaaaacaaa ttagagatac atggcatgaa gtttacaatc aactaaaaat
    42181 ataattggat ggagggataa aggtatgtca agtataagtc ctataagagt tacaggtctt
    42241 tcaggaaatt ttgatatgga aggcataatc gaagctagta tgattagaga caaggaaaaa
    42301 gttgataaag caaaacaaga acaacaaatc gttaaatgga agcaagaaat atatagaaat
    42361 gttatacaag aatcaaaaga tctttatgat aaatatctaa gcgtaaattc tcctaatagt
    42421 atagtaagtg aaaaagcata ctcttctaca agaataacca gttctgatga aagtattata
    42481 gtagcaaaag gctcagctgg tgcagaaaaa ataaattatc aatttgcagt ttctcaaatg
    42541 gctgaaccag caaaatttac tattaaatta aattcaagtg aacctattgt tcaacagttt
    42601 cctccaaatg ctagtggagc gagttcttta actataggag atgtaaatat accaatatct
    42661 gaacaagata ctacaagtac tattgtaagt aagataaact ccctttgcgc agataatgat
    42721 ataaaggctt cttatagtga gatgacaggt gaattgatta tttcaagaaa acagactggt
    42781 tcgtcatcag acattaattt aagagtaatt ggaaatgaca atttagctca gcaaattgct
    42841 aatgataatg gtatcacatt tataaatgat gctggtggaa acaaagtggc aaatgtatat
    42901 ggaaagaatc tagaagctga tgtaactgat gaacatggaa gagtaactca tataagtaaa
    42961 gaacaaaatt catttaatat agataatatt gactataatg taaattcaaa aggaactgca
    43021 aagttgactt ctgtcactga tactgaagaa gctgttaaaa atatgcaagc atttgtggat
    43081 gattataata aactgatgga caaggtctat ggtttagtta ctactaaaaa accaaaagat
    43141 tatccgcctc ttacagatgc ccaaaaagaa gatatgacaa ctgaagaaat agaaaaatgg
    43201 gaaaagaaag ctaaagaagg tatacttaga aatgatgatg agttaagagg ttttgttgaa
    43261 gatattcaat ctgcattttt tggagatgga aaaaatatta ttgcattaag aaaactaggt
    43321 atcaatgaaa gtgaaaatta caataaaaaa ggtcaaatat catttaatgc agatactttt
    43381 tcaaaggctc ttatagatga tagtgataag gtatacaaaa cactagcagg ttattcttcg
    43441 aattatgatg ataagggaat gtttgaaaag ctaaaagata ttgtatatga atattctgga
    43501 agttcaactt ctaaacttcc taaaaaagca ggtatagaaa aaactgcttc tgctagtgaa
    43561 aatgtatatt caaaacaaat tgcagagcaa gaaagaaata taagcaggtt agttgaaaaa
    43621 atgaatgata aagagaaaag actttatgct aaatattcag ccttagaatc tttgttgaat
    43681 cagtattctt cccaaatgaa ttatttctca caagcacagg gtaattaatt atggataaat
    43741 taaaagaaaa attaaattta tataaagata tatctttaca aattataaat ttgattgaaa
    43801 aggaagaata tataaagata agtattaagt taggtgaaag acaagagatt ataaatagtg
    43861 taagtgaaat tgatagaaat gattttatac agctttataa tagaatggaa ttaattgaaa
    43921 ttgatagcag gataagagat attttacaag gacaattatt agaagtaaaa aaagaattac
    43981 atgagtataa gcttactaaa caagtgaaca caatgtatta taacttaaat agagaaaaag
    44041 taaatatttt taataaaaaa gtataaagtt atgtaccatc ttgtccgatt atataaatga
    44101 agaattaata aaaaagatta aaagtagaaa tgacaaggat gtcaactata ctaaggaggg
    44161 taaaataatg agagttaata caaatgtaag tgctttgata gcaaataacc aaatgggaag
    44221 aaacgtaaat gcacaaagta agtctatgga gaagctatct tctggtgtta gaattaagag
    44281 agctgctgac gatgctgctg ggcttgctat atctgagaaa atgagagctc aaataaaagg
    44341 tttagaccaa gcaggaagaa acgttcaaga tggtatatct gttgtacaaa cagcagaagg
    44401 tgcattagaa gaaactggta acatactaca aagaatgaga actttatcag tacaatcttc
    44461 aaatgaaact aatactgctg aagaaagaca aaagatagct gatgagttac tacaattaaa
    44521 agatgaagtt gaaagaatat caagctcaat tgagtttaat ggtaagaaat tacttgatgg
    44581 aagttctaca gaaataagat tacaagttgg agcaaacttt ggaacaaatg ttgcaggaac
    44641 atctaacaac aacaatgaaa taaaagttgc tctagtaaat acttcaagta taatgagtaa
    44701 agcaggtata acaagttcta caatagcaag cttaaatgca gatggaactt ctggaacaaa
    44761 tgcagctaaa caaatggtat ctagcttaga tgtagctctt aaagaattaa atacatcaag
    44821 agcaaaatta ggggcacaac aaaatagact agaatcaact caaaacaact taaataatac
    44881 tatagaaaat gttacagcag ctgaatcaag aataagagat acagatgttg cttcagaaat
    44941 ggttaactta tctaaaatga atatattagt tcaagcatca cagtcaatgc ttgctcaagc
    45001 taatcaacaa ccacaaggag ttttacaatt attaggataa aataattttg tatattggat
    45061 atagaagaaa gggtaaggct ttgctttact cctttctttt tttacaaata tacttttgaa
    45121 aggaaaattt atatgttact tagtatagtt atgattgtta aaaatgaaga aaaaatactt
    45181 gaaaaaactt taaaatcatt aaccacatta agaaattcta tagaaagtga acttataata
    45241 gtcgatacag gttctacaga tgatactata aaaatatcta agaagtacac ggaaaaagtg
    45301 tattttcata attggaataa tgatttttct agtatgagaa atatatcaat atcctatgct
    45361 aaaggtgaat ggttacttat attagatgca gatgaaatac taattgattc ttcaactatt
    45421 gtaaaatttt ttaaagatgg tcttgataaa aaatttaatt ctgcttcaat acaattaaaa
    45481 aatctttact catatgataa aaaattatat ggatattgtt cagtgcttag actttttaaa
    45541 aacgttgatt ttagatacca tggtaaagta catgaacaac caatatacaa aaaccctata
    45601 tttaataatg ttgctgattt tgaacattat ggatatttgt ttgaagatga agaaataaga
    45661 ttaaataagg tgaaaagaaa tgaaaaacta ttatttcaag agttaaaaga gaatgataat
    45721 tctccatata caaactatca attagggaaa aattttatta tattaggaaa atatcaggag
    45781 gctttatact atcttgagaa atcttatgaa ttatattcta aactagaatt ggcacctggt
    45841 tacttgataa caagccttgt aaaaacatat ttatatttag gaaaacataa gaagtgtgaa
    45901 agattgtgct taaagtatat taagaaagac atgaataata ttgatatata ttattatatt
    45961 gcacaagcac aagtagattt aggaaagtat gaaaatagta tagattcgta taagagatat
    46021 atttacttgt tagataatta tgaaataagt acacaagcaa atagtttatt ttcagacact
    46081 gatactgtag ggcttagaga tgaggctatt attacactga taaagattta ttataagctt
    46141 gaaaaatatg agtttgtaat ttcagagtat aataacattg aagatgtgga aaagaaaaag
    46201 aatgtgtact ttagcttatt tatgtcacta tataaattga atagatttga agatataaaa
    46261 aattattata aagaagtttc aaattctaaa attgaaaaaa atagttttta tagaaatata
    46321 gaagaagtta taaataatat taaagaggat gaaaaagagt atgtatataa aatcctttgt
    46381 gacattgaag gtaattatgg cattttaaat caaataagga taaataaatg tgtatctata
    46441 gataaatgta aagaaatact caatgaagaa aaggaaataa tttatgcata tattataaaa
    46501 atagcttttg aagagaagat tgatttacta gatatttttt atgaaatgga atatgtatgg
    46561 atagagaaat atctaaaata tcttttagca ttagaaagaa gttttaattt agtactatat
    46621 aagtacataa ttagtcgtcc aaatacatca gatatggaaa agataagagt atataaagct
    46681 atatctaaag tagttttaga aagtataagc ttaagtgatg aaaaatataa ggagattttt
    46741 tatctttata taatgtattc ttataagtac ataagataca tatatagcaa tttaagtgat
    46801 tttgaattaa taaaatttgt ttgtaatgat ttagataagt tcactataga atttaagcat
    46861 ctacttgata ttaaaaataa agtaaataat gctaatgaaa aattagagta tatacatagt
    46921 atgaggaaat tactaaatga gtatcctttt tataataaaa taataaaaat acttataggt
    46981 gaattagaag aaaatataaa agaaagtgaa gaatttaagg atttaaaaga tagttttatt
    47041 attagtatag aaaatatgat tgaaactggc aaaataaatg atgcgaaatt attggtagat
    47101 gactattcaa aaacatttgt ggatgatata aagattttaa atataaaggg aattttatac
    47161 atgtttgaaa ataagcttga taaagctgat tttatgttaa aaaaggcatt atcattagat
    47221 gtagaaaatg aagatactat atataatata gaatacttaa aaagtttaag ataagtcaaa
    47281 aataggagaa ttgaagaatg aatataccaa tagtggttaa tgaaaaatct aaagacataa
    47341 taaaagattg gcaatataca tgtacaaatg aagatataaa atttacaagc ataataatat
    47401 tgacatataa tcaaatagaa tatactaaat tatgtattga aagtattagg aaatttaccc
    47461 caaagaataa atatgaaata ataataattg ataataactc ttctgatgga acagttgagt
    47521 ggttaaaggg acaatctgat ataaaatctg tgtataataa caaaaactta ggatttccaa
    47581 aaggatgcaa tcaaggtatt gagttagcta ctggtgaaaa catattgttg ttaaataatg
    47641 atgtaatagt tactccaaat tggctctata atttgaataa agctttatgg tctcaagata
    47701 atataggtgc tgtaggacct attacgaatg catgttcata ttatcagcaa attgatgttg
    47761 attataagtc aattgaggac atgtttatat ttgcacacaa aataaataaa tctaatatta
    47821 acctatggaa atatagattg aagttagttg gattttgcat gttaataaaa aaaactgttc
    47881 tggataatgt aggggtttta gatgaattat tcacacctgg aaattttgaa gatgatgatt
    47941 tatcttttag gatgatagag tcaggatata agttattatt atgccaggat acttttatac
    48001 accattttgg aagtgtgtct tttaaagaaa atcgtaataa atatacagat ataatgtata
    48061 ttaatagaag aaaatttgaa actaagtggg gatttaatcc agaatatagt ggaatgataa
    48121 gaacagattt gatttctaaa atagatgata gtttggaggc aaatattaat gttttagaaa
    48181 taggatgcgg tataggggct actcttttag aaataaaaaa tatatataaa aatgcatcaa
    48241 tatatggaat agaaattagt gagtcagcag gtaaacttgc aaagtatagt gcagatgtac
    48301 ttatagcaaa tatagaaaaa gtaaaattgg attataaaaa agattttttt gattatataa
    48361 tattgggaga tgtacttgaa catttgattg acccatggaa gatagtcaat gaattgaaac
    48421 aatatctaaa gaaggatggt tatctggtat ctagtatacc aaatattatg catgtttctg
    48481 ttttaagaaa tcttgtgtta ggtaatttta catatgaaga ttcaggaatt ttggacaaga
    48541 ctcatttaag gttttttaca ttaaatgaga taacaaaaat gtttaatgat agtaattaca
    48601 taattaatag tattacagct actagtatgc cactttcgga aattgataat agcttcattg
    48661 atacaatatg taaggaaagc tgtaaagaaa tgagacaaca gtacgaatct tatcaatata
    48721 tagtaaaagc ctctaaaatc ctagatacta aaagatattc tagtattagt atgattaatt
    48781 taaaatacgc tttaatgaga attgataatg aattagatgt agatagtaat ttaagttata
    48841 tatttgataa ttataatata actgaaccat gttttgttga tgatattgaa tatctaattg
    48901 atacatttgt gataaataaa gcaactctat taaatagatt aggagttgag gcatttaaca
    48961 gaggtctagg agattttagt ataaacatgt ttatgacagt attagagatg gatagagatg
    49021 atattgactc aatatataat ataacaagtg tattatgtga attagaagaa tttgatactg
    49081 cttataattt aattaaaaat agtagcgaaa aagtacagag tgaaagtgat atacaagaga
    49141 tactttgttt tattgagggg aagaaatatg aataatgata agatatgttt tataacctgt
    49201 gtaaatgatg aaaggcttta tgaagaatgt cttgtatata taaatagcct agacattcct
    49261 aaaaacttta gtatagaaac agtagcttta agaggagccg attctattgc ctctgcttat
    49321 aataaagcta tgagagaaac agattctaag tataaggtat acttacatca agatactttt
    49381 ataattaata aaaatttcat acatgatatt ttagagatat ttaaaaaaaa tacagatgtc
    49441 ggaatgattg gtgtagctgg agtaaaagat atgcctgtga atggaatttg gtgggattct
    49501 gataaaaaag cagggaaagt atatgatagt catacaggta aaatggatat ttatgaattt
    49561 agtaactttg aagaatgttt taatgaagtt atggctatag atggattaat tatgattact
    49621 caatatgatg tattttggag agaggataag tttgatgggt ggcattttta tgatttgtca
    49681 cagagtatgg agtttagaca aaataaatac aaagttattg taccaaaaca aaataagcct
    49741 tggtgtatgc atgattctgg agtagtaaat ataaataatg gatatgatga atataggata
    49801 aaatttgtaa aagagtacat aaatcttgat ttatatagag aaaattttta tgaatttgat
    49861 aagaagaaag caagtaccat acttaagagt aataagcatg taatggattg tatgaaagag
    49921 gagcttacag aacaattaag attaaagaat tacaaggatg ttttaggtat tgtttctaaa
    49981 attgctcatt tttctgtttt taatcatact ggtttgtatt cttgtgaaga atgtgagaga
    50041 gcattgctag aatgtgcaaa taatttacca gaaatagaaa atacgaaatt atttacaaaa
    50101 aaagatttaa aaagaagaaa agtattacat gttttaagtg aaggatattc aacaggaggt
    50161 catacaagac tagttaaaaa ttggattgaa attgacaaag actctgttca ttcagttgta
    50221 actacatggc aatcagatac actgccagac tggttattaa aagcaataaa tgatagtggt
    50281 ggcgaagtta tatctttgga tttaatttca gatgattatt taaaaagagc tgtagctttg
    50341 aggaaaatag cttatgactg ggctgatata gttattttat atgttcatat gtgggataca
    50401 atacctgttg ttgcttttgg agttgatggg aaaataccta ttttacatgt taatcactat
    50461 gatagtactt tttggttagg gacaagtatt gcagatattg tcattgattt tagcgaaact
    50521 agtcaattaa ttacattaga taaaagaggc atagataaat ctgaaatatt accaatgcca
    50581 ttaaaagaaa aagaagtatt ttcaaaatct gaagttagaa aacgatataa tttagaggat
    50641 gatgtgacaa taatattgac tattgctagt gaaagaaaat acaatagtgt aaatgatatt
    50701 aattacttta aaattgctaa aacattgatt gaaaatacag ataatacaat tattattgtt
    50761 gtaggagttg atacagagaa tctctattgg aaaactttaa gtaatgttac agataataga
    50821 gttatatgtt ttggaacaac agcaaatata gatgattttt ataagatttc ggatatatac
    50881 ctagagtctt tcatggtagg aagtcatact tcaaaattag atgcaatttt atatgattta
    50941 attcctgtta agtttaaaaa tgaagtttca ccaattttta caaatatgtg gaattttata
    51001 gataagtttg attataagaa tataaaagag gttatagaca tgataagaag gtacaatact
    51061 agtgaatttg acagggataa gataaaagca aaacaatcgg aaataaaaca ttttgttata
    51121 gataaacatt gtattgaaat aaaaaactat atagacaaaa tatataatga agttaatcat
    51181 cataaagtta agtatgattt atataaaaat gatgaaatcg aggactatca gctttttgta
    51241 gcattatatg gctatatggt aaaaaataaa aacatgtcat aggtggtaaa atgaacaata
    51301 ttcatatcaa taaatttgaa aaaagctgtg aaaaaaactg ggattatttt gtagaaaata
    51361 aatctataaa tggtacattt ctgcaaacta gaaatttcct taattatcat gaggagggaa
    51421 aattcactga ccactcatta ataatatgta aaggtgaaga aatactagca gtcgtacctg
    51481 catgtgaaac ttatgaagat ggtgaaagaa tatttttttc tcatttaggg agtacttttg
    51541 gtggcattat aataagaagt ggttttaatg atattaggca tgtaaatgcc attacaaata
    51601 agttagaaat atatttacag gaacaaaatt tcgataaagt atatttaaaa aatacatcaa
    51661 gaatattttg taaagaggat acttcattac tagaatattt tttatttaag aataattaca
    51721 tgttttatga tgaattaagt atgtttttag aattaagtaa gtataaagaa gatatattag
    51781 ccaattttaa atacagtaaa agaagagatt ttaaatattc tttgaagaat aatcttacct
    51841 ttaaaagact gtatagtgat gaagatatag tttgtttttt taatttactt tctaattgtt
    51901 tagatagtaa aggagctaaa gtaatacatt cagtagaaga acttttggag tttaaaaata
    51961 atagattaaa agatattgta gaattttatg cagtatatat tgatgacatt atgatttgtg
    52021 ggagcatggt atttaacttt caagaaagag tttatcatac tcaatatcta gcatcaaatt
    52081 cgacatataa taaatattat ccaatgatat ttttatacta taatctaata aaagatgctt
    52141 tagagagaaa ttttaagtat ctttcttttg gaattagcac tctaaataga ggcaaatact
    52201 tagatgaggg tttagctaca tttaaagaag gttttggttc aagtgcaatt tgtaacagaa
    52261 catactataa aaatataaat aagtgagaat catgttttat agagttttta gagtatagta
    52321 aatatctagt actataatat tacataaaaa taattagttt ataaagaagg tttgtatatg
    52381 aaaagattaa tgattttagg ctcaattaag tattttgaga atattgttat gtcagctaaa
    52441 agagagggaa tatacacaat tgtttgtgat aatagaattg atactcctgc gaaaaagatt
    52501 tgtgatgaag caatagatgt tgatgtattt gattttgata aaataaagaa tatagctata
    52561 gataaaaaga tagatggaat tttaactgga tttacagatt ctttaatgaa gccatatgtt
    52621 tatattgcaa atgaactaaa tttgccatgt gtgatttctt gcaatcaact tgaatctgta
    52681 actaataagg ctgttatgaa aaattatttt aaaaataatg gaatacctac aactgattat
    52741 tgtattattg aaagtttgaa agatttagaa aaaatacaag gattaaattt tccacttgta
    52801 ataaaacctg ttgatggtta tggttcaaga ggtgtatatt ttgtatcaaa catagaagaa
    52861 ttaatagaaa aatttgaata ttctaaggaa gcctcgcaag atggacaagt aataattgag
    52921 gaattttatg aaagtgagga aatacaagga ctagcttggg ttcataatgg agagtcacat
    52981 gtattttata ttggagatag agagcttgta aatatacata caggaagagc tggaaaacca
    53041 gatagattaa tttatccatc aaagtattgt tttcaatatg aagaagaaat aaaatccata
    53101 tatcaaaata taacgcaggc ttttggcata aaaaatggac ctctttatgt acaaatgtta
    53161 gtgggagcat atggaataaa ggtatctgaa gtaatgccaa gattacctgg aggttgtgac
    53221 tatatggcta tttctcaaat aacagattta gacataggaa gtttatttgc aaactttagt
    53281 gtgaataatg aagtaaattt tgatgaaata aagaaatata atatgcatct atcaaaatgt
    53341 gtatatgcat taccaatata cataagacct ggcacaataa aaaagataaa taatatagat
    53401 aaaatagaaa acttagatta tgtcactcaa tttctattac tagtaaaaga aggagataca
    53461 ataggattat atgaagatga gagacaggat tgtggaagag tttatggagt agcaaataat
    53521 gtatttgatg caaatataaa gaagaaatgt attcatgaaa tgatagaaat acttgatgaa
    53581 aataatgaaa atatgataga aaatttttaa ataagggagt atgatagata tgaagctaat
    53641 aactcatgaa caaattatgt cattaaacat ttctcagaaa caaggatacg agtggataaa
    53701 agaatccttt ttaacaaaaa aagattgtca attaccacca aaaataagca tggtagaaga
    53761 agggcatgta ttttacaatg taatgccgtc cctaaatcat aatcataaga ttggtggaat
    53821 aaaaatggtt acaagattgc cagggagaaa tccaagttta aattcagaga tatttctata
    53881 taatatggaa gatgctaaat taaaggctat tttagatggc aattatatta cagcttttag
    53941 gactgctgct gttgctgcct tggctatcga tacatttgct aaaaaagatt ttaatactat
    54001 tgctattatg ggtttaggtg taactgcttc tgctataatg aatatgcttt catatactct
    54061 taaagataaa aatataaata taaagttact taaatataaa gaccaagcag agtcgtttgt
    54121 aaaaaagtat gaaaaaaatg aacaatttag ttttgaaata tgtgacacac atgaagaact
    54181 tattgtagat tcagatgtag ttatttctac tgtgacatat acagatgaat atttatcaga
    54241 atttaaatgg tttaaaaaag gtgctttatt attacctgta catgttatgg gatttggaaa
    54301 tgttgattat ctatttgata aagtatattt ggatgattat aatcatttga aacatctaga
    54361 aaatatagat agatataaat attcttgtga attttatgat gttttaagta aacaagctaa
    54421 aggaagagaa aatgatgatg agagaataat atcttacaat ataggtttgt caatacatga
    54481 tatattattt gcaaataaaa tttacgaatt gtgtgaaaca ctaagctgtg aagaaataga
    54541 attaaaagaa ccaaaagaaa aatattggat atagtttatt tcattcatat ttacaatagt
    54601 ttattgtaaa tatgaatatg aagtaatata ttgttagaaa attagcatga ataggataga
    54661 taaatttatg gataaatata atttaattag gttcgtatct attggaaacg aatatatagg
    54721 aaatttagtt gcattagaat caaataaagc aatacctttt gatataaaga gagtttatta
    54781 catatatgat gtaccaaaca atgtaaagag aggatttcat gcacacaaaa acttggaaca
    54841 agtactagtt tgcctaaatg gaagtgtgaa gataaaatgt tatgatggtg taaaagagac
    54901 tatatttaat ttacataaaa atgatgttgg attatatata ggaacgggag tgtggcatga
    54961 gatgtttgat tttgatgaaa aaactgtttt aatttctata gcatctgaac attataatga
    55021 agaagattat attagagact ataaagaatt tttaaagtat ttaaataatg tgtagttatt
    55081 atacaattag tgatgtattt tcttaattaa ataaatttaa gatagccaca tagagaatag
    55141 atagattagg atgtgaattt attgttggtt aaatttaata atttattaaa acagttccaa
    55201 actcataaag aagaatatga acagaagatt aaggatgtaa tggaaagtgg atattatatc
    55261 cttggaaatg aagtaaagca ttttgaagaa gaatttgcta actatgtagg tagcaaatat
    55321 tgtgttggtt tagccagtgg tttagatgca ctgataattg cttttagagc attaggaata
    55381 agcaaaggtg atgaagtaat cgtgcaatca aatacatata ttgctagtgt tatgggaatc
    55441 agtataaatg gagcaacacc agtatttgta gaaccaaatg aatattataa tatagattca
    55501 tctaagatag aagaaaagat aaatagcaaa acaaaagcta tattagtagt gcatttatat
    55561 ggtcaagcaa gtaatatgaa agatatacaa gaattatgtg ataagtataa cttgtattta
    55621 attgaagatt gtgctcaatc tcatggagct aaatatgatg gaaaaatgac aggaacattt
    55681 ggtgatatag gttgttttag tttttatcca agtaaaaatt tgggtgcatt tggagatgca
    55741 ggagctatag taactagtaa tgaggaaata gctgataaag tgaaaattct tagaaactat
    55801 ggctcagaaa agcgatatta taataaattt gtcggatata attcaagatt ggacgaaatt
    55861 caggcaggtt tattaagagt aaaacttaaa tatctaaatg aaattgaaat tgaaaaatca
    55921 aaaatcgtag atagatatct taatgaaatt aataatccca aagtcatact tccaaagata
    55981 gaaaataatt gtactcatgt atggcattta tttgttttac aagttgaaaa acgagataca
    56041 tttatggaat acttaaagaa aagtggtata gagtctataa tacattatcc gataccacca
    56101 catctatctg aagcatatgc ttatttggga tataaaaagg ggagttttaa tatagctgag
    56161 aactattcag ataaagttgt tagtatacct ttatttattg gtatggaaga tgaagaagtt
    56221 agttatataa ttaaaattat aaatgaatat taaactactc ataataatat caaaaataaa
    56281 ttttttatat atcatttatt taaatttcgt taattgacat ttttaaatat tgatatattg
    56341 tacaaataaa attgaaatat atg
//
LOCUS       DAEPIN010000018        56220 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.018, whole genome shotgun sequence.
ACCESSION   DAEPIN010000018 DAEPIN010000000
VERSION     DAEPIN010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 56220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56220
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.018"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            <1..196
                     /locus_tag="KR250_001297"
     rRNA            <1..196
                     /locus_tag="KR250_001297"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            324..440
                     /gene="rrf"
                     /locus_tag="KR250_001298"
     rRNA            324..440
                     /gene="rrf"
                     /locus_tag="KR250_001298"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            448..522
                     /locus_tag="KR250_001299"
     tRNA            448..522
                     /locus_tag="KR250_001299"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:480..482,aa:Asn,seq:gtt)
     gene            528..613
                     /locus_tag="KR250_001300"
     tRNA            528..613
                     /locus_tag="KR250_001300"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:562..564,aa:Leu,seq:taa)
     gene            629..704
                     /locus_tag="KR250_001301"
     tRNA            629..704
                     /locus_tag="KR250_001301"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:662..664,aa:Met,seq:cat)
     gene            712..786
                     /locus_tag="KR250_001302"
     tRNA            712..786
                     /locus_tag="KR250_001302"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:745..747,aa:Glu,seq:ttc)
     gene            801..874
                     /locus_tag="KR250_001303"
     tRNA            801..874
                     /locus_tag="KR250_001303"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:833..835,aa:Gly,seq:tcc)
     gene            880..955
                     /locus_tag="KR250_001304"
     tRNA            880..955
                     /locus_tag="KR250_001304"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:913..915,aa:Val,seq:tac)
     gene            961..1037
                     /locus_tag="KR250_001305"
     tRNA            961..1037
                     /locus_tag="KR250_001305"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:995..997,aa:Asp,seq:gtc)
     gene            1048..1122
                     /locus_tag="KR250_001306"
     tRNA            1048..1122
                     /locus_tag="KR250_001306"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1080..1082,aa:Gly,seq:gcc)
     gene            1132..1205
                     /locus_tag="KR250_001307"
     tRNA            1132..1205
                     /locus_tag="KR250_001307"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1166..1168,aa:Arg,seq:tct)
     gene            2189..3028
                     /locus_tag="KR250_001308"
     CDS             2189..3028
                     /locus_tag="KR250_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HBH0067256.1"
                     /translation="MLDINSFLNGFFNIILRMSIYDLIDISIVAYIFYKIFMFIKDTR
                     AEQVFKGIIFLLLATQLSNTFKLHTVYWISLKALDYGVIAALIIFQPEFRAGLEHIGR
                     AKFNLFGKNVNTSEETLNRNIEEIVEALYSLSRQKIGALIIMERETRISDIINTGTII
                     DAEISRQLLINIFIPNTPLHDGAVVIRDSKVKAAACFLPLTESKDLSKDLGTRHRAGI
                     GVSEVSDCITLIVSEETGGVSIAKAGKLYRDISRERMMNILRSNLKTNTETRSFFKGG
                     IFK"
     gene            3025..4206
                     /locus_tag="KR250_001309"
     CDS             3025..4206
                     /locus_tag="KR250_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0067257.1"
                     /translation="MINRLKNNTKIKIISFLSAIALWMYVMAVVDPEETKGFEDIPVS
                     ISNMSDLKDKNLVIYPDEKLTADVYIKGSLSKVQSVKKEDIHVYGTIEDPIQGQKEVY
                     LKANIPQGVTLEFKHDVLVVNLEKNIKEKRDIKVEVSGNSKRNINKIELSKEFVDVSG
                     PRTLVKEVKSVRANLDVGNETDDFSKKLQLIPLNSDGTKVEGVTLSSSFVTAKIELLK
                     EKIVPIRVSLNDNKDNDVLKNYKLSKEQVTIKGKKEDIDKISYIKTKPIDLADLANGE
                     EKEVSLEIPEGITLEDSSITIKLNDTKQLSAEFLYNAGDIELRNIPTNMDNPNVISDV
                     DIKVVVESTEDLSKLNKKDISLYIDLSQGPEEDSKYKIKYETTYQFKKINIEPNIVEV
                     E"
     gene            4510..5412
                     /gene="ptb"
                     /locus_tag="KR250_001310"
     CDS             4510..5412
                     /gene="ptb"
                     /locus_tag="KR250_001310"
                     /EC_number="2.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate butyryltransferase"
                     /protein_id="HBH0067258.1"
                     /translation="MRSFEEVIKFAKERGPKTISVACCQDKEVLMAVEMARKEKIANA
                     ILVGDIEKTKEIAKSIDMDIENYELIDIKDLAEASLKSVELVSQGKADMVMKGLVDTS
                     IILKAVLNKEVGLRTGNVLSHVAVFDVEGYDRLFFVTDAAMNLAPDTNTKKQIIENAC
                     TVAHSLDISEPKVAAICAKEKVNPKMKDTVEAKELEEMYERGEIKGCMVGGPFAIDNA
                     VSLEAAKHKGINHPVAGRADILLAPDIEGGNILYKALVFFSKSKNAGVIVGAKAPIIL
                     TSRADSEETKLNSIALGVLMAAKA"
     gene            5434..6513
                     /gene="buk"
                     /locus_tag="KR250_001311"
     CDS             5434..6513
                     /gene="buk"
                     /locus_tag="KR250_001311"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HBH0067259.1"
                     /translation="MSKIFKILTINPGSTSTKIAVFDNEDLVFEKTLRHSSEEIGKYE
                     KVSDQFEFRKQVIEEALKEGGVKTSELDAVVGRGGLLKPIKGGTYSVSAAMIEDLKVG
                     VLGEHASNLGGIIAKQIGEEVNVPSYIVDPVVVDELEDVARISGMPEISRASVVHALN
                     QKAIARRYAREINKKYEDINLIVAHMGGGVSVGAHKNGKIVDVANALDGEGPFSPERS
                     GGLPVGALVKMCFSGKYTQDEIKKKIKGNGGLVAYLNTNDAREVEERIEAGDEKAKLV
                     YEAMAYQISKEIGASAAVLKGDVKAILLTGGIAYSKMFTEMIADRVKFIADVKVYPGE
                     DEMIALAQGGLRVLTGEEEAQVYDN"
     gene            6503..7180
                     /locus_tag="KR250_001312"
     CDS             6503..7180
                     /locus_tag="KR250_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBH0067260.1"
                     /translation="MITNDKRIRIITGHYGSGKSEFAMNYVVKLRDMVSGKVAIADLD
                     VVNVYFRTREKKELMKSLGIQPIDSSINAPTLDLPAVSAEVMSPMVDHSYNSVIDLGG
                     DNVGARVIGRFSHLLKEGDYDMLFVINANREKTQTSEEVIQYIKEIEKSSKLKVTGLI
                     NNTHLIRFTTIDDVLRGQKVAKEVSEKCNIPIRYVACLENLVEQLPKDLEGEIFPIKL
                     YMREDWM"
     gene            7223..7438
                     /locus_tag="KR250_001313"
     CDS             7223..7438
                     /locus_tag="KR250_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBH0067261.1"
                     /translation="MAKGKVSFNQERCKGCGLCVEACPVKIIQLDSNVINKKGYNPAT
                     VFEMEKCIGCASCATMCPDVVITVERD"
     gene            7467..8546
                     /locus_tag="KR250_001314"
     CDS             7467..8546
                     /locus_tag="KR250_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HBH0067262.1"
                     /translation="MAKILMKGNEAFGKAAIEAGCKYFFGYPITPQSELPEYLSRELP
                     KIGGAFVQAESEVSAINMVYGGAGAGARVMTSSSSPGVALKQEGITYAVGAEVPCVVL
                     NVMRGGPGLGSIQPSQADYFMSTRGGGNGDYRTPVFAPATVQEAVDMIMEAFDVADYY
                     RSPVMVVADGMIGQMMEPVEFRAPEKKRELPPKDWATVGTKGKRKPNVINSLYLEPEV
                     LEDHCWHLQEKFDAMEKNEVQYEMYKTEDAEFVFAAYGTTSRVVKSAIDILREEGIKA
                     GLIRPKVLWPFPFEAFNQIPNARNILTVEMSMGQMVEDVKMAVEGKLPVYFHGRPGGM
                     TPTPAEIVEKAKKIIAGELVAGGAR"
     gene            8546..9298
                     /locus_tag="KR250_001315"
     CDS             8546..9298
                     /locus_tag="KR250_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HBH0067263.1"
                     /translation="MAVVFKKTEGLQDTQTHYCPGCTHGIIHRLVGEVLEELGVLGDA
                     VGVVPVGCSVLGYKYFNCDTQEAAHGRAPAAATGIKRVHPENTVFTYQGDGDLASIGT
                     AEIVHAAARGEKITTIFVNNTTYGMTGGQMAPTTLVGQRATTAQSGRNAETQGYPIRV
                     SEMLATLTGAVFVERVAVDTPAHVRQAKKAIKKAFQVQQAGLGFGIVEVLSTCPTNWG
                     LAPNDALQWLRDNMIPYYPLGNFKNVEVEEVK"
     gene            9299..9856
                     /locus_tag="KR250_001316"
     CDS             9299..9856
                     /locus_tag="KR250_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HBH0067264.1"
                     /translation="MSTARVICAGFGGQGVMSMGQLLTYAGMLEGKEVSWLPSYGPEM
                     RGGTANCAVTVSNEPVGSPLITDDATAAILLNIPAFEKFKDDVIPGGKIIVNSSLIKE
                     KVDRTDVDVYYIPANELAAELGNDKVANMIMLGAYLKVSDTVDIESVLEAFKKVFGPR
                     KEKFVPLNREALQKGMDATCSKATN"
     gene            10026..11372
                     /locus_tag="KR250_001317"
     CDS             10026..11372
                     /locus_tag="KR250_001317"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HBH0067265.1"
                     /translation="MRKYFGTDGVRGVANTELTCDLAYKLGRAGGFVLAQGDHRVKVV
                     VGKDTRISGDMLEASLIAGLMSVGCDVITVGIIPTPAVAYLTRKYGADCGVVISASHN
                     PVEYNGIKFFNKNGYKLDDEIELKIEEYIDDIDKIDCLPIGENVGRKLHEHCAQRDYV
                     DYLKSIISTDFKGLKVVLDCANGASYKVAPIVFDELGASVISINSSPDGNNINYKCGS
                     THPEQLQRAVLEHNADLGLAYDGDADRLIAVNEKGQIVDGDHIMILSALNLKKNNKLA
                     QDTLVVTVMSNIGLTIAAKENGINLSTTAVGDRYVLEDMVKNGYNLGGEQSGHMIFLD
                     YNTTGDGVLSSLILANIILQEKKPLSEIASIMSQYPQVLVNATIKNENKNKYMEYPEI
                     KTEIERIESILDGNGRVLIRPSGTEPLVRVMLEGKEEGQIKELATNLANLIQEKLS"
     gene            11639..13471
                     /gene="glmS"
                     /locus_tag="KR250_001318"
     CDS             11639..13471
                     /gene="glmS"
                     /locus_tag="KR250_001318"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003401499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HBH0067266.1"
                     /translation="MCGIVGYLGSRKAAEVIVEGLSKLEYRGYDSAGVAVNSSNEKEL
                     NIRKFKGRLSVLAEDLEKNPIDGNLGIGHTRWATHGEPSDVNSHPHFNQAKTIAVVHN
                     GIIENYMEIKEELISEGVKFESQTDTEVIAHLVDKYYEGNLLDAVYKTISKLRGAYAL
                     GVICKEHGNELVAVRKDSPLVVGVGEGENFIASDIPALLKYTRDVYFLENGEVVHLKD
                     ENVTVYDSNRNLVEKEVFHVTWDVEAASKGGYDYFMSKEIHEQPTGVRETLERRLDDN
                     GNIILDSINISKEDLEKINKVYIVACGTAYNAGLLGKYAIEKFVNIPVITDIASEFRY
                     SDPFVDENSLVILVSQSGETADTLAVLRDSKAKGARILSITNVVGSSIARESDDVFYT
                     WAGPEVAVASTKAYTTQITSLYMIALDFAIKKGTITREFYDSMISKMKEIPSKIQEIL
                     DNEEYIKEVAKTVVSSEHAFYLGRGIDYSLAMEGSLKLKEISYIHAEAFAAGELKHGT
                     IALIEKGTPVIAIATQEKLFEKMVSNMEEVRARGAYVVAIAQSHNKDVEKAADKIIYI
                     PNSDDILSPILAVVPMQLLAYHVSVLRGCDVDKPRNLAKSVTVE"
     gene            13922..15217
                     /locus_tag="KR250_001319"
     CDS             13922..15217
                     /locus_tag="KR250_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase"
                     /protein_id="HBH0067267.1"
                     /translation="MTNDFKKEKKEDYFVNLKAISTEVLQEKVQDNAWVIVDTRLNDA
                     YNGWKLDGVKRGGHIKGAVDFSANWLSVYSDRKDEVLEQALKTKRIDLDKNIVLYDAN
                     GKDALVVADYLSKKGYKYLYKYDIKQWADNENLPMERYKNYQMIVPAFIIKDILDGKI
                     PETFEDSKNIKMIEASWGEESYTKGHIPTSVHVNTDIIEPPPTWMLDNDDNLIKFALD
                     YGLTKDDTVIVSSSTPMASYRLAVILRYIGVKDVRVLNGGTNSWLSAGYELEFTSNPK
                     HSCTNFGADIPVNSQLVVTTSELRQKLKEKNKFILVDNRTWDEHIGKVSGYTYYDKKG
                     RIPGALYGHSGSDSVSLEEYRNIDNTMRNKYEILEMWDKENIDVNRQLIFMCGSGWRA
                     AEVLTYANVIGVENTSLYSDGWMGWSLDNSNLIEVGEHK"
     gene            15667..16404
                     /locus_tag="KR250_001320"
     CDS             15667..16404
                     /locus_tag="KR250_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwmB family TATA-box binding protein"
                     /protein_id="HBH0067268.1"
                     /translation="MKRVKLAISFFILMIIGMTISYADTSINTKYNRLSKALEISEAN
                     FKFYNVKINSVINHNLSNKEFHNIFIEIINNLKLDKKGINWNQKGSGDKIQLCATIKG
                     SDKEISIKGINKNNKESYIVVDILDNKVYKHKEVIYSNVENVLNKYSSQVDVSACIVG
                     EYTKRLQNNKYDDILQKILYNMSAKQIDEVKDVNFLSITAYSKVLNDYELEYLGEKIN
                     LNIGIKYNEDDGRTLIYIATPIIKLDY"
     gene            16425..17678
                     /gene="murA"
                     /locus_tag="KR250_001321"
     CDS             16425..17678
                     /gene="murA"
                     /locus_tag="KR250_001321"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HBH0067269.1"
                     /translation="MAKIIVKKSNPLKGSVKIDGAKNAVLPIIAATLLANGKSTLNGV
                     PNLRDVHVISDLLRHVGAEVEYKENTLTVDASNIKTCEAPYELVRKMRASFLVMGPLL
                     ARFNSTKISMPGGCAIGTRPIDLHLKGFKALGAKIEMDHGFVEAATEKLVGNKLYLDF
                     PSVGATENIMMAASLAEGTTIIENAAEEPEIVDLANFLNEMGADVKGAGTNTIKIKGV
                     KELKGAEHNVIPDRIEAATYMVAAAMTKGDITVENVLMEHLKPVVAKLREAGCEITEM
                     DNSVRVVGPKVLKPIDIKTLPHPGFPTDVQAQFMAMLTVANGTGVVIETVFENRFMHV
                     AEFNRMGANIKIDGRSAVVNGVDELHGAAVNATDLRAGAALILCGLIAEGETQIGEIY
                     HIQRGYVDIDKKITALGGQIEIVED"
     gene            17765..18829
                     /gene="spoIID"
                     /locus_tag="KR250_001322"
     CDS             17765..18829
                     /gene="spoIID"
                     /locus_tag="KR250_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein D"
                     /protein_id="HBH0067270.1"
                     /translation="MKNPLVVLLGFVTCSVLVPSLITLVSYKNVELTEKAESPVSINK
                     TIKKSDIEDKGNKEEKNTVNYETVNKKAPIINVYNHITGKTEKMDMENYLCGVLAGEM
                     SSEFDIEALKAQSVAARTYVVYKQEHGKSSKHKNAVVCTDYKHCQEYKSYDTLKKLNG
                     EEWIKNKYSKIQEAVRGTKGQIITYNDKAILPLYFSTSSGKTENSEEVFSAKYPYLKS
                     VESPYDKYSPKFASTLKISNTDFVKSLRRAYSTIVIDVNNLSKQVSITKRSDAGTVEK
                     IKLGNKELTGKDIRTVFKLNSANFDIKFGEGYIDFVVKGYGHGVGMSQWGAEGMAEEG
                     YKYYDILSHYYTDTKIKDIY"
     gene            18895..19563
                     /locus_tag="KR250_001323"
     CDS             18895..19563
                     /locus_tag="KR250_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HBH0067271.1"
                     /translation="MKKKLLEKDGFYLSLFVCVCLLAVGGVWFTNNNVDKLASNKGIM
                     ENANKDSEEEIHLIEKDKKDAIPTATDSKQNLEKAKSKEENKSSTTKLNYIGDKVIRG
                     YSEKEPSYSKTLDVWETHKGVDISCTKGKEVKSLLNGIVVDVFNDEEYGQSVKIKSDN
                     NIVVVYSNLDKNVSVKKEQKVTEGQSLGTVGSTSQIESEEGIHVHLEAYSGEKSIDPM
                     SLIK"
     gene            19661..19921
                     /gene="spoIIID"
                     /locus_tag="KR250_001324"
     CDS             19661..19921
                     /gene="spoIIID"
                     /locus_tag="KR250_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcriptional regulator SpoIIID"
                     /protein_id="HBH0067272.1"
                     /translation="MRSHIEERAIVVAKYILEKNTTVRQTAKTFGVSKSTIHKDVTER
                     LKEINPSLAKEVKNVLDKNKSERHIRGGLATKLKYEKEHKKM"
     gene            20118..21137
                     /locus_tag="KR250_001325"
     CDS             20118..21137
                     /locus_tag="KR250_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HBH0067273.1"
                     /translation="MAGADIGIDLGTANVLVYVSGKGIVLEEPSVVAIDNRTDSVLAV
                     GEEAKKMIGRTPGNIVAIRPLRDGVISDYDVTEKMLKSFIDKIVDKKGFGRFFMPRIM
                     VCVPTGVTEVEKRAVEDATRQAGAREVYIIEEPIAAAIGAGVDISQPNGNMIIDIGGG
                     TVDIAVISLGGAVVSESIKVGGDRFDDAIVKYMKKQHNLLIGERTAEKIKFEIGSAFK
                     REEEKYMKITGRNLITGLPSSITINSTEMLEALRECVEQIVVATHAVLEKTPPELAAD
                     IGDVGIIMTGGGSLLYGLDKIIEQRTGITVTIADEPLSCVAKGTGIALGSIDLLETGG
                     SFKRK"
     gene            21153..21581
                     /gene="fabZ"
                     /locus_tag="KR250_001326"
     CDS             21153..21581
                     /gene="fabZ"
                     /locus_tag="KR250_001326"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HBH0067274.1"
                     /translation="MLNIDEIKKLIPHRYPFLLVDKITELEVGKRAVGIKNVTVNEPF
                     FQGHFPEYPLMPGVLIVEALAQVCGVAMMSVEENKGKLGVFAGIDKVRIKREVRPGDT
                     LTMEVEMTTLRRNIAKADAKAYVGEELVCKGELMFALVEK"
     gene            complement(21692..22201)
                     /gene="yyaC"
                     /locus_tag="KR250_001327"
     CDS             complement(21692..22201)
                     /gene="yyaC"
                     /locus_tag="KR250_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore protease YyaC"
                     /protein_id="HBH0067275.1"
                     /translation="MYLAKVNYSDDDVIQMLSSVLKTIINENTIVVCIGTDRAIGDTL
                     GPLVGTILKNSNFKYPVYGTLDNPIHALNIYESLDTIKNTHIQGNFLAIDACLGSQSN
                     IGNIQIREGPILPGKGVGKKLPQIGNYSIVGIVDKIDENNKLSFNNIRLSFILDLAET
                     IALALLVST"
     regulatory      22390..22493
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            22629..23822
                     /locus_tag="KR250_001328"
     CDS             22629..23822
                     /locus_tag="KR250_001328"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HBH0067276.1"
                     /translation="MARHLFTSESVTEGHPDKICDQISDSILDALLEKDPQSRVACET
                     TVTTGLVLVAGEISTSAYVDIPKLVRETVREIGYTRAKYGFDCDTCAVITSIDEQSGD
                     IAMGVDEGLESKTGEEIEEEIEKVGAGDQGIMFGFACNETPELMPLPISLAHKLSRRL
                     TEVRKTGLVDYLRPDGKTQVTVEYEGSKAVRVHTVLISAQHCETVSNDKIREDLINHV
                     IKEVIPAELLDEDTKIYINPTGRFVIGGPQGDTGLTGRKIIIDTYGGYSRHGGGAFSG
                     KDPTKVDRSAAYAARYVAKNIVAAGLADKCEIELAYAIGIARPLSIFIDTFGTGKVSE
                     EKLVELVNKHFDLRPGAIIRDLDLRKPLYKKVAAYGHFGRTDIDLPWERTDKVEQLRK
                     DALGE"
     gene            24137..25234
                     /locus_tag="KR250_001329"
     CDS             24137..25234
                     /locus_tag="KR250_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0067277.1"
                     /translation="MSGKVNIKTVISFAGAYVATVIGSGFATGQEILQFFTFYGYAGI
                     IGGIISMVLFSWFGAEVIDKGRELKLKEPIKIYQVYCGKYLGTFFEWFGPLFLFGTLV
                     IMIAGAGATLSEYYGLNPYVGRIGMTIVSLITVSLGLTRLSKILGNIGPIIIIFTLLV
                     GAISLFSNIDALSNAGNMVDSLNIKTATSNGYFSGVLYTCYNVIIVITFLTGMGASAV
                     NKKDAVWGGIVGGVALMAAAIMMNLALLSDIGNIYTKEIPALYLADKISPIIGILFSV
                     VLLLGIYTTAVPLLWSVTNRFVEDDHPKFKIITIVVSILACIGGLLPFDKLVGTLYPY
                     TGYMGILILLCMLYRRITKTEGYKENKSEIS"
     gene            25498..27717
                     /locus_tag="KR250_001330"
     CDS             25498..27717
                     /locus_tag="KR250_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HBH0067278.1"
                     /translation="MEKLEGMISEIVFKNEDNGYTIAHLVNENDEIVVVGCMPTLAIG
                     ESIEVEGKWVNHKIYGTQFEVNSFMPVTPSSLEGIYVYLSSGMIHGIGEKMAKRIIDK
                     FGVDTLEVIQNSPEKLQEVEGIGSKKVKQIVKSYEEDRELRNIIIQLSPFGITPNYCL
                     KIYKKYKSSAIEVINKNPYQLAEDIRGIGFKVADSIANKIGIDKNSKDRICQGILYTL
                     NKSLSNGHTYLPEHVLIQDSEKLLELNGEIIKECVMMLVYNQKIHVEKVNNENLIYLM
                     PYYLAENGVCSQIVKLSQYEFEDLKIDIDNEINVLEEDKKIKLAEKQILAVKESVNSG
                     VLIITGGPGTGKTTTINAIIDIFENNGKSVTLAAPTGRAAKRMSETSNKEAKTIHRLL
                     EMGFSTDDDLTFFKDEEDPINSDVIIVDEVSMVDIILMYNLLRAIKLGTRVILVGDSD
                     QLPSVGAGNVLKDMINSNIINVVKLNEIFRQAQESMIIVNAHKINNGEPLYLNTKGKD
                     FFFIRKSTNEEILNEIIGLVNERLPKFYKVDKLKDIQVLSSMRKGELGVTNLNIELQK
                     YLNKKEKFKVEESFSKRLFRVGDKVMQVKNNYTKKWETEDQKESGEGIYNGDIGYVYH
                     IDKDKKTIYILFDQTKIVSYLYDELDEIDHSFCTTIHKSQGSEFPVVVLPIAWAPPML
                     LSRNLLYTAVTRAKKLVVLVGDVKYLEYMIKNNRVNQRYSNLGYKLNKFKQEGLLIE"
     gene            27720..28502
                     /locus_tag="KR250_001331"
     CDS             27720..28502
                     /locus_tag="KR250_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HBH0067279.1"
                     /translation="MKICNDNFIKNFKNMINKCLDFIYPENISCIICDKSIKKTNTYS
                     ICKSCFKEMNFIQDGCMKCGKPIIRHSIEKEFIEECSYCFNKDFYFDKSISCIEYNDV
                     TKKIVLGLKYNQKTFMAKYIAQIMKEKLYLENIKFDYILFVPLHKKRLNKRGFNQAQK
                     IAFNLSKIVNIPLLDCISRKKYTRMLYKLNKKERKEELKNVFVVKDNVKLINNKNILL
                     IDDIFTTGLTTNEISKLLKLSGANKVFVLTLLTKANDNYIME"
     gene            28834..30747
                     /locus_tag="KR250_001332"
     CDS             28834..30747
                     /locus_tag="KR250_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBH0067280.1"
                     /translation="MICLNKRQENLLTTLLLKEEFINVNKIANDFECSERTIRNDCKY
                     IDEWLSTFCNAYIKRKPNIGIKFSGDGTDKILVNQKLRGEVKRVNSELHKQVEILNLL
                     LCNNKKVTLNDLSNKLYINKNIVREEISKLSVALNNYNLNVSTKKGSGIYIGGDEKDI
                     RIMLVSFLFKYIDKYRLNIDEIEYFHIVDIIIVKNIMFTIEEDMDIRLTDISFKQLML
                     FLLINIIRIRLGNSLKVSNTKKELKLKMLILDIEDELKSNLSIVLNYEEKLFIESLIL
                     GMNKQINSSNIKKSLYLNEELASYTKKIINLVSEESGINFNNDILLYEQLICHLNVTM
                     HQMKSNVYLENPLLDNIKTKFCFLFTVISSSIEIELKGKQITEDEIGYLTLHFQTSLE
                     RKYSKRESNKKASIVCPFSFGVSMLLKVKIEKRFDNIKIIETLREEDLKRDNFNKTID
                     FIIAFQEYENIQKPIFITTPLFTDEDENKLKEFTNKIKEKDTSYKLMNRLMMSDYLIR
                     ELEHDDIYEAITYLTNLLIEKHYAEKEYLQSIITREKSYPTNVGKGILFPHGDMKYIK
                     QSVLCFARLKTPIRIRNGKDIKIILLLAYKKDDDRKVFRELFKEISNLTEDENMLDIL
                     SKCDIEKVKDILI"
     gene            30888..31196
                     /locus_tag="KR250_001333"
     CDS             30888..31196
                     /locus_tag="KR250_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBH0067281.1"
                     /translation="MEEIVLKIIIHAGNAKSMLYEALDYAKENDFKKADELIENANEE
                     ILKAHKVQTELIQKEAGGDKSDISILLIHSQDHLMTCMSERNLIKEMIMLRKEIQKLK
                     "
     gene            31220..31546
                     /locus_tag="KR250_001334"
     CDS             31220..31546
                     /locus_tag="KR250_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBH0067282.1"
                     /translation="MKRKVYLFCSFGMSTSLLADKMQKVADEHNLPIEVEAFPIAEID
                     KIVEEKNPDCILLGPQVKYMLKELKPKFEAQGKLIDIINEVDYGTMNGEKVLKLAIKL
                     IKSKQA"
     gene            31585..32919
                     /gene="celB"
                     /locus_tag="KR250_001335"
     CDS             31585..32919
                     /gene="celB"
                     /locus_tag="KR250_001335"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HBH0067283.1"
                     /translation="MNRLERILMPLAEKIGKNKLLIAIRDGFLISSPLLIIGSLFLLV
                     ANFPIKGWNEFVGQIFGSDWAVKLQQPTVATFEVMTLLAVLGIGYSYAKQIDVDPIAS
                     AAVAIVAFFIITPFVIPYTPEGTESVYMVTGIPLGWMGSKGMFVGMITAITSVKLFAA
                     VVKKGWTLKMPDGVPPTVSKSFAALIPSAIVMVVFFLVKIAFEATPYGSIHEFIFKFL
                     QMPLLKLGNSLGAMAIAYIFLHLFWFFGINGSSVVGAVYNPILKILSAENLAAFQAGS
                     KIPNIITGQFQDMFATFGGAGSTLSLVIAMLLICKSKRIKSMGKLSILPGIFGINEPL
                     IFGLPIMLNPILLIPFAIVPTINIIIAYFCMSAGLVPLTNGVQLPWTTPIIFSGFLTT
                     GWQGAVLQVVLLILGIFMYIPFIKMLDKQYLREENATVEEEDDDISFDDLVL"
     gene            32930..33448
                     /locus_tag="KR250_001336"
     CDS             32930..33448
                     /locus_tag="KR250_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/betaine/sarcosine/D-proline family
                     reductase selenoprotein B"
                     /protein_id="HBH0067284.1"
                     /translation="MKKIVMILDQIQAGAGGKEKSNIPPAGKSSPLGPGIMMEQFLNE
                     SKVIATLFCGDEFFVNNEEEVTSKMIAMVKKLNPDVVICGPSFNYENFSKMSAILSKN
                     INDKTDIPAFAAMSEENIDVINEYKNDICIVKTPKKGGIGLNDSLNNICKLAKAIANK
                     EDITLMKEEFCY"
     gene            33544..34503
                     /locus_tag="KR250_001337"
     CDS             33544..34503
                     /locus_tag="KR250_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase"
                     /protein_id="HBH0067285.1"
                     /translation="MWGMIATWRMALEGITEASEHLEKGMEAGDAVELAVRRVEDYPF
                     YKSVGYGGLPNENCEVELDAAFMDGNTLDIGAVAGIRDYSNPVSIARKLSYEKVNNFL
                     VGIGAEDYAHKNGFERKNMLTDRAKLHYKKRKKETLDKGLSPYAGHDTVGMISLDKTG
                     KMCAATSTSGLFMKKRGRIGDSALSGSGFYVDSSIGGATATGLGEDLMKGCISYEIVR
                     LMKEGKHPQEACDIAVNELDKQLIKRRGKAGDLSLVAMNNKGEWGVATNIADFTFSVV
                     TKEESPTVYISNRVDGKTIHKVISNEWMEAYEARIKAPIEVSE"
     gene            34552..35604
                     /locus_tag="KR250_001338"
     CDS             34552..35604
                     /locus_tag="KR250_001338"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HBH0067286.1"
                     /translation="MNIISNKIDELKEDLLSDIIDIVKIPSVKGESENGFPFGEKVGE
                     ALNKALEISEKLGFKVRNLDNYIGYAEHGDSDDYVCVIGHVDVVHEGDGWKHQPYKGE
                     ETNGRIYGRGVLDNKGPIMSALYGLYAIKELNLKLDKRVRIIFGTNEESGFEDIPYYL
                     EKEKAPIMGFTPDCKYPVVYGEKGMAKIRIKSKINYEEDVYLGFIENMSENVLVTYKE
                     LNIENSDTILDIKVKYDFSYKLKDVLDEIKASFPNSIDIEVISNFNPVYFDKESNLVK
                     KLQLAYERVTSLDGTPVTTNGGTYAKVMPNIVPFGPSFPGQKGIAHNPDEYMDIEDII
                     LNAKIFANAIYELAKE"
     gene            35607..36281
                     /locus_tag="KR250_001339"
     CDS             35607..36281
                     /locus_tag="KR250_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HBH0067287.1"
                     /translation="MLEIIGMSVEDAKIIEDCGADRIELVSALTEGGLTPSFGLIESV
                     VNSVKIPVNVMIRHHAKSFVYSKEDISIMQKDISVVKEIGANGVVFGVLDKNNSIDEK
                     NLNVLLKCCDNLDVTFHRAIDESNTIDSVKILKDYDKITNILTSGGKGSIVDNIQMIK
                     NMMLSSNHIKILLGGGLNFNNIEKIKELTKASNFHFGTAIRINNSPFEDIDRQKLKQL
                     VNIISR"
     gene            36834..37385
                     /gene="raiA"
                     /locus_tag="KR250_001340"
     CDS             36834..37385
                     /gene="raiA"
                     /locus_tag="KR250_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HBH0067288.1"
                     /translation="MNIIISGKQIELTDAIKNKIETKLSKLDNYIHPDTDVKVTVSAR
                     KARQKIEVTIATVNGPIIRAEDMQENLYTAIDVVYDKLSKQLKKYKKRLQDKHKDNKS
                     IRFQDAEINLADEQEDYGDENQELVIQRRKKFSVKPMSEEEAVLQMELIGHDFYMFKN
                     IDSDEIAVVYKRNNGGYGIIEHE"
     gene            37517..40192
                     /gene="secA"
                     /locus_tag="KR250_001341"
     CDS             37517..40192
                     /gene="secA"
                     /locus_tag="KR250_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HBH0067289.1"
                     /translation="MSFMDNLFNMADKKELKKFNKTVDIIDSLEPKFESMADSELKNM
                     TNIFKERLANGESIDDILPEAFAVVREVSKRVLGLRHYRVQMIGGIVLHQGRIAEMKT
                     GEGKTLVATAPVYLNALTGKGVHVVTVNDYLAKRDRDQMAKIYEFLGMSVGVIIHGQN
                     PKVRKEQYDCDITYGTNNEYGFDYLKDNMVIHKEQRVQRGLNYAIVDEVDSILIDEAR
                     TPLIISGPGDKSTHLYSDANTFVLTLKPDDYELEEKDKAVSLTASGIQKAEVYFNVDN
                     ITDISHTELYHHINQALRAHVIMKKDVDYVAKDGEIVIVDEFTGRLMFGRRYSEGLHQ
                     AIEAKEGLKIQRESKTLATVTFQNYFRMYKKLSGMTGTAKTEEEEFKAIYKMDVFQVP
                     TNKLMIREDLPDCVYKSEIGKFNAVAQEIIERHKVNQPILVGTVSIEKSELLSQILKK
                     KGIKHEVLNAKHHDKEAEIIAQAGRLGAVTIATNMAGRGTDIVLGGNPDFLTKREMRR
                     NGFKEEIVNRVDTPIEGIPVKGNEILFEAREEYEKLFEKFKQQTQEEQKQVVEAGGLA
                     IIGTERHESRRIDNQLRGRAGRQGDPGSSRFYIGLDDDLMRLFGSDRISGIVDKIGLE
                     EDMPIEHRILSKSIEGAQKKVEGKNFGIRKHVLQYDDVMNKQREIIYAERKRVLEGED
                     LQEQIQSMTHSIIEEAVTLYTQDKGFDEEGFKEHMYNLFLPKGSIEIPEIEKLNPVEI
                     TEKVYEIAMKIYTSKEEQVGYERMREVERVILLQAVDNHWIDHIDAMDQLRQGIGLRA
                     VGQQDPVIAYKMEGFDMFDEMNKHIKEDTVRYLFNITIETPVERKAVVDVENLSSPSD
                     GTLPTSKTVKKDEKVGRNDLCPCGSGKKYKNCCGR"
     gene            40207..41314
                     /gene="prfB"
                     /locus_tag="KR250_001342"
     CDS             join(40207..40281,40283..41314)
                     /gene="prfB"
                     /locus_tag="KR250_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_153309863.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HBH0067290.1"
                     /translation="MLKLQEYRHSMEQMASNLEELRVSLDIASLMKNIQINEEKMNKQ
                     DFWNDNEVAQRVLQENKSLKETLEEYESLKSLLEDIEVLIEIGLEEDDDSVERDIEKS
                     IESMEEKLSEMKIKTLLNGEYDKNNAILSINAGTGGLDAQDWAQMLLRMYIRWSESKG
                     YKVKLLDIISDPEAGIKTATILVEGTNAYGYLKSEKGVHRLVRISPFDPSGKRHTSFA
                     SIDVTPELDENIEVEINPSDLKIDTYRASGAGGQHVNTTDSAVRITHIPTGVVVQCQN
                     ERSQHLNKDRAMRLLMAKLIELKELEQKEKIEDIQGKYSQITWGSQIRSYVFQPYKLV
                     KDHRTNAEFGNVDSVMNGNIDLFINEYLKMNKIV"
     gene            41458..43599
                     /locus_tag="KR250_001343"
     CDS             41458..43599
                     /locus_tag="KR250_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HBH0067291.1"
                     /translation="MDINQILKKEFNLRDEQINNTLKLIDEGNTIPFIARYRKEMTGE
                     MSDVTLREFYEKLMYLRNLQSRKDDVVRLIDEQGKLTDEITQNIEKAKTLQEVEDIYA
                     PYKQKKRTRATIAKEKGLEKLALSILENNLDNIEIEAKNYLDEEKEVLSIEDALKGAR
                     DIIAELVSDDAKIRKYIRELALREGMIVSKSATDEKSVYDMYYDYSEAVKSMPPHRVL
                     AINRGEKENFLKVKLEINNDKVLNYIINEYVNDKNFKNKEEIVSSIEDSYKRLIFPSI
                     EREIRNHLTEIAQERAISVFGKNVKSLLLQPPVKDKVVMGFDPAFRTGCKIAVVDKNG
                     KLLDYTTVYPTDPQNDVEGAKKVLKGLIEKYDIDIISIGNGTASRESETFVSEMIKEI
                     DNEVQYVIVSEAGASVYSASELANEEHPDINVSIRGAISIARRLQDPLAELVKIDPKS
                     IGVGQYQHDLNKKRLEEVLDGVVEDSVNSVGVDLNTASYSLLEHVAGISKTIAKNIIA
                     YREENGDFTSRAQLKKVKRLGPQAFTQCAGFMRILEGKNPLDNTGVHPESYDICKKMI
                     EIIGYSLDDVKNKNICEIDEKIKEIGLRELSEKLEVGQVTLKDIITEIKKPGRDPREE
                     GIKPILRTDVLKIEDIQEGMTLKGTIRNVVDFGAFVDIGIKNDGLVHKSEMSNSFVKD
                     PMSIVTVGDIVDVKVIGIDLNKKRVALSMKK"
     gene            43747..44913
                     /locus_tag="KR250_001344"
     CDS             43747..44913
                     /locus_tag="KR250_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBH0067292.1"
                     /translation="MIFIKNGKINTITNGIIHGDILIDEGKIIEIGEDLIAPLDVEVI
                     DASNKLVFPGFIDAHTHLGLWEDGIGFEGADGNEETDPITPQLNPIDGINPMDRTFKE
                     AFEGGITSVCTTPGSANVMGGQCIAIKTCGKRIDKMVIKNPVASKIAFGENPKSCYGQ
                     DDKSPQTRMAIAALLRENLKKAEEYLEDIDMYESHDDEDCEKPEYDIKMESLIPVLRR
                     EIPFKAHAHRADDMFTAIRIAKEFNLKLTLDHCTEGHLIVDELVEEGFPVIVGPSLSE
                     RSKFELRNLTFNTAGILSNAGLDVCIMTDHPVIPVQYLPICAGIAVKHGMKEEKAIES
                     ITINPAKTLGIEDRVGSIEVGKDADLVIWDNSPLEIQSNVLYTIINGKVVYEKK"
     regulatory      44973..45090
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            45214..45819
                     /locus_tag="KR250_001345"
     CDS             45214..45819
                     /locus_tag="KR250_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBH0067293.1"
                     /translation="MQNTAKRGNMISTRTLVVMSILSAISYLLMFIQAPIPGIFPDFL
                     KIDLSDIPAIFGGMSLGPFVGFGIVVVKNVLQAITATTTGGIGEFANILIGGSYVMIL
                     CYSYKRSGDLKGVLIGFVLGTISMTIIGSLVNYFVMLPLYGQLMGLDAIIGLGSAINP
                     QVHDLFTFVIWMIAPFNVLKAVILSLVTLPLYKKMGNVIKK"
     gene            46148..47188
                     /locus_tag="KR250_001346"
     CDS             46148..47188
                     /locus_tag="KR250_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HBH0067294.1"
                     /translation="MNLSKKIKKLFLDDKGYYFNSNWKDIHIGIYNTKLGDSLKYITN
                     EYIENLERVKYDYRELKNIENQTDGMCILAIRQNYKALEYVKNQTEELCLEAIKYNYK
                     ALEYVKEQTEYICLEAIKKDCNALKYVRNKTERLIIKVIRHSSNIDVVSILKALETQT
                     RRICLEAIKKDGRCLAYVREQSEELCIEAIKQNYKALKYVENQTEKMCIESVRQNGMA
                     LQYVNKQTDKICIEAVKQDGRSLQFVNNKTEEICINAIRYLNKKYNIKDVLSYIDKYT
                     EDICIEIVRQNGKMLMYIKNQTEKMCIEAVKENYKSLKYVKEQSERICKEALKQNHKA
                     KEYVKIAIDDCI"
     gene            47327..47779
                     /gene="tsaE"
                     /locus_tag="KR250_001347"
     CDS             47327..47779
                     /gene="tsaE"
                     /locus_tag="KR250_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HBH0067295.1"
                     /translation="MAKIYLENENKTREIGYKLGKLLKEGSVICLVGDLGAGKTTMTQ
                     SLADSLGIEDYITSPTFTIINEYEGKIPLYHFDVYRIGSSDEMYDIGYDEYVNSNGIC
                     IIEWANLIEDILPKEYLNIELRYKDDGREMILTPKGEFYKEIVEELIK"
     gene            47779..48495
                     /gene="tsaB"
                     /locus_tag="KR250_001348"
     CDS             47779..48495
                     /gene="tsaB"
                     /locus_tag="KR250_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HBH0067296.1"
                     /translation="MKILGMDTSSMAASVAVVEDDNLICEFTVNNKKTHSQKLMPMIE
                     NMLSMSDLSIKDMDLLAVCIGPGSFTGLRIGMATVKAMAHVNNIPIIAVNSLESLANN
                     INFCDRKICCILDAQKNQVYSAKYKFENGEMIELDGVDVVEFDALVDEIVSTNEEFII
                     VGEAVYKYKDKLENIKNIKIPSPANNVSNAGSLCSLALNKYNKNIDVHTCYTINPMYI
                     RKSQAEVQYDEKMKRLNNGR"
     gene            48485..48961
                     /gene="rimI"
                     /locus_tag="KR250_001349"
     CDS             48485..48961
                     /gene="rimI"
                     /locus_tag="KR250_001349"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HBH0067297.1"
                     /translation="MEDNTKLIVDDIKIEEMTTEDIDEVFEVEKNCFEDYWSKESFRK
                     ELSNEVARYIVAKLNGKIVGYVGIWLILDEGHINNVAVHSDYRGKKIGDKLIKGIVDL
                     CKDNNIASMTLEVRASNKIAQNLYRKYGFKMGGIRKEYYSDNKEDAIIMWNQLKEV"
     gene            48967..49983
                     /gene="tsaD"
                     /locus_tag="KR250_001350"
     CDS             48967..49983
                     /gene="tsaD"
                     /locus_tag="KR250_001350"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HBH0067298.1"
                     /translation="MSDIITLAIESSCDETAASVLKNGREVLSNIISTQIETHKKFGG
                     VVPEVASRKHVENIDIVVQEALDKANIGFNDIDHIAVTYGPGLVGALLVGLSYAKALA
                     YTLNIPLVGVNHIEGHLSANYIEHKDLKPPFITLIVSGGHTHLVEVKDYGKYEILGKT
                     RDDASGEAFDKISRAMNLGYPGGPIIDNLAKKGNKHAIEFPRAYLEEDSYDFSFSGLK
                     SSVLNYLNGKRMKNEEIVVEDVAASFQEAVVEVLSTKALKAVKEKGYNIITLSGGVAS
                     NSGLRAKITELAKDSGITVKYPPLILCTDNAAMIGCAGYYNFINGKTHDMSLNAVPNL
                     KINQ"
     gene            50280..52988
                     /gene="hpdB"
                     /locus_tag="KR250_001351"
     CDS             50280..52988
                     /gene="hpdB"
                     /locus_tag="KR250_001351"
                     /EC_number="4.1.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase large
                     subunit"
                     /protein_id="HBH0067299.1"
                     /translation="MSQSKEDKIRSILEAKNIKSNFQNKENLSEFNEKKASKRAEDLL
                     DVYYNTLSTADMEFPYWYNREYRKSDGDIPVVRRAKALKSAFSHMTPNIIPGEKIVMQ
                     KTRHYRGSFPMPWVSESFFVAQGEQMREEAKKLASNTADELTKFGSGGGNVTESFGNV
                     VSIAGKFGMRKEEVPVLVKMAKEWVGKSVEDLGFHYEKMMPDYDLKENLMSTLICMFD
                     SGYTLPQGREVINYFYPLNYGLDGIIEMAKECKKAVAGNASGDGLIGMDRLYFYEAVI
                     QVIEGLQTWILNYAKHAKYLESIETDLEAKKEYSDLVEILEHIAHKQPRTFREALQLT
                     YTIHIASVNEDAISGMSIGRFGQILYPWYEQDIEKGLITKEEVIELLELYRIKITCID
                     CFASAGVNGGVLSGNTFNTLSIGGLKEDGSTGANELEELLLEASMRCRTPQPSLTMLY
                     DEKLPEDFLMKAAECTKLGSGYPAWVNNSNGTTFMMKQFADEGMTVEEARAFALGGCL
                     ETSPGCWKQLTLNGKTYSIAGGAGQSAGSGVHFIANPKILELVLMNGKDHRMNIQVFE
                     PHNKPLDTYEEVIEVFKDYYKQAINVLERANNIELDIWRKFDTSIINSLLKPDCLDKG
                     QHIGNMGYRYNATLNVETCGTVTMVNSFAALKKLVYDDKAFTIEEMKDAILNNFGFKD
                     ALEVGNYSMADQVKVDKTGKYDAIYKACLDAPKYGNNDLYADNILKNYEVWLSKVCEE
                     AQSLYAKKMYPCQISVSTHGPQGAATLATADGRLSGTTYSDGSVSAYAGTDKNGVYAL
                     FESATIWDQAVVQNSQMNLKLHPTTIKGQQGTKKLLDLTRSYLRKGGFHIQYNVVDSE
                     TLKDAQKNPDNYRQLMVRVAGFTQYWCELGKPIQDEVIARTEYEGV"
     gene            52992..53249
                     /gene="hpdC"
                     /locus_tag="KR250_001352"
     CDS             52992..53249
                     /gene="hpdC"
                     /locus_tag="KR250_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase small
                     subunit"
                     /protein_id="HBH0067300.1"
                     /translation="MRKHSDCMNFCAVDATKGICRLSKQMINLDDAACPEIKVMPKCK
                     NCKNFVEANDEGIGKCVGLEKEDWVYSTLNAITCEGHVFNE"
     gene            53242..54192
                     /gene="hpdA"
                     /locus_tag="KR250_001353"
     CDS             53242..54192
                     /gene="hpdA"
                     /locus_tag="KR250_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase activase"
                     /protein_id="HBH0067301.1"
                     /translation="MSSQKQLEGMIFDVQSFSVHDGPGCRTTVFLNGCPLSCKWCANP
                     ESWTVRPHMMFSELSCQYENGCTVCHGKCKNGALSFNLDNKPVIDWNICKDCESFECV
                     NSCYYNAFKLCAKPYTVDELVQVIKRDSNNWRSNGGVTFSGGEPLLQHEFLHEVLLKC
                     HEVNIHTAIETSACVSNEVFNKIFNDIDFAFIDIKHMDREKHKEQTGVYNDLILENIS
                     NLANSDWNGRLVLRVPVISGFNDSDENISDIISFMHKNNLVEINLLPFHRLGESKWIQ
                     LGKEYEYSDKGDVDEGHLGELQDIFLDNGIACYVGHETAF"
     gene            54507..55469
                     /locus_tag="KR250_001354"
                     /pseudo
     CDS             54507..55469
                     /locus_tag="KR250_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112760.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
     gene            complement(55568..>56220)
                     /locus_tag="KR250_001355"
     CDS             complement(55568..>56220)
                     /locus_tag="KR250_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBH0067302.1"
                     /translation="NEFIIINLILTFISSLSIIIYSSNKKNLYPIKNIKLGSLLIIYF
                     MISVIVPTSFILILQGSQPNYSGIIKFIFTPITSLTTIYIFFSEEYAWRGFLQNIFFN
                     KFGKKLGVIILGICWSLWHLPLIFTLYTPETPILGLILRSIHIVGISIFLGYLYIKTK
                     NIWFCAIIHILNNSAFLITNSKESTITYHDILIVSMVVLIFYVPFLFTKEYKNNLE"
BASE COUNT        22484 a         6110 c        10704 g        16922 t
ORIGIN      
        1 ctgccaaggg catggctggg tagctatgta cggaatggat aagcgctgaa agcatctaag
       61 cgcgaagcca acttcaagat aagatttccc accgcaaggg taagacccca gaaagactat
      121 ctggttgata ggtcgaaggt gtaagtgcag caatgtattt agcttatcga tactaatagg
      181 tcgaggactt gaccaatatt tatttgatgt tcattcatta agatatatgt gtagttttta
      241 gagtgctaac tctaaagaaa ctagtattgc taggttctat aaacttatta aaaacttaat
      301 agaaatatta agcataaaga ttatgtggtt acaatagcag agaggataca cctgttccca
      361 tttcgaacac agaagttaag ctccctagcg ctgatggtac ttggtgggaa actgcctggg
      421 agagtaggac gtagccacgt taaatgttcc aaggtagctc aacggtggag caaccggctg
      481 ttaaccggta ggctgtgggt tcgagcccca cccttggagc caaaaaagcc gaagtggcgg
      541 aactggcaga cgcacaggac ttaaaatcct gcgggactta cctctcgtac cggttcgatt
      601 ccggtcttcg gcacttataa aacaatatcg cggggtggag cagttggcag ctcgtcgggc
      661 tcataacccg aaggtcgtag gttcaagtcc tgcctccgca accattaaaa cggctcgttg
      721 gtcaagaggt taagacaccg ccctttcacg gcggtaacag gggttcaatt cccctacgag
      781 tcaccaattc aattaaatat gcgggtgtag ctcaatggta gagttctggc cttccaagcc
      841 agctgtgagg gttcgatccc cttcacccgc tccaaaaatg ggaccatagc tcagctggga
      901 gagcacctgc cttacaagca gggggtcaca ggttcgagcc ctgttggtcc caccattcgc
      961 ggcctggtag ttcagttggt tagaatgcca gcctgtcacg ctggaggtcg agggttcgag
     1021 ccccttccag gtcgccaaat taaataagcg ggaatagttc agtggtagag cgcaaccttg
     1081 ccaaggttga agtcgcgagt tcgaatctcg tttcccgctc cataataact tgggtgcata
     1141 gctcagctgg atagagcaac gcccttctaa ggcgtgtgtc cggggttcga atccctgtgc
     1201 gctcattaag aaaagtttac tataaattat agtaaacttt tatattgttt aaaaagacac
     1261 aggaaacatg tatattacct gtgtcttttt atttataaac tttaaactat gtaaaaatta
     1321 cagtatattc taatagagtt acagtatatt cattaatatt gtaaacatac ggagggtaat
     1381 gtataatagt ctacaagatg tttatgaagg catcagttaa atataataaa tataactaat
     1441 tagtaagagt tatatacatt atattgtata ctttatggta tttatatatt gaaataactt
     1501 atgaaattat ttataaagag attcatgagc atatttttgt ctaaacaata agtataagaa
     1561 attataaaaa gttagagact atagtgaatt tacaatttaa tgtaatattt ataagttata
     1621 ctaaataaat tattatactg tgaaatggat atataacttt aggtattgtg tattattttc
     1681 actgtaggag attatatcta gttatacata ataagtttta atgatagttt atagtaagat
     1741 attaaaataa atttatctaa tctagatatg aaataagtta ttactttaat ataaaaatga
     1801 atctaatgga ataatattga tgataagtaa tatttagatt ttgtatttgt ttatagttaa
     1861 taagtggatt atagaattta taataattaa tatatattaa acaaagatat attaagatat
     1921 tttagaacat aaaactgaat taaggcgcga gtactatttt gtattcgcgc cttgatggtt
     1981 tgagttattt gagtaaaaac taactttaat aagaaatatg tatttattaa gttgatattt
     2041 ttaatgtgtt tagagattat ttaacaatct ccaaatataa aactagaatt atttacataa
     2101 aagttaaata gtttgtatag agttagattt atatagtata atacttagtg taaataatta
     2161 agaagttatc aaacaggagg ataaaactgt gttagatata aattcttttt taaatggatt
     2221 ttttaatata atattaagaa tgagtatata tgatttaatt gacatatcta tagtagcgta
     2281 tattttttac aaaatattta tgtttataaa agatacaaga gcagaacaag tgtttaaagg
     2341 tataatattt cttttattag caactcaact tagtaacacc tttaaattgc ataccgttta
     2401 ttggatatct cttaaagctt tagattatgg agttatagct gctcttataa tttttcaacc
     2461 agaatttaga gcaggacttg aacatatagg aagagcaaag tttaatttat ttggaaagaa
     2521 tgtaaatact tctgaagaaa ctctcaatag aaatatagaa gaaattgtag aagctttata
     2581 ttcactttct agacaaaaaa taggagctct tatcataatg gagagagaaa ctagaataag
     2641 tgatataata aatacaggaa ctattattga tgctgaaatt tctaggcaac tattaataaa
     2701 tatatttata ccaaatacac cattacacga tggagctgtt gtaattagag attctaaggt
     2761 aaaagcagct gcatgttttt tgccattgac agaaagtaag gatttaagta aagatttggg
     2821 cactagacat agagcaggaa taggggtaag tgaggtgtct gactgtataa ctttgattgt
     2881 atcagaggaa acaggtggcg tatcaattgc taaagcaggt aagttatata gagatatttc
     2941 tagagaaaga atgatgaata tattacgtag taatttaaaa acaaatacag aaacaagaag
     3001 ttttttcaaa ggtggtatat ttaaatgata aatagactaa aaaataatac caaaataaaa
     3061 ataatatcat ttttaagtgc aattgcatta tggatgtatg ttatggctgt agttgaccct
     3121 gaagaaacta aagggtttga agatatacct gtatctataa gtaatatgag tgatttaaag
     3181 gataaaaatc ttgttatata tccagacgaa aaattaacag cagatgtata tataaaaggt
     3241 agtttatcaa aggtacaaag tgttaaaaaa gaagatatac atgtatatgg aaccattgaa
     3301 gacccaatac aaggacagaa ggaagtatat ttaaaagcaa atattcctca aggtgttact
     3361 ctagaattta agcatgatgt tttagtagta aatcttgaaa aaaacataaa agaaaaaaga
     3421 gatataaagg tagaagtaag tggaaattca aaaagaaata taaataaaat tgaacttagt
     3481 aaggaatttg tcgatgtatc aggtccaaga actctagtta aagaagtcaa aagtgtaaga
     3541 gctaatctag atgttggtaa tgagacagat gatttttcta aaaaattgca attaatacca
     3601 ttaaatagtg atggaactaa ggttgaagga gtaacattaa gttcatcctt tgtgactgca
     3661 aagatagagc tattaaagga aaaaattgtc ccaataagag taagtctaaa tgataataaa
     3721 gataatgatg tactgaaaaa ctataaatta agtaaggaac aagttacaat aaaaggaaag
     3781 aaagaagata tagacaaaat aagttatatt aagacaaaac ctattgactt agctgatttg
     3841 gctaatggtg aagaaaaaga agttagctta gaaattccag agggtataac tcttgaggat
     3901 agctctataa caattaaatt aaatgataca aaacaattga gtgcagaatt tttatataat
     3961 gcgggagata ttgaattaag aaatatccca acaaatatgg ataatccaaa tgtaatttct
     4021 gatgtagata taaaagttgt tgtagagtca acggaggatt tgagtaagtt aaataaaaaa
     4081 gatatttcat tatatattga tttatcacaa ggtcctgagg aagatagtaa atataaaata
     4141 aaatatgaaa caacatatca atttaaaaaa ataaatatag aacctaatat tgttgaagtg
     4201 gaatgaacaa atatttcgaa tatttagttg ttacaagatg cgacaaaaaa acctataaaa
     4261 ttaagtttgt taattttatg tttttgttgc atcttgttta ttttagttat attataaaat
     4321 aaggacaatg agatatttgt taaatctaaa aagcgctaga tatatgaaaa aagactgata
     4381 aatagattta aataaatatt ttgaattaaa tatttcaatt atctctagga aaacgttgct
     4441 gaacgagtta taatttaatt gagatataaa aagttctatt tcaaaaaatt caagatgagg
     4501 tggaaatgca tgagaagttt tgaagaagta attaagtttg caaaagaaag aggacctaaa
     4561 actatatcag tagcatgttg ccaagataaa gaagttttaa tggcagttga aatggctaga
     4621 aaagaaaaaa tagcaaatgc cattttagta ggagatatag aaaagactaa agaaattgca
     4681 aaaagcatag acatggatat cgaaaattat gaactgatag atataaaaga tttagcagaa
     4741 gcatctctaa aatctgttga attagtttca caaggaaaag ccgacatggt aatgaaaggc
     4801 ttagtagaca catcaataat actaaaagca gttttaaata aagaagtagg tcttagaact
     4861 ggaaatgtat taagtcacgt agcagtattt gatgtagagg gatatgatag attatttttc
     4921 gtaactgacg cagctatgaa cttagctcct gatacaaata ctaaaaagca aatcatagaa
     4981 aatgcttgca cagtagcaca ttcattagat ataagtgaac caaaagttgc tgcaatatgc
     5041 gcaaaagaaa aagtaaatcc aaaaatgaaa gatacagttg aagctaaaga actagaagaa
     5101 atgtatgaaa gaggagaaat caaaggttgt atggttggtg gaccttttgc aattgataat
     5161 gcagtatctt tagaagcagc taaacataaa ggtataaatc atcctgtagc aggaagagct
     5221 gatatattat tagccccaga tattgaaggt ggtaacatat tatataaagc tttggtattc
     5281 ttctcaaaat caaaaaatgc aggagttata gttggggcta aagcaccaat aatattaact
     5341 tctagagcag acagtgaaga aactaaacta aactcaatag ctttaggcgt tttaatggca
     5401 gcaaaggcat aattttaagg gggaaatagg caaatgagca aaatatttaa aatcttaaca
     5461 ataaatcctg gttcgacatc aactaaaata gctgtatttg ataatgagga tttagtattt
     5521 gaaaaaactt taagacattc ttcagaagaa ataggaaaat atgagaaggt gtctgaccaa
     5581 tttgaatttc gtaaacaagt aatagaagaa gctctaaaag aaggtggagt aaaaacatct
     5641 gaattagatg ctgtagtagg tagaggagga cttcttaaac ctataaaagg tggtacttat
     5701 tcagtaagtg ctgctatgat tgaagattta aaagtgggag ttttaggaga acacgcttca
     5761 aacctaggtg gaataatagc aaaacaaata ggtgaagaag taaatgttcc ttcatacata
     5821 gtagaccctg ttgttgtaga tgaattagaa gatgttgcta gaatttctgg tatgcctgaa
     5881 ataagtagag caagtgtagt acatgcttta aatcaaaagg caatagcaag aagatatgct
     5941 agagaaataa acaagaaata tgaagatata aatcttatag ttgcacacat gggtggagga
     6001 gtttctgttg gagctcataa aaatggtaaa atagtagatg ttgcaaacgc attagatgga
     6061 gaaggacctt tctctccaga aagaagtggt ggactaccag taggtgcatt agtaaaaatg
     6121 tgctttagtg gaaaatatac tcaagatgaa attaaaaaga aaataaaagg taatggcgga
     6181 ttagttgcat acttaaacac taatgatgct agagaagttg aagaaagaat tgaagctggt
     6241 gatgaaaaag ctaaattagt atatgaagct atggcatatc aaatctctaa agaaatagga
     6301 gctagtgctg cagttcttaa gggagatgta aaagcaatat tattaactgg tggaatcgca
     6361 tattcaaaaa tgtttacaga aatgattgca gatagagtta aatttatagc agatgtaaaa
     6421 gtttatccag gtgaagatga aatgattgca ttagctcaag gtggacttag agttttaact
     6481 ggtgaagaag aggctcaagt ttatgataac taatgataaa agaataagaa tcataactgg
     6541 tcactatgga agtggtaaaa gtgaatttgc gatgaactat gttgttaagt taagagatat
     6601 ggtttctggc aaagttgcta tagccgattt agatgtggta aatgtatact ttagaactag
     6661 agaaaagaaa gagttaatga aatccttagg tatacaacca atagatagct caattaatgc
     6721 tccaacatta gatttaccag cagtatcagc agaggtaatg tctcctatgg tagaccattc
     6781 atacaattct gttatagatt taggtggaga taatgtaggt gctagggtta taggaagatt
     6841 tagtcatttg ttaaaagaag gcgattatga tatgcttttt gtaataaatg caaataggga
     6901 gaaaactcaa acttctgaag aagttatcca atatataaaa gaaatagaaa aatcttctaa
     6961 attaaaggta acaggtctaa ttaataatac tcatcttatt aggtttacta ctattgatga
     7021 tgttttaaga ggtcagaaag ttgcaaaaga agtttctgaa aaatgtaata taccgataag
     7081 atatgttgca tgtttagaaa atcttgttga acagttacca aaagatttag aaggagaaat
     7141 ttttcctata aaattatata tgagagaaga ctggatgtag tttgagctca aatatataga
     7201 aagtttaagg aggcttaggt aaatggctaa aggaaaagta tcttttaatc aagaacgttg
     7261 taaaggttgt ggattgtgtg ttgaagcatg tccagtaaaa atcatacaat tagactcaaa
     7321 tgtaataaac aaaaaagggt ataacccagc aactgttttt gaaatggaaa aatgcatagg
     7381 atgtgcaagt tgtgcaacta tgtgtccaga tgtagttata acagtagaaa gagattaatt
     7441 taattaatat ttaaggggga attttaatgg ctaagatact tatgaaaggt aacgaagcat
     7501 ttggaaaagc agctatagaa gctggttgca aatatttctt cggttaccca ataactccac
     7561 aaagtgaatt accagaatat ttatcaagag agttacctaa aataggtgga gcgttcgttc
     7621 aagctgaatc agaagtttct gcgataaaca tggtttatgg tggagcaggt gcaggagcaa
     7681 gagttatgac ttcttcatct tcaccaggag ttgctttaaa gcaagaaggt ataacatatg
     7741 cagtaggagc agaagttcct tgtgttgtac ttaatgtaat gagaggtggt ccaggacttg
     7801 gaagtataca accttcacaa gctgactact ttatgtctac aagaggtgga ggaaatggag
     7861 actacagaac tccagtattc gcaccagcta cagttcaaga agctgttgat atgataatgg
     7921 aagcttttga cgttgcagat tattacagat caccagttat ggtagttgca gatggtatga
     7981 taggtcaaat gatggagcca gttgaattta gagctcctga gaagaaaaga gaattgcctc
     8041 ctaaagattg ggcaactgtt ggaactaaag gaaaaagaaa acctaatgta ataaactctc
     8101 tatacttaga gccagaagta ctagaagacc attgttggca tttacaagaa aaatttgatg
     8161 caatggaaaa gaatgaagta caatacgaaa tgtacaaaac agaagatgct gaatttgtat
     8221 ttgcagctta tggaactact tcaagagtag taaaaagcgc aattgatata ttaagagaag
     8281 aaggaataaa agcaggtctt ataagaccta aagtattatg gccattccca ttcgaagcat
     8341 tcaaccaaat tcctaatgct agaaacatat tgactgttga aatgagtatg ggacaaatgg
     8401 tggaagatgt taaaatggca gtagaaggta aattaccagt ttacttccat ggaagaccag
     8461 gaggaatgac tccaacacca gcagaaatag ttgaaaaagc taaaaaaatt attgccggag
     8521 aattggttgc aggaggtgct agataatggc tgttgtattt aagaaaactg aaggattaca
     8581 agatactcaa actcactact gtccaggatg tacacatggt ataatccata gattagttgg
     8641 tgaagtatta gaagaactag gagtattagg tgatgctgta ggtgttgttc cagttggatg
     8701 ttcagtttta ggatataaat attttaactg tgacactcaa gaagctgctc atggtagagc
     8761 gccagctgct gctacaggaa taaaaagagt tcatcctgaa aatacagtat tcacttatca
     8821 aggtgatgga gacttagctt caattggtac tgctgaaata gttcatgcag ctgctagagg
     8881 agaaaaaata actactatat ttgtaaacaa tacaacttat ggtatgactg gtggacaaat
     8941 ggctccaact acattagttg gacaaagagc tactactgct caatcaggaa gaaatgctga
     9001 aactcaaggt tatccaataa gagtaagtga aatgttagct acattaacag gagctgtatt
     9061 tgtagaaaga gtagcagttg atactccagc tcatgttaga caagctaaaa aagctataaa
     9121 gaaagcattc caagttcaac aagcaggatt aggatttgga atagtagaag tattatctac
     9181 ttgtcctaca aactggggac tagctccaaa cgatgcttta caatggttaa gagacaacat
     9241 gataccatac tatccattag gaaatttcaa aaatgttgaa gttgaggagg tgaagtaaat
     9301 gtctacagct agagtaatat gtgcaggctt cggaggccaa ggtgttatgt ctatgggaca
     9361 attacttact tatgcaggaa tgctagaagg aaaagaagtt tcttggttac catcttatgg
     9421 accagaaatg cgtggaggta cagcaaactg tgcagtaact gtatctaatg agccagttgg
     9481 ttctccactt ataacagatg atgcaactgc agctatatta ttaaatatac cagcatttga
     9541 aaaattcaaa gatgatgtta ttcctggtgg aaaaataata gtaaatagtt ctttaataaa
     9601 agaaaaagtt gatagaacag atgtagatgt ttattatata ccagcaaatg aattagcagc
     9661 tgaattagga aatgataaag ttgctaacat gataatgtta ggtgcttact taaaagtatc
     9721 tgatactgta gatatagaat ctgtactaga agcattcaaa aaagttttcg gacctagaaa
     9781 agaaaaattc gttccattaa acagagaagc tttacaaaaa ggtatggatg ctacatgttc
     9841 taaagcaact aactaataaa gaaaaagatg tcaaaaaaaa gacaatagaa ttatttctat
     9901 tgtctttttt gctgataagt attaaaatat ttttatgact gaaattaagc ctataatgat
     9961 attttatgaa ctataaaaat ataatacaaa ttacaagctt ttcaattatg aaaggagaat
    10021 ctttagtgag aaagtatttt ggaacagatg gagttagagg agtagcaaat acagaactaa
    10081 catgtgattt agcatataaa cttggtagag ctggtggatt tgtcttagct caaggagacc
    10141 acagagtaaa agtagtagta ggtaaggata ctagaatatc aggtgatatg ttggaagcat
    10201 ctttaatagc aggacttatg tctgttggat gtgatgttat tactgttgga ataataccaa
    10261 cacctgcagt ggcatattta actagaaaat atggtgctga ttgtggtgtg gttatatctg
    10321 catctcacaa tcctgttgaa tataatggaa tcaaattctt taataagaat ggatataaat
    10381 tagatgatga aattgaattg aaaatagaag aatatataga cgatatagac aaaattgatt
    10441 gtcttccaat tggtgaaaat gtaggaagaa aattacatga acattgtgca caaagagact
    10501 atgtagacta cctaaaatca ataataagca ctgactttaa gggattaaaa gtagttttgg
    10561 actgtgctaa tggtgcgtca tataaagtgg caccaatagt gtttgatgaa ctaggagcaa
    10621 gtgtaatttc aataaatagt tcaccagatg gaaataatat aaactataaa tgtggctcta
    10681 cacatccaga acaacttcaa agggctgttt tggaacataa tgctgactta ggtcttgcat
    10741 atgatggaga tgctgataga ttaattgcag taaatgaaaa gggtcaaatt gttgatggag
    10801 accatataat gatattgagt gccttaaatt taaagaaaaa taataaattg gcacaagata
    10861 ctttggtagt tacagttatg agtaatatag ggttgacaat agctgctaag gaaaatggaa
    10921 taaatttatc tactacagca gttggagaca gatatgtttt agaagatatg gttaaaaatg
    10981 gatataatct tggtggagag caatctggac atatgatatt tttagattac aacactactg
    11041 gagatggagt gcttagttct ctaatacttg caaatataat cttgcaagaa aagaaacctt
    11101 tatcagaaat tgcatcaata atgagccaat acccacaagt attagtaaat gcaacaataa
    11161 aaaatgaaaa taagaacaaa tatatggaat atccagaaat aaaaactgag atagaaagaa
    11221 tagaatctat tttagacgga aatggtagag ttttgataag accatcagga actgagccat
    11281 tagttagagt tatgttagag ggtaaagagg aaggtcaaat aaaagaatta gcaacaaact
    11341 tagctaatct tatacaagaa aaattatcgt aatttaaagg gtattctcag atataaatac
    11401 tgaggacacc tttctaagcg ccagaactta gttatagaca taattattga gtaatatagg
    11461 aactaagttg acgaggtcag agttaatcga attgtcggcg gatgctctgc ggtgtgttta
    11521 ccatcgaaag acttctttaa ataataggag caatcctatg gacaaaggga agccaaagta
    11581 aacttatatg acctaactct taaaatatat aaataatatt tttaggagga cacaaataat
    11641 gtgtggaata gttggatatt taggaagtag aaaggcagca gaagttatag tagaagggct
    11701 ttctaagcta gagtacagag gttatgactc agcaggagta gcagttaata gttctaatga
    11761 aaaagaactt aatattagaa agtttaaagg tagactttct gtacttgcag aagacttaga
    11821 aaagaatcct attgatggaa atttaggtat agggcatacg agatgggcaa ctcatggaga
    11881 accatctgat gtaaactcac atcctcattt taatcaagcc aaaacaatag cagttgttca
    11941 taatgggata atagaaaact atatggaaat aaaagaagaa ttaatttctg aaggtgttaa
    12001 gtttgaatct caaacagata cagaggtaat agcacattta gtagataaat actatgaggg
    12061 taatttatta gatgctgtat acaaaactat atcaaaatta agaggagctt atgcattagg
    12121 tgttatatgt aaagaacatg gaaatgaatt agttgcagta agaaaagata gtcctcttgt
    12181 agttggtgtt ggtgaaggtg aaaactttat agcatcagat atacctgcat tattaaaata
    12241 tactagagat gtttatttct tagaaaatgg agaagtagtt catctaaaag acgaaaatgt
    12301 aacagtttat gactctaata gaaatcttgt tgaaaaagaa gtatttcatg taacttggga
    12361 tgtagaagct gcgtctaaag gtggatatga ctactttatg tcaaaagaaa ttcatgagca
    12421 accaacagga gtaagagaaa ctttagaaag aagacttgat gataatggaa atataattct
    12481 agacagtata aatatttcta aagaagactt agaaaaaata aataaagttt atatagtggc
    12541 ttgtggaaca gcatataatg ctggattact tggtaagtat gccatagaaa aatttgtaaa
    12601 tataccagta ataactgata tagcatcaga atttagatat agcgacccat ttgtagatga
    12661 aaattcatta gtaatattag ttagtcaatc tggtgagaca gcagatactc tagcagtatt
    12721 aagagattca aaggctaaag gagcaagaat actttcaata actaacgttg taggttcatc
    12781 tatagcaaga gaatcagatg atgtattcta tacttgggca ggacctgaag tagcagttgc
    12841 atcaactaag gcatatacta ctcaaataac ttctttatac atgatagctt tagactttgc
    12901 tatcaaaaaa ggaacaataa ctagagaatt ttatgatagt atgatttcta agatgaaaga
    12961 aataccatca aaaatacaag aaatactaga caatgaagaa tatataaaag aagttgctaa
    13021 aacagttgtt agttctgaac atgcattcta tttaggaaga ggtatagact atagcttagc
    13081 aatggaaggt tcattgaaat taaaagagat ttcatatata catgcagaag cttttgcagc
    13141 aggagagcta aagcatggaa caatagcttt aatagaaaaa ggaactcctg taattgccat
    13201 agctactcaa gaaaagttat ttgaaaaaat ggtttctaat atggaagaag taagagcaag
    13261 aggagcttat gtagtagcaa tagctcaaag ccataataag gatgttgaga aagctgctga
    13321 taaaataatc tatataccaa attcagatga tattttatca ccaatattag cagttgtacc
    13381 aatgcaatta cttgcttatc atgtctctgt actaagagga tgcgatgtag ataagccaag
    13441 aaatctagct aaatcagtaa cagttgaata aaaacagatt aaaaattaaa tagataatat
    13501 ttattgaaaa gaactcatcc tgttatttgt attttatagg atgagttttt taaataaata
    13561 ttaaatttgt tttgtttttg ctataataaa ttttataaaa agattgatat taaatcatct
    13621 gtaaaatgat aaatatatag accataccag gtatagggaa cgataaatat agttcatata
    13681 aaatatagtg agtacttaat aaacatatag tcaatatcag ataaaaaaaa taatagctta
    13741 tgagaaaaat taaaatacaa ggtattagta accttgtgat aagataaagt gagtagttta
    13801 aaataagaat ataaggaact atttataaaa gaatataatg aagctaataa agtataagaa
    13861 aattacttta ttttagtaaa ataatttcaa ataaaaattt tttaaattgg gagtataaat
    13921 tatgacaaat gattttaaaa aagaaaagaa agaagattat tttgttaatc ttaaggcaat
    13981 atctactgaa gttttacaag aaaaagtgca agataacgct tgggttatag ttgatactcg
    14041 cttaaatgat gcttataatg gctggaagtt agatggagta aaaagaggtg gacatataaa
    14101 aggagcagta gatttttctg ctaattggtt aagtgtatac tcagatagaa aagatgaagt
    14161 gctagaacaa gcattgaaaa ctaaaagaat tgatttagat aaaaatatag tattatatga
    14221 tgcaaatgga aaagatgctt tggttgtagc agattattta tctaaaaaag gatataaata
    14281 tctatataaa tatgatatta aacaatgggc agacaacgaa aatcttccaa tggaaagata
    14341 taaaaattat caaatgatag ttcctgcttt tataataaaa gatatattag atggaaaaat
    14401 tcctgagaca tttgaagatt caaagaatat aaaaatgatt gaagcgagtt ggggagaaga
    14461 atcgtataca aaagggcata tacctacaag tgttcatgta aataccgata ttatagaacc
    14521 tcctccaaca tggatgcttg ataatgatga taacttaatt aagtttgctt tagactatgg
    14581 attaacaaag gatgatactg taatagtttc aagtagtaca cctatggcat cttatagact
    14641 agctgttata ttgagatata taggagtaaa agatgtaaga gttttgaatg gtggaacaaa
    14701 ctcatggtta tcagcaggat atgaattaga atttactagt aatccaaaac attcatgtac
    14761 taattttgga gcagatatac cagttaactc acaattagtt gttactacct ctgaattaag
    14821 acaaaaacta aaagaaaaaa ataagtttat actagtagat aaccgtactt gggatgaaca
    14881 tataggaaaa gtatctggat atacatatta tgataaaaaa gggcgtatac caggagcttt
    14941 atatggacat tcaggtagtg atagtgtttc actagaagaa tatcgtaata ttgataatac
    15001 aatgagaaat aaatatgaga ttttagaaat gtgggacaag gaaaatatag atgtgaatag
    15061 acagcttata tttatgtgtg gaagtggatg gcgtgctgct gaagtattaa catacgcaaa
    15121 tgtcatagga gtggaaaata cctcccttta tagtgatgga tggatgggct ggagtcttga
    15181 taattcaaat ttaattgaag tgggagaaca caaataaagt ttttgaaagt acgatagagt
    15241 tgtttaaaac aaaggtgttt ttgatttatt gggaagagtg tgtaaattgt gttttgggaa
    15301 gtgtacttag gtatgttcat ttggagctat ggaaattgat aaaaaagtat tatctgttta
    15361 ttttgctggt aaaatgtgaa tagattatag attatagatt atagattata gatttaagta
    15421 tagactaagt aatttatatt acagaagaga atatataaga actagtgcaa gatgtatttg
    15481 atacatataa aaaattggca aatccagaat aaggatagag aaaattgaga ttgaagagtt
    15541 tgaaagagac ttattagatg aaaaatacaa agatgtataa aaatacatat tgagaaagta
    15601 tatctctctt aaatttgtta ataattctat tatacagaaa aacatattta agggggattt
    15661 tttactatga aaagagtaaa attagctata agctttttta tattaatgat tatagggatg
    15721 acaatatctt atgcagatac aagtataaat actaaataca atagattaag taaagctctt
    15781 gaaatttcag aagcaaattt taaattttat aatgttaaaa ttaattctgt tataaatcat
    15841 aatttatcaa ataaagaatt tcataatatt tttatagaaa taattaacaa tctaaagttg
    15901 gataaaaaag gaataaattg gaatcaaaaa gggtctggcg ataaaataca attatgtgct
    15961 actataaaag gtagcgataa agaaatttct ataaaaggaa taaataaaaa taacaaagaa
    16021 agttacatag tagttgacat attggataac aaagtatata aacataaaga ggttatttat
    16081 agtaacgtag aaaatgtgtt aaataaatat tcatctcaag tagatgtgtc tgcttgtatt
    16141 gttggagaat acacaaaaag gttacaaaac aacaaatatg atgatatttt acaaaaaata
    16201 ttatataata tgagtgctaa gcaaatagat gaagtgaaag acgtaaactt tttatctata
    16261 acagcatata gtaaagtatt aaatgattat gaattagaat atttagggga aaaaattaat
    16321 ttaaatatag gaattaaata taatgaggac gacggaagaa cactcatata tattgccaca
    16381 ccaattataa aattagatta ttaattatga ggagatcaaa aaatatggct aagataatag
    16441 taaaaaagag taatccactt aaaggtagtg ttaaaataga tggtgcaaaa aatgcagttt
    16501 taccaataat agcagcgact ttattagcaa acgggaaatc aacattaaat ggagtaccaa
    16561 atttaagaga tgttcatgtt atatcagatt tattaagaca tgtaggggca gaagttgaat
    16621 ataaagaaaa tacacttact gttgatgcaa gtaatataaa aacttgtgaa gctccatatg
    16681 agcttgtaag aaagatgaga gcttcttttt tggttatggg gccattgctt gctagattta
    16741 atagtacgaa aatttctatg ccaggaggat gcgctatagg aacaagacct atagatttac
    16801 atttaaaagg atttaaagcc ttaggtgcaa agatagaaat ggaccatggt tttgttgaag
    16861 cagcaacaga aaaattagta ggaaataaat tatatttaga tttcccatct gttggagcaa
    16921 ctgaaaacat aatgatggca gcttctttag cagaagggac tacaataata gagaatgcag
    16981 cagaagagcc agaaatagta gatttagcaa acttcttgaa cgaaatggga gcagatgtta
    17041 aaggtgcagg tactaatact ataaaaataa aaggtgttaa agagttaaaa ggagcagaac
    17101 ataatgtaat tccagacaga atagaagcag caacttatat ggttgcagca gctatgacaa
    17161 aaggtgatat aactgttgaa aatgttttaa tggagcattt aaaaccagtt gtagctaaat
    17221 taagagaagc tggttgtgaa ataacagaaa tggacaattc tgtaagagtt gtaggtccaa
    17281 aagtattaaa accaatagat ataaaaactt taccacatcc aggatttcct acagatgttc
    17341 aagcacaatt tatggctatg cttactgtag ctaatggaac tggtgtagtt atagaaactg
    17401 tttttgaaaa cagatttatg catgttgctg aatttaatag aatgggtgct aatataaaaa
    17461 tagatggaag aagtgcggtt gttaatggtg tagatgagct acatggtgca gcagtaaacg
    17521 ctactgattt aagagcaggg gcagccctta ttttatgcgg tcttatagca gaaggagaaa
    17581 ctcaaatagg agaaatatac catatccaaa gaggctatgt tgatatagat aagaagataa
    17641 cagcattagg tggtcaaatc gaaatagtag aagattaata attggtttat agaagaaagt
    17701 tataaattaa tagggttgtt catatactta aatacaaggt tttaaaagct aaggaggaga
    17761 gtatatgaag aacccattgg ttgttttgtt aggatttgtg acttgctctg tgttagttcc
    17821 atcacttata actttagttt cttataaaaa tgtagaatta actgaaaaag cagagagccc
    17881 agtatctata aacaaaacta ttaaaaaaag tgatattgaa gacaaaggta ataaagaaga
    17941 aaaaaataca gtgaactatg aaactgtaaa taaaaaagca ccaattatca atgtatataa
    18001 tcatataaca gggaaaaccg aaaagatgga catggagaac tatttatgtg gagtactagc
    18061 tggtgagatg tcttcagagt ttgatataga agccttaaaa gctcaatctg tagcagctag
    18121 aacatatgta gtatataaac aggaacatgg taaatctagt aaacataaaa atgcagtagt
    18181 atgtactgat tataaacatt gtcaagaata taagagttat gatactctta aaaaattaaa
    18241 tggtgaagaa tggattaaga ataaatattc caaaatccaa gaagcagtta gaggaactaa
    18301 aggtcaaata ataacttata acgataaggc gatacttcct ctttattttt ctacatcttc
    18361 aggaaaaaca gaaaatagtg aagaagtatt ttctgcaaaa tatccttatt taaagtctgt
    18421 ggaaagtcct tatgataagt attcccctaa atttgcgtca acacttaaaa taagcaacac
    18481 agattttgtt aaaagtctta gaagggcata tagcactatt gtgatagatg tgaataattt
    18541 aagtaaacaa gtttcaataa ctaaaagaag tgatgccgga actgtagaaa aaataaaatt
    18601 aggaaataaa gaattgactg gaaaagacat tagaactgtt tttaaattga actctgcaaa
    18661 ctttgacata aaatttggag aaggatacat agatttcgtg gtaaaaggat atggtcatgg
    18721 tgttggtatg agccagtggg gagcagaagg aatggcagaa gagggctata aatattatga
    18781 tatattatca cattactata cagatacaaa aataaaagat atatactaat aattgtataa
    18841 aatgccaaaa acacgtcaat aatccaaatg tagaaatata tttggaggtg caaaatgaag
    18901 aaaaagctgt tagaaaaaga tggtttttat ttatctttgt ttgtatgtgt ttgtttatta
    18961 gcagtaggtg gagtttggtt tacaaataat aatgtagata aattggcttc taataaagga
    19021 attatggaaa atgctaataa agatagtgaa gaggaaatac atttaattga aaaagacaag
    19081 aaagatgcta tccctactgc aacggattca aaacaaaact tagaaaaagc taagtcaaaa
    19141 gaggaaaata aatcgagtac aacaaaatta aattacattg gagataaagt aataagagga
    19201 tattcagaaa aagaacctag ttattctaaa acacttgatg tttgggaaac tcataaaggt
    19261 gtagatatta gttgtactaa aggcaaagaa gtaaaatctc tattaaacgg aatagtagta
    19321 gatgtattta atgatgaaga atatgggcaa tcagtcaaaa taaaaagtga caataacatt
    19381 gtagtagtat attcaaatct agataaaaat gttagtgtta agaaagaaca gaaggtaaca
    19441 gaaggacaat ctctaggtac tgtaggtagt acttctcaaa tagaaagtga agaaggtata
    19501 catgttcatt tagaggcgta tagtggagaa aaatctatag acccaatgag tctaattaag
    19561 taaaagtata acaagtttaa tcatatttgg ttaagcttgt tatatttttt ggtgttttta
    19621 catatatatc attaaagcta ttatgtgagg ggggtaatct ttgagatctc atatagagga
    19681 aagggccata gtggtagcaa aatatatact tgaaaaaaac actacagtaa gacaaaccgc
    19741 caagacattt ggagtaagta aaagtacaat tcataaggat gttacagaaa gattgaagga
    19801 aataaatcca tcccttgcca aagaagttaa aaatgtgctg gacaaaaata aatcagagag
    19861 acatattaga gggggattag caacgaaatt aaaatatgag aaggaacata aaaaaatgta
    19921 ggttaaaaac ctactttttt tatttaatta aattataaaa agaaatatat taagtaatta
    19981 taaatagcaa aataataata ctctcaaaag atttattttc tcttgattaa agcgagattt
    20041 ttgtatatgc tttattatag gagtattaaa ataggcttat aatataatct aatattataa
    20101 ataaaggagt tgaaaaaatg gctggagctg atataggcat agatttaggt actgcaaatg
    20161 tgcttgttta tgttagtggt aagggaatcg ttttagaaga accgtctgtt gtggcgatag
    20221 acaacagaac agattctgtt ttagctgtgg gagaagaagc taaaaagatg atagggagaa
    20281 caccaggtaa tatagtcgct ataagacctt taagagatgg tgtaatttca gattatgatg
    20341 taactgagaa gatgttaaaa tcttttatag ataagatagt tgataaaaag ggatttggaa
    20401 gattttttat gccaagaatt atggtatgcg taccaacagg tgtcacagaa gtggaaaaga
    20461 gagcagtaga agatgctacg agacaagctg gagctagaga agtatatata atagaagaac
    20521 caatagcagc agccatagga gctggtgtag acatatcaca gcctaatggt aatatgatta
    20581 tagatattgg tggaggtact gtagatatag ctgtaatatc tctaggagga gctgttgtaa
    20641 gtgaatctat aaaggttggt ggagatagat ttgatgatgc tatagttaaa tatatgaaaa
    20701 aacaacacaa tcttcttata ggagaaagaa cagcagagaa aatcaagttt gaaataggtt
    20761 ctgcatttaa gagagaagaa gaaaaatata tgaagataac aggaagaaat ttaattacag
    20821 gacttcctag ttctataact ataaattcaa cagaaatgtt ggaagcactt agagaatgtg
    20881 ttgagcaaat agtagttgct actcatgctg ttttggaaaa aacgcctcct gagttagcag
    20941 cagatatagg agatgttggt ataataatga ctggaggagg ttcattatta tatggattag
    21001 ataaaataat agaacagaga acaggaataa ctgttacaat agcagatgaa ccattatctt
    21061 gtgttgcaaa aggtacagga atagctttag gttcaataga cctacttgaa actggcgggt
    21121 ctttcaaaag aaaataatta ggagagttta gaatgttaaa tatagatgaa ataaaaaaat
    21181 taataccaca tagatatcct tttttattag tggacaagat tacagagcta gaagttggga
    21241 aaagagctgt tggtataaag aatgtaacag ttaatgaacc atttttccaa ggtcactttc
    21301 cagaatatcc tttaatgcca ggagtattaa tagtagaagc tttagcacaa gtgtgtggag
    21361 tggcaatgat gtctgtagag gaaaacaaag gaaaactagg tgtatttgca ggtatagata
    21421 aagttagaat caagagagaa gtaagaccag gagatacatt aactatggaa gttgaaatga
    21481 caactcttag aagaaatata gccaaggcag atgctaaggc atatgttggt gaagaacttg
    21541 tctgtaaagg ggaattaatg tttgccttag tagaaaaata aaaacttgaa atataaaagt
    21601 aaaaaggtag ataatttgga gtatttaatc agatactcta aaatatctac ctttttatat
    21661 aaagaatttt atcatttagt ttatatttag attaggtgga tactaataaa gccaaggcta
    21721 ttgtttcagc taagtctaat ataaagctaa gacgtatatt attaaatgaa agtttattat
    21781 tttcatcaat tttatctaca attcctacta tagaataatt tcctatttga ggtagttttt
    21841 tgccaacacc ttttccgggc aggataggtc cttctctaat ctgtatatta cctatgttac
    21901 tctgcgaccc caagcaagca tctatagcca agaagttacc ttgtatatgt gtatttttta
    21961 tagtatctaa agactcatat atatttaagg cgtgaatagg gttatctagt gtaccgtaaa
    22021 cggggtattt aaagttactg ttcttaagta ttgttccaac taaagggcct aaagtatcac
    22081 ctatagccct gtctgttcct atgcaaacaa caatcgtatt ttcattaatt atagttttta
    22141 gaactgaact tagcatctgt ataacatcat catcgctgta atttacttta gctaaataca
    22201 ataggaacac ccccttcaaa aaaatatatg aataaaaagc attaaaaaga cctaacttta
    22261 aatttcagat agatagaaaa ttttatttgt cttacaaaaa aaggaataga aaaaatatga
    22321 ctaaaaatat tgacaaaatt ttaaaaaaat agtaagctag taaagtaaaa caaaacttaa
    22381 atcttgatta ccttatcaag agaggctgag ggataggccc tgtgaagccc agcaaccacc
    22441 caaatgggaa aaggtgctaa ttcctacaag acaaaagtct tgaaagatga ggtcagttaa
    22501 ttagtatgta attacgatta ttaacctctt cttataaagg agaggttttt tattttgagt
    22561 aaatttctaa aattaaaaat aaggtaaaac ctataataaa aactaagaat aaataagggg
    22621 gaataaaaat ggcaagacat ttatttacgt cagaatcagt tacagaagga catcctgata
    22681 agatatgtga ccaaatatca gattcaatat tagatgcatt attagaaaaa gacccacaat
    22741 ctagagtagc ttgtgaaact acagttacta ctggtttagt tttagtagca ggagaaataa
    22801 gtacatctgc ttatgtagac attcctaaat tagtaagaga aacagtaaga gaaattggat
    22861 atacaagagc aaaatatgga tttgattgtg atacttgcgc agttataact tctattgatg
    22921 aacaatctgg agatatagct atgggagttg atgaaggcct agaaagtaag actggagaag
    22981 agatagaaga agaaatagaa aaagttggag ctggagacca aggtataatg tttggatttg
    23041 catgtaatga gactccagaa ttaatgcctc ttccaatatc tttggcacac aaattatcaa
    23101 gaagacttac agaggttaga aagactggtc tagttgatta tttaagacca gatggaaaaa
    23161 ctcaagttac tgttgaatat gaaggaagta aagctgtaag agtacataca gttcttatat
    23221 cagctcaaca ttgtgaaaca gtatcaaatg ataaaataag agaagattta attaatcatg
    23281 ttattaaaga agttatacca gcagaattac ttgatgaaga tactaaaatt tatataaatc
    23341 caacaggaag attcgttata ggaggaccac aaggagatac aggtcttaca ggaagaaaaa
    23401 taataataga tacttatggt ggatattcta gacatggtgg aggagctttc tcaggaaaag
    23461 accctacaaa ggttgataga tctgctgctt atgcagctag atatgttgca aaaaatattg
    23521 ttgcggctgg tcttgcagac aaatgtgaga tagaacttgc atatgctata ggtatagcta
    23581 gaccattatc tatatttata gatacttttg ggacaggcaa agtttcagaa gaaaaacttg
    23641 ttgaattagt aaataaacac tttgatttaa gaccaggagc cataataaga gacttagatt
    23701 taagaaagcc tctttataaa aaggttgctg cttatggtca ctttggtaga actgatatag
    23761 atttaccttg ggaaagaact gacaaggttg agcagttaag aaaagatgct ttaggtgaat
    23821 aatttttaac taaaaagctg ttagaatgta attttacgat tactttctaa cagctttttt
    23881 attatgagtt aaatattttt aataaatgtt aatttatcat catataatga ataaacagta
    23941 tttaatctgg tcaatacatt ttatataata ctatactaaa tttatgcata ttatgggata
    24001 tatgttaaag gatggttaag aatgtgttaa ctaatgttaa ataattaaaa atatattgaa
    24061 atatcataat aatgagtcta atataatctt aataaactta cactttaaaa ataattttaa
    24121 ttggggggag tagtgtatgt caggcaaagt aaatattaaa acggttataa gttttgctgg
    24181 agcttatgtt gctactgtaa taggttctgg atttgcaact ggtcaagaaa tactgcaatt
    24241 ttttacattt tatggatatg ctggtattat aggtggaata atctctatgg tactattttc
    24301 atggtttgga gcagaggtta tagataaggg aagagaattg aaacttaagg agccaataaa
    24361 aatttatcaa gtatattgtg gaaaatatct tggaacattt tttgaatggt ttggtccatt
    24421 gtttttattt ggaacattag taataatgat tgcaggagca ggagctacat tatcagagta
    24481 ctatggatta aatccatatg taggtaggat tggtatgaca atcgtttccc taataactgt
    24541 ttcattaggt ttgacaaggc tttctaaaat actaggtaat ataggaccta taataataat
    24601 atttacactt ttagtaggag caattagttt atttagcaat attgatgctt tatcaaatgc
    24661 tggaaatatg gttgatagtt taaatattaa aactgctaca tcaaatggat atttctcagg
    24721 agtactttat acttgttata atgtaataat agtaataaca ttcttgacag gaatgggagc
    24781 ttctgcagta aataagaaag atgctgtgtg gggtggtata gtaggtggag tagcacttat
    24841 ggcagcagct ataatgatga atttggcatt actttcagac ataggaaata tatacacaaa
    24901 agagatacca gcactttact tagctgataa gatatctcca ataataggta ttttattctc
    24961 agtagtactt ttactaggaa tatacactac agctgtacca ttgttatggt cagtaactaa
    25021 ccgatttgta gaagatgacc accctaaatt taaaattata acaatagtag taagtatatt
    25081 agcttgtata ggaggtctac tgccatttga taagttagta ggtacactat atccatatac
    25141 aggttacatg ggaattttaa ttttactatg tatgctttat agaagaatta ctaaaactga
    25201 ggggtacaag gagaataaat ctgaaataag ctagtactgt aatatactta tgaaatttaa
    25261 tttatactct aaatatggag taattaaaat aaaatagcta tttaaatatg gtacctgtat
    25321 ttcatggtac catattttat ggatgcagat tttttttgtt tctgataaaa tgtataatag
    25381 ttggaaatta cgttaagtat aaaaatataa aacttttcta cgattatcat agttttaata
    25441 tataaaatta tactaacatt taaatggagc gattattagt atttttagga gataaaaatg
    25501 gaaaaattag aaggaatgat aagtgaaata gtctttaaaa acgaagataa tggttataca
    25561 atagctcatc tggtaaatga aaatgatgaa attgtcgttg taggatgtat gcctactttg
    25621 gcaattggag aaagcataga agttgaagga aagtgggtaa accataagat ttatgggact
    25681 caatttgaag tgaatagttt tatgccagta acaccatcat ccttagaagg aatttatgtt
    25741 tacttatctt ctggaatgat acatggtatt ggtgaaaaaa tggctaaaag aataattgat
    25801 aagtttggtg tagatacgtt agaggtgata caaaattcac cagaaaaact acaagaagta
    25861 gaaggtatag ggagtaaaaa agtaaaacaa atagtaaaaa gttatgaaga agacagagaa
    25921 ttgagaaata taataataca gctatctcct tttggaataa caccaaatta ctgcctaaaa
    25981 atatataaaa aatataaaag cagtgcaata gaagttataa ataaaaaccc ataccaactt
    26041 gctgaagata taagaggaat tggatttaaa gtagctgata gtatagcaaa taaaattggt
    26101 atagataaaa attctaagga tagaatttgt cagggaatct tatacacttt aaataaatct
    26161 ttaagtaatg ggcatacata tttaccagag catgttctaa tacaagattc ggaaaaatta
    26221 ttggagctaa atggagaaat aataaaagag tgtgtaatga tgctagttta taatcaaaaa
    26281 atacatgtag agaaagtaaa caatgaaaat cttatatacc ttatgccata ctatttagct
    26341 gaaaatggag tatgtagtca aatagtaaaa ttatcacagt atgaatttga agacttaaaa
    26401 atagatatag ataacgagat taatgtttta gaagaagata agaaaattaa gttagcagaa
    26461 aaacaaattt tagcagtcaa agaatctgtt aatagtggcg tattaattat aaccggtggt
    26521 ccaggaactg gtaaaacaac taccattaat gctatcatag atatatttga aaataatggt
    26581 aaaagcgtga ctttggcagc tccaacggga agagcagcaa agagaatgag tgaaacttct
    26641 aataaagaag caaaaactat acataggttg ctagaaatgg gattttcaac agatgatgat
    26701 ttaacttttt ttaaggatga agaagaccca ataaattcag atgtaataat tgttgatgag
    26761 gtatcaatgg tagacattat cttaatgtac aatttactta gagctataaa gttaggaaca
    26821 agagtaattt tagttggaga tagtgaccaa ttaccttctg taggtgctgg aaatgtactt
    26881 aaggatatga ttaattctaa tattataaat gtagttaaat taaatgaaat atttagacaa
    26941 gcgcaagaaa gtatgataat tgtaaatgcc cataagataa acaatggaga accactttat
    27001 ctaaatacaa aaggtaaaga tttctttttt ataagaaaat caactaatga agaaatttta
    27061 aatgaaataa taggattggt taatgaaaga ttacctaaat tttataaagt tgataaatta
    27121 aaagatatac aagttttatc ctcaatgaga aaaggtgaat tgggagttac taatctcaat
    27181 atagagttac agaagtatct taacaaaaaa gaaaaattta aagttgaaga aagtttttct
    27241 aaaagattat ttagagttgg cgataaggta atgcaagtaa aaaataatta tacaaaaaaa
    27301 tgggaaacag aagaccaaaa agaaagtgga gaaggtatct ataatggaga tataggatat
    27361 gtttatcaca tagataaaga taaaaaaact atttatatat tatttgacca gactaaaatt
    27421 gtatcatacc tatatgatga attagatgaa attgaccata gtttttgtac aacaatacat
    27481 aaaagccaag gaagtgaatt tccagttgta gtattgccaa tagcttgggc tcctcctatg
    27541 ttacttagcc gtaatttatt atatacagca gtaacaagag caaaaaaatt agttgtatta
    27601 gttggagatg tgaaatatct tgaatatatg ataaaaaata atagagttaa tcaaaggtat
    27661 tcaaatttag gatataagct aaataaattt aagcaagaag ggctgttgat agaataaata
    27721 tgaaaatatg caatgataat tttattaaga attttaaaaa tatgattaat aaatgtttgg
    27781 attttatata tcctgagaat ataagttgca taatttgtga taaatccata aagaaaacaa
    27841 atacttattc aatatgtaaa agttgtttta aagaaatgaa ttttatacaa gatggttgca
    27901 tgaagtgtgg aaaaccaata atacgtcatt caattgaaaa agaatttatt gaagaatgta
    27961 gttattgttt taataaagac ttttattttg ataaatcaat atcttgtatt gaatacaatg
    28021 atgtgacaaa aaagatagtg ctagggctga agtataacca aaaaaccttt atggctaagt
    28081 atatagctca aataatgaaa gaaaaattat atttagaaaa cataaaattt gattatatat
    28141 tatttgttcc acttcataaa aaaaggctaa ataaaagagg ttttaatcag gcacaaaaaa
    28201 tagcatttaa tttaagtaaa atagtaaata ttccattatt ggattgtata agtagaaaaa
    28261 aatatacaag aatgctatat aaattgaata agaaagaacg aaaagaagaa ttaaaaaatg
    28321 tatttgttgt taaagacaat gttaaattaa taaataataa aaatatactt ttaatagatg
    28381 atatatttac tactggtcta acaacaaacg aaatttctaa acttttaaag ttatcgggtg
    28441 ctaataaggt ttttgtattg actctactta caaaagcaaa tgataattat attatggaat
    28501 aatcttatat attgacaatt ttaatttaaa atattaaaat gaataagtga tatttaggtc
    28561 tgtggttgaa agtccaagcc agtcgcaggc aaagggatcc acgtaagtta acactaaaaa
    28621 ttgggttaat gatcatggtg cggcttagaa gtaagaccta ccgataataa aatcgagaga
    28681 gagtagtagt gcggcgaacc caagcgaacc ctccagtagg cgagtgtggg gtcaaaaacc
    28741 aggtcagcta aatatcgtat taaaagggag ctagcatacc tttatgtata gctctttttt
    28801 atatataata aatttaggtt gagtcataaa gaggtgatat gtttgaataa aagacaggaa
    28861 aacttattaa caacgttgct attaaaagaa gagtttataa atgttaataa gattgccaat
    28921 gattttgagt gttcagaaag gacaataaga aatgattgca aatatattga tgaatggtta
    28981 tctacttttt gtaatgctta tatcaaaaga aaacctaata ttggaattaa atttagtgga
    29041 gatggaacag acaaaatact ggtaaatcag aagttgcgag gtgaagttaa aagagtaaat
    29101 agtgagcttc ataagcaagt tgagatatta aatttactac tttgtaataa taaaaaagtt
    29161 actttaaatg atttatctaa taagttgtac ataaataaaa atattgtcag agaagaaata
    29221 agtaaattat cagtggcatt aaataattat aatttaaatg tctcgactaa aaaaggcagt
    29281 ggtatatata ttggagggga tgagaaagac ataagaataa tgttagtatc attcttattt
    29341 aaatacatag ataaatacag attaaacata gatgaaatag agtacttcca tatagtagat
    29401 ataataattg taaaaaacat aatgtttact atagaggagg atatggatat aagacttaca
    29461 gatatatcat tcaagcaatt gatgctattt ttacttataa atataatacg aataaggcta
    29521 ggaaattctt taaaagtatc aaatactaag aaagagctta agctaaaaat gctgatatta
    29581 gatatagaag atgaattgaa atctaattta tccatagtat taaattatga agaaaaattg
    29641 tttatagagt ctttaatatt gggtatgaac aaacaaataa atagtagtaa tattaagaaa
    29701 tctttatatc taaatgaaga attagcaagt tatacaaaaa aaattataaa tttagtatca
    29761 gaagaatccg gaataaattt taataatgat atattacttt atgaacaatt aatatgtcat
    29821 ttgaatgtta caatgcacca aatgaaaagc aatgtttact tagaaaatcc acttttagat
    29881 aacataaaaa ctaaattttg ttttttattt acagtaatat caagttcaat agaaattgaa
    29941 ttaaaaggaa aacaaataac agaagatgaa attggttatc ttacacttca ctttcaaacc
    30001 tctctagaaa gaaaatacag caaaagggaa tcaaataaaa aggcaagtat tgtatgtcca
    30061 tttagttttg gagtaagcat gctattaaaa gtaaagatag aaaagagatt tgataacata
    30121 aaaattattg aaacattaag agaggaagat ttaaaaagag ataacttcaa taagactata
    30181 gattttataa ttgcatttca agagtatgaa aatatccaaa aaccaatttt tataacaaca
    30241 ccattattta ctgatgagga tgagaataaa cttaaagagt tcacaaacaa aataaaagaa
    30301 aaagacactt catataaact tatgaacaga ttaatgatgt ctgattattt aataagagag
    30361 ttagaacatg atgatattta tgaagctata acatatttaa ctaatttatt aattgaaaaa
    30421 cattatgcag aaaaagagta tttacaatct attataacaa gagaaaagtc atatccaacc
    30481 aatgttggaa aaggaatatt attccctcat ggagatatga aatatataaa acaatctgtt
    30541 ttatgttttg caaggttaaa aacaccaata agaattagaa atggaaagga tataaagatt
    30601 atacttttgt tagcatataa aaaagatgat gatagaaaag tatttagaga attatttaaa
    30661 gaaatatcta atttaacaga ggatgaaaat atgctagata ttctaagtaa atgtgacatt
    30721 gaaaaagtaa aagatattct tatataaaat tcatagtgaa ctatggataa gcacaaaatt
    30781 ttccatgtga tggaaaaagt tgtgcatgtt tttttgtttt tataagatat tatatgtttg
    30841 taaggtataa ataatctaaa agtagagaag caacttagga ggaaaaaatg gaagaaatag
    30901 ttttaaagat tataattcat gctggaaatg caaaatctat gctttatgaa gcacttgact
    30961 atgcaaagga aaacgatttt aaaaaagcag atgaattaat tgaaaatgca aatgaagaaa
    31021 ttttaaaagc acataaagta caaactgaac ttatacaaaa ggaagctggg ggagataaat
    31081 cagatatatc tattttattg attcactctc aagaccattt gatgacttgt atgagtgaga
    31141 gaaaccttat aaaagaaatg attatgttaa gaaaagaaat tcaaaaatta aaataaaaaa
    31201 attaaggggg atttaaaaaa tgaaaagaaa agtatattta ttttgtagct ttggtatgtc
    31261 aacaagttta cttgcagata aaatgcagaa ggtagcagat gaacacaatt taccaataga
    31321 agtagaggca tttccgatag cagaaattga caaaatagtt gaagaaaaaa atccagattg
    31381 tatattacta ggaccacaag ttaaatacat gctaaaggaa ttaaaaccaa agtttgaagc
    31441 tcaaggaaaa cttatagata taatcaatga agttgattat ggaactatga atggggaaaa
    31501 agtattaaaa ctcgctataa agctaataaa atccaaacaa gcgtagaatt atagtaaaaa
    31561 ataataggaa atgagggtag atagatgaat agattggaaa gaatacttat gccattggct
    31621 gaaaaaatag gtaaaaataa attattaatt gcaattagag atggtttttt aatatcatca
    31681 ccattattaa taataggttc attattttta ttagtagcaa attttcctat aaaaggatgg
    31741 aatgaatttg taggtcaaat ttttggttca gattgggctg taaaattgca acaaccaact
    31801 gtagcaacat ttgaagtaat gactctactt gcagttttag gaattggata ttcttatgca
    31861 aagcaaatag atgtagaccc aatagcatca gcagcagttg caatagttgc cttttttata
    31921 ataacaccat ttgtaatacc atatacacca gaaggaacag aatcagtcta catggtaaca
    31981 ggtataccac taggttggat gggctctaag ggaatgtttg taggtatgat aacagcaata
    32041 acctctgtaa aattatttgc tgcagttgta aaaaaaggat ggacattgaa aatgccagat
    32101 ggagttcctc caaccgtatc aaaatcattt gcagcattaa taccaagtgc aatagttatg
    32161 gtagttttct ttttagttaa gatagctttt gaagctacac catatggaag tatacatgaa
    32221 tttatattta agtttttaca aatgccatta ttaaaattag gaaatagttt aggtgctatg
    32281 gcaatcgctt atatattctt acatttattc tggttctttg gaataaatgg aagtagtgtt
    32341 gttggggctg tgtataatcc aatattaaaa attctatcag ctgaaaattt agctgctttt
    32401 caagctggtt caaaaatacc aaacataata acaggtcaat tccaagatat gtttgcgaca
    32461 tttggtggag caggtagtac actatcatta gtcatagcta tgttattaat atgtaaaagt
    32521 aaacgtataa agtcaatggg aaaactatca attttaccag gaatatttgg tataaatgag
    32581 ccattgatat ttggtctacc aataatgctt aacccaatac tattgatacc atttgcaata
    32641 gttccaacaa taaatataat aatagcttat ttctgtatgt cagcaggatt agtgcctctt
    32701 acaaatggag ttcagttacc gtggacaact ccaattatat tctctggttt tttaacaaca
    32761 ggatggcaag gtgcagtctt acaagtagtg cttcttatac taggaatatt tatgtatata
    32821 ccatttataa aaatgctaga taagcaatac ttaagagaag aaaatgcaac tgttgaagag
    32881 gaagatgatg atatatcttt tgatgattta gtattatagg aggaagatga tgaagaaaat
    32941 agttatgata ttggatcaaa tacaagctgg agctggagga aaagagaaaa gtaatatacc
    33001 tcctgctgga aaatcaagcc cattaggacc aggtataatg atggagcaat ttttaaatga
    33061 atctaaggta atagctacct tattttgtgg ggatgaattt tttgtaaata atgaagaaga
    33121 agtaactagt aagatgatag ctatggtaaa aaaattaaac cctgatgtag taatctgtgg
    33181 accaagcttt aattacgaaa acttttctaa gatgtcagca atactgtcta aaaatataaa
    33241 tgataaaaca gatataccag cttttgcagc tatgtcagaa gaaaatatag atgtgataaa
    33301 cgaatataaa aatgacatat gtatagttaa gactccaaaa aaaggtggaa ttggtcttaa
    33361 tgattcatta aataacatat gtaaattggc aaaagcaata gcaaataaag aagatataac
    33421 tttgatgaaa gaagaatttt gttattaata agagtattaa ttattagtaa gtttaaatat
    33481 tatactattt ataataagca tacattagta aaatctatat aatttaagga ggggaatttt
    33541 aacatgtggg gaatgatagc gacatggaga atggccctag aaggaataac tgaggcatca
    33601 gaacatttag aaaaaggcat ggaagcaggt gatgctgtag agcttgctgt aagaagagtt
    33661 gaagactatc cattctataa atcggttgga tatggtggac ttccaaatga aaattgtgag
    33721 gtagaacttg acgctgcatt tatggatggt aacacattag atattggagc agttgctggt
    33781 ataagagatt attctaatcc tgtcagcata gctaggaagt taagttatga gaaggtaaat
    33841 aattttttag ttggaatagg tgctgaagat tatgctcata aaaatggatt tgaaagaaaa
    33901 aatatgttga ctgatagagc gaagttacat tataaaaaga gaaaaaaaga gactttagac
    33961 aaaggattaa gtccatatgc tggtcatgat actgttggta tgatatcttt agacaaaact
    34021 ggaaaaatgt gtgcagctac atcaacaagt ggattgttta tgaagaaaag aggtagaata
    34081 ggagattctg cactatctgg ttcaggattc tatgttgata gtagtatagg aggagctaca
    34141 gctactggtt tgggagaaga tttaatgaag ggatgtatat cttatgagat agttagactt
    34201 atgaaagaag ggaagcatcc acaagaagca tgtgatattg cagtaaatga acttgataaa
    34261 cagcttatca aaagaagggg aaaagctggt gatttatcat tagttgccat gaataataaa
    34321 ggtgaatggg gagtagctac aaatatagct gattttactt tttcagtagt aactaaagaa
    34381 gaatctccta ctgtatatat ttcaaataga gtagatggaa agactataca taaggttata
    34441 tcaaatgagt ggatggaagc atatgaggct agaataaaag caccaataga agtaagtgag
    34501 taaagtagtg gttacataat taaaatagag ttttataata aggagatttt tatgaatatc
    34561 ataagtaata aaatagatga gttaaaagaa gatttattaa gtgatattat agatattgta
    34621 aaaataccta gtgtcaaagg ggagtcagaa aatggcttcc cttttggcga aaaagtaggg
    34681 gaggcactta ataaagcatt agaaatatca gaaaaattgg gatttaaggt tagaaacttg
    34741 gataattata ttggttatgc agagcatgga gatagtgatg attatgtttg tgtaattgga
    34801 catgtagatg ttgtacatga aggagatggt tggaagcatc agccttataa gggagaagaa
    34861 actaatggaa gaatttatgg acgaggagta ctagacaata aaggacctat aatgtctgcc
    34921 ttatatggat tatatgctat taaagaatta aatttaaaat tagataagag agttagaata
    34981 atatttggta caaatgaaga aagtggattt gaagatatac catattattt agaaaaagaa
    35041 aaagcaccta ttatgggatt tacaccagat tgtaaatatc ctgttgtata tggtgaaaaa
    35101 ggcatggcaa agattagaat aaaaagtaaa atcaattatg aagaagatgt atacttaggt
    35161 tttatagaaa atatgtctga aaatgtgctt gttacatata aagaactgaa tatagaaaat
    35221 agtgatacta tattagatat aaaggtaaaa tacgatttta gttataaatt gaaagatgta
    35281 ttggatgaaa taaaagctag ttttccaaat agtatcgata tagaggtaat ttctaatttt
    35341 aatcctgtat attttgataa agagagtaat cttgttaaaa agcttcagct agcttatgaa
    35401 agggttacat cattagatgg aactccagta acaactaatg gaggtacgta tgcaaaagta
    35461 atgccaaata tagttccgtt tggaccttca tttccaggac aaaaaggaat agctcataat
    35521 cctgatgaat acatggatat agaagatata atattaaatg caaagatatt tgcaaatgca
    35581 atatatgaat tagcaaagga gtagttatgt tagaaataat aggaatgtca gtagaagacg
    35641 ctaaaattat agaagattgt ggtgcagata gaatagagtt agttagtgct ttaactgaag
    35701 gtggattgac accaagtttt ggtcttatag aaagtgttgt taacagtgta aagatacctg
    35761 tgaacgtaat gataagacat catgcgaaaa gttttgtata cagtaaagaa gatataagta
    35821 taatgcaaaa agatataagt gtggttaaag agattggtgc taatggagtt gtatttggtg
    35881 tattggataa aaacaatagt atagatgaga aaaatctaaa tgttttattg aaatgttgtg
    35941 ataatcttga tgttacattt catagagcaa tagatgaatc aaatacaata gatagtgtta
    36001 aaattttgaa ggattatgat aaaattacga atatattaac atcaggaggg aaaggaagta
    36061 tagttgataa catacaaatg ataaagaata tgatgcttag ttctaatcac ataaaaattc
    36121 ttttaggtgg aggtcttaat tttaataata tagaaaagat aaaggaatta actaaggcaa
    36181 gtaattttca ttttggtaca gcgattagaa taaataacag tccttttgaa gatatagata
    36241 gacaaaagct taaacaactc gtaaatataa tttcaagata gtatatgaat ataaaatact
    36301 tagtttaaat aaagagagta agttaattat aatttgggta tatgaatgtt aataggtatt
    36361 aaatatgaaa ttattgttga gtatgcttta taacttttaa gtagttttaa atataaataa
    36421 tgaaagaaaa gatagattat ggaggtttta gagggtttat atttattaaa aaattataaa
    36481 ggtatattgg catataatac acataatttc ctaaaattac catcttatga catgaattca
    36541 catattaata aacaattaat ggagattttt gtggatagaa ataggagtaa aaatagcttt
    36601 aaataaacaa aaacaagagt tattaacagt tttatccaca ttatccacag gtgtttgtaa
    36661 taatattaat ccacataagt atcaatgtaa ctaaaaatga taaataattc tatcaattga
    36721 atagaaaaaa tcataaaagt aaataataaa aagaggatta tgagagttcg tgtagaatat
    36781 attattaaag tataaatttt cttatactgg cataattgaa gggagatgta tttatgaaca
    36841 taattatatc tggaaaacaa atcgaattaa ctgatgcaat aaaaaataaa atcgaaacta
    36901 aactaagtaa gttagataat tacattcatc cagacactga tgtaaaagtt acagtaagtg
    36961 cgagaaaagc aagacaaaaa atagaagtaa caatagctac tgtaaatggt cctataatta
    37021 gagcagaaga tatgcaggaa aatttataca cagctataga tgttgtatat gataaattaa
    37081 gtaaacaatt aaaaaaatat aaaaagagat tacaagataa acataaagac aacaagagta
    37141 taagattcca agatgcagag ataaatctag ctgatgaaca agaggattat ggggatgaaa
    37201 atcaagaatt agtaattcaa agacgtaaga aatttagtgt taagccaatg agtgaagaag
    37261 aagctgtatt acagatggaa ttaataggac atgattttta tatgttcaag aacatagatt
    37321 cagatgaaat agcagtagtt tataagcgta ataatggtgg atatggtata atagaacatg
    37381 aataaagcaa tttagattta atcatattag ctatcgataa ataaattatc gatagctttt
    37441 taaattaata ttaaaagtat agtttttatg gtaaaatgaa taatagtaca ataaagaacg
    37501 aggagagatg taaaatatga gctttatgga taatttattc aacatggcag ataaaaaaga
    37561 attaaaaaag ttcaataaaa ctgttgatat aatagattca ttagaaccta aatttgaatc
    37621 tatggcagac tcggaactta aaaatatgac aaatatattt aaagaaagat tagcaaatgg
    37681 tgaaagtata gatgatatac ttccagaagc ttttgcagta gttagagaag tttctaagag
    37741 agtgttagga cttagacact acagagttca aatgattgga ggtatagtac tacatcaagg
    37801 aagaatagca gaaatgaaaa caggtgaagg taaaactttg gttgcaactg ctcctgttta
    37861 tttgaatgcg cttactggaa aaggtgtaca tgttgtaaca gtaaatgatt accttgcaaa
    37921 acgtgataga gaccaaatgg caaaaatcta tgagttttta ggaatgagtg taggcgttat
    37981 tatacacggt caaaatccaa aagttagaaa agaacaatat gattgtgata taacttatgg
    38041 tacaaataat gaatatggat ttgactatct aaaggacaat atggtaatac acaaagagca
    38101 aagagttcaa agaggattaa attatgctat agtggatgag gttgactcta tactaataga
    38161 tgaggctaga acaccactta tcatatctgg acctggagac aaatcaactc acttatattc
    38221 agatgcaaac acatttgttt taaccttaaa accagatgat tatgaattag aagaaaaaga
    38281 taaggctgta tctctaacag catcgggtat acaaaaagca gaagtgtatt ttaatgttga
    38341 taatataaca gatatatctc atacagaatt ataccatcat ataaatcaag ctttaagagc
    38401 acatgtaata atgaagaaag atgttgatta tgttgctaaa gatggagaaa tagtaatagt
    38461 agatgagttc acaggtagac ttatgtttgg tagaagatat tcagaaggtt tacatcaagc
    38521 aatagaggct aaagaaggtc ttaaaataca aagagaatct aaaacactag ctactgtaac
    38581 attccaaaat tattttagaa tgtacaaaaa actttctggt atgactggta cagcaaaaac
    38641 agaagaagaa gagtttaaag caatctacaa aatggatgtt ttccaagtac ctactaataa
    38701 gcttatgata agggaagact taccagattg tgtatacaaa agtgaaatag gtaaatttaa
    38761 tgctgtagca caagaaataa ttgaaaggca caaagtcaat caaccaatac ttgtaggtac
    38821 agtatcaata gaaaaatcag aattattatc acaaatacta aagaaaaagg gcataaagca
    38881 cgaggtatta aatgctaagc atcatgataa agaggcagaa ataatagcac aagctggtag
    38941 acttggagct gttacaatag ctactaatat ggctggtcgt ggtacagata tagttctagg
    39001 tggaaatcca gactttttaa ctaagagaga aatgagaaga aatggattta aagaagaaat
    39061 agtaaataga gtagatactc caatagaagg tatacctgta aaaggtaatg aaattctatt
    39121 tgaagctaga gaagagtatg aaaaactatt tgaaaagttt aagcaacaaa cacaagaaga
    39181 acaaaaacaa gttgtagaag caggtggact tgctataata ggtacagaaa gacacgaatc
    39241 tcgaagaata gataaccagt tgagaggtcg tgctggacgt caaggtgacc cagggagttc
    39301 aagattctat ataggtcttg atgatgacct tatgagattg tttggtagtg atagaatatc
    39361 tggtattgta gataaaatag gtcttgaaga agatatgccg atagaacata gaatattatc
    39421 taagtctatt gaaggagcac aaaagaaagt cgaaggtaaa aactttggaa taagaaagca
    39481 tgtacttcaa tatgatgatg ttatgaataa acaaagagaa ataatatatg cagagagaaa
    39541 acgtgtatta gaaggtgaag atttacaaga acaaatacaa tcaatgactc attctataat
    39601 agaagaagca gttactcttt atacacaaga taaaggattt gatgaagaag gatttaaaga
    39661 acatatgtat aatctgttct tgccaaaagg tagcatagaa ataccagaaa tagaaaaact
    39721 taatccagtt gaaataacag aaaaagttta tgaaatagct atgaagatat atacttcaaa
    39781 ggaagaacaa gttggctatg agagaatgag agaagttgag agagtaatct tacttcaagc
    39841 tgttgataac cattggatag accatataga tgctatggac caattacgcc aaggtattgg
    39901 tcttcgtgct gtaggtcagc aagacccagt tattgcttat aaaatggaag gttttgatat
    39961 gtttgatgaa atgaataaac atatcaaaga agatactgta agatatttat ttaatataac
    40021 aatagaaact ccagtggaaa gaaaagcagt tgtagatgtt gaaaatctat cttctccatc
    40081 agatggaaca cttccaacaa gtaaaactgt taaaaaagat gaaaaggttg gaagaaatga
    40141 cttatgccct tgtggaagtg gtaaaaaata taaaaactgt tgtggaagat aatctcggag
    40201 gattaaatgt taaagttaca agagtataga catagtatgg agcagatggc ttcaaattta
    40261 gaagaattga gggtttctct ttgacatagc ttcattgatg aaaaatatac aaattaatga
    40321 agaaaagatg aataaacaag acttttggaa tgataatgaa gtcgctcaaa gagtacttca
    40381 agaaaataag tctttaaaag agacattaga agaatatgaa agcttaaaaa gcttactaga
    40441 agatatagaa gtattaattg aaataggttt agaagaagat gatgattctg tagaaagaga
    40501 tatagaaaag tccatagaat caatggaaga aaaattatct gaaatgaaaa taaagactct
    40561 tctaaatgga gagtatgata aaaataatgc tatattatct ataaatgcgg gtactggtgg
    40621 cttagatgct caagattggg ctcaaatgct tttgagaatg tatataagat ggtcagaaag
    40681 taagggttat aaagttaagc tcttagatat aatatcagac cctgaagcgg ggataaaaac
    40741 agctacaatt cttgtagaag gaacaaatgc ttatgggtat ttaaagagtg aaaaaggagt
    40801 tcatagatta gtgagaatat ctccatttga cccatcagga aaaagacata cttcatttgc
    40861 atctatagat gtaacaccag agttagatga aaatatagaa gtagaaatta atccatcaga
    40921 tttaaagata gacacatata gagcttctgg agctggaggg caacatgtaa atacaactga
    40981 ttcagcagtt agaataacac atatccctac tggtgtagta gttcagtgcc aaaatgaaag
    41041 gtctcagcat ctaaataagg acagagctat gagactttta atggcaaaat tgatagaatt
    41101 aaaagagtta gaacaaaagg aaaaaataga agatatacaa ggcaaatact cacaaataac
    41161 ttggggtagt caaattaggt catatgtttt tcaaccatat aaattggtta aagaccatag
    41221 aacaaatgct gagtttggaa atgtagatag tgtaatgaat gggaatatag acttgtttat
    41281 taatgaatac ttaaaaatga ataaaatagt ttaataaata tgcactgtaa ttaagatata
    41341 tgtataagaa ggttagtgct taatatgcac ttacagcact taggctgagg acgataaccc
    41401 tcagccttcg ttctgttttg ttatacttat gaaaaactaa cattaggagt gaataaattg
    41461 gatataaatc aaattttaaa aaaagagttt aatcttagag atgaacaaat aaataataca
    41521 ttaaaactta ttgatgaagg aaatacaata ccatttatag ctagatatag aaaagaaatg
    41581 actggcgaaa tgagtgatgt tacactaaga gaattttatg aaaagttaat gtatttgaga
    41641 aatcttcaaa gtagaaaaga tgatgtagtt agactaatag atgaacaagg taaattaaca
    41701 gatgaaataa cacaaaatat agaaaaagca aaaacactac aagaagtaga agatatatat
    41761 gctccatata aacaaaagaa aagaactaga gcaacaatag caaaagaaaa aggattagaa
    41821 aaattagcat tgtcaatact agagaataat ttagataata tagaaataga ggcaaagaat
    41881 tatcttgatg aagagaaaga agttctttct attgaagatg ctctaaaagg tgctagagat
    41941 ataattgcag aactagtctc agatgatgct aagattagaa agtatataag agaattagct
    42001 ttaagagaag gtatgatagt tagtaaaagt gccactgatg agaaatcggt ttatgacatg
    42061 tattatgatt atagtgaagc agtaaaaagt atgcctccac atagagtttt ggcaataaac
    42121 agaggagaaa aagaaaactt cctaaaggtt aagttagaaa taaataatga taaagtttta
    42181 aattacataa taaacgaata tgtaaatgat aaaaatttca aaaataaaga agaaatcgta
    42241 agttcaatag aagattctta taaaagatta attttcccat caatagaaag agaaataaga
    42301 aatcatttaa cagaaatagc tcaagaaaga gctataagtg tatttggaaa gaatgttaaa
    42361 agtttattgc ttcaaccacc agttaaagac aaagtagtta tgggatttga ccctgcattt
    42421 agaactggat gtaaaatagc tgtagtagat aaaaatggta aactactaga ttatacaaca
    42481 gtatatccaa cagaccctca aaatgatgta gagggagcaa agaaagtttt aaaaggattg
    42541 attgaaaaat acgatataga tataatttca attggaaatg gtactgcatc tagagaatca
    42601 gaaacttttg tatcagaaat gataaaagaa atagataacg aagtgcagta tgtgatagtt
    42661 agtgaagcag gagcatctgt ttattcagcc tctgaacttg caaatgagga acatccagat
    42721 ataaatgtat caataagagg agctatatct atagctagaa gattacaaga cccattagca
    42781 gagcttgtaa aaatagaccc taaaagtatt ggcgttggtc aatatcagca tgatttaaac
    42841 aaaaagagat tagaagaagt actagatgga gttgttgaag attctgtaaa tagtgttgga
    42901 gtagatttaa atactgcatc atactcccta ctagaacatg ttgcaggaat aagtaaaact
    42961 atagcaaaaa atataatagc atatagagaa gaaaatggag attttacgtc aagagcacaa
    43021 ttgaaaaaag taaagagact aggaccacaa gcatttactc aatgtgctgg atttatgaga
    43081 atcttagagg gtaaaaatcc actagataat acaggtgtgc atcctgaaag ttatgatata
    43141 tgtaaaaaaa tgattgagat aattggatat tctttagatg atgtaaagaa taagaatata
    43201 tgtgaaatag atgaaaaaat aaaagaaatt ggcttgagag aattaagtga aaaactggaa
    43261 gtagggcaag ttacgttaaa agatatcata actgaaatca aaaaaccagg aagagaccca
    43321 agagaagaag gtataaaacc tatacttaga actgatgtct taaaaattga agatatacaa
    43381 gagggtatga ctttaaaagg tacaataaga aatgttgtag attttggtgc atttgtagat
    43441 attggaataa aaaatgacgg attagttcat aaatcagaaa tgagtaatag ttttgtgaaa
    43501 gaccctatga gtattgttac agtaggtgat atagtagatg taaaagtcat tggaattgac
    43561 ttaaacaaaa aaagagttgc cttatcaatg aaaaaataga taagtttatg tatggttata
    43621 gtacttaaat tctatgtttg taaatgaggt tactcgagtg tataaatgtt aaaaatttgt
    43681 aaaagtatac agtaagaaaa tatattaaaa taagaacgat agtcttgaaa atcaggagga
    43741 aaatgaatga tttttataaa aaatggtaaa ataaatacca taacaaatgg gataattcat
    43801 ggagatatac ttatagatga aggaaaaata atagaaattg gagaagattt gatagctcct
    43861 ttagatgtag aggtaataga tgctagtaat aaacttgtgt ttcctggatt tatagatgct
    43921 catactcatt taggtttgtg ggaagatggt atagggtttg aaggagcaga tggaaatgaa
    43981 gagactgacc caataacacc acaattaaat cccatagatg gaattaatcc aatggataga
    44041 acttttaaag aagcttttga aggtggaata acttctgttt gtacaacccc tggaagtgca
    44101 aatgttatgg gtggtcaatg tatagctata aagacatgtg gaaaaagaat tgataaaatg
    44161 gtaataaaaa atccagttgc atctaaaatc gcttttggag aaaatccaaa gagctgttat
    44221 ggtcaggatg ataaatctcc tcaaactaga atggctatag ctgctttact tagagaaaac
    44281 ctaaaaaaag cagaagaata tttagaggat attgatatgt atgagagtca tgatgatgaa
    44341 gactgtgaaa aacctgaata tgatataaaa atggaaagtt taataccagt tttaagaaga
    44401 gaaattcctt ttaaggcaca tgctcataga gcagatgata tgtttacagc aataagaatt
    44461 gcaaaagaat ttaatttaaa gcttacatta gaccattgta cagaaggtca tcttattgta
    44521 gatgaattag tggaagaagg gtttcctgta atagtagggc catctttgtc agaaaggtct
    44581 aaatttgagc ttagaaattt gacatttaat acagcaggaa tactttcaaa tgcaggtctt
    44641 gatgtatgta taatgacaga ccatccagta ataccagttc aatatcttcc aatatgtgca
    44701 ggtattgcag tcaaacatgg tatgaaagaa gaaaaagcaa tagaatcaat tacgataaat
    44761 ccagctaaga ctttaggaat agaagataga gtaggctcta tagaagttgg taaagatgca
    44821 gacctagtaa tttgggataa ttctccacta gagattcaaa gtaatgtact ttacactata
    44881 ataaatggca aagtagtgta tgagaaaaaa taacttgaca attattaaaa aattaactaa
    44941 tataagagtg taaggaaaat taaaaaatca aagaagatct tcggggcagg gcgaaattcc
    45001 ctaccggcgg tatagcccgc gaactactta agtggttgat ttggtgagat tccaaagccg
    45061 acagtaaagt ctggatgaga gaagatattt atggaagata aataagttta ttttagtttg
    45121 tttaaactat atccataaaa actcgaagat agggtcttcg agttttttgt ttttcctaaa
    45181 aaatataaac atattgatta gggggaaata attatgcaaa acacagccaa aagaggcaat
    45241 atgatttcaa caagaacttt agtagtaatg tcaattttat ctgcaatatc ttatttatta
    45301 atgtttattc aagcaccaat accaggtata tttccagatt tcttaaaaat tgacttatca
    45361 gatattccag caatattcgg aggaatgtct ctaggaccat ttgttggatt tggaatagtt
    45421 gtagtaaaaa atgtgttaca agcaataaca gcaacaacaa ctggaggaat aggagaattt
    45481 gcaaatatat taattggtgg ttcttatgta atgatacttt gttattcata taagagaagt
    45541 ggagatttaa aaggtgtatt gattggtttt gtattaggaa caatatctat gactataatt
    45601 ggttcattag taaattattt tgtaatgttg ccactttatg gtcaacttat gggactagat
    45661 gctataatag gtttaggttc agctataaat cctcaagttc atgatttatt cacttttgta
    45721 atttggatga tagctccatt taatgtgctg aaggctgtta tactatcttt agtaacattg
    45781 ccactttata aaaaaatggg aaatgtaata aagaaataat tagacaagaa aatataaaat
    45841 taaatgtatt gaatagccat aatttattta taacttcagt cctatattgt gattgatata
    45901 ttaaatatat tatggcttta ttaatttatc aaaaaatgta tataaaataa gaaatattta
    45961 gacaaattta aattttaaaa taaaggaaga attaaaacga ttaactatta aaatatgaaa
    46021 aaggtatctg caatatacag agcgaattta taataaataa aaaataaagt tattattaaa
    46081 aaaatataaa gattagtaat caaattttta ataataacta atttaataat gatgggagtg
    46141 tatattcatg aacttatcta aaaagataaa aaaattattt ttagatgata aagggtatta
    46201 ttttaatagt aattggaaag atattcatat tggtatttat aatacaaaat taggagattc
    46261 tttaaagtat atcactaatg aatatattga aaatctggaa agggtaaaat atgactatcg
    46321 tgaattaaaa aatatagaaa atcaaactga tggtatgtgt attttagcaa taaggcagaa
    46381 ttacaaggca ttagagtatg taaaaaatca aaccgaagaa ttatgtttag aagcaataaa
    46441 gtataattat aaagcacttg aatatgtaaa agagcaaaca gaatatatat gtcttgaggc
    46501 tataaaaaag gattgtaatg cacttaagta tgtacgaaat aaaacagaaa ggcttattat
    46561 aaaagtaata cgtcattctt ccaatataga tgttgtaagt atactaaagg ctttagagac
    46621 tcaaacaaga agaatatgtt tagaagcaat taaaaaagat ggcaggtgtc ttgcatacgt
    46681 tagagaacag agtgaagaat tatgtataga ggccattaaa caaaattata aagcattaaa
    46741 atatgtagaa aatcaaacgg aaaaaatgtg tatagaatcc gtaagacaaa atggtatggc
    46801 attacaatat gtaaataaac aaactgataa aatatgtata gaagctgtca aacaagatgg
    46861 aagaagtcta caatttgtca ataataagac agaagaaata tgtataaatg ctataagata
    46921 tttaaataaa aaatataata ttaaagatgt actaagttac atagataagt atacagaaga
    46981 tatatgtata gagattgtaa gacaaaatgg aaaaatgctt atgtatataa aaaatcaaac
    47041 agaaaaaatg tgtattgaag cagtaaaaga gaactataag tctcttaagt atgtaaaaga
    47101 gcaatctgag agaatatgta aagaagcatt gaagcaaaac cataaagcaa aagagtatgt
    47161 aaaaattgct attgatgatt gtatataagg tgtagataca gccatcaata gtgaaagata
    47221 aaaaaagata aagacaagta ctttataata atctaggctt atactataat taattaagca
    47281 gtgatataaa ttcacatgac gattatatca aaatggagat gaattgatgg caaaaatata
    47341 tttagaaaat gaaaataaaa ctagagaaat aggatataaa ttaggtaaac ttttaaaaga
    47401 aggcagtgtt atctgtttag taggagattt aggagctggt aaaactacaa tgacacaaag
    47461 cttggcagat tcattaggga tagaagatta tataacaagt cctactttta ctataataaa
    47521 tgaatatgaa ggaaaaatac ctttgtatca ttttgatgtt tatagaatag gtagtagtga
    47581 cgaaatgtat gatattggtt atgatgaata tgtaaattca aatggaatct gtataataga
    47641 atgggctaat ctaatagaag atatacttcc aaaagaatat ttgaatatag aattgagata
    47701 taaagatgac ggaagagaaa tgatattaac tccaaaagga gagttttata aagagatagt
    47761 tgaggagttg attaaataat gaaaatacta ggtatggata cgtcttctat ggcagcaagt
    47821 gtagcagttg ttgaagatga taacttaata tgtgagttta cagtaaacaa caaaaaaact
    47881 cattcacaaa agcttatgcc aatgatagaa aatatgttga gtatgagtga tttaagtata
    47941 aaagatatgg atttattagc tgtatgtata ggtccaggct cgtttactgg acttagaata
    48001 ggtatggcca cagtaaaagc tatggctcat gtaaataata taccaataat agcagtaaat
    48061 tctttggaat ctctagcaaa taacataaat ttctgtgata gaaagatatg ttgtatatta
    48121 gatgcgcaga aaaatcaagt ttattcagct aaatacaagt ttgaaaatgg agagatgata
    48181 gaattagatg gtgtagatgt tgtagagttt gatgctttag tagatgaaat tgtatcaact
    48241 aatgaagaat ttataatagt tggagaagct gtatacaaat ataaagataa gttagagaat
    48301 ataaaaaata taaaaatacc ttcaccagct aataatgtat ctaacgcagg aagcttatgc
    48361 tctttagcat taaataaata taataaaaat atagatgtac atacttgtta tacgataaat
    48421 cctatgtata tcagaaaatc tcaagctgaa gtacaatatg atgaaaaaat gaagaggcta
    48481 aataatggaa gataatacaa aactaatagt agatgatata aaaatagaag aaatgactac
    48541 agaagatata gatgaagttt ttgaagtaga aaaaaattgt tttgaagatt attggtcaaa
    48601 agagtctttt agaaaagagt tgtctaatga agtagcaagg tatatagtag ctaagttaaa
    48661 tggaaaaatt gtcggatatg tgggaatttg gcttatactt gatgaagggc atataaataa
    48721 tgtggctgtt catagtgact atagagggaa aaaaataggg gacaagttga taaaaggcat
    48781 agttgattta tgtaaagata ataatattgc atctatgaca ttagaagtta gagcatcaaa
    48841 taaaatagca caaaatttat atagaaaata tggatttaaa atgggtggaa taagaaaaga
    48901 atactacagt gataataaag aagatgcaat tataatgtgg aaccaattaa aagaggtgta
    48961 gagtttatga gtgatataat aacattagct atagagagta gttgtgacga aacagctgca
    49021 tcagttttaa aaaatggtag agaagtactt tcaaacataa tctctactca aatagagaca
    49081 cataaaaaat ttggtggtgt agttccagaa gtggcttcaa gaaaacatgt ggaaaatatt
    49141 gatatagttg ttcaagaagc tttagacaaa gcaaatatag gttttaatga tatagaccat
    49201 atcgcagtta catatggacc tggactagta ggtgctttat tggttggtct atcttatgct
    49261 aaagcacttg catatacttt gaatattcca cttgtaggag taaatcatat agaagggcat
    49321 ctaagtgcaa actatataga gcataaggat ttgaaacctc cttttataac attaatagta
    49381 tctggaggtc atactcactt ggttgaagta aaagattatg gcaagtatga aattttagga
    49441 aaaacaagag atgatgcatc tggagaagca ttcgataaaa tttcaagagc aatgaattta
    49501 ggttatccag gaggacctat tattgataat ctagccaaga agggaaataa acatgctata
    49561 gagtttccaa gagcctattt agaagaagat agttatgatt ttagttttag tggattaaaa
    49621 tcttctgtat tgaattatct aaatgggaaa agaatgaaga atgaagaaat agtagttgaa
    49681 gatgtagcag catcttttca agaggctgtg gtagaggttt tatctactaa ggcactaaaa
    49741 gcagtaaaag aaaaaggata caatatcata actctttctg gtggagttgc ttctaattcg
    49801 gggcttagag ctaaaataac agagcttgct aaagacagtg gtattactgt taagtatcct
    49861 cctctaattt tatgcacaga taatgcagct atgatagggt gtgcaggata ttataatttt
    49921 ataaacggga aaacgcatga tatgagttta aatgcagttc caaatttaaa gataaatcag
    49981 tagagttaga taaatttgta aatataaaag tattgtttca aaaaagtaag cagaagtgaa
    50041 attaaatata ataaaaatca atatagtata tattaaaact tgaagtaaaa ctatttataa
    50101 tctttatatt ccaatgaatt gcaacacatt ttaggatata caggttgtct ttggcttaga
    50161 tttgcttcaa gtttaatttt ttctatagaa agattatttt aaaaagtggg taatataccc
    50221 ttttttttca ttcttaaaaa gttttatact aattatagaa agattaagga ggggaagaaa
    50281 tgagtcaaag taaagaagac aaaataagaa gtattttgga agcaaaaaat atcaaatcaa
    50341 attttcaaaa taaagaaaat ttaagtgaat ttaatgaaaa aaaagcaagt aaaagagcag
    50401 aagacttatt agatgtatat tataatacat tatctactgc agatatggaa tttccatatt
    50461 ggtataatag agaatataga aaatcagatg gtgatatacc agtagttaga agagctaaag
    50521 ctcttaaatc tgctttttca catatgacac caaatataat acctggtgag aaaatagtaa
    50581 tgcaaaagac acgccattat agaggttctt ttccaatgcc atgggtaagt gaaagtttct
    50641 ttgttgctca aggagaacaa atgagagaag aggcaaagaa attagccagt aacacagctg
    50701 atgaacttac aaaatttggg tctggcggag gaaatgttac tgaaagtttt ggaaatgtcg
    50761 tttcaatagc tggtaaattc ggaatgagaa aagaagaagt acctgtactt gtaaaaatgg
    50821 caaaagaatg ggttggaaag tctgttgaag atttaggttt tcactatgaa aaaatgatgc
    50881 cagattatga tttaaaagaa aacctaatga gtacacttat atgtatgttt gattcaggat
    50941 atacacttcc tcaaggtaga gaagttataa attattttta tcctttaaat tatggattag
    51001 atggaattat agaaatggca aaagaatgta aaaaggcagt tgcaggaaat gcatctggag
    51061 atgggcttat tggtatggac cgtctatatt tttatgaggc tgttattcaa gttattgaag
    51121 gattacaaac ttggatttta aattatgcaa aacatgcaaa atatttagaa agtatagaga
    51181 cagatttaga agctaaaaaa gaatactcag atttagtgga gatattagaa catattgcac
    51241 ataaacaacc tcgtacattt agggaagctc ttcagttgac ttatacaatt catatagcat
    51301 cagtaaatga ggatgctata tctggtatgt ctataggccg ttttggtcaa attttatatc
    51361 cttggtatga acaagatata gaaaaaggat taattacaaa agaagaggtt atcgaattat
    51421 tagagcttta tagaataaaa atcacttgta ttgactgttt tgcttcagcg ggcgtaaatg
    51481 gaggagtact atcaggaaat acatttaata ctttatctat aggtggatta aaagaagatg
    51541 gttctacagg agctaatgaa ctagaggagt tattattaga agcaagtatg agatgtagaa
    51601 ctcctcaacc tagtttaact atgttatatg atgaaaaact tccagaagat ttcttaatga
    51661 aagctgctga atgtacaaag cttggttctg gttatccagc ctgggtaaac aactcaaatg
    51721 gaacaacatt tatgatgaaa caatttgctg atgaaggtat gactgttgaa gaagcgagag
    51781 catttgcatt aggaggatgt ttggaaacgt ctccagggtg ctggaaacag ttaactttaa
    51841 acgggaagac atactctatt gcaggtggag caggtcaatc tgctggctct ggagttcact
    51901 ttatagctaa tcccaaaatt ttagaacttg ttcttatgaa tggtaaagac catcgtatga
    51961 atatacaagt atttgagcct cataataagc cattagatac ttatgaagaa gtcatagaag
    52021 tatttaaaga ttattataaa caagcaataa atgttttaga aagagcaaat aacattgagt
    52081 tagatatatg gagaaaattt gatacttcta taataaattc actattaaag ccagactgtt
    52141 tagacaaagg tcaacatata ggaaatatgg gatatcgtta taatgcaaca ttaaacgtag
    52201 aaacttgtgg tactgttact atggtcaatt catttgcagc acttaagaaa cttgtatatg
    52261 atgacaaagc atttacaata gaggaaatga aggatgccat attaaataat tttggattta
    52321 aagatgcatt agaagtagga aattattcta tggcagacca agtaaaagta gataagactg
    52381 gtaaatatga tgctatttat aaggcctgtc ttgatgcacc taaatatgga aataatgatt
    52441 tgtatgcaga taatattctt aaaaactatg aggtatggtt atcaaaagta tgtgaagaag
    52501 cacaatcttt atatgctaag aagatgtacc cttgccaaat atctgtttct acacatggac
    52561 cacagggagc tgctaccttg gctactgcag atggtagact aagtggaaca acatattcag
    52621 atggttcagt ctctgcatat gcaggaacag ataaaaatgg ggtatatgca ttatttgaat
    52681 ctgcaactat ttgggaccaa gcagtagttc aaaattctca gatgaattta aaacttcatc
    52741 caacaactat aaaagggcaa caaggaacta aaaaattatt agatttaaca agaagttatt
    52801 taagaaaagg tgggttccat attcaatata atgttgtaga ttcagaaaca ttaaaagatg
    52861 cacaaaaaaa tcctgacaat tatcgtcaat taatggtacg tgttgctggg tttactcaat
    52921 attggtgtga attaggtaaa ccaatacaag atgaagtaat tgctagaaca gagtatgaag
    52981 gggtgtaaaa tatgagaaag catagtgatt gtatgaattt ttgtgcagta gatgcaacca
    53041 aaggaatttg tagattatca aaacaaatga ttaatttaga tgatgcagca tgtccagaga
    53101 taaaagtaat gccaaaatgt aaaaattgta aaaattttgt tgaagctaat gatgaaggta
    53161 taggcaaatg tgttggccta gagaaagaag actgggtata ttcaacattg aatgcaatta
    53221 cttgtgaagg acatgtgttt aatgagtagt caaaagcaat tagaaggcat gattttcgat
    53281 gtacaaagct tttcagttca tgatggtcca ggttgtagaa caactgtatt tttaaatgga
    53341 tgtccactga gttgtaaatg gtgtgcaaat ccagaaagtt ggactgtaag accacatatg
    53401 atgtttagtg aattatcttg tcaatatgaa aatggatgta ctgtatgtca tggtaagtgt
    53461 aaaaatggtg cattgagctt taatcttgat aataagccag ttatagattg gaacatctgt
    53521 aaggactgtg aaagttttga atgcgtcaac tcatgttatt ataatgcgtt taaattatgt
    53581 gcaaaacctt acactgtaga tgaacttgtg caggtaatta aacgtgattc taacaattgg
    53641 agaagtaatg gaggagtaac atttagtgga ggtgagccac tacttcagca tgaattttta
    53701 catgaagtat tattaaagtg tcatgaggta aatatacaca ctgccattga aacgagtgca
    53761 tgtgtatcaa atgaagtttt taataaaata tttaacgata ttgattttgc atttattgat
    53821 ataaagcata tggatagaga aaaacacaaa gaacaaacag gtgtgtataa tgatttaata
    53881 ttagaaaata tatcaaacct tgcaaattct gattggaatg gaagattagt acttagagta
    53941 ccagttatct ctgggtttaa tgatagtgat gaaaatataa gtgatattat atcttttatg
    54001 cacaaaaata atttggtaga aattaatcta ttaccatttc acagattagg agaatcaaaa
    54061 tggattcaat taggcaagga atatgagtat tctgataagg gtgatgttga tgaaggtcat
    54121 cttggagaat tacaagatat atttttggat aatggtatag cttgttatgt tggtcatgag
    54181 acagctttct aaaattaaat acaagtttta attaaaaaag gatgttacac taaaagctag
    54241 agttacatcc ttttttatgt ggtttttatg tagaaataat taagtaaagt tttatatatt
    54301 ttatatattg tttatattgc tttatgtcca taaatattgc tttaaatata ctttatatat
    54361 tttttatagt ttaaattaca gttagagaat atattacgtt tactttattg aagcagaagt
    54421 tttgtggtaa aatactacta tgaaaaaatt taatagaata tattttagaa acaactaaaa
    54481 ataatagtta ggaggaagac atctatatga acaaatcatg gagcaacttg attattaaga
    54541 ataaagacaa aaaagtacgt tcagggtgga ttattatagc tgttatggct accttttaca
    54601 tatttcagta ttatttaagc atgatacttg tggaaataat aagaaaaatc ctggttgcta
    54661 caggggacat taatttaaaa acaaactatt tttcagaata tgctaattgg ttaaatgatg
    54721 ttgggttaca gattgtattt caaattctaa cagattcact tatgattatt attcctataa
    54781 ttgtatggag atttattatg aaaaatccta ttagtgaaat gggattacat tcttatagaa
    54841 ttaagaaaaa agaaggatat gtaggaatgt tattaggaat atttaattgt acagtaatct
    54901 ttattataac aataacaatt ggtggtggtc atgttacttc atgggtacca aaaatttcac
    54961 ctttaacagt ttcttgaata cttgtcttta ttttagttgc ttttgcagaa gaaattttaa
    55021 atcgtggttt ttttatggca gttttacgaa gatgtaaaaa tatttatttt ataatgattg
    55081 ttccatctgt tatttttggg ttgattcata tatggaatcc tgatgttact tttttgtctg
    55141 taattaatat tataattatt gggattcttt tttcatatat gtttataaag tcgtcgaata
    55201 tatggatgtg tataggttat cattttacat ggaatgtatt tcaggggatt atatatggta
    55261 tgcctgtaag tggacttcag gttccaggga ttataactac acaaatcact catggtaatc
    55321 tattaaatgg tggtatgttt ggtattgaag gtggaatttt gactactttt gttaatctac
    55381 ttagttttat atttgtatgg tattactata gagattctaa gtatgatttt gttagtgata
    55441 aagtaaatgt gaccaatatc aagaattagt tgattataat aaattttaat aatatcttct
    55501 aagcttaaag tattctgtag gattgttgga aatttacaaa aatataaaaa taacttggat
    55561 attctattta ctccaagtta tttttatatt cttttgtaaa tagaaatgga acataaaaaa
    55621 ttaaaacaac catagatact attagtatat catggtaagt gatagttgat tctttagaat
    55681 ttgtgataag aaaagcacta ttatttaaaa tatgaattat agcacaaaac cagatgttct
    55741 tagtttttat ataaagatat cctaaaaata ttgaaattcc aactatatgt atcgaacgta
    55801 atattaatcc taatattggt gtttcaggtg tatataatgt aaatattagt ggtaagtgcc
    55861 acaaactcca acaaatacct aatattataa ctcctagttt tttaccaaat ttattaaaaa
    55921 aaatattctg taaaaaacct ctccaggcat attcttcact aaaaaaaata taaatagtag
    55981 ttaatgatgt tattggagta aatataaatt ttatgatacc actataatta ggttgagacc
    56041 cttgtagtat taatataaat gatgttggaa caattacaga aatcataaag taaataatta
    56101 acaaagaacc tagtttaata ttttttatag gatataaatt ttttttattt gatgaatata
    56161 ttataattga taaactactt ataaaagtta atattaggtt tataattata aattcattat
//
LOCUS       DAEPIN010000019        53527 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.019, whole genome shotgun sequence.
ACCESSION   DAEPIN010000019 DAEPIN010000000
VERSION     DAEPIN010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 53527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53527
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.019"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            complement(<1..312)
                     /locus_tag="KR250_001356"
     CDS             complement(<1..312)
                     /locus_tag="KR250_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0067303.1"
                     /translation="MIYIIEILKISVINIFCFIGIFIVFGLIFSITENLNNKFIFSSF
                     GKTGIIVTGAIGTFVHELSHLIMCLIFMHKINSVKFFRPIESKNDGILGYVSHSYKKD
                     NL"
     gene            complement(342..713)
                     /locus_tag="KR250_001357"
     CDS             complement(342..713)
                     /locus_tag="KR250_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HBH0067304.1"
                     /translation="MKLKLLVEEYAVCRLNNDSKIPTWIDTEKFYSITRTDDELSVVC
                     LNNNIPSDVKSEKEWRILKILGPLDFSLVGILSKLSSLLADNKISIFAISTYDTDYIL
                     VKEKDIENACKILSCNGYEVE"
     gene            complement(866..1126)
                     /locus_tag="KR250_001358"
     CDS             complement(866..1126)
                     /locus_tag="KR250_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein S"
                     /protein_id="HBH0067305.1"
                     /translation="MEVLKVSSKSNPNSVAGALAGVLRERGVAEIQAIGAGALNQAIK
                     SIAIARGFVAPSGMDLVCIPAFTDIEIEGDKKTAIKLIIEPR"
     gene            complement(1315..2259)
                     /locus_tag="KR250_001359"
     CDS             complement(1315..2259)
                     /locus_tag="KR250_001359"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HBH0067306.1"
                     /translation="MIENNSEKIKVLENDIKQLITISKANNIDLSDKINSLNEKLEKL
                     KEDAFSHLSAYEKVTLSRDIKRPTTLEYIEHICSNFLELHGDRLFKDDPSMVGGIGQI
                     GKFNVTIVGHQKGRDTKENIKRNFGMPHPEGYRKALRLMKQAEKFNRPIITFIDTSGA
                     FCGLEAEERGQGEAIARNLLEMSKLSVPVITFVIGEGGSGGALGIGVGNDVCMLEHSV
                     YSVISPEGLSSILFKDSSKAKEACDVMKLTSNDLYDLKIIDKIIKEPLGGAQKDVEAV
                     SKEIKAYILERLNHYKDMDKEEIIAQRYKKFRNIGKCL"
     gene            complement(2280..3134)
                     /locus_tag="KR250_001360"
     CDS             complement(2280..3134)
                     /locus_tag="KR250_001360"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HBH0067307.1"
                     /translation="MIKKFLSSKESKYVTISLDDNFKKNSVDDKFWTYCKGCDSHVFR
                     KDIEENSFVCPKCSRHYGLRARKRINLLIDKGTFMEFNSDVEFQNPLNFPKYKEKVDS
                     YKEKTKESEAVVTGYGRINGIKTVICVMNPDFMMGSMGSIVGEKITYSIEYAAENNLP
                     IIICSASGGARMQEGMVSLMQMAKTSQALSKLEEKLLPYISVLTDPTTGGVTASFAML
                     GDIIISEPNTLIGFAGPRVIEQTINQKLPEGFQTSEFLLEKGFIDMIVDRRKMKEVLY
                     QILAMHKK"
     gene            complement(3149..4510)
                     /gene="accC"
                     /locus_tag="KR250_001361"
     CDS             complement(3149..4510)
                     /gene="accC"
                     /locus_tag="KR250_001361"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HBH0067308.1"
                     /translation="MIKKILVANRGDIAVRIIRTCKELGIKTVAIYSEIDKDCFHRYI
                     ADESICIGPNNISKSYNNIENIIYLALKLKCDAIHPGFGFLSENPEFAKQCEDNNIIF
                     IGPTREQMILMGDKSRARETMMELNIPVVPGSESVLKTKEEALEVAREIGYPVMIKAS
                     SGGGGKGMRIVRKEEELFSNFDMASSEALAAFSNSDLYMEKFIENPRHIEVQVFGDKH
                     SNAIHLGDRDCSMQRRNQKVIEESLSPYLSDEERQKLHKIAVDIVKGVGYIGAGTIEF
                     IVDKDKNFYFIEMNTRIQVEHPVTEMVTNLDLIKLQISIANGDKIPFKQEDITFRGHA
                     IECRINAEDSSKNFAPSPGKIESLNLPGGFGVRFDTFVYAGYTIPPLYDSMIGKLICW
                     AETREECISRIYRALDEIIVEGINTNVEFQKALVTSEEFREDTHHTKFIEDVFMKKEF
                     ATL"
     gene            complement(4526..4978)
                     /gene="accB"
                     /locus_tag="KR250_001362"
     CDS             complement(4526..4978)
                     /gene="accB"
                     /locus_tag="KR250_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HBH0067309.1"
                     /translation="MNINEIKELLKAIDSTNLEYVKLESSDLRLEVSKKAQSTSSPVL
                     SVQQESVVDLSLEKPVVNDTPVTSNENLSVVVAPLMGTFYDSPSPDADSFVKVGDVVE
                     EGDTLCILEAMKLMNEITSEIKGEIIEVLVSNEELVEYNQPLFKIKPL"
     gene            complement(5248..5694)
                     /locus_tag="KR250_001363"
     CDS             complement(5248..5694)
                     /locus_tag="KR250_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0067310.1"
                     /translation="MESKFFNWYTQSLGGTFGIISCICAYLNGYMFVYTNSASKYFES
                     IGFLGIISSYTLLPLCMLTLIFGVIYSYTPETSDKSIFNIPFFTLNKRLILITVVFGF
                     IGARVYFIIPAILITFNLYSNVLIAKLKYILCKNKENDTNLKESEV"
     gene            5969..6247
                     /locus_tag="KR250_001364"
     CDS             5969..6247
                     /locus_tag="KR250_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0067311.1"
                     /translation="MVDTLEFGLKILFFILSIIWMGKIMILRTDKQIVINPLLIGISA
                     VLVMLHTSQSNIEFFGLDVQYIRIVLYIIYSLIILIGIWATNRRNGIF"
     gene            complement(6376..7122)
                     /locus_tag="KR250_001365"
     CDS             complement(6376..7122)
                     /locus_tag="KR250_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0067312.1"
                     /translation="MWEGTMNLANLIPFIISAAALSPINSNDKVLSENQLKDIFPDAN
                     LRAVVKRYINPDEMTISNIKALDGEFYATGESISNLKGISYLENVDNFIFWNNNIKEI
                     PKEALSLKDMDSINLANNYLIDDDVVNSLSHNGVDVNCDLNFIDTKDNQYKLDSKYHN
                     VDIAKGESIDLRKIITKKIDSYYKYWEVTDNLPKDLNFIVSVSDKSVLSCEDMIIKGN
                     KTGQAVVKVILSDKNDLNTSQEVLITVNVK"
     gene            complement(7251..7895)
                     /gene="trmB"
                     /locus_tag="KR250_001366"
     CDS             complement(7251..7895)
                     /gene="trmB"
                     /locus_tag="KR250_001366"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HBH0067313.1"
                     /translation="MRRRRKKGADEKLLSYTKYVLRDDIDKLKGKWNLKFRNDNPIHV
                     EFGTGKGKFITTLAKQNPDINYIAMELKEEVLLKAVEKADASNLNNILFLWGDVSNIL
                     DYFEAKELSRIYINFCDPWPKNRWSKRRLTHSGFLEMYNRVLEDDGEIHFKTDNEKLF
                     EFSLNEIAANNWLLKNISLDLGNSEYENNVTTEYEDKFMSQGMRIFRCEAKKRN"
     gene            complement(8033..8353)
                     /locus_tag="KR250_001367"
     CDS             complement(8033..8353)
                     /locus_tag="KR250_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein HesB"
                     /protein_id="HBH0067314.1"
                     /translation="MKITLPETAIDTLKSILKDNQDKPNNIRVYFAGVGCGGPSFGLA
                     LDEKKEDDLTYEVGELQFVMSSDEYSQYGDIIIEDTGFGFRVIPENMKDQGGGGCSGC
                     SGCH"
     gene            complement(8806..9054)
                     /locus_tag="KR250_001368"
     CDS             complement(8806..9054)
                     /locus_tag="KR250_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBH0067315.1"
                     /translation="MQKTKAWGIIEPEKPESQQQMAHMDFAVNDLEEAVQYAIHCGAT
                     IAEEQFTDDWRVMIDPAGHPFCLCQMKSIMESSHFSLL"
     gene            complement(9085..9546)
                     /locus_tag="KR250_001369"
     CDS             complement(9085..9546)
                     /locus_tag="KR250_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/dioxygenase
                     family protein"
                     /protein_id="HBH0067316.1"
                     /translation="MKFGGVLLIVKDINKSKKFYEDVMEQKITLDLGEHVSFENGFGL
                     QSNYKAIIGYDLPIYQQSNNFQLYFEVNDIKQWESKINRIGNIEFLHYIKEYPWGQRT
                     FRFYDFDKNIIEISESMESVIKRLLKQGLALEEISKRTMYPVEFITQFQQL"
     gene            complement(9584..10417)
                     /locus_tag="KR250_001370"
     CDS             complement(9584..10417)
                     /locus_tag="KR250_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0067317.1"
                     /translation="MLKKESIHFINKNRSLIVCEDNFVYLLPCIQLRNWISNFTISFP
                     NQTIISDNYTIIPHGSVTLVFFYDATGLHSLLFGPTTKPKTVGNIANRCDVILIIEFQ
                     PAGFFPLIGIQQSELIDKVVPFSIINTSLDLEIKKIFNESLSIDKLILKLEELLISNI
                     KIEYSYEFILAIQLIIQNSGNISSQEISKKVFYSSRHLNRLFNQYLGLSMKSFSRLVR
                     INKSIKLLNNNKTSLMSICNELGFYDIPHFIKDFKIVCGITPQKYRANMSDFYSEIAK
                     F"
     gene            complement(10691..11377)
                     /locus_tag="KR250_001371"
     CDS             complement(10691..11377)
                     /locus_tag="KR250_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0067318.1"
                     /translation="MKITHNSLAVEAKNIIKEYKIGNTTTRVLKEVSLQVMKGEFVSI
                     MGQSGSGKSTLLYILGGLDTPTSGKVYMNGADISHFNDEKMSIIRRRNIGFVFQFYNL
                     IPNLNVEENIMLPLLLDGKNLKDYKNQLDEILDIVGLTDRRKHTPRELSGGQQQRVAI
                     ARALIGKPEILFADEPTGNLDSKTGIEIIDLLNKINRDNGQTIIMVTHSPEAAKSSSR
                     TITVSDGLIV"
     gene            complement(11392..13869)
                     /locus_tag="KR250_001372"
     CDS             complement(11392..13869)
                     /locus_tag="KR250_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBH0067319.1"
                     /translation="MKIILKYIFTNIKERKIRTAVMLLSIVLSTVLLFVSFSIGLSYE
                     SAQRKMAKGMYGTATISVQSKNPDILTNLEDIPDLNAIKSKVGVLESSAIYNKGGYYE
                     EFSLISADLSQLNKINKPRLENGDSITDFSGDKIILPNRFTSKYKIKKGDSITLQIYG
                     KSYTFQVSDIASYDTVFLRNTRGVNALLPKETLSKIINKGSGYTRVLIESEEEITENL
                     VNKLSKELSTEKYMVSNTINETKIISDARQKTMPFFLISFFALTLSIFIIYSSYKVIT
                     LERLPFIGTFRSIGANEKTVTRILMLESILYGSIGGLIAIPIGVVVLNLMLHGLGNSL
                     EQGISIPVVISPIGVIISVIVAIIVSSFSAYIPVKKASHLPIKNIVLGTVEEKNVSNR
                     SILFIGSIMFILSILLPRISPENTLYLAGGFSLLGLIVATIVLIPLITDIMSIVFEFV
                     YKNILGNEGKLAARNMKNNKNIIQNITLLFISISAVIAISVVGNFVKTYITDVFRDAE
                     LQGFADGKMNEEFIEDVRHMDGIKKILPLYVMNNEISGNGVTLSRLEGTDNIKLYNSM
                     FGINYTNFEIKKQVIEAFNDKRSVILNEDTLKKVGLSIGDTITLSNDKYDFSYKIVGS
                     FKSRANDVEAVIPSHYAVNDFDKTNYGFLVYTAVNPDAVMIQIRYLFGDTYNWSRTVE
                     EFNNDSLNTISSFLSPMNKMTYFIFLLATVGIINNLLINYIQKRRSIAMYKSIGLSNK
                     QNIKVTLIEGFTSGLLGAVIGIVISILEIQTIFIVAGPKISMKPDLDFKTFIIVGLLG
                     IIVTLIGSIVPIIKGKKMKLIEEIKFE"
     gene            complement(14088..15491)
                     /locus_tag="KR250_001373"
     CDS             complement(14088..15491)
                     /locus_tag="KR250_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBH0067320.1"
                     /translation="MHKPSYKTVFHSYIIFFMIFICLILTVIGIFLSLITIQKPNGEF
                     IRSDWPKKFTENFNEQIVFIDNKPQVKQSGLEKLRENQLWLQILDSSGNRVYGFLEPK
                     EHKDYYSNSELLALANQNNSSNGTVYFNTIKNNKNDYIYIIYFPLNISKVNMFFDGGK
                     FIGGKSIVFSIIGIIFLIILVSGIAYGYWVTKCISNITSAVSDISKRSYLPVNIKGVF
                     ADIYNSLNILNEEIHRSDEIQNKTDIMREEWISNITHDLKTPLSPIKGYAELLTDSST
                     NYSPEKVYKYASIMLKNIEYTNTLINDLKLTYQLKNGIIPLNKNNYDLVRFLKELSID
                     ILNYPEYESNIVEFKSNIDNINFDFDCTLLKRAFSNLIVNAFTYGDKNTKVCVHITLN
                     ERIEIIISDNGKGMSREEASNLFNRYYRGSNTKQKIEGTGLGLAITKQIIEVHGGNIS
                     VESIPGLGSSFYITFPI"
     gene            15984..16844
                     /locus_tag="KR250_001374"
     CDS             15984..16844
                     /locus_tag="KR250_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBH0067321.1"
                     /translation="MKKQKSPILLEMLGLFFGCISMSIGINMFLKPHTIAPGGLSGLS
                     LVLNKVTGLPVSAIMLIIGVPLVILAFRIMGTKNSLKTLFGTVVFSAIVQLTDPLSKL
                     RFTNDLLLSSIAGGILVGIGLGIMFKSDASTGGTDLIALILSKKFPGIKATKFMSCLD
                     GMVVISSGIVNRSLETALYSGIALCVLIKIADMIMEGFDHSKAFFIISDEPESLRQAI
                     TEELDRGLTILDGKGGYTRENKEVLLVVVSKKQELYLKRLVKRTDPTAFVIVTDVHEV
                     LGEGFKNLEN"
     gene            complement(17083..17640)
                     /locus_tag="KR250_001375"
     CDS             complement(17083..17640)
                     /locus_tag="KR250_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBH0067322.1"
                     /translation="MSDSEYPQMKTLETRNCILRPASLDDVEDFFDCYKNKIVVKHLP
                     FSEHKSLEDTRKFIKSFFLNPYKKGKIGHFAIVYKRDNKVIGNMGFNNINPKALEAEI
                     GICINPSYWGHDFATEITKRVIQYGFRELNLNKIIAITYEENQNSTKSLDLLGFKCTG
                     KYIKKIHTGNTVKNVPCYQYELKKQ"
     gene            complement(17830..18717)
                     /locus_tag="KR250_001376"
     CDS             complement(17830..18717)
                     /locus_tag="KR250_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBH0067323.1"
                     /translation="MNIKLELYKVFNAVVNNKSFSLAAKELFMSQPAVSQSIKQLEEQ
                     LDTLLFYRNNKGVKLTPEGKILSEHVTTALKLISSGEDRINKFKKLEYGSLKIGVGDT
                     AARFFLLKYLEIFHKKYPHIHVSTINRTSRELISLLKDGNIDIAIINMPIEDDTLNIV
                     ECIEIHDIFVCANDYIEYKGRKISLEELNTLPLIMLENKANSRLYVNEYFLSKGIKLN
                     PDIELGSHELLLEFAYINLGVSCVIEEFSIDYLENEKLFKLDIKEPIPKRNIGYCHLK
                     DISLSLATKEFLSMISNNI"
     gene            18836..20035
                     /locus_tag="KR250_001377"
     CDS             18836..20035
                     /locus_tag="KR250_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003493023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HBH0067324.1"
                     /translation="MDRLVGTVSRGVRAPIIRQGDDLVKIVVDSVLNASKSENFEVRD
                     KDVIAVTEAVVARAQGNYAHVDNIAKDVKDKFGDDTVGVIFPILSRNRFAICLKGIAK
                     GCRKVVLMLSYPSDEVGNHLISIDELDDKGINPWSDVLTEEKYRELFGYNKHTFTGVD
                     YVDYYKNLIKDCGAEVEIVFANNPKTILNYTTSILTCDIHTRQRTKRILRQNGAKKVY
                     SLDDIMTASVDGSGYNDQYGLLGSNKATEETVKLFPINCDEVVNKIQGNIKEITGKDV
                     EVMVYGDGAFKDPVGKIWELADPVVSPAYTKGLEGTPNEVKLKYLADNDFADLSGDEL
                     KEAISKYIVEKDNKSDDLTGNMVSQGTTPRRLTDLIGSLADLTSGSGDKGTPIIYIQG
                     YFDNYTK"
     gene            complement(20229..22784)
                     /locus_tag="KR250_001378"
     CDS             complement(20229..22784)
                     /locus_tag="KR250_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBH0067325.1"
                     /translation="MMSLVKFSIQYIKHYKKQSISIVLSIVLSVALLTGIGSLVHSAD
                     KSRIEKIREDSGDYHYFYKVDKEQLNKIKENKKSKEYTVDRLGVTKPKNQIDEPFIIE
                     FLNADSSYLDMTGRKLLKGTLPSKDGEIVLDTYSLNNLNVNKEIGTEINLDGKIYKLC
                     GILSSLSNPETALEGFVNDYFQPKEKDSYIVYVKYDENKNIKKQNYKFMKKFNISKEK
                     TYINWSLSVEFGVKPPKNIEKYNIFEFLKNLELNTNAIILLVGVFGAAAVYSIFHVSI
                     LQRISQYGVLEVLGANNKQLLFLLFLELFLLFIVGFPIGCFLGIGVASTIYEQFPHIF
                     LSSDIVPGAFFISEKSIYLGFLFILLLLILIAVRVVHQLNKYSSIESMKNFKITNKKK
                     RILESVKHSNMTKVLSHKYMTQKKGMFIGILFSLSLGGIIFLCSSYSIQLTKNNNELT
                     MKADDGLNSDYQITMQTTDFDVGIPKDKISRLNNVEGISKVYPVRYLFGAMQIKEEQL
                     FWKNFFKPLEKDYRIKTFFDGICTKQNEGGYLLKTNIYGYNNGMLEGLNSYIIDGEIN
                     THDMVKNNKVIVRLPMDGTGMYDAVDINPGDTIKVKVPKTMKPTDEIVKFKEENKNDY
                     TTKEFVVAATVKRVMANNIYFIGDYGMDIVMTNGQMKNNFDIENYNSVSIKKTEDSNS
                     EELSENIKSVVSDVKRCIVTDYTIAIEKNNTYLNQKLLFIYGIVFVLLAISLFHIINT
                     VSYLIFSRRHEFGILRAMGITDNKFLLMMIREGFLYGFYASIIMVIGSVIGQFMIYFM
                     VKRVYLYINPILKINTPLYIGMIILNITISIVAVIIPVRQILKSDIISEINKN"
     gene            complement(22781..23467)
                     /locus_tag="KR250_001379"
     CDS             complement(22781..23467)
                     /locus_tag="KR250_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0067326.1"
                     /translation="MSILVTNNLVKHYGKGETKVKALNGVSIEIERDTFTAIVGTSGS
                     GKSTLLNIIGGLDNPTSGDVIIKGKNISKIGKKDLTVFRRRNIGFIFQNYSLMPVLNT
                     YDNIALPVTLDKGNHIDHEYIEMLMKTLGIWDKRLKFPSELSGGQQQRVAIARALANK
                     PALILADEPTGNLDSKTTMEVVCLLKESSAKFHQTILMVTHNENIAQVCDSIIHIEDG
                     VVVNTGGELL"
     gene            complement(23544..24452)
                     /locus_tag="KR250_001380"
     CDS             complement(23544..24452)
                     /locus_tag="KR250_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBH0067327.1"
                     /translation="MFILAFIICTVVIFIVSTKINRKRYDELNLMLNQILEGKEVTYP
                     DTKDTRASKISHQVKKIKDMIEIEVEQSKSEKEAIKGLISNMSHQLKTPLSNITIYCE
                     LLENINISTLQKKEFLQKMKNETFKIDWLLQSLFKMTKLEDGVIEFEVEELLIKDTLI
                     QSISTIFNKAEAKNIRINLEPFSDIKLVHNKKWTIEAIVNVLENAIKYSPSDSTITIS
                     VIKMELYTKITIKDEGIGIDSRELNDIFKRFYRSKNVENQNGTGIGLYLTRLILEKEN
                     GNIIVESKLGSGCCFSIFLQNCKSLN"
     gene            complement(24454..25125)
                     /locus_tag="KR250_001381"
     CDS             complement(24454..25125)
                     /locus_tag="KR250_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0067328.1"
                     /translation="MNLLIIEDDINLNEGLFYAFENDGFNVFKAYTKQEGLNIFNSKN
                     IDFIILDCNLPDGDGFDVCQIIREKSDIPIIMLTARDSEIDEVKGLEIGLDDYITKPF
                     SLSVLKARVKVAIRKKSNNKVIYSNGIKLDQKLLKVYKNKECLELSSVEYKLLSYLIE
                     NKGQILLKEQILHHIWDSEENYVDDNIVSVNIRRLRVKVEDDPSNPKYIKTAYGMGYL
                     WNEVE"
     gene            25387..26451
                     /locus_tag="KR250_001382"
     CDS             25387..26451
                     /locus_tag="KR250_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S ferredoxin"
                     /protein_id="HBH0067329.1"
                     /translation="MKRVDERNTMFARANYKKDSTAYNDYYKKNPDKKEIDDSIRNRP
                     NLCSEGTMTYNELNSPMASSAFDFLSDIKSLCEGKVSDTKVDVDAKSMTKKIKGLAKQ
                     YGASVVGITKLKDYHIYTNRGRHEENYGEEINLTHKYAIVFGCEMDKEMINRAPMICE
                     VIETSKCYVDASIVGMILSYYIRNLGYDARNHMDANYLVMPVFIARDAGLGDIGRNAI
                     LTNKDYGSRLRLGVVTTDIPLLEDEYVDFGLEDFCKVCKKCSFNCPSHSLSNDIKIGD
                     DGKYNWVIEHETCYIKWRYLGTDCGMCISACPFSQNLETIKNTTSFKGNNELIQKALD
                     EYTSKFGKRIFIPGNPSWLK"
     gene            26609..27295
                     /locus_tag="KR250_001383"
     CDS             26609..27295
                     /locus_tag="KR250_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0067330.1"
                     /translation="MEKILVVEDDSILNKTLSYNLIEDGYIITSKFTAKSALKSIFEC
                     EFDLIILDINLPDKSGFELCNEIKENYNIPIIFLTANDMECDMIKGYELGALDYITKP
                     FNINIFKQKIKAFLNHLTIKTKQDYYKDGYLEINFSELSANINGNQIIFTPLEYRTLK
                     LLTENPKSILTRKVLLEKLWDIDANFVDEHTLTSVISRIRSKIEKDNLKYIKTVYGMG
                     YMWLGERNEF"
     gene            27285..28316
                     /locus_tag="KR250_001384"
     CDS             27285..28316
                     /locus_tag="KR250_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBH0067331.1"
                     /translation="MNFKKFSFSSIYLIIAVGIIFTAILFSISIYLITGDLKLIALLL
                     IYTMVLCGFIALIIFLLHKKIVIFCSKICHTLDSMMNSEDNIEIELEEETILSRINHR
                     LIRMYEVIQENRNSIAIEKADLQELVSDISHQIKTPIANLKMINTTLLNQTFKTEIQK
                     DFLMDMENQLDKLDFLMQSMVKTSRLETGIITLSKKKNSIYETIATALSGILFDAEKK
                     NIEVTVDCDPNLYIIHDKKWTSEAIFNILDNAVKYTSSNGKIRVATECWVMYTKIDII
                     DNGKGISESRQAEIFKRFYREEDVHDIEGIGIGLYLSRKIISLQGGYIKVTSDIGKGS
                     TFSIFLPNK"
     gene            28398..29063
                     /locus_tag="KR250_001385"
     CDS             28398..29063
                     /locus_tag="KR250_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0067332.1"
                     /translation="MSILQVINLKKYYGQSSNITKALDGVTLSIEKGEFISIVGTSGS
                     GKSTLLNMMGGLDNPTSGKVIIKGKELSTMSDEQLTIFRRRNIGFIFQNYNLVPLLNV
                     YENIILPIGLDGETIDKDFINEIINMLSLEEKLYDIPSNLSGGQQQRVAIARALVTKP
                     AIILADEPTGNLDSRTSSDVLSLLKVTSKKFNQTIVMITHNNEIAQLTDRIIRIEDGK
                     IVN"
     gene            29076..31493
                     /locus_tag="KR250_001386"
     CDS             29076..31493
                     /locus_tag="KR250_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0067333.1"
                     /translation="MFRNNNRTFIRKIALNDLKINKLKTYLSGIIIMISTCLLLTVTL
                     VSYNASVDLVNASPYHAIYKSVDEKAKNILYKNKNFSGVGTYKLIGSNKKTDCIMSIV
                     YADDTAIKLMNFQPLKGKLPTNKNEIAISEKYLQEFGLDKDIGDSIKLNYYDDITNKE
                     VQCDFIIVGFLENYYQDNAKQYYTVVSNDYFKNIASTPLQNKSNTFDESRPDTVDVLV
                     KLSEENTEKDSASIKTQLNKIGLSLGIKKYNIYLNDNYIESNLIDGEQIITVLVVGIV
                     VLFSSVFVIYSIFYISVVNSVQMYAKLKSLGMTSFQLKKIISLQGNILSIIFIPLGVI
                     ASCIIAYIIQPLAWQMIADLFIILILSLVMFLTVRISLFKPARIISKISAIEAMQYTE
                     TKFRKKAKNFSYINIKNLALKNIESNRKKNLIALISLSISGVLFISIANLANSIDFKK
                     QLSQQFVYNEDYIISIKSDNLYEHITEIQQNNPLTDSLKNEIKSIHGVKKLIESKSIK
                     SNIIEPYIKDRDEKQFITLIKGITPELSKSLEEYIVSGKIDYTHLDSDSLIINKYRTK
                     YYGLNLKVGDTVTFNIFSGNDIIKKKMKIIGIIDNSNTGGMFFASDKTLESLTPYNTN
                     LDFSILIDKKHSKEVEEKLLSIIQKNSNLMLYSYEDDYRMITRAFQYIIIASYIFVGL
                     ISCFGILNMTNTLINSVLIRKKEFALLQAVGMTRKQLRNMLYREGLNISIKAICTSST
                     LGYFGSNLLCTFIKDVIRLDFINFKFSIFTILIFSFVLIGIQVLVTELLVRNIEKKSL
                     TERLRSQ"
     gene            complement(31674..32465)
                     /locus_tag="KR250_001387"
     CDS             complement(31674..32465)
                     /locus_tag="KR250_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBH0067334.1"
                     /translation="METKQYLIDFYNTYDEDSRLALKHGMVEFLTTMHYIDKYIKSGD
                     CVLEIGAATGRYSHTLARQGYDVDAVELVEHNIEVFHKNTQSNENISITQGNAMDLSV
                     FPDNKYDITLLLGPLYHLYNKEDKQQALHEAIRVTKPGGVVFAAYVISDGCLIDEGFH
                     RGNINVSEYIEKGLIDPQTFAAKSEPKDLFELVRKENIDDLMSAFNVTRLHYVASDGL
                     ALYMREAVDSMDDDAFALYLKYHLATCEREDLVGVTSHAIDIFRK"
     gene            complement(32498..33085)
                     /locus_tag="KR250_001388"
     CDS             complement(32498..33085)
                     /locus_tag="KR250_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBH0067335.1"
                     /translation="MPPKVKITKEMILNTVLDITREVGFEAVNARSIASKLQCSTRPI
                     FTCYENMNELKNEFLAFAYEYYNQYVVNYSNSKSVSPYLLLPLSYIEFAQEETHLFKL
                     LFINDLDLMMEEANDFYKEIDNEKKARLFSESIGIELECAKVIFLDLFLYTHGIAVLT
                     ATKKLTLDRYSAEKMLKNILTAFIRQEKPDWNLSI"
     gene            complement(33262..34653)
                     /locus_tag="KR250_001389"
     CDS             complement(33262..34653)
                     /locus_tag="KR250_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate/H(+) symporter BenE family transporter"
                     /protein_id="HBH0067336.1"
                     /translation="MERLARREQPHIKFGMFKIRIPFIHYRFEKPEAIQGIISSMTSL
                     GTIGLSTQILGLPYEVAWSMAIINSILYCLHVFMGDPVVPGWITASITLTTAYLLKFS
                     MGIERIQALTALQIDLGIIFILMGITGVAGKLVSKIPNSIKGGILMGVGISTIISEFN
                     PKGRFDSYPISITVGILVACFVMFSERFDTLKVKNKFLFHLGEYGVVSAILVSLIVGI
                     FSKEIEIPRFSFDNLVYIVDFKNLINTVSPFGIGFPSVMLFIQGIPMAFMIYIIAFGD
                     FITGENLVLSESENRKDEYIDFNSNRSNVISGIRNIFMAIFSPYIPMCGPLAATLTGS
                     VAQRYKVGKEAMQSVFSGMGTLVWVSAIFICFYPIAQIATPLIPLALSVTLLVQGYLC
                     TKLSMELCNTGVEKGLIGLMGGVIAAKGGTWGLAVGFILYFILIDSKKRKEKDINSIE
                     DCVYEILDKKKSI"
     gene            complement(34843..36141)
                     /locus_tag="KR250_001390"
     CDS             complement(34843..36141)
                     /locus_tag="KR250_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase/BAAT N-terminal
                     domain-containing protein"
                     /protein_id="HBH0067337.1"
                     /translation="MKVNVYNVESFVDERLKIMVSDLIPNTKLKISVKMEFPWCKGEE
                     FSSYGVFCSNEKGEVDLDLVEPIEGTYKADNCMGLIYSLEKSKTEGKNFAENISIDKP
                     IIMNMIFETSIERKEVELKRIFKSEDITTKYISDEFIGKLFYKQNSNNKTILMLGGSD
                     GNLDALDLLAAPLASRGFNVLTVAYFALEGLPDKLEEVPLEYFEKVFEWISENEITST
                     KEIFVHGTSKGGELAFLLASRYEQIKKVVVSQPHIYCFQALNGLMSGNDTSSWSYKGE
                     PFPYIKVDNDIFFEEQKKNISKDIPFGFNSTYKKSLKRAENRKEARIKIENSKADILM
                     IAGKEDNIWNSYEACLEALEIFEENNYPYNVELLTYNNMGYPLPIPYIMPLKETLCMS
                     MSRGIFSSGGTVEGNSKGQFESWNRTIEFYKKPLSSDLNN"
     gene            complement(36498..37373)
                     /locus_tag="KR250_001391"
     CDS             complement(36498..37373)
                     /locus_tag="KR250_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HBH0067338.1"
                     /translation="MITFDVNGAIIRFDDTKNNYNKIRKIFKLYALEISKNFERDCLN
                     SFQNLKQISDRGLSLGEKYIEESFKKGIETIVSFGIITIDIDTFKSVYCEKYLDFKRL
                     FNNLNKTFIIPNKNKKNGHASFLEIESTIKKMSNYLYNDCFKIHYAVVDALIENRITA
                     VHSYIDEESIKKSNALFNNYKDGFIGKADECKVVKQIITLNPYREDVYEFLVKEDGDF
                     SKEIERLTEFLGYDIREYKASLMDIYINELMESDENDVENAKEKIEKYARYIGCSDEN
                     LYVARVNAIYTFANA"
     gene            complement(37566..38252)
                     /locus_tag="KR250_001392"
     CDS             complement(37566..38252)
                     /locus_tag="KR250_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HBH0067339.1"
                     /translation="MFGVIANSLAIAGGCIVGLIIKGGLPQRVSDTIMNGIALCVLYI
                     GISGALEGKNTLVTIISVAVGALIGELIDIDKWVNKLGAFLQSKFSKGNKKDSIAEGF
                     ISSSLLFCIGAMAVVGSLESGLTGSHDTLFVKSVIDGIASIIFTASLGIGVMFSAISV
                     FLYEGIICVGASFLNGFLSDPVVTEMTAAGSLLIIGLGLNVLKLTNIKVANLLPAIFI
                     PILFGVFGII"
     gene            complement(38301..38834)
                     /locus_tag="KR250_001393"
     CDS             complement(38301..38834)
                     /locus_tag="KR250_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HBH0067340.1"
                     /translation="MSDLNNKKEQDENESSMNETKIYDILDKLNVEYDVVEHEAVYTA
                     EQLVILDGITKGCQCKNLFLRNAKGNKYYLIVVKGEKQVDLNSLKDKIGSSRLSFASP
                     ERLYNVLKLLPGSVNPFSLINDTERKVELYIDKDVIKEDYLNFHPNINTKTVNISRKG
                     FEEFLEYLKYPIQSIDV"
     gene            complement(38878..39510)
                     /locus_tag="KR250_001394"
     CDS             complement(38878..39510)
                     /locus_tag="KR250_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HBH0067341.1"
                     /translation="MKVFDLTHVTHNDMPVYAEPNRPDIKKVAIIEENGYQETLISVF
                     SHNGTHMDSPRHMYTKGETLDKLDIENFVGKAYVLELEKGNENIELEYLKKYEDEIKN
                     SDFIIFKSGWSKFWDKKQYYVGYPTLTKEAANYIANTNIKGIGIDMLSVDRYDTSVFE
                     VHHILFEKGKIIIENLTNLENVPEKFLFIAAPFKYNDADGAPVRAIAIVE"
     gene            complement(39600..40679)
                     /locus_tag="KR250_001395"
     CDS             complement(39600..40679)
                     /locus_tag="KR250_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HBH0067342.1"
                     /translation="MKIAIFLGAGASAAENLPIQNELFSEYFKKLNPTNYNTKMNREL
                     YIFFKQMFNIDIVKDNIDNANFPTFEEVLGLLDLAEQRKEAFRNFGLENLNNRSDSIR
                     FLRQYLILLMAEAIHNTPRTDNKYHKLLVENLLENDLLLDTTFISANYDIHIDNTVTG
                     LRDKNDLPIMLDYGVEFTNFKEELNWRKPKEPLIKLYKIHGSLNWLYCPICNSVTLTP
                     HEGGVMKLIENSSETKCLECGELTEPIIVPPTYFKNMSNIFLSNVWNETEKTLRDTDL
                     LIFCGYSFPEADMHIKYMLKRVQTNRKKPPLKIMVFNNHSQKQRITLKKEEGRYKRFL
                     GEDVIFTDNSFQDFSVNPLRFIKNI"
     gene            complement(40812..42158)
                     /gene="pepV"
                     /locus_tag="KR250_001396"
     CDS             complement(40812..42158)
                     /gene="pepV"
                     /locus_tag="KR250_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HBH0067343.1"
                     /translation="MKEQIKEKVNSLQDEMISSIQESVKIPSVISEATENCPFGENVD
                     KALRGILDLCKSLGFKTVYKDGYYGYAEIGQGEKMIGILGHVDVVPEGDLESWNYPPF
                     EAVLEDGKLYGRGTQDDKGPTISAIYAVKALMDLNVDFNKRIRFIFGADEENLWRCIN
                     KYKENNEEIPNYGFTPDSRFPITNAEKGLLQVHLTCDSKSDIELSVGKALNAVPGKAI
                     YIGKYSDKLKKELDKLNFEYTVEGNKICIIGKSVHSAASDTGINAVARLCIALNNIGI
                     DSNIIKFLAEVIGEDANGNNIIPNCKDDVSGKLTVNIGRVTIDNEKEFAGIDVRIPVT
                     YKKDDFVKELKKMTDKYNLNYEEYDFLDSIYVPEDTLLVKTLRKVYEEETGLDGTPLS
                     SGGATYARALDNCVAFGAIFPGKPETEHQANEYLIVEDIIKATQIYALSIYELLKI"
     gene            complement(42227..43507)
                     /locus_tag="KR250_001397"
     CDS             complement(42227..43507)
                     /locus_tag="KR250_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine gamma-lyase family protein"
                     /protein_id="HBH0067344.1"
                     /translation="MLNETKELLKDYYGIDDDTFKLSQEIMEEIKDKFEEIKEIREYN
                     QYKVLKAMQESKLSDMHFNWTTGYGYNDIGREKIEEIYSKVFNTEDALVRPIIVNGTH
                     ALTLCIQGIVRPGDEILSVTGRPYDTLEGVIGIREEKGSLKEYGVTYDDVDFLEDGNL
                     DLEGIKNKINDRTKLVMIQRSKGYSWRKSLSISDIKEAIEVIKSVKPEAIVMVDNCYG
                     EFLDTKEPTDVGADVMAGSLIKNPGGGLALTGGYIAGRKDLIELISYRMTSPGIGKEC
                     GLTFGTTRNVLQGFFLAPYIVSQAVMGAIFCSRAFEKLGYDVLPKYDDLRSDIIQCIR
                     LNNADEVISFCEGIQEAAPVDSYVKPVPWDMPGYESEVIMAAGAFIQGSSIELSADAP
                     IRPPYNVYFQGGLTFDHSKMGTLKAIEFIKKLKK"
     gene            complement(43634..43894)
                     /gene="hfq"
                     /locus_tag="KR250_001398"
     CDS             complement(43634..43894)
                     /gene="hfq"
                     /locus_tag="KR250_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HBH0067345.1"
                     /translation="MKNTVLNLQDLFLNNARKERIPVTIYLVNGVQVKGLVKGFDSYI
                     ILIEGDNRQQNMIYKHAVSTIQPGKYINLTNQNQNNNNNNNR"
     gene            complement(44008..44943)
                     /gene="miaA"
                     /locus_tag="KR250_001399"
     CDS             complement(44008..44943)
                     /gene="miaA"
                     /locus_tag="KR250_001399"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HBH0067346.1"
                     /translation="MKKIPLIILTGPTAVGKTDLSIKLAKDMNAEIISADSMQIYEYM
                     DIGSAKVTEEEMQGVKHYLIDEVKPDYPFSVSEFQQRAKKYIHEINEKEKCVLVTGGT
                     GLYLNSLIYNMDFAQSDANNELREELQKQLAEKGIDYMHNKLKELDEESANRIHKNNT
                     KRVIRALEVCLSGKKMNDFSSDLKFNEEYQPIIIVLNRDREHLYQRINMRVDIMIKNG
                     LVEEAKKLLSMGFKKDMISMQGIGYKEILKYLDGEYTYEEAIEIIKRDSRRYAKRQIT
                     WFKRYKTAKWFDLDQYENIDELKNEIILYIKDSIK"
     gene            complement(44981..46948)
                     /gene="mutL"
                     /locus_tag="KR250_001400"
     CDS             complement(44981..46948)
                     /gene="mutL"
                     /locus_tag="KR250_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HBH0067347.1"
                     /translation="MKNIINILDDLTINKIAAGEVVERPSSVVKELIENSIDAGANKI
                     SIDIIDGGKSLIKITDNGIGIPSSEVEKSFLRHATSKIKKIDDLYDLYSLGFRGEALA
                     SISAVSKLEMTTKTKDEIIGTKIYVEGGKIISKEPIGSTNGTTIIIKDIFFNTPARQK
                     FLKSTHAETINISDLINKLAIGNPNIQFKYTNNNKQMLNTPGDGKLVNTIRSIYGKEI
                     TENIIDVEFKCNHFKMNGYIGNNNIYRSNKNLQHIYINKRFVKSKIIIDAITESYKSI
                     IPIGKHAVCFLNIEVDPSCIDVNIHPNKLEIKFEKEQEVYIELRDFLKVKLIHSNLIG
                     KYATYSDKKTQPRIAINSREKSTDYKLRNNDLLESTPKNSNITKGKDEVIEVVTLSSE
                     KPINEFQSVSEVLNASVEDDVKNINYLSEDSANDNIQEEFQVDGIKNEGNYYLGDSIK
                     DSEEEYLCSSKRKFSLYGYFVIGVVFNTYIILSKDDSMYLLDQHAAHERILYERYMEK
                     FYRQDINMQILLDPVVIEVSNIDMLQIENNLELFMKFGFELEIFGNNHIMVRCVPTIF
                     GVPETEKFILQIIDNIEEITSNYDLKGERFASMACRSAIKANDKIYDIEIKSLLEQLE
                     KCENPFTCPHGRPIMVEISKTEIEKMFKRIM"
     gene            complement(46959..49802)
                     /gene="mutS"
                     /locus_tag="KR250_001401"
     CDS             complement(46959..49802)
                     /gene="mutS"
                     /locus_tag="KR250_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HBH0067348.1"
                     /translation="MQIDMNKLTPMMKQYLEVKNRYKDCILFFRLGDFYEMFFEDALV
                     ASKALEIALTGKACGLEERAPMCGVPFHSANSYISKLVENGYKVAIGEQMEDPSTAKG
                     IVRREVIRVITPGTVLDGNLLENKKNNYLLSLYKDGTNIGLTYVDISTGETNATCLNE
                     DKVIEEIAKIHPTEIIINDLDFIEKLRDIATVSNIYINESFSDNYLDINILKEYFPDV
                     YLQKLKFDDKGLIKSSLSILLNYIYNTQKQITSNINNINIYNSSEYMVLDMFTRTNLE
                     LTQTIRGNKKKGSLLHVLDKTSTAMGGRLLRKYVEEPLINKSKIENRLDVIEEIKDDF
                     ILREDLNDILKNIYDIERICGKIAFERVTPKELIHLKNSIEKLPNLKDTINLSNAKIL
                     KEYVSEMDKLDDIYNLIDEAILEEPTITIKDGNIIKSDFSDELKELREISKNGAFLVK
                     EIENREREKTGVKSLKIGFNKVFGYYIEITKANFKQAKLDETYIRKQTLSNAERYITP
                     ELKEIEEKILHAEEKIKSLEYEIFVEIRDTIYKNIDRIQKVAKTIANIDVFVSLATVA
                     HINNYVKPAINENNKLDIRNGRHPVVENIVGEENFVPNDTYLNRGENIINIITGPNMS
                     GKSTYMRQTAIIALMAHIGSFVPAESADIPILDRIFTRVGASDDLSQGQSTFMVEMNE
                     VSLILKNATERSLVILDEIGRGTSTYDGISLAWSIVEYIQKNIRCKTLFATHYHELTD
                     LEEEFKEVKNYSIAVKEDGEGIIFLRKIIPQGADKSYGIYVAKLAKLPDEVIERAKYI
                     LKDLEKNHVYNSVAINGDKENNNIINSNLDEELVKVNQSNFKNKYESLKIEHELIVKD
                     YKHIKKDYDKLNSKFKALNDEVALMKQNDDKEKINQEDSVKEVALTQISFDSVNRDIL
                     SEEILNLDILNMTPLDAINSLYNLQRKAKEIKS"
     gene            complement(49963..50697)
                     /locus_tag="KR250_001402"
     CDS             complement(49963..50697)
                     /locus_tag="KR250_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0067349.1"
                     /translation="MLTQMNFYHNKKVKVSIENVSKTYKDIEVLKDISIDVYEGEFVS
                     ILGPSGCGKSTIFNIITKLTDYDSGKVNINGKYSYMYQKDLLLPYKTIIDNVSLPLIL
                     KKEKKSKARSMVKPYFEVFGLSGYEDKYPSELSGGMRQRANFLRTFINSNDIMLLDEP
                     FGALDSLTKTSMQKWLLDVKKKVNSTILLITHDIDEAIMLSNRIYVISKKPSIVKKEF
                     VIDSRKLNEDNLENIIKLKKEIISLL"
     gene            complement(50666..51679)
                     /locus_tag="KR250_001403"
     CDS             complement(50666..51679)
                     /locus_tag="KR250_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBH0067350.1"
                     /translation="MNFKKLGILGLVSVFTLATITGCSSNNNKDKNNDKDTSSKKVTM
                     VLDWTPNTNHTGLFVALDKGYYKEEGLDVEIVQPPESGAETLVATGKADFGISYQEQV
                     TYAKTSEDPLPIKAVATVIQHNTSGFASPKEKNITTAKDFEGKTYGGWGSPSEEAVFK
                     AVMKKNGADFNKLKIVNTGQDDFFAAMKTVDFAWIFEGWDAVKADLIGYDLNFIPVKD
                     LDERLDYYTPLIISNETVLKNNPELAKKFLKATTKGYEYAIKNPEESAKILVKHAPEV
                     DEKLALKSQEYLASKYKDDAPRWGEMKDSVWNNYTSFLKEYKLIDKDMKASDAYTNEF
                     LPQ"
     gene            complement(51701..52471)
                     /locus_tag="KR250_001404"
     CDS             complement(51701..52471)
                     /locus_tag="KR250_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0067351.1"
                     /translation="MKNLEIIDKVKDSIYPIATFLFILILWQSMVGVLEVPQYILPTP
                     VDIINVFFKDYQNLSIHAVVTIGEAILGFIVAIIISLVIGILMDFVSIIKKCLYPIML
                     VTQMIPTIIIAPLFMIWFGFGTMPKVLMVMLTCFFPILISFVDGIENIDKDYLNLFKT
                     MDSNKVNTFIHLKFPMAMDKFFSGLKISATYAVMAATVAEWLGGTKGLGVYMLRSKSA
                     YALDKVFASTILVVIFSLMFVGIVQAVKKVVIRHRLID"
     gene            complement(52446..52736)
                     /locus_tag="KR250_001405"
     CDS             complement(52446..52736)
                     /locus_tag="KR250_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HBH0067352.1"
                     /translation="MVIADIAVMPLRPYKGEEEMYKVVDACIAEIEKSGLKCEIGAMS
                     TTVEGDFDEVFELLKKVHKIPFNLGCERVITVARVDEKAGGLTIDEKLRNHR"
     regulatory      complement(52867..52966)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
BASE COUNT        18176 a         8490 c         6021 g        20840 t
ORIGIN      
        1 aagattatct tttttatagc tatgacttac atatcctagt ataccatcat ttttactttc
       61 tattggtctg aaaaacttaa ctgaatttat tttatgcatg aaaattaaac acattatcaa
      121 atgactcaat tcatgaacaa acgttcctat agctccagtt acaataattc cagtttttcc
      181 aaaagatgaa aatataaatt tattatttag attttctgta atgctaaaaa tcaatccaaa
      241 tacaataaat attcctatga aacaaaaaat atttattaca cttattttta aaatttcgat
      301 tatataaatc ataagttaag tcctcatgta aaagtattta actactctac ttcatatcca
      361 ttacagctta aaattttaca agcattttct atatcttttt cttttactaa gatatagtct
      421 gtatcataag tagaaattgc aaatatgctt attttgttgt ctgctaacaa actacttaac
      481 ttagataaaa ttcctacaag tgaaaagtct aaaggaccta gtatcttaag tattctccac
      541 tccttttctg acttgacatc agaaggaata ttattattta aacatacaac acttaattca
      601 tcatcagttc tagttataga gtaaaacttc tctgtatcta tccatgttgg tattttactg
      661 tcattattta atctacatac tgcatactct tctaccaata attttaattt cataatgtat
      721 atttcctcca atcttatttg tttacttaag attatacatt aaaagttaca taaaataaaa
      781 catataaagt aaaaaaggtt cactatagtg tcctatttct atagccaacc tttaaaactt
      841 aattaaattc taataaagag atatattatc taggttcaat tataagttta atggctgttt
      901 tcttatcacc ttcgatttct atatcagtaa atgcgggaat gcaaactaaa tccattccac
      961 taggtgcaac aaaacctctt gctattgcaa ttgattttat cgcttgattt agagctccag
     1021 ctcctatagc ttgtatttct gcaacgcctc gttctcttag tactccagcc aaggctccag
     1081 ctacagaatt aggattagat tttgatgaaa cttttagtac ctccatttca acatctcctt
     1141 gttaatttgt taaagtatcc attacctaaa gtactaaatc ataattgcca tttactaatt
     1201 acaatcatca tttaaagtat acttttataa aaatatactt ctataatcgt tttaagaaaa
     1261 cctttatttt ttttaaccat tttattatga aaaaactaca aatttcttta tattctataa
     1321 gcattttcca atatttctaa attttttata tctttgagct ataatttcct ctttatccat
     1381 atctttgtaa tgatttaatc tttctaaaat ataagctttt atttcttttg aaacagcttc
     1441 tacatctttt tgtgctcctc caagaggttc ttttataatc ttatctatta tttttaaatc
     1501 atataaatca ttacttgtaa gcttcataac atcacaagct tctttagctt tagatgaatc
     1561 tttaaataga atactagaaa gtccttctgg agaaattaca gaataaactg aatgttctaa
     1621 catacataca tcatttccta ctccgatacc taaagctcct ccactaccac cttcaccaat
     1681 tacaaaagtg attactggaa cagaaagttt actcatttca agtaagtttc ttgcaatagc
     1741 ttctccttgt cctctttctt ctgcttctag accacaaaaa gctcctgatg tatcaataaa
     1801 cgttattata ggtctattaa atttttcagc ttgtttcata agtcttaatg cttttctgta
     1861 tccttctgga tgaggcattc caaaatttct ttttatattt tcttttgtat ctcttccttt
     1921 ttggtgtcca actatagtaa cattaaactt tcctatctga ccaattcctc caaccataga
     1981 agggtcatct ttaaaaagcc tatctccatg aagttctaaa aaattagaac aaatatgttc
     2041 tatgtattcc aatgtcgtag gtctctttat atctctactt aatgttacct tttcatatgc
     2101 tgatagatgt gaaaaagcat cttccttaag cttttccaat ttctcattaa gtgaatttat
     2161 tttatcactt aaatctatat tatttgcctt agaaatagtt attaattgtt taatatcatt
     2221 ttctaagact tttatttttt cactattatt ttcaatcaaa acagttcctc ctttatgccc
     2281 tatttcttgt gcatagcaag aatttgatat aatacttcct tcatttttct tctatctact
     2341 atcatgtcaa taaaaccttt ttcaagtaaa aactcagaag tttgaaatcc ttcaggaagt
     2401 ttttgattta ttgtttgctc tattactctt ggacctgcaa aacctatcaa cgtatttggc
     2461 tcagaaatta taatatcacc aagcatagca aaactagcag ttactcctcc agtagtaggg
     2521 tcagttagaa ctgatatata tggcaataat ttttcttcaa gcttagaaag tgcttgagaa
     2581 gtctttgcca tctgcataag agataccata ccttcttgca ttctagctcc tcctgaagca
     2641 gaacaaataa ttataggtaa attattttca gcagcatact ctattgaata agttattttt
     2701 tccccaacaa tagaacccat acttcccatc ataaaatcag gattcattac acaaataact
     2761 gttttaattc catttattct tccataacct gtgacaacag cttctgactc ttttgttttt
     2821 tccttataag agtctacttt ttctttgtat ttaggaaaat ttagtggatt ttgaaattct
     2881 acatcagaat taaactccat aaaagttcct ttatcaatca ataaatttat tctttttctt
     2941 gctcttaagc cataatgtct tgaacatttt ggacatacaa atgaattttc ttctatgtcc
     3001 tttctaaata catgactatc acaacctttg caataggtcc aaaatttatc atctacacta
     3061 ttctttttaa agttgtcatc taaagaaatc gtaacatact tagattcttt tgatgataaa
     3121 aattttttta tcataatcta tttactcctt ataatgtagc aaattctttc ttcataaaga
     3181 catcttctat aaatttggta tgatgagtat cttctctaaa ctcctcactt gttacaagtg
     3241 ccttttgaaa ttctacattt gtattaatac cttctacaat aatttcgtct agagctctat
     3301 atattctact tatacattct tctctagttt cagcccaaca tattaactta cctatcatag
     3361 aatcatataa aggtggtata gtatatcctg catatacaaa agtatcaaat ctaacaccaa
     3421 aacctccagg taaatttaat gattctatct tacctggtga tggagcaaaa ttctttgatg
     3481 aatcttctgc atttattcta cattcaatag catgacctct aaaagtaata tcttcttgtt
     3541 taaaaggaat tttatcccca ttagctatag atatttgaag ttttataagg tcaagatttg
     3601 ttaccatctc tgtaactgga tgttcaactt gtattcttgt attcatctct ataaaataaa
     3661 agtttttatc tttgtcaact ataaactcaa tagtacctgc accgatataa cctactcctt
     3721 ttactatatc tactgcaatt ttatgaagtt tttgtctttc ttcatctgaa aggtatggac
     3781 ttaaagattc ttctattact ttttgatttc gcctttgcat agaacaatct ctatcaccta
     3841 aatgtattgc atttgaatgt ttatctccaa atacttgtac ttctatatgt ctaggatttt
     3901 ctataaactt ttccatatat aaatcactat tagaaaatgc tgctagagct tctgaacttg
     3961 ccatatcaaa atttgaaaaa agttcttctt cttttctaac gattctcata ccttttccac
     4021 cgcctccact tgaggctttt atcataacag gatatccaat ttctcttgca acttctaaag
     4081 cttcttcctt agttttcaaa acagattctg aaccaggaac aacagggata ttcaattcca
     4141 tcatagtttc tcttgctcta gatttatctc ccatcaaaat catttgttct cttgttggac
     4201 ctataaaaat aatattatta tcttcacatt gctttgcaaa ttcaggattc tctgataaaa
     4261 atccaaagcc tggatgaata gcatcacact ttaatttaag agcaagatag attatatttt
     4321 ctatattatt ataactctta cttatattat ttggtcctat acaaatagat tcatcagcta
     4381 tatatctatg aaaacaatct ttatctattt cagaatagat agcaacagtt tttataccta
     4441 gttctttaca tgttctgatg attctaactg ctatatcgcc tctatttgca actagtattt
     4501 tttttatcaa cttaaatacc tcctactaca atggttttat cttaaataaa ggttgattat
     4561 attctactag ttcttcatta ctaactaata cttcaattat ttcaccttta atttcacttg
     4621 ttatctcatt cataagcttc attgcttcta atatacataa agtgtcgcct tcttcaacta
     4681 catcgccaac tttaacaaag ctatctgcat ctggacttgg agaatcatag aatgttccca
     4741 tcaaaggagc tactactact gatagattct cattagatgt gacaggtgta tcgtttacaa
     4801 ctggcttttc taaagaaaga tccactactg attcttgttg tacacttaaa acaggtgaac
     4861 ttgtagattg agctttttta gatacttcta atcttaaatc gctactttca agttttacat
     4921 attctaagtt agttgagtct atggccttta ataattcctt aatttcattg atgttcattt
     4981 tgttaccccc ttgtattatt acctggtgct aatattatat cacaaaaaac aaatttataa
     5041 aagaaaaagt gctgactaga gaatcaacac atcaatatat cttttaattt taaattaaca
     5101 accagtaact tatatttgtt ttaattttcc attattaata taaattataa gtataaaatg
     5161 aataaaattc aatatttttt gcaaaataca agagaataca aattaatacc tcgggaattt
     5221 ttaagacaaa taccaatatt atgcaattta gacttcgctt tctttcaaat tagtatcatt
     5281 ttctttattt ttacatagga tgtactttaa tttagctatc aaaacattgc tatataggtt
     5341 aaaagttatt agaattgcag gaattataaa gtaaactcta gcacctataa aaccaaaaac
     5401 tactgtaata agtattaatc ttttatttaa agtaaaaaaa ggtatattaa aaatactttt
     5461 gtcggatgtt tctggagtat aagaatatat aactccaaaa atcaaagtaa gcatacaaag
     5521 tggaagtagt gtataacttg aaattattcc cagaaatcct atactttcaa aatatttgct
     5581 agcactattt gtataaacaa acatgtatcc atttaaatat gcgcatatac atgatataat
     5641 gccaaaagtt ccccctaagg attgagtgta ccaattaaaa aatttagatt ccatatacct
     5701 cacctcctaa aactgtatat ataatgatat gcatttaaat agtaaaatat atctttatat
     5761 gtttctgaat aaaattttgt taatttcatt aatttatgtt taagaaattt gaagtatagt
     5821 gtagtatata ttaatgtaga tgaagtttta tagtacttat ttcatagtgc ttaatcaagt
     5881 tatcttatta tggattcaaa agttgtttgt agggttttat attttgttga gctgaaaaat
     5941 ttattataaa tgaagggatg ttttcgcttt ggtagataca ttagaatttg gtcttaaaat
     6001 tttatttttt atattatcaa taatttggat gggtaaaata atgattttaa gaactgataa
     6061 gcaaatagta atcaatcctt tgcttattgg tatatctgca gtccttgtga tgttgcatac
     6121 ctcacagagt aacattgaat tttttggatt agatgtccaa tatataagaa tagttttgta
     6181 tataatatat tcactgataa ttttaatagg aatatgggct actaacagaa gaaatggaat
     6241 tttttagata atctatttct ataaaattta aaggttgcta gtaacaaagc actaatatta
     6301 gcgttctgtt actaacaacc tttattttga ttatacactt aaattgaaaa tagaatgatt
     6361 gaataagttg acagattatt ttacattgac agtaattaga acttcttgag atgtatttaa
     6421 atcatttttg tcgcttagta taacttttac cactgcttga cctgttttat tacctttaat
     6481 tatcatatct tcacatgaaa gtacactctt atcacttaca gatacaataa aatttaaatc
     6541 ttttggaagg ttatcagtaa cttcccagta tttataataa ctatcaattt tcttcgtaat
     6601 tatttttctc aaatcaatac tttcaccttt tgctatatca acgttatgat atttagagtc
     6661 cagtttatac tgattatctt ttgtatcaat aaaatttaaa tcgcaattta catccacacc
     6721 attatgagaa agagaattta ctacatcgtc atcaatcaaa taattattgg caagatttat
     6781 tgaatccata tcttttagac ttaaagcttc cttaggaatc tcttttatat tattattcca
     6841 aaaaataaaa ttgtctacat tttcaagata tgatatacct tttaaatttg aaatgctctc
     6901 accagttgca taaaattctc catctaaagc ctttatattg cttatagtca tctcatctgg
     6961 atttatatat ctttttacaa ctgctcttaa atttgcatca ggaaaaatat ctttaagttg
     7021 attttcgctc aaaaccttat catttgaatt tattggactt aaagctgctg cagaaataat
     7081 aaatggtatt aaattagcta aattcatagt accctcccac ataaattttt tattataata
     7141 atttaatttc tatgagaggg ttctgtttcc ttgttatata tacctacttt cgtacacttt
     7201 attaatataa attaaaataa atttctacta ttaaacttat aaacatatta ctagttacgt
     7261 tttttagctt cacatctaaa tattcgcata ccttgtgaca taaatttgtc ttcatactct
     7321 gttgtaacat tattttcata ctcactattt cctaaatcaa gagaaatatt ctttaatagc
     7381 caattatttg cagcaatttc atttaaacta aactcgaata atttttcatt atctgtttta
     7441 aagtgaattt cgccatcatc ttccaaaacc ctattgtaca tttctaaaaa tccagaatga
     7501 gtgagtcttc ttttactcca tctattttta ggccatgggt cacagaaatt tatataaatt
     7561 ctcgataatt ctttagcttc aaagtaatcc aaaatattgc ttacatctcc ccataaaaat
     7621 agtatattat tcaaattaga agcatctgct ttttcaacag cctttaaaag aacttcttcc
     7681 ttaagttcca tggcaatata atttatgtca gggttctgct tagctaaagt tgttataaac
     7741 ttaccttttc cagtcccaaa ttcaacatgt attggattat catttctaaa ctttaaattc
     7801 cacttgcctt ttaatttgtc tatatcgtct cttaaaacat attttgtata acttaaaagc
     7861 ttttcatctg cacctttttt tcgtcttctt ctcacaataa ttctccttac atataaaaaa
     7921 ttagtaaaat acatttttat tatacattat ctcaccaaaa aataaaaaga actctcttaa
     7981 aatagaaatt tatttctata aagagagttc taaacttact aaaaacttaa tcttagtggc
     8041 accctgaaca accagagcat cctcctccac cttgatcttt catattttca ggaataactc
     8101 taaatccaaa tcctgtatct tctattatta tatctccata ctgactatat tcatcagagc
     8161 tcattacaaa ttgtaattct ccaacttcat aagttaagtc atcttctttt ttctcatcta
     8221 aagctaatcc aaaagaagga cctccacaac caacacctgc aaagtatact cttatattat
     8281 taggtttatc ttgattgtct tttaagatac tttttaaagt atcaatagct gtttctggta
     8341 aagtaatttt catcaaatca ttccctttca aatattttat taaaacaata tctattatat
     8401 acccatacaa ggtacataat caattagttt ttgcatattt tactaaaact tctacctaat
     8461 attacctaag gctttaaaat attttatata atattttatt accttaaata aataccctat
     8521 cataaactta gtaaacaaat aaaattatta ttctaaaaaa tataataatt ttatttgttg
     8581 ttttaatatt ataacacatt tgataatgtc caattcatat atgcaaaatt tatatacttt
     8641 aaaatatcta agaaattatt caataaaaaa atactatttt ttaaaaatta tcattattga
     8701 taaagctcta ctatttatat tttttagtgt attcatgtat aataagtaaa agcacaacgg
     8761 tatatactgt tgtgcttttt tgtaattaat aaacaatatt ttggtctata acaatgaaaa
     8821 atggctactt tccataattg atttcatctg acataagcaa aaagggtgtc cagctgggtc
     8881 aatcataact ctccagtcat cagtaaattg ctcctctgcg attgtagctc cacaatgaat
     8941 tgcatattga actgcttctt ctaaatcatt aacagcgaaa tccatatgag ccatttgctg
     9001 ttgagattca ggtttttctg gttctattat gccccaagcc tttgtttttt gcaatataaa
     9061 gaaaaaatta tatattttaa tattttataa ttgttgaaat tgtgtaataa attcaactgg
     9121 atacatagtg cgttttgaaa tttcttctaa agctaaacct tgttttaaaa gcctctttat
     9181 gacgctttcc atactttctg aaatttctat aatattttta tcaaaatcat aaaacctaaa
     9241 agttctttga ccccatgggt attcttttat ataatgcaaa aattcaatat ttcctattct
     9301 atttatctta ctttcccatt gtttgatgtc attaacctca aaatacaact gaaagttatt
     9361 tgattgttga tatattggta aatcatatcc aattatagct ttataattac tttgtaatcc
     9421 aaatccattt tcaaatgata catgttctcc taaatctaat gtgatttttt gctccataac
     9481 atcttcataa aacttttttg atttatttat gtctttgaca attagcaata ctcctccaaa
     9541 tttcatctta tcactatcct ttctttgtta taagtattat aacttaaaat tttgcaattt
     9601 cactgtaaaa atcagacata ttagctcggt atttttgagg agttattcca cagacaattt
     9661 taaaatcttt aataaaatgt ggtatatcat agaatcctaa ttcattacat atagacatca
     9721 atgaagtttt attattgttt agaagtttta tagatttatt aatacgtacc aaacgagaaa
     9781 atgacttcat actcaaccct aggtactgat taaagagtct gtttaaatgc ctagaactat
     9841 aaaatacttt tttagaaatt tcttgtgaag atatatttcc agagttttga ataatcaact
     9901 gaattgccaa aataaactca tatgagtatt caattttaat attagaaatc aataattctt
     9961 ccaactttaa aatcaattta tctatagata aagattcatt aaatattttt ttaatttcta
    10021 aatctaatga agtattaatt atagaaaatg gaacaacttt atcaataagt tcactttgct
    10081 gtatacctat aagcgggaaa aaaccagcag gttgaaattc tataattagt ataacatcac
    10141 atctattagc aatatttcct actgtttttg gtttagtagt tggaccaaat aatagactat
    10201 gtaaccctgt ggcatcataa aaaaacacca aagttacact tccatgagga ataatcgtgt
    10261 aattatcaga aattattgtt tgatttggaa acgatatagt aaaattcgat atccaatttc
    10321 ttaattgtat acaaggcaag agataaacaa aattgtcttc acaaactata agtgacctat
    10381 ttttatttat aaaatgaata ctttcttttt ttaacatata ttatatcctg tcctatcgtg
    10441 aatatttatt aaagtaagaa gaatccatat tatctctata tatcattaac tgcttttaat
    10501 aatagagata gtatgaatac tcattagata tttaataaac aaataaaatt aaaataatca
    10561 ataaaaaata tagtactttt atctgttatt ttaatattat agcacattta tagtatttaa
    10621 cttatatcca ctttacatat gtctaattta tataattaaa aatgcttatc tcaaatacaa
    10681 taattagtcc ttatacaatt aaaccatctg aaactgtaat agtacgacta ctacttttgg
    10741 ctgcttctgg tgagtgagtt accataatta tagtttgtcc attgtctctg tttattttat
    10801 tcagtaaatc tattatttct atccctgttt tactatctaa atttccagta ggctcatcag
    10861 caaatagaat ctctggtttt ccaattaaag ctcgagctat agcaacacgt tgttgctgac
    10921 caccagataa ctctcgtggt gtatgtttac gtctatcagt taaaccaact atatctaaga
    10981 tttcatctaa ttgattttta taatctttta aatttttacc atctaataac aaaggaagca
    11041 ttatattttc ctccacattt aaatttggta ttaagttata aaattgaaat acgaatccta
    11101 tatttctacg acgtattatg ctcattttct catcattaaa atgtgatata tcagctccat
    11161 tcatatacac ttttccgctc gttggagtat ctaaaccacc taaaatatat aaaagagtac
    11221 ttttgccaga accagattga cccatgatag aaacaaattc acctttcatt acttgtaaag
    11281 atacctcttt taacactcga gttgttgtat ttccaatttt atattctttg ataatatttt
    11341 ttgcttctac agctaaacta ttatgtgtaa ttttcatatt aacctccata attattcaaa
    11401 cttaatttct tctatcagtt tcattttctt cccttttata ataggcacta tcgaacctat
    11461 taaagttact ataattccca ataacccaac aataataaat gttttaaaat ctaaatctgg
    11521 tttcattgaa atttttggtc ctgcaactat aaagatagtt tgaatctcaa gtatagatat
    11581 cacaatacca ataacagctc caagtaaacc tgaagtaaac ccttcaatca atgtcacttt
    11641 tatattttgt ttattactta gtcctataga tttatacata gctatagaac gtcttttttg
    11701 aatataatta atcagtagat tattaataat acctactgta gcaagaagaa aaatgaaata
    11761 ggtcatctta ttcataggag acaaaaaact acttatagta tttaaagaat cattattaaa
    11821 ttcttcaact gtacgactcc aattatatgt atctccaaat aaatatctta tctgaatcat
    11881 aacagcatct ggattgactg ctgtatatac taaaaaacca taattagttt tatcaaaatc
    11941 attaactgca taatgtgatg gtataacagc ttctacatca tttgcgcggg atttaaaact
    12001 acctactatt ttataagaaa aatcatactt atcattagat agagttattg tatctcctat
    12061 agataagcct acttttttta gagtatcttc attcaaaata acagaacgtt tatcattaaa
    12121 agcttctatt acttgttttt ttatctcaaa atttgtataa ttgattccaa acatagagtt
    12181 atatagtttt atattatctg taccctctag tcttgataat gttacaccat taccagaaat
    12241 ctcattattc ataacataaa gaggtaatat ttttttaatt ccatccatat gtctaacatc
    12301 ttcaataaac tcttcattca tctttccatc tgcaaaacct tgtaattctg cgtcacgaaa
    12361 cacatctgtt atataagttt tgacaaagtt accaacaaca cttattgcaa taactgcaga
    12421 aatacttata aatagtaaag tgatattctg tattatattt ttgttatttt tcatatttct
    12481 agcagctaat ttaccctcat tccctaaaat atttttataa acaaactcaa aaactataga
    12541 catgatatca gtaataagag gaatcaacac aatagtagct acaattaatc ccagtaatga
    12601 aaatccacca gctagataca atgtattttc aggggatatt cttggaagta aaatagataa
    12661 aataaacatt atacttccta taaaaagtat agaacgattt gaaacattct tttcttctac
    12721 tgttcctagt acaatatttt ttatcggcaa atggctagct tttttaacag gtatatatgc
    12781 actaaatgaa gaaacgatta ttgctactat aacagatata atcactccaa tgggtgatat
    12841 tactactgga attgatattc cttgttcgag agaatttcct aacccatgaa gcattaaatt
    12901 taaaacaact acaccaatag gaatagcaat taatcctcct atacttccgt ataatatact
    12961 ttcaagcatc aatatacgag ttacagtttt ttcattggca ccaatacttc taaatgttcc
    13021 aataaaagga agacgttcta atgtaataac cttataactg ctgtatatta taaatatact
    13081 aagggttaat gcaaaaaaac ttattaaaaa gaaaggcatt gtcttttgtc tagcatctga
    13141 aattatctta gtttcattta tagtatttga taccatatat ttttcagtag aaagttcttt
    13201 ggataattta tttactagat tttctgtaat ttcttcttct gactctataa gaactcttgt
    13261 atatccactg cctttattta taatttttga tagagtctct ttgggtagca gtgcatttac
    13321 acctcttgtg ttcctcaaaa atactgtatc ataagatgca atatcactaa cttgaaaagt
    13381 ataagacttt ccataaatct gcaaagtaat agaatcacct ttttttattt tatattttga
    13441 tgtaaagcga tttggaagaa taattttatc accagaaaaa tcagtaatac tatcgccatt
    13501 ttctaatcgt ggcttgttga ttttatttag ttgacttaag tctgctgata taagactaaa
    13561 ttcttcatag tatccccctt tattataaat agcagagctt tccaatacac caacctttga
    13621 ttttatagca tttaaatcag gtatgtcttc taaattggtt aatatatctg gatttttact
    13681 ttggacagat atagtcgctg taccatacat accttttgcc atttttcttt gtgcactttc
    13741 ataagataat ccaattgaga atgaaacaaa aagtaaaact gttgataaaa ctatggacaa
    13801 taacataact gctgttctaa tttttcgttc tttaatattt gtgaaaatat actttaatat
    13861 aattttcaaa tttacacctt ctttccttat ctaagatatt tcaatctaat atttttatac
    13921 tttaatctaa attaaatctc taatagactc ctccatactt ttatcattct atttataata
    13981 tttttaatca aatcacccct taaataaaaa agattgataa actactatat aaatataata
    14041 atctatcaac cttaatatat tttttatttg accttaatta accttaatta aataggaaag
    14101 gtgatataaa atgagctacc taatccaggt atactttcaa cagatatatt ccctccatga
    14161 acttcaataa tttgcttcgt tattgctagt cctaaaccag ttccttctat tttttgtttt
    14221 gtattgcttc ctctataata acgattaaac aaatttgatg cttcttctct tgacatccct
    14281 tttccattat cagatataat gatttcaatt ctttcattta atgttatatg tacacaaact
    14341 ttagtatttt tatctccata tgtaaaagca ttgacaataa gattactaaa agctcgtttt
    14401 aatagtgtac aatcaaaatc aaagttaata ttatctatat tactcttaaa ttcaactata
    14461 tttgattcat attctggata gtttaaaata tcaatggata attctttcaa aaatcttact
    14521 aaatcataat tatttttatt taaagggatt attccatttt ttaattgata ggttaatttt
    14581 aaatcgttta taagtgtatt tgtatattct atattcttta acatgattga agcatattta
    14641 tatacttttt caggtgaata attagtacta ctatctgtaa gaagctctgc ataccctttt
    14701 attggtgaaa gtggagtttt taaatcatga gttatatttg aaatccactc ttctctcata
    14761 atatcagttt tattttgtat ctcatcactt ctatgaattt cttcatttaa aatatttaaa
    14821 ctattatata tatcagcaaa aacaccttta atattaactg gaagatagct tcgttttgaa
    14881 atatcgctaa cagcagatgt aatatttgat atgcactttg ttacccagta accataagca
    14941 attccagaaa ccaaaattat taaaaatata atccctatga ttgaaaatac aattgactta
    15001 ccacctataa atttacctcc atcaaaaaac atattcactt tagatatatt taatggaaaa
    15061 tatataatat aaatatagtc atttttatta tttttaattg tattaaagta tacagttcca
    15121 ttacttgaat tattttggtt agctaatgct aataattcag aattagaata ataatcttta
    15181 tgctcttttg gttctaaaaa cccataaact ctatttccac tagaatctaa aatttgcaac
    15241 cacaattgat tttctcgaag tttttctaat ccagactgtt ttacttgtgg cttattatct
    15301 ataaatacga tttgttcatt aaagttttct gtaaattttt tcggccagtc actacgaata
    15361 aattccccat ttggtttctg aatagtaata agtgataaga aaatacctat tactgtcagg
    15421 attaagcaaa taaaaatcat gaaaaaaata atgtatgaat gaaatactgt tttataacta
    15481 ggtttatgca tttaatcaac tcttttctta aacttttatt ttgattattg cttatataaa
    15541 atagacaata attattgata tgtaaaaatt tatttaaaaa tacaactatc tacataaatt
    15601 atatttatat aattatatgt tatcaagcta tctgttgtat ttaataaatc cttttgataa
    15661 catataaaag taagataaat ttttccatta aataccttat aattatacac aaaatctcta
    15721 tatacgttaa acagtataga gaaaattttc atagaaccat ctttataggg gatactttat
    15781 aaaagaataa aaaaattata atatataaaa acttcatagg agagtaacaa catcagtaaa
    15841 aatatgaaaa cactatataa aaattcaaaa atattaagaa taatgaaata cttgaaattg
    15901 cttatatcaa gaaattttat ataatataaa ctggtcaaat atgttataat attaaattgt
    15961 gacaagttca tggaggtgtt tatatgaaaa aacaaaagtc gccaatttta cttgaaatgc
    16021 ttggactatt ttttggttgt atatcaatga gtattggtat aaatatgttt ttaaaaccac
    16081 atacaattgc tcctggagga cttagtggtc tatcattagt actaaacaag gttacaggac
    16141 ttccagtatc agctatcatg ttaattatag gtgtcccatt agtaatactt gcttttagaa
    16201 ttatgggtac aaaaaattca ttaaaaactt tatttggaac agttgtattt tcagctatag
    16261 ttcaattaac agacccatta tcaaaactaa gatttactaa tgacttatta ttgtcttcta
    16321 ttgcaggagg aatcctagtt ggtataggtt taggaataat gtttaaatca gatgcttcta
    16381 ctggaggaac agacttaatt gcactcatac ttagtaagaa attcccagga ataaaagcta
    16441 caaaattcat gtcatgccta gatggaatgg ttgttatatc ttctggtata gtaaatcgta
    16501 gtttagaaac tgctctttac tctggtatag cactttgtgt attaataaaa atagcagata
    16561 tgataatgga aggatttgac cattcaaaag cattttttat tatctctgat gagcctgaaa
    16621 gtttaagaca agctataact gaagaacttg atagaggtct tactattctt gatggtaaag
    16681 gtggatacac tagagaaaat aaagaagttc tattagtcgt agtttctaaa aaacaagaac
    16741 tttatctaaa gagattagtt aaaagaactg accctactgc atttgttatt gttacagatg
    16801 tacatgaggt tcttggagaa ggatttaaaa atttagagaa ctaataaaaa ttaatatttt
    16861 aagtatatat actattttaa ttaaaatacg atttattcaa aaagagtgtc tcataaactt
    16921 tttagttcat gagacactct tttttgtata aaatcaaatg cattttcagt attttaacaa
    16981 taaaaacagg attatctata ttttgaggca accactttat tgaaatttta tttagaaaac
    17041 aatcattact aaataaccag catagatatt aaatgattat atctattgtt ttttcaattc
    17101 atattgataa caaggaacat ttttcacagt atttccagta tgaatttttt tgatatactt
    17161 tcctgtacat ttaaatccta ataaatctaa tgattttgta gaattttggt tttcttcata
    17221 ggttattgca attattttat ttaagtttag ttctctaaat ccatactgta ttactctttt
    17281 tgttatctca gtagcaaaat catgtcccca atagcttgga tttatacata tacctatttc
    17341 tgcttctaat gctttaggat ttatattatt aaaccccata ttcccaataa ctttattgtc
    17401 tcttttatat actatagcaa agtggccaat tttccctttt ttatatggat ttagaaaaaa
    17461 tgattttata aactttcttg tatcttctaa agatttatgc tcactaaatg gtaaatgttt
    17521 tactactatt ttatttttat aacagtcaaa aaaatcttca acatcatcta atgatgcagg
    17581 tcttagtata caatttctag tttctaaggt tttcatttga ggatattcag aatcagacat
    17641 ttctaatccc cctttataca ttaatctaaa tatactgttc atatttaaaa tacgatatta
    17701 attatataaa tgttctcaaa ttaaccttat ttttattttt aataaaaact aattactata
    17761 tgaatctttc aaatttacga atttatctct atttttatat taaattactt gtgaaatatg
    17821 tattctaaat tatatattat tagatatcat gcttaaaaat tctttggttg ctaaggataa
    17881 agatatatct tttaaatggc agtaacctat atttctctta ggtattggct cttttatatc
    17941 taatttaaac aatttctcat tttccaaata atctatgcta aattcttcta taacacaaga
    18001 cactccaaga tttatatacg caaattctaa aagtaattca tgagaaccca attctatatc
    18061 cgggtttagt tttatacctt ttgataaaaa atattcattt acataaagtc ttgaatttgc
    18121 tttattttct agcattatta aaggcaatgt gtttaattct tccaatgata ttttcctacc
    18181 tttgtattct atgtaatcat ttgcacatac aaatatatca tgtatttcaa tacactcaac
    18241 aatatttagt gtatcatctt ctattggcat gtttataatt gctatatcta tattcccatc
    18301 tttgagtaaa gatataagtt ctctactcgt tctatttatt gtagatacat gtatatgtgg
    18361 atacttctta tgaaaaattt ctaaatattt taacaagaaa aaacgtgcag ctgtatctcc
    18421 tactcctatt ttcagagaac cgtattctaa ttttttaaat ttatttattc tatcttctcc
    18481 tgatgatatt aactttaatg cagtagtgac atgttcactt agaattttcc cctctggagt
    18541 taattttact cccttattgt ttctataaaa taataaagta tctaattgtt cttctaattg
    18601 ctttattgac tggcttacag ctggctgtga cataaacaat tccttagcag caagagaaaa
    18661 actcttatta tttacaactg cattaaaaac cttataaagt tcaagtttta tattcatata
    18721 taattactcc ttatgtcaaa tattagtatt atttatttta cttataatta tatttgaatt
    18781 ataatataca tggtactaat atgacaatat taattcttta ggaggatttt agataatgga
    18841 tagattagtt ggaactgttt caagaggggt tagagcacct attataagac aaggagatga
    18901 cttagttaaa atagttgttg attctgtttt aaatgcgtca aaaagtgaaa actttgaagt
    18961 tagggacaaa gatgtaattg ccgttacaga agctgttgta gctagagcac agggaaatta
    19021 tgctcacgtt gacaatattg caaaagatgt aaaagataaa tttggagatg atactgttgg
    19081 ggttatattc ccaattttaa gtagaaatcg ttttgctata tgtttaaaag gaattgcaaa
    19141 aggatgtaga aaagtcgtat taatgttaag ctacccttct gatgaagttg gaaatcactt
    19201 aataagtatt gatgaacttg atgataaggg aataaatcct tggtcagatg ttttaacaga
    19261 agaaaaatac agagaactat ttggatataa caagcataca tttactggtg tagattatgt
    19321 tgattattat aagaatttaa tcaaggattg tggtgcagag gtagaaatag tatttgcaaa
    19381 caatccaaaa actattctta actatacaac aagtatttta acttgtgata tacatacaag
    19441 acaaagaaca aagagaattt taagacaaaa tggagctaaa aaagtatatt cacttgatga
    19501 tataatgaca gcaagtgtag atggtagtgg ttacaatgac cagtatggac ttttaggttc
    19561 aaacaaagct acagaagaaa cagtaaaatt attccctata aattgtgatg aagttgtaaa
    19621 caaaatccaa ggtaatataa aagaaataac agggaaagat gtagaagtaa tggtttatgg
    19681 agatggagct tttaaagacc ctgtaggtaa aatatgggaa ttagctgacc cagtagtatc
    19741 tcctgcttat acaaaagggc tagagggaac tccaaacgaa gtaaaattaa aatacttagc
    19801 agataatgat tttgctgact tatctggtga tgagttaaaa gaagcaattt caaaatacat
    19861 agttgaaaaa gataataaat ctgatgattt aacaggaaac atggtttctc aaggtactac
    19921 tccaagaaga ttaacagacc ttataggttc tttagcagat ttaacatctg gaagtggaga
    19981 taagggtact cctattattt acatacaagg atatttcgat aactatacaa aatagtatgt
    20041 tttaataaga tttaattttt ttggattata tatattaaca tgaaaattta ttaaaacctc
    20101 ttaatagata tgacttttca agagttatat ctattaagag gtttatttaa tgttcacaga
    20161 taagatatat ttaaataatt taaacaagat tgtcagaaag ttatataatc ttaaaaattt
    20221 atataactct aattcttatt tatttctgaa ataatatcac tcttcaaaat ttgtcttact
    20281 ggaataatta cagcaactat actaatagtg atatttaaaa taatcatacc tatgtataga
    20341 ggtgtattta ttttaagaat tggatttata tataaatata cacgttttac cataaaataa
    20401 atcataaatt gtccaataac acttccaatt accattataa tacttgcata gaatccatat
    20461 aaaaaacctt ctcttatcat cataagtaaa aacttattat ccgtaatacc catagctctt
    20521 aaaattccaa actcatgtct tcttgaaaaa attaagtagc ttactgtatt gattatatgg
    20581 aacaaactta ttgcaagaag tacaaataca ataccatata taaataagag tttttgattt
    20641 aagtaggtat tatttttttc aatagcaatg gtataatctg ttacgataca tctttttaca
    20701 tcacttacaa cactttttat gttctcagac aattcctcac tgttactatc ttctgtcttt
    20761 tttatactta ctgaattata attttcgata tcaaagttat ttttcatttg cccattcgtc
    20821 ataactatat ccataccata atcaccaatg aaatatatgt tatttgccat cacacgtttt
    20881 acagtagcag caactacaaa ttcttttgtt gtataatcat tcttattttc ttctttaaat
    20941 ttaacaattt catctgttgg tttcattgtc tttggcactt taacttttat tgtatctcct
    21001 ggatttatat caactgcatc atacatccca gttccatcca taggtagtct aactataacc
    21061 ttattatttt taaccatatc atgggtatta atctccccat ctataatata agaatttaat
    21121 ccttctaaca taccattgtt ataaccataa atatttgttt ttaaaagata gccaccttca
    21181 ttttgctttg tacaaatacc atcaaaaaaa gtctttatcc tgtaatcttt ttcaagaggt
    21241 ttaaagaaat ttttccaaaa tagttgttct tctttaattt gcatagctcc aaataaatat
    21301 cttactggat agacttttga tattccctca acattgttta atcttgaaat tttatctttt
    21361 ggtataccta catcaaaatc tgttgtttgc atagttattt ggtagtcaga atttaaacca
    21421 tcatcagctt tcattgtaag ttcattatta tttttagtaa gctgtataga gtaactagaa
    21481 catagaaata taattccacc aagtgataat gaaaacaaaa ttccaataaa cattcccttt
    21541 ttttgagtca tgtatttatg agataatact tttgtcatgt tagagtgttt tacactttct
    21601 aatattctct ttttcttatt agtaatctta aagtttttca tagattctat actagagtat
    21661 ttattcaact gatgtacaac acgtacagca attaaaatta acaacaataa gataaataaa
    21721 aagcctaaat aaatagattt ttcacttata aaaaatgctc ctggaacaat atcactactc
    21781 aaaaatatat gtggaaattg ttcatatatt gtagacgcta ctccaatacc taagaagcaa
    21841 cctataggaa atccaacaat aaaaagtaaa aataactcta aaaatagtag aaataaaagt
    21901 tgtttattat tagcgcctaa tacttctaaa accccatatt gtgatattct ctgaagtatt
    21961 gaaacatgga aaatactata tactgctgct gcaccaaaca ctccaacaag caatataatt
    22021 gcatttgtat ttagttctaa attttttaag aactcaaata tattatattt ttctatattt
    22081 tttggtggtt ttactccaaa ttcaactgaa agagaccagt ttatataagt tttttctttt
    22141 gatatgttga attttttcat aaatttataa ttttgctttt taatattttt attttcatca
    22201 tattttacat aaactatata agaatccttt tctttaggct gaaaataatc gtttacaaat
    22261 ccctccagag cagtttctgg gtttgaaaga cttgataaaa taccacataa cttatagatt
    22321 ttaccatcta aattgatttc tgttccaatt tctttgttta catttaaatt gtttagagaa
    22381 tatgtgtcaa gtactatctc tccatcttta ctaggaagag ttccttttaa taattttcta
    22441 cctgtcatgt ctaaatatga agaatcagca tttaaaaatt ctataataaa aggttcatct
    22501 atctgatttt taggttttgt aacgccaagt ctatctacag tgtattcctt tgatttttta
    22561 ttttctttaa ttttattaag ttgttcctta tctactttat agaaataatg ataatctcca
    22621 ctatcttccc ttatcttttc aattctactt ttgtctgcac tatgaactaa agaaccaatt
    22681 cccgtcaata atgctactga tagtactatg cttaaaacta tagatataga ttgtttttta
    22741 taatgcttta tatattgaat tgaaaatttt actagagaca tcatagtaat tcaccaccag
    22801 tattaaccac aactccatct tcaatatgga taattgagtc gcatacttgt gctatatttt
    22861 cattgtgtgt taccataagt atcgtttgat ggaattttgc actactttct tttaataaac
    22921 ataccacttc cattgtagtt ttactatcga gatttcctgt tggttcatct gctaatataa
    22981 gcgcaggttt atttgccaaa gctctagcaa ttgctaccct ctgttgttga ccaccagata
    23041 gttcgcttgg aaattttaaa cgcttatccc aaattcccaa tgtcttcatc agcatttcaa
    23101 tatattcatg gtctatatgg tttcctttat ctaatgtaac aggaagagct atattatcat
    23161 aagtgtttaa tacgggcatt aaactgtagt tttgaaatat aaatcctata tttcttcttc
    23221 taaacactgt caaatctttt tttcctattt tagatatgtt ctttccttta attattacat
    23281 cacctgaggt tggattatct aatccaccta taatatttaa caatgtgctt tttccagaac
    23341 cagaagttcc aactatagca gtaaatgtat ctcgttcaat ttctatactt actccattta
    23401 aggcttttac tttagtctca ccttttccat agtgttttac taaattattc gtcactaata
    23461 tactcattta atttctcctt tcaatacaga tatatgttct ataaaatatt gtaagtgacg
    23521 ataattacac tcatcctaca aaattagttt aaactcttac aattctgtaa gaagatacta
    23581 aaacaacatc cacttcctag tttagattct actataatgt ttccattttc tttttctaaa
    23641 attaatcttg taagatatag accaatccct gtgccatttt ggttttcaac atttttactt
    23701 ctgtaaaatc ttttaaaaat atcatttagt tctctactat caatacctat tccctcatct
    23761 ttaatagtaa tcttcgtata tagttccatt tttattacag aaatagtaat cgtgctatct
    23821 gatggtgagt attttattgc attttctaaa acatttacaa ttgcttctat ggtccacttt
    23881 ttattatgaa ctaattttat gtctgaaaat ggttcgagat ttatccttat atttttagct
    23941 tctgctttat taaagatagt actgatactt tgaataagag tatctttaat tagtagttct
    24001 tctacttcaa attctataac tccatcctct aatttagtca ttttaaataa ggattgtaac
    24061 agccagtcaa tcttaaaagt ttcatttttc atcttttgta aaaactcctt cttctgtaat
    24121 gtggaaatat ttatattctc cagaagttca caataaatgg ttatatttga aagtggagtt
    24181 tttaattgat gtgacatatt tgatattaaa cctttaatcg cttctttttc tgactttgac
    24241 tgttctacct ctatttctat catatcctta atttttttta cttggtgaga aatttttgac
    24301 gctcttgtat cctttgtatc tggataagta acttctttac cttccaatat ttgatttaac
    24361 atcaaattta attcatcata tctttttcga tttattttag tagatactat aaatataaca
    24421 actgtacata ttataaaggc taatataaac atattattct acctcattcc ataaataacc
    24481 cattccataa gctgttttta tatattttgg attagatggg tcatcctcta cttttactct
    24541 aagtcttctt atatttacag atacaatatt atcatcaaca taattttctt cgctgtccca
    24601 aatatggtgt aaaatttgtt cctttaataa tatttgacct ttattttcaa ttagataact
    24661 tagaagctta tattctacag agctaagttc caaacattct ttatttttat atacttttag
    24721 taacttttgg tccaatttta ttccattgga atatattact ttattattcg atttttttct
    24781 tattgctact ttgactcttg cctttaatac agataaagaa aatggttttg taatataatc
    24841 atcaagccct atttctaatc cttttacttc atcaatttct gagtctcttg cagtaagcat
    24901 tataattggt atatctgact tttcccttat tatttgacat acatcaaatc catctccatc
    24961 tggaagattg cagtctaaga ttataaaatc tatattttta ctattaaata tatttaatcc
    25021 ttcttgtttt gtataagcct taaatacatt aaatccatca ttctcaaatg cataaaaaag
    25081 accttcattt aagtttatat catcttcaat aattaataag ttcaatttta ctctccttta
    25141 gattttttag ataaataatt attttcttgc attatcatac taaaaatatt ttaattttat
    25201 ttatttcact aaaaagattg tatttaaatt tgttttcaaa ataaaaacat tataatcaag
    25261 catatagtta tatatttccc aatttaatta cgcttaaatt gaaaatataa ctttattctt
    25321 atataattaa attatacaac aataaagtta tattttttaa atatattttt actaggggga
    25381 atagaattga aaagggttga tgagagaaat actatgtttg caagagcaaa ttacaaaaaa
    25441 gatagtactg cttataatga ctattataag aaaaatcctg acaaaaaaga aattgatgat
    25501 agtataagaa atagaccaaa tttatgtagt gaaggaacaa tgacttacaa tgagttaaac
    25561 tctccaatgg caagtagtgc attcgatttt ttatctgata tcaaatcttt atgcgaagga
    25621 aaagtttctg atactaaagt agatgtagat gcaaagtcaa tgacaaaaaa aattaagggt
    25681 cttgcaaagc agtatggagc ttctgtagtt ggtataacta aattaaaaga ctatcatatt
    25741 tatacaaata ggggtagaca tgaagaaaat tacggtgaag aaattaatct aactcataag
    25801 tatgcaattg tatttggatg tgagatggat aaagaaatga taaatagagc tccaatgata
    25861 tgtgaagtta tagaaacatc aaagtgctat gtggatgcat ctatcgtcgg catgatttta
    25921 agttactata taagaaattt aggatatgat gctagaaatc atatggatgc taattattta
    25981 gtaatgcctg tttttatagc tcgtgatgct ggtttaggag atataggaag aaatgctata
    26041 cttacaaata aagattatgg ctctagactt agattaggtg ttgttactac agatattcct
    26101 cttttagaag atgaatatgt tgattttgga ctagaagatt tttgtaaggt ctgtaaaaaa
    26161 tgctctttta actgcccttc tcattcgctt tctaatgata ttaaaattgg tgatgatgga
    26221 aaatataatt gggtaattga acatgagaca tgctatatta aatggagata tttaggtaca
    26281 gattgtggta tgtgtatatc tgcttgccca tttagccaaa atctcgaaac tataaagaat
    26341 actacttcat ttaagggtaa taatgagctt attcaaaaag ctttagatga gtatacttca
    26401 aaatttggta aaagaatatt tatacctgga aatccatctt ggttgaaata attttattta
    26461 ataaagaatc tgtactagaa tttatcatag gacaggttct ttattaaata tataaatttt
    26521 taaatacttt ttctcgttta taacaattca gcaatgtttt tagtctataa ttgacattat
    26581 aaaatattaa actaaaagga gtaactgtat ggaaaaaata ttagtagtag aagatgattc
    26641 tatattaaat aaaactctat catataacct aattgaagat ggatatataa ttacttcaaa
    26701 atttacagct aaatctgcac taaagtctat ttttgaatgt gaatttgatt taataatttt
    26761 ggatattaat ttaccagata aaagtgggtt tgaattatgt aatgaaataa aggaaaatta
    26821 caatatacca ataatttttt taactgctaa tgatatggaa tgtgatatga ttaaaggcta
    26881 tgaacttgga gctttggatt atataacaaa accatttaat atcaatatat ttaaacaaaa
    26941 aattaaagct tttttaaatc atcttacaat aaaaacaaaa caagattact ataaggatgg
    27001 atatcttgaa attaactttt ctgagttatc tgccaatata aatggaaatc aaattatatt
    27061 tacaccatta gaatatcgta ctttaaaact gcttactgaa aatcctaaaa gtattttaac
    27121 aaggaaagta cttttagaaa aactctggga tatagatgct aactttgtag atgaacatac
    27181 tttaacatct gttataagta ggattagaag caaaattgaa aaagacaatc ttaagtatat
    27241 caaaacggta tatggtatgg gatatatgtg gttaggtgaa agaaatgaat tttaaaaaat
    27301 tttcttttag ttctatttat ttaataattg ctgttggtat aatttttaca gctattttat
    27361 ttagtatctc aatttatcta ataactggtg atttaaaact aattgctcta ttattaatat
    27421 atacaatggt actttgtgga tttatagcat taataatatt tttacttcat aaaaaaatag
    27481 ttatattttg ttctaaaata tgtcacactt tagatagtat gatgaatagt gaagataata
    27541 ttgaaattga attagaagaa gaaactattt tatcacgtat caatcacaga ttaattagaa
    27601 tgtatgaagt tattcaagaa aatcgcaata gtattgctat agagaaagct gatttacaag
    27661 aactagtttc tgatatatct catcaaatta aaactcctat tgcaaattta aaaatgatta
    27721 atactactct actaaatcaa acatttaaaa cagaaattca aaaggatttt ttaatggata
    27781 tggaaaacca attagataaa ttagattttt taatgcaatc tatggtaaaa acctccagac
    27841 tagagacagg tataatcaca ctatctaaaa agaaaaattc aatttatgaa acaattgcta
    27901 cagcacttag tggaattttg tttgatgctg aaaaaaagaa tattgaagtt acagttgatt
    27961 gtgacccaaa tttatatata attcatgaca agaaatggac ttctgaagct atttttaata
    28021 tattagataa tgctgtaaaa tacacaagtt ctaatggtaa gatacgtgta gctacagaat
    28081 gttgggttat gtatacaaaa atagatatta ttgataatgg aaaaggaata tcagaaagtc
    28141 gccaagcaga aatctttaaa agattttatc gtgaagaaga tgtccatgac atagaaggta
    28201 ttggaatagg tctctatctt tcaagaaaaa ttatatctct tcaaggtggc tatattaaag
    28261 ttacatcaga tattggaaaa gggtcgactt tttctatttt cttacctaat aaataaattt
    28321 ttaaaaattt catttgtaat gattctgcaa cattttggtt ttatactgta tttacaagat
    28381 gttaaggagg tattattatg agtattttac aagtaattaa tttaaagaaa tattatggac
    28441 aaagttcaaa cattacaaaa gctcttgatg gtgtaactct atctattgaa aaaggcgaat
    28501 ttatttctat tgtaggtaca tctggtagtg gcaaatccac attattaaat atgatgggag
    28561 gattagataa tcctacatct ggaaaggtta ttataaaggg taaggaatta tctactatgt
    28621 ctgatgaaca gcttacaatc tttagacgca gaaatatagg ttttatattt caaaattata
    28681 atcttgttcc tttacttaat gtatatgaaa atattatctt accaatagga ttggatggag
    28741 agaccataga taaggatttt ataaatgaaa ttataaatat gctttctctt gaagaaaaat
    28801 tatatgatat accaagtaat ctttctggtg gtcaacagca acgtgttgct atagctagag
    28861 cacttgtaac aaaaccagct attatactag ctgatgaacc aacaggtaac cttgatagta
    28921 gaactagttc agatgtactg agtttactta aagtgacaag taaaaaattc aaccaaacaa
    28981 ttgtaatgat tacacacaat aatgaaattg cacaactaac tgatagaatt attcgtattg
    29041 aagatggaaa aattgttaac taggaggtgt agtatatgtt tagaaataat aatcgcacat
    29101 ttataagaaa aatagcttta aatgatttaa aaattaataa acttaaaaca tatctttcag
    29161 gtatcattat aatgatttct acatgtttat tattaacagt gaccttagtt tcatataatg
    29221 cctctgtaga tttagttaat gcatctccat atcatgctat atataaatct gtagatgaaa
    29281 aagcaaaaaa tatactatat aaaaataaaa actttagtgg cgtaggaact tacaaattaa
    29341 ttggaagtaa taaaaagaca gattgtatta tgtcgattgt ttatgcagat gatacagcaa
    29401 ttaaacttat gaattttcaa ccattgaaag gcaaactacc tacaaataaa aatgaaattg
    29461 ctatttcaga aaaatattta caggaatttg gtttagataa agatattgga gatagcatta
    29521 aattaaatta ttatgatgat attacgaata aagaagttca atgtgatttt attattgttg
    29581 gatttctaga aaattattat caagataatg ccaagcaata ctatacagtc gtttcaaatg
    29641 attattttaa gaatatagct tctacacctt tacaaaataa aagtaatact tttgacgagt
    29701 caagacctga tactgtagat gtattagtta aactatcaga agaaaataca gagaaagatt
    29761 cagcttcaat taagacacag ttaaataaaa ttggtttatc tttaggtatt aagaaatata
    29821 atatatactt aaatgataac tatattgaat caaatttaat tgatggtgag cagattatta
    29881 cagttttagt agttggaata gttgttcttt tttctagcgt ttttgttata tatagtattt
    29941 tttatatttc agttgttaat tcagttcaga tgtatgctaa gttgaaatcg ttaggtatga
    30001 cttcttttca gttaaaaaaa attatttctc tacaaggaaa tattttgtca attattttta
    30061 ttccattagg ggttatagct tcttgtataa ttgcttacat aattcaacca ttagcatggc
    30121 aaatgatagc tgatttattt ataattttaa ttttatcact ggtaatgttt ttaactgtta
    30181 gaatttcttt atttaaacca gctagaatta tttctaaaat atctgctatt gaagccatgc
    30241 aatatacaga aactaaattt agaaaaaaag caaaaaattt tagttatata aatataaaaa
    30301 atctagcatt aaagaatatc gagtcaaata gaaagaaaaa tttgattgca ttaatatcgc
    30361 taagcattag tggagtttta tttatttcaa tcgctaatct tgctaattca atagatttca
    30421 aaaaacaact atctcaacaa tttgtctata acgaagatta tataattagc attaagagtg
    30481 ataacttata tgagcatatt acagaaatcc aacaaaataa tccactaaca gattctctta
    30541 agaatgaaat taaatctatt catggagtaa aaaaattaat tgaatccaaa tcaattaaaa
    30601 gtaatattat agaaccttac atcaaagata gagacgaaaa acaatttatt actctaatta
    30661 aaggtattac gcctgaactt tcaaaatcac ttgaagaata tattgtcagt ggaaaaatag
    30721 actatacaca tttagattca gatagtttaa ttataaataa atatagaaca aaatattatg
    30781 gattaaattt aaaagttgga gatacagtta catttaatat ctttagtgga aatgatatta
    30841 ttaaaaagaa aatgaaaatt ataggaatca ttgacaattc caatacagga ggtatgtttt
    30901 ttgcttcaga caaaacttta gaatctctaa caccatataa tacaaattta gatttttcta
    30961 ttttaataga taaaaagcat tctaaagaag ttgaagaaaa gttgttaagt ataattcaga
    31021 aaaattcaaa tttaatgctt tattcatatg aagatgatta tagaatgatt actagagcat
    31081 ttcaatatat aattatagct tcatacattt ttgttggttt aatttcttgc tttggtatac
    31141 taaatatgac caatacccta ataaacagcg ttttaattag aaaaaaagag tttgcattac
    31201 ttcaagccgt tggaatgaca agaaagcaac tgcgaaatat gctttataga gaaggtctaa
    31261 acataagtat caaagctata tgtacttcaa gtacactagg ttattttggt tcaaatctac
    31321 tttgcacttt tataaaagat gtaattagat tagactttat taattttaaa ttttctatat
    31381 ttactatttt aattttttca ttcgtattaa ttggtataca agttttagtg actgaattac
    31441 ttgtaagaaa tatagaaaaa aaatcactta cagaacgctt acgttctcag taagaatgta
    31501 tataaaaaat agccattttt tatacaagtt ttaatatgat tttttaaaag ctatagggat
    31561 agaaaatttt gactttacat aattttctat ccctatactt gtttttattt tatacagata
    31621 cacttactta tatctccgag caacatcacc agagacataa acaagcatat tccttatttt
    31681 ctaaatatgt caattgcatg acttgtaacc cctactaaat cttcacgttc acaagtagca
    31741 aggtgatatt ttaaatataa tgcaaatgca tcatcatcca tagagtctac tgcttcacgc
    31801 atatataaag ccaaaccatc tgatgcgaca taatgcagtc tagttacatt aaatgcagac
    31861 atcaaatcat caatgttttc tttacgtaca agctcaaaca aatcctttgg ttcagactta
    31921 gcagcaaatg tctgtggatc aatcaaacct ttttcaatat attcagatac attaatattt
    31981 cctctatgga acccctcgtc aatgagacag ccatcagata taacatatgc agcaaaaacc
    32041 acaccaccag gttttgttac acgaattgcc tcatgtaaag cttgttgttt atcttctttg
    32101 ttataaagat gatatagtgg tcctaacagt aacgtaatat catatttatt atctgggaaa
    32161 acagataaat ccatagcatt accctgagta atgcttatat tttcatttga ttgagtattt
    32221 ttatggaaaa cctctatatt atgttcgact aattccactg cgtcaacatc atatccctga
    32281 cgtgccagtg tgtgagaata acgaccagtt gcagcgccaa tttcaagcac acaatcacct
    32341 gattttatgt acttatcaat gtaatgcata gtagtgagaa attccaccat tccatgtttt
    32401 aatgcaagtc gactatcttc atcataagta ttataaaagt caattaagta ttgttttgtt
    32461 tccataattt cacctcttct atattagtta ataaacatca aatagacaaa ttccaatctg
    32521 gtttttcttg tcttataaaa gctgttaata tatttttcaa cattttttca gcactatatc
    32581 tatctaatgt taattttttt gttgctgtta agacagctat gccatgagta taaagaaata
    32641 aatctaaaaa tattactttt gcacattcca actctattcc aatgctttcc gaaaaaagtc
    32701 ttgccttttt ttcattgtca atttctttat aaaaatcatt tgcctcttcc atcatcaaat
    32761 ctaaatcatt tataaataac aacttaaata aatgagtttc ttcttgagca aattcaatgt
    32821 atgaaagcgg aagcagtaaa taaggactta cacttttaga attactataa ttaacaacat
    32881 actgattata gtattcatat gcaaaagcaa gaaattcatt ttttaattca ttcatgtttt
    32941 cgtaacaagt aaaaattggt cgagtggaac actgtaattt acttgcaata ctcctcgcat
    33001 ttacagcttc aaatcccact tctcttgtaa tatccaagac tgtattcaaa atcatttctt
    33061 ttgttatctt tactttaggt ggcatatatg ttcacctctc cattaaattt ttataaaaca
    33121 catatgttat ataactatag taacatatca agaaattatg tcaagaaaaa gctttgcatt
    33181 tttttgcaat tcctaaaact tataaataaa acatgagttg caattaatct attaatagat
    33241 aacagcaact cagattttcc tttaaatact cttctttttg tctaatattt catatacaca
    33301 atcttctatt gaattaatat ctttttcttt tcttttttta gaatcaatta aaatgaaata
    33361 cagtataaaa cctacagcaa gtccccatgt tccgccttta gcagcaatta cacctcccat
    33421 aagtccaatc aatcccttct caacacctgt attacatagc tccattgata gctttgtaca
    33481 taaatatcct tgaactaaaa gtgttactga caatgctaat ggtataagag gtgtagcaat
    33541 ctgcgcaatt ggataaaaac agataaaaat tgctgaaacc catactaatg tacccatgcc
    33601 actaaaaaca gactgcatgg cttcctttcc aaccttgtac ctttgagcaa cacttcctgt
    33661 aagtgtcgca gctaatggac cgcacattgg tatatatgga gaaaagattg ccataaaaat
    33721 atttcttatc ccacttataa cattagacct atttgaattg aaatctatat attcatcttt
    33781 tctattttca ctttcagaaa gtactaagtt ttcacctgtt ataaaatcac caaaggcaat
    33841 tatgtatatc ataaaagcca ttggtatccc ttgtataaat aacattacac ttggaaaacc
    33901 tattccaaaa ggagatactg tatttatcag atttttaaaa tctactatgt aaactaaatt
    33961 gtcaaaacta aatctaggaa tttctatttc tttactaaat atacctacta ttaaactcac
    34021 taaaatagct gatactacac catattctcc taaatgaaat aaaaatttat tcttaacttt
    34081 tagagtatca aatctttcag aaaacattac aaaacaagca accaaaattc ctactgttat
    34141 agatatagga taagaatcaa atctcccttt aggattaaac tctgatatta tagtcgatat
    34201 accgacaccc attagtattc cacctttaat agaatttggt atcttactaa ctaactttcc
    34261 agctacacca gttataccca ttaaaataaa tattattcct aaatctattt gtaaagctgt
    34321 taatgcttgt attctttcaa tgcccatgct gaactttaac aaatatgcag ttgtaagagt
    34381 tatagaagct gtaatccagc caggtacaac tgggtctccc ataaaaacat gcaaacagta
    34441 taatattgag ttaatgatag ccatactcca tgccacttca tagggtaatc ccaaaatttg
    34501 tgtactaagg ccaattgtgc ctaaacttgt catacttgat attatacctt gaatagcttc
    34561 tggtttctca aatctgtaat gtataaatgg aattcttatc ttaaacattc caaattttat
    34621 atgaggttgt tcccttctag cgagtctttc cataacttca ctccaccttt caataactct
    34681 tataagttta caactttata attgtttata aattatcaat gtcattaaaa ttagtatata
    34741 acatgcaaat tatataaaca ataaattaaa tctataaaat tgatagattt tgtgcatata
    34801 tagaaagacc gcttcatttt tagttactta aatcacttaa tattagttat ttaaatcact
    34861 tgataaaggt tttttataaa attcaatagt tctattccat gattcaaact gtccttttga
    34921 atttccttct acagttccac ctgaagaaaa aatccctcta ctcatactca tacataaagt
    34981 ctcctttaga ggcataatat atggaattgg taacggataa cccatattat tgtatgttaa
    35041 taattctacg ttatatggat aattattttc ttcaaagatt tctaatgcct ctaaacaagc
    35101 ttcataacta ttccatatgt tatcttcttt tccagcaatc ataagtatat ctgctttgga
    35161 attttctatt tttattctag cctcttttct attctctgct cttttaagac ttttcttata
    35221 agtactatta aatccaaatg gaatatcttt tgatatattt tttttctgtt cttcaaagaa
    35281 tatatcattg tctactttga tatatggaaa tggctctcct ttatatgacc atgatgaagt
    35341 atcatttcca ctcatcaatc cattaagagc ttggaaacaa taaatgtgag gttgtgatac
    35401 tacaaccttt ttaatttgtt catatcttga agcaagtaaa aaggccaatt ctccaccctt
    35461 tgatgtccca tgtacaaata tttctttagt actggtaatt tcattttcac taatccactc
    35521 aaatactttt tcaaaatatt ctaatggaac ctcttctaat ttatctggta acccttctaa
    35581 agcaaagtat gcaactgtta gtacattgaa tcctctagat gcaagtggag ctgctaaaag
    35641 gtctagagcg tctaaatttc catcagaacc acctagcatt aaaattgttt tattattaga
    35701 attttgttta taaaataatt ttcctataaa ctcatcagat atgtattttg tggtaatatc
    35761 ttctgattta aatattctct ttaattcaac ttcttttctt tcaatagaag tttcaaatat
    35821 catattcata attattggtt tatcaataga tatattttct gcaaaatttt taccttcagt
    35881 tttagatttt tccagcgaat aaattaaccc catacaatta tcagccttat aagtaccttc
    35941 tataggttca actaaatcta aatctacttc tcctttttca tttgaacaaa acactccata
    36001 agatgaaaat tcttctccct tacaccatgg aaattccatt ttcacactta ttttaagttt
    36061 agtatttgga attaagtctg aaaccataat cttaagtctt tcatccacaa atgattctac
    36121 attgtataca tttactttca taatctattt ctccttataa taattattta tgttttatat
    36181 aaaacagctt tatctaatac aagataaata aatttttaaa ttaagacacc atattgacta
    36241 tttcatttta tcaacatatt tcatctttgt caacatattg tcttttttag taatttaaaa
    36301 ttatggtcta tatgaaatcc agttgatttt tggctaatta aagataggaa tgctattagg
    36361 tttagaatat atatattcaa aataaaaaac cactcattta aagaatggtt tttaatataa
    36421 actatttgtt attaataagg atattttttt atttaaattt atataatcga acatctaata
    36481 tttaatgttt aataaaatta tgcatttgca aaagtatata tggcattaac tcttgctaca
    36541 tataaattct catcactaca tccaatatat ctagcatatt tttcaatctt ttcttttgca
    36601 ttttcaacat cattttcatc gctctccatt aactcattta tataaatatc cattaatgac
    36661 gctttatatt ctcttatatc atagcctaaa aattcagtca atctctcaat ttccttacta
    36721 aaatctccat cttcttttac caaaaattcg tatacatctt ccctatatgg atttaaagtt
    36781 attatttgtt ttactacttt gcattcgtca gccttcccaa taaaaccatc tttataatta
    36841 ttaaataagg cattagattt ttttatagat tcttcatcaa tataagaatg taccgcagtt
    36901 atacgatttt ctataagggc atctacaaca gcataatgaa ttttaaaaca atcattgtat
    36961 aaataattgc tcattttctt tattgtcgat tctatttcta agaagctcgc gtggccattt
    37021 tttttatttt tatttggtat aataaaagtt ttattcaaat tattaaaaag tctcttaaaa
    37081 tctaaatatt tttcacaata aacactttta aaggtatcga tatctatagt tataattcca
    37141 aaacttacta tagtctcaat tcctttctta aaagactctt ctatatattt ttctcctaaa
    37201 cttaaacctc tatcagatat ttgctttaga ttttgaaaac tatttaaaca atctctttca
    37261 aagtttttag atatttctaa agcatataat ttaaagattt tccttatctt attataatta
    37321 ttttttgtat catcaaatct gataatagct ccatttacat caaaagtaat cattgagttt
    37381 taacctcccc attagtttca tcaataaaat aaatttaata taaactttat aatcataata
    37441 ttaaacttat ttatactata attattaatt tgatgataaa agctattttc cttctaaata
    37501 aaaaatttgt ctaaagtttc taaaaaattt agacaaatct taggttatta ctctttaaat
    37561 tatttttaaa ttattccaaa tacgccaaat aatattggta taaatatagc tggtaacaaa
    37621 ttggctactt ttatattcgt taatttaagt acatttagac caagtcctat aattaataaa
    37681 cttccagctg ctgtcatttc tgtgactaca ggatcactta aaaatccatt taaaaatgat
    37741 gctcccacac atataatacc ttcatacaaa aatacagata ttgcagaaaa cattacacct
    37801 atacctaaag aagcagtaaa tataattgaa gctattccat ctataacaga ttttacaaat
    37861 aaagtatcat gacttcctgt aagaccactt tctaatgaac caactactgc catggctcct
    37921 atacagaata ataaactaga tgatataaaa ccctctgcta tagaatcttt tttatttcct
    37981 ttagaaaatt tactttgtaa aaaagcaccc aacttattta cccatttgtc aatatctatt
    38041 aattctccta tcagagcacc tacagctacg gatattattg tcactaatgt gttttttcct
    38101 tctagtgctc ctgatatacc aatgtacaat acacataaag caattccatt cattatagta
    38161 tcgcttactc tttgaggtaa tcctcctttt attataagac ctactataca tccacctgca
    38221 attgctagac tatttgctat tactccaaac attttttctc ccctcgatta gctaattgaa
    38281 atttatccta ttattaaaac ctaaacatct atactttgta ttggatattt taaatattcc
    38341 aaaaattctt caaaaccttt tctagatata tttacagttt tagtatttat gttaggatga
    38401 aaatttaagt aatcttcttt aattacatct ttatctatgt aaagctcaac ttttctttct
    38461 gtatcattta ttaatgaaaa aggatttaca cttccaggaa gaagtttaag tacattgtat
    38521 agtctttcag gtgaagcaaa cgataatcta gaacttccta tcttatcttt aagtgagttt
    38581 aaatcaactt gtttttctcc tttaactaca ataagataat acttatttcc tttggcattt
    38641 cttaaaaata aatttttaca ttgacacccc tttgttattc catctaaaat gactaattgc
    38701 tctgctgtat atacagcttc atgctctact acatcatatt caacatttaa tttatccaaa
    38761 atatcatata ttttagtttc attcatgcta gattcatttt catcttgttc ttttttatta
    38821 tttaaatcac tcatactttt ctccttaaac acttcatcat aatatcattg tgaggtatta
    38881 ttctactata gctattgctc ttactggagc tccatcagca tcattatact taaatggtgc
    38941 tgctataaat agaaattttt ctggaacatt ttctaaattt gtaagatttt ctatgattat
    39001 ttttcctttt tcaaataaaa tatgatgcac ttcaaatact gatgtgtcat atctatccac
    39061 tgatagcata tctattccta tgccttttat attagtatta gctatatagt ttgcagcttc
    39121 ttttgttaat gtaggataac ctacatagta ctgcttttta tcccaaaatt ttgaccaacc
    39181 tgatttaaat attataaaat cactattttt tatttcatct tcatattttt ttagatattc
    39241 taattctata ttctcatttc ccttttcaag ttccaataca taagcttttc caacaaaatt
    39301 ttctatatcc aacttatcta gggtctcacc tttagtatac atatgtcttg gtgaatccat
    39361 atgagttcca ttgtgagaaa atacactaat taatgtttct tgataaccat tttcttcaat
    39421 aatagctact ttcttgatat ctggtctatt aggctctgca tatacaggca tgtcattatg
    39481 tgtcacatga gttaaatcaa aaaccttcat ctttatatac cccctatgtt tttattttct
    39541 attatatacc taaattcatt ttatttctat cagattttat tccattatat accatactac
    39601 tatatatttt ttataaacct caagggattt accgaaaaat cttggaaaga attatcagta
    39661 aatataacat cttcacccaa aaaacgttta taccttcctt cttctttctt taaagtaatt
    39721 ctctgttttt gtgaatgatt attaaaaacc attatcttaa gaggaggttt ttttctatta
    39781 gtctgcactc tttttaacat gtattttatg tgcatatcag cttcaggaaa tgaatatcca
    39841 caaaatatta aaaggtcagt atctcttaaa gttttttctg tttcattcca aacattactt
    39901 aaaaatatgt ttgacatatt cttaaaatat gttggtggca ctataatagg ttctgttaac
    39961 tcaccacact ctaaacattt tgtctctgaa ctattttcaa taagcttcat gacaccacct
    40021 tcatgtggtg taagagttac actattacaa atggggcaat aaagccaatt caatgaccca
    40081 tgtatcttat ataatttgat taatggttct tttggttttc tccaatttaa ctcctcttta
    40141 aaatttgtaa actccacacc ataatcaagc ataattggca aatcattctt atctcttaat
    40201 ccagtaactg tattgtctat atgtatatca taatttgcac taataaaagt agtatcaaga
    40261 agcaaatcat tttccaaaag attttcaact aaaagtttat gatatttatt atctgttcta
    40321 ggtgtattat gaattgcttc agccattagc agtattaaat attgtcttaa aaaacgtata
    40381 ctatcacttc tgttatttag attttctagt ccaaaatttc taaatgcttc ttttctttgt
    40441 tctgctaagt ctaaaagacc taaaacttct tcaaaggtag gaaaatttgc attatctata
    40501 ttatctttta ctatatcaat attgaacatc tgtttaaaga aaatatacaa ttctctattc
    40561 attttggtat tataatttgt gggatttagt tttttaaaat attcgctaaa aagttcattt
    40621 tgaataggta gattctctgc tgctgaagct cctgctccta aaaatattgc tattttcata
    40681 tagttcctcc taatataaat attactccta tttttatatc cattaaatat ccatatcatt
    40741 tattttatga tacaacaata aagctgtctt ttactagaaa aaatcctatt acaagacagc
    40801 cttatattat attaaatctt taataattcg taaatagaaa gagcatatat ttgagtagct
    40861 tttataatat cctctactat taagtattca ttagcttggt gttctgtttc tggttttcct
    40921 ggaaaaatag ctccaaatgc aacacaatta tctaaagctc ttgcgtatgt tgctcctcca
    40981 gatgaaagtg gtgttccatc aagaccagtt tcttcttcat atacttttct aagtgtctta
    41041 acaagaagag tatcttctgg aacatagatt gaatccaaga aatcatattc ttcataattt
    41101 aaattgtatt tatctgtcat tttctttaac tcttttacaa aatcatcctt tttgtatgtt
    41161 actggaatcc ttacatctat tcctgcaaat tctttttcgt tatcaatagt tactctacct
    41221 atatttactg ttagtttacc tgatacatca tccttgcaat taggaattat attgtttcca
    41281 ttagcatctt caccaataac ttctgctaaa aacttaatta tattagaatc tatacctata
    41341 ttattaagag ctatacaaag tctagccact gcatttatac cagtatcact tgctgctgaa
    41401 tggacacttt ttccaattat acagatttta ttaccttcaa cagtatactc aaaatttaat
    41461 ttatcaagtt ctttttttaa tttatcagag tattttccaa tatagattgc tttacctgga
    41521 actgcattta aagcctttcc aactgacaac tctatatcac ttttgctatc acaagtcaaa
    41581 tgaacttgta aaagaccttt ctctgcattt gttattggga atctagaatc tggagtaaat
    41641 ccatagtttg gtatttcttc attattttct ttatatttat ttatacatct ccaaagattt
    41701 tcttcatcag ccccaaaaat gaatcttatc ctcttattaa agtctacatt taaatccata
    41761 agagctttca ctgcataaat tgctgatatt gtaggtcctt tatcatcttg tgttcctctt
    41821 ccatacaatt taccatcttc taaaacagct tcaaatggag ggtaattcca gctttctaaa
    41881 tcaccttctg gaaccacatc aacgtgacca agtataccta tcattttttc accttgtcct
    41941 atttctgcat atccataata tccatctttg tatactgtct taaatcctaa agatttacat
    42001 aagtctaata tacctcttaa agctttatct acattttcac caaatgggca attttcagtt
    42061 gcttcagata taacactagg tatttttaca ctttcttgta ttgaagaaat catttcatct
    42121 tgtagagaat ttactttttc tttaatttgc tctttcatat tatcaccctt caattttcat
    42181 cataatatac taataaatat tttgaccttt taaaaaaaaa atatgcttat tttttcagtt
    42241 tttttataaa ttcaatcgct tttaaagtcc ccatttttga atggtcaaat gtaagtccac
    42301 cttgaaaata aacattataa ggaggtctta ttggagcatc tgcacttaat tctatagaag
    42361 aaccttgtat aaatgctcct gctgccatta tgacttcact ttcgtatcca ggcatgtccc
    42421 aaggaacagg ttttacataa gaatcgactg gtgcagcttc ttgtatacct tcacaaaaac
    42481 ttattacttc atcagcatta ttaagtctaa tacattgaat aatatcactt cttaagtcat
    42541 catattttgg taatacatca tatcctaatt tttcaaaagc tcttgagcaa aagatagctc
    42601 ccattacagc ctgagatact atataaggcg ccaagaaaaa accctgaagt acatttctag
    42661 ttgttccaaa agtaagacca cattcttttc ctattccagg agaagtcatt ctataagata
    42721 taagctcaat caaatctttt ctaccagcta tatatccacc tgtcaatgca agcccaccac
    42781 caggattttt tattaaagag ccagccataa catctgctcc aacatcagta ggctctttgg
    42841 tatctaaaaa ctcaccataa caattatcaa ccattactat agcttctggt ttaacagact
    42901 ttattacttc tatagcttct tttatatcac taattgaaag tgattttctc caagaatatc
    42961 cttttgacct ttgtatcata acgagtttag ttctatcatt tattttgttt ttaattcctt
    43021 ctaaatctaa gtttccatcc tctaaaaaat ctacatcatc atatgtaaca ccatactctt
    43081 tcaatgaacc tttttcttct cttatgccta taactccctc taaagtatca tatggtctac
    43141 cagtaacaga taaaatttca tctcctggtc taactatacc ttgtatacat aaagtaagtg
    43201 catgtgtacc atttacaatt attggtctta caagagcatc ttctgtatta aatactttag
    43261 aataaatttc ctctattttt tcacgaccaa tatcattata tccatatcct gtagtccagt
    43321 taaagtgcat atcacttagt ttagattctt gcatagcttt taaaacttta tattgattat
    43381 attctcttat ttcttttatt tcttcaaact tatcttttat ttcttccatt atttcttgcg
    43441 atagtttaaa agtatcatca tctattccat agtagtcttt taataattct tttgtctcat
    43501 taagcatttt atcacctgtc tctttattat tttatttaaa catgtaaagg agactatttt
    43561 actaaaacaa ataaattata gtttataagt tagtataagt ctcctctcaa tcatcttaaa
    43621 ttaaattaat tatctatctg ttgttattat tattgttgtt ttggttttga tttgttagat
    43681 taatatactt tccaggttgt atagttgata cagcatgttt ataaatcatg ttttgttgtc
    43741 tattatcccc ttctattaat attatataac tatcaaaccc ctttacaagc cctttaactt
    43801 gcactccatt aactaaatat atagtaacag gtatcctttc tttccttgca ttatttaaaa
    43861 acaaatcttg taaatttaaa actgtatttt tcattagatt tgtaccctcc caagattaaa
    43921 ttgaacttct aaaatattcc taccttatat tctatataag ttatgacaaa ttttcaatat
    43981 taagttgtat acttaaaaat ttatatatta ttttatagaa tctttaatat ataagattat
    44041 ttcatttttt aattcgtcta tattttcata ttggtctaaa tcaaaccatt ttgctgtttt
    44101 atatctttta aaccatgtta tctgacgttt tgcatacctt ctagaatctc ttttaattat
    44161 ttcaatagct tcttcgtatg tatattctcc atccagatac tttaatatct ctttataacc
    44221 tataccttgc attgatatca tatctttttt aaatcccata cttaaaagtt tttttgcctc
    44281 ttcaaccaat ccatttttaa tcataatatc aactctcata tttattcttt gatacaaatg
    44341 ttctctatct ctatttagaa ctattattat tggttgatat tcttcattaa attttaaatc
    44401 acttgaaaaa tcattcattt tcttaccact tagacaaact tcaagagctc ttatgaccct
    44461 tttagtattg tttttatgga ttctattagc agattcttca tcaagttctt ttaacttatt
    44521 atgcatgtaa tctattccct tttcagcaag ctgtttttga agttcttctc ttaattcatt
    44581 attggcatca gattgtgcaa aatccatatt atatattaaa gagtttaaat aaagtcctgt
    44641 gcctccagtt actaaaacac atttttcctt ttcatttatt tcatgtatat atttttttgc
    44701 tctttgttga aactctgaaa ctgaaaatgg atagtcaggc ttaacttcat ctataaggta
    44761 atgcttcact ccttgcatct cttcctctgt aacttttgca cttccaatat ccatatattc
    44821 atatatttgc atagaatcag ctgaaattat ctctgcattc atatcttttg ctaattttat
    44881 tgacaaatct gtttttccaa cagcagttgg acctgtaagt attataagtg gtattttttt
    44941 cataacattc ctttccatat attttaaata tccaaatatt ttacataatt cttttaaaca
    45001 ttttttctat ttctgtctta gatatttcga ccattatagg tcttccatga ggacatgtaa
    45061 atggattctc acacttttct agctgttcca ataaactttt tatttcaata tcgtatattt
    45121 tatcatttgc ttttatagca gacctacatg ccatagatgc aaatctctct ccttttaaat
    45181 cataattact tgtaatttct tctatattat caattatttg aagtataaat ttctcagttt
    45241 caggtactcc aaatatagta ggaacacatc ttaccattat atgattattg ccaaaaattt
    45301 ctagctcaaa accaaatttc ataaataact ccaagttatt ttcaatttga agcatatcta
    45361 tatttgatac ttcaataacc actgggtcta aaagtatttg catatttata tcttgtctat
    45421 agaacttctc catatatctt tcatatagta ttctttcatg tgcagcatgt tggtccaaca
    45481 aatacataga atcgtctttg ctcaaaatta tataagtatt aaatacaact ccaattacaa
    45541 aatatccgta taaagaaaac ttgcgttttg aactacacaa gtattcttct tcactgtcct
    45601 taatactatc tcctaagtaa taatttcctt catttttaat tccatcaact tgaaactctt
    45661 cttgtatatt atcatttgca ctatcttcac ttagataatt aatgtttttt acatcatctt
    45721 ctacacttgc atttaggact tcacttactg attgaaattc atttattggt ttttcacttg
    45781 ataaagtaac aacttcaatg acttcatcct tacctttagt aatattacta tttttaggag
    45841 tagattctaa taaatcatta tttcttaatt tataatctgt acttttttct ctactgttaa
    45901 tagcgattct aggttgtgtt tttttatcac tataagtagc atattttcca ataagatttg
    45961 agtgtattaa tttaaccttt aaaaaatctc taagttctat atacacttct tgttcttttt
    46021 caaatttaat ttcaagttta ttggggtgta tatttacatc tatacatgat gggtctactt
    46081 ctatatttaa gaagcacact gcatgtttac ctattggaat tatagactta taactttctg
    46141 ttattgcatc tatgattatt ttacttttta caaatctttt atttatatag atatgttgta
    46201 agtttttatt tgaacggtat atattgttat tacctatata tccattcatt ttaaagtgat
    46261 tgcacttaaa ctctacatct attatatttt ccgttatttc ttttccatat atacttctaa
    46321 tagtgtttac aagttttcca tcccctggag tatttagcat ctgtttattg ttatttgtat
    46381 atttaaattg aatgtttgga ttacctattg caagtttatt tatcaaatca cttatgttta
    46441 tagtttctgc atgagtagat tttaaaaatt tctgtcttgc tggtgtatta aagaaaatat
    46501 cttttataat gatggtagtt ccattagtag aacctattgg ttctttcgat attatttttc
    46561 caccttcaac atatatttta gttcctatta tttcatcttt agttttagtc gtcatttcta
    46621 attttgatac agctgaaata gatgccaatg cttctcctct aaaaccaaga gagtataaat
    46681 catacaaatc atcaattttt ttaattttac tagtagcatg tctcaaaaat gatttttcaa
    46741 cttcacttga aggtattcct attccattat ctgtaatctt aattagactt tttcctccat
    46801 caattatatc tatggaaatt ttatttgcac ctgcatcaat ggagttttca ataagttctt
    46861 ttactacaga agaaggtctt tctacaactt cacctgctgc tatcttattt atagtcaaat
    46921 catctaggat gttaattata tttttcattt ttacacctct aagattttat ttcttttgct
    46981 tttctttgaa gattataaag agagtttata gcatctaatg gagtcatatt taaaatatcc
    47041 agatttaaaa tttcttctga tagtatatct ctatttacac tatcaaaaga gatttgagtt
    47101 aaggctactt cttttacaga atcttcttga ttaattttct ctttatcatc attttgcttc
    47161 attaaagcaa cttcatcatt taatgcttta aatttagaat ttaatttatc ataatccttt
    47221 tttatatgtt tgtagtcttt tactataagt tcatgttcta tttttagaga ttcatattta
    47281 ttcttaaaat tactttgatt taccttaact aattcttcgt ccaaattact attaattata
    47341 ttattgtttt ctttatctcc atttattgca acactgttgt aaacatgatt tttttccaag
    47401 tctttaagta tatattttgc tctttctatg acttcgtctg gtaatttagc taatttagca
    47461 acatatattc cataactctt gtctgctccc tgaggaatta tttttcttaa aaatattatt
    47521 ccttccccat cttctttgac tgctattgaa taatttttaa cctctttaaa ctcttcttct
    47581 aaatcagtaa gttcatgata atgtgttgca aataatgtct tacatctaat atttttttga
    47641 atatattcca ctattgacca tgctaaactt ataccatcat aggtactagt tccacgacct
    47701 atttcatcta atataactaa acttctctct gttgcatttt ttaatataag ggatacttcg
    47761 ttcatctcta ccataaaagt agattgacct tgagataaat cgtcactagc tccaactctt
    47821 gtaaatattc tatctaatat tggaatatca gcagactctg ctggtacaaa tgagcctata
    47881 tgtgccataa gagctattat agcagtttgc ctcatatatg ttgatttacc tgacatatta
    47941 ggaccagtta ttatgttaat aatgttttct ccacgattta aataagtatc atttggaacg
    48001 aaattttcct ctcctactat attttcgaca acaggatgtc taccatttct tatatcaagt
    48061 ttattatttt catttattgc tggttttacg tagttattta tatgtgcaac tgttgcaagg
    48121 gacacaaaaa catctatatt agctatagtc ttagccactt tttgtattct atctatgttt
    48181 ttatatattg tatctcttat ctctacaaat atctcgtatt ctaaagattt aatcttttcc
    48241 tctgcatgta gtatcttttc ttctatttcc tttaattctg gagttatata tctttctgca
    48301 ttactaagtg tttgctttct tatatatgtc tcatctagct ttgcctgttt aaagttggct
    48361 tttgttattt ctatataata cccaaaaact ttattaaatc ctatttttaa tgatttaact
    48421 cctgtttttt ctctttccct gttttctatt tcctttacaa gaaaggctcc attcttagaa
    48481 atctctctta gttccttaag ttcatcacta aaatctgatt taattatgtt tccatctttt
    48541 atggttatcg taggttcttc tagaatagct tcatctatta aattgtatat atcatctaat
    48601 ttatccattt ctgaaacata ctcttttagg atttttgcat ttgataaatt tatagtatct
    48661 tttaaattag gtaacttttc tatcgaattt tttaagtgaa ttaactcttt tggtgttact
    48721 ctctcaaatg ctatctttcc acatattctt tctatatcgt atatattttt aagtatatca
    48781 tttaggtctt ctcttaatat aaagtcatct tttatttctt ctattacatc taatctattt
    48841 tcaattttag atttatttat caaaggttct tctacatatt ttcttaaaag tctacctccc
    48901 attgctgtag aagttttgtc aagtacatgt aataatgaac cttttttctt attccctctt
    48961 atagtttgag taagttctaa attagttcta gtaaacatat ctagaaccat atattctgat
    49021 gaattgtata tatttatatt attaatgtta gatgttattt gtttttgagt attgtatata
    49081 taattcagaa gaattgataa gcttgacttt attagacctt tatcatcaaa ctttagtttt
    49141 tgtagataaa catctggaaa atattctttt aatatattta tatccaaata gttatcacta
    49201 aaactttcat ttatgtatat attacttaca gtagctatat ctctaagctt ttctataaag
    49261 tctaaatcat ttataattat ttcagttgga tggatttttg caatttcttc aattacctta
    49321 tcttcattta aacatgttgc attagtttct cctgtactta tatcaacata agttagacct
    49381 atattagtac catccttata caaagataat aaataattat tctttttatt ttcaagtaaa
    49441 tttccatcta aaactgtacc aggagtgatg acccttataa cctctcttct aacaattcct
    49501 tttgctgtag atgggtcttc catttgttca cctattgcta ctttatatcc attttcaact
    49561 aattttgata tatatgaatt ggctgagtgg aaaggaacac cacacatagg agcacgttct
    49621 tctaaaccac aagcttttcc tgtcaatgct atttcaagag cttttgaagc tactaatgca
    49681 tcttcaaaaa acatttcata aaaatctcca agtctaaaaa acagtataca gtctttatat
    49741 ctatttttta cttcaagata ctgtttcatc ataggagtta atttattcat atctatttgc
    49801 attatcaaac ctccttcttt tctaattaaa tattatatca tagtagatga ttattattaa
    49861 cacaatatat aaaattttga catataacta aaaacaatcc aaaatgtata ctcacatttt
    49921 ggattgctaa gtttaatgct tattaaataa aaatattttt ttttacaata aagatattat
    49981 ttctttttta agtttaatga tattttcaag gttatcttca tttaactttc tactatctat
    50041 aacaaattct ttctttacta ttgatggttt ttttgaaata acataaattc tgtttgatag
    50101 cattatagct tcgtcaatat catgagttat aagaagtatt gttgaattta cttttttctt
    50161 aacatctaaa agccattttt gcatggatgt tttagttaat gaatcaagtg ccccaaaagg
    50221 ctcatctaaa agcattatgt cattagaatt tataaaagtt ctcaagaagt tagctctctg
    50281 tctcattcct ccagaaagtt ctgaaggata tttatcttca tatccgctta atccaaacac
    50341 ctcaaaataa ggttttacca ttgacctagc tttactcttt ttttcttttt ttaaaattag
    50401 tggtagtgac acattatcta ttatagtttt atatggcaag agtaaatctt tttgatacat
    50461 ataactatac ttaccattta tattcacttt gccactatca taatctgtaa gtttagttat
    50521 aatattaaaa atagtacttt ttccacatcc acttggccct aatatagaaa caaactcacc
    50581 ttcataaaca tcaatactta tatcttttaa aacttcaata tctttataag tttttgatac
    50641 attttctata gatactttta cttttttatt gtggtaaaaa ttcatttgtg taagcatcac
    50701 ttgccttcat atccttatct attaatttat attcttttaa gaatgatgta tagttgttcc
    50761 atacactatc tttcatttct ccccatcgtg gtgcatcatc tttgtacttg cttgctaaat
    50821 attcttgact ctttaaagct agtttttcat ctacttcagg tgcatgtttt actaaaattt
    50881 ttgctgattc ttctggattt tttattgcat attcataacc ttttgttgta gcttttaaga
    50941 attttttagc aagctctgga ttatttttaa gcacagtttc attagaaatt ataagtggtg
    51001 tataataatc caatctctca tctaaatctt ttactggtat aaagtttaag tcatatccaa
    51061 ttaaatcagc tttaactgca tcccaacctt caaatatcca tgcaaaatct acagttttca
    51121 tagctgcaaa gaaatcatct tgaccagtat taactatctt taacttatta aaatcagctc
    51181 catttttctt cataactgct ttaaaaacag cttcttctga tggagagccc caaccaccat
    51241 aagtttttcc ttcaaaatct ttagctgttg ttatattctt ttcttttggt gatgcaaacc
    51301 cagaagtatt atgttgtata acagttgcta cagcttttat tggaagtgga tcttcactag
    51361 tctttgcata agtaacttgt tcttgatagc ttataccaaa gtcagctttt cctgttgcta
    51421 ctaaagtttc tgctccactt tcaggtggtt gtactatttc aacatccaaa ccttcttctt
    51481 tatagtaacc cttatcaagt gctacaaata atccagtatg atttgtattt ggtgtccaat
    51541 caagcaccat agttactttt ttagaagaag tatctttatc attgtttttg tctttattgt
    51601 tatttgaaga acatccagtt attgttgcca atgtaaaaac acttactaaa cctaatattc
    51661 ctaacttttt aaaattcata attgcctccc tcaaatacct ttaatctatt aatctatgtc
    51721 ttataacaac tttttttaca gcctgtacaa ttcctacaaa cattaaacta aatataacta
    51781 ctaatatagt agatgcaaat actttatcaa gtgcatatgc actttttgac ctaagcatat
    51841 aaacaccaag tcctttcgtt ccacctagcc attctgcaac agttgctgcc attacagcat
    51901 acgttgcaga tattttaagt ccagaaaaaa atttatccat tgccatagga aatttaaggt
    51961 gaatgaatgt attaacttta tttgagtcca tagtcttaaa taaatttaaa taatctttat
    52021 caatattctc aatgccatct acaaaactta ttaatatagg aaaaaaacaa gtaagcatta
    52081 ccatcaatac ttttggcatt gttccaaatc caaaccatat cataaagagt ggtgctataa
    52141 ttattgtagg tatcatctgt gtaaccaaca taataggata aagacatttt tttattattg
    52201 atacaaaatc catcaatatt ccaattacca atgatattat aattgcgact ataaaaccaa
    52261 gtattgcttc tcctatagta accacagcat gtatggataa attttgataa tctttaaaaa
    52321 acacattaat tatatctact ggtgtgggta gtatatactg aggaacttct aaaactccta
    52381 ccatagattg ccaaagaata agtataaaca aaaatgttgc tattggatat atactatctt
    52441 tgactttatc tatgatttct aagtttttca tctatagtca gacctccagc tttttcatct
    52501 actctagcta ctgttatgac tctttcacat ccaagattaa aagggatttt atgtaccttt
    52561 tttaataatt caaaaacttc atcaaaatca ccttcaacag tagtgctcat tgctcctatt
    52621 tcacatttaa gaccactttt ttcaatttca gcaatacaag catctactac tttatacatc
    52681 tcttcttcac ctttgtaagg tcttagtggc attactgcta tgtctgcgat taccatttaa
    52741 atcactcctt taaaaataat taatacctac taggtatatt ttgtttttta ttaaaaaatt
    52801 ttattactaa aaagatatta tatttaaata aaagaaaagt cttatccaaa ataggataag
    52861 acttattttt atcttaactg gtctccctac actggcatta tccaaacagg ttcaaagggt
    52921 cgagataaaa tctcctctca gcacattagc tccccgaaat atattatatc ttttctactt
    52981 gattatacta cacttatcat attttaacaa attttttatt tatattagat attttatccg
    53041 ttcaaaaata tctaatataa attgtacatt attacaccaa ataaattatc ttaaaatggt
    53101 aatttatcaa catcttccaa aactagatta actgaatttt ctagttttta ttttgctatt
    53161 tcaagcatat ttggatgtat atcaattcca tacaaatatt gtgctaaatt tctgttttca
    53221 ctcatctaat ttgcaatcgt tacaaattac agattagaaa tagcttttag actctactta
    53281 tctttatcta caaaaaatcc ttcaataaat tcattaataa tattcagtaa tctaaaaata
    53341 aacctctatc tagattttta agatacaagc cttttaggaa gtatcttttg tgataatata
    53401 aaaattactc atcattatca tcaaaatcct cagcaaccaa cattttagat aagaatcaaa
    53461 ctctataata taattatttt atccaattta tttttgagaa ataaaatatt tttttaattt
    53521 ttaaatt
//
LOCUS       DAEPIN010000020        52917 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.020, whole genome shotgun sequence.
ACCESSION   DAEPIN010000020 DAEPIN010000000
VERSION     DAEPIN010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 52917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52917
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.020"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            complement(<1..896)
                     /locus_tag="KR250_001406"
     CDS             complement(<1..896)
                     /locus_tag="KR250_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HBH0067353.1"
                     /translation="MKLKHITIIGSLFPILFSLVLFFGVLISADSDDENSNFSSGITG
                     MNLSAEVLKHQTMVEKYARENGISEYVNVLLAIIQVESGGTAEDVMQSSESLGLPPSS
                     LDTESSIKQGCKYFASLLSSCKNQDIDDLNVAIQSYNYGGGYVGYVAGNGKKHTFNLA
                     ESFAREKSGGKKVTYTNPIAVAKNGGWRYGYGNMFYVELVNQYLTVPQVSGELAQKVM
                     NEALKYQGWKYVFGGSSPTTSFDCSGLTQWCYGKAGISLPRTAQAQYDATQHLPLSQA
                     KAGDLVFFHSTYNAGSYVTHVG"
     gene            complement(893..3097)
                     /locus_tag="KR250_001407"
     CDS             complement(893..3097)
                     /locus_tag="KR250_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBH0067354.1"
                     /translation="MKERIQSAFTKRKILHFLKMALFVVALSLILLSLLGTVAHATGL
                     VDDTINAENLYSKYPLSNYQLDFFVDNSWSWLPWNWLDGIGKSVQYGLYCITNFVWTI
                     SLYLSNATGYVVQEAYKLDFINDMADSIGKSIQTLAGVTENGFSSTGFYVGFLLLIIL
                     VVGMYVAYTGLIKRETSKALHAVINFVVVFVLSASFIAYAPDYIKKINEFSSDISTAS
                     LDLGTKIMLPNSDSEGKDSVDLIRDSLFSIQVQQPWLLLQFGNSNAEEIGTDRVEALV
                     SASPEDEDGKTREEVVKTEIEDNDNNNLTIPQVVNRLGMVFFLLFFNLGITIFVFLLT
                     GMMLFSQILFIIFAMFLPISFLLSMIPSYESMAKQAIVRVFNTIMTRAGITLIVTVAF
                     SISSMFYNISTDYPFFMVAFLQIVCFAGIYMKLGDLMSMFSLNAGDSQSMGRRIFRRP
                     YLFMRHRARRMEHRIARAVSAGGISGGVAGAVAGSAVAGKRAERKNTASKENRGNTTS
                     SMGQRAGSKVGAVLDTKNKVKDKANAVKENIKDMPTQTAYAVYSAKEKAKSSVSDFKR
                     GMVQEQQSRQTGRLEKQEQHKKSIAVKRMELQKAQEARQAQRKADGSATTGATRPHER
                     PATASTVSKTGAEKMQEVKRPATTPTTKASEPVKTSGVKERPLSSSTPDGKATQSTYR
                     QNVEKVTVSKETRQNYTKDRRTKVQQTQTVQKNQQTTEKIRNLVTKKGQKKK"
     gene            complement(3097..5547)
                     /locus_tag="KR250_001408"
     CDS             complement(3097..5547)
                     /locus_tag="KR250_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBH0067355.1"
                     /translation="MFPIKYIDNNLVWNKDNEVFAYYEMIPYNYSFLSAEQKFIVHDS
                     FRQLIAQSREGKIHALQIATESSIRSMQEQSKKLVTGKLREVAVQKIDEQTEALVSMI
                     GDNQVDYRFFLGFKLMVTEEQLNLKNIKKSAWLTFKEFLHEVNHTLMNDFVSMPNDEI
                     NRYMKMEKLLENKISRRFKVRRLEIHDFGYLMEHLYGRDGIAYEDYEYQLPKKKLKKE
                     TLIKYYDLIRPTRCVIEESQRYLRLEHEDKESYVSYFTVNVIVGELDFPSSEIFYFQQ
                     QQFTFPVDTSMNVEIVENRKALTTVRNKKKELKDLDNHAYQAGSETSSNVVDALDSVD
                     ELETDLDQSKESMYKLSYVIRVSAPDLDELKRRCDEVKDFYDDLNVKLVRPAGDMLGL
                     HSEFLPASKRYINDYVQYVKSDFLAGLGFGATQQLGENTGIYMGYSVDTGRNVYLQPS
                     LASQGVKGTVTNALASAFVGSLGGGKSFCNNLLVYYAVLFGGQALLLDPKSERGNWKE
                     TLPEIAHEINIVNLTSDKDNAGLLDPFVIMKNVKDAESLAIDILTFLTGISSRDGEKF
                     PVLRKAVRSVTQSENRGLLHVIEELRKEDTAISRNIADHIDSFTDYDFAHLLFSDGTV
                     ENAISLDNQLNIIQVADLVLPDKDTTFEEYTTIELLSVSMLIVISTFALDFIHSDRSI
                     FKIVDLDEAWAFLNVAQGETLSNKLVRAGRAMQAGVYFVTQSAYDVSKESLKNNIGLK
                     FAFRSTDINEIKQTLEFFGIDKDDENNQKRLRDLENGQCLLQDLYGRVGVVQIHPVFE
                     ELLHAFDTRPPVQRNEVE"
     gene            complement(5525..5923)
                     /locus_tag="KR250_001409"
     CDS             complement(5525..5923)
                     /locus_tag="KR250_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009274602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBH0067356.1"
                     /translation="MKKIKSYTGIWNVEKVLYAINDFNLPFPVTFTQITWFVITEFII
                     ILFGDMPPLSMIEGAFLKYFGIPVALTWFMSQKTFDGKKPYSFLKSQITYALRPKITY
                     AGKAVKLHKQILNETITAVRSVNYVPDKIY"
     gene            complement(6041..6544)
                     /locus_tag="KR250_001410"
     CDS             complement(6041..6544)
                     /locus_tag="KR250_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBH0067357.1"
                     /translation="MIDDMAVYIANLGRYNEGYLVGAWFTFPIDEEDVKEKIGLNEQY
                     EEYAVHDTDNFPIDIGEYVSIEELNEMYEMIEELPDYIVECLDEFISHYGTLEEVVEH
                     KDDIYFYPDCETMTDVAYYYIDELNALGDIPANLQNYIDYEAYGRDLDMGGRFIETSR
                     GICEIPY"
     gene            complement(6562..7065)
                     /locus_tag="KR250_001411"
     CDS             complement(6562..7065)
                     /locus_tag="KR250_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBH0067358.1"
                     /translation="MQETRVLVETGRTNGEETTSYWFDLPIDVAEFEEKLGVGAESQD
                     YRIIEKVLPYADEVHEHTSVYQLNELDFMYRQLSADMQEEYVELLTVFENLEALYICR
                     NVITVYHDCKSMIDVARQKLMNDPTFKHLSEDCQAYYFDFEAYASHLLENKRYLVTEN
                     SIFELPE"
     gene            complement(6984..7280)
                     /locus_tag="KR250_001412"
     CDS             complement(6984..7280)
                     /locus_tag="KR250_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005339688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBH0067359.1"
                     /translation="MNDIFKDMQAKVGCEYISDLPSYKRKVWHEMKRLNPADYEEKQL
                     KDFSKYVFGMSYQTLKDVMKQQKGREEQCRKQGYWWKREEQMAKKQHHTGLTYR"
     gene            complement(7379..8152)
                     /locus_tag="KR250_001413"
     CDS             complement(7379..8152)
                     /locus_tag="KR250_001413"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0067360.1"
                     /translation="MKGIKVLMISDEMRVDILHAVFINKNFIKDEENCNYEKYLLELV
                     NKSIYFREKSNFAEYVPPKSENHGECDCNSPNYQMDFKLLESTTRLHASKELTGQIQK
                     FCDGVIGKCPPRRPNTQMTVTRLFASLRDYDCESLHSCLTEKYEYGTIEFDIQTYVKL
                     LTFKKNLFFFFPYKFSFNTCYNFKYALDSIRIALEKDFRESNLFREKYYAEYDTFLAY
                     IYEDNLIISKFEQDGKLQMIDCIYLFKSQTYSKLYEYTW"
     gene            complement(8212..8433)
                     /locus_tag="KR250_001414"
     CDS             complement(8212..8433)
                     /locus_tag="KR250_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0067361.1"
                     /translation="MNFGQNLYQWFLSNAQSLVLMAIVVIGIYLGFKREFSKLIGFLV
                     VALIAVGLVFNAGGVKDVLLELFNKIIGA"
     gene            complement(8434..8568)
                     /locus_tag="KR250_001415"
     CDS             complement(8434..8568)
                     /locus_tag="KR250_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017143882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HBH0067362.1"
                     /translation="MWELVKDFLLVSMGMGIGVVLMCILSISKEADYEMKQLEESEDN
                     "
     gene            complement(8581..9777)
                     /locus_tag="KR250_001416"
     CDS             complement(8581..9777)
                     /locus_tag="KR250_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HBH0067363.1"
                     /translation="MVLNEEQWIKELREKRIAYGISQGRLAVASGITREYLNKIESGK
                     MKPSKELLETLHKELARFNPEAPLTMLFDYVKIRFPTLDIQHIIKDILKLNINYMLHE
                     DYGHYSYTEHYSLGDIFIYTSADEEKGVLLELKGRGCRQFESYLLAQQRSWYDFLMDA
                     LIDGGIMKRIDLAINDHTGILDIPELAEKCRKREYIGKSRSYKYYQSGELIKHREDDR
                     EYMGSTLYLGSLKSDVYFCIYEKDYEQYVKLGTPLEEADIINRFEIRLRNERAYYAVR
                     DLLTYYDAEQTAFSIINQYVRFIDEEPDKRKNDWKLNDRWAWFIGDNRQSLKLTTKPE
                     PYTLDRTLRWVQRQVAPTLKMLKKIDKGNGTDYMETIEKQAILSEKHEMIIKQQTTPA
                     KDLVES"
     gene            complement(9962..11356)
                     /locus_tag="KR250_001417"
     CDS             complement(9962..11356)
                     /locus_tag="KR250_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBH0067364.1"
                     /translation="MRMILNKGHRIRASDKNLVYRFSMGTLLFVFVAVILLLNTKQLM
                     RTDWEHFSLLDNGFTLSLYNFITILIATGVCALVAFLYYRFCYDSFKKLLHRQKLARM
                     ILENKWYEADTVQDSVFFTDLQSRSREKIVWFPKIYYQMEKGLLHIRCEITLGKYQDQ
                     LLRLEDKLESGLYCELTDKTLHDGYIEYTLLYDMIANRITIDEVRAENGCLRLMKNLV
                     WEYDALPHALIAGGTGGGKTYFLLTLIEALLHTNAVLYILDPKNADLADLGTVMGNVY
                     HTKEEMIDCVNAFYEGMVQRSEQMKRHPNYKTGENYAYLGLPPCFLIFDEYVAFFEML
                     GTKESVGLLSQLKKIVMLGRQAGYFLIVACQRPDAKYFSDGIRDNFNLRVGLGRISEL
                     GYGMLFGSDVKKQFFQKRIKGRGYCDVGTSVISEFYTPLVPKGHDFLQTIGFLAQARQ
                     DGTATCEAKGDGSD"
     gene            complement(11450..11887)
                     /locus_tag="KR250_001418"
     CDS             complement(11450..11887)
                     /locus_tag="KR250_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004056026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0067365.1"
                     /translation="MCEECYSDENRITPLLNPLDCLENHTQYICGTCGRCICIEHDPN
                     RGLQRWNFPFKSLEIAKLYLRTADYTTKSSCGIYEIENSKGRVSYKIFAGNEDLHLFL
                     KKNKDKKCKQMTPVFNVGEYKEYPHTEVRKLTSDEIKQYMSER"
     gene            complement(11989..12372)
                     /locus_tag="KR250_001419"
     CDS             complement(11989..12372)
                     /locus_tag="KR250_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBH0067366.1"
                     /translation="MRLSNGFVIDKEKTFGELKFTAVRDVFLQNEDGTPSTQLKKRIY
                     DLKCSLHGGIIPVSVPPEVPLREFPYNAVVELVNPVADTVSRKTYTGADVDWYVKAED
                     IVLKNKGNQNTGTSQNHTPQGQPKK"
     gene            complement(12388..12714)
                     /locus_tag="KR250_001420"
     CDS             complement(12388..12714)
                     /locus_tag="KR250_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBH0067367.1"
                     /translation="MEMKYVVPDMAQSFGTLEFAGESEPIFERDKNNRKVIARRSYNL
                     YSDVQKGENVVVEIPVQAGEKHFKYEQKVKLVNPKLYGRGYAIGDVGHTDYVLVADDI
                     VAVEEK"
     gene            complement(12840..15884)
                     /locus_tag="KR250_001421"
     CDS             complement(12840..15884)
                     /locus_tag="KR250_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SrtB-anchored collagen-binding adhesin"
                     /protein_id="HBH0067368.1"
                     /translation="MKKILKRLCTGVLAFATVVTALPSTAVHAESKQYWTESEERVGI
                     VEKVMNDGSIGSTFNEGHLTVEGEDAYCIDINTDFKNGYKTRADASTRMSADQISDVA
                     LSLEYVKQYGEAHKELNYKQVYLLEQCVVWQRLSVHLGWQCDNVRASYDEIPKATQDE
                     VFAGAKAFVKENKGRYECGGYIYSGEGQELGQFWAKLNVGNAKLQKTSSNTSITDGNG
                     NYSIAGATYGVFSDKDCTKQLATLTTDKNGNTDVVEVKAGTVYIRELSAPAGYKVDTT
                     VYSLKVEAGKTATLNVSDTPKVTDTLIELFKIDMETQKDNPQGNASLEGAEFTWKYYA
                     GFYNKDNLPAEATRTWVTKTIAETDSDGTTHYITKLADAYKVSGDSFYMQDGKAILPL
                     GTLSVEETKAPNGYLLDGAYMQAGDKSEQIKGLYLTQITEDGDLAVLTGSNQFSVSDK
                     VIRGGVKIQKRDLETSDTKPQGSATLKDTAFDVISLNDNSVLVEGKLYKKNEVVKTIR
                     TDIEGVASTSADLLPYGKFRIVESEAPDGYLTDGAKPIDFAITENGKIVDLTDEAHSI
                     YNQIKRGDIEGVKIGAGTHKRLADVPFRITSKTTGESHVVVTDDNGQFSTSAEWTSHK
                     HNTNAGKTSEDGVWFGTSEADDSKGALPYDTYIIEEMRCDSNKGFELIPPFEIVVSRN
                     NLVIDLGTLTDEYEKEISIHTTATSKDSEKTILAGKEVTIVDTVKLDGLTKGTKYQLK
                     GWQMLKEENAELIIDGKRVENDYTFIADDEEMKVEISYTFNASELGGKNLVTFEELYD
                     LSNPDEPVKVAEHKDIEDDGQTVLITERIIKIHTTATDKDGNKEIEAGKDVTIIDTVT
                     LEGLEVGTQYKLVGWQMLKEENAELLINGKRVESDYTFTADSETMKAEVAFTFDATSL
                     DGKQLVTFEELYDLSNPDEPKKVTEHKDIEDEGQTITFNEKPEVPEKLETPQTPEKPN
                     RPSDSPKTGDSTNVMAFLVMLLTSAGGLAGTYLYKRRTMKKS"
     gene            complement(16127..17491)
                     /gene="rlmD"
                     /locus_tag="KR250_001422"
     CDS             complement(16127..17491)
                     /gene="rlmD"
                     /locus_tag="KR250_001422"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HBH0067369.1"
                     /translation="MLSKNEMYIVDIVDIGQGGVGVGKYNGITVFVEGGLIHDKVKVR
                     INKVKKNYAVGDIVEIIEKSPFRVKRVCSDNLRDCGGCQIQDLDYNKQLEMKTNEVKQ
                     VLSRIGKLEDVEVHDTIGMDNPYRYRNKAQFPIQKNNGIPIIGFYKKKTHDVISTEKC
                     VIQHEINDKIVKIIKTYIRAYKVSIYDEKTHTGLIRHLVTKIGFTTKEVMVVLVANGN
                     KLPYLKELASVLKENIPGFKTLVVNVNQKDTNAILGRENIVIYGDGKINDYIGDLVFE
                     ISPLSFFQVNPVQTKVLYDKALEYANLNDTDTVFDIYCGIGTISLFLAQKAKKVYGIE
                     IIDDAIKDAKINAKLNNIHNAEFYVGKAEEIVPKMYREGKTANVVVVDPPRKGCDEKV
                     LETIVSMNPERIVYVSCNPSTLARDLDYLNVHGFKCVEAQPVDMFPHSTHIETCVLLQ
                     RRTM"
     gene            complement(17666..19426)
                     /gene="pyk"
                     /locus_tag="KR250_001423"
     CDS             complement(17666..19426)
                     /gene="pyk"
                     /locus_tag="KR250_001423"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HBH0067370.1"
                     /translation="MLNNVKKTKIVCTLGPASQSEEVLTQLMQNGLNVCRFNFSHGSH
                     EEHKERIDMAKKVREKLNKPVAILLDTKGPEIRTGNFEDPEVFLEEGQKFTITMKDIM
                     GTKEMCTVSYKGLAEDVKSGDSILIDDGLVGLRVKEINGEDIVCVVENSGIVKNHKGV
                     NVPGVKINLPAITPKDISDIEFGISQGIDYIAASFVRKASDVLAIREVLENNNATDIQ
                     IISKIENQEGVENLDEILKVSDGIMVARGDLGVEIPTEEMPIVQKMMIKKCNELAKPV
                     VTATQMLDSMIRNPRPTRAEVTDVANAIYDGTDAIMLSGETAAGKYPVEAVKMMATIA
                     KRTEETLDYDRLLKENGTNNVTVTDAISHATCTTAVDLNASAIITSTSSGYTARMVSK
                     FRPKSPIIATTNNEKTMNKLALTWGVYPVKSSVAGNTDEVIEKAIEAARQADYIDNGE
                     LVVITAGVPVGVSGTTNLIKVHVISEEIVQGIGVGTQTVEGKVRIIKNGNACVDFNEG
                     DILVSTATDAEMNNYIEKAGAVVTENGGMTSHTAIVGINLDIPVIVSAADITNLVKDG
                     EVVTVDASRGVVYRGSTRVL"
     gene            complement(19478..20437)
                     /gene="pfkA"
                     /locus_tag="KR250_001424"
     CDS             complement(19478..20437)
                     /gene="pfkA"
                     /locus_tag="KR250_001424"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HBH0067371.1"
                     /translation="MKTIGLLTSGGDAPGMNAAIRAVVRSAIYYGCKVYGINRGYKGL
                     LEEDLTEMNLSSVGDIIHRGGTILKSSRCEEFKTEEGRLKAVKILKKYKIDCLVVIGG
                     DGSFAGAQKLSDLGFPAIGIPGTIDNDLAYTDYTIGFDTAMNTIIDAIGKIRDTSSSH
                     ERVNIVEVMGRHCGDLALYAGLAGGAETIIVPEVEITVDEVALRLKTTQKRGKRHSII
                     VLAEGVGSASDLEKELKKESGADLRVTVLGHVQRGGSPTVSDRILASRLGVRAVELLL
                     DGKSARVVGIKENKIIDLEISEALAQKKVFDKEAYEMAKILSI"
     gene            complement(20646..24215)
                     /locus_tag="KR250_001425"
     CDS             complement(20646..24215)
                     /locus_tag="KR250_001425"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HBH0067372.1"
                     /translation="MNKDFAHLHVHTEYSLLDGFSRVKKLIKRAKELNMSSIAITDHG
                     CMFGVIDFYKTAIKEGIKPIIGCEVYTAARGLRDKDPNYDKYQGHLVLLAKNMEGYKN
                     LIKIVSTSYVEGFYYKPRVDMEELKKHNKGIIALSACLAGDVARALMNRNYEKAKQFA
                     LDYRDIFGEENFFLEIQDHNLPEQKEVNSGLVKLSKETGIPLVATNDVHYVNKEDSKI
                     HDVLMCIQMGKTLNDPNRMRFGSDEFYLKSREEMEELFPYALEAIDNTVKIADMCNVE
                     FDFNTIHLPKYDVPEGYTPETYLRELCFNGLKERYDNPSNEILERLNYELDVIEKMGY
                     VEYFLIVWDFINFSKENNIIVGPGRGSAAGSIVAYTLKITDIDPIKYSLLFERFLNPE
                     RISMPDIDIDFCYERREEVIDYVKRKYGDDHVAQIITFGTMGAKAAIRDVGRVLDIGY
                     NKVDKVAKEIPFALGMTIDKALDTNPNLKELYDQDPETKEIINISKQIEGMLRHASTH
                     AAGVVISKNPVDEYVPLYKHQDAITTQFTMTTLEELGLLKMDFLGLRTLTVIRDTLDL
                     IEKNRVIKNYTEKIDFSKMDYDDPKVYEMLSSGNTLGVFQLESAGMRSFMKQLKPDNF
                     EDIVAGISLFRPGPMDSIPAYIKNKSNPKDVTYLHEKLKPIMEVTYGCLVYQEQVMQV
                     VRDLGGYSYGRSDLVRRAMSKKKMDVMEEERQYFIHGKFDEDGNIEIAGCIRNGVEEE
                     IANKIFDDMIDFARYAFNKSHAAAYGVLAYETAYLKTYYPVEFMAALITSVMGNTDKV
                     VEYIRECKALEIDVLPPDINKSFSKFSVEGDNIRFGLAAVKNVGVNIINNIIAERESN
                     GLFVDLVDLVKRLDQKDTNKRVIESLIKCGAFDNISENRASLMAGYESLLESVSMDRK
                     KNVQGQISLFDAFGDSEEDSDFQQIYSLPKRQEYEERERLNLEKEVLGMYVSGHPLSQ
                     FEKELQEKTSMDNGKLNSLKEEEESYLQMNETDVIMGGMIVTKTIKTTKRNEIMAFIE
                     LEDLYGAIEIIVFPQLLQKYNVILNEDNIIYVKGTLSIKEGENAKLIAREIKDINDDT
                     DFADRNYNSGRTRNVENIKPESGKKLYLKIDSMSDTDTTNNIVEIAKQYPGNDSIYLY
                     PMDENINGKRKAYKMTGALTCICDEFINNLEELLPKDNIKVKA"
     gene            complement(24372..25319)
                     /gene="whiA"
                     /locus_tag="KR250_001426"
     CDS             complement(24372..25319)
                     /gene="whiA"
                     /locus_tag="KR250_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HBH0067373.1"
                     /translation="MSFSTETKNELARVISGNECCNIAELSALVKSGGSIQIVGYKKL
                     NLKITTELNSIARKVFKLLKKNFNINTTISVNKNQMLKRNNSYVLMVTSEMGSEMLLK
                     KLGILEDKEGFFTINKISENLIKHEGCKRAFIRGAFLGGGSISDPEKNYHMEFVTNNE
                     EFADSLKELINSLGFNSKIVARKNNHVVYIKESEQISDLLSIIGGHHALLSLQNTKIV
                     KEMRNNVNRIVNCETANLSKTVNAAVRQVENIKLIQETIGISSLPENLQEIAKIRIEY
                     EDMTLKELGEMLEPPIGKSGVNHRLRKIEEIATDLKKKS"
     gene            complement(25336..25761)
                     /locus_tag="KR250_001427"
     CDS             complement(25336..25761)
                     /locus_tag="KR250_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBH0067374.1"
                     /translation="MREEVSAGGVVLFGNTILLLRKFNGDWVLPKGKVEEGENNQEAA
                     LREVSEETGVKADILKYLGEIHYTFKENWDENRAVHKTVFWYLMQAKNMDTIPQKEEG
                     FIDAKFIHLDRVVDLARYDDEKEIIKVALQEIKKRLKKN"
     gene            complement(25830..26936)
                     /locus_tag="KR250_001428"
     CDS             complement(25830..26936)
                     /locus_tag="KR250_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HBH0067375.1"
                     /translation="MAKLLIIVGILGWIALIVSLFIYKKAKNEKVKLFQRSIVENREP
                     NVVVIGGGTGQSVFLRGLKHTTQNITAIVTVADDGGGSGVLREDLGMLPPGDIRNCLL
                     ALANIEPTMNEVMQYRFTEGLLKGQSFGNLFLAAMNGLYGNFEKAVYKLSEIFAITGR
                     VLPVTLEDVNLVAKLENGNIINGESSIPEESKIQKSSIDKIFLNPKDVKPLKDVIASI
                     YDADIIIMGPGSLYTSIIPNLLVEGIVDAIKSSVAPKVYIANIMTQPGETEGYNVLEH
                     VNAIVKHTDENLIDYVIANNEILPEEMLDLYKQDGAEQVLLDKKQKDKLKEMGIKTVE
                     KNLIEIKNNYIRHDAKYISNIVIELALNHNYNKS"
     gene            complement(26948..27805)
                     /gene="rapZ"
                     /locus_tag="KR250_001429"
     CDS             complement(26948..27805)
                     /gene="rapZ"
                     /locus_tag="KR250_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HBH0067376.1"
                     /translation="MKFVIVTGLSGSGKSETMRALEDMGFYCVDNLPPALITKFAELC
                     YQPNSSIDKVALGIDIRGRKFFEALHESLNYLEKENYEYEMVYLDCNDDVLLKRYKMT
                     RRNHPLAKDMQIPEGIKMERKIMEPLKELSTCIIDTTNMKPKDLKEEIKKIYSSGEDN
                     PNLTISVVSFGFKHGILADADLVFDVRFLPNPYYVEELRAKTGDDKEVRDYVMNSKIS
                     EEFYVKLLDMIHFLVPQYIEEGKQHLVIGVGCTGGRHRSVTITNLIAEDLSNKGYRVV
                     KKHRDSMLR"
     gene            complement(27836..28549)
                     /locus_tag="KR250_001430"
     CDS             complement(27836..28549)
                     /locus_tag="KR250_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HBH0067377.1"
                     /translation="MKILADYHTHTLYSHGKGTIEDNVKVAISKGIKTIGISDHSYKH
                     IAYGVKKKDILKMREEVDYLNDKYTDINILLGMECNILDDKGNIDMDDKFIRDFDYIM
                     AGYHFGSTPTSLRSLLNHCNNYIFKTEKAKEYNTKAVINAISNNDIFIITHPGDKGDV
                     YIEEVAKTAKKTNTRLEINSSHSFLNVNQLNKIKGLENKFIIGSDAHCPERVGDFELA
                     MKTISDSNLDISLVENIII"
     gene            complement(28553..29467)
                     /gene="murB"
                     /locus_tag="KR250_001431"
     CDS             complement(28553..29467)
                     /gene="murB"
                     /locus_tag="KR250_001431"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HBH0067378.1"
                     /translation="MNNQDIYINLLNILSKEDIKVDEPMKKHISFRVGGPADILVRPR
                     TEEQLKNVLKLVKEESIPYLIIGNGSNILIKDGGIRGVVIELADNFNSYEINDTRMTA
                     QSGALLSVLGKALQKQELKGFEFASGIPGTLGGALAMNAGAYGGEMKDIVKSVRLMDM
                     EGNIFELSNEQMEFGYRKSIISKNGYIALSAELELQEGNYDEIKSLMDDLATRRITKQ
                     PLNFASAGSTFKRPTGYFAGKLIEETGLRGLTLRGAQVSEKHCGFVVNQGEASAKDIL
                     DLIYVIKSAVYAKFGVMLEEEVKILGED"
     gene            complement(29740..30510)
                     /locus_tag="KR250_001432"
     CDS             complement(29740..30510)
                     /locus_tag="KR250_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HBH0067379.1"
                     /translation="MSKGFLTPGETAQATISAGIKKANISTQNAILLGLFGGAFIGFG
                     ALGSMIVSQTFGKIDPGVASFLSAMVFPVGLMLVVVAGAELFTGNTLMAIALMDKKIT
                     LGKMLRNWGLVYFANLVGSILLVALVFYSSTLTGDAATKAIAVAEAKVNSTIASMFLK
                     AILCNILVVLAVWMATASQDIMSKLASCWTVIMLFVLCGFQHSVANMFFIPMGMALGA
                     NITMVQFITNLVFVTLGNIVGGSIVVGGIYYLCYVKNS"
     gene            30828..32309
                     /gene="cls"
                     /locus_tag="KR250_001433"
     CDS             30828..32309
                     /gene="cls"
                     /locus_tag="KR250_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HBH0067380.1"
                     /translation="MFDISFFEMSIYEIAVTTIYIINFMVILNLIFREKRNINTTLTW
                     LLILVLIPALGFILYIAFGRNISKNNMFRLKEKDDKIIKSNILDTQVKLQSTSEIDSD
                     IHQHKDMIYALANSNNAHYTNNNDVWIYAESSQFFNSLLEELKKAKKYINIQFYIFKD
                     DKIGTEIIDILIDKAKEGVEVRLLFDAVGGRTLKNSTLSRLKESGVKVGSFFPSFLKI
                     VNFNLNYRNHRKIVVIDGKVGFVGGYNVGDEYLGRNPKFGLWRDTHTKLTGDCVIDLN
                     MRFILDWRYTTKEDLDLEKYFIEEDTNSNHPSENIGIQIVSSGPDISELDEIKYGYLK
                     MIQKARKYIYIQSPYLILDSTFIDTLKIACLSGVDVRVMIPSKPDHPFVYWASYSYAG
                     ELLKFGAKIYTYGQNAFLHAKTIVIDDSICSIGTANMDIRSFELNFEVNAFMYSSKKA
                     LEQRIIFENDILNSKEITLDIYNSRSTYIKIKESISRLLSAVL"
     gene            32627..33124
                     /locus_tag="KR250_001434"
     CDS             32627..33124
                     /locus_tag="KR250_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HBH0067381.1"
                     /translation="MCDFISHNKQLFDELDIFIDSLATKEGALIQVLHEAQGIFGYLP
                     KEVQLHVARKLGVAPAKVYGVVTFYSYFTTEPVGKYKISVCLGTVCFVKGADKILSAF
                     EKQLGIKVGETTSDFKFSLEGLRCLGACGLAPVVTVNGKVYGKVKPDQVSEILDTYRE
                     LELNC"
     gene            33141..35027
                     /locus_tag="KR250_001435"
     CDS             33141..35027
                     /locus_tag="KR250_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HBH0067382.1"
                     /translation="MRKVNSFDELKVLADELKPNLSLRKNYSKENVLRRELLVCCDTG
                     CTSSNSLEIVSELENEIKKSGIQDKVSVRLTGCFGFCAQGPIVKVYPDNVFYVKVEPS
                     DAEKIVQSHLIRNTVVEELLYEEESLSKKVEEQEEMSFYKKQLRIALKNCGLIDPDSI
                     EEYIANDGYLALGKCLTSLTPQEVIAEVKTSGLRGRGGAGFPTGSKWEAASKNPAGPT
                     DKKFVVCNADEGDPGAFMDRSVLEGDPHSVLEAMAICGYAIGSDTGYIYIRAEYPKSI
                     ERLKVAIAQAEKYGLLGENILGTGFNFKLELKYGAGAFVCGEGTALMHSIEGRRGEPR
                     MKTYSSSKSGLWKSPTCLNNVETFANIPAIILKGGDWFANLGTEDSSGTKVFALGGKV
                     ENVGLVEVPMGTTLRDIVFEIGGGIPEGKKFKAVQTGGPSGGCIPTEHLDTPIDFGSL
                     SSIGSMMGSGGMLVLDESDCMVDIAKFFLEFTVEESCGKCTPCRIGTTRLLEILTKIT
                     EGNGTLQDLDDLENLAETIQTASLCGLGKAAPNPVLSTLQYFKDEYIAHVVDKKCPAG
                     KCQNLLSYFITDDCKGCTKCSRVCPAGCITGSVKEQHTIDTSKCLKCGACIDNCTFNA
                     IIKK"
     gene            35050..36831
                     /locus_tag="KR250_001436"
     CDS             35050..36831
                     /locus_tag="KR250_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase small subunit"
                     /protein_id="HBH0067383.1"
                     /translation="MSLVNLTINGKHVSAPSGTSILDAAKLINIKIHNLCHLHMNEID
                     KLDTCASCRVCMVETERGLVPACGTVIKEGMKVQTNSAKALNARRTIVELLLSDHPQD
                     CFVCEKNGDCELQTIAADLGVRKIRYQGSKSFAGKDTSTKSLVKDHSKCILCRRCETV
                     CNDIQTVGALSGVNRGFNTLVSTFFNSDMVETECTFCGQCISVCPTGALTEVDNVPKL
                     WDVLNKKEKTIVVQVAPAVRVAIGEEFGLEPGSISTGKMVAALKALGFEHVFDTNFGA
                     DFTIMEEATEFIERIQKGENLPILTSCCPAWVNFLEHNYPDKLNLASSCKSPQGMFGS
                     IAKNYYAPKILGINPEELYVVSVMPCVAKKYEASREELSESGILDVDLSITTRELAKM
                     IKEAAIDLPNLQDQDFDNPLGKSTGAASIFGASGGVLEAALRTSYEKITNKTLDNVNF
                     TNVRGLKGIREASIDVDGTTVNVCIVNTLKNARKIMDKVRSGECKYHIIEVMACPGGC
                     VGGAGQPYHHGNTEIVDRRANALYEIDRNKAIRKSHENPDLQAIYKDFFGEPNSDVAH
                     KYLHTHYFDKSCVYGECPQECACEEAK"
     gene            complement(36971..38767)
                     /locus_tag="KR250_001437"
     CDS             complement(36971..38767)
                     /locus_tag="KR250_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate oxidoreductase"
                     /protein_id="HBH0067384.1"
                     /translation="MGIEYFSKLLESSTEKELLLSKKSNNISTARLISFLIVIAGFAI
                     GFYNKNIVGIFVGVLSIIIFIALLVIHSKVKEEETYFKSKSEVLNKYIKRFGDGWKEF
                     KIDGKEYLKDENSQAKDLDLFGRASLYQYICVANTSYGKNFLAKYLWNENPDENVILE
                     RQRAIKELLSKDDFSIHIQTLSNIIGKEQKNNSDSSIESFIEYGENKNVYIPKWMHIF
                     TWGLPTATILSFIFCMLGFLPVLPVFLLFIIQLGFSGFGYPRLMQTLAPLFSFSRSIQ
                     VYEKMFEVLEKETFESAYLKELQDKLSKGSGVSRGVKQLNSIGNAVNLRYNQILYIIA
                     CGVLMWNYHCAEALERWKGIYGNQIRDWFESIGEFEALISLTVVSHVKENTCFPVIKY
                     EDTPRLKVEEVYHPLIAEKSVIANSIQLNSQTCIITGSNMSGKTTFLRSIGVNLVLAY
                     AGAPVCAKSFDATCMAIFTSMRIQDDVSQGISTFYAEILRIKSMIQYSLKELPMLVLV
                     DEIFKGTNSADRIIGASEAVKKLSKPWVISMVTTHDFELCDLSGSGDVEVVNYHFSEY
                     YVDDKIHFDYKIKDGRCKTTNAKQLMRMAGIL"
     gene            complement(38981..39910)
                     /locus_tag="KR250_001438"
     CDS             complement(38981..39910)
                     /locus_tag="KR250_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HBH0067385.1"
                     /translation="METSNKVSIREIIKELKLKVVYMPDDVDYYVYSQDINRPGLQFA
                     GYFDYFAYERIQIVGKTEYNFFGLMDSHIREEVLDRFFSYEIPALIISRDLEIKPDVI
                     EKAKKYKRILLSTNRNTTRLINRLSNYLDNKLAPHTTIHGVLVDIYGIGVLIKGESSI
                     GKSETALELIQRGNRLVADDAVEIRKLDESMLVGQSPELIRHFLEIRGIGIIDVRSLY
                     GVGAIKNSKMIDLVVHLEAWDETKYYDRLGLDKEYEEILGVNVEKLVVPVKPGRNTAM
                     ILEVAAMNFRQRGMGYDAAQEFTKKLSKLIDTK"
     gene            complement(39910..41727)
                     /gene="uvrC"
                     /locus_tag="KR250_001439"
     CDS             complement(39910..41727)
                     /gene="uvrC"
                     /locus_tag="KR250_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HBH0067386.1"
                     /translation="MFDIQEHLKKLPSEPGVYLMKDKYDHIIYVGKAISLKNRVRQYF
                     QSSKNHTSKVKSMVKNIYKFEYIITDSELEALILECNLIKRYRPKYNVVLRDDKTYPY
                     IKVTTNEDYPRILKVRRVLKDKAKYFGPYTNITAVNDTLELISSTYPIRSCKIDIDKA
                     IKNKTRPCLNLHINKCLGPCTGNVSKEEYGKMIEEIIMCLSGKEEKLMELLKEKMNES
                     SMNFRFEEAAVYRDKIKSLEEMIQKQKIDATVSDLNQDVVAMARAHNEACVQVFFIRN
                     GKIVGREHFILEGVMDSPRASILSSFVKQFYNEQEYIPKELIIEDEIEDSSILEEWLS
                     SKKGQKVTIRVPQKGEKKSLVEMVRKNAVEYLEKFSDMNKRKYEKSIGALEELKQILN
                     LEKLPIRIEAYDISNIQGVDSIGSMVVYTNAKKDKKEYRRYKIKTVIGPNDYDSMAEI
                     VDRRLKHGNLPDLILLDGGKGQVSAVKKVLELNDVDIPLWGMYKDDKHRTKGLICKEK
                     EIELDKTTNLYRFIASIQEEVHNYAITYHRSLRNKALTKSILDDIQGIGEKRKKSLLN
                     HFKDVDAIKKATMEELLEVDGMNKSIADNVYNFFRKEEN"
     gene            complement(41986..44811)
                     /gene="uvrA"
                     /locus_tag="KR250_001440"
     CDS             complement(41986..44811)
                     /gene="uvrA"
                     /locus_tag="KR250_001440"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBH0067387.1"
                     /translation="MEDKIIIRGAKEHNLKNIDLELPRDKFIVFTGLSGSGKSSLAFD
                     TIYAEGQRRYVESLSAYARQFLGQMEKPNVEYIEGLSPAISIDQKTTSKNPRSTVGTV
                     TEIYDYLRLLFARVGDVYCPTCGEKISQMTIQEIVDKMLDFPDRTKLQILSPIVRGQK
                     GTHKKVFDNIKKEGFVRVRVNGENYEVSDDIILNKNQKHNIEVVVDRIVIKDGIESRL
                     ADSIETAVKLSDGLVIAQIIDGEEILFSTKFACPEHGIGIEELSPRMFSFNAPFGACD
                     VCNGLGESKEVDPELVVPNKDLSIKQGAVAAWGSTGVNDDTYYSKMIKSLAEHFNVSL
                     TTPFKDLPDDFVEELLYGKDNIIVEFTYESKFGGTRNYKSYFEGVIVNLERRYRETNS
                     DYMRDKIEEYMAERPCPKCKGMRLKKEVLSVLVDGKNIMEVTNLSVNELIDFMENINL
                     TEKQRFIAHEIIKEIKGRAMFLRDVGLDYLNLSRKAGTLSGGEAQRIRLATQIGSALV
                     GVLYVLDEPSIGLHQRDNDRLITTLRHLTDLGNTLIVVEHDEDTMREADYVIDIGPGA
                     GIHGGEIVAQGTLDEIIENENSITGQYLSGKKQILLPETTREGNGSFIEIVKASENNL
                     KNIDVKFPLGKFTCITGVSGSGKSTLINDILYKGVASKVNRLKQRAGKHKEIKGIESI
                     DKVINIDQSPIGRTPRSNPATYTGVFDFIRDIFASTNEAKARGYKKGRFSFNVKGGRC
                     EACKGDGIIKIEMHFLPDVYVPCEICKGERYNRETLQVKYKDKTISDILDMNVEEAVE
                     FFENIPNIKRKLETLMDVGLSYIKLGQPSTQLSGGEAQRIKLAAELSKRPTGKTLYIL
                     DEPTTGLHMADVDKLIQVLQRLADTGNTIIVIEHNLDVIKTCDYVIDLGPEGGDKGGT
                     IVATGTPKEVSKVDGSYTGQFLKKYFS"
     gene            complement(44830..46800)
                     /gene="uvrB"
                     /locus_tag="KR250_001441"
     CDS             complement(44830..46800)
                     /gene="uvrB"
                     /locus_tag="KR250_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HBH0067388.1"
                     /translation="MDFKIKSDFKPTGDQPEAIKSIVDSINRNEKFSTLLGVTGSGKT
                     FTMANIIQQVKKPTLIMAHNKTLAAQLYSEFKEFFPDNAVEYFVSYYDYYQPEAYVAH
                     SDTYIEKDASINDEIDKLRHSATASILERRDTIIISSVSCIYGLGDPKDYKELMLSIR
                     PGMQRDRDDVIKRLIEIQYERNDINFTRGTFRVRGDILEIFPASNDEKAIRIEFFGDE
                     VDRITEIDYVTGKIVGTRNHVVIFPASHYVTTPERIEKAIVEIENELQEQIKFFKEND
                     RLLEAQRIEQRTKYDIEMLKEIGFCQGIENYSRHITGRSEGERPYTLMDFFPDDYLII
                     VDEAHVTIPQVRGMYAGDRSRKTSLIENGFRLPSALDNRPLNFQEFEGNINQMLFVSA
                     TPGPYEIQHSETIAEQIIRPTGLLDPIVEVRPINNQIDDLVGEITKTIEKNERVLITT
                     LTKKMSEDLTNYLKEIGIKVKYLHSDIVTLERTEIIRDLRLGKFDVLVGINLLREGLD
                     IPEVSLIAILDADKEGFLRSETALIQTIGRAARNENGRVIMYADRITDSMQNAIDETK
                     RRRDIQNLYNEEHNIIPKTIQKNIRDSIEATKVAEEEVVYGISDTDDKDEIRANIDKL
                     KSEMMEAAQNLQFERAAELRDKVKQLEEKLEK"
     gene            complement(46987..48249)
                     /locus_tag="KR250_001442"
     CDS             complement(46987..48249)
                     /locus_tag="KR250_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HBH0067389.1"
                     /translation="MTKVIVLSVDSLFEKDLEFVKNLPNFKSILENCSIVKNINCVYP
                     TLTYPCHTSMVTGVYPKKHGIYHNEKFDPNKENKDWYWYSKDIKAKTIIDVAKENNLT
                     TSSVLWPVMGANPNIDYNIAEIWAPSREDDPRETFERSSSKVIMDGIYNRHCHYIDWK
                     LEPNMDLFGVNCSVDIIKEYKPDLMLIHLATLDHTRHNYGLFNDEVDKALKMNDKWLG
                     DIIQATKDAGTYKDTNFIILGDHGHLRVDKVINPNVLLKSNGFIKVENGEVKDFDAYV
                     NSSGISAQIIVNNLDRLTELRELLEEFKEELFIENIFTKEEASNLGLEGDFEMVIEGL
                     EGISFGNDFEGSIIKDSDIKDYKFAVATHGHLPTKGNRPPFIAFGPNIKGGVVIEEGD
                     LRAHAATILKMFNLKLDGVEKEAFDFIM"
     gene            complement(48312..49616)
                     /locus_tag="KR250_001443"
     CDS             complement(48312..49616)
                     /locus_tag="KR250_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBH0067390.1"
                     /translation="MKLKRKLATGLAITLLLGSLVGCSNSNDANASKGEKTDSNITKI
                     DFWAPLGGTNGETAQKMVDQFNSEHKDVQVNMLKQKDYYENATKLQAALTSKDQPDVT
                     LLEITQTGTFASAGALVDMSKYFDKTYQERFFSGLLTNSYYEDKFVGMPFNRSTPILY
                     INKDMATKAGLDPSGPKSWEELKDYASKMTNKSEDTYGFETPIDIWFYEAMVMQSGGE
                     ITNGKKVAFNNEAGQAPVKFWQDMMKAGIMKMPPGEDYNAWDVAKQDFVNGKVGMIFT
                     STADLAGLMQQTEGKFEISTAFLPKNQKYATPTGGANLVMLEGGTDKEKEASAEFIEW
                     MTQTDKIVQFSSSTGYLPTTEDATNSEKLQTLYKEKPQYKVATDQLQYAVALPMLNGY
                     KEATDKLMDEIKKGLTDLNANPKDVVSKGTSAMQAVIDKTNK"
     gene            complement(49647..50507)
                     /locus_tag="KR250_001444"
     CDS             complement(49647..50507)
                     /locus_tag="KR250_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBH0067391.1"
                     /translation="MDIIQPIKKDKVDIKKIASKSIEILILLLLSAIFIFPFIWMLST
                     SFKNPQEMMQLPPTIIPNDIVLENYSTAWHSGPFFRYLLNSVFVTGSVLIVQFLVMVP
                     AAYAFSKIKFKGEKMLFGLLLVGLMIPPQVTFLPVYMMMSKFGFINTYVPLIIPFMTS
                     SFGIFLLRQNFMQISDEIIEAAKLDNASDLKIMFRIMVPMAKPAVITFILFNFIYHWN
                     NYFWPLVMTNTDAIRTLPIGVALLKSSEGITAWNVIMAGNIILILPIILVYIFANKKV
                     KEAFMYSGIK"
     gene            complement(50551..51420)
                     /locus_tag="KR250_001445"
     CDS             complement(50551..51420)
                     /locus_tag="KR250_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBH0067392.1"
                     /translation="MKKQFNKAYLYITPAIIGTLLFIVYPIIKTISLSFKSGSLLSST
                     FENVGLNNYTELLKNPEFIQTITNTAIYTFFMVTISISLAIVLAVWLNKNSIIHNLTQ
                     SIIFTPHVISLVSVAVLWLWIMEPQYGFLNWILSTLHLPTSKWLSSESSALMSLILIG
                     IWKTLGYNILIVLSGLKSIPNYIYEASKLDNANKITNFFKVTLPLLSPTIFFLMITTT
                     TASFQVFDEISIMTQGGPVGSTNMLSYYIYESGFIYYDIGQASAASMFLLAIVMIATY
                     ANFRLLSKKVHYQ"
     gene            complement(51404..52474)
                     /locus_tag="KR250_001446"
     CDS             complement(51404..52474)
                     /locus_tag="KR250_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0067393.1"
                     /translation="MADIRLENVTKSYNKKNTVIENLNLTIEDGSFTVLVGPSGCGKS
                     TTLRMIAGLEDITSGRLKIEENDVTKTDASKRDIAMVFQNYALYPHMTVRENIEFGLK
                     NKKVEKEKREKLITEVIDVVGLREYLNVKPGNLSGGQRQRVALARAMVKNPKVFLMDE
                     PLSNLDAKLRNQMRTELIQLHQKLKSTFVYVTHDQVEAMSMADKIVIMNKGKIMQIGS
                     PKKIYQNPKNLFVAQFIGSPCMNILDIKNDIKFGFRPEKATLESINIDGKNDDVFSLF
                     GKVVTKEVLGNETIYCLSVLNREIRVKTENDIFDIGRTLRISVLEKDLYYFDKNGERI
                     EDVKVCKSMYSKIKGDIYEKAI"
BASE COUNT        15511 a        10360 c         7536 g        19510 t
ORIGIN      
        1 ataccgacgt gggttacata cgaaccagcg ttataggtgg aatggaaaaa taccaaatcg
       61 ccagcctttg cctgcgagag tggaagatgt tgggtagcgt catactgggc ttgtgccgtt
      121 ctcggtaagg agataccagc ttttccatag caccattgcg tcagtcccga acagtcaaag
      181 gaagtggttg gagaagaacc accaaacaca tatttccacc cttgatattt caacgcttca
      241 ttcattacct tttgtgccag ctctcccgat acctgcggaa cagttaaata ctgattgact
      301 aattccacat agaacatatt tccatagcca taccgccagc ctccgttctt cgcaacggct
      361 atcgggttgg tataggttac tttctttcca cccgatttct cacgggcgaa gctctctgca
      421 agattaaaag tgtgtttctt tccgtttcct gccacatagc ccacatagcc accgccatag
      481 ttataggact gtatcgctac attcaaatcg tcgatatctt gatttttgca ggaagaaagc
      541 agggacgcaa aatacttaca cccctgcttg attgagcttt ccgtatctaa ggaactaggc
      601 ggtaaaccaa gactttctga actctgcata acatcttctg ccgtaccgcc actttctact
      661 tgaatgatag ccagtagtac attgacgtac tcggagatac cgttttctct ggcgtatttt
      721 tctaccatag tctgatgttt caaaacttct gcggataagt tcatacccgt aatgccagaa
      781 gaaaagttgc tgttttcgtc gtcgctgtcc gcactaatca gcaccccaaa gaaaagcacc
      841 agagaaaaga gaataggaaa cagacttcca atgatagtga tatgtttcag tttcattttt
      901 tcttctgtcc tttcttcgtt acaagattac ggattttctc tgtggtctgc tggttcttct
      961 ggacagtctg ggtctgctga acctttgtcc tacggtcttt ggtgtaattc tggcgtgttt
     1021 cctttgacac ggtaactttt tctacattct gcctatatgt cgattgggtc gctttcccgt
     1081 caggagtact agaagatagc ggacgttctt tgacaccgct tgtcttaact ggttcgcttg
     1141 ctttcgtagt cggagttgtg gcagggcgtt tgacttcctg cattttttcc gcacccgtct
     1201 ttgaaacggt tgaagctgtg gctggtcgct catggggacg ggtagctccc gttgtcgctg
     1261 atccgtcagc ctttcgctgt gcctgccttg cttcttgtgc cttttgaagc tccatacgct
     1321 tgacagcgat acttttctta tgctgttcct gcttttccaa gcgtcccgtc tgtctggact
     1381 gctgttcctg caccatacca cgcttgaaat cagacacgct ggactttgcc ttttcctttg
     1441 cggaatacac cgcataagcg gtctgtgtgg gcatatcctt gatattctct ttgacagcgt
     1501 ttgccttgtc tttcacttta tttttcgtat ctaagacagc acccaccttt gagcctgcac
     1561 gctgtcccat actggaagtg gtattgcccc gattttcttt agaagccgta ttttttcttt
     1621 cggctctctt gccagcaacg gcacttccag ctaccgcacc agccacaccg cccgaaatac
     1681 cgccagcact tacagccctt gcaatacggt gttccatacg cctagcccga tgtcgcatga
     1741 acagatacgg tctgcggaat atcctgcgtc ccatgctttg actgtcgcca gcgttgagag
     1801 aaaacatact cattaagtcg cccagcttca tatagatacc agcgaaacat actatctgca
     1861 agaacgccac catgaaaaac ggatagtccg tggatatgtt ataaaacata ctggaaatgc
     1921 taaacgcaac cgttacaatg agcgttattc ctgcccgtgt cattatggta ttaaataccc
     1981 tcacgattgc ctgctttgcc atgctttcat agctcggtat catggaaagt agaaagctga
     2041 taggtaaaaa catagcgaag ataataaaaa gtatctggct gaacaacatc atacccgtaa
     2101 gcaagaatac aaatatcgta ataccaagat taaagaacaa gaggaagaac accataccta
     2161 aacggtttac cacctgcggt atcgtcagat tgttgttgtc gttgtcctcg atttctgttt
     2221 tcacgacttc ttctctggtc tttccgtcct cgtcctctgg acttgccgat acgagagctt
     2281 cgacacggtc tgtcccgatt tcctctgcgt tgctgtttcc aaactgtaag agtagccacg
     2341 gctgttgtac ttgaatagaa aataggctgt cccgtatcaa gtctacgctg tccttgccct
     2401 cgctgtcaga gttggggagc atgatttttg ttcccaaatc aagcgaagcg gtactgatgt
     2461 ctgatgaaaa ttcatttatc ttcttgatgt agtcgggagc gtaggcaata aacgaagcgg
     2521 acagcacgaa caccaccaca aagttgataa cagcgtgaag tgccttgctg gtttcccgtt
     2581 ttatcagtcc cgtataggca acatacattc ccaccactaa gataataaga agcaggaaac
     2641 caacatagaa gcccgtagaa gaaaagccgt tctcggtaac gcctgcaagg gtctgtatgc
     2701 tttttccgat actgtctgcc atatcgttaa taaaatcaag tttataggct tcctgcacca
     2761 catagccagt tgcattgctt aaataaaggc ttatcgtcca gacaaagttg gtaatgcagt
     2821 aaagcccgta ctgtaccgat tttccgatac cgtccagcca gttccacgga agccacgacc
     2881 agctattatc gacgaaaaaa tcaagctggt agttggaaag cggatatttt gaatatagat
     2941 tttctgcgtt tatggtatcg tccacaagcc ccgtcgcatg agccaccgtc cccagaagtg
     3001 aaagcaggat aagggaaagt gccaccacga acagagccat tttgagaaag tgaagtatct
     3061 tcctttttgt gaacgcactc tgtatccttt ctttcatcac tccacctcat ttctctgtac
     3121 gggcggtcta gtatcaaagg cgtgtagtaa ttcttcaaag actgggtgta tctgcaccac
     3181 accgacacgc ccgtataagt cctgcaataa acattgtccg ttctccaaat cacgcaagcg
     3241 tttctggttg ttttcgtcgt ccttgtcgat accgaaaaat tctaaggtct gctttatctc
     3301 gttgatgtca gtagaacgaa atgcaaactt caaaccgata ttgtttttca gactttcctt
     3361 tgacacgtca taggcagact gggtaacgaa ataaacgcct gcctgcatag ctcgtccagc
     3421 acgaaccagc ttatttgata gggtttcgcc ttgtgccaca tttaagaacg cccacgcttc
     3481 gtccaaatct acaatcttaa aaatgcttct gtccgaatgg ataaaatcaa gggcaaaggt
     3541 actaatcaca atcagcatag acaccgacaa taattcaatg gtcgtgtatt cctcaaaagt
     3601 ggtatcttta tctggcagta caaggtctgc tacttgaatg atattgagct ggttatcaag
     3661 gcttatagca ttttctaccg tcccgtctga aaacagcagg tgtgcaaagt cgtagtccgt
     3721 gaagctgtcg atatggtctg cgatattgcg tgagatagcg gtatcttctt ttctcaattc
     3781 ctcaataacg tgtaacagtc ctctattttc actctgggta acagaacgca ccgcttttcg
     3841 taacacgggg aatttttcgc cgtccctaga ggaaataccc gtaaggaatg ttaagatgtc
     3901 gattgccaga ctttcagcgt ctttcacatt cttcataatc acaaatgggt caagaagtcc
     3961 tgcattgtcc ttgtcgctgg taaggtttac gatattgatt tcatgggcta tctctgggag
     4021 cgtttctttc cagttgccac gctcactttt agggtctaac agaagtgctt gaccgccaaa
     4081 cagcaccgca taatatacaa gtagattgtt gcagaatgac tttccaccgc caagacttcc
     4141 cacaaaagca gaagcaaggg cattagtaac tgtgcctttt actccctgcg aagctaaaga
     4201 cggttgcagg tacacatttc ttcccgtatc aacggaatag cccatataga tacccgtgtt
     4261 ttcccctaac tgctgggtcg caccaaaacc aagcccagcc aagaagtctg actttacata
     4321 ctgcacatag tcattgatat atcgcttgct ggcaggtaaa aattcagaat gaagtcccaa
     4381 catatcgcca gcaggacgca ccagttttac attgaggtcg tcgtaaaagt ccttgacttc
     4441 atcacaacgg cgtttcagct cgtcaagatc gggtgcagac acccgtatga cgtagcttaa
     4501 cttatacata ctttctttgc tctggtctaa atcggtttcc agctcgtcca cgctgtctaa
     4561 agcgtccacc acatttgagc tggtttcact tcctgcttga taggcgtgat tatccaaatc
     4621 tttcaattcc tttttcttgt tgcggactgt cgttaatgcc ttacggttct ccacgatttc
     4681 tacattcata gaagtatcaa cggggaatgt gaattgctgt tgctggaaat agaagatttc
     4741 agaggacgga aaatccaact ccccgacaat tacattgacg gtaaaatagg acacatagct
     4801 ttccttgtcc tcatgttcca atcgtaaata ccgctggctt tcctcaatca cacaccttgt
     4861 cggacggata aggtcgtagt attttatcag cgtttctttc ttcaatttct tctttggtag
     4921 ctggtactca taatcttcat aggcgatacc gtccctgccg taaagatgtt ccatgagata
     4981 cccaaaatca tggatttcca agcgacgcac cttaaagcga cgggagattt tattttccag
     5041 taacttttcc attttcatgt aacggttgat ttcatcattc ggcatggaaa caaaatcatt
     5101 catcagcgtg tggttcactt catgaagaaa ttccttgaac gtcagccatg ccgatttttt
     5161 gatgttcttc agattgagct gttcttccgt aaccatgagc ttaaagccaa gaaaaaagcg
     5221 gtagtccact tgattgtccc caatcattga tactaacgct tcggtctgtt cgtctatttt
     5281 ttggactgcc acctcacgaa gtttgcctgt taccagtttc tttgactgct cctgcatact
     5341 tctaatggag ctttctgtgg caatctgcaa cgcatgaatt tttccctcac gggactgtgc
     5401 gattagctgt cgaaaactgt catgcacgat aaatttctgc tctgcggata gaaaagaata
     5461 gttgtacggt atcatctcat agtaggcgaa cacctcattg tccttgttcc agacaaggtt
     5521 attgtcaata tattttatcg ggaacatagt tcacactcct tactgccgtg attgtttcat
     5581 tcaatatctg cttatgcagt tttacggctt ttcctgcata agtgatttta ggtcgcaagg
     5641 cataggttat ctgtgatttc aaaaagctgt acggcttctt tccgtcaaag gttttctgcg
     5701 acataaacca agtgagagca acgggaatac cgaagtattt gagaaatgct ccctcaatca
     5761 tggaaagtgg gggcatatcc ccaaacagaa tgatgataaa ttccgtaatc acaaaccatg
     5821 taatctgtgt aaaggtaacg ggaaagggta agttaaagtc attgattgca tataagactt
     5881 tttccacgtt ccagataccc gtgtagcttt taatcttttt caagttcttt cagccccttt
     5941 cgtaagttct tcaaagagaa aaggacagcc attttcagac tgtcccttaa agagaaatac
     6001 gctgtcagta gcaacaatgc ttgttgctga tcttccagcg ttaatatggt atctcacaga
     6061 tacctcggct tgtttcaatg aatcgaccac ccatatccaa atctcgcccg taggcttcat
     6121 agtcaatata gttctgtaag ttagctggaa tgtccccaag tgcgttcaat tcgtctatgt
     6181 agtagtaggc aacatctgtc atggtttcac aatcgggata aaaataaata tcgtccttat
     6241 gttccacgac ttcttccagc gtcccatagt ggctgataaa ttcgtccaga cactctacga
     6301 tatagtcggg aagttcctct atcatttcat acatttcatt gagttcttca atggaaacat
     6361 attcccctat gtcgatagga aagttgtcgg tatcatggac agcgtattct tcgtactgtt
     6421 cattcaagcc gattttttct tttacatctt cctcgtcaat agggaaagtg aaccacgcac
     6481 cgactaaata tccctcattg tatctgccaa gattagcaat ataaaccgcc atatcatcaa
     6541 tcataagcac cacctcattt cctattctgg cagttcaaag atactgtttt ccgttacaag
     6601 atagcgtttg ttttcaagta agtgagaagc gtaggcttca aaatcgaaat agtatgcttg
     6661 acagtcctcg gataaatgtt tgaatgtcgg gtcgttcatc agcttttgcc ttgcaacatc
     6721 tatcatgctt ttacagtcgt gataaacggt aatcacgttc ctgcaaatat aaagtgcttc
     6781 taagttctca aacacggtaa gaagttctac atattcttcc tgcatatcgg ctgatagctg
     6841 gcggtacata aaatctaatt cgttaagctg gtacacgctg gtgtgttcgt ggacttcatc
     6901 agcataaggc aacacctttt cgataatgcg gtaatcttga ctttctgcac cgacacctaa
     6961 cttttcttca aactctgcaa catctatcgg taagtcaaac cagtatgatg ttgtttcttc
     7021 gccatttgtt cttcccgttt ccaccagtac ccttgtttcc tgcattgttc ctcacgtcct
     7081 ttctgttgtt tcatcacatc ttttaaggtc tggtatgaca taccaaacac atatttagaa
     7141 aaatctttca actgtttttc ttcatagtcg gcagggttca gccgtttcat ttcatgccac
     7201 accttacgct tgtaagaggg caggtcggaa atgtattcac agccgacttt tgcctgcata
     7261 tctttaaaaa tatcgttcat ttcatcactc caatctgaaa tttaggtaag aaaaaagcag
     7321 tcaatcctaa gactaactgc tttgtgtgta agtatgaatt tgtttaagtg taactgattt
     7381 accatgtgta ctcgtaaagt ttactgtatg tttgactttt aaataaatat atacaatcaa
     7441 tcatttgtaa cttgccgtct tgttcaaatt tacttattat cagattatct tcatagatat
     7501 atgctaagaa agtatcatac tctgcatagt atttttctct aaaaagattt gactctctaa
     7561 aatctttttc taaagcaatt cttatactgt ctaaagcata tttaaagtta taacaagtat
     7621 taaaactaaa cttgtatgga aaaaagaaaa ataagttttt cttgaaagta agcaatttca
     7681 cataggtttg tatatcaaat tcaatcgttc catactcata tttctctgta agacaagaat
     7741 gtaaactttc acaatcataa tcccgcaacg aagcaaataa cctagttaca gtcatttgtg
     7801 tatttggtct tctcggtgga cacttaccaa ttacaccatc acagaatttc tgtatttgac
     7861 cagtaagttc ttttgaagca tgaagtcttg ttgtgctttc aagtaattta aagtccattt
     7921 gataattagg tgagttacaa tcacattctc catgattttc agatttaggt ggtacatatt
     7981 ctgcaaaatt tgatttttct ctaaaatata ttgatttatt aactaattcc aacaagtatt
     8041 tttcataatt acaattttct tcatctttaa taaaattttt gtttatgaat actgcatgta
     8101 aaatatccac tctcatttca tctgaaatca taagcacttt tattcctttc aaaaaataat
     8161 ttatcgtacc cacattataa cattctcata ttacagcaac aataaaattc gttatgctcc
     8221 aataatctta ttgaacagct ctaacagtac atctttcaca ccgccagcgt tgaatactaa
     8281 gccgacagca ataagggcaa ctaccaagaa gccaataagt tttgaaaact cacgcttgaa
     8341 ccctaagtag ataccgataa ccacaatcgc cataagcact aagctctgtg catttgataa
     8401 aaaccattga tacaaatttt gtccgaaatt catttaattg tcctcgcttt cttctaattg
     8461 tttcatttca tagtcagctt ctttgctgat actcaaaata cacatcaaga ctacgccgat
     8521 acccattccc atagatacca gcaggaagtc ttttactaat tcccacattt tttatcactc
     8581 ctaactttcc accaaatctt ttgcaggggt cgtctgctgt ttgattatca tttcatgctt
     8641 ttcactcaag attgcctgct tttcaatcgt ttccatgtag tctgtaccgt ttcccttatc
     8701 aatctttttc agcattttca aggtcggtgc tacctgtctt tgtacccaac gcaatgtgcg
     8761 gtctaaggtg taaggttctg gttttgtcgt cagtttcaga ctttgccgat tatcgccaat
     8821 aaaccaagcc cagcggtcat tgagtttcca gtcatttttc cgcttgtctg gttcttcatc
     8881 aataaaccgc acatactggt tgatgataga aaaagcggtc tgctctgcgt catagtaggt
     8941 cagcaaatct cgtactgcat agtaagcacg ttcatttcta agccgtatct caaaacggtt
     9001 gataatgtcg gcttcttcca gaggtgtacc taatttgaca tactgctcat agtccttttc
     9061 atagatacag aaatacacat ctgatttcag cgaaccaaga taaagggtac tccccatata
     9121 ttctctgtcg tcctctctgt gcttgataag ctcgcccgac tggtaatact tatagcttct
     9181 ggactttccg atatattccc gtttcctgca tttctccgca agctctggaa tatccaaaat
     9241 gcccgtatgg tcgttgatag caaggtcgat acgcttcata atgcctccgt ctatgagtgc
     9301 gtccataaga aagtcatacc agcttctttg ctgtgccagc aggtaacttt caaactgcct
     9361 gcaaccccga ccctttaact ctaaaaggac acctttttct tcgtcagccg acgtatagat
     9421 aaagatgtcc cctaatgaat aatgctccgt ataactgtaa tgtccgtaat cttcatggag
     9481 catataattg atattcagtt ttaatatgtc tttgatgatg tgctgtatat ccagcgtggg
     9541 aaaacgaatt ttcacataat caaacagcat ggtaagcggt gcttctggat tgaaccttgc
     9601 cagttcttta tgcaaagttt ccagaagttc ttttgacggc ttcatttttc cgctttctat
     9661 cttgtttaga tattcccttg tgataccaga agctaccgcc agcctgcctt gtgagatacc
     9721 gtaagcaatc cgcttctccc gtaattcttt tatccattgt tcttcattca gaaccatacc
     9781 cccaatctgt caagtgtgaa gttttttaag gcgacttcac agccgatttt tgctaggaaa
     9841 ccatgccaga agccttatgt ttcctatgtt tttttgtcta ttgtctattt gcaaacgtac
     9901 ccctctgtta gataccgagg ggttttacct gctggcgtgg cttacgccac accagcaacg
     9961 gctagtccga gccgtcgcct ttcgcttcgc acgtcgccgt cccgtcctgc cttgcttgtg
    10021 caagaaaacc gattgtctgc aaaaaatcat gtcctttcgg gactaaaggc gtgtaaaatt
    10081 cacttatgac gcttgttccc acatcacaat agccacgccc tttgatacgc ttttggaaaa
    10141 actgcttttt cacatctgaa ccaaacagca taccgtaacc taactcgctg atacgcccca
    10201 atcccacacg taaattgaag ttatctctga taccgtccga gaaatatttt gcgtctggac
    10261 gctggcaggc aacgataagg aaataacctg cttgtcgtcc taacatgacg attttcttta
    10321 actggctaag taagcccaca ctttcttttg tccccagcat ttcaaaaaac gccacatatt
    10381 catcaaagat aagaaagcag ggtggcagtc ccagataggc gtaattttcg cccgtcttat
    10441 agttcgggtg tcgtttcatt tgctcgcttc gctgtaccat gccctcataa aaagcattga
    10501 cacagtctat catttcttct ttggtgtgat acacgtttcc catgactgtt cccaagtctg
    10561 ctaagtccgc atttttcggg tctaagatgt aaaggacagc gttggtatgc agtagggctt
    10621 caatgagcgt cagaagaaaa tatgttttac cgccaccagt cccaccagca atcagagcgt
    10681 gagggagtgc gtcatattcc cagacaagat ttttcatcag tctaagacag ccgttctctg
    10741 cccgtacttc atcaatggta atgcggttcg ctatcatatc ataaagcagg gtatattcga
    10801 tatagccgtc atgcagggtc ttgtcggtca gctcacaata caagccactt tccaatttat
    10861 cctctaaccg taaaagctgg tcttgatatt tccccagcgt aatttcacag cggatatgaa
    10921 gcagtccctt ttccatttga taataaatct ttggaaacca gacgattttt tcccttgatc
    10981 tgctttgcag gtcagtaaaa aaaacgctgt cttgtacggt atcagcttca taccacttgt
    11041 tttccagtat cattcgtgcc agcttttgac ggtgcagtag ctttttgaaa ctgtcgtaac
    11101 agaagcggta atacagaaaa gcgaccaatg cacaaacacc agtcgctatc agtatggtta
    11161 tgaagttgta aagggaaagc gtaaagccat tgtctaataa gctgaaatgt tcccagtcgg
    11221 tacgcatgag ctgttttgta tttaatagca ggataaccgc tacgaacaca aacaggagtg
    11281 tccccattga aaagcgatag acaaggttct tatcgctggc tctgatacgg tgtcctttgt
    11341 ttaaaatcat tctcataaat caatcactcc tttctgggta tgaaaaaagc agtcaatcgt
    11401 atagactaac tgctttgtgt gcatggatat gaagttgtca aactggaatt taacgttcac
    11461 tcatatattg ttttatttca tcagaagtca attttcgtac ttctgtatgt ggatattctt
    11521 tatactctcc aacattaaac acaggtgtca tttgtttgca tttcttgtct ttattctttt
    11581 tcaagaatag atgtaaatcc tcattcccgg cgaatatttt ataagatact ctgcctttac
    11641 tgttctctat ctcataaata ccacatgaac ttttcgttgt ataatcggct gttcgtaaat
    11701 aaagttttgc aatttccaat gacttaaaag gaaaattcca tctttgtaat cctctgtttg
    11761 ggtcatgttc aatacaaatg caacgtccac aagttccaca aatatattga gtgtggtttt
    11821 ctaagcagtc caagggattt agtaatggtg ttattctatt ttcatcagag taacattctt
    11881 cacacatttt tcttttcgct ccttatcaaa ttctaatttg tcgctttctt catacccata
    11941 ttataacagt tccgtatatc ctctacaacg aaaatctatt cagcaagtct attttttcgg
    12001 ttgtccctgc ggtgtatggt tctgtgaagt gcctgtgttc tgattgcctt tattcttcag
    12061 tacaatatct tctgccttta cataccagtc cacatctgca cccgtatagg tttttcgtga
    12121 taccgtatcg gctacgggat tgacaagctc cacgactgca ttgtaaggaa attcccttaa
    12181 cggtacttct ggcggtacgg acacggggat aattccacca tgcagactgc acttcaaatc
    12241 atagatacgc tttttaagct gggtactcgg tgtcccgtcc tcattttgca agaacacatc
    12301 acgcaccgct gtaaatttta attctccaaa tgttttctct ttgtcaatga caaatccatt
    12361 tgataatctc ataaattctt cactccttta cttttcttca actgctacaa tatcatcagc
    12421 tactaataca taatcggtat gtcccacatc cccgattgcg taccctctgc cgtataactt
    12481 cggattgaca agttttactt tctgttcata cttgaaatgc ttttcgccag cctgcacggg
    12541 aatttccacc acaacatttt cgcctttctg tacgtcagaa taaaggttat agcttcgtct
    12601 ggctatgacc ttgcggttgt ttttatccct ttcaaagata ggctcgcttt cgcctgcaaa
    12661 ttcaagagtt ccaaaagact gtgccatatc tggcacgaca tatttcattt ccatattgtt
    12721 ttacctcgtt tctttctata tttgataagt ttatgattgt aattttttat tctggcttgt
    12781 ctgccgtttg acagtttcgg cggagaacgt gatttttctt tcatcatacc gttaccgcct
    12841 tatgattttt tcattgtacg gcgtttgtaa agatatgttc cagccagtcc accagcagat
    12901 gtaagcaaca tcacaagaaa tgccatgaca tttgtactgt cgcccgtttt aggactgtcg
    12961 ctaggtctgt ttggcttttc tggtgtctgc ggtgtttcga gtttctctgg gacttctggt
    13021 ttttcattaa aagtaatcgt ctgtccctcg tcctcaatat ccttatgctc ggtaactttc
    13081 ttcggctcgt ctggattgct taaatcatac aattcttcaa aagttacaag ctgtttgccg
    13141 tcaagggaag tagcgtcaaa ggtaaaggca acttccgctt tcatagtttc gctgtcagcg
    13201 gtaaaggtgt aatcactttc cacacgtttc ccattgataa gaagttctgc attttcttct
    13261 ttcaacatct gccagcctac aagtttgtac tgtgtaccga cttccaagcc ctctaaggtt
    13321 acggtatcaa tgattgtaac gtcttttcca gcttcaatct ctttgttgcc gtctttgtcg
    13381 gtagcagtcg tatgtatctt gatgatacgt tctgtaatca atacagtttg tccgtcgtcc
    13441 tcaatgtctt tgtgttccgc aactttcacg ggttcgtctg gattgcttaa atcgtataat
    13501 tcctcgaatg ttaccagatt tttaccgcca agctcggacg cattgaatgt ataagaaatt
    13561 tccactttca tttcttcatc atcagcgata aaagtatagt cattttccac acgttttccg
    13621 tcaatgataa gctctgcatt ttcttccttt aacatctgcc agcctttgag ctgatatttt
    13681 gtaccttttg taagcccgtc taatttgaca gtatcgacga ttgttacctc ttttcctgca
    13741 aggatagtct tttcgctgtc tttgctggtc gctgtggtat ggatagagat ttctttttcg
    13801 tattcatcag tcaaagtccc taaatcaatc acaaggttat ttcttgatac cacgatttca
    13861 aaaggtggga taagttcaaa gcctttgtta ctatcgcaac gcatttcttc aatgatgtaa
    13921 gtatcataag gcaatgcacc tttgctgtcg tctgcttcag aagttccaaa ccacacaccg
    13981 tcctcgctgg tcttgcctgc gttagtattg tgcttatgag aagtccattc agcagaagtg
    14041 gagaattgcc cgttatcatc agtaactaca acatgacttt ctcccgtcgt cttgcttgtg
    14101 atcctaaagg gaacatcagc aagacgcttg tgtgtgcctg caccgatttt cactccctca
    14161 atatccccac gcttaatctg attgtagata gagtgggctt cgtcggttaa gtccacgatt
    14221 tttccattct ctgtgattgc aaagtcaatc ggcttcgcac cgtctgtcaa gtaaccgtcg
    14281 ggagcttcac tctcaacgat acggaatttc ccataaggta aaaggtcagc agaagtagaa
    14341 gccacaccct caatatctgt acgaattgtc tttaccactt catttttctt atatagcttg
    14401 ccctcaacca aaacagaatt gtcatttaag gaaatgacgt caaaagcagt atctttcaaa
    14461 gtggcacttc cttgtggttt ggtatcgctc gtttctaaat ctcttttctg aattttcaca
    14521 ccgccacgga taaccttgtc tgatacggaa aactggttac ttcccgtcaa tacagcaagg
    14581 tcgccgtcct ctgtaatttg tgtaaggtat aagcctttta tctgttcaga cttatcgcca
    14641 gcctgcatat atgcaccgtc cagcaagtag ccgtttggag cttttgtttc ctcaacagat
    14701 agtgttccta aaggaagaat cgctttgccg tcctgcatat agaagctgtc gccagatacc
    14761 ttgtaagcgt ccgctaattt tgtgatgtag tgagttgtcc cgtcactgtc tgtttcagcg
    14821 attgtctttg taacccatgt acgagtagct tcggcaggga gattgtcttt gttatagaag
    14881 ccagcataat acttccatgt aaactctgca ccctctaaag aagcgtttcc ctgcggattg
    14941 tctttctgcg tttccatatc aatcttgaaa agctcaatca aggtgtccgt tacttttggt
    15001 gtatctgata cgttcaaggt cgctgtcttt ccagcttcaa cttttaagga atacacggtt
    15061 gtatcaactt tatatcctgc tggtgcggat aattccctaa tatagactgt acctgctttt
    15121 acctctacaa catctgtatt tccgttttta tcagtcgtaa gggtggcaag ctgtttcgtg
    15181 cagtccttat cagaaaagac accataggtc gcaccagcga ttgagtaatt cccgttaccg
    15241 tctgtaatgc tggtattact ggaagtcttt tgcagttttg catttccgac atttaacttc
    15301 gcccagaatt gtcccaattc ctgcccctcg ccagagtaga tataaccgcc acattcatag
    15361 cgtcctttat tttctttcac aaaggcttta gcaccagcga aaacttcgtc ctgagttgct
    15421 tttggtattt catcataaga agctcgcacg ttgtcgcatt gccagccaag atgtacgctc
    15481 aatctctgcc agacaacaca ttgttccaac agatagactt gcttgtagtt caattccttg
    15541 tgtgcttcgc catactgttt gacatactct aaggataacg ccacatctga aatctggtcg
    15601 gcactcatac gggtgcttgc gtcagctctg gtcttgtagc cattcttaaa atctgtattg
    15661 atgtctatac agtaagcgtc ctcgccctca acagtcaaat gtccctcgtt aaaggtagaa
    15721 ccaatcgaac cgtcattcat tactttttct acgataccga cacgttcctc tgattccgtc
    15781 cagtattgct tgctttctgc atggacggcg gtactcggta aagcagtaac gacagtcgca
    15841 aaggcaagta ctcccgtaca caatcgtttc aatatctttt tcataattct aaatgctcct
    15901 ttcattttgg gtaaaaaaat agacgctcaa tattgaacgt cttacattga aaagggatac
    15961 tttttacaag tacccctcaa atctaatatt taattgtttg tgttcccaca ctctatatca
    16021 acaactactg aaacattgat atagtttttt atctctttat ttctgatttt atcgccctcg
    16081 ctcgtaaaga ctatatgctt aaaaaagtgc ctaaaattaa ggatttctac atagttcgtc
    16141 tttgtagcaa cacacaagtc tctatatgtg tagaatgagg gaacatatct acaggttgtg
    16201 cctcaacaca tttaaatccg tgaacattta gataatctaa atcccttgct aaagtagaag
    16261 gattacaaga aacatagaca attctttccg gattcattga aacaatagtc tctaatacct
    16321 tttcgtcaca tccctttcta ggtgggtcta caacgactac attggcagtc ttaccctctc
    16381 tatacatctt aggtacaatt tcctctgcct ttccaacata aaattcagca ttatgtatat
    16441 tgtttaattt agcatttatc ttagcatctt ttatggcatc atctataatt tctattccat
    16501 aaaccttttt agccttttgt gccaaaaata aagatattgt acctatccca cagtaaatat
    16561 caaaaacagt atcagtatca tttaaatttg catattcaag agccttatca tacaatactt
    16621 tagtttggac agggtttact tggaaaaatg ataatggtga aatctcaaac actaagtctc
    16681 ctatataatc gtttattttt ccatctccat atataactat attttctctt cctagaatag
    16741 cattcgtatc tttttgattt acatttacaa ccaaagtctt aaatccaggt atattttcct
    16801 taagcactga agctaattct ttaagatatg gaagtttgtt tccatttgct actaatacaa
    16861 ccattacttc tttagttgta aaacctattt ttgttactaa atgcctaatc aaccccgtgt
    16921 gagtcttttc atcatatatg cttaccttat aggcccttat atatgttttt attattttaa
    16981 caattttatc atttatctca tgttgtatta cgcatttttc tgtcgatatt acatcatgag
    17041 tcttcttttt atagaagcca ataattggaa taccattatt cttttgtatt ggaaattgtg
    17101 ctttatttct atatctatat ggattatcca ttcctatagt gtcatgaact tctacatcct
    17161 ctaacttacc tattctcgaa agtacttgtt taacttcatt tgttttcatt tcaagttgtt
    17221 tattataatc taagtcttgt atctgacacc ctccacaatc cctaagattg tcactacaca
    17281 ctcttttcac cctgaatgga gacttttcta ttatctcaac aatatctccc acagcataat
    17341 ttttctttac tttattaatc ctaactttaa ctttatcatg tattaatccg ccctctacaa
    17401 aaactgtaat accattgtac tttcctactc ctactccgcc ttgaccaatg tctactatat
    17461 ctacaatata catctcgttt ttcgaaagca taaatcaccc tccaatcaat ctattatatt
    17521 taaatcttta caaacttttt tctttaaatt tatattaaaa tattgctatt aattaaacta
    17581 tattttttac taagctacaa tattttattt taccaaaaaa aaaggagctt ataaagcccc
    17641 ttagtaaata aatttattat ttttcttata aaactctagt tgaacctcta tataccacgc
    17701 ctcttgatgc atccacagtt acaacttctc catcttttac tagatttgtt atatcagctg
    17761 cagacacaat aactggtata tctaaattta tacctacaat agctgtatgt gatgtcattc
    17821 caccattctc agttactaca gctcctgctt tttctatata attgttcatt tcagcatcag
    17881 tagcagtaga aacaagtatg tcgccttcat taaagtctac acaagcattt ccattcttta
    17941 ttatacgaac tttaccttct actgtttgag tacctacacc tataccttgc acgatttctt
    18001 cgcttattac atgaaccttt attaaattag tagttccact tactcctact ggtactccag
    18061 cagttataac tactaattcc ccattatcta tataatcagc ttgtctagct gcctcaattg
    18121 ccttttctat aacttcatct gtatttcctg caacagaaga cttaactgga tatactcccc
    18181 atgtaagagc taatttattc atagtctttt cattatttgt agtagctatt attggagatt
    18241 ttggtctgaa tttagatacc attctagcag tatatcctga agaagtagat gttattatag
    18301 ctgatgcatt taaatctact gctgttgtac aagttgcatg gcttattgca tctgttacag
    18361 taacattgtt agtaccattt tcctttagta atctatcata atctaaagtc tcttctgttc
    18421 tttttgctat tgtagccatc atttttactg cctctacagg atattttcca gcagcagttt
    18481 ctccagataa cattatcgca tctgttccat catatatagc attggcaacg tctgtaactt
    18541 ctgctcttgt tggtcttgga tttcttatca tagaatcaag catttgagtg gcagtaacaa
    18601 ctggctttgc taattcatta cattttttta tcatcatttt ttgaactatt ggcatctctt
    18661 cagttggtat ctcaacacct aagtcacctc tagccaccat tataccatca gaaactttta
    18721 atatttcatc taggttctct acaccttctt ggttttctat tttagatatt atttgtatat
    18781 cagttgcatt attgttctct aatacttctc ttattgctag tacatcagat gcctttctta
    18841 caaaagaagc agctatataa tctattcctt gacttattcc aaactctata tcacttatat
    18901 ctttaggagt tattgctggt aaatttatct taacaccagg tacatttact cctttatgat
    18961 ttttaactat tcctgaattt tcaacaacac aaactatatc ttctccattt atttctttta
    19021 ctcttaagcc aactaaacca tcatctatta gtatagaatc tcctgattta acgtcttcag
    19081 caagaccttt atagcttact gtacacattt ctttagtccc cataatatct ttcatagtta
    19141 tagtgaactt ttgcccttct tctaaaaata cctctgggtc ttcaaagtta cctgttctaa
    19201 tttctggacc tttagtatct aataatatag caactggctt atttaatttt tctctaactt
    19261 tctttgccat atcaattctt tctttatgtt cttcatgcga accgtgagaa aagttaaatc
    19321 tacacacatt tagaccattt tgcataagtt gagttaaaac ttcctcactt tgtgatgctg
    19381 gacctagtgt acaaacaatt tttgtcttct ttacattatt taacatattt gctcctcctg
    19441 agatattatt ccacagattt atttttaata ctaaaactta tatagataat atttttgcca
    19501 tctcataagc ttctttatca aatactttct tttgagctaa agcttcactt atttctaaat
    19561 caattatttt gttttctttt attcctacaa ctctagctga ttttccatct aataataatt
    19621 ctactgctct tactccaagt ctacttgcta gtattctatc agatacagtt gggcttcctc
    19681 ctctttggac atgccccaat acagtgactc ttaaatctgc tcctgactct tttttaagtt
    19741 ccttttctaa atcactagca cttccgactc cttctgctaa aacgattata ctatgtctct
    19801 tacctctttt ttgagtagtc tttaatctta gagcaacttc atctactgta atttctactt
    19861 ctggtactat tatagtttcc gctcctcctg caagacctgc atataaagct aaatctccac
    19921 agtgtcttcc cataacttct actatgttaa ctctttcatg agatgatgat gtatctctta
    19981 tcttacctat agcatctata atagtattca ttgcagtatc aaatcctata gtataatctg
    20041 tatatgctaa atcgttatct atagttcctg gtatgcctat agctgggaat cctaagtcac
    20101 taagtttttg ggctcctgca aatgaaccat ctccaccaat tacaactaag caatcaattt
    20161 tatacttttt aagaattttt actgctttta atctaccttc ttctgtttta aattcttcac
    20221 atctggaaga ttttaatata gttcctcccc tgtgtataat atctccaaca gaagataagt
    20281 tcatttcagt taaatcttct tctagtaatc ccttatatcc tctattgatt ccatatactt
    20341 tgcatccata atatattgct gatcttacaa ccgctctaat cgcagcattc attcctggag
    20401 catctcctcc acttgttagt aatcctatag ttttcatttt ttacctcctt ggattttatg
    20461 tcaatttttt attataatca ttgttattgt attctaatgg actttttttg tccctatatc
    20521 actttttaat cccatcaaga ctaaaaaaat taaatgtcct taaatgtcca ttttgtgata
    20581 ttatatcaca aaacggacaa tatttttcat attattataa tatatttaga tttttatata
    20641 aaaaatcaag cttttacttt tatgttatct ttaggaagta actcttctaa gttatttata
    20701 aattcatcac atatacaagt caatgccccg gtcattttat atgctttacg tttaccattt
    20761 atattttcat ccatagggta taagtatata ctgtcattac ctggatattg tttggctatt
    20821 tctacaatat tattagttgt atcagtatca gacatactat ctattttcaa atacaatttt
    20881 ttaccacttt ctggcttaat attttctaca tttctagttc ttcctgaatt gtagtttcta
    20941 tctgcaaagt cagtatcatc atttatatct tttatctctc tagctataag ttttgcattt
    21001 tcgccttctt ttatacttaa tgttcctttt acatatatta tattatcttc attcaaaatg
    21061 acattatact tttgtaataa ttgaggaaaa actattattt ctatagcacc atacaaatct
    21121 tctaattcaa taaatgccat aatttcattt cgttttgttg ttttaatagt tttagttaca
    21181 atcattccac ccattataac atctgtttca ttcatttgta ggtatgactc ttcttcctct
    21241 tttaatgaat ttaattttcc attatccata gatgtttttt cttgtagttc tttttcaaat
    21301 tgtgataaag gatgtccact tacatacatt cccaagactt ctttttcaag gttaagtctc
    21361 tctctttctt cgtactcttg ccttttaggc aaactgtata tttgttgaaa atcactatct
    21421 tcctcactat ctccaaaagc atcaaataaa gaaatttgac cttgaacatt tttctttcta
    21481 tccatagata cactttctaa taaactttca taaccagcca tcagactagc tctattttca
    21541 cttatattat caaaagctcc acattttatt agactttcta ttactctctt atttgtatct
    21601 ttttggtcta atcttttgac taaatctact aaatctacaa ataatccatt agattctctt
    21661 tcagcgatta tattgtttat tatatttacg ccaacatttt ttacggctgc tagaccaaat
    21721 cttatattgt cgccttcaac tgaaaacttt gaaaaacttt tgtttatatc tggaggtaat
    21781 acatctattt ctagtgcttt acattctctt atatactcaa ctaccttatc agtatttccc
    21841 attacactgg ttatcaatgc tgccataaat tcaactggat aatatgtttt tagataagct
    21901 gtctcatatg ctaaaacccc atatgcagct gcatgtgatt tattaaatgc atatctagcg
    21961 aagtctatca tatcatcaaa tattttatta gctatttcct cttctacacc atttctgata
    22021 caccctgcaa tttctatatt accatcttca tcaaatttac catgaataaa atattgtctt
    22081 tcttcttcca tcacgtccat ctttttctta ctcatggctc ttctaactag gtcacttcgt
    22141 ccgtaactat aaccacctaa atctctaacg acctgcataa cctgctcttg atataccaaa
    22201 caaccatatg taacttccat tataggcttt aatttttcat gtagatatgt tacatctttt
    22261 gggttgcttt tattttttat atatgcaggt atagaatcca ttggacctgg tctaaatagg
    22321 gatattcccg ctacaatatc ttcaaaattg tctggtttta gctgtttcat aaaacttctc
    22381 atgccagcac tctccaattg aaacacccct agtgtatttc ctgatgaaag catttcatat
    22441 actttcgggt cgtcataatc catctttgaa aagtctattt tttctgtgta attttttata
    22501 actctatttt tttctattaa atcaagagta tctcttataa ctgtaagagt tcttagccca
    22561 agaaaatcca tctttaaaag cccaagttct tcaagtgttg tcatagtaaa ctgtgttgtg
    22621 attgcatctt gatgtttgta caaaggtaca tattcatcta cgggattttt tgatataaca
    22681 actcctgctg catgagtaga agcatgtctt agcattccct ctatctgttt tgatatattt
    22741 attatttctt ttgtttccgg gtcttggtca tacaattctt taagattagg atttgtatct
    22801 agggccttat ctattgtcat tcctaaagca aatggtattt ccttggctac cttgtcaact
    22861 ttattgtagc ctatatccaa tacccttcca acatctctta ttgcagcttt tgcacccatt
    22921 gttccaaaag ttattatctg ggctacatgg tcatcaccat attttctctt tacataatct
    22981 ataacttctt ctcttctctc ataacaaaaa tctatatcta tatctggcat ggatatacgt
    23041 tctggattta aaaaacgctc aaataataga gagtacttta ttgggtctat atcagttatt
    23101 ttaagtgtat atgctactat cgaaccagcc gcactacctc tacctggtcc tactataata
    23161 ttattttctt tagaaaaatt tataaaatcc caaacaatta aaaaatactc tacataaccc
    23221 attttttcaa ttacatctaa ttcataattt aatctctcta gtatctcatt actaggatta
    23281 tcatatctct cttttaatcc attaaaacac aactccctta aataagtttc tggtgtatat
    23341 ccttctggaa catcatactt aggaagatgt atagtattaa aatcaaactc cacattacac
    23401 atatcagcta ttttcacagt attgtcaata gcctctaatg catatggaaa taattcttcc
    23461 atttcttctc ttgattttaa ataaaactca tcactaccaa atctcattct atttgggtca
    23521 tttaatgttt ttcccatttg aatacacata agtacatcat gtattttcga atcttcttta
    23581 tttacatagt gaacatcatt agtagcaact agtggtatac cagtctcttt tgataatttt
    23641 actaaaccag agtttacttc tttttgctca ggtaaattgt gatcttgaat ctccaaaaag
    23701 aaattttctt ctccaaatat gtctctgtag tcaagagcaa attgttttgc tttttcataa
    23761 tttctattca ttaaagcacg tgctacatct cctgcaaggc atgcagaaag tgctataatc
    23821 cctttgttat gttttttaag ttcttccatg tcaactctag gcttatagta aaaaccttct
    23881 acgtatgaag ttgaaactat ttttattaaa tttttatatc cttccatatt cttagctaac
    23941 agaactaaat gtccttgata tttgtcatag tttgggtctt tatctctcaa tccacgtgca
    24001 gctgtataga cttcacaccc tataattggc tttattcctt cttttatagc tgttttgtag
    24061 aaatctataa caccaaacat acaaccatgg tcagttatag ctattgagct catgtttagt
    24121 tccttagccc tttttataag ttttttcacc ctcgaaaacc catcaagtaa actatactca
    24181 gtatgtacat gaagatgagc aaaatctttg ttcattaaat cacctcctgc tttataattt
    24241 taattttacc acaaatatat gtcctaaaac aaaaacagag acttttatct caaaatagga
    24301 attaacttca acatatacca ttatatgtta taaattaatg cttattctga gattgtcccc
    24361 tcaatttatt gctatgattt ttttttcaaa tctgttgcaa tttcttctat ttttctaagt
    24421 ctatgattga ctccagattt tcctattggt ggttctaaca tctctccaag ttctttgaga
    24481 gtcatatcct catactcgat tctaatctta gctatctctt gtaaattttc tggcaaactg
    24541 ctaattccta tagtctcttg tatgagctta atattttcta cttgtcttac tgctgcattt
    24601 actgttttag aaaggttagc tgtctcacaa tttactatcc tgtttacatt atttctcatt
    24661 tcttttacta ttttagtatt ttgaagactt aaaagagcgt ggtgtcctcc tataatactc
    24721 aacaaatctg atatttgttc actctctttg atataaacaa cgtgattatt ttttctagcc
    24781 acaattttac tattaaaacc taaagagttt ataagttctt ttagagaatc tgcaaactcc
    24841 tcattattag ttacaaattc catatgatag ttcttttctg ggtcgcttat agaacctcca
    24901 ccaagaaaag ctcctcttat gaaagctctc ttacatcctt catgcttaat taagttctct
    24961 gaaattttgt ttatagtaaa aaatccttct ttatcttcaa gtatacctaa cttttttaat
    25021 aacatttctg aacccatttc gcttgttacc attaatacat aactgttatt tctctttagc
    25081 atttgattct tatttactga gattgtagta ttgatattaa aattcttttt taataattta
    25141 aatactttac gggctattga atttaactct gttgttatct ttagatttag cttcttatat
    25201 ccaacaattt gtatacttcc ccctgattta actagagctg acaattcagc tatattacaa
    25261 cattcattcc cagatataac tcttgctagt tcatttttgg tttcagttga aaaagacata
    25321 tctatcacct attaactaat ttttctttaa cctttttttt atttcttgta aggcaacctt
    25381 tattatctcc ttttcatcat catacctagc taagtctact actctatcta ggtggataaa
    25441 tttagcatca ataaagcctt cctctttttg tggaatagta tccatattct ttgcttgcat
    25501 caaataccaa aaaactgttt tatgaactgc tctattttca tcccagtttt ctttgaatgt
    25561 atagtgtatt tcacctaaat atttcaatat atctgctttt actccagttt cttcacttac
    25621 ttcacgtaat gctgcctctt ggttattttc cccttcttct acttttccct tgggtaacac
    25681 ccaatctcca ttgaacttcc ttagtaaaag aatcgtattg ccaaacagga ctacaccacc
    25741 agcactaacc tcttctctca tcgatgttca ctccttaaaa ctaacataat gataaggatt
    25801 tctacacaat tttttaaaat ccttttttac taacttttgt tgtaattatg atttagggct
    25861 aactcaataa ctatgttaga tatatacttt gcatcatgtc ttatataatt attttttatt
    25921 tcaattaaat tcttctctac agtttttata cccatctctt ttaatttatc tttttgcttt
    25981 ttatctaaca agacttgctc tgctccatct tgtttatata agtcaagcat ttcttctggc
    26041 aatatctcat tatttgcgat tacataatct attaaattct catcagtgtg ttttactata
    26101 gcattaacat gctctaatac attgtaccct tctgtctcac ctggttgagt cattatattt
    26161 gctatatata cttttggagc tacagacgat ttaatagcgt ctacaatccc ctctacaagt
    26221 aaattgggta ttatacttgt atataaactt ccaggaccca taattataat gtcagcatca
    26281 taaatagatg caataacatc ctttaatggt tttacatctt ttggatttaa aaaaatttta
    26341 tcaatagagc ttttttgaat ttttgactct tcaggtatac tagattctcc atttattata
    26401 ttgccatttt ctaatttggc aactaaattt acatcttcca aagttactgg taaaactctt
    26461 cctgttatag caaaaatttc acttaattta tatacagctt tttcaaagtt tccatacaac
    26521 ccattcatag cagccaaaaa caaatttcca aaactctgac cttttaaaag cccttctgta
    26581 aacctatatt gcataacttc attcattgtt ggctctatat ttgcaagtgc taataaacag
    26641 tttcttatgt ctcctggtgg gagcattcct aagtcttctc tcaatacacc tgaacctcca
    26701 ccatcatcag caacagttac aattgcagtt atattttgcg tagtgtgttt aagacctctt
    26761 aaaaataccg attgaccagt acctccacct atcacaacca catttggttc tctattttca
    26821 acaatagacc tttgaaatag ttttactttt tcattttttg cttttttata tataaataac
    26881 gatacaatta atgcaatcca acctaaaatt cctacaataa taagtaattt agccatgttt
    26941 gcctccatta tcgcaacata gagtctctat gtttctttac aactctatat cccttattag
    27001 ataaatcctc cgctataaga tttgttatag taactgacct atgtcttcca cctgtgcatc
    27061 caacaccaat tactagatgt tgttttcctt cctctatata ttgaggtact aaaaaatgaa
    27121 tcatatctaa aagtttcaca taaaattctt cactgatttt agaattcata acataatctc
    27181 taacttcttt atcatcacca gttttagctc ttaactcttc aacataatag ggatttggaa
    27241 gaaatcttac atcaaatact aaatctgcat cagcaagtat accatgttta aatccaaaag
    27301 atactacaga aatagttagg tttggattat cttcacctga agaatatatt ttctttattt
    27361 cttccttaag gtctttaggt ttcatgttag ttgtatcaat tatacatgta gataactcct
    27421 ttaaaggttc cattatcttt ctttccattt ttataccttc tggaatctgc atatcttttg
    27481 ccaatggatg atttcttctt gtcattttat atctctttaa caatacatca tcattacaat
    27541 ccaaatatac catttcatat tcataatttt ctttttctaa ataatttaag ctttcatgaa
    27601 gcgcttcaaa aaactttctc cctcttatat ctatccctag agcaacttta tcaatacttg
    27661 aatttggttg ataacataat tctgcaaatt tagttatcaa agcaggaggt agattatcta
    27721 cacaataaaa tcccatatcc tctaaagctc tcattgtctc actttttcca gatcctgaaa
    27781 gacctgtaac tataacaaat ttcattgcta actcctccct tttatcttat gtttattata
    27841 ttataatgtt ttctactaac gatatatcca gattagagtc agatatagtc ttcatagcta
    27901 gttcaaaatc accaactctt tctggacaat gtgcatctga gcctattata aatttatttt
    27961 ctagcccttt tattttattt aattgattta cattcaaaaa agaatggcta ctatttatct
    28021 caagtctagt atttgtcttt tttgctgtct ttgctacttc ttctatatac acatcgcctt
    28081 tatctcctgg atgagtaatt ataaaaatat cattattact tatcgcattt attacagctt
    28141 ttgtattata ttcttttgct ttttcagttt taaaaatata gttattacaa tgatttaata
    28201 agcttcttaa tgatgttgga gtagaaccaa aatgatatcc agccataata taatcaaaat
    28261 ctctaataaa tttatcatcc atatctatat ttcctttatc atctaatatg ttacactcca
    28321 tacctaaaag tatatttata tcagtgtact tatcatttaa ataatctact tcttctctca
    28381 ttttgagaat atcttttttc tttacaccat atgcaatatg tttataacta tggtcagata
    28441 ttcctattgt ttttattcct ttagaaatgg ctacctttac attatcctca atagtccctt
    28501 tgccatgact atataatgta tgtgtatgat aatcagccag tattttcatt tgttaatcct
    28561 ctccaagtat tttgacttct tcttccaaca taaccccaaa cttggcatat acagcacttt
    28621 ttataacata tattaaatcc aaaatatctt tagcacttgc ttctccttga ttgactacaa
    28681 atccacaatg cttctcagat acttgtgctc ctcttaaagt aagacctctc aatccagttt
    28741 cttctattaa tttacctgca aaatatcctg ttggtctctt aaatgtactt cctgcactag
    28801 caaaatttaa tggctgttta gttattcttc tcgtcgctaa atcatccatt aaacttttta
    28861 tttcatcata atttccttct tgtaattcca attctgctga taaagctata tatccgtttt
    28921 tcgatattat actttttcta tacccaaatt ccatctgttc atttgataat tcaaatatat
    28981 tgccttccat atccatcagt cttactgact tcactatgtc cttcatttca ccaccatatg
    29041 ctccagcatt catggctaat gctcctccca atgtgccagg tattcctgat gcaaactcaa
    29101 acccttttaa ttcttgcttt tggagagctt tccctaaaac tgataataaa gctcctgact
    29161 gagcagtcat cctagtatca tttatctcat aagaattgaa attatcagct agttctatta
    29221 ccactcctct tattccacca tctttaatta aaatgtttga accatttcca attattaaat
    29281 acggaataga ttcttctttt accaacttaa gtacattttt taattgttct tcagttctag
    29341 gtctaactaa tatatctgct ggtcctccta ccctaaatga aatatgtttt ttcataggtt
    29401 catcgacttt tatatcctct ttacttaata tatttaataa atttatatat atatcttgat
    29461 tattcattgt atttcctccc aaattatact gaactttagc tgtcattatt atttaaatta
    29521 cattagtata tagtattaac tatattcaat aatatatgat tttgcttatc atatggattt
    29581 tatattttaa gcttcttaat tacaaactat attcaataaa tatataataa agtaaaaaaa
    29641 aaaaaataac aacatataaa agaatataaa taatttaaaa aaagcatcaa tatcactgat
    29701 atcaatgctt taaaaggcaa tttaattcgt attatttatc taactatttt tgacatagca
    29761 aagataataa atccctccaa ctactatgga acctccaacg atatttccaa gcgttacaaa
    29821 taccaaatta gttataaatt gaaccatagt tatatttgct cctaatgcca ttcccattgg
    29881 tataaaaaac atgtttgcaa cactatgttg gaatccacat aatacaaata gcattattac
    29941 tgtccaacat gaagccaatt ttgacattat atcttgagat gctgtagcca tccatacagc
    30001 taaaacgact agaatattac aaagtattgc ttttaaaaac attgatgcta tagttgaatt
    30061 aaccttagct tctgccacag ctatagcttt tgtagctgca tctcctgtca atgtagaaga
    30121 ataaaacaca agtgcaacta gcaaaattga acctactaaa tttgcaaaat aaaccagtcc
    30181 ccagtttctt aacatttttc ctaatgttat ttttttgtcc ataagtgcaa tagccattaa
    30241 ggtgtttcca gtgaataatt ctgctcctgc cactactaca agcataagac ctactggaaa
    30301 gaccatagca cttaaaaagc tagctacccc tgggtctatc ttgccaaaag tttgactaac
    30361 tatcatactc cccaatgccc caaaacctat aaatgctcct ccaaataaac ctaatagaat
    30421 tgcgttttgt gtcgaaatat ttgctttttt aatgcctgca cttatagttg cttgtgcagt
    30481 ttccccaggt gtcaagaatc ctttactcat aaatccttcc cccttaaaaa ttatactttc
    30541 ccttgtcttt ttatggcaat aattatataa taacaatatg atatataata aattttatta
    30601 acataaaaaa tatactatat acttatatat acttgtctca tataattata ctatttatta
    30661 tataacaact ttgttaattt cgaaataatt tatattgaat ttgaaaaatt taccttttga
    30721 ttatttattt tactttttaa ttattgaggt attatatata atagatatat gtttgtgtta
    30781 ttatatataa ttacttattt taataaatat ttgaaggagg gaattttatg tttgatatat
    30841 catttttcga aatgtcaata tatgaaatag cagttactac aatatatata attaatttca
    30901 tggttatact aaaccttatc tttagagaaa aaagaaacat aaatacaact ttaacatggc
    30961 ttcttatatt agtattgatt cctgctcttg gattcatact ttatatagct tttggcagaa
    31021 atatttcaaa aaacaatatg tttagattga aagaaaagga tgataagata atcaaaagta
    31081 atatactaga tactcaggtt aaactccaat ctacatctga aattgattca gatatacatc
    31141 aacataagga tatgatttat gcattggcta attcaaataa tgctcattat accaataata
    31201 acgatgtgtg gatttatgct gaatctagtc aattttttaa cagtcttctt gaagaactta
    31261 aaaaagctaa aaaatatata aacatacaat tttacatttt caaagatgac aaaatcggaa
    31321 ctgaaataat agacattctt atagacaaag ctaaagaagg tgttgaagta agacttttat
    31381 ttgatgcagt tggtggtaga acactaaaaa atagtacact ttccagactt aaagaaagtg
    31441 gtgttaaagt tggaagcttc ttcccatcat tcttgaaaat tgtaaacttt aacttaaact
    31501 atagaaatca ccgaaaaatt gttgtcattg atggtaaggt aggatttgta ggtggatata
    31561 atgttggtga tgaatattta ggtagaaatc caaaatttgg tctatggaga gatacacata
    31621 ctaagcttac tggtgattgc gtaattgacc taaatatgag attcatcctt gattggagat
    31681 atacaactaa agaagactta gatttagaaa agtactttat agaagaagat acaaattcta
    31741 atcatccttc tgaaaatatt gggattcaga tagtatcaag tggtccagat atatcagaac
    31801 ttgatgagat taaatatggt taccttaaaa tgattcagaa agctagaaag tatatataca
    31861 tacagagtcc ttatttaata cttgactcaa cttttataga tactttaaaa atagcttgtt
    31921 tatctggtgt tgatgtgaga gttatgattc cttctaaacc tgaccatccc tttgtatact
    31981 gggcatcata ttcatatgct ggagaacttt taaagtttgg agctaaaatc tatacttatg
    32041 gtcaaaatgc atttttacat gctaagacaa tagttataga tgactctata tgctctatag
    32101 gaacagctaa tatggatatc agaagttttg agcttaattt cgaagttaat gcatttatgt
    32161 actcttcaaa aaaagcattg gaacaaagaa ttatatttga aaatgatatt ttaaattcta
    32221 aggaaataac attagatata tataattcta ggtctactta tataaaaatc aaggaatcaa
    32281 tttcaagact attatcagca gttttataaa tcttttattt tataaaaatt ttacattttg
    32341 gtttattttt taaagttttt tatatgtatt attttttttt tattgaattt agctcatttt
    32401 ttttattttt tttaactatt ctgaattatt ttatattata atagactagt atagttttta
    32461 tttttaatag gtaacttttt tgcattgttt ttataaataa agaaattgtt gtatattatt
    32521 ctttctttat tgttttggtt ttgttcaccc acataatgtt ggggtcaact catggttaat
    32581 tattttatga aaaataataa ctttaaaaag aaaaggggga tttgttatgt gcgattttat
    32641 ttcacacaac aagcaattat ttgacgaact agatatattt attgactctc ttgcaacaaa
    32701 ggaaggtgcg ttaatacaag ttctacatga agcccaaggt atatttggat atcttccaaa
    32761 agaagttcaa cttcatgttg ctagaaaact tggagtagct cctgcaaagg tatatggggt
    32821 tgttacattc tattcttatt ttactacaga acctgtaggt aagtataaaa tcagtgtctg
    32881 tctaggaact gtatgttttg taaaaggtgc tgacaaaatc ttaagtgctt ttgaaaaaca
    32941 gcttggcata aaagttggag aaactactag tgattttaaa ttttctttag aaggtttaag
    33001 atgtttgggg gcatgtggtt tagctccagt tgttactgta aatggtaaag tttatggtaa
    33061 agtgaaacct gaccaagtaa gcgaaatatt agatacttat agagaattag aattgaattg
    33121 ttaagagggg tgaataaata atgcgtaaag taaattcatt tgatgaatta aaagttttag
    33181 ctgatgagct gaaacctaat cttagcttaa gaaaaaatta ttctaaggaa aatgtattaa
    33241 gaagagaatt acttgtttgt tgtgatactg gatgtacatc ttcaaacagt ctagaaatag
    33301 ttagtgaatt agaaaatgaa ataaagaaat caggaatcca agataaagtt agtgttcgtt
    33361 tgacaggttg ttttggtttc tgtgctcaag gtcctattgt aaaagtttat cctgataacg
    33421 ttttctacgt aaaagttgag ccatctgatg ctgaaaagat agttcaaagc cacttaatta
    33481 gaaatacagt tgtcgaggaa ttattatatg aggaagaatc tttaagtaaa aaagttgaag
    33541 aacaagaaga aatgtctttc tacaaaaaac agttacgtat agctttaaaa aattgtggtc
    33601 ttatagaccc agatagtata gaagaatata ttgccaatga tggttattta gctctaggca
    33661 aatgtttaac ttctcttact cctcaagaag ttattgcaga ggtaaaaact tctggccttc
    33721 gtggtagagg tggtgcaggt ttccctactg gctctaaatg ggaagctgct tcaaaaaatc
    33781 cagctggtcc aacagataaa aagtttgttg tatgtaatgc tgatgaaggt gacccaggtg
    33841 catttatgga tagatctgta cttgaaggtg accctcatag tgtactagaa gctatggcaa
    33901 tatgtggata cgctataggt tctgatacag gatacatata cataagagca gaatatccaa
    33961 aatctataga aagattaaaa gtagccatag ctcaagctga aaaatacggt ttacttggcg
    34021 aaaatatctt aggcactgga tttaacttta aattagaatt aaaatatggt gcaggtgctt
    34081 tcgtttgtgg tgaaggtaca gcgctaatgc attcaattga aggtagacgt ggtgaaccta
    34141 gaatgaaaac ttatagttct tctaagagtg gtctatggaa atctcctact tgcttaaata
    34201 acgttgagac ttttgctaac atacctgcaa ttatacttaa gggtggagat tggtttgcca
    34261 acttaggtac tgaagattct agtggtacta aagtctttgc tcttggtgga aaagtagaaa
    34321 atgtaggact tgttgaagtt ccaatgggta ctactttaag agatattgta tttgaaattg
    34381 gtgggggaat ccctgaaggt aagaaattta aggctgttca aacaggtgga ccatctggcg
    34441 gatgtatacc aactgaacat ttagacactc ctatagactt tggttcatta agttctatcg
    34501 gttcaatgat gggctctggt ggtatgcttg ttcttgatga atctgactgt atggtagata
    34561 tagccaagtt cttccttgag tttactgttg aagaatcttg tggtaaatgt acaccttgtc
    34621 gtataggtac tacaagatta ttagaaatat taactaaaat tacagaaggt aatggtactc
    34681 tacaagattt agatgaccta gaaaaccttg ctgaaactat acaaactgct tcattatgtg
    34741 gtcttggtaa agctgctcca aaccctgttt taagtactct tcaatatttc aaagatgagt
    34801 atatagctca tgttgtagat aagaaatgtc ctgctggtaa atgtcagaac ttactttctt
    34861 actttataac agatgactgt aagggttgta caaaatgttc tagagtgtgt cctgctggtt
    34921 gtataacagg ttctgttaag gaacaacata caatagatac ttctaagtgt ctaaaatgtg
    34981 gtgcttgtat agataattgt acatttaacg ctataatcaa gaaataactt atttggagga
    35041 ggaataacaa tgagtttagt taatttaact attaatggca aacacgtttc agcacctagt
    35101 ggaacttcta tcttagatgc tgcgaaatta ataaatataa aaatacacaa cctttgtcat
    35161 ttacatatga atgaaataga taagcttgac acttgtgctt cttgtcgtgt ttgtatggtt
    35221 gagacagaga gaggattagt tccagcttgt ggaacagtta taaaggaagg tatgaaagtt
    35281 cagactaata gtgctaaagc attaaatgct cgtagaacta ttgttgaatt attgttatca
    35341 gaccatccac aagattgttt tgtctgtgaa aaaaatggag attgtgaatt acagactata
    35401 gctgctgatt taggagtaag aaaaataaga tatcaaggtt ctaaatcttt tgcaggtaaa
    35461 gatacttcta ctaaatcttt agttaaagac cattctaagt gtatattatg tagaagatgt
    35521 gagactgtat gtaatgacat acaaacagta ggagcattat ctggagttaa tcgtggattt
    35581 aatactttag ttagtacatt ctttaactct gatatggttg aaacagagtg tacattctgt
    35641 ggtcaatgta tatctgtctg tccaactgga gcattaacag aagtagataa tgtacctaaa
    35701 ctttgggatg ttttaaataa aaaagaaaag actatagtag tgcaagttgc tcctgctgta
    35761 agagttgcta ttggtgaaga gtttggatta gaaccagggt caatctctac agggaaaatg
    35821 gtagctgctt taaaagcact tggatttgaa catgtatttg atacaaactt tggtgcagat
    35881 tttactataa tggaagaagc tactgaattt atagaaagaa tccaaaaagg tgaaaatctt
    35941 cctatattaa caagttgttg tcctgcatgg gtaaacttct tagagcacaa ttatccagac
    36001 aagttaaact tagcatctag ctgtaagtct cctcaaggta tgtttggttc tatagctaaa
    36061 aactactatg cacctaaaat attaggaatc aatcctgaag aactttatgt tgtatctgta
    36121 atgccttgtg tagctaaaaa atatgaagct tcaagagaag aattaagtga atctggaata
    36181 ctagatgttg acctttctat aactactaga gagttagcta agatgataaa agaagctgca
    36241 atcgatttac ctaatcttca agaccaagat tttgataatc ctctaggaaa atctacaggt
    36301 gctgcatcta tatttggagc aagtggtgga gttttagaag ctgccctacg tacttcatat
    36361 gagaagataa ctaataagac tttagataat gtaaacttca ctaatgttag aggtcttaaa
    36421 ggaattaggg aagcaagtat agatgttgat ggaactacag ttaatgtgtg tattgtaaat
    36481 acacttaaaa acgctagaaa gattatggat aaggtcagaa gtggagaatg taaataccat
    36541 atcatagaag ttatggcatg tcctggtgga tgtgttggag gtgctggtca accttatcac
    36601 catggtaata ctgaaatagt tgatagacgt gcaaatgcgt tatatgaaat agatagaaat
    36661 aaggctattc gtaaatctca tgagaatcct gatttacaag ctatttataa agacttcttt
    36721 ggagaaccaa atagtgatgt agcccataaa taccttcata ctcattactt tgataagagt
    36781 tgtgtctatg gagagtgtcc acaagaatgt gcttgtgaag aagctaagta gtcataacaa
    36841 aatataaaat ttattcttaa aaaaggattg tcttaaaata cttttacgta atttaagaca
    36901 atccttatta atttcacaaa ttaaattata tttctaaata gacttattct ttaattaacc
    36961 tatctattaa ttaaagaata cctgccattc tcataagttg ttttgcattt gtagttttac
    37021 atcttccatc ttttatctta tagtcaaaat gaattttatc gtctacatag tactctgaga
    37081 aatgatagtt tacaacttca acatctccac tacctgaaag gtcacatagc tcaaagtcat
    37141 gtgttgtcac catacttata acccagggtt ttgataattt ctttactgcc tcacttgctc
    37201 caataattct atcagcagag ttagttccct taaaaatttc atctactaga accaacattg
    37261 gcagttcttt taaactatat tgaatcattg attttattct aagaatctca gcatagaaag
    37321 ttgatattcc ttgacttaca tcatcttgta ttctcataga agtaaatata gccatacagg
    37381 tcgcatcaaa actcttagcg catactggag cacctgcata tgccagcact aagtttacac
    37441 cgatacttct taaaaatgtt gtctttccag acatatttga acctgtgata atacaagtct
    37501 gagaatttaa ttgtatcgaa tttgctatga cacttttttc tgctattaat gggtggtata
    37561 cctcttcaac tttcaatctt ggagtatctt catattttat tactggaaaa catgtatttt
    37621 cctttacatg agaaacaact gttaaactta ttagagcttc aaattctcca atagattcaa
    37681 accaatctct aatttgattt ccatatatac ctttccatcg ctccaaagct tctgcacaat
    37741 gatagttcca catcaaaaca ccacaggcaa ttatatatag tatctgatta taccttaggt
    37801 ttacagcatt tcctattgaa tttaattgtt taacacctct tgaaactcca cttcccttag
    37861 aaagtttatc ttgtaattct ttcaaatatg cactctcaaa cgtctctttt tctaaaacct
    37921 caaacatttt ttcatatact tgtatacttc tactaaatga aaataatgga gcaagagttt
    37981 gcataagtct tggatatcca aaaccactaa accctaactg gattataaaa agtaaaaaaa
    38041 caggtaaaac aggtaaaaat cctaacatac aaaatataaa agataagatt gttgcagttg
    38101 gtaaacccca agtaaaaata tgcatccatt ttggtatata cacattctta ttttctccat
    38161 attctataaa cgattctata ctactatctg aattgttttt ttgctcttta ccaattatat
    38221 tactgagtgt ttgaatatga atagaaaaat catcttttga taaaagttct tttatagctc
    38281 tctgtctttc taagattaca ttctcatctg gattttcatt ccacaaatat ttggctaaaa
    38341 aatttttccc ataagatgta ttagctacac agatatattg atacaatgat gctcttccaa
    38401 ataagtctaa atcttttgct tgcgaatttt catcttttaa gtattcttta ccatctattt
    38461 taaattcctt ccaaccatct ccaaatctct taatatactt atttaatact tcgctcttac
    38521 ttttaaaata tgtttcttct tcttttactt tactatgaat aactaagagt gctataaata
    38581 ttattattga taaaactcca acaaaaatac ctactatatt tttattgtaa aatccaatag
    38641 caaaccctgc tatcactatc aaaaaagata ttaatcttgc tgtacttatg ttatttgact
    38701 ttttacttaa gagcaattct ttttcagtac tactttctaa aagtttacta aaatattcta
    38761 ttcccataaa aaattccaac tttcttgtta tataatgaaa ctcaaatata aaattaatct
    38821 agtattatta taatctaaca ctgtacttta aattatcctt atataatata tcttattaat
    38881 aaacattata tatatacaac ttgattttaa tttataaggt gtaggacata atcctaatca
    38941 atatttaatt aaatgatgtg tagttcacta ttattcagta ctacttagta tcgattaatt
    39001 ttgataattt tttagtaaat tcttgagctg catcgtatcc cattcctctt tgtctaaaat
    39061 tcattgctgc tacttctaat atcatagcag tatttcttcc aggtttaaca ggtacaacta
    39121 atttctctac attaacacca agtatttctt catattcttt atccagtccc agtctatcat
    39181 aatacttagt ttcatcccaa gcttctaaat gaactactaa atctatcatt ttagaatttt
    39241 tgattgcccc tactccatat aaacttctaa catctattat tcctattcct cttatctcaa
    39301 gaaaatgtct tattagttca ggtgactgac ccacaagcat actttcatct aattttctta
    39361 tttctacagc atcatctgca actaatctat tacccctttg aatcaattct aatgctgttt
    39421 ccgactttcc tatactactt tcacctttta tcagtacacc tataccataa atatctacta
    39481 gcaccccgtg aattgttgtg tgtggtgcta acttattatc caaatagttt gacagtctat
    39541 ttataagtct agtggtattt ctatttgtgc tcaaaagtat tcttttatat tttttagcct
    39601 tttcaattac atctggcttg atttccaagt ctctagaaat tattaacgct ggtatttcat
    39661 atgagaaaaa tctatctagt acttcttccc gaatatgact atccattaat ccaaaaaaat
    39721 tatattctgt ctttccaact atttgtattc tttcatatgc aaaataatca aaatatcctg
    39781 caaactgtaa tcctggtcta tttatatctt gagagtagac ataataatct acatcatcag
    39841 gcatgtatac tacttttaac ttcaactctt ttattatctc tcttattgat actttattgc
    39901 tagtttccat taattttctt cctttctaaa aaaattataa acattatctg caatgctttt
    39961 attcattcca tctacttcta aaagttcttc catagtagct ttttttatag catctacatc
    40021 tttaaagtga tttaataatg atttctttct tttttcacct attccttgta tatcatctaa
    40081 gatagatttt gtcaaagctt tatttcttag acttctatga tatgttattg cataattatg
    40141 aacttcttct tgtatactag caataaatct atataaatta gttgttttat ctagttctat
    40201 ttctttttct ttgcatatca atcctttagt tctatgctta tcatctttgt acattcccca
    40261 aagaggtata tctacatcat tgagttctag tacttttttt actgcactaa cttgtccttt
    40321 tccaccatcc aacaatatca agtcaggtaa atttccatgt tttaatcttc tatctacaat
    40381 ttcagccata gaatcataat cattaggtcc aataactgtc tttattttat atctcctata
    40441 ttccttctta tcttttttag cattcgtata cactaccata gaaccaatcg aatctacacc
    40501 ttgtatattt gatatatcat aagcctctat tcttattggc aacttttcta gatttaatat
    40561 ttgctttaat tcttccaatg ctccaatact tttttcatat tttcttttat tcatatcaga
    40621 aaatttttca agatattcta cagcattttt cctaaccatt tcaactaagc ttttcttctc
    40681 tcccttttga ggaactctta ttgttacttt ttgccccttt ttagatgaca gccattcttc
    40741 taatatagaa ctatcttcta tctcatcttc tataattaat tctttaggta tatactcttg
    40801 ttcattataa aattgtttta caaatgaact taatatagaa gctcttggac tatccattac
    40861 cccttctaat ataaaatgtt ccctacctac aatcttacca tttcttataa aaaatacctg
    40921 gacacaagct tcattatgag ctcttgccat tgcaaccaca tcttggttta agtcacttac
    40981 agtagcatct attttttgtt tctgtatcat ctcttcaagg ctctttatct tatccctata
    41041 tacagcagcc tcttcaaatc taaagttcat agaactctca ttcatttttt cctttaaaag
    41101 ttccatcaat ttttcttctt taccagataa acacattata atctcttcta tcatttttcc
    41161 atactcttct ttagaaacat ttcctgtaca aggtcctaaa catttattta tgtgtagatt
    41221 taaacaaggt cttgtcttat tctttattgc tttatctata tctattttac agcttcttat
    41281 tggatacgta ctacttataa gctccaaggt atcattcaca gcagttatat tggtataggg
    41341 cccaaaatac tttgccttat ctttaagaac tcttctaact tttaagattc ttggataatc
    41401 ttcattcgta gttactttta tatatggata agttttatca tctctaagca ctacattata
    41461 ctttggtctg tatcttttta ttaaattaca ttcaagtatt aatgcctcta actctgaatc
    41521 cgttataata tactcaaact tatatatatt cttaaccata gattttactt tagatgtatg
    41581 gttttttgaa gattgaaaat actgccttac cctatttttt agagatatgg cttttcccac
    41641 atatattata tggtcgtatt tgtctttcat taaatatact ccgggctcag aaggtaattt
    41701 ttttaaatgc tcttgtatat caaacatctc gccctccaaa acattaaatt ttatactaaa
    41761 attataccct aaaatatgaa ttttaaaaag ttatacaatt tccagtgctt taaacaagtt
    41821 aaagcccata ttcccttcta taattgattt gaaaacaatt ttaaccatga agagtaatat
    41881 ggactttact ttgtattact atttacaact tatatttgac taatttactt ataacaaaac
    41941 agtttatcgt atatctgctc taaaattttt cacaatttat ataacttaag aaaaatattt
    42001 ctttaagaat tgacctgtat aagaaccatc aactttagat acttcttttg gtgttccagt
    42061 tgcaactata gttccaccct tatctccgcc ttctggtcct aagtctatta cataatcaca
    42121 agtctttata acatctaaat tatgttctat gactataatt gtatttcctg tatctgctag
    42181 tctttgtaat acttggatta gcttgtctac atctgccata tgaagtccag tagtaggttc
    42241 atctaatata tacaaagtct ttcctgttgg tcttttacta agttcagcag ctaattttat
    42301 acgttgtgct tctcctcctg ataattgagt tgaaggctgt cctaatttaa tatatgaaag
    42361 tcctacatcc attaaagttt ctaattttct ttttatattt ggtatatttt cgaaaaactc
    42421 aacagcttct tccacattca tatctaatat atcagatata gttttatctt tgtattttac
    42481 ttgcaatgtt tctctattgt atctttcacc tttacaaatt tcacaaggaa catatacatc
    42541 tggtaagaaa tgcatttcta tttttattat tccatcaccc ttacaagctt cacatctacc
    42601 accttttaca ttaaaactaa atctaccttt tttataacct cttgctttag cttcattagt
    42661 agatgcaaaa atatctctta taaaatcaaa tacacctgta taagtagcag gatttgaacg
    42721 aggtgttctt ccaataggac tttggtctat atttataact ttatctatac tttcaattcc
    42781 ttttatctcc ttatgcttac cagctctttg cttaagtcta ttcactttac ttgcaacacc
    42841 tttgtaaagt atatcgttta ttaatgtact ttttccagaa cctgaaacac ctgttatgca
    42901 tgtaaactta cctaatggga actttacatc aatatttttc agattatttt ctgatgcttt
    42961 aactatttct ataaaacttc catttccttc tctagtagtc tcaggaagca atatttgttt
    43021 tttaccactt aaatattgac ccgttattga gttctcattt tcaattattt catctaaagt
    43081 accctgtgca actatttcac caccatgtat acctgcacct ggaccaatgt ctataacata
    43141 atcagcttct ctcattgtgt cttcatcatg ctctacaacg attaaggtat ttcctaaatc
    43201 tgttagatgt ctaagagtag ttataagcct atcattatct ctttggtgaa gccctatact
    43261 tggctcatca agtacatata aaactccaac caatgcagaa cctatttgag tagctaatct
    43321 tatacgttgc gcttctcctc ctgataaagt tccagctttt cttgataaat ttaaataatc
    43381 aagacctaca tctcttaaga acatagctct tccttttatc tcttttataa tctcatgagc
    43441 tataaatctc tgtttttctg taagatttat attttccata aagtcaatca attcatttac
    43501 agataagttt gtaacttcca ttatattttt tccatcaact aaaacagata aaacttcctt
    43561 tttaagtctc atccctttac actttggaca tggtctctct gccatgtatt cttcaatctt
    43621 atctctcatg taatctgaat tagtttctct atatcttctc tctaagttta ctattacacc
    43681 ttcaaagtat gatttataat ttctagttcc tccaaatttt gactcataag taaactctac
    43741 aattatatta tctttaccat aaagtaattc ctccacaaaa tcatctggta aatctttaaa
    43801 cggtgttgtc aaagatacat taaaatgctc tgctaaactt tttatcattt tactgtaata
    43861 agtgtcatca ttaacacctg ttgaacccca tgctgccact gcaccttgtt ttattgataa
    43921 atctttgttt ggtactacta attctgggtc aacttcttta ctttccccaa gaccattaca
    43981 aacatcacaa gcaccaaagg gagcattaaa tgaaaacatt cttggagata attcttctat
    44041 gccaattcca tgttctgggc atgcaaattt agttgaaaaa agaatttctt ctccatctat
    44101 tatttgggct attacaagac catcagatag cttaactgca gtttcaattg aatctgctag
    44161 tcttgattct attccatctt taattactat tctgtccact acaacttcta tattatgttt
    44221 ttgattttta tttaagatta tatcatcact aacttcatag ttttcaccat taactctaac
    44281 tcttacaaat ccttcttttt tgatattatc aaagactttt ttatgtgtac ctttttgtcc
    44341 tctaacaatt ggtgaaagaa tttgtaattt cgttctatct ggaaaatcta gcattttatc
    44401 aactatttct tgaatagtca tctgacttat tttttcacca catgtagggc aatacacatc
    44461 tcctactctt gcaaataata gtctcaaata atcatatatt tctgttactg ttccaaccgt
    44521 agaacgtggg ttttttgagg tagtcttctg gtcaattgag atagctggtg ataaaccctc
    44581 tatatattct acatttggct tttccatctg acctaaaaat tgtcttgcat aagcagatag
    44641 actttctaca tatcttctct gtccttctgc atatattgta tcaaatgcca aagaagactt
    44701 cccagaccct gacaaacctg taaaaacaat aaatttatct cttggtaatt ctaaatctat
    44761 attttttaaa ttatgttctt ttgctcctct tattataatt ttatcttcca taatgttgtt
    44821 tttacctttc tatttttcca atttttcttc taattgtttc actttatctc taagttctgc
    44881 tgctctttca aattgtaagt tttgagcagc ttccatcatc tctgatttta atttatctat
    44941 attagctctt atttcgtctt tatcatctgt atcagatatt ccatatacaa cttcttcctc
    45001 tgcaacctta gtagcttcta tactatctct tatatttttc tgtatagttt taggaatgat
    45061 attgtgctct tcattatata aattttgtat atctcttctt ctctttgttt catcaatagc
    45121 attttgcatt gagtcagtta tcctgtctgc atacataatt actcttccat tttcatttct
    45181 agctgcacgt ccaatagtct gtattaaagc tgtttcggaa cgtaagaaac cttctttgtc
    45241 tgcatctaaa attgctataa gtgaaacttc tggtatatcc aatccttctc taagtaagtt
    45301 tattcctaca aggacatcaa acttccctaa tcttaaatct cttattattt ctgttctttc
    45361 taatgtaact atatctgagt gtaaatattt aacttttatc cctatttctt taagatagtt
    45421 agtcaagtcc tcactcattt ttttagttaa agtggttata agcactcttt catttttttc
    45481 tatagtttta gttatttctc ctaccaaatc atctatttga ttatttatag gtcttacttc
    45541 tacaattggg tctaaaaggc cagttggtct tattatctgt tctgctattg tctctgagtg
    45601 ttgaatttca taaggccctg gtgtagcact tacaaacaac atttgattta tatttccttc
    45661 aaattcttga aaatttaatg gtctattgtc aagtgcagaa ggtaatctaa aaccattctc
    45721 tataagtgat gtttttctag acctgtctcc tgcatacata cctcttactt gtggtatagt
    45781 gacatgtgcc tcatctacaa ttatcaaata atcatctggg aagaaatcca ttaaagtata
    45841 tggtctttct ccttctgacc ttcctgtaat atgtctagaa tagttctcta tcccctgaca
    45901 aaatcctatt tcctttaaca tttctatatc atacttagtt ctttgttcaa ttctttgtgc
    45961 ttctaatagc ctatcatttt ccttgaaaaa tttaatttgt tcttgaagct cgttttctat
    46021 ttctacaatt gccttttcaa ttctttctgg cgttgttaca tagtgagatg ctgggaatat
    46081 aacaacatga tttctagtac caacaatctt ccctgttaca taatcaattt ctgttattct
    46141 atctacctca tcaccaaaaa attctattct tattgctttt tcatcattac tagctgggaa
    46201 tatttctagt atatctcctc taactctaaa ggtacctcgt gtaaagttta tgtcatttct
    46261 ttcgtattgt atctctatta acctttttat tacatcatct ctgtctcttt gcatacctgg
    46321 tcttatagat aacataagtt ctttatagtc ttttgggtca ccaagtccat atatacaaga
    46381 tacagatgat attattatag tatctcttct ctctaaaata gatgctgtag cagaatgtcg
    46441 aagtttatct atttcatcat ttatgcttgc atctttttct atataagtgt cactgtgagc
    46501 tacatatgct tctggttgat aataatcata gtaacttaca aagtattcaa ctgcattgtc
    46561 tgggaaaaat tctttaaact cactgtaaag ttgtgctgct agagttttat tatgagccat
    46621 tattagggta ggtttcttaa cttgttgaat gatatttgcc attgtgaatg tttttcctga
    46681 acccgttaca cctaataatg tcgaaaattt ctcatttcta tttatagaat ccactattga
    46741 tttaatagct tctggttggt cacctgtagg tttgaagtct gattttattt taaaatccat
    46801 agtatcactc cttttttcga acattagttc ctgacatgta ttattatatc atacctttat
    46861 aaaattgttt aagttatttt aaatatttta cccatttaaa ttttacttaa atctaaaaca
    46921 taaaaaaaga cttctatctt tgcaatagaa gtctataatt catggaaaat aaatacttta
    46981 ttcaacttac ataataaaat caaatgcttc tttttcaact ccatctaatt ttaaattaaa
    47041 catttttaat atagttgctg catgtgctct caggtctcct tcttctataa ccacaccacc
    47101 tttaatattt ggtccaaaag ctataaatgg aggtctatta cctttagtag gtaaatgacc
    47161 atgtgtcgca acagcaaact tgtaatcttt tatatctgaa tcttttataa tacttccttc
    47221 gaaatcattt ccaaatgaaa ttccttcaag accttcaatt accatttcaa aatcaccttc
    47281 aagaccaagg ttagatgctt cctctttggt aaaaatattc tctatgaata attcttcttt
    47341 aaattcttca agcagttctc ttaattctgt taacctatct aagttattta ctattatttg
    47401 agctgatatt ccagaagaat ttacataggc atcaaaatcc ttaacttctc cattttcaac
    47461 ttttataaat ccatttgatt taagcaagac atttggattt ataactttat caactctaag
    47521 atgaccatgg tccccaagta ttataaaatt tgtatctttg tatgtacctg catcctttgt
    47581 agcctgaata atatctccta accatttatc attcattttt aatgctttat ctacttcgtc
    47641 attaaataag ccataattgt gtcgtgtatg gtccaatgta gctaaatgta ttagcattaa
    47701 atctggttta tattccttta ttatatctac tgaacagttt actccaaata aatccatgtt
    47761 gggttctaat ttccaatcga tataatgaca atgtctattg taaataccgt ccattataac
    47821 cttactggaa cttcgttcaa aagtttctct tgggtcatct tctctactag gagcccatat
    47881 ttctgcaata ttataatcta tatttggatt tgctcccatt acaggccata atacactaga
    47941 ggtagttaga ttattttctt ttgctacatc tataatagtt tttgctttta tgtcctttga
    48001 gtaccagtac cagtctttat tttctttatt tgggtcaaat ttttcattat gatatatacc
    48061 atgtttttta ggatatactc ctgtaaccat actagtatga cacggatatg tcagagtggg
    48121 ataaacacaa tttatatttt ttactatgga gcagttttct agtatagatt taaagtttgg
    48181 tagatttttt acaaattcca agtctttttc aaataaagaa tctactgata atactataac
    48241 ctttgtcatt tacattacct cttcatttat atattattga atacttttaa tcaacttaca
    48301 agtctatata attatttatt tgttttatct attactgctt gcattgcaga agttcctttg
    48361 cttactacat cttttggatt tgcatttaaa tcagtaagcc cctttttaat ctcatccatt
    48421 agcttatcag ttgcttcctt gtatccattt aacattggaa gtgcaacagc atattgtaat
    48481 tggtcagttg caaccttata ttgaggtttt tctttatata atgtttgtaa cttttctgag
    48541 tttgttgcat cttcagttgt tggtaagtat cctgttgatg aactaaactg aacgatttta
    48601 tctgtttgtg tcatccattc tataaattct gcacttgctt ctttttcttt atctgttcca
    48661 ccttcaagca taactagatt agctccacct gtaggagttg cgtatttttg attttttggt
    48721 aagaatgctg ttgatatttc aaacttacct tctgtttgtt gcataagtcc tgctaaatct
    48781 gctgttgatg taaatatcat acctacttta ccatttacaa aatcttgttt tgctacgtcc
    48841 caggcattat aatcttctcc aggaggcatt ttcattattc cagctttcat catatcttgc
    48901 cagaacttaa ctggtgcttg tcctgcctca ttattaaatg ctactttttt accattagtt
    48961 atttctcctc cactttgcat taccatagct tcatagaacc aaatatctat tggtgtttca
    49021 aaaccataag tatcttctga tttatttgtc attttactag cataatcctt aagctcttcc
    49081 catgattttg gacctgaagg gtcaagacct gctttagttg ccatatcttt atttatgtat
    49141 aatattggtg tacttctatt aaatggcata ccaacaaact tatcctcata atatgaattt
    49201 gttaaaagac cagaaaagaa tctttcttga tatgttttat caaaatattt actcatatct
    49261 actaaagcac ctgctgatgc gaatgtacct gtttgagtta tttctaataa tgttacatct
    49321 ggttggtctt tagatgttaa ggctgcctgt agttttgttg cattttcata gtaatctttc
    49381 tgttttaaca tatttacttg tacatcctta tgttcactat taaattggtc aaccattttt
    49441 tgagcagttt ctccattagt tccacctaaa ggagcccaga aatctatctt cgtaatattg
    49501 ctatcagtct tttctccttt agatgcattt gcatcattag aatttgaaca cccaactaaa
    49561 gaacctaata ataatgttat tgctaatcct gttgctaatt ttcttttaag cttcattttc
    49621 atcctccaaa tttagtcttt aattttttat tttattcctg aatacataaa agcttctttt
    49681 acttttttat tcgcaaatat ataaacaaga attattggta aaattagaat tatatttcct
    49741 gccattatta cattccatgc agtaattcct tctgaagatt taaggagcgc aactcctatt
    49801 ggtaaagttc ttattgcatc tgtatttgtc attactaagg gccagaaata attattccaa
    49861 tggtatataa aattaaataa tataaatgtt attactgctg gttttgccat aggaaccatt
    49921 attctaaaca ttatttttaa gtcagaagca ttatctagtt tagctgcttc aataatttca
    49981 tctgaaatct gcataaaatt ctgtctaagc agaaatatcc caaatgaaga tgtcataaat
    50041 ggaattataa gtggaacata agtatttata aatccaaact tactcatcat catatagact
    50101 ggtagaaaag ttacttgtgg tggaatcata agtcctacaa gcaaaagccc aaatagcatt
    50161 ttttcacctt taaatttaat cttagaaaat gcatatgctg ctggaaccat aactaaaaat
    50221 tgtacaatta aaacacttcc tgttacaaat acactgttta gcaaatatct aaaaaatgga
    50281 cctgaatgcc atgctgtact ataattttct aacactatat catttggtat aatagtgggc
    50341 ggtaattgca tcatttcttg tggattttta aaggatgtag atagcatcca tataaaaggg
    50401 aatataaata ttgcagataa aagtaataga attaatattt ctattgattt tgatgctatt
    50461 ttttttatat caactttgtc ttttttgata ggttgtataa tatccattgt ctcctccttt
    50521 tggataaacc ttataatatc tactttttct ttattgataa tgaactttct ttgataagag
    50581 tctaaagtta gcatatgtag caatcataac tatagctaac aaaaacatac tcgctgcact
    50641 cgcttgccct atgtcatagt atataaatcc agactcatat atataataag ataacatgtt
    50701 agttgaaccc actggcccac cttgtgtcat tatacttatc tcatcaaata cttgaaaaga
    50761 tgcagttgtt gttgttatca taagaaaaaa tattgttggt gataatagtg gcaatgtcac
    50821 tttaaagaaa ttcgttattt tgtttgcatt atctaattta gatgcttcat atatatagtt
    50881 tggtatggat ttaagacctg ataacactat taagatatta tatccaagag tcttccatat
    50941 tcctattaga attagtgaca ttaaagctga actttcactt gaaagccact ttgatgttgg
    51001 taaatgtaat gtcgatagta tccagtttaa aaagccatat tgtggctcca taatccaaag
    51061 ccacaataca gctacagata ctagtgaaat tacatgtggt gtaaagatta tactttgcgt
    51121 taaattgtgt attatagaat ttttatttag ccaaacagca agtactatgg ctagtgatat
    51181 agatatagtc accataaaaa aagtataaat tgcagtgttt gttatagttt gtataaactc
    51241 tggattcttt aaaagttctg tataattgtt taagcctaca ttttcaaagg ttgaactaag
    51301 aagactacca gatttgaagc ttagagatat cgtcttaatt attggataga ctataaacaa
    51361 taaagttcct ataatagcag gtgttatata taaatatgct ttattaaatt gctttttcat
    51421 aaatatctcc tttaatttta gaatacatac ttttacatac ttttacatct tctattcttt
    51481 ctccattttt atcaaaataa tataaatctt tttctaatac acttattctt aaagttcttc
    51541 caatatcaaa tatatcattt tctgttttga ctcttatctc tctattaaga acagataaac
    51601 aatatatagt ttcatttccc aacacttctt tagttacaac ttttccaaat agactaaata
    51661 catcatcatt ttttccatct atgtttatac tctctagtgt tgctttttct ggtctgaatc
    51721 caaatttaat atcatttttt atatctagta tgttcataca aggactccct ataaattgag
    51781 ctacaaataa atttttagga ttttgatata tcttctttgg agaacctatt tgcattatct
    51841 ttcctttgtt cattataact atcttatctg ccattgacat tgcctcaacc tggtcatgag
    51901 ttacataaac aaatgtagac tttagttttt gatgtaattg tattaattct gttctcattt
    51961 gatttcttaa tttagcatct aaatttgaca atggttcatc cattaaaaat acctttggat
    52021 ttttaaccat agctctagca agtgccactc tttgtctttg acctccagat aagttgcctg
    52081 gtttcacatt taaatattct ctcaatccta ctacatcaat aacttctgtt ataagttttt
    52141 ctcttttttc tttttctact tttttgtttt taagaccaaa ttctatgttt tctctaacag
    52201 tcatatgtgg atataatgca taattttgaa ataccatagc tatatccctt ttactagcat
    52261 ccgtttttgt aacatcattt tcttcaatct tcaacctacc tgaagttata tcttcaagac
    52321 ctgcaatcat tcttaacgta gtagatttac cacatccact tgggcctacc aatactgtaa
    52381 aagaaccatc ttctatagta agatttaaat tttctataac tgtatttttt ttattataag
    52441 attttgttac attttctagt cttatatctg ccatttttat cctcccaaaa tatttaattt
    52501 aattctacga tttacttata gattaataat actcttaatt tgtaaaggat atattaagac
    52561 aaagtgaata tatggtaaac ttattaaaat aaaggttaaa ttataaacat tttttgttaa
    52621 atatactgag gttaagatga tatacagctt atgttagcta taattttcta tatttttcat
    52681 attcattgtt tgctatcaca atgtaatttt aatttatgaa gagtcatata aaactattta
    52741 ttttttatat aatttgattt gaaagtaaaa aagacctcta tgggggatag aggtctttaa
    52801 gtgggggaga ttgagtaatt tatttttgct atattaagtt ttacaattat attatgtccc
    52861 aaatttacaa ggtttaaaca taaaaaataa aaatttttta atttttataa atttacc
//
LOCUS       DAEPIN010000021        50889 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.021, whole genome shotgun sequence.
ACCESSION   DAEPIN010000021 DAEPIN010000000
VERSION     DAEPIN010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 50889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50889
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.021"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            complement(8..214)
                     /locus_tag="KR250_001447"
     CDS             complement(8..214)
                     /locus_tag="KR250_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0067394.1"
                     /translation="MELIEKVSINSLSKRDLLLIIKSLEYTNENTQISDFIDLRNNIV
                     KELCFLTDTKEKDFLDYLEKNSTL"
     gene            350..2104
                     /locus_tag="KR250_001448"
     CDS             350..2104
                     /locus_tag="KR250_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HBH0067395.1"
                     /translation="MKILLTTLNSKFIHTNLAIRYLKEFVRDLIEVNMKEYTINNDLD
                     YILKDIYKNEYDIILFSTYIWNVGDIVKLCDNLKKIRPNTKIALGGPEVSYDSYEAMK
                     KYDFVDYILYGEGELIFRDLVLHLQGKMEINDVNGLVYRQGNEIIVNKPMELLQDLDE
                     IPSPYENLNPKEYENRIVYYESSRGCPFNCQYCLSSTIPGLRYFSLDRIKSDLKALID
                     ARVSQIKFIDRTFNANRKVAMEIMDFLMKNDNGYTTYHFEVTAYLIDDKMLEFLADCK
                     EGLFQFEIGVQSTNEKTLDAVGRRDDFKKLSHVVQTVASYRNIHQHLDLIAGLPYEDY
                     KSFENSFNDVFNLGIEHLQLGFLKMIKGTGMRKVADEHGFKYKDYAPYEFLYNNYISY
                     EETLKLKDIEDILERYYNSKNFVLSMRYIIGRFYKKSPFKFFEVFAKYFDENGYFDLA
                     QGKNQLYKILMDFYNEKINIDNDVFNDILKYDYISLGKTSNIPQFFNKLDVDDFKNRC
                     HVFLQDNDNLSTYLPSFVDKPAKHIIKYVHFEPFRFNVVDLKNDINTEIREVENVVLF
                     EYDDKKIFEKSKTHKVEI"
     gene            2122..3264
                     /locus_tag="KR250_001449"
     CDS             2122..3264
                     /locus_tag="KR250_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBH0067396.1"
                     /translation="MDFKIEKYLLKKAEELAFITIKHGGEFKLKSYEVPKGGLDVPIK
                     NEVLVKGIKEKTAQDKLNSMSIADAMIYIIGIDSKFKNNAEYEKFLNALSKDIDLDLK
                     SYMGYMSRKYFEIGEHTDSLIYIKAFITMYPDDLDAMYNYAIVCQEIAKQYQKDMDDK
                     AMNAFLLEAMAKLEKVIDVDENFALGYYHLGYHYYNQGQYLKTKLTWEEALRLGLDAD
                     LVAEVQENLGKMDFKVQYEEGYTLVFQGKFKEGLEKLLPLEEEHMDWWNLLFMIALGY
                     KGMGEIEQAKMYLEKILIIKPNQVDTIVELGLCEAYKNNLDKAIEYFEQAAKIKEDPE
                     ILCNLGMAYLNNGDIDDATYYIERAYELNPQDEITIACLRELGINK"
     gene            complement(3436..4359)
                     /locus_tag="KR250_001450"
     CDS             complement(3436..4359)
                     /locus_tag="KR250_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBH0067397.1"
                     /translation="MEIRVLRYFLAIAREESISGAATFLHITQPTLSRQIMELEEELG
                     VKLLVRGNRKITLTNEGRLLHKRAEEIISLVDKVELEFDNPNEIISGDIYIGSGETEA
                     MRLIARTAHHFHQKYPNVRYHLFSGNADDVTEKLDKGLLDFGILIEPANIQKYDYAQI
                     PATDVWGLLMRKDSALASQSTINPKDLCNIPLIISRQTLVDKAISKWLQGSNEKLNII
                     ATYNLVYNASLLVEEGLGYALCLDRLVNTTENSNLCFRPLSPPLEAKLNIIWKKHQIF
                     SKAAEKFLLQIQKDFSSIMLDTSKLDTPKEK"
     gene            4585..5163
                     /locus_tag="KR250_001451"
     CDS             4585..5163
                     /locus_tag="KR250_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HBH0067398.1"
                     /translation="MNINEFLTHINQRKPVSSKSEIHEFMVKLSNEAMKITSKLNSSY
                     HEPEEVRSLFSELIGKKVDESFFMFPPFYTDCGKNITIGKNVFINSSCHFQDQGGIEI
                     GDNTQIGHNVVLATLNHGIAPEKRGTTYPSPITIGKNVWIGANVTVVPGVTIGDNAII
                     AAGAVVTKNVAENTIVGGVPAKLLRAIDEIPE"
     gene            5279..6190
                     /locus_tag="KR250_001452"
     CDS             5279..6190
                     /locus_tag="KR250_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HBH0067399.1"
                     /translation="MIKKLDDILEQLKGGEKIVLSVAAAHDKEVLMAIKDAVERNIIT
                     PILVGNEGKIREISKEIGFDLSGIKIVDKDDIKECAEIAVKLVSSKEADFVMKGLLDT
                     SVILKEVLNKDYGLRTDSLLSHVMVYELEKYHKLLITTDGGMNIAPDYEQKAKILKNS
                     IKAAKALGMETVKVACLAAKEKVNPKMQATVDADMLAKACKEGEFGENVIVEGPLAFD
                     LAVSKEASEIKGFKSEVSGDVDIILVPTIEVGNGIGKAFTYMADSKSAGIIMGAKAPI
                     VLVSRADSHESKLYSIAYGAIVAKNMK"
     gene            6797..8716
                     /gene="cooS"
                     /locus_tag="KR250_001453"
     CDS             6797..8716
                     /gene="cooS"
                     /locus_tag="KR250_001453"
                     /EC_number="1.2.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic carbon-monoxide dehydrogenase
                     catalytic subunit"
                     /protein_id="HBH0067400.1"
                     /translation="MDEKMLTIDLNSQKMIAKAREEGVETMYDRKEGFKAQCGFGLQG
                     VCCRICGMGPCRISPKTPRGLCGADEHTIVGRNFARMVAGGTAAHSDHARDIAHTLAL
                     ADPNGNYKIRDEAKLITLAKEWDVETEGRDIYDVAHEVAEIALMEFGKPFGTLRFIKN
                     APEPRQKIWKEYAIEPRAIDREIATIMHSTHIGCTGDIDSLIHMSLRTSMADGWGGSM
                     IGTRLSDILFGTPVPRRTEANLAVLEENKVNIILHGHEPALSEMIVLASEEPDLVALA
                     KEVGADGINLAGMCCTGNEITMRHGVKIAGDFHQQELAIITGAVEAVIVDVQCIFPAL
                     ARVADCYHTKFVTTSPKAKITGSTYIEFREEQALDDAKAIVKEAILNFKNRDKSKVLI
                     PELKSGATVGYSVEAVVNQLDRVVNSHIDPAGTVKPLTDCLKAGVLRGAAGVVGCNNA
                     KGVSNEAHVTIMKELIKNDIIVVTTGCGASAAAKFGLMESDAAEKYAGKGLATVCKLV
                     GIPPVLHMGSCVDISRILDLVGAAANYLDMDMCDLPVVGIAPEWMSEKAVAIGCYVVA
                     SGIDTYLGIMPPIAGSSRAVDILTSELKDKVGATFTVNTNPKELAATIIEDIEKKRVH
                     FEALVEEKMAEKAEA"
     gene            9026..9805
                     /locus_tag="KR250_001454"
     CDS             9026..9805
                     /locus_tag="KR250_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBH0067401.1"
                     /translation="MKIAITGKGGVGKTTFSSMLSRMFAEDGYRVVAVDADPDANLAL
                     ALGFPKEVYESIVPISEMKKLVSDRTAASVGSFGKMFKMNPKVDDIPENFCKEYNGVR
                     LLTLGTVDSGGTGCVCPEHVLLKRLCSHLILQNKDVVVMDMEAGIEHLGRGTAQGVDA
                     FIVVVEPGERSLQTYRKVKKLGHDIGVNKVFVVGNKIRNKEDEEFIIQNLEDGESLGF
                     IYYNQDVIDSDRANQSPYDSSETTKEQIKAIKDKLMSLKDK"
     gene            10054..11730
                     /locus_tag="KR250_001455"
     CDS             10054..11730
                     /locus_tag="KR250_001455"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014355320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HBH0067402.1"
                     /translation="MGFKSDIEIAQEAKPQDIREVAKKLGLGEDDVELYGKYKAKVDY
                     NLLKRETGKKAKLILTTAINPTPAGEGKTTTTIGVADAFAKLDKNVLVALREPSLGPV
                     FGVKGGAAGGGYAQVVPMEDINLHFTGDFHAIGAANNLLAAMLDNHIHQGNALRIDPK
                     KITWRRCVDMNDRQLRNIVDGMGKKGDGAVRQDGFDITVASEIMAAFCLASDISDLKE
                     RLGNIIVGYSYEGEPVTARQLKANGAMAALLKDALKPNLVQTLEGTPSFVHGGPFANI
                     AHGCNSVIATRMAMHFADYVITEAGFGADLGAEKFLDIKCRMANLKPDAVIIVATVRA
                     LKYNGGVAKADLNNENLEALEKGLPNLLKHVENITQVYGLPAVVAINRFPLDTEAELK
                     LVEDKCKELGVNVALSEVWAKGGEGGIAVAKEVLRLLDEEENNFRFCYEDDLSIKDKI
                     NAIATKIYGADGVDYTPEADKEIANLEKLGFTKVPVCMAKTQYSLTDDQTKLGRPTGF
                     RITVRQATISAGAGFIVALTGEIMKMPGLPKVPAAEKIDVDENGVIAGLF"
     gene            11835..12467
                     /locus_tag="KR250_001456"
     CDS             11835..12467
                     /locus_tag="KR250_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HBH0067403.1"
                     /translation="MKIADKTCVDFVEVLSSKEAVPGGGGAAALVGAIGMALGSMVCN
                     LTIGKKKYAEYEESVKEILSKAGKLEKDLLKMIDDDAECFLPLSKAYGLPKETEEEKR
                     IKAETMEKALKVACEVPLNIVRVCYEAIKLHEDLVDKGSRLAISDVGVGVQCLRAAIL
                     GGQLNVVININSIQDKEYVNKVKTEVDKLVEDGVKICDEVYAKVEKALGK"
     gene            12559..13431
                     /locus_tag="KR250_001457"
     CDS             12559..13431
                     /locus_tag="KR250_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylene-tetrahydrofolate
                     dehydrogenase/5,10-methylene-tetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HBH0067404.1"
                     /translation="MEGMSTKGQIIKGKPVADKISEELIKEVDLLIKEGINPKLTIVR
                     VGARSDDLSYERGALKRCQNIGITTEVLELAEDITQEEYIDVLKRVNDDKNVNGILCF
                     RPLPKHLNEEVIKYVIAPEKDVDCFSPINSAKVMEGDKSGFPPCTPTAVVEILKHYNV
                     DLKGSKVTVLGRSMVVGKPVSMLLLSEHATVTICHSKTKNLSGVAAEADVLIAAIGRA
                     KMVDESFVKDGAVVIDVGINVDEEGNLCGDVDTNAVLDKVSMITPVPAGVGSVTTSIL
                     AKHVVKACKLQNNK"
     gene            13468..14142
                     /locus_tag="KR250_001458"
     CDS             13468..14142
                     /locus_tag="KR250_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HBH0067405.1"
                     /translation="MIISENKPLEEVLGYLKDFDKLVLVGCNQCAATCKSGGEEEVLK
                     MKETLEGEGKKILGYVMLDPACNLLKSKKDLKALKEETKEADAVLSLACGDGTQTIVK
                     NLKDKPVYPANNTLFIGEVQRVGEYEEACKACGDCELGWTGGICPVTMCAKGLMNGAC
                     GGAKNGKCEVNSENDCAWIKIYERLEAIGQLDNLAEIRPPKDYSKQNNPRSLSAKKKK
                     EAAANS"
     gene            14178..15059
                     /locus_tag="KR250_001459"
     CDS             14178..15059
                     /locus_tag="KR250_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HBH0067406.1"
                     /translation="MSLLRETLESGKFAVTTEMAPPKGTDLSHLIECAKPLVGRVHAA
                     NVTDFQSAVMRATSLATCKLLKDAGLEPVIQITGRDRNRIAIQGEMLSAGVFGINNLL
                     ALTGDHTSVGDHPQAKGVFDLDSVGILQTAETLMAGTDMAGNKLKGSPDFYLGASVTP
                     EYSPIEVQLLKMKKKIKAGAKFFQTQALYDINTMRKFRELTRDMDCKVLAGIVPLKSP
                     GMAKFMTANVPGIFVPDEQIERLRAAGKENWVSEGIKMAGELIKQLKEEDLCDGVHIM
                     AIGAEENIPAILDAAGL"
     gene            15103..16488
                     /gene="lpdA"
                     /locus_tag="KR250_001460"
     CDS             15103..16488
                     /gene="lpdA"
                     /locus_tag="KR250_001460"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HBH0067407.1"
                     /translation="MKIVVVGGGPGGYVAAIKASMLGADVTVVEKRRVGGTCLNAGCI
                     PTKALLASSGVLNTVKEAKDFGIEIDGTVKPNFTAIMERKNKVVNQLISGIEFLFEKR
                     GVNLVNGFGKLIDKNTIEVTKDDGTVETIKADKIILANGSVPVVPRMFPYDGKVVITS
                     DEVLGLEEIPESMLIVGGGVIGCEIGQFFRALGTEVTIVEMVDQILLNEDKDVAKQLL
                     RQFKKDKIKVITGIGVQTCEVVDGKAVATLSNGKVIEAQYALVCVGRRPNLDNSGVED
                     IGIEMERGKVVVNEHLETNVEGIYAIGDIIDTPFLAHVASKEGIVAVENALGKTKVVD
                     YRAIPRCVYTEPEVAGVGKTEKQLEAEGVEYNVGQFDFRGLGKAQAIGHFQGFVKVIA
                     DKETDKIIGAAVVGPHATDLLTELSLAVHLGLTVEQVGDAIHPHPSLSEGLMEALHDV
                     HGECVHSVPKL"
     gene            16544..17314
                     /locus_tag="KR250_001461"
     CDS             16544..17314
                     /locus_tag="KR250_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBH0067408.1"
                     /translation="MGYNIAVAGKGGTGKTSLTGLLIDYLVKDKKGPVLVVDADANAN
                     INEVLGIEVEATIGEIREEVNQREKLGNAFPGGMTKAQYLQFRLNSIIEEGEGYDLLV
                     MGRSEGEGCYCFVNGILREQVNKISGHYKYLVMDNEAGMEHLSRKVTRHVDTLLLVSD
                     CSRRSIQAVARIRDLAEELKLSVGRILLIVNKVPNGVMNDGVKEEIEKHNLELIGVVP
                     MDELIYEYDSTGIPLVNLPEDSKSKVAMKEIFAKLELK"
     gene            17334..18278
                     /locus_tag="KR250_001462"
     CDS             17334..18278
                     /locus_tag="KR250_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit delta"
                     /protein_id="HBH0067409.1"
                     /translation="MAFKMSTQKYSGKISEVEVGIGEKAIKLGGENVLPFYSFDGEVG
                     NSPKIGIQISDVYPESWTDSYKELYKDVANCPVEWAKYVEANTQADFICLKFDGSDPN
                     GLDKSVDECADVAKAVIEAIKLPLVVAGSGNHEKDGKLFEKLAQTLDGHNCLFMSAVE
                     DNYKGVGASAGMAYAHKVGAESSVDINLAKQLNVLLTQLGVKGENIVMNVGCSAVGYG
                     YEYVASTMDRIRLAAFGQNDKTLQMPIITPVAFEVGHVKEAIAPIEDEPDWGCPEERT
                     IAMEVSTAASVLVGGSNAVILRHPKSIETIKELVNALA"
     gene            18309..19676
                     /locus_tag="KR250_001463"
     CDS             18309..19676
                     /locus_tag="KR250_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit gamma"
                     /protein_id="HBH0067410.1"
                     /translation="MALKALDIFKLTPKKNCKDCGFPTCMAFSMKVASGAVEVGKCPH
                     MSDDAIAKLSEATAPLMKALKVGAGASEYELGGETVLFRHEKTLVSRNRYAVSFCTCM
                     SDEAVDAKIANMKKVDYVRIGEQMKVEMAVLEYCGDKDAYLKLIDKIKGSGLEVAYIL
                     ACDDAQVVKEAVEVLKDARPMVYGATKENYKDMIEVVKGASLPLGVKAGSLEELYETV
                     ELIQAAGYKELVLDVTGENIKDTYTNAIQVRRTALKEQDRTFGYPSIVFANRLSNSNP
                     MMEVALSSIFTIKYGSIIVIDDISYAKALPLFALRQNIYTDPQRPMRVEPKIYPINNP
                     DENSPVLVTVDFALTYFIVAGDIERSKVPVWLVIPDAGGYSVLTSWAAGKFGGNSISA
                     FIKESKVEEVTNCKDLIIPGKVAVLKGDIEDNLPGWNVVIGPEESMELPKFLKGYQEK
                     ACQTN"
     gene            19726..20532
                     /locus_tag="KR250_001464"
     CDS             19726..20532
                     /locus_tag="KR250_001464"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HBH0067411.1"
                     /translation="MEKFMIIGERIHCISPSIRKALAERDPAPILKRAKEQLEAGAHY
                     IDFNIGPAERDGEEIMTWGIKLLQSEFNNVPIALDTANKKAIEAGLKVYDRTNAKPII
                     NSADAGSRFDLIDIAAEYEAMVIGLCAKEGIPRDNDERMAYCQEILEKGLMLGMEPTD
                     ILFDPLCLVIKGMQEKQVEVLEAIKMMTEMGLLTTGGLSNVSNGCPKHVRPVLDSAFL
                     AMAMANGFSSAIMNPCDPELMKTVKSCDIINGASLYADSFLELNEGGFAF"
     gene            20589..22715
                     /gene="cdhC"
                     /locus_tag="KR250_001465"
     CDS             20589..22715
                     /gene="cdhC"
                     /locus_tag="KR250_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CO dehydrogenase/CO-methylating acetyl-CoA
                     synthase complex subunit beta"
                     /protein_id="HBH0067412.1"
                     /translation="MNLYNIIFTGSEQALGAAQAMLAEAIEKNGKEHKVAFPDTAYSL
                     PCIYAATGQKMNTLGDLEGALEVVKSLINRTHLLEHAFNAGLATALAAEVIEALKYST
                     MDAPYSEPCAGHITDPIIRSLGVPLVTGDIPGVAVVLGECPDAESAAKVIKDYQSKGL
                     LTFLVGKVIDQAIEAGVKMGLELRVIPLGYDVTSVIHVVSVAVRAALIFGGLTPGDLN
                     GLLEYTANRVPAFVNAFGPLSELVVSAGAGAIALGFPVITDQTVLEVPMNLLTQKDYD
                     KIVATSLEARGIKIKVTEIPIPVSFAAAFEGERIRKSDMFAEFGGNRTEAWELVVKKE
                     ATEVEDHKIEIIGPNIDEVDADGVLRLPLAVIVKIAGKNMQEDFEPVLERRFHYFLNY
                     IEGVMHVGQRDMAWVRISKDAFDKGFRLEHIGEVLYAKMLDEFESVVDKCEITIITDA
                     EKVSELKGEAIAKYNARDERLASLVDESVDTFYSCNLCQSFAPAHVCVVTPERLGLCG
                     AVSWLDAKATKELDPTGPCQPIEKGECLDDRTGVWNSVNETVNQISQGAVESVTLYSI
                     LEDPMTSCGCFECICGIMPEANGFVVVNREFASVTPVGMTFGELASMTGGGVQTPGFM
                     GHGRHFISSKKFAYAEGGPERIVWMPKELKDYVADKLNATVKEMTGIENFCDMVCDET
                     IADDSEGVLAFLEEKGHPALAMESVM"
     gene            22802..23179
                     /gene="gcvH"
                     /locus_tag="KR250_001466"
     CDS             22802..23179
                     /gene="gcvH"
                     /locus_tag="KR250_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HBH0067413.1"
                     /translation="MKLLPELKYSKDHEWVKVIDGDVVYIGITDYAQDQLGEILFVET
                     PEVEDTVTKGVDFGVVESSKVASDLISPVNGEVLEVNEKLEDEPECINEDPYENWILK
                     VKLADVAELDTLLSDKEYEAGLE"
     gene            23291..25219
                     /locus_tag="KR250_001467"
     CDS             23291..25219
                     /locus_tag="KR250_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4445 domain-containing protein"
                     /protein_id="HBH0067414.1"
                     /translation="MIKVSFTPNNKEVYCNEGDILLEVARNADIFIDAPCNGNVSCGK
                     CKVKLLNGKVDTEKTRHITDDEWEQGYILACCTKVISDIEIEVPSKVSSSMHGMKIEG
                     SNKKEDREIFERAKKIIEEHNLQFKTNIKKKYIEMEEPNLDDNISDVDRLERYVRNNL
                     GYNEIDFRLDILRKMPTVFRKSDFKVTITYVQKQKKLTIINIEQGNKENSLYGVAIDI
                     GTTSVVVCLVDLYSKEVVDKASSGNAQIKYGADVINRIIYSTKKNGLETLHKAIVEET
                     INPLLKTIYERNGIDKEDVVTLVAAGNTTMTSLFLGVYTDFLRQEPYIPPFLKSPKLM
                     GENVGLFVNDSAYVYLAPSVASYVGGDITAGVLSAGIWSSEENVLFIDLGTNGEIVFG
                     NKDYMMSCACSAGPAFEGGGISCGMRASAGAIEKVIIDKETLEPTLKIIDECAPVGIC
                     GSGIIDLICQMITKGVIDRRGKIYRDLDNKRVRFNEHEIGEYVLAFKEEFDLENDIVV
                     NEVDIDNFIRAKGAIYSGAYTLVDSLGMDFSILDRVYIAGGIGNNLDIENSIIIGLLP
                     DIDREKFTYIGNSSLVGSYLALISKDAKNKLEEIGNQITYVELSVYPSYMDEFISACF
                     LPHTNIEQFPTAKKLLEE"
     gene            25244..26116
                     /locus_tag="KR250_001468"
     CDS             25244..26116
                     /locus_tag="KR250_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBH0067415.1"
                     /translation="MNKNLSEKMEIAFDIKLKNFGNKIEFAYPNQTLALSTTSNQCSL
                     KCAHCNGHYLNNMVPIEEYEEKVQSRNITSFLLSGGCSYEGDVPINTHINTIKNLKEQ
                     GYRLNAHLGLMDKDSIVELCKYLDIVSFDLVFDDETIREVYKMKKSKEDYIEVYNTIQ
                     EHTEVAPHICIGLKGGQIKGEYEIIEYLQKNPPNKLTFIVLIPTKGTEYENVEPPELE
                     GVADILCEARINLPDTEINLGCMRPRGVYRKELDQLSIMCGVNRIVLPSRSAKNKAIE
                     MNMTINECKECCVL"
     gene            26113..27051
                     /locus_tag="KR250_001469"
     CDS             26113..27051
                     /locus_tag="KR250_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBH0067416.1"
                     /translation="MIRLSVGTAIELGILNKKSDIPPTTAYIMIGEKCINKCSFCSQS
                     IESSTRKDKLSRVIWPEFSKEEILDALKAYKGKNIKRICIQSMASEEAHNSVLDFINY
                     ISGKIDMPISLSAKLENDEQIKKFFSVGVDKIGIAIDAANKELYEKIKGNNYDEKLKF
                     ITEMSKSYPNKISTHIIVGMGESHEDIYNLYTYLKENDVTISLFAFTPVRGTKMEKIN
                     QPSIESYRRVQLMSYMINKGYPKEYFKFKNGYLNSIKLDNDILKDINKGYPFEIRGCK
                     DCNRPYYNERPGSTIYNYSRPLNQSEIDLAIREINL"
     gene            27098..27913
                     /locus_tag="KR250_001470"
     CDS             27098..27913
                     /locus_tag="KR250_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HBH0067417.1"
                     /translation="MNQWRVIHNKSYEGAMNMAIDEAIFTAYKKGHNKPTLRFYTWEP
                     ACLSIGYFQKLEDEIDLDKCRCMNIDYTRRITGGRAVLHDNELTYSIIIGEDNPLIDK
                     SINLSYRYISEGLVKGLNLSGIETDNLNRGERISRENLSAACFNAHASYEVTINNKKV
                     IGSAQSRKDGVLLQHGSIILDFDVEKLFKLIKTKTPELKERAMKFTAKKASGIENEIG
                     RKIDIDILQKNIVKGLAEQFNVEFVEGDLTDYEKQLVKELYEKYKNEEYNKKR"
     gene            28064..28255
                     /locus_tag="KR250_001471"
     CDS             28064..28255
                     /locus_tag="KR250_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CooT family nickel-binding protein"
                     /protein_id="HBH0067418.1"
                     /translation="MCESSAFICKSNNELEKVMENVVNIDPCDGKIYLTDLLGEQKII
                     DGIIKEIRLMDHKIIIQEN"
     gene            28274..28690
                     /locus_tag="KR250_001472"
     CDS             28274..28690
                     /locus_tag="KR250_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3842 family protein"
                     /protein_id="HBH0067419.1"
                     /translation="MIIAVIDGMGGGIGAQIVSSLREELPTYVEIYALGTNSIATSSM
                     MKAHANKGATGENAIVVSAKKANIIVAPISVIIPNSMMGEVTCNISEAIADSEALKIL
                     LPIMPENVELVGLEGKPLALLVKDSVNLIKKEFNIK"
     gene            28706..29056
                     /locus_tag="KR250_001473"
     CDS             28706..29056
                     /locus_tag="KR250_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter"
                     /protein_id="HBH0067420.1"
                     /translation="MIFDKEIVRYHHGHHDHDHCHEHTHGDICHEHPHDHAHDHDHEH
                     SHEESSESKDEKTLKILLVHWINHNETHEEGFREWVEKARAIGKEETAKSIEKAIEYM
                     EEANKMLLEAKKHM"
     gene            29602..30381
                     /gene="hisJ"
                     /locus_tag="KR250_001474"
     CDS             29602..30381
                     /gene="hisJ"
                     /locus_tag="KR250_001474"
                     /EC_number="3.1.3.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ"
                     /protein_id="HBH0067421.1"
                     /translation="MKDGHIHSPYCPHGSKDDFEKYVQRAMDVGITEMTFTEHFPYAN
                     GFRDPAPENDSCMSIEDLPKYFNDVKKLKEKYEGKIKINVGSEVDFIEGYEEGIKENL
                     NKYGKELEDSLLSVHIIKIDDDYYCVDYSVEEFQNLIDKLGSIEAVYNKYYETLIKAV
                     NSDLGIHKPKRIGHLNLVRKFNQVFPYDYKGNTILEELIKLIKEKGYELDYNVSGNRK
                     EYCKEPYIDGYLLELVKKYDIPLILGSDSHCAEEINKYDIV"
     gene            complement(30694..31575)
                     /locus_tag="KR250_001475"
     CDS             complement(30694..31575)
                     /locus_tag="KR250_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4300 family protein"
                     /protein_id="HBH0067422.1"
                     /translation="MKKRLIIMILSVVLVLSSILTIFAYSNIKYNNNKLIYSNMIDKK
                     TQNSVKEILKENKINEKDIDTFIKAVNNYNKLQVKILQNNINISKSGYSSINAKQVPY
                     NLEKLQDNWVKKFPDYMDVNCRITAFRLFKDFINSNKKFTGDSIDLNVDLDTIMNNKD
                     AKFSIKDVEKFINFFSAIPAKDTDDTIKISEQIKNEWKKRKISFKNNKNISIINGFLR
                     YPETKNVFIGHTGICIKTKNGILFLEKYGVTSPYQVTKFKNKEDVKNYMFNRLKMSEG
                     EIELPDPIIMENDKLMK"
     gene            32128..32640
                     /locus_tag="KR250_001476"
     CDS             32128..32640
                     /locus_tag="KR250_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0067423.1"
                     /translation="MKVKKFKSGIVMALTVMAIGASLTNVSAMELNKSKEFETKYNEM
                     KMDFQEEYGKEDKFNITLDDEFTKEEVNGAKGIIKTTNKMNNEVVVLNYFDEMKSGIE
                     FELKPKAEKNAILRDEFQQGYGKEDKFKVVLDDEFTKEEVNGAKGIIKTTDKETGEVI
                     VYNYFDELEK"
     gene            complement(33093..34190)
                     /locus_tag="KR250_001477"
     CDS             complement(33093..34190)
                     /locus_tag="KR250_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HBH0067424.1"
                     /translation="MKKTYLFSPGPVMVTDKVRNSLLHYDICHRGNEFMDLFKDTQQK
                     INKLYNATSNYYSLIVSGSGTSVNECVLSSIFDKEDAVLLVSNGVFGERLEEILSTYN
                     VKTYKPNFEWAEYPDLDILEKYIIENSDIKVIAMVFHETSSGMINPVPELGKLAKKYN
                     KIFFVDAISASAGEYIDVEEFNIDIITGVGGKALGAFPGSAYLCAKESILQNIKENQC
                     KNVYLNLYKHYKVAKSSSQTPNTPNVTLIFALNEALTEFLEDDSKIERYKECSAILRN
                     GMEELGLTFLLPDKYMSNTVTSVFLPKEINVNEFILGLERESGYVVYPGKGKFLDANI
                     FQVANMGEIYPEDCYKFLDILKSKLEIKKVI"
     gene            34483..36009
                     /gene="glpK"
                     /locus_tag="KR250_001478"
     CDS             34483..36009
                     /gene="glpK"
                     /locus_tag="KR250_001478"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HBH0067425.1"
                     /translation="MKYVLAIDQGTTGTRAVLINEKSEFVFSDYMEHTQIYPQSGYVE
                     HDPIEIWNNTKTVSNNVLQQAFENGINESDIKGIGIDNQGETVMLWDKNTGKPLYNAI
                     VWQCRRTYDYVENLKNIPGLENKIIDKTGLIIDPYFSGTKIKWVIDNVKGVKEKIKNG
                     EVLAGTLDSWLIWKMTGGKSFLTDVSTAARTMLFNIRDMDWDRELLDIIGVPRSILAD
                     IMPTSGDFGTTDKEAFCGVSIKITASMVDQPAALFGNGCFEPGMVKNTYGTGCFLYMN
                     IGEELNIAGNGILTTVAWKIGDKVTYAYDGGVYIAGAAIQWLRDGIGVIKNYSETDDM
                     ANSISSTGGVYFVPAFAGIAAPYWDQYARGTMVGITGSTKKEHIVRATLESVALQVKD
                     VVDSMNLASGKKIKMLRVDGGITKNNFTMQFQSDILGIPIEIAENPETTVLGVGYMAG
                     LSCGVWNSIDEIRSEFTISKKYIPNMTTQKREEILLGWNQAVKRAMNWENDTKKVCAN
                     "
     gene            36050..37165
                     /locus_tag="KR250_001479"
     CDS             36050..37165
                     /locus_tag="KR250_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HBH0067426.1"
                     /translation="MPFDKIILYIMAVGVLLGALDRIIGNKFGLGEQFEEGFNSMGPL
                     ALGMVGIVCLAPVISDVLGPIIIPIFNLCGADPSMFATILANDMGGYPLAMELAHNKE
                     AGMLAGLVVSSMLGCTLVFSIPVGLGLIEYEDRPFFAKGLLIGLITIPFGGIIGGLIA
                     GFNLSMVIINMIPVIILAVILAVGLKFNSTLMINGSLVFGKFISILITIGLGAAAFQE
                     ITGIVLIKGMTPIREGIQVIGSIAIVLLGTFPILHLLVKVLNKPLTIVGGKLGMDATS
                     AAGLVFTLANSIPVYKMMKDMSPKGKVVNTAWLVCATAALGDHLGFTAGVVPEMITPV
                     VIGKLVGGVLAIALAILMTKDLTLEEEQSIKMKKDAV"
     gene            complement(37446..38210)
                     /locus_tag="KR250_001480"
     CDS             complement(37446..38210)
                     /locus_tag="KR250_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBH0067427.1"
                     /translation="MLPQERYEKILSILKSDNIIKINDIVQMFDISIETARRDLAHME
                     SIGLLKRVYGGAIPVIASSVELNYVKRKKINSNDKKQIAIKCSEFINTNDTIFMDTGT
                     TVLEVSKNLKDKKNLTVITNSILVANELMNTNIKVYVLGGYLRETEGSFSGPLTLSAI
                     EQFNVDKAILGAGGITLENGISDYHLEEALVRKKMIERSRKSIVVVEPPKFGINSFAS
                     VAPIEHIDTIITSASIDHNIIIEFSQKGIPLVIADN"
     gene            38469..38559
                     /locus_tag="KR250_001481"
     tRNA            38469..38559
                     /locus_tag="KR250_001481"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:38503..38505,aa:Ser,seq:gct)
     gene            38647..39435
                     /locus_tag="KR250_001482"
     CDS             38647..39435
                     /locus_tag="KR250_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein"
                     /protein_id="HBH0067428.1"
                     /translation="MTTLIFFIVGIASLVVAFVIDGGHVGALFVFSSALVVFGGTIGA
                     VGASTPFPVFKKSIKIMLLTFKNKKPNNIENIIYFKEIATKARKEGLLSIDKEISEND
                     ELDPFIRKGLELMVDGVDAVTIRNILESQIQITSKRHKEGIAIFDSAGGYAPTLGIIG
                     TVMGLVHILGNLSSNPDALGPQISVAFLATLYGVGSANLIWLPIASKLKAINQQEVSE
                     RLLVIEAILSILEGDNSNLMTEKLKIFLNSKELIELEEIGGRAE"
     gene            39456..40196
                     /locus_tag="KR250_001483"
     CDS             39456..40196
                     /locus_tag="KR250_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HBH0067429.1"
                     /translation="MLYDEDEKEENNERWLLTYSDLITLLMIFFVIMYSMSNVDAEKY
                     KQLSQSLNSAFGGSSGVIEGGNSKIEPVVEPGSNDLDTLQNAKFKKIGEEIQKYLNEN
                     GMANSVSLRVQDRGLVISLKDTILFDTGKAIVKDNSRDKIIQIGKMLNEMNSYMRVEG
                     HTDNMSIKNSEFKSNWDLSVMRATNVVQLLIDNAGIAPDKLSAVGYGEFRPIAENSSE
                     EGRSKNRRVDIVLVDSKYDNVENVSKDK"
     gene            40372..40746
                     /locus_tag="KR250_001484"
     CDS             40372..40746
                     /locus_tag="KR250_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0067430.1"
                     /translation="MGNKNKNTKYKDKTRERYNKKVENLNKEKLNKINKQSKKVDKID
                     DIINKSSIVLCVLLVVFSLLYLFKLVQLNIIMILLVITVAIQQGLVSFGAFRNKNYVK
                     ALFSIFVIVCFLIAFKYVVNPV"
     gene            40838..42298
                     /locus_tag="KR250_001485"
     CDS             40838..42298
                     /locus_tag="KR250_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBH0067431.1"
                     /translation="MNRCKFAKLIITSMIVLLLTGCSLKKEDNYKLDPENPTSIQIWH
                     YYNGQQKMAFDKLVKEFNDTVGAEKGIIVEAFSQGNVVDLSEKVLDSANKKVGSEDVP
                     NIFAAYSDTAYEMDKLGLLVDMDKYISKEELNEYIDEYVEEGRINSKDEFKLFPIAKS
                     TEILMLNKTDWDKFAKATNSNINDIKTIEGLVETSKKYYEWTDSLTKEPNDGKAFFGR
                     DALANYIIIGSKQLGVEIFSVKDGKVTLNIDEKVMKKLWDNFYVPYINGYFASYGKFR
                     SDDAKTGDILALVGSSSGAAYFPDRVMLNDNESYNIETSVFQAPRFKGEGKYAVQQGA
                     GMAVTKSTKEEEYASVEFLKWFTDKEPNIKFSIASGYLPVKKKTNDLDVIKEVMKKDN
                     LSLSDKVEGSIASSIEQINSCNLYTNKAFDGGTKARQILEYSMLDKAKEDSNKINELV
                     KSGTSKKDAVAQFNNDKNFKTWFESFKAELENAIKK"
     gene            42351..45380
                     /locus_tag="KR250_001486"
     CDS             42351..45380
                     /locus_tag="KR250_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBH0067432.1"
                     /translation="MENNLRKNNSIMKKMIFPLIIVMLIQTSLFCATILWGGTIKKLN
                     DNSFDILNERVINRKNYIQNEMLQRWSNTSETEATINSSVKKVLNEKNALIEDVGNNE
                     EINKDIIKSISNDLIYLLRKNSVTGAFIILSDKNINDKSIDSKGEISRTGLYIRDLDP
                     KFNPNDNSDLLIERGSSDIARSFGISMDSYWEPKFSFKSENEQDGDDFFYKPFRAAID
                     NPNVSSSNLGYWGSKFLLTNAGDRLMTYTVPLIYEDGTVYGVLGIDLSVNYLRELLPY
                     SEINGNKKGNYILGVDRNNDMAFENVISNGYSLKEKFWDGLHSNLEQKESYKNIYKIK
                     NKDNECGNGNIYASVQYLDLYNSNTPFENERWALLGIIEEKSLLSFSKRVEKLVIISI
                     AISLLIGLIGIYIASRLFTKPIVLLANKVRNSNPSKPVNLDKINISEIDELSSAIELL
                     SANVADSSSKLSQIIEMVDMPIGAFELYKNAENVFCTKEFFSVFTTEIKFEEYGYMEK
                     NKFLKKLRGLDKYLQESYETENTYIYKFESKDKKLKWIRMNIFEDELKVLGVVVDVTK
                     EFIEKRKIEYERDFDILTNLLNRRAFHSQIQEKFKNKDELKIAAFIMWDLDNLKYIND
                     TYGHDAGDEYIRCAADILKKFISWNGIVSRLSGDEFYVFIYGYESKDSIRDIINSVKK
                     QMNNTLLKLHDGTEFKIRASAGISWYPDDSTNYDELMRYADFAMYQIKNTIKGEVSEF
                     DINIYNKDSFLLSNREELNKLIDGQLVEYAFQPIVDCKGKVFAYEALMRPKIKTFSSP
                     IDVIRLARSQSKLYQIERITWFKALKSFKKYKEKFGDCKLFLNSIPNNNLSKVDFEEI
                     EDLYGEYLNRVVLEITENDKINEDFIEKKKAYIDHWNIEIALDDFGTGYNGDAVLLYI
                     MPDYVKIDMSIVRGIDKDENRQKILKNLISYSNERNIKVIAEGVETKGEMETLINLGV
                     DYMQGYYISKPSFIPEDISDKIKKELKICHENRNDVV"
     gene            45457..47523
                     /locus_tag="KR250_001487"
     CDS             45457..47523
                     /locus_tag="KR250_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HBH0067433.1"
                     /translation="MVELIITTNIDIRKLNENQLEAVEHIDGPCMILAGPGSGKTRVI
                     TYRIANMVVNKNIAPTRILAISFTKASSIEMKNRALNLSDDIRLNKVTYGTFHSVFFK
                     ILRYFERYNLDSIFDEKSKRMTIKAILKSLNIENADDDENIGQVINEISYVKNELMDK
                     NEFNSEVLTKDEFLKVYNLYEEQKSKVNKIDFDDMLIRTYYLLLNNKSALEMVRNVYK
                     YILIDEFQDINKVQFEVLKLICSPLNNIFAVGDEDQSIYGFRGARPDFLLEFEQYFNN
                     TKKIILDINYRSKSEIVHTANRLIDKNKNRYEKIIKCSQGDGGSVTYISPHDSEEEAL
                     YIAREIIDEIQKDYVEYSDFAVIYRTNIQSRALVDVFMDMRIPFVVKDSVITIYDHWA
                     SQDILAYLRIGINPKSNKDWLRIINKPFRYISKDSINMVKDEKDFITALINKCNLHPK
                     QVKTINDLEIDLSYLNTLNPKNAISYIRTSLDYDRYVLDYCSNRKIKTNGLVEILNEL
                     ESSATNFNTVTEFLEHIDRVKTELTENNKNKQTEGVIFTTMHSAKGLEFRNVYIIGVN
                     EGTIPHEKSYDICKEEKKEEQLEEERRLMYVAITRAEEKLCISSTLNKYGKKVDKSLF
                     INDIKSPTKKEIDSIGVGDKVYHKKFHEGEIIKKDGIMFTIKFKDRERILDLKTCLLK
                     NIIYTI"
     misc_binding    47906..48116
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            48342..49157
                     /locus_tag="KR250_001488"
     CDS             48342..49157
                     /locus_tag="KR250_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBH0067434.1"
                     /translation="MKVFKKLLSLGLVLGLTLSLVGCSGGGEKTKLEQIKDNGKLVVG
                     TSAEFPPFEFHKVVDGKDSIKGFDIMLAEEFAKELGVKVEIKDMSFDGLIGALNADQV
                     DIVLAGMSPTPEREKSVDFSELYYLSRNAVIVKDADIDKVKTEDDLKKLRVGVQAGSI
                     QEEYVVNTLKMTTTKSLKAIPDLITELKNGNIDAVVTNEAVSLINVKKYDGIKMANTE
                     VGKDVTEGMAAAIKKSDNNKDFIELLNKKIKELQDGKKIEEFLNEASTEAASN"
     gene            49185..49862
                     /locus_tag="KR250_001489"
     CDS             49185..49862
                     /locus_tag="KR250_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBH0067435.1"
                     /translation="MSLDFSFLSRFGTSFLEGTGVTVSISLVALCFGFIIGIIICMAK
                     ISKSKVLRAISSIYIEVLRGTPLLVQIYIVWFGLPQLGIRFPMLFGIPSEFIASAFAL
                     SVNSGAYVAEILRSGIQSVDNGQMEASRSLGLNYWSTMRYIIIPQAIKNILPSLANEF
                     ITLVKESSIISVIGVVEIMRTADIVKNAAFRALEPLIVAAAIYFVITFTLSRLVGLLE
                     KKLSVSN"
     gene            49921..50643
                     /locus_tag="KR250_001490"
     CDS             49921..50643
                     /locus_tag="KR250_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004398740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBH0067436.1"
                     /translation="MITIKNLSKSFGDLNVLKNIDLEIAKGEIMVIVGPSGSGKSTLL
                     RCMNLLEIPTGGEIIFEGKNLVDKKTNIDEVRQNIGMVFQNFNLFPHKTILDNITLAP
                     IKLKKMTKEEAEKKAEILLSRVGLLDKKDSYPSQLSGGQKQRIAIARALAMEPDMMLF
                     DEPTSALDPEMVNEVLDVIKELAKEGMTMAIVTHEMGFAKEVADRVIFIDGGSILEDN
                     TPEEVFGNPKHERTKAFLAKVL"
BASE COUNT        19764 a         5598 c         9649 g        15878 t
ORIGIN      
        1 taggttatta gagtgtgcta tttttttcta gataatctaa aaaatctttt tccttagtgt
       61 cagttaaaaa acataattcc tttactatat tattcctaag gtcaatgaaa tcactaattt
      121 gagtattttc attagtgtat tcaagagact ttataataag tagtaaatct ctttttgata
      181 aagagttgat agatactttt tcaattaatt ccataaaaaa cttcctttcc tcacattact
      241 tgaaattaat aaattaatta tatagatatt atttagactt tacatatact attttaacct
      301 ataaggggta caaaaagaca gtataatctt aaaaagaggt gagaaatatt tgaaaatttt
      361 attaacaaca ttaaattcca agtttattca tacaaatttg gctataagat atctaaaaga
      421 atttgtaaga gatttgatag aagttaatat gaaagaatac acgataaaca acgatttgga
      481 ctatatttta aaggatatat ataaaaatga atatgatata atattattct caacatatat
      541 ctggaatgtg ggagatatag ttaaactttg tgataatcta aaaaaaatta gacctaatac
      601 taaaatagct ttgggcggac cggaagtgag ttatgatagt tatgaggcaa tgaaaaagta
      661 tgattttgtg gattatatac tttatggaga gggagaatta atttttagag atttagtact
      721 tcacttgcaa ggaaagatgg aaataaatga tgtaaatgga cttgtatata gacaaggcaa
      781 tgagataata gtgaataagc ctatggaatt acttcaagat ttggatgaga ttcctagtcc
      841 ttatgaaaac ttgaatccaa aagaatatga aaatagaata gtgtattatg aatcatcaag
      901 aggttgccct tttaactgcc aatactgttt atcatctact attccaggtc taaggtattt
      961 tagtttagac aggataaaga gtgatttaaa agctttaata gatgcaagag tatcacaaat
     1021 aaagtttata gataggacat tcaatgcaaa tagaaaagtg gctatggaaa tcatggattt
     1081 tcttatgaaa aatgataatg gatatacaac ttatcatttt gaagttacag cgtatttgat
     1141 agatgataag atgcttgagt ttttggctga ttgtaaagaa ggtctttttc aatttgaaat
     1201 aggggttcag tcaacgaatg aaaaaacact tgatgcagta ggtagaaggg atgattttaa
     1261 aaagttgtct catgttgtac aaactgtagc atcttataga aatatacatc aacacttaga
     1321 cttaattgca ggactgcctt atgaagatta taagagcttt gaaaattctt ttaatgatgt
     1381 atttaatcta ggtatagagc atctgcaatt aggcttttta aagatgatta aagggacagg
     1441 tatgagaaaa gttgcagatg agcatggttt taaatataag gattatgctc catatgagtt
     1501 tttatacaat aattatataa gctatgaaga aactttgaag ctaaaggata tagaagatat
     1561 actggagaga tactacaatt ctaaaaactt tgtgttatct atgagatata ttataggcag
     1621 attttataaa aaaagccctt ttaaattctt tgaggttttt gccaagtatt ttgatgaaaa
     1681 tggctacttt gatttggcac aaggaaagaa tcagttatat aaaatattga tggatttcta
     1741 taatgaaaaa atcaacatag ataatgatgt attcaacgat atattaaaat atgactatat
     1801 atcactgggt aaaacatcaa atataccaca gttttttaat aagctagatg tggatgattt
     1861 taagaataga tgccatgtat ttttacaaga taatgataac ttatcaacat atttacctag
     1921 ttttgttgat aaaccagcta aacatataat aaaatatgtt cattttgagc catttagatt
     1981 taatgttgtg gatttaaaaa atgatataaa tacagaaata agagaagtag aaaatgtagt
     2041 cttatttgag tatgatgata aaaaaatatt tgagaaatct aaaactcata aagtagagat
     2101 ttaagtattt aggaggataa aatggatttt aagatagaaa aatatttgct aaaaaaagca
     2161 gaagaattag cttttataac tataaaacat ggtggagagt ttaaattaaa aagctatgaa
     2221 gtgccaaaag gtggattaga tgtacctata aaaaatgagg tattagtaaa agggataaaa
     2281 gaaaagactg ctcaagataa gttaaattca atgtctatag cagatgcaat gatatatata
     2341 attggaatag atagtaagtt taaaaacaat gcagagtatg agaagttttt aaatgcttta
     2401 tcaaaagata ttgatttgga tttgaaatcg tatatgggat atatgtcgag aaaatacttt
     2461 gagattggtg aacatacaga ttctttaata tatataaaag catttataac aatgtatcca
     2521 gatgatttag atgcaatgta taactatgcg attgtttgcc aagagatagc taaacagtat
     2581 caaaaagata tggatgataa ggcaatgaat gcattcttac ttgaagccat ggctaagctt
     2641 gaaaaggtaa ttgatgttga tgagaatttt gcattaggtt attatcactt aggctaccat
     2701 tattataatc aaggtcaata tttaaagact aagctgactt gggaagaagc gttaaggcta
     2761 gggttagatg ctgatttagt agctgaggta caagagaatc tgggcaagat ggactttaaa
     2821 gttcaatatg aagaaggata tacacttgta ttccaaggta agtttaagga aggattagag
     2881 aagttacttc cattagaaga agaacatatg gattggtgga acttgctgtt tatgatagca
     2941 cttggatata agggtatggg cgaaatagag caggctaaga tgtatcttga gaagatattg
     3001 ataataaagc ctaatcaggt ggatactata gtggaattgg ggctttgtga ggcttataag
     3061 aataatttag ataaagcaat agagtatttt gaacaagcgg ctaagataaa ggaagaccca
     3121 gaaatacttt gcaatttagg aatggcttat ttaaataatg gtgatattga tgatgcaact
     3181 tactatatag aaagagctta tgaattaaat ccacaagatg aaattacgat tgcttgtcta
     3241 agagaacttg gaataaataa gtaaggatat tgttaagttg tatttaattt tattggttaa
     3301 gtggggctat ctcaaaatag agataagcct cttttttagt gctgaaatga tgaacatata
     3361 ttttactttg tataaaaaag agtgccttat acagtgcaaa gttcattttg agacactctc
     3421 actttttatt atgatttatt tttctttagg tgtatccaac ttagatgtat ccaacatgat
     3481 agaagaaaaa tctttttgta tttgaagtaa aaatttttct gctgcctttg aaaatatttg
     3541 atgttttttc catataatat ttaatttagc ttctaaagga ggagatagag gtctaaaaca
     3601 aagattactg ttttctgtag tattgacgag tctatctaaa cacagagcgt agcccagacc
     3661 ttcttctacc aaaagagaag cgttataaac taaattgtat gttgcaataa tgtttagttt
     3721 ttcattactg ccttgaagcc atttagaaat tgctttatca acaagtgtct gtcttgaaat
     3781 aataagaggt atattgcata aatcttttgg atttattgtg ctttgtgatg caagggcact
     3841 atctttacgc attagtaaac cccatacatc agtagcagga atttgtgcat aatcatactt
     3901 ctgtatattt gcaggttcaa ttaaaatacc aaaatcaagc agtcccttgt ctaatttttc
     3961 agtcacatca tcagcattcc cactaaaaag gtgatatctt acattggggt atttttgatg
     4021 aaaatgatgg gcagttctag ctataagtcg catagcttca gtttcaccac ttcctatata
     4081 tatatcacca ctaataatct cattgggatt gtcaaattct aattcaacct tatcaactaa
     4141 tgatataatt tcctctgctc ttttatgtaa aagcctacct tcatttgtta aggtgatttt
     4201 tctatttcct cgaaccaata atttaactcc caactcttct tcaagttcca taatttgtct
     4261 agaaagtgtt ggttgtgtta tatgtaaaaa ggttgccgca ccagatatac tttcttctct
     4321 tgcaattgct aaaaaatatc ttaatactct tatttccata attatccctc ctcaatatta
     4381 acttatttat tatataatta tatcaattta tatgcttttt aagtatagtt aagtatttaa
     4441 tatagatatt tgatatagat ataattgtat attacactaa aagaagcaat gatattttaa
     4501 taaaaataaa ttaaattaat ttttattaaa atattattta gaatgtacaa ataaattaaa
     4561 gcacatattg aaaggatgta taaaatgaat atcaatgaat ttttaacaca tatcaatcaa
     4621 agaaaacctg tttctagtaa atcagaaata catgaattta tggttaagtt gagtaatgag
     4681 gcaatgaaaa tcacaagtaa gttaaattct tcttatcatg aaccagaaga agtccgtagt
     4741 ttattttctg agttaattgg aaaaaaggta gatgaatcat tttttatgtt tccaccattt
     4801 tatacagatt gtggaaaaaa catcacaata ggaaaaaatg tttttataaa tagcagttgc
     4861 cattttcaag accagggggg gattgagatt ggagataata cgcaaattgg gcataatgtg
     4921 gtattggcta cacttaacca tggtattgca ccagagaaac gtggtacaac ttatccttct
     4981 ccaatcacaa taggaaaaaa tgtatggatt ggtgctaatg taactgtagt tccaggagtt
     5041 actattggag ataatgcaat tatcgctgct ggagcagttg taacaaaaaa tgttgcagaa
     5101 aatactattg taggtggtgt tcctgcaaag cttttaagag ctattgatga aatcccagaa
     5161 taaaatatta aactgtcttt tagttatata aattctttat aacataaaaa ataaattgag
     5221 aaaaggtata gcaaatatgg tattatttag ttaaatatat ataagggagg ggttactttt
     5281 gataaaaaaa ttagatgata ttttagaaca acttaaggga ggagagaaaa ttgtactctc
     5341 tgtagcagca gctcatgata aagaagtttt aatggcaata aaggatgctg tagaaagaaa
     5401 tataattact cctatattgg ttggaaatga aggcaaaata agagaaatat caaaagaaat
     5461 aggatttgat ttaagtggta tcaaaatagt ggataaggat gatatcaaag agtgtgcaga
     5521 aatagcagtt aaattagtat caagtaaaga agctgacttt gtaatgaaag gattattaga
     5581 tacatctgtc atattaaaag aagtgttaaa taaagactat ggtcttagaa ctgatagttt
     5641 attaagtcat gttatggtat atgagttaga aaaataccat aaattactta taactacaga
     5701 tggaggtatg aatatagctc ctgattatga acaaaaggca aagatattaa aaaattctat
     5761 aaaagctgca aaagctttag gaatggaaac tgtaaaagtt gcatgtttgg cagctaaaga
     5821 gaaagtaaat cctaagatgc aagcaactgt agatgcagat atgttagcaa aagcttgtaa
     5881 agaaggagaa tttggtgaaa atgtaattgt agaaggtcct ctggcttttg atttagctgt
     5941 ttcaaaagaa gctagtgaga taaaagggtt taaaagtgaa gtatctggag atgtagatat
     6001 catattggtt ccaacaatag aggttggtaa tggaataggg aaagccttta catatatggc
     6061 agactctaaa tcagcaggta taattatggg tgcaaaagct cctatagttt tagtgtcaag
     6121 agctgattca catgaatcta aattatattc tattgcatat ggggctattg tagctaaaaa
     6181 tatgaaatag gataaatcgc ttgaaataaa tgaattaaag tagaaaaaat agtcaaaatg
     6241 tctaaattaa tgctgtatta aaaaagtttt atataaaaaa taattatagc ttcaaatgaa
     6301 tatatacaaa tgttcaattt gaagctataa ttaatattaa agtaggaaaa ataaaaaaat
     6361 tcaaaaaaat tagtccaaaa aatgctattc taagaaattt ttactaatta agtttaggga
     6421 gtaaaaatct gtaataagtc caaacagtag tgaaagataa taaatacaca aacaatctgc
     6481 aataatataa aaatgactag aaaaatttca aatttgattt aaaaaaatca aaacataatt
     6541 taattaaact gaagtatcaa aaaagtaggt ttagatatta acaaatagcc aaaaacaagc
     6601 gataaaatca acttaaatta agtgtagaaa ataaaatttt tatgtattat ctaattttgt
     6661 taaaaaaata tttaaatcta aagcaaagca ctgaaaatta gtatataatc aacttagaaa
     6721 gcatttattt tattatttta tgacatgaag tcagtcacct gggggatttt aaaaatcaaa
     6781 aaaaggggat aggaatatgg atgaaaaaat gttaacaatt gatttaaaca gccagaagat
     6841 gatagctaaa gctagagaag aaggcgtaga aacaatgtat gatagaaaag aaggctttaa
     6901 ggcacaatgt ggttttggtc ttcaaggtgt ttgctgtaga atatgtggaa tgggaccatg
     6961 tagaataagc cctaagactc caagggggtt atgtggagca gatgagcata ctatagtagg
     7021 tagaaacttt gcaagaatgg ttgctggtgg gacagctgcc cattcagacc atgctagaga
     7081 tatagcacat acattagcat tagctgatcc aaatggaaac tataaaataa gagacgaagc
     7141 taagttaatt actttagcta aagaatggga tgtagagaca gaaggtagag atatctatga
     7201 tgtagcacat gaagtggcag aaattgcatt aatggaattt ggaaaacctt ttgggacatt
     7261 aagatttata aaaaatgcac cagaaccaag acaaaaaata tggaaagaat acgctataga
     7321 gccaagagct atagatagag aaattgcaac aataatgcac tctactcata taggatgtac
     7381 tggtgatata gatagtttaa tacacatgag tcttagaact tctatggctg atggttgggg
     7441 tggttctatg ataggtactc gtctaagcga tatcttattt ggaactccag tgccaagaag
     7501 aacagaggcg aatttagcag tattagaaga aaataaagtt aacataatat tacatggaca
     7561 tgagccagca ttatcagaaa tgatagtttt agcatcagaa gagccagact tagtagcttt
     7621 agctaaagaa gttggtgctg atggaataaa cttagcaggt atgtgttgta caggtaatga
     7681 gattacaatg agacatggtg taaaaatagc aggagacttc catcaacaag aacttgcaat
     7741 aataactgga gcagttgaag ctgtaatagt tgacgttcaa tgtatattcc ctgcattagc
     7801 tagagttgca gattgttacc atactaaatt tgtaacaact tctccaaaag ctaagataac
     7861 aggttcaact tatattgaat ttagagaaga acaagcactt gatgatgcta aagcaatagt
     7921 taaagaagct attttaaact ttaaaaatag agataaatca aaagtactta tacctgaatt
     7981 aaaatcaggc gcaacagttg ggtatagtgt agaagcagta gtaaatcaat tagacagagt
     8041 tgtaaactct catatagacc cagcaggaac agttaagcca ttaacagact gtttaaaagc
     8101 tggagtatta agaggagcag ctggtgtagt tggatgtaac aatgctaaag gagtttcaaa
     8161 tgaggctcac gtaacaataa tgaaagaatt aatcaaaaac gacataatag tagttactac
     8221 aggttgtgga gcttcagcag cagctaagtt tggtttaatg gaatctgatg cggctgaaaa
     8281 atatgcaggt aaaggtcttg caactgtctg taaattagta ggaatcccac cagtactaca
     8341 tatgggttct tgtgttgata taagccgtat actagattta gttggagcag cagctaatta
     8401 cttagatatg gatatgtgtg accttccagt tgtaggtata gctccagagt ggatgtcaga
     8461 aaaagcagtt gctataggat gctatgttgt agcttctggt atagacactt atttaggaat
     8521 aatgcctcca atagctggtt cttcaagagc agtggatata ttaactagtg aattaaaaga
     8581 taaagttgga gctacattta ctgtaaatac aaatccaaaa gagttagcag ctacaataat
     8641 agaagatata gagaaaaaac gtgttcactt tgaagcatta gttgaagaaa aaatggcaga
     8701 aaaggctgaa gcttaaaaaa tagtgaagtt atagaataaa cgtttttata agaagtttct
     8761 ggttgaataa agttgaataa agtcaaatat taaagtaaag aacatcaaaa tataatgaat
     8821 atactgaata aataaaatat aaacaatatt aaagaataat aaaactatac atgctgcttt
     8881 gaaaagctag tagggtaaat ttaattggtt cctgtaagga ctagctgtag tttattttta
     8941 gtgtataaaa atatagagta taaaaaatat ttaactgtaa aattaactac taaaaagcaa
     9001 atagtaataa aaggtggtag ggattatgaa gatagcaata acaggtaaag gtggagtagg
     9061 taaaacgact ttttcttcaa tgctctctag gatgtttgct gaagacggat acagagttgt
     9121 tgctgttgat gccgaccctg atgctaattt agctttagca ctaggttttc caaaagaagt
     9181 atatgaatca atagtaccta tatcagaaat gaaaaaatta gtttcagata gaacagctgc
     9241 ttctgtaggt tcatttggaa aaatgtttaa gatgaatcct aaagtagacg atatccctga
     9301 aaatttctgt aaagaataca atggagtaag attacttaca ttgggaaccg tagattctgg
     9361 tggtactgga tgtgtatgtc cagagcatgt tcttttgaag agactctgct cacatttaat
     9421 actacaaaat aaagatgtag tggttatgga tatggaagcg ggaattgaac acttaggaag
     9481 aggaacagcc caaggtgtag atgcttttat agtagttgta gaaccaggag agagaagtct
     9541 acagacttat agaaaagtta agaaacttgg acatgatata ggtgtaaata aagtatttgt
     9601 agtaggaaat aaaattagaa ataaggaaga tgaagaattt ataatccaaa acttagaaga
     9661 tggagaatct ttaggattta tttattataa ccaagatgtt attgattctg atagagctaa
     9721 tcaatctcca tatgattcta gtgaaacaac gaaagaacag attaaagcaa taaaagataa
     9781 attaatgtca cttaaggata agtaaaatag atagaaattt tgatttggga ttttaagttg
     9841 gttaaaggtt agacataggc aatagatagg taataagtaa gtaattagat aaataactag
     9901 ctattaagtt tgaatatttt aatagcttag tagctagttt ttaagtaatt ttacatgtat
     9961 aattatctta taaaatataa tttttacata ttaatacaat ttttacaaaa attatatttt
    10021 aaatctattt aggttgtggg aggagattca gttatgggat ttaaatctga cattgaaata
    10081 gctcaagaag ctaagcctca agatattaga gaggttgcaa aaaaattagg actaggtgaa
    10141 gatgatgtag aactttatgg taaatacaaa gcaaaagttg actacaatct actaaaaaga
    10201 gagactggta aaaaagctaa gttaatacta actacagcta taaacccaac tccagcaggt
    10261 gaaggaaaaa ctactactac tataggtgtt gctgatgcat ttgctaaact agacaaaaat
    10321 gtattagttg ctttaagaga accatctcta ggaccagtat ttggtgttaa aggtggagca
    10381 gctggtggag gatatgcaca agttgttcct atggaagata taaacttaca cttcacagga
    10441 gattttcacg ctataggagc tgctaacaat cttttagctg ctatgcttga taaccatatt
    10501 caccaaggaa atgcacttag aatagaccct aagaaaataa cttggagaag atgtgtagac
    10561 atgaacgata gacaacttag aaatatcgtt gatggtatgg gtaaaaaagg tgatggagca
    10621 gttagacaag atggattcga tataactgtt gcttctgaaa taatggcagc gttctgtttg
    10681 gcaagtgata tatctgattt aaaagaaaga ttaggaaaca taatagttgg atacagctac
    10741 gaaggtgaac ctgtaacagc tagacaatta aaagctaatg gtgctatggc tgcattatta
    10801 aaagatgctt taaaaccaaa cctagttcaa actcttgaag gaacaccatc atttgtacac
    10861 ggtggaccat tcgcaaatat agctcatgga tgtaactctg ttatagctac aagaatggct
    10921 atgcactttg cagattatgt aataactgag gctggtttcg gtgctgacct tggagcagaa
    10981 aaattcttag atataaaatg tagaatggca aacttaaaac cagatgctgt tataatagtt
    11041 gctactgtaa gagcattaaa atacaatggt ggagttgcta aagcagactt aaataacgaa
    11101 aacctagaag ctcttgaaaa aggacttcca aacttattaa aacatgttga aaacataact
    11161 caagtatatg gattaccagc agttgttgca atcaatagat tcccacttga tactgaagct
    11221 gaacttaaac ttgttgaaga caagtgtaag gaattaggtg ttaatgttgc tctttctgaa
    11281 gtatgggcaa aaggtggaga aggtggaatt gcagtagcta aagaagtttt aagattattg
    11341 gatgaagaag aaaataactt tagattctgc tatgaagatg atttatctat aaaagacaag
    11401 ataaatgcta tagctactaa gatttatgga gcagatggtg tagattatac tcctgaagct
    11461 gataaggaaa tagctaactt agaaaaactt ggatttacta aagttccagt atgtatggct
    11521 aaaactcaat attctttaac tgatgaccaa actaaattag gtagaccaac aggatttaga
    11581 ataactgtaa gacaagcaac aatttcagca ggagctggat ttatagttgc acttactgga
    11641 gaaataatga agatgccagg attaccaaaa gttccagcag cagaaaaaat agatgttgat
    11701 gaaaatggag taatagctgg attattctaa ggaaattaat ttataaataa agagtaggag
    11761 atatcttaaa atagttcgta aaatatctcg aacaatttga gatattttcc tacatccttt
    11821 caaggaggat tatcatgaaa atagcagata aaacttgtgt tgattttgta gaagtactat
    11881 cttccaaaga agcagtacca ggtggtgggg gagctgctgc actagtaggt gcaataggta
    11941 tggctttagg tagtatggta tgtaacctta caatagggaa gaagaagtat gctgagtatg
    12001 aagaaagtgt aaaagagata ttaagtaagg caggaaagtt agaaaaagat ttattaaaaa
    12061 tgatagatga tgatgcagaa tgtttcttac cattatcaaa agcttacggg cttcctaaag
    12121 aaacagaaga agaaaaaaga ataaaagcag aaacaatgga aaaggcatta aaagttgctt
    12181 gtgaagtacc attaaacata gtaagagttt gctatgaagc tattaagtta catgaagatt
    12241 tagttgataa aggttcaaga cttgctataa gtgatgttgg tgtaggagtt cagtgtttaa
    12301 gagctgctat acttggtgga caattaaatg tagttataaa tataaattct atacaagata
    12361 aagaatatgt taataaggta aaaacagaag tagataagtt agttgaagat ggtgtaaaaa
    12421 tttgtgatga agtttatgcc aaagttgaaa aagctcttgg aaaataattt ttaaatgacg
    12481 aaagtagata ctatgttatt aatatgtggt atatagaata aattaattta agtttgcttg
    12541 taggggagtt gtgaatgtat ggaaggaatg tcaactaaag gacaaattat aaaaggaaaa
    12601 cctgtagctg ataagattag tgaagaatta ataaaagaag tcgatttact aattaaagaa
    12661 ggtataaatc ctaaactaac aatagttaga gttggggcaa gaagtgatga tttatcttat
    12721 gaaagaggag cactaaagag atgtcaaaac ataggtataa caacagaagt gttagagtta
    12781 gctgaggata taacacaaga ggaatatatt gatgtattaa agagagttaa tgatgacaaa
    12841 aatgttaatg gtatattatg ttttagacct cttccaaaac acttaaatga agaagttata
    12901 aaatatgtta ttgcaccgga aaaagacgta gattgtttta gtccaataaa ctctgctaag
    12961 gtaatggaag gggataaaag tggatttcca ccatgtacac ctacagcagt agttgagata
    13021 ttaaaacact ataatgttga tttaaaagga agtaaggtaa cagttcttgg aagatcaatg
    13081 gtagttggaa agccagtatc aatgctttta ttaagtgaac atgcaactgt tacaatatgt
    13141 cattcaaaaa ctaaaaatct atcaggtgta gcagcagagg cagacgtgct aatagcagct
    13201 attggtagag ctaagatggt tgatgaaagt tttgttaaag atggagcggt agttatagac
    13261 gttggtataa atgttgatga agaaggaaat ttatgtggag atgttgatac aaatgcagtt
    13321 ttagacaaag tatcaatgat aactccagtt ccagcggggg ttggttcagt tacaacatct
    13381 atacttgcaa aacatgtagt taaggcttgt aaattacaaa acaataaata gatatatata
    13441 tgtggaaggt ttagggggga agtagatatg ataatttctg aaaataaacc attagaagaa
    13501 gtgctagggt acttaaaaga ttttgacaag ctggttttag ttggttgtaa tcaatgtgca
    13561 gcaacttgta aaagtggtgg ggaagaagaa gttttaaaaa tgaaggaaac tcttgaaggt
    13621 gaaggcaaaa aaatcttagg atatgtaatg cttgaccctg cttgtaacct tttaaagtct
    13681 aaaaaggatt taaaggctct aaaggaagag actaaggaag ctgatgcagt tttatcttta
    13741 gcatgtggag atggtacaca gacaatagtt aagaatttaa aagataagcc agtttatcca
    13801 gccaacaata ctttgtttat aggggaagta caaagagttg gagaatatga agaagcctgt
    13861 aaggcttgtg gagactgtga acttggttgg acaggtggga tatgtccagt aactatgtgt
    13921 gctaaaggtc ttatgaatgg ggcttgtggt ggagctaaaa atggtaagtg tgaagtaaac
    13981 tcagagaatg attgtgcttg gataaagata tatgaaagac tagaagctat aggacaatta
    14041 gataacttag cagaaataag accaccaaaa gactattcta agcaaaataa tccaagaagt
    14101 ttaagtgcaa agaaaaagaa agaagcagca gcaaattcat aaaagttttt tttagaaggt
    14161 taaaaggggg attagagatg agcttattaa gagaaacatt agaaagtgga aaatttgcag
    14221 ttactactga aatggctcct ccaaaaggga ctgacctttc acatttaata gaatgtgcaa
    14281 agccgttagt tggaagagtt catgcagcaa atgtaacaga ctttcaatcg gcagttatga
    14341 gagcaacttc tcttgctaca tgtaaattat taaaagatgc tggattagag ccagttattc
    14401 aaataacagg gagagataga aatagaatag ctatacaagg agaaatgttg tcagcaggtg
    14461 tatttgggat aaacaatctg ttggcactaa caggagacca tactagcgta ggagaccatc
    14521 cacaagctaa gggagtgttt gatttagatt ctgtggggat acttcaaact gctgagactt
    14581 taatggcagg tacggacatg gctggtaata agttaaaagg ttctccagat ttttatctgg
    14641 gagcatcagt aactccagaa tattctccaa tagaagttca attattaaaa atgaaaaaga
    14701 aaataaaagc aggggctaaa tttttccaaa cacaagcact ttatgacata aatacaatga
    14761 gaaagtttag agaattaaca agggatatgg attgcaaggt attagcagga atagtacctt
    14821 taaaatcacc aggaatggct aaatttatga ctgcaaatgt accaggaata tttgttccag
    14881 atgaacaaat cgaaagatta agagcagctg gaaaagaaaa ctgggtaagt gaaggaataa
    14941 aaatggcagg ggaacttatc aaacaattaa aagaagaaga tttatgtgat ggtgttcata
    15001 taatggcaat tggggcagaa gagaatatac ctgctatact ggatgccgca ggtttatagt
    15061 attgcaagat tgtagattat attaggtttg ggggaattaa agatgaagat agtagtagta
    15121 ggtggtggac caggaggata tgtagcagct ataaaagctt ctatgcttgg tgcagatgta
    15181 acagttgttg aaaaaagaag agttggagga acttgcttaa atgcaggatg tataccaact
    15241 aaagcacttc ttgcttcttc aggagtattg aatactgtaa aagaagcaaa ggattttggt
    15301 atagaaatag atggtacagt taagccaaac ttcactgcca taatggaaag aaaaaataaa
    15361 gttgtaaacc aattaataag tggtatagag ttcttatttg aaaaaagagg agtaaactta
    15421 gttaatggtt ttggaaaatt aattgataaa aacactatag aagtaactaa agatgatgga
    15481 acagtagaaa ctataaaagc agataagata atactagcaa atggttctgt tccagttgta
    15541 ccaagaatgt tcccatatga tggaaaagtt gtaataacta gtgatgaagt tcttggactt
    15601 gaagagatac ctgagtctat gttaatagta ggtggtggag ttataggatg tgagataggg
    15661 cagttcttta gagccttggg aacagaagta actatagttg agatggttga ccaaatacta
    15721 ttaaatgaag acaaagatgt agctaaacaa ctacttagac aatttaagaa agataaaatt
    15781 aaggttatta caggtattgg agtacaaact tgtgaagtag ttgatggtaa ggctgttgca
    15841 actctttcta atggaaaggt tatagaagca caatatgcat tagtatgtgt tggtagaaga
    15901 cctaaccttg acaattctgg agtagaagat ataggaattg aaatggaaag aggaaaagta
    15961 gtagttaatg aacatcttga aactaatgta gaaggtatat acgcaatagg cgatataata
    16021 gatactcctt tcctagcaca tgttgcttct aaagaaggta tcgtagcagt tgaaaatgct
    16081 ttaggtaaga ctaaggtagt agattataga gcaattccaa gatgtgttta tactgaacct
    16141 gaagttgctg gtgttggtaa gactgaaaag caacttgaag ctgaaggtgt agaatacaat
    16201 gtaggtcaat ttgattttag aggtcttgga aaagcacaag ctataggaca tttccaagga
    16261 tttgtaaaag ttatagctga caaagaaact gataagataa taggtgcagc agtagtagga
    16321 cctcatgcta cagatttatt gacagagttg tcacttgctg ttcatctagg attaacagta
    16381 gaacaagtag gggatgcaat acatccacat ccaagtttat ctgaagggct aatggaagca
    16441 cttcatgatg tacatggaga atgtgttcat tctgtaccta aattataata gacagacaga
    16501 gtacataagg ctctgtggct agatttaagg gggaagtctt aaaatgggat acaatatagc
    16561 tgttgcagga aaaggtggaa ctggtaaaac aagtctcaca gggcttttga ttgattattt
    16621 ggttaaggac aaaaagggac cagtattagt tgtagatgct gatgccaatg ctaacataaa
    16681 tgaagtatta ggtatcgaag ttgaagcaac aataggagaa ataagagaag aagtaaatca
    16741 aagagaaaag ttaggcaatg cattccctgg tggtatgaca aaggcacaat atcttcaatt
    16801 tagattaaat tctataatcg aagaaggcga aggatatgac ttattagtaa tgggtaggtc
    16861 agaaggtgaa ggatgttact gttttgtaaa tgggatactt agagaacaag taaacaaaat
    16921 atctggtcat tacaaatact tagtcatgga caacgaagct ggtatggaac atctaagtag
    16981 aaaagtcact agacatgttg atacactttt attggttagt gattgctcaa gacgtagcat
    17041 acaagctgtt gctagaataa gagatttagc tgaggaacta aagttaagtg ttggaagaat
    17101 acttttgatt gtaaataaag ttccaaatgg ggttatgaat gatggtgtta aagaagaaat
    17161 tgaaaagcat aacctagagc taatcggtgt tgtacctatg gatgaattga tatatgaata
    17221 tgattctaca ggtattcctt tagttaattt acctgaagat tcaaaatcta aagtggctat
    17281 gaaagaaatt tttgctaaat tagaattaaa ataggcttaa gggggaaaaa aatatggcat
    17341 ttaaaatgtc tactcaaaaa tattctggaa aaatatcaga agttgaagta ggaatagggg
    17401 aaaaagcaat taaattaggt ggggaaaatg tattaccttt ttatagtttt gatggagaag
    17461 taggtaattc tccaaaaata gggatacaaa tatcagatgt ttatcctgaa agctggactg
    17521 attcatataa ggaattatac aaagatgtag ctaattgtcc agtagagtgg gctaaatatg
    17581 ttgaagctaa tacacaagca gattttattt gtttaaaatt tgatggttct gacccaaatg
    17641 gattagataa gtcagttgat gaatgtgctg atgtagctaa ggcagtaatt gaagcaataa
    17701 aattaccttt agttgtagct ggttcaggaa atcatgaaaa agatggtaaa ttatttgaaa
    17761 aattagctca aacattggat ggacataact gcttatttat gtcagcagta gaagataact
    17821 acaaaggagt aggagcatca gctggtatgg cttatgcaca taaagtagga gctgaatctt
    17881 ctgttgatat aaacctagct aaacaattaa acgtattgtt aactcaacta ggcgttaaag
    17941 gtgaaaatat agttatgaat gttggatgtt cagcagttgg ttatggatat gagtacgttg
    18001 catctactat ggatagaatt agacttgcag catttggtca aaatgataag acattacaaa
    18061 tgcctatcat aacaccagta gcttttgaag taggtcatgt taaagaagct atagctccaa
    18121 tagaagatga gccagattgg ggttgtccag aagaaagaac tatagcaatg gaagtttcaa
    18181 ctgcagcaag tgttttagta ggtggttcaa acgcagttat acttcgtcat ccaaaatcaa
    18241 tagaaactat aaaagaattg gttaacgcat tagcttaatt aaattgttaa ggttaggggg
    18301 aaaaaataat ggcattaaaa gctttagata tatttaaatt aacaccaaag aaaaactgta
    18361 aggattgtgg attcccaact tgtatggctt tctctatgaa ggttgcttca ggagctgtag
    18421 aagtaggtaa atgcccacat atgtctgatg acgctatagc aaaattatca gaggctactg
    18481 cacctttaat gaaagcttta aaagttggtg ctggtgcatc tgagtatgaa ctaggtggag
    18541 aaacagtatt atttagacat gaaaaaacat tagtaagtag aaatagatat gcagtttcat
    18601 tctgtacttg tatgagtgat gaagcagtag atgctaaaat agctaacatg aaaaaagttg
    18661 actatgtaag aattggtgaa caaatgaaag ttgaaatggc tgttttagag tattgtggag
    18721 ataaagatgc gtatttaaaa ttaatagata aaataaaagg cagtggatta gaagtagctt
    18781 atattttagc ttgtgatgat gcacaagtag ttaaagaagc tgttgaagta ttaaaagatg
    18841 ctagaccaat ggtatatgga gcaactaaag agaactacaa agatatgata gaagtagtta
    18901 aaggagcaag tttaccatta ggtgtaaaag ctggtagctt agaagaatta tatgaaacag
    18961 ttgaattaat tcaagctgct ggatataaag aattagtatt agatgtaaca ggagaaaaca
    19021 taaaagatac ttacactaat gctatacaag ttagaagaac agctttaaaa gagcaagata
    19081 gaacatttgg atatccttca atagtatttg caaatagatt atctaattca aaccctatga
    19141 tggaagttgc tttatcatca atattcacta taaaatacgg ttctataata gtaatagatg
    19201 atattagtta tgctaaggca ttaccattat ttgcactaag acaaaacata tacactgacc
    19261 cacaaagacc aatgagagtt gaaccaaaga tttatccaat caacaaccca gatgaaaatt
    19321 ctccagtatt agttactgtt gactttgcat taacttactt catagttgct ggtgatatag
    19381 aaagatcaaa agttccagta tggttagtaa taccagatgc aggtggatat tcagttctta
    19441 catcttgggc tgctggtaaa tttggtggaa actcaatctc tgctttcatt aaagaaagta
    19501 aggtagaaga agtaactaat tgtaaagacc ttatcattcc aggaaaagtt gcagtactta
    19561 aaggtgatat agaagataac ttaccaggat ggaatgtagt tataggacca gaggaatcta
    19621 tggaattacc taagttctta aaaggatacc aagaaaaagc atgccaaaca aactaataaa
    19681 atattaacta actattaata ttaaatttaa agggggacat tataaatgga aaaatttatg
    19741 attataggtg agagaataca ttgtatatca ccttcaataa gaaaagcatt agcagaaaga
    19801 gacccagctc caatcttaaa aagagcaaaa gagcaattag aagcaggagc acactatata
    19861 gattttaata taggacctgc cgaaagagat ggcgaagaaa taatgacatg gggaatcaaa
    19921 ttacttcaat ctgagttcaa caatgttcct atagcattag atacagctaa taaaaaagct
    19981 atcgaagctg gacttaaagt ttatgataga actaatgcaa aaccaataat aaactctgct
    20041 gatgctggtt caagatttga tttaatagat atagcagctg aatacgaagc aatggttata
    20101 ggtttatgtg caaaagaagg tatcccaaga gataatgatg agcgtatggc ttactgccaa
    20161 gaaatattag aaaaaggctt aatgttagga atggagccaa ctgatatact atttgaccca
    20221 ttatgcttag ttataaaagg tatgcaagaa aaacaagtag aagttttaga agctataaaa
    20281 atgatgactg agatgggact tttaactaca ggaggattat ctaacgtatc taatggatgt
    20341 cctaagcatg ttagacctgt tttagatagt gcattcttag caatggcaat ggctaatgga
    20401 tttagttcag ctataatgaa tccatgtgac ccagaattaa tgaaaactgt aaaatcttgt
    20461 gacataatca acggggcatc tttatatgca gactctttct tagagttaaa tgaaggtgga
    20521 tttgctttct agggttttta gggtatagtt aataaaaaat aggtgtaaat attaaagggg
    20581 gaaatagaat gaatctatat aatataatct ttacagggtc agaacaagct ttaggtgcgg
    20641 ctcaagctat gttagctgaa gctatagaaa agaatggaaa agagcataaa gttgctttcc
    20701 ctgatacagc atattcatta ccttgtatat atgctgcaac aggacaaaag atgaacactt
    20761 taggggactt agaaggtgct ttagaagtag taaaatcttt aataaataga actcatttac
    20821 tagaacatgc ttttaatgct ggtttagcta cagctttagc agcagaagta attgaagcat
    20881 taaaatattc aactatggat gctccatata gtgagccatg tgcaggtcat ataactgacc
    20941 ctataatcag atcacttggt gtaccattag ttacaggaga tatacctggt gttgcagttg
    21001 ttcttggtga gtgtccagat gcagaatcag cagcaaaagt tataaaagat taccaatcta
    21061 aaggtttact aactttctta gttggtaaag ttatagacca agctatagaa gctggagtaa
    21121 aaatgggtct tgagctaaga gttatacctc taggatatga tgtaacttct gttatacacg
    21181 ttgtatctgt tgcagtaaga gcagcattaa tattcggtgg attaactcct ggtgatttaa
    21241 acggattatt agagtacaca gctaatagag ttcctgcatt tgttaatgca tttggaccat
    21301 taagtgaatt agttgtatct gcaggtgctg gagcaatagc tttaggattc ccagttataa
    21361 ctgaccaaac agttcttgaa gttccaatga acctattaac tcaaaaagat tacgataaga
    21421 tagtagctac ttcattagaa gctagaggaa taaaaataaa agttactgaa atacctatcc
    21481 cagtttcatt tgcagcagca tttgaaggtg agagaattag aaagagcgat atgtttgctg
    21541 agtttggtgg aaacagaact gaagcttggg agcttgttgt taagaaagaa gcaacagaag
    21601 ttgaagacca taagatagaa ataataggac caaatataga tgaagttgat gcagatggtg
    21661 tattaagatt accacttgct gtaatagtta aaatagctgg taaaaatatg caagaagatt
    21721 tcgagccagt tcttgaaaga cgtttccact acttcttaaa ctatatagaa ggtgtaatgc
    21781 acgttggaca aagagatatg gcttgggtaa gaatctctaa agatgcattt gataaaggat
    21841 ttagacttga gcatatagga gaagttttat atgctaagat gttagatgaa tttgaatcag
    21901 tagttgataa gtgtgaaata actataatta cagatgctga aaaagtttct gaattaaaag
    21961 gagaagcaat agctaagtac aatgctagag atgaaagatt agcttcatta gttgatgaaa
    22021 gtgttgatac tttctattct tgtaacttat gtcaatcatt tgcaccagct cacgtttgtg
    22081 ttgttactcc tgaaagactt ggtctttgtg gagcagttag ctggttagat gctaaggcta
    22141 ctaaagaatt agacccaaca ggtccttgtc aaccaataga aaaaggcgag tgcttagatg
    22201 atagaacagg tgtatggaat tcagtaaatg aaactgtaaa ccaaatatct caaggtgcag
    22261 ttgaatctgt tacattatac tctatactag aagacccaat gacttcttgt ggatgcttcg
    22321 aatgtatctg tggtataatg ccagaagcaa atggatttgt tgtagttaac agagaatttg
    22381 caagtgttac tccagttggt atgactttcg gagaacttgc ttctatgaca ggtggaggag
    22441 ttcaaactcc aggatttatg ggacacggaa gacatttcat atcttctaaa aaattcgctt
    22501 atgcagaagg tggaccagaa agaatagttt ggatgccaaa agaattaaaa gattatgtag
    22561 ctgataaatt aaatgctaca gttaaagaaa tgactggaat agaaaacttc tgtgatatgg
    22621 tttgtgatga aactatagct gacgattctg aaggagtatt agctttctta gaagaaaaag
    22681 gtcatccagc attagctatg gaaagtgtaa tgtaattgat ggaaaattta aaataatatg
    22741 atataatatt aatatgattt aagtaattta atcaataata taatattggg ggtctatcga
    22801 aatgaaatta ttaccagaat taaaatactc taaagatcat gagtgggtaa aagtaattga
    22861 tggagatgtt gtatatatag gtataacaga ctatgctcaa gatcaattag gagaaatatt
    22921 gtttgttgaa acaccagaag tagaagatac tgtaactaag ggagtagatt ttggagtagt
    22981 tgagtcttct aaagtagctt ctgatttaat atctcctgtt aatggtgagg tattggaagt
    23041 taatgaaaaa ttagaagatg agccagaatg tataaatgaa gacccatatg aaaactggat
    23101 attaaaagta aaactagctg atgtggctga acttgatacg ttattaagtg ataaagaata
    23161 cgaggctgga ttagaataat taaagattaa gatattatta taattgggga aagtatagta
    23221 aaatttgagt gcacgcaaat ttagctatac tttcctttta tcatatatag aacaaattgg
    23281 gagggataac atgataaaag taagttttac accaaacaat aaagaggttt attgtaatga
    23341 gggggatata ttactagagg ttgcaagaaa tgcagatata ttcatagatg ctccttgtaa
    23401 tggaaacgtg tcttgtggga agtgtaaagt aaaattattg aatggaaaag ttgatacaga
    23461 aaaaacaaga catataacag atgatgagtg ggagcaaggt tatatactag cttgttgtac
    23521 aaaggtaata tcagatatag aaattgaagt tccttctaaa gtatcatctt ctatgcatgg
    23581 tatgaagata gaaggtagta ataaaaaaga ggatagggaa atttttgaaa gagctaaaaa
    23641 aataattgaa gaacataacc ttcaatttaa aacaaatata aaaaagaaat acatagaaat
    23701 ggaagaacca aatttagatg ataatataag tgatgtagat agattggaaa gatatgttag
    23761 aaataaccta ggatataatg aaatagattt tagattggat atacttagaa aaatgccaac
    23821 tgtatttaga aagagtgatt ttaaggttac aataacatat gttcaaaaac aaaagaaact
    23881 tacaatcata aatatagaac aaggaaataa agaaaattcc ttatacggag ttgcaataga
    23941 tattggtaca acttcagtag ttgtttgttt agtagaccta tactcaaaag aggtagtaga
    24001 taaagcatct tctggaaatg cacagataaa atatggagca gatgttataa atagaattat
    24061 atattcaact aagaaaaatg gattagagac acttcataaa gctatagttg aggaaactat
    24121 aaatccacta ttaaaaacaa tatatgaaag aaatggaata gataaggaag atgtagttac
    24181 attagtggct gctgggaata caactatgac aagtttattc ttgggtgtgt atacagactt
    24241 cttaagacaa gaaccttaca taccaccatt tttaaaatct ccaaaactta tgggtgaaaa
    24301 tgttggtctt tttgtaaatg acagtgcata tgtgtactta gctccatctg ttgcaagtta
    24361 tgtaggagga gatataacag caggtgtatt atcagcaggg atatggtcaa gtgaagaaaa
    24421 tgttttattt atagatttag gaactaatgg agagattgta tttggaaata aagattatat
    24481 gatgagctgt gcatgttcag caggacctgc ttttgaaggt ggagggataa gctgtggaat
    24541 gagagcatca gcaggagcaa tagagaaagt aattattgat aaagaaactt tagagccaac
    24601 attaaagatt attgatgaat gtgcaccagt tggtatatgt ggttctggta taattgattt
    24661 aatctgtcaa atgattacta aaggggtaat agatagaaga ggtaaaatat acagggattt
    24721 agataataag agagttagat ttaatgagca tgagatagga gagtatgtat tagcatttaa
    24781 agaagaattt gatttagaaa atgatatagt agtcaatgaa gttgatatag ataatttcat
    24841 cagagctaaa ggagctatat attcaggcgc atacactctt gtagatagtt taggtatgga
    24901 ttttagtata ttagataggg tttatatagc aggtggtata ggaaacaacc tagacataga
    24961 aaactcaata attatagggt tacttcctga tattgataga gaaaaattta cttatatagg
    25021 gaatagttca cttgttggtt cttacttagc tcttataagt aaagatgcaa aaaataaact
    25081 tgaagaaata ggtaatcaaa taacttatgt tgaacttagt gtatatccaa gttatatgga
    25141 tgaatttatt tctgcatgtt tcttaccaca tacgaatata gagcagttcc caacagcaaa
    25201 gaaattacta gaagagtaaa atgtacttaa gaaggaataa actatgaata agaatctatc
    25261 tgaaaaaatg gaaatagcat ttgatattaa attaaagaat tttggaaata aaatagagtt
    25321 tgcttatcct aaccagacac tagctcttag tactacaagc aatcaatgta gtttgaaatg
    25381 tgcccattgc aacggtcact acttaaacaa tatggtgcca atagaagaat atgaggaaaa
    25441 agtacaatca aggaatataa ctagctttct attgagtggc ggttgtagtt atgagggaga
    25501 tgtccctata aatactcata ttaataccat taaaaactta aaagagcaag gatatagatt
    25561 gaatgctcat ttaggtctta tggataaaga tagcattgta gagttgtgta agtatttaga
    25621 tatagtttct tttgatttag tgtttgatga tgagacaata agagaagttt ataagatgaa
    25681 aaagagtaaa gaagattata ttgaagtcta taatacaata caagaacata cagaagtagc
    25741 accacatata tgtattggtt taaaaggagg acaaataaag ggtgaatatg aaattataga
    25801 atacctacaa aaaaatcctc ctaataagct tacatttata gtccttattc caacaaaagg
    25861 gactgagtat gaaaatgtag aacctcctga gttagaaggg gtagcagata ttttatgtga
    25921 agctagaatt aatttaccag atactgaaat taatctaggt tgtatgagac caagaggagt
    25981 gtatagaaaa gagctagacc aattatctat aatgtgtgga gtcaatagaa tagtattacc
    26041 atcaaggtct gcaaagaata aggctataga aatgaatatg acaataaatg agtgtaagga
    26101 gtgttgcgtt ttatgattag actatcagtt gggacagcaa tagaacttgg gatattaaac
    26161 aaaaaaagcg atatacctcc taccacagca tatataatga ttggagaaaa atgcataaat
    26221 aaatgcagtt tttgttctca atcaatagaa agtagtacta gaaaagataa attatcaaga
    26281 gtaatttggc cagagttttc aaaagaagaa atattagatg ctttaaaagc ttataaagga
    26341 aaaaatataa agagaatatg tattcaatca atggcaagtg aagaagcaca taactctgtt
    26401 ttagacttta taaactatat aagtggtaag atagacatgc caatttcact atctgcaaaa
    26461 ttagaaaatg atgaacagat aaagaagttt ttttcagtag gagttgataa aataggaata
    26521 gcaatagatg cagcaaataa agaactatat gaaaaaataa aaggaaataa ctatgatgaa
    26581 aagttaaaat ttataactga aatgtcaaaa tcatatccca ataaaataag tacacatata
    26641 atagttggta tgggtgaaag tcatgaagat atatacaatt tatatactta tttaaaggaa
    26701 aatgatgtaa cgattagttt atttgcattt actccagtta gaggaactaa aatggaaaag
    26761 ataaatcaac caagcataga aagttataga agggtacagt taatgtctta tatgataaat
    26821 aaaggttatc caaaagagta ttttaagttt aaaaatggat acttaaatag tataaagctt
    26881 gacaatgata ttttaaaaga cataaacaaa ggctatcctt ttgaaataag aggttgtaag
    26941 gattgtaata gaccatacta taatgaaaga ccaggaagta ctatatacaa ctattcaaga
    27001 cctttaaatc aaagtgagat agatttagca ataagagaaa taaatctata gaggatttct
    27061 aggatatatt tatactaaaa atgggggcgt aataatcatg aatcagtgga gagttataca
    27121 taataaatca tatgaaggag caatgaatat ggcaattgac gaagctatat ttactgcata
    27181 taagaaggga cataataagc caactcttag attttataca tgggaaccag cttgtttgag
    27241 tataggatat tttcagaaat tggaagatga gatagattta gataagtgta gatgtatgaa
    27301 tatagactat actagaagaa ttacaggtgg aagagctgta ttacatgata atgagctaac
    27361 ttatagtata attataggag aagataatcc tttaatcgat aagagcatca acctatctta
    27421 tagatatata agtgaaggtc tagttaaagg gttgaatcta agtggtatag aaacagataa
    27481 tttaaataga ggcgagagaa taagtaggga aaatttatca gctgcatgtt ttaatgcaca
    27541 tgcatcatat gaagttacta ttaataataa gaaggtaatt ggaagtgctc aaagcagaaa
    27601 agatggtgtt ttattacaac atggttcaat aatactggat tttgatgtag aaaaattgtt
    27661 taaattaata aaaactaaga caccagaatt gaaagaaaga gctatgaaat ttacagctaa
    27721 aaaagcaagt ggaatagaaa atgaaattgg cagaaaaata gacatagata ttctacagaa
    27781 aaacatagta aagggattag ctgaacaatt taatgttgag tttgtggagg gcgatttgac
    27841 tgattatgag aagcaattag ttaaagaact atatgaaaaa tacaagaatg aagagtataa
    27901 taaaaagcgt taactttaat tgactttaca taaattaaag tgatatactt aaggtataat
    27961 aatatacaaa aactgaaatc atgaaggttt ctttggtgta ttgaaatacc taagggaatc
    28021 tttttttgat ttttaataaa aatcatatta ggaggagatt tatatgtgtg aatcatcagc
    28081 tttcatttgt aaaagtaata atgagttaga aaaggttatg gaaaatgtgg ttaatataga
    28141 cccttgtgat ggaaagattt atttaacgga tttattagga gagcaaaaga taatagatgg
    28201 gataattaaa gaaataagat tgatggacca taagataata atacaagaaa attaattaat
    28261 aggggaattt catatgataa tagcagtgat agatggaatg ggtggaggta ttggagccca
    28321 gatagtttct tctttgagag aagagttacc tacatatgta gaaatttatg cactagggac
    28381 taattctata gcaactagtt ctatgatgaa agctcatgct aataaaggag ctacagggga
    28441 aaatgctata gtagtatcag ctaaaaaagc aaatataata gttgcaccta tttctgtaat
    28501 aatacctaat tctatgatgg gagaagttac ttgtaatata agtgaagcta ttgcagacag
    28561 tgaggcactt aagatacttt tacctattat gccagaaaat gtggagttag ttggattaga
    28621 aggtaaacca ttagcactac ttgtaaaaga ttctgtaaac cttataaaaa aagagtttaa
    28681 tataaaataa cagggggaat aactaatgat ttttgataaa gagattgtaa gatatcatca
    28741 tggacatcat gaccatgacc actgccacga acatactcat ggagatatat gtcatgaaca
    28801 tccacatgac catgctcacg accacgacca cgaacatagt catgaggaat catcagagtc
    28861 taaggatgaa aaaactttaa aaatactttt agtacattgg ataaatcaca atgaaactca
    28921 tgaagaagga tttagagagt gggttgaaaa ggctagagct attggaaaag aagaaacagc
    28981 aaagagtatt gaaaaggcta tagagtatat ggaagaagct aataaaatgt tgttagaggc
    29041 taaaaaacat atgtaaatat aattaatcgc ttatacgttt gatataagcg atttttatat
    29101 tttgtgatgt ttaattatta aaaattttat ctaaggggtt gtctaaagat ggatataagc
    29161 ctccctttca ttgttgaaat gataaaaata tatctaattt catgcaaaaa agagtatatc
    29221 atgaactaaa aagtttattt tgaggtattt tctgtaatat actttaatga gtaaatttta
    29281 ttacaagatt ggattattta atgttaaata attatatatt ctttaaatac tgcaaaagcc
    29341 tcatcaagca atttgagtta ttttatgtta agtaattata tattgagtat tactatttaa
    29401 agatagtatt aatgttaagt taaaaaataa tattaatgtt aagttgaaaa tagtactaat
    29461 gataagatac ttaaaaggca gtttaaaatt actctaatat tagatataat aaaaattagc
    29521 atgattaaaa atattatttt gaagttaaaa gatttatgca tttatattag attttttctt
    29581 taaaaaagga gtgaattatt tatgaaagat ggtcatatac attcacctta ttgtcctcat
    29641 ggaagtaagg atgattttga aaaatatgta caaagagcaa tggatgttgg aataacagaa
    29701 atgacattta cagaacattt tccgtatgca aatggattta gagaccctgc tcctgaaaat
    29761 gatagttgta tgagtattga agatttacca aagtatttta atgatgtcaa gaagttgaaa
    29821 gaaaagtatg agggtaaaat caagataaat gttgggtcgg aagtggattt tatagaagga
    29881 tacgaagaag ggataaagga gaatttaaat aaatatggaa aggaattaga ggattctctt
    29941 ttatcagtac atataattaa gattgatgat gattattatt gtgtagatta cagtgtagag
    30001 gagtttcaaa atttaatcga caaattagga agtattgaag ctgtttataa taagtattat
    30061 gaaacattga taaaagctgt aaattcagat ttaggtatac ataaaccaaa aagaataggt
    30121 catttaaatt tagttagaaa gtttaatcag gtatttccat acgattataa aggaaataca
    30181 attcttgaag agttaataaa gttaattaaa gaaaaaggtt atgaacttga ttataatgtt
    30241 tctggaaata gaaaagagta ctgcaaagag ccttatatag atggatactt attagagttg
    30301 gtaaaaaaat acgatattcc attaatttta ggttctgatt ctcactgtgc agaggaaata
    30361 aataaatacg atattgttta gatatattca cacaaaagac tgtagataag tttgtataaa
    30421 ataaatttat gtacagtctt ttgtaaattt atatgattga aaatagtaat agaacttaaa
    30481 caaaataata ataagttcta agtaaaataa caatagaatc taaataaaat aataataaaa
    30541 ttcaatagaa taataggact tagaattaat ctatcttatt tatataaata ttcttatata
    30601 agattgtaga taagcttgta tttacacaaa tctatagacc ttctctatta tattgactta
    30661 gtaattataa atattatttt gcgtttaaag ttattatttc ataagtttat cgttttccat
    30721 tattataggg tcaggtaatt caatttcacc ttctgacatc ttcaatctat taaacatata
    30781 attttttaca tcttctttat ttttaaattt agtaacttga taaggtgatg taacaccgta
    30841 tttttctaaa aatagaatgc catttttagt tttaatacat atgcctgtat gacctataaa
    30901 tacgttttta gtttctggat aacgaagaaa tccatttata atagatatat ttttattatt
    30961 tttaaaagat atttttcttt ttttccattc attttttatt tgttctgaaa ttttgattgt
    31021 atcatctgta tctttagctg gtattgcaga aaagaagttg ataaattttt ctacatcttt
    31081 gatgctaaat ttagcatctt tattattcat tatagtgtcc aagtcgacat tcaaatcaat
    31141 agagtctcca gtaaatttct tatttgaatt tataaaatct ttaaaaagtc taaaagcagt
    31201 tattctacaa tttacatcca tataatcagg gaattttttc acccaattat cttgcaattt
    31261 ttctaggtta tatggaactt gttttgcatt gattgaagaa tatccagatt tagatatatt
    31321 tatattattt tgaagtattt taacttgcaa tttattataa ttgttgactg ctttaataaa
    31381 tgtatctatg tctttttcat taattttatt ttcttttagg atttccttta cactattttg
    31441 agttttttta tctatcatat tagaatatat caatttatta ttattatatt taatatttga
    31501 atatgcaaat atcgtaagta tacttgatag tactaagaca acagacaata tcattattat
    31561 taatcttttt ttcatattta attctccttt ttaaatttat tgtgtcgaca tatttatagt
    31621 atatagtacc tttaaattta ccttcaatat gtttttaatt acaaaactgt tgtaaaaaat
    31681 ataactttat ttaaaacttt tttttaatat gtaaatatat atactaatag tgggtactta
    31741 tttttagaat attcagtaaa atgattttga tatactctct atattttgta tgttaaaagt
    31801 aaataaatta ttgttaaaga aggtaaagac ttttaaattt cttatcatag gattttaagt
    31861 aaataagaag gattggagta attaaaaatt tttattttgc aaaaataagt atttatttat
    31921 atggataaat ttgattttaa atctataaat atagataaaa tctaattatt taacttcaac
    31981 tattcctcaa aaaagcaact tttaaggaat agttgaagca aaaagtttgg ttacataata
    32041 gtaattttag ttacaagaat gaaactgtaa aacttaggat tttctttata taatttcatt
    32101 gagaacattt aatttaggag gtaagatatg aaagtaaaaa aatttaaaag tggtatagta
    32161 atggcattaa cagtaatggc aattggagca tcattgacaa atgtatcagc tatggaatta
    32221 aacaagagta aagagtttga aactaaatac aatgaaatga agatggattt tcaagaggaa
    32281 tatggaaaag aagacaaatt taatataaca ttggatgatg aatttacaaa agaagaagta
    32341 aatggagcta aagggattat aaagacaact aataaaatga ataatgaggt tgtagtatta
    32401 aattattttg atgaaatgaa atctggaatt gaatttgagt tgaagccaaa agcagaaaaa
    32461 aatgcaattt tgagagatga atttcaacaa ggttatggaa aggaagataa attcaaagtt
    32521 gtgttagatg atgaatttac aaaagaagag gtaaatggag ctaagggcat tataaagact
    32581 acagataaag agacaggtga ggttatcgta tataattact ttgatgagct tgaaaaataa
    32641 tgcttaaaga ttactttgat tgaatgaaaa ggtaattgtg ttgagaatat aaaatgactg
    32701 aataaaaaca gttgtatcaa aacaaacaat aatagaatgt aagaatgatt gtgttgaaaa
    32761 tattaaatga ctgaataaaa aacgattgtg tcaaaaataa aaaataatta agtgtaagaa
    32821 tgattgtatc aagaatataa aatgactgaa taaaaaacga ttgcgtcaaa aataaaaaac
    32881 aattaaatgt aagaataatt atgtcaaaaa tataaaataa attaaaagaa aaagattata
    32941 ttgaaggaaa aagatttgaa ataggggtga actttacata ttatagttca cccttataca
    33001 aaaatataaa atataattta ttataggaaa atttaaaaaa atcagtctat ttaatttata
    33061 ctgatatatt gtaggatttt acaaacaaac tgttatataa ctttttttat ctccaatttt
    33121 gattttaaga tatctaagaa tttataacaa tcttctggat atatttctcc catatttgct
    33181 acttggaata tattagcatc taggaattta ccttttcctg gatagacaac atagccactt
    33241 tccctttcta gccctaatat aaactcattt acattgattt ctttaggtag aaatacagat
    33301 gttactgtat tagacatata cttatctgga agtaaaaatg ttagacccaa ttcttccata
    33361 ccattcctta aaatagcaga acattctttg tatctttcta ttttactgtc atcttccaaa
    33421 aactcagtta aagcttcatt aagggcaaat attaaagtaa cattaggtgt atttggtgtt
    33481 tgagaagatg atttagcaac tttatagtgt ttataaagat taagatacac attcttacat
    33541 tgattttctt taatattttg taaaatactc tctttagcac aaaggtatgc agaacctgga
    33601 aatgctccaa gagctttgcc accaacacct gttattatat ctatattaaa ctcttccaca
    33661 tcaatatatt cacctgctga ggcacttatt gcatctacaa aaaatatttt gttatacttc
    33721 ttagcaagtt ttcctagttc agggactgga tttatcattc ctgaactagt ttcatggaac
    33781 accatagcaa ttacttttat atcagaattt tcaattatgt atttttctaa aatgtctagg
    33841 tccgggtatt ctgcccattc aaaattaggt ttatatgttt ttacattata agtagataat
    33901 atttcttcta gcctttctcc aaaaacacca ttactaacta gcagaactgc atcttctttg
    33961 tcaaagatag aagatagtac acattcattt acagatgttc cagagccaga tacaattaat
    34021 gagtagtaat tagaagttgc attatatagt ttattgattt tttgttgtgt atctttgaat
    34081 aaatccataa actcatttcc tctatggcat atatcatagt gaagaagtga atttctgact
    34141 ttatctgtaa ccattactgg tccaggactg aatagatatg tttttttcat tgtaaagcct
    34201 ccttaaaaag ttaaatttaa atttttgaat gactgattat gtgtaaatat taaaatatta
    34261 tgtgttattt taactagatt atagaataaa ataataaata ttgcaataat atttcaataa
    34321 aaatactttt ttaatcacta taattaaaaa taacaaaaaa tattgtaatg aatattgcat
    34381 aaatccacat gatgatataa aatataaata acttgaatag attatatatt caaaaaataa
    34441 aaaagaattt attaattctg aaaataaagg gggacattta aagtgaaata tgtattagca
    34501 attgaccaag gaacaacagg aacaagagca gtattgataa acgaaaaatc agagtttgtt
    34561 tttagcgatt atatggaaca tactcaaata tatccacaat ctggatatgt tgaacatgac
    34621 cctatagaaa tttggaataa tacaaaaact gtgagtaata atgtattaca acaggctttt
    34681 gaaaatggaa tcaatgaaag tgatataaaa ggaattggta tagataatca aggtgaaaca
    34741 gtaatgttat gggataaaaa tacaggtaaa ccactttaca atgctattgt gtggcagtgc
    34801 aggagaactt atgattatgt ggaaaattta aagaatatac caggtttaga aaataagata
    34861 atagataaga ctgggttaat tatagaccca tatttctcag gtacaaaaat taaatgggta
    34921 atagataatg taaaaggtgt aaaagaaaaa attaaaaatg gagaagtact tgcaggaaca
    34981 ttagatagtt ggttaatatg gaaaatgaca ggaggaaaat cttttttaac tgatgtatct
    35041 acagcagcaa gaacaatgct ttttaatata agagatatgg attgggatag agagttgctt
    35101 gacattatag gagtacctag aagtatttta gcagatataa tgcctacgag tggtgatttt
    35161 ggaactacag ataaggaggc attttgtggg gtttctataa aaattactgc ttcaatggta
    35221 gaccaaccag cagctttatt tggcaatgga tgttttgaac caggaatggt taaaaatact
    35281 tatggtacag gatgtttcct atatatgaat ataggtgagg aactaaatat agcaggtaat
    35341 ggtattttga caactgttgc ttggaaaata ggagataaag ttacatatgc ttatgatggt
    35401 ggagtgtata tagcaggtgc tgcaatacaa tggcttagag atggcattgg agttataaaa
    35461 aattattcag aaactgatga catggcaaat tccatatcaa gtactggagg tgtatatttt
    35521 gtaccagcat ttgcaggtat agcagcacct tattgggacc aatatgctag aggtacaatg
    35581 gttggaataa caggaagtac taaaaaagag catatagtaa gagcgactct tgagtctgtt
    35641 gcacttcaag tcaaagatgt tgttgattct atgaacttag cctcagggaa aaaaataaaa
    35701 atgttgagag tagatggtgg aattactaaa aataacttta caatgcaatt tcaatccgac
    35761 atattaggta taccaattga aattgcagaa aatcctgaga cgacagtact tggagtagga
    35821 tatatggcag gtctttcttg tggtgtctgg aacagtattg atgaaattag aagtgaattt
    35881 acaataagca aaaaatatat tccaaatatg actactcaaa aaagagaaga aatattactt
    35941 ggatggaacc aagcagtaaa aagagctatg aattgggaaa acgataccaa aaaggtgtgt
    36001 gctaattaag ttacaataga gtattttaca tgtataggag gaattaaaaa tgccctttga
    36061 taaaattata ttatacataa tggctgtggg agtgctttta ggagcgttag atagaataat
    36121 tggaaataaa tttggacttg gcgagcagtt tgaagaaggg tttaattcaa tgggtccatt
    36181 ggctttagga atggttggta tagtttgttt agctccagta atttcagatg tactaggtcc
    36241 tataataata ccaatcttca atctctgtgg agctgaccca tctatgtttg caactatttt
    36301 agcgaatgat atgggagggt atccattggc tatggaactt gcacacaata aagaggcagg
    36361 aatgcttgca ggattggttg tgtcatccat gcttggatgt acattagtat tttcaattcc
    36421 agtaggacta ggtcttatag aatatgaaga cagaccattc tttgcaaaag ggttactgat
    36481 aggcttgata acaatacctt ttggaggtat tataggagga ttaatagcag gatttaactt
    36541 atcaatggta attataaata tgataccagt gataatattg gcagtaatat tggcagtagg
    36601 tttaaaattt aattctacgc ttatgataaa tgggtcatta gtttttggaa aatttatatc
    36661 tatattgata acgataggtt tgggagcagc agcatttcaa gaaataacag gtatagtact
    36721 tataaaaggc atgacaccta taagagaagg tattcaagta attggaagta ttgcaatcgt
    36781 gttattaggt acttttccaa tattacattt attagttaaa gttcttaata aaccacttac
    36841 aatagtgggt ggaaaattag gtatggatgc cactagtgca gcaggattag tttttacttt
    36901 agccaatagt attccagtat ataaaatgat gaaagatatg agtccaaagg ggaaagtagt
    36961 aaatactgct tggctggttt gtgctacagc agctttgggg gaccatttag gatttacagc
    37021 aggagtagtt ccagagatga ttacacctgt tgtaattgga aaattggttg gaggagtatt
    37081 ggcaattgca cttgctatac ttatgacaaa agatttaacc ttggaagaag aacaaagtat
    37141 taaaatgaag aaagatgcag tgtagaataa acaatactta agtcaaattt aaattatttt
    37201 aaagtaggag tttaaataac taaaaataaa ttaaaaagac agacaattaa aaaataatcg
    37261 aataactaaa acttataaat taaataaaat ggcaatcaaa taactaaaaa tgataagcta
    37321 actgaaagta gttacataat taaaatataa agtatagttt agacataaat ggagctaaat
    37381 aaataattat ttggctcctt aattttaatt tattacatca aggaactaaa aatcaatcat
    37441 ataagctaat tatcagctat gactaaaggt attccttttt gagaaaactc tattattata
    37501 ttatggtcta tactagcaga ggtaataata gtatctatat gttctatagg agctactgat
    37561 gcaaatgaat ttataccaaa cttaggaggt tcgacaacta ctatagattt tctagacctt
    37621 tcaatcattt tttttctaac aagtgcttct tctaagtgat agtcagagat tccattttca
    37681 agagtgatac ctccagcgcc taaaatagct ttatcaacat taaattgttc aatagctgac
    37741 agtgtaagcg gaccagaaaa agaaccttca gtttctctaa gatagccccc aagcacatat
    37801 acttttatat tagtattcat aagctcgttt gcaactaaaa ttgagttggt tattacagtt
    37861 agattctttt tatctttcaa attttttgaa acttcaagta cagtagttcc agtatccata
    37921 aaaatagtat catttgtatt tataaactca ctacatttaa tagcaatctg ttttttatcg
    37981 tttgagttta ttttttttct tttaacatag ttaagttcta cgctacttgc tataactggt
    38041 atagcaccac cataaactct ttttaaaaga ccgatactct ccatatgggc taaatctctt
    38101 cgtgcagttt ctattgatat atcaaacatt tgaactatgt catttatttt aattatatta
    38161 tctgatttga gaatacttaa aatcttctca tatctttcct gtggtaacat aaaatatcct
    38221 ccttggacta gtattatttt agttttggca taacttacaa ttaagtcaga gtttaggtat
    38281 atttgttaat gtatgtatat ttatcaaata atattataag tgttatgcaa gataatttta
    38341 ccatatttaa aacttacatt taagcaaaaa agtaaaaata gatgtttaat aattcaaaat
    38401 aactcaaatt ttataaaaca attgactaaa atatgatttt attatataat ttactagtat
    38461 agagatatgg agaagtactc aagtggctca agaggatccc ctgctaaggg attaggctgg
    38521 gtaaccggtg cgagggttcg aatccctcct tctccgccaa ttattaaagg ctatttttat
    38581 taaagatagt ctttttataa tgtcataaat gattagatat gtataactaa agaaaggagg
    38641 gaatagatga caactttaat ttttttcata gtggggatag catctttggt agttgctttt
    38701 gttattgatg ggggacatgt tggagcatta tttgtatttt catcagcact tgttgtgttt
    38761 ggtggaacta taggagctgt tggagcttcg actccattcc ctgtgtttaa aaagtctata
    38821 aagataatgt tattaacttt taaaaataaa aagcctaata acatagaaaa tataatctat
    38881 ttcaaagaaa ttgcaacaaa ggctaggaaa gaggggcttc ttagtattga caaagaaata
    38941 agtgagaatg atgaattaga cccttttatt agaaaagggc ttgaacttat ggtagatgga
    39001 gtggatgcag ttactataag aaatatattg gaatcacaga tacagataac atcaaaacga
    39061 cataaagaag gaatagcaat ttttgattca gcaggtggat atgcacctac actggggata
    39121 ataggtacag ttatgggatt agtacatatt ttaggtaatc tttcaagtaa tccagatgca
    39181 ttaggacctc aaatttctgt tgcatttttg gctactttgt atggtgttgg ttctgccaat
    39241 ttaatatggt taccaatagc atcaaaactt aaggctataa atcaacaaga agtaagtgaa
    39301 agacttttgg tgatagaagc aatattatcc atacttgagg gtgataattc taatttaatg
    39361 acagaaaaat taaaaatatt tttaaattca aaagaactaa ttgaacttga agaaattggt
    39421 ggtagagctg aataatataa aaggatggaa tgtttatgtt gtatgatgaa gatgaaaaag
    39481 aagaaaacaa tgagcgatgg cttttaactt attcagacct cataacatta cttatgattt
    39541 tctttgtaat aatgtattct atgagtaatg ttgatgcgga gaaatataaa caattatctc
    39601 aatctctaaa ttctgctttt ggtggttctt ctggagttat tgaaggtggt aatagcaaga
    39661 tagaaccagt agttgaacca ggaagtaatg atttagatac tttacaaaat gctaagttta
    39721 aaaaaattgg tgaagaaatt cagaagtatt taaacgaaaa tggtatggct aattcagtat
    39781 cattaagagt tcaagataga ggattagtaa taagccttaa agatacaata ctatttgata
    39841 cagggaaagc tattgtgaag gataattcta gagataagat tattcaaata ggtaagatgt
    39901 taaatgaaat gaatagttac atgagagtag aaggccatac agacaatatg tctataaaaa
    39961 attcagagtt taagtcaaat tgggatttat cagttatgag agcaaccaat gttgttcaac
    40021 ttcttattga caatgcaggt atagcaccag ataaattatc tgctgtagga tatggtgagt
    40081 ttagaccaat agcagaaaat tcatctgaag aaggtaggtc aaaaaataga agagttgata
    40141 tagttttagt tgatagtaaa tatgataatg tagaaaatgt ttcaaaggat aaatagttaa
    40201 attagctgtc tcaatacaga attaattcta aaggagatgg cttttttatg cgaaaatcaa
    40261 aaatagatac atagtaagtt acctttttgt aggaatatat ggtatcttaa atatataata
    40321 tttaaataat gtataagctc aattaaaaaa tttatagagg atggaaaata aatgggaaat
    40381 aaaaataaaa atactaaata taaagataaa acaagagaaa ggtataataa aaaagttgag
    40441 aatttaaata aagaaaaatt aaataaaatt aataagcaat ctaagaaagt agataagatt
    40501 gatgatatta ttaataaatc tagtattgta ttatgtgtac ttctagtagt gttttcattg
    40561 ctttacttat ttaaactggt tcaacttaat ataataatga tacttttagt aattacagta
    40621 gctattcaac aaggattggt gtcatttggt gcatttagaa ataaaaatta tgtaaaagct
    40681 ttattttcta tatttgtaat agtatgtttc ttaattgctt ttaaatatgt tgtaaatcct
    40741 gtttaaatat atcaataaac gttctataat taatataaat agtgaagtta cagttttaat
    40801 gctattataa attttataat ttgaaggagt agattttatg aataggtgta agtttgcaaa
    40861 attaattatc acatcaatga ttgttttatt acttacagga tgtagtttaa agaaggaaga
    40921 taattataag ttagacccag aaaatccgac atcaatacaa atttggcatt attataatgg
    40981 acagcagaaa atggcttttg ataagcttgt taaggaattt aatgacactg taggtgctga
    41041 aaaaggaatt attgttgaag cattcagtca aggtaatgta gttgatttat cagagaaagt
    41101 tttagattct gcaaataaaa aagttggttc tgaagatgta ccaaatatat ttgcagcata
    41161 ctcagatact gcatatgaaa tggataaatt agggttacta gtagatatgg ataagtacat
    41221 aagtaaagaa gaattaaatg aatacataga cgaatatgta gaagaaggca gaataaattc
    41281 taaggatgag tttaaacttt tcccaattgc aaaatcaact gaaattttga tgttaaataa
    41341 aactgattgg gataaatttg caaaagctac aaattcaaat ataaatgata taaaaacaat
    41401 tgaaggatta gtagagacat caaaaaaata ttatgaatgg acagatagtt taacgaaaga
    41461 gcctaatgat ggtaaagcat tttttggaag agatgcttta gcaaattata ttattatagg
    41521 aagtaaacaa ctaggagtag aaattttttc tgttaaagat ggtaaagtta cactaaatat
    41581 tgatgaaaaa gttatgaaaa aactatggga taatttctat gtaccatata taaatggata
    41641 ttttgcttct tatggtaagt ttcgttctga tgatgcaaaa acaggtgata tattagctct
    41701 tgtaggttct agttcaggag cagcatactt tccagataga gtaatgctaa atgataatga
    41761 gagttataat attgaaacat ctgtttttca agcaccacgt tttaaaggag aaggaaaata
    41821 tgctgtacaa caaggtgcag gaatggctgt aacaaaatct actaaagaag aagaatatgc
    41881 atcagtagaa tttttaaaat ggtttacaga taaagaacct aatataaaat tctctattgc
    41941 ttctggatac ttaccagtaa agaaaaaaac gaatgatttg gatgttataa aagaagttat
    42001 gaagaaagat aatttgagtt tatctgataa agttgaaggt tcaatagcct catctattga
    42061 acagattaac agttgtaatt tatatactaa taaagcattt gatggtggaa ctaaagctag
    42121 acaaatactt gaatattcaa tgcttgataa agctaaagaa gattcaaata aaataaatga
    42181 attagtaaaa agtggaactt ctaagaagga tgcagtagca caatttaata atgataaaaa
    42241 ttttaaaact tggtttgaaa gttttaaggc tgagcttgaa aatgctataa aaaaatagat
    42301 gaattggcag ttagtgtgtt gacgagcaca aaggtatagg tggaatgggt atggaaaaca
    42361 atctaagaaa aaacaattca ataatgaaaa agatgatatt tcccctaatt attgttatgc
    42421 taattcaaac aagtttattt tgtgccacaa ttttatgggg aggaactatt aagaaattaa
    42481 atgataattc gtttgacatc ttaaatgaaa gagtaattaa tcgtaaaaat tatatacaaa
    42541 atgaaatgtt acaaagatgg tcaaatacaa gtgaaacgga agctacaatt aattcctctg
    42601 taaaaaaagt attaaatgaa aaaaatgcct taattgagga tgtaggcaac aatgaagaaa
    42661 ttaacaaaga tattataaaa agcatatcaa atgatttaat atatcttttg agaaaaaatt
    42721 ctgttacagg agcttttata atcctaagtg acaaaaatat taacgataag tcaatagata
    42781 gtaaaggtga aataagtagg acaggattgt atataaggga tttagaccct aagtttaatc
    42841 caaatgataa ttctgatttg ttaattgaga gaggttcatc agatatagca agaagttttg
    42901 gaatatctat ggacagttat tgggaaccta agttttcatt taaaagtgaa aatgagcaag
    42961 atggagatga ttttttttat aaaccattta gagctgcaat tgataatcca aatgtatcaa
    43021 gttctaattt aggatactgg ggaagtaagt ttttattgac aaatgcaggt gataggctta
    43081 tgacatatac agttcccctt atttatgaag atggaactgt atatggagta cttggaatag
    43141 atttaagtgt aaattattta agagaattac ttccatactc agagataaat ggaaataaaa
    43201 agggtaatta tattttaggt gtagatagaa ataatgatat ggcctttgaa aatgtaattt
    43261 caaatggata ctcattaaaa gaaaaatttt gggatggttt acattctaat ttagagcaga
    43321 aggaatcgta taaaaatata tataagatta aaaataaaga taatgaatgt ggtaatggca
    43381 acatttatgc tagtgtacaa tacttggatt tatataactc caatactcca tttgaaaatg
    43441 aacgttgggc tcttttgggt attattgaag aaaaaagtct tttgtcattt tcaaaaaggg
    43501 tagagaaatt agttataata tctatagcaa tttctttatt aataggtcta ataggtattt
    43561 acattgcatc aagattattt acaaaaccaa ttgttctttt agccaacaaa gttagaaaca
    43621 gtaatccatc aaaaccagta aatcttgata agataaatat atctgaaata gatgaattat
    43681 cttcagctat agagttgctc agtgcaaatg ttgcagattc ttcgtcaaaa ttatcacaga
    43741 ttattgaaat ggtagatatg ccaattgggg cttttgagtt atacaaaaac gcagagaatg
    43801 ttttttgtac aaaagaattt tttagcgtgt ttactactga aatcaaattt gaagaatatg
    43861 gatatatgga aaaaaataag ttcctgaaaa aattaagggg actagataaa tatttgcaag
    43921 aaagttatga aacagaaaat acatatatat ataagtttga atctaaagat aaaaaactta
    43981 agtggataag aatgaatatc tttgaagatg agctaaaggt tcttggtgtt gttgttgatg
    44041 tcacaaaaga atttattgag aaaaggaaga tagaatatga aagagatttt gatattttaa
    44101 cgaatctttt aaataggaga gcttttcatt cacagataca agaaaaattt aagaataaag
    44161 atgaacttaa gatagcagca tttattatgt gggatttaga taatctaaaa tatattaatg
    44221 atacatatgg acatgatgct ggtgatgaat atattaggtg tgctgcagac atacttaaga
    44281 agtttatatc atggaatggt atagtatcta gactttcagg tgatgagttt tatgtattta
    44341 tatatggata tgaaagcaaa gatagtataa gagatattat taattctgtt aagaaacaga
    44401 tgaataatac cttattaaaa cttcatgatg gaacagaatt taaaataagg gcatcagcag
    44461 gaatttcctg gtaccctgat gactcaacca actatgatga attaatgaga tacgcagatt
    44521 ttgctatgta tcaaattaaa aatacaatca agggtgaagt tagtgaattt gatattaata
    44581 tatataataa ggattcattc ttattgagta atagagaaga attaaacaag ttaattgatg
    44641 gtcaattagt tgagtatgca tttcagccta ttgtagattg taaaggtaaa gtatttgcat
    44701 atgaagcact tatgagacct aaaataaaaa cttttagttc accaatagat gtcattagat
    44761 tagctcgttc acagtctaaa ttatatcaaa ttgagcgtat tacatggttt aaagcactta
    44821 aatcatttaa aaaatacaaa gaaaaatttg gcgattgcaa attgttctta aattctattc
    44881 ctaataataa tttgtcaaaa gtagattttg aggaaattga agatttatat ggtgaatatt
    44941 taaatagagt agtactagaa ataacagaga atgataagat taatgaagat tttattgaaa
    45001 aaaagaaagc atatatagac cattggaata tagaaattgc tttagatgat tttgggacag
    45061 gttacaacgg agatgctgta ctattatata taatgccaga ctatgtaaag attgatatgt
    45121 ctattgtaag aggtattgat aaagatgaaa acagacagaa aatattaaaa aatcttatct
    45181 catattctaa cgaaagaaat attaaagtga tagcagaagg tgtagaaaca aaaggtgaaa
    45241 tggaaacact aattaatctt ggagtagatt atatgcaagg ctactatatt agtaaaccat
    45301 catttatacc tgaagatata agcgacaaaa ttaaaaaaga actcaagata tgtcatgaaa
    45361 ataggaatga tgtggtttaa tagaggaaaa attaaattta tttttatgaa cttttagttt
    45421 ataattaaaa agaaaataat ataattataa atcaaggtgg tggagctaat aatcacaaca
    45481 aatatagata taagaaagtt aaatgaaaat caattagaag cagttgagca tatagatggg
    45541 ccttgcatga tacttgcagg gcctggttca ggtaagacca gagtaattac atatagaata
    45601 gccaatatgg ttgtaaacaa aaacatagca ccaacaagaa tcttggcaat aagttttacc
    45661 aaggcatctt caatagaaat gaaaaataga gctttgaatt taagtgatga cataagactt
    45721 aataaggtaa cctatggtac ttttcactct gtatttttta aaattctaag atactttgaa
    45781 agatacaact tagacagtat atttgatgaa aagtcaaagc gaatgacaat caaggcaatc
    45841 ctaaaaagtt taaatataga gaatgctgat gatgatgaga atataggaca agttataaat
    45901 gaaatttcct atgtaaaaaa tgaacttatg gataaaaatg aatttaattc agaagtatta
    45961 acaaaagatg agtttttaaa agtatacaac ttatacgaag aacaaaaatc taaagttaat
    46021 aaaatagatt ttgatgatat gcttataaga acttattatt tacttttgaa caataagtct
    46081 gcactagaaa tggttagaaa tgtgtataaa tatatattga tagatgaatt tcaagatata
    46141 aataaagtac agtttgaagt tttaaagtta atttgtagcc cattaaacaa tatatttgca
    46201 gttggagatg aagaccaaag tatatatgga tttagaggtg ctagaccaga ttttttgctt
    46261 gaatttgaac aatactttaa taatactaaa aaaataatat tagatataaa ttacaggtct
    46321 aaaagtgaga tagtgcatac tgcaaataga cttatcgaca aaaataaaaa tagatatgaa
    46381 aaaatcatta agtgtagtca gggggatgga ggaagtgtta cctatatttc tccacatgat
    46441 tcagaggaag aagccttata tattgccagg gaaataattg acgaaataca aaaagattat
    46501 gtagagtatt cagattttgc agtaatttat agaactaata tacagtcaag agcattagta
    46561 gatgtattta tggatatgag gattccattt gtagtaaaag actcagtaat tacaatatat
    46621 gaccattggg caagtcaaga tatactagcc tatttaagaa taggtattaa tccaaaatca
    46681 aataaagatt ggttgagaat tataaacaag ccttttagat atatatccaa agacagtata
    46741 aatatggtta aggatgaaaa agactttata acagcattaa taaataagtg taatcttcat
    46801 cctaaacaag ttaaaacaat aaatgatttg gaaatagatt taagttattt aaatacttta
    46861 aatcctaaaa atgcaatatc ttatattaga actagtcttg attatgatag atatgtttta
    46921 gattattgta gcaacagaaa gataaagact aatggacttg tagagatatt aaatgaactt
    46981 gagagttcag caactaattt taatactgtt acagagtttt tagagcatat tgatagagta
    47041 aagactgagt taacggaaaa taacaagaat aagcagactg agggtgtcat atttacaact
    47101 atgcatagtg caaaaggatt agaatttaga aatgtttata ttataggtgt aaatgaagga
    47161 acaatacctc atgaaaaatc atatgatatt tgtaaggaag agaaaaaaga agaacaatta
    47221 gaagaagaaa gaagacttat gtatgttgct attacaagag ctgaagaaaa gttatgtata
    47281 agctctactt taaataagta tggtaagaaa gttgataaat cattatttat taatgatata
    47341 aaatctccaa cgaaaaaaga aattgacagt attggtgtag gggataaggt ataccataaa
    47401 aaatttcatg aaggagaaat aattaaaaaa gatggaatta tgtttacaat aaagtttaaa
    47461 gatagagagc gaatcttgga tttgaaaact tgtttattga agaatataat ttatactatt
    47521 taatttagaa tctaaattta gatagaaata taatgtatta tttattatgt gcaagacaaa
    47581 tagatatgtg taaaatgtat tatttaactt attagaaaaa gaaataaggg tttaatatat
    47641 tatttatagt tgtttagaaa aagcaacaat gtggaaaata ttatttagta ttattaaaga
    47701 aaagcaacaa tgtgaaagat attatttagt attatttaag aaatagctaa aaatatgaaa
    47761 tatactattt ataattacac aaataatatc aataaatata aacagtagta tttagataat
    47821 ttacaagtaa aaattattaa aaaaataaaa aacttattga caaaaatatt ataaggaagt
    47881 acaattgtat taacaattta aggaaacaaa tccaatgata gagaaagtag catatatgga
    47941 tttacagcga gttaggggtt ggtgtaagcc taacaatgaa gtctatgtga agagagctct
    48001 ggaggagatt atcagaaact ttagtatgat aattggttta cccgccttaa gggattgaga
    48061 ggataagtac ttacttatca aaaagagtgg taacgcggat ataattcgtc tcttagctgt
    48121 aaagctaagg gacttttttg attttaaaat aaaaatatcc atacagctta aaagctaaca
    48181 gtcacaaatt ttgaaagtaa tataagtatt tatcaataag agtggtaacg cggtaaagtc
    48241 gtctcttagt cattaaagac tgagagactt tttttgtaac tcgaaacatt taagtattaa
    48301 aatctgaggg aaattataat taaaagagag gggtagttaa aatgaaagta tttaaaaaat
    48361 tattaagttt aggattagta ttaggattga cattatcgct agtaggatgt agtggtggag
    48421 gagaaaaaac aaagttagaa caaataaaag acaatggaaa attagttgtt ggtactagtg
    48481 cagaatttcc tccatttgaa tttcataagg tagtagatgg aaaagattca ataaaaggat
    48541 tcgatataat gttggctgaa gaatttgcaa aagagcttgg tgtaaaggtt gaaattaagg
    48601 atatgtcatt tgatggatta ataggtgctt taaatgcaga ccaagttgat attgttcttg
    48661 caggtatgtc tccaacacca gagagagaaa agagtgttga tttttcagaa ttatattatt
    48721 taagtagaaa tgcagttata gttaaagatg cagatataga caaagtaaaa actgaagatg
    48781 atttaaagaa acttagagtt ggagttcaag caggaagtat ccaagaagaa tatgtagtta
    48841 acactttaaa aatgacaact acaaaatctt tgaaagcaat acctgattta attacagaat
    48901 taaaaaatgg aaacatagat gcagttgtta ctaatgaagc agtttcttta ataaatgtta
    48961 aaaaatatga tggaataaaa atggcaaata ctgaagttgg gaaagatgta acagaaggta
    49021 tggcagcagc aatcaaaaaa tctgacaata acaaagattt tatagaatta ttaaacaaaa
    49081 agataaaaga attacaagat ggcaagaaga tagaagaatt tttaaatgaa gcatctactg
    49141 aagctgcttc aaattagtaa gaaagttatg tgggagatga aattatgagt ttagatttta
    49201 gttttttaag tagatttggg acttcttttt tggaaggaac aggtgtgaca gtatcaattt
    49261 ctcttgtggc attatgcttt ggatttataa taggtataat tatctgtatg gcaaaaatat
    49321 caaagagcaa agtattaaga gcaatatcat caatctatat tgaggtttta agagggacac
    49381 cattattagt acagatatat atagtatggt ttggtttacc tcaattagga ataagattcc
    49441 ctatgttgtt tggtatacca agtgaattta tagctagtgc atttgcatta tcagtaaact
    49501 ctggtgcata cgtggcagag atacttagat caggtataca atcagttgat aatggacaaa
    49561 tggaagcaag tagatcatta ggattaaatt attggtcaac tatgagatat ataataatac
    49621 ctcaagctat aaaaaatata ttaccttcat tagcaaatga gtttattaca cttgtaaaag
    49681 aatcttcaat aatatctgta ataggagttg ttgagataat gcgtactgct gatatagtaa
    49741 aaaatgcagc atttagagca ttagagccat taatagtagc agcagcaatt tactttgtta
    49801 taacatttac tctatcaaga ttagttggat tattagaaaa gaaactatct gtgtctaact
    49861 agatgagagc atgtttagca tttagggaaa acatagaata gtaaaaagga aagtgataat
    49921 atgattacaa taaaaaattt aagtaaatct tttggagatt taaatgtatt aaaaaatata
    49981 gacttagaaa tcgctaaagg cgaaataatg gttatagtag gtccaagtgg ttctggtaaa
    50041 agtacacttc ttagatgtat gaatttacta gaaataccta caggtggaga aataatattt
    50101 gaaggaaaaa atttggtcga taaaaaaact aatattgatg aggttagaca aaatataggt
    50161 atggtgtttc agaactttaa tttattccca cataagacta ttttagataa tataacatta
    50221 gcaccaatta aattaaaaaa gatgactaag gaagaagcag aaaaaaaagc agaaattttg
    50281 ttgagtagag ttggtctttt agataaaaaa gattcatatc catcacaatt atctggagga
    50341 caaaagcaaa gaatagcaat agctagagca cttgctatgg aaccagatat gatgttgttt
    50401 gatgagccaa catctgctct tgacccagaa atggtaaacg aagttttgga tgttataaaa
    50461 gaacttgcta aagaaggtat gacaatggca attgtaactc atgagatggg atttgctaag
    50521 gaagttgcag atagagttat ttttatagat ggaggaagta tacttgaaga taatactcca
    50581 gaagaagttt tcggaaatcc aaaacatgaa agaacaaaag cgtttttagc aaaagtgcta
    50641 taatatataa aaaggatgtt attgatatta gaaattcaat acatcctttt ttagtgatgg
    50701 ttaatattaa ttttaaaatt aaagttatat taatattaac tttattttta cgattttttg
    50761 cagaaaaaaa aagagctcgg ggtaagctct ttttttccac cgaattaatt tccgttacta
    50821 atccgatttt taataaatat aaaatatatc cttatgaaag ggtattggga atagatatat
    50881 taactaact
//
LOCUS       DAEPIN010000022        48483 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.022, whole genome shotgun sequence.
ACCESSION   DAEPIN010000022 DAEPIN010000000
VERSION     DAEPIN010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 48483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48483
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.022"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            130..2349
                     /locus_tag="KR250_001491"
     CDS             130..2349
                     /locus_tag="KR250_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HBH0067437.1"
                     /translation="MININYREDQIPIINYDNGTMAVPAVPGAGKTFIITNLVAKLLL
                     EQKHKSGKILILTYMNSAVNNFKGRIRKILEENKIDDTNSYEVMTIHSLAVKIIKEKP
                     EVVMLSEDFNIADDLQKSIILNECISRFRFEGGERAFRWFLKEQKDERWKEITLEAWE
                     RGFFEFAGNAISELKYKGISPDKLEEILSRGHKGMLKIILPIYKLYDRKLKQNGLLDY
                     DDILILAEKTLSLDEGLRKKFQTRYKYIFEDECQDSNEIQGNIIKIISSENKNLVRVG
                     DINQSITGTFSSSDPKFFKEFINSADFCYRMDMSNRSSKDILDLANTLVKYVTKEFRQ
                     EECRHALEDMQIKTVPNGMGYKENPNPEHYNINVKWYESWKIEIEQTARYVKGIKNKY
                     PDKSIGILVPFNEQVTQVAKELRENNLTFEELGPNSLNKRKVINNIAYIIDFMLHCDD
                     VEKLIVVLNKVFIDTDNEEEIKYFLEELKNYSTEEIIYNFEFSKLDNDLIEFKEESDI
                     YLSFKRGIDIIKQILEYPIIRLDLLVLFIGKKLSLEKEDKAVLDYISFYIKYLVSENI
                     NMDLEDVYNLLFDVKNRVFNHIIDVVYEINGYEPEPGSITLCNYHKSKGMEWDCVFLL
                     GLVEYNFPDNINQKFQSDKWYLKEKYKNPMAIIKSEVEAILKGSISTDYAHKTKIDSI
                     NEKIRLLYVGITRAKEMLVLSGSAYRDESDIGNKRKEQKPCIYLSRLNQHIIEKRSN"
     gene            2458..3288
                     /locus_tag="KR250_001492"
     CDS             2458..3288
                     /locus_tag="KR250_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HBH0067438.1"
                     /translation="MNDKLKYFSYSQNSINTYKSCPLKFKYKYIDRINWKNDDVGSRE
                     YYETLKTGRDFHLICERYFSNIPLGIYFNEDDKNSKKFLKWMENIKKVVPIEKGKTYL
                     PEYEVRMTLNGDIIQAKYDLVVIGNNNIEIWDWKTESKKIDYKHVENRIQTIVYMFLA
                     KEVIPKVLKMDINVKDINMRYYQPEFDDLPITILYDEEKHEANRNKIQNYISMIKNTN
                     YEEHTYEDNLYNDIEKVYNKEERMCYRNKKHCKYCEFNKLCNGKEIDYSILEAEIYGT
                     "
     gene            3278..6745
                     /gene="addB"
                     /locus_tag="KR250_001493"
     CDS             3278..6745
                     /gene="addB"
                     /locus_tag="KR250_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddB"
                     /protein_id="HBH0067439.1"
                     /translation="MGLRFVLGRSGSGKSTYILDEIKKEAQKNETTSIILLVPEQYTF
                     EAENRVSKLFLGKEKDKYLRVRVLSFKTLSNIVFSQVGGLTDVNINSSGKAMMVYRAI
                     EDVSEELNVFSKSKSQSGFVSSITDMISEMKQYNISPEMLENISGELDNETLSLKLKD
                     ISKIYNSFEGKLHENYVDAQDMLTSLASKIELSSYLDGACVYIDEFTGFTPNQYNVIK
                     SILNKSKSVNISLTVDDINYIGYSKSDMFSRTKFTYSKLTQLCNEEGIKILPQVNLNT
                     GVIKRFEKVKELQHLERFYNAYPYKIYSNQTENIKIKEFNNLYSEVEEIAREIVHLVR
                     DKNVRYRDITIATRDLNRYDFLVHSIFNEYNIPNFIDKKREAKSNPIVILIISALEMK
                     NRRYGYETMFRYLKSGLIGIDNDDINLLENYVLANGIKGKKWFDEKWDYRITQSLSGQ
                     ESEFELELKEKINEIKNRVLEPIVILQEKLRGKNRVKEICRYIYEFLLDINMPETIES
                     LIINFKDKGELDVANQYSQVWDIVVDILDQMVELMGDEIISLEKFIKLITLGFDEYEL
                     GLVPPSIDQVLVSSVDRMKNPDTKYLYLVGTTDGVFPLITKDSGILSDNDRESLGNKG
                     IEVDIDSKTRSFEEQFLVYKALTSTSKNLTITYPISDHEGKTLRPSIIISRLKKIFPN
                     IENKSYLVEENKSTDKDILKKITVKSPTFNELINVIKNYDSDGYNTEEINSIWLDIYR
                     YYLKDEIYSSITKKVIKGLSYTNQVHKIEEKKIRSLYKSNSLSISRLEKYAECPFAYF
                     IQYGLKAKKRKEYSFTPPDLGTFIHNILDRFSKELLQDNLTWRDIDEKYIELKIGIIV
                     DEIILKIPGYILNSSERYKYLAYRLKNMLTTAITIISQQIKQGSFEPIDYEVKFGNNG
                     KYPPIKMVLENGQEVSLIGQIDRVDEFEEGENKYIRIIDYKSGNKSISLTEIYYGLQL
                     QLLVYLDAILESAKDEDININPAAILYCRINNPIAKFNEDKDDEEIQEAILKELRMKG
                     LVVKDSHIVKEMDKSLIDGERKNSLVIPVGLTKDGNVGKSTSAISYEDFKLLRKYVRH
                     AIKDLCEEMLSGEIRIAPYKHKDGTSCDFCDYSAICQFDSTIKDNKYKNLNNKSNEEI
                     IKMMKGDVN"
     gene            6745..10572
                     /gene="addA"
                     /locus_tag="KR250_001494"
     CDS             6745..10572
                     /gene="addA"
                     /locus_tag="KR250_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HBH0067440.1"
                     /translation="MSSPKWTKEQLEVIESRECNLLVAAAAGSGKTAVLVERIIQMIT
                     SRENPIDIDKLLVVTFTNAAASEMRERIGDAIGKALDENPENKHLQNQLVLLNKSSIT
                     TIHSFCLDVIKSNFHRINLDPNFRIGDQTECAILKQEAIEEVFEDLYEERDEGFLNLV
                     ESYAERGGDKEVQDIILGIYSFAMASPEPKKWLIDSAERFNIDENFDFSQSIWARAIL
                     DTVKIEINGLCLNMERALKEVESIEELETFAEKLSVEYKKIADISQACNKSWDEAYKK
                     MASMSFENYVKGVKRISKDAPSYIKESKEKAKTIRDKTKKSLESIVSATFNKDNDSIR
                     EEIKYLYNIVKPISSVVLRFEEEYSNKKREKGIIDFNDIEHFALNILTDVDEKGNIVP
                     SDIAVGYRNKFYEIFIDEYQDSNLVQEVLLKAVANTETPNRFMVGDVKQSIYRFRQAK
                     PELFLQKYNNYNDKKGSSHRKIMLYKNFRSREEVVDAVNYIFENIMNENIGEIEYTEK
                     ERLNLGANFNVDTDEKSIIGGATEIHLIQKDNKLDDDIINDKDDRINNKENEIEEEEK
                     LDNIQLEARMVGNIIKDLMKVNEDGKIQKVYDKGIDGYRPVEFRDIVILLRATSAWAP
                     VFADELMNMDIPTYADVGVGYFDTIEIKTILSLLQIIDNPMQDIPLISVLKSPIFGFT
                     PEDLIDIRVQSKDKIFYEVLKSTAEYDGFTDSQNENESEFIPSEECINKSKDFLIKLK
                     EFKEKSMYMSTDEFIWYLYTRTGYYAYVGALPGGSQRQANLKVLFERAKQFEETSLKG
                     IFNFVNFIEKLKKSSSDMGSAKTLGENANVVRIMSIHKSKGLEFPVVICSAMGKNFNT
                     QDFKKSILYHHNLGYGPQFVDYERRISFPSIAKEALKSKINIENLSEEMRVLYVAFTR
                     AKEKLIITGSTRNIQDSIKRWSNGIESLDTISQYEILKGKNFLDWIMPCVLRHRDLSN
                     LLEEVGLDAVFNVEHNSKWYGKLWNKSDILVEKKSDEEKESIEEILEKIDVDNPDSDY
                     YSEIEEKLNYIYPYEFSTRKPATISVTEIKKIQNNYEEELINTIFEQKVILKKPLFIQ
                     NEEEREKISGTERGTIVHLVMEVLDLKNVSSVNDIKSQIRGFVSKGIITEKQASIVNP
                     YKIYKFFASNIGKRMLNAEIINREKSIYAQVNMKDIYIYEKLINNDDKKLYDNESVML
                     RGIVDAYFEEDNQIVLVDYKTDFVNEENINQIIEKYKKQLDLYADIIETLTGKSVKEK
                     CIYLFGVDEAVCY"
     gene            10618..11829
                     /locus_tag="KR250_001495"
     CDS             10618..11829
                     /locus_tag="KR250_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HBH0067441.1"
                     /translation="MRFIHTSDWHLGKSLEGHSRIEEQAKFCEEFIKIVESNEIDMVI
                     IAGDVYDTSNPPAQAEKLFYQTVSRLANNGQRCVLIISGNHDNPERLSAITPLAHEQG
                     ILIYGYPLSATIEAKYKGFEITYATQGCTKLNINGENIVVATLPYPSEKRLNEVFSSE
                     DEFEKQKNYSEKVGDIFRSLEENFRSDTINIAVSHIFVIGGESTESERPIQLGGSFLV
                     ERKDLPEKAQYTALGHLHKQQKASERLNAYYSGSPLQYSKDERAYTKGAYIVDIKAGE
                     KPIIEDVYFNNYKPIEVFKCNGIEEALDICEQNQDREIWSYFEINTDEIISQNEIKKM
                     KELLKDIIEIKPIITSCYEQESVDIKEKSMAELFREFYSFSKGVEPKGELMDLFLDII
                     SEEGESADETN"
     gene            11816..15343
                     /locus_tag="KR250_001496"
     CDS             11816..15343
                     /locus_tag="KR250_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBH0067442.1"
                     /translation="MRPIRLELTGLNSYIDKQVIDFEKLIERGLFGIFGTTGSGKSTI
                     LDAITIAMYGNISRNTKEYINSVCDKAIISYEFEIGSKNTRRRYIVDRTIARSKTGTK
                     TSNARLIEVLNDNTQNVLADKVVEVNEKVAQVVGLTANDFTRSVVLPQDKFNEFLRLS
                     RADRRDMLERIFNLEKYGRSLGEKVKKRKNIQLQNLKDLKSKLSQYDGITEEVYNNIN
                     QELIELKNLEKDKNNALDLAQKSYEESKTVYEEQLKLEKNELRKNELDLKNSEIKEKI
                     ALVENDSNAKKVDPYISSVQNLEKKIDEDSFAVSNLEKKLAILNQELEVTKNRYEKIN
                     KIKNEEIPKLSEEKIRLQQAIKLEEELVLLDRELKELKESGINLNKTKVELEKVKQVS
                     ESRKDAVTKSIKEVEGKIDKVNISAELKQKIFLAYEYEKDYNNVLEEKNQKLNKLEEI
                     LKETENINLKVRYIDKDKNDVNRNLENLSLHLDVLLKKCPGKSADLLLKSEYVTELRN
                     KANNTKENEIKKSSIQDELKIILESKFNTEREVNLLNEKLENNRKNRDDLEKELEELK
                     YLNLASELRRELKENMPCPVCGSKHHENHDITKYDENISFVKEKLEKLEKEKISIRNN
                     IEELNAKVSGYLSIEKMKTKELEDVKGKLGGIPSSQLLKKLDEEQRKLALLKSNIQEW
                     EKEKESTENKVTLAKEEKNKIEKEELKIRESLNNYKKLTRDLNIEIEGLENKCKKLKQ
                     EYLGLKTITKVSNLLSKVKEIRENEKELELLSTGHSNLLKNRDSLDIKIRENESKLHE
                     IEIELIKARELYVEKKLSRDNKYKEVISITKGDLAKNLLHNVEENICKILEQEESSKK
                     KLEEQRLEYEKNIAEKHNIDGRLKTAKEQYKDQKSTLNKLLAENKFESIYAVKRALLD
                     TDAVKKLIEEILEHEEEQKLLSFKIKSSKEKLNGRSIKKEYFEQLKDEIYNLKVEIGN
                     ISKDIGANQNQLITLKDSLDKINDFNKQLKVVEHNVDLLEELDKCVQGNKFVEYVSTN
                     QLKYIALEASKRLDGITKGRYALEIDSTLNFVMRDNFNGGERRSIDTLSGGETFLTSL
                     SLALALSSQIQLKGSAPLEFFFLDEGFGSLDSELLEIVIESLERLHSNNLSVGIISHV
                     EELKNRVPVKLLVSSSEAGIGSKVKIEYS"
     gene            15474..16727
                     /locus_tag="KR250_001497"
     CDS             15474..16727
                     /locus_tag="KR250_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBH0067443.1"
                     /translation="MLESPNNLIQIIFLIVLLIGSAFFSASETALMSLSKIRIRYMQD
                     EGVKGAKLVSSLIENPNKLLSSILVGNNVVNIAATSISTSLFIGLMGEKGVALATAVM
                     TVLVLIFGEITPKTIAANNSEKVSLLVSKPIKAIIFILRPIVWIFNIITNIIFKLFGI
                     TNKGAKSFITEEELKTMVNVSHEEGVLEMEEREIINNVFEFGDMQAKNAMVQRIDMVA
                     IDMEDSYDEIIQVFKTEKLSRMPVYEETIDDIVGILNIKDIIFLSDEEIESFDIKNYM
                     REPFFTYEFKKITQLLEEMKLEKSQMAIVVDEYGGTSGLLTIEDLVEVIVGDIEDEYD
                     EEEDEIQVIKEDEYIVDGSTKIGDVNELIGVNLESEEFDSIGGFIIGHLSRLPEENEV
                     IEVDNIRFCIESIEKNRIKKIRIYT"
     gene            16876..17928
                     /gene="ytvI"
                     /locus_tag="KR250_001498"
     CDS             16876..17928
                     /gene="ytvI"
                     /locus_tag="KR250_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YtvI"
                     /protein_id="HBH0067444.1"
                     /translation="MSILENNFLYKLKNNTIFFCIYTLLFIFIYKAFPYIAPFFLGAI
                     IALMINPISQKLENKFHINKGISTLVLSFLAVAIVSTVTTIIVINSMKELMGFLNNIS
                     ANPEDISNTIMYFLNKINDFMKSFQEIANFDVEQLVNKFSGEVMQITKNLLTSILGLA
                     TSIPYIIIFIITLFIATYFIAKDLDKIENSFYNMFTVDVRKKVKNVKKEAGLSLVGYI
                     RAYTILMAITFFAIWGSFALFGLKYGLIVGFVGALMDLIPFLGIITIYLPVIVYYFLI
                     KNYFIAISMTVIFFVLSLIREILEPKLVSVNVGLNPLATLAAIFIGIQVKGIIGVIFC
                     LGLVCMHDILKKVDIF"
     gene            18018..19244
                     /gene="pepT"
                     /locus_tag="KR250_001499"
     CDS             18018..19244
                     /gene="pepT"
                     /locus_tag="KR250_001499"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HBH0067445.1"
                     /translation="MKQKVVERFLKYVSFDTTSNSQCENCPSSEGQRVLAKYIVEELK
                     TMGVDDVSLDENSYIMATLKGNTDGVDTIGFISHLDTIEDVSGKDIKPKIIENYDGKD
                     IVLNEALNVITYVKDSPELEEFKGDDLIVTDGTTLLGSDDKAGIAEIVTAIEYLINHP
                     EIKHGDIKIGFTPDEEIGRGADLFDVEKFGAKYAYTLDGGIVGELECENFNAANATIT
                     IHGRNVHPGSAKNKMVNAIHIAAEISEMFPADERPETTEGYEGFWHLNSIGGNVENVS
                     MAYIIRDHCKEKFENRKSIMIENIEKINKKYDNRVELDLKDSYYNMKEKIEPVMFIVD
                     IAKEAMEELGIKPRLVPVRGGTDGARLSFNGLPCPNIFTGGLNFHGKNECIPVSSMEK
                     ATKLIVRIAEKYAERV"
     gene            19451..20503
                     /locus_tag="KR250_001500"
     CDS             19451..20503
                     /locus_tag="KR250_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp18"
                     /protein_id="HBH0067446.1"
                     /translation="MKIKSKKIIAFALSLGIMLPSCVYADNISKNQNAQVDMNNYLQG
                     RKSPKEEKLIGEDRFDTAIKISQSGWNNGSERVFLVNSNSLPDALASTPLASKLDAPI
                     LLTNKNSIPYNVTEEIKRLNPAEVILIGSEGAISSSVKESLEDMEVLVSRIGGADREE
                     TSLLLTRQLDDTGDLGVSKVAVVNGYNGLADATSISSPAASDNTAIIYTGKDSIRSEA
                     KNFITQNSTKTYIVGGEYSISKKLEGQLVNSERLAGTDRKDTNAKVLEKFYGKSSKVN
                     NMYFAKDGSGREADLVDGLAAGVLASKTKSPVVLASGSLSSAQKSFIKKVKADKFVQV
                     GGGKNSKPYAEALALQ"
     gene            complement(20921..21490)
                     /gene="yfcE"
                     /locus_tag="KR250_001501"
     CDS             complement(20921..21490)
                     /gene="yfcE"
                     /locus_tag="KR250_001501"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HBH0067447.1"
                     /translation="MKIGIMSDTHGSLLYFEKALNVLSDCDVLLHGGDVLYHGPRNDI
                     PEGYNPKKFIETLNKLENIVIVKGNCDADVDQMVIEHPIQSPYVMSQFGEIRIILNHG
                     YIESEEEIIDKAKKMGGDILVLGHTHVKKLYMDDNLIVINPGSTSIPKDGSHSVAIID
                     IIKTDDEDELELDINLIDINTGNIININD"
     gene            complement(21515..22315)
                     /locus_tag="KR250_001502"
     CDS             complement(21515..22315)
                     /locus_tag="KR250_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HBH0067448.1"
                     /translation="MKRIITINTNQLNKSLETKELLTRKLINAGFEVYSDIYPDTELI
                     ISIGGDGSFLRTVRDFDFPEIPIMGINTGHLGFFPDILPDKIDSFIEAYTKKDYIIQE
                     MSLLNAEVYTTTSGSNMLAVNEVVIRGDKSRTIHLNLSLDNKHIQNFSGDGMIISTST
                     GSTAYNYSAGGSIVDINLELMQITPLHPINTNAYRCFTSSIICSNESVIKIAPEYRFE
                     DSVLIVVDGVEHRFRQIENIKVSISDAKIKLLRMSNYEFWHRVSEKFL"
     gene            22450..23310
                     /locus_tag="KR250_001503"
     CDS             22450..23310
                     /locus_tag="KR250_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0067449.1"
                     /translation="MFSIRNITKKLGKFKLNNINLELKEGDIVGIIGPNGSGKTTLIK
                     IIMGIIDADEGEIELCNETIENSPISFKNNIGFVYDSLQFYPHLKVKEFRKIVSLFYK
                     NFDRERFDEYLNKFDIEENMHIENLSKGQSEKLMLSSALSHNAKLLILDEPTAGIDPI
                     VRTEIMQYLQDFVKNGSSSVIISTHNTDNLIKIADYLVFINRGNQIFTVKKELIEQEY
                     KIIRANKAELEAIKESIVGVKEYKYYNEALVKVGDSLKVKSLLIEIDKHKVKNPTIEE
                     LMYYYVNEVR"
     gene            23310..23996
                     /locus_tag="KR250_001504"
     CDS             23310..23996
                     /locus_tag="KR250_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HBH0067450.1"
                     /translation="MFNLIRKDLIIGISSDGIRNLKYILLFFVFYFFLNSISYYTVSI
                     VVSYLIFINTFECDYENDSRIFIRSMPVSIEDIVYSKYLLGVGLIISVTIIVSLLSKL
                     TSLVFFRNMVLNDVFFSVNIFLAILTILLPLIFKFGYGKMKVCGFIVSILIYFVYGSL
                     LKMISMIVYQVKHFNYSKVGGVYLSNYVTDIANTKYINLYSMTFLTIIIFIISMYFSI
                     KISKKNKFNY"
     gene            24048..24590
                     /locus_tag="KR250_001505"
     CDS             24048..24590
                     /locus_tag="KR250_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0067451.1"
                     /translation="MKKFIKVSFFILGIFTLFIAINKLVYRDMVSVKDYEINHLEEIV
                     FDFDSRRSRDVGTIVRFKISNNSKYSYKLNSAKMKFENCVENKKGMNKCSAYVNVDLF
                     NEKNSITERTLKYGIKPNNQGYVDFVIPKGLNFDYRYFNESGMSVEYKGEYIVNIPMA
                     KGLYLVVGKNNDTWNANIME"
     gene            24669..25136
                     /locus_tag="KR250_001506"
     CDS             24669..25136
                     /locus_tag="KR250_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HBH0067452.1"
                     /translation="MSISNVREYFKQFGKEDSILEFEQSSATVELAAEAAGVIPARIA
                     KTLSFKIGDDAILIVTAGDAKIDNKKYKAEFNCKAKMLTPEEVLEFTGHAIGGVCPFG
                     LKNSIKVYLDDSMKRFDTVFPACGSSNSAIELTCEEMEKFSKCEKWVDVCKNW"
     gene            25845..26981
                     /locus_tag="KR250_001507"
     CDS             25845..26981
                     /locus_tag="KR250_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HBH0067453.1"
                     /translation="MDLNSKKYQMLKELYVSFAENEVKPLATELDEEERFPYETVEKM
                     AKAGMMGIPYPKEYGGEGGDTVGYIMAVEELSRVCGTTGVILSAHTSLGSWPIYQYGN
                     EEQKQKFLRPLASGEKLGAFGLTEPNAGTDASGQQTTAVLDGDEYILNGSKIFITNAI
                     AGDIYVVMAMTDKSKGNKGISAFIVEKGTPGFSFGVKEKKMGIRGSATSELIFEDCRI
                     PKENLLGKEGQGFKIAMSTLDGGRIGIAAQALGLAQGALDETVKYVKERVQFGRPLSK
                     FQNTQFQLADMEVKVQAARHLVYQAAINKDLGKPYGVEAAMAKLFAAETAMEVTTKAV
                     QLHGGYGYTRDYPVERMMRDAKITEIYEGTSEVQRMVISGKLLK"
     gene            27040..27822
                     /locus_tag="KR250_001508"
     CDS             27040..27822
                     /locus_tag="KR250_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009524639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HBH0067454.1"
                     /translation="MNIVVCIKQVPDTTEVKLDPNTGTLIRDGVPSIINPDDKAGLEE
                     AIKLKEEMGAHVTVITMGPPQADMALKEALAMGADRGILLTDRAFAGADTWATSSALA
                     GALKNIDFDIIIAGRQAIDGDTAQVGPQIAEHLNLPSITYAEEIKTEGEYVLVKRQFE
                     DCCHDLKVKMPCLITTLKDMNTPRYMKVGRIYDAFENDVVETWTVKDIEVDPSNLGLK
                     GSPTSVFKSFTKSVKPAGTIYNEDAKTSAGIIIDKLKEKYII"
     gene            27841..28851
                     /locus_tag="KR250_001509"
     CDS             27841..28851
                     /locus_tag="KR250_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HBH0067455.1"
                     /translation="MGNVLVVIEQRENVIQTVSLELLGKATEIAKDYDTKVSALLLGS
                     KVEGLIDTLAHYGADEVIVVDDEALAVYTTEPYTKAAYEAIKAADPIVVLFGATSIGR
                     DLAPRVSARIHTGLTADCTGLAVAEDTKLLLMTRPAFGGNIMATIVCKDFRPQMSTVR
                     PGVMKKNEPDETKEAVINRFKVEFNDADKLVQVVQVIKEAKKQVKIEDAKILVSAGRG
                     MGGKENLDILYELAEIIGGEVSGSRATIDAGWLDKARQVGQTGKTVRPDLYIACGISG
                     AIQHIAGMEDAEFIVAINKNPEAPIFKYADVGIVGDVHKVLPELISQLSVAKEKGEVL
                     AN"
     gene            28870..29667
                     /locus_tag="KR250_001510"
     CDS             28870..29667
                     /locus_tag="KR250_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HBH0067456.1"
                     /translation="MSTSDVKVYENVAVEVDGNICTVKMNRPKALNAINSKTLEELYE
                     VFVDINNDETIDVVILTGEGKAFVAGADIAYMKDLDAVAAKDFSILGAKAFGEIENSK
                     KVVIAAVNGFALGGGCELAMACDIRIASAKAKFGQPEVTLGITPGYGGTQRLTRLVGM
                     AKAKELIFTGQVIKADEAEKIGLVNRVVEPDILIEEVEKLAKIIAKNAQLAVRYSKEA
                     IQLGAQTDINTGIDIESNLFGLCFSTKDQKEGMSAFVEKREANFIKG"
     gene            29765..30610
                     /locus_tag="KR250_001511"
     CDS             29765..30610
                     /locus_tag="KR250_001511"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HBH0067457.1"
                     /translation="MKLAVIGSGTMGSGIVQTFASCGHDVCLKSRTQGAIDKCLALLD
                     KNLTKLVTKGKMDEATKAEILSHVSSTTNYEDLKDMDLIIEASVEDMNIKKDVFKLLD
                     ELCKEDTILATNTSSLSITEIASSTKRPDKVIGMHFFNPVPMMKLVEVISGQLTSKVT
                     FDTVFELSKSINKVPVDVSESPGFVVNRILIPMINEAVGIYADGVASKEEIDEAMKLG
                     ANHPMGPLALGDLIGLDVVLAIMNVLYTEFGDTKYRPHPLLAKMVRANQLGRKTKIGF
                     YDYNK"
     gene            30697..31872
                     /locus_tag="KR250_001512"
     CDS             30697..31872
                     /locus_tag="KR250_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000786547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HBH0067458.1"
                     /translation="MREVVIASAARTAVGSFGGAFKSVSAVELGVTAAKEAIKRANIT
                     PDMIDESLLGGVLTAGLGQNIARQIALGAGIPVEKPAMTINIVCGSGLRSVSMASQLI
                     ALGDADIMLVGGAENMSMSPYLVPSARYGARMGDAAFVDSMIKDGLSDIFNNYHMGIT
                     AENIAEQWNITREEQDELALASQNKAEKAQAEGKFDEEIVPVVIKGRKGDTVVDKDEY
                     IKPGTTMEKLAKLRPAFKKDGTVTAGNASGINDGAAMLVVMAKEKAEELGIEPLATIV
                     SYGTAGVDPKIMGYGPVPATKKALEAANMTIEDIDLVEANEAFAAQSVAVIRDLNIDM
                     NKVNVNGGAIAIGHPIGCSGARILTTLLYEMKRRDAKTGLATLCIGGGMGTTLIVKR"
     gene            32068..32970
                     /locus_tag="KR250_001513"
     CDS             32068..32970
                     /locus_tag="KR250_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBH0067459.1"
                     /translation="MFKKENQRYNLISYTNEEEMTLKEVLLDKLNFSVRSLSKMKREK
                     SVLVNGVYKKPSLKVYSGDLIEVKIDEEKANFEPQDLNLQIIYDDFDIIMVNKPPFMV
                     VHPTKSHYDKTIANGISYYIDNQKENVKIRFVNRLDMNTSGLVIVAKNAYAHHTLSTA
                     MSENKVEKKYITVVDGIIKENEGTIDEPIYRPTEDSIKRIIDERGQSSVTHYKVIERL
                     ENATVLEVSLETGRTHQIRVHMAHIGHGIIGDELYGYVDEDLINRQALHAYKLEFEQP
                     RTKEKLKFKADIPEDMKELISKLR"
     gene            32984..33988
                     /locus_tag="KR250_001514"
     CDS             32984..33988
                     /locus_tag="KR250_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HBH0067460.1"
                     /translation="MIIHKFIIHVLDKNSDVPILNDFEGKVNQEVDGFFQKAIKRIAK
                     DEDLRKGVFKDYNDNLIKNCCEQIIYDESTFLKNSKEIASYLFDVMKINAALESCDLA
                     ICLYTIKDEKSVAILKLDYKKLYTHSIEFVDDKFNIQMVSNEIGIPETLRQKQGALIS
                     LSGINDEFHLRLLDKDAEKEGSESKFVTEFLNAKKIDDDKYKTKVFKNTAENWITNAL
                     SNDIKQAEDVRSILNYTLKEKHEVDINDFVDNSIKDDELKDSFKEHMEEKGLDESFSI
                     DKKWVEKKLKKRSIKTDNGFDIKGNLTDFEDPMKYTVKQNQDGTIDIIIKNVTFYEEK
                     "
     gene            complement(34132..34359)
                     /gene="acpP"
                     /locus_tag="KR250_001515"
     CDS             complement(34132..34359)
                     /gene="acpP"
                     /locus_tag="KR250_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBH0067461.1"
                     /translation="MFEKIKEIIAEQLGVDNLDEITMEASLMDDLEADSLDAVEIIMA
                     LEDEFGIEIPDEEAENFKCIGDICKYIEENK"
     gene            34531..34956
                     /locus_tag="KR250_001516"
     CDS             34531..34956
                     /locus_tag="KR250_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0067462.1"
                     /translation="MKYVGLLLTSVGMFLLIAVNFYYNSITLDMQRIEDYVMETNLIL
                     EDVAEKESYVSNEKEDYISRLMHVKKGIENSKTSFLIERYKEYKIKSIESLIYTISEE
                     KKDYLDEVDRYNKLGEKEINKLINKNFLEVTYLSITTYI"
     gene            35004..35210
                     /locus_tag="KR250_001517"
     CDS             35004..35210
                     /locus_tag="KR250_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0067463.1"
                     /translation="MTKLEDNMERFFGGCEGFFGNKMLIIIVIVFLLLCTDILEDLLC
                     DDNIWIWVILIVLLLFNFDDGCCC"
     gene            35392..35571
                     /locus_tag="KR250_001518"
     CDS             35392..35571
                     /locus_tag="KR250_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0067464.1"
                     /translation="MLDRFFGEEGCFGGGGWWIIVLFFLFLAFGDSWAEFDIMAWIPF
                     LIVLLILCCCGGCLE"
     gene            35605..35805
                     /locus_tag="KR250_001519"
     CDS             35605..35805
                     /locus_tag="KR250_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0067465.1"
                     /translation="MLDRFFGEEGCFGGGGWWIIVLFFLFLAFEDCWRDIDIMSWIPF
                     LILLLITCSCGGFFDGEVGCGC"
     gene            36058..37074
                     /gene="ccpA"
                     /locus_tag="KR250_001520"
     CDS             36058..37074
                     /gene="ccpA"
                     /locus_tag="KR250_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator CcpA"
                     /protein_id="HBH0067466.1"
                     /translation="MKGNITIKDVAKQAGVSISTVSRVINDSKPVTDEVKQKVLEVIK
                     ETGYIPNPLARSLVTKKSQLIGVIVPEVSDSFVNEVLNGIEEVAKMYDYDILLANTYS
                     DKEQELKSINLLRAKQVEGIVMISWIVEQEHINYIQNCGIPATYISKTARNYDIYTVS
                     TSNEEATFDMTEHLIKKGHEKIAFIMTSKDDTVLEMERLAGYEKALSNNNIELDKSLI
                     KYGGTDYESGYNSMKELLDDGIIPHAAFVTGDEAAIGAINAICDAGYKVPEDISVAGF
                     NDVKIARMYRPKLTTVYQPLYDMGAVAIRMVIKLINKELIENKKIELPYRIVDRESVT
                     ERKK"
     gene            complement(37197..37595)
                     /locus_tag="KR250_001521"
     CDS             complement(37197..37595)
                     /locus_tag="KR250_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0067467.1"
                     /translation="MKKLDTESLEKMAKQKNIDKDKIEKMADSYKGKSENELMEELIK
                     IGKNLDGRDEVVSKFKAFLDENQRKKLDNIMEKISDAENQRDTKPAKTKKAKPTKGKS
                     NSPAPKNTPGSQQKKSKSLFKKTKKSNPHE"
     gene            37807..38712
                     /locus_tag="KR250_001522"
     CDS             37807..38712
                     /locus_tag="KR250_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0067468.1"
                     /translation="MNNLLFLIGVIALSNGNISIFDDKNKSKGNNRVRKNKKNANDKL
                     SQSTHGNLRFDANDIKRGLRLMDLSEEDLEMGMEIITRTKKYMSRDERKILIKIESVL
                     DLVRGIKKLNNIDMVDTEDETDFFRKMDSDDKKNMMIKEIIDVFPEKRKNSVEKAIGM
                     KKKIDLFAELFLPDDFGEGGFSLSSLANINNLGSMNNLKLLGSLLRGDDSNNDDEDNN
                     DELNYEDEELDCEDKELDDDELTDEGEMEDNEDELVEDKNTGYENIENDEDIVYDEEL
                     YNINEQESEYNYNEKNTKTRAKKKR"
     gene            38827..39007
                     /gene="ssrS"
                     /locus_tag="KR250_001523"
     ncRNA           38827..39007
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="KR250_001523"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            39190..40407
                     /gene="cdeC"
                     /locus_tag="KR250_001524"
     CDS             39190..40407
                     /gene="cdeC"
                     /locus_tag="KR250_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosporium morphogenetic protein CdeC"
                     /protein_id="HBH0067469.1"
                     /translation="MQDYKKNKRRMMNQPMSTMNEEEVYTDEINSEDMRGFKKSHHHN
                     GCNTDNKCECHDDCNPCNPCNPCKPNPCNPCKPNPCDDNCGCHDNCKCDCEPCEMDSD
                     ECFENKCGPECCNPISPRNFSVSNAVPFAIEANRIFDTMQFQTFTDATGPNGEPLTFE
                     TEVVEVFGSVPSAGQASVTIEKICLSNDGIVIDTGMTTLEDFDLDPLGDIVGRNCETT
                     FEFAVCGERNSECCRQGKGKSVAYKQRGLTVAVRNLVLELRGRCGCTEFVALAFPAVR
                     AGGGCKRRVDYVEFTFNTLSAPICLPADGRAVTLRQEYQTNLTVDCIGKSILKLECNE
                     CCEPFYELIIPNDIDLVLCLQETVSTLISEQIVVLASPNPIQPRLVDTFSKVCDFSQC
                     GPNHGSGKPSCHR"
     gene            complement(40507..41829)
                     /locus_tag="KR250_001525"
     CDS             complement(40507..41829)
                     /locus_tag="KR250_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBH0067470.1"
                     /translation="MKVPYLVLSTVILFISNFIVRIFGFLYKIFLSRALGETGLGIYH
                     MIFNFLMICLAVTTTGIPTALSCLVAKRKALNDRHNTNALFISTLYISFFVALIISIV
                     ASFNSSFLSLKFLKDAKLNLFILAVCPAIVIITLSNVLRGYYYGIKNVKIPAIGQIIE
                     QIGKILFVFLLVMYINNKSMNCYIALLGISIGELSNIIFMLICLWRDSSFDNRYIISI
                     KDFYNSSMETLKMSIPITCNRMSNILLQSISSMMIPSRLALSGMTYQQSLSMYGIVSG
                     MVMPFIFLPFTVGSALIVNLIPTISQEMALRKRKSVIKKIKYSILLTLFVGILSSIFF
                     YFFGKDLCILVFKNTLAGEYLKAMFLVPLFMSLNQTLSGILHSIRKELASSINTITGM
                     LIQLIALYVFLPIPGLNIYAYIYTMTIVSIFTCLLHTIVLFKSLKSIR"
     gene            complement(41880..42674)
                     /locus_tag="KR250_001526"
     CDS             complement(41880..42674)
                     /locus_tag="KR250_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HBH0067471.1"
                     /translation="MFYDYHMHSSFSTDGKSTMEEMVKKSIELGLEEICFTDHVDYDV
                     YADDSFSIVYEDYFKSLETLQSKYKDKISIKKGIEFGVQTQLMDTYKKEAHQYPLDFI
                     ICSIHAIDTMDLYLGNYFKDKTQHEVYENYYLYLYNIVKNYKDYSVLGHLDLIKRYAP
                     YDTILDDRLFSDIIEETLKQAIYDGKGIEINTSCYRYNLPDLTPSKYILQMYKDLGGE
                     IITTGSDSHHISQVACEFDYIYSLLKNMGFKYVSKFNKLKPEFIKL"
     gene            42784..44229
                     /locus_tag="KR250_001527"
     CDS             42784..44229
                     /locus_tag="KR250_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBH0067472.1"
                     /translation="MEKYRIDLNEKKSTKYIQIFNHIKELIINKELVEHEKLPPIRKL
                     SNFLQVNNTTIVKVYELLENEGYVYKIIGSGTFVSSLNSYKSGIDNRKNKIIVQREDL
                     IHFDNGNPSNDMFPIDSFKNAVNMALSKDGSSIFEYDEGLGSEELREKMVEYLSDGNI
                     KTTKENIQIISGAQQGIDIVCKGLISYSDVVFMEEPSYNGAIEVFKSRGAKIISIPML
                     DDGIDIGILKLKLEKIKPRLIYIMPNFQNPTGISYSTYKKKKLIELAEEHDFYIIEDD
                     FISDFVFESKDNRTIRSYDDKNRVVYIKSFSKILMPGLRIGIVEMPNELLKKVLWAKY
                     SSDISTPGLIQKSMYYYMNNFDWKNYLSHIEKIYTDKYKLAKNLINDKLSGKLKVRKS
                     CGGINFFLELPRGYTSQAFTNFMLNKGVSMLPGTYFFDNLVDDRFFRINIARPSMEEL
                     EKGISIISDNIDEFFCEYKNKLDIKSNKLFY"
     gene            44258..44467
                     /locus_tag="KR250_001528"
     CDS             44258..44467
                     /locus_tag="KR250_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 domain-containing protein"
                     /protein_id="HBH0067473.1"
                     /translation="MFDKKKLDRINELAKKNKEGILSADEIKEREILRKEYLENFRAH
                     FRSRLDSVKVVSPEEYEQYMKNNKN"
     gene            44487..45881
                     /locus_tag="KR250_001529"
     CDS             44487..45881
                     /locus_tag="KR250_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBH0067474.1"
                     /translation="MNLKYEFKNAWDFERKENTIEQIMQYSSNYMEFLSKSKTERLSV
                     KEIIKLAKENNYISIDEAMEKGSISCGDKIYVINKEKAVALFVIGKNYIEKGMKIIGS
                     HIDSPRLDLKPNPLYQESNLGFFKTHYYGGIKKYQWTAIPLALHGIVILNDGTKVDIS
                     IGEEDSDPVFCVTDLLIHLAGDQMQKKLSEGISGEALNILIGNMPLEDEEKEPITANI
                     LKILNEKYNIVEEDLLSAEIEVVPAGKARDLGLDRSMVLGYGHDDRVCSYAAVKAILE
                     TEQPEFTSVALCVDKEEIGSKGNTGMHSKFFENTVAELIALEGDYCDIKVRRALANSK
                     VLSADVSAGYDPNFGEAYEKRNSAYMGNGVVLTKYTGSRGKSGCNDANAEFMSEVKRI
                     FNKGNVVWQTAELGKVDQGGGGTIAHILANQGAEVIDCGVGVLNMHAPHEIVSKVDIY
                     EMYKGYKAFFNINL"
     gene            46121..46306
                     /locus_tag="KR250_001530"
                     /pseudo
     CDS             46121..46306
                     /locus_tag="KR250_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896033.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
     gene            complement(46415..47581)
                     /locus_tag="KR250_001531"
     CDS             complement(46415..47581)
                     /locus_tag="KR250_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017752472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBH0067475.1"
                     /translation="MKGGVRKRGKKWYYYFDAGIVDGKRKKVERVGGNTKKEAEKSLR
                     DAINEYENAGIVFDETNMSLSDYLNFWYKEYVLLNCKYNTQESYRNLIENHIEPRLGK
                     CKLKSINPAIIQEFLNNKSKETYTQNGEEKHYTKGVLKAIYVVLNAALKSAVYPYKLI
                     KENPVQYASIPKNVLKVKNESDNKTITLDEFNKILEIYPKNTNIYIPLLIGFHTGMRK
                     GEILGLCWDNVDLDNNIIKVRKNLIKRKVSEFELASPKTKTSIRDIKIGDTLSRILKE
                     EKLNQKKQKIKIGKWYKETEYDWVCRKKDGSFVNHNNIDAAIRTINKKLNINFNFHCL
                     RHTHATLLLENGANVKYIQQRLGHSQLSTTMDTYSHVTSKMESETIDILEGILQ"
     gene            complement(47719..47925)
                     /locus_tag="KR250_001532"
     CDS             complement(47719..47925)
                     /locus_tag="KR250_001532"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0067476.1"
                     /translation="MNLSFNKKKKFELSKEELELIENWLGNKYAGDMTIPAIVDFSLE
                     TDIEYEKIVVYLAEKVLESRDKKH"
     gene            complement(47984..48208)
                     /locus_tag="KR250_001533"
                     /pseudo
     CDS             complement(47984..48208)
                     /locus_tag="KR250_001533"
                     /inference="COORDINATES: protein motif:HMM:NF024835.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
BASE COUNT        19572 a         5086 c         8523 g        15302 t
ORIGIN      
        1 tatataattt gttattaaat gcaaagaaaa tctatgcatt taaaagtgaa tatgcgatta
       61 atggttatat ccaagagagt atattatata gtatactttt aaaaatctca cataaaggag
      121 ataaaaatta tgattaatat aaattataga gaagaccaga taccaataat aaattacgat
      181 aatggtacta tggcagtacc agcagtacca ggtgctggaa aaacatttat aataacaaat
      241 ttagtagcta aactattact tgaacagaaa cataagagtg gtaaaatatt aattcttaca
      301 tatatgaata gtgctgtaaa taattttaaa ggtagaataa gaaagattct tgaagagaat
      361 aaaatagatg atactaattc gtatgaagta atgactatac acagtttggc tgtaaaaata
      421 ataaaggaaa agccagaagt tgtaatgtta agtgaggatt ttaacatagc tgacgatttg
      481 caaaaaagta ttatattaaa tgaatgtata agtagattta gatttgaagg tggagaaaga
      541 gcttttagat ggtttttaaa ggagcaaaaa gatgaacgct ggaaagaaat aactcttgaa
      601 gcctgggaaa gaggtttttt tgaatttgca ggaaatgcaa taagtgaact taagtataag
      661 ggaatttctc cagataaact tgaagaaata ttgtcaagag gacacaaagg aatgcttaaa
      721 attattttac caatatataa attgtatgat agaaaattaa agcaaaatgg attgctagat
      781 tatgatgata tattaatttt agcagaaaaa actttaagct tagacgaagg gcttagaaag
      841 aagtttcaaa ctagatacaa atatatattt gaagatgagt gtcaggactc aaatgaaatt
      901 caaggtaata taataaaaat tatatctagc gaaaataaaa acctagttag agttggagat
      961 ataaatcaga gtataacagg aacattttca tcatcagacc ctaaattttt taaagaattt
     1021 ataaatagtg cagatttctg ttataggatg gatatgtcaa atcgaagttc aaaagatata
     1081 cttgatttag caaacactct tgtcaagtat gttacaaagg aatttagaca agaagaatgt
     1141 agacatgctc ttgaagatat gcaaataaaa actgtgccaa atggaatggg atataaagaa
     1201 aaccctaatc cagaacatta taatataaat gtaaagtggt atgagagttg gaaaattgaa
     1261 atagagcaaa ctgctagata cgttaagggt ataaaaaata aatatccaga taaaagtata
     1321 ggtatattgg ttccatttaa tgaacaagtg acacaagttg ctaaagaact tagagagaat
     1381 aatctaacct ttgaagaatt aggaccaaat tctttgaata aaaggaaggt tataaacaac
     1441 atagcatata ttattgattt tatgttacat tgtgatgatg tagaaaagtt aatagttgta
     1501 cttaataaag tttttataga tactgataat gaagaagaaa taaagtattt tttagaagaa
     1561 ttaaaaaact attctaccga agaaattatt tataattttg aatttagtaa gttagataat
     1621 gatttaatag aatttaaaga agaaagtgat atatacttaa gttttaaaag aggaatagat
     1681 attataaaac agatattaga atatccaata ataaggttag acctcttagt tttgtttatt
     1741 gggaaaaaac ttagtttaga aaaagaagat aaagctgtac tggattatat atctttttat
     1801 ataaagtatc tagtatcaga aaatataaat atggatttag aagatgtata taatttattg
     1861 tttgatgtaa agaacagggt gtttaatcat ataatagatg tggtatatga gataaatgga
     1921 tatgaaccag agcctggtag tataacacta tgtaactatc ataaatcaaa ggggatggaa
     1981 tgggattgtg tgtttttact aggtcttgtt gaatataatt tccctgacaa tattaatcaa
     2041 aagtttcaga gtgataaatg gtatctaaaa gaaaaatata aaaaccctat ggcaattatt
     2101 aaatcagaag ttgaagctat attaaaagga agtatttcaa cagattatgc tcataaaacg
     2161 aagattgatt ctataaatga aaagattaga cttttatatg tagggattac tagagcaaag
     2221 gaaatgttag tactttcagg aagtgcttat agagatgaaa gtgatatagg gaataagaga
     2281 aaagagcaaa aaccatgtat atatttaagc agacttaatc aacatattat tgaaaaaagg
     2341 tcaaattaat atataataag ttattaaaag attgtttgaa atatatctaa aagaggataa
     2401 aacaatatgt acagtaagta actactaaaa aataaagtta agagagaagt gatatgagtg
     2461 aacgataaat taaaatattt ttcttatagt caaaattcaa ttaacacata taaatcatgt
     2521 ccattgaaat ttaaatataa atatattgat agaataaatt ggaaaaatga tgatgttggt
     2581 agtagagagt attatgaaac tttaaaaaca ggaagagatt ttcatttgat ttgtgaaaga
     2641 tattttagca atatacctct tggaatctat tttaatgagg atgataaaaa tagtaaaaag
     2701 tttttaaagt ggatggaaaa tataaaaaaa gttgttccta ttgaaaaagg aaaaacgtat
     2761 ttgccagaat atgaagtaag gatgacatta aatggggata taatacaggc aaagtatgat
     2821 ttagttgtaa ttggaaataa caatatagaa atatgggatt ggaaaaccga gtctaaaaag
     2881 attgactata aacacgtaga aaatagaata caaactattg tttatatgtt tttagcaaaa
     2941 gaagtaatac ctaaagtttt aaaaatggac ataaatgtta aagatataaa tatgagatat
     3001 tatcaacctg aatttgatga cttaccaatt accattttat atgatgaaga aaaacatgaa
     3061 gcaaatagaa ataaaattca aaattatata agtatgataa aaaacactaa ttatgaagaa
     3121 catacatacg aagacaatct atacaatgat atagaaaagg tatataataa agaagaacgt
     3181 atgtgctata gaaataaaaa gcattgcaag tattgtgaat ttaataagtt atgtaatggc
     3241 aaagaaatag attatagtat tttggaggcg gaaatttatg ggacttaggt ttgtattagg
     3301 tagaagtgga agtggaaaat ccacctatat actagatgag attaaaaaag aagctcaaaa
     3361 aaatgaaacg acatcaatta tacttttagt tccagaacag tacacatttg aggctgaaaa
     3421 tagagtaagt aaactatttt taggaaaaga aaaagataag tatctaagag ttagggtact
     3481 tagctttaag acactaagta atatagtttt ttcacaagta ggaggattga cagatgtaaa
     3541 tataaattct agtggaaaag caatgatggt atatagagct attgaagatg taagtgaaga
     3601 attaaatgta ttttcaaaat caaaatcgca gtctggtttt gtaagctcaa ttacagatat
     3661 gatatcagaa atgaaacaat acaatataag ccctgaaatg ttagaaaata tatctggaga
     3721 attggataat gaaactttaa gtttaaaatt aaaagatata agtaaaatat ataattcttt
     3781 tgaagggaaa ttgcatgaaa attatgtaga tgctcaagat atgttaactt cattggcaag
     3841 taaaattgaa ttaagcagtt atttagatgg agcttgtgtt tatatagatg aatttacagg
     3901 ttttacacca aatcagtaca atgtcataaa gagtatatta aataagtcta aaagtgttaa
     3961 tatctctttg actgtagatg atatcaacta tattgggtac agtaaatctg atatgttttc
     4021 cagaacgaaa tttacttatt caaaattgac tcaattgtgc aacgaagaag gcataaaaat
     4081 attgccacaa gtaaatttaa acacaggtgt tataaaaaga tttgaaaaag ttaaggaatt
     4141 acagcactta gaaagatttt ataatgctta tccatataaa atatattcaa atcaaactga
     4201 aaatataaag ataaaagagt ttaacaattt atattcagag gtggaagaga tagccagaga
     4261 aattgtacat ttagttagag ataagaatgt cagatataga gatataacca ttgctacaag
     4321 agatttaaat agatatgatt ttttagtaca ctctatattt aatgaatata atataccaaa
     4381 ctttatagat aagaagagag aggcaaaaag taatccaata gttattttaa ttatttcagc
     4441 tttagaaatg aagaatagac gatatgggta tgaaactatg tttaggtatt taaaaagtgg
     4501 acttatagga attgataatg atgatataaa tttactagaa aattatgtgt tggctaatgg
     4561 gattaaaggt aaaaaatggt ttgatgaaaa atgggattat agaataaccc aaagtctttc
     4621 aggtcaagaa agtgaatttg aactggaatt aaaagagaaa ataaatgaaa taaaaaatag
     4681 agtattagag cctatagtaa ttttacaaga aaaattaaga ggaaaaaata gagtaaaaga
     4741 aatctgtaga tatatatatg aatttttatt agatataaat atgccagaaa ctatagaaag
     4801 tttaataata aattttaagg ataaagggga acttgatgtt gcaaatcaat actcacaagt
     4861 ttgggacata gtagtggaca tattagacca aatggtggaa cttatgggtg atgaaataat
     4921 ttctttagaa aaatttataa aattaataac cttaggtttt gatgaatatg aattaggact
     4981 ggttcctcct agtatagacc aggtacttgt aagtagtgtg gatagaatga aaaatccaga
     5041 cacgaaatat ttatacctag ttggaactac tgatggtgtg tttccgctga ttacaaagga
     5101 tagtggaata ttgagtgata atgataggga aagtcttgga aacaaaggaa tcgaagttga
     5161 tattgatagt aagactagga gttttgaaga acagtttctt gtatataaag ctcttacttc
     5221 gacatctaag aatttaacca taacatatcc aatatcagac catgaaggta aaacgcttag
     5281 accatcaata attatatcaa gacttaagaa gatatttcca aatatagaaa ataaaagtta
     5341 tttagtggaa gaaaataaaa gcactgataa agatatactt aaaaaaataa cggttaaatc
     5401 ccctacattt aatgaactta taaacgtaat aaaaaattat gacagtgatg gttacaatac
     5461 agaagagatt aatagtattt ggttagatat atataggtac tatttaaaag atgaaatcta
     5521 cagttctata acaaaaaaag ttataaaagg tcttagttat actaatcaag ttcataagat
     5581 tgaagagaaa aaaataaggt cattgtataa aagtaattct cttagcatat ctaggcttga
     5641 aaaatatgct gaatgtccat ttgcttattt tatacaatat ggtctaaaag caaaaaagcg
     5701 taaagagtat tcatttacac caccagattt aggaactttt attcataata ttttagatag
     5761 gttttcaaaa gaacttttac aagataattt aacttggaga gatattgatg aaaaatatat
     5821 agaattaaag ataggtataa tagttgatga aataatttta aagataccag gatatatatt
     5881 aaatagttct gagaggtata aataccttgc atatagatta aagaatatgt tgacaactgc
     5941 tataactata attagtcagc aaataaaaca aggttcattt gaaccaatag attatgaagt
     6001 taaatttggc aacaatggaa aatatccacc tataaaaatg gtgttagaaa atggacaaga
     6061 agtaagttta attggacaaa tagatagagt tgatgaattt gaagaaggag aaaataaata
     6121 tataagaata atagattata agtcaggcaa taagtccata agtctaacag aaatatatta
     6181 tggtctacaa ttacaattac ttgtgtattt agatgcaatc cttgaaagtg caaaggatga
     6241 agatataaat ataaatccag ctgcaatact atattgtaga ataaataatc caatagcaaa
     6301 atttaatgaa gataaagatg atgaagaaat acaagaagca atattaaaag aacttagaat
     6361 gaaggggctt gtagtaaaag actctcacat agttaaagag atggataaat cattaataga
     6421 tggagaaagg aaaaactctt tagtaatacc agtaggtctt actaaggatg gaaatgtagg
     6481 taaaagtact tcagcaataa gctatgagga tttcaaactg cttagaaaat atgtaaggca
     6541 tgctataaag gatttatgtg aagaaatgtt aagtggagaa atacgtatag ctccatataa
     6601 acataaagat ggaacatcat gtgacttttg tgattactct gctatatgtc agtttgattc
     6661 aactataaaa gataacaagt ataaaaattt aaataataag agtaatgaag agataataaa
     6721 aatgatgaaa ggagatgtta attagtgagt tctcctaaat ggacaaaaga gcaattagaa
     6781 gttatagaaa gtagagaatg caatttattg gttgctgctg ctgctggttc aggaaaaaca
     6841 gccgttctag ttgagcgtat aattcaaatg ataacaagta gagaaaatcc aattgatata
     6901 gataagttat tagttgttac atttactaat gctgctgcgt cagaaatgag agagaggata
     6961 ggggatgcta taggaaaagc cttagatgaa aacccagaaa ataaacattt acaaaaccaa
     7021 cttgttttat taaataaatc tagtattaca acaatacact cattctgttt ggatgttata
     7081 aaatcaaact ttcatagaat aaacttagac cctaatttca gaataggaga ccaaactgaa
     7141 tgtgctattt tgaaacaaga agcaatagag gaagtatttg aagatttata tgaagaaaga
     7201 gatgaggggt ttttaaattt agttgaaagt tatgcagaaa gaggaggaga taaagaagta
     7261 caagatatta tcttgggaat atattcattt gcaatggctt ctcctgaacc taaaaaatgg
     7321 ttgattgact cagcagaacg atttaatatt gatgaaaatt ttgatttttc acagtctatt
     7381 tgggctagag ctatattaga tactgtaaaa atagagatta atggtctttg tctaaatatg
     7441 gaaagggcat taaaagaagt ggaatcaata gaagaattag aaacttttgc agaaaagtta
     7501 tccgttgaat ataaaaaaat tgctgacatt tcacaagcat gtaataaatc ttgggatgag
     7561 gcatataaaa agatggcaag tatgtccttt gaaaattatg taaaaggagt aaaaagaatt
     7621 tcaaaagatg ctccttcgta tataaaagaa tcaaaagaaa aggcaaaaac tataagagat
     7681 aagactaaga agtctttaga aagtatagta agtgctacat tcaataaaga taatgattct
     7741 attagagaag aaataaaata tctttataac atagttaaac caatatctag tgttgtactg
     7801 agatttgaag aggaatatag caataagaaa agagaaaaag gaataataga ttttaacgat
     7861 atagagcatt ttgcattaaa tatacttaca gatgtagatg aaaaaggtaa tattgtacct
     7921 tctgatatag cagttggata tagaaacaag ttttatgaaa tatttataga tgaatatcag
     7981 gatagtaatc tagtgcagga agtactgctt aaagctgttg caaatacaga aactccaaat
     8041 agatttatgg ttggtgatgt aaaacagagt atatatagat ttagacaagc aaagccagaa
     8101 ttatttttgc aaaaatataa taactataat gataaaaagg gaagctccca tagaaaaata
     8161 atgctttaca aaaactttag aagtagagaa gaagttgtag atgctgtaaa ttatatattt
     8221 gaaaatataa tgaatgagaa tataggagaa attgaatata cagaaaaaga aagactaaat
     8281 ttaggtgcaa attttaatgt ggatacagac gagaaatcta ttattggtgg agcaacggag
     8341 atacacctaa tacaaaaaga taataaactt gatgatgata ttataaatga taaagacgat
     8401 agaattaata acaaagaaaa tgaaatagag gaagaagaaa aattagataa tatacaactt
     8461 gaggctagaa tggttggaaa cattatcaaa gatttaatga aagtcaatga agatggaaaa
     8521 attcaaaagg tctatgataa gggaatagat ggatatagac ctgtggaatt tagagatata
     8581 gttattcttt taagagctac atctgcatgg gctccagtgt ttgcagatga attgatgaat
     8641 atggatatac caacgtatgc tgatgttgga gtggggtatt ttgatacgat tgaaataaaa
     8701 acaatattgt cattacttca aataatagat aatccaatgc aagatatacc tcttatatct
     8761 gtattaaaat cacctatatt tggatttaca ccagaggatt tgattgatat tagagttcag
     8821 agtaaagata aaatttttta tgaggtttta aaaagtacag cagagtatga tggatttaca
     8881 gattctcaaa atgaaaatga gtctgagttt ataccaagtg aagaatgcat aaacaaaagt
     8941 aaagattttt taattaagct aaaggaattt aaagaaaagt caatgtacat gagtacagat
     9001 gaatttatat ggtacttgta cacaaggact ggatactatg cctatgttgg agcattgcca
     9061 ggtggaagtc aaagacaagc taatttaaaa gtattgtttg agagagcaaa acaatttgaa
     9121 gaaaccagtc ttaaaggaat attcaacttt gtaaatttta tagagaaatt aaaaaaatct
     9181 agttctgata tgggaagtgc aaaaacactt ggtgagaatg caaatgttgt tagaataatg
     9241 agtattcata aaagtaaagg tctagaattt ccagtagtca tatgctctgc aatgggtaaa
     9301 aatttcaata ctcaagattt taaaaagagt attttatatc atcataattt agggtatgga
     9361 cctcaatttg tagactatga gagaaggatt tcttttccaa gtatagccaa agaagcatta
     9421 aaaagcaaaa taaatataga aaatttatca gaagagatga gagttttata tgttgcattt
     9481 acaagagcta aagaaaaatt gattataact ggttctacta gaaatataca agatagtata
     9541 aagagatggt caaatggaat tgaaagttta gatacaatat cacaatacga gatattaaaa
     9601 ggtaaaaatt ttttagattg gataatgcct tgtgtattaa gacataggga tttatctaat
     9661 ctactagaag aagtaggatt agacgcagtt tttaatgttg aacataattc aaaatggtat
     9721 ggaaagttgt ggaataagag tgatatttta gtggaaaaga agagtgatga agaaaaagaa
     9781 agtattgaag aaattcttga aaaaatagat gtagataatc ctgatagtga ttattacagt
     9841 gaaatagaag aaaaattaaa ctatatatat ccatatgaat ttagtacaag aaaacctgct
     9901 actatatctg ttacagaaat aaaaaagata caaaataatt atgaagaaga gttaataaat
     9961 actatatttg aacaaaaagt aatattaaaa aagcctctat ttattcaaaa tgaagaagaa
    10021 agagaaaaaa taagtggaac agaaagaggt acaatagtcc atttagttat ggaggtttta
    10081 gacttaaaaa atgtaagtag tgtaaatgat ataaaatcac aaataagagg ttttgtttca
    10141 aaaggtataa taacagaaaa acaagctagt atagtaaatc catataaaat atataaattt
    10201 tttgcctcta acataggaaa aagaatgttg aatgctgaaa ttataaatag agaaaaatca
    10261 atttatgcac aagtaaatat gaaagacata tatatttatg aaaagcttat aaacaatgac
    10321 gataaaaaac tttatgataa tgaaagtgtc atgttaagag gtatagttga tgcttatttt
    10381 gaagaagaca atcaaatagt tttggtggat tataaaacag attttgtaaa tgaggaaaat
    10441 ataaatcaaa taatagagaa atataaaaaa cagttggatt tatatgcaga tattattgaa
    10501 actttaactg gtaagtcagt aaaagagaaa tgtatatatt tatttggagt tgatgaagct
    10561 gtttgttatt agtttttata tataaattaa acctaaaaga tagaggaagg ggaaaaaatg
    10621 agatttattc atacatcaga ttggcatcta gggaagtccc tagaaggcca ttctagaatt
    10681 gaggaacaag caaagttttg tgaggaattt ataaaaatag tagaatcaaa tgaaatagat
    10741 atggttataa ttgctggaga tgtatatgat acttctaatc caccagcaca agcagaaaaa
    10801 ctattttatc agactgtatc aagacttgcc aataatggtc agaggtgtgt attaataatt
    10861 tcaggaaatc atgataatcc agagagactc tctgcaataa cacctctagc acatgaacag
    10921 gggatactta tatatggtta tccattaagt gctacgatag aagctaagta taaagggttt
    10981 gaaataacat atgcaactca gggatgtaca aaactgaata taaatggaga aaatattgtg
    11041 gttgctacat taccttatcc aagtgaaaaa agattaaatg aagttttttc aagtgaagat
    11101 gagtttgaga aacaaaaaaa ttattctgaa aaggtaggag atatatttag aagtttagaa
    11161 gaaaatttta gaagtgatac tataaatata gccgtatcac atatatttgt aataggtgga
    11221 gagagtactg aatcagaaag acctatacaa ttaggtggaa gttttttagt tgaaagaaaa
    11281 gatttgccag aaaaggctca atatactgca ctaggtcatt tacataaaca acaaaaagct
    11341 tccgaacgtt taaatgcata ctattctggt tctccacttc aatatagcaa ggatgaaaga
    11401 gcgtatacca aaggggcata catagtggat ataaaagctg gtgaaaaacc aataattgaa
    11461 gatgtatatt ttaataatta caaacctata gaagtattta aatgtaatgg aatagaagaa
    11521 gcactagata tatgtgagca aaatcaagat agagagattt ggtcatattt tgaaataaat
    11581 actgatgaga taatatcaca gaatgaaatt aaaaaaatga aggaactatt aaaagacata
    11641 atagaaataa aaccaataat aacatcttgt tatgaacaag aaagtgtaga tataaaagaa
    11701 aagtctatgg cagagttgtt tagagagttc tattctttta gtaaaggtgt agagccaaaa
    11761 ggagaactta tggatttatt tctagatata ataagtgaag aaggtgaatc agcagatgag
    11821 accaattaga ttagaactta cagggcttaa tagctatatt gataaacaag ttatagactt
    11881 tgaaaaatta attgaaagag gtttgtttgg gatatttgga actactggaa gtggaaaatc
    11941 tacaatctta gatgcaataa ctattgctat gtatggaaat atatccagaa atacaaaaga
    12001 atatataaac agtgtgtgcg ataaggcaat tatatcatac gagtttgaga taggaagcaa
    12061 aaacactaga agaagataca ttgtagatag aaccattgct agaagcaaaa caggaacaaa
    12121 gacctctaat gctagattaa tagaagtatt gaatgacaat acccaaaatg ttttagctga
    12181 taaagttgtg gaagtaaatg aaaaagtagc gcaggtcgta ggattaacgg caaatgattt
    12241 tactaggtca gtagttttac cacaagacaa atttaatgag tttctaagat tatcacgtgc
    12301 agatagaaga gacatgttag agagaatttt taatcttgag aaatatggaa gaagtcttgg
    12361 cgaaaaagtg aaaaagagaa aaaatataca gttacaaaat ttaaaagatt tgaaatcaaa
    12421 attaagtcaa tatgatggaa ttacagagga agtatataac aatataaacc aagaattaat
    12481 agagttaaaa aatttggaaa aagataagaa taatgcttta gacctagctc aaaaatctta
    12541 tgaagaaagt aaaacagtat atgaagaaca attaaagtta gaaaaaaatg aacttagaaa
    12601 aaatgagctt gatttaaaaa atagtgagat aaaagaaaag attgctcttg tagaaaatga
    12661 tagtaacgct aaaaaggtag acccatacat aagttcagtt caaaatctag aaaaaaagat
    12721 agatgaagat agcttcgcag taagtaattt agaaaagaaa ttagcaatat taaatcagga
    12781 gcttgaagtt acaaaaaata gatatgaaaa aataaacaaa attaagaatg aagaaatccc
    12841 taaattgagt gaagaaaaaa taagattgca acaagcaata aaattagaag aagagttagt
    12901 tttattagat agagagttaa aagagctaaa agaaagtgga attaatttaa ataagactaa
    12961 agttgaatta gaaaaagtaa agcaggtgtc agagtccaga aaagatgcag ttactaaatc
    13021 tataaaagaa gttgaaggaa agattgataa agtaaatata agtgcagaat taaaacaaaa
    13081 aatatttcta gcatatgaat acgagaaaga ttacaacaac gtattggaag aaaaaaatca
    13141 aaagctaaat aaactagaag aaatactcaa agagacagaa aatataaatt taaaagttag
    13201 atatattgat aaagataaga atgatgtaaa tcgtaattta gaaaatttat cacttcactt
    13261 ggatgttctt cttaaaaaat gtcctggtaa gagtgctgat ttacttttaa aatctgaata
    13321 tgttactgaa ttaagaaata aagcaaataa tactaaagaa aatgaaatta agaaaagcag
    13381 tatacaagat gaattaaaga taatacttga aagtaagttt aatactgaaa gggaagtaaa
    13441 tttactaaat gaaaaacttg aaaataatag aaagaacaga gatgacttag aaaaagaatt
    13501 agaagaacta aagtatttaa atttagcctc tgaacttaga cgtgaattaa aagaaaatat
    13561 gccttgtcca gtttgtgggt caaaacatca tgaaaatcat gatataacta agtatgatga
    13621 aaatatatct tttgtcaaag aaaaactgga gaaattagaa aaagaaaaaa taagtataag
    13681 aaacaatata gaggaattaa atgctaaagt aagtgggtat ttatctatag aaaaaatgaa
    13741 gactaaagaa cttgaagatg taaaaggaaa actagggggg attccatcta gtcaattatt
    13801 aaaaaaactt gatgaagaac aaagaaaact tgcattgtta aaatctaata ttcaggagtg
    13861 ggagaaagaa aaagagagta ctgaaaataa agtaacatta gctaaagaag aaaaaaataa
    13921 aattgaaaaa gaagaattaa aaataagaga gagtctgaat aattataaga aattaacaag
    13981 agatttaaac atagaaatag aaggtttaga aaataaatgt aaaaaactta aacaggaata
    14041 cctaggatta aagacgatta ctaaagtctc taatctattg tcaaaagtaa aagaaataag
    14101 ggagaatgaa aaagaattag aattactaag tactgggcat tcaaatttat taaaaaatag
    14161 agactcacta gatattaaaa taagagaaaa tgagagtaaa ctacatgaaa tagagataga
    14221 attaataaag gctagagaat tatatgtaga aaagaaattg agtagagata ataagtataa
    14281 agaagttatt agcataacga aaggtgattt agctaaaaat cttttacata atgttgaaga
    14341 aaatatatgt aagatattag aacaagaaga atcaagtaag aaaaaattgg aagagcaaag
    14401 attagaatat gaaaaaaaca ttgctgaaaa gcacaatata gatggtagac ttaaaactgc
    14461 taaggaacaa tataaagacc aaaaatcaac actaaataaa ctattggcag aaaataaatt
    14521 tgaaagtata tatgcagtaa aaagagcttt attagatact gatgcagtaa aaaaattgat
    14581 tgaagaaata ctagagcatg aagaagaaca aaagttatta tcatttaaaa taaaatcatc
    14641 aaaagaaaaa ttaaatggaa gaagcataaa gaaggagtac tttgaacagt taaaagatga
    14701 aatttataat ttgaaagtag aaataggaaa tatatccaaa gatattggag ctaatcaaaa
    14761 tcagttaata acattaaaag actctttaga taagataaat gattttaata aacaacttaa
    14821 ggtagttgaa cacaatgtgg acttattgga agaattagat aaatgtgtac aaggaaataa
    14881 atttgtagaa tatgtttcta caaaccaatt aaaatatata gcgttggaag catcaaaacg
    14941 attggatgga ataacaaaag gaagatatgc acttgaaatt gactcaacac taaactttgt
    15001 aatgagagat aactttaatg gaggagaaag aagaagcatt gatactctat ctggtggaga
    15061 gacattcttg acatctctat cattagcact tgctctttca tctcaaatac aattaaaggg
    15121 aagtgcacca ttagaatttt tctttttaga tgaaggtttt ggttcattag atagtgaact
    15181 tttagaaatt gttatagaat ccttggaaag attgcatagt aataatctaa gtgtgggtat
    15241 aataagtcat gtcgaggaac ttaaaaatag agttcctgtt aagctattag tttcatctag
    15301 tgaggctgga ataggttcaa aagtaaaaat tgaatatagt tagaaaatat atgttaattg
    15361 aatatattat ctaatatttc caaataatat gtaattgtga tataataaaa actaatatta
    15421 aagttattta taattaatat taatataaaa acatgtaagt aggagtgtgg taagtgttgg
    15481 aatcgcccaa taatttgatt cagattattt ttttaatagt attacttata gggtctgcat
    15541 ttttctcagc atctgaaacg gccttaatgt cgttgagtaa aatcagaatc agatatatgc
    15601 aagatgaagg agttaaagga gctaagttag taagttcatt aatagaaaat ccaaataagc
    15661 ttttaagttc tatattggtt ggaaataacg tcgtaaatat agcagcaaca tccatatcaa
    15721 catctttgtt tataggatta atgggagaaa aaggagtggc acttgctaca gctgttatga
    15781 cagtattagt attaatattt ggtgagatta ctccaaaaac aatagcagca aataattcag
    15841 aaaaggtttc tctattagta tctaaaccta taaaagcaat tatatttata ttaagaccaa
    15901 tagtatggat atttaatata attacaaata ttatatttaa attatttggt ataacaaata
    15961 aaggagctaa atcattcata actgaagaag agctaaaaac tatggtaaat gtaagtcatg
    16021 aagaaggcgt ccttgaaatg gaagagagag aaatcataaa taatgttttt gaatttggag
    16081 atatgcaagc taaaaatgca atggtacaaa gaatagatat ggtagctata gatatggaag
    16141 atagttatga tgagataata caggtattta aaactgaaaa attaagtaga atgcctgtgt
    16201 atgaagaaac tatagatgat attgtaggaa tacttaatat aaaagatatt atatttttat
    16261 ctgatgaaga aatagagtca tttgatatta aaaattatat gagagaacct tttttcacgt
    16321 atgagttcaa aaagataaca caacttcttg aagagatgaa acttgaaaaa agtcagatgg
    16381 ctatagttgt agatgagtat ggtggaacat ctggactttt aactatagaa gatttagtag
    16441 aagtcattgt tggagatata gaggatgaat atgatgaaga agaggatgaa atacaagtta
    16501 taaaagaaga tgagtatatt gtagatggaa gtactaaaat tggtgatgtt aatgaactta
    16561 ttggtgtaaa cttggaatct gaggagtttg attccatagg aggttttata attggacacc
    16621 taagcagatt gccagaagaa aatgaagtaa ttgaagttga taatataaga ttttgtatag
    16681 aaagtataga aaaaaataga attaaaaaaa taagaatata tacataatgt aataatgttc
    16741 ttaaatttat tagaagtgta taaatataaa agatatcttt tatatttata cacttctttt
    16801 tattttgtat tcaaataaaa ctagtatgaa atggacaaaa taaaaatata aattagaaga
    16861 aaggagattt ttaagatgag catactagaa aataattttt tatacaaatt aaaaaacaac
    16921 actatctttt tttgtatata tacattgtta tttattttta tttacaaagc gtttccatac
    16981 atagctccat tttttctagg tgctataata gcacttatga ttaatcctat atcacaaaag
    17041 ttagaaaata agtttcatat aaataaaggt atctctactc ttgttctaag ttttcttgca
    17101 gtggctatag tcagtacagt tactacaata atagtaataa attcaatgaa agaattaatg
    17161 ggatttttaa ataatatatc tgctaatcct gaggatataa gtaatactat tatgtatttt
    17221 ttaaataaaa taaatgactt tatgaaatca tttcaagaaa tagccaattt tgatgtagaa
    17281 caattagtaa acaaattcag tggtgaagta atgcaaatta caaaaaatct tcttactagt
    17341 atacttggat tggctacttc tattccatat attataatat ttataattac cttgtttatt
    17401 gctacgtatt ttatagcaaa agaccttgat aagattgaaa atagttttta taatatgttt
    17461 actgttgatg ttagaaagaa ggtaaaaaac gtaaagaaag aggcagggct ttcacttgtt
    17521 ggatatataa gggcctatac aatacttatg gctataacat tttttgcaat ctggggaagt
    17581 tttgctttat ttggattgaa atacggtcta atagtagggt ttgtaggagc tttaatggat
    17641 ttaattccat tcctaggtat aataacaata tatcttccag taattgttta ttattttctt
    17701 ataaaaaatt attttatagc tataagtatg actgtgatat tttttgtttt gtctttgatt
    17761 agagaaatac ttgaacctaa actagtatca gtaaatgttg gactaaatcc attagctacc
    17821 ctagcagcga tttttattgg aatccaagta aaaggaataa ttggagtaat tttttgtttg
    17881 ggtttggtat gtatgcatga tatacttaag aaggtggata ttttttaatt ataataattg
    17941 atagattttt taaaaataaa attttattca caggtaaata aaaaaatatt aatcttatta
    18001 agaaagaggg tatatacgtg aaacaaaaag tagtggaaag atttttaaaa tatgtatcat
    18061 ttgatacaac atctaattct cagtgtgaga actgtccatc ttcagaaggt caaagagttt
    18121 tagctaagta tatagttgaa gagctaaaaa caatgggtgt tgatgatgtg agtttagatg
    18181 aaaatagtta tataatggct actttaaaag gaaatactga tggtgtggat actataggat
    18241 tcatatcaca tttagataca attgaagatg ttagtggaaa agatattaaa ccaaaaataa
    18301 tagaaaatta tgacggaaaa gacatagttt taaatgaagc gttaaatgtt attacgtatg
    18361 taaaggattc tccagagttg gaagaattta aaggtgacga tttaatagtc actgatggaa
    18421 caactctact tgggtctgat gataaagctg gtatagctga gatagtgact gcaatagagt
    18481 atctaataaa tcatccagaa attaaacatg gagatataaa aataggattt acaccagatg
    18541 aagaaattgg tagaggtgct gatttatttg atgttgaaaa atttggagca aaatatgcat
    18601 atactttaga tggaggaata gttggagaac ttgaatgtga aaattttaat gctgcaaatg
    18661 caactattac tatacatggt agaaatgttc atccaggttc agctaaaaat aaaatggtaa
    18721 atgctataca tatagctgct gaaatatcag aaatgttccc agcagatgaa agaccagaaa
    18781 caacagaagg atatgaagga ttttggcatt taaattctat aggtggaaat gtcgagaatg
    18841 taagtatggc ttatattatt agagaccatt gtaaagaaaa atttgaaaat agaaaatcta
    18901 ttatgataga gaatatagaa aaaataaata agaaatacga taatagagtg gaattggatt
    18961 taaaagactc ttattacaat atgaaagaaa agattgagcc agttatgttt atagttgata
    19021 tagctaaaga agctatggag gaattaggta taaaaccacg tttagttcct gtaagaggtg
    19081 gtactgatgg agcaagatta tcttttaatg gattaccttg tccaaatata tttactggtg
    19141 gattaaactt ccatgggaaa aatgagtgta taccagttag ttctatggag aaagctacta
    19201 aactaattgt aagaatagct gaaaaatatg ctgaaagagt ttagttaaaa agtatttaaa
    19261 aattaagcat ttaaagttat aatagctata tcattcttta taagtcaagc tgattataag
    19321 cttgtgttgt acttaaaatg gtatagctat ttttgtaatt atttttataa aagataatta
    19381 tttttagttt agggtatcaa taaaaagtat gaatatcaat aggtattaac atttaaaagg
    19441 gggaattgta ttgaaaatta aatctaaaaa aataatagca tttgcacttt cacttgggat
    19501 tatgttgcca agttgtgtat atgcagacaa tattagtaaa aatcaaaatg cacaggtaga
    19561 tatgaacaat tatcttcaag gtagaaaatc tccaaaggag gaaaaactta ttggtgaaga
    19621 tagatttgat acagcgataa aaatatctca aagtggttgg aataatggtt cagaaagggt
    19681 gtttctagtt aactcaaatt cattgccaga tgcattggct tcaactcctt tggcaagtaa
    19741 attggatgca ccaatactgc ttacaaataa aaatagtata ccatataatg taacagagga
    19801 aataaaaagg cttaatccag cagaagttat tttaattgga agtgaaggag ctatttcatc
    19861 aagtgtaaaa gaatctcttg aagatatgga agtattagtc agtagaatcg gtggagcaga
    19921 tagagaagaa acatctttac ttcttactag acagctagat gatactggtg atttaggtgt
    19981 ctcaaaagtg gcagtggtta atgggtataa tggactggca gatgcaactt caatatcaag
    20041 cccagctgca agtgataata cagctataat atatacaggt aaggatagca taagaagtga
    20101 ggcaaagaat tttattacac aaaactcaac taagacatac atagtaggtg gggagtacag
    20161 tataagtaaa aaacttgaag gacagttagt aaactcagaa agactagcag ggacagatag
    20221 aaaagatact aatgcgaaag ttttagaaaa gttttatgga aaatcaagta aggttaataa
    20281 tatgtatttt gcaaaagatg gaagtggtag agaagctgat ttagttgatg ggcttgctgc
    20341 tggagtatta gcatcaaaaa caaagtcacc tgtagtactt gcaagtggaa gtttaagtag
    20401 tgcacagaaa agttttataa aaaaagtaaa ggctgataaa tttgtacaag ttggtggagg
    20461 taaaaatagt aaaccatatg cagaagcatt agccttacag taatattgtg taaatcaaaa
    20521 ttaatatata ataatagcaa gtaaaccaag ttgcacttta atatatataa taaaataagt
    20581 aaactaagtt atatttcagt ataatatata ataaaataag taaactaagt tatatttcag
    20641 tataatatat aatgaaataa gtaaactaag ttatatttca gtataatata taatgaaata
    20701 agtaaactaa attatactcc aatataatat ataatagaaa caagtaaact aagttatatt
    20761 tcaatatagc atataataaa acaagtaaac cagattctat ttaattagaa gtttaaggta
    20821 ttgtacttaa acttctaatt gagatagaaa taaaatggtt tacttgtctt tattatgaat
    20881 ttaataaatt aaagcttatt ttatttttat taaaaattta ctaatcattg atatttatta
    20941 tatttccagt attgatgtca attaagttta tatcaagttc aagctcatcc tcatcatcag
    21001 tttttatgat atctataatt gcgacagaat ggcttccatc ttttgggata gaagtacttc
    21061 ctggatttat tactattaaa ttatcatcca tgtatagttt ttttacatga gtatgtccta
    21121 aaactaatat gtcaccaccc attttctttg ctttgtcaat aatttcttct tctgattcta
    21181 tataaccatg attcaatata attctaattt caccaaattg gctcatgaca taaggactct
    21241 gaataggatg ttctataacc atctggtcaa catctgcatc acagttccct tttacaatca
    21301 caatgttttc taatttattt aatgtctcaa taaatttttt aggattatat ccttctggaa
    21361 tatcatttct aggtccatga tataagacat ctccaccatg taataaaaca tcacaatcag
    21421 ataatacatt taacgctttt tcaaagtaaa gtaagctacc atgagtatca ctcattattc
    21481 ctattttcat attaaaaatc ctcctaagta cagtctataa aaatttttca gatactctat
    21541 gccaaaactc atagtttgac attctaagta attttatttt tgcatcagat atactaactt
    21601 ttatattttc gatttgtcta aatctgtgct caactccatc aacgacaatc aatacagaat
    21661 cttcaaatct atattctggt gcaatcttta taacagattc atttgaacat attatacttg
    21721 atgtaaagca cctatatgca ttggtattta tcggatgtaa aggtgtaatc tgcataagtt
    21781 caagatttat atcaacaata ctacctccag cagaatagtt ataggctgtt gaacctgtgg
    21841 aagtagaaat aatcatacca tctccactaa aattttgtat atgtttatta tcaaggctta
    21901 aatttaggtg tatagtccta gacttgtctc ctcttataac aacctcattt acagcaagca
    21961 tattagaacc actagtagtt gtatatactt ccgcattaag aagtgacatt tcttgaataa
    22021 tataatcttt ttttgtataa gcttcaataa agctatcaat cttatctggt aaaatatcag
    22081 gaaaaaaccc cagatgacct gtatttattc ccattatggg aatctcagga aaatcgaaat
    22141 ctctaactgt tcttaaaaaa gagccatcac caccaattga tataataagc tctgtgtctg
    22201 gataaatatc tgaatacact tcaaagccag cattgataag ttttctagtt aagagttctt
    22261 tagtctcaag ggacttattt aattggtttg tatttatagt tataattctt ttcatatatt
    22321 cacctcttca tactttgtat tataacatag ttttataatt cttaatttat aaatttataa
    22381 atgacatttg tagtgaatgt gttaaaatat gtataggtgg gtataaaaaa atgcataaga
    22441 ggtgaagatg tgtttagcat aaggaatatc acaaaaaaat taggaaaatt caagttaaat
    22501 aatataaact tagagttaaa ggaaggggat attgttggga ttataggacc aaatggttcg
    22561 ggaaaaacga ctttaataaa aattataatg gggattattg atgcagatga aggtgaaatt
    22621 gaactttgta atgaaactat tgaaaattct ccaataagct ttaaaaacaa tattggtttt
    22681 gtatatgatt ctttacaatt ttatccgcat ttaaaggtta aggaatttag aaaaatagtt
    22741 tctttatttt ataagaattt tgatagagaa aggtttgatg aataccttaa taagtttgat
    22801 atagaagaaa atatgcatat tgaaaattta tctaaaggtc aatctgaaaa gcttatgtta
    22861 tcaagtgcac ttagtcacaa tgctaagtta ttgattttgg atgaaccaac tgctgggata
    22921 gacccaattg taagaactga aataatgcag tacttacaag attttgtgaa aaatggaagt
    22981 agtagtgtaa taatttctac tcataatact gataatttaa ttaaaattgc tgattatctt
    23041 gtttttataa atagaggaaa tcagattttt actgttaaga aagaattaat agaacaagaa
    23101 tataagataa ttagggcaaa taaagcagag ttagaagcaa ttaaagaaag catagttggt
    23161 gtgaaagagt ataaatatta taatgaggca cttgttaaag ttggagatag cttgaaagta
    23221 aaaagcctat tgatagaaat tgataagcat aaggtcaaaa atcctactat agaagaacta
    23281 atgtattatt atgtaaatga ggtgagatga tgttcaattt aataagaaaa gatttaataa
    23341 tagggattag ttcagatggt atccgtaatt taaagtacat attgctattt tttgtgtttt
    23401 attttttcct taattcaatt tcatattata cagtaagtat tgttgttagt tatttgatat
    23461 ttataaatac gtttgagtgt gattatgaaa atgattcaag gatttttata aggagtatgc
    23521 cagttagtat agaagatata gtttactcaa aatatttatt aggtgtagga ttaataattt
    23581 ctgtaacaat aattgtgtcc ttactaagta aattgacatc actagtgttc tttagaaata
    23641 tggttttaaa tgatgtattt ttttctgtaa atatattttt agcaattttg acgattttac
    23701 tgcctttaat ttttaagttt ggatatggaa aaatgaaagt ttgtggtttt atagtaagta
    23761 ttttaattta ctttgtatat ggaagtcttt tgaagatgat tagtatgatt gtatatcaag
    23821 taaagcattt taattatagt aaagttggag gagtatatct ttcaaattat gtaactgata
    23881 tagctaatac taagtatata aatttatata gtatgacatt tttaacaata ataattttta
    23941 taatatcaat gtatttttct attaagattt caaaaaagaa taagtttaat tattaaaatt
    24001 ttgattaata ttatagcaca atagcacaaa atataaggag actaaaaatg aagaagttta
    24061 taaaagtttc attttttatt ttggggattt ttacactatt catagctata aataaattag
    24121 tatatagaga tatggtaagt gtcaaagatt atgaaataaa tcatttagag gaaattgttt
    24181 ttgattttga tagcaggaga agtagagatg ttggtactat tgtaagattt aaaataagca
    24241 ataatagtaa atatagttat aaattaaaca gtgctaaaat gaagtttgaa aattgtgtgg
    24301 aaaataaaaa gggaatgaat aagtgttctg catatgtaaa tgtagattta tttaatgaaa
    24361 aaaattctat aactgaaaga actctgaaat atggaataaa accaaataat caaggttatg
    24421 ttgattttgt aattccaaaa ggactaaatt ttgattatag gtactttaat gaatctggta
    24481 tgagtgtaga gtataaagga gaatatattg tcaatattcc aatggcaaaa ggactttatc
    24541 tagtagttgg aaaaaataat gatacatgga atgctaatat catggaataa tttgaaaaat
    24601 aaatcaatta tagatataat taagaataat atgaaattta taaaagttca gactaggagg
    24661 gatatttaat gtcaataagt aatgtaagag agtattttaa acaatttggc aaggaagaca
    24721 gtatattaga atttgaacaa tctagtgcta ctgtggaact tgcagctgaa gcagctgggg
    24781 ttattccagc aagaatagct aagacactct catttaaaat aggagatgat gcaattttaa
    24841 ttgtgacagc tggtgatgct aagattgata ataagaagta taaggctgaa tttaattgta
    24901 aggctaaaat gttgacacca gaagaagtct tggagtttac tggacatgct attggtggag
    24961 tctgtccatt tggtttaaaa aattctataa aagtctattt agatgattca atgaaaagat
    25021 ttgatacagt atttcctgca tgtggcagta gtaactctgc aatagaactt acatgtgaag
    25081 aaatggagaa gttttctaag tgtgaaaaat gggtagatgt ttgtaagaat tggtaaaaaa
    25141 atatagtaaa ctatagtagg aattagtaaa aaactacctc tgtatacaat ctatacagag
    25201 gtagtttttt tgtgtaaata tttataaatc taagaggaaa atctaattta aactaataat
    25261 ccaaaataaa gttaaaaaat tctgattttt tttcgaaaca ataaaaaaat gacaaaaaag
    25321 tggaaaaaat gtaataaata ttgttaaaaa aataattaga aattattgaa aaattatcct
    25381 ttgagtgata ctattataga tgtaagatat ttaaaaataa aaaatagtca gataaaaata
    25441 cgattattta gctctattaa aataattttt atacgataaa aatcttctgt acatggctaa
    25501 aaaacagatg tacagacaat atttaatgtt taatataccc taatataaat atcgtaaaca
    25561 aattatttta tctgtaacca gtgtgaaaga aatcttattc taacttgaaa tttcacaaaa
    25621 ttccaaaaag cgagattttt tttaagtata gttgatttta aattaacaat ttatgattaa
    25681 attaatgtca taattggtta gtataatcac atataaatat agagcgtagt ttgattaagg
    25741 ttataaattt ctcgacgtat attcaaatac cttattatag atgttgccga gattttaatt
    25801 aataaatgaa aataaaatat ttataaaata ctgaggaggc tattatggat ttaaattcta
    25861 aaaaatatca gatgcttaaa gagctatatg taagcttcgc tgaaaatgaa gttaaacctt
    25921 tagcaacaga acttgatgaa gaagaaagat ttccttatga aacagtggaa aaaatggcaa
    25981 aagcaggaat gatgggtata ccatatccaa aagaatatgg tggagaaggt ggagacactg
    26041 taggatatat aatggcagtt gaagaattgt ctagagtttg tggtactaca ggagttatat
    26101 tatcagctca tacatctctt ggctcatggc ctatatatca atatggtaat gaagaacaaa
    26161 aacaaaaatt cttaagacca ctagcaagtg gagaaaaatt aggagcattt ggtcttactg
    26221 agcctaatgc tggtacagat gcgtctggcc aacaaacaac tgctgtttta gacggggatg
    26281 aatacatact taatggctca aaaatattta taacaaacgc aatagctggt gacatatatg
    26341 tagtaatggc aatgactgat aaatctaagg ggaacaaagg aatatcagca tttatagttg
    26401 aaaaaggaac tcctgggttt agctttggag ttaaagaaaa gaaaatgggt ataagaggtt
    26461 cagctacgag tgaattaata tttgaggatt gcagaatacc taaagaaaat ttacttggaa
    26521 aagaaggtca aggatttaag atagcaatgt ctactcttga tggtggtaga attggtatag
    26581 ctgcacaagc tttaggttta gcacaaggtg ctcttgatga aactgttaaa tatgtaaaag
    26641 aaagagtaca atttggtaga ccattatcaa aattccaaaa tacacaattc caattagctg
    26701 atatggaagt taaggtacaa gcggctagac accttgtata tcaagcagct ataaataaag
    26761 acttaggaaa accttatgga gtagaagcag caatggcaaa attatttgca gctgaaacag
    26821 ctatggaagt tactacaaaa gctgtacaac ttcatggagg atatggatac actcgtgact
    26881 atccagtaga aagaatgatg agagatgcta agataactga aatatatgaa ggaactagtg
    26941 aagttcaaag aatggttatt tcaggaaaat tattaaaata gataaatatt aaaatataca
    27001 caataggtcg atattaaaat agatatggag gaaggattta tgaatatagt cgtttgtata
    27061 aaacaagttc cagatacaac agaagttaaa ctagatccta atacaggtac tttaattaga
    27121 gatggagtac caagtataat aaaccctgat gataaagcag gtttagaaga agctataaaa
    27181 ttaaaagaag aaatgggtgc tcatgtaact gttataacaa tgggacctcc tcaagcagat
    27241 atggctttaa aagaagcttt agcaatgggt gcagatagag gtatattatt aacagataga
    27301 gcatttgcag gtgctgatac ttgggcaact tcatcagcat tagcaggagc attaaaaaat
    27361 atagattttg atattataat agctggaaga caggcgatag atggagatac tgcacaagtt
    27421 ggacctcaaa tagctgaaca tttaaatctt ccatcaataa catatgctga agaaataaaa
    27481 actgaaggtg aatatgtatt agtaaaaaga caatttgaag attgttgcca tgacttaaaa
    27541 gttaaaatgc catgccttat aacaactctt aaagatatga acacaccaag atacatgaaa
    27601 gttggaagaa tatatgatgc tttcgaaaat gatgtagtag aaacatggac tgtaaaagat
    27661 atagaagttg acccttctaa tttaggtctt aaaggttctc caactagtgt atttaaatca
    27721 tttacaaaat cagttaaacc agctggtaca atatacaatg aagatgcgaa aacatcagct
    27781 ggaattatca tagataaatt aaaagagaag tatatcatat aatttaaggg gggataaaga
    27841 atgggtaacg ttttagtagt aatagaacaa agagaaaatg taattcaaac tgtttcttta
    27901 gaattactag gaaaggctac agaaatagca aaagattatg atacaaaagt ttctgcatta
    27961 cttttaggta gtaaggtaga aggtttaata gatacattag cacactatgg tgcagatgag
    28021 gtaatagtag tagatgatga agctttagca gtgtatacaa ctgaaccata tacaaaagca
    28081 gcttatgaag caataaaagc agctgaccct atagttgtat tatttggtgc aacttcaata
    28141 ggtagagatt tagcgcctag agtttctgct agaatacata caggtcttac tgctgactgt
    28201 acaggtcttg cagtagctga agatacaaaa ttattattaa tgacaagacc tgcctttggt
    28261 ggaaatataa tggcaacaat agtttgtaaa gatttcagac ctcaaatgtc tacagttaga
    28321 ccaggggtta tgaagaaaaa tgaacctgat gaaactaaag aagctgtaat taaccgtttc
    28381 aaggtagaat ttaatgatgc tgataaatta gttcaagttg tacaagtaat aaaagaagct
    28441 aaaaaacaag ttaaaataga agatgctaag atattagttt ctgctggacg tggaatgggt
    28501 ggaaaagaaa acttagacat actttatgaa ttagctgaaa ttataggtgg agaagtttct
    28561 ggctctcgtg ccactataga tgcaggttgg ttagataaag caagacaagt tggtcaaact
    28621 ggtaaaactg taagaccaga cctttatata gcatgtggta tatctggagc aatacaacat
    28681 atagctggta tggaagatgc tgagtttata gttgctataa ataaaaatcc agaagctcca
    28741 atatttaaat atgctgatgt tggtatagtt ggagatgttc ataaagtgct tccagaactt
    28801 atcagtcagt taagtgttgc aaaagaaaaa ggtgaagttt tagctaacta atttcttggg
    28861 gagggtatta tgagtacaag tgatgttaaa gtttatgaga atgtagctgt tgaagtagat
    28921 ggaaatatat gtacagtgaa aatgaataga cctaaagccc ttaatgcaat aaattcaaag
    28981 actttagaag aactttatga agtatttgta gatattaata atgatgaaac tattgatgtt
    29041 gtaatattga caggggaagg aaaggcattt gtagctggag cagatattgc atacatgaaa
    29101 gatttagatg ctgtagctgc taaagatttt agtatcttag gagcaaaagc ttttggagaa
    29161 atagaaaata gtaaaaaagt agtgatagct gctgtaaacg gatttgcttt aggtggagga
    29221 tgtgaacttg caatggcatg tgatataaga attgcatctg ctaaagctaa atttggtcag
    29281 ccagaagtaa ctcttggaat aactccagga tatggaggaa ctcaaaggct tacaagattg
    29341 gttggaatgg caaaagcaaa agaattaatc tttacaggtc aagttataaa agctgatgaa
    29401 gctgaaaaaa tagggctagt aaatagagtc gttgagccag acattttaat agaagaagtt
    29461 gagaaattag ctaagataat agctaaaaat gctcagcttg cagttagata ctctaaagaa
    29521 gcaatacaac ttggtgctca aactgatata aatactggaa tagatataga atctaattta
    29581 tttggtcttt gtttttcaac taaagaccaa aaagaaggaa tgtcagcttt cgttgaaaag
    29641 agagaagcta actttataaa agggtaaaaa tctaattaaa aatatatttg ctgttaacta
    29701 gattaaatag tattttaatc tagttaacag aatataaata ctaaaataac aggaggttat
    29761 aactatgaaa ttagctgtaa taggtagtgg aactatggga agtggtattg tacaaacttt
    29821 tgcaagttgt ggacatgatg tatgtttaaa gagtagaact caaggtgcta tagataaatg
    29881 tttagcttta ttagataaaa atttaactaa gttagttact aagggaaaaa tggatgaagc
    29941 tacaaaagca gaaatattaa gtcatgttag ttcaactact aactatgaag atttaaaaga
    30001 tatggattta ataatagaag catctgtaga agacatgaat ataaagaaag atgttttcaa
    30061 gttactagat gaattatgta aagaagatac tatcttggca acaaatactt catcattatc
    30121 tataacagaa atagcttctt ctactaagag accagataaa gttataggaa tgcatttctt
    30181 taatccagtt cctatgatga aattagttga agttataagt ggtcagttaa catcaaaagt
    30241 tacttttgat acagtatttg aattatctaa gagtatcaat aaagtaccag tagatgtatc
    30301 tgaatctcct ggatttgtag taaatagaat acttatacct atgataaatg aagctgttgg
    30361 tatatatgca gatggtgttg caagtaaaga agaaatagat gaagctatga aattaggagc
    30421 aaaccatcca atgggaccac tagcattagg tgatttaatt ggattagatg ttgttttagc
    30481 tataatgaac gttttatata ctgagtttgg agatactaaa tatagacctc atccactttt
    30541 agctaaaatg gttagagcta atcaattagg aagaaaaact aagataggat tctatgatta
    30601 taataaataa gaatttggga attaaaagtt taaataaaat tgtttaaaaa acaatttcgg
    30661 atatatgaaa aatctaattt aatgggggta atgaatatga gagaagtagt aattgccagt
    30721 gcagctagaa cagcagtagg aagttttgga ggagcattta aatcagtttc agcggtagag
    30781 ttaggggtaa cagcagctaa agaagctata aaaagagcta acataactcc agatatgata
    30841 gatgaatctc ttttaggggg agtacttaca gcaggtcttg gacaaaatat agcaagacaa
    30901 atagcattag gagcaggaat accagtagaa aaaccagcta tgactataaa tatagtttgt
    30961 ggttctggat taagatctgt ttcaatggca tctcaactta tagcattagg tgatgctgat
    31021 ataatgttag ttggtggagc tgaaaacatg agtatgtctc cttatttagt accaagtgcg
    31081 agatatggtg caagaatggg tgatgctgct tttgttgatt caatgataaa agatggatta
    31141 tcagacatat ttaataacta tcacatgggt attactgctg aaaacatagc agagcaatgg
    31201 aatataacta gagaagaaca agatgaatta gctcttgcaa gtcaaaataa agctgaaaaa
    31261 gctcaagctg aaggaaaatt tgatgaagaa atagttcctg ttgttataaa aggaagaaaa
    31321 ggtgacactg tagtagataa agatgaatat attaagcctg gcactacaat ggagaaactt
    31381 gctaagttaa gacctgcatt taaaaaagat ggaacagtta ctgctggtaa tgcatcagga
    31441 ataaatgatg gtgctgctat gctagtagta atggctaaag aaaaagctga agaactagga
    31501 atagagcctc ttgcaactat agtttcttat ggaacagctg gtgttgaccc taaaataatg
    31561 ggatatggac cagttccagc aactaaaaaa gctttagaag ctgctaatat gactattgaa
    31621 gatatagatt tagttgaagc taatgaggca tttgctgccc aatctgtagc tgtaataaga
    31681 gacttaaata tagatatgaa caaagttaat gttaatggtg gagcaatagc tataggacat
    31741 ccaataggat gctcaggagc aagaatactt actacacttt tatatgaaat gaagagaaga
    31801 gatgctaaaa ctggtcttgc tacactttgt ataggtggag gaatgggaac tactttaata
    31861 gttaagagat agttttagat tataatattt acaaattaaa tatttaatta tgaatttaac
    31921 ctgtaagaat aactatttgt tattcttaca ggtttttata gttaaaaata attattatct
    31981 attaaacaaa aatagtgtaa aatatataat atcatgattt tgaagatata aaaaagtagg
    32041 aagatataaa agaaagtagg taaaagtttg tttaaaaaag agaatcaaag atataatctt
    32101 atatcgtata caaatgaaga agaaatgact ttaaaagaag ttttattgga caagctaaat
    32161 ttctcagtta ggtcattatc aaaaatgaag agggaaaaga gtgttttagt aaatggagta
    32221 tataaaaaac cgagtttaaa ggtgtatagt ggtgatttaa tagaagtaaa aatagacgag
    32281 gaaaaagcaa attttgaacc tcaagattta aatttacaga taatttatga tgattttgat
    32341 ataattatgg tgaataaacc tccgtttatg gtagttcacc caacaaaaag ccactatgat
    32401 aaaacaatag caaatggtat tagttactat atagataatc aaaaagaaaa tgtaaaaata
    32461 agatttgtaa ataggttaga tatgaatact tcagggttag ttatagtagc aaaaaatgct
    32521 tatgcacatc atactttgtc tacagcaatg agtgaaaata aagttgaaaa aaagtatata
    32581 acagtagttg atggaattat aaaagaaaat gagggaacta tagatgagcc aatttataga
    32641 cctacagaag attcaataaa gaggataata gatgaaagag gacagtcatc tgttactcac
    32701 tataaagtaa tagaaagact agaaaatgca actgtattag aagtaagctt agaaactggt
    32761 agaacacatc aaataagagt acatatggct catataggtc atggaataat aggtgatgag
    32821 ttgtatggtt atgtagatga agatctaata aataggcaag cacttcatgc atataagcta
    32881 gagtttgaac aaccaagaac aaaagaaaaa ttgaaattta aagcagatat accagaggat
    32941 atgaaagagt taatatcgaa attaagataa tggggtgata tatatgataa tacataaatt
    33001 tataatacat gttttggaca aaaatagtga tgttccaata ttaaatgact ttgaaggcaa
    33061 agttaatcaa gaagtagatg gttttttcca gaaagctata aaaagaattg caaaagatga
    33121 ggatttaaga aaaggtgttt ttaaagatta taatgacaat ttaattaaaa actgttgtga
    33181 acaaataata tatgatgaaa gtactttttt gaaaaattct aaagaaattg catcttactt
    33241 gtttgatgtt atgaaaatta atgctgcact agaatcttgt gatttggcaa tttgcttata
    33301 cactattaaa gatgaaaaga gtgtagcaat actgaaatta gattataaaa agttgtacac
    33361 tcattcaatt gaatttgtgg atgataaatt taatattcaa atggtttcaa atgaaattgg
    33421 tatcccagag actttaagac aaaagcaagg agctttgatt agtctaagtg gtataaatga
    33481 tgagtttcat ctaaggcttt tagataagga tgctgaaaaa gaagggtcag aatctaagtt
    33541 tgtaacagaa tttttaaatg ctaaaaaaat agatgatgat aagtacaaga ccaaggtatt
    33601 taaaaataca gctgaaaatt ggataaccaa tgctcttagt aatgatataa agcaagctga
    33661 agatgtaaga agtatattaa attatacact gaaggaaaaa catgaagttg atataaatga
    33721 ctttgttgat aattcaatta aagatgatga gttaaaggat agctttaaag agcatatgga
    33781 agaaaaaggt cttgacgaaa gttttagtat agataaaaaa tgggttgaga aaaagcttaa
    33841 aaaaagaagt ataaaaacgg ataatggttt tgacataaaa gggaatctaa ctgattttga
    33901 agacccaatg aagtatactg taaaacaaaa tcaagatgga actatagata taataataaa
    33961 gaatgttacg ttttatgaag aaaagtaatt tatatactta gtatataaat taaatataag
    34021 agcgtgtatt ttatatattg taaataaatg taatctagtc tgtgcgaact ctaatataat
    34081 ttattatgta gaagtttaca cagactattt atatttgtag acaaaacgat actatttgtt
    34141 ttcttcgata tatttacata tatcaccaat acatttaaag ttttcagctt cctcgtctgg
    34201 tatttctatt ccaaattcat cttcaagggc cattataatt tcaacagcat ctaatgaatc
    34261 agcttctaag tcgtccatta aagatgcttc catagttatc tcatctaaat tgtctacccc
    34321 taattgttct gctattattt cttttatttt ctcaaacatt ttaagcctcc taaagcagtt
    34381 ttaattaatt ttaatgttag tttaatataa tatcaagaat ttttcaatat ggaatctata
    34441 aaaaataata aaattttata aatgtagtaa taaagtatta tttgatgaat aaactttatt
    34501 gataacaaat ataacttagg aggaaatgct atgaaatatg taggactatt actaacttca
    34561 gtaggaatgt ttttacttat agcagtaaat ttttattata attcaataac tttagatatg
    34621 caacgtatag aggattatgt aatggagacc aatttaatcc ttgaagatgt agctgaaaag
    34681 gagagttatg tatctaatga aaaagaagat tacatatcaa ggttaatgca tgtaaagaaa
    34741 ggaatagaaa attcaaaaac atccttcttg atagaaagat ataaggagta caaaataaaa
    34801 tcaatagaaa gtttaatcta tactatatca gaggagaaaa aagattatct ggatgaagtt
    34861 gatagataca ataaacttgg tgaaaaagaa ataaataaac taatcaataa aaacttttta
    34921 gaagtaactt atctgtctat tactacatat atttaattaa agagacaaaa cttagtctct
    34981 taatgattca aggaggatta gttatgacaa aattagaaga caatatggaa cgtttctttg
    35041 gaggatgcga aggatttttt gggaataaaa tgttgataat aatagttata gtattcttac
    35101 tattatgcac agacatttta gaagacttat tatgcgatga caatatatgg atatgggtta
    35161 tattaatagt attgttatta tttaacttcg atgatggttg ttgttgctaa aaaatattga
    35221 ttattaaata ggtaaaacca gtaacttata caagttatgg ttttaccttt tttagttcaa
    35281 attaaaaaat ttaaaaaaat agtaaaaata cattagcaaa atggaaacca aatgcatagt
    35341 atatgttagg agcgaaaagc tcaacaaaat atcaacagga ggtaacgtat aatgttagat
    35401 agattctttg gagaagaagg atgttttggt ggaggaggat ggtggataat agtactattc
    35461 ttcctattct tagcttttgg agatagctgg gcagaatttg acataatggc atggatacca
    35521 ttcctaatag ttttattaat actttgctgc tgtggtggat gcttagaata agaacacagt
    35581 tattaaaaaa aggagggctt tgttatgtta gatagatttt ttggagaaga aggatgcttt
    35641 ggtggaggag gatggtggat aatagtacta ttcttcctat tcttagcttt tgaagattgc
    35701 tggagagaca ttgacataat gtcatggata cctttcttaa tattattgct aataacttgt
    35761 tcatgtggtg gattctttga tggtgaagta ggttgcggct gctaatactt aaatcattag
    35821 aataaaaaaa tgctggtatt ttataccagc atttttttat tttatttttt aatatgattt
    35881 aaattaagaa tgaatattat taaatttgtt gaatataggg tataatataa aatataatgt
    35941 atttaaatgt acttgctttt atggtaaaat aaaaacttat ataaatgtag ttaaaatagg
    36001 aaccttcaaa aatttggttc agttttagat aaatttagta aaataaggag gatgaagatg
    36061 aaaggcaata taacgataaa agatgttgct aaacaagcag gagtgtcaat atctactgta
    36121 tctagagtta taaatgattc aaaacctgta actgatgaag tcaaacaaaa agttttagag
    36181 gttataaaag agactggata tataccaaat ccacttgcta gaagcttagt aacaaagaag
    36241 agtcaattaa taggggtaat agttccagaa gtttcagatt cttttgttaa tgaggtgtta
    36301 aatgggatag aagaggttgc taaaatgtat gactatgata ttcttttagc gaatacatac
    36361 tctgataagg aacaagaact taagagtata aatctattga gagcaaaaca agtggaaggt
    36421 atagttatga tttcatggat agttgaacaa gaacatatca actatataca aaattgtgga
    36481 ataccagcga catatataag taaaactgct agaaattatg atatatatac agtaagtact
    36541 agcaacgaag aagctacttt tgatatgaca gagcatctta taaagaaagg tcatgaaaag
    36601 atagctttta taatgactag taaagatgat actgttttag aaatggaaag acttgctggt
    36661 tatgagaaag cactttcaaa taacaatata gaattagaca agagtttgat taagtatggt
    36721 ggaactgatt atgagagtgg atacaatagt atgaaagaac tattagatga tggaataata
    36781 cctcatgcgg cttttgtaac aggtgatgag gctgccatag gtgctataaa tgctatatgt
    36841 gatgctggat ataaggttcc agaagacata tcagttgcag gatttaatga tgttaagata
    36901 gctagaatgt atagacctaa acttactaca gtatatcaac ctctatacga tatgggagca
    36961 gtagcaataa gaatggttat aaaattaata aataaggaat taattgaaaa taagaaaata
    37021 gagttacctt atagaattgt tgatagagaa agtgttacag aaagaaaaaa ataaaactat
    37081 taaaatcaat caggtagtgc cttaggcact acctgatttt ttatgtcaat aatatcaaat
    37141 ttaataatag attaactaaa aataattatc taattgctta aatagtttat ttttatctat
    37201 tcatgaggat tagacttttt agttttctta aataaacttt tagatttttt ttgttgacta
    37261 cctggtgtat tttttggtgc aggtgaattt gacttacctt tagtaggttt agccttctta
    37321 gttttagctg gtttagtatc tctttggttt tctgcatctg agattttttc cattatatta
    37381 tccagttttt tgcgttgatt ttcatctaaa aatgctttga attttgatac tacttcatct
    37441 ctgccatcta gattcttacc tatttttata agttcctcca taagttcatt ttctgatttt
    37501 cctttgtaac tatcagccat cttttctatt ttatccttgt caatattttt ttgtttagcc
    37561 attttttcta atgactctgt atctaatttt ttcataaaaa aacccctttc tataaatata
    37621 tattgaatta tattcatact atttatatat agaaataata ttgaagtggt gaattgtgag
    37681 tgatatataa tataaataaa aattcttata gataatatat atgcactgag ctttaaaaat
    37741 ggaacattaa aatatgaaaa acatacttta atatagggca attatataat ataaggggga
    37801 attgtcttga ataacttact atttttgatt ggagtgattg cattgagtaa tggtaatatc
    37861 tctatatttg atgataaaaa taaatcaaaa ggaaataata gagttagaaa aaacaaaaaa
    37921 aatgcaaatg ataaactttc acagtctaca catggcaatt taaggtttga tgcaaatgat
    37981 ataaaaagag gtttaaggct catggattta agtgaagaag atttagaaat gggaatggaa
    38041 ataataacaa gaactaaaaa atacatgtca agagatgaaa gaaaaatact aataaaaata
    38101 gaaagtgttt tagatttagt tagaggaata aaaaaattaa ataatataga tatggtagat
    38161 acagaagatg aaacggattt tttcagaaaa atggatagtg atgataaaaa gaatatgatg
    38221 ataaaggaaa ttatagatgt attcccagaa aaaagaaaaa attcagttga aaaagctata
    38281 ggaatgaaaa agaagataga tttatttgca gaattatttt taccagatga ctttggtgaa
    38341 ggtggattta gtttgagctc tttggcaaat ataaacaatc ttggtagtat gaataatctc
    38401 aaattgttag gaagtctttt aagaggtgat gatagtaata atgatgatga agacaataat
    38461 gatgagttaa attatgaaga cgaagaatta gattgtgaag acaaagaatt ggatgatgat
    38521 gagttaacag atgaaggtga aatggaggat aatgaagatg aattagtaga agataagaac
    38581 acaggctatg aaaatataga aaatgatgaa gatatagtat acgatgagga actatacaac
    38641 attaatgagc aagaatccga atacaattat aatgaaaaaa atactaaaac tagagctaag
    38701 aaaaaacgat aattaactaa atacataaag aaaaaaggaa gaaaaagtaa tgatagaata
    38761 agattatctc aattactata taaaaccact ttaaaaaatt caaaatttat tatataataa
    38821 taatatgaaa tcctgaaaca tacgataagg cttttttaaa ttcaacctac aaatgaaata
    38881 cgggagaccg tgtctggtaa attataacat gcctagttag tttggacgtt aaaggttact
    38941 ggcaagtcac ccacctggga gtgcctaggg tgtgaattta cttagccacc ggtgtttcgg
    39001 gacaaaaaaa gtgctactat ttcaaatagt agtacttttt ttattaatat gcatttttct
    39061 actaaatacg cctaattatt gataaattca aaaagcaagt aacaattaag attataaaag
    39121 catagtatta attaggcttc aaaaagccaa agacttaata agaatcgctt acagggaggg
    39181 caatgaaaaa tgcaagatta taaaaaaaat aaaagaagaa tgatgaatca gccaatgtct
    39241 acaatgaatg aagaagaagt gtatacagat gaaataaatt cagaagacat gagaggtttt
    39301 aaaaaatcac accatcataa tggatgtaat actgataata agtgtgagtg ccatgatgat
    39361 tgcaatccat gcaacccatg taatccatgt aaacctaacc catgcaatcc atgcaaacct
    39421 aatccatgtg atgacaattg tggatgccat gacaattgta aatgtgattg tgaaccatgt
    39481 gaaatggatt cagatgaatg ttttgaaaac aaatgtggac cagaatgctg taatcctata
    39541 tctccaagaa acttctctgt atcaaatgca gtgccatttg caatagaggc taatagaata
    39601 tttgatacta tgcaattcca aacatttaca gatgcaacag gaccaaatgg agagccatta
    39661 acttttgaaa cagaagtagt agaagtattt ggttcagttc caagtgcagg tcaagcaagt
    39721 gtaactatag aaaaaatatg cttaagtaat gatggaatcg ttatagacac aggaatgaca
    39781 actttagaag atttcgattt agacccatta ggagatatag taggaagaaa ctgtgaaaca
    39841 acttttgaat ttgcagtttg tggagaaaga aactctgagt gctgtagaca aggaaaaggc
    39901 aaatcagtag cttataaaca aagaggatta actgtagcag ttcgtaattt agtactagag
    39961 ctaagaggta gatgtggatg tacagagttc gttgcattag ctttcccagc agttagagca
    40021 ggaggtggat gtaagagaag agttgattat gtagaattta cttttaacac actttcagca
    40081 ccaatatgct tgccagctga cggaagagct gttactttaa gacaagaata tcaaactaac
    40141 ttaactgtag attgtatagg aaaatctata ttaaaattag aatgcaacga atgttgtgaa
    40201 cctttctatg aattaattat accaaatgat atagatttag tactttgctt acaagaaaca
    40261 gttagcacat taataagtga acaaatagta gttttagcat caccaaatcc aatccaacca
    40321 agacttgttg atactttctc taaagtatgt gatttttcgc aatgtggacc taatcatgga
    40381 agtggaaagc caagttgcca cagatagata ggtagatata tagacagttt aaattataaa
    40441 taaaaatcat ctaaggaagg ctaccaaagt gtataattta cattggtagc cttcacgttt
    40501 ttataactag cgtatagatt ttagtgattt aaaaagcact atagtatgta atagacaagt
    40561 aaagattgat actatcgtca ttgtgtatat gtatgcataa atattaagtc ctggaattgg
    40621 aagaaataca tataaagcta ttagttgaat taacatgcca gtaatagtat ttatacttga
    40681 agctaattct tttcttattg aatgtaaaat gccagataga gtttgattta gggacataaa
    40741 tagtggaact aaaaacatag cttttagata ttctcctgca agtgtatttt taaatactaa
    40801 tatacataaa tcttttccaa agaaataaaa aaatattgat gatagtatgc ctacaaataa
    40861 agtaagaagt atggagtatt tgattttttt aattacactc ttacgctttc ttagagccat
    40921 ttcttgtgaa atagttggta ttaaatttac aataagcgct gaaccaacag taaaaggtaa
    40981 aaatataaaa ggcattacca tcccactgac aataccatac atacttaaag attgttgata
    41041 agtcattcct gataatgcta atcttgatgg tatcatcata gaacttatag attgcaaaag
    41101 tatgtttgac attctattac aagttatggg aatagacatt tttaatgttt ccatagaaga
    41161 attataaaaa tcttttatgc ttataatata cctattgtca aatgatgaat ctcgccataa
    41221 gcaaataagc ataaatataa tattactcaa ctcaccaatc gatataccta atagagctat
    41281 ataacaattc atagatttat tatttatgta cataacaagt aaaaaaacaa ataatatttt
    41341 accaatttgt tcaatgattt gtccaatagc agggattttt acattcttta tgccatagta
    41401 atatcctctt agtacgtttg aaagagttat tataactata gcaggacaaa cagctagtat
    41461 aaatagattt aatttagcat cttttaaaaa tttaagtgat aaaaaagaac tattgaaaga
    41521 tgcaactata gatataatta gtgctacgaa aaatgatata tataaagttg atataaataa
    41581 agcattagta ttgtgtctat cgtttaaagc ttttctcttt gcaactagac aactaagtgc
    41641 agtaggtata cctgttgttg taacagctaa gcaaatcatc aaaaagttaa aaatcatatg
    41701 atagatacct aaacctgttt cacctagtgc tcttgataag aatattttat ataaaaaacc
    41761 aaatattctt acaataaaat tggatataaa taaaataact gttgatagta ctaaataagg
    41821 tactttcaaa aaaatcacct ccttatcttt taaatatatt cacaaggagg tgtaaaaatt
    41881 tataatttta taaattctgg tttcaattta ttaaatttag atacatattt aaatcccata
    41941 ttttttagta atgaatatat gtagtcaaac tcacatgcaa cttgacttat atgatgtgaa
    42001 tctgaacctg tggttatgat ttcccctccc aagtctttgt acatttgaag tatatactta
    42061 gatggggtta ggtctggaag attatatcta taacaagagg tatttatctc gatacctttg
    42121 ccatcataaa tagcttgttt aagagtttct tcaattatat cagaaaatag tctatcatct
    42181 aaaatagtgt cataaggtgc atacctttta atcaaatcta aatgacctaa gacagagtaa
    42241 tctttataat tcttaacaat attatataaa taaagatagt aattttcata aacttcatgt
    42301 tgagttttat ctttaaaata attaccaagg tataagtcca ttgtatctat agcatggata
    42361 gaacatatta taaagtccaa tggatattga tgtgcttcct ttttgtatgt atccattaat
    42421 tgagtttgaa ctccaaattc aattcctttt tttattgata ttttatcttt atatttgctt
    42481 tgaagagtct ctaaactctt aaaataatct tcgtagacaa ttgaaaaaga atcatcagca
    42541 tatacatcat agtcaacatg gtctgtgaaa caaatttcct ccaaaccgag ttcaatagat
    42601 ttttttacca tttcttccat tgtagatttt ccgtctgtag agaaacttga gtgcatgtga
    42661 tagtcgtaaa acataaatat ttcccctttt tataaaaatt ttggtattat atatacatac
    42721 taaataatat caaatataca taaaagcatc aataaaatat aaatacaatg agcggtgata
    42781 aatttggaaa aatacagaat agatttaaat gagaaaaaat caactaaata tatacagata
    42841 tttaatcata taaaagaatt gataataaat aaagagttag tagaacatga aaaattgcct
    42901 ccaataagaa aactatcaaa ttttttacaa gtaaataaca ctactatagt taaggtatac
    42961 gagcttttag aaaatgaagg atatgtatat aaaattatag ggagtggtac ttttgtatca
    43021 agtttaaatt cttataaaag cggtatagat aacaggaaaa ataagattat tgtgcaaaga
    43081 gaagatttaa ttcattttga caatgggaat ccatctaatg atatgtttcc tatcgattca
    43141 tttaaaaatg ctgtaaatat ggcattatct aaagatggta gttcaatatt tgaatatgat
    43201 gaaggtcttg gctctgaaga attaagagaa aaaatggtag agtatctaag tgatggaaat
    43261 ataaaaacta ctaaggaaaa tattcaaata atatcaggtg ctcaacaagg tatagatata
    43321 gtttgtaaag gacttataag ttattcagat gttgttttta tggaagaacc ttcttacaat
    43381 ggagcaattg aagtgtttaa aagtagaggt gcaaaaataa tatctatacc tatgttagat
    43441 gatggaattg atataggtat tttaaaatta aagttggaaa aaataaaacc aagacttata
    43501 tacattatgc ctaattttca aaatccaact ggaatatctt actcaacata taaaaaaaag
    43561 aaattgatag aattggcaga agaacatgat ttttatataa tagaagatga ttttataagt
    43621 gattttgttt ttgaatcaaa ggacaataga acaattagaa gttatgatga taaaaataga
    43681 gtagtatata taaaaagctt ttcaaaaata cttatgccag gtcttaggat aggaattgta
    43741 gaaatgccaa atgagttgtt aaaaaaagtt ttatgggcta aatattcatc agatatatca
    43801 acaccaggtt taattcaaaa atcaatgtat tactatatga ataattttga ttggaagaat
    43861 tatttgagtc atatagagaa gatatatact gataagtata aattggcaaa aaacttgatt
    43921 aatgacaaat tgagtggtaa attaaaagtt agaaaatctt gtggaggaat aaactttttt
    43981 ttagaattac caagaggata tacatctcaa gcatttacaa attttatgtt aaacaaagga
    44041 gtgtcaatgc ttccagggac ttattttttt gacaatctag tggatgatag attctttagg
    44101 ataaatatag ctcgtccaag catggaagaa ctagaaaagg gaatatctat aataagtgat
    44161 aatatagatg agtttttttg tgaatataaa aacaagttag atataaaaag caataaatta
    44221 ttttattaaa taaatagagg ttttttagga ggaaaaaatg tttgacaaga agaaattaga
    44281 taggattaat gaattagcaa agaaaaataa agagggaatc ctttctgctg atgaaatcaa
    44341 agaaagagaa attttaagga aagaatattt agaaaacttt agagcccact ttagatcaag
    44401 actagatagt gtcaaagtag tttcaccaga agaatatgag caatacatga aaaacaataa
    44461 aaattagaac ttagtaggag gaaaatatga acttaaagta cgaatttaag aatgcttggg
    44521 attttgaaag aaaagaaaat actattgaac aaattatgca atattctagc aattatatgg
    44581 agtttttaag taagtcaaag actgaaagac tttcagtaaa agaaattata aaattggcta
    44641 aagaaaataa ctatatttca atagatgaag ctatggaaaa aggaagcata agttgtggtg
    44701 ataaaattta tgttataaat aaagagaagg cagtagcact ttttgtaata ggtaaaaatt
    44761 atatagaaaa aggtatgaag attataggaa gccatataga ttcgccaaga ttagatttaa
    44821 aaccaaatcc actgtatcaa gagagtaatc taggtttttt taagactcat tattatggtg
    44881 gaataaaaaa ataccaatgg acggctattc cacttgcttt acatggtata gttatattaa
    44941 atgatggaac aaaagtggac atatctattg gggaagaaga tagtgaccca gtattttgtg
    45001 taactgattt attgattcat ttggctggag accaaatgca aaagaaacta tcagaaggaa
    45061 ttagtggaga agctttaaat atattaattg ggaatatgcc tttagaagat gaagaaaaag
    45121 aacctataac agcaaatata ttaaagattt taaatgagaa atacaatata gttgaagaag
    45181 atttattaag tgctgaaatt gaagtagttc cagctggtaa ggctagagat ttagggcttg
    45241 ataggtctat ggtgttggga tatgggcatg atgatagggt atgttcatat gctgctgtaa
    45301 aagctatact agaaactgaa caaccagaat ttacttcagt tgcattatgt gtagataaag
    45361 aagaaattgg ttctaaaggg aatacaggta tgcactcaaa gttttttgaa aatactgtgg
    45421 ctgaactgat tgctttagaa ggagattatt gtgatattaa agtaagaaga gctttagcaa
    45481 atagtaaggt attatcagct gatgtttctg ctggatatga cccaaatttt ggagaagcat
    45541 atgaaaagag aaattcggca tatatgggaa atggagtagt tttaactaag tatacaggtt
    45601 caagaggaaa gagtggatgt aatgatgcta atgctgaatt tatgtcagaa gtaaagagaa
    45661 tttttaataa aggaaatgta gtatggcaaa ctgctgaact tggaaaagta gaccaaggag
    45721 gaggcggaac tatagcccat atattggcaa atcaaggtgc agaggttata gactgtggtg
    45781 ttggagtttt aaatatgcat gcaccacatg agattgtctc taaagtagac atatatgaaa
    45841 tgtataaggg atataaggca ttttttaata taaatttata agtaaaaata taaatgtaat
    45901 aagtgaataa aggctgttgt ataaacaatt taatctgttg tacaacagtc ttatttttat
    45961 gtaaaaatag caatttgtca cactagagaa aataaaagaa aatactgtca aattaaaaat
    46021 tattttaaaa aaaattgaca acgatttcat cttgttataa tatgtaataa agaacttgtt
    46081 atataatgtt ataaaaatgt tacataggga ggtgagcaaa atggatgaaa agagacaaaa
    46141 aaaagaaatg gatttagaag taaattcctt tgttccttta tatcaacaac tatatgacaa
    46201 cataaaaaaa caaatagcat ctggcatata taaaccagga gataaacttc catctgaagg
    46261 agacttatgt aaagaattta atataagtcg tataactgtg agaaatacga cttttttata
    46321 taaaatagca aaataacagt gtttgagcaa aaaaaaagaa tgttttttat cgttttgcca
    46381 cctgtttgcc accataactt ttttcggtgg caaattattg taaaatgccc tccaaaatat
    46441 ctattgtttc actttccatt ttacttgtaa catgtgaata tgtgtccata gtggttgata
    46501 attgactatg acctaatctt tgttgtatat attttacatt agctccattt tctaataata
    46561 atgtggcatg tgtatgtcgc aagcaatgaa aattaaagtt aatatttagt ttcttgttaa
    46621 tagttcttat agcagcgtca atattgttgt gatttacaaa tgagccatct ttttttctac
    46681 aaacccaatc atactcagtt tctttatacc attttccaat tttaattttt tgtttttttt
    46741 gattcaattt ttcctctttt aatatcctag acaaagtatc acctatttta atatctctta
    46801 ttgatgtttt tgtcttaggt gatgctaatt caaattctga aacttttctc tttattaaat
    46861 tttttctaac tttgattata ttattatcta aatcaacatt atcccaacaa agacctaata
    46921 tttctccttt tctcatgcct gtatgaaacc ctataagtag aggaatatag atatttgtat
    46981 ttttaggata tatttctagt attttattga actcatctag tgttatagtc ttgttatctg
    47041 actcattttt tacctttaaa acattttttg gtatactggc atattgaaca ggattttcct
    47101 taatgagttt gtaagggtaa acagcagatt ttaaagcagc atttaataca acataaatcg
    47161 cttttaaaac tccttttgtg tagtgttttt cttctccgtt ttgtgtgtat gtctcttttg
    47221 atttattatt taaaaattct tgaataatag ctggatttat agattttagc ttacatttac
    47281 caagtctagg ttctatatga ttttcaatta aatttctata actttcctga gtattgtatt
    47341 tgcaattaag aagtacatac tctttgtacc aaaagttaag atagtctgat aaactcatat
    47401 ttgtttcatc aaacactata ccagcatttt catattcatt tattgcatca cggagcgatt
    47461 tttcagcttc tttcttagtg tttccaccaa ctctttctac cttttttctc ttgccatcta
    47521 ctatacctgc atcaaagtag taataccact tctttccacg ttttcttacg ccgcctttca
    47581 taaaatctct cctctcaaaa gcttttttta tgtaattata taataacata tatcaattta
    47641 aataaaataa ataaaaaaga ctataagaaa caaagtttta tgtttataat taatagtctt
    47701 ttttatttag atacatattt aatgtttttt atcacgtgat tccagcactt tttctgccaa
    47761 ataaacaact attttttcat actcaatatc agtttctaaa gaaaagtcaa caatagcagg
    47821 tatcgtcata tctcctgcat atttattacc taaccaattt tcaattaatt ccaattcttc
    47881 tttgcttaat tcaaattttt tctttttatt aaaacttaag ttcaatttat tcacctcaaa
    47941 taatattata tattaaaaat agtatttgat agaaacattc gaattattta tgtaattttc
    48001 tttttttaat atattcatca gcaaaataat ttactaaata tttttcctcc aatcctaaag
    48061 ctatttctaa ttcaataaga gcagggatag ttaaatcttt attctcatta ttttccaacc
    48121 tagaaatttg acttctgtgg caaccaactc tttttgctaa ctctatttgt gttaatttct
    48181 tcttttttcg taactctttt aacatatatt taacctacct tttagataat tttatatatg
    48241 ttagtttgtg taatttcttg taataaatgt gcaatattcg cacacttttt gttggaaatt
    48301 tgtgctagaa tgtagttaag aaataacttt atctagataa agcaaaaata atagaagcta
    48361 taaaatataa taatatttta cagttatttt atagttttaa gctttgaaaa agaggtggtt
    48421 gtaactattt ttaagaacgt atgtttcgtg gaattaataa ataaaatatt ttatagggga
    48481 tga
//
LOCUS       DAEPIN010000023        47145 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.023, whole genome shotgun sequence.
ACCESSION   DAEPIN010000023 DAEPIN010000000
VERSION     DAEPIN010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 47145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47145
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.023"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            364..1236
                     /locus_tag="KR250_001534"
     CDS             364..1236
                     /locus_tag="KR250_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0067477.1"
                     /translation="MEMKIAVSNLSKRFGKKQALDNINLEIDSGMFGLLGRNGAGKTT
                     FMRILATLLSKTEGSIHMCGVPIENTKEIRNMVGYLPQDFSMYPNMTVYKAMDYLAVL
                     SDIPLRKRRKIIAKLLSKVNLTNYQNVKVKSLSGGMNRRLGIAQALIHNPRVLIVDEP
                     TAGLDPEERIRFRNLLCEIANDRIVILSTHIVGDIEATCENVAILNSGKVIYNGSTEA
                     LAKLAEGKVYSIEVDKKDIENIKSRFIVIGMLTHGGKAILRIISDDKPFETAVNCNPT
                     IEDGYMLIMGGDNI"
     gene            1233..2522
                     /locus_tag="KR250_001535"
     CDS             1233..2522
                     /locus_tag="KR250_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0067478.1"
                     /translation="MNLFIKECKNILKSMIYYIFIVVLVFFYISQLGSSVSNDVSKNI
                     PNDGNPLVAPQKVENIEEYYSVHNQYPYGQKESMKTEKVIPNATINLIREYQKNTYTT
                     YPTGVVKYIKLDQNKTKTIEKYIEEITGKSISEINSLWKTDESIKIDYSKVSLERFEE
                     IMQRVDDILGGGSAYAFDKLQRFGVVPVTYDEAMKNYNYTIQKDKVSNGYARIFCDYI
                     GIVLSIFPVFVAVFMSLKDKRSRMREIIYSKQVSSCKIVLSRYFALVFMMILPVLILG
                     MKDLLPLIFFAKDKGISIDNLAFIKYTLWWILPTLMIVTAVGMSITILTDTPVAIIVQ
                     LIWWLYSINSTGLKGDYSTMGLMIRHNSVLNGDIIIQNFNQIMINRIVISLGALVLVL
                     LAIFLYEQKRRGNLDVWAKFSKLFSSNKRKLQIKHFN"
     gene            2452..3096
                     /locus_tag="KR250_001536"
     CDS             2452..3096
                     /locus_tag="KR250_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0067479.1"
                     /translation="MFGQSFQNCLVLIKENFKLSTLTNLICSIILLCMIPLIQGVANL
                     DRISTAICLENYVAIIGIVMLVPVFSLEQSKEIDEIVSSKALSQVIVQLIRILMSIIG
                     IVVLIIGFVNVLIYLGCNFPVVEYIIGSFASAMFIGSLGMFFSNLFNNTIIGYMISIG
                     YLILNMMTKGKYVGKFFIMSMSQGSFDEKYWLILGSVVLIIASLLIKPVKRKIR"
     gene            complement(3363..3826)
                     /locus_tag="KR250_001537"
                     /pseudo
     CDS             complement(3363..3826)
                     /locus_tag="KR250_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861860.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(4826..5653)
                     /locus_tag="KR250_001538"
     CDS             complement(4826..5653)
                     /locus_tag="KR250_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HBH0067480.1"
                     /translation="MDLSKKVKILENASDGKSEFEILEFEKLEGASSPLMAMGLYFAR
                     EAGLKARQIKIKLNNSSIKTEAGALYYYKGNISSETKIGGVGGLFKKGISGALTNESM
                     MKPVYKGTGEVYLEPSFRHYLFMSLDNDSIIVDKGLFYCCSDTIEVKAFTQKNFSSAL
                     LGGEGFFQIELSGTGVIVLECVVPQSEIVEYELKNGEELKVDGNFAIARTSGVNFSVT
                     KSDKSLIGSALNGEGFLNTFTGSGTVWLAPTQVMYEKLSFGAMPTNDNMNNKVSKQK"
     gene            6336..6791
                     /gene="smpB"
                     /locus_tag="KR250_001539"
     CDS             6336..6791
                     /gene="smpB"
                     /locus_tag="KR250_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HBH0067481.1"
                     /translation="MAGKKILATNKRARHEYFIEETYECGIELKGTEVKSIRQGKVNL
                     SDGFASVDNSEVFLKQVHISPYEQGNIFNVDSLRVRKLLLHKHEIRKLIGATTIKGYS
                     LIPLSLYLKNGKVKVEIALAKGKKLYDKRQDMAKKDAQRRIEREMTGKY"
     gene            6949..7298
                     /gene="ssrA"
                     /locus_tag="KR250_001540"
     tmRNA           6949..7298
                     /gene="ssrA"
                     /locus_tag="KR250_001540"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            7413..9440
                     /locus_tag="KR250_001541"
     CDS             7413..9440
                     /locus_tag="KR250_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HBH0067482.1"
                     /translation="MSIDNNFNHVAFLWNIAESLRGTYKEEDYRKVMLPLIVIRRFDC
                     LLDDYDREIIKSVYEEYDFLPEEERDELVIVDLKENHDINLQFYNVSDFTWKKLLDDS
                     ENIKSNFEEYLNGFSNNVKEIIGKFKFKDEINQLDKKDKLYAVLSKMYEVDLHINSVS
                     NNKMGYIYEEMLRRFTENSAAGEQYTPREVIRLCMEILFMGKENFLTEEGKVISIADF
                     CCGTGGMLSIAEDYVEKINPSAIVNVYGQELLDESFAICQADMLMKGQNPDNIRLGNT
                     LTQDRFNGEKIRFLISNPPFGVTWKDEEKKVKEEADLGFDGRFGAGTPRVSDGSLLFL
                     QNMISKMYDDEEGSRIAIIFNGSPLFTGDAGSGESNIRKWIIENDLLEGIIALPTDMF
                     YNTGIATYIWVLTNKKEAKRKGKIQLVNASEYYQLMRKSLGNKRKEISLEQIEQIKEI
                     YEKFEESENSKIFDNEDFGYRKVTIERPLKLSFKVNEEAIENVKNTTQFINLAVSKKK
                     DIEVKEKEEAEGKIKQDKLIKLLESFDNTLEYMDRDKFIKDLKAKSKEFDIQLSAGLI
                     KAIVNTIGVRNEDAEVCKDSKGNIESDSSLKDTESIGLKDDVYEYFEKEVKPHVNDAY
                     IDESSINNIGYEIPFTRHFYKYEELRSFAEIMKEVESLESEIALKIRKVLG"
     gene            9440..10831
                     /locus_tag="KR250_001542"
     CDS             9440..10831
                     /locus_tag="KR250_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010233411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HBH0067483.1"
                     /translation="MGYRYRSDDELKDSGVEWLGKIPKDWEVRQLKRVLSDRKENNEP
                     IKTSNILSLTIEKGVIPYSEKGTGGNKAKEDLTQYKLVYPNDIVLNSMNVIVGAVGLS
                     KYFGCASPAYYMLYKINANDDIRFYNYIFKSKLFQDSLKGLGNGIMMKQSETSGKLNT
                     IRMRIPMEKLSSVKIPIAFNLDQEKIANFLDEKTSQFDSIISKKEKLIERLEEAKKSL
                     ISEVVTGKVKVVKTDDGYELIKRSSEEMKDSGVEWLGEIPKEWDIKRLRFLGKLQNGI
                     SKSSDSFGYGYPFVSYSNVYRNIELPHIVEGLVNSTEEERKIYSVEEGDVFFTRTSET
                     VEEIGFASTCMKTIDNSTFAGFLIRFRPNDKRLYKGFSKYYFRCDLNRKYFVKEMNLV
                     TRASLSQNLLNNLSVVLPKYEEQVEIYNALELKVSYINLSINKLKYQIEKLKEAKQSL
                     ISEAVTGKIEILD"
     gene            10893..12164
                     /locus_tag="KR250_001543"
     CDS             10893..12164
                     /locus_tag="KR250_001543"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0067484.1"
                     /translation="MDKNIFQININTDVLIRLRNQINEEYYISYRKEFYDKNNKKHRA
                     WDKICAIMDRLDDTVDYLNKLKLNTGKYRRSAFDFFEFMNQASVVMDCITHLAKIFEV
                     PYEEVETSTEIFNELGKEGKGTDKKYFEYLRSLCSVHPMETSRHKIYQENSFECSPYV
                     VWNDKSIWTNDASDLYAVVYTSKDNDSFKRIHIYMNQIFEYVKRRVNFIENIIEHIEK
                     YQQEIIEIFRNTPIKKEEDFESYIDYLRNLKKEIEIRYGNDSFHELNYLIELFDLPLS
                     NPDNEEKMNLYKNALKYAIKFEHNSIQNMTNEGYKNNGILYPQVNITTTLLEELISLN
                     SGSEERRKYRYAIEKTSYLSYDSCWSDREWAYDQIDDMMPFLERYVTFDKAQSDFEHY
                     VLVKLALYLECLENECLINKNVPNDKKFRIK"
     gene            12199..15180
                     /locus_tag="KR250_001544"
     CDS             12199..15180
                     /locus_tag="KR250_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HBH0067485.1"
                     /translation="MLPKEKHLEESIENHLVSIGYEQGHSQEFDLENCLFVDDLFRFL
                     ENTQKELLDEFKYNRGSNYKKAFIDLLNSNIKRRGLISVLRNGVEDIMMNGKFQLFYN
                     KPNLTTNKTAYENYKQNIFKITRQLKYSSKHNNTLDIVLSLNGFPVIVMELKNQFTNQ
                     NVYNAIKQFKKDRNPKETLFGFNQRVLVYFAVDTDEVYMTTELKGDKTFFLPFNKGNN
                     RGKGNPVVEGKEKTSYLWEEILTKDSLLDIIKRFYFIQEDDKGNQRAIFPRFHQLDAV
                     RKIVEDLKINKVGKNYLIQHSAGSGKTNTIAWTSHRLSSLHDEENNAIFDSIIVVTDR
                     KVLDKQLQNAIYQLEHKNGLVVKIDEDKNSSDLADAIVNRAKIIITTIQKFQYALPKI
                     EQLDKRRYAVIIDEAHSSTSGENMNALKETLAGKTLEEAKVIDAENEKNEKSSIDKMN
                     ELIEKRTNLGNISFFAFTATPKNKTMQVFGRIGEDGLPHEFHLYSMKQAIEEGFILDV
                     LKNFMPISVYYKVGKKIIDNPEFDKGEAKRAINKFVSLNEHNIRQKVETIVDDFVDNR
                     SMWLEGKAKGMIVTASRLHAVRYKLAIDQYIKEKGYDMKALVAFSGTVKDGDDEYTEV
                     GMNKEVAPDITDSNLPNIFDKNEFKLLIVAEKYQTGFDQPKLCAMYVDKKLDGVKTVQ
                     TLSRLNRTYPNKQTFILDFQNSVEDIQDAYKPYFEMTNIDKVTDPNVVNDLWYQLHEY
                     AVYTDEEVNDFTVLFYKEKRTSSQDAKMNNLIDKAVERYCYLEEDDEKKSDEFKKKCQ
                     KYITFYNFLIQIYPIKNLNLLRLQVYLVALLKKLPKKSKQRIDLDDMLSLDYYKLQKL
                     GLDDKKGTDISLYNGEGELVGISETATSRVSEADEEYLEDIIKKINDVFGVGLTEDDR
                     IMINTYEEMFKNDKDIMDIAKANSYEDLVSTFGKKYFKRGIVKTKNRNDRLVKEILTN
                     NNLQNYMIHYLAEKIYEEANR"
     gene            complement(15239..15409)
                     /locus_tag="KR250_001545"
     CDS             complement(15239..15409)
                     /locus_tag="KR250_001545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0067486.1"
                     /translation="MNNDKPFIEFEEEVDFILTECKKEGVSIDRDTIELILDLDLKFL
                     ELKGIATPLEDE"
     gene            15690..16025
                     /locus_tag="KR250_001546"
     CDS             15690..16025
                     /locus_tag="KR250_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0067487.1"
                     /translation="MIKLWKKEDLNLLLEYPKEVVENVDNVINILDENYGCNRKLTDD
                     GGYVCIIEDIKEVENLKSNILKGLVEEFSDVIYEDEVNTYNSTLYLLSSDYSVTVISK
                     NEETEYLLK"
     gene            16089..16547
                     /locus_tag="KR250_001547"
                     /pseudo
     CDS             16089..16547
                     /locus_tag="KR250_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427593.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
     gene            16687..16980
                     /locus_tag="KR250_001548"
     CDS             16687..16980
                     /locus_tag="KR250_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0067488.1"
                     /translation="MFKKENRYMTKEIAENLPIEIAILLWNLIDDLTTEKDYLQLFEI
                     NPIGLGVVEIVHKQEVPEYEARIYIQNDKIKDKLKIYVIDNVEYRTMMFSNEY"
     gene            17007..17603
                     /locus_tag="KR250_001549"
     CDS             17007..17603
                     /locus_tag="KR250_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0067489.1"
                     /translation="MINNKSEIINKLFFNELKELIDKYNNIDDKTVDVIEKIFTCIED
                     EEIKNYLIRDNCKLQELVNTYNKLEDLNIDEAIFFAWYNLTINTISIDKASEYYDDLT
                     TSNYIEVENHIIYTNERDLRKYAEDELETMLSMEHHIDRLLHKDMLIDMWLNNTSKEE
                     VIEEILMSDDIEDILNLSPEYAFTLANGIEYRYSEKEE"
     gene            17756..18025
                     /locus_tag="KR250_001550"
     CDS             17756..18025
                     /locus_tag="KR250_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003487728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mor transcription activator family protein"
                     /protein_id="HBH0067490.1"
                     /translation="MTNNNNNCDYKGIYADMVEVLGEEIVIKLHKYYRGQQITFPMKL
                     YSIEYVERYIVKNYRTKTIKEMCRELGYTEGWIKQLINKNRLNSK"
     gene            18051..18293
                     /locus_tag="KR250_001551"
     CDS             18051..18293
                     /locus_tag="KR250_001551"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0067491.1"
                     /translation="MILTVLAILMGTGIFFLINSMLDITYFGCGPIGFLWFGCFIVSY
                     AILTSLGGIVLGLLKWIVIGLIILFVIGLIANKIND"
     gene            18499..19632
                     /locus_tag="KR250_001552"
     CDS             18499..19632
                     /locus_tag="KR250_001552"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012938.1,HMM:NF016983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HBH0067492.1"
                     /translation="MQDFDFISETIKKFQSDLENMDKVNILVVGKTGVGKSTLINNIF
                     REELAETGIGRPVTQHLKKISKEGLPINLYDTKGLELEEDVQETIKKEILDEIENCNR
                     NSLERNDKSELMHVCWYCINTSVARCEETDIKWINELSEKIPVIVVLTQGFPRKKARE
                     LKSSIDDLNLNCKNVIPVLAEQLVEEEDEIVIKSYGLDKLVEVTYQVIPKEVKKAFVN
                     VQKVDIKKKVEESRKWVLGYVGGSGAIGFSPIPFSDAPLLATAQVAMISHITVIFGLQ
                     VDKALLTAIVSSIGGVTSATLLGKTLVSNILKFIPGAGTIAGGAISGSVAALTTTALG
                     MAYISVLEYILNENMKGNEVNNTEIAEKMKDAYKDQLGMLSKK"
     gene            20396..20641
                     /locus_tag="KR250_001553"
     CDS             20396..20641
                     /locus_tag="KR250_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0067493.1"
                     /translation="MRSVTVEDGDERFAIEKIYIKSLQRYEVRICLYRDCRDRINKLI
                     PRPVDLTMDKFVALILVGLKAGVLDDDFKQELIKGLA"
     gene            20989..22350
                     /locus_tag="KR250_001554"
     CDS             20989..22350
                     /locus_tag="KR250_001554"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0067494.1"
                     /translation="MKGNINKKDIDLSKITFDKFLEDEGLQNRERDYLYVDDYDNTLD
                     TQEFKLHIIEENTDNTYIDKDKYWYLDLDDSLFEYEEDEDTIAKTLYSLIDIRIKFLK
                     HVEYRKYINLGVIDEEYFDEIKSFLKFYIVAKTKKNELGNSNLKQMAIDIFDKERFED
                     DISDDDMENISEWIGEDQNFKMFYDKVCRDLSNSTQDKEILKLAGIRNNKVKHYKGRK
                     ISRKCKLELKSLTKMWEYETKLTHDANKAHKKIKSNMLEKMTDKNYKNIYEYLNNEII
                     NSNEEYKNIYFYSLELKLANELRKCTLKNLNKIKDIENQVKFISYTIAYIHIVPVLTI
                     REKLSDKLFNILSTNEGKDIEKFTKEVSLIMRIINIECLELLNIVSKNNYYKNFSEKE
                     LEEFKSIYNINNGRKSFENKYKIKVDYNIDDVRKYGEVIKAINIESKKKIVDRIKKKR
                     KSN"
     gene            22540..22857
                     /locus_tag="KR250_001555"
     CDS             22540..22857
                     /locus_tag="KR250_001555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0067495.1"
                     /translation="MTNFKSVKVELILLIECEEGNRNELEGMIDEGVLNEKEYSLTIL
                     GSTEYEDNARAIYILMNTGGSYEKNLQRLSRLHLKIENLLKDTNVKYRGISLVPNIVK
                     WDK"
     gene            22869..24632
                     /locus_tag="KR250_001556"
     CDS             22869..24632
                     /locus_tag="KR250_001556"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0067496.1"
                     /translation="MSKAINREELIKLYEEIGLNVLDNNTVMNSSNKKICNFLVNIIE
                     QRVIDGKMYLCINAMGINGELLSEVILDSNTINRTTWVIENWGFKYSIDKKYINDFIE
                     LIQHLCMNIKTKYIYDHTGWIYQDNKWIYLHGGGVIGGSDVQVELDESIDNYILPDKI
                     SDINKACEMSLKLVDLIEDDKGIIFSSLVYLSPLIEIISRKIKPPENIVWTYGKTGSR
                     KTAVSKVVLSHFGDFNKKIPATFNDTITAIELKANKLKDSLYLCDDFAPKQDFRDKKI
                     QDSKAESIIRMYGDRVGKGRSSNKLDIKDAKRPRGMILITGEDVICGESSNARLLSIE
                     LNRSSVDLNILTELQNNSHLLGEAMRGYIEWLLSEMGNIDELSNILLYNFKSYRDEIR
                     EQYKDDIHGRTIESSAWILVGFSCMLNYIRDKGVIDKYEDDEYMDRAIKVVERLIEEQ
                     IKLISRNSPIDIFLNTLKESINSKSIRIATLNDQNQVIEDKLEYICGYKDKEYYYFYT
                     DKVYSFIEFENKKRGIHLGISQRQLVKQLRDSGIVKVDSNNDSPKKTIHTKDASDSTG
                     YTTIRPRMLQIERSIIENFQI"
     gene            24695..24850
                     /locus_tag="KR250_001557"
     CDS             24695..24850
                     /locus_tag="KR250_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0067497.1"
                     /translation="MTETYKISIGRKDLYSKDYKVVERTDYEKRHTVNISRSLVDILS
                     ILVKEDR"
     gene            24937..26502
                     /locus_tag="KR250_001558"
     CDS             24937..26502
                     /locus_tag="KR250_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBH0067498.1"
                     /translation="MTGKKAVVYARFSSDNQRDESIDAQLRAINEYADKNNIKIVNQF
                     IDRAKSATSDKRPEFQNMIKFCEADTTGISMVIVHKLDRFSRDKYDSAMYKQKLKVKG
                     IRVVSVLENLDNSPESLILESVIEGMAQYYSANLAREVAKGQKENALKALHNGGDAPL
                     GYDVAHDKTYTVNEEEAQAVKIIFDRYIKGYSYSKIIDELNILGYKTKRGNKFGKNSL
                     HGILNNEKYTGVYVFNKTQRKGVDGKRNGHKQKSEEEIIKIEGGMPQIIDKGVFKQAQ
                     DMIQKRKKTPGAHKATTTYLLTGLIKCGECGHAFQGNKRKDSYGNEYVSYRCGCRKQK
                     RECNNREIKRDYLEEFILQELEKNILNDEAIPILSKALNEKLNDKNESNAELLKNLEQ
                     KLDKVNKEISNILNAIMNGIVNSMLKDKLDELEQVKFNLDLKMNELKIENKVVDGVGV
                     TEDQIRGMFSRFKEFVLERNIPECKKFIGDYIKEVIVYKDHIEVIFNVVFCFVDNDIT
                     HDLHIITERGKIT"
     gene            26648..27940
                     /locus_tag="KR250_001559"
     CDS             26648..27940
                     /locus_tag="KR250_001559"
                     /inference="COORDINATES: protein motif:HMM:NF014177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HBH0067499.1"
                     /translation="MADIFTVLNYQGSKKRLLDFIYENTQEYIDSDKAFLDICSGTSA
                     VAYNMKRHFTVYTNDSENYAYQISNSIVKNNERYNFSQSDEMIIREKYNINLQRLNNI
                     FNDMVNQEDMLLEQEDADGLIELYSNCPCIWKNNVDIEIEGNTIQSIYDLRKCKRDIP
                     NVLFTLYYSSSYFGIKQSMEIDSIRYAIEFIESEITKSILFTSLFFAMKECVFAKDGH
                     MAQPLNMETGKKRMLRLKARSIYDIFIDKLKEFNTEEFVLSQKENKCFNKDFRTLLET
                     EESIKDEVGFIYIDPPYTDMQYSRYYHILNTVVLYDYPEISLSRGTISKGIYREGRYQ
                     SPLSQRKHALNEIDFLFSFCKENKINVALSYAYPIDTEKQATNRYTASIDDLINVAKK
                     HFGDNVKVVHEEYSHANNRNSDKKNVLEYLIMGIHNKE"
     gene            27943..29562
                     /locus_tag="KR250_001560"
     CDS             27943..29562
                     /locus_tag="KR250_001560"
                     /inference="COORDINATES: protein motif:HMM:NF013704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HBH0067500.1"
                     /translation="MDKINKLKERIANIEATNKNDIYSSNLYWSQKPFNICDEIIEHL
                     SEDGDIIFDPFMGSGVTVIEALKKEYKRNAIGCDINDAPIFIVKTLLEKYNVPKIEKD
                     IKELNNKLSKLSTYYSTKCRECGEVVTISKTIFDKENRNAKCELKSIHYKCKECKNDF
                     KGEPNETEISKFFIDEELKNIDDFQFIANSRLAVGEGDTVRTIFSNRNLIVLDKILGH
                     IDEYKNSESYEPMKYILLSILHLSKITDTHSNSQWPLWIPKKDCVEKNVVELFIKNSK
                     KTIKALKLASKYLCENRENVELIKNLDRGKFKLIKDGVQNIEDDVIPNEFVDLIITDP
                     PYMGQVLYSEYMQLYKPFMDLEFELENEIVISSAPARDKGKVLYFDMMNSAFENISRV
                     LKVGKYMCMYFHDSNLDVWDNIIDIMTNNNLKYMGQIHVSKNKNTLKNILSPKKSLNG
                     DCIIFFKKDLNINYDINEVDNIEDKINNIAQAIIDIKGYASTPELYDNGALEYIISNG
                     KLKEISKKYKDLTSIFEQRFNWDKERGVWTV"
     gene            complement(29687..30838)
                     /locus_tag="KR250_001561"
     CDS             complement(29687..30838)
                     /locus_tag="KR250_001561"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0067501.1"
                     /translation="MKKLTIRLKDDFHKDLLDLVAFKNDSINSYVINLIKNDLSKNTN
                     LLRSYDNMIRDNITNVLITQKINELKAIQDGIFKEYKVNDILSNSKIYEVMTANSLNH
                     ILIPGHSGSRDAKDEFGNEFEYKHFKKSSSNHSWTFNDFSDTSIAKLKKAKAVIFAHI
                     NDVDFPYPGKLDWYYSIPGDIMCEYLLKYTQSIQNSRKMINVSPNQLESRLNASKIIP
                     SAQNGVYDKYLSAIYKASSELEELTGVKNLLTSNKFWELIVALELNHQVNPEQGGREG
                     AHDAYDSEGNPYEYKVSKNHAWNFQDISDNVLNKYYNDKAIILAVVDKENIEVEHIYS
                     TEPADVVPLLRKKLQEKINRCLEKGSPIRRLQVSLSKSDLTRVNAKKLY"
     gene            31360..31575
                     /locus_tag="KR250_001562"
     CDS             31360..31575
                     /locus_tag="KR250_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3791 domain-containing protein"
                     /protein_id="HBH0067502.1"
                     /translation="MDERKLEFSIFCIEALAEKLNIDSEGVYKLLKHDTDILDNYIIP
                     CYEALHSQSKGYIVEDLMQVLKEKGVI"
     gene            31575..32054
                     /locus_tag="KR250_001563"
     CDS             31575..32054
                     /locus_tag="KR250_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3990 domain-containing protein"
                     /protein_id="HBH0067503.1"
                     /translation="MIVYHGSYCVVKTPSITFSRDSLDFGKGFYVTSIKTQAINWTER
                     FKKRGKKAYLNSYSLDIDKLKENCKVKIFDSYNLEWLDFIIECRNMSDIYMKYDIIIG
                     GIADDKIYNTIELYQDNLIEKDEALKRLKYYKPNEQICITNQNVIDKYLKFRYEEEV"
     gene            32060..32290
                     /locus_tag="KR250_001564"
     CDS             32060..32290
                     /locus_tag="KR250_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0067504.1"
                     /translation="MEANKTLLQILYKKIILEFSKQTGKSLEESMDYLYTSETYKLIS
                     EGVSDMHCKGHIYLAQELMLENGLMYHKGYPR"
     gene            complement(32406..33572)
                     /locus_tag="KR250_001565"
     CDS             complement(32406..33572)
                     /locus_tag="KR250_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBH0067505.1"
                     /translation="MATKRANGEGSIGKYKDGWRSRIMIGYNEDGKPLRKEFYGKTQK
                     EVKDKLDAYKKQYYLSSDISDDKITLEHWFYNWLFEYRIKDLKPKSFERYESIYRNYI
                     KDTDLGNVKLKYLRVSHIQKYYNRLLDSNKSIPTIRQINTKLKTCLSEAEKQGLIQRN
                     YCTMVNIPVEKKENKLEILTLEQQKSFIKAIDGHKLEVLFLLALGTGLRLGELLGLKW
                     FDIDFKKSNLTVKRTLQRTYFIDKTGNRELKVLEQGPKTSNSYRTVPIPKDVLNKLKE
                     HKVNQNTDILKAGELYINNNYVFCNELGIPIDDKRPLRNLKSILNSLNIEPIKFHGLR
                     KTYATRLFENDVPPKTVQVLMGHYDISITLNIYTQVMEDKKVEAVEKLDKIFSL"
     gene            complement(33562..34314)
                     /locus_tag="KR250_001566"
     CDS             complement(33562..34314)
                     /locus_tag="KR250_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HBH0067506.1"
                     /translation="MNKSYIPKYTHKFPTNIPHGDKKYTLTYIRRMISEFVYDMGNLE
                     NNPFTFPEVQTLLDGITVGGHKLSDQNQILNIKSSWDYVIKLSNKENLSLNKDTICSI
                     HKIVAKDEALIVGDFRNGNIGIAGTTQYECIEATLLNDLFVNDINIINKITNPLEKAI
                     IINLWLSYCQFFYDGNKRTARLTSNLILINNDIGVLSIPAKHKQEYNTLMLNFYETLE
                     ADEVIKFLLEKCITFFDGYNYKSYKELFKNGN"
     gene            complement(34406..34897)
                     /locus_tag="KR250_001567"
     CDS             complement(34406..34897)
                     /locus_tag="KR250_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH0067507.1"
                     /translation="MMNNTIGEKIKQYRKSINMSRDELANKIGCSVHAIAKYEQNQRM
                     PSLDMIRKIATALSIAINKLIVEENDLDTLTNNNINLKVFDKELDLVIHILENYGYDI
                     KTDYKKKFEITDNADTIKPINLNFSDFKDFSKNLNWVINGLVENFIQENSNGLIEDED
                     EEY"
     gene            complement(34894..35092)
                     /locus_tag="KR250_001568"
                     /pseudo
     CDS             complement(34894..35092)
                     /locus_tag="KR250_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729178.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(35149..35910)
                     /locus_tag="KR250_001569"
     CDS             complement(35149..35910)
                     /locus_tag="KR250_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0067508.1"
                     /translation="MQIGKNLKKIRKQKELTQIQLAEISGISRNALINYENDKRIPSI
                     DTLSKLAKALKIEKTIFYFDIENEVMTNEDVLYLLQSLFPEEYYSFDEFLNAISNLME
                     DDIRITSSLLLGAWSDKDQYYKLGEKLELNENQVYNWILSDCISNTELDFSLHPDDIK
                     EIIKNDVLTKQSIDELLDIGLSEESIETIYDYFSDSTDNGSNIRLKKLISQKENITIK
                     NNDYFKLLQENNELKEKNEKLENTLKEIKKLLTKI"
     gene            36057..36257
                     /locus_tag="KR250_001570"
     CDS             36057..36257
                     /locus_tag="KR250_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0067509.1"
                     /translation="MGINLKIFRIKKGLKQQELADRVGISRYYLSNLETGKANNPSND
                     LMIKLSRALDTTVEELFFSKED"
     gene            36261..36575
                     /locus_tag="KR250_001571"
     CDS             36261..36575
                     /locus_tag="KR250_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0067510.1"
                     /translation="MKENNNLEVLKTLQATLNIIVKNEEEAQEKKQGYKIIENAKRNK
                     AELITCKEASKLYPIGESKFRQLCHSKNKGFPCIWINNRVYIIKNKLDDWFIENANGI
                     KL"
     gene            36597..37340
                     /locus_tag="KR250_001572"
     CDS             36597..37340
                     /locus_tag="KR250_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0067511.1"
                     /translation="MSNKGWIKLYRELLDKSIWKDIKPERKVILITLLLMASHKENHV
                     FIDNKKIKIKFGQFISSYSKIAERCGKGVSYQMVRSCLLVFKNAGFLTYETTNKYIVI
                     TIENWELYQENIENQQPNQQTINNQSTTIKNIKNENNIIYSEIISKFNSTCIELSKVK
                     KLTDTRKRRINSRIKEYDKETVLEVINIVSKTSFLNGNNNTGWKANFDWIMNSSNFTK
                     ILEGNYNSKEIVINKETGIYNPDDSQLKW"
     gene            complement(38046..39959)
                     /locus_tag="KR250_001573"
     CDS             complement(38046..39959)
                     /locus_tag="KR250_001573"
                     /inference="COORDINATES: protein motif:HMM:NF020926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1998 domain-containing protein"
                     /protein_id="HBH0067512.1"
                     /translation="MSIKLDDKTHNLRINQATSVFGPGAMLDFIDQTLMTAHPNYWTN
                     TIKIQDDRLQATLRVNELRTPPTIDDRSSVPFVRFPRWYFCPKCRKFMPIEDWEKLYK
                     NAHKDKPMKIPICLTCSTKKSTKQLVPTGIITICEHGHIDDFPWVKWVHFRNKSGRKE
                     ICENPQILMKTGKSNLGLEGIELECSCGAKANMKGAFMRLKSSKSNDYGVPSEYKEMF
                     KCSGNSPWYNKKVNKCTKYSFASQRGASNVYYPKIESSIIIPPYSDTLNSKVLSSKGY
                     DTFKKFLDRAIKKNKLERFMDEDFEDFASDIAKETGFDTDKVSIVLKDLLPFEDSSPS
                     TTTRNQYREEEYEALIGNIDAKSKKTNDFTIEEQNLSDYDIPNFSKIVLVKRLREVRA
                     LTGFSRVNPPDNNIFGEEDIDDSCNDTKVISLRSRDCKKSFYPASEVRGEGIFIEINN
                     DMVKSWISSNPKIQDNASILNSRYNKDINVNDKAYRKITPKFLLLHTLAHLIIRELSF
                     VCGYATSSLRERIYCNNSENEKVMSGILVYTASGDSEGTLGGLVRQGMHDSLPSILNS
                     ALNRALWCSNDPICIDSTGQGRNSLNLGACHSCTLLPETSCEEYNLLLDRSMLIGDMD
                     NRDIGFFKNLILI"
     gene            complement(39961..43488)
                     /locus_tag="KR250_001574"
     CDS             complement(39961..43488)
                     /locus_tag="KR250_001574"
                     /inference="COORDINATES: protein motif:HMM:NF012493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HBH0067513.1"
                     /translation="MNCSELRDKTLDLVRKELLGPGSEDLCENIKYEIITDSPIERYS
                     TGILYPLNNNIDVSNTSEYDGEDNVFTSDDTQNELDRAISMSNETLPSSMGMTFFLKK
                     DAEVINFRVNYATYRNALYTDCHVKYTGDDFYSSNTINEYVELRGSFLYLKQKLTSEK
                     VYNLLKKHDLLDNAEAKFVLYKLANQCPDDENKTGGYVRVPHENLIVSIDMDKNYIKK
                     PISKDLDIVLSKRIYSNDFVSITCMLVNSQSGPKKYNNTIFQPELMVLAEDNNDLEFI
                     DIFTAGSVNTFKKLDSEEISLDLLYRNKKTFATGHGVAIDQDIQNDALIKLCTEFIPH
                     FEVPKLDFNVDELSDVSRELLSMKNLSDISDLSKDKKIYFLYKFVDAYDIWIDDLEED
                     SKDLSDVFKDVAYEHIKNCRDTKSRMLEGINLIKSNDEVYRAFSLMNRAILMQRCHSI
                     HITDRLPGSNNLDYIDYSNDNSAWRPFQLAYILTCLSSIENPDSKHRDLVDLLWIPTG
                     GGKTEAYLGLSAFTIFLRRIRNKENGNGTAIIMRYTLRLLAAQQFIRASILICACEII
                     RKENKSELGKKEISIGLWIGSDQTPNFNDEAEEMLNNLTGNNIKFRSSINSEYELDNL
                     KSKYNKFQLLKCPWCGTKLVKEWDKTSKKAVGDWGYRIKSRPKKFYLCCPEEDCSFED
                     MLPIQVVDEEIYNNPPTLLFGTVDKFAMITWTTEVASLFALDDDNSNLSPELIIQDEL
                     HLISGPLGTMVGLYETALDAMCSYKGIKPKIIASTATIRKAQNQVKNLYNRNVAQFPP
                     PGLTSSNSFFVKEMPLSKKRGRLYVGVIPSGKTQVTVEVRLMSALLQRLKMMENVELN
                     LLDKYWTLVSYFNSIRELGKCSTLLNDDIKDNMERLCKRVSAHPYQSLRNLNEQRELT
                     SRLTSTKINTTLDELSNTLTSNNLTVDTLLASNMISVGVDVDRLNLMVVVGQPKLTSE
                     YIQASSRVGRSNEGLIFTLYNPTKTRDRSHYEQFFSYHQSFYKFVEPTSITPFSEPAR
                     ERGMHAVLISMIRHILGLQANDLASRFSKNMDGVEDLKNFIIDRSIDIFRENNQNADD
                     NIVNEFKSEISSELDIIMDLWHDKAQISDKDNLLHYYKRPSKFDNKIYNNLLIPYDKD
                     VITNEKQTLNSMRNVDKQCTVEIIELEEN"
     gene            complement(43506..46124)
                     /locus_tag="KR250_001575"
     CDS             complement(43506..46124)
                     /locus_tag="KR250_001575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0067514.1"
                     /translation="MLTTNIHYILDNDICEIYFNKQRLTNSKYYQDNINVLNYIINEK
                     NNNLIINYNDKYIYAIYLLNIAINSYLNNTLNPNNHILTKLDKGMPLLYKGQIVIFKN
                     INNTHITLGHKNKTDTTLIPIDSAYMLITYNGHLEVNSMGKTPASKTFQTKNVLSSLG
                     FSDLKNMTGVINDSTLIILPCKDDISDLVSNIKIKDINNTYKFTELFPCSYISSTGAE
                     TDYPGNHAKQIPLLKFTTNISSAFEIIKSDKSIKNVFIIGDYPIYKHINDFERILNRK
                     RIESVNIVTSNSNISNILDLPNIDNLNIYSWSKDVLLTYSQDFENTSTFQKLWLDKLI
                     NKNICTTSVDSNISDLIHQARKSLYYISKYDFDISIKNSLMMCSYSLLKILENTPFNL
                     DFLEETIKLLNLNIATPSAKLENIKYLISSTSPDLELSALFDDVIVKFERIINELSDN
                     NTKFNTLNKLVSSYKYKTFTPNTPVILLQKQYEAIILKNLLKSRNINLQVISAEKFKD
                     SKTIKNLIMFGFYDLKYLDILNSDLIINITLYLYNSELSKYNYYMNQINSARNKIEKN
                     NKIYNLLDIKPFKPTYETNKLEAYSTDSPISDDDSMDEYISNYKFDINLNEIILSLSI
                     NQGIYNSKKEHVTSFMISDNNEYIFCTKKAIAYSIDWDKKSVRKKKLSEAKVNDWFLF
                     VDDYLNEEGELVINVIQKLLELNLLPSHYLDDYKLTNSWKSELKNYISYSKYNYNDVS
                     NSLAVLGEKINPITIKNWVTNKRLIGPIYLKTFEKIFKVIDSKLDHTEVYDATKSIRS
                     LHTKIKKSIDTFVSDNFFSSKSDIIKDEVSAVVLSMLGNTNDYVTPVQIREIHSCDKY
                     LSPHLVNKVLDLYSVY"
     gene            46436..>47145
                     /locus_tag="KR250_001576"
     CDS             46436..>47145
                     /locus_tag="KR250_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HBH0067515.1"
                     /translation="MYNVIDLFAGAGGLSLGFEMTKKFNMVAFVEKNDNAAKTYLENH
                     PSVKRYCDIKRLDFQDILNSVDKIDVVIGGPPCQGFSNANRQKRKIINGNNELVKLYV
                     DAIDKLKPNVFVMENVKTISSNKHSFYLTKKDKNHIINNLKLNIYNKDSVLYDRTEYI
                     NELFNIISIEDIESYIISNIDLKNLKIIIKKKKDICDYLNKISNYKKVKSTIEKLETR
                     GKMPKWYLELINKVKNIL"
BASE COUNT        18687 a         5222 c         7276 g        15960 t
ORIGIN      
        1 atatataaat tagttttaaa tttttgcaat atatagttgc agatattttt atttatctta
       61 aaaggctaaa taccctatcc tattatatcc agtaattctt ttggatactt atcacatttt
      121 tcattatata taaaaataga ctcacaaata aaataaatat atattaaatt ataacttcat
      181 aaacttaaaa aatttcagaa acaaagatac tttaatttaa ttaaaaacag aaaaattaaa
      241 atctttagaa aaaatttaga aatttataaa taatatctta agttttagaa tttattatga
      301 aaatacagta aaaatttata aaaataaaat tctataaaat tgtttctggg ggtgtattta
      361 tatatggaaa tgaaaatagc agtatccaat cttagtaaaa gatttgggaa aaaacaagct
      421 cttgataata taaatcttga aatcgacagt ggtatgtttg gtcttttagg aagaaatgga
      481 gcaggaaaaa ccacatttat gagaatttta gccactttat tatcaaaaac agaaggttca
      541 atacatatgt gtggagttcc tattgagaat actaaggaaa ttagaaatat ggttggttat
      601 ttgccacaag acttttcaat gtatcctaac atgacagtat ataaagcaat ggattattta
      661 gcagttttat cggatatacc actaagaaaa agaagaaaaa ttatagcaaa gttattaagc
      721 aaagtaaacc ttactaatta tcaaaatgtg aaagtaaaat ctttatctgg aggtatgaat
      781 agaaggcttg ggatagcaca agcattaatt cataatccca gagttttaat agtagatgag
      841 ccgacagcag gtcttgaccc agaagaacgt ataagattta gaaatttact atgcgaaatt
      901 gcaaatgata gaatcgttat tttatctaca catattgttg gagatataga agctacatgt
      961 gaaaacgtag ctatattgaa tagtggaaaa gtcatataca atggctcaac agaagcatta
     1021 gcaaaactag ctgaagggaa agtatatagt attgaagtag ataaaaaaga tattgaaaat
     1081 ataaaaagta gatttatagt tataggaatg ttgactcatg gtggaaaggc tatacttagg
     1141 attatatcag atgataaacc ttttgagaca gcagtaaatt gtaatccaac tattgaagat
     1201 ggctatatgc ttataatggg aggagataat atatgaatct atttataaaa gaatgtaaaa
     1261 atatattaaa gagtatgata tattatattt ttattgtagt tttagttttt ttctatattt
     1321 ctcaacttgg aagttcagtt tcaaatgatg taagtaaaaa tattcctaat gatggaaatc
     1381 cattagttgc tccacaaaaa gtagagaata ttgaggaata ttactctgta cacaatcaat
     1441 atccatatgg tcaaaaagaa tctatgaaaa cagaaaaagt cattccaaat gcaactataa
     1501 acctaattag agaatatcaa aaaaacacat atacaacgta tccaacaggg gtagttaagt
     1561 atataaaatt agaccaaaat aaaacgaaga ccatagaaaa atatatagaa gaaataactg
     1621 gtaaaagtat tagcgaaata aattcacttt ggaaaactga tgaaagtata aaaatagatt
     1681 attctaaagt atctcttgaa agatttgaag aaataatgca aagagtagac gacattttag
     1741 gaggagggtc tgcatatgct tttgacaagt tacaaagatt tggagtagtt ccagttacct
     1801 atgatgaagc tatgaagaat tacaactata caatacaaaa agataaagtt agtaatgggt
     1861 atgcaagaat tttttgcgat tatataggta tagttttaag tatatttcca gtatttgtgg
     1921 cagtttttat gtcacttaaa gataagcgtt caagaatgag agagattatt tattcaaaac
     1981 aagtatcttc atgcaaaata gttttatcaa gatattttgc acttgtcttt atgatgatat
     2041 tgcctgtatt aattttaggt atgaaagatt tacttccttt gattttcttt gctaaagata
     2101 aaggtatatc tattgataac ttagcattta taaaatatac attatggtgg atactaccaa
     2161 ctcttatgat agttacagct gtaggaatga gtattactat attaacagat acaccagtag
     2221 caattattgt gcaattaatt tggtggcttt acagtataaa ttctacagga ctcaaaggtg
     2281 attattcaac aatggggcta atgataagac ataatagtgt attaaatggg gatattatta
     2341 tacaaaactt caatcaaatt atgattaata gaattgtgat aagtttggga gcattggtgc
     2401 ttgttttgtt agctatattc ttatatgagc aaaaaagaag ggggaatctt gatgtttggg
     2461 caaagttttc aaaactgttt agttctaata aaagaaaact tcaaattaag cactttaact
     2521 aatttaatat gttcaattat attgctatgt atgattcctc taatacaagg agtagctaat
     2581 ttagacagaa tttcaacagc tatatgttta gaaaattatg ttgcaataat agggatagta
     2641 atgttggtac ctgtattttc gctagagcaa tcaaaagaaa ttgatgagat agtatcttca
     2701 aaagcattat ctcaagttat tgtacaatta attaggatac tgatgtcaat tataggtata
     2761 gtggtattaa tcataggttt tgtaaatgtt ttaatatatc ttggatgtaa ttttccagta
     2821 gttgagtata ttattggaag tttcgcatca gctatgttta taggaagttt aggaatgttt
     2881 ttctcaaatt tatttaataa tacaatcata ggttatatga tatctatagg atatttaatt
     2941 ttaaatatga tgactaaagg caagtatgtt ggaaaatttt ttataatgtc tatgagtcaa
     3001 ggtagttttg atgagaaata ttggctgata cttggaagtg ttgtgttgat aattgcatct
     3061 ctcttaataa aaccagtaaa aaggaagatt agataataaa atgagagctt agataaaata
     3121 aaggtaatgc aaataaaaag tacatttatg aaatataaat ctatttttat atacacaaac
     3181 ctacttacaa tctctttatg tatgtagata gatttatata tccgtagtat aaaatatata
     3241 tgtataccga attttatcaa aagctctcaa atttatattt caaagacact tattgctatg
     3301 acctacacat atgtattttt tcattttaat tgttgtatta acaataggac tattgttata
     3361 aactattgat acctcataac aaaaaattct ccatctccta aatgcgtttt ttgtacctta
     3421 gtctcaacta ttttaaatcc ttgagactca taacatttta cagctctcat gttccaggtt
     3481 cttacattta atacaattgg tttagtacta tatttatttc tacattcttg taatcctagg
     3541 ctaattattt ctttaccaat tcccattcca caataattcg gtttcaacca ataccaaaga
     3601 atacagagct tccttcatct aataagttta taaaacctat caattcttta ttgtcattta
     3661 taaaacttat aaaatttttt cttttatctt cttttgctag tgaaaaattc ttctttttaa
     3721 tttcctccca tggaggtaga ttgtatattg aatatttacc ttcatatttc caattagtaa
     3781 cttcaatagc atgttcttca gtaaatttaa ctttatttaa attcatatgt cctcctatta
     3841 aaatatttaa ggtaattaat ggtattttaa agttttagaa aataagtaaa actatttata
     3901 tttatccata aaatctttaa aaattttact aacaattctt gtaccctgta ctttttattg
     3961 gattcacaga agactataaa ctaattttga atcaccttct aattcaaaaa catcacgata
     4021 tatataattt cttagcttga ttaaattctg ttaaatccgt ttcttatagt tgtataatca
     4081 acatcaatat ctttatgtgt attagttaat ttattggttt taggaactta ttattaatat
     4141 attgtatttg ctccaataat ccaagtagta ggtaaattat aggcaaatta tcaaatgtat
     4201 atctatccat gcttaaactt tagagataca ataactacta aattagtgtt ctagtagtta
     4261 caaaaaagaa tctttaaaaa tattaattta acttatattt tttaataaat tttctaaaat
     4321 ttaataagtt aatttaatta tagctctaaa tgatattttc tacagatata attttaaaaa
     4381 ggtgtgtata agaacaaatt cacttaaatt tttaaaatca aataaagttg aatctcttga
     4441 ctaccttgat tgttttatga aattaatata gatgtaaact ttactattaa aaaaatacaa
     4501 gattttagat aagttaatgc taattataaa taataagata agaaatataa tatgacataa
     4561 gggctaggaa taagataaat ataatcaaag aataagatat gagaataact accaagagaa
     4621 atcattacct taatatgttt ataaatgtta gactatgttc ctaaattgaa ttttatatat
     4681 caagtataat aactttacag aatgtggaaa tttttattta aagaatacac ataaatattt
     4741 ccacactctg attttataat aatctaataa aatttatagt gactttataa aatcaataat
     4801 gatttaataa atatttataa aacaactatt tttgcttaga aactttatta ttcatattat
     4861 cattagtagg cattgctcca aaacttagtt tttcatacat tacttgagtt ggagctagcc
     4921 aaactgtacc actaccagta aatgtattta agaaaccttc accatttaaa gcagaaccaa
     4981 ttaaagattt atctgatttt gtaacagaaa aattaacacc agatgttctt gctattgcaa
     5041 agttaccatc tacctttaat tcttcaccat ttttcaattc atactctaca atttcacttt
     5101 gaggtacaac acattccaga acgattacac cagtaccact taattctatt tggaagaagc
     5161 cttcaccacc aagtaaagca gatgagaagt tcttttgtgt aaatgctttt acttctattg
     5221 tatcagagca acaatagaat aatcctttat caacaattat agaatcatta tcaagactca
     5281 taaataaata atgtctaaaa gaaggttcca aataaacttc tccagtacct ttgtatacag
     5341 gtttcatcat tgattcatta gtaagtgctc ctgatattcc ttttttaaaa agacctccaa
     5401 caccaccaat cttagtttca gaagatatat ttcctttata atagtataat gcaccagctt
     5461 ctgtttttat tgatgaatta tttaatttta tcttaatttg tctagctttc aaaccagctt
     5521 ctctagcaaa atataatccc attgccatta atgggctaga agctccctca agtttttcaa
     5581 attctaatat ctcaaattcg cttttgccat ctgatgcatt ttctaaaatt tttacttttt
     5641 tgcttaaatc catacaaatt tctccccact atgataaaat ttatctctaa aatattataa
     5701 gatataactc tatattacat tttttttgaa aaaatcgcta ctttatcatc aaatttttac
     5761 atatttttaa gaagtttttg aaatgtttag atttaagtta agttatttaa ggatgtatag
     5821 ttatttcaat agacattttg agtattattt atcatttgtg ggtattatat tttatgggca
     5881 tgttaaaatt atttagaaga tagaagatag aagatagaag atagaagata gaagatagaa
     5941 gatagaagat agaagataga agatagaaga tagaagatag aagatagaag atagaagata
     6001 gaagatagaa gatagaagat agaagataga agatagaaga tagaagatag aagatttata
     6061 atatgtaata tttttgtttt gaaatttata aattagtatt actggttaga gttagtttag
     6121 tattaagtat attcgaagta gttttttagc tattttcata catttatatt acttaattat
     6181 atatagccta actaacttaa tatatcagtg aaatattgac taaaatgcct aaatatgtta
     6241 tcatacaagt tagtcaaata atttggcaac attaaatatt tagtaaattt ctaacattta
     6301 tggaacaaat ttctagcaaa gaaggtgaga tttatatggc tggtaaaaaa atattagcaa
     6361 caaacaaaag agcaagacat gaatatttta tagaagaaac ttatgaatgt ggaattgaat
     6421 taaaaggaac agaagtaaaa tcaataagac aaggcaaagt taacctaagt gatggatttg
     6481 catctgtaga taatagcgaa gtatttttaa aacaagttca tataagtcca tatgagcaag
     6541 gaaatatatt caatgttgat tctttaagag ttagaaaact attacttcat aagcatgaaa
     6601 taagaaagct tattggagca actactataa aggggtattc acttatacca cttagtttgt
     6661 atctaaaaaa cggtaaagtt aaagtagaga ttgccttagc aaaaggtaaa aaactatatg
     6721 ataaacgtca agatatggct aagaaagatg cacaaagacg tatagaaaga gaaatgacag
     6781 gtaagtatta aactagataa aactgtattt tttagaataa aattctttat tatctagaac
     6841 ataacattga tttttattct taaaagtatt atattatata tacattaaaa atttaataaa
     6901 aatggtgata aagtttgaga aaagtctact ggactataaa ctactcaagg gggcgtaaag
     6961 gtttcgacgt gggtttggaa cttgaggctg catgtcgtgt tactctgggt cacgtaaaaa
     7021 ctggggaact ttaaaataaa cgcagacgat aatttcgcga tagcagccta gttgctgctc
     7081 gtccctctta gtcctcctgc cgactaagac tggacgtcat tatgcaggga actactttag
     7141 gggtgtctcg acttgaagta gattaattgg gactggctaa gcatagagcc tgccacttgg
     7201 cgctatgtga ggtgagatat tagtgaagtt ggctaagcat gtagatgcag caaggaaaag
     7261 atttgcggac aggggttcga ctcccctcgt ctccaccatt gaaatccacg aaaacatatt
     7321 gatagtttag tcaatatgtt ttttttgtgc cttgaaatga tataattata atataaacta
     7381 atgtaaaatt taactaaaag gagatagtat aagtgagtat agataataat tttaaccatg
     7441 ttgcatttct gtggaatata gcagaaagtt taagaggtac atataaagaa gaagactata
     7501 gaaaagtaat gttaccacta atagtaataa gaagatttga ttgtctatta gatgattatg
     7561 atagagaaat aataaaatca gtatacgaag aatacgactt cttaccagaa gaagaaagag
     7621 atgagctagt aatagtagac ttaaaagaaa atcacgacat aaacttacaa ttttacaatg
     7681 taagtgattt tacatggaaa aaactattag atgatagtga aaatataaaa tcaaactttg
     7741 aagaatatct aaacggattc tcaaacaatg taaaagaaat aataggtaaa tttaaattta
     7801 aagatgaaat aaatcaatta gataaaaaag acaagctata tgcagttctt agcaaaatgt
     7861 atgaagtaga tctacatata aactcagtat ctaacaataa aatgggatac atatacgaag
     7921 aaatgttaag aagatttaca gaaaactcag cagcaggaga gcaatacaca ccaagagaag
     7981 ttataagact ttgtatggaa atactattta tgggtaaaga aaacttcctt acagaagaag
     8041 gaaaagtaat atctatagca gacttttgtt gtggtactgg aggaatgctt tcaatagctg
     8101 aagactatgt agaaaaaata aatccaagtg caatagtaaa tgtatatgga caagaactac
     8161 ttgatgaatc attcgcaata tgtcaggcag atatgctaat gaaagggcaa aatccagata
     8221 acataagact tggaaatact ttaacacaag atagatttaa cggagaaaaa ataagatttt
     8281 taatatcaaa tccgcctttt ggtgtaactt ggaaagacga agaaaagaaa gtaaaagaag
     8341 aagcagactt aggttttgat ggaagatttg gagcaggtac tccaagagta agtgatggtt
     8401 cattactttt cctacaaaat atgataagta aaatgtatga tgatgaagaa ggaagtagaa
     8461 tagctataat atttaacgga agtccattat ttacaggaga tgcaggaagt ggagaatcaa
     8521 acattagaaa atggataata gaaaatgatt tactagaagg tataatagca ctacctactg
     8581 atatgttcta caatacagga atagctacat atatttgggt acttactaac aaaaaagaag
     8641 ctaaaagaaa aggtaagata caacttgtaa atgctagtga atattatcaa ttaatgagaa
     8701 aaagcttagg aaacaaaaga aaagaaataa gtcttgaaca aatagaacaa ataaaagaaa
     8761 tatatgaaaa atttgaagaa agtgaaaata gtaaaatatt tgataacgaa gattttggat
     8821 atagaaaagt aacaatagaa agacctttaa aactaagctt taaagtaaac gaagaagcta
     8881 tagaaaatgt aaaaaatact actcaattta taaatcttgc agtttctaag aaaaaagata
     8941 tagaagttaa agaaaaagaa gaagctgaag gaaaaataaa acaagataag ttaataaaac
     9001 ttttagaaag ctttgacaat actttagagt atatggacag ggataaattt ataaaagatt
     9061 taaaagctaa atcaaaagag tttgatatac aactttcagc aggacttata aaggctatag
     9121 ttaatactat tggtgtaaga aatgaagatg cagaagtttg taaagatagc aaaggtaata
     9181 tagaaagtga tagttcatta aaagatactg aaagtatagg tcttaaagat gatgtatatg
     9241 agtattttga aaaggaagta aagcctcatg ttaatgatgc ttatatagat gaaagtagta
     9301 taaataatat aggatatgag ataccattta ctagacattt ttataagtat gaagagttaa
     9361 gaagttttgc tgagattatg aaggaagtag aaagtttaga aagtgagata gcactaaaga
     9421 taaggaaggt gctagggtaa tgggatacag atatagaagt gatgatgagt taaaggatag
     9481 tggagttgag tggttaggga agattcctaa ggattgggaa gtcagacaat taaaaagggt
     9541 actatcagat agaaaagaaa ataatgaacc tataaaaaca agtaatatac tgtcattgac
     9601 aatagaaaag ggagttattc catattctga aaaaggtact ggtggtaata aagctaaaga
     9661 agatttaaca caatacaaat tagtatatcc aaatgatatt gtattaaata gtatgaatgt
     9721 aattgttggt gcagtaggac tatcaaaata ttttgggtgt gcgagtcctg catattatat
     9781 gttatataaa ataaatgcta acgatgatat aagattttat aattatattt ttaaatctaa
     9841 attattccag gatagtttaa aaggattagg taatggaata atgatgaaac aatcagaaac
     9901 ttcaggtaag ttaaatacga tcagaatgag aattccaatg gaaaaactaa gttcagtaaa
     9961 gataccaatt gcttttaact tagatcaaga aaaaatagca aactttcttg atgaaaagac
    10021 ttcacagttt gattcaatta tatctaagaa agaaaagtta atagaaagat tagaagaagc
    10081 aaagaaaagc cttataagtg aggttgttac tggtaaggtt aaggttgtta agactgatga
    10141 tgggtatgag cttataaaga gaagtagtga agagatgaag gatagtggag ttgagtggtt
    10201 aggtgagata cctaaggaat gggatataaa aagacttaga tttttaggaa aactacaaaa
    10261 cggtataagt aaaagtagtg attcattcgg ttatggttat ccatttgtta gttatagtaa
    10321 tgtatataga aatatagaat taccacatat agtagaaggt ctagtaaatt caactgaaga
    10381 agaacgtaaa atttattcag tagaagaagg agatgtattc tttacaagaa cttcagaaac
    10441 agtagaagaa attggttttg cttctacgtg tatgaaaact attgataatt ctacttttgc
    10501 aggattttta ataaggttta gaccgaatga taaaagacta tacaaaggat tttctaaata
    10561 ttactttaga tgtgatttaa atagaaaata ttttgtaaaa gagatgaatt tagtaactag
    10621 agcatcttta tctcaaaatc ttttaaataa tctttctgtt gtattgccta agtatgaaga
    10681 acaagttgag atatataatg cacttgaatt aaaagttagc tacattaatt tatcgattaa
    10741 taagttaaag taccaaatag aaaaactaaa agaagctaaa caaagtttaa taagtgaagc
    10801 agtaacagga aaaatagaga tactagatta acttctagta tcttaatata gatatagatt
    10861 atacatacaa agttaaataa aagggagaat taatggataa aaatatattt caaattaata
    10921 taaatacaga tgtactgata agattaagga atcaaataaa tgaagaatat tatatatctt
    10981 atagaaaaga attttatgat aaaaataata aaaaacatcg cgcttgggat aaaatatgtg
    11041 caataatgga taggcttgac gatactgtag attatttaaa taaactcaaa ttaaatacag
    11101 gtaaatatag acgaagtgca tttgacttct ttgaatttat gaatcaggct tccgtagtaa
    11161 tggattgtat aacacatctt gcaaaaatat ttgaagtacc atatgaagaa gttgaaactt
    11221 caactgaaat ttttaatgag cttggaaaag agggtaaagg tacagataaa aaatactttg
    11281 aatatctacg ttcattgtgt tcagtccatc caatggaaac tagtagacac aaaatatacc
    11341 aagaaaatag ttttgaatgt agtccttatg tagtttggaa tgataaaagt atatggacta
    11401 atgatgctag cgatttatat gcagtggtat acacaagtaa agataatgat agtttcaaaa
    11461 gaattcatat atatatgaat caaatatttg agtatgtaaa aagaagagta aattttattg
    11521 aaaatataat agaacatata gaaaagtatc aacaagaaat aattgaaatt tttaggaata
    11581 ctccaataaa gaaagaagaa gattttgaaa gttatataga ttatttaaga aatctaaaaa
    11641 aagagataga aataagatat ggtaatgatt cttttcatga attaaactac cttatagaat
    11701 tatttgattt gccattatca aatcctgata atgaagagaa gatgaactta tataaaaatg
    11761 ctttaaaata tgctatcaaa tttgagcata atagtattca aaatatgaca aatgaaggtt
    11821 ataagaataa tggaatatta tatccacaag tgaatataac aactacatta ctagaagagc
    11881 taatttcatt aaatagtggt agtgaggaga gacgtaagta cagatatgca atagaaaaaa
    11941 catcatatct aagctatgat agttgttgga gtgatagaga atgggcatat gatcaaatag
    12001 atgatatgat gcctttttta gaaagatacg taacatttga taaagctcaa agtgattttg
    12061 agcattatgt attggtaaaa ctagcacttt atttagagtg cttagaaaat gaatgtttaa
    12121 taaataagaa cgttcctaat gataaaaagt ttcgtattaa ataattatag ctaataaaat
    12181 agggagaggg aggatattat gttaccaaaa gaaaaacacc tagaagaatc tattgaaaat
    12241 catttagttt caattggata tgaacaaggt cattcacaag aatttgacct tgaaaattgc
    12301 ttatttgtag atgatttatt tagattctta gaaaatactc aaaaagaatt actagatgag
    12361 tttaaataca acaggggaag taattacaaa aaagctttta tagatttatt aaattcaaac
    12421 atcaaaagaa gaggtcttat aagtgtactt agaaatggtg tagaagatat aatgatgaac
    12481 ggaaaatttc aattattcta caacaaacca aatctaacta ctaataaaac agcgtatgaa
    12541 aattataaac aaaatatatt caaaataaca agacaattaa aatatagctc aaagcacaac
    12601 aatactttag atatagtatt atctttaaat ggatttcccg ttatagtaat ggaacttaaa
    12661 aatcagttta ctaatcaaaa tgtgtataat gctataaagc aattcaaaaa agatagaaac
    12721 ccaaaagaaa ctttatttgg atttaatcaa agagtgttag tatattttgc agtagatact
    12781 gatgaagttt atatgacaac agagctaaaa ggagataaaa cattcttctt accgtttaat
    12841 aaaggtaaca atagaggtaa gggaaatcct gtagtagaag gaaaagaaaa aacttcttat
    12901 ctttgggaag aaatacttac taaagatagt ttacttgata ttataaaaag attttacttt
    12961 attcaagaag atgataaagg taatcaaaga gcaatatttc caagatttca tcaattagat
    13021 gcagtaagga agatagtaga agatttaaag ataaataaag taggtaaaaa ttatcttatc
    13081 caacatagtg ctggtagtgg taagactaac actatagcat ggacttcaca taggttatca
    13141 agtcttcatg atgaagaaaa taatgctata tttgactcta taattgtagt tacagacaga
    13201 aaggtacttg ataaacaatt acaaaatgct atatatcaat tagagcacaa aaacggactt
    13261 gttgttaaga tagatgaaga taaaaattca tcagacttag cagatgctat tgtaaataga
    13321 gcaaagatta ttataactac tattcaaaaa ttccagtatg cactacctaa gatagaacaa
    13381 cttgataaaa ggcgttatgc agtaattata gatgaagcac attcttcaac atctggtgaa
    13441 aatatgaatg cattaaaaga aactttagca ggaaagactt tagaagaagc taaggtaatt
    13501 gatgcggaaa atgaaaagaa tgaaaagtct agtatagata agatgaatga gcttatagag
    13561 aaaagaacaa atttaggaaa tataagtttc tttgctttta cagctactcc taagaataag
    13621 actatgcaag tatttgggag gataggtgaa gatggactac ctcatgaatt tcatttatat
    13681 tctatgaaac aagcgataga ggaaggattt atattagatg tactaaaaaa ctttatgcct
    13741 ataagtgttt attacaaagt aggaaagaaa attattgata accctgagtt tgataaggga
    13801 gaggctaaga gggctataaa taagtttgtt agtttaaatg agcacaatat aaggcaaaag
    13861 gttgaaacta tagtagatga ttttgtagat aataggtcta tgtggttaga aggtaaagct
    13921 aaaggtatga tagttactgc aagtagatta catgctgtta gatataaact agctatagac
    13981 caatatataa aagaaaaagg atatgatatg aaagcacttg ttgctttttc tggtactgta
    14041 aaagatggtg atgatgagta tacagaggta ggtatgaata aggaagtagc accagatatt
    14101 actgattcaa atttacctaa tatatttgat aaaaatgaat ttaagctatt aatagttgca
    14161 gaaaagtatc aaactggatt tgaccaacca aaactttgtg ctatgtatgt ggataaaaaa
    14221 ctagatggtg ttaaaactgt tcagacttta tcaaggctta atagaactta tcctaataaa
    14281 caaacattta tattagattt ccaaaatagt gtggaagaca tacaagatgc atataagcca
    14341 tattttgaaa tgactaatat tgataaggta acagatccaa atgtagtaaa tgacctttgg
    14401 tatcagttac atgaatatgc agtatatact gatgaggaag taaatgattt tacagtatta
    14461 ttctataaag agaaaagaac ttcttctcaa gatgctaaga tgaataattt aattgataag
    14521 gcagttgaaa ggtattgtta tttagaagaa gatgatgaaa aaaaatctga tgagtttaag
    14581 aaaaaatgtc aaaagtatat tactttttat aattttttaa ttcagattta tcctataaag
    14641 aacttaaatc ttttaagatt gcaagtttat ttagtagcac ttttaaagaa gttacctaaa
    14701 aagagtaaac aaagaataga ccttgatgat atgttaagcc ttgattatta taaattgcaa
    14761 aagcttggat tagatgataa aaaaggaacg gatatatcct tatataatgg agaaggtgag
    14821 ttagttggta tatctgaaac tgcgacttct cgtgtaagtg aagctgatga agagtattta
    14881 gaggatataa ttaaaaaaat aaatgatgtt tttggtgtag gacttacgga agatgataga
    14941 attatgataa atacttatga agagatgttt aagaatgaca aggatatcat ggatatagct
    15001 aaagctaata gttatgaaga tttagttagc acttttggta agaagtattt taagagaggt
    15061 atagttaaaa ccaaaaatag aaatgataga ttagttaagg agatattaac taataataat
    15121 ttacaaaatt atatgattca ttatttagct gaaaagatat atgaagaggc aaatagataa
    15181 gatacctata gagagctaat taactctcca taggtacttt attatgagct attgtatatt
    15241 attcatcttc taaaggtgta gcaattcctt ttaattctaa aaactttaag tctaaatcaa
    15301 gaataagctc tatagtatct ctatctatag aaacaccttc ttttttacac tcagtaagta
    15361 taaaatcaac ttcttcttca aactctataa atggtttatc gttattcata tttttgctcc
    15421 tttgattaaa tataatgtat ttactttaaa ttatatcaaa gaagagaaaa aaataacatt
    15481 atttgtaaaa aactatatat atataatata tagttttacc atatatagat aaactgtttt
    15541 taattaaatt ataaatatat attatattaa tgaatctata ttaaatagat agttgagtaa
    15601 gtagtaatac caaaaatatt aaaaagagat gttggtagaa atacgaacat ctctttttta
    15661 gtgcaaaaaa ttaaaaggag gaagttaata tgattaaatt atggaagaaa gaagatttaa
    15721 atttattatt agaatatcca aaggaagtag ttgaaaatgt tgataacgtc attaatattc
    15781 ttgatgaaaa ctatggatgt aacagaaaat taacagatga tggtggatat gtgtgtataa
    15841 ttgaggatat taaagaagta gaaaacttaa aatcaaatat attaaagggg ttagtagaag
    15901 agtttagtga tgtaatatat gaagatgaag ttaatacata taattcaaca ctatacttgt
    15961 tatctagtga ttatagtgtt actgtaattt ctaaaaatga agaaactgag tacttactta
    16021 aataagcata tacattgata aggtgtatat gcttatttta attttaataa ataaaggaga
    16081 aaagcagaat gaataaaaag agaactcctg ccaagagagt tgatatagtt ttacttaaat
    16141 tagtaaaaga acgtagtgtt ctatatgaaa ctagaaaaat aagtaatctt tatgatgctt
    16201 atagattagc aaagaatttt ttataaatag tgctagagaa aagtttgtag tggtatgttt
    16261 agatacaaag aattggccag tagcatagag gttgtaagta taggcactgt aaatttatct
    16321 atggtatatc caagagaaat atttaaagta gctatattaa ccaattcatc tgaaatgata
    16381 tgctttcaca atcacccaat gggtaatact gactttagca aggaagatag ttataactaa
    16441 tatgctacag aagcgtggag aaatattatg cattgaatta gtagatacat atagttattg
    16501 tagatgagga taaatacttt agctttaaag aaaactgtag gatataagag tcttagctta
    16561 aataaattag gctaaggctt tttctatgtt ccaaaacaat taaaaataaa tcaaatttag
    16621 gttaagcccg gaaagccctt gaaaaaaata atacatataa atttaaaact aaataggagt
    16681 tgtaacatgt ttaaaaaaga aaataggtat atgacaaaag agatagctga aaatctacca
    16741 attgaaatag ctatactact atggaattta atagatgact taactacaga aaaagattat
    16801 ctacaactat ttgaaataaa tcctatagga ttaggagtag ttgagatagt tcataagcaa
    16861 gaagtacctg aatatgaagc taggatatat atacagaatg ataaaattaa agataaatta
    16921 aaaatatatg ttatagataa tgttgaatat agaacaatga tgttttcaaa tgaatattag
    16981 ttaatgatta aaggagagag agtaatatga taaataataa atctgaaata attaataagt
    17041 tatttttcaa tgaattaaaa gagttaatag ataaatacaa caatatagat gataaaactg
    17101 tagatgtaat agaaaagata tttacatgta tagaagatga agaaataaag aattacctaa
    17161 taagagataa ttgtaagcta caagagctag taaatacata caataaactt gaagatttaa
    17221 acatagatga agctatattc tttgcttggt acaacttaac tataaataca atatctatag
    17281 ataaagcaag tgagtactac gatgatttaa ctacaagtaa ctatatagaa gtagaaaatc
    17341 atataatata tacaaatgaa agagatttaa ggaagtatgc tgaagatgaa ttagaaacta
    17401 tgttaagcat ggaacatcat atagatagat tactacataa agacatgtta atagatatgt
    17461 ggttaaacaa tacttcaaaa gaagaagtaa tagaagaaat attaatgagt gatgatatag
    17521 aagatatatt gaatttatct ccagaatatg catttacatt agctaatgga atagaatata
    17581 gatactcaga aaaagaagaa taaaataagt atatgtactg aaaaatacgg gctttaaggg
    17641 cttatacatt aaagtacata tttgaaaata ccatagattt tacaagagaa aatcagaatg
    17701 aaactttttc aaatactagt tattattaat tagtgctaga aaaggaggtg gagtgatgac
    17761 taacaacaat aataattgtg attataaggg catatatgca gatatggttg aggttttggg
    17821 tgaagaaata gtcattaaat tacataagta ttatcgtgga caacaaataa catttccaat
    17881 gaaactatat tctattgaat atgtggaacg atatatagtg aagaattata gaacaaaaac
    17941 aataaaagaa atgtgtagag agttaggata tacagaggga tggataaagc aattaattaa
    18001 taaaaataga ttaaattcaa agtaattaga gaaggagatt gacagtagat atgatattaa
    18061 cagtattagc aatacttatg gggacaggta tattttttct gattaattca atgctagata
    18121 taacgtattt tggatgtggc ccaataggat ttttatggtt tggatgtttt attgtgtcat
    18181 atgcaatatt aacatcatta ggtggtatag ttttaggatt gttaaagtgg atagtaatag
    18241 gattgattat actatttgta ataggattaa tagcgaataa gataaatgat taaattactg
    18301 tagattattt tgataaaata attatttaat atattgaggc attacatgat agaagaataa
    18361 atataaatga cttaaaatct ttaacttaac taaacaaaaa atatattatt tattacaaaa
    18421 aatgacaaaa aaattaaata ttatattgta ttatgacaat ataaaacatt ggagtttata
    18481 aattaggagg aatattttat gcaagatttt gattttataa gtgagacaat aaagaaattt
    18541 caatccgatt tagaaaacat ggataaggta aatatacttg ttgtaggtaa aacaggagta
    18601 ggtaaaagta cattaataaa taatatattt agagaagaat tagcagaaac tggaattggt
    18661 agacctgtaa cacaacactt gaaaaaaata agcaaagaag gattaccaat aaatttatac
    18721 gatacaaagg gattagagtt agaagaagat gtgcaagaga ctataaaaaa agagatatta
    18781 gatgaaatag aaaattgtaa tagaaattca ttagaaagaa atgataaaag tgagttaatg
    18841 catgtatgtt ggtattgtat aaatacatca gtagctagat gtgaagaaac tgatataaaa
    18901 tggataaatg agttaagtga aaagatacca gtaatagttg tattgacaca aggattccca
    18961 agaaaaaaag caagagaatt aaaatctagt attgatgatt taaatttaaa ctgtaagaat
    19021 gtaattccag ttttggcaga gcaactagta gaagaagagg atgagatagt tataaagtca
    19081 tatggtttag ataaattagt agaagttaca tatcaagtaa taccaaaaga ggtcaagaaa
    19141 gcttttgtta atgtacaaaa ggtagatata aaaaaaaagg ttgaagagtc gagaaaatgg
    19201 gttttaggat atgtaggagg atcaggagca ataggatttt caccaatacc attttcagat
    19261 gctccattat tagcaaccgc acaagttgct atgatatctc atataacagt aatatttggg
    19321 ttacaagttg ataaagcttt actaacggca attgtaagtt ctataggagg ggttacatca
    19381 gctactttac taggaaaaac attggtatcg aatatactta agtttatacc aggagctgga
    19441 actatagcag gtggagcgat aagtggttct gtagcggcat taacaactac agcattagga
    19501 atggcatata taagtgtatt agaatatata ctaaatgaga atatgaaagg taatgaggtt
    19561 aataatactg aaatagctga aaaaatgaaa gatgcataca aagatcaatt aggaatgtta
    19621 tctaagaaat aattaattca agataaagta taagattaat tatagggaaa aagtaaaatt
    19681 taaaggtata tttggtaaaa cataaacaaa aatgataatg gcgtcaatat tatttgttgt
    19741 catatgtagt agcttagtat atatattatt gagtgatagg tttaatcata agttaattta
    19801 cttagagaaa gatatattag atttaagaaa taaaaatttg taactagcta ataaaggatc
    19861 agtatagaaa cagataaaca agaggataat gtagatatta aatctaaaaa cactaataat
    19921 gaagaatata aaacttcaaa actatttagg cactagatag agtatttgct ataatgaata
    19981 agctaataga gaaattaaaa cgtttatagt aagataacta attcaaatac atttagtgga
    20041 cttcaaggaa catttgatac actatcaaac tatggtagct aattgataga ccaatcatct
    20101 aaattgaatt tatcaaatgt tggatggtta gaaatatctg atataattac aagtatagga
    20161 aaggactatt agattctgaa aatatgtcat ttgaagcatt aaatataatg ggaaaaatag
    20221 gaaaatttat aaattatatt gaaattaatt atattaacta attaaatatt gatacataat
    20281 gccttgctac agttaaatgt ggcaaggcat tatttttgta aagaaaggaa gaattaacat
    20341 ggcgaagcaa agtaaagagg aactatttga aaaagaaact aaatattgca agataataag
    20401 aagtgtaaca gtagaagatg gagatgaaag atttgcaata gagaaaatat atattaagtc
    20461 attacaaaga tacgaggtta gaatatgcct gtatcgtgac tgccgagata gaattaataa
    20521 acttattcca aggcctgtag atttaactat ggataagttt gtagcattaa tactggtagg
    20581 attaaaggct ggtgtactag atgatgattt caagcaagaa ctaattaaag gtttagctta
    20641 ggaggtagaa tatgacaata gaagagttta ctagtggagt aatgtttggg atttcaattt
    20701 ttagtgcagt attaaagcaa gtattagaaa ataaaaaata gtatacagga ggtatgagta
    20761 tgattggatt tataatagga agtataatag gaattggact atgtattagt atagagatac
    20821 tgatatcgaa aaatgccata atatactaag ttatatttaa attattaata gattcatata
    20881 taatttattt aagcccttaa agcccagata agttatatta aagtaaagtt tgtatttatg
    20941 taaattaaat aatatatatt attataaatt tgaaaggact ttataaatat gaaaggcaat
    21001 ataaacaaaa aagatataga tttaagtaaa ataacatttg ataagttttt agaagatgaa
    21061 ggattacaaa atagagagag agattactta tatgtagatg attatgacaa tactttagat
    21121 actcaagaat ttaaattaca tataattgaa gagaatactg ataacacata tatagataaa
    21181 gataaatact ggtatttaga tttagatgat agtttatttg aatacgaaga agatgaagat
    21241 actattgcta aaacattata tagtctaata gatattagaa ttaaattttt gaaacatgtt
    21301 gagtatagaa aatatataaa tctaggagta atagacgaag aatattttga tgagattaaa
    21361 tcatttttga aattttatat agtagcaaaa acaaagaaaa atgagctagg taattcaaat
    21421 ttaaagcaga tggctataga tatttttgat aaagaaaggt ttgaagacga catatcagat
    21481 gatgatatgg aaaatataag tgaatggata ggagaagatc aaaactttaa aatgttttat
    21541 gataaagttt gtagggatct ctcaaattct acacaggata aagaaatatt aaaattagca
    21601 ggaatcagga ataataaagt aaaacattac aaaggaagaa aaattagtag aaaatgtaaa
    21661 ttagaactta aatcattgac taaaatgtgg gagtatgaaa ctaaactgac tcatgatgca
    21721 aataaggcac ataagaaaat aaaatctaat atgcttgaaa aaatgacaga taagaattat
    21781 aaaaatatat atgaatattt aaataatgag attataaata gtaatgaaga atataaaaat
    21841 atttattttt atagcttaga attaaagtta gcgaatgaat taagaaagtg tactcttaaa
    21901 aacttaaata aaattaaaga catagaaaat caagtaaaat ttatttctta tactattgct
    21961 tatatacaca tagttccagt tttaacgata agagagaaac taagcgataa gttatttaat
    22021 atattgtcaa ctaatgaagg taaggatata gaaaaattta ctaaagaagt tagtcttata
    22081 atgagaatta taaatataga atgtttggag ttattaaata tagttagtaa gaacaactat
    22141 tataaaaatt ttagtgaaaa ggaattagag gagtttaaat ctatttataa tataaataat
    22201 ggaagaaagt catttgaaaa taaatataaa ataaaagtag attataatat agatgatgta
    22261 agaaagtatg gagaggttat aaaagcaata aatatagaaa gtaagaagaa aattgtagat
    22321 agaattaaga agaagagaaa atctaattag attttctcta ttttttttga aaaaaataaa
    22381 aaatatttgg tgaaaacgtt tatctttagg tgatatgagt agtcattatt tattatataa
    22441 tcatatttgt aggaacgagc taattaataa aatctaagaa caatagctta atgatgaaaa
    22501 agtacggcct ttcgggcttt gacaacagga ggtatattaa tgacaaactt taaaagtgta
    22561 aaggtagaat taattctact tattgaatgt gaagaaggaa atcgtaatga attagaaggt
    22621 atgattgatg aaggtgtatt aaatgaaaaa gaatatagtt taactatatt aggtagtaca
    22681 gagtatgaag ataacgcaag ggctatttac atacttatga atacaggagg atcatacgaa
    22741 aaaaacttac aacgattaag tagattacac ttgaaaattg aaaacttact taaagataca
    22801 aatgttaaat atagaggtat atctttagta ccgaatattg ttaaatggga taaataggag
    22861 gaatacaaat gagcaaggca ataaacagag aagaattaat taaattatat gaggaaatag
    22921 gattaaatgt tttagataat aacaccgtta tgaattcatc taataaaaag atatgtaatt
    22981 ttttagtaaa tataatagaa caaagggtta tagatggaaa aatgtattta tgtataaatg
    23041 ctatggggat taatggagag ctattatcag aggtaatatt agattcgaat accataaata
    23101 gaactacatg ggtgattgaa aattggggtt ttaaatattc tatagataaa aaatatatta
    23161 atgactttat tgaattgata caacacttat gtatgaatat aaaaactaag tatatatatg
    23221 accatacagg atggatatat caagacaata agtggatata tctacatgga ggaggagtta
    23281 taggaggttc agatgttcaa gttgagttag atgaatcaat agataactat atactaccag
    23341 ataaaattag tgacataaat aaagcatgtg aaatgagttt aaagctagta gacttaatag
    23401 aagatgataa aggtattata ttctcttcat tagtttacct atctccactt attgaaatta
    23461 tcagtagaaa aattaaacca ccggaaaata tagtatggac atatggaaaa acaggatcaa
    23521 gaaaaacagc agtatcaaaa gttgtattaa gtcattttgg tgattttaat aaaaaaattc
    23581 cagctacatt taatgatacg attacagcta ttgaattaaa agctaataag ttaaaggata
    23641 gcttatactt atgtgatgac tttgcaccta aacaagactt tagggataaa aaaattcaag
    23701 actcaaaggc agaatccatt ataagaatgt atggagatag agtagggaaa ggacgctcaa
    23761 gtaataagtt agatataaag gatgctaaga gacctagagg aatgatatta ataacaggtg
    23821 aagatgtgat atgtggagaa tcatctaatg ctagattatt atcaatagaa ttaaatagaa
    23881 gttcagtaga tttaaatata ctaactgaat tacagaataa tagtcatttg cttggagaag
    23941 caatgagagg atatatagaa tggttattat ctgaaatggg aaatattgat gaattatcta
    24001 atatattatt atataacttt aaatcttaca gggatgaaat tagagaacaa tataaagatg
    24061 atatacatgg aagaactatt gagtctagtg catggatatt agtaggtttt agttgtatgt
    24121 taaattatat tagagataaa ggtgttatag ataagtatga agatgatgag tacatggata
    24181 gagcgattaa agttgtagaa agattaatag aagagcagat aaaattaata tcgagaaact
    24241 cacctataga tatattttta aatacattaa aggaatcaat aaattcaaag tcaataagaa
    24301 tagcaacatt aaatgaccag aaccaagtta tagaagataa attagaatat atatgtggat
    24361 ataaggataa ggaatattat tacttttaca cagataaagt atattctttt atagaatttg
    24421 aaaataaaaa aagaggtata catttgggaa taagtcaaag acaactagtg aaacagttaa
    24481 gagatagtgg aattgtgaaa gttgattcta acaatgattc tcctaaaaaa actattcata
    24541 caaaagatgc tagcgattca acaggatata caacaataag accaagaatg cttcagatag
    24601 aaagaagtat aattgagaac tttcaaatat aacttataat caagagtgct tatgtactct
    24661 tgattatatt ctatgataaa tggaggatat aaatatgaca gaaacttata aaatcagcat
    24721 aggaagaaaa gatttatatt ctaaagacta taaggtagtt gaaagaactg actatgaaaa
    24781 gagacacaca gttaatataa gtagaagctt agtagacata ctttctatac ttgtaaaaga
    24841 agatagatag attaaaatat ggttacacac ttagtgttag tgtgtagcct ttaatataga
    24901 ttaaaattag tatatttaaa ctttggagga aataatatga caggaaagaa agcagtagta
    24961 tatgcacgct ttagttcaga taatcaaagg gacgaatcaa tagatgctca attaagagca
    25021 ataaatgaat atgcagataa gaataatata aaaatagtta atcaatttat agacagagca
    25081 aaatcagcaa catcagataa aaggccagag tttcaaaata tgattaaatt ctgtgaagca
    25141 gatacaacag gaatatctat ggtaatagtt cataagctag atagattttc tagagataag
    25201 tatgattcag ctatgtacaa acagaaatta aaagtaaaag gaataagagt tgtaagtgta
    25261 ttagagaact tagataattc gcctgaatcc ctaatattag aatcagttat agagggaatg
    25321 gcacaatatt acagtgctaa tttagcgagg gaagttgcta aaggtcagaa agaaaatgcc
    25381 cttaaagccc ttcataatgg aggcgatgca ccattaggtt atgatgtagc acatgataaa
    25441 acatatacag ttaatgaaga agaagctcaa gcagtaaaga ttatatttga tagatatatt
    25501 aaaggatatt catatagtaa gataatagat gaactaaata tcttaggata taagactaaa
    25561 agaggtaata aatttggtaa gaatagctta catggaatat taaataatga gaaatataca
    25621 ggagtatatg tatttaataa gactcagaga aaaggtgtag atggaaaaag aaatggacat
    25681 aagcaaaaga gtgaagagga aataataaaa atagaaggtg gtatgccaca aataatagac
    25741 aaaggagtat ttaaacaagc acaagatatg attcaaaaaa gaaagaaaac tccaggtgca
    25801 cataaagcta ctactacata tctattaaca ggactaatta aatgtggtga atgtggacat
    25861 gcatttcagg ggaataagag aaaagattct tatggaaatg aatatgtttc atatagatgt
    25921 ggatgtagaa agcaaaagag agaatgtaat aatagagaga taaaaagaga ctatttagaa
    25981 gagtttatat tacaagaact tgaaaagaac atattaaatg atgaagctat accgatttta
    26041 agtaaagcat tgaatgagaa gctgaatgat aagaatgaaa gtaatgcaga gctacttaaa
    26101 aatctagagc agaaactaga taaggtaaat aaagagataa gtaatatact aaatgctata
    26161 atgaatggta tagtaaatag catgttaaaa gataagcttg atgagctaga acaagtaaag
    26221 ttcaacttag atttaaaaat gaatgaacta aagatagaaa ataaagttgt agatggtgta
    26281 ggtgtaacag aagatcaaat aagaggtatg ttttcaagat ttaaggaatt tgtactagag
    26341 agaaatatac ctgaatgtaa gaagtttata ggagactata taaaggaagt aatcgtatat
    26401 aaagaccaca ttgaagtcat attcaatgtg gtcttttgtt ttgttgacaa tgatataact
    26461 catgatttac atattatcac agaaaggggt aaaattacat agtaaatata tattttataa
    26521 aagtaaatat cagatattat ttagataaaa attatttttg aaaatattag ttttaaaaaa
    26581 tatatttata taattataaa ataaaatggt ataataaaac tttaaatatg ataaagaggt
    26641 gagactattg gcagatatat ttacagtact aaattatcaa ggtagtaaaa agagattatt
    26701 agattttata tatgaaaata cacaagaata tattgattct gataaagctt ttttagatat
    26761 atgctcagga acgagtgcag tagcttataa tatgaaaaga cactttacgg tttatacaaa
    26821 tgatagtgaa aattatgcat atcagatatc taattcaatt gttaagaata atgaaagata
    26881 taactttagt caatcagatg aaatgatcat aagagaaaaa tataatatta atttacaaag
    26941 attaaataat atttttaacg atatggtaaa tcaggaagat atgttattag aacaagaaga
    27001 tgcagacggt ttaatagagc tatattcaaa ttgcccatgt atatggaaaa ataatgttga
    27061 tattgaaata gaaggcaata caatacagag tatatatgat ttaagaaaat gtaaacgaga
    27121 cataccgaat gtgttattca cattgtatta ttcaagtagt tacttcggca taaagcagtc
    27181 tatggagata gattctatta gatatgctat tgaatttata gaaagtgaaa taacaaagag
    27241 cattcttttt acaagtttat ttttcgcaat gaaagagtgt gtttttgcta aagatggtca
    27301 catggcacag ccattaaata tggaaacagg caagaaaaga atgttaagat taaaagcaag
    27361 aagtatatat gacatattta tagataaatt aaaggaattt aatactgagg agtttgtatt
    27421 atcacaaaag gagaataagt gcttcaataa agactttaga acattattag aaacagaaga
    27481 aagtataaaa gatgaagttg gttttatata tatagaccct ccgtatacag atatgcaata
    27541 ctcaagatat tatcatattc ttaatacagt tgttttgtat gattacccag aaatatcatt
    27601 gagtagagga actatatcaa aagggatata tagagaaggt agatatcaat cacctcttag
    27661 tcaaagaaaa catgcattaa atgagattga tttcttattt agtttctgta aggaaaataa
    27721 aataaatgta gcattaagtt atgcatatcc aatagataca gaaaaacaag caactaatag
    27781 atatacggct agtattgatg atttgataaa tgtagccaaa aaacattttg gagataatgt
    27841 taaggtagta catgaagaat atagtcatgc aaataataga aatagtgata aaaagaatgt
    27901 acttgaatat ttaataatgg gtatacacaa taaggagtaa atatggataa aattaataaa
    27961 ttaaaagaga ggatagctaa tatagaagcg acgaataaaa atgatatata ttcttctaac
    28021 ttatattggt cacaaaaacc attcaatata tgtgatgaaa ttattgaaca tttaagtgaa
    28081 gatggagata taatatttga tccgtttatg ggatctgggg ttacagttat agaggctttg
    28141 aaaaaagagt ataaaagaaa tgctattggg tgtgatataa atgatgcacc aatatttata
    28201 gtaaaaacat tattagaaaa atataatgtg cctaaaatag agaaagatat taaagagcta
    28261 aataataaat taagtaaatt atcaacttac tattcaacaa aatgtcgtga atgtggagaa
    28321 gtagttacta tatcgaaaac tatattcgat aaggagaata ggaatgcaaa gtgtgagcta
    28381 aagtctatac attataaatg taaggaatgt aaaaatgact ttaaaggtga accaaatgaa
    28441 actgaaatat ctaaattctt tattgatgaa gaattaaaga atatagatga ttttcaattc
    28501 atagccaatt caaggcttgc agttggcgaa ggtgatacag taagaactat tttttcaaat
    28561 agaaatttaa tagtattaga taaaatatta ggacatatag atgaatataa aaatagtgaa
    28621 tcttatgagc ctatgaagta tattttatta tctattttac atttaagtaa gattacagat
    28681 acacattcta attctcagtg gcctttatgg attcctaaaa aagactgtgt tgaaaaaaat
    28741 gtagtagaat tattcataaa gaatagtaag aaaaccatta aggcattaaa actagcatct
    28801 aaatatttat gtgaaaatag agaaaatgta gaactaatca aaaatctaga tagagggaaa
    28861 tttaaattga taaaagatgg agttcaaaat atagaagatg atgttatacc taatgaattt
    28921 gtggacttga taataacaga tccaccatat atgggacaag ttttatattc agagtatatg
    28981 cagttatata aaccttttat ggatttggaa ttcgaattag aaaatgaaat agttatatca
    29041 agtgccccag ctagagataa agggaaagta ttatattttg atatgatgaa ttctgcattt
    29101 gaaaatatat ctagagtatt aaaggtaggt aaatatatgt gtatgtactt ccatgatagt
    29161 aatctagatg tatgggataa tataatagat ataatgacca ataataattt gaaatacatg
    29221 ggacaaattc atgtaagtaa gaataaaaac actcttaaaa acatacttag tcctaagaag
    29281 tcgttaaatg gagattgtat aattttcttt aagaaagatt taaatataaa ctatgatata
    29341 aatgaagtag ataatataga agataagata aataatattg cacaagctat tatagatata
    29401 aaaggatacg catcaactcc agagttatat gataatggag cactagagta tataatatca
    29461 aacggaaaac taaaagagat ttctaaaaag tataaggatt taacaagtat atttgagcaa
    29521 agatttaatt gggataagga aagaggggta tggactgtat aataaataaa aagcatctat
    29581 agataattat agatgctttt tatttatatt ttatagtaaa ctaaatattt aataatttct
    29641 aatttttata caatatgtta aaggattggc tatccaaaga tattatttaa tataattttt
    29701 tagcattaac tctagttaaa tcacttttag ataatgaaac ctgtaatctt ctgataggag
    29761 aacccttttc taaacatcta ttgatctttt cctgtaactt cttacgaaga agaggaacta
    29821 catctgctgg ttcagttgag tagatatgtt caacttctat attttcttta tcaacaactg
    29881 ctaatattat agctttatca ttataatatt tatttagtac attatcagat atatcttgaa
    29941 agttccaagc atgattttta gaaactttat attcataagg attaccttca ctatcatagg
    30001 catcatgagc accttctcgt ccaccttgct ctgggtttac ttgatggttt aattctaatg
    30061 caactattaa ttcccaaaac ttattacttg taagtaaatt tttaactcct gttaattctt
    30121 caagttcact tgaagcttta taaatagcac ttagatattt atcataaaca ccattttgag
    30181 ctgatgggat tatcttactt gcatttaatc tagactctag ttgattaggg ctgacattta
    30241 tcatttttct ggaattttgt atactttgag tatactttag taaatattca cacattatat
    30301 ctccaggtat agagtaatac caatcaagct ttccagggta aggaaaatca acatcattta
    30361 tatgagcaaa tataactgcc ttagctttct ttaattttgc tatacttgta tccgaaaaat
    30421 cattaaacgt ccaagaatgg ttagagctac ttttcttgaa atgtttatat tcaaactcat
    30481 ttccgaattc atcttttgca tctctagaac cactatgtcc aggtattagt atgtgattaa
    30541 gtgaattagc agtcataact tcatatattt tgctattaga aagtatatca tttactttat
    30601 attctttaaa aataccatct tgtatagcct ttagttcatt tattttctgt gttattaaaa
    30661 catttgttat attatctctt atcatattat catagctcct taataaatta gtatttttag
    30721 ataagtcatt tttaattaga tttatcacat aactattgat gctatcattt ttaaatgcta
    30781 ctaaatccaa taaatcttta tgaaaatcat ccttgagtct tatagttagt tttttcaagc
    30841 tatcacctcg gatattaatt agatgtgccg tcgagataag tataattaag caggtttata
    30901 aagtcaagga ttaaaaaaat taaattatag atatataaaa gtatatatag caatatagat
    30961 aaatagctaa actattaatc gtgggtatta ttcttgcgat tcgtctccac caattttgaa
    31021 aaaagaatga tgaaatactt ggaaatacta gtctttgttg actagtattt tttattttgg
    31081 agaagaaatc caccaaccgt accccaacca cagaaaatga ggtttttttg ggggtatatt
    31141 ggggtgcatt taggctgtat aaaaagtttt tatttgttta ttattttatt tgtttattat
    31201 tttatttgtt tattatttta tttgtttatt atgataatag gtaacataga attttaatat
    31261 ctactttatg atataatatt tttataaatg taagacagtt taagttgtta attagtaaaa
    31321 atatatatta tataagcttg ataggagttg aggtttgtaa tggatgaaag aaaactagaa
    31381 ttttctattt tttgcattga agctttagca gaaaaattga atatagattc agaaggagtt
    31441 tataaacttc ttaaacatga tactgatatt ttagataact atataatacc ttgctatgaa
    31501 gcactacatt ctcaaagtaa aggttacata gtggaagatt taatgcaagt acttaaagaa
    31561 aagggtgtga tataatgatt gtatatcatg gttcatattg tgtggtaaag acgcctagta
    31621 ttaccttttc aagagattct ttagattttg gaaaaggatt ctatgtaacc agtataaaga
    31681 cacaagcaat taactggaca gagcgtttta aaaaaagagg taaaaaagca tatttaaata
    31741 gttattcttt agatattgat aaattaaaag aaaactgcaa ggtaaaaatc ttcgatagtt
    31801 ataatttaga atggcttgat tttataatag aatgtagaaa tatgagtgat atatatatga
    31861 aatatgacat tataataggt ggtattgctg atgataagat ttacaataca atagaactgt
    31921 atcaagataa tcttattgaa aaagatgaag ctttaaaaag attaaaatac tataaaccaa
    31981 atgaacaaat atgtataacc aatcaaaatg taatagataa atatttaaag tttaggtatg
    32041 aagaggaggt ataatgatta tggaagcaaa taaaacatta ttacaaatac tatataaaaa
    32101 aatcatatta gaattttcca aacaaacagg taagagcttg gaagaaagta tggattattt
    32161 atatacttct gaaacatata aacttataag tgagggtgta tcagatatgc actgtaaggg
    32221 tcatatatac ttggctcaag aattaatgtt agaaaatgga ttaatgtacc ataagggcta
    32281 tccaagataa ataattaaaa ctagatacga aataaggata caatataata taaaattaat
    32341 ttgtatcctt attttgattt tgtaattaac tatatgaaat cacaatattt atatgtattt
    32401 gatttttaca atgaaaatat tttatccaac ttttctacag cttcaacttt cttatcttcc
    32461 ataacttgag tatatatatt caaagttatt gatatatcat aatgacccat taaaacttgt
    32521 acagttttag gaggaacatc attctcaaaa agtctagtag cataagtttt cctaagacca
    32581 tgaaacttta taggctctat atttaagcta tttaatatag actttaaatt ccttaaaggt
    32641 cttttatcat ctatagggat gcctaattca ttgcaaaata cataattatt atttatatat
    32701 aattctccag cctttaatat atctgtattt tgatttactt tatgctcttt caatttattt
    32761 aaaacatctt ttggaatagg tacagttctg taagaatttg atgtctttgg cccttgctct
    32821 aatactttaa gctctctatt tccagtttta tctataaaat atgttctttg aagagttctt
    32881 ttaactgtta aattagactt tttaaaatca atatcaaacc atttaagtcc taataattct
    32941 cctaatctaa gtccagtacc aagagcaagt aaaaataata cctctaattt atgcccatct
    33001 atagctttta taaaactctt ttgttgttca agagtcaata tttcaagttt gttttctttt
    33061 ttctcaactg gtatatttac catagtacaa tagttacgtt gtataagacc ttgtttttca
    33121 gcttctgata agcaggtttt cagtttagtg tttatttgcc ttatagtagg tatactttta
    33181 ttggaatcaa gcaatctatt gtaatatttt tgtatgtgag atactcttaa gtactttaat
    33241 ttaacattgc ctaaatcagt atctttaata taattacgat agatgctttc atatctttca
    33301 aatgacttgg gttttaaatc ttttattcta tattcaaata accaattgta aaaccaatgc
    33361 tctaaagtta ttttatcatc tgaaatatca gagcttaaat aatattgttt tttataagca
    33421 tctaatttat ctttaacttc cttttgagtc ttaccataaa attcttttct cagtggttta
    33481 ccatcttcat tatagccaat cattatacgg gaacgccaac catctttata ttttccgata
    33541 gagccttctc cattagctct tttagttgcc atttttaaac agctccttat atgatttata
    33601 gttatatcca tcaaagaatg taatacattt ctctaataag aattttataa cttcatcagc
    33661 ttctagtgtt tcatagaagt ttagcattaa tgtgttgtac tcttgtttat gtttagctgg
    33721 tattgataga actcctatat cattatttat aagtattagg tttgaggtaa gtctagcagt
    33781 tcttttgttg ccatcataga agaattggca ataggacaac cataaattta ttattatagc
    33841 cttttctaat gggttagtta ttttatttat gatatttatg tcatttacaa ataaatcatt
    33901 taatagagtg gcttctatgc attcatattg agtagtacca gctataccta tattaccatt
    33961 tctgaaatct ccaacgatta gagcttcatc ttttgcaact attttatgta tagagcaaat
    34021 tgtatcttta tttaaagata agttttcttt gttacttaat tttattacat aatcccatga
    34081 tgattttata tttagtattt gattttggtc acttaattta tgtccgccta cggttatacc
    34141 atcaagtaat gtttgtactt ctgggaatgt gaaggggtta ttttctaggt tacccatatc
    34201 atatacaaat tcacttatca ttcttctaat gtatgttaaa gtatattttt tatctccatg
    34261 aggtatatta gtagggaatt tatgtgtgta ttttggtata tatgatttat tcatgatttt
    34321 agctcctttc atatctaatt tttatttatt aactatgaat gtataaaagt taataggtaa
    34381 actcatattt tgaaaatgtg caatactaat attcttcatc ctcatcttca ataagaccat
    34441 tagagttttc ttgtataaaa ttttcaacaa gaccatttat cacccaattt agatttttgg
    34501 agaagtcttt gaagtcagag aagtttaggt taattggttt tattgtatct gcattatctg
    34561 taatttcaaa ctttttttta tagtctgttt ttatatcata accataattt tctaatatgt
    34621 gaattactaa atccaactct ttatcaaata cttttaaatt aatattatta tttgttaaag
    34681 tatctaaatc attttcttca acaatgagtt tatttatagc aattgataaa gcagtagcta
    34741 tttttcttat catatctaaa ctaggcattc tttgattttg ctcatattta gctatagcat
    34801 gaactgaaca accaatcttg ttggctagct catcccttga catatttatc gattttctat
    34861 attgtttaat cttttctcca attgtattat tcatcataat ttcaccttaa atatatcatc
    34921 aaacaatgat tgtacttcta aacatgtaaa ggagttattt tctagattat cccatatcat
    34981 atactaaatt actcataact ctcttagtgt atgttagagt atatttttta tctccatgaa
    35041 ataaattagt aggggattta tatgtgtatt ttagcatata tgatttattc ataattgtaa
    35101 ctccattcat agttttttat cattgcttta gatagtattg atattatttt atattttagt
    35161 tagtagtttt tttatttctt taagtgtatt ttcaagcttt tcattttttt cctttagttc
    35221 gttattttct tgtaataatt taaagtaatc attattttta atagtaatat tttctttttg
    35281 agatattaat ttttttagtc gaatattact tccattatct gttgagtcac taaagtaatc
    35341 atagattgtt tctatacttt cttctgatag accaatatct aataattcat caattgattg
    35401 ttttgtaaga acatcatttt taataatttc ttttatgtca tcaggatgta atgaaaaatc
    35461 taattcagta ttagatatac aatctgaaag tatccaatta taaacttgat tttcatttag
    35521 ttctaatttt tcaccaagtt tataatattg gtctttatca gaccaggcac ctagcaataa
    35581 acttgaggta attctaatat catcttccat aagatttgaa atagcattta aaaattcatc
    35641 aaaagaatag tattcttctg ggaataaaga ctgtaaaagg tataaaacat cttcattagt
    35701 cattacttca ttttcaatat caaaataaaa gatagtcttt tcaattttca aagctttagc
    35761 gagttttgat aaggtatcta tactaggtat tcttttatca ttttcataat ttattagagc
    35821 atttcttgag atgccactaa tttcagcaag ttgaatctgc gttagctctt tttgttttct
    35881 tatctttttt aagttttttc caatttgcaa tattatcacc tcgtataaag tatatcccaa
    35941 aagtacacaa atgagaacag aaaaataata tattcttatt aacagtacac atatatcgac
    36001 attataatta aaatgtaaag tttacgaatg taaactagaa aaaaggagga aaaagtatgg
    36061 gaatcaattt aaaaatattt aggattaaaa aaggtctaaa gcaacaagaa ctggcagata
    36121 gagttggtat atcaagatat tatttatcaa acttggaaac aggtaaggct aataatccaa
    36181 gcaatgattt gatgattaaa ctatctagag ctttagatac aacagtagaa gaattatttt
    36241 ttagtaagga ggattaaaat atgaaagaga ataataattt agaggtttta aagacattac
    36301 aagccacttt aaacattatt gtaaagaatg aagaagaagc acaagaaaaa aagcaaggat
    36361 ataaaattat tgaaaatgcg aaaagaaata aagctgagct tataacatgt aaagaagcat
    36421 ctaagcttta tcctatagga gaatcaaaat ttaggcagtt atgtcattct aaaaacaaag
    36481 gattcccttg tatatggata aataatcgag tatacataat aaaaaataaa ttagatgatt
    36541 ggtttattga aaatgcaaat ggtatcaagt tatagtctta aagtaggtgt ttgcaattga
    36601 gcaataaagg gtggataaag ctttataggg agttgttgga taaatcaatt tggaaagata
    36661 ttaaaccaga aagaaaggta atattaataa ctctattact tatggctagt cataaagaaa
    36721 atcatgtttt tatagataat aaaaagataa aaataaagtt tggacaattt atttcttcat
    36781 atagcaaaat agctgaaaga tgtggtaaag gagttagtta tcaaatggtt agaagttgtt
    36841 tgttggtttt taaaaatgct ggttttctaa catacgaaac aacaaacaag tatatagtaa
    36901 taactattga aaactgggag ctttatcaag agaatataga aaatcaacaa cctaatcaac
    36961 agactattaa caatcagtca acaacaatca agaatataaa gaatgaaaat aatattatat
    37021 atagtgaaat cataagcaaa tttaactcaa cttgtattga actttcaaag gtcaagaaac
    37081 taacagatac aagaaagagg agaataaatt caagaataaa agaatatgat aaagaaactg
    37141 tattagaggt tataaatata gttagtaaaa caagttttct gaatggaaat aataatacag
    37201 gatggaaagc aaattttgat tggataatga attcaagtaa ttttactaag atactagagg
    37261 gaaattataa tagcaaggaa attgtcataa ataaagagac aggaatatat aatccagatg
    37321 actcacaatt aaagtggtaa ggaggttata ttataaataa ttcaaacaac ttaattaaaa
    37381 atacagaagc agagcaatca attaaattac actaagttag ttataaatct ttcatatatt
    37441 tgttttttaa gtatctctaa tatcgtttaa attacactaa gttagttgca gatataccag
    37501 aaaatatata taaaattatg cgtaaactat attgttatga gtacaaataa tatttaccta
    37561 ttcattaaaa aacttaacta agaatttaat ttatattatt gaagtatcaa taattttgga
    37621 aagtagaaaa ttatacccca ataatacccc aactacaaaa atagacaata aaaaataacc
    37681 agaaaaattg cttcaacatc atgaccaaat agccatatac ctatacttat aaaaaccaac
    37741 aaaacaatca aataaccact tataaattct cctcggtctc caccattgga atccacgaaa
    37801 acatattgat ttaatagtta atatgttttt tattttgaaa aatcatatat aatataaatt
    37861 aatagtttat ttaaaaattt cagtatgagc acaaaattat ataattattg ggaattagtt
    37921 ataataagta atttctagta agaatgattt gcaaaaaata ataatttaaa tacaaaaaac
    37981 taaaggatag aagtcataaa attaactttt aacctttagt tatataaata aaaaattagt
    38041 tattactaaa ttaaaattag atttttaaag aacccaatat ctctattatc catatcacca
    38101 attaacatgc ttctatcaag taataaatta tactcctcac agctagtttc aggcaataaa
    38161 gtacatgaat ggcatgctcc tagatttaaa gaatttctac cttgaccagt actatcaata
    38221 catattgggt cattagaaca ccaaagagca cgattaagag cactatttaa tatagatggt
    38281 aatgaatcat gcataccttg cctaactaat cctcctaaag taccttcaga gtcaccactt
    38341 gcagtgtaaa ctaaaattcc agacataact ttttcatttt cggaattatt acaatatata
    38401 cgttccctaa gtgaagaggt agcatatcca caaacaaaac taagttctct tataattaaa
    38461 tgagctaaag tatgtagtaa taagaattta ggtgtaattt ttctataagc tttgtcatta
    38521 acattaatat ctttattata tctagaattt aaaatagatg cattatcctg tattttagga
    38581 ttagatgaaa tccagctttt taccatatca ttatttatct ctatgaaaat accttcacct
    38641 ctaacctcag atgcaggata aaaagatttt ttacaatctc tagatcttaa acttataacc
    38701 ttagtatcat tacagctatc atctatatct tcttcaccaa atatattatt atcaggtgga
    38761 ttgactctac taaacccagt taatgccctt acttctctaa gtctttttac taacactatt
    38821 ttactgaagt ttggaatatc ataatcagat agattttgtt cttctattgt aaagtcatta
    38881 gtttttttag atttagcatc tatatttcca attaaagcct catattcttc ttctctatat
    38941 tgatttctag ttgttgttga tggtgaagaa tcttcaaaag gcaataaatc tttcaaaact
    39001 atggatactt tatcagtatc aaaacctgtt tctttagcaa tatcactggc aaagtcctca
    39061 aaatcctcat ccataaagcg ttctaactta ttctttttaa tagcacgatc taagaatttc
    39121 ttaaatgtat cataaccttt actagatagc actttagaat taagagtatc agagtatggt
    39181 ggtataataa ttgaactttc tatcttagga taatatacat tagaggcacc tctttgagaa
    39241 gcaaatgaat atttagtgca tttatttact tttttattat accaaggcga attaccagag
    39301 catttaaaca tttccttata ttcactaggt acaccataat cattagattt agaagatttt
    39361 aaacgcataa atgcgccctt catattagct ttagctccac agctacattc aagttctata
    39421 ccttctaatc ctaggttact ttttccagtc ttcattaata tttgaggatt ttcacaaatt
    39481 tcttttcttc cagatttatt tctaaaatga acccatttta cccatggaaa atcatcaata
    39541 tgaccatgtt cacaaatagt aattatacca gtaggtacta attgtttagt agactttttt
    39601 gtgctacaag taaggcaaat aggtattttc ataggtttat ctttatgagc atttttataa
    39661 agtttctccc aatcttctat aggcataaat tttctacatt ttggacaaaa gtaccatcta
    39721 ggaaacctaa caaaaggaac agaagatcta tcatctatag ttggaggtgt tcttagttca
    39781 ttaaccctca atgtagcctg aagcctatca tcttgtattt taattgtatt agtccaataa
    39841 tttggatgag cagtcattaa tgtttgatca ataaaatcta gcatagctcc tggtccaaaa
    39901 acacttgtag cttgatttat tcttaaatta tgagttttat catctaattt tatagacata
    39961 ttaattttcc tccaattcta taatttcaac agtacattgt ttatcaacat ttctcataga
    40021 atttaatgtt tgtttttcat ttgtaataac atccttatca taaggaatta aaagattatt
    40081 atatatttta ttatcaaatt tactagggcg cttataataa tgcaataaat tatctttatc
    40141 agatatttga gctttatcat gccataaatc cattataata tctaattcag atgaaatctc
    40201 agatttaaat tcatttacta tattatcatc agcattttga ttattttctc taaatatatc
    40261 gatagatcta tctattatga agttttttaa atcttctaca ccatccatat tttttgaaaa
    40321 tcgacttgca agatcattag cttgaagtcc taaaatatgt ctaatcatac ttattaatac
    40381 tgcatgcatg cctctttctc tagctggttc agagaaaggt gtaatactag taggttcaac
    40441 aaatttatag aatgattgat gataactaaa gaactgttca taatgagatc tatctcttgt
    40501 ttttgtagga ttatataatg taaatataag tccttcatta cttctaccaa ctctactgct
    40561 agcttgtatg tattcagatg ttaacttagg ctgaccaact actaccatta aatttaatct
    40621 atcaacatca acaccaactg aaatcatatt actagctaat agagtatcga cagttaaatt
    40681 attagaagta agagtattag atagttcatc taaagttgta tttatcttag tagaagtaag
    40741 tctactagta agctctcttt gttcatttag gttccttaat gattgataag ggtgggctga
    40801 gactctttta cataatcttt ccatgttgtc ttttatatcg tcatttaaaa gtgtgctaca
    40861 cttacctaat tctctgatac tattaaaata agatactagt gtccaatatt tatccaataa
    40921 attcaactct acattctcca tcattttaag tctttggagt aatgctgaca taagtctaac
    40981 ctctacagta acttgagttt ttccagatgg aataacacct acatatagtc ggccacgttt
    41041 tttacttaaa ggcatctctt taacaaagaa agagttacta gatgttaatc ctggaggtgg
    41101 aaactgagct acattacgat tatatagatt tttaacttga ttttgtgctt ttcttatagt
    41161 agcagtagaa gctataatct taggttttat acccttatag ctacacatag catcaagtgc
    41221 agtttcatat aaacctacca tagtacctaa aggtccagaa ataagatgaa gctcatcttg
    41281 aattattaac tcaggagata agttgctatt atcatcatct aaagcaaaaa gactagctac
    41341 ttctgtagtc caagttatca ttgcaaattt atcaactgta ccaaataaaa gtgttggagg
    41401 gttattataa atctcctcat ctacaacttg tataggtaac atatcttcaa atgaacaatc
    41461 ttcttcagga cagcataagt agaatttctt aggtctagat ttaattctat agccccaatc
    41521 accaacagct tttttagaag ttttatccca ttctttaaca agttttgttc cacaccaagg
    41581 acattttagt aattggaatt tattatattt tgattttaaa ttatctaact catattcact
    41641 atttatacta gatctaaact taatgttatt acctgttaag ttattaagca tttcttctgc
    41701 ctcatcatta aagtttggag tttgatcact cccaatccac aaaccaatag aaatctcttt
    41761 tttcccaagt tcagacttat tttcttttct aataatttca caggcacaaa ttaatatact
    41821 tgctcttatg aattgttgtg cagccaatag ccttagtgta tatctcatta tgatagctgt
    41881 accgttacca ttttctttgt ttcttattct tcttaagaag atagtaaaag cagatagacc
    41941 taagtaggcc tccgttttac ctccaccagt agggatccaa agaaggtcaa ctaaatctct
    42001 atgtttagag tcaggatttt ctatcgaaga taaacatgta agtatataag caagctggaa
    42061 tggtctccaa gcagaattat catttgagta atctatatag tcaaggttat tagatccagg
    42121 gagtctatcc gtaatatgta tagaatgaca tctttgcata agtatagctc tattcattag
    42181 agaaaatgct ctgtatactt catcattaga cttaattaaa tttatacctt ctagcattct
    42241 agacttagta tctctgcagt tttttatatg ctcatatgct acatctttaa aaacatcact
    42301 aagatctttt gaatcttctt ctagatcatc tatccaaata tcataagcat caacaaactt
    42361 ataaagaaaa tatattttct tatctttact aagatcagag atatctgata aatttttcat
    42421 agacagtaat tctctagaaa catcacttaa ttcatctaca ttaaagtcca atttaggtac
    42481 ttcaaaatga ggaataaact cagtacaaag ttttattaaa gcatcatttt gaatatcttg
    42541 atctatagct acaccatgac cagtagcaaa agttttttta tttcggtaaa gtaagtctaa
    42601 agatatttcc tcagaatcta actttttaaa agtatttact gatccagctg taaaaatatc
    42661 tataaactct aaatcattgt tatcttcagc taataccatt agttcaggct ggaaaatagt
    42721 attattatat ttcttaggcc cagattgact attaaccagc atacaagtaa tactaacaaa
    42781 gtcatttgaa taaattcttt tagataaaac tatatctaaa tctttagata taggtttttt
    42841 aatatagttt ttatccatat ctattgatac tattaagttt tcatgtggaa cacgaacata
    42901 accaccagtc ttattctcat catcagggca ttggttagct aacttgtata aaacaaattt
    42961 agcttcagca ttatcaagta aatcatgctt ttttagtaaa ttataaacct tctcagatgt
    43021 tagtttttgc ttaagatata ggaatgatcc cctaagttct acatattcat taattgtatt
    43081 gctagaatag aagtcgtcac cagtatattt aacatgacag tctgtatata aagcatttct
    43141 ataagtagca taatttaccc tgaaatttat tacttctgca tcttttttta ggaaaaaagt
    43201 catacccata gaagaaggta aagtttcatt agacatactt atagctctat ctagttcatt
    43261 ttgtgtatca tctgatgtaa atacattatc ttcaccatca tattcagatg tattacttac
    43321 atcaatattg ttattaagtg gatataaaat acctgttgaa taacgctcta ttggactatc
    43381 tgttattatt tcatatttta tattttcaca aagatcttcc gaaccaggtc caagaagttc
    43441 ttttcttaca agatctaggg ttttatctct tagttcacta caattcatat ttacctccta
    43501 tttatttaat aaacactata taagtctaaa actttgttaa caaggtgggg tgacagatac
    43561 ttatcacaac tgtgtatttc tcgtatttgt acaggtgtaa cataatcatt tgtattccca
    43621 agcatactta aaacaactgc agacacttca tctttaatta tatctgattt actgctaaag
    43681 aaattatcag atacaaatgt atctatagat ttttttattt tagtgtgcaa agagcgtata
    43741 cttttagtag catcatatac ctcggtatga tctaatttag aatctataac tttaaatatc
    43801 ttttcgaaag tttttaaata tataggacct attaatcttt tatttgttac ccagtttttg
    43861 attgttatag gattaatctt ttcacctaaa acagctaaac tgtttgatac atcattataa
    43921 ttatatttac tataagatat atagttttta agttctgatt tccatgaatt agttaattta
    43981 taatcatcaa gataatgaga tggcaataaa ttaagttcta atagcttttg tataacatta
    44041 ataactaatt caccctcttc atttaagtaa tcatcaacaa ataaaaacca gtcattaact
    44101 ttagcctctg aaagtttctt tttcctaaca ctttttttat cccaatctat tgaataggct
    44161 attgcctttt ttgtacagaa tatatactca ttattatcag aaatcatgaa tgatgtaaca
    44221 tgttcttttt tagagttgta aatcccttga tttatagata gagataatat aatttcattt
    44281 aaatttatat caaatttata attagatata tattcatcca tagaatcatc atcagaaata
    44341 ggactatcgg tagaataagc ttctaattta ttggtttcat aagttggttt aaaaggtttt
    44401 atatcaagta agttatatat tttattattt ttttcaattt tatttcttgc agaatttatt
    44461 tgattcatat agtaattata tttggataat tcactattat ataaatacag tgtaatattt
    44521 ataatcaaat cactatttaa aatatctaaa tatttcaaat catagaatcc aaacataata
    44581 agatttttta ttgtttttga atctttaaat ttttcagcag aaataacttg taaatttata
    44641 tttctagatt ttaataaatt tttaagtata atagcttcat attgtttttg tagcaatata
    44701 acaggtgtat ttggtgtaaa tgttttgtat ttatatgaag atactaattt atttagtgta
    44761 ttgaatttgg tattattatc agataattca tttataattc tttcaaattt gactataaca
    44821 tcatcaaata atgcacttaa ttctaaatct gggctagtac ttgaaattaa atattttata
    44881 ttttctagct tcgctgaagg agttgctata tttaaattta aaagctttat agtttcttct
    44941 aaaaaatcta ggttaaaagg tgtattttct aagattttaa gaagagagta actacacatc
    45001 ataagtgagt tttttatgga aatatcaaaa tcatacttag atatgtaata aagtgatttt
    45061 ctagcctgat gtatcaagtc agagatatta gaatcaacag aagtagtaca tatattttta
    45121 tttataagtt tatctagcca taatttctga aaagtagagg tattttcaaa atcttggcta
    45181 taggttaaca gaacatcttt agaccaagag tatatattta gattatctat atttggtaaa
    45241 tctaatatat tgcttatatt tgaatttgag gttacaatat ttacactttc tatccttttt
    45301 ctatttaaaa ttctttcaaa atcatttata tgcttataaa ttggataatc acctattata
    45361 aatacatttt ttatagactt atcagattta ataatttcaa atgcactaga aatattagtt
    45421 gtaaatttaa gtaaaggtat ttgcttagcg tgatttccag gataatcagt ttcggcacca
    45481 gttgatgaaa tatatgaaca tggaaatagt tcagtaaact tataagtatt attaatatct
    45541 tttattttaa tattactaac taagtcagat atatcatctt tacaaggcaa tataatcagt
    45601 gtagaatcat ttattactcc agtcatattt tttaagtctg aaaaacctaa agatgataga
    45661 acattctttg tttgaaaagt cttagatgct ggagttttgc ccatagaatt gacttctaaa
    45721 tgaccattat aggttataag catataagcc gagtcaatag gtataagtgt tgtatccgtt
    45781 ttatttttat gtcctaaagt tatgtgtgta ttatttatat ttttaaatat aactatttga
    45841 cccttgtaga gcaggggcat acctttatcg agttttgtaa gaatgtgatt atttgggttt
    45901 aatgtattat ttaagtatga gtttatagct atattcaaca aataaatagc atatatgtat
    45961 ttgtcattat aattgataat taagttatta tttttttcat ttataatata atttaataca
    46021 ttaatattat cttgataata cttagaattt gttagccttt gtttattaaa ataaatttca
    46081 catatatcat tatcaagaat ataatggata tttgtagtta acatttaaat ctcctttagt
    46141 agttactatt gtttattaga atagttttta ttctatatta taattatatt actaaaaaat
    46201 aatatatggt atataatata aaaaaagatt gataaataaa taaaaaatat attatgttat
    46261 ttatttgaat agattatcca gaagacataa ataagaaaaa taatgtgtaa gtaaaaatat
    46321 tagaatttat taaaagctat tacaagtata agaaatttga aatgattatt caaaaattga
    46381 catgtgaaaa aaaatatgat atgctgcaat aaaagttaat gtgaggtatg tcagaatgta
    46441 taatgttata gatctttttg caggtgcagg tggtttgagc ttaggatttg aaatgactaa
    46501 aaaatttaat atggtggctt ttgtagaaaa aaatgataac gcagcaaaaa cataccttga
    46561 aaatcatccg agtgttaagc gttattgtga tattaaaaga ttagattttc aagatatatt
    46621 aaacagtgtc gataagatag atgttgttat aggaggaccg ccatgtcaag gtttttctaa
    46681 tgctaataga caaaaaagaa aaattataaa tggaaataat gaattagtta agctatatgt
    46741 tgatgctatt gataaattaa agccaaatgt atttgtaatg gaaaatgtca aaactatatc
    46801 ttcaaataag cattcatttt atttgactaa aaaagataaa aatcatataa taaataattt
    46861 aaaattaaat atttataata aagatagtgt tttatatgat agaactgagt atataaatga
    46921 attatttaat attattagta tagaagatat tgaatcatat attatatcaa atatagactt
    46981 aaaaaatttg aaaataatta ttaagaaaaa aaaggatata tgtgattatt taaataaaat
    47041 atctaattat aagaaagtaa aatctaccat agaaaaattg gaaacaaggg gaaaaatgcc
    47101 aaaatggtat ttagaattaa taaataaagt aaaaaatata ttaga
//
LOCUS       DAEPIN010000024        46507 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.024, whole genome shotgun sequence.
ACCESSION   DAEPIN010000024 DAEPIN010000000
VERSION     DAEPIN010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 46507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46507
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.024"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            complement(221..1471)
                     /locus_tag="KR250_001577"
     CDS             complement(221..1471)
                     /locus_tag="KR250_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HBH0067516.1"
                     /translation="MATIKPFKAIRPNKYIVDKVAALPYDVMNSKEARRIAEGNPYSF
                     LHIDKSEIDLDENIDLYDEKVYLKARENLDEFRKQEILVKDDKECIYIYKQIMDGRAQ
                     VGIVACISVDESLNGTIKKHEYTRPEKEIDRTKHIKYCDANTGTILVTYKHQRVIDDI
                     INDFMDNNEPLYDFITDDKIEHTVWKIDNDDIIDNLVDKFEKLDYLYIADGHHRTASA
                     ENVAKEMRAKNPNYTGKEEFNYFIAMIAPDENLMVLDYNRVIKDLNGLSEEEFINKIK
                     ENFELEEIEGKEKYKPDKKGTFGMYLGDKWYKMKANKNLLEIEDPVDSLDISILQDYV
                     IDAILGIDNPRVDKRIDFIGGIRGLEELEKRVNEDMKVAFAMYPTSIEDLIRVADANK
                     IMPAKSTWFEPKVRCGLFLHEINE"
     gene            complement(1504..2445)
                     /locus_tag="KR250_001578"
     CDS             complement(1504..2445)
                     /locus_tag="KR250_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HBH0067517.1"
                     /translation="MYNILVTDGIEKEAARKLRELDFNVIEQFYEKDVLGDKLKDVDV
                     LVVRSATKVTKDVIDKALEGKKLKLIVRGGVGLDNIDVKYAQANGIKVMNTPNASSIS
                     VAELTIGQLFVLARFINTANVTMRDGKWEKKKYKGTEINGKTLGLIGFGRIAKEVAKR
                     AELLGMNVIYTDIMGEAQGFNNYKFCDMEEVLENADFLSLHIPFDKNKGAVITEKEIN
                     KMKKGAYLINCARGGLVDEKDLLKALDEGKLSAAAIDVYEQEPTLNLDLVNHPRVSPT
                     PHIGASTVEAQERIGDEIVNVIQDFFLDFNNLIGVAL"
     gene            complement(2685..3761)
                     /locus_tag="KR250_001579"
     CDS             complement(2685..3761)
                     /locus_tag="KR250_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HBH0067518.1"
                     /translation="MSKKLFIPGPIDVKEEVLQKMATPMIGHRGKDASMLQKSISEKM
                     QKLFYTNNTILLSTSSGTGLMEGSIRSCTSKKAAVFSCGSFGDRWYKMAVANNVPADI
                     FKVELGEATTPEMVDKVLSTGEYDLITVTHNETSTGIRNPIEEIGEVVKKYEDVIYCV
                     DTVSSAGGIKVEVDKIGIDICITSVQKALGLPPGMSICTFSQKAIDRAKQVPFRGVYL
                     DLLAMYEYLIKKNYQYPSTPSLSHMFALDFQLDNILDEGLDNRFNRHEDMANLVRNWA
                     KKHFQIFTNENHLSNTLTVIENTQGISVSNLNSKLQERGFQIANGYGDLKEKTFRISH
                     MGDYTVEDVQELLDNIDDILGFNK"
     gene            complement(4134..4649)
                     /locus_tag="KR250_001580"
     CDS             complement(4134..4649)
                     /locus_tag="KR250_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HBH0067519.1"
                     /translation="MDFLAKYKVLKKQNSSKSCLVCGTQNELGLKADFYELENGELVS
                     ICNTKDWHQSYPGRVHGGMSAAILDETIGRAVSINDDQIWGVTVSLELKYKKPVPTDA
                     TIKVVGRITKENRKLFEGTGEIILPNGDIAVTATGKYMKMPIGQIAEGDFSNEEWFFE
                     ESKEKVEYIEL"
     gene            complement(5012..6082)
                     /gene="leuB"
                     /locus_tag="KR250_001581"
     CDS             complement(5012..6082)
                     /gene="leuB"
                     /locus_tag="KR250_001581"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HBH0067520.1"
                     /translation="MNCNIAVIKGDGVGPEIIDEGIKVLNKICCKFNHRFDCEYVLAG
                     GCAIDETGEPLPNKTVETCRKNEAVLLGSVGGPKWDKCKGDKRPESGLLKLRESLGLF
                     ANLRPATMYESIKEASPLRTDIVEKGIDFVVVRELTGGIYFGERGRKIIDGIENAYDV
                     EIYNENEIRRIGKRAFEIARNRNKKLISVDKANVLESSRLWRSIMEDLAKEFEDVELS
                     HMYVDNAAMQVVKDPSQFDVIVTNNIFGDIISDEASMITGSIGMLPSASLREDSFGMY
                     EPIHGSAPDIAGKDIVNPIATILSVSMMLRHSFNLEEEAKCIEDAVQSVLNKGYRTID
                     IYNGVGNVVGTREMGELIVNEI"
     gene            complement(6089..6580)
                     /gene="leuD"
                     /locus_tag="KR250_001582"
     CDS             complement(6089..6580)
                     /gene="leuD"
                     /locus_tag="KR250_001582"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HBH0067521.1"
                     /translation="MIANGSVFKFGDNIDTDVIIPARYLNIADYKELATHCMEDIDDK
                     FISKVKKGDIIVATKNFGCGSSREHAPIVIKESGVSCVIASTFARIFFRNSINIGLPI
                     LECEEAANNIDEGDNIEVDFSTGVIKNITKGKEYKAEPFPEFMQNIILNEGLINSIKA
                     NRG"
     gene            complement(6595..7872)
                     /gene="leuC"
                     /locus_tag="KR250_001583"
     CDS             complement(6595..7872)
                     /gene="leuC"
                     /locus_tag="KR250_001583"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HBH0067522.1"
                     /translation="MGMTMTQKILAKHAKLNEVKKGQLIEADLDVVLGNDITSPVAIR
                     EFEKLGIEDVYDKTKVVMVLDHFTPNKDIKSAEQCKFTRSFAKSKGVVNFFDVGDMGI
                     EHVLLPEKGIVTAGDVIIGADSHTCTYGALGAFSTGVGSTDMGAGMATGKCWFKVPGA
                     IKFVLKNKPNKWISGKDIILHIIGEIGVDGALYKSMEFCGDGVEYLSMDDRFTICNMA
                     IEAGAKNGIFPVDDKTMEYINSHKCSTMTKDVNIYEADEDAVYDEVYEIDLAELKETV
                     AFPHLPENTRTVDEIDKDIKIDQVVIGSCTNGRISDLEVVAEIMKGKKVADGVRVMIF
                     PGTQKVYLEAIEKGYITTFIEAGAAVSTPTCGPCLGGHMGILAAGEKSISTTNRNFVG
                     RMGHVDSEVYLASPAVAAASAITGKISKPSEII"
     gene            complement(7829..9571)
                     /gene="leuA"
                     /locus_tag="KR250_001584"
     CDS             complement(7829..9571)
                     /gene="leuA"
                     /locus_tag="KR250_001584"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HBH0067523.1"
                     /translation="MKCGKYKKYDKMQIVNRKWPDNEIFKAPIWCSVDLRDGNQSLPT
                     PMSVNEKVRMFKMLIDTGFKEIEVGFPSASNTEYTFLRKLIDENMIPDDVTIQVLTQS
                     RAHLIEKTFESIRGCKKAIIHLYNSTSVLQRDVVFNMSKQEIIDIAVEGAKLFNEEVK
                     KYPETEFTFEYSPESFTGTEMDYALEICEAVIDVWKPTPQKKVIINLPSTVEMATPNV
                     YADQIEWFCKNISCRDSIILSLHTHNDRGTCTAASELGLLAGADRLEGTLFGNGERTG
                     NMDIVNVGLNLYTQGIDPELDFSNIDKIIGIYEDCTKLMVHDRHPYAGNLVHCAFSGS
                     HQDAIRKGMIAMKNRDNDYWEVPYLPIDPHDIGREYKEIIRINSQSGKGGAVYIMETD
                     YGFMIPKNMHSDFGNVVKMESDRIGEELSSEAIFNLFKKEYIEVESPYKVKKYKIKSM
                     DELNYENDDSNDTNMIEMTARISYMGNEQRIVGIGNGPVDSFNNALKQCGMKDYKFRY
                     YWEHALEEGSHSRGVAYVGIEHNNEVYFGVSISENINTAAINALMNAINKSYIEEEIK
                     NGDDYDAENISQTC"
     misc_binding    complement(9695..9941)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(10089..10535)
                     /locus_tag="KR250_001585"
     CDS             complement(10089..10535)
                     /locus_tag="KR250_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0067524.1"
                     /translation="MSKNKFIYMNYYDNGYFKKGMTIYMVLALIILATIFIYSTTILY
                     ILCIITAVFLFKQGRDFSNKYEGKVYITLDNHSILINNQCIFSIKSKQSQKFNYKIIE
                     KIEVVKNILNIYTNESNYKIRLRALSLEDEKKLLNIIDEKMKKFKA"
     gene            complement(10556..11275)
                     /locus_tag="KR250_001586"
     CDS             complement(10556..11275)
                     /locus_tag="KR250_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HBH0067525.1"
                     /translation="MKKSIMDVHCHTLISGHAHSTFKENVEEASKKNIKYLGISDHGP
                     NMPGGPHPFYFYNLHLLPREVQGVKILRGIEGNIMDYNGNLDVQEDMLQHLDYIIASL
                     HRPCIASGTKEENTNAILKVMDKPKVKIIGHPDDSRYPLDYEPIVKKAKDKNILLEIN
                     NSSLSSNSHRTGTWENVSHMLTLCKTYGARVILGTDSHICYSIGEFENAEKVLEAVDF
                     PDELVINYHEDEIIEFFDINF"
     gene            complement(11393..12667)
                     /locus_tag="KR250_001587"
     CDS             complement(11393..12667)
                     /locus_tag="KR250_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HBH0067526.1"
                     /translation="MFIMENDLTMQELLDQQEQVFSKVKVGELTTGKITAVRNDEVQL
                     GLDYGFDGIIPISELNIEKNQYIEDIYHIGDEITAVITKVSQKDGTITLSKLQLDKRN
                     DFAELQKAYDEHRIITVNVEKNIDKGVFANYNTYTFFIPISQLDTKFITDTSKFVGLN
                     LEVYIKELDVRKNRLVASHRDVLQERINKEREERRAQIKAEKEAERARIKQEREEEKA
                     RIKAAKEDLFNSLEVGQKRDGKVTKIMPYGAFVDIGGIEGLAHINNLAWTRVESVEDV
                     VSEGQEVEVYVLDVNKETKKIALALKDINNDPWDLIAKEVQIDDVVNAKVLRIIEKGA
                     FVQIKEGVDAYLPISELSDTRVAKVTNVVNIGDEVKVKILDFKPKTKRMLVSIKEATR
                     EPEEDITEYLEVEESLGSIGELFKDKFKDLEV"
     gene            complement(12867..14234)
                     /locus_tag="KR250_001588"
     CDS             complement(12867..14234)
                     /locus_tag="KR250_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBH0067527.1"
                     /translation="MENSNTNLGSESVGKLLFKLATPAIIAQIVNVLYNIVDRIFIGR
                     MENGEVAMAGVGVAFPIIIIITACSYLIGMGGGPLAAIKMGEQNNDEAEKIMSNSFSV
                     LVILAILLTIGFKIGKEPLLWMFGASESTIGYSMDYLNIYLIGTVFVQISMGMNTFIN
                     TQGFATTGMMTVAIGALINIILDPIFIFGFNMGVKGAALATIIAQGVSAIWVLMFLFG
                     KKSILKIKKKYMIPKASIILPVLGLGISPFIMQSTESLVLIALNSKLQMYGGDLAVGS
                     MAIMSSIMQILMLPNMGVTQGAQPIISYNYGSGQLDRVKKTFKLCLLSCFTYSTILWL
                     LLMIFPAFFVSIFNKNPQLLSMTSWSIKIYFAGAFMFGIQIACQQTFLALGKATISLV
                     LALLRKIVLLIPLIFILPTFFNEKLFAVILAEPVADITAATITAISFFIFYKCFLSKP
                     KAIKE"
     gene            complement(14484..14948)
                     /locus_tag="KR250_001589"
     CDS             complement(14484..14948)
                     /locus_tag="KR250_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small multi-drug export protein"
                     /protein_id="HBH0067528.1"
                     /translation="MSLRIGGNSLGYLHILILSMVPVTELRGAIPIGIAMGLNPIWVY
                     VFSVIGSTLVSIPLILTFRHVLQFLRGKKLFKGIADVIDRKINSRMKKLKSVSIIGII
                     LFVGIPLPTTGTWTASAIASILKMRIKDAFMGVFLGNLLSGVIVSALSLHII"
     gene            complement(14953..15582)
                     /locus_tag="KR250_001590"
     CDS             complement(14953..15582)
                     /locus_tag="KR250_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HBH0067529.1"
                     /translation="MKKSVLLLDVDYTVINTDSMIDFFIYSLKNKTFKTIIKLPYIIF
                     ILFMYLIRIIPLKKAKEAIFYPIVDFSEEDLKKFFDDCIMKKINESMKKVIYKNKEED
                     NVIIMITASPYAYMKYFKYYGLADEVIGTEFFYENSRYKNKFIGENCKGIEKVKRIKA
                     VLGKLGIEIDYENSYAYSDSKSDLPMLSLTKNAFLVSKKDGEVIEQINS"
     gene            complement(15616..16503)
                     /locus_tag="KR250_001591"
     CDS             complement(15616..16503)
                     /locus_tag="KR250_001591"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HBH0067530.1"
                     /translation="MKQTNTSIRLLTKISDYIKLMRIKQYIKNFFVFSAIIFSNNILN
                     INLFLNTFIAFVCFCLMSSSVYALNDAIDMDKDKKHPKKCNRPVASGRISKKSANILF
                     VVLALISVCLSTVVSFNLSIILSLYLINNILYSLKLKNIILLDVFSISLGFILRVYAG
                     CVAISVSLSNWIILCTFFLSLYLALGKRKKEIETLRDDAVEHRKILEDYDIENLNQMM
                     IVILSSTIVCYALYSTSNPEKPHMIFTTIFVVYGVLRYNYIINTTNENNPTDIVLKDN
                     ALKINVILWIITCLIILIF"
     gene            complement(16532..17770)
                     /locus_tag="KR250_001592"
     CDS             complement(16532..17770)
                     /locus_tag="KR250_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0067531.1"
                     /translation="MDGDILMSKILKYKNEMFLFILVVITYLIITKIFFSKTTIFYDL
                     NNTYDVLLDTDTGVLFNLNVFAISQDNSKHILFSAIISIFAYPIYLFCTSIANPGTTD
                     FNSAYGFGLICLQIITSAMSITLVFNHIKKIKMQRLTLILLTMIMIFSFPQLFMTLNV
                     ERFIYSQFSLIFFIVIANKMKGKNSYLVELAAIPLFGITISNIYLYFFNMIFEFKLKI
                     GKMLKHLITFILMAYICVVSTKSYESFMNLGNVIQYDTKFISGEPILEKIAMIIERLL
                     YSVFYFPGASIKKGLFLQNGEVATIPVILTLLALCFCVLSVIENSEKRVPKLCMGIII
                     FNLTLHGIVGYNLVNSSIMAINFSFAVIILLAYFTKALRKNEKNMYNIFLSLLLVTIV
                     ISNVNGFIEILNIGIKSYPV"
     gene            complement(18154..19296)
                     /locus_tag="KR250_001593"
     CDS             complement(18154..19296)
                     /locus_tag="KR250_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBH0067532.1"
                     /translation="MRYVEYGKTGKMVSVVGYGGLRFDLEKSNQENADLVKYAYEKGI
                     NYFDTAPGYCDDRSEDIFGLAFRQMIKEGKTDFYVSTKEKPKACDTSEKAIEAVKKSI
                     ERLGVSKINFYHVWNIRKMEHYELAMRPGGQYEGLLKCKEEGLIDHIVFSSHQPGDEV
                     IDILSENKFEGVTMGINILNFPYRLKGAKYAVDNGYGVVAMNPLSGGTIPKYNKELSF
                     LSKDGESTIESALRFNIGISQISISLIGFNKKQDIDDACKIADENRIYSDEDIHDIET
                     RLNKNMNEICTGCGYCKVCPKGINTPAYMLFYNEKQMFKKSDEEMTKLVYGLGHWNYT
                     MNSKAKAKECISCGKCEVECTQHLPIIDRLKEIKKWEEDGANSVKV"
     gene            complement(19437..20303)
                     /locus_tag="KR250_001594"
     CDS             complement(19437..20303)
                     /locus_tag="KR250_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBH0067533.1"
                     /translation="MDNYKKVKQVLWIILFANFAVALLKIIIGNQIKSYSMTADGFHS
                     LSDGASNIVGLIGIFFASKPKDKNHPYGHKKFEIITSLFISGMLFVIAIKIILSAVLR
                     IANPVVPAITIESLIALIITLFINIFVCMYEYRIGTKLNSYVLISDSLHTRSDIFVSL
                     GVLVTLVGVKLGFPVIIESIVPIIISAFIIYSAYGIFRPSIGILVDRVAVDEDYIKEI
                     VFEFNEVRDVHNIRSRGSKSSIYIDMHVMVDPFISVEQSHDLTHKIEKQIQEEINENA
                     QVIVHIEPFYSF"
     gene            complement(20637..22184)
                     /locus_tag="KR250_001595"
     CDS             complement(20637..22184)
                     /locus_tag="KR250_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBH0067534.1"
                     /translation="MANFFNFILLQKDKIIELLIQHMSLTVTSILIAIIVGVPLGIII
                     SRISSLRKFVLGFVNLVQAVPSMALLGLLVPILGIGSKPAIFMVVVYSLLPIVKNTYI
                     GITSIDPVVLESAKGIGLTRNQTLFKIQFPLALPIIMGGVRISAVTAVGLMTLAAFIG
                     AGGLGYLVFSGVQTVNNNMILAGAIPSCIIALLVDYLFSKIEVAVTPKGLNPKAPKKN
                     YIALKVISVVLVVSMLFVVFSSSFSSKKDTITIGSKDYTEQLILGNVYAELVEKNTNL
                     KVKKNLNLGGSSVAFNAIKSGELDMYVDYTGTLLVNVMKHAPIKDADEAYNVVKDTME
                     KENQLTLLDPLGFNNTYTLAMMPETAEKYGINTISDLTKYGKEFTFSPTLEFENREDG
                     LVGLSRDYGLKFKDVKAMNGSLRYTALDNNESQVIDAFLTDGLIKKFNLKILEDDKNF
                     FVPYYAAPLVREDTLKKYPELEKVMNMLSGKINEETMRELNYQVDELGKSPEEVAHSF
                     LVKEGLV"
     gene            complement(22177..23313)
                     /locus_tag="KR250_001596"
     CDS             complement(22177..23313)
                     /locus_tag="KR250_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0067535.1"
                     /translation="MIEIRNVTKKIGNNVILDDISLVVETGTLVVLIGSSGCGKTTTL
                     KLINKLIKPTSGEIYINGKPISQENEIELRRKIGYVIQNTGLFPHLTIKENIELIPRL
                     KKEKSVEEIEKRTLQLLEMVGLDSDEFLNKYPSELSGGQQQRIGVARAIATDAEIILM
                     DEPFSALDPITRTSLQEQLFSLQDELKKTIIFVTHDMDEALKIADKICIMKDGRIAQY
                     DTPENILRKPANDFVKDFIGEDRVWDNPEYIKARDIMIKNPIAVNSTRTVTQGIEIMR
                     TSKVDSLLIIDRAKTLKGIVTVKDMKDIDDKSILLADIMSSEPLHVNEGDNLVEILNV
                     MNRNSVGYIPVISDENKLVGLITRSSLLSVLSEQFLEMEVSVLG"
     gene            complement(23576..24673)
                     /gene="dinB"
                     /locus_tag="KR250_001597"
     CDS             complement(23576..24673)
                     /gene="dinB"
                     /locus_tag="KR250_001597"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HBH0067536.1"
                     /translation="MKHYRKIIHIDMDAFYASIEQRDNKKLKGRPVIVGGNPQSRGVV
                     ATCSYEARKFGIHSAMPSAVAYNRCPYAVFVRPRFDVYKSVSEQIRDIFYRYTDLVEP
                     LSLDEAYLDVTKNKKNIDSSIEIAKQIKKDIFREVGLTSSAGVSYNKFLAKIASDLRK
                     PNGLTVITEENAQDFLDKLPVNKFFGVGKVTSNTLKNLGIKTGYDLRCLNLFELENIF
                     KKRGYELYKFARGIDDRPVEPNRVRKSVGAETTLSHNLDIDEEETRNILDELCEEVCH
                     RLKNSEKFGKTLTLKIKYEDFTKITRSLSLEHYIDEYNDIRSGVDNLLRNVDVNGKQI
                     RLLGVTISNLSDKKETYKDITLFEYMDSIQM"
     gene            complement(25063..26568)
                     /locus_tag="KR250_001598"
     CDS             complement(25063..26568)
                     /locus_tag="KR250_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003374392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HBH0067537.1"
                     /translation="MKVGFDHKKYLEEQSKYILERVNNFDKLYLEFGGKLMGDLHAKR
                     VLPGFDENAKIKVLQHMKEKVEVVICVYAGDIERNKIRGDFGITYDMEVLRLIDDLRG
                     YELDVNSVVITRFEGQPATTVFINKLERRGIKVYKHYPTKGYPSDVETIVSDEGYGAN
                     EYIETTKPIVVVTAPGPNSGKLGTCLSQLYHENKRGNVVGYSKFETFPVWNVPVKHPL
                     NIAYEAATVDLKDVNMIDSFHLETYGEMSVNYNRDLELFPVLKKIIEKITGKESIFQS
                     PTDMGVNRVGFGIIDDEVIRKASIEEIIRRYFKTACEYKKGQVDKGAYDRMKMIMEEL
                     NLKPEDRNVVLPARNYSNKLKESADKNDTCPVVALELEDGTILTGKGSDLMNGTAAVV
                     LNAIKHLANISDDMHLISPVILEPIINLKTKTLASRNVSLSCQEVLTALSICAVTNPT
                     AQFAMEKLALLKGCQAHSTTILNRDDEQTFRKLGIDVTCDPEYPSQNLYYS"
     gene            complement(26830..27180)
                     /locus_tag="KR250_001599"
     CDS             complement(26830..27180)
                     /locus_tag="KR250_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0067538.1"
                     /translation="MAIVDKIFNEIKLMVARLLLGKKYSEYEEKDLVYNTEEEVILIT
                     LKRLVFQGNVNEAEEILFDKAKSVNSENMQYIAIEFYTMLMEKTDEELEAMNFSKQEV
                     YQGIEDIRKVLNLE"
     gene            complement(27403..27594)
                     /locus_tag="KR250_001600"
     CDS             complement(27403..27594)
                     /locus_tag="KR250_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0067539.1"
                     /translation="MLDRFFGEGGFGGCAWWIIILFFLFLAFQDCWAEMDLCAWIPFL
                     ILLLILCECGDVFDGGCGC"
     gene            complement(27712..28626)
                     /locus_tag="KR250_001601"
     CDS             complement(27712..28626)
                     /locus_tag="KR250_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBH0067540.1"
                     /translation="MRKIVLKDILFLVTTLSIIVILLLVTNITTNSKIKSTNESYLKS
                     DKNDVSQNNIQSSTKNTAFKTTILSTGKSDCILVEIGNKVIMIDTGEDKNGKQIVDRL
                     KEKGINTLDYLILTHLDKDHIGGVDSVLSSVKVKNIIQANYKKDSKQYDEYIDSLKKA
                     DIEPVLLKDNMNIVINSAEINIHPASKSKYESSNDYSIITNISYGAHKFLFAGDAEEE
                     RLSEFINGNTLKYDFVKMPHHGRYDKLTETFLESISPQYAVITCSEKKEPEEGVLKIL
                     ERLNIKTFLTSNGEVVINSDGKTLSVNQ"
     gene            complement(28760..30256)
                     /locus_tag="KR250_001602"
     CDS             complement(28760..30256)
                     /locus_tag="KR250_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBH0067541.1"
                     /translation="MFEFETQLKRLKHEVLTAVARLSIEENLTKENIEKIPYEIIKGE
                     KARYRCCVYKEREVVLERAQLALSLTPNSKYGDINPDSVSLDTDEQIIYIVEAACDRC
                     PINEFTVTELCRGCLAHRCKEACKFGAISYINGRAYINHDLCKACGMCKSSCQYDAIS
                     EVVRPCKSVCPTGALDFNRNTMKAMIHEDNCINCGACMSACPFGAISDKSLIAPVAKK
                     LAKKENMYAIVAPAITGQIDSNITYGQLKNAIKSLGFVDMIEASCGADAVTVHESNEL
                     IERIKSGDNYMTNSCCPGFFKYIEKMFPDEKDRISSTVSPMVATGRLVKKIDSDAKVV
                     FIGPCTAKKSEATEKSLKDSVDYVLTFEELMALFEAFNIDLSKCSNEDINDGSIFGRG
                     FGASGGLAKSVQNYIAEKGLSFDFEPIKVSGPRQIKKTMTMAKLGRLEGNFIEGMMCE
                     EGCINGAGKFLSGVKAKNTFDRKNGQSTKKSVLSNDKIKEFEDVNLER"
     gene            complement(30536..32026)
                     /locus_tag="KR250_001603"
     CDS             complement(30536..32026)
                     /locus_tag="KR250_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBH0067542.1"
                     /translation="MFQFETQIHKLRHDVLTAIARLGKEDNLTREAMDNIQYEIIKGE
                     KPTYRCCVYKERAIISERVNVSMGLQPGQDKIDLDTMDIEEDEQIIYILEAACDSCPI
                     NEFTVTDICRGCLAHRCKEACKFGAITHVGGMAYINHELCKACGMCKKACQYDAISEV
                     VRPCKSVCPTNALGFDRENMKAMIHEDKCLNCGACMSACPFGAISDKSLIAPVARKLV
                     QKEKMYAVVAPAITGQVEANVTYGQIKNAIKSLGFVDVLEAACGADAVTVHESLEFAE
                     RMEKGEAFMTNSCCPGFVGYIEKTYPNHVGKISSTVSPMIASGRFIKSHEPDAKVVFI
                     GPCTAKKSEAAKKELKGAVDYVLTFEEIMALFEAFDIDLAKCSNEDIDDASIFGRGFG
                     AAGGLTKAIENYLAEKGIGVNFEPVKISGSREIKKTMTMANLGKLEGNFIEGMMCEGG
                     CINGSGKFLSGAKVKATFDRKNSQSTKKSVLANTKIKDFSNINLEK"
     gene            32493..32693
                     /locus_tag="KR250_001604"
     CDS             32493..32693
                     /locus_tag="KR250_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000809131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBH0067543.1"
                     /translation="MKNGIVKWFNNEKGFGFISVEGEDDVFVHFSAIQNDGYKTLEEG
                     EKVSFDITQGNRGPQAENVNRI"
     gene            complement(32859..33737)
                     /gene="speB"
                     /locus_tag="KR250_001605"
     CDS             complement(32859..33737)
                     /gene="speB"
                     /locus_tag="KR250_001605"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HBH0067544.1"
                     /translation="MKNNFYHMNTFMSMNKNYEESNLIVFGVGFDGTTSNRPGARFAS
                     SSMRKEFYGLETYSPFLDLDLEDYNICDYGDLEISVGSTEQVLKEIYQETYKIVRDSK
                     VPFMIGGEHLVTLPAFKAVHEKYNDIYVIHFDAHTDLREEYNNSKNSHATVIKRIWDI
                     VGDNKIFQFGIRSGTKEEFKFATEEKHTYMEIGGIDTFENIVNMLNGKNIYLTIDLDV
                     LDASVFPGTGTPEPGGVNYREFQEIFKIIKNSNINIVGCDIVELSPDYDTTGVSTVIA
                     CKILRELCLIISDKIK"
     gene            complement(33727..34578)
                     /gene="speE"
                     /locus_tag="KR250_001606"
     CDS             complement(33727..34578)
                     /gene="speE"
                     /locus_tag="KR250_001606"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HBH0067545.1"
                     /translation="MELWYTEEWTENVRFSIKVNKHLFEGKSQFQRIDVFDSDEFGKF
                     LTIDGLMMVTYKDEFIYHEMITHVPMATNLNIKKVLVIGGGDGGTVRELSRYPQIEKI
                     DMVEIDKMVVDVSKEYMDICSCKLDDKRVSLYFEDGVNFVKCAHDKSYDLIIVDSTDP
                     IGPGEGLFSTDFYKDCYRILTDDGILVNQSESPYFDFNAKEMKRANKKLKQIFPISEV
                     YQAHIPTYPSGHWLFGFASKKLNPVKNQDRNGWEKLSLKTKYYNSDIHLGSFMLPQYV
                     KEMLDEE"
     gene            complement(34608..35021)
                     /gene="speD"
                     /locus_tag="KR250_001607"
     CDS             complement(34608..35021)
                     /gene="speD"
                     /locus_tag="KR250_001607"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HBH0067546.1"
                     /translation="MKFETLGRHILVEYYNCDENVLKDPRLIENFMNDSALNAKATIV
                     DSVFHHFNPWGVSGAVIIAESHLTIHTWPEYGYAAADFFTCGDIDPWKSFELLEQLLK
                     SERSESTEIPRGLTTKIKKYSKKDLGKITHKPEAV"
     gene            complement(35108..36583)
                     /locus_tag="KR250_001608"
     CDS             complement(35108..36583)
                     /locus_tag="KR250_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HBH0067547.1"
                     /translation="MDSEFANAVELSYECAPLLESLKEYSEKDIACFDVPGHVKNRGV
                     AILNKYLGESLMKMDINSSPTMDNVSAPNGIIKNAQDLLAQAYMADEAFFITNGTTQA
                     IHAMILSVIKPGEKVLLPRNIHKSVINALILCGGIPIFIQPEFDEKLGISLNITLEKV
                     RTEIEKDCNIKALFFLNPTYYGVCADLESIIELCHKNNVLVLVDEAHGAHFPFHLDLP
                     PSAISLGADMVAVSIHKTGGALTQSSALLLNRDNVSFEKVLQSINMLQSTSASYLLMA
                     SIDGARVNLVENGEKQLSKALNLSRYAKSKLNKIDGIKVLSTEILKQKGVKFIDETKL
                     CINVKELNLTGFEVYDLLYKNFSIQVELGDSYNILALVSIGTNKSDIDRLVKALSIIA
                     KIYRKESTLNEFNMVQINPIIKLNPREAFYAPKESVEINSCIDRICGESIMAYPPGIP
                     IIAPGELITEEIMEYIIFLKNSNAYLTDVQDKNLDRILVIK"
     gene            complement(37221..37874)
                     /locus_tag="KR250_001609"
     CDS             complement(37221..37874)
                     /locus_tag="KR250_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBH0067548.1"
                     /translation="MKVDPLTTQVYDYISKKIQNGEYEANQRITESEICKSIGVSRTP
                     AREALTRLAGENLLEKIPNKGFVVKEFQEKEKLDTYSVIGVLDALAGSSALQNLTESD
                     LVKMEELTEMMAVSIKYKNYNSYLKLSNEFHDIYITKSDNQVLVNLLNSLRYNFMSKS
                     YTSDNEDELYKMLTYSNNQHIEVVKSMRENDLENVERILREHWKTIPIAEMKKEKLL"
     gene            complement(38119..39984)
                     /locus_tag="KR250_001610"
     CDS             complement(38119..39984)
                     /locus_tag="KR250_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HBH0067549.1"
                     /translation="MQSVIEYNSWDKNFKAPFGALKFDEELTICVKVNEGYNIKSISL
                     EINREEEMRTITLNEELDNDKLGKCFCGKIEKFDGTGVYFYYFKVDVEMDGQIKTLFY
                     GKNRDNGYSCEYNYSDINKYQITVYKDFKVPTWYKEGILYHIFVDRFNNGNRSGKVDN
                     PKKNSFIYGNWEDTPMYIKDSQGDVIRWDFHGGNLRGIINKLGYLKKLGVSILYLSPI
                     FEASSNHKYDTGDYKKIDPMFGDEDTFKELIDKAKEKGISIVLDGVFSHTGADSKYFN
                     MYGNYNSLGAYQSKESPYYSWYMFEEFPQKYKSWWDVKTLPNINELEHSYMDYIIYDN
                     DSVINKWVNMGIKGWRLDVADELPTKFIRELKKELKKADDDSILIGEVWEDASNKISY
                     GQRRSYLLGEELDSVMGYPFRNNMFSFLKGEINSYELCNRYMQIKENYPKESFRSNLN
                     LIGTHDVTRAKTELNDDVDLVKLAVAIQMTFEGVPYIYYGDEAGLCGGVDPDNRRTYP
                     WKNEDEDMLNFYRDVIKIRNKNKLLSSGNTEFIYTNNDSVFSFIRVNENNDRMLILVN
                     RSENVESISLCIESSFIEEIPIKYSLKNANSTIQIENNELKVDLDSKSFRIFRVN"
     gene            complement(39989..42409)
                     /locus_tag="KR250_001611"
     CDS             complement(39989..42409)
                     /locus_tag="KR250_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HBH0067550.1"
                     /translation="MVTISKKKFKQKFEVKMYSLYAQSIKEATDEQLLNVLCSLLKDE
                     IAKKWVATKLDKKKEVYYFSLEFLIGRQLKSNLLNLNIEEEVREGLSELGINLDDLIE
                     TEVDPALGNGGLGRLAACFLDSMASLNISGQGYGIRYKYGLFEQKFVNGYQVEVPDNW
                     LTEGRYAWETVRPNEATMVKFGGEVELIKEGSHLKVIHKNYLPVMAMPYDIPIIGYQN
                     QCINTLRLFKSEIPKRDFGELTSNALNYSGSYEEALKHKYYTEEISQVLYPDDSNYAG
                     KLLRLKQEYFFVSAGIQDIIRKYKKNKLNINNLFDKVAIHINDTHPTLCIPELMRILL
                     DEENLSWDEAWEITKKTVSYTNHTIMSEAMEKWPVSMMKELLPRIYMIIEEINRRYVE
                     ELNNKGYDQDRIKRMSIIDCDNINMANLCIVTSHSVNGVAKLHTHILETEVLKDFYQD
                     EPNKFNNKTNGIAHRRWLISSNPQLSNLITDLIGDSWKKDTLQLKNIEKFKNDSSVLQ
                     RLDDIKYNNKANLAKFIKDKYDLNVDPSSIFDVQVKRLHAYKRQLLNIFNVLHMYHEL
                     LDNPNLNLDPRTFIFGAKAAPGYYLAKCIIKFINSVASTINNDVRVKDKLKVVFLENY
                     GVSLAEIIIPAANVSEQISTTTKEASGTSNMKFMMNGAITLATLDGANVEICEQVGKE
                     NMFLFGLSAEQVLNYNKYGGYSSLDLYHSNMDIKRVVDDLINGFIPNLGEEGRSIYNS
                     LTTYNDEYFVLRDFENYGQAQADINRLYRDKEKWNKMSLVNIANSGFFSSDRTISEYA
                     KDIWFKRV"
     gene            complement(42423..43865)
                     /gene="glgA"
                     /locus_tag="KR250_001612"
     CDS             complement(42423..43865)
                     /gene="glgA"
                     /locus_tag="KR250_001612"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011053260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HBH0067551.1"
                     /translation="MKVFYVTAECWPFAKTGGLGDVSYALPKELKKEGVDVRVIMPKY
                     STIPSYLKDQLKEIAVFSVRVGWRNQYCGLLEMELDGVKFYFIDNEFYFRREDERKSI
                     YGYGDDAERYTFFTDAVLEAISRIDFYPDVIHINDWHTGMLPLILKERYATLEGYKNI
                     KTMYTIHNLQYQGVFDKHVLYDILDLPQKYFDNGDIEYYGSINFMKAGINFADKIITV
                     SPTYANEIQTSFYGEQLDGLLRKESGKLKGILNGIDYDLNDPAKDKDIFVHYDVDSID
                     KKVENKLRLQDILGLKKDSSIPLIGIVSRLVSQKGFDLIAYMMPELMREDLQIVVLGT
                     GEHQYQSMFNYYDSNFSDKVSARITFNASLAQQIYAASDMFLMPSLFEPCGIGQMLAM
                     RYGSLPIVRETGGLRDTVTPYNKFTGEGNGFSFKNYNAHEMFFCLKNAIKVFKDKEKW
                     IKLVENAMKTDNSWKKSAKEYIETYRDICD"
     gene            complement(43894..45024)
                     /gene="glgD"
                     /locus_tag="KR250_001613"
     CDS             complement(43894..45024)
                     /gene="glgD"
                     /locus_tag="KR250_001613"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HBH0067552.1"
                     /translation="MRNECLGIINLNKKGDPAINKLNYGRPIASTPIAGRYRIIDFAL
                     SNMINSGITKVGIFAKEKYRSLTDHIGSGKDWDLSRKKGGLSIFSPENTKYRNTYSHR
                     EGDIYTILANLDYIEKSEEEYILIAPSYMLCNLNYSQALEYHKKSHNDITIIYKNVNN
                     ANKDFAGNLTLNLDSNNRVINVGNNLGKFPRANICMETYIMKREDFVECIYNIVNKGN
                     YCYLEEFIIEEAENMKIGAYEYTGYLKCVNSVESYFEMSKDLLNLEVADELLYSERKI
                     FTKEKNESPTIYTDSAKVENSFIASGCLIEGTVKDSIIFRKVNVEKGTVIENSIVMQN
                     CVIKSNAKLYNAILDKNTSVSNGKELRGDEKYPLVVEKNTNI"
     gene            complement(45026..46186)
                     /locus_tag="KR250_001614"
     CDS             complement(45026..46186)
                     /locus_tag="KR250_001614"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HBH0067553.1"
                     /translation="MKKEMLAMILAGGQGSRLGVFTKRIAKPAVSFGGKYRIIDFVLS
                     NCSNSGIDTVGVLTQYRPLILNSHIGMGSHWDLDRINGGVYVLQPFMNEKEGNWYNGT
                     AHAIYQNMDFVDTYNPEYVLILSGDHIYKMDYSKMLKFHKEKGSKATIAVIEVPWDEA
                     SRFGIMNTNEDSSIYEFEEKPSEPKSNLASMGVYIFDWKMLRNYFKEAEKNPEINYDD
                     FGKNLIPKMLEDNVGMYAYPFKGYWRDVGTIQSLWDANMDIIKSPETLDLADPKWKIY
                     TNTMAMPPQYIGKNANVHRSMIADGCRILGEVGNSVLSHGVVVGKGSKVIDSVIMPNV
                     VIGENVTIEKAMIGECATINDNVQIKNVNNEINVVSEYENIEPRCVLIEGGL"
BASE COUNT        14949 a         8386 c         4750 g        18422 t
ORIGIN      
        1 acatttaaaa ccttataaat atacaaatac aattatatga attttcatcc tcaattatag
       61 catagaatat gactatctat atcctaaaat tatccatttc taaaaaaaag gtaactatta
      121 ttaagaatat caacttatag aatttatatt ccacatacga ttttcaatca aaaaaacaag
      181 ggctaaaaag tgaattgtgc actttttagc cctttattaa ttattcattt atttcatgta
      241 agaatagacc acatcttact ttaggttcaa accatgttga cttagctggc attatcttat
      301 ttgcatctgc tactcttatt aaatcttcaa tactagtagg atacattgca aatgctactt
      361 tcatatcttc attaactctt ttttctaatt cttcaagacc tctaattcca ccgataaaat
      421 caattctttt atctactcta ggattatcaa ttcctaaaat agcatcaata acatagtctt
      481 gtaatataga aatgtctaat gagtcaacag ggtcttctat ttctaataaa tttttatttg
      541 ctttcatttt ataccactta tcacctaggt acattccgaa agttcctttt ttgtcaggtt
      601 tatatttttc ttttccttct atttcttcta gttcaaaatt ttcttttatt ttatttataa
      661 attcttcctc gcttaaacca tttaagtctt tgataaccct attataatcc aaaaccatta
      721 aattctcatc tggtgcaatc attgctatga agtaattaaa ttcttctttt ccagtataat
      781 ttggattttt agctctcatt tctttggcta cattttctgc tgaagctgtc ctatgatgac
      841 catctgcaat gtatagataa tctaattttt caaatttatc tactaggtta tctattatat
      901 catcattatc aatcttccat actgtatgct ctatcttgtc atctgttatg aaatcatata
      961 aaggttcatt attatccata aaatcattga ttatatcatc tatcaccctt tgatgtttgt
     1021 aagttacaag tatagttccc gtattagcat cacagtattt tatgtgttta gttctatcaa
     1081 tttccttttc tggtcgagtg tactcatgtt tttttattgt tccattcaag ctttcatcaa
     1141 ctgatataca tgctactata ccaacttgag ctctaccatc catgatttgt ttatatatat
     1201 atatacactc tttgtcatct tttactagaa tttcttgttt tcgaaactca tctaaatttt
     1261 ctcgcgcttt taaataaact ttttcatcat ataaatctat attttcatct aaatcaattt
     1321 ctgatttatc tatatgcaaa aatgaatatg gattcccttc tgcaattctt ctagcttctt
     1381 tactattcat aacatcgtaa ggaagtgctg ctactttatc tactatatat ttatttggtc
     1441 taatcgcctt aaatggttta atcgttgcca ttactaattt cctccctaat tatacaaaat
     1501 attttataat gctaccccta ttagattgtt gaagtcaaga aagaaatctt gaatcacatt
     1561 tactatttcg tctcctattc tttcttgagc ttctactgta gatgccccaa tatgaggtgt
     1621 aggagaaact cttggatgat ttactaagtc taaatttaat gtaggttctt gttcataaac
     1681 atctatagca gctgctgata atttaccttc atctaatgct tttagtaaat ctttttcatc
     1741 aactaaaccg cctcttgcac agtttattaa ataagcccct ttcttcatct tatttatttc
     1801 tttttcagtt attactgccc ccttattctt atcaaatggt atgtgtaaag ataagaaatc
     1861 tgcattttct aaaacttctt ccatatcaca gaatttataa ttgttaaagc cttgcgcctc
     1921 acccattatg tcagtatata taacattcat acctaataat tctgctctct ttgcaacttc
     1981 ttttgcaatt cttccaaaac ctataagtcc aagtgtcttt ccattaattt ctgtaccttt
     2041 atactttttc ttctcccatt ttccatctct catagttaca tttgcagtat ttatgaatct
     2101 tgcaagaaca aataattgac ctattgtaag ctctgcaaca gatatactgc ttgcatttgg
     2161 agtattcata acttttatgc cattagcttg agcatattta acatctatat tatccagacc
     2221 tactccacct cttactatca acttcaactt tttaccctct aatgctttat ctattacatc
     2281 tttagtaact ttagtagctg aacgtactac aagtacatca acatctttaa gtttatctcc
     2341 taaaacatct ttttcataaa actgctctat cacattaaaa tctaactctc tcaattttct
     2401 cgctgcttct ttctctattc catcagtaac taatatatta tacataacaa actcctccaa
     2461 tttttattca cttaattttt ccaattttta ttttttaaag tattttcatc aataaaaaac
     2521 tttacttaat ttaactttct gtttatataa ttttcaaaat atttttttat taaagtcata
     2581 tcaaattttc ctattgtttg ttaaaaattt aatatgtaat ttttataaaa tattatttat
     2641 tatatattct aaatttatct taaaacatac atatttttac aatattattt attaaaacct
     2701 aatatatcat caatattatc taataattct tgtacatctt cgacagtata gtcacccata
     2761 tgtgaaattc taaaagtttt ttcttttaaa tctccatagc catttgctat ttggaatcct
     2821 cgttcttgta attttgaatt taaatttgat acactaatac cttgagtatt ttctataaca
     2881 gtaagtgtat tggataagtg attttcattg gtaaaaattt ggaagtgttt tttagcccag
     2941 tttctaacta agtttgccat gtcttcatgt ctgttgaatc tgttatctag gccttcatct
     3001 aatatattat ctaattggaa atctaaagcg aacatgtgag aaagagatgg tgttgaaggg
     3061 tattggtagt ttttcttgat taagtattca tacattgcta acaaatcaag atatactcct
     3121 ctaaaaggta cttgcttagc tctatcgatt gctttttgag aaaatgtgca aatagacatt
     3181 cctggtggta atcccagtgc tttttgtact gatgttatac aaatatctat tccaatctta
     3241 tcaacctcga cttttattcc accggctgaa cttacagtat ctacacagta gattacatct
     3301 tcatactttt taacaacttc tccaatttct tctattggat ttcttattcc agttgaagtt
     3361 tcattgtgag ttacagttat taaatcgtat tctccggtag aaagcacctt atcaaccatt
     3421 tctggcgttg ttgcctctcc taattcaact ttaaatatat ccgctgggac attattagcc
     3481 actgccattt tgtaccatct atcaccaaag gatccacagg aaaatactgc tgcctttttg
     3541 gatgtacagg aacgaatgga gccttccatt aaaccagttc cggatgacgt ggataaaagt
     3601 atcgtgttgt ttgtatagaa tagtttttgc atcttttcac ttatactctt ttggagcata
     3661 gatgcatctt ttcctctatg tcctatcatt ggtgtagcca tcttttgcaa gacttcttct
     3721 ttaacatcaa taggaccagg aataaataat ttcttactca ttataattcc ccccttttta
     3781 gtttgtaata tggatagcgt ttttactgtc cttattttta ataactataa tatcaagctg
     3841 aattaactat gtcaacataa aatttatttt ttatatataa tttattttat atattttatg
     3901 aaaacattca ttttctacct tttcataagt ctgaattttc tgaattattc tctcaaaaaa
     3961 aatatacttt ttatctatat aaattaattt ttttagtatg taaatttgac gctattacga
     4021 taaatctttt tagtataact aatttatttt tatcttgtat ttttattgta tttttgaatt
     4081 ttattttttc taaatataaa atttatgttt taaaatgtaa aatttttatt taatcaaagt
     4141 tcaatatatt ccactttttc tttcgattct tcaaaaaacc attcttcatt agaaaaatca
     4201 ccttctgcaa tttgtccaat aggcattttc atatactttc cagttgctgt cactgctata
     4261 tctccatttg gtagaattat ttctcctgta ccttcaaata attttctgtt ttctttagtt
     4321 attcttccaa caacttttat agtagcatct gttggtactg gtttcttata tttcaactct
     4381 aaagatactg ttactcccca tatttggtca tcatttatac ttacagctct tccaatagtc
     4441 tcatccaata ttgcagcaga catacctcca tgaactctcc caggataact ttgatgccaa
     4501 tcctttgtat tacaaattga tacaagctct ccattttcaa gttcataaaa atctgcttta
     4561 agtcctagct cattttgtgt tccacaaact aaacaagatt tactactatt ttgcttcttc
     4621 aaaactttat actttgccaa aaaatccatc tcctaaccga cattagtagt cgcttaatat
     4681 taaataattc taaaatatat tatataatat taataaaatc cctgcaaact tctaataata
     4741 aaaataggct acttcaatat gataagttta atttaaagta ttattttcca aaaaataact
     4801 ttatcaaaat agaaactcaa agtagatata aacctctttt tcgttattaa aatagtaaaa
     4861 atatatttga cttaatataa aaaagagtgt ctcattaact aataaagttt attttgagac
     4921 acccctcata aaacaaatta tactacaata gatttttact aattctacaa gttatatatt
     4981 taaattatat tattcaaatt aaactataat tctaaatttc atttactatt aattcgccca
     5041 tttcccttgt tcctacaaca tttcccacac cattatagat atcaatagtt ctataacctt
     5101 tattgagtac actttgtaca gcatcttcaa tacacttagc ttcttcttct aagttaaaac
     5161 tatgtcttaa catcatgctt acagatagaa tagtcgcaat tggatttaca atatcttttc
     5221 cagctatatc aggtgcactt ccatgtattg gttcatacat accaaaacta tcttctctaa
     5281 gtgatgctga tggtaacata cctatagaac ccgtaatcat agatgcttca tcagatatta
     5341 tatctccaaa tatattatta gtcactatta catcaaattg gctagggtct tttacaactt
     5401 gcatagcagc attatcaaca tacatatgtg acagttctac atcttcaaac tctttagcta
     5461 agtcttccat tatacttctc caaagtcttg aactctcaag cacatttgcc ttatctacac
     5521 ttataagttt tttatttcta tttctggcta tttcaaaagc tctcttacca attcttctta
     5581 tttcattttc gttatatatt tctacatcat aagcattttc gataccatca atgatttttc
     5641 ttccacgttc tccaaaataa atgccccctg ttaattccct tacaacaaca aaatcaatgc
     5701 ctttctcaac tatatccgtt ctaagtggtg aagcttcttt tatactttca tacattgtag
     5761 ctggtcttaa atttgcaaat aaacccaatg actctcttaa ttttaaaaga ccagactctg
     5821 gtcttttatc tcccttacat ttatcccact ttggtcctcc aacagaacca agcaatacag
     5881 cttcgttttt tcgacaagtt tcaacagttt tattaggtag aggttctcca gtttcatcaa
     5941 tcgcgcatcc tccagctaat acatattcac aatcgaatct atgattaaat ttacaacaaa
     6001 ttttatttag aacctttata ccttcatcta taatttccgg tccaacacca tcacctttta
     6061 tgactgctat attacaattc atactccact accccctatt tgcttttatt gagttaatta
     6121 agccttcatt taatataata ttttgcataa actcaggaaa tggttctgct ttatattctt
     6181 ttccttttgt tatatttttt atcacacctg tagaaaaatc tacttctata ttatcccctt
     6241 catctatgtt attggcagct tcttcacatt caagtatagg aagccctata tttatagagt
     6301 ttctaaaaaa tattctagca aaagtagaag caatcacaca ggatactcca ctttctttta
     6361 ttacaattgg tgcatgttct cttgatgaac cacaaccaaa gtttttagtt gcaactataa
     6421 tatctccttt ttttacctta gaaataaatt tatcatcaat atcctccata caatgcgttg
     6481 ctagttcttt ataatcagct atatttaaat atctagctgg tattattaca tctgtatcta
     6541 tattgtcacc gaatttaaaa acacttccat tggcaatcat aaattttacc tcccttaaat
     6601 tatttctgat ggttttgata tttttccagt tatagctgat gctgctgcta cagctgggct
     6661 tgcaagatac acctctgaat caacatgccc cattcttcct acgaaattcc tatttgttgt
     6721 tgaaatggat ttttctcctg ctgcaagtat acccatatgt ccccctagac atggaccaca
     6781 agttggtgta gaaactgctg ctcctgcctc tataaaagtg gtaatatacc ctttttctat
     6841 agcttcaaga tatacttttt gagttccagg aaatatcata actctaactc catctgctac
     6901 ttttttgcct ttcattatct cagctactac ttcaaggtct gatattcttc catttgtaca
     6961 agagcctatt acaacttggt ctatctttat atccttatct atctcatcta ctgttctagt
     7021 attttcagga agatgaggaa aggctactgt ttcttttagt tcagctaaat ctatttcgta
     7081 tacttcatca tagactgcat cttcatctgc ttcatatata tttacatctt ttgtcatagt
     7141 tgaacactta tgtgaattta tatattccat agtcttatcg tctactggaa aaattccatt
     7201 ttttgctcct gcttctattg ccatattaca aatagtaaat ctatcatcca tagataagta
     7261 ttctactcca tctccacaaa attccattga cttgtaaagt gctccatcaa cacctatctc
     7321 ccctattata tgaagtatta tatctttacc acttatccat ttatttggtt tattcttcaa
     7381 aacaaatttt attgctccag gaaccttaaa ccaacatttt cctgtagcca tacctgctcc
     7441 catatcagtt gaacctacac cagtagaaaa tgctcctaat gctccatatg tgcaagtatg
     7501 agagtcagca cctataatta catctccagc agttactata cctttttcag gaagaagaac
     7561 atgctctatt cccatatctc ctacatcaaa aaaatttaca actccttttg attttgcgaa
     7621 acttcttgta aatttacatt gttctgcact ctttatatct ttattaggtg taaagtggtc
     7681 taaaaccatc actactttag tcttatcata aacatcttca attcctaatt tttcaaattc
     7741 tctaatggct actggtgatg ttatatcatt acctaaaact acatctaaat ctgcttcgat
     7801 taattgccct ttcttaactt catttaattt agcatgtttg gctaatattt tctgcgtcat
     7861 agtcatcccc attttttatt tcctcctcaa tataactttt atttattgca ttcataagtg
     7921 cattaatagc tgctgtattt atgttttccg atatacttac accaaaatag acttcattat
     7981 tgtgctctat tcctacataa gctacccctc ttgaatgtga cccttcctct aatgcatgtt
     8041 cccagtaata tctaaattta taatctttca tgccacattg ctttagtgca ttattaaatg
     8101 aatctacagg tccatttcct ataccaacaa tcctttgttc atttcccata taacttattc
     8161 ttgcagtcat ctcaatcata ttagtatcat tagaatcatc attttcatag tttaattcat
     8221 ccattgattt tattttatac ttcttaactt tataagggct ctcaacttca atatattctt
     8281 tcttaaacaa attaaatatt gcttcactac taagttcttc cccaattcta tcagattcca
     8341 ttttgactac atttccaaaa tcagaatgca tatttttagg tatcataaat ccataatctg
     8401 tttccattat ataaacagct cctccttttc cagattggct atttatcctt ataatttctt
     8461 tgtattctct tccaatatca tgagggtcta ttggtaaata tggaacttcc cagtaatcat
     8521 tatctctgtt tttcattgct atcatgcctt ttctaatagc atcttgatgt gaacctgaaa
     8581 aagcacaatg tacaagatta cctgcatatg gatgtctatc atgtaccata agttttgtac
     8641 agtcttcata tatacctatt atcttatcta tattgctaaa atctagctct gggtctattc
     8701 cttgtgtata aagattaagt cccacattta ctatatccat attcccagtt ctttcaccat
     8761 ttccaaatag tgttccttca agtctatcgg caccagccaa taatccaagt tcacttgcag
     8821 ccgtacaagt tcctctatca ttgtgagtat gcaaacttag tataatagaa tccctacaac
     8881 ttatattttt acaaaaccat tctatttggt ctgcatatac gtttggtgtt gccatttcaa
     8941 ctgttgatgg aagatttatt attacttttt tttgaggagt tggtttccaa acatctatca
     9001 ctgcctcaca tatttcaagt gcataatcca tctctgttcc tgtaaaactc tctggtgaat
     9061 attcaaaagt aaattctgtt tctggatatt tttttacttc ttcattaaat aattttgctc
     9121 cttcaactgc tatatctatt atttcttgtt tactcatatt aaaaaccaca tctctttgaa
     9181 gaacagatgt agagttatat aaatgtataa ttgctttttt acatcccctt attgattcaa
     9241 atgttttttc tataagatga gccctagatt gtgtaagtac ttgtatagtt acatcatctg
     9301 gaatcatatt ctcatctatt aattttctta aaaatgtata ctctgtattt gatgctgatg
     9361 gaaatcctac ctctatttct ttaaatcctg tatcaataag cattttgaac attctgactt
     9421 tttcatttac actcataggt gttggcagtg actgatttcc gtctcttaaa tcgacactac
     9481 accaaattgg agccttaaat atttcattat ctggccattt acgatttact atttgcattt
     9541 tatcatattt tttatacttt ccacacttca tgttatttcc ccctttttct gttttttgta
     9601 ttcagtatac ctcatatcga cgcaatcctg cacaattcat aaggataaat gtcaatttta
     9661 tcattagaat aaaaaaaacc tcatctctaa taaaattaga gacgaggttt tgctcgcggt
     9721 accactctat ttagctttct aaaaagctcc cttagtaata gccaatacaa taattataat
     9781 gactatattt cctataacgt ggaattactc cgattattct aagatttaaa tcttagaaaa
     9841 cagctcctag acaagttcag aactatcaac tactaaatca caccatcctt tagctctctg
     9901 aaagtttcat tgttcttact acttctattc atcacttttt taatcattta aattttctaa
     9961 atattttgat ttaatagtta ttatatacac attaaaatgt attgtcaaca caaaaaataa
    10021 ttatctcaat tttttatttt tgataagcaa ttctataaaa catgctaact attgaaaaca
    10081 gtatgatatt atgccttaaa ctttttcatc ttttcatcta tgatattcaa taattttttt
    10141 tcatcttcca aactcaatgc ccttaatcta attttataat tagattcgtt agtgtatata
    10201 tttaatatat tttttactac ttctattttt tctattatct tataattaaa cttttgactt
    10261 tgtttagatt ttatactgaa tatacattga ttatttatta atattgaatg attatcgaga
    10321 gtaatatata cttttccctc atatttattt gaaaaatcac gaccttgttt aaatagaaaa
    10381 actgctgtta ttatacataa aatatataat attgtcgttg aatatataaa aattgtagct
    10441 aaaattataa gtgctaaaac catatatata gtcattcctt ttttaaaata accattgtca
    10501 taataattca tatagataaa cttattttta ctcaaatttt ttcactccca agattctaaa
    10561 aatttatatc aaaaaattct ataatttcat cttcatgata atttatgact aattcatctg
    10621 gaaaatctac tgcttctaaa actttttctg cattttcaaa ttctcctata gaataacata
    10681 tatgagagtc agtaccaaga attactcttg caccatatgt tttacacaat gtcaacatat
    10741 gacttacatt ttcccaagta cccgttctat gagagtttga acttaatgat gaattattta
    10801 tttctaaaag gatattttta tctttagctt tttttactat aggttcataa tctaaaggat
    10861 acctactgtc atctggatgt ccaattattt ttaccttagg tttatccata acttttaaaa
    10921 tagcatttgt attttcttct tttgtaccag aagctataca aggtctatgc aaacttgcga
    10981 ttatataatc caaatgttgc aacatatctt cttgaacatc taaattacca ttataatcca
    11041 ttatatttcc ttctattcct ctaagaatct ttacgccttg cacttcacgt ggaagcaaat
    11101 gcaaattata aaaatagaat ggatgtggtc ctcctggcat attaggtcca tggtctgata
    11161 ttcctaagta ttttatattc ttcttactcg cctcttcgac attttccttg aaagtactat
    11221 gtgcatgtcc acttattaga gtgtgacaat gtacatccat aatagacttt ttcattaaat
    11281 ttcctcctaa tattttacat aaattcgtga aaatagctaa agggctattt caaaactaaa
    11341 gctgtcataa tagacagtgt aacgttttaa aacagcccca ttttcaatta ttttacactt
    11401 ctaaatcttt aaatttatct ttaaataatt ctccaatact tcctaaagat tcctctactt
    11461 ctaggtattc tgttatatcc tcttctggct ctctagttgc ttcttttatt gaaaccaaca
    11521 ttctcttagt ctttggttta aaatcaagaa tcttaacttt aacctcatct ccaatattaa
    11581 caacattagt aactttagcc actctagtat cactcaattc agatattggt aaatacgcat
    11641 ctacaccttc tttaatttga acaaaagccc ctttttctat tattctaaga actttagcat
    11701 tgacaacatc atcaatttgt acttcttttg ctattaaatc ccaagggtca ttatttatgt
    11761 cttttaatgc caaggcaatt tttttagtct ctttatttac atccaacaca taaacttcta
    11821 cttcttgtcc ctctgaaaca acatcttcaa cactttcaac tcttgtccaa gctaaattat
    11881 ttatatgtgc taatccttca atcccaccaa tatccacaaa tgcaccataa ggcattatct
    11941 ttgtcacctt accatctctt ttttgtccaa cttcaagaga attaaataag tcttctttag
    12001 ctgctttgat acgagctttt tcttcttctc tttcttgctt aattcttgct ctttcagctt
    12061 ctttttctgc cttaatttga gctcttcttt cttctctctc tttatttata cgctcttgta
    12121 atacatctct atgagatgct acaagtctat tttttctaac atccaattct tttatataaa
    12181 cttctaagtt aagaccaaca aatttagatg tatctgtaat aaattttgta tcaagttgag
    12241 aaattggtat aaaaaatgta tatgtattgt aattagcaaa tacaccttta tctatattct
    12301 tttcaacatt tacagttatt attctatgtt catcataagc cttttgtaac tcggcaaaat
    12361 catttctttt gtcaagttgt aatttagata aagttattgt accatctttt tgagatactt
    12421 ttgttattac agcagttatt tcatcaccta tgtggtatat atcttctata tattgatttt
    12481 tttctatgtt caattcactt ataggtatta ttccatcaaa cccatagtct aatcctaatt
    12541 gtacttcatc atttctcact gcagttattt ttccagttgt caattctcca actttcactt
    12601 tgctgaatac ttgttcttgc tggtcaagta attcttgcat tgttaaatca ttttccatta
    12661 taaacatcct ttcctcttat tgattatcat atctgtaaat attataccat taatattgca
    12721 aatacttaca tttttcttac tataattttt aaaataaatt caacttcata tatttataca
    12781 ttatagaaaa tatcttatta aattaaaata caaattctaa tttttatcta ataaaatatt
    12841 ttcataaaaa atttatattg tatatcttat tcttttattg ctttaggttt tgatagaaaa
    12901 catttataaa atataaagaa agaaattgct gttatagtag cagctgttat atctgcaact
    12961 ggctctgcta aaattactgc aaaaagtttt tcattaaaga atgttggaag tataaatatt
    13021 aaaggtatta aaagtacaat ctttctaagt agagctaaaa ccaaagatat tgttgctttt
    13081 ccaagtgcta aaaatgtctg ttgacaagct atttgaatcc caaacataaa agcaccagca
    13141 aaatatattt ttatactcca actagtcata ctaagtaact gcggattctt attaaatata
    13201 ctaacaaaaa atgcaggaaa tatcattaat aaaagccata aaatagtaga atatgtaaaa
    13261 catgacaaca aacacaactt aaatgtcttt tttactctat caagttgacc tgaaccataa
    13321 ttataactta ttattggttg tgctccttgt gtaaccccca tattaggaag cataagaatt
    13381 tgcattatac tgctcattat agccattgaa cctactgcta aatctcctcc atacatttgt
    13441 aatttactat ttaaggctat aagtactaaa ctttctgtag attgcattat aaatggagag
    13501 attcccaaac caagcactgg taagatgata cttgcttttg gtatcatata cttctttttt
    13561 attttaagta tacttttctt tccaaagaga aacataagta cccaaatagc agaaacgcct
    13621 tgtgcaatta tagtagctaa tgcagctcct tttacaccca tattaaagcc aaatataaaa
    13681 atcgggtcaa gtattatgtt tattaaagca cctatagcaa ctgtcatcat tccagttgta
    13741 gcaaaccctt gggtatttat aaaagtattc atacccattg aaatctgaac aaacactgta
    13801 cctattaaat aaatatttaa atagtccatt gaatatccaa ttgtagattc acttgctcca
    13861 aacatccaaa gtaaaggctc ttttccaatc ttaaaaccta tagtcaaaag tattgctaaa
    13921 atcaccagta cagaaaaact gttactcatt attttctctg cctcatcatt gttttgctca
    13981 cccattttta tagcagctaa tggacctcct cccataccga ttaaataact acatgcagtt
    14041 attataatta ttattggaaa tgccactcca acaccagcca tagcaacttc tccattttcc
    14101 attctaccaa taaaaatcct atcgactatg ttgtataaaa catttacaat ttgtgcgata
    14161 atagcaggtg tagcaagttt aaataataat ttacccacac tttcactacc taagttagtg
    14221 tttgaatttt ccattttaaa acctcctcaa tccaataatt aaaatgtatc actaaaattt
    14281 tattctatat aaaaatttca ttttattagc agacaaattt agagtacttt ccctacaagt
    14341 aatactatac tatatttctt ttgccatagt aaacatttta gaactaactg aagttaaata
    14401 ctttatctca ttttttattt attattaaaa atttttcaca catactcata aaaaaactat
    14461 ccaagtatat tacttagata gttttatatt atatgtaaag aaagagctga aactataacc
    14521 cctgataaca aattgccaag aaatactccc ataaaagcat cttttattct catttttaat
    14581 atggaagcta tagcagatgc agtccacgtt cctgtagttg gcagtggtat acctacaaat
    14641 agtattatac caattatcga aacacttttt aatttcttca ttcttgagtt tatttttcta
    14701 tcaattacat cagcaatccc tttaaatagt ttcttccctc ttaaaaattg taatacatgt
    14761 ctgaatgtca atatcaaggg aatcgatacc aatgtagaac ctattacact aaatacataa
    14821 acccatatag gatttaagcc cattgcaata cctataggaa tggctcccct caattctgtt
    14881 acaggaacca tagataaaat taatatgtgt aaatatccca aactattccc tcctattcta
    14941 agtgacatta agttatgaat ttatttgctc tataacctca ccatcctttt tactaactaa
    15001 aaatgcattt ttagtcaatg aaagcattgg taaatcactc ttagagtctg aatatgcata
    15061 tgaattttca taatcaatct ctatcccaag tttacctaat acagccttta ttctttttac
    15121 tttttcaatt cccttacagt tttctccaat gaatttattt ttatatcttg aattttcata
    15181 aaaaaactcc gtgcctataa cctcgtctgc aagaccataa tacttaaaat atttcatata
    15241 tgcatatgga gatgcagtta tcataattat aacattatct tcttctttat ttttgtaaat
    15301 aacctttttc atagattcgt taattttttt cattatgcaa tcatcaaaaa acttttttaa
    15361 atcttcttca ctaaaatcta ctattggata aaagattgcc tcttttgcct tcttcaatgg
    15421 tattattcta attaaataca taaatagaat aaaaataatg tatggtaact ttataatagt
    15481 tttaaaggtt ttatttttta aagagtaaat aaaaaaatct atcatgctat cagtatttat
    15541 aacagtataa tctacatcaa gtaataatac actttttttc ataagtattt ccccatccta
    15601 tcactaaaaa tatcactaaa aaatcaatat tattaaacat gtaattatcc ataagattac
    15661 gtttattttt aaagcattat cttttaagac aatatcagta ggattatttt catttgtagt
    15721 gttaattata taattatatc taagtacacc ataaactaca aatattgttg taaatatcat
    15781 atgtggcttt tctggattac ttgtagaata caatgcataa catacaattg tagaacttaa
    15841 tataactatc atcatttgat ttagattttc tatgtcataa tcttccaata tttttctatg
    15901 ttcaactgca tcatctctta gagtttctat ttctttttta cgttttccaa gagctaagta
    15961 cagagataag aaaaatgtac ataaaattat ccaatttgat aaactaacac ttatggctac
    16021 acatcctgca taaaccctaa gtataaaacc aagagatatt gaaaacacat ctaatagtat
    16081 tatattcttt agtttaagag aatataatat attgtttatc aaatagagac tgagaattat
    16141 agaaagatta aaactaacaa ctgttgataa acagactgaa attaaagcta atactacaaa
    16201 tagtatatta gcactttttt tgcttattct tccacttgcc actggtctat tacatttttt
    16261 aggatgtttt ttatctttat ccatgtctat agcatcattc agtgcatata ctgatgatga
    16321 cattagacaa aaacacacaa acgcaataaa cgtatttaaa aataaattta tatttagtat
    16381 gttgtttgaa aaaattatag ctgaaaatac aaagaaattc tttatatatt gctttattct
    16441 cattaacttt atataatctg aaattttagt aagaagcctt atacttgtat tggtctgttt
    16501 cataaactta ctaccctttc aatattattt tttaaacagg atatgatttt atccctatgt
    16561 ttaaaatttc tataaatcca ttgacattac tgattactat agtaaccaat aacagcgata
    16621 gaaatatatt atacatattc ttttcatttt ttctaagcgc tttcgtaaag taagccaata
    16681 aaattataac tgcaaaacta aaatttatag ccataatact tgaattgact agattatatc
    16741 ctactatacc atgtaaagtt agattgaata taattattcc catacatagc ttaggaactc
    16801 ttttttctga attttcaata actgataaaa cacaaaaaca taaagctaat aatgtaagaa
    16861 ttactggtat tgtagcaact tctccatttt gcaaaaataa ccctttttta atacttgctc
    16921 caggaaaata aaaaaccgaa tacaataatc tctctataat cattgcaatc ttttccaaaa
    16981 ttggttctcc acttataaat ttcgtatcat actgtattac atttccaagg ttcataaaac
    17041 tctcataaga ctttgttgaa actacacaaa tatatgccat taggataaat gtaattaaat
    17101 gttttaacat ttttcctatt tttaatttaa actcaaatat catattaaaa aaatacaaat
    17161 atatattact aatagttatt ccaaatagag gaatagctgc taattctact aaataagaat
    17221 tttttccttt catcttattt gcaataacta tgaaaaatat taatgaaaat tggctatata
    17281 taaatctctc tacatttaaa gtcataaata actgtggaaa agaaaatatc attatcatag
    17341 taagtaaaat taaagttaat ctctgcattt ttatcttttt aatatgatta aagactaaag
    17401 ttattgacat agcactagtt attatttgta gacaaattag tccaaaccca taggcactat
    17461 taaaatctgt tgtcccagga tttgctattg aagtacaaaa taggtatatt ggatatgcaa
    17521 aaattgatat aattgctgaa aacaatatgt gcttactatt gtcttgactt atagcaaata
    17581 catttaaatt gaataaaact cctgtatctg tatctaacaa tacatcataa gtattgttta
    17641 aatcataaaa tatagtagtc ttagaaaaaa atatcttagt tattattaag taagttatta
    17701 caactaatat aaacaaaaac atctcgtttt tatattttaa tattttactc atcaatatat
    17761 caccatccat tcattatata ggatttgctg acacctacaa tttattcctt atatttaata
    17821 gccatcttca taatgttctt acttgtttta ttgtaattga ataactcttt atttacaacc
    17881 acaatattta ctttattgat gttataatca gtcaagtatt gtttatataa catagattag
    17941 tgttgtataa aatgagaatt tgtaagcaat attaatgtaa aatatttgaa aaactttata
    18001 taaaaatata caatgttgct tatgtaatta aactataaca aattaaagca taatttaagg
    18061 agacaaaatt ataatctgtc tccttaaata ttaaaactat attttaaaac attactaaat
    18121 actatacaaa tttcaaatat agtataaaat taattagact ttaacactat tagctccatc
    18181 ttcttcccat tttttaattt cttttaatct atctattata ggtaaatgct gagtacattc
    18241 aacttcacat tttccacaag atatacattc tttagctttt gccttactat tcattgtgta
    18301 attccaatgt cctaagccat aaactaactt tgtcatttct tcatcactct ttttaaacat
    18361 ctgcttttca ttataaaaaa gcatataggc tggggtatta ataccttttg gacatacttt
    18421 acaataacca caacctgtac atatttcgtt catattctta tttaatctag tctcaatatc
    18481 atgtatatcc tcatctgaat aaattctatt ttcgtctgca attttacatg catcatctat
    18541 atcttgtttt ttattaaacc ctatcaaaga tatacttatt tgtgaaattc ctatgttgaa
    18601 tcttaaagct gattctatag tactttcgcc atcttttgat agaaaagaaa gttctttatt
    18661 atactttgga atagttccac cacttaatgg gttcattgcc acaacaccat atccattatc
    18721 aacagcatat ttggcacctt ttagcctata tggaaagttt aatatattta ttcccatagt
    18781 tacaccttca aacttatttt cacttagaat atctataacc tcatctcctg gttgatgaga
    18841 tgaaaatact atatggtcta tcaatccttc ttctttacat ttcaataatc cttcatattg
    18901 acctccaggt ctcattgcta gttcgtaatg ttccatcttt ctaatattcc aaacatgata
    18961 aaaatttatt ttagatacgc ctaatcgttc tattgatttt tttacagcct ctatagcttt
    19021 ttctgatgta tcacatgctt ttggcttttc tttagttgat acataaaaat cggtcttacc
    19081 ttcctttatc atttgtctaa aggctagtcc aaatatatct tcacttctgt catcacaata
    19141 ccctggtgct gtatcaaagt aattaatacc tttttcatac gcatatttga ctaaatcagc
    19201 attttcttga ttactttttt ctaaatcaaa ccttaatcct ccatagccaa ctactgaaac
    19261 cattttccct gtctttccat attcaacgta tctcattgta aattcctcct tagatatatt
    19321 aagacttaat ttctttatag aaaatattat accataaata atttttataa attatttatg
    19381 gtataatatt ttttttataa attatatttt tttataactt gtattttata actaagttaa
    19441 aaactataaa aaggttcaat atggactatt acttgagcat tttcattaat ttcttcttgt
    19501 atttgtttct caattttatg tgttaaatca tgagattgct ctacgcttat aaatgggtct
    19561 accattacat gcatgtcaat ataaatagaa cttttgctac ctctacttct gatattgtga
    19621 acatccctta cctcattaaa ttcaaacaca atttctttta tataatcctc atctacagcc
    19681 actctatcga ccaaaattcc aatagatggc ctaaatattc cataagccga ataaattatg
    19741 aatgcagaaa taataatagg aactatagat tctattatta ctggaaatcc taacttaaca
    19801 ccaactaaag taaccaatac acctagagac acaaatatat cacttcttgt atgcaatgaa
    19861 tccgaaatca aaacataact atttagttta gtcccaattc tatattcata catgcataca
    19921 aaaatattta taaaaagtgt aataattaaa gcaattaaac tttctatagt tatagcaggt
    19981 acaactggat tagctattct taacactgca ctaagaatta tcttaattgc tataacaaac
    20041 aacataccac ttataaataa actggttata atttcaaact ttttatgtcc ataaggatga
    20101 tttttatctt ttggtttaga tgcaaagaaa atacctataa gccccactat attagatgct
    20161 ccatctgaca aagaatgaaa tccgtctgct gtcatactat agctttttat ttgattacca
    20221 ataatgattt ttaataatgc tactgcaaaa ttagcaaata aaattatcca caatacttgc
    20281 ttcactttct tatagttatc cataacaaat tccccctccc ttcgattcaa taaagaaagc
    20341 tatctatcaa tatcaatcaa ccagacaata aaatcgtata taagcacatt tactatattt
    20401 atattcaaaa ttggttgtac ttgttcattc tatttgaaac ttaattataa ataaaaatcg
    20461 taggtatggt tttggtatac ataaaaataa gagactgtct caaaataaag ataatcttct
    20521 tttttattgt tgaaatgata aaatatattt gatttcatat aaaaaagagt gcctcatgaa
    20581 ctaaaaagtt cattttgaga cactccctta aatttttgat ataaactatt aattttttat
    20641 actaagcctt ctttaactaa gaatgaatga gcaacttctt ctggtgattt accaagttca
    20701 tccacttgat agttaagttc tctcatagtt tcttcattta tttttcctga aagcatattc
    20761 ataacttttt ctaactcagg atatttcttc aaagtatctt ctcttactaa tggtgctgca
    20821 taatatggta caaagaaatt tttatcgtcc tctaatatct taagattaaa ctttttaata
    20881 agaccatcag ttaaaaatgc atctataacc tgagattcat tattatctaa agctgtatat
    20941 cttaaacttc cattcattgc ttttacatct ttgaatttta aaccataatc tctagaaagc
    21001 cctacaagac catcttctct attttcaaac tccaaagttg gtgagaatgt aaattcttta
    21061 ccatatttag tcaaatcaga aatagtattt attccatact tttcagcagt ttcaggcatc
    21121 atagctaatg tgtaagtatt attaaatcca agagggtcaa gtaaagttaa ctgattttct
    21181 ttttccatag tatcttttac aacattatat gcttcatcag catctttaat cggagcatgt
    21241 ttcattacat ttaccaaaag tgtacctgta tagtcaacat acatgtctaa ctcaccagat
    21301 ttaattgcat taaacgcaac agatgaacca cctagattta aattcttctt aactttaaga
    21361 ttcgtatttt tttctactaa ttcagcataa acattgccaa gtataagttg ttctgtataa
    21421 tctttagaac caatagttat tgtatctttt ttacttgaaa aactagaact aaaaacaacg
    21481 aacaacattg aaacaacaag cactacagat attactttaa gtgctatata attcttctta
    21541 ggtgcttttg gatttaatcc ttttggagta actgcaactt caattttact aaataaataa
    21601 tcaactaaca gtgcaattat acatgatggg attgcccctg ctaaaatcat attattattt
    21661 acagtttgaa cccctgaaaa tactaaatat cccaaaccac cagcacctat aaatgctgca
    21721 agagtcatca aaccaacagc tgtaactgca gatattctaa ctccacccat tattatagga
    21781 agcgctaaag gaaattgtat tttaaataat gtctgatttc ttgtaagacc aataccttta
    21841 gctgattcta atactactgg gtcaatactt gttataccta tatacgtatt ttttacaatt
    21901 ggtagtaatg aataaaccac taccataaat atggctggtt tacttcctat accaagtata
    21961 ggtacaagaa gacccaaaag tgccatagac ggaactgctt ggactaagtt tacaaatcca
    22021 agtacaaact ttctaagaga tgagattctt gaaataatta ttccaagagg aacaccaact
    22081 attatagcta ttaaaataga tgtaacagtt agactcatat gttgtattaa aagctctata
    22141 atcttatctt tttgtaatag tataaagtta aaaaaattag ccaagaacac tcacctccat
    22201 ttctaagaat tgttcactta aaacagaaag taaactactt ctagtaataa gccctaccag
    22261 tttattttca tcagaaataa ccggaatata tccaaccgaa ttacgattca ttacatttag
    22321 tatttctact aaattatcac cttcatttac atgcaaaggc tcacttgaca taatatcagc
    22381 aagtaatatt gacttatcat caatatcttt catatctttt acagtaacaa taccttttaa
    22441 tgtttttgct ctatctataa ttagtaagct atcaacctta ctcgttctca taatttctat
    22501 tccttgagtt acagttctag tagaattaac agcaatagga ttttttatca taatatctct
    22561 tgcttttata tattctggat tatcccaaac tctatcttct ccgataaaat cttttacaaa
    22621 gtcatttgca ggctttctaa gtatattttc gggagtatca tattgtgcta ttcttccatc
    22681 cttcataata caaattttat cagctatctt taaagcctca tccatatcat gagttacaaa
    22741 tattatagtt tttttcaatt catcttgaag agaaaataat tgttcttgta aagatgttct
    22801 cgttattggg tctaaagcac taaaaggctc atccatcaat attatttcag catcagtagc
    22861 aatggctctt gcaacaccga ttctttgttg ctgtccacca ctaagttctg aaggatactt
    22921 attcaaaaat tcatcactat caagtcctac catctcaagt agttgcaacg ttcttttttc
    22981 aatttcttca acggattttt ccttttttag gcgtggaatt agctctatat tttcttttat
    23041 tgtaagatga gggaataggc ctgtgttctg tataacatat cctatttttc ttctaagctc
    23101 tatttcattt tcctgagaaa ttggtttgcc attaatatat atctcaccag atgtaggttt
    23161 tattaatttg tttataagct tcaaagtagt agtctttcca caaccacttg aaccaatcaa
    23221 aactaccaat gtacctgttt ctacaactag agaaatatca tctaatataa cattatttcc
    23281 tatttttttt gttacatttc ttatctctat cataatttat catccccttt ttcgaacaag
    23341 caaccttctt caatattata agttgttttt ataattatat catactattt tatttatgta
    23401 aacaaagtac gatttttagg tcactatttg tctgaatatt gctacaaata taaagtattt
    23461 atatatagta tataatacat aatgtcaaaa aacaaaaaat ataaaaatat tcataaaata
    23521 acactagcct caattatata gtattaaagc tagtgttatt tcatttaaac aaaactcaca
    23581 tttggatact atccatgtat tcaaataatg taatatcttt ataagtttct tttttatcac
    23641 ttaggtttga tattgtaact cccaatagcc taatttgttt tccattaaca tcaacatttc
    23701 tcaacaaatt atctacacca cttcttatat cattatattc gtcaatatag tgttctaaac
    23761 taaggcttct agttatcttt gtaaagtcct catatttaat ttttaatgtt aatgtttttc
    23821 caaacttctc agagtttttt aatctatgac atacttcttc acaaagttca tcaagaatgt
    23881 tgcgtgtttc ttcttcatca atatccaaat tatgacttaa tgtagtctca gccccaacag
    23941 attttctaac cctatttggt tccactggtc tatcgtctat tcctctagca aatttatata
    24001 attcataacc tctttttttg aatatatttt caagttcaaa taaatttaaa caccttaaat
    24061 catatccagt ttttatacct aaatttttaa gagtatttga agtaactttt ccaacaccaa
    24121 aaaatttatt cacaggtaat ttatctaaaa agtcctgggc attttcttcg gttattacag
    24181 ttaacccatt tggctttctt aagtctgatg caatcttagc aagaaactta ttgtatgaca
    24241 caccagctga agaagtaaga ccaacttctc taaatatatc tttttttatt tgctttgcta
    24301 tctcaattga tgaatcaata ttttttttat tttttgttac atctaagtaa gcttcatcta
    24361 aagataatgg ttcaactaaa tccgtgtatc tataaaatat atctcttatt tgctctgaca
    24421 cactcttata aacatcaaat cgaggtctta caaaaactgc atatggacac ctattgtaag
    24481 ctacagctga tggcatagct gaatggatac caaattttct agcttcataa gaacacgttg
    24541 caaccacacc tcggctttgt ggatttccac cgacaataac aggtcttcct tttaattttt
    24601 tattatctct ttgttctata gatgcataaa aagcgtccat atctatatga attatttttc
    24661 tataatgttt cattattatc accttttaaa gtataaatag attaatttta tttatattac
    24721 tttttaatta taccaaactt atgtttcttt ttggtatttt ttcattattt tctttatata
    24781 ttcatatttt ccataaataa aaccatctat aatccattaa cttcaatata atttataaaa
    24841 aatccttaaa aacacttgca aaaactatat aaaagcttta caataggtat tccaaggaac
    24901 taaatttaaa attccttatg gataacagta tatatattta atttatggga tgttttaaaa
    24961 acaaaagact gtcttatgca agacagccta ctgattttat tataattgta atggactgct
    25021 caaactatta ctttaaaaca gcccatattt ttttatataa ttttaactat agtaaagatt
    25081 ttgtgaagga tattctgggt cacaagttac atctatacca agctttctaa atgtttgttc
    25141 atcatctcta tttaatatag tagtagaatg agcttgacac ccttttaata atgctaactt
    25201 ttccatagca aattgtgcag ttggattagt tacagcacaa atactaagtg cagttaacac
    25261 ctcttggcaa ctaagtgaaa catttctact agctaaagtc tttgttttta agtttatgat
    25321 aggttctaat attacaggtg atattaagtg catatcatca gatatattgg ctaaatgttt
    25381 aatggcattt aatactactg ctgcagttcc attcattaaa tcagaaccct taccagttaa
    25441 tattgtacca tcttctaatt ctaatgcaac tacaggacaa gtatcattct tgtcagcact
    25501 ttctttcaat ttattactgt agtttctagc aggtaataca acatttctat cttctggctt
    25561 taagttaagc tcttccataa tcattttcat tctatcataa gcacctttat caacttgtcc
    25621 ctttttatac tcacaggcag ttttaaagta tcttcttatt atttcttcta tagaagcttt
    25681 tcttattact tcatcatcta ttattccaaa gccaactcta ttaacaccca tatcagttgg
    25741 agattggaaa attgattctt ttccagttat cttttctatt atttttttaa gcactggaaa
    25801 taattccaaa tctctattgt agtttactga catttcacca tacgtttcaa ggtggaatga
    25861 atctatcata ttcacatctt ttaaatcaac tgtagctgct tcataagcta tattcaaagg
    25921 atgttttaca ggtacattcc aaactgggaa tgtctcaaac ttagaatatc caactacatt
    25981 acctctttta ttttcatgat acaactgact tagacaagta ccaagttttc cactattagg
    26041 tcctggtgct gtgactacta ctattggctt tgtagtttct atatattcat ttgctccata
    26101 tccctcatca ctaactatag tctcaacatc tgacggatat ccctttgttg gataatgctt
    26161 ataaacttta attcctctac gttctaattt atttataaat acagttgtag caggttgtcc
    26221 ttcaaatcta gtaattacta cactatttac atctagttca taacctctta aatcatctat
    26281 taatcttaaa acttccatat cataagtgat accaaagtct cctcttattt tgtttctttc
    26341 tatatctcct gcatagacac aaattacaac ttcaactttt tctttcatat gttgtaatac
    26401 ttttatcttt gcattttcat caaatcctgg aagtactctt tttgcatgaa ggtctcccat
    26461 caattttcct ccaaactcta aataaagttt gtcaaaatta tttactcttt ctaagatata
    26521 tttagactgc tcttctaaat attttttgtg gtcaaatcct actttcatct taacacctca
    26581 ctaaattaaa tttcttattg ataacagtat atttatatat atttttttag taaaacatct
    26641 tataaaaaaa tgttaatact ttttcactca aagtaatatt atacaattaa gattaatttt
    26701 tttcaagata gttttatggt aaataagata tacctaatat aactaataaa ataagaatac
    26761 tatattttta tatataagta taatattctc accttagcat taatttattt caatattttc
    26821 atatatcttt tattctaaat tcaatacttt tctaatatct tctatacctt gataaacttc
    26881 ttgtttagaa aaattcatag cttctaattc ttcatcagtt ttttccataa gcatagtata
    26941 aaattctatt gctatatact gcatattttc actatttaca ctcttagctt tatcaaataa
    27001 aatttcttct gcttcgttta catttccctg aaaaactaat ctttttaaag ttataagtat
    27061 aacttcttct tctgtattat acactaaatc tttttcttca tattcactgt atttttttcc
    27121 caataaaagt cttgcaacca taagttttat ctcattgaaa atcttatcca ctatagccat
    27181 ttttacatct cctataaata aattttattt tctttttaat tatatcagac tttatcgtaa
    27241 aaattattat atgtaaatga aataattcaa aaataaattt taacaataaa aaaaagaagg
    27301 tagcggggga tttccgacta ccttcaaaca ctagattgtg attggtttgg tattttattt
    27361 atacccacca taagctttat taaatcatct aaattgattt ttttagcatc cacatcctcc
    27421 atcaaaaacg tcaccacatt cacaaagtat taataataat attaagaaag gtatccatgc
    27481 acataagtcc atttcagccc aacaatcttg gaatgctaag aataagaaga ataatattat
    27541 tatccaccaa gcacatccac caaatccgcc ttcaccaaaa aatctatcca acataatatg
    27601 ttacctccta gtcattttgt atgtggagtt tcagctccta aaatatacta tgtttaaggg
    27661 gtaatatttg ctaatgattt ttttaaattt ttttagatat ttttaaaaaa attattgatt
    27721 cacactcaat gtcttaccat cactattgat aactacctct ccattagaag tcaaaaatgt
    27781 ttttatattc aatctttcca atatttttaa aacaccttcc tctggttctt ttttctctga
    27841 acatgtaata acagcatatt ggggtgaaat agactctaaa aatgtttcag ttaatttgtc
    27901 atatcttcca tgatgaggca ttttaacaaa atcatatttt aaagtatttc catttataaa
    27961 ctctgacaat ctttcttcct ctgcatctcc tgcaaataaa aacttatgtg ctccatagct
    28021 tatattagtg attattgaat aatcatttga actctcgtac ttgctttttg aagccggatg
    28081 tatgtttatt tctgcactat tgattacaat attcatatta tcttttagta gcactggttc
    28141 tatatcagct tttttaagtg aatcaatata ttcatcatat tgcttactat ctttcttata
    28201 atttgcttgt ataatattct ttactttaac actagataac acactatcta ctccacctat
    28261 atggtcctta tctaaatgag ttaaaattaa atagtcaagt gtatttattc ctttttcttt
    28321 taacctatcc actatttgtt ttccattttt atcttctcct gtatcaatca ttatgacttt
    28381 gttacctatt tcaaccaata tacagtcaga ctttccagta cttaatatag tagtcttaaa
    28441 tgcagtattt tttgtgctac tctgaatatt attttgactc acatcatttt tgtcactttt
    28501 taaataagat tcattcgttg attttatctt actattagta gttatatttg ttacaagcaa
    28561 taatattact attatagata atgtagtaac taaaaacagg atatctttta atactatttt
    28621 cctcatccaa aagctcctat aaattttatt tattacatat ttatagtata gcaaccttaa
    28681 ctctgtaaaa tacacatata aaaagggcaa attttaggca tatttgtggc atgataaaaa
    28741 aatgactgta ccaaaacaat tatctttcta aatttacatc ttcaaattct ttaatcttat
    28801 cattagataa aacactcttt ttagtagatt gtccattttt tctatcaaaa gtatttttag
    28861 cttttacacc tgataaaaac ttacctgctc cattaataca accttcttca cacatcattc
    28921 cttctataaa attaccttct aatcttccca atttagccat tgtcattgtc tttttaattt
    28981 gtcttggacc agatactttt ataggttcaa aatcaaaact aagccccttt tcagcaatat
    29041 agttctgtac tgatttggca agtccaccag atgctccaaa tccccttcca aaaatcgaac
    29101 catcatttat atcttcattt gaacattttg ataaatctat attaaatgct tcaaataatg
    29161 ccattagttc ttcaaaagta agtacataat ctactgaatc ttttaaactt ttctctgttg
    29221 cctcactctt tttagctgta catggaccta taaatacaac tttagcatca gaatctattt
    29281 tttttactaa tcttcctgtt gctaccatag gagacactgt agatgatatt ctatcttttt
    29341 catctgggaa catcttctct atatacttaa aaaatcccgg acaacatgaa tttgtcatgt
    29401 aattatctcc tgattttatt ctttcaataa gttcattgct ttcatgaact gtaactgcat
    29461 ctgctccaca agaagcttct atcatatcta caaatcccag tgattttata gcatttttta
    29521 actgaccata tgtaatatta ctatctattt gacctgttat agctggagct actattgcat
    29581 acatattttc tttctttgca agtttttttg caacaggtgc tatcaaactc ttatcagata
    29641 ttgctccaaa tggacatgct gacatacatg ctccacaatt tatacaatta tcttcatgta
    29701 tcattgcttt catagtgttt ctattaaaat ccaaagctcc tgttggacaa acgcttttac
    29761 aaggtcttac aacttctgat atagcatcat attgacatga acttttgcac attccacaag
    29821 ccttacataa atcatgattt atatatgctc tcccatttat ataagaaatt gccccaaatt
    29881 tacatgcttc cttacatcta tgagccaaac accctctaca aagctctgta actgtaaatt
    29941 catttattgg acatctatca catgctgctt caactatata tataatttgc tcatctgtgt
    30001 ccaaactaac tgaatctgga tttatatctc catatttact atttggagtt aaacttaatg
    30061 caagttgcgc cctttcaaga actacttctc tctctttata aacacaacat ctatatctcg
    30121 ccttttctcc tttaattatt tcatatggaa ttttttctat attttctttt gttaaatttt
    30181 cttcaataga tagtcttgct actgctgtta aaacttcatg ttttaacctt tttagttgcg
    30241 tttcaaattc aaacataagt aatcctccct ataaatcaca cctttttgct atatttctac
    30301 tattatgtat attgtatttt ttatttattt ctataaatta ttataacata ggcatatact
    30361 ttgttaagtt atatacaaaa tattatatat gtaattttta ttttaagtgt atataatttt
    30421 tattttagtt gttcaataat taaatatata aataaaaata gagttgtcca aaagttaaac
    30481 ctcaacttag aacaactcta ttaacaatat gtatttttgt tgctttgtat tatctttatt
    30541 tttctaaatt tatattacta aaatctttta tctttgtatt tgctaagaca cttttcttag
    30601 ttgattgaga attttttcta tcaaaagtgg ctttaacctt tgcacctgat aagaatttac
    30661 cagaaccatt tatacaacca ccttcacaca tcattccttc tataaaatta ccttctagct
    30721 ttcctaaatt agccatagtc atagttttct tgatttctct tgaaccagaa atctttacag
    30781 gttcaaaatt aacaccaata cctttttcag caagataatt ttctatagct tttgtaagac
    30841 caccagcagc tccaaaacct cttccaaaaa ttgatgcatc atctatatct tcatttgaac
    30901 acttagccaa gtctatatca aatgcctcaa ataatgccat tatttcttca aaagtaagta
    30961 cataatcaac agctcctttt aattctttct tagctgcctc actctttttg gctgtacatg
    31021 gtcctatgaa tacaacttta gcatctggtt catgactttt tataaatcta ccactagcta
    31081 tcataggaga cactgtagaa gatatttttc ctacatgatt tgggtatgtt ttttctatat
    31141 aaccaacaaa tcctggacaa caagaatttg tcataaaagc ttctcccttt tccattcttt
    31201 cagcaaattc taagctttca tgaactgtaa ctgcatctgc tccacaagct gcttctaata
    31261 catcaacaaa tcctagtgat tttatagcat tttttatttg accatatgta acatttgctt
    31321 caacttgacc tgttatagct ggagctacca ccgcatacat tttctctttt tgaactaact
    31381 ttctagctac aggagctatc aaactcttat cagatattgc tccaaatgga catgctgaca
    31441 tacatgctcc acagtttaaa catttatcct catgtatcat agctttcata ttttctctat
    31501 caaatcctaa ggcattagtt ggacaaacac tcttacatgg tctaacaacc tctgagatgg
    31561 catcatattg acaagctttt ttacacattc cacaagcctt acataattca tgatttatat
    31621 aggccattcc tccgacatgt gttatagctc caaatttaca agcttcttta catctatgtg
    31681 ctagacatcc tctacaaata tctgttactg taaattcatt tattggacaa ctgtcacatg
    31741 cagcttccaa aatatatata atttgttcat cttcttctat atccattgta tctaaatcta
    31801 tcttatcttg acctggttgt aaccccatac ttacatttac tctctcagat attatagctc
    31861 tttctttata aacacagcat ctgtatgtag gtttctcacc ttttattatt tcatactgaa
    31921 tattatccat tgcttctctg gtaaggttat cttcttttcc aagtctagct attgcagtta
    31981 aaacatcatg tcttaatttg tgtatttgtg tttcaaattg aaacatttta ataccacccc
    32041 cgtaaatgat tttatacgat accattataa cacaatatgt ccttttatta atatatacat
    32101 aaaatattat atgtttaaat tttattttat cgaatatata tagtgttttt ttgttttatt
    32161 atataatatt attgatattt ctgaaatttc actattttta tttttacttt tcaatctaat
    32221 atataatatt ttttattata tattttaatt ttacttttta cttacatacc tttttgttac
    32281 attttttgat attaaattta ttatctacat ttacattttc gtcaaatttt acaatacttc
    32341 tcactgtata atttaacaat ttttattaaa ttctaaaagt aattaatttt tttaaaaatt
    32401 ttatttgctt ttatttaaaa tttatgttat attaatagaa cagacttatt tttttagttt
    32461 gtatttttta ataatgcgga ggttttctta taatgaaaaa cggaatagta aaatggttta
    32521 acaatgaaaa aggatttgga ttcatatctg tagagggaga agatgatgtt ttcgtacatt
    32581 tctcagctat acaaaatgat ggatacaaaa ctttagaaga aggcgaaaag gttagctttg
    32641 atataactca aggtaataga ggacctcaag ctgaaaacgt aaatagaata taattattta
    32701 atttaaatat atatatttta gaaaaggagc tatcgagggc tcttttttgt tgtttattat
    32761 atttattttt aattacaaaa ataagaagtt actttagaaa agcgtcaaac ttaaaataac
    32821 ttcttatatt ataatatata ctatttatat aaaatactct atttaatttt atcagatatt
    32881 attaagcata actctcttaa gattttacaa gctataactg tcgatacacc tgttgtatcg
    32941 taatcagggc ttaactctac aatgtcacaa ccaactatat ttatattaga gttttttata
    33001 atcttaaaaa tctcttgaaa ctctctataa tttacaccac caggttctgg tgtacctgtt
    33061 cctggaaaca cagatgcatc caatacatct aaatctatag ttagataaat attctttcca
    33121 tttagcatat taactatatt ttcaaacgta tctattcctc ctatttccat gtatgtgtgt
    33181 ttttcttctg tagcaaattt aaattcttct tttgtcccag accttatacc aaattgaaat
    33241 attttattat cacctacaat atcccatatt cttttaatta ctgttgcatg agaattttta
    33301 ctattattat attcttccct caaatcagta tgggcatcaa aatgaattac atatatatca
    33361 ttatactttt catgtactgc tttaaaggct ggtaatgtaa ctaaatgctc tcctccaatc
    33421 ataaagggta cctttgaatc tctaacaatc ttatatgtct cttgatagat ttcttttaag
    33481 acttgttctg tacttccaac actaatttct aaatctccat aatcacatat attataatcc
    33541 tctaaatcca aatctaaaaa aggactatat gtctcaagac cgtaaaattc ttttctcata
    33601 gagctacttg caaatctagc cccaggtctg ttagaagtag ttccatcaaa tccaacacca
    33661 aatactataa gattagattc ttcataattt ttgttcatac tcataaaagt gttcatatga
    33721 tagaaattat tcttcatcca gcatctcctt cacgtattga ggtaacataa acgaacctaa
    33781 atgtatatca ctattataat atttagtttt taaacttaat ttctcccatc catttctatc
    33841 ttgatttttt actggattaa gtttttttga agcaaatcca aacaaccagt gaccagatgg
    33901 atatgttggt atatgtgctt gataaacttc tgatattgga aatatctgtt ttaacttttt
    33961 attagctctt ttcatctctt tagcattaaa gtcaaaatac ggactttcac tttgatttac
    34021 taaaattcca tcatcagtca gtattctata acaatcctta taaaaatcag ttgagaaaag
    34081 cccttcacct ggtcctattg ggtcagttga atctactatt atcaggtcat atgatttatc
    34141 atgtgcacac ttaacaaaat taacaccatc ttcaaaatat aaactcactc ttttatcatc
    34201 taatttacat gagcatatat ccatatactc tttagataca tctacaacca ttttatctat
    34261 ttcaaccatg tcaatctttt ctatttgagg ataacggctt aattctctta ctgtacctcc
    34321 atcacctcca cctatgacta atactttctt tatgtttaaa tttgtagcca taggaacatg
    34381 agttatcatc tcatggtata taaattcatc tttatatgtt accatcatta atccatctat
    34441 tgttagaaat tttccaaatt catcactatc aaacacatct atcctttgaa attgagactt
    34501 tccttcaaat agatgtttat ttacttttat agaaaatctt acattttctg tccattcttc
    34561 tgtataccaa agttccatat aataatcctc actttctctt gtatgtttta tactgcttct
    34621 ggtttatgag taatcttacc taaatctttt tttgaatatt ttttaatttt tgtagttaag
    34681 cctcttggaa tttctgtaga ctcgcttctt tcagacttta aaagttgctc taataattca
    34741 aaacttttcc atgggtctat atcaccacaa gtaaaaaaat ctgctgctgc ataaccatat
    34801 tcaggccatg tatgtattgt caaatgagat tcagcaatta taacagctcc tgaaaccccc
    34861 caaggattaa aatggtggaa tacagaatcc acaattgtcg cttttgcatt taatgctgaa
    34921 tcattcataa aattttctat aagacgtggg tctttaagta cgttttcatc acaattatag
    34981 tattctacta gtatatgtct tcctagtgtt tcaaatttca tttcaacccc aactccttta
    35041 tacttttaat aagttctatt tgataaactc tacttaacaa acacttgccc ttgcaaaata
    35101 aagtaattta ttttattact agtattctat ctaagttttt atcttgtaca tctgtaagat
    35161 atgcattgct attttttaag aaaataatat actccataat ttcttctgtt atcaattctc
    35221 ctggagcaat aatcggtata cctggtggat atgccattat tgattctcca caaattctat
    35281 ctatacatga attgatttct acactttcct ttggtgcata aaaagcttct cttggattaa
    35341 gtttaataat tggatttatt tgaaccatat taaattcatt taatgttgat tcttttctat
    35401 aaatctttgc aattatagaa agtgccttta ctaatctatc tatatctgat ttatttgttc
    35461 caatggacac aagtgccagt atattatatg aatctcccaa ttctacttga attgaaaaat
    35521 ttttatataa taaatcgtat acttcaaatc cagtcaaatt taattctttt acatttatac
    35581 acaattttgt ttcatctata aatttaacac ctttttgttt caatatttct gtagataaaa
    35641 cttttattcc atcaattttg ttcaatttag attttgcata cctagataga tttaatgctt
    35701 ttgacaattg cttttcacca ttctcaacta gattaactct agcaccatct atacttgcca
    35761 taagcaaata agatgctgat gttgattgta gcatattgat tgattgaaga accttttcaa
    35821 aacttacatt atctctattc aataagagtg ctgaagattg tgtcaatgct cctccagttt
    35881 tatgtatact tacagctacc atgtcagctc ccaaacttat tgcagaagga ggtaaatcta
    35941 aatgaaatgg aaaatgtgca ccatgagctt catcaactaa cactaaaaca ttatttttat
    36001 ggcatagttc tataatactc tcaaggtctg cacaaactcc atagtaagtt ggattaagga
    36061 aaaataatgc ttttatatta caatcttttt ctatctctgt tctaactttt tcaagagtaa
    36121 tatttaaact tatacctagt ttttcatcaa attctggttg tataaatatt ggtattcctc
    36181 cacaaagtat taacgcattt attactgatt tatgtatgtt tcttggaagt aatacttttt
    36241 cacctggttt tataacactc aaaatcattg catgtattgc ttgagttgtg ccattcgtta
    36301 taaagaatgc ctcatcagcc atatatgctt gtgctaacaa atcttgtgca ttttttataa
    36361 tcccatttgg cgcagataca ttatccatag ttggtgatga atttatatcc attttcatga
    36421 gactttcacc tagatattta tttaatatag ctacacctct attttttaca tgtccaggca
    36481 catcaaaaca agctatatct ttctctgagt attcctttaa cgactctaat aatggagcac
    36541 actcatagct taattcaact gcatttgcaa actcgctatc catgatttca aattcatctc
    36601 ctctctatat tcaaatattt ttaatattta ttatttatta tttctatttt ttatttttac
    36661 ttttattaaa ctcatgatat ttattatatt aacatcccag catttttaca agtgtttttt
    36721 tgaagttact caaaaacata tacattagct atatcaatat ttataaactt tttaattttt
    36781 tgttgtttat attaattttt ttaaaactta gtatttataa actttcttat tttttattat
    36841 tttggttttt ttactttttt gagttttagt aagcttttga gtttactatt tttaatttat
    36901 aagtatttta ttactaaact tttatattat tcttatttca catatcaaaa ttcttggttt
    36961 agttggacta aataattttc ctatttttgt attttaattt taatataaaa tttattaaat
    37021 tttttaattg ataaaataaa ttctatattt atgatattaa aattctataa gttcattgat
    37081 ttatatatgt taatttaaat acattattct atcgtagtgc cttagagttt ttatttgtat
    37141 ttaaagttaa atttattctt gtttttcctt taatatttaa tagtaaaaca acaaaattat
    37201 aaaaaaaata aagagaaact ttataaaagc ttctcttttt tcatctctgc tataggtatc
    37261 gttttccaat gttctctcaa aattctttca acattttcta aatcattctc cctcatagat
    37321 tttactactt caatatgttg attattgctg tatgtcaaca ttttatataa ctcatcttca
    37381 ttatcactag tatatgactt tgacataaaa ttatatctta aagagttcaa tagatttact
    37441 aacacctgat tatctgattt agttatgtat atatcgtgaa attcattact taattttaaa
    37501 tagctattgt aatttttata ctttatagag accgccatca tttctgttaa ttcttccatt
    37561 tttactaggt ctgattcagt taaattttgt aatgctgaag aaccggctaa cgcatccaat
    37621 actccaatta cggaataggt gtctaatttc tccttttcct gaaattcttt tactacaaat
    37681 cccttattag gtattttttc tagtaaattt tcacctgcta acctagttaa tgcttctctc
    37741 gctggtgttc tactaactcc gatagattta caaatctcag attcagtaat tctctgatta
    37801 gcttcatatt ctccattttg gattttcttt gatatatagt catatacttg agttgtaagt
    37861 ggatctacct tcataagacg ttcctccata ttaagattat aaaagttaaa tctccctaca
    37921 actttggtag agagttcagt tttatcttac taatttatat aatatcacaa ttatgatatt
    37981 ttttatactt ctttgtaata ttttgaaata ctaaaattct tatgataatt tatacaaaaa
    38041 aagtagaaaa agttttgata aaacaaaact ctttccacat tcgccttatt ttctatttat
    38101 ttttctaaaa aaagatattt aatttactct aaaaattcta aatgatttag agtctaaatc
    38161 tactttcaac tcattatttt caatttgtat agtactattt gcatttttaa gactatactt
    38221 tataggtatt tcctcaataa aactactctc tatacataat gatatacttt ccacattttc
    38281 acttctattt actaaaatta acattctatc attattttca ttaactctaa taaaagaaaa
    38341 tacagaatca ttattagtat atataaattc tgtatttcca gaacttaaaa gtttattctt
    38401 atttctaatt tttataacat ctctataaaa attaagcata tcttcgtctt catttttcca
    38461 tggataagtt cttctattat ctggatcaac acctccacaa agacctgctt catctccata
    38521 atatatatat ggtactcctt caaatgtcat ctgtatagct acagcaagct tcactaaatc
    38581 tacatcatca tttaattccg ttttagctct tgtaacatca tgtgttccaa ttaaatttaa
    38641 attagaccta aatgattctt ttggataatt ttctttaatc tgcatatatc tattacaaag
    38701 ttcatatgaa ttaatttctc cttttaagaa agaaaacatg ttatttctaa aaggataacc
    38761 cataacagaa tctaattctt cacctaataa ataacttctt ctctgaccat agctaatctt
    38821 atttgatgca tcttcccata cttcacctat aagaattgag tcatcatctg ccttttttaa
    38881 ttcctttttt aactctctta taaacttagt tggtaattcg tctgcaacat ctagtctcca
    38941 gcccttaata cccatattta cccatttatt tataacgcta tcattgtcat atattatata
    39001 atccatatag ctatgttcta attcatttat atttggcaat gtcttaacat cccaccaact
    39061 tttatatttt tgaggaaatt cttcaaacat ataccatgaa taatatggag attctttaga
    39121 ttgatatgct cctaaactat tatagtttcc atacatatta aaatatttac tatctgctcc
    39181 agtatgacta aaaactccat ctaatactat tgatatacct ttttcttttg ctttatcaat
    39241 aagttcttta aatgtatctt catctccaaa cattgggtca atttttttat agtcacctgt
    39301 atcatactta tgattactag aggcttcaaa tatagggctt agatatagta tacttacacc
    39361 taattttttt aaatatccaa gtttattgat tatccctctt aaatttccac catggaaatc
    39421 ccatcttata acatcccctt gactatcctt tatgtacata ggtgtatctt cccaatttcc
    39481 atatatgaaa ctattttttt taggattatc aaccttacca ctacgatttc cattattgaa
    39541 tctatctaca aatatatgat aaagtattcc ttctttgtac catgttggta ctttaaaatc
    39601 tttataaaca gtgatttgat atttattaat atcactataa ttatattcac aagaatatcc
    39661 attatctctg ttttttccat aaaataaagt ctttatttga ccatccattt caacatcaac
    39721 tttaaagtaa taaaaataaa cacctgtacc atcaaacttt tcaatcttac cacaaaagca
    39781 ctttccaagt ttatcattat ctaattcttc atttaaagtt attgttctca tttcttcttc
    39841 tctatttatt tctagggaaa tagacttgat attgtatccc tcatttactt taacacaaat
    39901 ggtcaattcc tcatcaaatt ttaaagctcc aaacggagcc ttaaaatttt tatcccaaga
    39961 gttatattct attacacttt gcatttaatc acaccctttt aaaccaaatg tcttttgcat
    40021 actcagaaat tgttctatca gaagaaaaaa atccagagtt agctatatta actaaagaca
    40081 ttttattcca tttttcttta tctctatata acctgtttat atctgcttga gcttgaccat
    40141 aattttcaaa atctctcaat acaaaatact catcattgta agtagtgagt gaattataaa
    40201 tacttcttcc ttcttcgcca agattaggaa taaatccatt tattaaatca tctactaccc
    40261 tttttatatc catattagaa tgatataggt caagtgaaga atatccacca tatttattat
    40321 aatttagtac ttgttctgca ctaagtccaa ataaaaacat attttcttta ccaacctgtt
    40381 cacatatctc tacatttgca ccgtcaagtg tagccaaagt tatagcacca ttcatcataa
    40441 acttcatatt acttgttcca gatgcttcct tagttgtagt tgaaatttgc tcactaacat
    40501 ttgctgctgg tattattatt tctgctaatg atactccata attctctaaa aatactactt
    40561 ttaacttgtc ctttactcta acatcattat ttatagtact tgcaacagaa tttataaatt
    40621 taattataca tttagccaaa taatatcctg gtgctgcttt tgcgccaaat ataaaagttc
    40681 ttgggtcaag attcaaatta ggattatcaa gtaattcatg atacatatga agtacattaa
    40741 atatattcag taactgtctt ttatacgcat gcaatctctt aacttgaaca tcaaatatcg
    40801 aacttgggtc tacatttaaa tcgtatttat cttttataaa ctttgctaga tttgctttat
    40861 tattgtattt tatatcatca agtctttgta aaacagagga atcattttta aatttttcaa
    40921 tattttttaa ttgtaatgta tctttcttcc atgaatcacc aattaaatct gtaattaaat
    40981 tgctaagttg aggatttgaa cttataagcc atcgcctatg tgcaattcca ttagtcttat
    41041 tattaaattt atttggttca tcttgataga aatcttttaa tacctctgtt tcaagaatat
    41101 gagtatgaag cttagcaact ccatttacac tatgacttgt gacaatacat aagtttgcca
    41161 tattgatatt gtcacagtct attatactca ttctttttat tctatcttgg tcatatcctt
    41221 tattatttaa ttcctctaca tatcttctat ttatttcttc tattatcata taaattcttg
    41281 gtaatagctc tttcatcata cttacaggcc atttttccat tgcttctgac attatagtat
    41341 gattagtata ggacactgtt tttttagtta tttcccatgc ttcatcccaa gataaatttt
    41401 cttcatcaag caaaattctc ataagttctg gtatgcataa ggtaggatgt gtatcattta
    41461 tatgaattgc taccttatcg aataaattat taatatttaa tttgttcttt ttatattttc
    41521 ttattatatc ttgtatccct gcacttacaa aaaagtattc ttgtttcaac ctcaacaact
    41581 tacctgcata gtttgaatca tcaggatata atacctgtga tatttcctca gtataatact
    41641 tatgttttaa agcctcttca tagctaccag aataatttag agcatttgag gtaagttcac
    41701 caaaatctct ctttggtatt tcactcttaa acaatcttaa agtatttata cattggtttt
    41761 ggtatccgat tattggtata tcataaggca ttgccattac aggaaggtag tttttatgaa
    41821 ttacttttaa atgactacct tccttgatta attctacctc accaccaaac ttcaccattg
    41881 ttgcttcatt tggtcttaca gtttcccatg catatcgtcc ttctgtaagc cagttatcag
    41941 gcacttctac ctgataacca tttacaaatt tttgttcaaa cagaccatat ttatacctta
    42001 ttccatatcc ttgaccactt atgttcaaag atgccataga atctaaaaag caagctgcta
    42061 atctaccaag tccaccattt cctaaggctg ggtctacttc agtttcaatt aaatcatcca
    42121 agttgattcc taattcagat aaaccttctc taacttcttc ttcaatattt aaatttaata
    42181 aattagattt taactgtctc cctattaaaa attctaaact aaaataataa acttcttttt
    42241 tcttatctaa ctttgtagca acccattttt ttgctatctc atctttcaat agactacata
    42301 atacatttaa caattgttca tcagtagctt ccttgataga ttgtgcatat aagctataca
    42361 tttttacttc aaatttttgc ttgaatttct ttttgcttat tgttaccaaa aaaaccactc
    42421 ctttaatcac atatgtctct atatgtttct atatactcct tagctgattt tttccaacta
    42481 ttatctgttt tcattgcatt ttcaactaat ttaatccatt tttctttgtc cttaaatact
    42541 ttgattgcat ttttcaaaca gaaaaacatc tcatgagcat tgtaattttt aaaactaaat
    42601 ccattgcctt ctccagtaaa tttattataa ggtgttactg tatctctaag ccctccagtc
    42661 tctctaacta ttggtaaaga accatatctc atagctaaca tttgacctat accacaaggc
    42721 tcaaataatg atggcattaa gaacatatca cttgcagcat atatctgttg agccaatgat
    42781 gcattaaaag taattctagc tgatacttta tctgaaaaat tagaatcgta atagttgaac
    42841 attgattgat attgatgttc tccagttcct aaaacgacaa tctgtaaatc ttctctcata
    42901 agttctggca tcatgtatgc tattaaatca aatccttttt gagaaactaa tctcgaaact
    42961 atacctatca acggaataga tgaatctttt ttaagtccca atatatcttg taatctcaat
    43021 ttattttcta cttttttgtc aattgagtct acatcataat gaacaaatat atctttatct
    43081 tttgcagggt catttaaatc ataatctata ccatttaata taccttttaa ttttcctgac
    43141 tcttttctaa gtaatccatc taattgttca ccataaaaag atgtttgtat ttcatttgca
    43201 taagtaggac taacagttat aattttatct gcaaaattta taccagcttt cataaaattt
    43261 attgagccat aatactctat atctccatta tcaaagtatt tttgtggtaa gtccaatata
    43321 tcatataaaa catgcttatc aaatacacct tgatattgga gattatgtat agtatacata
    43381 gtttttatat ttttataccc ctcaagggtt gcatatcttt cttttagaat taatggtaac
    43441 atccctgtat gccaatcatt tatatgtatt acatctggat aaaaatcaat tctacttata
    43501 gcttctaaaa ctgcatctgt aaagaaagta tatctttctg catcatctcc atatccatag
    43561 atactctttc tttcatcttc tcttctaaaa taaaattcat tatctataaa gtaaaatttt
    43621 acaccatcta attccatttc taaaagacca cagtattgat ttctccatcc aacacgtaca
    43681 ctaaacacag ctatttcttt tagttgatct tttaaataac ttggtatagt tgaatatttg
    43741 ggcattatta cccttacatc tacaccttct ttttttaatt ctttaggcaa tgcataagaa
    43801 acatctccta atcctccagt ttttgcaaag ggccaacact ctgctgttac ataaaaaacc
    43861 ttcattagtc attccccctt ctaaaaaatt tatttatata tttgtattct tttccactac
    43921 aagaggatat ttttcatctc ctcttaattc tttcccattt gatacactcg tatttttatc
    43981 taatattgca ttatatagtt ttgcatttga ctttattaca caattttgca ttactattga
    44041 gttctctata actgttcctt tttcaacatt tacctttcta aatatgatac tatcttttac
    44101 agtaccttct attaaacaac cagatgctat aaagctattt tctactttag cactatctgt
    44161 ataaattgta ggtgattcat tcttttcttt tgtaaatatc tttctttcag aatataataa
    44221 ttcatctgca acttctaaat ttaataaatc tttgctcatt tcaaaatatg attcaacaga
    44281 atttacacat tttagatatc ctgtatattc ataggctcct attttcatat tttctgcttc
    44341 ttcaataata aattcttcta aataacaata attgccttta tttacaatgt tatatataca
    44401 ttcaacaaaa tcttcacgct tcataatata tgtctccata catatgtttg ctctagggaa
    44461 cttacctaaa ttatttccta catttattac cctattgtta ctatctaaat ttagagttaa
    44521 gttaccagca aaatctttat ttgcattatt tacattttta tatattatag ttatatcatt
    44581 atgagatttt ttatggtatt ccaatgcttg agaataattt aaattgcata gcatatagct
    44641 tggagctatt aaaatatatt cttcttcact cttttctata taatctagat tagctaagat
    44701 agtatatata tcaccttctc tatgtgagta agtatttcta tacttagtgt tttctggact
    44761 aaaaattgat aggccacctt tttttctact taaatcccaa tctttaccac tacctatatg
    44821 gtctgttaat gatctgtatt tttcttttgc gaatattcca actttagtga ttcctgaatt
    44881 tatcatatta gataaagcaa agtctattat tctatatctt ccagctattg gtgtagatgc
    44941 tattggtctt ccatagttaa gtttgtttat tgctgggtct ccttttttgt ttaaatttat
    45001 tatccctaaa cattcatttc tcatcttaaa gacctccttc tattaatacg catctaggtt
    45061 cgatattttc atactcagaa actacattta tttcattatt tacattcttt atctgaacat
    45121 tatcatttat agttgcacac tcacctatca tagctttttc tatagttaca ttttcaccta
    45181 ttacaacatt tggcattatt actgaatcaa ttactttact tcctttacca acaacaactc
    45241 catgtgataa tacagagtta cctacttcac ctaaaattct acaaccatca gctatcattg
    45301 atctatgaac atttgcattt ttacctatat attgtggagg cattgccata gtatttgtat
    45361 atatcttcca cttagggtct gctaaatcaa gtgtttctgg agattttatt atatccatat
    45421 tagcatccca tagactttgt atagttccta catctctcca gtatccttta aatggataag
    45481 catacattcc aacattatct tctaacattt taggtattaa attttttcca aaatcatcat
    45541 aatttatttc tggatttttt tctgcttcct taaaataatt tctcaacatt ttccaatcaa
    45601 atatgtatac tcccatagat gccaaattgc tcttaggttc acttggtttt tcttcaaatt
    45661 catagatact tgaatcttca tttgtattca ttatcccaaa tcttgaagct tcatcccaag
    45721 gaacttcaat cactgctatt gtagctttag agcctttttc cttatgaaat tttaacattt
    45781 tgctataatc catcttgtat atatggtcac cagataatat caatacatat tctggattat
    45841 aggtatctac aaagtccata ttttgatata tcgcatgtgc tgtaccatta taccaatttc
    45901 cttctttttc attcataaat ggttgtaaaa catagacacc accatttatt ctgtccaaat
    45961 cccaatgact tcccatgcct atatgtgaat ttaatatcaa cggtctatat tgagttaaaa
    46021 cacctacagt atcaatacca gagtttgaac aattgcttaa cacaaaatct ataattctat
    46081 acttacctcc aaatgataca gctggtttgg caattctttt tgtaaaaaca cctaatcttg
    46141 aaccttgccc tcctgccaaa atcatagcta acatctcttt tttcatttaa tgacactccc
    46201 ctcatctagt aattaatatt tataattgca tttttacttt tataaacttt atcagtcaat
    46261 aaatgtcgtg tataaatgtt gcttaaataa taccttaata aaaataaaat aatatcaact
    46321 atacaattga tataatgtat accccataat ggacttaccg acgtatacat tattttccat
    46381 aagcactata gtatttatta atattcaatt attggttaaa aaataataca aattttattt
    46441 tataaatata aaaacatttt ttataaatcc tttgtgtcat atgaacatgc ttatttttta
    46501 atttgtt
//
LOCUS       DAEPIN010000025        45568 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.025, whole genome shotgun sequence.
ACCESSION   DAEPIN010000025 DAEPIN010000000
VERSION     DAEPIN010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 45568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45568
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.025"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            <1..146
                     /locus_tag="KR250_001615"
     CDS             <1..146
                     /locus_tag="KR250_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HBH0067554.1"
                     /translation="EEYFKYTDKSIEVNPLRLKDKVKVENNFDADLEDNRINYISNIG
                     MII"
     gene            147..590
                     /locus_tag="KR250_001616"
     CDS             147..590
                     /locus_tag="KR250_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0067555.1"
                     /translation="MKVDKTINFLNGFKKTKPCNKIYKIIIVLILTCILFKSFMYVKS
                     INELEDYISQNKLVDKSRHTLPTTDKTREKFSKIKLNEIKKIYETIGINIITKISIDK
                     NKLEIEGVCSDLDVLNNLRKIKYIKYYYIDSIVRDGEYYRFRIKS"
     gene            605..1189
                     /locus_tag="KR250_001617"
     CDS             605..1189
                     /locus_tag="KR250_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0067556.1"
                     /translation="MRLNNREKNLLKILLILVIILASDIAVSYLEENLTSLKNEKQNI
                     INKINNQDNYQIKDNKHIDDKYIESVESNKNEELKNNFNNSFLDKKTNKDIVLLVENE
                     LRDIVNVEYINQESSLDNHNKKQVNLEIKVNGELANLLKLEGVMKKLRLNNKLEKIEI
                     NKLKVNNFDVQNIPNEYKQEKDNIVECKMTVRVI"
     gene            1442..2479
                     /locus_tag="KR250_001618"
     CDS             1442..2479
                     /locus_tag="KR250_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C domain-containing protein"
                     /protein_id="HBH0067557.1"
                     /translation="MLGRNELCPCGSGKKYKKCCLNKDIVSERACRKTELSQKQYTDL
                     YSKLYDYSRQDKFKEECEKAKEMFYIMQNDSVNEKFERFFNTYLIQDHIMENKKVMTV
                     GFLEENGANLSQSEVSILKSMFESYVSIYEVKEVSAGKIVLKDCLSGNELCTEDVKLL
                     RSFKIGSCMIARIVDIEGTNILIDITISISNEVKDIILKDIMNLFNQYQDVYKDMKSF
                     LIYHTHILYKYIQQLLDPSIAEYLKRERDKNDTKVNSENELAEEECKVLDTLKENVEK
                     ENIDACIEFWNEYKKNHENIKGAENGWAAAVEYYVKKEAGQPITQVQISKKYDISSST
                     LGKRYKDLKIS"
     gene            2560..2925
                     /locus_tag="KR250_001619"
     CDS             2560..2925
                     /locus_tag="KR250_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0067558.1"
                     /translation="MSLSLGNAIAVSTNFIKSNPAVPVYNGEEIYAVIYHIEIGYNNG
                     VSPDTKQYIIIDYKEFDLSMDKKNELINIAKEYCKSKGIVENENLEINLLDNEEAENF
                     LEKVFNNMKVIRGLITKKG"
     gene            2930..3247
                     /locus_tag="KR250_001620"
     CDS             2930..3247
                     /locus_tag="KR250_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0067559.1"
                     /translation="MDYIGIENITPYENTYEFSVYEYDDEITLGSEKLYVCELRVVLI
                     KVNSLYVERLHKSVEAMVLVKNLKKDLDKTLVVNKIKNFVLDEIWVENLVKENIEVIF
                     VES"
     gene            3295..4899
                     /locus_tag="KR250_001621"
     CDS             3295..4899
                     /locus_tag="KR250_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0067560.1"
                     /translation="MLRVSNVKLGIDKDISLLKDVILKKLKIKEKNLVKYSIYKESID
                     ARKKGKMEFVYSVDVEVKDENKILNSKIKDVTKIKEIRYVNVPVGDKKLKNNPLVIGS
                     GPAGLFAGLLLAQMGYEPIILERGLDVDSRTKDISDFWTTRKLKNNSNVQFGEGGAGT
                     FSDGKLTTRIKDIRCRKVLEELVQFGAPDEILYSHKPHVGTDILKNVVKNIRNEIISL
                     GGQVRFNSKVTDIVVSNDSIQSVIINDTEKIDTDTIILAVGHSARDTYEMLYSRGIKI
                     VQKPFAIGARVEHPQDLINKAQYKDFYNHPRLGAADYRLIEHVSNGRTAYTFCMCPGG
                     SVIASASNDFEIVTNGMSEHARDKINANSAFLVNVTPEDFKSDNPLAGVHFQQKYEKL
                     AFELGGKNYNAPVQLVGDFINNKTSLNLGSVEPSYRPGYTLTNLSECLPSFVTETMRE
                     AFISLDKKLNGFAMYDAVLTGVETRSSSPIRIVRDENTLESVSIKNLYPCGEGAGYAG
                     GIVTAAVDGIKCAEKIIQKYTNNTKI"
     gene            5023..6651
                     /locus_tag="KR250_001622"
     CDS             5023..6651
                     /locus_tag="KR250_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HBH0067561.1"
                     /translation="MNIIISLMGGLGLFLYGMNLMGEGLQKSAGTKLKKIIKLLTSNL
                     FMGVLVGTGVTAVIQSSSATTVMVVGFVNAGIMTLKQAIGVIMGANIGTTVTAQLVSF
                     DLTGMAPVALGVGIILYLFGNKPRIKNIAEILIGFGILFTGMDFMKMAVEPLRDYQGF
                     TDLLVTFGRYPLLGLLLGFGITAIIQSSSASMGMLVALAAEGLVPLSAALPILYGQNI
                     GTCVTSLLSSIGANKNARRAAMMHLIFNVLGTVIFLIFLNKPVVSMVTSWDPSNVARQ
                     IANTHTLFNIISVLILLPFTSLIIKLAIKLVPDRAGDIDEDETKTIKYIDDRMIETPS
                     IALANTIKEALRMGEKAKESLNASMEALVEHSTEKIDKTYRRERLINDLQKAILNYLL
                     KLSKAALDDDSREVVDTLFNTVNDIERIGDHAKNIAELSQVAIDSNIYFSEEGQSELD
                     VMYNRVVSAYTYALESMRSDNVDLACKVIKIEEQVDIMEKSCRANHMYRLNNNLCSIE
                     NGVIYLDVISNLERISDHAVNIAQQVIAKRLGND"
     gene            6916..8265
                     /locus_tag="KR250_001623"
     CDS             6916..8265
                     /locus_tag="KR250_001623"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HBH0067562.1"
                     /translation="MGKINFDYSKATEFFCQNEIDVMQPYVDVAHDMLHNKTGLGNTF
                     LGWIDLPKNYDKEEFDRIKKSADKIKSDSDVLLVIGIGGSYLGSRAAIDMVSHSFRNG
                     LKKEQRKAPEVYFVGHNISSTYIMDLLDIIEGKDISVNVISKSGTTTEPALAFRIFKD
                     YLEKKYGKEEARKRIYATTDASKGALRQLATEEGYETFVIPDDVGGRFSVLTAVGLLP
                     IAAAGLDIDAMMKGANDAREAFQNPDLKSNDSYRYAVARTILHRKGKDVELLVNYEPQ
                     LHYVSEWWKQLYGESEGKENKGLFPASVDFSTDLHSMGQYIQDGKRLLFETVLNVENC
                     KRNITISSEEVDLDGLNYLAGKTVDFVNHKAFEGTLLAHTDGKVPNLVINIPQLDEYN
                     FGYLVYFFEKACGISGYLLGVNPFDQPGVEAYKKNMFALLGKPGYEKEKEELEKRLK"
     gene            8421..9500
                     /locus_tag="KR250_001624"
     CDS             8421..9500
                     /locus_tag="KR250_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HBH0067563.1"
                     /translation="MSRRKKGISLVILVAIISSILSSFLTIILVKDNLVSKSTGSSTP
                     IVVNDDGKSQNIYQAVAEKATPSVVGITTTSVDTSNMFAIPTETQGVGTGIIVDSNGY
                     ILTNSHVISDGQATSVNVLFNDGSTTSGKVVWFDQQLDLAIVKVDKTGLTPAEFADSD
                     KVKVGDISIAIGNPLGLDFQKTVTQGIISGLDRTIQTEKTNMTGLLQTDASINAGNSG
                     GPLLNQKGQVIGINTAKASQAEGLGFAIPINTAKSIVEEVIKNGKYEKVTLGIKGTDV
                     SNYEAATGTKLSTDKGVYVAEVISGSSAEKAGVKVGDIITKVGDTDITGMNDLNKKLY
                     TFSKGASTKITVNRGGKAVTINVNF"
     gene            9845..10753
                     /locus_tag="KR250_001625"
     CDS             9845..10753
                     /locus_tag="KR250_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HBH0067564.1"
                     /translation="MNPLVINLQKCSIHDGPGIRSTVFFKGCPLECVWCHNPESQTYT
                     KQVLYNEERCSKCEACINICPHKAIYKGETKICLDQDKCEFCETCLDYCVNNAREIVG
                     QEYSVRDLVKEIEKDRIFYEESGGGVTLSGGEVMAQDMDFICGVINMCKSKGIHVAID
                     TCGYAKSENYERVAKCADLFLYDIKLIDEDKHIKFTGKSNDLILKNVKILSELGANIN
                     IRIPLIVGVNVDDENLEVKKMIEFLKPLNIQAVSLLPYHNIGKHKYDKIYKKYEGEEL
                     QRPSEEKLEEIKRLFEASNFNTKIGG"
     gene            10758..13127
                     /locus_tag="KR250_001626"
     CDS             10758..13127
                     /locus_tag="KR250_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HBH0067565.1"
                     /translation="MARGTFERTKKLREESINAEPHISIERAVLMTEAYKKYEGSVEI
                     PVLRALSFKHYIENRTLSINDGELIVGEKGDSPNGAPTYPEICCHTMEDLEVMHNRDI
                     INFSVSEEARKIHKEEIIPFWKKRQTRDKIINAMTPEWLAAYEAGMFTEFMEQRAPGH
                     TVCGDTIYKKGFLDLKKDIEARLKELDFLNDLDAYNKKADLEAMAIACDAMVILGKRY
                     AEKARQMAEEETDEAKKQDLLLIAETCDVVPAHKPETYHQAIQMYWFVHIGVTTELNI
                     WDAFTPGRLDQHLNPFYERDVENGILDRDRAQELLECLWVKFNNQPAPPKVGITLKES
                     STYTDFANINTGGINPDGQDGVNEVSYIILDVMDEMKLIQPSSNVQISKKTPQKFLKR
                     ACEISRKGWGQPAFYNTEAIVQELMEAGKTIEDARLGGTSGCVETGCFGKEAYVLTGY
                     MNIPKILELTLNNGYDPISKKQIGIETGDPRNFQSYEELFEAFKKQLHYMIDIKIEGN
                     AVIENICAKHMPCPLMSTIVDDCIEKGKDYQRGGARYNTRYIQGVGIGTITDSLTAIK
                     YNVFDKKKFDMDTLLKALDANFEGYEAILNLVSNKTPKYGNDDDYADEIMQEIFNAYY
                     NEVTGRPTVCGGEYRVDMLPTTCHIYFGEIMGASPNGRLCAKPVSEGISPEKGGDTNG
                     PTAVIKSCAKMDHIKTGGTLLNQRFAPSVVQGEKGLDNMANLVRAYFNMDGHHIQFNV
                     FDKNVLLEAQKNPQDYKDLIVRVAGYSDHFNNLSRTLQDEIIGRTEQTF"
     gene            13452..14255
                     /gene="proC"
                     /locus_tag="KR250_001627"
     CDS             13452..14255
                     /gene="proC"
                     /locus_tag="KR250_001627"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HBH0067566.1"
                     /translation="MKTLGFIGSGNMGSAMIGGIVQSGLVKAEDITVSDLSQVALDKV
                     KEEFGVNTTTDSKVAVTNSDVVIVALKPNICEKALTPLKELIDANKIIVSIAAGKTIE
                     SLESFIGSDKKIVRVMPNTPALVGEGMSALCKNSNVTDEELNMIKALFESFGEAEIVS
                     EYLMDTVTGISGSSPAYVFMFIEAMADAAVLAGMPRQQAYKFASQAVMGSAKMVLETG
                     KHPGELKDMVCSPAGTTIEAVKVLEEEGFRAAVIKSIVACIDKSKDMSK"
     gene            14853..17609
                     /locus_tag="KR250_001628"
     CDS             14853..17609
                     /locus_tag="KR250_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBH0067567.1"
                     /translation="MRDEILSFVNECVLFNSFDELKKESGIITEDIVKKFNLSRTQSS
                     RLLNDLVKSDNLIKINSRPVIFLPKNKINDVVGPTKKSIYENLDELKNECKLLKQETV
                     FNSIIGYDNSLKEVIEQIKTAVLYPNGGLTIMLTGESGVGKTFLAKIIYKYSVSSNVL
                     EKNSPYNVLNCAQYYNNLELLSSLLFGHVKGAYTGANESRAGLIEQSDGGVLFLDEVH
                     RLNSEGQEKLFTFMDTGTFSRIGENGVIRKARVRLIFATTESLSEFLQTFLRRIPINI
                     YVPNIEERGTIEKRKIVEHFIYEESKVLGLPIEITQITLNAILKIKFKGNIGECKNVI
                     KYACGRAYAKNQRRNGNIFVTLQDLPRNLYIENVDIFQFQSGKSKSIIFSPKQFSIEM
                     SNGRNKRNIIYQSFIKCLDYFDRLEKNEITKEKFSKKSSNLMIRLMDNLVFSNNDENN
                     NVLMELIISIMQDIFRNLEVNNNIKYDGNNVLAFSSYLYLKDESYQLSDEYKALENKL
                     LIYLKKESYKEYSIISKISTLITNRLDMYLNNVDTIIMIIFIKSTIIRMKFNQVNATI
                     IAHGYATASSMANVCNRMLGKNIFTAIDMPIDATIADVSKQMSRYLEGDRIKNGLIVL
                     FDMGSLNLIYEDLKNKINSPMLFIDQISTISALEVGNLLMQGKNIEEIAEIMDEETKP
                     NIQLYYPQKNKEYAIISSCFTGIGIAIKIQHLLSESLIGIVDVKIIPYDYDSLVSMGD
                     KEPVFEMYDVMAIVGTANPNVNGVDFILLEDIISGKGEDDVFRIFSRVVENEKIKNIN
                     DSIVKNFSLIRVIDSLTILDSKKIIERIEEGINAIENIQKKKLSNDKKISLYVHLSCM
                     IERLVRQTEIEEYTDMDLLIKNHHSEVKLIKNAFSVLEKSYSVQIPISEIGYIYNIIY
                     GLPQ"
     gene            17741..18220
                     /locus_tag="KR250_001629"
     CDS             17741..18220
                     /locus_tag="KR250_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBH0067568.1"
                     /translation="MVSLMRIDDRLIHGQVAYGWATSLGINVILVANDEAKNDQMKAM
                     SLNLAKPSNVTLYIRGVEESGEIVEKFASSKKSNVLVLVKNTKDALVVAKNSGGVLKE
                     INVGGLRYEEGKKKLTDLVAVDDDDINNFKQIGDLGIDIEFRMLPRDKKKQLSDLIK"
     gene            18236..18658
                     /locus_tag="KR250_001630"
     CDS             18236..18658
                     /locus_tag="KR250_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HBH0067569.1"
                     /translation="MKKFLIATHGELSRELVDTSKLIIGSLTNIEYFCMTTDKSADDA
                     EKEIRSILSNNKDSEEFVVLTDVFGGSVANICTNLLLQGFKFELLTGVNLPMLLSILL
                     LVEEDTKLIVKSGLEEAKNGIVYVNELLENQNKIKNVM"
     gene            18698..19489
                     /locus_tag="KR250_001631"
     CDS             18698..19489
                     /locus_tag="KR250_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBH0067570.1"
                     /translation="MLVQATMLAIIAGVGILDGRIFGQSMLDRPIVTGMLVGLVLGDI
                     KSGIMIGAQLELIWMGIAGIGAATPPDVVTGGVLGTAFAILSGNGAEVALAVAVPVAV
                     LAQSLGVLVRIINSYFSQKAVFYAKQADFKKVTIMMWLPVILFFLSTSVPTFLAIMIG
                     ADKVTALINAIPKVILDGLGVAGTLLPAVGFALLMDMLLSKKMSVFFFIGFLLASYGG
                     LDITAIALFGVCVAFVLNFYINNKENNQNPQLAINTLTEGEIDFE"
     gene            19482..20282
                     /locus_tag="KR250_001632"
     CDS             19482..20282
                     /locus_tag="KR250_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBH0067571.1"
                     /translation="MNKLTKKELRSMFWRSFALQGAFNYERMQNLGYCYSMLPAIKKL
                     YNKKENQAKAIERHLEIFNTTTVVVPAILGITAAMEEENANNPEFDESSISAVKTALM
                     GPLAGIGDSLFWGTFRIIAAGVGVSLAKEGNIFGPLLFLLLYNIPAFALRIFGLKYGY
                     QVGVNSLERIQREGLMEKIMSMATTVGLFVVGGMVATMLSITTPLKFNLNGAEVILQD
                     ILDKIIPNMLPLTFAFVIYYMLKRKVSVTKLTIGTIVTGIALHAIGLL"
     gene            20330..21385
                     /locus_tag="KR250_001633"
     CDS             20330..21385
                     /locus_tag="KR250_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBH0067572.1"
                     /translation="MTIQDYMLETPVRMREIISNADSLFNEVKRTNLKKIIITGSGTS
                     YHSGVQVQPYLQNLLDIDVVKMYPFMITEDTFKFDNENTLVVGVSQGGSSYSTYNAMK
                     LAEDKGCKIASMAGCKNALIDEISDYILTVNCGEEKSGAKTKGYYCTKLNLMLLGLQI
                     AREKGIISSEKYNEEINKILDAINRFEAVYKLSKQWIERNKEKLVNSKEIRIIGHSDI
                     YGDTLEAALKLLETMRIPVTGYEFEEFIHGIYNAINSDSTIFILDTGKEPRVTKMIDV
                     LSGWTENVFAIGRDVTENDKNLKIDITDNPYYQTFNFIVPIQLICGEIPTLRGVDPSV
                     PKDTRFHMKLGSKKLNK"
     gene            complement(21564..23510)
                     /gene="feoB"
                     /locus_tag="KR250_001634"
     CDS             complement(21564..23510)
                     /gene="feoB"
                     /locus_tag="KR250_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HBH0067573.1"
                     /translation="MINVALLGNPNVGKTTVFNLLTGSNQYVGNWPGVTIEKKEGFLG
                     KEIKVVDLPGIYAMDTFSNEEKVSKSYLENEDVDVIVNVVDASNLSRNLYLTTQLMQF
                     NKPIVILLNMLDIAESKGVNIDAKKLSEELGVIVVPIIAKKKNGVDRIEEEIKGATQK
                     AFIYQKNFGSETETYKKLESILSRCTKTTYTQKKSISDKIDNIVLNPILAYPIFIGVL
                     FLLFKFTFDWVGGPLQEGFASLIEAYISAPVSDMLSNSSPWFKSLIVDGIIGGVGGTL
                     PFFPLIFTLFFGISLFEDSGYMSRTAFLMDKVMRKVGLSGKAFIPIVMGMGCSSPAIM
                     ATRTLESEKDRRVTALIAPLMTCGAKLPIYALFVAIFFPHNAALVTTSLYLVGIVMAI
                     VVALFLNKTAFKTEAEPFILELPEYRIPTIDALIKNTWNKSKGFLIRVVTVMFAMSVV
                     IWGLSSFNVFGYTEDINVSFLATLGHIISPIFKPLGFDDWRTSVAILSGLGAKEIVVN
                     SLNILYGNLTVVLPTIFNGVTAYTFLIFTALYTPCIAALATMKKEYGNKMMFTSFAYQ
                     FILAWIMAFIVKSIGGVLFMGNSIMEALIGGIIIVLALFILFNKFKSTKNGNVCSGCS
                     ICSGCPSANSCSSKEDSNKKNNAH"
     gene            complement(23528..23746)
                     /locus_tag="KR250_001635"
     CDS             complement(23528..23746)
                     /locus_tag="KR250_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBH0067574.1"
                     /translation="MTVYDLKIGEKGFINSIDGDIKLSKRLLALGCINSTEIEVKKVA
                     PLGDPIIIRFRGFDLAIRKSDAKNISLQ"
     gene            23952..24461
                     /locus_tag="KR250_001636"
     CDS             23952..24461
                     /locus_tag="KR250_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HBH0067575.1"
                     /translation="MKTRKMTFLGLMIGYSLALYVLETYIPNPLIVIFPGAKLGLSNI
                     ITLISLMLLGVKDTVIIVTIRVILSSIFAGPLSYLLFSIGGAYLSLLFMYLASKIKDL
                     SLIGISIIGAIGHNIGQLIVASLIIENILAIGYLPFMLMASLVTGMFVGLVSRYTIPK
                     LKHFYSKFY"
     gene            24539..24700
                     /locus_tag="KR250_001637"
     CDS             24539..24700
                     /locus_tag="KR250_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl-phosphate phosphatase Spo0E family
                     protein"
                     /protein_id="HBH0067576.1"
                     /translation="MANNELEALKNEIEAVREEINTYIEYPEIFQEELVESSKKIDIL
                     INKYMYLSK"
     gene            24851..25516
                     /locus_tag="KR250_001638"
     CDS             24851..25516
                     /locus_tag="KR250_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HBH0067577.1"
                     /translation="MIDIGSKEIAIRLLLALIIGGIIGLEREKIRQFAGFRTHILIAI
                     GSCIVSITSIQLFFDYNLYTNADPARMPAQVLSGIGFLGAGAILKNKGGVKGLTTAAG
                     MWATACIGIAVGYGYYELAILGGLFVMIALFVLRIINTFLFSQEKNVLILKLSNLDSI
                     PFLYDKFEKNQLIVRNMEIECRNEEYWKVNFFVSYAKRIHLHDVMKEINMIEGVINVD
                     YLD"
     gene            25582..27366
                     /gene="pepF"
                     /locus_tag="KR250_001639"
     CDS             25582..27366
                     /gene="pepF"
                     /locus_tag="KR250_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HBH0067578.1"
                     /translation="MGTDRSTIEEKYKWKIDKMYSSKEEIEKDISKVKNLIQEVKEYK
                     GKLSESKENLYKVLNISENASRIVQNLYVYTHMKQHEDTRINDNQGRATKTEMLSTDL
                     GVATSYIVPEILAIEENKLSEYLKDEKLSFYTKYIKDILRDKPHTLSEREEEILAATS
                     ELSTIPENVYDMLAYADMEFPEIEDEEGNKVKLSHSNYSLFIKSKNSRVRKDAFEGEF
                     STYEKYKNTFASTLYGGIKSEIFYAKTRKYNSAIEASLFSDDVSLDVYNNLISAIDKS
                     IPNLNKYVELKKKFLGLNEIHMYDLYVPLTDKFDMDIPYDKAQDIILEALKPLGEEYL
                     KVIKSAFDEGWIDVYDNEGKKGGAYSWGSYDSHPYILMSYKNDLNSLFTLVHELGHSV
                     HSHYSRTSQPFLYSDYKIFVAEVASTLNELLLINYLLENSKSKDETIYLLNYYLEQFR
                     TTVHRQTMFAEFEKIVHQRVESGEPLTADEFTNIYYKLNEKYYGKSAIVDKQIGIEWA
                     RIPHFYSNFYVYKYATGFSAASALSQQILSEGSTAVDRYINFLKSGGSEYPLEQLKSA
                     GVDMTKKESIEEALNVFAELVNKLEKEL"
     gene            27618..28460
                     /locus_tag="KR250_001640"
     CDS             27618..28460
                     /locus_tag="KR250_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBH0067579.1"
                     /translation="MFKKKIGVLLASTMLVGALAVGCSSNSGGSGDSGDSSKVSVSGS
                     TSIGPLMETIGEKFQEKNQGVSVEVQQIGSSGGIKNAIEGTSEIGMSSRDLKDEEKSA
                     GLKETKIAIDGIALITNTANSVKNLTMDQVKGIYTGKITNWKEVGGNDAPIVIVSREE
                     GSGTRDGFQEIVGFDSEELTKDAQISDGSGNIKTTVEGNENAIGYISFGYVDEKVNSL
                     KINDVEATDANVIANKYPISRPFILVNKEDGLSEKGKALIDYILSDEGQALVSEKGFI
                     KVNQ"
     regulatory      28693..28776
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            29356..30033
                     /locus_tag="KR250_001641"
     CDS             29356..30033
                     /locus_tag="KR250_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0067580.1"
                     /translation="MYNLLVVDDDVDILNINQIYFSNIGFNVYTSETAKDAMDIVESI
                     VLDCIILDISLPDIDGYKMCNDIKEKVNIPIIFLSNYEHEEEKVRGFLAGGDDYITKP
                     YSLKELELRIYARMRQYKNITSVPNVLKFSALVINVNSRKVTFDGESIDLTTMEFEIL
                     LFLAENKEQVFSKIEIYNQVWKMPDIGDQHTVQVHIAQMRKKINSLSREHQYIQTVWG
                     KGYKFVP"
     gene            30105..32120
                     /locus_tag="KR250_001642"
     CDS             30105..32120
                     /locus_tag="KR250_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBH0067581.1"
                     /translation="MKKYNILKGTLIVLIVSLLAITGLNLLYKYDNKYTYKSIRASNG
                     ILTIDNDTFTKGKLVFLIDDWEFYNHKIFKPNDFTEQDIKPEYVFIGQYPDFSMRKGT
                     QSPYGNATYRMRIKNEGKDKLLSLELPEIFSASEVWINGEMVSKLGDVGTEKYRPKIK
                     NSVVSFIAKKNTEIIINVSNFSHYYSGLYYPPALGETKDISNMMFYRLLFYSIICFTT
                     FAIAIFSLSVWFLSNKSKIYLYFGCMCVFFAIHVSYPFVHLLGLPLVSFTYAVEDLSY
                     FMVVLCIICINGSISKIEENKVYRFLILPLGITMSIISIVIPIILLPYDMYFVNIYGK
                     LIDIYKYIVFIYILMSSLVSIYKKTCSEHISSYILVSVNTVFGISILFDAVTSNRFEP
                     IFTGWQTEYCGFVIVILFSVMMINRNRIIIKENEKLTRNLEHEVEVRTNELTTLLNER
                     KRFLADVAHDLKAPVSAIQAFIDLIKVGNIHVDEETRTYLYAINQKSNEVQNRVRSLQ
                     EFTSQDKSIGISEKICLNNFIKEIYENNLPDTQACGVNFETYVLEKKIYAKINKEMLT
                     RAFENLIYNALTFTPFDGKIILSMDKEDGFAIIKISDNGKGIPPENIPKVFDRFFTER
                     DNNEPKGQGLGLYIVKSIIREHGGEIFVDSELGKGTVFTIKLSLCFL"
     gene            32170..32850
                     /locus_tag="KR250_001643"
     CDS             32170..32850
                     /locus_tag="KR250_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0067582.1"
                     /translation="MQTKILIIDGDKDNCQKLKGFLEEKGISIDLAYNCEEAIGKIFS
                     NKYDLIFLEIILSDGDGWTLCKKIRNVTTCPIVYMTYINEDQSILNALNSGGDDYLIK
                     PLNLEILYAKVKAILRRMNSYVNNNENNTKLEEYNRTSGVIKLEDKIIKLTPTENKLL
                     NFFIENPEKTLTTKEIYEHVWMNEYLEDNYSVVVAVNGLRKKIEKDYKNPQKIITIRG
                     AGYYFNKE"
     gene            33366..33812
                     /locus_tag="KR250_001644"
     CDS             33366..33812
                     /locus_tag="KR250_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0067583.1"
                     /translation="MAISDLAFGTECLKTSFQMLFSVISLVNINLLQAQILIAIGGLI
                     SILAMVSLTLFLSAKCKDSLTSMLIAFSICLIPMGIGVSWIGYLLPPGGIGFKISLLY
                     QMTSFNFLHLGGMSFWTPHIILFFSVIEIPIFIFLAIRTYCKHQVP"
     gene            34193..35110
                     /gene="pstC"
                     /locus_tag="KR250_001645"
     CDS             34193..35110
                     /gene="pstC"
                     /locus_tag="KR250_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HBH0067584.1"
                     /translation="MIVKSRLVGLEKNDNNGNKTKYVIEKVAKNIFLLSALVAVASLL
                     LIIGFVFYKGLRPFIFEGYSFIDFLTGADWVPSANKFGISSMIVASIFATLGALVIGV
                     PVGILTSVFIAEVAPKRLAKIMSPAVELLAGIPSVLYGVFGLAVIVPTIQGIFNLPKG
                     QSLLAIVIVLAVMMLPTIISVSETAIRAVPKAYKEGSLALGASKIETIFKVVLPAAKS
                     GVLAAVVLGVGRALGETMAVILVAGNSPVMPTAITDSVRPLTTNIALEMGYAFGTHQE
                     MLFATGVVLFTFILILNLVLNKLSNKAVN"
     gene            35112..35936
                     /gene="pstA"
                     /locus_tag="KR250_001646"
     CDS             35112..35936
                     /gene="pstA"
                     /locus_tag="KR250_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HBH0067585.1"
                     /translation="MRKFKENLLKSLVYLSALFTIVSLVVIVGFIFIKGIGNMNFNFL
                     FSNYSASGDGGILPMIITTLYTVVVSIVVATPIGILAAIYLQEYAKKGKAVTAIRFAT
                     ESLSGIPSIVYGLFGGIFFVVTLKMGYSIVAGSLTVAIIILPVIIRTTEEALKTVPQA
                     YRESSLALGATKFQTLYKVVLPSAIPGILSGVILSVGRIIGESAAILLTAGTVAKMPG
                     GIFDSARTLTVHSYLLTKESGDIATAASIGIVLIVIVLALNMLARFVAKKLNKANY"
     gene            35955..36719
                     /gene="pstB"
                     /locus_tag="KR250_001647"
     CDS             35955..36719
                     /gene="pstB"
                     /locus_tag="KR250_001647"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HBH0067586.1"
                     /translation="MELIDKIKMSVKDLDLFYGDKQALKKINMDIKENKVTALIGPSG
                     CGKSTFIRTLNRMNDLIEDVTIKGNISVDGEDIYTSDDVINLRTKVGMVFQKPNPFPM
                     SIYDNVAYGPRTHGLRDKKQLDKIVEESLKGAAIWDEVKDRLKSSALGLSGGQQQRIC
                     IARAIAMRPEVILMDEPTSALDPISTLKVEELIEDLKKDYTIVIVTHNMQQAARISDE
                     TAFFLNGEVIEFSDTKTMFTTPVDKRTEDYITGRFG"
     gene            36747..37400
                     /gene="phoU"
                     /locus_tag="KR250_001648"
     CDS             36747..37400
                     /gene="phoU"
                     /locus_tag="KR250_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HBH0067587.1"
                     /translation="MVNNSLDISIDTLKEQTVLMMEKCEEAIEKSVTCMIEKDLEGAR
                     KVVEDDEEINKLREEIRDKSIELIVLKQPMARDLRFIYSLGNISIELERIGDYAANIA
                     REMLKIGGEAYIKELIDIPKMAKECKSMMSQTREALILFDASIAYDVGTKDDIVDGLY
                     EQVQIDCLKIMHENSNTIDQGVKLLLIGRYLERIGDHITNVCEKIIFAIEGKMVEIG"
     gene            37558..38427
                     /locus_tag="KR250_001649"
     CDS             37558..38427
                     /locus_tag="KR250_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBH0067588.1"
                     /translation="MKLQKMGMRTIKTGISVTLCMILGDYLVENMFYSLVACIISVQD
                     TVKGSLKTGINRVEGTILGGIIGFIFAIIKAGDPFLCGLGVMCSIYLCNLFKIKSVVV
                     ACVTFLAIHLGVGNSDPVYYSLHRVLDTSVGVLFGVLVNYYVARPNYLNSVIDEFKKI
                     EELSMLLIEDKILRKENLNVEKFEEEVEKLEEIYSKLIDELDYSLSETNIEKIENALR
                     ICREIHFHMQSIELLENKLYLKEHTYKYLKKTYNVDKLDWNIDEEKSPVFNYHLMKIM
                     IEIELLRKLNKSY"
     gene            38514..39950
                     /locus_tag="KR250_001650"
     CDS             38514..39950
                     /locus_tag="KR250_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase"
                     /protein_id="HBH0067589.1"
                     /translation="MNTRNDNSKILEIYKMKLFGELVKAVWNDDIEDIENLPNKILNE
                     DLKDNLNNYFSGKFDKDTMVNLIRVTMGLDVTEDTDKNLKESLHEAVNLDEVKRPIIS
                     VISDACKYCDENNKEDESCKIRSKHINCNEEDVCSACGDCVSRCKLGAISDKIEFIPM
                     IKLLKDIKSPVYAIVAPAFVGQFGKDVSPGKLRTALKKIGFEDMIEVALAADMLTAKE
                     AYDYYEHMKKDEEGCFITSCCCPIWVSLIQNSFPQILENVSPSVSPMIACGRAVKILN
                     PDAKVVFIGPCMAKKKEAMLEDIKDAVDFVLTFKELQEIFDALELNPAIMDESNRVEA
                     SLSGRVYARTGGVSKAVKLSVRRIDKNIKFEEKAFEGTKECMDGLKKVLNKEIEATFI
                     EGMGCVGGCVGGPKRILSVEEGTKNVDEYSESTNMETPFDNLNVLQFLTSLGIKRVES
                     LGEKEEEKVKKIFSRDIKSNKKENKYKK"
     gene            39998..40825
                     /locus_tag="KR250_001651"
     CDS             39998..40825
                     /locus_tag="KR250_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBH0067590.1"
                     /translation="MKIKDNKAVFHVFIRIFLIAVLILTGTLLEEKINNVPVENISSK
                     GENTLELLSQSNTLGEIIYKASQEETKGPGATSEKVAYITIDDGPSKYTNALLDILKE
                     NDVKATFFMLNQNMKNHKEEVRRVLEEENSIGFHSVSHDIHKLYKSPEVTVGEFNTCN
                     NTLQEITGQSSKLIRLPYGSKPYMPEGSYNKLMANDYLIWDWNLDTQDWKSSTSQIVS
                     NILYYGRKRDELVVLIHEKEQSLNALNNIIRILKERGYTILPITENITPKNFWQENL"
     misc_binding    41121..41383
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            41459..44125
                     /locus_tag="KR250_001652"
     CDS             41459..44125
                     /locus_tag="KR250_001652"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HBH0067591.1"
                     /translation="MENTNLSKTYNPKDFEARLYKKWMDEGYFKSKPNPDKKPFTIMM
                     PPPNITGQLHMGHALDHTLQDILIRWKRMDGYEAFWLPGTDHASIATEVKVVERIKKQ
                     EGKTKYEIGREEFLKRAWEWKDEFGGKISNQLKQLGDSCDWDKERFTMDEGCNEAVIE
                     FFVSLYEKGHIYRGNRIINWCPDCKTTLSDAEVEHEEHDGNFYHIKYPLKDSEDFLEI
                     ATTRPETMIGDTGIAVNPEDDRYKHLIGKTAILPLVGRELPIVADSYVDLEFGTGAVK
                     MTPAHDPNDFEVGLRHNLEQLNTMNEDGTMNEVCGKYEGMDRFECRKAIVADLKEQGY
                     LIKIKEHNHNVGTCYRCHTVVEPRLSEQWFVKMEELAKPAIDILKKGELEFVPDKFDK
                     TYLQWLENIRDWCISRQLWWGHQIPAYYCQECGEIVVARKMPDKCPKCGSTHFKQDED
                     ALDTWFSSALWPFSTLGWPNKTEALDYYYPTSVLVTGYDIIFFWVVRMAFAGMFCMNE
                     KPFDHVLVHGLVRDSQGRKMSKSLGNGIDPLEIIEQYGADALRFTLTTGNSPGNDMRF
                     YMERVEFARNFANKLWNASRFVFMNIDEDIIKNMTRESVKEDLTLADKWIISRANNIV
                     KEATNNMDKFDLGIALQKIYDFTWSEYCDWYIEMVKPRLYGEDANAKSAALYTLTYVL
                     EKILKLLHPYMPFITEEIYTHLPTVEGCIIVSEWPKYNEEDNMAEEEDMMNLLMEGIR
                     SIRNVRAEMNVPPSKKAKLIIIPSEEKIEAIELGKDYFITLASASNVEIAKDKSNVPE
                     DAVGVVIDGVEIFIPLNELVDFEKEIERLSKEKKKLEGEIKRVNGKLANQGFLAKAPE
                     SLIEEEKAKKEKFEEMIKSVEERLTNLESKIK"
     gene            44550..45260
                     /gene="rgaR"
                     /locus_tag="KR250_001653"
     CDS             44550..45260
                     /gene="rgaR"
                     /locus_tag="KR250_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RgaR"
                     /protein_id="HBH0067592.1"
                     /translation="MFRVVICDDEKIQRSILKEFTEKLLSERCLNYQILEFSCGEDLI
                     SKYPEKIDIIFLDIQMKDINGIEVAKKIRKFDEKVEIIFTTAFSEYAPQGYEVRAYRY
                     LVKPIEYSNFAMGVNLCIDNLQRKKERYIVLNSKKGFNRILIDSILYVETVKRDLVVH
                     TIGRDYKANMSMKQAEKLLCENGFFRCHTSFIVNLRRIEDLRDNTITINEQFIPISKY
                     RLKDLKVALTSLLCDIMY"
BASE COUNT        18168 a         4807 c         8113 g        14480 t
ORIGIN      
        1 ttgaagaata ctttaaatat acagataaat ctattgaagt aaatccatta agattaaaag
       61 ataaagtaaa agtagaaaat aattttgatg cagatttaga agacaatagg ataaattata
      121 taagtaatat aggaatgatt atttaagtga aagtggataa aacgataaat tttcttaatg
      181 gatttaaaaa aactaaacca tgtaataaaa tctataaaat aataatagtc ttaattttaa
      241 cgtgtatttt atttaaatca tttatgtatg tgaaatctat aaatgaactt gaagattata
      301 taagtcaaaa taagctagtg gataaatcaa gacacacttt accaacaaca gataaaacta
      361 gagagaaatt ttctaaaata aaacttaatg aaataaaaaa aatatatgaa acaattggaa
      421 taaatattat tactaaaata tcgatagata aaaataagct agagattgaa ggtgtgtgta
      481 gtgatttaga tgttttaaac aatctaagga aaataaagta cataaaatat tattacatag
      541 acagtattgt aagagatgga gaatattata gatttagaat aaaaagttag atgggtgatt
      601 atttttgaga ttaaataata gagaaaaaaa tctacttaaa atattgttaa tattagttat
      661 tatattggca agtgatatag cagtaagtta tttggaagaa aacttaacta gtttgaagaa
      721 tgaaaagcaa aatataataa ataaaataaa taatcaagat aattatcaaa taaaggataa
      781 taagcatata gatgataaat atatagaaag tgttgaatca aataaaaatg aagaattaaa
      841 aaataatttt aataactcat ttttagataa aaaaacaaat aaagatatag tgttattagt
      901 agaaaatgaa ctgagagata ttgtgaatgt agaatatatt aatcaagaaa gtagtttaga
      961 taatcacaat aaaaaacaag ttaatttaga aataaaagtt aatggagaac ttgcaaattt
     1021 gttgaaactt gaaggtgtaa tgaagaagtt aagactaaat aataaacttg aaaagataga
     1081 aattaataaa ttaaaagtca ataattttga tgttcaaaat attccaaatg aatataaaca
     1141 agaaaaagac aatattgttg aatgtaaaat gacagttaga gtcatataac aaaaaagaat
     1201 agataaagta aaaaactatt atcaattaat aaaattttat agaggaatta gttttacgat
     1261 aattcatttc tacaagaggg attgttgatg attttatcga aatcctaatt aaagttaata
     1321 tattgtacca aaatataagt gaatttaata gaatatttac ttatactata gattgattaa
     1381 taaaaaaatg gtataataac aaattgtact ttaatttaat aataagggag tggaagtcat
     1441 attgttaggg agaaatgagc tatgcccttg tggaagtggc aaaaaataca agaaatgttg
     1501 tttaaataaa gatattgttt cagaaagagc atgcagaaaa actgaacttt cacaaaaaca
     1561 atatacagat ttatattcaa agttatatga ttattcaaga caggataagt ttaaagaaga
     1621 atgtgaaaaa gctaaagaaa tgttttatat aatgcaaaat gatagtgtaa atgaaaaatt
     1681 tgaaagattt tttaatactt atttaataca agaccacata atggaaaata aaaaggttat
     1741 gacagttggt tttcttgaag aaaatggtgc aaacctaagt caatcagaag tatcaatatt
     1801 aaaaagtatg tttgaatcat acgtaagtat ttatgaggta aaagaagttt ctgctggaaa
     1861 aatagtatta aaggactgtt taagtggaaa tgaactttgt acagaagatg taaaacttct
     1921 gagaagtttt aaaatcggaa gttgtatgat agctagaata gttgatattg aaggaactaa
     1981 catattgata gatataacta taagtatatc aaatgaggtt aaagatataa ttttaaagga
     2041 tattatgaat ctattcaatc aatatcaaga tgtatacaaa gatatgaagt cgttcttaat
     2101 ttatcataca catatactat acaagtatat acaacagctt ttagacccaa gtatagctga
     2161 atatttaaag agagaaagag ataaaaacga tacaaaagtt aattcagaaa atgaattagc
     2221 tgaagaagaa tgtaaagttt tagatactct taaagaaaat gtagaaaaag aaaatataga
     2281 tgcatgtata gagttttgga atgaatataa gaaaaatcat gaaaatataa aaggagctga
     2341 aaatggttgg gcagctgctg ttgaatacta tgttaaaaaa gaagcaggac aacctataac
     2401 tcaagttcaa atatcaaaga aatacgatat aagctctagt acactaggaa agagatacaa
     2461 ggatttaaaa atatcttaat taattataaa aagctttaag tcatagataa agaatctata
     2521 gtataatata tgtataatgt ttagaaagag gtattaataa tgagtcttag tttaggaaat
     2581 gcaattgctg tatcaacgaa ctttataaaa tctaacccag ctgtacctgt gtacaatgga
     2641 gaagaaatat atgcagttat ttatcatata gaaataggat ataataatgg tgtatcacct
     2701 gataccaaac aatatataat aatagactat aaagagtttg atttatctat ggataaaaaa
     2761 aatgaactaa taaatattgc taaagagtac tgtaaatcta aaggtattgt agaaaacgaa
     2821 aaccttgaaa taaacctttt agacaatgaa gaagcagaga acttcttaga aaaggtgttt
     2881 aataatatga aggttataag aggtcttata actaaaaaag gataaataaa tggactatat
     2941 aggaatagaa aatataacac cttatgaaaa tacatatgaa tttagtgtat atgaatatga
     3001 tgatgaaatc accttaggta gtgaaaagtt atatgtatgt gaattaaggg ttgtattgat
     3061 taaagttaat tctctgtatg ttgaaagatt gcataaatca gttgaagcaa tggtcttagt
     3121 aaaaaacttg aaaaaagatt tagataaaac acttgttgta aacaaaataa agaattttgt
     3181 gctagatgag atttgggtag aaaatctagt aaaagagaat atagaagtta tatttgtaga
     3241 aagctaggaa taaccctagc ttttttatat tacaatctaa gagaggaaaa caatatgtta
     3301 agagtatcta atgtaaaact tggaattgat aaagatatat ctttattaaa agatgttata
     3361 cttaagaagc taaaaataaa agaaaaaaac ttagtcaaat atagtatata taaagaatct
     3421 atagatgcta gaaaaaaagg aaaaatggaa tttgtttatt ctgtagatgt agaagttaaa
     3481 gatgaaaata aaatacttaa ctcaaaaata aaagatgtaa caaaaataaa ggaaatcaga
     3541 tacgtaaatg tacctgtggg tgataaaaaa ttaaagaata atcctttggt aattggtagt
     3601 ggaccagctg gtttgtttgc aggtctgctt ttagctcaaa tgggttatga accaatcata
     3661 cttgaaagag gtcttgatgt agacagtaga accaaagata taagtgattt ttggacaact
     3721 agaaaattaa aaaataattc aaacgttcaa tttggagaag gtggagcagg aactttttcg
     3781 gatggtaaac ttacaactag aataaaagat atacgatgta gaaaagtatt agaagagtta
     3841 gttcagtttg gggcaccaga tgaaatatta tactcacata agccacatgt tggaacagat
     3901 atattaaaaa atgtagttaa aaatataaga aatgaaataa taagtttagg tggtcaagtt
     3961 agatttaatt caaaagtaac agatatagtt gtatcaaatg attctataca atctgtaata
     4021 ataaatgata ctgaaaaaat agatacagat acaattatat tagctgttgg acatagtgca
     4081 agagatactt atgaaatgtt gtattcaaga ggtataaaaa tagttcaaaa accttttgca
     4141 ataggagcta gagttgaaca tccacaagat ttaataaata aagcacagta caaggatttt
     4201 tataatcacc ctaggttagg agctgcagat tatagactta tagagcatgt ttcaaatgga
     4261 agaacagcat atactttttg tatgtgtcca ggaggtagtg tcatagcatc tgcatcaaat
     4321 gattttgaaa tagttacaaa tggtatgagt gaacatgcaa gagataaaat aaatgcaaat
     4381 agtgcatttt tagtaaatgt aacgccagaa gattttaaga gtgataatcc actagcaggt
     4441 gttcatttcc agcagaagta tgaaaaatta gcttttgaat taggtggaaa aaattataat
     4501 gcaccagttc agcttgttgg agattttata aacaataaga cttctttgaa tttaggaagt
     4561 gttgagccat catatagacc aggatataca cttacaaatt taagtgaatg tttgcctagc
     4621 ttcgtaacag agactatgag agaagcgttt atatctcttg ataaaaaatt gaatgggttt
     4681 gcaatgtatg atgctgtttt gacgggagta gaaactaggt catcatcacc aataagaata
     4741 gtgagagatg aaaatacttt agaatcagtg agtattaaga atctatatcc atgtggtgaa
     4801 ggtgctggat atgcaggtgg tatagttaca gcagcagttg atggtataaa atgtgcagaa
     4861 aaaataatac aaaaatacac caataataca aaaatttaac ataaacttaa tgatttttta
     4921 atatttaaca tgaatttaac attatcactc ctataatttg ataaaataca ataggttata
     4981 aaatttttag atacagtaat ctctttacaa ggaggaaata atttgaacat tataataagt
     5041 ctaatgggtg gattaggtct gttcttatat ggtatgaacc ttatgggaga aggtcttcaa
     5101 aaatctgctg ggacaaagct aaaaaagata ataaaactac ttactagtaa cctatttatg
     5161 ggagttttgg taggtacagg agttacagca gtaatacaaa gttcaagtgc tactactgta
     5221 atggttgttg gtttcgtaaa tgcaggaata atgactttaa aacaggcaat aggagttata
     5281 atgggtgcta atatagggac tactgttaca gcccaattgg tatctttcga cttaactggt
     5341 atggccccag tagcattagg tgtaggtatt atactttatt tatttggaaa taagcctaga
     5401 ataaaaaata tagccgaaat acttataggt tttggtatat tatttacagg tatggatttt
     5461 atgaagatgg cagtggagcc actaagagac tatcaaggat ttactgactt acttgttaca
     5521 tttggtagat atccactact tggattatta ttgggatttg ggattacagc aataatacaa
     5581 agttcaagtg catctatggg tatgttggtt gcactggcag cagaaggtct agtgccacta
     5641 tcagcagcac tgccaattct ttatggccaa aatattggta catgtgtaac ttcactgtta
     5701 tcaagtattg gtgctaacaa aaatgcacga agagcagcta tgatgcatct aatcttcaat
     5761 gttttaggaa cggtgatatt cttaatattc ttgaataaac cagtagtaag catggttaca
     5821 agttgggatc caagcaatgt tgcaagacaa atagccaata cacatacatt gtttaatata
     5881 atatctgtat tgatactttt accatttaca agtcttatta taaaactagc tataaagctt
     5941 gttcctgata gagcaggtga tatagatgaa gatgaaacta aaactattaa atatatagat
     6001 gatagaatga tagaaactcc ttctatagct cttgcaaata cgataaaaga agcacttcgt
     6061 atgggtgaga aggcgaagga aagcctaaat gcatctatgg aagctttagt agaacattca
     6121 actgaaaaaa tagataagac atatagacga gagagattaa tcaatgattt acagaaagct
     6181 attttaaatt atttacttaa actttcaaaa gcagctttag atgatgattc aagagaagtt
     6241 gtggatactt tgtttaatac agtcaatgat atagaaagaa ttggtgacca cgctaaaaat
     6301 atagctgaat tatcacaagt tgcaatagat tcaaatattt atttctcaga agaaggacaa
     6361 tctgaacttg atgttatgta taatagagtt gtttcagcat atacttatgc attagaatca
     6421 atgaggagcg ataatgttga tttagcttgc aaggttataa aaattgaaga acaagttgat
     6481 ataatggaga aatcttgtag agctaatcac atgtatagat tgaataataa tctatgtagt
     6541 atagaaaatg gagttattta tttagatgta ataagtaatt tagagagaat atctgaccat
     6601 gctgtcaata tagcacaaca agttatagcg aagagattag gaaatgatta aaaataatta
     6661 atttaaaata atataaaatt taaaatagag gataaataaa agagaaactt taaatgatac
     6721 gtttagagtt tctttttttg taaatttatt tgactaaaat ttttaaattt atacatagtt
     6781 atagtattta gaagaatcga tttcctgata ataaatgatg agatatgtat gattttgtgg
     6841 caaactttta aaaaaaacac ctatattttg tgttataatc tctttaaaac gaatatgctg
     6901 aaaagaggga tttaaatggg aaaaataaat tttgattata gtaaagctac agagtttttt
     6961 tgtcaaaacg aaatagatgt catgcaacca tatgtagatg ttgcgcacga catgttacat
     7021 aataaaactg gtttaggaaa tactttttta ggatggatag accttcctaa aaattatgat
     7081 aaagaagagt ttgacagaat aaaaaaatct gctgataaga taaaatcaga ctcagatgtt
     7141 cttttggtaa taggtatagg aggctcttat ttaggttcga gagctgctat agatatggta
     7201 agtcattctt ttaggaatgg acttaaaaaa gagcaaagaa aggctccaga agtatatttt
     7261 gtagggcata atataagctc aacatatatt atggatttat tagatataat tgaaggaaag
     7321 gatatttctg taaatgtaat atctaaatca ggaactacta cagaaccagc tctggctttt
     7381 agaatattta aagattactt agagaaaaag tatggtaagg aagaagcaag aaagagaatt
     7441 tatgcaacaa cagatgcaag taaaggtgca cttagacagt tagctacaga agaaggatat
     7501 gagacttttg taataccaga tgatgtaggt ggtcgttttt ctgtattgac agcagttgga
     7561 ttacttccta ttgctgctgc tggacttgat attgatgcta tgatgaaagg cgcaaatgat
     7621 gctagagaag catttcaaaa tcctgactta aaaagtaatg atagttatag atatgctgtt
     7681 gctagaacaa ttttacatag aaaaggaaaa gatgtagagt tattagtgaa ctatgaacct
     7741 caactacatt atgtaagtga gtggtggaaa cagctttatg gagaaagtga aggaaaagaa
     7801 aataaaggtt tattccctgc ttctgtagat ttctcaacag atttacattc tatgggtcaa
     7861 tacatacaag atggaaaaag gcttttattt gagacagtac ttaatgttga aaactgtaag
     7921 agaaatataa ctataagttc agaggaagta gacttagatg gtttaaatta tttagcaggt
     7981 aagactgtag attttgtaaa tcataaagct tttgaaggaa ctctattggc tcatacagat
     8041 ggaaaagtac ctaatttagt aataaatata cctcaactag atgagtataa ttttggatat
     8101 ttagtttatt tctttgagaa ggcttgtgga ataagtggat atttattggg agttaatcca
     8161 tttgaccaac caggagtaga agcttacaag aaaaatatgt ttgctttact aggcaaacca
     8221 ggttatgaaa aagaaaagga agagctagaa aaaagactta aataatttta aatagttaag
     8281 gtttaattaa gattaaaata atataatgaa atcatcaaaa aggagtttac tcaaaataga
     8341 tgatttcatt ttttttatga aaaattggga aaaatatatt ttgataattt atattatatt
     8401 aagataggga gtggtatgag atgtcaagaa gaaagaaagg gataagttta gtaatattag
     8461 ttgctataat aagttcaata cttagtagtt ttctaacaat aattttagtt aaggacaatt
     8521 tagtaagcaa atctacagga agttctacgc cgatagttgt aaatgatgat ggtaagagtc
     8581 aaaatattta tcaagcagtt gcagaaaaag ctacgccatc tgttgtaggt attacaacaa
     8641 ctagtgttga tactagtaat atgtttgcaa taccaactga aacacaaggt gtaggaactg
     8701 gaatcatagt tgattcaaat ggatatatac ttactaattc tcatgttata tcagatggtc
     8761 aagctacaag tgtaaatgta ttattcaatg atggttcaac aacttcaggt aaagtagtat
     8821 ggtttgacca acaacttgat ttagctatag ttaaggttga taaaactgga ctaacaccag
     8881 cagaatttgc tgatagtgac aaagtaaaag ttggagatat atcaatagca ataggtaatc
     8941 ctcttggatt agattttcaa aaaacagtta ctcaagggat aataagtgga ttagatagaa
     9001 ctatacaaac agaaaaaact aatatgactg gtttattaca aactgatgca agtataaatg
     9061 caggaaatag tggaggacca ttgttaaatc aaaaaggtca agtaattggt ataaacacag
     9121 caaaagcttc tcaagcagaa ggattaggat ttgcaatacc aatcaataca gctaagagta
     9181 tagttgaaga agtaatcaaa aatggaaaat atgaaaaagt aactttaggc ataaaaggta
     9241 cagatgtatc aaattatgaa gcagcaacag gtacaaaatt aagtactgat aaaggtgttt
     9301 atgttgcaga agtaatatct ggttcatcag cagaaaaagc tggagtaaag gtaggggata
     9361 ttataactaa agtaggagat acagatataa caggtatgaa tgatttaaat aaaaagttgt
     9421 atactttctc aaaaggtgct agtacaaaaa taacagtaaa tagaggcgga aaagcagtaa
     9481 caataaatgt gaatttctaa atatgaccaa aaagatagag actacatggt aaagtgtagt
     9541 ctctattttt atttaatttt tatagtatat acatctaaat taattaaaat gatgtattgt
     9601 ttatagttta ttgctatatt ttcttataaa aataaattta ataaatattt aaatttaatt
     9661 tacaaaaaca atataaatat cataataaaa taaaattttt taatattact aaaaatatat
     9721 agacaaaata ataacaaata tagtattatg taattgtaga aatgcttttc ataggtatat
     9781 tctatgaaga tgaattgcat aaaaatgaaa aaatggaatg gatattattt aggggtgaaa
     9841 aatcatgaat ccattagtta taaacttaca aaaatgtagc atacatgatg gaccagggat
     9901 aagaagtaca gtatttttta aagggtgtcc acttgaatgt gtttggtgtc ataatccaga
     9961 gagtcaaaca tatacaaaac aagttttgta taatgaagaa agatgttcaa aatgtgaagc
    10021 atgtataaat atatgccctc ataaggcaat ttacaaaggt gaaacaaaaa tatgcttaga
    10081 ccaagataag tgtgagtttt gtgaaacttg cttagactat tgtgtaaaca atgcaagaga
    10141 gattgttggt caagagtatt ctgtaagaga tttggttaaa gagatagaga aggatagaat
    10201 tttctatgaa gaatcaggtg gtggagtaac tctatcaggt ggagaagtaa tggctcaaga
    10261 tatggacttc atttgtggag taataaacat gtgcaaatca aagggaatcc atgtagcaat
    10321 tgatacatgt ggctatgcaa agtcagaaaa ttatgaaaga gttgctaaat gtgcagattt
    10381 gtttttatat gatattaagc ttattgatga agataagcac ataaaattta caggaaaatc
    10441 aaatgactta atactgaaaa atgttaagat tttaagtgaa ttaggggcta acataaatat
    10501 aagaatacca ttaattgtag gagtaaatgt agatgatgaa aatttagagg ttaaaaaaat
    10561 gatagagttt ttaaagcctt tgaatataca agcagtaagt cttcttccat atcataatat
    10621 tggtaaacac aagtatgata aaatttataa aaagtatgaa ggagaagaac tacaaagacc
    10681 atcagaagaa aaattagagg aaataaaaag actgtttgaa gcaagtaatt tcaatactaa
    10741 gattggaggg taataaaatg gcaagaggaa cttttgagag aactaaaaaa ttaagagaag
    10801 aaagtattaa tgcagaacca cacatatcta ttgaaagagc agtacttatg acagaagctt
    10861 ataaaaaata tgagggaagt gtagagattc ctgtacttcg tgctttatca tttaagcatt
    10921 atattgagaa tagaactctt agcataaatg atggagaatt aatagtaggt gaaaaaggtg
    10981 attctcctaa tggagcacca acatatccag aaatttgttg tcatacaatg gaagatttag
    11041 aagtaatgca caatagagat ataataaatt ttagcgtatc agaagaagct agaaaaatac
    11101 ataaagaaga aataattcct ttttggaaaa agagacaaac aagagataag ataataaatg
    11161 caatgacacc tgagtggtta gcagcatatg aagcaggtat gtttacagag tttatggaac
    11221 aaagagcccc aggacataca gtttgtggag atacaatata taaaaaagga tttttagatt
    11281 taaagaaaga catagaagca agattaaaag agctagattt tcttaatgat ttagatgcat
    11341 ataataaaaa agcagattta gaagctatgg caatagcttg tgatgcaatg gttatattag
    11401 gtaaaagata tgctgagaaa gcaagacaaa tggcagaaga agaaacagat gaagctaaga
    11461 aacaagattt attactaatt gctgaaactt gtgatgttgt accagctcac aaaccagaaa
    11521 cttatcatca agctatacaa atgtattggt ttgttcatat aggggttact actgaactta
    11581 atatatggga tgcttttact ccaggaagac ttgaccaaca tctaaatcct ttctatgaaa
    11641 gagatgtaga aaatggaata ctagatagag atagagcaca agaattactt gaatgtttat
    11701 gggttaaatt taacaatcag ccagcaccac caaaagttgg tataacatta aaagaaagta
    11761 gcacatatac agattttgca aatataaaca ctggtggaat aaatccagat ggtcaagatg
    11821 gtgttaatga agttagttat ataatacttg atgttatgga tgaaatgaaa ttgatacaac
    11881 ctagttcaaa tgttcaaata agtaaaaaga ctcctcaaaa attcttaaag agagcttgtg
    11941 aaatatcaag aaaaggatgg ggtcaaccag cattttataa tactgaagca atagttcaag
    12001 agcttatgga agctggaaaa actatagaag atgcaagact tggtggaacc agtggttgtg
    12061 ttgaaactgg atgttttggt aaagaagcat atgttctaac tggatatatg aacattccaa
    12121 aaatacttga attaacatta aataatggat atgacccaat tagtaaaaaa caaataggta
    12181 ttgaaactgg agaccctaga aatttccaat cttatgagga actatttgaa gcatttaaaa
    12241 agcaattaca ttatatgatt gacataaaaa tagaaggaaa tgctgtgata gaaaatatat
    12301 gtgcaaaaca catgccttgt ccacttatgt ctacaatagt tgatgattgt atagaaaaag
    12361 gcaaggatta ccaaagaggt ggagcaagat acaacactag atatatacaa ggtgttggta
    12421 taggaactat aacagatagt ttaacagcaa taaaatataa tgtatttgat aagaaaaaat
    12481 tcgatatgga tacattgctt aaagcacttg atgctaattt tgaaggatat gaagctatac
    12541 ttaatttagt ttcaaataag actcctaaat atggaaatga tgatgattat gctgatgaaa
    12601 taatgcaaga gatattcaat gcatattaca atgaggtaac tggtagacca actgtttgtg
    12661 gtggagaata cagagtagat atgttaccaa caacttgtca tatatacttt ggagaaatta
    12721 tgggagcaag tccaaatggt agactttgtg caaaaccagt ttcagaggga atttcacctg
    12781 aaaaaggtgg agatactaat ggacctacag cagttataaa atcttgtgct aagatggacc
    12841 acataaaaac tggtggaaca ttattaaatc aaagatttgc accatcagta gttcaaggag
    12901 aaaaagggtt agataatatg gctaatctag taagagcata ctttaatatg gatggtcatc
    12961 atatacaatt taatgtattt gataaaaatg tactgttaga ggctcaaaag aatcctcagg
    13021 attataaaga cttaatagtt agagttgcag gatatagtga ccatttcaat aatttaagta
    13081 gaacactaca agatgagata ataggaagaa ctgagcaaac attctaggtt tgcttgtaaa
    13141 taaaacttct tactatataa aatcgtaact gatataataa agtataaatg gtttgattcc
    13201 atttttaaaa ttttagatta attatgagct aactttctaa ataggaagtt agctcgttaa
    13261 gtttaatgaa aaaatcgtta aatttatatt tagtaaatca tacacatatt agtaagtaat
    13321 acatacaaaa gaataattta ttatagttat aataaggtta tattttataa aaataagata
    13381 attttaagca gttaaaataa agtcaattta gctactttaa aataaaacta atagaaaaaa
    13441 ggagatttga tatgaaaact ttaggattta ttggttcagg gaatatggga agtgcaatga
    13501 taggtggcat agtacagtca ggattagtaa aggcagaaga tataactgta tcagatttaa
    13561 gccaagttgc cttagataaa gtaaaagaag aatttggagt aaatactaca actgattcaa
    13621 aggtagctgt tacaaattct gatgtggtaa tagtagcact taaacctaat atatgcgaaa
    13681 aggctttaac tccacttaaa gaattaatag atgcaaataa aataatagta tctatagcag
    13741 caggtaaaac tatagaaagt ctggaaagtt ttataggttc agataaaaaa atagtaagag
    13801 taatgcctaa tactccagcc cttgttggcg aaggaatgtc agctctttgt aaaaatagta
    13861 atgtaacaga tgaagagtta aatatgatta aggctctgtt tgaatctttt ggtgaggcag
    13921 aaatagtatc agaatattta atggatacag ttacaggtat tagtggttct tctcctgcat
    13981 atgtatttat gtttatagaa gctatggcag atgcagcagt attagctgga atgccaagac
    14041 aacaagctta taaatttgca tctcaagcag taatggggtc agctaagatg gttcttgaaa
    14101 ctggaaaaca tccaggtgaa ttaaaagata tggtttgttc tccagcagga actactatag
    14161 aagctgttaa ggtattagaa gaagaaggct ttagagcagc agttataaaa tctattgttg
    14221 cttgtataga taagtctaaa gatatgagta aataacagat gtaattaagt ttactatatg
    14281 gtttcttgaa ttgttttttt agtgcttatt tatatctata cataaatgta tttggtataa
    14341 gtaaaataag agtaatttac tataaaaagg aatttaatga ttgagtgaga atttattact
    14401 taaatattaa taattaaata gatatatttt taactatatt tgagatatat cattgtgaaa
    14461 aaataaaatt tataagtatt attgtgaggt tataagtaga tttatttaaa aataaattta
    14521 tttatagcct ttttaaattt gaaataatat tcataaggta ataaaagtta caaatatttt
    14581 cttcgctaat aagttttaaa atacctataa tgttttactt tttattataa taaatacaat
    14641 aaatatattc ttatataaat tctatgtcag atatatggaa agataagaac cttgagatta
    14701 tgttaaaaaa tctagatttt atacttataa aaagtttgat tttacaacct tacaaaagag
    14761 aatcaagata ggaatataat tatctttttc ataaatatat aaatgatata ctaatattat
    14821 agtattattg atatttgtat tggaggttga acatgaggga tgaaatttta agttttgtaa
    14881 atgaatgtgt attatttaac agctttgatg aacttaaaaa agaaagtggc ataataacag
    14941 aagatatagt aaaaaagttt aatttaagta gaactcaatc aagtagatta ttaaatgatt
    15001 tagtaaaatc tgataatctt ataaagataa acagtagacc tgttattttt ttacctaaaa
    15061 ataaaataaa tgatgtagta ggacctacaa aaaaaagtat ttatgaaaat ttagatgaac
    15121 ttaaaaatga gtgtaaatta ttaaagcaag agactgtgtt taatagtatc ataggatatg
    15181 ataatagctt aaaagaagta atcgaacaaa taaaaaccgc tgtactttat ccaaatggcg
    15241 gacttacaat aatgctgaca ggtgaaagtg gagtaggaaa aactttttta gccaagataa
    15301 tatacaaata tagtgtaagc agtaatgtat tagaaaaaaa ttctccatac aatgtgttaa
    15361 actgtgcgca gtattataat aatttggagt tattgtctag tctattattt gggcatgtaa
    15421 aaggagcata tactggagca aatgaatcta gagcaggtct aatagaacaa tcagatggtg
    15481 gtgttttatt tttagatgaa gtccatagat taaatagtga aggtcaggaa aagttattta
    15541 catttatgga tacaggtacg ttttctagga ttggagagaa tggagtaatt agaaaagcta
    15601 gggtaagatt aatatttgct acaacagaaa gtctatctga atttttacaa acctttttaa
    15661 gaagaatacc cataaatatt tatgttccca atatagaaga aagagggact attgaaaaaa
    15721 gaaagattgt agaacatttt atttatgaag aaagtaaggt acttggtttg cctatagaga
    15781 taactcaaat tacattaaat gcgattttaa agataaaatt caaggggaat ataggtgaat
    15841 gtaaaaatgt aataaaatat gcatgtggac gagcttatgc aaaaaatcaa agaagaaatg
    15901 gaaatatttt tgttactctt caagaccttc ctagaaatct ttatatagaa aatgtagata
    15961 tatttcaatt ccaaagtggt aaaagcaaaa gcattatctt ttctcctaaa caatttagta
    16021 ttgaaatgtc aaatggaaga aataaaagaa atataatcta tcaaagtttc ataaaatgtt
    16081 tagattactt tgatagacta gaaaaaaatg aaataacaaa ggaaaaattt agtaaaaaga
    16141 gttctaatct aatgattaga ctaatggaca atttagtctt ttcaaataat gatgaaaata
    16201 ataacgtact tatggagctt ataatatcga taatgcagga tatatttaga aatttagaag
    16261 ttaataacaa tataaaatat gatgggaata atgtattggc attttcgtct tatctttatt
    16321 tgaaggacga atcttatcaa ttgtctgatg agtataaagc tttagaaaat aaattactaa
    16381 tatatttaaa aaaagagagt tacaaagaat actctataat aagtaaaata tcaacattaa
    16441 taacgaatag attggatatg tatcttaata atgtagatac aatcataatg attatattta
    16501 ttaaatcaac aattataaga atgaaattta atcaagttaa tgcgactatt atagcacatg
    16561 gatatgctac tgccagtagt atggcgaatg tatgtaatcg gatgttgggt aaaaatatat
    16621 ttacagctat tgatatgcct atagatgcta ctattgctga tgtatcaaaa caaatgtcta
    16681 gatatctaga gggagatagg ataaagaatg gtttaattgt attatttgat atgggctctc
    16741 ttaatcttat atatgaagat ttaaagaata agataaatag cccaatgtta tttatagacc
    16801 aaatatccac tatttctgca ctagaagtag gaaatttatt aatgcaaggt aaaaacattg
    16861 aagagattgc agaaattatg gatgaagaaa ctaaacctaa tatacaattg tattatcctc
    16921 aaaaaaataa agaatatgct ataatatcat cttgttttac agggataggt attgctataa
    16981 aaatccaaca tctactttcc gaaagtttaa ttggaatagt tgatgtcaaa ataatacctt
    17041 atgattatga tagtcttgtt tctatgggag ataaagaacc agtttttgaa atgtatgatg
    17101 tgatggctat agtaggaact gccaatccaa atgtgaatgg ggtagatttt atattattag
    17161 aggatataat atcaggaaaa ggagaagatg atgtttttag aatatttagc agagtagtag
    17221 aaaatgaaaa aataaagaac ataaatgatt ctattgtaaa gaatttttca ttaattagag
    17281 taatagatag tctaactatt ttagattcaa aaaagataat agagagaata gaagaaggaa
    17341 ttaatgctat agaaaatatt caaaagaaaa aactttcaaa tgataaaaag atttctttat
    17401 atgttcactt aagttgtatg attgaaagat tagttagaca aactgaaatt gaagagtata
    17461 ctgatatgga tttgttaatc aaaaaccatc atagtgaagt aaaactaata aaaaatgcat
    17521 ttagtgtgct agaaaagagt tatagtgtgc aaataccaat ttcagagata ggatatattt
    17581 ataatattat ctatggtcta ccacagtaaa atacaagatt acaaaataag tgaatgaaat
    17641 ttaaaaaagt atgccagtat ttggcatact ttttgctttt atagataaat gagagtataa
    17701 acaataaaaa ttttaatgta tgatggagga tttggggaaa atggtatctt tgatgagaat
    17761 tgatgacaga ttgatacatg gtcaggttgc atatggatgg gcaacatcac taggtataaa
    17821 tgtaatacta gttgcaaatg atgaagcaaa aaatgaccaa atgaaagcta tgtcactgaa
    17881 cttagcgaaa cctagcaatg taactttata tataaggggg gtagaggaat ctggagaaat
    17941 agtagaaaaa tttgcttctt caaagaaaag caatgtatta gtgttagtaa aaaataccaa
    18001 agatgcttta gttgtagcta aaaattcagg aggagtatta aaagaaataa atgtaggagg
    18061 attaagatat gaggaaggga agaagaaact tacagattta gttgctgttg atgatgatga
    18121 tataaataac tttaaacaaa taggtgattt agggattgat atagaattta gaatgttacc
    18181 tagagacaaa aagaaacaac tatcagattt aataaaataa tttaggagaa agagaatgaa
    18241 aaaatttcta attgcaacac atggagaatt gtccagagag cttgttgaca caagtaaatt
    18301 aataattggt tcattaacta atattgagta tttttgtatg acaacggata aatcagcaga
    18361 tgatgctgag aaagaaatta gaagtatatt atcaaataat aaagatagtg aagaatttgt
    18421 tgtcttaaca gatgtgtttg gtggaagtgt ggcaaacata tgtacaaact tacttttaca
    18481 agggttcaaa tttgaattat tgacaggagt aaatttacca atgcttttat ctatactttt
    18541 attagttgag gaggatacaa aactaatagt taaatcagga ttagaggaag caaaaaatgg
    18601 aattgtttat gtaaatgagt tattagaaaa tcaaaacaaa atcaaaaatg taatgtaatt
    18661 taatgtaaat taaactaagt taaaatggag gttcaatatg ttagtacaag ctactatgtt
    18721 agctattata gcaggagttg gtatactaga tgggcgtata tttggtcaat ctatgcttga
    18781 taggccaata gttacaggta tgttagtagg attagtttta ggagacataa aatcaggaat
    18841 tatgattggt gcacagcttg aattaatttg gatgggaatt gcaggaatag gtgcagctac
    18901 tcctccagat gtagtaacag gaggtgtatt aggtacagct tttgctatat tatcaggtaa
    18961 tggtgcagaa gttgctttag cagtagcagt accagtagca gtcttagcac aatctcttgg
    19021 agtattagtt agaataataa attcgtactt ttctcaaaag gcagttttct atgctaaaca
    19081 ggctgatttt aagaaagtta ccataatgat gtggttgcca gtaatattat tctttttaag
    19141 tacatcagtt ccaacctttt tagcaattat gattggtgct gataaagtta cagcattaat
    19201 aaatgcaata cctaaagtta tattagacgg attgggtgtt gcaggaacat tgttgccagc
    19261 agtaggtttt gcattgctta tggatatgtt actttcaaaa aagatgtctg tatttttctt
    19321 tatagggttt ttgttagcat cttatggtgg attagatata actgctatag ctttatttgg
    19381 agtatgtgta gcatttgtac taaactttta tatcaataac aaagaaaata atcaaaatcc
    19441 tcaattagca atcaatactt taacggaagg agagattgac tttgaataaa ttaacaaaaa
    19501 aagaattaag aagtatgttt tggcgttcat ttgctcttca aggtgcattt aattacgaaa
    19561 gaatgcaaaa cttaggttac tgttattcaa tgttacctgc aataaagaag ctatacaata
    19621 agaaagaaaa tcaagcaaaa gcaatagaaa ggcatttgga gatttttaat acaacgacag
    19681 tggtagttcc tgctatatta ggtataacag ccgctatgga agaagagaat gctaataatc
    19741 cagaatttga tgaaagttca attagtgctg ttaaaacagc tcttatggga cctcttgcag
    19801 gaattggtga ttcattattc tggggaacat ttaggattat agcagcagga gtaggagtat
    19861 cacttgcaaa agaaggtaat atctttggac cactattatt cttactatta tacaatatac
    19921 cagcatttgc ccttagaata tttggactta agtatggtta tcaagtaggg gtaaattctc
    19981 ttgaaagaat acaaagagag ggacttatgg agaaaataat gtctatggct acaactgttg
    20041 gtttattcgt tgtaggagga atggttgcaa ctatgttgag tataactact cctctgaaat
    20101 tcaacttaaa tggtgcagaa gtaattttac aagatatact agataaaata atacctaata
    20161 tgttaccttt aacctttgca tttgtaatat actacatgtt aaagagaaag gtaagtgtaa
    20221 caaaattaac tataggcact atagtaacag gaatcgcttt acatgctata ggtttgttat
    20281 agaaattaat tataatttat tgaatatata gataaggaag gtgtataaaa tgacaataca
    20341 agattatatg ttagaaacac cagttagaat gagagaaatt atatctaatg cagatagctt
    20401 atttaatgaa gttaagagaa caaatctaaa aaagattata ataacaggtt caggtactag
    20461 ctatcactca ggagtgcagg tgcaaccata tttacaaaat ttattagata tagatgttgt
    20521 gaaaatgtat ccatttatga taactgaaga tacattcaag tttgataatg aaaataccct
    20581 agtagtaggt gtatctcaag gtgggagtag ttattctaca tacaacgcta tgaagttagc
    20641 tgaagataag ggctgtaaaa tagcatctat ggcaggttgt aagaatgctt taatagatga
    20701 gatttctgat tatatattaa cagtaaattg tggtgaagaa aaatctggag ctaaaacaaa
    20761 aggatattat tgtacaaagt taaacttaat gttattaggt cttcaaatag ctagagaaaa
    20821 aggtataata tctagtgaaa agtacaatga ggaaataaat aagatattag acgcaattaa
    20881 tagatttgaa gcagtgtata agctctctaa acaatggatt gaaagaaaca aagaaaagtt
    20941 agttaattct aaagaaataa gaataatagg tcactctgat atatatggag atacattaga
    21001 agcagcatta aaattattgg aaacaatgag aatacctgta actgggtatg agtttgaaga
    21061 gtttatacat ggtatatata atgctataaa tagtgattct actatattta tactagatac
    21121 aggaaaagaa cctagagtaa ctaagatgat tgatgtactt tctggttgga cggaaaatgt
    21181 atttgctata ggtagagatg ttacagaaaa tgataaaaat ttaaagattg atatcacaga
    21241 taatccttac tatcaaacat ttaattttat agtacctata caactaatat gtggggaaat
    21301 acctacatta agaggtgtag acccaagtgt tccaaaagat actaggttcc acatgaagtt
    21361 aggtagcaag aaactgaata agtaacaaaa aaagaggttg tctcaaaata gagataagcc
    21421 tcttttttac tagataagct cttttttact gttgaaatga tgaaaatata tttgatttta
    21481 tataaaaaag agtgcctcat gaactaaaaa gttaattttg agacacccac ttttatctta
    21541 ttttaaacta aaatatttat atattagtgt gcattatttt ttttattgct gtcttcctta
    21601 cttgagcaag aattagcact tggacatcca gaacaaatag aacatccact acagacattt
    21661 ccgttttttg tagacttgaa tttattaaac agaataaaga gtgcaaggac tataattatt
    21721 ccacctatca aagcttccat tattgaatta cccataaata gtactccacc tatactttta
    21781 actataaaag ccattatcca tgctagtata aattgatatg caaatgatgt aaacatcatt
    21841 ttattgccat attctttttt catagttgca agtgcagcta tacatggagt atataaagca
    21901 gtaaatatca agaatgtata tgcagtaact ccattaaata ttgttggtaa gacaacagtt
    21961 agattaccat atagtatatt taaagagttt actacgattt cttttgctcc aagaccactt
    22021 aaaatagcaa ctgaagttct ccaatcatca aaaccaagag gtttgaatat aggagatata
    22081 atatgaccta gagtagccaa gaagcttaca tttatatctt ctgtatatcc aaaaacattg
    22141 aatgaagaaa gtccccaaat tacgactgac attgcaaaca tgacagtaac aactcttatc
    22201 aagaaacctt ttgatttatt ccaagtattt ttaataagtg catctatagt agggattcta
    22261 tattctggta attctaatat aaatggttca gcctctgttt taaaggctgt tttatttaag
    22321 aataatgcaa ctacaattgc cataactata cccaccaaat acagtgatgt agttactaaa
    22381 gcagcattat gtggaaagaa tatagctaca aatagtgcat atataggtag ctttgctcca
    22441 caagtcataa gtggtgcaat aagagctgta actcttctat ctttttcgct ttccaatgtt
    22501 ctagttgcca ttatagctgg agaagaacat cccataccca taactatagg tataaaagct
    22561 ttaccagata gaccaacctt tctcataact ttatccatta aaaatgctgt tcttgacatg
    22621 tatccactat cttcaaacaa cgatatacca aagaataatg taaatattaa agggaagaat
    22681 ggtaaagtac caccaacacc tccaattatt ccatcaacta taagtgattt aaaccatggg
    22741 ctagagttag ataacatatc actaacaggt gcagaaatat aagcctctat aagactagca
    22801 aatccctctt gtagtggtcc tccgacccag tcaaaggtaa atttaaataa gaggaacaat
    22861 acaccaataa atatcggata tgctaatata ggattcaaga ctatattgtc tatcttatca
    22921 ctgatagatt tcttttgtgt atatgtagtt tttgtacatc tcgataaaat ggattctaat
    22981 tttttatatg tttctgtttc acttccaaag tttttctgat atataaaagc tttttgcgta
    23041 gctcccttaa tctcttcttc tatcctatca acaccatttt tcttttttgc tataattgga
    23101 acaacaatta ctccaagttc ttcacttaat tttttggcat ctatattaac tcctttagat
    23161 tctgcaatgt ctaacatatt aagcaatata actataggct tgttaaattg cataagttga
    23221 gttgttaagt aaaggtttct tgataaatta gaagcatcaa ctacatttac tattacatca
    23281 acatcttcat tttctaaata tgatttagaa accttttctt catttgagaa agtatccatt
    23341 gcatatatac caggtaaatc gacaaccttt atctcttttc caagaaatcc ttcttttttt
    23401 tctatagtaa caccaggcca gttacctaca tattgatttg aacctgtaag tagattaaaa
    23461 acagtagttt taccgacatt tgggtttcct aataatgcta cattaatcac gggtagctcc
    23521 tcctctatta ttgtaaagaa atatttttag catcagattt tctaatcgca aggtcaaagc
    23581 ctctaaatct aattataatt gggtctccaa gtggggcaac ctttttaact tctatttcag
    23641 tactatttat acatccaagt gcaagcaatc ttttagataa tttaatatct ccatctatac
    23701 tattaataaa tcctttttct cctattttta aatcgtaaac agtcattatt aacacctcct
    23761 aatgaaattc attatcatta aaattgtaac aagtatgata caaaaagtca agatttatag
    23821 aggtaattgc acattgaaat aactaatatt tattctaaat agattaaaaa tattttagtc
    23881 taaattaggt gtttttatat attttgtatt ataatgtaag atgatagata aaatttggag
    23941 gagttaactt aatgaaaact agaaaaatga catttttagg tcttatgata gggtatagtt
    24001 tagctttata tgtattagag acatatatac caaatcctct tatagttata tttccagggg
    24061 caaaattggg tttgagcaat attataactt taatttcatt aatgttgtta ggtgtcaaag
    24121 atactgttat aattgttact atcagggtaa ttttgtcatc aatatttgca ggccctttat
    24181 catatctact atttagtata ggaggagctt atctgagtct tttatttatg tatttagcga
    24241 gtaaaataaa agatttatca ctaataggga taagcattat aggtgcaata gggcataata
    24301 taggtcaatt aatagttgca agtttaatca tagaaaatat acttgctata gggtacttac
    24361 catttatgtt aatggcatca ttagtaactg gtatgtttgt aggcttagta tccaggtata
    24421 caatacctaa attaaagcat ttttatagta agttttattg acataatatt gacaaaatat
    24481 aatattgttt gataaaatgt atataaaaat atacaaattt gtaatagtgg tgattattat
    24541 ggcgaacaat gaattagaag cgttaaaaaa tgagattgaa gcagttaggg aagagataaa
    24601 tacatacata gaatatccag aaatatttca agaagagtta gtagaatcaa gtaagaaaat
    24661 agatatatta ataaataaat acatgtacct aagcaaatag cccacagttg tgggtttttt
    24721 tgtatcaaaa attaacagat ggctatattt tacatatatt aataataata ttaagatata
    24781 aaatagtatt tctgatgtgt aaattatctg taggacataa gttgtaatta agtcaggagg
    24841 gttttatatt atgattgata taggaagtaa ggagatagca ataaggttac tattagcact
    24901 aataatagga ggaataatag gcttagagag agaaaaaata agacagtttg caggatttag
    24961 gacacatata ctaattgcaa taggttcttg tattgtttca ataacttcga ttcaattatt
    25021 ctttgattat aatttgtata caaatgctga cccagcaaga atgcctgctc aagtattatc
    25081 tggaattggt tttttaggtg caggagcaat tttaaaaaat aaaggtgggg taaaagggct
    25141 tactacagct gctgggatgt gggcaactgc atgtattggt atagcagttg gatatggtta
    25201 ttatgaattg gcaattctag gtggattatt tgtaatgata gcattatttg tgcttaggat
    25261 aatcaatacg tttttattta gccaagaaaa gaatgtattg atattaaaat taagcaattt
    25321 ggactctata ccttttttat atgataaatt tgaaaaaaat caacttatcg taagaaatat
    25381 ggaaatagaa tgtcgtaatg aagaatactg gaaggttaat ttttttgtat cttatgctaa
    25441 gagaatacac ttacacgatg ttatgaaaga aataaatatg atagaaggag taataaatgt
    25501 agattatttg gactaaaata tatacaatat attttgtaaa gtgaataaaa tataaaacaa
    25561 attaaaatag aggtgtatga tatgggaact gatagaagta caatagaaga aaagtacaaa
    25621 tggaagatag ataagatgta ctccagtaaa gaggaaattg aaaaagacat aagtaaagta
    25681 aaaaatctta tccaagaagt aaaagaatat aaagggaagc tttctgagag taaggagaac
    25741 ttatataaag tattaaatat ctctgaaaat gcatctagga ttgttcaaaa tttatatgta
    25801 tatactcata tgaaacaaca tgaagatact agaattaatg ataatcaagg aagagccact
    25861 aagacagaaa tgttatctac tgatttaggt gtggctactt cttatatagt tccagaaata
    25921 ctggcgatag aagagaataa gttaagtgaa tacttaaaag atgagaagct atccttttat
    25981 acaaaatata taaaggatat attgagagat aaaccacata ctttgagtga acgagaggaa
    26041 gaaatacttg cagctacatc agaattatcg acaattccag aaaatgtata cgatatgtta
    26101 gcatatgcag atatggaatt tcctgagata gaagatgagg aggggaataa ggttaagctt
    26161 tctcattcaa actactcttt atttataaaa agtaagaata gtagagttag aaaagacgct
    26221 tttgaaggtg aattttcaac atatgagaaa tataaaaata catttgcctc aacgctttat
    26281 ggtggaataa aatcagagat attttacgca aaaactagaa agtataattc agcaatagaa
    26341 gcgtctttat tttcagatga tgtaagttta gatgtttaca ataatcttat atcagctata
    26401 gataaaagta ttcctaatct aaataaatat gttgagctta aaaagaaatt tttaggattg
    26461 aatgaaatac acatgtatga tttgtatgtt cctttgacag ataaatttga catggatata
    26521 ccatatgata aagcacagga tatcatatta gaggcactta aacctttagg agaagaatac
    26581 ttgaaggtca taaaaagtgc ttttgatgaa gggtggattg atgtatatga taatgaaggt
    26641 aaaaaaggag gagcttattc ttggggaagt tatgattcac atccttatat attaatgagc
    26701 tataaaaatg accttaattc tctattcact ttagttcatg aattagggca ctctgttcac
    26761 agtcattatt caagaacaag tcaaccattt ttatattcgg actataagat attcgttgca
    26821 gaagttgcat ctacattaaa tgaattactt cttataaatt atttattaga aaattctaag
    26881 tctaaggatg aaactatata cttattaaat tattatttag aacaatttag aactacagtt
    26941 catagacaaa ctatgtttgc tgaatttgaa aaaattgtac atcaaagggt agagagtgga
    27001 gaaccactta ctgctgatga atttactaat atatactaca aattaaatga aaagtattac
    27061 ggaaaatctg caatagtaga taaacaaata ggaatagaat gggcgagaat acctcatttt
    27121 tattcaaact tctatgtata taagtatgcc acagggtttt ctgctgcaag tgctttaagt
    27181 caacagatac tttcagaagg aagtactgca gtagatagat atataaactt cttaaaaagt
    27241 ggagggtcag aatacccact agaacaatta aaatctgctg gtgtagatat gacgaaaaaa
    27301 gaatctatag aggaagcctt aaatgtattt gctgaattag ttaataaact agaaaaagaa
    27361 ttataggatg tgataggttg atagcttcaa tgaagacttc tgataatatg agttgtatta
    27421 tgtttatatc tgcctaaagt aactttaaaa agttaatcta attaagaata aatcatataa
    27481 aattaagttt ttacatgtat ttaacaatat ttacatttac ttaaccctgc tctaatattc
    27541 atttaacaaa gaatctgtat tattaaaata tagaaaaatg acaacaaaaa attaaaatat
    27601 aagcgggggt aagtaaaatg tttaaaaaga aaataggcgt tcttttagct agtacaatgt
    27661 tagttggagc tttagcagta ggatgtagtt ctaactcagg aggttcaggt gattcaggag
    27721 attcaagcaa agtttctgtt tcaggttcaa cttcaatagg tcctcttatg gaaactatcg
    27781 gagaaaagtt tcaagaaaaa aatcaaggtg ttagtgtaga agtacaacag attggttctt
    27841 ctggaggtat aaaaaatgct atagaaggaa catctgaaat aggaatgagt tcaagagatt
    27901 taaaagatga agaaaaaagt gctggattaa aggaaactaa aatagcaata gatggtatag
    27961 cattgataac taatactgca aactcagtaa aaaatctgac aatggaccaa gttaaaggta
    28021 tatatacagg aaagattact aactggaaag aagtaggagg aaatgatgca ccaatagtaa
    28081 tagtatcaag agaagaaggt tcaggaacta gagatggatt ccaagaaatt gtagggtttg
    28141 attcagaaga actaactaaa gatgcacaaa taagtgatgg ttcaggaaat ataaaaacta
    28201 cagttgaagg aaatgaaaat gctataggat atatatcatt tggatatgtt gatgagaaag
    28261 taaatagttt aaaaataaat gatgttgaag caactgatgc aaatgtaata gctaataaat
    28321 atccaatatc aagaccattt atattagtta ataaagaaga tggtttgtca gaaaaaggta
    28381 aagctttaat agattatata ttaagtgatg aaggacaagc acttgtttca gaaaaaggat
    28441 ttattaaagt taatcaataa caagagttat aatataaagt aaacatataa taccctgtga
    28501 gataagctca taaaaatgat gctttaactt gcagggtatt gtctattatt gaatgtagaa
    28561 atataaatat ctttagataa aataggtatt ttagtatctt atctctaaga atatggaaat
    28621 attgacaaaa taatattaca atgttagaat aaaaaagaaa tttatgaaaa tatataaaag
    28681 atttttaata aaaatatttt agaaactgag aagtatatct tattattggg catctagaga
    28741 tatatggagt tagtggtgca accggctatg aatataattt ttatattgca tagtcttttt
    28801 ttgttttaaa attaacaaaa atgttgcaaa atttgaaaat aagagtatta gtcgttaaga
    28861 tttttattga taggtaaaat tttggctttt aaagtagcca tttgttggaa aagggaaatt
    28921 tttttaaaag aataatagta ttataataag aaaataaagt ttttataaac attgagatgc
    28981 agaatataaa ccttttgtaa caatatattt accaaaataa tacaatttat tttatattat
    29041 aaccatataa atcatataaa tcaatactca aactagccaa tagacaagtt tctagaaaaa
    29101 taataaattt atacgtgaaa atgactttaa gctccattct ccaacaatcg gagactttaa
    29161 ttagtattta ataatgaatc ttaatgttta atacaatatt caagaattat tcaaaaatac
    29221 ttaagtatta tagtctagtt tgtaaagata ttataaaata ataataccag ttatatatga
    29281 aaataaaaca ttataatttg taataactat aaaacttgga attataatta aaataccttt
    29341 gaatggagtg gtgtaatgta taatttactg gttgtagatg atgatgttga tattttaaac
    29401 ataaatcaaa tttatttttc taatataggt tttaatgtat atacatcaga gactgctaaa
    29461 gatgctatgg atatagtaga gagtatagtt ttagactgta taattttgga tatttcattg
    29521 ccagatatag atggatataa aatgtgcaat gatataaaag aaaaggttaa catacctata
    29581 atatttttat ctaattatga gcatgaagaa gaaaaagttc ggggattttt agctgggggg
    29641 gatgattata taacaaaacc ttatagttta aaagaacttg aacttagaat atatgctcgt
    29701 atgaggcagt acaaaaatat tacatcagtt cctaatgtgt taaagttttc agcgttagta
    29761 ataaatgtaa attcaagaaa agtaactttt gatggagaaa gtatagattt aactactatg
    29821 gaatttgaaa tattattgtt tctagctgag aacaaagaac aagtattttc aaagatagag
    29881 atatataacc aagtttggaa aatgcctgat attggagatc aacatacagt gcaggttcat
    29941 attgcacaga tgagaaaaaa aattaattct ttaagcaggg agcatcaata cattcaaacc
    30001 gtatggggaa aaggatataa gtttgtacca taagtgagat actgtaatta ataaatagtt
    30061 cttgttttat ttaatactac tatgaaagag atgtggttga tacaatgaaa aagtataata
    30121 tactaaaagg aactctcatt gtattgatag tctctctgct ggcaataaca ggattaaatc
    30181 ttctgtataa atatgataat aaatatacat ataagtctat tagagcaagt aatggaattt
    30241 taactataga taatgatact ttcactaaag gtaaacttgt ctttttgata gatgactggg
    30301 agttttataa tcataaaata tttaaaccga atgattttac agagcaagat ataaaaccag
    30361 aatatgtttt tataggtcaa tatccagatt tttctatgag aaaagggact cagtcaccat
    30421 atgggaatgc tacttatcgt atgagaataa aaaatgaagg caaggataag ctactaagtt
    30481 tagagcttcc tgagattttt tccgcaagtg aagtatggat aaatggagaa atggtttcta
    30541 aattaggaga tgtaggaaca gaaaaatata gacccaaaat taaaaattca gtagtaagtt
    30601 ttattgcaaa gaaaaataca gaaattataa tcaatgtatc gaatttttca cattattata
    30661 gtggtttgta ttatccgcct gcattaggag aaacaaagga cataagtaat atgatgtttt
    30721 atcgattact tttttatagt ataatttgct tcacaacttt tgcaatagct atattttcat
    30781 tgtctgtatg gtttctttca aacaaaagta aaatttactt atattttgga tgtatgtgtg
    30841 ttttctttgc catccatgta tcctatccct ttgtacattt gttaggactg cctttggtaa
    30901 gctttacata tgcggttgaa gatttgtcat attttatggt agttttatgt attatttgta
    30961 taaatggaag tatatccaaa atagaagaaa ataaagtgta tagattttta attttaccat
    31021 taggaattac aatgagtatc atatctatag tgataccaat tattcttctt ccatatgata
    31081 tgtactttgt caacatatat gggaagttaa tagacatata taaatatatt gtatttatat
    31141 atatattaat gtcttcgctg gtttcaatat ataaaaagac atgctctgag catataagtt
    31201 cttatatttt agtttcagta aacactgttt ttggaattag catattattt gatgcagtta
    31261 cgagtaatag atttgagcct atatttacag ggtggcaaac agaatattgt gggtttgtaa
    31321 ttgttatttt attttctgtt atgatgataa acaggaatag aataataata aaagagaatg
    31381 aaaaacttac aaggaatctt gaacatgaag ttgaagtgag aactaatgaa cttacaacat
    31441 tgttaaatga acgtaaaaga ttcttggcag atgtagcaca tgatttaaag gcacctgttt
    31501 cggctataca agcatttata gatttgataa aggttggtaa tattcatgta gatgaagaga
    31561 caagaaccta tctttatgct ataaatcaaa agtcaaatga agtacaaaat agagtacgta
    31621 gtttgcaaga atttacatca caagataaaa gcattggtat tagtgaaaaa atatgtctga
    31681 ataattttat taaagagata tatgaaaata atttgccaga tacacaagca tgtggtgtaa
    31741 actttgaaac ttatgtattg gagaaaaaaa tatatgcaaa gataaataaa gagatgctta
    31801 caagagcatt tgagaattta atttataatg ctttgacctt tactccattt gatggaaaga
    31861 taattttatc aatggataaa gaagatggat ttgcaattat taaaatttct gataatggaa
    31921 aaggtatacc tccagaaaat ataccaaagg tatttgacag attctttaca gaaagagaca
    31981 ataatgagcc aaaagggcaa gggttagggc tatatattgt caaatccatt ataagagagc
    32041 atggtggaga aatatttgta gattcagaac taggaaaagg aactgttttt acaataaaat
    32101 taagtctatg ttttttataa taatagctta tttaatataa aagaataatg atatttggga
    32161 gtgattataa tgcaaactaa gatattgata atagatggag acaaggataa ttgccagaag
    32221 ctaaaaggat ttctggagga aaaaggaata agtatagatt tagcatataa ttgtgaagaa
    32281 gctattggaa aaatatttag caataaatat gatttaatat ttttagaaat aattctaagt
    32341 gatggtgatg gatggactct ttgtaaaaaa attagaaatg taactacttg tccaattgtt
    32401 tatatgactt atataaatga agaccaaagc attttaaatg ctttaaattc tggtggagac
    32461 gattacttaa taaagcctct aaatttagaa atattatacg caaaagtaaa agctatttta
    32521 agaagaatga actcatatgt aaataataat gaaaataata caaaattaga ggaatataat
    32581 agaactagtg gagtaataaa gttagaagat aaaataataa aattgactcc aacagaaaat
    32641 aagttgttaa acttttttat agagaatcct gaaaagactt tgactactaa agagatatat
    32701 gaacatgtat ggatgaatga atacttagag gataactata gtgtggtagt tgcagtgaat
    32761 ggattaagaa agaaaataga gaaagattat aaaaatcctc agaaaattat aacaataaga
    32821 ggagcaggat attattttaa taaggaataa taaatatatt aatatcatta ataattgtta
    32881 taaatgtgaa atactaacga tatctaaact aaattattat aaaatagctg actgtaattt
    32941 aattgataaa tcctattctt ctagactttg ccagttaaat tacagtcagt tttattatat
    33001 aaaaaaatgc gtaatggaac tattatttta tagattatat ttgaaaagat ggataaattg
    33061 tatatctaaa tctatatatt attttattat ttaatatata ttaaataata aaatagttaa
    33121 ataataagat taagaatttc ttaataaatc cagaagatgc tttcgattat ataatatttt
    33181 atatttttat attattaatt atatgtgttg caatcgctgc acctactttt tctggtgagt
    33241 atcagacaca atcagatagc atccttcgtt gtacgaaaca tggacatatt cgtctagcgt
    33301 ttactaaaat attagctcta ttttctatat ttataataat gtttgttata tgtatttcta
    33361 tccatttggc aatttctgat ttagcttttg gtactgaatg tttgaaaacc tcatttcaaa
    33421 tgttgttttc tgtcattagt ctagtaaata taaatttact acaagcacag attcttatag
    33481 ctattggtgg tctaatttca atacttgcaa tggttagttt aacattattt ttatctgcaa
    33541 aatgtaagga ttctcttact tctatgctga ttgctttttc tatttgctta attcctatgg
    33601 gaataggtgt tagttggatt ggatacctat taccaccagg gggaattggt tttaagatta
    33661 gtttattata tcaaatgaca tcatttaatt tcttacactt aggaggtatg agtttttgga
    33721 caccacacat tatactgttt ttctcagtga tagaaatacc tatttttata tttttggcta
    33781 ttcgtaccta ttgcaaacat caagtaccgt aacaatttga tggaaataat agtgatataa
    33841 actagaggat acaataatta tatatgtagt ataaaagtta aatttagtta acaaatctta
    33901 atagcagata taggtaataa attatacata aactgtatta ttatctatat ctgctatttt
    33961 aatatagaga aacaacagta tattgtaaag taaatgtcaa atatagtttt aaaagtaaaa
    34021 ttaatgttaa aaagtaaata taattttaca cttgcttaac ttaatcctaa tatagattta
    34081 acttaaaaaa agtagaataa aagtatagga aatttgatga agacgaatta taaaaaatta
    34141 aattttgaat acagaacgaa aatcgataag tatattttaa tgggagggta ttatgatagt
    34201 aaaatctaga ttagtaggtt tagaaaaaaa tgacaataat ggcaataaga caaaatacgt
    34261 aattgaaaaa gtagcaaaaa atatttttct acttagtgct ttagtagcag ttgcaagttt
    34321 acttttgata ataggattcg tattttataa aggattaaga ccatttatat ttgaaggata
    34381 ttcatttata gactttttaa ctggtgcaga ttgggttcct tctgccaata aatttggaat
    34441 atcatctatg atagtagcat caatatttgc aacacttgga gcacttgtta taggagtacc
    34501 tgttggtata ttaacctctg tatttatagc agaagtagca cctaaaagat tagcaaaaat
    34561 aatgtcacca gcagttgaac ttttagcagg tataccttca gtattatatg gtgtatttgg
    34621 tcttgcagtg atagttccaa ctatacaagg tatatttaat cttccaaaag gtcaaagttt
    34681 gttagcaata gtaatagtat tagcagtaat gatgttacca acaataatat ctgtatcaga
    34741 aacagcaata agagcagtac caaaagcata caaagaaggt tcattagcac ttggtgcatc
    34801 aaaaatagaa acaatattca aagttgtatt acctgctgca aaatctggag tattagccgc
    34861 agtagtgtta ggagtaggaa gagctttagg tgaaacaatg gcagtaatac ttgttgcagg
    34921 caactcacca gttatgccaa ctgcaataac agatagtgta agaccactta caactaatat
    34981 agcacttgaa atgggatacg catttggtac acatcaagaa atgctatttg caactggagt
    35041 agtattgttt acatttatat taattttaaa cttagtttta aataaacttt caaataaggc
    35101 ggtaaattaa gatgagaaag ttcaaagaga atctattgaa gtcattagta tatctatcag
    35161 cactatttac aatagtatca ttagtagtta tagtgggatt tatatttata aagggaattg
    35221 gcaacatgaa ttttaatttc ttatttagca attattcagc atcaggtgat ggtggtatac
    35281 ttccaatgat aattacaaca ttatatacag tagtagtttc tatagttgta gcaactccta
    35341 taggaatatt ggctgctata tatttacaag aatatgctaa aaaaggaaaa gcagttacag
    35401 ctataagatt tgcaactgaa agtttatcag ggataccatc tatagtatat ggattatttg
    35461 gaggaatttt ctttgtagtt acattaaaaa tgggatattc catagttgca ggttcactta
    35521 cagttgcaat aataatatta ccagtaataa ttcgtacaac agaagaagct ttaaaaacag
    35581 ttcctcaagc ttatagagaa tcatcattgg cattaggagc tacaaaattt caaactttat
    35641 ataaggtagt acttccaagt gcaataccag gaattttatc tggagtaatc ttatcagttg
    35701 gtcgtataat tggagaatca gctgcaatat tacttacagc aggtactgta gccaagatgc
    35761 caggtggaat atttgatagt gcaagaacat taactgtaca ctcatactta ctgactaaag
    35821 agagtggaga tatagcaaca gcagccagta taggaattgt tttaatagta attgtattag
    35881 cactaaacat gctagcaaga tttgttgcta agaaattaaa taaagctaat tattaaattt
    35941 aagaggtgtg aataatggaa ttaatcgata agataaaaat gagtgtaaaa gatttggatt
    36001 tattttatgg tgataagcaa gcattaaaga aaataaatat ggatataaaa gagaataagg
    36061 ttactgcttt aataggtcca tctggatgcg gaaaatcaac ttttataaga acgttaaata
    36121 gaatgaatga cttaatcgaa gatgtaacaa ttaaaggaaa catttctgtt gatggagagg
    36181 acatatatac ctcagatgat gttataaatc ttcgtactaa agtaggaatg gtatttcaaa
    36241 agccaaatcc atttcctatg agtatatatg ataatgtggc atatggtcct cgtacacatg
    36301 gactaagaga taagaagcaa ttagataaaa ttgtagaaga aagcttaaaa ggtgcagcta
    36361 tttgggatga ggttaaagac aggcttaaat catctgcact aggactttct ggtggtcaac
    36421 aacagcgtat atgtatagca agagcaattg ctatgagacc agaagttatt cttatggatg
    36481 aacctacatc tgctttagac ccaatttcaa cattaaaagt agaggaactt atagaggatt
    36541 taaaaaaaga ctacacaata gtcatagtta cacataatat gcaacaagct gctcgtatat
    36601 cagatgaaac agcttttttc ttaaatggag aggttataga atttagcgat acaaaaacta
    36661 tgtttaccac tcctgtagat aaaagaacag aagactatat aacaggtaga tttggttaaa
    36721 atatataact ataaaggagt tagacaatgg tcaataatag tttagatata agtattgata
    36781 ctttaaaaga acaaacagtg cttatgatgg aaaaatgtga agaagcaata gaaaagtctg
    36841 tgacttgtat gatagagaag gatttagagg gtgcaagaaa agtagtagaa gatgatgaag
    36901 aaataaataa attaagagaa gaaataagag ataagagtat agagctaata gtattaaagc
    36961 aaccaatggc aagggactta agatttattt attcattagg taatatatct atagagctgg
    37021 aaagaatagg agattatgca gcaaatatag ctagagaaat gttgaagata ggtggagagg
    37081 cttatataaa agagctaata gatattccta agatggcaaa agaatgtaag tctatgatgt
    37141 ctcagactag agaagcttta atcttatttg atgcaagtat agcttatgat gttggaacta
    37201 aagatgatat tgtagatgga ttgtatgaac aagtacaaat tgattgttta aaaataatgc
    37261 atgaaaactc aaataccata gaccaagggg taaaacttct acttatagga agataccttg
    37321 aaagaattgg agaccatata acaaatgtat gtgagaagat aatttttgca attgagggta
    37381 aaatggttga gataggataa aaaactgctt taaagcagtt tttttattgt ctaaattaag
    37441 aaagaattga taaaaaaata tgattaatgg gtaaatttaa aaaattgtga tatactagga
    37501 acatattaaa tatgaggatg cgtattttat ataaggtgct ttttaggagg tggaactttg
    37561 aaacttcaaa agatgggtat gaggactata aaaacaggaa ttagtgtcac tttatgtatg
    37621 attcttggtg actatttagt agagaatatg ttttattctc tggtagcatg tataatttct
    37681 gttcaagata cagtaaaagg ttctttaaag acgggaatca atagagtgga aggtactatt
    37741 ttaggtggta taataggatt catatttgca atcattaaag ctggagaccc atttttatgt
    37801 ggtctaggag taatgtgctc aatatattta tgcaatctgt ttaaaataaa gtcagtagta
    37861 gttgcctgtg taacctttct ggctatacat ctaggtgtag gaaatagtga ccctgtgtat
    37921 tattctttac atagggtgtt agatacatct gtgggtgttt tgtttggagt acttgttaat
    37981 tattatgttg ctagaccaaa ttatctaaat agtgttatag atgagtttaa aaagatagaa
    38041 gaactttcaa tgttgcttat tgaagataaa attcttagaa aagaaaattt aaatgtagag
    38101 aaatttgaag aagaagtaga aaaattagaa gaaatatatt caaaacttat tgatgagttg
    38161 gattatagtt tgagtgaaac taatatagaa aaaattgaga atgccttaag gatatgtaga
    38221 gaaatacatt ttcatatgca gtctatagag ttacttgaaa ataaacttta tttgaaagag
    38281 catacatata aataccttaa gaaaacatat aatgtggata agctagactg gaatatagat
    38341 gaagaaaaaa gccctgtatt caactatcat ctaatgaaaa taatgattga aatagagttg
    38401 ttgagaaagc taaataaatc atattaataa tagtagttaa ataataaata aaaaatagta
    38461 tctttgtaaa tattaaacat atataagttt aatttatgga ggtactattt tttatgaata
    38521 ctagaaatga caattctaaa atactagaaa tatataaaat gaagcttttt ggcgaacttg
    38581 taaaagcagt gtggaatgat gatatagagg atattgaaaa cttacctaat aaaattttaa
    38641 atgaagattt aaaggataat ttaaataatt attttagtgg aaaatttgat aaagatacta
    38701 tggtaaattt aattagggta actatgggat tggatgttac agaagataca gataaaaatt
    38761 taaaagagtc actgcatgaa gctgtaaatt tagatgaagt caaaagacca ataatatctg
    38821 taatatcaga tgcttgcaaa tattgtgatg aaaataataa ggaagatgag tcatgtaaga
    38881 taagaagtaa gcatattaat tgtaatgagg aagatgtatg tagtgcttgt ggtgattgtg
    38941 tatcaagatg taagttgggt gcaatttcag ataaaataga atttatacca atgataaaat
    39001 tgttaaaaga tataaagagc cctgtatatg caatagttgc accagctttt gtaggacagt
    39061 ttgggaaaga tgtaagccct ggtaaattaa gaactgcatt aaaaaagata ggatttgaag
    39121 atatgataga agttgctttg gcagcagata tgcttactgc aaaagaagca tatgattatt
    39181 atgagcatat gaaaaaggac gaagaaggat gctttataac tagttgttgc tgtcctattt
    39241 gggttagttt gatacaaaat agttttcctc agatattgga aaatgtatca ccatccgttt
    39301 ctcctatgat tgcttgtggg agagctgtaa aaattttaaa tccagatgct aaagttgtat
    39361 tcataggtcc ttgtatggca aaaaagaaag aagcaatgtt agaagatata aaagatgcag
    39421 ttgattttgt gcttacattt aaagagttgc aagaaatatt tgatgcatta gagttaaatc
    39481 cagcaataat ggatgagagt aatagagtag aggcgtccct ttcaggtaga gtttatgcta
    39541 gaacaggagg tgtaagtaaa gcagttaagt tatcagttag aagaattgac aaaaatataa
    39601 aatttgaaga aaaggcattt gaaggaacaa aagagtgcat ggacggtcta aagaaagttc
    39661 taaataaaga aatagaagct acatttatag aaggaatggg gtgtgtagga ggctgtgtag
    39721 gaggtccgaa gcgtatttta agtgtggaag aaggtactaa gaatgttgat gaatatagtg
    39781 aaagtacgaa tatggaaact ccatttgata atttaaatgt attacaattt ttaacttctt
    39841 tgggaatcaa aagagtagaa tctttaggag aaaaagaaga agaaaaggtt aaaaaaatat
    39901 ttagtagaga tataaaaagt aataagaaag agaataaata taaaaaataa ctttttaagt
    39961 cttaaactta tgcttttata aaagggagat atgggggatg aaaattaaag ataataaggc
    40021 tgtttttcat gtatttataa ggatatttct tatagctgtt ttaattttaa ctggaacatt
    40081 attggaggaa aaaattaata atgttccagt tgaaaatata agttcaaaag gtgaaaatac
    40141 actggaactg ctttctcaaa gtaatacttt aggagaaatc atatacaaag catctcaaga
    40201 agaaacaaaa ggtccagggg ctacttctga aaaagtagca tacataacaa tagatgatgg
    40261 tccatcaaaa tatacaaatg cactacttga tattttaaaa gaaaatgatg tcaaagcgac
    40321 atttttcatg cttaatcaga atatgaaaaa ccacaaggaa gaagtaagga gagtcttgga
    40381 agaagaaaac tcaataggtt ttcatagtgt aagtcatgat atacataagt tatataaaag
    40441 cccagaagta acagttgggg aatttaatac atgcaataat actctacaag aaataactgg
    40501 tcagagttca aaattaataa gattaccata cggaagtaaa ccatatatgc cagaaggttc
    40561 atataataaa cttatggcaa atgattattt aatttgggac tggaatttgg atacacagga
    40621 ttggaagtct tctacaagtc agatagtaag taatatatta tattatggaa gaaaaagaga
    40681 tgaattagta gtattaattc atgagaaaga gcaaagttta aatgctttaa ataatattat
    40741 aagaatctta aaagagagag gatatacaat attgccaatt acagaaaata taacacctaa
    40801 aaatttctgg caagaaaact tataataaat ttaaatatag taaaagataa agtaaattat
    40861 ttataaaata aaacatataa tataaaaaat acgaataaga ggtatactgt aaggataata
    40921 taaaattaat gagaaagcat taaaattaaa ataaaataat cttgcaatta ttgacattat
    40981 aagacagaat aactgtaaaa acatatttta aaatataaca actatataaa agagaataga
    41041 cataattaag aaaattcaga aaaaataaaa taaaaataat tgacaaagag aataatattt
    41101 gtatataata atatgtaata ttaaagactg agaagaagag gagtaagtaa atactactaa
    41161 aagagaggaa aattcactgg ctgtaagatt ttctaagtat aagatttact gaatgtagct
    41221 ttggagtttc taaactgaac tataagtaag tttagacggt tagaaccgtt aaattctttg
    41281 agagtcaaag ttgataaaat aacattaacc aataatttaa ttatatgtta atgtatttta
    41341 agctttggaa ttaaggtggt aacgcgagct tttcgtcctt tttaaagagg atgaagagct
    41401 cttttttatt tctaaagata tttaaaaaat ttatttataa aatacaggag gcaggacaat
    41461 ggaaaatacg aatttatcaa aaacttataa cccaaaagat tttgaggcta ggctatataa
    41521 aaaatggatg gatgaagggt attttaaatc taaaccaaat ccagataaaa aaccatttac
    41581 aataatgatg ccaccaccaa atataacagg gcaattgcat atgggacacg ctcttgacca
    41641 tacacttcaa gatatattaa tcagatggaa acgtatggat ggatatgaag ctttttggct
    41701 tccaggaact gaccatgctt ctatagcaac agaagttaaa gttgttgaaa gaataaaaaa
    41761 acaagaaggc aagacaaagt atgaaatagg tagagaagaa tttttaaaga gagcctggga
    41821 atggaaagat gaatttggag gaaaaatatc taatcagctt aaacaactag gtgattcttg
    41881 tgattgggat aaagaaagat ttactatgga tgaagggtgt aatgaagcag ttatagaatt
    41941 ttttgtaagc ctatatgaaa aaggtcacat atacagagga aatagaataa taaactggtg
    42001 cccagattgc aaaactactt tatcagatgc agaagttgaa catgaagaac acgacggaaa
    42061 cttttatcat ataaagtacc cactaaaaga tagtgaagac tttttagaaa tagctacaac
    42121 tagacctgag acaatgatag gagatactgg tatagcagta aatccagaag atgatagata
    42181 taaacattta ataggaaaaa cagcaatact tccattagta ggaagagaac ttccaatagt
    42241 tgcagatagt tatgttgatt tagagtttgg aactggtgca gttaagatga ctccagcaca
    42301 tgaccctaat gattttgaag taggattaag acataatctt gagcagttaa atactatgaa
    42361 tgaagatgga actatgaatg aagtttgtgg aaaatatgaa ggtatggata gatttgagtg
    42421 tagaaaagct atcgtagcag acttaaaaga acaaggttac ctaattaaga taaaagagca
    42481 taatcataat gtaggaactt gttatagatg ccatacagta gttgagccta gattatcaga
    42541 gcaatggttt gttaagatgg aagaacttgc aaaaccagct atagatattc ttaaaaaagg
    42601 agaattagaa tttgttccag ataaatttga taaaacttat ttacaatggc tagaaaatat
    42661 aagagattgg tgtatatcaa gacaattatg gtggggtcat caaataccag cttattattg
    42721 tcaagagtgt ggagaaatag tagttgctag aaaaatgcca gataagtgtc ctaaatgtgg
    42781 aagcactcat tttaaacaag atgaagatgc cttagatact tggttcagtt ctgcattatg
    42841 gcctttctct actctaggat ggccaaataa gactgaagct ttagattatt attatccaac
    42901 aagtgtactt gttacaggat acgatataat attcttctgg gtagttagaa tggcatttgc
    42961 aggtatgttc tgtatgaatg aaaagccatt tgaccatgtt ttggttcatg gattagtaag
    43021 agattctcaa ggtagaaaaa tgagtaaatc tttaggaaat ggtatcgacc cattggagat
    43081 aatcgagcaa tatggagctg atgccctaag atttacatta acaactggaa attccccagg
    43141 aaatgatatg agattctata tggaaagagt agaatttgct agaaactttg caaataagtt
    43201 gtggaatgca tcaagatttg tatttatgaa tattgatgaa gatataatta aaaacatgac
    43261 tagagaaagt gttaaagagg acttaactct agctgataaa tggataatat caagagcaaa
    43321 taatattgta aaagaagcaa ctaataacat ggataagttt gatttaggaa tagcacttca
    43381 aaagatatac gattttactt ggtcagaata ctgtgactgg tatattgaaa tggttaaacc
    43441 tagattatat ggggaagatg caaatgctaa atctgcagca ttatatacat taacttatgt
    43501 tcttgaaaag atattaaaat tattacatcc atatatgcca tttataacag aggaaatata
    43561 tactcatctt cctactgttg aaggatgtat aatagtatct gaatggccaa aatataatga
    43621 agaagataac atggctgaag aagaagatat gatgaactta ttgatggaag gtataagaag
    43681 tataagaaat gtgagagcag aaatgaatgt accaccttct aagaaagcta aattgataat
    43741 aatcccaagt gaagaaaaaa tagaagctat tgagcttggt aaagactatt ttataacttt
    43801 agcatctgct tcaaatgtag agatagcaaa agataaatct aatgttccag aagatgcagt
    43861 tggagtagtt attgatggtg ttgaaatatt tatacctctt aatgaattag ttgattttga
    43921 aaaagaaatt gagagattat ctaaagaaaa gaaaaagtta gaaggcgaaa taaagagagt
    43981 taatggaaag cttgcaaatc aaggtttctt agcaaaagct cctgaaagtt taattgaaga
    44041 agaaaaagca aaaaaagaaa aatttgaaga gatgattaaa tctgttgagg aaagattaac
    44101 aaatttagaa tctaaaataa aataaatata agaattagta aaaacataca aaaagtatat
    44161 attctatact ttttgtatgt ttttctttat atatatacaa atatgatgtt ataattcttt
    44221 acagatgtgt tatattttta ttttatgttg tgaatatatg aattttatgt cttaaaattt
    44281 ataagcaata aaaatttgat atattatgat gtttaatata gggtaataaa tacatattat
    44341 atttatttta ctatattttt ttacataata tatctagaaa agtataggaa tttatcgtaa
    44401 tttaagttta aatattaaag agaatttcta taagtagttt aatcgacaat ttaaatttat
    44461 aaagaaattt aaatttgaaa gctagtgagt acagtttgga aattgaaata aagtaaacta
    44521 aatgatattt tagatttgga ggtgatatta tgttcagggt agttatatgt gatgacgaaa
    44581 aaatacaaag aagcatacta aaagagttta cagaaaaact tttgtctgaa agatgtctaa
    44641 attatcaaat actggaattt tcctgtggag aagatttaat atccaaatac ccagaaaaaa
    44701 ttgatattat atttttagat atccaaatga aagatatcaa tggaattgag gtggctaaaa
    44761 aaataagaaa gtttgatgaa aaagtagaaa ttatatttac aacggctttt tcagagtatg
    44821 ccccccaagg ttatgaagtt cgtgcttata gatatttagt aaagcctata gagtatagta
    44881 actttgcaat gggtgtaaat ctttgtattg ataaccttca aagaaagaag gagcgttata
    44941 tagttttaaa tagtaaaaaa ggatttaata gaatactgat agattctatt ttatatgttg
    45001 aaactgtaaa gagagattta gttgtacata caataggtag agattataaa gctaatatga
    45061 gcatgaaaca agcagaaaaa ttactgtgtg aaaatggctt ttttagatgt catacaagtt
    45121 ttattgttaa tttaagaaga atagaagatc ttagggataa tacgattact attaatgaac
    45181 aatttatacc tataagtaag tatagactta aagatttaaa agtagcatta acaagcttgt
    45241 tatgtgatat aatgtattaa ttttaaaaag gtaattgaaa atatcgtaaa aaagatttaa
    45301 tgtcatcaca gggctattga ttgaaactgt cttaatatat aaaatgttat ttatattaag
    45361 acagttttat tatgcaaaat agcagtttat agacttttaa tatggtttaa tatgtatatg
    45421 tagagtttcc attataatag catgattatt aattagtagc ttgattatca atatactatt
    45481 attgattttt atatgtatta tatgtatttt tagaatatga ttaccttttt aaaataatga
    45541 ctatttataa aactctatag atatttgt
//
LOCUS       DAEPIN010000026        40697 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.026, whole genome shotgun sequence.
ACCESSION   DAEPIN010000026 DAEPIN010000000
VERSION     DAEPIN010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 40697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40697
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.026"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            <1..284
                     /locus_tag="KR250_001654"
     CDS             <1..284
                     /locus_tag="KR250_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HBH0067593.1"
                     /translation="MFLIYYVDDKHIKEIFEYCNNIQSEEYYVKMGMAWLLSICFIKC
                     EEETFLYIKNNNLDDFTYNKMLQKIIESNRVDLEKKNIIRSMKRKTRRC"
     gene            298..972
                     /locus_tag="KR250_001655"
     CDS             298..972
                     /locus_tag="KR250_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBH0067594.1"
                     /translation="MNNNEDRIDKFLELQSKGFKFAEIAQNLKISQSTLRTFLNKKGY
                     KSEKGKYIFKDELNYNKSLDNRNLEEEKAKEYEKSKKNTISNRKKVSTKKKISNEKAL
                     SKSVEQIEFNDTGLKNAKPKQNIKPKKDRKINITQEDLDKLCEVYDWYLEVKDYNIKP
                     KKVSNKKDVHIDDEHIDELKTTRIRIDKEIWNDFERLCSNSNFEKHQILTQALKEFMK
                     KYKNLL"
     gene            998..1834
                     /locus_tag="KR250_001656"
     CDS             998..1834
                     /locus_tag="KR250_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0067595.1"
                     /translation="MNKIVKKSISLMIILTIFIFMLTACEKDEQPDSVDIQTEDKNEI
                     KIDEGNAKVLNIGKSEIINIDEGDKVDTSTKVENNSTFNISNVELIYNEYDENKKIIS
                     SDSKALLDMTLMPGKVAYIECGHKTFVKGVEVYAYEYEAEGKIVYVNLKENTINIRNN
                     NIKLENSSQYEVLSTSELKKVNESNEGITYQVKVKNSSSKDLGNIILKTAEVNENGEY
                     LTVNRVPSYKILKPSEETDIDILCSTKAKSVEIVGYTYDDIKEKANVDIDLKSHKVKI
                     DK"
     gene            1850..2521
                     /locus_tag="KR250_001657"
     CDS             1850..2521
                     /locus_tag="KR250_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0067596.1"
                     /translation="MKVLIVEDNKILLESVVEELSKHFETEKCEDGEEALYLVNQNIY
                     DLVILDLMLPNINGFDILKKMRVNNIDTPVLILTAKETLDDKVEAFTIGANDYLTKPF
                     YMEELVARVYAILRTNGKIKERNGLEFKSLYLDTLEKRVYIEKEEIKLQNKQFNLLEY
                     FVLNKGSILLKEQIYDRIWGIDSDATIEIVEVYVSNLRKKLSKYGYDKYIKTKRKVGY
                     IFDDK"
     gene            2511..3743
                     /locus_tag="KR250_001658"
     CDS             2511..3743
                     /locus_tag="KR250_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBH0067597.1"
                     /translation="MINKNVFTSTKNHLIKMYIIVVGSFLIIFSIFIYSYFRGLTYSG
                     IDSEINDELEYIVSQFKRTSFLNPIRLKDPKDMVYVYEDGRISYYTQNEYFDELLPDR
                     RLDKKNSFFKYTENGYTFRELNVDVGRYQIQIIRNIDSEMNSLRQLTSVLIIGILISV
                     IITYFVAVYLTRKALIPIETAWKNQAKFIQDASHELRTPITIVSSKLESMLKSPESTV
                     NDEVETIATAMKETRRLKKMITDLLSLTKEDSIVKVNLEEIDLEKLLEEISEDYIDIA
                     EFQEKRFVFNSKLKNKVIITDKNKLRQLILIFIDNAFKYTKLGDEIALELKEDIEDEV
                     TLLISDTGIGIKKEEIPLIFDRFFRSENVRNKDLEGSGIGLSIARMISLNLSIDINVT
                     SDVDIGTTFELSIPKKLK"
     gene            3893..4210
                     /gene="trxA"
                     /locus_tag="KR250_001659"
     CDS             3893..4210
                     /gene="trxA"
                     /locus_tag="KR250_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HBH0067598.1"
                     /translation="MAKVINTSEFRSSVEGSKGVVVVDFFATWCGPCNMLGPVFAELG
                     EEMKDKARFVKVDIDESLEIAQQFNVSTVPTMIIFKDGKPVETLIGFMPKNKIEMQVK
                     SYL"
     gene            4227..5105
                     /locus_tag="KR250_001660"
     CDS             4227..5105
                     /locus_tag="KR250_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBH0067599.1"
                     /translation="MRYDIAIIGSGPAGLSAAINAKIRNKTIIMFGNDNLSNKLVKAP
                     SIDNYLGFYDISGDELKDKFKSHIDSMDISIENKRINNIYAMGEYFTIMSGNDMYEAT
                     TVILATGVEYTRPIKGEEEFLGRGVGYCATCDAPLYRNKKVAVIGYNEESKEEANFLS
                     ELTSKTYFIPMYKKDNLMRSSDNLDDSIEVIHDRPVQIDGDKLVNKVSFKENHIEVDG
                     VFVIKDSTAPSALVPGIEIDGIHIKVDNNMKTSIDGCFAAGDCVGKPYSYIKAAGQGQ
                     IAALNAVYYLDKLKRA"
     gene            5375..5743
                     /locus_tag="KR250_001661"
     CDS             5375..5743
                     /locus_tag="KR250_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBH0067600.1"
                     /translation="MENLVKIFKALSDETRLNILILVSKRNICQKGISKYLGISDSAV
                     SQHIKILKDVGIITGYKEGYYVLYHINKESFNICVDFINSMLSNSSESFIDVFDVNTI
                     HLGCSKECKSIKKCCKRREK"
     gene            5746..6117
                     /locus_tag="KR250_001662"
     CDS             5746..6117
                     /locus_tag="KR250_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HBH0067601.1"
                     /translation="MKVCIPVEENKGLDSKPYGHFGSAPIFVVCDLESGEVKSLDNGD
                     LDHEHGKCQPLKALSGTAVDAVVVGGIGQGAIIKLNGMGIKVYRAEGDTISANLDLLK
                     NGKLVEFPSDHTCSHDGCGHH"
     gene            complement(6262..6717)
                     /locus_tag="KR250_001663"
     CDS             complement(6262..6717)
                     /locus_tag="KR250_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0067602.1"
                     /translation="MNINTVQGDSIEVLLRQLGATRISKVSSTLYFIKFDLGDGWEIS
                     YTYNINAKDQYFLQRIEPYPIGRGLFNDEYEIVSFISKDLKKFLNAKNSSNFKTFVEV
                     TRKVNSIIDNVEELFLNYNVDGDDLKTLNKELNDILNDIDYIEKHTKKI"
     gene            complement(6756..8021)
                     /locus_tag="KR250_001664"
     CDS             complement(6756..8021)
                     /locus_tag="KR250_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HBH0067603.1"
                     /translation="MEFQQIIAICIFLIVMAAIITEKVNRSVAAVGGALLMIIFNILT
                     LDEGLSHIDFNTIGVLVGMMLFVAVVKNSGLFEYIAIWTAKKAKGDPWKIMICFAIIT
                     AILSAVLDNVTTVLLIGPMTIVITQILGLNPVPFLITQILASNIGGTATLIGDPPNIM
                     IGSAANLSFMDFVINLGPAVIVILAITIICFRFIYGKELVVNERAKNAILKLDEKKSV
                     KDKPLLIKSLILIAFILFGFMFHSTIHIDSSVVALTGASIMLLIGKQDVDEIMAGIEW
                     STILFFMGLFVVVGGLVEVGIINKLAQALIGLTEGHLVFTMLLILWLSAIVSSFLDNI
                     PFVATLIPLILTMQAEGIDVMPLWWATSLGACLGGNGTLIGASANVVLAGIGNKHGHP
                     ISFKEYFKIGFPLMIISIIISTVYLIIKF"
     gene            8491..9942
                     /locus_tag="KR250_001665"
     CDS             8491..9942
                     /locus_tag="KR250_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HBH0067604.1"
                     /translation="MDFFNGILTSINNAVVFVNNYLWSYILIIMLVVIGIYFTIKTNF
                     VQFRYFIEMFRLLGDGTANKNAKKEGKISSFQAFCISTSSRVGTGNIAGIAIAVVAGG
                     PGAVFWMWLIALIGSASSFVESTLAQIYKVKDGQAFRGGPAYYMEQGLNKKWMGILFS
                     ILITICYGFVFNAVQANTVSLAFNNAFGISKMAMGIGLGILTAIVIFGGVHRVAKVSE
                     IIVPVFAGLYILVALAIVVMNITEIPSVIALIVESAFDFKGMAIGTFMGVVMTGVKRG
                     LFSNEAGMGSAPNAAATAHVTHPVKQGLIQSLGVFTDTIIICSCTAFMVLLYSGYSSS
                     GATGIELAQEALTHHIGPVGNIFIAVCIFLFAFSSIVGNYYYGESNMEFMSGSKTKLN
                     VFRVFVVGMVLFGSLTQVDVVWNLADLFMALMAIINLIAIALLGKYAFIALKDYTSQK
                     KSGIKDPVFIADEIEGLEKVSEWHRELSEEHLG"
     gene            complement(10140..10781)
                     /locus_tag="KR250_001666"
     CDS             complement(10140..10781)
                     /locus_tag="KR250_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007491147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HBH0067605.1"
                     /translation="MTDNYIFDSYNNLVDGLSAYLGEGYEFILYKFNGVHYSINKIAN
                     GKYNTVKEGDIITDFDLALINKLENDADGDFISYFNRNKLGEPLKSTDIVIRGVDNKI
                     IGLLCIHFYLNTPLTRIIGSYIPEHNAMQSAPVLENIVEDTDEVILKSYQDAKVIVEN
                     DSNISSNDKNKSIVSILNESGIFKIKDSVVKVADLMGLSKNTIYMHLRNLNKN"
     gene            11255..12634
                     /locus_tag="KR250_001667"
     CDS             11255..12634
                     /locus_tag="KR250_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBH0067606.1"
                     /translation="MAQDKIVVEILENIGGKENIKEVAHCMTRLRIKVCDSSQVNQEK
                     LKKIKGVLGLVLKGEQYQIVIGPNVSQVYNTLNEVIGIIPEKDSKKTISEGKQNSIAK
                     ILDTIAGIFNPIVPALAGAGLIKAILALLVASKFLSADSETYIILNTISDGVFSFLPF
                     MLAYSAAKIFKMNTYVAVAIAGAMLHPNFINLIQQGLTKISFIGIPMSLIQYNGSVFP
                     IVLTIWFASYIEKGVDKITPKALKIILVPALTILFAAPIALIIIGPIAQMLGNYIAGG
                     ASILFERGGIFAGLIYGGIYSFMVVTGLHHGMVPVLVDGITRYGFNYISPVSGSSNMA
                     QAGSAFGVWLKAKDKDLKAVAATATISALTGVTEPAIYGVNLRLKKPFLCAAIGGAAG
                     GGFAAYFQCKAFAMGGPSFLTLPMFIGEGGHVGLVAIAFTIAFIVSAIATYIVGFEEE
                     VTADSHANL"
     gene            12678..13412
                     /locus_tag="KR250_001668"
     CDS             12678..13412
                     /locus_tag="KR250_001668"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010990049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HBH0067607.1"
                     /translation="MKIYLTKDYEEMSQVAAQMVLKYMYSNDNKRINLSITAGQTPKR
                     TYEILSEFVKNKPYFENVHYYNFDEIPIKGEKVGVTIRNLTNMYFREAQIPEERIHVL
                     DENNYQGFDRKIEADGGIDMVLMGLGWDGHFCGNLSGTVNSFDEETRAVSTYITDDMA
                     KLLTKSVGGNPEKLFDYYVTYGPKTIMHCKNIIMIVNGKHKAQILKQTLFGPLDLLCP
                     SSLLRLHPNFTLIVDEEAASEIKDLI"
     gene            13501..14700
                     /locus_tag="KR250_001669"
     CDS             13501..14700
                     /locus_tag="KR250_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006875852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBH0067608.1"
                     /translation="MYNHMKKVFQGLEGGLFSSVEKADVGDSYQKLGEQGVSLMGWAD
                     PFFPDYCIPRHILDAATLAISSNMSTHYTAPVGNDLLKIEIAEKLKEVNNLDVDFKRN
                     ILITPGSDSGLYYSMLPFINEGDEVLIPTPCYPNNIQNTKLMGGVPIFVELKESDGYQ
                     IDITEFEKRLTNKTKLVVLTHPNNPTTTIFNKESLEKLAKFIIDNDLILICDQAFEDF
                     TFENELITPASLEGMFERTVTIFSTSKGMGLSGFRVAYMVCSDKLMDKYYGAAVTVLG
                     ATNTVAQMSTIEAFKDRSFMEDFERVFDKRRQEAYKIINEIPNVSMQMPESGFLGWIN
                     VSKLGTSSEIQKYLVDEAKVCINDGINYGPGGEGHLRIILGSYKDDSKVIGALNRIKD
                     ALIKKQQ"
     gene            14724..15206
                     /locus_tag="KR250_001670"
     CDS             14724..15206
                     /locus_tag="KR250_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015529974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBH0067609.1"
                     /translation="MLSIFKSKKNDNKLKAFASGKVIPIEDVDDNVFSSKMLGDGIAI
                     YPTNEYVVAPCDAEVNIIMKDTKHAVGIKLFNGMEILFHVGIDTVSMNGEGFEIFVSP
                     GDKIKRGDKLISFNSKLIKEEGLQDVVIMVVTNSEQYKNIKYHIGLEAVIDDTIIAEI
                     "
     gene            complement(15524..16121)
                     /locus_tag="KR250_001671"
                     /pseudo
     CDS             complement(15524..16121)
                     /locus_tag="KR250_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816433.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(16365..16793)
                     /locus_tag="KR250_001672"
     CDS             complement(16365..16793)
                     /locus_tag="KR250_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0067610.1"
                     /translation="MKIHSIQNLTLDTRNLIKKYSLTLNEDLIWEFKHGKYHTIKYFT
                     HKFAVKESVLALLFNIHKLCYAKIKYFESSFSKYEPYKYSYQNEFVKCEMYDMNFMFH
                     KYSNIMIDLRNLQEIKTIEEFRLFCDYLESFEDNLQDLII"
     gene            complement(17194..17655)
                     /locus_tag="KR250_001673"
     CDS             complement(17194..17655)
                     /locus_tag="KR250_001673"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HBH0067611.1"
                     /translation="MIYEGKLIGKDLKIGIINSRFNEFITSKLLSGAEDCLLRHDVSP
                     ENIEIVWVPGAFEIPLVAQKMAKSGKYDAIICLGCVIRGATSHYDYVCSEVSKGIAKV
                     SLDSELPVIFGIVTTENIEQAIERAGTKAGNKGYDCAMNALEMANLFKSLD"
     gene            complement(17760..18953)
                     /locus_tag="KR250_001674"
     CDS             complement(17760..18953)
                     /locus_tag="KR250_001674"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HBH0067612.1"
                     /translation="MFNTIEEAIEDIKNGKMVIVVDDESRENEGDLLMAAEMATPESI
                     NFMATYGRGLICLPATEKKFKSLNIPLMVRENTDTFQTAFTVTIDGADTLTGISAYER
                     AETVKLFCDENSTSKDFKTPGHIFPLIAKTGGVLVRDGHTEASVDLARLAGFKEIGLI
                     CEIMKDDGTMARVDDLMIFKEKHNLKIITIKDLIEYRKINETTIEKVSSAFLPTKYGN
                     FEIIGYRDTYSNEEHIALTYGNINIENTLVRLHSECLTGDVFHSLKCDCGLQLESSMK
                     KIVENGSGVLIYMKQEGRGIGLLNKIKAYKLQEEGYDTVEANLMLGFEEDMRDFYMAA
                     QILKNLNIKSINLLSNNPDKINQLEKYGIKIENRIPINEEINDFNKLYLKTKKDKMGH
                     LLDII"
     gene            complement(18976..19641)
                     /locus_tag="KR250_001675"
     CDS             complement(18976..19641)
                     /locus_tag="KR250_001675"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HBH0067613.1"
                     /translation="MFTGIVEEVGILRKITANGKSGKVTILSNKILDGTNLGDSIAVN
                     GVCLTVSNLGKNEFTADVMMETIRSTNLGLLNANDKVNLERAMSLSSRFGGHIVTGHV
                     DGKGTICKFEKDENAVLVSIRPDKKLLSSMILKGSVAIDGVSLTISYLDDEIFKVSII
                     PHTKINTILLTKNVGDFVNLESDVIGKYVNNFMANNYKELNSNSSNHKSNIDKDFLFK
                     NGF"
     gene            complement(19675..20787)
                     /gene="ribD"
                     /locus_tag="KR250_001676"
     CDS             complement(19675..20787)
                     /gene="ribD"
                     /locus_tag="KR250_001676"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HBH0067614.1"
                     /translation="MNFVNQKEKDIYYMKKAIELAKNGEGFVNPNPLVGCVIVKDSNI
                     IGKGYHEKFGSNHAEVNAINSAKQSLKDSTLYVNLEPCSHYGKTPPCVDKIIQNKIKR
                     VVISTLDPNPLVCGNGVKKLKDNNIDVTVGILEDEARDLNEAFFYHIKNKRPLCIVKS
                     AVSLDGKIATKSLESKWISNESSRYLTHKYRNKYQSIMVGINTVLNDNPLLTCRLNQE
                     KVSHPTRIVIDTHLKLPLNSNLVKDKTSKTIVFTCCKESIKLSMLKENNVETIISPSK
                     NNLVDLEFVMYKLGELNIDSVLVEGGATLNDSLFRNKLVDKVKLFLSPKIIGGKDAPT
                     FVSGEGINHLSDSTQLTINNVTLIDGDILIESDVLN"
     regulatory      complement(20919..21040)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            21229..23711
                     /gene="recQ"
                     /locus_tag="KR250_001677"
                     /pseudo
     CDS             21229..23711
                     /gene="recQ"
                     /locus_tag="KR250_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729557.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
     regulatory      23800..23911
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            24053..25354
                     /gene="thiC"
                     /locus_tag="KR250_001678"
     CDS             24053..25354
                     /gene="thiC"
                     /locus_tag="KR250_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HBH0067615.1"
                     /translation="MNYTTQMDAARKGIITKEMEIVSQKEQVDVNELRELIANGQVVI
                     PANKNHKSLSAEGVGKNLRTKINVNLGISRDCKDIEKELEKVRVAIDMKAEAIMDLSN
                     YGKTREFREKVVEMSPAMIGSVPMYDAVEYLEKELKDITEEEFLNVIRQHAIDGVDFI
                     TIHAGLTRSVCQKIKNHERLTHIVSRGGSLLFAWMELNNKENPIYTNFDKILDICEEY
                     DVTLSLGDACRPGCIKDSTDGVQIQELVVLGELTKRAWERNVQVMIEGPGHMAIDEIE
                     ANVVLEKRLCHGAPFYVLGPLVTDIAPGYDHITSAIGGALACAKGVDFLCYVTPAEHL
                     RLPNLDDMKEGIIAAKIAAHAGDIAKNVKGAREWDNKMSKARADLDWCEMFRLAIDPE
                     KAKRYRDESTPTHEDSCTMCGKMCSMRTVKKILNNEELNLI"
     gene            25408..25602
                     /gene="thiS"
                     /locus_tag="KR250_001679"
     CDS             25408..25602
                     /gene="thiS"
                     /locus_tag="KR250_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HBH0067616.1"
                     /translation="MKVNGKEIEFEKDLTVIDLLNKYNLKSDRVVVEVNLEIIEESNY
                     NTYVLKDEDIVELISFIGGG"
     gene            25595..26398
                     /gene="thiF"
                     /locus_tag="KR250_001680"
     CDS             25595..26398
                     /gene="thiF"
                     /locus_tag="KR250_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine biosynthesis protein ThiF"
                     /protein_id="HBH0067617.1"
                     /translation="MVKIKIFVNELLTDVEENTTAYKVRDSYNNKCDVLVLNGYPIKT
                     DMTLCENDKLTLIQKGVKPSLDELESLLIARHTPNIHNKLKKGKVAILGLGGLGSNIA
                     ISLARIGVGELLLIDYDVVEPSNLNRQQYFIDDIGKLKTDAMIENIKKINPFIKLNKR
                     DIFLNKNNMDTIKDSDLIIEAFDDASCKAQVCNYVLINLKDKYLIASSGMAGYYDSNI
                     ITTKKIKDRFYICGDFVNEAKFGEGLMAPRVAICANHMANLATQILIDM"
     gene            26461..27231
                     /locus_tag="KR250_001681"
     CDS             26461..27231
                     /locus_tag="KR250_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HBH0067618.1"
                     /translation="MDKLVLGGHEFNSRLLVGTGKYGSNNILPEVIKESGSEIITMAL
                     RRVDLDNKQENILTYIPKEMTILPNTSGATNAEEAVRIARISRKMGCGDFIKIEVISD
                     TRYLLPDNEETIKATKILADEGFIVLPYMTPDLYAGRRLIEANAAAVMPLGAPIGSNR
                     GLQMKEMIRIMIDELDIPIIVDAGIGKPSQAMEAMEMGADAVLVNTAIASAGDPVQMA
                     RAFKLAVEGGREAYIAKTGNVSEFANASSPLTGFLGNL"
     gene            27248..28351
                     /gene="thiH"
                     /locus_tag="KR250_001682"
     CDS             27248..28351
                     /gene="thiH"
                     /locus_tag="KR250_001682"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HBH0067619.1"
                     /translation="MSFYDVIEKYRDFDFDGYLNNVTDNDVLRSLSKDKLEDFDILNL
                     LSKTAVKHLEDMAQKAHKLSVQYFGKTVCLYTPMYIANYCVNQCVYCSYNIKSGIKRK
                     KLTMDEIREEGEAISKEGFKHLLVLTGESSFHSSVEYIGEAIEILREKFPSIGIEVYP
                     MEVEEYKYIVDKGVEGLTVYQETYDEEIYKRVHIKGPKSNYKFRLDAPERGAKAGMRT
                     LSIGALLGLNDFRKETFFTILHGKYLKTKYPHIELSYSTPRMRPFKGCFEELVDISDT
                     DLVQAMVCMRLFDPHAAINISTRENLEMRSHIIPLGVTKLSAGVSTDVGGHSQDEHDT
                     AQFKINDESTVKDVEKMLNSIGYQHVFKDWERF"
     gene            28352..29041
                     /locus_tag="KR250_001683"
     CDS             28352..29041
                     /locus_tag="KR250_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HBH0067620.1"
                     /translation="MYLITNRKLCSEERYLEVIKESILSGVENIIIREKDLEYQELRK
                     LYMKIKTKINCIDFQEQISDESLKTNINQKECRNKFKVNFIINSNIEFFEKVDCQGIH
                     LPFKLFLNLIENKYNFNENKILGLSLHKVEEVDYLEKLIRNQNIKIDYITLSHIYETK
                     CKEGLNPKGIELLKEAKKITDIKIIALGGILPSNVKETLKYCDDFAIMSTIMRSKDIK
                     KTISNYNEKLN"
     gene            29229..30623
                     /gene="yfcC"
                     /locus_tag="KR250_001684"
     CDS             29229..30623
                     /gene="yfcC"
                     /locus_tag="KR250_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HBH0067621.1"
                     /translation="MTDSKKKRFIIPHTFTIIFLLIVLMAILTWIVPSGEFSREDVEG
                     RMVVVPGTYQSVESNPQGFADVFKAPIEGFIDSAEVVGFVLIVGGAFGIVNRTGAIEA
                     GIASVISKFKGKELLIIPISMILFGLGGTTFGMAEETLPFYMIFVPLMTSLGYDSLTA
                     IAIVFIGAAAGAAASTINPFSVGIAQALAQLAPGSGVTYRVVIFIAYILISIAFVMYY
                     AKKVKENPESSIVYEIDKNNKSILNQTSTEIKEFTFKEVSVLAIFGIGMAIMIWGVLS
                     QGWYIPEISMCFAAIGVLSGLVGRLSQIEISESFIDGAKDLASAALAIALARGIVIIA
                     QNGFIIDTILNSAAAFLGGLPKVIFVYLSFFLEAALAFLIPSSSGLASLTVPVLAPLG
                     DLVNISAQVIVTAYQFGVGLTNFITPTSGVLMGALAVAHIPFSKWVRFIMPILLILTA
                     VSLVFLTIGIYIGF"
     gene            31140..32507
                     /locus_tag="KR250_001685"
     CDS             31140..32507
                     /locus_tag="KR250_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HBH0067622.1"
                     /translation="MGLKEFDFIEESIDMLRTMSPTLETISDEIEEYFENILDEKNQE
                     YINVTSRIKSESSLREKIIRNRYLKKYGEASNLIHNVSDLIGLRIECRFIEDENKIYR
                     LLRRYFNKTDDKINYYNKENKNIKLKLSERQPNKQKNGFEIYKIDGVFAYLDREVKFE
                     LQIKSLVNVFWSEIEHKIIYKNNTYLLADKFIKDMMDSIKNNLTMIDNQLLSIYKNFH
                     SGKSFNMKVSKKEIEKLFAKLVYDAFSEKMNKSIGFVVDFKKPCETILNYSFNKQDIS
                     DEVLGNFMLDEFARLNEIVNKDIDFNEQIEFEREPTFDDKFCKDLGNHFRSRLNTEFP
                     WNLFFRILFEIEPYNNTKDFENFVEFIKENVLLEENREQLLCQFEEDSKLIIEDIYDC
                     IFNSISEIDSVEILYNYNLEKINFTSSEIINCICREYECYGEYLEEKEKLMNTFKEKI
                     IQIFE"
     gene            32532..33140
                     /locus_tag="KR250_001686"
     CDS             32532..33140
                     /locus_tag="KR250_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HBH0067623.1"
                     /translation="MRHFSNIYELSKIILEEAICIEKTGVILAGGRNSRMGRDKAFLE
                     LHDKLFIEIAIEAFKNFDELIIISNNEELYSKYDIKVYNDIVKDVGPIGGIYTALEYA
                     KYDIVTIACDMPYLNNQIVERIANKMNDKSVISVTNGKLQPLCSGYKKSIINKVSLCI
                     RENDLKLRSFIDKIDKSYIYFDEEDLFLNVNTVDEYEKLTKV"
     gene            33188..33559
                     /locus_tag="KR250_001687"
     CDS             33188..33559
                     /locus_tag="KR250_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HBH0067624.1"
                     /translation="MNLLKCDSWAVILNNSDKESKAYKILDELKRNMYKVVAIDEEKK
                     PIEGIDVYECLKDVPHNIDVVAIIDKQSKMDVILEEVELLDIQNMWFEKGSFSEMMIK
                     KTKDLKLNIEYNLSLYDELTR"
     gene            33738..34076
                     /locus_tag="KR250_001688"
     CDS             33738..34076
                     /locus_tag="KR250_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HBH0067625.1"
                     /translation="MLIVTTEKVEGKKISKVLGLVRGSTIRAKHVGKDIGASFKNLVG
                     GELTGYNEMLTEARQIAIGRMVEDAEAKGANAVIAFRLSSASVMQGAAEMLAYGTAVV
                     LEDDNSILEK"
     gene            34542..35045
                     /locus_tag="KR250_001689"
     CDS             34542..35045
                     /locus_tag="KR250_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HBH0067626.1"
                     /translation="MFNVAIITLSDKGYEGKREDITGKKLTEFVENTGAYKVTEYVLI
                     KDDKEMLKENIIRLCNSNKIDLILTNGGTGFSKRDITPEATKEVLEKEIPGLSEYMRM
                     KSTEITKKAILSRGVSGIRNNSIIINLPGSPKGAVENLSFIIDVLDHGIEVLRGEATE
                     CATKKSE"
     gene            35092..36051
                     /gene="moaA"
                     /locus_tag="KR250_001690"
     CDS             35092..36051
                     /gene="moaA"
                     /locus_tag="KR250_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HBH0067627.1"
                     /translation="MIDKYGRKIDYLRISLTDKCNLRCVYCMPPDVKFDKNYINENLS
                     FEDYKFIIKAMAEQGITKIRFTGGEPLLYPKLNELIKFTKEECGINNIGMTTNAIGLS
                     DRIFELEKCGLNKVNISLDSLKEYKYKSVTRGGNLKDVLKSIESCLNLGIKVKLNCVA
                     ISGFNDDELKDFMFLTVKSPIDVRFIELMPLGEANRVYKKGYFNVKEMIEKIYGLYKV
                     KNDEKSTAEYYMFKGAKGRIGIITPLSCSFCNTCNRIRLTSSGAIKLCLHSKEEIDIK
                     PFLHKPLLFRESMKDIIKQKPEKHHLIENKCSDTNRRMYEIGG"
     gene            36055..36525
                     /gene="moaC"
                     /locus_tag="KR250_001691"
     CDS             36055..36525
                     /gene="moaC"
                     /locus_tag="KR250_001691"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HBH0067628.1"
                     /translation="MLTHINEKGYAKMVDISEKDDTKRTAVATATITLSKEALEKVKN
                     GQIKKGDVLSVAQVAGIMGAKNTSSNIPMCHQIKLVGCDLEFDVDDSMSAIKIYATCK
                     SLGKTGVEMEALNACSLAALTIYDMCKAVDKKMVISNIHLVEKTGGKSGDFKFE"
     gene            36561..36992
                     /locus_tag="KR250_001692"
     CDS             36561..36992
                     /locus_tag="KR250_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HBH0067629.1"
                     /translation="MAKVVSINISEKKGVIKVPVQCAELKINHGIVGDAHAGNWHRQI
                     SLLGKESIDKMKMKGFDQLKYGDFAENVTTEGIEVFSLPVGTKLRIGQCEVEVTQIGK
                     KCHNGCEIKKLTGDCVMPREGIFVKVLKEGIINVNDSIDVI"
     misc_binding    37423..37634
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            37797..39086
                     /locus_tag="KR250_001693"
     CDS             37797..39086
                     /locus_tag="KR250_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HBH0067630.1"
                     /translation="MEEEKINYALEQIPENQKRGWVAMFSVLVAIGVDLSSVILGAEL
                     AQSMPMKQAILSVIVGSFFSAILYTTCSLVGSSTSLSTSMITKYVFGEAGAKIFSLVI
                     GVSLLGWFGVQVGFFAQNAQIIIKDIFNLDVSMQILSLIGGLLMMSTAIYGYKAMEKL
                     SVYSVPFLLVLMMLTIFLAFRANGISVDDNMKSTMTFAGGVSLSMSIIIVGAIVSPDI
                     SRWAKSRRDCALSSFLGIQFGNAFMIIVSIVLVKCMGTSDIMRIFITLGIAIPGIIVL
                     TLAQWTTNTSNVYSASLSIALVLKKAPEKVLTIVLGIIATLLAVFGIYEGFIGFLNLL
                     GIVIAPVGGVYTAEYYIVKQELKGFDKGVLYKPIVKRSIVSWIIGILITYLSTYGFIT
                     LTTIAPLDGFIAGFVVQSIIGKVLCSTKNKQEIDKAV"
     gene            39140..40219
                     /locus_tag="KR250_001694"
     CDS             39140..40219
                     /locus_tag="KR250_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF917 domain-containing protein"
                     /protein_id="HBH0067631.1"
                     /translation="MRFLTEESVDKIAVGAAVLGTGGGGDPYVGKLVAKQAIKKYGPV
                     KVISLDELDDDALVVPVSGMGSPVITIEKLLSEVELTTPLEIMEKLLNRKVDVIIPIE
                     IGGINSLMPIAVAAKKGLPILDADSMGRAFPEAQMVTFYLEGYEASPAVMSDEKGNSA
                     ILYPVDGIWSERLARTLTVEMGGSSSISDYNLSGAQVKKAAIGNTLTIAETIGGFLLE
                     NKSDSKGAVENILKELRGYKLFEGKVIDIKRELKGGFTRGHAFFAGINEYDGEYSILF
                     QNENLIAKKGDTPLCITPDLIAVLDLETGFPITTERIKYGSRVMVVAFPCNEKWRTEK
                     GIETVGPGYFGYDVEYKTVEELQGK"
     gene            40240..40605
                     /locus_tag="KR250_001695"
                     /pseudo
     CDS             40240..40605
                     /locus_tag="KR250_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423610.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
BASE COUNT        15763 a         4437 c         6867 g        13630 t
ORIGIN      
        1 ttatgttttt aatatattat gttgatgata aacatattaa agaaatattt gaatactgta
       61 ataatataca aagtgaagaa tattatgtta aaatgggtat ggcatggttg ctatcaattt
      121 gttttattaa atgtgaagag gaaacttttc tctatataaa aaataataat ttagatgatt
      181 tcacttataa taaaatgcta caaaaaataa ttgaatcaaa tagagtagat ttagaaaaga
      241 agaatataat aagaagtatg aaaagaaaga caagaagatg ttgacggagg aatattaatg
      301 aataacaatg aagacagaat tgacaaattt ttagaacttc aaagcaaagg atttaaattt
      361 gcagagatag ctcaaaatct aaagattagt caaagtactt taagaacttt tttaaataaa
      421 aaaggatata agtcagaaaa aggtaaatac atatttaaag acgaattaaa ttataataaa
      481 agtttggata atagaaactt ggaagaagag aaagctaagg agtatgaaaa aagtaaaaag
      541 aatactataa gtaatagaaa aaaagtaagc accaaaaaaa agatttctaa tgaaaaggct
      601 ttaagtaaaa gtgttgaaca aattgagttt aatgatacag gtttaaaaaa tgcaaagcca
      661 aaacaaaata ttaagcctaa gaaagataga aaaataaata taactcaaga agatttagat
      721 aagctttgtg aagtatatga ttggtattta gaagtaaaag attacaatat aaagcctaaa
      781 aaagtatcta ataaaaaaga tgtacatata gatgatgaac acatagatga attaaaaaca
      841 acaagaatac gtatagataa agaaatatgg aatgattttg aaagattatg tagtaattcg
      901 aattttgaaa aacatcaaat acttacacag gcattgaaag aatttatgaa aaagtataaa
      961 aatctactat aaaatactac tatatgaggt gcttaatatg aataaaatag taaaaaaatc
     1021 aatttctcta atgattatat taacaatatt tatttttatg ttgacagcat gtgaaaaaga
     1081 tgaacagcct gattcagttg atattcaaac agaagataaa aatgagataa aaatagatga
     1141 gggaaatgca aaagtattga atataggaaa atcagaaatt ataaatatag atgaaggtga
     1201 taaagtagat acatcaacaa aggtagaaaa taattcaaca tttaatatat ccaatgttga
     1261 attgatatat aatgaatatg atgagaataa aaaaattata tcttcggata gtaaggcttt
     1321 gttggacatg actttaatgc ctgggaaagt tgcttacata gaatgtgggc ataaaacttt
     1381 tgtaaagggt gtagaagttt atgcatatga atatgaggca gagggtaaga ttgtgtatgt
     1441 taatttaaaa gaaaatacta ttaatataag aaataataat attaagctag aaaatagtag
     1501 tcagtatgag gtgctatcta catcagaact taaaaaagta aatgaatcaa atgagggaat
     1561 tacgtatcaa gtaaaagtaa aaaattcatc atcaaaagat ttaggaaata ttattcttaa
     1621 gacagcagaa gttaatgaaa atggagaata tttaacagta aatcgtgtgc catcatataa
     1681 gattttaaag ccttcagaag agacagatat agatatattg tgttcaacaa aggcaaaaag
     1741 tgttgaaata gtgggatata cgtatgatga tataaaagaa aaagccaatg tggatattga
     1801 cttaaaatct cataaagtta agatagacaa ataaaggtag gggatactta tgaaggtttt
     1861 aatagttgaa gataataaga tacttttaga aagtgtagta gaagaactta gcaagcattt
     1921 tgaaactgaa aagtgtgaag atggagaaga agctttatac ttagtaaatc aaaatatata
     1981 tgatttagtc attttagact taatgcttcc taatataaat ggatttgata tcctaaagaa
     2041 aatgagagtt aataatatag atacacctgt acttatattg acagcaaaag aaacattaga
     2101 tgacaaagta gaagcattta ctataggagc taatgattat ttgacaaaac ctttttatat
     2161 ggaagagtta gtagctaggg tatacgctat attaaggact aatggaaaga taaaagaaag
     2221 aaacggatta gaatttaaat ctttatattt agatacacta gaaaaaagag tgtatattga
     2281 aaaagaagaa ataaaacttc aaaacaaaca gtttaacttg cttgagtatt ttgtattaaa
     2341 taaaggttca attttattaa aagagcagat ttatgataga atatggggaa ttgattcaga
     2401 tgcgactatt gaaatagtgg aagtatatgt aagtaatcta agaaaaaaac taagtaaata
     2461 tggatatgat aaatatatta aaactaaacg taaggtagga tatatattcg atgataaata
     2521 agaatgtatt taccagtact aaaaatcatt taataaaaat gtatattatt gtagtaggta
     2581 gttttttaat aatcttttct atatttatat actcatattt taggggactt acatatagtg
     2641 gaattgatag tgagataaat gatgaacttg aatatatagt ctctcaattt aaaagaactt
     2701 catttttgaa cccaataaga ctgaaagatc ctaaagatat ggtgtatgtg tatgaagatg
     2761 gaaggataag ttactatact caaaatgaat attttgatga gttacttcca gatagaagac
     2821 tagataaaaa aaattcgttt tttaaataca cagaaaatgg atatactttt agagaattaa
     2881 atgtagatgt aggtagatac caaatacaga taattagaaa tatagattct gagatgaatt
     2941 ctttaagaca gcttacatct gttcttataa ttggaatatt aatttctgta ataattactt
     3001 actttgtggc tgtatatctc actagaaaag ctttaattcc aatagaaaca gcatggaaaa
     3061 atcaagctaa gtttatacaa gatgcttctc atgaattaag aacaccaata acaatagttt
     3121 catcaaaatt agaaagtatg ttaaaatccc cagaaagtac agtaaatgat gaagttgaaa
     3181 caatagctac tgcaatgaag gaaactagaa gacttaaaaa aatgataact gatttattgt
     3241 ctcttactaa agaggactct attgttaaag ttaatttaga agaaatagat ttagaaaagt
     3301 tattggaaga aatatcagaa gactatattg acattgctga gtttcaggaa aaaagattcg
     3361 tgtttaattc taaacttaag aataaagtta taataacaga taaaaataaa ttaagacagc
     3421 ttatattaat tttcattgat aatgccttta agtatacaaa attgggtgat gagatagcat
     3481 tagaattgaa agaagatata gaggatgagg taactttact tataagtgac actggtatag
     3541 gcataaaaaa agaagagata cctcttatat ttgatagatt ttttagaagt gaaaatgtaa
     3601 gaaataaaga tttagaaggt tctggaatag gactttcaat agctcgcatg attagtttaa
     3661 acttatctat tgatataaat gtaacttctg atgtagatat tggaactaca tttgaattgt
     3721 caatacccaa aaagttaaaa taataaaaat ataaaaaatt gtttttagaa atgaagaatg
     3781 ggtatactaa ttagtataaa atataaaaag accttataaa agtaaattta tatttataaa
     3841 tttcatagta taattatata gggataataa agtttaatag gaggatttaa aaatggcaaa
     3901 agttataaat acatctgaat ttagaagtag tgttgaaggt agtaagggag tagttgtagt
     3961 tgatttcttt gcaacttggt gtggaccttg taacatgttg ggacctgtat ttgcagaatt
     4021 aggtgaagag atgaaggata aagctagatt tgtaaaagtt gatatagatg agagcttaga
     4081 aatagctcaa caattcaatg tatctacagt accaactatg attatattta aagatggaaa
     4141 accagtagaa actttaattg gattcatgcc aaagaacaaa attgaaatgc aagtaaaatc
     4201 atatttataa aaaatgggtg agtattatga gatatgatat agctataatc ggcagtggtc
     4261 cagctggatt gtctgctgct ataaatgcaa aaataagaaa taaaacaatt atcatgtttg
     4321 gtaatgataa tttaagtaat aagcttgtta aagctccttc aatagataat tatttaggtt
     4381 tttatgatat aagtggtgat gaattaaaag acaagtttaa atcacatata gatagtatgg
     4441 atatttcaat tgaaaataaa agaataaata atatttatgc tatgggagaa tactttacaa
     4501 taatgtctgg aaatgatatg tatgaagcta ctactgtaat ccttgcaact ggggtagagt
     4561 atactagacc aatcaagggt gaagaagaat ttctaggaag aggtgtgggc tactgtgcta
     4621 catgtgatgc accactttat agaaataaaa aggtagcagt tataggatat aatgaagaat
     4681 ctaaagaaga agcaaatttt ttaagtgaac tcacttcaaa gacttatttt atacctatgt
     4741 acaaaaaaga taatcttatg agaagttcag ataacctaga tgattctatt gaagttatac
     4801 atgacagacc agtacagata gatggagata aacttgtaaa caaagtatct tttaaagaaa
     4861 accacattga agttgatgga gtattcgtta taaaagatag tacagctcca agtgcattgg
     4921 ttcctggaat agagattgat ggcattcata ttaaagttga caataatatg aagactagta
     4981 tagatggatg ctttgctgct ggagattgtg ttggaaaacc atattcttat ataaaagcag
     5041 ctggtcaagg gcaaatagca gctttaaatg ctgtttacta tttagataaa ttaaagaggg
     5101 cttaatattt atattgatta actttttaaa gatatttata aaaaactgtc ttgaaataat
     5161 tttattattt caagacagtt ttttattttt aagttattaa taaaagtatt ttaaaatcta
     5221 tatgtaatta cttttattaa taaaactcaa agttatatta gtaaaaattg gaattattta
     5281 taatatactc caaaatttaa aaataatggt tgaaaaatct aattaagagt actataattt
     5341 aattaagtaa ttacttaatc aaaacgaggt acttatggaa aacttggtta aaatatttaa
     5401 agcattgagt gatgaaacta gacttaacat acttatatta gtatccaaga gaaatatatg
     5461 tcaaaagggg atttctaaat atctagggat ttcagattct gctgtttcac aacatataaa
     5521 aatattaaaa gatgttggga taataacagg atataaagaa ggatactatg ttttatatca
     5581 tattaataaa gaatcattta atatatgtgt agattttata aattctatgt taagtaattc
     5641 tagtgaaagc tttatagatg tatttgatgt aaatacaata catctaggat gcagtaaaga
     5701 atgtaaatct ataaaaaaat gttgtaagag aagggagaaa taataatgaa agtatgtata
     5761 ccagtagagg agaataaagg attggatagt aaaccatatg gacattttgg ttctgctcca
     5821 atttttgttg tgtgtgattt agaaagtgga gaagttaaat ctttagataa tggagattta
     5881 gatcatgaac atggaaaatg tcaaccatta aaagcattgt cagggacagc tgtagatgca
     5941 gtagttgttg gaggaatagg acaaggagct ataattaaat taaatggaat gggaataaaa
     6001 gtttacagag cagaaggtga tactataagt gctaatttag acttacttaa aaatggaaaa
     6061 ttagtagaat ttccatcaga ccatacttgc tcacatgacg gatgtggaca tcattaaaaa
     6121 aaaacagctg atgaaatata tacttcatca gctatttttt cttttaataa atacgatttt
     6181 attcttttaa aaccaaaatt tagattttta tattttttgt agtatcctta ataatatttc
     6241 aagtttacaa gtttactact tttagatttt ttttgtatgt ttttctatat agtcaatatc
     6301 atttaatatg tcattgagtt ctttattaag tgtttttaaa tcatcaccat ctacattgta
     6361 gtttaaaaat agttcctcta cattatcaat gatagaattg acttttctag taacttcaac
     6421 aaatgtctta aagttactac tatttttagc atttagaaat ttttttaaat cctttgatat
     6481 aaatgatact atttcatatt catcattaaa tagacctcta ccaataggat atggttcaat
     6541 tctctgtaaa aaatattggt ctttagcatt aatattataa gtataagata tttcccaacc
     6601 gtctcctaaa tcaaacttga taaaatataa tgtactactt acttttgata ttcttgtggc
     6661 accaagctgt cttaaaagaa cttcaatcga atcaccttga acagtattaa tattcatata
     6721 tacacatccc ctttagtatt attatactat attttttaaa attttataat taaataaaca
     6781 gtagatataa taatagatat aatcataagt ggaaaaccta ttttgaaata ctccttaaaa
     6841 gagataggat gaccgtgttt attacctata cctgctagta caacattagc agatgctcct
     6901 attagtgttc catttcctcc aagacaagct ccaagagatg ttgcccacca tagtggcatt
     6961 acatcaatac cttcagcttg catagtcaaa ataagaggaa ttagtgttgc aacaaatgga
     7021 atattatcta agaaagaaga gactatagct gacaaccata atatcaacaa cattgtaaat
     7081 acaagatgtc cttctgtaag tccaatcaat gcttgagcca atttgtttat tatcccaact
     7141 tctactaatc caccaactac tacaaataat cccataaaga ataaaattgt agaccattca
     7201 atacctgcca taatttcatc tacatcttgt tttcctatta aaagcattat agatgctcct
     7261 gtcagtgcta caacagaaga atctatgtgg attgtactgt gaaacataaa accaaaaagt
     7321 atgaatgcta ttaatataag gctttttata agtagaggtt tatctttaac agattttttt
     7381 tcatctagtt ttaatattgc atttttagct ctttcattca caacaagttc ttttccataa
     7441 ataaatctaa agcatattat agttatagca agtattacta taacagcagg acctaaattt
     7501 attacaaaat ccataaaact aagattggca gcactaccaa tcataatatt tggagggtct
     7561 cctataagag tagctgttcc tcctatattt gaagctaaaa tctgtgttat taaaaatgga
     7621 actggattaa gtcctaatat ttgtgtaata actatagtca ttggtccaat aagtaagacg
     7681 gttgtaacat tatctaatac agctgataaa atagctgtta taatagcaaa gcaaatcatt
     7741 atcttccatg ggtcaccctt agctttcttt gcagtccaga tagctatata ttcaaataac
     7801 cccgagtttt tgacaacagc tacaaatagc atcataccaa ctaatacacc aatagtattg
     7861 aaatctatat ggcttaatcc ttcatcaagt gtaagtatat tgaatattat cattaatagt
     7921 gcaccaccaa cagctgctac ggatctgttt accttttctg taataatagc tgccataact
     7981 attaaaaata tacatatagc tattatttgt tgaaattcca taatataagt acctccattt
     8041 gtattatttt gtgcctaatg ctagtactat atatagtatt acttttttat tgttttttca
     8101 atggatgtat gaaagtatat tatttaacaa agtttttgaa aaattgacag aatttaaaga
     8161 aaatttttgt tatttaaata aattttaaaa ttattccatt gataataata ttaaacgaca
     8221 aacgaaaaaa tatacccttt aaactagaat ttattgtatc taataacaat tataaaatat
     8281 taaatatatc tatattataa aaacaataaa aaaaggtttg atttgaatat aaaactgcaa
     8341 tatattaaaa aaattattca ttttaaacct tgtaatttga ttttataaat attataatta
     8401 aattaatgaa tttttaacaa ggaattattc atttttttga ttttaagatt tattgagaaa
     8461 acaaaactaa ataggggggt aattaatata atggattttt tcaatggtat tttaacgagt
     8521 attaacaatg cagtagtatt tgtaaacaat tacttatggt cgtatatctt aattataatg
     8581 ttagttgtaa ttggtattta ctttactatt aaaacaaatt ttgtacaatt cagatatttt
     8641 atagaaatgt ttagacttct gggtgatggt acagcgaata aaaatgcaaa aaaggaagga
     8701 aaaatctctt catttcaagc attttgtata agtacatctt caagagttgg aactggaaat
     8761 atagctggaa tagcaattgc agtggtagca ggaggacctg gtgctgtatt ttggatgtgg
     8821 cttatagcat taattggttc tgcatcaagc tttgttgaga gtacattagc tcagatttac
     8881 aaagtgaaag atggtcaagc ttttagaggt ggaccagcgt attatatgga acaaggtcta
     8941 aataaaaaat ggatgggtat tttattctca atacttataa ctatatgtta tggttttgta
     9001 tttaatgcag ttcaagcaaa tactgtttca cttgcattta ataatgcatt tggaataagt
     9061 aaaatggcta tgggaatagg tcttggaatt ttaactgcaa tagttatttt tggtggagtg
     9121 catagagttg caaaagtttc tgaaataata gttcctgtat ttgcaggatt atatatatta
     9181 gttgctttgg ctatagttgt aatgaatata acagagatac caagtgttat agcattgata
     9241 gttgaaagtg catttgattt taaaggaatg gcgattggta catttatggg agttgtaatg
     9301 actggagtga agagaggtct attttctaat gaagcaggta tgggtagtgc tcctaatgct
     9361 gctgcaacag cacatgtaac tcatcctgta aaacaaggtt taattcaatc tttaggtgtt
     9421 tttacagata ctataataat atgtagttgt acagcattca tggtattatt atattcaggt
     9481 tatagtagta gtggagcaac tggaattgaa ttagctcaag aagctttaac tcatcatata
     9541 ggaccagttg gaaatatatt tatagcagtt tgtatattct tgtttgcatt tagttctata
     9601 gttggtaatt actattatgg tgaatcaaat atggagttta tgagtggaag taagactaaa
     9661 cttaatgtat ttagagtttt tgttgtaggt atggttttat ttggttcact tactcaagtg
     9721 gatgtagttt ggaatcttgc agacttattt atggcattga tggcgataat aaacttaata
     9781 gcaatagcac ttttaggaaa atatgcattt attgctttaa aagattatac aagtcaaaaa
     9841 aaatcaggaa taaaagaccc tgtgtttatt gcagatgaaa tagaaggcct agaaaaggta
     9901 tcagagtggc atagggaatt atctgaggaa catttaggat aaaaaatagt ataaaattat
     9961 aaaaccattt tgaaagacta gagtatgtat atactttata tgaatataat attgaattaa
    10021 tttatattac tggaattttt aattccagta atattttttt aattacaagg atattatgat
    10081 gacttaaaga ctgtggaaaa ctctttttta gttttcatta ttaatattaa ttgacaaatt
    10141 tagtttttat ttaaatttct taagtgcata taaatcgtat tttttgataa tcccattaag
    10201 tctgcaactt taacaacaga atccttaatt ttaaaaatac cactttcatt taaaatagaa
    10261 acaatagatt tatttttatc attgctagat atattagaat cattttcaac aattaccttt
    10321 gcatcttggt atgattttaa aattacttca tcagtatctt ctacgatatt ttctaaaaca
    10381 ggagcacttt gcatagcatt atgttccgga atatatgaac caattattct tgttagtggt
    10441 gtattaagat aaaaatgtat gcagagtaga ccgattattt tattatcaac tccccttatc
    10501 actatatctg ttgattttaa aggttcacct agtttgtttc tattaaaata actgataaaa
    10561 tctccatcag catcattttc gagtttatta atgagtgcca aatcaaaatc agttataata
    10621 tctccttctt ttacagtatt atatttacca ttagctattt tatttattga atagtgtact
    10681 ccattaaatt tatataaaat aaactcgtat ccttctccta aatacgcaga taatccatcc
    10741 actagattgt tataagaatc gaaaatatag ttatcagtca tgttaaaagt catatgttac
    10801 ctcctaagaa atatcttaat atcatattaa catataaaat gaatttaata aaatatattt
    10861 cagtgaaata ataaatataa tgaaaaaaaa actattgact gaaaaatatt tcagtgatat
    10921 tattaaaata cagtaaggca aacgtattat tgacagaata tataaaatgt taaaaatagt
    10981 atctaggaat taaacaaatt aaaggactag tgatttgaat ttttttagta taatatgtga
    11041 cttccaatta atatttatta aacaccaata aaattgtaaa tatgcttaat gatatacttt
    11101 agcgatgaag tgaaaatatt ttatttttat gattaaccta taggaattaa tttagcatac
    11161 tgtttagcta gtaaatctaa ataaatttta gctttagctg atatcatgtt gaataaagtt
    11221 tctattggat tttgtataac aaaggagtgg ggagatggca caagataaaa ttgtggttga
    11281 aattttagaa aacattggtg gtaaggaaaa tattaaggaa gtagcacatt gtatgactag
    11341 actgagaata aaggtatgtg atagttctca ggtaaatcag gaaaaactaa agaaaataaa
    11401 aggtgtacta ggtcttgtat taaaaggtga gcaatatcaa attgttatag gtcctaatgt
    11461 atcacaagtg tataatactc ttaatgaagt tataggaatt atacctgaga aggatagtaa
    11521 aaaaacaata tctgaaggta aacaaaatag tattgcaaaa atattagata ctattgcagg
    11581 tatttttaat cctatagttc cagcattagc gggtgcaggt ctaataaaag ctatattagc
    11641 attactagta gcatcaaagt ttttatctgc agatagtgaa acctatataa tattaaatac
    11701 aatatctgat ggtgtatttt catttttacc atttatgtta gcttactcag cagctaaaat
    11761 atttaaaatg aatacttatg ttgctgtagc aatagcagga gcaatgctac atcctaattt
    11821 cattaattta attcaacaag gcttaactaa aatatctttt atcggtattc cgatgtcact
    11881 aatccagtat aacggaagtg tatttccgat agtactaact atttggtttg catcgtatat
    11941 agaaaaagga gtagataaaa taacaccaaa ggcacttaag ataatattag tacctgcatt
    12001 gactatttta tttgcagctc ctatagcatt aattattatt ggaccaatag ctcaaatgct
    12061 aggaaactat atagccggtg gagcaagtat cctatttgaa agaggaggaa tatttgcagg
    12121 gttaatttac ggaggaattt attcattcat ggtagtaaca ggattacatc atggtatggt
    12181 tcctgtatta gtagatggaa taacaagata tggatttaac tatataagtc ctgtgtcagg
    12241 tagttcaaat atggctcaag caggatctgc atttggagta tggttaaaag caaaagataa
    12301 agatttgaag gctgtagcag ctacagctac catatcagca ttaactggag ttaccgaacc
    12361 tgcaatatat ggtgttaatt taagattaaa gaaaccgttt ttatgcgctg ctataggtgg
    12421 tgcagcagga ggtggatttg ctgcatattt ccaatgtaaa gcatttgcta tgggaggtcc
    12481 atcgttttta actcttccta tgtttatagg agaaggtgga catgtaggat tggtagcaat
    12541 agcatttaca atagcattta tagtatctgc aatagctacc tatatagtag gatttgaaga
    12601 agaggtaaca gcagattcac acgctaactt ataataaaga gaaattattt aacatatcaa
    12661 aaaggagatt aattactatg aaaatttatt taactaaaga ttatgaagaa atgagccaag
    12721 tggcagcaca aatggtgtta aagtatatgt acagcaatga taataaaaga ataaaccttt
    12781 ctataacagc aggtcaaaca ccaaaaagaa catacgaaat attaagtgaa tttgttaaga
    12841 ataaacctta ttttgaaaat gtacattatt ataactttga tgaaatacca ataaaaggcg
    12901 aaaaagttgg agttactata agaaatttaa ctaatatgta ctttagagaa gctcaaatac
    12961 cagaagaaag aatacatgtg ttagatgaaa ataattatca aggctttgat agaaagatag
    13021 aagccgatgg tggaatagac atggtattaa tgggattagg ttgggatgga cacttctgtg
    13081 gaaatcttag tggtacagtc aactcttttg atgaagagac aagagctgtt tcaacttata
    13141 taacagatga tatggctaaa ctattaacaa aatctgtagg tggaaatcca gaaaaattat
    13201 ttgattacta tgtgacatat ggacctaaaa caataatgca ttgtaaaaat ataataatga
    13261 tagtaaacgg aaaacacaaa gctcaaatat taaagcaaac actatttgga ccattagatt
    13321 tattatgtcc atcatcttta ttgagattac atcctaactt cacattaata gtagatgaag
    13381 aagcagcatc tgaaataaaa gatttaatat aaataagtaa ggaattaaaa ttaaaaatga
    13441 atttaggtgc aaagtaatat tttattttgc acctttgctt taataaaaag gagaataaaa
    13501 atgtataatc atatgaaaaa agtattccaa ggattagagg gtgggttatt ttcaagtgta
    13561 gagaaagctg atgtaggcga ttcttatcaa aaattaggtg agcagggtgt aagtttaatg
    13621 ggatgggcag atccattttt cccagattac tgtataccta gacatatatt agatgcagct
    13681 acattagcga tatcaagtaa tatgtcaact cattatacag cgcctgtagg taatgactta
    13741 ttaaaaattg agatagcaga aaaattaaaa gaagtaaata atttagatgt tgattttaaa
    13801 agaaatatat taataactcc tgggtcagac tcagggcttt attactcaat gttaccgttt
    13861 ataaatgaag gggatgaggt tcttatacca acaccatgct atccaaataa tatacaaaat
    13921 actaaattaa tgggaggagt tcctatattt gtagagttaa aggaatcaga tggatatcaa
    13981 atagatataa cagaatttga gaagagatta actaataaga cgaagttagt agttcttact
    14041 catcctaata atccaacaac tacaatattt aataaagaat ctttagaaaa attagctaaa
    14101 tttattatag acaatgattt aatattaata tgtgatcaag catttgaaga ttttactttt
    14161 gaaaatgaat taataacacc agcttcatta gaaggtatgt ttgaaagaac agtaacaata
    14221 ttttcaacat caaaaggtat gggattaagt ggatttagag ttgcatatat ggtatgcagc
    14281 gataaattaa tggacaaata ctatggagca gcagtgacag tattaggtgc tactaataca
    14341 gtagctcaaa tgtcaacgat tgaagcattt aaagatagaa gctttatgga ggattttgaa
    14401 agagtttttg ataaaagaag gcaagaagca tataagataa tcaatgaaat acctaatgta
    14461 tctatgcaga tgcctgagtc cggattttta ggttggataa atgtatcaaa gcttggaaca
    14521 tcatcagaaa tacaaaagta tctagttgac gaggctaaag tttgtattaa tgatggaatt
    14581 aattatggtc caggaggaga agggcatttg agaataattt taggaagcta taaagacgat
    14641 agtaaggtaa ttggtgcatt aaatagaata aaagatgctt taattaaaaa gcaacaataa
    14701 ataaaaaata attggagtgg agtatgttat caatatttaa gagtaaaaaa aatgacaaca
    14761 agcttaaggc atttgcatca gggaaagtaa ttcctataga agatgttgat gataatgttt
    14821 tttcatctaa aatgttaggt gatggaatcg ctatttatcc aactaatgaa tatgttgtag
    14881 ccccttgtga tgcagaagta aatataataa tgaaagatac taaacatgca gttggaatta
    14941 aattatttaa tggtatggaa atcttatttc atgttggaat agatactgta tctatgaatg
    15001 gagaaggttt tgagatattt gtttcaccag gtgacaagat aaaaagaggt gataaattaa
    15061 tatcttttaa tagtaaatta ataaaagaag aagggttaca agatgtagtt ataatggtag
    15121 ttactaattc agaacagtat aaaaatataa aatatcatat tggactggag gcagtaatcg
    15181 atgatactat tattgcagaa atttaaaaac agttatttaa gtaaatactt agataattta
    15241 gatatagtta aaattaaatc cttaaaatca aattatttaa tttttgtgta attttaacta
    15301 taaagccgat taataatata aagtatacta aataatatgg ttattatgat tgttactgac
    15361 aaagcctaag tagatataac aatttgctct aattgagtat tgctatatct acttgttttt
    15421 atatttaaaa taaaagagtc tgcattgaaa agataattct gatgagagta aatagggtat
    15481 ttaataatag tttaattctg acttatcaag atgaacctat tcatatacct aaaacatctt
    15541 taacaccttc taaattcata caaatagctg tataggctgc cttattgatt atttttccgt
    15601 caatttttac tttgtagtga acagcatata taaatattat cgaatagatt gtatcaagag
    15661 ctctattttg ctattcatta gctaaagata gtactctgtc agttattttt gaaactaatg
    15721 catgagatat atcaattccg taaatattat ttaaatgtat gatatatatc gcgagtcgac
    15781 attccttttg agtacatgcc aattacttgg ttctctattc cagaaatttc attttcgtaa
    15841 ttttgaataa ccctaggctg gaaatcatcc ctagggatat ttaattttac ctctccaaaa
    15901 tctgatttaa cattcttagc tctatatcca tttcttgaat ttgatgttgc tttactttgg
    15961 ttatcatatc tctcgtacat taaatgatct tcaatctctg actctaacat tttgtgaatt
    16021 gtgcttgcga ataaatcttt taacatctct tggatgtctt tagtattttt tacatcatac
    16081 tcttgaacta agtgttttaa tatttaattt ttgttgtaca taaaaaatgt gtacctccta
    16141 ttttggtaat tagatttaat atcttgactt ttaggagaat acacatttta ttttacagac
    16201 cctcttttct agaaaagttt ttatgaaaat tgtgatataa attattaata aaaaataatt
    16261 ttatatttat tttattatgt aatttagttt tattaaaaat tttttattat tggatataga
    16321 ttatctatta taaattttat ctttaagttt attctcctaa aatactatat aattaaatcc
    16381 tgtaaattat cttcaaagct ttctaggtaa tcacaaaata atctaaattc ttctatggtt
    16441 ttaatttctt gtaaatttct caaatctatc attatattac tatatttatg aaacataaaa
    16501 ttcatatcat acatttcaca tttaacaaat tcattttgat aactatattt ataaggttcg
    16561 tattttgaaa aactcgactc aaaatatttt attttagcat aacataattt gtgtatgttg
    16621 aacaaaagtg caagaacaga ctccttaact gcaaacttat gtgtaaagta ttttatcgtg
    16681 tggtatttac catgtttaaa ctcccaaatt aaatcctcat ttaaagtaag agaatatttt
    16741 tttataaggt tacgcgtatc tagtgttaaa ttttgtattg aatgtatttt cataatttat
    16801 tctcctcata aatatttaat aagtattaaa ttatagtgtt ttattattaa ttgttcttaa
    16861 ttatattgta tggtaaatta aaaaagtaat aaaatagata attaatatac tagtaatata
    16921 tgctataaat aaaaacaata gctaaaatag taatatatat gtttttatga tattttatgt
    16981 ttagggttat atttgtaatt attttaagct tctaaaagta gatttgatgg ataacgtata
    17041 attttagaag gatatgcaat ttgtttgata agaacgtaca tatgattaaa ttaaaaatat
    17101 taagtaccaa aaagaagaaa gatagtttct ccctttggta cttaatattt tgtatccttt
    17161 acactaattt ttaatattta ttaatgatat tatttaatct agagacttaa ataaatttgc
    17221 catttctagc gcattcatag cacaatcata acctttattt ccagcttttg tacctgctct
    17281 ttcaatagct tgttcaatat tttctgtagt tacgattcca aatataacag gtaattcact
    17341 gtctaaagat actttggcta tccctttaga gacttcacta catacataat cataatgaga
    17401 agtagcccct cttataacac agcctaaaca gattatagca tcatacttac cacttttagc
    17461 cattttttga gcaacaagag gaatctcaaa agctccaggt acccaaacta tttctatatt
    17521 ttctggagaa acgtcatgtc ttaaaagaca gtcttcagct cctgataaaa gttttgatgt
    17581 tataaattca ttaaaacgag aatttattat acctattttt aaatcttttc caataagttt
    17641 tccttcatat atcattttta cttcctccaa ttttatttat gttatagatt tatatttata
    17701 aatatcttta atttaattat aaaaaactac ttagtagaaa aatatcatca tataatcatt
    17761 tatataatat ctaaaagatg tcccatttta tcttttttag tttttaaata tagcttatta
    17821 aagtcattaa tttcttcatt aatagggatt ctattttcga tttttattcc atatttctct
    17881 aattgattta ttttatctgg gttattactt aaaagattta tactttttat gtttaaattt
    17941 tttaatattt gagcagccat atagaaatct ctcatatctt cttcaaatcc aagcattaaa
    18001 tttgcttcaa cagtatcgta accttcttct tgaagtttat aagccttaat tttatttaaa
    18061 agaccaatac ctctaccttc ttgcttcata taaattaaaa ctccacttcc attttccaca
    18121 attttcttca tagaagattc tagctgtaat ccacaatcac attttagaga gtggaacaca
    18181 tctcctgtta aacattcaga atgaagtcta actagagtat tttctatatt tatatttcca
    18241 taagtaaggg caatgtgttc ttcatttgaa taagtatctc tatatcctat gatttcaaaa
    18301 tttccatatt ttgtcggaag gaaagcacta gatacttttt ctatagtagt ttcatttatt
    18361 ttgcggtatt ctataaggtc ttttatagtg atgattttta aattatgctt ttctttgaaa
    18421 atcattaaat catcaactct tgccattgta ccatcatctt tcattatttc acagataaga
    18481 ccaatctctt taaaacctgc aagtctagca aggtctactg aggcttctgt atgtccatcc
    18541 ctgacaagaa ctccaccagt ttttgctatt aaaggaaaga tgtgaccagg cgttttgaaa
    18601 tctttacttg tagaattttc atcacagaat aattttacag tttcagctct ttcatatgca
    18661 gatataccag ttagagtatc tgctccatct atagtaacag taaaggctgt ttgaaatgta
    18721 tctgtatttt ctctaaccat aagaggtata ttaagacttt taaatttttt ttcagtggca
    18781 ggtagacaaa ttagacctct accatatgta gccataaaat ttattgattc aggagtagcc
    18841 atttcagctg ccatcaataa atctccttca ttctctctac tttcatcatc aactacgatt
    18901 accattttcc cattttttat atcttcaata gcttcttcaa tagtattaaa catgtaattc
    18961 actcctttat tatttttaaa aaccattttt aaataaaaaa tctttatcta tatttgattt
    19021 atggttacta ctattagaat ttaattcctt gtagttatta gccataaaat tattaacata
    19081 ctttccaatt acatcgcttt ctaaattaac gaaatctcca acattctttg tcaagagaat
    19141 agtgttaatt ttagtatgag gtataataga aaccttaaat atttcatcat ctaaatatga
    19201 tatagttaaa cttacaccat caatagcaac agaacctttg agtatcattg aggataaaag
    19261 ttttttatca ggtcgtatac ttactaaaac tgcattttca tctttttcaa atttgcagat
    19321 agttccttta ccatcaacat gacctgttac tatatgacca ccaaatcttg aagaaagact
    19381 catagctctt tcaagattta ccttgtcatt tgcatttaat aaacctaaat ttgtggaacg
    19441 aattgtctcc atcataacat cggctgtaaa ttcatttttt ccgaggtttg aaacagttag
    19501 acaaactcca tttacggcta tactatctcc aagatttgtg ccatctaata ttttatttga
    19561 tagtatggtt acttttccac tcttaccatt agcagtaatt tttctaagta ttccaacttc
    19621 ttctactatg ccagtaaaca tcctaacctc ctatatgaat gtattaacaa aaatttaatt
    19681 taaaacatct gattctataa gaatatcgcc atctatcaaa gttacattat tgattgttaa
    19741 ctgtgttgaa tctgacaaat gatttattcc ttctccagaa acgaatgtag gtgcatcttt
    19801 accacctata atcttgggtg atagaaataa tttgacttta tcaactaact tatttctaaa
    19861 gagactgtca ttgagagtag caccaccttc aactagaaca gaatcaatgt ttaactctcc
    19921 taatttatac attacaaact ccaaatctac taagttgttt tttgaaggag aaattattgt
    19981 ttcaacatta ttttctttta acatagataa ttttatactt tctttgcaac atgtgaaaac
    20041 tatagtttta ctggttttat ctttaaccaa attactatta agtggcaact ttaggtgtgt
    20101 atctattact attcttgtag gatgagacac tttttcttga ttgagcctac aagttagtaa
    20161 aggattatcg ttcaaaactg tatttatgcc aaccattata ctttggtatt tatttctgta
    20221 tttatgagtt agataccttg atgattcatt agatatccat tttgattcta gtgattttgt
    20281 ggcaatttta ccatctagac ttactgctga ttttacaata caaagaggtc ttttgttttt
    20341 tatatgataa aaaaatgcct catttaaatc tcttgcttca tcttctaata ttcccacagt
    20401 aacatcaata ttgttatctt ttaatttttt tactccatta ccacaaacaa gtggatttgg
    20461 gtctaatgta gatattacaa ctctcttaat cttattttgt ataattttat ctacacaagg
    20521 aggcgttttc ccataatgag aacatggttc taagtttaca taaagagtag agtcttttag
    20581 actttgttta gcactgttta tagcatttac ttcagcatga ttagagccaa atttctcatg
    20641 atatcctttg cctattatat tactatcttt aacaattaca catccaacta atggatttgg
    20701 atttacaaag ccttctccat ttttagccaa ttcaatagct tttttcatat aataaatatc
    20761 tttttctttt tgattcacaa aattcacaat atgtacacct cctgtttaat tggataattt
    20821 ttaaacaaaa aaccctgaga attaactcag ggtttagaat aatctacaat aaatgtttaa
    20881 taaatgtaga acaaaattgc tctacaaaaa cactcattaa atatcttctt ccatccagac
    20941 tttactgtcg gctttggaat ttaaccaaat caaccaagtt ttacattggc tcgcgggcta
    21001 taccgccgat tgggaatttc accctacccc gaagattaag tattaaattt atatttagaa
    21061 tgtatcataa attattttac tttgcaaact atttttttaa aaaatatatg ttaatatata
    21121 atatagaatg tctataaact ttgaaatacc acgattgtga atataaaaat aaaaatttga
    21181 acataatcag gaaagaagtc taattaattt aaattaggag agtaatatat gaataaaaaa
    21241 cttttagaaa tactatcaaa atactatgga tatacaagtt ttagaaaagg tcaggaatct
    21301 ataataaatt ctatcttaag cgaaaaagat gtgctagcta ttatgccaac aggaggggga
    21361 aaatcaatat gttatcaaat tccagctttg attttagatg gaatgacaat agtaatttct
    21421 ccacttatat cacttatgaa agaccaagta gatgccttaa agactatggg aattagtgct
    21481 gcttttataa atagttcatt aagtagcaaa gaatttaatg aaatattaaa taatattaga
    21541 aaaaataatt ataaaatact ctatattgct ccagagagat tagatgcaca agagtttatt
    21601 gagctaataa ataataacaa tgtaagtcaa gttgcgatag atgaagcaca ttgtgtatct
    21661 caatgggggc atgattttag gttgagttat agaaggattt ctgattttat aaaaaatttg
    21721 cctaaaagac ctatagttac agcatttact gcaacagctt cagaagaggt aagaactgat
    21781 ataataaatt tactgtgttt agaaaatcca gattattata taactggatt tgatagagag
    21841 aatttatcaa taaatatagt aaaatcatct agtaaaaata agtatatttt agattatatt
    21901 caaaatcata aaaatgagtc tggaattata tatgttgcaa ctagaaagga agttgaaaat
    21961 atatataatg gtttatctaa gagaaatata tctgtatcaa aatatcatgc aggtttatct
    22021 aaaaatgaaa gaagtaaaaa tcaagaagat tttataaatg ataatgtgga tataatggta
    22081 gcaacaaatg catttggaat gggtattgat aaacctaata taagatgggt tatacattac
    22141 aatatgcctc aaagtataga aaactactat caagaaatag gtagagctgg tagggatgga
    22201 gaaaaaagtg agtgtgtact tttattttct ccaggagatg tacatataca aaaatattta
    22261 attgatattg gagttgaaaa tcctgaaaga aaattatttc agcataagaa gcttcaatac
    22321 atgattgact tagtttacag taattcatgc tatagaaaga caatattgag ttattttgga
    22381 gaaaattata tagacaattg caataattgt agcaattgtt tagtagaggg tgaaatagta
    22441 gataaaacta tagatgcaca aaaagtcatt tcatgtgtag ctagaatgaa aagaagttat
    22501 ggtgttacta ctattgtaga tgtgcttaga ggttctaaga ataaaaagat tttacagctt
    22561 ggattaaata ctttgtctac atataacatt atgagagatt attctagtga agacttaaag
    22621 aattttgtaa atacattggt ttctcatggt tttttagatt tagttgagac tctaggcaat
    22681 ggaaatagag gtagttttcc tactataaga ttaaatgaga tgtctatgaa agttttaaaa
    22741 ggtgaagtga aagtagagtt taaagaaatt gttatgacaa aatcattgga agtagaggat
    22801 gagttatata atgctttaag agaacttaga cattcaattg caagtgaaga aaggatagct
    22861 ccatatatgg tatttggaga tggtacactt agagccatgt ctagtagtta tccaattaat
    22921 aaagaagaaa tgctagatat ctctggagta ggagaaatta aataccaaaa atatggtaga
    22981 gattttgaag cagtaattaa agaatatgtc gaaaaaataa tataaataaa aaaatagatg
    23041 aaaaaaatgt cgattcaaag aacgttaata gtgatttctt agaggttaat acagataaag
    23101 ttttatatga aaaactgtta gtcattagag aagaatatgc aacaaaggaa aaattacctg
    23161 catatatgat tttagccaat aaggctttga aagaaataag tggaagatat ccacttgatg
    23221 aagaacaatt aaaagatatt tctggcatag gtggagtaaa atttgaaaaa tatggagcta
    23281 gtattttggc agaggttaat aaatatgtaa aagaaaataa tattaatgtt atttgggaga
    23341 aaaagggcaa aaggaagtta atattagatg gagaaagtag aaaaaataat gaaatagcat
    23401 tagatttgct aaatcaagga aaagatatac aaagtatcag tcaagaaata gaagtttcac
    23461 tgtcaaccat tattggatat gtgcatgact atgtaaagga tggacatagt atatcatttg
    23521 aattaggact agaaaatttt tataatgaag ataaaaagaa gatgatttta gaagtttgcg
    23581 aaaaagttgg ctatgataat ttaaataaca taaaaagaaa acttccagat tcaataaagt
    23641 atgaaaatat aagagctgtg atacttgact cttatcttga aaacttaaaa aataatatta
    23701 tagcagagta atttttaact taataaaaaa taaaatttgt attattgtgt tgaactaata
    23761 attttgctgt gttatactga atttgtaaaa ataatgaaaa attagagcta ggggcgctag
    23821 aattggctag ctgagagata aagccgtaac ttacaaaccc taataacctg atcaagataa
    23881 tgcttgcgaa gggaagcttt ttaatataat tttattatat aaataggcaa attattcaat
    23941 gttaggagaa atttgtctaa gtgtatatac gattataaga gtgctttcca aacgaggaaa
    24001 agcacttttt ttattttcaa aaactataac tttaatcttg gaggattgta atatgaacta
    24061 tacaacacaa atggatgctg ctagaaaagg cataataact aaagaaatgg aaatcgtttc
    24121 tcaaaaagaa caggtagatg taaatgaatt aagagaatta atagcaaatg gacaagttgt
    24181 tataccagct aataaaaatc ataaatcttt aagtgcagaa ggtgtaggta aaaatttaag
    24241 aacaaaaata aatgtaaatc taggaatttc aagagattgt aaggatatag aaaaagaact
    24301 tgaaaaagtt agagtagcaa tagacatgaa agctgaagct ataatggatt taagtaatta
    24361 tggcaaaact agagaattta gagaaaaggt tgttgaaatg tcacctgcaa tgataggttc
    24421 agtacctatg tatgatgcag tagaatattt ggaaaaagaa ttaaaagata taacagaaga
    24481 agagttttta aatgtaataa gacaacatgc tattgatgga gtagacttta ttactataca
    24541 tgcaggtctt acaagaagtg tttgccaaaa aataaaaaat catgagagat tgactcatat
    24601 agtatctcgt ggaggttctt tattgtttgc atggatggaa ttaaataata aggaaaatcc
    24661 tatttatact aattttgata aaatattgga tatatgtgaa gaatatgatg ttacattgag
    24721 tttaggagat gcttgtagac caggttgtat aaaagattct actgatggtg ttcaaattca
    24781 agaacttgta gtattaggtg aacttactaa aagagcatgg gaaagaaatg tacaagtaat
    24841 gatagaaggt cctggacaca tggctattga tgaaatagaa gcaaatgtag tattagaaaa
    24901 gagattatgc catggagcac ctttttatgt tcttggacca ttggttacag atatagcacc
    24961 aggttatgac catataacaa gtgcaatagg aggagcttta gcatgtgcta aaggagtaga
    25021 ttttttatgt tatgtaactc cagcagaaca tttaagattg cctaatttag atgatatgaa
    25081 agagggtata atagctgcaa agatagctgc tcatgcagga gatatagcta aaaatgttaa
    25141 aggtgctcgt gaatgggata ataagatgag taaagctaga gctgatttag actggtgtga
    25201 gatgtttaga cttgcaatag accctgaaaa agctaagaga tacagagatg aatcaactcc
    25261 tactcatgag gatagttgta ctatgtgtgg aaaaatgtgc tcaatgagaa cagttaagaa
    25321 aatattaaac aatgaggaac ttaatctaat ataatttttg gctagtatga ctggaataaa
    25381 aataagctga aaagaggagg tgatttagtg aaggttaatg ggaaggaaat tgaatttgaa
    25441 aaagatttga ctgttataga tttattaaat aaatataatt taaaaagtga tagagttgta
    25501 gttgaagtga atttagagat aattgaagaa tctaactaca atacatatgt attaaaagac
    25561 gaggacattg tagagcttat tagctttatt ggaggtggtt aaaataaaaa tatttgtaaa
    25621 tgaattatta acagatgttg aggaaaatac aactgcttat aaagttagag atagctataa
    25681 caataaatgt gatgtattgg tgttaaatgg atatccaatt aaaacagata tgaccctttg
    25741 tgaaaatgat aaattaacac ttatacaaaa aggagttaag ccatcacttg atgaattgga
    25801 aagcttgttg attgctagac atactccaaa tatacataat aaattaaaaa aagggaaagt
    25861 tgctatattg ggattggggg gacttggttc caatatagct atctctttag ctagaatagg
    25921 agtgggagaa cttttactta tagattatga tgttgtagaa ccatctaatt taaatagaca
    25981 gcagtatttt atagatgata taggaaaact aaaaactgat gccatgatag aaaatataaa
    26041 aaaaataaat ccatttataa aattaaataa acgagatatt tttttaaaca agaacaatat
    26101 ggatactatt aaggattcag atttaataat tgaggccttt gatgatgctt catgtaaagc
    26161 acaggtatgt aattatgtat taataaattt aaaagataaa tatttaatag catcatctgg
    26221 tatggcaggt tactatgatt caaatataat aactactaag aagataaaag acaggtttta
    26281 tatatgtggg gattttgtga atgaagctaa atttggtgaa gggcttatgg ctccaagggt
    26341 tgccatctgc gcaaatcata tggcaaattt ggcaacgcaa atacttatag atatgtaaat
    26401 attaaaatta gatataaaaa agattgaata ttaaaaaaat ttaacgtgga gggaaataat
    26461 atggataaat tggtacttgg tggacatgaa tttaatagca gattattggt tggaacaggt
    26521 aaatatggtt caaataatat attgcctgag gtaataaaag aaagtggaag tgaaataata
    26581 acaatggcac ttagaagagt ggatttagac aataaacaag aaaacatatt aacgtatatt
    26641 ccaaaagaga tgacaatact tccaaataca tcaggagcta ctaatgcaga agaagctgta
    26701 agaatagcta gaatatctag aaaaatggga tgtggagatt ttataaaaat agaagtaata
    26761 tcagatacta gatatttact tcctgataat gaagaaacta taaaagctac aaagatactt
    26821 gctgatgagg ggtttatagt tcttccatat atgacaccag acctttatgc tggaagaaga
    26881 cttatagaag ctaatgcagc ggcagttatg ccacttggag ctccaattgg ttcaaataga
    26941 ggtcttcaaa tgaaagaaat gataagaata atgatagatg agttggatat accaataata
    27001 gtagatgcag gtattggaaa accatctcaa gctatggagg ctatggaaat gggagctgat
    27061 gcagtacttg tcaacactgc tatagcttca gcaggagacc ctgtacaaat ggctagagca
    27121 tttaaattag ctgtagaagg tggaagagaa gcatatattg caaaaacagg aaatgtgtca
    27181 gaatttgcaa atgcatcttc tcctttaaca ggttttttag gcaatttata gaaagtggtg
    27241 attggtgatg agtttttatg atgtaataga aaaatatagg gattttgatt ttgatggata
    27301 tttaaataat gttactgata atgatgtttt aagaagttta tcaaaagaca agcttgaaga
    27361 ttttgatata ttaaatttgc tttctaaaac tgcagtaaaa catttagaag atatggcaca
    27421 aaaagcacat aaattaagtg ttcaatactt tggtaaaaca gtatgtttat atacaccaat
    27481 gtatatagca aattattgtg ttaatcaatg tgtttattgt agctataata taaagagtgg
    27541 aataaaaaga aaaaaattaa ctatggatga aattagagaa gagggagaag caatttcaaa
    27601 agaaggtttc aaacatctac ttgttttgac tggagaaagt agttttcact caagtgtaga
    27661 atatataggt gaggctatag aaatattaag agagaaattt ccctctatag gtatagaagt
    27721 ttatccaatg gaagtagaag aatataagta cattgtagat aaaggtgttg aaggattaac
    27781 agtatatcaa gaaacttatg acgaggaaat atataagaga gtacatataa aaggcccaaa
    27841 atcaaattat aaatttagac tagatgctcc tgaaagaggg gcaaaagcag gtatgaggac
    27901 tttatcaata ggtgcacttt tagggctaaa tgattttaga aaagaaactt tttttacaat
    27961 attgcatggg aaatacttaa aaacgaaata tcctcatatt gagctttcat attcaacacc
    28021 aagaatgaga ccttttaaag ggtgttttga ggaattggta gatataagtg atactgattt
    28081 agttcaagct atggtatgta tgagattatt tgaccctcat gcagctataa atatatccac
    28141 tagagaaaat ttagaaatga gaagtcatat aataccttta ggagtaacta aattgagtgc
    28201 aggtgtatct actgatgtag gaggtcattc tcaagatgaa catgatactg ctcaatttaa
    28261 gataaatgat gaaagtactg ttaaagatgt ggagaaaatg ctaaactcaa ttggttatca
    28321 gcatgtgttt aaagattggg aaagatttta aatgtatctg ataacaaaca gaaaattatg
    28381 tagtgaggaa cgatatttag aagttattaa agagtctata ttgagtggtg tagaaaatat
    28441 aataataaga gaaaaggatt tagaatatca agagctaaga aaattatata tgaaaataaa
    28501 aactaaaatc aattgtatag attttcaaga gcaaatatca gatgaaagtt tgaaaactaa
    28561 tataaatcaa aaagagtgta gaaataaatt caaggttaat tttataataa atagtaacat
    28621 tgaatttttt gaaaaagtgg attgtcaagg tatccatctt ccttttaaat tattcttgaa
    28681 tttaattgaa aataagtata attttaatga aaataaaata ctaggattat ctttacataa
    28741 agtagaggaa gtagattatt tagaaaaatt aataagaaat caaaatataa aaatagatta
    28801 cataacatta tcacatatat atgaaactaa atgtaaagaa ggattaaatc caaaaggaat
    28861 tgagctttta aaagaagcta aaaagattac tgatattaaa ataattgcac ttgggggaat
    28921 tttaccatct aatgtcaagg aaacattaaa atactgtgat gattttgcta taatgtcaac
    28981 tataatgagg tctaaagata ttaaaaaaac catatcaaat tataatgaaa agttgaatta
    29041 gtaaatatag tatattgaat ttttaactta aataaaaata aaaaagttat cttttaaatt
    29101 ttaaaaggta gcttttttat tttatatctt tgctaataaa aaattgtaaa aagggaaaac
    29161 tttagtcaga gagattaaac aatatcctag aaagatttaa aatttagtaa agtatggagg
    29221 acattataat gactgacagt aaaaagaaac gatttattat accacacact tttacaataa
    29281 tatttttact aatagtcttg atggcaatat tgacttggat agtaccaagt ggtgaattta
    29341 gtagagaaga tgtagaaggt agaatggtag ttgtaccagg gacttatcaa agtgtagaaa
    29401 gtaatcctca aggatttgca gatgttttta aagcaccaat agaaggcttt atcgattcag
    29461 ctgaagttgt aggttttgtt ttaatagttg gaggtgcttt tggtattgta aataggactg
    29521 gagctataga agcaggaata gcttcggtta tctctaagtt taagggaaaa gaacttctta
    29581 taattccaat atcaatgatt ttatttggac ttggaggtac aacatttggg atggcagaag
    29641 agacattacc attttatatg atatttgttc cactcatgac atccttggga tatgattctc
    29701 taacagcaat agcaattgta tttataggag cagcagcagg agccgccgct tcaacaataa
    29761 atccattttc agttggtatt gcacaggcac tagctcaact tgctcctggt tcaggagtaa
    29821 cttacagagt agttattttt atagcatata tactaataag tatagcattt gttatgtact
    29881 atgctaagaa ggtaaaggaa aatcctgaaa gttcaatagt atatgaaata gataaaaata
    29941 ataaatcaat acttaatcaa acttcaacag aaataaaaga atttactttt aaagaagtat
    30001 ctgttcttgc tatatttgga atagggatgg caataatgat ttggggtgtt ttaagtcaag
    30061 gatggtacat tccagaaata tcaatgtgtt ttgctgcaat aggtgtgctt tctggattgg
    30121 ttggtagatt atctcaaatt gaaatctcag aaagttttat agatggagca aaagatttag
    30181 catctgcagc tcttgctata gctttggctc gtggaatcgt aataattgct caaaatggat
    30241 ttataataga tactatatta aattccgcag cagcctttct aggtgggctt ccaaaggtta
    30301 tatttgtata tttatcattt ttcttggaag ctgcattggc atttttaatt ccatcatctt
    30361 caggtcttgc ttcacttaca gtacctgtac ttgctcctct tggtgatttg gttaatatat
    30421 cggctcaagt gatagtaacc gcttatcaat ttggtgtggg tcttacaaac tttataactc
    30481 caacttcagg agtactgatg ggagcacttg cagtagccca tattccattt tcaaaatggg
    30541 taagatttat aatgcctatc ttgttgattt tgacagctgt atctctagtc tttttaacaa
    30601 taggtattta tatagggttt taattaagtg tagttttatt taagatattt agtaatggta
    30661 taaaatgata atatgttttc aataaattgc cactaattta attagtggta atttgttttt
    30721 tgtatttaaa aataaatata tttacttgta ttttgaatta atatctactt agacattgtt
    30781 ttaattgtta tattttcatt taattaagca ttgatttatc attttttata tttcatataa
    30841 caatgcaaaa aataattttt atatagtaaa tgacaaaagt ttacaataaa tgtatatttt
    30901 cttactcatt ccttaaaatt actcatttag tttaataact ctcatttcat atgtaaagtt
    30961 ggtaaaaaaa ttaaaattaa aagtaaaaaa ctaaataaat taatgttaat ttgaaatata
    31021 tatgaaggtt tttggagaat acttacttat gataggaaaa aatgttaaaa taatagtgta
    31081 atcaaatata aaggactgaa ataattaaca gttattaaat attaggagtg taaaaattta
    31141 tgggattaaa agaatttgat tttattgaag agtctattga tatgttgagg acaatgtcac
    31201 caacattaga aactatatca gatgaaatag aagagtattt tgaaaatatc ttggatgaaa
    31261 agaatcaaga atatataaat gtaacctcta gaataaaatc agaatcaagt ttaagagaaa
    31321 agataattag aaatagatat ttaaaaaaat atggagaagc aagtaatctt attcataatg
    31381 tttctgactt aataggccta agaattgagt gtagatttat tgaggatgaa aacaaaatat
    31441 atagactttt gagaagatat tttaataaaa cagatgacaa aataaactat tataataaag
    31501 agaataagaa tattaaatta aaactatctg agagacaacc caataagcaa aaaaatggat
    31561 ttgaaatata taaaatagat ggagtttttg cgtatttaga tagagaagta aaatttgaac
    31621 ttcaaattaa atctcttgta aatgtgtttt ggagtgaaat agaacataaa ataatttata
    31681 aaaacaatac atatttactt gctgataaat ttataaagga tatgatggac tctataaaga
    31741 ataatcttac tatgatagat aatcagcttt taagtattta taaaaacttt cattcaggga
    31801 agtcttttaa tatgaaagta agtaaaaaag aaattgaaaa attatttgcg aaactagtct
    31861 atgacgcttt ttctgaaaag atgaataaaa gtataggttt tgttgtagat tttaaaaaac
    31921 catgtgagac tatattgaac tattctttta ataaacaaga tataagtgat gaagttttag
    31981 gtaattttat gcttgatgag tttgcaagat taaatgaaat tgtaaacaag gatatagatt
    32041 ttaatgagca aatagaattt gaaagagaac caacatttga tgataagttt tgtaaagacc
    32101 taggaaatca ttttagaagt agattaaaca cagaatttcc atggaaccta ttttttagaa
    32161 tattatttga aatagagcca tataacaata ccaaagactt tgaaaacttt gtagagttta
    32221 taaaagaaaa tgtgctacta gaagaaaata gggagcaatt attatgccaa tttgaagaag
    32281 atagcaaact tataattgaa gatatatatg attgtatatt taatagtata agtgagattg
    32341 atagtgttga aattttatat aactataatc ttgaaaaaat aaactttaca tcaagtgaaa
    32401 tcataaattg tatatgtaga gaatatgagt gttatggtga atacttagaa gaaaaagaaa
    32461 agcttatgaa tacatttaaa gaaaagatta ttcaaatctt tgagtaatag gtcaatgctg
    32521 aagtggcgct aatgcgccat ttttcaaata tatatgaatt aagtaaaatt attttggagg
    32581 aggcgatttg tatagagaaa acaggtgtaa ttttggcagg gggaagaaat tctagaatgg
    32641 gtagagataa agccttttta gagttacatg ataagctttt tattgaaatt gcaatagaag
    32701 catttaaaaa ctttgatgag ttgataataa tatctaataa tgaagaatta tattctaaat
    32761 acgatattaa agtttataat gatatagtaa aagatgttgg tccaattgga ggaatatata
    32821 ctgctcttga atatgcaaag tatgatatag ttacaattgc atgtgatatg ccatatctaa
    32881 ataatcaaat agtcgaaaga atagctaata aaatgaatga taaaagtgtg atttcagtga
    32941 caaatggaaa gttacaacca ttgtgttctg gatataaaaa gagtataata aataaagtat
    33001 ctttatgtat aagggaaaat gatttaaagc tcagaagctt tatagataaa atagataaaa
    33061 gttatatata ctttgatgaa gaagatttat ttttaaatgt aaacactgtt gatgagtatg
    33121 aaaaattaac taaagtttaa ttagctatgg ttgtaaaata gagtaggatt tatatggagg
    33181 agagtttatg aatttactta aatgcgattc ttgggcagta atattaaata atagcgataa
    33241 agaaagtaaa gcatataaaa tattagatga acttaaaagg aatatgtaca aagttgttgc
    33301 tatagatgaa gagaaaaaac ctattgaagg aatagatgtg tatgaatgtt taaaagatgt
    33361 tccacacaat attgatgttg tagctattat tgataaacaa tctaagatgg atgtgatttt
    33421 agaagaagta gagcttttgg atatacaaaa tatgtggttt gaaaaaggaa gctttagtga
    33481 aatgatgatt aaaaagacta aagatttaaa attaaatata gaatataatt taagtttata
    33541 tgatgagttg actagataaa attatatatc ttaataataa aaatataatt agttaataaa
    33601 acatattatt ttatagtata gattggttaa taaaagtaca gattggttaa tgaaacatta
    33661 aattacaata tttgttaaaa aaattgtgag ttacatgata taatgtatta aataggatac
    33721 taaggagttg atatattatg ttaatagtaa ctacagaaaa agtagaaggt aaaaagatat
    33781 caaaggtttt aggattagtg agaggaagta caataagagc aaaacatgtt ggaaaagata
    33841 taggagcaag ttttaagaat cttgttggag gagaacttac tggatataat gaaatgctca
    33901 ctgaagcaag acaaattgcc ataggtagaa tggttgaaga tgcagaagct aaaggtgcaa
    33961 acgcagtaat agcatttaga ctgtcttcag cttcagttat gcaaggggca gcagaaatgc
    34021 ttgcttatgg aacagcagtt gttttagaag atgataatag tattcttgaa aaataaaata
    34081 taaatagtta aaaagaggca tcttttaaaa attttatgga tgcctttttt tatttgaaaa
    34141 aactaatttg agaagaaatt ttaactatta tgacagtaaa attagggtga tattatataa
    34201 ataagtaaaa ttatattata taatgtatgg tatagacttt aaaaatttta tttgtagaga
    34261 aaaatatgga caatgttata ataattatag aaattaaaag tacttacatt cattatgtat
    34321 gtgacatgta taattagttt tatatttaat acagttttga agtaatatct tcattttgag
    34381 aacgttggaa taataaaaaa tgtattatta taggtgatat aaataaaaaa taaatataat
    34441 atgttatatt gctgatagaa aagagaataa aagaaaatag gttttattaa aagagatttt
    34501 tatttatagg acttaatcaa tgtatagagg ggtgagaaca tatgtttaat gttgcaatta
    34561 taacacttag tgataaagga tatgaaggaa aaagagaaga tataacagga aaaaagttaa
    34621 ctgaatttgt tgaaaataca ggagcttata aagttactga atatgtactt ataaaagatg
    34681 ataaggaaat gctaaaggaa aatattataa gactttgtaa tagtaataaa atagatttaa
    34741 ttcttacaaa tggaggtact ggtttttcaa aaagagatat aaccccagaa gcaactaagg
    34801 aagtgttgga aaaagaaata ccaggtctga gcgaatatat gagaatgaaa tccacagaaa
    34861 ttactaaaaa ggccatacta agtaggggtg taagtggaat aagaaataat tctataataa
    34921 taaatttacc tggaagtcct aaaggtgcag ttgaaaatct aagttttata attgatgttt
    34981 tagaccatgg catagaagtt ttaagaggag aagctacaga atgtgccact aaaaaaagtg
    35041 aatagtatat aatctagata gataaaaaat agacaaagag gtagataaag tatgattgat
    35101 aagtatggaa gaaaaataga ctaccttcgt atttcactta cagataagtg taatttaagg
    35161 tgtgtttatt gtatgccacc agatgtaaaa tttgataaaa attatataaa tgaaaatcta
    35221 agctttgaag actacaagtt tattataaag gctatggctg aacaaggtat aacaaaaatt
    35281 agatttactg gaggagaacc tcttttatat cctaaattaa atgagcttat aaaatttaca
    35341 aaagaagaat gtggaataaa taacatagga atgaccacta atgctattgg gctttctgat
    35401 agaatctttg agcttgaaaa atgtggctta aataaagtta atataagttt agattctttg
    35461 aaggaatata agtataaatc cgtaacaaga ggaggaaact taaaagatgt cttaaaatcg
    35521 attgaatctt gtttaaactt aggtataaaa gtaaagttaa attgcgttgc tataagtggg
    35581 tttaatgatg atgagcttaa agattttatg tttttaacag ttaaatcacc aattgatgta
    35641 agatttatag agcttatgcc attaggtgag gctaatagag tttataagaa gggttatttt
    35701 aatgtaaaag aaatgattga aaaaatatat ggtttatata aagtcaaaaa tgatgaaaaa
    35761 agtacagcag aatattatat gtttaaagga gctaaaggaa gaataggaat tataactcca
    35821 ttaagctgtt cattttgtaa cacttgtaat cgtattagac ttacttctag tggtgcaata
    35881 aaattatgtc tacattcaaa agaagaaata gatataaaac catttttaca taaaccattg
    35941 ttatttagag aatctatgaa agatataata aaacaaaaac cagaaaaaca tcatttaata
    36001 gaaaataaat gtagtgatac aaataggcgt atgtatgaaa ttggaggata agtaatgtta
    36061 acacatataa atgaaaaagg gtatgccaag atggtggaca taagtgaaaa agatgatact
    36121 aaaagaacgg ccgtagctac agctacaatt actcttagta aggaagcatt agaaaaagta
    36181 aaaaatggtc agataaaaaa aggagacgta ttgtctgtag ctcaagttgc aggtataatg
    36241 ggagctaaaa atacatcttc aaacatacca atgtgccatc agattaaatt agttggatgt
    36301 gatttagaat ttgatgtaga tgattctatg tctgctatta agatatacgc aacctgtaaa
    36361 tctcttggga aaactggagt agaaatggaa gctttaaatg catgttcttt agcagcactt
    36421 acaatttatg atatgtgtaa agcagttgat aaaaaaatgg ttataagtaa tatccatctt
    36481 gtagagaaga ctggtggaaa atcaggagat tttaagttcg agtaaattaa taatatattg
    36541 ggttaaaatt tggaggaaat atggcgaagg tagtgtcaat aaatataagt gagaaaaaag
    36601 gtgtaattaa agttcctgta caatgtgcag agctaaaaat aaatcatggg atagttggag
    36661 atgcacatgc aggaaactgg catcgtcaga taagcttact tggaaaagaa agcatagata
    36721 aaatgaaaat gaaaggcttt gaccaattaa aatatggaga ttttgctgaa aatgtaacta
    36781 cagaagggat agaagttttt tccctgcctg ttggtacaaa gttaagaata gggcaatgtg
    36841 aagttgaagt aactcaaata gggaaaaaat gtcacaatgg atgcgaaatt aagaaattga
    36901 caggtgactg tgtgatgcca cgagaaggaa tttttgtaaa ggttctaaaa gaaggaatta
    36961 taaatgtgaa tgattcaata gatgtaatct aatgaagtca ctattaaaat ataattataa
    37021 gtaaatacta ttaagattta aatatatatt ttaagagaga attaatttta ataaaatatt
    37081 ttagttataa attggaatat tggttatata aagctatgaa tgtaaaatca tagctttttg
    37141 tttttgaatt ttaaaatata atattttatt tatatatatt aaattatatt ttatataaaa
    37201 tcaattgttt aaatatatat tatttatact gtaaatttat agataataaa gtataaaaaa
    37261 aatatatttt gatgaaattt aaaaaaatac attatataaa tatagtaata gtatgaaaat
    37321 ttacatataa aactaaaaaa tgcaagtaat tgacttttta agttgacaaa atattgcata
    37381 tatattatga tataaatcat aaattaacta caaatcaata ttatatattc gtagaagaag
    37441 aaagtagcat aaaggaattt tacagagagt tagatttggc tgagaactaa tattggaatt
    37501 tatgtgaaaa ggactttgga gaatactatt gtaattatgt tagaatagtg gtttaattcg
    37561 ccttaagaaa atgagtgagt aaaattaatt tttactaata agagtggtaa cacggattta
    37621 tattcgtctc ttggattttt ccaagaggcg ttttttatta taaacttatt tcagaaaatt
    37681 taaaactata tattaaatga aaattataaa aatatgatag ttaataaatt tataaattag
    37741 aaaattgtaa aatatgctaa ggtcagtagt tttacgattt aaatggaggg atgaaaatgg
    37801 aagaagaaaa aattaactat gctctggaac agattccaga gaatcaaaaa cgtggttggg
    37861 tagctatgtt ttcagtttta gtagcaatag gtgtggattt atcatctgtt attttaggcg
    37921 ctgagttggc acagagtatg ccaatgaaac aagcaatatt gtcagtaatt gttggttcat
    37981 ttttttctgc aattttgtat actacatgct ctttggttgg ctcatctaca agtttatcta
    38041 catctatgat aacaaagtat gtatttggag aagctggagc aaaaatattt tctctagtta
    38101 taggggtttc tctattaggt tggtttggag ttcaagtagg tttctttgct caaaatgcac
    38161 agataataat aaaagatata tttaatttag atgtaagtat gcagatttta agcctaatag
    38221 gtggtctctt gatgatgagt actgcaattt atggatataa ggcaatggag aagttgagtg
    38281 tgtattcagt accattttta ttagttctta tgatgctaac tatattctta gcttttagag
    38341 caaatggaat ttcagttgat gataatatga aatctactat gacatttgca ggtggagttt
    38401 ctctgtcaat gagtataata atagttggtg caattgtatc tcctgatata agtcgttggg
    38461 ctaaaagtag aagagactgt gctctttcaa gctttttggg aatacaattt ggaaatgctt
    38521 ttatgataat agtttcgata gtattagtaa aatgtatggg aacttcagat attatgagaa
    38581 tttttattac attaggaatt gctataccag gtataatagt acttacatta gcacaatgga
    38641 ctacaaatac aagtaatgta tattcagcat cattaagtat agctcttgta cttaagaagg
    38701 ctcctgagaa agtattaact atagtccttg gtattatagc aacattattg gctgtatttg
    38761 gaatatatga aggttttata ggatttttaa atttacttgg aattgtaatt gcacctgttg
    38821 gtggtgtata tacagcagaa tattacatag taaagcaaga acttaaaggc tttgataaag
    38881 gagtattata taaaccaata gttaaacgtt caatagtgtc ttggataatt ggtatattaa
    38941 taacttacct ttcaacatat ggatttataa ctcttactac tatagcacct ttagatggtt
    39001 ttatagcagg gtttgtagta caatctatta ttggaaaagt gctttgttca acaaaaaata
    39061 agcaagaaat agacaaagca gtataaagaa atttgtttat aaagtataaa ataaattaga
    39121 aatatgagga gaaagtaaca tgagattttt aacagaagaa agtgtagata aaatagcagt
    39181 tggtgcagca gtgcttggaa ctggaggtgg aggtgaccca tatgtaggta aattggttgc
    39241 aaaacaagcc attaaaaagt atggtcctgt aaaagtaata agtttagatg aacttgatga
    39301 tgatgctttg gtagttccag tatctggtat gggttctcct gtaattacaa tagaaaaatt
    39361 actttcagaa gttgagttaa ctaccccttt agaaataatg gagaaattat taaatagaaa
    39421 agtggatgtt ataattccta tagaaatagg agggataaac tcacttatgc ctatagcagt
    39481 tgctgctaaa aaaggacttc ctatcttaga tgcagattct atgggtagag ctttccctga
    39541 agctcaaatg gtaacttttt atcttgaagg atatgaggca tctcctgccg taatgtctga
    39601 tgaaaaaggt aattctgcca tcctttatcc tgtggatgga atttggtcag aaagattagc
    39661 tagaacttta actgtagaaa tgggaggaag ttcttctatt agtgactata atttaagtgg
    39721 agctcaagtt aaaaaagctg ctataggtaa tactcttaca atagctgaaa ctataggagg
    39781 gtttttactt gaaaataaat ctgattcaaa aggtgctgtt gagaatatac taaaagaatt
    39841 gcgtggatac aagttatttg aaggaaaagt aattgatatt aagagagagt tgaaaggtgg
    39901 atttactaga ggacatgcct tttttgctgg gattaatgag tatgatggag agtattctat
    39961 attattccaa aatgagaatt taattgccaa aaaaggtgat acaccacttt gtattacacc
    40021 agatttaata gcagtattag atttagaaac tggatttcca ataactacag agagaataaa
    40081 atatggctct cgtgttatgg tagtagcttt tccttgtaat gaaaaatgga gaacagaaaa
    40141 aggaattgag acagttggac ctggatactt tggatatgat gtggaatata aaacagtaga
    40201 agaattacaa ggaaaataaa agtttaggag gaaattttga tgtatcgtgt aggaattgac
    40261 gttggaggaa caaatacaga tgcttgtata ctggatgggg aattaaaagt aattcattca
    40321 gttaaggtgg caactacaaa agatgttgaa actggtgttt acaatgcctt aaaaaaagtt
    40381 atagatgaaa gtaagattga ccattcatta ataaaatatg ctatgcttgg tacaactcat
    40441 tgtacaaatg ctattgtaga aagaaaaaga cttaataatg tggctatgat aagaatagga
    40501 aagccagcca ctactgccat tcttccattt atagattggc cagaagactt aagagaaaaa
    40561 atagaattag attcaatatt ggtatctggt ggatatgagt ttgattaaaa aatagctacg
    40621 cacactcgtg actttagtca tgagttagta gctaaaatag tcagcatata gggaaaccta
    40681 tatgtagaga taggata
//
LOCUS       DAEPIN010000027        40671 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.027, whole genome shotgun sequence.
ACCESSION   DAEPIN010000027 DAEPIN010000000
VERSION     DAEPIN010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 40671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40671
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.027"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            166..1002
                     /locus_tag="KR250_001696"
     CDS             166..1002
                     /locus_tag="KR250_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBH0067632.1"
                     /translation="MNIKKIFNNNVVVSSLEDGTEIIVTGAGVGFKKKVGDLIDENLI
                     SKKYFVQDDQRDKYNQILNKTSIEYFKISEEIIEKANEVLNTQVNDSIILALTSHIEF
                     AVQREKQGIKLPNLILNETKQLYREEFEFGLWAIDEIEKRIGIKLPEDEAGYIAIHII
                     NGLENSQKDEGINILEFSKQVIQIIEEVHGFKLDVNSLNYTRLITHLKFFVQRILRKE
                     TYNDSDIEDMYKLVNKNYNKPKVCTEEIAALVLDRFEYKISKEEELYLMIHINKITNS
                     GC"
     gene            1355..2932
                     /locus_tag="KR250_001697"
     CDS             1355..2932
                     /locus_tag="KR250_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBH0067633.1"
                     /translation="MKKNKLGLWNFFQMLGKTFMFPIALLSVSGMMLGLGAGFTDPKM
                     IEMVPFLGNEYVNLVLNFMLTIGLFAFNNLGALFAMAIPLGLLKKEKEFGAFSGLVGF
                     IAMHIGTNFYLTRADLLVPAEQMSTNGQAMIMGIQTYNTSVLGGIVAGLIVYAIYDKV
                     SNLKIPESLGFYSGPRLVPIVSLIVMSIVGLLIPIVWPPFFNAFQSLGRWISSAGPLG
                     YFSYAVAERVTIPFGLNHLVTSVFRFTPIGGTAVIDGETYFGTLNMFMAYVENNQIIP
                     LDLAGKMEQGKLMIQYGLAGAALAIYRTAKPENRKALKGLLISGVLTVIIGGISEPIE
                     FLFLFISPALFAFHTFMNGIANMVLPYLGVLMGFTGDLIAFISFGVLRGTATGWPIAV
                     LVAVLYFAIYYFVFKWAIIKFNIKTPGREDKEIKVSEGNGSDFKNLSTYKGQQMISAL
                     GGQSNILSLDNCVTRLRLKLQDVSLINEDAIKEAGGIAVVKLDEHTIQVIIGTQVYSL
                     RKQMDKAMEGHLEACND"
     gene            2925..4109
                     /locus_tag="KR250_001698"
     CDS             2925..4109
                     /locus_tag="KR250_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HBH0067634.1"
                     /translation="MINFDKKVDRLGTYCTQWDYVEDRFGKKDLLPFTISDMDLEFPF
                     EITEVLQERLNHRVLGYSRWKHDDFRNSVTNWYIKRFNCSVDSNWIYYSPSVMYSISK
                     LIEMLSNEGDGILINTPAYNAFYDVIINNNRNIIKSHLVNRNGFYSIDFNDFEKKCKK
                     SKIFILCNPHNPTGRVWSELELVKMIGICKNNDVKIISDDIHMDIVYDNRMTPILNVA
                     KDYLDSVFICTSASKSFNIPALTGSYVIIPNEGIRNKFENITRYRDFVNSPAILAILA
                     TMVCYDDCEYWLDELLKYLKENLKYTIDYIKDNLEPLKLVMPEGCYFAWIDFSELKIS
                     GEKFQKLLMDIGEVAIMSGSVYGEEGEFYLRLNVACSREKLEDGLNRIKKTMDYIAKR
                     IL"
     gene            4127..4726
                     /locus_tag="KR250_001699"
     CDS             4127..4726
                     /locus_tag="KR250_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBH0067635.1"
                     /translation="MGYLKTMVDVMTTEMNAIRCLIDEAGIEYESIVSEIANCEGKVI
                     FMGVGKSAHIGKKLAATFASTGTPSFFVHATEAVHGDLGMIESKDITILISNSGNSME
                     VVNCIKYIKAIGSKTIAFTSNRNSVLAKECDYALIYPAKDEADHLNLAPTTSSTITLV
                     LGDSIACALSKSSNFGSSDFYKYHPGGSLGEKLKTANNG"
     gene            4750..5220
                     /locus_tag="KR250_001700"
     CDS             4750..5220
                     /locus_tag="KR250_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBH0067636.1"
                     /translation="MFKMFKKKSIKSPISGKVIELSKIQDGVFSQKIMGEGVAIDSTG
                     DIVYAPINGKIVVVAETKHAFVIELENGMELLIHVGLDTVNLKGEGFEVLVSMGEQVK
                     EGTPMLKINRPLIESKGISLITPVTITNHSEYNMNTCNVGNSVEGGKDTVIEFK"
     gene            5592..6338
                     /locus_tag="KR250_001701"
     CDS             5592..6338
                     /locus_tag="KR250_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HBH0067637.1"
                     /translation="MKIENLSIDDIKRGYIFNDDLNCYSCIKCTKTYEVGEIYKIGNR
                     FFEASKAIELHMELEHSDYLEQLLNSDSKYNTLTENQKQLFQLFISDLPDKEIAKQVG
                     VSTSTIRHQKFIFREKAKQAKLYLALYESVFEDKSNKSSTIIPIHEHARMVDERYVVT
                     EEERKHILDTSFESMNPLKLKVFSSKEKKKVVILSKILEQFERGKKYSEKEVNQILES
                     IYDDFATIRRYLIEYGFMSRNKECTKYWLT"
     gene            6399..7073
                     /locus_tag="KR250_001702"
     CDS             6399..7073
                     /locus_tag="KR250_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase"
                     /protein_id="HBH0067638.1"
                     /translation="MNDELKSILYNKGVDIVRFVDISEFPINQTQGFSKAILFCIGLS
                     KKFITDIYNNLPTDSDEFLEKEEKVEELADWISKYIQNKGYRAYSQSEKNNLEHGYFE
                     KAYINPEMQSGISPLPHKTIANISGIGFMGKNNLFVTEEYGCAFSMCTVLTDAPISVE
                     RYPLIDSKCMDCNVCVENCPAKAIHGNEWTLPGKRESIIDVSKCFCVLRCMMSCPWSL
                     RYANQK"
     gene            7442..8788
                     /locus_tag="KR250_001703"
     CDS             7442..8788
                     /locus_tag="KR250_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 55 protein"
                     /protein_id="HBH0067639.1"
                     /translation="MEIETKTPDWINVLELNSKIDITGKYDVSNMIQNIVSDKSLEGS
                     IIYFPKGNYLFEKGIKISQQITLQGDSYYGGGNQVSNLDKKQSIIGTTNFITRGVSNM
                     SIITLSGTSQCIKNINFYYDSHDIEKIPPKNVSAITEYGETQGLSHFEHLFISGFSGI
                     GIEIPYYSTGNDITVSSCGLGMRLGEKSMLSSSKIYECKNGMEITTGVSLNNVRIEEI
                     KEIGINNKGFGFNLIMNLMVDQCGYCGFMFEKMCYSQITARITRCGQYYKSVDYNAYE
                     NMEDRKEEAYSIFYGDILENCNIVLMNSNIDTLENDSLDEHKIYVIKANETNNVMLTC
                     YAEADNFIQCAKGNLLLENGENTYKFYNGEICSVGGVQISDIDEKDLIKIIENTLYID
                     DKGKYLMVPRLKKNTVIYSVLDSTEEINKLHGGIWEQIDVKVLAGESMYYYKKIKD"
     gene            8815..10215
                     /locus_tag="KR250_001704"
     CDS             8815..10215
                     /locus_tag="KR250_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0067640.1"
                     /translation="MEESIIIKDISGWINVLDYGICNDETKDVSFKLQEIMDAVPEDS
                     IIYFPKGKYLFESGVKIKKRLTLCGDSYILANVPKNTAGVTQFTFKGKKNPKSENIAI
                     ITAKSVRHCIKSICFYSDSCDRDMTSNHEPPTDGKPRFHHNVIINYRNISAIVCEDKQ
                     GESGHYKNLSFSGFSGIALDMPNNSTANDITIFTSGLGISTGENSTIANSKVWGCVDG
                     MIISTGTFINGMRVEEIKKVGIKNIGRGLNFITNITIDQCGYCGVWFDSISNVQISGN
                     ITRCGQYYYDVSYDNYLMLDNRVKEAYSLFYGNLLESSNIVLSNTNKDNWEDFKEDKH
                     KVYLVEALETKNVLLTCNIDATDWIVKSEKGNLTYNNMRQTVIFYDGKLSSINGIGIS
                     DEDYGDKVKIKKGNIYINSKDKDIKMHKPEVGDIMSTIIKDKSILSAHYGGTWEKIGE
                     KIEFGITVHYYKLIEK"
     gene            10425..11861
                     /locus_tag="KR250_001705"
     CDS             10425..11861
                     /locus_tag="KR250_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 55 protein"
                     /protein_id="HBH0067641.1"
                     /translation="MEGRTGIKDGRWINVVERGIKNDGSYNVGPALQDIIDEIETDFC
                     VLYFPKGKYLFTSGVKINKEIVLQGDSNSSTGTTQFITRGVENMSIITLTGKKQCIKN
                     INFYSDSCEIQVNKEPPTKGNPKYHYEMIINKNEKNVEMNNVSAILYDNSKNMIKGLG
                     HYENIYISGFSGTGIRIPYYSIVNDITVSSCGLGIDTGIDTIISNSRISKCQNGMRIR
                     TGTSINNVRIEKIQKVGLISSQVKGYEGYGSYKINNITIDQCGYCGFSFDSMKDSYFS
                     GIIRRCGQYYYNTDYDTYLSIKDRVEEAYSLFYGNYFTECNIDLVSDNKDNWDDGLEY
                     KHKVYVFKTLQIPKLTLRCNIDAYDYIVKSVHGGGNLMLKNNFNNYIFIDSSNPEVIN
                     GINFNYQKNGNLLRLNNGKLSYKNGKEETAIFRPNYGLIVASTDSECEQIETSYGGKW
                     EKLGSEVIGEETVYYYKHHEPDKTNQIQ"
     gene            12205..13149
                     /locus_tag="KR250_001706"
     CDS             12205..13149
                     /locus_tag="KR250_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1848 domain-containing protein"
                     /protein_id="HBH0067642.1"
                     /translation="MIVSVSRRTDIPAFYSKWFFNRLKEGFVYVINPMNPKQVSKIEL
                     NPHTVDCFVFWTKDVTPMLYDLNKLKDYKYYFHYTITSYGKEVETCILDKRKVIDSFK
                     ELSRKIGKERVILRYDPIFLSEKYTVDYHIKAFASLCNQLDGFTEKCIISFIDLYKKT
                     KFNTKALHIKPIEITEIETLSKEISKIAHKHGITLEMCSEEYNLSKFGIGKSKCIDDR
                     LISKIIGCEVEVKKDSTQRDICGCVKSVDIGQYNTCRHYCLYCYANFNYAKVEENCRL
                     YKEDNKLLIDTLRDDAKISIRDMKTIKVDKNKQISIFD"
     gene            13318..14250
                     /locus_tag="KR250_001707"
     CDS             13318..14250
                     /locus_tag="KR250_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HBH0067643.1"
                     /translation="MNLLKKYPIPIASLILSIFTLGNLLQSYSESLRNVIGFIGFILY
                     AIYIVKIILLRKGVKEQLENTLISSTFPTITMATMIFATYIKPLSLELSIMIWSIGIV
                     GHLVLIVLFSKKFLVKFSIKTVFPSWYIVYVGIVAASVTAPAFNQLLIGKIAFWIGFV
                     SYIVLIPVILKRVWIIKEMPEPSLPSIAILAAPGSLLLSGYINSFSDKNNVVLYLLFI
                     LSIVFYIIVLGYLPKLLRLPFYPSFAAFTFPMAISAMGMKLMNGYLTKSGHFISWISY
                     ASKLEEIIATVIILYVLVLFIIYLSKSETTSKAK"
     gene            14355..14822
                     /locus_tag="KR250_001708"
     CDS             14355..14822
                     /locus_tag="KR250_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C_GCAxxG_C_C family protein"
                     /protein_id="HBH0067644.1"
                     /translation="MKTKEEYLKEVRNISEEYFRKGEFFCSEAVLQTINDALGQPLSP
                     EITKLASGFPIGLGKAQCLCGAISGGEMALGIVYGRVHGEAMNPKMFEHAKGLHDYIK
                     KEYGATCCRAITKKWDGDNFMSPERKEHCIKITGQVTEWVADKLIEDEKLNIK"
     gene            complement(15066..15920)
                     /locus_tag="KR250_001709"
     CDS             complement(15066..15920)
                     /locus_tag="KR250_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009912760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HBH0067645.1"
                     /translation="MLTIIIALLPVMGWGLMPIVANLKKSSPHEQLLGTSISAFIFST
                     IITLVIHPEITFFSFCISMISGIFWSLGQLMQFKAIQIASVSKVMPISNGSQLTFTTL
                     LAVILFNEWTSSKMLLIGSLSILCIILGILLTNYQTKKSTDKNMLKCVGVVLLSSLFL
                     SLYVVTNQIFLIEGYRIILPQSVGMLITSSIIVKYFSKEIVYRENVLFNLITGILWSI
                     ANLGMFITTNTLGVTISFSISQSCVIVATLGGIIFFKEKKNKKEWLAIFIGIILIMSG
                     VFMLSIIK"
     gene            16086..18050
                     /locus_tag="KR250_001710"
     CDS             16086..18050
                     /locus_tag="KR250_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBH0067646.1"
                     /translation="MLTDMIQNREIKIIKFLLKEGHTTVKAIADDLDVSEKTVSKSLK
                     EVEAVLKKIGLSLIRKPKIGVYIDGDLKKILPYLDNKGSQIPTTREERVIYIYSVLLK
                     SNDYITIQQLSDELYISRGTIERDLAEVEKLLKNEGISIYKKPSKGIKLNISERDKRQ
                     LTAKFIHKFWSRNWYIKQEQESFYQAFEKIQADVNGIFSEDDLSIIIDILRNFSRERK
                     FEFSDYEFQSLVIHLAIAVERIRKGEYIEESLYTGNIENNQMSNTNYLAKQLEEKFSI
                     LLPKSEIGYINLHLVASDQHRDTNELKNTSTNPCETSTNNISNLREIIKNTLYETAYD
                     KDLIDGLTSHVHSSINRLKYGLSIKNPYVNTIKQNFSLSFEQALDLKKVIEFIYDIRI
                     NDDECAYIALHFEAFRERMKELPQNIRVILVCSTGLGSSRLLSARIKKYFPMIKIEKV
                     LSVQELVSSKIDADLVISTIYLELETIPTIVVSPILNESDIRTLERNIQNVSSCTSKK
                     YKSFRNLIFEENILLNIEASNYEDAIKYITDNLIKKGFAKEGIAKSAIERENLSYTSF
                     QSIATPHANPSYIKKSNISVATLKNPITWGNEKVEVIFFISLENDKNLELDEIYEVFY
                     DFIDNKKNINDILSATSTKEVYDLLVEESI"
     gene            18050..18511
                     /locus_tag="KR250_001711"
     CDS             18050..18511
                     /locus_tag="KR250_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBH0067647.1"
                     /translation="MKISDVLKKEQIVFNLDTNTKEETIKKLSSIFVENGIVDNEEEY
                     VKSVLEREEHSTTGIGNGIAIPHGKSNAVKKSSIIFAKTKHKIEWDALDEKPVDSIFL
                     LAISNIDKDSNHLVLLSKLATKLMDDDNVDALKLANSEQEIINIFSEEGEM"
     gene            18514..18834
                     /locus_tag="KR250_001712"
     CDS             18514..18834
                     /locus_tag="KR250_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HBH0067648.1"
                     /translation="MKRKIIAVTACATGVAHTYMAAQALKKASKKMGHLIKVETQGAT
                     GIDNELTTKDAQIAEVVIFAVDTKVRNAERFEGKEILEVPVNAPIKDAEKVINDALKL
                     IDTK"
     gene            18850..19950
                     /locus_tag="KR250_001713"
     CDS             18850..19950
                     /locus_tag="KR250_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HBH0067649.1"
                     /translation="MLKKIVSELKKHVLTGISYMIPLVIAGAMIMAISRVGGSFYNIP
                     DIWDAKYAESASSIVRLLHDLDGFGGTALGLMFPVIAAFIGFSIADKLAIVPGLVGGM
                     IAKDIGAGFLGALAVGLIAGYTCLFIKNHVKLPKSAASIVPIFIVPVFGTLITVVLIN
                     YVIGIPFATLNTGLENWLNGLSGTNQILMAAIIGAMMAVDLGGPVNKAALTTSLALLT
                     SGIYAPNTAAMVGIVIPPLGLGLATILAKQKYNKQLREAGKSSLIMGLIGVSEGAIPF
                     AVESPLKVIPSCVIGTAIASAMAVGLGSVNATPISGFYGWFTVENWPMYVLSIAVGTV
                     IVAGLVIVLRGNPVIEDDEFEDDDFDEAEWES"
     gene            20070..22670
                     /locus_tag="KR250_001714"
     CDS             20070..22670
                     /locus_tag="KR250_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HBH0067650.1"
                     /translation="MIKAHIVNHTHWDREWYFTSGDALVLSEQLFTDVIDELERNPDV
                     SFVLDGQLSILDDYVQLHKEKIEIIKKLIKEDRLHIGPWFTQTDAFFARGESILRNLM
                     IGIFESKKYGKYMKIGYLPDTFGFNAQMPILLKHVGLDNIFFWRGIHLGKQVQSPYFK
                     WKSLNGESYIYAVNMPHGYGTGMLLEPSLKYVNGRLDPAIDFIKSYTDVDEVLIPSGN
                     DQLNIIGDFKNKIKAINDIGKYSYITSSYQEFLKYIKSIESLESYRGEFREPVLARIH
                     KSIGSSRMDIKLACDKLENKLIKRIEPLLVIAKKSKIEISNQLLINTWKKLLEGQAHD
                     SLAGCVTDTVTDDILHRIREANEICDSIENTIVKKISEGLKLSKNDILVFNTEAKRFN
                     GYKEIQVVSDSKNIYFKDNLDATIIEETYVKPRENVLEETPAGNIFIEEPGYYILKVR
                     INVQLPALGYKVVSFELSDKEMVSLDKSNDTFISNDNYKIIYEDGSLNLQLKDGTYIS
                     NFLTLKDSGNAGDTYDFSPLKNDENIKLSFDKVYTEKALGYEKMVVKGTTLLPLTLEY
                     RKNKYLNGKLNVKVSIVLSKENPLMDVKLCVDNTIYNHRLRVHIKTDIKDNKNIASLP
                     FGYITRKNGVLDNWEDIYSEMPIDLEPLESNITLTNQNRSCTVFTRGIKEYQHIEDEI
                     ALTLLATTDELGKPDLLYRPGRASGDTTKKGHIRIKTDKAQMLKELEFSFAIYMDSKS
                     FDELEIANLTHNYLRECVNYQLQDYNFFLYHIDNKIQKSIVNKTVSRELEIISLPENY
                     LISACYPSYYSKDKFIIRVENPSSNRMILDEQIFKNRNGKIVNAIEDVEEEQVYEIAP
                     FDVISILLDL"
     gene            23139..23474
                     /locus_tag="KR250_001715"
     CDS             23139..23474
                     /locus_tag="KR250_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0067651.1"
                     /translation="MYNIDDMIYNCPVEALSNILGKKWVAIIIWKIQEDKKRFGELQR
                     AIPDCSKKMLVQQLDFLIEQGIIVNQKKLVNNVVESTYFLSESGLRLLPVMEKMIMWS
                     NKNLNCDKY"
     gene            23611..24888
                     /locus_tag="KR250_001716"
     CDS             23611..24888
                     /locus_tag="KR250_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HBH0067652.1"
                     /translation="MQNVNLFLVFIEGIVSFFSPCILPILPIYLSILSNSSVENLKEG
                     KTSFIGSSLFKNTIFFALGISTTFFILGSSVKVLSMFFNENKDLIMFIGGIIIIIMGL
                     FYMGIIKSSILNREKRFNVKFKEMKAITAFILGFTFSFGWTPCIGPILASVLVMVSSS
                     SNHLSANLLIAVYTIGFILPFIITAMFYSKLFKTIDKIKSNMEIIKKIGGIILIVSGI
                     LMMVNGFGSISKHFNTSQNSKIESKQEENKRENSTDKEENSDGNDSQKDSNNDNNDKG
                     SNDEDRIKSIDFTLTDQYGKTHKLSDYEGKVVFLNFWATWCPPCKEEMPYIEQLYKDY
                     NKNNDDVVILGVASPNLGREGSREHVVNFLKEQGYTFPVVLDEDGALAYQYGINAFPT
                     TFIIDKEGYVTQYIPGAMDKATMASFIENQRNK"
     gene            25009..26397
                     /locus_tag="KR250_001717"
     CDS             25009..26397
                     /locus_tag="KR250_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBH0067653.1"
                     /translation="MKKTFDAIIIGFGKGGKTLAGDLANRGLKVALIEKSNKMYGGTC
                     VNVACIPTKSLENSANSVKTKNINSWDEVQAEYEKAIDKKETLITKLREANYNKLNSN
                     ENVTIFTGMGTFIDEKTVQVKTENEIYELVADNIFINTGSRPFIPNIKGIENKNIVYD
                     SESLMNLRVLPKKMTIIGAGFIGLEFAGIYSSFGAEVTILNSNNGILPNEDVEDSEEI
                     IKLLAKRNVKIVNNANIKEIKEVSELAIVEYEVDGKSKELTSNMILVATGRKANTEGL
                     GLENAGIELNERGFIKVSETLKTNKEHIWAIGDINGGPQFTYISLDDYRIVINQLFGD
                     KTRTTNDRKNIPNSIFISPAFSRVGLNVKQAKEKGYEVLVAKMPVEAIPRAKQIGKAD
                     GFIKIVIDKKSNKILGASMICENSSEIIHLIQLAVDLEVEYTYLRDRVYAHPTMTEAL
                     NDILSPNMIKEV"
     gene            26601..27314
                     /locus_tag="KR250_001718"
     CDS             26601..27314
                     /locus_tag="KR250_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017208807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF434 domain-containing protein"
                     /protein_id="HBH0067654.1"
                     /translation="MNKISKRGFDDSDKRWFSSKELTRLIKAKEEIEWLLNRDYKIDP
                     VVTFVGDRYQFSIRQRDALKRAVCTDEKNIIRQSKRLSLDKIKEDTVNIDGFNLIISI
                     EVGLSGGTLVIGSDGNIRDLAGLRGTYKIIDKTEEALNLIGKFFNKYNAQNLKFYLDA
                     PVSNSGNLKYRILEHAKTWGIETEVELVKNADVVLEKLDRVVSSDAVIVDKCISYFNV
                     ARGIIEEYIKECNIINLNK"
     gene            27432..28301
                     /locus_tag="KR250_001719"
     CDS             27432..28301
                     /locus_tag="KR250_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HBH0067655.1"
                     /translation="MRGWICVKLTKLNIKKYRAPIYKYALANINLRSAKSTNSSIITV
                     IPQGAKMEVLDEEDDWIKVMYNSQEGYVYKELVSVSEYAWSNLNLREDKSTTSNIITV
                     IPEKSRVEVLQVDGDWSKVVYDDKIGYVFNYFLSIDGNKPNELDYKYFYTDMIKFVNE
                     NNIKSTSDYLIVTDLRNKYTYIFKKDNGGWGQLYKWQCTIGKPETPTITGIFYISGRK
                     PSFGTDEYSVKYATRIKGGYYYHSVLYDSTGSYIIDGRLGEALSHGCIRLSTENAKWI
                     YDNIPDTTTVIIH"
     gene            complement(28456..29601)
                     /locus_tag="KR250_001720"
     CDS             complement(28456..29601)
                     /locus_tag="KR250_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HBH0067656.1"
                     /translation="MSIYYVPPINLLGKGCLNEAKDSIKSLGTKKAFIVSDKFLVSNG
                     TVKKVTDILSQIDIDFVVYDEVKQNPTVTNVNKGLKILKSENCDFVITIGGGSPQDCG
                     KAISILATNGGDIRDYEGINKTSKKCLPIIAITTTAGTSAEVTINYVITDEDRHVKMI
                     MVDTNSLATMTVNDPELMISKPSNLTAATGMDALTHAIEAVVANGAYDVTDSTALYAI
                     KQIFKYLPRAVKNGNDIEAREQMCYACFLNGIAFSNAGLGNVHAMAHQLGGLYDLPHG
                     VCNAMLLPIVEEENAKSAPAKFRPIAEVIGMDVNNKSDKECVDFVIDKIKALSEEVGI
                     PKSLKDVGVDNPNFELLAENSMKDACAGANPVFFDKDKIIELFMKIS"
     gene            complement(29934..30710)
                     /locus_tag="KR250_001721"
     CDS             complement(29934..30710)
                     /locus_tag="KR250_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HBH0067657.1"
                     /translation="MTSDNHRPTARILDILEALAESENGYTLTEIADVINAPKSSIFP
                     IVHTLNARKYITIDKNTSKYSIGIRSYTLGSSFFEKRTIFNYIIDEMTNIVNSCSETC
                     QLGILDNNEVLYIGKVDSPEPIRLISYVGKKIPANCTGLGKALLCDFDKEKLISLYPD
                     GLKGFTEKSITDFDVLYDQLLEVQRTQIASECEESMEHLKCLAVPIRKNGCIRAAVSV
                     TLPLFRANDEKIELIKKLLLNAQKNIQSMMEERNEDIVIN"
     gene            31292..32266
                     /locus_tag="KR250_001722"
     CDS             31292..32266
                     /locus_tag="KR250_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate permease"
                     /protein_id="HBH0067658.1"
                     /translation="MIMDFIKKIPAGMMIVPMFIAAFINTFCPDIVQIGSFTTAVFSS
                     VGSATIIGVQLVCLGTQLQFREMPKVLKRGGILLGSKFAIGALIGILIGKLFGMDGVL
                     GLTTLAVISAVTNSNGSIYLSLMTSYGDETDCATMSLLTLNDGPFFTLIALGTSGLAN
                     VPLLSLLAAIVPILVGMIIGNFDKKMKEFLEPGCNLLVPFVGFALGSSINLASILKGG
                     LSGVLLGLISVFVGGIFIVFCDKFIGKRPGYAGWAVATTAGNAIAVPAAVALVDPSWQ
                     PYVATATTQVAAAVVVTAILVPLITSWWAKKYGCPKFPKQNEDGTITA"
     gene            32364..32669
                     /locus_tag="KR250_001723"
     CDS             32364..32669
                     /locus_tag="KR250_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HBH0067659.1"
                     /translation="MINAVIIDEKDNVAVAIEAIAKGSEISFKLKDKTVKTITALDDI
                     TIYHKLAIKDMAKGDKVTKYGEHIGEANQEIKSGQHVHIHNVRSVREDLDKQVMTNI"
     gene            32825..33985
                     /locus_tag="KR250_001724"
     CDS             32825..33985
                     /locus_tag="KR250_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HBH0067660.1"
                     /translation="MKFYGYKRPDGRVGIRNHILILPTSVCASDTTRIIASQVQGAVT
                     FNNQNGCSQVHSDQQLTMDVMAGYAANPNVYGVIVVSLGCENCQNDLVVDAIKERTNK
                     PIKTLVIQEEHGTLKTIEKAVRYAREMAQEASLLRKEEFPISELILGTECGGSDPTSG
                     LAANPLIGELSDKLVDLGATSILSETTEFIGAEHILARRAVNEEVKEKILHIVHRYEN
                     SLKLVGEEVREGNPSPGNIAGGLTSLEEKSLGCIHKGGHRQISEVYDYAKQIDKKGLV
                     IMDTPGNDASSVAGMVAGGAQIVVFSTGRGTPSGNPIAPVIKITGNKITFANMEDNMD
                     FDASPVIYGPQTMEELTDDLLNMVVDVANGKQSKAESLGYTEMAIARVCNYV"
     gene            34035..34937
                     /locus_tag="KR250_001725"
     CDS             34035..34937
                     /locus_tag="KR250_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HBH0067661.1"
                     /translation="MKFVSFTEGENECKRTGVFSKDELFIIDVNSLNLSRNFKDLNEL
                     IQNVSSEDIIKLKTIINESTFYNYDVFKRERCTVHVPIEKPIHDILCVGVNYKDHLEE
                     TQTHFDDSFEEPQKTVYFTKRVTKAIGPEDEIDGHFEINNQLDYEVELGVVIGKTGVN
                     IRKEEVEDYIFGYTVINDISARKLQKEHVQWFRGKGLDTFTSLGPCILHKSAVPFPIK
                     LKVCSRVNGEERQSSNTGLFISDVSDIVSELSKGMTLEAGDIIATGTPSGVGMGFSPP
                     RYMSSGDVVECEIESIGILKNYIK"
     gene            35017..35910
                     /locus_tag="KR250_001726"
     CDS             35017..35910
                     /locus_tag="KR250_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HBH0067662.1"
                     /translation="MKAEFLTPVVTIFDEEGNLDKEGNKKVYDHLIDGGVDGLVIMGS
                     TGEFFNMSMDMQKELIDLVTSHVNKRVKVFIGTSRMCINESIELSNYAHNSGADGVMI
                     ISPYYFSLDDDSIELFYSEIAKNTEAEIYLYNYPDRTGYDLSPELTLRLARKHKNIVG
                     YKDTVTLMGHTRDLINTMKIEFPEFKVYSGYDENFIHNLMSGGAGCIGGLSNLIPEVC
                     SKWAEAYNNRDMEKVIEIQKYINKAMDFYLITNPFIPAMKKAMNIRGLQISDYMTAPF
                     VKPNEKQVCEIKKLMNELNIC"
     regulatory      35963..36144
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            36372..37502
                     /locus_tag="KR250_001727"
     CDS             36372..37502
                     /locus_tag="KR250_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBH0067663.1"
                     /translation="MKFKSNVLKQFFLIAMVIFMVGGLIGCSKSDKENKQASTGKKLE
                     LKYASGFSVEDLEDGIKKVTDGDNRELILIPKKIKIPEKYKNANIVRTPVDNVLIAST
                     TQACSLRTIDELDSIKAVTTEEQYWTIKEIKDLMKDGKIHYVGSNLAPDYEKIVDLNP
                     DLTIVSSGLSEADTKFIDKLKELGLNYVVDNEYKEQNPFGRMEWTSLIAAFYDKEDMA
                     TSELNKTVQKVEEVSKKIKNKAKPKVVWASVYEGSAYIAPKDSYVDNMIKMAGGINAC
                     ASIDSNEGRVSIEQLHEVAKDADIFVYSSSSDYAPNLDSIKSSAPVLADLDVVKNGNL
                     WVFAPDYYQSVDKTDELIVDLVEMFHPGTTGEKIKHYINYDK"
     gene            37574..38599
                     /locus_tag="KR250_001728"
     CDS             37574..38599
                     /locus_tag="KR250_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBH0067664.1"
                     /translation="MDFINKMRIRNTTIFIVLIISMILTFLVCVAFGSVKIEFKEVID
                     VVLRGMDNTEHYKIIYNIRIPRVLATMVGGACLAVSGILLQIFFKNPIVEPYILGVSS
                     GSTLSVALMILGGYTLGFESVNSMSIFVAAFIGALIVMSIVILFANKVKNITTLLIIG
                     IMTGYVCSGITNVLQSFANSDKLKEFTMWSMGSFSGFTWEKVEILIIVGILGLLMTVF
                     IIKPLNAFLLGEEYAKSIGINIRFFRVLIVFISSILAAIVSAFAGPIGFIGLAVPQIS
                     KMIFKTSDNRVLIPVVIFLGSIVTALCDLISRVILSPVELPISTITSLLGAPIVVMLL
                     VKRKDRA"
     gene            38602..39798
                     /locus_tag="KR250_001729"
     CDS             38602..39798
                     /locus_tag="KR250_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0067665.1"
                     /translation="MLKTNNLSVGYNNKVVISNINVEVKNGEILCLLGSNGAGKTTLL
                     RSLSKLISPIKGEIYLNGVNINCISRKALSKKMALVLTNRLLGDLMTVQDIVNIGRYP
                     YTGFFGSLSKKDLIMVDEALESVDALHLKKRYFDELSDGEKQKVLVARALVQEPEIII
                     LDEPTTHLDIKHRLELINILKKLSKEKSISVILSLHEIDIALKSCDKVALIKNNKVIA
                     YGQPEDVVDEDIINSLYELDDKNFNSLLGSVEISNKSKNEVFIIGGGGKATPIYRAFT
                     KKGIGLYSGIIHENDIDYEIGRTMGIKMFTENPFEPISDESFNLAIRNLNDSKIIIDT
                     GFSVGETNKRNIDIIKEALKLDKKVYSFRNRDESKKYYDSLDSKIEHIDKVSQIINSA
                     DINNLL"
     gene            complement(39926..40651)
                     /locus_tag="KR250_001730"
     CDS             complement(39926..40651)
                     /locus_tag="KR250_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBH0067666.1"
                     /translation="MKTISKQELEKTLEKHYLWLKNNNAGEKADLKELRLENMDLRGY
                     CLSNIDFSWSDLINLNLEKADLENSIFNNSYLSDCSLKNSILKNADLSGASFRFCDLS
                     NSDIRGANLENSNLEHATLNGVISDESTRFFKLYCPETGAFIGYKKCFNFRMVQLLIP
                     SDAKRVSATSNACRCDKAKVLSITSIDGKESFKSARAYADENFIYRVGETIVAEDFNE
                     NRWKESTTGIHFFLTREEAIGYL"
BASE COUNT        15816 a         4324 c         7143 g        13388 t
ORIGIN      
        1 aaatatgaca aattaattga tgacaataaa aaatattttg ttattgtagt attatagggt
       61 aaattgcaaa atatatacga aagtaaaatt ttaaatattg acattaaatt aaatatacta
      121 tactatgtaa gtgtgatgag agaatttttt tagaggggat ttatagtgaa tataaaaaag
      181 atatttaata acaatgtagt agtaagctcc ctagaagatg gcacagaaat aattgtcact
      241 ggtgctggag taggatttaa gaaaaaagta ggcgatttga tagatgaaaa tttgatttct
      301 aagaaatatt ttgttcaaga cgatcaaagg gacaaataca atcaaatact taataagacc
      361 tctattgaat actttaaaat ttcagaagaa attatagaaa aggctaatga agttttaaat
      421 actcaagtga atgattcaat aatattagcc ttaactagtc acatagaatt tgctgtccaa
      481 agagaaaaac aaggtataaa attaccaaac ttaatattga atgagacaaa acaactttat
      541 agggaagaat ttgaatttgg attatgggca attgatgaaa ttgaaaaaag gataggaata
      601 aaattgcctg aagatgaagc aggatatatt gctattcaca taatcaatgg tttggaaaat
      661 tctcaaaaag atgaaggtat taatatacta gagttttcaa aacaagttat tcagataatt
      721 gaagaagtac atggatttaa attggatgta aattcattaa attatactag acttataact
      781 cacttaaagt tttttgttca aaggattctc cgaaaagaaa cttacaatga tagtgatatt
      841 gaagatatgt ataagttagt gaataaaaat tacaataaac ctaaagtgtg tactgaagaa
      901 attgcagcat tggtattaga cagatttgaa tataagatta gtaaagaaga agaattatat
      961 ttaatgattc acataaataa aataacaaac agtggttgtt aggattgtaa ctgataaagc
     1021 aggcaaaacc taagtcattg agcttaacta ttagattaag ttttttgatt taggtttttt
     1081 tattagatac aatcaaaata cagacagtga cttttaggat tgtaactggt taagcaggca
     1141 aaacctaaat cattgaattt aactattagg ttaagttttt tgatttaggt ttttttatta
     1201 gatacaatca aatacagaca gtgactttta ggattgtaac tggttgagca ggcaaaacct
     1261 aaatcattaa acttaattat taaattaagt tttttgattt aggtttttct ataaatttga
     1321 ttatgttttt agataaaaat aggaggggaa aattatgaaa aaaaataagt taggtttatg
     1381 gaacttcttc caaatgttag gaaaaacgtt tatgttccca atagcactac ttagtgtttc
     1441 tgggatgatg ctaggacttg gggcaggttt tacagaccct aagatgattg aaatggttcc
     1501 atttttaggg aatgaatatg ttaacttagt tttaaacttc atgttaacaa taggattatt
     1561 tgcatttaac aacttgggag cattatttgc aatggcaatt ccactaggat tattaaaaaa
     1621 agaaaaagag tttggtgctt tttcaggatt agtaggattt atagcaatgc atataggtac
     1681 aaatttttat ttaacaagag ctgatttgtt agtaccagca gaacagatgt caactaatgg
     1741 tcaagctatg attatgggta tacaaacata taatacaagt gttttaggtg gtattgtagc
     1801 aggattaatt gtttatgcaa tatatgataa agttagtaat ttaaagatac cagaatcatt
     1861 aggattctac agtggaccaa gattagttcc aattgttagt ttaatagtta tgagtatagt
     1921 tggattatta atacctattg tttggccacc atttttcaat gcattccaaa gtttaggaag
     1981 atggatttct tcagcaggac cattaggata tttttcatat gcagtggcag aaagagttac
     2041 aattccattt ggactaaatc atcttgtaac atctgtattc cgttttacac caataggtgg
     2101 aactgctgtt attgatggag aaacgtactt tggtacattg aacatgttta tggcatatgt
     2161 agaaaataat cagattatac ctcttgattt agctggtaaa atggaacaag gtaaattgat
     2221 gattcaatat ggattagctg gtgctgcatt ggctatctac cgtacagcaa aaccagaaaa
     2281 tagaaaggca cttaaaggac ttttaatatc aggagtgttg acagttatta taggtggtat
     2341 aagtgaacca atagagtttc tattcttatt catatcacca gcattatttg ctttccatac
     2401 atttatgaat ggtatagcaa acatggtact tccatactta ggcgtgttga tgggattcac
     2461 aggagattta attgcattta taagttttgg tgtgttaaga ggaacagcta caggatggcc
     2521 aatagcagta ttagtagcag ttttatattt tgcaatatac tattttgtat ttaagtgggc
     2581 aattattaag tttaatataa agaccccagg acgtgaggat aaagaaataa aagtatctga
     2641 aggaaatggt tcagatttca aaaatttgtc tacatataaa ggacaacaaa tgatatctgc
     2701 tttaggtggt caatctaata tattatcact agataattgt gtaactagat tgcgtttaaa
     2761 actacaagat gtatcattaa taaatgagga tgcaattaaa gaggctggtg gtatagcagt
     2821 tgttaaatta gatgagcata caatacaagt aattatagga actcaagtgt attctttaag
     2881 aaagcagatg gataaagcta tggaaggtca tttggaggca tgtaatgatt aattttgata
     2941 aaaaagtaga ccgattgggt acatattgta cccagtggga ctatgtagaa gatagatttg
     3001 gtaagaagga tttgcttcct tttacaattt cagatatgga tttagagttt ccatttgaaa
     3061 taacagaggt gttgcaggaa aggttaaatc atagagtttt gggatacagt agatggaaac
     3121 atgatgattt tagaaattca gttacgaatt ggtatattaa aagatttaat tgtagcgttg
     3181 atagtaactg gatatattat agtccaagtg taatgtattc aattagcaaa cttattgaga
     3241 tgttaagtaa tgaaggagat ggaatcctta taaatactcc tgcatacaat gctttttatg
     3301 atgtgataat aaataacaat cgtaatatta taaagtcaca tcttgtaaat aggaatggat
     3361 tctattcaat tgattttaat gattttgaga aaaagtgtaa aaaatctaag atatttattt
     3421 tatgtaatcc tcacaatcca actggtagag tatggagtga gttagaactt gttaagatga
     3481 taggaatttg taagaataat gatgtcaaga taatatcaga tgatatacac atggatattg
     3541 tatatgacaa tagaatgaca cctatcttaa atgttgcaaa ggactattta gattctgtat
     3601 tcatttgtac atcagcgtct aaatctttca atataccagc attgactggg tcatatgtga
     3661 ttataccaaa tgaaggtatt agaaataaat ttgaaaatat cactagatac cgtgattttg
     3721 taaattcgcc agcaattcta gcaatattag ctacaatggt ctgttatgat gactgcgaat
     3781 attggttaga tgaactatta aaatacctta aagagaatct taaatatacg attgattata
     3841 taaaagataa tttagaacca ttgaaattgg tcatgccaga aggctgttac tttgcttgga
     3901 tagatttttc cgaacttaaa ataagtggtg agaaattcca aaaattattg atggatattg
     3961 gagaagttgc tattatgtcg gggtctgtat atggtgaaga aggtgagttt tatttaaggt
     4021 taaatgtggc atgttcaaga gaaaaacttg aagatggttt aaataggatt aaaaaaacta
     4081 tggattatat tgcaaaaaga attttatagt aggaagggag atagttatgg gatatttaaa
     4141 aacaatggtt gatgttatga ctactgaaat gaatgccata agatgtttaa tagatgaagc
     4201 aggaatagaa tatgaaagta tagttagtga aattgcaaat tgtgaaggca aagtaatttt
     4261 tatgggtgta ggaaaatctg cacatatagg taagaaattg gcagctacat ttgctagtac
     4321 tggtacacct agcttttttg tacatgcaac agaggcagtg catggagatt taggaatgat
     4381 tgaaagtaag gatattacaa ttttaatatc aaatagtgga aattctatgg aagtggtaaa
     4441 ctgtataaaa tatattaaag caataggttc taagacaatt gcatttactt caaacagaaa
     4501 ttcagtactg gcaaaagagt gtgattatgc tttaatatat cctgcaaagg atgaagcaga
     4561 ccatttaaat ctagcaccaa caacatcatc tacaattact ttggttttag gagattctat
     4621 tgcatgtgca ttatctaaga gtagtaattt tggttcttca gatttttata agtaccatcc
     4681 aggaggaagt ttaggtgaga agttaaaaac agctaacaat ggataaaagc aaaaaggaga
     4741 gtagttttta tgttcaaaat gtttaaaaaa aaatcaataa aatcacccat atcaggaaaa
     4801 gtaatagagt tatctaaaat acaagatggt gtattttctc aaaaaattat gggagagggt
     4861 gtagctatag attctacagg agacattgta tatgctccaa tcaatggaaa gatagtagta
     4921 gtagcagaga ctaaacatgc atttgtaata gaattagaaa atgggatgga acttcttatt
     4981 catgttggtt tagatactgt aaatttaaaa ggcgaaggtt ttgaggtatt ggtgagtatg
     5041 ggagaacaag tgaaggaagg aacaccaatg ttgaaaatta accgtccttt gattgaatca
     5101 aagggtatat cacttattac tccagttaca ataactaatc attcagagta caatatgaat
     5161 acatgtaatg taggcaatag tgttgaaggt gggaaagata ctgtaattga gtttaaataa
     5221 agaactaaaa tacaaggtta atatttataa atacctataa acttatgttt tataagattg
     5281 attgtataga tttgttgtat agatttgttg tataaaaaag aactctttaa ttataaatta
     5341 aggagttctt ttttattttg taaatattgt ttagatttat atagaaaaac acttagtaat
     5401 aaatagaata aataagtatt tatatagcta aaaatctaaa tagttttgtt taaatatatt
     5461 tttatatttt gtaatttcaa attaataaat aattagattt aataaacaaa aaataaaaat
     5521 gttgattata ttaataaact ttaatataat atatttatga aaaatgtaaa ctgtgaaatg
     5581 gaggtagtaa catgaaaatt gaaaatcttt ctattgatga tattaaacga ggctatatat
     5641 ttaatgatga tttaaattgt tatagttgca ttaaatgtac aaaaacatat gaggtaggag
     5701 aaatatataa aataggtaat agattctttg aagcttcaaa agctattgaa cttcatatgg
     5761 aattagaaca tagtgattat ttagagcaat tgcttaatag tgattcaaaa tacaacacat
     5821 tgactgaaaa tcaaaaacaa ttatttcaat tatttattag tgatttacca gataaggaga
     5881 tagcaaaaca ggttggagta tccacatcta ctatacgcca tcaaaaattt attttcagag
     5941 aaaaagcaaa acaggctaag ctctatttag cactatatga atctgtattt gaagataaat
     6001 ctaataaaag tagtactata ataccaatcc atgaacatgc tcgaatggta gatgaaagat
     6061 atgtagtaac tgaagaagag agaaaacaca ttttggatac atcatttgaa agtatgaatc
     6121 ctttaaaatt aaaagtattt tcttcaaaag aaaagaaaaa ggtcgtaatt ttatctaaga
     6181 ttttggaaca atttgaacgt gggaagaaat attcagaaaa ggaagttaat cagattttag
     6241 aatctatata tgatgatttt gcaacaatta gaagatatct aattgagtat ggttttatga
     6301 gtagaaataa agaatgtaca aaatattggt taacataatt aaaataataa attttactaa
     6361 tgtgtaatgt tatttttatc tagggggagg agtcaaatat gaatgatgaa ttaaaatcta
     6421 ttttatacaa taaaggtgta gacattgttc gctttgttga tatatctgaa tttccaataa
     6481 atcaaacaca aggtttttct aaagcaattt tattttgtat agggttatca aaaaaattta
     6541 ttacagatat atacaataac ttacctacag atagtgatga gtttttggaa aaagaggaga
     6601 aagtagaaga attagctgac tggatttcaa aatatattca aaataaagga tatagagcat
     6661 attctcaatc ggagaaaaac aatctagaac atggatattt tgaaaaagcc tatattaatc
     6721 cagaaatgca atcagggatt agtcctttac cacataagac aatagcaaat attagtggca
     6781 ttggatttat gggaaaaaat aatttgtttg taacagagga atatggatgt gcttttagca
     6841 tgtgtacagt acttacagat gctccaattt ctgttgaaag atatcctttg attgattcaa
     6901 aatgtatgga ttgcaatgtt tgtgtagaga actgtcctgc aaaagctata catggaaatg
     6961 aatggacttt acctggaaaa agagaatcta ttattgatgt ttcaaagtgt ttttgtgtat
     7021 tgaggtgtat gatgagttgc ccatggtcat taagatatgc aaatcaaaaa taactttact
     7081 agagaaggta ttaaattgaa tattataaaa tttttaaaat gtaagattat ataagatttc
     7141 tcaaaataaa aatttgtata gaacactcag aaatgagtgt ttttttattg ggattaaatt
     7201 atattgatac aaagcttaac tattttaatc attatataaa atttgtatta aaaaattaag
     7261 aataacttgg taatagtgaa aatataatga gattataatt gatttataat ataactataa
     7321 tgatattgta aaaatgaaaa ttgtcctctc aaaattccca aagagagtaa aagtcacttc
     7381 agttatagtc aaaaaaattt ttctgtgaaa agaatttaga tttgaaaagg aggaattaat
     7441 catggaaatt gaaactaaaa ctccagattg gattaatgta ctagaattaa acagtaaaat
     7501 agatattact gggaaatatg atgtaagtaa tatgatacaa aatattgtct ctgataagtc
     7561 actagaaggt tccattatat attttccaaa aggtaattac ttatttgaga aaggtattaa
     7621 aattagtcaa caaataacac ttcaaggaga ttcttattat ggaggaggta atcaagtatc
     7681 aaatttagat aaaaaacaat caatcatagg aacaaccaat tttataaccc gaggagtttc
     7741 taatatgagt attattacat tgtcaggaac aagtcaatgt atcaaaaata ttaattttta
     7801 ttatgatagt catgatatag agaaaatacc gcctaaaaat gtatctgcta ttacagaata
     7861 tggagaaaca caaggactat ctcattttga acatctgttt atttctggat tttcagggat
     7921 aggaattgaa attccatact attcaactgg taatgatatt actgttagtt catgtggtct
     7981 tggtatgagg cttggagaga agtcaatgct ttctagtagc aagatttacg aatgtaaaaa
     8041 tggaatggag attactactg gtgttagctt aaataatgtg agaatagaag aaataaaaga
     8101 aataggtatt aataataaag gatttggttt taacctgatt atgaatctta tggtcgacca
     8161 atgtggatat tgtggattta tgtttgaaaa aatgtgctat agccagataa ctgccagaat
     8221 tactagatgc ggtcagtatt ataaaagtgt tgattataat gcttatgaga atatggaaga
     8281 taggaaagaa gaagcttatt ccatttttta tggagatatt ttagaaaatt gcaatattgt
     8341 attgatgaat agtaatattg ataccttgga aaatgattca cttgatgaac ataagatata
     8401 tgtaatcaag gcaaatgaaa caaacaatgt aatgctaaca tgttatgctg aagcagataa
     8461 ctttatacaa tgtgcaaagg gcaacttgct gttagaaaat ggtgaaaata cttataaatt
     8521 ttataatggt gagatttgtt ctgtaggagg tgttcaaatt tctgatatag atgaaaaaga
     8581 tttaatcaaa ataatagaga acactttgta tatagatgat aaaggtaagt atttaatggt
     8641 tcctagatta aaaaagaata ctgtaatata ttcagtacta gattctacag aagaaataaa
     8701 taaattacat ggtggaatat gggaacagat tgatgtaaaa gtattagctg gggaatccat
     8761 gtactattat aaaaagataa aagattaaat tatcaaacta ggaggtagtg attaatggaa
     8821 gagtctataa taattaaaga tataagtggc tggattaatg tccttgatta tggaatttgt
     8881 aatgatgaaa caaaagatgt aagttttaag ttgcaagaga ttatggatgc tgtaccagaa
     8941 gattctatta tatattttcc aaaaggaaaa tacttatttg aaagtggtgt taagattaag
     9001 aaacgattaa ctttatgtgg agattcttat attttggcga atgttcctaa aaatactgct
     9061 ggagtaacac aatttacttt taaaggaaag aaaaatccta aaagtgaaaa tatagcaatt
     9121 ataacagcaa aaagtgtaag acactgtatt aagagcattt gcttttattc agatagttgt
     9181 gatagagata tgacatctaa tcatgaacct ccaacagatg gaaaacctag atttcatcat
     9241 aatgtaatta taaattatag aaatatatcg gctattgtat gtgaagataa acagggagaa
     9301 tcaggtcatt ataaaaatct atcttttagt ggtttttctg gaattgcact tgatatgcct
     9361 aataattcta cagcaaatga tattacaatt tttacatccg gacttggaat tagtacagga
     9421 gaaaattcaa ctattgctaa cagtaaggtt tggggctgtg ttgatggaat gataatttct
     9481 actggaactt ttataaatgg tatgagggta gaagaaataa aaaaagttgg aattaaaaat
     9541 attggtagag gtttaaattt tattacaaat atcacaattg accaatgtgg atattgtgga
     9601 gtttggtttg attctatatc caatgttcag atatcaggaa atatcacaag gtgtgggcag
     9661 tactattatg atgtaagcta tgataattat ttaatgttgg ataatagagt taaagaagca
     9721 tattctttat tctatggtaa tttattggaa tcaagtaata tagttttatc aaataccaat
     9781 aaagataatt gggaagattt caaagaagat aagcataagg tgtatcttgt agaagcactt
     9841 gaaaccaaga atgtactatt aacatgcaat atagatgcta ctgactggat tgttaagtca
     9901 gaaaaaggaa atttaacgta caataatatg cgacaaactg ttatatttta cgatggaaaa
     9961 ctaagtagta tcaatggaat tggtatatct gatgaagatt atggggataa agtcaagata
    10021 aaaaaaggaa atatatatat aaatagtaag gataaggata ttaagatgca taaaccagag
    10081 gtaggtgaca ttatgtcaac aattattaaa gataaaagta ttctttctgc tcattatggg
    10141 ggaacatggg agaaaattgg tgaaaaaata gagtttggaa taactgtaca ctattataaa
    10201 ttgatagaga aataagtaaa tacgtagtat ttgaatagat gtattttatt ggagagctag
    10261 gactatagtt ttaatgtaaa tggtttggta atagcatgga ttgaataata taaaaataat
    10321 ataaaaaatt aatttgatta tgtaaaattc tatgaagtag taaaactgtg actttagtca
    10381 taggaggttc aaactaagta aaagggaaag gaatatataa tattatggaa ggaagaactg
    10441 ggattaaaga tggaagatgg attaatgtag tagaaagagg tattaaaaat gatggttcgt
    10501 acaacgtagg tcctgctttg caggatatta ttgatgaaat agaaacagat ttttgtgtct
    10561 tatattttcc aaaaggtaaa tacctattta caagtggtgt taaaattaat aaagaaattg
    10621 tattgcaagg tgattctaat tcatctacag gcactacaca atttataact cgtggagtag
    10681 aaaatatgag tattattaca ttgacaggga aaaaacaatg tattaaaaat ataaattttt
    10741 attctgatag ttgtgagata caggtaaata aagaaccacc gacaaaaggt aatcctaaat
    10801 atcattatga gatgattatt aataagaatg aaaagaatgt tgagatgaat aatgtatcag
    10861 caatcctgta tgataacagt aagaatatga ttaaaggtct aggtcattat gaaaatattt
    10921 atataagtgg tttttcgggt acaggaatta ggataccata ttactcaatt gtaaatgata
    10981 ttacagtttc atcttgtggg ctgggtattg atacaggaat agacacaatt atttctaata
    11041 gtagaatatc taagtgtcaa aatggaatga ggattagaac tggaactagt ataaataatg
    11101 taaggattga aaaaatacag aaagttggtc ttataagtag tcaagttaag gggtatgagg
    11161 ggtatggaag ttataaaatt aataatatta cgattgacca atgtgggtat tgtggctttt
    11221 catttgattc aatgaaagat agttattttt ctggtattat aagaaggtgt ggtcagtatt
    11281 attataatac agattatgac acatatttaa gtattaaaga tagagtagaa gaagcatatt
    11341 ctttatttta tggaaattat tttacagaat gtaatattga tttagttagt gataataagg
    11401 ataactggga tgatggctta gaatataaac ataaagttta tgtatttaaa acccttcaaa
    11461 taccaaaatt aacattgaga tgcaatatag atgcctatga ttatatcgtt aaatcggtac
    11521 atggaggagg taatttaatg ttaaaaaata attttaacaa ttatatattt attgattcat
    11581 ctaatccaga agttattaat ggaattaatt ttaattatca aaagaatgga aacttgttaa
    11641 ggttaaataa tggtaaatta agttataaaa atggaaagga agaaacagct atattcagac
    11701 ctaattatgg tcttattgta gcatcaacag attcagaatg tgaacaaata gagactagct
    11761 atggaggaaa atgggagaag cttggttctg aagtaattgg agaagaaacg gtatattatt
    11821 ataaacatca tgaaccagat aaaacaaacc aaatacaata aaatttgtat tatataatga
    11881 tataaagttt taatatatag tgtttacctt taatgtaata ttggtatatt tgtatagtat
    11941 taaatttgtt aaaaagtaaa attgtagggt gcttgaaaat agcacctttt ttagtttcat
    12001 tataaaatta gttttgaaag tttaggaata atgtatattt atttttaaag aattataacc
    12061 ttattaagga tattttaaat gctagaaatg aatcagtaaa tcatactgga attatcttaa
    12121 atgttttgat taaattaagt atttagagct ataattatat taaaaatata ttagaacata
    12181 attatattac tatggaggtt aaatatgata gtaagtgtga gtagaagaac tgatatacct
    12241 gctttctata gtaagtggtt ttttaacaga cttaaagaag gttttgttta tgtaattaat
    12301 ccaatgaatc caaaacaggt tagtaagata gagttaaatc cacatactgt tgattgtttt
    12361 gtattttgga caaaggatgt aactccaatg ttatatgatt taaataaatt aaaagactat
    12421 aaatattatt ttcactatac tataacatca tatggaaaag aagtagagac atgtatttta
    12481 gataaaagaa aggtaataga ttcatttaag gaattgtcta gaaaaatagg caaagaaaga
    12541 gtaattttaa gatacgaccc gattttctta agtgaaaagt acacagtaga ttatcatatc
    12601 aaagcatttg cgagtttatg taatcagctt gatggattta cagaaaaatg tataataagt
    12661 tttatcgatt tatataagaa aacaaaattt aatactaaag cattacatat aaagccaata
    12721 gaaattacag aaatagaaac attatcaaaa gagatttcta agattgctca taagcatggg
    12781 ataacacttg aaatgtgttc tgaagaatat aatttaagta aatttgggat tggaaaaagt
    12841 aagtgtattg atgatagatt aatatcaaaa attataggat gtgaagtaga ggtaaaaaaa
    12901 gatagtacac aaagagatat ttgtggatgt gttaaaagtg tcgatatagg acaatacaat
    12961 acttgcagac attattgtct gtattgttat gctaacttta attatgcaaa agtagaagaa
    13021 aactgtaggc tttataaaga agataataaa ttactcatag atactttaag agatgatgca
    13081 aaaataagca ttagagatat gaagactatt aaagtagata agaataaaca aattagtata
    13141 tttgattaaa ataaatatac taattacctt aatatattaa gaatatagat acttttgata
    13201 acaaaattaa acttaataga tattttctat ataagaaaga tggtcataat atgtcaattt
    13261 gaccatcttt ttgatataat taaaacacaa ataatttagt aacaaaggag gcagaacttg
    13321 aatttattaa aaaaatatcc tatacctatt gcaagtttga ttttgtcaat ctttacttta
    13381 ggtaacttat tacaatctta tagtgaatca ttacgtaatg taattggatt tataggattt
    13441 attttgtatg caatttatat agtcaaaata atattactta gaaagggtgt taaagagcaa
    13501 ttagagaaca ctttaatatc aagtacattt ccaactataa caatggcaac aatgatattt
    13561 gctacatata taaaaccatt atctttagaa ttaagtatta tgatttggag cataggaatt
    13621 gtgggtcatc ttgtgttaat agtcttattt agtaagaagt tcttagttaa attctcaata
    13681 aaaacggtat ttccatcttg gtatatagta tatgttggga tagttgcagc tagtgttacg
    13741 gcaccagcat ttaatcaact tttgattggc aaaattgctt tttggatagg atttgtatct
    13801 tatatagttc ttataccagt aattttaaag agagtatgga taatcaagga aatgcctgaa
    13861 ccatctttac caagtatagc tatattagct gcaccagggt cactattatt gtctggatat
    13921 attaattctt tttcagataa aaataatgta gtattatact tgttattcat attatcaatt
    13981 gtattttata ttatagtttt gggatattta ccgaaattat taagattgcc tttttatccg
    14041 agttttgctg catttacatt tcctatggca ataagtgcaa tgggtatgaa actaatgaat
    14101 ggttatttaa ctaaatcagg tcattttata agttggattt catatgcttc taagttagaa
    14161 gagataattg ctacagtgat tattttatat gttttagttt tgtttattat atatcttagt
    14221 aaatctgaaa ctacatctaa agctaaataa gaattattaa atatgtgtat ttaataggaa
    14281 tattgatata aatatatgaa gataagataa accatagaaa cttaatttag cttaatttta
    14341 ttggaggaaa acaaatgaaa acaaaagaag aatatttaaa agaagttaga aacatatcag
    14401 aagaatactt tagaaaagga gagttttttt gctctgaagc agttctccag acaataaatg
    14461 atgctttagg ccaaccttta agtccagaga taactaagtt agcatcagga tttccgatag
    14521 gacttggaaa ggcacaatgt ctttgtggag caatatctgg gggagagatg gctttaggta
    14581 tagtatatgg aagagttcat ggtgaagcta tgaatccaaa gatgtttgag catgctaaag
    14641 gtttgcatga ttatattaaa aaagagtatg gtgcaacatg ttgtagagct attacaaaga
    14701 aatgggatgg tgataacttt atgtctcccg aaagaaaaga gcactgtatt aaaataacag
    14761 gtcaagttac agagtgggtt gcagataagc ttatagaaga tgaaaaactt aatatcaaat
    14821 agtgtaatat tagttggtga atttataaag ttaaatttac ttatagtata agatacttga
    14881 tattctcgta ttgatagaaa aatttatagc atatgtattt aatgaaatat acttatattt
    14941 agagtgtggt ttaatttcat aaatacatat gctattattt taagtatata agcaaattta
    15001 tttaaataaa tagaaacagt taatttatat gtacaaaact atatatgatt agtgtaaata
    15061 aatacctatt ttataatact caacataaag acaccagaca tgattagtat tataccaata
    15121 aaaatagcaa gccattcttt tttgttcttc ttttctttaa aaaatataat ccctcctaaa
    15181 gtggctacta tcacacaact ctgagagata gaaaaactta ttgtaactcc caatgtatta
    15241 gtcgttatga acatacctaa atttgctatt gaccacaata taccagtaat taaattgaat
    15301 aaaacatttt ccctatatac aatctcttta gaaaaatact tgacaataat agatgaagtt
    15361 atcaacatac caacagattg aggtaatata attctatatc cttcaattaa aaatatttga
    15421 ttagttacaa catataatga taaaaataga gatgataata atacaactcc aacacatttt
    15481 aacatatttt tatctgtaga ttttttagtt tgataatttg tgagaagtat accaagtatt
    15541 atacataata ttgataaaga accaatcagt aacattttag aactggtcca ttcattaaac
    15601 aagattacag ctaataatgt cgtaaaagtt aattgtgaac catttgaaat tggcattact
    15661 ttagatacac tagcaatttg aattgcttta aactgcatta attgtcccaa actccagaat
    15721 atgccagaaa tcatacttat acaaaatgaa aaaaatgtaa tttcaggatg tataaccaga
    15781 gttattatag tggaaaatat aaacgcactt atggatgtac ctaaaagctg ttcatgagga
    15841 gaacttttct ttagatttgc aactatgggc atcaatcccc atcccattac aggaagtaaa
    15901 gcaataatta ttgtcagcat taaactattt cctttcatat taaatttaga cagcttttaa
    15961 tttatcatct aaaattaaag tgttcaatac ataaaaatta ccgtatttaa tcaggaaata
    16021 ttagtagttt tttagaaaaa cattttccat taaaataaag acaaagggaa ggagtaaaat
    16081 tgaaaatgtt aactgatatg attcaaaatc gtgaaattaa aataataaag tttttattaa
    16141 aagaaggtca tacaactgta aaagcaatag cagatgattt agatgtttca gaaaaaacag
    16201 tttcaaaatc tcttaaagaa gtagaagcag tgcttaaaaa gataggtctt tctttaataa
    16261 gaaaacctaa gataggtgtt tatattgatg gagatttaaa aaaaatactt ccttacctag
    16321 ataataaagg aagtcaaatt cctacaacca gagaggagag agttatctat atatattcag
    16381 tacttttaaa gagtaatgat tatattacta tacaacaact ttctgatgaa ctatatataa
    16441 gtcgtggaac tattgaaagg gatttagcag aagttgaaaa actgctaaaa aacgaaggga
    16501 tttccatata taagaaacct agtaagggaa taaagttaaa tataagtgaa agagataaaa
    16561 gacaacttac tgctaagttt atacataaat tttggagtag aaactggtat attaagcaag
    16621 agcaagaaag cttttatcaa gcatttgaaa agatacaagc agatgtaaat ggtatttttt
    16681 cggaagatga tttatcaata attattgata ttcttaggaa tttcagcaga gaaagaaaat
    16741 ttgagttctc tgattatgaa tttcagtcat tagttattca tttggcaata gctgtagaga
    16801 gaattagaaa aggcgaatat atagaagaaa gtttgtatac tggtaatata gaaaataatc
    16861 aaatgtcaaa taccaattat ttagcaaaac aattagaaga aaaattttca atattattac
    16921 caaaatctga aattggatat ataaatttac atttagtagc gtctgaccaa catagagata
    16981 ctaatgaact aaaaaatact tcaactaatc cttgtgagac aagtacaaat aatatatcaa
    17041 atcttcggga aattataaaa aatacattgt atgagacagc ttatgacaag gatttaattg
    17101 atggattgac ttcacatgta cattcatcaa taaatagact gaaatatgga cttagtatta
    17161 aaaatccata tgtaaataca atcaaacaaa acttttcttt atcatttgag caagctttag
    17221 atttaaagaa agtaattgaa tttatatatg atattaggat aaatgatgat gaatgtgctt
    17281 atatagcatt acattttgaa gcttttagag aaagaatgaa agaacttcca caaaatatta
    17341 gagtaattct agtatgcagt acaggattag gttcatctag gttattgtct gcaagaataa
    17401 aaaaatactt tccaatgata aagattgaaa aagtactttc cgtacaagaa ttagtatcca
    17461 gtaaaattga tgcagattta gtaataagta ctatttattt agagttagaa acaattccaa
    17521 ccatagtagt tagtcctatt ttaaatgaaa gtgatataag aaccttggaa agaaatatac
    17581 aaaatgtttc aagttgtact tctaagaagt ataaatcttt cagaaactta atctttgaag
    17641 aaaatatatt gttgaatatt gaagcttcaa actatgaaga tgcaattaag tatataacag
    17701 ataacctaat aaaaaaaggt tttgcaaaag aaggtatagc taaaagtgca attgagagag
    17761 aaaatttatc ttacacttct ttccaatcaa tagctacacc tcatgctaat cctagttata
    17821 ttaaaaaatc taatatatct gttgcaacgt taaaaaatcc tattacatgg ggaaatgaaa
    17881 aggtggaagt tattttcttt atttcattag aaaatgacaa aaacctagag cttgatgaaa
    17941 tatatgaagt tttttatgac tttattgaca ataaaaaaaa tataaatgac atattaagtg
    18001 caacttccac aaaggaagtg tacgatttgc tagtggagga gagtatatag tgaaaatatc
    18061 agatgtattg aaaaaagagc aaattgtttt taatcttgat acaaatacaa aggaagaaac
    18121 aataaaaaaa ttaagtagta tatttgttga aaatgggatt gtggacaatg aagaagaata
    18181 tgttaagtct gttttggaaa gagaagaaca ttcaactaca ggtattggta atggtattgc
    18241 tattcctcat gggaaaagta atgctgtcaa aaaatcttct ataatatttg caaagactaa
    18301 gcataagatt gaatgggatg ctttagatga aaaaccagta gatagtattt tcttattggc
    18361 tataagcaat attgataagg atagcaatca tcttgtacta ttatctaaat tagcaactaa
    18421 acttatggat gatgacaatg tagatgcact aaaattagcc aattcagaac aagaaataat
    18481 caatatattt agtgaagaag gagagatgta gaaatgaaaa gaaaaattat agcagttaca
    18541 gcatgtgcaa caggagttgc acatacatat atggcagctc aagcattaaa gaaagcttca
    18601 aaaaagatgg gtcaccttat aaaagttgaa acacaaggag ctacaggaat cgataatgag
    18661 ttaactacaa aggatgcaca aattgcagaa gtagtaatat ttgcagtaga tactaaagtc
    18721 agaaatgctg aacgttttga gggaaaagaa atactagaag taccagttaa tgcacctata
    18781 aaggatgcag agaaagtaat aaatgatgca ttaaagttaa ttgacactaa ataaatggag
    18841 ggaaatgtaa tgttgaaaaa gatagtctct gagttaaaaa agcatgtctt aactggaatc
    18901 tcatatatga ttccattagt aattgcaggt gctatgataa tggcgatatc tcgtgtaggt
    18961 ggttctttct ataatatacc agatatatgg gatgctaaat atgcagaaag tgctagtagt
    19021 attgttagat tattacatga tttagatggg tttggaggta ctgcattagg attaatgttt
    19081 cctgtaattg ctgcatttat aggattttct atagctgata aattagctat tgtaccaggt
    19141 cttgttggag gtatgattgc taaagatata ggtgcaggat ttttaggagc tttggcagtt
    19201 ggtcttatag caggatatac atgtttgttt attaaaaatc atgttaaatt accaaaatca
    19261 gcagcttcaa tagtaccaat atttatagta ccagtatttg ggacattaat cactgttgta
    19321 ttaatcaatt atgtgatagg tatacctttt gccacattaa atactggatt agaaaattgg
    19381 ttaaatggat tatctggtac aaatcagata ctaatggcag ctattatagg ggctatgatg
    19441 gcagttgact taggtggacc agttaataaa gcagctttaa caacatcact tgcattatta
    19501 acaagtggaa tttatgcgcc taacactgct gcaatggtag gaatagttat accaccacta
    19561 ggattgggac ttgctactat actagcaaaa caaaaatata ataaacagtt acgtgaagct
    19621 ggaaaatcat ctcttataat gggtttaatt ggagtaagtg aaggagcaat tccttttgcg
    19681 gtagaatctc cattaaaagt aataccttca tgtgtaattg gaacagcaat tgcttcagct
    19741 atggcagttg gtttaggttc agtaaatgca actccaatta gtggatttta tggatggttt
    19801 acagtagaga attggccaat gtatgtgtta tcaatagcag ttggaacagt aattgtagct
    19861 ggattagtta tagtattgcg tggaaatcca gtgattgagg atgatgaatt tgaagatgat
    19921 gattttgatg aagcagagtg ggaatcttaa aatttaataa ctatttaaga cgtgttataa
    19981 gctatattca atatgaatct tatactaaat aaagtgttaa atattcagtt tagacttaaa
    20041 tataaaagat aaagaacaga ggagaataaa tgataaaagc acatattgtt aatcatacac
    20101 actgggatag agaatggtat tttacttctg gagatgcatt agtacttagt gaacaactat
    20161 ttacagatgt aattgatgaa ttagaacgta atccagatgt aagttttgta cttgatggac
    20221 aactttctat tttggatgat tatgtacaat tacacaaaga aaaaatagaa atcattaaaa
    20281 agttaataaa agaagataga ctacatattg gaccatggtt tactcaaact gatgcattct
    20341 ttgctagagg agagtctatt ttaagaaact taatgattgg aatttttgaa agtaaaaagt
    20401 atggtaagta tatgaaaatt gggtatttgc cagatacatt tggatttaat gcacagatgc
    20461 ctatactatt aaagcatgta ggtcttgata atatattctt ttggagaggt atacatcttg
    20521 gtaaacaggt tcaatcacca tattttaagt ggaaaagctt aaatggagaa agttatatat
    20581 atgcagtaaa tatgccacat ggatatggaa ctggtatgct tttagagcct tcattaaagt
    20641 atgttaatgg acgcttagac ccagctattg attttattaa atcatatact gatgtagatg
    20701 aggtgttaat accttctggg aatgaccagt taaatataat aggtgatttc aaaaataaaa
    20761 ttaaagcgat taatgatatc ggaaagtatt catatataac gagttcatat caagagtttt
    20821 taaaatatat aaagtccata gaatcactag aaagctatag aggtgaattt agagaacctg
    20881 tattagcaag aatacataaa tcaattggtt ctagtagaat ggatataaaa ttagcatgtg
    20941 ataaactaga aaacaagctg attaaaagaa tagagccatt attagtaatt gctaaaaaga
    21001 gcaaaattga aataagcaat cagttactta taaatacatg gaaaaaactt ttggaaggac
    21061 aagcacacga tagtttagct ggatgtgtaa ctgatactgt aacagatgat attcttcacc
    21121 gtataagaga agcaaatgaa atatgtgaca gtattgaaaa tactattgta aaaaagattt
    21181 cagaagggct taaattatct aaaaatgata ttttagtatt taatacagaa gctaagcgtt
    21241 ttaatggata taaagagata caagttgtaa gtgatagtaa aaatatttat tttaaagata
    21301 atttagatgc tactataatt gaagaaactt atgtaaaacc gagagaaaat gtattagaag
    21361 aaacacctgc tggtaatata tttattgaag aaccaggata ttatatatta aaagtaagaa
    21421 taaatgtaca gcttcctgca cttggttata aagttgttag ttttgaatta agtgataaag
    21481 aaatggtgtc acttgataaa tctaatgaca cttttatatc taatgataat tataagataa
    21541 tatatgaaga tggaagctta aatttacaac ttaaagatgg aacttacata tcaaactttt
    21601 taacattaaa agatagtggt aatgcaggtg atacctatga cttttctcca ctaaaaaatg
    21661 atgagaatat aaaactttcg tttgataaag tgtatacaga aaaagcttta ggatatgaaa
    21721 aaatggtggt taaaggtact actttactac cacttacatt agaatataga aaaaacaaat
    21781 atcttaatgg taaattaaat gtaaaagtaa gtattgtctt atctaaagaa aacccattga
    21841 tggatgtaaa actttgtgtt gataacacga tatataatca tagactacgt gttcatataa
    21901 aaactgatat aaaagataat aaaaacatag catcattgcc atttggatat ataacaagaa
    21961 aaaatggagt tttagacaac tgggaagata tttacagtga aatgcctata gatttagaac
    22021 ctttggaaag taatattacg cttacaaacc aaaatagaag ttgtactgta tttactagag
    22081 gaataaaaga gtatcaacat attgaagatg aaattgcatt gaccttactt gctactacag
    22141 atgaactagg aaagcctgat ttattatata gaccaggacg tgcttctggt gatacaacta
    22201 aaaaaggtca tataagaatt aaaacagata aagctcaaat gcttaaggaa ttagaatttt
    22261 catttgcaat atatatggat tctaaatcat ttgatgaatt ggaaattgcg aatttaaccc
    22321 acaattatct aagggaatgt gtaaattatc aattacagga ttacaatttc tttttatatc
    22381 atatagataa taagattcaa aaatcaattg tcaataaaac tgtaagtaga gaattagaaa
    22441 taatatcttt accagaaaac tatcttatat cagcatgtta tccttcttat tacagtaaag
    22501 ataaatttat aataagagtg gaaaatccaa gtagtaatag aatgatattg gatgagcaaa
    22561 tatttaaaaa tagaaatggg aaaattgtaa atgcaataga agatgtagaa gaggaacagg
    22621 tatatgaaat agcacctttt gacgttattt caatattatt ggatttataa gtgtaaaagt
    22681 aagtttattg tttaaagtct gacagatatt aatctgccag actttttaat ttgtataaat
    22741 ttgggtaaat attaatgaat ggttttagta tttaagtatg aatttagtat ttaatataga
    22801 atttattgaa gtagaataat tagtttaaca ggataagcag tttaaataaa gaattattat
    22861 attagagtga ttttatatat aatttaaaat aataaaattt taataaaatg agttaaagat
    22921 tgtatattaa tagagttgat gtagttacaa aaaagttacc aattgttatg tatttacaaa
    22981 actgatatat taattacata aaatctaaaa tagtttgtaa taggagtata tataataaaa
    23041 attttaatta aataaagcta ggtatattta taatattaat aaatatattg ctataaaagt
    23101 ttgtttaata aattaaataa agaaaagagg agaataaaat gtacaacata gatgatatga
    23161 tatataattg tccagtagaa gctttatcaa atattttagg taaaaaatgg gtggctataa
    23221 ttatatggaa aatacaagaa gataaaaaac gatttggaga gctacaaaga gctataccag
    23281 actgtagtaa gaaaatgtta gttcaacaac tagatttttt aattgaacag gggattatag
    23341 tcaaccaaaa aaagttggtt aataatgttg tagagtcaac atatttttta agtgaatcag
    23401 gattacgatt gcttcctgtc atggaaaaaa tgattatgtg gagcaataaa aatttaaatt
    23461 gtgataaata ctaaaattta ctctgtaagt aatgaaaatt taattaagct atgagtagaa
    23521 aaatgtatta aaattacata attttttagt tagattttaa tttagaaatg ggattgtaat
    23581 attggaaata aaataaggag gaattttaat ttgcaaaatg taaatttatt tttagtattt
    23641 atagaaggta tagtatcatt cttttcacca tgtatactgc caattttgcc tatttatttg
    23701 agtatattgt caaatagtag tgtagaaaat ttaaaagaag gaaagacgag ttttatagga
    23761 agttctttat ttaaaaatac tatatttttt gctcttggaa tctcaacaac attctttata
    23821 ttgggttcat cagtaaaagt attgagtatg ttttttaatg aaaataaaga tttaataatg
    23881 tttattggtg ggattataat tattatcatg ggattatttt acatgggaat aattaaatct
    23941 tcaatattga atagagaaaa aagatttaat gttaagttta aagaaatgaa agcaataaca
    24001 gcttttatat taggttttac gtttagtttt ggatggacac cttgtattgg tccaatattg
    24061 gcatctgtat tagtcatggt ttcaagttca agtaatcatc ttagtgccaa tcttttaata
    24121 gcagtatata ctattggttt tattttgcca ttcataatta cagctatgtt ttacagtaaa
    24181 ctatttaaaa ctatagataa gataaaatct aacatggaaa ttattaaaaa aataggtggg
    24241 attatactta tagtatcagg aatattaatg atggtaaatg gatttggaag tataagcaaa
    24301 cactttaata cgtcacaaaa tagtaaaatt gaaagcaagc aagaagaaaa taagcgagaa
    24361 aattctactg ataaagaaga aaattctgat ggtaatgata gtcaaaaaga ctctaataat
    24421 gacaataatg ataaagggtc taatgatgaa gatagaataa agtctattga ttttacttta
    24481 acagaccagt atggtaaaac acataaatta agtgattatg aaggcaaagt tgtattctta
    24541 aatttctggg ctacatggtg tcctccatgt aaagaagaaa tgccttatat agaacaactg
    24601 tataaagact ataataaaaa taatgatgat gtagtaattt taggagtagc ttcacctaat
    24661 ttaggtagag aaggttcaag ggaacatgta gttaacttct taaaagaaca aggttataca
    24721 ttccctgttg tacttgatga agatggagca ttagcatatc aatatggaat taatgctttt
    24781 ccaactacat ttataattga caaggaagga tatgtaactc agtatattcc aggagctatg
    24841 gataaggcaa ctatggcatc atttattgaa aatcagagaa ataaataaaa gtatttaagt
    24901 ataagtttat aaatcaattt aaggttacaa aaaagtgcct aattgtacca cctaaataat
    24961 cttgttatag taaaagtata aacaatacaa gaaagggttg aattttatat gaaaaaaaca
    25021 tttgatgcta taataatagg atttggaaaa ggtggaaaaa cattagcagg ggatttggca
    25081 aacaggggat taaaagtagc tttaatagaa aagtcaaaca agatgtatgg tggaacatgt
    25141 gtaaatgtgg catgtatacc aacaaaaagc ttagaaaatt cagcaaacag cgtaaaaaca
    25201 aagaatataa attcttggga tgaagtacaa gctgaatatg agaaagctat agataaaaaa
    25261 gaaactttaa ttacaaaact cagagaagct aactataata aattaaattc aaatgagaat
    25321 gtaactatat tcacaggaat gggaactttc attgatgaaa aaacagttca agtcaaaact
    25381 gaaaatgaaa tttatgagtt agtagcagat aatatattta ttaatactgg ttcaagacca
    25441 tttataccta atattaaggg aattgaaaat aagaatatag tatatgatag tgagtctctt
    25501 atgaatctaa gagttttacc taaaaaaatg acaattatag gagctggatt tataggttta
    25561 gaatttgctg gtatttatag ttcatttgga gcagaagtta caatattaaa ctctaataat
    25621 ggcattttac caaatgaaga tgttgaggat tcagaagaaa taataaaatt acttgcaaag
    25681 agaaatgtaa aaattgttaa taatgcaaat ataaaagaga ttaaagaagt ttctgaatta
    25741 gcaatagtag aatatgaagt agatggtaaa tctaaagaat taacaagtaa tatgatactt
    25801 gtagctacag gaagaaaagc aaacactgaa gggctagggc tagaaaatgc aggtatagaa
    25861 ttaaatgaaa gaggatttat taaagtctca gaaactctta agactaataa agagcatata
    25921 tgggctatag gagatataaa tggaggtcca caatttactt atatttcatt ggatgattat
    25981 cgcatagtta ttaatcagtt atttggagat aagactagaa caactaatga tagaaagaat
    26041 atcccaaatt ctatatttat aagtccagca ttttcaagag ttggtcttaa tgttaagcaa
    26101 gctaaagaaa agggatatga agtattagtg gcaaagatgc cagtagaagc aattccaaga
    26161 gctaaacaga ttggcaaggc agatgggttt ataaaaatag tgatagataa aaaatccaat
    26221 aagatacttg gagctagtat gatatgtgaa aattcaagtg agattatcca tcttattcaa
    26281 ttagctgtag atttagaagt agagtataca tatttaagag accgtgttta tgcacatcca
    26341 actatgacag aagctcttaa tgatattcta tctcctaata tgataaaaga agtataacat
    26401 aagtttaata tataaatcta aaattatcat tataagtaga ttttagaaag aggctatctc
    26461 aaaaaataga gataagtctc ttttttactg tttaaataat aaaaatatat tttaatggta
    26521 ttaaaaatta tcttaaaagg tgaaataatc tgtatttaat gtagtacagt tatatcttta
    26581 aagcaaggtg gtgaaatgat ataaataaga tatctaaaag agggtttgat gatagtgata
    26641 aaagatggtt ttcaagtaaa gaattaacaa gacttataaa ggctaaagaa gaaatagaat
    26701 ggttattaaa tagggattac aaaatagacc cagtcgttac ttttgttggt gatagatatc
    26761 agttttctat aagacaaaga gatgcattaa aaagggcagt ctgtactgat gaaaaaaata
    26821 ttataagaca atcaaagagg ctttctctag ataaaatcaa agaagatacc gtaaatatag
    26881 atggctttaa tttgataata tccatagagg taggattatc tggtggtact ctagtaatag
    26941 gaagtgatgg caatataaga gatttagcag gtcttagggg aacctataag attatagata
    27001 aaacagaaga agctttgaat ctgataggta aattttttaa taaatacaat gcacagaatc
    27061 ttaaatttta tttagatgct ccagtttcta attcaggaaa tttaaagtat aggatattag
    27121 agcatgccaa aacttgggga attgaaactg aagtagaatt agtaaaaaat gcagatgttg
    27181 tattggaaaa attagataga gtagttagta gtgatgcagt catagttgac aagtgtataa
    27241 gttattttaa tgtggctaga ggtataattg aagagtatat taaagaatgt aatatcataa
    27301 atttaaataa atagataaat ataggtgaga atatataagt atatgtagca atattcttag
    27361 taatacaagt aaagtttgaa aataggagaa cgaatacata tcgaatttca taaaatgttg
    27421 taaaaagaca tatgagagga tggatatgtg tgaaattaac taaactaaat attaaaaaat
    27481 atagagcacc tatatataaa tatgctttag caaatataaa tttgaggtct gctaaatcta
    27541 ccaattcaag tatcataaca gtaatacctc agggagccaa aatggaagta ttggatgagg
    27601 aagatgactg gattaaggtg atgtacaatt ctcaagaagg atatgtgtat aaggagttgg
    27661 tatcagtaag cgaatatgct tggagtaact taaacttaag agaagataaa tctacaactt
    27721 ccaatataat tacagtaatt cctgaaaaat ctagggttga agtccttcaa gtagatggag
    27781 attggagtaa ggttgtatat gatgataaaa taggatatgt ttttaattat tttttatcaa
    27841 ttgatgggaa taaaccaaat gaattagatt ataagtattt ttatactgac atgattaaat
    27901 ttgttaacga aaataatatt aaaagtacta gcgattatct aattgtaaca gaccttagaa
    27961 ataaatatac ttatattttt aagaaagata atggaggatg gggacaactt tacaagtggc
    28021 aatgtactat aggcaagcct gagactccaa ctataacagg tatattttat ataagtggaa
    28081 gaaaaccttc ttttggaacg gatgagtatt cagttaagta tgctactaga attaaaggtg
    28141 gatattacta tcattctgta ctatatgatt caacaggaag ttatataata gatggcagac
    28201 ttggtgaagc tcttagtcat ggatgtataa gattaagtac ggaaaatgca aagtggatat
    28261 atgataatat accagataca acaacagtta taattcatta agaaaaattt tgagtttttt
    28321 attgggataa tgattcttaa attaatagga ttatatatta acttctcata aagagtacta
    28381 tttaaaaagt gtaataagag agtgctatta acaaaacaaa gatattttga ggcactctct
    28441 tataaatata cataattaag atattttcat aaacaattct ataatcttat ctttatcaaa
    28501 gaaaacagga ttagcaccag cacaagcatc tttcatagag ttttctgcaa gcaattcaaa
    28561 attaggatta tcaacaccta catcttttaa agattttggg attccaacct cttcagaaag
    28621 agcttttatc ttatctatta caaagtcaac acactcttta tctgatttat tatttacatc
    28681 cattccaata acttcagcta ttggtcggaa tttggcaggg gctgatttag cattttcctc
    28741 ctcaactatt ggaagtagca tggcattaca tactccatga ggtaaatcat ataaacctcc
    28801 taattgatga gccatagcat gtacatttcc aagacctgca ttactaaagg caattccatt
    28861 taaaaaacaa gcataacaca tttgttctct agcttctatg tcattaccat ttttaacagc
    28921 tcttggaagg tatttaaata tctgctttat agcatataag gcagttgaat ctgtaacatc
    28981 ataagcacca ttagctacta cagcttctat agcatgagtt agtgcatcca taccagttgc
    29041 tgctgttaaa ttagatggtt tgctaatcat taattctggg tcatttactg tcattgtggc
    29101 tagtgaattg gtatcaacca ttatcatctt tacatgtctg tcttcatcag ttataacata
    29161 gtttattgta acttcagcag atgtaccagc agtagtagtt atagctatga taggaagaca
    29221 ttttttagaa gttttgttga taccttcata gtctctaata tctcctccat tagtggctaa
    29281 tatagagata gctttaccac agtcttgtgg agaaccacct cctatagtta taacgaagtc
    29341 gcaattttct gattttaata ttttaagtcc tttattcaca ttagttacag ttggattttg
    29401 tttaacttca tcataaacaa caaagtctat atctatctga gataaaatat ctgttacttt
    29461 ttttacagta ccatttgaaa ctagaaattt atcactaaca ataaatgctt ttttagttcc
    29521 taatgattta attgaatctt tagcttcatt aagacatcct ttcccaagta aatttattgg
    29581 tggtacataa taaatgctca taactaaaat ctccttttat tttcattgat ttttatgtaa
    29641 aaatatatat ctaacaattt tcactaattt actcataata tatcgtatct tagattgaga
    29701 tagggttgct ggttcttata taagaactta attcgtaatt acgattatga ttagaattta
    29761 acatagtatt tttatcatgt caataaaaat ataaaaaata ttataaaaaa aacaaagtta
    29821 ctgtagttaa atcagcaact ttgtttttat agtataaatc aaataaatac attagtttaa
    29881 aacaaaattt ttaattatta tgttttgata tcttccatat acagaatatt aatctaattt
    29941 attacaatgt cttcattacg ttcttccatc attgattgaa tatttttctg agcatttaag
    30001 agtaattttt ttatcagttc tattttttcg tcattagctc taaataaagg cagtgttaca
    30061 ctaacagcag ctcttataca tccatttttt cttattggta cggcaagaca ttttaggtgt
    30121 tccattgatt cctcacactc tgaagctatt tgtgttcttt gaacttcaag aagctggtcg
    30181 tataaaacat caaaatctgt aattgatttt tctgtaaaac cttttagacc atctggatat
    30241 aagcttatta gtttttcctt gtcaaaatca cataaaagag cttttccaag acctgtacaa
    30301 ttagcaggta ttttttttcc aacataagaa ataagtctaa tcggttctgg agaatctact
    30361 tttccaatat aaagaacttc attattgtca agtataccaa gctgacaagt ttcagaacag
    30421 gaattaacta tattagtcat ttcgtcaatt atatagttaa aaatagttct tttttcaaaa
    30481 aatgaagacc ctaatgtgta agaacgtatc cctatagaat atttggatgt gtttttatca
    30541 atagtaatgt attttctagc attaagtgta tgaactatag gaaagatgct actttttgga
    30601 gcattgataa catctgctat ttctgtaaga gtatatccat tttcggattc tgctaatgct
    30661 tctaaaatat ctaatatcct agctgttggg cgatgattat ctgaagtcat aaatttcccc
    30721 ctctttttat taatttaaag tattagaata tgttgatttt agcacactat acgatatttt
    30781 acaatgtaga aaataaatat gtaaatttat atataaataa gctcttgatt tagatacaga
    30841 tagatatttt tttagactgt acattaagga ataactaatt tccaaaatat acctaaagaa
    30901 agtaagtgat atttagttat ataatttaag taaatatatg caatagttat ttttatatgt
    30961 aaatatcaaa atattatgaa cttatatttt atctattttt tacactgtat aaaaacttta
    31021 tagttttgaa tactatataa ttttaaatac gctgtatttt tgcatataag gaagtcattt
    31081 gataatatac aaggtattat cagaaagttt gaaaaaattt tgacaaaaat ttattttttt
    31141 tattgactca taaataggaa aatgctatca ttaattcaaa gattcgtaat taaaaactta
    31201 gttcttgaat acgaaccgaa acggccgtta gaattaaagc taaaaactta ataagagtaa
    31261 ttaaatatca tgttttagga ggattaactt tatgataatg gattttatta aaaaaattcc
    31321 tgcaggtatg atgatagtgc caatgtttat agcagctttc attaatacct tttgcccaga
    31381 tattgtacaa ataggttcgt ttacgacagc agtattttca agtgttgggt ctgcaactat
    31441 aattggggtt caactggtat gcttgggaac tcagcttcaa tttagagaaa tgccaaaagt
    31501 tttaaagaga ggtggtatac ttcttggttc taaatttgct ataggtgctt tgataggtat
    31561 acttatagga aagctatttg gaatggatgg tgtccttggt ctgacaacat tggctgttat
    31621 aagtgcagtt acaaatagta atggtagtat atatctttca ttgatgactt catatggaga
    31681 tgaaacagat tgtgcaacta tgagtttact tactttaaat gatggaccat tttttacatt
    31741 aatagctctt gggacttcag gtcttgcaaa tgtaccattg ttgtcattat tggctgcaat
    31801 agtgccaata cttgttggta tgataatagg aaattttgac aaaaaaatga aagagttttt
    31861 agagccaggg tgtaatttac tcgtaccttt tgtagggttt gcacttggta gtagcataaa
    31921 tcttgcaagt atactaaaag gaggtttatc aggagtatta cttggattaa tatctgtatt
    31981 tgtaggaggt atatttatag tattctgtga taaatttata ggaaaacgtc caggatatgc
    32041 aggatgggct gtagctacaa ctgctggaaa tgcaattgct gtacctgctg ctgttgcatt
    32101 agttgaccca tcatggcaac catatgttgc aacagctaca acacaagtgg cagccgcagt
    32161 agttgtcact gcaatattag tacctcttat aactagttgg tgggctaaga agtatggatg
    32221 tcctaagttc ccaaaacaaa atgaggatgg tacaataaca gcttaattta tattacagtg
    32281 agttgttggt aaaataataa attctttaat tagattttag gatattaata aaattatatt
    32341 atttataaag gagagcaaga ataatgataa atgcagtgat aattgatgaa aaagataatg
    32401 ttgcagtagc tattgaagca atagctaaag gtagtgaaat tagttttaaa ttaaaagaca
    32461 aaacagtaaa aacgattaca gcactagatg atataactat ttatcataaa ttggctataa
    32521 aagatatggc caaaggtgat aaagttacta aatatggtga acatatagga gaagcaaatc
    32581 aggaaataaa aagtggccaa catgtacata ttcataatgt tagaagtgtt agagaggatt
    32641 tggataaaca agttatgact aatatataaa ataatattta tagagttata aaagttgtat
    32701 tcattgcttt tagtgtcaaa tagtttttaa ataggttttt agctgtttga ttagtgagtg
    32761 taataaaaat tataatatat aaaaattata gaaagtatat agactatttt aggaggaaat
    32821 cagtatgaaa ttttatggat ataaaagacc agatggaaga gttggtataa gaaaccacat
    32881 attaatattg cctacgagtg tgtgtgcatc agatacaact agaataatag catcacaagt
    32941 tcaaggggca gttaccttta ataatcagaa tggatgttca caagtacatt cagaccaaca
    33001 attaactatg gatgttatgg ctggttatgc agcaaatcca aatgtatatg gtgtaatagt
    33061 tgtatctttg ggatgcgaaa attgccaaaa tgatttggta gttgatgcaa taaaagagag
    33121 aacaaataag ccaataaaaa ctcttgtaat acaagaagaa catggaactt taaaaactat
    33181 agaaaaagct gttcgttatg ctagagaaat ggcacaagaa gcttctttgc ttagaaaaga
    33241 agaatttcca atatctgaac ttatactagg aactgaatgt ggtgggtctg acccaacaag
    33301 tggattagca gcaaatccat taataggtga attaagtgat aaacttgtcg atttaggagc
    33361 aacatcaatt ttaagtgaaa ctactgaatt tataggtgca gagcatatac tagctaggag
    33421 agcagtaaat gaagaagtaa aagaaaaaat attacatata gtacatagat atgaaaattc
    33481 attaaaattg gttggagaag aagttagaga aggtaatcca tctcctggaa atatagctgg
    33541 tggtttaaca agtttagaag aaaaatcact tggttgtata cataaaggtg gtcatagaca
    33601 aataagtgaa gtatatgatt atgccaaaca aattgataag aaaggtcttg ttataatgga
    33661 tacccctgga aatgatgctt catctgttgc tggtatggta gcaggaggag cacaaatagt
    33721 ggtatttagt acaggaagag gaacaccatc aggaaatcca atagcaccag taataaaaat
    33781 aactggaaat aaaataactt ttgcaaatat ggaagataat atggattttg atgctagtcc
    33841 agttatctat ggtccacaaa caatggaaga attaacagat gatttgttaa atatggttgt
    33901 agatgtagca aatggaaaac aatcaaaagc agagtccctt ggatatactg aaatggcaat
    33961 agctagagta tgcaactatg tataatcttt aagtaagaaa ataaatacat agaattttaa
    34021 taaagggtga gattatgaag tttgtaagtt ttactgaagg agaaaatgaa tgtaaaagaa
    34081 ctggagtttt ttctaaagat gaattattta ttatagatgt taactcttta aatctcagta
    34141 gaaattttaa ggatttaaat gaattaattc aaaatgtatc tagtgaagat attattaagt
    34201 tgaaaactat tataaatgaa agtacattct ataattatga tgtttttaaa agagagagat
    34261 gtacggtaca tgttcctata gaaaaaccaa tacatgatat cttatgtgtg ggagtgaact
    34321 ataaagacca tcttgaagaa actcaaactc actttgatga tagttttgaa gaaccacaaa
    34381 aaacggttta ttttacaaaa cgtgtgacaa aggcaatagg tccagaagat gagatagatg
    34441 gtcattttga gataaataat caactagact atgaagttga acttggagta gtaataggta
    34501 aaacaggtgt taatattaga aaagaagagg tagaagatta tatctttggt tacactgtta
    34561 taaatgatat ttctgctaga aagttgcaaa aagaacatgt tcaatggttt agaggaaaag
    34621 gattagatac atttacctct ttaggtccat gtattttaca taaaagtgca gttccttttc
    34681 ctataaaact taaagtatgt agtagggtaa atggtgaaga aagacaaagt tctaatacag
    34741 gattattcat atcagacgtt tcagacatag tttcagaact ttcaaaaggt atgaccttag
    34801 aggctggaga cattatagct actggaacac cttctggagt agggatggga ttttcacctc
    34861 ctcgatatat gagtagtgga gatgtagttg aatgtgagat tgagagtatt ggaattttaa
    34921 aaaattacat taagtaactg ttttaatata attaatatta aagctaaatc tgatgtatta
    34981 atgaacatat taaaataaat tttaggagtt taaattatga aagcagaatt tttgacacca
    35041 gttgttacaa tatttgatga agaaggaaat ttagataaag aaggtaataa aaaagtgtat
    35101 gaccatctta tagatggagg tgtagatgga ctagtcataa tgggaagtac tggagagttc
    35161 tttaatatgt ctatggatat gcaaaaagaa cttatagatt tagtaactag tcatgtaaat
    35221 aagagagtaa aagtgtttat aggtacaagt cgtatgtgta taaatgaatc tatagaactt
    35281 tcaaactatg cacataatag tggagcagat ggtgttatga taataagccc ttattatttc
    35341 tctcttgatg atgatagtat tgagttattt tatagtgaaa tagctaagaa tacagaggct
    35401 gaaatatatt tgtacaatta tccagataga actggttatg atttaagtcc agaacttaca
    35461 ttaagacttg caagaaaaca taaaaacata gttggatata aggatacagt aactttgatg
    35521 ggacatacca gagatttaat aaatactatg aaaatagagt tcccagaatt taaggtttat
    35581 agtggttatg atgaaaactt tatacataat ctaatgtctg ggggagcagg ttgtataggg
    35641 gggttatcaa atttaattcc agaagtttgc tccaaatggg ctgaagctta caataataga
    35701 gatatggaga aggttataga aattcaaaaa tatattaata aagcaatgga tttttactta
    35761 ataaccaatc catttatacc agcaatgaaa aaagctatga atatcagagg gttacaaata
    35821 agtgattata tgactgctcc atttgtaaaa ccaaatgaaa aacaagtatg tgaaattaaa
    35881 aaattgatga atgaattaaa tatatgttaa atcaaccttg caaagaggtc aacaataaat
    35941 tacaattatc ttaataaaaa ttaaattaaa ataatactag ggtactatat tagtttaata
    36001 gggaaagtga tgaaaattca ctacagcccc cgctactgta tacggatacg aaatcaaaat
    36061 ccactgaaat ttataaaaat tttgggaagg gtgatggagg aaaagccgtg agtcaggaga
    36121 cctgcccagt attatataag ttcaatttag ggtggagatt gtaatttata ataaaatgaa
    36181 gtatcaattc gtttttgtaa agttgatata tgaacttctc cttattaaat taggagaagt
    36241 tttttatttt attaaaaggt tatacttatt aaagatgact ttaggataac tttaataagt
    36301 atactttaaa aagcatatat tattaaagtt ttttagccac tcataaaaaa taagatattg
    36361 gagtgaaaga gatgaaattt aaatcaaatg ttttaaagca gtttttttta attgctatgg
    36421 taatttttat ggtaggaggt cttatagggt gtagtaaaag tgacaaagag aataaacaag
    36481 ctagtactgg taagaaatta gaattaaaat atgctagtgg atttagtgtt gaagatttag
    36541 aagatggaat taaaaaagta actgatggag ataatcgaga actgatttta atcccaaaga
    36601 agattaaaat tcctgaaaaa tataaaaatg ctaatatagt tagaacgcct gtggacaatg
    36661 tattaatagc atctacaaca caggcttgtt ctctgagaac aatagatgaa ctagatagta
    36721 taaaagccgt aacaacagaa gagcagtact ggactattaa agaaattaaa gatttaatga
    36781 aagatggaaa aatacattac gttggttcaa atttagcacc agattatgaa aaaatagtag
    36841 acttaaatcc tgacttaact atagtgagta gtggtttatc agaggctgat acaaaattta
    36901 ttgacaaatt aaaagaatta gggcttaatt atgttgtaga caatgagtat aaagagcaaa
    36961 atccatttgg aagaatggag tggacaagtt taatagctgc attttatgat aaagaagata
    37021 tggctactag tgaactaaat aaaactgtac aaaaggtaga agaagtaagt aaaaaaataa
    37081 aaaacaaagc taaacctaaa gttgtatggg catctgtata tgaaggaagt gcttatatag
    37141 ctcctaaaga ttcatatgtg gataatatga taaaaatggc tggtggaata aatgcatgtg
    37201 cttcaataga ttcaaatgaa gggcgagtaa gtattgaaca attgcatgaa gttgcaaaag
    37261 atgcagatat atttgtatac tcatcaagtt cagattatgc accaaattta gattctataa
    37321 aatctagtgc acctgtattg gctgatttgg atgttgtaaa aaacgggaac ctatgggtgt
    37381 ttgctccaga ttattatcaa tcagtagata agacggacga attaatagta gatttagttg
    37441 agatgttcca cccaggtact acaggtgaaa aaatcaaaca ttatataaat tatgataaat
    37501 agtacagaga ggacacaaaa agataggttt tatagatttt agttaatata gctttgctaa
    37561 gaggagtata caaatggatt ttatcaataa aatgagaata aggaatacaa caatatttat
    37621 agtattaatt ataagtatga tacttacttt tttagtgtgt gtagcatttg gctctgtaaa
    37681 aattgaattt aaagaagtaa tagatgttgt attaagaggt atggataata cagaacatta
    37741 taaaataata tacaatataa gaatccctag agtacttgca accatggtag gtggagcatg
    37801 cctagcagta tcaggtattt tattacagat attttttaaa aacccaatag ttgaaccgta
    37861 tatattagga gtatcttcag gttctacttt atcagtagct ctcatgattt tgggaggata
    37921 cacattggga tttgagagtg taaattcaat gagtatattt gtagcagctt ttataggagc
    37981 tttaattgta atgtctatag tcattttatt tgcaaataag gtaaagaata taacaactct
    38041 tttaataata ggaattatga ccggatatgt atgtagtggt ataacaaatg tgttacaatc
    38101 atttgcaaat agtgataaat taaaagaatt tactatgtgg tctatgggaa gtttttctgg
    38161 atttacatgg gaaaaggtag aaatacttat aatagttggt atattaggtc ttttgatgac
    38221 tgtattcata ataaaacctt taaatgcttt tttactagga gaagagtatg caaaaagtat
    38281 tggcataaat ataagatttt ttagagtttt gatagtattt atatctagta tattagcagc
    38341 aatagtatca gcatttgcag gacctatagg gtttattgga cttgcagtgc cacaaatttc
    38401 aaaaatgata tttaaaacat cagataatag agttttgata ccagtagtaa tatttttagg
    38461 cagtatagtt acagctcttt gtgatttaat atcaagagta attttatcac cagtagaatt
    38521 accaataagt acgataacat ctttattagg agctccaatt gtagtaatgt tattggtgaa
    38581 aaggaaggat agggcttaga catgttgaaa actaataatt taagtgtagg atacaataac
    38641 aaagttgtta taagtaatat aaatgtagaa gtaaaaaatg gagaaatact atgtcttttg
    38701 ggaagtaatg gagctggaaa aactacccta ttaaggtcct tatctaaact tatatcacca
    38761 ataaaaggtg aaatatattt gaatggtgta aacataaact gtatatcaag aaaagcatta
    38821 tcaaaaaaaa tggctttagt acttacaaat agattattag gtgatttaat gactgtacaa
    38881 gatatagtca atataggaag gtatccatat acaggatttt ttgggagtct atctaagaaa
    38941 gatttaataa tggtagatga agcattggag tctgtagatg ccttacattt aaagaaaaga
    39001 tattttgatg aactaagtga tggagaaaaa caaaaggtgt tggttgcaag ggctcttgtt
    39061 caagaaccag aaataattat attagatgag cctacaactc acttggatat aaaacataga
    39121 ttggaactca taaacatact gaaaaaatta agtaaagaaa agtcaatatc agtaatactt
    39181 tcattgcatg aaatagacat agcattaaaa agttgtgata aagttgctct tataaaaaat
    39241 aataaagtca tagcttatgg tcagcctgaa gatgtagtag atgaggatat tataaattcg
    39301 ctttatgaat tggatgataa aaactttaat agtcttttag gttcagtaga aattagtaat
    39361 aaatctaaaa atgaagtttt tataattggt ggtgggggta aagcaacacc aatttataga
    39421 gcttttacta aaaaaggtat aggtctgtat tctggaataa tacatgaaaa tgatattgat
    39481 tatgaaattg gtagaactat ggggataaag atgtttacag aaaatccatt tgagcctata
    39541 agtgatgaga gtttcaattt ggctattaga aatttaaatg attccaagat tataatagat
    39601 acaggattta gtgtaggaga gacaaataag aggaatatag atattataaa agaggcttta
    39661 aaattggata aaaaagttta ttcttttaga aatagagatg agagtaagaa gtattatgac
    39721 agtttggata gtaagataga acatatagat aaagtttctc aaataatcaa tagtgcagat
    39781 ataaataatt tattataatt tatgaaataa aatgtactta tgtgctatag aaaaaagagt
    39841 atagattata atttttttga aattacaatc tatactcttt tgttattttg tttatgtatt
    39901 tatatattta taaaacttta gcattttata gataaccaat agcttcctct ctagttaaaa
    39961 aaaagtgaat ccctgtagtc gattctttcc atctattttc attaaaatct tcagctacaa
    40021 tcgtttctcc tactctatat ataaaatttt catcagcata tgcacgagca cttttaaaag
    40081 attctttacc atctatacta gtaattgaca gtacttttgc cttatcacat ctacaagcgt
    40141 ttgatgtagc agacactctt tttgcgtcgc tgggtattaa taattgtacc attctaaaat
    40201 taaaacattt tttatagcct ataaatgctc ctgtttcagg acaatacaat ttaaaaaaac
    40261 gggtactttc atctgatatt actccattta aagtagcatg ttctaaattt gaattttcta
    40321 aattagctcc tctaatatct gagttagata agtcacaaaa tctaaaactt gcaccactta
    40381 agtctgcatt ttttaatata gaatttttta atgaacaatc cgaaagataa gaattattaa
    40441 aaatagagtt ttctaaatct gctttttcta aatttaaatt gataaggtca gaccacgaaa
    40501 agtcaatatt acttaagcag tatcctctta agtccatatt ttctaatctt aattctttta
    40561 aatctgcctt ttctccagca ttattgtttt taagccataa ataatgtttc tcaagtgttt
    40621 tttctaattc ttgtttagaa atagttttca ttaaaatcac cttcataaat t
//
LOCUS       DAEPIN010000028        40062 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.028, whole genome shotgun sequence.
ACCESSION   DAEPIN010000028 DAEPIN010000000
VERSION     DAEPIN010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 40062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40062
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.028"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            complement(<1..580)
                     /locus_tag="KR250_001731"
     CDS             complement(<1..580)
                     /locus_tag="KR250_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1648 domain-containing protein"
                     /protein_id="HBH0067667.1"
                     /translation="MSKIECLFTIFPVLILMYATFYFMPYFVGKKHIYGVSIDQEHKN
                     YNYFIKLDKEFKKLLSLGFIIDFILVFILVFTFNKLELSYFIGIIGFLLYESILYIHT
                     HKKAKKLKSEIYSKLGHIDIDSKLIVDMDFINKKNKIIKKFKIIYLIPILFTLGMSIF
                     IAFNYNQLPDSIATHWNINGSPDVFIDKSFLNV"
     gene            complement(573..962)
                     /locus_tag="KR250_001732"
     CDS             complement(573..962)
                     /locus_tag="KR250_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBH0067668.1"
                     /translation="MVLNLDFNSDKSIYVQIKEEITKAIASGELKINESLPSVRSMAE
                     NIGVNLHTVNKSYNELKEEGFLNIDRRKGAIVAQLPMKIDNTTRQKNKLDLELLVAKS
                     YLSGISKEEFLSLSSSLFDKYEVKYYE"
     gene            1154..1600
                     /locus_tag="KR250_001733"
     CDS             1154..1600
                     /locus_tag="KR250_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0067669.1"
                     /translation="MLTYLMKRQMHKKDDGMSKPMMITGAVITGVGAYAVYKMVKSHK
                     SNSQMVNTNYYGNSDYDDYKYDEFDYDCDCRDTKQDYEYEELKKKVKEFNSRRINCEN
                     CPHVSQEEMMQYVNSVKDVKEEIDLHEDAKKDNIYKEDEYKKYEEE"
     gene            1864..2589
                     /locus_tag="KR250_001734"
     CDS             1864..2589
                     /locus_tag="KR250_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0067670.1"
                     /translation="MKRKEIVMLKLLIVEDDSTISFGIKYALEQEGFSIDISKDLSSG
                     KEMISSNEYSMILLDVTLPDGTGYELCQYIRGFSQVPIIFLTACDEEVNIVMGLDIGG
                     DDYITKPFRIRELISRIKAVLRRKGNTSEENKKILKFGDLSIYTLEARVYKNDKEIFL
                     TSVEYKLLLILIQNKNTVLSRTQILEKLWDVTYDFVNDNTLTVYIKRLREKIGDDLCE
                     PIYIETVRGIGYKWIGSENSVSI"
     gene            2576..3820
                     /locus_tag="KR250_001735"
     CDS             2576..3820
                     /locus_tag="KR250_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBH0067671.1"
                     /translation="MFLYNPEVKKFLSKYVTLMFIVIIISIGFSIINVSLTKEMVVRN
                     NQAIIGTLSSKYPNLESQIVDIITQGKSMENIDYGQKILIKYNYDKSIRINSEPIISK
                     LVLETIKINIILVCIIFILIFVLVVRYFKSIYNDLSDMTKYVYYSSEGKPFDMKNKNQ
                     EGQIGLLKTELLKMTTILNEKVELLKREKIFLNNTISDISHQLKTPMTSLIMLNDLLY
                     NDVPYEVKIDFLNKIKNQLNRMDWLIKSMLKLSKVEAKVINFKKDKVKFSELIHRAML
                     SMKIPMEIKNQKLTIEGNDNVSYIGDIDWSVEALVNIIKNCVEHTPEFGNIIITYKEN
                     PLFSELIIKDEGEGIDKKDIPHVFKRFYRGRSSSKEDSVGIGLAMSKSIIESQNGDIY
                     VNSEKGQGTEFHIIFHKMYDSD"
     gene            3900..4577
                     /locus_tag="KR250_001736"
     CDS             3900..4577
                     /locus_tag="KR250_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0067672.1"
                     /translation="MEILRVENLTKSYGKNETKVDAIKNVSLSVEKGTFIAITGPSGS
                     GKSTLLHLLGGVDRPTSGKVYINDVDIYNLKTKDLAIFRRRNIGLIYQFYNLIPVLNV
                     KENILLPAELDNRDIDREYFDDLMKTLGLIDREKHLPNQLSGGQQQRTSIGRALINRP
                     SIILADEPTGNLDSKNSKEILELLKLSVKKYNQTLIMITHDKNMALQADRIISIEDGI
                     IKSDEVM"
     gene            4578..7139
                     /locus_tag="KR250_001737"
     CDS             4578..7139
                     /locus_tag="KR250_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0067673.1"
                     /translation="MNLYTLLTIRYLKQNKRRTIVTIIGIILSTALICGIGNIFESFM
                     DYQMRETIKNDGSFHVTFYDVNRKNVEYVTKSAEIEKHAFTKQLGYSKLENSENAILS
                     IKQYDKNAINGYKVSIKEGRFPAKEGEIVLSESIINLIDDKLKIGDKITLKVGDMFDS
                     SKKKIDSMTFHEGDYIANEKDRTFKVVGIIEKPGFERYNGIATAIAYFNPMNNYNDTI
                     NVSVAVRNPKDVYKISNKISKNIYSNKDKEAVSDMIKYNEHLLRLQGASKYSNINNSI
                     KSIIIAVTILVIICTIATVYNSFSISINERKKQFGILNSIGATSSQIKRLVFIEGIII
                     SLIGIPIGLISGTVAIDLLFKIINKYFTESVVTKMSLQIVYNPIITIVSIIIVLLTIF
                     ISILLPAISASNISHLNVIKNTGEYKVGKIKSSRLIKMIFKTEGVLAYKNIRRNRKKF
                     IITLFSLMVSIIIFISFSGFTLLLLRGEEIRNSQRKYDLYLTAKNTARSVEDTISELE
                     DIDGIKNFELATGQYVSIRVSENKINKSKEDLIRKYYQKYKIGDSYEYDFSNNELIFP
                     GDFAVKNKINNLVQGSFNKERAIEENGVILVRKSAFEENGKKGVVELTNYKVGDTVNC
                     EYLDENALSKKVKVKILAITDEERLGLGYQNMGLQFITYDEVAKNLNLKLSKSLIFID
                     SGGSIKTKKEIEALANKNNFNFYDESASNESEKQDLKVIKIFVYGFIVVISLVSVTNI
                     LNTVSTSINLRKRELAVIQSIGVTPKGFRKMIYLESFIYGILSLLFGIPISIGIILIM
                     NKLISGVIEFSPIIPWTAIVICIVSVFIITFIAGYIPMSKLNKENIIDNIRRESI"
     gene            7330..8604
                     /locus_tag="KR250_001738"
     CDS             7330..8604
                     /locus_tag="KR250_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBH0067674.1"
                     /translation="MITISHITAKNMYKSLEERINKFPQGAPPSDTLYKILNVLYTEQ
                     EAKLVAQLPIKPFRVKTAAKIWSVSESEAYRVLDKLASKALILDIEDNKGKKYIMPPP
                     MAGFFEFAMMRTRHDIDQKLLAELYYQYMNVEEDFIKDLFYSTETKLGRVYVQEEVLT
                     NDNEVSILDYERATHIIDESTHIGISMCYCRHRMQHVGKACDAPMDICMTFDNVANSL
                     INNKFARRVDKIECKELLHQAYEHNLVQCGENVRKGVTFICNCCGCCCEAMVAAKRFG
                     NLHPVQTTSFIPNINHENCVKCGKCITACPIDAISKVKEDGKEYIKIDEDRCLGCGVC
                     VRNCHKNSIMLLKRDEKIITPANSVHRAVLMAIEKGQLQNLIFDNNALASHRAMGAIL
                     SAILKLEPAKKILASKQLKSVYLDKLLSMNDK"
     gene            complement(8765..11806)
                     /locus_tag="KR250_001739"
     CDS             complement(8765..11806)
                     /locus_tag="KR250_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp20"
                     /protein_id="HBH0067675.1"
                     /translation="MFKQRLSKLLSSTLVLSMLFTAAPNITFADNTKDNSEKYQSSDI
                     ELHDYSKNAESYTKTKALAKEKIQTLLSKYGAVSAQYALIDNGKIEISGNGGVYSKQD
                     NKNLNKDNMYSIASISKMFTTTAVMKLVDDGKLNLDTPVVKYIPEFKMADDRYKEITP
                     RMLLNHSSGLMGSSFKNTILLADNDSYGHDNFLKELQKQRLKAKPGAFSVYCNDGFTL
                     AEILVERVSGMSFTNFLDKYINNPLNLQNTKTPENSFDSSKLAKAYVPYWEDAVPQDN
                     LNAIGAGGLYSSAENLCTFAQTFMKNSNGILSPASVKAMENKEYLNGLWPEGEDSILG
                     YGLGWDCVNTYPFNQYNLKALTKGGDSLLFHSNLIVLPDENMAVAVLSSGGSSQLNEI
                     IGQEILLSALKEKGKIKEIKPDKTFSKPQQVKMPSSLKENSGLYASSNMIKVDVNDNG
                     TLTVSSPYIENGPEDKYVYIGQDRFVSEKGNSCLKFVKEKNNITYLNMSSYDDVPGLG
                     QTASLYYVAQKVDDNNISNSVKEVWKKRSGKGYYLVDEKYTSQSYMFGSVKASFSLSD
                     ETPGYIVNTKIMDENNSNAFIEIPGVIGRDLSDIKLHKENGTEYLSFGTLTYVSEDSI
                     TNLPAEKSFTCELESNGYAKWYKIGDDIANKKIEVNLPQNSAFAVYDDKGVPVNYSLV
                     TKNNRVRLPKGGVIVFLGSPNARFEVTYQDEVNASALTGTDRYETSIKISQAGWENAE
                     NAVLINDSAIADALAATPFAYKKNAPILLTGSSQINEKTLAELKRLKVKNVYVVGGEA
                     SINEKSLDTIKSNNISVSRISGSDRYQTSMNIAKELNNISNISKISVVNGEKGLADAV
                     SIGAVSAQNDMPIILTNENSNITEINNLFKNKKIDKSYVIGGEYTVSKNIESKLQNPQ
                     RISGSTRNETNAKVIKEFYKDSKIDNLYVAKNGMNKQDDLIDGLSVGVLAGKTKSPVM
                     LVGNSLDYNQKELFKTMRFKSVTQIGGNGNENSFKQIKEIA"
     gene            12013..12312
                     /locus_tag="KR250_001740"
     CDS             12013..12312
                     /locus_tag="KR250_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HBH0067676.1"
                     /translation="MYKNINKKQLKEMMKNEKDVLLLDVRTKDEFKEYKIENAINISL
                     QELINNIDEIYDYKDKKVVVYCRSGHRSVTACNLLAEEGFEHLYNLNSGIIDYMS"
     gene            complement(12550..14778)
                     /locus_tag="KR250_001741"
     CDS             complement(12550..14778)
                     /locus_tag="KR250_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HBH0067677.1"
                     /translation="MRHLKKTNSLKEILNWNNIKNLRPSKGLKNILEIFKSDLKDIRG
                     NYAVLVIVLGLAFLPSLYAWFNIKASWDPYGSTANMLVAVTNEDKGNEIAGQKINVGD
                     QLIEQLKDNKSLGWQFVDEKTALEGVKKGTYYASLKIPKNFTKDLTSLITDDVKKGEI
                     IYTVNEKINAVAPKITDKGASTIQLQVNQTIVKTVSEIVLGISNGVGAGIEHSLPKLT
                     KVENSLIDLQKRFDHINKTVDLASDATSKLSDTVKDIKSDLPTIKKTLNDTKLLSSDL
                     KKFLEDTNDNLDELSPLIKSDLNLMVDLSSSASSLTLNLIDAVNSGSEDVPKLIDNLS
                     EKLSNLQSLNDTLVDFLTKLNQLTSNNRLDDVIDNLEDSSNKIDSSISTLNDIKNKVI
                     SGQQPSISALNNVLSLSNGIGRINLNILNNFDSKISKPINSIFANSIKVANDIITVLD
                     KAEAKLPKVEEILTTSLKLSGNAQESISLIRERLPLAKGMLDDLIDTLSKISNGEDMK
                     KLVSLLESDILTKSNFLKEPVEVKTEKLYPIENYGSAMTPFYSVLSTWVGILLISALL
                     STSVHGNYKPYEIYFGRGLTFLSIALVQGFIIGTGDILLLGVTAKHPILLIVLLMFTS
                     MVFNFIVYSLVSVFGNVGKAIAIVLLVVQIGSSGGTFPIQVTPTFFQRINPIMPFTYA
                     IGATREAVGGIYAPNLTKDILVLLLFMILSICLNVLLKGPINRVIKPFNDKFGDSNLT
                     GH"
     gene            15071..16846
                     /locus_tag="KR250_001742"
     CDS             15071..16846
                     /locus_tag="KR250_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0067678.1"
                     /translation="MRYFKKYIKKYIVLFLTAVFFLTLEAFCDLAQPTIMAKIIDIGV
                     SNKNLDYILSTGAIMIGITFLGAIFAIIRSVISARVSQHFSYDLRQDVFEKINTYSLK
                     QIDKFGRASLITRITNDINQMQQFVNGMMRVFIKSPIMCIGSLIIAIKLNLKMSIIIL
                     IAVLIIFIIILLNMKIGYRLFKNVQESTDKVNSKLRQFLSGIKVVKLFCRYDYENQSF
                     EELNDELFKNSRKATTMMSFFRPTITIIINVSIIIILLIGRHLIYSGEIKIGIIVAYV
                     NYMTRILTSLIMISHVFNVFVRAKASYERISEVLVDENEEVIEREESLDVGEKDLKFV
                     NCDIIFENVDFSYNKNTDEKILKNVSFKIEEGQNIGVIGQTGSGKTSLVNLIQKLYII
                     DKGDIKIGGRSIYSISEESLRARIGVVFQNSSVFGKSILENVVMGNRDICKEDVVKSC
                     EIANANQFITKLENTYDEVLFQRGKNLSGGQRQRITIARAIAKKPQILILDDCFSAMD
                     INTETIIRKNIKEYISNLREKESKKCTVITISQKISSIIDCDKILVLDEGEVVGFATH
                     EDLLVSSPIYKKIFALQNLVSEDVN"
     gene            16859..18682
                     /locus_tag="KR250_001743"
     CDS             16859..18682
                     /locus_tag="KR250_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0067679.1"
                     /translation="MNLSTGNQKVSRLKKKEKPKDFKKTFKRIFMYFKKDKKITRFIF
                     LLVIIDSIISTVIPYTIGKVVDIIDIDTYSKSIFLKGIIILLSFYIIEFSVKLSKGIC
                     TARLSQSIVREMRSNLFLKFKNLPITFFDKSSTGDIMSRVTNDVDNISTGISNSLVQL
                     ITGILNILMTFIMMFVLSPILTFCSVILIPIVMFVSNFIAKRTRVFFKQQQVELGKLN
                     AHIEEMISNINVVKSFNYERKSVNDFKEVNDRLLNISLKSQIYTSLLMPIMNVISNIG
                     FATICIIGGILASKDIITIGVIASFLSYVRQFTRPLNELANLFNTLLSAIAGCERVFE
                     IIDEQEEQEVHNMFKSEINDNKDGNLNSHFVKGEIELKDVSFSYDSKKKILDNINLKI
                     KSGTSNAIIGETGSGKTTIINLITNFYHIDEGSIFLDGKDIYSINRNYLRTCFGVVPQ
                     EGYIFNDTVMENIRYGNLEATDEEVIYASKIANAHKFIVKMREGYSTVLSDRGEDLSE
                     GQKQLISIARAVLKNAPILILDEATSNIDIATEAKIQEAINNLTYGKTSIIIAHRLST
                     ISNCDNIIVLENGRIVESGNHKELIKLKGHYYRNILLTQGK"
     gene            18800..19345
                     /locus_tag="KR250_001744"
     CDS             18800..19345
                     /locus_tag="KR250_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH peroxidase"
                     /protein_id="HBH0067680.1"
                     /translation="MKKFVCTVCGYIHEGDAAPAQCPVCKVGADKFEEMKGEKNWADE
                     HRIGVAQGVDEEIIEGLRANFVGECTEVGMYLAMSRQADREGYPEVGEAYKRIALEEA
                     EHAAKFAELLGEVVVADTKENLRVRVDAEYGATDGKLKLAKKAKELGLDAIHDTVHEM
                     CKDEARHGKAFLGLLNRYFGK"
     gene            19605..20213
                     /locus_tag="KR250_001745"
     CDS             19605..20213
                     /locus_tag="KR250_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HBH0067681.1"
                     /translation="MVDNLIKYIHKSYVAIVVLHEIYGINSFIEDVCQKYYKYGYDVF
                     CPELLGEKRVFPYSKVQEAYDFFINNVGFDIYKKVEKLINDLKSEYKYVFVAGYSVGA
                     TIAWRCSEDSFCDGIICCYGSRIRDYMDVIPKCPTLLVFAKHDSFDVDSISYQLSRKQ
                     NIQIEILDANHGFIDRYSNNYSDIQTKVFTIKEKSFLSKYLA"
     gene            20540..23437
                     /locus_tag="KR250_001746"
     CDS             20540..23437
                     /locus_tag="KR250_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBH0067682.1"
                     /translation="MKAFKKHFLILSLIFLLIISCFNNILCFADESENSKKIRVGYCD
                     DYGIISSSKEHSYTGYGYDYLREISKYTRWEYEFVKGSWEECLDRLEKGEIDLLGPLQ
                     KNDERNKLFNFPKLNSGYEYSVLYTKDSNNNMFYEDISSFKGMRVAVLRGNFHNIAFE
                     KYREENNFSVEYIYCNSIDELIESVNEKKADAFVCGSIINAKGMKIVSKFSVEPFYFA
                     TAKYKPNLVKELDYALKELKINDMYYELELYKKYFKREVNNSIAFTRQEMNFIKANPK
                     LTMVYDSEWSPVEYYDKESNSFKGISSNLMSIISKKCGIKFEYIKTKNYNESLDYIKS
                     GKANMLCGSINENDKAKRFNMKLTNPYINIPMIMVGKLDTNLNNDLNIALTSSYKSID
                     SYIEKSFENAKTTSYKSIESCLNAINEGKANLMILSSYQFDELLREGKSENLSVISVL
                     DTSYGMRIGVSNKTDPILVSILNKSIDKITEEELSDCIYSNTIDKPYKVPFGVIFKEY
                     SIQIISFVCILFLIAIKYIIYNKKKKEDYLKKIAYTDPLTGADSIDKFKINSNKLFAK
                     NNPEEYALFYIDVDKFKYINDMFGYDMGNDTLIHISNTIASELKEDEIFARVSADHFV
                     LLIKYKTDDDIKTRLNNIYNKVQILSNPKINYYKLILDCGIYKISKSDNDINTIMDRA
                     NTARKTIKGGHKNSFAFYDKEMHKKILKEKEIENSMVDALNNGEFIVYFQPKYSLSDY
                     QIIGAEALVRWDNPQKGLIPPIEFIPVFERNGFIVNIDFYVFEEVCKKIREWMDEGQK
                     VVPISVNLSRMHFVNSNFIEKFKLIVDKYKIPTRLIELELTETAVLDNIEGLLDTMNN
                     LKEKGFVISMDDFGTGYSSLNLLKELPVDILKLDRAFFTEKDESNNEKIVISNVIKMA
                     KELKMKVISEGVETISQVEFLKQIGCDMVQGYLFSKPMPVKEFEKIAFKKE"
     gene            23758..23970
                     /locus_tag="KR250_001747"
     CDS             23758..23970
                     /locus_tag="KR250_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBH0067683.1"
                     /translation="MMPLILSNTGEEVVIKKISGSDKTRTFLNSIGFVVGTNVKIVSK
                     IDGNLIIDVKDTRVALDKKMASRIII"
     gene            23983..24204
                     /locus_tag="KR250_001748"
     CDS             23983..24204
                     /locus_tag="KR250_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBH0067684.1"
                     /translation="MNRLKDIKCGETVKVKKLEGEGATRRRIMDMGITRGVNIFIRKV
                     APLGDPIEVTVRDYELSIRKSDAEKIIVE"
     gene            24304..26460
                     /gene="feoB"
                     /locus_tag="KR250_001749"
     CDS             24304..26460
                     /gene="feoB"
                     /locus_tag="KR250_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HBH0067685.1"
                     /translation="MSIKIGLIGNPNCGKTTMFNGLTGSSQYVGNWPGVTVEKKGGKL
                     KGNKDVEIVDLPGIYSLSPYTLEEVVTRNFMLDDKPDAVINIVDASNIERNLYLTTQV
                     LELGIPTVIALNMMDIVNKNGDKINIKELSEVIGCPVVEVTAVKGQGIMEAAEKAVEL
                     ASSNNKLNFKLPFVDESKDAIEKIEKIIEEKTPYIDVETRWLAIKLFERDENVIQKLD
                     ISKTILNSIEEITRNCEDELDDDSESIITANRYEFISSIISSIIKKNRKGKETVSDKI
                     DKIVTNRILALPIFALIMWGVYYIAVSSLGTIATDWTNDVLFGEIIQGNVSNFLASLN
                     VAEWLQGLVVDGLIGGVGAVLGFVPQIMLLFLLLSILEDCGYMSRVAFIMDRIFRKFG
                     LSGKSFIPMLISSGCGVPGVMSTRTIENDRDRKMTIMLTTFIPCGAKIPIIALFAGAL
                     FGGASWVAPSMYFLGIAMIIICGIILKKTSLFAGEPSPFVMELPQYHIPSAKGVLIHM
                     WDRGKAFIIKAGTIIFVACGVIWFLQSFNWSLQMVDAGDSILASLGNIVAPIFAPLGF
                     GNWQSSVATVTGLVAKENVVGTFGVLFGISDATEQDPTLLASVASMFTVASAFAFMAF
                     NMLCAPCFAAIGAIKREMGSWKWTWITLGFQTLTAYIIALLINQVGSLVLGTGGSIAG
                     AIISIFIAVAVVFVVLTYSNKNMKKEKMGKLSYMKN"
     gene            26486..26638
                     /locus_tag="KR250_001750"
     CDS             26486..26638
                     /locus_tag="KR250_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HBH0067686.1"
                     /translation="MATFIIAAIVVVLMALAVMYMVKNSKKNGSSCGCGCSGCSSSKS
                     CHSAKR"
     gene            26818..27528
                     /locus_tag="KR250_001751"
     CDS             26818..27528
                     /locus_tag="KR250_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HBH0067687.1"
                     /translation="MFKFDVTNELMCIARGSGKFFAKKGAMVAFKGNFNFEKLLLGPS
                     NGGGLGRALLGHVQRSLTGEQMPIMVVEGEGEIYLAQNAYHVDVISIDPGDSIYVESE
                     NLLAFSEQLNYSVKLIGSGVISQKGLFTTHLVNKTGQNQDVAIITDGNPLILEGPCCV
                     DPDALVAWTGREPYPKVSKLSWKTFIGQTSGESYHMEFVEPGQIVIIQPSERLSGLKT
                     DSTPSDRMSSGGLRLGID"
     gene            27613..28383
                     /locus_tag="KR250_001752"
     CDS             27613..28383
                     /locus_tag="KR250_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0067688.1"
                     /translation="MFEKIHKRLKGYYIFLIILILWKIVCVLGIWSSYILPPPEIVLD
                     TFIKMIYDGSIFLNVYASVRRILIGFLISSLMAIPLGVLFGVNKKMYEYFKPLFEFMR
                     STPPLALVPMLILWFGIGEESKIIIIILASFFPIFLNTLKGIKNCDNKLIEVGKSFNL
                     SKKRIFYKIIIPNSMLDIVVGLSLGLGYSFRAIIGAELIAASSGLGYLISDGKDMSRT
                     DVVLVGIFVIGFLGILSDYLFSKIITKFSKGKQVDLNG"
     gene            28376..29107
                     /locus_tag="KR250_001753"
     CDS             28376..29107
                     /locus_tag="KR250_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0067689.1"
                     /translation="MVRSGFLLENIYKKYLVDNKEHLVLDNISLNISSEEITVILGES
                     GCGKTTLLRILAGLENATSGNIYFFNNDKKCTPKVGMVFQESRLMPWLNVSENILLHT
                     EKDNRNKVDLDKYLKMMKLEKFKNSYPNELSGGMAHRVSIARALSFNPDILLMDEPFA
                     ALDYFTRRKMQKEVVNIHKNTKKGVVFVTHNIEEAMEIAKKIIVFSKNKRIKQFSVED
                     EYNRDLTKNYYINLKKEILRELGEF"
     gene            29141..30100
                     /locus_tag="KR250_001754"
     CDS             29141..30100
                     /locus_tag="KR250_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrtA/SsuA/CpmA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HBH0067690.1"
                     /translation="MTSLIMVLVGTVGCSTTNDKKDEPAKKEASLPKEINLTYVKSPL
                     NVPSILEKKDDLFGKEFKKDNIKVNFHELTTGPEQTQALAAGELDFLHALGGTSAIIA
                     ASNGVGLKITNIYSRSPKGYMLITNNDNIKSIKDLKGKKIVGPKGTILHQLLIAALAK
                     EGYTIDDVEYVNMDIPSAASALESKSADVAMLAGPVALKTINSGAKMIVNGEGLVSGI
                     IVTAVSDDFAEKYPELVKRFMKVHEETLKYMNENKDEVMDVVSKEVGLSLDETKEMYS
                     WYDFSSKITDKDIKELEDTQEFLMSNGMQQKKINIKDMLYNQN"
     gene            complement(30198..30794)
                     /locus_tag="KR250_001755"
     CDS             complement(30198..30794)
                     /locus_tag="KR250_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3793 family protein"
                     /protein_id="HBH0067691.1"
                     /translation="MSCCNSQFCCKNKIESSYIKWLLEVLGPVFLGSKPAEIINISFT
                     DVNREEKINDIYKYLYKCKKIDFIVIDKEKKGLKILFVNKKALSEKLKCKKTVNFLKF
                     LGYKQNTNVNAYLEHLVDKLKSDVFPDEIGIFLGYPLKDVIGFMGYSNYEVSMIKYWK
                     VYGDTKQSEDTYSKFLLHRKKMRKLLDYISVDKIVSCF"
     gene            31040..31639
                     /locus_tag="KR250_001756"
     CDS             31040..31639
                     /locus_tag="KR250_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0067692.1"
                     /translation="MKLKRTLFVGLALVLAIGITACTKNNEKPSENTGKNPNESVNNN
                     EGYMNYYSTSYNDYIAGLNRYSIYDTPESINNQFKDKEYPGNEKYLSDVKAAYKDSRD
                     KIQSFVTSLKKDGKTEDTELKKMNDDLIAEGERLVKDIDKRIEKLDKVSDEDMKKGQN
                     DFIKLVHGTEDVGNDIGSGFRKMIKDMNDRLGITGNNKK"
     gene            complement(31809..32276)
                     /locus_tag="KR250_001757"
     CDS             complement(31809..32276)
                     /locus_tag="KR250_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0067693.1"
                     /translation="MENNTIKADDIINYCLSNLDDVLLMNSWGERAIYYNPNGILKRG
                     VYVLTIKEKDSNHDKGSLVSRPNVYRVNIGLKKETFIEMFGYIPKRPGVGQIVDMDFD
                     FTKLDTIMPHPIYSWMGWICVLSPTEKTFENFKTLIGESYNFAKQKFKKRKNK"
     gene            32387..33331
                     /locus_tag="KR250_001758"
     CDS             32387..33331
                     /locus_tag="KR250_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family transcriptional regulator"
                     /protein_id="HBH0067694.1"
                     /translation="MSRAERLIELMITINAKRSFTAGELAEEFSVSKRTILRDLQVLE
                     SIGFPLYSKVGAAGGYHVLKERILPPIAFSESEVKSIFFAYQSLEYYNDLPFEQETIS
                     VLKKFLNCIPNDIQYNIENIRRKFVFWTPDRHCSTPLLKELFNIVMNESTIKIEYSSK
                     QKNSVRTIVPIGLYAMNGLWYCPAYCIKSASIKEFRVDRIVKILSIENLSSKKYKVLS
                     SIHDYLKNMEVGTDYHIKINLTDEGVKRCETEFLLARGLKILSKGGYIDMYIPKSTLN
                     WVAEYFLTFGKNATIIEPIELKHLVKSKVLELYNHHCI"
     regulatory      33621..33727
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            33833..34798
                     /locus_tag="KR250_001759"
     CDS             33833..34798
                     /locus_tag="KR250_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HBH0067695.1"
                     /translation="MISIKNVNKYYGKIQVLKDVSIEIESGEIFGIIGHSGAGKSTLL
                     RCINGLEEYQEGSVLVSDKEVKSLNEKQMRDLRKELGMIFQHFSLLERKTVFDNVALP
                     LECFGYSKAEIKKRVLELLEVVGISEKKNDKPRNLSGGQKQRVAIARALALNPQVLLC
                     DEATSALDPNTTKSILSLLEDINKNLGITIIVVTHQMEVIKQICGRVAIMENGEVLEV
                     GDTEEIFLRNTKGLRKLIGEESIILPKGTNIKILFPKDISNEAIITTMARELNIDVSI
                     IFGKLEQFKDDILGSLIINISDKSGEQVKQYLTSKGIRWEEMINE"
     gene            34791..35447
                     /locus_tag="KR250_001760"
     CDS             34791..35447
                     /locus_tag="KR250_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0067696.1"
                     /translation="MNSLIDFLTTLFPNALLQTLYMVIVPTIVATILGFILAIILVVT
                     KPDGLKPNSTINSALGFIVNIFRSFPFMILIVAMIPITRLIVGTSIGETAAIVPITIG
                     AAPFIARIIESSLNEVDKGLIEAAKSFGATKRQIVFKVMIKEAMPSIVSGITLSIISI
                     LGYTAMAGAVGAGGLGNIALIYGYQRFDTAVMVYTVIALIILVQIIQGVGNLAYKKLK
                     "
     gene            35483..36274
                     /locus_tag="KR250_001761"
     CDS             35483..36274
                     /locus_tag="KR250_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HBH0067697.1"
                     /translation="MKLKKLLSVALVSAIAISAVGCSNKEDKKILVGASSNPHAKILE
                     VAKPLLKEKGYDLEVKIFDDYVLPNTALDEGSLDANFFQHIPFLEETVKEKGYKLTYT
                     SKVHIEPMGFYSEKVKSLDELKDGAVIAVPNDATNGARALKLLAKNKLIEVKDGELIT
                     KKDITKNPKNIVIKEMNAEQLPTVLKDVDGAVINSNYALTANLNPTKDAIVIESSDSP
                     YVNIIACRENNKDSDKIKALSEAMNSKEVKKFIQDEYKGSIVPAF"
     gene            36422..39160
                     /locus_tag="KR250_001762"
     CDS             36422..39160
                     /locus_tag="KR250_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HBH0067698.1"
                     /translation="MESKGTLSKNKVYILANGLFLVIALLNINLYTVSINMYRIILLW
                     IFGYSIVYISSFKTDEFINILKVGCLVLVFVSILGVVNLKIQKFQIVLLNLSIINMST
                     FIYLTISKVKKLRISPILIFYSAFVILDYFIRNIFSDINWNMIIILINVCISMFNLLY
                     LLNKLDSNIEHYKLIKDLTYFVFFTTIICFVAISKINIVDSDRLIGVIYLLLCNHTYY
                     VYVYSLNNRVVYPYYELDSINKKLSKKSEQLGKINLAIEKDMIIQRTLKNYIDQRKEL
                     LRQALDTIPNVWIVTDYDFNISYTNNKFKDEFSKDMNNYYKILTFIKEDEKVAEKLKK
                     FDNDISIIDKLVELNDKIYLLSLSNNKDESNYLISLNDITNEIKIDEEIRNINKDYEN
                     IILNIPSPILVRSAEGGIKKNKVISINKKYEKSFKCVSSDLVDMTLEQYFETFNIDFF
                     DNREFKRLNLTQEKKAEIVSYNDTANCIVNFVMSDSEGEEHVEEVRVGDYWSDNKLFK
                     LLTFRDITKEINILRTVNEQKIIYEKLLDVIPEAIFLEDLSTSRVLYTNRAFRELFGI
                     SSDVLGVTTQKYRNILVKKYINNLNIGEREKSIHIVNENNHIKEVKMISRTLYFGQKR
                     SRVRIIKDLSVQRESERLKKALIKQRQYDQMKMEFYANISHELKTPLNNIYSSVQLIE
                     NLYKKGKIIDFQDILKEHIKITKQNMFRLLRLIDNIINISQVKSDIYKIKAVNFDIID
                     ITERIVTSISSYAKSKGIDLIFDTDEETVMVGLDPESIERIILNILSNAIKFTLEGGE
                     ILVGIYKKDETVEIIIKDTGVGIDKEKLNDIFNRFKQIENSGISNEFGSGIGLCLTKS
                     LIEIQNGKIYIDSKVGEGTNVKVIFPIKEVVEEVYDNSNYNDNIEKFEIEFFDIYK"
     gene            39269..39877
                     /locus_tag="KR250_001763"
     CDS             39269..39877
                     /locus_tag="KR250_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HBH0067699.1"
                     /translation="MEKDFFRKNGIDLAKSILGKYLIRKYENKVIVTKIIETEAYMGV
                     NDKGAHVYGNKKTDRTKPLYLDGGHIYVYLIYGMYNCLNLSANIENVPECVLIRGVEP
                     ITSLDEISMNRYNKAYTELSKYQVKNITNGPGKLCKALKIDRSLNSKSIMGEELYISD
                     FYYDDKGKKVFSKDELDIKTSKRINIDYAEEAKDFLWRFYIE"
BASE COUNT        15738 a         4247 c         6544 g        13533 t
ORIGIN      
        1 atacattaag aaatgattta tctataaaaa catcaggaga cccatttatg ttccaatgag
       61 tggcaattga gtctggcaat tgattataat taaatgctat aaaaatactc attcccaaag
      121 taaatagtat cggtattaaa tagattattt taaatttttt tattatttta tttttcttat
      181 ttataaaatc catatctact attaattttg aatctatatc tatatgacct aatttagaat
      241 aaatttctga tttaagcttc ttagcttttt tatgtgtatg aatatatagt atactttcat
      301 ataataaaaa acctataata ccaataaaat aggaaagttc caatttatta aatgtaaaga
      361 ccagaataaa aactaatata aagtctataa taaaacctaa agataatagt tttttaaatt
      421 ctttatctaa ctttataaag taattataat ttttatgctc ttggtctata gaaactccat
      481 agatgtgttt tttacctaca aaatagggca taaaataaaa tgtagcatac atcaaaatta
      541 gaactggaaa tattgtaaat agacattcaa tcttactcat aatatttcac ctcatacttg
      601 tcaaataaac ttgaacttag tgacaaaaat tcttctttac taatacctga taaataagat
      661 tttgccacca ataattccaa gtcaagttta tttttttgac gagttgtatt atctattttc
      721 attggtagtt gagctacaat agcacctttt cttctatcaa tatttaagaa tccctcttct
      781 tttaattcat tgtaggattt gttcactgta tgtaaattta caccaatatt ttcagccata
      841 cttcttacag atggaagtga ttcatttatt ttcaattctc ctgaagctat agccttagtt
      901 atttcttctt taatttgtac ataaatagat ttatcactat taaaatctaa atttaaaacc
      961 ataaaaaacc tccattatgc tttataaatg aaaacgattg ttatatacat agtataacat
     1021 aaaaaataat aacaaacaca aaatataagt ttatattttg taaaaattgt aatttaaaaa
     1081 ataacataat ttttattaaa ataatacaga tactatttat agatagaaat aaaaattaga
     1141 aaggagaaaa aatatgttaa catatttaat gaaaagacaa atgcataaaa aagatgatgg
     1201 aatgagtaaa ccaatgatga ttacaggagc tgtaattaca ggtgtaggag catatgctgt
     1261 atataaaatg gtaaaaagcc ataaatctaa ttctcaaatg gtcaatacaa attattatgg
     1321 aaatagcgat tatgatgatt ataaatatga cgaatttgat tatgactgtg attgtagaga
     1381 cacaaaacaa gattatgaat atgaagaatt aaaaaaaaaa gtaaaagaat ttaattcaag
     1441 aagaataaat tgtgaaaatt gtccacatgt ttctcaagaa gaaatgatgc aatatgtaaa
     1501 tagtgttaaa gatgtaaagg aagagattga tttacatgaa gatgctaaaa aagataatat
     1561 ttacaaagaa gatgaataca aaaaatatga agaagaataa gtaatatttt gtataactga
     1621 acaaatagta atgtcaaatt atttaatatc tagttttaat atgtaagttg taacttaact
     1681 tgaaagaatc agtatcattc tttagatagt taagtaacaa cttacatttt atttttataa
     1741 attttaatag ttctttgtga ctattttgta attttagaaa tattttagta tccagggtaa
     1801 ttgtgtataa taaaacttgt atagatagat agtatgaaat taagcagata gtttaaaatt
     1861 aaaatgaaaa gaaaggaaat agttatgtta aagttgttga tagttgaaga tgatagtact
     1921 atatcttttg gaataaagta tgctttagaa caagaaggat ttagtataga tatatcaaag
     1981 gatttatcta gtggaaaaga aatgatttct tctaatgaat atagcatgat actgttggat
     2041 gttacattgc cagatggaac tggttatgag ctttgtcaat atataagagg attctctcag
     2101 gttcctataa tattcttgac tgcttgtgat gaagaagtaa atattgtaat gggtctcgat
     2161 ataggtggag atgattatat aacaaaacct tttagaatta gagaattaat atccagaata
     2221 aaagctgttt taagaagaaa aggtaatact tcagaagaaa ataagaaaat acttaaattt
     2281 ggagatttaa gtatatatac attagaagct agagtatata aaaatgataa agaaatattt
     2341 ctgacatcag ttgaatataa acttctttta attcttatac agaataaaaa tactgtttta
     2401 agtagaactc aaatattgga aaaactttgg gatgtaactt atgattttgt aaacgacaat
     2461 actctaactg tttatataaa aaggcttaga gaaaaaatag gtgatgattt atgtgaacca
     2521 atctacatag aaactgtaag ggggattggt tataaatgga taggaagtga aaatagtgtt
     2581 tctatataat ccagaagtga agaagttttt aagtaagtat gttactttga tgtttattgt
     2641 tatcataata tctattggat ttagcataat caacgtgtcc ttaactaaag agatggttgt
     2701 aagaaataat caggctataa taggaacttt atcaagcaag tatccaaact tggaaagtca
     2761 gatagtagac ataataactc aagggaagtc tatggagaac atagattatg gtcagaaaat
     2821 attaattaag tataattatg ataaatctat taggataaac tctgagccta ttatttctaa
     2881 attagtttta gagactatca aaataaatat aatattagta tgtataatat ttattttgat
     2941 atttgtgttg gttgttagat attttaagtc aatttacaat gatttatcag atatgactaa
     3001 gtatgtttac tatagttcag agggtaagcc atttgatatg aaaaacaaga atcaagaagg
     3061 gcagataggt cttctaaaaa ctgaactctt aaagatgaca acaattctta atgaaaaggt
     3121 tgaactttta aaaagagaaa agatattttt aaacaataca atttccgata tatcacatca
     3181 attaaaaact cctatgactt ctttaataat gttgaatgat ttattatata atgatgtacc
     3241 atacgaagta aaaatcgatt ttttaaataa gataaaaaat caattaaata gaatggattg
     3301 gttgattaaa agcatgctta aattatcaaa ggtagaagca aaagtgataa attttaaaaa
     3361 agataaggta aaatttagtg aactcataca tagagcaatg ctatctatga aaatacctat
     3421 ggagattaaa aatcaaaaac tgactataga gggaaatgat aatgtatctt atattggtga
     3481 tattgattgg tccgtggaag cattagtgaa tataatcaaa aattgtgtag aacatactcc
     3541 tgaatttgga aatataatta taacctataa agaaaatcca ttattttctg aactaataat
     3601 aaaagatgag ggtgaaggta tagataaaaa agatatacca catgtattta aacgattcta
     3661 tagaggaaga agtagttcaa aagaagatag tgtaggaata ggtcttgcaa tgtcaaaatc
     3721 tataatagaa agccaaaatg gcgatatata tgtaaacagc gaaaaaggtc agggtacaga
     3781 atttcatata atatttcata aaatgtatga tagtgactaa tctgtaatat ttaattcact
     3841 ttatagtaat agccaaaacc taatatatga agtatagaat ataaatattg gaggtagtta
     3901 tggaaatttt aagagtagaa aatctaacta aaagttatgg taaaaatgaa acaaaagttg
     3961 atgctataaa aaatgtaagt ttatcagttg aaaaaggaac gtttatagca ataacaggtc
     4021 caagtggaag tggtaaaagt accttattac atttattagg aggagttgac agaccaacta
     4081 gtggtaaggt ttatataaat gatgttgata tatataattt aaaaactaag gatttggcga
     4141 tatttagaag aagaaatata ggtcttatat atcaatttta caacttaatt ccagtcttaa
     4201 atgtgaagga aaacatattg cttccagcag agcttgacaa tagagatatt gatagagaat
     4261 attttgatga tttaatgaaa acattaggtc taattgatag agaaaagcac cttccaaacc
     4321 aattatcagg aggtcaacaa cagagaacct ctataggacg tgcattaatt aatagacctt
     4381 ctataatatt agcagatgag ccaacaggta atttagatag taaaaattca aaggaaatat
     4441 tagaattgtt aaaattgtca gtaaaaaaat acaaccagac actaataatg ataactcatg
     4501 acaaaaatat ggcacttcaa gctgatagaa ttataagcat agaagatgga atcataaaaa
     4561 gtgatgaggt gatgtagatg aatttatata cattactaac cattagatac ttaaaacaaa
     4621 ataaaagaag aaccattgta accataatcg gtataatact ttcaacagct ttaatttgtg
     4681 gtataggaaa tatatttgaa agttttatgg attatcaaat gagagaaact ataaagaatg
     4741 atggaagttt ccatgttaca ttttatgatg taaatagaaa aaatgtggaa tatgtaacga
     4801 aatcagctga aatagaaaaa catgcattta ccaaacaact tggatattct aaattagaaa
     4861 atagtgaaaa tgctatatta agtataaagc aatatgataa aaatgcaata aatggatata
     4921 aagtttctat aaaagaaggt agatttccag ctaaggaagg agaaattgtt ctaagtgaaa
     4981 gtattattaa tcttatagat gataagttaa aaataggaga taagataact ttaaaagtag
     5041 gggatatgtt tgactcaagc aagaaaaaga ttgatagtat gacatttcat gaaggtgatt
     5101 atatagcaaa tgaaaaagat agaactttta aagttgttgg tataattgaa aaacctgggt
     5161 ttgaacgtta taatggaatt gcaacagcta tagcctactt taatcctatg aataattaca
     5221 atgatacaat taatgtatca gtagcagtaa gaaatcctaa agatgtgtat aagataagta
     5281 ataaaataag taaaaatata tattcaaata aagataaaga agcagtttct gatatgataa
     5341 aatacaatga acatttattg aggttacaag gtgctagtaa gtattcaaat ataaataact
     5401 ctataaagtc tataattata gcagtaacta ttttagtaat catatgtaca attgctacag
     5461 tgtacaattc ctttagtatt tctataaatg aacgaaaaaa acagtttggg atattaaatt
     5521 ctataggtgc tacatctagt caaattaaaa gattagtttt tatagaaggt attataatta
     5581 gtttaattgg tataccaata ggactaatat ctggaacagt agctatagac ctattgttta
     5641 aaattataaa taagtatttt actgaatcgg ttgttactaa gatgagtttg cagatagtgt
     5701 ataatccaat aataactatt gttagtataa taatagtttt gcttacaatc tttatatcaa
     5761 ttctactacc tgcaatatcg gcttctaata tttctcacct taatgtgatt aaaaatactg
     5821 gagagtataa agtgggcaaa atcaaaagct caagacttat taaaatgatt tttaaaactg
     5881 aaggagtact tgcatataaa aatattagga gaaatagaaa aaaatttata ataactcttt
     5941 tttctttaat ggtaagtata ataatattta tatcttttag tggctttaca ttacttttac
     6001 taagaggtga agaaattaga aattctcaaa gaaaatatga tttgtattta actgcaaaga
     6061 acacagctag gtctgtagag gatactataa gtgaactaga agatattgat gggataaaaa
     6121 actttgaatt ggcaacagga caatatgtgt ctataagagt aagtgaaaat aaaataaata
     6181 aatccaagga agatttaatc aggaaatatt atcaaaaata taagattgga gatagttatg
     6241 aatatgattt cagtaataat gaattaattt ttccaggaga ttttgcagta aaaaacaaaa
     6301 taaataactt agtacagggc tcttttaata aagagagagc aattgaagaa aatggtgtta
     6361 tattagttag aaaaagtgca tttgaagaaa atgggaagaa aggtgtagta gaacttacca
     6421 actataaagt aggagataca gtaaattgtg aatatcttga tgaaaatgcc ttaagtaaaa
     6481 aagtaaaggt caagattctt gctataactg acgaagaacg tctaggtctt ggttatcaaa
     6541 atatggggtt acaatttata acttatgatg aagttgctaa aaatttaaat ttaaaattaa
     6601 gtaaaagcct tatatttatt gattcaggag gaagcattaa gactaagaag gaaatagaag
     6661 ctttggcaaa taaaaataat tttaactttt atgatgaaag tgcaagcaat gaaagtgaaa
     6721 aacaagattt aaaggttatt aaaatatttg tatatggttt tatagtagtg atatctttag
     6781 tgagtgttac aaatattcta aatacagtaa gtacaagtat aaatctaaga aaaagagaac
     6841 ttgcagtaat acaatctata ggggttactc ctaaaggatt taggaaaatg atatatttag
     6901 aaagttttat atatggtata ctgtcattgt tgtttggaat accaattagt attggtataa
     6961 ttctaattat gaataaactt atttcaggtg taattgaatt ttctccgatt ataccgtgga
     7021 cagccatagt tatctgtata gtaagtgttt ttataataac ttttatagca gggtatatac
     7081 caatgagtaa attaaataaa gaaaatataa tagataatat aagaagagaa agtatataga
     7141 tataaaaata attcttaact atataaaata ttattatatc acttaaatga ttttttattt
     7201 atataagaaa tctaataata aattactaga ggtatttaac tatttagaaa gaattggtat
     7261 tatataaagt aagagaatat ataataagaa agaatatttg attttattgg aaaacaaaag
     7321 aaaaagaagg tgattactat ttctcatata actgcgaaga atatgtataa aagtttagag
     7381 gaaagaataa acaagtttcc acaaggtgct ccaccatctg atacattata caaaatacta
     7441 aatgtactat atacagaaca agaagcgaag ttagttgctc agcttccaat aaagcctttc
     7501 agagtaaaaa cagcagctaa aatttggagt gtaagtgaaa gtgaagcata tagagtattg
     7561 gataaacttg ctagtaaggc tctgatacta gatatagaag ataataaagg gaagaaatat
     7621 ataatgcctc ctccaatggc tggttttttt gagtttgcca tgatgagaac gagacacgat
     7681 atagaccaaa aacttttggc agagctttac tatcaatata tgaatgtaga agaagatttt
     7741 atcaaagatt tattttattc aacagaaacg aagcttggaa gagtatacgt tcaagaggaa
     7801 gttttaacta atgataatga ggttagtatt ttagattatg aaagggctac tcatataatt
     7861 gatgaatcaa ctcatatagg tataagtatg tgttactgta gacatagaat gcaacatgtt
     7921 ggaaaagctt gtgatgctcc tatggatata tgtatgacgt ttgacaatgt agcaaattct
     7981 ttaataaata ataaatttgc aagacgtgtg gataaaatag aatgtaaaga actacttcat
     8041 caagcatatg aacataactt agttcaatgt ggagaaaatg ttaggaaagg ggttacgttt
     8101 atatgtaatt gctgtggatg ttgctgtgaa gcaatggtag cagctaaaag atttggaaat
     8161 cttcatccag tacagacaac aagttttata ccaaatataa accatgaaaa ttgtgtaaag
     8221 tgtggtaaat gtataactgc atgtccaata gatgcgataa gcaaggttaa ggaagatggt
     8281 aaagaatata taaaaattga tgaggataga tgtctaggtt gtggagtttg tgttagaaat
     8341 tgccataaaa atagcattat gttattaaag agagatgaaa aaataataac tccagcaaat
     8401 tcagtacata gagcagttct tatggcaatt gaaaaagggc agttacaaaa tttaattttt
     8461 gataataatg ctctggctag tcatagagct atgggagcta tactgtcagc tatattaaag
     8521 cttgaaccag ctaaaaagat tttagccagt aaacaattaa agtcagtata tttagataaa
     8581 ttgttaagta tgaatgataa atagaaacta acttaaaaaa ggcaaagttt tagacttatc
     8641 tatatcttaa taaacttatc atttaatatt taaatttaat ttttacacat ataaaactta
     8701 taacttaaat aaaaaaaggc tatattcaat taatattact tgaatacagc cttttttagt
     8761 tatgttaagc tatttctttt atttgcttaa atgagttttc atttccattt ccaccaattt
     8821 gtgttacaga tttaaatctc atagttttaa atagttcttt ttgattataa tctagtgagt
     8881 ttcctactag cattacagga gatttagttt ttccagccaa aactccaaca gataatccgt
     8941 ctattaaatc atcttgttta ttcattccat ttttagcaac atataaattg tctatcttag
     9001 aatccttgta aaattctttt attacttttg cattagtttc atttctagtg ctacctgata
     9061 ttctctgtgg attttgaagt ttactttcta tatttttaga aactgtatat tctccaccga
     9121 ttacataaga tttatcaatc tttttgtttt taaacaagtt atttatttca gttatattac
     9181 tattttcatt agtaagtatt ataggcatat cattttgagc tgatacagct ccaatgctta
     9241 ctgcatcggc taatcctttt tcgccattaa ctactgaaat ctttgatata ttgctaatat
     9301 tgttcaattc tttagcaatg ttcatagaag tttgatatct atcacttcct gaaattctag
     9361 atacagagat attgtttgat tttatagtat ctaaagattt ttcatttata gaagcttctc
     9421 caccaactac atatacattt ttaactttta atctctttaa ttcagccaaa gttttttcat
     9481 ttatttgaga gcttccagtc aataatatag gagcgttttt cttgtatgca aatggtgttg
     9541 cagccaaagc atctgcaatt gctgaatcgt ttattaaaac tgcattttca gcattttccc
     9601 aacctgcttg acttatctta atagaagttt catatctatc agttcctgta agtgcacttg
     9661 catttacttc atcttgatat gtaacctcaa atctagcatt tggacttcct aagaaaacaa
     9721 ttacgcctcc ttttggcaat ctaactctat tgttctttgt aactaaagaa tagtttacag
     9781 gaacaccttt atcatcataa acagcaaaag ctgaattttg aggaagatta acttctattt
     9841 ttttatttgc gatatcgtca ccaatcttat accattttgc ataaccattg gattctagtt
     9901 cacaagtaaa agatttttct gctggcaaat tagttataga atcttcacta acatacgtta
     9961 gtgttccaaa agataaatac tctgtaccat tttctttatg aagtttaatg tcggataaat
    10021 cacgacctat tacaccaggt atttctataa aagcatttga gttattctca tccattattt
    10081 tagtgttaac tatataacct ggagtttcat cagaaagact aaaacttgct tttacagagc
    10141 caaacatata gctttgagaa gtatattttt catccactag atagtaacct ttgccacttc
    10201 tttttttcca cacttctttg acactatttg atatattgtt gtcatctact ttttgagcta
    10261 catagtataa acttgctgtc tgacctaatc cagggacatc atcataagag ctcatattta
    10321 aatatgtgat attatttttt tcttttacaa attttaaaca actattacct ttttcagata
    10381 caaatctatc ttgacctatg tatacgtatt tatcttcagg tccattttct atataaggag
    10441 aagatactgt taaagtacca ttatcattaa catctacttt tatcatattt gaagaagcat
    10501 aaagaccact attttctttt aaagagctag gcattttaac ttgttgtggc ttactaaatg
    10561 ttttatcagg ttttatttct ttaattttac ctttttcttt taatgcagat agtaagattt
    10621 cttgtccaat tatttcatta agttgactac ttccaccaga agaaagaact gcaactgcca
    10681 tattttcatc tggaagaact ataagattac tgtggaataa taaagaatct ccaccttttg
    10741 taagagcttt taaattatac tgattaaatg gataagtgtt gacacaatcc caccctaaac
    10801 catatccaag tatagagtct tctccttcag gccatagacc atttaaatat tctttgtttt
    10861 ccatagcttt tactgaagca ggagaaagaa taccatttga atttttcata aatgtttgtg
    10921 caaatgtaca tagattttct gcacttgaat ataaaccacc tgcacctata gcattaaggt
    10981 tatcttgtgg aactgcatct tcccaataag gtacatatgc ttttgctagt tttgaactat
    11041 caaaactatt ttctggagtt tttgtatttt gtaagttaag tggattattt atgtatttat
    11101 caagaaaatt agtaaatgac ataccagata ctctttctac taaaatttca gctaaagtaa
    11161 atccatcatt acaataaaca gaaaaagctc ctggtttagc ttttaatctt tgtttttgta
    11221 attcttttaa gaaattatca tgaccataag aatcattgtc tgctaataaa atagtgtttt
    11281 taaaactact tcccatcaac cctgatgagt gatttaataa cattcttgga gtgatttcct
    11341 tgtatctatc atcagccatt ttaaattcag gtatatactt tacaacagga gtatctaaat
    11401 ttaattttcc atcatcaacc aacttcataa cagctgttgt agtaaacatt ttactgatag
    11461 atgcgatact atacatatta tccttattta agtttttgtt atcctgtttg ctgtaaactc
    11521 caccattacc agaaatctca attttgccat tatcaatcaa agcatattga gcacttacag
    11581 caccatactt tgatagcaaa gtttggattt tctctttagc aagagcttta gtttttgtgt
    11641 aagattccgc gtttttacta taatcatgta attctatatc agaactttga tatttttcgg
    11701 aattatcttt tgtattatca gcaaaagtaa tatttggagc agcagtaaat aacatactca
    11761 atactaatgt actagataat aatttagaga gcctttgctt aaacatatta cattacctct
    11821 ttcccttttt aataatttat aattagcata acattcttaa ttattttaat caacagatta
    11881 ttatcaataa aaaaagcttt tataagtata gctttttata taacagagaa aaatataaaa
    11941 ataatatttg ttgttgtata aagaataaag ggtataggta atatataaat aaaatggagg
    12001 aattttagac atatgtataa gaatataaat aagaagcaac ttaaagaaat gatgaaaaat
    12061 gaaaaggatg ttttattatt agatgtaaga actaaagatg aatttaagga atataaaata
    12121 gaaaatgcta taaatatatc tttacaagag ttaatcaata atatagatga aatttacgat
    12181 tataaagata agaaagtagt agtttattgc agaagtggtc atagaagtgt tactgcatgt
    12241 aatttgttag ctgaagaagg ttttgagcat ttatataatt taaattcagg aataattgat
    12301 tatatgagtt aaataatgta agttatacat taaatactat gtatagctct gcttattaag
    12361 taggaatata tctaaatatt tataagataa atttaggaaa gaacgaattt tttcgttttt
    12421 ttctatgaca gtttttgtag taaatattag tttttaagtt ggaaaaaggt agtagctttt
    12481 tagctactac cttttaatat gaacttagaa tattattaaa aaaatatatt taaagacaga
    12541 attaattttt taatgaccag ttaaattact atcgccgaat ttatcattaa atggttttat
    12601 aactctattt ataggtcctt ttagaagtac atttaaacaa atagacagaa tcataaatag
    12661 taatagaact agtatatctt tagttagatt tggagcatat ataccaccaa ctgcttccct
    12721 tgtagcacct attgcatagg taaatggcat tatagggttt attctttgga agaatgtagg
    12781 tgtaacttgt atagggaatg taccaccaga agaaccaatc tgaacgacta atagtactat
    12841 tgcaattgct tttcctacat ttccaaatac agaaactaaa gaatacacta taaagttaaa
    12901 taccatacta gtaaacataa gcagaactat caataaaatt ggatgttttg ctgttactcc
    12961 taacaataat atatctccag ttcctattat aaaaccttgg acaagagcta tacttaaaaa
    13021 agtaaggcct cttccaaagt aaatttcata aggtttatag tttccatgta cagatgtaga
    13081 caatagtgct gaaataagta atataccaac ccatgtagaa agaacactat aaaatggtgt
    13141 cattgcagaa ccataatttt caattggata taatttctct gttttcacct caacaggttc
    13201 ttttaagaaa ttagactttg tcaatatatc actttcaagc aagctgacta attttttcat
    13261 atcttcacca ttactaattt tgcttaaagt atcaatcaaa tcatccaaca ttccttttgc
    13321 taaaggaagt ctttctctta tcaatgatat actttcctgt gcatttcctg aaagttttag
    13381 agaagtagta agtatttctt caacctttgg aagttttgct tctgccttat ctaaaacagt
    13441 aattatatca tttgctactt ttatactgtt tgcaaatata ctatttatag gttttgatat
    13501 ttttgagtca aaattattta aaatatttaa attaattctt ccaataccat ttgatagact
    13561 taaaacatta ttcaaagcac ttatagatgg ttgttgtcca gatataactt tgtttttaat
    13621 atcatttaat gttgatatag atgaatctat tttattgcta gagtcttcaa gattatctat
    13681 gacatcatcc aatctattat ttgatgtaag ttggtttaat ttagttaaaa aatccaccaa
    13741 agtatcattt aaagattgca aattagaaag tttttctgat aaattatcta tcaacttagg
    13801 tacatcttca ctgccactat ttactgcatc aattaaattt aatgtaagag aagaagcaga
    13861 agatgaaagg tcaaccatta aatttaagtc agacttaata agaggagaaa gttcgtctaa
    13921 attatcattt gtatcctcta agaatttttt taagtcactt gataatagtt ttgtatcatt
    13981 cagagtcttt ttaattgtag gtaaatcaga tttaatatct ttaactgtat cagataattt
    14041 tgaagtagca tcagaagcta aatcaacagt cttatttata tggtcaaatc tcttttgaag
    14101 gtcaataagg gaattttcaa cttttgtcaa ttttggaaga ctgtgttcaa ttcctgcacc
    14161 aactccgttt gatataccaa gaacgatttc actaactgtt tttactatag tctgattaac
    14221 ctgtagttgt attgtagaag cacctttatc tgtaatcttt ggtgcaactg catttatttt
    14281 ttcattaaca gtatagataa tttcgccttt tttaacatca tctgtaatta aagatgtcaa
    14341 atcttttgta aaattttttg gaatcttaag tgatgcataa taagtacctt ttttaacacc
    14401 ttcaagagca gttttttcat ctacaaactg ccaacctaaa ctcttgttat ctttgagttg
    14461 ttcaattaat tggtctccta cattaatctt ttgtcctgct atttcatttc ctttatcttc
    14521 atttgttact gctacaagca tattagcagt gcttccataa gggtcccaag atgcctttat
    14581 attgaaccaa gcatacagag atggtaaaaa agcaagacct aaaactatta caagtacggc
    14641 ataattgcca cgtatatctt ttaggtcact tttaaaaatt tctaatatat tttttaaacc
    14701 ctttgaaggt cttaaattct tgatattatt ccagtttaaa atttctttta aactattagt
    14761 ttttttcaaa tgtctcacct cctaaaatat tttgataaag aaaaaagtta gatttataat
    14821 caattttaaa ttatattact ttttacacaa atagtaaagt taaaattttg tcttaatttt
    14881 aatacatata tattatatat aatagtatgc tataaatata tataaatata ggaaataaaa
    14941 acaaataatt tataaaatat atggtataaa aattaataat aaatcaaata tttataattg
    15001 atataatcaa ttaaattagc cattatgata tacttttggt aatgagaaaa ttctatgaag
    15061 ggatgattga atgaggtatt ttaaaaaata cataaaaaaa tatatagtat tatttctaac
    15121 agcagtattt tttttaacat tggaagcttt ttgtgattta gctcaaccaa ctataatggc
    15181 aaaaataata gatatagggg tttcaaataa gaacttagac tacatactat caacaggagc
    15241 tataatgata ggtataactt ttttaggtgc aatatttgca ataataagaa gtgtaatctc
    15301 ggcaagagtt tctcagcatt tttcttatga tttaagacag gatgtatttg aaaagataaa
    15361 tacatactca ttaaaacaaa tagataaatt tggtagagct tcattaataa caagaattac
    15421 taatgatatc aatcaaatgc aacaattcgt taatggaatg atgagagttt ttataaagtc
    15481 accaataatg tgtataggaa gtttaattat agctataaaa ttaaatttaa agatgtcaat
    15541 aattattcta attgcagttt taattatatt tattataata ttgcttaata tgaagatagg
    15601 gtatagatta tttaaaaatg tacaagaaag tacagataaa gtaaattcaa aattaagaca
    15661 gtttttaagt ggaataaaag tagttaaact attttgtaga tacgattatg agaaccaaag
    15721 ctttgaagag ttaaatgatg aactttttaa aaatagtaga aaagctacaa ctatgatgtc
    15781 attttttaga ccgacaatta caataatcat taatgtttcc ataataataa tattactgat
    15841 tggaaggcat ttaatttatt caggagaaat taaaattggg attatagttg cttatgtaaa
    15901 ctatatgact agaatattaa catctcttat aatgatttca catgttttta atgtttttgt
    15961 tagggcaaaa gcatcttatg agagaataag tgaggttttg gttgatgaga atgaagaagt
    16021 aattgagaga gaagaaagtt tagatgttgg agaaaaagat ttaaagtttg taaactgtga
    16081 tataattttt gaaaatgtag atttttcgta taacaaaaat acagatgaaa aaatattaaa
    16141 aaacgtatcc tttaaaatag aagaaggtca aaatattggt gtgattggtc aaactggttc
    16201 aggaaagact tctttagtaa atttaattca aaaattatat ataattgata aaggtgatat
    16261 taagattgga ggacgtagta tatacagtat ttctgaagaa agtttaaggg caagaatagg
    16321 tgtagtattt caaaatagta gtgtgtttgg aaaaagtata ttggagaatg tagttatggg
    16381 aaatagagat atatgcaaag aggatgttgt aaaatcatgt gaaatagcaa atgcaaatca
    16441 atttataaca aaactcgaaa atacttatga tgaagtttta ttccaaaggg gtaaaaattt
    16501 atcaggagga cagagacaaa gaattactat agctagagct atagccaaaa aaccacaaat
    16561 attaattctt gatgattgtt ttagtgcaat ggatattaat acagagacaa ttataagaaa
    16621 gaatataaaa gagtatataa gtaatttaag agaaaaggaa agtaagaaat gtactgtaat
    16681 aactatttca caaaagatta gttctataat tgactgtgat aagattttag tcttggatga
    16741 gggtgaggtg gttggatttg ctacacatga agatttactg gttagttctc caatttataa
    16801 aaaaattttt gcgttgcaaa atttagtaag tgaagatgta aattaaggag gaagtatatt
    16861 gaatttaagt actggaaatc agaaggtcag tagattaaag aaaaaagaaa agcctaaaga
    16921 ttttaaaaaa acgtttaaaa gaatttttat gtactttaaa aaagataaga agattactag
    16981 atttattttt ttgctagtaa taattgattc aattatatca actgtaatac catatactat
    17041 tggaaaggta gttgacataa tagatatcga tacttattca aaatccattt tcttaaaagg
    17101 aataattatt ttactttcat tttatataat tgagttttca gttaaactgt ctaagggaat
    17161 atgtacagct agattatctc aaagtatagt cagagaaatg agaagtaatt tatttttaaa
    17221 atttaaaaat ctaccaataa ccttttttga taaaagttct actggagata ttatgagtag
    17281 agtgacaaat gatgtcgata atatcagtac aggaatatca aattcattag tacagttaat
    17341 tacaggaatc ttaaacatat tgatgacttt tattatgatg tttgtattaa gcccaatttt
    17401 aaccttttgc agtgtaatat taataccgat agtaatgttt gtgagtaatt ttattgcaaa
    17461 gagaactaga gtttttttta agcaacaaca agttgagttg ggaaaactaa atgcacatat
    17521 tgaagaaatg atatcaaaca taaatgtagt aaaatctttt aattatgaaa gaaaatctgt
    17581 aaatgatttt aaagaagtca atgatagact tctaaatatt tcccttaaat cgcaaattta
    17641 cacttctctt ttaatgccta ttatgaatgt gattagtaat ataggatttg caactatatg
    17701 tattatagga ggcattcttg catctaaaga tattataacc ataggggtta tagctagttt
    17761 tttaagttat gtaagacaat tcacaagacc tttaaatgaa ttagcaaatc tatttaatac
    17821 attactttca gccatagctg gatgtgaaag agtttttgaa attatagatg aacaagaaga
    17881 gcaagaagtg cataacatgt ttaaaagtga gattaacgat aataaagatg gtaatttaaa
    17941 ctctcatttt gtaaagggag aaattgagct taaagatgta agtttttctt atgattcaaa
    18001 aaagaaaatt ttagataata taaacctaaa aatcaaatct ggcacatcaa atgccatcat
    18061 aggagagacg ggaagtggaa aaacaactat tataaatcta attacaaatt tttatcatat
    18121 agatgaagga agtatatttt tagatggtaa ggatatatac tctattaaca gaaactattt
    18181 gagaacttgt tttggagtag ttccacaaga aggttatata ttcaatgata cagttatgga
    18241 aaatataagg tatggaaatc tggaggctac tgatgaagaa gttatatatg ctagtaaaat
    18301 agcaaatgca cataaattta tagttaaaat gcgtgaagga tatagtactg tattatcgga
    18361 taggggtgaa gatttaagtg aaggtcaaaa acaattaata agtattgcta gagcagtatt
    18421 aaaaaatgct ccaatactta ttttggatga ggctacaagt aacatagata tagcaacaga
    18481 agctaaaatt caagaagcaa taaataattt aacttatgga aagacaagta taattatagc
    18541 tcatagatta agtaccatat caaattgtga taatataata gttttagaaa atggtagaat
    18601 tgtggaatca ggaaatcata aagaattgat aaaattaaag ggacattatt atagaaacat
    18661 attattgaca cagggaaaat aagtatttag aaaaaacttt taaaaaaatt gtttaaaggt
    18721 atatggtgcg ggtatatata gactaactta ataaataaaa aatttaattt gatatataaa
    18781 atttaggagg aaagtaatta tgaagaaatt tgtttgtaca gtatgtggat atatacatga
    18841 aggagatgct gcaccagcac aatgtccagt atgtaaagtt ggagctgata aatttgaaga
    18901 aatgaaaggc gaaaaaaatt gggctgatga gcacagaata ggagtagctc aaggtgtaga
    18961 tgaagaaata atcgaaggat taagagctaa ctttgttggt gagtgtacag aagtaggaat
    19021 gtatttagca atgagtagac aagctgatag agaaggttat ccagaagtag gagaagctta
    19081 taagagaata gctcttgaag aagctgaaca tgctgctaaa tttgcagaac ttcttggaga
    19141 agttgtagtt gcagatacaa aagaaaactt aagagttaga gttgatgctg agtatggtgc
    19201 aactgatgga aaattaaaat tagctaagaa agcaaaagaa ttaggattag atgctataca
    19261 tgatacagta catgaaatgt gtaaagatga agctagacat ggtaaagcat tcttaggatt
    19321 attaaataga tattttggaa aataaattta atataaagta gattttagga gtatctcact
    19381 tacaattttt aaagttatga gtgagatatt ttttaattat ttgtacttta ttttttattt
    19441 ctaacgatta tatttttaat aatagagaat aatttaatag taataatgca caacttttaa
    19501 aaataaatga ttaatccaaa aataaatttg attttagaaa agtaaattga catgtaaaga
    19561 agaatatatt aaactaagta taaaaaattt gctgttttgt aaaggtggtg gataatctga
    19621 taaagtatat acataaaagt tatgtagcta ttgttgtttt acatgaaatc tatgggatta
    19681 atagctttat agaagatgtt tgtcaaaagt attataaata tggatatgat gtattttgtc
    19741 ctgaacttct gggagaaaaa agagtttttc catattcaaa agtccaagag gcatatgatt
    19801 tttttataaa taatgttggt tttgatattt ataaaaaagt tgaaaagctt ataaatgatt
    19861 taaaatcaga gtataaatat gtatttgttg ctgggtatag tgtgggagca actattgcat
    19921 ggagatgcag tgaagattct ttttgtgatg gcataatttg ttgttatggc tctcggattc
    19981 gagattacat ggatgtaatt ccaaaatgtc ctaccttatt agtatttgca aagcacgatt
    20041 catttgatgt tgattctatt tcttaccaac tttctaggaa acaaaatatc caaattgaga
    20101 ttttagatgc aaatcatggt tttatagata gatattcaaa taattattct gatattcaaa
    20161 caaaagtatt tactattaaa gaaaaaagtt ttctgtctaa atatttagca tgatttagat
    20221 tattataaaa tatatagaaa gatttaatta atctgaaaag tttttatgac actattgata
    20281 tttaaaatat tagtagtgtt attatatttt ttgaaataaa attaatcact agaattatag
    20341 tgttagttgt gcttttatga cttataagaa actaatatta gatagtgtat ttaaatagat
    20401 aataataaat tcattatagt tgaataagaa tattatatga gaatattgca atttgatata
    20461 tttaccatta caataaaagt agttacaact agaaaaccaa ataagttaaa gtaagttggc
    20521 aaattggagg tttttaaata tgaaagcatt taaaaagcac ttcttgatac tttctttgat
    20581 atttttactt attatatcat gttttaacaa tatactatgc tttgctgatg agtctgaaaa
    20641 ttcaaaaaaa ataagagttg gatattgtga tgactacgga ataatatcct catcaaaaga
    20701 gcattcttat acaggatatg gttatgatta tctaagagaa atctcaaaat atacaagatg
    20761 ggaatatgaa tttgtaaaag ggtcttggga agaatgttta gacagactag aaaaaggtga
    20821 aatagactta cttggacctt tacagaagaa tgatgaaaga aataaactat ttaattttcc
    20881 taaactaaat tcaggatatg aatatagtgt actatataca aaagatagta acaataatat
    20941 gttttatgaa gatataagtt cctttaaggg aatgagagta gctgtactta gaggaaactt
    21001 tcataatatt gcatttgaga agtatagaga agaaaataat ttttcagttg aatatatcta
    21061 ctgtaattct attgatgaat taattgagtc tgtcaatgaa aaaaaggcag atgcatttgt
    21121 ctgtggaagt attataaatg ctaaaggaat gaaaatagtt agcaaatttt cagtagagcc
    21181 tttttatttt gctactgcaa aatataaacc taatttggta aaagaattag attatgcttt
    21241 aaaagaatta aaaataaatg atatgtatta tgagttggaa ttatataaaa aatatttcaa
    21301 aagagaggtc aataattcca tagcatttac tcggcaagaa atgaatttta ttaaagcaaa
    21361 cccaaaactt actatggtat atgattcaga gtggtctcct gtagagtatt atgataagga
    21421 aagcaattcg tttaaaggta taagttccaa tttaatgtca attatatcaa aaaaatgtgg
    21481 tataaaattt gaatatatta aaacaaaaaa ttataatgag agtcttgatt acataaaaag
    21541 tggtaaggca aatatgctat gtggctcaat caatgaaaat gataaggcaa aacgatttaa
    21601 tatgaaattg acaaatccat acataaatat tccaatgatt atggtaggaa aactggatac
    21661 caatctcaat aatgatttaa atattgcttt aacgagtagt tataagtcta tcgattctta
    21721 tatagaaaaa agttttgaaa atgctaagac aacatcttat aagagtatag aaagttgtct
    21781 aaacgctata aatgaaggta aagcaaactt aatgatactt agttcttatc agtttgatga
    21841 acttttaaga gaaggaaaga gtgagaattt atctgttata tctgttttag atacaagcta
    21901 tgggatgcgt ataggggtat caaataaaac tgaccctata ttagtatcta ttttaaataa
    21961 atcaatagac aaaattactg aagaagaatt aagtgattgt atatattcta atactattga
    22021 caaaccttat aaagtaccat ttggtgtgat ttttaaagaa tattcaatac aaattatttc
    22081 ttttgtatgt attttattct taattgctat taaatatata atttataata aaaagaagaa
    22141 agaggattat ctaaagaaga tagcatatac agacccattg acaggagcag atagtataga
    22201 taaatttaaa ataaattcga ataaactgtt tgctaaaaat aatccagaag aatatgcatt
    22261 gttttatata gatgttgaca aatttaaata tataaatgat atgtttggat atgatatggg
    22321 aaatgatact ttgatacata tatcaaatac tattgcaagt gaattaaagg aagatgaaat
    22381 ttttgcaaga gtttctgctg accattttgt tcttcttatt aagtataaga cagatgatga
    22441 tattaaaaca aggttaaaca atatttacaa taaggttcaa atacttagta atcctaaaat
    22501 aaactattat aaattgattt tagattgtgg tatatataaa ataagtaaaa gtgataatga
    22561 cataaatact attatggata gagctaatac tgcaagaaaa actataaaag gtggtcataa
    22621 aaattcattt gctttttacg ataaggaaat gcataaaaaa atattaaaag agaaagaaat
    22681 tgaaaattca atggtagatg ctcttaataa tggagaattt attgtttatt ttcaacctaa
    22741 atatagtctt tcagattatc aaattatagg ggcagaggct cttgttcgat gggataatcc
    22801 acaaaaaggt ctaataccac caatagaatt tattcctgta tttgagagga atggttttat
    22861 tgtaaatatt gatttctatg tatttgagga agtgtgtaaa aagattagag aatggatgga
    22921 tgaaggacaa aaagtagtac ctatatctgt aaacttgtct cgaatgcatt ttgtaaatag
    22981 taattttata gagaagttta aattaattgt agataaatac aaaattccga caagacttat
    23041 agaattagag ctaacagaaa cagcagtatt agataatata gaagggttac ttgatacaat
    23101 gaacaatttg aaagaaaagg gatttgtaat ctctatggat gattttggta cgggatattc
    23161 ctctcttaat cttttaaaag aattgcctgt tgacatatta aaactagata gagcattttt
    23221 tacagaaaaa gatgagagta ataatgaaaa aattgttata tctaatgtca ttaaaatggc
    23281 aaaggaattg aaaatgaaag taatatcaga aggtgtagag actatttcgc aagtagaatt
    23341 tctaaagcaa attgggtgtg acatggtaca aggatatttg ttttctaagc caatgcctgt
    23401 aaaagagttt gaaaaaattg cttttaaaaa agaataaagc aatgtgtaag taatgatata
    23461 tataataaag gtgctttaat attagttgag tatactattt tagagcatct ttatttttgt
    23521 attaattttt acgattttat taataaaata aatcaaatga gaaaaaaaat caaaagaata
    23581 caataatcac ttgcaaaaaa tatactattg tgatagactt aaatcaaaat taggaattgt
    23641 aaaaagaagg aaaaaatagg atattatttt aatagattaa gatttaaggt attgtaattt
    23701 atatataaga taacgattgc ttgtagattt aaattagttt aagaggaggg atattatatg
    23761 atgccattga tactatcaaa tacaggtgaa gaagtagtta taaagaaaat ttcaggaagt
    23821 gataagacga ggactttttt aaatagtata ggttttgtag taggtacaaa tgttaaaata
    23881 gtttctaaga tagatggtaa cttaataata gacgtaaaag atactagagt tgcattagat
    23941 aaaaaaatgg caagtagaat aataatatag ggaggtaatg acatgaacag actgaaggat
    24001 attaagtgtg gagagactgt aaaagtcaaa aagttagaag gagaaggagc tacacgaaga
    24061 cgtataatgg atatggggat tacaagaggc gttaatatat ttataagaaa agtagctcct
    24121 cttggtgacc caattgaggt aacagtcaga gattatgaac tatcaattcg taagtcagat
    24181 gcagaaaaaa ttattgtgga ataataaaga ttaattagag tagagttaat gtaattatat
    24241 aatgagaatg attttctata tcatgaatag tgatgtattt aaataataat ggagggaatg
    24301 gaaatgtcaa taaaaatagg tctcatagga aatcctaact gtggtaagac aacaatgttc
    24361 aatgggctta caggctcatc acaatatgtt ggtaactggc caggagtaac agttgaaaaa
    24421 aaaggtggaa aattaaaagg aaataaagat gtggaaatag tagatttacc aggtatttat
    24481 tctttatctc catatacatt agaagaagta gttacacgta attttatgct agatgataag
    24541 ccggatgcag taataaatat tgtagatgca tcaaatatag agagaaattt atatcttaca
    24601 actcaagttt tagagctagg tataccgacc gttatagctc ttaatatgat ggatattgta
    24661 aataagaatg gtgataagat aaatattaaa gaactatctg aagttatagg ttgcccagtg
    24721 gtagaagtaa cagcagttaa aggtcaagga attatggagg ctgctgaaaa ggcagttgaa
    24781 cttgcaagta gtaacaataa attaaatttc aaattaccct ttgttgatga aagtaaggat
    24841 gcaatagaaa aaattgaaaa aataatagaa gaaaagactc catatataga tgtagaaaca
    24901 cgctggttag ctataaagct atttgagaga gatgaaaatg ttatacaaaa acttgatatt
    24961 tctaaaacaa tattgaatag tattgaagaa ataactagaa attgtgaaga tgaattggat
    25021 gatgatagtg aaagtataat aacagccaat cgttatgaat ttattagttc aataatttca
    25081 agtataataa agaagaatcg aaaaggtaag gaaactgtat cagataagat agataagatt
    25141 gtaacaaatc gtatactagc attacctatt tttgcactta taatgtgggg agtttactat
    25201 atagcagtaa gttcattggg tactatagca actgattgga caaatgatgt actgtttgga
    25261 gaaattattc aaggcaatgt aagtaatttt ctagcttctt taaatgtagc agagtggtta
    25321 caaggacttg tagttgatgg tctaattggt ggagttggag cagtactagg atttgtacca
    25381 caaatcatgc ttttattctt attattatca atattagaag attgtgggta catgtcacgt
    25441 gtagctttta taatggatag aattttccgt aagtttggtc tttcaggaaa atcatttata
    25501 ccaatgctta taagttcagg ttgtggagtt ccaggagtta tgtcaactcg tactatagag
    25561 aatgatagag atagaaaaat gactattatg ttaactacat tcattccatg tggagctaag
    25621 attccaataa tagcattatt tgcaggggca ttatttggag gtgcttcatg ggtagctcca
    25681 tcaatgtatt tcttaggtat tgcaatgata atcatttgtg gaattatatt aaagaaaact
    25741 agtttatttg caggagaacc atctccattt gttatggaat tacctcaata tcatattcca
    25801 agtgcaaaag gtgttttaat acatatgtgg gatagaggaa aagcatttat aatcaaagca
    25861 ggaacaatta tatttgttgc ttgtggagta atctggttct tacaatcatt taattggtca
    25921 ttacaaatgg tagatgctgg agatagtata ttagctagct taggaaatat agttgcacca
    25981 atatttgctc ctcttggatt tggaaattgg caatcatcag tagcaacagt tacaggtcta
    26041 gtagctaagg aaaatgtagt tggaacattt ggagttttat ttggtatatc agatgcaaca
    26101 gagcaagacc caacacttct tgcttcagtt gcaagcatgt ttacagtagc tagtgcgttt
    26161 gcatttatgg catttaatat gctttgtgca ccttgttttg cagctattgg agccataaaa
    26221 agagaaatgg gttcatggaa atggacatgg attacattag gattccaaac tttaacagcg
    26281 tatataattg ctcttcttat aaatcaagtt ggaagtttag ttttaggtac tggaggaagt
    26341 attgctgggg caataatatc tatatttata gcagtagcag tagtctttgt agttttaact
    26401 tattcaaata aaaatatgaa aaaagaaaaa atgggtaaat taagttatat gaaaaattaa
    26461 ttcatataaa taaggagtga tgaatatggc tacatttatt atagcagcta ttgtagtagt
    26521 attaatggca ctagctgtaa tgtatatggt taaaaattca aaaaagaatg gtagcagttg
    26581 tggatgtggt tgttctggat gctcaagtag taagagttgt catagcgcta agagataaat
    26641 aggataagag cctgataaaa agttaaattg ctttattatc aggttcttat tttttgtata
    26701 aataacaaat ttcatatttg aagtaggtta gactatgctg taattttact gtataattat
    26761 atataaatat atgattagga gggattattg ctaaattaag caatataagt gaatattatg
    26821 tttaaatttg atgttacaaa cgagcttatg tgtatagcaa ggggaagtgg taaatttttt
    26881 gcaaaaaaag gtgctatggt agcctttaaa ggaaatttta attttgaaaa attgctgtta
    26941 ggtccaagta atggcggtgg attgggaaga gcattattag gtcatgttca gaggtcattg
    27001 acaggagaac aaatgccaat aatggtggtt gaaggtgaag gtgaaattta cctagctcaa
    27061 aatgcttacc atgtagatgt aatatcaatt gacccaggtg attccattta tgttgagagt
    27121 gaaaacttat tggcattttc tgaacaatta aattacagtg taaaattgat tggttcaggg
    27181 gtaatatctc aaaaaggtct ttttactaca catttagtaa ataaaacagg tcaaaatcaa
    27241 gatgttgcta taataacaga tggtaatcct ctaattcttg aaggaccttg ttgtgttgac
    27301 ccagatgcat tagtagcttg gactggaaga gaaccatatc ctaaagtatc aaaactatca
    27361 tggaagactt ttattggtca gacttcagga gaatcttatc atatggaatt tgttgaacct
    27421 ggacaaatag tcattataca accatctgaa agattgtctg gcttaaaaac agattctaca
    27481 ccttctgata gaatgagttc aggtggatta agacttggca tagattaaat cttatgatat
    27541 agcttaatta ttatttaaga tattaaaaaa taaaaagggt agcagggata gatatgttaa
    27601 aaggagtttt atatgtttga gaaaattcat aaaaggctta aaggatatta tattttttta
    27661 ataattttaa ttttatggaa gatagtatgt gttttgggta tatggagtag ctatattctg
    27721 cctcctcctg aaatagtctt agatactttt ataaaaatga tatatgacgg ttctattttc
    27781 ttaaatgttt atgccagtgt aagaaggata ttaataggtt ttttaataag ttcattgatg
    27841 gctataccac ttggagtttt atttggagta aataaaaaaa tgtatgaata tttcaaacca
    27901 ctatttgaat ttatgcgaag tacacctcca ctagctttgg tgccaatgct tattttatgg
    27961 tttggtatag gtgaagaatc taaaattata attattattt tggcatcctt ttttccaata
    28021 tttttaaata cattgaaagg tataaaaaat tgtgacaata aattaataga agtaggtaaa
    28081 tcttttaatt tatctaaaaa gagaattttt tataaaataa taatccctaa ttctatgctg
    28141 gatattgttg ttggacttag tttagggctt gggtatagtt ttagggctat aattggagca
    28201 gagcttattg cagcttcttc aggactagga tatttgattt ctgatggaaa agatatgtct
    28261 agaactgatg ttgtattggt aggaattttt gttattggat ttttaggtat actttcagat
    28321 tatctatttt caaagataat aacaaaattt agcaaaggga agcaggttga tttaaatggt
    28381 tagaagtgga tttttgctag agaatatata taagaaatat ttagttgata ataaagagca
    28441 tttagttctt gataatatat ctttaaatat atctagtgaa gagataactg ttatattagg
    28501 tgaaagtgga tgtgggaaaa caacgctact tagaatttta gcagggcttg aaaatgctac
    28561 tagtggaaac atatattttt ttaataatga taaaaagtgt actccaaaag taggaatggt
    28621 atttcaagaa agtagactta tgccatggct gaatgtaagt gaaaatatac ttttacatac
    28681 tgaaaaagat aatagaaata aagttgattt agacaagtat ttaaaaatga tgaagcttga
    28741 aaagtttaaa aactcatatc caaatgaatt atcaggagga atggctcaca gagtaagtat
    28801 agcaagagca ctttccttca atcccgatat tttgctgatg gacgaaccat ttgcagcact
    28861 agattacttc acaagaagaa aaatgcaaaa agaagttgta aatatccata aaaatactaa
    28921 aaaaggtgta gtatttgtaa ctcacaatat tgaagaagct atggaaatag caaagaaaat
    28981 aattgttttt agtaaaaata aaaggattaa acaatttagt gtcgaagatg aatataatag
    29041 agatttaact aaaaactatt atataaactt gaaaaaagaa atattaaggg aattggggga
    29101 gttttaattg attaaaagaa acaaaaagat tttagcagta atgacatctt taataatggt
    29161 gttggttggg actgtaggat gttcaacaac aaatgataag aaagatgaac ctgcaaaaaa
    29221 agaggctagt ttacctaagg aaataaattt aacttatgta aaatcaccac ttaatgttcc
    29281 tagtatatta gaaaaaaagg atgatttatt tggaaaggaa tttaaaaagg acaatataaa
    29341 agtcaatttc catgaattga caacaggacc agagcaaact caagcattag cagcaggtga
    29401 gctagacttt ttacatgcat taggaggaac gtctgcaata atagctgcat caaatggtgt
    29461 tggtcttaaa attacgaata tttatagccg ttcaccaaaa ggatatatgc ttataactaa
    29521 taatgataat attaaatcta taaaagactt aaaaggtaaa aaaattgtag gtccaaaagg
    29581 gactatactt catcaactgt tgattgctgc acttgctaaa gaaggctata ctatagatga
    29641 tgttgaatat gtaaatatgg atattcctag tgctgcttct gcattagaaa gtaaaagtgc
    29701 tgatgtagca atgcttgcag gtccagtggc attgaaaacg ataaattcag gtgccaaaat
    29761 gattgtaaat ggagaaggat tagtaagtgg aataatagtt actgctgtaa gtgatgactt
    29821 tgctgaaaaa tatcctgaat tagtaaaaag atttatgaag gttcatgaag aaactttaaa
    29881 atatatgaat gaaaacaagg atgaagtgat ggatgttgta tctaaagaag ttggtttatc
    29941 tttagatgaa acaaaagaaa tgtattcttg gtatgacttt agttctaaaa taacggacaa
    30001 ggatataaaa gaattagaag atacacagga atttttaatg tctaatggaa tgcaacaaaa
    30061 gaaaataaat attaaagata tgttgtacaa tcaaaattag aatactttaa aatattaaag
    30121 tggaaaatac acccttattt attatggaaa ctatatggta aaagagggtg tatttttatt
    30181 attatacaaa aatacaatta aaaacagctt actattttat ctacacttat gtagtctagt
    30241 aattttctca ttttcttacg gtgaagtaaa aacttcgaat atgtgtcctc ggattgtttt
    30301 gtgtcaccat aaactttcca atatttaatc atagaaactt cataattact atatcccata
    30361 aatccaatca catctttaag aggatatcct aaaaaaatac ctatttcatc aggaaaaaca
    30421 tcactcttaa gtttatctac taaatgctct aaataagcat taacattcgt attttgtttg
    30481 tatcctaaaa actttaaaaa gtttactgtt tttttacatt ttaatttttc tgataatgct
    30541 tttttattta caaataagat ttttaatccc tttttttctt tatcaataac tataaaatct
    30601 attttcttac atttatatag atatttataa atatcattaa ttttttcctc tctatttaca
    30661 tcagtaaacg aaatatttat tatttcagca ggtttagagc ctaaaaagac tggacctaaa
    30721 acttctaaaa gccattttat atatgatgat tcaattttat ttttacaaca aaattgtgaa
    30781 ttacaacaag acataaaaga taacctcctt caaatgaaaa ttaatatcaa gtaattagat
    30841 tatactatga atgataatca atatcaataa taaaattata atttttttat ttaattattt
    30901 attcattatg atttccaaaa aaataaatta taacttgttt aattttagta aataaaatat
    30961 aaaaatatag aaatactaaa ttaagaaaga aaattattaa aattttttat agtataaaac
    31021 atctaggagg gtaataaaaa tgaaattaaa aagaacttta tttgtaggat tagcactagt
    31081 attagctata ggtataactg catgtactaa aaataatgaa aaaccaagtg aaaatacagg
    31141 aaaaaatcct aatgaatctg taaataataa tgaaggatat atgaattatt attcaacaag
    31201 ctataatgat tatatagcag gtctaaatag atattctatt tatgatactc cagaaagtat
    31261 aaataatcag tttaaagata aagaatatcc aggaaatgaa aaatatctaa gtgatgtaaa
    31321 ggctgcatat aaagatagta gagataaaat acaatcattt gtaacatctc ttaaaaaaga
    31381 tggtaaaaca gaagatacag agttaaagaa aatgaatgat gacctaatcg ctgaagggga
    31441 aagattagta aaagacatag ataagagaat agaaaaatta gataaggtat ctgatgaaga
    31501 tatgaaaaaa ggtcaaaatg actttataaa attagttcat ggaactgaag atgttggaaa
    31561 tgatataggt agtggtttta gaaaaatgat aaaagatatg aatgatagat taggaataac
    31621 aggaaataat aagaaataat agaataaata gttttgaaat ttgaaataaa ttatttgaga
    31681 tatagcgaag ccttataaat aatacatttt ttggcttcgc ttttttgcat gttattaaga
    31741 tattaatttt tatcttgtaa ccttaatatt tttattataa tttatttagg gattattttc
    31801 atacgttttt acttgttttt tctcttttta aatttttgtt ttgcaaaatt gtaagattct
    31861 cctattaaag ttttaaagtt ctcaaatgtc ttttcagtag ggcttaggac gcatatccat
    31921 cccatccatg agtagatagg atgtggcatg attgtgtcca attttgtaaa atcaaaatcc
    31981 atatcaacta tttgacctac acctggacgt tttggaatat atccaaacat ttcaataaaa
    32041 gtttcttttt ttaatcctat attcacacgg tatacatttg ggcgactaac caacgaacct
    32101 ttatcatgat tactgtcttt ttctttaatg gtaagaacat atactcctcg ctttaaaata
    32161 ccattaggat tgtagtaaat tgctcgttcc ccccaactat tcattaataa aacatcatct
    32221 aagtttgata gacaataatt gataatatca tcagccttaa tagtattatt ttccataaag
    32281 tatcagtccc ttctttcttt actattatta ttgctttaaa tgtaagaata tcttatcata
    32341 taatagtgac aattattgtc actatattta aattaggagt ataaatatgt caagagctga
    32401 gcgtttaatt gaactgatga taacaataaa tgcaaaaaga agttttacag caggagaatt
    32461 agcagaagaa ttttctgtat caaaacgtac tatattaaga gacttacagg tgctggagag
    32521 tattggattt ccattatatt caaaagttgg agcggctggt ggatatcatg tacttaaaga
    32581 gcgtatactt ccaccgattg cattttcaga aagtgaggta aaatctatat tctttgcata
    32641 tcagtcacta gaatattata atgatttgcc atttgagcaa gaaactattt ctgttttaaa
    32701 aaaattttta aattgtatac caaatgatat tcaatataat attgagaata taaggcgtaa
    32761 atttgttttt tggacaccag ataggcactg ttcaacacca ttattgaaag aattgtttaa
    32821 tatagtaatg aatgaatcta caattaaaat tgaatattca tcaaagcaaa aaaatagtgt
    32881 acgtaccatt gttcctattg gtttgtatgc catgaatgga ctttggtatt gtccagctta
    32941 ctgtataaaa tcagcatcta ttaaggaatt tcgtgtagac agaattgtga agattttgag
    33001 tatagaaaac ttatcaagta agaaatataa ggttttatct tctatacatg attatcttaa
    33061 gaatatggaa gtaggtacag attatcatat taaaattaat ttgacagatg agggagtaaa
    33121 acgttgtgaa acagaatttt tgttggcaag agggttgaaa atcttgtcta aaggtggata
    33181 catagatatg tatataccaa aatctacact taactgggtg gctgaatatt ttttaacttt
    33241 tgggaaaaat gctacaataa tagaacctat agaacttaaa catttggtaa agtctaaagt
    33301 actagagcta tataatcatc attgtattta aattatttta tttaatagta ggatagagtt
    33361 tggataaaat gttaattttc ttaatttact ttacagatga tttagataga ttgatgttat
    33421 ttaatttatt tcttataatg atgttctata gttatataga cattaaaata taaagtgtta
    33481 ggagctagaa aactaattaa aaatagttaa ttaacgataa taaaaaataa aaaatttaaa
    33541 taaatttatt gacaaatact attaatgaat atatcatata taacatagca acaaatgata
    33601 atagaattaa attaaataac gtcttatcaa gagtggtgga gggactggcc ctttgaaacc
    33661 cggcaaccag tatattttat atacattggt gctaaatcct gcaactaata tagttgatag
    33721 atgagtataa atagctttct aagcctgagt ttatactcgg gcttttattt tgttgtaata
    33781 cataagagag gataaacata aaaactgagc agaatatatg ggaggaaatt ttatgattag
    33841 cataaaaaat gttaataagt attatggaaa gattcaagtg cttaaagatg taagtattga
    33901 aatagaatct ggagaaattt ttggaataat aggtcatagt ggtgcaggga aatcaacact
    33961 actgagatgt ataaacggat tagaagaata ccaagaaggc agtgtacttg tttctgataa
    34021 agaagtaaag tcattgaatg aaaagcaaat gagagattta agaaaagagt taggaatgat
    34081 ttttcaacac ttttcattgc ttgaaagaaa aactgtattt gataatgtag cattgccatt
    34141 agaatgtttt ggatactcaa aggcagaaat taaaaaaaga gttttagagt tattagaagt
    34201 agttggaata tctgaaaaga aaaatgataa acccagaaat ctaagtggcg gacaaaaaca
    34261 aagggttgca attgctagag ctttagcact aaatccacaa gtacttcttt gtgatgaagc
    34321 aacttcagca ttagacccaa atactacaaa gtcaattctt tcattgcttg aagatataaa
    34381 taaaaatttg ggaataacaa taatagttgt aactcatcaa atggaagtta taaagcagat
    34441 ttgtggaaga gtagcaataa tggaaaatgg agaagtatta gaggtaggag atacagaaga
    34501 aatattctta agaaatacta aaggacttag aaaattaata ggtgaagaaa gtataatatt
    34561 gccaaaagga acaaatataa agatactatt ccctaaagat atttctaatg aagctattat
    34621 tacgactatg gcaagagaac taaatataga tgtgtctata atatttggga aattagaaca
    34681 atttaaagat gatattttag gttcacttat tataaacatt tcagataaaa gtggagagca
    34741 agtaaaacaa tatctaacta gtaagggcat aagatgggag gaaatgataa atgaatagtt
    34801 taattgactt tttaacaaca ctatttccaa atgcattgtt acaaactttg tatatggtta
    34861 tagtaccaac tatagtggca acaatattag gatttatatt agcaatcata ttagtagtta
    34921 caaaaccaga tggattaaaa ccaaatagca caataaatag tgcattagga tttatagtaa
    34981 atatatttag aagtttccca tttatgatac ttatagttgc aatgattcca ataactagat
    35041 taatcgttgg aacaagtata ggggaaactg ctgcaatagt gccaataact attggggcag
    35101 caccgtttat cgctagaatt atagaaagct cattaaatga agttgataaa ggattgatag
    35161 aagcagcaaa atcttttgga gctacaaaaa gacaaatagt atttaaagtt atgataaagg
    35221 aagctatgcc ttcaatagta tctggaatta cattatcaat aatatctata cttggttata
    35281 cagctatggc aggtgcagta ggagctggag gattaggtaa tatagcactt atttatggat
    35341 atcaaagatt tgatacagca gtaatggtat atactgtaat agcacttata atattagttc
    35401 agataatcca gggagtagga aatctggctt ataagaaact aaaataaatt aattataaac
    35461 attttaaaga gggggagtaa aaatgaaatt aaagaaatta ttatcagtag cattggtgtc
    35521 agcaatagca atatcagcag taggttgttc aaacaaagag gataaaaaaa tactagtagg
    35581 agcttcatca aatccacatg caaagatatt ggaagttgca aaacctttgt taaaagaaaa
    35641 aggatatgat ttagaagtaa aaatatttga tgattatgta cttccaaata cagctttaga
    35701 tgaagggtct ttggatgcta acttctttca acatattcca tttttagagg aaactgtgaa
    35761 ggagaaagga tataaactta cttatacatc taaagttcat atagagccaa tgggattcta
    35821 ttcagaaaaa gttaagtcat tagatgaatt aaaagatgga gctgttatag ctgtgccaaa
    35881 tgatgcaact aatggagcta gagcacttaa attattagca aaaaataaat tgatagaagt
    35941 aaaagatgga gagcttataa ctaaaaaaga tataactaaa aatcctaaaa atatagtaat
    36001 aaaagaaatg aatgcagagc aattaccaac agtattaaaa gatgttgacg gagcagtaat
    36061 aaattctaat tatgctttaa cagctaactt aaatcctact aaggatgcaa tagtaataga
    36121 atcaagtgat tctccatatg taaatataat agcttgtaga gaaaataata aagatagtga
    36181 taagattaag gctttatcag aggctatgaa tagtaaagaa gttaaaaaat ttatacaaga
    36241 tgaatataaa ggaagtatag ttccagcttt ttaaactgaa tatacaaaat ctacaaaatg
    36301 agtaaaatta aaaatattta ctcatttttt tttataacta gaaagtgaca aattttttgg
    36361 aagaattgtg gtaacatatt tataggtaaa actatataaa aatgaagaag tggtggggaa
    36421 tatggaatct aaaggaactt taagtaagaa taaggtctat atactagcta atgggttatt
    36481 tttggttatt gcacttttaa atataaattt gtatacagtt tctataaata tgtatagaat
    36541 aattttactt tggatatttg gatattctat agtttatata agtagcttta aaacagatga
    36601 atttataaat atattgaaag tagggtgtct tgtattagtt tttgtaagta ttttaggagt
    36661 agtaaattta aaaattcaaa aattccaaat tgtattgctt aacttaagca taattaatat
    36721 gtctactttt atatacttaa ccatttctaa agtaaaaaaa ctaagaataa gcccaatact
    36781 aattttttat agtgcttttg tgatattaga ttactttata agaaatattt ttagtgatat
    36841 taactggaat atgattatta tattaattaa tgtttgtata tctatgttta atttactata
    36901 tttgttgaat aaacttgata gtaacattga gcactataag ttaataaaag atttaacata
    36961 ttttgttttt tttacaacta ttatatgttt tgtagcaatt tctaagatta atatagtaga
    37021 ttcagataga ttaataggag taatatattt actattatgt aaccatactt attatgtata
    37081 tgtatacagt cttaataata gagtagtgta tccatattat gaacttgatt caatcaataa
    37141 aaaattaagt aaaaagtcag agcaattagg aaagataaat ttagcaatag aaaaggatat
    37201 gattatacaa agaacattaa aaaactacat agaccagaga aaagaattac ttagacaagc
    37261 tctggataca ataccaaatg tatggatagt aacagactat gattttaata taagttacac
    37321 aaataataaa tttaaagatg aattttcaaa ggatatgaat aattattata aaatattaac
    37381 ttttataaag gaagatgaaa aagttgctga aaaattgaag aaatttgata acgatataag
    37441 tataattgat aaactggtag agttaaatga taaaatttat ttgttaagtt taagtaataa
    37501 taaagatgaa agcaattact taataagttt aaatgatatt acaaatgaaa tcaaaataga
    37561 tgaagaaata agaaatataa ataaagacta tgaaaacatt atactaaata ttccatctcc
    37621 tatacttgta agaagtgctg aaggtggcat aaagaagaat aaggtaataa gtataaataa
    37681 aaaatatgaa aagagtttta aatgtgtttc aagtgattta gtcgatatga cattagaaca
    37741 atattttgaa acttttaaca tagatttctt tgacaatagg gaatttaaaa gattaaatct
    37801 aacacaagaa aagaaagctg aaatagtaag ttacaatgac acagcaaact gtatagttaa
    37861 ttttgtgatg tctgactcag aaggtgaaga gcatgttgaa gaagttagag ttggagacta
    37921 ttggtcagat aataaactct ttaaattgct tacatttaga gatataacta aagagataaa
    37981 tattcttaga acagtaaatg aacaaaaaat aatatatgaa aaattactgg atgttatacc
    38041 agaggctatt tttcttgagg atttaagtac atcaagagta ctatatacaa atagagcctt
    38101 tagagagttg ttcggaattt ctagtgatgt attaggagtt acaacacaaa aatatagaaa
    38161 tatattagta aaaaaataca taaacaattt aaatatagga gaaagagaga agagtatcca
    38221 tatagtaaat gaaaataatc acattaaaga agtaaaaatg atatcaagaa cattatactt
    38281 tggacagaaa agaagtagag tgagaattat taaagattta agtgtgcaaa gagagtctga
    38341 aagacttaaa aaagcattaa taaaacaaag acagtatgac caaatgaaga tggagtttta
    38401 tgctaatata tctcatgaac ttaagacacc tttaaataat atatatagtt cagttcaact
    38461 tattgaaaac ctttataaaa aaggaaaaat aattgatttt caggatatac taaaagaaca
    38521 tataaaaata actaaacaaa atatgtttag attattgaga ttaatagata atataataaa
    38581 tatttctcaa gttaagtcag acatatataa gattaaagca gttaattttg atattattga
    38641 cataacagaa agaatagtta catctattag tagttatgct aagtctaaag gaatagattt
    38701 aatttttgat acagatgaag aaactgttat ggtagggctt gacccagaat cgatagaaag
    38761 aataatacta aatatattat caaatgctat aaaatttact ttagaaggag gagaaatcct
    38821 tgtaggtata tataaaaaag acgaaacagt agagataatt ataaaagata ctggagtggg
    38881 catagacaaa gaaaaattaa atgatatatt taatcgattt aaacaaattg aaaacagtgg
    38941 tatatcaaat gaatttggaa gtggtatagg tttatgcctt acaaaatcat tgattgaaat
    39001 tcaaaatggg aagatttaca tagatagtaa ggtaggagaa ggaactaatg taaaagttat
    39061 attcccaatt aaagaggttg ttgaagaagt atatgataat tcaaattaca atgataatat
    39121 tgaaaagttt gagatagaat tttttgatat atataaataa taaatgtcta aatttattgt
    39181 ttatatatat cacattaata agagttaatt ttatttatta ctttagttgt atttgatgcg
    39241 ttttaaataa attttatgag gtgaaatttt ggaaaaggat ttttttagaa aaaatggaat
    39301 tgatttagcc aaatcaatat taggtaaata tctaataagg aaatatgaaa ataaagtaat
    39361 tgttaccaaa atcatagaga cagaagcata catgggtgta aatgataaag gagcacatgt
    39421 atatggcaat aaaaagacag atagaactaa gccattatat ttagatggtg gacatatata
    39481 tgtttattta atttatggta tgtataattg cttgaattta tctgcaaata tagaaaatgt
    39541 gccagagtgt gtattaataa gaggggtaga gcctataact tctttagatg agatatctat
    39601 gaatagatac aacaaagctt acactgagtt gagtaaatat caagtaaaaa atattactaa
    39661 cggaccagga aaactttgca aagctttaaa aatagataga agtctaaata gtaagagtat
    39721 aatgggtgaa gaattatata taagtgattt ttattatgat gataaaggaa aaaaagtatt
    39781 tagtaaagat gagttagata taaaaactag taaacgtata aatatagatt atgcagaaga
    39841 agcaaaagat tttttatggc gtttttatat agaataagtt tttatatagg taagatatgt
    39901 atctatagga aggtatttta taggtatgta tcttattttt ttatagaatt tttagtttga
    39961 taaaaataac tttatagaat tacaaacata tttaataaat ttataattat tttcatgata
    40021 taaagaaaga tatattttgg cgaaatgagc tataaaaatt aa
//
LOCUS       DAEPIN010000029        39708 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.029, whole genome shotgun sequence.
ACCESSION   DAEPIN010000029 DAEPIN010000000
VERSION     DAEPIN010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 39708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39708
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.029"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            complement(219..2147)
                     /locus_tag="KR250_001764"
     CDS             complement(219..2147)
                     /locus_tag="KR250_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBH0067700.1"
                     /translation="MHKRLLTLFKLLNESDDKITCKTLSNHLKVSERTIRNDITSING
                     TLEKNGAIIKIKKGEGYYIDILNLALYQQYLALISDDIMDSSEIPDSPIERNQYILKY
                     ILYNNTYIKLEDLANSLYVSKFTILNDIKRIKPILSKYNLILVSKPYYGVKVEGKEID
                     IRRCISNNMINRNFENYIIGITDREIELFNNVDLIELQRVVLSEINKFNINFLDFNLK
                     NFIIHLAITVSRILDGYCLDNVLDVVLTDFQSNTTVENIFNYIESKYTIIISKADRVY
                     LYNHFITKSSLLDNVSNRVDTKIIEYVEEILEVINNQYTFDLRNDSVLFDDLVLHFKS
                     ILNSKSYNLNKVNPLINTIKSNYPLAFEITLNAIEKVFKNSIYSLTEDEIGYVSLHIG
                     AGIERFFQSNIKCKNVVLVCGSGYGSSRLLEVQLNKVFHDKINILQCLSFNQFLASEL
                     SDVDIIISTIPLNHDSIPIVLVDLKLLKKDIENISKSITNNSHIYSNLLDNFFDKNLF
                     IVNPKIKDKDELIKLMCNKLTQSEIVFPSFAESVFYRESLSSTNIDDFLAIPHPMELS
                     SIRTKICISILDEPIYWSEDSTVKLIMMLAINKDDYIKINSIYDILLKIIHDNDIRDS
                     ISNCNDFDNFLSIIKSIV"
     gene            2381..2833
                     /locus_tag="KR250_001765"
     CDS             2381..2833
                     /locus_tag="KR250_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBH0067701.1"
                     /translation="MVLQEKYIFIDKDLKSKEEVLNFIADSAYILGITTNKEALLKDL
                     LKRENEFPTGIKDGFAIPHTKSVNVKEASILYIKTTNELEWKTLDDSKVRYVFSLLAP
                     HKNEGNIHLKTLSKLATCLMEDDFTNEVKNASDASHLVKYITNKMKEE"
     gene            2836..3144
                     /locus_tag="KR250_001766"
     CDS             2836..3144
                     /locus_tag="KR250_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HBH0067702.1"
                     /translation="MKIVAITSCIAGLAHTPMAAKALEQAASKMGHEIKVEQQGAMGR
                     VNTITKEEAQDADFILIASDQTISGMERFDGKKVIKVKIGIALKKPEAVLTKCIEAIS
                     "
     gene            3258..4364
                     /locus_tag="KR250_001767"
     CDS             3258..4364
                     /locus_tag="KR250_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HBH0067703.1"
                     /translation="MSKKNKSLLSELKGHLMTGISYMLPLVIGSSLVVAIPKLTALAM
                     GITSLDAYKNVSGFYHILYLMEQVGWTGIGLLNTVLAGFIAFSIGEKPAMGAGFIGGL
                     IASNTNAGFLGAVIAGFFAGYVCKFLKKKIKISGSASGMMPLIIMPLITVGLTGFLMS
                     VVLAGPLGNINTSLNAWVAEMCQNGTNSVVLALILGAMIGFDLGGPVNKAAWMAGNAL
                     LLEGIYLPAILVNCAIVIPPLGYGLATFIRKSKFSNALAETGKGNIVMGIIGITEGAI
                     PFTLTNPLKLVPVNMIGCALGVGLSALLGVHAIMPPVGGLYGFISIGSGWAYLVGAIF
                     GALVIAILSTTLVDFNEDETTENTEFDEIELDIM"
     gene            4439..7117
                     /gene="mngB"
                     /locus_tag="KR250_001768"
     CDS             4439..7117
                     /gene="mngB"
                     /locus_tag="KR250_001768"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HBH0067704.1"
                     /translation="MKKTKVHVVPHSHWDREWYFTTSRSKIYLMKDFQDILDILEEKE
                     DFKYFTMDAQASLLDDYLKWRPQDEYRIRDLVQRRRLVIGPWYTQTDQLVISGESIIR
                     NLYYGIDRCNEFGEPMRVGYVPDSFGQSAQMPQIYRGFGIDSSLFWRGVSDDMVDTTE
                     FIWKGSDGSKVLAVQIPFGYYYGGNIPEDDSDLKEYLKDIIGKLKVKASTNNVYFPNG
                     FDQAPIRKNLPDILKKANEIDNENEYEISDIENYIDSVRNERNEFTELKGEFLNAKHM
                     RIHKSIFSSRSDLKIMNNKLENYITNVLEPLLTLSYSLGNEYPHLVIKDIWKLMFENA
                     AHDSIGSCNSDTTNEDVYTRYKQARDLSINLLDLHMRLISTQIKNNNEEITLTVFNGF
                     PEVRNEVVEFDTYIPGQDFVIKNKNGKILNYIIKEKEDITNYILTQTIRLNPSKKIYI
                     PKTVYRAKIALSVKDIPPMGYTQLVFELDTKNKCEIETSNCKEIENKYYKITANKNGS
                     LEILDKESNKTYKNQAVIEDNGDDGDSYNYSPPRKDIYVSSLDSVSSVEVLKSNIQEE
                     MILKYSLTIPTSLEERAIGKVSVKMPVTMKITLEPKEQIIKFNVNIKNNQALSHRVRV
                     LFNTEIASKFSIADQQFGIIQRPTVLEKDLALWKRDNYSWQEKPITIEPMQSFVTLED
                     GQRGIALITGCVREYQIVGDNLDTIALTLFRSFGYMGRENLLYRPGRASGEKIIATPD
                     AQLLKELNFDFGLYIYNNKFDEANVANTAKRFLTPIQIYEYADFLNGRLIFAFNDEKQ
                     IYENEFSLMNVNNSNFTVSAIKKEEKGDGIVVRIFNGMKNEDAKGSLNINKNVNDAYS
                     AMLDEIYDNTSKLKVNTKTVEVNSLSHCKVQTIVIK"
     gene            complement(7434..9767)
                     /locus_tag="KR250_001769"
     CDS             complement(7434..9767)
                     /locus_tag="KR250_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBH0067705.1"
                     /translation="MDTKLKKFKDKYIVTIIVFILMLSASLGMISQYSTIQASAKGGA
                     ENPFEQESFVDSIYKGSYVLYHNMKEEQEGKMIQPSKLFINEETMNYIKNVSKEKDTY
                     DGSETYYTDDSINADFNENFENWEHFIKASGKNLKYYLVDKESNFEFFNDNKELGVLD
                     TTKNDASKENSNENQSKNTEMNDEERQKQITDIKENYRFYVVMNFDKDGKVNIVDSYG
                     VNQKIINQMLLSKAREDLMDTSLGGNSISYKLSPIKNITLVYAVPKVISKGDSVEYGY
                     GNTYFDDGIISYISNVENNSYYKISGMILKIILAIVVIVALAFPYRKSKELLGFDTLS
                     RIPLEILFIALAIIQGLVDLYPTVIISNTLSGNYVEYLINNDISAKMASILVSLMNLT
                     YWFVIFSIIFVFIILLKHILNVGIREYFTKHTVTGMFFVWLKKAGKAVINQIRTINLK
                     EKPNKTIVTILGINLLIIVIMCSMWFFGIILALIYSVVLFKILSDYSKKTIYEYNQLL
                     DVTKKISDGNLDVNMEKDLGFFNPIKDELGNIQTGFKKAVDEEVKSQKMKTELISNVS
                     HDLKTPLTSIITYIDLLKDESITDENRKLYIDTLDRKSQRLQHLIEDLFEVSKANSGD
                     VYLNIVNVDIVSLMKQTLLELDDKLADSSLIVKNNFSNEKIILPLDSQRTFRVFENLI
                     INISKYAMPNSRVYIDILETDNKVNIMLKNMSASEIDFSVDDIMERFVRGDKSRNTEG
                     SGLGLAIAKSFIELQGGKMNISVDGDLFKVTITFNKN"
     gene            complement(9742..10458)
                     /locus_tag="KR250_001770"
     CDS             complement(9742..10458)
                     /locus_tag="KR250_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0067706.1"
                     /translation="MNSYNILVVEDEKEIADAIEIYLLNQGYNVFKGYNGLEGLKVIE
                     NQEIHLAIVDIMMPKMDGITLTMKLRENYNFPVIMLSAKSEEVDKIMGLNIGADDYVT
                     KPFKPLELLARVNSQLRRYTKYLNMVGNKEQKVESDDSVFAIGGLELNENTKEVSIDG
                     KYIKTTPIEFKILSLLMRNAGRVFSADEIYERVWNENAVNTDTVMVHVRNIREKIEID
                     PKNPKYLKVVWGVGYKIEKI"
     gene            complement(10873..11529)
                     /locus_tag="KR250_001771"
     CDS             complement(10873..11529)
                     /locus_tag="KR250_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBH0067707.1"
                     /translation="MELQDTIFKRQSVRKFKNQDVSDEDILKMIKAAGAAPSGKNIQN
                     WHFVVIKRRDLMEKIADVITKKQQEILVEMDKVSVDKANRFRKFVKNFTLFYLKAPVL
                     VLVFTKVYNPSGYYELELIDAPKETIDKLFIRNPGMQSLGAAIENFTLSAIELGYGSC
                     WLTSQNYAADEIEAVLEAETGFEKGEYFLGAMLALGVPEDNLKSPSKKPVEEICTFIK
                     "
     gene            complement(11584..12213)
                     /locus_tag="KR250_001772"
     CDS             complement(11584..12213)
                     /locus_tag="KR250_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBH0067708.1"
                     /translation="MVDSIIFDLDGTLWDSTEEVCKVWQDVLSEHKEIELSVTKDLFR
                     SLMGLSFEEIANRLFPNLSEEERMNLLNECSLKECDYLSENGAKLFDDIEDTLKLLSK
                     KYKLFIVSNCQAGYIESFLKAHKLEQYFIDFECPGNTGLHKGENNKLIIERNKLINPI
                     YVGDTQGDANSAKFVGIPFIYAKYGFGNVDVYDYFIDSFKDLLEHDILK"
     gene            complement(12344..12985)
                     /locus_tag="KR250_001773"
     CDS             complement(12344..12985)
                     /locus_tag="KR250_001773"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HBH0067709.1"
                     /translation="MKICIVLNGEIEDYEVTRDIIIKECYDCIICADGGANHTYKMEI
                     MPDYILGDLDSVEEEKINFYKNKGVKFEKFPSKKDETDTELCLFLAKTLKANHIDFFG
                     ALGGRIDHTLANIKLLYYLKEDGIYSRILSDKEEMYIVENEEISLYGNPGDTISVIAI
                     NGDAKGVTLTGLEYPLDNYYMKYSVPIGISNVMLSNSCKIKVEQGCVLVVRNL"
     gene            complement(12994..13665)
                     /locus_tag="KR250_001774"
     CDS             complement(12994..13665)
                     /locus_tag="KR250_001774"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HBH0067710.1"
                     /translation="MIKLAPSILSADFAKLLEDVRKVESAGCEYLHIDVMDGHFVPNI
                     TLGPLVVKSLKKENINMVFDAHLMIENPDQYIEEFVKAGCDIITVHQEACVHLHRTIQ
                     NIKSHGIKAGVVLNPATPVDTIKHVLSDLDMVLLMSVNPGFGGQSFIPCVLDKIKELK
                     AIIDSQGLNIDIEVDGGISPKNVAEVVQAGANVIVAGSAIFGSDDIQETVNLFRKNAS
                     LEELV"
     gene            complement(13670..14554)
                     /gene="rsgA"
                     /locus_tag="KR250_001775"
     CDS             complement(13670..14554)
                     /gene="rsgA"
                     /locus_tag="KR250_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HBH0067711.1"
                     /translation="MLEGKIIKGISGFYYVDTYNGIYECKARGIFRKQKITPLVGDRV
                     KISIVDEDEKKGILEEIDSRDTELIRPPIANVDKALIVFAIKNPKPNLSLLDRFIVLA
                     EKENLETVIILTKADLDDNDTLETVKNIYELSGYKVIPVSNITKLNIDKVKEELKENV
                     VVFAGPSGVGKSSLLNEIDENFKLQTGVVSDKIKRGKHTTRHAELLKLEFGGMVADTP
                     GFSSLALEDIEEVELKDYFIEFDKFNDCKFGSKCIHENEPNCAIKEAVTNGEISKERY
                     DSYIQLLHEIRQNNSRRY"
     gene            complement(14652..15389)
                     /locus_tag="KR250_001776"
     CDS             complement(14652..15389)
                     /locus_tag="KR250_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBH0067712.1"
                     /translation="MKKKTVLITGGARGIGKAMSKAFAKEGYNVLVNFNKSENEAKEL
                     YTILNEKNFSVKLFKANISNREDVEDMVDYCIKEFGGLDVLVNNAGVSQDKLFTDITD
                     EDWDNMMNINLKGSFYCSQVALKYMISEKKGNIINISSIWGISGASCEVHYSITKAGI
                     IGMTKALAKEVGPSNIRVNSIAPGVINTDMLSGYNEEDIDALVEETPLMRLGTPEDIA
                     NCAIFLASDKSNFITGQVISPNGGFVI"
     gene            complement(15497..17512)
                     /gene="pknB"
                     /locus_tag="KR250_001777"
     CDS             complement(15497..17512)
                     /gene="pknB"
                     /locus_tag="KR250_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HBH0067713.1"
                     /translation="MGDTILGNRYEIIRKIGDGGMAFVYEAKDRLLNRTVALKVLRPE
                     FVDDDEFLTKFKREAEAVASLSHPNIVNVYDVGEDGKVHYIVMEFVDGKNLKEIIQDE
                     GILDEYTALDITKQIAMALSAAHKKGIIHRDIKPHNILISNEGRVVKVADFGIAKAVS
                     NSTMTNIGSIIGSVHYFSPEQAKGKFVTNNADLYSLGIVLYEMLIGKVPFRGDSPISI
                     ALQHINDDIDFTSEEKVRIPQSVRTTIKKLTEKSSADRYQTAEELIEDIEYIEKNIDL
                     DFIKEYDDFATKKIDEKEINKVVNPTLAKPAPEKVVKPVEVADLDDDEDYYDDFYEED
                     DDEDEEEEEIMRAKKNQRPKSTPSKRTKKKKKKQESPKSRRRLKVIAAVLILILCAQV
                     FLAYKFLFAGGFGNKSLTVPNLVNMTLEEAQSAVEKEGLYLSVKSEEYNSEVDENCII
                     SQTPEGGSTNVKKGDTINVVVSKGSSQASVPNVVGLTLSNAKQLIEENNLKVGTVKYE
                     YSSIYKEGTVLSQSPGAGSSRAQEGDEVNLYVSKGSEKSNTQTPTVPDKKPTTPENNT
                     PTEPGSNSGNSGGNSGGSNSGNSGGNSGNNGSNSGGSNSGNSGGNSGGSNSGDNGGSS
                     GGSNSGDNGGSSGGSNSGDNGDSSGGSNSGDNGGTSSGTGANIGKTE"
     gene            complement(17529..18281)
                     /locus_tag="KR250_001778"
     CDS             complement(17529..18281)
                     /locus_tag="KR250_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HBH0067714.1"
                     /translation="MVYSCASHIGKIRKNNEDYCEGEVIDTEHGPIGIFAIADGMGGH
                     KKGEVASKLAVENIIDFLKENLLQHDNVKIDYIDDILKQAYNNVNSIVHKKSMEDIEF
                     EGMGTTLVTAIVYNNVLYVANVGDSRCYLLTDEKFDKITIDHSVVEELMMAHVITEEE
                     ARRHPQRNRITRAIGTDDMVVVDIFKKEIKKSDIILLATDGLTGFIDDEDIRDLILDY
                     EYERTSNISEELISMANDVSGKDNVSVIVIKV"
     gene            complement(18301..19332)
                     /gene="rlmN"
                     /locus_tag="KR250_001779"
     CDS             complement(18301..19332)
                     /gene="rlmN"
                     /locus_tag="KR250_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HBH0067715.1"
                     /translation="MEKKKIVLKNFTEDELKEFMKTIDEKPFRGSQIFSWIYKGAKTF
                     DDMNNIPKSLRNKLEEVSCIGHIDIELKLESKVDNTKKYLFLLDDGNIIETVMMDYDS
                     RVTVCVSNQVGCRMGCNFCASTMDGLIRNLEPWEILDQVIKIQEDTGKRVSNLVLMGS
                     GEPLDNFENTKQFLKIINEKNGLNIGYRHITLSTCGIVPKMYELADLEIAINLALSLH
                     SPYDEERRKIMPVANAYSIEEILNACRYYIKKTNRRVTFEYSLIKGVNDSEKEAKALA
                     KLLKGMLCHVNLIPINKVEEREYEKPDKAFIYKFRDSLEKNNIPATVRMSMGSDISGA
                     CGQLRRKYK"
     gene            complement(19340..20665)
                     /gene="rsmB"
                     /locus_tag="KR250_001780"
     CDS             complement(19340..20665)
                     /gene="rsmB"
                     /locus_tag="KR250_001780"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HBH0067716.1"
                     /translation="MDAREVGFKVLCDIEKNDNYSNIAINKHFKNLEISDMDRGLATE
                     LIYGVIENKYYLDYIINKLSKIKVKKMSTYVKIFLRMGTYQILFLDSISDYAAVNETV
                     KLSKKYDKKSSGFINAILRNEIRAKETIMDITEEDSVKYLSIKYSYNSWIIKNWIDKF
                     GQEFAEDLLEANNEKPSIYIRTNTLKISREELIEKLSEMGIVCLKVPMVEEAIKVEKM
                     KNIEKNELFKAGLFTIQDISSMIVGKVINPKEGSFVLDVCSAPGGKSTHLATLMNNTG
                     QVVARDIFEHKLKLINSTVNRLGLKNVFVERFDASEIDENSISKFDYVLADVPCSGFG
                     IIRRKPEIKYKKEEELKDITSIQKKILENASKYVKVGGTLVYSTCTIQDVENINIVTS
                     FVEENHNFEFVPIDTVNVDLDNQDKGYLKIYPNIHGIDGFFIAKLKRIR"
     gene            complement(20727..21428)
                     /locus_tag="KR250_001781"
     CDS             complement(20727..21428)
                     /locus_tag="KR250_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HBH0067717.1"
                     /translation="MYPVYGGFWGFDPTMVVLIPAILLTIYAQFKVSSTTNKYLRVNT
                     RRGYTGEQTARRVLDSNGLYDVKIEMVRGHLSDHYDPRRKAVRLSEDVYYGTSITSVA
                     VAAHECGHAIQHAKGYAPLQIRSSLVPVVNFASSISWFLIFLGFIMAGPFLKIGILLF
                     SASVLFQIITLPVEFNASSRAIVQLGNLGIIDESESRQSRRVLSAAALTYVAAALVSI
                     LQLLRLLLIAQRRND"
     gene            complement(21497..22249)
                     /locus_tag="KR250_001782"
     CDS             complement(21497..22249)
                     /locus_tag="KR250_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF116 domain-containing protein"
                     /protein_id="HBH0067718.1"
                     /translation="MTDIKKYLTSVGILILILGILIGVVNFFIISLTQIFSLMINIII
                     ISLILIILCSTVVTYRVIKGKYVNSNIMKINFNIVSGLFPIISFIASSFGMSKSDIRR
                     IYIKLNNEYIYSNKYNFNPEDIIILIPHCIQENSCKLKVTNDIDNCKECGRCNIGELI
                     KLKEKTNVKIFVATGGTLARKIIMDTKPKAVVAVACERDLTSGIQDIKKIPVLGVFNK
                     RPNGPCVNTNVDMMDIEKAIGFLTGKNILVCN"
     gene            complement(22259..23188)
                     /locus_tag="KR250_001783"
     CDS             complement(22259..23188)
                     /locus_tag="KR250_001783"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HBH0067719.1"
                     /translation="MKIVFMGTPDIAVPCLQKIIDEKYEILGVVTQPDKPKGRGKKLG
                     MSPVKELAIENNIPVYQPVKARDKEFIDKIKSLNPDVIVVVAFGQILPKEILEIPKLG
                     CINVHVSLLPKYRGAAPINWVIINGEEKTGVTTMYMDEGLDTGDMILKTEVNLDENIT
                     AGELHDKMMNIGAETLKETLRLIEEGNAPREVQNHEEFSYAPIMNKSLGNIDFSKSAR
                     EIHNLVRGVNPWPSAYTTYNDVIMKVWKTKVLDEKSTKDVGTIIDVSKDGIKVSTIDN
                     VLLIEEIQMPNKKRMLVGEYIKGNTIETGLVLG"
     gene            complement(23203..23643)
                     /gene="def"
                     /locus_tag="KR250_001784"
     CDS             complement(23203..23643)
                     /gene="def"
                     /locus_tag="KR250_001784"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HBH0067720.1"
                     /translation="MALRQIVQIGEPVLRKKSKKVEKIDEKIIQLLDDMAETMYDADG
                     VGLAAPQVGILKRVVVIDIGEELIELINPEIIETSGEQIDEEGCLSVVGEAGNVRRPN
                     YVKVRALNRNGETIELEGEELLARAFCHEIDHLDGILFVDKIEK"
     gene            complement(23661..26186)
                     /gene="priA"
                     /locus_tag="KR250_001785"
     CDS             complement(23661..26186)
                     /gene="priA"
                     /locus_tag="KR250_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HBH0067721.1"
                     /translation="MNNILNLSRGFIMKKYAKVIVRSNTIYTDNLFTYQIPVFLSDVI
                     KIGHRILVPFGKGNKPTEAFVFQFTDSLDEKIKIKEIIDILDENPIFRKEDLELVYWM
                     KNRYLCTYIECINLIYPKGYKLNNYKVVLLGESLSGLNDIELKEKISTLSDKNREIVE
                     KVIDSKGKIKVDKLKYIPNLNSSLYTMNKNGIIKLCWEYKNHKNEKKVCYISLSLESD
                     KIDDYIEQNKINVGSKQKEILSFLKNNENVEINDLLDLLNASKQSINSLSKKKLITLE
                     FKDYYREPKSIYKSVLKSIKLNNEQQEAVDEIKSNMFVDDKKPYLIHGVTGSGKTEVY
                     MEIIEFALNQGLDSIFLVPEIALTPQTIDRLKSRFGDLVGVFHSKLSEGEKHDVYKAV
                     KAGKVRVLIGARSALFAPFNSLGLIIIDECHESSYKSEKNPKFNAIEVARFMALKNNI
                     TLILGSATPSIEEYYRAKSGEYKLINIKSRANDKPLPNIEVVDMKDELDKGNRSIFSL
                     KLQKEIRYAIEENNQVILFLNRRGYANFVSCRKCGYVFQCENCDISLTYHKKSNTGRC
                     HYCGYEKEIPKECPECKSTYVKPFGVGTQKIEEELKYIFPDIKTLRMDKDTTSKKGAL
                     DEILNKFKDKEADVLIGTQMLSKGLDFENVTLVGILSADMILNFPDFKSFETTFQLIT
                     QVSGRAGRADKEGKVVLQTYDTEHYAIKHAIEYDYEGFYEDEIKIRKAFGYSPFNNML
                     SVVVSGEDERLVIKNIKNMHASLIYLLEKRGINDLGFILGPNPCSISKINQNYRWQIL
                     FKDENIEINLLKGIIKYICITKRDLIFDKNINVSIDINPNSVL"
     gene            complement(26425..27624)
                     /gene="coaBC"
                     /locus_tag="KR250_001786"
     CDS             complement(26425..27624)
                     /gene="coaBC"
                     /locus_tag="KR250_001786"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HBH0067722.1"
                     /translation="MLKDKTVVIGISGGIAAYKACDVVSKLKKLNANIHVIMTKSATE
                     FVKPLTFQSLSQNYVVEDMFEEPKTWEVEHISLAKKADLFLIVPASANVIGKIANGIA
                     DDMLTTTVMATKAQVLIAPAMNTNMYENPIVQKNIQTLKSFGYKFIEPESGRLACGDI
                     GTGKLANPDIIVEEVLTNLTKEQDLKGQSMIVTAGPTVESIDPVRYLTNRSSGKMGYS
                     IAREAIARGADVTLISGPTNLSIPQNLKKFVQIESAQDLYEAVVSNLSENKIVIQSAA
                     VADYKPKTYSDKKIKKKNDDLSIELSRNYDIAYEIGKIKEDKILIGFAAETNDLIEHA
                     KGKIKKKNLDFIVANDLTKEGAGFRADTNIVKIIDVEGNITEYPKMKKEEVANVILDK
                     VKALLEK"
     gene            complement(27629..27895)
                     /locus_tag="KR250_001787"
     CDS             complement(27629..27895)
                     /locus_tag="KR250_001787"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HBH0067723.1"
                     /translation="MLKPSINEVLEKIDNRYYLVGTVSKRARKLIDGEEPYVSNKTKE
                     KPVCVATKEVASGKITYRLLTEEEIEIEEARHHAEQHQQISEEE"
     gene            complement(27898..28515)
                     /gene="gmk"
                     /locus_tag="KR250_001788"
     CDS             complement(27898..28515)
                     /gene="gmk"
                     /locus_tag="KR250_001788"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HBH0067724.1"
                     /translation="MFVKKGLLLVVSGPSGAGKGTICKELLKENDTIKLSVSATTRKP
                     RTGEVDGVNYFFISKEKFEEMIEKGEFLEYAQIYDNFYGTPKAAIMECLEKGQDVLLE
                     IEMQGAKQIKEVCPEGVFIFVLPPSLEELKNRIVGRGTETEAEIEKRFSCAYEEIKMI
                     KDYDYFIFNEDVKTSAKEIEGIISSEKNKVSRYKNIIIEKFKEEL"
     gene            complement(28545..29426)
                     /locus_tag="KR250_001789"
     CDS             complement(28545..29426)
                     /locus_tag="KR250_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HBH0067725.1"
                     /translation="MAISMTGFGRGEYKDDNYYFLVECKTINHKYSDINIRLPRKISF
                     LEDKVRNLVKNYVKRGRVDLYIKFDLLGKEDVNLNFDEGLASQYIDILKEIKNKFDII
                     DDISVMNVAKFPDIVKIEEKEEDEDLLWSMLNQAVEDALIKLREMRSEEGKKLAEDIA
                     MRCDLLKNHIEEIEKYSSSVVEDYREKLNLRISELLDDPSIIDENRLAQEVAIYADKS
                     SITEEIVRFKSHIGQLKNTIFKDDSIGRKIDFLIQEMNRETNTIGSKSSDINITNLVV
                     EVKSELEKIREQIQNIE"
     gene            complement(29430..30260)
                     /locus_tag="KR250_001790"
     CDS             complement(29430..30260)
                     /locus_tag="KR250_001790"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HBH0067726.1"
                     /translation="MKFWKLHGIGNDFIAIDGRFDQINPSDYTALAKKVCHRRFSVGA
                     DGLLVVKDSKVADVEMVYYNSDGSRAAMCGNGLRCFCKFVYDNCIVKKESFTVDTLDG
                     IKDIKVNLTNREINSIRVNMGKGSFIAKDVPVLTNKERFVQESIKVLDRELKVTSMLM
                     GVPHTVVIVDELDVDDVCKYGKEIENNKIFPEKTNVNFVKVEDKYNIHVYTWERGCGY
                     TLGCGTGMTASAIVCNLLNMVESSVNVTSQGGTVKIDVGDFSYMTGPAVKICEGILEV
                     "
     gene            complement(30547..31581)
                     /gene="ltaE"
                     /locus_tag="KR250_001791"
     CDS             complement(30547..31581)
                     /gene="ltaE"
                     /locus_tag="KR250_001791"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HBH0067727.1"
                     /translation="MRFIDLRSDTVTMPTDEMREAMAKAPVGDSIFRDDPTVNKLEEL
                     AAAKVGKEDAIFLPSGTFGNQLALFTHCLRGQEIIIGKGYHIVTHEVGAPAVIAGVQL
                     RTIDEDESGALNPELVEKAIRRDDIHEPQTGLICVENAYSGGTVVSLDNLREIKKIAE
                     KHNLPVHLDGARLFNAALTLGVEAKEIAKCCDSVMFCVSKGLAAPMGSILAGNKEFIA
                     KARRKQKVMGGGMRQVGIVAAAGIIAIEKMTLRLNEDHENAKYLACELNKIDGIEVNN
                     VNPDISMVFFKMSEDIIKEDVLIKEFFERNIKINKMENGEYRFVTHVDITKEDLDYVL
                     KTLKELIGVC"
     gene            31877..33652
                     /locus_tag="KR250_001792"
     CDS             31877..33652
                     /locus_tag="KR250_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HBH0067728.1"
                     /translation="MALDGLVIHSIVDELSSKLTGGKIDKIHQPEDDEVIFNIRNNKE
                     NFRLVLSASASNPRVYLTSNYQKENPLKAPMFCMLLRKYIQGGNIVEISQIGFERIIK
                     ISVESLDELKEKTVKNIMIEIMGRHSNIIITHGEENKIIDSIKRVPFSISRVRQVLPG
                     HDYSLPPEQNKLNPLDDISKDLFIKNLEELEGPIFKSIYSKFLGISPIIAKEICYRAG
                     VNQNAIIKDISDEQFDALHKVFCNLFNDINSNKYSPCIIIDKKVDKVVDFSCINLTLF
                     SDLSYINKDSMSRILEDFYRTKDIKDRINQRSSDLKKSISVKLDRLYNKLKKQEEELS
                     ESENADIYKIKGELITSYIYMVEKGMESIEVANFYDENCNDVTIELNKNLTPSENAQK
                     YFKKYNKMKHAKVEISHQISLNKEEIDYLENIILSIENCENLAELQDIKEELAKVGYI
                     KTQKKNSKKDTIPSTKPHEFVSSDGFKILVGKNNKQNDYLTLRLADNDDLWMHTKNIP
                     GSHVIIKCAGKEVPDNTVFEGAMLAAFFSKSKLSSQVPVDYTKRKNVKKPSGSKPGMV
                     IYETNSTIYVTPEEETVAKLKVKSE"
     gene            complement(33867..35519)
                     /locus_tag="KR250_001793"
     CDS             complement(33867..35519)
                     /locus_tag="KR250_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBH0067729.1"
                     /translation="MLLVKVENLKKYYADKLILDIDKLEILENDKIGLVGSNGQGKTT
                     LLKAILGEIEIDEGYTYLTESYSYISQSENNIETCSHSKEKSLLNAPDKFEEHLSGGE
                     KVKLKIADALSNKKNIIIADEPTSNLDKKSIGVLEDMFKRHEGALLLISHDRRFLDEL
                     CTTILELEDGKLKAYKGNYTDYLMQKDEEVKRADFEYQEYVKEKKRLEKALLYKKALS
                     DGIRKTPKRMGNSEARLHKMGGQTNKKKLDSNVKAIKSRIDKLEVKNKPKVSKEMNIK
                     MQDGMEIISKNLVEVKDMTLKLENKLLLDNVSFKIKRGKKIALLGDNGCGKSTLIKEI
                     LADKNDNIKINNKVKVGYFDQNQSLLDEEKSVLYNTKVNSSFDESFIRINLSLFGFKG
                     DDVYKKVKVLSGGEKVKIALCKIILEDNNFLVFDEPTNYLDIKSMEALEKALINTDKT
                     MLIVSHDRVFVSHICNYIIEIKDAKIREFDCNYDEYTISRNKKTPSRENQIKKENLLV
                     LENRLTTVISMLSIEKDNLKKELYESEYNELLKQINKLKNSF"
     gene            complement(35818..37029)
                     /locus_tag="KR250_001794"
     CDS             complement(35818..37029)
                     /locus_tag="KR250_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HBH0067730.1"
                     /translation="MKKTIMSLQHLLAMFGATVLVPILTGFNPSVAIFCAGVGTLIFH
                     FCTEGKVPAFLGSSFAFIPVILAAKEAYGGDLAYAQGGIIVAGLIYIIMSIIVKVVGV
                     NKIKLYFPAQVTGAMIVVIGLNLLPTAFSMASANFVIAFITLAIAILTNKFGRGFIKQ
                     LGILIAVFSGYIICLILRLVDITTITEASLFAIPNFTVPKFSLGAIVIISPVVLAVFM
                     EHIGDMTTNGAVVGKNFIENPGLNRTLLGDGFATIVAGCLGGPANTTYGENTAVLAIT
                     KNYDPSILRRTAIFAILLACVGKFGGFLQSIPGSVMGGISIMLFSMITYVGLKTIRDS
                     SCVESKINILIIAVILLIGLGTTYLSNKGISVGIPITSTVKITGLSLAAIVGIVLNRI
                     LNNQDFKVEEE"
     gene            complement(37313..37846)
                     /gene="pyrR"
                     /locus_tag="KR250_001795"
     CDS             complement(37313..37846)
                     /gene="pyrR"
                     /locus_tag="KR250_001795"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001156491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HBH0067731.1"
                     /translation="MVEKAQLMDEKAIARAITRISHEIIERNKGVENLVLVGIKTRGV
                     PIANRISKKIEQIEGTKVDTGDIDITLYRDDLEKIHVEPVVKGTYLDFNVNDKTVVLV
                     DDVLYTGRTVRASLDAIIDIGRPKSIQLAVLVDRGHRELPIRADYVGKNVPTSRHEII
                     SVSLLEIDGEDSVTIKE"
     gene            complement(38206..39153)
                     /locus_tag="KR250_001796"
     CDS             complement(38206..39153)
                     /locus_tag="KR250_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBH0067732.1"
                     /translation="MFYFLKVKVGDIMDEVKEFLVLEEEEEVRLDIYLAEQLGDMSRS
                     YIQKIIKDGKVKVNNKVEKARYLVKEEDNIVIEIPEPKVLEVLPQDIPIDIVYEDDDV
                     LIINKPQDMVVHPAPGNYENTLVNGILYHCKDKLSSINGVIRPGIVHRIDKDTSGLLM
                     IAKNNYAHNFLAEQLKEHTITREYEFICYGVVKEDKITVDKPIGRNPKDRLKMAVVKD
                     GKNAITHFEVVERFDKFTHMRARLETGRTHQIRVHALSINHPLLGDYVYGPKETKFKL
                     KGQTLHAKKLGFIHPTTKEYIEFNSELPEYFKEVIKKIK"
     gene            complement(39122..39568)
                     /gene="lspA"
                     /locus_tag="KR250_001797"
     CDS             complement(39122..39568)
                     /gene="lspA"
                     /locus_tag="KR250_001797"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HBH0067733.1"
                     /translation="MLYILIIILLIGLDQLSKIWVLNNLVDVSTIPIINNVFHLTYVE
                     NRGAAFGLLQNNQWIFIIVALLATVFGLYYLNTRKVHIFGRLGIILIISGALGNLIDR
                     VRLGFVVDYFDFRIIWEYVFNIADVFVVVGTVFLCIYVLFFESKSR"
BASE COUNT        13179 a         6866 c         4293 g        15370 t
ORIGIN      
        1 ttcatgagac actctttttt gtatgaaatc agatatattt ttatcatttt atcaatgaaa
       61 aacaggtttc tctctctatt ttgagacaga atctttattg tataataatt ataatcaata
      121 caaaataata acaaattata caaaacttac ttatactatt tatgctaaaa acaaaaaata
      181 ttttattaaa gtttttaaaa cttatgtaat ataaaaatct atactataga cttgattatg
      241 gaaagaaaat tatcaaaatc attacaatta gatatactat ctctaatatc attgtcatga
      301 attatcttta aaaggatatc ataaatggag tttattttta tataatcatc tttatttata
      361 gccaacatca tgattaattt aacagtagaa tcttcactcc aatatattgg ttcatccaat
      421 atggaaatac aaatcttagt tcttatagag cttaattcca taggatgagg tatagctaaa
      481 aaatcatcta tattggtaga agataaactt tctctgtaaa atacagattc agcaaatgat
      541 ggaaatacaa tttcactttg agttaatttg ttgcacatca actttataag ctcatcttta
      601 tctttgattt taggattaac aataaataaa tttttatcaa agaaattgtc caaaagatta
      661 ctataaatat gggaattatt agtgattgac ttagatatat tctctatatc tttttttaat
      721 agttttaaat ccactaatac aataggaata ctatcatgat ttaaaggaat tgtcgatatt
      781 attatatcta catctgacaa ttcactagct aaaaattgat taaatgatag acattgaagt
      841 atatttattt tatcatgaaa aactttgttt aattgaacct ctaataacct acttgaacca
      901 taccctgaac cacatactaa tactacgttt ttacacttaa tattactttg aaaaaatctc
      961 tctattccag cacctatatg cagggataca tatccaattt catcttctgt taaagagtaa
     1021 atactatttt taaaaacttt ttctatagca tttaaagtta tttcaaatgc taaaggataa
     1081 ttactcttta ttgtatttat aaggggattc accttattta aattataaga tttagaattg
     1141 agaattgatt taaagtgcag tactaaatca tcaaataata cactatcatt tctaaggtca
     1201 aaagtatatt gattgttgat tacttctaaa atttcttcaa catactctat aattttagta
     1261 tctaccctat tggaaacgtt atccaaaaga gaacttttag ttataaaatg attatataga
     1321 taaactctgt ctgcctttga aataattata gtatatttac tctcaatata attaaatata
     1381 ttttcaacag ttgtattcga ttgaaaatct gttaaaacaa catctaaaac attatctaag
     1441 cagtaaccat caagtattct acttacagta atagctaaat ggataataaa gttttttaga
     1501 ttaaaatcta ggaaattgat attaaattta ttaatttcac ttaacactac cctttgaagc
     1561 tctattaaat ctacattatt aaataattct atttctctat ctgtaattcc aattatatag
     1621 ttttcaaaat tcctatttat catattatta gaaatacatc ttcgaatatc tatttcctta
     1681 ccttcaactt taactccata atatggctta gaaactaata ttaaattata ctttgacaaa
     1741 ataggcttta ttctttttat gtcatttagt attgtaaatt tactcacata caagctatta
     1801 gctaaatctt ctagttttat atatgtattg ttatagagaa tatattttaa aatatattga
     1861 tttctttcta taggagaatc tggtatttca cttgaatcca taatatcatc agatattaat
     1921 gctagatact gttggtataa tgctaaatta agaatatcta tataatatcc ttctcctttt
     1981 tttattttaa taatagcacc atttttctct aaagttccat ttatagaagt aatatcattt
     2041 cttatagttc tctcactcac ttttaaatga ttacttaaag ttttacaagt aattttatca
     2101 tcactttcat ttaataattt aaacaacgtg agcaatctct tgtgcataaa tatcccccct
     2161 tatagaattt cttctaaatt atactattta cactcatata atacaattac tctaataaaa
     2221 ttgtattata ataatttatt tccacttcta tatggaaatt agttattatc ataatataaa
     2281 tttccatgtc gaagtggaaa ttagttatat ttaatttaat taggataatg attacaatat
     2341 aaatataagt taagacatta tgaatggagt ggaaaaatat atggtactac aagaaaaata
     2401 tatatttata gataaagact taaaaagcaa agaagaagta ttaaatttta ttgcagatag
     2461 tgcttatata ctaggtatta caactaataa agaggctctt ttaaaagacc ttctaaaaag
     2521 agaaaatgaa tttccaacag gaataaaaga tggatttgcg atacctcata caaaaagtgt
     2581 aaatgtaaag gaagcttcca tactctatat taaaacaact aacgagttag agtggaaaac
     2641 tttagatgat agtaaggtta gatatgtatt tagtttgtta gccccacata aaaatgaagg
     2701 aaatatacat ttgaaaactc tatctaaatt agccacatgt ttaatggagg atgattttac
     2761 aaatgaagtt aaaaatgcta gtgatgcaag tcatttagta aaatatataa ctaataaaat
     2821 gaaggaggaa taattatgaa aatagtagca ataacatcat gtatagcagg attagcacac
     2881 acacctatgg cagcaaaagc tttagaacaa gctgcatcaa aaatgggtca tgaaatcaaa
     2941 gtagaacaac aaggtgctat gggacgagta aatactataa caaaagaaga agcacaagat
     3001 gcggacttta tacttatagc atctgaccaa actatatctg gaatggaaag atttgatggt
     3061 aaaaaggtga taaaagtcaa aataggtata gcgttaaaaa aaccagaagc tgtcttaaca
     3121 aagtgtatag aagctatttc ttaataaaaa aatctcactc taatatagaa tgagaaaaat
     3181 caaaaattaa aaaattgtct aatgtaatta taacatatta tcaaaaaata taacaaactt
     3241 atataggagg attaaaaatg agtaaaaaaa ataaatctct tttaagtgaa cttaagggac
     3301 acttaatgac aggtatctca tatatgttgc cacttgtaat aggctcatct ttagttgtag
     3361 ctatcccaaa gcttacagct ttagcaatgg gtataacaag tttagatgct tataaaaatg
     3421 tatctggttt ttatcatatt ctttacttaa tggaacaagt tggttggact ggcataggtc
     3481 tattaaatac ggtattagca ggatttatag cattttcaat aggtgagaaa ccagctatgg
     3541 gtgcaggttt tattggtggg ctcatagcaa gtaatactaa tgcaggattc ttaggagcag
     3601 taatagcagg tttctttgct gggtatgttt gtaagttttt aaagaaaaaa attaaaatat
     3661 ctgggtcagc atcaggaatg atgccactta taataatgcc acttataaca gttggattaa
     3721 ctggtttttt aatgagtgtt gtattagcag gacctttagg aaacataaat acttcattaa
     3781 atgcttgggt agctgaaatg tgtcaaaatg gaacaaattc ggtggtattg gctttaattt
     3841 taggcgcaat gataggattc gatttaggtg gaccagtaaa taaagcagct tggatggcag
     3901 gaaatgcatt attgttagag ggaatctatc taccagcgat attggtaaac tgcgcaatag
     3961 ttatacctcc actaggatat ggtttagcta cattcataag aaaatcaaag ttttccaatg
     4021 cattagctga aacaggaaaa ggcaatatag ttatgggtat aattggaata acagaaggtg
     4081 caattccttt cactttaaca aatccattaa aattagttcc tgttaatatg ataggatgcg
     4141 ctttgggtgt aggattatca gctttgttag gtgtacatgc tataatgcct ccagttggtg
     4201 gattatatgg atttatatca ataggttctg gatgggctta cttagtaggt gcaatatttg
     4261 gagctttagt aatagcaata ttatcaacaa ctttagtaga cttcaatgaa gatgaaacta
     4321 cggaaaatac agaatttgat gaaatagagt tagatataat gtaaattaaa aacttaattt
     4381 tagataattg agaattgagt gtactctcaa ttctcaatta aattttggga ggatttttat
     4441 gaaaaagaca aaagtacatg tagttccaca cagtcattgg gatagagaat ggtattttac
     4501 aacatcaagg tcaaaaatat atttaatgaa agattttcaa gatatattag acatattgga
     4561 agaaaaagaa gactttaaat actttactat ggatgcacaa gcttctctct tagatgatta
     4621 tttaaaatgg agacctcagg atgaatatag aataagagat ttagttcaaa gaagaagatt
     4681 agttattgga ccttggtata ctcaaacaga ccaattggtt atatcaggag aatctataat
     4741 tagaaacctt tactatggaa tagatagatg caatgaattt ggtgaaccta tgagggttgg
     4801 atatgtacca gattcttttg ggcaatcagc tcaaatgcca caaatataca gaggttttgg
     4861 aatagatagt tcattatttt ggcgtggagt ttctgatgat atggtagata caacagagtt
     4921 tatatggaaa ggttctgatg gttcaaaagt attagcagtt caaataccat ttggttatta
     4981 ctatggaggt aatataccag aagatgattc tgatttaaaa gaatatttaa aagatataat
     5041 cggaaaactt aaggttaaag cttctactaa taacgtatat tttccaaatg gatttgacca
     5101 agcaccaata agaaaaaacc ttcctgatat attaaaaaaa gctaatgaaa tagataatga
     5161 aaatgaatac gaaataagtg atatagaaaa ctatatagat tcagtaagaa atgaaagaaa
     5221 tgaatttaca gagcttaaag gtgaattttt aaatgctaaa catatgagaa tacacaaatc
     5281 catattctcc tcaaggtcag accttaaaat aatgaataac aaattagaaa attatattac
     5341 aaatgtactt gaaccattat taacattaag ttattcactt ggaaatgaat accctcacct
     5401 agttataaaa gatatatgga aacttatgtt tgaaaatgca gctcatgaca gcataggttc
     5461 ttgtaatagt gacacaacaa atgaggatgt atacacaaga tacaaacaag ccagagattt
     5521 gagtataaat ttattagatt tgcatatgag acttataagt actcaaatca aaaataataa
     5581 tgaagaaata acattaacag tattcaatgg attcccagaa gtcagaaatg aagttgtaga
     5641 atttgatact tatattccag gtcaagattt tgtgataaag aataaaaatg gaaaaattct
     5701 taattatata ataaaagaaa aagaagatat tacaaattat atattaactc aaacaataag
     5761 attaaaccca agtaaaaaaa tatatatacc caaaactgta tatagagcaa aaattgcact
     5821 ttcagtaaaa gatataccac caatgggata tactcaacta gtttttgaat tggatactaa
     5881 aaataaatgt gaaatagaaa caagtaattg caaggaaata gaaaacaaat actacaaaat
     5941 aacagccaat aaaaatggtt cattagaaat attagataaa gaatctaata agacttataa
     6001 aaaccaagct gtaattgaag acaatggaga tgatggagat tcatacaact attctcctcc
     6061 aagaaaagat atttatgttt catctttaga ttctgtatct tcagtagaag tgctaaaatc
     6121 aaacattcaa gaagaaatga tacttaaata ttcattgacc ataccaactt cactggaaga
     6181 aagggctata ggtaaagttt ctgtgaaaat gccagtaaca atgaaaatca ccttagaacc
     6241 taaagaacaa ataattaagt ttaatgttaa tataaaaaat aaccaagcac tatctcatag
     6301 agtaagagtt ttatttaata cagaaatagc atctaagttt tctatagcag atcaacaatt
     6361 tggaataata caaagaccaa cagtattaga aaaagacctt gcattgtgga aaagagacaa
     6421 ttactcatgg caagaaaaac ctataactat cgaaccaatg caaagttttg ttactttaga
     6481 ggatggacaa agaggaatag cattaataac tggttgtgta agagaatatc aaatagttgg
     6541 tgataattta gatacaatag cattaacact attcaggtca tttggatata tgggaagaga
     6601 aaatttactt tatagaccag gaagagcttc tggagaaaaa ataatagcaa caccagatgc
     6661 acaattactt aaagagttaa actttgactt tggtctttac atatataaca ataaatttga
     6721 tgaagcaaat gtagctaata cagctaagag attcttaaca cctatacaaa tatatgaata
     6781 tgctgatttt ttaaatggta gacttatatt tgcattcaat gacgaaaaac aaatatatga
     6841 aaatgaattt agtttgatga atgtaaataa ttcaaatttc acagtaagtg caataaagaa
     6901 agaagaaaaa ggagatggaa ttgtagttag aatcttcaat ggtatgaaaa atgaggatgc
     6961 taagggaagt cttaacatca ataaaaatgt caatgatgct tatagtgcaa tgctcgacga
     7021 aatctatgat aatactagca agcttaaagt aaatactaaa actgtagagg taaattcttt
     7081 atcacattgt aaagtacaaa caatagtaat taaataatat aaaatagctt atacaagatt
     7141 tcaaaataaa ttgctagtac tatgttaata gtaaagccag atattatgct atttataagc
     7201 tctgtaacac taaataacat tttgtaaaat aaaaaggtga accttgtata ttaggattca
     7261 ccttttttaa ataaatcaac attctgtttt aaaataacat ttataagctg ttttgaaata
     7321 atgcttacaa attattttaa aacaatacct ataaattttt atttaaagaa tacacataaa
     7381 tatttccaga ttcttttatt ctatagactt tataattatt aaagaagtct aatctaattt
     7441 ttgttaaaag taattgtaac tttgaataaa tcaccatcca cacttatatt catcttacca
     7501 ccctgtaatt ctataaaact ttttgctatc gctaaaccaa gaccagaacc ttctgtgtta
     7561 cgtgatttat ctcctcttac aaatctctcc attatatcat ctacactaaa atcaatctca
     7621 gatgcagaca tatttttaag cataatattt actttattat ctgtttctaa aatatcaata
     7681 tacactcttg aatttggcat tgcatatttg cttatattta ttataagatt ttcaaatact
     7741 ctaaatgtac gctgactatc taatggtaat attatttttt cattagaaaa gttattcttt
     7801 actattagag atgaatctgc aagtttgtca tctaattcta aaagtgtctg cttcattaga
     7861 gatacaatat ccacatttac tatattcaaa taaacatctc cactatttgc cttgcttact
     7921 tcaaacaaat cttctattag atgttgcaat ctttgtgact ttctgtctaa tgtatcaata
     7981 tacaacttac gattttcatc tgttatactt tcatctttta ataaatctat ataagtgatt
     8041 atagatgtta gaggtgtttt taagtcatgt gatacatttg atataagctc tgttttcatc
     8101 ttttgactct taacctcttc gtcaacagct tttttaaatc cagtttgaat atttcctaat
     8161 tcatctttta taggattaaa aaatcctaaa tctttttcca tatttacatc taagtttcca
     8221 tctgaaattt tctttgtaac atctaaaagt tgattatatt catatattgt ctttttagag
     8281 taatctgaca gtatcttaaa taatactact gaatatatta atgcgagtat tattccaaag
     8341 aaccacatac tacacattat aactataatc aataaattta ttcccaaaat tgtaactata
     8401 gttttatttg gcttttcttt cagatttatt gttctaattt gattaataac tgcttttcct
     8461 gcttttttta accatacaaa aaacattcct gtcacagtat gttttgtaaa atactctctt
     8521 attccaacat ttaaaatgtg ttttaaaagt attataaata caaatattat tgagaatatt
     8581 acaaaccaat aagttaaatt cattaaactt accaatatac tagccatttt agcacttatg
     8641 tcattgttta taaggtactc tacataattc ccactcaagg tattacttat tataacagta
     8701 ggatataaat ctacaagccc ctgtataata gcaagagcaa taaagagtat ctctaacggt
     8761 attcttgata atgtatcaaa tcctagcaac tctttagatt ttctatatgg gaatgctaaa
     8821 gctacaataa caactatagc taatataatt tttaaaatca ttccagaaat cttataataa
     8881 gaattatttt ctacattgct tatatatgaa attataccat catcaaaata agtatttcca
     8941 taaccatact ctacactatc acctttactt attactttag gtacagcata tactaatgtt
     9001 atatttttta ttggagataa cttatatgaa attgaatttc ctcctaaact tgtatccatt
     9061 aaatcttctc ttgctttaga taacaacatt tgattaataa ttttttgatt tactccataa
     9121 gaatccacaa tatttacttt tccatcttta tcaaaattca ttacaacgta gaatctatag
     9181 ttttctttta tatctgtaat ctgcttttgt ctttcctcat cattcatttc tgtatttttt
     9241 gactgatttt cattagaatt ttcttttgaa gcatcatttt ttgttgtatc aagtactcct
     9301 aattctttat tatcattaaa aaattcaaaa ttgctttctt tatcaactaa atagtacttt
     9361 aaattctttc ctgacgcttt aatgaaatgc tcccaatttt caaagttttc attaaaatct
     9421 gcattaatac tatcatctgt ataataagtt tctgaaccat cataagtatc tttttcttta
     9481 cttacatttt ttatatagtt catagtttct tcattgataa atagctttga tggttgtatc
     9541 atcttacctt cttgttcttc cttcatatta tgatataaaa cataacttcc tttatatata
     9601 ctatctacaa agctttcctg ttcaaatgga ttctctgctc cacctttagc acttgcttgt
     9661 atagtagaat attgactaat catacctaat gatgctgata acataagtat aaaaactata
     9721 atagtaacta tatatttatc tttaaatttt ttcaattttg tatccaacac cccacaccac
     9781 ctttaaatat ttaggatttt ttgggtcaat ttctatcttt tcccttatat tcctcacatg
     9841 taccataact gtatctgtat ttacagcatt ctcattccaa actctttcat atatttcatc
     9901 agcagaaaaa actcttcctg catttctcat aagtaaactt agtattttaa attctatagg
     9961 agttgttttt atatactttc catcaatact aacttctttt gtattttcat ttaattctaa
    10021 tccgcctatt gcaaacacac tatcgtcact ttctaccttt tgctctttat ttccaaccat
    10081 gtttagatac ttagtgtacc ttctaagttg tgaattaact ctagccaata gttctaatgg
    10141 cttaaaaggt tttgttacat aatcgtctgc tcctatatta agccccatta ttttgtcaac
    10201 ttcttctgat tttgcagata gcattattac tggaaaatta tagttttctc taagcttcat
    10261 tgtaagtgtt ataccatcca ttttaggcat cattatatca actatagcta aatgtatttc
    10321 ttggttttct ataaccttaa gaccttctaa tccattgtat cccttaaaaa cattatatcc
    10381 ttgatttaat agatatattt ctatagcatc tgctatctcc ttttcatctt ctaccaccaa
    10441 aatattatat gaattcattc taatctcctt tatattaatt tataaatttt ctttacatat
    10501 atgattatac aatatgatag aagtttttcc atttatatat atatttatat cttttgttct
    10561 ggttatttgt atttaatgtc catcttagaa acctccttct agtttaaatt taatattttt
    10621 tcattttgtt ttatatactt aatattaaaa ttaaaaactt aagatattac atatactttt
    10681 ctaaagattt tctaaagatt taaatatatt taaaattaat atacaaaaaa aagtagttct
    10741 tattgtatta attaaattaa tgatttgaaa tataaaatta atatcaataa aacctacttt
    10801 ttaatttttt aaatatttat ttttaattta ttttgttttt aatttacttc gattttaaaa
    10861 tgtccttgac ttttatttta taaatgtaca tatttcttct actggttttt tagatggact
    10921 cttcaaattg tcctctggca ctccaagagc taacattgca cctaaaaaat actctccctt
    10981 ttcaaaacca gtttctgctt ctaaaacagc ttctatctca tctgctgcat agttttgact
    11041 tgtcaaccaa caagaaccat atccaagttc aattgctgat agtgtaaagt tttcaattgc
    11101 agcacctaga ctctgcatac ctggatttct tataaataat ttatctatag tctccttagg
    11161 tgcatctata agctctaatt cataatatcc tgatggatta tatacctttg taaaaactaa
    11221 tactaaaact ggagctttta agtaaaatag tgtaaaattt ttaacaaatt ttctaaatct
    11281 attagcctta tccactgata ctttatccat ttctacaagt atttcttgtt gctttttagt
    11341 tatcacatct gctatctttt ccattaaatc acggcgtttt ataacaacaa aatgccagtt
    11401 ttgaatattc ttgcctgatg gtgctgcacc tgctgctttt atcattttta aaatatcttc
    11461 atctgataca tcttgatttt taaactttct tacactttgt cttttaaaaa tagtatcttg
    11521 taattccatt tttaaatccc cccataactt ttttattatt ttaatataat ttttaaactc
    11581 atcttatttt aatatatcat gttctaacaa atctttaaat gaatctataa aataatcata
    11641 tacatctaca ttcccaaacc catatttggc atatataaaa ggtataccta caaacttagc
    11701 actatttgca tcaccttgcg tatcaccaac atagattgga tttattaatt tatttctttc
    11761 aattataagt ttattatttt ctcctttatg caaaccagta ttaccaggac attcaaaatc
    11821 tataaaatac tgttctagtt tatgtgcttt taaaaaagat tctatatatc cagcttgaca
    11881 attacttact ataaataatt tatatttttt agatagtaat ttaagagtat cttctatatc
    11941 atcaaataat ttagctccat tttcagataa atagtcacat tctttcaaag aacattcatt
    12001 caataaattc attctttctt cttcacttaa atttggaaat aaacgatttg ctatttcctc
    12061 aaaagaaagc cccataagac ttctaaataa atcttttgtg acacttaatt ctatttcctt
    12121 atgctcactt aaaacatcct gccagacttt gcatacttct tcagttgaat cccacaatgt
    12181 tccatctaaa tcaaaaataa tactatctac cacaaaaaat cccccttatg tcaaagttta
    12241 ttatatattt ataattatat actaaaaagc agatacaatc ataaatatat tttacttgat
    12301 tgtatctgct ctacaatata aaaccaattg tcaaaatcaa attttataaa tttctaacta
    12361 caagtacaca gccttgctct actttaatct tacaagaatt actcaacata acatttgata
    12421 ttccaatagg tacagagtat ttcatataat aattatcgag agggtattct aagccagtaa
    12481 gtgttacacc cttagcatca ccatttatag ctattacaga aatagtgtcc cctgggtttc
    12541 catacaatga aatttcctca ttttcaacta tatacatctc ttctttatca gaaagtatcc
    12601 tagaatatat accatcttct tttaaataat ataatagctt tatatttgca agagtgtggt
    12661 caatccttcc ccctaaagca ccaaaaaaat caatatgatt tgctttaagt gtcttagcta
    12721 aaaaaagaca aagctctgta tcagtctcat cttttttaga tggaaatttt tcaaatttaa
    12781 cacctttatt tttatagaag tttatctttt cttcttctac tgaatctaaa tctcctaata
    12841 tataatctgg cattatttcc attttataag tgtgatttgc ccctccatct gcacatatta
    12901 tgcagtcata acattcctta attataatat ctcttgtaac ttcgtaatct tcaatttcgc
    12961 catttaaaac aatacagatt ttcattatta aatttaaact aattcttcaa gagaagcatt
    13021 ctttctaaat aaattaactg tttcttgaat gtcatcactt ccaaatattg cagaacctgc
    13081 tactattaca ttagctcctg cttgtacaac ttcggctacg tttttagggc ttataccacc
    13141 atcaacttct atatcaatgt ttaatccttg actatctata atagctttta actctttaat
    13201 cttatctaaa acacaaggaa taaatgattg tcctccaaat ccagggttaa cagacattaa
    13261 taataccata tctaaatcag atagtacatg tttaatcgta tctactggtg tagctggatt
    13321 aagtactaca ccagctttta ttccatgtga ttttatattt tgtatagttc tatgtagatg
    13381 aacacaagcc tcctggtgaa cagttattat atcacatcct gcttttacaa attcttcaat
    13441 atactggtct ggattttcta tcataagatg agcatcaaat accatattta tattttcttt
    13501 ctttaaactt ttgactacaa gtggtcctaa tgtaatgttt ggtacaaagt gtccatccat
    13561 tacatcgata tgaagatatt cacatcctgc actttctact tttcttacat cttctaataa
    13621 ttttgcaaaa tctgctgata atattgatgg tgctaattta atcatgtttc taatacctcc
    13681 tactattatt ttgtcttatt tcatgtagca gctgtatata actatcatat ctttctttag
    13741 atatttctcc atttgtaaca gcttctttta tagcacaatt aggttcattt tcatgaatac
    13801 actttgaacc aaacttacaa tcattaaatt tatcaaattc tataaaataa tctttaagtt
    13861 caacttcctc tatatcttca agtgcaaggg aactaaaacc tggtgtatct gcaaccattc
    13921 caccaaattc taattttaaa agttcagcgt gacgagtagt atgttttccc ctttttattt
    13981 tatcacttac aactcctgtt tgaagtttaa aattttcatc tatttcattt aataagcttg
    14041 attttccaac tccagatggt cctgcaaaca ctactacatt ttcttttaat tcttctttta
    14101 ctttatctat atttaactta gtaatattgc ttacaggaat gactttataa ccactaagtt
    14161 catatatatt tttaacagtt tctaatgtat cattatcatc taaatctgct tttgtaagga
    14221 ttattacagt ttctaaattt tctttttctg ccaaaactat aaatctatct aaaagagata
    14281 agtttggttt aggatttttt attgcgaata ctattaatgc tttatctaca tttgcaatag
    14341 gaggtcttat aagttctgta tctctactgt cgatttcttc taagataccc tttttttcat
    14401 cttcatcaac aatgcttatt ttgactctat caccaaccaa aggtgttatt ttctgctttc
    14461 taaatatccc tctagcctta cattcataaa taccattata agtatctaca taataaaatc
    14521 cactaatccc ctttataatt tttccttcta gcatcaaatg cctcccttta taaaatttat
    14581 ataaacacat actgtttatg agtagtatat cgtgttattc tttcctattt ttatataata
    14641 ccacattttt tttaaataac aaatcctccg tttggactaa ttacctgacc agtgataaaa
    14701 tttgatttgt ctgatgccaa gaaaatagca caatttgcta tatcttctgg agttcctagc
    14761 ctcattagag gggtttcttc cacaagagcg tctatatctt cctcattata tccagataac
    14821 atgtcagtat taatgacacc tggagctatg ctatttactc taatatttga tggtcctact
    14881 tctttagcta atgcctttgt catacctatt atacctgctt ttgttataga ataatgcact
    14941 tcacaagacg cacctgatat accccagatt gaagatatat taattatatt tccttttttt
    15001 tctgatatca tgtattttaa agcaacttgt gaacaataaa agcttccttt taaattaata
    15061 ttcatcatat tatcccaatc ttcatcagtt atatctgtaa acaacttatc ttgacttaca
    15121 ccagcattgt ttacaagtac atccaatcct ccaaattctt ttatacaata atctaccata
    15181 tcctctacat cttctctatt tgaaatatta gccttaaaaa gttttacaga aaaatttttt
    15241 tcatttaata tagtgtacaa ctctttagct tcattttctg acttattaaa atttactaat
    15301 acattgtaac cttcttttgc aaaagcttta gacatagctt taccaatccc tctagctcct
    15361 cctgtaatta aaactgtttt cttcttcata aatacttttc tccttatatt ataaccaatt
    15421 cagtatttta tacaaaacaa actgcccttt aaagaaaata ttttctataa aaggacagct
    15481 cttattttat taatatttat tctgttttac caatgtttgc gccagttcct gatgaagttc
    15541 ctccattatc tcctgagttt gagcctcctg aactatctcc attgtctcct gagtttgaac
    15601 ctcctgaact tcctccattg tctcctgagt ttgagcctcc tgaacttcct ccattatctc
    15661 ctgagtttga gcctcctgag tttccaccac tatttcctga gtttgaacct cctgagttac
    15721 ttccattatt gcctgagttt ccaccactat ttcctgaatt tgagcctcct gaatttccac
    15781 cactgtttcc tgagtttgaa ccaggttctg ttggtgtatt attctctggt gttgttggtt
    15841 ttttatctgg tactgttgga gtctgtgtat ttgatttttc tgaacctttg cttacatata
    15901 gatttacctc atcaccttcc tgagctcttg atgagccagc acctggactc tgacttaaaa
    15961 ctgtaccttc tttatatatt gagctgtatt catacttaac agttcccact tttaagttat
    16021 tttcttcaat aagttgtttt gcattactca aagtaagtcc cactacatta ggaacagatg
    16081 cctgacttga acctttactt acgacaacat ttatagtatc accctttttg acatttgtac
    16141 ttccaccttc aggtgtctgt gatattatac aattttcatc aacttcacta ttatactctt
    16201 cactttttac actcaggtat aatccttctt tttcaacagc actttgagct tcttctaatg
    16261 tcatatttac aagattagga acagtcaaag atttattgcc aaaccctcca gcaaataaaa
    16321 acttatatgc taagaatact tgagcacaca atatcagtat caaaacagcc gcaattactt
    16381 ttaatcttct tctagatttt gggctttcct gttttttctt ctttttctta gttctcttac
    16441 ttggagtact cttaggtctc tgattctttt tagctctcat tatctcttcc tcttcttcat
    16501 cttcatcatc atcttcttca taaaaatcat catagtaatc ttcatcatca tccaaatcag
    16561 ctacttcaac aggcttaacc actttttcag gagctggttt tgcaagtgtt ggatttacaa
    16621 ctttattaat ttctttttca tcaattttct tagttgcaaa atcatcatat tcttttataa
    16681 aatctaaatc aatatttttt tctatatact ctatatcttc tatcaattct tcagcagttt
    16741 gatatctatc tgcactagat ttttctgtaa gcttttttat tgtagttctt acactttgag
    16801 gaatcctaac tttttcttct gatgtaaagt ctatatcatc attaatgtgt tgaagtgcta
    16861 tagaaattgg actatctccc ctaaatggaa cttttcctat caacatttca tataaaacta
    16921 tacctagaga atacaggtca gcattattag ttacaaattt acctttagct tgctctggtg
    16981 aaaaataatg cactgaacct attatactac caatattagt cattgttgaa tttgaaacag
    17041 ctttggctat accaaaatca gccactttta ctactcttcc ttcatttgat atgagaatat
    17101 tatgaggttt tatatctcta tgtataatcc cctttttgtg cgctgcacta agtgccatag
    17161 ctatttgttt tgttatatct aatgcagtat attcatctaa tataccttca tcttgtataa
    17221 tctcttttag attcttccca tctacaaact ccattacaat gtagtgaact tttccatctt
    17281 caccaacatc ataaacattt actatatttg gatgtgaaag acttgctact gcttctgcct
    17341 ctcttttaaa ctttgttaga aattcatcat catctacaaa ttcaggtcta agtactttaa
    17401 gtgcaacagt cctgtttaat aatctgtctt tagcctcata tacaaatgcc attcctccat
    17461 caccaatctt cctgatgatt tcatatcgat ttcctaaaat tgtatctccc acaatatcac
    17521 cgtcctaatt atacttttat cactataacc gaaacattat ctttaccaga aacatcattg
    17581 gccatactta tcaattcttc acttatatta ctagttctct catattcata atctaatatt
    17641 aaatctctta tatcttcatc atcaataaac ccagttaatc catctgtcgc aagaagtatt
    17701 atatcacttt ttttgatttc tttcttaaaa atatctacaa caaccatatc atctgttcca
    17761 attgctctag ttattctatt cctctgtgga tgtcttcttg cctcttcttc tgtaatgaca
    17821 tgggccatca taagttcttc aaccacggaa tggtctatag ttattttatc aaatttttca
    17881 tctgttaaca aataacatct gctatctcca acatttgcca catataagac attattatac
    17941 actatagccg taactaatgt agttcccatt ccttcaaatt ctatatcttc catagatttt
    18001 ttgtgcacaa ttgagtttac attattataa gcctgtttta atatatcatc tatatagtct
    18061 atttttacat tatcatgttg taataagttt tcctttagaa aatcaattat attttctact
    18121 gctaatttac tggcgacttc acctttttta tgtccaccca ttccatcagc tatggcaaat
    18181 attcctattg gaccatgttc tgtatctata acttctccct cacaatagtc ttcattgttt
    18241 tttcttattt ttcctatatg ggaggcacaa ctataaacca tacgacttcc tccttacaaa
    18301 ttacttgtat tttcttctca attgcccaca agctccacta atatcagaac ccatagacat
    18361 cctaactgtg gcaggtatat tgttcttctc caaactatcc ctaaatttat aaataaatgc
    18421 tttatctggc ttttcatatt ctctttcttc aactttattt attggaatta aatttacatg
    18481 acatagcata ccttttagga gcttagctaa tgcctttgct tctttttctg agtcatttac
    18541 cccttttata agagaatatt caaatgtaac ccttctattt gtctttttta tataatatct
    18601 acatgcattc aatatttctt caatagaata agcatttgct acaggcataa tttttcttcg
    18661 ttcttcatca tatggtgaat gaagtgataa agctaaattt atagctattt ctaagtctgc
    18721 caactcatac attttaggta ctattccaca agttgaaaga gttatatgcc tataccctat
    18781 attaagacca tttttttcgt taattatttt taaaaattgt tttgtatttt caaagttatc
    18841 aagtggctca ccactcccca tcaatacaag atttgatacc ctcttaccag tatcttcttg
    18901 aattttaata acttggtcta aaatttccca tggctctaag tttctaatta atccatccat
    18961 tgtagatgca caaaaattac atcccatcct acaacctact tggttagaaa cacagacagt
    19021 aactctacta tcataatcca tcatcacagt ttctattata tttccatcat ctaataaaaa
    19081 caaatatttt tttgtattat ctaccttaga ttctaacttt aattctatat ctatatgccc
    19141 tatacatgaa acctcttcta atttatttct caaacttttt ggtatattgt tcatatcatc
    19201 aaaagttttt gcacccttat atatccaaga gaatatttga cttcctctaa agggcttttc
    19261 atctatagtt ttcataaatt ctttaagttc atcttcagta aaatttttta atactatctt
    19321 tttcttttcc attctctcac taccttattc tctttaattt tgctatgaaa aaaccatcta
    19381 taccatgaat attaggatat atctttaaat atcctttatc ttgattgtct aagtctacat
    19441 ttactgtatc aattggaaca aattcaaagt tgtgattttc ttcaacaaaa gaagtaacta
    19501 tatttatatt ctccacatct tgaattgtac atgtactata tactaaagtt ccaccaactt
    19561 tgacatattt agatgcattt tctaaaattt tcttttgtat agaagtgatg tcttttaact
    19621 cttcttcttt cttatactta atttctggtt tacgtcttat tataccaaaa ccagagcaag
    19681 gaacatctgc taaaacataa tcaaatttac ttatactatt ttcatcaatc tcagaagcat
    19741 caaatctttc tacaaacaca tttttaagcc ccaatctatt tacagtagag tttataagct
    19801 ttagtttatg ctcaaaaata tctctagcta ctacttgacc agtattgttc attaaagtag
    19861 ctaagtgagt agatttccct ccaggagcac tacatacatc caaaacaaat gagccttctt
    19921 ttggattaat tactttccct actatcatag aacttatatc ttgaattgta aataaaccag
    19981 ccttaaataa ttcatttttt tctatatttt tcattttttc gactttaata gcttcttcta
    20041 ccataggaac ttttaaacat acaataccca tttcacttaa tttttcaata agttcttctc
    20101 tacttatttt aagtgtattc gttcttatat agatactagg cttttcatta tttgcttcca
    20161 acaaatcttc agcaaattct tgtccaaatt tatctatcca gttttttata atccaagagt
    20221 tataggaata ctttatagat aagtatttta cactatcttc ctcagttatg tccattatag
    20281 tctctttagc tcttatttca tttctaagaa tagcatttat gaaaccagaa gattttttat
    20341 catatttctt agataatttt acagtttcat taactgctgc ataatctgat atactatcta
    20401 aaaataaaat ctgatatgtt cccattctta aaaagatttt aacataagtt gacatttttt
    20461 ttacctttat ttttgaaagt ttattgatta tataatctaa ataatattta ttttctatta
    20521 ctccataaat taactctgtt gcaagtcctc tatccatatc acttatttct aaatttttaa
    20581 aatgcttatt tattgcaata tttgaataat tgtcattttt ttctatatca cataatactt
    20641 taaatccaac ttctcttgca tccatataaa tttctccctt ttattaaaca taaaaaaggg
    20701 ttgtttctaa acaacccact ttacttttag tcatttcttc tttgagctat tagtaacaat
    20761 ctaagtaatt gaagtattga aaccaatgct gctgcaacat atgttaaagc tgctgctgaa
    20821 agtactcttc tactttgtct tgactcactt tcatctataa tacctaaatt cccaagttga
    20881 acgattgctc tacttgaagc attaaattct acaggcaatg ttattatttg gaataacact
    20941 gaagccgaaa acaatagtat accaattttt aaaaatggac cagccataat aaatcctaaa
    21001 aatattaaaa accaagatat acttgaagca aaattcacta ctggtactaa actacttctt
    21061 atttgaagag gtgcataacc tttagcatgt tgtatggcat gaccacattc atgtgcagct
    21121 actgcaacag aagttataga tgttccataa tatacatctt ctgacaacct tacagccttt
    21181 cttcttgggt cataatggtc acttagatgc cctctaacca tttctatctt tacatcatac
    21241 aaaccatttg aatcaagtac tcttcttgct gtttgctctc cagtatatcc tcgacgtgta
    21301 tttactctta aatacttatt tgttgttgaa ctcactttaa attgtgcata tatagtaagt
    21361 aatatagctg gaattaatac aaccattgtt gggtcaaatc cccaaaaacc gccatataca
    21421 gggtacataa ataacactct cctaaaatat atttttatta tacatcctct taatattcac
    21481 ttaacttaaa aacagtttaa ttacaaacta atatattttt tcctgttaaa aagcctatag
    21541 ctttttcaat atccatcatg tctacattag tatttacaca aggtccatta ggtcttttgt
    21601 taaatacacc taaaacagga attttcttaa tatcttgtat tccagatgtc aaatctcttt
    21661 cacatgcaac agctacaaca gctttaggtt tagtgtccat tataattttt ctagctagag
    21721 ttcctcctgt agctacaaat atttttacat tagttttttc ctttagctta attaattctc
    21781 ctatgttgca tcttccacat tctttacaat tatctatatc attagtaact tttaatttac
    21841 aactattctc ttgaatacaa tgaggtatta aaattattat atcctctgga ttaaaattat
    21901 atttattact gtatatatat tcattattta actttatata tattcttcta atatcacttt
    21961 ttgacatacc aaatgatgat gcaatgaaag atataatagg aaataatcca ctgacaatat
    22021 taaaatttat tttcattata tttgagttaa catactttcc tttaattact ctataagtaa
    22081 ctactgttga acatagtatt ataagtatta gtgatattat tataatattt atcatcaagg
    22141 agaatatttg agttaaactt attataaaaa aattaacaac tcctatcaaa attcctaata
    22201 ttaaaatcaa aatcccaaca cttgttaaat acttctttat atctgtcata cttttcacct
    22261 aacccaatac taaaccagtt tctatagtat tcccttttat atattcacca accaacattc
    22321 tctttttatt cggcatctga atttcttcaa ttaaaagtac attatctata gtacttactt
    22381 ttatcccatc tttgcttaca tcaattattg tacctacatc ttttgtgctt ttttcatcta
    22441 acactttagt tttccaaact ttcattatga catcattata ggtagtataa gcacttggcc
    22501 atggatttac tcctctaact aaattgtgta tttctctggc actttttgaa aaatctatgt
    22561 tacctaatga tttattcatt attggcgcat aggaaaattc ttcatgattt tgtacttctc
    22621 ttggagcatt tccttcttca attaatctta atgtttcttt taaagtttct gctcctatat
    22681 tcatcatttt atcatgaagt tctcctgctg ttatattttc atcaagattt acttctgtct
    22741 ttagtatcat atctccagta tctaatcctt catccatata catagttgta acaccagtct
    22801 tttcttcacc atttattatt acccaattta taggtgccgc acctctatat tttggaagta
    22861 aagaaacatg aacatttata catccaagtt taggaatctc taatatttct tttggaagta
    22921 tctgtccaaa agctacaact actattacat ctggatttaa agatttaatt ttatctataa
    22981 attctttatc tctagccttt actggttgat atacaggtat attattttca attgccagtt
    23041 cttttactgg actcatacct agttttttac ctcttccttt tggtttatct ggttgagtta
    23101 caacacccaa tatttcatac ttctcatcta ttattttttg taaacatgga actgctatat
    23161 caggagttcc cataaatact attttcatca aatcaactcc tactactttt ctattttgtc
    23221 aacaaataaa attccatcta agtggtctat ctcatgacaa aatgctctag ctaaaagttc
    23281 ttctccctct aactctattg tttcaccatt tctatttaaa gctctgacct ttacataatt
    23341 tggtcttcta acatttcccg cttctccaac aacacttaaa catccttcct catctatttg
    23401 ctccccagat gtttctatta tttcaggatt tataagttct ataagctctt ctccaatatc
    23461 tattactact actcttttta atattccaac ttgaggtgca gccagtccaa caccatctgc
    23521 atcatacata gtttctgcca tatcatctaa taattgtatt atcttttcat caattttttc
    23581 aacctttttt gatttttttc ttaatactgg ttctcctatt tgaactattt gtcttaaagc
    23641 cattataatt cctcctaaat ttataataca ctgtttggat taatatctat agatacattg
    23701 atattcttat caaatatcaa atctcgtttt gttatacata tatattttat tatacccttt
    23761 aagagattaa tttcaatatt ttcatcttta aagagtattt gccatctata attttgattt
    23821 atttttgata ttgaacatgg atttggacct aatataaatc ctaaatcatt tatacccctt
    23881 ttttctaata aataaatcaa tgatgcatgc atatttttta tattttttat cactaatctt
    23941 tcatcttcac cactaacaac tacacttaac atattattaa aaggagaata tccaaatgct
    24001 tttcttattt ttatctcatc ttcataaaaa ccctcataat catattcaat agcatgctta
    24061 atagcatagt gttcagtgtc ataagtctga agtactactt ttccctcttt atcagctctc
    24121 cctgctcttc cagagacttg agttatcaat tgaaatgtag tttcaaaact tttaaagtct
    24181 ggaaaattta atatcatgtc tgcagacaaa atacctacta aagttacatt ctcaaaatcc
    24241 agccctttac ttaacatctg tgttccaata agtacatctg cttctttatc tttaaattta
    24301 tttagtatct catctaaagc accttttttt gaagttgtat ctttatccat tctcaaagtt
    24361 tttatatctg gaaaaatata ttttaattcc tcttcaattt tttgagttcc aactccaaat
    24421 ggcttaacat aagtactttt acattcagga cactcttttg gaatttcttt ctcatatcca
    24481 caatagtggc atcttccagt attgcttttc ttatgataag ttaaagatat atcacaattt
    24541 tcacattgaa acacatatcc acactttcta caagatacaa aatttgcata acctcttctg
    24601 tttaaaaata gtattacttg attattttct tcaatcgcat aacgtatttc tttttgtaat
    24661 ttcaaactaa atatacttct atttccttta tctaactcat ctttcatgtc tacaacttca
    24721 atgtttggaa gaggcttatc atttgctcta ctttttatat ttataagttt gtattctcca
    24781 gattttgccc tataatactc ttcaattgag ggcgttgctg aacctaaaat aagagttata
    24841 ttatttttta atgccataaa tctagcaact tctatagcat taaattttgg attcttctca
    24901 gacttatatg atgactcatg acattcatca attattatta agcctaagga attaaaaggt
    24961 gcaaataaag ctgacctagc tccaattaac actcttactt tacctgcttt gacagctttg
    25021 taaacgtcat gtttttctcc ttcagatagt ttactgtgga acactccaac taaatctcca
    25081 aacctactct ttagcctatc tattgtttga ggagttaatg ctatctcagg tactaagaat
    25141 atactgtcta agccttgatt tagagcaaat tctattattt ccatgtaaac ttcagttttt
    25201 ccacttccag ttactccatg aattaagtat ggttttttat catctacaaa catattcgac
    25261 tttatctcat caactgcttc ttgttgctca ttgtttaatt ttatactttt tagtacggat
    25321 ttgtaaatac tttttggctc tctataataa tccttaaatt ctagggttat tagctttttc
    25381 ttacttagag aatttatact ttgttttgaa gcatttaata aatccaataa atcatttatc
    25441 tctacatttt cattattttt aagaaaactt aatatctctt tttgtttact tcccacattt
    25501 attttatttt gctctatata atcgtcaatc ttatcacttt ctaaactcaa agatatataa
    25561 catactttct tttcattttt gtgattttta tattcccaac ataattttat aattccattt
    25621 ttattcattg tgtacaaaga actatttaag tttggtatat attttaactt atcaacctta
    25681 attttacctt tactatcaat cactttctct actatttctc tatttttatc acttaaagtt
    25741 gatatttttt ccttcaattc aatatcattt aatccactta aactttcacc taataggact
    25801 actttataat tatttaattt gtatcccttt ggatatatca aatttataca ttctatataa
    25861 gtacacaaat atctattttt catccaataa accaactcta aatcttcttt tctaaatatc
    25921 ggattttcat ctaatatatc aataatttct tttattttta ttttttcatc taagctatct
    25981 gtaaattgaa atacaaaggc ttctgttggt ttattacctt ttccaaatgg tactaatatt
    26041 ctgtgcccaa tttttattac atcagataaa aatacaggaa tttgatatgt aaataaatta
    26101 tctgtatata tagtattact tctaactatt acctttgcat attttttcat tataaaacct
    26161 ctacttaaat ttaatatatt gttcatattt taatatactt tacaagatat taaaatctca
    26221 gaatttataa gcaatttcaa gttattaata ttaactttat tttaataatt aactattaat
    26281 tatattttat actttatgac aaaataaatg ctgaaaacta caatttaact gacatttgcg
    26341 agtttttact cgctaaaatt aaattgcagc tttcagccac tttagctgtg ataaaataca
    26401 caaatatcat atttaaaaaa ggtcttattt ctcaagcaac gctttaactt tatctaaaat
    26461 aacattggct acttcttctt ttttcatctt aggatactca gtaatatttc cctctacatc
    26521 tattattttt acaatattag tatcagctct aaaaccagca ccttctttag ttaaatcatt
    26581 tgccacaata aaatctaaat ttttcttttt tatttttcct ttggcatgtt ctataaggtc
    26641 attagtttca gctgcaaatc caattagtat cttatcttct tttatttttc ctatttcata
    26701 agcaatatca taatttctac ttagttctat tgataaatca tcattcttct ttttaatttt
    26761 tttgtcagaa taagtttttg gtttataatc tgcaactgct gcactttgaa ttactatttt
    26821 attttcactt aaatttgata ctactgcttc atacaaatct tgtgctgact ctatttgaac
    26881 aaatttttta agattttgtg gaatagaaag atttgtagga cctgatatta atgttacatc
    26941 agcacctcta gctatagctt ctcttgctat agaatatccc attttaccag aagacctatt
    27001 agttaaatat cttactgggt ctatactctc tacagttgga ccagctgtaa ctatcatact
    27061 ttgacctttt aaatcttgct ctttagtaag attagtcaaa acttcctcta ctattatatc
    27121 tggattagct agtttacctg ttccaatatc tccacatgca agccttccac tttccggttc
    27181 aataaattta tatccaaagc tctttaaagt ttgtatattt ttttgaacaa ttggattttc
    27241 atacatatta gtattcatag ctggtgctat aagaacctgt gcttttgttg ccataacagt
    27301 agttgtcaac atatcgtcag ctataccatt tgcaatcttg cctataacat ttgcacttgc
    27361 tggcactatt aagaataaat ctgctttttt tgctaatgat atatgttcaa cttcccaagt
    27421 ttttggttct tcaaacatat cttctactac ataattttga ctcagcgatt gaaatgtaag
    27481 tggtttaaca aattctgttg ctgattttgt cattataaca tggatatttg catttagttt
    27541 ttttaactta cttacaacat cacaagcctt atatgcagct ataccgccac ttatgccaat
    27601 tacaacagtt ttatctttta gcatgacctt actcctcttc tgaaatttgt tggtgttgct
    27661 cagcatgatg tctagcttct tctatctcta tttcctcttc tgtcaataat ctatatgtta
    27721 tttttccact tgcaacttct ttagttgcta cacatacagg cttttcctta gttttatttg
    27781 aaacatatgg ttcttctcca tcaattaatt ttcttgctct ctttgaaaca gtccccacta
    27841 aatagtatct gttgtctatt ttttctagta cctcattgat agatggtttt aacataatta
    27901 taattcctcc ttaaatttct ctattataat atttttatat ctgctaactt tattcttttc
    27961 agatgatatt ataccttcta tttctttagc agatgttttt acatcttcat taaatataaa
    28021 ataatcataa tccttaatca ttttaatttc ttcatatgca cagctaaatc tcttctctat
    28081 ttcagcttct gtttctgtac ctcttccaac aattctattt tttaattctt caagtgatgg
    28141 tggtagtaca aatataaata ctccctctgg gcaaacttct tttatttgtt ttgctccttg
    28201 catttcaatc tccagtaata catcttgtcc tttttctaaa cattccatta ttgccgcttt
    28261 tggagttcca taaaaattat cataaatttg agcatactcc aaaaactcac ctttttctat
    28321 catctcttca aacttttcct tacttatgaa gaaataattt actccatcaa cttctccagt
    28381 tctgggtttt cttgtagtag ctgatacaga aagttttatt gtatcatttt ctttaagtag
    28441 ttctttgcaa atagtacctt ttcctgcacc tgatggtcct gatacaacta gcaataatcc
    28501 ttttttcaca aacatttaag cattcccctt ctaatattgt attcttactc tatattttgt
    28561 atttgttctc ttattttttc caattcgctt tttacttcta ctactaaatt agttatattt
    28621 atatcagatg acttcgaacc tatagtgtta gtttctctat tcatctcttg tattaagaaa
    28681 tctatttttc tacctataga gtcgtcttta aatatagtat ttttaagttg ccctatatga
    28741 cttttaaatc taactatttc ttctgttata ctgcttttat ctgcatatat tgcaacttct
    28801 tgtgctaatc tattctcatc tattatactt gggtcatcca gtaattcact tattctcaaa
    28861 ttcaattttt ctctataatc ttctacaaca ctacttgaat atttttcaat ctcttctata
    28921 tgatttttta ataaatcaca tctcatggca atatcttcag ctaatttttt accttcttca
    28981 cttctcattt ctcttaattt gattaatgca tcttcaactg cttgattcaa cattgaccac
    29041 aataaatctt catcttcttc cttttcttct attttcacaa tatcaggaaa ctttgcaaca
    29101 ttcataacgc taatatcatc tataatatca aatttatttt ttatttcttt tagtatgtct
    29161 atatactgag atgctaatcc ctcatcaaaa tttaagttta catcttcttt gcctaataag
    29221 tcaaacttta tgtataaatc tactcttcct ctttttacat aattttttac taaatttcta
    29281 actttatcct ctaaaaatga aatttttctt ggaagacgta tgtttatatc tgaatactta
    29341 tgatttatcg ttttacactc tactaaaaag taataattat catccttgta ttctcctctt
    29401 ccaaaaccag tcatacttat agccattaat taaacctcca aaataccttc acatattttt
    29461 actgctggac ctgtcatata agagaaatct cctacatcta ttttaactgt tccaccttga
    29521 gacgttacat ttacactact ttctaccata ttaagtaaat tgcacactat tgctgatgca
    29581 gtcataccag taccacaacc taaagtatat ccacaacctc tttcccaagt ataaacatgt
    29641 atattatact tatcttctac ctttacaaaa tttacattcg ttttttcagg aaatatttta
    29701 ttattttcaa tctcttttcc atatttacaa acatcatcta catctaattc atctactata
    29761 actactgtat gaggaacccc catcaacata gaagtaactt taagctccct atctaaaacc
    29821 tttatacttt cttgcacaaa acgttcttta tttgtaagta caggaacgtc cttagctata
    29881 aaacttcctt ttcccatatt gactcttata gagtttattt ctctattagt aagatttact
    29941 tttatatctt ttataccatc taatgtatct actgtaaaac tttctttttt aactatacaa
    30001 ttatcataaa caaatttaca aaaacatcta aggccatttc cacacatagc tgctcttgaa
    30061 ccatcagaat tataatatac catttctaca tctgcaactt ttgaatcttt cacaactaac
    30121 aatccatccg ctcctacaga aaatcttcta tggcatactt tttttgctaa agcagtataa
    30181 tcagatggat ttatctggtc gaatcttcca tctattgcta taaaatcatt tcctattcca
    30241 tgtagtttcc aaaatttcat aagtatcgct ccttcgtaaa ttaacgctta aactattata
    30301 acataaaaaa attcccataa acttataatt tatgggaaaa gttttgtaat gacaactata
    30361 atttgaggat gtgagggaga gttgccatta caattagggt aattatatta tgtttttaag
    30421 ctagttcttt ctatgtttat atattaatat ttctatatga attttatgta tgttttattt
    30481 aaactgtcta tgaatttttt aattaaacat ctggtaacta gctataatcc ttctagtaga
    30541 tatctattaa catacaccta ttaattcttt aagtgttttt aaaacatagt ctaaatcttc
    30601 cttagtaata tctacatgag ttacaaatct atattcacca ttttccattt tattaatttt
    30661 tatatttctt tcaaaaaatt cttttattaa aacatcctct tttataatat cttcactcat
    30721 cttaaaaaat accatactta tatctggatt aacattgttt acttcaatgc catcaatttt
    30781 atttaattca caagctaaat attttgcatt ttcatggtct tcattcaatc ttaaagtcat
    30841 tttttcaatg gcaattatac cagctgctgc aactatacct acttgtctca ttcccccacc
    30901 cataactttt tgctttcttc tagcttttgc tataaattct ttatttccag caagtataga
    30961 acccattggt gcagctaaac cttttgacac acagaacata acactatcac aacactttgc
    31021 tatttcttta gcttctacac ccaaagttaa tgctgcgtta aataatcttg ctccatctaa
    31081 atgtactggt aagttatgtt tctcagctat ttttttaatt tctcttaaat tatcaagact
    31141 cacaactgtt ccacctgaat atgcattttc tacacatata agccctgtct gtggttcatg
    31201 tatgtcatct cttctaattg ccttttcaac aagttcagga tttagagctc cactttcgtc
    31261 ttcatctata gttctaagct gaactccagc tattacagca ggtgccccaa cctcatgagt
    31321 cacaatgtga taacctttac caataattat ttcttgccct cttaggcaat gagtaaatag
    31381 agctaattga ttcccaaaag ttccacttgg aaggaaaata gcatcttctt ttccaacttt
    31441 agctgctgct aattcttcaa gtttatttac agtagggtca tctctaaaga ttgaatctcc
    31501 aacaggagcc tttgccatag cttctctcat ttcgtcagta ggcatagtaa cagtatcact
    31561 tcttaaatcg ataaatctca ttttttcaac cttctttcat atttatatac tatagaacct
    31621 taaaattgat tttttctgta aagttcatat actttcatag cttatactct acacaataat
    31681 tatactatat ttactttata tttaatctac tttaaattat taattttaaa aataaagttt
    31741 cctctctaat aaatataaat aattatacaa tttaaatatt aatctctatt gtttaaattc
    31801 cccctagtca caaagcgtat attatgtgta taattaattt actgactcat tttggtcaaa
    31861 gaaaggggtg ctataaatgg cattagatgg attagttata cattcaatag tcgatgaatt
    31921 atcatcaaaa cttacaggtg gaaaaataga taagatacat caacctgaag atgatgaggt
    31981 aatttttaat ataagaaata ataaagaaaa ttttagattg gttttaagtg caagtgcctc
    32041 aaatcctaga gtttatctta caagtaatta tcaaaaagaa aaccctctaa aagcgcctat
    32101 gttttgtatg ttacttagaa aatacataca aggtggaaat atagtagaaa tttctcaaat
    32161 aggttttgaa agaataataa aaataagtgt tgaatcttta gatgaattaa aagaaaagac
    32221 agttaaaaat attatgattg aaattatggg aagacatagc aacataataa taactcatgg
    32281 tgaagaaaat aaaattatag attctataaa aagagtccct tttagcataa gtagagttcg
    32341 tcaagtttta cctggtcatg attattcatt gccaccagaa caaaacaaac tcaaccctct
    32401 tgatgatata tctaaggatt tgtttattaa aaacctagag gaattggaag gacctatctt
    32461 taagtctata tattctaaat ttctaggtat aagccctatt atagctaaag aaatttgcta
    32521 cagagcaggt gtaaatcaaa atgctatcat aaaggatata tctgatgaac aatttgatgc
    32581 tttacacaaa gtattttgta atttattcaa tgatataaat tctaacaaat actctccttg
    32641 cattattata gataaaaagg tggataaggt agttgatttc agttgtataa accttacatt
    32701 atttagcgat ttatcatata taaataagga tagtatgtct cgtattctgg aagatttcta
    32761 tagaactaag gatattaaag atagaattaa tcaacgttca tctgatttaa agaagagtat
    32821 atctgttaag cttgatagac tttataataa acttaaaaaa caagaagaag aattatcaga
    32881 atccgaaaat gcagacattt ataagataaa aggagaattg attacttcat atatttatat
    32941 ggtagaaaaa ggtatggaaa gtattgaagt tgctaacttc tatgatgaaa attgtaatga
    33001 tgtaacaata gaattaaata aaaacttgac accatctgaa aatgctcaaa aatactttaa
    33061 aaagtataac aaaatgaaac atgctaaagt cgagatatct catcaaattt ccttaaataa
    33121 ggaagaaata gattacttag aaaacataat tttaagtatt gaaaactgtg aaaatttagc
    33181 tgaattacaa gatataaaag aagaacttgc taaagttgga tacatcaaga ctcaaaagaa
    33241 aaacagtaaa aaggatacta tcccatctac aaaacctcat gaatttgtgt cttctgatgg
    33301 atttaaaata ttggttggta aaaacaataa gcaaaatgac tatcttacac taagacttgc
    33361 tgataatgat gacttatgga tgcatacaaa aaatattcct ggttctcatg taataataaa
    33421 gtgtgctggt aaggaagtcc ctgataatac tgtatttgaa ggagctatgc tagcagcatt
    33481 ctttagtaag tcaaaacttt cttctcaggt gcctgtagac tatactaaga gaaaaaatgt
    33541 gaaaaaacct agtggttcta aaccaggtat ggttatttat gaaactaaca gtactattta
    33601 tgttactcct gaagaagaaa cagtagccaa gcttaaggtt aaatctgaat aaattctgca
    33661 ctaaatacaa aataaatgtt agacaactac acattatcat tctaaataaa aatagtggcc
    33721 ttcttataaa gtacaataat aaggccactt tctaattagc ttacttttaa ttttctaaca
    33781 aaatactttt ctgttttaat aactatactt atattcccaa caaatatttt cagtcttctt
    33841 atatagatgt aattaaaaag ttaacactaa aagctatttt ttaatttatt gatttgtttt
    33901 agtaattcat tatattctga ttcatacaat tcttttttta gattatcttt ttctatagat
    33961 aacatagata taacagttgt aagtctattt tctaatacta gaagattttc ttttttgatt
    34021 tggttttctc tactaggagt ttttttattt ctgctaattg tatattcgtc ataattacaa
    34081 tcaaactccc ttattttagc atcttttatc tctataatat agttacaaat atgtgataca
    34141 aatactctat catgagacac tataagcata gttttatcag tatttatcag tgccttttcc
    34201 agtgcttcca tagattttat atctaagtaa tttgttggtt catcaaatac taaaaaatta
    34261 ttatcttcca atattatttt acatagtgct atttttacct tttctccacc actaagtact
    34321 tttacttttt tgtagacatc atcaccttta aacccaaata ggcttaagtt tatccttata
    34381 aaagattcat caaatgaact attaacttta gtattgtata gaacactctt ttcctcatct
    34441 aataaacttt gattttggtc aaaataacct acttttacct tgttatttat ttttatattg
    34501 tcatttttat cagccaatat ttcttttatc agagtacttt ttccacaccc attatcacct
    34561 aacaatgcta tctttttacc tctttttatc ttaaaggaaa cattatctaa caggagctta
    34621 ttttctaact ttagagtcat atcttttacc tctactagat ttttgctaat tatctccata
    34681 ccatcttgca tcttaatgtt catttcttta gaaactttag gtttattctt tacctcaagt
    34741 ttatcaattc tgctttttat agccttcaca tttgagtcca actttttctt attagtctga
    34801 ccacccattt tgtgcaacct agcttctgaa ttacccattc tttttggtgt ttttcttatg
    34861 ccatcactta aagctttttt atataaaaga gctttttcaa gccttttttt ctctttaaca
    34921 tattcttgat actcaaaatc agctcttttt acttcttcat ctttttgcat taaataatca
    34981 gtgtaattgc ctttgtaagc ttttagtttt ccatcttcta gttctaatat agttgtacat
    35041 aactcatcca gaaaacgtct atcatgagat attaataata atgctccctc atgcctttta
    35101 aacatatctt ccaaaacccc aatgcttttt ttatctaagt ttgaagttgg ctcatcagct
    35161 attatgatat ttttcttatt actcagcgca tctgctattt ttaacttaac cttttcccct
    35221 cctgataagt gttcttcaaa tttatctgga gcatttaaaa gactcttctc cttactatga
    35281 ctacatgttt caatattatt ttcactctga cttatataag aatagctttc tgtaaggtaa
    35341 gtatatcctt catcaatttc tatttctccc aatattgctt ttaataatgt tgtttttcct
    35401 tgaccatttg aaccaactag acctattttg tcattttcta atatttcaag tttatctata
    35461 tccaaaatta atttatctgc ataatatttc tttaaattct ctacttttac taacaacata
    35521 aaaaatccct ccttaacaca cttaaatata aggagagatt ctataaatag gcataacaaa
    35581 taaacgtata gactataatc tactctcctt aatttaagca ttgcaaataa agccaccaaa
    35641 ataggctttt caaaaattac aatactttaa attataaaaa atagattata ttctcattgt
    35701 cctagttacc ttgccctttc tttcatttat ttttaatcat tatatcaaca ttaattacaa
    35761 atgtctatag aaaattaact aattttagtt attcaatata caacttattc tttaaattta
    35821 ttcttcttca actttaaagt cctgattatt taaaattctg tttaatacaa ttccaacaat
    35881 tgcagctaaa ctaagaccag ttatctttac tgtacttgtt atagggattc caacagaaat
    35941 acctttatta gataaatatg ttgttccaag acctattaat aatataactg ctatgattaa
    36001 tatatttatt ttactttcta cacaagaact gtcccttata gtttttaatc ctacataagt
    36061 tatcatcgaa aatagcatta tactaattcc acccataact gaacctggta tactttgaag
    36121 aaatccacca aacttgccta cacatgcaag caatatagca aatattgctg tacgtctaag
    36181 tattgaaggg tcatagtttt tagttatagc aagtactgct gtattttctc cataagttgt
    36241 attggctggt cctcctaaac accctgcaac tatagtcgca aatccatccc caagtaatgt
    36301 tctatttaat ccgggatttt ctataaagtt tttacctact acagcaccat tagtagtcat
    36361 atctcctata tgttccataa atacagctaa tacaactgga gatataatca ctattgctcc
    36421 taaactgaac ttaggaactg taaagttagg tattgcaaat aaacttgctt ctgtaatagt
    36481 agttatatct actaatctta atatcaaaca gattatatac ccagaaaaaa ctgctattaa
    36541 tattcctaat tgttttataa atcctctacc aaacttattg gttaaaattg ctattgctaa
    36601 agttataaat gctatcacaa aattggctga agccatacta aaagctgttg gcaataaatt
    36661 taatcctata actactatca tagctcctgt aacttgtgca ggaaaatata atttaatttt
    36721 atttacacca actactttta ctataatcga cattataata taaattaatc ctgctactat
    36781 tataccacct tgagcatatg ctaaatctcc accatatgcc tctttagctg ctagtataac
    36841 tggtataaac gcaaaacttg aacctaaaaa ggcaggtact tttccttctg tacaaaaatg
    36901 aaatatcaat gtaccaacac cagcacagaa aattgcaact gacggattaa aacctgtcaa
    36961 aatgggaact aatactgtcg ctccaaacat ggctaataga tgttgtaaag acattatcgt
    37021 ttttttcata aaaacctcca aaattattat atataaataa atcctctgtt taattaatat
    37081 cttttaatta attatcattc cctagattct attttttaac ctctatatac ttattatata
    37141 atttatcatt attttacatt ttacgacatc aaaaactcaa ataaaggaga ataaaaacta
    37201 cattaagact cacatccata aaattataat atttattttg tgcaatctat gtaagcttta
    37261 tcctcctccc tgactattca atgctaagct tttatataat acttaaaaat aattattctt
    37321 tgattgtaac tgaatcttct ccatcaattt ctaataaact gacagatata atttcatgtc
    37381 tagaagtagg tacatttttt cctacataat ctgctcttat aggcaactct ctatgccctc
    37441 tgtccactag cactgctaat tgtattgatt taggtcttcc tatatctata atagcatcta
    37501 gtgaagctct cacagttctt ccagtgtata aaacatcatc aactaaaact actgtcttat
    37561 catttacatt aaaatctaaa taagttcctt taactacagg ttcaacatga attttttcca
    37621 aatcatctct atacaaagtt atatcaatat ctcctgtatc tacttttgtt ccttctattt
    37681 gttctatttt cttagaaatt ctatttgcaa ttggcacacc tctagtcttt attccaacta
    37741 aaactagatt ttctacacct ttatttcttt caattatttc atgacttatt ctagtaattg
    37801 ctcttgctat tgccttttca tccattaatt gagctttttc taccatatca taattattgt
    37861 caaagatata atatatctat ttgacaaatc tactcctttc ttttaatttt ttataaattt
    37921 atttttattt aaaatttcaa tttaaacaac aaaaaaactt ctcatcacaa gacaagaagc
    37981 tcttatataa aaaatataca gtgcaattta gcaattgtct tacgatatct ctgtatcgtt
    38041 taaagataat tttttaatct atcaaatcaa attatatatt tatatttcaa atttttcaag
    38101 cttttttaaa caaaatactt taatataaga tattttattt aaatttcact aaatttattt
    38161 acttatataa aaacaaaaaa tgatttataa gtattataca aatagttatt ttatcttttt
    38221 aataacttcc ttaaaatact caggtaattc tgaattaaat tcgatatatt ccttagtagt
    38281 aggatgtata aatcccaact tcttagcatg caaagtttgt ccttttaatt taaatttagt
    38341 ttcttttgga ccatatacat aatcacctaa aagtgggtga tttatagaca aagcatgaac
    38401 tcttatttga tgagttctac cagtttcaag cctagctctc atatgtgtaa acttatcaaa
    38461 tctttctaca acttcaaagt gagtgattgc attttttcca tcttttacta cagccatttt
    38521 taatctatcc ttaggatttc tccctatagg tttatcaaca gttattttat cttctttaac
    38581 cacaccatag catataaatt catactccct agttatagta tgttccttta actgttctgc
    38641 tagaaaatta tgtgcataat tgttcttagc tatcataaga agaccagaag tatctttgtc
    38701 tattctatga actataccag gtctaataac gccatttatt gatgacaact tatctttaca
    38761 atgatataaa atcccgttta ctaacgtatt ttcatagttt ccaggagcag gatggacaac
    38821 catatcctga ggtttattta ttattaagac atcatcatct tcatatacaa tatctattgg
    38881 aatatcttga ggtaaaactt ctaatacttt aggttctggt atctctataa caatattatc
    38941 ttcctcttta accagatatc tagctttttc tactttatta ttaaccttta cttttccatc
    39001 ttttataatt ttttgtatat aacttcttga catatcacct aattgttctg ctagataaat
    39061 atcaagtcta acttcttctt cctcttcaag tactaaaaac tcttttactt catccattat
    39121 atcacctact tttactttca aaaaataaaa catatataca taaaaacaca gttcctacaa
    39181 ctacaaatac atcagctata ttgaatacat attcccatat aattctaaag tcgaagtaat
    39241 ctactacaaa acctaatcgt actctatcaa ttagatttcc caatgcacca gatataatta
    39301 atataattcc caaccttcca aatatatgta ctttccttgt attaagatag tatagtccaa
    39361 atactgttgc aagtaatgca acaattataa atatccattg attattttgt aataatccaa
    39421 atgctgcacc tctattttcg acataagtta aatgaaatac attatttatt attggtattg
    39481 ttgatacatc caccaaatta ttcaataccc atattttaga cagttggtct aaacctatga
    39541 gtagaattat tattaatata tatagcaatg atttttcccc ctataaatta tttaaaaacc
    39601 aattttattt gaagcacact attattatta tacaaaaaaa atgctctaaa gagcaacttt
    39661 attaaaattg tattttctta ataaagctag aacaaaattt aaaatagt
//
LOCUS       DAEPIN010000030        38753 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.030, whole genome shotgun sequence.
ACCESSION   DAEPIN010000030 DAEPIN010000000
VERSION     DAEPIN010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 38753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38753
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.030"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            complement(<1..741)
                     /locus_tag="KR250_001798"
     CDS             complement(<1..741)
                     /locus_tag="KR250_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBH0067734.1"
                     /translation="MEYTVQKLSKLAGISTRTLRYYDEIGLLKPLKINSSGYRIYGQN
                     EVNKLQQILFYRELGINLENIKNIINSPNFDSLSALKEHHNKLLAKRKQIDLLIENVT
                     KTIALKEGKYTMTDIEKFEGFKEKMIDENEKNYGTEIREKYGKDIIDTSNKKLKNMSK
                     QDYENWQNLNIEIISKLKKAFKTGDASSELAQEVAKLHHKWLSYTWNTYSKEAHSSLA
                     QMYVYDERFTSYYDREQPGLAKFLRDAIV"
     gene            960..1436
                     /locus_tag="KR250_001799"
     CDS             960..1436
                     /locus_tag="KR250_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HBH0067735.1"
                     /translation="MNLKRSNEKREEYRRMYSDKESFLSLIQNFTSVSIAKELTLKNI
                     ELETSFICEYKGKEVDIIYKVFSKSGKVSHYIVLEFQTEMDTEIVPRLKSYREQIWKS
                     FIMKKSLEEIEDKNFKLPKVIPVVLYSGPERLSRKKGILDIVESISEFSNNSSMKN"
     gene            1534..1686
                     /locus_tag="KR250_001800"
     CDS             1534..1686
                     /locus_tag="KR250_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate translocator"
                     /protein_id="HBH0067736.1"
                     /translation="MRNNKSKVLLGILCLATGMAVILSMLLPGWIWSALTALILIGCG
                     ALLFFC"
     gene            complement(1846..2034)
                     /locus_tag="KR250_001801"
     CDS             complement(1846..2034)
                     /locus_tag="KR250_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HBH0067737.1"
                     /translation="MAKVCSVCGKGKVSGNQVSHSNKHNKRTWSANLRSVRAIIDGAP
                     KRVKVCTRCLRSGKIERA"
     gene            2395..2745
                     /locus_tag="KR250_001802"
     CDS             2395..2745
                     /locus_tag="KR250_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBH0067738.1"
                     /translation="MSAKVTNQYGNIEIDNQVIAQVVYRAAMESYGLVGLAYKSKGIV
                     ELLKGENATKGVRVEEVEEDAIAIELYVIIQYGTNISTVANNIIDRVKYVVEKMTGVR
                     VTRIDINVQGIRVK"
     gene            2764..4407
                     /locus_tag="KR250_001803"
     CDS             2764..4407
                     /locus_tag="KR250_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HBH0067739.1"
                     /translation="MIQYIDGKRLREMFISGANNLQNNKELVDKLNVFPVPDGDTGTN
                     MSLTISYALKELAKVENDNISDIGKALSKGSLMGARGNSGVILSQIIRGIAKSIEGKS
                     KLSTEDLAKAFKNGSDTAYKAVIKPIEGTILTVVRESGEFAIKTAKKEKDVVKFLSML
                     VKESNSSLERTPDLLKNLKEAGVVDSGGKGLVLIYEGMLASIKGDNIEIKNVDLDTNI
                     STSMDFAKSTTSTDDIKYCYCTEFILESSKVEDTKIRDIMMAYGDSLAVVGDDGVIKV
                     HVHTNDPGNVLQEALKYGQLLTIKIENMKLQHENTLLDVEEKKENDSEPLEEEKEFGF
                     IATSMGEGLANIFKDFGVDHIIEGGQTMNPSTEDFMNAIKHINAKNIFIFPNNSNIIM
                     AANQAKELSDKNIIVIPTKNTPQGFAALVTFNGELSEDENKEAMMNALNSVKSGQVTF
                     AVRDTVMNEIDVKEGNIIGIAEGNLLSAGDYVDEVTSNLIEKLVDEDTAIITLFFGED
                     VTESQANELRTSLEEKFEDVDVELYYGGQPLYYYLISVE"
     gene            4488..6545
                     /gene="recG"
                     /locus_tag="KR250_001804"
     CDS             4488..6545
                     /gene="recG"
                     /locus_tag="KR250_001804"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HBH0067740.1"
                     /translation="MDLYKDVQYVKGIGPKKADKLNKLGIFTLKDLLYYFPRQFEDRN
                     NLKKIAQLEDGEKVTIKAVISSINTFSPKEGMTLTKIDVKDETGSAKLVFFNKSYIKN
                     TFRPGDSILVFGKVKKKFNNLELTSCELEYLTNSPKNTCRFMPVYQLTYGVTNKEIMS
                     IIRTVLEDKELIIQEYMPQRIIEKYRLCSIDFAVRNIHSPSSKESLKIALYRIVFEEL
                     LILQLGLFVFKSGRNKEDGIKFETSKDLKKIISALPFKLTKAQNRALDEIIQDMNLEK
                     IMNRLVQGDVGSGKTVVALLALANCVLNGYQGALMAPTEILAGQHYISLTESLKDFGI
                     NVGLLIGSLTKKQKDTVLEQIKNNEIDILIGTHALIEDKVEFNNIGLVITDEQHRFGV
                     MQRSKLSLKGANPDILVMTATPIPRTLALILYGDLDISIIDELPPGRQPIETIAIEKS
                     KRDRAYNNLVRREVESGRQVYIVCPLVEESEAIEAKSAVELVEELRAEYFHDLRLGLL
                     HGKMKSSEKDEVMRLFKDKEIDILVSTTVIEVGVNVPNATLMIIENAERFGLAQLHQL
                     RGRVGRGSHKSYCVLIYDSKTDVCRQRMAIMEETNDGFKISEKDLEIRGPGEFFGTRQ
                     HGLPELKVANLFKHIKILKLAQQEARYILGEDNNLQLKENMALKKEIIDKFKDTLKEI
                     SLN"
     gene            6604..7158
                     /gene="rsmD"
                     /locus_tag="KR250_001805"
     CDS             6604..7158
                     /gene="rsmD"
                     /locus_tag="KR250_001805"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HBH0067741.1"
                     /translation="MRVISGKARGLKLNTPKNEDVRPTTDRVKESLFNMINSYIMESE
                     VLDLFAGTGSLGIECLSRGAKSCTFVDISKESIDIVKSNIKKARVESESIILNLDFKT
                     AIDKLKLQNSKFDIIFMDPPYYKNMFIEAIEKIDNSNLLNEDGIIVVEHDTNDLFPDK
                     ICKLEKTRDKKYGNTTLTFYKMEA"
     gene            7160..7657
                     /gene="coaD"
                     /locus_tag="KR250_001806"
     CDS             7160..7657
                     /gene="coaD"
                     /locus_tag="KR250_001806"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HBH0067742.1"
                     /translation="MENKPRKAIFAGSFDPITNGHLDIICRASKLFDELQIGVLNNPN
                     KKGLFSFDERVKLIEKSTSHLNNIKVVTFDGLLINYCQENGIGALVRGVRSGADVDYE
                     LQMAHMNRELNPDIETIILPSCTKYSFISSSLIKEVLLFDADIKNLVPKIVLEELKKK
                     TSGGN"
     gene            7661..8185
                     /locus_tag="KR250_001807"
     CDS             7661..8185
                     /locus_tag="KR250_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0067743.1"
                     /translation="MKIDLEMIELFEQLEEIFRSASTIPFSHKCTVDKDEVLAIINDI
                     RTLIPEEVTQAVWINKERNKIISQAKQDATNIVEQAKKEAERIQQEYQDNIEELKKNS
                     EDVVKAYVESSEPVVQADQRAKDIVDRAERIANEIRIGSIEYAEDVLSSVEYNLREIL
                     EEVKRDKAELRPKK"
     gene            8529..10448
                     /locus_tag="KR250_001808"
     CDS             8529..10448
                     /locus_tag="KR250_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBH0067744.1"
                     /translation="MKERAKKILDFIIKNNNVTSQELQEKFNVSKRTIYYDILAINEQ
                     LGKSGNIKNVKHKFIFEGNLCDARKIISTEEDKFLDSDYRKTFILNKILLGEKISIEK
                     LTNEMLLSKNTVVQTITDVKKYLQTMGLRLEYKGKYKIIGDEYVIRELFLIIVQENVL
                     EINSISEEVSSFDTKGHIKLTDYSLLNLTKFVEFLNKRIRDGKTLYSYKYLNEAKKIS
                     YFSNCKELLCEEANENEQAYICTYISSLPSLNSEVKEDVVEEYVDKLIDKFEVNTAIK
                     LESKHEFKKNILRHLHSSYNRIRFKFPIRNPMLDETKYKHESLYKIIKSIIENEEEFP
                     VFEGIREEEIGFIAAYFGGYLRGSRDNGLRRNKVLLVCPNGLMVSKSLEIQLYKYIPT
                     IEIVGIVSIKQLKEVNVYYDYIITTIDIQNVNNVIVVNPLLTSSDVQLLMNKLISVKE
                     NEKYFNLELIIQAIRKNGVINNEEALKADLLNIIHKIDEGEMYQPMLKELINAERVNI
                     IKNVRDWKEAIKIASKPLLEDNSIEELYIENMIKSVEKYGPYIVLADRFALPHASSKE
                     GVNKLAMSLLIVEDEVDLLGKPVNIFMVLAAVDNTTHIRALASLSEMMYEEENVKLII
                     NGDKSSIIELINKQN"
     gene            10461..10748
                     /locus_tag="KR250_001809"
     CDS             10461..10748
                     /locus_tag="KR250_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBH0067745.1"
                     /translation="MKILAVCGFGVGSSMVLKMTIQKALKQLGIECEVENTDINSARG
                     ADCDCIFTSQELLNELKGTCNVDVYPVKKYMDLAEVTEVVQTFLSDKNDKK"
     gene            11516..11629
                     /locus_tag="KR250_001810"
     CDS             11516..11629
                     /locus_tag="KR250_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013483530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBH0067746.1"
                     /translation="MIMGRHLWNPSYFVATVLENTEEQIGKYINNQKKQVK"
     gene            11631..12749
                     /locus_tag="KR250_001811"
     CDS             11631..12749
                     /locus_tag="KR250_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBH0067747.1"
                     /translation="MIVVEKAYKFRMYPNKKQQELINKTFGCCRFVYNKYLAKRIEVY
                     KNDKETFTYKQCSSDLTNLKKELKWLKEPDKFSLQNVLKDLDNAYKKFFKEKAGFPKF
                     KSKKINRFSYKTNFTNGNIMYCGQHIKLPKLGMVKIRDKQVPQGRILNAAISKEPSGR
                     YYVSLCCTDVDIEAFENTNNQIGLDLGIKEFCISSCGEFIENPKYLKKSLNKLAKLQR
                     ELSRKTIGSLNRNKARLKVARLQEHIANQRNDFLQKLSTKLIKENDIICIEDLQVKNM
                     IRNSKLSRLISDVSWSEFIRQLEYKANWYGRQIVKVGKFFASSQICNKCGYKNEEVKD
                     LNIREWICPSCNETHDRDINASINILKEGLRLITIQNK"
     gene            12900..14117
                     /locus_tag="KR250_001812"
     CDS             12900..14117
                     /locus_tag="KR250_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HBH0067748.1"
                     /translation="MLIGLIAAFGLVLQKKKASDVIKGSLLAAFGIIIMETGTGMLVS
                     SIAPINGAFQSISGGAVTKGLSDVTFTASYGGIIGLAMFAGLAIHLMIARFTPIKTIF
                     LTGHMLYWFPFIFVASGVEAGLNGGVLITFAAVLSALYWSIMPWLLRKYVFDVIGDDS
                     FTLGHPSGCLALISGFVAKLVGNKEKSTEDINIPQSLSFFREISISGGMAVFLMFIVV
                     GIVIPGAFKDSNEFIGTYAIKQGLSFGAGLIIMLHGVRMLINQIMPAFQGISEKVVPN
                     ALPAFDCPILFNYKPNAVLIGFLVAMLTSTAMILIYNYFNVFNLLLIPLVITSFFECG
                     AAAVIGESQGGIRGAIIGTAIAAIFMVVLVGISAVIYSGTIQNWILIFGGNDLSLFGI
                     ISRFIGSLFGGIL"
     gene            14120..14860
                     /locus_tag="KR250_001813"
     CDS             14120..14860
                     /locus_tag="KR250_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBH0067749.1"
                     /translation="MKFKYIDEIRRLIDIIENEEKVNMEKAVQIFVEAIESKNAIFSF
                     GASHAGILTEELFYRAGGCVLINPVFAKSLMLDTAPITHTSKMERLEGYGTALADKTP
                     IKKGDVVIVHSVSGRNPVSIEFAMKAKENKAIVICITNLSYSKSVSSRHSSGKKLYEV
                     CDLVIDNHGQKGDACVEIDGLNQKVSPTSTVIATTVMNSIIAQATQELVNKGLKNPPI
                     FYSANIDGGDELNKKIFDEYKSVIHYEY"
     gene            15183..16493
                     /locus_tag="KR250_001814"
     CDS             15183..16493
                     /locus_tag="KR250_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBH0067750.1"
                     /translation="MKKENKMEGIKVGMLKKLCSLAMVAIMTTTLITGCSSGNGKDSK
                     KDGEKEKLVLWMPPVENNMKEVWDPILDKFEEKNNCEVDLQIIPWDNYSEKFATAISA
                     GEGPDVGYMYAEMYPQFIESGAVEDLTNYATKEDKEQSIYIKTSEMMGGMYGMPFQAA
                     NPGVLYYNKDILEKLGEKPPKTWEDFKRICKKATQDTDGDGKIDQWGLAQGWGAKTFG
                     NLNWNWYPYLWQAGGDIFNDDLKSVKFNDKSGLEAANFLKELQAYVPEDSLSKDSNEM
                     IESVFGPGKAAFTIMLSSAATSVFDESFPDLNWGFVTGLEDKKAATFGAVDHLSLMSS
                     AKNKELAYKLIQHMLSVESMTEFHKAIPRAPITKGEPYQGDERFKEMVENDKNVYRPL
                     VVGPHGVEIYEYLWKELQTMISGDKTPKQALDDAAKYSNDLLAQ"
     gene            16591..17508
                     /locus_tag="KR250_001815"
     CDS             16591..17508
                     /locus_tag="KR250_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBH0067751.1"
                     /translation="MENLVREFEENSKRKKTKLNIKPYMYILPLGIILVAFYVIPIIM
                     SIYFSFTKYNIISPATFIGLENYKKLFTDETLKVSIINTIKFTVVVVPCQTILSLILA
                     VWITGKGNSKIASFAKGAIFIPVLSSMVLIGMVWRALLNGEGSIIYQVLGAFGIESSK
                     LLGDSKTALPTLMFISMWKNIGYFMVIYISAIMNLPKHCYEVAKVDGATKLQEFIKIT
                     VPLLKPTTIMVVFLGSIWSLQVFDLIYTVTGGGPGISTMSIVMHAFNLNFKNFNSGYA
                     MTVANVLFLLIAVVSILQNKLVRRDNSDF"
     gene            17524..18333
                     /locus_tag="KR250_001816"
     CDS             17524..18333
                     /locus_tag="KR250_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBH0067752.1"
                     /translation="MKTARRLLSDGLLLFIACASLVPFIYMLIISLKITYNSYSLDIS
                     FSTVTLQNYIDIFTKKGFAQYFFNTAIVSFSGVLLNLVFSTLAGYSFAKMDFKGSDKL
                     FLFMIMTLIIPSQVTMIPLYIIMKHLGWINSYLALIMPIPTAFGVFIMRQAILGVPKE
                     LLESAKIDGCSDFRILIQIVLPLIKPALITLAIFTFMGAWNEFMWPLIATTKDTMRTL
                     TVGLSTLKTFQITNYGQMMAGATITFLPPFIFYLILQSKFVEGVSLSGIKG"
     gene            18391..20139
                     /locus_tag="KR250_001817"
     CDS             18391..20139
                     /locus_tag="KR250_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HBH0067753.1"
                     /translation="MEISYVLKDCTFKHNKYSVVEAETWKDKDLDIVVAKGEKFAFQI
                     MLKATEEFNCTIDKSSAISWKGLGNRVRLALNMPNSLENNFSINILGYVQDDTKAFVN
                     DAILRDKDVLVEQYLPQTFWIEGQVPEDFEENNLDIGIDIFKSFGYEDEEKVCTIDVP
                     VKVRNVVLKPLDESKFFLDLWQHPSCLARMYKVELWSDIHFEIVDNYLKELASLGEKV
                     ATVIVSDYPWAGQSCYKVYKNPSNLYEYNMVSVSKGLDGKIKCNFESMDRYISIADKY
                     KMAKEIDLFGLLGNWCAGEFGNPVEGYKDPIRVRYFDEKDKVFKFINNTNDLKEYIGL
                     VLNHLIECGLWDRVRIIADEPNNPEVVKECIEFINSTVSTHQVKYKSATHDQNFLDRA
                     KDEIDDMSINLKLTIQNYKDIESLKKKINDKGGILTWFVCCFPEKPNSFLSSPFVENR
                     IIGWYTYYFGLDGFLRWDYNLWTEDPWKDSSYKFPIWKAGDMFFVYPGKDLKPVRSVR
                     MENLRFGIQDFELFTMLEKEKGREYIEVELMQELLNKKENAEIKGFGDIELGYSLDNW
                     GYDKVKKYVLELLDRV"
     gene            20401..21423
                     /locus_tag="KR250_001818"
     CDS             20401..21423
                     /locus_tag="KR250_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 5 protein"
                     /protein_id="HBH0067754.1"
                     /translation="MFGGINIKRIGIIIMFFLSISFIGFYLVIRDNSDTVKMQRGINI
                     GNALESPKDFPWDVKMSNKFFDDIKDAGFDTVRIPVRFSDYTSDSDNFKIDEEFFKKI
                     DKYVDYALDKDLIVVLDLHHFEEIMKEPRVHKEKFLKIWQQIANRYQKYDKKLVFELL
                     NEPKENLYSQLLNEYIEEAIKIIRKTNPKRTIIVGPYNFYQIDYLNELNIPKDSNIVV
                     SFHYYEPNDFAFQGNIYHKGFEHLSNITWEGTNEQMDYLKKRFDTVENWANKNKVKIF
                     LGEFGITKEAPEASRRAWIKAVREEAEKRNFSWAYWELASGFGIYNQIEGTWDRDILS
                     ALIEKR"
     gene            21428..23734
                     /locus_tag="KR250_001819"
     CDS             21428..23734
                     /locus_tag="KR250_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBH0067755.1"
                     /translation="MKKNSKKILITLTIITNVIYILWRIFYTVPKEEGKFALICAIIL
                     LFVEIMGMMEMFVHYYGMSNIEYPEKPIISEELYPHVDVFIATYNESVDLVRKTVNGC
                     IHMQYPDKKKVHIYICDDGNREEMCILAEKMGVNYITRTEREGAKAGNLNNAMQHTNS
                     PLIATFDADMIPMHDFLIATVPYFLKNEQAKKDGEKEEYEKVGFVQTPQSFYNPDLFQ
                     FNLHSEGRIPNEQDYFYRDIQLARNRTNSVIYGGSNTVISREALEEVDGFYTYSITED
                     FATGILIQSKGYRCYAIPEVHASGLSPTDLKSLIKQRERWARGCIQTGRRLNILFRRG
                     LGFWQKISYISSITYWYASIKRFVYIMAPILFSVFNVIVVKCTLLQVLVFWLPMYILS
                     SLSLKIFSQNIRNTRWTNIYETIMFQSLMPAVILETFAISKNKFSVTNKSKLEENRMY
                     KFLQGIPYFIYMVLSIIGILKMFVAIFKMSSMTYSVVLFWLIGNLFNLVMATLFISGR
                     QQLRKSERYIAEIDFKLKQNSYVLSSKTIDISENGFAFLLENPEYISPEEEFEVEFRE
                     KSGNEMYIANMKAKIVNVVEVNSKWKYAAYITHIEDSEIDNWMCIVHDRIPTLPMTIS
                     NQLGFFDDLQINVKKRIEKTRTLSRRSPRINMNFQMDIKNTGKLRIVNFNYQYVLLNF
                     ENKSIYPKEIALEINENIVLECDLCEGKIDERGILYKVNNIDSIMQNFFLRDEMMDWI
                     LQNKKILDSKPSEKKEKSIDEFEPMEYI"
     gene            23706..24821
                     /locus_tag="KR250_001820"
     CDS             23706..24821
                     /locus_tag="KR250_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0067756.1"
                     /translation="MSLNPWNIFRRDEMKTLKVFFCSTKYLILVFIFFINTTFISSAT
                     SDKILDLSFKKIETDLSSKITYEDTGVKIETDSSKSDKERYLYIYQNIKENWSMYNNF
                     YIEIQNKNKSSQRINLSIQSKNMLEFRLKEGSEVFLEGKNIIYSDKIKEGCIEVPGEF
                     EGKIYVNFNSLINEESNVVLDSNMLSNIVSWGITFIPSDEEHNIVIIKKISLLSEEKL
                     RFLNNIKIIGDEEVQIPVLGQSISQYEVLGLKSDSKIKYSLMGKQDNVSISQNGKLTL
                     SNKAKPGQIILQVNVDDKFKIGKKITLTESWSINKKDKDGVPYTLVSPEQSPTVQDMK
                     KINFMNNIITFVRILFVSLVIICFGIYLYWKKCSKTK"
     gene            24839..26257
                     /locus_tag="KR250_001821"
     CDS             24839..26257
                     /locus_tag="KR250_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HBH0067757.1"
                     /translation="MLFSSLIFLFYFLPITLVLYYVFRFNRTIQNMILLAVSLFFYAW
                     GEPKFVVIMIVSIIMNYIFGLLVDRYRESKIKVKVFLVLMCAYNIGVLFIFKYLAFAL
                     RNISELISTELTIPNIILPIGISFFTFQGMSYVIDVYRRHGEVQKNPFYVGLYIAFFP
                     QLIAGPIVRYESVAEQILNRKETWNKFSIGTCRFITGLGKKVLISNNMAIVADYIYTM
                     NSQGEIAASLAWLGSIAYTLQIFFDFSAYSDMAIGLGLMFGFKFEENFNYPYISKSIS
                     EFWRRWHISLGMWFKSYIYFPLGGSRVANKDIMIRNMFIVWLFTGIWHGAEWTFVIWG
                     ILNFVFLIIERFIMFEKIENHNFIKHIYALLVVNFGWVLFRSPNLKEAYNYFKAMFGG
                     NGAIWSDYTYMFLKEYWVFFIFAFIFSVPIAKKINKFVVEQAKYSMIFNVFYPISAIL
                     LFFISVTYLVTGSYNPFIYFNF"
     gene            26272..27480
                     /locus_tag="KR250_001822"
     CDS             26272..27480
                     /locus_tag="KR250_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0067758.1"
                     /translation="MNRFFKVRLITAIMFLIILFAFSIFNVCYSGKDTIQEIKNSIAE
                     KSSLKDTISSIDNTVNDKVIYKNTFIETYGYLQKLMGKNEFSKFSVVKDTSGKMHYTY
                     FAKAPNPVGVIADRVRKFSDEMEKQNTKLVYLMTPDKYIKGVTKFPKGIPYSYSNETA
                     DNFLNQLKKYNVDYIDFRENILKSGIPKDDLFFKTDHHWKVETSFWAFGELVEQLNKK
                     YNMNLDENHYYRDKDNYNSIVYPKSFLGSMGRKTGILYGGIDDFTLIYPKFKTNYTYY
                     TDSKSQKFELKGRFEESLILSYPFNADLDLMDGQSDKYFTYLLGNRPLVKIKNIENPD
                     GLKVLFVKDSLIVPTAAFFSSVCSSIDMIDPRYYDGDILEYAKSHDYDFVFISVYPQN
                     LTKEFFPFCE"
     gene            complement(27554..28942)
                     /locus_tag="KR250_001823"
     CDS             complement(27554..28942)
                     /locus_tag="KR250_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HBH0067759.1"
                     /translation="MESSFLSQFLMITDFKTIFFIILLLGTFFIVRQFEKKKIKFSLR
                     TIYATVLGLILGIIIQAFAGFPNDTTQVTWLQEVSKWYSLFGSGFMDLLKMLVVPLVF
                     LSIIKVIINMKDNNLGTLTFKSLGMLLATTAIAAIVGIIVANIMKLGVGANMPTLVEN
                     QELKEINSLVDTLRGLLPSNPVMAMANGNIVATIIFATFIATSIKRLSKKHFETIKPF
                     IDFIEASYKIIVSVSMTVIKFMPYAVVALLANTITSQGISSIVSVLQFILALYISVAI
                     LFIIHLIFITLNGLNPITYIKNSTEALLLAFTSRSSLGTLPVTIESLVQNHGVDEGVS
                     SFTASLGANMGMNGCAGIYPALMAVTLANMAGVNMDMSFYVMLLIVITISSFGIAGLP
                     GTATMAVSVVISGVGLGSYFPLVGGILAIDPILDMGRTMINVNGAMVSSITVGKSLGK
                     IDKEVFSKKNIS"
     gene            29283..30617
                     /locus_tag="KR250_001824"
     CDS             29283..30617
                     /locus_tag="KR250_001824"
                     /EC_number="1.8.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HBH0067760.1"
                     /translation="MRVIIIGGVAAGMSAAAKLKRIKPEYEVVVYEKTEIVSFGACGL
                     PYFVGGFFNDADELLARTPEKLREAGIDLNIFREVVEVDSESKKIKVKNIKTDEIYED
                     YYDKLMIATGARSIMPPIKNIKLKNVSTLKSLYDGEYLKKLLSNEDNKRVTIIGAGFI
                     GLEAVEACKKLGKDVDVIQLEDRILPQVFDKEMTDVLEEEINRHNVNLHLDEMVVELC
                     GEDKVEKVITNKGEIDTDVVIIATGVRPNTEFLSNTNIKMLKNGAIVVDEYGRTSVED
                     IYSAGDCATIKNIVSNENVYVPLATGANKLGRIVGENLAGREVSYQGSLSSSCIKIMD
                     MEAASTGITEKQAKDLGINVKSKFISDYNQTHYYPGRNKIYVKLIYDADTKVILGGQV
                     AGFKDAVQRANVLAAAIFGKMTTNQLGMLDLCYAPPFARTWDVLNVAGNVSK"
     gene            complement(30759..31676)
                     /locus_tag="KR250_001825"
     CDS             complement(30759..31676)
                     /locus_tag="KR250_001825"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011969064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HBH0067761.1"
                     /translation="MIDLTLLGCGGNVPMPNRFLSSVFINYKGRKILIDCGEGTQVSM
                     KLKKCGFKDIDLICITHLHGDHIFGLLGLLSTIGNSGRTSDLTIVGPVGIVDCIRSMR
                     NLVEYVPYTLKIIENPQGNFSLDNKVLRNLEISTISLEHSIECLGYSFNFRRNPKFDI
                     DKATKNEVPKILWNKLQEGQNIVLDSKQYTPDMVLGELRKGVKISLTTDTRPIESIPD
                     FIKDSDLFICEAMYGDDLDISKAVRNKHMTFREAANLAKLGNVKQLLLTHFSPSLDIP
                     SMYLENATNVFENTILGEDRLSLRLNFDE"
     gene            31884..32768
                     /locus_tag="KR250_001826"
     CDS             31884..32768
                     /locus_tag="KR250_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4846 domain-containing protein"
                     /protein_id="HBH0067762.1"
                     /translation="MKVLKKITILSLITLTLVGCSNGEDKKVVEGSKKIQNSKKDEQP
                     IIVHKDANILKDRYEPPKGFKRVDVEKGSFESFLRNTKLKNYGEKVKYYDGRTKESKG
                     IYDSVFDVDIGNRDLHQCADAIMLMRAEYLYQNKLYDEISFDFVDGFKAKYSKWAQGY
                     RISVKDSGSSWYKATEYSTSYESFRKFMDIVFAYSGTLSLEKELEPVKVEDMKIGDVF
                     IVGGSPGHASIIMDMAENEKTGEKVFMIAQSYMPAQQTQLLINRKNPNLSPWYSLDFE
                     GDLTIPQWTFKRDQLKRF"
     gene            32981..34822
                     /locus_tag="KR250_001827"
     CDS             32981..34822
                     /locus_tag="KR250_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HBH0067763.1"
                     /translation="MKIRRYLILVIIILITTSQSFISYGLERFEEITIFHTNDIHGRY
                     AEGDDHIQIGNLATLKKETPNSILVDAGDCLHGLPIVNMDRGKSAIELIKCAGYDYIT
                     PGNHDFNYGKDRLFELSKSANLGENTLKFLSSNIFENGKKVFDSNDIKEIDGIKIGFF
                     GLSTQETKSKTGFKNVEGLEFKNPIESATEQVNELKGKGANVIVAICHIGTNSLSKPN
                     SIDIANEVQGIDLIIDGHSHTKFENGKQVGDTLIVSAGQYLENIGEIKLTLDTNDVEN
                     IKIENKSVRLIGKEEALKYKLDSAVSKKVDEIKEQQEKILDKVIGVTTNTLDGSYENV
                     RTKETNLGNLISDILLDKTKADISLFNGGNIRDTIEKGNITRRDIVDVFPFSNTIVTK
                     ELTGAQIKDVLEHGVKLYPEKSSAFLQVGGISYYFNPKQKEGEKITNIQKEGKPLDLN
                     KKYVVATNDYIASGGDEFPCFSKEPVLKEFGNLESAVIEYIEYKKEISKNVDGRIGIK
                     SKDELISKNHESDLILSENEKNINDNEENLSNNIKSSDSDKNKSLDKTSARESSNLGI
                     SKREAKEKSPKTGDLGFSNSIIIFIVSSTLICLLNFNQKELKDKKSK"
     gene            35127..35834
                     /locus_tag="KR250_001828"
     CDS             35127..35834
                     /locus_tag="KR250_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0067764.1"
                     /translation="MNNILLLEDDKSLNRGISFKLKKEGYNVFSAFGIEEAKNIFAKE
                     EICLIISDIGLPDGSGFDFCEEIRKKSDVYIIMLTALDEEVDIVTGYDLGADDYITKP
                     FSLMVLISKVNALMKRVNTVKNYTLLVCDDLFFYYIENKLIIRTDDKEEEIILSKTET
                     KLLKYLMENSMQTLTKEQLLESLWDSSGNFVDDNTIAVNIRRLRQKVEKNPSEPKYIK
                     TVRGVGYMWGERSIKKC"
     gene            35828..37138
                     /locus_tag="KR250_001829"
     CDS             35828..37138
                     /locus_tag="KR250_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBH0067765.1"
                     /translation="MLKNMNLKDGLSVKDDYIMRRVIVYSVLSVLIVTLVFILFEYSR
                     LHKLYEHQIKNTQNIAGILIEKYPNDEIDIVNSIYNSDYSKVSTGEKAFKKFGYGLEN
                     KMSNDKNFSLYLKSFFKESFFIFLMMFIVVIGIIYYFIRYINKRLSKIYFIVDKMSQE
                     NYLKDTTESEETGYLVEYNEYFKEGIFSKINNCLYELNRSLKIKFIKLEKEKESVKSL
                     VTDISHQLKTPLASLKLYNTLLIEEELEEDEANEFLLTNKASINKLENLINSLVNISR
                     LEISMINIKKEDNDIKNTILNAVKSVTPKAKSKSITLNLNEFNSRIIPHDKKWTEESI
                     FNVLDNAVKYTQVNGEINISVEETANFFKIIIEDNGIGIEKNEFNNIFKRFYRGSADE
                     IESVEGSGVGLYLSRKILEEQGGNIIVSSKVGIGSKFSLFLTTM"
     gene            37255..37938
                     /locus_tag="KR250_001830"
     CDS             37255..37938
                     /locus_tag="KR250_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0067766.1"
                     /translation="MKILYTENLSKHYGKGESLVRALDNVDLEINEGEFVAIIGKSGS
                     GKSTLLHMIGGLDIPTSGKVYIDNKNIFTLKEEELAVFRRRKIGFIFQSYNLIPSLNV
                     WENVVLPIGLDGREVDESFIKELLKSLGLENKHDVLPNTLSGGQQQRVAIARALATRP
                     AIILADEPTGNLDSKTSDEVMSILKSMSKKYSQTLVMITHDDSIAQMADRVIFIEDGR
                     VSKVGDKND"
     gene            37931..>38753
                     /locus_tag="KR250_001831"
     CDS             37931..>38753
                     /locus_tag="KR250_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0067767.1"
                     /translation="MINTTKIAKSNLKQNKSKSILIIITIILSTLMLSSIGIYIVDAG
                     AYQKENTIKYSGNYQGILANVDEKQADILSNHADVELTGEMNGVGVEKLEDDSNISLA
                     YMNEDALKLNSFEFIKGKLPSKENEIVLDSGALKALGYKNKDLGEKIKISYDDYKNDK
                     KIEKEFIISGILKTSEISEAGKYYYAIISESYMRNTRDMSQEDFNIYVKFNDKSNLSI
                     EQAKEKLDKIANDIGLDTINTAVNENYINALKPDMETIMGGVFVGLVIVLSSILVI"
BASE COUNT        15306 a         4114 c         6800 g        12533 t
ORIGIN      
        1 tactatagca tcccttaaaa acttagctaa tccaggttgt tctctatcat aatatgatgt
       61 aaaacgttca tcatatacat acatctgtgc tagagaggaa tgagcttctt tagagtatgt
      121 gttccaagtg taactcaacc atttatgatg taattttgca acttcctgag ccagttcact
      181 agaggcatca ccagttttaa aagctttttt aagtttacta attatttcga tatttaggtt
      241 ttgccaattt tcataatctt gcttagacat atttttaagt tttttatttg atgtatctat
      301 aatatcttta ccatatttct ctctaatttc agttccataa tttttttcat tttcatcaat
      361 catcttttct ttaaaacctt caaatttttc tatatctgtc atagtgtatt ttccctcctt
      421 cagagctata gttttagtta cattttctat tagtaaatca atttgctttc ttttggctag
      481 taatttatta tggtgttctt ttaacgcact taaactatcg aagtttggtg aatttataat
      541 gttttttata ttttctaagt taattcctaa ttctctataa aaaagtattt gttgaagttt
      601 attaacttca ttttgaccat atatacgata acctgaagaa tttattttaa gtggtttaag
      661 aagacctatt tcatcataat atctaagtgt tctagtactt ataccagcta gtttacttaa
      721 tttttgaact gtatattcca taagtagtac ctcctataaa ttaaatatac acttttacgc
      781 aacgtaaatg tcaatacatt aaaatataaa aatttatatc ttttaataaa aatataaaaa
      841 aatatcttta ctggatattt tacacacaac tgtcaatact cactaatgaa aattaaaaaa
      901 taaaatttat agactatctt tactaaaaaa tagagggtct atagtctagg aggaactgta
      961 tgaatttaaa aagaagcaat gaaaaacgag aagagtacag aagaatgtat tcagataaag
     1021 aaagtttttt aagtttgatt caaaatttta caagtgtctc aatagcaaaa gaattaactc
     1081 ttaaaaatat agagttagaa acatctttta tatgtgaata taaaggaaaa gaagttgata
     1141 taatttataa ggtattttct aaaagtggca aagtatcaca ttatatagtg ttagaatttc
     1201 aaacagaaat ggatactgaa attgtcccaa gattgaaatc gtatagagaa caaatttgga
     1261 aaagttttat tatgaaaaaa agccttgaag aaatagaaga taaaaatttt aaacttccta
     1321 aagttatacc agttgtttta tatagtggac ctgaaaggtt aagtagaaaa aaaggaattt
     1381 tggatattgt agaatctata tcggagttca gtaataattc tagtatgaaa aattaaaatt
     1441 acataatgtg tttattggtc taaaatggaa aactgtcgca taaacttgaa tatgaaaata
     1501 tttatagata caggttctat gtggaggtgt tccatgagaa ataacaagtc taaggtattg
     1561 ctaggaatac tttgtctagc aacaggaatg gctgtaattc tgtcaatgtt gcttcctggc
     1621 tggatttggt cagcacttac cgcattaata cttattggat gtggagcatt attatttttt
     1681 tgttaaaata gttttaacta taaagtgtgg ggtgagtatt gatgaaagta gtaacaataa
     1741 aattaccaaa atggatttca agtataatcc taaaatttat gagaaagagt aaaaatgatt
     1801 aaattaaaaa gcctacttac ggaagtaggc ttttaaacgt ggttactaag ctctttcaat
     1861 tttaccagaa cgtaaacatc tagtacaaac ttttactctc ttaggagctc catctataat
     1921 cgctcttacg cttcttaagt tagctgacca tgttctttta ttatgtttat ttgagtgtga
     1981 tacttggttt ccagaaacct tacctttacc acatacgcta catacttttg ccatctacgc
     2041 acacctcctt aaaatgataa ctatttaaag actaaaaaca tatttatatt atcataaata
     2101 acaaaaattt gcaatagaaa tatatatttg ccttacacat attgaagaat ttcttattat
     2161 catataaaat tatatatata attttatata aagtaaatat atttggaaaa taattattat
     2221 ttttctttga atatatatac tatagaagaa attttatcca aaaacttttc taggtttaaa
     2281 ttataccata tttatagtat gatatgatta taattttata taataaataa gtaaaaatag
     2341 taaaaaataa attaagaaat gtttttaata aaaataagct taagggggtc aaccatgagt
     2401 gcaaaggtaa cgaatcaata tggaaatata gaaatagata atcaagtaat agctcaagtc
     2461 gtatatagag ctgcaatgga aagttatggc ttagtaggtt tagcatataa gtctaaagga
     2521 atagtagagc tattaaaagg cgagaacgca actaaaggtg taagagttga ggaagtagaa
     2581 gaagatgcca tagcaatcga actttatgtt ataatacaat atggtacaaa tatatctact
     2641 gttgccaata atataataga tagagtaaaa tatgtagttg aaaaaatgac tggcgtaaga
     2701 gttactagaa ttgatattaa tgtacaagga ataagagtta agtaatttca taggaggaaa
     2761 caaatgattc aatatataga tggtaaaagg ttaagggaga tgtttatttc aggtgcaaac
     2821 aatctccaaa acaataaaga gttagttgac aagttaaatg tattccctgt accagatgga
     2881 gatacaggaa ctaatatgtc attaacaata tcttatgcct taaaagaatt agcaaaagtt
     2941 gaaaatgata atatatcaga tatcggaaaa gcattatcta aaggttcttt aatgggagct
     3001 agaggaaact caggagttat actttcacaa ataatcagag gtatagctaa atctatagaa
     3061 ggaaaaagta aattatcaac agaagattta gcaaaagctt ttaaaaatgg ttcagacaca
     3121 gcatataaag ctgtaataaa accaattgaa ggaactatac ttactgtagt tagagaaagt
     3181 ggagagtttg caataaaaac tgcgaaaaaa gagaaagatg tagtaaagtt tttaagtatg
     3241 cttgtgaagg aatcaaattc ttcattagaa agaactccag accttctaaa gaatctaaaa
     3301 gaagctggtg tggttgactc tggtggaaag ggtcttgtac ttatatatga aggaatgtta
     3361 gcttctataa aaggcgataa catagaaata aaaaatgttg acctagatac taatatttca
     3421 acaagtatgg attttgcaaa gagtactact agcacagatg atataaaata ttgctattgt
     3481 acagaattta ttttagaaag ttctaaggta gaagatacta aaataagaga cataatgatg
     3541 gcttatggtg atagcctagc agtagttgga gatgatggag tcataaaggt acatgtacac
     3601 actaatgacc caggaaatgt acttcaagaa gctctaaagt acggtcaatt attaactata
     3661 aaaatagaaa atatgaagtt acaacatgaa aatacattac tggatgtaga agaaaagaaa
     3721 gaaaatgata gtgaaccttt agaagaagaa aaagagtttg ggtttatagc tacttctatg
     3781 ggtgaaggtt tggcaaatat atttaaagat ttcggagttg accatataat agaaggtggt
     3841 caaactatga atccaagtac tgaagatttt atgaatgcta taaagcatat aaatgctaaa
     3901 aatatattta ttttcccaaa taacagcaat ataataatgg ctgctaatca ggcaaaagaa
     3961 ttgagtgata aaaatataat agttatacct acaaaaaata cgcctcaagg atttgctgca
     4021 ttagtaactt ttaatggaga attaagcgaa gatgaaaata aagaagctat gatgaatgct
     4081 ttaaactcag ttaaatcagg tcaagtaact tttgctgtta gggatacagt gatgaacgaa
     4141 attgatgtta aagaaggtaa tataattgga attgcagaag ggaatttatt atcagcagga
     4201 gattatgtgg atgaagtaac atcaaacctt attgaaaagt tggttgatga agatactgct
     4261 ataataacat tattctttgg tgaagatgtt acagaatctc aagcaaatga actacgtact
     4321 tctttagaag aaaagtttga agatgtagat gtagagttat attatggagg acaacctctt
     4381 tattattact taatttcagt tgaataatga ttataagaac ctgtatgacc aagtagcttt
     4441 tataaaattg gttgtacagg atttttgtgt agtaggtgat atatttgttg gatttatata
     4501 aagatgttca atatgttaag ggaataggac ctaaaaaagc agataaatta aataaactgg
     4561 gtatatttac actaaaagac ctattgtatt attttccaag acaatttgaa gatagaaata
     4621 atctaaagaa aatagcacaa ttagaagatg gtgagaaagt tacgataaaa gcagtaatat
     4681 caagtataaa tacatttagc ccaaaagaag gtatgacttt aacaaagatt gatgtaaaag
     4741 atgagacagg ctcggctaaa ttagtgtttt ttaataagag ttatataaaa aatacttttc
     4801 gtccaggaga ttcaatatta gtttttggga aggttaagaa aaaatttaat aacttagaac
     4861 ttacatcatg tgaattagaa tatcttacta attcacctaa gaatacatgt agatttatgc
     4921 ctgtgtacca gcttacatac ggtgttacaa ataaagaaat tatgagtata attagaacag
     4981 tacttgaaga taaagaatta attatacaag aatatatgcc acaaagaatt atagaaaaat
     5041 ataggctatg tagtatagat tttgcagtta gaaacataca ttcaccaagt agtaaggaat
     5101 cattaaaaat agctttatat agaattgtat ttgaagaatt acttatacta caactagggt
     5161 tgtttgtatt taaaagtggc agaaataaag aagatggaat aaagtttgaa acaagtaaag
     5221 acttaaaaaa aataataagt gctttaccat ttaaactgac taaagctcaa aacagggcat
     5281 tagatgaaat tatacaagat atgaatttag agaaaataat gaatagattg gtacaaggtg
     5341 atgtagggag tggtaaaaca gtagtagctc tcttagcact tgcaaattgt gttttaaatg
     5401 gttatcaggg cgctttgatg gcacctacag agatattagc agggcaacat tatatttcac
     5461 ttactgagtc tctaaaagat tttggaataa atgtaggtct attaataggt agtcttacaa
     5521 aaaaacaaaa ggatacagta ttggaacaaa taaagaataa tgagattgat atattaatag
     5581 gtacacatgc actaattgaa gataaggttg aatttaataa tattggttta gttataacag
     5641 atgaacaaca tagatttggt gttatgcaaa ggagcaaact ttcattgaaa ggagcaaatc
     5701 ctgatatatt agtaatgaca gcaacaccaa taccaagaac tttagcactt atactctatg
     5761 gagatttaga tatttctatt atagatgaac ttcctccagg aagacagcca atagaaacaa
     5821 ttgctattga aaaaagtaag cgagatagag catataataa ccttgtaaga agagaggttg
     5881 aatcaggaag acaagtgtac attgtatgtc ccttggtaga agaaagtgaa gccatagaag
     5941 ccaaatcagc agttgaactt gttgaggagt taagagcgga atattttcac gacttaaggc
     6001 ttgggttgct tcatggtaaa atgaagtcaa gtgaaaaaga tgaagtaatg agacttttta
     6061 aagataaaga aatagatatt ttagtttcaa caacagtaat agaagttggt gtcaatgtgc
     6121 caaatgcaac attaatgata atagaaaatg cagaaagatt tggacttgca caattacatc
     6181 agcttagagg gcgagttgga agaggtagtc ataaatcgta ttgtgtacta atttatgatt
     6241 ctaaaacaga tgtatgtaga caaagaatgg ctataatgga agagaccaat gatggtttta
     6301 agatttcaga gaaggattta gaaataagag gacctgggga attttttggg acaaggcagc
     6361 atggtcttcc agaacttaag gtagcaaact tatttaagca tataaaaata ctgaagttag
     6421 cacagcaaga agctcgatat atattgggtg aagataataa tctacaatta aaagaaaaca
     6481 tggcactcaa aaaagaaatt atagataaat ttaaagatac attgaaagaa atttcattaa
     6541 attaatttta aattgtgtta aaatattact aatgtaataa tgtgctgaag ggagattgtc
     6601 atattgagag taatttcagg aaaagcgaga ggattaaaac taaatactcc aaaaaatgaa
     6661 gatgtaaggc caactacaga tagagtaaaa gagtctctat ttaatatgat aaattcatat
     6721 ataatggaaa gtgaagttct ggatttattt gctgggactg gttctttagg aatagagtgc
     6781 ttatctaggg gtgccaagtc atgtactttt gtagatatca gtaaggaaag catagatatt
     6841 gttaagtcca atataaaaaa ggcaagagtt gaaagtgaaa gtattatttt aaaccttgat
     6901 tttaagactg caatagataa acttaaatta caaaatagta aatttgacat aatttttatg
     6961 gaccctccgt attataaaaa tatgtttata gaagctattg aaaaaattga taattctaat
     7021 ctattgaacg aagatggaat aatagttgta gaacatgata cgaatgattt atttccagat
     7081 aagatatgta aattggagaa aactagagat aagaaatatg gaaatactac attaactttt
     7141 tataagatgg aggcataata tggaaaataa acctagaaaa gctatatttg caggaagttt
     7201 tgacccaatt actaatggac atttagatat tatttgcagg gcttcgaagt tatttgacga
     7261 attacaaata ggagtattaa ataatcctaa taaaaagggt ctatttagct ttgatgaaag
     7321 agtaaaactt atagaaaaaa gtacaagcca tttaaataat ataaaagttg tgacttttga
     7381 tggtttatta ataaattact gtcaggaaaa tgggattgga gcattagtta gaggtgtgag
     7441 aagtggtgct gatgttgatt atgaacttca gatggcgcat atgaatagag agcttaatcc
     7501 agatatagag actataattt taccaagttg tacaaaatat tcatttataa gttcatcttt
     7561 aataaaagag gttttgctct ttgatgctga tataaaaaat ttggttccaa agattgtttt
     7621 ggaagaatta aagaaaaaaa ctagtggggg taattgatat atgaagatag atttagaaat
     7681 gattgaacta tttgagcagt tagaggagat atttagaagt gcttctacaa tccctttctc
     7741 acataaatgc acagtggata aagatgaggt tttagcaata attaatgata taagaacttt
     7801 aataccagaa gaagtaacac aggcagtatg gattaataaa gaaagaaata aaataataag
     7861 tcaagctaag caagatgcta caaatatagt tgaacaagcg aaaaaagaag ctgagagaat
     7921 acaacaagaa tatcaagata atatagagga attaaagaaa aattctgaag atgtagtaaa
     7981 ggcatatgta gaatccagtg aacctgttgt tcaagcagac caaagagcaa aagatatagt
     8041 tgatagagct gagagaatag ccaatgagat aagaattggg tcaattgaat atgcagagga
     8101 tgtgctttct agtgttgagt ataatttaag agaaatatta gaagaagtaa aaagagataa
     8161 agctgaactt agacctaaaa aataaatttt aaataaataa aaatggagct atcacacaaa
     8221 aaattccaat ttatgtgtga tagctctctt ttaatatagc aattttttgt tatataattg
     8281 tgctttttag attacttata tcttattttg atattataat gtagtttaat cttttatttg
     8341 ttgaaatcaa cttatttatt tgtataaata atataggtta ttttcaaatt tttttaataa
     8401 ttttaccact gtcttctttt agattagagt tagactgtgc aaaattataa aataatacta
     8461 aatcatgtgc aattatttgg tatggcacta catgtagaaa atatagataa taaaattaag
     8521 gaggtagtat gaaggaaagg gctaaaaaaa tattagattt tattattaaa aataacaatg
     8581 taacatcaca agaactacaa gaaaagttta atgtttcaaa gagaactatt tattacgata
     8641 ttctagctat aaatgaacaa ctaggaaaaa gtggtaatat aaaaaatgta aaacacaaat
     8701 ttatatttga aggtaattta tgtgatgcaa ggaaaataat ttcaactgaa gaagataaat
     8761 ttttagatag tgattacaga aaaactttta tattaaacaa gatattactt ggcgaaaaaa
     8821 tatcaataga aaaactaaca aatgaaatgt tactttcaaa aaacacagta gtacaaacaa
     8881 taactgatgt aaaaaaatac cttcaaacaa tggggttaag attagagtat aagggaaagt
     8941 acaaaattat aggtgatgaa tacgttatta gagagctgtt tctgataatt gttcaagaaa
     9001 atgtactgga aattaattct ataagtgagg aagtaagtag tttcgataca aaaggacata
     9061 taaaacttac tgattattct ttattaaatc taactaagtt tgtggaattt ctaaataaaa
     9121 ggataagaga tgggaaaaca ttatactcat ataaatatct aaatgaagct aaaaaaatta
     9181 gttactttag taattgtaaa gaacttttat gtgaagaagc taatgaaaat gaacaagcct
     9241 atatatgcac ttacataagt tcacttccca gcttaaatag tgaagttaag gaagatgttg
     9301 ttgaagaata tgtagataaa cttatagata agtttgaagt aaatacagca ataaaattag
     9361 aaagtaagca tgagtttaag aaaaatattt taaggcacct ccactcttct tacaacagaa
     9421 tacgatttaa gtttcctatt agaaatccaa tgttagatga gactaagtat aaacatgagt
     9481 cactttataa aattataaaa tcaattatag agaatgaaga agagtttcct gtatttgagg
     9541 ggataagaga agaagaaata ggatttatag cagcgtattt tggaggatat ttaagagggt
     9601 ctagggataa tggtctaaga agaaataaag tcttactagt ttgtcctaat ggtcttatgg
     9661 tatcaaagag tttggaaata caactatata aatacatacc aaccatagaa atagtaggta
     9721 tagtatccat taagcaacta aaagaagtaa atgtatacta cgattacata attacaacta
     9781 tagatataca aaatgtaaac aacgtaattg ttgtaaatcc tcttttaaca tcatcagatg
     9841 ttcaattact tatgaacaaa ctaataagtg taaaagaaaa cgagaagtat ttcaatcttg
     9901 aattaataat acaagctata agaaaaaatg gagttatcaa taatgaagaa gcactaaaag
     9961 ctgatttatt aaacataata cacaagatag atgaaggaga gatgtatcaa ccaatgttaa
    10021 aggaattaat taatgcagaa agggtcaata taataaaaaa tgttagagat tggaaagaag
    10081 caataaaaat agcctcaaag ccattgttag aagataactc tatagaagag ttatatatag
    10141 aaaatatgat aaagtcagtt gagaaatatg gaccatatat tgttttagct gatagatttg
    10201 ctctaccaca tgcaagttct aaagaaggag taaacaagct tgctatgtct ttactaatag
    10261 tagaagatga agttgattta ttaggaaaac cagtaaatat atttatggta ttagctgctg
    10321 tagataacac tactcacatt agagcattag catcactatc agagatgatg tacgaggaag
    10381 aaaatgtaaa actaataatt aatggtgata aaagttcaat aatagaactt ataaataagc
    10441 aaaattaggg ggaatgaaaa atgaagatat tagcagtatg tggttttggt gtaggttcat
    10501 caatggtatt aaaaatgaca atccaaaagg ctctaaagca gttaggtata gaatgtgaag
    10561 tagaaaatac agatataaat tcagctagag gtgctgattg tgactgtata ttcacaagtc
    10621 aagaattgtt aaatgaatta aaaggaactt gtaatgtaga tgtatatcca gttaaaaagt
    10681 acatggattt agcagaagtc acagaagttg ttcaaacttt tttaagtgat aaaaatgata
    10741 agaaatagta taagttttaa aaagtaaatt taagttaatc agaataaaat aaaaacttac
    10801 taagttcaaa aatagtaaga aagagaggag ggatttagtg gtagaaaact taaacagttg
    10861 acctatcact aaaaaatata atgcttgatt aaaaaatagc tacgcacact cgtgacctta
    10921 gtcatgagtt agtagctaaa atagtcagca tatagggaaa cctatatgta gagataggat
    10981 agaacctatc caatactact tgaattgctg gaaaccccta aagctaacca aactaaaaca
    11041 taaggatgaa ataaacctaa gtgtgaaagt tacgaaagta gaaaaaattg gttagatggt
    11101 ataaggttaa accctaagta ctttaaaatg ggcaatcagc aggtaagctc cgaataggag
    11161 aaacttcaac gactattcct cttgagggaa gtacactaca agctattggt agtggaagtg
    11221 ggtagacctt aacggataat gccgaaggat aagatatagt ctgtgcttac gtgaaagtgt
    11281 aagaagttca taagagaact gcataggtag tagcgaactt atgtgaacga caacctcaaa
    11341 aatgataaaa gttaagaact tacggttctt atatatgtta tttaaaaata aatataaaaa
    11401 accttcaaaa tacttgtttt aagtaattgc atattttaat attatgataa gtgatgaaaa
    11461 atggaagtta ctcatggtag aggatatgca tatagtatgt caaatataga catccattat
    11521 tatggggaga catctttgga atcctagtta tttcgttgct actgtattag aaaatacaga
    11581 ggagcagata ggaaaatata ttaataatca gaaaaaacaa gtaaagtgag gtgatagtag
    11641 tggaaaaagc ttataagttt agaatgtatc caaataaaaa acagcaagag ttaattaaca
    11701 aaacttttgg atgttgtaga tttgtatata ataagtatct tgctaaaaga atagaagttt
    11761 ataaaaacga taaagaaact tttacttata aacagtgtag ttctgatttg accaatttaa
    11821 aaaaagaatt aaagtggctt aaagaacctg acaaattttc actacaaaac gtcttaaaag
    11881 atttagataa tgcatataaa aaattcttta aagaaaaagc aggatttcct aaatttaaat
    11941 caaagaaaat taatagattt tcatataaaa ctaactttac aaatggaaac attatgtatt
    12001 gtggtcaaca tataaaacta cctaaacttg gtatggttaa gataagggat aaacaagtac
    12061 ctcaaggtag aatacttaat gctgctatat ctaaagaacc aagtggtaga tattatgtat
    12121 cattatgctg tactgatgta gatattgaag catttgaaaa tactaataat cagataggtt
    12181 tagatttagg aattaaagaa ttttgtatct caagttgtgg agaatttata gaaaatccta
    12241 agtatcttaa gaagtcatta aacaagcttg ctaaattaca aagagaatta tcgagaaaaa
    12301 caatcggtag tttaaataga aataaggcaa gattaaaagt tgcaagactt caagaacata
    12361 tagctaatca aagaaatgat tttctacaaa aactatctac taaattaata aaagagaatg
    12421 atattatttg catagaagat ttacaagtaa agaatatgat tagaaatagt aaactatctc
    12481 gcttaatttc agatgtatca tggtcagaat ttattcgtca attagaatac aaggctaatt
    12541 ggtatggtag acaaattgta aaggtaggta aattctttgc tagttcacaa atatgtaata
    12601 aatgtgggta taaaaacgag gaagttaagg acttaaatat aagagaatgg atttgtccta
    12661 gttgtaatga aactcacgat agagatataa atgcaagtat aaatatatta aaagaaggat
    12721 taagactaat aacgattcaa aataaataac atatgtaaga accgtaggaa ctacggggat
    12781 agcctgtgga gaattagtaa gacatatttt agtatgcaaa attctatgaa gcaggaaccc
    12841 tgtgacttta gtcatgggag gttcagttta tttcaaataa tattttacgt aatccatcta
    12901 tgttaattgg attaatagca gcatttggtc ttgtactaca gaagaagaaa gcatctgatg
    12961 ttataaaggg gtcactactt gcagctttcg gaattataat tatggagact ggaacaggta
    13021 tgttagttag ttcaatagca ccaattaatg gagctttcca aagtataagt ggtggtgcag
    13081 taacaaaagg tttaagtgat gttactttta ctgcaagtta tggtggaatt ataggtttag
    13141 caatgtttgc gggtttagct attcacctaa tgatagcaag atttactcct attaagacaa
    13201 tcttcttaac aggtcacatg ctttattggt tcccattcat atttgtagct tcaggagttg
    13261 aagctggatt aaatggtgga gtattaataa cttttgcagc agtattatca gcactctatt
    13321 ggtcaataat gccttggtta ttaagaaaat atgtgtttga tgttatagga gatgattctt
    13381 ttacattagg tcatccatct ggttgtttag cacttatatc aggatttgtt gctaaattag
    13441 taggaaataa agaaaaatct acagaagata taaacatacc tcaaagttta tcatttttta
    13501 gagaaatatc tataagtggt ggaatggcag tgtttttaat gtttatagtt gttgggatag
    13561 taataccagg tgcgtttaaa gatagtaatg agtttattgg aacgtatgca ataaagcaag
    13621 gtctttcttt tggagcaggt cttataataa tgcttcatgg ggtaagaatg ctaataaatc
    13681 aaataatgcc agcatttcaa ggtatatctg aaaaagttgt gccaaatgca ctacctgctt
    13741 ttgactgtcc aatattattt aattataaac caaatgctgt attaatagga ttcttagttg
    13801 ctatgctaac atctacagct atgattttaa tatataacta ctttaatgta tttaatttgc
    13861 tacttatacc acttgttata acttcattct ttgagtgtgg agcagcagca gtaataggag
    13921 aatcacaagg tggtatacgt ggagctataa taggtacagc tatagctgca atatttatgg
    13981 tagttttagt aggaatatct gctgtaattt actcaggaac tattcaaaat tggatactaa
    14041 tatttggagg taatgacctt tcattatttg gtataatcag taggtttata ggttcactat
    14101 ttggaggaat attatagata tgaaatttaa atacatagat gagataagaa gattaattga
    14161 tataatagaa aacgaagaaa aagtcaatat ggaaaaagca gtacaaatct ttgttgaagc
    14221 aatagaaagt aaaaatgcta tattttcatt tggagcttca catgcaggaa tattaacaga
    14281 agaattattc tatagagcag gtggctgtgt tcttataaat ccagtatttg caaaatctct
    14341 aatgttagat acagcgccta taactcacac aagtaagatg gaaagattag aaggatatgg
    14401 tacagctcta gctgataaaa ctccaattaa aaaaggagat gtagttatag ttcattcagt
    14461 atctggtaga aatccagtaa gtatagagtt tgctatgaaa gctaaggaaa ataaggcaat
    14521 agtaatatgt ataactaatt taagttactc aaaaagtgta tcttcaagac attcttcagg
    14581 aaaaaaactt tatgaagtat gcgatttagt gatagataat catggtcaaa agggagatgc
    14641 ctgtgtagag atagatggat taaaccaaaa agttagtcca acttctactg taatagcaac
    14701 tactgtgatg aactctatta tagctcaagc tactcaggaa ttagttaata aagggctaaa
    14761 gaatcctccg attttctata gtgccaatat tgatggtgga gatgagttaa ataagaagat
    14821 ttttgatgaa tataaaagtg taattcatta tgaatattaa ggttaaaaaa atatgttaat
    14881 gaactttgaa gtttatcaac aaagaatatt aataatatat aataaatgct atcatttaaa
    14941 agctgtaggt aaaaatctac agctttttat tattgtagat taaaaagatg cgtaattaaa
    15001 ttttaaattg gagaaaataa attggaaggt atcaattata aatattagaa acgttgtcat
    15061 agaaaaaaac tatattaaaa aaaagtacat agaaaaaact gattagacaa taaatacatg
    15121 ctttgataat attttaaatg gaatgaaaat gtgtagaatt ataatgaaaa aagaaaataa
    15181 aaatgaaaaa agaaaataaa atggagggaa taaaagtggg tatgttaaaa aaattatgtt
    15241 cactagctat ggtagcaatt atgactacaa cacttataac agggtgttca agtggaaatg
    15301 gaaaagattc aaaaaaggat ggcgaaaaag aaaaactagt tctttggatg ccaccagtag
    15361 aaaataatat gaaggaagtt tgggacccta ttttagataa atttgaggaa aaaaataact
    15421 gtgaagtaga tttacagata attccttggg ataattattc tgaaaaattt gcaacagcta
    15481 taagtgctgg cgaaggtcca gatgtaggat atatgtatgc agaaatgtat ccacagttta
    15541 tagaaagtgg agcagtagaa gatttgacta actatgcaac taaagaggat aaagagcaat
    15601 ctatttatat aaaaacatct gaaatgatgg gtggaatgta tggtatgcca ttccaagcag
    15661 caaatccagg ggtattatac tataacaagg atattttaga gaaactagga gaaaaacctc
    15721 ctaaaacatg ggaagatttt aaaagaattt gtaaaaaagc aacgcaagat actgatggtg
    15781 atggaaagat agaccaatgg ggattagctc agggatgggg agctaaaact tttggtaact
    15841 taaactggaa ctggtatcct tatttatggc aagctggtgg agatatattt aatgatgatt
    15901 taaaatcggt taaatttaat gataagagtg gtttagaagc agctaatttc ttaaaagaat
    15961 tacaagcata tgtgcctgaa gattctttat ctaaagatag taatgaaatg atagaaagcg
    16021 tatttggacc agggaaagca gcatttacaa taatgttatc ttctgctgca acaagtgtat
    16081 ttgatgaatc attcccggat ttaaattggg gatttgtaac tggactagaa gataagaaag
    16141 cagcaacttt tggagcagta gaccatttat ctttaatgtc atctgctaaa aataaagaac
    16201 ttgcatacaa gttaatacaa catatgttaa gtgttgaatc aatgacagaa tttcataaag
    16261 caataccaag agcaccaata acaaaaggtg aaccatatca aggtgatgaa agattcaaag
    16321 aaatggtgga aaatgacaaa aatgtatata gaccattagt agtaggacct catggagttg
    16381 aaatatatga atatttatgg aaagaacttc aaactatgat ttcaggagat aaaactccaa
    16441 aacaagcgtt agatgatgct gcaaaatatt caaatgattt attagcacaa taaaagttta
    16501 aagctgtaat atgttgtaaa ggactttaca gattaaaatt agcattgtat ttataaatac
    16561 aatgctaata tttatattgg agaggataaa atggaaaact tagttagaga gtttgaagaa
    16621 aattcaaaaa gaaaaaaaac aaaattaaat attaaaccat atatgtatat attgccattg
    16681 ggaataattt tagtggcgtt ttatgtgata ccaataataa tgtcaatata ctttagtttt
    16741 acaaagtata atattattag tccagctact tttataggtt tagaaaatta taaaaaatta
    16801 tttacagatg aaacattaaa agtatctatt ataaatacta ttaaatttac tgttgttgta
    16861 gtaccttgcc aaactatatt atcattgatt ttggctgttt ggattacagg aaaaggaaat
    16921 agtaaaatag cttcatttgc taaaggtgct atatttatac cagtattatc atctatggtt
    16981 ttgataggta tggtatggag ggctttattg aatggagaag gttctattat atatcaggta
    17041 ttaggtgcct ttggaataga atcatctaaa cttttaggag atagtaagac agcattacct
    17101 acacttatgt ttatatctat gtggaaaaat atagggtatt ttatggtaat ttatatttca
    17161 gctataatga atttaccaaa acactgctat gaagtagcga aggttgatgg ggctacaaaa
    17221 cttcaagaat ttataaaaat aactgtacct ttattgaaac caactacaat aatggttgta
    17281 tttttagggt cgatatggtc attacaagta tttgacttaa tttatactgt gacaggtgga
    17341 ggtccaggta tatctactat gagtatagtt atgcatgcat ttaacttaaa ctttaaaaac
    17401 tttaattctg gatatgcaat gactgtagca aacgtattat ttttgttaat agcagtagtt
    17461 tcgattttac aaaataagct tgtaagacga gacaattcag atttttagaa aggtaggtat
    17521 aatatgaaaa cagcaagaag attattaagt gatggtttgc ttttatttat agcgtgtgca
    17581 agtttggtac cttttatata catgttgata atatcattga agataacata taactcatat
    17641 agtttggata tatctttttc aacagtaacc cttcaaaact atatagatat ttttactaaa
    17701 aaaggatttg cacaatattt ttttaataca gcaatagtat ctttttcagg tgtattatta
    17761 aatttagtat ttagtacatt agcaggatat tcttttgcga agatggattt taaaggtagt
    17821 gataagttat ttttatttat gataatgact ttaattatac cttcccaagt tacaatgata
    17881 ccactatata taataatgaa acacttagga tggataaata gttatcttgc acttattatg
    17941 ccaataccaa ctgcatttgg cgtatttata atgagacaag ctattttagg agtaccaaaa
    18001 gagttattag aatcagcaaa gatagatggt tgttcggatt ttagaatttt aatacaaata
    18061 gtgttgcctt taattaaacc agcattaatt acactagcaa tatttacttt tatgggagca
    18121 tggaacgagt ttatgtggcc tcttatagct acaactaaag atacaatgag aacattaaca
    18181 gttggattat ctactcttaa gacattccag ataactaatt atggacagat gatggcaggg
    18241 gctacaataa catttttacc tccatttatt ttctatttga ttttacagag taaatttgtt
    18301 gaaggtgtgt ctttgtctgg tataaaaggt taaatagtct aatttaataa tatataaaga
    18361 aacttatata aatatggagg aatttaaact atggaaattt catatgtact gaaagattgc
    18421 acattcaaac acaataaata ctcagttgta gaagctgaaa cttggaaaga taaggatttg
    18481 gatattgtag tagctaaagg tgaaaaattt gcatttcaaa ttatgttaaa agcaacagaa
    18541 gaatttaatt gtacaattga taaaagtagt gctatatcat ggaaaggcct gggaaataga
    18601 gtaaggttag cattaaatat gcctaatagt ttagaaaata attttagtat aaatatatta
    18661 ggatatgttc aagatgatac aaaggcattt gtgaatgatg ctattttaag ggataaagat
    18721 gtgttagtag agcaatattt gcctcaaaca ttttggattg aaggtcaagt accagaagat
    18781 tttgaagaaa ataaccttga tataggtatt gatatattta aaagctttgg atatgaagac
    18841 gaagaaaaag tttgtacaat tgatgttcct gtaaaagtaa gaaatgttgt gctaaagcct
    18901 ttagatgaaa gcaagttctt tttagattta tggcaacatc caagctgttt agctagaatg
    18961 tataaagttg agttatggtc tgatatacat tttgaaatag tcgataacta tttaaaagaa
    19021 ttagcatctt taggagaaaa agttgcaaca gttatagttt cagattatcc atgggcaggg
    19081 caaagttgtt ataaagttta taaaaatcca tcaaacttat atgaatataa catggtaagt
    19141 gtatcaaaag gattggatgg aaagattaaa tgtaactttg agagtatgga taggtatata
    19201 agcatagctg ataagtataa aatggctaag gagatagatt tatttggtct attaggtaat
    19261 tggtgtgctg gtgagtttgg aaatcctgta gaaggttata aggaccctat aagagtaaga
    19321 tattttgatg aaaaggataa agtatttaaa tttataaaca atacaaatga tttaaaagaa
    19381 tatataggat tagtattaaa tcatttaata gagtgtggat tatgggacag agtaaggatt
    19441 atagctgatg aaccaaataa tccagaagta gtcaaagagt gtatagaatt tataaattct
    19501 actgtaagca ctcatcaagt taaatataaa tcagcaactc acgaccaaaa ctttttagac
    19561 agagctaaag atgaaattga tgatatgtca ataaatttaa aacttactat acaaaattat
    19621 aaagatatag aaagtcttaa gaaaaaaatc aatgacaagg gtgggatatt gacttggttt
    19681 gtatgctgtt ttccagagaa accaaatagt tttttaagtt ctccttttgt agaaaataga
    19741 ataataggat ggtatactta ttactttggt cttgatggat ttttaagatg ggactataat
    19801 ttatggacag aagacccttg gaaagattca agttacaaat tccctatttg gaaagctgga
    19861 gatatgttct ttgtttatcc tggaaaagat ttaaagccag ttagaagtgt cagaatggag
    19921 aatttaagat ttggaataca ggactttgag ttatttacaa tgttagaaaa agaaaaggga
    19981 agagagtata tagaagttga gcttatgcaa gaattattaa ataaaaaaga aaatgctgaa
    20041 attaaaggtt ttggtgacat agaactagga tattcacttg ataattgggg atatgataaa
    20101 gttaaaaaat atgtattaga gttgttagac agagtataaa aaatataaga gtgtctcaaa
    20161 atgaactttt tagttcatga ggcactcttt ttgtatgaaa tcaatatagt ttcattgttt
    20221 caacaatgaa aaataggatt atttctattc taagacagcc ctttagtttt ttagttgtat
    20281 tcaatgtttt aaaactaact ataaacgcta taaagtttat aaatgacaat aattatatat
    20341 aacatgatgt actttaattt gctataatag ggattgaata atattatatg gaagataaca
    20401 ttgtttgggg ggattaatat aaaaagaata ggtattatta taatgttttt tttaagtata
    20461 tcttttattg gattttatct tgtaataaga gataacagtg atacagttaa gatgcaacga
    20521 gggataaata taggtaatgc actagaatca cctaaggatt ttccgtggga tgtaaaaatg
    20581 tcaaataagt tttttgatga tataaaagat gcaggttttg acacagtacg tataccagta
    20641 aggttttctg attatacgtc agattctgat aattttaaaa ttgatgaaga attttttaaa
    20701 aagattgata aatatgtaga ttatgcattg gataaagact taatagttgt gttggattta
    20761 caccattttg aagaaattat gaaagaacca agagttcata aagaaaagtt tttaaaaata
    20821 tggcaacaga tagctaatag gtatcaaaaa tatgataaaa aattggtatt tgaattatta
    20881 aatgaaccta aagaaaattt atattcacag ttactaaacg aatacattga agaagcaata
    20941 aagataatta gaaaaactaa ccctaaaaga actattatag taggaccata taatttctat
    21001 caaattgatt atttgaatga attaaatatt cctaaagatt ctaatatagt agtttccttt
    21061 cactattacg agccaaatga ttttgcattt caaggaaata tatatcataa gggatttgag
    21121 cacttaagca atattacttg ggaaggaaca aacgaacaaa tggattattt aaaaaaaagg
    21181 tttgatactg tggaaaattg ggcaaataaa aataaggtaa aaatcttctt gggtgaattt
    21241 ggaataacaa aagaggcacc agaagcttca agaagagcat ggataaaagc tgtaagagaa
    21301 gaagctgaaa aacgaaactt ttcatgggct tattgggagc ttgcatctgg atttggtata
    21361 tacaatcaaa ttgaagggac ttgggataga gatatattaa gtgcattaat agaaaagagg
    21421 tgattttatg aagaaaaatt caaaaaagat acttataact ttaactatta ttactaatgt
    21481 catatatata ttgtggagaa ttttttacac agtacctaaa gaagaaggga agtttgcctt
    21541 aatatgtgcc ataatacttc tttttgtaga gataatgggc atgatggaaa tgtttgtgca
    21601 ttattatggt atgtcaaata ttgagtaccc tgaaaaacca atcataagtg aagaacttta
    21661 tccacatgta gatgtattca ttgcaacata taatgaatct gttgatttgg tgagaaaaac
    21721 tgtaaatggg tgtattcata tgcagtatcc agataagaaa aaggtacata tatacatatg
    21781 tgatgatgga aatcgtgaag aaatgtgtat tctagcagaa aaaatgggtg taaattacat
    21841 aactagaact gaaagagaag gagcaaaagc tggaaattta aataatgcaa tgcaacatac
    21901 aaattcccct ttgattgcta catttgatgc agatatgata ccaatgcatg actttttaat
    21961 tgctacagta ccatactttt tgaaaaatga gcaagctaaa aaagatggag aaaaagagga
    22021 gtatgaaaag gttggttttg tgcaaactcc acagagtttt tataatccag acttgtttca
    22081 atttaatctg cactctgaag gaaggattcc aaatgagcaa gattattttt acagagacat
    22141 tcaattggct agaaatagga caaattctgt aatttatggt ggtagtaata cagttatttc
    22201 aagggaagca cttgaagaag ttgatggatt ttatacttat tctattacag aagattttgc
    22261 aacaggtatc ttaattcaaa gtaaggggta tagatgttat gctattccag aagtacatgc
    22321 atcaggttta tctcctacag atttaaaaag tcttattaag caacgtgaaa gatgggcacg
    22381 tggatgtata caaactggta gacgtctaaa tattttgttt aggagaggtt taggtttttg
    22441 gcagaaaata agttatattt cttcaattac ttattggtat gcaagtataa aacgctttgt
    22501 atatataatg gcacctattt tattttctgt gtttaatgtg attgtagtaa aatgtacgtt
    22561 acttcaagta ttggtatttt ggttgccaat gtatatttta agtagtttat ctcttaaaat
    22621 attttctcaa aatataagaa atactagatg gaccaatata tatgaaacta taatgtttca
    22681 gtctttaatg ccagcagtaa tacttgaaac gtttgcaata tctaaaaata aattttcagt
    22741 aacaaataag agtaaattag aagaaaatag aatgtataag tttttacagg gaatacctta
    22801 ttttatttac atggtcttat ctatcatagg aattttaaaa atgtttgtag caatatttaa
    22861 aatgagttca atgacatatt cagttgtatt attttggttg attggaaacc tttttaacct
    22921 tgttatggct acattattta tatcagggag acagcagtta agaaagtctg agcgttatat
    22981 tgctgaaatt gattttaagt taaagcaaaa ttcttatgtt ctttcttcaa aaacaattga
    23041 tatatcagaa aatgggtttg catttttgct agaaaatcca gaatatattt ccccagaaga
    23101 agagtttgag gtagagttta gagaaaaatc tggaaatgaa atgtatatag ctaatatgaa
    23161 agcaaagatt gttaatgttg tggaagtaaa ttcaaagtgg aaatatgcag cttatattac
    23221 acatattgaa gatagtgaaa tagataattg gatgtgtatt gttcacgata gaatacctac
    23281 tttaccaatg acaatatcta atcaactagg tttctttgat gatttgcaaa ttaatgtaaa
    23341 aaaacgtatt gaaaaaacta gaacattatc acgaagaagt ccaagaatca atatgaattt
    23401 tcagatggat ataaaaaata caggtaaatt aagaatagtg aattttaatt atcaatatgt
    23461 attattaaat tttgagaata aaagtattta tcctaaagaa atagcattgg aaattaatga
    23521 aaatattgtg ttagaatgtg atttgtgtga gggtaaaatt gatgaaagag gtatcttata
    23581 taaagtaaat aacattgaca gcataatgca aaatttcttt ttaagagatg aaatgatgga
    23641 ttggatatta caaaataaga aaattcttga ttcaaagcct agtgaaaaga aggaaaaaag
    23701 tatagatgag tttgaaccca tggaatatat ttaggagaga tgaaatgaag actttaaaag
    23761 tgtttttttg ttctacaaaa tatcttattt tggtgtttat tttttttata aatactactt
    23821 ttataagttc agcaacaagt gataaaattt tagatttatc ctttaaaaag atagagactg
    23881 acttaagttc taagattaca tatgaggata caggtgtaaa aatagaaacg gatagttcta
    23941 aaagcgataa ggaacgttat ttatatattt accaaaatat aaaagaaaat tggagcatgt
    24001 ataataattt ttatattgaa attcaaaata aaaataaaag ttctcagaga ataaatttaa
    24061 gcatacaaag taaaaatatg cttgaattta gattaaaaga aggtagtgaa gtatttttag
    24121 aaggtaaaaa tataatatat tcagataaaa taaaagaagg atgcatagaa gtaccaggtg
    24181 aatttgaagg caaaatttat gtgaacttta atagccttat aaatgaggaa agtaatgttg
    24241 tgctggactc aaatatgctt tcaaacattg ttagttgggg aattacgttt attccatctg
    24301 atgaagaaca taatattgtt attataaaaa agatatcttt attgtctgaa gagaaattaa
    24361 gatttttaaa taatattaaa ataattggcg atgaagaagt acaaatacca gtattgggtc
    24421 aaagtataag tcagtatgag gtattaggtc ttaaaagtga ttctaaaatt aagtactctc
    24481 ttatgggaaa acaagataat gtgagtatat ctcaaaacgg taaattaaca ttaagtaata
    24541 aagccaaacc tggacaaatt attttacaag ttaatgttga tgataagttt aaaattggaa
    24601 agaaaataac attaactgaa tcttggagta ttaataaaaa agataaggat ggtgttccat
    24661 atacattagt atctcctgag caatcaccaa cagtacaaga tatgaaaaaa ataaatttta
    24721 tgaataatat cataacgttt gttcgaattt tatttgtatc tctagtaata atatgctttg
    24781 gaatatattt atattggaaa aaatgttcaa aaactaagta aagagaggag gttagattgt
    24841 gttattttca agtttaattt ttttatttta ttttttacct ataactttgg ttttatatta
    24901 tgtatttagg tttaaccgaa cgatacagaa catgatattg cttgctgtta gtttattttt
    24961 ttatgcttgg ggagagccaa aatttgttgt tatcatgata gtttccataa taatgaacta
    25021 tatatttggt ttactggtag atagatatag ggaaagtaaa ataaaagtaa aagttttttt
    25081 agttttgatg tgtgcataca atataggtgt tttatttata tttaaatatc tagcttttgc
    25141 gctaagaaat attagtgaac ttattagtac tgaattgact atacctaata ttattttacc
    25201 tataggaatt tcatttttta catttcaagg aatgtcttat gttatagatg tatatcgtag
    25261 acatggagaa gtccaaaaga atccttttta tgtgggactg tatatagcat ttttcccaca
    25321 attaatagca ggtcctatag ttagatatga gagtgtggca gaacaaattc tcaatagaaa
    25381 agaaacttgg aataaatttt ctattggtac ttgcaggttc ataacaggtc taggtaaaaa
    25441 agtattaatt tcaaataaca tggctatagt agcagactat atttacacta tgaatagtca
    25501 aggagaaata gctgcatcac ttgcatggct tggttcaata gcctatactc tgcaaatatt
    25561 ctttgatttt tctgcttatt cagatatggc tataggctta ggtcttatgt ttggttttaa
    25621 atttgaagag aactttaatt atccatatat atcaaaatca attagtgagt tttggagaag
    25681 atggcacatt tctttgggaa tgtggtttaa gagctatata tactttccgc ttggaggttc
    25741 tagagtagca aataaagata ttatgattag aaatatgttt atagtgtggt tattcactgg
    25801 aatatggcat ggagcagaat ggacatttgt aatttggggg attttaaact ttgttttcct
    25861 aattatagag cgatttataa tgtttgaaaa gattgaaaat cacaatttta taaaacatat
    25921 ttatgcttta ttagtagtta attttggatg ggtattgttt aggtcaccta atttaaaaga
    25981 agcatataat tattttaaag ctatgtttgg aggcaatgga gctatatgga gtgattatac
    26041 atatatgttt ttaaaagaat actgggtttt ctttatattt gcatttattt ttagtgtacc
    26101 tattgcaaag aaaatcaata agtttgttgt tgaacaagct aaatatagta tgatatttaa
    26161 cgtattttat ccaatctcag ctattttatt gttttttatt tctgtgacat atttagtaac
    26221 tggaagctat aatccattta tttattttaa tttttagttg ggaggaaaag tatgaataga
    26281 ttttttaaag taagattaat aactgcaatt atgttcttaa ttattttatt cgctttctct
    26341 atctttaatg tttgttattc tggaaaagat acgatacagg aaataaaaaa ttcaatagct
    26401 gaaaaaagta gtttaaaaga tacaatatca tctattgata atacagtgaa tgacaaagta
    26461 atatacaaaa atacgttcat tgaaacttat ggctatttac aaaaattaat gggtaaaaat
    26521 gagttttcaa aatttagtgt tgtaaaagat acttctggaa agatgcatta tacatatttt
    26581 gctaaagctc caaatccagt tggtgtgata gcagataggg taagaaaatt ttctgatgaa
    26641 atggaaaagc aaaacactaa attggtttac ctaatgactc cagataagta tataaaagga
    26701 gtgacaaaat ttccaaaagg aataccttat tcttatagta atgagacagc agataatttt
    26761 ttaaatcaac taaaaaagta taatgtggac tatattgatt ttagagaaaa tattttaaag
    26821 agtggtattc caaaagatga tttatttttt aaaacagacc atcactggaa agtagaaact
    26881 tctttttggg catttggaga attagttgag caacttaata aaaaatacaa tatgaattta
    26941 gatgaaaatc attattatag agataaggac aattataatt caattgtata tcctaagtca
    27001 tttcttggtt ctatgggaag aaagactggt atcttatatg gtggtataga tgattttaca
    27061 ttaatttatc ctaaatttaa gactaattat acttattata cagattctaa atctcaaaaa
    27121 tttgaattaa aaggaaggtt tgaagagagt ttaatattat catatccttt taatgcagac
    27181 cttgatttaa tggatggtca atcagataag tattttacat atttattggg gaacagacca
    27241 ttagttaaaa taaaaaatat agaaaatcca gatggattaa aagtgctgtt tgttaaagat
    27301 tctctaattg taccaacagc agcatttttt tcctctgttt gttcatctat agatatgata
    27361 gaccctagat attatgatgg agatatacta gaatatgcaa aatcccatga ctatgacttt
    27421 gtttttatat ctgtatatcc ccaaaatctt acaaaagaat tttttccgtt ttgtgagtaa
    27481 atttctaaaa aagttttctg ataaaataaa tggctaactg gtaaaaagtt agccatttat
    27541 tttgagtgtt attctaagaa atattttttt tactaaatac ttctttatct atttttccaa
    27601 gtgattttcc tacagtaatt gatgatacca tagcaccatt aacatttatc atagtacgtc
    27661 ccatatctag tatagggtct attgcaagta taccaccaac taatggaaaa taagaaccaa
    27721 gaccaactcc agagataaca actgaaacgg ccattgtagc agttcctgga agaccagcta
    27781 ttccaaacga actaattgtt ataacaatta acaacataac ataaaagctc atatccatgt
    27841 ttactccagc catattggca agtgttacag ccattaaagc aggatatata cctgcacaac
    27901 cattcatacc catatttgca cctaaacttg ctgtaaagct tgatacaccc tcatcaactc
    27961 cgtgattttg tactagactt tctatcgtta caggcaatgt tcctaaacta gaacgtgaag
    28021 taaatgccag aagtaaagct tcagttgaat tttttatata agtaatggga tttagaccat
    28081 ttaaagttat aaaaattaaa tgtataataa ataatattgc aactgatata taaagagcta
    28141 atataaactg taaaacagat actattgaac taataccttg tgaagttatt gtatttgcaa
    28201 gcagagcaac cacagcataa ggcataaatt taataacagt catagacaca cttacaatta
    28261 ttttgtaaga tgcttctata aaatctataa atggttttat tgtctcaaaa tgttttttac
    28321 ttaatctctt tattgaggta gctataaatg tagcaaatat tattgtagct acaatatttc
    28381 catttgccat agccattaca ggattagatg gtagtaatcc tcttaaagta tccacaagag
    28441 agtttatttc tttaagttct tgattttcaa ctaaagtagg catattagct cctacaccta
    28501 acttcattat atttgcaacg ataattccaa caattgcagc tatagcagta gtagcaagaa
    28561 gcatacctaa agatttaaat gtcagagttc caagattgtt atctttcata tttataatta
    28621 cttttataat ggataagaaa actaaaggta ctactaacat ttttaataag tccataaatc
    28681 cagaaccaaa taggctatac cacttactta cttcttgtag ccaagtgact tgagttgtat
    28741 cattaggaaa accagcaaaa gcttgtatta tgattcccaa tatgagtcct agtactgtag
    28801 catatattgt tctcaatgaa aattttattt ttttcttttc aaattgtcta acaataaaga
    28861 acgtacctag aagtaatatt atgaaaaaaa ttgttttaaa atctgtaatc ataagaaatt
    28921 gtgataaaaa tgaactttcc aatatttttc tccttttatt taaaaataaa tttttctaag
    28981 tttaaatgat atcaatatat taaaaaaatg tcaatattat atcacaattt acttaaatag
    29041 agtaaaacaa gttgattttg aaaaatgtga ttaagtacag aaaaataggg tataataaat
    29101 gtttaaagaa tttgaattgt ggtaagaaaa taaaaaatag cttttataag atttgatttc
    29161 aaattaaaat atgttaaata aataactaaa aattattata aaaaatgttt tcatagaata
    29221 tatatcaaat gaaaagctta aaataagtga agaaattaac tagacatcaa aggagagata
    29281 aaatgagagt aataataatt ggtggtgttg cagctggtat gagtgctgca gcaaaattaa
    29341 aaagaataaa acctgagtat gaagttgttg tttatgaaaa aactgaaata gtatcttttg
    29401 gtgcttgtgg attaccatac tttgttggtg gattttttaa tgatgctgat gaattattag
    29461 caagaacacc agagaaactt agagaagctg gaattgattt aaatatattt agagaagtcg
    29521 ttgaggttga tagtgaatct aagaaaataa aagtaaaaaa cataaaaaca gatgaaatat
    29581 atgaagatta ctatgataaa ttgatgatag caactggagc aagaagtatt atgcctccta
    29641 taaaaaacat aaaattgaag aatgtttcta cattaaaaag tttatatgat ggagaatatc
    29701 taaaaaaact tttaagtaat gaagataata aaagagtgac aataatagga gctggtttta
    29761 tagggttaga agcagttgag gcatgtaaga aattaggtaa agatgtagat gtaattcagt
    29821 tagaagatag aatattgcca caagtatttg ataaggaaat gacggatgta ttagaagaag
    29881 aaataaatag acataatgtt aatttgcact tagatgaaat ggttgtagaa ctttgtggag
    29941 aagataaagt agaaaaagtt ataacaaaca aaggggaaat agatacagat gtagtaataa
    30001 ttgctactgg cgtgagacct aataccgagt ttttgtcaaa tactaatata aaaatgctaa
    30061 aaaatggagc tatagtagtt gatgagtatg gaagaacttc tgtagaagat atatactctg
    30121 ctggagattg tgcaactata aagaatatag taagtaatga aaatgtatat gttcctttag
    30181 ctactggagc taataaattg ggtaggatag taggagaaaa cctagcagga agagaagtat
    30241 cttatcaagg ttcactatct tcaagctgta taaaaattat ggatatggag gcagcatcaa
    30301 ctggaattac agaaaagcaa gctaaagatt taggcataaa tgtcaaatct aaatttattt
    30361 ctgattataa tcaaactcat tattatccag gaagaaataa aatatatgta aagcttattt
    30421 atgatgcaga tacaaaagtt attttaggcg gacaagtggc tgggtttaag gatgctgtcc
    30481 aaagagcaaa tgtattggca gctgctatct ttggaaaaat gaccacaaat caacttggaa
    30541 tgttagattt atgttatgct ccaccatttg caagaacatg ggatgtttta aatgtagctg
    30601 gaaatgtatc taaataatat ttagattata gctaatatag ttgacaagca attaagggaa
    30661 tgaatttacc taaactattt ttatcatggt aaaattcatt cctaaatttt tattccaaat
    30721 ttttattcca aatttttaga aataaggatt ccattaaatt attcatcaaa atttaaacgc
    30781 aagcttaatc tatcttctcc aaggatagta ttttcaaaaa cattagtagc attttctaaa
    30841 tacatactag gtatgtctaa agaaggacta aaatgagtca gtaaaagttg tttgacattt
    30901 ccaagttttg caagattagc agcttctcta aaagtcatat gtttgtttct aacagctttt
    30961 gatatatcta aatcatcacc atacatggct tcacatataa ataaatcact atcttttatg
    31021 aaatctggta ttgattctat aggtcttgta tcggttgtaa gacttatctt aactcccttt
    31081 ctcaattctc ctaagaccat atctggagtg tattgttttg aatctaaaac tatattttga
    31141 ccttcttgga gtttgttcca taatatttta ggaacttcgt ttttagttgc cttatctata
    31201 tcaaatttag ggtttcttct aaaattaaaa ctgtatccta aacactctat agagtgttct
    31261 aaactgattg ttgaaatttc taaatttctt aaaactttat tatctaaaga aaaattgcct
    31321 tgagggtttt ctataatttt taaagtgtat ggtacatatt ctactaaatt tctcatagaa
    31381 cgtatacaat cgactattcc aactggacca actatagtta aatcacttgt acgaccacta
    31441 tttccaattg ttgataaaag tcctaaaagt ccaaatatat ggtcaccatg taaatgggtt
    31501 atacaaatca agtctatatc tttaaagcca cattttttta gcttcataga tacctgagta
    31561 ccttcaccac agtcaattag tatttttcta cctttatagt ttataaatac agaggataga
    31621 aatctgtttg gcataggtac atttccacca catccaagta aagttaaatc tatcattttt
    31681 tcccctttct aaaattaaca caaatctgtt gtttgttttt attatgatat cctattataa
    31741 ttattttaac attaaatata acaataaatg tgtatttgag tcaagaaata ttgtcaaata
    31801 aagtttttat atagaaatgt ataaaaattt ggaaaacaaa aagtgaaaat tatatttagg
    31861 agcatttaaa ggaggaaaat ttaatgaaag tattaaagaa aattactatt ttgtcattaa
    31921 ttacattaac cttagttggt tgtagtaacg gagaagataa aaaagtggtt gaaggttcta
    31981 agaaaattca aaattctaaa aaagatgaac agcctataat agtacataaa gatgcaaata
    32041 tattgaaaga taggtatgag ccaccaaaag gatttaaaag agttgatgta gaaaaaggta
    32101 gttttgaatc atttcttagg aatactaagc taaaaaatta tggagagaaa gtaaagtatt
    32161 atgatggaag aactaaggaa tcaaaaggta tttatgatag tgtatttgat gttgatattg
    32221 gcaatagaga tttgcatcaa tgtgcagatg caattatgct tatgagagca gaatacttgt
    32281 atcagaataa actatatgat gaaataagtt ttgattttgt tgatggtttt aaggcgaagt
    32341 attcaaaatg ggcacaagga tatagaattt cagtaaaaga tagtggttca agctggtata
    32401 aagcaactga atattcaaca tcttatgaga gttttagaaa gtttatggat atagtgtttg
    32461 catattcagg aacgttatct cttgaaaaag aattggagcc tgtaaaagtt gaggatatga
    32521 aaataggaga tgtgtttata gttggtggaa gtccaggaca tgcaagtata attatggata
    32581 tggcagaaaa tgaaaagact ggtgaaaagg tatttatgat agcccaaagc tatatgcctg
    32641 cacaacaaac gcaacttctt attaatcgta aaaatccaaa tttgagtcct tggtatagtt
    32701 tagattttga aggtgatttg acaatacctc aatggacttt taaaagagac cagttgaaaa
    32761 gattttaaaa taataatttt atataataaa agaaaaatat agatgtgaaa aaatgtaatt
    32821 agattaaaac taattggata tggacaaaat taaattgata gataaatatt aaaaattaca
    32881 ctaattaatc ttactatagt ttaatattta tctaaaaagt tttatgtaaa atataaattt
    32941 tatattaaag ctaaaactaa tttgaggagg ctaatttata ttgaagatta gaaggtattt
    33001 aattttggta attataattc taattacaac aagtcagagt tttatttctt atgggctaga
    33061 gcgatttgaa gaaataacca tttttcacac taatgatata catggaagat atgctgaagg
    33121 tgatgaccat atacaaatag gaaatctagc cacattgaaa aaagaaactc caaactcaat
    33181 tttagtagat gcaggagatt gtttgcatgg acttccaata gtaaatatgg atagagggaa
    33241 aagtgcaatt gaattaatta aatgtgctgg atatgactat attactcctg gaaatcatga
    33301 ctttaattat ggaaaagata gactgtttga gttatctaaa agtgctaatt taggagagaa
    33361 tactttaaaa tttttatcat caaatatttt tgaaaatgga aaaaaagttt ttgatagtaa
    33421 tgatataaaa gaaatagatg gaataaaaat aggatttttt ggactctcaa cccaggaaac
    33481 aaagagcaaa actggattta aaaatgtgga aggattagag tttaaaaatc ctatagaaag
    33541 tgcaacagaa caagtaaatg aattaaaagg aaagggtgcc aatgtaatag ttgcaatatg
    33601 tcatattggt acaaatagtt taagtaagcc aaatagtata gatatagcaa atgaggttca
    33661 aggaattgat ttgattattg atggacacag ccacactaag tttgaaaatg gtaaacaagt
    33721 gggtgataca ttgattgtaa gtgcaggaca atatttagaa aatattggcg agataaaact
    33781 aactttggat actaatgatg tagaaaatat aaaaatagaa aataaatcag ttagattaat
    33841 tggaaaagaa gaagcattaa agtataagct tgactccgct gtaagtaaaa aggttgatga
    33901 aataaaagaa caacaagaaa aaatattaga taaagttata ggggtaacta caaatactct
    33961 agatggttca tatgaaaatg taaggacaaa agaaactaat ttgggtaatt taataagtga
    34021 cattttacta gataaaacta aggctgatat ttctttattt aatggaggga atattcgaga
    34081 tacgatagaa aaaggcaata taacaagaag agatattgta gatgtatttc cttttagcaa
    34141 tactatagtt accaaagaac ttacaggagc acagattaaa gatgtattgg agcatggagt
    34201 aaaactttat ccagaaaaaa gttcagcctt tttgcaagtg ggaggaattt catattactt
    34261 taatcctaaa caaaaagaag gcgaaaaaat aacaaatatt caaaaagaag gaaagccatt
    34321 agatttaaat aaaaagtatg tagttgcaac gaatgattat atagcatcag gtggagatga
    34381 atttccttgt ttttcaaaag agccagtact aaaggaattt ggaaatttag agagtgcagt
    34441 cattgaatat atagagtaca aaaaagaaat atcaaaaaat gtagatggaa gaattggtat
    34501 taaatctaaa gatgagttaa taagcaagaa tcatgagagt gatttgatat taagtgaaaa
    34561 tgaaaaaaat atcaatgata atgaagagaa tctatctaat aatataaaaa gctctgatag
    34621 tgataaaaat aaaagcttag ataaaacaag tgctagagaa agttctaatc taggaatatc
    34681 taaaagagaa gctaaagaaa aatctcctaa aacaggagat ttaggttttt caaatagtat
    34741 aattatattt atagtttcaa gtacattgat atgtttattg aattttaatc aaaaagagtt
    34801 aaaggataaa aagtcaaaat aaaaattaat gagcaaaaat tagtagatag taatttaatt
    34861 ttagataata gtaaacagca aaggaaatta gttatataaa tactaatttc ctttgtttta
    34921 tgttttaaca tgattacttt taaataaaat tgtattttat gaaaaataat tacactaaat
    34981 ggcatagtat tatggggatt tttagttttt attcaagtaa atgaaatcat attaacaact
    35041 tctgatatat ttacaaattg ataaaaaaat gattttatta gtatgatata atttagctag
    35101 tatatatgat gatggaggat atagggatga ataatatatt acttttggaa gatgacaaat
    35161 ctttaaatag aggaattagt tttaaattaa aaaaagaagg atacaatgtt tttagtgcat
    35221 ttggtataga agaagcaaaa aatattttcg caaaagaaga gatatgctta attatttccg
    35281 atataggtct acctgatggt agtggttttg acttttgtga ggagattaga aagaaaagtg
    35341 atgtttacat aataatgttg acagctcttg atgaagaggt ggatattgtc acaggatatg
    35401 atttaggtgc agatgattat atcacaaagc cttttagctt gatggtatta atatcaaaag
    35461 tgaatgcgct tatgaaaaga gtaaatacag taaaaaatta tactctatta gtttgtgatg
    35521 atttattctt ttattatata gaaaataaat taattataag aacagatgat aaggaagaag
    35581 agataatttt aagtaagaca gagacaaaac tattaaaata tttgatggaa aattctatgc
    35641 agacattgac taaagagcaa ctgttagaat ctttatggga tagtagtggt aattttgtag
    35701 atgataatac gattgcagta aatataagaa gacttagaca aaaggttgaa aaaaatccat
    35761 ctgaacctaa atatataaaa acagtaagag gggttggata tatgtgggga gaaaggagta
    35821 taaaaaaatg ctaaaaaata tgaacctcaa agatggttta agtgtgaaag atgattatat
    35881 aatgagaaga gttattgtat attctgtttt atctgtacta atagtaaccc ttgtatttat
    35941 actttttgaa tatagcagat tacataaatt gtatgaacat caaataaaaa acactcaaaa
    36001 tattgctgga atattgattg aaaaatatcc taatgatgaa atagatatag taaattctat
    36061 ttataattct gattatagta aggtgtctac tggagaaaag gcatttaaga aatttggcta
    36121 tggattggaa aataaaatgt caaatgataa aaattttagc ttatacttaa aatctttttt
    36181 taaggaatca ttttttattt ttttaatgat gtttatagta gttataggta taatatatta
    36241 ttttataaga tacataaata aaagattgag taaaatttat tttatagtag ataagatgtc
    36301 acaagaaaac tacttgaagg acactactga gtcagaagaa actggttatc tagtagaata
    36361 taatgaatac tttaaagaag gcatatttag taagataaat aactgtctat atgagcttaa
    36421 tagaagttta aagataaagt ttattaaatt agaaaaagaa aaagaaagtg taaaatcatt
    36481 agtaacagat atttcccatc aattaaaaac tcctttagca tccttaaaat tatacaatac
    36541 attacttata gaagaagaat tagaagaaga tgaagccaac gagttcttat taaccaataa
    36601 ggcttctata aataaattag aaaatttaat aaattcttta gtgaacatat caaggttaga
    36661 aattagcatg ataaacataa aaaaagaaga taatgatata aaaaatacta ttctaaatgc
    36721 agtaaaaagt gttaccccta aggctaagag caaaagtata acattaaatt taaatgaatt
    36781 taacagtaga attataccac atgataaaaa gtggactgag gagagtattt ttaatgtatt
    36841 agacaatgct gtaaagtata cacaggtaaa tggagaaata aatatatctg tagaagaaac
    36901 tgctaatttc tttaaaatta taatagagga taatggcata gggattgaaa aaaatgagtt
    36961 taataatata tttaaacgtt tttatagagg ttctgctgat gaaattgaat ctgtagaagg
    37021 tagtggggta ggattgtatt taagtagaaa gattttagaa gaacaaggtg gaaatattat
    37081 agtttcttca aaagtaggaa tagggagcaa gttttctttg tttcttacaa ctatgtaata
    37141 ttaaatgaaa ggaaaatgta agcctctaca tttattatat aagtataaac agctccagaa
    37201 ataaaaaatt aataaaatat ggagtaatat aacttagaat gagaggataa ttttatgaaa
    37261 atactatata ctgaaaattt atcaaagcat tatggaaaag gagaaagctt agtaagagct
    37321 ttggacaatg ttgacttaga aataaatgaa ggtgaatttg ttgcaataat aggaaaatca
    37381 ggttctggaa aaagtacact tttgcatatg attggaggtc ttgatatacc tacatctggg
    37441 aaggtgtata tagataataa aaatatattt acattaaaag aagaagaact tgctgtattt
    37501 agaagaagaa aaatagggtt tatatttcaa agctataatt taattccatc tcttaatgta
    37561 tgggaaaatg tagtgttgcc aatcggttta gatggtagag aagtagatga atcttttata
    37621 aaagaacttt taaaatcatt aggacttgaa aataaacacg acgttttacc aaatacatta
    37681 tcaggtggtc aacaacaaag ggtggcaata gcaagagcat tagctacaag accagctata
    37741 atacttgctg atgaaccaac tggaaaccta gattctaaaa cgtctgatga ggttatgagt
    37801 atattaaaat ctatgtctaa gaagtacagt caaactctag ttatgataac tcatgatgac
    37861 agtatagccc aaatggctga tagagttata ttcatagaag atggcagggt gtcaaaggtt
    37921 ggtgataaaa atgattaata ctactaaaat agcaaaaagt aatttaaagc aaaataaatc
    37981 aaaaagtatt ctaataataa ttactataat actttcaacc ttaatgcttt cttctattgg
    38041 aatttatatt gttgatgcag gagcatatca aaaagaaaat actataaagt attcaggcaa
    38101 ttatcaaggt atattagcaa atgtagatga aaaacaggct gatatattga gtaatcatgc
    38161 agatgttgaa ttaactggtg aaatgaatgg agtaggtgtt gaaaaactgg aagatgattc
    38221 aaatatttct ctagcataca tgaatgaaga tgctttgaaa ttaaatagtt ttgaatttat
    38281 caaaggaaaa ttgccatcaa aagagaatga aattgtgtta gatagtggag ctttaaaggc
    38341 acttggttat aaaaataaag atttaggtga aaagataaaa atatcttatg atgactataa
    38401 aaatgataaa aaaattgaaa aagagttcat tataagtgga atcttaaaaa ctagcgaaat
    38461 atctgaagca ggtaaatatt attatgctat tatatcagaa tcatatatga gaaatactag
    38521 agatatgtca caagaagatt ttaatattta tgtgaaattt aatgataaaa gtaatttatc
    38581 aatagagcaa gcaaaagaaa aattagataa aattgctaat gatataggac tggatactat
    38641 taatactgct gtaaatgaaa attatataaa tgctttaaaa ccagatatgg aaacgattat
    38701 gggtggagtt tttgtagggt tagtaattgt attatctagt attttagtaa tat
//
LOCUS       DAEPIN010000031        38709 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.031, whole genome shotgun sequence.
ACCESSION   DAEPIN010000031 DAEPIN010000000
VERSION     DAEPIN010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 38709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38709
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.031"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            complement(191..802)
                     /locus_tag="KR250_001832"
     CDS             complement(191..802)
                     /locus_tag="KR250_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBH0067768.1"
                     /translation="MKMVKKLILSTIMALVLVLTIGCSNAEEKVKSNFEANLAALEMY
                     KYDEKGISDALTEAEYKQIQTYNSKDKVSMIAQLTPLYGKENAEKIYETIVKLIQGVE
                     KTVTVESSSKDKITLKVVSKALDTSKVMSEANTLVKSYVTDEKNYDKKEKEVQDDMTK
                     MIIDLYNKKPTKEVTTMITYTKKDGKWQPPSNIQKLCEGIYTI"
     gene            complement(894..1883)
                     /locus_tag="KR250_001833"
     CDS             complement(894..1883)
                     /locus_tag="KR250_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HBH0067769.1"
                     /translation="MLLLKKDDIKKVFTMRDAIEADKEAFRIYCEGKSVNPLRTNISV
                     PSQDASMLFMPGYIEELGCGGIKIVSVFPKNAQKGKPVIPATVLLLDGETGEVSAVLD
                     GTYVTQIRTGAASGAAIDVLAKKDSKIGALIGLGGQGEPQLEAMIEARNLDEVRVFSR
                     NKESREKFAEEMNLKLSKYNTKIVAVESSDEAINNADVIVLATPSKQPVLNGNLVKKG
                     ALISAVGSYMPDMQELCPNCLTRASKIFFESTDAVLSESGDILIPLKEGKISKDDFSG
                     DLGNVINGTIPGREDDDEIIVFKTVGIGVQDVVTAKRIYDKAKENGIGIDWKW"
     gene            complement(1950..2813)
                     /locus_tag="KR250_001834"
     CDS             complement(1950..2813)
                     /locus_tag="KR250_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HBH0067770.1"
                     /translation="MKFFKVDTKAKVLRYVFVLTLTIMAVLAIKHDYKYNNLRKDASI
                     NLNSNKEAVTVMSNQSLPKGNTNSKQTLSVSTIGSKTIVKNDEYLESTINMPVITNSN
                     KIVERSTNDKIKNDIFEFYNKSYKEAKQYLKDNPDEKNKFVANVDFELKKNTDSALSI
                     KVRYYTYSGGAHGFYQDIAYNVDMRTGKFLELMDLFKDNTKYKEVIDEEIKRQIAELE
                     KKDEENIGIYNFKGIKENQNFYLQDENLVVYFDLYDITPYAAGIPEFSINKALISSML
                     KPEYVDLFDLK"
     gene            complement(2919..3515)
                     /locus_tag="KR250_001835"
     CDS             complement(2919..3515)
                     /locus_tag="KR250_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HBH0067771.1"
                     /translation="MERFNNMVIAIGASVGGTEAILEIIKDLPKSTPGIVVVQHMPAI
                     FTYMYAQRLDKQCIMNVREAKNNDRVEQGNVLIAPGGYQMKLCTDKQGYYVTCEKGER
                     ISGHCPSVDVLFDSVAEVAGKNSIGIILTGMGYDGANGLLKMKENGSFTIGQDENSCI
                     VYGMPMVAFDRGSVMLQLPLGEISNCLIRHINILKQKD"
     gene            complement(3555..4358)
                     /locus_tag="KR250_001836"
     CDS             complement(3555..4358)
                     /locus_tag="KR250_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HBH0067772.1"
                     /translation="MIKLTDEEFIVLVNYVKKEYGIDLSKKRALIEGRLYNTMIEKKL
                     SSFSQYMNLLFKDKTGNEAINLINRLSTNHTFFMREPQHFEFIQNSILPFWEENNKIR
                     NINIWSAGCSSGEEAYSIAMTLDDYFGYNKELWNIKIIATDISTNSLEKAKRGIYIES
                     NVNNVPELWKKKYFIDNKDGTFKICDKIRKSVIFKPFNLMNDFSYQHFDLIFCRNVMI
                     YFDLKTREELINKFYNATKKDSFLFVGHAEVINRSSTKYKYVKPAIYKR"
     gene            complement(4394..4834)
                     /locus_tag="KR250_001837"
     CDS             complement(4394..4834)
                     /locus_tag="KR250_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HBH0067773.1"
                     /translation="MAKDEIIKQVLTFYVNDVIYGIELENVIETIRFQSITYVPCLPT
                     YISGVINLRGRIIPVINMHIKYNLPKADYNERTCIIITKVDEYQVGIIVDKVVDVIHI
                     ENLNLLETTNSNNTNINKDIKEIAKVEDNSILILDIRKFLINSI"
     gene            complement(4847..6949)
                     /locus_tag="KR250_001838"
     CDS             complement(4847..6949)
                     /locus_tag="KR250_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HBH0067774.1"
                     /translation="MRQMLDMFIFETSLLLEQLDEIMIQIEKEKVFTHNNINETFRIM
                     HTIKGSASMMGLDNISALAHSVEDLFYAIREGKIEVLDNDLLFDLVFQSLDFIRRDID
                     ELQSRGCISSDYSNFIEGIEEYVSTLELSSSMKSKDNANLESKDEKEVIDSNFEYKED
                     SDVVKIIFEQGCMMENVRAFMLVFELRDYCEFIDFYPKDVETNAESTEYIKSYGFYIQ
                     FVAKEDKAIVYKTIEDNLYVKEYINISKKEIEMKNTSNNDAVENKLKDNNKVSKSYQN
                     NYTEDEIQEDVLNEKTDMEKDSLSEENEHLPISKQSIMSVNLEKLDLLQNIVGEIVIM
                     ESMVVNSVNSTGVDIDNFNKATRQLHKLTDELQDIVMSMRMIQIAGVFQKMHRIVRDM
                     NKKLDKNVELITIGAETEVDKSVIDNLAEPFMHLIRNAMDHAIEPIEERIAKGKEEVG
                     KIVLSAENLGGEAVISISDDGRGIMRDKILAKAKEQGLLKHQSNEYTDDEVNSLIMTP
                     GFSTKESITEFSGRGVGMDIVRKSIEKVGGSIEVKSKQDEGTTFTIKIPLTLSIVEGM
                     KFKVGDSLFTLPISSIRRTFKLTDLNQIITEGNKTELITIYGACIPIIRLHKIYNIQT
                     KVTNLMNGNMIIIENEHKAVCIFVDEIIGNQQVVVKPFPVYFNRFNIKNKGLSGCTIL
                     GDGSISLIIDADSIIEIY"
     gene            complement(7210..8913)
                     /locus_tag="KR250_001839"
     CDS             complement(7210..8913)
                     /locus_tag="KR250_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HBH0067775.1"
                     /translation="MKREVKLSKLAIVLLIIIVLAVLAGIFSSISSYKAFEKVEKSIN
                     DMNNLRTQSQYIKDTTREISDVLRRYVFTGEKKNKDIYEKELYGKTRESALNKLEKIG
                     LTKSESNKILEAKEISDSLVPTEQSAIKAVESNDKSKAQSIIFGSDYINSQEKMDSLI
                     NEFQEDIVTRSNSKIVEVKKELQSLILRTIFILSLLIILTIIEYTVIKLKIVIPINKV
                     EKHFSRIASGDLRTSIDVEESKSEIGLLVSSVKKMQSMLLNYIDLIDSTLTSIAKGDL
                     RVEIDQEFIGDFKSIKISLESIINSFNGDFSEINLSAEQVASASEQVAAGAQALSQGA
                     TEQASSVEELASTINEISDGIKKCADSASLACEVSDQSASQVGFGSRCMDDMMTSMGE
                     ISEKSSEISKIIKTIDEIAFQTNILALNAAVEAARAGQYGRGFAVVADEVRSLAGKSA
                     KAAQNTAILIEEAIKTVENGVRIAKETANALDLVVNGVEKTTNYINGISSAVHSQEVA
                     ITQILSGIEQISIVVQMNSATAEESAAASEEMSAQAQILKELVEKFHLKESEVRESSW
                     V"
     gene            complement(8939..9898)
                     /locus_tag="KR250_001840"
     CDS             complement(8939..9898)
                     /locus_tag="KR250_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBH0067776.1"
                     /translation="MKNKQQGTILVVDDSVLMCDLIDKTLTKDNFKVKKAFNAIEAKE
                     FIEEFIPDIILLDIILPDNSGFEFCKEVKSNARTADIPIIFITSKNTDTDIVKGFGVG
                     AIDYMVKPFSMTELKARVIAHLEVKKSQDKLKKINNELESSLEKLNRLVVRDYLTGLY
                     NRRHIINQLMEQRRINKYSETSISLILGDIDDFKVINDTYGHEAGDFVLTVISSIIKK
                     NCRQIDTVSRWGGEEFLIMLTNTPIEGAKILSERIRKEIKEFDFNYKEHKIKCTITLG
                     VVEVNSNISLEENISLADKAMYEGKNLGKDCSVVSTMKGLKKI"
     gene            complement(10034..10552)
                     /locus_tag="KR250_001841"
     CDS             complement(10034..10552)
                     /locus_tag="KR250_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-binding chemotaxis protein CheW"
                     /protein_id="HBH0067777.1"
                     /translation="MRFPDDPYLDYGLIDEGIKYLKFRVDEQDFGVELEKVIEITGNQ
                     EIIFIPELPTYSKGIINLRGKIIPIIDMRLRLKKDELMTTNCMYIVVTEIKDLVVGFV
                     VDRVDDIVSLEKSKISPPPRVTIEYSDYFVSGVGELEGKIITLLDLEKLLTDKDEVLI
                     DELINGFDCTIM"
     gene            complement(10595..11080)
                     /locus_tag="KR250_001842"
     CDS             complement(10595..11080)
                     /locus_tag="KR250_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HBH0067778.1"
                     /translation="MNREIVVNIADMKIAYRPNVLVTYALGSCVGVCLIDKVAGIGGM
                     LHVMLPYSKDAINIENKCKFADTGINELIKSMENIGANRIYIKAKIAGGAQMFLGSSN
                     SVIASIGHRNVEAVKKTLLGLNIPILAEDTGMNYGRTIRFYTETGELLVDSINKGKKK
                     L"
     gene            complement(11077..11694)
                     /locus_tag="KR250_001843"
     CDS             complement(11077..11694)
                     /locus_tag="KR250_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheC"
                     /protein_id="HBH0067779.1"
                     /translation="MINYLELDDVHIDALREIGNIGSGNAITALASMINSNVEVLIPM
                     VKILEYNEATNLLGGPENKVVCILLDMKGDINGMFMFLLDESITQLMLSSLFNKEEAF
                     LDEIEAIEISAIKEIGNIMASSYVNAIASMLNMTISVSIPDICIDMVGAVLNVPMIRF
                     SDVGDKVLFIENKFKMSDNYFTSHILMIPEMSSLREILVRLGLEV"
     gene            complement(11759..12121)
                     /locus_tag="KR250_001844"
     CDS             complement(11759..12121)
                     /locus_tag="KR250_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBH0067780.1"
                     /translation="MENKILIVDDAIFMRMIIKEALSKSGYNNLIEANDGEEACNIFK
                     YEKPDLMLLDITMPKKDGLEVLREIKKLDSSAKVVMCSAIGQENMIVEAIKLGALDFI
                     VKPFKTETLVKVVDNALK"
     gene            complement(12865..13362)
                     /locus_tag="KR250_001845"
     CDS             complement(12865..13362)
                     /locus_tag="KR250_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HBH0067781.1"
                     /translation="MNYEIVEINEKIVIGISKETTNKDGQAVNDIGELWKKFMGKGIY
                     NAIKGKKNDKTIGLYTDYQGDFTSPYSFVACCEVNSNSDKEKNLEELNTNNTVGESII
                     SKVIPAGKYAKFVIVGGQKEVGDFWFEFWQMDFDRTYISDFEEYQCNTFDTKKQEVHI
                     YIGIK"
     gene            complement(13537..14448)
                     /locus_tag="KR250_001846"
     CDS             complement(13537..14448)
                     /locus_tag="KR250_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family transcriptional regulator"
                     /protein_id="HBH0067782.1"
                     /translation="MKINRLTEIIVILLNKKLVTAKELADRFEVSTRTIYRDIETLSM
                     SGVPVYMTKGKGGGISLIEEYSIDKAILSKKDKESLIVALKTLQATKYPEINSVVNKI
                     GSIFGEQNFSNWIEIDFTEWGSNFNEDDKFTKIKEAILRRNTINFNYVNSLSSQTNRT
                     VEPLKLMYKSKTWYLYGFCKLKDDFRIFRISRIRNLSIKDEVFSRKIIEEVCLNDSKV
                     IKENTITLKLRFKEKMLFRVFDDFNKDLITKNEDDTYDVITEFPIGEWIYGYILSFGD
                     NVEVLEPKDVRDNVITRLRELSKIYSL"
     gene            15373..16224
                     /locus_tag="KR250_001847"
     CDS             15373..16224
                     /locus_tag="KR250_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3100 domain-containing protein"
                     /protein_id="HBH0067783.1"
                     /translation="MEINKKHFIQVFLTCLILVVICEFIGQRTIAIGKASIVLFPMLY
                     AVIIGLIITPDLLGKKIKALKKVVGEKEINIAGEVVGIALVMLGIKYGTTVGPNIDKI
                     IQAGPAFIAQEFGHILAPIVALPLALMFGMKREAVGATASISREPSLGVISEKYGINS
                     PEGSGVLGTYLMGTVLGTIYFSVLGSISIYSGLHPYALGMACGVGSGSMMTAAAGSLS
                     TMVPPEMADTILAYAATSNMMSSITGIIFLVFVSLPFTNFMYKILEPKFSRNKKTKEI
                     KSTEGEC"
     gene            16228..16698
                     /locus_tag="KR250_001848"
     CDS             16228..16698
                     /locus_tag="KR250_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0067784.1"
                     /translation="MFANLNLKKSSYQLLVMSVIGVIILIGQRISVGTSIVTALPGMV
                     MLILAAMAAMIIKDLFPKSIFPAFGFATIIGLLLSMPYSPTSEVFLTNTNNINFMAIT
                     TPLLAFAGISVGNKIEALKEMSWKIVIISLIVFTTIFFACASIAHIVLSIQGKI"
     gene            16711..18021
                     /locus_tag="KR250_001849"
     CDS             16711..18021
                     /locus_tag="KR250_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBH0067785.1"
                     /translation="MNKEQLKSLVIKTIDENREKILKIGQGIYKNPEYGYKEFKTTEA
                     VSNFFEQELNLSVEKNIAVTGCKADANSSKKGPHISILGELDGISCKEHKDANEIGAS
                     HTCGHNIQIAGMLGAAVGLVKSGVLEHLDGKVSFMATPAEEFIELEYREQLKKEGKIT
                     YFGGKQELIKRGYFDDIDISMMFHASDLGEDKALVGPESNGFVGKKVKFIGKESHAGS
                     APHDGINALNAAMLAINNVNALRETFKESERVRFHPIITKGGDIVNVVPADVHMESYV
                     RARTINGMIDANERINKALMAGGMAVGADVEITEIPGYLPILRYKSLDNLFKNNLEDL
                     GLKGKVIDGGDFTGSFDFGDVSHIMPTLHPMIGGVKGALHTRDFEIVDEDLAYIIPAK
                     AMALTVVDLLFDNAKDAKDILDTFTPVMTKEEYLTFLEKHDKTL"
     gene            18233..18979
                     /locus_tag="KR250_001850"
     CDS             18233..18979
                     /locus_tag="KR250_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBH0067786.1"
                     /translation="MFNAGNRIINTWLYPIQDGFVLIDTGYENGFKHFKKRISKFNIK
                     IKDIRYIFLTHAHDDHVGFLNQILDESPNTKIVMHDKALEILYKGQNSFIGGCTSRTA
                     FLFCQIMKLFGKGEHSFSPLKQEFEKRCILVSDKNRKEIEKELNGKIIDTPGHTADSI
                     SLLIDEGVLFCGDSAMNGFPSTHKITIFAESKLEFIQSWKTIINTKPKMIYPGHGKPF
                     EYSKLEQNLIYVNKINLYPLHNSKDIHRFL"
     gene            19341..20882
                     /locus_tag="KR250_001851"
     CDS             19341..20882
                     /locus_tag="KR250_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HBH0067787.1"
                     /translation="MNDNVVKKKNFFDRTLNRIETVGNKLPDPVTIFLGLCVLLLILS
                     SLVGSMGISVVHPGTGETITAVNLLTVEQLQILLGNIVSNFQGFAPLGLVLVTMIGAG
                     VCDKTGLMTATIKASVSKIPETRVTLVVMTIGMLANIASDAGTILFPPLAALVYLGVG
                     RHPLIGLFSGYAAVCLGFAANIMISVNDILAASFTVPAAQMIDSGFQANATMNLFFMI
                     ASTFVLILLATWVTEKIVAPRFGKYEGNAELDVDGSLSDVEKKGLKKAGISILIYVAI
                     IVALSVIGKKPFLADPETGDLLSNNAPLMKGMVPIIALAFFIPGVVYGKTIGKIKKDK
                     DVVSIMASAMSDMGGYIILAFAASQFLQLFTNSNLGLILAVKGGEFLKSAGINGPLLL
                     IGFIILSCFINLFIGSASAKWAILAPIFVPMFMMVGYNPALTQMAYRIGDCITNPLSP
                     LFPYFPIILAFTRKYDKDAGMGTVIANMIPYSISFLIVWTILLILFMVFNIPLGPGIL
                     PSYSM"
     gene            20917..22023
                     /locus_tag="KR250_001852"
     CDS             20917..22023
                     /locus_tag="KR250_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HBH0067788.1"
                     /translation="MLDKNRLINNFMDMVRIDSPSNQELEMSKWLVNYLKERNIDAII
                     DDAGEKYGGNTGNVIAYIKGEEGSRPLCLCAHMDQVQPCLGVKPILDGNVVRSDGTTT
                     LGADDKAGIAAILEALEHVITEKIPHRDIYLCFTICEEAGMHGVKNFNPDNLPCKDMV
                     ILDSGGAIGSIAYKAPAQQSIKISFHGKKAHAGIEPEKGLNAILVASHAISNMHIGRI
                     DSLTTSNIGKIEGGGATNIVTDKVTLTAEIRSHIPETLEYELNHMEKCCKDAASKFNT
                     TYTFEHSMSYPSFELSRDSHVFKLSEEAIRQVGVVPNPMVIGGGSDANILANLGYNCA
                     ILSLGMYDVHTVNEYVNIDELYDTAKIVYHMIKL"
     gene            complement(22354..23640)
                     /locus_tag="KR250_001853"
     CDS             complement(22354..23640)
                     /locus_tag="KR250_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBH0067789.1"
                     /translation="MIVGIIGPSDSGFKIKDDLKQIDSDLKTKIYVREKVVDTIEVIS
                     KCEDECDAIIFTGCAVYEFIKSKYEISKPHSFVPRSGTSIMKAFWAIKSANIKLDKFS
                     IDVVDRYVVEDALKEFDIKATSVFCNPFSLEVGESELVEWHIKLFEENKTDIMLTGFG
                     AVYNELKERGYPVFRLQATIQLIKESYDKVKSEYALSKARFSQIAVEILSLIDYKEKI
                     DNYYSDMIKKSDMDKLVVNYVRSIQGSLFLLGRNDYVVFVHKGVADNEYNYDKLFNLK
                     REIKDMGFSLSIGIGTGVTAYQAENNAHKALRHSIDSKEIGIYLVDEDENIRGPLASE
                     NELNYSLMLSDEKVIEISKKTGMSCESVAKIIAINENRKSKIYDSKELAEYLNVSERS
                     ARRILNKIVNAGLGRICAKETSIGGGRPKNITEILF"
     gene            complement(23763..26861)
                     /locus_tag="KR250_001854"
     CDS             complement(23763..26861)
                     /locus_tag="KR250_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HBH0067790.1"
                     /translation="MDVINLIDELDEIVYVSSLDTYELLYINEIGKKAMGLNDISHLK
                     CYKALQGKDSPCEFCTNSILKEDEYYIWENTNTRTNRHFILKDKLIKWEGKHARLEIA
                     LDITEKEDISKSIAEKLEIKELLVGCIKHLITESNFSDAVNLVLTNIGKFHKADRAYV
                     FEVSEDRTKVTNTYEWCNEGVDEQKQALTNLDINELKNWFLLFEQKGFVIIDDIENIK
                     EEQPIEYEILKPQKIESLMTSSLKQDDKVVGFIGVDNPHKAVGDTSLLTSLSYFLLTE
                     MKKRKVENKLNYMSYYDFLTGAYNRNKYIKYIDNFQKGKVKSIGVVFVDINGLKDIND
                     NFGHANGDSAIVSACVIIKKYFHSYNVYRIGGDEFVVLCEDISKQLFEELVSWLSEEF
                     KDEMIYSVAVGSIWSDDNIDIHKLIKVADERMYQDKKRYYNKNKLRNNEMKVISDLAD
                     STNNILFKHKELVDKVYFDDLMIKGYSKAYEKLAYNTYDIILSICIDTNLITPIYQSE
                     SNIFSFPIKENLEKTFSYIKKYLIHPDDIILFNDNHLLKSLDSLNNSKQDDLELNFEY
                     RRLGIDKKYYWALIYMLKISSKNKLGEKQTHILVAIKNIDYLMQLHKNQKDTLTGLYN
                     YESFCEQISLMLKRYPDKRYAIIMMDIDKFKVINDIYGVRGGDKALIYVAELIQKNIG
                     SNGICCRMYADVFCIFYESYSDRDIHKVIYKIKNSLNRKKFEYMLVPCFGVYRILDNH
                     IPITNACEMAGYAHKKVKKQSINNYIFYDDTIRNDALEEKQMEREMEYALENGQFHVY
                     LQPKYNLNANKIVGAEALVRWVHPEKGIIPPIKFISLFEKNGFIIRLDMYVWEQVCSL
                     IRKWIDLGEEPVPISVNVSRLHLHNPHFKEIVLSLINKYSIPKSFLELELTESLFIKN
                     IKLFTKIIADLRMDGIVLNMDDFGSAYSSLNMLKNINIDTLKIDCGFFNEEGITDREK
                     IVLKHTIAMAKDLDMDVVAEGVETKEQAEFLVSLDCVVIQGYYYCKPIPSSDFEKLLY
                     QK"
     gene            27586..27738
                     /locus_tag="KR250_001855"
     CDS             27586..27738
                     /locus_tag="KR250_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0067791.1"
                     /translation="MKIFSFLLFVVGICFCIAGFMGHSSAFSGATLSFFATTMIAVRQ
                     VKTKKK"
     gene            complement(28031..28735)
                     /locus_tag="KR250_001856"
     CDS             complement(28031..28735)
                     /locus_tag="KR250_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0067792.1"
                     /translation="MEIKPLVLIVEDDKPICKFIKVSLETQNYRCVETDNGGTAISLI
                     HSLDPDLIILDLGLPDIDGIEVIGRVRACAKTNKIIVVSAREHERDKVEALDGGADDY
                     LTKPFSVTELLARVRVALRNKAQQDNINNDAPKSFEVKNLKIDYENHIVSINGEEIHL
                     TPIEYKIIELMSKYSGRVLTHKFIIDKVWGNYYESENQSLRVFMASIRRKIEKNPAQP
                     EYILTEVGVGYRMADE"
     gene            complement(28726..30333)
                     /locus_tag="KR250_001857"
     CDS             complement(28726..30333)
                     /locus_tag="KR250_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HBH0067793.1"
                     /translation="MKKERLQHEDLKEYDYDEDGYLSKKIIIKDFIKTILVMTIATGI
                     SVVFEKVGFNESNIILVYILGVLFATSITYGYFFGILSSVIGVLSFNFFFTHPLYTFL
                     AYRKDYPMTFCIMLMAAIITSTLTSRIKREVRLSHKREKMIRLLYENNKTLLKAKNKN
                     QIIEFCGSNLVNILNRTVIVTIADENNNLGKPSIYICNNDGSENIFHSVLEKEAIKEA
                     FRTGNPVGIGTKYCKNSSAYYFPIKGQESNVLGVIGVACFKIDIITENEKILLEAVST
                     QVALAIDRENLFEKNKKANLEAERERLRGNLLRSISHDLRTPLTSILGSSSTILENDD
                     VIDKETRVELLKNIYEDTSWLTHSVENILSMTRIDEGKLDIEKRPEVVDEIIAESILR
                     VKKFANSRDIKTNIPDEIIIVHVDVLLIEQVLVNLIDNAIRHTPKNSKIELTVKKESN
                     QVLFEVADNGKGIPNEDIGNIFNRFYTKNKSRNLERRGIGLGLEICKSIVEAHGGEIV
                     AFNNPSGGATFRFSIPMYDEEGKDKWK"
     gene            complement(30587..31765)
                     /locus_tag="KR250_001858"
     CDS             complement(30587..31765)
                     /locus_tag="KR250_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0067794.1"
                     /translation="MEKLSDKRRVKAVSMETFVFIVLLAVGFGYVGSIMGAGMMFKVI
                     MSTAHALLLDTVFLIMAMAVLAGALSALLSEFGVISLVNKIFKGLMRPIWGLPGASIA
                     GVVATYLSDNPAIIPFAKDKTFTQYFKKYQVPALCNLGTAFGMGLIVTTFMIAQGKEY
                     VLPAIIGNVGAIIGSIISVRIMLTFTKKYYNYDPKNDTEKQINDKGAKLEEFREIRDG
                     NVFQRTLDAILEGGKLGVEMGMAIIPGVLVVCTLVMLLTFGPSTDPATGQAVYTGAAY
                     EGIKLLPAIGDKISFIIEPLFGFTSPEAIAFPVTALGAVGAAISLVPEFIKSGAITPN
                     DIAVFTAMGMCWSGYLSTHIGMMDALDARPLAGKAILSHTIGGLCAGICAHFIFMLVG
                     "
     gene            complement(31991..32485)
                     /locus_tag="KR250_001859"
     CDS             complement(31991..32485)
                     /locus_tag="KR250_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBH0067795.1"
                     /translation="MHLFIEVIAAFFTALSFGVLFNMKGKNLILAGIGGSIAWFSYKF
                     LLNMGVTENLCFFIATVCFAIYCELCARIYMTPATTLSVCCLIILVPGYGIYNTMYSV
                     LTNNYIKAVEYGVSTLSCASSIALGLVFITTVFRKVNLYGVLTKIKENEKYKKSINKI
                     KQQK"
     gene            complement(32486..33247)
                     /locus_tag="KR250_001860"
     CDS             complement(32486..33247)
                     /locus_tag="KR250_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBH0067796.1"
                     /translation="MDIDRILNFSSNAGKAMLQSGGETYRVEETIARICQSFGIDHVD
                     VFATPTAVMASVFVDGKLHSAIKRISSRRVDLNLVHEVNSLSRAISINNLDIELCEKI
                     LDEISEDNYYSDLITIFFAGIAAATFSILFGGNVEEFIAAFTVGILTKFVIMYLSKSS
                     LNDFFVNAIGAVIIASCSILILKLGFIKTLDHLIAGAIMLLVPGLALTNALRDLLDGQ
                     LISGLAKLAEVFFVGVSIAVGMFLVLGLYFKLGGI"
     gene            33969..36803
                     /locus_tag="KR250_001861"
     CDS             33969..36803
                     /locus_tag="KR250_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBH0067797.1"
                     /translation="MLRKDKVLLSLHKLCENMTLKDLQSDKTGFTTIEIANHSSLDRS
                     NTSKELNNLYNEKKVLKISGKPILFLHASIIENLIGRTLNSDEFSINNCNELLKSSKN
                     DSEDIFSTLLGHDSSLKLPIEKAKSAILYPPKGLNTLLIGPTGVGKSTFAETMYKYAV
                     ESKIFSSESKFVVFNCAEYAENANLLLSNLFGYVKGSFTGANKDKFGIVAQADGGILF
                     LDEIHRLPPEGQEMLFLLMDKGIYRRLGESDIVHKANVFIVCATTEDIHSSLLGTFLR
                     RIPVTINLPALQDRSLKERLSLIKYFFIIESKYTNATIKVHKDVIKSLLLYDCFGNIG
                     QLKSDIQLMSARAFLNYKTGLKDNIEIDLSLVPDYIYIGLLKLNENRSNLNKLSELDY
                     MLFYEFNGNNLEVEYTKELSKYNIDTSNNGFKYSTEIPYLENDIRSMFINYMEKYSTK
                     LLSISSVTHSDYTSNEIFKVINPKIYYAVEHSLKEAEKISRNKYTKQTYIALSMHISA
                     LIENINQDTYNPKYNLYNDKIFLDKDLNIAKIVLSIIENDLQTSLPKDEVKFISMFLN
                     SMYLEPNNQSESIGVIILAHGNSTASSMCDVANSLLGTNHCNAIDMPLDVKVSTILDK
                     TIELVKECDEGKGVLLLTDMGSLIAFGELISEKTKIKTRSIEMVSTPIVLEAVRKSTL
                     PEMTLDELANSLSSLNPYIGRAAIDSTEINYPEKSREYVIITTCITGYGSAMKVADFI
                     NSSLKNISKYNINLVPHNIESFKMYKNKFSNTKILAVVGSVDFNVANTPFIHIEELLN
                     GYGLDMLNKIIEGTFNANSITNIKETNLSYNSLIINFMTQNLELLDSVKLFNYVNSSL
                     FTIIELIDENLKSKFKLGYILHCSFMIERCFKNTTFPYNNITELISKNHELYSLIREA
                     FKPTEAYFNILIPDEEIAYIIDMINEYLNK"
     gene            36846..38090
                     /locus_tag="KR250_001862"
     CDS             36846..38090
                     /locus_tag="KR250_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBH0067798.1"
                     /translation="MEDEILKGKIKQLTILALIFIFITPVFAFADTPPVPNSSRAALL
                     IDQETKRILFEKNIDEKMPLASLSKMMTFLLAIEAVDKNQVKETDMVKIDKSTASVGG
                     STCKLKDGDEISLGELMQGLMLVSGNDAAIAIAKHIGKTEKNFVNMMNKKAEEIGMID
                     TYYFNPNGLPIYTDPEHKEPPIENMSTAHDIVTLGKYMYDHYENQVTRITTMQVYNDT
                     KKDFTHYNTNPLLVSVPGVDGIKTGYTDNAGYCLAFSMMVPKDAKNERNHRLIGVVLG
                     DGNKKNRISSSATLLKYGKDNFHSKKIAHKGDIIETPCVDGIDDFKITVKVDKDLYGV
                     VSDNENINPKVVFKNMNYPIHKGDIVGVVKYYNDSGKFVGSVDVKSESNIGCIPLKDK
                     IKIKVAKINKKLEIKNSVCFKA"
BASE COUNT        13042 a         6427 c         4494 g        14746 t
ORIGIN      
        1 aatacagtaa attaattggt aagataaatt atttattatt agtaaagtaa attatttact
       61 atttatcaag ataaattatt tataaaataa atcatttata aaatataatt tatactttga
      121 aaataaaata aactatcttc caaacatttt attatatgct taaaagatag ttttgtgtct
      181 ttaaaataaa ttaaattgta tatattcctt cacataactt ttgtatatta ctaggtggtt
      241 gccatttacc atctttttta gtatatgtaa tcatagttgt aacttcctta gttggtttct
      301 tattgtaaag gtctataatc atttttgtca tatcatcttg aacttctttt tcttttttat
      361 cgtaattctt ttcatcagtt acataagatt ttactaaagt gttcgcttca ctcattactt
      421 tactagtatc taaagcttta cttacaactt tcaaagttat cttatcctta ctagaagatt
      481 ccactgttac tgtcttttca acaccttgaa ttaatttaac aattgtttcg tatatctttt
      541 cagcattttc tttaccataa agaggtgtca attgagcaat cattgaaacc ttgtccttgc
      601 tgttataagt ttgaatctgt ttatattcag cttctgttaa tgcatcagaa attccttttt
      661 catcatactt atacatttct aatgcagcta aattagcttc aaaattcgac tttacttttt
      721 cttctgcatt agaacaccct atagttagaa ctaaaactag agccataatc gtagataata
      781 ttaacttttt taccattttc atacaaacat cctccctcaa ttaaaataat aaaaaattta
      841 tttatcaaca ttataacata aatttgtcta actatagaat tattttgaat attttaccac
      901 ttccagtcta ttccaatacc gttttctttt gctttatcgt atattctttt tgctgttacg
      961 acatcttgta cacctatccc aacagtttta aatacaatta tctcatcatc atcttctctt
     1021 ccaggaatag ttccgtttat aacattccct aaatcaccac taaaatcatc tttacttatc
     1081 ttaccttctt ttaatggaat taatatatct cctgactcag ataaaactgc atctgttgat
     1141 tcaaaaaata tcttagacgc tctagttaaa cagtttgggc acaactcttg catatctggc
     1201 atatatgaac ctactgcact tattaaagct ccttttttca ctaagtttcc attaagtact
     1261 ggttgttttg atggagtagc taaaactatc acatctgcgt tatttatggc ttcatcagat
     1321 gattcaactg caactatttt agtattatat ttacttaatt ttaagttcat ttcttctgca
     1381 aatttttctc tactttcctt gtttctacta aaaactctta cctcatccaa atttctagct
     1441 tctatcatgg cttcaagttg tggctcacct tgtcctccta gacctatcaa agctccaatc
     1501 ttagaatctt ttttagccaa tacgtctata gctgctccag aggctgctcc agtacgaatc
     1561 tgagttacat aagtgccatc caatactgca ctaacttctc cagtttctcc atctaataat
     1621 agtactgttg ctggaattac tggtttgcct ttttgagcat ttttaggaaa tacagataca
     1681 atttttattc ccccacagcc taattcttct atgtatcctg gcataaataa catactagca
     1741 tcttgacttg gaacagatat attggttctc aaaggattta cactctttcc ttcacaataa
     1801 attctaaaag cttccttatc agcttcaatt gcatctctca ttgtaaatac tttttttatg
     1861 tcgtcttttt ttaaaagtag catttttata cccccaattt taataagtct ctatgctaaa
     1921 tattatagca taacatttaa tttaatcttc tatttcaaat caaataaatc tacatactct
     1981 ggttttagca tagaacttat caacgcttta tttattgaaa attctggtat tcctgctgca
     2041 tatggagtta tatcatataa atcaaaatat acaactagat tctcatcttg caaataaaag
     2101 ttttgattct cttttatacc tttaaaatta tatattccga tattttcttc atcctttttt
     2161 tcaagttcag ctatttgtct tttaatttct tcatctataa cttctttata tttagtatta
     2221 tccttaaaca agtccattaa ttctaaaaat tttccagttc tcatatccac attatatgct
     2281 atatcttgat aaaatccatg tgctccacca ctataagtat aatatctcac ttttatacta
     2341 agagcagaat cagtattctt tttaagttca aagtctacat ttgcaacaaa cttatttttc
     2401 tcgtcaggat tatccttaag atactgcttt gcttctttat atgatttatt ataaaattca
     2461 aatatgtcat tttttatctt atcatttgta gacctttcaa caattttatt tgaatttgtt
     2521 attacaggca tgtttatagt actttctaaa tactcatcat ttttcactat tgttttactt
     2581 cctattgtac ttactgataa agtttgttta ctatttgtat taccctttgg taatgactgg
     2641 ttactcataa cagtcactgc ttccttatta ctatttaaat taattgaagc atctttcctt
     2701 aaattattat atttatagtc atgtttaatt gccaaaacag ccattatagt caatgttaaa
     2761 acaaatacat atcttaaaac tttagctttt gtatcgactt taaaaaattt catattatca
     2821 ttttcctcct aaatataaat ctctcttatt atatttggag tttctagggt tttttatcca
     2881 tataatttaa taaaagatta atttttattt ataaaaaatt aatctttttg ttttaaaata
     2941 ttaatatgtc ttatcaaaca gtttgaaatt tcaccaagag gtaattgtaa cataacagaa
     3001 cctctatcaa aagctaccat tggcattcca tatactatac aagaattttc atcctgtcca
     3061 attgtaaatg aaccattttc tttcattttt aaaagtccat ttgctccatc ataacccata
     3121 cctgtaagta ttattcctat tgaattttta cctgctactt ctgctactga atcaaacaaa
     3181 acgtctactg atggacagtg accacttatt ctttctccct tttcacaagt tacataatat
     3241 ccttgtttat ctgtacacaa tttcatctgg taacccccag gtgcaattaa cacatttcct
     3301 tgttcgactc tatcattatt cttagcttct cttacattca taatacactg cttgtcaagc
     3361 cgttgtgcat acatataagt aaatatagct ggcatatgtt gaactacaac aatccctggt
     3421 gttgattttg gtaggtcttt tataatttca agtattgctt ctgtaccacc tacagatgcg
     3481 cctatagcaa taaccatatt attaaaacgt tccacattac cgcctccaaa aaatcatata
     3541 tcaaaaatac ctttttatcg tttatatata gcaggtttta catatttgta tttagtacta
     3601 cttctgttga ttacttctgc atgtcctaca aacaaaaaac tatctttttt ggtagcatta
     3661 taaaatttat ttataagttc ttctcttgtt tttaaatcaa aataaatcat aacatttcta
     3721 caaaaaatca aatcaaaatg ttgatatgag aaatcattca ttaaattaaa cggcttaaaa
     3781 attacacttt ttctaatctt atcacaaatt ttaaatgtac catccttgtt gtctataaaa
     3841 tacttcttct tccataactc aggaacattg ttaacatttg attctatgta tatacctctt
     3901 ttagcttttt caagtgagtt agttgaaata tctgtggcta tgattttaat gttccacaac
     3961 tctttattat atccaaaata atcatccaaa gtcatagcta ttgaatacgc ttcttctcca
     4021 ctagaacatc ctgcactcca tatatttatg tttcttattt tattattttc ttcccaaaaa
     4081 ggaagtatac tattctgtat aaactcaaaa tgttgaggtt ctctcataaa aaatgtgtgg
     4141 ttagtactca atctatttat taggtttata gcttcatttc ctgtcttatc tttaaataat
     4201 agattcatat attgactaaa agaactaagt tttttctcta tcattgtatt gtataagcga
     4261 ccttcaatta acgctctttt tttacttaaa tcaataccat attctttttt tacatagttt
     4321 acaagaacta taaattcttc atctgtgagt tttatcatgc aaaaacttcc tcctgctaat
     4381 ttatcagaac taactatatg ctattaatca aaaactttcg aatatctaaa attaatatag
     4441 aattatcttc aactttcgca atctctttta tatctttatt aatattagtg ttgttagaat
     4501 tagtcgtctc tagtaaattt agattttcta tatgaataac atcaacaact ttatctacaa
     4561 taattccaac ttgatactca tcaacctttg taattattat acaagttctc tcattataat
     4621 cagctttagg aagattgtac tttatatgca tattaataac tggtattatt cttcctctca
     4681 aattaataac tccacttata tatgttggaa gacatggtac atacgttatt gattgaaatc
     4741 ttatggtttc aatcacattt tctaattcta ttccataaat aacatcattg acataaaatg
     4801 ttaatacttg tttaataatt tcatctttag ccatacttta aatcccctaa tatatttcta
     4861 taatgctatc tgcatctata attaaactta tacttccatc cccaagtatt gtacatcccg
     4921 ataaaccctt attttttata ttaaatcggt taaaataaac tggaaatggt ttcacaacaa
     4981 cttgctgatt acctattatt tcatcaacaa aaatgcatac tgctttatgc tcattttcaa
     5041 taattatcat attaccattc atcagattgg ttacttttgt ttgaatatta tatattttat
     5101 gtaaccttat aatgggaata catgctccat atattgtaat taactctgtc ttatttcctt
     5161 ctgtaattat ttgatttaag tctgtaagtt taaatgttct tcttatggat gatattggta
     5221 aagtaaatag tgaatctcca actttaaact tcataccctc tactatagaa agagttaatg
     5281 gtatttttat agtaaaggtt gtcccttcat cttgtttact ttttacttca attgaacctc
     5341 caactttttc aatactttta cgaacaatgt ccatgcctac tcctcttcca gaaaattcag
     5401 ttatagattc ttttgtagaa aagcctggag tcataatcaa agaattgacc tcgtcatcag
     5461 tatactcatt actttgatgt ttaagaagac cttgttcctt agcttttgcc aaaatcttat
     5521 ctctcataat gcctctacca tcatctgaaa tacttatcac agcttctccg cctagatttt
     5581 cagcagataa aactatcttt cccacttctt ctttaccttt cgctatacgc tcctcaatag
     5641 gctctattgc atggtccata gcatttctta taaggtgcat aaaaggttct gctaaattat
     5701 caattacact cttatctacc tctgtctctg ctcctatagt tattaactct acatttttat
     5761 ctaatttttt attcatatct cgtacaattc tatgcatttt ttgaaataca ccagcaattt
     5821 gaatcatacg catagacatt actatatctt gcaattcatc tgtaagcttg tgtaattgtc
     5881 ttgttgcctt attgaaatta tctatatcaa cacctgtaga atttacgcta tttacaacca
     5941 ttgattccat aattacaatt tctccaacta tattttgaag taagtccaat ttttctaaat
     6001 ttacactcat aatactctgc ttacttattg gtaaatgttc attttcttca cttagactat
     6061 ctttttccat atcagttttt tcatttaaaa catcttcttg tatctcatct tctgtatagt
     6121 tattttgata acttttactt actttattat tatcttttaa tttattttct actgcatcat
     6181 tgtttgatgt atttttcatt tctatttctt ttttactaat atttatgtat tcttttacat
     6241 agagattatc ttctatagtt ttataaacta ttgccttatc ctctttagct acaaattgta
     6301 tataaaaccc ataactttta atatactctg tagactctgc atttgtttct acatcttttg
     6361 gataaaaatc tataaattca cagtaatctc taagttcaaa tacaagcatg aaagctctca
     6421 cattttccat catgcaacct tgttcaaaga ttatttttac tacatcagag tcttctttgt
     6481 attcaaaatt tgaatcgatt acttcttttt cgtctttact ttctaaattt gcattatcct
     6541 ttgatttcat agaacttgat aattccaaag tactcacata ctcttcaatt ccttcaataa
     6601 aattagagta atcagaagat atacaacctc ttgattgtaa ctcatctata tctcttctta
     6661 taaaatctaa tgattgaaag actaaatcaa ataataggtc attgtctaaa acttctattt
     6721 taccttcacg tatagcataa aacaagtctt ctacactatg tgctaaagca gatatattgt
     6781 ctaaccccat catagatgct gaacctttta tcgtatgcat tattctaaaa gtttcattaa
     6841 tgttgttatg agtaaatact ttctcttttt ctatttgaat cataatttca tctaattgtt
     6901 cgagtaataa acttgtctca aatataaaca tatctaacat ttgtctcata ctcggttcaa
     6961 tataattcac atttatcacc tacgacctac aaataatttt ctattatata tgcatctcat
     7021 aatttaaatc cttgatgatt ctcttatctc actatatgta cctcataatt taaatacaca
     7081 attattctct tacttcaata tatatacatc tcataattta aatacatgat aattctctta
     7141 tttcaatata tatacatctc ataatttaaa tacatgataa ttctcttact tcaatattca
     7201 cctcataatt taaacccatg atgattctct tacttcactt tcctttagat gaaatttttc
     7261 tactaattct tttaaaattt gagcttgagc agacatctct tctgaggctg ctgcactttc
     7321 ctctgctgtt gctgagttca tttggactac tatggaaatt tgctctattc cagataaaat
     7381 ctgtgtaatt gctacttctt gactatgtac agccgaagaa ataccattaa tataattagt
     7441 agttttttct acaccattta ctaccaagtc caatgcattt gcagtctctt tagcaattct
     7501 gacaccattt tctactgttt taatagcttc ttctatgaga atagctgtat tttgagctgc
     7561 ttttgcactt ttaccagcca aagacctaac ttcatctgct acaactgcaa atcctctacc
     7621 atattgacct gctcttgctg cttcaacagc tgcatttaat gcaagaatat tagtttggaa
     7681 tgctatctca tctattgttt tgattatttt tgatatttca ctcgattttt cacttatttc
     7741 acccattgat gtcatcatat catccataca tctacttcca aaaccaactt gtgatgcaga
     7801 ttggtcagat acctcacatg caagactagc actatctgca cacttcttaa ttccatctga
     7861 tatctcatta atcgtggaag ctaattcttc tactgaacta gcttgttcag tagcaccctg
     7921 tgaaagagct tgtgcacctg ctgcaacttg ttctgaagca cttgcaactt gctctgctga
     7981 taaatttatt tcagaaaaat caccattaaa agagtttatt atactttcta aagatatttt
     8041 aattgattta aaatcaccta taaattcttg gtctatctct actcttaaat ctccttttgc
     8101 aatactagtt aatgtactgt ctataaggtc tatgtaattt aaaagcatac tttgcatctt
     8161 tttgacagaa ctcactaata gccctatttc agatttactt tcttcaacgt ctatacttgt
     8221 tcttaagtcc ccagatgcaa ttcttgaaaa gtgtttttct actttattaa taggaatcac
     8281 tatttttaat tttattactg tatattctat tattgtcaat attattaaaa gggaaagtat
     8341 aaatattgtt cttaatatta gactttgtaa ttcttttttt acctctacaa ttttggaatt
     8401 tgaacgggta actatatcct cttgaaattc atttattaaa gaatccattt tctcttggga
     8461 gtttatatag tcactaccaa aaattatact ttgagcttta ctcttatcat tactttcgac
     8521 tgcctttatt gcactttgtt ctgttggtac taaagaatct gatatttctt ttgcttctaa
     8581 gattttattt gactcgctct ttgtaagacc aattttttct aatttgttta aagctgattc
     8641 tcttgttttt ccgtataatt ctttttcata aatatcttta ttcttttttt ctcctgtaaa
     8701 tacatatctt cttaatacat cacttatctc tctagttgta tcttttatat actgactttg
     8761 agtcctcaaa ttattcatat cattaatact tttttctact ttttcaaatg ctttataaga
     8821 tgaaatagaa gaaaaaatcc ctgctagaac agccaatact ataataatca gcaatacaat
     8881 tgctaattta gaaagcttta cttctctttt catatctgta ctccttcata aagtaagttt
     8941 atatcttttt taatcctttc attgtactca ctacgctaca atcttttcct aagtttttac
     9001 cctcatacat tgctttatct gctaaggata tgttttcttc taatgatata tttgagttta
     9061 cttctactac tcctaatgta atagtgcact ttattttatg ttctttatag ttgaaatcaa
     9121 actcctttat ctctttacgg attctttctg aaagtatctt agcaccttct attggagtat
     9181 ttgtgagcat tatcaaaaac tcttctccac cccatcttga tacagtatca atttgtctac
     9241 aattcttttt tattatactt gatattactg ttaacacgaa atcccctgct tcatgcccat
     9301 aagtatcatt gataacctta aagtcatcaa tatcaccaag gataagggat attgatgttt
     9361 cactatattt attaattctt ctttgctcca taagctgatt aattatatgt cgcctattgt
     9421 aaagacctgt aagataatct cttacaacga gtctattcaa tttttcaaga gaagattcca
     9481 gttcattatt tatctttttt aatttatctt gagatttctt tacttcaaga tgagctataa
     9541 cacgagcttt taattctgtc atactaaaag gtttcaccat atagtcaatt gctcctacac
     9601 caaatccttt tactatgtct gtatctgtat tttttgaagt tataaaaatt ataggtatat
     9661 ctgctgtacg agcattagat tttacttcct tacaaaactc aaaaccagag ttatctggta
     9721 aaattatatc aagcaaaata atgtctggta taaattcttc aataaattct tttgcctcta
     9781 ttgcattaaa tgctttttta accttaaaat tatcttttgt aagtgtttta tctattaagt
     9841 cacacatcag aacactatca tcaacgacta aaattgttcc ctgttgctta tttttcatat
     9901 acaaacctct cttaaacata atatattcaa attttaatga tttaaaattt ttatacttaa
     9961 taattatacc actatattct taaatgttct catcacaatc tttttataat aacactccaa
    10021 ctacatccct atgttacatg atagtgcaat caaacccatt gattaattca tctattaaaa
    10081 cttcatcttt atctgtcaaa agtttctcta aatctagtaa agtaattatt ttaccttcta
    10141 attctccaac tccacttaca aaataatccg aatattctat agtaactctt ggtggaggtg
    10201 atattttact tttttctaag cttactatat catcaactct atcaaccaca aatccaacta
    10261 ccaagtcctt tatttcagtt actactatat acatgcaatt tgtagtcatt aattcatctt
    10321 ttttaagtct caaacgcatg tctattatcg gaatgatttt tcctcttaga tttattatgc
    10381 ctttagaata tgtaggtagt tctggaataa atataatttc ttgatttcca gtaatttcta
    10441 ttaccttttc aagttcaacc ccaaagtctt gctcatctac tctaaacttt agatacttaa
    10501 ttccttcatc aattagtcca taatctaaat atgggtcatc tggaaatctc atttaatacc
    10561 tccctcctaa aatacacttt ctatgaaata tatattataa tttcttctta cctttattta
    10621 ttgaatctac aagtaattca cccgtctcag tgtaaaaacg tattgttcta ccataattca
    10681 tacctgtgtc ctcagctaat attggaatat ttaatcctaa aagagttttt tttactgcct
    10741 caacattcct atgtcctata cttgcaatta ctgaattgct acttcctaaa aacatctgag
    10801 cacctccagc aatttttgct tttatatata ttctatttgc acctatattc tccatagatt
    10861 taatgagctc atttattcct gtgtcagcaa atttacactt attttcaatg ttaatagcat
    10921 ctttactata tggcaacata acgtgtaaca ttccaccaat tccagctact ttatctatca
    10981 aacatactcc tacacaagaa ccaagtgcat aagtaactaa aacatttggt ctataggcta
    11041 tcttcatatc tgcaatgttc actactatct ctctattcat acttctaatc ccaacctcac
    11101 gagtatttct ctaagtgagc tcatttctgg aatcatcaaa atgtgactag taaaataatt
    11161 atcactcatt ttaaatttat tttctataaa taagactttg tcaccaacat ctgaaaatct
    11221 tatcataggt acatttagaa cagctccaac catatcaata catatatctg gaattgaaac
    11281 acttatagtc atgtttaaca tagatgcaat agcatttaca tatgaacttg ccataatatt
    11341 tcctatttcc ttaattgctg aaatttcaat tgcctcaatc tcatctaaaa aagcttcttc
    11401 tttattaaaa agagaactta gcataagttg agttattgat tcatccaaca aaaacataaa
    11461 cattccattg atatcacctt tcatatcgag taaaatacat actactttgt tttctggacc
    11521 accaagcaaa tttgtcgctt cattatattc aagtatcttg accataggta ttaatacttc
    11581 tacattgcta tttatcatgg atgcaagtgc agtaattgca ttcccagagc ctatattacc
    11641 aatttctcta agagcatcta tatgaacatc atcaagttct aaataattta taatataaac
    11701 ccctcccttt attattgcat ttatttgctt ttcattgtat tagtgtatat ttacactttt
    11761 attttaaagc attatctaca actttaacta atgtttctgt tttgaagggc ttcactataa
    11821 aatctaacgc tccaagtttt atggcttcta caatcatatt ctcttgtcct attgcagagc
    11881 acattacaac cttagcactt gaatccaact tttttatttc tcttagcacc tctaaaccat
    11941 ctttttttgg cattgtaata tcaagcaaca tcaaatcagg cttttcatat ttaaatatat
    12001 tacacgcttc ttctccatca tttgcttcta ttaaattatt atagccactt ttactcagag
    12061 cttctttaat tatcattctc ataaaaatag catcatctac tatcaaaatt ttattttcca
    12121 taaaactaac tcctccatat gtacaacaat aaacgctttt atcttcagtt attgtctata
    12181 ctatctgact atttttcgct acacaataaa tacatttcat tgataaataa aagtaattat
    12241 ttatgccata ttatacttaa ttctcttaac ataatttgtc aacaaaaaaa cttatatatc
    12301 tattgtaata tatgtttggc gttttgacga gttattaaaa atataattgt ataaaacata
    12361 aaaagttatc aaaagtaatt aaattataat aaaacaactt tatacctttt ttatgtttgt
    12421 ataaaaaaat ttttcagatt cattaaaatc atttctgtag attcatatag gatttgactt
    12481 tctcaatatc taatagatag ttgtttattt acttaattat atttctctat atgtattatc
    12541 gattaaatta gatttagctt tatacattat tttttatatt tttcaaattg tatactcata
    12601 aatccagaag tattctaatg tgtttataat actttttttc atattttaaa ttaaataaca
    12661 aatcattata tttacaagta tcatttatca ataaacaata ttaaatgaag aatatgtctg
    12721 aagattcaaa gtgatactga taatattaat tactaaaaaa gcagtaaatc ttttacagat
    12781 ttactgctta tagttataaa taataaaact atctataata aatgtactat actcaatact
    12841 tcattcaata tagtaataac aaatctactt tattcctata taaatatgaa cttcttgttt
    12901 ctttgtatca aaagtattac attgatactc ttcaaaatca cttatataag tcctatcaaa
    12961 atccatttgc caaaactcaa accaaaaatc tcctacttct ttttgacctc ctactataac
    13021 aaactttgca tacttacctg ctggtataac tttactaata atactttctc caacagtatt
    13081 attagtgttt aattcttcta aattcttctc tttatcagaa ttactattta cttcacaaca
    13141 tgcaacaaag ctatacggac ttgtaaaatc accctggtaa tctgtatata gacctattgt
    13201 cttgtcattc tttttaccct ttatagcatt atatatccct ttgcccataa attttttcca
    13261 caattcacct atatcattta ccgcttgtcc atctttattt gtagtctcct tcgatattcc
    13321 tataactatt ttctcattta tctctactat ttcataattc atactttata cctcactttg
    13381 tttttattaa tatattttat tttgaactaa ttcaattata atttataata tacgacacct
    13441 ttatgtcata tttttaatat aattaaactc agtgagatat tatatactaa atgcaagata
    13501 ttatatattt aataaaatct ttaatctagc attatattat aatgaataaa ttttagacaa
    13561 ctcccttagt ctagttatta cattatccct aacatcttta ggttcaagta cttcaacatt
    13621 gtcaccaaat gataaaatat atccataaat ccattcacca ataggaaatt ctgtaattac
    13681 atcatatgta tcatcttcat ttttagttat taaatcctta ttaaaatcat caaatactct
    13741 aaaaagcatc ttttctttaa acctcaattt caacgttata gtattttcct taattacttt
    13801 agaatcattc aaacatactt cttctattat ctttcttgaa aatacttcat cctttataga
    13861 taaattcctt atcctactaa ttctaaatat cctaaaatca tcttttagtt tacaaaatcc
    13921 atacaagtac caagtcttac tcttatacat aagcttcagt ggttcgacag ttctgtttgt
    13981 ctggcttgac aaactattta cataattaaa atttatagta ttacgcctta aaatagcttc
    14041 ttttattttt gtaaatttat catcttcatt aaaattactt ccccattcag taaagtctat
    14101 ttcaatccag tttgaaaaat tctgctctcc aaatattgag ccaattttat ttactactga
    14161 atttatttct ggatacttag tcgcctgtaa tgttttcagt gccactatta aactctcttt
    14221 atcttttttt gacaatatag ccttgtctat agaatattct tctattaaag atatccctcc
    14281 tcctttgcct ttcgtcatat atactggtac tcccgacata gacagagttt ctatatctct
    14341 atatatggtt cttgtagaaa cttcaaatct atctgccaac tctttagctg ttactaattt
    14401 tttatttaat agaattacta ttatttctgt taatctattt attttcattc attaccacct
    14461 actaaaatta ttaaatttat ttaatctaaa ttaatcatac taatttttaa ataaataaaa
    14521 tcttagaatc ttatctaaat tattcttaca tgattttatt tactttctat tatctttctc
    14581 actttggata ttatcatctt aatttattta ttactatatc aatttactta ttatcatctc
    14641 atttgttatc gtcttactta atttacttat ttctatatca attcacttat tattacctca
    14701 cttgttatca tcttacttaa tttacttatt tctatatcaa tttacttatt atcatctcat
    14761 ttgttatcgt cttacttaat ttacttattg ctatgtcaat tcatttatta ttatctcact
    14821 tgttatcgtc ttatttaatt tacttatttc tatatcaatt cacttattat catcttaatt
    14881 catttgatta ttatcattta ctttattcaa tttctatatc aaaaattagt atttgtactt
    14941 aatacttaaa attaatttaa agtctttaca tattatttat tttttattgt aatattaatt
    15001 tccatacttt gctaagctac ttccactcat ttattttaaa atttcaattt actatttctc
    15061 aattctatat tattattttt caattttata ttactaattt taaagctttt tagctattat
    15121 tcctacaata tttaaacaat tttttaataa taaagctata ttacaatata ttcaaaatat
    15181 gtaatatttt agatattttt cgttgacaat gttaaatttt tgttttatcc ttaattaagg
    15241 acatgttatt gacatttctg aaacttattt gaaatagttt tttcatttta tttaggaaaa
    15301 cgtattatac ctaagaaaac gtattttctc atttcagata agtaaaatat ttattctttt
    15361 ggggaggaaa tcatggaaat taataaaaaa cattttattc aagtattttt aacttgctta
    15421 atcttggtag taatatgtga atttataggg caaagaacta tagctattgg aaaagcctct
    15481 atagtactgt tccctatgct ttatgcagtt ataattggac ttataataac accagattta
    15541 ttaggtaaaa aaattaaagc acttaaaaaa gtggttggag aaaaagaaat aaatattgct
    15601 ggtgaagttg ttggaattgc actagtaatg cttggtataa aatatggtac tacagttgga
    15661 ccaaacattg acaaaataat tcaagctgga ccagcattta tcgctcaaga atttggtcat
    15721 atacttgcac caatcgttgc attacctctt gcattaatgt ttggaatgaa aagagaagct
    15781 gttggagcta ctgcaagtat aagtcgtgaa ccttctttag gagttatatc agaaaaatat
    15841 gggataaact caccagaagg tagtggtgta cttggtacat atttaatggg aacagttctt
    15901 ggtactatat actttagtgt actaggttca atatctattt atagtggtct tcacccatat
    15961 gctttgggaa tggcctgtgg tgttggtagt ggaagtatga tgacagcagc tgctggttca
    16021 ttatctacca tggtaccacc tgaaatggca gatacaatac ttgcctatgc tgcaacaagc
    16081 aatatgatgt ctagtataac aggaattata ttcttagttt ttgtatcctt accatttact
    16141 aattttatgt ataagatttt agaacctaaa ttttcgagaa ataaaaaaac taaagaaatt
    16201 aaatctacag aaggagagtg ctaataaatg tttgctaatt taaatttaaa gaaaagttca
    16261 tatcaattat tggttatgtc tgttattgga gttataattc ttataggaca aagaattagc
    16321 gttggtacat ctatagttac agctcttcct ggtatggtga tgctaatatt agctgctatg
    16381 gcagctatga ttataaaaga tttatttcct aaatcaatct ttccagcttt tggatttgca
    16441 actataatcg gccttttact tagtatgcca tatagtccaa catctgaagt attcttaact
    16501 aacactaata atattaactt tatggctata actactccac tactagcttt tgcaggaatt
    16561 tctgttggaa acaaaataga agctttaaaa gaaatgtcat ggaaaatagt tataatttca
    16621 cttattgtat ttacaactat attctttgca tgtgcgagta tagctcatat agttttaagt
    16681 attcaaggta aaatatagga ggaatttatt atgaataaag aacaattaaa atctctagta
    16741 ataaaaacta tagatgaaaa tagagaaaaa atattaaaaa taggtcaagg aatatacaaa
    16801 aatccagaat atggatataa agaatttaaa actacagaag ctgtttcaaa cttttttgag
    16861 caagaattaa atttatctgt agaaaaaaat atagcagtta caggatgcaa agcagatgca
    16921 aattctagca agaaaggtcc tcatatatct atacttggag aattggatgg tatatcatgt
    16981 aaagaacata aagatgctaa tgaaataggt gcttctcata cttgtggaca taatattcaa
    17041 atagctggaa tgttaggtgc tgctgttggt ttagtaaaaa gtggcgtttt agaacattta
    17101 gatggtaaag tatcttttat ggctactcct gctgaagagt ttattgagtt ggaatacaga
    17161 gagcaattaa aaaaagaagg aaaaataact tattttggtg gaaaacaaga attaataaaa
    17221 agaggttatt ttgatgatat agacatatct atgatgtttc atgcttctga tttaggagaa
    17281 gataaggctc tagttggtcc tgagagtaat ggatttgttg gaaaaaaggt taaatttata
    17341 ggtaaagaat ctcatgctgg ttctgctcca catgatggaa taaatgcttt aaatgcagca
    17401 atgcttgcta taaacaacgt gaatgcactt agagaaactt ttaaagagtc tgaaagagtt
    17461 agattccacc ctatcatcac aaaaggtgga gatatagtaa atgttgttcc tgctgatgtt
    17521 catatggaat catatgttag agctagaact ataaatggta tgattgatgc taatgaaaga
    17581 ataaataagg ctttgatggc tggtggaatg gcagttggag ctgatgttga gataactgaa
    17641 attcctggat atcttccaat attacgatac aaaagtcttg ataatttatt taaaaacaat
    17701 cttgaagatt taggtctaaa aggaaaagtt attgatggtg gagattttac aggctctttt
    17761 gattttggag atgtgtctca tataatgcct actcttcacc ctatgattgg tggtgtaaaa
    17821 ggagctcttc atacaagaga ttttgaaata gttgatgaag atttagctta cataattcct
    17881 gctaaagcaa tggcacttac agttgtagac cttctttttg ataatgctaa ggatgctaaa
    17941 gatatattag atacctttac tccagttatg actaaagaag aatatctaac attcttagaa
    18001 aaacacgata aaactttata agtctatttt tataataggg tggtatctac tgtaaaagtt
    18061 ataccactct ttgtttttgt aacagtatac ttcttttctt ttagttttta gattaaacta
    18121 agatatatta tcctccttaa aacttttaga gctacgttga aatatcagta gaactatgct
    18181 atattctttc taattaatat aaatttctat tctaattcaa ggtggtgaaa aaatgtttaa
    18241 tgcaggaaat cgaattataa atacatggtt gtaccctatt caagatggct ttgtcttgat
    18301 tgatactgga tatgaaaatg gttttaaaca tttcaaaaaa cgaatttcta aattcaacat
    18361 aaaaatcaaa gatattcgat acatatttct aactcatgct catgatgacc atgttggttt
    18421 tctaaatcag attctagacg aatctcctaa tacaaagatt gttatgcatg ataaagcttt
    18481 agaaattcta tataaaggtc aaaactcttt tattggtggt tgtaccagcc gtactgcctt
    18541 tctcttttgc caaattatga aactatttgg aaaaggtgag catagttttt ctccacttaa
    18601 acaagaattt gaaaaaaggt gtattcttgt gtctgacaag aatcgtaaag aaattgaaaa
    18661 agaactaaat ggaaaaatta tagatacacc aggacatact gctgattcaa tttcactact
    18721 aattgatgaa ggtgttttat tttgtggaga ttctgcaatg aatggatttc caagtactca
    18781 caaaattacc atatttgcag aaagcaaact agaatttatt caatcttgga aaacaatcat
    18841 caatacaaaa cctaaaatga tttatcctgg acatggaaaa ccatttgaat acagtaaatt
    18901 agaacaaaat ctaatctatg taaataaaat aaatttatat cctctacata attcaaaaga
    18961 tattcacagg tttttgtaga aaatacaaag taaatttatt tgattaatta aaaaatctaa
    19021 cactttacta cgctacttat tttaataact taatcttcca ctagcttttc ataaaatttt
    19081 gttttaaaat taattttaaa gcgcatatgt tatttaaata cattagataa aattcgtcat
    19141 taatctactt aaagcatatt taactaacac cactcaacca gaaaattcag aaaactgcat
    19201 tgacacagga aaaagtttat tttatacttt ttttaggaca tattgtggac acgtcttaaa
    19261 tcgacaaaca gaatttaaat tgtaagatta accacaatac ttatttttta attatatttt
    19321 taaacattag ggaggatatt atgaatgata acgtagttaa gaagaaaaac ttttttgaca
    19381 ggacattaaa cagaatagaa actgttggta ataaactacc agacccagtt acaatatttt
    19441 tagggttatg tgtactttta cttatactat ctagtctagt tggttctatg ggtatatcag
    19501 tagttcatcc tggtactgga gaaactataa ccgctgtaaa tttattgact gtagaacagc
    19561 ttcaaattct tttaggaaac atagtcagca atttccaagg gtttgctcct ttgggtctag
    19621 ttctagtaac tatgataggt gctggtgtat gtgacaaaac aggacttatg acagcaacaa
    19681 tcaaagcatc agtaagcaag attcctgaaa caagagtaac tcttgttgta atgactatag
    19741 gtatgcttgc caatatagct tcagatgcag gtacaatatt atttccacca ttagctgcac
    19801 ttgtatatct tggtgttggt agacatccac ttataggttt attttctgga tatgcagcag
    19861 tctgtctagg atttgccgca aacattatga taagtgtcaa tgatatactt gctgcaagtt
    19921 ttacagttcc agcagctcaa atgattgatt ctggatttca agccaatgca acaatgaact
    19981 tattttttat gatagcatca acttttgttc taatactttt ggcaacatgg gtaacagaaa
    20041 aaattgtagc tcctagattt ggtaaatatg aaggaaatgc agaactagat gttgatggaa
    20101 gtcttagtga tgtagaaaag aaagggctta aaaaagctgg tataagtata ttaatatatg
    20161 tggctattat agtagctctt agtgtaatcg gtaaaaaacc attcttagct gacccagaaa
    20221 caggagattt actatcaaac aatgctcctc ttatgaaagg tatggtacct ataatagcac
    20281 tagcattttt catacctggt gttgtatacg gaaaaactat aggtaaaata aagaaggata
    20341 aggatgtagt ctctataatg gctagtgcaa tgagtgatat gggtggatat ataatactag
    20401 catttgctgc ttcacaattt ttgcaattat tcacaaatag taatttaggt ctgatactag
    20461 ctgtaaaggg cggagaattt ttaaaatcag caggaataaa tggaccttta ttgttgatag
    20521 gatttattat attatcatgt tttataaatt tatttatagg tagtgcatct gcaaagtggg
    20581 ctattcttgc acctatattt gtaccaatgt ttatgatggt tggatacaat ccagcactta
    20641 ctcagatggc ttatcgtata ggtgattgta taacaaatcc acttagccca ttatttccgt
    20701 acttccctat tatactagca ttcacaagaa aatacgataa agatgctggt atgggtactg
    20761 taattgctaa tatgatacca tactctatat cattcttaat agtgtggact atactattaa
    20821 tattgtttat ggtatttaat attccactag gacctggaat acttccttca tatagcatgt
    20881 aatttattta aatctttcta aaaggagaaa taatttatgt tagataaaaa tagacttata
    20941 aataatttta tggatatggt tagaattgat agcccatcta atcaagaatt agaaatgtct
    21001 aagtggttgg taaactactt aaaagaaaga aatatagacg ccattataga tgatgcaggt
    21061 gaaaaatatg gtggtaatac aggtaatgtc attgcctata taaaaggtga agaaggaagt
    21121 agaccacttt gtttatgtgc acacatggac caagttcaac cttgtttagg tgtaaaacca
    21181 atactagatg gtaatgtagt tagaagtgat ggaactacta cacttggtgc agatgataaa
    21241 gctggaatag ctgctatact tgaagcttta gaacatgtta ttacagaaaa aataccacat
    21301 agagatattt atctttgttt tacaatctgt gaggaagccg gtatgcatgg tgtcaaaaac
    21361 tttaatcctg acaacttgcc ttgtaaagac atggtgattt tagattctgg tggagctatt
    21421 ggttcgatag catacaaagc tcctgctcaa caatctataa aaatctcttt tcatggaaag
    21481 aaagcacatg ctggtataga acctgaaaaa gggttaaatg ctatattagt agcctctcat
    21541 gcaataagca acatgcacat aggcagaata gattcattga ctacttcaaa tataggtaaa
    21601 atagaaggtg gtggagctac aaacatagta actgataagg ttacattgac tgctgaaatt
    21661 aggtctcata tacctgaaac tttagagtat gagcttaatc atatggaaaa atgttgtaag
    21721 gatgctgcat ctaaatttaa tactacttat acttttgaac atagtatgtc ttaccctagt
    21781 tttgaactta gtagagatag tcatgttttc aagttaagtg aagaagcaat aagacaagtt
    21841 ggtgttgttc caaatccaat ggtaattggt ggtggaagtg acgcaaatat attagctaat
    21901 cttggatata attgtgctat attaagtcta ggtatgtatg atgtacacac agttaatgaa
    21961 tatgtaaata tagacgaatt atatgatact gccaaaatag tttatcatat gattaaatta
    22021 taaatatcat aatataactc tttaaattat aaaaataaaa acctatgtat cttcaacttt
    22081 ataagttata tattaaagtt gttttacata ggttttagta ttttatataa taatttgttt
    22141 taacattttt cataataagc ttcaatattt tgcataataa tttgttctag catttttaat
    22201 aacaagcttt agtattttat attaataatt tattctagta ttttacataa tcatttgttc
    22261 taattttttt cataataagc tttaatactt tatatagtaa tttgttgtag aattttatac
    22321 aatataatga tttatacagt tttataaaat atcttaaaac agtatttcag taatattttt
    22381 tggtcttcct ccaccaatac tagtctcttt tgcacatatt cgacctaaac ctgcatttac
    22441 tattttattt aatatacgtc ttgcacttct ttcacttaca tttagatact ctgctaactc
    22501 ttttgaatca taaatcttac tttttctatt ctcatttata gcaataattt ttgcaactga
    22561 ctcacaactc attccagtct ttttagatat ttcgattacc ttttcatcag atagcatcaa
    22621 agaataattt aattcatttt cggaagctaa aggtcctctt atgttttcat cttcatctac
    22681 caaatatata ccaatttctt ttgaatctat ggagtgtctt aatgctttat gtgcattatt
    22741 ttcagcctga tacgcagtta ctccagtacc aattcctata ctaagagaaa agcccatgtc
    22801 ttttatctct ctctttagat tgaatagctt atcataatta tactcattat cagctacccc
    22861 tttatgaaca aatacaacat aatcatttct tccaagtaaa aataaggaac cttgaatact
    22921 tcttacatag tttactacaa gcttatccat atctgatttt tttatcatgt cagaataata
    22981 gttatctatc ttctctttat aatctattaa actcaatatt tcaacagcaa tttgagaaaa
    23041 tcttgcctta cttagagcat attcactctt taccttgtca taactttctt tgattaattg
    23101 tatagtagct tgtaaacgaa atacaggata gcctctttct tttaattcat tatatactgc
    23161 accaaaacca gttaacatta tatccgtctt attttcttca aataatttta tatgccattc
    23221 tacaagttca gactctccta cttccaaaga aaatggatta caaaatacac tcgttgcttt
    23281 aatatcaaat tctttaagtg catcttccac aacatatcta tcaactacat ctatactgaa
    23341 tttatctaat tttatatttg cactttttat tgcccaaaat gccttcatta tactcgtccc
    23401 acttctcggt acaaatgaat gtggcttact tatctcatac ttagatttta taaattcata
    23461 aactgcacat cctgtaaata taatagcatc acattcgtct tcacatttag aaataacttc
    23521 tattgtatct actacttttt ctcttacata tattttagtc tttaaatcac tgtctatctg
    23581 ttttaaatca tcctttatct taaacccaga gtctgaagga cctataatac ccactatcat
    23641 aaacttcgct cctaatccat aaatattata ctttttaaaa attatatcct cactattttc
    23701 tacttctatt taattatttt gaactactcc atcctaccta catttagaat ggaataatta
    23761 gcttattttt gataaagtaa cttctcaaaa tcagaacttg gaataggctt acaataataa
    23821 taaccttgaa ttaccacaca atctaaactc actaaaaatt cagcttgttc ttttgtctct
    23881 acaccttctg ccactacatc catatctaaa tcttttgcca tggctatagt gtgctttaaa
    23941 actatttttt ctctgtctgt gattccttcc tcattaaaga aaccacaatc tatctttaat
    24001 gtatcaatat taatattttt taacatattt aatgaagaat atgctgaacc aaagtcatcc
    24061 atatttaaaa caataccatc cattctaagg tcggctatta tcttagtaaa taattttata
    24121 tttttaataa ataaactttc tgtaagctct aattctaaaa aagattttgg tatactatat
    24181 ttatttatta aacttaaaac aatttcttta aaatgtggat tatgcaaatg taatcttgat
    24241 acatttacag agataggaac tggctcttca cctaaatcta tccattttct aatcaaagaa
    24301 catacttgtt cccatacata catatctaat ctgataataa acccattctt ctcaaatagt
    24361 gaaataaatt taattggagg tattatacct ttttctggat gaacccatct aaccaatgct
    24421 tctgcaccaa caattttatt tgcattaaga ttatacttag gttgtaaata tacatggaat
    24481 tgcccatttt caagtgcgta ttccatttcc ctttccatct gtttttcttc aagtgcatca
    24541 tttcttatag tatcatcata aaatatatag ttgttgatac tttgtttctt tacttttttg
    24601 tgagcatatc ctgccatttc acacgcattt gtaattggaa tatgattatc caaaattcta
    24661 taaaccccaa aacatggtac taacatatat tcaaattttt ttctatttaa gctattttta
    24721 atcttataaa ttactttatg aatatctcta tcagagtaac tctcataaaa aatgcaaaat
    24781 acatctgcat acatacgaca acatatacca ttagaaccaa tgtttttttg aatcaactct
    24841 gcaacatata ttaaagcttt atctcctcct ctaactccat aaatgtcatt aattaccttg
    24901 aacttatcta tatccatcat tataattgca taacgcttgt caggatatct tttaagcatt
    24961 aaacttattt gttcacaaaa actttcataa ttgtatagtc ctgttaatgt atctttttgg
    25021 tttttatgta gttgcataag ataatctata ttttttatag caactaaaat atgtgtttgt
    25081 ttctcaccta acttattttt actactaatc tttaacatat aaattaatgc ccaatagtac
    25141 tttttatcta ttcctaaacg tctatactca aaatttagtt ctaaatcgtc ttgcttgcta
    25201 ttattaagac tatcaagaga ttttagaagg tggttatcat taaataatat tatatcatca
    25261 ggatgaatta aatatttttt tatgtatgaa aaagtttttt caagattttc ctttatagga
    25321 aaactaaaaa tattgctctc tgattgataa attggagtaa ttaaattcgt atcaatgcaa
    25381 atagataaaa ttatatcata tgtattataa gctaattttt cataagcttt agaataccct
    25441 tttatcatta aatcatcaaa ataaacctta tcaactaact ctttatgttt aaataaaata
    25501 ttattcgtcg aatcagctaa gtcactaatt actttcattt cattatttct aagtttgttc
    25561 ttattataat acctcttctt atcttggtac attctctcat cagcaacttt aattagctta
    25621 tgtatatcaa tattatcgtc actccaaata ctgcccactg ctacactata tatcatctca
    25681 tccttaaatt cttcacttaa ccaactcact aattcttcaa atagttgttt tgatatatct
    25741 tcacataata caacaaactc atctcctcca atacggtaaa cattataaga atgaaagtat
    25801 ttttttatta ttacacatgc tgacacaata gcagaatcgc cattagcatg tccaaagtta
    25861 tcattaatat cttttaaccc atttatatct acaaatacaa ctcctatgga tttaactttt
    25921 cctttttgaa aattatctat atacttaata tatttatttc tattatatgc accagtcaag
    25981 aagtcatagt agctcatata atttaattta ttttctactt ttcttttttt catctcagtt
    26041 aataaaaaat aagataggga tgtcaaaaga gaggtatctc caacagcttt atgtggatta
    26101 tcaactccaa taaaccctac tactttatca tcttgtttta gtgaagatgt cattaaactt
    26161 tcaatttttt gaggttttaa aatttcatat tctattggct gttcttcttt tatattttct
    26221 atatcatcaa ttattacaaa acctttttgt tcgaataata aaaaccaatt ctttaattca
    26281 ttaatatcaa gatttgttag tgcctgtttt tgttcatcaa caccttcatt gcaccattca
    26341 tatgtattag ttacttttgt tctatcttct gatacttcaa aaacatatgc tctatcagct
    26401 ttatgaaatt taccaatatt ggttaataca agatttacag catcagaaaa attagattct
    26461 gttataagat gtttaataca tccaactaat aactccttta tctctaattt ttctgctatt
    26521 gacttactta tatcttcctt ttctgtaata tcaagagcta tttctaatcg tgcatgtttg
    26581 ccctcccatt taattaactt gtcctttaat ataaaatgtc tatttgttcg agtattcgta
    26641 ttttcccata tgtaatattc atcttctttt aaaatagagt ttgtacaaaa ttcgcaggga
    26701 gaatctttcc cctgtaatgc tttataacat tttaaatggg atatatcatt aagtcccatt
    26761 gcctttttcc caatttcatt aatatataaa agttcatatg tatctaaaga agaaacatat
    26821 acaatttcat ccaactcatc tattaaattt attacatcca tttatatata tctcctttta
    26881 ttcattttcc agattgaatt tttaaagctt ttaaaatttt gctatatatt tggcaatgat
    26941 attatacttc tttatgtttt aaaatagtac cttaatgagg ttctattttt tagtaaatag
    27001 aattattatt gtgtcaatcg tacaatagag ttttgtaata cttaatttaa tatatttata
    27061 attaactatc ttattatgta tttacaatta attctcatat aaatgacaaa tctccacctc
    27121 aattaagtat tccaatcata tatgaggata atacttcatt catttaaaaa tataaaacta
    27181 ttatatatta aaatttcttc aataataaat tatttagatt gttactttga tatattatat
    27241 aatatcacaa aggctgatat aaaaagacaa aaagggcaat ttttttatat caaaaaaaat
    27301 gttaaattga tattatccat atataaattg atattatcca tataggtgct atatttaaca
    27361 attctactta cttcccttac acttattagc atatagttat taagagtttg ttgcaaaaca
    27421 acacttagca cactttaaaa agtgtggtct aataagtaat caacgaagaa gacataataa
    27481 agcataaaat aggatatact tttccacgtc atcaaaattg aggattttat tattaatata
    27541 tgataaaata tgcttaataa attaaatttt aatgggagaa tagttatgaa aatatttagt
    27601 ttcttgttgt ttgtagtggg aatatgtttt tgtattgctg gatttatggg gcattcctca
    27661 gctttttctg gtgcaacatt gagttttttt gcaactacaa tgatagctgt aagacaagtc
    27721 aaaactaaga aaaaatgaaa taggcaaatc ctaactttat aagttaatat tacataagta
    27781 attagtctaa aattagatac tttttctatt acaaaaatac atcatccact ttgtattctc
    27841 ttaaaaaaaa taaaatagct atgtaaattc aagattagat agactaactc tctatccatt
    27901 tcattgtaaa atatacatag ctatttgtat tcgatttaaa atacttagat tttcttacat
    27961 aacaaataat ttctatctga ttattactca tgttcattta ttaaccactc agcaactaat
    28021 catttagtat ctattcatcc gccatacggt aacctacccc aacctcagtc aaaatatatt
    28081 ctggctgagc aggattcttt tcaattttcc ttcttatact tgccataaaa actctaagcg
    28141 actgattttc actttcatag taatttcccc aaactttatc tattataaac ttatgggtta
    28201 agactcttcc tgaatactta gacatcaact caataatctt atattcgatt ggagttaaat
    28261 gtatttcttc accattaatt gacacaatat gattttcata atctatcttt aggtttttaa
    28321 cttcaaaaga ctttggagca tcattattta tattatcctg ttgagcttta tttctaagag
    28381 ccacacgtac tctagccaaa agctctgtta cactaaaagg tttagttaaa taatcgtctg
    28441 ctcctccatc taaagcttcc accttatctc tctcatgctc tctcgctgaa acaacaatta
    28501 ttttattagt ctttgcacat gccctaactc gtcctattac ctcaatccca tcaatatctg
    28561 gtagtcctaa atccaaaatt atcaaatctg ggtcaagaga atgtataagt gatatagctg
    28621 ttcctccatt atctgtctct acacacctat aattttgagt ttcaagagat acttttataa
    28681 atttacaaat tggcttatca tcttctacaa ttagaactaa tggctttatt tccacttgtc
    28741 tttcccctcc tcatcataca taggtatgct aaatctaaat gtagctcctc cagaaggatt
    28801 attaaaggcg acaatttctc caccatgagc ttctactata gatttacata tctccaatcc
    28861 aagaccaata cctcttcttt caagatttct agacttattc ttagtataaa acctattaaa
    28921 aatattcccg atatcttcat ttggtattcc ttttccatta tctgcaactt caaatagtac
    28981 ttgatttgac tcttttttta cagttagttc tatcttagaa tttttaggtg tatgccttat
    29041 agcattatca attagattta ctaacacttg ttctattaat aatacatcca catgaacgat
    29101 tattatctca tctggaatat ttgtttttat gtctctacta ttggcaaact ttttgactct
    29161 taatatagat tcagctatta tttcatcaac aacctctggt cgtttttcaa tatccaattt
    29221 tccttcatct attctggtca tactaagtat attctcaaca gaatgagtta gccaactcgt
    29281 atcttcatat atatttttca ataattctac tctagtttct ttatctatca catcatcatt
    29341 ttcaagtatt gttgaagatg aacccaatat acttgtaagt ggagttctta aatcatgaga
    29401 aattgaccta agcaagtttc ctcttaacct ctctctctct gcctcaaggt ttgctttttt
    29461 atttttctca aataaatttt ctctatcaat tgccaacgca acctgtgttg aaactgcttc
    29521 taatagtatc ttttcatttt ctgtaataat atctatctta aaacacgcaa cacctataac
    29581 accaagaaca tttgattctt gaccttttat agggaaataa tatgcactgc tgtttttaca
    29641 atattttgtc ccaattccaa ctgggttacc tgtcctaaaa gcttctttta tagcttcctt
    29701 ttctaatact gaatggaata tattctcact tccatcattg ttacaaatat aaatacttgg
    29761 cttacctaga ttattattct catctgcaat agtcactatt acagttctat ttaatatatt
    29821 tactaaatta gacccacaaa attcaataat ttgattttta ttttttgctt taagcaacgt
    29881 tttgttattc tcatagagca atcttatcat tttttctctc ttatgagaaa gtctcacttc
    29941 tctttttatc ctagaagtta aagtacttgt tataattgct gccattagca taatacaaaa
    30001 ggtcatagga taatcttttc tataagctaa aaaagtatat aatggatgcg taaagaaaaa
    30061 attaaatgat agcactccta ttactgaaga aagtattcca aaaaaatatc cataggttat
    30121 acttgtagca aatagtacac ctaatatgta tactagtata atattagatt cattaaaacc
    30181 taccttttca aaaactactg atattccagt agcaattgtc ataaccaata tagtttttat
    30241 gaagtcctta ataattatct ttttactgag atagccatct tcatcataat catactcttt
    30301 taagtcttca tgttgtagtc gttccttttt catttactac aattcctcct tattgttact
    30361 gtttaaattg gctctatttt atataataca attttatcat aataaattca atcaatacat
    30421 ttaattaatg tacttaaatt actaaaaggt gctgtcttaa aatgaaaata aatctctatt
    30481 tcaaaacagc acctatatat ttaaattact aatttagttt ttacatactt aaatcaatag
    30541 ttttattcaa tttcataaat tataatactt caattataaa acatttctat ccaacaagca
    30601 taaatataaa gtgggcacat atacctgcac ataaaccacc tatagtatgc gaaagtatcg
    30661 ctttaccagc caatggtctt gcatctaaag catccatcat acctatgtgt gtacttaaat
    30721 atccactcca acacataccc attgctgtga atactgctat atcattagga gttatagcgc
    30781 ctgattttat aaactctggt actaaagata ttgctgctcc aacagctcct aatgcagtta
    30841 cagggaatgc tattgcctct ggtgatgtga atccaaataa tggttctatt atgaagctaa
    30901 tcttatcacc aatagcaggt aataatttta taccttcata tgcagctcct gtgtaaacag
    30961 cttgacctgt agcagggtct gtagatggtc caaaagttaa taacataact aatgtacaaa
    31021 ctactaaaac tcctggtatt atagccatac ccatttcaac acctaactta ccaccttcaa
    31081 gtatagcatc caatgttctt tggaatacat taccatccct tatttctcta aactcttcaa
    31141 gttttgcacc tttatcattt atttgctttt cagtatcatt tttagggtca taattataat
    31201 actttttagt aaaagtcaac ataattctaa cactaataat actacctatt attgccccaa
    31261 cattacctat tatagctggt agtacatatt ctttaccttg agctatcata aaagttgtta
    31321 caataagtcc cataccaaag gctgttccta aattacataa agcaggtact tgatacttct
    31381 taaaatattg tgtaaatgtt ttgtctttag caaatggtat aatagctgga ttatcagata
    31441 aatatgttgc aactactcct gctatactag caccaggtaa gccccatata ggtctcataa
    31501 gacccttaaa tatcttatta actaaagata ttactccaaa ctctgaaagc aatgcactaa
    31561 gtgcacctgc aagaactgcc atagccatta ttaaaaatac tgtgtctaat aaaagcgcat
    31621 gtgctgtact cattattact ttaaacatca ttcctgcacc catgatgctt cctacatatc
    31681 caaatcctac cgccaatagt acaataaata cgaaggtttc catacttact gccttgactc
    31741 ttctcttatc cgacagcttt tccatcttga aatcttcccc cttttaattt taaattaagg
    31801 ttcctaacat tggtacaaat tttttggtac aaactgagtt ctaaaactta cactcgtaat
    31861 gcaagttgtc agttttttga aataaaaaaa aattacgttt atagaataag aatatcctct
    31921 ttaatttgga tactcttatt ctattttatg ttattatatt atcatccttt tgtttaaaag
    31981 tcaatttttt ctatttttgt tgtttaattt tatttatact ctttttatat ttttcatttt
    32041 cttttatctt tgtaagtact ccatataagt tgacttttct aaatacagta gttataaata
    32101 ccaatcctaa tgcaattgaa cttgcacacg ataatgtact caccccatat tctacagctt
    32161 ttatataatt attggtcaaa acagaataca tagtattata tattccataa cctggcacta
    32221 atattattaa acagcaaaca cttaatgtag ttgctggagt catataaatt cttgcacata
    32281 actcacaata tatcgcaaaa caaactgtag ctataaaaaa acataagttt tctgttactc
    32341 ccatatttaa aagaaattta tacgaaaacc aagctatact tccaccgatt cctgctagta
    32401 ttaagttttt tcctttcata ttaaatagca ctccaaagct taaggcagta aaaaaagctg
    32461 ctattacttc tataaacaaa tgcacttata tcccccctaa cttgaagtac aatccaagta
    32521 ccaagaacat tcctactgct atagatacac ctacaaaaaa tacttctgct aattttgcaa
    32581 gaccagaaat caactgtcca tctaacaaat ctcttaatgc attagtcaaa gcaagcccgg
    32641 gaactaagag cattattgct cctgctatta aatggtctaa cgtctttatg aatccgagct
    32701 ttaaaattaa aattgaacaa gaagctataa taactgctcc aattgcattt acaaaaaagt
    32761 cattaaggga agatttactt aaatacataa taacaaactt agtcagaatc ccaacagtaa
    32821 atgcagctat aaattcttcc acatttcctc caaataatat cgaaaaagtt gctgctgcaa
    32881 tacctgcaaa aaatattgtt attaaatccg agtaatagtt atcttcactt atttcatcta
    32941 aaattttctc acataattca atatctaaat tatttatact tattgctctt gataatgagt
    33001 taacttcatg aaccaagttt aggtctactc ttcttgagct tattctttta attgctgaat
    33061 gtaatttccc atctacaaat acagatgcca ttacagctgt tggagttgca aatacatcta
    33121 cgtggtctat tccaaaagat tgacatattc tagctatcgt ttcttcaact ctataggttt
    33181 ctcctccact ttgcaacata gctttacctg catttgagga aaagtttaaa attctatcta
    33241 tatccacaaa acatatctcc cattctttta tagtattatt catattttaa tacaagatat
    33301 tatttattta aactatttta aataattcat atccaacatt tacattttat gaaaaaactt
    33361 gttttttatg cttgtcaaaa tcaaatttta agcttgttta caccataaaa tggctgaatt
    33421 aatatttgac aaacactttc aaccatttat ataaccttct aaattgtata actttttact
    33481 acaaataaaa ttatatctct tttaattctt tctcttgagt atttagctta tagtaacttc
    33541 atatttatat atgtacaata taaaacatta tttttatgat acaaaatata atttgtaaat
    33601 gtatatatgt attaactgtg tatatataaa ttattcttaa ccttatcagt tttttcatat
    33661 atcttcatct ataccgacca tatattaatt acattgacca tatattaata aatgatatta
    33721 catagactta agtatattaa aaaacttaaa tttagttatt taacaacata atactctaat
    33781 ttaatctata aaaatccata cctaatataa tgcaactctt attttaacac catctattac
    33841 acaaaacaac atattaaaaa gtaaaataca aaatatctac tataaacctt attttaatac
    33901 tgaatatata ttataatata tttaatgtag cttaaatata gtttaaacat aaagaaagga
    33961 aacggtatat gctaagaaaa gataaagttc tattatctct acataaatta tgtgaaaata
    34021 tgactttaaa agaccttcaa tcagataaaa caggattcac tacaatagaa attgctaacc
    34081 attcatctct tgatagaagt aataccagta aagaattaaa taacctatac aatgaaaaaa
    34141 aagttctgaa aatatctggt aagccaattt tatttctaca tgctagtatt atagaaaatt
    34201 taattggccg tactttgaac agtgatgaat tttctataaa taattgtaat gagctactta
    34261 aatcaagtaa aaatgactca gaagatattt tttcaacatt acttggacat gattctagtc
    34321 ttaaactacc tatagaaaaa gctaaatctg ctatactcta tcctccaaaa ggtcttaata
    34381 cattactaat aggacctact ggtgttggaa aaagtacatt tgctgaaaca atgtacaaat
    34441 atgctgtaga aagtaaaatc ttttcttctg aaagcaaatt cgtagtattt aactgtgctg
    34501 aatatgcaga aaatgctaac ttattattaa gtaatttatt tggttatgtc aaaggttctt
    34561 ttactggtgc taataaggat aaattcggca tagttgcaca ggctgatgga ggcatattat
    34621 tcttagatga aatacacaga ttaccaccag aaggtcaaga gatgcttttt cttttgatgg
    34681 acaaaggcat atacagacga ttaggagaat ctgacattgt gcataaagct aatgtcttta
    34741 ttgtgtgtgc cacaacagaa gatatacatt cttctctgct tggaactttt ttaagaagaa
    34801 tccctgttac aatcaaccta cctgctttac aggataggtc tttaaaagaa agattatctc
    34861 ttattaaata cttctttatt atagaatcaa aatacaccaa tgcaactata aaagttcaca
    34921 aagatgtaat taaatcatta ctcctttatg attgttttgg taatataggt cagcttaaat
    34981 ctgacataca acttatgagt gcaagagcct tcttaaatta caaaactgga cttaaggata
    35041 atatagaaat agatttatcc cttgttcctg attacatcta tattggtctt ttaaaattga
    35101 atgaaaatcg tagtaatcta aataaattat cagaacttga ttatatgctt ttttatgaat
    35161 ttaatggtaa taacttagaa gttgaatata caaaagaact atctaaatac aatatagata
    35221 catcaaacaa tggatttaaa tactctacag agattcctta tctggaaaat gatataagga
    35281 gtatgtttat aaattacatg gaaaagtact ctacaaaatt actttcgata tcttcagtaa
    35341 ctcattctga ttatacaagt aatgagattt ttaaagtaat caatccaaag atttactatg
    35401 ctgttgaaca ttctttaaaa gaggctgaaa aaatatcgag aaacaaatac acaaaacaaa
    35461 cttatattgc tctttcaatg catataagtg cacttataga aaatataaat caagacacat
    35521 ataatcctaa gtacaattta tataatgata aaattttctt agataaagat ttaaatatag
    35581 ctaaaatagt tttaagtata attgaaaatg acttacaaac atctttacct aaagatgaag
    35641 taaaatttat atccatgttt ctaaatagca tgtatcttga accaaacaat caatctgaaa
    35701 gtataggagt cataatactt gctcatggaa attcaacagc aagcagtatg tgtgatgtag
    35761 caaattcatt gcttggtaca aaccattgta atgcaattga catgcctctt gatgtaaaag
    35821 taagtaccat acttgataaa actatagagt tggtaaaaga atgtgacgaa ggtaagggtg
    35881 tacttctttt aacagatatg ggctctctta tagcttttgg agaacttatt tcagaaaaaa
    35941 caaaaatcaa aactcgctca attgaaatgg tatctactcc aatagtttta gaagcagtaa
    36001 gaaaaagtac actaccagaa atgactttgg atgaattagc taattcctta agctctttaa
    36061 atccatatat tggacgagca gcaatcgatt caacagaaat aaactatcct gaaaagtcaa
    36121 gagagtatgt aataataact acttgtataa caggatatgg ttcagctatg aaagtagcag
    36181 actttataaa ttcttcactt aaaaatatta gtaaatacaa tattaattta gttcctcata
    36241 atatagaaag ttttaaaatg tataagaata aatttagtaa tactaaaatt ctggcagttg
    36301 ttggaagtgt agattttaat gtagctaaca ccccttttat acacatagag gaactactaa
    36361 atggatatgg cctagatatg ctaaacaaaa taatagaagg tacattcaat gccaatagta
    36421 ttacaaatat aaaagaaaca aatctatcat acaacagttt aataataaac tttatgaccc
    36481 aaaacttaga gcttttggac tctgttaaat tattcaacta tgtcaattct tccttattta
    36541 caataataga attaatagat gaaaacttaa agagcaaatt taagctagga tatatactac
    36601 attgctcttt tatgatagaa agatgtttta aaaatacaac ttttccatat aataacatta
    36661 ctgaattaat tagtaaaaat catgaactat atagtttaat acgtgaagcc tttaagccaa
    36721 cagaagctta ttttaatatt ttgataccag acgaagaaat tgcttatata atagacatga
    36781 ttaatgagta tctaaataaa taaaatttat agcacttatt ttgtgcatat taattaatat
    36841 atgaaatgga ggacgagatt ttgaaaggaa aaataaaaca gttaacaata cttgcattaa
    36901 tttttatatt tataactcct gtatttgcat ttgcagatac tccaccagta ccaaattctt
    36961 caagagcagc actacttata gaccaggaaa ctaaaagaat attatttgaa aaaaacattg
    37021 atgaaaaaat gccattagcg agtttaagta aaatgatgac ctttttattg gcaatagaag
    37081 cagttgataa aaatcaagtt aaagagactg atatggtgaa aatagacaag tctacagctt
    37141 cagtaggagg ttctacttgt aaacttaaag atggtgatga aatatctcta ggtgagctta
    37201 tgcagggtct aatgctagta tctggaaatg atgctgctat agccatagct aaacatattg
    37261 gtaaaactga aaagaatttt gtaaatatga tgaacaagaa agcagaagaa ataggaatga
    37321 ttgatacgta ttattttaat ccaaatgggc ttcctatata tactgaccca gaacataaag
    37381 aacctcctat tgaaaatatg tcaactgctc acgatatagt tacacttgga aagtatatgt
    37441 acgaccatta tgaaaatcaa gttacaagaa taaccactat gcaagtatat aatgatacta
    37501 aaaaggattt tacacattat aacacaaatc ctctgcttgt ttcagtacct ggtgttgatg
    37561 gaataaagac aggatataca gataatgctg gatattgcct tgcattttct atgatggtac
    37621 ctaaagatgc taaaaacgaa agaaatcata gattgatagg tgttgtactt ggtgatggta
    37681 acaagaaaaa tagaatttct tcttccgcta ctttattgaa atatggtaaa gataatttcc
    37741 attctaaaaa gatagcacat aagggtgata ttatagagac tccttgtgtt gatggtattg
    37801 atgattttaa gataacagtt aaggttgata aagatttgta tggtgttgtt tcagataacg
    37861 aaaatatcaa tcctaaggta gtgtttaaaa atatgaatta tcctatccat aagggagata
    37921 ttgttggtgt tgtaaagtat tataatgact ctggtaaatt tgttggaagt gttgatgtaa
    37981 aaagtgaaag taatatagga tgtattcctt tgaaggataa gattaagatt aaggttgcta
    38041 agataaataa aaaacttgag attaaaaatt ctgtttgttt taaagcatag taaagcatat
    38101 tattaaaagg gtatgttaaa tttataaaga ctccactttg tggagtcttt ttgtttttta
    38161 caaaaaatta aaatagtggc aacaattgag gatgtgaggg agagttgcca ctattttgta
    38221 tagggtatta tattatgttt ttaagctagt tttactatct tctatgttta tataataact
    38281 cttgtgtgtg aatttaattg ataattaata tgaaaattat gtgaagtttt aagttagttg
    38341 acttagacta ttgtaaattt tttcagaaaa attctgttca taagtctatt agaccaccga
    38401 attatttacc tataatattc cgatcaaaaa atctattctt ttttaaaagt aaggtcatga
    38461 ttattgtaca tatataaaaa ttccgaaaat taatctacac actagtaaca ttaagtataa
    38521 taaatttatt ttagtaaaac tattaaaata acctcataag aaactataga ttaaatcaat
    38581 caatctaatc tacagtttct tgattattcc ttaatattta taatttacat tatttgttta
    38641 ttttaaaact atctgttaca aatctagtca atgttgaaaa taaattcgct ctgaatatag
    38701 tagatgatg
//
LOCUS       DAEPIN010000032        38536 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.032, whole genome shotgun sequence.
ACCESSION   DAEPIN010000032 DAEPIN010000000
VERSION     DAEPIN010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 38536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38536
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.032"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            complement(307..846)
                     /gene="scpB"
                     /locus_tag="KR250_001863"
     CDS             complement(307..846)
                     /gene="scpB"
                     /locus_tag="KR250_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HBH0067799.1"
                     /translation="MKREDIKYIIESVMFAYGEPISIKELNYIINKELSSKEIEIMLN
                     LLIEEYREQNRGIQIIKLENKYQMCTNKDYAEYIKKIIEPKKKKSLSQATLETLTIIA
                     YKQPITKVEIEDIRGVKCDKVLQTLFENELIREAGRLNKIGKPIIYKTTDEFLKLLNI
                     ESLEELPPIENYQEVATNE"
     gene            complement(848..1567)
                     /locus_tag="KR250_001864"
     CDS             complement(848..1567)
                     /locus_tag="KR250_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HBH0067800.1"
                     /translation="MKYNIQLQVYEGPLDLLYDLITKHKIDIKDISIIDITKQYLNYL
                     KMLDKMDLEITSEFITMASKLLEIKSKYLLYKQKDEEEDPRIELMEKLEEYRKFKVAS
                     QDIKENITYVNERFYRNKEEIIIDDNVDLEDISIEAIKNILPYIFKVKTSQIENANDE
                     KLDKIVRKKIISVEEKILYIRDIIKEKIEVTFTNIIKSYENDEIIATFLSILELIKEK
                     EIVVVQDIFFDDILIRKSSEC"
     gene            complement(1597..2193)
                     /locus_tag="KR250_001865"
     CDS             complement(1597..2193)
                     /locus_tag="KR250_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HBH0067801.1"
                     /translation="MLSMNIGEIVASLVGIAMAISIHEFGHAYSAHLLGDDTAKAYGR
                     MTLNPVRHVDPIGLLAMFILKIGWAKPVPVNPNNFKNYKIGNLIVSLAGVFCNVLTAI
                     LCVIINKYVHMYAINTIAEYVFLYSIGFAAFNLLPIPPLDGWGVISTFVPYKWNEYLY
                     KFESMSYIILLIALFTGMYRIILTPIYAVLLKIVYLFV"
     gene            2491..3327
                     /locus_tag="KR250_001866"
     CDS             2491..3327
                     /locus_tag="KR250_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBH0067802.1"
                     /translation="MRQEERTVCFDRELKIEAYRFKGIMQKFPNHFHEHYVIGFIEKG
                     QRYLSCKNKEYTTSTGDLLLFNPFDSHTCEQIDDKVLDYRCINIKPEIMKKTVFEITG
                     KNYLPKFNQPVIFRSELVPLLQELHYIIMEEELDFKKEELFFFLIEQLIEEHTEPNLQ
                     SNLENTNIEIQAVCDYLENNYAEHIILDELSTIAGMNKYSLLRNFTKLKGITPYRYLE
                     NIRVNKAKKLLEKGVEPIDAAIQTGFVDQSHFTNFFKNFIGLTPKQYQNIFINDDSNN
                     FK"
     gene            3348..3770
                     /locus_tag="KR250_001867"
     CDS             3348..3770
                     /locus_tag="KR250_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2000 domain-containing protein"
                     /protein_id="HBH0067803.1"
                     /translation="MENKNSKCVMVIDENLPMGIISNTAAIMGITLGKHAPETVGPDV
                     IDKTGNSHLGIIDIPVPILKGNKEIIKDLRKKLYTLEFNDLTVVDFSDVAQSCNLYEE
                     FTQKIASVPEDELQYFGIAIYGNKKKVNKLTGSMPLLR"
     gene            complement(3981..5144)
                     /locus_tag="KR250_001868"
     CDS             complement(3981..5144)
                     /locus_tag="KR250_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBH0067804.1"
                     /translation="MKKLASTALAILIALTPLSFSFANNKENADANQLNISSKSAILM
                     DVGSGQILYEKDAHKKLPPASVTKVMTMLLIVEALDSGKIKLDDEVQVSETASSMGGS
                     QIFLEPGETQKVDTLLKGIAVASANDACVAMAEHLAGSVEGFVDRMNAKAKELNMNDT
                     HFANTNGLPVANHYTSAHDIALMSRELLKHEMISKYLTTWMDKVVVGKKQVTVGLANT
                     NKLIKHYQGATGVKTGFTQEAKYCLSASAKRGNTHLVAATLGAETSPERFNDASSLLT
                     YGFANYESVKLCSKGDNIATLTLDKADENKVKLVAKEDLNALIKKGSSKEFEKKIEIV
                     KNPKMPIKKGTVLGKIKICKDKKVIGEVELINTKDINKASYLQMLQRIIDNMI"
     gene            complement(5198..5398)
                     /locus_tag="KR250_001869"
     CDS             complement(5198..5398)
                     /locus_tag="KR250_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore protein"
                     /protein_id="HBH0067805.1"
                     /translation="MDDVSRQNAIKALKQTKMEIAGEYGMNYEDAFEIIENASNKGVL
                     EGYFKKLEKKKNLGQGISRHLE"
     gene            complement(5606..7282)
                     /locus_tag="KR250_001870"
     CDS             complement(5606..7282)
                     /locus_tag="KR250_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HBH0067806.1"
                     /translation="MQLFKKNTNKIKVMALGGLNEVGKNMTVVEYKDEIIVIDAGLSF
                     PEDEMLGVDIVIPDITYLVKNRDKIKGIFITHGHEDHIGALPYILKKINVPVYGARLS
                     IGLIQVKLKEHKMNNVKLNVIGPRQVIKLDNMEVEFLKNNHSIPDAYSIAIHTDQGII
                     YHTGDFKIDLTPIDGDVMDMHRICELSKKGVLLMLADSTNAEKPGFTMSEKTVGVGLD
                     ELFAKGNGRRIIVATFASNIHRLQQIINTAEKFNRKVAISGRSMVNVVGVAKELGYLD
                     ISDDMLIDLNDICKYEDSELVIITTGSQGEPMSALARMAFSEHKKVEIKSGDLVIISA
                     HPIPGNEKLISRVINFLFEKGAEVVYSDIADIHVSGHACQEELKLIHALVRPKFFMPA
                     HGEYRMLKRHAEIAEQLGMDKENIFVMQTGDVLELDKNSAKVANRIQTGNILVDGLGV
                     GDVGNIVLRDRKHLSEDGLMIVVVTISKDEGKVLAGPDIISRGFVYVRESEDLMDGAK
                     DIIKNVLNECEEKNIKEWAYLKNNIKENLKEYLYQKTKRNPMILPIIMEV"
     gene            7499..8236
                     /locus_tag="KR250_001871"
     CDS             7499..8236
                     /locus_tag="KR250_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBH0067807.1"
                     /translation="MRGKTHCAIGILTAIQTSLIFKIPISLVDILVSATFAVLPDLDK
                     SNSMVSNFILKNNVSKYIYRIFIYAVNIIIFFISININDNFYLSAIITFVAIIIIEAK
                     LTHTFLRKVFLSLIFILLAICLYIIEVEIYFTIFCLMLSIFPWLKHRSFSHSIFATII
                     VYFLLKQIELITNINNLSFYGTIGYASHMFLGDLFTKQGIPIFYPLSEKKISLGFLTV
                     GGPFSNFIEKSFIFVLIGLIIFSILKL"
     gene            complement(8353..8811)
                     /locus_tag="KR250_001872"
     CDS             complement(8353..8811)
                     /locus_tag="KR250_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBH0067808.1"
                     /translation="MANTMDLLKDKLKETGFKITPQRRAIVEILLKHDHSHLSSEEIY
                     DLVRVDCPEIGLATVYRTMQLLDEIGLISKLNLDDGCIRYEISLHKEDCHNHHHLICK
                     NCGKIMEAKEDLLDNIEKEIQSLYKFKILDHDVKFYGLCDECNGVSDSEE"
     gene            complement(9093..9380)
                     /locus_tag="KR250_001873"
     CDS             complement(9093..9380)
                     /locus_tag="KR250_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HBH0067809.1"
                     /translation="MEENIINLIDENGVESQFEIILTLEAEGKEYAILMPLDDEEAEE
                     ALIFRIDEDEEGEILIPLESDEEYETVVAVYTAIMEEEGLNYDEDESNGLN"
     gene            complement(9458..9883)
                     /gene="ruvX"
                     /locus_tag="KR250_001874"
     CDS             complement(9458..9883)
                     /gene="ruvX"
                     /locus_tag="KR250_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HBH0067810.1"
                     /translation="MLDGRIMGLDVGDKTIGVAVSDLMGLTAQGVKTIKRVGKKKDIE
                     ELKAIIKEKQVNKIVSGLPKNMNGTLGPQGEKVIKFCELVKAETGIDVEFWDERLSTV
                     AAERSLLEADVSRQKRKKVIDMLAAVIILQGYLDFKINS"
     gene            complement(10089..12260)
                     /locus_tag="KR250_001875"
     CDS             complement(10089..12260)
                     /locus_tag="KR250_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0067811.1"
                     /translation="MRKKIMSLFAIVIMIFVSTASYGFAEEKGKVIFIDMNRTNLGSM
                     MNIPILKNEIEKRGYVALMNIRGDQGTDDRRSCASMGAGTRASVTTEDYINFQKVDQD
                     TAKAFEVATGQKAKEINDLGINRSINENLEYGEYGANLGSLGKTLSENGLKTSVIGNA
                     DIIENNQLVKNRNLCLVAMDQYGRIDSGNIDNINKKDSKMPFGISTDYDKLLDETKKS
                     YKNSDAIFVELGDTYRLDMYRLNLNDNTYDTMKKNIVKNINKYLEQVFSMVGENDTVY
                     IASAFPSDLDYKNKRRLSPIIKFSGEGKGLLESATTRKAGIVANLDVGVDILGTFGLE
                     NKEMVGRSYTLVNKEDNISYLKSLYERIVSISSIRSTIINGFVGVISASWVIAMFAIF
                     FRNYMPHKERVFKVLKEFIKLGIIMPLSFMLAPLMNFKTPISISLGIFATTLILYLLG
                     RKLFKDNDIKHMGFFATLTILVIVIDSVFGTYLMQNNIMSYDAVIGARYYGVGNEYEG
                     VTIASAVFGLAVLLHYKKVSKLLVVIFSLIILITSAYPSMGANVGGAISECVAYLLFI
                     MLIYDIKIDFKKAVLLAVSAVVVVFAFAALDILSGSESHLGMFVQRIFLNGPGEILQT
                     FGRKIQMNMQLAQTSVWVNILLVGIGVIAVLIFKPSRHFRKIMNNFPILFKGFIASMV
                     GCIITLLVNDSGIVAASTASIYILIPLIIISINMIIFEKNN"
     gene            complement(12270..12527)
                     /locus_tag="KR250_001876"
     CDS             complement(12270..12527)
                     /locus_tag="KR250_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HBH0067812.1"
                     /translation="MEKDLDYTMKFEGIPEDRMSVGDTIDFVYKALVEKGYNPINQII
                     GYLLSGDSSYITSHKNARAIIKKFERDEILEEVITHYLNRK"
     gene            complement(12552..15191)
                     /gene="alaS"
                     /locus_tag="KR250_001877"
     CDS             complement(12552..15191)
                     /gene="alaS"
                     /locus_tag="KR250_001877"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HBH0067813.1"
                     /translation="MEKMGLNEIRSKFLEFFESKGHYVANSYSLVPNNDKSLLLINSG
                     MAPLKNYFSGVEVPPSVRMCTSQKCIRTGDIENVGITARHATFFEMMGNFSFGDYFKR
                     ESIKWGWEFVTEWLNIPEDKIWVTVYEEDDDSYDIWAKEMNFPEERMVRLGKDDNFWE
                     IGTGPCGPCSEIYFDRGEEYGCDNPDCKPGCDCDRYLEFWNHVFTQFDRDEEGNYSLL
                     ENKNIDTGMGLERMGCIMQGVDTIFEVDTIKSILEAVEKLTGVKYGENPKNDISIRII
                     TDHIRAVTFLVSDGVLPSNEGRGYVLRRLLRRAARHGKLLGVKELFLQKLIDEVIKVN
                     DKAYPVLVEKESYIKKVVGIEEEKFNETIDQGTEILNSYIEVLKNEGKTVLSGQEAFK
                     LYDTYGFPIDLTKEILEEEHLSVDEEAFNEEMEKQKERARNARGNMDGESWKEDPLSK
                     LESTVDSTFNGYSEIYGEGTIEAIVKDDELVQSAEEGDKVSIVLDNTTFYPEGGGQVG
                     DCGLITNENLVLEVLNTKKGANNSIKHIGIIKSGRISNGDKVKTLVDRETRMSAARNH
                     SATHLLHKALREVLGEHVNQAGSLVTPERLRFDITHFEAISNEELKVIEEKVNNVILS
                     SLDIKCDIMNIKEAKEKGATALFGEKYGDEVRVVSMGDYSTELCGGTHLTNTSQVGMF
                     KILSEGGVAAGVRRIEAITGKAVYEYLKERDGIISEVCVNLKSKEDNLIQRISSLLEE
                     NKNLSKELHDMKAKMSLQSVDSIFDSKVEVNGVNLITNKFEGMDMDTLRETADNLRDK
                     LGSGVVVLANVVDDKVNFVVTATKDVLDKGIHSGNIVREVAKIAGGKGGGRPNMAQAG
                     ASDVSKVDQALSYASEVIKTQVK"
     misc_binding    complement(15259..15497)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(15640..16719)
                     /gene="mnmA"
                     /locus_tag="KR250_001878"
     CDS             complement(15640..16719)
                     /gene="mnmA"
                     /locus_tag="KR250_001878"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HBH0067814.1"
                     /translation="MNKKVMIGMSGGVDSSVAAYLLKQQGYDVIGVTMKLWQDDDVVE
                     IEGGCCSLSAVEDARRVANKIGIPFYVLNFREVFKEKVIDYFIDEYLEGKTPNPCIAC
                     NKHIKFDDFYKKARQIGCDYVATGHYAKIEKDESTGRYLLKKSVTDKKDQTYALYNLT
                     QEQLEHTLLPIGDYEKDRVREIAKEMGMAVHNKPDSQEICFVKDNDYANYVKKHSKKR
                     IEEGFFVDTKGNILGKHKGILYYTIGQRKGLGITFGKPMFVIDINPINNTIVLGDNED
                     LFKKELIAKDVNFISIDTLEEPLRVQAKIRYSAKPSPATIHRVGEDTIKIVFDEAQRA
                     ITKGQSVVMYDGDIVVGGGIIEKSL"
     gene            complement(17008..17448)
                     /gene="nifU"
                     /locus_tag="KR250_001879"
     CDS             complement(17008..17448)
                     /gene="nifU"
                     /locus_tag="KR250_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold protein NifU"
                     /protein_id="HBH0067815.1"
                     /translation="MQYSDKVMEHFMNPRNMGEIDNASGVGEVGNPTCGDIMKIFLDI
                     DGDVIKDVKFKTFGCGSAIASSSMATEMIKGKTIKDALELTNKAVAEALDGLPPVKMH
                     CSVLAEQAVKAALIDYAQKNNIHIPELDGYVIDDAHDHDVEEEE"
     gene            complement(17502..18695)
                     /gene="nifS"
                     /locus_tag="KR250_001880"
     CDS             complement(17502..18695)
                     /gene="nifS"
                     /locus_tag="KR250_001880"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HBH0067816.1"
                     /translation="MEKRRLYMDYSATTPIKKEVLDAMMPYLTDYFGNASSFHTFGRE
                     AKDALDKAREQVAALINAEPSEIYFTAGGSESDNWTLEGVAYANKNKGNHIITSKIEH
                     HAILHTCEYLAKHHGFEITYLDVDSEGKVDLKQLEDSIKDTTILISIMFANNEIGTIQ
                     PIKEISEIAKKHKILFHTDAVQATGNIPVDVKELGIDLMSMSSHKIYGPKGVGALYIR
                     KGVRLHNFVHGGAQEKSKRAGTENIPAIVGYGKAAELAKENMQNHVETLTRLRNKLID
                     GVLERIPYTRVNGSLDNRLPGNANFAFQFIEGEGILLLLDMLGIAGSSGSACTSGSLD
                     PSHVLLAIGLPHEIAHGSLRLTVGDFTTDDDIDYILENLPKVIERLRSMSPLYDDAKK
                     QGLVK"
     gene            complement(18713..19159)
                     /locus_tag="KR250_001881"
     CDS             complement(18713..19159)
                     /locus_tag="KR250_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HBH0067817.1"
                     /translation="MKLSTKGRYGLKAMFELALNQDNGPVSLKFIAKKQKISDQYLEQ
                     IFSSLKKSGLVKSVRGAQGGYLLSKNAEDITVGDILVVLEGPVALSDCVLDEDVCENS
                     NMCVTKIVWEKMKKGIEDVIDSITLKDMINDYNKNKLENDITNIKK"
     gene            complement(19268..20038)
                     /locus_tag="KR250_001882"
     CDS             complement(19268..20038)
                     /locus_tag="KR250_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBH0067818.1"
                     /translation="MIIKLNSTYHSRVMKYLKKEPEYNLFIIGDIERYGYGNNFLNIW
                     ADVGEHGEIKAILLKYFEFMIFYSDGEYDVEGFYNLLRNTNYEEISGKICAVDALAKR
                     LGLNNLKVVDFCKLQTKKFLIDNNCNAKVKRIRLGNLKKTVKLYDLIDEFHSTTLENL
                     KNGLRTGRGYCIEINKQVVSMAKSTSENRTHAMIIGVGTHPKYRAKGLATKCLIKLCS
                     ELLRENKIPCLFYDNEEAGKIYKKLGFENIGKWGIYSK"
     gene            complement(20069..21334)
                     /locus_tag="KR250_001883"
     CDS             complement(20069..21334)
                     /locus_tag="KR250_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HBH0067819.1"
                     /translation="MPLADKIRPKTMNDVIGQSHIIGNGKILSKILQSNFLPNMIFFG
                     PPGVGKTTVAEIIAERSNKSFYKINATNSSLEDIKKVISELGSINNVNGVLLYIDEIQ
                     SFNKKQQQSILEFMENGSITLIASTTENPYHYVYKALLSRSTVFEFKPLEKLDVEKGL
                     KRAVEVLNEDSYMDIECNNNAIEDIAILSDGDMRRALNILEVVVYSTKPNKDNITYID
                     SDVIRASTFNKIINYDKNGDSHYDILSAFQKSIRGSDPQASIHYLARLIKGGDLISIC
                     RRLLVIASEDIGLAYPNAIVIVKACVDSAMQLGFPEAKIPLAEATILLATSPKSNSAC
                     IAIMKAMDELDKEFVKDIPNSIKDSHYSGSKEIGRGCGYKYPHNFKNHYVKQQYLPDS
                     IKDSIYYIPQENKTEKNIKKYLEYLNSDF"
     gene            complement(21441..22226)
                     /gene="codY"
                     /locus_tag="KR250_001884"
     CDS             complement(21441..22226)
                     /gene="codY"
                     /locus_tag="KR250_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HBH0067820.1"
                     /translation="MASEVLQKTRKINKTLQTSGGSSVSFDLLAGALGDVLSSNVYVV
                     SAKGKVLGLHLNDVQDSSVIEDEYTKQKKFSDEYTQNVLKIDETLENLNGEKILEIFP
                     EEHGRLQKYTTVVPILGSGQRLGTLVLSRYSNSFNDDDLVIAEYSATVVGLEILRAIG
                     EELEEEMRKKAVVQMAIGTLSYSELEAVEHIFAELDGKEGLLVASKIADRVGITRSVI
                     VNALRKFESAGVIESRSLGMKGTHIRILNDKLTDELKKLKNNQ"
     gene            complement(22391..24478)
                     /gene="topA"
                     /locus_tag="KR250_001885"
     CDS             complement(22391..24478)
                     /gene="topA"
                     /locus_tag="KR250_001885"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HBH0067821.1"
                     /translation="MAKTLVIVESPAKAKTIEKFLGKSHYTVKASVGHVRDLPKSKLG
                     VDIENNFEPQYINIRGKGDVIKELKKEAKKSKQVYLATDPDREGEAISWHLAHILNLD
                     ESEDCRIEFNEITKDAIKKAIKHPRNIDINLVDAQQARRVLDRLLGYQISPILWQKVR
                     KGLSAGRVQSVTTKLICDREKEIKAFIPKEYWTIDVEAKTVKDKDITLKFYGKNDEKI
                     ELENEEAVNEILKEMEGKDLKVEKIESKSRRKSAPKPFTTSMLQQEGANKLFFTTKKT
                     MMIAQELYEGIDIEGEGTVGLISYIRTDSKRISDEAKEKAKGYIVNNLGENYYKNTGD
                     KKEKKDTKKVQDAHEAIRPTSVDRTPESIKASLSKDQYKLYNLIWRRFVASQMEDSVF
                     DILNVECKIGDIVFKATGSKMKFDGYTKVYNFSEREDKILPSIEKGDILNIENILPDQ
                     HFTQPPARYTEASLVKTLEELGIGRPSTYAPTIATILNREYVEKQGTSLCPTELGIIV
                     TDILENNFHKFIDVDFTADMESKLDVIEEGNTDWKKIVSESYAPLKEAIEEALENIEK
                     VNMDEETDEICENCGSNMVIKYGRFGKFMACKNYPDCKTTRPIMNKIGVKCPKCEEGD
                     IILRKSKRGKAFYGCSNYPNCDFVAWNKPTGEFCEKCGSYMVEKVTKSETKTVCSNKE
                     CNKETKEHNDGKE"
     gene            complement(24511..25611)
                     /gene="dprA"
                     /locus_tag="KR250_001886"
     CDS             complement(24511..25611)
                     /gene="dprA"
                     /locus_tag="KR250_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HBH0067822.1"
                     /translation="MEKRDTYLWLKSIGGITTKTIEIIENEIVNIEDIFDFSEKEIYN
                     LKNISLNIRKNIVKYRGHAYLENIKELLYKKAIKYICKYDKEYPENLKNIYNAPKLLF
                     YKGDIGLVNNNFNIAIVGSRKPTAYGINCAKTISCQLSQYGVNIVSGLAIGIDAYSHI
                     GCMSGKSKTIAVLGSGVDNPLPKQNLHLSNKILENGGLLLSEYNINSTVAPYHFSNRN
                     RIISGLSDGVVVVEAAIKSGALITVDFALEHGKNVFAIPGNINSQMSRGCHKIIKEGA
                     KLIENIDDILNEYNIFNIIDKKINQKYDNISLNAKSKQIIEAIKREGNLHIDSICDYT
                     GIEIKYVNSIINELVLNELVVEMNNKTYSLNV"
     gene            complement(25630..27231)
                     /locus_tag="KR250_001887"
     CDS             complement(25630..27231)
                     /locus_tag="KR250_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HBH0067823.1"
                     /translation="MLSIINSSNLVGIDSFLVKVEVDVSNGIPSFNIVGLPGKEIKEA
                     RERVKSAILNSGYKFPSTRIVVNLSPADIKKEGAFLDLSISIGLLRELIKKDENYIRE
                     SMFIGELSLDGKIRKVRGILPIIMGAKTQNIKRIFIPIENIKESLFVDEIDIIPIKSL
                     KECVDFLNEEIKVDKVRIMSFLDDKSRKENEELEKDNSYIDCKYTKINNEESKYDEDF
                     KDVKGNYFVKRSAEIAAAGNHNMFMIGPPGSGKTMIAKRVRTILPDISVEEMIEVSKV
                     YSILGMINETKGIIDKRPFRAPHHTTTKQSLIGGGMDARPGEIALAHRGILFLDEIAE
                     FDRKILETLRQPIEDGYVNISRVKYSAKYPCRVLLVAAMNPCPCGYYMSETECRCRSN
                     EIDRYINKISGPLLDRFDIFVEVNSIKYSDFNSLKQEESSQKIKRRVENARKIQINRF
                     KKDNIKNNSEIKAYNLFKYCKLEKEASKTAEMIFNKYNLSSRSYTKLLKMARTIADLE
                     ERDLINSQCIIEAFSFRKAYYSYFK"
     gene            complement(27262..27606)
                     /locus_tag="KR250_001888"
     CDS             complement(27262..27606)
                     /locus_tag="KR250_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HBH0067824.1"
                     /translation="MNNKEKGDFGEKVAVNYLLSKGAKILEKNYRLKIGEIDIIAKME
                     DEIIFVEVKSRSNIKFGYPCESVSFKKRKKIIGVANYYIIKNNLNNTPVRFDVIEVYL
                     LEKRINHIMNAF"
     gene            27790..28953
                     /locus_tag="KR250_001889"
     CDS             27790..28953
                     /locus_tag="KR250_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBH0067825.1"
                     /translation="MNREKLVAFIRYLSLFILVVSYLKNVENTLYPVITMIILFLIII
                     NNQVRFFSLSNENKFILISYFLELILIFILCNYTKTFNSIYFVPLILDISFLIKEKYK
                     YILFSFVIISSLIISLDKNIYLALESSSILLIITVLSIYIENENLSKLYSQNLYDKLR
                     ISKDELKKVNADLETYASSIEELAILKERNRISREIHDSVGHSLSTTIIQLNAIEKLL
                     KDKPLIYDLVHELREFVTESFQDVRRAISELKPVEYENYQSLFKIKELVKSFIKLTNI
                     NVKLTISKNTWNLSRNQSITLYRLIQESLSNSSRHGKATEIRIFITFNTSNLIITISD
                     NGIGCGNIKKGNGLNSINERIYELNGKVEFDNSNNGFTIRASFPKFSGGDFFE"
     gene            28946..29593
                     /locus_tag="KR250_001890"
     CDS             28946..29593
                     /locus_tag="KR250_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0067826.1"
                     /translation="MNKIKVLIVDDEKLIRKGLKIILSSYNDLEIVGDASNGYEALEF
                     CKMNDVDIVLMDIRMKVCDGVLGTRLIKEYNNSITLLILTTFNDDEYIKDAMKFGASG
                     YLLKDSSDEVLHEGIRSSFFGNIVLDKSVAEKIMTSEKTVKQEHLYDMYNLTEKEISI
                     IRLIANGLNNKEISQELFLSEGTIKNNITNILAKLELRDRTQLAIFAFKNKIVIE"
     gene            29722..30657
                     /locus_tag="KR250_001891"
     CDS             29722..30657
                     /locus_tag="KR250_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0067827.1"
                     /translation="MDIVRVNNITKRFNDKLVLDNISFSVKKGEIFGLIGPNGAGKST
                     LINIITNLMLPNSGSIQINGLDLSKDYIKAKSIIGLVPQELAIIETLTPFDNLEYFGA
                     FYGLKGKLLKERIIEALEVTGLTEVKKKKVKKLSGGMQRRLNIGIALLNHPKILILDE
                     PTVGVDPQSRNHIFNFIKDISKKHETTVIYTSHYMEEVEHLCSKIFIMDEGKEIAFGD
                     NDYLKSLVSTNTKLIIEIKNINAQLIFDLKNTKGVISVLENNSLLELDIDKKLQLTDI
                     LSVIDKNNSKIMKISYEEPSLEDVFLNLTGKNLRD"
     gene            30671..31768
                     /locus_tag="KR250_001892"
     CDS             30671..31768
                     /locus_tag="KR250_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0067828.1"
                     /translation="MKLYMSVKTMLKSLKSSFILNLIYFLALPLILSWFLGMVTESMF
                     QNPIKTESTPIVIYDKDNTRLSNDLTKYLKNDLSYILTVKKDDSKAELKLTIPKGYES
                     SLLNEKSNTLNIEKLGTRDDIAILLQDILDTYHEKFYLNNSQKISSEDFSKLFNKNSI
                     DTSIINNNVKQNSYEYFALVSLGFLVIIFIMNNILSNYISESKGLSKRLYSMPITRVQ
                     FLIYDFVGLWIYSFIFLLLYVLFFRILGITFKGNFAILLLLCALSSYFMTSISTFVTS
                     FFSKKYGTAIVYALLFLQTIFGGIFSMISDTFTKLTSLSPTYLIGELFSNYETFKTID
                     SIGDLIFTCLIISTILIALAIVKEKYKWREV"
     gene            31771..32835
                     /locus_tag="KR250_001893"
     CDS             31771..32835
                     /locus_tag="KR250_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0067829.1"
                     /translation="MIRLTIANIKRYLKNHNLLINMVMLPIILIFSLNFFINNSGNQS
                     MYFSPVAIVSDSSGKYEHKLINSSKLKENSFRLNELDKAMNLLKNNKVSAVFVLDKNF
                     SNSIDKLQRPVVKCFKIENGGGSLWAESQIESFITKSLKLKVDKNIDDKLTKTNIIDN
                     KPDKNKGSFLVVFLICYFMYINAAHLASDLFTLKKSNVLKRLISTDNKDIKIIFSIFL
                     GLFFIQSIVYTFALLCFSIIEDFNLSLNVLMIVLANSFVSTGFVFWVARVFKNESSIS
                     LISTFYSLIGLAISISSLIPSMDKLSFMTNLSKFTPFYWTIDAIKNNGSILINIIALI
                     LIGIIFVTAGSLKLRDFAKN"
     gene            33079..33576
                     /locus_tag="KR250_001894"
     CDS             33079..33576
                     /locus_tag="KR250_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HBH0067830.1"
                     /translation="MYKIGESVMYPKEGACSVNDIVTKKINHEMQKYYELSVIFNSNL
                     KISIPVLNADRIGIRPVMDGNDVDNFIQSINKTDGVWIFDRKERLKLYQDKFHSGDVF
                     EIVKLIKMLMIQDSSKQLCSTDKEFLNKAQKFALSELAAAQCKSYTMVLEEMKKHILN
                     SKNTN"
     gene            complement(33752..34000)
                     /locus_tag="KR250_001895"
     CDS             complement(33752..34000)
                     /locus_tag="KR250_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0067831.1"
                     /translation="MSRKAYEEALVELEKFIDERKEIIKSAEDCIDKYIVDRTLPFDY
                     KDKCVEWQQELLDIAEAQVLEANELSVLLQEKKELEED"
     gene            complement(34066..35082)
                     /locus_tag="KR250_001896"
     CDS             complement(34066..35082)
                     /locus_tag="KR250_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBH0067832.1"
                     /translation="MEYNELLKSARENLNGSCKVCKVCNGVACAGEVPGMGGKGSGSS
                     FIENSKSLEKVKVNMRVIHDVSNPDTSIEMFGKKMSAPIFAAPVTGTTLNMGGKINER
                     DYIEPVVAGCANSGIYAMVGDTAVDAFLMENLDVVKKYNGAGIVFIKPWDNENIIKKI
                     RLAEEAGAFAVGVDIDACGLVTLSLHGKPVLPKNVKQIKELVKSTKLPFILKGIMTVE
                     DALMAVEAGVDAIVVSNHGGRVLDCTPGACEVLPKIADAVKGKVTILADGGVRTGLDV
                     LKMIGLGADAVLIGRPFVTASFGGATDGVETYVNKLQSELSSSMILTGCQTIKDIDGK
                     VIYK"
     gene            complement(35140..35907)
                     /locus_tag="KR250_001897"
     CDS             complement(35140..35907)
                     /locus_tag="KR250_001897"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HBH0067833.1"
                     /translation="MQDKSVREIKEIIETLEVEKYMEYIELLRVDERKSVQGLAIKLA
                     KKLDNIRKEEERLETINIFENEGYDKGYLYIGGIDEAGRGPLAGPVVASVVVFKKDTK
                     IEGVNDSKKLSEAKRDELFEVIKEEALDYGIGIVNNEEIDEFNILNATYMAMKKAINC
                     LKKAPDYLLVDAATIPGIDISQNPIVKGDSKSISIAAASILAKVTRDSIMYQYDRVYP
                     EYGFKSHKGYGTKEHYEAIEKYGITPIHRKSFLKNIL"
     gene            complement(35975..38164)
                     /locus_tag="KR250_001898"
     CDS             complement(35975..38164)
                     /locus_tag="KR250_001898"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HBH0067834.1"
                     /translation="MKYIIKRDGTKELFSKSKIEDAVYKASINSEGGVDRELAYDISR
                     KIANDYNSMEKCLSVEEIQDIVEVLLMESNRKDIAKHYILYRERRSEIRTKAWEFDEL
                     QKSIWKNKYRHNGESFDEWIKRISNNSSRISKLIRQKRFLFAGRILANRGLQKEGKKV
                     TYSNCYVIEPPKDSIEDIFDTAKKLARTFSYGGGVGIDISKLRPKGAKVNNAAKHTTG
                     AVSFMELYSMTTGLIGQRGRRGALMISMDVNHPEIEDFIDIKTDLEKVTKANISVRVT
                     DEFMQAVREKKLYNCTFEVETTGEKIVKIVNAYDLFNKFVKNNWDYAEPGILFWDKIK
                     TYNFMSEDKNFCFSGVNPCAEEPLPSGGSCLLGSINLSEFIIQPFGEHATFDINKLKS
                     CVREAVVALNDVLDEGLNLHPLQEQRDSVHKYRQIGLGVMGIADMLIKMGIRYGSDES
                     LELCDLIGKTMLNEAVKQSALIAKEYGTFSEYKYENISKSKFFIENLNEDTKEMVKKY
                     GLRNSQLLTIPPTGSISTMIGISGGIEPIFNLSYIRKTESLHDEDVYYKVYTPIVKEY
                     MDKKGITDEECLPDFFVTAMNIGYEERINMQKVWQKYIDASISSTINIPFETTVEDVF
                     NIYIKAWEAGLKGVTIFRDGCKRSGILINEKKEENNKEDKKENSKKEYYKNNKKEKDN
                     KDEKAKEIINKNFEEDEKFVCPECGSEGIAHTGGCSICLKCGYSGCN"
BASE COUNT        12384 a         6621 c         4116 g        15415 t
ORIGIN      
        1 taagctttaa acaaatatct ttgtttttta tatcagctat tattaagatg tttatatgag
       61 atattaaaat caacatagtt acacttataa taaaaattac agctattatt aaatacttaa
      121 aattaaataa ttcattaagc tccatgttta tattatcatc aaaaaattac tatttatact
      181 taattatcca tatttatttt aagagaatta taattttata tttatacaaa aaaacacaac
      241 aacttagagc attttaaatc ttttaaaatt taaaattaca tactaaatta ttgtgatttt
      301 atatttttat tcatttgttg ccacttcttg ataattttct ataggtggca attcttcaag
      361 actttctata ttcaacaact ttaaaaattc atctgttgtt ttataaatta ttggtttccc
      421 tatcttattc aatctaccag cttctcttat aagttcattt tcaaacaaag tttgtaatac
      481 cttatcacat tttactcctc ttatatcctc tatttctacc tttgttattg gttgtttgta
      541 ggcaattata gtaagagttt ctaatgtagc ttggctcaaa cttttctttt tctttggttc
      601 aataatcttc tttatatatt ctgcatagtc tttatttgta cacatttggt acttattctc
      661 taacttgatt atttgaatac ctctattttg ttctctgtac tcctcaataa gtaaatttaa
      721 cataatctct atttcttttg atgaaagttc tttatttatt atatagttta attcttttat
      781 acttattggc tcaccatatg caaacataac agattctata atatacttta tatcttctct
      841 tttcatttta acactccgaa ctttttctta ttagaatatc atcaaaaaat atatcttgta
      901 ctacgactat ttctttttct tttataagct ctaatataga taaaaaagta gctattattt
      961 catcattttc atatgatttt ataatatttg taaaagtaac ttcaatcttc tcttttataa
     1021 tatctcttat atataatatt ttttcttcta cagatattat tttcttcctt actattttat
     1081 ctagtttctc atcattagca ttttctatct gtgatgtttt aactttaaat atgtatggaa
     1141 gtatattttt tattgcttca attgatatat cttctaaatc cacattgtca tcaataatta
     1201 tctcttcttt atttctataa aatctctcat taacataagt tatattttct tttatatctt
     1261 gtgatgctac cttaaacttt ctatattctt ctaacttttc cataagttct attcttgggt
     1321 cttcttcttc atctttttgc ttataaagca gatatttaga ctttatctct aaaagttttg
     1381 atgccatagt tataaattca ctagttatct ctaaatccat tttatctagc attttcaaat
     1441 aatttagata ctgcttagtt atatctatta tggatatatc ctttatatct attttatgct
     1501 ttgttattaa atcataaagt aaatctaatg gtccctcata gacttgtaat tgtatattat
     1561 acttcatatc aatccctctt taatctgttt attatattaa acaaataaat atactatttt
     1621 tagcaacact gcatatattg gtgttaatat tattctatac ataccagtaa ataaagcaat
     1681 taataaaatt atataactca tactttcaaa tttatacaaa tattcattcc atttatatgg
     1741 tacaaatgta gatataactc cccatccatc aagtggaggt atcggaagta agttaaaagc
     1801 tgcaaatcca atactatata aaaacacata ttcagctatt gtatttatag catacatatg
     1861 cacatactta ttgataatta cacacagtat agcagtcaaa acattacaaa aaacgcctgc
     1921 aagtgaaact atcaggtttc ctatcttata gtttttaaag ttatttggat ttactggtac
     1981 tggctttgcc caccctatct taaggataaa catagccaaa agacctattg ggtcaacatg
     2041 tcttacagga tttaatgtca ttctaccata agctttagct gtatcatctc ccaataagtg
     2101 agctgaataa gcatggccaa actcatgtat tgatattgcc atagctatac caactaaact
     2161 tgctactatt tctcctatat tcatacttaa cattaatgta catctcctaa attaaatttt
     2221 taatattatt ataacaatta aatagcttat aatcaaaatt attttacatt ataataattt
     2281 taacttctag gcataaaatt aaaatttgct ttttatttta taacttttat tattatatca
     2341 tattcattgt gaaaatataa taattacacc tactgtaagt gtaatattat attgacatga
     2401 attgacatta ttgtctctta gtcaatatta tacaagaagt ataaaaaaat atgtgttaca
     2461 ctttgtataa catgaaagga ggttacctaa gtgagacaag aagaacgcac agtatgcttt
     2521 gatagggaat taaaaattga agcatataga tttaaaggca taatgcaaaa attcccaaac
     2581 cattttcatg aacattatgt aattggtttt atagaaaaag gtcaaagata tttatcttgt
     2641 aaaaataaag agtataccac aagtactgga gatttgctac tttttaaccc ttttgatagt
     2701 catacttgtg aacaaataga tgataaagta cttgattaca gatgtattaa tattaagcca
     2761 gaaataatga aaaaaaccgt ctttgaaatt acaggtaaaa attatttacc aaaatttaat
     2821 caacctgtta tatttcgcag tgagttagta cctctactac aagaattaca ctatataatc
     2881 atggaagaag aattggattt taaaaaggaa gagctttttt tcttcctaat tgaacaatta
     2941 attgaagaac atacagagcc aaatttgcaa tctaacttgg aaaatacaaa tatagaaatt
     3001 caagcagtat gtgattatct agaaaataat tatgctgaac atataatact tgacgaatta
     3061 agcactatag caggtatgaa taagtactct ctacttcgta attttacgaa attaaaaggt
     3121 attacaccat atagatacct agaaaatata cgggtaaaca aagctaaaaa gcttttagaa
     3181 aaaggcgtag aacctataga tgctgcgata caaacaggat ttgtagacca aagtcacttt
     3241 acaaactttt ttaagaattt tattggtctt acaccaaagc agtatcaaaa tatatttata
     3301 aatgatgatt caaacaactt taaataaggt aaaaaggaga aataaaaatg gaaaataaaa
     3361 attcaaaatg tgtcatggta atagacgaaa atttaccaat gggaattata tccaatacag
     3421 cagctataat gggaattaca ctaggaaaac atgccccaga aactgttggg ccagatgtaa
     3481 ttgacaaaac aggtaatagc cacttaggaa ttattgacat tcctgtacct attttaaaag
     3541 gaaacaaaga aataataaaa gatttacgta aaaaattata tactttggaa tttaacgatt
     3601 taactgttgt agatttttca gatgtagcac aaagttgcaa tctttatgaa gaatttacac
     3661 aaaagattgc tagtgttcca gaagatgagt tacagtattt tgggatagca atttatggga
     3721 ataaaaagaa agtgaataaa cttactggta gtatgccttt acttagataa ttgttaaaca
     3781 accctatata tgatattaaa tcaaatgata ttttaagata cataaacttt tctatatttc
     3841 tataaaaatc ataagattat aaaatacttc tatatagtaa ataaaaatac ccctatggca
     3901 aataattaca taagggtatt ttttatgtgt attataatca tatctttgtg tattataatt
     3961 atatttcatc tgataattat ttatatcata ttatcaatta ttctttgaag catttgtaga
     4021 tagcttgcct tatttatatc tttagtattt attaattcca cttcgcctat tactttttta
     4081 tctttacata tctttatctt tcctaaaaca gttccttttt taattggcat cttaggattt
     4141 tttactattt caattttctt ctcaaattct ttagaacttc cttttttaat aagagcattt
     4201 aaatcttctt tagcaaccaa tttaactttg ttttcatcag ccttatccaa tgtaagtgtt
     4261 gctatattat cacctttact acataactta acactttcat aattagcaaa tccataggtt
     4321 aaaaggctag aggcatcatt aaatctttct ggagaagtct ctgctcctaa agttgcagca
     4381 actagatgag tattacctct ctttgcagat gctgataaac agtatttcgc ttcttgagta
     4441 aagccagttt taacaccagt tgctccttga taatgcttta taagtttgtt agtatttgca
     4501 agacctactg taacttgttt cttccctact acaaccttat ccatccatgt agttaagtat
     4561 ttacttatca tttcgtgttt taatagttct ctagacatta aagctatatc atgagcagat
     4621 gtatagtggt ttgcaactgg aagtccattt gtatttgcaa aatgagtatc attcatattt
     4681 aactcttttg ctttagcatt cattctatca acaaaccctt caacacttcc agctaagtgt
     4741 tcagccatag caacacaagc atcatttgca gaagcaacag ctataccttt taacaatgta
     4801 tcaacctttt gagtttctcc tggctctagg aatatttgac taccacccat actagatgca
     4861 gtttcactta cttgaacttc atcatcaagt ttaatcttgc ctgaatctaa agcttctact
     4921 ataagtagca tagtcattac cttagttaca ctagcaggag gaagcttttt atgagcatcc
     4981 ttttcatata aaatttgtcc actaccaaca tccatcaaaa ttgctgattt tgaagatata
     5041 tttagttggt tggcatcagc attttcctta ttgtttgcaa aagaaaagct taagggagta
     5101 agcgcaatta atatagccaa agcagtacta gctaattttt tcatcgatgt cacctcttaa
     5161 agtttattta aacttatttt ctcacattta acttttttta ttctaaatgc ctagatatac
     5221 cttgacctaa attttttttc ttttcaagct ttttaaagta accttctaaa acacctttat
     5281 tagacgcatt ttctattatt tcaaaagcat cttcataatt cattccatac tctcctgcta
     5341 tttccatttt tgtttgtttt aaagctttta tagcattttg tcttgaaaca tcatccataa
     5401 gaatcctcct atcagtaaaa tttatatata agtagttttt actaataaaa gattgttata
     5461 attggtattt tatgtaatat atatattttt ttaaatcaag tattaaaaag acctcttgta
     5521 attcttaaga taatatcaat tggatttttg atattacttt aaaaattaca agaggtctaa
     5581 ttttcaaatt tatagttttt ttatattata cttccattat gataggaagt atcattggat
     5641 ttcgtttagt tttttgatat aaatattctt ttaggttttc tttaatatta ttctttaaat
     5701 aagcccattc tttaatattt ttctcttcac attcatttag aacattctta attatatcct
     5761 ttgctccatc cattaaatct tctgattctc ttacatatac gaatcctctg gaaattatat
     5821 ctggacctgc taaaacttta ccctcatcct tagaaatagt aactacaact atcataagac
     5881 catcttctga taaatgcttt ctatctctta aaactatatt acctacatcg cctactccta
     5941 gaccatcaac taatatgttt cctgtttgta tacgatttgc taccttagca gagtttttat
     6001 ctaattctaa aacatctcct gtttgcatga caaaaatatt ttctttatcc ataccaagct
     6061 gttcagctat ttcagcatgt ctttttaaca ttctatattc accatgagct ggcataaaaa
     6121 actttggtct aactaatgca tgtataagtt ttaattcttc ttgacaggca tgtccagaaa
     6181 cgtgtatatc agctatatca ctgtatacta cttctgctcc tttttcaaat aagaaattga
     6241 ttactcttga tattaatttt tcatttcctg gtattggatg agcagatatt ataactaaat
     6301 cgccactctt tatttctact tttttgtgct ctgaaaaagc cattctagca agagctgaca
     6361 taggttcacc ttgtgaacca gttgttatta tcactaattc actatcttca tacttacata
     6421 tatcatttaa atctataagc atgtcatcag aaatatctag atatcctaat tcttttgcaa
     6481 ctccaactac attgaccatt gagcgacctg atatagcaac ttttctatta aacttctcag
     6541 cagtatttat tatttgttgt aatctgtgta tgttggatgc aaaggtagca actataattc
     6601 ttctgccatt tccttttgca aataattcgt caagacctac cccaacagtc ttttcagaca
     6661 tagtaaatcc tggtttttct gcattagtac tatcagccag cattaaaaga actccttttt
     6721 tgctcaactc acagattcta tgcatatcca taacatctcc atcaatagga gtcaagtcaa
     6781 tcttaaaatc tcctgtgtgg tatattatac cttggtctgt gtgtattgca atagagtaag
     6841 catctggtat actatggtta tttttcaaaa actctacttc catattatca agctttataa
     6901 cttgtctagg acctataaca tttaacttaa cattattcat cttatgttct ttaagtttta
     6961 cttgtataag acctatacta agtcttgccc catatacagg tacatttatt ttctttaata
     7021 tatatggaag agctcctata tggtcttcat gtccatgtgt aataaatata ccctttattt
     7081 tatccctatt ttttactaaa taagttatat ctggtataac tatatctact cctaacattt
     7141 catcctctgg aaaactaagt ccagcatcta taacaattat ttcatcttta tactcaacaa
     7201 cagtcatatt ttttccaact tcgttaagtc cacccaaggc cattaccttt atcttattgg
     7261 tgttcttttt gaacaattgc atcatctcct tctgtctatt taatttttta acacgttcta
     7321 caatcataaa taatatcaaa tcacgcactc gctaccagtt attcttattg taccataatt
     7381 cgtatcatat tttaagctat atttaaaata catttaacgt attattttga caaacctatt
     7441 ttattttgat atcctctaaa tattatatga tttaatatcg gataatttag gaggatttat
     7501 gcgtggtaaa actcattgtg caataggaat cttaacagct atccagacat ctttaatatt
     7561 taaaattcct atatctttag ttgatatttt agtttcagct acttttgctg tactacccga
     7621 tttggataaa tctaattcta tggtctcaaa tttcattcta aaaaacaatg tatccaaata
     7681 catatataga atttttatat atgctgttaa tattattatt ttctttattt caataaatat
     7741 aaatgataat ttttatttaa gcgcaataat tacatttgtt gcaattataa ttattgaagc
     7801 aaaactaacc catacatttc taagaaaagt ttttctatca ttaatattta tactattagc
     7861 tatatgctta tacatcatag aagttgaaat atattttaca attttttgtc taatgttgtc
     7921 aattttcccc tggttaaagc acagaagttt ttctcatagc atatttgcaa ctataatagt
     7981 atatttttta ctaaaacaga tagaattaat cactaatatt aataatttat ctttttatgg
     8041 tacgattggt tatgcaagcc atatgttttt aggagattta tttacaaagc aaggaatacc
     8101 aattttttat cccctaagcg aaaagaaaat ttctttaggg tttttaactg ttggaggacc
     8161 atttagtaat ttcatagaaa aatcattcat ttttgtactt ataggattaa ttatattttc
     8221 aatattaaaa ctataatcac tttgaaaact gtatatttgc attttataga tggtttataa
     8281 taaaaaatgg aagaatagca tagaatataa aagttctata ctattcttcc atcgacatta
     8341 agcaatagaa gtttattctt ctgaatcgct tactccatta cactcgtcac atagtccata
     8401 aaatttaaca tcatgatcaa gtattttaaa tttgtacaat gattgtatct ctttttctat
     8461 attgtctaaa aggtcttctt tagcttccat tatctttcca caatttttac aaataagatg
     8521 atgatgatta tgacagtctt ctttatgtag actaatctca tatcttatac atccatcgtc
     8581 taagtttagt tttgatatta atcctatttc atccaatagc tgcattgttc tatatacagt
     8641 agctaagcct atttcaggac agtcaaccct aactaaatca tagatttcct cactacttag
     8701 atgtgaatgg tcatgtttaa gcaatatttc aactattgct cttctttgtg gcgtaatctt
     8761 aaaaccagtt tcttttaact tgtcttttaa taaatccatt gtatttgcca taacatcacc
     8821 cttactattt tcaattgata atctttatta aataatattg taattgataa ttcatttttt
     8881 gtcaagacat ttaaataaat ttaatttatt ctcataatat aaatatatac ccataaaaga
     8941 tatattattc accaaaattc ataaattttt tggtattttt aataaaaaac aaaaaaacag
     9001 atatatacta taaatgcttt tacaattaat aaggtttaat taaagcaaat ataataaata
     9061 tctgattttt atatacaaac ttaattactt atctagttaa gtccatttga ttcatcttcg
     9121 tcgtaattta aaccttcttc ttccattata gcagtataca cagctacaac agtttcatac
     9181 tcttcatcac tttccaaagg tattaagatt tcaccttctt catcctcatc aattctaaag
     9241 attaaagctt cttcagcttc ttcatcatct aaaggcatta atattgcata ctcttttcct
     9301 tctgcttcta atgttaatat tatttcaaat tggctttcaa cgccattttc gtctattaaa
     9361 tttattatat tttcttccat aatgtcattc acctcaaatt tatttttatt tttattattt
     9421 atattatata tcataagtat tcaattttat agttctttta tgaatttatt ttaaaatcta
     9481 aatatccttg caaaattatt actgctgcca acatatctat aacctttttt cttttttgtc
     9541 tactaacatc tgcttctaat aaagacctct ctgccgctac tgtagaaagc ctctcatccc
     9601 aaaactctac atcaatacct gtttctgctt ttaccaattc acaaaacttg attacttttt
     9661 ctccttgtgg acctaaagtt ccattcatat tttttggtaa tcctgataca attttattta
     9721 cttgtttttc ttttattatt gcttttagct cttcaatatc ttttttcttg ccaactcttt
     9781 ttattgtttt tacaccttgt gcagttaatc ccatcaaatc actcacagca actccaatag
     9841 ttttatcacc aacatcaaga cccattattc ttccatctaa catagttcct cctttatcta
     9901 tttatattct atagattaaa tacatattct gaacctctca taactaaggt cacaggctta
     9961 ctgcttcata gaattttaca ttatcaatca taaaaattgc aatatgattt tttatgtaag
    10021 catccagttt acattaataa tcatattgca ttttttattt aaatcctata tgaacagtaa
    10081 aaattttttt agttattttt ttcaaatatt atcatattta tacttattat tattagtggt
    10141 ataagtatat atatagatgc tgtagatgca gcaactattc cagaatcatt aactaaaaga
    10201 gtaattatac aaccaaccat agatgcaata aaccctttaa acaagattgg aaaattattc
    10261 attatctttc taaagtgtct agatggttta aatataagca ctgcaattac tccaattcct
    10321 acaagtaaaa tatttaccca tacacttgtc tgtgctagtt gcatattcat ttgaattttt
    10381 cttccaaatg tttgtaatat ctctcctggt ccatttagga aaattctctg tacaaacata
    10441 cctaaatgag attcactacc tgaaagtata tctaaagctg caaatgcaaa aacgacaact
    10501 acagctgaaa cagctaataa tacagctttc ttaaaatcta ttttaatgtc atatataagc
    10561 attataaaca acaaatatgc tacacattct gatatagctc caccaacatt tgcacccatt
    10621 gaaggatatg cactagttat aagtattatt aatgaaaaaa ttacaacaag taatttagat
    10681 accttcttat aatgtaataa tactgctaag ccaaatactg cacttgctat agttacacct
    10741 tcatattcat ttcctacacc atagtaccta gctcctatga cagcatcata actcattatg
    10801 ttattttgca ttaagtatgt tccaaataca gaatctataa ctatgactaa aatagtaagt
    10861 gttgcaaaaa atcccatgtg ctttatatca ttgtccttaa ataattttct acctaacaag
    10921 tataatatta gcgtagttgc aaatattccc aaagatattg aaattggagt tttaaaattc
    10981 atcaaaggag ctaacataaa tgatagtggc attattatgc ctaattttat aaactcctta
    11041 agtactttga atacacgctc tttatgtggc atataatttc taaagaaaat cgcaaacatg
    11101 gcaattaccc aagaagcaga tattacgcct acaaagccat ttataatagt tgaccttatt
    11161 gaagatatag acactatcct ttcataaagt gattttagat agctaatatt gtcctcttta
    11221 tttactaaag tataacttct acctaccatc tctttatttt caagaccaaa tgtacctagt
    11281 atatctactc caacatctaa attggcaaca attccagcct ttcttgttgt ggcagattcc
    11341 agtagacctt tcccttctcc actaaatttt attattggag aaagtcttct cttattctta
    11401 taatctaggt cacttggaaa tgcacttgct atataaacag tgtcattttc tccaaccata
    11461 gaaaatactt gttcaagata tttatttata ttttttacaa tatttttctt cattgtatca
    11521 taagtattat catttagatt taatctatac atatctaatc tatatgtatc tccaagttct
    11581 acaaatatag catcactatt tttataggat tttttagttt catctagtaa tttatcataa
    11641 tctgtagata ttccaaatgg cattttagaa tctttcttat taatattatc aatattacct
    11701 gaatcaatcc ttccatattg gtccatagct actaaacata agtttctgtt tttaactaat
    11761 tgattatttt caattatatc agcatttcca attacagatg tctttaaacc attttctgat
    11821 aaagtttttc ctaatgaccc taaatttgct ccatattctc catactcaag attttcattt
    11881 atagatctat taatgcctaa atcatttatt tctttagctt tttgacctgt tgcgacttca
    11941 aaagctttag cagtatcttg gtctactttt tgaaaattga tatagtcttc agtagttaca
    12001 ctagctcttg ttcctgcacc catacttgca caagaccttc tatcatcagt accttgatcc
    12061 cctcttatat tcatgagagc tacatagcct ctcttttcta tctcattttt caagattgga
    12121 atattcatca tacttccaag attggtccta ttcatatcaa taaatatgac cttgcctttt
    12181 tcttcagcaa aaccatagct agcagttgat acaaaaatca taatgactat tgcaaacaac
    12241 gacataatct tttttctcat ttatttgacc tactttctat tcagatagtg cgtgattact
    12301 tcttcaagta tctcatctct ttcaaatttt ttaattatag ctcttgcatt tttatgacta
    12361 gttatatagc tagaatcacc agaaaggaga taccctataa tttgatttat agggttatat
    12421 cctttttcaa ctaatgcttt atatacaaaa tcaatagtat ctcctacact catcctatct
    12481 tctggtattc cttcaaattt catagtgtag tctaaatctt tttccaaact aaaaacctcc
    12541 ttgagtgaat gttatttaac ttgtgtctta ataacctcac ttgcatagct taaagcttgg
    12601 tcaaccttag atacatcact agctcctgct tgtgccatat taggtcttcc tccacctttt
    12661 cctccagcta tttttgcaac ttctctaaca atattacctg aatgtattcc cttatctaat
    12721 acatctttag tggcagtaac tacaaagtta actttatcat ctactacatt tgcaagtact
    12781 actacaccag aaccaagctt atctcttaga ttatctgctg tttctcttag tgtatccata
    12841 tccatacctt caaatttatt agttatcaaa ttaactccat ttacttcaac tttagaatca
    12901 aaaatagaat caactgattg taaactcatc ttagctttca tatcatgcaa ttctttagat
    12961 aagtttttat tttcttctaa taaagaactt attctttgaa ttaaattatc ttctttagat
    13021 tttaagttta cacaaacttc agagataatt ccatctcttt cttttaggta ttcataaaca
    13081 gcttttcctg ttattgcttc tattcttctt acacctgctg ctactccacc ttcagataat
    13141 attttgaaca ttcctacttg agaagtattt gtcaagtgag ttcctccaca aagttctgta
    13201 gaataatctc ccatagaaac tactcttacc tcatcaccat atttttcacc aaatagtgct
    13261 gtagctcctt tttctttagc ttcctttata ttcataatat cacattttat gtctaaagat
    13321 gataaaataa cattatttac tttttcttct attactttta attcttcatt tgatattgct
    13381 tcaaagtgag taatatcaaa tctaagtctt tctggagtta ctaaagaacc agcttgatta
    13441 acatgttctc ctaatacttc tcttaatgcc ttatgaagta aatgtgtagc actgtgattt
    13501 cttgcagctg acattctagt ttctctatct acgagtgtct ttactttgtc cccattactt
    13561 attcttcctg attttattat acctatatgt tttatactat tattagcacc ttttttagta
    13621 tttaatactt ctaaaactaa gttttcatta gttatcaatc cacaatctcc aacttgtcct
    13681 ccaccttcag gatagaaagt agtgttatct agtactatag ataccttatc gccttcttct
    13741 gcactttgaa ctagctcatc atctttaact atagcttcaa tagttccttc accataaatt
    13801 tcactatacc cattaaatgt actgtcaact gtagattcta attttgatag agggtcttcc
    13861 ttccaacttt ctccatccat atttcctcta gcatttctag ctctttcttt ttgcttttcc
    13921 atttcttcat taaaagcttc ttcatctaca gatagatgtt cttcttctaa tatttcttta
    13981 gttaagtcta tagggaagcc ataagtatca tatagtttaa aagcttcttg accacttagg
    14041 acagtctttc cttcattttt taatacttct atataagagt tcaatatttc tgttccttgg
    14101 tctatagttt cattgaattt ttcttcttct attccaacta cttttttaat ataactttct
    14161 ttttcaacta aaactggata tgctttatca tttactttta taacctcatc tattagtttc
    14221 tgtaaaaata attcttttac tcctaataac ttaccatgac gagcagcacg tctaaggagt
    14281 cttctaagaa catatcctct tccttcattt gatggaagca ctccatcact aactaagaat
    14341 gttacagctc ttatgtggtc agttattatt cttattgata tatcattttt tggattctct
    14401 ccatatttta ctcctgttaa cttctcaact gcttctagta tagatttaat tgtatcaact
    14461 tcaaagatag tgtctacgcc ttgcataata catcccattc tttcaagacc catacctgta
    14521 tctatattct tgttttctaa tagactataa tttccttctt catctctatc aaattgagta
    14581 aatacatggt tccaaaattc taaataccta tcacaatcac agcctggttt acagtctgga
    14641 ttgtcacatc catattcttc tcctctatca aaatatattt ctgaacaagg accacaagga
    14701 cctgtaccta tttcccagaa attatcatct tttccaagtc taaccattct ttcttctggg
    14761 aaattcattt cttttgccca tatatcataa gaatcatcat cttcttcata tacagtcacc
    14821 catatcttat cttctggtat atttagccat tctgtaacaa attcccatcc ccatttgatt
    14881 gactctcttt taaagtaatc tccaaatgag aaattaccca tcatttcaaa gaatgtagca
    14941 tgtcttgcag ttatacctac attttctata tctcctgttc ttatacattt ttgagatgta
    15001 cacattctca cacttggagg tacttctaca cctgaaaagt agtttttcaa aggtgccata
    15061 cctgagttta taagtaacaa actcttatca ttgtttggta ctaaagaata actatttgct
    15121 acataatgac cttttgactc aaaaaattct aaaaacttac ttcttatttc atttaatccc
    15181 atcttttcca tatcatgttc ctcctaataa attttatttt ttcgtttaaa taaaaaacct
    15241 cgtccctata aataaattta taggggcgag gacaatcgcg gtaccaccct aattatcata
    15301 caaagtatga tatcttcgga aaaccttagc tataacgtaa ctaccgtcaa tctctactta
    15361 aatcttcttt ataaaagata aattgttcaa gattgctact aaaaggctgc ttcaaatatc
    15421 atattctaag ggcttccagc atttccctct ctctgtaaga catcaaatat tttactattc
    15481 cttatcactg tatttgtttt ttaattaata tactaattat attactaaat tttacaaaat
    15541 taatcaatac tatattttca tatacaataa atttatttta taaactttat ttactcttaa
    15601 tatactttat tgcttatttt tttaattttt tagatgtttt tataaacttt tctcgattat
    15661 acctccacct actacaatat ctccatcata cattactaca gattgacctt tagtaatagc
    15721 tctttgagct tcatcaaaaa ctattttaat agtatcttct cctactctat gaatagtagc
    15781 tggtgatggt tttgctgaat atcttatttt ggcttgaact cttaagggtt cttctaacgt
    15841 atctattgat ataaagttaa catctttagc aataagttcc tttttaaata aatcttcatt
    15901 gtctccaaga actatggtat tatttattgg atttatatct attacaaaca ttggttttcc
    15961 aaaagtaatt ccaagccctt ttctttggcc tatagtataa tataatatac ccttatgttt
    16021 tccaagtata ttgcctttag tatctacaaa aaaaccttct tcaatacgct ttttagaatg
    16081 tttttttaca taatttgcat agtcattatc tttaacaaaa caaatttctt gactatcagg
    16141 cttattgtgt actgccatac ccatttcctt agcaatttct cttactctat ctttttcata
    16201 gtcacctatt ggcaataaag tatgctctag ttgctcttgt gtaagattat aaagtgcata
    16261 agtttggtct tttttatctg taacagattt ttttagcaaa tatcttcctg tactttcatc
    16321 cttttctatc tttgcatagt gacctgtagc tacataatca caacctattt gtctagcctt
    16381 cttataaaaa tcatcaaatt ttatatgttt attacaagct atacagggat taggagtttt
    16441 tccttccaaa tattcatcta taaaataatc tattactttt tctttaaaca cttctctaaa
    16501 atttaaaaca taaaatggta tacctatttt atttgctacc cttctagcat cttcaacagc
    16561 agataaagag caacaaccac cttcaatttc aacaacatca tcatcttgcc acaacttcat
    16621 agtaacacca atgacatcat atccttgttg ttttaaaaga tatgcagcta cagagctatc
    16681 aactcctcca ctcataccta tcattacttt tttattcata aacaaatttc acctcatctt
    16741 ttgcattact tattattata ccatatagag caagtattgt acttatttaa aatatagctt
    16801 gttctatttt ctaaaaaaat aatcatcttt attatttgaa tcttatggat tattttgtta
    16861 actaattaac ctctaaatca agttaaaata cttaatttag gcaataaaaa atagccattt
    16921 taaaaacagc tattttttat gtcctaatta tatcattagt acttcttttt aatcattgta
    16981 aacttctcat aacacaatca cttattttta ttcttcttct tcaacatcat ggtcatgagc
    17041 atcatctata acatatccat ccaattctgg tatatggata ttgttctttt gagcataatc
    17101 tataagggct gcttttacag cttgttcagc taaaactgaa caatgcatct ttactggtgg
    17161 taaaccatcc aaagcttctg caacagcttt atttgtaagt tctaatgcat cttttatagt
    17221 ttttcctttt atcatttcag ttgccataga agaacttgct atagctgaac cacagccaaa
    17281 tgttttaaac ttaacatcct ttattacatc tccatctatg tctaaaaata tcttcattat
    17341 atctccacat gtagggtttc caacttcacc tacaccactt gcattatcaa tttctcccat
    17401 atttcttgga ttcataaaat gttccataac tttatcacta tattgcatta tcttttcctc
    17461 ctactcaatt tatgtctatt ttttatgctt tatctttatg tctattttac taaaccttgt
    17521 ttcttcgcat catcataaag tggtgacatg cttctaagtc tttctattac ttttggtaag
    17581 ttctctagta tataatctat atcatcatct gttgtaaaat ctccaactgt aagtcttaat
    17641 gaaccatgtg ctatttcatg tggtaaacct attgcaagaa gtacatgtga agggtctaat
    17701 gaaccagaag tacatgcaga accacttgaa ccagcaattc ctaacatatc taataataaa
    17761 agtattcctt caccttctat aaattggaat gcaaagtttg catttcctgg caatctatta
    17821 tctaagctac catttactct tgtgtaaggt attctttcta acacaccatc tattaactta
    17881 tttctaagac gagttaaagt ttctacatga ttttgcatat tttccttagc taattctgct
    17941 gcttttccat atccaactat tgcaggtata ttttctgtac cagctctttt gcttttttct
    18001 tgtgctccac catggacaaa gttatgaagt cttacacctt ttcttatgta taaagctcct
    18061 acaccttttg gtccatatat tttatgtgaa gacatactca tcaaatctat tcctaattct
    18121 ttaacatcta ctggaatatt acctgtagct tgtactgcat cagtgtggaa taatatctta
    18181 tgttttttag ctatttcgga tatttcttta ataggctgta tagttcctat ttcattattt
    18241 gcaaacatta tgcttataag tatagtagta tcttttattg aatcttctag ttgctttaag
    18301 tctacttttc cttcgctatc aacatctaaa taagttattt caaatccatg atgttttgct
    18361 aagtattcac atgtatgaag tattgcatgg tgctctattt ttgaagttat tatatgatta
    18421 cctttgtttt tattagcata agctacaccc tctagtgtcc agttatcact ttctgaacca
    18481 cctgctgtaa aatatatttc actaggttca gcatttataa gggctgctac ttgttctctt
    18541 gctttatcca aagcatcttt tgcttctctt ccgaatgtgt gaaaactaga tgcattacca
    18601 aaataatctg ttaggtatgg catcattgca tccaatactt cttttttaat aggtgttgtt
    18661 gcagaataat ccatatacaa tcttcttttt tccattacat tcatctccaa atctattttt
    18721 taatatttgt tatatcattt tctaatttat ttttgttata gtcatttatc atatctttaa
    18781 gagtaattga atctataaca tcttctatac cctttttcat cttttcccaa actatttttg
    18841 ttacacacat gtttgagttt tcacatacat cttcatctag tacacagtca gataatgcaa
    18901 ctggtccttc taatactaca agtatatctc ctactgttat gtcttcagca ttctttgaaa
    18961 gcaaataacc tccttgagca cctctcacac tttttactag acccgatttt tttaaagatg
    19021 aaaatatttg ttctaagtac tgatctgata ttttttgctt cttagcaata aattttaatg
    19081 atactggtcc attgtcttga tttaatgcta attcaaacat tgcttttagt ccatatctac
    19141 ctttagtaga caacttcata tattatcacc tctatataga aatctaattc atactgtttt
    19201 agtaggaatt actttcaaga taattttatt ataccctact aattttgtca agattaattt
    19261 taatttttta ttttgaataa attccccatt ttccaatgtt ttcaaatcct agttttttat
    19321 atatctttcc agcctcttca ttatcataaa acaaacaagg aattttgttt tctctcagta
    19381 actctgaaca tagtttaatt aggcattttg tagcaagtcc tttagctcta tatttaggat
    19441 gtgttcctac tccaattatc attgcatgag tcctattttc tgaagtactc tttgccattg
    19501 acaccacctg tttgttaatc tctatacaat atcctctacc tgtccttagt ccatttttta
    19561 aattctctaa agttgtactg tgaaattcat ctattaagtc ataaagtttc actgtctttt
    19621 ttagatttcc aagcctaatt cttttaacct ttgcattaca attattatct atcaaaaact
    19681 ttttagtttg aagtttgcaa aaatcaacta cctttaaatt attaagtcct aatctctttg
    19741 ctaaagcatc tacagcacat attttcccag atatttcttc gtaattagta ttacgaagta
    19801 gattataaaa accttcaaca tcatactctc catcagaata aaatatcata aattcaaaat
    19861 atttaagtaa tattgccttt atttcaccat gttcacccac atctgcccat atatttaaaa
    19921 aattatttcc atatccatat ctttcaatat cccctattat aaacaaattg tactcaggtt
    19981 ctttttttaa gtatttcata actctactgt gataagttga gttaagtttt ataatcataa
    20041 tatctacccc cgatacataa agttcagtct aaaaatcact atttagatat tctaaatatt
    20101 tttttatatt tttctcagtt ttattttctt gtggtatgta ataaatacta tcttttatgc
    20161 tatctggtaa atactgttgc tttacataat gatttttaaa attatgagga tatttgtatc
    20221 cacaacccct acctatttct ttagaaccag aatagtgaga atcctttata ctatttggta
    20281 tatccttaac aaactcttta tccagttcat ccatagcttt cattattgct atacatgcag
    20341 aattagactt tggagatgta gccaaaagaa tagtagcttc tgctaatggt attttcgctt
    20401 ctggaaatcc aagttgcata gctgagtcta cacatgcctt tacaattaca atagcatttg
    20461 gataagctaa acctatatct tcactagcta ttactaaaag tctcctacat atacttatca
    20521 aatccccacc ttttataagt cttgctagat agtgtataga tgcctgtggg tcacttcctc
    20581 ttatagattt ttgaaatgca cttaaaatat cataatgact atcaccattt ttgtcatagt
    20641 ttataatttt attaaatgta gatgccctta ttacatctga atctatataa gttatattat
    20701 ccttatttgg tttagttgaa tagactacca cttctaaaat atttaaggct cttctcatat
    20761 ctccatcaga aagaattgct atatcttcaa tagcattatt attacactca atatccatat
    20821 agctatcttc atttaatact tcaacagctc ttttcagtcc tttttctaca tcaagttttt
    20881 ctaaaggttt aaattcaaat acagtagacc tacttaaaag agctttatat acataatgat
    20941 atggattttc tgtagtgcta gcaatcaaag ttatactccc attttccata aattctaata
    21001 ttgattgttg ctgtttttta ttaaaacttt gtatttcatc tatatataat aggactccat
    21061 ttacattatt tatacttcca agctctgata tcaccttttt tatatcttct aaagatgaat
    21121 tagtagcatt tattttatag aagcttttat tgcttctttc tgctattatt tccgctactg
    21181 tagttttacc aacaccagga ggaccaaaaa atatcatatt tggtaaaaaa ttagattgta
    21241 atatcttaga caatattttt ccattaccta ttatatgact ttgacctatt acatcattca
    21301 ttgtttttgg tcttatttta tctgctaaag gcattttctc actccttttt aatatactta
    21361 tcattacctt aataattcgt ctaaaaaagg tatctatttg aaatagatac cttttctaat
    21421 ctaaacctat aaaatataga ttattgattg ttttttaatt tttttaattc atctgtaagt
    21481 ttgtcattaa gtattcttat atgagtacct ttcataccta atgatcttga ttctatcaca
    21541 cctgcactct caaattttct aagtgcattt actattacag acctagttat accaactcta
    21601 tcagctatct tacttgctac aagtagacct tcttttccat ccaattcagc aaaaatatgt
    21661 tcaactgctt caagctcgga gtaggacaga gtgcctattg ccatttgaac tacagctttc
    21721 tttctcattt cttcttccaa ttcttcacct attgctctta atatttcaag accaacaaca
    21781 gttgcactgt attcagctat tactaaatca tcatcattga atgaatttga atatcttgaa
    21841 agtaccaatg ttcctaatct ttgaccgctt cctaatattg gaactactgt agtatatttt
    21901 tgtaatcttc catgttcttc aggaaagatt tctaatatct tctcaccatt taaattttct
    21961 aatgtttcat caatttttaa cacattttga gtatattcat ctgaaaattt cttttgctta
    22021 gtatactcat cttctataac tgaactgtct tgaacatcat ttaaatgaag acctagtact
    22081 ttaccttttg cacttactac ataaacatta gaacttaaaa cgtcgcccaa tgctccggcc
    22141 agtaaatcaa aagagacact gcttccacca cttgtttgta atgttttatt tattttcctt
    22201 gttttttgta acacttcact tgccatttaa tcatctcctc atgaaattta ggccttttaa
    22261 taacctttta tattttctaa tattattttc acatttaatg aaaatttatt tttctattag
    22321 ttgtttacac ttgaaagatt atcatagatt ggaacctttt tcaagagaat tatgtgattt
    22381 ttttcaattt tcactctttt ccgtcattat gctcttttgt ctctttatta cattctttat
    22441 tagaacacac tgtttttgtt tcagatttag tcactttttc aaccatatat gaaccacact
    22501 tctcacaaaa ctctcctgtt ggcttattcc aagctacaaa gtcacagttt ggataattag
    22561 aacacccata aaaagcttta cctcttttag attttctaag tataatatct ccttcttcac
    22621 attttggaca tttaacacca attttattca taattggtct agttgtttta caatcgggat
    22681 agtttttaca agccataaac tttccaaatc taccatattt gataaccata ttagacccac
    22741 agttctcgca aatctcatca gtttcttcat ccatatttac tttttctata ttttcaagag
    22801 cttcttcaat agcctcttta agtggtgcat atgattctga tacaatcttt ttccaatctg
    22861 tattgccttc ttctataaca tcaagtttac tttccatatc agcagtaaaa tctacatcta
    22921 taaatttatg gaaattattt tccaaaatat cagtaactat tattccaagt tctgttggac
    22981 atagacttgt tccttgcttt tcaacatact ctctattcag tatagtagct attgttggag
    23041 catacgtact tggtctacct atacctagtt cttctagtgt ttttactaaa ctagcttctg
    23101 tatatcttgc tggtggttgt gtaaaatgtt ggtctggcaa tatattttct atatttaata
    23161 tatctccttt ttctatactt ggtaaaattt tgtcttctct ttctgaaaaa ttatatacct
    23221 tggtataacc atcaaatttc atttttgaac cagttgcctt aaatacaata tcgcctattt
    23281 tacattctac atttagtata tcaaacactg aatcttccat ttgactagct acaaatcttc
    23341 tccaaattaa attgtataac ttgtactggt ctttgctcaa tgaggctttt atactctcag
    23401 gagttctatc aactgaggtt ggtcttattg cctcatgggc atcttgaacc tttttagtat
    23461 cctttttttc ttttttatca ccagtgtttt tatagtagtt ttctcctaaa ttgtttacga
    23521 tatagccctt agctttttct tttgcttcat cagatattct tttagagtca gttcttatat
    23581 atgatataag accaacagta ccttcacctt ctatgtcgat accttcatat aattcttgtg
    23641 ctatcatcat tgttttttta gtagtaaaaa ataacttatt tgctccttct tgttgaagca
    23701 tacttgttgt aaatggtttt ggagctgatt tacgtcttga tttagattct atcttctcaa
    23761 cctttaaatc tttaccttcc atttctttta gtatttcatt tacagcttct tcattttcta
    23821 attcaatctt ttcatcattt ttaccataaa atttcaaagt tatatctttg tcttttactg
    23881 tcttagcctc aacatctatt gtccagtatt cttttggaat aaatgccttt atttcttttt
    23941 ctctgtcaca aattaacttt gttgtaacag attgcactct acctgcacta agaccttttc
    24001 taaccttttg ccataatata ggacttattt gataacctaa aagtctgtct agcactcttc
    24061 ttgcctgttg tgcatcaacc aaatttatat caatatttct aggatgttta attgcttttt
    24121 taattgcatc cttagttatc tcattaaact ctattctaca atcctcagat tcatctaaat
    24181 ttagtatatg agctaaatgc catgatatag cttcaccttc tctatcaggg tcagttgcta
    24241 gatatacttg tttagatttt ttagcttctt tttttaattc ttttattaca tcgccttttc
    24301 ctcttatatt tatatattga ggttcaaaat tattttctat atctacacct aatttacttt
    24361 taggtaaatc tctgacatga ccaactgatg ctttaactgt gtaatgactt ttccctaaaa
    24421 atttttctat agttttagct tttgcaggcg attctacgat gactaatgtt ttggccataa
    24481 aaccatacac ccccactacc taatcttaat ttatacattt aaactgtacg ttttattatt
    24541 catttctact actaactcat ttagtactaa ttcattgatt attgaattta catactttat
    24601 ttctatacca gtataatcac aaatactatc tatatgcaag tttccttctc tttttatagc
    24661 ttcaattatt tgcttagact tggcatttag acttatatta tcatattttt gatttatttt
    24721 tttatcaata atattaaata tgttatattc atttaaaata tcatctatat tttcaattaa
    24781 ttttgcacct tcttttataa ttttatgaca acctctactc atttgagaat ttatattacc
    24841 aggtattgca aaaacatttt ttccatgttc aagagcaaaa tccactgtga ttagtgcacc
    24901 actttttatt gctgcttcta caacaactac tccatcactt aaaccactga taattctatt
    24961 tctattagaa aaatggtacg gggctactgt cgagttaata ttatactctg ataataataa
    25021 tcccccattt tctaatattt tatttgataa atgtaaattt tgttttggaa gaggattgtc
    25081 tactcctgaa ccaagtactg ctatagtttt agattttcca ctcatacaac ctatgtgcga
    25141 gtatgcatct attcctatag ctaatccact tactatatta actccatatt gggatagttg
    25201 acaacttatt gtttttgcac aattaattcc ataggctgta ggttttcttg aacctactat
    25261 agctatatta aaattattat taactaagcc tatgtcacct ttgtagaata ataactttgg
    25321 agcgttgtat atattcttta aattttcagg atattcttta tcatatttac aaatatattt
    25381 aatcgctttt ttgtaaagta gttcttttat attttctaaa taagcgtgac ctctatattt
    25441 tactatattt tttctaatat tcaagcttat attttttaaa ttatatattt ctttttcaga
    25501 aaaatcaaat atatcttcaa tatttactat ttcattttct attatttcaa ttgtttttgt
    25561 tgttattcca ccaattgatt ttaaccataa ataagtatct cttttttcca taaaatccac
    25621 ctcaaaaaac tatttaaaat aactataata agcctttcta aatgaaaaag cttctataat
    25681 acactgtgaa tttatcaagt ctctttcttc taaatctgct attgttcttg ccatttttaa
    25741 aagttttgta taacttctac tgcttaggtt gtatttatta aatatcatct cagccgtttt
    25801 tgaagcttct ttttccagtt tacaatattt aaataaattg taagctttaa tttcactatt
    25861 attttttata ttatccttct taaatctatt tatctgtatt tttctagcat tttctactct
    25921 tctctttatt ttttgagagc tttcttcttg ttttaaagaa ttaaaatctg aatattttat
    25981 agaattaact tctacaaaaa tatcaaacct atctaacaat ggacctgata ttttatttat
    26041 gtatctatca atttcgttac ttctacatct acactctgtc tctgacatat aataaccaca
    26101 tggacatgga ttcatagctg caactaacag tactctacaa ggatattttg cactgtactt
    26161 tactctggat atatttacat atccatcttc tataggttgt cttaaagtct caagtatttt
    26221 tctatcaaat tcggctatct catctaaaaa taagatacct ctatgtgcta aagcaatttc
    26281 tccaggtctt gcatccattc ctcctccaat taaagattgc tttgtagttg tatgatgagg
    26341 tgctctaaaa ggtcttttat caattattcc tttagtttca tttatcatac ccaatatact
    26401 atagacctta cttacttcta tcatttcttc tacacttata tctggtaaaa ttgtccttac
    26461 tctttttgca atcatagttt ttcctgagcc tggaggtcca atcataaaca tattatgatt
    26521 tccagcagca gcaatttctg cactcctctt tacaaaataa tttcctttta catctttaaa
    26581 gtcttcatca tatttacttt cttcattatt aatcttagta tatttgcaat ctatataact
    26641 attatctttt tctaattctt cgttctcttt tcttgactta tcatccaaaa aagacattat
    26701 ccttacttta tcgactttta tctcttcgtt taaaaaatcg acacattctt ttaaactttt
    26761 aataggaatt atatcaattt catctacaaa tagactttct tttatgttct caattggtat
    26821 aaaaatacgt tttatgttct gagtttttgc acccataatt atgggcaata tacctcttac
    26881 ttttcttatt ttcccatcta atgataattc tcctataaac atactttctc ttatgtaatt
    26941 ttcatctttt tttattaact ctcttaaaag gcctatactt atagataaat ctaaaaaagc
    27001 tccttctttt tttatatctg ctggtgataa atttacaact attctcgtgc ttggaaattt
    27061 atatccacta ttaagaattg ctgactttac tctttctctt gcttctttaa tttctttgcc
    27121 tggaagacca acaatattaa aactaggtat tccattgctt acatctactt caaccttaac
    27181 taaaaagctg tcgattccta ctaagttgct tgaattaata atacttaaca tatttaatcc
    27241 tttccaaaaa cattatcttt tttaaaaagc attcattatg tgatttattc ttttttctaa
    27301 taaatatacc tctataacat caaatcttac gggagtgtta tttaagttat tttttataat
    27361 ataataattt gcaactccaa ttatcttttt tctttttttg aagcttacag attcacaagg
    27421 gtagccaaac tttatgttag accttgattt cacctccaca aatataattt catcttccat
    27481 ttttgctatt atatctattt cccctatctt taatctgtaa tttttttcta gtatctttgc
    27541 tccttttgat agcaaataat taactgctac tttttctcca aaatcacctt tttctttatt
    27601 attcattatt cactccaata cattaaattt tcacttttaa attattgtca gtatacaatt
    27661 ttactattca agtttttgga ttgattttat ttccatcaat attaataaat acttttattg
    27721 actttattct tttatgatat tattctagtt ttcatagtaa aagtttattt ttttggagga
    27781 gtgcctttaa tgaacagaga aaaattagtt gcttttatac gatatttatc tttatttata
    27841 ttagttgtaa gctatttgaa aaatgttgaa aatacattgt atccggtcat aactatgata
    27901 atattatttc taattataat aaataatcaa gttagatttt tttctttatc aaatgagaat
    27961 aaatttatac ttatttctta ctttttagaa cttatattaa tttttatact ttgtaattat
    28021 acaaaaactt ttaattcgat ttactttgtt cctttaatat tagatatatc atttttaata
    28081 aaggaaaaat ataagtacat attgttctct tttgttataa tatcttctct tattattagt
    28141 ttagataaaa atatatattt ggcactggag tcaagctcta tattgctaat tataactgta
    28201 ctttctatat atatagaaaa tgagaattta tcaaaattat acagtcaaaa tctttatgat
    28261 aagcttagaa tttctaaaga tgaattaaaa aaagttaatg ctgatttgga aacttatgca
    28321 agttctatag aagaattagc aattttaaaa gaaagaaatc gaatatcaag agaaatacat
    28381 gatagtgtag gacattctct ttctacaaca ataatacaat taaatgctat agaaaaactc
    28441 ttaaaagaca aacctctaat ttatgactta gtacatgaac ttagagaatt tgtaactgaa
    28501 agttttcaag atgtaagaag ggctatatct gagttaaaac ctgttgaata tgaaaattac
    28561 caaagtttat ttaaaatcaa agaattagtt aaaagcttta ttaagcttac aaatattaat
    28621 gttaaattaa ctatttcaaa aaatacttgg aacttatcca gaaatcaatc tataacttta
    28681 tatagactta tacaggaatc cctttccaac tcatctaggc atggaaaggc aactgaaatc
    28741 agaattttta ttacattcaa tacttcaaat cttataatta ctattagtga taatggtata
    28801 ggatgtggca atataaaaaa aggaaatgga cttaatagta tcaatgagag aatatatgaa
    28861 ttaaatggta aagttgaatt tgataattct aataatggtt tcacaattag agcttctttt
    28921 cctaaatttt cagggggtga tttttttgaa taaaattaaa gttcttatag tagatgacga
    28981 aaaattaatt agaaaaggtc ttaaaattat attatcatct tataatgatt tagaaattgt
    29041 aggagatgct tctaatggat atgaagcttt agaattttgt aaaatgaatg atgtagacat
    29101 agttcttatg gatatacgaa tgaaagtatg tgatggggtt ctaggtacaa ggcttataaa
    29161 ggaatataat aattctataa cattattaat tttaacaact tttaatgatg atgaatatat
    29221 aaaagatgct atgaagtttg gtgcttcagg atacctttta aaagatagct ctgatgaagt
    29281 attgcatgaa gggattcgtt cttcattttt tggcaatatt gtgcttgata aaagtgtagc
    29341 tgaaaaaatc atgacctctg aaaaaacggt taaacaagag catctttatg acatgtacaa
    29401 tttaacagaa aaagaaatat ctataattag actaattgca aatggactta ataataaaga
    29461 aataagtcaa gaactattct tatctgaagg tacaataaag aataatataa ctaatatttt
    29521 agctaaacta gaacttcgtg acagaactca actagcaatc tttgctttca aaaataaaat
    29581 tgtaatagaa taaatctaaa aatataaagt gacttaagtc acttttttta gtgacttata
    29641 gtaatagaat tataattcac aattctttat tataatatta tagcttttta acttatacaa
    29701 aattattttg gagggattac tatggatata gtcagagtaa ataatataac aaaaagattt
    29761 aatgataaat tagtacttga taacatcagc ttttctgtta aaaaaggtga gatatttgga
    29821 cttattggcc caaatggtgc aggtaaatct acacttataa atataattac aaatcttatg
    29881 ttaccaaata gtggtagtat tcaaatcaat ggtttagatt tatcaaaaga ttatataaaa
    29941 gcaaagtcta taatagggtt agttccacaa gaattagcaa ttatagaaac tcttactcct
    30001 tttgataact tagagtactt tggagctttc tacggtttaa agggaaaact acttaaagaa
    30061 agaatcattg aagcattaga agttacaggt cttactgaag tcaaaaagaa aaaggtcaaa
    30121 aaattatctg gtggtatgca aaggagatta aatattggaa tagcactctt aaatcatcct
    30181 aaaatattaa tattggatga accaactgta ggtgtagacc cccaatcaag aaatcatatt
    30241 ttcaatttta taaaagatat atctaaaaaa catgaaacta ctgtaatata tacttcccat
    30301 tatatggaag aagttgaaca cctttgttct aaaattttta ttatggatga aggtaaagaa
    30361 attgcttttg gagataatga ttatttaaaa tcactcgtat ctacaaatac aaaattaatt
    30421 atagaaataa aaaatataaa tgcccaactt atctttgatt taaaaaatac taaaggtgtt
    30481 atatctgttt tagaaaataa ttcattatta gaattggata tagataaaaa attacaactt
    30541 acagatatac tctctgttat tgacaaaaac aattcaaaaa taatgaaaat ttcttatgaa
    30601 gaacctagcc tagaagacgt atttttaaat ctaacaggaa aaaatttaag ggactaatgg
    30661 aggtgatttt atgaaattat acatgagtgt aaaaactatg ctaaagagtt taaaaagctc
    30721 ctttatactt aatttaatat attttttagc tttgcctctg attttatctt ggtttcttgg
    30781 gatggttaca gaaagcatgt ttcaaaaccc tataaaaaca gaatctaccc ccattgttat
    30841 atatgacaaa gataacacaa gattatcaaa tgatttgaca aagtatttga aaaatgattt
    30901 atcttatata ttaacagtta aaaaagatga ttctaaggca gaattaaagc ttactatacc
    30961 taaaggctat gagagtagtt tattaaatga aaaatcaaat actctaaaca tagaaaaact
    31021 tggaactcgt gatgatattg caatattgct tcaagatata ttagatactt accatgaaaa
    31081 attttattta aataattctc agaaaataag ttcagaagat ttttctaaat tatttaataa
    31141 aaattctatt gatacttcta taataaataa caatgttaaa cagaactctt atgaatactt
    31201 tgctttagtt tcactaggat ttcttgtaat aatttttata atgaacaata ttttgtcaaa
    31261 ttatataagt gaatcaaagg gactttcaaa aagactttat tctatgccta taactagagt
    31321 acaattctta atttatgact ttgtaggttt atggatttat tcatttatat tcttattatt
    31381 atatgtttta ttctttagaa tacttggaat aacatttaaa ggtaattttg ccatattgct
    31441 cctactttgt gctttatcat cttattttat gacttcaata tctacctttg taaccagctt
    31501 ttttagtaaa aaatacggta cagcaatagt ttatgcatta ctctttttac agacaatttt
    31561 tggaggtatc ttcagtatga taagtgatac atttactaag cttacaagtt tatctcctac
    31621 ctatttaata ggagaattgt ttagtaatta tgaaacattt aaaactatag attcaatagg
    31681 agatttaatt ttcacatgtt taattatctc tacaatatta attgcacttg ctattgtaaa
    31741 ggaaaaatat aaatggaggg aggtttaata atgattagat taactattgc aaatataaaa
    31801 agatatctaa aaaatcataa tcttttaatt aatatggtta tgcttcctat tatactaatt
    31861 ttttcactta atttttttat caacaactca ggaaatcaat caatgtactt ttctccagta
    31921 gcaattgtat ctgactcttc aggaaagtat gaacataagt taataaatag ttctaaatta
    31981 aaagaaaatt catttagatt aaatgaacta gataaggcta tgaatctatt aaaaaataat
    32041 aaggtttctg ctgtattcgt tttagataaa aatttttcaa atagtataga taaattacaa
    32101 cgacctgtag ttaaatgctt taaaattgaa aatggtggtg gtagtctttg ggcagaatca
    32161 caaatagaat cttttattac taaatcgtta aaattgaaag tggataaaaa tatagatgat
    32221 aaattgacaa agacaaatat aattgataac aaaccagata agaataaagg ttcattttta
    32281 gtagtcttct tgatatgcta ttttatgtat attaatgcag cacatcttgc ttctgattta
    32341 tttacattaa aaaaatcaaa tgtgctaaaa agacttatct ctacagataa taaagatata
    32401 aaaataattt tcagtatatt tttaggacta ttttttattc aatcaattgt atacactttt
    32461 gctctattat gtttttcaat tatagaagac tttaatttga gtctgaatgt attgatgatt
    32521 gtactagcaa atagttttgt ttccactgga tttgtatttt gggtagctag agtatttaaa
    32581 aatgaaagtt ctataagtct tatatctaca ttttattctt taataggtct agctatatct
    32641 atctctagtc taataccatc aatggataaa ttatccttta tgactaatct ttcaaagttc
    32701 acaccatttt attggactat agatgctatt aaaaacaatg gtagtatttt aataaatata
    32761 atagctctaa tattaattgg aattatattt gtaactgcag gaagtcttaa actaagagat
    32821 tttgctaaaa actaataatt attaaatata attagtatct atttttatat agttgtaaaa
    32881 gaatatttca acatattaaa ttattttatc ttatattttt ctctttagtt tcacacatca
    32941 ccaagtctaa accttatatt atatattaat atttttgact tgtcaagtta tttatgttat
    33001 aatattaatc attagataat taaagcattg tacctaagtt gcaatatcta aaattcaatt
    33061 tggaggtata actagcatat gtataaaatt ggtgaaagtg taatgtatcc aaaagaggga
    33121 gcctgctctg taaatgatat tgtaacaaag aaaataaatc atgagatgca aaaatactat
    33181 gaattaagtg tcatttttaa tagtaacctt aaaatttcga ttccagttct aaatgcagat
    33241 agaattggca tacgtccagt tatggatgga aatgatgttg ataattttat ccaatctatt
    33301 aataaaacag atggtgtatg gatttttgat agaaaagaaa gacttaaact gtaccaagat
    33361 aaatttcatt caggagatgt ttttgaaatt gtaaaattaa tcaaaatgct tatgatacaa
    33421 gacagttcaa aacaattgtg tagcacagat aaggagtttt taaataaagc tcaaaaattt
    33481 gctttatcag aattagctgc tgctcaatgt aaatcctata caatggtatt agaggaaatg
    33541 aaaaaacata tattaaattc aaagaataca aattaaacca gttttttaaa ctggttttat
    33601 tttttatact aatcatccat gctactttag cttattttaa actgcctaat ctaatattaa
    33661 taaagttatc tcaattagat agatgtattt tattcaaata aaaaaaacta agagtaaatc
    33721 atattactga tttactctta gctaataaaa tttaatcttc ttcaagttct tttttctctt
    33781 gtaataatac acttaattca tttgcttcta aaacttgtgc ctctgcaata tcaagtaact
    33841 cttgttgcca ttccacacat ttatctttgt aatcaaaagg tagcgttctg tcaactatgt
    33901 acttatcaat acagtcctcc gcacttttaa ttatttcttt tctttcatca ataaattttt
    33961 ctaattctac taaggcttct tcataagctt ttctactcat ataaattccc cctttgtaca
    34021 attattctac attaaaatga tttttcctct tttttgtata aaattttatt tgtatataac
    34081 ttttccatca atatctttta tagtttgaca tccagttaat atcatagaac tacttagctc
    34141 tgattgaagt ttatttacat aagtttctac accatcagtt gcaccaccaa aagaagcagt
    34201 cacaaaaggt cttcctatta atactgcatc tgcacctaaa cctatcattt ttagtacgtc
    34261 taaaccagtt ctcactccac catctgcaag tattgtaact ttacctttaa cagcatcagc
    34321 aatttttggt aaaacttcac atgctcctgg tgtacagtca agcactcttc ctccatgatt
    34381 tgatacaact attgcatcca cacctgcttc tactgccatt aaagcatctt caacagtcat
    34441 tatacctttc aatataaatg gtagtttagt agactttact aactccttta tttgtttaac
    34501 atttttagga agtactggtt ttccatgtaa tgataaagtt actagaccac aggcatctat
    34561 atcaactcca actgcaaatg caccagcttc ttcagctaat cttatctttt ttataatatt
    34621 ttcattatcc caaggtttaa taaatacaat tccagctcca ttatactttt tgactacatc
    34681 taaattttcc attaaaaaag catctactgc cgtatcacca accattgcat aaataccact
    34741 attagcacat cctgcaacta ctggttctat gtaatctctt tcattaattt ttccacccat
    34801 atttaaagta gttcctgtta ctggagctgc aaatataggt gcactcattt tctttccaaa
    34861 catctctata gatgtatcag gatttgatac atcatgtatt actctcatat ttacttttac
    34921 tttttctaaa ctcttgctat tttctataaa agaagaacca cttcctttac ctcccatgcc
    34981 aggcacttca cctgcacaag caaccccatt acaaacctta caaactttac aacttccatt
    35041 taaattttct ctagcacttt ttaataattc attatattcc atctttaatc ccccattaac
    35101 tatagtaatt tatattatat acattatatc atattatatt tacagtatat ttttcaaaaa
    35161 actttttctg tggataggag ttatgccata tttttcaata gcttcatagt gttctttagt
    35221 tccatatcct ttgtgagatt taaacccata ttctgggtat actctatcat attggtacat
    35281 tatactatcc ctagtaactt tagcaagtat acttgctgct gctattgata tagattttga
    35341 atctcctttc acaattggat tttgagatat gtcaatacct ggtattgttg ctgcatcaac
    35401 taataaataa tccggtgctt tttttaaaca atttatagcc tttttcattg ccatatatgt
    35461 agcatttaat atattaaatt catctatttc ttcattgttt actataccta ttccataatc
    35521 tagtgcttct tcttttatta cttcaaaaag ttcatctctt tttgcctcac tcagtttctt
    35581 agagtcattt acaccttcta ttttggtatc ttttttaaat actacaacag aagctacaac
    35641 aggtccagca agtggtcctc ttcccgcctc atcaattcct ccaatatata aataaccttt
    35701 atcatagcct tcgttctcaa atatatttat agtttccaat ctttcttctt cttttctaat
    35761 attatccagc ttttttgcta gttttatagc tagaccttgt acagattttc tttcatcaac
    35821 tcttaacagc tctatatatt ccatatattt ttctacttct aaagtctcta ttatttcttt
    35881 aatctccctc acacttttat cctgcatacc attccctcat ctttcgtttt taaaaattta
    35941 atttttaaat atattaaaat tttttaatta gagtttaatt gcatccacta tagccacatt
    36001 ttaaacatat actacatcca cctgtatgtg caattccttc acttccacac tcaggacaca
    36061 caaatttttc atcttcttca aaatttttat ttattatttc tttggctttt tcatctttat
    36121 tatctttttc ttttttatta tttttataat attctttttt actattttct tttttatctt
    36181 ctttattatt ttcttccttt ttttcattta ttaaaattcc actcctttta catccatctc
    36241 taaatattgt tactccttta agaccagcct cccatgcctt aatatatata ttgaaaacat
    36301 cttcaactgt agtttcaaaa ggtatattta ttgtagatga tatagaagca tcaatatact
    36361 tttgccatac tttttgcatg tttattcttt cttcatatcc tatattcata gcagtaacaa
    36421 aaaaatctgg taaacattct tcatctgtaa tacctttctt gtccatatac tctttaacaa
    36481 ttggagtata tactttataa tacacatcct catcatgcaa gctttcagtt tttcttatat
    36541 atgaaagatt aaatataggt tctattccac cactaatacc tatcattgta gatatagaac
    36601 ctgtaggagg tatggttaaa agttgtgaat ttctaagccc atacttttta accatttcct
    36661 tagtgtcttc atttagattt tctataaaaa acttagattt agaaatattt tcatatttat
    36721 actcactaaa tgttccatat tctttagcaa ttagtgctga ttgcttcaca gcttcattta
    36781 acatagtctt accaattaaa tcacatagtt ctaaagattc atcagaacca tatcttattc
    36841 ccatttttat aagcatatca gctataccca ttacacctaa acctatctgt ctatacttat
    36901 gtacactatc tctttgttct tgtaatggat gcaagttcaa tccctcatct aagacatcat
    36961 ttaaagctac tacagcttct ctaacgcaag attttaattt attaatatca aaagtagcat
    37021 gttcaccaaa tggttgtatt ataaactctg acaagtttat tgagccaagt agacaacttc
    37081 ctccacttgg aagtggttct tctgcacaag gatttactcc tgaaaagcaa aagttcttat
    37141 cttcactcat aaaattatat gtttttattt tatcccaaaa caatatacct ggttctgcat
    37201 aatcccagtt atttttcaca aatttattaa ataaatcata agcatttact atcttaacta
    37261 ttttttctcc agtagtttca acttcaaaag tacaattata taactttttt tctcttacag
    37321 cttgcataaa ttcatctgtt actcttacag aaatatttgc tttagtaacc ttctccaaat
    37381 ctgtttttat atctataaag tcttctatct ctggatgatt tacatccata gaaatcataa
    37441 gagctcctct tctccctctt tgaccaatta agccagttgt catactgtaa agttccataa
    37501 aagagactgc tccagtcgta tgttttgcag cattgttaac tttcgctcct tttggtctta
    37561 gctttgaaat atcaattcca actcctccac cataactaaa agttctagca agtttttttg
    37621 cagtatcaaa tatatcttct atgctatctt ttggtggttc tattacataa caattagaat
    37681 atgttacttt ttttccttct ttctgtaatc ctctatttgc taaaattctt ccagcaaata
    37741 gaaatctttt ttgtcttatt agttttgata ttcttgaact attatttgaa attctcttta
    37801 tccattcatc aaaactctca ccattatgtc tatacttgtt cttccatatt gatttttgta
    37861 attcatcaaa ctcccaagct ttagtcctta tttcagagcg tctttctcta tataatatgt
    37921 agtgttttgc tatatctttt ctatttgatt ccataagtag tacttcaact atatcttgta
    37981 tttcttctac ggataagcat ttttccatag aattatagtc attagctatt tttcttgata
    38041 tatcatatgc caactctcta tctactccac cttcactatt tatactagct ttatagacag
    38101 catcttcaat cttactttta ctaaaaagtt cttttgtacc atctcttttt attatgtatt
    38161 tcatttttaa acctccaata aattagtatt aaattaatac acacattaac tataactcta
    38221 aacaaacttt ttatatattt gtatattttc atattaatta tctaacataa tatatattat
    38281 actattttat attcaatata acactcctta tagttttctt aacttgtaaa atatcttcct
    38341 aaaaaactca aaaataatat aaaaaagtat ataaaagtca aattatactc ttatatactt
    38401 tattttttta tatttttttc attttaactt tgattaatta ataagtattt agaaaataaa
    38461 ttctttattt tttctttaca aaaatatgta ttgatgtttt tatattaata ctaaacattt
    38521 ataatatata atttaa
//
LOCUS       DAEPIN010000033        38298 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.033, whole genome shotgun sequence.
ACCESSION   DAEPIN010000033 DAEPIN010000000
VERSION     DAEPIN010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 38298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38298
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.033"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            <1..469
                     /locus_tag="KR250_001899"
     CDS             <1..469
                     /locus_tag="KR250_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0067835.1"
                     /translation="ITSIGSIKSRIKIDTNRIEESRVENIKYLNKIGYLFLILMIIMT
                     TQFFTTLLSIKTKLSTAMNITTLLVIIYLIATYINSPNLKFNSSYSPEEDEKYWIAGI
                     MYNNPNDPSLMVNKRFGIGWTINFGNPLGKILYIAIALLLIFSLFSLIKSLLL"
     gene            589..1065
                     /locus_tag="KR250_001900"
     CDS             589..1065
                     /locus_tag="KR250_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBH0067836.1"
                     /translation="MLSKAEIKYFNECASEILSSEKVQLMRTFPHHGNVSCLEHSLSV
                     AYYSYLLCKKLHLSVDIQSVIRGALLHDFFLYDWHYKGDRKGLHGFTHPREALKNATL
                     FFQINEKETDIILKHMWPLTVKPPRYKEAFIVCLLDKFCCLVETLKIHSLLSPYHV"
     gene            1451..1918
                     /locus_tag="KR250_001901"
     CDS             1451..1918
                     /locus_tag="KR250_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HBH0067837.1"
                     /translation="MFKEMRLKKREMTKEDTVEVLKNGEFGTFSTISENGYPYGVAVN
                     YVYFNDSIYFHCARNGHKLDNISKNNKVSFLVVANESVIPDKFSTTYSSAIVFGKACT
                     VENEEKKNALVEIIKKYSKGFFEEGMKYIEKDMNLTTVVKIEIDHISGKASRL"
     gene            complement(1978..2550)
                     /locus_tag="KR250_001902"
     CDS             complement(1978..2550)
                     /locus_tag="KR250_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBH0067838.1"
                     /translation="MKVAVIFHSVCGSTYLLAREYKEALEEMNIEVDIFRVSDEVAKT
                     LPQYYLINSKEYKDEFESINVIKSGKEILDYDAIFMGSPTYYGNVSGPMKMFMDSFSD
                     IWVGTPLSGKIFGCFATAGSQHGGGELALQAMNIFAQHMGMTLLSVPCSVRGGYPAYG
                     ILHIAGDNSDIRPNDDAKIGIRDYLKRLNI"
     gene            complement(2613..3710)
                     /locus_tag="KR250_001903"
     CDS             complement(2613..3710)
                     /locus_tag="KR250_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HBH0067839.1"
                     /translation="MLLKSLTRKIEKKYPLNLAEDWDNVGLIVGDFDMDVKKVLVSLE
                     ANEDVIDEAISKNIDLIVTHHPFIFGKINKINSGDLKGRLIQKLIKNDISLYSMHTNF
                     DIAFDGLNDYFMEIMEFGNSKVLDITKSENLYKLAVYVPHNYSDELRKVLSNSGAGHI
                     GNYSDCTFSIEGEGQFKPLEGSNPFLGSVNDIETVNEVKIETVVPQKLLGGVISSMLD
                     AHPYEEVAYDLYKLENKGEIFGLGRISKLDKSMTLESLSRKIKEKLNMKHIRVVGNLS
                     TDITKVAVVTGAGSEFVKKAKRQGAEVLITGDVKYHEAQDALDIGMCIVDCGHFDTED
                     IFKNVMKRFLDEFSEIEVIKSNVYLNPFSTI"
     gene            complement(3724..4413)
                     /locus_tag="KR250_001904"
     CDS             complement(3724..4413)
                     /locus_tag="KR250_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HBH0067840.1"
                     /translation="MKLTDRLLKIASLVSDGKKIADIGTDHGYIPVYLLKEGKVPFAV
                     LADVNKGPLDNAHKEVIQNNLLDKVDLRLGSGIEILEIGEVEEVIIAGMGGILISELL
                     EAKKEVAHNVEKLILQPMQAQEELRYYLLNNGYEILEEVLVREDFRIYEIIVAKYTGK
                     NTIIEDEIYYEVGIKLLENKDSLFNDFIEKKIKTYSSIVNKLEGKNGEAIDKKRKESE
                     VAIKKLENLIK"
     gene            complement(4578..5744)
                     /gene="rpoD"
                     /locus_tag="KR250_001905"
     CDS             complement(4578..5744)
                     /gene="rpoD"
                     /locus_tag="KR250_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HBH0067841.1"
                     /translation="MENKSNKKELKKVTAKTLIEKGKKQGSLTLAEIMEAFSETELDK
                     DQVENLYETLGNLGIEITETKNYKADIDFSVADDDLSIGHLDEDAEAISHDDSSAIEI
                     ETVDLSLPKGISIDDPVRMYLKEIGKIPLLKPHEEVEFARRMHEGDEIAKQRLVEANL
                     RLVVSIAKRYVGRGMLFLDLIQEGNLGLIKAVEKFDYTKGYKFSTYATWWIRQAITRA
                     IADQARTIRIPVHMVETINKLIRVSRQLLQELGRDPKPEEIAKEMEMTEDKVREIMKI
                     AQDPVSLETPIGEEEDSHLGDFIPDDDAPAPAEAAAYSLLKEQIEDVLGSLNDREQKV
                     LKLRFGLEDGRARTLEEVGKEFDVTRERIRQIEAKALRKLRHPSRSKKLRDYLD"
     gene            complement(5762..7552)
                     /locus_tag="KR250_001906"
     CDS             complement(5762..7552)
                     /locus_tag="KR250_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HBH0067842.1"
                     /translation="MNDIKDIIEEIKARCDIASIISEYMNIKQSGANYKGLCPFHGEK
                     TPSFYINTSKQIYKCFGCGEGGDVINFVMKMENLDFMDAVKILANKCGIEINTNMNEE
                     TRIKIEKSKKFQDIHTEAARFYLSNLLGSKNLGYEYLRIRGLDDKIIKKFGLGFSLDS
                     WNSLMNTLISKGYKKQDLLECGLIAKNRDGTNCYDKFRNRVMFPIFDYRGNIIGFGGR
                     VLDDSLPKYLNSPDTLIFNKKQNLYGLNFARKNLESKTIVLVEGYMDLISLYQYGIKN
                     VVATLGTALTEQQGILIKRYADTAIISYDSDEAGIKATLRAIDILTKLGINVKVLDLK
                     DAKDPDEFVRKYGLSDYKKAMDVSTHYIKYKIDHLKKEFNIQKDEERVKFAKEASKII
                     KQLTSPVEIDFYTKYLSNQIDINVESIKREVYGKNYNKPYNNKNQKKIEEKVIEKVEV
                     RQDGKQLVEETLIKIMLEDKKIREIALLKVEESDFLLKESKEILNYMIKNQELDKITI
                     DKLKSLNISEEYLKELNSISLNSINLENTKEIEGIITNIRKNSLEEQINSLLREQQEL
                     ENNNDMKEVDGRVMEIALKIVEINKILKSL"
     gene            complement(7807..8592)
                     /gene="aroF"
                     /locus_tag="KR250_001907"
     CDS             complement(7807..8592)
                     /gene="aroF"
                     /locus_tag="KR250_001907"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HBH0067843.1"
                     /translation="MKENLFNNNSKIEINTNNKKITVKKDKLIIAGPCAIESYEQLLE
                     TAKFVKSQGANILRGGAYKPRTSPNSFQGLKKEGLEILKAVKDEVGMAVITELMDVRD
                     MDELYSISDIIQIGSRNMQNFTLLSEVGKQNKPVMLKRGIASTITEWIGASEYIAIEG
                     NSNIIMCERGIRTYNDYTRNTLDLAAVPIIQKETGLPVVVDPSHATGVRYLVKPMSLA
                     SFACGADGIMVEVHPDPENALSDGAQSLCFNEFEDLMKSINNY"
     gene            complement(8573..9079)
                     /gene="folK"
                     /locus_tag="KR250_001908"
     CDS             complement(8573..9079)
                     /gene="folK"
                     /locus_tag="KR250_001908"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HBH0067844.1"
                     /translation="MNKAYLGIGTNMGDRFDNLSRACELLKNSDSIYKVKESSLYETK
                     PWGYTEQADFLNMCVEIETEFEPYELLEYCQEIERELHRERIVHWGPRTIDVDVLFFN
                     DVVSTDERLTIPHPRIQDRAFVLIPLMDLNEELIINEKTIKEHLNLLSAEEREEVKEL
                     VGYERKPI"
     gene            complement(9072..9437)
                     /gene="folB"
                     /locus_tag="KR250_001909"
     CDS             complement(9072..9437)
                     /gene="folB"
                     /locus_tag="KR250_001909"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HBH0067845.1"
                     /translation="MDKILLSNLGFYGYHGVLKEENFLGQKFFVDMELYIDSREAGLS
                     DDINKSVSYAEVYNVVKDITENKQFNLLEALAENIAEEVLNKFILINGVMVRVRKPEA
                     PVNGIYDYFGVEIRRTRDE"
     gene            complement(9448..10239)
                     /gene="folP"
                     /locus_tag="KR250_001910"
     CDS             complement(9448..10239)
                     /gene="folP"
                     /locus_tag="KR250_001910"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HBH0067846.1"
                     /translation="MFDYGKRTYIMGILNVTPDSFSDGGDFNNLDIAIQHAKDMVDQG
                     ADIIDLGGESTRPGHSYVDSDEELRRVIPVIKKLKQELDIPISIDTYKADVAEEALKL
                     GVTMVNDVWGLRKDKNMASVIGKYDAEVCIMHNQDGTNYDKDIMESIKDFFKVSIEMA
                     MSCGVKKEKIVLDPGVGFGKDFEQNIEVLRRLNELKDLGYPILLGTSRKSVIGKVLPV
                     EPKKRLEGTIATTVLGIRDGVDIVRVHDVYENLMAARMTDAIYRK"
     gene            complement(10303..10875)
                     /gene="folE"
                     /locus_tag="KR250_001911"
     CDS             complement(10303..10875)
                     /gene="folE"
                     /locus_tag="KR250_001911"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HBH0067847.1"
                     /translation="MNKVDKEKIQHAVREILEAIGEDPDREGLIETPNRVARMYEEIF
                     SGLSEEPRDHLKVLFADEKHEELVLVKDIPFYSCCEHHLVPFFGKAHIAYLPKGGRLT
                     GLSKLARVIDTLAKRPQLQERITKNAADIIMEELQPYGVLVVVEAEHMCMTMRGVKKP
                     GSKTVTSAVRGIFEKDIASRAEAMSLITMK"
     gene            complement(10901..11602)
                     /locus_tag="KR250_001912"
     CDS             complement(10901..11602)
                     /locus_tag="KR250_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0067848.1"
                     /translation="MIGCIYEVYNEEMTKIIENQNTLAVYLVGSSKDVDFTLYDVVIN
                     DIDVFVFVKEGEKQERILFKKKGIEFDVNYFSKDGVKKLLSNREYFFVKEMKDAKVLF
                     DRENISHIIKDISRDIYLEGPSKMSLEEKSFIKQDIGAKILNLKNKEKFDVFEYHFLT
                     NLYLKDIIIGYFNINDKWVPKDKKLLKVLKKENNELFELVSKVSENYDYQRLLDVYNY
                     IFKEIETKEVIKLIF"
     gene            complement(11636..12379)
                     /locus_tag="KR250_001913"
     CDS             complement(11636..12379)
                     /locus_tag="KR250_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HBH0067849.1"
                     /translation="MKNNVGFDSSLSKFGIGLFETIRVKNGIAVDLDIHIERMLSSIN
                     CLDLDINYKKDFLINEIVTYIKKENVINKALRITVFDEGYNISIRDIPYNQETYEKGF
                     KLAISPIVRGNSLIHRHKTTNYFENIYTKNIANKTGYNDGIFLNSEGVILECSMSNIF
                     FIKGERVYTPSDRLPILNGIIKKRIIEICDELHIELIENEINISEISSFDFVFVTNSL
                     MGAIKVTEIDKIKFNKENVVFDKIVKLLE"
     gene            complement(12369..13718)
                     /gene="pabB"
                     /locus_tag="KR250_001914"
     CDS             complement(12369..13718)
                     /gene="pabB"
                     /locus_tag="KR250_001914"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HBH0067850.1"
                     /translation="MIREINTKLNSFEIFTIFRNEHDSFILDSAMDKEKLGRYSFISS
                     QPFKVLKYKDTDENPLEVLKEELHKYRVVNDTNLPFVGGAVGYLSYDLGNYIENLPRT
                     AVDDIEMPDMYFGFYNHVIVIDHLVQKTYIATPNIDIELEEKIIDDIEQRILKEEKKG
                     IDSICYEEKEVTPIRLKSNFTKEEFKNAVQSVREYIRQGDIYQANLTQRFSGETELTS
                     FELYRDLRRFSPAPFGAFLNFEDAHILSNSPERFIRCVNKRIETRPIKGTRPRGKDKE
                     EDLRLQQELRNSEKDRAELLMIVDLERNDIGRISKTGSVKVPELFVIEPYANVNHLVS
                     TVVGELKDDKDATDVIKATFPGGSITGAPKIRAMEIIDELEPTQRNVYTGSIGYIGFN
                     GDMDFNIAIRTIIKNDKKVYFQVGGGMTWDSDPDEEYQETLDKAKSIMKALRGYYEE"
     gene            complement(13720..14304)
                     /locus_tag="KR250_001915"
     CDS             complement(13720..14304)
                     /locus_tag="KR250_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HBH0067851.1"
                     /translation="MILMIDNYDSFVYNLVQYIEELGEAVVVKRNNEIKISDIEELNP
                     EVIVLSPGPCSPKEAGICIDIVEHFKGKKPILGICLGHQTIGHVFGGDIIKAQQPVHG
                     KVYSINHTNKGVFRGLKNPLNVTRYHSLIVDSNTVPKELEITAITDKGEIMGIRHKKY
                     LIEGVQFHPEAILSEYGHEMLKNFITEARERVHM"
     gene            complement(14328..15047)
                     /locus_tag="KR250_001916"
     CDS             complement(14328..15047)
                     /locus_tag="KR250_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBH0067852.1"
                     /translation="MTSWKKKTVYKCLIAVALFCGIVLISNFSKVSALMMDTNGNVLI
                     KHGSREKKLIAITFDDGPHPKETSQVLDVLKKYNVKATFFIAGKHAKWYKEPLVRASK
                     EGHEIGNHTFNHPDISNLSSSQIEEEIVKCEDILKEVTGKKPTLFRPPFGSYREKDLI
                     EIAKKHDYKVVLWTGVDVKDWKNPGANSIADKIINKVQNGDIILLHDYATNDTVEALD
                     MFIPKMIEKGFKFVTVSELIK"
     gene            complement(15075..16139)
                     /locus_tag="KR250_001917"
     CDS             complement(15075..16139)
                     /locus_tag="KR250_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HBH0067853.1"
                     /translation="MKRLIALVLVVLIVLFYSPELLKEGYDLKEKFFQSEIWNEFLDK
                     ININDNSKKDKKEKSQNLQSNNTEGENLGESDIKNFEKISLGDNLSYVLSSIGKPGRI
                     DISEYGFDWYVYNQYGKEFAMVGLENDEVVALYSNSINSCENQDIKLNQDRQTVRTKI
                     TPLKYKRKGNTRYIINSENQYDIISKEGKYITIFYDIHEENRVCSYLIIDKSTEDEFK
                     NLYPDDSEELKKCFELEVIDLVNSVRNQRGLNSLRYSEQATLSSRKHSEDMRDNNFFD
                     HVNKKNETPFDRMKREGIVYTSAGENIAAGQINAIYAHEAWMNSEGHRKNILGNYNNI
                     GVGVIFGGSYKTYYTQNFYK"
     gene            complement(16283..17092)
                     /locus_tag="KR250_001918"
     CDS             complement(16283..17092)
                     /locus_tag="KR250_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0067854.1"
                     /translation="MNLSKEYVNLYDNYLKYINEVNKEIRSIKNMKKRVLKRGDFAPE
                     NEVLELEGKTIRDIDDVDTKKPKNKIYKFSNGDVYVGKFLEGKMEGKGSYTFFVDEGT
                     VMEYIGEFKEDKKNGKGNFIFSNGNEYIGYFEEDMMSGIGNMLYSSKDEYIGTWKNGK
                     KDGKGIYKWSDGCIYAGEFKGGKMEGYGICYNSKGEVLYEGEWKNNLIHGKGTYIWEK
                     GKKYIGEFMHGKKHGQGTFYLNNELVYEGTWKFDKPSIFDRTLDEIFSLRL"
     gene            complement(17334..18200)
                     /locus_tag="KR250_001919"
     CDS             complement(17334..18200)
                     /locus_tag="KR250_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBH0067855.1"
                     /translation="MSEVKNTLQKHPVLRYSTMLFGMLMTSIGINGFLRPAHLLSGGA
                     TGIATSINYLTNINVGLLTFLINIPIFILGFIYLEKEFCISSLVNMIVFSLLLGATQE
                     ISNIIPIHDILLQSVYGGILSGLGVGVVFRTRSSQGGTDIIAAILKIKKNIEMKDTAL
                     AINGLIVLTGSFLFGLDLALYTLIGLFLNAYSMSFIKDAMNYQKSVMVMSNEVDLIAE
                     DIMKSLVRGVTFLDAEGAYTHQKKKIIYTIVSSNEIPKIKDIALKYDKKAFISVNDVT
                     EVKGRGFKAKDL"
     gene            complement(18720..19409)
                     /locus_tag="KR250_001920"
     CDS             complement(18720..19409)
                     /locus_tag="KR250_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HBH0067856.1"
                     /translation="MYNVLQTPVFGIIVTIVFFNLGRYIQRKTSNPICNPLLIAIVGI
                     ILFLSISKIPYENYKIGADSLNFFLGPVTVVLAVPLYKQFELFKKHMFEIIVGIGCGI
                     VISFVSVLIIGKIANSDVSIINSLIPKSITTPMGISLANSLNGVESITVVAIIFTGIF
                     GGMVASTVFKLGNINHPVAKGIALGTSAHALGTTKAFELGEIEGAMSGVSIGVSGTIT
                     VILIPIIMNFI"
     gene            complement(19402..19773)
                     /locus_tag="KR250_001921"
     CDS             complement(19402..19773)
                     /locus_tag="KR250_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HBH0067857.1"
                     /translation="MKVFNQLVIILSIWVIGEYISSFIQGIILIPGSIVGMLLLFLLL
                     QFKVLDLSSIENVSGFFLDNMAIFFIPAGVSLIKSLDLIRENVLVLLLVICLSTLIVM
                     YTTGIIVEKMIKKKEEGQKNV"
     gene            complement(19955..21301)
                     /locus_tag="KR250_001922"
     CDS             complement(19955..21301)
                     /locus_tag="KR250_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBH0067858.1"
                     /translation="MPRKILDISLIEDNKSDLTFFNIEEEDGDETLKALKQSYVTTNY
                     KEETLLNLQGALNSKSDTTSKELKLESYKRYNNALDLAYKNYITSAINMVNKALEINP
                     KDIDILNLRGLLKLLKCDFAKSFESFYTALCYENNHISRKYVNLISSEDFKTFLGRYN
                     HSIRFINEELNYESIHILENIIEEEPELIEPYVILSLLYDKLGNVKKREGYLDKLKEL
                     DKDNPVFEKNEEEKEDTSKKEEIKKVKRKKSILPYIIVGCVLIGMGIYLIQSKKRIEN
                     LNNQISSKEEKLSETDKKLGETSKKLEKTNKELNEVKNKEPEREITVADEEDLYYQAL
                     NLKKNKEYEKAIDNFKSVVSSGKTKKYISESIYQLAITNKLLGNKDEAIKYYKKYINT
                     YTKNDQYYDDSYYELGMFYYDNGDLKNAQQTFYSLRSEVPDSMYNNSKIKEILSEK"
     gene            complement(21483..22076)
                     /locus_tag="KR250_001923"
     CDS             complement(21483..22076)
                     /locus_tag="KR250_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HBH0067859.1"
                     /translation="MNLNNITNYVQSICENISNVLDVDVTLVTKDLTRIAGTGIFRGK
                     IGETISEKSVYSLVLKDGKSYVINKEIEENCNKCANRENCKELADICTPVKMGEHILG
                     ILGIAAFNEEQKEKILSKDKELTAFVRSMSDLISFKLDELYKQEVKTIDDLEREAIEN
                     AIKKYGSNTEGMKKVAEALNIGIATLYRKVKKFNIKS"
     gene            complement(22141..23610)
                     /locus_tag="KR250_001924"
     CDS             complement(22141..23610)
                     /locus_tag="KR250_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HBH0067860.1"
                     /translation="MSNVNKKLVIAIGAVALVVIIFIGIMCMQFKGEKIAKNTYVNGV
                     NIGKLTKSQAKQELAKKYKLENVDFNYNDKSWKVKSKDLNLSTDLDKTVENAYNLNRK
                     SAFFGNLSKTISANFGKKSNLVVAINYDKNKLKAEMEKIAKEIDVDVKDATLDISGEK
                     VKVIPDSDGLKMDISKSMENFDNQTKKGNYKNELVVKATPAKVKKEQLANIDTNLGTY
                     STTFKTSQINRSINIKLATDNISNVLLMPGETFSFNKHTGKRSKENGYKSAPVIMEGE
                     MEEDYGGGVCQVSSTLYNSVLYAGLEIVNVKNHTIPSSYVPKGRDATVADSGIDFLFK
                     NNLKHPVYIKNYVSGNQIVCNIYGSAEDKQNITISTKLDGVSQTTMKRVNDPTMPKGK
                     EKVDKSGRNAYSVSTYRTFNDANGKKIKTEKIANSYYPKKEGIILVGTMEPKPEEKPN
                     TDENKNNQNTNNQNPNNQQKPETPPTDNKPNETQPQPQA"
     gene            complement(23725..25116)
                     /locus_tag="KR250_001925"
     CDS             complement(23725..25116)
                     /locus_tag="KR250_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HBH0067861.1"
                     /translation="MNFRDLFEKAVDFIDEKRKSIVAISLSTLGVIALIIVFFLSSNE
                     FSVGNEANELLKIIEKRQYSIAVDYYTSIEKKFSDSKMERFNKSVSKKINKLLLNSGD
                     KYLDGDISQESFIGLINTVKELNKISIDVDDLLAQADRVREMYKEENTTYDIAINYIS
                     TVSILNGMGSNLDVYKQNIETIKESRDVYDSAVKDQKAYKYYEAIEKYNKVLKEDEKY
                     YSMAQNGKEQCIEEMYDYYISRAEEANTSGDYERALQYIEYLKDDYSDDEKVQSLEKK
                     YKKNLSLYTLTSDDIINVIAKKSGKDKANLSINSLPQMIKNNKYYYAEVYEYDKLINE
                     VLVSAKTKDLYSYKDGKKDYKVDYGDGYFRILEDGSYQFGITKDKAKFVLTNTLDEKE
                     NKYKKIEILDIEKADRYVKSKKSLEELFGKYKNIYYYAVVNKGLFRGKEVYAINIYNE
                     KIYAISENGLNEY"
     gene            complement(25282..25530)
                     /locus_tag="KR250_001926"
     CDS             complement(25282..25530)
                     /locus_tag="KR250_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0067862.1"
                     /translation="MMICFAMTFALVLINSVMGVHIGLDYYFLMGSVLTVCLTIGIVL
                     CNARKIFDSKAIHKTRTKTVSNNKKQKSHSRDIKRKIS"
     gene            25796..27925
                     /locus_tag="KR250_001927"
     CDS             25796..27925
                     /locus_tag="KR250_001927"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HBH0067863.1"
                     /translation="MPNLPSLGSKAPDFKANTTNGPIRLSDYKGNWIVLFSHPGDFTP
                     VCTTEFLCFAKYYDEFKKRNTELIGLSVDSNSSHLAWMYNISLLTGVEIPFPIIEDRD
                     MRIAKLYGMISKPMSDTSTVRSVFIIDNNQILRTILYYPLTTGRNIPEILRIVDALQT
                     SDRDNIVTPANWFPGMPVILPYPKNYKELKNRVNSCNKKYSCMDWYLCFVPDNYNDEE
                     VSKKIDNTCSWKKEHTKNIENECNCEHEHHDYLNKALDCKQEHKTDIKDDCNHEKKHT
                     KNTNKVHNSKQDKFKDKSCDEMNFNYDKDESCDKINSSYNKEDSSYEDFYKHNYKNYD
                     YTSEKNTKKIAMKTLKDSKKLVRPQITDPYNPIVENANCPDINPIVAEYVLGNPTNVD
                     AQLLDAVIFAFAEIDQSGNLFIPYPRFLNQLLALKGEKPSLKVIVAIGGWGAEGFSDA
                     ALTPTSRYNFARQVNQMINEYALDGIDIDWEYPGSSASGITSRPQDRENFTLLLTAIR
                     DVIGDDKWLSVAGTGDRGYINSSAEIDKIAPIIDYFNLMSYDFTAGETGPNGRKHQAN
                     LFDSDLSLPGYSVDAMVRNLENAGMPSEKILLGIPFYGRLGATITRTYDELRRDYINK
                     NGYEYRFDNTAQVPYLVKDGDFAMSYDDALSIFLKTQYVLRNCLGGVFSWTSTYDQAN
                     ILARTMSIGINDPEVLKEELEGIYGQF"
     gene            complement(28182..28781)
                     /locus_tag="KR250_001928"
     CDS             complement(28182..28781)
                     /locus_tag="KR250_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H family protein"
                     /protein_id="HBH0067864.1"
                     /translation="MAKQKFYAVKKGKNIGVYNTWDECKKQVNGFSGAEYKSFSTFQE
                     AKEYIDGSEKLSFQEDKEFIEAYVDGSYEHSVKMYGSGVVILKNNEVIKTYSEKGKEK
                     TLVSMRNVAGEIEASKIAMQYCIDNNVQNLILYFDYEGIEKWCTGVWKTNKEGTIAYK
                     NFYDSIKNKLNVKFTKVKAHSGNKYNEEADKLAKKAIGV"
     gene            complement(28799..31918)
                     /locus_tag="KR250_001929"
     CDS             complement(28799..31918)
                     /locus_tag="KR250_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HBH0067865.1"
                     /translation="MDNIISVLNDVVFLDIEVSGLDCLNSEILEVGAVKVKDWKIYTY
                     ESLIKNKFEVPVEVFSVCKNLDKNDLEIANEIELVEDRLVNFVEDSFIICHDLSLKKK
                     FLEYHMPKLKNKFIDLIELAVILEPYHKDYSLEYLKNTLTNCNSKVENRALSDAIDII
                     NIVNCLLVKFNNYEKTTLEPLSFKINSYLKKFNLPTWEWSKFLEEANYDLSNNINIKK
                     EYNIFDSKEEKKKERETLKILNEEEKNYEELLKYKTIWENKEGFTYEYRPGQYELTKT
                     IRELFRNSEDEEKIACIEAPTGIGKSVGYLLPAILEARINKKRLLISTDTKELQIQLI
                     NKDIPNVLDSLGLNGKVSYGYIKGKNNYICIDRLEAYIDDYESQNPTKGEILSLIFLK
                     RLVEGGKYGDIEEINYLVFDNFKEISTHLRNVSCDPNMCRPKKCKKDCLYKNRIEELK
                     EEHITVVNHSLLAKWPYKDEKPLENIIVDEAHNLTEKGYDFFSSIINSKSLRYLLQEI
                     YPYEFIQNSSFIYKKYSRNMRKIKAFDKFYNVLKIGREDKQKIARSINLIIEEIDSIL
                     KFGNCNEYNNVSNYNLRWELNLQIDEIVGKLKKDGIDTEISYRAYSEKIKLSCEKIIK
                     NLVSIIIIIYRNIDDDSIDKEADIYKFGKAKTRDLEDIKIIFEIFLEYDEKDDYARIV
                     EIDKNYNDFEFRVVPLKIADLFEENILSQLEKGIFLSATLSLSESMSYFKNTLGIDRV
                     KNVEKIIEPIFDYKNRVSVVGFSDICEYRNSEFPNEMSKIISNISKITEGHTLALFNS
                     KDRQEKTYEILKKYLHSFNLEIYADKKGIRHLNDLNRKCVVLGSKGCFEGVDIPGDGL
                     VCVTLDKLPNLNPKDPLYFTIMKKYGIDYYTINYPQMTIKVKQAMGRILRSKYDYGCF
                     VIFDVGTNISVLKRLEKDLHDCKISKVNSNEFYTYIRRHLNKYRSLILKSVIFDTIKA
                     LNVDAKMDNNDVDKDIIKKDINENIRQRAVKGEVYHIDIIKKDMKVKYFDRNYLINLD
                     IFMREEDKN"
     gene            32139..32963
                     /gene="pdaA"
                     /locus_tag="KR250_001930"
     CDS             32139..32963
                     /gene="pdaA"
                     /locus_tag="KR250_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-lactam-biosynthetic de-N-acetylase"
                     /protein_id="HBH0067866.1"
                     /translation="MKKDSLKKYIMIGAFALILFGIASINFKSLDKTKTQISSPTLDT
                     HEYDWYFNPREDGKQPSPIKEADFFKKYGAYYVGNPNEKVIYLSFDAGYESGNTPKLL
                     DTLKKHNAKAQFFVVESYIKSNPELIKRMEKEGHLVCNHSKSHPSMAGITDFEKFKEE
                     ITSVEKAYKDVTGKEMPKYFRPPMGKFSEQSLKYTQDLGYKSIFWSFAYVDWYEKKQP
                     THEFAKNKIYSRTHPGAIVLLHPNSSTNTEILDEVLTHWEKEGYKLKTLDYLNNKK"
     gene            complement(33060..33845)
                     /locus_tag="KR250_001931"
     CDS             complement(33060..33845)
                     /locus_tag="KR250_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBH0067867.1"
                     /translation="MIVKIDNTLEKEFWQYVSHEESLNLFIIGYVENYGFSSQYQDIW
                     SQVEDGNITSIILKNKSTLIIYSFKNNFNIGEMKNHIKDLDVESISGKKCVIDRLISK
                     YKDFYEKLDNKFCVLKEIKEIDFSNMKEYKIENAQEKDIDEIGKLLNRSDYKVSKNYI
                     EERKVHLKEGNVRAYFIRNDDTMISTVSTGMETSFLAMVVSVSTDKRYRGKGLASYMV
                     YNLSKELLLEGKVPCLFYNNDVAGKIYHNIGYKEINEWTILFK"
     gene            34310..34675
                     /locus_tag="KR250_001932"
     CDS             34310..34675
                     /locus_tag="KR250_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0067868.1"
                     /translation="MKIRAEYTCLLEIVHDIIKGKWKTILIFELRQSNKTFSELQHNI
                     YGINQKMLIQQLKELRDFGIIDKISSAGYPLHVEYFLTNRGKKMLKAVEIMQEIGIEY
                     MLEHGQQEILDNKGICYNK"
     gene            34753..35307
                     /locus_tag="KR250_001933"
     CDS             34753..35307
                     /locus_tag="KR250_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBH0067869.1"
                     /translation="MSIALILIDIQNDYFKNGKCELFQSEETAENAKKILLFFRKNNL
                     PVIHIQHISTDKTASFFLPNTHGSEINKIVFPLDNEEIITKHTPDSFFETDLQSILEK
                     KNITNLVICGMMSHMCIDTSVRTAKKLRYDITLISDACTTKNLSWNNIEINANTVHQV
                     FMASLQNSFADVKNTDKFLSNYHK"
     gene            35527..36201
                     /locus_tag="KR250_001934"
     CDS             35527..36201
                     /locus_tag="KR250_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HBH0067870.1"
                     /translation="MNHHILIQFEYIARIITAGFCGALIGYERKNRLKEAGIRTHFIV
                     ALASALIIIVSKYGFNDIVGTPGIGLDPARVAAQVVSGIGFLGAGLIFIRNQSVSGLT
                     TAAGIWATAGTGLAIGSGLYLVGIVSSIIIVIVQITLHRDRKWMKLPTAEQVSLKISN
                     STDSIEYIEGKFKKSNIEIINMNIKRLDGDWLEVVIYTKLPKGYKKTNLLKLFSDNPL
                     IETLEI"
     regulatory      36556..36652
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            36706..36882
                     /locus_tag="KR250_001935"
     CDS             36706..36882
                     /locus_tag="KR250_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0067871.1"
                     /translation="MFRDEMDKCTHMLTAYISSLYDYCDFIDTQLDDFILEYGENVVE
                     SCLHQVMVLVSKYN"
     gene            complement(37070..37309)
                     /locus_tag="KR250_001936"
     CDS             complement(37070..37309)
                     /locus_tag="KR250_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0067872.1"
                     /translation="MKCSNCGSVNIEKGILTTGGIYAETIGLKYRGDGKLDKVINKTF
                     PVQSTIYSDLCLDCGEIVRTYIKDNTDRNWCKDKK"
     gene            complement(37443..38252)
                     /locus_tag="KR250_001937"
     CDS             complement(37443..38252)
                     /locus_tag="KR250_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBH0067873.1"
                     /translation="MKDYYKIGEISKIYGIGRDSLMYYEEIGILRPVRDINGYRMYNI
                     SDIWKLNLIKEFRSLNFPMKKIKEYLDDRSIESTKNILNEELDLIDKKIAEFISHKEN
                     IIKRLSSIESVIQNTKIDEIEVVYIEKRKALELNADIKRDEDFDFLIQKLQKEYEDRF
                     NILGNNNIGSAFSTEAINKGIFNEFKSVFCFLESNEKVYNIVFDEGYYVTLNYTGSNS
                     NNKIYMEKVFKFIEENNYKIIADPIEIYKIDIHETGIVEEFVTEIQVPITK"
BASE COUNT        12364 a         6525 c         3934 g        15475 t
ORIGIN      
        1 tataacttcc attggatcca taaagtcaag gattaagata gatacaaata gaattgaaga
       61 aagtagagtt gaaaacatta aatatttaaa taaaattggt tatttgtttt taatattgat
      121 gattataatg acaacacagt tttttactac tcttttatca ataaaaacaa aactatcaac
      181 tgctatgaat attacaacgc ttttagttat aatttattta atagcaacat atattaattc
      241 cccaaattta aaattcaact catcttattc tccagaagaa gatgagaagt attggatagc
      301 aggcatcatg tataataatc caaatgaccc atcattaatg gttaataaaa ggtttggtat
      361 tggatggact ataaattttg gcaatcctct tggaaaaata ctatatattg ctatagcttt
      421 acttttaatt ttttcattgt ttagcctaat aaaatctctc ttactttaaa ataccataaa
      481 aggtatctta cttgtattat tgggtattat attttgtagg ataagaaata tttcatatta
      541 tcgttgaagt atagtattta tatattacta agaatggaga ttttcataat gttgtcaaaa
      601 gctgaaatca aatattttaa tgaatgtgct tctgagatac tttcttctga aaaagttcaa
      661 cttatgagaa catttcctca tcatggcaat gtttcatgtc ttgaacatag tttgtcagta
      721 gcatattata gttatctatt atgtaaaaaa ttgcacttaa gtgttgatat tcaaagtgtt
      781 attagaggag ctttactaca cgactttttt ctttatgatt ggcattacaa aggtgataga
      841 aaaggattac atggatttac tcaccctaga gaagcattaa aaaatgcaac tttatttttt
      901 caaataaatg aaaaagaaac ggacataatt ttaaaacaca tgtggccact tactgttaag
      961 ccacctagat acaaagaagc atttattgta tgtttgttgg ataaattttg ctgtttagtt
     1021 gagactttaa agatacattc tttattatct ccttaccatg tataggacaa ttcccacaac
     1081 acttttataa cttaattttg aagaggtatt aagtattata ttcaacttga tacctcttta
     1141 ctatttatta ttttatataa taatattttc tttataatat agttataaat aacaacttct
     1201 accctcgata acttcccctt cttatagaca tgcttactat cttaaataac aagaaaccag
     1261 ctattaaatt tattagtatt gtttatacta ctctaacaat aatatcataa ataaacctcc
     1321 aaatagcttc tctttcacta ccttttatct taaactatat ttttttctta aaatttgtaa
     1381 aaaacattgc ataatcttta taaatgttct aaaatttaat ttaagcatca aaattagaaa
     1441 ggatgatatt atgtttaaag aaatgagatt gaaaaaaaga gaaatgacaa aagaagatac
     1501 tgttgaagta ttaaaaaatg gtgaatttgg tactttttcc actatatctg aaaatggtta
     1561 tccttatgga gttgctgtaa attatgtata ctttaatgat tctatttact ttcattgtgc
     1621 aagaaatggg cataagttag ataatatatc gaaaaacaac aaagtttctt ttttagtagt
     1681 tgctaatgaa agtgttattc cagataaatt tagtactact tattctagtg ctatagtttt
     1741 tggaaaagct tgcactgtag aaaatgaaga aaagaaaaat gctcttgtag aaataataaa
     1801 aaaatattct aaaggattct ttgaagaagg catgaagtac atagagaagg atatgaactt
     1861 aacaactgtt gttaaaatag aaattgacca tatttctggt aaagcttcac gtttataatt
     1921 aagtaaaaat acctaaaagt atatacagtc gcttttaggt atttttatat gatattctta
     1981 tatgttaagt ctttttaggt aatctcttat acctatcttg gcatcatcat ttggtcttat
     2041 atcagaatta tctcctgcta tatgaagtat tccatacgct ggataacctc ctcttactga
     2101 acatggaact gacaaaagag tcattcccat atgttgggca aatatattca tagcttgcaa
     2161 agctaattct cctcctccat gttgtgaacc tgctgtagca aaacaaccaa atattttacc
     2221 actcaaagga gtacctaccc aaatatcaga aaaactatcc ataaacatct tcattggtcc
     2281 agatacattt ccataatatg ttggtgaacc cataaatata gcatcataat ctaatatttc
     2341 ttttccagat ttaattacat ttatactttc aaattcgtct ttgtattctt tggaatttat
     2401 caaataatat tgaggaagtg tttttgcaac ttcgtcactt actctaaata tatctacctc
     2461 gatattcatt tcttcaagcg cttctttata ttctctagct aataaatatg tactcccaca
     2521 aacactatga aaaattactg ctactttcat cattatcccc ctaattaaac tcaaatttat
     2581 tttttatata gtatttttat tatcaaacac tgctaaatag tactaaatgg attcaaatat
     2641 acattacttt ttattacttc aatttcacta aattcatcta aaaatctctt cataacattt
     2701 ttgaatatat cttctgtatc aaaatgacca caatccacta tacacattcc aatatcaagt
     2761 gcatcttgtg cctcatgata ctttacatca cccgtaatta gtacttctgc accttgtctc
     2821 ttagcttttt ttacaaattc tgaaccagct cctgttacaa cggctacttt cgttatgtct
     2881 gtactaaggt ttccaactac tcttatgtgt ttcatattta atttctcttt tattttcctt
     2941 gaaagacttt ctaatgtcat acttttatca agtttactta ttctacctag tccaaatatc
     3001 tcacctttat tttctaactt gtacaaatca tatgcaactt cttcataagg atgtgcatct
     3061 aacatagaac ttattacccc acctaaaagc ttctgtggta ctacagtttc aattttaact
     3121 tcatttacag tttctatatc atttacactt ccaagaaacg gattagagcc ttctaatggc
     3181 ttaaattgtc cttcaccttc tattgaaaac gtacaatcac tataattccc tatatgacct
     3241 gctcctgaat tacttaaaac ctttctaagt tcatctgaat agttatgtgg aacgtatact
     3301 gctaatttat acaaattttc gcttttagtt atatctaaaa cctttgaatt accaaattcc
     3361 ataatttcca taaaatagtc atttagacca tcaaatgcta tatcaaaatt cgtatgcata
     3421 gaataaagtg atatatcatt tttaataagt ttttggataa gtctgccttt taaatctcca
     3481 ctattaattt tattaatttt cccaaatata aaaggatgat gtgtcacaat taaatctata
     3541 tttttggata tagcttcatc aataacatct tcattagctt ctaaagatac caataccttt
     3601 tttacatcca tatcaaaatc tccaactata agacctacat tatcccaatc ctctgctaag
     3661 tttagaggat actttttttc aatttttcta gttagactct ttaatagcat atatacctcc
     3721 tatttatttt attaaatttt caagtttttt tattgcaact tcgctttcct ttctcttctt
     3781 atctatagct tccccatttt tcccttctaa tttattaact atagaactat atgtctttat
     3841 ctttttttct ataaaatcat taaaaagaga atctttgttt tctaaaagct ttattccaac
     3901 ctcataatat atctcatctt ctattatagt atttttacct gtatatttgg caacaattat
     3961 ctcatatatt ctaaaatctt ctctcactaa aacttcttct aaaatttcat atccgttgtt
     4021 taaaaggtaa tatctaagtt cttcttgtgc ttgcattggc tgtaatatta atttttctac
     4081 attatgtgct acttcttttt tagcctcgag aagctcactt atcaagattc cacccatacc
     4141 agcaattatc acttcttcta cttcccctat ttcaagtatc tctataccag aacccaatcg
     4201 taaatcaact ttatctaaaa gattgttctg tatgacttct ttatgtgcat tgtcaagagg
     4261 tcctttatta acatccgcta gcactgcaaa tggtactttt ccctctttta acaaatatac
     4321 tggtatgtat ccgtggtccg tccctatatc agctattttt ttaccatctg acactaatga
     4381 tgctattttc aataatctat ctgttaattt caaatataat tctcctttca attcccttta
     4441 tttataggat ttaatccaag ttatagatat tagactgaaa taactacaaa aataaaatta
     4501 aatctttact acaaaatagc catgagatat cacttatctc atggcattta tcacaaaaac
     4561 atcaatgatg tatcttttta gtctaaataa tctctaagtt tttttgatct acttggatgt
     4621 cttagttttc ttagtgcttt tgcttctatc tgtcttattc tctctctagt tacatcaaac
     4681 tcttttccaa cttcctcaag agttctggct ctaccatcct caagaccaaa tctaagcttt
     4741 aatacttttt gttctctatc atttaatgaa ccaagtacat cttctatttg ttcttttaat
     4801 aaagaatatg ctgctgcctc tgctggggct ggagcatcat catctggaat aaagtcacct
     4861 aaatggctat cttcttcttc tcctattggt gtttctaaag acacagggtc ttgagctatt
     4921 ttcattattt ctcttacttt atcttctgtc atttccattt cttttgcaat ttcttctggt
     4981 tttggatctc ttccaagttc ttgaagtagt tgtcttgata ctcttattag cttatttata
     5041 gtctctacca tatgaactgg tattcttata gttctagctt ggtctgcaat agcacgtgtt
     5101 atagcttgtc ttatccacca tgttgcataa gtactaaact tatatccttt tgtatagtca
     5161 aatttttcga ctgcttttat aagacctaaa tttccctctt gtattaaatc caagaaaagc
     5221 atacctcttc ctacatatct ttttgctata cttacaacta gtcttaagtt tgcttcaact
     5281 aacctttgtt ttgctatctc atcaccttcg tgcatccttc tagcaaattc tacttcttca
     5341 tgtggtttaa gtagaggaat ttttcctatt tcttttaagt acattcttac agggtcatct
     5401 atacttatcc cttttggtaa agataaatcc acagtttcta tttctattgc agaagagtca
     5461 tcatgtgaaa ttgcctctgc atcttcatct aagtggccta tacttaaatc gtcatcagca
     5521 accgaaaaat ctatatcagc tttatagttt tttgtttctg ttatttctat tcccaaattt
     5581 cctaaagtct cataaagatt ttctacttga tccttatcaa gttcagtctc cgaaaaagct
     5641 tccattatct ctgcaagtgt cagagaacct tgcttttttc ctttttctat taatgtctta
     5701 gcagtaacct tttttaactc ttttttattc gatttatttt ccacactcaa acctcctttc
     5761 tctacaagct ctttaaaatt ttatttatct caactatttt taaagcaatt tccataactc
     5821 taccatctac ctctttcata tcattattat tttctaattc ttgttgttct ctcaacaagc
     5881 tattaatttg ctcttctaat gagttttttc tgatatttgt tattattcct tctatttctt
     5941 ttgtgttctc taagtttata ctattcaaag aaattgaatt taattctttt aagtattctt
     6001 cggatatgtt taaacttttc aatttgtcaa tagttatttt gtccaattct tgatttttaa
     6061 tcatgtaatt taaaatttct ttactttctt tcaataaaaa atcactttct tctactttaa
     6121 gtaacgctat ttctctaatt tttttgtctt ctaacattat ttttattaga gtttcttcaa
     6181 ctaattgttt tccgtcttgt ctgacttcga ctttttcaat tactttttct tctattttct
     6241 tttgattttt gttattatat ggcttattgt aatttttacc ataaacttct ctctttatag
     6301 actcgacatt aatatctatt tgattgctta aatactttgt ataaaaatca atttcaacag
     6361 gacttgtcaa ttgttttata attttagaag cttcttttgc gaacttcact cgttcttcat
     6421 ctttttgaat gttaaattct tttttaagat gatctatttt atattttatg tagtgagtag
     6481 aaacatccat agctttttta taatcactaa gtccgtattt tcttacaaat tcatctgggt
     6541 ctttagcatc ttttaagtct aacactttta cattgatacc taatttagta agtatatcta
     6601 tagctcttaa agtcgctttt attcctgctt catcagaatc ataagatata atagcagtgt
     6661 ccgcatatct ctttattaaa atgccttgtt gttcagtcaa ggctgttcca agggttgcta
     6721 ctacattttt tataccatac tgataaagcg aaattaaatc catatatcct tcaactaaga
     6781 ctatagtttt actttctaaa ttttttctag caaaatttaa tccgtataag ttctgttttt
     6841 tattaaaaat taacgtgtct ggagaattta agtattttgg caaagaatcg tctaatactc
     6901 ttcccccaaa tcctattata tttcctctat aatcgaatat aggaaacata actctatttc
     6961 taaatttatc atagcaatta gttccatctc tattttttgc aattagaccg cactccaata
     7021 agtcttgttt tttatatcct ttactgatta gagtattcat aagtgaattc catgaatcca
     7081 aagaaaatcc taaaccaaat ttcttaataa ttttatcatc tagtcctctt attcttaaat
     7141 attcataccc aagatttttg cttcccaata agtttgaaag ataaaatcta gctgcttccg
     7201 tatgaatatc ttgaaatttc ttagattttt ctattttaat cctagtttct tcattcatgt
     7261 tagtgttaat ttctataccg catttatttg ctaaaatttt tacagcatcc ataaaatcca
     7321 aattttccat tttcatgaca aaatttatta cgtctccacc ttcaccacaa ccaaaacact
     7381 tataaatctg ttttgacgta tttatataaa aagaaggtgt cttttcgcca tgaaatggac
     7441 ataatccttt gtaattagct ccagattgtt ttatattcat atattcagat attatacttg
     7501 ctatatcgca tctagctttt atttcttcaa taatgtcttt tatatcattc atattatcac
     7561 cttctggtgt aattttttgt gttaacattt ctattctaca ctatattcta aatttgtcaa
     7621 tcactcaata tacacaattc gacttttttt attgatatcc ttcttattaa tcaaaaaatt
     7681 tttatttttt atattaattc tatcaatatt aaatctttat ttatattaaa taatctaact
     7741 atcaaattat aacttataaa tttaccttag caacgattac gaataaaaaa gaggctcttg
     7801 cctcttttaa taattattaa ttgatttcat taaatcttca aattcattaa agcaaagaga
     7861 ttgagcacca tctgacaaag cattttctgg gtcaggatgt acttctacca ttattccatc
     7921 tgctccacat gcaaaagatg ccagtgacat aggttttacc aaatatctaa ctccagtggc
     7981 atggcttggg tctaccacaa caggaagacc tgtttccttt tgaataatgg gtactgcagc
     8041 taaatctaat gtatttctag tataatcatt atacgttctt atacctctct cacacattat
     8101 aatattacta tttccttcta ttgctatata ttcagaagca cctatccatt cagtaattgt
     8161 ggaagctatt cctcttttta acataacagg tttattttgt tttcccactt cacttaaaag
     8221 agtaaagttt tgcatatttc ttgacccaat ttgaattata tcacttatac tatacagttc
     8281 gtccatatct cttacatcca taagttctgt aataacagcc ataccaactt cgtctttaac
     8341 cgcttttaat atttcaagac cttctttttt taatccttga aatgaatttg gagatgttct
     8401 tggcttgtat gctccacctc ttaaaatatt tgctccctga ctttttacaa atttagcagt
     8461 ctccaaaagt tgctcataac tctctattgc acatggtcca gctattataa gtttatcttt
     8521 ttttacagtt atctttttgt tatttgtatt tatctctatc tttgaattat tattaaatag
     8581 gttttctttc ataacctaca agctccttta cttcttctct ttcttcagca gataggaggt
     8641 ttaaatgttc ttttattgtt ttttcattta ttataagttc ttcatttaaa tccataagtg
     8701 gtatcaaaac gaaagctcta tcttgaatcc ttggatgagg tattgttagt ctttcatcag
     8761 tggaaactac atcattaaaa aatagtacgt ctacatctat agttcttggt ccccaatgaa
     8821 ctattctctc tctgtgtagt tctctctcta tttcttgaca atattctaac aactcataag
     8881 gttcaaactc tgtttctatt tctacacaca tatttagaaa atctgcttgt tctgtatatc
     8941 cccatggttt tgtttcgtat aagcttgatt cttttacttt atatatagaa tcactatttt
     9001 tcaaaagttc acatgctctt gataaattat caaatctatc acccatgtta gtacctattc
     9061 ccaaataagc cttattcatc tcttgttctc cttatctcaa caccaaaata gtcataaata
     9121 ccatttacag gagcctcagg ctttctaact ctgaccataa ctccatttat aagtataaat
     9181 ttattcaaga cttcttcagc aatattttcc gctaaagcct ccaaaagatt aaattgttta
     9241 ttttcagtaa tatctttaac aacattgtaa acctcggcat agcttacaga tttatttata
     9301 tcgtcactaa gacctgcttc tcttgaatca atataaagtt ccatgtcgac aaagaatttc
     9361 tgacctagaa aattttcttc tttaagaaca ccatgatatc catagaatcc tagattacta
     9421 agtaatattt tatccattat attataccta ctttctatat attgcatcag tcattctagc
     9481 tgccattaaa ttttcataaa catcatgtac tctaactata tctacgccat ctcttatacc
     9541 aagaacagta gtagctattg taccttctaa tcttttttta ggctcaactg gaagtacttt
     9601 tcctattact gattttctgg atgtcccaag caaaatagga taccctaaat cttttaattc
     9661 atttaatctt ctcaatactt caatattttg ttcaaaatcc ttgccaaatc ctacacctgg
     9721 gtcaagtact attttttctt tttttacacc acaactcatt gccatttcta tacttacctt
     9781 aaaaaagtct tttattgact ccattatatc tttgtcatag tttgtcccat cttgattatg
     9841 cattatacac acttctgcat catattttcc tattacagaa gccatatttt tatcttttct
     9901 aagaccccaa acatcattta ccatagtgac acctaatttt aatgcttctt ctgcaacatc
     9961 tgccttataa gtgtctattg atattggtat atccaattcc tgtttaagtt tttttattac
    10021 agggattact cttcttagct cttcgtctga gtctacatag ctatgacctg ggcgtgtaga
    10081 ctctcctcct aggtctatta tatctgcacc ttggtcaacc atatcttttg catgttgtat
    10141 tgctatgtcc aagttattaa aatctcctcc atcagaaaaa ctatctggag ttacatttaa
    10201 tattcccatt atataagttc ttttaccata atcaaacaca attaatgaca ctcccttaaa
    10261 ttaaatttta tctcaataaa tgattatatc tataataaat tactacttca tagtaatcaa
    10321 actcatagcc tcggctctag acgcaatgtc cttttcaaat attcctctca ctgctgatgt
    10381 aacagttttt gaacctggtt tttttacgcc tctcattgtc atgcacatat gttcagcttc
    10441 tactactact aagacaccat aaggttgtaa ttcttccatt attatatctg cagcattttt
    10501 agtgattctt tcttgtaatt gtggtctttt tgccaatgta tctattactc tagcaagttt
    10561 agaaagccct gtaagtcttc ctccttttgg taaataagca atatgagctt ttccaaagaa
    10621 aggaactaga tgatgctcac aacatgaata aaatggaata tcttttacca atactaactc
    10681 ttcatgtttt tcatctgcaa aaagaacttt taaatggtct cttggttctt cactaagacc
    10741 agagaatatc tcttcataca tcctagcaac tctatttgga gtttcgatta gaccttctct
    10801 atctgggtct tccccaattg cctctagtat ttctcttact gcatgttgta tcttttcttt
    10861 atcaacttta ttcatttaaa ctccccccat tgatttgtga ctagaagatt agttttataa
    10921 cctcttttgt ctcaatttct ttaaaaatat aattataaac atctaaaagt ctttgatagt
    10981 cataattttc acttactttt gacactagtt caaaaagctc attattttct ttttttaaaa
    11041 cttttagcaa ttttttgtct tttggtaccc atttatcatt tatattaaag tacccaatta
    11101 ttatatcttt taaatataaa ttcgtcaaaa aatgatattc aaaaacatca aatttttctt
    11161 tattttttaa attcaatatt tttgctccaa tatcttgctt tataaaagac ttttcctcta
    11221 atgacatttt actaggtcct tctaagtaaa tatctctact tatatctttt ataatatgtg
    11281 atatattctc cctatcaaac agtactttag catctttcat ttctttcaca aagaaatatt
    11341 ccctatttga taataatttt tttacaccat cttttgaaaa gtaatttaca tcaaattcta
    11401 ttcccttttt tttaaacagt attctctctt gtttttcccc ttcttttaca aaaacgaaca
    11461 catcaatatc gtttatcact acatcataaa gagtaaaatc tacatcttta ctagaaccca
    11521 ctaaatatac agccaaagtg ttttggtttt ctattatttt agtcatctct tcattgtata
    11581 cttcataaat acaacctatc atcttttgtc tccaacaaat tgttaatatt ttaatttatt
    11641 ctaataactt cactatttta tcaaaaacaa cattttcttt attaaatttt attttgtcaa
    11701 tttctgtaac ctttatagcg cccataagac tatttgtgac aaaaacaaaa tcaaaactac
    11761 taatttctga tatattaatt tcattttcta tcaactctat atgtaattcg tcacaaatct
    11821 ctataatcct tttttttatt attccattta atatcggtaa tctatcacta ggagtgtaca
    11881 ctctttctcc ttttataaag aaaatattgc tcatagaaca ttctaatatt acaccttctg
    11941 aatttaaaaa tataccatca ttataaccag tcttatttgc gatatttttg gtatatatgt
    12001 tttcaaaata attagtagtc ttatgtctat gaattaaact attcccccta actattggag
    12061 atatagctaa tttaaacccc ttctcatacg tctcttgatt atatggaata tccctaatag
    12121 atatattata accttcatca aaaacagtaa ttctaagtgc tttattaatg acattttctt
    12181 tttttatata tgtaacaatt tcatttatca agaaatcttt tttatagttt atatctaaat
    12241 ccaaacaatt tattgagctt agcattcttt ctatatggat atctaaatct actgctattc
    12301 catttttaac tctaatagtt tcaaacaaac caattccaaa tttacttaaa ctactatcaa
    12361 aacctacatt attcttcata atatcccctc aaagccttca ttatggattt agctttatca
    12421 agtgtttctt gatattcttc atctgggtca gaatcccaag tcattcctcc tccaacttga
    12481 aagtagactt ttttgtcatt tttgattatt gttctaattg ctatattaaa atccatatca
    12541 ccattaaaac ctatataacc tattgaacca gtatatacat ttctttgcgt tggctctaat
    12601 tcgtctataa tttccattgc tctaatctta ggtgctcctg ttatagaccc ccctggaaaa
    12661 gttgctttta ttacatcagt agcatcttta tcatctttta actcacctac aactgtggaa
    12721 actaagtgat ttacatttgc atatggttca attacaaata gctctggaac tttaacactt
    12781 ccagttttag atattctccc aatatcattt ctttctaaat ctactatcat aagtagttca
    12841 gctctatctt tttcactatt tctaagttct tgttgaagtc ttaaatcttc ttctttatct
    12901 ttccctcttg gacgagtacc ttttattggt ctagtttcta ttctcttatt aacacatctt
    12961 ataaatcttt ctggagaatt tgataatata tgtgcatctt caaagtttaa gaatgcccca
    13021 aatggtgctg ggctaaatct tcttaagtcc ctatacaatt caaagcttgt aagttctgtt
    13081 tctccactga acctttgtgt taaattagct tgatatatat ctccttgtct tatgtactct
    13141 ctaacacttt gaactgcatt tttaaattct tcctttgtaa aattagattt aagtcttata
    13201 ggtgttactt ctttttcttc ataacatatg ctatctatac cttttttctc ttcttttaat
    13261 attctttgtt ctatatcatc tattatcttt tcttctaatt cgatatctat gtttggagta
    13321 gctatataag ttttttgcac aagatggtct ataactatta catgattata aaatccaaaa
    13381 tacatatcag gcatctcaat atcatctaca gcagttctag gtaaattttc tatgtaatta
    13441 cctaaatcat aagataaata tcccacagca cctcctacaa atggaagatt agtatcattt
    13501 acaaccctat acttatgtag ttcttctttt aaaacttcta aaggattttc atcagtatct
    13561 ttatacttta ataccttaaa tggttggcta cttataaaag aatatcttcc caatttttct
    13621 ttatccatag cactatctaa tataaaactg tcatgttcat ttctaaaaat agtaaatatc
    13681 tcaaaagaat ttaatttagt atttatctct cttatcatat tacatatgta ccctttctct
    13741 agcttctgtt ataaaatttt taagcatttc atgaccatac tctgacaaaa tagcttctgg
    13801 atgaaactgt actccttcta ttaaatattt cttatgccta attcccatta tttccccttt
    13861 atcagtaatt gctgtaattt ctaattcttt tggaactgta ttggagtcaa ctattagtga
    13921 atgataccta gtgacattta aaggattttt aagtccccta aaaactcctt tattagtatg
    13981 gttgatacta tatacttttc catgcacagg ctgttgtgct tttataatat ctcctccaaa
    14041 aacatgtcca atagtttggt gacctaaaca aattcctagt ataggttttt tccccttaaa
    14101 atgctcaaca atatctatgc aaataccagc ttcttttgga gaacacggtc ctggagataa
    14161 cacaattacc tctggattta gttcttcaat gtcacttatt ttgatttcat tatttctttt
    14221 tactacaact gcttccccta attcttctat atattggact aggttgtata caaaagaatc
    14281 ataattgtct atcataagaa tcatactgta aatctccccc ctaaaaatta ttttatcaac
    14341 tcagaaactg ttacaaattt aaaacctttt tcaatcattt ttggaataaa catatctaaa
    14401 gcttcaactg tatcattagt tgcataatca tgcaaaagta ttatatctcc attttgtact
    14461 ttattaatta ttttatctgc aatgctatta gctcctggat ttttccaatc tttcacatct
    14521 acacctgtcc ataaaacaac tttatagtca tgcttttttg caatttctat taagtctttt
    14581 tctctataac ttccgaatgg tggtctaaat aatgtaggtt ttttcccagt tacttccttc
    14641 aaaatgtctt cacacttaac aatctcctct tctatctgac tagaacttaa attacttata
    14701 tcaggatgat tgaaagtatg atttcctatc tcgtgacctt ctttacttgc tcttactaat
    14761 ggttctttat accattttgc atgttttcca gctataaaaa aagtagcttt tacattgtat
    14821 ttctttaata catctaaaac ttgactagtt tctttaggat gaggaccatc atcaaaggta
    14881 attgctatta gctttttttc ccttgaacca tgtttaatca aaacatttcc attagtatcc
    14941 atcatcaaag cagaaacttt actaaaatta gatattaaca ctattccaca aaacaaggct
    15001 actgctatta agcacttata tactgtcttt tttttccatg atgtcatttt ttaacttcct
    15061 ttcaaatatt ttagttattt ataaaaattt tgagtgtaat atgttttata gcttcctcca
    15121 aatattactc caactccaat attattatag ttccccaaaa tattttttct atgtccttca
    15181 gagttcatcc aagcttcatg cgcatatatt gcattaatct gtcctgctgc tatgttttct
    15241 ccagctgaag tatatactat accttctctt ttcattctgt caaatggagt ttcattcttc
    15301 ttatttacat ggtcaaaaaa attattatct ctcatatctt cactatgttt tcttgaactc
    15361 aaagttgcct gttcactgta tctcaatgag tttaaccctc tttgatttct aacactattt
    15421 acaaggtcta taacttcaag ttcaaaacat tttttaagtt cttcgctatc atctggatat
    15481 aaatttttaa actcatcttc agtgctttta tcaattatta aatatgaaca aactctattt
    15541 tcttcatgaa tatcgtagaa aattgtaata tacttacctt ccttacttat tatatcatat
    15601 tgattttctg agtttattat atatctggta tttccttttc tcttatattt taatggtgta
    15661 atcttagttc tcactgtttg tctatcttga tttagtttta tatcttgatt ttcacatgaa
    15721 ttaatagaat tactataaag tgctactact tcatcatttt caagtccaac cattgcaaat
    15781 tcttttccat attgattata tacataccaa tcaaaaccat attcactaat atcaattcta
    15841 ccaggtttgc cgatacttga taaaacatag cttaaattat ctcctaagct tattttttca
    15901 aaattcttta tatctgactc tcctaagttc tccccttcag tattatttga ttgtaaattt
    15961 tgactttttt cttttttatc tttcttagaa ttatcattta tatttatttt gtctaaaaat
    16021 tcattccata tttcactttg aaaaaacttt tcttttaaat catatccttc ttttaataat
    16081 tcaggactat aaaataaaac aataagtact actaacacta aagcaattaa tctcttcaat
    16141 atatctctcc ttcttaaaat caatcaaaat tttttaatat tgcatttatt aagttaatat
    16201 tgatagtgta tactaatcaa tatcattcat acctatttac agtaagtatt agaaaaaagc
    16261 tttatctact aattagataa agctacagtc tcaaggaaaa tatttcatct aatgttctat
    16321 caaatataga tggcttatca aatttccaag tcccttcata aaccaattca ttatttagat
    16381 aaaaagttcc ttgcccatgt tttttaccat gcataaattc acctatatac tttttacctt
    16441 tttcccatat ataagttccc ttaccatgaa tcaaattatt cttccattct ccttcataaa
    16501 gtacttcccc tttactatta tagcaaatac cgtacccttc catctttcca cctttaaatt
    16561 cacctgcata tatacatcca tcactccact tataaatacc ttttccatct ttttttccgt
    16621 ttttccaagt tccaatatat tcatctttac tactatataa catatttcca ataccgctca
    16681 tcatatcttc ctcaaaataa cctatatatt catttccatt tgaaaatata aagtttcctt
    16741 ttccattttt tttatcttct ttaaattctc ctatgtactc cataactgtt ccttcatcta
    16801 caaaaaaagt atatgagcct ttcccctcca tctttccctc taagaatttc cctacatata
    16861 catccccatt tgaaaattta tagattttat tttttggttt ttttgtatcg acatcatcta
    16921 tatcgcgtat tgtttttcct tccaattcta atacttcatt ttccggagca aaatcgcccc
    16981 tttttaaaac acgtttcttc atgttcttta tacttctaat ctctttgttt acttcattta
    17041 tatatttcaa ataattatca tacaaattaa catattcttt agataaattc ataatgccat
    17101 tcctccaacg ttattcctag gtattttact aaatctatta aatttattat atcctaatag
    17161 tggtcatgtt ttctacatat taagtaattt tttcaatttt aattttaaaa tgatatcttt
    17221 taaagtgata cacaaatcat taataaaacc tttattttcg tatactaaat ttaaataagc
    17281 tgagagatat aatatacaaa tttacattat aaactcccag cttgtaaaat tttctataaa
    17341 tcttttgctt taaaccctct tccctttact tctgtaacat catttacaga tataaaggcc
    17401 tttttatcat acttcaaagc tatatctttt atctttggta tctcatttga tgaaactatt
    17461 gtatatataa tcttcttttt ttgatgagtg tatgcacctt ctgcatctaa aaaagttaca
    17521 cctctaacta aactcttcat tatatcttct gctattaaat cgacttcatt tgacattacc
    17581 ataacagatt tttggtaatt cattgcatct ttaataaaac tcattgaata cgcatttaga
    17641 aatagaccta taagagtgta taatgctaaa tccaacccaa ataaaaaact tcctgtaagt
    17701 actatcaagc catttattgc taaagcagtg tctttcattt ctatattctt cttaattttt
    17761 agtattgcag ctattatatc tgtaccacct tgagaagacc tagttctaaa tacaactccg
    17821 actcctaaac cagataatat accaccatat acactttgaa gcaatatatc atgtataggt
    17881 ataatattac ttatttcctg agttgctcct aataataatg aaaatacaat catatttacc
    17941 aaacttgaaa tacaaaactc tttttcaagg tatatgaacc ctaagataaa tattggaata
    18001 ttgattaaaa atgttaataa acctacattt atatttgtca aatagtttat agatgttgct
    18061 atacctgtag cacctccact aagcagatgt gctggtctta agaacccatt tattcctata
    18121 gatgtcatca acatcccaaa tagcattgtt gaatatctta aaactggatg tttttgtagg
    18181 gtattcttaa cttcactcat ttctttctcc cttcaatctt tctaaatctt tagaatttta
    18241 aaagcttact cttacatata ttattcctga tgtaatagat aatatttcaa ataaattttt
    18301 tttataattt cttgaactgt catctattac atcatctatt atattatctt tttcatatat
    18361 gtctagtgca atcttttata ttctaatctt taaaaacgtt caaatcatct catttatgcc
    18421 ttaaatgaaa ctttttctag cataatatga tactgcttat tatttaactt gcaatatagt
    18481 aaatttatag tatttttttc taaatatcta ttttagcagt aatttatttt attttagcaa
    18541 attcaagttt agtaattgtc tctttataat taattcctaa atgaaatttt tctttttata
    18601 atttcagtac atgatttttt tatttcatta tttgcatttt tttcgttcag tttttatttt
    18661 aaattttttt tattttattt tgttttaagt attcaaggta ctcatcttaa ttttattttt
    18721 tatataaaat tcattataat tggaattagt attactgtaa ttgtaccaga gactccaata
    18781 gaaacaccac tcatagctcc ttctatttct ccaagttcaa aagcctttgt agttcctaaa
    18841 gcatgtgcag atgttccaag agcaattcct ttagcaactg gatgatttat attccccagt
    18901 ttgaatactg tagaagcaac cataccacca aaaatacctg taaaaattat ggctacaact
    18961 gttattgatt ctacaccatt taaagaattt gctaaagata ttcccattgg tgttgttata
    19021 gattttggaa ttagtgaatt tattatactt acatctgaat tagctatttt gccaattatc
    19081 aatacagata caaaagaaat cactatacca caacctattc ctacaataat ctcaaacata
    19141 tgctttttaa ataattcaaa ttgtttataa agtggaactg caagtactac tgtgacagga
    19201 cctaaaaaga aatttagact atcagctcct atcttgtaat tctcataagg gattttagat
    19261 atgcttaaaa acaatattat tcctacgatt gcaattaata aagggttaca tataggatta
    19321 cttgtttttc gttgaatgta tcttcctaaa ttgaaaaaaa ctattgttac tattatacca
    19381 aatactggtg tctgtaatac attatacatt tttttgtcct tcttccttct tttttatcat
    19441 tttctccaca ataattccag tagtatacat tactattagt gtacttaaac atatgactaa
    19501 taaaagcact aatacatttt cccttattag gtctaatgac tttataagtg atacacctgc
    19561 tggaataaaa aatatagcca tattatcgag gaaaaatcca cttacatttt ctatactact
    19621 caagtctaaa actttaaatt gtagtagtag aaataataat agcataccaa ctatactccc
    19681 aggaataagt ataattcctt gtataaagga acttatatac tctcctatta cccagataga
    19741 taaaattatt accaattgat tgaaaacttt cattttccac ctccaaaaat taaagagatt
    19801 gcttcaaatc atatatttgt agttttatca acaagcaaat atctttcttt aaaatagaaa
    19861 gttataattt gacacaatct ctttttaaat ttatttttat tttaatttaa taaaatacat
    19921 acttggttgt ttcaaaaaac ttaactatat aaacctattt ttcacttaaa atttctttta
    19981 tttttgaatt gttatacata ctgtctggaa cttcacttct caaactataa aaagtctgtt
    20041 gtgcattttt taaatcgcca ttatcataat aaaacatacc caactcgtaa tatgaatcat
    20101 cataatactg gtcattttta gtataagtat ttatatattt tttgtaatat tttatagctt
    20161 catccttatt tcctaataat ttatttgtta ttgctaattg atatattgac tcagaaatat
    20221 attttttagt tttaccagaa gaaactacac ttttaaaatt atctatggct ttttcatatt
    20281 ctttgttttt ctttaaattt agagcttgat aatataaatc ttcctcatca gctacagtta
    20341 tctctctttc tggttcttta tttttaacct catttaattc cttattcgtt ttttctaatt
    20401 ttttactcgt ttctcctaac tttttatcag tttcacttaa cttttcctct ttactactta
    20461 tttggttatt caagttttct attctctttt tgctttgaat caaatatatc cccataccta
    20521 ttaaaacaca gccaactata atatatggca aaatactttt ctttcttttt acttttttta
    20581 tttcttcctt tttacttgta tcttcttttt cttcttcatt tttttcaaat acaggattat
    20641 ctttatctag ctctttaagt ttgtctaaat atccttctct cttttttaca ttacctagtt
    20701 tatcatataa caaagataaa attacatatg gctctattag ttcaggttct tcttcaatta
    20761 tgttctctaa aatatgtatt gactcataat ttaattcttc atttataaat cttatagaat
    20821 gattgtatct tcctaaaaaa gttttaaaat cttcagatga aattagattt acatattttc
    20881 tagatatatg attattttca taacataaag cagtataaaa actctcaaat gatttagcaa
    20941 aatcacattt taataatttt aaaagacctc taagatttag tatatcaatg tctttaggat
    21001 ttatctccag agctttatta accatattaa tggcactagt tatataattt ttatatgcta
    21061 aatccaatgc attgttatat cttttatatg attctaattt taactcctta gacgttgtat
    21121 ctgactttga atttaaagct ccttgcaagt ttaatagagt ttcttcttta taatttgtag
    21181 tcacataact ctgttttaat gcttttaatg tttcatcacc gtcttcttct tctatattaa
    21241 aaaaagtcaa atcactttta ttatcttcta tcaaagatat atctaaaatt tttctaggca
    21301 tgctttctat gtcttgtttt atatctttaa ctaaaataat actcacctcc actctaaaat
    21361 atatatttaa gataacatat ttaatatgtc aagtcaaaac tatacatatt tttccaccat
    21421 tctcataata aaaaaggtaa tcctcttctt ttcataaaaa ggttacctct ttttcaatac
    21481 tattaacttt taatgttgaa tttttttact tttctataga gtgtagctat acctatattt
    21541 aatgcctcag ctactttttt cataccttct gtattacttc catatttctt tatggcattt
    21601 tctatagctt ccctctcaag gtcatctata gtcttaactt cttgtttata aagttcatct
    21661 agtttaaaag atattaaatc agacatactc cttacaaaag cagttaactc cttatctttt
    21721 gaaagtatct tttctttttg ttcttcatta aacgcagcta tacccaagat tccaagtata
    21781 tgctctccca ttttaacggg agtacatata tcagctaact ctttacaatt ttctctattt
    21841 gcacatttat tacagttctc ttctatttct ttatttataa cataggattt cccatctttt
    21901 aaaacaagag agtacactga cttctctgat attgtctctc ctattttacc tctgaatatt
    21961 cctgtacctg ctatcctagt taaatctttt gtaactaaag taacatctac atccaagaca
    22021 ttagaaatat tctcgcaaat actttgaacg taattcgtta tattatttaa attcaaagta
    22081 atccctccta tttagattta tattttttat tactctacat aaatcctatt cttgagaact
    22141 ttaagcttgc ggttgtggtt gtgtctcatt aggcttatta tcagttggtg gtgtttctgg
    22201 tttttgttga ttatttgggt tttgattgtt tgtattttga ttattcttat tttcgtctgt
    22261 atttggtttt tcctctggtt ttggttccat agtcccaact aatataatac cttccttctt
    22321 aggataatat gaattagcta ttttttcagt tttaattttt ttaccatttg catcattaaa
    22381 tgttctatat gttgatacag aatatgcatt tcttccactt ttgtctactt tttctttacc
    22441 tttaggcatt gtagggtcat taactctttt cattgtagtt tgtgaaacgc catctaattt
    22501 tgttgatatt gttatatttt gtttatcttc tgcacttcca tatatattgc aaacaatttg
    22561 atttcctgaa acgtaatttt ttatgtaaac tggatgcttt agattatttt taaataagaa
    22621 atctattcca ctatctgcaa cagttgcatc tcttcctttt ggaacataac ttgatggtat
    22681 agtatgattt tttacattaa caatttctaa acctgcgtat agcacagaat tgtataacgt
    22741 agatgaaact tgacaaactc ctccaccata atcttcttcc atctcacctt ccattattac
    22801 aggtgcactt ttatatccat tttccttact tcttttccct gtatgtttat taaatgagaa
    22861 agtttctcct ggcataagta atacattact tatattatct gttgctaatt ttatgtttat
    22921 acttctattt atttgagaag ttttaaatgt tgttgaatac gtacctaaat ttgtatctat
    22981 attagcaagt tgctcttttt ttacttttgc tggagtagct tttactacta actcattttt
    23041 atagttacct tttttagttt gattatcaaa attctccata ctcttagaaa tatccatttt
    23101 aagaccatca gaatctggta taaccttgac tttttcacca cttatatcaa gtgtagcatc
    23161 ttttacatct acatctattt ctttagctat cttttccatt tctgctttta atttattttt
    23221 atcataattt atcgcaacta ctaagttgct ttttttgcca aaatttgctg aaatagtttt
    23281 tgatagattg ccaaagaaag cactctttct atttaaatta tatgcgtttt ctactgtttt
    23341 atccaggtca gtagataaat ttaaatcttt gctctttact ttccagcttt tatcattgta
    23401 attaaaatca acattctcta atttgtattt tttagctaat tcctgttttg cttgtgattt
    23461 agttaatttt ccaatattta ctccattaac atatgtgttt tttgctattt tctccccttt
    23521 aaattgcatg cacattatgc ctataaaaat tattactact aatgctacag ctccaatagc
    23581 tataactagc tttttgttca cattactcat cccatacttc tccttacaat aaaattattt
    23641 atatatccct attattatat tactacataa tgcgccttaa aaccccaaaa actttttaat
    23701 tatatattaa tttaatatta ttaattaata ttcatttagt ccattttctg atatggcata
    23761 tattttttca ttgtaaatat taatagcata aacttctttg cctctaaata agcctttatt
    23821 tactacagca taataataaa tatttttata ttttccaaac aattcttcta aactcttttt
    23881 actttttaca tatctatctg ctttctctat atctaatatc tcaatctttt tatacttatt
    23941 ttctttttca tcaagagtat tagttaacac aaacttagct ttatcttttg taataccaaa
    24001 ttgataactt ccatcttcta atattctaaa gtatccatca ccataatcta ctttgtaatc
    24061 ttttttacca tctttatatg aatataaatc tttagttttt gcacttacca aaacttcatt
    24121 tattaattta tcatactcat atacttcagc ataatagtat ttattatttt ttatcatttg
    24181 tggaagtgaa tttatagata gatttgcctt gtctttgcca ctttttttag ctattacatt
    24241 tataatgtca tcagatgtta aagtatacaa tgataaattc tttttatatt ttttttctaa
    24301 actctgtacc ttttcatcat ctgaataatc gtcttttaaa tactcaatat attgtaatgc
    24361 tctttcataa tctccagaag tatttgcctc ttcagctcta cttatatagt aatcatacat
    24421 ttcttcaata cattgttctt tcccattttg agccatactg taatactttt catcttcttt
    24481 taatacttta ttatactttt caatagcttc ataatactta tatgcttttt ggtcttttac
    24541 tgctgagtca taaacatctc tagactcttt gattgtttct atattctgtt tgtatacatc
    24601 taggttacta cccataccat ttaatattga aacagtactt atgtagttaa tggcaatatc
    24661 ataagtagta ttttcttctt tatacatttc tctaactcta tcagcttgag ctaataagtc
    24721 atccacatct atacttattt tgtttaattc tttcacggta tttattaatc caataaaact
    24781 ttcttgactt atgtctccat ctaaatattt atcaccacta tttaatagca atttatttat
    24841 ttttttagaa acagacttat taaacctttc catttttgaa tcagaaaact ttttttctat
    24901 acttgtgtag taatcaacag ctatagaata ctgtcttttc tctataatct ttaacaactc
    24961 attagcttca tttcctacac taaactcgtt actacttaag aaaaatacta ttatcaaagc
    25021 aattacacct aatgttgata atgaaatcgc aactatagat ttcctttttt catcaataaa
    25081 atctacagcc ttctcaaata aatctctaaa attcattata gttccctctc catttaataa
    25141 tctttatatt ttatggtaaa ctaaatttct tattggttat tatatcataa ttcttgaaaa
    25201 aaaataaaaa cacaagttaa atcgtgtttt tattttacct atcaatcatt attttataca
    25261 taaatatttc ctttacataa attatgatat ctttcttttt atatccctag aatgactctt
    25321 ttgtttttta ttattactga cagttttcgt tcttgtcttg tgtatagctt tgctatcaaa
    25381 tatttttcta gcattgcata aaacaattcc aattgttaaa caaactgtaa gcacacttcc
    25441 catcaaaaag taataatcca atcctatatg tactcccata actgaattta ttaacactaa
    25501 tgcaaaagtc attgcaaaac atatcatcat ggagataact aaaattctta gaattttttt
    25561 caatccactc acctcgtata atcactaaaa ttgacactac cccttatcca ttactatacc
    25621 aaattagtaa aaaaatagca attactattg tcatttatat taggaaaata ttaaatcttt
    25681 ttaataaata tctttttttg acataaggat tacttaactt ttaataaaat agtaacacta
    25741 atatgtatta attaatagaa taaaataaat gataatttat tgaggggtga tttacatgcc
    25801 aaatttgcca agtttagggt caaaggctcc tgattttaaa gccaatacaa caaatggtcc
    25861 tattagactc tctgactata agggtaattg gattgtttta ttttcacatc ctggtgattt
    25921 tacaccagtt tgtactacag aatttttatg ttttgctaaa tattatgacg aatttaaaaa
    25981 aagaaataca gaactaattg gtctaagtgt tgatagtaac agttcacatt tagcttggat
    26041 gtataatatt tctttactta caggtgtaga aattccattt cctattatag aagatagaga
    26101 tatgagaatt gccaagttat acggcatgat atcaaaacca atgagtgata catcaactgt
    26161 tcgctctgta tttattatag ataataatca aattctaaga acgattcttt attatccact
    26221 aactacagga agaaatattc cagaaatact tagaatagta gatgcacttc agactagtga
    26281 tagagataat atagttactc ctgcaaactg gtttcctgga atgccagtga ttttacctta
    26341 tcctaaaaac tataaggaat taaaaaatag agttaacagt tgtaataaga aatattcatg
    26401 tatggactgg tacttatgtt ttgtaccaga taattataat gatgaagaag tgagcaagaa
    26461 aattgataat acttgtagct ggaaaaaaga acatactaaa aacattgaaa atgaatgtaa
    26521 ctgtgaacat gaacatcatg actacctgaa caaagctctt gactgtaaac aagaacacaa
    26581 gactgatatt aaagatgatt gcaatcatga gaaaaaacat actaaaaata ctaacaaagt
    26641 tcacaactcc aaacaagata agtttaaaga taagtcttgt gatgaaatga attttaacta
    26701 tgataaagat gaatcttgtg acaaaataaa ttctagctat aacaaagaag atagtagtta
    26761 tgaagatttc tataaacata attataaaaa ctacgattat actagcgaaa aaaatactaa
    26821 aaaaatagct atgaaaactt taaaagattc taaaaaatta gttagaccac aaataacaga
    26881 cccatacaat ccaatagttg aaaatgcaaa ctgtccagat ataaatccaa ttgtagcaga
    26941 atatgttctt ggaaatccaa ctaatgtaga tgctcaacta ttagatgcag ttatatttgc
    27001 ttttgctgag atagaccagt ctggaaattt gtttattcct tatcctagat ttttaaatca
    27061 attacttgct cttaaaggtg aaaaacctag cttaaaagta attgtagcta ttggaggttg
    27121 gggagctgaa ggattctctg atgcagcatt aacacctaca tctagatata attttgcaag
    27181 acaggtcaat cagatgataa atgaatatgc tttagatgga atagatatag actgggaata
    27241 tcctggaagt agtgcatctg gaataacatc aagacctcaa gatagagaaa actttacact
    27301 cttactaact gccataagag atgttatagg agatgataaa tggcttagtg tagctggaac
    27361 aggagataga ggatatataa attcaagtgc tgaaatagat aaaatagctc ctataataga
    27421 ttattttaat cttatgagtt atgattttac agcaggtgaa acaggcccaa atggtagaaa
    27481 acatcaagca aatctttttg attcagactt atctttgcca ggatatagtg ttgatgcaat
    27541 ggtaagaaat cttgagaatg ctggaatgcc ttctgaaaaa atccttcttg gtataccatt
    27601 ttatggaaga ttaggtgcta ctataacaag aacttatgat gagcttagaa gggattatat
    27661 aaataaaaat ggatatgaat atagatttga taatactgct caagttccat atttagttaa
    27721 ggatggagat tttgcaatgt catatgatga tgctttatca atattcttaa aaactcaata
    27781 tgttcttaga aattgtctag gtggtgtatt ctcatggaca tcaacttatg accaagcaaa
    27841 tatactggct agaaccatgt ctattggtat aaatgaccct gaagtattaa aagaagaact
    27901 tgaaggtatt tatgggcaat tctaatagaa tgtaggaaaa ttttatagtt atataaaaag
    27961 cagattttat tatattaaat aagttgtatc taattaatac tattattaat tgtttataaa
    28021 ttagatactt attttttcat aatacaatct gcttttttaa tcaaaattta tttaattata
    28081 agttgtttaa ttacaagttg tttaattaca agttgtttaa ttataaagta ggaatttaga
    28141 tatactctta taccatgtaa ttatgtataa atattttttt attatacacc aatagctttt
    28201 ttagctaatt tatctgcttc ctcattatac ttatttccag aatgagcttt aacctttgta
    28261 aattttacat taagcttatt cttaatactg tcatagaaat ttttatatgc tatagttcct
    28321 tctttatttg ttttccaaac tccagtacac cacttttcaa tcccttcata gtcaaagtat
    28381 aaaatcaagt tttgaacatt attgtcaata cagtactgca ttgcaatttt agaagcctct
    28441 atctctccag ctacatttct catacttact aaagttttct cttttccttt ttcactataa
    28501 gtttttataa cttcattatt ttttaaaatc acaacacccg aaccatacat ttttactgaa
    28561 tgttcataac ttccatctac atatgcttct ataaactcct tatcttcttg aaaactcaat
    28621 ttttctgacc catcaatata ttctttagct tcttgaaaag tagaaaaact cttatattca
    28681 gctcctgaga atccatttac ttgttttttg cactcatccc atgtattata tacacctata
    28741 tttttacctt ttttcacagc ataaaacttt tgttttgcca aatatatcac tcccactctt
    28801 aatttttatc ttcttctctc ataaatatat ctaaatttat aagatagttt ctatcaaaat
    28861 attttacttt catatctttt tttataatat caatatggta cacttctcct tttacagctc
    28921 tttgtcttat attttcattt atatcttttt ttataatatc cttatcaaca tcattgttat
    28981 ccattttagc atcaacattc aacgctttta tagtatcaaa tataacagat tttagtatta
    29041 aacttctata tttatttaaa tgtcgcctta tataagtata aaactcattg ctattaacct
    29101 ttgaaatttt acaatcgtgt aaatctttct ctaatctctt taacacactt atattagttc
    29161 ctacatcaaa aatcacaaaa catccatagt catatttgct tcttaaaatt ctccccatag
    29221 cttgctttac ctttatggtc atttgaggat aatttatagt atagtagtct ataccatact
    29281 tcttcattat agtaaaatac aaagggtctt ttggatttag attcggaagc ttgtctaaag
    29341 ttacacaaac caaaccatcc cctggaatgt ctacgccttc aaaacatcct tttgagccaa
    29401 gtactacaca ttttctattt aaatcgttaa ggtgtctaat ccccttttta tcagcataaa
    29461 tttctaagtt aaaactatgt aagtattttt tcaatatttc ataagttttt tcttgtctat
    29521 ctttactatt aaaaagagct aatgtatgac cttctgtaat ctttgatata ttagatataa
    29581 ttttactcat ctcatttgga aattcagaat ttctgtactc acaaatatca gaaaatccaa
    29641 ctacagagac tctattttta taatcaaaaa ttggttctat aatcttttcc acattcttaa
    29701 ctctgtctat tcctaatgta tttttaaaat aagacatact ttcagataaa ctcaaagttg
    29761 ctgataaaaa tattcctttt tctaattgag ataaaatatt ttcttcaaat aaatctgcta
    29821 tttttaatgg cactactcga aattcaaagt cattataatt cttatcaatt tcaactatcc
    29881 ttgcataatc atccttttca tcatactcta aaaagatttc aaatataatc tttatatcct
    29941 ctaaatctct tgtctttgcc tttccaaatt tatatatatc tgcttcttta tctatactat
    30001 catcatcaat attcctataa attattatta ttattgaaac taaatttttt attattttct
    30061 cacaactcaa ctttatcttt tcactatagg ctctataact tatttcagta tctataccat
    30121 ccttttttag ttttcccaca atttcgtcta tttgcaaatt cagttcccat cttaaattat
    30181 aattacttac attattatat tcattacaat taccaaactt taatattgaa tctatctctt
    30241 caataatgag atttatactt ctagcaattt tttgtttatc ttctcttcca atttttaata
    30301 cattataaaa tttatcaaaa gcctttattt ttctcatatt tctactatat tttttatata
    30361 taaagctact attttgaata aactcgtaag gataaatttc ttgtaataaa taccttaaag
    30421 actttgaatt tataatgctt gaaaagaaat catatccttt ttcagtcaaa ttatgtgctt
    30481 catctactat tatattttct aaaggttttt catccttata tggccattta gctaatagtg
    30541 aatgatttac tactgttata tgttcttctt ttaattcttc tattctgttt ttatataaac
    30601 aatctttttt gcatttttta ggtctgcaca tatttgggtc acaacttaca tttcttaagt
    30661 gagtacttat ttccttgaag ttgtcaaaca ctaaataatt tatttcttct atatctccat
    30721 acttgccacc ttcaactaat ctcttcaaaa atattaaaga taaaatttct cctttagtag
    30781 ggttttgaga ctcataatca tctatatatg cttccagtct atctatacaa atatagttat
    30841 tttttccctt tatatatccg taacttactt ttccatttag acccaaagaa tctaacacat
    30901 ttggaatatc tttatttatt aattgtatct gcaattcctt tgtgtcagta gaaattaata
    30961 atcttttttt attaattcta gcctctaata ttgcaggcaa taaatatcct acacttttac
    31021 ctataccagt tggtgcctct atacatgcaa ttttttcttc atcttcgcta tttctaaaca
    31081 actctcttat tgttttagta agctcatatt gaccaggtct atattcatat gtaaatcctt
    31141 ctttattctc ccaaatagtt ttgtatttta ataattcttc ataatttttt tcttcttcat
    31201 ttaaaatttt taaggtttct ctttccttct tcttttcttc ttttgagtca aatatattat
    31261 attctttttt tatatttata ttatttgata aatcataatt tgcctcttct aaaaatttgc
    31321 tccattccca tgttggaaga ttaaattttt taagatatga atttatttta aaactcaatg
    31381 gctccaaagt agttttttca tagttattaa attttactaa aaggcaattt acaatattta
    31441 ttatatcaat agcatctgat aaagctctat tttccacttt agaattacaa tttgttaaag
    31501 tattttttaa gtattctaag ctataatctt tatgatatgg ttctaaaata actgctaatt
    31561 caattaaatc aataaatttg ttttttaact ttggcatatg atactcaaga aacttttttt
    31621 tcagacttaa atcatgacat ataatgaaac tatcttctac aaaatttaca agtctatctt
    31681 ccaccagttc tatttcatta gctatttcta aatcattttt atctaaattt ttacatactg
    31741 agaatacttc tacaggtacc tcaaatttgt tttttattaa tgattcatag gtatatattt
    31801 tccagtcttt aaccttgaca gctccaactt ctaaaatttc tgaatttaaa caatctaatc
    31861 cactaacttc tatatctaaa aaaacaacat cattcaatac acttattata ttatccattt
    31921 ttatatccct tcaagttaaa ccagcctaac ttatttacaa tcatttctct ttatatttta
    31981 tcatttatat acactttatt taagataatt aatatcaact ttatatctta cagtaaactt
    32041 ctttcattta ttatttgtat atgtgaatat ttttaactat aaatattcaa actatattat
    32101 aagaaatata ctttaaattt taaataagag gtgaaattgt gaaaaaagat agcctaaaaa
    32161 aatacattat gataggagca tttgctctaa tactttttgg aattgctagt ataaatttta
    32221 aatcattaga taaaacaaaa actcaaattt ctagtccaac actcgacaca catgaatatg
    32281 attggtattt taaccctagg gaggatggta aacagccatc acctataaaa gaagctgatt
    32341 tttttaaaaa atacggagct tattatgtag gaaatcccaa tgaaaaagtt atatatctat
    32401 catttgatgc tggatatgaa agtggaaaca ctccaaaatt attagatacg cttaaaaaac
    32461 acaatgctaa agctcaattt ttcgtagttg aaagttatat aaaaagcaat ccagagttaa
    32521 taaagcgtat ggaaaaagaa ggacatttag tttgtaatca ttcaaaaagc catccatcaa
    32581 tggcaggtat tacagatttt gaaaaattca aagaagaaat tacaagtgta gaaaaagcat
    32641 acaaagatgt tactggaaaa gaaatgccta agtattttag acctccaatg ggtaaattta
    32701 gcgaacaatc tttaaaatat acacaagatt taggatataa atcaatattt tggagttttg
    32761 cttatgtaga ttggtatgaa aagaaacaac ctactcatga atttgccaaa aacaaaattt
    32821 attcaagaac tcatcctggt gcaatagttt tactgcatcc aaattcatca acaaatactg
    32881 agatattaga tgaggttctt acacattggg aaaaagaagg ttataaactt aaaacacttg
    32941 attatttaaa taataaaaaa tagtatcata aatttaaaaa aactctatca tgaattatag
    33001 taaaaatata gttatttata aatactttaa ttctcgatag agttttttat tttttatagc
    33061 tatttaaaaa gaattgtcca ttcatttatc tctttgtatc ctatattatg atatatttta
    33121 cctgctacat cattattata aaaaagacat ggaacttttc cttctaacaa caattcttta
    33181 ctcaaattgt aaaccatata acttgcaagt ccttttcccc tatatctttt atctgtactt
    33241 acagagacca ccattgccaa aaaactagtt tccataccag ttgacacagt gctaatcata
    33301 gtatcatcat ttctaataaa atatgctctt acatttccct cttttaaatg aacttttctt
    33361 tcttctatgt aatttttact tactttatag tctgacctat ttaaaagttt tcctatttca
    33421 tcaatatctt tttcctgagc attttctatt ttatactcct tcatgttgga aaaatctatt
    33481 tcttttattt cttttaatac acaaaattta ttatctagtt tttcataaaa atctttgtat
    33541 ttacttatta gtctatctat tacacatttt tttccactta tgctttctac atctaaatct
    33601 tttatatgat tcttcatttc tcctatatta aaattatttt taaaactata aattataagt
    33661 gttgacttat tttttaatat tatagaggtt atatttccat cttctacctg agaccatata
    33721 tcttgatatt gagagctaaa tccataattc tcaacgtatc caattatgaa taaatttaaa
    33781 ctttcttcat gtgatacata ttgccaaaat tctttttcta aagtattatc tatttttaca
    33841 atcaagcaga tatcctcctt ctatgttaat aatgatatac cttataattt atagtatcat
    33901 ttaaaatcat tatattctac caaatattct aaatttttaa atatctatgc aatatgttcg
    33961 tatttatttt tatattctgt tactgcttca ctccaagctt cttctactga aatacctctc
    34021 ttatttgcaa ttttttgtgc taattctatt attttcatag cttttcttga tatattcata
    34081 tatattcctc ctctaaatta tcataagtta ttttaaagtt aaatttctat agtcatgtta
    34141 tatatttatt tacttatatc ttactaatta tttatgtact aaatatgaac tacaatagaa
    34201 gaaatttaga acttaataaa acataaaatt tatatacagt tcatatatat ttgtcatatc
    34261 acaagacaag tgatataatt tattatcatt ttactggggg tgatatgcta tgaaaataag
    34321 agctgaatat acatgtcttc ttgaaattgt acatgacata atcaaaggaa aatggaaaac
    34381 tatccttata tttgaattaa gacaatctaa taaaacattt tctgaattgc aacataatat
    34441 ttatggaata aatcaaaaaa tgttgattca acaattaaaa gagttacgtg attttgggat
    34501 tattgataaa ataagttctg ctggatatcc attacatgtt gaatattttc ttactaatcg
    34561 tggtaaaaaa atgttaaaag cagtggaaat aatgcaagaa ataggaatcg aatatatgct
    34621 tgaacatggt caacaagaaa ttctagataa taaaggaatt tgctacaata agtaatccta
    34681 tacataccaa aaagtaagta atttacattt tttacaatag tattataata aaaataaata
    34741 ggaggtataa tcatgtctat agctttaata ttgattgata ttcaaaatga ttattttaaa
    34801 aatggaaaat gtgaattatt tcaatcagaa gaaactgctg aaaatgctaa aaaaatacta
    34861 ctgtttttta gaaaaaacaa tttaccagtt attcatatac agcatatatc aacagataaa
    34921 acagcttctt tttttcttcc taatactcat ggttcagaaa taaataaaat tgtttttcca
    34981 ctagataatg aagaaattat aactaaacac acaccagata gtttctttga aacagatttg
    35041 caaagtatcc ttgagaaaaa aaatattact aatctagtaa tctgtggaat gatgagccat
    35101 atgtgtattg atacaagtgt aagaacagca aagaaacttc gatacgacat aactctaata
    35161 agcgatgcat gtacaacgaa gaatttatct tggaataata tagaaattaa tgcaaatact
    35221 gtacatcaag tattcatggc atcactacag aacagttttg cagatgtaaa aaacacagat
    35281 aaatttttat ctaactatca caaataaaaa catacactat gttaaaagag caagtttgat
    35341 ttatttaagc ttgctcttta atcatttaaa attagatgct ttctatcatt tttatataca
    35401 taatatacgt ttatacattt tatattcata tataactatt atataaattt taaactttac
    35461 tatattcaca taaaaaatgt agtattatag tatatataat attatataga agggggtgct
    35521 agataaatga accatcatat attgattcaa tttgaatata tagcaagaat cattactgct
    35581 ggtttctgtg gggctttaat tggatatgag agaaagaata gactaaaaga agctggaata
    35641 cgcactcatt ttatagtggc attagcttca gcacttataa tcattgtttc caagtatgga
    35701 tttaatgata tagtgggtac tccaggtatt ggtctagacc cagcccgcgt tgcagcacaa
    35761 gttgtcagtg gtataggttt tttaggtgct ggattaatat ttattagaaa tcaatcagta
    35821 agtggtctta ctacagctgc tggtatatgg gcaacagctg gtacaggtct tgccattggc
    35881 tctggattat acttagttgg tattgtttca agtatcatta ttgttattgt acagataaca
    35941 cttcatagag ataggaaatg gatgaaactc cctacagcag agcaagtttc tcttaaaatt
    36001 tctaacagca ctgattccat agaatatatt gaaggtaaat ttaaaaaatc taatattgaa
    36061 attataaaca tgaatattaa acgccttgat ggggattggt tagaagtagt tatatatact
    36121 aagttaccta aaggatataa aaaaacaaat ttattaaaat tatttagcga taatcctctt
    36181 atagaaactt tagaaatata atataaaaat tgaagcttaa aaataataat atatttaatc
    36241 aaggtcaata tataaataat tacgggatga gtctatataa aattcatccc ttgttttttg
    36301 ccattcatat atattttttc aagatatttt tgttcatcta ttatctagcc aagtaactct
    36361 tgtgaactaa actttttaat acaaaaactt caatacaaaa ctaaatataa tttttattaa
    36421 atattctaca tgtatagttt atttcttgag tgaaaaatag atttcatatt gttaagatac
    36481 tgttaaatat ttcaattttc tgacaagtat gttgaacaat aacttatcct agtgtattat
    36541 tttagttgta gaataaaact aaatcggcaa aactagagaa atttagtgac gcaaagctat
    36601 agggactaac gcttataaaa taaactatga gttatgtcag ccagttgcca aaaagatatt
    36661 gttctttttg ttttatgaaa gttttttagg gggatagtaa tatatatgtt tagagatgaa
    36721 atggataaat gtacacacat gttaactgct tatatcagta gtttatatga ttattgtgat
    36781 tttatagata cacagttaga tgattttata ctagagtacg gagaaaatgt agtagaatct
    36841 tgtttacatc aagtgatggt attggtaagt aagtataatt aaatagaaat tgttttctag
    36901 agaacccttt ttagttttat ctacatttaa aaatgttatt atattaaaaa actaatctcc
    36961 cctatatgca aagttttctt ccatataaca atttcttccc atttatactt tttcacttgt
    37021 atctttaata aaattcaact tcaaatatta atatacttca aaaattattc tatttcttat
    37081 ctttacacca attcctatca gtattatcct ttatataggt tctaactatt tctccacaat
    37141 ctaaacataa atctgaatat atagtagatt gtactggaaa agttttattt ataactttat
    37201 ctaattttcc atctcctcta tattttaatc caatagtttc agcatatatg ccaccagttg
    37261 ttaaaattcc cttttcaata tttacacttc cacaattaga acatttcaca tttataaccc
    37321 cctaaacaat tattttttca tgctatctta taatttaaaa tataatttgc taccgatttt
    37381 ataacacagt ggcaaatcaa cattttttca ttctatttac catctaatcc attgaaatat
    37441 agctatttag ttataggaac ttgaatttcc gttacaaact cctctacaat tccagtctca
    37501 tgtatatcta tcttatagat ttctataggg tcagcaatta tcttataatt attttcttct
    37561 ataaatttaa acaccttctc catatatatc ttattattag aattactccc agtataattt
    37621 aaagttacat aatatccttc atcaaaaact atattatata ctttctcatt actttccaga
    37681 aaacaaaata cagatttaaa ctcattaaat atacctttat taattgcttc agtagaaaag
    37741 gcagaaccaa tattgttgtt tccaagtata ttaaatctat cttcatattc tttctgaagt
    37801 ttttgaatta aaaaatcgaa gtcttcatcc ctctttatat cagcatttaa ttctaaagct
    37861 tttctttttt ctatatatac aacctctatc tcatctattt ttgtattttg aattacactt
    37921 tcaattgaac tcaatctttt tataatattt tccttatggc ttataaattc agctattttt
    37981 ttatcaatta aatcgagttc ttcatttaaa atatttttcg tagactctat acttctgtca
    38041 tctaggtatt cttttatttt cttcattgga aagttcaagc ttctaaattc ttttattaaa
    38101 tttaatttcc atatatctga tatattatac attctatatc catttatatc tctaactggt
    38161 cttagaatgc ctatttcttc ataatacatt aatgaatctc tacctattcc atatatttta
    38221 gatatctctc ctattttata gtaatctttc atttatataa taaactcctc cgaaatctta
    38281 tttttatttt aaatcaac
//
LOCUS       DAEPIN010000034        38091 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.034, whole genome shotgun sequence.
ACCESSION   DAEPIN010000034 DAEPIN010000000
VERSION     DAEPIN010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 38091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38091
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.034"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            218..1180
                     /locus_tag="KR250_001938"
     CDS             218..1180
                     /locus_tag="KR250_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017561613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HBH0067874.1"
                     /translation="MAKIDNMMSILWMLNSDKKITAKQIAEKLEINIRTVYRYIDVLS
                     ASGVPIISDTGHNGGYSLLNNFTKAPLFFDIEEQKSLLHAALFAKKEGYFLDEALDKS
                     ILKLKMYSNQNQEDILTNHLRALEVIKPIGKVSIESVLKKLENSILKELSVEINYHAG
                     RKAKSKYRIINPYGIVHWNNNWYVVAFCNMRNEVRSFRVDRISEIIETSNTFNRPTEF
                     SASEFFIKGIIPEIKDEQNIIQLVVEGSIDSLDSLSQHWFLGHYLKERTPNKIIFMLE
                     EEVICKYIPNILLPYRKTIQVIEPLSLRKKIIEDLLELIDYYQI"
     gene            1259..1888
                     /locus_tag="KR250_001939"
     CDS             1259..1888
                     /locus_tag="KR250_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HBH0067875.1"
                     /translation="MKNRKVYMYVFDTMSDWEVAYLTAELNTGRYFKKGLKPLKVITV
                     GIDKNPIITMGGLKVLPEITVDEFNMESKDLLILPGGNTWLSMNHEIILEKVKEALIQ
                     DSIVAAICGATLGLAKKGLLDVRNHTSNDLEFLKMVIPNYSGEKYYKTESVVSDENLV
                     TASGIAPLEFTFQVLKLLDVFESEALNSWLNLYKTHDSKYFFELMNCNK"
     gene            2722..3135
                     /locus_tag="KR250_001940"
     CDS             2722..3135
                     /locus_tag="KR250_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0067876.1"
                     /translation="MSKSELGVKANLVSELMMLSYFFIDLLLQKFIFKGEGIWFTFII
                     LLAFWVCNLMYNKKYNDLVDEASKLMLLKVDQIASKIMLYSMFVIAVLFSVNHTRALD
                     ISNLDIGIMLLAVLFIQSLSKLILYIYFDKKGICN"
     gene            3205..3621
                     /locus_tag="KR250_001941"
     CDS             3205..3621
                     /locus_tag="KR250_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0067877.1"
                     /translation="MSKSETGVRSKLILELLVITYFFIAVLATKYIFKGPSTASLPGL
                     SWLIVFGCYSIYNKKYNDLVDESSEFILSKVNKIAIKIMIYSIVIISIFFATPYTSNI
                     NISNLDTGIILIAVLFIQSLSKLVLYIYFDKRGICN"
     gene            3622..3834
                     /locus_tag="KR250_001942"
     CDS             3622..3834
                     /locus_tag="KR250_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0067878.1"
                     /translation="MAILMKTKIKEYREKLLMTQNELAKLVGVRRETIVHLENGKYNP
                     SLKLAMDIAKVFDTTVENLFEFIEEE"
     gene            4878..5711
                     /locus_tag="KR250_001943"
     CDS             4878..5711
                     /locus_tag="KR250_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBH0067879.1"
                     /translation="MVIKKVFNNNVVFARDNSGQEVILMGKGIGFNKFTKDLVEIDKI
                     EKKFIFNNEESLSELHSLLDRIPLKDVELVSEIIQMGREELGDNLNDSILITLSEHIS
                     YMLKRMDEGLVFQSPLQWEIRQIYPNEYNFSKKAVEYLRKKLDKNIPDSEIAFITIHF
                     ANAQLETKDMQETLLLTKIIDNVLDIIKYHYGIEINEYSFDYTRFISHLRCFIKRQLS
                     GENTNVDTSLLDIIKMKYKSDYNCAKKVKNFLEKTYKWKISENELLYLTLHLNRLSNI
                     K"
     gene            5837..7678
                     /locus_tag="KR250_001944"
     CDS             5837..7678
                     /locus_tag="KR250_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBH0067880.1"
                     /translation="MKFERESEEIIKYVGGKENIISLVHCATRLRFKLKDTSKTNKEE
                     LLKMKDVLSVVNSGGQYQVVIGNKVTDYFETIIKKAVLQGQNTEEQGEKVSIVSKIFE
                     SISGAFSPLIPALAGAGMIKALLTILTTMKWMSDTSSTYAILSAAGNAVFYFLPIFLG
                     ITLAIKLSANPYVGGVIGAALLDPNFTALIGAKGGANFFGMPVTAIDYSFGVFPIFVA
                     IFIYYYVDKLLKKIILKDLQLFLVPMISLLVMVPLTVILFGPIATNVGDIISNIFMGL
                     FEFNRVIAGAAIGIAMPFLAVLGLHWGFTPITLQNLATMGGDPIEGAAVAAVFAQIGI
                     AIGLFMKVRKSKKNSELKVLAGSTAVTGILAGVTEPIIYGIILRYRRMIPVLAIAGGI
                     GGGICGVFGVTCNAYVFHNIFSMPVYTPFLGYILGIGTALVLGAVLSYFFGLSKEECE
                     EGNEEVAFDEDDGIEEILSPIEGLQIELSEVEDEVFASGTIGNGMAIIPSKGEVVSPV
                     NGTITTIFPTKHAIGITSDKGVEILIHVGLNTTMLDGKYYTTHMNAGDKVKKGDRIVS
                     FDKDKIEGEGYSIVTPIILTNTNDLADVIVKENKHIKIGTPVIKVVY"
     gene            7701..9134
                     /locus_tag="KR250_001945"
     CDS             7701..9134
                     /locus_tag="KR250_001945"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003243232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBH0067881.1"
                     /translation="MKSNQEFLWGGAVAAHQVEGGYNKGGKGISIADVMTAGTHTISR
                     KITDGVIEGLNYPNHEAINFYENYKEDIRLFAEMGYKCFRTSIAWTRIFPKGDESTPN
                     EDGLKFYDDLFDELLKYNIEPVITLSHFEMPYHLVKNYGGWRNRKLIDFFVNFCEVVM
                     NRYKDKVKYWMTFNEINNQSITTNPIYAFTNSGIIYEEQEDKEEVMYQAVHYEFVASA
                     KVVKIGHEINPEFKIGCMVAAMPSYPYSCNPEDMIKFVESNREQLMFTDVHVRGHYPR
                     HTLKLWERKNYNLDITEEDKKILKEGIVDFIGCSYYLTTVVTADKTMKTTGNDSAGKA
                     DVVENPYLKISDWGWNIDPVGLRFYLNQLYDKYELPIFIVENGFGAEDVLKSDNTVDD
                     DYRIEYLASHIREMKNAIEIDGVDVIGYTVWGCIDPVSFTTGEMKKRYGFIYVDKNND
                     GSGTLKRYKKKSFDWYKNVIKFNGEIL"
     gene            9839..10717
                     /locus_tag="KR250_001946"
     CDS             9839..10717
                     /locus_tag="KR250_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HBH0067882.1"
                     /translation="MLINMKEMLKVAQENQFAVPAFNIGSGQILKAVVQSANEKNAPV
                     ILAIHPNELSFLGDSFVASCIEEANKSKVPMVIHLDHGENKEQILRAIRCGFTSVMID
                     GSHLPYEENVAISREIVEIAKGLNVSVEGELGTIGTTGTSSEGGTDEIIYTDSKLAKD
                     FVEKTGVETLAIAIGTAHGIYPKGFKPELKLDLLKEIREVVDIPLVLHGGSSNPDEEI
                     AQAVKLGVCKINISSDVKSAYYKKCRELLEQNPSLYEPDTIYPPCIKSAREVIEFKMN
                     LFNAIDKLKCFYKNKN"
     gene            10753..12648
                     /locus_tag="KR250_001947"
     CDS             10753..12648
                     /locus_tag="KR250_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBH0067883.1"
                     /translation="MIGKILRSDCIVLDKQLQTKKDVINYMANHLYHGNYINNKEKFI
                     ADINKREKESSTNMGNLIAIPHAKSNVVNQPVMMYLRTDREITDNDGQKLRSFFMIAV
                     PESGSDEHLKIISKLATYLMEENFLKHLLSAETPYEILSIFNNKAAESGDFSNNQNKG
                     LIVGITGCPTGIAHTFMAKKALEEAGQELGYEVKIETHGAIGVENKLTKEDIKRAEYV
                     IIACDKEVQLSRFIGKKVLQKKVVDAKNVSLAKNLIINAENGMAKEIKPGGKTLEDSK
                     EMLQGSEKKGVYKHLMSGVSEMIPFVVIGGIGIAIAFMFGIFASDPTHETYNPIAGFF
                     GQLGGDSAFKLFIPILAGFIAKSIAGRQGFAPAMIAGFMAVIGGSGFIGGMVAGFATG
                     YIALGVMKITENLPDVLKGVNAVLICPLLIAFTSGAIMFFVVNTPASWLNEALQSWLQ
                     SMSGTNKVLLGAILAGMMASDMGGPINKTASAFGLAMFSSNIFEPSAALMVGGMVPPL
                     AISLAMLIFKNKFTKEERKSSLPTAIMGASFITEGAIPFAAADPLRMIPANIIGSCIG
                     GAICMSLDITLMAPHGGIFVIPFACSAPVAYIACILIGTVITALIIGVTKPTIRSENS
                     EVELLGA"
     gene            12916..14823
                     /locus_tag="KR250_001948"
     CDS             12916..14823
                     /locus_tag="KR250_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBH0067884.1"
                     /translation="MLVSRQIEFLKLILNENKYRPIKYFKDKLNVSDKTLQKDLKMIE
                     KYLETFNIKIDIKRGYGILIEDLAKKNIDLINSLNIQSKENKSLSVEQRRMEILKYLL
                     LNSNNITSINQLADKYYVSKTSIVNDFKYIEAWIKEYNLKLNKTLEGTKIIGKEVDIR
                     KSIAKMMDDLLDENYEQHDISELTRLDSATFSALINLFDIDSIIFVETIITELEYNLG
                     YTINQPYYINLITHILICLKRIEEGNQIESQEEREISVDNLDELVYKNVSTLIDKIEN
                     RYRVKITKDEIMYIYIFLVSSGFSSENNSSYNDESDRLSESEKVSSIMIENMSRFLNI
                     NLKNDDLLQKSLASHVRPMLNRLRYDIQIKNPLLGEIQERFSDVLGLCMMTINIMTEY
                     DNLKNISIDEVSYLATYFQAAIERNMSSKRVIVVCHTGYGTSQLLAARLKREFPEWTI
                     VDIVPMHQLSKRNLDDVDFVISTVNLDLKDKLHIVVSVLLMENDINNIKNALLSKPKK
                     SMNLDTNLLGMYLEDNIYFNFDVAQMESLIGVNLGSKYESISLGKDLNIYIHKQSKLN
                     KGIMNINNVTRTIDIYLEISNQSFLKNILMEISNLYRQKNYINYIIDCKSKEDIKILF
                     KNNLLEKTNGN"
     gene            14813..15262
                     /locus_tag="KR250_001949"
     CDS             14813..15262
                     /locus_tag="KR250_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBH0067885.1"
                     /translation="MEIREILTEESIILDLKSTTKEGIIQELVDKLYDTGSITLKSKF
                     IKALKHREKIGSTSIGYQMAMPHGKTKYVKKASIVIGKSKKGVDWPSIDGLPVKVIIM
                     FAMPQSGGNEDMILISKIAYILGGKNIGERLLNANTKKEILDIFYEV"
     gene            15362..16249
                     /locus_tag="KR250_001950"
     CDS             15362..16249
                     /locus_tag="KR250_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBH0067886.1"
                     /translation="MAIYLEMPKLIHEFPFRSLSNEGEVLTTPHWHKEIEILLITEGV
                     VNLFINDKPMQLKKGEIVIIKGGDIHYILPSPGSERLVFQFDISFFNDLILLNEEKES
                     LINLFSSIKKCSRDWSAKIRKSVFDILIDIYNEDLYKIEGYSYAIKGKMYNLIPILYR
                     QIPREIEENTVEYEINSKEILERLNNIFKYVEKNYKQPIKLEDVSYEVGFSVYYFTKF
                     FKKNTGKTFITFLNEYRLEKAKWILLNNNISIVDLVEEVGFGSTKTFYRVFKEKIGVS
                     PLEFKKRYNSLEKKGSVSK"
     gene            16652..18100
                     /locus_tag="KR250_001951"
     CDS             16652..18100
                     /locus_tag="KR250_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HBH0067887.1"
                     /translation="MKLGKDFLWGGAISANQSEGFYLDGGKGLSQFDKLPLNDKRLKD
                     VCLDEDNILNENNQYYPSHIGIKFCENYKEDIAMLAEIGFKCFRFSISWSRIFPTGEE
                     SEPNEKGLQLYDNIIKELKKYNIEPLITISHFDIPYSLVEKYSGWADRRVVNLYVKYA
                     EILMKRYKDDVKYWIPFNEMNMIMHIPYIGAGLTFIADENQLQKKYQAAHHQLLANAK
                     TIEIGRYINKEFKFGCMLAAGKTYPYTCKPEDVFATLEHERHNLFFSDVQVRGVYPSF
                     MNYYLKSNQIKLIIKEEDLSTLKDNTVDFVSFSYYSSACSSARAEGLEKSKANGPETI
                     KNPYLPKSNSVWQNDPVGLRITMNQLYERYQLPLFIVENGLGTQDIVNEDGKIIDDYR
                     IDYMKEHIKNMIIGVNEDGIELIGYLMWGCIDVTSVSEGKMTKRYGIIHVDSDDYGRG
                     SFKRTKKQSAFWYKKVIETDGEILFNDMETHA"
     gene            18126..20516
                     /locus_tag="KR250_001952"
     CDS             18126..20516
                     /locus_tag="KR250_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HBH0067888.1"
                     /translation="MSLIFTTKDQLIEQGFSKDTIKSNETLFTLANGHLGVRGNIEES
                     DFNNEYVDNMGTYVNGFYEESPITYGESAYGYAKQNQTICKLPNAHIVNFSIEGDCFD
                     LSKGITSEHSRILDLNEGILKRSFIWENSRGRKVKVSIERLVSQDIEELMMISYTITP
                     LNFNGKIKFKNKMENSMEKFNCEKVEDPRVASINKKQFKINVKKYNNKYFMNLVTERS
                     RLGLWCGFDSYITCGNITNETYNVSDECIEREIEVYAKKDERLNLEIVVGYSRVYSDL
                     NLNEERLKSLECLESLKCLESEAHLKSLELVLREACNIGFDKVKNIQRKKMNEFWTTS
                     DVEIEGDKTLQLGIKTNLFHIYQSVGRDGKTNISAKGLTGDGYEGHYFWDTEMYILPF
                     FIYTQPSIARALLDYRYSILDASRERARELGINKGCLYSWRTINGNECSAYYPAGTAQ
                     FHINADIAYGIRTYFEATNDEEFMMQNGLEILIETARFWVEFGDYIESKNNKFCINGV
                     TGPDEYTAIVNNNYYTNLMAKYNLIYAVQMVDKFKHNLFSNEVFRKVKLQEYEVKNWI
                     NAAQNMYLPYDEEKKISKQDDSFLEKSVWDFKNTPKEKYPLLLHYHPLTIYRYQVNKQ
                     ADTVLSALLFPDEFSLEQKKRDFEYYEKITTHDSSLSRSIFGMMASEIGNHEKAYNYF
                     MDTALMDINNLQKNTSDGIHAANMGGTWMSLVYGFAGMKIKNDKLSFEPRIPKHWKNI
                     KFNILFRDNLVSVNIEEYKSYYKLIKGRNITILHDNKEIKLSKNIIEVNNCFKGSN"
     gene            20518..21189
                     /gene="pgmB"
                     /locus_tag="KR250_001953"
     CDS             20518..21189
                     /gene="pgmB"
                     /locus_tag="KR250_001953"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HBH0067889.1"
                     /translation="MKAVLFDLDGVITDTAKYHFEAWHEIAKSLDIEIDETFNEKLKG
                     ISREESLDKILLSANKVDSYTKEEKLKVCEEKNNIYIKLINSLTYKDILPGINELIKE
                     LKKNNVKLGIASASKNAPRILESLGIKDEFDCIVNPSLLKRGKPYPDIFIEGSKILNL
                     KQYDCVGIEDSYSGIKAINAANMTSIGVGKKEVLGKCDHIVEDTSLLNYNTLLQAWSK
                     KINLD"
     gene            21228..22598
                     /locus_tag="KR250_001954"
     CDS             21228..22598
                     /locus_tag="KR250_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBH0067890.1"
                     /translation="MKNKYEEVAQLIIDGLGGKDNIIDLTHCMTRLRFILKDEKKVNA
                     DKLKSIDKVVGVNSTSTQYQVIIGNEVGVVFNAIINKGINTTGDIKSDIKNKNKKNSI
                     LNNIIDTITGCMTPMIPALTASGMIKVILSLCTTFNWLSSNSSTYRVLDIIGDVAFYF
                     MPIILAINAAKKFNVNTSIAVIVVGVFLHPNFSAWVSSGDPISFIGVPIQDVIYAASV
                     IPALLTVWMMSYIEKFIDKLTPSMLKTILNPTLVLLISAPIALIVIGPIGNFLGEGLA
                     SIINLLQGRLGFIMVCLLAAAMPFIVMTGMHHALTPIFISAFATTGQESLILISQVCA
                     NLAQGGATLAVALRSKDSKMKQLASAAWISAIMGITEPALYGVTLKLKKPAMLASIGA
                     GIAGLFAGITHVTLYVPQNSIMAVLGLSGEKGISNIVNGLIVIFIALVAPFIMTYVFG
                     VEKNVK"
     gene            22945..23787
                     /locus_tag="KR250_001955"
     CDS             22945..23787
                     /locus_tag="KR250_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBH0067891.1"
                     /translation="MIINKILNNNVVITLDDNDEEVIVMGKGIGYQKSKGNLIDKTKV
                     NKVFRISNKEISNKLQELLNNIPIEHMKLSSEIIEYAQIKLNKKLNESIYISLSDHTY
                     SAIQRMKEGINVKNAILWEIKRFYKEEFEIGMKALDIIENKTGIKLPEDEAGFIAFHI
                     VNAQLSEGHTLASDITKLIQEVLSIVRYHFRIEFHEESVFYYRFIMHLKFFAQRLLLD
                     SAHEGETDKELLSIIKSKYNKEFECVVKIKNFIKKQYNYILTDDELIYLTIHLAKVVK
                     DSNM"
     gene            23932..25860
                     /locus_tag="KR250_001956"
     CDS             23932..25860
                     /locus_tag="KR250_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBH0067892.1"
                     /translation="MGKYKQLAQEIVNNVGGKENISGLVHCITRLRFTLKDESIANDN
                     VLKNMEGIVTVMKSGGQYQVVIGNHVEAVYKDVVEIIGLDDSSTSSETKKSGNILDKG
                     IDIISGIFQPVLGIMAACGMLKGFNALFVAMGLYSDVSGGYMVINAAGDALFTFLPLF
                     LGYTSAKKFGLKPMLGLALGAAVCYPAIQGSSISAGADVMYTLFKGTMFASPVYIDFF
                     GLPIVSIDYTGTVVPIIFIVYFASRCEKLFNKCVPNLVKFFFVPMLTLLITVPVALIV
                     IGPIATFGSTLISQIVISIRDFSPLLAGAIVGFTWQILVIFGMHWGFIPVYINNVMTL
                     GYDNVMMPFFACTFASSAVVLAIFFKTKDKKLKEMAIPNFISGIFGVTEPAIYGILLP
                     LKKPFIISCIASGIGGAFYGFFNLRKFITGGMGIFELPAMIEPDGGMGNLIVALSGIA
                     ISMVVAFVLTMILYKDEKVEESKKVRNKGKEEIKEVKSTKLEREIVTSPIKGEVLKLS
                     DIEDAAFASGVLGQGVAIIPSDGKVVAPVDGVVTTLFPSLHAIGILSDTGVEVLIHIG
                     LNTIQLEGRGFEACIKQGDRITKGQTILNFDVYAIKELGYSTVTPIVITNSSQFLDVV
                     ETESKNIELEDNLITVLF"
     gene            25921..27348
                     /gene="ascB"
                     /locus_tag="KR250_001957"
     CDS             25921..27348
                     /gene="ascB"
                     /locus_tag="KR250_001957"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBH0067893.1"
                     /translation="MSFKNDFLWGGATAANQCEGGYNEDERGLANVDVCPTGKDRTAV
                     ITGKLKMFDFDDEHYYPAKTGIDMYHNYKEDIKLFAEMGFKVYRMSIAWSRIFPKGDE
                     ETPNEKGLQFYENIFKECKKYKIEPLVTITHFDCPMHLVKKYGAWRNRKMIGFYEKLC
                     NVIFRRYKGLVKYWLTFNEINMILHAPFMGAGICFEEGEDVEKIKYQAAHHELVASAI
                     ATKIAHEVDSNNLIGCMFAAGSVYPYSCNPNDVWEATKLDRENYFFVDVQSKGKYPNY
                     ALKYMEQKGITPEMEPGDIELLNKYTVDFISFSYYNSRCVRTDENADDMAEGNIFTSA
                     KNPYLSYSQWGWPIDPLGLRITLNHVYDRYEKPLFIVENGLGAKDIADENGYVEDDYR
                     IDYLREHIKAMHDAVSIDGVDLLGYTTWAPIDLVSAGTGEMEKRYGFIYVDRDNSGNG
                     SLRRMKKKSFEWYKKVIASNGEDLY"
     gene            27795..28571
                     /locus_tag="KR250_001958"
     CDS             27795..28571
                     /locus_tag="KR250_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBH0067894.1"
                     /translation="MKYQHIIEVDKELCIGCGLCKNDCPVNNIIIENKKSVIKKQDCL
                     MCGHCAAICPTKAITLTGFDEPPIELTNKPKLDSDELLMAIKSRRSIRKFKDKEVSSE
                     IIKQIIEAGRYTPSAKNSQDVSYIVLDNKKSIYENEAVKFFRKIKPIANIAIKYSKEV
                     EIDDNFFFKHAPIAIMIITKDKISGSLAASNMALMAESYGLGVLFSGYFSDVANNSPK
                     LKKLLGLKRSNHVLTTLVIGYPDVKYRRTAQKEVATVRYL"
     gene            28587..28952
                     /locus_tag="KR250_001959"
     CDS             28587..28952
                     /locus_tag="KR250_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBH0067895.1"
                     /translation="MEKECQQRMQEITSKFKECRKAISAMGDETRQLILIALLESDFN
                     GIRVGEITKKTHLSRPAVSHHLKILKEAEIVNLRREGTKNYYYLDSKKSQWKSLTELI
                     NLIFVGIQHISENDIRKGD"
     gene            complement(29118..29867)
                     /locus_tag="KR250_001960"
     CDS             complement(29118..29867)
                     /locus_tag="KR250_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440038.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HBH0067896.1"
                     /translation="MILYFSGTGNSRYVARKIAQELNDELISLNQLIKEEKTDELISA
                     DKPFIFVCPTYAWRLPIVVTDFIKETKFLGSNRVYFVMTCGGDTAKSINYIQKLCKYK
                     EWQLKGMAEIKMPENYIALFSTTDKETSKQMIKEADKQIYRIISDIRNENEFETITPS
                     GLGGTIKSGIINTVFYKTIISAKGFHYTDKCIGCGKCVELCPLNNINLKNKKPVWKNN
                     CTHCMACICGCPTEAIEYKNKTQNRERYYLE"
     gene            30325..30750
                     /locus_tag="KR250_001961"
     CDS             30325..30750
                     /locus_tag="KR250_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0067897.1"
                     /translation="MINENLKSLRKINQYTQEELAEKLNVSRQSIAKWESGESIPDIC
                     SCIKLAKLYNVKLDDLVNHSEEKTGIIVPPKGKFFFGAVVVGERGQIVIPKEAREVFN
                     INAGDKLLVLGDEERGLGIVHQRDLINFIGHVGVVRKEE"
     gene            30782..31969
                     /locus_tag="KR250_001962"
     CDS             30782..31969
                     /locus_tag="KR250_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HBH0067898.1"
                     /translation="MSKIVFFSIPAYGHTNPTIEVVRELVDRGNEVLYYSFNEFKDKI
                     EGAGAKFICCDKYLPELLPGDEKKIGKDFPGLIEMIVDTTISLDEKVCRELKDFNPDC
                     IVSDSLSFWGKLFAQKLNIPYVCSTTTFAFNKHTAKLMKQNFIEIIRMIFGVRRINKK
                     IKLLQRKGYEVKNFISIVSNDNDTDTIVYTSKEFQPMVETFSSKYSFVGPSVSKLIIE
                     PKERKRKLIYISLGTINNKNVSFYKNCISAFKDSDVDVIMSVGRSTDIKSLGNIPNNF
                     EVKNSVEQIAILQKTDVFVTHSGMNSVNESLYYGVPMVLFPQHSEQRMVAKRVVHLGA
                     GIMLKEDKSESIKKAVFQVINDNEYKENATKLSKSFYNAGGSKKAADVILQIICNSRQ
                     VDI"
     gene            32161..32565
                     /locus_tag="KR250_001963"
     CDS             32161..32565
                     /locus_tag="KR250_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3139 domain-containing protein"
                     /protein_id="HBH0067899.1"
                     /translation="MKKYKVIFIVLIIGIISVTGFVVYKNCIKPWKDAETYIDKYMVK
                     QDISKDNIKSITKEKAKKASYEGILYKVYYKDDPRYEYQYFYSDNYYALYVNKVMLQI
                     YDMENNNKLLRSNEELKSVKYPLIYLKYKPDK"
     gene            33262..34122
                     /locus_tag="KR250_001964"
     CDS             33262..34122
                     /locus_tag="KR250_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBH0067900.1"
                     /translation="MKIIQILNNNVALVKRGKNEIIVFVKGIGFKKRVGQVINENEIE
                     RTYILDSYDMLEHFSYLLQNSDSNNIVLINEIIEYGEKALNIKASDYLSLTLLDHLEF
                     LLKRCEKNQFIKSPLVWNVKRFYPKHFEIGMHALKLIGDSKGINLPDDEAVSIALHFV
                     NMEVNKESHDSTIVELRTLADIVSIIKYHFNVELDETSTNYMRFTTHLQYFIQRIMSD
                     EASIDEDNSIDLYKQVSQLYPKSFGSVQKIRKYIKSQFNVDISISEETYLMLHINRVT
                     QRLEAKSDEI"
     gene            34112..35983
                     /locus_tag="KR250_001965"
     CDS             34112..35983
                     /locus_tag="KR250_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBH0067901.1"
                     /translation="MKYKDLNENIIKLIGGKENIKSVAHCVTRLRFTLNDRSIAQTEE
                     IKKLKGVIDVVSNDVAYQIIIGTHVVDVYNEFMSMIGLSSSESSIKSERKKGIRGIKG
                     IFNSIFVVVSETMTSVIEVLLAAGILASILALLNMSGILQSDSPTYQILDTLRSATFH
                     FLPVLIAIASAKRLNVNPYIAVVLAVTLLSSSIDGVEGLSLFGINLQATTYANSFIPI
                     LLGVWAMGIIIEGLKKILPKALHYFLVPVLSLIITLSVTLTVFGPIGMWIGDGLNYAC
                     MFLIDTLGNWSVVALYAALQPLLIVFGAANFTMPISLNSVTTLGYDPIFMGAATISDI
                     AVCGAMFGYFLKTRNKEQKQLFGTVSFSALMGVTEPSVFGVFMKYRRPFVAVMIGGGI
                     GGLIAGLAQVKTYGMVWGLAALPTYLVGGEVSNFTFMIISVVVSFVAATAASYLLGIP
                     SDEKEEVKGDNEKELELIINENNSRTISIGNVAKGQVIALGEVNDKAFSSGALGKGVG
                     VIPSESKSTVISPVDGTITIVFPTKHAYGIVTDEGLEILIHIGIDTVNLDGKFFESLV
                     TQNQRVKKGDALAVFESDKIIEEGFDSTIITVVTNTSDYLDVISSNDESEELLTVIV"
     gene            36003..37457
                     /locus_tag="KR250_001966"
     CDS             36003..37457
                     /locus_tag="KR250_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HBH0067902.1"
                     /translation="MNIKDTFFWGGSIAAHQCEGSWDSDNKGPAIMDFVTKGSYETPR
                     VITDKIEEKLDYPSHNGIDFYNRYKEDIALFKEMGFSALRISIDWSRIFPNGDDENPN
                     ELGIKYYEGLIDTLIENNIEPIVTLYHFELPMNLVHKYGSWNNRKLIDLYLKYSETVI
                     RRFDDKVKYWVTFNEMNHIDPQTEHSDIFTYMIAGIKYSEIENKVQTLANVGYNMTLA
                     GVKVAKLAREINPNNKIGCVFGLNPIYPYNCNPSNVLNAFLDNDRDYYQIDAMCNGKF
                     PQYKLKEYEKNNITIEVTDEDKKAFKEGKLDFIGLNYYFSSVSTPKENLSGDKSLFGG
                     IQNPYLEQSKWGWAIDPIGLRFTLNYIYRKYQLPILITENGLGAYDKIEEDGTINDDY
                     RIEYLSKHLSQMKSAIEEDNVECFGYLMWGPIDLVSATTGQMSKRYGFIYVDLDDEGK
                     GSFKRYKKKSFDWYKEVIKSNGEVLDFSTDIVVG"
BASE COUNT        15086 a         3898 c         6667 g        12440 t
ORIGIN      
        1 ataatattca agggaggtag ctgaaaatat atcaactaaa atggatatac tactatgtaa
       61 tttaatggat aacttaacta tagaaaaaga ctatctataa atatttgaaa taaatcttag
      121 tataaattat tagcagttta ctaatgtttt tttgtaaaaa atcaatcaaa aagttatata
      181 ataactttat ataacaaata gaaagaggtg aaactacatg gctaaaattg acaatatgat
      241 gtctatactg tggatgttaa actctgacaa aaaaattaca gcaaaacaga tagctgaaaa
      301 attagaaata aatataagaa cagtgtaccg atatattgat gtactttctg cgagtggagt
      361 acctataatt tcagatacag gtcataatgg tgggtatagc cttttaaata attttactaa
      421 agcacctctt ttttttgata tagaagagca aaaatctcta cttcatgcag ctctatttgc
      481 taaaaaagaa ggatacttct tagacgaagc attagataaa tcgatattaa agttaaaaat
      541 gtattcaaat caaaaccaag aagatatttt aacaaaccac ttaagagccc ttgaagtaat
      601 aaaaccaatt gggaaagtgt ctatagaatc agtacttaaa aaattggaga atagtatatt
      661 aaaggagcta tctgtagaaa tcaactatca tgcaggtcgt aaagcaaaat caaaatatag
      721 aattataaat ccatatggaa ttgttcactg gaataataat tggtacgtag ttgcattttg
      781 taatatgaga aatgaggtac gtagtttcag agtggatagg attagtgaaa tcatagaaac
      841 atcaaataca tttaacagac cgacagaatt ttcagcaagt gaatttttta taaaaggaat
      901 tatacctgaa ataaaagatg aacaaaatat aattcaatta gttgtagaag ggagtataga
      961 ttctttagat agtttaagcc aacattggtt tttagggcac tacttgaaag aaagaacacc
     1021 taataaaata atatttatgc tcgaagagga agtaatatgc aagtacatac caaatatact
     1081 tttaccttat agaaagacaa tacaagtaat tgaaccacta agccttagga agaaaataat
     1141 tgaagattta ctagaattaa ttgattatta tcaaatttaa tagcttaact gactgaatat
     1201 gtcagttaag ttattttata attagatata taaattaatt acataaggag taaaaaagat
     1261 gaaaaataga aaagtatata tgtatgtatt tgatactatg tcagattggg aagtggcata
     1321 tttaactgct gaattaaata caggaagata ttttaaaaaa ggattaaagc cactaaaagt
     1381 aattactgta ggaattgata aaaatcctat tataacaatg ggtgggttaa aagttttacc
     1441 tgaaattaca gttgatgagt ttaatatgga aagtaaggat ttattaattt tgccaggggg
     1501 aaatacttgg ttatccatga atcatgagat aatattagaa aaagttaaag aagctttaat
     1561 acaagattct atagttgcag ccatatgtgg agctacatta ggcctagcta aaaaagggct
     1621 attagatgtt agaaatcata caagtaatga cttagagttt cttaaaatgg taataccaaa
     1681 ttactctgga gaaaaatatt ataaaacgga atctgtagtt agtgatgaaa atttagttac
     1741 ggcttcagga atcgctccgt tggaatttac gtttcaagta ttgaaacttt tagatgtttt
     1801 tgaatcagaa gcattaaatt catggctaaa tctttacaaa actcatgact caaaatattt
     1861 ttttgaatta atgaattgta acaaataaat ttaaaattta tttttcaatt taatatggta
     1921 tagcattgtg atataaagaa caaaaaatta caatctggtg gaatagagta gtggtttcta
     1981 atatagattt ctttttggtg aatttaaatt ttatttaaat ttaaattcta aaccactttc
     2041 tatatagcca acagaaaagt gttaaaaaat tatcatatat ataattacta cttatagtaa
     2101 cacaaataga gctaataact cctataataa aatttatcat tagttacata tagaataaac
     2161 ctcagttttg attactgtag gaactaggat tatatgaagc taccactcat agatttcagt
     2221 tttttctgtt gtatcatagc atagaatagt aaattcatct gaaactttat tttaaattta
     2281 gaatttgcat atagataaat tatgggcatt attctgatga ttcgtctcca ataattaaaa
     2341 aaagaatgat aaaatacttg aaaatactag tctttattga ctagtgtttt tttatttcag
     2401 tgataaaata taccaattat acttcagtta caggaaatga tgtttttgag ggtacattgg
     2461 gaggcattta ggttgtataa ttataaattt caatgttata tgctcattag aataatagtt
     2521 agtaaatgat atgattgaga tgatagatga aataaactaa tttagtactg tattgtaatg
     2581 tttattataa aatcttttat aaaatatatt tatgaagata ttattgaata ttttcaatat
     2641 tctattgcaa tttatttgga aaagttatat atttataaca taaggtgtta taaatatata
     2701 acttttaagg ggtgatttta tatgtctaaa tcagagcttg gtgttaaggc aaatcttgtt
     2761 tcagagttaa tgatgctatc ctattttttt atagacttgt tattacaaaa gtttattttt
     2821 aaaggagaag gtatttggtt tacttttata attttattag ctttttgggt gtgcaatctc
     2881 atgtataata aaaaatataa tgatttagtt gatgaggcat caaaacttat gttgttaaag
     2941 gttgatcaga ttgcatcaaa aataatgtta tactctatgt ttgtaatagc tgttctcttt
     3001 tcagttaatc atacaagagc tttggacata tcaaatttgg atataggtat aatgctctta
     3061 gcagttttat tcatacaatc tttgtcaaaa ttaattttgt atatatattt tgataaaaag
     3121 ggaatatgta actaagtaat aatatttatg aaaacaaaaa ttaaaaaata tagagaatat
     3181 ataactttta aggagtgatt ttatatgtct aaatcagaaa ctggtgtaag atcaaagctc
     3241 atcttggaat tacttgtaat aacttatttt tttatagctg tattagctac taaatatatt
     3301 tttaaaggtc caagcactgc ttctcttcca gggttgagtt ggttaatagt ttttggatgt
     3361 tattcaattt ataataaaaa atataatgat ttagttgatg aatcatctga atttatacta
     3421 tcaaaggtta ataagattgc aataaaaata atgatatatt ctatagtaat aatatctatt
     3481 ttctttgcaa caccttatac aagtaatata aatatatcaa acctagatac aggtataatc
     3541 ctcattgcag ttttatttat acagtcttta tctaaattag tgttgtatat atactttgat
     3601 aaaaggggga tatgtaacta agtggcaata ttgatgaaaa caaaaattaa agaatacaga
     3661 gaaaaactcc ttatgacgca aaatgaactt gctaaattgg ttggggtaag acgtgagact
     3721 attgtacact tagaaaatgg gaaatataat ccttctttga agttagctat ggatatcgct
     3781 aaagtattcg atactacagt agaaaatttg tttgaattta ttgaagaaga atagtggaaa
     3841 gtttataaac atttataaga tggataatga aatatagtta tttaattatt aagtttatta
     3901 aaaatattta tataatatgt gattaaggtt agattataat tgttttaaga taataagaag
     3961 agtattataa atgataaaaa atagtgaata tacagttaat taataaacaa tagataaaaa
     4021 tttaaagttt aaatgtaaag aggatgtata attttatgaa atctaataag acactgttat
     4081 aatcgctatg taaggaaatc atattagaat tttctaagaa aacagttaag aatttggaag
     4141 taagtatgtg ttatttatat acttctgaaa catataaact aataagtgag gttgtatcag
     4201 atatgtcttg taagggtaat ttcttcaggt tcatcaacta tcataactct acagagggta
     4261 taagatatgc tttgtaaggg taatatatgc ttgattcaag aattagtgtt ataaaatgga
     4321 ttagcatacc aaaaggtcta ttaaagatat acagttaaaa agctatatag taagttaggt
     4381 aaggttaata atccaaacaa tgatttgatg attaaactgt ttagagcctt agatacaaca
     4441 gtaaaagaat tattttttaa taaggagaag taaaagataa aagaaaatca aaacttaggc
     4501 agttattgta gttacaaaat aaaggatttc cttatatatg gataaataat cgagtatata
     4561 taataaaaaa tatatgacct taattttgtt atgaaataac ttcaaaactt attttagcat
     4621 gtttagatta tataaatcat ataaaaaaag tagaatgagt gaatacttat gacaataatc
     4681 tagtagagat atacatgaaa ttatatattg actaagtaca gttattattt tataatgaaa
     4741 ataaagttat catacagata aataaacgga tcgttactga gatagatgta atcaggcgtg
     4801 acctaaaaat tctagtaaat gcttaaagta ttattagtct tttttaggtc tctttttgtt
     4861 tacagtggag gtagtttatg gttataaaga aagtatttaa taataatgtt gtatttgcca
     4921 gagataattc aggacaagaa gtaatattaa tgggaaaagg aattggattt aataaattta
     4981 ctaaagattt agttgaaata gataaaattg aaaagaaatt tatattcaat aatgaggaat
     5041 cattaagtga attacattca ctactagata ggataccttt aaaagatgtt gagcttgtaa
     5101 gtgaaattat tcaaatggga agagaagaat tgggtgataa tcttaatgat agtatattaa
     5161 tcacattatc agaacatata agttatatgt taaaaagaat ggatgaagga ttagtatttc
     5221 aaagcccgct tcaatgggaa ataagacaaa tatatcctaa tgaatataat tttagtaaga
     5281 aagctgtaga atatttaaga aaaaaattag ataaaaatat tcctgattct gaaatagcat
     5341 ttattacgat acactttgca aatgctcagt tagaaacaaa agatatgcaa gaaactttat
     5401 tgttaactaa gataattgat aatgtattag atatcataaa gtatcactat ggaatcgaaa
     5461 taaatgaata ttcctttgac tacacaaggt ttataagtca tttaagatgt tttataaaaa
     5521 ggcaattaag tggtgagaat acaaatgtag acacatcatt gttagatata ataaaaatga
     5581 aatataaaag tgattacaat tgtgcaaaaa aagtaaaaaa ctttcttgaa aagacgtata
     5641 aatggaaaat tagcgaaaat gaattgttgt acttaacatt gcatttaaat agattatcaa
     5701 atataaagta aatagtgtag gattgttact gtttggcagg catgacctaa gttggtataa
     5761 ttagttttta atatctaatt gtattgattt aggtctattt ttatacataa aattatgaaa
     5821 atggaggata gattgtatga aatttgaaag agagtctgaa gaaattataa aatatgtcgg
     5881 aggaaaagaa aatattatta gtttagttca ttgtgcaact aggctacgtt ttaagctgaa
     5941 agatacttca aaaactaaca aagaagagtt attaaaaatg aaggatgtac tatctgtagt
     6001 aaatagtgga ggacaatacc aagtggtaat tggaaataaa gtaactgatt actttgaaac
     6061 tattataaaa aaagcagttt tacaaggaca aaatactgaa gaacagggag aaaaagtttc
     6121 aatagtttct aaaatatttg aatcaatttc aggtgcattt tctccattaa tacctgcact
     6181 agctggagca ggaatgatta aggcactgct tacaatacta actactatga aatggatgtc
     6241 tgatacaagt agcacttatg ctatattatc agcagctgga aatgctgtat tttacttttt
     6301 accaatattc ttaggtatta ccttagctat aaaacttagt gcaaatccat atgttggtgg
     6361 agtgatagga gcagctttgt tagatcctaa ttttacagct ttaattggag caaaaggtgg
     6421 tgctaacttt tttgggatgc cagtaacagc aattgattat tcttttggtg tattccctat
     6481 atttgttgct atatttattt attactatgt tgataagctt ttaaagaaga ttatattaaa
     6541 ggatttacaa ttattcttag taccgatgat atcattatta gtcatggttc ctttaactgt
     6601 gattttattt ggaccaatag caactaatgt tggtgatata atatctaata tttttatggg
     6661 attatttgaa tttaacagag taattgcagg agctgcaata ggtatagcta tgccattctt
     6721 agcagtatta ggactacact ggggcttcac accaattact ttgcaaaact tggctacaat
     6781 gggtggagac ccaatagaag gtgcagctgt tgcagcagta tttgctcaaa taggtattgc
     6841 aataggttta tttatgaagg ttagaaaatc taaaaaaaat tcagaattaa aggtattagc
     6901 aggttcaaca gcagttacag gaattttagc aggagtaaca gaacctataa tttatggaat
     6961 tatattaaga tacagaagaa tgattccagt attggctatt gcaggaggta ttggtggtgg
     7021 tatatgtggt gtatttggtg ttacttgtaa tgcatatgta tttcataata tattctcaat
     7081 gccagtttat acaccattcc taggatatat attaggaata ggaacagcat tagtgctagg
     7141 agcagtattg tcatatttct ttggattgag taaagaagaa tgtgaagaag gtaatgaaga
     7201 agttgctttt gatgaagatg atggtataga ggagatatta tctccaatag aaggcttaca
     7261 aatagagtta tctgaagtag aagatgaagt gtttgcatca ggaactatag gtaatggcat
     7321 ggcaataata ccttcaaagg gagaagttgt gtctcctgta aatggaacaa taacaactat
     7381 ttttcctaca aagcatgcaa taggaattac ctctgataaa ggtgtagaaa tattaataca
     7441 cgtaggtctt aacactacta tgttagatgg aaagtattat actactcata tgaatgcagg
     7501 agataaagtt aaaaaaggag ataggattgt ttctttcgat aaggataaaa tagaaggtga
     7561 agggtatagt attgttacac caataatact aactaacaca aatgatttag cagatgtaat
     7621 agttaaagaa aataaacata ttaaaattgg aacgcctgta ataaaagttg tttattaatt
     7681 atactggagg agatatttct atgaaatcta atcaagaatt tttatgggga ggggcagttg
     7741 ctgcccatca agttgaaggt ggatataata aaggtggaaa aggaataagt attgcagatg
     7801 tgatgactgc aggtactcat actatttcaa gaaagataac agatggagta attgagggtt
     7861 taaattatcc taatcatgaa gccattaatt tttatgaaaa ttataaagaa gatattagat
     7921 tatttgctga aatgggatat aagtgtttta ggacaagtat tgcttggact agaatttttc
     7981 caaagggcga tgaatcaact ccaaatgaag atggattgaa attttatgat gatttatttg
     8041 atgaattatt aaaatacaat atagagccag taataacttt aagtcatttt gaaatgcctt
     8101 atcatttagt aaaaaactat ggtggatgga gaaatagaaa attaatagat ttctttgtaa
     8161 acttctgtga agtggtaatg aatagataca aggataaagt taagtactgg atgactttca
     8221 atgaaataaa taatcaaagt ataactacaa atcctattta tgcatttact aactcgggaa
     8281 taatctatga agagcaggaa gacaaagaag aagttatgta tcaagcagtt cattatgaat
     8341 ttgtagcaag tgcaaaggtt gtgaagatag gtcatgaaat aaaccctgag tttaaaattg
     8401 gatgtatggt ggcagctatg ccaagttatc cttattcttg caatccagag gatatgatta
     8461 aatttgtaga atctaataga gaacaactta tgtttactga tgtacatgta agaggtcatt
     8521 atccaagaca tacactaaaa ttatgggaaa gaaaaaatta taatttagat attacagagg
     8581 aagataaaaa aatcctaaaa gaaggaatag ttgattttat tggatgtagt tattatttaa
     8641 ctactgtagt aacagctgat aaaactatga aaacaacagg taatgactcg gcaggaaagg
     8701 ctgatgtagt tgaaaatcca tacttaaaaa tttctgattg gggatggaat atagaccctg
     8761 taggtctaag gttttacctt aatcaactat atgataagta tgaacttcct atattcattg
     8821 ttgaaaatgg atttggagca gaagatgtat taaaatcaga taatactgta gatgatgatt
     8881 atagaataga gtatttggct agtcatataa gggaaatgaa aaatgccatt gaaattgatg
     8941 gtgttgatgt tatagggtat actgtttggg gatgtataga tccagtatca tttacaacag
     9001 gagagatgaa aaaaagatat ggatttatat atgtagataa aaataatgat ggaagtggga
     9061 ctcttaaaag atataaaaag aaatcttttg attggtataa aaatgtaata aaatttaatg
     9121 gagaaatttt ataggtagat aaattttaat atttataaga ttatgttaag gaaaccatta
     9181 aaaattagat taaaatcgtg ggtatttaaa tccactaaaa gatattgact atttagtcaa
     9241 tatctttttt tattaaaaag taaatataaa gaggtaaggt taaaaattgg gatatatatg
     9301 atttatatag tatgatttaa ttagaaggag ctatattagt attcatttgt atagttggag
     9361 aataaatgtc agtcttacct tacgattttt ataatagtcc tatttaattt ctattgtaac
     9421 taatatgcaa gaatttaaga tagaagtatc tatgacttat ggtgtattaa taattggtat
     9481 ctgctatcta gtatcttttt aatttttttg attccaatat agtgctaact acgatacgaa
     9541 tatctttaaa tttatagcta aactatctaa ttgtttattg gaaagagtta agaaggctaa
     9601 agcataaaac acttaaaaag agaagatgta tatgagaaat ataattaact atgatgcaga
     9661 cttctaaaaa ttatttagtg aaaaggaact tagagtgtat aataaaagtt ccttttcact
     9721 aaggaaaacg attgttattt tctgatcttc attaccctga tattatttaa atataaacaa
     9781 caacaaaatt aacaagaagc aaattcaacc taaaatttaa aatatcggga ggtacaatat
     9841 gttaataaat atgaaggaaa tgctaaaagt ggcacaagaa aatcaatttg ctgtaccagc
     9901 atttaatata ggaagtgggc aaatacttaa agcagtagta cagagtgcta acgaaaaaaa
     9961 tgcaccagta atacttgcaa tacacccaaa tgagctaagt tttctaggag atagctttgt
    10021 agctagttgc atagaagaag caaataagag taaagtacct atggtaatac acttagatca
    10081 tggggaaaat aaagaacaaa tattaagggc aataagatgt ggatttacat cagtaatgat
    10141 tgatggctca catttaccat atgaagagaa tgtagctata tctagggaaa tagtagaaat
    10201 agcaaaagga ttaaatgtgt ccgtagaagg agaacttgga acaataggaa caacaggaac
    10261 atcaagtgaa ggtggtactg atgaaataat atatacagat tcaaaattag ctaaggattt
    10321 tgttgaaaaa acaggagtag aaacactagc tattgcaatt ggtacagcac atggtatata
    10381 tccaaaaggt tttaaaccag agcttaaact agatttatta aaagaaataa gagaagtagt
    10441 agatatacct ttagttcttc atggtggttc atcaaatcca gatgaagaaa tagcacaagc
    10501 agttaaacta ggagtttgta aaataaatat ttcgagtgat gttaagtcgg catattataa
    10561 gaagtgcaga gaactattag aacaaaaccc aagcttatat gagccagata ctatatatcc
    10621 accatgtata aaatctgcaa gagaagttat agagtttaaa atgaatctat tcaatgctat
    10681 agataagtta aagtgctttt ataaaaataa aaattaatta tgaggtaaca aattaaaagg
    10741 aggggagaat ttatgatagg taaaatacta cgaagcgatt gtattgtact agataaacaa
    10801 ttacaaacaa aaaaagatgt aattaattat atggcaaatc atttgtacca tggaaattat
    10861 ataaataata aagaaaagtt tatagctgat attaataaac gcgaaaaaga atcatcaact
    10921 aatatgggta atttaatagc tatacctcat gctaagtcaa atgtagtaaa tcaacctgta
    10981 atgatgtatt taagaacaga tagagagatt acagataatg atggtcaaaa acttaggtca
    11041 ttttttatga tagcagttcc agaatcaggt tcagatgaac atcttaagat tatatctaaa
    11101 ctagctacat atctaatgga agaaaatttt cttaagcatc tactaagtgc agaaactcca
    11161 tacgaaatat taagtatatt taataataaa gctgcagaat caggagattt tagtaataat
    11221 caaaataaag gtcttatagt tgggataaca ggatgtccaa caggtatagc tcatactttt
    11281 atggcaaaaa aagcattaga ggaagcaggt caagaacttg gatatgaagt taaaatagaa
    11341 actcatggag ctataggtgt agaaaataag ttgactaaag aagatataaa aagagctgaa
    11401 tacgtaataa tagcttgtga taaagaagtt caactgagta gatttatagg aaagaaagtg
    11461 ctacaaaaga aagtagtaga tgctaagaat gtttcattag ctaaaaattt aattataaac
    11521 gctgaaaatg gaatggctaa agaaattaaa ccaggtggaa agactttaga agattcaaaa
    11581 gaaatgcttc aagggtcaga gaaaaagggt gtttataagc acttaatgag tggtgtatct
    11641 gaaatgattc catttgtagt gataggtgga attggtatag ccatagcttt tatgtttggt
    11701 atatttgcat cagacccaac tcatgaaaca tacaacccta tagctggatt ctttggacaa
    11761 cttggaggcg attcagcatt taagttattt ataccgatac ttgctggttt tatagctaaa
    11821 tcaatagcag gaagacaagg atttgctcct gctatgatag ctggttttat ggcagttatt
    11881 ggaggttcag gttttatagg tggtatggta gctggttttg caacaggata tatagcttta
    11941 ggagtaatga agataacaga aaatttacca gatgtattaa aaggagtaaa tgctgtattg
    12001 atatgtccac ttttgattgc atttacgagt ggagctataa tgttctttgt agttaatacg
    12061 ccagcatcat ggttaaatga agctttacaa tcttggttac aaagtatgtc tggaactaat
    12121 aaagtattat taggtgcaat attagcaggg atgatggcat cagatatggg aggacctatt
    12181 aataaaacag cttcagcttt tggattggct atgttttcaa gtaatatatt tgaaccatca
    12241 gcagcattaa tggtaggagg aatggtacct ccattagcaa tatcattagc tatgttaata
    12301 ttcaaaaata agtttactaa agaagaaaga aagtcatctc taccaactgc tataatgggg
    12361 gcatctttca taacagaagg cgcaataccg tttgcagctg cagacccatt aagaatgatt
    12421 ccagcaaata taattggttc ttgtataggt ggggcaatat gtatgagttt agatataact
    12481 ttaatggcac cacatggggg aatatttgta ataccatttg catgtagtgc accagttgca
    12541 tatatagctt gtatattgat tggtacagta ataacagcat tgattatagg ggtaacaaag
    12601 cctactataa ggagtgaaaa tagtgaagtt gaattacttg gtgcttaaat tataaataat
    12661 agtaaaaata attaatggaa taacaacaag tatatagaat aataataagg atatttagaa
    12721 tcagttaact acttctaaat atccttaatt tttattttga aattatatta tctattatat
    12781 caatttagta aggtgtatca taaattcttt tatgatacag aagtatctta gtaattttac
    12841 atagttagta aatatgttaa aatagtactt aatataaaca tagaaatata taaacatagg
    12901 tagggagggc taagtatgct tgtgtcgagg caaattgaat ttcttaaatt gatactaaac
    12961 gaaaataaat atagacctat aaaatacttt aaagataaat taaatgtatc tgataaaact
    13021 ttacaaaaag atttgaagat gattgagaaa tatttagaaa cttttaatat aaaaattgat
    13081 ataaaaagag gctatggaat attaatagaa gatttagcaa agaagaatat agacttaatt
    13141 aatagtttaa atatacaatc gaaggaaaat aaatctttat cagtagagca gagaagaatg
    13201 gagattttaa agtatttatt actaaattca aataatataa cttcaataaa tcagttagca
    13261 gataaatatt atgtaagtaa aacttctata gtaaatgatt ttaaatatat agaggcatgg
    13321 ataaaagaat acaatttaaa acttaataaa actttagaag gaactaagat tataggtaaa
    13381 gaagtggata taaggaaatc gattgccaaa atgatggatg atttattaga tgaaaattat
    13441 gaacaacatg atatatctga gttaacaaga ttagattctg ctacatttag tgcattgata
    13501 aatttatttg atattgattc tattatattt gtagaaacta taattacaga attagaatac
    13561 aatttaggat atactataaa tcagccctac tatataaatt taattaccca tatacttata
    13621 tgtttaaaga gaatcgaaga aggtaatcaa atagaatctc aggaagaaag agaaatttct
    13681 gtagataatt tggacgagtt agtatataaa aatgtcagta cacttataga taaaatagaa
    13741 aatagataca gagtaaaaat aacgaaagat gaaattatgt acatatatat ttttttggta
    13801 tcatctggat ttagtagcga aaataatagt agttacaatg atgaatcaga tagattaagt
    13861 gaaagtgaaa aagtttcatc tataatgata gagaatatgt cacgattttt aaatataaat
    13921 ctcaaaaatg atgacttact acagaaaagt ctagcatctc atgtaaggcc tatgttaaac
    13981 agattaagat atgatatcca aataaaaaac ccgttgttag gagagataca agagcgattt
    14041 tcagatgtgc tgggattatg tatgatgact ataaatatta tgacagagta tgataatctg
    14101 aagaatatta gtatagatga agtttcgtat cttgccacat attttcaagc ggcaatagaa
    14161 agaaatatga gtagtaaaag agtaatagta gtttgtcata caggatatgg aacatctcag
    14221 ctccttgcag ccagattaaa aagagaattt cctgaatgga ctatagttga tatagttcct
    14281 atgcatcagt tgagtaagcg aaatttagat gatgtagact ttgtaatatc tactgtaaac
    14341 ttagatttga aagataaatt acatatagtt gtatcagtac tattaatgga aaatgatatt
    14401 aataatataa aaaatgcttt actttctaaa cctaaaaaaa gtatgaactt agatacaaac
    14461 ttattaggaa tgtatttaga agataatata tattttaatt ttgatgtagc tcagatggaa
    14521 agtttaatag gtgtgaattt agggagtaaa tatgaatcta tttcattagg aaaagattta
    14581 aatatatata tacataagca aagtaaatta aataaaggga taatgaatat caataatgtt
    14641 actagaacga ttgatattta tttagaaatt agtaatcaaa gttttttaaa gaatatttta
    14701 atggaaatat caaatttata taggcaaaag aactatataa actatataat agattgcaag
    14761 agcaaagagg atattaaaat attatttaag aataatctat tggagaaaac aaatggaaat
    14821 tagagaaata ttaacagaag aaagcattat tttagatttg aaatcaacta caaaagaagg
    14881 cataatacag gaattagtag ataaattata cgatacagga agtataactt taaaaagtaa
    14941 gtttataaag gcattaaaac atagagaaaa aataggctct actagcatag gttatcaaat
    15001 ggccatgcca catggaaaaa caaaatatgt aaagaaggca tccatagtaa taggtaaatc
    15061 taaaaaaggt gtagattggc caagtataga tggcttacct gtaaaggtaa taattatgtt
    15121 tgctatgcca caatctggag gcaatgaaga tatgatatta atatctaaaa tagcttacat
    15181 tttaggtggt aaaaatatag gtgaaagact tttaaatgca aatacaaaaa aagaaatatt
    15241 agatatattt tatgaagtgt aggtattgta taaattataa atttgaggtt tttaaaataa
    15301 aatgtgtatt cttttaaata gtataaatta atttttagac actagagagg agaggatata
    15361 tatggcaata tatttagaaa tgcctaaatt aatacatgaa tttccttttc gtagtctatc
    15421 aaatgaagga gaagtattaa caacaccaca ctggcataag gaaatagaaa tattacttat
    15481 aacagaaggt gtagtaaatc tatttataaa tgacaagcct atgcaattaa aaaaaggcga
    15541 gatagtgata ataaaaggag gagatattca ttatatactg ccatcaccgg gaagtgaaag
    15601 attagtattt caatttgata tatctttttt taatgattta attttattaa atgaggaaaa
    15661 agaaagctta attaatttat tcagtagtat aaaaaaatgt agtagagatt ggtcagcaaa
    15721 aataagaaag tcagttttcg atatattgat agatatctac aatgaagacc tatacaaaat
    15781 agaaggatat agttatgcaa taaaaggtaa aatgtataat ttaattccta tattatatag
    15841 acagattcct agagaaattg aagaaaatac agtagaatat gaaataaact ctaaagagat
    15901 attagagaga ttgaacaata tattcaaata tgtagaaaaa aattataaac aacctataaa
    15961 attagaagat gtgtcttatg aagtaggatt tagtgtatat tattttacta aatttttcaa
    16021 aaaaaataca ggaaaaacat ttataacttt tttaaatgaa tatagattag aaaaagcaaa
    16081 gtggatatta cttaataaca atatatccat tgtagatttg gttgaagaag ttggttttgg
    16141 aagtacaaaa actttttaca gagtatttaa agaaaaaatt ggagtctctc cattagaatt
    16201 taaaaaaaga tataatagct tggaaaaaaa agggagtgtc tcaaaatgaa cttagttcat
    16261 gaggcacttt cttttatatg aaatcaaata tattttcagt atttcaacaa tgaaaattcc
    16321 attcatttat aaagtctaaa ttttagttct caatacaacc tctatttcga aataaatcct
    16381 tttatatata aatatacagc aatatatgag aataagataa caataatgag gatataaaaa
    16441 tgtatttgct atatatataa ttatatacat agatatctaa tattgatatt taatatgaaa
    16501 gtattgaaaa tagacaatat taattatagt gagtaaaaaa tataattgga tttttaaatt
    16561 gaattattta aaaggtatag tatgaaaaaa atcttataac taaatatgag atagaaatta
    16621 taaaacttta tattgtgaaa gaggtagatg catgaaatta gggaaagatt ttttatgggg
    16681 aggggcaatt tcagccaatc aaagtgaagg attttattta gatggaggaa aaggtttatc
    16741 tcagtttgat aaactcccat taaatgataa gagattaaaa gatgtatgtt tagatgagga
    16801 taatatttta aatgaaaata atcaatacta tccaagtcat atagggatta aattttgtga
    16861 aaattacaaa gaagatatag caatgttagc agaaataggt tttaaatgtt ttagattttc
    16921 tatatcttgg tcaagaatat tcccaacagg agaagaaagt gaaccaaatg aaaagggatt
    16981 acaactttat gataatataa taaaagaatt aaaaaaatat aatatagagc cattaataac
    17041 gataagccac tttgatatac cttattctct agttgaaaaa tattctgggt gggctgatag
    17101 aagagtggtt aatttatatg taaaatatgc tgaaattttg atgaaaagat ataaagatga
    17161 tgtcaaatac tggattcctt ttaacgaaat gaatatgata atgcatattc catatatagg
    17221 tgctgggtta acttttatag ctgatgaaaa tcaacttcag aaaaaatatc aagcagcaca
    17281 ccatcaatta ttagctaatg caaaaactat agaaatagga aggtatataa ataaagaatt
    17341 taagtttggg tgtatgttag ctgctggaaa aacatatcca tacacatgta aaccagaaga
    17401 tgtatttgca actttagagc atgaaaggca taatttgttt ttttctgatg ttcaagttag
    17461 gggtgtgtat ccaagcttta tgaattatta tttaaaatca aatcaaataa aattaataat
    17521 aaaagaggaa gatttatcaa cacttaagga taatactgta gattttgtat cttttagcta
    17581 ttattctagt gcttgtagtt cagctagagc tgaaggttta gaaaaatcaa aggcaaacgg
    17641 accagaaacc ataaaaaatc catatcttcc aaaatcaaat agtgtatggc aaaacgaccc
    17701 tgtagggtta agaataacta tgaatcagtt atatgaaagg tatcagttac ctttatttat
    17761 agtagaaaat ggactgggta ctcaagatat tgtaaatgaa gatggaaaaa taattgatga
    17821 ttatcgtata gattacatga aagaacatat taaaaatatg attattggag taaatgaaga
    17881 tggaatagag cttataggat atcttatgtg gggatgtata gatgtaacca gtgtaagtga
    17941 aggaaagatg acaaaacgat atggcataat tcatgttgat tctgatgact atggaagagg
    18001 ctcatttaaa agaactaaaa aacaaagtgc tttttggtat aaaaaagtaa tagaaacaga
    18061 tggagaaatt ttatttaatg acatggaaac tcatgcataa ttcattttaa gaaaggagtg
    18121 ttattatgag tttaatattt actactaagg accaacttat agagcaaggg tttagtaagg
    18181 atacaataaa atccaatgaa acgctgttta cactagctaa tggacattta ggagttagag
    18241 ggaatattga agagtctgat tttaataatg agtatgtaga taatatgggt acatatgtaa
    18301 atggatttta tgaagaatct ccaattacat atggagaaag cgcttatggc tatgcaaaac
    18361 aaaatcagac tatctgtaag ttacctaatg ctcatatagt aaatttctct attgaaggag
    18421 attgctttga tttatcaaaa ggtattacaa gtgaacattc taggatacta gatttaaatg
    18481 aagggattct taaacgttca tttatatggg aaaactctcg tggtagaaaa gttaaagtat
    18541 ctatagaaag attagtatca caagatatag aagaattaat gatgatttca tatacaataa
    18601 caccattgaa ttttaatgga aaaataaaat ttaaaaataa aatggaaaat agtatggaga
    18661 agtttaattg tgaaaaagta gaggacccaa gagtggcatc tataaataag aagcaattta
    18721 aaattaatgt aaaaaaatat aacaataagt attttatgaa tttagttaca gaaagaagta
    18781 gattagggtt atggtgtggg tttgattcat atattacttg tggaaatatt acaaatgaga
    18841 cctataatgt ttcagatgaa tgtattgagc gagaaataga agtctatgcc aaaaaagatg
    18901 aaaggttaaa tttagaaatt gttgtaggct atagtagagt atattctgat ttaaatctaa
    18961 atgaagaacg tttaaaatca ttagaatgtc tggagtcgtt aaaatgttta gaatcagaag
    19021 cacatttaaa atcattagaa ttggttttaa gagaagcatg taatattgga tttgataaag
    19081 tcaaaaatat tcagagaaag aaaatgaatg agttttggac aacctctgat gtagagattg
    19141 aaggcgataa aactcttcaa ctggggataa aaaccaattt atttcatatt tatcaatcag
    19201 taggaagaga tggaaagact aatatatcag caaaaggtct tacaggtgat ggctatgagg
    19261 ggcattattt ctgggataca gaaatgtata tattaccatt ttttatatat actcaaccat
    19321 ccattgccag agctttatta gattatagat actcaatctt agatgcatca agagaaagag
    19381 ctagagaact tggaataaat aaaggttgct tatattcatg gagaactata aatggtaatg
    19441 aatgcagtgc atattatcca gcaggaacag cacagtttca tataaatgca gatatagctt
    19501 atggtattag aacgtatttt gaagcaacta atgatgaaga atttatgatg caaaatggct
    19561 tagaaattct tatagaaaca gcaagatttt gggttgaatt tggagattat atagaatcaa
    19621 aaaataataa attctgtata aatggagtaa caggaccaga tgaatataca gcaatagtaa
    19681 acaataatta ctacactaat ttaatggcta agtataattt aatatatgca gtacagatgg
    19741 tagataaatt taagcataat ttattttcaa atgaagtatt tagaaaagtt aaattacaag
    19801 aatatgaagt gaaaaactgg ataaatgccg ctcaaaatat gtatttacca tatgatgaag
    19861 agaagaaaat aagcaaacaa gacgattcat ttttagaaaa atcagtatgg gattttaaaa
    19921 atacaccaaa agaaaaatat ccattacttc ttcattatca tccattaaca atatatagat
    19981 atcaagtaaa taaacaagca gatactgtac tttcagcgtt gcttttccct gatgagttta
    20041 gcttagaaca aaagaagaga gattttgaat actatgaaaa aataactact catgattcat
    20101 ctttgtcaag gtcaatattt ggaatgatgg caagtgaaat tggaaatcat gaaaaagctt
    20161 ataactattt tatggacaca gcattaatgg atattaataa cctacaaaaa aatacaagtg
    20221 atggaataca tgctgctaat atgggtggaa catggatgag tttagtatat ggatttgcag
    20281 gaatgaaaat aaaaaatgat aaattaagtt ttgaaccaag gatacctaaa cactggaaaa
    20341 atattaaatt taatatatta tttagagata atttagtttc tgtaaatata gaagaatata
    20401 aatcttatta taagttaatc aaaggtagaa atataaccat attacatgat aataaagaaa
    20461 taaaattatc aaagaacata atagaagtta ataattgttt taaagggagt aattaatatg
    20521 aaagcagtct tatttgatct tgatggagtt ataacagata ctgctaagta tcattttgaa
    20581 gcttggcatg agatagcaaa atcgctagat atagaaatag atgaaacttt taatgaaaaa
    20641 ttaaaaggca taagcaggga agaatcatta gataaaatat tgttatctgc taataaagta
    20701 gatagttata ctaaagagga aaagttaaag gtgtgtgaag aaaaaaataa catttatata
    20761 aagttaataa actcattaac atataaagat atattaccag ggataaatga gcttataaaa
    20821 gaacttaaaa aaaataatgt gaaattggga atagcatctg ctagtaagaa tgcacctagg
    20881 atattagaat ctttgggaat aaaagatgaa tttgattgta tagtaaatcc atctttatta
    20941 aaaaggggaa aaccatatcc tgatattttt attgaaggga gcaagattct aaatttaaaa
    21001 caatatgatt gtgtaggaat agaagattct tattcaggta ttaaagctat aaatgctgct
    21061 aatatgacat caattggtgt ggggaaaaaa gaagtactag gaaaatgtga ccatattgta
    21121 gaagatacta gcttattaaa ttacaataca ctattacaag cttggagtaa aaagattaat
    21181 ttggattaaa attatattaa aaattatttt aaaagagagg ataaggtatg aagaataaat
    21241 atgaagaagt agctcaattg ataattgatg gacttggagg aaaggataat ataattgact
    21301 taactcattg tatgacacga ttaaggttca tcttaaaaga tgaaaagaaa gtcaatgcag
    21361 ataaattaaa atctatagat aaggtagttg gagtaaactc aacttcaact caatatcagg
    21421 tcataatagg taatgaggta ggtgttgtat ttaatgcaat aataaataag gggataaata
    21481 ccacaggaga tattaaaagc gatataaaaa ataaaaataa aaaaaatagt attttaaata
    21541 atataataga tacaataact ggttgtatga cacctatgat accagccctt acagcatctg
    21601 ggatgataaa agttatatta tctctttgca ccacttttaa ttggctatca tcaaattcaa
    21661 gtacatatag agtattagat ataataggag atgtagcatt ttactttatg cctataatac
    21721 ttgcaataaa tgcagctaaa aaatttaatg ttaatacatc aatagcagtt atagtagtag
    21781 gtgtgttttt acatccaaat ttctctgcat gggtatcaag tggggaccct atatcattta
    21841 taggagtacc aattcaagat gttatatatg cagcatctgt tatccctgca cttttaacag
    21901 tatggatgat gtcatatata gaaaaattca ttgataaatt aacacctagc atgttaaaaa
    21961 caattttaaa tccaacttta gtattattaa taagtgcgcc aatagcatta atagtaattg
    22021 gaccaatagg aaatttctta ggagagggat tagctagtat aataaattta ctacaaggaa
    22081 ggttaggatt tataatggtt tgtttattag cagctgcaat gccatttatc gttatgacag
    22141 ggatgcatca tgcattgact cctatattca tatcagcatt tgcaactaca ggacaagaat
    22201 cgctaatatt aatatctcaa gtttgtgcta atttagcgca aggtggcgca acattagctg
    22261 tagcattaag aagcaaagat agtaagatga aacagttagc atcagctgca tggatatcag
    22321 ctatcatggg tataactgaa ccagctcttt atggagttac attgaaactt aaaaaacctg
    22381 ctatgttagc atctatagga gcaggaatcg cagggttatt tgcaggaatt actcatgtga
    22441 ctttatatgt tccacaaaat agtataatgg cagtacttgg attatcaggg gaaaagggga
    22501 tttcaaatat agtaaatgga cttatagtta tatttatagc attggttgct ccatttatta
    22561 tgacatatgt ttttggagtt gaaaaaaatg ttaaataaag tacagataat gtagtagagg
    22621 aagtataata ttaactattt acattaatag aatttttatt attattgact ataacttgtt
    22681 ttgtatttat attgatgcaa agaaataagt taatattaat aagaataatt attagaactg
    22741 acaaaaagca acataaaaga ttgatgaagt atatggaaat aaaaagttga caaaatatgt
    22801 ctgatgtttt atcatattaa ttggaattag gtagtaattg tttaagtaca agctaagctt
    22861 attcattaat aatagcagta tcctaggaag gagtatgccc attattatgg ataagctttt
    22921 tttatgctta aaaggaggat aatcatgata attaataaga tattaaacaa taatgttgta
    22981 atcacattag atgataatga tgaagaagtt atcgttatgg gtaaaggaat tggttatcaa
    23041 aaatcaaaag gcaatctaat agataaaact aaagtaaata aagtatttag aatatcaaat
    23101 aaagaaatat caaacaaact acaagaatta ctcaataata ttcctataga acacatgaaa
    23161 ttatcaagtg aaattataga atacgcacaa ataaagttaa acaagaagct aaatgaaagt
    23221 atttacatat ccttaagtga ccatacatat tctgcaattc aaaggatgaa agaaggaata
    23281 aatgtaaaaa atgcaatcct ttgggaaatt aaaagatttt ataaagaaga gtttgaaatt
    23341 ggaatgaaag cactagatat tattgaaaac aaaacaggta taaaattacc agaagatgag
    23401 gctggattta tagcctttca tatagtgaat gctcagttaa gtgaaggtca tactttggca
    23461 tctgatatta cgaagttaat acaagaagta cttagcatag ttaggtatca ttttaggata
    23521 gaatttcatg aagaatcagt gttttattat agatttataa tgcatttaaa gttttttgcg
    23581 caaagattgc ttttagatag tgcacatgaa ggtgagacag acaaagaatt actaagcatt
    23641 attaaatcta aatataataa agagtttgaa tgtgtagtta aaattaaaaa tttcattaaa
    23701 aaacaatata actacatatt aacagatgat gagcttatat atttaacaat acatttagct
    23761 aaagtagtta aagactcaaa tatgtaagtg aaataatttt tcagtagtat attaagaatg
    23821 attgaaagga gtgatactaa caaaattata cttatggttg gatggtaaca ttgagttggc
    23881 caaaccttca aatttcaaaa aattaattta aacgaaatat ggaggagaaa aatgggaaaa
    23941 tacaaacaat tagcacaaga gatagtaaac aatgttggtg gaaaagaaaa tattagtggg
    24001 ttagtacact gtattacaag attacgtttt actttaaaag atgaaagtat agcaaatgac
    24061 aatgtattaa aaaacatgga aggtattgtt actgtaatga aaagtggagg acaatatcaa
    24121 gtagttatag gaaatcatgt tgaagctgtc tataaagatg tagttgaaat aattggttta
    24181 gatgattcaa gtacaagttc agaaactaaa aaatcaggta atatactaga taaaggtata
    24241 gatataatat caggaatttt tcagccagtg ttaggaataa tggcagcttg tggtatgttg
    24301 aaaggattta atgcattatt tgtagcaatg ggattatata gtgatgtatc tggtggttat
    24361 atggtaataa atgcagcagg agatgcatta tttacattcc ttcctttgtt cttaggatat
    24421 acttcagcta agaagtttgg attaaaacca atgttgggat tagcactagg agctgcagta
    24481 tgttatcctg ctatacaagg tagcagtata tcagcaggag cagatgtgat gtacacttta
    24541 tttaaaggaa caatgtttgc atcaccagta tatattgatt tctttggact accaattgta
    24601 tcaattgatt atactggaac agtcgtacca attatattta tagtgtattt tgcttctagg
    24661 tgtgaaaaat tatttaataa gtgtgttcca aatttagtta agtttttctt tgtgccaatg
    24721 cttacattat taataacagt gcctgttgca cttatagtta taggccctat agcaacattt
    24781 ggttctacac ttatttcaca aattgtaatt tcaataagag attttagtcc actattggca
    24841 ggtgctatag taggatttac ttggcaaatt cttgttattt ttggtatgca ctggggattt
    24901 attcctgtat atataaacaa tgttatgact ttaggatatg ataatgttat gatgccattc
    24961 tttgcctgta catttgcaag ttcggcagtt gtgttggcaa tatttttcaa gactaaagat
    25021 aaaaaactaa aagagatggc aataccaaac tttatatctg gtatatttgg agttacagag
    25081 ccagcaattt atggaatatt attgccttta aagaaaccat ttataataag ttgtattgct
    25141 agtggtattg gaggcgcttt ctatggattc tttaatctta gaaaatttat aactggagga
    25201 atgggaatat ttgaacttcc agcaatgatt gaaccagatg ggggaatggg taacttaata
    25261 gtagctcttt ctggtatagc tatatcaatg gtcgtagctt ttgtattaac tatgatttta
    25321 tataaagatg agaaagtaga agagtctaaa aaagtaagaa ataagggtaa agaagaaata
    25381 aaagaagtca agtctactaa attagaaaga gaaatagtaa caagtcctat aaaaggtgaa
    25441 gtattaaaat taagtgatat agaggatgca gcatttgcat ctggtgtact aggtcaaggg
    25501 gtggcaataa tacctagtga tggaaaagta gttgctccag tagatggggt agttacaacc
    25561 ttatttccta gtctacatgc tatagggatt ctttctgata caggtgttga agtgcttatt
    25621 catattggat taaatactat tcaattagag ggaagaggtt ttgaggcatg tataaaacaa
    25681 ggtgatagaa ttacaaaagg tcaaactata ttaaactttg atgtttatgc aataaaagag
    25741 ttggggtata gtactgtaac tccaattgtt attacaaatt catcacaatt tttagatgta
    25801 gtagaaacag aaagtaaaaa tattgaatta gaagacaatc taattacagt cttattttag
    25861 taaagattga ggtattagca taatctagtg ctttggttat aattttagga ggctttgact
    25921 atgagtttta agaatgattt tttatgggga ggagctactg cggccaatca atgtgaaggt
    25981 ggatacaatg aagacgaaag aggattggca aatgtagatg tatgtccaac aggaaaagat
    26041 agaacagcag ttattacagg gaaactaaaa atgtttgatt ttgatgatga gcattattat
    26101 ccagctaaaa ctggtattga tatgtatcat aattataaag aagatataaa gttatttgct
    26161 gaaatgggat ttaaagtata tagaatgagc attgcatgga gtcgaatatt tccaaaaggt
    26221 gatgaagaaa caccaaatga aaagggactt caattttatg aaaatatatt taaggagtgt
    26281 aaaaaatata aaatagagcc tttagtcact attacacact ttgattgtcc aatgcattta
    26341 gttaaaaagt atggtgcatg gcgtaatcga aaaatgatag gtttctatga gaaattatgc
    26401 aatgttatct ttagaagata taaaggatta gtgaagtact ggctaacttt caatgaaata
    26461 aatatgattt tacatgctcc atttatgggt gctggtattt gctttgaaga aggtgaagat
    26521 gttgaaaaga taaaatatca agctgcccat catgagcttg tagctagtgc aattgcaact
    26581 aaaatagcac atgaagttga ttcaaataac ttaattggat gtatgtttgc agctggaagt
    26641 gtatatcctt atagttgtaa tcctaatgat gtatgggaag ctacaaagtt agatagagaa
    26701 aattacttct ttgtggatgt acaatctaaa ggtaaatatc ctaactatgc gttaaagtat
    26761 atggagcaaa aaggtattac accagaaatg gaacctggtg atattgaatt attgaataaa
    26821 tatacagtag attttatttc attttcatat tacaatagcc gttgtgttag aaccgatgaa
    26881 aatgcagatg atatggctga aggaaacata tttacatctg ctaaaaatcc atatctttca
    26941 tacagtcaat ggggatggcc aattgaccca ttaggtttga gaattacact aaatcatgta
    27001 tatgatagat atgaaaagcc actatttatt gtagaaaatg gattaggtgc taaagatata
    27061 gctgatgaaa atggttatgt agaagatgat tacagaattg attatctaag agaacatata
    27121 aaagctatgc atgatgcagt ttctatagat ggggtcgatt tattaggtta tactacatgg
    27181 gcacctattg atttagtaag tgctggtaca ggtgaaatgg aaaagcgtta tggatttata
    27241 tatgtagata gagacaatag tggtaatgga agtctaagaa gaatgaaaaa gaaaagtttt
    27301 gaatggtata agaaagtaat tgcttcaaat ggtgaagatt tatattagac aagaaatcta
    27361 tgttaaataa gaaatctata ttagacaaga aatctatgtt aaataagaac tttgtattaa
    27421 ataagaagag tataaaaatt aataataagt ataattttta acttttttaa ctatagaaaa
    27481 tcaatataga attattttac attgtattga tgcaatagta tgattcacaa ataaaatttt
    27541 agaaaataaa attgttaaga agtatgggaa gtaagtgcaa tttactttgt aataaaagat
    27601 gaaaaaggca agcttaggtt ttaagcttgt cttttttatt atgtataaac tatgatattt
    27661 taaaattcta tgaaaaattt atttaataga gaaattaaaa atttaatggt aatcaaattc
    27721 tttaccaatg aatataaaaa atgtctacgt gttataatag tttaaactaa taaacgaaag
    27781 gtagatgaat gaatatgaaa taccaacata taatagaagt tgacaaagaa ctttgcattg
    27841 gatgtgggct ttgtaaaaat gattgtccag taaacaatat aattatagaa aataaaaagt
    27901 cagtaattaa aaaacaagat tgtctaatgt gtggacattg tgcagcaata tgtcccacaa
    27961 aagctattac cttgacagga tttgatgaac ctcctataga acttacaaat aagccaaagc
    28021 tagattcaga tgaattacta atggcaatta aatctagaag aagtatacgt aaatttaaag
    28081 ataaagaagt ttcttcagaa attataaagc aaattattga ggcaggtagg tataccccta
    28141 gtgctaaaaa ttcacaagat gtatcatata ttgttttaga caataaaaag tcaatttatg
    28201 aaaacgaagc agtgaaattt tttagaaaaa taaaacctat tgcaaatata gctattaaat
    28261 attcaaaaga agttgagatt gacgataact ttttctttaa gcatgctcct attgcaataa
    28321 tgataattac aaaagataag attagtggtt cacttgcagc atcaaatatg gcattaatgg
    28381 cagagtctta tggtctaggt gttttattta gtggatattt ttcagatgta gcaaataatt
    28441 cgccaaaatt aaaaaagctt ttaggtctaa aacgtagtaa tcatgttctt acaacattag
    28501 taattggata cccagatgtt aaatatagac gtactgcaca gaaagaagta gcaactgtta
    28561 gatacttata agagagggtg gattttatgg aaaaagaatg tcaacaaaga atgcaagaaa
    28621 taacgtctaa atttaaagaa tgtcgcaaag ctatctctgc tatgggtgat gagactagac
    28681 aattgatttt aattgctctt ttagagagtg attttaatgg tatacgagtg ggagaaatca
    28741 caaaaaaaac acatctttct cgtcctgctg tatctcatca tcttaagata ttaaaagaag
    28801 cagagattgt taatttgcgt agagaaggaa cgaaaaatta ttattatctt gattcaaaaa
    28861 aatcacaatg gaagtcacta acagagctaa taaatcttat atttgttgga atacaacata
    28921 ttagtgaaaa tgatataaga aaaggtgatt aaattatact tgatttaata caaaaagatt
    28981 gatttaggaa ctaaaaaatt cactttgaga cactatcttt tgaactatac ccattttagg
    29041 agacggttaa aatatgtaaa actgtataaa acaatgggta tagttcattt tataatggtt
    29101 taaatattaa gaatatatta ttctaaatag tatctttctc tgttttgagt tttattttta
    29161 tactcaatag cttcagtggg gcaaccacaa atacatgcca tacaatgagt acagttattt
    29221 ttccaaactg gttttttgtt ttttaaatta atattattta aaggacatag ctctacacat
    29281 tttccacagc caatgcattt atcagtataa tgaaatcctt tggcactaat aattgtctta
    29341 taaaaaactg tatttattat accactttta attgtacctc ctaagccact aggagtaata
    29401 gtttcaaatt cattttcatt gcgtatatca gaaataatcc tatatatttg tttatctgct
    29461 tctttaatca tttgttttga tgtttctttg tctgttgtag aaaataaagc tatataattc
    29521 tctggcattt taatttctgc catacctttt aattgccatt ccttgtattt acatagtttt
    29581 tgaatatagt ttatggattt tgctgtatct ccaccacaag tcattacaaa gtaaactctg
    29641 ttgcttccta gaaattttgt ttcttttata aaatccgtaa ctactattgg caatctccat
    29701 gcataagtag gacatacaaa tatgaatggt ttatctgctg atattaattc atctgttttt
    29761 tcctctttta taagttgatt tagtgatatc agttcatcat ttaattcttg tgcaatttta
    29821 cgagccacat atcggctatt accagttccg ctaaaatata aaatcataat aatatctccc
    29881 taactatttt ttatttatta tagtaattta ttgatatatt ctattcaaat gtatgtaaaa
    29941 tttagtagtt aagaaaatta taacacatta gtaattttta tacttattgg taaagaattt
    30001 gattaccatt aaatttagca acaagacatc aactggactt gtatatgaga agggataata
    30061 atttgcttat gaaagattat aaatataaag gttattttaa aggtgtagaa atatttgata
    30121 atgtaaatga ccttaactta aatactttaa caacagttag tagatttgtg cttgtttttt
    30181 tgtttacgat aacttaagga aatatgttac ctgaataata aatttatata ataaaagtat
    30241 ataaatatat atatgaatgt taagattagt tgatagaatt taaataataa atccaatatt
    30301 ataaacatag gaggtatgaa aaatatgatt aatgagaatt taaaaagtct aagaaaaatc
    30361 aaccagtata cacaagaaga attagctgaa aaacttaatg tttctagaca atcaatagca
    30421 aaatgggaaa gtggagaatc tattcctgat atatgtagtt gcattaaact tgcaaaattg
    30481 tacaatgtca aactagatga cttagttaat cattcagaag aaaaaacagg tattatagta
    30541 cctcctaaag gaaagttttt ctttggagca gttgtagttg gtgaacgtgg tcagattgtc
    30601 attccaaaag aagcaagaga agtatttaat ataaatgcag gagataaatt attggtactt
    30661 ggagatgagg aaagaggatt aggaattgtt catcaacgtg atttgataaa ttttataggt
    30721 catgtgggag tagtacgtaa agaagaatga tttaaaaaaa ttataaaagg aggtatgaaa
    30781 tatgagtaaa atagtatttt ttagcattcc tgcatatggt catactaatc ctactattga
    30841 agtagttcgt gaattagtgg atagaggaaa tgaggtattg tattattctt ttaatgaatt
    30901 taaagataaa attgaaggtg ctggagcaaa atttatttgt tgtgataaat accttccaga
    30961 attactccct ggagatgaga aaaaaattgg taaggatttt cctggattaa ttgagatgat
    31021 agtagatact actatttctt tagatgaaaa agtttgtaga gaacttaaag attttaatcc
    31081 agattgcatt gtatcagatt cattaagttt ttggggaaag ttatttgcac aaaaacttaa
    31141 tattccatat gtatgctcta caacaacttt tgcatttaat aaacatacag caaagttgat
    31201 gaaacaaaac tttatagaaa ttatacgtat gatttttggt gtaagacgca ttaataaaaa
    31261 aataaagctt ttacaaagaa aagggtatga agtaaaaaat tttatttcta tagtttctaa
    31321 tgataatgat acggatacca ttgtatatac atcaaaagaa tttcaaccaa tggtagaaac
    31381 cttctctagt aagtattcat ttgtaggtcc atcagtatca aagttgatta ttgaacctaa
    31441 agagagaaaa agaaagctaa tatatatttc gcttggtaca attaataata aaaatgttag
    31501 cttttataaa aactgtataa gtgcatttaa agattctgat gttgatgtga ttatgtctgt
    31561 tggtagaagt actgatatta aaagtttagg aaatatacca aataattttg aggtcaaaaa
    31621 tagtgttgaa cagattgcta tacttcaaaa aactgatgtg tttgttactc attctgggat
    31681 gaatagtgtt aatgaaagtt tatattatgg agtacctatg gtattatttc ctcaacattc
    31741 ggagcagaga atggtagcta agagagttgt tcatttggga gcaggaatta tgttaaaaga
    31801 ggataagtca gaaagtatta aaaaagcagt atttcaagtt ataaatgaca atgaatataa
    31861 agaaaatgca actaaactat caaaaagctt ttataatgca ggaggctcaa aaaaagcagc
    31921 agatgtaata ttacagatta tatgtaatag tagacaagtt gacatataat agatttgtta
    31981 aaaatatata tacatacatg taatttaatc aaagtactga cagtggggtc ttttaaccta
    32041 ccagttgggg catatatatt gatgctaaat atgagggatg ttatatttta ataaaaaaga
    32101 taacatcctt tttttagtgc attatatcga gttttaaata ataaatgaag gaggattaat
    32161 atgaaaaaat ataaagttat ttttatagta ttaattatag gtattataag tgtaacaggg
    32221 tttgttgtat ataaaaactg cattaaacca tggaaagatg ctgaaacata tattgataag
    32281 tatatggtaa aacaagatat ttcaaaagat aatataaaaa gtattacaaa agaaaaagcc
    32341 aagaaggcaa gttacgaagg aatactatac aaagtgtatt acaaagatga ccctcgatat
    32401 gagtatcaat atttttatag tgataattat tatgctctat atgtaaataa agtaatgtta
    32461 caaatttatg atatggaaaa taataataaa cttttaagaa gtaatgagga attaaaaagt
    32521 gtaaagtatc cactaatata tcttaaatat aagccagata aatgatttat gacaaaatag
    32581 aatgattttt attaaaattt atttaaaaat atcactagag cgtttaaata aaactaaatt
    32641 taagtattta tatattagta tatcttacta gtgtttaaac aaaattaaat ttaagtattt
    32701 atatattagt atatcttact agtgtttaaa taaaactaaa tttaagtatt tatatattag
    32761 tatatcttac tagcatttaa atgaaactaa atttgaagct ttacctatta atatatctta
    32821 atagtgatta aataaaacta aacttaagac tttatatgtt aatatatctt aagaatttat
    32881 ttgttaacat acagttcatt atatttaaaa gcattactat gataattcat aatagtaaaa
    32941 tagataatgg ttcagacaat attataaata ttagtgacgg taaaatagta tcaatttagt
    33001 atataaacct tgtcagataa tcgcataaca gagcttgtag ttatagaata aaaaaatatc
    33061 taggaaaccg ttgacagatt tatatggata gtataacata tagaatatga aattaaacaa
    33121 aaaagaattt taattatggc gacgtagtca taattttgag tatgttactg attagcaggc
    33181 agaactcaag tatatatgtg aaaaatagag ggatactcta tacatatatg cttgagttct
    33241 ttttattgaa aggaggaata tatgaaaata attcagattt taaataataa tgtagcctta
    33301 gtaaaaagag gtaaaaatga aattattgta tttgttaaag gaattggctt taaaaaaaga
    33361 gtgggacaag taataaatga aaatgaaata gagagaacat atatactaga ttcatacgat
    33421 atgctagaac actttagtta tctactacaa aactcagact ccaataatat agttcttatt
    33481 aatgaaataa ttgaatatgg tgagaaagct ttaaatataa aggcaagtga ttatctaagc
    33541 ttaaccttac tagaccattt agagttttta cttaaaagat gtgaaaagaa tcaatttata
    33601 aaaagtcctt tggtatggaa tgttaaaaga ttttatccaa agcattttga aattggaatg
    33661 catgcactaa aattaattgg tgatagtaag ggaattaatc ttcctgatga tgaggcagta
    33721 tcaatagcac ttcattttgt aaatatggag gtaaataaag agtctcatga tagtacaatt
    33781 gtagagttga gaactttagc agatatagtt tctataataa aatatcattt taatgtagag
    33841 ctagatgaaa cttcaacaaa ttatatgaga tttactactc atctacagta ctttatccaa
    33901 agaattatgt cggatgaagc ttcaatagat gaagataatt ctatagattt atataaacaa
    33961 gtaagtcaac tttatccaaa gtcatttgga agtgttcaaa agataagaaa atatataaaa
    34021 tcacaattta atgtcgatat atctataagt gaagaaactt atttaatgtt gcatataaat
    34081 agggtgactc aaagactgga ggcaaaatca gatgaaatat aaagatttaa atgaaaatat
    34141 aataaaactt atagggggaa aagaaaatat aaagtcggta gcgcattgtg ttactcgttt
    34201 aagatttact ttaaatgata ggagtatcgc tcaaacagaa gaaattaaaa aattaaaagg
    34261 agtaattgat gtagtttcta atgatgttgc ttatcaaatt ataataggta ctcatgttgt
    34321 agatgtatat aacgagttta tgtctatgat aggtttaagt tcaagtgaat caagtataaa
    34381 aagtgaaaga aaaaagggaa ttagaggtat caaaggtata tttaattcca tctttgtggt
    34441 agtctcagag actatgactt cagtaattga agtactactt gcagctggta tacttgcaag
    34501 tatattagca cttttaaata tgtctggcat acttcagagt gacagtccta catatcaaat
    34561 attagataca ttaagaagtg ctacatttca cttcttacca gtattaattg caatagcatc
    34621 agcaaaaaga cttaatgtaa atccctatat agcagtagtg ttagcagtta cgttattatc
    34681 ttcatcaata gatggtgtag aaggattaag tttatttggt ataaatttac aggctacaac
    34741 ttatgcaaac agttttattc caatactttt aggagtatgg gcaatgggaa ttataattga
    34801 aggtttgaaa aaaatattac ctaaagcatt acactatttt ttagttccag tgttatcttt
    34861 aataataact ctatcagtta cattaactgt atttggacct attggtatgt ggattggtga
    34921 tggattaaac tatgcatgta tgtttttaat tgatacattg ggtaattgga gtgttgttgc
    34981 actgtatgca gctttacaac cactcttaat agtttttggt gcagcaaact ttactatgcc
    35041 tatatcttta aattctgtga caacattggg gtatgaccca atttttatgg gcgctgcaac
    35101 aatatctgat attgcagttt gtggtgcaat gtttggatat ttcttaaaaa ctaggaataa
    35161 agaacaaaaa caactatttg gtacagtaag ttttagtgca cttatgggag ttacagaacc
    35221 ttcagtgttt ggtgtgttta tgaaatatag aagaccattt gtagcagtaa tgataggtgg
    35281 aggtataggt ggtttaatag caggtttagc tcaagttaaa acatatggaa tggtttgggg
    35341 attagctgca ttaccaacgt atcttgttgg aggagaggta tctaacttta catttatgat
    35401 aataagtgta gtagtatcat ttgtagcagc aacagcagct tcctatttac taggaatacc
    35461 ttcagatgaa aaagaagaag ttaagggtga taatgaaaaa gaattagaac taataatcaa
    35521 cgagaacaat tcaagaacta ttagtattgg aaatgttgca aaaggtcagg taattgcact
    35581 tggggaagtt aatgacaaag cattttcctc tggagcatta ggtaaagggg ttggagttat
    35641 acctagtgaa tcaaaatcaa ctgttatatc tcctgtagat ggaactataa ctatagtatt
    35701 tccaactaaa catgcatatg gtatagtgac tgatgaagga ttagaaatat taattcatat
    35761 tgggatagat actgtaaact tagatggaaa attctttgaa agtcttgtaa cacaaaatca
    35821 aagggttaaa aaaggagatg ctttagcagt atttgaatct gataaaatta tagaggaagg
    35881 ctttgattct actataataa ctgtagttac aaatactagc gattatttag atgtaatctc
    35941 atcaaatgat gaatcagaag aattattaac tgtaatagta taaattttag gagggaaaat
    36001 tcatgaatat taaagataca tttttctggg gaggaagtat tgcagcccat caatgtgaag
    36061 gttcatggga tagtgataac aaaggacctg caattatgga ctttgtaact aagggctctt
    36121 atgaaacgcc aagagtaatt actgataaaa tagaagaaaa attagattat ccatctcata
    36181 atggaataga tttttataat agatataaag aagatatagc tttatttaaa gaaatgggat
    36241 ttagtgcatt aagaatatct atagattggt caagaatttt tccaaatgga gatgatgaaa
    36301 atcctaatga attagggatt aaatactatg aaggtctgat agatacatta atagaaaata
    36361 atatagaacc tattgtaaca ctatatcatt ttgaattacc tatgaattta gtacataaat
    36421 atggttcatg gaataacaga aaacttatag atttgtattt aaagtacagt gagactgtaa
    36481 taagaagatt tgatgataag gtaaagtact gggttacatt taatgagatg aaccatattg
    36541 accctcaaac agagcactcg gatattttta cttatatgat agcaggtatt aagtatagtg
    36601 aaatagaaaa taaggttcaa acattggcaa atgtagggta taatatgact ttggcgggtg
    36661 taaaggttgc aaagttggct agagaaatca atcccaacaa taaaattgga tgtgtatttg
    36721 gactaaatcc aatttatcca tacaattgta atcctagtaa tgtgttaaat gcatttttag
    36781 ataatgaccg tgattattat caaattgatg caatgtgcaa tgggaagttt ccacaatata
    36841 aattaaaaga atatgaaaaa aataatataa ctattgaagt gactgatgaa gacaaaaaag
    36901 catttaaaga aggtaagtta gattttatag gacttaatta ttatttttca agtgtatcta
    36961 ctccaaaaga aaatttatca ggtgataaat ctttatttgg tgggattcaa aatccatatt
    37021 tggaacaaag taagtggggg tgggcaatag accctatagg tttaagattt accttgaact
    37081 atatttacag aaagtatcaa ttacctatct taattacaga aaatggttta ggggcatatg
    37141 ataaaatcga agaagatgga actataaatg atgattatag aattgaatat ttaagtaagc
    37201 atctatctca aatgaagtct gcaattgaag aagataatgt tgaatgtttt ggatatttga
    37261 tgtggggacc aattgattta gtcagtgcaa ctacaggtca gatgagtaag agatatggat
    37321 ttatctatgt tgacttagat gatgaaggca aaggaagttt taagagatat aagaaaaaat
    37381 catttgattg gtacaaagaa gtaataaaat ctaatggaga agttttagat ttttctacgg
    37441 atatagtagt agggtaggaa taagataaat agaagttagg aactaatgtt aattagtgtc
    37501 tagtaaagca tttaaaatag agtatagggt ctttaaagta aagtgtgtat tcttcttaaa
    37561 aataatggca attaaaatta ctataaaaag gagaatacac attttttatt tttatactta
    37621 agtaaatatt tagacatatt catagtataa tttactcatt atataaaaat agtatgattt
    37681 actcactata taactttata aattttctgg aaaaatgtca gaatatattg acaaagaaaa
    37741 gatttaaaat tataatctat attaagaata gtttgacaat ataaaaacaa gtatttttat
    37801 aaatagtgaa attattttta ttgtcaattt aagaataatt taggactact ataaagataa
    37861 aggtatagat gtcattatat gataaattat acttgtatag aaagaatttt aaagtatggc
    37921 ttatcattga tttaatataa taaataattt ttgtttttag taatagtctc taggaataag
    37981 atatctaaat tcgcttagaa atagtctaat ttacaaggga ggggatgcac taatagataa
    38041 aatttctata taaagaagac ttaaagtgaa gtatattctg ataatattgg t
//
LOCUS       DAEPIN010000035        38023 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.035, whole genome shotgun sequence.
ACCESSION   DAEPIN010000035 DAEPIN010000000
VERSION     DAEPIN010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 38023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38023
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.035"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            <1..606
                     /locus_tag="KR250_001967"
     CDS             <1..606
                     /locus_tag="KR250_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBH0067903.1"
                     /translation="ISGYLTKKLQDLNVDTIRTDILTSPTVTDVIVWNIEQSGTDTFS
                     ATYEVDQQIKEGEQTTTVKATYTVKVHVDADRDMVIIQNPTLAPAIEKSDYEPKTPEA
                     DASVDADTVNDATAFLETFFKLYPTATAKELAYYVFGNVLEPIGRDYLYSELVNPVLT
                     KDGDNMKVKVAVKFLDNQTKATQVLQYELILHKDSNWKIIM"
     gene            819..1676
                     /locus_tag="KR250_001968"
     CDS             819..1676
                     /locus_tag="KR250_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBH0067904.1"
                     /translation="MYKEVGISSNNVPITLSIWYTERSSPTIVFLPGTMSHPLMYENF
                     LCGLSERGFNIIGIHYLSHGKSPKIKKNYTMEDLLHNVYDATTYGIENFGENIAVMGS
                     SQGGILAAMAAGRDTRLKAVFPHNIMLTTLKETMSLTKYPTWSHRFMGLIQWGFRVGG
                     KLLPNYKVPGDAYLDYDRVFYSEQAKQDCLNDPMLLPYYPLRFVTSLFNADLSCLENG
                     AIQCPVILITAKGDPLFSFDYTNKVFDLIHVPQKELLALELNRHMIFNEDTDTTINAL
                     EATLHKYLG"
     gene            1754..2335
                     /locus_tag="KR250_001969"
     CDS             1754..2335
                     /locus_tag="KR250_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBH0067905.1"
                     /translation="MPRFTEQEKEIINSKLLIEGEKLFALHGLKKVTVDDLVAAVNIS
                     KGSFYAFYPSKEHLYVEINFRLQKELFTSIETTIKKKKYKNHRDLAKDVIMLSLTGVI
                     TSPILSQIDLSLMDYLQRKISSDIFENHMYSDIRILEILEDLGVVFLVPHTVIIKSLY
                     SVLSCLEQFKEDEELNMIQNLLVNGIIQQVVAE"
     gene            2439..3284
                     /locus_tag="KR250_001970"
     CDS             2439..3284
                     /locus_tag="KR250_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0067906.1"
                     /translation="MIDVKKLYFSYTDKPFVENVSFHVGRGEIFGFLGPSGAGKSTIQ
                     KVLTGLNTRYKGSVKVAGTEIREHTNQFYENIGVDFEFSTCYEKFTARQNLAYFASLY
                     EKQPRSIDELLHMVGLENDGDKKVADFSKGMRSRLNFIKALVHDPDILFLDEPTSGLD
                     PTNSRLMKDIILAEKKRGKTIIITTHNMFDATELCDQVAFIVAGKVSALDSPHNLIMS
                     RGAAKIQYTYYDKGEKTGECLLDRTTEDKLLKSLISENRLLSIHSSEPTLNDIFVDIT
                     GRTLQ"
     gene            3281..3964
                     /locus_tag="KR250_001971"
     CDS             3281..3964
                     /locus_tag="KR250_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016295773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0067907.1"
                     /translation="MRLGRLICGDIHFQWKYGFYFIYFILTVLYVCGIAALPEHWKTD
                     IASIMIYSDPAAMGLFFMGAIVLLEKSQKVLNAMVVSPVKISEYILSKTVALIAISTV
                     IALILGVVSGSNHLLGIAVGTALTSAIFTMLGIIAATKISNLNQFLIVIMPIEIICFV
                     PPIVGLFVKLPYLFRFFPFTACMNLITGKSVLLSFDMVLVIATLIILYIVARHTVEHM
                     WKSLGGVKL"
     gene            3961..4659
                     /locus_tag="KR250_001972"
     CDS             3961..4659
                     /locus_tag="KR250_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0067908.1"
                     /translation="MKKILSSTIQIFKQIKSDPMMFAACFSPFIMGTLIKFGIPFLER
                     ITKFSLQAYYPIFDLLLSIMAPVLLCFAFAMITLEEIDDKVSRYFSITPLGKSGYLFT
                     RLGVPSIISAVIAFIVLLLFSLEKLSIGMTICLALLGSVQAIIVSLMIITLSSNKLEG
                     MAVTKLAALTLLGIPAPFFIDSYYQFAVGFLPSFWVAKAMQNEAVLYFSIGLVVALVW
                     YYFLTKRLFRKLAG"
     gene            4671..5582
                     /locus_tag="KR250_001973"
     CDS             4671..5582
                     /locus_tag="KR250_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBH0067909.1"
                     /translation="MEKGRLLQNKNMNKAIQVSKITLIINLSLSLLKFAAGYIGKSSA
                     MLSDAVHSASDVVSTIVVMVGIKISEKQPDREHPYGHERMECVASIILSVALAITGAG
                     IGYSGIKKIFSGQYNTLSVPSGIALTAAVLSIVIKEWMYWFTRSAAKHTNSDALMADA
                     WHHRSDALSSVGSLIGILGARLGYAILDPIASVVICGCILKAALNIFKESINKMVDHS
                     CDNATETRIREVVLQQQGVDGIDELKTRMFGAKMYVDIEILADGNLALYDAHRIAEGV
                     HQTIENNFPQCKHCMVHVNTNELLYSA"
     gene            5634..5774
                     /locus_tag="KR250_001974"
     CDS             5634..5774
                     /locus_tag="KR250_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0067910.1"
                     /translation="MEQYWWIIIVVVLIVVGLLKIVIMKKYNQRRNSNNRSKDSEDDK
                     MY"
     gene            6065..6691
                     /locus_tag="KR250_001975"
     CDS             6065..6691
                     /locus_tag="KR250_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBH0067911.1"
                     /translation="MSDTKENILKIALHLFAVNGYEAVSVSMIAEALGMTKGALYKHY
                     KNKQDIFDCIVERMYQIDNKRAKDYQVPESTIEVTPNAYRNTTIGSLITFTLAQYDFW
                     VTDEFASNFRKMLILEQYRNLKMNMLYQKCLVNGPISYIEDIFNEMIEKGVLQECDTK
                     QLAIEYYAPLFLLISVADAVENCWDLKTQLTTHVKQFVKKYIINEKVK"
     gene            6813..7529
                     /locus_tag="KR250_001976"
     CDS             6813..7529
                     /locus_tag="KR250_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBH0067912.1"
                     /translation="MEYIRSKKYFTPDLQTKIMGPNPIKLQEELLQNHKIPSGSYVCD
                     LGSGQGLTSVFLAKEYGFVVFAVDLWSEPKENEEFFKKMGLSSAQIKSVKADANELKF
                     EKEFFDAVVSTDSYNYFGRDPIYLDEKLLPFVKKGGFVYITVPGMKKDCHEKLPEELL
                     LSWTPEELDYIHDITYWSNIISHCKKADLISIYEMESNSEVWSDWIKQNNEYAINDRK
                     AIEAGGGDYLNFIAIILQKK"
     gene            7679..8275
                     /locus_tag="KR250_001977"
     CDS             7679..8275
                     /locus_tag="KR250_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015541496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HBH0067913.1"
                     /translation="MKILWIIIIMADNLLPLLLARFYPNYKHKEMALSVLGSRQSPVK
                     WIYNVWCIISGVVFCIAPYALYQENDSGWAIAIWMLLAIYGIGCEIISGVCPLNENRQ
                     DEDIITKIHGGTSAIGFMALLVVPLLFAILQFQTTKVVLGAISLVCFTIAFIFFCFFI
                     MGEKEKFANTVLRYGGLWQRLVLISCYIPLFVWSVSNQ"
     gene            8362..8553
                     /locus_tag="KR250_001978"
     CDS             8362..8553
                     /locus_tag="KR250_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003064779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HBH0067914.1"
                     /translation="MKKYEYVEINYNAKGVVFLCTDKHKEIIDSYAKTGYHYAGFVPT
                     EIDAKGCMRKIDLIFEKED"
     gene            8577..8807
                     /locus_tag="KR250_001979"
     CDS             8577..8807
                     /locus_tag="KR250_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0067915.1"
                     /translation="MIGLKRRDMNYIMKVGAKAQGVSVSEFRAMLQDTIDKTMESTDP
                     DVQANFKRYFGNKRPTPEEYIYTITKKTKVKV"
     gene            9074..9493
                     /locus_tag="KR250_001980"
     CDS             9074..9493
                     /locus_tag="KR250_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009261829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBH0067916.1"
                     /translation="MKPSDFQKTIQCQFECKLKKVVKGIIWNYRKELKRRKNKEISFS
                     ELPDITVESLATWDDYETNYTIFNVCGIDIKVLDDELAEALKQLPEKKRNNVLMYYFL
                     EMSDTEIADIMNYTRGAVFKHRHRALETMKEILKEKK"
     gene            9496..9750
                     /locus_tag="KR250_001981"
     CDS             9496..9750
                     /locus_tag="KR250_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH0067917.1"
                     /translation="MKKKYKHPSYYLISSAMDGDETAIEKLLAFYDPYISKCCLRPLY
                     DEYGNVYIVVDMELKGRIREALIKMILDFEIDIDIETEEE"
     gene            10125..10328
                     /locus_tag="KR250_001982"
     CDS             10125..10328
                     /locus_tag="KR250_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HBH0067918.1"
                     /translation="MNNTDVPIWEKYTLSIEEASRYFRIGEKKLRKLAEENLDSGWVI
                     VNGNRIQIKRKQFEKIIDTLDEI"
     gene            10406..11596
                     /locus_tag="KR250_001983"
     CDS             10406..11596
                     /locus_tag="KR250_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006354938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBH0067919.1"
                     /translation="MSKMKRQDSKGRNLRLGESQRKDGRYVYKYTDIFGKPQFVYAWK
                     LVPTDKTPTGKRDDKSLREKEKQIKKDLDDGIDTIGGKMTVCQLYAKKNNQKKNIKRN
                     TEIGRQYLMNALENDPLGMRAIDTVKQSDAKEWAIRMNDKGYSYKTIDNYKRSLKASF
                     YMAIQDDCIRKNPFHFKLSDVLEDDTEPKVILTPEQEEKLLSFMETDNVYSKYRDEVI
                     LLLETGLRISELCGLTTHIDMLNRVINIDHQLLRDTEVGYYISTPKTKNGKRELPLTE
                     RAYQALERILKNRGKAQPLVVDGYSNFLFLNREGLPKVAGNYESMVRGLIKKYNKTHE
                     DKLPNITPHSFRHTYCTNMANKGMNPNTLQYIMGHANITMTLGYYAHGTFQSAKAELE
                     RLAS"
     gene            complement(11853..12653)
                     /locus_tag="KR250_001984"
     CDS             complement(11853..12653)
                     /locus_tag="KR250_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0067920.1"
                     /translation="MRLNKRSEGYINYVKGVKREKRNVLFYQLLILIGFIVIWELLAD
                     LNVINTFLFSKPSDIYNLFIQYAFSGQLFKHIGISVYETVLGLVIGTVLGILVAIALW
                     WSEKLSKILDPFLVVLNALPKTALAPIIIVWVGAGIEGIVVTAVTISVVVTILSAYNY
                     FMNIDEEKIKMLKSFGATKSQILFKLILPANTGNLINLTKINIGMAWVGVIVGEFLVS
                     RYGIGYLIVYGSQVFKLDLVMMGVFVLAICAWAMYAVVNIIEKIYNSR"
     gene            complement(12848..14446)
                     /locus_tag="KR250_001985"
     CDS             complement(12848..14446)
                     /locus_tag="KR250_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBH0067921.1"
                     /translation="MKAAIYSRKSRFTGKGDSIENQIQLCIDYAKSIGVKDFLIYEDE
                     GFSGSNIDRPQFKKMMQDAKDKKFSYLVCYRLDRISRNVSDFSTLIEKLNELSISFIS
                     IKEQFDTSTPMGRAMMFISSVFAQLERETIAERIKDNMYELARSGRWLGGNTPHGFNS
                     EKITFLDENLKERSMYKLQVNDEQMEIVKLIYNKYLELRSLSKLYKYVYNNGIRGPRG
                     GKFDPSSLSRILRNPAYVKANDEILDYLKNSGMDVVGTPDRKCGILTYAKNTSNSIAA
                     IAKHHGVIEPEIWLEVQNQLDKNRDKTPRISTGKTALLSGLIKCGKCGSNMRITYKGK
                     KTDKDLKYYYICGTKKTLGSDSCNCKNLNGPLVESLVIDKIKNCKEESIISAFNSNNI
                     KTDNVFNIFKSETNLLKNQILDKERLIKNLVIELAKNTGSTASDYIVSQIESLNNEIS
                     ILKDKINILESSNINISSDSINIDIIVSNLKRFNDEVDNASLDDKRLLLSTIINSIVW
                     DSSLGNLNIVYMGAEVDNSVSLSN"
     gene            14774..14950
                     /locus_tag="KR250_001986"
     CDS             14774..14950
                     /locus_tag="KR250_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0067922.1"
                     /translation="MEINVIEIFPKDKAKLNKIEMDKASWFVNIISKKYPKEALNEAF
                     STLEKELNISKANT"
     gene            15124..16254
                     /locus_tag="KR250_001987"
     CDS             15124..16254
                     /locus_tag="KR250_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HBH0067923.1"
                     /translation="MNNFEIKTRIKFGEGSLKALQELKNKNVLIITDPFMVKSKTIDK
                     ITSNLINSTYQVFEEIVPDPPIEVVVAGIEAFKSINPDVIIALGGGSAIDAAKSIMDF
                     SKKILKINEVEFIAIPTTSGTGSEVTSFAVITDSQKKCKYPLVSDDLLPDMAILDPEL
                     VKTVPNFITADTGMDVLTHAIEAYVSTNSNDFSDALAEKAIKLVFKYLIKAYKDGNDI
                     EAREKMHNASCLAGMAFNQASLGINHSIAHVLGGKFHIPHGRTNSILLPHVIEYNSSI
                     VGYTNTTYSATAKKYAQIAELVGLGSANIRISVRNLVNEIKKLQKEMNMPTKLTQCKL
                     SLEDITNEEREIAKLAIKDTCTLTNPKVPTEDDIINILKNIK"
     gene            16495..16845
                     /locus_tag="KR250_001988"
     CDS             16495..16845
                     /locus_tag="KR250_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003453843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HBH0067924.1"
                     /translation="MTEESKQRVIQEYVPGKQVTLAHIIANPNEDIYKKLGLVLDKKD
                     AIGILTITPSEASIIAADVATKASNVSLGFIDRFSGSVVISGDVSSVESALNDVLEVL
                     GNMLNFSSTKITRT"
     gene            16853..17302
                     /locus_tag="KR250_001989"
     CDS             16853..17302
                     /locus_tag="KR250_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HBH0067925.1"
                     /translation="MKNIIFMGKTGSGKTTLCQKLDQLELKYKKTQSVELYNNAIDTP
                     GEYMENRNLYNALITTSADAKIIAIVYDATQEENYIAPGFASMFCKDVIGIITKINKA
                     TENEVNIAYERLSLAGASQIFKIDTVDNIGLEKLFKYLEKYKNIDEV"
     gene            17404..17979
                     /locus_tag="KR250_001990"
     CDS             17404..17979
                     /locus_tag="KR250_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBH0067926.1"
                     /translation="MGKKILLVDDEPLLRMDTKDILESHGYEIIGEASDGFEAVEMCK
                     KHKPDLVIMDIEMPILDGIKAGKIICKENIAGGVLLLTSFEDKEYVEKAKSIGAYGYL
                     VKTASEKSLIPTIEMCLNKVNEFEKMKKDLEKVNSKLNERKLVERAKGILIREFKISE
                     DEAYTRIRKLSMDKRTTMMEIAKMIIIGYDD"
     gene            17972..19381
                     /locus_tag="KR250_001991"
     CDS             17972..19381
                     /locus_tag="KR250_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal domain-containing
                     protein"
                     /protein_id="HBH0067927.1"
                     /translation="MTNTIKKISTAQTYLNNNEIEKIIEVSKSLDLMASFYEADVFID
                     VLSKFDDEAIVVAHGRPKEKSMYCENVIGKKALIENEPGVIKCLKTGERVRDIKALTQ
                     EYKLVKQNIQPITLDDKVLAVLIVEKDISSEVKNEFNASEDEENKNLHQFIKLIGNND
                     FLVDNLNSAILIFDKNGKLKIKNRIATEIYKNLGFGYDIQDMHYNDLDIETNTFENIL
                     MDENYDETNEVSIGNMFFKIKTIYIKDDEFKVCKIVQDISDLKRKEEELVLKTVAIRE
                     AHHRVKNNLHTVISIIKKQSRLSQNDEVKQCLDNIINRVFAILSTHYLLSKEVDNNIS
                     IREGINLLVSNIQGGYLFSKDIDIRITGEDFKISGEKATAILLVINEILQNCYDHAFS
                     DREHGTIQILINKDNDYRNIAIIDDGIGFDSNNINKNSLGTYIIDSYITQMLKGNIER
                     KSSENGTEVLITIPTQINI"
     gene            19565..20998
                     /gene="eutA"
                     /locus_tag="KR250_001992"
     CDS             19565..20998
                     /gene="eutA"
                     /locus_tag="KR250_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004458698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HBH0067928.1"
                     /translation="MREELLSVGIDIGTSTTQLIFSKLIVENTESNFSVPRISIVDKE
                     IIYKSKVYFTPLISNNEINGQQIKDIVENEYKKANISKEDIHTGAVIITGETARKENA
                     NDVLHTLSGFAGDFVVATAGPDLESIISGKGAGAHIYSKEHSTTIVNIDIGGGTSNIA
                     SFKRGEVTDTGCLDIGGRLVKIDKISKKITYISPKIDKIIKDNNLNLQVGTMATVENT
                     KPIVDIMVDLLMESIGLKKQTPLFDFIITNKSISIDENIKNVSFSGGVADYIYYDGEI
                     KDYFEYGDLGILLGQAIKNCEYLKKVNVVKSMETIRATVVGAGSHTTEVSGSTITYTS
                     EKFPLKNLPVLKLSIDEETGTSKQISEAIKNKLNWFKLENDLQKVAIAMIGKKNPTFL
                     DIQEYAKGFVDGMQQIIENDLPFIVIVENDMAKVLGQAIYALLNYEKEIVCIDSVKVE
                     NGDYIDIGKPIVEGKVLPVVIKTLVFN"
     gene            21016..22380
                     /locus_tag="KR250_001993"
     CDS             21016..22380
                     /locus_tag="KR250_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutB"
                     /protein_id="HBH0067929.1"
                     /translation="MILNTKLFGRTYQFKGLYDVMAKANEEKSGDKLAGLAAESAEER
                     IAAKVVLSHIKLEDIRNNPAVAYEEDEVTRIIQDAVNEKVYEEIKNWTVSELREWLLD
                     SNTTSLDIRRISRGLTSEMIAAVAKLMSNMDLVYAAKKIKVTAHCNTTIGEQGTLSAR
                     LQPNHPTDDPDGILASLLEGLTFGIGDAVLGLNPVDDSVESVTKVLKRFEEVKQKYQI
                     PTQTCVLAHVTTQMEAIRNGAPADLIFQSIAGSEKGNEAFGFNGDTIEEARQLALKHG
                     TATGPNVMYFETGQGSELSSEAHHGIDQVTMEARCYGFAKKYEPFLVNTVVGFIGPEY
                     LYDAREVVRAGLEDHFMGKLHGLPMGVDVCYTNHMKADQNDMENLAILLTTAGCTYFM
                     GIPHGDDVMLNYQTTGYHETASLREMFGLTAIKEFDAWMEKMGFSKDGKLTELAGDAS
                     VLLG"
     gene            22393..23274
                     /gene="eutC"
                     /locus_tag="KR250_001994"
     CDS             22393..23274
                     /gene="eutC"
                     /locus_tag="KR250_001994"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014952556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutC"
                     /protein_id="HBH0067930.1"
                     /translation="MNEKDLKALVEQLVGQMLGELDTNVVSETVKKATEVVVDNNACI
                     DDITEVDIRKQLLVKNPKDAEAYLDMKAKTPARLGIGRAGTRYKTETVLRFRADHAAA
                     QDAVFSYVDEEFIKENNMFAVETLCKDKDEYLTRPDLGRKFSPETINNIKSKFGTNQK
                     VLILVGDGLSSAAIEANLKDCVPAIKQGLKMYGIDSSEILFVKHCRVGAMDHLGEELG
                     CEVICMLVGERPGLVTAESMSAYIAYKPYIGMAEAKRTVISNIHKGGTTAVEAGAHIA
                     ELIKTMLDKKASGIDLK"
     gene            23294..23947
                     /gene="eutL"
                     /locus_tag="KR250_001995"
     CDS             23294..23947
                     /gene="eutL"
                     /locus_tag="KR250_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003761789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HBH0067931.1"
                     /translation="MKNDVIRPNILGIKIISNISPEMAQKLELKSHHKSLGLITADCD
                     DVTYTALDEATKASEVDVVYAKSMYAGAANASTKLAGEVLGIIAGPSPAEVRSGLNAV
                     VDFLEYGATFISANDDDSIAYYAHCVSRTGTYLSEVAGIREGEALAYLVAPPLEAMYA
                     LDAAMKAADVKMCELFAPPTETNFGGALLTGSQSACKAACDAFAEAVKSVADNPTGF"
     gene            23958..24659
                     /locus_tag="KR250_001996"
     CDS             23958..24659
                     /locus_tag="KR250_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HBH0067932.1"
                     /translation="MDAIGLIEVIGYVAAIEASDACLKSANVNIVRVDKVGAGIVTLT
                     ICGDVGAVKAALEAGEIAASRVGTLRTSHIIPRIHNEVTNALFKTKEPKVCEVSEDKE
                     KNFEITLEIADNVSKNLSEKENVILDVNENSGDTENLYSDIDKTNKKIETVDDSLTEA
                     NEEKIENTNDKEVLEQVKIENSDFDRDDLSKNSVKKLKAMAKKLDSSITYKDLNVLKK
                     EELINLVKKLSRRDR"
     gene            24662..26131
                     /locus_tag="KR250_001997"
     CDS             24662..26131
                     /locus_tag="KR250_001997"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase (acetylating)"
                     /protein_id="HBH0067933.1"
                     /translation="MTLLDKDLVSIQEVRTLVRRSKVAQEKLSKMSQEQIDKIVKAVV
                     DAAFNNATKLAKMANEETGFGKWEDKVIKNTFASMGVYETLKNTKTIGIIGEDKEANT
                     IDIATPVGVVAGLIPSTNPTSTVIYKTIISLKAGNTIVFSPHPNAKNCILETVNIISE
                     AAIKAGAPEDSILCITNPSIVGTNELMKHKDTNLILATGGEAMVRAAYSSGTPAIGVG
                     PGNGPAFIDKSANVKLAIKRIIDSKTFDNGVICASEQSIVIEKSMEETVLKELKNQGA
                     YVLNEEESERLAKFILRANGTMNPQIVGKSVEVIANLADLTNVPKNTKVLVARENKVG
                     HNVAYSREKLTPILAFYVEENVEAIMNRCREILLNEGAGHTFSMHVNDEELVKRFALN
                     MPVSRILVNTPSALGGIGASTNLVPALTLGCGAIGGSSTSNNIGPLDLINVKRVAYGI
                     KEIEELGRDKSQVEKSMFDSMDKEELINLLVQKLVKELA"
     gene            26227..26514
                     /locus_tag="KR250_001998"
     CDS             26227..26514
                     /locus_tag="KR250_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HBH0067934.1"
                     /translation="MASANALGMIETKGLVGAIEAADAMVKAANVQLVGKEQVGGGLV
                     TVMVRGDVGAVKAATDAGATAAERVGELISVHVIPRPHFEVDAILPKVSAE"
     gene            26641..27402
                     /locus_tag="KR250_001999"
     CDS             26641..27402
                     /locus_tag="KR250_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin adenosyltransferase"
                     /protein_id="HBH0067935.1"
                     /translation="MGVLTENEVKRKIGKNKNNITEFYIEKGQIITPSAKSYLAERSI
                     ELKYVDKLSDVNNEPKEVVKEVVKEKKYKYTTVFGAKLDEKPEHMTQLNGNLLVFKDH
                     KRIILRGKIDSLESEILKVQILCQKLDMPKLVKELQEVLDFVRNIMRCEVLDEKLGDF
                     KLLGLTPQELREQSHYPKKYFGIGHEFPSYEMGEVVVAINSIRSNTREVELAAYEAFK
                     GEYGNVHREDLIKALNRLSSVFWIMIYKVRTNKYK"
     gene            27415..28044
                     /locus_tag="KR250_002000"
     CDS             27415..28044
                     /locus_tag="KR250_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HBH0067936.1"
                     /translation="MENLLNEIVEEVITRVKKEAFIEVEASGRHVHLSREDVDKLFGE
                     GYTLTKLKDLSQPGQYACKERVTITGPKGSIKNVVVLGPCRNETQVEISLTDGSTLGL
                     KAPIKQSGDLEGTLSIKISTQYGEVELDKGLMVAKRHIHMTPKDAEKFNVCDKEIVEA
                     KVMGQRPLTFDDVVIRVSDSFKTYMHIDYDEANACGYSKGTVAKIIKKA"
     gene            28086..28811
                     /locus_tag="KR250_002001"
     CDS             28086..28811
                     /locus_tag="KR250_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02536 family ethanolamine utilization
                     protein"
                     /protein_id="HBH0067937.1"
                     /translation="MNYDNLVDLITEEIYKKINSNEIKISNKPKAVIVFEQDNNKFNL
                     LKDGFEIVEFDKSIRECEIVIVSRLCMRGLSNIALGNSTSDEERFILKMIMNGKKVYV
                     LDEGIEYKKYKDTAPKALYKKFMSFEDEICKFGVEIIKDLNVITKNKLNIKKELESTR
                     GSKKEDTKVLDKDILNLTSVESVKIDNEFSLDLRNKKLISEADLRKPTINGVKNILVN
                     KKSIITPLAVDFMRIHHLKLKKL"
     gene            28824..29096
                     /locus_tag="KR250_002002"
     CDS             28824..29096
                     /locus_tag="KR250_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HBH0067938.1"
                     /translation="MDIGKVVGNVWATRKDEKLCGQKLLVVKILKTKDTYKEGFMVAA
                     DNAGAGNGDLVLISKGSSARESIEKSHVPVDATIVGVVDSFEVFDE"
     gene            29089..29640
                     /locus_tag="KR250_002003"
     CDS             29089..29640
                     /locus_tag="KR250_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HBH0067939.1"
                     /translation="MNKSIGAIEFKSIAKGIEVSNEMIKKSSVDVLYLKSICPGKFLI
                     IVGGETSYINECVDYGIKLGEGYIVDNFVINAISQEILDGFKNKYQKLDSIVSIGVVE
                     SSKICAGIKMLDKTLKSGDLVLVKLQLSFAIGGKLVYIVAGDLSSLEYALKESENVVR
                     EKEVIYKTVIPSVDSQIIKSLIK"
     gene            29652..30743
                     /gene="eutH"
                     /locus_tag="KR250_002004"
     CDS             29652..30743
                     /gene="eutH"
                     /locus_tag="KR250_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HBH0067940.1"
                     /translation="MSINEIIIYVMVVFMVLGAIDKCIGNKFGLGEQFEEGIMAMGSL
                     AVAMVGVICLAPVLADVLRPIVVPVFNILGADPAMFAGSLLANDMGGAPLALELAKDP
                     NAGLFGGLIVGAMMGPTIVFIIPVALGIIEKEDQKFLATGILAGIITIPIGAFVGGLV
                     AGFEVMMVLRNLVPIIIFAVVIALGLLKFENAMIKGFTYFGKGVVIVITLGLAAAIVE
                     ALTGIVVIPGMKPISEGIEIVGDIAIVLAGAFPLVFVITKVFNKPLMALGKVLGMNDV
                     SAAGLVASLANCIPMFGMMKDMDNRGKIINVAFSVSAAFVFGDHLGFTAGFNSNMITP
                     MIVAKLVGGITAVLLAMVIANKTLKKENV"
     gene            30745..31218
                     /locus_tag="KR250_002005"
     CDS             30745..31218
                     /locus_tag="KR250_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF861 domain-containing protein"
                     /protein_id="HBH0067941.1"
                     /translation="MDISNIDKNLIETLVRQIIEEKISGTKDTVDFVRNKDISGITSI
                     KLPTVKVSESDRLDTGNPSDVVYTKDLFTLEESPRLGCGMMEMKETTFDWTLNYDEID
                     YVIDGTLDIIIDGRKVSASSGELIFIPKGSKIQFSVPDYARFIYVTYPADWASQN"
     gene            31731..32252
                     /locus_tag="KR250_002006"
     CDS             31731..32252
                     /locus_tag="KR250_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBH0067942.1"
                     /translation="MEEIVKKEQASMNDIFLYLKAKSNSLYKFVILYNEFINKPKDYG
                     TGELINMVEVHMLTAIEENPGISPTQLAKIWNRTKGAISQTISKLEKKGYITKEKKEG
                     NAKTILLYPTTSGVELSKAHKLYDTIDVTRTFEYLIKECTLEEIDSFYKVIDLFINLL
                     EESPQENNDSEKV"
     gene            32631..33794
                     /locus_tag="KR250_002007"
     CDS             32631..33794
                     /locus_tag="KR250_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBH0067943.1"
                     /translation="MNLKQLSEKYEEYVIQMRREFHKYPELSFKEIRTTQRIREELQK
                     IGVPFVSIEPNIVIATIQGKNEGKTIAIRGDIDALPMQEDSDVEYKSEYDGIMHSCGH
                     DAHGAMLLGLAHMLNDIRKDLKGRVLLVFQAAEEVGGGHKEIIQYFKENGGPDRLIAT
                     HVWSDIEAGKISVEPGPRMAGGSGWRIDITGRGGPDQSIDPIKPACDIVLKVSSIPVN
                     HISVLEQCVVHACAIHGGTTIGNVIPNNAEVIGGYRYFTEESGKKVFDIIKQMSNGVG
                     QVYGVDVKVTHTGGIGPVINDEEAVKMAKEIVSNIDGLELDSYEPICASDCYGEILKE
                     YSGFYCYLGVGNKEKGIIYAQHHPKYNIDEDILKLGCEFMAKYAVEFLNKNNN"
     gene            33828..35366
                     /locus_tag="KR250_002008"
     CDS             33828..35366
                     /locus_tag="KR250_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HBH0067944.1"
                     /translation="MQGALKENKKKSGGGFLGWIEKVGNKMPHPLALFTYITLIVVAL
                     SAIASFLGFSATSPATGKLIKVMNLISAKGLVLFMQEFVKNFQNFPVLGVVLVMAVAT
                     GVCEKTGFFSTAIKMSLSKVKGNAVVFIIAFIGVFANQAGDAAFVLVPTLAGAIFYGL
                     NRNPLAGIFCGFASVGGGFATAVIPGGWDVTLTPITVSAAQTIQPAFDMTLLASYYAL
                     FVSAFIVATVSTIVTVKFIEPKLGKYTGKPEGIDDNQGFEVTKEQAKAAKLAGFTVLA
                     YVALLIALCIPANSFLRSPEGSLIFGAPLMSCLQFFIVILFFLPGIVYGMRVGKIKTV
                     KDLNDILCQSMAAMAPFIVLAIVIGQFLTLFSVSNLAQVLAIKGGALLNSLPLPPQAI
                     ILLFLVLVAFINIFVISGSTKWMIFGPVFVPMLMQLNIHPAFTQFVYRLGDSVTNHLS
                     PLNAFFIILLATVQKYDKNAGMGTIFSAMIPYTIGYFVVLAVLVVVWMTIGIPVGIGG
                     QVWL"
     gene            35398..36600
                     /locus_tag="KR250_002009"
     CDS             35398..36600
                     /locus_tag="KR250_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBH0067945.1"
                     /translation="METTEIRYNFDEVIDRSGTNCLKYDLKSQFMPESPEDALPLWVA
                     DMDFACAPEIINAMHERIDRKIFGYSSQQTDDYYEAVCGWFKRRFDWKIEKENIFFSP
                     GVVPALGFFTQILTQKGDGIIIEKPVYYPFEDKITNNERKVVNSPLKFEDGHYSMDYD
                     GFEELAKDENNKVFILCSPHNPVGRVWKKEELRKIVDICKKYDLWIISDEIHCDIIRK
                     GEKHIPLEVACPDYKDRIITCTAPSKSFNLAGMQLSNIVINNKELQEKWMLETDSKLA
                     IFLPNPFAIVATKVAYNDCEDWMNQVNEYIDGNIKYVEDFVNEYMPKVKIIKSQGTYL
                     VWLDFRGYGLDAKQLEYVMLNKAKVLLDEGYIFGEEGAGFERINVASPRTIIEECMNR
                     IKCAFEGL"
     gene            complement(36767..37366)
                     /locus_tag="KR250_002010"
     CDS             complement(36767..37366)
                     /locus_tag="KR250_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBH0067946.1"
                     /translation="MNDNIVEVKNINMNFYTKEGELEVLKDVNFNLKEGEILTLLGPS
                     GSGKSTILNILTNLLKPTSGDIKITGNIGYMFQKDNLLEWRNIMDNITIGLEIQGKKD
                     KKSLDRVESLLKTYGLWDFRSMYPKELSGGMRQRVALIRTLSVNPDILLLDEPFSALD
                     YQTRLLVCDDVYSIIKNENKSTILVTHDIGEARCIKVQL"
     gene            37536..>38023
                     /locus_tag="KR250_002011"
     CDS             37536..>38023
                     /locus_tag="KR250_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0067947.1"
                     /translation="MSNIITNLIESIFTNPITILFLLAITVYSVFKIIENNRVYSFVS
                     SKLDEINRDYKTSEFYKKVRDDYYTYSKENPYADVNITSFIEEVVSDLKHNNLPLLEK
                     IRSIKNSSSISILLGVLGTFVGLSTMLLCVDTKDIINSLPSTISSMQTAFTTSIFGVV
                     FSL"
BASE COUNT        14353 a         5043 c         6964 g        11663 t
ORIGIN      
        1 atcagcggtt atctaacaaa gaaattgcag gacttgaatg ttgatactat tagaaccgat
       61 atactgacta gccccacagt tacagatgta attgtgtgga atatcgaaca gtcgggaacg
      121 gacacttttt ctgctaccta cgaagtagat cagcagataa aagagggaga acagacaaca
      181 acagtcaagg caacctatac ggtaaaagtc catgtagacg ctgacaggga tatggtaatt
      241 attcagaacc ctacccttgc accagcaatc gaaaaatcgg actatgagcc aaagacacca
      301 gaagcagacg caagtgtaga cgctgatacc gtgaatgacg ctaccgcatt tctggaaaca
      361 ttctttaagc tgtacccgac agccacagca aaagagcttg cctattatgt atttggaaat
      421 gtgcttgaac ctatcggcag ggactacctt tattcagaat tggtaaaccc agtacttaca
      481 aaggacgggg ataatatgaa agtcaaggta gctgtaaaat tccttgataa tcagacaaag
      541 gcgacgcagg tattgcagta tgagcttatt ctacataagg atagtaactg gaaaattatt
      601 atgtaagaca ttttgttggc aggatctatt ttcggatata gtctgccttt tctgtagcaa
      661 atgtactgca ctgacaagct atttcattgt attttaagca attaaacctt tcttgacaat
      721 tttatcttaa atcatatatt tatggatatt gactttctgc ttgcaataag ctataatatg
      781 actataaaat caaatcagtc atataggagg tatgtggtat gtataaagaa gttggtattt
      841 cttcaaataa tgttccgata acactttcta tctggtatac agaaagaagt agtcctacca
      901 ttgttttttt gccgggtact atgtcacacc ctctcatgta tgagaatttt ctatgtggtt
      961 tgagtgaaag aggatttaat ataataggca ttcactatct ttcacacgga aaaagcccta
     1021 aaatcaaaaa aaattataca atggaagatt tgctgcataa tgtatatgac gcaacaactt
     1081 acggaataga aaatttcggt gaaaacatag ctgtcatggg ttccagtcaa ggcgggattt
     1141 tagctgcaat ggcggcaggt agggacacaa ggttaaaagc cgtttttcca cataatatca
     1201 tgctgaccac attaaaagaa actatgagct taacaaaata tcccacatgg tcgcaccgtt
     1261 ttatggggct gatacagtgg ggctttcgtg taggtggaaa attgctgcct aactacaagg
     1321 ttccaggaga cgcttattta gactatgaca gagtatttta tagtgaacag gcaaaacaag
     1381 attgcttaaa tgaccctatg cttttaccct attatccttt aagatttgtc accagcttat
     1441 ttaatgctga cttatcgtgc ctggagaatg gggctataca atgtcctgtt attttaatta
     1501 ctgccaaagg cgacccgctt tttagctttg attataccaa caaagtattt gatttaattc
     1561 atgtaccgca aaaggaatta cttgctttag agcttaatcg tcacatgatt tttaatgagg
     1621 acacggacac tacaataaac gcattggaag ctaccttgca taaatatctt ggatagcata
     1681 ttgattatat aatcagtaaa tgttatactt ttcatgacga tataaatatt ttagtcataa
     1741 ggaggttatg agtttgccac gatttacgga acaagaaaaa gaaatcataa acagtaaact
     1801 tttgatagag ggagaaaagc tgtttgcatt acacggctta aaaaaagtaa cagttgatga
     1861 tttagtagct gctgtaaaca tatcaaaagg ttctttctat gctttttatc ccagtaaaga
     1921 acatctatat gttgaaatca attttcgctt acaaaaagag ttatttacaa gtatagaaac
     1981 aaccattaaa aagaaaaagt ataagaacca tagagattta gcaaaggacg ttatcatgct
     2041 aagtttaacg ggggtgatta cttcgcctat tctttcacag attgatttat ctttaatgga
     2101 ctatctgcaa agaaaaatat cgtcagatat ttttgaaaat catatgtaca gtgatattcg
     2161 tatattggag atattggaag atttaggggt tgtatttttg gttccgcata cggttattat
     2221 aaaatccctg tattctgtct tatcctgttt ggaacaattc aaagaggacg aagaactgaa
     2281 tatgatacaa aatcttttag tgaatggtat tattcagcaa gtagtagcag agtaacaatt
     2341 ttgtttggtt atttgacagg caggtagcca aacaaatttt ttacagttta tatgactata
     2401 tatctataaa cgtcataaag ttaaaggagg aatgaataat gattgatgta aaaaaactgt
     2461 attttagcta tactgacaaa ccgtttgtgg agaatgtaag tttccatgtt gggagaggag
     2521 aaatttttgg ttttttagga ccctctggtg ctggaaaatc aactattcaa aaagttttaa
     2581 ctgggcttaa tacaagatat aaaggcagtg taaaagttgc aggtacagaa ataagggagc
     2641 atacaaatca gttttatgaa aatattgggg tagactttga attttcaacc tgctacgaga
     2701 aattcacagc acgacaaaat ctcgcctatt ttgcttctct atatgaaaag caacctcggt
     2761 ctattgatga attattgcat atggtaggct tggaaaatga cggggacaaa aaagttgctg
     2821 acttttccaa aggtatgcgt tctcgcttga atttcattaa agcgttagtc catgaccctg
     2881 atatattatt ccttgatgaa cctacaagcg gtcttgaccc tacaaatagt cgtttaatga
     2941 aagatattat tcttgcggaa aagaaacgcg gaaaaacgat tatcattact actcacaata
     3001 tgtttgacgc aacagagcta tgtgaccaag tagcctttat cgttgccgga aaagtcagtg
     3061 cattggatag cccgcataat ttgattatgt caaggggagc tgccaaaata caatatacct
     3121 actatgataa aggcgagaaa accggagaat gtctgcttga tagaaccaca gaagataagt
     3181 tgttaaaaag tttaatttcc gaaaaccgtt tgctttctat tcatagtagt gagcctacat
     3241 taaatgatat ttttgttgat attacaggga ggacacttca atgagattgg gacgtttaat
     3301 ttgtggtgat atacatttcc aatggaaata tggtttctat tttatctact ttatattaac
     3361 agttttatat gtgtgcggga ttgccgcttt gccggaacat tggaaaacgg atattgcttc
     3421 tattatgatc tactctgacc cggcagcaat gggattattt tttatgggag cgattgtcct
     3481 cttagaaaaa agtcaaaagg tgttaaatgc tatggtggta tcccctgtaa aaatatcgga
     3541 atatatactt tcaaaaacag ttgcgttgat tgctatttct acggtaatcg cattgatatt
     3601 aggggtagtt tccggcagca atcatttact gggtatcgca gttggtacgg ctttgacctc
     3661 agcaatcttt actatgcttg gaattattgc agctacaaaa atttcaaact tgaaccaatt
     3721 tctcattgtg attatgccga tagaaattat ttgctttgta ccacctattg ttggtctgtt
     3781 tgtgaagtta ccatatctgt tccgcttttt tccattcaca gcttgcatga accttataac
     3841 tggaaaaagt gttttactat cgtttgatat ggtacttgta attgctacgt tgattatcct
     3901 ctacatagtt gctcgacata cagttgagca tatgtggaaa agtttgggag gtgtaaagct
     3961 atgaaaaaga tattatccag caccatacag atatttaagc aaatcaaaag tgaccctatg
     4021 atgtttgcgg cttgcttctc cccttttatt atggggactt taattaagtt tggtattcca
     4081 tttcttgaaa gaataacgaa gttttcttta caagcgtatt atccgatttt tgatttatta
     4141 ctttctatca tggctcctgt attgctctgc tttgcatttg caatgattac attggaggaa
     4201 atcgacgata aagtgtcacg gtacttttcg attacccctc ttggtaagtc ggggtatctc
     4261 tttacaaggt tgggagtacc ctcaattatt tcggcggtca tcgcttttat tgtactgtta
     4321 cttttttcat tagaaaagtt gtccattgga atgacaatat gtttggcgct tctcggctca
     4381 gtacaggcaa ttattgtttc acttatgatt atcacattat caagcaataa attagagggt
     4441 atggcagtta caaaactagc tgcacttaca ttgctcggaa taccagcccc cttttttatt
     4501 gatagttact accagttcgc ggttggcttc cttccatcat tttgggtagc gaaagccatg
     4561 cagaatgaag cggttcttta tttctccata ggattggtgg tagctttagt ctggtactac
     4621 ttccttacaa aacgtctgtt tcggaagctg gcaggataag gaggcatatt atggaaaaag
     4681 gtagacttct gcaaaacaaa aatatgaata aggctattca ggtttccaaa attacactaa
     4741 tcatcaatct gagtttatcg ctacttaaat ttgctgccgg atatatagga aagtcaagtg
     4801 ccatgctgtc ggacgctgta cattcggctt ctgatgtagt aagcacaatc gttgtgatgg
     4861 ttggaattaa aatatcagaa aaacagcctg atagagaaca tccttatggg catgaacgta
     4921 tggaatgtgt ggcttccatt attctctctg ttgcattggc tataacgggt gctggaatag
     4981 gatattcagg tatcaagaaa atcttttcag gacagtacaa tacgctttct gtccctagtg
     5041 gaatcgcact tacggcagca gtcttatcta ttgttataaa agagtggatg tactggttta
     5101 caagaagtgc tgcaaagcat acaaattctg acgcacttat ggctgacgca tggcaccacc
     5161 gctctgatgc actctcgtcg gttggttcct taattggcat tttaggagcg cgtttaggct
     5221 atgctattct cgatcctatt gcaagcgttg tgatttgcgg ttgtatctta aaagctgctt
     5281 tgaacatttt caaagaaagc attaacaaaa tggtagacca tagttgtgac aatgctactg
     5341 aaacaaggat tagggaagtt gtgcttcagc agcaaggggt tgatggtatt gatgaattaa
     5401 aaacccgcat gtttggagcg aaaatgtatg tagatattga aatattagct gatggtaatt
     5461 tagctcttta tgacgctcat agaattgctg agggagtcca tcaaacgatt gaaaacaact
     5521 ttccacaatg taagcattgt atggtacatg ttaatacgaa tgaacttctc tattctgcat
     5581 aggacaggga agatgattta aggctattga aagggaaagg agggaagtag gttatggaac
     5641 aatactggtg gattatcatt gttgtggttt tgattgtcgt tggcttactt aaaattgtaa
     5701 ttatgaagaa gtataaccaa aggagaaatt ctaacaatcg ttcaaaagat agcgaggacg
     5761 acaagatgta ttaaggttta ttgaaatctg ttatgttgta gaatgatgtt tttagatact
     5821 agaaagtgct ttaagggggg ctaacgttaa ggaaagcttt gagtacaatt ctttacaatc
     5881 aacagtcttt tgcatagaga aatgtatgta ggaaatatgg tgcaaggcac aaaacaccag
     5941 caattgagaa atcaacctat gaaccaaagg cacactacat tttatactac ttgacaagta
     6001 aacgaacatt tactttaatg gtttaaagaa gtaaacgttc gtttactaaa gaaggaggtt
     6061 gtaaatgtct gacacaaaag aaaatatatt aaaaattgca ctacacttat ttgcagtgaa
     6121 tggttatgag gctgtttctg ttagtatgat tgcggaagcc ttgggaatga caaaaggagc
     6181 attatataaa cattataaaa ataagcaaga tatttttgac tgtatcgtag aacgtatgta
     6241 tcaaattgat aataagcgag ctaaggatta tcaagtacca gaaagcacaa tagaggtaac
     6301 cccaaatgca tacagaaata caactattgg aagtctaata acatttactc ttgcacaata
     6361 cgatttttgg gtgactgatg aatttgcgtc taattttcgc aaaatgttaa tactagagca
     6421 atatagaaat ttaaaaatga atatgcttta tcaaaaatgt cttgtaaatg gtcctatcag
     6481 ttatatagaa gatatattta atgaaatgat tgaaaaaggt gttcttcaag agtgtgatac
     6541 aaaacagctt gctattgaat attatgcgcc attatttcta ttgataagtg tagcagacgc
     6601 agttgaaaat tgttgggatt taaaaactca attaactaca cacgttaaac aatttgtcaa
     6661 gaaatatatt ataaatgaga aagtaaaata atcctttatt ctgtcctaaa tataaaccta
     6721 actttttaat ttcagcagag aaactcaaaa tgaatattgt tataagtcac aaatattaac
     6781 tattaaaata ttacaataac ggagatgaga aaatggaata tattcgcagt aaaaaatatt
     6841 ttacacccga cttacagaca aaaattatgg ggccgaatcc tataaaatta caagaggaac
     6901 tattacaaaa tcataaaatc ccgtctggta gctatgtctg tgaccttgga agtggacaag
     6961 gtttaacttc tgtatttctt gcaaaagaat atggatttgt tgtttttgca gttgacttat
     7021 ggagtgagcc aaaagaaaat gaagaatttt tcaaaaaaat gggattatct tctgcacaaa
     7081 ttaaatctgt aaaagctgac gccaatgaac ttaaatttga gaaagagttt tttgatgctg
     7141 tagtaagtac agattcatat aattattttg gacgtgaccc tatatattta gatgaaaaat
     7201 tgcttccatt tgttaagaaa ggtgggtttg tttatatcac agttcctggc atgaagaaag
     7261 attgccatga gaaacttcca gaagagttat tgttatcatg gacaccagaa gaacttgatt
     7321 atatacatga tataacatat tggtctaaca taattagtca ttgtaaaaag gcagatttaa
     7381 tttcaatata tgaaatggaa agtaattcgg aagtttggtc ggattggata aagcaaaata
     7441 atgaatatgc aataaatgac cgtaaggcaa tagaagctgg cggtggagat tatctgaatt
     7501 ttattgctat aattctacaa aaaaaataaa ttctttagaa ccaaacacta taatttgcgt
     7561 tctaatatgt ttaccgattg acatcaataa ttttatcttt gagggtaatg atttgaaaat
     7621 agaagaatac aagaagatac gaaattgata aattagaatt tgatgggatg gtgatattat
     7681 gaaaatatta tggatcatta ttattatggc agataatctg ctgccgttac tgcttgctcg
     7741 tttctatcca aactataagc ataaagaaat ggcactatct gtccttggct ctcgtcaaag
     7801 cccggtgaaa tggatatata atgtgtggtg cattatatca ggtgtggtat tttgtatcgc
     7861 tccatatgcg ttgtatcaag agaacgatag cggatgggca atcgctatat ggatgcttct
     7921 tgctatatac ggcatcgggt gtgaaatcat ttccggagtg tgcccgttaa atgaaaatag
     7981 acaagacgaa gatataatta caaaaattca cggcggcaca tcggcaatcg gatttatggc
     8041 cttgcttgtc gttcctttgc tctttgcaat tctgcaattc caaacaacaa aagtagtgtt
     8101 aggcgcaatt tcactggtgt gctttacaat agcattcata ttcttctgct tttttattat
     8161 gggtgagaag gaaaagtttg caaatactgt tttgagatat ggcggtcttt ggcaaaggct
     8221 tgttttaata agttgttata taccgttatt tgtttggtct gtttctaatc aataaaattt
     8281 agtttatagg aataacaaat tccagtttgt aaaagtcaat aaatccaaag ataaatttgt
     8341 atttgaaagg agttttgaag aatgaaaaaa tatgagtatg tagaaattaa ctataatgcg
     8401 aaaggagttg tattcctttg tactgataaa cacaaagaaa ttattgacag ttacgcaaaa
     8461 acggggtatc actacgcagg gtttgtaccc acagaaattg acgcaaaagg ctgtatgcga
     8521 aagatagatt taatttttga aaaagaagat taacttattt tagggagttg gttctaatga
     8581 taggacttaa aagacgagat atgaattata ttatgaaagt tggtgctaaa gctcaaggtg
     8641 tttctgtatc tgaatttagg gctatgctac aagataccat agacaaaact atggaaagta
     8701 cagacccaga cgtgcaggca aattttaaac gatactttgg aaataagcgc ccaacaccag
     8761 aggaatatat ttatacgatt accaaaaaga caaaggttaa ggtttaaatg ttgtaaaggg
     8821 ggcttgccgt atgaaacgct ttatgtacaa ttcagtatat tcaagcccaa tttaacagca
     8881 tactatttac aattaaatac gctataccag tagctcgtac aaaatttata ttttgtgcgg
     8941 gcttttttaa tacccgaaaa gtttttttaa ttattttcat ttttttggaa gcaaatcaca
     9001 ccttgctgta cagatatggt gcggaaagag ggataaacac ttttcaattc atagctggaa
     9061 aggaggtgag attatgaaac cgtctgactt ccaaaaaaca attcagtgtc agtttgaatg
     9121 taagctgaaa aaagttgtca aaggtattat ctggaactac cgtaaagaat taaaacgaag
     9181 aaaaaacaag gaaatatctt ttagcgaatt accagacatt acggtagaaa gtctggcaac
     9241 atgggacgac tacgaaacaa actacacaat ttttaatgtg tgtggcatag atattaaagt
     9301 ccttgatgat gaacttgcag aagctctcaa acagctaccc gaaaaaaaac gtaacaatgt
     9361 attgatgtat tacttcttgg aaatgagtga tacagagatt gcagatatta tgaattatac
     9421 cagaggggct gtgttcaaac acagacaccg tgcactagaa actatgaaag agatactaaa
     9481 ggagaagaaa tagaaatgaa gaaaaagtac aagcacccgt cttattattt gatttcatca
     9541 gctatggatg gggatgaaac tgcgattgag aaattactgg cattctatga cccgtacata
     9601 tcaaagtgtt gtttacgtcc tttatatgat gaatacggca atgtttatat tgttgttgat
     9661 atggaattaa agggacgtat tcgagaagcc ttaatcaaaa tgattttaga ctttgagatt
     9721 gatatagata tagaaaccga agaagaataa gcaaatacca gcagggcgtg attggttaaa
     9781 ttcccttctt tcctgccctg ctcctgcttt tatacatgga agcctacggc tttcgccgat
     9841 tgttctttga caactgaata aagcagacag atacgtttgt gatacagcga gccacgggga
     9901 ctgtacgcca tgacctttcc gacaagaaag cgagcgaccc acgcagttga tccgagagcg
     9961 acttgtggaa tggtcgtttt gccatgacct gctatcacag aataatgata cgtccgcatg
    10021 gtgcggttct gcccacaaga atgggaatag ttgagatact aacggagctt tccaaagccg
    10081 tctgtctgct tatgataaga cacacagaaa ggagttgttg caacatgaac aatactgatg
    10141 tgccgatttg ggaaaaatac acccttagta tagaagaagc gtcccgatat tttcgtatcg
    10201 gggaaaagaa gttgcgtaaa cttgccgagg aaaatctgga ctctggttgg gttatcgtta
    10261 atggaaaccg tattcagata aagcgaaaac agtttgaaaa aattattgat acactggacg
    10321 aaatctgatg tcttagagcc gtagatatgg tataatatta gtagcgtatc acggctcatt
    10381 ccatgacgga aaggagcttt acactatgtc aaaaatgaaa agacaagaca gtaaaggacg
    10441 caatttgcgt cttggagaga gccagagaaa agacggaaga tacgtttata aatataccga
    10501 tatattcggg aagccacagt ttgtatatgc ttggaaactt gtaccgacag acaaaacacc
    10561 tactggaaaa cgtgatgata aatcattgag ggaaaaagaa aagcagataa aaaaagacct
    10621 tgacgacggt atcgacacta tcggcggtaa aatgacagtc tgccagcttt atgcaaagaa
    10681 gaacaaccag aaaaagaaca tcaagagaaa cacggaaata ggacgacagt atcttatgaa
    10741 cgcactagaa aatgacccat taggtatgag ggcgattgat acagtcaaac agtccgacgc
    10801 taaagaatgg gctatcagaa tgaatgacaa aggttattcc tataagacaa tagataacta
    10861 taagcgttcc ttaaaggctt cattctatat ggcgatacaa gacgactgta tcagaaaaaa
    10921 tccttttcat ttcaagctaa gtgatgttct ggaagatgat acagagccta aagttatctt
    10981 gacaccagag caggaagaaa aacttctttc ctttatggaa acggataatg tttacagcaa
    11041 gtatcgtgat gaagttattc ttctgctgga aacgggactt cgtatctctg aattatgcgg
    11101 actgacaaca cacattgata tgcttaatcg tgtgattaac atagaccacc agctattacg
    11161 tgatacagag gtgggctact atatttccac acctaagaca aaaaacggca agcgagagct
    11221 tccattgaca gaaagagcat atcaggcact tgaacgaatt ttaaagaacc gaggaaaagc
    11281 tcaaccgctt gttgtagacg gttatagcaa tttcttgttc ttgaaccgtg agggcttgcc
    11341 aaaggtagca ggaaattatg aaagtatggt gcgtgggctg attaagaagt acaacaagac
    11401 ccatgaggac aagttgccga acatcacgcc ccactcattc agacatacct actgtacgaa
    11461 catggcaaat aagggaatga accctaacac cctgcaatac atcatgggac acgcaaacat
    11521 aaccatgaca ttagggtatt atgctcacgg tactttccag tctgcaaagg cagagttgga
    11581 aaggctggct tcttaatatc gaaaatgtat cttactactt atttactact tttggttgca
    11641 ttttcatgct ggtaaatgcc agcttatgcg aggtatcttc caaaaagaaa atgcccatac
    11701 agcccgaaaa tacgggatat atcggcattt accaacttat gaaaagataa tcagaaatta
    11761 gtaagttttt atatcaaaaa aatgattact gtaaagaaat cattttttat tataatcact
    11821 gatacatcaa atattctaaa taaaattttt atttatctgg aattgtatat tttttctata
    11881 atattaacta cagcgtacat tgcccaagca cagatagcca atacaaatac tcccatcatt
    11941 actaaatcta atttaaatac ttgagaacca tatactatca agtagcctat tccatatctt
    12001 gaaactaaaa attctccaac tattacacct acccatgcca taccaatatt aattttagtt
    12061 aaatttatta aatttcctgt gtttgcaggt aatatgagtt taaataatat ttgagattta
    12121 gtagctccaa aactttttaa catcttaatt ttttcttcat ctatattcat aaaataatta
    12181 taagctgaaa gtatagtaac aacaacagaa atcgttacag ctgttacgac gattccttct
    12241 attccagcac ctacccaaac tataattata ggagctaaag cagtcttagg aagagcattt
    12301 aaaactacca aaaatgggtc taatattttt gataattttt ctgaccacca aagagcaatt
    12361 gctaccaaaa tacctaatac agtacctata acaagaccaa gaacagtttc atatacagat
    12421 attccaatat gtttaaataa ttgaccacta aatgcatatt gaataaataa attgtaaata
    12481 tcacttggtt tgctaaatag aaatgtattt ataacattta agtcagctaa aagttcccag
    12541 attacaataa aaccaattaa aattaatagc tggtagaata atacatttct tttttctctt
    12601 tttacacctt taacatagtt tatatatcct tcagatcttt tattaagcct catgtgtatc
    12661 tagctccttc cataatatgt cgaaatactt ttgaaaatca ggaactttac gtgttttgag
    12721 aggcgttttt gttccatcca tgttaagttc aatatcgtaa atatttttta tggtagctgg
    12781 tcttttagat agtattgcaa ccttatctga tatgcttatt gcttctgcta tagctaaaaa
    12841 agtgcaacta attgcttaaa gaaacagaat tatcaacttc agcacccata taaacaatat
    12901 taagatttcc taaactgcta tcccaaacga tactattaat aatagtagat aataacaatc
    12961 tcttatcatc taaactggca ttatcaactt catcattaaa tctcttcaaa ttactaacaa
    13021 taatatcaat attaatactg tcactactaa tattgatgtt actagattcc aaaatattaa
    13081 tcttatcctt taaaatacta atctcattat ttaggctctc aatctgggaa actatataat
    13141 cagaagcagt actaccagta ttttttgcaa gctctataac taaattttta ataagtcttt
    13201 ccttatctaa aatttgattt ttaagtaaat tagtctcaga tttaaaaata ttaaaaacat
    13261 tatcagtctt aatgttatta ctattgaatg cacttataat agactcttct ttacaattct
    13321 taattttatc tataactaaa ctttcaacta atggaccatt aagattttta cagttgcaac
    13381 tatcagaccc aagtgttttt ttagtaccac aaatataata atactttaaa tccttgtcag
    13441 tttttttgcc tttataggta attctcatat tagaaccaca tttaccacat tttataagac
    13501 cagataaaag agcagttttt ccagttgata tccttggagt tttatcacga tttttatcta
    13561 attgattttg tacttcaagc catatttcag gttctatgac tccatggtgt tttgcaattg
    13621 ctgctattga gttagatgta tttttagcat aggtaaggat tccacatttt ctatctggag
    13681 tacctacaac atccatacca ctatttttaa ggtaatctaa aatttcatca ttagctttta
    13741 catatgctgg atttctaagt attctagata aagaactagg gtcaaatttg ccaccacgag
    13801 gaccccttat tccattatta tagacatatt tatataactt agataaggac ctaagttcta
    13861 aatatttatt gtatattagc tttacaatct ccatttgttc atcatttact tgaagcttat
    13921 acatacttct ttcttttaga ttttcatcta agaatgtgat tttttcactg ttgaatccat
    13981 gaggtgtatt gcctccaagc catctaccac tacgggctaa ttcatacata ttgtctttta
    14041 tacgttctgc tattgtttct ctttctaatt gagcaaatac acttgatata aacatcatag
    14101 ctcttcccat tggagttgaa gtatcaaatt gttctttgat agatataaat gatatgctta
    14161 gttcatttaa tttttctatt aaagtagaga agtcagatac atttctcgat atcctatcta
    14221 atctataaca aactaggtaa ctaaatttct tatctttggc atcctgcatc attttcttaa
    14281 attgtggtct atctatattg ctaccactaa atccctcatc ttcatatatc aaaaaatctt
    14341 ttacaccaat agactttgca taatcaatac ataattgtat ttgattttct atagaatcac
    14401 cttttccagt aaatcttgat tttcttgaat aaattgcagc tttcaaaatt aaaaccccct
    14461 ataagttagg atatatattt gaaactattt atctacaacc ttttaagtaa acttattaga
    14521 atgaatttat taattaggta aatttatata attctacttt ttataatata tgactattta
    14581 ttttatttct ttactattca agttctaaat caataataaa ttttgatatt taattgtatc
    14641 aggacaaatc tctaatttca tagattatta atataaaatc ataggttagt gtgaaaaagt
    14701 tgaaatttaa atattttaaa agataatttt tttgaactaa taggtataac aaaaatttat
    14761 agggggattg catatggaaa tcaatgttat agaaattttc cctaaagata aagctaaact
    14821 taataaaata gaaatggata aagctagttg gtttgtaaat ataataagta aaaaatatcc
    14881 taaagaagct ttaaatgaag catttagtac tttagaaaaa gaattaaata taagtaaagc
    14941 taatacataa gctttgatta ttgattttat atactattgg ttttatacgc tatgacttga
    15001 cattatatta atcaaatagt atatttttaa atatagcaca atgaagtgtt aaaagagcaa
    15061 tggagctcca tagataaagt ctatggagct tttttgcgta tttaacttaa ggaggatagg
    15121 actttgaata actttgagat taaaactaga ataaaatttg gagaaggctc actaaaagct
    15181 ttgcaagaat taaaaaacaa aaatgtacta ataataacag acccttttat ggtgaaatca
    15241 aaaacaatag ataaaataac atctaattta ataaactcaa catatcaagt ttttgaagaa
    15301 atagtaccag acccaccaat agaagtagta gtagcaggaa tagaagcatt taaatctata
    15361 aatccagacg taataatagc attaggtggt ggttctgcaa tagatgcagc aaaatctata
    15421 atggactttt ctaaaaaaat attaaaaatt aacgaagtag agtttatagc aataccaaca
    15481 acaagtggaa caggttcaga agtaacatca tttgctgtaa taacagatag tcaaaaaaaa
    15541 tgtaagtatc cattagtgag tgatgattta cttccagata tggcaatact agacccagaa
    15601 cttgtaaaaa ctgtaccaaa ttttataaca gcagatacag ggatggatgt attgacacat
    15661 gctatagaag cttacgtatc tacaaattct aatgactttt cagatgcact agctgaaaaa
    15721 gctataaagt tagtatttaa atacttaata aaagcatata aagatggaaa tgacatagaa
    15781 gctagagaaa aaatgcacaa tgcatcttgt ttagcaggta tggcatttaa ccaagcatca
    15841 cttggcataa atcattctat tgcacatgta ttaggtggaa aattccatat accacatgga
    15901 agaacaaact ctatattact tcctcatgta atagaatata attcatcaat agtaggatat
    15961 actaacacaa cttactctgc tacagctaaa aaatatgcac aaatagcaga attagtagga
    16021 cttgggagtg ctaatataag aatatctgtt agaaatttag taaatgaaat aaagaaacta
    16081 caaaaagaaa tgaatatgcc aactaaattg acacaatgca aattaagttt agaagatata
    16141 actaatgaag aaagagaaat agcaaaactt gctataaaag atacttgtac attaactaat
    16201 cctaaggtac caactgaaga tgatataata aacatattaa agaacattaa ataactacat
    16261 tttattacat gtaaatataa tattatacaa atagggaaaa tgtttttaaa aatataaatt
    16321 atatattgac aaatataaaa cataaaacta taatgaaagt atcacaaagg cgtgatactt
    16381 tagtagaaag caatggagct tcttaggaat attaatatta ttcttatgga gctcctttta
    16441 ttttaatcaa atagcttttt aataaactat attcaaagaa agcagggaat aaatttgact
    16501 gaagaatcta agcaaagagt tattcaagaa tatgttcctg gaaaacaggt tacattagca
    16561 catatcatag ctaaccctaa tgaagatata tataagaagt taggattagt acttgataaa
    16621 aaagatgcaa taggaatact tacaataaca ccaagtgaag cgtcaataat agcagcagat
    16681 gttgctacaa aggcttcaaa tgtatcttta ggttttatag atagatttag tggttcagta
    16741 gtaatatcag gtgatgtaag ttctgtagaa tcagcgttaa atgatgtttt agaagtctta
    16801 ggaaatatgt taaacttctc atctacaaaa atcacaagga cataataata caatgaagaa
    16861 tataatattt atgggaaaga caggtagtgg taaaactacc ttatgtcaaa aactagacca
    16921 gttagaatta aagtataaaa aaactcaatc agtggaactt tataataatg caatagacac
    16981 tcctggtgag tatatggaaa atagaaacct atataatgcc ttaataacaa catcagcaga
    17041 tgctaaaatt atagcaatag tgtatgatgc cacacaagaa gaaaattata tagcaccagg
    17101 ttttgccagt atgttttgca aagacgtaat aggaattata actaaaataa ataaagctac
    17161 tgaaaacgaa gtcaatatag cttatgaaag actatcacta gcaggagcaa gtcaaatctt
    17221 taaaatagac acagttgata atattggact tgaaaaatta tttaaatatc tagaaaaata
    17281 taaaaatata gatgaagttt aaggggaaat atttttttat tcaaaaatat tattaaagta
    17341 tgataaaata aaaaaatgta taaactattt taagaagatt taatctttaa gcgaggtagg
    17401 aacatgggga aaaaaatact attggttgat gatgaaccac tgttaaggat ggatacaaaa
    17461 gatatattag aaagtcatgg ctatgaaata ataggcgaag caagtgatgg atttgaagct
    17521 gttgaaatgt gtaaaaaaca caaacctgat ttagttataa tggatataga gatgccaata
    17581 cttgatggta taaaggcagg caaaataata tgtaaagaaa atatagctgg tggagtactt
    17641 ttattaactt catttgaaga taaagaatac gtagaaaagg ctaaaagtat aggtgcttat
    17701 ggatatttag taaaaacagc aagtgaaaaa tcacttatcc caaccataga aatgtgttta
    17761 aataaggtaa atgaatttga aaaaatgaaa aaagatttag aaaaagtaaa ttctaaattg
    17821 aatgaaagaa aattagtaga aagagcaaaa ggtatactta taagagagtt taagatttct
    17881 gaggatgaag catacactag aataagaaaa ttaagtatgg ataaaagaac tacaatgatg
    17941 gaaattgcca aaatgattat aataggatac gatgactaat acgataaaaa aaatatcaac
    18001 tgcacaaaca tacttaaata ataacgaaat tgaaaaaatt atagaagtat caaaatcctt
    18061 agatttaatg gcttcttttt atgaagctga tgtttttata gatgtactaa gtaaatttga
    18121 tgatgaagca attgttgtgg cacatggtag acctaaagaa aagtcaatgt actgtgaaaa
    18181 tgttataggt aaaaaggctt taattgaaaa tgaaccagga gttataaagt gcttaaaaac
    18241 aggagaaaga gtaagagata ttaaagcttt aacacaggaa tataaactgg tcaaacaaaa
    18301 tattcagcca ataacactag atgacaaagt tttagcagta ctaatagttg aaaaagatat
    18361 aagtagtgaa gttaagaatg agtttaatgc tagtgaagac gaagaaaata aaaatttaca
    18421 tcagtttata aaacttattg gaaataatga ctttttagtt gataatttaa attcagccat
    18481 attaatattt gataaaaatg gaaaattaaa aattaaaaat cgtattgcaa ctgagatata
    18541 caaaaatctt gggtttggat atgatataca agatatgcat tacaatgatt tagatataga
    18601 aaccaacacc tttgaaaata tattaatgga tgaaaattat gatgagacaa atgaagtttc
    18661 aataggtaat atgtttttta aaataaagac catatacatc aaagatgatg aatttaaggt
    18721 ttgcaaaata gtacaagata taagtgattt aaagagaaaa gaagaagaat tagttttgaa
    18781 aacagtggct ataagagaag ctcatcatag agtaaaaaat aatcttcata cagtaatatc
    18841 tataataaaa aaacaaagta gattatctca aaatgatgaa gtaaagcagt gtttagataa
    18901 cataataaat agggtatttg caatactttc gactcattat ttactatcta aagaagttga
    18961 taataatata tctatacgag aaggtatcaa tttattagtg tcaaatattc aaggtggcta
    19021 tttatttagt aaggatatag atatacgtat aactggtgaa gattttaaaa ttagtggaga
    19081 aaaagcaaca gcaatacttt tggttataaa tgaaatacta caaaattgtt atgaccatgc
    19141 attttcggat agagaacatg gtactataca aatattaata aataaagata acgactatag
    19201 aaatattgct attatagatg atggaattgg atttgatagt aataatataa ataaaaatag
    19261 cttagggaca tatataatag atagttatat aacacagatg ttaaaaggaa atatagagag
    19321 aaaaagtagt gagaatggta cagaagtatt aattactata cctacacaaa ttaatattta
    19381 aataataact catctacaaa gtagtagttt taattgagca aagaggctta aggttattaa
    19441 tagattaagt taattgaatg tatttataaa aattataagt atatattcaa tttaacaggt
    19501 atttattata actttaagcc tctttgcttg tttaagacat acttaaattt taggaggtga
    19561 gaaaatgaga gaagagttat taagtgttgg tattgacatt ggaacaagta ctactcagtt
    19621 aattttttct aaattaatag tagaaaatac tgaatcaaat ttttcggtac ctagaataag
    19681 tatagtagac aaagaaatta tttataaaag caaagtttat ttcacccctc ttatttcaaa
    19741 taatgaaata aatggtcaac aaataaaaga tatagttgaa aatgaatata aaaaagcaaa
    19801 tatatctaaa gaagatatac atacaggagc agtaataata actggtgaaa cagctagaaa
    19861 agaaaatgca aatgatgtac ttcatacttt gagtggattt gctggggact tcgttgttgc
    19921 aacagcagga cctgatttag aaagtataat atcaggaaaa ggagctggag ctcatattta
    19981 ttcaaaagaa cattcaacta caattgtaaa tatagacata ggtgggggaa ctagtaacat
    20041 agcctcattt aaaaggggag aagtaaccga tactgggtgt ttggatatag gtggaaggct
    20101 tgtaaagata gataaaataa gtaaaaaaat aacttatata tcacctaaaa tagataaaat
    20161 cataaaagat aataatttaa atcttcaagt aggtacaatg gctactgtag aaaatacaaa
    20221 gccaatagtg gatataatgg ttgacttatt aatggaaagc ataggactaa agaaacagac
    20281 acctttgttt gattttataa taaccaataa atcaataagt atagatgaaa atattaaaaa
    20341 tgtttcattc tctggtggag tagctgatta tatttactat gatggagaaa taaaagatta
    20401 ttttgaatat ggtgacttgg gaattttact tgggcaagca ataaaaaatt gtgaatacct
    20461 taaaaaagta aatgtagtta aatctatgga aacaataaga gccacagtgg ttggagcagg
    20521 ttctcatact actgaagtaa gtggaagtac aataacttac actagcgaaa agtttccact
    20581 taagaacttg ccagttttaa aattatcaat tgatgaagaa acaggtactt caaaacaaat
    20641 cagtgaagct ataaaaaata aacttaattg gtttaaacta gaaaatgatt tacagaaagt
    20701 tgctatagcc atgataggaa agaaaaatcc aaccttttta gatatacaag agtatgctaa
    20761 agggtttgta gatggaatgc aacaaatcat agaaaatgat ttacctttta tagtaattgt
    20821 tgaaaatgat atggcaaaag tgttaggtca agcaatatat gcattattaa attatgaaaa
    20881 agaaattgtt tgtatagaca gtgtcaaggt tgaaaatgga gactatatcg acattggaaa
    20941 acctatagtt gaaggtaaag ttttacctgt ggtaataaaa acattagttt ttaattaata
    21001 gaaggaggta agtaaatgat tttaaataca aaattatttg gacgtactta tcagtttaaa
    21061 ggcttatatg atgtaatggc taaagctaat gaagaaaaat caggagataa attagcaggt
    21121 cttgcagcag aatcagctga agaaagaata gcagcaaaag ttgttttatc acacataaaa
    21181 ttggaggata taagaaataa tcctgcagtg gcatatgaag aagatgaagt aacaagaata
    21241 atacaagatg ctgtaaatga aaaagtttat gaagaaataa aaaattggac agtgtctgaa
    21301 cttagagaat ggctattgga ttcaaatact acaagtcttg atataagaag aatatcaaga
    21361 ggattaactt ctgaaatgat agcagcagtt gcaaaattaa tgtcaaatat ggacttagtt
    21421 tatgcagcta aaaagataaa ggttactgct cactgtaata caactatagg agaacaagga
    21481 acattatcag caagacttca acctaatcat ccaacagacg acccagatgg aatacttgca
    21541 tcacttcttg aaggtttaac ttttggtata ggagatgcag tacttggatt aaatccagtt
    21601 gatgactctg ttgagagtgt tactaaagta ttaaaaagat ttgaagaagt aaaacaaaaa
    21661 tatcaaatac caactcaaac atgtgtattg gcacacgtta ctacacaaat ggaagcaata
    21721 agaaatggtg ctcctgcaga tttaatattc caatctatag caggttctga aaaaggaaat
    21781 gaagcatttg gatttaatgg cgacactata gaagaagcta gacagttagc cttaaaacac
    21841 ggtacagcta caggacctaa cgttatgtat tttgaaactg gacaaggttc agaattatct
    21901 tcagaagcac atcatggaat agaccaagtt actatggaag ctagatgcta tggatttgct
    21961 aaaaagtatg aaccattctt agtaaataca gttgttgggt ttataggacc tgagtactta
    22021 tatgatgcta gagaagttgt aagagcagga cttgaagacc actttatggg taaactacat
    22081 ggcttaccaa tgggtgttga tgtttgttac actaaccata tgaaggctga ccaaaatgat
    22141 atggaaaact tagccatact attaacaaca gcaggatgta cgtattttat gggaatacct
    22201 catggagatg atgttatgct taactatcaa acaacaggat accatgaaac agcatcactt
    22261 agagaaatgt ttggactaac agcaataaaa gaatttgatg cttggatgga aaagatggga
    22321 ttctctaaag atggtaagtt aacagagttg gcgggagacg catctgtatt attaggatag
    22381 gaggtattta atatgaatga gaaggattta aaagcattgg tagaacaatt agttggacaa
    22441 atgcttggag aattagatac taatgttgtg tctgagactg ttaaaaaggc aactgaagtt
    22501 gtagtagaca acaatgcttg tatagatgat ataacagaag ttgatataag aaagcaatta
    22561 ctagtaaaaa atcctaagga tgcagaagct tatttagata tgaaagcaaa aacacctgca
    22621 agattaggta taggaagagc tggtactaga tataaaacag aaacagtttt aagatttaga
    22681 gcagaccatg ctgctgcaca agatgctgta ttctcttatg tagatgaaga atttataaaa
    22741 gaaaataata tgtttgcagt ggaaacttta tgtaaagata aagatgaata cttaacaaga
    22801 ccagatttgg gaagaaaatt ttctccagaa actataaata atataaaatc taaatttggt
    22861 acaaatcaaa aggttttaat attagttgga gatgggctta gctcagctgc aatagaggca
    22921 aatttaaaag actgtgtgcc agctataaaa cagggtctaa aaatgtatgg tatagattca
    22981 agtgaaattt tatttgttaa acattgtaga gttggtgcta tggaccactt aggtgaagaa
    23041 ttaggttgtg aagttatatg tatgttggtt ggagaaagac caggattagt tactgctgaa
    23101 tcaatgtcag catatatagc ttataagcca tatataggaa tggcagaagc aaaaagaaca
    23161 gttatatcaa acattcacaa aggtggaaca actgcagttg aagcaggtgc tcatatagct
    23221 gaacttataa aaactatgtt agataaaaaa gcttcaggaa tagaccttaa ataaagtcga
    23281 gggagtgtag aatatgaaaa atgatgtaat tcgtcctaat atactaggaa ttaaaataat
    23341 ttcaaatata agtccagaaa tggcacaaaa actagaatta aaatcacatc acaaaagctt
    23401 aggtttaata actgcagact gtgatgatgt tacgtataca gctcttgacg aagcaactaa
    23461 agcttcagaa gttgatgttg tatatgcaaa atcaatgtat gcaggtgctg caaatgcttc
    23521 aacaaaactt gctggtgaag tattaggaat aatagcaggt ccaagcccag cagaagtaag
    23581 aagtggtcta aatgcagtag ttgattttct agagtatgga gcaactttta taagtgcaaa
    23641 tgatgatgat tcaatagcat actatgctca ctgcgtatca agaacaggta catacctttc
    23701 agaggtagca ggaataagag aaggtgaagc attagcttat ttagtagcac caccacttga
    23761 agcaatgtat gcactagatg cagcaatgaa agctgcagat gttaagatgt gtgaattatt
    23821 tgcacctcca actgaaacaa actttggtgg agcattactt acaggttctc aatcggcttg
    23881 taaagcagca tgtgatgcat ttgcagaagc agtaaaatca gttgcagaca atccaacagg
    23941 attttaagga tagttagatg gatgctatag gcttaataga agtaatagga tatgttgctg
    24001 caatagaagc ttctgatgct tgtttaaaat ctgcaaatgt taatattgta agagttgata
    24061 aggttggggc aggtattgtt actttaacaa tatgtggaga tgtcggtgct gtaaaagctg
    24121 ctttagaagc gggtgaaata gcagcttcca gagttggtac attaagaaca tcacatatta
    24181 taccgagaat ccataatgaa gtaacaaatg ctttattcaa aactaaagaa cctaaagtct
    24241 gtgaagtttc agaagataaa gaaaaaaact ttgagattac attggaaata gctgataatg
    24301 tatcgaaaaa tttatcagaa aaagaaaatg taattttaga tgtcaatgaa aacagtggag
    24361 atactgaaaa tttatatagt gatatagata aaacaaacaa aaaaattgaa acagttgatg
    24421 atagtttaac agaagctaat gaggaaaaaa tagaaaacac taatgataaa gaagtattgg
    24481 aacaagttaa aatagaaaat tccgattttg atagagatga cctatcaaag aacagtgtaa
    24541 aaaaacttaa agctatggct aaaaaattag attcttcaat aacttataaa gacttaaatg
    24601 ttcttaaaaa agaagaatta ataaacttag ttaaaaagct tagcagaagg gatagatagt
    24661 tatgacactt ttagataaag atttagtatc tatacaagaa gtaagaactc ttgtaagaag
    24721 atctaaagtg gctcaagaga aactttctaa aatgagtcaa gagcaaatag ataaaatagt
    24781 aaaagctgtt gtagatgcag catttaataa tgctactaaa ttagctaaaa tggcaaatga
    24841 agaaactgga tttggaaaat gggaagataa agtaattaaa aatacttttg catctatggg
    24901 agtttatgaa actttaaaga acacaaaaac tattggaata attggtgaag ataaagaagc
    24961 caatacgata gatatagcta caccagttgg agttgttgca ggtcttatac cttcaacaaa
    25021 cccaacatca acagtaatat acaaaactat aatatctcta aaagcaggaa atactatagt
    25081 tttctctcca catccaaatg caaaaaactg catacttgaa acagttaata taataagtga
    25141 ggctgctata aaggctggag ctccagagga ttcaatatta tgtataacta atccaagtat
    25201 agttggaacc aatgaattga tgaaacataa agatacaaac ttaatactag caactggagg
    25261 agaagcaatg gttagagctg cttactcttc aggaacacca gctattggag ttggaccagg
    25321 aaatggacca gcatttatag ataaatcagc aaatgttaaa ttagcaatca aaagaataat
    25381 agattctaaa acttttgata atggtgttat atgtgcatca gaacaatcta tagtaataga
    25441 aaaatctatg gaagaaactg ttttaaaaga gcttaaaaat caaggtgcat atgttttaaa
    25501 tgaagaggaa agtgaaagat tagcaaaatt catattaaga gctaatggta ctatgaatcc
    25561 acaaatagtt ggtaaaagtg ttgaggtaat agcaaattta gcagacttaa caaatgtacc
    25621 taaaaataca aaagttctag ttgcaagaga aaataaagtt ggtcacaatg tagcctattc
    25681 aagagaaaaa cttacaccaa tactagcgtt ctatgttgaa gaaaatgtag aagctattat
    25741 gaacagatgc agagaaatat tgttaaatga aggtgcaggt catacattct caatgcatgt
    25801 aaatgatgaa gaattggtaa aaagatttgc ccttaatatg ccagtatcaa gaatacttgt
    25861 gaatactcct tcagcacttg gaggaatagg tgcatcaaca aaccttgtac cagcactaac
    25921 acttggttgt ggtgcaatag gtggaagttc aacttcaaac aatataggac cacttgattt
    25981 aataaatgtt aaaagagtag cttatggaat caaagaaata gaagaacttg gaagagataa
    26041 atctcaagtt gaaaaatcaa tgtttgactc aatggataaa gaagaattaa taaatcttct
    26101 tgttcaaaaa ctagtcaaag aactagcata actaaaataa acaatgaaac ttatggctat
    26161 cgctataaat ttctaaaaat ataaactata aaaaactata aaattaaatt tttggaggat
    26221 aagattatgg caagtgcaaa cgcattagga atgatagaaa ctaaaggatt agtaggagca
    26281 atagaagcag cagatgcaat ggttaaagct gcaaatgtac aattagtagg aaaagagcaa
    26341 gtaggtggag gattagttac tgtaatggta agaggagatg ttggagctgt taaagctgct
    26401 acagatgcag gagctacagc tgccgagaga gttggagaac taatatcagt tcatgttata
    26461 ccaagaccac actttgaagt agatgctata ttaccaaagg tatcagctga gtaatataag
    26521 aataattata aaatttaaat tttgattgaa aaagaggcta tctcaaaaat gagtcttact
    26581 aataaactca atgtattttg aggtagtctt ttttatacat ctaataggag atgtgataaa
    26641 atgggtgttt taacagaaaa cgaagtaaaa agaaaaattg gaaaaaacaa aaataatata
    26701 actgaatttt atatagaaaa aggtcaaata ataactcctt ctgcaaaatc ttatttagct
    26761 gagagaagta ttgagcttaa atatgttgat aaactttcag atgtaaacaa tgaacctaaa
    26821 gaagttgtaa aagaagtagt taaagagaaa aaatataaat atactactgt atttggcgcc
    26881 aaattggatg aaaaaccaga acatatgact caattaaatg gaaatctttt agtttttaaa
    26941 gaccataaaa gaataatatt gagaggaaaa atagattctc ttgaatcaga aatactaaag
    27001 gtacagatac tttgtcagaa actagatatg ccaaagcttg ttaaggaatt gcaagaagta
    27061 ttagactttg taagaaatat aatgagatgt gaagttttag atgaaaagct aggggacttt
    27121 aaacttttag gattaactcc tcaagaactt agagaacaat ctcactatcc taaaaaatac
    27181 tttggtatag gacatgagtt cccaagttat gaaatgggag aagtagtagt agctataaat
    27241 agtataagaa gtaatacacg agaggttgaa cttgcagcct atgaagcatt taagggtgag
    27301 tatggaaatg ttcacagaga agatttaata aaggcactta atagattatc gtcagtattt
    27361 tggataatga tatataaggt tagaactaat aagtataagt agttggagga cataatggag
    27421 aatctattaa atgaaatagt ggaagaagta ataactagag ttaaaaaaga agcatttata
    27481 gaagttgaag catcaggaag acacgttcac ttaagtagag aagatgtcga taaattattt
    27541 ggagaaggat atactcttac aaaactaaaa gacctatctc aaccaggtca atatgcatgt
    27601 aaggaaagag ttacaataac tggtcctaaa ggaagtataa aaaatgtagt tgtacttgga
    27661 ccatgtagaa atgaaactca ggtagaaata tctttaacag atggttcaac tcttggacta
    27721 aaagctccta taaaacaaag tggagactta gaaggtactt taagtattaa aatatcaaca
    27781 caatatggtg aagttgaact tgataagggg ctaatggttg ctaaaagaca tatacacatg
    27841 actcctaaag atgctgaaaa attcaatgta tgtgataaag agatagttga agctaaggtg
    27901 atgggtcaaa gacctttaac atttgatgat gtagtaataa gagtgagtga cagttttaaa
    27961 acttatatgc atatagacta tgatgaagcc aatgcatgtg gttattcaaa aggaactgtt
    28021 gctaaaataa taaagaaagc ataaatttaa cctactatat aaattacctc tagggaggat
    28081 ttaatatgaa ttatgataac cttgtagatt taataacgga agaaatctat aaaaaaataa
    28141 atagtaatga aataaaaata tctaataaac ctaaagctgt gattgtattt gaacaagata
    28201 acaataaatt taacttatta aaagatggat ttgaaatagt agaatttgat aaaagtataa
    28261 gggaatgtga aatagtgata gtttcaagac tttgtatgag ggggctatcg aatatagcac
    28321 ttggaaattc aacaagtgat gaagaaagat ttatactaaa aatgattatg aatggcaaaa
    28381 aagtatatgt cctagatgaa ggaattgaat acaaaaaata taaggatact gcaccaaaag
    28441 cactatataa gaaatttatg tcatttgaag atgaaatatg taaatttggt gtagaaatta
    28501 taaaagactt aaatgttata actaaaaata agttaaatat aaagaaagaa ctagaaagta
    28561 ctagaggttc taaaaaagaa gatacaaaag ttttagataa agatatttta aatttaacca
    28621 gtgtagaaag tgtaaaaata gataatgaat ttagtttgga tttaaggaat aaaaaactta
    28681 tatcggaagc tgacttaaga aaaccaacta taaatggagt taaaaacata ttagttaata
    28741 aaaaaagtat aataacacca ttagctgtag attttatgag aatacaccat ctcaaactaa
    28801 agaaactgta aggaggtaat tttatggata taggaaaagt tgtaggtaat gtttgggcta
    28861 caagaaaaga tgaaaaatta tgtggccaaa aacttttagt tgtaaaaatt ttaaaaacta
    28921 aagacacata caaggaaggc ttcatggttg cagccgataa tgcaggagca ggaaatggag
    28981 acttagttct tatatccaaa ggttcctcag ctagagagtc gattgaaaaa tctcatgttc
    29041 cagtagatgc aactatagtt ggagtagtcg attcgtttga ggtgtttgat gaataaaagt
    29101 ataggagcta tagagtttaa aagcattgct aaaggtatag aagtatccaa tgaaatgata
    29161 aagaaatctt cagttgatgt attgtactta aaaagtattt gcccaggtaa atttctaata
    29221 atagtaggtg gagaaacttc ttatataaat gaatgtgtag attatggaat aaaacttgga
    29281 gaaggatata ttgtagataa cttcgtaata aatgcaattt cacaagaaat attagatgga
    29341 tttaaaaata agtatcaaaa gctagatagt atagtctcta taggcgttgt tgaaagtagt
    29401 aaaatatgcg ctggaataaa aatgcttgat aagactttaa aatctggcga cttagtatta
    29461 gtaaaacttc aattatcttt tgctataggt ggcaaattag tatatatagt agcaggagat
    29521 ttaagtagtt tagaatatgc attaaaagaa agtgaaaatg tagtaagaga aaaagaagta
    29581 atatataaga ctgtaatacc atcagtagac agtcaaataa taaaaagtct aataaaataa
    29641 tggggggaat tatgagtata aacgaaatta taatctatgt aatggtagtc ttcatggtac
    29701 ttggagctat agataaatgt ataggtaaca aatttggtct tggagaacaa tttgaggaag
    29761 gtataatggc aatggggtca cttgcagttg caatggttgg tgttatatgt ttagcaccag
    29821 ttcttgctga tgtattaaga ccaattgtag taccagtatt taatatactt ggagctgacc
    29881 cagctatgtt tgctggaagt ttattagcta atgatatggg tggtgcgcca cttgcacttg
    29941 aacttgcgaa agaccctaat gctgggttat ttggtggatt aatagtaggg gctatgatgg
    30001 gacctacaat agtatttata ataccagttg cgcttggaat aattgaaaaa gaagaccaaa
    30061 aattcttagc aacaggaata cttgcaggta taataacaat acctattgga gcattcgttg
    30121 gtggattagt tgcaggattc gaggtaatga tggttcttag aaacttagtg ccaataataa
    30181 tatttgcagt agttatagca cttggattat taaaattcga aaatgctatg ataaaaggat
    30241 tcacatactt tggaaaaggt gtagtaatag ttataacttt aggacttgct gctgccatag
    30301 ttgaagcttt aacaggaata gttgtaatac ctggaatgaa accaatcagt gaaggtatag
    30361 aaatagttgg agatatagca atagttttag caggagcatt cccattagtg tttgttataa
    30421 caaaggtatt taataaacca ttaatggctt taggaaaagt tcttggaatg aatgatgtat
    30481 cagcagcagg ccttgttgca agtttagcta actgtatacc aatgtttggt atgatgaaag
    30541 atatggacaa tagaggaaaa ataataaatg tagcattctc tgtatcagca gcattcgtat
    30601 ttggagacca cttaggattt acggcaggat ttaattctaa tatgataaca ccaatgatag
    30661 ttgctaaatt agttggtggt ataacagcag tattgttagc tatggttatc gcaaataaga
    30721 cactaaagaa ggagaatgta taagatggat atatcaaata tagataaaaa tttaattgaa
    30781 acattagtaa gacaaataat agaagaaaaa ataagtggaa ctaaagacac agtagatttt
    30841 gttagaaata aagacattag tggaataact tctataaagt taccaactgt aaaagttagt
    30901 gaatctgata gattagatac agggaatcca agtgatgtag tttacacaaa agatttattt
    30961 acattagagg aaagcccaag attaggttgt ggtatgatgg aaatgaaaga aacaactttt
    31021 gattggactc taaattatga tgaaatagat tatgtaatag atggaacttt agatataata
    31081 atagatggaa gaaaggtttc tgcttcttca ggtgaattaa tattcatacc aaaaggaagt
    31141 aagatacaat tttctgtacc tgattatgct agatttatat atgtaacata tccggcagat
    31201 tgggcatctc aaaattaata aaaatcaagt taagttaata atcaaataaa taatttaaaa
    31261 tacaaactct taaataagca cgcatattta aaaaagctat gaattaaaat tcatagcttt
    31321 ttttataaag aatgttatat tttagacgta ttttttctat atttactggg agatatatta
    31381 tattttttat taaataatct aataaaatgc tctacatttg gataaccaat actattttac
    31441 tgataatctt ttccttatca ctaatcttac attttaaaat attattttta ctcgtaatat
    31501 tatcataaga atttgtttct tatttaaaac taattagaat tataaagttt attgccatgt
    31561 tattaataaa ataaatccag ttaaatcgag tgctagttaa agtaataaga aacttttaca
    31621 atggtttcct ttatcgattg aaataaaagt taatatggta taatatctaa agagagaatc
    31681 ttatcaatta gaatatgcat agaaacaagt ggcttaaaag gagacgttat atggaagaga
    31741 tagtaaagaa ggaacaagca agtatgaatg atattttttt gtatcttaaa gctaaatcaa
    31801 actcattata taaatttgta atattatata atgagtttat caataaacct aaagattatg
    31861 gaactgggga acttataaac atggttgaag tacatatgct aacagcgatt gaagaaaatc
    31921 caggaataag tccaactcag ttagctaaaa tatggaacag aactaaaggt gcaatatctc
    31981 aaactatatc taaattggag aaaaagggtt atataactaa agaaaagaaa gaaggaaatg
    32041 caaaaactat attactatat ccaaccacat caggagtaga gttaagtaag gcacataaac
    32101 tatatgacac catagatgtt acaagaacat ttgaatactt aatcaaggag tgtacattag
    32161 aggagataga tagtttttat aaagtaattg atttgtttat taatttatta gaagaatctc
    32221 cgcaggaaaa taatgattct gaaaaagtgt agctgagatt gaagttgaat aaagagaaat
    32281 agatattata gaagtagtac ttaagtacac attatataaa aatataatag gtataggatt
    32341 aaaaagttta gtggtttaaa gtttaaccac taaacttttt taatgtctat aatctactat
    32401 tatttaattt actaaaaata aaaaaactca ttatattaag atagaaatag aaaaataact
    32461 agaaaaatac tataatatat cagcttaata cagaaaaaag ttaaaaaatt tgagaaaaat
    32521 aaaaatgaaa acgttttata ttgacataga gaaaaagtag tgataccatt taaatatagt
    32581 ttagtagcta aactatattt aaattataat aactatttta gggggatgtt atgaatttaa
    32641 aacaattaag tgaaaaatac gaagaatatg taattcaaat gagaagagaa tttcataagt
    32701 atccagaatt atcttttaaa gaaataagaa caacccaaag aataagggaa gaattacaaa
    32761 aaataggggt cccttttgta tctattgaac caaatatcgt aatagctact atacaaggca
    32821 aaaatgaagg taagacaatt gcaattagag gagatataga tgcgcttcca atgcaagaag
    32881 attcagatgt tgaatacaaa tcagaatatg atggaataat gcattcttgt ggacatgatg
    32941 ctcatggagc tatgttacta ggacttgcac atatgctaaa tgatataaga aaagatttaa
    33001 aaggtagagt attattagtt tttcaagcag ctgaagaagt tggtggtgga cacaaggaga
    33061 taatacagta ctttaaggaa aatggaggac cggatagatt aatagctact catgtttggt
    33121 ccgatattga agctggtaaa atttctgttg aaccaggacc tcgtatggct ggtgggtctg
    33181 gttggagaat agacataaca ggtagaggag gtccagatca aagcatagat cctataaagc
    33241 cagcttgtga tatagtttta aaagtatctt ctataccagt taatcatata agtgtactag
    33301 aacaatgtgt tgtacatgca tgtgcaatac atggaggaac tacaatagga aatgttattc
    33361 caaacaatgc agaggtaata ggtggatata gatattttac tgaagaatca ggcaaaaaag
    33421 tgtttgacat tataaagcaa atgagtaatg gtgttggaca agtttatgga gtggatgtga
    33481 aggttactca tactggaggc ataggacctg ttatcaatga tgaagaagca gttaagatgg
    33541 ctaaagaaat agtttctaat atagatggat tggagttaga ttcttatgag ccaatatgtg
    33601 catcagattg ttatggtgaa atattaaaag agtattctgg cttttattgt tatctagggg
    33661 taggtaacaa agaaaaagga ataatatatg cacaacacca tcctaagtac aatatagatg
    33721 aagatatatt aaagttaggt tgcgaattta tggctaaata tgcagttgaa tttttaaata
    33781 aaaataataa ctaaaataaa tctaatttta taaatggggg gatgaaaatg caaggtgcat
    33841 taaaggaaaa taagaaaaaa agtggtggag gatttttagg atggattgaa aaagttggga
    33901 ataaaatgcc acatcctcta gcattattta catatattac actgatagta gtagcattat
    33961 ctgcaatagc aagcttttta ggtttttcag caacaagtcc tgctacagga aagttaataa
    34021 aagttatgaa cttaataagt gcaaaaggtt tagtactatt tatgcaagag tttgttaaga
    34081 acttccaaaa cttccctgtg cttggagtag ttttagttat ggcagttgcc acaggagttt
    34141 gtgaaaagac aggatttttt tcaactgcca taaaaatgag tttatcaaaa gttaagggga
    34201 atgcggttgt atttataata gcttttatag gagtatttgc taatcaagct ggtgatgctg
    34261 cttttgtatt agtaccaact cttgcaggag caatatttta tggattaaat agaaatccat
    34321 tagctggtat attctgtgga tttgcatcag ttggtggtgg atttgcaaca gctgtaattc
    34381 ctgggggatg ggacgttacg ctaacgccaa taactgtatc agctgctcaa actattcaac
    34441 ctgcatttga tatgacatta ttagctagtt attatgcact atttgtatct gcgtttatag
    34501 tagctacagt atcaacaata gtaacggtta aatttataga acctaaatta ggtaagtata
    34561 ctggtaaacc agaaggtata gatgataatc aaggttttga ggtcacaaaa gaacaagcaa
    34621 aagcagcaaa gttagcaggt tttacagttt tagcttatgt agcgttatta attgctttgt
    34681 gtataccagc taatagtttt ttaagaagtc cagaaggttc tttaatattt ggagcaccac
    34741 ttatgtcttg cttacagttt tttatagtaa tactattctt cttgccaggg attgtgtatg
    34801 gaatgagggt tggtaaaatc aagactgtaa aagacttaaa tgatatatta tgtcaatcaa
    34861 tggcagcaat ggcaccgttt atcgtacttg caatagttat aggtcaattc ttaacgctat
    34921 tctcagtgtc taatttagca caggtactag ctataaaagg tggagcatta ctcaactcat
    34981 tgccactacc accacaagct ataatactgc tattcttagt actagtagcg tttataaata
    35041 tatttgttat aagcggaagt acaaaatgga tgatatttgg accagtattt gttccaatgt
    35101 taatgcaatt aaacatacat ccagcattta ctcagtttgt atatagatta ggagattctg
    35161 taaccaatca tttatcacca ttaaatgcat tctttataat acttttagca acagtccaaa
    35221 aatacgataa aaatgcaggt atgggaacta tattctcagc tatgatacct tacactatag
    35281 gttactttgt agtacttgca gttttagtag tagtttggat gacaataggt ataccagtag
    35341 gaataggtgg acaagtttgg ttataaaaca tatttaataa ttttgggagg aaaaaaaatg
    35401 gaaacaactg aaataagata caattttgat gaagtaatag atagaagtgg aacaaactgt
    35461 cttaaatatg atcttaagag tcaatttatg ccagaatctc cagaagatgc actaccacta
    35521 tgggtagcag atatggattt tgcatgtgcc cctgagataa ttaatgcaat gcatgaaaga
    35581 atagacagaa aaatatttgg atatagctct caacaaactg atgattatta tgaagctgta
    35641 tgtggatggt ttaagagaag atttgattgg aaaatagaaa aagagaatat attctttagc
    35701 ccaggggttg tgcctgcgtt aggatttttc acacaaatat taacacaaaa gggtgatggt
    35761 atcataatag aaaagcctgt atactatcct tttgaagata aaataactaa caatgaaaga
    35821 aaagtagtta atagtccttt aaagtttgaa gatggtcatt attctatgga ttatgatgga
    35881 tttgaagaat tagctaaaga tgaaaataat aaggtgttta tattatgtag tcctcataat
    35941 ccagttggta gagtatggaa aaaagaagag cttagaaaaa tagtagacat atgtaagaaa
    36001 tatgatttgt ggataatatc tgatgaaata cattgtgata ttattcgtaa aggtgaaaag
    36061 catataccat tagaagtagc atgtcctgat tataaagata gaataataac ttgtacagct
    36121 ccaagtaaga gttttaactt agctggtatg caattatcaa atatagtaat caataataag
    36181 gaattacaag aaaaatggat gcttgaaaca gattctaaat tagctatatt cctacctaat
    36241 ccattcgcaa tagttgctac taaagttgct tataatgatt gtgaagattg gatgaatcaa
    36301 gtaaatgagt atatagatgg taatattaaa tatgtagaag attttgttaa tgagtatatg
    36361 cctaaagtaa agataataaa atctcaagga acttacttag tttggttaga ctttagaggg
    36421 tatggattag atgcaaaaca gcttgaatat gtaatgctta acaaggcgaa ggtattgcta
    36481 gatgaaggat acatattcgg agaagaagga gcaggatttg aaagaataaa tgttgcttca
    36541 cctagaacaa ttatagaaga gtgtatgaat agaataaaat gtgcatttga aggattataa
    36601 tagtaatatc taaataatat atttgtgtat tttaaaaaaa gactatgcta aaaaaatatt
    36661 tcaaagcata gtcttttaaa gttttatgat gagtttaaaa aatttcaaaa cgttactatt
    36721 cacttatatg tttatattag aaataattcc acaatatact cattttctaa agttgcactt
    36781 ttatacacct tgcttcacca atatcatgtg taaccaaaat agtagactta ttctcattct
    36841 taataatact ataaacatca tcacaaacta ataatctagt ctgataatca agagctgaaa
    36901 aaggttcatc taaaagtaga atatctggat ttacagaaag tgttctaata agcgcaactc
    36961 tttgtctcat acctccagat aattcttttg gatacatact tctaaaatcc cataatccat
    37021 aagtctttaa aaggctttca actctatcta aagatttttt atcttttttg ccttgaattt
    37081 ccaatccaat agttatatta tccattatat ttctccattc aagcaaatta tctttttgaa
    37141 acatatatcc aatatttcca gtaatcttta tatcaccact agttggtttt aaaagattag
    37201 taagaatatt taatattgta gatttaccac ttccagaagg accaagcaaa gttaatattt
    37261 ctccttcttt caagttaaaa ttgacatcct ttaatacttc aagctcgcct tccttagtgt
    37321 agaagttcat atttatattt ttaacttcta ctatgttgtc attcattatg ccacccccta
    37381 taacttttag ctcgtatata ttatgttatt aacaatatga cctatttgta ccatttttca
    37441 taaatataat ataaatattt tgtttattta cgtattttct tagtaacttg tacgtataaa
    37501 ttatagataa ataaattata aggtagggga taaatttgag taacataata acgaatttaa
    37561 tagagtcaat atttactaat ccaataacta tattgttttt acttgcaata actgtgtatt
    37621 ctgtttttaa aataatagaa aataacagag tatattcatt tgtaagttca aaattagacg
    37681 aaatcaatag agactataag acaagtgagt tttataaaaa ggtaagggat gactactata
    37741 catatagtaa agaaaatcct tatgcagacg taaatataac ttcatttata gaggaagttg
    37801 tatctgattt aaagcataat aatttgccat tgttagaaaa aattagaagt ataaaaaatt
    37861 catcttcaat aagtatatta cttggggttt taggtacgtt tgttggtctt tctacaatgc
    37921 ttttatgtgt tgatactaaa gacattataa attcacttcc ttcaactatt agtagtatgc
    37981 aaactgcatt tacaactagt atatttggag ttgtattttc tct
//
LOCUS       DAEPIN010000036        37816 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.036, whole genome shotgun sequence.
ACCESSION   DAEPIN010000036 DAEPIN010000000
VERSION     DAEPIN010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 37816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37816
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.036"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            complement(212..1366)
                     /gene="nagA"
                     /locus_tag="KR250_002012"
     CDS             complement(212..1366)
                     /gene="nagA"
                     /locus_tag="KR250_002012"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HBH0067948.1"
                     /translation="MKSFIYADKFFMKHGIKEKGYLSIINGKFGEFQQERPNVTDNVI
                     DFSGKYIAPGLVDTHIHGLLGADVMDNTFEAINTISKGLLKYGVTSFLPTTLTDSVDT
                     LSDSVENISNSFNRVEGAKIQGIYLEGPFFTEKYKGAQNEKYFKDPDINILKLWQKKS
                     KNLIRKIAIAPERKGSIELTRYATKNNISVSLGHSSATFEQVKQVVNSGAKVFVHTYN
                     GMSPLNHREPGMVGAAMVLKDTYAELICDGHHVSPIAVKIMMDAKSSNNIALITDCMR
                     AGAMEDGKYTLGKFNVNVKNSVARLNSGSLAGSVLTMDKAVRNIVDWNIATLEDAIKM
                     ATYIPALSCNIDNVCGSIHQGMEADFIVTDKDFNIYETYISGICKYKVNK"
     gene            complement(1449..2174)
                     /locus_tag="KR250_002013"
     CDS             complement(1449..2174)
                     /locus_tag="KR250_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBH0067949.1"
                     /translation="MIKRNDKRPIYDQLVEILRTKIENEMEPNDRMLSERKICDEYGV
                     SRTTVRLAMAELEHMGYIYKRHGKGTFVAALSQNSQNLMESYSFTDYMRTQGKKPGTK
                     VLSFEVVESTKYFSEKLGITPGEKMIKIMRLRLADDLPMMLERTYLPMKEFIGLTKEK
                     IEQKPLYEIFREDYGEIIKVADEEFSAGIISDKEAKLLEVPGDSPCLKLLRTTYNDDN
                     IVIEFTLSVARSDKFVYRVRHVR"
     gene            2441..3370
                     /gene="pfkB"
                     /locus_tag="KR250_002014"
     CDS             2441..3370
                     /gene="pfkB"
                     /locus_tag="KR250_002014"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HBH0067950.1"
                     /translation="MITVITFNPSIDRMYRVNNINIGEVQRVVSKNATAGGKGLNVSK
                     VCKILKENPLAMGFLGGFNGEFIKNELRKLDIQNKFTKIEQETRNCLNIIEDNKVSTE
                     FLEKGPIVEKSDLERFENDIEEVLKNTKILVASGSYCENMPIDYYEKIGNICRANNIK
                     FILDTSGEALKIALKSKPYLIKPNIDEIRHLLGINIESREEIILSGKKLIEMGAENVC
                     ISLGKDGMVYLNEDNVYYVKVPVVKCINTVGSGDSTVAGFSVGILRGYKIENLLKFAN
                     ACGISNALNMETGFVDLGEIDKYQDLVEVKKLS"
     gene            complement(3499..4416)
                     /locus_tag="KR250_002015"
     CDS             complement(3499..4416)
                     /locus_tag="KR250_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBH0067951.1"
                     /translation="MKNIKMGMIGLGSIAQKAYLPILTKSERFEFVGAFTPNKVKREK
                     VCSDYRIMQFDSIESLAKKCDCIFLHSSTETHYEIIKILLNLGVHVYVDKPLASTVSQ
                     GEELIELSTKKNLNLMVGFNRRFCPMYKEIKNNATEIVSINICKHGLNSLRNVKFDST
                     LIDDYIHVIDTALWLANEDVEISGEDLFLTDNKNLIFVSHKLKGKNFSINTSMHRDSG
                     TKLEQVEILSKGKIQRVKNLNVLEIEEGGNLTLKQSGAWVNILKQKGFEDISNHFIDC
                     IENNIKPAISGEECIKAQRLLEKIINSVK"
     gene            4886..6055
                     /locus_tag="KR250_002016"
     CDS             4886..6055
                     /locus_tag="KR250_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBH0067952.1"
                     /translation="MKMFKLEEQKLKDLGAIITTNEIKQQPELWLETYEIYKSNKEKL
                     SRFIDTISNNHGQFRVIFTGAGTSAYIGNSILPYLKNKNDIRKYIFEAIPTTDIVSNP
                     YDYLKKDIPTLLISFARSGNSPESLAALNLGNKIVDNFYHLAITCNPEGELAKMTKND
                     ENNYLLLMPSKSNDEGFAMTGSFSCMMLSAMLIFDSLEDDVEKSYINAIVEMGRNVID
                     RKDEIHELINKDFDRVVYLGSGGLGGLTQEAQLKLLELTAGKISTVYDSPMGFRHGPK
                     SFIDENTLVFEFVSNCLYTRKYDLDVLEEIKRDKIAKFTCAVSVENENNFSGTKFEFK
                     EKYNKLPDVYLAMPYILFAQTIALFVSVKVGNKPDTPSATGTVNRVVKGVTIYEY"
     gene            6104..6955
                     /gene="gatY"
                     /locus_tag="KR250_002017"
     CDS             6104..6955
                     /gene="gatY"
                     /locus_tag="KR250_002017"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HBH0067953.1"
                     /translation="MALVTTTSMLKKAQDEGYAVGAFNVENMEMVIAVIEAAEELNSP
                     VILQTTPSTVKYAGLDYYLANAKVAAERVKIPVAMHLDHGSSFDLAMKALRSGYTSIM
                     IDGSHESFENNISISKSVVKACSPSLIPVEAELGKVGGKEDDLDGGNSNDYTNPKEAK
                     EFVDRTGISSLAIAIGTAHGLYKGEPKIDLDRLSEIKNVVSIPLVLHGGSGIHDDIIK
                     EAIKRGIAKVNYATELRIAYSKGVKKVLDEDSEVIDPKKYGLAGLQSVKDFVKEKIKV
                     CGSVNRV"
     gene            7281..9182
                     /locus_tag="KR250_002018"
     CDS             7281..9182
                     /locus_tag="KR250_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBH0067954.1"
                     /translation="MRERIILETLKNSSGYLNIDYFADKLGVSTRTIRNEIKKIETIK
                     ECNGFKLEYKSKLGYILNIKDQDKFEHYLRNLPSDLVENPEQRLESIIVELLVNEGYK
                     TIEQLSKKFLVSSSQIKNDLKKIDEKIKDTELKLERKAHYGIKIEGSIKEIQSILVDS
                     YFRGNRNITEYRNKFIDNLKLANIRSTIKNVLNEHDLEANLTELEEILAQIIILYIRV
                     SMRVAGNASDLKLYTEDLIIDKLLDKIFRDKKYNLNYDEKYYLKELIKLKTKDKKATI
                     KNIDKNKLQDIMFEFFREIDKKYNSNFLEDKEFFNLFYLHIACLIERIKKNHKIINPF
                     SVKISQQYPTVFNLSIQFSKIIENEYHIKISQDEIGFIATHIAVPFEKREEANFNKKY
                     KIAIICSSGGGSAFLINLRLKEIFPNAEIRNFSLLEEKAVIEFAPDLIFSITNLLFEI
                     NAPVILINEILDELDYLDIKESVRFADHIGNISSPKQYILGLFDKNHFRCIKDKAKYK
                     DILDSMSKCIVDEGACSPTYPKDVWERESYLSTIYTNGVAIPHPIEMTGNKNIISVAL
                     IQTDIIYENRVPKIIFMISLIKGNLELHKQISKYLSKIMANKDMVDMLNKSQSYEEFM
                     YKLKIYLGG"
     gene            9185..10228
                     /gene="ypdE"
                     /locus_tag="KR250_002019"
     CDS             9185..10228
                     /gene="ypdE"
                     /locus_tag="KR250_002019"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBH0067955.1"
                     /translation="MNIEFFKRLSEADGIASNEEEVRHVLLEELKAYSDKIICDGLGS
                     IIFSKIKDESAPNVMICAHMDEVGFMVRSIDKLGMIHLITIGGVKPLAQFVQKVRITT
                     KEGKKIPAVINATYNNGKAENIYADIGAYTEEDVYNLGINVGDMVTYTTSFEEFTLPD
                     RLVGKAFDDRIGCFVMGEVLKELRKENLNCNIHFAATSSEEVGIRGAKTSTQLINPDI
                     VFVIDVACAKNEFVRDYTNQKQIDKGVMLMHRDRTLVPSRKMIDYVMEIADKNNIPLQ
                     HDMFESGGTDGGEAHLVNEGKPCVVTCVPVRYGHCAFSIVSNKDLENIIRLYTQLILN
                     FDEEQYKHIRNFI"
     gene            10243..10548
                     /locus_tag="KR250_002020"
     CDS             10243..10548
                     /locus_tag="KR250_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBH0067956.1"
                     /translation="MSTEVQIMELISTAGESKSKAFEALKKVKSKDFKGAKLLIEEAR
                     KIDIEAHRIQTELITQDLNEDVESPAMSLLMVHAQDHYMTSQLAKDLIEALIYCLDK"
     gene            10645..10980
                     /locus_tag="KR250_002021"
     CDS             10645..10980
                     /locus_tag="KR250_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBH0067957.1"
                     /translation="MKILLCCAGGFSTNMLMQNMKKVVKESAKLNEKDFEFTAIPADS
                     IESEVDDWDIVLIGPQIAHKIDFLKGILEPKNKPFAVIDKDVYGKMDGATVMKLALIT
                     YKKHQLAQG"
     gene            11012..12334
                     /locus_tag="KR250_002022"
     CDS             11012..12334
                     /locus_tag="KR250_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBH0067958.1"
                     /translation="MFSKFEAFMNRVFMPLAHKVDNQRHLGSIKAGMVAMTPFTILGS
                     IFAILPALPNMLGENNPISKFILNNSAILDLPVSLSIGLIGLYACMCIAFNLGNYYKL
                     YIPGCVALSTFSFLFLVASFTPDGALEIGNLGAKGLFTGMIAGLGSVELYNFCNKKNI
                     TIKMPENVPDFVSRSFELIPTTIIVCGTFIVARFISLKVFGELPPQILTQFLSPLVGS
                     MDNPWSVLALNFAICTIFFFGIHSSVFSPITRPIMITFIAENIAAMQAGETIPHFFTA
                     GASSAFFGFTGCGISIGCVIACMLSKNASYKKIGRVSLFPALFGINEPILFGAPIILN
                     PIMFIPHVFGGAIIGTLPMFFMHWGWLAKPVFDPPYVGVFLEGFLTNGDWHSILANAL
                     QLVLAVLLYWPFFKIMERQQNNKKQKEETKNIFSKEEEDLLDDLGLDF"
     gene            12344..13414
                     /locus_tag="KR250_002023"
     CDS             12344..13414
                     /locus_tag="KR250_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBH0067959.1"
                     /translation="MNNTMERLVSLIYKRGVDALLIKAKNTKRYIGALSGSGVYVLVT
                     KDKNYQILDGRYIDEADKKTTGFIKRIVSQGSYIPTIIELLKELNIKKLAIENQAMSI
                     QEYLSLKNEGFEINLITNELWKLRAIKSKEEIELIKKACEITDEVFDEVISEIKKDMT
                     ELEVSALIQYHALKKGASGMSFETIVVSGERGAMPHGRPTNKKLKVNEAITIDFGVVY
                     QGYQSDMTRTISIGKPPKIIKEIYDVVLEAQLSAIESIKEGTRASDVDKVARKIIDKH
                     GFGEYFNHGLGHGIGLGDGEVPTLNPNSEDILVEGMVMSCEPGIYIPNVGGVRIEDDI
                     VIIDGKGVPLNKTSKEFIILGE"
     gene            13419..14606
                     /locus_tag="KR250_002024"
     CDS             13419..14606
                     /locus_tag="KR250_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBH0067960.1"
                     /translation="MKYNFNKVLNRKEGNCRKWSNHVIKEKFGLNEDAIPMDLADIDF
                     ECAPAIKESILKRASVGDYSYTFIGDDFYDAIINWNKRRFDVYIEKDWIKLTFGTVST
                     LHYIVQAYTKEGEGVLINTPAYDPFAEAVVNNNRKLCCSSLKLDNNRYYLDLEDMENQ
                     MKYENIRLLIFCSPQNPSGRIWTKEELYQVSELCLKYNVILVSDEIHRDIIFKDYRFV
                     SLWNAHPEIYKNSIICVSPNKGFNLGGLKTSYVLIRDVKIRETLLERLKSNSITSPNV
                     FAIPAIVAAYNESEEWLDAMTSYVEENFEIVYDFFETNIPKAKVMKSDSSFLAWIDVR
                     EVFRNEEESKEFFRHANLTMVVGSYFVKDGDGFIRINVGCPRETLNEALNRIKKTYIS
                     MYC"
     gene            complement(14803..15675)
                     /locus_tag="KR250_002025"
     CDS             complement(14803..15675)
                     /locus_tag="KR250_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HBH0067961.1"
                     /translation="MNILQNENLIIESSSFGAELTKIFSKKYDKNILWDGNKKYWGRQ
                     SPILFPIVGKLFDNETIIEENLYSMTQHGFARDMDFEIVDKGDTFVTYKLTDNESTLK
                     KYPYSFELIINYTLNDNSIEVEWIVKNTDSKDIFFSIGGHPAFNLPFYEQNDFSKHYL
                     EFKSKNDVEKINLNGSFTDDIIPIGKLKNLQLNPEVFKNDALIYTNIDEVSICNNDGS
                     KYVTISMEDFPLVGIWTPYYSETNSTAPFLCIEPWYGLADSINSNKIYKDKKFINKLS
                     KGKVFTASYNINIH"
     gene            complement(16069..16260)
                     /locus_tag="KR250_002026"
     CDS             complement(16069..16260)
                     /locus_tag="KR250_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0067962.1"
                     /translation="MDDFSKLKSLYEDGYRCIYHDCVDNNYTIYLKNFYTEGSETIEL
                     SSESDFSQFKDYIDGLRMS"
     gene            16480..17526
                     /gene="cobT"
                     /locus_tag="KR250_002027"
     CDS             16480..17526
                     /gene="cobT"
                     /locus_tag="KR250_002027"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HBH0067963.1"
                     /translation="MSLLESISKNIYSLDNSSIEKTKQRLDRLIHPTGSLGKIEDICM
                     QLAGIFGNENFDTSKKVIIAFAGDHGVYEEGVAPDPQNITKLQFPNFSKGLCGVGVIS
                     KFVGADVVAVDVGINCDEKLDGVLDYKIRKGTSNMAKGPAMSKQEAIRCLEIGIEIAE
                     QCIEKDYKVIGIGEMGIANTTPSTAIISVISGCDPLEVTGIGAGLKKERLKHKAEVIR
                     KAIEINNPNPTDGVDILSKVGGFEIGSMAGVILGCSANRIPVVIDGFISYAAALIAYK
                     INPKTREYMIASHLSAESGTKRALDILKLDPLLNMDMRLGEGSGAALAFDIIEASNYT
                     YKNMATFDEIDMGR"
     gene            17558..18118
                     /gene="cobU"
                     /locus_tag="KR250_002028"
     CDS             17558..18118
                     /gene="cobU"
                     /locus_tag="KR250_002028"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HBH0067964.1"
                     /translation="MSKIILVTGGARSGKSNFAEKLCFDRNNNTAYIATSIPFDDEMK
                     DRVKKHQENRPRNWNTYEIYEDIYSIIKEIYKEHETVILDCVTLLVNNLMFEHNLNIE
                     ESSQEEINKLEQYIKEQVVKLIEEIEKTNLYFVFVTNELGMGIVPGNKLSRIYADIVG
                     RINQYIASKSNEVYFVVSGIAMKIKE"
     gene            18128..18901
                     /gene="cobS"
                     /locus_tag="KR250_002029"
     CDS             18128..18901
                     /gene="cobS"
                     /locus_tag="KR250_002029"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HBH0067965.1"
                     /translation="MKRFILILQFLTRIPIKLNVGFDDEFYKSIVYFPLVGFVIGILS
                     YLIGWISMLLFEPFIASIIITLAGVLITGGLHIDGLGDTFDAIYSYRDKEKMLEIMKD
                     SRLGTNSLLAIMFVLLLKVGFVYDIISNNSLWVIIFMPMIARLGVMLLTYKTVTPREK
                     GMGNLFIGKLTTSMLITAIIYTLLIVALITKFIFLLPNIVLIKVLGSIIVVFVFIILF
                     KKHIYKKIDGVTGDILGCGIELSELVYLIYIYLLIFMFF"
     gene            18921..19535
                     /locus_tag="KR250_002030"
     CDS             18921..19535
                     /locus_tag="KR250_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBH0067966.1"
                     /translation="MIRLILIRHALTNDNKKGRLSGHINSCISEEGKLQINKITRYLS
                     NENIDKVYTTPSTRTKDTVEKISKLKLLEIEEKEALREISFGDFEGRTFEEIKIKYPN
                     EFEKMIKEGNNYRYPNGESLIDSYKRVAKEIDNIILENDSNLDTKTILICSHAGTIRN
                     IITHLISGSYKYHWNFKIDNASITVLEIDGGFAVIDKMNFTDFI"
     regulatory      19602..19781
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            19911..21410
                     /locus_tag="KR250_002031"
     CDS             19911..21410
                     /locus_tag="KR250_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HBH0067967.1"
                     /translation="MLQGTASNVGKSVLVAGLCRIFKQDGYTVAPFKSQNMALNSFIT
                     KEGLEMGRAQVFQAEAAKIEPIADMNPVLLKPSGNNKSQVIVRGKVVGEMPSSKYHDY
                     KLELVDVLKETFNSLNSRYDIVVMEGAGSNAEINLKDRDISNMGMAEIADAPVIIVGD
                     IDRGGVFASLAGTMLLLNEDEKRRVKGVIINKFRGKKELLQDGVKMLEDIIKVPVIGV
                     VPYTDIKIEDEDSVTTRFKKKMNKSDIHIEIIRTPHMSNFTDFNIFETQEDVSIRYVD
                     YGESLGNPDILIIPGTKSTIDDLTYIRKSGLESQIKNLHSQGKLIFGICGGYQMLGKK
                     LKDPYHVESEIEEVDGIGLLDTETTFEKEKTTTQVEATIVECISEYMNNLKGKKVKGY
                     EIHMGITKRGNDVRSLDLITKKLDKEVNYTEGSVNKEGNVIGTYLHGIFDEISFTRAI
                     LNNIRKKKGLGELESKVNSFNEFKDKEYDKLADFLREHLDIEKIYEIMK"
     gene            21449..22825
                     /locus_tag="KR250_002032"
     CDS             21449..22825
                     /locus_tag="KR250_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HBH0067968.1"
                     /translation="MKKILIAGTNSGVGKTTISLGIMQALVKRNMKVQPYKVGPDYID
                     PSYHTFITGRHSRNLDSYMLDDEKIKYIVNKSSKDADISVIEGVMGLYDGFGIDLDNC
                     TSSHTSKVLKSPVILVINGKSMAASSAAMVLGYKELDKDVNIKGVIVNNVKTNSHYQI
                     IKSSIEKYCNVEVLGYFPPNNKFSLESRHLGLVPSVEMKSLREKFYTLADEIEHYIDI
                     DRIIEISESDEFESDFDFQTLIENNKIKEHVNNKSIAIAYDKAFNFYYRENIELLEEL
                     GLEINYFSPLEDVSVPNADYIYIGGGFPEIFGKELEANESMRTSILEAHNNNIPIYAE
                     CGGLMYLGEKLLNQEEQEFNMVGIFSGISKMTSSLKRFGYCIGVAKENTLLSKKGESI
                     KGHEFHHSIFESDEKCVYSMRKERDGNIVEEWDGGYSKGNTLATYLHTHFYNNLNCIE
                     NFLKKGNE"
     gene            22826..23797
                     /locus_tag="KR250_002033"
     CDS             22826..23797
                     /locus_tag="KR250_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HBH0067969.1"
                     /translation="MSILSIYIGYVTDLIVGDPYSFPHPVRFIGKLINFTQGIIRKVS
                     KNDRQLKLGGFILWFITVGITYLTTYAIVKLFSFNILLSIVVNSFIIYTTLATKCLKD
                     EALKIYNVLKTEDIEKSRIQLSYIVGRDTTNLSEPEIIRATVETVAENTVDGIIAPMF
                     YAFIGGAPLAMAYKAINTLDSTVGYKNKKYKDIGFASAKIDDIANYIPARISVILMTI
                     GSFFLNYNYRNCFKISIRDRKNHKSPNCAFSEGAVSGALGIQLGGTNVYFGEKVYKPT
                     IGDKLREIDKEDIIKTNKIMYASSFVSMLVFTLIYVLCRMILINLII"
     gene            23829..24899
                     /locus_tag="KR250_002034"
     CDS             23829..24899
                     /locus_tag="KR250_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBH0067970.1"
                     /translation="MKDLGHGANVDEMARLYGKNPKEIIDFSSNINPNVLPNLERYIL
                     KGLEECRNYPDINYTNLRENISKYIDINPDFIIPGNGATEVIYLLMKSIKKKLAIINP
                     TFSEYRRSAELNNLDIIDLELDLENNFKLNIDIIKENIKRFDSLFICNPNNPSGNVQD
                     LKELVHLLDKHNKVLIIDETFMEFVEDESKYSLVKYIESNKNIFIIKAVTKFFGMPGL
                     RLGYGLTSNTEIMNKIYEHKEPWTINSFADILSNFIFEDKEYIKNSKEYYIEEREYML
                     QELRNIRNIKVYDTDANFILIKIYKKTTKELKKDLFKQGNILVRDASNFIGLDDSFIR
                     VAIKSHEDNKILIENMKNLLGD"
     gene            24901..25788
                     /locus_tag="KR250_002035"
     CDS             24901..25788
                     /locus_tag="KR250_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HBH0067971.1"
                     /translation="MKSYGICPASCGEFVQGIIDDEEYLCSYAIDMYSKVYIEEKLVD
                     INLGRYKSRLAIEKVFEKFNLPKKYTKNISLNINSKIPVGKGMASSTADIGATIKATL
                     SLIDKDLSSEEISKLAAEIEPTDSIFIDKNSIFNPLNGTVIKYLGNLTNAKVVILEPN
                     KVLDTMKIRLRQDYNKLKVENKEVIKKSFALLEEGLKKNNLSLVGEACTLSSLANENI
                     EKKEYLNEIIKISKKYGAYGVNIAHSGTVVGILIDKFMNDKKMIDALCESNINSVYNK
                     IYTQNIINGGIKGEIEWNT"
     gene            25776..26408
                     /locus_tag="KR250_002036"
     CDS             25776..26408
                     /locus_tag="KR250_002036"
                     /EC_number="5.4.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003724727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-8 methylmutase"
                     /protein_id="HBH0067972.1"
                     /translation="MEYIKNPMKIEEKSFELIQEIIDEIRPDYKFKNNIEEKIIKRAI
                     HTTADFDYLDILKISEEAVDSIIDALKNNASIYTDTNMALSGINKRKLESLGCKYKCL
                     VADDETVKLAKEKGITRSMAAVEIAAKEEGRKIFVLGNAPTALYKVMEMKSEGRLDLD
                     AVIGVPVGFVGAQESKDEVEKTDIPYIISKGRKGGSNLAAAIVNAILYTM"
     gene            26417..27643
                     /locus_tag="KR250_002037"
     CDS             26417..27643
                     /locus_tag="KR250_002037"
                     /EC_number="2.1.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-5B (C(1))-methyltransferase"
                     /protein_id="HBH0067973.1"
                     /translation="MEEYVYIDGKKYRRGYTTGSCATGASKAAVYMLITKNRINTINI
                     DTPKGIPLLLKVDNINISDTFVECSIKKDGGDDIDATHTMDIYARAEIVAKNDKNKGY
                     LTLKDIDSLSTNSECKSELYKFIRVYGGTGIGVVTKKGLSVDVGKPAINPTPLKMINH
                     EIRKLIGDNFESILGNDKVLKITIFAPQGETVAKKTFNPRLGIVGGISIIGTTGIVEP
                     MSDDGWKKSLSIELQMKKEQGLDKIILVPGNHGEQFIREKLNLDIKYVVRVSNFIGYM
                     IKEAQRIGYKKILMAGHIGKFIKVSAGIFNTHSKVADARSEILVANLALMGARYEFLN
                     KINQCVTTEEAVELINNSEYREVYNILSNKCRERVKQYLNEDSDDIDVEVIIFSMDKS
                     LLGKSDNTDDLVEVFI"
     gene            27640..28248
                     /locus_tag="KR250_002038"
     CDS             27640..28248
                     /locus_tag="KR250_002038"
                     /EC_number="2.1.1.289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-7 (C(5))-methyltransferase"
                     /protein_id="HBH0067974.1"
                     /translation="MINIIGLGPGNLDYITKKGENLISTSDVLIGGKRNLESIKNFEG
                     EKIVLDSNLREIIEYINNNKEKQISIIASGDPLIYGIGRYLSKNIDNKMLNMVSGISS
                     LQYIFSKIYVDMNDVYITSSHGKVPDFDYILSHKKVCMVTDSKIGPKQISREIIDRNL
                     NKIIVVGENLSYDNEKITIAKPEEIIRMDNFDMNVVVILDEE"
     gene            28238..28813
                     /locus_tag="KR250_002039"
     CDS             28238..28813
                     /locus_tag="KR250_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating cobalt-precorrin-6B
                     (C(15))-methyltransferase"
                     /protein_id="HBH0067975.1"
                     /translation="MRNSEFITGKVPITKEEVRAISISKLNLVNAKNLIDIGAGTGSV
                     SVEAAYNYPNLKVISIEKNDDAIELIEKNKKKFNLKNIEVIKGYAPINLGLNVKVESI
                     FLGGTGNNLEEIIEWSKKNLVTGGRLVANFIIIDTFNQTLKLLKKHGFKEIDVCVLNV
                     SKLEKLGKGEYFKPLNPIYIISCEKGENDDE"
     gene            28806..29558
                     /locus_tag="KR250_002040"
     CDS             28806..29558
                     /locus_tag="KR250_002040"
                     /EC_number="2.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-4 methyltransferase"
                     /protein_id="HBH0067976.1"
                     /translation="MNKVHFVGAGPGDKELITLKGYKLLSNADVVIYAGSLVNPELLE
                     YCKEDCQIHNSAHMDLQEIIDVMREGIENNKSVVRLQTGDFSIYGSIREQVEDLNKLN
                     IDYDCTPGVSSFLGAASSLGVEYTVPEISQSVIITRMEGRTPVPEKESIQSYAKHQTS
                     MVIFLSVQEIEKVVSKLLEGGYPKDTPIAVIYKATWADEKIVKGTLSDIAVKVKENNI
                     NKTALIMVGRFLGEEYNNSKLYDKDFKHEYRG"
     gene            29542..30663
                     /gene="cbiG"
                     /locus_tag="KR250_002041"
     CDS             29542..30663
                     /gene="cbiG"
                     /locus_tag="KR250_002041"
                     /EC_number="3.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin 5A hydrolase"
                     /protein_id="HBH0067977.1"
                     /translation="MNIEGNIAIICITENGKKLALKINSLVNNCVIYHVKNKKSNLVV
                     EETCVHTVQKKLSDFVGEIFSKFDYIVFIMATGIVVRSIAPYIISKFSDPAILVMDEK
                     GKNVISLLSGHIGGANSLTQYISSLIDANPVITTATDINQKASLDMIAKKLNAHIDNF
                     RESVKEVNSMLVNNKCVGLYIDEGYQVDTRGFKLIESKDELRDSFSNTYIEKLVVVSN
                     KINCIEDIENLNLDNKFEHCYSTKKIIKVIPRDIVIGIGCRRNTDSELMYNSLEEFLN
                     KQNIDIRAINTVGSIELKKDEKAIIELSKILKVPFKTFSVDEISKVDNLFDKSDFVKK
                     NVGVYSVAEPVAYLLSKGNLLVEKHKYKGITFSVGRIKI"
     gene            30660..31385
                     /gene="cobJ"
                     /locus_tag="KR250_002042"
     CDS             30660..31385
                     /gene="cobJ"
                     /locus_tag="KR250_002042"
                     /EC_number="2.1.1.131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011701973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-3B C(17)-methyltransferase"
                     /protein_id="HBH0067978.1"
                     /translation="MIYVVGIGPGSKDTMTLEAIKAIEDSEAIVGYKTYIKLIEEFIQ
                     DKEIIQNGMRQEVDRCKQAVEEAKKGKKVAVVSSGDAGIYGMAGLILELISKEDEDIK
                     VKVIPGVTASIGAAAILGAPIMHDFCHISLSDLMTPWEVIEKRLRLAAEADFVICLYN
                     PRSKGRSEHLSKAFKIMGEFKGGDTPVGVVKDVGREKEEKFVCTFENMDFEKVDMTTM
                     VIIGNKSTYINGELMITPRGYTV"
     gene            31382..32134
                     /gene="cobK"
                     /locus_tag="KR250_002043"
     CDS             31382..32134
                     /gene="cobK"
                     /locus_tag="KR250_002043"
                     /EC_number="1.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6A reductase"
                     /protein_id="HBH0067979.1"
                     /translation="MILILGGTSDSLSICDKINELKNQPYILSVTTDYGRELAQQHAE
                     NVILGKLGKEDMLKFIKDNNIVKIIDATHPYAVEVSKTAITCAKLLNIDYIRFERKSL
                     IEEIDYDNKFIVDTIEEACEIANKIGTNIFIGTGSKNLNRFIEEISDKNLVVRVLPTS
                     EVILSCEKLGLSADNIVAMKGPFSQYINEETYKHYNIDLVITKESGIAGGFLEKVNAC
                     KTFNIPVIIIKRESINYENVFNDINKIKDILV"
     gene            32153..33631
                     /locus_tag="KR250_002044"
     CDS             32153..33631
                     /locus_tag="KR250_002044"
                     /EC_number="2.1.1.151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-factor II C(20)-methyltransferase"
                     /protein_id="HBH0067980.1"
                     /translation="MKKALLVVSFGTSYHETREKTIDICEDKIKNSLKSHDFFRAYTS
                     NMIINKIKKRDGIEIDNPIQALDKIYEQGYDEVIIQTLHIICGEEFNKLKEQVDGYSS
                     KFKKIVLGRPLLTHIEDYQEAVEAIKHQIPHMESNEAVVFMGHGTLHESHSAYPALEY
                     MLRDNGINAYVGTVEGYPEIEHVIRRLKEGNIKTVNLMPFMLVAGDHAINDMAGDEED
                     SWKTILEENGFNVRVHLKGLGENSYIQDKFVRHAVKCAENAKFYGIGAGPGDGSLLTI
                     KAVNTLKKLDILYTPESKKGGDSLALSIVSEYLPESVEIKSRHFPMSFDGNEKSLAWD
                     NVAQEIISDVNDGKNVGFVTLGDPMIYSTYVYIMKRILGEVEVETIPGISSFSNIASN
                     QNFPLVMDKEALVVIPCTMEDDKIEYALQNYNSIVLMKVYKNFKEIIKKLEKYDLIEH
                     AILVSNSSQESENVFTDLKEAHLEDKISYFSTILVNKDNKIK"
     gene            33661..34566
                     /gene="hemC"
                     /locus_tag="KR250_002045"
     CDS             33661..34566
                     /gene="hemC"
                     /locus_tag="KR250_002045"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HBH0067981.1"
                     /translation="MNIVVGTRGSNLALIQTEWVINELKKKYPEISFEIKIIKTKGDL
                     IQNVSLDKIGDKGLFVKEIEQQLLDGKIDIAVHSMKDMPSYLADGLKFAHTPKREDPR
                     DVLILREGYKNLDDLPHGAVIGTGSKRRKFQLLKQRPDLNIVQVRGNVETRIRKIKDE
                     NMHGIVLAASGIIRANLQDKISSYLPVDVVIPAPAQGALAIEIRSNDSAIEGIVNSLK
                     DENTEIQILAERGFLDGVNGSCHIPMAAYCEIIQDKIHLTGLYGDSEGKKVVIKSIDG
                     DISSPRELGLKLAKLVLKEYENYEG"
     gene            34556..36055
                     /gene="cobA"
                     /locus_tag="KR250_002046"
     CDS             34556..36055
                     /gene="cobA"
                     /locus_tag="KR250_002046"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HBH0067982.1"
                     /translation="MKGKVYLVGAGPGDYKLMTLKGLECIRKSDVIVYDRLANSSYLR
                     EAKPDCELIYVGKASSNHILPQEDINRIIAEKAKEGKIVTRLKGGDPYVFGRGGEEAE
                     TLVDEGIEFEVVPGITSAIGGLCYAGIPITHRDHASSFHVITGHLKGDDSGELNWNAL
                     ANNKGTLVFLMGISNLEKISENLMKEGKDKDTPVALISWATRYNQRVVTSTLENVYET
                     AIKEEVKPPTLIVVGSVVGLREKLNFFESKPLFGKSIAVTRSRNQNSVLVEKIMDLGG
                     NPIEIPTIKVEKIQNNINLENEIKNINKYNYLILTSKNAVEIFFEKIYEMNFDLRILS
                     NLKVCAIGSATSNELKKRGIIADIVPKKFVAESLYEELAPILNDDDNILIPRAENARD
                     YLVDKLKAICEVTEVHIYRTVIDEEKKGEILDILNSNDIDYITFTSSSTVNNLVEIIG
                     KENIKILEKIKTLSIGPITTETINSLGIKLYKESATSTIDSLVDLIIKN"
     gene            36072..37037
                     /gene="hemB"
                     /locus_tag="KR250_002047"
     CDS             36072..37037
                     /gene="hemB"
                     /locus_tag="KR250_002047"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HBH0067983.1"
                     /translation="MLRRPRRLRASKAIRNLVRETKVNVEDLIYPLFVVEGENIKEEI
                     KSLPDVYHLSIDMLEDEIKEISELGIEHIMLFGVPDEKDECASQAFNDNGIVQKAIRK
                     VKEINSDINVITDVCMCQYTSHGHCGILTDTGYVDNDKSLEYLAKISVSHAKAGADMV
                     APSDMMDGRIQAIREALDENGLENVGIMAYSVKYASNYYGPFREAAHSAPSFGDRKTY
                     QMDPANSNEAIIETELDILEGADILMVKPALSYLDIIRRVKDNFDLPLAAYNVSGEYS
                     MLKFAVKEGLLNEDVILESITSIKRAGADIIITYFAKDIAKLLKK"
     gene            37190..37768
                     /locus_tag="KR250_002048"
     CDS             37190..37768
                     /locus_tag="KR250_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HBH0067984.1"
                     /translation="MLYPINLKLDELDVVIIGGGEVAYRKCKNFLEFNKNVTIVSKQI
                     LNKFYDLKGNIKIIKDDYKEIYIKDASVIIAATNNRELNMEIGLYCKAKNKLVNVVDN
                     IEISNFTVPSYIKRGDLLISVSTGGKSPSLSSKIKKEIEERYTEDYEEYLNVLGDIRK
                     KVIKKYEDKSKRKDVLNMLVTLDLEELKKFDI"
BASE COUNT        15071 a         3984 c         6727 g        12034 t
ORIGIN      
        1 ttacaaataa taatagaaaa gttactttaa atgtacatat tatcgaaaat attatcacaa
       61 aaatacttat aatatatatt tgtttaaaat aacctctatc gcaatagttt attaacgata
      121 tggaaataaa agtgttaaat cagattttgg agaaataact ttaaatattt ccagactctt
      181 tttagacagc ctccataacc ttatattgaa cctatttatt aactttgtac ttacatatac
      241 cacttatata agtttcgtat atattaaagt ccttatctgt tactataaaa tctgcctcca
      301 taccttgatg tatggagcca caaacattat caatattaca acttaaagca gggatatatg
      361 tagccatttt tattgcatct tctaaagtgg ctatgttcca atcaactata tttctcacag
      421 ccttgtccat agttaataca cttccagcta aactcccact atttaatctt gcaacactat
      481 tttttacatt tacattaaac ttaccaagag tatattttcc atcctccata gcaccagctc
      541 tcatacaatc tgtaattaaa gctatgttat tactagactt agcatccatc attattttaa
      601 cagctattgg gcttacatga tgaccatcac aaattaattc agcataagta tcttttagta
      661 ccatagcagc cccaaccatt ccaggttctc tatgatttaa aggactcatc ccattatatg
      721 tatgtacaaa cactttagca cctgaattaa cgacttgttt aacttgctca aatgtagctg
      781 aactatgacc aagagaaact gatatattat ttttagttgc atatcttgtt aattctattg
      841 aacctttacg ttctggagca attgctattt ttcttattaa atttttcgat tttttttgcc
      901 ataattttaa tatattgatg tcagggtctt taaaatattt ttcattttga gcgcccttat
      961 atttttctgt aaaaaatgga ccttctaaat aaatcccttg tatttttgca ccctcaactc
     1021 gattgaagct attgcttata ttttctacag aatcacttag tgtatctaca gaatcagtta
     1081 aagttgtagg taggaaagat gttactccat attttaataa gccttttgaa attgtgttta
     1141 ttgcttcaaa tgtattgtcc atgacatctg cacctaaaag accgtgaatg tgagtatcaa
     1201 ctaagccagg agcaatatat ttacctgaaa aatctataac attatcagta acattaggtc
     1261 tttcttgttg aaattcaccg aatttgccat taattatact aaggtaacct ttttctttga
     1321 ttccatgttt cataaaaaat ttatctgcat aaataaaact tttcaaaata acctccttat
     1381 aattatatta tcattgtggc attgacgctt ataatatcat tctgttttct tgaaagttat
     1441 ttaacttact atcttacatg tcttactcta tatacaaatt tatcacttct agcgacactc
     1501 aaagtaaatt caataactat attgtcatca ttatatgtgg tacgaagtaa tttcagacaa
     1561 ggtgaatctc ctggtacttc caataactta gcttccttat ccgagattat tccagctgaa
     1621 aattcttcat cagcaacttt aataatctcg ccataatctt ctctaaatat ttcatataaa
     1681 ggtttttgtt cgattttttc ttttgtcaat cctataaatt ctttcatagg aagatatgtt
     1741 ctttcaagca tcataggaag gtcatcagca agtcttaaac gcattatttt tatcattttt
     1801 tcaccaggag ttatacctaa tttttcagag aaatactttg tactttctac tacctcaaat
     1861 gataatacct ttgtacctgg ctttttccct tgtgttctca tataatctgt aaaactataa
     1921 ctttccatta agttttgaga attttgactt agtgcagcaa caaaagttcc ttttccatgt
     1981 cttttgtata tatatcccat atgctctaac tcagccatag ctagtctaac tgtggttcta
     2041 cttactccat attcatcaca aatttttctt tcggaaagca ttctatcgtt tggttccatc
     2101 tcattttcaa tcttagttct taatatctca acaagctggt catatattgg tcttttatca
     2161 tttcttttta tcataaaaat atagtccttt ctaagtggtt attaccacaa tttgattata
     2221 gaatatagaa ttatttatgt caatgaataa agtttaatca aaattcaata agtaaggcta
     2281 ttaatttatt ataaaattat ggtacattga caaaacaagt aaaataattt atattcaaat
     2341 tggtaataac cactttggat atattatatg aggtattttt tattttacag attaatttat
     2401 tgtttagaaa agtaaaaatt aataatgagg agaatctagt atgattacag taataacatt
     2461 caacccatct atagatagaa tgtatagagt gaataatata aacataggtg aggtgcaaag
     2521 ggtagtaagt aagaatgcaa ctgctggagg taaaggatta aacgtatcta aggtatgtaa
     2581 aattttaaaa gaaaatcctt tagctatggg atttctaggc ggatttaatg gagaatttat
     2641 aaaaaatgaa cttagaaaac tagatataca aaataaattt acaaaaatag agcaggaaac
     2701 tagaaattgc ttaaacataa ttgaggacaa taaagttagt acagaatttt tagaaaaagg
     2761 accaatagta gaaaaaagcg atttagagag atttgaaaat gatatagagg aagtactaaa
     2821 aaatactaaa atactggtag cttctggtag ttattgtgag aatatgccga tagactatta
     2881 tgaaaagata ggaaatatat gtagagcaaa taatataaag tttatattag atacatcagg
     2941 agaagcatta aaaatagctt taaaatcaaa accatattta ataaagccaa atatagatga
     3001 aataagacat ttattaggaa taaatataga atcaagagag gaaattatct tgtcaggaaa
     3061 aaaacttatt gaaatgggtg cagagaatgt atgtatatct ttaggaaaag atggtatggt
     3121 gtatttaaac gaagataacg tatattatgt aaaagtacca gttgtaaagt gtataaatac
     3181 tgtaggtagt ggagatagca cagtggctgg atttagtgta ggtattttaa ggggttataa
     3241 aatagaaaat ttacttaaat ttgcaaatgc ttgtggtata agtaatgctt taaatatgga
     3301 aactggattt gtagatttgg gagaaattga taaatatcaa gatttagttg aagtaaaaaa
     3361 gttaagttaa atacatttaa tgggaatata ataaatttaa tgttgttgta atagatttta
     3421 attttaatat aaaagatata gtcttatata aaaataggtt agaaaaattc atttttaacc
     3481 tatttttata ataaatatct attttactga atttataatt ttctctagta atctttgagc
     3541 cttgatacac tcttctccac tgatagctgg ttttatatta ttttctatac aatcaataaa
     3601 atgatttgat atatcctcaa aacccttttg ttttaatata tttacccatg caccactttg
     3661 ctttaaagtt aggtttccac cttcttctat ttccagtaca ttaagattct taactctttg
     3721 aatcttaccc tttgaaagaa tttcaacttg ttctaacttt gttccagaat ctctatgcat
     3781 agaggtgttt atagagaaat ttttaccttt aagtttatga cttacaaata taagattctt
     3841 attgtctgtt aaaaataaat cctctccact aatttcaaca tcttcatttg ctaaccataa
     3901 agcagtgtca ataacatgta tatagtcatc tataagagta ctgtcgaatt taacatttct
     3961 taaggaattt aatccatgtt tacatatatt gatagataca atttctgtag cattattttt
     4021 aatttctttg tacataggac agaatctacg gttgaatcca accattaaat ttaagttttt
     4081 tttcgtactt aattcgataa gttcttctcc ttgacttact gttgaagcaa gaggtttgtc
     4141 tacatataca tgaacaccta aatttaataa tatttttata atttcgtagt gtgtttctgt
     4201 agagctatgt agaaaaatac aatcacattt tttagctagg gattcaatag aatcgaattg
     4261 catgattcta taatcactac agactttttc ccttttaact ttatttggag taaaagctcc
     4321 tacaaactca aatctctcag acttagttaa tataggtaaa tatgcctttt gagctatgga
     4381 gccaagacct atcattccca tttttatatt tttcataata ctttctccta aggtttttat
     4441 atttacagaa ttgattataa ttcagacaca taaatttgta aactttgtat caaaaaataa
     4501 atttaaagct tatataagta aattagcgaa ataagaaata ttgataaagt aattttgtag
     4561 gatatctata aaattgattg atataaatat tttatgtatt gacaaaaaat tcaaattaat
     4621 ttatatttgg attagtggta attaccactt tggtctgaac aagtttttat cgtaaaataa
     4681 aaatactaaa gtatatagag tatgatttta ttaaattttt aaagataaac tataaaatta
     4741 attaaaatct atagtgtata ttttatagtg agtttaagta gtactaaaaa tctaagcaaa
     4801 aaattataaa agagtatatt tttttatagt ttaaagtggt tataacctca ttaccaaatg
     4861 taaacgttac aagtatgaaa ggagaatgaa aatgtttaaa ttagaagaac aaaaactaaa
     4921 agatttaggt gcaataataa caactaatga aataaaacaa cagcctgaat tatggttaga
     4981 gacttatgaa atatataaat ctaacaaaga aaaattaagt agattcatag atacaataag
     5041 taataatcat gggcagttta gagttatatt tacaggtgct ggaacttcag cttacatagg
     5101 aaattcaata cttccttacc ttaaaaataa aaatgacata agaaaataca tatttgaagc
     5161 aatacctact acggatatag tttcaaaccc atatgattac ttaaaaaaag atataccaac
     5221 attattaatt tcatttgcaa gaagtggaaa tagtcctgaa agtttggctg ctttaaattt
     5281 agggaataag attgtagata atttttatca tttagctata acttgtaatc cagaaggaga
     5341 actagctaag atgactaaaa acgacgaaaa taattacttg ttattaatgc catcaaaatc
     5401 aaatgatgaa ggctttgcca tgacaggaag tttttcgtgt atgatgttat ctgctatgtt
     5461 aatattcgat agtttagaag atgatgtaga aaagtcatat ataaatgcaa tagttgaaat
     5521 gggaagaaat gttatagata gaaaagatga aatacatgag ttaataaata aagattttga
     5581 tagagttgta tatttaggtt caggaggatt aggtggtttg acacaagaag cacaactaaa
     5641 acttttagaa cttacagcag gaaaaatatc tacagtgtat gattctccta tggggtttag
     5701 acacggacca aaatcattta ttgatgaaaa tacacttgta tttgaatttg tatctaactg
     5761 tttatataca agaaaatatg atttagatgt tttagaggag ataaaaaggg ataaaattgc
     5821 taaattcact tgtgcagtgt cagtagaaaa tgaaaataac ttctcaggaa caaagtttga
     5881 gtttaaagaa aagtataata aattaccaga tgtgtatctt gcaatgccat acatattgtt
     5941 tgcacaaact atagcattgt ttgtatcggt aaaggttgga aataagccag atacgccatc
     6001 agctacagga acagtaaatc gagtagtaaa aggtgtaaca atatatgaat attaaaagat
     6061 aagattaaaa tattataact aaataatttg ggagggtatc aatgtggcat tagtaacaac
     6121 aacatcaatg cttaaaaagg cacaagatga agggtatgca gtaggagcat ttaatgtaga
     6181 aaatatggaa atggtaattg ctgttataga agctgctgag gagttaaatt ctccagtaat
     6241 attacaaact actccatcta ctgtaaaata tgcaggtctt gattattatc tagcaaatgc
     6301 aaaggttgct gcagaaagag taaagatacc tgtagcaatg catctggacc atggttcaag
     6361 ttttgacctt gcaatgaaag ctttaagaag tggatataca tcaataatga tagatggttc
     6421 tcatgaaagc tttgaaaata atatatctat atctaaatca gtagtaaaag catgtagtcc
     6481 ttctcttata ccagtggaag cagaacttgg gaaagttggt ggaaaagaag atgatttaga
     6541 tggcgggaat agtaatgatt atacaaatcc aaaagaggca aaagagtttg tcgatagaac
     6601 aggaatatct tcattggcta ttgctatagg tactgcacat ggattgtata agggtgaacc
     6661 taaaatagat ttagataggt tatcagaaat taaaaatgta gtttcaatac ctttagtatt
     6721 gcatggaggt tcaggaatac atgatgatat tataaaagaa gctataaaaa gaggtattgc
     6781 aaaagttaac tatgcaactg aattaagaat tgcatatagt aaaggggtta aaaaagtttt
     6841 agatgaagat tctgaggtta tagaccctaa aaagtatgga ttagcagggc ttcaatcagt
     6901 aaaggatttt gtgaaagaaa agattaaagt gtgtgggtct gtaaatagag tataaaaata
     6961 aaatatccta aatttatttt agataagttt aggatatttg ttatatgaaa aatttatatt
     7021 aattatatgt aaatggtgta tttttttcat ttaattgatg aaaaatatca gcttttattg
     7081 gtaaagtatt acatatataa ttaaaatata ctaaaaattg aactatatat tttattatta
     7141 taaaatattt atattaaatt gtggaattaa aatatactag gatataaaat tcttaaataa
     7201 aaagaaaaga ttgtttttat aaaaaatatt ttttatacca cttaaaaaaa cacaataata
     7261 acactgaggg aggtggggat atgagagaac gcattattct tgaaacatta aaaaatagta
     7321 gtggttatct aaacatagat tattttgcag ataagttggg ggtatctact cgaactatca
     7381 gaaatgaaat aaaaaagata gaaactataa aagaatgtaa tggttttaaa ttagaatata
     7441 agtctaaatt aggatatatt ctaaatatta aagaccaaga taaattcgag cattatctaa
     7501 gaaacttacc ctcagattta gttgaaaatc cagaacaacg gttagagtct ataatagtag
     7561 aacttttagt aaatgaaggt tataaaacta tagaacagtt aagtaaaaaa tttctagtaa
     7621 gttcatccca aataaaaaat gatttaaaaa agatagatga aaagataaag gatacagaat
     7681 taaaactaga gcgaaaggct cattatggaa ttaaaataga aggttctata aaagaaattc
     7741 aaagtatatt ggtggatagt tattttagag ggaatagaaa tataacagag tacaggaata
     7801 agtttataga taatttaaaa ttagctaata ttagaagtac aataaaaaat gtacttaatg
     7861 aacatgattt agaagcaaat ttaacagaac ttgaggaaat attagcacaa attattatat
     7921 tgtatataag agttagtatg agagtagcgg gaaatgctag cgatttaaaa ttatatacag
     7981 aagatttaat tatagataaa ttattagata aaatttttag agataaaaaa tataacctta
     8041 attatgatga aaagtattat ttaaaggagc ttataaaact aaagactaaa gataagaaag
     8101 ccactataaa gaatattgat aagaataaac ttcaagatat tatgttcgaa ttttttagag
     8161 agatagataa aaaatataac tctaattttt tagaagataa agagttcttt aacttatttt
     8221 acttacatat tgcatgttta atagaacgta taaagaagaa ccacaagata ataaatcctt
     8281 tttcagttaa aataagtcaa cagtatccaa ctgtttttaa tttatctatt caattttcaa
     8341 aaatcataga aaatgagtat catataaaaa taagtcaaga tgaaataggg tttatagcta
     8401 ctcacattgc tgttcctttt gaaaagagag aagaagctaa ttttaataaa aaatataaaa
     8461 tagcaataat atgctcttct ggtggaggta gtgcattttt gataaattta agacttaaag
     8521 aaatttttcc aaatgctgag ataagaaatt tttcattact agaagaaaaa gctgtaatag
     8581 aatttgcacc agatttgatt ttttcaataa caaatctttt atttgaaata aatgctccag
     8641 ttatacttat aaatgaaatt ttagacgaat tggattattt agatattaaa gaaagtgtta
     8701 gatttgcaga ccatatagga aatatatcaa gcccaaaaca atatatatta ggtctatttg
     8761 ataagaatca ctttaggtgt ataaaagata aagctaagta taaggatatc cttgatagta
     8821 tgtcaaaatg tatagttgac gagggcgctt gttctccaac ctatcccaaa gatgtatggg
     8881 aaagagaaag ctatttgagt actatttata caaatggggt ggctatacct catccaatcg
     8941 agatgacagg aaacaaaaat ataatatcag tagctttgat acaaactgat attatttatg
     9001 aaaatagggt gcctaaaatt attttcatga tttcattaat aaagggaaat ttagaacttc
     9061 ataagcaaat atctaaatat ttaagtaaga ttatggcaaa taaggatatg gtagacatgc
     9121 taaataagtc acaatcttat gaagagttta tgtataagtt aaaaatctat ctgggaggtt
     9181 agacatgaat atagagtttt ttaagaggtt gtcagaagca gatggtatag catctaatga
     9241 agaagaggtt agacatgttt tattggaaga actaaaagct tatagtgata aaattatctg
     9301 tgatgggcta gggagtatca tcttttctaa aataaaagat gagagtgcac caaacgtaat
     9361 gatttgtgct catatggatg aagttggatt tatggtgaga agtatagaca aacttggtat
     9421 gattcactta ataacaatag gaggagtaaa acctcttgca cagtttgtac aaaaagttag
     9481 aattacaaca aaagaaggga agaaaatccc tgctgtcata aatgctacat ataataatgg
     9541 taaggctgaa aatatatatg cagatattgg agcatataca gaagaagatg tatataacct
     9601 agggattaat gttggggata tggtaacata tacaacaagc tttgaggaat ttactttacc
     9661 agaccgatta gtgggaaagg cttttgatga taggatagga tgttttgtga tgggagaagt
     9721 tttaaaagaa cttaggaaag aaaatctaaa ttgtaatatt cactttgcgg ctacaagtag
     9781 tgaagaggtt ggaataaggg gagcaaagac atcaacacag ttaataaatc cagatatagt
     9841 atttgtaata gatgtagcat gtgctaaaaa tgagtttgtg agagactata caaatcagaa
     9901 acaaattgat aaaggtgtaa tgctaatgca tagagatagg actctagtcc ctagtagaaa
     9961 aatgatagat tatgtaatgg aaattgctga taaaaataat ataccacttc aacatgatat
    10021 gtttgaatca ggtgggactg atggaggaga agcacatctc gtaaatgaag ggaaaccttg
    10081 tgtagtaact tgtgtaccag ttagatatgg tcattgtgca ttttcaatag taagtaataa
    10141 agatttagag aacattataa gattgtatac acagttaatt ttaaattttg atgaagaaca
    10201 atataaacat ataagaaatt ttatctaaag gagaataaga aaatgtcaac agaagtacaa
    10261 ataatggaac ttatatcaac tgcaggagag agtaaatcga aagcttttga ggctttaaaa
    10321 aaagtgaagt caaaagattt taaaggagca aaattgttaa tagaggaggc tcgtaaaata
    10381 gatattgaag ctcacaggat acaaacagaa ttaataactc aagatttaaa tgaagatgtt
    10441 gaaagtccag caatgagtct tttaatggta catgctcaag accactatat gacttctcag
    10501 ctagcaaaag acttgataga ggctttgata tattgtcttg ataagtaatt gtaatataga
    10561 aatagattat ttatgtaaaa attcttttaa aagtttagat gcttttaaga gataataaaa
    10621 atacaaactt taggaggaat tattatgaaa atattattat gttgtgcagg tggattttct
    10681 actaatatgt taatgcaaaa tatgaaaaag gtggtaaaag agagtgctaa actcaatgaa
    10741 aaagattttg agtttacagc tataccagca gattcaatag aaagtgaagt tgatgattgg
    10801 gatatagtac ttataggtcc tcaaatagct cacaaaatag acttcttaaa aggaatatta
    10861 gagccaaaaa ataagccttt tgcagtaata gataaagatg tatatggaaa aatggatgga
    10921 gctacagtta tgaagttggc attaataact tataagaaac atcaattagc tcaaggataa
    10981 taaaaataat gagggaaaag ggagattaga tatgttttca aaatttgaag catttatgaa
    11041 tagagtattt atgcctttag ctcataaggt tgacaatcaa cgtcaccttg gttctataaa
    11101 ggctggtatg gttgctatga caccattcac aatattagga agtatatttg caatacttcc
    11161 agcacttcca aatatgcttg gagaaaataa tccaataagc aaattcatac ttaacaattc
    11221 agcaatattg gatttaccag taagtctttc cataggttta ataggattat atgcttgtat
    11281 gtgtattgct tttaatctgg ggaactacta taaattatat ataccaggtt gtgttgcact
    11341 atcaactttt tcttttttat tcctagtagc atcatttaca ccagatggag ctttagaaat
    11401 aggaaattta ggagctaaag ggctatttac aggtatgata gcagggcttg gttctgtgga
    11461 gttatataat ttttgtaata agaagaatat cacaataaaa atgccagaaa atgtacctga
    11521 ttttgtatct aggtcttttg aattaatacc tactactata atagtttgtg gaacttttat
    11581 agttgctcgt tttatttcat taaaagtatt tggagaatta cctccacaga tacttactca
    11641 atttttatct ccattagtag gaagtatgga taacccatgg tcagttcttg cacttaactt
    11701 tgctatatgt actatattct tttttggaat acattcatca gtatttagtc ctataactag
    11761 acctataatg attacattta tagctgaaaa catagcagct atgcaagctg gagaaacaat
    11821 acctcatttc tttacagcag gagcttcaag tgcattcttt ggatttacag gatgtggaat
    11881 tagtataggt tgtgtaattg catgtatgtt atcaaaaaat gcttcctata agaagattgg
    11941 acgtgtatca ttattccctg cattatttgg gataaatgaa ccaatattat ttggagcacc
    12001 gataatatta aatccaataa tgtttatacc acatgtattt ggtggagcta taataggtac
    12061 actaccaatg ttttttatgc actggggatg gttagcaaag ccagtatttg accctccata
    12121 tgtaggggta ttcttagaag gatttttaac taatggagat tggcattcta tattagcaaa
    12181 tgcattgcaa ttagtattag cagttttatt atattggcca ttctttaaaa taatggaacg
    12241 tcaacaaaat aataaaaaac aaaaagaaga aactaagaat atattctcta aagaagaaga
    12301 agacttattg gatgatttag gattggattt ctaatatttt gttatgaata atacaatgga
    12361 gagattagta tccttaatct ataaaagagg tgttgacgct cttttgatta aagcaaaaaa
    12421 tactaaaaga tatataggag ctttatcagg tagtggtgta tatgtgcttg ttactaagga
    12481 taaaaattat caaattctag atggcagata tattgatgaa gcagataaaa aaacgactgg
    12541 tttcataaaa aggattgtat ctcaaggttc ttatatacca actataattg agttattaaa
    12601 agaattaaat ataaaaaagt tagcaataga aaaccaagct atgagtatac aagagtatct
    12661 ttcactaaaa aatgaaggtt ttgaaatcaa tttaataaca aatgaattat ggaagctaag
    12721 ggctataaaa tctaaagaag aaattgaact tattaaaaag gcttgtgaaa ttacagatga
    12781 agtatttgat gaggttattt ctgaaattaa aaaagatatg actgagctag aagtatcagc
    12841 tctaattcag tatcatgcat taaaaaaagg tgcaagtgga atgtcttttg aaacaatagt
    12901 ggtatcagga gaaagaggtg caatgccaca tggaagacct acaaacaaaa aattgaaagt
    12961 gaatgaagct ataacaatag attttggtgt tgtttatcaa gggtatcaat cagacatgac
    13021 tcgtacaata tctataggta aacctcctaa aattattaaa gagatatatg atgtagtatt
    13081 ggaagcacaa ttatctgcaa tagagtcaat aaaggaagga actagggcaa gtgatgttga
    13141 caaagtggct agaaaaatta tagataaaca tggatttggc gaatatttta atcatggact
    13201 gggtcatggt ataggtcttg gagatggtga agtccccact ttaaatccaa atagtgaaga
    13261 tatattagtg gaaggaatgg taatgtcttg tgagccaggt atttatattc caaatgtagg
    13321 gggagttaga atagaagatg acattgttat aatagatgga aaaggtgttc cactcaataa
    13381 gacatccaaa gagtttataa tattaggaga gtaaaattat gaaatataat tttaataaag
    13441 tattaaatcg taaagaagga aattgtagaa aatggagcaa ccatgtgatt aaagaaaaat
    13501 tcggcttaaa tgaagatgca attcctatgg atttagctga tatcgatttt gagtgtgctc
    13561 ctgctataaa agaatctatc ttaaaaagag catcagtagg agattatagt tatactttta
    13621 taggagatga cttctatgat gcgattataa attggaataa aagaagattt gatgtatata
    13681 tagaaaagga ttggataaaa ttaacttttg gtactgtttc aacccttcat tatatagtcc
    13741 aagcatatac taaagaagga gagggtgtat tgataaatac acctgcttat gacccttttg
    13801 cagaggctgt agtaaataat aatagaaagt tgtgttgtag ctcattaaaa ttagataaca
    13861 atagatatta tttagattta gaggatatgg aaaatcaaat gaaatacgag aatataagat
    13921 tgcttatatt ctgtagtcca caaaatccat caggtcgtat atggacaaaa gaagaactat
    13981 atcaagtgtc agagttatgt ttaaagtata atgtaatttt ggtaagtgat gaaattcata
    14041 gagatataat atttaaagat tatagatttg tatctttatg gaatgctcat ccagaaattt
    14101 ataaaaatag tataatctgt gtatctccta ataaaggctt caacttaggt ggattaaaaa
    14161 catcatatgt attaataaga gatgtaaaaa ttagggagac tttattagaa agactaaaaa
    14221 gtaattcaat aacttctcca aatgtatttg caataccagc aatagttgca gcatataatg
    14281 aaagcgaaga atggctagat gctatgacta gttatgtaga ggagaatttt gaaatagttt
    14341 atgatttctt tgaaactaat ataccaaaag ctaaagtgat gaagtcagac tcttcttttt
    14401 tagcctggat agatgtaaga gaagtattta gaaatgaaga agaatctaaa gaatttttta
    14461 gacatgcaaa cttaactatg gtagttggaa gttattttgt gaaagatgga gatggattta
    14521 ttcgtattaa tgtggggtgt cctagagaaa cattaaatga ggctctaaat agaatcaaaa
    14581 aaacgtatat aagtatgtat tgttaaatca taaaaaaagt atctatggtt tatataaaaa
    14641 cacattattt agttatcaaa tattactgga attttgtatt ccagtaatat tttttgatta
    14701 aggaatatta ttgaggaata ttaaataata ttatttcata cccttatatt aacttatata
    14761 aaaatatttt attattttgg gaacgggtta gctaaccttt atctagtgta tatttatatt
    14821 gtaagaagca gtaaaaactt ttcctttact aagtttatta ataaacttct tatctttata
    14881 aattttgtta gaatttatac tatcagcaag accataccaa ggttctatac ataaaaaagg
    14941 agcagtagaa ttagtttcac tataataagg agtccaaata ccaactagag ggaaatcttc
    15001 catagatata gttacatatt tactaccatc attattacat atagatactt catctatgtt
    15061 ggtgtatatc aaagcatcat tcttgaatac ttctggattt aactgtaagt tttttaattt
    15121 tccaataggt attatatcat ctgtaaaaga accatttaaa ttgatttttt ctacatcatt
    15181 tttactttta aactcaaggt agtgtttaga aaaatcattt tgttcgtaaa aaggaaggtt
    15241 gaatgcagga tgtccaccta tagaaaagaa tatatcttta gaatcagtat tttttacaat
    15301 ccattctacc tcaatactat tatcatttaa tgtatagttt atgattagct caaaagaata
    15361 tggatacttc tttaaagtag attcattatc tgttaattta taagttacaa atgtatctcc
    15421 tttatctaca atctcaaagt ccatatctct agcaaaccca tgttgagtca tgctatataa
    15481 attttcttct attattgttt cattatcaaa aagtttacct actataggga acaatatagg
    15541 tgattgtcta ccccaatatt ttttattacc atcccaaaga atgtttttgt cgtatttttt
    15601 agaaaatatt ttagttagtt cagcaccaaa actgcttgat tcgataatta aattttcatt
    15661 ttgtaatata ttcatttaaa atctccttta ttatatttta tgagtatatt atactataaa
    15721 gtataaagat tattaaattt tatttgatat tatatttgaa aggtagattt tagtatattt
    15781 tgaaaatttt gttttgaaaa tttgggatta tttaagaaat tttgtattat aaatagaagg
    15841 aaatagataa attgtataga ataaaatata ttaagataag ttaataatgt taaatgggaa
    15901 gtgagtttat ggaaaaacca aatacaaaag ttgttaactt tcaagagtat aaagacagaa
    15961 aaaaccaaag agaagtaaat agagacgaac ttttaaagct tataaaaaaa attgtgactt
    16021 caaaataggg agttgttaaa ctccctattt taatgggttt cataataatt aagacattct
    16081 gagtccatct atataatctt tgaattgtga gaaatctgat tcactactta attctatggt
    16141 ttctgaacct tccgtataaa aattcttaag atagatagta taattattat caacacaatc
    16201 atgataaata catctatatc catcttcata caaacttttt aacttagaaa aatcatccac
    16261 aatatcatct ccttaaagtt cttttaaata tatttttaca aaaagcatct aattgtatgc
    16321 aaattgacaa tgtaaaataa aggaaatgaa cataaaatgt agaataattt ttatatgtat
    16381 gtaaaaataa aaagaaaaag ttgaaatttt tagaaaataa gagtattttt tatctaaaaa
    16441 gagagtttaa aaaataaaaa aatgttggga ggataaaaaa tgagtttatt agaaagtata
    16501 tcaaaaaaca tatattccct agataactct tctattgaaa aaacaaagca gagattggat
    16561 aggcttatac atccaactgg aagcttagga aagatagaag atatttgcat gcaattagca
    16621 ggaatatttg gcaatgaaaa ttttgacaca agtaaaaagg ttataattgc ttttgcggga
    16681 gaccatggtg tatatgaaga aggcgtagca cctgaccctc aaaatataac aaaattacag
    16741 tttccaaatt tttcgaaagg tctatgtgga gttggagtaa taagtaaatt tgtaggggca
    16801 gatgttgtag ctgtagatgt aggaataaac tgtgatgaaa aactggatgg ggtattagac
    16861 tacaagataa gaaaagggac atcaaatatg gcaaaaggtc cagctatgag taaacaagaa
    16921 gcaattagat gtttagagat aggtattgaa atagcagagc agtgtataga aaaagattat
    16981 aaggttattg gtattgggga gatgggtata gcaaatacta ctccaagtac agcaataata
    17041 tctgtaattt cagggtgtga cccgttagaa gttactggta taggtgcagg tcttaagaaa
    17101 gaaagactaa agcataaggc tgaagtaatt agaaaggcta ttgaaattaa taatccaaat
    17161 ccaactgatg gagtagatat tttatctaaa gtaggagggt ttgaaatagg ctcaatggca
    17221 ggtgttatat taggttgtag tgctaatagg attccagtag taattgatgg gtttatttct
    17281 tatgcagcag cattaattgc atataagata aatccaaaaa ctagagaata tatgatagca
    17341 tctcatttat cagcagaatc aggaactaaa agagcattag acatattaaa attagaccct
    17401 ctattaaata tggatatgag attaggtgag ggaagtggag ctgctttagc atttgatata
    17461 atagaagctt caaactatac ttataaaaat atggcaactt ttgatgaaat tgacatggga
    17521 agataaatat aaaaatttaa tatgtaggag tgttttgatg agtaaaatta ttctagtaac
    17581 aggaggagct aggtcaggga agagtaactt tgcagaaaaa ctatgttttg acagaaataa
    17641 taatacagct tatatagcaa cctctattcc atttgatgat gaaatgaaag atagagtaaa
    17701 aaaacatcaa gagaatagac ctagaaactg gaatacttat gaaatatatg aagatatata
    17761 ttccataatt aaggagatat ataaagaaca tgaaactgtt atactagatt gtgtaacatt
    17821 attagtaaat aatctaatgt ttgaacacaa tctaaatata gaagaatcta gtcaagaaga
    17881 aataaacaaa ttagaacaat acataaaaga acaagttgtc aaacttattg aagaaataga
    17941 aaaaacaaat ctatactttg tatttgtaac gaatgagtta ggtatgggaa ttgttcctgg
    18001 gaataaactt agtagaattt atgctgatat tgtaggtcgt ataaatcaat atatagcttc
    18061 gaaaagtaat gaagtttatt ttgtagttag tggcatagct atgaagataa aggagtagta
    18121 tgcaattatg aaaagattta ttttaatact acagtttttg acaaggatac ctataaaatt
    18181 aaatgtagga tttgatgatg aattttataa gtcaatagta tattttccac ttgtaggttt
    18241 tgtcataggt atattatcct atttaatagg atggatttct atgcttttat ttgaaccatt
    18301 tatagcttct ataataataa ctctagctgg tgtcttaatt actggaggac ttcatataga
    18361 tggattagga gatacatttg atgcaatata tagttatagg gataaagaaa agatgcttga
    18421 aataatgaaa gattcaagat taggtacaaa ttcacttcta gcaattatgt ttgtactttt
    18481 attaaaggtt ggattcgttt acgatataat aagtaataat tcattgtggg ttataatttt
    18541 tatgccaatg attgcaaggt taggagtaat gttattgaca tataaaactg tgacaccaag
    18601 agaaaaagga atgggaaatt tatttatagg aaaattaact acaagtatgt taataactgc
    18661 tataatatac acattattga tagttgctct tattactaaa tttatatttt tattacctaa
    18721 tatagtgttg attaaagtat taggttcgat tatagtggtt tttgtattta taatattatt
    18781 caaaaaacat atttataaaa aaattgacgg agtaacagga gatatattag gctgtggaat
    18841 agaactttca gagcttgtat atttaattta catatatttg ttaattttta tgttttttta
    18901 aagtaaaaag tggtgataat atgataagat taatattaat aagacatgca ctgacaaatg
    18961 ataataaaaa aggaagatta tcaggccata taaatagttg tattagtgaa gagggcaaac
    19021 tacaaataaa taaaattacc agatatctga gtaatgaaaa catagataag gtttatacaa
    19081 caccatctac acgtactaaa gatacagtag aaaaaatatc aaaattgaaa ttattagaaa
    19141 tagaagaaaa agaagcttta agagaaatta gttttggaga ctttgaagga agaacttttg
    19201 aagaaattaa aatcaaatat ccaaatgagt ttgaaaaaat gataaaagaa ggtaacaact
    19261 atagatatcc aaatggagaa agtttgatag actcttataa aagagtggca aaagaaattg
    19321 ataatataat tttagaaaat gatagtaatt tagatacaaa gacaatactt atatgttctc
    19381 atgcaggaac aataagaaat ataataacac atttgatttc tggtagttat aagtatcact
    19441 ggaactttaa aattgataat gcatctatta cagttttaga aattgatggt ggatttgctg
    19501 taatagataa aatgaatttt acagacttca tataatttaa tcaaaaccaa acattacaat
    19561 ctgtgtttgt aatatatctg taaaattagc aatgaaaata gaatattaga ttaaaattag
    19621 gttcttaatt agatgttaaa agggaaaaag gttagatgcc tttgcagccc ccgctactgt
    19681 gaaaccaacg aaactataaa taccactgtc agtttgatgg gaaggttata gaagtaggat
    19741 gaagttaagc caggatacct gcctaattta atttaaaaca agaggtcttc ggggtgaaag
    19801 atttttaatt aatatatata aaagtcctat ctttagaagt attaggactt tttttaattt
    19861 gttttttagt aaattttaac aaggagggat ggaaagtggg gaaaaaaata atgctacaag
    19921 gaacagcttc aaatgtaggt aaaagtgtct tagtagcagg tttgtgtaga atatttaagc
    19981 aagatggtta tactgttgct ccttttaagt cacaaaatat ggctttaaat tcttttataa
    20041 ctaaagaagg attagaaatg ggaagagcac aagtatttca agcagaggct gcgaaaatag
    20101 agccaatagc tgatatgaat ccagttttat taaaaccttc tggaaataat aaaagtcaag
    20161 ttattgtaag aggtaaagtt gtaggagaaa tgccttcttc taagtatcat gattataaat
    20221 tggagttagt agatgtgttg aaggaaacat ttaatagctt aaattcaagg tatgatattg
    20281 tagttatgga aggggcagga agtaatgcag agataaactt aaaagataga gatatctcta
    20341 atatgggtat ggcagaaata gctgatgcac ctgtaataat agtcggagat attgatagag
    20401 gaggcgtatt tgcctcatta gcaggaacaa tgcttttatt aaatgaagat gagaaaagaa
    20461 gagtaaaagg tgtaataatc aataaattca gaggaaaaaa agagctttta caagatggtg
    20521 taaagatgct agaagacatc ataaaagtac ctgttatagg agtagtacct tatacagata
    20581 taaagataga agatgaagac agtgtaacta cgagatttaa gaaaaaaatg aataaaagtg
    20641 atatacacat agaaataatt agaactccac atatgtcaaa ttttacagat tttaatatat
    20701 ttgagacaca agaagatgta agtattagat atgtagacta tggagaatct ttaggaaatc
    20761 cagatatatt aataatacct ggaactaaaa gtactataga cgatttaaca tatataagaa
    20821 aaagtggtct tgaatcgcaa atcaaaaatt tacatagtca aggtaaattg atatttggga
    20881 tatgtggtgg ataccaaatg ttaggtaaaa aattaaaaga cccttatcat gttgaaagtg
    20941 aaattgaaga agttgatgga ataggtcttt tagatacaga aacaacgttt gagaaagaaa
    21001 agactacaac acaagtagaa gctactattg ttgaatgtat tagtgaatac atgaataacc
    21061 ttaagggaaa aaaagtaaaa ggttatgaaa ttcatatggg aattacaaag cgaggtaatg
    21121 atgtaagaag tttagattta ataactaaaa aacttgataa agaagtgaat tatactgagg
    21181 gaagtgtaaa taaagagggg aatgtaattg ggacttatct tcatggaata tttgatgaaa
    21241 taagctttac aagagcaatt ttaaacaata taagaaagaa aaaaggttta ggagaattag
    21301 aaagcaaagt aaactctttt aatgaattta aggataaaga gtatgataag ttggctgatt
    21361 ttttaagaga acatttagat atagagaaaa tatatgagat tatgaaataa tgatgacctt
    21421 tatttataat ttaggtagga ggtgtattat gaaaaaaatt ttaattgcgg gtactaatag
    21481 tggagtaggt aaaacaacaa tatcattagg aataatgcaa gctttggtta agagaaatat
    21541 gaaagtacag ccatataaag tagggccaga ctatatagac ccttcatatc acacttttat
    21601 aactggaaga cattctagaa atttagattc ttatatgtta gatgatgaaa aaataaagta
    21661 tatagtaaat aagtcctcaa aagatgcaga tatttcagta atagaaggtg ttatgggctt
    21721 atatgatggc tttggaatag atttagataa ttgtacaagt tcacatactt caaaggtatt
    21781 aaagtcacct gttatattag ttataaatgg taaatctatg gcagcatcta gtgctgctat
    21841 ggtacttgga tataaggaac tagataaaga tgtaaatata aagggtgtta tagtaaataa
    21901 tgtaaagact aatagccatt atcaaataat taagagttct atagaaaaat attgtaatgt
    21961 tgaagtattg gggtattttc ctccaaataa caaattctct ttggaatctc gacatctagg
    22021 attagttcct agtgtagaaa tgaaatcact aagggaaaaa ttttatacct tagcagatga
    22081 aattgaacac tatatagata ttgacagaat aattgaaata tcagaaagcg atgaatttga
    22141 gagtgatttt gattttcaaa ctttaataga aaataataaa ataaaagaac atgttaataa
    22201 taaatcaata gctatagcat atgataaagc attcaatttt tattatagag aaaatataga
    22261 gcttttagag gaacttggtt tagaaataaa ctattttagc cctcttgaag atgtatcagt
    22321 gccaaatgca gattatattt atattggagg aggttttcca gaaatatttg gaaaagaatt
    22381 agaagcaaat gagtcaatga gaacttccat attagaagca cataacaata atatacctat
    22441 ttatgcagaa tgtggaggac tgatgtacct aggtgagaaa ctattgaatc aagaagaaca
    22501 agaatttaat atggttggaa tatttagtgg aattagtaag atgacatctt ctttaaaaag
    22561 atttgggtac tgtattggtg tagccaagga aaatactctt ttatctaaaa aaggggaatc
    22621 tattaaaggg catgagtttc atcattcaat atttgaaagt gatgaaaaat gtgtatattc
    22681 aatgagaaaa gaaagagatg gaaatatagt agaagagtgg gatggtggtt atagtaaagg
    22741 aaacactttg gctacatatc ttcatactca tttttataat aacttaaatt gtatagaaaa
    22801 ttttttgaaa aaaggaaatg aataaatgag tattttatct atttatatag gatatgtgac
    22861 agacttaatt gtaggagacc catattcttt tcctcatcca gtgagattta ttggtaaact
    22921 tataaatttt actcaaggta taataagaaa ggtctctaaa aatgataggc agttaaaatt
    22981 ggggggattt atattatggt ttataaccgt tggaataaca tatttaacaa cttatgctat
    23041 agtgaagtta tttagtttta atatattatt gagtattgta gtgaatagtt ttataattta
    23101 tacaacacta gccacaaaat gtttaaaaga cgaagcatta aaaatttata atgtattaaa
    23161 aactgaagat atagaaaaat ctaggattca gctatcttat atagtaggta gggatactac
    23221 taatttaagt gaacctgaaa taataagagc aactgtagaa actgttgcag aaaatactgt
    23281 agatggtata attgcgccta tgttttatgc atttattgga ggagcaccac ttgctatggc
    23341 ctataaagct atcaatacat tggactcaac agttggatat aaaaataaaa agtataaaga
    23401 tattggtttt gcatctgcta agattgatga tatagccaat tatataccag ctagaatatc
    23461 tgtcattctt atgacaattg gaagtttctt tttaaattat aactatagga actgttttaa
    23521 aatctctatt agagatagaa agaatcataa aagtccaaat tgtgcttttt cagaaggtgc
    23581 agtatctggt gctctaggaa tacaactagg aggtaccaac gtttattttg gggaaaaagt
    23641 atataagcca acaatagggg ataagcttag ggagatagat aaagaagata taattaaaac
    23701 taataaaatt atgtatgcat cttcatttgt atctatgttg gtatttacac taatatatgt
    23761 gttatgtagg atgattttaa ttaacttaat tatataaatt attgtttgtt aacttaaggg
    23821 ggacatagat gaaggattta gggcatggtg ccaatgtaga tgaaatggca agattatatg
    23881 ggaaaaaccc aaaagaaata attgatttta gttcaaacat aaatccaaat gtattaccaa
    23941 atttagagag atacatttta aaaggattag aagagtgtag gaattatcca gatataaatt
    24001 atacaaactt gagagaaaat atatcaaaat atatagatat aaatccagac tttataatac
    24061 ctggcaatgg tgcaacagaa gtgatttatt tgcttatgaa gagtattaaa aaaaaattgg
    24121 ctataataaa tccgactttt tcagagtata gaagaagtgc tgagttaaat aatttagata
    24181 ttatagattt agagttagat ttagaaaata actttaaact taatatagat ataataaaag
    24241 aaaatataaa aagatttgat agtttattta tatgtaatcc taataatccg agtggtaatg
    24301 tccaagattt aaaggaactt gttcatttac tagataaaca taataaggta ttaataattg
    24361 atgaaacttt catggaattt gttgaagatg aaagtaaata tagtttggtt aaatatatag
    24421 aatcaaataa aaatatattt ataattaaag ctgtgactaa gttttttggt atgcctggtt
    24481 taaggctagg atatggtctt acaagcaaca ctgaaattat gaacaaaatt tatgaacaca
    24541 aagagccatg gactatcaat tcttttgcag atatactatc caattttatt tttgaagata
    24601 aagagtatat taaaaatagt aaagaatatt acatagaaga aagagaatat atgttacaag
    24661 agttgagaaa cataagaaat ataaaggtct atgatacaga tgctaatttt atcttgataa
    24721 aaatatataa aaaaacaact aaagaattga aaaaagattt gtttaagcaa ggaaacatat
    24781 tagttagaga tgcgtctaat tttataggat tagatgatag ttttataaga gtggctataa
    24841 aatctcatga agataacaaa attcttatag aaaatatgaa aaatttattg ggtgattaaa
    24901 atgaaatctt atggaatatg cccagcatct tgtggagaat ttgtacaagg tataattgat
    24961 gatgaagaat atttatgttc atatgcaata gatatgtatt ctaaagtgta tattgaagaa
    25021 aaattagtag atataaattt agggagatat aagtcaaggt tagcaattga aaaggtattt
    25081 gaaaaattta atttacctaa aaaatatact aaaaatattt ctttaaatat aaatagtaaa
    25141 atacctgttg gaaaaggaat ggcaagttca actgctgata taggagctac tataaaggca
    25201 acattatcat tgatagataa agatttaagt agcgaggaga tttctaaatt agcagcagaa
    25261 atagaaccaa cagattctat atttatagat aaaaatagta tatttaatcc tctaaatgga
    25321 actgttataa aatacctagg aaatctgacc aatgcgaaag ttgtaatact tgaaccaaac
    25381 aaagtactag atactatgaa aattagattg aggcaagatt ataataaatt aaaagtagag
    25441 aataaagaag taataaaaaa atcatttgct cttttagaag aaggattgaa aaaaaataat
    25501 ttatctctag ttggagaggc ttgtactttg agtagcttag caaatgagaa tatagaaaaa
    25561 aaagagtatt taaatgaaat aataaaaata tcaaaaaaat atggagcata tggagtaaat
    25621 attgctcaca gtggaactgt agtaggtatt ttaattgata agtttatgaa tgataaaaag
    25681 atgatagatg ccttatgtga atctaacata aattctgtct ataataaaat ttatacacaa
    25741 aacataatta atggaggaat taagggggaa atagaatgga atacataaag aatcctatga
    25801 aaatagaaga aaaaagtttt gaattgatac aagaaataat agacgaaatt agaccagatt
    25861 ataaatttaa gaataatatc gaagaaaaaa taataaaaag agctatacat actactgctg
    25921 attttgatta cttagatata ttaaaaatat cagaagaagc agttgatagt ataatagatg
    25981 cattaaaaaa taatgcaagt atttatactg atacaaatat ggctcttagt ggtataaata
    26041 aaagaaagtt agaatcatta ggatgtaagt ataaatgttt agtggcagat gatgaaactg
    26101 taaaattagc taaagaaaaa ggaattactc gttctatggc agcagtagaa attgcagcta
    26161 aagaagaagg aagaaaaata ttcgttttag gtaatgctcc aacagcttta tataaagtta
    26221 tggagatgaa atcagaaggt agattagatt tagatgcagt tataggagtt cctgtaggtt
    26281 ttgtaggagc acaagaatca aaagatgaag ttgaaaaaac agatatacca tatataattt
    26341 ctaaaggtag aaaaggtggc agtaatttag cagcagcaat agtaaatgct atattatata
    26401 ctatgtaggt acctatatgg aagaatatgt atatatagat ggaaaaaaat atagaagagg
    26461 atatactaca ggttcatgtg caacaggtgc atctaaggct gctgtatata tgttgattac
    26521 aaaaaataga attaatacta taaatataga tactcctaag gggataccat tactactaaa
    26581 agttgataat ataaatattt ctgatacatt tgtggaatgt tccataaaaa aagatggtgg
    26641 agatgatata gatgcaactc ataccatgga tatttatgca agagctgaaa ttgtagctaa
    26701 aaatgataag aacaaagggt atcttactct aaaggatata gatagtttaa gcaccaatag
    26761 tgagtgtaaa agtgagctct ataagtttat tagggtttat ggcggaactg gtattggtgt
    26821 ggttacgaaa aaagggctta gtgtggatgt tggtaagcct gctataaatc cgactccact
    26881 taaaatgata aatcatgaaa taagaaagct tataggagat aattttgaat ctatacttgg
    26941 aaatgataaa gttttaaaaa taacaatctt tgctccacaa ggagaaactg tagcaaaaaa
    27001 aacatttaat ccaagattag gtatagttgg aggaatatct ataataggta caactgggat
    27061 tgttgaaccc atgagtgatg atggttggaa gaagtctcta tctatagaac ttcagatgaa
    27121 aaaagaacaa ggcttagata aaataatatt ggttccagga aatcatggag aacagtttat
    27181 aagagaaaaa ttaaacttag atattaagta tgtagttagg gtaagtaatt ttataggcta
    27241 tatgattaaa gaagctcaaa gaataggata taaaaaaatc ttaatggcag gccatatagg
    27301 taaatttata aaagtatctg ctggtatttt taatactcat agtaaggtag cagatgctag
    27361 aagtgagata ttagtggcaa acttagcttt aatgggagct agatacgaat ttttaaataa
    27421 aataaatcaa tgcgtaacta ctgaagaagc tgtagagcta ataaataata gtgaatatag
    27481 agaagtatat aatatattaa gtaataaatg tagggagaga gtaaagcaat acttgaatga
    27541 agattcagat gatattgatg tggaagtaat catattttca atggataagt ctttattagg
    27601 taaatcggat aatacagatg atttagtgga ggtctttata tgataaacat aataggtcta
    27661 ggacctggta acttagacta tataacaaaa aaaggtgaaa atttaatttc cacttctgat
    27721 gtattaattg gtggtaaaag aaatttagag tctataaaaa attttgaagg agaaaaaata
    27781 gttctggatt ctaatttaag agaaattata gagtacataa ataacaataa agaaaagcaa
    27841 atatcaataa tagcatctgg ggacccatta atatatggga taggtagata tctatctaaa
    27901 aatattgata ataagatgtt aaacatggtt tctgggatta gttcattaca gtacatattt
    27961 tcaaaaatat atgtagatat gaatgatgta tatattacaa gtagtcatgg caaagtacca
    28021 gattttgatt acatattatc acataaaaaa gtatgtatgg ttacagattc aaagatagga
    28081 ccaaaacaaa taagtagaga aataatagat agaaatctaa ataaaattat tgttgtagga
    28141 gaaaatctgt catatgacaa tgaaaaaata acaatagcaa aaccagaaga gataataaga
    28201 atggacaatt ttgatatgaa tgttgtggtg attttagatg aggaatagtg aatttataac
    28261 aggtaaagtt ccaattacta aggaagaggt tagagcaata tcaataagta agttaaattt
    28321 agtaaatgct aaaaacctca tagacatagg ggctggtaca ggaagtgtaa gtgttgaagc
    28381 tgcatataat tatcctaatt taaaggttat atctatagag aaaaatgatg atgctataga
    28441 gcttatagag aaaaataaaa aaaaattcaa cttaaaaaat atagaagtga taaaaggata
    28501 tgctccaatt aatctagggt taaatgttaa agttgagtct atttttcttg ggggaacagg
    28561 aaataatctg gaagaaataa tagaatggtc taaaaaaaat ctcgttacag gaggaagact
    28621 tgtagctaat tttataataa tagatacatt taatcaaact ttaaaactgc ttaaaaagca
    28681 tggatttaaa gaaatagatg tatgtgtttt gaatgtttct aaactagaga aactaggaaa
    28741 aggtgaatat tttaaaccac taaatcctat atatataata tcttgtgaaa aaggggaaaa
    28801 tgatgatgaa taaagtacat tttgtaggag caggacctgg agataaagag ctgataacat
    28861 taaaaggata caaattatta agtaatgcag atgtagtgat atatgcaggt tcacttgtta
    28921 atcctgaact tttggaatac tgtaaagaag attgccaaat acataatagt gcacatatgg
    28981 atttacaaga gataatagat gttatgagag aaggtataga aaataataaa tctgttgtaa
    29041 gactgcaaac aggtgatttt tctatatatg gttcaattag agaacaagtg gaagatttaa
    29101 ataaattgaa tatagattat gactgtactc caggagtaag ttctttttta ggggcagcat
    29161 catctttagg ggttgaatat acagttccag aaatttctca aagtgtaatt ataacaagaa
    29221 tggaaggcag aactccggtt ccagagaaag aatcaataca atcatatgct aagcatcaga
    29281 catctatggt aatattttta tctgttcaag aaattgagaa agttgtgtct aaattactgg
    29341 aaggtggata tccaaaagat actcctatag cagttatata caaagcaact tgggcagatg
    29401 aaaaaatagt taaaggaact ttaagtgata tagctgtaaa agttaaagaa aacaacataa
    29461 ataagactgc tttaataatg gttgggagat ttttaggaga agagtataat aattcaaagt
    29521 tatatgataa ggatttcaaa catgaatata gagggtaata ttgctataat ctgtattaca
    29581 gaaaatggaa aaaaattagc tctaaagata aattctctag taaataactg tgttatatat
    29641 catgtaaaaa ataaaaaaag taatttagtt gttgaagaaa catgtgtgca tacagttcaa
    29701 aagaagttaa gtgattttgt aggagaaata tttagcaaat ttgattacat agtatttata
    29761 atggctacag gtatagttgt acgaagtata gcaccataca taataagtaa gtttagtgac
    29821 cctgcaattt tggtaatgga tgaaaaaggc aaaaacgtaa taagtttatt gagtggtcat
    29881 attggagggg caaacagctt gacacagtac ataagtagtc ttattgatgc taatccagta
    29941 ataacaacag ctactgatat aaatcaaaaa gcttccttag atatgatagc aaaaaaacta
    30001 aatgcacata ttgataattt tagagaaagt gtaaaagaag taaattctat gctagtgaat
    30061 aataaatgtg ttggattata tattgatgaa ggatatcaag ttgatactag gggatttaaa
    30121 cttatagaaa gtaaggatga gttaagagat agtttttcaa acacatatat agaaaaatta
    30181 gttgtagtaa gcaataaaat aaattgtatt gaagatattg agaatttaaa cctagataat
    30241 aaatttgaac attgctatag taccaaaaaa ataataaagg taattccaag agatatagtg
    30301 ataggaattg gttgtagaag aaacacagat agtgaactga tgtataattc acttgaagag
    30361 tttcttaata aacaaaatat agatattaga gcaataaaca cagttggaag tatagaactg
    30421 aaaaaagacg aaaaagcaat aattgaatta tcaaaaatat tgaaggtgcc atttaaaaca
    30481 ttttcagtag atgaaatatc taaggttgat aatctttttg ataaatctga ttttgttaaa
    30541 aaaaatgtgg gcgtgtattc agtagcagaa cctgtagcat atcttttgag caaaggtaat
    30601 ttgttagttg aaaaacataa atataaagga ataacttttt cagtggggag aataaaaata
    30661 tgatatacgt tgtaggaatt ggaccaggaa gtaaagatac aatgacttta gaagcaataa
    30721 aagcaataga agattctgag gctattgttg gatataagac ttatataaaa ctaatagaag
    30781 aatttataca agataaagaa ataatccaaa atggtatgag gcaagaagtt gatagatgta
    30841 aacaagcagt agaggaagca aaaaaaggaa aaaaagtagc agttgtaagc agtggtgatg
    30901 ctggaatata tgggatggca ggtcttattt tagaattaat atctaaagaa gatgaagata
    30961 taaaagtaaa ggtaattcca ggtgttactg caagtattgg tgcagcagct atacttggag
    31021 ctcctattat gcatgatttc tgtcatataa gtttgagtga tttaatgact ccgtgggaag
    31081 taattgaaaa aagattgaga ttggccgctg aagccgattt tgtaatttgt ttgtataatc
    31141 caagaagtaa gggaagaagt gaacatctaa gcaaagcatt taaaataatg ggtgagttta
    31201 aaggtggaga tacacctgtt ggagtggtaa aagatgtagg tagagaaaaa gaagaaaagt
    31261 ttgtatgtac ttttgaaaat atggactttg aaaaagtcga tatgactaca atggttataa
    31321 taggaaataa gtctacctat attaatggtg aattgatgat aactccaaga ggttatacag
    31381 tatgatttta attttaggag gaacttcaga tagtttatct atatgtgata agataaatga
    31441 actgaaaaat caaccatata tcctttcagt tactacagat tatggaaggg aacttgcaca
    31501 acaacatgca gagaatgtaa ttttaggtaa gcttggaaaa gaagatatgc taaaatttat
    31561 aaaagataat aatatagtaa aaataataga tgccactcat ccttatgcag ttgaagtatc
    31621 caaaactgct attacttgtg caaaactatt gaatatagat tatataaggt ttgagagaaa
    31681 atctttaatt gaagaaatag attatgataa taaatttata gtagatacaa ttgaagaagc
    31741 ctgtgaaata gcaaataaaa tagggacaaa tatattcata ggaacaggaa gtaaaaattt
    31801 gaacagattt atagaggaaa taagtgataa aaatttagtt gttagagttc ttccaacgag
    31861 tgaagtcata ttaagttgtg aaaaattagg tttaagtgca gataatatag tagctatgaa
    31921 aggtccattt agccagtata taaatgaaga aacatataaa cattacaata tagatttagt
    31981 cataacaaaa gaaagtggaa tagctggtgg gttcttagag aaggtaaatg catgtaaaac
    32041 tttcaatata ccagtaataa ttataaaaag agaatcgatt aactacgaaa atgtatttaa
    32101 tgacataaat aaaattaaag acatacttgt gtaattttta ggaggagaat atatgaagaa
    32161 agccttatta gtagtgagtt ttggtacaag ctatcatgaa acgagagaaa agacaataga
    32221 tatatgtgaa gataaaataa aaaattcgtt aaaaagccat gattttttta gagcttatac
    32281 atcaaatatg ataataaata aaataaaaaa aagggatgga atagaaatag ataatccaat
    32341 acaggcttta gataaaatat atgaacaagg atatgatgaa gtaataatcc aaacacttca
    32401 tattatatgt ggcgaagaat ttaacaaatt aaaagaacaa gtagatggat attcttctaa
    32461 attcaaaaag attgtattag gaagaccttt gcttacacat attgaagatt atcaggaagc
    32521 tgtagaggca attaagcatc aaattcctca tatggaaagt aatgaagcag tagtatttat
    32581 gggacatgga acacttcatg aatcacattc agcgtatcca gcattggaat acatgcttag
    32641 ggataatgga ataaatgctt atgttggtac agttgaaggg tatccagaaa tagaacatgt
    32701 aataagaaga ttaaaagaag gaaatataaa aactgtaaat ttgatgccat ttatgttagt
    32761 tgcaggagac catgctataa atgatatggc aggagatgaa gaagattctt ggaaaacaat
    32821 attagaagaa aatggattta acgtaagagt tcatttaaaa ggactaggag aaaattctta
    32881 tatacaagat aaatttgttc gtcatgctgt aaaatgtgca gagaatgcga agttctatgg
    32941 tataggagca gggccaggag atggttcttt acttacgata aaagcggtta atactttaaa
    33001 gaaactggat attctataca ctccagaatc aaaaaagggt ggagatagtc tagctttatc
    33061 aatagttagt gaatatttac ctgaatcagt ggaaataaag tctaggcatt tcccaatgag
    33121 ttttgatggg aatgaaaaaa gtttagcttg ggacaatgtg gcacaagaaa ttatatcaga
    33181 tgttaatgat ggtaaaaatg taggatttgt tacattagga gaccctatga tttatagtac
    33241 atatgtatat ataatgaaaa gaatattagg agaggtagaa gtcgaaacta taccaggaat
    33301 ttcatcattt tccaatatag catctaatca gaattttcca ttagtcatgg ataaagaagc
    33361 tttagtggta attccttgta ctatggaaga tgataagata gagtatgctt tgcaaaatta
    33421 taattcaata gtattgatga aagtatataa aaattttaaa gaaataataa aaaaattaga
    33481 aaagtatgat ttaatagaac atgctatttt agtaagtaat tcttcccaag aaagcgaaaa
    33541 tgtgtttaca gatttaaaag aagcgcacct tgaagataag atatcttatt tttctactat
    33601 actagtaaat aaagataata aaataaaata ggtgaaagca atatttaaag gagacttaat
    33661 atgaatatag tggttggaac taggggtagt aatctagcgc ttatacaaac agaatgggta
    33721 ataaatgaat taaaaaagaa gtatccagaa attagttttg agattaaaat cataaagaca
    33781 aaaggagatt taattcaaaa tgtttcactg gataaaatag gtgataaagg attgtttgtc
    33841 aaagaaatag agcaacaatt attagatgga aaaatagaca tagcagtaca tagtatgaaa
    33901 gatatgcctt cttatttagc agatggacta aaatttgctc atacgcctaa gagagaagac
    33961 ccaagagatg ttctaatact tagagaagga tataaaaatt tagatgattt acctcatgga
    34021 gcagtaatag gaacaggaag taaaagaagg aagtttcaat tattgaaaca aagacctgat
    34081 ttaaatatag tacaagttag aggtaatgtg gaaactagga tacgaaaaat aaaagatgaa
    34141 aacatgcatg gtattgtgtt ggcagcatca ggaataatta gagctaattt gcaagataaa
    34201 ataagttctt atttacctgt tgatgttgtt attccagcac ctgcccaagg agctttagct
    34261 atagaaatta gaagcaatga tagtgcgata gagggaatag taaattcact gaaggatgag
    34321 aatacagaaa tccaaatatt ggctgaaaga ggttttttag atggtgtaaa tggaagttgt
    34381 catattccaa tggcagctta ttgtgaaata atccaagata aaatacatct tactggctta
    34441 tatggagatt cagaaggaaa aaaagtagta atcaagtcta tagatggaga tatttcttct
    34501 cctagagagt taggtttaaa gttagctaaa cttgtattga aggagtatga gaattatgaa
    34561 gggtaaggtt tacttagtag gggcaggacc aggtgattat aaactcatga ctttaaaagg
    34621 tttagagtgt ataagaaaat cggatgtaat agtatatgat agattagcaa atagtagtta
    34681 tttaagagaa gctaagcctg attgtgaact tatatatgta ggcaaggcat caagtaatca
    34741 tattttgcca caagaagata taaatagaat tatagcagaa aaagctaaag aaggaaaaat
    34801 tgtaactcgt cttaaaggag gagaccctta tgtatttggc agagggggag aagaagcaga
    34861 gactttagta gatgaaggta tagaatttga agtagttcca ggtataactt ctgctatagg
    34921 aggtttatgt tatgcgggta ttcctataac acatagagac catgcttcgt catttcatgt
    34981 aataacaggt catttgaagg gtgatgatag tggagaacta aactggaatg ctcttgcaaa
    35041 taacaaaggt actcttgtgt ttttgatggg aatttcaaac ctagagaaaa taagtgaaaa
    35101 cttaatgaaa gaaggaaaag ataaggatac acctgttgct cttataagtt gggcaactag
    35161 atataatcaa agagttgtta catctacact tgaaaatgtt tatgaaactg ctattaaaga
    35221 agaagtaaaa ccgccaactc ttatagtagt tgggagtgtt gtaggactta gagaaaagtt
    35281 gaacttcttt gaaagtaagc ctttatttgg aaaaagtatt gctgtaacta ggtctagaaa
    35341 tcaaaatagt gtattggtag aaaaaataat ggatttaggt ggaaatccta tagaaatacc
    35401 tactattaag gtagaaaaaa tacaaaataa tattaactta gaaaatgaaa taaaaaatat
    35461 aaataagtat aattatctaa tactaactag taaaaatgcc gtagaaatct tctttgaaaa
    35521 gatatatgaa atgaattttg atttaaggat attatctaat ttaaaggtat gtgctatagg
    35581 ttctgctaca tcaaatgagt tgaaaaaaag aggaattata gcggatatag taccaaaaaa
    35641 atttgtagca gagtcactgt atgaagagtt agcgcctatt ttaaatgatg acgataatat
    35701 acttatacct agagcagaaa atgctagaga ttatctggtt gacaaactta aggctatttg
    35761 tgaagtaaca gaagtacata tatatagaac agtaatagat gaggagaaaa aaggtgagat
    35821 attagatatt ttaaattcta atgacataga ttatataaca tttactagtt cctcaacagt
    35881 aaataatctt gttgaaatta taggaaaaga aaatattaaa atattagaaa aaataaaaac
    35941 actatctata ggtcctataa ctacagagac aataaatagt ttgggaataa aattgtacaa
    36001 agaatctgct acatctacta tagattcgtt agtagattta attataaaaa attaattttg
    36061 gaggcttgaa tatgttaaga agaccaagaa gactaagagc aagtaaagca atcagaaatt
    36121 tggtaagaga aactaaggtg aatgtagaag acttaatata tccactgttt gtagtagagg
    36181 gggaaaatat aaaagaagaa attaaatctt taccagatgt ctatcattta tcaatagata
    36241 tgctagaaga cgaaataaaa gaaataagtg agttgggaat tgaacatata atgttatttg
    36301 gtgttccaga tgaaaaagat gagtgtgctt cacaagcttt taatgataat ggaatagttc
    36361 aaaaggcaat tagaaaggta aaagaaataa attcagatat aaatgtaata acagatgtct
    36421 gtatgtgcca atatacaagt catggacatt gtgggatttt aactgatact ggttatgttg
    36481 ataatgataa atcattggag tatttggcta aaatatctgt aagccatgct aaagcaggag
    36541 ctgatatggt tgctccatct gatatgatgg atggaagaat acaagcaata agagaagctt
    36601 tagatgaaaa tggattagaa aatgttggaa tcatggctta tagtgttaag tatgcttcaa
    36661 attattatgg accttttaga gaggcagctc attcagcacc atcttttgga gatagaaaaa
    36721 catatcaaat ggacccagca aatagcaatg aagctataat agagactgaa cttgatatat
    36781 tggaaggtgc agatatactt atggttaaac ctgcattatc atatttagat attataagaa
    36841 gagttaaaga taattttgac ttacctctag cagcttataa tgtaagtgga gaatactcta
    36901 tgcttaagtt tgctgtgaag gaaggtttat taaatgaaga tgtaattctt gaatcaatta
    36961 catctataaa aagagcagga gcagatataa taataactta ttttgctaaa gacattgcta
    37021 aactgttgaa aaaataaatt ctagcagtat aaagaatatc aaaaagtata ggaattgtaa
    37081 ttaactatta tacagtagtt ttgtttagac atttattgaa gaataataga aactaatttg
    37141 caatttctat actttattta tgtaactaaa taaaatttgg aggtagaaga tgctatatcc
    37201 tattaattta aaattagatg agttagatgt agttataatt ggtggtggag aagttgcata
    37261 taggaaatgt aaaaattttt tagagtttaa caaaaatgtt actatagttt ctaaacaaat
    37321 tttaaataaa ttttatgatt taaaaggaaa tataaaaata ataaaagatg attataaaga
    37381 aatatatata aaagatgctt ctgtaataat tgctgccaca aataatagag agttaaatat
    37441 ggaaatagga ttatattgta aagcaaaaaa taagctagtg aatgtagttg ataatataga
    37501 aatatcgaac tttactgttc catcatatat aaaaagagga gatttactta taagtgtgtc
    37561 tactgggggc aagagtcctt cattatcatc aaagataaaa aaagaaattg aagagagata
    37621 tactgaagat tatgaggagt atttaaatgt attaggagat ataagaaaaa aggttataaa
    37681 aaaatacgaa gataaaagta aaagaaaaga tgtgcttaat atgttggtaa cacttgacct
    37741 agaagaactt aagaagtttg atatataaaa aataaaataa aaaatgttaa aaaggtgttg
    37801 acggattatt tttaag
//
LOCUS       DAEPIN010000037        37295 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.037, whole genome shotgun sequence.
ACCESSION   DAEPIN010000037 DAEPIN010000000
VERSION     DAEPIN010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 37295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37295
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.037"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            16..240
                     /locus_tag="KR250_002049"
     CDS             16..240
                     /locus_tag="KR250_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0067985.1"
                     /translation="MMGSYYTKDFEKEKKNVIKVREVKEDTVRKFFLQGDCEVLVVFE
                     DTGKEILIDDFSPEEDIKKYLGAKFINKKR"
     gene            474..893
                     /locus_tag="KR250_002050"
     CDS             474..893
                     /locus_tag="KR250_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0067986.1"
                     /translation="MQIYFRVKAVGKRKPMLELTPFDVSDNINCLKEAISEIVSKNVL
                     EYNEKIPEKSIINFLTNEEIESQADIGKVGFGSIYNENKQSVEKAIEVALQAFEDGIY
                     KVLINEDVIEKLDSSINLKSGDIFTFIRLTLLAGRMF"
     gene            918..5975
                     /locus_tag="KR250_002051"
     CDS             918..5975
                     /locus_tag="KR250_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HBH0067987.1"
                     /translation="MLERTRVEKNPKIIENFKNNFTKSSSKQYYDLAKSLWDCIENSS
                     YYYREPEDKFLNMIKKYYNCHYYEIKNMDELLSGPLKNALELIFTSEQVEELCQLIKR
                     KMDYTYSGGYYRKGYRSNKLVDYFRGIIRFISNYVDLIYYDFSVIDYLTLPNKVLYYD
                     SIMSDKIALEIDKGNQKVISALEEVIFGENQTALLTRQMIIGIMKSHSDKCYEMLSKL
                     LLAAKQQEGLRQQIVESLDEGTISASIYMLKTILDNNLERFSSVIRALDTWTGLGFSD
                     QKPGIIRKCLELSYKSLTDEKFRIESINSNDNLEIYFALWATATYNIYDSYSLIENLL
                     SCQEKYKKLVALYFLSHTESTYFKHSIASKNIKGDDLEILAWVITNFYVEGSVGYYYS
                     WQNRETNFDLSNTPIDKDFDARKEQFESLKHTLEILNSKTKSFSGSVFPWTWIEISSH
                     EILKCMMTLTAYDMNAQFIDVLMENCHLMSSDLRASLIVNFVNEPKTDRQREFLLECL
                     SDKSVSNRETVLSLLRKLELTDVELDKITNLLSLKTSSIRQTVIKILLEQDDSNLENI
                     ASSLIKSSNSQKCLGGLEIVNLLKDNTARQELYNKLVLLIEDIDKSKKNTSQVQILID
                     NLLGKAKNDYCKENGFGIYNPENKVQIPEQFDFKDDSNSSSKLNKIISNISLSTREDS
                     VSVATKKEAKKILDTDINKIIKVYEKFDELITENADYEYEVERWSGNVDKVLLGYSTY
                     NLTPIKYKVSNDTLDNYPLAELWRETSKKLDLDYKLILDILFYSISTCGYNETEKSWF
                     SSIYSNVLDSKKQNKIYTKLEKLKFNGLTRKLLRVLLNEFDHKEIFNYCLEIYYKLRN
                     LLTIEQCKKQRVEFTKNYYRQNEYCPALNAPILNYWLDKAHSFIYDDISFTKFFYTTF
                     SNYQLLEYKFPFYTSINVFARAHELGLMTEEQVNREMISGYNASNIIRTLTNPRWAGD
                     TLEKYPWLKDNLKTVTNRIIEIESRRGDMPTEVSHLANNIQYFEGINYFVNILVALGK
                     ETFQRGYSYGSNYSKKDVLSSLLKNCYPTKEDDSKKLKSLLKNTDITELRLIESAMYA
                     PQWVNIIEKYLNWKGLKSTIWYFHAHINEGFSAQKESEVALFSPISPRSFIDGAFDVD
                     WFLDSYKTIGEERFNILYKSAKYITSGGNSHRRAQLYADATLGKLDKDMLMKEIETNR
                     NQDKLRSFSLLPMKKGDLKEATFRYEFIQKFAKESKQFGAQRRESESKACTIALENLA
                     RSAGFGDVNRMMWALETEKLNEVAHLFKPKDIDGTSIWIDVDENGKASTKVQKGNKSL
                     KSIPKAIAKHPYVLELKSVQKDLKSQYSRAKQSLEQAMKNEIVFTVKEIANIYKNPVI
                     CPLIKNLVWVCDDNIGYVYNDKSKFFICNIEGETYKLTAKNKLKLAHPVHLFESGKWS
                     EFQHDLFSKQIVQPFKQVFREYYRINSDELSEKTISRRYAGHQIQPKKTVALLRNQLW
                     TVDYEEGLQKVFYKENIVAKMFALADWFSPADIEPPTIETVEFFDRKTYKSLELASIP
                     PILFSEVMRDIDLVVSVAHAGGVDPETSHSTVEMRIAIAKELLLLLKINNVNFEGSHA
                     LIAGNLGEYSVHMGSGIVHKKGMGALNILPIHSQSRGKIFLPFADEDPKTAEIMSKIL
                     LLAEDSKIKDPSILSNL"
     gene            complement(6161..6898)
                     /locus_tag="KR250_002052"
     CDS             complement(6161..6898)
                     /locus_tag="KR250_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0067988.1"
                     /translation="MIKVLICDDDKIVRDDIANIAKESGYVDYVKKVKDGIEAIEFIK
                     NEKIDLLIIDIDMPYKNGIDSAEEILSIDKDIHIVFVTGFADYSLESFKVHPIDFVVK
                     PFKKEKILDSINIAIDHINSHKIANNNFIEDTLFVYKIRKQIHMINFDDIVMFEKNSR
                     AINLHTKDEDIIKFYENFDDLKKRLPPNFFMTHRQYIVNLRFIHKVIPINKSSLEIRF
                     INFQENAILNKSLEKDFLYRFYRVKRF"
     gene            complement(6917..7852)
                     /locus_tag="KR250_002053"
     CDS             complement(6917..7852)
                     /locus_tag="KR250_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBH0067989.1"
                     /translation="MANNFVFPIVILTLFIFSNLIMNKYKFYKELDILKRGILTFFIS
                     CFALILFMEGTKYIQLRLGYSFLLEGVLNIILFLVLIILSFYTGFKVIRLINNNNEQE
                     YKLQKLDSKINHKDNIIIKLRSQKHDYLKHLQVVYSLLNTGLDEDAKEYIMNISNSFK
                     SKNSRYGKVSYLDAIASLKYEECLEKDISFDVYIQDFMDNLEIEPNDMSSVLLNIIDN
                     AIESLSTIKKEKKYIRVHAYEDEFQYIIAIKNNGPKIHNVESIFWNGFSTKEGQDRGY
                     GLHLVKNILDKYDYSILVNSDDFLTEFVILIPKYY"
     gene            8060..10636
                     /locus_tag="KR250_002054"
     CDS             8060..10636
                     /locus_tag="KR250_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBH0067990.1"
                     /translation="MKDNLKIVLRYIKSYKARSLAIVVSIILATSLVVGIGTLSRSAQ
                     QADVDRLKRELGTDHVYFKNIKKNQLKSIKSSKDIKNLGITSYYASTDVNEKLPINIL
                     YANKNYLTSHSKLIKGRFPKASNEVVVEKWILNSMGLKPEINQNLTFKLYQKENPETF
                     KVVGILEDRFIEKKKGQCEIFSNLNESELNDFTTYIEFNEGSDINTNIDNIIKNAMLD
                     EKSIGRNSMLIESTMKNGTLDNSSKYVAIAMSLFAGIVIYSIYVISIYQRVQEYGVLR
                     AIGSTNFRIFKLMLYELFILALIAMPIGICIGMGGAQIFNRSVGNIQFEGNINVTPFV
                     IPDKIILLSIACTILIILIISFFTYLKIRRISPIDAIRKTFGADKNVTKVNYLISKLT
                     LNMSVTKSISAKNIFRNKKGFIIIVLSMSIGGIIVIKDNYKYSFLDVQNKSTQEQTYM
                     NADFILSNVYLNEIGNNTSFKDIKGFNDNQIDKIKNIDGIDKVKTASILNTRIEVNKI
                     NRLDYYEAINSTPYYKLNPLFIKNKTTGKYTLKQKLKGYNDEMIKSLEKYLVSGNINL
                     KKMKKENLAIVYVPEISKTQKYTKTFTPGEGTPAVDIKVGDTIKVKYPKGGMDTGLYE
                     SLKDNYEYLEYEFKVGAIISYPFADNGMYSGDDGVDVITSSEYLKKLTGVDKYNLVYA
                     DLNENANKKKINNLLGKIGSEVPGTTTVDMMVEKENSDKMTSRTMIYSYGIVAIMFII
                     SVLNIINNISYNLTSRTSEFGMLRAIGISERGFKNMILYEGILYGVLSSIITIVSGII
                     IQFKMYYMQGFVSYGLGFSIDYKIYILVILANIIVGILATYIPSRKINKISIVEAINI
                     TE"
     gene            10648..11319
                     /locus_tag="KR250_002055"
     CDS             10648..11319
                     /locus_tag="KR250_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0067991.1"
                     /translation="MPILETINLGKIYGKKETSVHALKNANLKINKGEFVAIIGPSGS
                     GKSTFLHLVGGLERPSNGTIKVDGKDICCLSDKELARYRRQKVGFVFQQYNLIPVLNV
                     KENIELPLKLDNKKIDKEYIDDLINLLGLNERKNHLPNQLSGGQQQRVAIARALSAKP
                     SIILADEPTGNLDSKTTEEVMDLLKSSIKKYNQTLIIITHNENIARKADRIISIIDGE
                     LKLTL"
     gene            11346..11687
                     /locus_tag="KR250_002056"
     CDS             11346..11687
                     /locus_tag="KR250_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0067992.1"
                     /translation="MLLILGNLNLNLDFDYRIIREENDDVDIFIDINYRSLDIDTDGS
                     NLFNSRIQFPFVRALILRLNKNNQCMTIHLLRDIDLFSAFANFEVDYTDSIINIKNQN
                     EKVILNKSIKK"
     gene            complement(12047..14689)
                     /gene="adhE"
                     /locus_tag="KR250_002057"
                     /gene_synonym="adhC"
     CDS             complement(12047..14689)
                     /gene="adhE"
                     /locus_tag="KR250_002057"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HBH0067993.1"
                     /translation="MEKKEKVVEKSNVEVCSPEIVNSVETLRMRLEEIRLAQKEFATF
                     TQEQVDKIFLAASTAANQQRIPLAKMAVEETGMGIVEDKVIKNHFASEYIYNAYKDTK
                     TCGVIEKDEAFGFTRIAEPVGVLSAVIPTTNPTSTAIFKSLIALKTRNGIIFSPHPRA
                     KNCTIEAARVVHDAAVKAGAPKGLIGWVDVPSIELTNVVMAEADLILATGGPGMVKSA
                     YSSGKPAVGVGPGNVPAIIDESADIKMAVSSILVSKSFDNGMICASEQAVIVPEKIYE
                     EVKKEFKYRGAHFLNKEETEKVGKVVIIDGSLNARIVGQPAHVIAKMADVEVPKTARI
                     IIGEVESVELNEPFAHEKLSPVLAMYKSKSFEDAVAKAEKLVADGGYGHTSSLYADSI
                     NHPERVEKFVNAMKTCRVLVNTPSSQGGIGDLYNFKLAPSLTLGCGSWGGNSVSENVG
                     VKHLLNIKTVAERRENMLWFRAPEKVYFKKGCLGVAAREFKDVMDKKKAFIVTDSFLY
                     NNGYTKKLTDLLDEMGIKHTTFFDVAPDPTLACAREGAKAMADFQPDLIIAVGGGSAM
                     DAGKIMWVMYEHPEVDFQDLAMRFMDIRKRVYVFPKMGEKAYFAAIPTSAGTGSEVTP
                     FAVITDQDSGVKYPLADYELMPNMAIIDADMMMEMPPRLTAASGVDALTHALEAYVSM
                     LRTEPADGLALQAGKIIFEYLPRAYKNGKNDKEAREKMAMASTMAGMSFANAFLGICH
                     SLAHKLGAFHHVQHGVANALLINEVIKFNCAEAPNKMGAFSQYRYPDCIQRYAEFASF
                     AGIKGSTDQEKVDNLIKAIDELKAKVGLPKTIKEAGVEESKFLERLDAMVEQAFDDQC
                     TGANPRYPLMSELKEIYLKVYYGK"
     gene            15567..17165
                     /locus_tag="KR250_002058"
     CDS             15567..17165
                     /locus_tag="KR250_002058"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese-dependent inorganic
                     diphosphatase"
                     /protein_id="HBH0067994.1"
                     /translation="MSLLVFGHKNPDTDSICSAISLAHLKNKLGIKATAYALGDIRKE
                     AKYALDYFKVDAPEILDNVRIQVRDLNYDKVVPLTPTSSILEAYNLMDEKNVKTLPVT
                     YDDGTFAGIITMKEIAKNLLHQHFTTIHTSLSNICKNLNGTILVDCGEDIKGRVVTLS
                     FGMETLIETLKEGDIAIVGDRYDSIEYAIDTKVKLLILTNHTEISKDLLALAKINGVS
                     VVSVESDNYKTSNVINQCSFLDSIEATENLITFKEDDYMDEIKEIMLETNFRSYPILD
                     DDNKFLGLVSKGHLLNPSKKNVVLVDHNEYAQSADGIEQANIVEIIDHHKLGGISTDV
                     PMSFRVMPVGCNSTIIYQMYKENNVEIPYEIAGLLLSAILSDTLLFKSPTTTDMDKKA
                     CEELSKIAKVDMEKYAMDMFKCGTSLDEYTIEEIVNMDFKEFNMSGHRVGIGQVFTLD
                     IDSIFAKKDEFLSYINSTDYDKLILSVTDIIKEGSYLIYKAEDSLIANAFGVEGVQGT
                     FAPGVVSRKKQLVPNLTTAIKNFK"
     gene            complement(17402..19483)
                     /gene="bclA1"
                     /locus_tag="KR250_002059"
     CDS             complement(17402..19483)
                     /gene="bclA1"
                     /locus_tag="KR250_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like exosporium glycoprotein BclA1"
                     /protein_id="HBH0067995.1"
                     /translation="MRNIILYLNDDTFISKKYPDKNFSNLDYCLIGSKCSNSFVKEKL
                     ITFFKVRIPDILKDKSILKAELFIHIDSNKNHIFKEKVDIEIKRISEYYNLRTITWND
                     RVSMENIRGYLPIGISDTSNYICLNITGTIKAWAMNKYPNYGLALSLNYPYQILEFTS
                     SRGCNKPYILVTFEDRIIDNCYPKCECLPIRITGPMGPRGATGSTGPTGVTGPTGSTG
                     ATGSIGPTGPTGNTGATGSIGPTGVTGPTGSTGATGSIGPTGVTGPTGNTGVTGSIGP
                     TGATGPTGVTGEIGPTGVTGPTGVTGSIGPTGATGPTGATGVTGEIGPTGATGPTGNT
                     GVTGATGPTGNTGVTGVTGATGPTGATGVTGEIGPTGATGPTGEIGPTGATGPTGATG
                     VTGEIGPTGVTGPTGEIGPTGATGPTGVTGSIGPTGATGVTGEIGPTGPTGVTGSIGP
                     TGATGPTGNTGVTGVTGPTGATGPTGVTGEIGPTGATGPTGVTGEIGPTGATGPTGPT
                     GNTGATGSIGPTGVTGPTGATGATGVTGPTGPTGATGNSSQPVANFLVNAPSPQTLNN
                     GDAITGWQTIIGNSSSITVDANGTFTVQENGVYYISVSVALQPGSSSINQYSFAILFP
                     ILGGKDLAGLTTEPGGGGVLSGYFAGFLFGGTTFTINNFSSTTVGIRNGQSAGTAATL
                     TIFRIADTVMT"
     gene            complement(20161..20685)
                     /locus_tag="KR250_002060"
     CDS             complement(20161..20685)
                     /locus_tag="KR250_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBH0067996.1"
                     /translation="MENKNPIVTIEMENGKEIKIELYPNIAPNTVKNFVSLVNEGYYN
                     GIIFHRVIPGFMIQGGCPNGTGMGGPGHSIKGEFSGNGFTNNLKHERGVISMARTMAP
                     NSAGSQFFIMHKNSPHLDGQYAGFGRVIEGMDTVDEIASVRTDSADKPQTPQIMKSVT
                     VETFGIDYSDVEKN"
     gene            complement(20750..21502)
                     /locus_tag="KR250_002061"
     CDS             complement(20750..21502)
                     /locus_tag="KR250_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HBH0067997.1"
                     /translation="MKLGLCLEGGGAKGAYQAGVVKALYDGGINKFYSISGTSIGAIN
                     GYYLYTGNVDNLEKMWTNIKDIQNGNVKIVNNTVDNSPAIDNLRELDDSNIEEMNFYV
                     NYVEVDNKVVSEKIVDVSKMPRNEAIISISYSGLLPSNPNATLGFKEQFVKDVQEGIY
                     DGFKLDGGLIRSALIEPLIGDNVDKIILISTKYNYELPEDIKKVYDEDKIIVVRPNTQ
                     FAPKDTLNFDDEFCKTIYQEGYEIGKNILDRL"
     gene            complement(21533..22831)
                     /locus_tag="KR250_002062"
     CDS             complement(21533..22831)
                     /locus_tag="KR250_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HBH0067998.1"
                     /translation="MDSINFAKNLIDFIYDSPSSFHAVKSTKEILDKNGFEELVLNQR
                     WNLRVGGKYYVTKNLSAIVAFVVNSEDIERDGFRIIGSHSDSPTFSIKPNAEIEAEKS
                     YLKLNTECYGGAILSTWLDRPLGIAGRVVLKGDSILKPNERLIDFKKPICIIPNLAIH
                     LNRSINDGHSYNKQKDMLPLVGMLNDTLEKDNFLIKQIASNLDVNMDDIIDFDLFLYE
                     FEKGSLIGANDEFISIGRQDNLAMVHASLNALVNTKGQHGVNVMAVFDNEEVGSSTKQ
                     GADSNMLLNILERICISMGKDREGFFESIYSSFMISSDLAHAIHPNIPEKHDPTNKPI
                     MGKGPVIKINASQAYTSDGYSIAIYKNICREANVEYQEFVNRSDERGGSTIGPISSTH
                     IDIPSVDVGSPILAMHSIRELGNVEDHYSIYKTFSKFFEI"
     gene            complement(22848..25112)
                     /locus_tag="KR250_002063"
     CDS             complement(22848..25112)
                     /locus_tag="KR250_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3553 domain-containing protein"
                     /protein_id="HBH0067999.1"
                     /translation="MNLDTLNPAQREAVEKTEGPVLILAGAGSGKTKVLTTRIAYLIE
                     DKGVQAPNILAITFTNKAANEMRERVEQNIGPETKDMWISTFHSCCVRILRKDINKIG
                     YNRSFVIYDSADQVTLVKDCLKELNLSDKVFEPKAVISAISGAKDKLYTPKQFKDINM
                     ADNRMVKIADIYALYQDRLKRNSALDFDDLILKTVELFKANDEVLAYYRSRFRYIMVD
                     EYQDTSKAQYELIKLLAREHQNICVVGDDDQSIYGWRGADIRNILEFEKDYDNVHVVK
                     LEQNYRSTQVILDAANKVISNNIERKRKKLWSEKKEGELIKIQLTGSEIEEADFIADS
                     IAQIARKENRPYKDFAVLYRANAQARPVEDALNRSQIPYNIYGGTKFYERKEIKDLLA
                     YLRVIQNPQDDISIKRIINVPRRGIGLRTIEKIEDRANLKQESIYSVLIDIETNSDIS
                     TKARASISGFVDIIGTLRTIKEVYPVSKLIEKVLDTTGYMDELVEIRNKNEKDLTGKG
                     EEAQDRIDNLREFISIALEFESSNDDTYENKDLETFLTSIALTSESNDEEDNDRVSLM
                     TIHTSKGLEFPVVFLTGMEEGLFPISRAIKSMSDSQIEEERRLCYVGITRAKEELYMS
                     LTEKRTLYGKTNVAIASRFMEELPEECIERLYKVKKELSYSKASYNMLDKYTKKYMST
                     ISKSKVADKVNATIKDSNKETNPDDIKLGSKVHHPKFGVGTVVSIIGTDVTIAFDQQG
                     IKKINKEYTTLNIL"
     gene            complement(25367..26185)
                     /locus_tag="KR250_002064"
     CDS             complement(25367..26185)
                     /locus_tag="KR250_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HBH0068000.1"
                     /translation="MFKINNLTYQYEKNTNALLNINMDFSKGNVIGIIGSNGSGKSTL
                     FMNLMGILKPTSGEILFKEEKLKYDKRSLYNLRKNVGIVFQDPEKQIFYSKVYDDIAF
                     AMRNIGMDEKTIKERINKALVAVNGIDFIDRPVHFLSYGQKKRVAIASVIAMENEIVL
                     LDEPTAGLDPVSTRSIVDIIKGLNKNNIKIVISSHDMNLMYEICDYIYVLDKGILIDE
                     GKAENVFINENNIIQAGLESPWLVKVHRNMNLPLFKKEEDLYKYWKERELNTNK"
     gene            complement(26178..26876)
                     /gene="cbiQ"
                     /locus_tag="KR250_002065"
     CDS             complement(26178..26876)
                     /gene="cbiQ"
                     /locus_tag="KR250_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HBH0068001.1"
                     /translation="MLIIDKYAYTNRLSKVNPNKKVAIGVIFLIASMVIQNIFILSGI
                     MILMSILVVCVAGIDLKNYLKLLRIPMYFLFLSIGITLVNISFNKADLLYSFEFFSFN
                     VGISKASIDMSIHVLFRAMSCLTCVYFCILTTPFNQLIFFFKKLHLPDTFVELSMLIY
                     RFIFIFLEEFSEIYKSQELRFGYINLKTSYKSLGILGSMLYKRLMTRYDDMCISLDIK
                     LYDGKFHIVGDNDV"
     gene            complement(26879..27157)
                     /locus_tag="KR250_002066"
     CDS             complement(26879..27157)
                     /locus_tag="KR250_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     substrate-binding protein"
                     /protein_id="HBH0068002.1"
                     /translation="MSAKTKTNILLLLLVVALIIFPLLVNSGAEYGGADGQAESEITK
                     INPNYKPWFSSPYEPPSGEIESLLFSAQAALGAGVIGYILGLQKGKRS"
     gene            complement(27147..27899)
                     /locus_tag="KR250_002067"
     CDS             complement(27147..27899)
                     /locus_tag="KR250_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HBH0068003.1"
                     /translation="MKQNIKLGVIAALMLIILTPVTSNAMHIMEGYLPVKWSIAWGVI
                     FIPFFLFGLKSIGKIVKQDPKKKVLLALCGAFVFVLSALKIPSVTGSCSHPTGVGLGA
                     IMFGPSVMFVLGTIVLIFQALLLAHGGITTLGANAFSMAIIGPIVSFLIFKALKKKDG
                     NNAIPVFLAAAIGDLATYTITSIQLALAFPDPSGGVMASAIKFLGIFFMTQIPIAIAE
                     GILTVIVYNLITENGEKSILENNDKGVKANEC"
     regulatory      complement(28081..28273)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(28405..30177)
                     /locus_tag="KR250_002068"
     CDS             complement(28405..30177)
                     /locus_tag="KR250_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase activation-like protein"
                     /protein_id="HBH0068004.1"
                     /translation="MKWIYDDYEFENRANNLSWTIAGDYNESIDISEKDYSSKEIGLY
                     FAIIAGARRKYVDWDIVKKYLTNRIRKGYNKDIILGLIQVILNTIVEEKVIKDRPGIE
                     DIRRNAYKDIVSRFTKIHNDNIMDKIKYTFVLYSMDKHPALDSMTRRIVNDIRNIDTN
                     QDIMEILKALDVIYLTYFEYILNGEENSLIDENNVESVEVNFDTFADFMYEELYSDEE
                     ENIIESDIDSISSSMLVEGVGDFDDSKCSNSADRVIYVDEDTANKIYSKIEHYYGKTY
                     LSKGEIKKIETQNCRNVHEGCRIHFTDGVLRSECNNVFQLKYVTRQKENNLGKFREHA
                     RMYRQQIKRLKESLSRILIEENAKSRVYSDCGTILANRAWRVGRSNNNKIFYKDIENE
                     KGKYVIDILLDASGSQSRNQGNVAIQAYIIANALTEVGIPNRVMGFSSFLDYTILKRF
                     KDYDDSIKNSENIFEYFCAGNNRDGLAIKSICSGLINRDEENKILIVLSDGRPNDVKI
                     GKSSERTLRGEKAYRGIVGLRDTANEVRKARKQNILVLGVFTGKESELEAERLIYGND
                     FIYSRDITRFSDVVSMYLKKIIRN"
     gene            complement(30191..31120)
                     /locus_tag="KR250_002069"
     CDS             complement(30191..31120)
                     /locus_tag="KR250_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBH0068005.1"
                     /translation="MDIIDNLRVQGVDEKLIEDVLYFRNYYGLEKDLEYRVTKSKTYF
                     YGKDILSMCIAAILEEENILLSGPKATGKNLLADNLGEIFNRPQWNTSFHINTDSSTL
                     IGTDTFIDNEVKLRRGSVYECAINGGFGVFDEINMAKNDAIVVVHSALDYRRIIDVPG
                     YERVNLHPATRFIGTMNYEYAGTKELNEALVSRFMVIDIPPIEEDKLMMILKNEFSDA
                     DEEKLIHFAGIFLDLQLKSQNGEISSKAIDLRGLMASLKTIRRGLKPTLAINMGLTGK
                     TFDVYEKEMVGDVIKTRIPNKWESIDVFPISHI"
     gene            31349..31537
                     /locus_tag="KR250_002070"
     CDS             31349..31537
                     /locus_tag="KR250_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH0068006.1"
                     /translation="MEYMSCPEVAKKWGISERRVQKLCEESRIPGVSKLGYMWLIPKN
                     AEKPVDGRTKRRKEVQYE"
     gene            31530..32231
                     /locus_tag="KR250_002071"
     CDS             31530..32231
                     /locus_tag="KR250_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0068007.1"
                     /translation="MNSVLIIDDDKELCALMKKCVEQENLSAITAHGGAEGLHLVDKN
                     RHTLSLVILDIMMPDIDGFQVLKKIRETSNIPVIMLTAKNDEDDKVSGLRLGADDYLT
                     KPFSINELMARVNSLIRRYTMLNPAFITDTDFIIINGMIIDKTNRIVSVNNIPVQLTS
                     KEFDLLAFLASNKGRVFTKKQIYTQVWEEEYAFDDSNIMSFISKLRKKIEPDPNHPFY
                     ILTVRGVGYRFNKEA"
     gene            32233..33156
                     /locus_tag="KR250_002072"
     CDS             32233..33156
                     /locus_tag="KR250_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBH0068008.1"
                     /translation="MNTSVYLLLILALSLIIIAYLVSVLLRVRIQLTLIKDALEDIKN
                     GNLNRRILTDENDITKQICYDINEIAINSQFQLIQQKQSEQAYKQLMTSLSHDVKTPL
                     ASLVGYLEAVESKIVVGEEKDEYIHVASNKAHYLKNFVENLFEWVKLDSKEQVFHFDI
                     FDLNELSRNIISDWIPVLESSHFEYEFDIPEIEYFLRIDANAYTRIINNLLQNIITHS
                     SGNKMTLRIFENKEQAQIIITDNGKGISSDNLPHIFERLYQCDHSRASKGNGLGLAIA
                     KELINAHKGNITANSTPGMGTEFTILLPKAL"
     gene            33313..34236
                     /locus_tag="KR250_002073"
     CDS             33313..34236
                     /locus_tag="KR250_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019639192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0068009.1"
                     /translation="MNDFVIETKQLTKIYGNQTVVDKVNLHVKKGRIYGLLGRNGAGK
                     TTIMKMILGLTSITYGEVDVFGENIKGREKQVYPRIGAIIETPGFYPNLTGTENLEIF
                     AKLRGTAAPNSVKRALEIVGLPYKNKKLFSKYSLGMKQRLGIANAILHDPELLVLDEP
                     TNGLDPIGIAEMRKFIRDLSVEHGKTILISSHILSEISLLADDIGIIDHGVLLEESSM
                     SELEKKNSKYILLQVSDIPKTSLILERQFNTKDYSVQDDYTLHLYNTNLDMGEINKAL
                     VMQNVTVISSQLCNDTLEDYFKKITGGEGIA"
     gene            34229..34966
                     /locus_tag="KR250_002074"
     CDS             34229..34966
                     /locus_tag="KR250_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0068010.1"
                     /translation="MLKLIQTEFLKLRRRKLIWLMLLSALIMPFFALLYFNYFGKTGV
                     EPIKFYKWSAFGYTLWIILPVVLGIFCTMLIHEENQYDMLKQLWIVPISKMGYFFSKF
                     FVVLIYSICFMLITAVASVLFSVLSGYVVFSWASILYLLKKCLEIGVITAFVMLPILA
                     IATSQKGYILPICITLVYAFSGFVIMTINMYLHPLSSMGVIIMRNKDIPGLIFSQEIN
                     VPLAFLCICIWSIVSVLFANIALGRRK"
     gene            34977..35750
                     /locus_tag="KR250_002075"
     CDS             34977..35750
                     /locus_tag="KR250_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0068011.1"
                     /translation="MWKLLWAECQKIRRSKIVWITVFASIMIAVIVFAGGQDVYNDPD
                     LHYGLKSVYDGSRYIDNAGWYMDAVQPWSTFFVLPAVIALLGSYIICREEEEDTIKSL
                     RLIPINETNLTIAKMIITFIFSILLYLLLFTITFLTESVLHFSDLSTKLVLSCMKEYF
                     LDGIGVFFAISPIIALVSRMKKGYWLALVFTEVYSIAGLFAGMSNVLQTFFPIAAIFN
                     ISGYYITTGEKIMGSIMSLLLCACLSAFILKGLKHNEKN"
     gene            complement(36291..36899)
                     /locus_tag="KR250_002076"
     CDS             complement(36291..36899)
                     /locus_tag="KR250_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBH0068012.1"
                     /translation="MSVINSFILQYGLISVFVLIMIEYACFPLPSEVVLPLCGAIAAR
                     NHFGFLTILILSIIAGILGSIFCYTVGNWGGKSIINKIIEICPKAKKGIFASQDYFNK
                     YSSISVCVCRLIPLCRTYISFIAGIAGQNIITFVISSIVGITIWNTSLISIGYIFSES
                     WVRIMSHYNDYKFLVLIIPISIIFIGFVIKSFIHKKHHKIRL"
     gene            complement(37077..37193)
                     /gene="rrf"
                     /locus_tag="KR250_002077"
     rRNA            complement(37077..37193)
                     /gene="rrf"
                     /locus_tag="KR250_002077"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT        12665 a         5950 c         5040 g        13640 t
ORIGIN      
        1 acaaaaataa ttaatatgat gggaagttat tacacaaaag actttgaaaa agaaaaaaag
       61 aatgtaataa aagttagaga agttaaagaa gatacagtta gaaaattttt cttacaaggt
      121 gactgtgaag ttcttgtagt atttgaagac actgggaaag aaatacttat tgatgacttt
      181 tctcctgaag aagatatcaa aaaatattta ggagcaaaat ttataaataa aaaaagatag
      241 ttatcaaaaa taattctaaa attaaaattt aataaatgta cagttataat tctatataat
      301 tatagttaaa agttatcttt tattgctttt ttagacttta agatagcttt ttttctattt
      361 tttgtaaaat aattccataa ttcacattaa taattcacaa aatgatataa tataacaaat
      421 agataaattt ttaaatttct aaatacataa ttaatatttt gcgaggtgat tttatgcaaa
      481 tttattttag agttaaggct gttggcaaac gcaaacctat gctagaactt accccttttg
      541 atgtatctga caacatcaat tgcctaaaag aagctatctc tgaaattgta tctaaaaatg
      601 ttcttgaata taatgagaaa atcccagaaa aatcaataat aaacttctta actaatgaag
      661 aaattgaatc tcaagctgat attggaaaag ttggatttgg ctctatttac aatgagaata
      721 aacaatctgt agaaaaggca atagaggtag cgttacaggc atttgaagat ggaatctaca
      781 aagtgcttat aaatgaagat gttattgaaa aactcgattc ctctataaat ctgaaaagtg
      841 gagatatatt tacttttata agacttacct tgcttgctgg aagaatgttt taaaataaat
      901 aatttggagg tacataaatg ttagaacgta ctagagtaga aaaaaatcct aaaataattg
      961 aaaattttaa aaataatttt actaaatctt cttcaaaaca gtactacgac ttagcaaagt
     1021 ctctttggga ctgcatagaa aatagttcat actattatag agaacctgaa gacaaattct
     1081 taaatatgat aaagaaatac tacaattgcc actactacga aattaaaaat atggacgagc
     1141 tcctttctgg acctttaaaa aatgcattag aattaatatt tacttctgaa caagtagaag
     1201 aactttgcca attaattaaa agaaagatgg attatacata ttcaggagga tattatcgta
     1261 aagggtatcg ctcaaataaa cttgtggatt attttagagg tattataagg tttatttcaa
     1321 actatgttga tttaatttat tatgattttt cagttataga ttatcttact cttccaaata
     1381 aggtcttgta ctatgactct ataatgagtg ataaaatagc actagaaata gacaaaggaa
     1441 atcaaaaagt aataagtgct ttagaagaag taatatttgg agaaaatcaa actgctctac
     1501 ttacaagaca aatgataatt ggtatcatga agagtcatag tgataaatgc tatgaaatgc
     1561 tttctaaact tcttttggca gccaaacaac aagaaggact tagacagcaa attgtagaga
     1621 gtttagatga gggtacaatt tctgcatcaa tatacatgtt aaaaactatt ttggacaaca
     1681 atttagagcg atttagttct gttataagag ccttggatac atggacagga cttggttttt
     1741 cagaccaaaa acctggaata atacgtaaat gtttagaact ttcatataaa tcattgactg
     1801 atgaaaagtt ccgtatagaa tcaattaata gcaatgataa tcttgaaatt tattttgcct
     1861 tatgggctac tgcaacttac aacatatatg actcttactc tcttattgaa aacttacttt
     1921 cttgtcagga aaaatataaa aaacttgtag ctctttactt tttatcgcat acagaaagta
     1981 catattttaa acattctata gcatctaaaa acattaaggg tgatgattta gagatactag
     2041 catgggtaat tacaaatttc tatgtagaag gttctgttgg ctactactat agttggcaaa
     2101 acagagaaac taattttgac ttgtcaaaca ctcctattga caaagacttt gatgctagaa
     2161 aagaacaatt tgaaagttta aaacatactt tagaaatttt aaattcaaag acaaaatcat
     2221 tttcaggaag tgtatttcca tggacttgga ttgaaatttc ttctcatgaa atacttaaat
     2281 gtatgatgac tttaacagca tacgatatga atgctcaatt tatagatgtg cttatggaaa
     2341 attgtcatct tatgagttca gatttaagag cttcacttat agtcaatttt gtaaatgaac
     2401 caaagacaga tagacaacgt gaatttttat tggaatgttt aagtgataag agtgtttcca
     2461 atagagaaac ggtattgagt ctacttagaa agcttgagct aacagatgta gaattagata
     2521 agataaccaa tctattgtct ttaaagacaa gctctattag acaaacagta ataaaaattt
     2581 tacttgaaca agatgattca aatttggaaa atattgcttc ttcactgata aaatcttcaa
     2641 attctcaaaa atgccttgga ggtcttgaaa ttgtaaactt attaaaagac aatactgcta
     2701 gacaagaact atataataag cttgttttac ttatagaaga tattgataaa tctaaaaaga
     2761 atacatctca agttcaaata ctaattgata atttgttagg taaagcaaaa aatgattact
     2821 gcaaagaaaa tggttttgga atatataacc ctgaaaacaa agttcaaatt ccagaacaat
     2881 ttgattttaa agatgattct aatagctcat caaagcttaa taaaattatc tcaaatataa
     2941 gtttatctac tcgtgaggat tctgtttctg tagcaacaaa aaaagaagca aaaaaaattc
     3001 tcgatacgga tataaataaa ataataaaag tttatgaaaa atttgatgag cttattactg
     3061 aaaatgcaga ttatgaatat gaagttgaaa gatggtcagg taatgtagac aaagtacttt
     3121 taggttattc tacttataac ttgactccta ttaaatataa agtttctaat gatactcttg
     3181 ataattatcc attagcagag ctttggagag aaactagtaa aaagcttgac ttagattaca
     3241 aattaatact tgatatttta ttttatagta taagtacatg tggatataat gaaacagaaa
     3301 aaagttggtt ttcatctata tattcaaatg tccttgactc taaaaaacaa aataaaatat
     3361 atacaaaact tgaaaaactt aaatttaatg gacttacaag aaaactttta agagttttac
     3421 taaatgagtt tgaccataaa gaaatattta actattgttt agaaatatac tataaactta
     3481 gaaacttatt gactatagaa caatgtaaaa aacaaagagt tgaatttact aaaaactatt
     3541 acagacaaaa tgaatactgc cctgctttaa atgcccctat tttaaactat tggcttgata
     3601 aagcacattc gtttatttat gatgatattt cttttactaa gtttttctac actacgtttt
     3661 caaattatca gttgctagaa tataagtttc ctttctatac tagcataaat gtgtttgctc
     3721 gtgcacatga attaggatta atgactgaag agcaagttaa tagagaaatg ataagtggat
     3781 acaacgcatc aaatataata agaacattga caaatcctag atgggctggc gacacattag
     3841 aaaagtaccc ttggcttaaa gataatttaa aaactgttac aaaccgaatt atagaaattg
     3901 aatcacgtag aggtgatatg ccaacagagg tgtctcatct agctaataat atacaatact
     3961 ttgaaggtat aaattatttt gtaaatattt tggtcgctct tggtaaagaa acgttccaga
     4021 gaggatattc atatggtagc aattattcaa aaaaagatgt tttaagttct ctacttaaaa
     4081 attgctatcc tactaaagaa gatgactcaa aaaaattaaa aagtttactt aaaaataccg
     4141 acattactga gttaagattg atagaatctg ctatgtatgc ccctcaatgg gtaaacataa
     4201 ttgaaaaata tttaaactgg aagggtctga aaagtacaat ttggtacttc catgcacata
     4261 taaatgaagg cttctctgca caaaaagaat cagaggttgc tctattctcc cctatttcac
     4321 cacgtagttt cattgatggt gcatttgatg tagattggtt tttagattca tataaaacta
     4381 ttggggaaga aagattcaat atactttaca agtcagctaa atatataact agtggtggta
     4441 actctcatag acgtgcacaa ctctatgctg atgctacact tggtaagctt gataaagaca
     4501 tgttgatgaa agaaattgaa acaaatagaa atcaagataa attgagaagt ttctctttac
     4561 ttcctatgaa aaaaggagac ctaaaagaag caactttccg ttatgaattt attcaaaagt
     4621 ttgctaaaga aagcaagcaa tttggtgcac aacgtcgtga aagtgaatca aaagcttgta
     4681 ctatagcgct agaaaatcta gctagaagtg ctggatttgg agatgtaaat cgtatgatgt
     4741 gggctcttga gactgaaaaa ctaaatgaag ttgctcatct atttaaacca aaagatattg
     4801 atggtacttc aatttggata gatgttgatg aaaacggaaa agcatctact aaagttcaaa
     4861 aaggcaacaa gtctttaaag agtataccaa aagctattgc taaacatcca tatgtacttg
     4921 aacttaaatc tgtccaaaaa gatttaaaga gtcagtattc tcgtgcaaaa cagagtttag
     4981 aacaagctat gaaaaatgaa atagtattta cagttaaaga aattgccaat atctataaaa
     5041 atccagttat ctgccctttg attaaaaatc tagtatgggt atgtgatgat aatattggat
     5101 atgtttataa tgataagtct aagttcttta tttgcaatat agaaggtgaa acttataagt
     5161 tgacggcaaa aaataaatta aaacttgcac atccagttca tttgtttgaa tctggtaagt
     5221 ggagtgagtt ccaacatgat ttatttagta agcaaattgt tcaaccattt aagcaagtat
     5281 tccgtgagta ttatagaata aacagtgatg aattatctga aaaaactata tctcgcagat
     5341 atgctggtca ccaaattcag ccaaagaaaa ctgtagcact tcttagaaat cagctttgga
     5401 ctgtcgatta tgaagaggga ttacagaaag tattttataa agaaaatata gtggcaaaga
     5461 tgtttgcact tgcagattgg ttttcaccag cagatataga gcctcctact attgaaacag
     5521 tagaattttt tgatagaaaa acttataaaa gtttagaatt agcttctata cctccaattc
     5581 tattctcaga agttatgcgt gatatagacc ttgttgtaag tgtcgctcat gctggtggtg
     5641 ttgaccctga aactagccat tctactgtag agatgcgtat agcaattgct aaagaacttt
     5701 tattattatt aaaaattaac aatgtcaatt ttgaaggctc tcatgctctt atagcaggta
     5761 atttaggtga gtattctgtg catatgggtt ctggtattgt acataaaaaa gggatgggtg
     5821 ctttgaatat acttcctata cattctcaaa gtagaggtaa gatattcctt ccattcgctg
     5881 atgaagaccc taaaactgct gagattatgt ctaagatact attacttgct gaagatagta
     5941 agattaaaga ccctagtatc ttaagtaatt tataagtata aaaactatcc ctaaaatata
     6001 ttggactttc ttaaaaaaaa ttagaaagtc caatatatac tatactgtaa tttatataga
     6061 ttaaatcata attacaaata atttttatat aaacttctct tatacattat ctttatggta
     6121 cttattatat cacacaaaaa gtatttttct tctatcttca ctagaatctt ttaaccctat
     6181 aaaatctata taaaaaatct ttttctagag atttgtttaa tatggcattt tcttgaaaat
     6241 ttataaacct tatctctaat gaagatttat ttataggtat aaccttatgt ataaatctta
     6301 gatttactat gtattgtcta tgggtcataa agaaattagg aggtaggcgt ttttttaaat
     6361 catcaaagtt ctcataaaac tttattatat cttcatcttt agtgtgtaga ttaattgctc
     6421 ttgaattttt ctcaaacata actatatcat caaaattaat catatgtatc tgcttcctta
     6481 ttttatatac aaaaagggtg tcttctataa aattattatt agcaatctta tgggagttaa
     6541 tatggtctat tgctatgtta attgaatcaa gtattttttc ttttttaaat ggtttaacta
     6601 caaaatctat aggatgcact ttaaagcttt caagactata atcagcgaaa cccgtcacaa
     6661 atactatatg tatatcctta tctatagaaa gaatttcttc agcagaatca atcccatttt
     6721 tatatggcat atcaatgtca ataatcaata aatctatttt ctcatttttt ataaattcaa
     6781 ttgcctctat accatcctta acttttttaa cataatctac atatccagac tctttagcta
     6841 tatttgctat atcatctctt acaatcttat catcatcaca aatcaaaact tttatcatag
     6901 acttttctcc tcctaactaa taatattttg ggattaagat tacaaattca gttaaaaaat
     6961 catcactatt tacaagtata ctataatcat acttatctaa tatattttta actaaatgta
     7021 gcccatatcc cctatcttgt ccttctttag tactaaatcc attccaaaat atactttcaa
     7081 cattatgtat tttaggacca ttatttttta tagctataat atactgaaat tcatcttcat
     7141 atgcatgtac tcttatatac ttcttttctt tttttatagt agacaaagac tctattgcat
     7201 tatctattat gtttaatagt actgaactca tatcattagg ttcaatctct aaattatcca
     7261 taaaatcttg aatgtagaca tcaaaagata tatccttttc aagacattcc tcgtatttta
     7321 atgaagctat agcatctaaa taagatactt ttccatacct tgaatttttt gatttaaaac
     7381 tatttgatat attcattata tattctttgg catcttcatc tagcccagta tttaatagag
     7441 aatacaccac ttgcaaatgt ttcaaatagt catgtttttg acttcttaat ttgataatta
     7501 tattatcctt atgatttatt ttactatcta gtttttgtag tttatattcc tgttcattat
     7561 tattatttat caatcttata actttaaagc ctgtataaaa acttaaaata attaaaacca
     7621 aaaaaagtat aatatttaat actccttcaa gtaaaaaact atatcctaac cttaattgta
     7681 tatatttagt gccctccata aataaaatca atgcaaaaca cgatataaaa aatgttaata
     7741 tgcctctttt taatatgtct aattctttgt aaaatttata cttattcatt ataagattgc
     7801 taaatataaa caaagttagt attactatag gaaatacaaa attgttagcc atgatttccc
     7861 tcccctctac aatttatata gggattatat cattttaaaa aaattatgta aaaagttaaa
     7921 atttacatat agattaatta aacttgtaaa aatacagatg aatttgtaca attaactact
     7981 taaaacttga aaatacttac tttgctatat attttagata aatatataat tgatttagtt
     8041 aagaaaggaa gtggtaaata tgaaagataa tttgaaaata gtactaaggt atataaaaag
     8101 ttataaagct aggtctttag caattgttgt gagtataatt cttgctacat cactcgttgt
     8161 tggtattggt accctctcaa gaagtgctca gcaagcagat gtagatagat tgaaaagaga
     8221 gcttggaact gaccatgtat actttaaaaa tataaagaaa aatcaattaa agtccataaa
     8281 atctagcaaa gatattaaaa atctgggtat aacatcttac tatgcgtcta cagatgttaa
     8341 tgaaaaatta cctataaaca tactatatgc aaataaaaat tatttgacta gtcattctaa
     8401 acttataaaa ggtcgatttc caaaagctag caatgaggtg gtagtcgaga aatggatttt
     8461 aaatagtatg ggcttaaaac cagagataaa tcaaaacctt acttttaaat tatatcaaaa
     8521 agaaaatcca gaaactttta aagttgttgg aatccttgaa gatagattta tagagaaaaa
     8581 aaagggacaa tgtgaaattt tttctaatct caatgaatca gaactgaatg attttacaac
     8641 ttatattgaa tttaatgaag gcagtgatat aaatacaaac atagacaata taataaaaaa
     8701 tgctatgtta gatgagaaaa gtataggaag aaactcaatg cttatagaat ctactatgaa
     8761 aaacggtact ttagataatt caagtaaata cgttgcaatt gctatgagtc tatttgctgg
     8821 tattgtaata tatagtatct atgttatatc aatataccaa agagtacagg aatatggcgt
     8881 cttaagagct attggttcca ccaactttag gattttcaaa cttatgcttt atgaactttt
     8941 catacttgct ctaatagcaa tgcctatagg tatatgtata ggtatgggag gtgcacaaat
     9001 atttaatagg tctgtaggaa atattcaatt tgaaggaaat ataaatgtta caccttttgt
     9061 tattccagat aaaataattc tcttatcaat tgcatgtaca atactaataa tactaattat
     9121 aagtttcttt acatacttaa aaataagaag aatttctcct attgatgcta tcagaaaaac
     9181 ttttggagct gataaaaatg taaccaaagt taattattta atatcaaaat taactttaaa
     9241 tatgtcagta acaaaatcta tatctgcgaa aaacatcttt agaaataaaa aggggttcat
     9301 tataattgta ttatctatga gtataggagg tataatcgtc ataaaagaca attataaata
     9361 ttctttttta gatgtgcaaa acaaatctac tcaagaacaa acatatatga atgcagattt
     9421 tattctatca aatgtttatc tcaatgaaat tggtaacaat acttctttta aagatataaa
     9481 agggtttaat gataatcaga tagataaaat aaaaaatata gatggaattg acaaagtcaa
     9541 aactgctagt atcttaaaca caagaataga ggtaaataaa ataaatagat tagattacta
     9601 cgaagctata aattcaactc cttattataa gctaaatcct ctgttcataa aaaataagac
     9661 tactggcaaa tatacactaa agcaaaagct aaaaggttat aatgatgaga tgataaagtc
     9721 attagaaaaa tacttagtaa gtggaaatat aaacttgaaa aaaatgaaaa aagaaaattt
     9781 agctattgta tatgtaccag aaatttctaa aacacagaaa tacacaaaga cttttactcc
     9841 tggtgaagga acaccagctg tagatataaa agtaggtgat actataaaag tcaaatatcc
     9901 aaagggtggg atggatacag ggctttatga aagtctaaaa gataattatg aatacttaga
     9961 atatgaattt aaagttggag ctattataag ttatcctttt gcagataatg gtatgtactc
    10021 tggagatgat ggtgtagatg taataacgag tagcgaatac ctaaaaaaat taactggtgt
    10081 agataagtat aacttagttt atgcagacct taatgaaaat gcaaacaaga aaaaaataaa
    10141 taacctttta ggtaaaattg gaagtgaagt tcctggaact acaacagtag atatgatggt
    10201 agaaaaagaa aattctgata agatgacatc tagaactatg atttatagtt atggtatagt
    10261 agcaataatg ttcataatca gtgtacttaa cataataaac aacataagtt ataacttaac
    10321 ttcaagaact agtgaatttg gaatgttaag agctattggt atctctgaga gaggctttaa
    10381 aaatatgata ctttatgaag gaatattata tggtgtactc tcaagtataa ttactatcgt
    10441 gtcaggaatt ataatacaat ttaaaatgta ttatatgcag ggctttgtat catatggatt
    10501 gggattttca atagattaca aaatatatat cctagttatt ttagcaaata taattgttgg
    10561 tattctagct acatatattc catcaagaaa gataaataaa attagtatag ttgaagctat
    10621 aaacattaca gaataggagg gataattatg cctatattgg aaactattaa tttaggtaag
    10681 atttatggga aaaaggaaac atctgtacat gcactaaaga atgcaaattt aaaaataaat
    10741 aaaggcgaat ttgttgctat aattggacca agtggaagtg ggaaaagtac atttctacat
    10801 ttagtaggtg gtcttgaaag accaagtaat ggtactataa aggtagatgg taaagatata
    10861 tgttgtcttt cagataaaga attagcaaga tatagaagac aaaaggtagg atttgtcttt
    10921 caacagtata acttaattcc tgtattaaat gtaaaggaaa atatagaatt accactaaag
    10981 ttagacaata aaaaaataga taaagaatat attgatgacc ttataaattt attgggtctt
    11041 aatgaaagga aaaatcatct tcccaaccaa ttatctggtg gtcaacagca aagagttgct
    11101 attgcacgtg ctttatctgc aaaaccaagt ataatcctag cagatgaacc aacaggaaat
    11161 ttggacagta aaactacaga agaagtcatg gatttactaa aaagttctat aaaaaaatat
    11221 aaccaaaccc ttataataat aacacataat gaaaatattg ctagaaaagc agatagaatt
    11281 atatctataa tagatggaga gcttaaatta acactctaaa actatttata gaaaagagga
    11341 tttaaatgtt attgatttta ggtaatttaa atttaaactt ggattttgac tacagaataa
    11401 taagagaaga aaatgatgat gtagatatat ttatagatat taattatcgt agcttagata
    11461 tagatactga tggttctaac ttatttaatt ctagaataca atttccattt gtaagagcat
    11521 tgattctaag gctaaataag aacaatcaat gtatgacaat tcatttgtta agagatatag
    11581 atttattctc tgcttttgcc aactttgaag tggattatac tgactctata ataaatataa
    11641 aaaatcaaaa tgagaaagta attttaaata aatctatcaa aaaatagtaa gggggcctta
    11701 ataggcccca atttttttct tttctaaact tattatatca tgataaataa attagtcaat
    11761 agaattttct gaatattttt ataatttttt cataaagttt tttatatatt atgctttaaa
    11821 attatatgta ataaaaaaat tttccgacta attttaaagt taattgatta aatacacata
    11881 ttcgaaatta ccattaaatt actgactttt tatcatttcc atttagaatt taagttataa
    11941 tctattattt atagttactt ctacgtgtct ctaataaaac tctatttata ttcaaatcta
    12001 ataatttata tacaaaccca atagaaccta tgttgcacaa tagatattat ttaccataat
    12061 aaacttttag atatatctct tttaattcac tcatcaatgg gtatctagga ttagcacctg
    12121 tacattggtc atcaaaagct tgttcaacca tagcatccaa tctttctaag aatttagatt
    12181 cttctactcc agcctctttt atagtcttag gaagacctac ttttgccttt agttcatcta
    12241 ttgcttttat aagattatct actttttctt ggtctgtact tcctttaata cctgcaaaag
    12301 aagcaaattc agcatatctt tgtatgcaat caggatatct gtattgtgag aatgcaccca
    12361 ttttatttgg agcttctgca caattgaact tgattacttc atttattaat aatgcatttg
    12421 caacaccgtg ttgaacatgg tggaatgcac ctaatttatg tgctaaagag tgacatattc
    12481 ctaagaaggc atttgcaaaa gacattcctg ccatagtaga tgccattgcc attttttctc
    12541 ttgcttcttt atcattcttt ccatttttat atgcacgagg taaatattca aatattattt
    12601 tacctgcttg tagtgctaga ccgtcagctg gttcagttct taacatagat acatatgctt
    12661 ctaatgcatg tgttaaagca tcaacacctg atgctgctgt taatcttggt ggcatctcca
    12721 tcatcatatc agcatcaatt atagccatgt ttggcattaa ttcataatct gctaatgggt
    12781 actttacacc tgaatcttgg tcagttataa ctgcaaatgg agttacttct gaaccagttc
    12841 ctgctgaagt tgggatagca gcaaaatacg ctttttcacc catcttagga aatacatata
    12901 ctctttttct tatatccata aatctcatag ctaagtcttg gaaatctact tctggatgct
    12961 catacattac ccacattatc ttaccagcat ccattgcact tcctccacct acagctatta
    13021 ttaaatctgg ttggaaatca gccatagctt tagctccttc tcttgcacaa gctaatgttg
    13081 ggtctggagc tacatcaaag aatgtagtat gcttaattcc catctcatct aataaatcag
    13141 ttaatttctt agtatatcca ttattatata ggaaagaatc tgttactata aatgctttct
    13201 ttttatccat aacatcctta aattctcttg ctgctacacc taaacatcct tttttgaaat
    13261 aaaccttttc tggtgctctg aaccaaagca tattctctct cctttcagct actgtcttga
    13321 tattaagcaa atgttttact ccaacgtttt cagatactga gtttccaccc caagaaccac
    13381 aaccaagagt aagagacggt gccaatttaa agttatataa atcacctata ccaccttgtg
    13441 atgatggagt attaactaat actctacaag tcttcatagc atttacaaac ttttctactc
    13501 tctctggatg atttatagag tctgcatata atgaagatgt atgaccatag cctccatctg
    13561 caactaattt ttctgcttta gcaactgcat cttcaaaact tttagacttg tacattgcaa
    13621 gtacaggaga aagtttttca tgtgcaaaag gttcatttaa ttcaacagat tctacttcac
    13681 caattattat tctagctgtt tttggaactt ctacatccgc cattttggct attacatgtg
    13741 ctggttgtcc aactattctg gcatttaatg aaccatcaat tattactact tttcctactt
    13801 tttcagtttc ttctttattt aggaaatgtg ctccacgata tttaaattct tttttaactt
    13861 cttcatatat tttttctggt actattacag cttgctcgga agcacatatc ataccattat
    13921 cgaatgattt tgatactaag attgaactta cagccatttt tatatctgca ctctcatcta
    13981 ttattgcagg tacatttcca ggcccaactc cgacagctgg tttacctgaa gagtatgcag
    14041 atttaaccat tccaggtcca ccagtagcta atataaggtc tgcctctgcc atgacaacat
    14101 tagttaattc aatcgaagga acatctaccc aacctataag tccttttggt gctcctgcct
    14161 taactgctgc atcatgtaca actctagctg cttctatagt acagttttta gctcttggat
    14221 gaggagagaa aattatacca tttctagttt ttagtgctat tagggatttg aatattgcag
    14281 ttgatgtagg attagttgtt ggtataactg ctgataaaac acctactggc tcagcaattc
    14341 ttgtaaaacc aaatgcttca tctttttcta tgacaccaca agttttagta tctttataag
    14401 cattatagat atattctgag gcaaaatggt tttttataac cttatcttca acaataccca
    14461 taccagtctc ttccactgcc atttttgcta gaggtattct ttgttgatta gctgcagttg
    14521 aagcagctaa gaaaatctta tctacctgtt cttgcgtaaa tgttgcaaat tctttttgtg
    14581 ccaatctaat ttcttctaat ctcattctta atgtttcaac actgttgaca atttcaggac
    14641 tacatacttc aacatttgat ttttctacta ccttttcttt tttctccata ctaaaaacct
    14701 ccctaaaata attatgcatc tctaattttt attaatctta aatgcatcat ctatattttt
    14761 aacttatact taaaataatt ttatagattt catctctata ccttagattt tatttatttt
    14821 actctcttag gtcaagaagt ttttttctga atattttgtc aatttatttt ttaacaaata
    14881 tattattttt tttactttta ctaaaatttt ttatctaaat gagatatatt tcatttgaat
    14941 aatttacttc gactaaataa aatactaaaa tgataattta tatctttcat tttttttact
    15001 tattaaattt tatatgttat ttgcatgtta tttgtataag tagtaaataa aatttttgtc
    15061 gtatttttat aaaataacac ttatttttga cttgtatgca atttttcttt ttataacaac
    15121 tgtatagttt taaaaaatat ataaactaaa attattctaa tctctataag ttatttttaa
    15181 gtcaatatgt aataattatg cttttcatat agataaataa tttttatatt aagaccgtta
    15241 attttttaat gataatatat atacaaaatt ttttacagta taaaatattt ataaattttt
    15301 aatttattaa catattaatt aattttttcc caggtagatt taatcaattt attttaaata
    15361 aaagtaaatt taattcgata tttgattggg taaaagtata tataataata atttttatat
    15421 taaatttgta gtatttatgt aagaaataaa ttttatataa tattttttca ctaaattttt
    15481 ttctttttac aacaaatact cactaccttt tgatataata tttttatcaa ttattattat
    15541 attcaagttt tgaaaggagt attaaaatga gtttattagt tttcggacat aaaaatccag
    15601 atacagattc aatatgttct gcaatttcat tagcacattt aaagaacaaa ctaggaataa
    15661 aagcaacagc ttatgcttta ggagatatca gaaaagaagc taaatatgcc ttggattact
    15721 ttaaggtaga tgcacctgaa attttagata atgttagaat acaagttaga gatttaaatt
    15781 atgataaagt agtaccttta acaccaactt catctatatt agaggcttat aaccttatgg
    15841 atgaaaaaaa tgttaaaaca ctacctgtaa cttatgatga tggaactttt gctggtatca
    15901 ttactatgaa agaaatagct aaaaatctac ttcatcaaca ctttacaact atacacacat
    15961 cactatcaaa catttgtaaa aaccttaatg gtactattct tgtggactgt ggtgaagata
    16021 taaaaggtag agtagtcact ttatcatttg gaatggagac tctaatagag actttaaaag
    16081 aaggagacat agctattgtt ggagatagat atgatagtat tgaatatgct attgatacaa
    16141 aagttaaact tttaatactt actaatcata cagagatttc aaaagatttg ctagctcttg
    16201 ctaagataaa tggagtgtct gttgtttctg ttgaaagtga caattataaa acttctaatg
    16261 taataaatca atgtagtttc ttagactcta tagaagctac agaaaattta attacattca
    16321 aagaagatga ttatatggat gaaataaaag agattatgct agaaacaaac ttcagatctt
    16381 atccaatatt agatgatgat aataagtttt taggtttagt ttcaaaaggt catctattaa
    16441 acccatctaa aaagaatgtt gttttagttg accataacga atatgctcaa agtgctgatg
    16501 gaattgaaca agcaaacata gttgaaataa ttgaccatca taaacttggc ggtatttcta
    16561 ctgatgttcc tatgtcattt agagttatgc ctgttggatg caatagtact ataatttatc
    16621 aaatgtacaa agaaaataat gttgagatac cttatgaaat agctgggctt cttttatctg
    16681 ctatattatc agatacatta ttatttaagt ctccaacaac aactgatatg gataagaaag
    16741 cttgtgaaga attaagtaaa atagcaaaag tagacatgga aaaatacgct atggatatgt
    16801 ttaaatgcgg tacttcttta gatgaatata ctatagaaga aatagtaaat atggacttta
    16861 aagaatttaa tatgagtgga catagagttg gtattggaca agtatttacc ttagacatag
    16921 actctatatt tgctaaaaaa gatgagtttt tatcttatat aaattcgact gattatgata
    16981 aattaattct ttcagttaca gatataatca aagaaggttc ttatttaatc tataaagcag
    17041 aagatagctt aattgctaac gcatttggtg ttgaaggtgt tcaaggcaca tttgcaccag
    17101 gtgttgtatc aagaaaaaaa caattagtcc ctaatttaac tactgcaatc aaaaatttta
    17161 aataatataa aatttttaaa taacgaaaac tgtctcaaat ctatatttga gacagttttt
    17221 tattatatat atatattgat aataaagtta aataaaaatc cataaattta aaatttttat
    17281 gaatatattt tattattatt gttattttat tcattctaaa ataggtaaaa ctcaatatat
    17341 aaaaatactt ttgtcattta aataaaacct aaatagtttt taagattatt ttagacacgt
    17401 tttaagtcat aacagtatca gctattctaa atatcgtcaa agtagccgca gttcctgctg
    17461 attgcccatt tcgtatccct actgttgtag atgaaaagtt atttattgta aaagtagtcc
    17521 caccaaataa aaaaccagca aaatatccag aaagtactcc tccgcctcct ggctcagtag
    17581 taagccctgc caaatctttt cctcctaaaa ttgggaatag gatagcgaaa gaatattgat
    17641 ttatacttga tgaacctggt tgtaatgcta ctgaaactga tatataatac acaccatttt
    17701 cttgtactgt aaacgtacca tttgcatcta ctgttatact tgaactattt cctattattg
    17761 tttgccaacc tgttatagca tctccattat ttagtgtttg tggagatggt gcatttacga
    17821 ggaagttagc aactggctga gaggaattgc ctgttgctcc tgttggacct gttgggcctg
    17881 ttactcctgt cgctcctgtt gctcctgttg gacctgttac tcccgttggc cctatacttc
    17941 ctgtcgctcc tgtatttcct gttgggcctg ttggtcctgt tgctcccgtt ggtcctattt
    18001 ctcctgttac tcctgttggg cctgttgctc ccgttggtcc tatttctcct gtcactcctg
    18061 ttgggcctgt tgctcccgtt gggcctgtta ctcctgtcac tcctgtattt cctgtcggtc
    18121 ctgttgctcc cgttggtcct atacttcctg tcactcctgt tgggcctgtt ggtcctattt
    18181 ctcctgtcac tcctgtcgct cctgttggtc ctatacttcc tgtcactcct gttggtcctg
    18241 ttgctcctgt tggtcctatt tctcctgttg ggcctgttac tcctgttggc cctatttctc
    18301 ctgtcactcc tgtcgctcct gttgggcctg ttgctcccgt tggtcctatt tctcctgttg
    18361 ggcctgttgc tcctgttggc cctatttctc ctgtcactcc tgtcgctcct gttggacctg
    18421 ttgctcctgt tactcctgtt actcctgtat ttcctgttgg acctgttgct cctgttactc
    18481 ctgtatttcc tgttggacct gttgctcccg ttggtcctat ttctcctgtc actcctgtcg
    18541 ctcctgttgg acctgttgct cctgttggtc ctatacttcc tgtcactcct gttgggcctg
    18601 ttactcccgt tgggcctatt tctcctgtca ctcctgttgg gcctgttgct cccgttgggc
    18661 ctatacttcc tgtcactccc gtatttcctg tcggacctgt tactcctgtt ggtcctatac
    18721 ttcctgtcgc tcctgtactt ccggttgggc ctgttactcc cgttggccct atacttcctg
    18781 ttgctcctgt atttccggtt gggcctgttg gtcctatgct tcctgtcgct cctgtacttc
    18841 cggttgggcc tgttactcct gttgggcctg tacttcctgt cgctcctctt ggtcccattg
    18901 gacctgtaat tctaattgga agacactcac atttaggata acaattatct ataattctat
    18961 cttcaaatgt tacaagtata tacggtttgt tacaacctct actagatgta aattcaagaa
    19021 tctgataagg gtaatttaaa gataaagcta acccataatt aggatattta ttcattgccc
    19081 atgcttttat agttcccgta atatttaaac aaatatagtt ggatgtatca cttatcccaa
    19141 ttggtaaata tcccctgata ttttccatag acactctatc attccatgtt atagttcgta
    19201 aattataata ttcacttatt cttttaattt caatatctac tttttcttta aaaatatgat
    19261 tcttatttga atcaatatga ataaataact ctgcttttaa tatacttttg tcttttaata
    19321 tatctggtat tctcacttta aaaaaagtaa tcaacttttc ttttacaaaa ctatttgaac
    19381 atttacttcc tattaagcaa taatctaaat tactaaagtt tttatctgga tattttttag
    19441 atataaaagt atcatcattt aaataaagta taatatttct catttataat ccctccattt
    19501 tgggtctata tcatactatg tttaaaatac atgcttagtt acaatacaat taaaatttat
    19561 agtattaaat ttttatataa taatgctgat ttctacaaaa aaagaacatg tcattagaaa
    19621 tttctaatag catgttcttt ttcattatta tatattacac tttaaattat tccttgactg
    19681 gcttagctgg tgtaaactaa accagtcttt ctaaaatatt aagcactttt ttcttattat
    19741 gttttttagg atttattgtc tatattattg taaaccaaag ttataaaatt gtaaatagaa
    19801 tatattacca taacaatatt tttttataag taaaaattat aaaaaacttt atttttgtta
    19861 aatggaataa ataaaataag tttaaaaaat actgaaataa gtgttttcca catattttat
    19921 atatttattt aatacattat tatttaaaaa taaatatata aacaatcata ataaaatttt
    19981 tttaataaaa ctttattttt tagttaaaat catacaatat atagatttcc acaatacaaa
    20041 taaattaaaa agtgaaatgt atttgtctaa aaacataata acatttcact ttatcctcat
    20101 ttttaagtaa tatatatttt taatttttat ttataactaa atctagtttt tatataaatt
    20161 ttagtttttt tctacatctg agtaatctat tccaaaagtt tctacagtaa cagatttcat
    20221 aatttgtgga gtttgtggct tatcagctga atctgttcta actgatgcta tttcatcaac
    20281 tgtatccata ccttctatca ctctaccaaa acctgcatac tgaccatcta agtgtggtga
    20341 gtttttatgc attatgaaaa attgagaacc agctgaatta ggtgccatag ttctagccat
    20401 tgatatgact cctctttcat gttttaaatt gttagtaaat ccattaccac taaattcacc
    20461 ttttattgag tgacctggac cacccattcc tgtaccattt gggcatcctc cttgtatcat
    20521 aaatcctggt attactctat gaaatattat tccattgtaa tatccttcat ttactaatga
    20581 tacaaagttt ttaactgtat ttggagctat atttggatat aactcaattt tgatttcttt
    20641 tccattttcc atttctatag ttactatagg atttttattt tccataattt attaccttcc
    20701 ttttcattag ttaaattaca taattacatt tattatatta tactctaaat taaagtctat
    20761 caagtatatt ttttcctatt tcatatcctt cttgataaat agttttacaa aactcatcat
    20821 caaagttaag cgtgtcttta ggagcaaatt gagtattagg tctaactact attattttat
    20881 cttcatcata tactttttta atatcttctg gcaactcata attatatttt gtggatatta
    20941 aaataatttt atccacatta tccccaatta atggttcaat taatgcactc ctaattaaac
    21001 ctccatctaa tttgaaacca tcataaattc cttcttgcac atctttcaca aattgttctt
    21061 taaatccaag agttgcgttt ggatttgatg gtaacaaccc actgtatgaa atgctgataa
    21121 tggcttcatt acgaggcatt tttgatacat caacaatctt ttctgatact actttattat
    21181 caacttcaac ataattaaca taaaagttca tttcttctat attactatca tctagttccc
    21241 ttaggttgtc tatagcagga gaattatcca cagtattatt tactattttt acatttccat
    21301 tctggatatc ttttatattt gtccacattt tctccaagtt atcaacattc cctgtgtaca
    21361 aataatatcc atttattgct cctattgatg tccctgaaat cgaataaaat ttatttattc
    21421 ctccatcata caaggcttta actactcctg cttgataagc cccttttgct cctccacctt
    21481 ctaaacataa tcctaatttc atagtctcat ctccaaattg tttatcttat atttatattt
    21541 caaaaaactt actaaatgtt ttatatatac tatagtggtc ctcaacattt cctaattctc
    21601 ttatagaatg cattgctaaa attggacttc ccacatccac agatggtata tctatatgag
    21661 tagaagatat tggacctata gtacttcctc ctctttcatc tgacctatta acaaattctt
    21721 gatactcaac attagcttct ctacatatat ttttataaat agctatagaa tatccatcac
    21781 tagtatacgc ctgactagca tttatcttta taaccggacc ttttcccatt attggtttat
    21841 tagttgggtc atgcttttct ggaatgtttg gatgaatagc atgagctaaa tctgatgata
    21901 tcataaaaga tgaataaata gactcaaaga atccctctct atccttaccc attgatatac
    21961 aaattctctc caatatattt aacaacatgt ttgaatctgc accttgttta gtagagctac
    22021 caacttcttc attgtcaaat acagccatta catttacacc atgttgacct ttagtattaa
    22081 caagagcatt caaactagca tgtaccattg caagattatc ttgtctccca atagatataa
    22141 attcatcatt agcccctatt aaacttcctt tttcaaattc atataaaaat aaatcaaaat
    22201 ctataatatc gtccatattt acatctaagt tagatgctat ttgctttata agaaaattat
    22261 ctttttctaa tgtatcattt aacatcccaa ctaaaggtaa catatctttt tgcttattat
    22321 atgaatgacc atcatttata cttctattta aatgtattgc tagatttggt attatacaaa
    22381 ttggcttttt aaagtctata agcctttcat ttggttttaa tatactatca ccttttaaaa
    22441 ctactctacc agctatccca agaggtctat ccaaccatgt acttaatatt gctcctccat
    22501 agcattctgt atttaacttc aaataagatt tttcagcttc tatttctgca tttggtttaa
    22561 tactaaatgt aggagaatct gaatgagagc ctattattct aaatccatct ctttctatat
    22621 cttctgaatt tactacaaaa gctactatag ctgataaatt cttagtaaca taatactttc
    22681 caccaactct tagattccat ctttgattca aaactaattc ttcaaatcca tttttatcta
    22741 agatttcctt agtactcttg actgcatgaa aagacgacgg actatcatat atgaagtcta
    22801 ttaagttttt agcaaagttt atactatcca ttcctcattt acctccatta taaaatattt
    22861 agtgttgtat attctttatt tattttttta attccctgtt ggtcaaaagc tatagtaaca
    22921 tctgtaccaa ttatagaaac cactgtccca accccaaatt taggatgatg taccttagaa
    22981 ccaagtttta tatcatctgg attagtttct ttattagaat cttttattgt agcattaact
    23041 ttatctgcaa ctttagattt gcttatagta ctcatatact ttttagtata tttatctaac
    23101 atattataac ttgctttaga atagcttagt tcttttttta ctttgtacaa tctttctata
    23161 cattcttctg gcagttcttc cataaatctt gaagctattg caacatttgt tttaccatat
    23221 aatgttctct tctctgtcaa tgacatatat agctcttctt tagctctagt tataccaaca
    23281 taacaaagtc ttctctcttc ttctatttgt gaatcactca tagattttat agctcttgat
    23341 ataggaaata acccttcttc catacctgtt aaaaacacaa cagggaactc taaaccttta
    23401 gatgtatgaa ttgtcataag cgaaacccta tcattgtctt cttcatcatt tgattcagat
    23461 gtaagtgcta tacttgttag gaatgtctct aagtctttat tttcataagt atcatcatta
    23521 ctactttcaa actccaaagc tatagaaata aattctctca aattatcaat tctgtcttga
    23581 gcttcttcac ctttgcctgt taaatctttt tcatttttat ttcttatctc tacaagctcg
    23641 tccatatatc ctgtagtgtc caagactttt tctataagtt tgctaacagg ataaacttct
    23701 tttatagttc taagagtccc tataatatca acaaatccac ttatacttgc ccttgcctta
    23761 gtagatatat ctgaatttgt ttctatatca attaatactg agtatatact ttcctgtttt
    23821 aagttagctc tatcttcaat tttttctatg gttctaagcc ctatacctct tctaggaaca
    23881 tttataatcc tttttataga tatatcatct tgtggatttt gaataactct taaatacgcc
    23941 aacaaatcct ttatttcttt tctctcatag aattttgtac caccatatat attgtatgga
    24001 atttgacttc tatttaaagc gtcctctaca ggtcttgcct gtgcatttgc tctataaagt
    24061 actgcaaaat ccttataagg tctattttct tttctagcta tttgtgcaat tgaatcagca
    24121 atgaaatcag cctcttctat ttcactacca gttaattgta tctttataag ttctccttct
    24181 tttttttcac tccaaagctt ttttcttttt ctttctatat tattagatat aactttatta
    24241 gctgcatcta atatcacttg tgtagaccta taattttgtt ccaactttac aacatgtaca
    24301 ttgtcataat ctttctcaaa ttcaagtatg tttcttatat ctgcacctct ccatccataa
    24361 atactttggt catcatctcc aacaacacaa atattttgat gttctcttgc taataatttt
    24421 atcaattcat attgagcttt acttgtatct tggtactcat ctaccattat gtatctaaat
    24481 ctacttctgt aatatgctaa cacttcatca ttagccttga aaagttctac agttttaagt
    24541 attaaatcat caaagtcaag tgcactattt ctttttaaac ggtcttgata tagcgcataa
    24601 atatctgcaa tcttaaccat tctattatct gccatattga tatctttaaa ctgctttgga
    24661 gtataaagtt tatcttttgc tcctgaaatt gcacttataa ctgcttttgg ttcaaacact
    24721 ttatcactta aatttaattc ttttagacag tctttaacca atgtaacttg gtctgctgaa
    24781 tcataaatta caaaacttct attgtaacca atcttattta tatcttttct tagtatacga
    24841 acacagcaag aatggaatgt acttatccac atatccttag tttcaggacc tatattttgt
    24901 tcaactctct ctctcatttc atttgcagct ttgtttgtaa atgttatagc taatatattt
    24961 ggtgcttgta cacctttatc ttctataaga tatgctattc ttgttgttaa taccttagtt
    25021 ttacctgaac cagcaccagc cagtataagt actggacctt cagtcttttc tactgcttct
    25081 ctttgagcag gatttaatgt atctaaattc atatttctcc tcctgttaac agccataaat
    25141 atattttaat ctaaaatatg aattacttca aattataatt atgtattata ttaatagatt
    25201 atttccaata tattacaatt aaataacatt tttccatata atttatttca ttgtttaata
    25261 aaaaaaataa ccttatgatt tggggaatca taaggcacct ttaaggggtc taggttaagg
    25321 gttattatat ataaatctat ttttttaaaa taggtttagg gcttaattat ttatttgtat
    25381 taagttctct ttccttccaa tacttataca aatcttcttc tttcttaaat aagggtaaat
    25441 tcatatttct atgtactttt actaaccatg gggattctag gccagcttgt ataatattat
    25501 tttcatttat aaacacattt tctgcttttc cttcatcaat caaaatcccc ttatccaata
    25561 catatatgta atcacatatt tcatacatta aattcatatc atgactggat attactattt
    25621 ttatattatt tttatttaat ccctttatta tatctacaat agacctagta cttactgggt
    25681 ctaaaccagc tgtaggttca tcaagtaata caatttcatt ttccattgct ataactgatg
    25741 ctattgccac tctttttttc tgtccatagc taagaaaatg tactggcctg tctataaaat
    25801 ctattccatt aacagctact agagctttgt ttattctctc tttaattgtt ttttcatcca
    25861 taccaatgtt tctcattgca aaggctatat catcatatac ttttgaataa aaaatttgtt
    25921 tttctgggtc ttggaacaca atacctacat tttttctaag gttgtataaa cttctcttat
    25981 catattttaa cttttcttct ttaaaaagaa tttctccaga tgtaggcttt aatataccca
    26041 ttaaattcat aaataatgtt gattttcctg aaccatttga acctattata cctataacat
    26101 ttccctttga aaaatccata ttaatgttta atagtgcatt ggtatttttt tcatattgat
    26161 acgttaaatt atttatttta aacatcatta tcccctacta tgtgaaattt accatcatac
    26221 aattttatgt ctaatgatat acacatatca tcataccttg tcataagtct tttgtacagc
    26281 atacttccaa gaatacctaa ggatttatat gaagttttta aatttatata tccaaatctt
    26341 agctcttgtg acttgtatat ttctgaaaat tcttctaaaa atatgaatat aaacctatat
    26401 attaacattg ataactcgac aaaagtgtca ggtaaatgaa gttttttaaa aaagaatatt
    26461 aattgattaa aaggtgttgt aagtatacaa aaatacacac aagtcaaaca agacatcgct
    26521 ctaaataaca catgaataga catgtcaata gacgcctttg atattcctac attaaaacta
    26581 aaaaattcaa aactatataa taaatctgct ttattaaaag atatatttac aagagttatt
    26641 cctatactaa gaaataaaaa gtacattggt attctaagta attttaaata attttttaaa
    26701 tctattccag ccacacaaac aaccaaaata ctcattaaaa tcataattcc acttaaaata
    26761 aatatatttt gtattaccat agaagcaatc agaaaaatta ctcctatggc tacttttttg
    26821 tttggattta ctttactcaa tctatttgta tatgcatatt tatcaattat aagcatgatt
    26881 aacttctctt tcctttttga agacctaata tgtatcctat tacaccagcc cctaaagctg
    26941 cttgtgcaga gaataataaa ctttctattt ctccacttgg tggctcatat ggactgctaa
    27001 accatggttt gtaatttgga tttatcttag taatttcact ttcagcttga ccatctgctc
    27061 ctccatattc agcacctgag ttgacaagta atgggaatat aataagagct actactaata
    27121 aaagtaataa tatgttagtt tttgttttag cactcattag ctttaactcc tttatcatta
    27181 ttttctagta tgcttttttc tccgttttct gtaattaaat tatatacaat tacagtaagt
    27241 ataccttctg ctattgctat tggtatttga gtcataaaga aaattcctaa aaatttaatt
    27301 gctgaagcca ttaccccacc tgatgggtct gggaaagcca gtgctaattg tattgaagtt
    27361 attgtatatg tggctaagtc tcctattgct gctgcaagaa atactggaat agcattattt
    27421 ccatcttttt tctttaaagc cttaaatatt aggaatgata cgattggccc tattatcgcc
    27481 attgaaaaag cattagctcc taaagtagtt attcctccat gtgctaataa caatgcttgg
    27541 aatattaaaa ctatagttcc aagaacaaac attacgcttg gtccaaacat tattgctcca
    27601 agacctacac cagtaggatg tgaacaactt cctgttactg aaggtatttt tagtgctgat
    27661 aatacaaaca caaatgctcc acataaagct aataatactt tctttttagg gtcttgcttt
    27721 actattttac ctattgattt taatccgaat aagaaaaatg gtataaatat aactccccat
    27781 gctatagacc attttactgg taagtaacct tccattatat gcattgcatt tgatgtaact
    27841 ggagttaaaa tgattaacat aagtgcagct ataaccccta atttaatatt ctgtttcatc
    27901 ttagtatccc tctctccctt tctaataatt aaaacataat aatacatgtc tgatttattt
    27961 aataaaataa aaaagcctaa ctcgatggtt aggcttctaa ttttccacaa tctaaataca
    28021 aacacataaa acaaacatat ctgtataatt attataagaa actttctcca tccccgaaga
    28081 aattacttta atattttagg caggtctcct gacttagctt catcctactc ctcttccttc
    28141 ccatgagata taaaaaatat catcatagtg gctaacaaaa gtttcgtctg catcacagta
    28201 gcgggggctg taaaggaatt taaccttttt ccctattaat cttgcttaaa gaacctataa
    28261 tattatatat ttaattttta aattcttctt aaattatttt ctatataaat cattgtatac
    28321 cttttctgtg aaaatttcta cattttttat aaattttatt catttttata tttttatgta
    28381 tataaattct tttttgtaag tttattaatt tctaattatc ttctttaaat acattgaaac
    28441 tacatcagaa aatctagtta tatctcttga atatataaaa tcatttccat atattaatct
    28501 ttcagcttca agttctgatt cttttcctgt aaatactcct aatactaata tattttgctt
    28561 tctagctttt ctcacttcat tggctgtatc tctcaaacca actatacccc tataagcctt
    28621 ttctcctctt aatgttctct cactactttt tcctatcttt acatcatttg gtcttccatc
    28681 actgagaact atcaggattt tattttcttc atctctattt attagaccac tacatataga
    28741 ttttattgcc aaaccatctc tattattacc agcacaaaaa tattcaaata tattttcact
    28801 attttttata ctatcatcat aatctttaaa tctttttaat attgtatagt ctaaaaaact
    28861 agaaaatccc atcactctat ttggtatgcc aacttctgtt agagcatttg caattatgta
    28921 agcttgaatt gccacatttc cctgatttct actttgagag ccacttgcat ccaataatat
    28981 atcaataacg tatttccctt tttcattttc tatatcttta taaaatattt tattattatt
    29041 acttctacca actctccaag ctctattggc taatatagta ccacaatcag aatatactct
    29101 acttttagca ttttcttcta ttaaaattct tgatagactt tcttttaatc tctttatttg
    29161 ctgtctatac attctagcat gttctctaaa ttttcctaaa ttattttctt tttgtcttgt
    29221 tacatatttt agttgaaata cattattaca ctcacttcta agaactccat ctgtaaagtg
    29281 tattctacaa ccttcatgaa catttctaca attttgtgtc tcaatttttt taatttcacc
    29341 tttactcaaa taagtttttc cataataatg ttctattttt gaatatatct tatttgcagt
    29401 gtcttcatca acatatataa ctcggtctgc tgaattgcta catttagaat catcaaaatc
    29461 ccctacacct tctacaagca tggatgaaga tattgaatct atatcacttt caataatatt
    29521 ttcttcttca tcactataaa gttcttcata catgaaatca gcaaatgtat caaaatttac
    29581 ttctacactt tctacattat tttcatcaat aagactattt tcttctccat ttaatatgta
    29641 ttcaaaataa gttaaatata taacatctaa tgcttttaaa atctccataa tatcttgatt
    29701 ggtatctata tttcttatat cattaactat tcttctagtc atactatcta atgctgggtg
    29761 cttgtccata ctataaagta caaatgtgta tttaatttta tccataatat tatcattgtg
    29821 tattttggta aaacgtgaaa caatatcttt ataagcattt cttcttatgt cttctatccc
    29881 tggcctatct ttaatgacct tttcttcaac tattgtattt aatataactt gaattagacc
    29941 cagtataata tctttgttgt atccttttct tattctattt gtaagatatt ttttaactat
    30001 atcccaatct acatactttc ttctagcacc tgcaattatt gcaaaatata gacctatttc
    30061 ttttgaagag taatcttttt ctgatatatc tatactttca ttgtaatcac cagctattgt
    30121 ccatgataag ttattagctc tattttcaaa ttcataatca tcatatatcc atttcataat
    30181 ctcacctata ttaaatatga cttattggaa acacatctat actctcccat ttattaggta
    30241 ttctagtttt aattacatct ccaaccattt ctttctcata tacatcaaaa gtctttcctg
    30301 taagtcccat attaattgca agagttggtt ttaatcctct tcttatagtc ttaagagatg
    30361 ccataagacc acgtaaatca atagctttac tagatatctc tccattttga gacttcaatt
    30421 gtaaatctaa aaatattcct gcaaagtgta ttagtttttc ttcatcagca tctgaaaatt
    30481 cattttttaa aatcatcatt aacttatctt cttctatagg aggaatatct attaccataa
    30541 atctagatac cagtgcttca ttaagttctt ttgttcctgc atattcataa ttcatagtcc
    30601 caataaatct tgttgctgga tgtagattga ctctctcata cccaggtaca tcaattattc
    30661 ttctatagtc taaagctgaa tgtaccacaa ctatagcatc attttttgcc atattaattt
    30721 catcaaacac cccaaaacct ccattaatag cacactcata aactgagcct cttctaagtt
    30781 taacttcatt gtctataaat gtatctgtac ctataagtgt tgaactgtct gtatttatat
    30841 gaaatgatgt attccactga ggtctattaa atatttcccc tagattatca gcaagtaagt
    30901 tttttcccgt tgcctttggt cccgataata atatattttc ttcttctaaa atagctgcta
    30961 tacacataga caagatatct ttaccataaa aataagtttt agactttgtt actctgtatt
    31021 ctaaatcttt ttctaatcca taataatttc taaaatacag tacatcttca ataagttttt
    31081 catctacccc ttgcaccctt agattatcta ttatatccat atttttaccc cttttatata
    31141 aatagtattt gtttcatttc ttatcaccat tttagcataa aaggatataa gctaaaattg
    31201 attttttaat ctcccccatt ttaagtaaaa ccatgcacca ttttatcact acaaaattaa
    31261 gagtactact tactgatatt tcttgatttt attcgtctat acgtatataa tatatactga
    31321 taaattatga ggaaaggatg attgaaatat ggaatatatg tcttgcccag aagtagcaaa
    31381 aaaatggggt atttctgaaa gaagagtaca aaaactatgt gaagaaagtc gcataccagg
    31441 agtttcaaaa ctcgggtata tgtggctgat accaaagaac gcagaaaagc ctgttgatgg
    31501 tagaacaaaa agaagaaagg aagtacaata tgaatagcgt tttgattata gatgatgaca
    31561 aagaactttg tgctcttatg aaaaaatgtg ttgagcaaga aaatttatct gctattactg
    31621 cacatggtgg tgcagaaggt ctacatttag tcgataaaaa tagacacaca ctctctcttg
    31681 taattttgga cataatgatg ccagatatag acggttttca agttctgaaa aaaattagag
    31741 aaacaagtaa tattccagta attatgctta ctgctaaaaa tgacgaggat gataaagttt
    31801 ccggactacg tttaggtgct gacgattacc taacaaaacc gttcagcata aacgaactta
    31861 tggctcgtgt caattctctt attcgtcggt atactatgct aaaccctgct tttataacag
    31921 acacagattt cataataata aatggcatga ttattgacaa gacaaaccga attgtttctg
    31981 tcaataatat tcctgttcaa cttacaagca aagaatttga tttacttgct tttctggctt
    32041 ctaataaggg acgggtgttt accaaaaaac agatttatac acaagtatgg gaagaagaat
    32101 atgcttttga cgatagtaac atcatgtctt tcatcagtaa attgagaaaa aagattgaac
    32161 cagaccccaa ccatccattt tatattttga cagttcgtgg tgtcggttat cgttttaata
    32221 aggaggctta acatgaatac aagtgtctat ctgcttttga tattggctct tagcctgatt
    32281 attatagcgt accttgtttc tgtcctgcta cgtgtccgaa tacaacttac acttataaaa
    32341 gatgctttag aggatataaa aaatggaaac cttaatcgac gtattttaac agatgaaaac
    32401 gacattacaa aacaaatttg ctatgatatt aacgaaatag caatcaatag ccaatttcaa
    32461 cttattcagc aaaaacaatc agaacaagcc tacaaacagc taatgacaag tctctcccat
    32521 gatgtaaaaa ctccacttgc ttctttggtc ggttatttgg aagcagtaga aagtaaaatt
    32581 gttgtaggag aggaaaaaga cgagtatatc catgttgcgt ctaataaggc tcattactta
    32641 aagaattttg tagaaaatct atttgaatgg gtaaaattgg attctaagga acaagttttt
    32701 cattttgata tttttgactt gaatgaatta tcccgtaata ttatatctga ttggatacct
    32761 gttttagaaa gcagtcattt tgaatatgaa tttgatatac cagaaataga gtacttctta
    32821 cgtatagacg caaatgcata tacccgtatc attaataacc tattacaaaa tattattacc
    32881 catagtagtg gaaacaaaat gacattgaga atttttgaaa acaaagagca agctcaaatt
    32941 ataataacag ataatgggaa aggtatatct tcagacaatc tcccacatat ttttgaaagg
    33001 ttgtaccaat gcgaccattc acgagcttca aagggaaatg gtttaggctt ggcgattgca
    33061 aaggaactta ttaacgctca taagggaaat attaccgcca acagcactcc cggtatggga
    33121 acagaattta caatattgct tcctaaagcc ctgtaaaagt acgaggcttt cttactctaa
    33181 aacaagataa aagcaagatt atggcaaggt tttggcaagg ttcatgtttt ataattctaa
    33241 ttgtagttga ttttgacgtt gtatcaacta tcttttgtag aaacaaattt attaaaagtg
    33301 aggaaattat atatgaatga ttttgtgatt gaaacaaagc aacttacaaa aatttatggg
    33361 aatcaaacag tagttgacaa agtcaatctc catgtaaaaa aaggccgaat atatggtctg
    33421 ttaggacgca acggagctgg gaaaaccaca attatgaaaa tgattttagg attgacttca
    33481 atcacgtatg gcgaggtaga tgtatttgga gagaatatta agggacgtga aaaacaagta
    33541 tatccccgta tcggtgcaat tatcgaaacg ccaggatttt atcctaatct gacaggaacg
    33601 gagaacttgg agatttttgc aaaactgcgt ggtacagccg cccctaattc tgtaaaaaga
    33661 gcattggaaa ttgtaggctt accttataaa aataaaaagc tgtttagtaa atattcactt
    33721 ggcatgaaac agcgacttgg tattgccaac gctatcctgc atgacccaga actgttagtt
    33781 ttagatgagc caaccaatgg tcttgaccct attggtattg cggagatgag aaagtttatt
    33841 agggacttga gcgttgagca tggaaagaca attcttattt ccagtcatat tctttcagaa
    33901 atctcattgt tggctgatga tattggaatt atcgaccacg gtgttctttt ggaagaaagt
    33961 agtatgagtg aactagaaaa gaaaaacagt aaatatatcc tgctacaagt ttcagatatt
    34021 ccgaagacat cacttatttt agagcgtcaa tttaacacga aggattattc cgttcaagat
    34081 gactacacgt tacaccttta taacactaat ctggatatgg gtgaaatcaa taaagcactt
    34141 gtaatgcaga atgtaactgt tattagttct caactttgta atgatacttt agaggactac
    34201 tttaaaaaaa tcacaggagg agagggcatt gcttaaactt attcaaacag aatttcttaa
    34261 actgcgtcgt aggaaactta tttggctgat gttgttgtct gctctcatta tgccgttttt
    34321 cgcactacta tacttcaatt attttggtaa aacaggtgtt gagccaataa agttttataa
    34381 atggtctgca tttggttata cactttggat tattttgcct gttgttttgg gtatattttg
    34441 caccatgctt atacacgaag aaaaccagta tgatatgctc aaacagcttt ggattgtacc
    34501 tattagtaaa atgggatact tttttagtaa atttttcgtt gttttgattt actctatttg
    34561 ttttatgctg attacagccg ttgcttctgt gttatttagt gtactttctg gatatgtcgt
    34621 atttagctgg gcaagcattt tatatttgtt gaaaaaatgt ttagaaattg gtgtaatcac
    34681 tgcttttgta atgttgccga ttttggcaat cgctacatct caaaaaggat acattctccc
    34741 aatttgtatc acattggtat atgccttttc cggttttgtt ataatgacaa taaatatgta
    34801 tttacaccca ttatccagta tgggagttat tatcatgaga aataaagaca ttccaggtct
    34861 tatattttca caggaaatta atgtcccatt agcatttctt tgtatttgta tttggagtat
    34921 tgtatcagta ttgtttgcaa atattgcact aggaagaaga aagtgaggtg tagataatgt
    34981 ggaaattact ttgggctgaa tgtcaaaaaa ttcgtcgttc aaaaattgtt tggattacag
    35041 tttttgcatc cattatgata gcagtcatag tttttgcagg ggggcaggat gtatataatg
    35101 acccagattt acattatggc ttaaagagcg tttatgacgg ttcacggtat atagacaatg
    35161 caggttggta tatggacgca gtacaacctt ggtcaacttt ttttgttctt cctgctgtca
    35221 ttgccctatt aggaagctat attatttgtc gtgaagaaga ggaggacact attaaatccc
    35281 tgcgtcttat tcccataaat gaaacaaatt taaccatagc aaaaatgatt attactttta
    35341 tattcagtat acttctttat ttgttactct ttactataac atttttgact gaatccgttt
    35401 tacatttttc cgatttatcg acaaaattgg ttttaagttg catgaaagag tattttcttg
    35461 atggcatagg tgtattcttt gcaatttctc ccattattgc cttggtatcg cgcatgaaaa
    35521 aaggatattg gcttgcatta gtattcacag aagtttattc tattgctgga ctatttgcgg
    35581 gtatgtcaaa tgtcttacaa accttttttc ctattgcggc aatatttaat atttctggat
    35641 actatataac tactggagaa aaaattatgg ggagtatcat gagtttatta ctttgtgcct
    35701 gcctttctgc atttatactt aaagggctaa aacacaatga aaaaaattga aataggtata
    35761 tcaaaatgaa aaagataaaa aaactgatta ttctaattgt attcttgttt ctttttggca
    35821 tgttacactg ttatactgaa tggaaattca acaataaccc acctaaaggg gtaatagaaa
    35881 gaataaatga gtggactgat aagattggac tggattagta acttatgtta attatagtga
    35941 atgtaatatt agcaagttat tgtttcgtat tttaatacta taactttaaa tatttggttt
    36001 taatcttcca aacgaatata aaaaaactgt cacagaggca acatattttt gaatatttat
    36061 aaagtggtag ttttgatttt caaagctacc gccttttctt ctatataagc aaacttatcc
    36121 tcaacaaagt gtaaaacatg gtgcatttta agaaaaaagt accacagaca caagcaataa
    36181 aagaaacgcc gagaacctac aaaaacgtag tgcttatgca gatttacgac gccttctatt
    36241 ttctcaaccg tcttaatgac cttgtacaaa agaaattttt ctaatatttt ttataatctt
    36301 atcttgtgat gttttttatg tataaatgac ttaataacaa atccaataaa aattatggat
    36361 attggtataa ttagaacgag gaatttataa tcattataat gtgacattat tcttacccaa
    36421 ctctcagaaa aaatatatcc tatactgatt aatgaagtat tccatatggt tattcccacg
    36481 atagatgata ttacaaaagt tataatattt tgacctgcta tacctgctat aaaagatatg
    36541 tatgttctac ataaagggat taatctacat acacaaacag aaatagaaga gtatttatta
    36601 aaataatctt gagatgcaaa tattcctttc tttgcctttg gacatatctc tattatttta
    36661 tttatgatag atttacctcc ccaatttccc actgtataac aaaatatcga accaagtata
    36721 cctgcaatta tacttaaaat taaaattgtc agaaatccaa aatgatttct tgcagcaatt
    36781 gctccacata aaggtaaaac aacttcgcta ggtaatggaa aacaagcata ctcaatcata
    36841 attaatacaa atacagagat taatccatat tgtaaaataa aactattgat tacagacact
    36901 tacttatcta tctcctttct tatttaaagg ttttaagggt acatgttcta aggattctca
    36961 ccctatattt aaagaatcaa tctctttaaa ctattatatg tatttacaca aagcatttat
    37021 aacatacttt aaaaatagta ccttaaaata agaaacttaa taaataaaaa aagattacgt
    37081 ggctacgtcc tactctccca ggcagtttcc caccaagtac catcagcgct agagagctta
    37141 acttctgtgt tcggaatggg aacaggtgta tcctctctgc tattgtaacc acataatctt
    37201 tatgcttaat atttccatta agtttttaat aagtttatag aacctagtaa tactagtttc
    37261 tttagagtta gcactctaaa aactacacat atatc
//
LOCUS       DAEPIN010000038        36962 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.038, whole genome shotgun sequence.
ACCESSION   DAEPIN010000038 DAEPIN010000000
VERSION     DAEPIN010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 36962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36962
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.038"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            complement(604..744)
                     /locus_tag="KR250_002078"
     CDS             complement(604..744)
                     /locus_tag="KR250_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0068013.1"
                     /translation="MENLMMSIITGVIASYIYDKLKSHSERPTKSGWELNIKFQKSKS
                     SK"
     gene            complement(1002..2162)
                     /locus_tag="KR250_002079"
     CDS             complement(1002..2162)
                     /locus_tag="KR250_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp27"
                     /protein_id="HBH0068014.1"
                     /translation="MKLNKTLLAIGMSVSLLIASCPTSNALSSINSIKGTNKYETAGL
                     IADKQNYDIAILINSENGLSDGLSASGLSGAKNAPILLTKKDTIPSETLKRLEKVKEV
                     YLIGGKNSIGASVQKILSDKKISINRISGTDRIDTSHKVAKEIGSVSAVKKVILTNAF
                     KGEPDAMSVASIAVRDKSPIVLTDGNTTSFATDGLETYAIGGKVSISDKLINETKATR
                     IGGVDRYDTNKQIINKFYNGSKEFYIASGTDLVYSLVGSTIAQKTPIVLVGNGSNKSI
                     LKNASKITAIGNIDDVVIKQCLNITNNVGDSNTGIVKDTNSNNTNKPSNEPSSNNPGL
                     TDKSTVYVTPNGKSYHLTKNCSTLKRSKTINKVTLKQAKSQGKIDPCNVCVK"
     gene            complement(2333..2536)
                     /locus_tag="KR250_002080"
     CDS             complement(2333..2536)
                     /locus_tag="KR250_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4236 domain-containing protein"
                     /protein_id="HBH0068015.1"
                     /translation="MGFNFRKSINLGGGLKLNLGKKSVGISAGVKGARVSVNSKGRKS
                     ATLSIPGTGISYTKTSTKKRKPK"
     gene            complement(2623..3657)
                     /locus_tag="KR250_002081"
     CDS             complement(2623..3657)
                     /locus_tag="KR250_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHYH domain-containing protein"
                     /protein_id="HBH0068016.1"
                     /translation="MFKKKLIAVAMSITMISVGSFSYAHSGRTDSSGGHRDNKNKSGL
                     GSYHYHCGGYPAHLHNNGGCPYTGGGSSSGTATSVNNEEKQKRSVGEKGYNQGYEDGY
                     KGNYSSSNYSGDYSDTYESKYSEGYEKGKAKLEEEEKEAKETGYNLGLTGAKSNNTYE
                     KEVLKNAYDTGYSTGYNEYKTKKIEEYKAKGIEDSNKDKEKMTFEENIDSKFIDAYNN
                     AYDEIQEQLKNDYTTQGFESAIKGERFDTSTIGNVKYANWFKEGYDNGKVKLPKAKES
                     VYNQGYNEEDFSIPDEMKSIETKLKGVYDEGLEKREEEKSRNATYGVGAGTAAVVAGV
                     VYKKKKRKKI"
     gene            complement(4179..5540)
                     /gene="rlmD"
                     /locus_tag="KR250_002082"
     CDS             complement(4179..5540)
                     /gene="rlmD"
                     /locus_tag="KR250_002082"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HBH0068017.1"
                     /translation="MSVKKKDVIEFEVDKMEFGGTSLSQVGDRVIHMKGGISGQKVRA
                     GVKKVRSKKAEVKMIELLENSPLETEETCKHFRECGGCTLLSVPYEKQLEIKEKQVMD
                     LFLKQDLFGFQFLGIEGSPENKYYRNKMEYTFGDEVKNGPLTLGLHKKGKHIDIQTVE
                     ECMLVDEDFSKILVSSVEFFNEKKLPYYRTMNHKGYLRHLVVRKGIHTNEIIVNIVTS
                     SQEDFDMYEFKDMLLGIDLKAELVGVLHTINDGLADAVQCDELRVLYGRDYIQEEILG
                     LKFKISPFSFFQTNTKGAEVLYSIARDFIGDYNDKVVFDLYSGTGSIGQVMAGAAKKV
                     YGIEIVEEAVVAANENAKLNGLTNCEFIAGDVAKVVKDLKDKPDLIIVDPPRPGIHKD
                     AIRDICGFGAKEIVYISCNPKSLVVDLVDFKGYGYEIKMVKCMDMFPNTPHCETVVKL
                     IRE"
     gene            complement(5567..6289)
                     /locus_tag="KR250_002083"
     CDS             complement(5567..6289)
                     /locus_tag="KR250_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HBH0068018.1"
                     /translation="MAKKQRIDKILSNLGYGSRSEIKKYCKQGSVVVNGSEVSNPGTQ
                     VDTENDEILFNGEEVIYREYIYLMMNKPDGYISATTDKYDPTVLDLIDLSYLAFEPFP
                     VGRLDKDTEGLLVLTNDGKLSHRVLSPKKHVPKTYYAKIDGVVTEEDVEAFLEGVVLD
                     DGYKTMPSQLNILKSDDESEIELTIHEGKFHQVKRMFESVGKKVVYLKRLSMGNLKLD
                     ESLELGEYRELTDEEVKLIEER"
     gene            complement(6289..6618)
                     /locus_tag="KR250_002084"
     CDS             complement(6289..6618)
                     /locus_tag="KR250_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1904 domain-containing protein"
                     /protein_id="HBH0068019.1"
                     /translation="MPQIKIRGINENDICKISEKMINDLVEAVKCPRDYFEIECIKSV
                     AIRDGKIADVYPFVEVAWFDRGQEVQDIVARIITDSIRNNLDVESMDLAFTVFEKEKY
                     YENGEHF"
     gene            6948..7874
                     /gene="fba"
                     /locus_tag="KR250_002085"
     CDS             6948..7874
                     /gene="fba"
                     /locus_tag="KR250_002085"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HBH0068020.1"
                     /translation="MALITTKEMFKKAYEGGFAIGAFNISDLEQLQGVLKAAKAKNSY
                     VMIQASMSAVKYAGPHTLVEMVKAASDEIGVDVALHLDHGPNMDAIKTCIDAGFSSVM
                     IDGSHFDFEENVRITKEAVEYAHSKGVVVEAELGVLAGTEDDVTSDVHKYTQPAEAVE
                     FVERTGVDSLAIAIGTSHGAFKFKGEAKLRFDILEEIQSKLPGFPIVLHGASAVDQNA
                     VATCNEFGGNIAGAKGVPVDMLRKASSMAVCKINMDTDLRLAMTAAIRKFLAENPKEF
                     DPRKYIGAGRDAIQAVVESKIDDVLGSANSIN"
     gene            complement(8009..9640)
                     /locus_tag="KR250_002086"
     CDS             complement(8009..9640)
                     /locus_tag="KR250_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBH0068021.1"
                     /translation="MDNNLIGLIEHVENPAILCKESGEIIYCNHLIDSIFSFLDIKKP
                     RNINELDSNFDKTEILTDSKKKIAFRELRMTAHIYNMKDNNNENNIVYLFEKSLISDK
                     VIEDIIEHIDEVVVVFNKDGVIEKMNTVSDEILPFKRTEVLGRNITDLVRQGLVEEPI
                     ILNMLKVKKKIYRNIVYPDGKLIAYTAVPRWDSKGKLTGGVLTGRDISRVIKLESQIK
                     YSDISEDTEYISQSKIMDNIKKVVKRAAASDSSIFINGESGVGKEIIARTIYKYSSRR
                     DKPFIAINCGAIPNELLESEFFGYEEGSFTGAKKKGKKGLFEEANGGTIFLDEIGELP
                     MQMQKKLLRVIQENTITRIGGSKPIKIDVRYISATNISHEDLRNNLKFRQDLYYRLSV
                     IPVKIPPLRERKEDIVPLVNYFLKLYNEKYNREVEVSPKVIELLEEYSWPGNIRELKN
                     IIERFVVLSAKNVIGEDEFNMLINLDMIDNETDDLSPIVVNGIMNLNDAYKIVDQIMI
                     SKAINKYGSITKAAEVIGIYPSTIHRKIKSGHIHV"
     gene            complement(10123..11160)
                     /locus_tag="KR250_002087"
     CDS             complement(10123..11160)
                     /locus_tag="KR250_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HBH0068022.1"
                     /translation="MNDIKDLSSYKNVWIFAEQREGKIAPVVIELLGEGRKLAKEVDA
                     ELCAILLGKDVDGLAKELITFGADKVYVADDALLEKYTTDAYTKVIKDAIDEIKPEIM
                     LFGATHIGRDLAPRIASRVGTGLTADCTKLEIDPEDKKIKQTRPAFGGNIMATIICPN
                     HRPQMSTVRPGVMDKAEKDETRTGEVIALDYKITQDDIRTTVLETVKTKKDLVSLTDA
                     NVIVSGGLGLGGPEGFEMLKKLADKLGGVVGSSRAAVDAGWIDHSHQVGQTGTTVKPN
                     LYIACGISGAIQHLAGMQSSDFIIAINKNPAAPILEIADYGVVGDLHEIVPMLIEKLD
                     SVDDLLEAIKA"
     gene            complement(11179..11964)
                     /locus_tag="KR250_002088"
     CDS             complement(11179..11964)
                     /locus_tag="KR250_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012703997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HBH0068023.1"
                     /translation="MKILVCVKQVPDTNEVRINKETGTLIRDGVPSILNPDDANALEE
                     ALKIKDEHDDVTITVITMGPPQADFMLRECLAMGADDAILLSDRAFGGADTWATSNTI
                     AAGISKVGDYDIIFAGRQAIDGDTAQVGPQIAEKLDIPQVTYVQDFKIEGKDIIVQRQ
                     LEDGYELIKVSTPVLLTAVKELNTPRYMSVDKIVKAYKHDVKVWTIDDLDVNKEEVGL
                     KASPTKVFRSFTPEPRGKGEILEGKADEIASKIIIGLKQKHII"
     gene            complement(11997..13130)
                     /locus_tag="KR250_002089"
     CDS             complement(11997..13130)
                     /locus_tag="KR250_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003483287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HBH0068024.1"
                     /translation="MLYNKEQELLRKAVRDFVSKELDTLPAEMDKTGVMPKELIKKLA
                     DAKFISSNIPEEYGGGGAGYVSYAIVMEEIARRCASTATFVTAGSSLASLPILYNGTE
                     EQKQKYLKGIATGELIGAFGLTEPGAGSDAGGQQTTAELVGDHYILNGRKTFITNGPF
                     CDVAIVIAVTDRSKGLRGTSAFIVESKWDGFSTGAHEDKMGIRGTETSDLIFENVKVP
                     KENLLGKEGQGFKIAMGTLEVGRIGVAALALGIAQGALDEAVKYTKQRVQFGKPIAKF
                     QNTQFTIADMETKVCAARGLVYDAAQKRDAGMRVAQESAMAKYYASEIANEVAYKALQ
                     LHGGYGFIKDYEIERMYRDARIVSIYEGTSEVQKMVISSNVLK"
     gene            complement(13178..14305)
                     /gene="hadC"
                     /locus_tag="KR250_002090"
     CDS             complement(13178..14305)
                     /gene="hadC"
                     /locus_tag="KR250_002090"
                     /EC_number="4.2.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(R)-2-hydroxyisocaproyl-CoA dehydratase subunit
                     beta"
                     /protein_id="HBH0068025.1"
                     /translation="MEAILSKMKEVVENPNAAVKKYKSETGKKAIGCFPVYCPEEIIH
                     AAGMLPVGIWGGQTELDLAKQYFPAFACSIMQSCLEYGLKGAYDELSGVIIPGMCDTL
                     ICLGQNWKSAVPHIKYISLVHPQNRKLEAGVKYLISEYKGVKRELEEICGYEIEEAKI
                     HESIEVYNEHRKTMRDFVEVAYKHSNTIKPSIRSLVIKSGFFMRKEEHTELVKDLIAK
                     LNAMPEEVCSGKKVLLTGILADSKDILDILEDNNISVVADDLAQETRQFRTDVPAGDD
                     ALERLARQWSNIEGCSLAYDPKKKRGSLIVDEVKKKDIDGVIFCMMKFCDPEEYDYPL
                     VRKDIEDSGIPTLYVEIDQQTQNNEQARTRIQTFAEMMSLA"
     gene            complement(14305..15531)
                     /gene="hadB"
                     /locus_tag="KR250_002091"
     CDS             complement(14305..15531)
                     /gene="hadB"
                     /locus_tag="KR250_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(R)-2-hydroxyisocaproyl-CoA dehydratase subunit
                     HadB"
                     /protein_id="HBH0068026.1"
                     /translation="MSEKKEARVVINDLLAEQYANAFKAKEEGRPVGWSTSVFPQELA
                     EVFDLNVLYPENQAAGVAAKKGSLELCEIAESKGYSIDLCAYARTNFGLLENGGCEAL
                     DMPAPDFLLCCNNICNQVIKWYENISRELDIPLIMIDTTFNNEDEVTQSRIDYIKAQF
                     EEAIKQLEIISGKKFDPKKFEEVMKISAENGRLWKYSMSLPADSSPSPMNGFDLFTYM
                     AVIVCARGKKETTEAFKLLIEELEDNMKTGKSSFRGEEKYRIMMEGIPCWPYIGYKMK
                     TLAKFGVNMTGSVYPHAWALQYEVNDLDGMAVAYSTMFNNVNLDRMTKYRVDSLVEGK
                     CDGAFYHMNRSCKLMSLIQYEMQRRAAEETGLPYAGFDGDQADPRAFTNAQFETRIQG
                     LVEVMEERKKLNRGEI"
     gene            complement(15546..16346)
                     /gene="hadI"
                     /locus_tag="KR250_002092"
     CDS             complement(15546..16346)
                     /gene="hadI"
                     /locus_tag="KR250_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyisocaproyl-CoA dehydratase activator
                     HadI"
                     /protein_id="HBH0068027.1"
                     /translation="MYTMGLDIGSTASKGVILKNGEDIVASETISSGTGTTGPSRVLE
                     KLYGKTGLAREDIKKVVVTGYGRMNYSDADKQISELSCHARGVNFIIPETRTIIDIGG
                     QDAKVLKLDNNGRLLNFLMNDKCAAGTGRFLDVMAKIIEVDVSELGSISMNSQNEVSI
                     SSTCTVFAESEVISHLSENAKIEDIVAGIHTSVAKRVSSLVKRIGVQRNVVMVGGVAR
                     NSGIVRAMAREINTEIIVPDIPQLTGALGAALYAFDEAKESQKEVKNI"
     gene            complement(16385..17584)
                     /gene="hadA"
                     /locus_tag="KR250_002093"
     CDS             complement(16385..17584)
                     /gene="hadA"
                     /locus_tag="KR250_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocaprenoyl-CoA:2-hydroxyisocaproate
                     CoA-transferase HadA"
                     /protein_id="HBH0068028.1"
                     /translation="MLLEGVKVVELSSFIAAPCCAKMLGDWGAEVIKIEPIEGDGIRV
                     MGGTFKSPASDDENPMFELENGNKKGVSINVKSKEGVEILHKLLSEADIFVTNVRVQA
                     LEKMGIAYDQIKDKYPGLIFSQILGYGEKGPLKDKPGFDYTAYFARGGVSQSVMEKGT
                     SPANTAAGFGDHYAGLALAAGSLAALHKKAQTGKGERVTVSLFHTAIYGMGTMITTAQ
                     YGNEMPLSRENPNSPLMTTYKCKDGRWIQLALIQYNKWLGKFCKVINREYILEDDRYN
                     NIDSMVNHVEDLVKIVGEAMLEKTLDEWSALLEEADLPFEKIQSCEDLLDDEQAWAND
                     FLFKKTYDSGNTGVLVNTPVMFRNEGIKEYTPAPKVGQHTVEVLKSLGYDEEKINNFK
                     DSKVVRY"
     gene            18261..19259
                     /locus_tag="KR250_002094"
     CDS             18261..19259
                     /locus_tag="KR250_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HBH0068029.1"
                     /translation="MKILVFGARDYEEPVIKKWSEEHKDVQVDIYPENMTEENVVKAK
                     GYDGISIQQTNYIDNPYIYETLKDAGVKVIASRTAGVDMIHFDLVNENGLIVTNVPSY
                     SPNAIAELAVTQAMNLLRKTPLVKKKVCEGDYRWIAELLGTEVRSITVGVIGTGKIGA
                     TSAKLFKGLGANVIAFDQYPNSDLNDILTYKDSLEDLLKEADLITLHTPLLEGTKHMI
                     NKDTLAIMKDGAYIVNTGRGGLINTGDLIEALESGKIRAAALDTFETEGLFLNKKMNP
                     GELTDPEINKLLSMEQVIFTHHLGFFTSTAIENIVYSSLSSAVEVIKTGTATNRVN"
     gene            19621..20148
                     /locus_tag="KR250_002095"
     CDS             19621..20148
                     /locus_tag="KR250_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04002 family protein"
                     /protein_id="HBH0068030.1"
                     /translation="MEKMNKVTSKKTTYIVTSALFASIICLTIAYILHIPVGGNNGYV
                     HIGDAFIYLAATILPTNYAIAASAIGAGLADLSTGAAIWVIPTIIIKPILVLFFTSKS
                     DKIINKRNIVASVVAGIVGLVLYMFAEGIIIGSFTSAFVMSLLGLLQPIGSFIVFIIL
                     GMALDKLDFKKRYFN"
     gene            20175..20771
                     /locus_tag="KR250_002096"
     CDS             20175..20771
                     /locus_tag="KR250_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04100 family radical SAM protein"
                     /protein_id="HBH0068031.1"
                     /translation="MNILYTIENSIYVNITNTCPCSCVFCIRNEKDEVANSGSLWLEH
                     EPSVDEVKEAFNKYNLDDYDEIVFCGYGEPLMRINELIEVAKFIKEKSSIKIRINTNG
                     LSDLIHNKKTAILLKNVIDAVSISLNAPNKEAYNRVTQPKFGEKSFDYMLDFARDCKK
                     YIKEVAFSVVDEISPEEIEESKQLAKKLDIPLRVRHKN"
     gene            complement(20836..22419)
                     /gene="asnB"
                     /locus_tag="KR250_002097"
     CDS             complement(20836..22419)
                     /gene="asnB"
                     /locus_tag="KR250_002097"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HBH0068032.1"
                     /translation="MINLEEKYLTRKDTYMIKILEYNGHIRNWKVLCEELGIDSSLCR
                     DERERAILVEAYKTWGYDMANHMHGMFAFALWDTVEKKLFCLRDQFGVKPFYYYETEN
                     GKLLYGSSIRKIMEQPGFVKELNEDMLQIYMSFTYVAGENTFFRGVKKLMPGHYLVYQ
                     NKTVCIGRYWKPEFHPDHSKSIEEWTDEIHTTLQKIMPEVKSENETAELFLSGGVDSS
                     YILAMSDIEKTGSCGYEEERFDESKLAQQTANILGCKNSRYIITPEEYFASVPYVMYH
                     MEQPTGDASAIVFAIECKAAAENTNICYSGEGADEFFGGYNIYHKAECYGENLKTFYI
                     GNTNIMNEGEKRKILKKYNPDVLPIEVVKGIYEETEELDTLTNMSNVDIQIWLEGDIY
                     FNIDKMSTAVGLEVRMPLTDTRIFDIASRIPSEYKVKGKENKIAFRTAASKTLPEEIA
                     FRNKLGFIVPIRIWMADDQYNKDIRDKFNSEIADKFFNIDEINEIFNDYISGNSDNWR
                     KIWMIYTFLVWYEIYFVKC"
     gene            complement(22985..23821)
                     /locus_tag="KR250_002098"
     CDS             complement(22985..23821)
                     /locus_tag="KR250_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBH0068033.1"
                     /translation="MNYIIKKVLNSSVVLVHDTKDNEFILLGKGIGYGKKTGEYICGS
                     NDNQMFVPVENTKSKQFLELMDDIPVDILKITQEIIVEAEKLLNSSFNKNLYLILADH
                     FNFAIERMRKGIKITNRVFWEIKNYYPNEFKVGMVAIYMVEERLGIQLPEEEAANIAF
                     HFANAMASDGNSYDTIKYAKVIGEIINIFVFSLNRTLDKKSMHYMRFITHIKFFVERF
                     FSDSMLSSGDDLLFNQMKRSNPKEMLIALKVRDFLEKKYGKKLTNEEIAFLVVHIARV
                     KQ"
     gene            complement(23845..25311)
                     /locus_tag="KR250_002099"
     CDS             complement(23845..25311)
                     /locus_tag="KR250_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HBH0068034.1"
                     /translation="MRKFPEGFLWGGATAANQFEGGWNLGGKGWSVSDVAKAHFDADV
                     KDYKSNNEITTKDIEEGLAHPEDEVNYPKRHGSDFYHHYKEDIALMAEMGFKTYRMSI
                     AWSRIFPNGDDKEPNEEGLQFYDDVFDELISYGIEPLVTMSHYEPPLNIVLNYDGWYS
                     RQVINMFVRYVETICERYKNKVKYWLTFNEVDSMIRHPYTTGGLVRDRFKDKNFEEVI
                     FQAMHHQFVASALATKICHEIIPNSKVGCMLTKLTYYPYTCRPEDVLATQRKMRSIYA
                     YSDTQVFGEYPVYLLSYFKNNNIQIVKEEHDDEIMKKYPVDFISFSYYMSSCEAADTT
                     GLDITPGNTLLAVKNPYLEMSEWGWQIDSIGLRISLIELYDRYRKPLFIVENGLGAKD
                     ILTEDKKVHDQYRINYLKEHFKCMLDAIIEDGVELWGYTSWGCIDLVSESTKQMSKRY
                     GYIYVDADDYGKGTYNRYKKDSFYWYKKVIESNSIDFK"
     gene            complement(25314..27176)
                     /locus_tag="KR250_002100"
     CDS             complement(25314..27176)
                     /locus_tag="KR250_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBH0068035.1"
                     /translation="MNYKKTAIDIIEQVGGSKNILALEHCSTRLRFKLIDRSKVNQDA
                     LKKVQGVMSIVNGAQFQVVIGNSVVDVYDEILKVCPLDTNDSTVKTEEKINLGTRFIE
                     FIINIFQPLIFAIAGAGILKSLLMLLAMFNILKSDSTIYTLLVAISDATFYFLPLMVA
                     VTTANVLKCNRLVAIAAVGYLLLPATTTALSEGVELFGFTIPNIAYNAQVFPAILCVS
                     FLATMEKIFNKYSPKPIRTFFVPMMSLAITVPITLTILGPLGYNVGTIFTTIILFLYN
                     KMGFLVVGLLAAFLPFMVATGMHKALIPYSINTIGKLGYEALYMTSSLAHNISESGAC
                     FAVALRTKDETLKQTALSAGISALMGITEPALYGITLQHKRAMLGVVFSSAISGTFLG
                     LFAVKGFVIVGPGLASMSMFIDPNNGNNLIFASIGFVIAILGSFIITLIIWKEDTNTV
                     ESSIGDVVEEELEEEKEVNILVTPVEGKVIDLSKVNDELFASKTLGDGVAIIPTNGNL
                     YAPCDSEVVMLFETKHAIGLRTKNGAEILIHIGINTVSMNGDGFKTFVKTGDNVKEGD
                     LLIQFDLDKISHANLDSTVMIVNNNGADYAYKVLNQSYGNVKKGSILFDVKGGI"
     gene            complement(27423..28286)
                     /locus_tag="KR250_002101"
     CDS             complement(27423..28286)
                     /locus_tag="KR250_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HBH0068036.1"
                     /translation="MILSYFALLIRVVLLAFERVVVRLLGDEEGDIYKNIASSFLFFF
                     IGGVCLLPFSIMDRVSDWSFLIPCYISSLVYSIGSVAYVTSLATGEVSLVTPINSLNS
                     LFLLLLSVIFLGEGLSLAKVAGIIIMIGGVFILKKVSSFLKNVSVIFNNLPCRLMFLY
                     IFLQSVGRVLDKAFFVQVSPILYSTILYFFVGLNLFIFLAFKKKQKIICEIFSNKKGL
                     SILSGAINGYSYLALLIALNNLELSIAEPFSQVSMIITLILAHFIFKENIKEKIPGSI
                     LILIGGWLLLL"
     gene            complement(28341..29273)
                     /locus_tag="KR250_002102"
     CDS             complement(28341..29273)
                     /locus_tag="KR250_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HBH0068037.1"
                     /translation="MNNKKAVFFFCSDLKKDPVASNVIKCCEEIMDLNQTDIVIDDNF
                     VLEFKDKNNNLFHFVKTKDVISHNYKYYLPILNRHFRDYDFAGVVNWHEGENAPEHIL
                     TAHTIGDVPTGEFGNSNPRYFKNLINAIEDIKNENLLDEFTTLTEATHWSGTTYGEES
                     KLITEYSVPMLDIEIGSSSDSFNNSIAIQVLAKSLIRVFDCDEPLKTLLCVGGVHFEK
                     SFSDIIKNKEYNISIGHVLPNQWIVSGMYDDESGFEKLEKCINSIEGGIDCIVFHDKL
                     KGTYKEQCRKLGEKLNVPVFKHKILKNPKDLPIW"
     gene            complement(29359..29910)
                     /locus_tag="KR250_002103"
     CDS             complement(29359..29910)
                     /locus_tag="KR250_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH0068038.1"
                     /translation="MQSLNEIIAENLKKIRKEKHLSLDKIAQLSGVSKSMLSQIEKCE
                     VNPTISTLKKITNGLKISFTSLMERQESDIELIQKSDIDHFIEDNGKYISYPIFPFDS
                     KRRFEIFMIEIEEGGNLDSNAELPGTQEFITVFSGEVTIKINGEDYIVSSGNSIRFKA
                     DVSHIYRNSGKGIAKMSMVVYYI"
     gene            complement(30139..30630)
                     /locus_tag="KR250_002104"
     CDS             complement(30139..30630)
                     /locus_tag="KR250_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0068039.1"
                     /translation="MIDLKIENRKGKIHVKSSEIKDILELRPDFEYVQDISNTINQEN
                     ILVFDCQLTGNSFDMDDLDIEEILEELGEEIDESYFSVLFEDVRAYLKDATDEIEADL
                     QDNYLFDNLRCYFDIYNINQEFTDFKFVFLVSFKDIKISSLANLAKIVSKRQLIGASK
                     FYS"
     gene            complement(30658..31218)
                     /locus_tag="KR250_002105"
     CDS             complement(30658..31218)
                     /locus_tag="KR250_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBH0068040.1"
                     /translation="MNKLNICIDIDGTITSPYHFLPYLNDIFNKNITEAECITHNWEE
                     LYGSGIRDVYAEFNNKYIHSYDEAKIVEGATEVISTLSKGHNLSFVTARHECLTDVTK
                     NWLSRQGFSDIDVYLLGSDYKVEKARELSCDIFIEDNPLNSVQLADDGVKVILLDTNY
                     NKDVKHQNIVRVSNWIDIEKIIKQGI"
     gene            complement(31370..32527)
                     /locus_tag="KR250_002106"
     CDS             complement(31370..32527)
                     /locus_tag="KR250_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HBH0068041.1"
                     /translation="MNKQSIGKLNMFFMGITQRNLDNRDFFIELEVIFKSGRKEFKGI
                     AIEEDNKYKFNFKGKSDLYTFNELLKNISKEAENYDGLVFKYVERGTIVVIEGDNKKV
                     NVKYLDNKEEVPKIDEFTASQIKNRDYYVKVGQANALLKEIGVLTKDGKIKNDKIRKY
                     NQIDHFVELIDSILKEIKDKDCITILDCACGKSYLSFVLNFYIKEVLKKNCYFIGIDY
                     SDVVIEASKNMAKNLGYKNMSFIKEDLTNYTPNRDVDLVISLHACDTATDMAIGLGIR
                     AKSEAIVVVPCCHKELLGQYRYEAMEPILKHGVFKARFADLITDGLRTLLLEGNGYDT
                     SVVEYISPLDTPKNLMIRAIKTKTNNDKALKEYKELKSQFGVEPTLEKLIY"
     gene            complement(32564..33505)
                     /locus_tag="KR250_002107"
     CDS             complement(32564..33505)
                     /locus_tag="KR250_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0068042.1"
                     /translation="MRWILVFIFLYLIPLVVIFRNYKNFKRSCIYSSIYVVLATTIMI
                     SNIYMSGLNKIKESMYYHNYISDETYDEKYVSNFKEKESTDLNQDNKKNEEISNNKNL
                     NNKQDRNIQNKESNEEGSNDIKEKNNSDSNVSVNNKSEKQEDNTKENNKYTSKEKNSE
                     DIKKIDMESIGIFKKEIYNIERAALVPMRECIPYTKDIAKNITKLSSIKKDVTNARNK
                     CKEVVKEYENMQIPELSKEKYTETLSNARDDVKKAYELREKSMESALKLINTKSPKYI
                     GKITEYLDLSDKQIESFKNRLNDLKEEINNSETNNIN"
     gene            complement(33592..34284)
                     /locus_tag="KR250_002108"
     CDS             complement(33592..34284)
                     /locus_tag="KR250_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBH0068043.1"
                     /translation="MSLYAIGDLHFSTSVNKPMNIFGSNWDGHEKKIIDNWKEVVKEE
                     DMVLVLGDTSWGINLSEAKKDLDIISKLPGQKILIKGNHDYWWTTVTSLNKLYEDMRF
                     IQTNFYEYKDYAICGGRGWICPNDVKFDETDEKVYKREEHRLRLSLESARKSGHSKII
                     VITHYPPTNDKLEESLFTKLFEEYNVEKVIYGHLHGKESFKMGLKGIRNGVEYTLASC
                     DYTEFNLIKVHD"
     gene            complement(34393..35205)
                     /locus_tag="KR250_002109"
     CDS             complement(34393..35205)
                     /locus_tag="KR250_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HBH0068044.1"
                     /translation="MEYEKWNEILAPYEHAVEELKIKFKNIRKEYLAKGEYSPIEFVT
                     GRTKKISSIISKLKRINAKDIETEIDDIAGIRIMCQFVEDIYAIVDLIKVRNDMTIIG
                     EKDYITNYKDSGYRSYHVIIKYPINSIAGSKEIICEIQIRTLAMNFWATIEHSLKYKY
                     DHYIPETLAERLRRASDAAFLLDQEMSEIREDIMKAQAMYQMKSIAIRDTLNRIQELY
                     DLGDTHKAMQYQRKLDRTETDKNITEILELKQEIDILLEQYKDTKVDDKVVQ"
     gene            complement(35256..36167)
                     /locus_tag="KR250_002110"
     CDS             complement(35256..36167)
                     /locus_tag="KR250_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBH0068045.1"
                     /translation="MNLTKVKGNTFFIKGGTNTGIYVFEDNTALMIDPGLCGSRPRRV
                     NKVLDENNIQVRYIINTHGHDDHYGACNQFKEYYKDVCIMSSEYDKLYIENPELFSKY
                     IVGGKSNVFMDNILKNKMLDDIKIDKFIEAGKFELNNEMFDIIEFQGHTPGSIAVLTK
                     DNVIFVGDLLIGDEILSKYDFLFLYDIQEQINSLEKLKNIDFEYLVLGHGKQVISKED
                     SYKLIEKHLNALKKYLYQIRELLVEPKSLEILLKNIINNNNLSNNYKEYHFFKSSLVS
                     VISYLVDLEEIGYILENGELLYYTKTK"
     gene            complement(36226..36957)
                     /locus_tag="KR250_002111"
     CDS             complement(36226..36957)
                     /locus_tag="KR250_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HBH0068046.1"
                     /translation="MTSIKNKDILNCIDYSIKNKLFDKLNDVYKSLPVGNCSGCGNCC
                     MESVGINLIEFLNIFCYLEDRVDLKKSCISKILDYYFEEYTRKNPCPFKDTNNRCLIY
                     EVRPLNCRMFGHWKKEDYNKNLDNVIEKNNNYRELMKKQYGFEINDEVVNYRIDYCDR
                     FIPNKDYLSKSKRLSFFDELMILDSNIYSSGNIDIDFRDRGIVEYFIESLLYRNLAYN
                     VKIRISKEYKIRKRTIDRIKRIVLV"
BASE COUNT        11769 a         6646 c         4068 g        14479 t
ORIGIN      
        1 aattgtggta ttaatgttta aataaccaac tttttctaaa ataaccttta taggaatata
       61 tgagttcata agtaaagata gcgaaataag aaaccagttt ggtatgtaaa atctagattt
      121 ttaattaaat tatcaagatt atatattata gtatcaaaaa tagaaaatta aaattaaatt
      181 ttttataata aattgtatac attataaatt atattaaatt tttaatttaa gtaatagtat
      241 atgtaatctt tagtcatctt cttaccagtt ttttatccac ttttctaatg aagttatttt
      301 ttcatatgtt aattcctctt gaaataatga tactaaaaga ctttttataa aattataact
      361 taaaagttta tgtatttttt tgatatgaat ttatagtact tatcttcttt aattgagatt
      421 gtgtatattt aactaaacat tacaacaata attctatgaa atcaaatcaa ttatttacat
      481 atttttttag aacaataata agtatatatt cacaaaattt tgtgttaata tttttaattt
      541 gtgatataat aaaagcaagg aaacatattt tacttaactt tgagtgatgt ttccttcaac
      601 aaattatttt gaagatttac ttttctggaa ctttatatta agttcccagc cactcttagt
      661 tggtcgctca gagtggctct ttaatttatc gtaaatgtaa ctggctataa caccagttat
      721 aatactcatc attagatttt ccatagtttt cacctcctct cataagaaag tgaagaaact
      781 tccatggaaa caccactcat agctttttca aatatttatt ccttgccata ttaattatat
      841 cataaatatt ggaaacataa aattttaata cttatcgtct ttattattat tttttataat
      901 taataatttt ttttaataat acataatata aatttatatt tcttcaataa aaaagacaat
      961 cttaattaat tgtctttttt aatatatatt tggctaatac attattttac acaaacatta
     1021 caagggtcta ttttaccttg tgacttagct tgttttaatg ttactttatt tattgtttta
     1081 cttcttttta gtgtagaaca attttttgtt agatgatatg attttccatt aggagttaca
     1141 tatactgtac ttttatcagt taatccagga ttattagagc taggctcatt acttggtttg
     1201 ttcgtattat ttgaattagt atcttttaca atgcctgtat ttgaatcccc aacattatta
     1261 gttatattta aacattgttt tataacaaca tcatcaatat ttccaatagc agttatttta
     1321 cttgcatttt ttaatatact cttattactt ccatttccaa ctaacactat aggtgttttt
     1381 tgtgcaattg tagaaccaac taaagaataa actaaatcag tcccacttgc tatgtaaaat
     1441 tcttttgagc cattataaaa tttatttatt atttgtttat ttgtatcgta tctatctact
     1501 ccacctattc ttgttgcttt agtttcattt attaacttat cactaatact gacttttcca
     1561 ccaattgcat aagtttctaa tccatctgta gcaaaacttg tagtgtttcc atcagttaat
     1621 actattggag atttatctct aactgctata gaagcaacac tcatagcatc tggttcacct
     1681 ttaaaagcat tagttaatat tactttttta acagcactta cacttcctat ttcttttgca
     1741 actttatgac ttgtatctat tctatcagtt ccacttattc tatttatgct tattttttta
     1801 tcacttaata ttttctgaac tgatgctcct atcgaatttt ttcctccgat tagatatact
     1861 tcttttacct tttctaatct ttttaatgtt tctgaaggga ttgtatcttt cttagttaac
     1921 aatattgggg catttttagc tcctgacaat ccacttgctg acaatccatc agataatcca
     1981 ttttcagaat taataagtat tgcaatatca taattttgtt tatctgctat taaacctgct
     2041 gtttcatact tatttgtacc ttttattgaa ttaatagaac taagagcgtt tgatgttgga
     2101 caactagcaa tcaataatga aacagacatc cctattgcca atagtgtttt gtttaatttc
     2161 ataatgaaat cctcctataa attttataat attacttaaa tattataatt ctacataaaa
     2221 gaatattttc ctttatgaaa ttagtttata tttcaaaatt ttgtcacttt ttaaatctat
     2281 atactaaaaa atagactatt tgtaacagtc tatttcttag tatatcaaat atttatttag
     2341 gtttacgttt ctttgtacta gtttttgtat atgatattcc agttccaggt atactcaatg
     2401 tagctgattt tcttcctttt gaattaactg acactctagc acctttaaca ccagcactta
     2461 tacctacact cttcttacct aaatttaatt tgagtcctcc tcctagattt atgcttttcc
     2521 taaagttaaa tcccatagat ataaccttcc cttctatatt ttaaatattt atacttaaaa
     2581 ttatatttgt tatacacttg ccatcattgg tatatctagc atctatatct ttttacgttt
     2641 tttcttctta tatactactc cagcaactac agctgctgtt ccagctccaa ccccataagt
     2701 agcatttcta cttttctctt cttctctttt ttctaaacct tcatcatata caccttttaa
     2761 cttagtttca atagacttca tttcatctgg tatactaaaa tcttcttcat tgtaaccttg
     2821 attatataca ctttcttttg ctttaggtaa cttaacctta ccattatcat atccttcttt
     2881 aaaccaatta gcatatttaa cattacctat agttgatgtg tcaaatcttt ctcctttgat
     2941 agcactctca aatccttgag tagtgtaatc attttttaat tgttcttgta tttcgtcata
     3001 agcattatta taagcatcta taaatttaga atctatgttt tcttcaaatg tcattttttc
     3061 tttatcttta ttgctatcct ctatcccttt agctttatat tcttctatct ttttagtctt
     3121 atattcattg tatccagttg aatatccggt atcataagca ttttttaaaa cttctttttc
     3181 atatgtatta ttagatttag cacctgtaag ccctagatta tatccagtct ctttagcctc
     3241 cttttcttcc tcttctaatt tagccttacc tttttcatat ccttctgagt actttgattc
     3301 atatgtatca ctataatctc cactataatt tgatgaacta taatttcctt tatacccatc
     3361 ttcataccct tgattatatc ctttttctcc aacagacctc ttttgctttt cttcattatt
     3421 aaccgaagtt gctgttccag aactgcttcc tcctccagta taaggacaac caccattatt
     3481 atgtaaatga gctggataac caccacaatg atagtgataa cttcctaatc cagatttatt
     3541 tttattatca cgatgacctc ctgatgaatc agttcttcct gaatgagcat acgaaaagct
     3601 ccctacacta atcatagtta tactcattgc aacagctatt aactttttct taaacataat
     3661 ctatttcccc ctaaaatatt ctatgatttt attatatcag tcaatatatt atgtttaaac
     3721 cgttaaaatt cgacaatgta aatatttgat gaattgtatt tgtaaataaa tcttttagca
     3781 tatcttgaat atctgtagtt gttttgacat catattcttc aataagtttt ttaatatttc
     3841 gtttttgtta aacataaaaa gcgtgcactt ttctttgtat agtgatattt gatattttaa
     3901 ttatcactac tttagaagaa tacacacttt attttaaaaa ccctgatact atgttattag
     3961 aaaatggtac taatataaaa gatatccagc atatactcgg acatgctaaa acttcaacaa
     4021 ctatggatac atatttacat gtaactaaca acatgagaaa ccagacggta aatatttttg
     4081 aaatcattgt agaataaatt tgccacccaa aaataattat ggtggcaaat aggtggcaaa
     4141 tctactttta agacacacta taaaagtctg aaaccctgtt attctctaat aagtttaact
     4201 acagtctcac aatgtggcgt atttgggaac atatccatac acttaaccat cttaatctca
     4261 taaccatacc ccttaaaatc aaccaaatca acaaccaaag acttaggatt acaagaaata
     4321 taaacaatct ccttagcacc aaatccacaa atatccctaa tagcatcctt atgaatgcct
     4381 ggtctaggag ggtcaacaat aatcaaatca ggcttgtcct ttaaatcctt aacaacctta
     4441 gccacatccc cagcaataaa ctcacaatta gtaagtccat tcaacttagc attctcatta
     4501 gcagcaacaa cagcttcctc aactatctca ataccataca ctttcttagc agcaccagcc
     4561 ataacctgtc caatagaccc agtaccacta tacaagtcaa atacaacttt atcattgtaa
     4621 tctccaataa aatccctagc aatactataa agaacctcag ctcccttagt attggtttgg
     4681 aaaaacgaaa atggtgaaat cttaaactta agacctaaaa tttcttcttg tatataatct
     4741 cttccataca atactcttaa ctcatcacat tggactgcat ctgctaaacc atcattaata
     4801 gtatgaagta cacctactaa ctcagctttt aaatcaatcc ctaaaagcat atctttaaac
     4861 tcatacatat caaaatcttc ttgagaagaa gttactatat taactataat ctcattagtg
     4921 tgaatccctt ttctaactac aagatgtctc aaatatcctt tgtgattcat tgttctataa
     4981 taaggaagct ttttttcatt gaaaaactct acactagaaa ctaatatctt tgagaagtct
     5041 tcatcaacca acatacactc ctcaacagtt tgtatatcta tatgctttcc ttttttatga
     5101 agacctaatg tcaaaggtcc attttttact tcatctccaa aagtatattc catcttattt
     5161 ctataatatt tgttttctgg acttccttct atccctaaaa actgaaatcc aaataaatct
     5221 tgctttaaaa ataaatccat aacttgtttt tcttttattt ctaattgttt ttcatatggc
     5281 acagacaata gtgtacatcc tccacactct ctaaagtgct tgcaagtttc ttcagtttca
     5341 agtggtgagt tctctaataa ttctatcatc ttaacttcag ctttcttact tctaactttt
     5401 ttaactccag ctctcacctt ttgaccactt attccaccct tcatatggat aactctatct
     5461 ccaacttggc ttaaagatgt accaccaaat tccattttat ctacttcaaa ttctataaca
     5521 tcttttttct tcacagacat tcctccaatt tactatagta tctttattat ctttcttcaa
     5581 tcaattttac ttcttcatct gttaactctc tatactcacc taactctaaa ctttcatcca
     5641 attttaaatt gcccatagac aatcttttta agtatactac ttttttgcct acgctttcaa
     5701 acattctttt tacttggtga aacttacctt catgtattgt aagctctatt tctgattcat
     5761 catcactttt taatatattt aactgtgatg gcatagtttt atatccgtca tcaagcacta
     5821 caccttctaa aaaagcttct acatcttctt cagtaactac tccatcaatt tttgcataat
     5881 aagtcttagg cacatgcttc ttaggagata aaactctatg agaaagcttt ccatcattag
     5941 ttaaaactaa taatccctct gtgtctttat ctaaccttcc aacaggaaat ggttcaaaag
     6001 ctaagtatga taaatcaatt aaatctaata ctgttgggtc atatttatct gtagttgctg
     6061 atatatatcc atcaggttta ttcatcatca aatatatata ttctctatat ataacttctt
     6121 ctccattaaa taatatctca tcattttcag tgtctacttg agttcctgga tttgaaactt
     6181 cacttccatt tactactaca gacccttgtt tacaatactt ctttatttca cttctgcttc
     6241 cataacctaa atttgaaaga attttatcta tacgttgctt tttagccatt aaaaatgctc
     6301 tccattctca taatattttt ctttttcaaa tactgtaaat gctaaatcca tactttctac
     6361 atctaaatta tttcttatac tatcagttat tattcttgct actatatctt gtacttcttg
     6421 tcctctatca aaccaagcta cttcaacaaa aggatataca tctgctattt taccatctct
     6481 tattgcaact gactttatac attctatttc aaagtagtct cttggacatt ttacagcttc
     6541 aactaaatca tttatcattt tttcacttat tttacatata tcattttcat taattcctct
     6601 tatttttatc tgtggcatag tttatctcct ttattttaaa tttttagtga attatcatcc
     6661 acttaattcc cttcgataac gtttgaagtg gtaacttctt ggtcaagaca actattgtta
     6721 tcagtatctc caaactaata aggtagttcc tacccttata gttagcatta tatagctaat
     6781 tttcctaaat tgtttatatt ctttattatt ttatttaatc ctattgtctt ctatttaaca
     6841 ttatacttgt aatttatagt tattacaatt tactatatac tttttttgat ttaacgttat
     6901 aatataagtg atgggtaaat tttaaaagtt aataggagga gtttaaaatg gcattaatta
     6961 ctacaaaaga aatgtttaaa aaagcttacg aaggtggatt tgctataggt gcatttaaca
     7021 taagtgactt agaacaatta caaggtgttt taaaagctgc taaagctaaa aattcttatg
     7081 ttatgataca agcttctatg tcagctgtta aatatgctgg acctcataca ttagttgaaa
     7141 tggttaaggc tgcttctgat gaaataggtg tagatgtagc tcttcactta gaccatggtc
     7201 caaatatgga tgctattaaa acttgtattg atgctggttt ctcttcagtt atgatagatg
     7261 gttctcattt tgactttgaa gaaaatgtta gaataactaa agaagctgtt gaatatgcac
     7321 actctaaagg tgttgttgtt gaagcagaac ttggtgttct agctggaaca gaagatgatg
     7381 ttacttctga tgttcacaag tatactcaac cagctgaggc agttgagttc gttgaaagaa
     7441 caggtgttga ttcattagca atagctattg gaacttctca tggtgctttc aaattcaaag
     7501 gagaagctaa attaagattc gatatactag aagaaatcca aagcaaatta ccaggtttcc
     7561 caatagttct tcatggtgca tctgctgttg accaaaacgc tgttgctaca tgtaatgaat
     7621 ttggtggaaa tatagctggt gctaaaggtg tacctgtaga tatgttaaga aaagcatctt
     7681 ctatggctgt ttgtaaaata aacatggata ctgacttaag actagctatg actgctgcta
     7741 tcagaaaatt cttagctgaa aatccaaaag aatttgaccc aagaaaatac attggtgctg
     7801 gtagagatgc tatacaagct gtagttgaaa gcaagataga tgatgtttta ggttctgcta
     7861 actctataaa ctaatataac ttttaaatat ttataaaaga aaagagatga cttaaagtaa
     7921 ttaaatttat ttttaagcca tctcttttaa gttgtataaa ttatgtatct tataatattt
     7981 taattatatt gtctttttaa actgacagct aaacgtgaat atgtccactc tttatctttc
     8041 tatggatggt agaaggatat atacctataa cttcagcagc ctttgttatt gaaccatact
     8101 tatttatagc cttacttatc attatttggt ccactatttt atatgcatca ttaagattca
     8161 taattccatt taccactatt ggtgataaat catctgtttc attatcaatc atatctaaat
     8221 ttatcagcat gttaaattca tcttctccaa ttacattttt agctgataat actacaaatc
     8281 tttcaatgat gttttttaat tctcttatgt ttcctggcca agaatattct tctaataact
     8341 caataacttt aggcgaaact tctacttctc tattgtactt ttcattgtaa agttttaaga
     8401 aataatttac taatggtact atatcctctt ttctttctct aagaggaggt atttttactg
     8461 gaataacact taatctatag tacaaatctt gtctaaattt aaggttattt ctaaggtctt
     8521 catgagaaat attagtagca cttatatatc taacatctat ttttataggc ttactaccac
     8581 ctattctagt aatagtattt tcttgtataa ccctaagtaa ttttttttgc atctgcattg
     8641 gtaactcacc tatctcatcc aagaaaatag ttccaccatt cgcctcttca aaaagtccct
     8701 ttttaccttt tttcttagct ccagtaaaag aaccttcttc atatccaaaa aactctgact
     8761 ctaataattc atttggtata gctccacagt ttatagctat aaagggtttg tctctccttg
     8821 aactatactt atatattgtt cttgctatta tttcttttcc tacacctgat tctccattta
     8881 taaatataga agaatctgag gctgcagctc tcttaacaac tttttttata ttgtccataa
     8941 tcttactttg acttatatac tctgtgtctt ctgatatatc actatatttt atctgagatt
     9001 caagcttaat aacccttgaa atatctctcc ctgttagtac tcctcctgta agctttccct
     9061 tagaatccca tcttggaact gccgtatatg caataagctt cccatctggg taaacgatat
     9121 ttctataaat ctttttcttc actttaagca tgtttaatat tattggttct tcaactaatc
     9181 cttgtctcac aaggtctgtt atatttctac caagtacctc agttctttta aaaggtaata
     9241 tctcatcact gacagtgttc atcttttcaa ttaccccatc tttgttaaac acaacaacaa
     9301 cttcatctat atgctctatt atatcttcaa ttactttatc agatattaga gacttctcaa
     9361 ataaataaac tatattattt tcattattat tatctttcat attgtatata tgtgcagtca
     9421 tccttaactc tctgaaagct attttctttt tactatctgt cagtatttct gttttgtcaa
     9481 aatttgaatc taattcattt atatttctag gcttttttat atctagaaaa ctaaaaatac
     9541 tatctattag atgattacag tatattattt cgcctgattc tttacataaa atggctggat
     9601 tttctacatg ttctataagc cctattaaat tattatccat taatattcgc acctttcatt
     9661 aagtaattta ttgaactaca tacaaaattt attgtttgct tacaataaat accatcagca
     9721 catataatca actccttata atattcgtct tttcaagtag tttatttcat taaaacgtaa
     9781 ctaagatata attttaatga ttatagtttg ttaaaaattt cattttgctt ttaattgtat
     9841 tttatcacat atttagtaat tatttgtcta tttaatacaa tttaattaaa tttaagattt
     9901 atttaaaaaa acaaagataa tccttacaga atataactca caatttgtaa gcaatataca
     9961 tataaggatt atccagatta tcttgtatta taaaatttta ttcaaacaga ctttttaata
    10021 actgacaaat acacactagc aataacttat caaagggtta ataattatgt tactaactaa
    10081 taagacttat tcaatcagta atatttatat agtttgtatc tattaagctt ttatagcttc
    10141 taataaatca tcaacgctat ctaatttttc tataagcatt ggaactattt catgtaagtc
    10201 tccaactact ccatagtcag cgatttctaa aattggagct gctgggtttt tgtttatagc
    10261 aattatgaaa tctgatgatt gcatacctgc taaatgttgt attgctcctg atataccaca
    10321 agctatatat aggtttggtt taacagtagt tcctgtttga cctacttggt gagaatggtc
    10381 tatccatcca gcatcaacag ccgcacgaga agaaccaact actccaccta atttgtcagc
    10441 taatttctta agcatttcaa atccttctgg tccacctaat cctaaaccac ctgatactat
    10501 aacatttgca tctgtaagag atactaaatc tttcttagtt ttaactgttt ctaaaacagt
    10561 agttcttata tcatcttgag ttattttgta gtctaatgct ataacttcac cagttcttgt
    10621 ttcgtctttt tcagctttat ccataacacc aggtctaact gtagacattt gaggtctatg
    10681 gtttggacaa atgattgtag ccattatgtt tccaccaaat gctggacgag tttgttttat
    10741 tttcttatct tctgggtcta tttctaactt agtacagtca gctgttaatc cagttccaac
    10801 tcttgaagct attctaggtg ctaagtctct acctatatga gttgctccga aaagcattat
    10861 ttctggtttt atttcatcta ttgcatcttt tattactttt gtatatgcat cagttgtata
    10921 tttttctaaa agagcatcat ctgcaacata aactttgtca gctccaaaag tgattaattc
    10981 tttagctaat ccatcaacat cttttcctaa taatattgca caaagttctg catctacttc
    11041 tttagctaat tttcttcctt ctcctaataa ttctataact actggagcta tttttccttc
    11101 tctttgttct gcaaatatcc atacgttttt ataagaactt aaatctttta tatcattcat
    11161 agtttgtact cctccaactt atataatgtg cttttgtttt agaccgatta ttattttact
    11221 agctatttca tcagctttac cttctaagat ttctcctttt cctcttggtt caggagtaaa
    11281 tgatctaaat actttagttg gtgatgcttt taaaccaact tcttctttat ttacatctaa
    11341 atcatcaatt gtccaaactt ttacatcatg tttatatgct tttactattt tatcaacaga
    11401 catgtatcta ggtgtattta actcttttac tgctgttaaa agtacaggtg tacttacttt
    11461 tattaattcg tatccatctt ctaattgtct ttgaactatt atatcttttc cttctatttt
    11521 gaaatcttgt acatatgtaa cttgaggaat gtctaatttc tctgcaattt gtggtccaac
    11581 ttgtgcagta tctccatcta ttgcttgtct tcctgcaaat attatgtcat agtcacctac
    11641 tttagatatt cccgctgcaa tagtatttga tgtagcccaa gtatcagctc ctccaaatgc
    11701 tctatcactt aataatattg catcatcagc acccatagct aaacactctc ttagcataaa
    11761 gtctgcttga ggaggtccca ttgttataac tgttatagtt acatcatcat gctcatcttt
    11821 aatttttaaa gcttcctcta atgcatttgc atcatctggg tttaatatac ttggaactcc
    11881 atctcttata agagtacctg tttctttatt tattcttact tcgttagtat ctggaacttg
    11941 ttttacgcaa actaatattt tcaaagtcta tacctccaat ttcattagat tatttattat
    12001 tttaatacgt ttgatgaaat taccattttt tgaacttctg atgttccttc gtatattgat
    12061 acgattctag catctctgta cattctttcg atttcataat cttttataaa tccatatcca
    12121 ccgtgaagtt gtaatgcttt ataagcaact tcatttgcaa tttctgatgc atagtattta
    12181 gccatagcag attcttgagc aactctcatt cctgcatctc tcttttgtgc tgcatcataa
    12241 actaatcctc tagctgcaca aacttttgtt tccatgtcag ctatagtaaa ttgagtattt
    12301 tggaatttag ctataggctt accaaattga actctttgtt ttgtatattt aacagcttca
    12361 tctaaagcac cttgagctat tcctagagct aaggcagcaa cacctattct accaacttct
    12421 agagtaccca ttgctatctt aaatccttga ccttcttttc caagtaagtt ttcttttgga
    12481 actttaacgt tttcaaatat taagtcagaa gtttcagttc ctcttatacc catcttatct
    12541 tcatgagctc ctgttgagaa accatcccat ttactttcta ctataaacgc tgatgttcct
    12601 ctaagtcctt tacttctatc tgtaactgct attactatag ctacatcaca gaatggtcca
    12661 ttagttatga aagtttttct accatttaat atatagtggt ctccaactaa ttctgctgtt
    12721 gtttgttgtc cacctgcatc agaaccagct cctggctcag ttaaaccaaa tgctcctatt
    12781 aattcacctg ttgctatacc ttttaagtat ttttgttttt gctcttcagt accattgtaa
    12841 agtattggta atgaagcaag agaagaacca gctgtaacaa aagtagctgt tgaagcacat
    12901 cttctagcta tttcttccat tactatagcg taagaaacat atcctgctcc cccaccacca
    12961 tattcttctg ggatattact gcttataaat tttgcatcag ctaatttttt gattaattcc
    13021 ttaggcatta cacctgtttt atccatttct gctggtaaag tatctaattc tttactaaca
    13081 aaatctctta ctgcttttct taaaagttct tgttctttgt tatataacat agcttcctcc
    13141 aattttcata ttttatagta aatttatttt aatgaactta cgctaaactc atcatctcag
    13201 caaaagtttg aatacgagtt ctggcttgtt cattattctg agtttgttgg tcgatttcaa
    13261 catataaagt aggtattcca ctatcttcta tatcttttct aactaaagga taatcgtatt
    13321 cttctgggtc acagaatttc atcatacaga agataacacc atctatatct ttctttttaa
    13381 cttcatctac tataagtgac ccacgttttt tcttagggtc ataagctaat gaacatcctt
    13441 ctatgtttga ccattgtctt gctaatctct ctaacgcatc atcacctgct ggtacatctg
    13501 ttctgaattg tcttgtttct tgtgctaagt cgtcagctac aactgatata ttgttgtctt
    13561 ctaaaatgtc taatatatct ttagaatcag ctaatatacc tgttaataaa actttctttc
    13621 cagaacagac ttcttctggc atagcattta attttgctat taaatctttc actagctcag
    13681 tatgttcttc ttttctcata aagaacccac tcttaattac taagcttctt attgatggtt
    13741 ttatagtatt agaatgttta taagctactt caacaaagtc tctcatagtt tttctatgtt
    13801 cattgtaaac ttctatactt tcatgaattt ttgcttcttc tatttcatat ccacaaattt
    13861 cttcaagttc tctttttaca cctttgtact cactgattaa gtattttaca ccagcttcaa
    13921 gttttctatt ttgtgggtgt actaatgata tatattttat atgaggtact gctgatttcc
    13981 agttttgtcc taaacaaatt agtgtatcac acatacctgg tataataact ccagataatt
    14041 catcataagc accttttaat ccatattcta aacatgattg cattattgaa catgcaaatg
    14101 cagggaaata ttgtttagct aaatctaatt ctgtttgtcc tccccatata ccaactggaa
    14161 gcattccagc tgcatgtata atttcttctg ggcaataaac tgggaaacaa cctatagctt
    14221 ttttaccagt ttcactttta tattttttta cagccgcatt tggattttca actacttctt
    14281 tcattttaga taaaatagct tccattatat ctcacctcta ttaagttttt ttctttcttc
    14341 cattacttca actaaacctt gaattcttgt ttcaaattga gcattagtga aagctctagg
    14401 gtctgcttgg tcaccatcaa atccagcata tggtaatcca gtttcttcag ctgctcttct
    14461 ttgcatttca tattgtatta aactcataag tttacagctt ctgttcatat gatagaatgc
    14521 tccatcacat ttaccctcta ctaaagaatc aactctatat tttgtcatac ggtctaggtt
    14581 tacattgtta aacatagtac tatatgctac tgccattcca tctaaatcat taacttcata
    14641 ttgtaatgcc caagcatgtg ggtaaacact acctgtcatg ttaactccaa atttagctaa
    14701 tgttttcatc ttgtatccta tatatggcca acaaggtata ccttccatca ttattctgta
    14761 tttttcttcc cctctgaaag aagatttacc agttttcatg ttgtcctcta attcttctat
    14821 aagtaactta aatgcttctg tagtttcttt tttacctcta gcacaaacta ttacagccat
    14881 gtaagtaaat aagtcaaatc cattcattgg agaaggagaa gaatctgctg gtaaactcat
    14941 agaatacttc catagtcttc cgttttcagc tgatattttc attacttctt caaacttctt
    15001 agggtcaaat ttctttcctg atataatttc tagttgtttt atagcttctt caaattgagc
    15061 tttaatataa tctattcttg attgagtaac ttcgtcttca ttattgaaag ttgtatcaat
    15121 cattattaaa ggtatatcta attctcttga aatattttca taccatttta taacttggtt
    15181 acatatattg ttacagcaaa gtaggaaatc tggagccggc atatccaaag cttcacatcc
    15241 accattttct aaaagaccaa aatttgttct tgcatatgca cataggtcaa tagaatatcc
    15301 tttagattca gctatttcac ataattctaa agaacctttt ttagctgcta ctccagctgc
    15361 ttggttttct ggatataata cgtttaagtc aaatacttct gctaactctt gaggaaatac
    15421 tgatgttgac caacctacag gtcttccttc ttctttagct ttaaatgcat ttgcatattg
    15481 ttcagctaat aaatcattaa ttactactct agcttctttt ttttcagaca ttttcatccc
    15541 tctttttata tatttttcac ttctttttgt gattcttttg cttcatcaaa agcatataac
    15601 gctgctccta aagcaccagt taattgaggt atatcaggta caataatttc tgtgttgatt
    15661 tctcttgcca tagctcttac aataccacta tttctagcaa ctccaccaac cataactaca
    15721 tttctttgta ctcctattct ttttactagg ctagaaactc tctttgctac tgaagtatga
    15781 atacctgcca ctatatcttc aatttttgca ttttcagata aatgtgatat aacctcagac
    15841 tctgcaaata ctgtacatgt actgcttatt gatacttcat tttgagaatt catagatata
    15901 cttccgagtt cagatacatc aacctctatt atttttgcca ttacatctaa aaatcttcct
    15961 gtacctgcag cacatttgtc attcataaga aagtttaata gtcttccatt attatctaat
    16021 tttaatacct ttgcatcttg accacctatg tcaataatgg ttcttgtctc tggaattatg
    16081 aaatttaccc ctctagcatg acagcttaat tcacttattt gcttatcagc atctgaatag
    16141 ttcattcttc catatcctgt aactacaact tttttaatat cttctcttgc aagacctgtc
    16201 ttgccatata atttttctaa aactcttgat ggtccagtag tcccagtacc agaggatatt
    16261 gtttcagaag ctacaatatc ttccccattc tttaagatta ctccctttga tgcagttgaa
    16321 cctatatcta atcccattgt gtacatcttt agcattctcc ttttaaaaaa tctagaatta
    16381 ctaattaata tcttacaact ttactatctt taaagttatt tattttctct tcatcgtagc
    16441 ctaaagattt taatacttct acagtatgtt gacctacttt tggtgctggt gtatattctt
    16501 taattccttc atttctaaac ataactggag tattaactaa gacacctgta tttccgctat
    16561 cgtatgtttt cttaaataag aagtcatttg cccaagcttg ttcgtcatct aataaatctt
    16621 cacagctttg aattttttca aatggtaagt ctgcttcttc taataaagct gaccactcgt
    16681 ctaatgtttt ttctaacata gcttctccaa ctatcttaac taaatcttca acatgattaa
    16741 ccattgaatc tatgttatta tatctatcgt cttctaatat atattctcta tttataacct
    16801 tacagaattt gcctaaccac ttgttgtatt gtattaaagc taattgaatc catcttccat
    16861 ctttacattt atatgtagtc attaatgggc tgtttggatt ttctcttgat aaaggcattt
    16921 catttccgta ttgtgctgtt gttatcattg ttcccattcc atatatagct gtatggaaaa
    16981 gacttactgt tactctctca cctttaccag tttgagcttt tttatgtaat gctgctaaac
    17041 ttcctgctgc tagtgctaga cctgcatagt ggtcaccaaa tcctgctgct gtatttgctg
    17101 gagatgttcc tttttccata acagattggc taactcctcc tcttgcgaag tatgcagtat
    17161 agtcaaatcc tggtttatct tttaaaggtc ctttttcacc atatcctaat atttgagaga
    17221 atattaatcc tggatactta tcttttattt ggtcataagc tatacccatt ttttctaatg
    17281 cttgaactct aacattagtt acaaatatgt ctgcttctga taataattta tgtaatattt
    17341 ctactccttc ttttgatttt acattaatac ttacaccctt tttatttcca ttttctaatt
    17401 caaacatagg gttttcatca tctgatgctg gagatttaaa tgttccaccc ataactctta
    17461 ttccatcacc ttctataggt tcaatcttaa taacctctgc accccagtca cctaacattt
    17521 ttgcacaaca tggtgctgcg atgaaacttg aaagttctac tactttaact ccttctaaaa
    17581 gcataacgtt ttcctcctta atagtatcaa ttataaaatt tcgattaaat cactcataaa
    17641 atactttttt ctgatattaa ctgtctgatg tcaaagttcc taatcttatc taaaataact
    17701 taaaaattat taaaattatt ttttattgtt ttattgtctt acacctatat tgttaagcat
    17761 ttatcgtgcc aactttatat ttaatcaaaa tatcgcttga tttgaatgtt ttttaacaat
    17821 atgcaattga ataatctttt tcatatcgtg gcatcttgcc actatccatt gcattttgct
    17881 attctttcta tgcttataaa agtttttttt atatattttt ttatgaaaaa gcttgacatt
    17941 tcaaaaaaag ttttcattgc taaactcaaa aacacatgtt atcattgtaa ttaatcagca
    18001 agttagttaa ctaatttcaa acttagataa ttttttaata atatttaatc taaaaaataa
    18061 caatttcttt gataaatttt agagtaatta ttacattaaa taaaaaatta ctaaatatca
    18121 aaataacaca gggaaaacaa tttcaaaact aaatttttaa ccttaaaaat tttgtataaa
    18181 aaatataacc ttatatgcta catattattt ctaaatgaaa aattaccata ataatttaat
    18241 aattacagga gggtattgat atgaaaatac tagtatttgg agcacgcgat tatgaagaac
    18301 cagtaataaa aaaatggtct gaagaacata aggatgttca agtggatatt tatcctgaaa
    18361 acatgactga agaaaatgta gttaaagcta aagggtatga tggtatatct atacaacaaa
    18421 ctaactatat agataatcct tatatttatg aaactttaaa agatgctggg gttaaagtta
    18481 tagcttcaag aactgcaggg gttgacatga tacattttga tttagttaat gaaaatggac
    18541 ttatcgttac aaacgttcct tcttattcac ctaatgcaat agctgaatta gctgttactc
    18601 aagctatgaa ccttttaaga aagactcctc tagtaaagaa aaaagtctgt gaaggtgatt
    18661 accgttggat agctgaactt cttggaacag aagttagatc tattacagtt ggtgttatag
    18721 gtacaggaaa aataggtgct acttctgcaa aattattcaa aggcctagga gctaatgtaa
    18781 ttgcatttga ccaatatcca aatagtgatt taaatgatat attaacttac aaagattctt
    18841 tagaagacct tctaaaagaa gctgacctta taacattaca tactccttta cttgaaggaa
    18901 caaaacatat gataaataaa gatactctag ctataatgaa ggatggagct tacatagtaa
    18961 atactggccg tggtggttta attaatacag gggatttaat agaagcacta gagtcaggaa
    19021 aaattagagc tgctgccctt gatacatttg aaactgaagg attgttctta aacaaaaaaa
    19081 tgaatcctgg agaattaact gacccagaaa taaataaact tctttctatg gaacaagtta
    19141 tattcactca tcaccttggt ttcttcacta gtacagcgat tgaaaatata gtttattcta
    19201 gtttaagtag tgctgtagaa gttataaaaa caggaactgc tactaataga gtaaattagt
    19261 attttataaa tatatttttt cattaattag ctattatcta acaaaatagc ctttaatttc
    19321 ccaaatataa gagagtcagc tcagtatata ttgagtagac tctcttatat tattttttat
    19381 attaaacttg tactcttctt attcatacca aattaaattt atattaactt actctaacta
    19441 ctcctactct atacatttta cctattttta cttttctaat tatatacatt tgaatgggta
    19501 caatctgtat tttacaaaaa aaacacctaa caaactatga aaattgtatg attaatatta
    19561 atatatttat aagaaaacat aacaattatg ttagctctgg atttttaggg agggagtata
    19621 ttggaaaaaa tgaataaagt tacttctaaa aaaactacct atattgttac ttcagcatta
    19681 tttgcatcca taatatgcct gacaatagca tatatattac atattccagt tggtggaaac
    19741 aatggatatg ttcatatagg agatgctttt atttatctag ctgcaactat tttgccaaca
    19801 aactatgcta tagctgcttc tgcaattggt gctggtctag ctgatttatc tacaggtgct
    19861 gctatctggg taataccaac tataatcata aaaccaatac ttgtattatt tttcacttct
    19921 aaaagtgaca agataataaa taagagaaat atagttgctt cggttgtagc aggtatagta
    19981 ggattagttt tatacatgtt tgctgaaggt ataattattg gaagttttac aagtgcattt
    20041 gtaatgagct tacttggctt attacagcca ataggaagtt ttattgtttt tataatctta
    20101 ggtatggcac ttgataaact tgattttaaa aaacgttatt ttaattaaga atttaaattt
    20161 atggagggaa aattatgaat atcttatata caattgaaaa tagtatctat gtaaatataa
    20221 ctaatacatg tccatgtagt tgtgtttttt gtataagaaa cgaaaaagat gaagttgcaa
    20281 atagtggtag tttatggttg gagcatgagc caagtgtaga tgaagtaaaa gaagccttca
    20341 ataaatacaa tttagacgac tatgatgaaa tcgtattttg tggatatggc gaaccattaa
    20401 tgagaataaa tgaattgata gaggtagcaa agtttataaa agaaaaaagt agcataaaga
    20461 taagaataaa tacaaatggt ctaagtgatt taatacataa caaaaaaaca gctattctgt
    20521 taaaaaatgt tatagatgca gtatcaataa gtcttaatgc cccaaataaa gaagcttata
    20581 atagagttac tcaaccaaag tttggagaaa aatcatttga ttatatgtta gattttgcaa
    20641 gagattgcaa aaaatatata aaagaagtag ctttttcagt agtagatgaa atatctccag
    20701 aagaaataga agaatctaaa caactggcaa aaaaacttga tataccatta agggttagac
    20761 ataagaatta gcaagtaaaa ttataaaaca ttaagaatca cctgaatggt ctttcaggtg
    20821 attcttcctc ttgtattaac attttacaaa atagatttca taccacacta ggaatgtgta
    20881 aatcatccaa atctttctcc aattatcaga gtttccacta atgtagtcat taaaaatctc
    20941 attaatctca tctatgttaa agaatttatc tgctatctca ctattgaatt tatctcggat
    21001 atccttatta tattggtcat cagccatcca tatacgaatt ggcacaataa atcctaattt
    21061 atttctaaaa gcaatctctt ctggtaatgt ttttgatgca gctgtacgaa aggcaatttt
    21121 attctctttt cctttcactt tatattctga tggtattctg gatgcaatat caaatattct
    21181 tgtatctgta agaggcattc tgacttccaa tcctactgca gtactcattt tatctatatt
    21241 aaaataaata tctccttcca accatatttg aatatctaca tttgacatgt tagtaagtgt
    21301 atctagttct tctgtttctt cataaattcc ttttaccact tcaataggaa gcacatcagg
    21361 attgtatttc ttaagaattt ttcttttctc accctcattc ataatgtttg tatttccaat
    21421 ataaaatgtc ttcaaattct ctccataaca ctctgcctta tggtatatat tatatccacc
    21481 aaaaaactca tctgctcctt ctccagagta acagatattt gtgttttcag ctgctgcctt
    21541 gcattcaata gcaaaaacga tagcagatgc atctccagtt ggttgctcca tatggtacat
    21601 tacataaggt actgacgcaa aatactcttc aggtgtaatt atatatctcg aatttttaca
    21661 cccaagaata ttagctgtct gttgtgctaa ttttgattca tcaaaacgct cttcctcata
    21721 accacatgaa cctgtttttt ctatatctga cattgccaat atataagatg aatcaacacc
    21781 tcctgatagg aataactctg cagtttcatt ctctgatttt acctctggca taattttctg
    21841 aagtgttgta tgaatttcat ccgtccattc ctcaatagac ttgctgtggt caggatgaaa
    21901 ttcaggtttc caataacgac caatacatac tgttttattc tgatagacaa ggtaatgtcc
    21961 tggcataagc ttctttactc cacggaagaa tgtattttca cctgctacat atgtgaaaga
    22021 catataaatc tgtaacatgt cttcatttaa ctctttcaca aatcctggtt gctccataat
    22081 cttacgaata gatgaaccat agagaagctt cccattttca gtctcataat aatagaaagg
    22141 ttttacccca aactggtctc ttaggcagaa gagtttcttt tccacagtat cccataatgc
    22201 aaaagcaaac attccatgca tgtgatttgc catatcatat ccccatgtct tatatgcttc
    22261 cactaaaatt gctcgttcac gctcatctct acataatgaa gaatcgattc caagttcttc
    22321 acaaagaact ttccagtttc gaatatgccc attgtattct agaattttta tcatatatgt
    22381 gtcttttcgt gttaaatact tttcttctaa atttatcata tgtgtatcct ttcgtattaa
    22441 aacatttaac ttctaaattt attaaatgtt tatatatatt tattcactta taatcactga
    22501 aaaatcgact tttttcaaag taaattcttt ttgaattagt actaatctgc tctttattaa
    22561 tggcataaat atgattatca agtattatca gtgttataac attgaatttt ttattcttca
    22621 taaatattgc cccctttata ttaccaacta aatataatat atcttatgtt acttacatat
    22681 tctttaagaa attctgaaga ttttataaag ttttttgtat tataattaat taaaaataaa
    22741 attctagtta taaaacatat tttccattaa acaaaaaaat agttaaaata aattcataac
    22801 cacataaact tattttaact atttgtatat tttaattata aatattaact taaattttta
    22861 acaggataca ctcataaaat aaacataatg aacaacaaaa taatatgtct cagtttcttt
    22921 atatacttta ccttctttca attttctacc atgtagacgt ataagataat attagtaatt
    22981 cactctattg cttaactcgt gcgatatgga caaccaagaa agctatttcc tcatttgtta
    23041 acttttttcc atatttcttt tctaaaaaat ctctaacttt taatgcaatt aacatttctt
    23101 ttggatttga ccttttcatt tgattaaata ataaatcatc accactgctt aacatactat
    23161 ctgaaaaaaa tctttccaca aaaaatttta tatgtgttat aaatctcata taatgcatac
    23221 tctttttatc taaggttcta ttcaaagaaa aaacaaaaat attaattatt tctccaatta
    23281 ctttggcata cttaatcgta tcatatgaat ttccatctga agccatagca tttgcaaaat
    23341 ggaaagcaat gttagctgct tcttcctctg gtaattgaat gcccaatctc tcctctacca
    23401 tataaattgc aaccattcca accttaaact catttggata ataatttttt atttcccaaa
    23461 atactcgatt tgtaattttt attcctttac gcatcctttc aattgcaaag ttaaaatggt
    23521 ctgctaatat taagtataag tttttattaa aagatgagtt caataacttt tctgcctcta
    23581 ctattatttc ctgtgttatc tttaaaatgt cgacaggaat atcatccatt aattctaaaa
    23641 attgttttga ttttgtattt tcaacaggga caaacatttg attatcattg gaaccacaaa
    23701 tatattctcc tgttttttta ccatatccaa ttcctttccc tagcaaaata aattcattat
    23761 cctttgtgtc atgaactaaa acaacgctcg agttcaaaac tttctttatt atgtagttca
    23821 ttttatttgc tcaccttcta ttggtcattt aaaatctata ctattacttt caattacctt
    23881 tttataccaa taaaaactat ctttcttata tcgattatat gtcccctttc cataatcatc
    23941 agcatccaca taaatatatc cataacgttt tgacatttgt ttcgttgatt cactgaccaa
    24001 atcaatacaa ccccatgaag tatatcccca aagttcaaca ccatcttcaa taatcgcatc
    24061 taacatacat ttaaagtgtt cttttaaata attaattcta tattggtcat gaactttttt
    24121 atcttcagtt aaaatatctt tggctcccaa tccattttca actataaata aaggctttct
    24181 gtatctatca tataattcaa tcaaagatat acgtagacct attgaatcaa tttgccaacc
    24241 ccattcactc atttctaaat aaggattttt tacagctaat aacgtattcc caggtgtaat
    24301 atctaatcca gttgtatcag ctgcctcaca agaagacata tagtaagaga atgaaataaa
    24361 atctactgga tactttttca taatttcatc atcatgttct tcttttacta tttgaatgtt
    24421 attattttta aaatacgaca gtaaatatac tggatactca ccaaaaacct gcgtatcact
    24481 atatgcataa atacttctca tttttcgttg agttgccaaa acatcttctg gtctacatgt
    24541 gtatggataa tacgttaatt ttgttaacat acaccctact tttgaatttg gaataatttc
    24601 atgacaaatt tttgttgcaa gggcactagc tacaaattga tgatgcatag cttgaaaaat
    24661 aacttcctca aagtttttat ccttaaaacg gtctcgaact aaaccaccag tagtgtacgg
    24721 atgacgaatc atagaatcaa cttcattaaa tgtaagccaa tatttcactt tgtttttata
    24781 tctttcacag atagtttcta catacctaac aaacatattg ataacttgtc gtgaatacca
    24841 tccatcatag tttaaaacga tatttaatgg tggttcataa tgtgacatag taacaagtgg
    24901 ttcaattcca taacttatca actcatcaaa tacatcatca taaaactgta aaccttcttc
    24961 atttggctct ttatcatctc catttggaaa aatacgagac caagcaatac tcattcgata
    25021 tgtcttaaat cccatttctg ccattagagc aatatcttct ttatagtgat gataaaaatc
    25081 actaccatga cgttttggat aatttacttc atcttcagga tgtgctaatc cttcttctat
    25141 atcttttgtt gtgatttcat tattagattt ataatctttc acatctgcat caaagtgtgc
    25201 ctttgctaca tctgatacag accacccttt accacctaaa ttccaaccac cttcaaattg
    25261 attagcagct gtagctcctc cccaaagaaa tccttctgga aactttctca tatttatatt
    25321 cctcccttta catcaaacaa gatacttcct ttttttacat tgccataaga ctggtttaac
    25381 actttataag cataatctgc tccattattg tttacaatca taacagttga atctaaatta
    25441 gcatgtgaga ttttgtctaa atcaaactga attaataaat ctccttcttt tacattatcg
    25501 cctgtcttaa caaatgtttt aaatccatct ccattcatac ttacagtatt tattccaata
    25561 tgaatcaaaa tctcagcccc attttttgtt cttaaaccaa ttgcatgttt tgtttcaaat
    25621 aacatcacaa cttctgaatc acaaggtgcg tataaatttc cattggtcgg tataattgca
    25681 actccatctc ccaaagtctt acttgcaaat agttcatcat taaccttact taaatctatt
    25741 acttttcctt ctactggagt tactaagata tttacttctt tttcttcttc tagctcttct
    25801 tcaacaacat ctccaattga tgactctact gtatttgtat cttccttcca aataatcagt
    25861 gtaataataa acgagcctaa tattgcaata acaaaaccaa tacttgcaaa aatcaaattg
    25921 tttccattgt ttgggtcaat aaacatactc atactggcaa gccctggtcc aacaattaca
    25981 aatcctttta ctgcaaataa tcctaaaaat gtaccagaaa ttgcacttga gaatacaaca
    26041 cctaacattg ctcttttatg ttgtaaagta ataccatata aagctggctc tgtaattccc
    26101 atcaaggcag aaattcctgc agataatgct gtttgtttta atgtttcatc ttttgttcgc
    26161 aaggctactg caaaacaagc accactttca gaaatattgt gggctaatga agatgtcata
    26221 tataaagctt cataacccaa ctttccaatt gtattaattg aataaggtat tagtgctttg
    26281 tgcattcctg ttgcaaccat aaatggaaga aaagcagcaa gtaatcctac aactaagaat
    26341 cccattttgt tatataagaa taaaataatg gttgtaaaaa ttgtaccaac gttatatcct
    26401 aaaggaccta aaatagttag agtaattgga acagtaattg ccaaagacat cataggaaca
    26461 aagaaagttc gaattggttt tggtgaatac ttgttaaaaa ttttctccat tgttgccaag
    26521 aaagacacac ataagatagc cgggaaaacc tgtgcattat aggcaatgtt tggaattgta
    26581 aatccaaata gttcgacacc ttcacttaaa gcagttgttg ttgctggaag caataagtat
    26641 cctacagcgg caatagctac taaacgatta catttcaata cattggcagt agttacagct
    26701 accataagag gtaggaaata gaatgttgca tctgaaatag caactaataa tgtatatata
    26761 gtagaatctg attttaaaat attaaacatt gctaataaca tcaacagtga ttttaaaata
    26821 ccagctcctg caatagcaaa aatcagtggt tgaaaaatat taataataaa ttctataaat
    26881 cttgttccta agtttatttt ttcttcagtt tttacagtac tatcatttgt atctaaaggg
    26941 caaactttta aaatttcatc atacacatca acaacactat ttccaatgac aacttgaaat
    27001 tgtgccccat taactatact cataacacct tgtacttttt ttaaagcatc ctgattgact
    27061 ttacttctat caatcaactt aaagcgtagt ctagttgaac agtgttctaa agctaaaata
    27121 ttttttgaac caccaacttg ttcaataatg tcaatggcag tttttttata attcatcttg
    27181 taatctcctt ctccataatt ttttatgaat ggccattcaa gatgcaaaaa aggcagaacc
    27241 tatataaaca ccttgaaaaa tcaaagcaat atattcgatc ctgcctgcat taccagtaac
    27301 atgtcttcta tttatactat tttgtattat tatattaaca tttgcctaaa agaattgtca
    27361 atactttttt aaattttctg atactaacta atagcacctt tataattcat tttttatatc
    27421 aattaaagta acagtagcca cccaccaatt agtatcaaaa ttgaacctgg tattttttct
    27481 tttatatttt ctttgaatat aaaatgagca agtattaaag ttattatcat agatacttga
    27541 gaaaatggct ctgctatact aagttctaga ttatttaaag caattaataa tgctaaataa
    27601 gaatatccat taatagcacc acttaaaatt gaaagcccct ttttattact aaaaatctca
    27661 caaattattt tttgtttctt tttaaatgct aaaaagataa ataaatttaa acctacgaaa
    27721 aaatacagta tagttgaata taatatagga gatacttgta caaaaaaagc cttatccaat
    27781 actcttccta ctgactgcaa aaatatgtat aaaaacatta atctacatgg taaattgtta
    27841 aaaattacag agacattctt taaaaaagag cttacttttt ttaatataaa cactccacca
    27901 atcataatta tgattcctgc aacttttgct aaacttaaac cttcacctaa aaatataact
    27961 gataaaagca gtaaaaacaa tgaatttaaa ctatttattg gagtaacaag tgaaacttcc
    28021 ccagttgcta aagacgttac atacgctact gaaccaattg aatagactaa actacttata
    28081 taacatggta ttaaaaacga ccaatcacta actctatcca ttatgctaaa tggaagtaaa
    28141 catactcccc ctataaagaa aaatagaaat gatgaagcta tatttttata aatatctcct
    28201 tcttcatcac ctaataatct tacaactact ctttcaaaag ctaatagcac tactctaatt
    28261 aataatgcaa aataagataa tatcattttt gacctcgctt aaaaattaat ccgacttatt
    28321 gttatattaa ctgtataaaa ctaccatata ggtaagtctt ttggattttt taatatttta
    28381 tgtttaaata caggtacatt aagtttttca cctagtttac gacattgctc tttataggta
    28441 ccttttaatt tatcatgaaa aactatacaa tcaattcctc cttcaatact atttatacac
    28501 ttttctagtt tctcaaatcc agattcatca tcatacattc cacttacaat ccattgattt
    28561 ggcaatacat gtccaattga tatgttatat tctttatttt ttataatatc tgagaaagat
    28621 ttctcaaaat gtacccctcc aacacataac aaagtcttca aaggttcatc acaatcaaat
    28681 actctaatta atgattttgc caatacctgt attgctatac tattattaaa actatcacta
    28741 ctacttccta tctcaatatc caacataggt acagaatatt ctgtaattaa tttagattct
    28801 tcaccatatg ttgttccaga ccagtgagtt gcttctgtta atgtagtaaa ttcatcaagt
    28861 agattttcat tttttatatc ctcaatagca ttgattagat ttttaaagta tcttggattt
    28921 gaatttccaa attctcctgt tggaacatct ccaattgtat gagctgttag gatatgttct
    28981 ggagcatttt ctccctcatg ccaatttaca actcctgcaa aatcataatc tctaaaatgt
    29041 ctatttaaaa ttggaagata atatttataa ttatgactta ttacatcttt tgtttttaca
    29101 aaatgaaata agttattgtt tttatcctta aactctaata caaaattatc atcaataaca
    29161 atatcagttt gatttaaatc cattatttct tcacaacatt ttatcacatt actggctact
    29221 gggtctttct ttaaatcact gcaaaagaaa aaaactgctt ttttattatt catccataaa
    29281 tcacactcct ataacttaaa tataatatat cttcatacct aaaatcatct aaatatcact
    29341 ctaattccat taaattattt atatataata tactaccata ctcattttag ctataccttt
    29401 gcctgagttt ctatatatat gtgatacatc agctttaaat cttatagaat ttccactaga
    29461 tacaatgtaa tcttctccat ttattttaat ggttacttcg ccactaaaaa cagttataaa
    29521 ctcttgtgtt cctggaagtt cagcatttga atctaaattt ccaccttctt ctatttcaat
    29581 cataaatatc tcaaatcttc ttttggagtc aaatggaaat attggataac ttatatactt
    29641 tccattatct tcaataaaat ggtctatatc acttttttga atcaattcta tgtcactttc
    29701 ctgcctctcc atcaatgatg taaatgagat ttttagccca ttagttattt tcttcaaagt
    29761 agatatagta ggatttacct cacacttttc aatttgagat agcatactct tacttacacc
    29821 actaagttgt gcaatcttgt ctaaactaag atgtttttct ttccttatct tttttaaatt
    29881 ttcagcaatt atttcattta aactttgcat aaataccccc taatacatac taatataaga
    29941 taaattattt tgattattaa ataaacttag taatttaata gaaacttttt atccattata
    30001 atgtacatta tgtttaatat acattacttt ttttagaatg taaatagttt tctgaaattt
    30061 tgtgaaaatg aataaataaa aaactagtaa cctatatttt tacaaggtta ctagctttta
    30121 ttataataat ctttcatttt aactataaaa cttagatgca cctataagct gtctcttaga
    30181 aacaatctta gctaggtttg ctaatgaaga aatttttata tctttaaaag aaactaaaaa
    30241 tacaaactta aaatctgtaa attcttgatt tatattgtat atatcaaaat aacatctcaa
    30301 attatcaaat aagtaattat cctgtaagtc tgcttctatc tcatcagtag catcttttaa
    30361 atacgctctt acatcttcaa ataaaacact gaaataagac tcatctattt cttcacctaa
    30421 ctcttccaat atttcttcta tatctaaatc atccatatca aaagaatttc ctgtcaactg
    30481 gcaatcaaat accaatatat tttcttgatt tatagtattt gatatatctt gtacatattc
    30541 gaagtctgga cgtaattcta aaatgtcttt tatttcactg ctctttacat ggatttttcc
    30601 ttttctattt tcaattttta aatctatcat atgctccccc tttcaatcta caaatcttta
    30661 aataccttgt tttataatct tttcaatatc tatccaatta ctaactctaa ctatattttg
    30721 atgtttaaca tctttattat agttagtatc cagaagtatt actttaacac catcatcagc
    30781 tagttgcact gagtttaaag gattatcttc tataaatatg tcacaactca gttctcttgc
    30841 cttttcaact ttataatcac ttcctaataa gtaaacatct atatctgaaa atccttgtct
    30901 cgatagccaa tttttagtta catcagttaa acactcatgt ctagccgtta caaaagataa
    30961 attatgtcct ttagataatg tgctaattac ctctgtagca ccctctacaa tctttgcttc
    31021 atcatatgag tgtatatact tattattaaa ctccgcatac acatctctta ttcccgaacc
    31081 atacaattct tcccaattat gtgttataca ctctgcttca gttatatttt tattaaagat
    31141 atcatttagg tatggaagaa aatgataagg gcttgttatt gttccatcta tatcaataca
    31201 tatattcaat ttgttcattt ttacctccat gtaacattta ttgatttgat taatcaaata
    31261 ttatagcaca gctttaatta taaatcaaaa ctctggatat gactatacct atataatacc
    31321 aaaaagcttg catatttaag caagcttttc gtaaatttta catttttaac taatatataa
    31381 gtttttctaa ggttggttcc actccaaatt gtgatttcag ttctttatat tcttttaatg
    31441 ctttgtcatt attcgtttta gtttttatcg ctcttatcat taaatttttt ggtgtatcaa
    31501 gtggagatat gtattctact acagaagtat catatccatt gccctccaat aacaatgttc
    31561 ttaaaccatc agttattaaa tctgcaaatc tagctttaaa tacaccatgt tttaatattg
    31621 gttccatagc ttcatatcta tattgcccta atagctcctt atgacaacat ggtactacta
    31681 ctatagcttc agattttgct ctaattccta gacctattgc catatctgta gccgtatcac
    31741 aagcatgtag acttattaca aggtcaacat ccctattagg tgtataatta gtcaaatcct
    31801 cttttataaa tgacatattt ttataaccca agtttttagc catatttttt gatgcttcta
    31861 taactacatc tgaataatct attcctataa aataacagtt tttcttcaat acttctttta
    31921 tataaaaatt taagacaaag cttaagtagg attttccaca agcacaatcc aagatagtta
    31981 tacagtcttt atcctttatt tcttttaaaa tactatctat caattcaaca aaatggtcta
    32041 tctgattgta ttttcttatt ttatcatttt ttatcttacc atcttttgta agtactccaa
    32101 tttcttttaa aagtgcattt gcttgtccta cttttacata ataatctctg ttttttattt
    32161 gagatgccgt aaattcatct attttaggta cttcttcctt attatctaaa tattttacat
    32221 ttaccttctt attgtctcct tcaattacaa caatagttcc tcgctcaaca tatttaaata
    32281 ctaatccatc ataattttct gcctctttag aaatattctt aagcaactca ttaaacgtat
    32341 ataaatctga ttttccttta aaattaaatt tatatttatt atcttcctct attgcaattc
    32401 ctttaaactc ttttcttcca gatttaaaaa tcacctctaa ctcaataaaa aaatccctat
    32461 tatcaaggtt tctttgagta atccccataa aaaacatatt tagttttcct atactctgtt
    32521 tattcatcat aatctccctt ttaacttata tccttaaatc ttattaatta atattatttg
    32581 tttctgaatt atttatttct tcttttaaat cgttcaatct atttttaaaa ctttctatct
    32641 gcttatctga taaatcaaga tattctgtta tcttccctat atatttaggg cttttagtat
    32701 ttattaattt caaagcactt tccatagatt tttctctaag ctcatatgct ttctttacgt
    32761 catctctagc attactcaat gtttctgtat acttctcctt agaaagttct ggaatctgca
    32821 tattttcata ttcttttaca acttctttac acttgtttct ggcatttgta acatcttttt
    32881 ttatactaga taatttagtt atattttttg ctatatcctt agtgtaaggt atgcattctc
    32941 tcattggaac taaagctgct ctttctatat tataaatttc tttcttaaaa attcctatac
    33001 tttccatatc tatttttttt atatcttcac tatttttttc tttacttgta tatttattat
    33061 tttcttttgt attatcttct tgtttttctg atttattatt cactgaaaca ttactatcag
    33121 aattattctt ttcttttata tcattacttc cttcttcatt actttcttta ttttgtatat
    33181 ttctatcttg tttgttgttt aaatttttat tatttgatat ttcttcattc tttttattat
    33241 cctggtttaa atcagtactt tctttttcct taaaatttga tacatacttt tcatcatatg
    33301 tctcatcaga aatataattg tgataataca tagactcttt tattttatta agtccactca
    33361 tataaatatt acttatcatt attgttgtag ctaataccac ataaatacta ctgtagatac
    33421 aagacctctt aaaattcttg taatttctga atatcactac taaaggaata agatataaaa
    33481 atataaaaac taatatccac ctcataagtc gtgtctcctt tctcaaaacc gaaatccctc
    33541 aaatatttat ttttaatcat gtatcttata gtatttacca atttatgttt tttaatcgtg
    33601 aacttttatt aaattaaatt ctgtatagtc acaagatgct aatgtatatt ctactccatt
    33661 tcgaatcccc ttcaatccca ttttaaaaga ttctttacca tgcaagtgac cataaatcac
    33721 cttttcaaca ttatattctt caaataactt tgtaaataaa gattcttcca atttatcatt
    33781 cgtaggagga taatgagtaa tcacaattat ttttgagtga ccacttttac gagcagattc
    33841 taatgatagt ctaagtctat gttcttctct tttataaact ttttcatcag tctcatcaaa
    33901 tttaacatca ttaggacata tccagcccct acctccacaa attgcataat ctttatattc
    33961 ataaaaattt gtctgaataa aacgcatgtc ttcatatagc ttatttaaac ttgtaacggt
    34021 tgtccaccag taatcatgat ttccctttat aagtattttc tgacctggaa gtttgcttat
    34081 tatatctaaa tcttttttag cttcggataa attaattccc caagatgtat ctcctagtac
    34141 taagaccata tcttcttcct taacaacttc tttccaatta tctataattt tcttttcatg
    34201 accatcccaa ttacttccaa agatattcat tggcttattg actgatgttg aaaaatgtaa
    34261 atcacctata gcatacaaac tcataatatc attcctttct taattacact aaataggctg
    34321 tctcttttta gaaattattt tccattaaga ggcagcccca ttaatattta gcaaattttc
    34381 tacaagtagt ttttactgaa ctactttatc atctacctta gtatccttat attgctctaa
    34441 taatatatct atttcttgtt ttaattccaa aatttcagtt atatttttat ctgtttcagt
    34501 tctatcaagt tttctttggt actgcatagc tttatgtgta tcacccaagt catataactc
    34561 ttgtattcta tttagagtat ctctaatagc tatagacttc atttggtaca tagcctgagc
    34621 tttcattatg tcctctctta tttcactcat ttcttggtct aaaagaaagg cagcatcaga
    34681 tgctcttcta agcctttcag caagagtctc aggtatgtaa tggtcatatt tatattttaa
    34741 tgaatgttct attgtagccc agaaattcat tgcaagagtt cttatttgta tttcacatat
    34801 aatttccttt gaacccgcaa tggagtttat agggtatttt attataacat gatagcttct
    34861 ataaccacta tctttgtaat ttgtaatata atctttttct cctattatag tcatatcatt
    34921 tcttactttt atcaaatcaa ctatagcata tatatcctca acaaactggc acattattct
    34981 tataccagca atatcatcaa tttcagtttc tatatctttt gcatttatcc tttttaactt
    35041 agatattatg gaagatattt tttttgttct acctgtgaca aactctatgg gagaatattc
    35101 accttttgct aaatattctt ttcttatatt tttaaatttt atttttaatt cctctactgc
    35161 atgttcataa ggtgctagta tctcattcca tttttcgtat tccatattgc tcacccttta
    35221 tttgtttttt atgaccttct ttattttatc ataatttatt ttgtttttgt atagtaaagc
    35281 aattctccat tttccaatat ataccctatt tcttctaaat caactaagta acttatgaca
    35341 gatactaacg aagatttaaa aaaatgatac tctttataat tattgcttaa attattgtta
    35401 tttataatgt tttttaataa aatttctaaa gatttcggtt ctacaagcaa ctctcttatt
    35461 tgatataaat atttttttaa agcattcaaa tgtttttcta ttaatttata agaatcttct
    35521 ttcgatataa cttgcttgcc atgccctaaa actaagtact caaaatctat attttttaat
    35581 ttttctaatg aatttatttg ttcttgtata tcatatagaa acaagaaatc gtattttgat
    35641 aaaatttcat ctcctatcaa taaatcgcca acaaaaatca cattatcctt cgtcaaaaca
    35701 gctatactac caggtgtatg accctgaaat tcaattatat caaacatttc attgtttaat
    35761 tcaaattttc cagcttctat aaatttatct atttttatat catccaacat tttatttttt
    35821 aagatattat ccataaatac attgctcttt cccccaacaa tatactttga aaacaattct
    35881 ggattttcta tatataattt atcgtattca gatgacatta tacacacatc tttataatat
    35941 tctttaaatt gattacaagc tccatagtgg tcatcatgac catgagtatt tataatatat
    36001 ctaacctgta tattattttc atctaatact ttatttactc tcctaggtct tgaaccacat
    36061 aaccctgggt ctatcataag tgctgtgtta tcttcaaata catatattcc tgtattcgtg
    36121 ccacctttta taaaaaaggt atttcctttt acttttgtaa gattcacctt cttcacctca
    36181 ttttgacaat atttatgata caatttatta cttactaata taaaactata caagaacaat
    36241 cctttttatt ctatctatag ttctttttct tattttgtat tctttagata tccttatttt
    36301 cacattatat gctaaatttc tataaagtaa tgactctata aaatattcaa ctatacctct
    36361 atccctaaag tctatatcta tatttccact tgaataaata tttgagtcta atatcataag
    36421 ctcatcaaaa aagcttaatc tcttagattt acttaaataa tctttatttg gaataaatct
    36481 atcacaataa tctatcctgt aatttactac ttcatcattt atttcaaatc catattgctt
    36541 tttcattaac tctctatagt tattattttt ctcaataaca ttatctaaat ttttattata
    36601 atcctctttt ttccaatgtc caaacatcct acaatttaat ggtctaactt catatattaa
    36661 acatctatta ttagtatctt taaatggaca tgggttcttt cttgtatact cctcaaaata
    36721 ataatctaat attttactta tacagctttt ttttaaatca actctatctt caagataaca
    36781 aaatatattc aaaaactcaa ttaaatttat acctactgac tccatacaac aatttccaca
    36841 tccagaacaa tttcctactg gcaaggactt atatacatca tttagtttgt caaagagctt
    36901 atttttaatg gaataatcta tacaatttaa aatatcttta ttttttatac tagtcattat
    36961 ta
//
LOCUS       DAEPIN010000039        36571 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.039, whole genome shotgun sequence.
ACCESSION   DAEPIN010000039 DAEPIN010000000
VERSION     DAEPIN010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 36571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36571
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.039"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            <1..2859
                     /gene="tcdA"
                     /locus_tag="KR250_002112"
                     /gene_synonym="toxA"
     CDS             <1..2859
                     /gene="tcdA"
                     /locus_tag="KR250_002112"
                     /gene_synonym="toxA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_105161403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin TcdA"
                     /protein_id="HBH0068047.1"
                     /translation="ILQKIRIKGILSNTQSFNKMSIDFKDIKKLSLGYIMSNFKSFNS
                     ENELDRDHLGFKIIDNKTYYYDEDSKLVKGLININNSLFYFDPIEFNLVTGWQTINGK
                     KYYFDINTGAALTSYKIINGKHFYFNNDGVMQLGVFKGPDGFEYFAPANTQNNNIEGQ
                     AIVYQSKFLTLNGKKYYFDNDSKAVTGWRIINNEKYYFNPNNAIAAVGLQVIDNNKYY
                     FNPDTAIISKGWQTVNGSRYYFDTDTAIAFNGYKTIDGKHFYFDSDCVVKIGVFSTSN
                     GFEYFAPANTYNNNIEGQAIVYQSKFLTLNGKKYYFDNNSKAVTGWQTIDSKKYYFNT
                     NTAEAATGWQTIDGKKYYFNTNTAEAATGWQTIDGKKYYFNTNTAIASTGYTIINGKH
                     FYFNTDGIMQIGVFKGPNGFEYFAPANTDANNIEGQAILYQNEFLTLNGKKYYFGSDS
                     KAVTGWRIINNKKYYFNPNNAIAAIHLCTINNDKYYFSYDGILQNGYITIERNNFYFD
                     ANNESKMVTGVFKGPNGFEYFAPANTHNNNIEGQAIVYQNKFLTLNGKKYYFDNDSKA
                     VTGWQTIDGKKYYFNLNTAEAATGWQTIDGKKYYFNLNTAEAATGWQTIDGKKYYFNT
                     NTFIASTGYTSINGKHFYFNTDGIMQIGVFKGPNGFEYFAPANTHNNNIEGQAILYQN
                     KFLTLNGKKYYFGSDSKAVTGLRTIDGKKYYFNTNTAVAVTGWQTINGKKYYFNTNTS
                     IASTGYTIISGKHFYFNTDGIMQIGVFKGPDGFEYFAPANTDANNIEGQAIRYQNRFL
                     YLHDNIYYFGNNSKAATGWVTIDGNRYYFEPNTAMGANGYKTIDNKNFYFRNGLPQIG
                     VFKGSNGFEYFAPANTDANNIEGQAIRYQNRFLHLLGKIYYFGNNSKAVTGWQTINGK
                     VYYFMPDTAMAAAGGLFEIDGVIYFFGVDGVKAPGIYG"
     gene            complement(3194..3892)
                     /gene="tcdC"
                     /locus_tag="KR250_002113"
     CDS             complement(3194..3892)
                     /gene="tcdC"
                     /locus_tag="KR250_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin anti-sigma factor TcdC"
                     /protein_id="HBH0068048.1"
                     /translation="MFSKKNDGNEFSNEGKGSSKKIIKFFKSTKGIALLAFILGVFFG
                     NISSPACSEDHEEVISNQTSVIDSQKTEIETLNSKLSDAEPWFKMKDDEKKAIEAENQ
                     RKAEEAKKAEEAKKAEEQRKKEEEEKKGYDTGITYDQLARTPDDYKYKKVKFEGKVIQ
                     VIEDGDEVQIRLAVSGNYDKVVLCSYKKSITPSRVLEDDYITIRGISAGTITYESTMG
                     GNITIPGIAVEKIN"
     gene            4318..4563
                     /locus_tag="KR250_002114"
     CDS             4318..4563
                     /locus_tag="KR250_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Pathogenicity locus"
                     /protein_id="HBH0068049.1"
                     /translation="MGELQKIPGVGKATEKSLIMLGYTTIKSLKDANPAQMYEKECLI
                     RGQHIDRCQLYVYRCAVYFASTENPEPEKLKWWYWKD"
     gene            complement(4781..5050)
                     /locus_tag="KR250_002115"
     CDS             complement(4781..5050)
                     /locus_tag="KR250_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HBH0068050.1"
                     /translation="MFESRCGVCCDSCTKKEQVNCTGCPTMEKPFWGGECKVKTCCES
                     KELNHCGECDTFPCDMLLNKGKDQGFDPMVNIEQCRKWLEETVSN"
     gene            complement(5928..6665)
                     /locus_tag="KR250_002116"
     CDS             complement(5928..6665)
                     /locus_tag="KR250_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0068051.1"
                     /translation="MLRKSVLGEILKLKNSFIWYVLLALPLISVLIGSGNFYLNQGIL
                     KKEWYSLWTQVSLFYGEFFFPILIAIFCAYICRLEHMNHNWNNVMTLPVNLKNIFLSK
                     LTVVSILTAITQVFFVIFYIVAGNMFGFSGSVPKELTGWIAKGWIISISIASIQLYMS
                     IKIRSFATPIGISLCLSLFGMGLIVAKPAVGIFFPYSMLGSAMGVIKQSALNVNDTIV
                     TICVAFITTIVFSEIANRNFRKNDVIS"
     gene            complement(6677..7438)
                     /locus_tag="KR250_002117"
     CDS             complement(6677..7438)
                     /locus_tag="KR250_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0068052.1"
                     /translation="MKYLGLEFYKLKRKKIILMTFLFVAVEIMWCIINSNRAITRNPD
                     MLGGFEYSYMFMSFASLNGLFFPILISIITSRISDIEHKGDTWKLLKSSVTSLNSIYL
                     SKFLCSAILVSIPVLMQVLSIVLFGHFRGVIESISVSLLLKYTLGTILVSIAIIALHL
                     WIATVIPNQMIAITFGMIGSFIGLTSGLFYQGIRRLFIWSYYLELSPLSYAYDNVLGS
                     SVYRIDMNFSITILVFLIGMLIYYIGRNHLLKKEV"
     gene            complement(7438..8352)
                     /locus_tag="KR250_002118"
     CDS             complement(7438..8352)
                     /locus_tag="KR250_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0068053.1"
                     /translation="MEMIIRTQRLSKEYNKIFRVKDIDLRVPQGAVYGFLGPNGAGKS
                     TTLKMLLGLAKPTQGNINILGKELNEKNRISILKDIGSLIESPSYYGHLTGLENMTIM
                     QKLLDLPKKNVNEALKIVRLENQKNKKVSQYSLGMKQRLGIAMAIMKFPKLLILDEPT
                     NGLDPAGIEEIRELIKSLPEKYGMTVLISSHLLSEIDQIATSIGIINHGELVFQDSIK
                     ELHNRGQSQIAIKTKNVVSAQKLLNTKGYMSLIQDGYLTIENLKDSDVAKINGMLVHS
                     NIDVFRIEEHKKSLEDIFLELTGKAVTL"
     gene            8523..9230
                     /locus_tag="KR250_002119"
     CDS             8523..9230
                     /locus_tag="KR250_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0068054.1"
                     /translation="MESIKDKKILVVDDHKELLKMIDEILRKEGFSRVFLASSYEEAV
                     RVFRNVKPDCAILDVVLPDGDGFSIMRKIRETSKIPVIFLSARGEDEDRLIGLGLGAD
                     DYIVKPFLPKELTLRLVGILNRVYVPIEEEELPVFKLGDSVVVNLNSACVEKDGQEIS
                     LTAKEHSLLLKLYENKGRIVTNDSLCQAIWGDDMYGYENTLMVHVRRVREKIEKTPST
                     PKHLITVRGLGYKLMIN"
     gene            9275..10681
                     /locus_tag="KR250_002120"
     CDS             9275..10681
                     /locus_tag="KR250_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBH0068055.1"
                     /translation="MDSVKYILRRFIGSSILISLLFIVLNIAGFLLFSYMLFVSKYSD
                     NLDTSDSREIKSNLIMIEDNLILRNGKFILDDSSKEVLHKNKVWAMLIDNNGDRIWGF
                     DLPKEIPSHYTLTDVAKFSRFFLKDYPVYVWEHPNGILVTGYPKDKYTKFNLTYTVKE
                     IEQFPMLIIGMFLVNAIVTFLIALFIGLKMVKSIKPIITGIKLMSKGEPVLLQEKGIL
                     SDISKSINTVSKELQMKDEKLRKKEEARSNWIAGISHDIRTPLSMIIGYAGELEESSS
                     LSNREQEQVSIICNQGIKIRELVNDLNLVSKLEYNMQALNCDKIRVSAFLRELISEFI
                     NNNLDNDFYVEFDILNEDIIIDADKKLLKRAISNLIQNSITHNQQGCNIRVTSKSDLD
                     FCYIIVEDDGKGMSQERINSLLKSQNGLSEAYAKGQNHGLGILIVSGIINAHRGKLDI
                     TSIDSQGLKTILKLPLIK"
     gene            12480..12869
                     /locus_tag="KR250_002121"
     CDS             12480..12869
                     /locus_tag="KR250_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBH0068056.1"
                     /translation="MTIKKLPQSELKIMKFIWKSDSKVTSRDIVLGMEQKYQWKQTTT
                     LTLLSRLVVKRFLNSQKIDKYTHYEVLIKEKEYIGVETRDFFRNIHDSSIKSLLLSLH
                     ENINLSKDDILLIEEWIKNLKEEEKDV"
     gene            13603..14073
                     /locus_tag="KR250_002122"
     CDS             13603..14073
                     /locus_tag="KR250_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/dioxygenase
                     family protein"
                     /protein_id="HBH0068057.1"
                     /translation="MKYQVSLLAVKDIEVSKKFYKELFEQEIELDLGKNVTFKGGFAI
                     QEDFAWLTGINPDTIIQKSNNMELYFEVDDFDEFIEKLNYYKDVEFVHKPLKHEWQQR
                     VVRIYDPDKHIIEIGESMEVIARRYLSEGYSVEETSKIIQHPIKFVKQCENSKE"
     gene            14377..15246
                     /locus_tag="KR250_002123"
     CDS             14377..15246
                     /locus_tag="KR250_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HBH0068058.1"
                     /translation="MVIIKAIELYKKLDLEFNIKNINDDWSFMNFENDFITPEFRKKY
                     IGLVLDNAQNINKVYTTTFPDKEIIKQVIDKDEKDILIFSHHAMGYIASDEGFPFHDI
                     PLSYMEEMKNRRISFYVLHSPLDNYSDYSTSVSFAKAMGLEIVKPFCKYDDKIEVGVI
                     CKTSLKSIEEIKELIKKSVGHDVKLYDYGDSVLKDGLVAIAAGGGSYPFVAREVAELG
                     INLYITGFTKPLKHFEPVLEFHQIAKDNLINVIGATHYSTEKFACMGMVDYFKNLGLE
                     SEFLQGKYYLEDL"
     gene            16121..16828
                     /locus_tag="KR250_002124"
     CDS             16121..16828
                     /locus_tag="KR250_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBH0068059.1"
                     /translation="MIFTKSNKYDKDFLMKNMMGPNCIKILEELTSKIKLEKGMRILD
                     LGCGKGISSIFLAKEFDATVFATDLWIEPTENYKRFKEFKLDDKIFPIQAEAHELPYA
                     EGFFDAVISIDSYHYFGNKEGFLDNHISPLVKEGGILAMAMPGLKEDFVDCIPDELIP
                     FWQDNMNFHSITWWNKLWSESESVIVEKCEALNCHDEAWKDWINCDNSYAINDKKMME
                     VENGKYFNTISLIARTK"
     gene            17427..17927
                     /locus_tag="KR250_002125"
     CDS             17427..17927
                     /locus_tag="KR250_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBH0068060.1"
                     /translation="MIEGIKFIKAEEKYIYSYWQTFDKIAKERKYLAMDEAFPFEETV
                     EFIKNIINKNLPQLFIIDLESDNCIGWCDVLPKTEKVGYLGMGILKEYREKGIGSNLL
                     KQIIDLSKEYGYEKIELDVFKSNSRAIHVYKSLGFVEVNTISSGFTWNDRPVKEEVIQ
                     MELTLI"
     gene            complement(18177..18956)
                     /locus_tag="KR250_002126"
     CDS             complement(18177..18956)
                     /locus_tag="KR250_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0068061.1"
                     /translation="MNKNILNNSFPEVPESFHNSLSKTLNSLPEREENYKMINNKTHK
                     LPFRKGLVATLAITLILSTTALAVGHIYSLVGKSSTKATYTSIPTTEQVKKDFNFTPN
                     IVSEFSNGYKFKGAYTVNKKAFDKENNVLGKSKALSFEYKNGNDEISLDTANNVLGKS
                     SDNLKVVDTYKDINISYEAFAQKFVPEGYKMTEQDKIDEKSGKYTFSVGPNNESDKVE
                     VNKFKFLTWKQDGVYYSFTVQDSNLSMNDLVKMAHEVIDAK"
     gene            complement(18953..19432)
                     /locus_tag="KR250_002127"
     CDS             complement(18953..19432)
                     /locus_tag="KR250_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBH0068062.1"
                     /translation="MDKTTFTNNILESEQTLYRVSKSILGNDQDCEDAVNNAILKAYE
                     KLDSLKEEQYFKTWLIRIVINECNSLRRKRLKSLSFEDVFKNKKIDEKDDYSDLYTAI
                     QSLPKKIKIPIVLYYIEGYSVDEVKEILDIPQGTVKSRLSRGRRLLKTKLENTEVII"
     gene            19931..20221
                     /locus_tag="KR250_002128"
                     /pseudo
     CDS             19931..20221
                     /locus_tag="KR250_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426892.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            20418..21425
                     /locus_tag="KR250_002129"
     CDS             20418..21425
                     /locus_tag="KR250_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HBH0068063.1"
                     /translation="MGKYQDSKKVVRNYFEALENATGDEVENVLKQHMKSDYNWKGVY
                     PFREQEGTENVADVFWKPLKKSLSNMQRRQDIFIAGTNEIDNSEWVMSMGHFMGLFDN
                     DWLGIKHTRKMISLRYAEFNCVQDGKIAKTGLFVDIIGFMIQAGVNPLPPQTGSYFVY
                     PGPIDHNGLLFEDADEVEGVKTLALVNKMVDDLSELNESGAMGCPPEILEKSWAKDMI
                     WYGPGGIGASYTIPRYQEQHQLPFRNNLKGKTFNGHVCRFAEGNFACFFGWPNLTNVP
                     CGGFLGLPGGEVKADMQVVDVYYRKGDKLQENWVLIDIPYWLKQQGLDILERTKSIFN
                     A"
     gene            21463..22890
                     /locus_tag="KR250_002130"
     CDS             21463..22890
                     /locus_tag="KR250_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HBH0068064.1"
                     /translation="MASTNKNNATSKEEEKEVSTSGLESCTVKENKAKMNQTNFSNIN
                     ENQTNENKTNTYKRNIDKGNIDKGNIDKTNTNKSDINKVNIDTVDTDKLNTNTLTKKN
                     KKVIFKGEDNMSSTKIYYGDSLEVTPVGVMDYNDFSKQTKREQEIPGFDSKYRDFVDY
                     IMKITHNIWEEKGIGVIYDTYHNNVTMHCGSSNLVGIKDVISNTLQTLHAFPDRRLIG
                     QNVIWSNFGADGFLSSHRVLSTATNLGDSNFGPATGRKINFRTVIDCATTNNRIHEEW
                     LVRDNLWIVTQLGLNPQEVAKSMAKASESKVLSLQSTYGICESMDGQFMPTKYQAKDD
                     SAGEMMLEMTSRIYNYKYINEVKKYYHDNAVVHFICDKDLNGYDEIQGMIVSLLASIP
                     NGSYEVERVTCNQREKNEGYDVSVRWRLRGINEGIGFFGQPSGKHIEVMGINHYHILQ
                     GKVKEEWITFDGMDVLKQMYMGVEE"
     gene            23032..24297
                     /locus_tag="KR250_002131"
     CDS             23032..24297
                     /locus_tag="KR250_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBH0068065.1"
                     /translation="MKTTVEIENDIILGKDYSNLKRGIAFFSIALIYFFYCYNFMVGT
                     FIKPTMIYALADGGFGFSLKQTEEIFAVMSFGTIPGTFIFGVISTKIGKKRTLISVAL
                     LIGLTTFIPMLSPTNIMLWKIARLCTGVVLGGVFGTAMPLVADMFPSKYRGKLAAILT
                     ALFSLAMIFGGKVYGVLGDANWQILMYTAIIPPIVGAILAIFFVPDDLEYTKELVKKG
                     KETGEKISYISMYKGKYLWIGLGTILLSGANFTAYSAFSNNATTYLVTRIGMSAAVAG
                     SIYSLQGIGQLVGYLFWGSIADKFGRKIPFIGMALAAVFVFIYTKLGGDNTTTFYMIS
                     VLLGVAVGYSGAWGAYYTELFPSKYRSLSSGMSFNGGRIISTFAIPAIAGTASGTLGM
                     MTIFYISIAVFVAGAIVWLFLPETLNNKK"
     gene            24339..25937
                     /locus_tag="KR250_002132"
     CDS             24339..25937
                     /locus_tag="KR250_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HBH0068066.1"
                     /translation="MISSIDVCIIIGYLLLMLVIGYYSGKDNKNQEDYFLAGRSMPWI
                     PIALSVAATMISANGFIGGPGWAYVDGMYPVMVNITVPLAIFFALSITTPVIYKLKIT
                     SIYEYMGLRLGNYSRGLTIIQFFINSLIQASSMVYIPVLIIQMITGWSLYALVPIVVL
                     VSIIYTLLGGIKAVIWTDSIQMIVVISAVFIVIIVALKGMNLSFFDTLQIAQQSGKLN
                     TFDFSRDISVTNTFWATLIGGTVMWIRYFCFDQSQVQRVLTSKSLKCAKNSFVVSAFV
                     MNLVYYFMLLVGVILFVFYGGRTFETSNEIMITFILNELPVGAIGIIIAGVFAAAMSS
                     IDSILNSMTTVFTKDIYEFYFKKDNKESSLKVTMIITVVIGVVMTGFIIMGFGGSIKS
                     ILDLVGNYISYFAGPATGAFVLAMFTYKANDKGVSIGFIVGLVLGFFISNKYNVSWLW
                     NPAIGATITLIFGYIFSVILKKNHNADDIKKYTAFGMRNHLISQGMDKVDGVSIVPFS
                     LGKQELIVLAFFIIQYIILFLIQF"
     gene            25972..26994
                     /locus_tag="KR250_002133"
     CDS             25972..26994
                     /locus_tag="KR250_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBH0068067.1"
                     /translation="MNQKKVHYNKSATSKDVARLAGVSQSSVSRAFGSANGKGVKPEV
                     RERIFRVANEIGYVPNLVARGMISGKTNVIGLIVGDSLGPFYNRIINLFVEKIQEIGK
                     QCLVFKVPRQQSIDSIISKVIQFQVEAVIITASAMNKVMAETCEENNIPVILFNRFIP
                     GIKISTVYVDPIEGGGMAAEYLLKKGHKNIGYIQFTRETSEEMEKKIGFYSKLRQSGI
                     HNLKEESANYDYDSGYEAGKRMLALSNPPTAIFCTSDLIAIGVMDAARFEYKLRVPED
                     LSVIGYDDIQMASWKSYNLTTIRQPLDLLIEKTINILKSLLNEENPESVVEMLKPELI
                     ERGSTL"
     gene            complement(27382..28785)
                     /locus_tag="KR250_002134"
     CDS             complement(27382..28785)
                     /locus_tag="KR250_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBH0068068.1"
                     /translation="MKMLNNFFKKINNPAPVFAQTKMERDNQTDDSLSTKPKTTFRDV
                     AGLDEVKEELFEIVDFMKSPQKYQKMGAKIPKGVLFYGPPGTGKTLLASAVAGETNSS
                     FFNVTGSEFVEKYVGVGAKRVRTLFEKARKEAPSIIFIDEIDAVGAKRHLESNNEKDQ
                     TLNQLLVEMDGFNKDSNVLIIGATNRLDLLDEALLRPGRFDRHIHIGAPNYHTRFEIL
                     KVHTDDKPIDKSVNLELLAKKTHGFNGAHLSNIANEAAIFAVRDDSECITSEHFDKAL
                     ERVIAGLESKNSALVEKEKKIVAYHEAGHALVSDIVGICPIQKISIVPRGQALGYVLQ
                     LPDEDRYIYTKDELIGKIKILLAGKASEELIFNHKSTGAKDDLKKVTEIANQMVCEYG
                     MSNLGFMTIDGNDKTFLCDKVQKEANRIVEICYKETLEMLKDNLEDLHSVSKFLFEKE
                     TMTHEELKDLIGKEAVN"
     gene            29652..30161
                     /locus_tag="KR250_002135"
     CDS             29652..30161
                     /locus_tag="KR250_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009528606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HBH0068069.1"
                     /translation="MSKELAINEQIKDKEIRVLSPTGEQLGVMPTKEAQAMANSKNLD
                     LVQISPNANPPVCKIMDYGKFRYEQARKEKEAKKKQKTIVVKEVRLRPGIEQNDLNTK
                     ANNAIKFLKKGDKVKVELRFRGRELGHKDIGKEVMLKFLDIIKEFGEPTKAPAFEGNN
                     MVVIIDPKK"
     gene            30189..30383
                     /gene="rpmI"
                     /locus_tag="KR250_002136"
     CDS             30189..30383
                     /gene="rpmI"
                     /locus_tag="KR250_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HBH0068070.1"
                     /translation="MPKMKTHRGAAKRLKKTGTGKLKRAKAFKKHILTKKSAKTKMNL
                     RKSTLVSDGDAKRIAQLLPY"
     gene            30439..30795
                     /gene="rplT"
                     /locus_tag="KR250_002137"
     CDS             30439..30795
                     /gene="rplT"
                     /locus_tag="KR250_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HBH0068071.1"
                     /translation="MARVKKAMNARKKHKKILKLAKGFRGSRSKLYRPANTFVMKALK
                     NAYIGRKLKKRDFRKLWIQRINAAARMNGISYSRLMNGLKLSGIEVNRKMLSEMAIQD
                     PEGFAKLAEVAKAKLA"
     gene            31105..31650
                     /locus_tag="KR250_002138"
     CDS             31105..31650
                     /locus_tag="KR250_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0068072.1"
                     /translation="MNEKLMDCFVNPIKCKLLIEIFSNNRTTAKQLGEKFQDISKATL
                     YRYLNKMLKDDIIKVVEENKIRGTVEKVYAMKLDIADDVNKIIDENSGEEYAQLFTQY
                     MMGFLREFYEYAERKEKDILKDGSGFSVCPVYASIDEIVEVSKKIASILEPLRNNSHE
                     EGRRMHSIGVIITPPKEDEGK"
     gene            31637..31801
                     /locus_tag="KR250_002139"
     CDS             31637..31801
                     /locus_tag="KR250_002139"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0068073.1"
                     /translation="MKGNKSDLHEVEPIYTENRFMIYICIQSKVSLLGLYFIVSFHLS
                     NDLEWYNEVF"
     gene            31814..31990
                     /locus_tag="KR250_002140"
     CDS             31814..31990
                     /locus_tag="KR250_002140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0068074.1"
                     /translation="MPKEMNFRPHMQLGYIIQLDQIYKIKISIFIADKLTVILLKNHL
                     LVIVSILKEIIGIY"
     gene            32090..34015
                     /locus_tag="KR250_002141"
     CDS             32090..34015
                     /locus_tag="KR250_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBH0068075.1"
                     /translation="MKIKFVIMPLVIFLMVLFVGCQSTKDTEVKAKYTEIKILGTGDL
                     HSILTDSMVSYVNEEREKNKNLLMVDAGDFYGTQSREMWEWSSGKKLINIKRNGRAEY
                     DDIIKSSKDEVPIVKDMAKLKYDAVVLGDNEFVSNDKQSLDKLVSDFKNNNMPLVSAN
                     IYEQSGENYVQPYVMKNIKTDEGNVKVGILGLTIKEVGETLGLEDFEKDKKARELGEQ
                     ADYKGKLYANDLVEDAKKWIKVMEKESPDIIVAVVHSGEEPKTDRNPGNRIKELATTV
                     DGIDAIVAGHTHEKIDEHKYKNKSGDEVIVTQPGWHGDRVSEINFKLNKENGKWDIKE
                     KSSKLKIIDKEVSIVATADLHSHFSDKLTVDLVNERSNPIEPVVVDAGDFLDPQTDEM
                     TQWYKEWKSIRDNNSDKTISRCPMVMDMNQGMYDAVVLGNHEFVSEKDEFWSDERLLD
                     AVIEDFEQVAISVLSANIYRQSGKNYVKPYIIKDVETSEGNVKVGILGLTIKEVGKGL
                     KNVNLQEQFQYKDKLYANDLVEDAKKWVKVMKKKKPDIIVAVVHSGEEPKNPKHPGNR
                     IKELATTVDGIDAIVASHTHEKIDEHEYKNKSGEKVIVTQPGEHGKYYSKITFKVNKE
                     KGSWIINDKFSKTIEVE"
     gene            34416..36320
                     /locus_tag="KR250_002142"
     CDS             34416..36320
                     /locus_tag="KR250_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBH0068076.1"
                     /translation="MKIKFIIVASIIFLIVLLVGCQSTKDTEINVLATSDLHSIIPDN
                     LISYVEEERKYDENLLMVDAGDFFDMQSGEMNKWFTGQKLVRFKDGIPEYKKIAEPRE
                     GEVPLAKKMAKLKYDAVTFGNHEFVSNDKQSLDKLVSDFKNNNIPLVSANVYEQSGEN
                     YVKPYVMKNINTDKGTVKVGILGLTIKEVGERSRFKSFEQDKKARELGEQPQYKGKLY
                     ANDLVEDANKWVKVMEKKEKPDIIVAIVHSGEEPKNPKNPGNRIKELATTVEGIDAIV
                     AGHTHEKIEQHTYKNNSGEEVIVTQPGAHGNSISKINFKLDKKNDKWFIKDKYSELKI
                     FGKEVNIITTSSLNPKFSDEMAVNLFNERDRDVQPIFIDTGDFLNTNTKEMTKWAKEW
                     QYNENKGIYKKINEWPIVYLGYDAVVLGSHEFTEDKETFEEKKSTLDYIIENFEEMKI
                     PVLSANIYDESGENYVKPYIIHNVETREGNIKVGVLGLTTTDKDEEDLSSSSLSKDSK
                     TSKIPEETGKLYTNNLVKDASEWVKVMQKESPDVIVAVVHSDNNSTLKNPSSEVKKLA
                     MSVDGIDAIVVGHTKNKVEEHIYKNKSGDEVIVTQSGEDCYSKISLELRKENGKWNII
                     NKYSKAIKMK"
BASE COUNT        14086 a         4076 c         6020 g        12389 t
ORIGIN      
        1 atattacaaa aaataagaat caaaggtatc ttatctaata ctcaatcatt taataaaatg
       61 agtatagatt ttaaagatat taaaaaacta tcattaggat atataatgag taattttaaa
      121 tcatttaatt ctgaaaatga attagataga gatcatttag gatttaaaat aatagataat
      181 aaaacttatt actatgatga agatagtaaa ttagttaaag gattaatcaa tataaataat
      241 tcattattct attttgatcc tatagaattt aacttagtaa ctggatggca aactatcaat
      301 ggtaaaaaat attattttga tataaatact ggagcagctt taactagtta taaaattatt
      361 aatggtaaac acttttattt taataatgat ggtgtgatgc agttgggagt atttaaagga
      421 cctgatggat ttgaatattt tgcacctgcc aatactcaaa ataataacat agaaggtcag
      481 gctatagttt atcaaagtaa attcttaact ttgaatggca aaaaatatta ttttgataat
      541 gactcaaaag cagtcactgg atggagaatt attaacaatg agaaatatta ctttaatcct
      601 aataatgcta ttgctgcagt cggattgcaa gtaattgaca ataataagta ttatttcaat
      661 cctgacactg ctatcatctc aaaaggttgg cagactgtta atggtagtag atactacttt
      721 gatactgata ccgctattgc ctttaatggt tataaaacta ttgatggtaa acacttttat
      781 tttgatagtg attgtgtagt gaaaataggt gtgtttagta cctctaatgg atttgaatat
      841 tttgcacctg ctaatactta taataataac atagaaggtc aggctatagt ttatcaaagt
      901 aaattcttaa ctttgaatgg taaaaaatat tactttgata ataactcaaa agcagttacc
      961 ggatggcaaa ctattgatag taaaaaatat tactttaata ctaacactgc tgaagcagct
     1021 actggatggc aaactattga tggtaaaaaa tattacttta atactaacac tgctgaagca
     1081 gctactggat ggcaaactat tgatggtaaa aaatattact ttaatactaa cactgctata
     1141 gcttcaactg gttatacaat tattaatggt aaacattttt attttaatac tgatggtatt
     1201 atgcagatag gagtgtttaa aggacctaat ggatttgaat attttgcacc tgctaatacg
     1261 gatgctaaca acatagaagg tcaagctata ctttaccaaa atgaattctt aactttgaat
     1321 ggtaaaaaat attactttgg tagtgactca aaagcagtta ctggatggag aattattaac
     1381 aataagaaat attactttaa tcctaataat gctattgctg caattcatct atgcactata
     1441 aataatgaca agtattactt tagttatgat ggaattcttc aaaatggata tattactatt
     1501 gaaagaaata atttctattt tgatgctaat aatgaatcta aaatggtaac aggagtattt
     1561 aaaggaccta atggatttga gtattttgca cctgctaata ctcacaataa taacatagaa
     1621 ggtcaggcta tagtttacca gaacaaattc ttaactttga atggcaaaaa atattatttt
     1681 gataatgact caaaagcagt tactggatgg caaaccattg atggtaaaaa atattacttt
     1741 aatcttaaca ctgctgaagc agctactgga tggcaaacta ttgatggtaa aaaatattac
     1801 tttaatctta acactgctga agcagctact ggatggcaaa ctattgatgg taaaaaatat
     1861 tactttaata ctaacacttt catagcctca actggttata caagtattaa tggtaaacat
     1921 ttttatttta atactgatgg tattatgcag ataggagtgt ttaaaggacc taatggattt
     1981 gaatactttg cacctgctaa tactcataat aataacatag aaggtcaagc tatactttac
     2041 caaaataaat tcttaacttt gaatggtaaa aaatattact ttggtagtga ctcaaaagca
     2101 gttaccggat tgcgaactat tgatggtaaa aaatattact ttaatactaa cactgctgtt
     2161 gcagttactg gatggcaaac tattaatggt aaaaaatact actttaatac taacacttct
     2221 atagcttcaa ctggttatac aattattagt ggtaaacatt tttattttaa tactgatggt
     2281 attatgcaga taggagtgtt taaaggacct gatggatttg aatactttgc acctgctaat
     2341 acagatgcta acaatataga aggtcaagct atacgttatc aaaatagatt cctatattta
     2401 catgacaata tatattattt tggtaataat tcaaaagcgg ctactggttg ggtaactatt
     2461 gatggtaata gatattactt cgagcctaat acagctatgg gtgcgaatgg ttataaaact
     2521 attgataata aaaattttta ctttagaaat ggtttacctc agataggggt gtttaaaggg
     2581 tctaatggat ttgaatactt tgcacctgct aatacggatg ctaacaatat agaaggtcaa
     2641 gctatacgtt atcaaaatag attcctacat ttacttggaa aaatatatta ctttggtaat
     2701 aattcaaaag cagttactgg atggcaaact attaatggta aagtatatta ctttatgcct
     2761 gatactgcta tggctgcagc tggtggactt tttgagattg atggtgttat atatttcttt
     2821 ggtgttgatg gagtaaaagc ccctgggata tatggctaaa atatatgttt gataaaaaat
     2881 tattcctgtg ctactaagaa attattttta tataataaat attgagattt aattaaaagt
     2941 catgtgttat tgtaatacat gacttttagt taaaattttt ctatcattta ataatctatt
     3001 attcttgact attttataat aaaattcata tatggaaata ttaatactaa ataattaata
     3061 gttgataaaa aatagataat atgctaaaag caaaaactaa tttagagcct tgtaactgtt
     3121 tatttgcaat tataaaaaca tctttaaaca tattgactat aatataaaat attaactata
     3181 atacaaaaca atattaatta attttctcta cagctatccc tggtatagtt atatttccac
     3241 ccatagttga ttcataagtt atagttccag cacttatacc tcttatagtt atgtaatcat
     3301 cctctaatac tcttgaagga gttattgatt ttttataact acatagtaca accttatcat
     3361 aatttccaga cacagctaat cttatttgca cctcatcacc atcttcaata acttgaataa
     3421 ccttaccttc aaattttacc tttttgtact tataatcatc aggtgttcta gctaattggt
     3481 cataagtaat accagtatca tatcctttct tctcctcttc ttcttttttg cgttgttctt
     3541 cagccttttt agcttcttca gcttttttag cttcttcagc tttacgttga ttttcagctt
     3601 caatagcttt cttttcgtcg tctttcattt tgaaccatgg ttcagcatca gacaatttgc
     3661 tatttaaagt ttctatttct gttttttgag aatctataac tgatgtttgg ttagaaatga
     3721 cctcctcatg gtcttcagaa caagctggtg aggatatatt gccaaaaaac acgcctaaaa
     3781 taaatgccag tagagcaata ccctttgtgc tcttaaagaa tttaattatt ttcttagagc
     3841 ttccttttcc ttcattacta aattcgttac catcattttt tttagaaaac ataatacaat
     3901 ctccctttta ttagatatat tttattttaa aagttaaaaa cttaaaataa ctagttgact
     3961 aaaaattata tattcatatt ttcctaaaat atatttcccc taagcataat tttacaaaat
     4021 aaagtaagaa gattcaatat attttgaaaa aataagataa aaatgtcaga ttataatata
     4081 attttcttga aggtgaattt tatatattaa attaaacata aagtatttta tttgtataaa
     4141 actatattat gatgtaatta agtgactcag tgatttagag gtggtaaaat atatatatat
     4201 gaaagagcat aaataaattg atataataaa aaattaaata ttaaatatta actataataa
     4261 aaaaatatct ggtaataggt ataatataaa aagaatatac cagaaagaag gtgaagtatg
     4321 ggagaactac aaaaaattcc aggggtaggg aaagcaacag aaaaatcttt aatcatgtta
     4381 ggatatacaa ctattaaatc acttaaagat gcaaacccag cacaaatgta tgaaaaggaa
     4441 tgtttaatac gtggacagca catagacaga tgtcagttat atgtatacag atgtgcagta
     4501 tattttgcat caactgaaaa tccagaacca gaaaaattaa agtggtggta ttggaaagat
     4561 taaaaaatca aaaaagttag acataaaaaa gagttgaata tccgttttaa ataataaaaa
     4621 ctaatactca actctttttt agtcgtaaat tttatgtaaa tctatttgta tggctaaata
     4681 tacccatcaa taaatgtata aatataccaa tcaacaaatg actaaacata ctacctaaca
     4741 aaggattaaa taaattactt aacaaagcat tctaaaacaa ttaattagaa actgtttctt
     4801 caagccattt tctacattgc tcaatattta ccatagggtc aaaaccttgg tcttttccct
     4861 tatttaaaag catatcacaa gggaaagtat cacattcacc acaatgatta agttccttac
     4921 tttcacagca agttttaacc ttacattctc caccccaaaa tggtttttcc atagttgggc
     4981 aaccagtgca atttacttgt tctttttttg tacaactgtc acaacagaca ccacatcttg
     5041 attcaaacat gttcaaacat cctttccatt atttaaaaat attataaaat gattttagat
     5101 gaatacaaaa gtgcaaatag ttgattttag gtcatctaaa tgttaaacat aaaattatta
     5161 gtactggtaa atttataaaa actattccaa aaatcttttt gaatatttca aaacacaata
     5221 aattacttca aatccctttg aatatatttt acagcattat agattgcttt aaatctcttt
     5281 gaatattcta caagactata gattagttta aatccctttg aatatatttt acaacattat
     5341 agattgtttt aaatctcttt gaatattcta caagactata gattagttta aatccctttg
     5401 aatatttcaa aacaccatag attactttaa atccctttga atattctata acactataaa
     5461 ttacttttaa cttttatata accataaagt ttttatctgg cactttaaat ggagttttat
     5521 atcaaatttg ccttccatcg aataaccaaa cttaatttaa ttacacattc caagagtcaa
     5581 atattcattc aatatatttg ataattctat gttatagcta taattaaata tagattatta
     5641 aagttaaata tttatttaat atattgaatg attttttacc tatatttaat taagtttcat
     5701 tatataatat aaaaattgtt tttgcattaa gttttaatat aatacataat aattttaaat
     5761 aataacatta ttatatattt tttaatttta ggagtatatc tattatatag cgagtagata
     5821 aaacatataa aattcaaata aaagatatat atttttaaat aaaatggata tagatttcaa
     5881 ctaaaaagaa tataaatttc aaaccccaaa atatctagca tttaccatta agaaataaca
     5941 tcattctttc taaaatttct attggcaatt tcagaaaata ctatagtagt aataaatgca
     6001 acacatattg taactatagt gtcattaaca tttaaagcag attgctttat aacccccata
     6061 gcactaccta acatagaata agggaaaaat ataccaacag caggttttgc tactattaat
     6121 cccataccaa atagagaaag gcaaagacta ataccaatag gagttgcaaa acttctaatc
     6181 tttatagaca tgtataattg tatagaagct attgaaatag aaataatcca acctttagca
     6241 atccaaccag tcaattcttt aggtacacta ccagaaaaac caaacatatt tcctgcaact
     6301 atataaaaga taacaaagaa aacttgtgta atagctgtaa gtattgaaac tactgtaagt
     6361 ttagataaaa agatattttt taaattaact ggcagtgtca ttacattatt ccaattatga
     6421 ttcatatgtt caagcctaca aatatatgca caaaagattg cgattaagat aggaaagaaa
     6481 aattccccat aaaaaagact aacttgagtc cataagctgt accattcttt tttgagtatg
     6541 ccttgattta gataaaaatt accagaacca atcaacacac ttataagtgg caaagctaat
     6601 aatacatacc atataaaaga attttttaac tttagaattt ctcccaaaac tgacttcctt
     6661 aacatttaaa tccctcctat acttcttttt ttaataaatg atttcttcca atataataaa
     6721 ttagcattcc aattaagaaa actaaaatcg taatagaaaa attcatatca attctataga
     6781 cagatgaacc taaaacatta tcataagcat aacttaaagg tgataactct aggtaataag
     6841 accatataaa caatctacga ataccttgat aaaacaaacc tgatgtcaaa cctataaaac
     6901 taccaatcat gccaaatgta attgcaatca tttgatttgg aattacagtt gcaatccata
     6961 ggtgtagtgc aatgatagca atacttacaa gaattgttcc taatgtgtat tttaaaagta
     7021 aagatacaga aattgactca ataacccctc taaaatgtcc aaacaacaca atagataaca
     7081 cttgcatcaa tacaggtata gataccaaaa tagcagaaca taaaaattta gataaataaa
     7141 tactatttaa agatgtcaca gaagatttta aaagcttcca tgtatctcct ttatgttcta
     7201 tatcgcttat acgtgatgta attattgata ttaagatagg aaagaataga ccatttaaac
     7261 ttgcaaaact cataaacata taggaatatt caaatccacc taacatatct ggattacgtg
     7321 taatagctct atttgaattt atgatacacc acattatttc aacagctaca aataaaaagg
     7381 tcatcagaat aatcttcttt cttttaagtt tataaaattc tagaccaagg tatttcatta
     7441 taaagtcacc gccttaccag taagttctaa gaatatatct tccaagcttt ttttatgttc
     7501 ttctattcta aatacatcta tatttgaatg gacaagcata ccatttattt tagccacatc
     7561 tgagtctttt aaattttcaa tagtcaagta cccatcttgt ataagtgaca tatatccttt
     7621 agtatttaat agtttttgtg cagatacaac attcttagtt ttgatagcaa tctgactttg
     7681 acctctatta tgaagttctt ttatgctatc ttgaaaaacc agttcgccat gatttattat
     7741 tcctattgat gtggcaattt ggtcaatttc acttaaaaga tggctagaga ttagaacagt
     7801 catcccgtat ttttctggta gagattttat taattctcta atttcctcaa tccctgctgg
     7861 gtcgagtcca tttgtaggtt catcaagtat taataatttg ggaaacttca ttattgccat
     7921 agcaatacca agacgttgct tcatacctaa agaatattga ctaacttttt tatttttttg
     7981 attttctaac cttacaattt ttaatgcttc atttacattt tttttaggta aatctaagag
     8041 tttttgcatt atggtcatgt tttcaagccc tgtaagatga ccatagtatg atggagattc
     8101 tatcaaagaa cctatatctt ttaaaattga tattctattt ttttcattta actctttacc
     8161 taaaatatta atatttcctt gtgtgggttt agcaagtcca agaagcattt taagagtggt
     8221 agattttcca gctccattag gacccaaaaa accatataca gctccttgtg gaactcttag
     8281 gtctatatcc tttactctaa atattttatt gtattccttt gaaagtcttt gtgttctaat
     8341 tatcatttcc atatgtttca tccccttttt taatctgtgc ttattgattc ttttaactta
     8401 tatcagtata ataacttggt tacatttcaa ttagctgaaa tttaccttac aataacctta
     8461 aaagtctatt taggcaaagt tgtgaaaatg ggtatatggt atactaaagg agggtgatga
     8521 ttatggagtc aataaaagat aaaaaaatac ttgtggttga tgaccacaag gaattattaa
     8581 aaatgataga tgaaatcctg agaaaagagg ggtttagtag agtattttta gcatcaagtt
     8641 atgaggaagc tgttagggta tttagaaatg taaagccaga ttgtgcaatt ttagatgttg
     8701 tattgccaga tggagatggt ttttctatta tgagaaaaat tagagagaca tctaaaatac
     8761 cagtgatttt tctctctgca agaggtgaag atgaagatag attgattggt ttgggcttag
     8821 gggctgacga ttatatagta aagccatttt taccaaagga acttacttta aggcttgtgg
     8881 gtatattaaa tcgtgtatat gtgcctatag aagaagagga gttgccagta tttaaactgg
     8941 gagattctgt tgttgtgaat ttaaatagtg catgtgttga gaaagatggg caagagattt
     9001 ctttaacagc taaagagcat tcgttgcttt taaagttgta tgaaaataaa gggagaattg
     9061 ttactaatga ttcactatgt caagccattt ggggagatga tatgtatgga tatgaaaata
     9121 cccttatggt tcatgttaga agggttagag agaagataga aaaaactcca tctacaccta
     9181 aacatcttat tacagtaaga ggtcttggat ataagttgat gattaattaa ttttagatat
     9241 tagaataaaa gaaatcaaga ggagtgattt aattttggat agtgtaaagt acatattgag
     9301 gcgatttata ggttcaagta tcttgatttc attacttttt attgtattaa atatagcagg
     9361 ttttcttctg ttttcatata tgctatttgt atctaaatac tctgataatt tagatacaag
     9421 tgatagcaga gaaataaaat caaacttgat tatgattgaa gataatttga ttttaagaaa
     9481 tggaaagttt attttagatg attctagtaa ggaagtacta cataaaaata aagtttgggc
     9541 aatgttaatt gataacaatg gagatagaat atggggattt gatttaccaa aagaaattcc
     9601 aagtcattat actttgacag atgtagcaaa gttttcaagg ttctttttaa aggactatcc
     9661 tgtgtatgta tgggaacatc caaatggaat attagtaaca ggttatccaa aagacaagta
     9721 cactaagttt aaccttactt atacggttaa ggaaatagaa caatttccta tgttaataat
     9781 aggtatgttt ttggttaatg caatagttac ttttttaata gctctattca taggtttaaa
     9841 aatggtgaaa tctataaaac caattattac tggcataaag cttatgtcaa aaggagaacc
     9901 agtactttta caagaaaaag gaatactaag tgatatttca aaatctatta atactgtatc
     9961 taaagagttg caaatgaaag atgaaaaact taggaagaaa gaggaagcta ggtctaactg
    10021 gattgcagga atatctcatg atataagaac tccactttca atgataattg gatatgctgg
    10081 agaacttgaa gaaagcagta gcctttcaaa tagagaacaa gagcaagtgt ctattatatg
    10141 caatcaaggg attaaaatca gagaattagt aaatgaccta aatttagtgt ctaaacttga
    10201 atataatatg caagcattga attgtgataa gattagagtt tctgcgtttt tgagagagct
    10261 tatttctgag tttataaata ataatttgga taatgacttt tacgttgaat ttgatatact
    10321 aaatgaagat attattatag atgcagataa aaaattatta aaaagggcta ttagtaattt
    10381 aatacaaaat agtataactc ataaccaaca aggatgtaat ataagggtta cctctaagtc
    10441 tgatttagat ttttgctata taattgttga agatgatgga aaaggtatga gtcaagaaag
    10501 aattaatagt ttattaaaga gccaaaatgg actaagtgag gcatatgcca aaggtcaaaa
    10561 tcatggtcta ggtattttga tagtatctgg cattattaat gcacatagag gtaaattaga
    10621 tattacgagc attgatagtc agggactaaa aacaatacta aaattacctc taattaagtg
    10681 atattttata aaataataac agataagaat gaagaatgaa aatattttga aaataatttt
    10741 attctttatt gacaaattaa ctctattaga gtagaataag gataaattct tttcttgtca
    10801 agttttatta aagtaaagag taacttgtgc tttatgcgct tatggctgag ttactcatgg
    10861 tatcaaaatt ttcatagaaa taatattttg gaattattgt aaggttatta aattctttag
    10921 ataattccat tttttttatt ttttagttaa tatttaattt tattattgac aaaaaaattc
    10981 tactaaaata gaattatggt atagtgtttt ttaattttaa gaaaaaatat aatgacatcc
    11041 taaaaaagtt ttaattatac gctgttctgt gtagttataa tagcttctat gtaactattg
    11101 taagtatatt attatagtta tttatttaaa gtacgataaa tgttttaaat tgaagtattt
    11161 tgcgccattg agttctacta aagtagagtt tttgtgtagt atttttagaa ataacatata
    11221 tatatatgta tttatgttga aatatatggg aaatttataa tataattaat gaaaaaattt
    11281 aaactttgta atatataaat aaaaaaactc taaaatagaa ggatataatt atatagaatg
    11341 tttcaattaa acaaggcaaa aactataatc ttaaaattaa cattattctt aagaagtaag
    11401 aaatttaaga agtaaaaagt ttagttataa gtggtagtaa gcaagaaatt taagaagtaa
    11461 aaagtttagt tataagtggt agtaagcaag aaatttagga agtgggaagt ttagttataa
    11521 gtggtagtaa gtaagaaatt taggaagtgg gaagtttagt tataagtagt agtaagtaag
    11581 aaatttagga agtgggaagt ttagttataa gtagtagtaa gtaagaaatt taggaagtag
    11641 gaagtttagt aataatttgt agcaagtaag aaacttagga agtgggaagt ttagtagtaa
    11701 taatttatag caagtaagaa atttaggaag taggaagttt agtaataatt tgtagcaagt
    11761 aagaaattta gaaagtagga agtttagtaa taatttatag caagtaagaa atttagggag
    11821 taggaagttt agtaataatt tatagcaagt aagaaattta gggagtagga agtttagtaa
    11881 taatttgtat caagtaagaa atttagggag taggaagttt agtaataatt tatagcaagt
    11941 aagaaattta gaaagtagga agtttagtaa taatttatag caagtaagaa atttagaaag
    12001 taggaagttt agtagtaatt tatagtaagt aagaaattta gtaagtgaga agtttagtta
    12061 taatttatag taagtaagaa atttagtaag tgagaagttc agtaataagt tgtagtaaat
    12121 aaaaagttta ggaagtagga agttcagtaa taagttgtag taaataaaaa atttaggaag
    12181 taggatttac tgaaatacaa atatttaaaa tcaaagagtt taaaagaaag gagagctact
    12241 ggacaagtat ccaaaataga tagctgagta caaaatattt cataaaatta agcaatataa
    12301 tacaagagaa ttttgacatt atttatatat atttaccgtg tacaaatagc taaatttagg
    12361 attaagattg aaaaatatct tataagtctg agcttatata aatccgtata agatgtattt
    12421 aatcgaaaaa ttttattatg gatattgctt gtctatattc tgagtagcaa ggttttatca
    12481 tgactataaa aaagttgcca caatcagaat taaagataat gaaatttata tggaaatcag
    12541 attccaaagt aacatctaga gatattgttt taggaatgga gcaaaaatat caatggaaac
    12601 aaacgacaac tttgactctt ttatctagat tagtagtaaa aagatttcta aattctcaaa
    12661 agatagataa atatacgcat tatgaagttt taattaaaga aaaggaatac ataggagttg
    12721 agacaagaga tttttttaga aatattcatg attcttctat aaaaagttta ttgttgtcat
    12781 tacatgaaaa tattaatttg agcaaagatg atatcttact tattgaagag tggataaaaa
    12841 atctaaaaga agaggagaaa gacgtataag caaacgtatt tcttatagca tcaatataaa
    12901 gtgtatgtaa gtgtatttat gtaatatatt agtctactta agtaatatga caaatgatta
    12961 taagtggatt atgatactat aatgtgaatc taaataaatt aaataaaaat taatagtgga
    13021 tattctattt aattaaattt aaaaagctac ctttacttat aagtataatt aaggtagctt
    13081 tttaaataga ggtagtgtaa ttaaagagaa tttttaaata taaattgtga agctttaata
    13141 tagatgataa tatatatttt gcaaatatga cacagtgttt tcgtatttaa gatgataaaa
    13201 taaataggaa ttaatgatga tattgaaaaa ataaaaataa aggattaata taagatatta
    13261 aagagataga gataaaagtg ttaacagaag atattgaaaa aataaaagat tgatataaga
    13321 tactgaagag gtagaaacaa aagtattaat agaagatatt gaaaaaataa aaacaaaaga
    13381 ttgatagaaa gtactgaata gatagagata aaagtgttaa tagaagatat tgaaaaaata
    13441 aaagattgat ataagatact gaagaggtag aaacaaaagt gttaatagaa gatactaaaa
    13501 aagtaaaaat aaaggattaa taaaaggtac tgaagagata gagaaaaaaa tgttaataga
    13561 agatactgaa aaaatagaaa taaaaaatta ggaggaaaat ttatgaaata tcaggtttca
    13621 ttattagcag tgaaggatat tgaagtatca aaaaaatttt acaaagaatt atttgagcaa
    13681 gaaattgaat tggacttagg taaaaatgta acatttaaag gaggatttgc tatacaagaa
    13741 gacttcgcat ggcttacagg gattaatcca gatacaatta tacaaaaatc aaataatatg
    13801 gaactttatt ttgaggttga tgattttgac gaatttattg aaaaactcaa ttattataaa
    13861 gatgtagagt ttgtccataa gccactaaaa catgaatggc aacaaagagt agtgcgtatt
    13921 tatgacccgg ataagcatat tattgagata ggagagtcta tggaagttat agctagaaga
    13981 tatttatctg agggatactc tgtagaagaa acttctaaaa taattcaaca tccaattaaa
    14041 tttgtaaagc agtgtgaaaa tagtaaggaa tgattgttta aattataatt atttagtaga
    14101 aattaattgt aatagaacta attcaagata atatatagga ctagtatttg acaaatgtca
    14161 taaattaaat taaaataaag aaaatattaa taaagaaaaa ataaataaaa ataaggggaa
    14221 tattaataaa gaaaaaataa atataatatt aaaatatatt aataaatttg aatatttata
    14281 atatttttaa tttgaagtag gaatacttaa acgtataaag tttattatcg gaaatttcta
    14341 tattaatatg tagtaattca atattattat aaaggagtgg ttattattaa ggcaattgaa
    14401 ctttataaaa aattagattt agaatttaat attaaaaata ttaatgatga ttggagtttt
    14461 atgaattttg aaaatgattt tattactcca gaatttagaa aaaaatacat aggattagta
    14521 ttagataatg ctcaaaatat taataaagta tacacaacta cttttcctga taaagaaata
    14581 attaagcaag ttatagataa agatgaaaaa gatattttaa ttttttcaca tcatgcaatg
    14641 ggatacatag catcagatga aggttttcca tttcacgaca taccactatc ttatatggaa
    14701 gagatgaaaa atcgtagaat ttctttttat gtattacatt ctccactaga caactatagt
    14761 gactattcaa cttctgttag ttttgcaaaa gctatgggtt tagaaatagt caaaccattt
    14821 tgtaaatatg acgataaaat tgaagtagga gttatatgta aaacatcact aaaatcaata
    14881 gaagagatta aggaattaat taaaaaatct gttggtcatg atgtgaaatt gtatgattat
    14941 ggagattctg tattaaaaga tggtttagtt gctattgcag cagggggagg aagttatcca
    15001 tttgtagcaa gagaagttgc tgaacttggt ataaatctat atattactgg gtttactaag
    15061 cctttaaaac attttgaacc agtattagag ttccatcaaa tagcaaaaga taatttaatt
    15121 aatgttatag gtgctactca ttattctaca gaaaaatttg cgtgtatggg catggtcgat
    15181 tattttaaaa atctaggttt agaatcagaa tttttgcaag gaaagtatta cttagaggat
    15241 ttataggaca tattaaacaa taaaaataaa cagttagtaa aaatttattt tactaactgt
    15301 ttattttttt acctatttat ccagtgtttt gaaaaaatct atctttattt tttaaactaa
    15361 gaaaatagac ttttaattgc tgattagaag atttagtttt gatgtctttt aaattatttt
    15421 tcgatatttt ttagtttatt ttttgatgta ttttagatta ttatttagga ttgattagaa
    15481 tttataaata tttattttag attattataa atatttattt taaaaaaata gaattattat
    15541 atttatgata ataaatggta taataaaagg taaagtgtca aatatgcact aaattaaata
    15601 agttaattag tatataggtt aaagtggaag taattaaaac ttattaagta ttagaaaatg
    15661 acttcttatt agagtaagta tctaaaatga tataaaaatt atgattactc attaaagtga
    15721 ctattataga gtggataaga ttactcatta aagcgattac tgtggagtag ataaaattac
    15781 tcactaaaat aactatcata gagtggataa gattactcat taaagcgatt actgtagagt
    15841 agataaaatt actcactaaa ataattatca tagagtggat aagattactc attaaagcga
    15901 ttgctgtgga gtagataaaa ttactcacta aaataactat cacagtagat aagattattc
    15961 attaaagtta ctatcacaga gtggataaaa ttacacatta aaattgtctg ttaaagaatg
    16021 aataaaaaag agattgctca atataagaag catataaaaa ataaaaaatc aaacgtataa
    16081 aatttaataa ataataactg atagggaaag gaaagataga atgatattta caaaaagtaa
    16141 taaatacgat aaggactttc tcatgaaaaa tatgatggga cctaattgta taaaaatatt
    16201 ggaagaatta acaagtaaga taaaactaga aaagggaatg cgtattttag acttaggttg
    16261 tggtaaaggt atatcttcga tatttttagc aaaggaattt gatgctactg tatttgcaac
    16321 agatttatgg atagaaccaa ctgaaaacta taaaagattt aaagagttta aattggatga
    16381 taaaatattt ccaatacagg cagaggcaca tgaactacct tatgcagaag gtttctttga
    16441 tgcggtgata agtatcgatt cttatcacta ttttggaaac aaagaaggat ttttagacaa
    16501 tcatatatca cctcttgtaa aagagggggg aatacttgct atggcaatgc ctggattaaa
    16561 agaagatttc gtggattgta taccagatga actcattcca ttttggcaag ataatatgaa
    16621 cttccattca attacatggt ggaataaact ttggtctgaa tcggagtctg tgattgtaga
    16681 gaaatgtgaa gctttaaatt gtcatgatga agcttggaag gattggataa attgtgataa
    16741 ttcatatgct ataaatgaca aaaaaatgat ggaagtagag aatggaaagt attttaatac
    16801 aatttctttg atagcgagaa ctaaataatt gtgatgaatt tataaaagta taagataagt
    16861 agaaatataa gttgaatgag agtataagaa gcatagaaat ataagttgaa ttaaagtaga
    16921 aggtaaataa aaatgtaagt taaataaaga gtttatacaa aaatgatttt ttagattatt
    16981 tatacccaat cgagttgctt aggtcagttg ggtattttta ttttttaaag ttaaattgca
    17041 aatactattg tgtgatatat attttaatac taaatgtttg ttttataaga aataaactaa
    17101 tagaaaaaat agttttaaca atgaaagcaa aaaaggtatt gatgaagatt aaaaatttta
    17161 tagtaccatt ttatatattt tattatactt taaatcaaat ttcaaaatga ttaagtatat
    17221 atacccaaaa gattaaagaa ctatatttat tatactatta aaaattattt taatagagat
    17281 acaagagata gatatattgg ttttaatttt atagaaatat acaaagatat tttaagctaa
    17341 tatatagtaa tttatatagc attataaaat agaatcatat aaaataagtt aactacaatc
    17401 aaaaattaaa aaggggaaat gttaagttga tagaaggcat aaaatttata aaggcagaag
    17461 aaaagtatat ttattcgtat tggcaaactt ttgataaaat agcaaaagag aggaaatatc
    17521 tagctatgga tgaagccttt ccttttgaag aaacagttga atttataaaa aatattataa
    17581 ataaaaactt accacaactg tttataattg atttagaatc agataactgt attggttggt
    17641 gtgacgtttt acctaaaact gaaaaagttg gttatttagg catgggtatt cttaaagagt
    17701 atagggaaaa aggtatcgga agtaatcttt taaaacaaat tattgattta tcaaaagaat
    17761 atgggtatga aaaaattgag cttgatgtat ttaaaagtaa tagtagagct attcatgttt
    17821 ataaatcttt agggtttgta gaggtaaata caatatctag tggttttaca tggaatgata
    17881 gacctgtaaa agaagaagta attcaaatgg agcttacact tatataaaat aaagttaaag
    17941 tctttaaaat tccatataca cttgtataaa ataaagttaa agcccttaaa attccatata
    18001 aaagaaatat ttttaagggc tttttaataa tgcttctttt gaaggtagct aaatatctaa
    18061 aattttatat attaaattta aagaaaatat aaagatggaa gatacaacca tgtttactaa
    18121 taaaaaaatt aaagtcataa ataataaact cataaacaac ttattattac ttatgtttat
    18181 tttgcatcta ttacttcatg agccatttta actaagtcat tcatagataa atttgagtct
    18241 tgaactgtaa aagaatagta aactccatct tgtttccatg tcaaaaactt aaatttatta
    18301 acttcaactt tgtctgattc attatttggc cctacactaa atgtgtattt gcctgatttt
    18361 tcatcaattt tatcctgttc tgtcatttta taaccttctg gtacaaactt ttgtgcgaat
    18421 gcttcataag aaatattaat atctttgtaa gtatctacaa cttttagatt gtcagaactc
    18481 ttcccaagta cattatttgc tgtgtcaaga gatatttcat cattgccatt tttatattca
    18541 aaactaagtg cttttgattt tcctaaaaca ttattttctt tgtcaaatgc ttttttattt
    18601 acagtatatg ctcctttaaa tttatatcca ttactaaatt cagaaactat attaggggta
    18661 aagttaaagt ctttttttac ttgttctgta gttgggatag atgtataggt agccttagta
    18721 cttgattttc caaccaaact atatatatgt cctactgcaa gggctgttgt gctcaatatc
    18781 aaagtaatgg ctaaagttgc aactaagcct tttctaaatg gtaatttgtg tgttttatta
    18841 ttaatcattt tataattttc ctctctttct ggtaaactat ttagtgtttt gcttaaacta
    18901 ttatgaaaac tttctggaac ttctggaaac gaattattta atatattttt attcatatga
    18961 ttacctccgt gttttctaat ttagttttta ataatcttct tcctcttgat aatctacttt
    19021 ttacagttcc ttgaggtata tctaatattt ccttaacttc atctactgaa taaccttcaa
    19081 tatagtaaag gactattgga attttaattt ttttaggtag actttgaata gcagtataca
    19141 aatcgctata gtcatctttt tcatcaattt ttttgttttt gaagacatct tcaaatgata
    19201 gagattttaa tcgtttgcgt cttaatgaat tacattcatt tatgactatt ctaatcagcc
    19261 atgttttaaa gtattgttct tccttaagag aatccaactt ttcatatgct tttaaaatag
    19321 cattgttgac ggcatcttca cagtcttggt cgtttcctag aatagactta gatactcgat
    19381 acaaagtctg ttctgattca agtatattgt ttgtaaatgt agttttatcc aactaagttc
    19441 cttcccttct atgcaaatcg tagcgctact tttttgtctt tcacttatta gataaaattt
    19501 tactatagat ggttcacttt ttttatgatt atttgataaa tttaagtttg aaagttttgc
    19561 ctattctata caaatattct ataatatatt ttttctagta ttttcccctc atatataaag
    19621 ccatgtagat atattaaact ttagaagcca tttatatatt tagttttata gtaagtcttt
    19681 atattgagta attagaaaat agtaagtatt taatgttgag tagttagaat aacaatgctt
    19741 ttggattatt tacttcatgt acagatttaa aataagttct ttaaatagca actttttgaa
    19801 aaaacgcaaa aatattttat gttgtctgat tatatttata gatgtgaaac aatattgtat
    19861 agagtaaaag tatatagaag atgattacat agagtttata cttgaatttt acaaatataa
    19921 ttatcaaaat actgtcaaag agaaaaatag ttttgatgaa cgtttttatc aaattgctac
    19981 agagacaggg ctaagtgcta atactgtata taaagtattc aataaaataa taaatagtgg
    20041 aaaaactgaa tttacatcaa aggaattagc tactatatgt ggtgtaagta ttagaactat
    20101 ggacaggatt atacttagat tgtgtgattc tggttattgt gaggtagtga gtgaacagct
    20161 tatgagcaag tatggtagac ccagtagaat attacgtttt aaaccttttc tgttgtttta
    20221 aatagatata agtcaaaaat taaattttta tgataacatt ttctttacaa ttttttagat
    20281 atatgttaat atttagaaaa atgcatatgt attcataaaa taaacttaaa acagctaaaa
    20341 aatcattaaa aaggaaattg tatgcatgaa atgagaaagt taatttataa cttaaataaa
    20401 tctgggagga gataattatg ggaaaatatc aggattcaaa aaaagttgta agaaattatt
    20461 ttgaagcatt agaaaatgct actggggatg aggttgaaaa tgtcttaaag caacatatga
    20521 aatcagatta taactggaaa ggtgtgtatc catttcgtga acaagaaggc actgaaaatg
    20581 tagcagatgt attttggaag cctttaaaaa aatcattgag taatatgcaa agacgacaag
    20641 atattttcat tgcaggaaca aatgagattg acaatagtga atgggtaatg agtatggggc
    20701 attttatggg actttttgat aacgattggc ttggcattaa acatactcgt aaaatgatta
    20761 gtttaagata tgcagaattt aactgtgttc aagatggtaa aattgctaaa actggattat
    20821 ttgtagatat aattgggttt atgattcaag caggagttaa tccattgcca ccacaaacag
    20881 gttcatattt tgtatatcca gggccaattg accataatgg attacttttt gaagatgcag
    20941 atgaagttga aggagtaaaa actttagcat tagtaaataa aatggtagat gatttatctg
    21001 aattaaatga aagtggtgct atgggatgcc caccagaaat acttgaaaaa tcttgggcta
    21061 aagatatgat ttggtatggt ccaggtggaa ttggtgcttc ttatactata cctagatatc
    21121 aagagcaaca ccagctacca tttagaaata atttaaaagg aaagactttc aatgggcatg
    21181 tatgtagatt tgcagaaggt aattttgctt gtttctttgg atggcctaat ctgactaatg
    21241 taccatgtgg aggtttttta ggattaccag gtggagaggt taaagcagat atgcaagttg
    21301 ttgatgttta ttatcgtaag ggcgataaac ttcaagaaaa ctgggtgtta attgatattc
    21361 cttattggtt gaaacaacaa gggcttgata ttcttgagag aactaaaagt atttttaatg
    21421 cttagttatg catacgtatt ctaaaataaa gggggatata aagtggcatc taccaacaaa
    21481 aataatgcaa caagtaaaga agaagaaaaa gaagtttcta caagtgggct tgaatcatgt
    21541 acggttaaag aaaacaaagc taaaatgaat caaactaatt ttagtaatat aaatgaaaat
    21601 caaacaaatg agaataaaac gaacacatat aaaagaaata tagataaagg aaatatagat
    21661 aaagggaata tagataaaac aaacacaaat aaatccgaca taaataaagt taacatagat
    21721 acagtggata cagataaatt aaatacaaat actcttacta aaaaaaataa aaaagttata
    21781 tttaaggggg aagataatat gtcatctaca aaaatttact atggagattc attagaagta
    21841 acgcctgttg gagtaatgga ttacaatgat ttttcaaaac agacaaaaag ggaacaggaa
    21901 atacctggat ttgattctaa gtatagagat tttgtagatt atattatgaa gattacacat
    21961 aatatctggg aagaaaaagg tataggtgtt atttatgata catatcacaa taatgttaca
    22021 atgcattgtg gctcttcaaa tttagttggt attaaagatg ttatttccaa tactcttcaa
    22081 acattacatg cgtttcctga tagaagatta attggtcaaa atgtaatatg gtctaacttt
    22141 ggtgctgatg gttttttatc ttctcaccgt gttttgtcta cagcaactaa cttaggtgat
    22201 agtaattttg gaccagcaac aggtagaaaa ataaacttta gaactgtaat tgattgtgcc
    22261 acaactaaca atagaatcca cgaagaatgg ttggttagag ataatttatg gattgttact
    22321 caattaggat taaaccctca ggaagtagca aagagtatgg caaaggcaag tgaatctaaa
    22381 gtgcttagtt tacaatcaac ttatggtatc tgtgaatcta tggatggtca atttatgcca
    22441 actaagtatc aagcaaaaga tgattctgct ggcgaaatga tgcttgaaat gacaagccgt
    22501 atttataatt ataaatatat aaatgaagtt aaaaaatact atcatgataa tgcagtagta
    22561 cattttatat gtgataaaga tttaaatggt tatgatgaga ttcaaggaat gattgtaagt
    22621 ttacttgcat caattccaaa tggaagttat gaagtggaaa gagtaacttg taatcaaaga
    22681 gaaaaaaatg aaggttatga tgtatctgta agatggagat tacgtggaat aaatgaaggt
    22741 ataggatttt ttggacagcc ttctggaaag catatagaag taatgggaat caatcattat
    22801 catatactac aaggtaaagt aaaggaggag tggataactt ttgatggtat ggatgtatta
    22861 aaacaaatgt atatgggagt agaagaataa agctgtttag agtataaata gttttgaaat
    22921 atatagggaa gtattgcttt ataaaattta agtggggaat gtatatgaaa aatagatgtt
    22981 taaactttta gcaaaattat gactaaattt aaaaggggga attataaaaa tatgaaaaca
    23041 acggttgaaa tagaaaatga tatcatatta ggaaaagact attcaaacct aaaaagagga
    23101 attgcatttt tttcaattgc tttaatatac tttttttatt gctacaattt tatggtgggt
    23161 acttttatta aaccaacaat gatttatgct ttagcagatg ggggatttgg tttttcatta
    23221 aaacaaacag aagaaatatt tgcagttatg tcttttggta caattccagg tacatttatt
    23281 tttggggtga tttctacaaa aattgggaag aagaggactt taattagcgt tgctttactg
    23341 attggattga caacatttat accaatgtta tctcctacaa atattatgtt atggaaaata
    23401 gcacgtttgt gtacaggagt agtgttggga ggcgtatttg gaacagctat gcctttagtt
    23461 gcagatatgt ttccaagtaa atatcgtggt aagttagctg ctatattaac tgctttattt
    23521 tcattagcaa tgatttttgg tgggaaagta tatggcgttt taggtgatgc aaattggcaa
    23581 atattgatgt atactgcgat tataccacct atagttggag ctatcttagc aattttcttt
    23641 gtgccagatg atttggagta tacaaaggaa ttagttaaaa agggaaaaga aactggtgaa
    23701 aagattagct atataagtat gtataaggga aaatacttat ggattggatt gggaacgatt
    23761 cttttatcag gtgcaaattt tacagcatat tcagcatttt caaataatgc aactacatac
    23821 ctagtgacaa gaataggaat gtcagcagca gtggctgggt ctatatatag tttacaagga
    23881 attggtcaat tagtaggata tttattctgg ggttcaattg cagataaatt tggaagaaaa
    23941 atacctttta taggtatggc attagcagca gtatttgttt ttatatatac aaaattaggt
    24001 ggagataata caacaacatt ctatatgata tcagtattac taggtgttgc tgttggatat
    24061 tctggagcat ggggagcata ctacacagag ttgttcccaa gtaaatatcg ttcgttgtca
    24121 tctggtatgt catttaatgg aggaaggatt atatctacgt ttgcaatacc tgctatagct
    24181 ggtacagctt ctggtacttt aggtatgatg actatctttt acatatcaat cgcagtcttt
    24241 gtagcaggtg ctattgtatg gttattctta ccggagacgt taaataataa aaaataaaat
    24301 actaggaatt tattttgtta cttagaaagg ggtggcttat gatttcatca attgatgtat
    24361 gtattataat aggatatttg ctattaatgt tagttattgg ttattattca ggaaaagata
    24421 ataaaaatca agaagattat ttcttggcag gtaggtcaat gccatggatt ccaattgctt
    24481 tatcagttgc agctactatg attagtgcaa atggctttat aggtggtcct ggatgggcat
    24541 atgtagatgg aatgtatcca gtaatggtta atattacagt acctttagca atattttttg
    24601 cactgagcat taccacccca gttatctata aattaaaaat aacctctata tatgaatata
    24661 tgggacttcg tttaggaaat tattcaagag gattaactat tattcaattt tttattaatt
    24721 cattgattca agctagttct atggtatata ttccagtgct tattattcaa atgattactg
    24781 gctggtcact ttatgcttta gtcccaatag ttgtacttgt atcaattata tatactctat
    24841 taggtgggat taaggcagtt atatggactg atagtattca aatgatagta gtaataagtg
    24901 ctgtatttat agtaattatt gtagccttaa aaggaatgaa tctaagtttc tttgatacat
    24961 tacaaattgc acaacagagt ggaaaattaa atacatttga tttcagtagg gatatttcag
    25021 tgacaaatac tttttgggct actttaattg gtggaactgt aatgtggata agatactttt
    25081 gttttgacca atctcaagta caaagagttt taacatctaa atctttaaaa tgtgcaaaaa
    25141 attcttttgt agttagtgca tttgttatga atttagttta ctactttatg ctattggtag
    25201 gagtaatttt atttgttttt tatgggggaa ggacatttga aacttcaaat gaaataatga
    25261 ttacttttat tttaaatgaa cttccagtag gagccattgg aattattatt gcaggtgtgt
    25321 ttgcagcagc aatgtctagt atcgactcta tattaaacag tatgacaact gtttttacga
    25381 aggatattta tgagttttat ttcaaaaaag acaacaaaga atcatcatta aaagttacaa
    25441 tgattataac tgtagttata ggagttgtta tgacaggttt tattattatg ggatttggag
    25501 gaagcatcaa atcaattctt gaccttgttg gtaactatat ctcatacttt gctggaccag
    25561 ctacaggtgc atttgtacta gcaatgttta catataaagc taatgataaa ggagtatcta
    25621 ttggttttat tgtagggtta gttttaggat tttttatttc taataaatat aatgtgagtt
    25681 ggttgtggaa tccagcaata ggtgctacaa taacattgat atttggatat atttttagtg
    25741 tcattttaaa gaaaaatcat aatgcagatg atattaaaaa atacactgca tttggtatga
    25801 gaaatcacct tatttctcaa ggaatggata aagtagatgg agtatctata gtgccatttt
    25861 ctttaggtaa acaagaacta attgtattag ctttttttat tatccaatat attatattat
    25921 ttttgataca attttaagaa gaaatactag atatttaagg agaaatacta aatgaatcaa
    25981 aagaaagttc attataataa atcggctacc tcaaaagatg ttgcaagatt ggcaggagta
    26041 tctcaatcat ctgtttcaag agcatttggt agtgcaaatg gaaagggcgt aaaacctgaa
    26101 gtaagagaaa gaatttttcg tgtagcaaac gaaataggat atgttcctaa tttggtagca
    26161 cggggaatga ttagtggaaa aactaatgtt ataggattga tagttggtga tagtctaggt
    26221 ccattttaca atagaattat caatttattt gtagaaaaaa ttcaagagat aggtaaacag
    26281 tgtctcgttt ttaaagttcc tagacaacaa agtatagata gtataatttc aaaagtaatt
    26341 cagtttcaag tagaagctgt gattattaca gcttcggcca tgaataaggt gatggctgag
    26401 acttgtgaag aaaacaatat tcctgtgatt ctttttaatc gttttatacc tggtataaaa
    26461 ataagtacag tctatgtaga cccaattgaa ggtgggggaa tggctgctga atatctgctt
    26521 aagaaaggtc ataagaatat aggatatatt caatttacga gagaaacaag tgaagaaatg
    26581 gaaaagaaaa ttggatttta ttctaagtta agacaaagtg ggattcataa ccttaaagaa
    26641 gaatctgcaa actatgatta tgattctgga tatgaagcag gtaagagaat gttagcatta
    26701 tcaaatcctc caacagcaat tttttgcaca agtgacttga tagctattgg tgtaatggat
    26761 gctgcaaggt ttgagtacaa gttgagggtg ccagaggatt tatcagtgat aggatatgat
    26821 gatattcaaa tggcttcatg gaagagttat aatttaacga cgattagaca gccactagat
    26881 ttgttgattg aaaaaacaat taacatactt aagtccttat taaatgaaga aaatccagag
    26941 tcagttgttg aaatgttaaa acctgaattg attgaaagag gttctacact ttaagtggta
    27001 gatatttaat taagtggtag atacctaatt aagttgtaga tatctgataa gaaattttta
    27061 agttttcaat cgttctaaat tgtgagtata aaaatctact aaatttagtt atgtaaaata
    27121 taaattataa gtgtagaaac taggctacta gcttttatac cacttcaaac attactggag
    27181 gtagttaagt ttacatcagt ttagttttat gtagatataa tattctgtaa atagacaaaa
    27241 ttaaaatatg taagtgtctc aaaatgaaat ttttagttta tgaggcactt ttttgtataa
    27301 aatcaaatgt atttttataa ctttaacaat aaaaagaggc ttatctctat tttgagacag
    27361 cctcaaattt ttaaaactat attagtttac agcttctttt cctatcaaat cttttaactc
    27421 ttcatgagtc atagtttctt tttcaaataa gaatttagaa acagaatgta aatcttcaag
    27481 attatccttt aacatctcta aagtctcttt ataacaaatt tcaactatac gatttgcttc
    27541 cttttgaacc ttatcacata agaaagtctt atcatttcca tcaatagtca taaatcctaa
    27601 gttactcata ccatattcac aaaccatttg gttagctatt tcagtgactt tctttaagtc
    27661 gtcctttgca ccagtagatt tatgattaaa tattaattct tctgaagcct ttccagcaag
    27721 taatattttt atttttccaa tcaactcatc cttagtataa atatatctat cttcatcagg
    27781 taattgaaga acataaccta atgcttgtcc tctaggaact atagatattt tttgtatagg
    27841 acaaattcct actatatcac ttacaagagc atgtccagct tcatgatatg ctactatttt
    27901 tttctctttt tcaaccaatg cagagttttt agattctagc cctgcaataa ctctttcaag
    27961 tgccttgtca aaatgttctg atgtaataca ctcactgtca tctctaactg caaatatagc
    28021 tgcttcattt gctatatttg aaagatgagc cccattgaaa ccatgagttt ttttagctaa
    28081 aagttctaga tttacagatt tgtctatagg tttgtcatct gtatgtacct ttagaatttc
    28141 aaatctagtg tgatagtttg gtgcacctat atgaatgtgt ctgtcaaatc ttcctggtct
    28201 aagtaaagct tcatctaata agtcaagtct attggtagca cctattatta atacattgga
    28261 atctttatta aatccatcca tttcaactaa aagttggttt agtgtttggt ctttttcgtt
    28321 gttactttca agatgtctct ttgctcctac agcatctatt tcgtctataa atattatact
    28381 tggagcttct tttctagctt tttcaaatag agttctaact ctttttgctc ccacacctac
    28441 atatttttca acgaactctg aaccagtaac attgaaaaat gatgagtttg tttcaccagc
    28501 tacagcagat gcaagtaggg ttttacctgt tcctggagga ccataaaata atactccttt
    28561 aggtattttt gcacccattt tttgatattt ttgtggtgac ttcataaaat cgactatttc
    28621 aaatagttct tcttttactt catctaatcc agcaacatct ctaaatgtag tctttggttt
    28681 tgtagaaagt gaatcatctg tttgattatc tctttccatt tttgtctgtg caaagacagg
    28741 agctggattg ttgattttct taaaaaagtt gtttagcatt ttcatattca cacctcaggt
    28801 aattaattat tgttaatcaa ttttatattt tccatattta ctatttttta ttatttattt
    28861 ttcatggaaa aaatatacat gttatctaaa taaaaataaa tttgtaagat gtatgaaaaa
    28921 atgaatttat gtatatagag atttctactt atgtattgaa taaagtatta caaattataa
    28981 attatgtatt gattatatat tgagtaaagt attataaagc atgtattgat taagtattga
    29041 ataaaatatt acaaatttag ttgaaatgaa aatgataaaa aatcttgtac tatattttac
    29101 aaatcatata aatagagaaa ttgttgtagc acaaagtagt aggtagtata ttagaatata
    29161 atataggatt tgttgtgtag tatagtagca tattagaata taatatagga tttgttatgt
    29221 agtatagtag tatattagaa tacaatatag aatttgttgg gtagtatagt gttatattag
    29281 aatacaatat aggatttgtt atgtagaata tatagagtca tgtagaatat aaatatataa
    29341 aaggattttg ggtataaatt taaaaattaa tagcaaaatg agtagtatag acatataatt
    29401 agtagtagga agtaattaaa tttttcctaa acaaaaaaaa tagttgcaaa taatttgatt
    29461 aagtgatata gtagataagg taagaataaa acaaagaaga agtccgcttc tcaccttacg
    29521 ccggaggtta gtaaggtaaa tataagttaa agcttaattt taatttaagt ttgtttaaat
    29581 ttggtaactt atattgcgga tgattacgtc atccgctttt tattttataa aaattttgga
    29641 ggtgtcctac aattagcaaa gaattagcaa tcaatgaaca aataaaagat aaggaaataa
    29701 gagtcctttc accaacaggt gaacaacttg gagtaatgcc tactaaagaa gctcaagcaa
    29761 tggctaatag caaaaactta gatttggttc aaatctcgcc gaatgcaaat ccaccagtat
    29821 gtaagataat ggattatgga aagtttagat atgagcaagc gagaaaagag aaagaagcta
    29881 agaaaaaaca aaaaactata gttgtaaaag aagtaagact tagaccaggt attgaacaaa
    29941 atgatttaaa tacgaaagct aataatgcta taaagttcct taaaaaaggg gataaagtca
    30001 aagttgaact tagatttaga ggaagagaat taggacataa agacataggt aaagaagtta
    30061 tgcttaagtt tttagatata ataaaagagt ttggagaacc aacaaaagcc cctgcatttg
    30121 agggtaataa tatggttgta attatagatc caaaaaaata gatgattaac tgagaggagg
    30181 aatttgtcat gcctaaaatg aaaactcata gaggtgcagc aaagagatta aagaaaacag
    30241 gaactggaaa gttaaagaga gctaaagctt ttaaaaaaca tatattaact aaaaaatctg
    30301 caaaaactaa aatgaactta agaaaatcaa ctttagtaag tgatggagat gctaagagaa
    30361 tagcacaatt attaccatac taagatacag attgtaaata ataactatag aagagggata
    30421 taaaaggagg tcataacgat ggcaagagtt aaaaaagcta tgaacgctcg taaaaagcat
    30481 aagaaaatat taaaacttgc aaaaggattc agaggatcta gaagcaaatt atatagacca
    30541 gcaaatacat tcgtaatgaa agcattaaaa aatgcttata taggaagaaa gttaaagaag
    30601 agagacttca gaaaactttg gatacaaaga ataaatgcag ctgctagaat gaatggaata
    30661 tcttattcaa gattaatgaa tggattaaaa ttatctggta ttgaagttaa cagaaaaatg
    30721 ttatctgaaa tggctataca agacccagaa ggatttgcaa aattagcaga agttgctaaa
    30781 gcaaaattag cttagtattg aatcatgaga tgtaaagtct ttgagttata taaataattc
    30841 aaggactttt ttattttttg aaaaatactt attagtgtca tgtacaacca attttataat
    30901 gataaataaa ataagcaata caatagaatt ttatgagatt tattataaga attgttacaa
    30961 ttatttatga tagctataaa aagtcatagt aaaaatgata ttgatgtatg ttgatatatg
    31021 tataaagctt tcaatcaatc ttgaattaat attatcaaca tgataatatt atcttattaa
    31081 taatataaat aagggggaga taatatgaat gaaaaactaa tggattgttt tgtaaaccca
    31141 attaaatgca agttattgat tgaaattttt tcaaataatc gaactacagc taaacaatta
    31201 ggagaaaaat ttcaagatat atcaaaggca acactttatc gttaccttaa taaaatgttg
    31261 aaagatgata ttataaaagt agttgaagaa aataaaataa ggggaactgt tgaaaaagta
    31321 tatgctatga agttagatat agctgatgat gtgaacaaaa ttatagatga gaactctgga
    31381 gaagaatatg ctcaactgtt tactcaatat atgatgggat ttttaagaga attttatgaa
    31441 tacgctgaaa gaaaggaaaa ggatatccta aaagatggtt caggattttc tgtctgtcca
    31501 gtatatgcta gtattgatga gatagttgaa gtgagtaaaa aaattgctag tatactagaa
    31561 cctctaagaa ataactcgca cgaagaaggc agaagaatgc attctatagg agtgataatc
    31621 actccaccta aagaggatga agggaaataa atctgattta catgaggtag agccaattta
    31681 cacggaaaat cgttttatga tttacatatg tatccagagc aaagtatctc tgcttggatt
    31741 gtacttcatt gtatcatttc atctatctaa tgacttggag tggtacaatg aagtttttta
    31801 atttttcata tcaatgccta aagaaatgaa ctttagacca catatgcaat taggttacat
    31861 tatacaatta gaccagatat acaaaataaa aatcagtatt tttatagcag ataaattgac
    31921 tgttattctt cttaaaaatc atctattagt tattgttagc atattgaaag aaattatagg
    31981 aatctattga ataaatgaat attgcccata atcatataaa ctgctatagt ttaaaataaa
    32041 gcaggacgtt aaattttata gtaatctaaa aatttaagag ggggatttta tgaaaataaa
    32101 atttgttatt atgccattgg tgatattttt aatggtatta tttgtggggt gtcaaagtac
    32161 aaaagataca gaagttaaag caaaatatac agaaattaaa atattaggta caggtgattt
    32221 gcattcaatt ttgacagact ctatggtttc atatgtaaat gaggaaaggg aaaagaataa
    32281 aaatctgctt atggtagatg cgggagattt ctatggtaca caaagtagag aaatgtggga
    32341 atggtcttct ggaaaaaagt taattaatat taaaagaaat ggtagagcag agtatgatga
    32401 cataattaag agtagtaaag atgaagtacc tatagttaaa gatatggcaa agttgaaata
    32461 tgatgctgta gttttgggag ataatgaatt tgtttcaaat gataagcaaa gtcttgataa
    32521 actagtttct gattttaaaa ataataatat gccattggta tcagcaaata tatatgaaca
    32581 atctggagaa aattatgtac aaccatatgt aatgaaaaat attaagacag atgagggcaa
    32641 tgtaaaagtt ggaatactag gtcttactat aaaagaagta ggggaaaccc ttggtcttga
    32701 ggattttgaa aaggataaaa aagctagaga gcttggagaa caagctgatt acaaaggaaa
    32761 attatatgca aatgatttag tagaagatgc aaagaaatgg ataaaagtta tggaaaaaga
    32821 aagtccagac ataatagttg cagttgtaca ctcaggggaa gaacctaaaa cagatagaaa
    32881 tccaggaaat agaataaaag agttagcaac tacagtggat ggaatagatg caattgtagc
    32941 tggtcatact catgaaaaaa tagatgaaca caaatataaa aataaatctg gagatgaagt
    33001 aattgttaca caaccaggtt ggcatggaga tagagtatca gagattaatt tcaaattaaa
    33061 taaagagaat ggaaagtggg acataaagga aaagtctagt aaattaaaaa taattgataa
    33121 agaagtaagt atagttgcta ctgcagattt acattcacat ttctctgata aattaacagt
    33181 tgaccttgtt aatgaacgta gtaacccaat tgaacctgtt gtagtagatg caggcgactt
    33241 cttagaccct caaacagatg aaatgactca atggtataaa gagtggaaaa gtataagaga
    33301 taataatagt gataaaacga ttagtagatg cccaatggtc atggatatga atcagggaat
    33361 gtatgatgca gtagtacttg gaaatcatga atttgtatcc gaaaaagatg aattttggtc
    33421 agatgaacgc cttttagatg ctgttataga ggattttgaa caggtggcaa tttctgtgtt
    33481 atcggcaaat atatataggc aatctggtaa aaattatgtt aaaccatata ttataaaaga
    33541 tgtggagacg agtgaaggta atgtaaaagt aggaatattg ggacttacta taaaagaagt
    33601 aggtaaaggt cttaaaaatg taaacttaca agagcaattt caatataagg ataaattgta
    33661 tgcaaatgat ttagtggaag atgcaaagaa atgggtaaaa gttatgaaaa agaaaaaacc
    33721 agatataata gttgcagttg tacattcagg agaagaacca aaaaatccaa aacatccagg
    33781 aaatagaata aaagaactag caactacagt agatggaata gatgcaattg ttgctagtca
    33841 tactcatgaa aaaatagatg agcatgaata taaaaataag tctggagaaa aagttatagt
    33901 gacacaacca ggtgagcatg gtaaatatta ttctaaaatt acctttaaag taaataaaga
    33961 aaagggttcg tggattatta atgataaatt cagtaaaaca attgaggttg aataacttta
    34021 taaaatttat agataaagta aattatctga tattgacatg atatataaga taaagtttct
    34081 aatattagta taatgcataa tgtgaaatat tttaataata agagaataaa caagacaaat
    34141 aaaaatattt taacatgtaa gatatagtat gaactattat actaatgctt actttataat
    34201 ttgaacatgt tagaataagt cttatttgaa aattttagtg tattagagta agtattattt
    34261 taccagtaaa aaatattgag aactatatgt agatttgctt aaaaaggaat ttacatatag
    34321 ttcttttcgt ataataaata gtacaatctt atatggtaaa gataaagtgt gatattaaat
    34381 tttattatca tccaaaatct aaggaggagt ctcttatgaa aataaaattt attatagtag
    34441 catcaataat atttttaata gtattacttg tggggtgtca aagtactaaa gacacagaaa
    34501 tcaatgtact cgccacaagt gatttacatt caattattcc agataattta atttcctatg
    34561 tagaagagga gagaaaatat gatgagaatc tacttatggt agatgcagga gatttttttg
    34621 acatgcaaag tggtgagatg aataagtggt ttactggtca aaaattagta agatttaaag
    34681 atgggatacc agagtacaaa aaaatagctg aaccacgtga aggagaagtt ccattggcta
    34741 aaaaaatggc aaagctgaag tatgatgcag ttacatttgg gaatcatgaa tttgtatcta
    34801 atgacaagca gagccttgat aaattggttt ctgattttaa aaataacaat ataccattag
    34861 tatcagcaaa cgtatatgaa caatctggag aaaactacgt aaagccatat gtaatgaaaa
    34921 atataaatac tgacaaggga actgtaaagg ttggtatact tggtctgact ataaaagaag
    34981 taggggaacg ttcaaggttt aaaagttttg aacaagataa aaaggctaga gaacttgggg
    35041 aacaacctca atacaaaggg aaattatatg caaatgattt agtagaagat gcaaataagt
    35101 gggtaaaggt tatggaaaaa aaagaaaagc cagatataat agttgccatt gtacactcag
    35161 gtgaagagcc aaaaaatcct aaaaatccag gaaatagaat aaaagaacta gcaacaactg
    35221 tagagggaat agatgctatt gtagcaggtc atacacatga aaaaatagaa cagcatacat
    35281 ataaaaataa ttctggtgaa gaagtaatag taacacagcc tggagctcat ggtaatagta
    35341 tatcaaagat taattttaag ttggataaga agaatgataa gtggtttata aaagacaaat
    35401 atagtgaatt aaagattttt ggaaaggaag taaatataat tacaacttct agtctgaatc
    35461 caaaattttc agatgaaatg gcagttaatt tgtttaatga aagagataga gatgttcaac
    35521 ctatatttat agatactgga gattttttaa atactaatac aaaagaaatg actaagtggg
    35581 ctaaagagtg gcaatacaat gaaaataaag gcatctataa aaaaataaat gaatggccaa
    35641 ttgtatatct tggttatgat gctgtagtac ttggtagcca tgaatttaca gaggataaag
    35701 agacttttga ggaaaaaaaa tctacattgg attatattat agaaaacttt gaagaaatga
    35761 agataccagt attatcagct aatatatatg atgaatctgg agaaaattat gtaaaacctt
    35821 atataattca taatgtagaa actagggaag gaaatataaa ggttggagta ttaggtctta
    35881 ctactactga taaggatgaa gaagacttaa gtagctcaag cttaagcaaa gactcaaaga
    35941 cttcgaaaat tcctgaagaa acaggaaagt tatatactaa taatctagta aaagatgcaa
    36001 gcgaatgggt aaaagttatg caaaaggaaa gtcctgatgt gattgttgca gttgtacatt
    36061 cagataataa tagtactcta aaaaatccaa gtagtgaagt caaaaaactt gcaatgtcag
    36121 tagatggaat agatgctatt gttgtaggtc atacgaaaaa taaggtagaa gaacatattt
    36181 acaaaaataa gtctggggat gaggttatag taacacaatc tggagaagat tgttattcaa
    36241 aaataagtct tgaactaaga aaagaaaatg gcaagtggaa tatcataaac aaatatagta
    36301 aagcaataaa aatgaagtga atttattgat atataaacca ttattaacac tgatttttat
    36361 aatataataa atactgattc tataatataa ttaacactac tttttataat gtcatcaaca
    36421 ctgattctat aatataatta atactacttt tttaaatata atcaatactg attccataat
    36481 ataattaaca ctactttttt aaatataatc aatactgatt ctacaatatt attaatacta
    36541 ctttttataa tataatcaac actacttttt a
//
LOCUS       DAEPIN010000040        35928 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.040, whole genome shotgun sequence.
ACCESSION   DAEPIN010000040 DAEPIN010000000
VERSION     DAEPIN010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 35928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35928
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.040"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            complement(<1..674)
                     /locus_tag="KR250_002143"
     CDS             complement(<1..674)
                     /locus_tag="KR250_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HBH0068077.1"
                     /translation="MAIIKKILNLEINDDYIRLVFLRKSHKKVFIDKSIIKEINFDIK
                     NDSNSIEKIKYIEDVVRFVKEEIGKSKILYKNLYFNIQIQDIVIRNVEVLNTKKKRDI
                     LSMIEFEITQYIPININNYSIKYKVINSKNDKLNIQVILFPKSIIQLIKNISENLNMN
                     PKTININFDILQKLINLNLIENFSEEGVFIECKKSQLIINITKDKKIRETYLLSKEGK
                     SYEFIK"
     gene            complement(727..1086)
                     /locus_tag="KR250_002144"
     CDS             complement(727..1086)
                     /locus_tag="KR250_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HBH0068078.1"
                     /translation="MINLINKKRKGFTLVEMIVVVTILGVISSIALVKYSKVQESAKL
                     NADYTNAANIVTAASMAINDDEKTIDSLSVETLKEKGYLNTVPVPQSTSGKFELVIND
                     SGTDISVNINSKQFYPK"
     gene            complement(1140..2225)
                     /locus_tag="KR250_002145"
     CDS             complement(1140..2225)
                     /locus_tag="KR250_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HBH0068079.1"
                     /translation="MRLSKNIFYNLEKFYLVLNRISDKEIRVICKEIGILLESGCEIT
                     KIFEIIESQSSKKAKNLLSIVSNHIQKGNSIAESFQITGIFSKFFISMIKAGETSGNL
                     DIIMSDLSNYYDKEYKLKMKIITISIYPIILIILSILSMLFIFVFVIPNFQVVFTNNG
                     IEPPLITRVLMGISTVVTNNLAYIIFSFILFSIGSIYFFITNDNIKKLINSLKFKIPF
                     IKKINQLVATTRFSRALSVLISSGIQIVEAIDMSASVIDNEFIYERLKISNNYIKKGN
                     SIGKSLNLANVFPKLFISMANTGEESGKLDKSLNVINKFYENELNNKIEQIMKFIEPM
                     LIVFMGLIIGTFILAMVVPMFDAITNF"
     gene            complement(2396..3775)
                     /locus_tag="KR250_002146"
     CDS             complement(2396..3775)
                     /locus_tag="KR250_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HBH0068080.1"
                     /translation="MIKIILEESSLPMLIPEKLAKKYNVFPVEFQDDNLVVEIEDEDI
                     YALQDLRLATGKEIILKKEKKELISEKIEKYYNSFELDEDYAKKLFENLLEKAIKENA
                     SDIHIEPFKEHLIVRMRVDGELKEVSKFLVDVYPSLSTVIKLKASMDITEKRLPQDGR
                     VDIKLGGNLIDIRVSSIPTVYGEKIVLRILNRDTFLKDKLELGFSEEAIKSINRIINK
                     RAGILLVTGPTGSGKTTTVYSLLNDLKGINKNIMTIENPVEYKMDGISQIQVNSKVGL
                     SFDVGLRAILRQDPDIIMVGEIRDAETAKIAVRAAITGHLVISTLHTNDAVSSIARLL
                     EMQIPPYLLNASLIGVISQKLVRKVCNHCSHEIMIKDNFSGDVNTKVAVGCEKCNDKG
                     YFGRTAIYEILEINDDIKTCIRNMEDSSTIKDVAQNNGMITFEDSCKRLINEKITTLE
                     ECLVVNEMI"
     gene            complement(3768..4448)
                     /locus_tag="KR250_002147"
     CDS             complement(3768..4448)
                     /locus_tag="KR250_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HBH0068081.1"
                     /translation="MKKQYVFIILASTILGILVGTIFRDYSNEETVYTSKDKSIRKEI
                     KMTRKSIKELTKEKDKLDKEINSMKQNQKNSEEISEIETLRDSLSYSDVKGSGIILSI
                     DAIDEKTGNIANSIDYNKILVKVINELKVNGGKYIEINGQRINQYSEVVLAGSHININ
                     GVPVAQPYEIKCIGDKEKLSNYVDKGSKYLDSIQTNYPMKISIKTEENISMKKMNIPN
                     KLKYIKGD"
     gene            complement(4840..5919)
                     /locus_tag="KR250_002148"
     CDS             complement(4840..5919)
                     /locus_tag="KR250_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBH0068082.1"
                     /translation="MEGKVKKAFPGGNTSQGSYSFFDYIIPENVKRVFCLKGGPGVGK
                     SSLMKKIYKEFLHRGYDIDLYHCPSDPSSLDGLVIKKLGVVLMDATAPHTMDPKLPGA
                     LDEIINLGDYWNTEELEKNKQEISVYDKDISNSFKRAFKFLKSAEPIFRDIEEKYSDC
                     MDYGKVNLVTEEFIKRLFDGVKSTGNLKREKHLFGSAITPVGCLDYTENILKDVKKVY
                     YLDGEIGTGKTTLLNKVYKKAIEKGLNVEVYHYPLIPEKIETVLLTDLDIGITISTTF
                     KEEDTIDLNQFLNREKLLKYEEDIKFDEKVLDDLICWAVLNLKRAKSKHDIIESYYSP
                     NMRFDEVGKLRDKLIKRILKYEENL"
     regulatory      6207..6322
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            6497..7867
                     /locus_tag="KR250_002149"
     CDS             6497..7867
                     /locus_tag="KR250_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBH0068083.1"
                     /translation="MKQKWIVLIIICIGVFMSTLDGSILNIANPTIAADFKINMSQIQ
                     WVVTAYMLVVTATMLFFGKLGDKVGSNRLYTLGFFIFTIGSFLCSMSNNLSTLISSRI
                     FQAVGASILMATGLGIVSNAFPANEKGKAIGITGAVVGIGNMSGPVIGGIILEHFGWP
                     SIFIINIPIGIIAVFLGIKFLPKPVLDEQNKSFDIPGLLLFASCTTLILLAMNEKGNT
                     RLYLGITALIIFLLLALREVKFEQSFIDLPLFKNRNFTVGNIIGVACYFPQMAVSFLL
                     PFYLEQLKNLSPMMAGYVMTVHPLIMVLIAPIAGSLSDKHGAKNILTASFSFMTISLV
                     GMALLKADSPLYLLIVCLVIFGLGLGAFSSPNNSSILADVPPQKQGYGGSFLATIRNL
                     SFALGTAFFSSFFAQSLTYNQKFKSHTSAYVIASNQSYWIAASVCFVGLILTVFFMRK
                     TDKSIS"
     gene            complement(8133..8744)
                     /locus_tag="KR250_002150"
     CDS             complement(8133..8744)
                     /locus_tag="KR250_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HBH0068084.1"
                     /translation="MKIRSSEITMSAVNKSQYPAEGIPEIALAGRSNVGKSSIINTLL
                     NRRNFARTSQTPGKTRTINFYLINNEFYFVDLPGYGYAKIAKSEKEKWGGIMERYLES
                     RQELCSIFLLVDIRHEPTADDKLMYEWIKHFGYNCVVIATKADKISRGQYQKHISIIR
                     KKLQMESSEKVIPVSSLKKTGVEELWEEIVNQYNQHGYEITVD"
     gene            complement(8734..11097)
                     /gene="lon"
                     /locus_tag="KR250_002151"
     CDS             complement(8734..11097)
                     /gene="lon"
                     /locus_tag="KR250_002151"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HBH0068085.1"
                     /translation="MEQNYTKIDHELPLIPLRGLAIFPYMILNFDIGREISLKALDQA
                     MMDEELIFLTSQKEAEVDEPGEEDFYHVGTICKVKQMIKLPGDTVRVLVEGVSRGRVK
                     KIEQEDGYFRAVIEEIVFDSDNLDSETEVEIEAFVRNVFDAFEEYINIGNRVSPEILI
                     SLADIEDVDRFIDTIAANIYLKSSQKQEILEEFDIRKRLELIYSILLEEIDILKIEKK
                     ITLRVKKQMNKVQKEYYLREQLKAIQKELGEEEDINSEADEYREKLKKIKAPKTTKEK
                     IEKEIDKFSKISSMSPDVSVSRNYLDTIFSLPWNKETKDKLDITKAKDILDEDHYGLE
                     KVKERILEYLAIRTLAKSLKGPIICLVGPPGTGKTSIVKSIARALNRKFVRISLGGVR
                     DEAEIRGHRRTYVGSIPGRIINGVKEAQTKNPVFLFDEIDKMAADYKGDPASAMLEVL
                     DPEQNKDFVDHYLEIPFDLSKILFVTTANSLSNIPRPLLDRMEVIEVSGYIEEEKLNI
                     AKKYLLPKQIKEHALKENFIKIDDETLRSIINHYTREAGVRTLERTIGKICRKVAKKY
                     VEDPTLEEVVINKSDLETYLGKDMFKYQLAEVNPQIGLVNGLAWTEVGGVTLEVEVNV
                     LKGKGEIVLTGKLGDVMKESAKTGISYIRSIVDKFDIDPEFYKTNDIHIHIPEGAVPK
                     DGPSAGITMALAVISALTKRPVPGNIAMTGEITLRGRVLAVGGVKEKLLAAHRAGITK
                     VLIPKECEADLDEIPENVKEKMEFVLVEHMDEVLEQALLKSGENNEN"
     gene            11277..11789
                     /locus_tag="KR250_002152"
     CDS             11277..11789
                     /locus_tag="KR250_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HBH0068086.1"
                     /translation="MLKLFLTFLKIGAFTFGGGYAMVPLIQDELVNKQKLLEQEEFID
                     YLSIAQSYPGVLAVNISVLLGYKLYGIPGVLICTLGSALPSFCIVLVLSYLYFKNSSS
                     KILDGFFKGVAPVVIALILYSFTSMFKKLPKSKTNILLLIIAVLSVGVFNISPIYLII
                     GGGVYSLCQK"
     gene            11777..12304
                     /locus_tag="KR250_002153"
     CDS             11777..12304
                     /locus_tag="KR250_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HBH0068087.1"
                     /translation="MSEIILLFLIFLKIGAFSFGGGYAMLPFIQQEFISKYHFITNQE
                     FLDLLAISQSTPGPVAINSATFVGFKTAGFFGSLAATIGVTIFALIGLTIISKLFSKF
                     KNNKKVENLLTVLRPITLGFILSAAFSSAKEVNWDLYGIIAFIASFYLLYKGKIGSIA
                     IVFVFGILGILLTFL"
     gene            complement(12375..13625)
                     /gene="clpX"
                     /locus_tag="KR250_002154"
     CDS             complement(12375..13625)
                     /gene="clpX"
                     /locus_tag="KR250_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HBH0068088.1"
                     /translation="MSKYEEKRQLKCSFCGKNQDQVRRLIAGPNVYICDECVELCDEI
                     IQEEIEDTIDEDTTSLPKPKEMMEILNDYVIGQEKAKKALSVAVYNHYKRIYSKKSSS
                     KDIEIQKSNILLLGPTGSGKTLLAQTLARTLNVPFAMADATSLTEAGYVGEDVENILL
                     KLIQAADFDIEKAERGIIYIDEIDKIARKSENPSITRDVSGEGVQQALLKILEGTVAN
                     VPPQGGRKHPHQEFLKIDTTNVLFILGGAFDGLEKIIQKRGGDKTLGFGAKIESKKEL
                     DLGKLYEKVLPEDLLKYGIIPEFIGRIPVLATLELLDEDALMQILQEPKNALVKQYKK
                     LLELDDVELEFEEGALRAIAKKAIERNTGARGLRSIVESVMMETMFEVPSRDNIKKVI
                     VTEKSVNEDSVNPIIVLKDQEESA"
     gene            complement(13644..14228)
                     /gene="clpP"
                     /locus_tag="KR250_002155"
     CDS             complement(13644..14228)
                     /gene="clpP"
                     /locus_tag="KR250_002155"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HBH0068089.1"
                     /translation="MALVPVVVEQTGRGERSYDIFSRLLKDRIIFLGDQVNDATAGLI
                     VAQLLFLEAEDPDKDIHLYINSPGGSITSGMAIYDTMQYIKPDVSTICIGMAASMGAF
                     LLAAGAKGKRLALPNSEIMIHQPLGGAQGQATDIEIHAKRILKIKETLNEILSERTGQ
                     PLEKIKMDTERDNFMSALEAKEYGLIDEVFTKRP"
     gene            complement(14378..15664)
                     /locus_tag="KR250_002156"
     CDS             complement(14378..15664)
                     /locus_tag="KR250_002156"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HBH0068090.1"
                     /translation="MKAELIKKEGNKVTLKITVDNNKFEAAVNKAYNKSRNKYNIPGF
                     RKGKAPRIVIETQYGKGIFYNDAIEILFPEVYPEAIKELDIDPIDNPDLDIEEISKDN
                     GLVMVLNVEVKPEFELGNYKGIEIAKVENTVSDENVDAKLQEMVEKNARLVSVEDKAL
                     EDGDTAIIDFEGFENGVAFDGGKGENYNLVIGSNTFIPGFEEQLVGKKAGEEVEVNVT
                     FPEEYHSQDLAGKPVVFNVKINDVKVKELSALDDEFAKDTSEFDSLDELKADVRAKLE
                     EEAKNRADAETRNSVVEKVAENTEIEIPEVMIQHQIDNMLNELNYQLQYQGFGLQQLL
                     EMTGRTMEELREERKEDAKKLVKSSLVLEAITKAEGIEATEEEFKAELEKMASAYNME
                     VEKIEASLRDADKEDIKGQIKIRKTIDLLVDNATIA"
     gene            complement(15858..16328)
                     /locus_tag="KR250_002157"
     CDS             complement(15858..16328)
                     /locus_tag="KR250_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBH0068091.1"
                     /translation="MKIGIVSDTHMMIKNMEKTIPYLKECDLIIHAGDNFTDSRYIHS
                     MTNVGIIAVKGNCDFDAVEEEVVFEVANKTIFLCHGDKYGVKYGTHMLEKKATEVDAD
                     IVIFGHTHTPFREIKDGVLYINPGSTSLPRGVSYKSFVIMDIEEDDIKIEEIRI"
     gene            complement(16328..17677)
                     /gene="rph"
                     /locus_tag="KR250_002158"
     CDS             complement(16328..17677)
                     /gene="rph"
                     /locus_tag="KR250_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HBH0068092.1"
                     /translation="MTRSYDRSNEQIRPVKITRDYTKYAEGSVLIEMGETKVICTASV
                     EEKVPPFLRNSGTGWINAEYSMLPRATQQRKIRDASRGKIDGRTQEIQRLIGRAIRSV
                     VDLNKLGERTIWVDCDVIQADGGTRTASITGAFVAVAEAIYKLYKAKIIKEMPITNFV
                     SAISVGIVNGIHMLDLCYEEDSKAQVDMNIIMTDKCEFVEVQGTGEEKPFSRADLNLL
                     LELGEKGNKELIRLQREALGEISDEILGMDYGNEVVIATNNAHKLEEIGEILKDFEYK
                     VYSLKDVDLAGIEIVEDGKTFEHNALIKARTIAKKTKLIAISDDSGLEVDALGKKPGV
                     YSARYAGEHATDEENRKKLLKAMQNIPMSKRNARFVSAIAVVFPDGKEFVVRGICEGM
                     IGFEEKGKNGFGYDSLFIVKGYDKTFGEIPSVIKNSISHRANALKLMKQEFIKRVVK"
     gene            complement(17837..19861)
                     /gene="ligA"
                     /locus_tag="KR250_002159"
     CDS             complement(17837..19861)
                     /gene="ligA"
                     /locus_tag="KR250_002159"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HBH0068093.1"
                     /translation="MSVKDEINKLIETINYHNERYYNQDSPEISDYEYDKLMKELISL
                     EEEHPELKRIDSPTNRVGGKPLDKFNQIVHKTPMLSLSNAFSEQDLRDFDKRVQEYVG
                     ENVEYVVEFKIDGLSVGLTYNNGEFEKGATRGDGVVGEDISQNLMTVKSIPLKINDKK
                     ELIVRGEVYISKDNFRKVNEQQEEQEQPLFANPRNLAAGSLRQLDPKLTAKRPLDIFV
                     FNMENIEEYDLESHSESLEYLEKLGFSVSQSYKVCKSIDEVIEHIEYWTDNRGNLDYE
                     IDGMVIKVNNIKQRDEMGYTAKSPRWAIAYKFPAEKKKTKIVDIIVEVGRTGTITPSA
                     ILEPVRLAGTTVSRATLHNEDYIREKDIKINDTVLVQKAGDIIPQVLEVIKEERTGDE
                     IDFKMPEKCPVCSEPTIRLEGEAAVKCINMSCPAQIRRGIIHFVSRDAMNIDGLGESI
                     ITLLLNEKIIQDVSDLYYIKKEDVVDLERMGEKSADNLINSIEKSKENDLWRLINGLG
                     IKFIGVKAAKILAYNFKDLDEVISATSEQLEELEEFGSIMANSVVEFFKEDKNMNVIN
                     KLKNVGVNTKSLDNTGEKVEKIFEGMKIVLTGTLPTLKRNDAKDMIEKRGGKATSSVS
                     KSTTFVLAGEEAGSKLTKANDLGIKVIDEERFLELLKLSSKDEVKAVLES"
     gene            complement(20047..20985)
                     /locus_tag="KR250_002160"
     CDS             complement(20047..20985)
                     /locus_tag="KR250_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HBH0068094.1"
                     /translation="MKVLFTINYGKEKFDKIRELGYDVIHYSEDSIENNEDVNTADVL
                     VTYNPFERLDISKMKNLQYIQTTSVGIDQLPKEEVLKRNIKIANNRGGYSVPIGESIV
                     LYILEIYKNSAKFFKQQQNKEWKMDFSVSELYGQRVGFLGTGTISSEAAKRLKAFGVE
                     IWGVNTDGSSKKYFDKCFATEDMNIVFEECDIVVVAMPSTKSTDGIVNKDMFKLMKDG
                     SVFINVGRGNTVNQKDLEECVEKFRGVALDVFESEPLNKDNKLWECENVIVTPHNSWV
                     SDKNKERTFNMVYNNLKHYIMENKPVDNVVDISRGY"
     gene            complement(21087..21509)
                     /locus_tag="KR250_002161"
     CDS             complement(21087..21509)
                     /locus_tag="KR250_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3783 domain-containing protein"
                     /protein_id="HBH0068095.1"
                     /translation="MSFEKINNNNLDKIHNRKCIMLVNFNKKELSLIKNVSNFAGIRD
                     HIILSDKNGENIIRDILDGNISNECENGIKSKSVIFNNTPSTQVNAFLEGLKKVRINR
                     PFTAMVTETSIDWTLNNLIENLVQERNAMSLGKTIEHK"
     gene            21697..22980
                     /locus_tag="KR250_002162"
     CDS             21697..22980
                     /locus_tag="KR250_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugated bile salt MFS transporter"
                     /protein_id="HBH0068096.1"
                     /translation="MNTLLNNKFSIKNFSNAWVIVLGCMLIQAVPFSIVASIQPQFIS
                     YVVEGEGFSLASFSLIFTIGTIASAIASPFIGVLFSKVNIKILYLLGCIISSIAFATF
                     SICTELWQFYTVSCIVQIGATIISAIGIPVLISAWFDEESKGKALGFAFAGGSIGSIF
                     LQPLAVRLLATQGYSRSYLIFGIIALIVGIPVILFILRMPKDINEKVKSKGKKEKASD
                     TISPSVWGYTLKEAKKIKYFWIMTIGFIFVGIYVSSLSVQYPTYLRNYLNPILIGTVG
                     STFALFALIGNLFGGLLFDKFGQTKPLIISFVLVLIATLSLLLVDKSSTFAFVFAILK
                     GLSVFAYMIGPAYLTGSFFGQKEFGSIYGIVQLIFAVGFALGSTLFGLLVDNFGYRFS
                     WSIIILSVIIAYTLLIITSIGMKKLNKERLDKLMQ"
     gene            complement(23058..23573)
                     /locus_tag="KR250_002163"
     CDS             complement(23058..23573)
                     /locus_tag="KR250_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBH0068097.1"
                     /translation="MNYFVIADPDKCIGCRTCMIGCVVAHSDEDIFYQNLDEINFNPK
                     LSVIKTKEVSAPIQCRHCEDAPCAKACPNGGIVRVGNTIKINEENCIGCKTCMLACPI
                     GAIDIVTLKDVDEGKLCFRERMTANKCDFCIESPEGPACVNVCPTKAFTIVKDEDIDS
                     KVKNKRKLAIL"
     gene            complement(23573..24949)
                     /locus_tag="KR250_002164"
     CDS             complement(23573..24949)
                     /locus_tag="KR250_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase small subunit"
                     /protein_id="HBH0068098.1"
                     /translation="MIINIDKDLCTGCRECSKVCPVNAIEGEEGKPQEINLDRCVMCG
                     QCVQTCKSYASVIDEGFEFLQEKKQEREIPESINEPIFAAYNVCDIDKVKKALSDPNV
                     FTMVQCAPAVRVALGEDFGYYLGYLGAGKMAAALRALNFDRVYDTNFAADLTIMEEGS
                     ELIKRVTEGGTLPMFTSCCPAWVKFMEKNYPKLTNHLSSCKSPQQMGGAIFKTYGAEI
                     NNVDASKIFSVSIMPCTCKKYECDREEMDSSGYRDVDVVLTTRELAYLIKDMGIDFKN
                     LREEKFDSPLGSYSGAGTIFGVTGGVMEAAIRTGYELITGESIPDVEVKQVRGENGFR
                     KSTIKIGDLDLRVGVVSGLKNVIPVLEDLEKGKLDVDFIEVMTCPVGCVSGGGQPKIL
                     LEEYKELAYENRTKATYVHDEHLAIRKSHENPDIKKLYDEYLVEPLGEKSHHLLHTTY
                     CIGKEVAK"
     gene            complement(24974..25525)
                     /locus_tag="KR250_002165"
     CDS             complement(24974..25525)
                     /locus_tag="KR250_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBH0068099.1"
                     /translation="MKNKIGSFVIADPNKCTGCRACEVACFTMHNQHNNVGYTVGTVD
                     IPVIPRLYLVKGDNFCMPIQCRQCEDAPCLNSCPQKAIVKENNIMSVNEEKCIGCKTC
                     LLACPFGAIDLLPQYEDGKEVEQVILDENKKIAYKCDLCKDNEKIACISACPQEALKL
                     VTPIDDKKAKNRQAALSLLLTNK"
     gene            complement(25583..25798)
                     /locus_tag="KR250_002166"
     CDS             complement(25583..25798)
                     /locus_tag="KR250_002166"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0068100.1"
                     /translation="MQIHRELSKSENEYMCTKKEEQRYSKCEICGVLIDDTDKVYNNT
                     YNLCKKCKRNISTEKIVKYINIYDESK"
     gene            complement(25767..26600)
                     /gene="fdhD"
                     /locus_tag="KR250_002167"
     CDS             complement(25767..26600)
                     /gene="fdhD"
                     /locus_tag="KR250_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HBH0068101.1"
                     /translation="MNNIYSKTKLQENSKDKVFENSYSLVDYKYLNSFNVEVSKINEH
                     EVSIDNEEIIAEYPLSFILNDKYINTFLCTPYNLEELIVGFLKTKGYIENKKSLLKFE
                     INESERFAKVTIQEKQKNQDEQIIFLNSLDYITCAPVKSNIKINYDTIYSIMDRNLNS
                     SELFKNTGGVHSVSVFNFDKKIVTCEDVARHNAMDKAIGFCVLNDITLKDKIVAVSGR
                     ISLEMILKAAMTQIPIVVSKSAPTNLSIELSKKLNITLIGFVRGRRMNIYANPQRVIK
                     K"
     gene            complement(26649..28793)
                     /locus_tag="KR250_002168"
     CDS             complement(26649..28793)
                     /locus_tag="KR250_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(28377..28379),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase H subunit alpha,
                     selenocysteine-containing"
                     /protein_id="HBH0068102.1"
                     /translation="MEKKILTVCPYCGAGCQLYLVVKDNKILRAEPANGRTNQESLCL
                     KGRYGWDFLNDPKILTPRLKKPMIRKQGVLEEVEWDEAINYTASRLNEIKEKYGPDSI
                     MGTGSARGPGNEANYIMQKFMRATIGTNNVDHCARVUHAPSVAGLAYSLGSGAMSNSI
                     PEIENADVLFIFGYNGADSHPIVANRIVKAKKNGAKLIVTDPRVTESARIADIHLPIK
                     GGTNMVLVNAFGNVLIEEGLYNKEFVQNHTQGFDEYKEIVKPYTAKYAEKITGIPEEL
                     IRKAMREYAKGKKAMILYGMGVCQFGQAVDVVKGLASIALLTGNFGRESVGIGPVRGQ
                     NNVQGACDMGALPNVYPGYQNVTDDKIREKFEKAWGVKLSPNNGYSLTQVPNLVLKEK
                     KLKAYYIFGEDPVQSDPDASEVREALDELEFVIVQDIFMNKTALHADVILPATSWGEH
                     EGVYTCADRGFQLMRKAIEPQGDVKPDWQIISEISTAMGYPMNYKNTKEIWDELRQLC
                     PSFLGATYEKIETQGCVQWPCKSESMEDKGTMYLYEGQKFSTPNGKGNLFAAEWRPPM
                     EVEDDEYPFSLCTVREVGHYSVRTMTGNCRTLSSLEDEPGRVQINSNDAEKLGIEDDE
                     LVRISSRRGSVITRATVTDRVKEGATYMTYQWWVGACNELTIANLDPISKTPEYKYCA
                     VKLEKLEDQELAEKCVREEYQSLKDKMTATNI"
     gene            complement(29044..30102)
                     /locus_tag="KR250_002169"
     CDS             complement(29044..30102)
                     /locus_tag="KR250_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HBH0068103.1"
                     /translation="MDRLKNIRKSLLQYTFLFLLIGITIYLVFKTLDIKMLSNVMVMV
                     DKKFLFIGALAIMCHIMLEGVVMKIIIESTHKVNIRFIGFKLAIMGFYYNLITPFASG
                     SQPVQIYVLKKCKMPLSKASAVVTNKSIIFQIVVTFYCTILVLMNFAMLEKQMKMIMP
                     LVFLGIAINSFVLIMIILVILNPKKIKYFMRITIKHLSKIKFLKFLDNKIESIESFID
                     DYSKAINFFAKNKKVLISTIIVTFIQLSAYFSVSFWIYKAFNLQGYSYIYILTLQAFL
                     YMAISPIPTPGNVGANELAFFTIFKSVFPQPLMGYAVFLYGGFMYYLILIGSGIFTVI
                     THYRMKNRIGRNIVLSEN"
     gene            complement(30313..30972)
                     /gene="cprR"
                     /locus_tag="KR250_002170"
     CDS             complement(30313..30972)
                     /gene="cprR"
                     /locus_tag="KR250_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CprR"
                     /protein_id="HBH0068104.1"
                     /translation="MSKILVVDDELDMLKLIENVLKRDGHEVKVISDVEKAMNMDFNY
                     FNLIILDVMMPKMDGFEVCKTIRNKVDCPILFLTAKNMESDIMYGLGIGADDYITKPF
                     GVGELRARITAHLRRESREKKNLLTISNVQFNFLGKEVSIDGKKINFTKSEYLICEFL
                     AKNKGQVFSKERIYESVYGFDGESDISTITEHIKNIRSKLKQYDIYTIETVWGIGYKW
                     V"
     gene            31283..31912
                     /locus_tag="KR250_002171"
     CDS             31283..31912
                     /locus_tag="KR250_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3786 domain-containing protein"
                     /protein_id="HBH0068105.1"
                     /translation="MDEKITKNNYNVPFDYVRKKLKELDPHTISNLSNIPFNNDLNRF
                     TLNFFDRQYIIDYPSGEVWNSNGSLCNNFELKILIVRYLINAKGIPRTNKYVTFKEIP
                     GGNVYYPNFLNRTLYRLSEVFTSQIDKYKLVMEKIGAEKVNMGDLAYRFTYMGNTSMV
                     FILWFGDDEFPPNSNILFDSNIVYYFNAEDLAVVPDTAINAILNKINQL"
     gene            31980..32813
                     /locus_tag="KR250_002172"
     CDS             31980..32813
                     /locus_tag="KR250_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HBH0068106.1"
                     /translation="MNVENQSLSDIEIILSHRYDNGADYWTTPDKRLIKGSPFSTLDS
                     VLYLLELGVNPDDTLLKECADLIFSTWREDGRFKLYPNGGIYPCHTANAANVLCHMGY
                     ASDIRMQKTFQHFLDTQYTDGGWRCNKFSFGRGPETEYSNPLPTLNILNAFRFSNYLN
                     KESKLDKAVDFLLDHWTIKKPIGPCHYGIGTLFMQVEYPFRNYNLFLYVYVLSFYNRA
                     KKDKRFLEALKILESKMIGNKIVVERVVPKLSKLSFCKKGESSEIATTHYYEILKNLE
                     K"
     gene            complement(32919..33494)
                     /locus_tag="KR250_002173"
     CDS             complement(32919..33494)
                     /locus_tag="KR250_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1062 domain-containing protein"
                     /protein_id="HBH0068107.1"
                     /translation="MKKITWEVEYISQLPVIRYCKKCGKKTEYICSGLFRVNAQHKYL
                     DIWLIYKCSNCDSTWNSTIYSRVNPKSLNPEILEQFHTNDYNLVKQYAMNMELLHRNG
                     AEVGIPEYKIVGQEININDLTQVHIISKYPCQLKVSTLLREKLGLSRKVFDEMLDCGI
                     IRSNSGVDLKKGKLNYEIMLDIGKEKQKSSN"
     gene            complement(33787..34065)
                     /locus_tag="KR250_002174"
     CDS             complement(33787..34065)
                     /locus_tag="KR250_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0068108.1"
                     /translation="MSYKKAIHILPEELLELIQEYVDGECIYIPRKSNNKKEWGSNTP
                     TREELSIRNMQIYEDYQVGYDLQYLSEKYYLSLKSIQRIVLQEKRKDI"
     gene            complement(34374..35135)
                     /locus_tag="KR250_002175"
     CDS             complement(34374..35135)
                     /locus_tag="KR250_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase"
                     /protein_id="HBH0068109.1"
                     /translation="MKNKIEISMNNFVKNYHKSNNITTKWQELLFAYASAEDDMFQKL
                     KSVVGTSHAMPKDFLENGKTIITYFIPFDESVNNSNIGGFECSEIWARAYIETNMLIS
                     NLNDFLCEELEKIGANSYAIPGSYNFDSEKLVSDWSQRHVAFIAGLGTFGLNNMLITE
                     KGCCGRIGSIITSLELEPTKRPENEYCLYKHNNSCKKCVNKCVKEALKEDGFNRNKCY
                     EMCMINEKKYTYIGGCDICGKCIVGVPCSVINPSR"
     gene            complement(35304..>35928)
                     /locus_tag="KR250_002176"
     CDS             complement(35304..>35928)
                     /locus_tag="KR250_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008819588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="spore surface glycoprotein BclB"
                     /protein_id="HBH0068110.1"
                     /translation="DGATGPTGATGATGVTGATGPTGPTGATGATGATGASAIIPFAS
                     GIPLSLTTIAGGLVGTPGFVGFGSSAPGLSIVGGVIDLTNAAGTLTNFAFSMPRDGTI
                     TSISAYFSTTAALSLVGSTITITATLYQSTAPNNSFTAVPGATVTLAPPLTGILSVGS
                     ISSGIVTGLNIAATAQTRFLLVFTATASGLSLVNTVAGYASAGIAIN"
BASE COUNT        11040 a         6372 c         3962 g        14554 t
ORIGIN      
        1 ctttttatga actcatatga ttttccctct tttgatagca agtatgtttc tcttattttt
       61 ttatcttttg ttatatttat aattaactga ctttttttac attctataaa taccccttct
      121 tctgaaaaat tctctattaa atttaagttt ataagctttt gtaatatatc aaaatttata
      181 tttatagtct ttggattcat atttagattt tctgaaatgt tcttaattaa ttgaataata
      241 ctctttggga aaagaattac ttgtatattc aatttatcat ttttagaatt tataacttta
      301 tattttattg aatagttatt tatgtttatt ggaatgtatt gtgttatttc aaattcaatc
      361 atgcttaata tgtctctttt tttcttagta tttagaactt caacatttct aatgactata
      421 tcctgaattt gaatgttaaa gtataaattt ttatataata tttttgattt accaatctcc
      481 tcttttacaa atctaactac atcttctata tatttaatct tctcaatcga attactatca
      541 ttttttatat caaagttaat ctcttttata attgatttat ctataaaaac ttttttatga
      601 ctttttctta aaaatacaag ccttatataa tcatcattta tttctaagtt tagaattttt
      661 ttaattatag ccatctcctt cttaaataaa aataatttta aaacttacag caatcaatat
      721 aaaatattat tttggataaa attgcttaga atttatattt acgcttatat ctgttccgct
      781 atcatttatg acaagttcga atttacctga tgtactctga ggaactggaa cagtatttag
      841 gtatcccttt tccttcaatg tctctacact tagagagtct attgtctttt catcatcatt
      901 aattgccatg ctagctgcgg ttactatatt agcagcattc gtatagtctg catttaattt
      961 ggcactttct tgaaccttac tatacttaac taatgctata ctagatataa cgcctaaaat
     1021 agttactact acaatcattt caacaagtgt aaatcctttt cgttttttat ttatcaaatt
     1081 tatcaattaa atattctctc cttttcaaaa gtttcatcta ataaaattta tttttctctc
     1141 taaaaattag taattgcatc aaacatagga acaaccattg ctaaaataaa agtaccaata
     1201 attaatccca taaatacaat tagcataggt tctataaatt tcattatttg ctctattttg
     1261 ttatttaatt cattttcata aaatttattt attacattta gacttttatc tagtttccca
     1321 ctttcttctc ctgtatttgc cattgaaata aataattttg gaaaaacatt tgctaaattc
     1381 aaagatttac ctatgctatt tccttttttt atataattat tggaaatctt taacctttca
     1441 taaataaatt cgttgtctat aacactagct gacatgtcta tagcttccac aatttgtatt
     1501 ccactgctaa ttaaaacgga caatgccctt gaaaatcttg ttgtagctac taactgatta
     1561 atttttttta taaaaggtat tttgaatttt aacgaattaa tcaatttttt tatattatca
     1621 tttgttataa agaaataaat gcttcctatt gaaaacaata taaaactaaa tattatataa
     1681 gctaagttat ttgtaaccac tgtagatatt cccattaaaa ctctagttat taaaggtggt
     1741 tcaattccat tgttggtaaa aaccacctga aaattaggta ttacaaatac gaatataaac
     1801 aacattgata agatagataa tattataagt attattggat atattgaaat agttattatt
     1861 ttcattttta atttgtattc tttatcataa taattagata aatcactcat tattatatct
     1921 aagtttccac tagtttctcc tgcttttatc atacttataa agaatttaga aaatatacct
     1981 gttatttgaa atgattctgc tattgagtta cctttttgaa tatgatttga tactatacta
     2041 agcaaattct ttgctttttt acttgactga ctctcaatga tttcaaatat ttttgtaatt
     2101 tcacatccag attccagcaa tatgcctatc tctttgcaaa ttactctaat ttctttatca
     2161 ctgattctat ttaatactaa ataaaacttt tctaaattat aaaatatatt tttagataat
     2221 ctcatgtact ataatacctt ccaaattata ttaattacaa ttaaataaac ttaatatttt
     2281 ctaatttaat gcatttaatt ttatttcact taaatataat ttatactcaa acatcttaat
     2341 taaaatagat taaataaaga caactgacca aacgaagttt aactatacaa ttcagttaaa
     2401 tcatttcatt tactactaaa cactcttcta atgtcgttat tttttcattt attagtcttt
     2461 tacaactatc ttcaaatgtt atcattccat tattttgtgc tacatcttta atagtactag
     2521 aatcctccat atttcttata catgtcttta tatcatcatt aatttctaag atttcataaa
     2581 ttgctgttct tccaaagtat cctttatcat tacatttttc acaccctaca gctaccttag
     2641 tatttacatc tccactaaaa ttatctttta tcataatctc atggctacaa tgattgcaga
     2701 cttttctaac caacttttga gatataacac ctataagaga agcatttaaa agatatggtg
     2761 gtatttgcat ttctagtaat ctagctatgg aagatactgc atcattagta tgtaaagtac
     2821 ttataactaa atgacctgtt atagctgctc ttactgctat cttagctgtt tctgcatctc
     2881 ttatttctcc gaccattatt atatctgggt cttgtcttaa tatcgctcta agacctacat
     2941 caaagctcaa ccctactttt gaatttactt gaatctgact tatgccatcc atcttatact
     3001 ctacaggatt ttctatagtc ataatatttt tatttattcc ttttaaatca tttaataagg
     3061 aatatactgt tgtagtcttt ccactacctg taggacctgt cactaataat attcctgctc
     3121 ttttatttat tatacgattt atagatttta tagcttcctc tgaaaaacca agttctaatt
     3181 tgtcttttaa aaaagtatct ctattaagta tcctcaaaac tattttttca ccatagacag
     3241 ttggtattga tgaaactcta atgtcaatta aatttccacc taactttata tccaccctac
     3301 catcctgagg aagccttttt tctgttatat ccattgatgc ctttaatttt ataaccgtag
     3361 acaatgatgg atagacatca actaaaaact ttgatacttc ctttagttct ccatctaccc
     3421 tcattctcac tattaaatgt tctttaaatg gttctatatg tatatcactt gcgttttctt
     3481 ttatagcttt ttctaaaaga ttttcaaata atttttttgc ataatcctca tcaagttcaa
     3541 agctattata atatttttca atcttttcac ttataagttc ttttttctcc ttcttcaata
     3601 taatttcttt acctgtagct aatctcaagt cctgtagagc ataaatatcc tcatcttcta
     3661 tttctacaac taaattatca tcttgaaact caactggaaa tacattatat ttttttgcaa
     3721 gtttttctgg tattaacatt ggcaaactgc tttcctctaa tataatctta atcacccttt
     3781 atatacttta atttattagg tatattcatt tttttcatag agatattttc ttcagttttt
     3841 atgcttattt tcattggata attggtttgt atgctatcta aatatttact acctttatct
     3901 acataattag atagtttctc cttatctcca atgcacttta tttcatatgg ttgtgctaca
     3961 ggaactccat ttatatttat atggcttcct gcaagtacaa cttctgaata ttgattaatt
     4021 ctttgaccat ttatttctat gtattttcct ccgtttactt ttaattcatt tataactttt
     4081 acaagaatct tattatagtc tatactatta gctatattcc ctgttttttc atctatagca
     4141 tctatactta atattatccc actacccttt acatctgaat atgatagact atctctaagt
     4201 gtttctattt ctgatatttc ttcagaattt ttttgatttt gtttcattga attaatttct
     4261 ttatctaact tgtctttctc tttggttaat tcttttatag attttcttgt catttttatt
     4321 tcttttctta ttgacttgtc tttgcttgta taaactgttt cttcattgct ataatctcta
     4381 aatatggttc caacaagtat acctaaaata gtacttgcta gtattataaa cacatattgt
     4441 tttttcatac tgtattgctc cttacaaatt tatttttata tgataatttt ctttaattaa
     4501 atattaaaca tttacatatg cttattatat caaagttata tattaaaatt catctttagt
     4561 atataatatc atacattgaa ctcctatcac taatttacca atatcaagta tgcattttta
     4621 gatgttctta aaattatata aactttcaat aaacattgtt ttgatattat aaaaaaacat
     4681 aaattaggaa aggaaataat aattatgaaa aacaaataat tgctttggag attattaata
     4741 tcatttttta gcaaaaaaaa gtaaaatggc atagcctcaa agctacacca atttactaaa
     4801 aattatatca ttattaacat taattttaaa catatacgtt tataagtttt cctcatattt
     4861 taaaattctt ttaattaatt tatctcttaa tttccctact tcatcaaatc tcatattagg
     4921 actatagtaa ctttctataa tatcatgttt agacttggct ctcttaagat ttagtactgc
     4981 ccaacaaatt aaatcatcta aaactttttc atcaaattta atatcttctt catatttaag
     5041 tagtttttct ctatttaaaa actgatttaa atcaattgta tcttcttctt taaaagtcgt
     5101 acttatagtt atgcctatat ctaaatcagt taatagtaca gtttcaattt tctctggaat
     5161 aagtggataa tgataaactt ctacatttaa acctttttca atagctttct tatatacttt
     5221 gtttaataat gtagttttcc cagttcctat ttctccatct aaataataga ctttctttac
     5281 atcttttaaa atgttttctg tataatctaa acaccctaca ggagttatag ctgaaccaaa
     5341 taaatgcttt tctcttttta gatttcctgt gcttttaact ccatcaaata acctttttat
     5401 aaattcttct gtgaccaaat ttactttgcc ataatccata caatcagaat atttttcttc
     5461 tatgtctcta aagattggtt ctgctgattt taaaaactta aatgctcttt taaatgaatt
     5521 actaatatct ttatcataaa ctgatatttc ttgtttattc ttttctaatt cttctgtatt
     5581 ccagtaatca cctaaattaa ttatctcatc caaagctcca ggtagctttg ggtccatagt
     5641 atgtggggca gttgcatcca taagtaccac gcctaatttc tttataacta aaccatccaa
     5701 cgaacttggg tcagaaggac aatgatataa atcaatatca tatcctctat gcaaaaactc
     5761 tttataaatt tttttcataa gtgaggattt tcctactcca ggtccaccct ttaaacaaaa
     5821 aactcttttt acattttcag gaattatata gtcaaaaaaa gaatatgagc cttgtgatgt
     5881 atttccaccc gggaatgcct tctttacctt tccttccata ttagcgcctc ctcataattt
     5941 acaatatctc ttaattaaga tattcatatt ataagaattt tattacagct ttctgatttt
     6001 gacaaattta aaacttgcca tctaatacta ataacaggtt aattttatct aattattata
     6061 gattctcata ctgtgcctta ttctatctat aaatacaatt taagtgtcca tattgaaata
     6121 tttgtattgt aatacagctg gatattactt aaatccaatt gtttccatta taattttatg
     6181 ttaaaataat attacaaaat acatctgttt ttcttcataa acgggtgaaa ttacctatcg
     6241 gcggtaaaag cccgcgagcc ttatggcata atttggtcat attccaaagc cgacagtaaa
     6301 atctggatgg tagaagaaaa tagtatatga gtacctttat gtaattttac atgagtaatc
     6361 tatacaaatc cttcaactac cgtatttatt catgaaatta gacacattca aggtacctaa
     6421 tatacaggtg ctttttttgt tgtttatttt acaattatat cgtacttata aaatctatta
     6481 agattggagt gttatcatga aacaaaaatg gatagtattg attatcatct gtattggtgt
     6541 atttatgtct actcttgatg gaagtatact aaatatcgca aatcctacaa ttgctgctga
     6601 ctttaaaatt aacatgtcac aaattcagtg ggttgtaaca gcttatatgc ttgttgtaac
     6661 agctactatg ttatttttcg gaaaacttgg cgataaagta ggttcaaatc gtctatacac
     6721 attaggattt tttatattta caataggttc ttttctgtgt agtatgtcaa acaatttatc
     6781 aacattaatt tcttcaagaa tctttcaggc agttggagca agtattttaa tggctactgg
     6841 gcttggtata gtttccaatg catttccagc aaatgaaaaa ggaaaagcta taggaatcac
     6901 tggagctgta gttggtatag gaaatatgag tggtcctgta attggaggta taattttaga
     6961 acattttgga tggccttcta tttttattat caatatccct attggtatta tagcagtctt
     7021 tcttggcatt aaatttctgc ctaaacctgt tttggatgaa caaaacaaga gctttgatat
     7081 tccaggtctt ctacttttcg catcttgcac tacgcttata ttacttgcaa tgaatgagaa
     7141 gggtaataca agattgtatt taggtataac agcacttata atcttcttat tgttggcatt
     7201 aagagaagta aaatttgaac aaagctttat tgacctccct ctatttaaaa atagaaactt
     7261 tactgtaggc aatatcattg gagtagcttg ttattttcct caaatggcag tatcttttct
     7321 acttccattt tatttagaac aattaaaaaa tttatctcct atgatggctg gttatgtaat
     7381 gactgtacat ccacttatta tggtacttat agcacctatt gcaggttcac tttctgataa
     7441 acacggtgca aaaaacattc taacagcatc tttttcattt atgaccatct cactagttgg
     7501 aatggcactt ttaaaagcag attctccatt gtacctttta attgtatgtt tagttatttt
     7561 tggacttggg ctaggagcat ttagttcacc aaacaatagt agtattttag cagatgttcc
     7621 tcctcaaaaa caggggtacg gaggcagttt tcttgcaact attcgtaatt tatcatttgc
     7681 tcttggaaca gcatttttta gtagtttttt tgcacaaagt cttacataca atcaaaagtt
     7741 taaaagtcac acatcagcct atgttatagc tagcaatcag tcttattgga ttgcagcatc
     7801 agtatgtttt gtagggctta tactaactgt tttctttatg aggaaaacag ataaatctat
     7861 ttcttaaatg ctatttatta ctgactacat aaatcaataa ttttttagtt taactatttt
     7921 atactaattt tcccatacct tactaaaatt tattagtatt tagcaaaatt actgatattt
     7981 attgtttata aacaataagg atgccaaaaa aaagttcatt aaaactattt taaggcatcc
     8041 tatattttac tttaatatta atataattaa aactaactat tattacattt attattcaaa
     8101 taaatcttat gtcaatattt aacattttaa atttaatcta ctgttatttc atatccatgt
     8161 tgattgtatt gatttacaat ctcttcccat aattcttcaa caccagtctt ttttagtgaa
     8221 gaaacaggaa ttactttttc actactttcc atttgcaatt tttttcttat tatacttatg
     8281 tgtttttgat attgtcctct agatatttta tctgccttag ttgcaataac tacacagtta
     8341 tatccaaagt gctttatcca ttcatacata agcttatcat ctgctgtagg ctcatgtcta
     8401 atatctacaa gtaggaatat agaacataac tcttgtctac tttctagata tcgttccatt
     8461 atacctcccc acttttcttt ttcagactta gctatctttg catatccata gcctggaagg
     8521 tctacaaaat aaaattcatt atttattaga taaaaattta tagttctagt ttttcctggt
     8581 gtttgactgg ttctagcaaa gtttcttctg ttaagcaatg tatttattat tgatgatttt
     8641 ccaacatttg acctaccagc caatgctatc tctgggattc cttctgctgg atactgtgat
     8701 ttattcacag cactcatagt tatctctgaa cttctaattt tcattatttt caccactctt
     8761 taataaagct tgttctaata cttcatccat atgttctaca agcacaaact ccattttttc
     8821 ttttacattt tctggtattt catctaaatc agcttcacat tctttaggaa ttaatacttt
     8881 tgttatacca gctctatgag ctgctaataa cttttcttta actcctccta ctgctaagac
     8941 tctacctctt aaagttatct ctcctgtcat tgctatattt ccaggcacag gtcttttagt
     9001 aagtgctgag attactgcaa gagccatagt tataccagct gatggtccat cttttggaac
     9061 agcaccttct ggaatgtgta tatgaatatc attcgtctta taaaattcag ggtctatatc
     9121 aaacttgtcc actattgacc ttatataaga gatacctgtc ttcgcagatt ccttcataac
     9181 atctcctaat tttccagtta atacaatttc gcctttacct ttaagtacgt ttacttctac
     9241 ttcaagtgta actccaccaa cttctgtcca cgcaagacca tttactaatc ctatttgagg
     9301 gttaacttct gcaagttgat acttaaacat gtcttttcca agatatgtct ctaggtcaga
     9361 cttatttata acaacctctt caagagttgg gtcttcaacg tattttttgg caacctttct
     9421 acaaatctta ccaatagttc tttctagtgt tcttacacca gcttctcttg tataatggtt
     9481 gattatactt cttaaagttt catcatctat ttttataaag ttttctttta aagcatgttc
     9541 tttaatttgt tttggaagta ggtatttttt tgctatattt aatttttctt cttctatata
     9601 tccagatact tctattactt ccattctatc taataatggt cttggtatat tacttagact
     9661 atttgctgtt gtaacaaata gtatttttga taaatcaaaa ggtatttcta agtaatggtc
     9721 cacaaaatcc ttattttgct ctgggtctaa tacttctagc attgctgaag ctgggtcacc
     9781 tttataatct gctgccatct tatctatctc atcaaataag aatacaggat tttttgtttg
     9841 ggcttctttt accccattaa ttattcttcc tggaattgaa cctacataag ttcttctatg
     9901 acctcttatt tctgcttcat ctctaactcc acccaaagat attcttacaa attttctatt
     9961 taatgctctg gcgattgatt ttactataga tgtctttcct gttcctggag gaccaactag
    10021 acatattata ggccctttaa gtgacttagc aagagttctt atagctagat actctagtat
    10081 tctctcctta actttttcta atccataatg gtcttcatct aatatatctt ttgccttagt
    10141 tatatctaat ttatcttttg tctctttatt ccatggcaat gagaatatag tatctaaata
    10201 gtttctactt acagatacat ctggagacat agaagatatt tttgaaaatt tatctatctc
    10261 tttttctatt ttctctttag ttgtcttagg tgcttttatt tttttaagct tttctctata
    10321 ctcatctgct tcagagttta tatcttcttc ttcacctaat tctttctgta tagcttttaa
    10381 ttgttctcta agataatatt ctttttgaac cttattcatc tgttttttta ctcttaaagt
    10441 tattttcttt tctattttta atatatctat ttcctctaat aatatagaat aaataagttc
    10501 aagtcttttt ctaatatcaa attcttctaa tatttcttgt ttttggcttg attttagata
    10561 tatgtttgca gctatcgtat ctataaatct atctacatct tctatatcag ctaaagatat
    10621 taatatttct ggtgataccc tatttccaat atttatatac tcttcaaagg catcaaatac
    10681 atttcttaca aatgcttcaa tttcaacttc tgtttcacta tccaaattat cactatcaaa
    10741 tactatttct tctattactg ctctgaaata cccatcttct tgttctattt tctttactct
    10801 accccttgat acaccttcaa ccaaaacacg aactgtatct cctggtaatt ttatcatttg
    10861 cttaacttta cagattgttc ccacatgata aaaatcctct tctcctggct catctacctc
    10921 tgcttctttc tgagaagtta aaaatataag ttcttcatcc atcatagctt ggtctaaagc
    10981 tttaagagat atctctctac ctatatcaaa atttaatatc atatatggaa atatagctaa
    11041 tcctcttaga ggaattaatg gtaattcatg atctatttta gtataatttt gttccatcaa
    11101 cattactcct tttctagtct aattgctttg cttttaatat tatatatttg aacaagttaa
    11161 ttttcaacat taatgataaa ttcttagaat actatcatac tatattatct ttttattatg
    11221 atataatatt aatattagta tacttagatg ttatttatct tttggaggtt tctattatgt
    11281 tgaaattgtt tttaaccttt ttaaaaattg gtgcgtttac atttggtggt ggatacgcta
    11341 tggttcctct tatacaagat gaacttgtaa acaaacaaaa acttttagag caggaagaat
    11401 ttattgatta tttatccata gctcaaagtt atcctggagt actggcagtt aatatatctg
    11461 tattattagg atataaactt tatggtatac caggagttct tatatgtacc ttaggctctg
    11521 ctttaccatc attttgtata gtcttagtct tatcgtatct atactttaaa aattcatcat
    11581 ctaaaatttt agatggattt tttaaaggag ttgcgcctgt tgtaatagct cttatattgt
    11641 attcttttac gagcatgttc aagaaactac caaagtctaa aactaacata ctactattaa
    11701 ttatagcagt tttatctgtt ggtgtattca atataagccc tatttattta ataattggag
    11761 gaggtgtata tagtctatgt cagaaataat actactcttc ttaatattcc ttaaaatagg
    11821 ggcatttagt tttggtggcg gttatgccat gctaccattt atccagcaag aatttatatc
    11881 taaatatcac tttataacta atcaagagtt tttggattta cttgctatat cacaatctac
    11941 accaggtcct gtagcaataa atagtgctac atttgtagga tttaagactg ctggcttctt
    12001 tggttctcta gccgcaacta ttggtgttac aatttttgca ttaataggtc taactattat
    12061 atcaaagtta ttttctaaat ttaaaaataa taaaaaggta gaaaacttat tgacagttct
    12121 tagaccaatt actctaggat tcatattatc agcagctttt tcatctgcca aagaagttaa
    12181 ttgggattta tatggaataa ttgcatttat agcatcattt tatcttttat ataaaggaaa
    12241 aattggttcc attgcaattg tattcgtctt tggtattcta ggtatattac tcacattttt
    12301 ataataatga tacctatttt atatttagca tattaacata aaaaaaggag cttttaaagc
    12361 tccttttcat catcttatgc actttcctct tgatccttaa gtactatgat aggatttact
    12421 gagtcttcgt tcacagattt ctctgttact ataaccttct ttatattatc tctagatggt
    12481 acttcaaaca tagtttccat cattacgctt tcaactatac ttctaagtcc tctagctcct
    12541 gtatttcttt ctatggcctt tttagcgatt gcacgaagag caccttcttc aaattctagc
    12601 tcaacatcat ctaactccaa taacttttta tactgtttta ctagagcatt tttaggttcc
    12661 tgtaatatct gcattaatgc atcttcatcc aacaactcta aagtagctaa aactggtatt
    12721 cttcctataa actctggtat tataccatat tttaataaat cctctggaag tactttttca
    12781 tatagcttcc ctaaatctaa ttctttttta ctttctattt tagctccaaa acctagtgtt
    12841 ttatctccac cacgtttttg aattatcttc tctaatccat caaaagctcc acctaatata
    12901 aataatacat tagtagtatc tatctttaaa aattcttgat gaggatgttt tcttcctcct
    12961 tgtggtggaa catttgcaac agtaccttct aatattttta gaagtgcttg ttgaacacct
    13021 tcaccactaa catctctagt tatagatggg ttttcagatt ttcttgcaat cttatctatc
    13081 tcatctatat atattatacc tctttcagct ttttctatat caaaatcagc agcttgaata
    13141 agttttaaaa gtatattttc aacgtcttct ccaacatatc cagcttcagt caatgatgta
    13201 gcatctgcca ttgcaaaagg tacatttaga gttcttgcta gagtttgagc aagtaatgtc
    13261 tttccagaac ctgtaggtcc taataaaagt atattactct tttgaatttc tatatcctta
    13321 ctactagatt ttttactgta tattctctta taatgattat aaaccgcaac tgataaagct
    13381 tttttagctt tttcttgacc tattacataa tcatttagaa tctccatcat ttcctttggt
    13441 tttggtaaag atgtagtatc ctcatctata gtatcttcta tctcttcttg aataatttcg
    13501 tcacaaagtt caacacactc gtcacaaata tatacatttg gacctgcaat taatcttctt
    13561 acttgatctt gatttttacc gcagaatgaa cactttaatt gtcgtttttc ttcatattta
    13621 gacatattaa cacctctctc tatctacggt ctctttgtaa atacttcatc tattaaacca
    13681 tattctttgg cttctagtgc actcatgaag ttatcacgtt cagtatccat tttaattttt
    13741 tctaatggct gaccagttct ttcagataat atctcattta aagtttcttt tatttttaaa
    13801 attctctttg catgaatttc tatatctgtt gcttgacctt gagctccacc taatggttga
    13861 tgtatcatta tttcactatt tggtagtgct agtctttttc cttttgctcc tgctgccaac
    13921 aagaatgctc ccatagaagc agccatacct atacatatag tacttacatc tggtttgatg
    13981 tattgcatag tatcatatat agccattcca gaagttatac ttcctcctgg agaatttata
    14041 tataaatgta tatctttgtc tgggtcttca gcttctaaaa ataatagctg tgctactata
    14101 agccctgctg tggcatcgtt aacttgatct cctaagaaga ttattctgtc ttttaatagt
    14161 ctagaaaaaa tatcataaga tctttctcct cttcctgttt gttcaacaac tacaggtact
    14221 aatgccatgg tttcataccc cctttattct atataattta cagtagcttt ttttaagcta
    14281 ctgtaaatta ttatacgcat ttaactaaat tatatttgca catcattata tactaaacat
    14341 ttcataacgt caactatatt agttagcgtt tattttatta agctattgtt gcattatcaa
    14401 ctaataaatc tattgttttt cttattttta tttgaccttt tatatcttct ttgtcggcat
    14461 ctcttaaaga agcttctatt ttttctactt ccatattgta agcagaagcc attttttcta
    14521 actcagcctt aaattcttct tctgttgctt caattccttc agctttagtt attgcttcta
    14581 atactaaaga agacttaact aattttttag catcttcttt tctctcttct cttaattctt
    14641 ccatagttct accagtcatc tctagtaatt gttgtaatcc aaatccttga tattgtaatt
    14701 ggtagttaag ttcatttaac atattatcta tttgatgttg tatcataact tctggtattt
    14761 ctatttctgt attttcagca actttttcta ctactgaatt tctagtttct gcatctgctc
    14821 tatttttagc ttcttcttct aacttagctc ttacatctgc ttttaattca tctaaagaat
    14881 caaattcact agtatcttta gcaaattcat catcaagtgc agataattct tttactttta
    14941 catcatttat ttttacatta aatacaactg gttttccagc taaatcttgt gagtgatatt
    15001 cttctgggaa agtaacatta acttctactt cttcaccagc ttttttacct actaattgtt
    15061 cttcaaatcc tggtataaat gtgtttgaac ctataactaa gttataattt tcacctttac
    15121 caccatcaaa agctacacca ttttcaaaac cttcgaagtc tattatagct gtatctccat
    15181 cttctaaagc tttgtcttct acacttacta atctggcatt tttttcaacc atttcttgta
    15241 gtttagcatc aacattttcg tcacttacag tattctcaac cttagctatt tctataccct
    15301 tgtagtttcc taattcaaac tctggtttaa cttctacatt aagaaccata accaacccat
    15361 tgtctttact tatttcttct atatctaaat ctggattgtc tattgggtct atgtctaatt
    15421 ccttgatagc ttctggataa acttctggga ataatatttc tatagcatca ttgtagaata
    15481 taccttttcc gtattgagtc tctataacaa ttcttggagc tttacctttt ctaaatccag
    15541 gtatattgta tttatttctg cttttattat aagccttatt tactgctgct tcaaatttat
    15601 tgttatctac tgttattttt aaagtaactt tattaccctc ttttttaatt agttctgctt
    15661 tcatccttct aatcctccta gtgatattta tgtaataccc gataacggta ttataattca
    15721 ttctatttat aattcaacca cttaattata tcacataagt ttttttattt acaagtgttc
    15781 cattaatatc gttgtactta tttgataaaa tttaattttt tgagcgtttt ttgccatttt
    15841 tttagataaa tacaccttta tatgcgtatt tcttcaattt ttatgtcatc ttcttctata
    15901 tccataatga caaaactttt atacgatact cctcttggaa gtgaagtgct tccaggattt
    15961 atgtaaagta caccatcttt aatttctcta aatggggtat gggtatggcc aaatataact
    16021 atatcagcat caacttcagt tgcctttttt tctaacatat gagttccata ttttacgcca
    16081 tacttatctc catggcataa gaatatggtt ttatttgcca cttcaaagac aacttcttcc
    16141 tcaacagcat caaagtcaca attaccttta actgcaataa tacctacatt agtcatgcta
    16201 tgaatatatc ttgaatcagt aaagttatct cctgcatgaa ttattaggtc acattctttc
    16261 aaatatggta ttgttttttc catattttta atcatcatat gtgtgtcgct tactatgcct
    16321 attttcatta cttcactacc ctttttataa actcttgttt cataagtttt agtgcgtttg
    16381 ctctatgact gattgaattt tttataacac taggtatttc accaaaagtc ttatcatatc
    16441 cttttactat aaataagcta tcatatccaa aaccattttt acctttttct tcaaatccta
    16501 tcattccttc acaaattcct cttactacaa attcttttcc atcaggaaaa actactgcta
    16561 ttgcagagac gaatcttgca tttcttttgc tcattggtat attttgcata gcttttaaca
    16621 gtttttttct attttcttca tctgttgcat gttctcctgc atatctagct gaataaacac
    16681 caggtttttt gcctaaagca tctacttcta agccagaatc atctgatatt gctataagtt
    16741 tagtcttttt tgctattgtt ctagctttta tcaacgcatt atgctcaaat gtttttccat
    16801 cttctactat ctctatacct gctaaatcaa catcttttaa agagtacact ttatattcaa
    16861 aatcttttaa gatttcacct atttcttcta gcttatgagc attattagta gcaatcacta
    16921 cttcattacc ataatccata cccaatatct catctgatat ttcacctaaa gcttctcttt
    16981 gaagccttat taattcttta ttcccttttt cacctaactc taacaataaa tttaaatctg
    17041 ctctcgaaaa aggtttctct tctcctgtcc cctgtacttc tacaaactca cacttatcag
    17101 tcattattat attcatatct acctgtgctt tagaatcttc ttcataacat aaatctagca
    17161 tgtgtattcc atttacaata ccaacactaa ttgcagacac aaaatttgtt attggcatct
    17221 cttttattat ctttgcttta tataatttat atattgcttc tgcaactgct acaaatgccc
    17281 ctgttataga tgcagttcta gttccaccat ctgcctgaat cacatcacaa tctacccaaa
    17341 tagttctttc tcctaattta ttcaagtcta caacagacct tattgctctt cctattaacc
    17401 tctgtatttc ttgtgttctt ccatcaattt ttcccctaga tgcatctctt atctttcttt
    17461 gttgtgttgc tcttggtagc atagaatatt cagcatttat ccatccagta ccactatttc
    17521 ttaaaaaagg aggaactttt tcttccactg atgcagtgca tataaccttt gtctctccca
    17581 tttctataag aacagaacct tctgcatact ttgtataatc tctagttatc tttacaggtc
    17641 ttatctgttc gttacttcta tcataacttc tggtcatttt ttatatcctt tctcatattt
    17701 tttatttttt ataaatttat ttttataatt tttaattttg tcaaatatta attattgtca
    17761 tttttcgtaa tcttattagc ttactgaact attctatcat taattatagt agttttctac
    17821 taaattaagc gactttttaa ctctctaata cagcttttac ttcatctttc gatgataact
    17881 ttaataattc taaaaatctc tcttcatcta taactttaat tcctaaatca tttgctttag
    17941 taagtttaga accagcttct tcacctgcca aaacaaatgt tgtcgattta ctaacactag
    18001 atgtagcttt tccacctctt ttttctatca tatcttttgc atcatttctt tttaatgtag
    18061 gaagtgtacc agtcaatacg attttcatcc cttcaaatat tttttctact ttttcacctg
    18121 tattatccaa tgattttgta tttactccta cattttttaa cttgtttata acgttcatat
    18181 ttttgtcttc cttaaaaaac tcgacaacgc tgtttgccat tatacttcca aattcctcta
    18241 attcttctag ttgctcacta gttgcagaaa ttacttcatc taaatcctta aagttatatg
    18301 caagtatctt agctgcttta actcctataa atttaatacc taatccattt ataagtctcc
    18361 ataaatcatt ttcttttgat ttttcaattg aatttattaa attgtcagca gatttttcgc
    18421 ccattctctc taggtcgact acatcttcct tttttatata atataaatca gatacatctt
    18481 gaataatttt ttcatttaat agcagtgtaa ttatagattc tcccaatcca tctatattca
    18541 ttgcatccct tgacacaaag tgtataattc cccttcttat ttgggctggg caagacatat
    18601 tgatacattt tacagcagct tctccttcta atcttattgt tggctctgaa caaactggac
    18661 acttttctgg cattttaaaa tctatttcat ctccagtacg ttcttctttt ataacttcta
    18721 aaacttgagg tattatgtcc cctgcttttt gaactaatac tgtatcattt atttttatat
    18781 ctttttctct tatataatct tcattgtgaa gtgtagctct acttacagta gtacctgcaa
    18841 gtctgactgg ttcaagaatt gctgaaggtg ttattgtacc agttctccca acttctacta
    18901 ttatatctac tattttagtt ttcttctttt ctgctgggaa tttatatgca attgcccatc
    18961 taggactttt ggctgtatat cccatttcgt ctctttgttt tatattgttt acttttatta
    19021 ccataccatc tatttcgtag tctaaattac ccctgttatc tgtccagtat tcaatatgct
    19081 caataacttc atctatactt ttacaaactt tataactctg acttactgaa aatcctagtt
    19141 tttctaagta ctctagtgat tcactgtgac tttctaaatc gtattcttct atattttcca
    19201 tattaaatac aaatatatct aatggtctct ttgctgttag ttttgggtct aattgtctca
    19261 atgaaccagc tgcaagattc cttggattag caaataatgg ctgttcttgt tcttcttgtt
    19321 gttcatttac ttttctaaaa ttatcttttg atatatatac ttctcctcta actattagtt
    19381 cttttttatc atttatctta agaggtatgc tttttactgt cattaagttt tgagaaatat
    19441 cttctcctac aactccatct cctctagttg ctcctttttc aaactctcca ttgttataag
    19501 ttagacctac agaaagacca tctattttaa attcaacaac atattcaaca ttttctccca
    19561 catattcttg aactctctta tcaaaatctc tcaaatcttg ctctgaaaaa gcatttgata
    19621 aactcaacat aggagtttta tgtacaattt gattgaactt atctaatggc ttacctccta
    19681 ccctatttgt tggagaatca attctcttta gttctggatg ttcttcttct aaagaaatta
    19741 actccttcat taacttatcg tattcataat ctgatatttc tgggctatct tgattataat
    19801 acctttcatt atggtagttt attgtctcta taagtttatt aatttcatct tttacgctca
    19861 ctttttgcac ctcttcatat ttttaaaatt aaattatttt gaaatttccc tataattaat
    19921 tatctcctat ttatatattt tttagtatat cactttttgg gctttaaatt tattacaaaa
    19981 aaaactctta ttagtatctt taatacgact aataagagtt tttattttta tcaaaatttt
    20041 ttatttttaa tagcctcttg atatgtctac aacattatct acaggtttat tctccattat
    20101 atagtgtttc aaattattat aaaccatatt aaatgttctc tctttgtttt tatctgacac
    20161 ccaagaatta tgaggagtta ctatgacatt ttcgcactcc cacaacttat tgtccttgtt
    20221 taaaggttca ctttcaaata cgtctaatgc tacccctcta aatttctcta cacattcttc
    20281 taaatctttc tgattaactg tatttcctct acctacattt ataaatacag aaccatcttt
    20341 cataagttta aacatatctt tgtttactat tccatctgta ctttttgtag aaggcattgc
    20401 tacaactact atatcacatt cttcaaatac aatattcata tcttcagtag caaaacactt
    20461 atcaaagtat tttttactac ttccatcagt attgacaccc catatttcta caccaaaagc
    20521 ttttaatctt tttgctgctt ctgaagatat agtacctgtt cctaaaaatc caactctttg
    20581 tccatacaat tctgatacag aaaaatccat cttccattct ttattttgtt gttgtttaaa
    20641 aaattttgca ctatttttat atatttctaa aatatacaat acaatacttt caccaatagg
    20701 tacactatat ccgcctctat tattagctat cttaatattt ctcttcaata cctcttcttt
    20761 tggaagttgg tctataccta cactagttgt ctgtatatat tgtaaatttt tcatcttaga
    20821 aatatctaat ctctcaaatg gattgtatgt aactagtaca tctgctgtat ttacatcctc
    20881 attattttca atactatctt cagagtaatg tatgacatca tagcctaatt ctcttatttt
    20941 atcaaatttt tcttttccat aatttattgt aaataaaact ttcatctcaa atctcctcca
    21001 tctacccaca tttaaaattt tatattctaa aatataatat gatactcaat gtacattagt
    21061 taaaattact ataagatatc ttaaatttac ttatgttcaa ttgtttttcc taaagacata
    21121 gcattacgtt cttgaactaa attttctatt aaattattca atgtccaatc aatagatgtc
    21181 tctgttacca tagctgtaaa aggtctattt attctcactt tttttagccc ttctaaaaat
    21241 gcatttactt gtgtgcttgg agtgttatta aatattacag acttactctt aatcccattt
    21301 tcacattcat ttgatatatt tccatctaaa atgtctctta ttatattctc tccattttta
    21361 tcacttaaga tgatatggtc tcttattcca gcaaagttac ttacgttttt aattaatgat
    21421 aattcttttt tattaaagtt tacaagcata atacacttcc tgttgtgtat tttatctagg
    21481 ttattattat ttattttttc aaatgacatt ttatccatcc tttcatttat ttatatactt
    21541 taatttaccc catacattta aaataatcaa tttttatagt taatttttat attaataccc
    21601 aaattatatt taaatagttg ggctatccaa aactaatata taaaattaga ataattagat
    21661 taatatctac aaatcataac ctaaaggaga gataccatga atacattact aaacaataaa
    21721 tttagcatta aaaacttttc aaatgcttgg gttatagtac ttggttgtat gttgatacaa
    21781 gctgttccat ttagtattgt agcaagtata cagcctcaat ttataagtta tgttgtagaa
    21841 ggagaaggct ttagtttagc ttctttttct ctaatattca ctattggtac tattgcctca
    21901 gcaattgcat caccttttat aggtgtatta ttttctaaag tcaatattaa gatactatat
    21961 ttattaggtt gtataatttc tagtattgct tttgctactt tttctatatg tactgaacta
    22021 tggcaatttt acactgtatc atgtattgtt caaattggcg ctacaattat atctgctata
    22081 ggaatacctg ttttaataag tgcttggttt gatgaagaaa gcaaaggaaa agctttgggc
    22141 tttgcttttg ctggtggttc tattggtagt atatttctac aaccacttgc agttcgctta
    22201 ttagcaactc aaggatacag tcgttcttat ctaatatttg gaattatagc attaatagtt
    22261 ggtatacctg taatattatt catactcagg atgcctaaag atattaatga aaaggtaaaa
    22321 tctaaaggta aaaaagagaa agcatctgat acaatttctc cttcagtatg gggttataca
    22381 ttaaaagaag ctaaaaaaat taaatatttt tggattatga ctattggatt tatatttgtt
    22441 ggtatatatg tttcatcatt atccgtacaa tatccaactt atcttagaaa ttatctaaat
    22501 cctattctaa taggaacagt aggctccaca tttgctttat ttgctctcat tggtaattta
    22561 tttggaggat tattgtttga taaattcggt caaacaaagc cacttattat atcttttgta
    22621 ttggttctta tagcaacttt atcattacta ttggtagata aatcttctac ttttgcattt
    22681 gtatttgcca ttttgaaagg tctttctgta tttgcttata tgattggtcc tgcttattta
    22741 actggctctt tctttggtca aaaggagttt ggtagcatat atggaatcgt acaattaatt
    22801 tttgcagtgg gatttgcatt aggttcaaca ttatttggat tgttagttga taattttgga
    22861 tacagatttt cttggtctat tataatttta tcagttataa ttgcctatac tttacttatt
    22921 ataacttcta taggaatgaa aaaattgaat aaagaaagat tagataaact aatgcaataa
    22981 ttgattttta gatatataaa tattataata aaaaagatgg agcttttaat cataaataaa
    23041 actccatctt ttttatatta caatatagct aattttctct tatttttaac tttactatct
    23101 atatcctcat ccttaactat agtaaatgcc ttagttggac aaacatttac acatgcaggc
    23161 ccttctggcg attctataca aaaatcacac ttatttgctg tcattctttc tctaaagcaa
    23221 agctttcctt catctacatc ttttaatgta actatgtcta ttgcacctat aggacatgct
    23281 aacatacaag tcttacatcc tatacaattt tcttcattta tttttattgt gttcccaacc
    23341 ctaactatac caccatttgg acatgccttt gcacaaggag catcttcaca atgtctacac
    23401 tgtattggag cacttacttc ttttgtcttt atgacagaaa gttttggatt aaaatttatt
    23461 tcatctagat tttggtaaaa aatatcttca tctgaatgtg caactacaca tcctatcata
    23521 caagttctac atcctatgca tttatcaggg tctgctataa caaaatagtt catcacttag
    23581 ccacttcctt tcctatacaa tatgtagtgt gtaatagatg atgagatttt tctcctaatg
    23641 gttctaccag atattcatca tacagttttt taatatcagg attctcatga gattttctaa
    23701 ttgctaaatg ttcatcatgc acgtatgttg cttttgttct attttcataa gccaattcct
    23761 tatattcttc tagtaaaatt tttggttgac ctccaccact tacacaccca acaggacaag
    23821 tcataacttc aataaaatct acatctaatt ttcctttttc caagtcttca agcactggta
    23881 ttacattttt taatccactt actactccaa ctcttaagtc taagtctcct attttaatag
    23941 ttgactttct aaaaccattt tctcctctta cttgttttac ttctacatca ggaatacttt
    24001 ctccagttat aagctcatat cctgttctta tagcagcttc cataacccca ccagtaacac
    24061 caaagatagt tccagctcca ctataactac ctaaaggact atcaaatttt tcttctctta
    24121 aatttttaaa atcaattccc atgtctttta ttaaataagc tagttctcta gtagttaata
    24181 ctacatctac atctctatat ccactagaat ccatttcttc tctatcacat tcatattttt
    24241 tacatgtaca tggcattata gaaacactaa atatctttga tgcatctaca ttatttatct
    24301 ctgcaccata agttttaaat attgcaccac ccatctgctg aggtgattta caagaagata
    24361 aatgattagt taatttagga taattttttt ccatgaattt tacccaagct ggacaacaag
    24421 atgtaaacat tggaagagtt ccaccttctg tcactctttt aattaattca ctaccttctt
    24481 ccattatagt taaatctgct gcaaaattag tatcgtagac tctatcaaaa tttaaagctc
    24541 taagtgctgc tgccattttc ccagcaccta agtatcctaa ataatatccg aaatcttctc
    24601 ctaaagcaac tctaactgct ggtgcacatt ggaccatagt gaatacattt gggtcactta
    24661 aagctttctt caccttatct atatcacata cattgtatgc tgcaaatata ggttcattta
    24721 tagactctgg tatctctctt tcttgttttt tctcttgtaa aaactcaaat ccttcatcta
    24781 taactgaagc ataagattta caagtttgaa cacattgacc gcacataaca catctatcta
    24841 aatttatctc ttgtggcttt ccctcttcac cttcaatagc atttactgga cagacctttg
    24901 aacattctct acatccagta cataaatcct tatctatatt tattatcata aacgtcacct
    24961 acccctgtat cctttattta tttgttaaca ataaactaag tgcagcttgt ctattcttag
    25021 cttttttgtc atctattgga gtaacaagtt ttaatgcttc ctgaggacat gcacttatac
    25081 aagctatttt ttcattgtct ttacataagt cacatttata agctatcttc ttattttcat
    25141 ctaaaattac ttgttctact tctttaccat cttcatattg tggaagtaag tctatagctc
    25201 caaaaggaca tgctagcaga caagttttac atcctataca cttttcttca tttacagaca
    25261 taatattgtt ttctttaact atagcttttt gtggacatga gtttaaacaa ggagcatctt
    25321 cacactgtct acactgtatt ggcatacaga aattgtcacc ttttactaag taaagtctag
    25381 gtataacagg tatatcaact gttccaactg tataacccac attattatgc tgattatgca
    25441 ttgtaaaaca agctacttca caagctctac aacctgtaca tttgtttggg tctgctatta
    25501 caaaagagcc tattttattt ttcatattat cccacctttc aatttgtata tttccaaccc
    25561 tctagttatt ttcatcatat gtctacttac tttcatcata tatatttata tatttcacta
    25621 ttttctcagt acttatattt cgtttacatt ttttgcacaa attgtatgtg ttattataca
    25681 ccttatcagt atcatcaatt aatactccac agatttcaca ctttgaatat ctttgctctt
    25741 cttttttagt acacatatat tcattctcac tttttgataa ctctctgtgg atttgcataa
    25801 atattcatcc gtcttcctct tacaaatcca ataagcgtta tatttaattt tttagaaagc
    25861 tctattgata aattagtcgg tgctgatttt gatactacaa taggaatttg agtcatagct
    25921 gcttttaata tcatttccaa tgatattctt ccacttacag ccacaatttt atcttttaat
    25981 gttatgtcat ttaaaacaca aaatccaata gccttatcca ttgcattatg ccttgctaca
    26041 tcttcacaag tgactatttt tttatcaaaa ttaaatacag atacgctatg taccccacca
    26101 gtatttttaa ataattctga tgaatttaaa tttctatcca ttatactgta aatcgtatca
    26161 taatttattt ttatattgct ctttacagga gcacatgtta tatagtctag tgaatttaaa
    26221 aatataattt gctcatcttg atttttttgt ttctcttgaa tagttacttt tgcaaaacgt
    26281 tcactctcat ttatctcaaa ttttaataag ctttttttgt tttctatgta tccttttgtt
    26341 ttcaagaatc ctactataag ttcctctaaa ttatatgggg tacataaaaa tgtatttata
    26401 tatttatcat ttaaaataaa actaagtgga tattctgcaa ttatttcttc attatctatt
    26461 gatacttcat gttcatttat tttgcttact tctacattaa aagaatttaa atatttgtag
    26521 tccactagac tataactatt ttcaaaaact ttatccttac tattttcttg taatttagtt
    26581 tttgagtata tattgttcat aaaatattag cctttctgcc ttttataaat cttaaatcta
    26641 ataaatcatt agatatttgt agctgtcatt ttgtctttca aactttgata ctcttctctt
    26701 acacattttt ctgctaattc ttggtcttca agtttttcaa gtttaacagc acaatattta
    26761 tactctggtg ttttagatat aggatctaaa tttgctattg taagttcatt gcaagctcca
    26821 acccaccact gataagtcat atatgttgca ccttccttaa ctctatcagt aactgttgct
    26881 cttgttatta cacttcctct tcttgaacta attctaacta attcatcatc ttcaattcct
    26941 aatttttcag catcattaga atttatttga acacgtcctg gttcatcttc taatgaacta
    27001 agtgttctac aattaccagt cattgttctt actgaatagt gtccaacttc tcttacagta
    27061 cataaactaa atgggtattc atcatcttca acttccatag ggggtctcca ttcagcagcg
    27121 aacaaattac ctttaccatt aggagtagaa aacttttgac cttcatacag atacatagtt
    27181 cctttatctt ccatactttc actcttacaa ggccattgaa cgcatccttg agtttctatc
    27241 ttttcataag tagcacctaa gaaacttgga cataattgtc taagttcgtc ccatatttcc
    27301 ttagtatttt tataattcat aggatatccc attgctgtag aaatttcgct tataatttgc
    27361 cagtctggtt ttacatctcc ttgtggctct atagcttttc tcatcaattg gaatcctcta
    27421 tctgcacatg tatatactcc ctcatgttct ccccaagaag tagctggaag tataacatca
    27481 gcgtgaagtg ctgtcttatt cataaatata tcttgaacaa tgacaaattc caattcatct
    27541 aatgcttctc taacttctga tgcatctggg tcactttgca ctgggtcttc tccaaatata
    27601 taataagctt taagcttttt ctcttttagt actaaatttg gaacttgagt taaactataa
    27661 ccattattag gagataattt taccccccaa gctttttcaa acttttctct tatcttatca
    27721 tcagttacat tttgatagcc aggatataca ttaggaagag ctcccatatc acaagctcct
    27781 tgaacattat tttgtccacg tacaggtcct attccaacgc tttctctacc aaaattacct
    27841 gtaagtagag caattgatgc aagtccctta actacatcaa cagcttgacc aaattgacag
    27901 acacccattc cataaagtat catagctttt ttaccttttg catattctct catagctttt
    27961 cttataagct cttctgggat tcctgtaatc ttttcagcat atttagctgt atatggttta
    28021 acaatttctt tatactcatc aaatccttgt gtatgatttt gaacaaattc tttattgtaa
    28081 agaccttctt ctattaatac atttccaaaa gcatttacta aaaccatgtt tgttccacct
    28141 tttataggta gatgtatatc agcaattctt gcagattcag ttactctagg gtcagtaaca
    28201 attaatttag caccattttt tttagctttt actattctat ttgccactat tggatgtgaa
    28261 tctgctccat tatatccaaa tataaataaa acatctgcat tttctatctc tggtatagaa
    28321 ttagacattg caccactacc taatgagtaa gccagaccgg ctacagatgg tgcatgtcag
    28381 acacgagcac aatggtcaac attattagtt cctattgtag ctctcataaa tttttgcatt
    28441 atatagtttg cttcattacc aggtcctctt gcagaacctg ttcccataat agaatcagga
    28501 ccatattttt ctttaatttc atttagtctt gaagctgtat agtttatagc ctcatcccat
    28561 tcaacctctt ctagtacacc ttgttttctt atcattggct tttttagcct aggtgttaga
    28621 attttagggt catttagaaa atcccatcca taacgacctt ttaaacacaa actttcttga
    28681 tttgttctac cattagccgg ttcagctctt aatatcttat tatcttttac tactagatat
    28741 aattggcatc cagctccaca gtatggacaa actgttaaaa tttttttctc catacaattc
    28801 cccccttaga tttatcccct gataagtaaa atttatctta aagataatga gtgtttttat
    28861 aatatgcaaa atcataaata ttttgcaata ctatctatta aattcaacaa ttttctcaca
    28921 attcctctta aatttcaaaa aaaatatgtc ataataatct ttatatttca accatttaat
    28981 taaaatattc tagttcttat gacatatttt ttaatatact caatatatat tctttaaact
    29041 tatttagttt tcacttaaaa caatatttct gccaattcta tttttcattc tatagtgagt
    29101 aattacagta aatattccac ttccaatcag aatcaaatag tacataaatc caccatataa
    29161 aaatactgca tatcccatca aaggttgagg gaatactgat ttgaatatag tgaaaaaggc
    29221 taattcatta gctcctacat tcccaggtgt tggtattgga gatattgcca tatataaaaa
    29281 tgcttgtaat gtcagtatat aaatatagct ataaccttgt aaattaaatg ctttatatat
    29341 ccaaaatgaa acactaaagt aagcacttaa ttgaataaaa gttactatta tagttgaaat
    29401 taataccttt ttattctttg caaaaaaatt tattgcttta ctataatcat ctataaaact
    29461 ttctatggat tcaatcttat tatctaaaaa ttttaaaaac ttaatttttg ataaatgctt
    29521 tatagtaatt ctcataaaat attttatttt tttaggattt aaaataacta atattatcat
    29581 aattaagaca aatgaattaa tggctatccc tagaaacact agaggcataa tcattttcat
    29641 ttgtttttct aacatagcaa aattcattaa aacaagtatt gtacaataaa aagtaactac
    29701 tatttggaag ataatagact tattggtaac aactgcactt gccttactaa gtggcatttt
    29761 acatttttta agtacatata tctgtactgg ttggcttcct gaagcaaaag gagttattaa
    29821 attataataa aatcccataa tggctagctt aaaaccaata aatctaatat taactttgtg
    29881 agtgctttct ataattatct tcattactac tccttcaagc attatgtgac acattattgc
    29941 caaagcacct atgaataaaa acttcttatc taccataacc ataacatttg atagcatttt
    30001 aatatccaat gttttaaaca ctaggtaaat tgttatacct attaacaaaa ataaaaatgt
    30061 atactgtagt aaactctttc taatattctt tagtctatcc attagaaata accatccttt
    30121 gctcaagatt tatactaaat actatattat tagtatacac tatgaatctt aatattttat
    30181 aacaaaatac cttacaattt tgtcatttac aaaaaatatt aatctataaa ttaaatttta
    30241 atatatagat taatatttta caaacatttt gtagatatat ataatatttt cactaaacaa
    30301 cacttatact tattacaccc atttataccc tatcccccat acagtttcta ttgtataaat
    30361 atcatattgt tttaatttgc ttcgtatatt ttttatatgc tctgtgatag tacttatatc
    30421 actttctcca tcaaaaccat ataccgactc atatattctt tcttttgaaa atacttgtcc
    30481 cttatttttt gctagaaatt cacatattag atattcactt ttagtaaaat taattttttt
    30541 accatcaatt gaaacttctt tgcctaaaaa gttaaattga acatttgaga tagttaataa
    30601 attctttttt tctctacttt ctcgccttaa atgagcagtt atcctagccc ttaattctcc
    30661 tacaccaaaa ggtttggtta tatagtcatc tgcacctata ccaagaccgt acataatatc
    30721 actttccata ttttttgctg tcaaaaacag tattggacaa tcaaccttat ttcttatagt
    30781 tttacatact tcaaaaccat ccatcttagg catcataaca tctaatatta ttagattaaa
    30841 ataattaaaa tccatgttca tagctttttc tacatcacta attaccttta cttcatgtcc
    30901 atctcttttc aacacatttt caatcaactt taacatatct aactcatcat ctactactaa
    30961 tatcttagac ataataactc ctccatttat ccgtttaata gtattctaat ttatatattt
    31021 aataatatct ttaaaattca cattaaccat aatactctca tattatagct tacaataatt
    31081 aattactatt ttacaaataa aatattttag atacaaaaac tttatatttc aaaaacttta
    31141 tattcatttt acacgtattt acttaaatat aaaaatttat ttaacaattt attattgtaa
    31201 tattttgaat atataataaa attaataata agaaaacttt ttataaatat acttttaatt
    31261 gtttaaattg gaggaaaaaa ttatggatga aaagataaca aaaaataatt ataatgttcc
    31321 atttgactat gttcgaaaaa aactcaaaga attagaccct catacaatta gtaatttaag
    31381 taatatacct ttcaataatg atttaaatag attcacactc aatttttttg atagacaata
    31441 tattatagac tatccaagtg gagaagtttg gaatagtaat ggtagccttt gcaataactt
    31501 tgagcttaaa atcttgatag ttagatattt aatcaatgct aaaggtattc ctagaactaa
    31561 taaatatgta acttttaaag aaataccagg aggcaatgtt tattatccaa attttttaaa
    31621 caggactcta tataggcttt cagaagtttt tacatctcaa atagacaaat ataagttagt
    31681 tatggagaaa attggagctg aaaaagtaaa catgggagat ttagcttata ggtttacata
    31741 tatgggtaat acatctatgg tatttatact ttggtttgga gatgatgaat ttcctccaaa
    31801 ttcaaatata ttatttgatt ctaatatagt ttattacttc aatgcagaag atttagcagt
    31861 tgttcctgat acagctataa atgcaatttt aaataaaata aatcaattat aaatataaat
    31921 aatattataa taaagttttt gatagattta atagaatata atatgaaatg gagtaaaaaa
    31981 tgaatgtaga aaatcaatct ttatcagata ttgaaataat attatcacac aggtatgata
    32041 atggtgcaga ctattggact acacctgata agcgtttaat aaaaggttct cctttctcta
    32101 ctcttgatag tgtactttat ctattagaat taggtgtgaa ccctgatgat actttactta
    32161 aagaatgtgc tgacctgata tttagcacat ggcgagaaga tggacgtttc aaactatatc
    32221 caaatggagg tatttaccct tgtcatactg caaatgctgc taacgtactc tgtcatatgg
    32281 gatatgcatc tgacattaga atgcaaaaaa cgtttcaaca ttttttagat acacagtata
    32341 ctgatggtgg gtggcgttgc aataaattta gttttggtcg aggtccagaa actgaatact
    32401 caaatccact accaactctt aatatattaa atgcattccg ttttagtaat tatctaaata
    32461 aagaatcaaa acttgacaaa gctgtagatt ttctacttga ccattggact attaaaaaac
    32521 caatcggccc ttgtcactat ggaattggca cattatttat gcaagttgaa tatcctttcc
    32581 gtaattataa cctattcctt tatgtctacg ttttatcatt ttataatcgt gcaaaaaagg
    32641 ataagagatt tttagaagca ttgaagatac ttgaatctaa aatgataggt aataaaattg
    32701 tcgttgaacg tgtagttcca aaactatcta aattatcttt ttgtaaaaaa ggcgagtcaa
    32761 gtgaaatagc cacaacacac tattacgaaa tattaaaaaa cctagaaaaa taatagttat
    32821 atagataaaa catattaaaa aatagtggct ttaaattttt taaagtcact atttttttaa
    32881 atgatgttcc tgaaaatata ttaagttagt tattctaatc aatttgaaga cttttgcttc
    32941 tccttgccta tatctaacat aatttcataa tttaatttac ctttctttaa atcaactcct
    33001 gagttacttc taataatccc acaatccaac atttcgtcaa ataccttcct agataaacct
    33061 agtttctcac gtaataatgt tgataccttt aactgacatg gatatttact aataatatga
    33121 acttgtgtaa ggtcattaat attaatttct tgtcctacta ttttatactc tggaatacca
    33181 acttctgctc catttctatg taaaagttcc atattcatag catattgctt aacaagatta
    33241 taatcatttg tatggaattg ttctagtatt tctggattta agctttttgg attaacacga
    33301 gaatatatag ttgagttcca tgttgaatca caatttgaac acttgtatat taaccatata
    33361 tccaaatatt tatgttgagc attaacccta aataaacctg aacaaatata ttcagtcttt
    33421 tttccacatt ttttacaata cctaattaca ggtaactggg agatatattc cacctcccaa
    33481 gtaatttttt tcataaatgc aatttccttt ctataagatt actaaattaa tagaaagtta
    33541 tgaaaaggtg gaatataatc aagtattttt ttcaaaacaa gacctcctaa ttttaataaa
    33601 ttttaatttc aaacatataa aaagctacta cttcttatta gatactttac aaccagcttt
    33661 gttagctact gtataagtta ttgaaattac ttaaattata tcatccactt aaaagaaact
    33721 atacctcaaa cacttttatc aaaagaaaat gagccagtat aattataaaa ctatactggc
    33781 tcatccttag atatcttttc ttttttcttg taatacaatt ctttggatac ttttcaaaga
    33841 taaataatac ttttccgata aatattgcaa atcatatcca acttgatagt cttcgtaaat
    33901 ctgcatattt ctaattgaca actcctcacg agtaggtgta ttacttcccc attctttttt
    33961 attattagat tttctaggaa tatagataca ttcaccatct acatattcct gtattaattc
    34021 aagtaattct tctggtaata tatgtattgc ttttttatag ctcattttgc tctcctatta
    34081 aatagtaatt tttcattagg aatagcaaag ggctatttat atattaaatt tcatagttgc
    34141 aatagccctt gctatgcaaa aataaagatt aaatcaaaca taaaatctca accctcctta
    34201 tatatcacag cgatacatat aagtctatca acagtataac tggttgtcaa tcaacacaaa
    34261 ttatttatcc attttttaaa cacatttatt tagtttttat attatattta ttttgataac
    34321 taaaattttt tcattataaa ctatctttaa aattattagt caacttaaat ccattatctt
    34381 gatgggttta ttactgaaca aggaactcca actatacact ttccacaaat atcacatcca
    34441 cctatataag tgtatttttt ttcatttatc atacacattt cataacactt attcctatta
    34501 aatccatctt cttttaaagc ttctttcacg catttattga cacatttttt gcaactatta
    34561 ttatgtttat acaagcagta ttcattttct ggtcttttag ttggctctaa ttctaaactt
    34621 gtgattatgc ttcctattct tccacaacac cctttttctg taataagcat attatttaaa
    34681 ccaaaagttc caagacctgc tatgaatgct acatgtcttt gtgaccagtc acttaccaat
    34741 ttttcactgt caaaattata cgacccagga atagcatatg aattagcccc tatcttctct
    34801 agttcttcac ataagaaatc atttaaattt gaaatcaaca tattagtttc tatgtacgct
    34861 cttgcccata tttcagaaca ttcaaaccct ccaatattac tattatttac actttcatca
    34921 aaaggtataa aatatgtaat tatagttttt ccattttcca gaaaatcttt tggcatagca
    34981 tgagatgttc ctacaacact ttttagcttc tgaaacatat catcttcagc agatgcataa
    35041 gcaaacaaaa gttcttgcca ctttgtagtt atattatttg atttatgata attttttaca
    35101 aagttgttca ttgatatttc aatcttattt ttcatagtat cacccctaga taattctata
    35161 tttccaattt aatgttatta ttttatatta tataatagta ttttataata tgcgcactaa
    35221 ttaatattaa aactatgttc atcaatcatt cttaaaaaag ctcctaatat caatacgata
    35281 ttagaagcct tttcttatct aatctaattt attgcaattc ctgcacttgc atatcctgct
    35341 acagtattaa ctaatgaaag acctgaagct gttgcagtaa atactagtaa gaatcgagtt
    35401 tgtgctgttg ctgctatatt taatcctgtt acaattccac tagaaattga accaactgat
    35461 aatatacctg taagtggtgg agctagtgta actgtcgctc ctggtacagc tgtaaatgag
    35521 ttatttggtg cagtagattg gtaaagtgtt gctgtaattg taattgttga accaacaagt
    35581 gaaagtgctg ctgttgtact gaagtatgct gaaatagatg ttattgttcc atctcttggc
    35641 attgaaaatg caaagttagt caatgttcct gctgcgtttg taaggtctat tactccacca
    35701 actatactta atcctggagc cgaactacca aatccaacaa atccaggtgt acctactaat
    35761 cctccagcta tagttgtaag tgatagtggt atacctgatg caaaaggtat tattgcacta
    35821 gcacctgttg ctcctgttgc tcctgttgct cctgttgggc ctgttgggcc tgttgctcct
    35881 gtaactcctg ttgcccctgt tgctcctgtt ggacctgttg ctccatct
//
LOCUS       DAEPIN010000041        35874 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.041, whole genome shotgun sequence.
ACCESSION   DAEPIN010000041 DAEPIN010000000
VERSION     DAEPIN010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 35874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35874
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.041"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            81..1163
                     /locus_tag="KR250_002177"
     CDS             81..1163
                     /locus_tag="KR250_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HBH0068111.1"
                     /translation="MRKLSKREKDFIQYGFLLFLICITTYLVLTTLDIKLIPKIIRLV
                     NKTYIFLGILTIIVYMIIESIVTHLIINAVQKTKVKFIGIKMATMGFYYNLVTPFASG
                     SQPMQIYALTKYDVSLSKSVAIVTNKTVVFQSTVTVFCGILILLNKSLLMEQVHSVKV
                     LITFGMIMNVSMLLGGLLIVFSPKQVKMIAEVLINFLSKFKTFKFLDSKRDRINHYID
                     DYNYSIKIFIKNVRILVLSILLTVLQLIAYFSIVYCVYKASNLRGVSYVHIMTLQVFL
                     YMAISPIPTPGNVGANEVTFFTMFSNIIPKELLGYSVLLYSGFVYYFILIVSGLFTFG
                     THYTLNSWKKENKVNSNLDKVIEKLN"
     gene            1312..1473
                     /locus_tag="KR250_002178"
     CDS             1312..1473
                     /locus_tag="KR250_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III"
                     /protein_id="HBH0068112.1"
                     /translation="MKQDKQNEKQSEISKIKQSNDNKLEIAQELHVSKNIGNMLGYRI
                     DKKKMNRPS"
     gene            1814..3082
                     /locus_tag="KR250_002179"
     CDS             1814..3082
                     /locus_tag="KR250_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HBH0068113.1"
                     /translation="MEASIHTIASNNLLIIFAIVSITGIICSKLSEIIKVPDVVLYLL
                     VGILIGPSFLKFIDIRGFQIENQLILTFGSAFILYLGGKEISLKVLRNVKISVFLLAT
                     LGVVISAFIMQQVIGFTFGISAMTALLAGTIIASTDPATLVPIFNQVKIKDRVKQTVI
                     SESAFNDATGAILTSAVLAVILSNKFSLGENIYELGTMVIVGVLVGLITGILLLKLVN
                     DKPYGIFKDFAPIISVISVVVAYELSTKLGGSGYMSCFIVGIITGNKKNFKIWLSQKS
                     YDADFYVAETLGTICRMAIFIILGSQVNLAQLSKYFMPSVITVLVLMFIARPLCVLVC
                     TLVDREAKWSKSEILFMMWVRETGVIPAALCGIISAMKVPGYEVISSVVFMTILITLV
                     IQGSTTKLVAKKLGLLEEEVINISEKVSTF"
     gene            3277..4065
                     /locus_tag="KR250_002180"
     CDS             3277..4065
                     /locus_tag="KR250_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HBH0068114.1"
                     /translation="MFTVMDIVQPDTVEEAYSILNKRKTNQVIGGSAFLRMGKKRIGT
                     GIELSKLNLDYIKEYEDYVEIGSMTTFRTLETSSIIKNNFGRIIEDSVKNIIGVQFRN
                     VVTVGATVFSKYGFSDLIVALLSLDTEVELYNIGRISLEEFLNRDYEKDLLIKIYIKK
                     TNKNASYKSLRNAKSDYPILNVSVSKHMEQFKLCVGARPQKATIAKQASEFLSNNEIN
                     EINIDKAVEIASEELTFGSNMRASREYRKAMSKVLLKRAIMEVI"
     gene            4065..4514
                     /locus_tag="KR250_002181"
     CDS             4065..4514
                     /locus_tag="KR250_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HBH0068115.1"
                     /translation="MLVKININGKKKSVDIEPEEYLVDTLRKVGNLSVKRGCDTGCCG
                     LCTVLIDKKPTLSCATLTLRALDKEITTIEGLENEVKEFSEVLVKEGAEQCGFCSPGF
                     ILTVLAMKDELENPTDDEIKHYLTGNLCRCTGYMGQLRAIKTYLEVK"
     gene            4518..6782
                     /locus_tag="KR250_002182"
     CDS             4518..6782
                     /locus_tag="KR250_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBH0068116.1"
                     /translation="MNIVGKGIKKIDGMSITTGKPVYTDDLSAKDALVVKILRSPHAY
                     AKIKDIDTSKAEMVDGVECVLTYKDVPKSRFTLAGQTYPEPSPYDRLILEDVVRYVGD
                     EVAIVAAIDEKTAIKAMNLIKVKYEVLTPVLDFEEAIGNKTMVHDEDRHCNFDIGMQR
                     EKNIVSHHDYTKGDVEKVLSNCDVVIEETYYTQAQAHAMMETYRAYNYLDHMGRLVVV
                     SSTQIPFHVKRHLSRALEIPASKIRVIKPRIGGGFGGKQTACVEIFSAIVTLKTGKPA
                     KIIYDRKETFNCSTSRHAMRLKVRLGATNDGIIKAIDIKALSDTGAYGEHASTTIGLV
                     GEKTLPMYNKVEATRFIADVVYTNKMPAGALRGYGASQGCFAVESTINMLANKLKMDP
                     TDLRLKNVVTEGETTFAYGKTLNSVDLIKCINKGKELIGWNEKYPCKHLDNGKIRSVG
                     MAITMQGSGIQGLDKSSAEIKLNDCGDYTLMIGSTDMGTGSDTILAQMAAEVLDTSIE
                     NITVISADTDLVPYDPGSYASSTTYVTGMAVVKAAQDLREKIISAGATKLGVEKDEVK
                     FDGIKLTSKKGSTLLFDIAVDSTIGPDTNQLIGLASHGSPVSPPPFIAGFAETEIDTE
                     TGKVDLVNYVAVVDCGTVINKNLAKIQVEGGIVQGIGMALFEEVRYTEKGRMDTNTFM
                     QYKIPARCDIGNVKVDFVETYEPTGPFGAKSVGEVVINTPLPAIQEAVFNGIGVRINT
                     LPITPEKVFMEMNK"
     gene            6922..7233
                     /locus_tag="KR250_002183"
     CDS             6922..7233
                     /locus_tag="KR250_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0068117.1"
                     /translation="MAGNLNNMRAVNNFRGDKNILECLVSFEGRSISQRKVRVFFKEK
                     QNQIEIDFAEEEISKLVENVVLNTSYQEMLYDEIEKQLEIDCIGTWMILSKLKDGSRV
                     H"
     gene            7519..8019
                     /locus_tag="KR250_002184"
     CDS             7519..8019
                     /locus_tag="KR250_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HBH0068118.1"
                     /translation="MRKNSINIALTAAITAIYAVLTLSLGFISYGPIQFRVAEIMMLL
                     AFLDKGYIVGLTLGCFLANVIGPYGVPDIIFGTLATFISASMIYITRKLGKQNKSTLV
                     IASLWPTVINSIVVGLMLNVFFGLPLILSMIQVGFGEFVVVTIVGVPFFCFLMKKYKN
                     VINIKF"
     gene            8076..9062
                     /locus_tag="KR250_002185"
     CDS             8076..9062
                     /locus_tag="KR250_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HBH0068119.1"
                     /translation="MSKVKRLTDVWIYINDKQKFTIKELSEEFNLSAKTIQRYLKELN
                     KMGLPIQAEQGRNGGYRVLNNSYIPPVIFTEKEVMIILFALKSLQIYNYKLMEIEINS
                     IMRKVSYERKSSIKVGIENIKRYIGFVANDTNDGDFKSSNVTEFFRASSESLILNIKY
                     KCGNQILEKNVCPIGIYLNKGAWFSPAYDCKSDKIILVHIDNIIDIKRTGESKKIHIN
                     LEEWLHEVYNNLYGNSSTTKKFGEIIYLNVLLTKEGVSKIKDSSWNLDMELNEDGSGI
                     ISTFIKVEDIDCYASILFFIGNNAKVIEPKFLNKLLYDKANELRYFYEENMV"
     gene            9112..9834
                     /locus_tag="KR250_002186"
     CDS             9112..9834
                     /locus_tag="KR250_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein"
                     /protein_id="HBH0068120.1"
                     /translation="MENFNKNAKIIGIGAEGINIINEVEEKIKANMDIEKININQEIE
                     KEYVRSLLDGVDILFLIYSSEDKHIRDIIKAVSYMSNERRVLSIGMDCSEKENKEDLE
                     LGREFKINNDSIFKFVDLMNIMVESISDSCMINIDITDLKEAIVGDKGIKYSFEEFED
                     TKSYSEIADILFDRMEYIGEEFISKKGIVFVEGSPEFSIMELNDLISNIQSKVEESYE
                     VIFSLYIKENLNGNIRVGLLYN"
     gene            9863..11797
                     /locus_tag="KR250_002187"
     CDS             9863..11797
                     /locus_tag="KR250_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBH0068121.1"
                     /translation="MSENIRGTEYWLMEEFFYQETDTPQIFREESIIALGFDIKINLK
                     KVFSMGKKSEKYINENCNDKDFIKKFMSIKKGDYFLYKAYQNIEEKVKCNVSIGIVEE
                     DFENGYELSHILGHTLPVKWMHNFDTVSNCKVYTSEFYKLNNNDISSYLEIIKSQDKK
                     LLRKRIEESENNKWYLKNYYYKNVDKCVDDEELFIQPINLEQLESLKNIKTDDYILLY
                     NSEKVICENDHTMKSLFGVCQVMEGFDKEKVSALEGGYFFPVRWIEYEKENIDGNAWV
                     IFENNSIVEKYVKRFTNKMPLNTILYGPPGTGKTYNTKQMVYNLVSETSLRNKYYTGY
                     LDYKNNVEFCTFHQSYGYEEFIEGLKSDGEGNFKQEDGILKDISIEACYNALKFEKKM
                     SFELEKDSLTLETIKDRKKKLVLENIEYSESFNFEESENYVLIIDEINRGNVSKIFGE
                     LITLLEDDKRLTKSNQTIVKLPYSKEKFSLPPNLYIVGTMNTSDKSIALLDIALRRRF
                     NFEEIMPDYDLLSIVDGIDIRKMLYTINKRIEFLYDRDHVIGQAYLLAVENINDIVVV
                     FRSKIIPLLQEYFYYDSEKVGLILGGIGRNEKDKYIVYKEKLIASELFKNNNASIMES
                     KVNYCIKQNISSEELKNIYE"
     gene            11790..13058
                     /locus_tag="KR250_002188"
     CDS             11790..13058
                     /locus_tag="KR250_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrC family protein"
                     /protein_id="HBH0068122.1"
                     /translation="MSKNIYVKETYEWIRVGNGENELTEIEYEKLLKYLENNNDVLKS
                     NIIDIKYKKLRFINYVGIICFENVILEILPKLSLSDNLVKDREILLQMLSICNKIPIT
                     MNEKIRLSLKNYNLLNFFVMYFIESMQTQMKKGIYFEYINKIENLNVMRGKILLSTYA
                     KEKGISPMKIRCEYDEYSENNFLNQVLKKACISILCRINDNSIQGKIKKILSYFQNVD
                     LIYIDRKKLLDYKFYKNNDRFKDCYLLARLILLNLSMDNSQNNQEAFSILFEINTLYE
                     EYIGILIKSIWDNSFRETYIQDKSKFLLKNEQTGKKNFNLRPDIVLYDLKNEYEIIID
                     TKWKAIEVDSNVFYRSSDIYQMYAYITAYENAKRCILLYPCIQKDKNYSSWKLSESFK
                     GKFIEAKTVRLDDIKNTKNDLKKIIFNYKF"
     gene            13385..14425
                     /locus_tag="KR250_002189"
     CDS             13385..14425
                     /locus_tag="KR250_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBH0068123.1"
                     /translation="MDKHSDSVMYGGMEVYNIWGTTTVDSIIGESDVMRALKNRICKI
                     GNSDSTVAITGESGTGKELIARAIHLAGNRGNKTFVAINCAAIPEPLLESELFGYTKG
                     AFSGADSKGKIGKFELANGGVVFLDEIGDMPIQLQSKLLRVLQEKKFTRIGSNELIDI
                     DVRIISATNRDLFELVKENKFREDLYYRLNVIPLEVPPLRERGDDIQILINNFIDKYS
                     RKMKKNVYHIEPDVEQMLLKYPWPGNIRELENTIELALNLLEEDGIIHKGIINRKILE
                     YFKLNNINIKLLEESDYEISMEQDILTLEEFERAYIKKVLKFYGNDTKSKKLAAKKLG
                     ISLATLYRKIDV"
     gene            14781..16103
                     /locus_tag="KR250_002190"
     CDS             14781..16103
                     /locus_tag="KR250_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HBH0068124.1"
                     /translation="MFDIDGRPPILKCLPLSMQHILAMIVGTVTVPIVVGGAVGVSPT
                     QITLLVQYALIIAGISTLIQLYPIGNIGSRLPIIFGVGFTYVPTLVAVGGNYGLASIL
                     GAQLIGGVATVLIGIFIKKIRKFFPNIVAGTVVFTIGLSLYPIAINYMAGGVNSPTYA
                     SMQNWAVAAITLVVVIGLSQFAKGYAKLAAIFLGIIVGYCVSLVLGIINFDPIREATW
                     FAIPKPLEFGMEFDMPVIISVVIVSIINAVQAIGDLSATTMGGMDRNITDKELSSGVI
                     GSGICTMIGALFGGLPPSSYSQNVGMVAMNKVISRFVLGIAGIFMLLSGFVPKFGALM
                     TTIPYSVLGGATISIFSIITMTGIKLIITEELSSRNITIVGLAIALGMGITSVPAAQE
                     AFPSFVKLVFGESPIVIATLVAFVLNLIIPNKSLEDEAKERAVMEETN"
     gene            16400..17761
                     /locus_tag="KR250_002191"
     CDS             16400..17761
                     /locus_tag="KR250_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine/pyrimidine permease"
                     /protein_id="HBH0068125.1"
                     /translation="MKTRNTSKYDVDGIPPLKESIPLALQHLLAMIVGNMVPAILIAN
                     VVGLNQGQATMLIQGSMLAAGLATFLQLYPIPLFKGFKLGSRLPVMMGMSYVFLGACL
                     SVAAKDGLAALFGAQIAAGVIVFFVGFGVKKIRHIFTPIVSGTIIACMGLGLFATAIK
                     NLAGGEGTQTFGSPINFTVGVIVAFVIIMINKYGKGLVKNSSILIGILVGYAISLVLG
                     LVDFSAVQGAAIVSLPTPAAFGLEFRPELIVMFTIIYIIGIADMMGACTIATVGAMDR
                     EVTDEELASVVLGNSITSIISSLFAALPTGVFSQNTAIVSMNKVTSRFVIALGALVLL
                     LAGISPALGAIMTTIPSCVVGGATLVVFSSIAMSGFSIMSMDGFTEENNLIAGVSIAT
                     SMGLTTAPQVLDQFPETIGTVLGGSSIVSGAIIAILLQTLFKLKSRKSAENVTSNLEE
                     NIG"
     gene            17834..19228
                     /locus_tag="KR250_002192"
     CDS             17834..19228
                     /locus_tag="KR250_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBH0068126.1"
                     /translation="MIDILIKNAIIVTVNKDRDVIFDGAIAIQGDRILDIGNTVDIEP
                     KYLDAKKVIDANGKVIFPGFINTHNHLFQVLLKGLGDDMALHEWLNTMMFPSAKFLTE
                     QDTYDAAMLGCMEGLKSGITTMVDYMHTHNRPGLTDGIVKAYKDLGIRGVVGRGCIDL
                     GIHKELIEDVETVEKDLRRVFEKYHNSENGRIKICVAPSSMWAISEEMGKMLWNIVKE
                     YDSYFTVHISETEFARTATKDIHGEIDIKLLEKWGIVGPEVVAVHCVCITDEDMEMLK
                     KYDIKVSHNVASNMYLASGVAPVPEMLKKGINVSLGLDGAASNNAQDMVELMKLTALQ
                     HKVNNCDPLAISAEKVLEMATIEGARTLRMEDEIGSLEIGKKADLVIFNSMLSPKSIP
                     LHNPVSTLVYSASMHNVESVLVDGNVILENGKVTTIESEEQVLLNAQHAAEELCKRAN
                     VTNRMEGHKWNSLY"
     gene            19290..19760
                     /locus_tag="KR250_002193"
     CDS             19290..19760
                     /locus_tag="KR250_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HBH0068127.1"
                     /translation="MEKIKLNINDYMYEIEVEKNETLLHVLREKLNLTGAKQGCNSGS
                     CGACKVLIDGVDMKSCKLLAKNCEGKVIYTIESFANGENLHPIQEAFIEVGAVQCGYC
                     TPGMIITTQALLNRNASPNEDEIREAFKENLCRCTGYVKIIEAVKLSAARLGGR"
     gene            19763..21979
                     /locus_tag="KR250_002194"
     CDS             19763..21979
                     /locus_tag="KR250_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBH0068128.1"
                     /translation="MEKVIGKSYPLKDAALKVTGQMKYVADMKPQNVLYAKMLLSPVA
                     HAKIKSIDTSEAEKVVGVRAIATYLNTSQRKYNGSLRFYEHKIPENEVIFSDTVRFVG
                     DRVAAVAAETPEIAEKAVKLIKVEYEELPAVFTIQDAIKEDAICIHGDSNIVSQNNIN
                     GGDVEKGFKECDYIFEDEYSTPAIHHSAIETHSVIAHYDYNDKLTVHTPCQNTFGFRI
                     ILSQIFGMSLNKVRVIRPAIGGSFGGKLELTIEPVAAALSKMTRRPVKMVLTRKESMI
                     STRTRHGSFVKLKTGVNKDGEVIAQDIKIYTNTGAYASSALNVIGALSHKVFKVYKIP
                     NIKFTGMPVYTNTPIAGAMRGYGSPQIFMAQQAQFAKIAKEIGMDLVDFQNKNAVEPD
                     DVDIIFHGSLGNPRVLDCIEQGKKMFKWDEKKKLPKEEGKYLRGIGMAIGAHGNGVFG
                     AHRDVVTLTLKLNEDGTLTLLTGAHDMGNGVVTMQTMMVAEVLGITPDKVDTFETDTD
                     ACTFNLGDYASRGVFVEGGGAKKTAEKLKNLILEEAEKLLETSKEDLYLDNGCVISKL
                     DENVKASLSDVAVYSQSKSLRELTVVEDYSSPAGRTSYGVHFAEVLVDKETKDIKVVD
                     FVAVHDVGRVINPLNLEGQLEGGIHMGLGYALSEELAFDEQGRPKATDFKTYKLFRTV
                     DMPKCQVAFVDGYEKAGPFGGKSIGECAVVPVAPAVANAVYNATDVEIHSLPIKLK"
     gene            22062..22733
                     /locus_tag="KR250_002195"
     CDS             22062..22733
                     /locus_tag="KR250_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBH0068129.1"
                     /translation="MAKILGICGSPRKGATEYAVLEALKEAETIPGIETEYWSVRGKK
                     ISPCVHCDACIRKETMCIIKDDIQELEQKILEADGIIVGSPVYDMNITAQLTAVFNRL
                     RPMYLVHPGKLQNKVGAAITTGGTRYGGQELTKLPIINFFLMHEMLVSGGLGGCYIGG
                     TIWSKDGKAKGAQEDETGMDTVKRLGKGAAEAVMVSKFGLEKWNVVKEELDVKKDEKS
                     PLRDH"
     gene            23096..24310
                     /gene="dpaL"
                     /locus_tag="KR250_002196"
     CDS             23096..24310
                     /gene="dpaL"
                     /locus_tag="KR250_002196"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HBH0068130.1"
                     /translation="MLKEIKWKVNNLPKGDKENCIKFLNEEEITKVRNFHKSFPQYKE
                     TPLANLEGLAKKLGVAGVYVKDESYRFGLNAFKVLGGSYSMGRYLAQRLDTDISELGY
                     DKLTSKEIKEKLGEITFFTATDGNHGRGVAWTANKLGQKSVVLMPKGSSEFRLNKIKG
                     EGADASITDLNYDDAVRLANDYAEADDHGVMVQDTAWDGYEEIPAWIMQGYGTMAQEA
                     IEQLKEYGVDRPTHVFVQAGVGSLAGAVQGYVASIYDECPITVVVEADEADCYYKSAE
                     AGDGKPRFVGGDMPTIMAGLACGEPNTIGFEVLKNHAAAFVSAPDWVSAKGMRTLGNP
                     LNGDEKVISGESGAVTTGLLVAAMEREDLADLRKDLKLDENSRILLISTEGDTDPDKY
                     RSIVWDGEYPSI"
     gene            24437..25624
                     /locus_tag="KR250_002197"
     CDS             24437..25624
                     /locus_tag="KR250_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HBH0068131.1"
                     /translation="MLNEQRKQEVTEICQKLIQNPSSSGNEEGVVKAIEESFEKLGFD
                     SWSRDRYGNIVGCIKGNKPGKKILFDGHIDTVPVPDASKWSVPPFEGKIVDGKIYGRG
                     TSDMKGQYTAMMSAVAYFAKDTNRDFAGELYVAGVVHEEIFEGVSAREISKAVQPDYV
                     VIGESSELNLKIGQRGRGEIVVETFGKPAHSANPEKGVNAVYKMANVIQKIQQLVPPT
                     HPVLGDGILVLTDIKSSPYPGASVVPDYCKATFDRRLLVGETREGVLAPIQALLDEMM
                     KEDPELNAKVSYAVEKADCYTGNTIESERFFPGWLYDEEDEFVQAAYKGLKEAGIDSE
                     ITQYSFCTNGSHYAGEAGIKTIGFGPSKENLAHTIDEYIEQEQLFIGTEGYYGILKSV
                     YGK"
     gene            25757..27127
                     /gene="hydA"
                     /locus_tag="KR250_002198"
     CDS             25757..27127
                     /gene="hydA"
                     /locus_tag="KR250_002198"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HBH0068132.1"
                     /translation="MGTILRGGTIITSDKTYISDLRIEDEEIVEIGNNLEINGDKVID
                     ATGKIVIPGGIDTHTHFDMNAGSITTADNFETGTRAAIAGGTTTILDFAEANEGENLL
                     QGVEAYHKKASGNCYCDYGFHMTITCLNKDTFNHMEKLISDGIVSFKMYMAYDGMKVD
                     DGTIYRVLKKARDLGCIVGFHCENGDLLDVLIEENIQNGNLEPKYHPLTRPNIVEKES
                     VSRLADITKLSNSKSYVVHLSCRESLETVKMAREKNIDMIVETCPQYLLLEDNLYNKD
                     KFEGAKYVMSPPLRKKEDINYLWEGLSSGDIQTVGTDHCSFNFKGQKDLGIDDFSKIP
                     NGAPGVEHRLALLYTYGVLENRISANKFVEVTSTNAAKIFGMYPKKGEIAVGSDADIV
                     ILNINKEETISYKTQKQNVDYTPYEGFKVKCKVEDVFLRGNHVVQSCNVKEHPTGQYI
                     KRKITK"
     gene            27145..28551
                     /locus_tag="KR250_002199"
     CDS             27145..28551
                     /locus_tag="KR250_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBH0068133.1"
                     /translation="MMDILIKNAIIVTVNKEREVIFDGALVVKDNKIADIGNSKEIES
                     KYTDVKKIIDAKGKVLFPGFINTHNHLFQTLLKGLGDDMVLKDWLETMTFPAANYLEP
                     KDTYDAAMLGCIEGLRSGITTMVDYMYPHSKPGLCDGIIDAYKELGIRGILGRGCMNT
                     GAQFGVHPGIMQDVETVEKDVRRLFEKHHNTENGRIKIGVAPAAIWSNSQEMLEMLWR
                     VVKEYDDALFTVHISETPFDREAAKELHGQYDIDVLEKLGILGPNVLMVHCVYLTEKD
                     MELTKKYDMKVSHNTASNMYLSSGVAPVPEMLKKGITVSLGVDGAASNNSQDMLELMK
                     LTALQHKVNKCDPLAMSAEKVLELATIDGARAIGMEDEIGSLEIGKKADLLIFNPMLS
                     PKAIPMHNPVSTLVYSSSMKNIESVIVDGNIIMEDSKILTANEEKALKDAQDTAERLC
                     VRGTIKNRMEGHKWNSLY"
     gene            28623..29069
                     /locus_tag="KR250_002200"
     CDS             28623..29069
                     /locus_tag="KR250_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HBH0068134.1"
                     /translation="MIIKCTINNKEKELSIRPDDFLADTLRENGYVSVKKGCDTGSCG
                     LCTVMVNDKAVLSCNYLTARVEGQHITTLEGVLDEAKKFGDFLADEGADQCGFCAPGF
                     IMMVLAMKRELQNPTEEEVIDYVNGNLCRCTGYKGQLRAIHKYLNT"
     gene            29108..29902
                     /locus_tag="KR250_002201"
     CDS             29108..29902
                     /locus_tag="KR250_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HBH0068135.1"
                     /translation="MITIKEYVVPKSLDEAYELLISRKNNIILGGCGFLKLGSKNIGS
                     AIDLKDLALDYINETDDSILIGADTSLRTLELNKVIKNYCNGVISNAVSNIVGVQFRS
                     GARVGASVFAKYGFSDLIPSLLVVDAKVKLYKKGVMNLADFLENELEKDILIEVILPK
                     KDAIGVFDSIRKCTGDFAVLNGAMLKEGSIYKIAIGARPRRAKIAYKASEILSLENDI
                     EKAGEVSSEELSFGSNIRGSKEYRKDMAKALVVRMYNSIGGECDGK"
     gene            29892..32183
                     /locus_tag="KR250_002202"
     CDS             29892..32183
                     /locus_tag="KR250_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBH0068136.1"
                     /translation="MVNKEVRKIDSEAIVTGKPIYTEDLIFHKDVLTIKLLRSPHAFA
                     KIKNIDIKNALKVKGVVNIFTYKDVPNYRYSMVGESYPEASPHDQLILEDVVRYVGDE
                     VAIIAAEDERAAIKAMKLIKVEYEVMTPILDARESEGNKILIHEEDDLFCPFDFGLDA
                     TKNIVSREKMGKGDVEKEFKESDVIVEHSYMTQSQAHCMMETHRAYTYIDTNGRLVVT
                     SANQSAYHMRRQVSKALGIPLSKVRVIKPRIGGGFGGKNIAVTEIYASFVTWMTKRPA
                     KLIYTREETFAMTNTRHQMFFDIKIGSDKEGNIKAIDMKALNNTGAYGDNGPSVCSES
                     GHNVLPTYNNVPAIKFDGRTVYTNLVPGGALRGYGATQGTFALDCAIDELAHKLNMDP
                     TEIKLKNIIKKQTEGGIMNFPIRSCALDECIIRGKKLIGWDEKYPVRDIGNNKVRAVG
                     MSVGTHGSGIANIDMAIVNMRMDEDGTYKLFSGSSDLGTGSDTILAQIAAKALNTSID
                     RISVYTGDTDMCPYDTGAYASCTTYVTGNATIKSAESLKQKILKVAQKKLDLPIDDLI
                     LYEDRVCYSEDVEKFVLLSELGKESVGGGNQEVLYSSESYGIQESAKPFLAGFAEIEL
                     DKTTGEFKVINYACSTDCGTVMNPALARIQVEGGVTQGIGLAMYEDPRHGSDGKLQTS
                     NLLQYKVPTKKDIGNIIVDFVDNYEPTGPYGAKSLGEIVIHTPAPAIANAIYNATGVR
                     IRELPITSEKVYMAMKKLNQGGK"
     gene            32184..33497
                     /locus_tag="KR250_002203"
     CDS             32184..33497
                     /locus_tag="KR250_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBH0068137.1"
                     /translation="MIAIKSKYLISSADEIYTNSAVVVENDIIKDILPNEEVEIKYKN
                     IEEIIDKSDAIIMPGFVNGHMHQYGVLSRGIPANVHFTDFEGFLNDYWWPFIENRIGL
                     KEVKATTKASAIELIESGVVAFCDTLEAPNTEEGTLIEQGKILEEIGMKAILSLESCE
                     RISFENGLRCLNENSNLIKWSRENSKLINGIMCTHTSFTCSDRFIKKAKEDTKKLNAP
                     MQFHLCESIYEPNYAEKHFGKKAVDYYNDLDILDETVLASQCVKVNDEEIDILKEKGV
                     KVVHMPLSNCEVGGGFSPVPKMIKKGIKVALGTDGYINDFFEVMRGAFLMHKSVEEDA
                     SVMPANLVFRMATEHGAYVLGLQNSGKIAVGNKADIIVMEDEFKTPVTLDNIFDQIVV
                     QGKKEFISNVYIDGRHILKEKQLVDLDKKAIVKEMKEVACEFWKF"
     gene            33533..34618
                     /locus_tag="KR250_002204"
     CDS             33533..34618
                     /locus_tag="KR250_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HBH0068138.1"
                     /translation="MYNFLGKELKSPLIIGSGPLTYSAAGCKILSDAGAGAVVTKTIR
                     KERAINPAPHMVRNTANALLNNEKWTDFEPEQWIDFEIPQMKRDGTVCIASVGHTIEE
                     SSELVEKVANAGADFIELVSYDYRDLIPMLKDAKERVNIPVIVKLPPMIDEIGDFAKK
                     LEEAGADAITACDSVGPAFRIDIETGQPLLGGNGIGYLSGETIKPITLQRIYEIKKQV
                     NIPIIGLGGCVSGDDALEMIMAGADFVGICSVVILKGAQVISKIHDDLKSNLNRLGYN
                     TIENACGIVHNHMGDVNERLSFEFTYDEEKCTKCKMCERVCAYQARKLNDKMELNEEE
                     CRYCGLCISVCKPKALGRKISCSELNV"
     gene            34848..35855
                     /locus_tag="KR250_002205"
     CDS             34848..35855
                     /locus_tag="KR250_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0068139.1"
                     /translation="MVMQVQEKILELLYTINEDVVSIKNDMSEVKQEINVLKEDMSEV
                     KQEMTVMKEDMSEVKQEINVLKGDMSEVKQEMTVMKEDMSEVKQEITVMKEDMSEVKQ
                     EINVLKVDMSEVKQEMTVMKEDMSEVRQEINIMKEDMSEVRQEINTMKEDMSEVRQEM
                     TVMKEDMSEVRQEMTVMKEDTSEVKQEINVMKKDMSEVRQEITVMKEDVSEVKSEVNF
                     MQNKINNINEDMNGIKDEVSIANEKLYGIEIKIDSLECEDKSMKEIQIEQNNILGSLL
                     HNSEINKATHDNIEHNIAYIKGDTNSIKEDIAEIRRDLNLVELATSKNWSDIVKLKSV
                     K"
BASE COUNT        14140 a         3703 c         6917 g        11114 t
ORIGIN      
        1 cttatctttt ttagtcttgc aaataatagg ttaagtagta tactatgtat agtgatatga
       61 aaaaatttag gagaaaataa atgagaaaat tatccaaaag agaaaaagat tttattcaat
      121 atggattttt attatttttg atatgtatta ctacttattt agtattaact actttagata
      181 taaaactaat acctaaaatt ataaggctag taaataagac ttatatattt cttggcatat
      241 tgacaattat agtatatatg ataattgaat ctattgtaac tcatttgata ataaatgcag
      301 ttcaaaaaac aaaagtaaaa tttattggta ttaaaatggc tacaatggga ttttactata
      361 acttagttac tccatttgca tctgggagtc aaccaatgca aatatatgca cttacaaaat
      421 atgatgtaag ccttagtaaa tcagtggcta tagttactaa taaaactgta gtatttcaaa
      481 gtacagttac tgtattttgt ggaattttaa ttttgctaaa taagtcatta ctgatggagc
      541 aagtacattc tgtgaaagtc ttgataactt ttggaatgat tatgaatgta agtatgttgt
      601 taggtggact actaatagta tttagtccaa aacaagtaaa aatgatagca gaagttttga
      661 ttaatttttt atctaaattt aaaacattta aatttttaga ttcaaaacgt gatagaataa
      721 atcattatat tgatgattat aattattcta taaaaatatt tattaaaaat gtaagaatct
      781 tagttttaag tatattactt actgtgttac aattaatcgc atatttcagt atagtttatt
      841 gtgtgtataa agcctcaaat ttaagaggag tttcatatgt acatataatg acattacagg
      901 tctttttata tatggctata tcaccaattc caacaccagg aaatgttgga gctaatgagg
      961 ttacattttt tactatgttc tccaatataa ttccaaaaga attattgggg tactctgtac
     1021 tcttatatag tgggtttgta tactatttta tattaatagt ttcaggttta tttacatttg
     1081 gaacgcatta tacattaaat tcttggaaaa aggaaaataa agtaaattct aatttagata
     1141 aggttattga aaaattaaat taataaaata attagggaaa ttctatatat aaatatggtg
     1201 ttgaataagc tatattatat tgagatattt cccttttttg attaaaatta tagaaatttg
     1261 aaataataag taataaacag aaaaagtgat attgttttga aaggagaaac tatgaaacaa
     1321 gataaacaaa atgaaaaaca aagtgagatt tctaagataa aacaaagcaa tgataataaa
     1381 cttgaaatag ctcaagaatt acatgtaagt aaaaatatag ggaatatgct tggatataga
     1441 atagataaga aaaaaatgaa tagaccaagt taatgaaaat gttgaaaatg gtacatttta
     1501 aaaaatatat ataatgaaat gttttattgt taaatttaaa cttattttaa tgggggaaaa
     1561 agtcgaaaaa tatctaaaaa gatatggata taaaaaatat taatatatat atactataaa
     1621 cttgacaaaa tcaacctttt aataactagc aaaaaatagc aaaattatat aagcgttatt
     1681 taattgtcaa aaatgaaagc taaattgcat gtcctaaaaa aaatgattga taacatttat
     1741 ttgtgtgcta taattatata gtaaaacaat aagtatattg cataatgtat gcaaatcggg
     1801 aggagagacg gacatggaag catctataca cacaattgcc agcaataatt tattgataat
     1861 ttttgctatt gtaagtataa ctggaataat ttgtagtaag cttagtgaaa taataaaggt
     1921 tccagatgta gtgttatatt tacttgtagg tattttaata ggaccatcat ttttaaaatt
     1981 tatagatatt cgtggatttc aaatagaaaa tcaactaata cttacatttg gttctgcatt
     2041 tattttatat ttaggtggaa aagaaataag tttaaaagta ttgagaaatg tgaagatatc
     2101 tgtattctta ttagctactt taggagttgt gatatctgct tttataatgc aacaagtaat
     2161 aggatttact tttgggataa gtgcaatgac tgctttacta gcaggaacta taatagcatc
     2221 aacagaccca gccactcttg tacctatatt taatcaggta aagataaagg atagggtaaa
     2281 acaaactgta ataagtgaat cagcttttaa tgatgcaaca ggagctatac taacatcagc
     2341 agtgttggct gtaattttgt caaataaatt ttcattaggt gaaaatattt atgaactggg
     2401 gacaatggtt attgtcggag ttttagttgg tttaattaca ggaatactgc ttttgaaatt
     2461 agttaatgat aaaccttatg gaatatttaa agattttgca ccaataatat ctgttatttc
     2521 agttgtagtt gcatatgaat tatctacaaa attaggtgga agtggatata tgtcttgctt
     2581 tattgtaggt ataataactg gtaacaagaa aaactttaaa atttggttaa gtcaaaaatc
     2641 ttatgatgca gatttttatg ttgctgaaac tttaggtact atttgtcgta tggcaatatt
     2701 tataatattg ggtagccaag ttaatttggc tcagttaagt aaatatttta tgccatctgt
     2761 aattacagta ttagtattaa tgtttatagc aagaccactt tgcgttttag tttgtacact
     2821 tgtagacagg gaagcaaaat ggagtaaaag tgaaatatta tttatgatgt gggtaaggga
     2881 aacaggcgta ataccagcag cactttgtgg tattatttca gcaatgaaag ttcctggata
     2941 cgaagtgatt tcatctgtag tatttatgac tatactaatt acattagtaa tacaaggaag
     3001 tactacaaaa ttagtagcta aaaaacttgg attattagaa gaagaagtga taaatataag
     3061 tgaaaaagta tcaacttttt aaagttttgc tttaaaatag tacctgttaa tggctatttt
     3121 aatattgtct aatgatgatt attgaaatag tcattttttt atgtataagt aatttacaga
     3181 atttacatat tagacatatt tattatcaaa atttgttgaa gctatgatat aattagttgt
     3241 atttaaaata agaatagcaa ataagggagg aaatttatgt ttacggttat ggatattgta
     3301 cagccagata cagtagaaga agcgtactca attttaaata aaagaaaaac taaccaagta
     3361 attggtggaa gtgctttttt aagaatgggg aaaaaaagaa taggaacagg aatagaatta
     3421 tctaaattaa atttagatta tataaaagag tatgaagatt acgttgagat tggttctatg
     3481 actactttta gaacattaga aacaagttcc ataataaaaa ataattttgg aagaataata
     3541 gaagattcag ttaaaaatat aataggagtt cagtttagaa atgtagtaac agttggtgca
     3601 acagtatttt ctaaatatgg tttttctgac ttgatagtag cattactttc attagataca
     3661 gaagtagaat tatataatat tgggagaatt agtcttgaag agtttctaaa tagagattat
     3721 gagaaagatt tacttattaa gatatacata aagaagacaa ataagaatgc ttcttataaa
     3781 tcacttagaa atgcaaagag tgattatcca attctaaatg tatctgtatc aaaacatatg
     3841 gaacaattta agttatgtgt aggagcaaga ccgcagaaag ctacgattgc aaaacaagct
     3901 agtgaatttc tatctaacaa tgaaatcaat gaaattaata tagataaagc agtagaaata
     3961 gcatcagaag aactaacttt tggttctaac atgagagcat ctagggagta tagaaaagct
     4021 atgagtaaag tacttttaaa aagagctata atggaggtta tttaatgtta gtaaagatta
     4081 atataaatgg gaagaaaaaa agtgttgata tagaaccaga agaatatctg gttgatactt
     4141 taagaaaagt tggtaattta agtgtgaaaa gaggttgtga cacaggctgt tgtggtttat
     4201 gtacagtttt aattgacaag aaacctacat tatcctgtgc aacactgaca ttgagagctt
     4261 tagataaaga aataactaca attgaaggtt tagaaaatga agttaaagaa tttagtgaag
     4321 ttttggtaaa agaaggagct gagcaatgtg gtttctgttc tcctggcttt atacttactg
     4381 tacttgcaat gaaggatgag cttgaaaatc ctacagatga tgaaataaag cattatctta
     4441 caggaaatct atgtagatgt acagggtata tgggacagtt aagagctatc aaaacttatc
     4501 tggaggtaaa atagaacatg aatatagttg gaaaaggaat caagaaaata gatggaatga
     4561 gtatcacaac aggaaagcct gtatatacag atgatttatc agcaaaagat gctttggtgg
     4621 taaagatact tagaagtcct cacgcttatg caaaaataaa agatatagat acatcaaagg
     4681 cagaaatggt tgatggtgtt gaatgtgttt taacatataa agatgtacca aaatctcgat
     4741 ttacacttgc tggtcaaaca tatccagagc catctccata tgatagatta attcttgaag
     4801 atgttgtaag atatgtaggt gacgaagtag ctatagtggc agctatagat gaaaaaactg
     4861 caattaaagc aatgaatctt atcaaagtaa agtatgaagt acttacacct gttttggatt
     4921 ttgaagaggc aataggaaat aaaactatgg tacatgatga agatagacat tgtaactttg
     4981 atataggtat gcaaagagaa aaaaatatag tatcccatca tgattataca aaaggtgatg
     5041 tagaaaaagt attaagtaat tgtgatgtag tgattgaaga aacttattat actcaagctc
     5101 aagcacatgc aatgatggag acgtataggg catacaatta tttagaccat atgggaagat
     5161 tggttgtagt aagttcaact caaataccat ttcatgtaaa aagacattta tctcgtgcac
     5221 tagaaatacc agctagcaaa attagggtta taaaacctag aattggtggc ggatttggtg
     5281 gtaaacaaac tgcatgtgtt gagatttttt ctgcaattgt tactttaaaa acagggaaac
     5341 cagctaaaat aatttatgat agaaaagaaa cttttaattg ttcaacaagt agacatgcaa
     5401 tgagattaaa agttagacta ggtgctacaa atgatggcat aataaaggca atagatataa
     5461 aagcactttc tgatacagga gcatatggag agcatgcttc tacaactatt ggtttggttg
     5521 gagaaaaaac tttaccaatg tataataaag ttgaagcaac aagatttatt gcagatgtgg
     5581 tatatactaa taagatgcca gcaggagcac ttagagggta tggagcatca caaggatgtt
     5641 ttgctgttga atcaactata aatatgcttg ctaataaatt aaaaatggac ccaacagatt
     5701 taaggttaaa aaatgtggta acagaaggtg aaactacatt tgcatatggt aaaactttaa
     5761 atagtgtcga tttaataaaa tgtataaata aaggcaaaga acttattgga tggaatgaaa
     5821 aatatccttg taaacatctt gataatggaa aaattagaag tgtgggtatg gcaattacta
     5881 tgcaaggttc tggtatacaa ggtcttgata agtcttcagc tgaaataaaa ttgaatgact
     5941 gtggagatta tactcttatg ataggttcta ctgatatggg tacaggaagt gatacgattt
     6001 tagctcaaat ggctgctgag gttcttgata caagcattga aaatataaca gttatatcag
     6061 ctgatactga cttagttcca tatgacccag gttcatatgc ttcaagtact acttatgtaa
     6121 caggtatggc agtagttaag gcagctcaag acttaagaga aaaaataatt tctgctggag
     6181 caactaaact aggtgttgag aaggatgaag taaaatttga tggtataaaa ttaactagta
     6241 aaaaaggtag cactttatta ttcgatatag cagttgattc tacaattggt ccagatacta
     6301 atcaattaat aggtcttgct tctcatggta gcccagtatc tcctcctcct tttatagcag
     6361 gttttgcaga aactgaaatt gacacagaaa ctggaaaagt tgatttagta aattatgtag
     6421 ctgtagtaga ttgtggaacg gttataaata aaaatctagc taagatacaa gttgaaggtg
     6481 gaattgttca agggattgga atggcattat ttgaagaagt tagatacaca gaaaaaggaa
     6541 gaatggacac aaatacattt atgcaataca agattccagc tagatgtgat attggaaatg
     6601 ttaaagtaga ttttgttgaa acttatgaac caactggacc atttggtgct aaatctgttg
     6661 gagaagttgt tataaataca cctttaccag caatacaaga agctgtgttt aatggaattg
     6721 gtgtaagaat aaatacactc cctattacac ctgaaaaagt ttttatggaa atgaataaat
     6781 aattatttat gaaaatttag actagtttta catgttaaac aaccagttat gtcatacctg
     6841 ttgaaagata tttaataagt agttattata atattatgaa ttttacttat aaaatacaaa
     6901 attaaataag gggatgatgt tttggctggt aatctaaata atatgagagc agtaaataat
     6961 tttagaggag ataagaacat tttagaatgt ttagtcagct ttgagggtcg ttcaataagt
     7021 cagagaaaag taagggtatt ttttaaagaa aaacaaaatc aaatagaaat tgattttgca
     7081 gaagaggaaa tttctaaatt ggttgaaaat gttgttttaa atacatcata tcaagaaatg
     7141 ttatatgatg aaatagagaa acaactggaa attgattgta taggtacttg gatgatatta
     7201 tctaaattaa aagatggtag tagagttcac tgattttaaa ataaattatt agagcctaat
     7261 tttaaggctc ttttttgctg tttttaatta acaatagaaa acttatcttt agtttgtata
     7321 taattttaat atcatatatt ctatatatat aaaattgaat ttgtatgtat tatattgtat
     7381 aatgtaaaat gaattggaaa aatagttcga taaaataatt tgtttttata aaattactgt
     7441 ttaagtttat agattttgta taaaataaag ttatgttatt aattttttta gaaataaaac
     7501 ttttactagg agacagatat gagaaaaaat agtattaata tagcactaac tgcagcaatt
     7561 acagcaatct atgcagtttt aacattatca ttgggattta taagttatgg accaatacaa
     7621 tttagggtag cagagattat gatgcttttg gcatttttag ataaaggata tatagtaggt
     7681 cttacattag gttgtttttt agcaaatgta ataggaccat atggtgtacc tgatataatt
     7741 tttggaacat tagccacatt tataagtgca agtatgattt atattactag aaagttaggt
     7801 aaacagaata aaagtacttt agttatagct tctttatggc caactgttat aaattcgatt
     7861 gtggttggtc taatgttaaa tgtattcttt gggcttccat taattctatc tatgatacaa
     7921 gtaggttttg gagaatttgt agtagtgaca attgtaggag taccattttt ctgcttttta
     7981 atgaaaaaat ataaaaatgt cattaatatt aaattttaaa tagtgtatag taatttaatt
     8041 aattgagagt attattagtg aaaagaggtg tttatttgtc gaaggtaaaa agattgactg
     8101 atgtttggat atatattaat gataagcaaa agtttacaat aaaagagttg tcagaggaat
     8161 ttaatctttc agctaaaaca atacaacgat atttaaaaga attaaataaa atgggacttc
     8221 caattcaagc agaacaaggt agaaatggag gttatagggt gctaaataat agctatatac
     8281 ctcctgttat atttacagaa aaagaagtta tgattatact atttgcactt aagtcacttc
     8341 agatttataa ttataagtta atggaaatag aaattaattc tataatgaga aaagtaagtt
     8401 atgaacgtaa aagcagtata aaagtaggta tagaaaatat taaaagatac attggctttg
     8461 tagctaatga tactaatgat ggagatttta agagtagtaa tgtaactgag ttttttagag
     8521 cttcttcaga atctctaatt ttaaatatta agtataagtg tggtaatcag attttggaaa
     8581 aaaacgtatg ccctataggt atatatttaa ataagggagc atggttttca ccagcatatg
     8641 attgtaaaag tgataaaata atattagttc atatagataa tataattgat ataaaaagaa
     8701 caggagaaag taaaaaaata catataaact tggaagaatg gttacatgaa gtatataaca
     8761 atttatatgg aaatagttca actactaaaa aatttggtga aattatttat ctaaatgtat
     8821 tattaactaa agaaggtgtt tctaaaataa aggacagttc ttggaattta gatatggagc
     8881 taaatgaaga tggtagtgga ataattagta cattcataaa ggttgaagat atagattgtt
     8941 atgcttcgat tttatttttt attggaaata atgctaaggt tatagagcca aaatttttga
     9001 ataagctact ttatgataag gccaatgagt taagatattt ttatgaagaa aatatggtat
     9061 aatatttaga aatgttgaaa ttaaataatt attaaggaga tatacgttat aatggaaaat
     9121 tttaataaga atgctaaaat aattggtatt ggagctgaag gtattaatat aataaatgaa
     9181 gtagaagaaa aaataaaagc aaatatggat atagaaaaaa taaacataaa tcaagagatt
     9241 gaaaaagaat atgttagaag tcttttagat ggtgtagata tattattttt gatatattca
     9301 tctgaagata aacatattag agatattata aaagctgttt catatatgtc aaacgaaaga
     9361 agagttctat ctataggaat ggattgttca gagaaagaaa ataaagaaga tttagagctt
     9421 gggagagagt ttaaaatcaa taatgatagt atatttaaat ttgttgattt aatgaatatc
     9481 atggtagagt ctatttctga ttcttgcatg ataaatatag atattactga tttaaaagaa
     9541 gctattgtag gagataaagg aattaagtat tcttttgaag agtttgaaga tacaaagtca
     9601 tatagcgaga tagctgacat tttatttgac aggatggaat atattggtga ggaatttata
     9661 agtaagaaag gaattgtatt tgttgaagga agtccagaat tttctataat ggaattgaat
     9721 gatttaataa gtaatattca gagcaaggta gaagaaagct atgaagtaat attttcttta
     9781 tatataaagg aaaatttaaa tggaaatatt agagtaggct tattgtataa ttagtatatt
     9841 ttagatatta aggagagtgt atatgtctga aaatataaga ggtacagagt attggttaat
     9901 ggaagaattt ttttatcagg aaacagacac accacagatt tttagagaag aaagcataat
     9961 tgcacttgga tttgacataa aaataaattt aaaaaaagta ttcagtatgg ggaaaaaatc
    10021 agaaaagtat attaatgaga attgtaatga taaagatttt ataaaaaagt ttatgtcgat
    10081 taaaaaagga gattattttt tatataaagc atatcaaaat atagaagaaa aagttaaatg
    10141 taatgtgtct attggcatag ttgaagaaga ttttgaaaat ggatatgaat taagtcatat
    10201 tttagggcat acactaccag taaaatggat gcataatttt gatacagttt caaattgtaa
    10261 agtatataca agtgaatttt ataaattaaa taataatgat attagctcat atttagaaat
    10321 tattaagtca caagataaga aattattgag aaaaagaata gaagaaagtg aaaataataa
    10381 atggtattta aaaaactatt attataaaaa tgtagataag tgtgtagatg atgaagaatt
    10441 atttattcag cccattaatc tagaacaatt agaaagccta aaaaatataa agacagatga
    10501 ttatatttta ttgtataata gcgagaaagt aatttgtgaa aatgaccata caatgaagag
    10561 tctatttggc gtatgtcaag ttatggaagg gttcgataaa gagaaagtaa gtgctttgga
    10621 aggtggatat ttttttcctg ttagatggat agagtatgaa aaggagaata tagacggaaa
    10681 tgcatgggtt atatttgaaa ataatagtat agttgaaaag tatgtaaaaa gatttactaa
    10741 taaaatgcca ttaaatacta tactttatgg accaccagga actggtaaaa catacaatac
    10801 aaaacagatg gtttacaatc tagtaagtga aaccagtcta agaaacaaat actatacagg
    10861 ttacttagat tataaaaaca atgtagaatt ttgtacattt catcaatcat atggatatga
    10921 ggaatttata gagggtctta agtctgatgg tgaaggcaat tttaagcaag aagatgggat
    10981 attaaaggat atatctatag aagcatgtta taatgcattg aaatttgaaa aaaagatgag
    11041 ctttgagttg gaaaaagata gcttgacttt agaaactata aaagacagga aaaagaaatt
    11101 agtattagaa aatatagaat atagtgagag ttttaatttt gaagaatcag aaaactatgt
    11161 tttaataata gatgaaatta atagagggaa tgtatctaaa atatttggtg agctaataac
    11221 attattggaa gatgataaaa gacttactaa atcaaatcaa actattgtca aactaccata
    11281 ttcaaaggaa aaattttctc tacctccaaa tttgtatata gttggaacta tgaacacatc
    11341 agataaatca atagcacttt tagatatagc tttaagaaga aggtttaatt ttgaagaaat
    11401 tatgccagat tatgatttat tgtctattgt agatggtata gatattagaa aaatgctata
    11461 tacaatcaat aaaaggatag agtttttata tgatagagat catgttattg gacaagctta
    11521 tctacttgct gtagaaaata ttaatgatat agtagtagta tttagaagta aaataatacc
    11581 acttcttcaa gagtattttt attatgattc agaaaaagta ggtttgattt taggaggaat
    11641 tggaagaaat gaaaaagata aatacattgt ttataaagaa aaacttatag cttctgagtt
    11701 atttaaaaat aataatgcct caataatgga gagtaaagtt aactattgta taaaacaaaa
    11761 catatctagt gaggagctaa aaaatatata tgagtaaaaa tatatatgtt aaggaaacct
    11821 atgaatggat aagggtaggt aatggtgaaa atgagcttac agaaattgaa tatgaaaaat
    11881 tgcttaaata tctagagaac aataatgatg tattaaaaag taatataata gatattaaat
    11941 acaaaaaact tagatttata aactatgtag gcatcatatg ttttgaaaat gtaatacttg
    12001 aaatacttcc taaattgtca ttaagtgata atcttgtaaa agatagagag atactattac
    12061 agatgctttc tatatgtaat aaaattccaa ttacaatgaa tgaaaaaata agattgagtt
    12121 tgaaaaatta taatctctta aatttttttg ttatgtattt cattgagagt atgcaaacac
    12181 agatgaaaaa aggtatttat tttgaatata taaataaaat agagaactta aatgttatga
    12241 ggggtaaaat attattaagt acatatgcaa aagaaaaagg catttctcca atgaaaataa
    12301 gatgtgaata tgatgagtat agtgaaaata atttcttgaa tcaagttctt aaaaaagcat
    12361 gtatatctat tttatgtaga ataaatgaca attcaattca aggcaagatt aaaaaaattt
    12421 taagctattt tcaaaatgta gatttaattt atatagatag aaaaaaacta ttggattata
    12481 aattttataa aaacaatgat agatttaaag attgttattt actagctaga cttatacttt
    12541 taaatttgtc tatggataat agtcaaaata atcaagaggc attttctata ttatttgaaa
    12601 taaatacttt gtatgaagaa tatataggaa ttttaatcaa aagtatatgg gacaattcat
    12661 ttagagaaac atatatacaa gataagagta aatttctttt gaaaaatgaa caaacaggta
    12721 agaaaaactt taatttaaga ccggatatag ttttatatga cttaaaaaat gaatatgaaa
    12781 ttataattga cactaaatgg aaagctattg aagtagattc aaatgttttt tataggtcaa
    12841 gtgatatata ccagatgtat gcatatataa cagcatatga aaatgcaaaa agatgtatat
    12901 tattatatcc ttgtattcaa aaagataaga attatagttc ctggaaatta tcagaatcat
    12961 ttaagggtaa gtttatagaa gcaaaaactg tgaggttaga tgatataaaa aacacaaaaa
    13021 atgatttaaa aaagataatt tttaattata aattttaaaa ataagaattt taaaatttat
    13081 aaaagtaatt ttatataggt ataaaaatta cgagtagtat aaaaaattta ataagaactt
    13141 aaattaaaaa ttttaaaaat tatttccata taaaatatgg cattgtaatt gcttatttaa
    13201 tagagcattt ttgtaaaatg aagaaagtat atctatcaaa ttaaaactaa gtagatatac
    13261 tttttattaa gttttgtttt aaaataatat tattaaatta taactaagct tttctgttat
    13321 gatataatta aatagataat gtgaatgatt attatataga atacttattt ggagttgata
    13381 ttttatggac aaacacagtg attcagtaat gtatggtgga atggaagtat ataatatttg
    13441 gggaacaaca acagtagact ccataattgg agagtctgat gtaatgcgtg ctcttaagaa
    13501 tagaatttgt aaaattggaa atagtgattc tactgtagct ataactggag aaagtggcac
    13561 tggaaaagaa ttaatagcaa gagccattca tctcgcagga aacagaggga ataaaacatt
    13621 cgttgctata aactgtgctg ctataccaga acctttacta gaaagtgaac tttttggata
    13681 tacaaaagga gcttttagtg gagcagattc aaaaggtaaa attggtaagt ttgaattggc
    13741 caatggaggg gttgtatttt tagatgagat aggagatatg ccaattcaac tacaatcaaa
    13801 gttattgagg gtattacagg aaaagaagtt tacgagaata ggttcaaatg aactaattga
    13861 tatagatgtt agaatcatat cagctactaa tagagattta tttgagttgg ttaaggaaaa
    13921 taaatttaga gaagacttat actatagatt gaatgtaata cctttagaag tgcctccact
    13981 tagagaaaga ggagacgata tacaaatatt aataaacaat tttatagaca aatattcgag
    14041 aaagatgaag aaaaacgtgt accatataga gccagatgtg gaacaaatgt tgcttaagta
    14101 tccttggcca ggaaatatta gagagttgga aaatactata gaactggcat taaaccttct
    14161 tgaggaagat ggaataatac ataaaggaat aataaataga aagatacttg aatattttaa
    14221 gttaaataat ataaatataa agttacttga ggaaagtgac tatgagataa gtatggaaca
    14281 agacatactt acacttgaag agtttgaaag agcctatata aagaaagtgt taaagtttta
    14341 cggaaatgat actaagtcta aaaaactggc tgccaaaaaa cttggaatat cactagctac
    14401 attgtacaga aaaatagatg tataataatt aggcttttta ttctcaattc taaaatttga
    14461 aattataaaa ttgataaaaa acacttggaa aactattttc taagtgtttt tttgtgtaat
    14521 agcattttat tatccaaaaa taaaaaaaat ttaaaagaat ttaaaaaaat aaatgcttta
    14581 ataaattatc aaatactaac aaatataata aaaattatat atgtaaaaca attatgttaa
    14641 aaaattggca ttataattgc ttcatattaa gttgagatga caaaataaaa aaagaaagga
    14701 tgttgtgtga gataataata actgtcatta aaaaatagtt gggagtgata accttgagta
    14761 aatatcaaaa gaatgtttct atgtttgata ttgatggaag gccaccaatt ttaaagtgtt
    14821 tgccactatc aatgcaacat atactagcaa tgatagttgg aactgttaca gtacctatag
    14881 tcgtaggagg agctgtgggt gttagcccta cacaaataac acttttagtg caatatgcat
    14941 taataatagc tggtatatca acacttatac aactatatcc aataggaaat attggttcaa
    15001 gattgcctat aatttttgga gttggtttta cttatgtacc aacacttgta gcagtcggag
    15061 gaaattatgg attagcaagc attttgggtg cacaattaat agggggagtg gctacagtat
    15121 taatagggat attcataaaa aagataagaa aattttttcc gaatatagtt gcaggaacag
    15181 tggtttttac aattggatta tctttgtatc ctatagcaat aaattatatg gcaggtggtg
    15241 taaatagtcc tacttatgcc tctatgcaaa actgggcagt agcggctata acattagtgg
    15301 tagttatagg tcttagtcaa tttgcaaaag gatatgcaaa attggcagca atatttttgg
    15361 gaattatagt tggatattgt gtatctttag ttctgggtat aataaatttt gaccctatta
    15421 gagaggcaac ttggtttgcg atacctaaac cgcttgaatt tgggatggaa tttgatatgc
    15481 ctgtaataat ctcagtagta attgtaagta taatcaatgc tgttcaagca attggagact
    15541 tatcagctac tacaatggga ggcatggata ggaatatcac agataaggaa ttatcaagtg
    15601 gagtaatagg aagtggaatt tgtactatga taggagcttt gtttggaggt ttaccaccat
    15661 catcatatag ccaaaacgtg gggatggtag ctatgaacaa agtaataagt agatttgtgc
    15721 ttggaatagc tggtatattt atgttattgt cggggtttgt gcctaaattt ggtgcattaa
    15781 tgactactat accatattct gttttaggag gagctacaat atctatattt agtataataa
    15841 caatgacagg tataaagtta attattacag aagagttgtc atcaagaaat attaccattg
    15901 ttggtttggc aattgcctta ggcatgggaa taacatcagt cccagcagcc caagaggcat
    15961 ttccttcatt tgtaaagtta gtatttggag aatctccaat agtaattgca acattggttg
    16021 cattcgtttt aaatctaata ataccaaata aaagtctaga ggatgaggct aaagaaagag
    16081 ctgttatgga agagactaat taagatataa taatttaaaa gaattgttta aattatttag
    16141 tatgagattg ttagattatt tttgatagct attcatttta ataaaaaatt taacaaataa
    16201 atatatacca ggcaattaat gttcatttaa gttgaaaatc ggggaggcaa tatttagaaa
    16261 tttgttaatt ttttataaaa atagctcagt ttagctgtgt ttatcggttg agcaataaat
    16321 tcatggtcat aaaaatattt atagaaaata attataaaaa tatttagtca agatatttat
    16381 gtatgggagg atttatttta tgaaaacacg taatacttca aaatatgatg tcgatgggat
    16441 accaccacta aaggaatcaa tacctttggc cttacaacat ctgttagcaa tgatagtagg
    16501 aaatatggta cctgcgatat taatagcaaa cgtggtagga cttaaccagg ggcaagcaac
    16561 aatgttgatt cagggttcta tgttagcagc aggtttagca acgtttttac agttatatcc
    16621 aataccttta tttaaaggat ttaagttagg ttcaagatta cctgtaatga tgggaatgag
    16681 ttatgtattt ttaggagctt gtctttcagt agcagctaaa gatggtttag cagctctatt
    16741 tggagcacag attgctgctg gggtaatcgt attctttgta ggatttggag ttaagaaaat
    16801 tagacatata ttcactccaa tagtttctgg aactataata gcatgtatgg gacttggact
    16861 attcgcaaca gctataaaga atttagctgg aggagaaggc acacaaacat ttggaagccc
    16921 aataaacttc acagtaggag ttatagttgc ttttgtaata ataatgataa ataaatacgg
    16981 taaaggttta gtaaaaaatt cttcaatatt aataggaata ttagtaggtt atgctatatc
    17041 tttagtttta ggattggttg atttctcagc cgttcaagga gcagctatag tatctttacc
    17101 aacaccagca gcttttggat tagaatttag accagagtta atagtaatgt ttactattat
    17161 ttacataata ggtatagctg atatgatggg tgcctgtaca atagctacag taggagcaat
    17221 ggatagagag gtaactgacg aagaattagc atcagttgta ttaggaaatt ctataacttc
    17281 tataatttca tcactatttg cagctcttcc aactggagta tttagccaaa atacagcaat
    17341 tgtatctatg aacaaagtta caagtagatt tgtaatagca ctaggggcac ttgtattatt
    17401 actagcaggg atatctccag cattgggtgc aataatgaca actataccat cttgtgttgt
    17461 tggtggagca actttagtag ttttctcatc aatagcaatg tctggtttct ctattatgag
    17521 tatggatgga ttcactgaag aaaacaatct tatagcaggt gtatctatag caacaagtat
    17581 gggtcttaca actgctccac aagttttaga ccaattccca gaaacaattg gaactgtcct
    17641 tggagggtca agtatagttt ctggagcaat aatagctata ttacttcaaa cattatttaa
    17701 attaaagtca cgtaaaagtg ctgaaaacgt tacttcaaat cttgaagaaa atattggata
    17761 aatgtattag atataatatg agaaaaccat atggtaaaat gttgtaatga aagtattcaa
    17821 agggggattt aagatgattg atatactaat aaaaaatgca ataattgtta cagttaataa
    17881 agacagagat gttatatttg atggagcaat agctattcaa ggtgacagaa tcttggacat
    17941 aggaaataca gttgatatcg agcctaagta tcttgatgct aaaaaagtta tagatgctaa
    18001 tggaaaagta atattccctg gctttataaa tactcataat cacctgttcc aagtgttatt
    18061 aaaaggttta ggtgatgata tggcacttca tgagtggtta aatacaatga tgtttccatc
    18121 agcaaaattc ttaacagaac aagatactta tgatgctgca atgcttggat gtatggaagg
    18181 tttaaaaagt ggtataacta caatggttga ttatatgcat actcacaata gaccaggact
    18241 tacagatgga atagtaaagg catataaaga tttaggcata agaggggttg taggtagagg
    18301 ttgtatagat ttaggtattc acaaagagct tatagaagat gtggaaacgg ttgaaaaaga
    18361 tttgagaagg gtattcgaaa aatatcataa ttcagaaaat ggaagaataa aaatctgtgt
    18421 agctccttct tcaatgtggg ctatatctga agaaatgggt aagatgcttt ggaatatagt
    18481 taaagaatat gattcatatt ttacagttca tatatctgaa acagaatttg caagaacagc
    18541 aacaaaggat atacatggag aaatagatat aaaattactt gaaaaatggg gtatagtagg
    18601 tccagaagtt gtagcagtac attgtgtatg tataactgat gaagacatgg aaatgcttaa
    18661 aaagtatgac ataaaagttt ctcataatgt agcaagtaat atgtatcttg cttctggtgt
    18721 tgcacctgtg ccagaaatgt taaaaaaagg tataaatgtg agtttaggtc ttgacggtgc
    18781 agcaagtaat aacgcacaag atatggtaga gcttatgaaa ctaacagctt tacaacacaa
    18841 agtaaataat tgtgaccctc tggcaatttc tgcagagaaa gttcttgaaa tggctacaat
    18901 tgagggagca agaactctta gaatggaaga tgaaataggt tctttggaga ttgggaaaaa
    18961 agctgaccta gtaatattta actcaatgct ttctccaaaa tcaatacctc ttcacaatcc
    19021 tgtatcaact ttggtatact cagctagtat gcacaatgta gagagtgttt tagttgatgg
    19081 aaatgtaata cttgaaaatg gaaaagtaac tacaattgaa agtgaagaac aagtgttgct
    19141 taatgcacaa catgcagcag aagaattatg taagagagct aatgtaacta atagaatgga
    19201 aggacataag tggaatagct tatactaata ttaaaataag attgtaataa tattctatta
    19261 caacattagt ttatctgtag gaggagaaaa tggaaaaaat aaaattaaat attaatgatt
    19321 acatgtatga aatagaagtt gaaaagaatg aaactctttt gcatgtactt cgtgaaaaat
    19381 taaatctaac tggagcgaaa caaggttgta atagtggcag ttgtggagca tgtaaagttt
    19441 tgatagatgg agttgacatg aaatcttgta aacttcttgc aaaaaactgc gaaggtaaag
    19501 ttatatacac tatagaaagt tttgctaatg gagaaaatct tcatccaata caagaagctt
    19561 ttatagaagt tggagctgta caatgtggat attgtactcc tggtatgatt ataacaacac
    19621 aagctctttt aaatagaaat gcatctccta atgaagatga aattagagaa gcttttaaag
    19681 aaaatctctg tagatgcaca ggatatgtaa agattataga agctgtaaaa ttatctgcag
    19741 ctagattagg gggtagataa ttatggaaaa ggtaatagga aaatcatacc ctctaaaaga
    19801 tgcagcttta aaagttactg gtcaaatgaa atacgtggct gatatgaaac cacaaaatgt
    19861 actttacgct aagatgcttt taagccctgt agcacatgca aaaataaaaa gtatagatac
    19921 aagtgaagct gagaaagtag taggtgttag agctatagct acttatctca atacatcaca
    19981 aagaaaatac aatggttcat taagatttta tgagcacaaa atacctgaaa atgaagttat
    20041 attttcagat acagttagat ttgttggtga ccgtgttgca gcagtagctg cagagactcc
    20101 agaaatagca gagaaagctg taaaacttat aaaagttgaa tatgaagaat tgccagctgt
    20161 atttactata caagatgcaa taaaagaaga tgctatctgc atacatggag attcaaatat
    20221 agtttctcaa aacaacataa atgggggaga tgtagaaaaa ggcttcaagg aatgtgacta
    20281 tatatttgaa gatgaatact ctactccagc aatacatcat agtgcaatag agactcatag
    20341 tgtaattgca cactatgatt ataatgataa attaactgta catacacctt gccaaaatac
    20401 atttggattt agaataatac tatctcaaat ttttgggatg tcattaaata aagtaagagt
    20461 tataagacct gctattggag gctcatttgg tggaaaatta gaacttacaa ttgaaccagt
    20521 agcagccgca ttatcaaaaa tgacaagaag accagttaaa atggttctta caagaaagga
    20581 atctatgata tcaacaagaa ctcgtcatgg gtcttttgta aaattaaaaa ctggtgtaaa
    20641 taaagatggt gaagtaatag cacaagacat aaaaatatat acaaatacag gagcatatgc
    20701 atcaagtgct ttaaatgtta taggagcact tagtcataaa gtatttaaag tttataaaat
    20761 accaaatata aagtttactg ggatgcctgt atatacaaat acaccaatag caggagctat
    20821 gagaggctat gggtcacctc aaatatttat ggctcaacaa gctcaatttg cgaaaatagc
    20881 aaaggaaata ggtatggatt tagtcgattt tcaaaataaa aatgcagtag agccagatga
    20941 tgtcgatata atcttccacg gttctcttgg aaatcctaga gtattggatt gtatagagca
    21001 aggtaagaag atgtttaagt gggatgaaaa gaaaaaactt ccaaaagaag aaggaaaata
    21061 tttaagaggt ataggtatgg caataggtgc acatggtaat ggagtttttg gtgcacatag
    21121 agatgttgtt acattgactc ttaaattaaa tgaagatggt acgttgacac tgctaacagg
    21181 agctcatgat atgggtaatg gtgttgtaac tatgcaaaca atgatggtag ctgaagtttt
    21241 aggaataaca cctgataaag ttgatacatt tgaaactgat acggatgcat gtacattcaa
    21301 ccttggtgac tatgcaagta gaggggtatt tgtagaaggt ggaggagcta aaaagacagc
    21361 agagaaattg aaaaatctta tattagaaga agcagaaaaa ttacttgaaa catcaaaaga
    21421 agatttatat ctagacaatg gatgtgtaat tagtaaactt gatgaaaatg taaaagcatc
    21481 actttctgat gtcgcagtat atagtcaaag taaatcatta agagagttaa ctgttgttga
    21541 agattattct tcaccagctg gacgtacttc ttatggtgta cattttgctg aagttttagt
    21601 agataaagaa actaaggata taaaagtagt tgattttgta gctgtacatg atgttggtag
    21661 agttataaat cctctaaatt tagaaggaca attagaaggt gggattcata tgggtctagg
    21721 atacgcattg agtgaagaat tggcatttga tgaacaaggt agacctaaag ctactgattt
    21781 taaaacatat aaattattta gaactgtaga tatgccaaaa tgtcaagttg catttgtaga
    21841 tggttatgaa aaagctgggc cttttggtgg taagagtatt ggagaatgtg ccgttgtacc
    21901 agttgcgcca gctgttgcaa atgcagtata taatgcaact gatgtagaga tacattcatt
    21961 accaataaaa ttaaaatagt ataaattaaa gcagaaataa caatttattg tatttgtaaa
    22021 tagataataa aaatagattt atttggtgag gagatattat tatggcaaag atattaggta
    22081 tttgtggaag tccacgtaaa ggagcaacag aatatgcagt gttagaagct ttgaaagaag
    22141 cagaaactat acctggtata gaaactgagt attggtcagt tagagggaaa aaaataagtc
    22201 cttgtgtaca ttgtgatgca tgtataagaa aagaaactat gtgtattata aaagatgata
    22261 tccaagaatt agagcaaaaa atattggagg cagatggaat tatagtaggt tctccagttt
    22321 atgatatgaa tataactgct caacttacag cagtatttaa tagacttcgt ccaatgtatt
    22381 tagttcatcc aggaaaactt caaaataagg ttggtgcagc tataactact ggtggaacta
    22441 gatatggtgg ccaagaattg acaaaacttc ctataataaa tttcttccta atgcatgaaa
    22501 tgttagtgtc tggaggttta ggtggatgct atataggtgg tacaatatgg agtaaagatg
    22561 gaaaagcaaa aggtgcccaa gaggatgaga caggtatgga cacagtaaaa agacttggaa
    22621 aaggtgcagc agaggcagtt atggtttcta agtttggtct tgaaaaatgg aatgtcgtaa
    22681 aagaagaatt agatgttaaa aaagatgaaa aatcacctct tagagaccac taaagattta
    22741 ttatattatt gaatatatac aaatagtgat gtaatagatt attgcatcac tattttatgt
    22801 tttaatttag attttataga ttttatatta aaaaatatag aaacaaagtg ttttgagatt
    22861 tatttttaaa ttttaaaaat gagaattaaa aagtgagaag atagaaaagg ttttttagtt
    22921 attaaattct acatacattg tatgaactaa agatagaaaa gaaaaaataa taaaagcatt
    22981 gcaatttcta tactaaagag tcatagaaaa atatctagta tatatttagt gttggcatgt
    23041 taattgctta tatattaaag tataaaaatt taacaataat ttggaggtat tttagatgtt
    23101 aaaagaaatt aaatggaaag taaataattt gccaaagggc gataaggaaa attgtatcaa
    23161 atttttaaat gaagaagaaa taactaaggt tagaaatttt cacaaaagct ttcctcaata
    23221 taaagaaact ccattagcaa acctagaagg tttagcaaaa aaattaggtg ttgcaggagt
    23281 atatgtaaaa gatgaatctt atagatttgg attaaatgca tttaaagtat taggtggttc
    23341 ttattctatg ggtagatact tagctcaaag attagataca gatatatctg agttagggta
    23401 tgacaaatta acttcaaaag aaataaaaga aaaattagga gaaataactt tctttacagc
    23461 tacagatgga aatcacggta gaggagttgc ttggacagct aataaattag gtcaaaaatc
    23521 tgttgtatta atgccaaaag gttcttctga atttagatta aataaaatta aaggagaagg
    23581 tgcagatgct agtataacag acttaaacta tgatgatgca gtaagattag ctaatgatta
    23641 tgcagaagct gatgaccatg gagtaatggt tcaagataca gcatgggacg gatatgaaga
    23701 aataccagcg tggataatgc aaggatatgg aactatggct caagaagcta tagagcaatt
    23761 aaaagaatat ggagtagata gaccaacaca cgtatttgtt caagcaggag ttggttcact
    23821 tgctggtgca gttcaagggt atgttgcttc aatatatgat gaatgcccaa taacagtagt
    23881 agtagaggct gatgaagctg attgttacta taaatcagca gaagctggag atggaaaacc
    23941 aagatttgtt ggaggagata tgccaactat aatggcaggg cttgcttgtg gagagccaaa
    24001 tactatagga tttgaagtat taaaaaatca tgcagcagca tttgtttcag caccagactg
    24061 ggtttctgct aagggtatga gaacattagg taatccttta aatggtgatg aaaaggtaat
    24121 ttctggagaa tctggagcag ttacaactgg tcttttagta gcagctatgg aaagagaaga
    24181 tttagcagat ttaagaaaag atttaaaatt agatgaaaat tcaagaatac ttttaataag
    24241 cacagaagga gatactgacc ctgacaaata tagaagtata gtttgggatg gagaatatcc
    24301 aagtatataa ggaattacta aaatgcaatt attagatatt ggtttacata tttagaagat
    24361 atataagttc taaaatttag aaaatatata agtatataaa atacatacat tataaaaaat
    24421 ctagggggat aatgaaatgt taaacgaaca aagaaaacaa gaagtaactg aaatatgcca
    24481 aaaattaatt caaaatccta gttcttctgg aaatgaagaa ggtgttgtaa aagcaataga
    24541 agagtctttt gaaaaattag gttttgatag ttggtcaaga gatagatatg gaaatatagt
    24601 aggatgtata aaaggtaaca agccaggtaa gaaaatatta tttgatggac atatagatac
    24661 agttccagtt ccagatgcaa gtaaatggag tgttcctcct tttgaaggaa aaatagttga
    24721 tggaaagata tatggtagag gaacttctga catgaaaggt caatatactg caatgatgtc
    24781 tgctgtagcg tattttgcta aagacactaa tagagatttt gctggagaac tatatgtagc
    24841 aggtgttgtt catgaagaaa tatttgaagg tgtttcggct agagagataa gtaaggctgt
    24901 tcaacctgac tatgttgtaa taggtgagtc ttcagagtta aacttaaaga ttggtcagag
    24961 aggtagagga gaaatagtag tagaaacttt tggtaaacca gctcactctg ctaatccaga
    25021 aaaaggtgta aatgcagttt ataaaatggc taatgtaatc caaaagatac aacaattagt
    25081 acctccaact catccagttt taggtgatgg aatattagtt ttaacagata taaaatcttc
    25141 tccatatcca ggagcatcag tagtgccaga ttattgcaaa gctacttttg atagaagatt
    25201 attagttgga gaaacaagag aaggcgtatt agctccaata caagctttat tagatgaaat
    25261 gatgaaagaa gacccagaat taaatgcaaa agtttcttat gcagttgaaa aagctgattg
    25321 ctatacagga aatactatag aaagtgaaag attcttccca ggttggttat atgacgaaga
    25381 agatgaattt gttcaagcag catataaggg attaaaagaa gctggaatag attcagaaat
    25441 aactcaatac tcattctgta cgaatggaag tcactatgca ggtgaagctg gaataaaaac
    25501 tataggattt ggtccttcta aggaaaatct tgctcacaca atagatgagt atatagagca
    25561 agaacagtta tttataggaa cagaaggtta ctatggaata ttaaaatctg tatacggaaa
    25621 ataatattta gttcacaata aattatttaa atatagtcta gaggtgctga atatagcacc
    25681 tttagaaaat acaattttaa ataaagtttt acaatactta gaaaatactt taaagcttaa
    25741 aagttaggag gtatatatgg gaacaatact aagaggtgga actataatta cctctgataa
    25801 gacttatata agtgatttaa ggatagaaga tgaagagata gttgaaatag gaaataactt
    25861 agaaataaat ggtgataagg ttattgatgc tacaggtaaa attgtaattc ctggtggaat
    25921 agacacacat actcattttg atatgaatgc aggttctatt acaactgcag ataattttga
    25981 aacaggaaca agagcagcta tagctggagg aactactaca atactagatt ttgcagaagc
    26041 caatgagggg gaaaacctac tgcaaggtgt agaagcatat cataaaaaag caagtggaaa
    26101 ttgttattgt gattatggtt ttcatatgac aattacatgt ttaaataaag atacatttaa
    26161 tcatatggaa aaacttattt cggatgggat agtatctttt aagatgtata tggcatatga
    26221 tggcatgaaa gtagatgatg gaacaatata cagggtttta aagaaagcaa gggatttagg
    26281 ttgtatagtt ggatttcatt gtgaaaatgg ggatttacta gatgtattga tagaagaaaa
    26341 tatacaaaat ggtaatttgg agccaaaata tcatccactt acaagaccta acatagttga
    26401 gaaagagtct gtttcaagac ttgctgatat aactaaatta tctaattcaa aatcctatgt
    26461 agtccactta agttgtaggg agtcacttga aactgtaaaa atggctagag aaaaaaatat
    26521 agatatgata gtagaaacat gcccacagta tctattatta gaagacaatt tatataataa
    26581 ggataaattt gagggtgcta agtatgtaat gtctcctcct ttaagaaaaa aagaagacat
    26641 aaattacctt tgggaaggat tgtctagtgg agatattcaa acagttggca cagatcactg
    26701 ttcatttaat tttaaggggc aaaaagactt gggaattgat gattttagta agataccaaa
    26761 tggagcacct ggtgttgaac ataggttagc acttttatat acatatggag tcttagaaaa
    26821 tagaataagt gcaaataagt ttgtggaagt tacttctacg aatgctgcaa aaatatttgg
    26881 aatgtatcct aaaaaaggtg agatagctgt aggaagtgat gcagatatag tcattttaaa
    26941 tatcaataaa gaagaaacaa ttagttataa aactcaaaaa caaaatgtag attatactcc
    27001 atatgaagga tttaaggtta aatgtaaagt tgaagatgta tttttgagag gaaatcatgt
    27061 agtacaaagt tgtaatgtta aagaacatcc aacaggtcaa tatattaaaa gaaaaattac
    27121 taaataatgc ggggggaaat aagaatgatg gatatactta ttaagaatgc cataatagta
    27181 actgtgaata aagagagaga agttattttt gatggggcat tagtagtaaa agataacaag
    27241 attgctgata ttggaaattc taaagagata gaaagtaaat atactgatgt taaaaaaatt
    27301 atagatgcta aaggtaaagt tttattccca ggttttataa atacacataa tcatttattc
    27361 caaactttat taaaaggatt gggagatgat atggtactta aagactggtt agaaactatg
    27421 acatttccag cagctaacta tttagagcca aaagatactt atgatgctgc aatgcttgga
    27481 tgtattgaag gacttagaag tggtataaca acaatggttg actacatgta tcctcatagt
    27541 aagccaggtc tttgtgatgg aataatagat gcttataaag aattaggaat aagaggaata
    27601 cttggaagag gatgtatgaa tacaggagct caatttggag ttcatcctgg aataatgcaa
    27661 gatgtagaaa cggttgaaaa agatgtaaga agattgtttg aaaaacacca taatacagaa
    27721 aatggaagaa tcaaaatagg agtagctcct gcagcaatat ggtcaaattc tcaagaaatg
    27781 cttgaaatgc tatggagagt agtaaaagaa tatgatgatg ctttatttac agtacatata
    27841 tcagagactc cttttgatag agaagcagca aaagaattgc atgggcaata tgacatagat
    27901 gttttagaaa aattaggaat acttggacca aatgtgctta tggtacattg tgtgtatcta
    27961 actgaaaaag atatggaact tactaaaaaa tacgatatga aagtgtctca taatacagca
    28021 agtaatatgt acttatcttc aggtgttgca ccagtgccag aaatgcttaa aaaaggaata
    28081 acagttagtt taggagtaga tggagccgct agtaataact ctcaagatat gcttgaactt
    28141 atgaaattaa cagcacttca acataaagtt aataagtgtg acccattagc aatgtcagca
    28201 gaaaaagttc ttgagttggc aactatagat ggagcaagag ctattggaat ggaagatgag
    28261 ataggttcat tggagatagg taaaaaagca gacttattaa tatttaatcc aatgctttca
    28321 ccaaaagcga tacctatgca taatccggta tctacattag tatattcttc tagtatgaaa
    28381 aatatagaga gtgttatagt agatggaaat ataataatgg aagattcaaa aatattgaca
    28441 gctaatgaag agaaagcatt aaaagatgct caagatacag ctgagagact ttgtgtaaga
    28501 ggaactataa aaaatagaat ggaaggtcat aaatggaata gcctttatta ataaatgtaa
    28561 tttattcata aaaaagtata gtaaaacttt agcttaaaaa tatgagtttt gaggtgattt
    28621 atttgattat caaatgtacc ataaataata aagaaaaaga attaagtata agaccagatg
    28681 attttttagc agatacatta agagaaaatg gatatgtaag tgttaaaaaa gggtgtgata
    28741 caggctcatg tgggttatgt acagtgatgg taaatgacaa ggccgtgctt tcatgtaatt
    28801 atcttactgc tagagtagaa ggtcagcata ttacaacact tgagggtgtc ttggatgaag
    28861 caaagaagtt tggagatttt cttgcagacg aaggggctga ccagtgtgga ttttgtgctc
    28921 ctggatttat aatgatggtc ttggcaatga aaagagagct tcaaaatcca acagaagaag
    28981 aagttataga ttatgtaaat ggtaaccttt gtagatgtac aggatataaa ggtcaattaa
    29041 gagcaataca taaatattta aacacttaga cttttaagtg aaaattaagt tgatatgggg
    29101 agagagtatg ataactataa aggaatatgt agttccaaaa agtttagatg aagcttatga
    29161 actgctaata tcaagaaaaa acaacataat acttggagga tgtggatttt taaaactagg
    29221 tagtaaaaat attggttcag caatagattt aaaagactta gctttagatt atataaatga
    29281 aactgatgat agcatattaa ttggagcaga tacaagttta agaactttag aattaaataa
    29341 ggttattaaa aattactgta atggggtaat ttcaaacgct gtttctaata tagtaggagt
    29401 tcaatttaga agtggagcta gagtaggagc tagcgtattt gctaagtatg gattttcaga
    29461 tttaatacct tcattactag tggtagatgc aaaggttaag ttatataaaa aaggggttat
    29521 gaacttagct gattttttag aaaatgagct tgaaaaagat atattaatag aagttatatt
    29581 acctaaaaaa gatgctatag gtgtatttga ttctataaga aaatgtacag gtgattttgc
    29641 agtattaaat ggagcaatgt taaaagaagg aagtatttat aaaattgcga taggcgctag
    29701 accaagaaga gcaaaaattg cctataaagc aagtgaaata ttaagtttag aaaatgatat
    29761 agaaaaagct ggtgaagtat caagtgaaga actttcattt ggaagtaata taagaggaag
    29821 taaagaatac agaaaagata tggcaaaagc cttagtagtg agaatgtata atagtattgg
    29881 aggtgagtgt gatggtaaat aaagaggtaa gaaaaataga tagtgaagcc attgttactg
    29941 gaaaaccaat atatacagaa gatttaattt ttcataaaga tgttttaaca attaagcttc
    30001 ttagaagtcc acatgctttt gcgaagataa aaaacataga tataaaaaat gcattaaagg
    30061 taaaaggtgt agtaaacata ttcacttaca aagatgttcc aaattataga tattcaatgg
    30121 ttggagagtc atatccagaa gcatcacctc atgaccaatt aatacttgaa gacgtagtta
    30181 gatatgtagg agatgaagtt gctataatag ccgctgaaga tgaaagggct gctattaaag
    30241 ctatgaaact tataaaagtt gaatatgaag taatgactcc tatattggat gcaagagaat
    30301 cggaaggaaa taaaatttta atccatgaag aagatgattt gttttgtcca tttgattttg
    30361 gattggatgc tacaaaaaat attgtatcga gagaaaaaat gggtaaaggg gatgtagaaa
    30421 aagagtttaa agaaagtgat gtaatagtag aacattctta tatgactcaa tcacaagccc
    30481 attgtatgat ggaaactcat agagcttata cgtatataga tacgaatggc agattagtag
    30541 taacttctgc aaaccaatca gcttatcata tgagaagaca ggtttctaaa gcactgggaa
    30601 tacctctttc aaaagtgaga gtaataaaac caagaatagg tggaggtttt ggtggaaaaa
    30661 acatagctgt aacagagata tatgcatcat ttgtaacttg gatgacaaaa agaccagcaa
    30721 aactaattta tactagagaa gaaacttttg caatgactaa tactagacat cagatgtttt
    30781 ttgatataaa aattgggtca gataaagaag gaaacataaa agctatagat atgaaagcat
    30841 tgaacaatac aggcgcttat ggcgataatg gaccttcagt ttgctctgaa tcaggtcata
    30901 atgtacttcc aacatataat aatgtacctg caataaaatt tgatgggaga actgtatata
    30961 ctaatttagt tccaggagga gcattaagag gatatggagc aacacaaggt acatttgcac
    31021 ttgattgtgc aattgatgaa ttagcgcata aacttaatat ggacccaaca gaaataaagt
    31081 taaagaatat tataaaaaaa caaactgaag gcggaataat gaattttcca ataagaagtt
    31141 gtgcacttga tgaatgtata attagaggta aaaaacttat tggatgggat gaaaagtacc
    31201 ctgttaggga tattggaaac aataaggtga gggctgttgg aatgtctgtg ggaactcatg
    31261 gttctggtat agccaacata gacatggcaa tagtaaatat gaggatggat gaagatggaa
    31321 cttataagtt gttttctggg tcttcagact taggaactgg ttctgacaca atacttgcac
    31381 aaatagctgc aaaagcattg aatacatcta ttgatagaat ttccgtttat acaggagata
    31441 cagatatgtg tccatatgat actggtgcat acgcttcttg tactacttat gtaactggaa
    31501 atgctacaat aaaatctgct gaaagtttaa aacaaaagat attaaaagta gcacagaaga
    31561 aactcgattt accaatagat gatttaatac tatatgaaga cagagtttgt tatagtgaag
    31621 atgtagagaa gtttgttttg ttaagtgaat taggaaaaga atctgttggt ggtggaaatc
    31681 aagaagtttt atattcatct gaatcttatg gtatacaaga gagtgctaaa ccattcttag
    31741 caggatttgc tgaaatagag ttagataaaa caacagggga gtttaaagta ataaattatg
    31801 cttgttctac agattgtggt acagttatga atcctgcact tgctagaata caagttgaag
    31861 gtggtgtaac tcaaggtata gggcttgcaa tgtatgaaga ccctagacat ggttctgatg
    31921 gaaagcttca aacttctaat ctattacaat acaaagttcc aacaaaaaaa gatataggaa
    31981 acataattgt tgattttgta gataattatg agccaacagg tccatatgga gctaaatctt
    32041 taggagagat agttattcat acacctgcac cagcaatagc aaatgcaata tacaatgcca
    32101 caggagttcg tataagagaa cttcctataa cttctgagaa agtttatatg gccatgaaaa
    32161 aattaaatca gggaggtaaa taaatgattg ctattaaaag taaatactta atatcttctg
    32221 ctgatgaaat atatacaaat tctgctgttg tagtagaaaa tgacattata aaagatatat
    32281 tgccaaatga agaagttgaa ataaaatata aaaatattga agaaataata gataaatcag
    32341 atgctataat aatgccagga ttcgtcaatg gtcatatgca tcaatatgga gttctttcta
    32401 ggggaatacc ggcaaatgta cattttaccg actttgaagg tttcttaaat gactattggt
    32461 ggccatttat agaaaataga attggattga aagaagtgaa ggcaacaact aaagcatcag
    32521 caatagaact tatagaatct ggtgtggtag ctttttgtga taccctagaa gctccaaata
    32581 cagaagaagg aactttaatt gagcaaggaa aaatactaga agaaataggc atgaaagcga
    32641 ttttatcctt agaaagttgt gaaagaataa gctttgaaaa tggacttaga tgtttaaatg
    32701 aaaattctaa tttaataaag tggagtagag aaaatagtaa attaataaat ggaataatgt
    32761 gtacacatac aagcttcaca tgttctgata gatttattaa gaaagctaaa gaagatacaa
    32821 aaaaattaaa tgcaccaatg caatttcatt tatgtgagag tatatatgag ccaaactatg
    32881 ctgaaaaaca ttttggcaaa aaagcagttg actactataa tgatttagat atattggatg
    32941 aaactgtatt agcatctcag tgtgtaaaag taaatgatga agaaatagat atattaaaag
    33001 aaaaaggtgt aaaagtggtt catatgcctc ttagcaactg tgaagtaggt ggaggttttt
    33061 ctccagtacc aaaaatgatt aaaaaaggta taaaagtggc actgggtact gacggatata
    33121 taaatgattt ttttgaagtt atgagaggtg catttttaat gcataaatca gttgaagaag
    33181 atgcatcagt aatgccagca aatttagtat ttagaatggc aacagagcat ggtgcttatg
    33241 tattaggact tcaaaattct ggtaaaatag cagttggtaa taaggctgat ataatagtta
    33301 tggaagatga atttaaaact cctgtgactc ttgataatat atttgaccag atagtagttc
    33361 agggtaaaaa agaatttata tcaaatgtat acatagatgg tagacatatt ttaaaagaaa
    33421 aacagttagt tgatttagat aaaaaagcta ttgttaaaga aatgaaagaa gttgcttgtg
    33481 aattttggaa attttaacga atgtaaatat atatgaaaag gggaagcaga taatgtataa
    33541 ttttttaggt aaagaattaa aatcaccact tataataggt tcaggaccat taacatatag
    33601 tgcagctgga tgcaagatat taagtgatgc aggagcaggg gcagttgtga ctaagactat
    33661 aagaaaagaa agagctataa atccagcacc acatatggtt agaaatacag ctaatgctct
    33721 tttaaataat gaaaaatgga cagattttga gccagagcaa tggatagatt ttgaaatacc
    33781 acaaatgaaa agagatggga ctgtctgtat agcttcagta ggtcatacaa ttgaagaaag
    33841 tagcgaatta gtagagaaag ttgcaaatgc aggagctgac tttattgaat tagtatctta
    33901 tgactataga gatttgatac caatgcttaa agatgcaaag gaaagagtaa atatacctgt
    33961 aatagttaaa cttcctccta tgatagatga gataggcgat tttgctaaaa aattagaaga
    34021 agcaggagca gatgcaataa ctgcttgtga ttctgtagga ccagcattta gaatagacat
    34081 agaaacaggg caacctttat taggtggaaa tggaatagga tatttaagtg gagaaactat
    34141 caagccaata actttacaga gaatatatga aataaaaaaa caagttaata taccaataat
    34201 tggcttaggt ggatgtgtaa gtggagatga tgccttagaa atgattatgg caggtgctga
    34261 ctttgttggt atatgttcag tagtaatttt aaaaggcgca caagtaatat ctaaaataca
    34321 tgatgattta aaatctaatc ttaatagatt aggatataat actatagaaa atgcttgtgg
    34381 tatagtacat aatcatatgg gagatgttaa tgaaagactt agctttgagt ttacttatga
    34441 tgaagaaaaa tgtactaaat gtaagatgtg cgagagagtt tgtgcatatc aagcgagaaa
    34501 attgaatgat aagatggaat tgaatgaaga agagtgtaga tattgtggac tttgtatatc
    34561 agtttgtaaa ccaaaagcac ttggaagaaa aatttcatgt tcagagctga atgtatagat
    34621 tattttataa aaaacaaagt tttaaataaa aataagaaag gctatctcaa agagataagc
    34681 cttttttgtt gttgacctta tttttgttta tttgtagtta ttttagatat taattagttt
    34741 tgaagtaata aagtgaatca attttgttta attaaaattt taataattct gatatatatg
    34801 ttaacattgt atagcatttg tgtaaaatgt tataataatt aatagggatg gtgatgcaag
    34861 tgcaagagaa gattttagag ttattatata ctataaatga agatgtagtt agcataaaaa
    34921 atgatatgtc ggaagtaaaa caagaaataa atgtgttaaa agaagatatg tcggaagtaa
    34981 aacaagaaat gactgtaatg aaagaagata tgtcggaagt aaaacaggaa ataaatgtat
    35041 taaaaggaga tatgtcggaa gtaaaacaag aaatgactgt aatgaaagaa gatatgtcgg
    35101 aagtaaaaca agaaataact gtaatgaaag aagatatgtc cgaagtaaaa caggaaataa
    35161 atgtattaaa agtagatatg tccgaagtaa aacaggaaat gactgtaatg aaagaagata
    35221 tgtccgaggt aagacaagag ataaatataa tgaaagagga tatgtctgag gtaagacaag
    35281 agataaatac aatgaaagag gatatgtcgg aagtaagaca agaaatgact gtaatgaaag
    35341 aagatatgtc cgaagtaaga caagaaatga ctgtaatgaa agaagataca tcagaggtaa
    35401 aacaagagat aaatgtaatg aaaaaagaca tgtcagaagt gagacaagaa ataactgtaa
    35461 tgaaagaaga tgtttccgaa gttaaatcag aagtaaactt tatgcaaaat aaaataaata
    35521 acattaatga agatatgaat ggaattaagg acgaggtatc catagcaaat gaaaaattat
    35581 atggaattga aattaagatt gatagtctag aatgtgaaga taaatcaatg aaagagatac
    35641 agatagaaca gaataatatt ttaggaagtt tattacataa ttctgaaata aataaagcta
    35701 ctcatgataa tatagaacat aacatagcat atataaaagg tgacactaat tctataaaag
    35761 aggatatagc tgaaataaga agagacttaa atttagtaga gttagccact agtaaaaatt
    35821 ggagtgatat tgtcaaacta aaatcagtaa agtagtattt taattcacta attt
//
LOCUS       DAEPIN010000042        35536 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.042, whole genome shotgun sequence.
ACCESSION   DAEPIN010000042 DAEPIN010000000
VERSION     DAEPIN010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 35536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35536
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.042"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            80..781
                     /locus_tag="KR250_002206"
     CDS             80..781
                     /locus_tag="KR250_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4253 domain-containing protein"
                     /protein_id="HBH0068140.1"
                     /translation="MSIEDLYDFECVPLNNEIDIDVLFEKMIEDGKEKEYTPIIVIDE
                     KVGLLRKNIDMITKEYGSLENYREYCLTKYNEIDVNQFFDFRKSEISELLKELIDLED
                     DFYDYKHFRPIMNIDIFHEYNKVYIAKIPTIKPYEVFAYIPIGGFNDCPRDEEHIAIA
                     KYWYEKHGIFPIAIGCDTVQYSVKNLTKDGKKFDDLCIELVFYCEDIIIQGYETLKAL
                     KDVLKRSTIVLFWWD"
     gene            1051..1869
                     /gene="larE"
                     /locus_tag="KR250_002207"
     CDS             1051..1869
                     /gene="larE"
                     /locus_tag="KR250_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HBH0068141.1"
                     /translation="MEVNFVKEKEKLDKLKKMLLELGSVVVAYSGGVDSNFLLKVAKD
                     TLGENVIAVTIHAMMHSSREIEEAKQYTQNFGVKHIILNIENFDLKEFKENGVDRCYH
                     CKKYIFSKIKEVAKEHNIKYIVDGTNIDDLGDYRPGLKALSELGVISPLKDSSLKKEE
                     IRSLSKTLGLNTFNKPSFACLASRIPYGVEITDENLRIVEKSEEYLSNLGFSQFRVRM
                     HGDIARIEVGQEELGKFFENNNFNKVDTKLKIFGFKYVTLDMSGYKMGSMNLNV"
     gene            1924..3072
                     /locus_tag="KR250_002208"
     CDS             1924..3072
                     /locus_tag="KR250_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HBH0068142.1"
                     /translation="MKNYTLISPCFFGMEKMLAREITNLGYEIIKTEDGRITYKTDEF
                     GIAKSNMWLRCAERVHLKIAEFEAKSFDELFENTKRINWSRYIPYGAQFPISKASSIK
                     SKLYSTPDVQAIVKKAIVESLKKSYLEDGLLKEDKEKYPIFVFIHKDKVTISIDTTGD
                     ALHKRGYREKANKAPIRETLAAGLIYLTPWKAGRVLVDPMCGSGTILIEAAMIGINMA
                     PGLNREFISEKWRTLDKKIWWDVRKDAFNKIDNESKFKIYGYDIDEESIDIARENAEI
                     AGVDEYIEFNVGDATQFKSEDEFGFIITNPPYGERLEDKDSVKQLYKELGYAFRKLKN
                     WSYYLITSYEDFEYEFGQKADKKRKLYNGMLKTNFFQYPGPKPPRNNK"
     gene            3208..4596
                     /locus_tag="KR250_002209"
     CDS             3208..4596
                     /locus_tag="KR250_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBH0068143.1"
                     /translation="MKVLKLITVVALMMLFVTGCNIKTESPEQLAKIPDYDDTKKVLY
                     DGIDQLLKPDSSMILPSNTKEVGKINKVDLNSDGIDELVVFEQKEDLSNNVSQVGFVT
                     LSYNGEKYVLGDHFLENGESIEYANFYDLDSDGYKEVILLVKSKDKTNMHIYKVVDNE
                     ITKIYDLDASWLQNKEDFNDMKVKIGYIDGDDKLDILMLHLNNKTNEMFASVANFDGK
                     MKIKDSVKFENVKNLSELYITLGNVASSVGNSSAAIKGIVLDIPILKDNNYITQILYM
                     KDGKINKAFSDYDKTITKSYYIPVDDIDKDKIIEIPIVAGSTGNNKNTYSSKSSATIS
                     WYRWNGKEGADSSLIFTSQIYYNYKYNFKLFIPNNLFDKILVEQEFVGEKAVFKFYYF
                     DYSDRKNLFNIVVESKNKLEDRKNTGTQNSIVLQESDEYTFLLVVNNANEMDKLDMTI
                     DALKEYFSLIYG"
     gene            4625..5323
                     /locus_tag="KR250_002210"
     CDS             4625..5323
                     /locus_tag="KR250_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0068144.1"
                     /translation="MKEKILILEDEIGIRSFVSINLKREGYEIVEAGTGREAIEKMTT
                     EKDITIALLDVMLPDISGIEVCKFIRENFDQVGIIMLTAKAQEDDKIEGFISGADDYI
                     IKPFSIKELLVRVSALLRRVAKDDSSVKSSEIVSPPFILDIDKRKLFKNGKEIELTPT
                     EFSIVKYLISNAKQSLSRDQILDEVWGTNYLYDFKIVDVNIRRIRNKIEDDPSKPKYI
                     QTIWGYGYCFRKEE"
     gene            5326..6759
                     /locus_tag="KR250_002211"
     CDS             5326..6759
                     /locus_tag="KR250_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBH0068145.1"
                     /translation="MLHFEGLRKKVIKNYFIIIIIMVTLFEGLFMFYIQNYYYDSVKQ
                     LLESEIKYADEYNAITMETTSFEKKVKNIFDKQPLTKNSEFGISIIDKDKNIILDQYG
                     FKSKEKANYEDVNNALKDIKTKNLTPYTYRIPDTGEHVMSISLPLKVNNIIEGVVRYT
                     VSLDAIDNAILKQATWLILAGIFILIIAILISLKFAETLIKPLRELKKFANELAVGNY
                     NIKLEKMKIVDDEIGDLAQTFEHMAHEIDKSEKLKEEFISSVSHELRTPLTSIKGWSE
                     TLGYESITREELDLGLGIIQDETERLIKLVEELLDFSRLSSDRIKLHVDIVDVEGLIV
                     GVVNQLKVKAAEKDISLLFEFENEFIENIQGDKNRLRQVLINLIQNSFKFTSQGGYIK
                     VVASQDEEITTISVEDNGSGIEKQNLNKVLDKFFQEDYNKAGSGLGLAISNEIVKLHG
                     GRMKIESEKNVGTKITFNIKNKFAKQA"
     gene            6781..7518
                     /gene="truA"
                     /locus_tag="KR250_002212"
     CDS             6781..7518
                     /gene="truA"
                     /locus_tag="KR250_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HBH0068146.1"
                     /translation="MRNIKMIVAYDGSRYKGYQKLGDNNMTIQEKLENVLSKMTNETV
                     EIIGSGRTDMGAHARGQVVNFRTNCMDSLDKIQKYLYEYLPEDIVVKTVEEVDERFHS
                     RYNVKSKTYMYKIDNNKYHNPFIRKYATHVSKKLDLDRMRKASEYLVGEHDFTSFASS
                     KSKKKSNVREIYSINIKEDDNVIEIYVEGNGFLYNMVRIIVGALIDVGLKRRAPQDIK
                     YMLESKDRCQSSDTAPAKGLCLWKVTY"
     misc_binding    7629..7836
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            8089..9288
                     /locus_tag="KR250_002213"
     CDS             8089..9288
                     /locus_tag="KR250_002213"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HBH0068147.1"
                     /translation="MKEKVVLAYSGGLDTSIIIPWLKENYEDIDVIAVCGNVGQEDKM
                     EDVYEKALQSGASKAYVDDISEEFVTETIFKAVKAEAKYEGKYLLGTSLARPIIAKKL
                     VEVAHKEGAKYICHGCTGKGNDQVRFEATIAALDPTIKVIAPWRIWDIKSREDAIDYA
                     EKHNIKVTATKAKIYSVDANLWHVSTEGGDIEHLENEHKKDVYKQCVDPEDACDVAEY
                     VEVYFEKGVPKKVNGEELSPVALIHKLNELGCKHGIGVIDIVENRLVGMKSRGIYETP
                     GGTILYEAHNILESATLDKDTLHFKQMVSYKYGELIYNGLWYCKLRESIDAFMEQTQD
                     NVTGTVKVKLYKGNIKPAGIFTENALYDEGISSFGNSELYDHKDAEGFINLFTLPLKI
                     RAMKAGK"
     gene            9535..10581
                     /locus_tag="KR250_002214"
     CDS             9535..10581
                     /locus_tag="KR250_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4872 domain-containing protein"
                     /protein_id="HBH0068148.1"
                     /translation="MRKIIEIKHHSHDYECMWNGIEDLYINKTGEYLPNDFFFLLSGF
                     GSFCYMKTNKADLKRMVALGDGRTKQMYKFLAPIVGFEYKHHEFKKYEQALKKAKSEI
                     DDGYPVILGALDMYYLPYYKKLYHKEHIPFHYILMVGYDDEGIYLYDCGRTELLHLSY
                     DKLRESMNCNSPGLSKENTICTIRMNTSKNKYNIAREAIAIKRDMFLNPPKGFLGYKG
                     FEKFIKELPQWNTELDKDDYDKILTNMVMFFGTVPTIPNAIKGIKEPDTVQFKGGFDK
                     MSRMLNSIGEEYKNNSFIQVADIFDKTAIIIEKISNIIIDYLTQTCDDTEQLPLLFSE
                     VLEHMKTGYLILDV"
     gene            10689..11852
                     /locus_tag="KR250_002215"
     CDS             10689..11852
                     /locus_tag="KR250_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBH0068149.1"
                     /translation="MRTVKQVSDLTGISVRALHYYDEIGLLKPNKITDAGYRLYDDES
                     IKTLQQILFFKEIDIPLREVKEIMSSQYFDKVEALKNQKKLLILKRKRLDELIELINQ
                     TLRGEGNIDFKEFDMSEYFNVLEEFKREHRNKVIKIYGSVEKYNEYIERVKSNEEKIA
                     KMAIKQYGTIEKFAKAIKTNFSSDILNLGEKFDRYKNDCLKEKHPKLKELYRKLVEDL
                     SRNPSSTDLQEIAKAITDISKKDYEIFSMDTGDDNWYYMVQNYLVNPMWIEEVDKKYG
                     SGAGKFIGQVLKTYLRDRKPKINTLYEKLVEDLSRDCSSREVQSIVEEIDNEMKRSNE
                     FYKIDNGERYFDYMSELYLQDSNYIKVTDKNYGDGASKFIGEAFKIYFDNNNC"
     gene            complement(11941..12321)
                     /locus_tag="KR250_002216"
     CDS             complement(11941..12321)
                     /locus_tag="KR250_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4363 family protein"
                     /protein_id="HBH0068150.1"
                     /translation="MKSTTFVILWTILFVLFGFYVNNKLYDFTEGYKDNISVLEKSIE
                     NEDWEKAQKEADSISTSWNKEKNHWYKVLNHEYFDEIGLRFNILDKAIYTENKLKSLE
                     EVESIKTYLGNIIESVKFDVNYIF"
     gene            complement(12324..12983)
                     /locus_tag="KR250_002217"
     CDS             complement(12324..12983)
                     /locus_tag="KR250_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HBH0068151.1"
                     /translation="MTVVLIRSIILYITVLIALRVMGKGEIAEMNCFDLVITLLIAEV
                     ASVPMENNNIPIINGVAAISGLVIMQTLISFLSLKSRKLSSFLSGKPSVLIDKGKIVY
                     KELKKERISIDELLEQLRIQGYFNLKDVQYAILETDGNLSVVPASSYNSTPPRAFNHL
                     PIPLILDGRIINKNLDIAQKDTNWLMGILKSNHIETFKDVLICVLDENDKIFIQNKKG
                     D"
     gene            complement(13089..14063)
                     /locus_tag="KR250_002218"
     CDS             complement(13089..14063)
                     /locus_tag="KR250_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HBH0068152.1"
                     /translation="MKYKLFIDFEFNILDDNKNKPKEYNGAELISIGGVLVDNEFNTI
                     DNYYSLVKPKYNKILSNKCKNLTKLNQLDINNAPNLLYVMDDFYKWFCKFDDVTIYNW
                     GDFDITGLLTSFRVYKYTGKCLELFNMMIDIQPFISQHITYNNRILSKQISLLNMKKI
                     FSVEGDIKHNSLSDAVDLMNVCKCFFFNSPKNIDILEELYNKLPALKTPLYYIPYFED
                     ENFELKFDRTPEDIIIYLKQILTILNISKKEIYFKKRSVLVNNSKIINFKNLSSSFKL
                     IKLNEFEEYPDFILKLGDKKSFVESVIKINKSNRKSIKNLILKLSKKP"
     gene            complement(14103..14450)
                     /locus_tag="KR250_002219"
     CDS             complement(14103..14450)
                     /locus_tag="KR250_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0068153.1"
                     /translation="MLNLKEIREEIIKKYIGDSLISELTEELKNILITDLQSNPFKSN
                     IELSIIPANIEIFKKIENILIDSKVNLKMGWERILINYILKDLFELNYEINKSCNEPL
                     MFRVIISIDSYDA"
     gene            complement(14668..15624)
                     /locus_tag="KR250_002220"
     CDS             complement(14668..15624)
                     /locus_tag="KR250_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HBH0068154.1"
                     /translation="MKKILITGALGQIGSELTIKLRNEYGEQNVIASSRRVKEGNPVC
                     ESGIFEILDVTDKNRFFEIAKKYDVDTIIHLASLLSAVAESKPLEAWNLNMNGLINGL
                     EIAKELDCKFFTPSSIAAFGENSPKNMTPQDTLQRPNTMYGVTKVSGELLCDYYHSKF
                     GVDTRGVRFPGLISYVTPPGGGTTDYAVDIYYEALKNKRYKSYIAEGTKMDMMYMPDA
                     LQSIVDLIEAPADKLIHRNAFNITAMSFSPEEIADSIKKYIPDFVIEYDVDPVRQSIA
                     DSWPNSLDSSSAVKEWNFKFSYDLDKMTKDMLEKLSEKGIGK"
     gene            16067..16435
                     /locus_tag="KR250_002221"
     CDS             16067..16435
                     /locus_tag="KR250_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HBH0068155.1"
                     /translation="MTRPSIYHSQGYTCAEALIKSYNEEHNTDIPISIGSGMGVGMNV
                     GSVCGAVNAAVVIIGYLNGRNSNLDENIARKYSRELLNRVRNKYSSEICVSLKKNKVT
                     CEEIINFTYDALNDMLKNQK"
     gene            16860..17105
                     /locus_tag="KR250_002222"
     CDS             16860..17105
                     /locus_tag="KR250_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0068156.1"
                     /translation="MFIDEELEGYILTCKISEDFKNIPEYSDEEFYVTVYKDESSDSG
                     YYALLENKEERVVWDGEVVANNIFNNLWIVINKVKTG"
     gene            17204..17467
                     /locus_tag="KR250_002223"
     CDS             17204..17467
                     /locus_tag="KR250_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1883 domain-containing protein"
                     /protein_id="HBH0068157.1"
                     /translation="MEYSYSKMNLKKGDIVEVNLEKQANVILLDHINYVKFKNQRNYD
                     YYGGFAKKNPCRMRVPNTGTWYLVVNQDGNSGIVNFSINTIQN"
     gene            complement(17752..18699)
                     /locus_tag="KR250_002224"
     CDS             complement(17752..18699)
                     /locus_tag="KR250_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013378669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HBH0068158.1"
                     /translation="MSNVTAEQKKEVKGRGFLPSRDGEHFAARIITVNGVINASQAKK
                     IAEAAEKFGNGQLAFTTRLTVELPGVKFEDIEALSEFIASENLITGGTGSRVRPVVAC
                     KGTVCVHGLADTQALAAEIHEEFYKNWYDVKLPHKFKIGVGGCPNNCIKPDLNDLGIV
                     GQRVPDYDSELCVGCKKCAVVEVCPVKAAKLTDKGKLEIDSNLCNNCGKCIESCNFDS
                     IEEKESGYKVYIGGKWGKSVRPGTQIDRLFSKEELMTLIEKAILLFREQGNTGERFGI
                     TIDRIGADKFIEMLLSDEVLERKEEILEALLHLTGGAVC"
     gene            complement(18702..20333)
                     /locus_tag="KR250_002225"
     CDS             complement(18702..20333)
                     /locus_tag="KR250_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBH0068159.1"
                     /translation="MKVLIIGGVAAGTKTAAKIKREMGDNCSVTILNKGDDISYAGCG
                     LPYYVGKIIEDKSSLIVNTPESFSKLTGAEVRCGVEVTSIDRNAKQVTLKTLSTGEKE
                     NLSYDKLVIATGADPIKPPIPGIDLPGVFFVRTPNDAIKLRDSIENGIKRAVVIGGGF
                     IGLEIAENLSAMGIRVSVVDMAEHVMPGFDTDFAEYVENHMADKGIMIFTGDQVVGIE
                     GENKVEKLRTKNRAIKTDLVVMSVGIRPNTGFLKDTGLEFAPNNTIIADEYMLTNDKD
                     IYVVGDCAFVKNSLTNKPTWSPMGSSANHEGRICAQNISGKSKTYNGVLGTTVVKLPE
                     LNAGKTGLSKEIAEKEGYNVCSVTIATDDKAHYYPGASNFIIRMVAEKNTRKLLGVQV
                     MGPGAVDKIVDIAATAITLGADLYSLESMDLAYAPPFSTAIHPFVVAVNVLQNKLNGE
                     LDSVLLDEIEDFDSWTKLDVSKAPSIPSLRYIPVGEINGEIEGLAKDEKILLICAKGK
                     NSYMAQNRLRRFGYTNTKVLEGGILFYPNLKVMEE"
     gene            20463..21380
                     /locus_tag="KR250_002226"
     CDS             20463..21380
                     /locus_tag="KR250_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBH0068160.1"
                     /translation="MTIRSLEIFVKVAECGKMSEVARNMYITQSSVSQAISEIEKEYG
                     VKLFDRISKKLYLTEAGKKLLGYGRHLLAVNEEMNDCMKHCAKNIRIRVGATVTVGTC
                     VISPIMLELYKVNPLIEPEVFVEDTRLIAKKLLNSELDIAIVEGKIKHPDIVTKSIIN
                     DNLVLICSHKHEFYKRDSIKVSELSNQPLIMRELGSGTRAQLEKQLKELKIPMNIRWS
                     CYNSEAILRAVVDNFGIAVISELLIEDYLKKHLLWACDIEGINLHRTFDIAYHRSKFF
                     TENISAFFDISVEYGKKQARKKSRSVNSL"
     gene            complement(21437..22018)
                     /locus_tag="KR250_002227"
     CDS             complement(21437..22018)
                     /locus_tag="KR250_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBH0068161.1"
                     /translation="MGITLAKGQKVSLTKSNPGLKKVIVGLGWDINKYDGGFDFDLDA
                     SAFLTGTDGKVTNDGDFIFYNNLKHASGAVEYMGDNRTGVGDGDDEQINVDLSKIPQN
                     IAKISFSVTINEAITRRQNFGQVENSYIRIYNEETNEELIKYELGEDFSIETAIVVAE
                     LYRHNGEWKFNALGSGFEDGLAGLCKNFGVNIG"
     gene            complement(22058..22624)
                     /locus_tag="KR250_002228"
     CDS             complement(22058..22624)
                     /locus_tag="KR250_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBH0068162.1"
                     /translation="MSVSLQKGQRVSLVKDNNPVKNLVVGLGWDMNKLGKKNYDLDAF
                     AIALTNQDKMNVRGDLVYFGNLKHPSKAIIHTGDNLTGKGEGDDEQIIVNLEDIPEYV
                     HKIVFGVSIYKAKKRDQDFGQMNNSFIRLIDSNSKQELFKYNLQEDFSGKVTVLAGEI
                     YRRNEEWKFNALGIGQNEELRELINTYK"
     gene            complement(23080..23940)
                     /locus_tag="KR250_002229"
     CDS             complement(23080..23940)
                     /locus_tag="KR250_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBH0068163.1"
                     /translation="MLLKKDRITFSEILILIVGCILMAISLNLFFNPHAIAAGGITGL
                     GVVLNSLFGVELWIVNLLLNVPLFIFAYKILSKKDCFKTVLGIIFLTIALKLTANMAT
                     LDITNDMYLAIISGSILMGVGQGLIFRINGSTGGTDLMALLLNKYFPTFSIPVLMGIV
                     DCVVVVLSGIVNRQVEIALYSTVALYILVKVSDLLIEGFNYSKSFTIISDLSKDISKK
                     IMEDLDRGATILKGEGAYTGENKNVLLVVVEKKEVVELKKLVKNVDPNAFIIITDIHE
                     ALGNGFKKIE"
     gene            24089..24979
                     /locus_tag="KR250_002230"
     CDS             24089..24979
                     /locus_tag="KR250_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBH0068164.1"
                     /translation="MAKLTLEHIKGNTYYIPLPTIVGVYVDGKDAILIDSGNNKDTAR
                     QVLRLLEEHNLNPKLIINTHSNADHIGGNAYLKNQTKCKIATTKIEGYFTENPILESA
                     FLYGGYPSKALKNKFLLAKESEVDYIIPSNGKIVDTELEAISLPGHYFEMIGVRTPDN
                     VLFVADSLTPENIITKYHFFFLLDIDSQFKTLEKLRMLEADFFVPSHSVKTTDIKSLI
                     NINKKKMEEIIDNIKKVCYEPVMIDEVIEKMCDLYNVKLDANQYVLVGSTIRSYITYL
                     YENNMVEYIFDGGKMMIKAA"
     gene            25197..26951
                     /locus_tag="KR250_002231"
     CDS             25197..26951
                     /locus_tag="KR250_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp23"
                     /protein_id="HBH0068165.1"
                     /translation="MYIMNKKFKKFSKKLSILILVLTMFLFNNLTANAQIKEDSIIGI
                     DRYETAGLIADRQTYDTVILVNGDKNLSDGLSSSGLAGAINAPILLTKKSEIPKATLN
                     RLDNKSLNVVKKVYIIGGYNTIENSVEKDIKRKGIQVERINGNNRIETSYNVAKKINE
                     VSSVKEVMLTNGFVGEADAMSIAPVVAKNKGAIILTDGKSVPFKTEGLNVYAIGGKSA
                     INEDLVNETKAIRIGGNDRFETNKKVMEKFYNGATDFYITNGYQLVDALTLSPLAKEK
                     PIVLVADGSNKGILKGAKSITKVGGIDANIYKQCLDMIKYNNMDMTPNILKHLTSVEG
                     NEVSEILIESDNLGVVVEKTNTNKFEFDYVSVTNEKNCTFSVYKENSSNNIKNGKLVV
                     SAKKKIDKKDSDLQDMNGDNMINANKDRMVNVIKIGVPDKMYSNFKVDVESGSVELYN
                     IKGGATVDVNDGIANIVDNNVTNPFNINTNSGISGVTAETISSEIKFRANNGIVNVTA
                     TNINGNISLFGSDNKDTFDGIFKLNLKKEPSNLHLKLIGNGINKLPEGWSKDYILGNG
                     QPIIEVKNNGINNISIGE"
     gene            27335..28321
                     /locus_tag="KR250_002232"
     CDS             27335..28321
                     /locus_tag="KR250_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBH0068166.1"
                     /translation="MKKITINDIANLAGVSKSTVSRYLNNKDISDSTKEKIKTIIDEY
                     GYEPNAFAQSLRAKKTYFIGIITPCLDSFVKSKIMMAIDEELKKLKYTSLIINTNRKI
                     RSEIDSISKLASLKVDGIILIGTEITKEHKNEIEKLDIPIVVVGQKVDGINSIVNDDY
                     GAGYKMGQYIANKGYKNIVYLGVDESDISVGLNRKNGVLNGLKDKGYDAKVFYTDFDQ
                     EMSIQRSGEMLESENPDIIICATDNIAIATMKEINKRGKSIPQDISVAGFGGYDVLSI
                     ISPKLTTIKFENKNAGKVAANTIVNLIQERKEPLLKEIKFELIEGESTINKN"
     gene            28490..28638
                     /locus_tag="KR250_002233"
                     /pseudo
     CDS             28490..28638
                     /locus_tag="KR250_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423822.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIB"
     gene            28657..30138
                     /locus_tag="KR250_002234"
     CDS             28657..30138
                     /locus_tag="KR250_002234"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HBH0068167.1"
                     /translation="MYKEPKYRTILESTKEELNKLRNISLNDKYKPLFHIHPQHGLLN
                     DPNGLAYYNGKYHVFYQWYPYDATHGMKHWAYVSSDDFVNWNREDVALIPIESYESHG
                     AYSGNAIEVDGKLHMYYTGNIKYSAEDRYAYQNLAIMNKDGKITKYENNPIVSEIPKG
                     YTGHVRDPKVFKRKDKYFMLLGAQTSDKKGVIIVYESKNSIDWNFKGELNVKNIDEDF
                     GYMWECPDYINIDEKDILIFSPQGVEPKGFDYQNIYNVVYAIGNMDLDNLTFEIDTMK
                     ELEKGFDFYAPQTFIKDSQIILFAWAGMGEVLYPTDKNKWAHCLTVPRKLNIKNNKLL
                     QMPVDELIKLRYDETSGQNTIKNNINIIENDENVYELNINIKNIDSNKFGLELFSSQD
                     EGVKLEFNKLGNIVTLDRSNFKKVFGVEYGTNRKEYINIDENTNIKVLADRSILEIFI
                     NEGEVVFTSRIFAKENSNQIRVYSDKIVEYEYTKFKLKQGIEL"
     gene            30164..31135
                     /locus_tag="KR250_002235"
     CDS             30164..31135
                     /locus_tag="KR250_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HBH0068168.1"
                     /translation="MKKVISIGEALIDFIPNQNGGKLKNVSEFRRVAGGAPANVSAVV
                     AKLGGKSSFISKLGKDAFGDYIIDVLNEVNVNTDYVLRTSKANTGLAFVSLKEDGNRD
                     FSFYRNPSADMLLEADEVKKEWFNNCHILHFCSVDLIDSPMKLAHKKAIEYALESNSI
                     ISFDPNIRLPLWDSEQSCKKAISEFLPFAHIVKISDEELEFITGENDIEKSLHKLFVG
                     NVELVLYTKGKDGVDAYTKKVKGMCKGVKVNAIDTTGAGDSYIGSFLYTLLYKQITLN
                     DIKEMKQETLNEYLEFSNYYAARSTTIKGAISSYATKEEITEFIKNL"
     gene            complement(31391..31993)
                     /locus_tag="KR250_002236"
     CDS             complement(31391..31993)
                     /locus_tag="KR250_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBH0068169.1"
                     /translation="MEVCILMGSPKKKGNTAAILKPFIEELENYNSNIEVIWLYDCKI
                     EPCIACKKCQDNLFDFGCYCKDDVQKIFKKILMCDLIVFATPIYSWYCTSPMKALLDR
                     LVYGMNKYYGNEKGPALWAGKSVALITTCGYRPEKGADIWENGMKRYCKHSQLKYIGM
                     LVERDLGKPNFMNKEKEEHSRQFAKQIYYILSNSIELDNC"
     gene            32274..33485
                     /locus_tag="KR250_002237"
     CDS             32274..33485
                     /locus_tag="KR250_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HBH0068170.1"
                     /translation="MYSNEDYKFIEENAPCYIYDETNIIEKCKELKNNILGFDFLYSI
                     KTNPYNKIVKSIAEQGFGADASSTNEVMISSKCGVSKDDIYYSSPGKTEIDIETVLDK
                     CIIIADSITEIELINKISKKHNQKVKIGIRINPDFTMDDDSAKSSKFGIDIEDMSSVF
                     KMLSMCDYVSVVGIHIHIKSQVLDFEKLGHYYEKCFFIALNLNKSKYINIEFINFGSG
                     LGVAYDNLRENPLNLKELSKLTQKIAFTNSNTLKSRLLIESGRFIVCKSGNYYVKIVD
                     IKVSRGKKYLIVKNGLNGFMRPIISNLIENITCGVELEGQEPLYTSNHAFSLSVLNNL
                     KEEEYVNVVGNLCTSLDVIANNVKLKKANIGDLISISNAGSYSYSLSPLLFSSYEFPK
                     QYYKRKDGYIE"
     gene            33553..33750
                     /locus_tag="KR250_002238"
     CDS             33553..33750
                     /locus_tag="KR250_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HBH0068171.1"
                     /translation="MELWKRNLFVCWIGMFFSSIGMSQIAPILPLYIKQLGVTDVSLI
                     QQYSGIIFGCTFVVAAFFSPI"
     gene            33784..34755
                     /locus_tag="KR250_002239"
     CDS             33784..34755
                     /locus_tag="KR250_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBH0068172.1"
                     /translation="MLLRASIGMGIVIFTMAFVQNVYQLLGLRILQGVFTGYATACTT
                     LIATQTDKNHSGWALGTLATASTTGSLIGPTVGGYIESILGLKSTFIITGGLLFVSFI
                     ISILFVVDNFKPKEIKESISIPKEQLNILPNKFLIASIFVTTFITQLALYSIEPIVTI
                     YISQLTNFASNVALIAGLTFSASGLANLLAAQKLGKMSDKIGPQKVLLISLLWAGIIF
                     IPQAFVKTAWQLMLLRFLLGLSVAGLNPSVNSLLKKIAPEEYVGKIFGYNASAQYIGC
                     SSGAFLGGQISAHLGIRTVFFSTSLLLFRNALWMYKFTGLFIKDKTK"
     gene            34942..35319
                     /locus_tag="KR250_002240"
     CDS             34942..35319
                     /locus_tag="KR250_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBH0068173.1"
                     /translation="MTISKIPQAELKVMKFIWEKNDTVTSKETIEAMERKYGWKQTTT
                     LTLLSRLVKKHFLSAEKIDRYTHYTIVVGHKEYLSVETKDFLSNIHDNSLQSLISALH
                     DDEVVDKDMLDLFESHFKNLEEE"
BASE COUNT        13873 a         4063 c         5553 g        12047 t
ORIGIN      
        1 aaataatagt ggtattaaaa atctgacaac aaaaataaaa ctttcaaaaa attaaatcta
       61 aaaagaatag gagataaata tgagtattga agatttatat gattttgaat gtgttccttt
      121 gaacaatgag attgatattg atgttttatt tgaaaagatg atagaagatg gaaaagaaaa
      181 agaatataca ccaattatag tcattgatga aaaagtaggg ctattaagaa aaaatataga
      241 tatgattact aaagagtatg gttctcttga aaactataga gagtattgtt taacaaaata
      301 taatgaaatt gatgtaaatc aattttttga ttttaggaag agtgagattt cagaattatt
      361 aaaggagcta attgatttag aagatgattt ttatgattat aaacatttta gacccattat
      421 gaatatagat atatttcatg aatataacaa agtttacata gctaaaattc caacaattaa
      481 accatatgaa gtatttgcat acatacctat tggcggattt aatgattgtc ctagagatga
      541 agaacatata gctattgcaa aatattggta tgaaaagcat ggaatctttc caatcgcaat
      601 tggatgtgat acagttcaat attctgtaaa aaatttaact aaagatggta aaaaatttga
      661 tgacttatgt attgaattag tattttattg tgaggatatt attatacaag ggtatgaaac
      721 cttaaaggct cttaaggatg tattaaaacg ctctacaata gtgctttttt ggtgggattg
      781 atattctatc atttagatag tattaaattg ttagtcacca agaatccact tgctttagtt
      841 atagggagtg tgtcaaacta ggtttttata cacagtgtag catagtaata tgtttaaatt
      901 acaaaggtta aaaaaatact ttaatatttt taatattata attaatagat ttaaggtaat
      961 tataatttat aaaataaata ctttatatgt tataatcatt aagaatgtaa aaaaataata
     1021 tatagtttta tagttcatat ttttccagat atggaggtaa attttgtgaa ggaaaaagaa
     1081 aaattagata aattaaaaaa aatgttactt gaattaggca gtgttgtagt tgcttattct
     1141 ggtggagtag atagtaattt tttgcttaag gtcgcaaaag atacattagg tgaaaatgtt
     1201 atagctgtga ctattcatgc aatgatgcat tcaagtaggg aaattgaaga ggctaaacaa
     1261 tatactcaaa attttggtgt taaacatatc atattaaata tagaaaactt tgatttaaaa
     1321 gaatttaaag aaaatggtgt tgatagatgt tatcattgta aaaaatatat attctctaaa
     1381 ataaaagagg tagcaaaaga acataatata aagtacatag tagatggaac aaatatagat
     1441 gatttaggag actatagacc tgggcttaag gcattgagtg aattaggagt aattagtcca
     1501 ttaaaagata gtagtttaaa aaaagaagaa attaggtcgc tttcaaaaac attaggatta
     1561 aatactttta ataaaccatc atttgcatgt cttgcgagta ggatacctta tggagttgaa
     1621 ataacagatg aaaatttaag aatagtagaa aagagtgaag aatatctatc caatttaggt
     1681 ttttctcaat ttagagtgag aatgcatgga gatatagcta gaatagaagt tggacaggaa
     1741 gagctaggta aattttttga aaataacaat tttaataaag ttgatactaa attaaaaata
     1801 tttggcttta aatatgttac actagatatg tcaggatata aaatgggaag catgaattta
     1861 aatgtgtaga ttattttaaa aataaataag ttttatagtt aatataagga aacaggaggt
     1921 tttatgaaaa attatacgtt aatatcgcca tgtttttttg gaatggaaaa aatgctggct
     1981 agagagatta ctaatttagg atatgaaata ataaaaacag aagatggaag aataacatat
     2041 aaaactgatg agtttggaat agcaaagtca aacatgtggt tgagatgtgc tgaaagagta
     2101 catcttaaaa ttgctgaatt tgaggcaaaa agctttgatg aattatttga aaatacaaaa
     2161 agaataaact ggtctagata tataccttat ggagctcaat ttcctatatc aaaagcttcc
     2221 tctataaaat caaaattata cagtacacca gatgtacaag ccatagtaaa aaaggctata
     2281 gtagaaagct taaagaaaag ttatttggag gatggtttgc ttaaagaaga taaggagaaa
     2341 taccctattt ttgtatttat acacaaagat aaagtaacca tttctataga tacaacagga
     2401 gatgctttgc ataagagagg atatagagaa aaggcaaata aagctccaat aagagagact
     2461 ttagctgctg ggcttattta tttaactccc tggaaagcag gtagagtttt agtagaccct
     2521 atgtgtggtt ctggtactat attgattgaa gctgctatga ttggtataaa tatggcacct
     2581 ggcttaaata gagagtttat atcagaaaag tggagaactc ttgataagaa aatatggtgg
     2641 gatgtaagaa aagatgcttt taataagata gataatgaat ctaaatttaa gatttatgga
     2701 tatgatatag atgaagaatc tatagatata gctagagaaa atgctgaaat agcaggtgta
     2761 gatgaatata tagagtttaa tgtaggagat gcaactcaat ttaaaagtga agatgagttt
     2821 ggatttataa tcactaatcc tccttacgga gaaagattag aagataaaga cagtgttaaa
     2881 cagttgtata aggagcttgg ttatgcattt agaaaattaa aaaattggtc gtattatttg
     2941 ataacttctt atgaagactt tgaatatgag tttgggcaaa aagcagataa aaaaagaaaa
     3001 ttgtataatg gtatgttaaa aactaacttt ttccaatatc cgggtcctaa gcctcctaga
     3061 aataataaat aagttttaca tgatttattt gtaaaatata aaattttatt aaaataagta
     3121 aaacattatg atgccctgaa acgtctaatt attgtgagga gatttttgtg aacaaattta
     3181 aagatttata ggattaggag gagttaaatg aaagtgttaa agcttattac tgtagtagcc
     3241 ctgatgatgt tatttgttac tggatgtaat ataaaaactg aatctccaga acaattagcg
     3301 aagatacctg actatgatga tacaaaaaaa gtattgtatg atggtattga ccagttacta
     3361 aaaccagatt catctatgat attgccttct aatacaaaag aagtaggaaa aataaataag
     3421 gtagatttaa acagcgatgg aattgatgaa ttagtagtat ttgaacaaaa agaagattta
     3481 agtaataatg tgagtcaggt agggtttgta accttgagtt acaatggaga aaaatacgta
     3541 cttggagacc attttttaga aaatggcgaa tcaatagaat atgctaattt ttatgatttg
     3601 gactcagatg gatataagga agttatctta ttggttaaat ccaaagacaa aacgaatatg
     3661 catatatata aagtagtaga taatgaaata acaaaaatat atgacttgga tgcatcatgg
     3721 cttcaaaata aagaagattt taatgacatg aaagtaaaaa ttggatatat agatggagat
     3781 gacaagttag acatattgat gcttcatttg aataataaga ctaatgaaat gtttgcaagt
     3841 gttgcaaatt ttgatgggaa aatgaaaatc aaagattctg ttaagtttga aaatgtaaaa
     3901 aacttaagtg aattatatat aacattaggt aatgttgctt catctgttgg aaattcttct
     3961 gcagctataa aaggtattgt acttgatatt cctatattga aagataataa ctatattacg
     4021 cagatacttt atatgaaaga tgggaaaatt aacaaagctt ttagtgatta tgataaaact
     4081 attactaaat cttattatat acctgtagat gatattgaca aagataagat tatagaaata
     4141 cctatagttg caggcagtac aggtaataat aaaaatactt atagttctaa atcatctgca
     4201 actataagtt ggtatagatg gaatggaaaa gaaggtgctg actcaagttt aatatttaca
     4261 agtcagatat attataatta taaatataat tttaaattat ttattccaaa taatttattt
     4321 gataaaatac ttgttgaaca ggaatttgtt ggagaaaaag cagtatttaa attttactat
     4381 tttgactatt cagaccgtaa gaatttattt aatatagtag tagagagtaa aaataaactt
     4441 gaagatagaa aaaatacagg tactcaaaat tctattgtac ttcaagagag cgatgaatat
     4501 actttcttac tagttgtaaa caatgcaaat gaaatggata agctagatat gactatagac
     4561 gcattaaaag aatatttctc attaatatat ggttaatatt atactcaatt ggaggtttta
     4621 ttttatgaag gaaaagatac ttatactaga agatgaaatt ggtattagaa gttttgttag
     4681 tataaactta aaaagggaag gctatgaaat agttgaggca ggtactggaa gagaagctat
     4741 agaaaaaatg acaacagaaa aggatattac aatagctctt ttagatgtaa tgcttccaga
     4801 tataagtggt atagaagtat gtaaatttat aagggagaat tttgaccaag taggtataat
     4861 aatgcttact gctaaagctc aagaagatga taaaatagaa ggatttatct caggagcaga
     4921 tgattacatc ataaaaccat ttagtataaa agagctttta gtcagagttt ctgctcttct
     4981 tagaagagta gcaaaggatg attcaagtgt taagtcgagt gagatagttt ctccaccatt
     5041 tatacttgat atagacaaaa gaaaattatt taaaaatgga aaggaaattg agcttacacc
     5101 aactgaattt tcaatagtga aatatctaat atcaaatgct aagcagtcgt taagtagaga
     5161 ccagatatta gacgaagtat ggggaactaa ctatttatat gattttaaaa tagtagatgt
     5221 aaatataaga aggataagaa ataaaataga agatgaccca tcaaaaccaa aatatataca
     5281 aactatttgg ggttatggat actgttttag aaaggaagaa taatagtgtt acattttgaa
     5341 ggtctgagaa aaaaagtaat aaaaaactat tttataataa ttattataat ggttactctg
     5401 tttgaaggat tgttcatgtt ttacatacaa aactactatt atgattctgt caaacagcta
     5461 cttgaatctg aaataaaata tgctgatgaa tataatgcta ttacaatgga aactacaagt
     5521 tttgagaaaa aagtaaaaaa tatatttgat aaacaaccct taactaaaaa ttcagaattt
     5581 ggaatttcaa taatagataa agataaaaat attatattag accaatatgg ttttaaaagt
     5641 aaagaaaaag caaactatga agatgttaac aatgcattaa aagatataaa aactaaaaat
     5701 ttaacacctt atacttatag aataccagat acaggggagc atgttatgag tatctcctta
     5761 ccacttaagg taaataatat aatagaaggt gttgttagat atacagtatc attagatgct
     5821 attgataatg caatattaaa gcaagctaca tggcttatat tagcaggaat atttatactt
     5881 attatagcta tactaataag tcttaaattt gctgagactc ttataaaacc attacgagag
     5941 ctaaagaaat ttgcaaatga attggctgta ggaaattata atataaaatt agaaaagatg
     6001 aagattgtag atgatgaaat tggtgattta gcacagacat ttgaacacat ggcacatgag
     6061 attgacaaaa gtgaaaaatt aaaagaggaa tttatatcat ctgtatctca tgaactaagg
     6121 actcctctta catctataaa ggggtggagt gaaaccttag gttatgaaag tataacaaga
     6181 gaagaactag acttaggttt aggcattata caagatgaaa cagaaagact tataaaacta
     6241 gttgaagaat tattagactt ttcaaggctt tcttcagaca ggataaaact tcatgttgat
     6301 atagttgatg ttgaaggttt aattgtaggt gttgtaaatc aacttaaagt taaagcagct
     6361 gaaaaagata tatcactttt atttgaattt gaaaatgaat ttatagagaa cattcaagga
     6421 gataaaaata gattaagaca ggttctcata aatttaatac aaaattcatt taaatttacg
     6481 agtcaaggtg gttacataaa agttgtagct tctcaagatg aagagataac aaccatatca
     6541 gtagaggata atggttcagg tatagaaaag caaaatttaa ataaagtgtt agataaattt
     6601 ttccaagaag attataataa ggctggtagt ggattaggac tagcaattag taatgaaatt
     6661 gtaaaacttc atggtggaag aatgaaaata gaaagtgaaa agaatgttgg aacaaaaata
     6721 acttttaata taaaaaataa atttgctaaa caagcataaa ttcttagaaa gaggattaag
     6781 atgagaaaca taaaaatgat agtagcatac gatggttcaa gatataaagg atatcaaaaa
     6841 ttaggtgaca ataatatgac tattcaagaa aaacttgaaa atgttttaag taaaatgact
     6901 aatgaaacag tggagataat aggttctgga agaactgata tgggagctca tgcaagagga
     6961 caagtggtaa attttagaac taattgtatg gattctttgg ataaaattca aaaatattta
     7021 tatgaatatt taccagaaga tatagttgta aaaactgttg aagaagttga tgaaagattt
     7081 cattcaagat acaatgtaaa atcaaagact tatatgtata aaattgacaa taataagtat
     7141 cataatcctt ttataagaaa atatgctact catgtaagta aaaaattaga tttagataga
     7201 atgagaaaag caagtgagta tttagtagga gaacatgatt ttactagttt tgcttcttct
     7261 aaatctaaga aaaaatcaaa tgtaagagaa atttattcta taaatataaa agaagatgat
     7321 aatgttatag aaatatatgt agaaggaaat ggatttttgt ataacatggt aaggataata
     7381 gtaggtgctt tgatagatgt tggtctaaaa agaagagctc cacaagatat taagtatatg
     7441 cttgaatcaa aagatagatg tcaatcatca gatactgcac cagctaaagg actatgtctt
     7501 tggaaagtaa catattaatc aattatataa aactctcttt aaaataaaaa taaaataaat
     7561 tatatagttg acataaatgg aaaaataaac tattatatat tttataagag agatataaaa
     7621 ctttatattt aaatcctgtg agaaagagag taacttctat gtgttttata gagagctgag
     7681 gatggtggaa gcttggcaaa taagtattag tgaaaagaac tttggaggat actaattgaa
     7741 tttagtatgt tagtaggtaa tctcgcctta agagaaagag tggataagtt tatcttatca
     7801 attagagtgg taacgcggat atatttcgtc tcttggtttt tccaagaggc gttttttaat
     7861 atatttaatt attagtgttt atctggtatg ttgaacattt actaattgat tagctaagtt
     7921 tatcaataag agtggtaccg cggatatttc gcctcttagt attttaaaat actaaggggt
     7981 ttttttagta ttaaaatgat tttatgtgag tctaaattct cagagtcatt caatattaag
     8041 aatttctata aaataaaaat tataatttaa gattgggagg aattaaaaat gaaggaaaaa
     8101 gtagtattag catattcagg aggacttgac acatcaatca taataccttg gttaaaggaa
     8161 aattatgaag atatagatgt tatagctgtt tgtggaaatg taggtcagga agataaaatg
     8221 gaagatgtat atgaaaaagc tttacaaagt ggagcatcaa aggcatatgt ggatgatata
     8281 agtgaagaat ttgtaactga aactatattt aaagctgtaa aagcagaggc taaatatgaa
     8341 ggaaaatact tattaggaac ttctttagca agacctataa tagctaaaaa attagttgaa
     8401 gttgctcata aagaaggtgc aaaatatata tgccatggat gtacaggaaa aggtaatgac
     8461 caagttagat ttgaagctac aattgcagct cttgacccaa ctataaaagt tattgcacct
     8521 tggagaattt gggatataaa atcaagagaa gatgctatag actatgctga aaaacataat
     8581 ataaaggtaa cagcaactaa agctaaaata tattcagtag atgccaacct ttggcatgtt
     8641 tcaacagaag gtggagatat agagcaccta gaaaatgaac ataaaaaaga tgtttataaa
     8701 caatgtgtag acccagaaga tgcatgtgat gtagctgaat atgtagaagt ttactttgaa
     8761 aaaggtgtac ctaaaaaggt aaatggcgaa gaattatctc cagttgcact gattcataag
     8821 ttaaatgagt tgggttgtaa acatggaatt ggtgttatag atatagttga gaatagactt
     8881 gttggtatga aatcaagagg tatatatgag actccaggag gaacaatcct ttatgaagca
     8941 cataatattt tagaaagtgc tactttagac aaagatacat tacatttcaa acaaatggta
     9001 tcttataaat acggagaact tatttacaat ggactatggt attgtaaatt aagagaatct
     9061 atagatgcat ttatggaaca aactcaagat aatgttacag gaacagtaaa agttaaactt
     9121 tacaaaggaa atataaaacc tgctggaata tttactgaaa atgctcttta tgatgaagga
     9181 atatcttctt ttggaaatag tgaactttat gaccacaaag atgcagaagg atttattaat
     9241 ttattcactt taccattaaa aatcagagct atgaaagctg gaaaataata aaataaatta
     9301 tttaagagtg tataaaaacg atttttaggt ttttatatgc tcttttttta tttgaattta
     9361 ttaaattata cagtaaatag tataaaaaaa tatattatag ttccaaatgg cttaataatg
     9421 tgaaaatata agacctaaaa tataaatttt ataaatacaa tagttattga ctctctcatt
     9481 gtaagagggt ttatactgaa tctaatgaaa atttatagtg aggagataga ttatatgaga
     9541 aaaataattg aaattaaaca tcattcacat gattatgagt gtatgtggaa tggtatagaa
     9601 gatttatata taaataaaac cggtgaatac cttccaaacg attttttctt tttactgtca
     9661 ggttttggtt cattttgtta tatgaaaaca aataaagctg atttgaaaag aatggtagct
     9721 ttgggagatg gaaggacaaa acagatgtat aaatttttag caccaattgt tggatttgaa
     9781 tacaaacatc atgagtttaa aaagtatgag caagcattaa agaaagcaaa atctgaaatt
     9841 gatgatggat atccagttat tttaggagct ttagatatgt attatctacc atattataag
     9901 aagctatatc ataaagaaca tattcctttt cattatatat taatggtagg atatgatgat
     9961 gaaggaatat atctgtatga ttgtggtaga acagaattat tacatctttc ttatgataaa
    10021 ttacgtgaaa gcatgaattg caattcccct ggattaagca aagaaaatac tatttgcaca
    10081 ataagaatga atacttcaaa gaataaatat aacattgcta gagaagctat tgctataaag
    10141 cgtgatatgt ttttaaatcc acctaaagga tttttgggat ataaaggttt tgaaaagttt
    10201 attaaagagt tgccacagtg gaacactgaa ttggataagg atgattatga taagattttg
    10261 accaatatgg ttatgttttt tggaacagta ccaacaattc caaatgcaat caaaggaatt
    10321 aaagaaccag atactgttca atttaaaggt ggatttgata aaatgagtag aatgctcaac
    10381 agtattgggg aggaatacaa aaacaactct tttatacaag tagctgatat ttttgataaa
    10441 actgctatta taattgagaa aatatccaat attataatag attacttaac tcaaacatgt
    10501 gatgatacag aacaacttcc gttattgttc tctgaggtct tagagcatat gaaaacagga
    10561 tatttaatct tggatgtata aaaaatatat tgttgaccct aacattacgt aatagtgtaa
    10621 tatgaaaata tagcttaaat atattagtag gtattgatgg ctgtaaaatt aaaatttgag
    10681 gtgtggatat gagaacagtg aaacaagttt ctgatttaac tggaataagt gtgagggcat
    10741 tacattatta tgatgaaata ggtcttttaa aaccaaataa aattacagat gcaggatata
    10801 gactttatga tgatgaatct attaaaactc ttcaacaaat tttatttttt aaggagattg
    10861 atattccttt aagagaggtt aaggaaataa tgtcaagcca atattttgat aaggttgaag
    10921 ctctaaaaaa tcaaaaaaaa ttacttattt taaaacgaaa gagactggat gaattgatag
    10981 agcttataaa tcaaactcta agaggagaag gaaatattga ttttaaagag tttgatatga
    11041 gcgagtattt taatgtttta gaagaattta aaagagagca taggaataag gtaattaaaa
    11101 tttatggtag tgtagaaaaa tacaatgaat atatagaaag agttaagtct aatgaagaga
    11161 aaattgctaa aatggctata aaacaatatg gaactattga gaagtttgct aaagctataa
    11221 aaacaaattt tagcagtgat atattgaatt taggagaaaa atttgataga tataaaaatg
    11281 attgtttaaa agaaaaacat ccaaaattaa aagaattata tagaaagctt gtagaagatt
    11341 taagtaggaa tccttcttca acagaccttc aagaaatagc aaaagcgata acagatatat
    11401 ctaaaaaaga ttatgaaatt ttctctatgg atactggtga tgacaactgg tattatatgg
    11461 ttcaaaatta tctagtaaat cctatgtgga tagaagaagt agataaaaag tatggaagtg
    11521 gagcaggaaa gtttattgga caagtactaa agacttatct aagagataga aaaccaaaga
    11581 taaatacatt gtatgaaaaa cttgtagaag atttgagtag agattgttct tcaagggaag
    11641 ttcaaagcat tgttgaagaa atagacaatg aaatgaaaag aagtaatgaa ttttataaaa
    11701 tagacaatgg tgaaagatac tttgattata tgtcagagct ttatttacaa gattctaact
    11761 atataaaagt aactgataag aattatggtg atggtgcatc taagtttatt ggagaagctt
    11821 ttaagattta ttttgataat aataattgtt gaaaatttgg aagtaaaaaa tatgtaagtt
    11881 ttataaaagc acaaaaataa ggatgtagat tagctccatc tatatcctta tttaaagtta
    11941 ttaaaatata tagtttacat caaacttaac actttcaata atatttccca aatatgtttt
    12001 tatactttca acttcttcta aagattttaa tttattttca gtataaattg ctttgtccag
    12061 aatattaaac cttaatccta tttcatcaaa gtactcatga tttaaaactt tgtaccaatg
    12121 gtttttttct ttattccaac tcgtagatat tgaatcagct tctttttgag ctttttccca
    12181 atcttcattt tctatagatt tttctaaaac agatatattg tctttatatc cttctgtaaa
    12241 atcatatagt ttattgttaa cgtaaaatcc aaataataca aataagatag tccatagtat
    12301 aacaaatgtt gtagatttca atattaatca ccctttttat tttgaataaa tattttgtca
    12361 ttttcatcta aaacacaaat aagaacatct ttaaaagttt ctatgtgatt agattttaaa
    12421 atacccatta accagttagt atctttttga gctatgtcta gatttttatt tattattcta
    12481 ccatccagta taagtgggat aggtaaatga ttaaaagctc ttggaggagt actattgtag
    12541 cttgatgctg gtacaacact tagattacca tcagtctcca aaatagcata ttgtacatct
    12601 tttaggttaa agtatccttg aatcctaagt tgttctaata attcatctat actaattctt
    12661 tcttttttaa gctctttata tacaattttt cctttatcta ttaatacaga aggtttacct
    12721 gataaaaatg aacttaattt tctgcttttt aatgataaga atgaaattag agtctgcatg
    12781 attacaagtc cagatattgc tgcaacacca tttatgattg gtatattatt attttccatt
    12841 gggacagatg caacttctgc aattaatagt gttataacta aatcaaaaca gttcatttca
    12901 gctatttcac cttttcccat gactctaagt gcaattagca ctgttatgta taatataata
    12961 cttcttatta atactactgt catatctatc aattactcct ttaaaaaata tttacactta
    13021 gttagtatat cctatataaa aaatttaatt caaagtataa aaaaccttaa ataattggat
    13081 tcaactattt aaggtttttt tgataatttt aatattaaat tttttatgga ttttctgtta
    13141 cttttattta ttttaataac agattcaaca aaactctttt tatctcctaa ttttaagata
    13201 aaatcaggat attcttcaaa ttcattgagt tttattaatt tgaaagaaga agatagattt
    13261 ttaaagttaa taattttaga gttattcact aatacacttc tttttttaaa ataaatttct
    13321 tttttactta tatttagtat agttaatatt tgtttaagat atattattat atcttctgga
    13381 gttctatcaa atttgagttc aaaattttca tcttcaaagt agggaatata atataaaggt
    13441 gttttgagag caggtaattt attatataat tcttcaagaa tatcgatatt tttaggggag
    13501 ttaaagaaaa agcatttaca tacattcatt aaatccacag catctgaaag tgagttatgt
    13561 ttaatatctc cttctacaga aaaaattttt ttcatattca gtaaagatat ttgcttagat
    13621 aagattctat tattgtatgt aatatgttga gatataaaag gttgaatgtc aatcatcata
    13681 ttaaataact ctaagcattt acctgtatat ttatacactc taaatgaagt tagaaggcca
    13741 gttatatcaa aatcacccca attatatatt gtaacatcat caaatttaca aaaccattta
    13801 taaaaatcat ccataacata taaaagatta ggagcattat tgatatctag ttggttcaat
    13861 ttggttaaat ttttacattt atttgaaagt attttattgt attttggctt aactaaagag
    13921 taataattat ctattgtatt aaattcgtta tcaactaaaa ctccaccaat agaaattaat
    13981 tccgcaccat tatattcttt tggtttattt ttattatcat ctagtatatt aaattcaaag
    14041 tctataaata acttgtattt cataaatcta aagcacatcc tcaggtaaat tatttattga
    14101 gcctaagcat cataagaatc aatagatata atgactctaa acatcaaagg ttcattacaa
    14161 cttttattta tttcataatt taattcaaat aaatctttta agatataatt tataagtatt
    14221 ctttcccaac ccatttttaa attaacttta gagtctatta atatattttc tatcttttta
    14281 aatatttcta tatttgctgg tattatactg agctcaatat tacttttaaa cgggttagac
    14341 tgtaaatctg ttataagtat attttttaat tcctctgtaa gttcagaaat taaactatca
    14401 cctatgtatt tttttatgat ttcctctcta atttctttta aatttagcaa tattagtacc
    14461 tgctacttac atagtagctt ttcctttcct tttaatacta ttattcattt ttgaatctta
    14521 tttagatata ttttaagatt aaaagtcttt agctatataa ttttttaccc atttttaact
    14581 tatattaaca tattacaggc ttatatctcc accctgttta cattgaggat ggagtatatt
    14641 gcctattttg ataaaaatga taggttgcta tttgcctatt cctttttctg ataatttttc
    14701 taacatatct ttggtcattt tatctaaatc ataactaaac ttaaagttcc attcttttac
    14761 tgcactacta ctgtctagag agttaggcca agaatcagct atactttgtc ttacagggtc
    14821 tacatcatat tctatcacaa aatcaggaat atattttttt atagaatcag ctatttcttc
    14881 aggagaaaag ctcatagcag taatattaaa agcatttcta tggatgagtt tatcagctgg
    14941 agcttcgatt aaatccacta ttgattgaag tgcatcaggc atatacatca tatccatttt
    15001 tgtaccttct gctatgtaag atttatatct cttatttttt aatgcctcat aatatatatc
    15061 aactgcataa tctgtagttc ctccaccagg aggcgtaaca taagaaataa gacctggaaa
    15121 tctaacacct ctagtgtcta ctccaaattt tgagtgataa taatcacata gtaattctcc
    15181 agaaacttta gtcacaccat acatagtatt aggtctttgt aatgtatctt gaggagtcat
    15241 attttttgga gaattttcac caaaagcagc aattgaacta ggtgtaaaaa atttacaatc
    15301 caattctttg gctatttcaa gaccatttat taatccattc atatttaagt tccaagcttc
    15361 tagaggttta ctttcagcaa ctgcagaaag taaagatgct agatgaatta tagtatctac
    15421 atcatatttt tttgcaattt caaaaaaacg attcttatct gtaacatcta atatctcaaa
    15481 tataccagat tcacatactg gattaccttc ttttactctc cttgaactag cgattacatt
    15541 ttgttctcca tactcatttc taagctttat tgttaattct gaacctattt gtcctaatgc
    15601 acctgttata agtatttttt tcaattttac aacctcctaa gggtttaatt tatgtagtac
    15661 tacttatttt tattttagca aatcttttgt ttaaatacaa agaattaaag ataataatat
    15721 acgacttaat tgaatgaagt gcagtaagtg cgtaaaaatt taaagctaat atataatggt
    15781 ctattgtata acaataaaag agtagattaa attattatat atagattgtg tgactaagtc
    15841 acagaaaata gagctaattt aatatataat aaaagcatgt caagtaaagc tttaaattaa
    15901 agtaacttta tataaaaagt aatataaatt gataaaatta taaatttatt tatagtgaat
    15961 ataggttgga ttttatagat acaaaaatag tatatatgat atgtagacca ttagcatgtt
    16021 taaagtagca aaacatataa aataaaattt gaaaggaagt ataaaaatga caagaccatc
    16081 aatatatcat agccaaggat acacttgtgc agaggcatta ataaagtctt ataatgagga
    16141 acacaacaca gatataccaa tctcaatagg aagtggtatg ggggttggaa tgaatgttgg
    16201 aagtgtttgt ggagctgtaa atgctgctgt tgttataata gggtatttaa atgggcgaaa
    16261 tagtaattta gatgaaaata tagcaaggaa gtattcaaga gagctattga atagagtaag
    16321 aaataaatac agctcagaaa tatgtgtatc tcttaaaaaa aataaagtaa cttgtgaaga
    16381 aataataaat ttcacatatg atgctctaaa tgatatgcta aaaaatcaaa aataattcta
    16441 agaaacaagt attttacatt cgatagaata cttgtttttt tatgatattt ttaataagta
    16501 ttagtttatt ataattttta aatcaatttt attatatttt aattgaaata atctactttg
    16561 aaaattacta actgacaaaa gaatatgatt atagtataga aaaaatgtaa attatttaat
    16621 attaagtgag agtatatgct tacctatata aaatcgtata aattaatggc taaatttata
    16681 aattataaag aaatatatag gaaaataaaa gaattaatta gtataatata aagttaaaaa
    16741 gtgaaaattt atataattta tttataaagt aaagaaaaaa tagaaatttg taataaaata
    16801 aaaagtttat gacataatat ggtataatta gtaaaaatat agagattgga ggaattgctt
    16861 tgtttataga tgaagaatta gaaggttata tattaacatg taaaatatct gaagacttta
    16921 aaaatatacc tgaatatagt gatgaagagt tttatgttac agtctataaa gatgaaagtt
    16981 ctgactctgg gtactatgct ttattagaaa ataaagaaga aagagttgta tgggatggag
    17041 aagttgttgc caataatatt tttaataacc tttggattgt aataaataag gttaaaactg
    17101 gataatagtc taattatata gtatttgtaa tattttaaat gaatttttac atatttgaga
    17161 tgagtaaata acaaagatta ttcgatactg gaggagtaat tcaatggaat atagttatag
    17221 taagatgaac ttaaaaaaag gggatattgt agaggtaaat ttagagaaac aagccaatgt
    17281 aatattgtta gaccatataa attatgtgaa atttaaaaat caaagaaatt atgattacta
    17341 tggtggattt gcaaagaaaa atccatgtag aatgagagtt cctaatactg gaacatggta
    17401 tctagtagta aatcaagatg gaaatagtgg aatagtaaac ttttcaatca atacaattca
    17461 aaattaattt tagaatattg gatattctta ctttttgtac tttttatata gttaaaataa
    17521 aattaataag caattattat aaaataatgt gggtgcctca aaatgaattt tataattcac
    17581 gagacactct tttttatatg aactcaaata tactttatca tttcaacaat gaaaaaaagg
    17641 cgtatctgtg ttttgagata gcctcattta tcatatatca tatatagaat tttaattatt
    17701 cttaactaaa attttttcca taggttatct gtacaattaa aatatttatt gttagcatac
    17761 agctccacct gtaaggtgta aaagagcctc tagtatctct tcttttcttt ccaaaacttc
    17821 atctgataaa agcatttcta taaatttatc agcaccgatt ctatcgatag ttattccaaa
    17881 acgttcacct gtgttaccct gttctctaaa taaaagaatt gctttttcaa ttaaagtcat
    17941 aagttcctct tttgaaaata atctatcaat ctgtgttcca ggacgaactg attttcccca
    18001 tttccctcct atatagactt tataaccaga ttctttttct tcaatactat caaaattaca
    18061 actttctata cacttaccac agttattaca tagattacta tctatttcaa gtttaccttt
    18121 atctgttaat ttagctgctt taacaggaca gacctctaca acagcacatt ttttacaacc
    18181 aacacaaagc tctgaatcat agtcaggtac acgttgacca acaattccaa gatcgtttag
    18241 gtcaggttta atacagttat tagggcatcc accaacacca attttgaact tatgaggtag
    18301 ttttacatca taccaatttt tgtaaaattc ttcgtgtatt tcagcagcaa gtgcctgtgt
    18361 atcagctaga ccatgaacac aaacagtacc tttacaagcc acaacaggac gaacccttga
    18421 tccagttcca cctgttatta gattttcgct agcaataaat tcacttagag cttcaatatc
    18481 ttcaaatttt acaccaggta attctactgt tagtctagta gtaaatgcta actgaccgtt
    18541 tccgaatttt tcagcagctt ctgcaatttt ttttgcttgt gatgcattta ttacaccatt
    18601 tacagttatt atacgtgctg caaaatgttc tccatccctg cttggtagaa aacctctacc
    18661 ttttacttcc tttttttgtt ctgctgtaac attactcata tttactcctc cattacctta
    18721 agatttggat aaaatagtat tccaccttca agaactttcg tatttgtata gccaaagcgt
    18781 cttaagcgat tttgcgccat atagctattt ttacctttag cacatataag aagaattttt
    18841 tcatccttag caaggccttc aatttctcca ttaatttcac caacaggaat ataacgtaaa
    18901 ctaggaatag aaggagcttt tgaaacatca agtttagtcc aactgtcaaa atcctcaatt
    18961 tcgtcaagta aaacactatc aagttctcca ttaagtttat tttgtaaaac atttactgcc
    19021 actacaaaag ggtgaatagc tgttgaaaaa ggtggtgcat atgcaaggtc catactttct
    19081 aatgaataaa ggtcagcgcc taaagttatt gcagtagcag cgatatctac tattttatca
    19141 accgctccag gtcccataac ttgaacacct aataattttc tagtattttt ttcagcaacc
    19201 attcttataa taaaattaga tgcacctgga taataatgag ctttatcatc agtagctatg
    19261 gttacagagc aaacattata accttctttt tcagctattt ctttacttag acctgttttt
    19321 cctgcattga gctcaggtaa ttttactact gttgttccaa gaacaccatt gtaagtttta
    19381 gattttccag atatattttg tgcgcaaatt ctaccctcat gatttgcaga tgaccccata
    19441 ggtgaccatg taggtttatt tgttaaagaa ttttttacaa aagcacagtc tccaactacg
    19501 tatatatctt tgtcatttgt aagcatatat tcatctgcaa taattgtatt attaggggca
    19561 aattctaaac ctgtgtcttt tagaaaacct gtgttaggac ggattccaac tgacataacc
    19621 actaaatctg ttttaatagc tctattttta gtacgaagtt tttcaacttt attttcacct
    19681 tcgataccta caacctggtc acctgtaaaa atcattatgc ctttatcagc catatgattt
    19741 tcaacgtatt cagcaaaatc agtatcaaat ccaggcataa catgttcagc catatcaaca
    19801 acacttacac gtatacccat tgcagaaaga ttttcagcaa tttcaagacc aatgaaacct
    19861 ccaccaataa caacagctct cttaataccg ttttcaatag aatctcttag ttttatagca
    19921 tcatttggag tacgcacaaa gaatactcca ggtaaatcta ttccaggtat tggtggtttt
    19981 attgggtctg cccctgtagc aattacaagt ttgtcatagc ttagattttc tttttctcct
    20041 gttgaaagcg ttttcaaagt tacttgtttt gcatttctat caatagatgt aacttcaact
    20101 ccacaacgaa cttctgctcc tgttagttta gaaaaacttt caggtgtatt tacgattaaa
    20161 gatgatttat cttcaattat cttaccaacg taatatggaa gaccacaacc tgcataagaa
    20221 atatcatcac ctttatttag tattgtaaca gagcaattat cacccatttc acgttttatt
    20281 tttgcagcag tttttgttcc agcagcaact ccacctataa ttaatacttt cacaatatat
    20341 cacactccaa tctactttat aactctataa taaaattata cttcttgaca atatatttgt
    20401 aaaatactaa aatattaata aaatcataag catttactaa tgtttgctaa ggttgtgata
    20461 atatgacaat tcgtagtctt gaaatctttg ttaaggttgc tgaatgtggt aaaatgagtg
    20521 aagtagcaag aaatatgtat attacccagt catcagttag ccaagctatt tcagaaatag
    20581 aaaaagagta tggtgttaag ctatttgata gaatttcaaa aaaattatat ttaactgagg
    20641 cagggaaaaa acttcttgga tatgggaggc atcttttagc tgtaaatgaa gaaatgaatg
    20701 attgtatgaa gcattgtgca aaaaatatcc gtataagagt tggggcaaca gtaactgttg
    20761 gtacatgtgt aataagtcca ataatgttag aattatacaa ggtaaatcct cttatagaac
    20821 ctgaagtatt tgtagaagat acaagactga ttgcaaaaaa attacttaat agtgaattag
    20881 atattgcgat tgtagaagga aaaattaagc acccagatat tgttacaaaa agcattatta
    20941 atgataatct tgtacttata tgttcacaca agcatgagtt ttataaaaga gactctataa
    21001 aagtttcaga actttcaaat caaccattaa ttatgcgtga attaggaagt ggtacaagag
    21061 cacaattaga aaagcaatta aaagaattaa aaatcccaat gaatattaga tggtcatgtt
    21121 ataactctga agctatatta agagcagttg tagataattt tggcattgca gttatatccg
    21181 aattacttat tgaggattat ttaaaaaagc atcttttgtg ggcttgtgat attgaaggta
    21241 ttaatttaca taggacattt gatattgcat atcatagaag caagtttttt acagaaaata
    21301 tttcagcatt ttttgatatt tcagtagaat atgggaaaaa acaagcgaga aagaagagta
    21361 gaagtgtaaa cagtttataa tatatctaaa taaaaataag agtatatcaa tatactctta
    21421 tttttattta gatatattag cctatattaa cgccaaaatt tttacacaat cctgcaagtc
    21481 catcctcaaa tccagaacct aaagcattaa atttccattc tccattatgt ctatatagtt
    21541 ctgccacaac tattgctgtt tctatgctaa aatcttcacc caattcatat ttaataagtt
    21601 cctcattagt ttcttcatta tatattctta tatatgaatt ttcaacttgt ccgaaatttt
    21661 gtcttcttgt aattgcttcg tttatagtaa cactaaatga tattttagca atattttgag
    21721 gtattttaga taagtctaca tttatttgtt catcatcacc atcaccaaca cctgttctat
    21781 tatcacccat atattcaaca gctccagaag cgtgttttaa attattatag aatataaagt
    21841 ctccatcatt cgttactttt ccatcagttc cagttaaaaa tgcagaggca tctaaatcga
    21901 aatcaaagcc accatcatat ttattaatat cccatcctag tcctactatt acttttttta
    21961 gaccagggtt actttttgtt aaacttactt tttgtccttt tgctaaagtt atacccattt
    22021 aaaccaactc ctcattataa attttttatt gtaattatta cttatatgta ttaattaatt
    22081 cacgaagctc ttcattttga ccaattccta aagcattaaa tttccattct tcatttctac
    22141 gataaatttc tcctgccaaa actgttactt taccagaaaa gtcttcttga agattatatt
    22201 taaataattc ttgcttggaa tttgaatcaa ttaatcttat aaaagagttg ttcatttgac
    22261 caaaatcctg gtctcttttt ttagctttgt atatagaaac tccaaaaact attttatgta
    22321 catattcagg tatgtcttct aaattaacaa ttatttgttc atcatcacct tcaccttttc
    22381 cagttaagtt atctccagtg tgtataatag ctttagaagg gtgtttaaga tttccaaaat
    22441 aaaccaagtc acctctaaca ttcattttat cttggttagt caatgctatt gcaaatgcat
    22501 ctaggtcata atttttttta cccaatttat tcatatccca tcctaagcct actactagat
    22561 tttttacagg attattatct tttactaagc taactctttg tcctttttgt aaactaacac
    22621 tcattttaag ttctcctttt tatattttta gttttattga tggtatcaag tataagaacc
    22681 atattagcta tattatatac gtatttaggc taagttggct atatttataa attatttatt
    22741 atataactac atgaatattt tataatttaa acatattgac attatatgct ttaaattatt
    22801 ttgtagtttt ataatattta aatttgtatt tgattgttat aaagtaaagt aagtaataaa
    22861 gttagactca ataattctaa aatttctaag catactatct aaatgagaac ggatttggaa
    22921 atacttgtat aaaacaataa ttgatacatt taatttttat cttaaaaaca acaattataa
    22981 tatataaata gcaagtataa tatataaata gcaagtataa tataacatat aaaaagttga
    23041 atttgtatat gttaattata cttgctattt atttgttagt tattctattt ttttaaatcc
    23101 attacctaaa gcttcatgta tatctgtaat tataataaaa gcattagggt cgacattttt
    23161 tactaatttt ttcaattcga caacttcttt cttttcaact actactaaaa gtacattttt
    23221 attttctcca gtatatgcac cttctccttt tagaatagtt gcacctctat ctaaatcttc
    23281 cattattttt ttacttatat cttttgaaag gtcagatata atagtgaatg atttggaata
    23341 attaaatcct tcaataagca agtcactaac ttttactaaa atgtataaag ccacagttga
    23401 ataaagtgca atttcaacct gtctatttac tatacctgat aaaacaacta caacgcagtc
    23461 tacaataccc attaaaacag gtatactaaa tgtaggaaaa tatttattta gtaggagtgc
    23521 cattaaatca gttccacctg tagagccatt tattctaaaa ataagtcctt gaccaactcc
    23581 cattaaaata gaaccagata taattgcaag atacatatca tttgttatat ctaatgtagc
    23641 catatttgca gtaagcttaa gtgctatggt aagaaaaatt attcctaaaa ccgttttaaa
    23701 acaatctttt tttgaaagta ttttgtaagc aaatatgaat aatggaacat ttaaaagtaa
    23761 attgacaatc caaagttcta caccgaataa tgaatttaaa acaactccaa gtcctgttat
    23821 accaccagca gcgattgcat gtggattaaa aaataagtta agagaaatgg ccattaatat
    23881 acagcctaca attaatatta aaatttctga aaaagttatt ctatcttttt tcaataacat
    23941 cactccttat atatagatta taatatatat tatatagttt ttccaatttt taataaaaga
    24001 aataaaaata tattataaaa acaatttgat tggaattttc agaaaattaa tgctataatt
    24061 aaaagtaaat ttaaagggag gttataaaat ggctaaatta actctagaac atataaaagg
    24121 aaatacatat tatattccac tccctacaat agtaggtgta tacgtagatg gtaaggatgc
    24181 aattttaatt gacagtggaa ataataagga tacagctaga caagttttaa gattattaga
    24241 agaacataat ttaaatccta aattaataat aaacactcat tcaaatgcag accatatagg
    24301 aggaaatgca tatttgaaga atcaaacaaa atgcaagatt gctacgacta aaattgaagg
    24361 atattttaca gaaaatccaa tattagaatc agcattttta tatggtggct atccaagtaa
    24421 agcactgaag aataaatttt tattagcaaa ggaaagtgaa gttgattata ttataccttc
    24481 aaatggcaaa attgtagata cagaacttga agcgatttct cttccaggtc attattttga
    24541 aatgattggt gttagaactc cagataatgt tttgtttgtt gcagatagtc ttacaccaga
    24601 gaatataatt actaaatatc attttttctt cctactggat atagattcac agtttaagac
    24661 actagaaaaa ttgagaatgt tagaggctga tttctttgtt ccaagtcact ctgtcaaaac
    24721 tacagatata aaaagtttaa ttaatattaa taagaagaag atggaagaaa taatagataa
    24781 tattaaaaag gtttgttatg agccagttat gattgatgaa gttattgaaa aaatgtgtga
    24841 tttgtacaat gtcaagcttg atgctaatca atatgtgttg gttggaagta caatacgttc
    24901 ttatataact tatttatatg agaataatat ggttgaatat atatttgatg gtggtaagat
    24961 gatgataaaa gctgcgtaaa tagtaataat tttcaaatta tatgatgaaa ttgttttagt
    25021 atgactgtta tttgaaaagt aatataaaaa aatttatata aataagctaa gatgattgat
    25081 ataaagttac tgataatttt aaggtaatat atacgttact ataatattat gttattattt
    25141 gtaaataagg ggataattta taagcaaatt aaagacctta atttacagga ggaatagtgt
    25201 atattatgaa taaaaaattc aaaaaattca gtaaaaaact aagtatatta attttagttt
    25261 tgactatgtt tctttttaat aatttaactg caaatgcaca gattaaagaa gattcaataa
    25321 taggaattga tagatatgaa acagcaggac ttatagcgga tagacaaact tatgatactg
    25381 taatattagt taatggagat aaaaatttat ctgatgggct ttcatcaagt ggattagctg
    25441 gagctattaa cgcaccaata cttttgacta aaaaaagtga aataccaaaa gctactttaa
    25501 atagactaga taataagagt ttaaatgttg taaaaaaagt atatataatt ggtggttata
    25561 atactattga aaattcagta gaaaaagata taaaaagaaa aggtattcaa gtggaaagaa
    25621 taaatggtaa caatagaatt gaaactagtt ataatgtagc taaaaaaata aatgaagtaa
    25681 gtagtgtaaa agaggtaatg cttacaaatg gatttgtagg agaggctgat gctatgagta
    25741 tagcacctgt agttgcaaaa aataaaggag ctataatact tacagatggg aaatcagttc
    25801 catttaagac tgaaggttta aatgtttacg ctataggtgg caaatctgcg ataaatgagg
    25861 atttagtaaa tgaaactaaa gcaattagaa tcggaggaaa tgatagattt gagaccaata
    25921 aaaaggtaat ggaaaagttt tataatggag ctacagattt ttatataaca aatggatatc
    25981 aattagtaga tgcactaaca ttatctcctt tagccaaaga aaaaccaatc gttttagttg
    26041 ctgatggaag taataaaggt attttaaaag gagctaaaag tataacaaaa gttggaggta
    26101 ttgatgccaa tatatataaa cagtgtttag atatgattaa atataataat atggatatga
    26161 ctccaaatat tttaaaacac ttaacttcag ttgaaggaaa tgaagtttct gaaatactta
    26221 tagaatcaga taatttaggt gtggttgttg aaaagacgaa taccaataaa tttgagtttg
    26281 attacgtaag tgtaactaat gaaaaaaatt gtactttttc tgtatataaa gaaaattctt
    26341 ctaataatat aaaaaatgga aagctagttg taagtgctaa gaaaaaaata gataagaaag
    26401 atagtgattt gcaagatatg aatggcgaca atatgataaa tgcaaataaa gataggatgg
    26461 taaatgtgat aaaaattgga gttcccgata aaatgtattc taattttaaa gtagatgttg
    26521 aaagtggttc agtagaactt tataatatta aaggaggagc tactgttgat gtcaatgacg
    26581 gaattgctaa cattgttgat aacaatgtaa caaatccttt caatataaat actaatagtg
    26641 ggatttcagg tgtaacagct gaaactatat catctgaaat aaaatttaga gcaaacaatg
    26701 gaattgtaaa tgtaacagct acaaatataa atggcaatat aagtttattt ggaagcgata
    26761 ataaagatac ttttgatgga atatttaaat taaatctgaa aaaagaacca tcaaatttac
    26821 acttgaagtt aattggaaat gggataaata aattgccaga aggttggtct aaagattata
    26881 ttttaggaaa tggacaacca attattgaag ttaaaaataa tggcataaat aatatatcaa
    26941 ttggagaata aaaacatatg aaaataaagt ttatatagtt ttaaaaatta tagaatttta
    27001 aaatatttat atatattctt taaataaaaa attatttttt aagtgataat taagtgttta
    27061 tcgataaggc tatggttgta aaaatcatag ccttattttt atattataaa taaattagag
    27121 tatttatcaa ttcatagtag ataaaattga aattaaatat aacaacaaaa aatgaaaatt
    27181 acaattaatt taatgttaaa gactcaatta tttggatgtg gaatggatag tacaagtagt
    27241 atataaaaag tattgactgg acaaataact atattttatt ttaaatatat aatataatgt
    27301 aataaaatat atcaaatgta gaaggtgata acctatgaaa aaaataacaa taaatgatat
    27361 tgctaacttg gcaggagtat ctaaaagtac tgtatcaaga tatctaaaca ataaagatat
    27421 cagtgattct actaaagaga aaataaagac aataatagat gaatatggat atgagccgaa
    27481 tgcatttgct caaagtttga gagctaaaaa aacatatttt attggtatta taacaccgtg
    27541 tttagattct tttgtaaaat ctaaaattat gatggcaata gatgaagaac tcaaaaaatt
    27601 aaaatataca tcactaatta ttaatactaa tagaaaaata aggtcagaaa tagatagtat
    27661 atcaaagtta gctagtctaa aagttgatgg aataatacta attggtaccg aaataactaa
    27721 agaacataag aatgaaattg aaaagttaga tataccgata gttgtagttg gacaaaaagt
    27781 tgatggtatc aatagcatag tgaatgatga ttatggagca ggctataaaa tgggtcaata
    27841 tattgctaat aaaggttaca aaaacattgt atatttaggt gttgatgaaa gtgatatatc
    27901 agttggttta aatagaaaaa atggagtact taatgggtta aaagataaag ggtatgatgc
    27961 aaaagtattt tatactgact ttgaccaaga aatgtctata caaagaagtg gagaaatgct
    28021 agaaagtgaa aatccagata taataatatg tgcaactgat aatatagcaa tagcaacaat
    28081 gaaagaaatc aataagagag gtaaaagtat accacaagat atatctgtag ctggatttgg
    28141 gggatatgat gtattgtcta ttataagccc taaacttaca acaataaagt ttgaaaataa
    28201 aaatgcaggt aaagtagcag caaatacgat agtaaattta atacaagaaa gaaaagaacc
    28261 actattaaag gagattaaat ttgaattaat agaaggtgaa agtacaataa ataaaaatta
    28321 agatacacat tacatatatt agtaatgtgt atttttttac taaaaaaact ataattctaa
    28381 aaattaggaa aacttttttg aaagaaatat tgcatctgaa ataaatttat gctaatcttt
    28441 aattaaaccg gtttcgaaac cggtttaatt aaaccgatag aatagcaaaa tgagtgcaaa
    28501 aataactgat tgtaacaaag tatcaaagta attattggag tacataggaa taaacaaaat
    28561 ataaaagatg tagcacattg tgtaataaga ctaagaattg ttttagatag caataataaa
    28621 gcaaatataa aattataaaa gaaaaggaat aaaaatatgt acaaagagcc taaatataga
    28681 accatattag aatcgacaaa agaagaatta aataaattaa gaaatatatc tttaaatgat
    28741 aaatataaac cactatttca tatacatcca caacatggac tacttaatga cccaaatgga
    28801 ttagcatatt ataatggtaa gtaccatgta ttttatcaat ggtatccata tgatgcaact
    28861 catggaatga agcattgggc ttatgtaagt tctgatgatt ttgtaaactg gaatagagaa
    28921 gatgtagcat taataccaat cgaaagttat gaatctcatg gagcatattc aggtaatgcg
    28981 atagaggtag atggaaaatt gcatatgtat tatacaggga atataaaata tagtgcagaa
    29041 gatagatatg catatcaaaa cttagctata atgaacaagg atggtaaaat aactaaatat
    29101 gaaaataatc caatagtaag tgaaatacca aaaggatata caggacatgt aagagaccca
    29161 aaagtattta aaagaaagga taaatacttt atgttacttg gagcacaaac aagtgataaa
    29221 aaaggagtta taatagtata tgaatctaaa aactctatag attggaattt taaaggagaa
    29281 ttaaatgtaa aaaacataga tgaagatttt ggttacatgt gggaatgtcc agattatatt
    29341 aatatagatg aaaaagatat attaatattt tcaccacaag gagtagaacc taaaggattt
    29401 gattatcaaa atatatataa tgtagtatat gctattggta atatggattt agataattta
    29461 acatttgaaa tagatacaat gaaagaactg gaaaaaggat ttgatttcta tgctccacaa
    29521 acatttataa aggactcaca aataatacta tttgcatggg ctggaatggg agaagtttta
    29581 taccctacag ataaaaacaa atgggcacat tgtctaactg ttccaagaaa attaaatata
    29641 aaaaataata agctattgca aatgccagta gatgaattaa ttaaattaag atatgatgaa
    29701 acaagtgggc aaaatactat aaagaataat attaatatta tagaaaatga tgaaaatgta
    29761 tatgagctta atattaatat aaaaaatatt gacagcaata aatttgggct ggaattattt
    29821 tcatcacaag atgaaggtgt aaaattagaa tttaacaaat taggaaacat tgtaacttta
    29881 gacagaagca actttaaaaa agttttcggt gttgaatatg gaacaaatag aaaagaatat
    29941 ataaatattg atgaaaatac taatatcaaa gtactagcgg atagaagtat tttagaaatt
    30001 tttataaatg aaggagaagt agtatttact agtagaatat ttgctaaaga aaattctaat
    30061 caaataaggg tttatagtga taaaatagta gagtatgagt atactaaatt taaattaaag
    30121 caaggtattg aattataaaa acacattata aggggtagaa aagatgaaaa aagtaattag
    30181 tataggagaa gctttaatag actttatacc taatcaaaat ggtggtaaat taaaaaatgt
    30241 atcagaattt agaagagtag caggaggagc tccagcaaat gttagtgctg ttgtagcaaa
    30301 gcttggagga aaatcgagtt ttatatctaa gttaggaaaa gatgcatttg gtgattatat
    30361 aatagatgta ttaaatgaag taaatgtaaa tactgattat gtattgagaa caagtaaagc
    30421 aaatacagga cttgcttttg tatcattaaa agaagatggt aatcgtgatt tctcatttta
    30481 tagaaatcca agtgcagata tgcttttaga agctgatgaa gttaaaaaag agtggtttaa
    30541 taattgtcat atcctacatt tttgttcagt agatttaata gatagtccaa tgaaattagc
    30601 acataaaaaa gcaatagaat acgcacttga aagtaatagt ataataagct ttgaccctaa
    30661 tataagatta cctttatggg atagtgagca atcttgtaaa aaggcaatat cagaattttt
    30721 accttttgct catattgtta agatatctga tgaagaatta gaatttataa caggagaaaa
    30781 tgatatagaa aaatcattac ataaactctt tgtggggaat gttgaactag tattatatac
    30841 aaaaggaaaa gatggagtag atgcatacac taaaaaagta aaaggtatgt gtaaaggggt
    30901 taaagtaaat gcaatagata caaccggagc aggagattct tatataggtt catttttata
    30961 caccttattg tataaacaaa taacattgaa tgatataaaa gaaatgaaac aagaaacgtt
    31021 aaatgagtat ttagaatttt ctaattatta tgcagctcgt agtacaacta ttaaaggtgc
    31081 gataagttca tatgcaacaa aggaagaaat aactgaattt ataaaaaatt tatagtagga
    31141 atgagtaata ggttttgaag gaagtaacat gtaaagagtt tttaaggatg tttactatga
    31201 aaataattaa catataaaaa gcaataagga ggctgtctca aaatagagat aagcctcttt
    31261 atcattgttg aaatgataaa aaatatttta attattgtta ctttttagaa gaatatatat
    31321 cttattttaa ataagcaaat tatgtaagat ttatttatat tatcaatact ttacttggag
    31381 tattatgtct ttagcagtta tctaattcaa ttgaattaga taaaatataa taaatttgtt
    31441 ttgcaaattg acgagaatgt tcttcttttt ctttattcat aaaattaggt ttacctaaat
    31501 cacgttcaac aagcattcct atatatttta actgtgaatg cttacagtag cgcttcatac
    31561 cattttccca aatatcagca cccttttcag gtctatatcc acatgttgta ataagtgcta
    31621 ctgattttcc tgcccataga gcggggcctt tctcattacc atagtattta ttcattccat
    31681 acaccaatct gtcaagtagt gctttcattg gagaagtgca gtaccaagaa taaattggag
    31741 tagcaaaaac aattaaatca cacataagaa ttttcttgaa aattttctga acatcatctt
    31801 tacaataaca tccaaaatca aataagttat cctgacactt tttacaagca atacatggct
    31861 ctattttaca atcatataac catattactt caatatttga attataattt tctaattcct
    31921 caataaaagg ttttaaaata gcagcagtat tacctttttt cttaggactt cccataagaa
    31981 tgcatacttc catattgata cctccacagt attttaaaat agtttttagt gcttaatata
    32041 gttttaggat actatgagaa tacgttaaca acaatacact ttgtatattt gtaataaaat
    32101 tgaaacaaat tgttttatca ataaaagttg gaatgtatct ataattaaaa atattttagg
    32161 tatgttttta catatatgtg tgtagttaac tatggtaatt tattgtaaaa ttatcttata
    32221 ggattataat tgcttaattt atgattatat ttaaaatctt ggaggtaaaa aatatgtact
    32281 caaatgaaga ctataaattt atagaagaaa atgcaccatg ttatatatat gatgagacta
    32341 atataattga aaaatgtaag gaattgaaaa ataatatttt aggttttgat tttttatatt
    32401 ctataaaaac aaatccatat aacaaaattg taaaaagcat tgcagagcaa ggttttggag
    32461 cagatgcttc ttcaactaat gaagttatga tttcatcaaa atgtggagtt tctaaagatg
    32521 atatttatta ttcatcacca ggcaaaacag agattgatat agaaactgtt ttagataaat
    32581 gcataattat tgcagatagt attacagaaa tagaacttat aaataagata tctaaaaagc
    32641 ataatcagaa agttaaaata ggaataagaa taaatcctga ttttactatg gatgatgata
    32701 gtgctaaatc aagcaaattt ggtatagata tagaagatat gagcagtgtt tttaagatgt
    32761 taagtatgtg tgattatgta tctgtagttg ggattcatat acatattaaa tctcaggtat
    32821 tggattttga aaagttagga cattattatg aaaaatgttt ttttatagct ttaaatttaa
    32881 ataaaagtaa gtatattaat attgaattta taaattttgg tagtggactt ggagtagctt
    32941 atgataatct tagagaaaat ccacttaatc ttaaagagct ttcaaaatta actcaaaaga
    33001 ttgcttttac taattcaaac acactaaaat caagacttct tattgaatca ggacgtttca
    33061 tagtttgtaa atcaggtaat tattatgtta aaattgtaga tattaaagta tcaagaggga
    33121 aaaaatatct tattgtaaaa aatggactta atggatttat gagacctatt atttctaatt
    33181 taattgaaaa tattacttgt ggagtagaat tagaaggtca agagccatta tatacatcaa
    33241 atcatgcctt ttctctttca gtattaaata atttaaaaga agaagagtat gtaaatgttg
    33301 ttgggaatct ctgtacatca ttagatgtaa ttgcaaataa tgttaaattg aaaaaagcaa
    33361 acataggaga tttgatttca attagtaatg ctggaagtta ctcctattct ttatcaccgc
    33421 tattattttc aagttatgaa tttcctaaac aatattataa aagaaaagat ggatatattg
    33481 agtaaacaat agcataaatt aataaactta gtattattga aaaaatttaa aatgcagggg
    33541 gtgtaattct atatggaatt gtggaaaaga aatttatttg tatgttggat aggtatgttt
    33601 tttagttcta tagggatgag ccaaatagca cctatacttc cattatatat taaacagttg
    33661 ggggtaactg atgttagttt aatacaacag tattcaggta taatttttgg atgtaccttt
    33721 gtagtagcag cattcttttc gccaatttga gggaaagctg ctgataaata tggaagaaaa
    33781 tcaatgcttt tacgtgcaag tattgggatg ggaatagtta tatttaccat ggcgtttgtt
    33841 caaaatgttt atcagttatt aggattaaga atacttcaag gagtttttac gggttatgct
    33901 actgcatgta ctactcttat tgcaacacag acagataaaa atcattcagg atgggctttg
    33961 ggtactcttg caactgcatc aactactggc tcattaattg gtccaacagt aggaggatat
    34021 atagaatcaa tcttaggtct taaaagtaca tttattataa caggaggatt gttgtttgta
    34081 tcatttatca tctctatttt atttgtagta gataatttta aacctaaaga aataaaagaa
    34141 tcaatttcta tacctaaaga acaattaaat atattgccaa ataaattttt aattgcatct
    34201 atttttgtaa ccacattcat tactcaacta gctttatact ctatagaacc aattgtaacc
    34261 atatatatat cacaacttac aaattttgca tcaaatgtag ctttaatagc tggattgaca
    34321 ttttctgcat caggattagc aaatttatta gcagctcaaa aacttggaaa aatgtcggat
    34381 aaaataggtc ctcaaaaagt attgttaatt tcacttttat gggctgggat tatatttata
    34441 ccacaagctt ttgtaaaaac tgcatggcaa ctgatgttgt tgagattttt attagggttg
    34501 tcagttgcag ggcttaatcc ttctgttaac agcttgctta aaaaaattgc accagaagaa
    34561 tatgtgggaa aaatatttgg atataatgca tctgcccagt atataggctg ttcatcagga
    34621 gcatttttag ggggacaaat atcagcacat ttaggtatta gaacagtatt tttttcaaca
    34681 agtttattgt tgtttaggaa tgcactttgg atgtataaat ttactggatt gtttattaaa
    34741 gataagacta aataatcata ctatttacat ttctaatttt atgatgtaaa ataaatattt
    34801 tttatgtaat ttatcataga attatataaa aaataaccct atggtaaaaa ttggaaaata
    34861 ttattatcga caaaatcttt gacttaaaaa ctatattgcg atagtattaa tttaataaaa
    34921 gtttgagagg ggcgtaaggt tatgacaatt tcaaaaatac cacaagcaga attaaaagtt
    34981 atgaagttta tttgggaaaa aaatgataca gtaacatcaa aagaaactat tgaagctatg
    35041 gaacgaaaat atggttggaa acaaacaact acattgacac ttttatcaag acttgttaaa
    35101 aaacattttt taagtgcaga aaaaatagat agatacactc attatacaat agttgttgga
    35161 cataaggagt atttaagtgt tgagacaaaa gattttttaa gcaatataca cgacaattct
    35221 ttacagagtt taatttcagc actacatgat gatgaagttg ttgataaaga tatgttggat
    35281 ttatttgaaa gtcattttaa aaatttagaa gaagagtaat tatatcagga tagaactcta
    35341 agaatttaat taatctatgg ctgaaaacca taaatggtat tatttaatca gctactcact
    35401 attcataaag taatattaaa taggttctct aattagtaaa atgcctaaaa taattggaat
    35461 agggagtatc aagttaaatg ataccactta tttaaataag aaatgatttt taaaatttaa
    35521 aataatttta taactt
//
LOCUS       DAEPIN010000043        35535 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.043, whole genome shotgun sequence.
ACCESSION   DAEPIN010000043 DAEPIN010000000
VERSION     DAEPIN010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 35535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35535
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.043"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            440..1447
                     /gene="asrA"
                     /locus_tag="KR250_002241"
     CDS             440..1447
                     /gene="asrA"
                     /locus_tag="KR250_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfite reductase subunit AsrA"
                     /protein_id="HBH0068174.1"
                     /translation="MKLKLDSSKFNEGLNYLKKDYKIFAPVLIPFKGTFSDTDVIRYK
                     EVNAFEEMEFAKKANFSPKDVVLPINQVLFYFTEKEFKESDLDNKKILIFLRACDING
                     IKRLDEIYLNNGEEKDYFYKNIRDKVKFALVGCKESFRNCFCVSMDSNKTDNYSIGLN
                     IEGDTLYLDIKDSEFEVFNGEATEFNVDYVTDNLISVDVPENIDSNEMIGNSIWDEYD
                     TRCIGCGRCNFVCPTCSCFTMQDIFYKENENVGERRRVWASCQVDGYTDIAGGNSFRK
                     KQGERMRFKTMHKIHDFKKRFGYHMCVGCGRCDDACPQYISFSKCIEKINDLVISKEE
                     V"
     gene            1448..2239
                     /gene="asrB"
                     /locus_tag="KR250_002242"
     CDS             1448..2239
                     /gene="asrB"
                     /locus_tag="KR250_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfite reductase subunit AsrB"
                     /protein_id="HBH0068175.1"
                     /translation="MNPYIPVSAKILDIVKHTDKEWTFRVNCNTKGVLPGKFYEISIP
                     KYGESPISVSGYGEDYIDFTIRNVGKVTSELFNYKEGDSFFIRGPYGNGFDVSLYEGR
                     EIVVVAGGSALAPVRGIVEYFYNNKEKCEKFKLIVGFKSPKDILFADDLKRWSKKLDI
                     LVTVDGAEEGYSGNVGLVTKYIPELDMKDINNTSIVVVGPPMMMKFTVEEFLKRDLDE
                     KNIWVSYERKMCCGIGKCGHCKMDDTYICLDGPVFDYSYAKNLID"
     gene            2252..3223
                     /gene="asrC"
                     /locus_tag="KR250_002243"
     CDS             2252..3223
                     /gene="asrC"
                     /locus_tag="KR250_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite reductase subunit C"
                     /protein_id="HBH0068176.1"
                     /translation="MIRDLNTKKVMKNAYRITKTKYETSLRVRVPGGLIDPESLAVVS
                     KIASEYGNGQVHITTRQGFEILGINMEDMEEINKIIQPVIEKMNINQSEKNAGYPAAG
                     TRNIAACIGNKVCPKAQYNTTEFAKRIEKAIFPNDLHFKVALTGCPNDCIKARMNDFG
                     IIGMALPIYEKDRCVNCGACVKKCSKISVGALKTENNKVVRDKDKCIGCGECVLNCPT
                     NAWTRDEKKYYRLAIMGRTGKKNPRLAEDFLLWVDEDSIIKIILNTYRYVEKYIDKDA
                     PGGKEHIGYIIDRTGFMEFKKWALEGVELPEITKMYENIYWSGIKYL"
     gene            3280..4056
                     /locus_tag="KR250_002244"
     CDS             3280..4056
                     /locus_tag="KR250_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HBH0068177.1"
                     /translation="MHKETLDKLTNAAINKINLLNTSKVKYLVSSAFAGLYVGIGILL
                     IFTIGGLLTDAGSPMTKIVMGLSFAIALSLVIMTGTELFTGNNMVMSAGMLNKGVSIK
                     DTSKIWAYSWVGNLIGALVLGIIFVGTGLVDKGPVAEFFANTAASKASMPFTALFFRG
                     ILCNILVCVSVLCSFRTNSDTAKIIMIFLCLFAFITSGFEHSVANMTIYSVSLFSPTI
                     STVTIGGAIYNLVAVTLGNIVGGALFMGLGTYILGKEKLN"
     gene            4327..6345
                     /locus_tag="KR250_002245"
     CDS             4327..6345
                     /locus_tag="KR250_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBH0068178.1"
                     /translation="MTKKLVISKTKLFIFIIAFISIFIYLFGSKNTLIGVGIVTAMLT
                     LLERDLTISPIKNLLKYLAINIILGILSFFAVQNMYLGVLLNFIALFIIGYVFSYDLK
                     RAVYVPFGLMYIFMVSIPVGKSEFPMRLSALAVGAVIIMIAQFVMNRNRMKNVGDKEL
                     ISICDELLEKISLLKNTINDDSSIIKSSMRKIDSCNYRINSISKNLKMVIFDNRKDDF
                     YISIRGIDIMNILFSLERISLILERYKKDSKEFEDEDIKNILEESSINSKSDVLVVAT
                     KEINYIKMCLENKDTITDKEILGFRDYVIAQDTKNINLKEIYSVLENLYEFLVDYKKV
                     KSRDEKKAERKSKIPHEFKRLSIYKKNFNLNSIRFSYAVKIALATAVAGFIMDYFHLR
                     DGRWIMLTVFSLTQPYAENCIQRSRKRIEGTFIGAVIFIVLFSIIKDSTLRSLIVLLA
                     GYINSYVVDYRKLMVCVTVSALGSAVVMGDPNVLTISRISYVALGAIIALIVNKFILP
                     YDAKTGYQHVIEMYKGIVKNIIDEVNKSIENVADVYYIKNLLLIPSLIEDRLMLINAI
                     YKDKHQEDFLENQKLLISNMYNLYINVNKNKIKDEDVEKILRDTNYISNYNVDKYDEG
                     RSVILESIVNTKSLGDKIICLNLLQTLNGVKEMYRISNITKVSIKEAA"
     gene            6560..7417
                     /locus_tag="KR250_002246"
     CDS             6560..7417
                     /locus_tag="KR250_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBH0068179.1"
                     /translation="MSKDVKVKRNKKYDTFSITMELERPLLYDEVENKDDKNNNRKFN
                     IKNKIFKGIGILFVLILLGFFIWINKTYEPQKLAKEALVSNSKVEVTVNENISFTPKG
                     RKVSKGLILYPGAKVEIESYAPLARKIAESGYEVVIVKMPLNLAILGTNKAQKVMDSY
                     NNIEHWVIGGHSLGGVAASNFARDNKLIEGVVFLASYPMGDELKELGKKVISIWGSKD
                     GVVNFKSLVESKQKLPDDTTYVEIEGGNHAQFGDYGKQKGDNDAIISQEKQLNITANS
                     IIKFLKNIS"
     gene            7514..8383
                     /locus_tag="KR250_002247"
     CDS             7514..8383
                     /locus_tag="KR250_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBH0068180.1"
                     /translation="MEYDMPLYRPPSEAYSLIIQVTLGCSHNKCTFCSMYKSKKFKIK
                     PLEVIKNEIDIFRRYYKHVERIFLADGDALIIPMEKLRDIILYIKEVFPECERITMYG
                     SPKSIEKKSDDELKELRKLGVKMIYLGLESGSNEVLEDIKKGFSSEQLIKVGRKVKKS
                     GIKLSATVIAGLGGTKKTHQHAVDTGKMLGAISPDYVGVLSLMVEPNTELYDLLKAGE
                     FTILEDKAVLQEIKEMIKNIDANEKIVFRSNHASNYANLKGVLPDDKQRLIDEIDYYL
                     SNQKLKREEYRRL"
     gene            complement(8754..9386)
                     /locus_tag="KR250_002248"
     CDS             complement(8754..9386)
                     /locus_tag="KR250_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HBH0068181.1"
                     /translation="MTIPSSNKVYPRSNDYQTVYLKNVITRENIEVGDYTIYNDFYND
                     PRDFEKNNVLYHYPINNDKLIIGKFCSIACNAKFLMTSGNHSMKSKSTYTFPIFYEEW
                     KLDINHITDAWDNKGNIIIGNDVWIGYDSIIMSGVNIGDGAIIGTRAVVTNDVPPYSI
                     VGGVPAKIIKKRFNNDTISKLLEIKWWDWPYKKIQSNIKHIQSGNIDKLV"
     gene            complement(9458..9811)
                     /locus_tag="KR250_002249"
     CDS             complement(9458..9811)
                     /locus_tag="KR250_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase DNA-binding domain-containing protein"
                     /protein_id="HBH0068182.1"
                     /translation="MSTEKRRDNKDRILRTGESQRKDGRYAYKYVYSFGKPQFVYSWK
                     LVPTDKPPKGKRDDISLRDKEKAIQNDLDDSIDTIGKKMTVCQLYAKKNGLRKNIRLN
                     TKKGRHYLMKILNKE"
     gene            complement(9889..10092)
                     /locus_tag="KR250_002250"
     CDS             complement(9889..10092)
                     /locus_tag="KR250_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HBH0068183.1"
                     /translation="MNNTDVPIWEKYTLTIEEASKYFRIGEKKLRKLADENLDSGWVI
                     MNSNRIQIKRKQFEKIVDTLDFI"
     gene            complement(10482..10712)
                     /locus_tag="KR250_002251"
     CDS             complement(10482..10712)
                     /locus_tag="KR250_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH0068184.1"
                     /translation="MKPTKEVLPFTIILLAVHGDVNAINGILKHFEHYIIRLSQKTLF
                     DEYGNPYIHVEEETKRMLGTKLITAILRFNLS"
     gene            complement(10714..11121)
                     /locus_tag="KR250_002252"
     CDS             complement(10714..11121)
                     /locus_tag="KR250_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBH0068185.1"
                     /translation="MVDEKTIEHQFDSFCKTVLRNYARDIYDENKRRNDYLVSLESLS
                     LAELSKLSILDDYDSNYICMVSYDYNIRIEDVLMAQAIGKLTKRKQDIILLSFFLNMT
                     NADIATLMDLAENTIHYHKTNALKELKELMEEH"
     gene            complement(11481..11930)
                     /locus_tag="KR250_002253"
     CDS             complement(11481..11930)
                     /locus_tag="KR250_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBH0068186.1"
                     /translation="MELIEWNYAYITDLVEFANDKEIAKNLRDNFPHPYREQNARDFV
                     SFCLATPDVKQLSYAIFYQERAIGSISLTFEEDTSINKNAELGYWLARNYWGKGIATN
                     AVKEICKIAFEKYNLNKIHANVYSYNRGSSKVLEKCGFKIEEYCHNS"
     gene            complement(11944..12870)
                     /locus_tag="KR250_002254"
     CDS             complement(11944..12870)
                     /locus_tag="KR250_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0068187.1"
                     /translation="MSDILSRITNKIGDKNIVDKLSALSKSDLNSLLLEVFDRQANTL
                     TATDILKSYQLNRFTIPSGIDPEEIHALESKLLRKAFNMDIKTIMLSPSAPLGSCSVF
                     GSVDQYNVVSALRGTETLADPSNMLAIIIADKLKRKEENNTIPIHMATTARVIRAQNF
                     VGKGLYSHFGLYCMVSSGIDTGSYTCEKMLLEKHLNYYKDILSEIENVRFSIILRKRN
                     GYKDNDGFFDRMVETIKLIFPNIELSIHNDDVDNNYYKGINFKLSVKQGNETVEIVDG
                     GFVDWTYQMLGNKKERCLISGIGLERFLVAVS"
     gene            complement(12995..13399)
                     /locus_tag="KR250_002255"
                     /pseudo
     CDS             complement(12995..13399)
                     /locus_tag="KR250_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433844.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oxalate:formate antiporter"
     gene            complement(13549..14169)
                     /locus_tag="KR250_002256"
     CDS             complement(13549..14169)
                     /locus_tag="KR250_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBH0068188.1"
                     /translation="MHLIDFMNEDIKKKLVKKTYKKKETILFAEQENEYVILMIDGIA
                     EAFILNVKGNISTIHLYRSGSFFGEMEQFNDGKKPVEIVAFTDCNVYKLHRDDFLNWL
                     RGDFEATKFLIREISSKLVVNAELIEELSSLSVKERLLRCISLHYYRSELNLLTKKQL
                     ASEVNAPIRSINRAINECVQEHLIAYENKRFSVINQREVLKNLPTT"
     gene            complement(14268..15536)
                     /locus_tag="KR250_002257"
                     /pseudo
     CDS             complement(14268..15536)
                     /locus_tag="KR250_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            15628..16443
                     /locus_tag="KR250_002258"
     CDS             15628..16443
                     /locus_tag="KR250_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase family protein"
                     /protein_id="HBH0068189.1"
                     /translation="MVKLSDAKSWAIRMSEDGCAYKSIDNYKRSLKASFFMAIQDDCI
                     RKNPFNFQLSDVLEDTTEPKIALTPEQEMGLLSFMESDKTYCKYYDDVVILLETGLRI
                     SELCGLTLELDMKNKAIMVDHQLLSDTKIGYYVEVPKTTQGKREIPMTERAYQSFERI
                     VERRKNAKAEPIIVDGYQNFLFLKQDGLPKQATHYQSMLRALVKKYNKTHEDKLPKIT
                     PHTFRHTLCTNMANKGMSPNILQYIMGHKNITMTLGYYAHGSFSSSRAEMERL"
     gene            16917..19961
                     /locus_tag="KR250_002259"
     CDS             16917..19961
                     /locus_tag="KR250_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SrtB-anchored collagen-binding adhesin"
                     /protein_id="HBH0068190.1"
                     /translation="MKKILKRLCTGVLAFATVVTALPTTLVHAESKQYWTESEERVGI
                     VEKVMNDGSIGSTFNEGHLTVEGEDAYCIDINTDFKNGYKTRADASSRMSADQISDVA
                     LSIEYVKQYTDNHNGISKNHAYLLRQLVVWQRLSVHLGWQCDNVRASYDEIPKATQDE
                     VFAGAKAFVKENKGRYECGGYIYSGEGQELGQFWAKLNVGNAKLQKTSNNTSITNGNG
                     NYSIAGATYGVFSDKDCTKQIATLTTDDSGNTEVAEVKATTVYIKELSAPLGYKVDTT
                     VYSLKVEAGKTATLKVSDTPKVTDTLIELFKIDMETQKANPQGNASLEGAEFTWKYYA
                     GFYNKDNLPAEATRTWVTKTTAETDSNGTTHYITKLADAYKVSGDSFYMQDGKAFLPL
                     GTLTVEETKSPTGYLLDGAYMQANGSEEQIKALYLTQITEDGDLAVLTGSNQFSVSDK
                     VIRGGVKIQKRDLETKDTKPQGSATLKDTAFDIISLNDNAVLVEGKIYKKNEVVKTIH
                     TDIEGIASTSADLLPYGNFRIVEKQAPNGYLTDGAKPIDFTITENGKIVDLTDEAHSI
                     YNQIKRGDIEGVKIGAGTHKRLADVPFRITSKTTGESHVVVTDDNGQFSTSSKWASHK
                     HNTNAGKTSEDGVWFGTSEPDDSKGALPYDTYIIEELRSDSNKGMELIPPFEIVVSRN
                     NLVIDLGTLTDEYEKEITIHTTATSKDGEKTILAGKEVTIVDTVKLDGLTKGTKYQLK
                     GWQMLKEENAEIIIDGKRVESDYTFVADDEEMKVEISYTFNASELGGKNLVTFEELYD
                     LSNPDEPMKVAEHKDIEDDGQTVLITERIIKIHTTATDKDGNKELEAGKDVTIIDTVT
                     LEGLEVGTKYKLVGWQMLKEENAELLINGKRVESDYTFIADSETMKVEVTFTFDATSL
                     DGKQLVTFEELYDLSNPNEPKKVTEHKDIEDEGQTITFKEKPEVPEKPETPTTPEKPS
                     RPTDSPKTGDNTNVMAFLVMLLTSAGGLAGTYLYKRRKMKKS"
     gene            20197..20523
                     /locus_tag="KR250_002260"
     CDS             20197..20523
                     /locus_tag="KR250_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005337695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBH0068191.1"
                     /translation="MELKYVVPNMAQTFGNLEFAGENKIEQQRINGRFTPVSRSYNLY
                     SDVQRADDIIVTIPAKAGEKHFKPEQRVKLINPKITADGKAVKGRGFTNYILTADDML
                     PAEESK"
     gene            20538..20921
                     /locus_tag="KR250_002261"
     CDS             20538..20921
                     /locus_tag="KR250_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBH0068192.1"
                     /translation="MRLANGIVLDKEKTFGVLKFSALRHEVRAENDDGSTSEEITRRT
                     YDLKCSVQERMIQVSIPADIPVKDYPYNAEVELVNPVADTVANASYRGVNVDWYIKAD
                     DIILKNKGGQVGNPQNHAPQGQPKK"
     gene            21019..21504
                     /locus_tag="KR250_002262"
     CDS             21019..21504
                     /locus_tag="KR250_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006776881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0068193.1"
                     /translation="MAKEKGSFKEFLSAIAPEHQAFVEKLNTKLIEQGCDLVIKEAKS
                     GYTASYQLEKKTVMNWVFRKTGVFARIYGDNAGKYEDIIASLPADMQKKMTTSRDCKR
                     LIDPNACSDTCVKGFVYALNGDTHKKCRNDGMLFVLTNETAEHIAGLVCAEVNVRKSA
                     L"
     gene            21621..23015
                     /locus_tag="KR250_002263"
     CDS             21621..23015
                     /locus_tag="KR250_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBH0068194.1"
                     /translation="MKMILNKGHRIRASDKNLVYHFSIGTLLFVFIAVILLLNMKQLM
                     RTDWEHFSLLDNGLTLSTYNFITLLIATGVCALVAFLYYRFCYDSFKKLLHRQKLARM
                     ILENKWYEADTVQDSGFFTDLQSRSREKIIWFPKIYYQMEKGLLHIRCEITLGKYQDQ
                     LLRLEDKLESGLYCELTDKTLHDGYIEYTMLYDMIANRITIDEVKAENGCLRLMKNLV
                     WEYDALPHALIAGGTGGGKTYFLLTLIEALLHTNAVLYILDPKNADLADLGTVMGNVY
                     HTKEEMIDCVNAFYEGMVQRSEEMKRHPDYKTGENYAYLGLPPCFLIFDEYVAFFEML
                     GTKESVSLLSQLKKIVMLGRQAGYFLIVACQRPDAKYFSDGIRDNFNFRVGLGRISEL
                     GYGMLFSSDVKKQFFQKRIKGRGYCDVGTSVISEFYTPLVPKGHDFLQTIGSLAYARQ
                     DGTATCEAKGDGTD"
     gene            23217..24416
                     /locus_tag="KR250_002264"
     CDS             23217..24416
                     /locus_tag="KR250_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HBH0068195.1"
                     /translation="MNETDFLTALKEKRCAYGVSQTRLAIMANISREHLSRIEAGKVT
                     LTEDMKHTLLEAVEKFNPNNPMFLLFDYVRIRFPTMDIKHIIKDILKLNIGYMLHEDY
                     GHYKYTEHYHIGDVFVYVSQDEEKGVLLELKGKGCRQFESYLLAQERSWYDFFTDALV
                     EGGVMKRLDLAINDRTGLLDIPELIRKCENEECISKFRSFKSYGSGELVKHNETDKYG
                     MGHTLYIGSFSSEVYFCCYEKNYEQYAKLGIPIEEVPIKNRFEIRLKNERAYYAVVEL
                     LTHYDAELTAFSIINQYIRFADKEPDKRKSDWKTNVRWAWFIGEGRPPVKLTTQPEPY
                     TLERTLNWLQRQVAPTLKMLKQIDKDNQTEYLETIEKQAKLTERHYQIIKQQTATVEE
                     MVANVKE"
     gene            24424..24558
                     /locus_tag="KR250_002265"
     CDS             24424..24558
                     /locus_tag="KR250_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017143882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HBH0068196.1"
                     /translation="MWELLKDLLLVSFGMGIGVVLMCILTVGKEADRCMEEIKEREDN
                     "
     gene            25300..27210
                     /gene="ltrA"
                     /locus_tag="KR250_002266"
     CDS             25300..27210
                     /gene="ltrA"
                     /locus_tag="KR250_002266"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HBH0068197.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNHILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            27377..27538
                     /locus_tag="KR250_002267"
     CDS             27377..27538
                     /locus_tag="KR250_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0068198.1"
                     /translation="MAIVVIGIYLGFKREFSKLIGFLVVALVAVGLVFNAGGVKDVLL
                     ELFNKIIGA"
     gene            27623..28225
                     /locus_tag="KR250_002268"
     CDS             27623..28225
                     /locus_tag="KR250_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBH0068199.1"
                     /translation="MDKVVIYIHGKGGNAEESSHYKPLFSDCDVVGLNYTAQYPWEAK
                     EEFPSLFDLICKHYKSVQIVANSIGAYFAMNALSDKQIEKAYFISPIVNMEKLIADMM
                     YWANVTEDELRDKKEIKTTFGETLSWEYLCYVKENPTTWKIPTNILFGEKDNLTSYAT
                     ISEFTKQTNATLTVMRNGEHWFHTEEQMKFLDEWINKSAR"
     gene            28322..28618
                     /locus_tag="KR250_002269"
     CDS             28322..28618
                     /locus_tag="KR250_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005339688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBH0068200.1"
                     /translation="MNDIFRDMQAKVGCENISDLPSYKRKVWHEMKQLNLADYEEKQL
                     EDFSKYVFGMSCQTLKDVMKQQKGREEQCRKQGYWWKRKEQLAKKQHHTGSTCR"
     gene            28537..29040
                     /locus_tag="KR250_002270"
     CDS             28537..29040
                     /locus_tag="KR250_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBH0068201.1"
                     /translation="MQETRVLVETKRTTGEETTSYWFDLPIEVAEFEEKLGVSTESQD
                     YRIIEKLLPYADEVHEHTSVYQLNELDYMYRQLSSDMQEEYTMLHTVYENLEALYICR
                     NVITLYPDCKSMIDVARQKLMNDPTFKHLSEDCQEYYFDFETYASHLQEHGKFLVTEH
                     GIFELPE"
     gene            29043..29546
                     /locus_tag="KR250_002271"
     CDS             29043..29546
                     /locus_tag="KR250_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBH0068202.1"
                     /translation="MIDDMAVYIANLGKYNEGYLVGAWFTFPIDEEDVKEKIGLNEQY
                     EEYAIHDTDNFPIEIGEYVSIDELNEMYEMIEELPDYIVDCLDEFIGHYGTLEEVVEH
                     KDDIYYYPDCETMTDVAYYYIDELQALGDIPPSLQNYIDYEAYGRDLDMGGCFIATSR
                     GMCEIPY"
     gene            29664..30053
                     /locus_tag="KR250_002272"
     CDS             29664..30053
                     /locus_tag="KR250_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009274602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBH0068203.1"
                     /translation="MKKIRSYTGIWNVEKVLYAINDFNLPFPVTFTQITWFVITEFII
                     ILFGDMPPLSMIEGAFLKYFGIPAFLTWFMSQKTFDGKKPYSFLKSQITYALRPKITY
                     AGKAVKLQKEIFNETITAVRSVSYVPD"
     gene            30040..32490
                     /locus_tag="KR250_002273"
     CDS             30040..32490
                     /locus_tag="KR250_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBH0068204.1"
                     /translation="MFPIKYIDNNLVWNKDNEVFAYYELIPYNYSFLSAEQKFIVHDS
                     FRQLIAQSREGKIHALQIATESSIRSMQEQSKRLVTGKLKEVAYQKIDEQTEALVSMI
                     GDNQVDYRFFLGFKLMVTEEQLNLKNLKKSAWLTFKEFLHEVNHTLMNDFVSMPNDEI
                     NRYMKMEKLLENKISRRFKVRRLEINDFGYLMEHLYGRDGIAYEDYEHQLPKKKYKKE
                     TLIKYYDLIRPTRCVIEESQRYLRLEHEDKESYVSYFTVNAIVGELDFPSSEIFYFQQ
                     QQFTFPVDTSMNVEIVENRKALTTVRNKKKELKDLDNHAYQAGSETSSNVVDALDSVD
                     ELETDLDQTKESMYKLSYVIRVSADDLDELKRRCDEVKDFYDDLNVKLVRPAGDMLGL
                     HSEFLPASKRYINDYVQYVKSDFLAGLGFGATQQLGENTGIYMGYSVDTGRNVYLQPS
                     LASQGVKGTVTNALASAFVGSLGGGKSFCNNLLVYYSVLFGGQAVILDPKSERGNWKE
                     TLPEIAHEINIVNLTSDKDNAGLLDPFVIMKNVKDAESLAIDILTFLTGISSRDGEKF
                     PVLRKAVRSVTQSETRGLLHVIDELRCEDTAISRNIADHIDSFTDYDFAHLLFSDGTV
                     ENAISLDNQLNIIQVADLVLPDKDTTFEEYTTIELLSVSMLIVISTFALDFIHSDRSI
                     FKIVDLDEAWAFLNVAQGEILSNKLVRAGRAMQAGVYFVTQSSGDVSKESLKNNIGLK
                     FAFRSTDINEIKQTLEFFGIDKEDENNQKRLRDLENGQCLLQDLYGRVGVVQIHPVFE
                     ELLHAFDTRPPIQRNEVE"
     gene            32490..34682
                     /locus_tag="KR250_002274"
     CDS             32490..34682
                     /locus_tag="KR250_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBH0068205.1"
                     /translation="MKERIQSAFTKKKIFHFLKMALFVVALSLILLSLLGTVAHATGL
                     VDDTINAENLYSKYPLSNYQLDFYVDNSWAWLPWNWLDGIGKSVQYGLYCITNFVWTI
                     SLYLSNATGYVVQEAYKLDFINDMADSIGQSIQTLAGVTSSGFSTSGFYVGFLLLIIL
                     VVGLYVAYTGLIKRETSKALHAVINFVVVFILSASFIAYAPDYIKKINEFSSDISTAS
                     LDLGTKIMLPNSDSEGKDSVDLIRDSLFSIQVEQPWLLLQFGNSNAEEIGTDRVEALV
                     SASPENEDGKTREEVVKTEIESNDNNNLTIPQVVNRLGMVFFLLFFNLGITIFVFLLT
                     GMMLFSQILFIIFAMFLPISFLLSMIPSYESMAKQAIVRVFNTIMTRAGITLIVTVAF
                     SISSMFYNISTDYPFFMVAFLQIVCFAGIYMKLGDLMSMFSLNANDSQSMGRRIFRRP
                     YLYMAHRARRMERRLAGAVTAGGVAGAVAGSTVSNARTTRNNSAKDNRGNTSSSMGQR
                     AGSKVGAVLDTKNKVKDKANAMKETVKDIPTQTAYAVHSAKEKAKTSVSDFKRGMVQE
                     QQSRQSGRLEKQEQHKKNIADKRMELQKAQEARQARKTDGSATTGATRPHERPVTASA
                     IPKTNVEKTQEVKRPATTSTSKAGEPVKTNSVKERPLSSGTSDGKIPQSAQTVHSKNV
                     EKVVSHETHQNYKAERTTKAQTVEQAKRTTEHTEKNRNLVTKKGQKKK"
     gene            34679..>35535
                     /locus_tag="KR250_002275"
     CDS             34679..>35535
                     /locus_tag="KR250_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HBH0068206.1"
                     /translation="MKLKHIAIIGSLFPILFSIVLFFGVLMSADSDDENSNFSSGITG
                     MNLSAEVLKHQPMVEKYAKEYGISEYVNVLLAIIQVESGGTAKDVMQSSESLGLPPNS
                     LDTESSIKQGCKYFASLLSSCKNQGINDLNVAIQSYNYGGGYVGYVAGKGKKHTFNLA
                     ESFAREKSGGKKVTYTNPIAVAKNGGWRYGYGNMFYVELVNQYLTVPQVSGELAQKVM
                     NEALKYQGWKYVYGGSNPNTSFDCSGLTQWCYGKAGISLPRTAQMQYDATQHIPLSQA
                     KAGELVFFHS"
BASE COUNT        12373 a         5682 c         7009 g        10471 t
ORIGIN      
        1 atagtataat tatgcagaaa taactcgaat ttcactactt atgaagagat tgaatattaa
       61 tgaatctaaa tcatttgcat tacttaagag ttttgactaa agttgaacac catacaaaag
      121 tagctactca attgtttata ccccaaccaa gcttaagtaa tgttatatag attctcgaaa
      181 aagaatttgg tatcttttta tttgaaaaat tgagtagata gtagttttat tgtacttaat
      241 gattgtattt aagtgattag atttgaaatt gaataatgta gataataaaa aaatattaac
      301 agaacttaat aaactccatt aatatttttt tattattttt gaaactgtga taattatcac
      361 ggaagcatag aaaataaagt gttaagattt aaacaagata gtaagtaagt aaaactaaaa
      421 tataagaaag gtgataaaaa tgaaactaaa attagattca tctaagttca atgaaggtct
      481 gaattatcta aaaaaagact acaagatatt tgcaccagtt ttaataccat ttaaaggtac
      541 tttttcagat actgatgtta taagatacaa ggaggtaaac gcttttgaag aaatggaatt
      601 tgctaagaaa gcaaactttt caccaaaaga tgttgtactt cccataaatc aagtattgtt
      661 ttattttact gaaaaagagt ttaaggaaag cgatttagac aacaagaaaa ttttaatttt
      721 cttaagagct tgtgacataa atggtataaa aagacttgat gaaatatatc taaataatgg
      781 tgaagaaaaa gattattttt ataaaaatat cagagataaa gtaaagtttg cacttgtagg
      841 gtgtaaggaa agttttagaa actgtttttg tgtgagcatg gattctaaca aaactgataa
      901 ttattcaatt ggattaaata tagaaggaga tacactgtac ttagatataa aggatagtga
      961 atttgaagta tttaatggag aagcaactga atttaatgtt gattatgtaa ctgataattt
     1021 aatatctgta gatgtgccag agaatattga ttcaaatgaa atgataggaa attcaatatg
     1081 ggatgaatat gatacaagat gtataggatg tggtagatgt aattttgtat gtccaacatg
     1141 ttcatgtttt acaatgcagg atattttcta taaggaaaac gaaaatgtag gtgagagaag
     1201 aagggtttgg gcttcttgtc aagtagatgg atatacagat atagcaggtg gaaattcttt
     1261 tagaaaaaaa caaggcgaaa gaatgagatt taaaactatg cataaaatac atgattttaa
     1321 aaaaagattc ggatatcata tgtgtgttgg ctgtggaaga tgtgatgatg cctgtccgca
     1381 atacatatca ttttcaaagt gtatagaaaa aataaatgat ttagtaatta gtaaggagga
     1441 agtataaatg aacccatata taccagtaag tgcaaaaata ctagatatag ttaaacatac
     1501 tgataaagaa tggactttta gagtaaactg caatacaaaa ggtgtattgc ctggaaagtt
     1561 ttatgagata tctataccta aatacggtga aagtccaata tcagtatcag gatatggaga
     1621 ggattatatt gactttacaa taagaaatgt aggaaaagta actagcgagt tgtttaatta
     1681 taaagaagga gattcttttt ttataagagg tccttatgga aatggatttg atgtatccct
     1741 ttatgaagga agagaaattg tagtagtggc aggaggaagt gcattagccc ctgtaagagg
     1801 aatagttgaa tatttttata ataataaaga aaaatgcgaa aagtttaaat taatagtagg
     1861 atttaaatca ccaaaagata ttttatttgc agatgattta aagagatgga gtaaaaaact
     1921 tgatatttta gttactgtag atggagcaga ggaaggatat agtggtaatg taggattagt
     1981 aacaaaatac ataccagaat tggatatgaa agatataaac aatacttcta tagttgtagt
     2041 tggacctcca atgatgatga aatttacagt agaagaattt ttaaaaagag atttagatga
     2101 aaagaacatt tgggtttctt atgaaagaaa aatgtgttgt ggaataggta aatgtggaca
     2161 ttgtaaaatg gatgatactt acatatgttt agatggacca gtatttgatt attcatatgc
     2221 taaaaatctt attgattagg gggtgaagtt tgtgataaga gatttaaata ccaaaaaagt
     2281 aatgaaaaat gcttatagaa ttacaaaaac aaaatatgag acttctttaa gagttagagt
     2341 acctggaggt cttattgacc ctgagagttt agctgtagtt tctaaaattg caagtgagta
     2401 cggcaatgga caggtgcaca taactacaag acaggggttt gaaatattag gaataaatat
     2461 ggaagatatg gaagaaataa ataaaattat acaacctgtt attgaaaaaa tgaatataaa
     2521 tcaaagtgag aaaaacgcgg gttatccagc agcaggtaca agaaatatag cagcttgtat
     2581 aggaaataaa gtctgtccaa aggctcaata taatacaact gaatttgcta aaagaataga
     2641 aaaagctata tttccaaatg atttacattt taaagtggct ttaactggtt gtccaaatga
     2701 ttgtataaaa gctagaatga atgactttgg aattattggt atggctttac caatatatga
     2761 aaaggataga tgtgtaaatt gtggagcatg tgtaaaaaaa tgttctaaga tatctgttgg
     2821 agcattaaaa acagaaaata ataaggtggt aagagataaa gataagtgta taggctgtgg
     2881 agagtgtgta ctaaattgcc caacaaatgc atggactaga gatgagaaaa aatactatag
     2941 attagctata atgggtagaa caggaaagaa aaaccctaga ctagcagaag attttctttt
     3001 atgggtagat gaggacagca taataaagat aatacttaat acttatagat atgtagaaaa
     3061 gtatatagat aaagatgcac caggaggaaa agaacatata ggatatataa ttgatagaac
     3121 aggatttatg gaatttaaaa aatgggcact tgaaggtgta gagcttccag aaataactaa
     3181 aatgtacgaa aacatatact ggagtggaat taaatatcta taaattgagt aaaacatata
     3241 tttgaataaa ttaaaattta atagaaggaa gagggagtta tgcataaaga aactttggat
     3301 aaattaacta atgcagctat aaataagata aatttattaa acacaagtaa agtaaaatat
     3361 ttggtatctt ctgcgtttgc aggactttat gtaggtatag gtatactttt gatatttact
     3421 attggaggac ttttgactga tgcaggttca cctatgacta agatagtaat ggggttatca
     3481 tttgcaatag cactaagttt ggttataatg acgggaacag agttatttac tggaaacaat
     3541 atggttatgt ctgctggtat gttaaataaa ggtgtaagta taaaagatac ttctaaaata
     3601 tgggcataca gttgggtagg taatttaatc ggagctttgg ttttaggcat tatatttgta
     3661 ggaactggtc tagtagacaa aggacctgta gctgaatttt ttgcaaatac agcagctagt
     3721 aaagcatcca tgccatttac agctcttttc tttagaggga ttttatgtaa tattctagta
     3781 tgtgtatcag tgttgtgttc gtttagaact aatagtgata ctgcaaaaat aattatgata
     3841 ttcttatgtt tatttgcttt tataacatca ggttttgagc atagtgtagc aaatatgaca
     3901 atttatagtg tttcattatt ttcaccaaca ataagtacag ttactattgg aggagctatt
     3961 tataatttag tagctgtaac acttggaaat attgttggag gagctttatt tatgggtctt
     4021 ggaacttata tattaggtaa agaaaagctt aattaaattt attgtataaa ttataactct
     4081 cttgataaat acatttttag tattgttgag agagttattt ttatattttt gaaaaaatat
     4141 ttgaaacaaa aaaaatatgg gagcatacga aaatatatcg gttatatata taaaaaatac
     4201 gtttatatat gaattatata gttctaaata taaaaaaagt taaagtccta ccgtaaaaaa
     4261 gcgcattttg aataagataa caaatatgat aaaattggtc ttgttgaaat ttttaggggg
     4321 gatatgatga caaagaaact agtaatatca aaaacaaagt tgtttatttt tataatagca
     4381 tttatttcta tatttatata tttatttgga agtaaaaata cacttatagg tgtaggtata
     4441 gtaacagcta tgctaacact cttggaaaga gatttaacta tttctccaat taaaaattta
     4501 ctgaagtatt tagcaataaa tataatctta ggaatactat cattttttgc agtacaaaat
     4561 atgtatttag gagttttatt aaattttata gcattattta taatagggta tgtatttagt
     4621 tatgacttaa aaagagcagt atatgtaccc tttggtctaa tgtatatatt tatggtaagt
     4681 atacctgtag gaaaaagtga atttcctatg agattgtcgg ctttagctgt tggagcagtt
     4741 ataataatga ttgctcaatt tgttatgaat agaaatagga tgaaaaatgt cggagataaa
     4801 gaactgatat ctatatgtga tgaattatta gaaaaaatta gccttttaaa aaatactatt
     4861 aatgatgata gttctataat taaatctagt atgagaaaaa tagatagctg taattataga
     4921 ataaattcta tatctaaaaa tttgaaaatg gttatttttg ataatagaaa agatgacttt
     4981 tatatcagta ttagaggtat agacataatg aatattttat tttcattaga aagaataagt
     5041 ttaatcctgg aaagatacaa aaaggatagt aaagaatttg aagatgaaga tataaaaaat
     5101 attttagaag aaagtagcat aaatagtaaa tcagatgttt tagttgtagc aacgaaagaa
     5161 ataaactata ttaagatgtg tttagaaaat aaagatacca taactgataa agagatatta
     5221 ggatttagag attatgttat tgctcaggat actaaaaata taaatttaaa agaaatttat
     5281 agtgttttag aaaatttata tgagttttta gtagattata aaaaagtaaa atcaagagac
     5341 gaaaagaaag ctgaaagaaa atcaaagata cctcatgaat ttaaacgact atcaatatat
     5401 aagaaaaact ttaatttaaa ttctataaga ttttcttatg cagtaaagat tgctttagct
     5461 acagctgtag cgggttttat tatggattat ttccatttaa gagatggaag atggataatg
     5521 ttaactgtct tttcattaac acaaccttac gcagaaaatt gtatacaaag gtctagaaaa
     5581 agaatagaag gtacatttat aggagctgta atatttattg tattattttc aataataaaa
     5641 gatagtacat taaggtcgtt aatagttctt ttagcagggt atataaactc ttatgtagtt
     5701 gattatagaa aattaatggt atgtgtaact gtttcagctc ttggttcagc agtagttatg
     5761 ggagacccaa atgttttaac aataagcagg atttcatatg tagcattagg agcaattatt
     5821 gcattaatag taaataagtt tatattacca tatgatgcta aaacaggcta tcaacatgtt
     5881 atagaaatgt ataaaggaat agttaaaaat ataattgatg aagtcaataa gtctatagaa
     5941 aatgtagctg atgtttacta tataaaaaat ttattattga taccatcact tattgaagat
     6001 agacttatgt tgataaatgc tatatataaa gataaacatc aagaggattt tttagaaaat
     6061 cagaaattac taataagtaa tatgtataat ctatatataa atgttaataa aaataaaata
     6121 aaagatgaag atgttgaaaa aatattgaga gataccaatt atatttcaaa ttacaatgta
     6181 gataagtatg atgaaggaag aagtgtaatt ttagagagta ttgtaaatac taaaagtcta
     6241 ggtgacaaga taatatgtct aaacttactg caaactctaa atggtgtcaa agaaatgtat
     6301 agaataagta atataactaa agtttcaata aaagaagcag cttagatatt aaattataga
     6361 atagccatat atttaaaata aagtatatgg ctatttgttt tataaaatac taaaagaaaa
     6421 taatatataa aaaacaaaaa acatatattt atgaatttta tatgaaaata gttaaaatat
     6481 tgattttttt cattggttga aaaaacaatt tacttaggat aaactataat agtattcaaa
     6541 tattatgggg gttagattta tgagtaaaga tgtaaaggta aagcgtaata agaaatatga
     6601 tacatttagt ataactatgg aacttgagag acctttactt tatgatgagg ttgagaacaa
     6661 agatgataaa aataataata gaaaatttaa cataaagaat aagatattta agggaatagg
     6721 tatactattt gtattgattt tgcttggctt tttcatatgg ataaataaaa catatgaacc
     6781 tcaaaagctg gcaaaagaag ctctagtcag caatagtaaa gttgaagtaa ctgtaaatga
     6841 aaacatttca tttacaccaa aaggaagaaa agtttctaaa ggtttaattt tgtatccagg
     6901 agctaaagta gaaatagaat cttatgctcc acttgcaaga aaaatagcag aaagtggata
     6961 tgaggttgta atcgtaaaga tgccacttaa tctagctata cttggaacta ataaggcaca
     7021 gaaagttatg gattcttaca acaacataga acattgggta ataggaggtc attcattagg
     7081 aggagttgca gcatcaaatt ttgcacgtga taataaatta attgaaggcg ttgtattttt
     7141 agcttcttat ccaatgggtg atgagttgaa agaattagga aaaaaagtta tatctatatg
     7201 ggggagcaaa gatggagtag tcaattttaa aagtcttgta gaatcaaaac aaaaacttcc
     7261 tgatgataca acttatgttg agattgaagg aggaaatcat gcacaatttg gagattatgg
     7321 aaaacaaaag ggagataatg atgctattat aagtcaagaa aaacaactta atattactgc
     7381 caatagtatc attaaatttt taaagaatat atcttaaact aaaatttaaa aaatataatt
     7441 ttataagtta ttatttaaag taaatattaa taatataata gaatgaatat tttattacaa
     7501 gggggatttt aatatggaat acgatatgcc actttacaga ccaccaagtg aggcttatag
     7561 tcttataata caagtcacac taggatgttc tcataacaaa tgtacgtttt gtagtatgta
     7621 caaatctaaa aaatttaaaa taaagccact agaagtaata aaaaatgaaa ttgatatatt
     7681 tagaagatat tataagcatg tagaaagaat attcttagca gatggagatg cattgataat
     7741 accaatggag aaattaagag atattatctt atatataaaa gaagtttttc cagagtgtga
     7801 aagaattact atgtacggct ctcctaaatc tatagagaaa aaatctgatg atgaacttaa
     7861 agaattaaga aaacttggtg ttaaaatgat ttatctaggg cttgaaagtg gaagcaatga
     7921 agtattagaa gatattaaga aaggttttag tagtgaacaa ttaataaaag ttggtagaaa
     7981 agttaaaaaa agtgggatta aattatcagc cactgtaata gcaggtcttg gaggaactaa
     8041 aaaaacacat cagcatgcag ttgatacagg aaaaatgtta ggagcaattt caccagatta
     8101 tgtgggtgta cttagtttaa tggtagaacc taatactgaa ctttacgatt tacttaaggc
     8161 aggagagttt acaatactag aggataaggc tgtgttacaa gaaattaaag agatgataaa
     8221 gaatatagat gctaatgaaa aaattgtatt tagaagtaat catgcatcaa attatgctaa
     8281 tttaaaaggt gtattacctg atgataaaca acgtctaatt gatgaaattg attactatct
     8341 aagtaaccaa aagctaaaaa gagaagaata tagaagacta tagtttttga tatatcaatc
     8401 tatattgata ggtaaatact aagattattt agatacaaaa aatcatgcta cattaatagt
     8461 tgcttactat tgatgtagca tgattttttt attaaaaaac aaaatggtat attcatatat
     8521 aatatatcaa taataatgaa aatgtgaact agtttaatag aattttacct attataataa
     8581 aaacaggaat ttaaataata aaaagtaaag taacttttta ttaagtaact ttacttttta
     8641 ttttatttaa tgttgtatta aatttatata tattaaaata atatacaaat tttgaaaaag
     8701 ctcttataaa atgattatta aagtaaagtt taatataaat aaattataaa atattaaact
     8761 aatttatcaa tatttcctga ttgaatatgc ttaatgtttg attgaatttt tttatatggc
     8821 caatcccacc atttaatttc taataatttt gaaattgtat cattattaaa tctctttttt
     8881 ataatttttg caggtactcc tccaacaata gaatacggtg gaacgtcatt agtaactact
     8941 gctcttgttc caataattgc accatcacca atatttacac ctgacataat tatagaatca
     9001 taacctatcc acacatcatt tccaattata atgttaccct tattgtccca agcgtccgtt
     9061 atatggttga tatcgagttt ccattcttca taaaagatgg gaaaagtata tgtggatttt
     9121 gatttcattg agtgatttcc actagtcatt agaaattttg cattacatgc tattgagcaa
     9181 aacttaccaa ttattaattt atcattgttt atagggtaat gatacaatac attgtttttc
     9241 tcaaaatctc ttgggtcatt ataaaagtca ttatatattg tgtaatctcc aacttctata
     9301 ttttcccttg ttattacatt tttaagatat acagtttgat aatcattact tcttggatat
     9361 accttatttg aacttggaat agtcatattt aaatctctcc tttaatatat attatgtttt
     9421 tgactattcc actactacaa tacatctctt cacagaatca ctccttgtta agaatcttca
     9481 taagataatg gcgtcctttt ttagtgttaa gtcggatatt ctttcttagt ccattctttt
     9541 tagcgtataa ctgacaaact gtcattttct taccaatagt gtcaatgcta tcatcaaggt
     9601 cattttgtat tgctttttcc ttatccctta atgatatatc atcacgtttt ccctttgggg
     9661 gtttatcagt aggtacaagt ttccaagagt aaacaaattg tggtttacca aatgaatata
     9721 catacttata tgcgtatctt ccatcttttc tctggctctc tccagttctt aaaattctat
     9781 ctttattatc tcgtcttttt tcagttgaca tgataagaag ctcctctcaa taaccgaatt
     9841 gagcttgata cgtcatatga atattataac atataaggct caatttcatt agataaagtc
     9901 aagtgtatca acgattttct caaattgttt tctctttatt tgaatgcgat tgctattcat
     9961 aatgacccag ccagaatcaa ggttttcatc agcaagttta cgtaattttt tctctccaat
    10021 acgaaagtat tttgacgctt cttcaatcgt caaggtatat ttttcccaaa taggtacatc
    10081 agtattgttc atagaatgta tcactccttt ctatgttcgt agttcagaag tagatagatg
    10141 gctttggtaa gctccattgg tattttaacc aatcccattc ttatgggcag aaccgtgtca
    10201 tacaggtgta tcattatact gtggtacagg ttacggcaaa acaaccgttc caaaggttat
    10261 tcacaaatca ctatatagta gctctcttga ttaaggtcat ggcgtatagt atttgttggc
    10321 tatctgtatc acaaacgtat ctatctactt tattcagttg tcaaagaaca gtcggcattg
    10381 atttgtctat aaaaaatcac aatagcttat gggcttttat agacaaataa ttagtgattc
    10441 tccatgtacc aaatagcttg atacatggag agagggcgag attaagacaa attaaatctc
    10501 aaaatagcag tgattagttt tgttcctagc attcgctttg tttcttcttc tacatgaata
    10561 tagggatttc cgtattcatc aaataaagtt ttctgtgaaa gacgaatgat atagtgttca
    10621 aaatgtttaa gtattccgtt tattgcattt acatcgccat gtacagcaag taaaataatt
    10681 gtaaaaggta atacttcttt agtaggtttc atttcaatgt tcctccatga gttctttcaa
    10741 ctcttttagt gcatttgttt tgtgataatg tatggtattt tctgctaaat ccatcaatgt
    10801 tgctatgtca gcatttgtca tgttgagaaa gaatgaaagt agaattatat cttgttttct
    10861 ttttgtaagt tttccgattg cttgtgccat taatacatct tcaattcgta tgttgtagtc
    10921 gtaagaaacc atacagatgt agttagaatc ataatcatct aaaatactca atttacttaa
    10981 ttctgcaagt gacaaagatt cgagagatac caaataatca tttctgcgtt tattttcgtc
    11041 ataaatatct ctagcatagt tacgcaaaac tgttttacag aaactgtcga attgatgttc
    11101 aattgtcttt tcatcaacca taagatttca ccctctttcc atcgcaagga gttaaataac
    11161 cttgcttact cctatcacgg tgggagggcg ttaaatcttc gataaatttc aaaaaaaaga
    11221 gaaaaaaata cacactctgc ctatagggca aaaatgtgta ttctttaaaa cgtatttaat
    11281 ttatattggt tgtaagccac caccatattg atgtatggaa atatgcattg gctagctttt
    11341 cataaaaata caaagatgca taagaaatta atagagggga aaaacttatt ctgttattaa
    11401 aaagtaggtt aattctaata aatgctattg cggtcgaatt aaactatata catggcaatc
    11461 aatcatgttt ctattcttta ttatgaattg tgacagtatt cttcaatctt aaaaccacat
    11521 ttttccaata ctttacttga ccctctattg taggaatata cattagcatg aattttgtta
    11581 aggttgtatt tttcaaaggc aattttgcat atttctttta ctgcatttgt tgctatccct
    11641 ttgccccaat agttcctagc aagccagtaa cctaattcag catttttatt aatagaggta
    11701 tcttcttcaa atgttaggct gatacttcca atagcccttt cttgataaaa aatagcatag
    11761 ctaagttgtt taacatctgg agttgccaaa caaaaactaa caaaatctct agcgttttgc
    11821 tccctataag ggtgggggaa gttgtcacga agattctttg caatttcttt atcattcgca
    11881 aactcaacta aatcggtaat ataagcataa ttccactcta taagttccat ttttcttctc
    11941 cttttatgaa acagctacaa gaaatctttc taagccaata ccacttatca aacagcgttc
    12001 ttttttattt ccaagcattt gataagtcca gtcaacaaat ccaccgtcaa ctatttcaac
    12061 ggtttcattt ccttgtttga ccgataattt gaaattgata cctttataat aattattatc
    12121 aacatcatca ttatgaattg ataactctat attaggaaaa ataagtttga tagtttcaac
    12181 cattctgtcg aaaaatccgt cattatcttt gtatccgttt cttttgcgta gaataataga
    12241 gaaacgaaca ttttctattt cacttaatat atctttataa tagtttaagt gcttttctaa
    12301 caacattttt tcacaagtgt aagaccctgt atctatacca gatgaaacca tacaatataa
    12361 gccaaaatgt gaatataaac ctttacctac aaaattttgt gctcttatta ccctagcagt
    12421 agtagccatg tggataggta tggtattatt ttcttctttt ctttttaatt tatcagcaat
    12481 aattattgcc aacatatttg aggggtctgc aagtgtttct gtccctcgta gagcacttac
    12541 tacattatat tgatctacgg agccaaaaac gctacagcta cctaatggag cagagggaga
    12601 aagcataatc gtcttgatat ccatattgaa tgcttttctc aataattttg actccaaagc
    12661 atgaatttct tctggatcta taccgctagg gatagtaaat ctatttagtt ggtatgactt
    12721 tagaatatca gttgcagtta aagtatttgc ttgtctatca aacacttcta atagaagtga
    12781 gttcaaatct gattttgata gagctgataa cttatcaact atattcttat ccccaatttt
    12841 atttgtaatt ctacttaaaa tatcactcat agttatcctc catactgtta ctttcaaaat
    12901 aattatagaa tggttacaac aactaatcta ggtcatttga ccaaaaatga aaaaacactg
    12961 aatatttact attcaatatt tttttacttt gggtttattt gattaaatct tgaaaatact
    13021 gtaatgcaac cctttcagct tcaacctttc ggacaatagg actttgtgat aattcctctc
    13081 ttaactcaag atacaggttg atagtagcct gcaatgcatt aacacagctt ttatattcat
    13141 ggttagcaac cgtcttttct aatgcttcaa tatattgggg tgcgtccaat tcaatttttc
    13201 taactccacg aggtaattta cctaagagaa gctgtattaa tggggcaagt actgattgtc
    13261 ttaaaaaaga taaaaaatca attgtttcaa atagttctct acgtccaatc ttagtgcaag
    13321 cgtaatgaac ccaaatccaa aatctatctt caatccactg taagtctgga gttgggaagt
    13381 atgcttcttt tttagtataa cacctctcac tttcttttat tagctatcaa tccaacataa
    13441 ttatgcatag gagtattata taatgaaaaa ataaaaaaaa ataggtcact tgacccgaaa
    13501 taaaaaagca ttgaataatt attattcaat acttttttga aattatactt atgttgtagg
    13561 aagattcttt aatacctctc gctgattaat tacactaaat cttttgtttt cataagcaat
    13621 tagatgttct tgtacacatt catttatagc acgattaatg cttctaattg gagcgtttac
    13681 ttcgctagca agctgttttt tggtaagcag atttaattca cttctataat agtgtaagga
    13741 tatacacctc agaagtcgtt ctttaacact taaagatgat aattcttcaa ttaattcagc
    13801 atttacaact aatttactag atatttctcg aatcagaaat tttgtagctt caaaatctcc
    13861 ccttaaccag tttaaaaaat catctctatg aagtttatat acattacaat ctgtgaaagc
    13921 gacaatttca acgggttttt tcccatcatt aaattgttcc atttctccaa aaaaactacc
    13981 acttctataa agatgaatag ttgagatgtt tcctttcaca tttaagatga atgcttctgc
    14041 aataccgtca atcataagaa ttacatattc attttcttgc tcagcaaaaa gaatagtctc
    14101 tttcttttta tacgtttttt taacaagctt ctttttaata tcttcattca taaaatcaat
    14161 taaatgcata ttcatccctc cttaccaaat ctttgattaa attatatcat ctttcaatta
    14221 gttaggtaag atagtaaaaa tatatttaca tagaaactat tccattatta ttttccagtt
    14281 gtcatttttc tgcaaagtaa gctcaaactg agaaagctgt tgacaataga actagtagga
    14341 atatatgagc gaaccatttc agtattcaac tgttgtaggt tttcagtcag ataattcttc
    14401 aaacgttcaa ttcgtttgtc ctgtgaatct tgcgatgctc taaccagttt catagatagc
    14461 gttttacctt gtgccacctg caaaaaagac catgcttcgt caaggtcaac tatcttgaag
    14521 ctagagcggt cagtatgaat aaagtctaat gcaaaggtac taataacaat caacatagca
    14581 acactaagaa gttctattgt tgtatattct tcaaagctag tgttggcatc tggcaatacg
    14641 aggtcagcaa cttgaataat attcagctgt ttctccaagc tgatagactg cttcacattt
    14701 ccgtcaccaa acagtagatg tgcaaaatca tagtctacaa aactttcgat atggtcggct
    14761 atgctgttac tgatagaagt atcttctttt cgtaattcat caatgacaag taataaccct
    14821 cgctgttcac tttgggttac attgcggatt gcttttctaa gcactgggaa cttatcactg
    14881 tcacgactgg agataccagc aaggaatgtt aggatatcaa tcgctaaact ttcagaatca
    14941 tttttacggc tcaaaatgac gtaagggtca agcaatcctt tgttttcatc attactggtt
    15001 aaattgacaa tgtttatctc atgggctatt tctggtagcg tttctttcca tttgccgcgt
    15061 tctgctttag ggtcaactat gaccgcctgt ccgccgaata gtaccgcata gtaaacaagc
    15121 atattgttac tgaataattt accaccaccc aatgaaccga taaacgcaga cgctaacaca
    15181 ttggtaatag aaccctttac ccattgacta gcaagggcag gttttagata gacattcttt
    15241 ccagtgtcca gattgtagcc aagatagata tcctcatgtt cgccaagggt ttgtgtcgct
    15301 ccaaaaccaa gtccagccaa aaaatccgaa gtgacatatt gaatataatc attgatgtaa
    15361 cgtttgctga caggaataaa ttcgccatgc aatcccaaca tatccccaaa gggacgcacg
    15421 agcttgagtg ataagtcgtc gtaaaagtct ttgacttcat cacaacgcct ttttagctcg
    15481 tcaagatcct ttgtagatac acggacaaca tagctaagtt tatacataga ttctttacag
    15541 ttaaaagtct gtttttaaaa aaatgtataa attataaatc aaaatataga tactatcaat
    15601 gacccactag gtgcaaaaag cattgatatg gttaaattat cagacgctaa aagctgggct
    15661 atccgaatga gtgaagatgg gtgtgcttat aaatccattg acaattataa gcgctcactg
    15721 aaagcgtcgt ttttcatggc aattcaagac gactgtatta gaaagaatcc atttaatttt
    15781 cagttaagtg atgttttgga agatacaaca gaaccaaaaa tcgctctaac accagaacag
    15841 gaaatgggct tattatcctt tatggagagc gataagactt attgtaaata ttatgatgat
    15901 gttgttatcc tcttagaaac aggactacgc atttctgaat tatgcggttt gactttagaa
    15961 ttggatatga aaaataaagc cattatggta gaccatcaat tgttgagtga tactaagatt
    16021 ggctattatg tagaagttcc aaagaccaca cagggtaagc gagaaattcc aatgactgaa
    16081 agagcttatc aatcatttga acgtatcgtg gaacggcgta agaacgctaa agcagaacca
    16141 atcattgttg atggttacca gaacttccta tttcttaaac aagatggatt accaaaacaa
    16201 gcaactcatt atcaaagcat gttaagagcg ttagtcaaga agtataataa gacccatgag
    16261 gacaagctac caaagattac gccacatacc tttaggcata cactctgtac aaatatggct
    16321 aacaaaggaa tgagtcctaa tatattgcaa tatattatgg ggcataaaaa cattactatg
    16381 acgcttggct attatgcaca tggttcattt agttcttcga gagctgaaat ggaacggtta
    16441 taatcctagt agtatgacta gagtttacta cttatttact acttttgatg gcattcttat
    16501 acggttaaat gccaacttat gcaaagtatc ttccaaaaag aaaatgccga tactccttgt
    16561 agatacagga tatatcgaca tttaccaact tatgaaaaga taatcaaatt taatataagt
    16621 ttttcaaata aaaacatact aaaaagttga ttatccgcta aaacatagta tctatcagca
    16681 ttttcaagga aacaaacaca tcaatttact actaatttac taccaatgaa tgagccttga
    16741 tacgcaagtt ttcctagata tgcaaagata tggtacggca catcagtatt gaagcaagaa
    16801 aaaaattgaa tatttcatag actatggaac gcctaaattg acgttccttt tctatttcca
    16861 gccgttcatt atggacggct attttattac ccaaaatgaa aggagcattt agaattatga
    16921 aaaagatatt gaaacgattg tgtacgggcg tacttgcctt tgcgactgtc gttactgctt
    16981 taccgactac acttgtccat gcagaaagca agcaatactg gacggaaagt gaagaacgtg
    17041 tcggtatcgt tgaaaaagta atgaatgacg gttcgattgg ttctaccttt aacgaggggc
    17101 atttgaccgt tgagggcgag gacgcttatt gtatcgatat taacacagat tttaagaacg
    17161 gctacaagac cagagctgac gcaagctccc gtatgagtgc cgaccagata agtgatgttg
    17221 ccttatctat cgaatatgtg aagcaataca ccgacaacca caatgggata agcaaaaatc
    17281 acgcctacct tttaagacag cttgttgtgt ggcaaagatt gagtgttcac ttaggctggc
    17341 aatgtgataa cgtgcgagct tcttatgatg aaataccaaa ggcaacacaa gatgaagtgt
    17401 ttgctggtgc aaaagccttt gtgaaagaaa acaaaggacg ctatgaatgc ggcggttata
    17461 tctactctgg cgaggggcag gaattagggc aattctgggc gaagttaaat gtcggaaatg
    17521 ccaaacttca aaagacttcc aataatacca gcattactaa cggtaacgga aattactcta
    17581 tcgctggtgc gacttacggt gtcttttccg ataaggattg caccaaacag attgctaccc
    17641 ttacgactga cgacagcgga aatacagagg ttgcagaagt caaagctact accgtatata
    17701 tcaaagaatt atctgcacca ttggggtata aagttgatac tacggtgtat tccttaaaag
    17761 tcgaagctgg aaagacagcg actttgaaag tatctgatac gccaaaagtt accgatactt
    17821 tgattgagct ttttaaaatt gacatggaaa cacagaaagc caacccacaa gggaacgctt
    17881 ctttagaggg ggcggagttt acatggaaat attatgcagg cttctacaat aaagacaatc
    17941 ttcctgccga agctactcgt acatgggtta caaagacaac cgctgaaaca gacagtaacg
    18001 ggacaaccca ttacatcaca aaattagcag acgcatacaa ggtatctggc gatagcttct
    18061 atatgcagga cggaaaagcg tttttacctc ttggtacact aacggtcgaa gaaacgaaat
    18121 cgccaacggg ctacttacta gacggtgcat atatgcaggc gaatggtagt gaagaacaga
    18181 taaaagcctt ataccttacg cagattaccg aggacggcga ccttgccgta ttaactggaa
    18241 gtaaccagtt ttccgtatca gataaagtca ttcgtggcgg tgtcaaaatt cagaaacgag
    18301 atttagaaac caaagatacc aaaccacaag gaagtgccac tttgaaagat actgcctttg
    18361 acatcatttc cttaaatgat aatgcggtat tggttgaggg caagatatac aagaaaaacg
    18421 aagttgtaaa aacaatccat acggatattg agggtatcgc ttctacttct gctgaccttt
    18481 tgccttacgg gaatttccgt atcgttgaga aacaagctcc aaacggttac ttgacagacg
    18541 gtgcgaagcc gattgatttt acaatcacag aaaacggaaa aatcgtggac ttaacagacg
    18601 aagcccactc tatctacaat cagattaagc gtggggatat tgagggcgtg aaaatcggtg
    18661 caggcacaca caagcgtctt gctgatgttc cctttaggat cacaagcaag acaacgggcg
    18721 aaagtcatgt tgtggtaact gacgataatg ggcaattctc cacttcttca aaatgggctt
    18781 cgcacaagca caataccaac gctggcaaaa caagcgagga cggtgtatgg tttggaactt
    18841 ctgaaccaga cgacagcaaa ggtgcgttgc cttacgatac ttacattatt gaagaattac
    18901 gctctgacag caacaaaggc atggaactta tcccaccatt tgaaatcgtg gtatcaagaa
    18961 ataatcttgt gattgattta gggactttga ctgatgaata cgaaaaggaa atcactatcc
    19021 ataccacagc aaccagtaaa gacggcgaaa agacaatcct tgcaggaaaa gaggtaacaa
    19081 tcgttgatac tgtcaaatta gacggactta caaaaggcac taagtatcag ttaaaaggct
    19141 ggcagatgtt aaaggaagaa aacgccgaga ttatcattga cgggaaacgt gtagaaagcg
    19201 attatacctt tgttgctgat gatgaagaaa tgaaagtgga aatttcttat acattcaatg
    19261 cgtccgagct tggcggtaaa aatctggtaa cattcgagga attatacgac ttaagcaatc
    19321 cagacgaacc catgaaagtt gcggagcata aagatattga ggacgacggg cagacggtac
    19381 ttattacaga acgtatcatc aaaatacata caactgctac cgataaggac ggtaacaaag
    19441 agcttgaagc aggaaaagac gtaacaatca ttgatactgt aaccttagag ggcttggaag
    19501 ttggtacaaa gtataaactt gtgggctggc agatgttgaa agaagaaaat gcagagcttc
    19561 ttataaatgg aaaacgtgtg gaaagtgatt atacgtttat cgctgacagc gaaactatga
    19621 aagtggaagt tacctttacc tttgacgcta cttctcttga cggcaaacag cttgtaactt
    19681 ttgaagaatt gtatgattta agcaatccaa acgagccgaa aaaagttact gagcataagg
    19741 acattgagga cgagggacag acgattactt ttaaggaaaa accagaagtc ccagagaaac
    19801 ccgaaacgcc aacaacacca gaaaaaccga gcagaccaac agacagtcct aaaacgggcg
    19861 acaacacaaa tgtcatggca tttctggtaa tgttgcttac atctgctggt ggactggctg
    19921 gaacatatct ttacaaacgc cgtaaaatga agaaatcata aggcggtaac ggtatgagaa
    19981 aagaaaaatc acgctctctg ccgaagctgt caaacggcag acgtttactt attctggctt
    20041 gtctgccaac aacctgcaag cacacggata gtcaagggtg cgaagcattg atttaccctt
    20101 tactatctgg gggattactg gtatttccct aattcccaga ataagaaagc acaatcaaca
    20161 aacttaaaaa agaagaaaga aacgaggtaa aacactatgg aattaaaata tgtagtgccg
    20221 aacatggcac agacttttgg aaacttggaa tttgctggcg aaaacaagat tgaacagcaa
    20281 cgcattaacg gacgctttac gcccgtatca agaagctaca acttgtactc tgacgtacaa
    20341 agggctgatg atattatcgt aaccattcca gcgaaagcag gcgaaaagca tttcaaacca
    20401 gaacagcgag taaaacttat caatccaaag attaccgcag acggtaaagc ggtcaaagga
    20461 cgagggttca caaactatat cttaacggct gatgatatgt tgccagccga ggaaagcaag
    20521 taagggggta aatgattatg agattagcaa acggtattgt attagacaag gaaaaaactt
    20581 tcggtgtatt gaagttctct gccctcagac atgaggtgcg agcagaaaat gacgacggtt
    20641 caacaagtga agaaatcaca cgaagaacct atgatttgaa atgtagtgtg caggaacgta
    20701 tgatacaagt atcaattcct gctgatattc ccgtcaagga ctatccgtat aatgcagagg
    20761 tagagcttgt caatcccgta gccgatacgg tagcaaacgc cagctatcga ggtgtcaatg
    20821 tggactggta tatcaaggct gacgatatta tcttgaagaa taaaggcggt caagtaggca
    20881 atccacagaa ccatgcaccg caggggcaac caaagaaata agctggcaaa ctagattttc
    20941 attgtagcgg atataagcaa ctgttataat atgtgtatga agaaacggat aaattgaaat
    21001 ttgtggagga aaaagataat ggcaaaagaa aaaggttcat tcaaagaatt tctatcggca
    21061 atcgcgccag aacaccaagc gtttgtcgag aaacttaata ccaaacttat tgagcagggc
    21121 tgtgaccttg taataaagga agcaaaaagc ggttacaccg cttcatacca actagagaag
    21181 aaaacagtaa tgaattgggt gtttcgcaag acaggtgttt ttgcacggat ttacggagac
    21241 aacgctggaa agtatgagga tataatcgct tcgcttcctg ctgatatgca aaagaaaatg
    21301 actacttctc gtgactgcaa gagactgatt gaccctaatg cctgtagtga tacctgtgta
    21361 aaaggttttg tttatgcctt gaatggcgac acacataaaa aatgccgtaa tgacggaatg
    21421 ctctttgtac tgacaaatga aacagcggag catatagctg ggctggtctg tgcagaggtt
    21481 aatgtgcgta agtcagcttt ataacgacag aaaagttaag aacaataaat cccagtttga
    21541 caacttcata tccatacaca caaagcagtt agtcttagga ttgactgctt ttttcgtacc
    21601 cagaaaggag tgattgattt atgaaaatga ttttaaacaa aggacaccgt atcagagcca
    21661 gcgacaagaa ccttgtctac cacttttcca tagggacgct tctgtttgta ttcattgcgg
    21721 ttatcctgct actgaatatg aaacagctca tgcgtaccga ttgggagcat ttcagcttat
    21781 tagacaacgg tttgacgctt tccacttaca acttcataac cctactgata gcgactggtg
    21841 tttgtgcatt ggtcgctttt ctgtattacc gcttctgtta tgacagtttc aagaagctcc
    21901 tgcaccgtca aaagctggca cgaatgatac tggaaaacaa gtggtatgaa gcagacaccg
    21961 tacaagacag cggttttttt actgacctgc aaagcagatc aagggaaaaa atcatctggt
    22021 ttccaaagat ttattatcaa atggaaaaag gactgcttca tatccgctgt gaaattacat
    22081 taggaaaata tcaagaccag ctcttacggt tagaggataa attggaaagt ggcttgtatt
    22141 gtgagctgac cgacaagacg ttacatgacg gttatatcga atacaccatg ctttatgata
    22201 tgatagcgaa ccgcattacc attgatgaag tgaaagcaga aaacggctgt cttaggctga
    22261 tgaaaaatct tgtatgggaa tatgacgcac tccctcacgc tctgattgct ggtgggactg
    22321 gtggcggtaa aacgtatttt ctgctgacgc ttattgaagc tctgttacat acgaacgctg
    22381 ttctttacat attagacccg aagaacgctg accttgcaga tttaggtacg gtaatgggaa
    22441 atgtgtatca caccaaagaa gaaatgattg attgtgtcaa tgccttttat gagggcatgg
    22501 tacaaagaag cgaagaaatg aagcgacacc cagactataa aacgggcgaa aactatgcct
    22561 atcttggttt accgccctgc tttcttatct ttgatgaata tgtggcgttt tttgaaatgc
    22621 tggggacaaa agaaagtgtg agcctactta gccagctaaa gaaaatcgtc atgttaggac
    22681 gacaagcagg atatttcctt attgttgcct gccagcgtcc agacgcaaaa tatttctcgg
    22741 acggtatcag agataatttt aatttccgtg ttggcttggg gcgtatcagc gaattaggtt
    22801 acggtatgct gttcagttct gatgtgaaaa aacagttttt ccagaagcgt atcaagggac
    22861 gtggctattg tgatgtggga acaagcgtca taagtgaatt ttacacaccc ttagttccaa
    22921 aaggacatga ttttttgcag accataggct cacttgcata cgcaaggcag gacgggacgg
    22981 cgacgtgcga agcgaaaggc gacggcacgg actagccgtt gctggtgtgg cgttagccac
    23041 gccagcagat acaagcccct cattatctaa cagaggggca caattacggc ggaaacttcc
    23101 acaaaaacct ttgcaagacc gcatgaaata agggtttttc gtacatttcg ccttttgtga
    23161 taaagccttg aaattatcac aatatcacaa aattatcacg attgggggta tgacaactga
    23221 atgaaactga ttttttaacc gccttgaaag aaaaacgctg tgcatacggc gtatcgcaga
    23281 caaggcttgc aatcatggca aatatcagcc gtgaacattt gagccgtatc gaagcaggaa
    23341 aggtaacgct gaccgaggac atgaaacata cgcttttaga agcggtggaa aaattcaatc
    23401 ccaacaatcc catgttcctg ctgtttgatt atgtgcgtat ccgttttccc acaatggata
    23461 taaagcatat catcaaggat atattgaagc tcaacatcgg ctatatgctt cacgaagatt
    23521 acggacatta caaatacacc gagcattacc atatcggaga tgtgtttgtg tatgtatcgc
    23581 aggacgaaga aaaaggtgtc ctgctggaac tgaaaggcaa aggctgtcgc cagtttgaaa
    23641 gctatctgct ggcacaagag cgaagttggt atgacttctt cacggacgca ttggttgagg
    23701 gcggtgtaat gaagcgtctt gatttggcaa tcaatgaccg aacgggctta cttgatattc
    23761 cagagctgat acgaaaatgc gagaatgagg aatgtatttc taaattccgt tctttcaaaa
    23821 gctatggttc tggagagctg gtaaagcata atgaaacgga taaatacggt atgggacaca
    23881 cgctgtatat tggttcattt tcaagtgaag tgtacttctg ctgttatgaa aagaattacg
    23941 aacagtacgc caaacttggc ataccgattg aggaagtccc cataaaaaat agatttgaga
    24001 tacgtttgaa aaatgaacgt gcctattatg ctgttgtgga actgctgaca cattatgacg
    24061 cagaactgac agcattttct attatcaatc aatatatccg cttcgctgat aaagaaccag
    24121 acaagcgaaa aagcgactgg aaaaccaatg tacgctgggc ttggttcatt ggcgaggggc
    24181 gaccgcccgt aaagctgaca acacagccag aaccttacac gctggagcgt acactcaact
    24241 ggctacaaag gcaggttgca ccgaccttga agatgttgaa gcagatagac aaggacaatc
    24301 agacggagta tctggaaacg attgaaaagc aggcaaaact gaccgaacga cactaccaga
    24361 taatcaagca acagacagcc acggttgaag aaatggttgc caacgtaaag gagtgatgaa
    24421 aaaatgtggg aattattgaa agacttattg ctggtatcat ttggaatggg tatcggtgta
    24481 gtcttgatgt gcattttaac tgtcggcaaa gaagctgaca gatgtatgga agaaatcaaa
    24541 gaaagagagg acaattaaat gaatttcgga caaaatttat gtgcgactcg ttccttagtg
    24601 aaaagctaag gcactaataa aagaacgaaa aagtttggtt attaggagaa ctgataatct
    24661 gacaggtgga atgatgaagt aacgctctga aacgcctgcc ctgagcctcc gacaagcatt
    24721 gtatgaggaa actcagaatg tttgaagctc ggtaaagacg gctgaaagat accgtaatag
    24781 ttgatgaaca tatatcttct atcgaaaact atccagaggc gggtagcgta tccaacaggt
    24841 cgggggtcta taaaatatct atggttagaa tgttctataa ggaactgacg aaacagcgaa
    24901 tgtacgggtc taaatttgaa ctttgtagaa atgcaaaggt acgagaaatc gtagctggtg
    24961 tggcaaagta agaatatagg ttatgaaatg ccatataacg ttacaggcgg tatcaagcca
    25021 gcaggcttat agctagtgac ctaaggatat atatgtatag ataagattat cggaacgagg
    25081 aaaggtattg ggttctcatc aagagaataa actgacgaag aacaataagc agttgaacta
    25141 atgctgaaaa gcagagggcg aacctaagaa attcttgtaa tgagaatgga ggaatgccct
    25201 caagtcgtgt atatattaga aaagtattat gttgtttcaa taaggattac gagtaagata
    25261 aaaaagctca ctcaatttca agcaaaggag tgaagcctta tgcctaaaaa taaaaatgaa
    25321 acattgtgtg tagaagattt aagacacgct gaatattacg aaatgcaaaa cacctttgat
    25381 gatttatatg ccaaaagtaa gaatggagat attttcactc atcttatgga tattatccta
    25441 tcgagagaaa atatattact ggcatatcga aatatcaaag caaatgcagg cagtaaaaca
    25501 gcaggaacag acggtacaat aattaaagat attggcaaat tgccagcaga aacagttgtc
    25561 aaaaaagtaa gatacattgt tgcaggcagt ccacatgggt atcgacctaa gccagtgagg
    25621 cgaaaagaaa taccaaaacc aaatggtaaa accagacctc taggtattcc ttgtatgtgg
    25681 gacaggctta tacaacaatg tataaaacaa gtattagaac caatctgcga agctaaattt
    25741 agtgaaaaca gctatggttt cagacctaat aggtcagttg aaaatgcaat aaaagcaacc
    25801 tataaccgcc tacaaatcag ccagctacat tatgtgattg aatttgatat taagggtttc
    25861 tttgacaacg tgaaccattc caagctgata aaacagatat gggctatggg aatacgggac
    25921 aagcacttga tattcatttt aaaaaggata ttaaaagctc caatcaaaat gactaacggt
    25981 acaataacat atccagagaa aggaactccg caagggggaa taatatcccc actactagca
    26041 aacattgttc ttaatgagtt agaccattgg gtagaaagcc aatggcagga aaatcctgtt
    26101 acaaaaaact atgtggttca tatcaataaa agcggtagcc catgtaaaag caacgcttac
    26161 aaagaaatga agaaaacaaa gttaaaggaa atgtatatgg ttcgttatgc agatgatttt
    26221 agagttttct gccgctacaa ggaaagtgct gaaaaagcca aaatagcaat cactcaatgg
    26281 attgaacaac gattaaaatt ggaagtatca caagaaaaaa caagaattgt aaatgttaga
    26341 aaaagatact ccgactttct tggtttcaag attaaaatga taccgagaag aaagaagtta
    26401 gttgttaaat cacatataag tgataaacaa ttcaaaaacc aaaaagacaa gctggtatca
    26461 caggcaaaga aaattgcttc tccctcaaaa gggaaaacgg aattagaaga aacaagactt
    26521 tataattcaa tggtattagg tatgcaaaat tattatcaga ttgcaacgaa tgtaaatctg
    26581 gattgtatga aactgaatag agctgtaatg gctatcttca ctaacagatt aggtacaaac
    26641 aaaaggggca gattgagaaa aacaggcaga caacttacga caaaggaggc aacaagatat
    26701 ggtaaatcta aaatgctccg gtatgttgct ggcgagggcg aacctatttt ccctgttgga
    26761 tatatccaac acagaaaacc aatggcaaag gtgtatttag cgaattgcta cactctagag
    26821 ggaagaaaat ttatccacac caatctaagt gttgataaat tactgatgta ccagttaatg
    26881 aaattatctt tggaaaatcg cacaatagaa tacgcagaca atcgaatttc gctattttct
    26941 gctcaacatg gaaaatgtgc tataactctg gaagaatttc aacaagctaa tgaaatacat
    27001 tgtcatcata ttgtaccaac tggagctggt ggaaatgacg attataaaaa tcatattttg
    27061 gtaaaagagg cggtacaccg tctaatacac gctaaaacag aagaaacaat acaaaaatat
    27121 atagctctat tgaagctaaa taaagtccag ctcataagac taaataaata tagagtatta
    27181 gctggcaatc aagaattaaa cttgatatag gtctactaca taaatacttt tcaatataaa
    27241 cacgatggaa cgcggagtgc gttgaaagtc gcacgctccg tgtgaatggg gggaaaagtt
    27301 tgagatattt ataatatcag gaacttacct atccatatac acatggtttt taagcaacgc
    27361 ccagagctta gtgcttatgg cgattgtggt tattggtatc tacttagggt tcaaacgtga
    27421 gttttctaaa cttatcggct tcttggtagt tgcccttgtt gccgttggat tggtattcaa
    27481 tgctggcggt gtgaaagacg tactgttaga gctgttcaat aagattatcg gtgcgtaaaa
    27541 ttttattgtg gtgctgatat gagaatgata taatgtggat atgaagaaag cgacaaattt
    27601 gaagttgagg aggacaacta ttatggataa agtagttatt tatatacatg gtaaaggtgg
    27661 aaatgcagag gagtcttcac attacaaacc tctttttagt gattgtgatg tggttggact
    27721 caactatact gcacaatatc catgggaagc aaaagaagaa tttccatcac tttttgattt
    27781 gatttgcaaa cattacaaat ctgttcaaat agttgcaaat agcataggtg cctatttcgc
    27841 tatgaacgcc ttatcagata agcaaataga aaaagcatat tttatttcac ctattgtgaa
    27901 tatggaaaaa ctgattgcag atatgatgta ttgggcaaat gttacagaag atgaacttcg
    27961 agataaaaaa gaaattaaaa caacttttgg agaaacactc tcatgggaat atctctgtta
    28021 tgtaaaagaa aatccaacta catggaaaat tccaacaaat attttatttg gtgagaaaga
    28081 taacttgact tcttatgcaa ctatatctga atttacaaaa caaactaatg caacacttac
    28141 tgttatgaga aacggagagc attggtttca cacggaagaa caaatgaaat ttcttgatga
    28201 gtggataaac aagtctgcca gataaatccc agtttgacaa cttcatatcc atacacacaa
    28261 agcagttagt cttaggattg actgcttttt tcttacctaa atttcagatt ggagtgatgt
    28321 aatgaacgat atttttagag atatgcaggc aaaagttggt tgtgaaaaca tttcagacct
    28381 gccctcttac aagcgtaagg tgtggcatga aatgaagcaa ctgaaccttg ccgactatga
    28441 agaaaaacag ttagaagatt tttcaaaata tgtgtttggt atgtcatgcc agactttaaa
    28501 agatgtgatg aaacaacaga aaggacgtga ggaacaatgc aggaaacaag ggtactggtg
    28561 gaaacgaaaa gaacaactgg cgaagaaaca acatcatact ggttcgacct gccgatagag
    28621 gttgccgagt ttgaagaaaa gttaggtgtc agtacagaaa gtcaagatta ccgcattatt
    28681 gaaaaattgt tgccttatgc tgatgaagtc catgagcata caagcgtgta ccagctcaat
    28741 gaattagatt atatgtatcg ccagctttca agcgatatgc aggaagaata tacaatgctt
    28801 catacggtgt atgaaaattt agaagcactt tatatttgca ggaatgtaat tacgctttat
    28861 cccgactgta aaagcatgat tgatgttgca aggcaaaagc tgatgaatga ccctacattc
    28921 aaacatttat ccgaggactg tcaagaatac tattttgact ttgaaaccta tgcttcacac
    28981 ttgcaggaac acgggaaatt tttagtaacg gaacacggta tctttgaatt gccagagtaa
    29041 gtatgattga tgatatggca gtttatatcg ctaatcttgg caagtataat gagggctatt
    29101 tagtcggtgc ttggtttact ttccctattg acgaggaaga tgtgaaagaa aaaatcggct
    29161 tgaatgaaca gtatgaagaa tacgcaatcc atgataccga taacttcccg attgagattg
    29221 gggagtatgt ttccattgat gaactcaatg aaatgtatga aatgatagag gaacttcccg
    29281 actatatcgt ggattgtttg gacgaattta ttggtcatta tgggacgctg gaggaagtcg
    29341 tggaacacaa ggacgatatt tactattatc ccgactgtga aaccatgaca gatgttgcct
    29401 attactacat agatgaattg caggcacttg gcgatattcc ccctagcttg cagaactata
    29461 ttgactatga agcatacggg cgagatttgg atatgggcgg ttgcttcatt gcaacaagcc
    29521 gaggaatgtg cgagatacca tattaacgct ggaagatcag cgacaagcat tgttgctact
    29581 gacggcgtat ttctctttaa gggacagtct gaaacagggc tgtccttttc catttagaaa
    29641 cttatgagag gagttgaaag aacttgaaaa agattagaag ctacacgggt atctggaatg
    29701 tagaaaaagt cttgtatgca atcaatgact ttaacttgcc ctttcccgtt acctttacac
    29761 agattacatg gtttgtgatt acagaattta tcatcattct gtttggggat atgcccccac
    29821 tttccatgat tgagggagca tttttaaaat acttcggtat tcctgctttc ctcacttggt
    29881 ttatgtcgca gaaaaccttt gacggaaaga agccatacag ctttttgaaa tcacagataa
    29941 cctatgcgtt gcgaccgaaa atcacttatg caggaaaagc cgtaaaactg caaaaagaaa
    30001 tattcaacga aacaatcacg gcagtaagga gtgtgagtta tgttcccgat taaatatatt
    30061 gacaacaacc ttgtctggaa caaggacaat gaggtgttcg cctactatga gctgataccg
    30121 tataactatt ctttcttatc cgcagaacag aaatttatcg tgcatgacag tttccgacag
    30181 ctaatcgcac agtcccgtga gggtaaaatt catgctttgc agattgccac ggaaagctct
    30241 ataagaagta tgcaggagca gtcaaaaaga ttggttacgg ggaaattaaa ggaagtcgct
    30301 taccagaaga tagacgaaca gaccgaagcg ttagtatcaa tgattgggga caatcaagtg
    30361 gactaccgct tttttcttgg ctttaaactc atggttacgg aagaacagct caatctgaag
    30421 aaccttaaaa aatcggcgtg gctgacgttc aaagagtttc tccatgaagt gaaccacacg
    30481 ctgatgaacg actttgtttc catgccgaat gatgaaatca accgttacat gaaaatggaa
    30541 aagttactgg aaaataaaat ctcccgtcgc tttaaggttc gccgtctgga aatcaatgac
    30601 tttgggtatc ttatggaaca tctttacggc agggacggta tcgcctatga agattatgag
    30661 caccagctac caaagaaaaa gtataagaaa gaaacactta tcaagtatta cgaccttatc
    30721 cgcccgacaa ggtgtgtgat tgaggaaagc cagcgatatt tacgattgga acatgaggac
    30781 aaggaaagtt atgtgtccta ttttaccgtc aatgcgattg taggcgagct tgattttccg
    30841 tctagtgaaa tcttctattt ccagcaacag caatttacct ttcccgttga tacgagcatg
    30901 aatgtagaaa tcgtggaaaa ccgaaaagca ttaacgaccg tccgcaacaa gaaaaaggaa
    30961 ctgaaagacc ttgataatca cgcttatcaa gctggaagtg aaaccagctc aaatgtagta
    31021 gacgctttag acagcgtgga cgagctggaa accgatttag accagacaaa agaaagtatg
    31081 tataagctct cttatgttat ccgtgtgtct gctgatgatt tggacgaact gaaacgccgt
    31141 tgtgatgaag tcaaagattt ttatgacgat ttgaatgtga agttagtacg tcctgctggg
    31201 gatatgctgg ggcttcattc tgaatttcta ccagccagca agcgatatat caatgattat
    31261 gtgcagtatg taaaatcaga cttcttagct gggcttggtt ttggtgcgac acagcagtta
    31321 ggggaaaaca cgggtatcta tatgggctat tccgttgata cgggaagaaa tgtatatctg
    31381 caaccgtccc ttgccagtca aggcgtaaaa ggtacagtta caaatgccct tgcttctgct
    31441 tttgttggtt cgcttggcgg tgggaagtca ttctgtaata acctgcttgt gtactattct
    31501 gttttgtttg gcggtcaagc ggttatctta gacccaaaat cagagcgtgg aaactggaaa
    31561 gaaacacttc cagaaatcgc ccatgaaatc aatatcgtaa acctaacaag cgacaaggac
    31621 aacgcagggc ttcttgaccc gtttgtgatt atgaagaatg tgaaagacgc tgaaagtctg
    31681 gcaatcgaca tactaacctt tttaactggt atttcctcta gggacggcga aaaattcccc
    31741 gtacttcgta aggcggtgcg ttctgttacc cagagtgaaa caaggggctt gcttcatgtg
    31801 atagacgagt tacgctgtga agataccgca atttcacgca atatcgcaga ccatatcgac
    31861 agctttacag actatgattt tgcacatctt ctgttttcag acggaacggt ggaaaatgct
    31921 atcagtttag ataatcagct caatatcatt caagtagccg accttgtact gccagataag
    31981 gacacgactt ttgaggaata cacgaccatt gaattactat cggtttccat gctgattgtg
    32041 attagtacct ttgccttaga ttttatccac agcgaccgct ctatcttcaa aatcgtagac
    32101 cttgatgaag cgtgggcgtt cttaaatgtg gcacaaggcg aaattttatc taacaagctg
    32161 gtgcgtgctg gacgagctat gcaggcaggt gtgtatttcg ttacgcaatc ttctggggac
    32221 gtgtcaaagg aaagtctgaa aaacaatatc ggcttaaaat tcgctttccg ttctactgac
    32281 atcaacgaga taaaacagac tttagaattt ttcggtatcg acaaagagga cgaaaacaat
    32341 cagaagcgac ttcgtgattt ggagaacgga caatgcttgt tgcaggactt atacgggcgt
    32401 gtcggcgtgg tacagataca ccccgtcttt gaagaactgc tacacgcctt tgataccaga
    32461 ccgcctatac agagaaatga ggtggagtga tgaaagaaag gatacagagt gcgttcacaa
    32521 aaaagaagat tttccacttt ctcaaaatgg ctctgttcgt ggtggcactc tcccttatcc
    32581 tgctttcact tctggggacg gtggctcatg cgacggggct tgtggacgat acgataaacg
    32641 cagaaaatct atattccaaa tatccgcttt ccaactatca gcttgacttc tatgtggata
    32701 atagctgggc ttggttgccg tggaactggc tggacgggat tggaaaatca gtacaatatg
    32761 gtttgtactg tatcactaat tttgtctgga ctatcagttt atatttaagt aatgccacgg
    32821 gctatgtagt gcaggaagcc tacaagctgg acttcatcaa tgatatggca gacagtatcg
    32881 ggcagagcat acagaccctt gcaggcgtta cctcaagcgg tttttcaacc tctggcttct
    32941 atgtcggttt cctgcttctc attatcttag tggtgggact atatgttgcc tatacgggac
    33001 tgattaaaag agaaaccagc aaggcacttc acgccgttat caactttgtt gtggtgttta
    33061 ttttgtcggc ttcatttatt gcctatgctc ccgactacat caaaaagata aatgaatttt
    33121 caagcgacat cagtaccgct tctttggact tgggaacaaa aatcatgctc ccaaactctg
    33181 acagcgaggg caaggacagc gtggacttga taagggacag cctattctct attcaagtgg
    33241 aacagccgtg gctactctta cagtttggga acagcaacgc agaggaaatc gggacagacc
    33301 gtgtagaagc tcttgtatcg gcaagcccag agaacgagga cggcaaaacc agagaggaag
    33361 tcgtgaaaac agaaatcgaa agtaacgaca acaacaatct gacgataccg caagtcgtaa
    33421 accgtttagg tatggtgttc ttcctcttgt tcttcaactt aggtatcacg atatttgtat
    33481 tcttgcttac gggtatgatg ttgttcagcc agatactttt tattatcttc gccatgtttt
    33541 tacctatcag ctttttacta tccatgatac cgagctatga aagcatggca aagcaggcaa
    33601 tcgtgagggt atttaatacc ataatgacaa gggcaggaat tacgctcatt gtaacggtgg
    33661 catttagcat ttccagtatg ttttataaca tttccacaga ctacccgttc tttatggtgg
    33721 cgttcttgca gatagtgtgt ttcgctggta tctacatgaa gttaggcgat ttaatgagta
    33781 tgttctcttt gaacgctaac gacagtcaaa gcatgggacg aagaattttc cgcagaccgt
    33841 atctttatat ggcacatagg gctaggcgta tggaaagacg gcttgctggt gctgttaccg
    33901 ctggcggtgt tgctggtgcg gtggctggca gtacggtttc taatgcaagg acaacaagga
    33961 ataattcagc aaaagacaac cgagggaata catcttccag catgggacaa cgtgctggtt
    34021 caaaggtggg tgctgtctta gatacgaaga ataaggtcaa agacaaggca aacgctatga
    34081 aagaaacggt caaggatata ccgacacaga ccgcttatgc cgtccattcc gcaaaggaaa
    34141 aagcaaaaac aagtgtatcg gacttcaagc gtggcatggt gcaggaacaa caatccagac
    34201 agtcgggacg cttggaaaag caggaacagc ataagaagaa tatcgctgac aagcgtatgg
    34261 aacttcaaaa agcacaagaa gcaaggcagg cacgaaagac ggacggatca gcgacaacgg
    34321 gagctacccg tccccatgag cgaccagtca cagcttcagc cattccaaaa acaaatgttg
    34381 agaaaacaca ggaagtcaaa cgtcctgcta caacttcaac ttccaaagca ggcgagccag
    34441 tcaaaacaaa tagtgtgaaa gaacgtccct tatcttcggg tacatctgac ggaaaaattc
    34501 cacaatcggc acagacagta catagtaaaa atgtagaaaa agtggtatcg catgaaacac
    34561 accagaatta caaagcagaa cgtaccacaa aggcacaaac agttgaacaa gcaaagcgta
    34621 caacagaaca tactgaaaag aaccgtaacc ttgtaacgaa gaaaggacag aagaaaaaat
    34681 gaaattgaaa catatcgcta tcattggcag tctgtttcct atcctctttt ccatagtcct
    34741 tttctttggg gtactgatga gtgcggacag cgacgacgag aacagcaact tttcttcggg
    34801 cattacgggt atgaatttat ccgcagaagt cttgaaacat cagcctatgg tggaaaagta
    34861 cgctaaagaa tacggtattt ctgagtatgt gaatgtgtta ctggctatca ttcaagtaga
    34921 aagtggcggt acggcaaaag atgtcatgca gagttcagaa agtcttggtt taccgcccaa
    34981 ctcgttagat acagaaagct caatcaagca ggggtgtaaa tattttgcgt ccctgctttc
    35041 ttcctgcaaa aatcaaggaa taaatgattt gaatgtggcg atacagtcct ataactatgg
    35101 cggtggctat gtgggatatg tggcaggaaa aggaaagaaa catactttta accttgcaga
    35161 gagctttgcc cgtgagaaat ctggcggtaa aaaagtaacc tacaccaacc cgatagccgt
    35221 tgcgaaaaat gggggctggc ggtatggcta tggaaatatg ttttatgtgg aattagtcaa
    35281 tcagtattta actgttccgc aggtatcggg agaactggca caaaaggtaa tgaatgaagc
    35341 gttgaaatat caaggctgga aatatgtgta tggcggtagc aatcccaaca cttcctttga
    35401 ctgttcggga ctgacgcaat ggtgttatgg aaaagctggt atctctttac cgagaacggc
    35461 acaaatgcaa tatgacgcaa cccagcatat ccctttatcg caggcaaaag ctggggagtt
    35521 ggtctttttc cattc
//
LOCUS       DAEPIN010000044        34552 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.044, whole genome shotgun sequence.
ACCESSION   DAEPIN010000044 DAEPIN010000000
VERSION     DAEPIN010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 34552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34552
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.044"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            745..2415
                     /locus_tag="KR250_002276"
     CDS             745..2415
                     /locus_tag="KR250_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF and EAL domain-containing protein"
                     /protein_id="HBH0068207.1"
                     /translation="MREESNSLSLDHIEIFFELLSQSTEDYIFFWDINRNKFKISSAI
                     FDEFNLSKEIESDLVNCWSKIVYPDDVQIWKDDIQELLHGKKGEHNLEYRLINKYKEI
                     VWISCRGKVYVSDDPKTIFLVGRIKNIGEKNKFDSITGTWNREQFEHKMNYLIKEKIY
                     KNGAMFIMDIDNFKNINEKYGHSYGDKVLRAIATEVLEYLPKDVRLYRLDGDEFAFFY
                     PMCTKETIEKIYEKIQMYTNTQHEIESSKYYCTVTAGVAMYPEDGDNYLDLFKHADIA
                     LDIAKISGKNRIKFFSQELYENKLKVISMQQKLRECVENNFNDFELFFQPQVNAVTKE
                     VIGAEVLLRWHSSTYGEVSPVEFIPILEQSNLIIPVGKWIIKEAVKQCKEWHKINPDF
                     KISVNVSYIQLKEDFFRDFIVECLVEYQLRPEFLILELTENCWIPDINLLNDKFISLK
                     GIGVYIAIDDFGTGYSSLNYLKELSVNIIKIERSFVKNITYNSYEYTFLEYIIKLAHI
                     INLKVCVEGIESYEEYDIVKSLGVDIIQGFLFGRPVSASEFYKFQLSK"
     gene            complement(2537..3271)
                     /gene="pflA"
                     /locus_tag="KR250_002277"
     CDS             complement(2537..3271)
                     /gene="pflA"
                     /locus_tag="KR250_002277"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HBH0068208.1"
                     /translation="MIKGKIHSIETFGTVDGPGIRYILFFQGCPLRCKYCHNRDTWDI
                     KSGKEYTVDEIITDALKYTSFMKFSGGGITASGGESTLQPEFLSELFKKAKENDIHTC
                     LDTSGFVDIETIDPVLDNTDLVLLDLKHMVEEKSIDLTGVGMDKALKLARHLESRNIP
                     VWIRHVLVPGITDDIDNLEKLGQFVATLKNVERFELLPYHSMGIHKWESLGIDYELKD
                     VPDATKEDVQKASEIISKFGVKVYNS"
     gene            complement(3431..5662)
                     /gene="pflB"
                     /locus_tag="KR250_002278"
     CDS             complement(3431..5662)
                     /gene="pflB"
                     /locus_tag="KR250_002278"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HBH0068209.1"
                     /translation="MNAWQGFKTGRWTKEINVREFIQLNYSPYEGNDSFLAGATENTK
                     KLWDEAMVLFKKERENGGTLDVDTKTVSGIAAYAPGYLDKELETIVGLQTDAPLKRAV
                     MPYGGIKMVENSCEAFGYELDPEIKDIFTKYRKTHNQGVFDVYTPEMRAARKSGIITG
                     LPDAYGRGRIIGDYRRVALYGVDALIEDKNEQKKSLEVSCMDEEVIRLREEITEQISA
                     LNELKKMAESYGFDISKPATNSKEAVQWLYFGYLGAVKDQNGAAMSLGRTSTFLDIYF
                     ERDLKAGIVTEEELQEYMDHFVMKLRMVKFLRTPDYNNLFSGDPTWVTECIGGMGIDG
                     RTLVTKNSFRMLNTLYTLGPSPEPNLTVLWSTKLPQGFKDFCSKVSIDTSSVQYENDD
                     LMRAYWGDDYGIACCVSAMRIGKQMQFFGARVNLAKTLLYAINGGVDEKSGVQVGPRF
                     EPITSEYLDYDEVMSKFEPFTDWLATLYVNTLNVIHYMHDKYSYEALEMALHDRDIFR
                     TMACGMAGLSVCADSLSAIKHAKVKTIRNEQGIAVDFEIEGDYPKYGNNDDRVDSIAV
                     ELVESFMNKIRKNKTYRNSYPTQSILTITSNVVYGKKTGNTPDGRRAGAPFAPGANPM
                     HGRDTNGALASLSSVAKLPYEHAQDGISNTFSIVPAALGKDMTERINNLSAMMDGYFA
                     QNAHHLNVNVFDRATLEDAMEHPEEYPQLTIRVSGYAVNFIKLTKEQQLDVINRTFHG
                     KMA"
     gene            5955..7016
                     /locus_tag="KR250_002279"
     CDS             5955..7016
                     /locus_tag="KR250_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HBH0068210.1"
                     /translation="MEKRKSIHLHKLNIYLIGGGDRLFITVVLFLVGFLLITKGADIF
                     INCTVEIGKKTNISEIILGATIVSFATTLPEFTVSLLASIDGHTTMSLGNAVGSIICN
                     TGLALGLVVFISPFNVDKKMFFSKSLLLIVSVIVLILLSLDGVITRGDSLLLIIILIF
                     YMVNNYRSVVGKSDTKNRNIKNNTIKDISTKKGKNYRGFSVLEIVKILLLFATGLIMM
                     IIGSQILIESGVIIASFLNIPQGIVSLTIIALGTSLPEIVSSITAIRKNHHEISVGNI
                     LGANILNIVSVIAVSAIPNNIPILSQNRQLDIPFMILLLLIVIIPTLKSNRLSRIQGI
                     LMLFTYFLYISILYFMYII"
     gene            7107..8720
                     /locus_tag="KR250_002280"
     CDS             7107..8720
                     /locus_tag="KR250_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBH0068211.1"
                     /translation="MNITKFEDLPISEGIKKAIAEMGFEEPSPIQAQSIPAILSGKDV
                     IGQAQTGTGKTAAFSIPILETIDPNNRSLQAVVLCPTRELAIQVSTEIRKLAKYSHGI
                     KTLPIYGGQPIDRQIKSLKSGVQVVIGTPGRTIDHINRKTLKMDNVKMIILDEADEML
                     DMGFREDIEMILSKIPEERQTTFFSATMPRGILELTKRYQKDPEHIKVVRKELTVSNT
                     KQYYIETRSSNKLEVLCRLVDVYDPKLSVVFCNTKRKADELVGDLQARGYFADALHGD
                     LKQTQRDIVMDKFRNGTIDILVATDVAARGIDVDDVECVFNYDLPQDEEYYVHRIGRT
                     GRAGREGMSFTFVFGKEMRKMKDIERYTKSKLIKHNIPTITDVEEKKVGTFFAQVKQT
                     IEEGHLTKQLQWLEGFCNDEDYAMVDIAAALVKLSLGEEMKEEIIEEKPRRERGDRKG
                     GTGAKDGMIRLFINIGRNQRVQAKDIVGAIAGEVGIPGKVVGTIDIYDKYTFVEIPKK
                     DAKTVIEKMKDIKIKGNKINIEKANKKKK"
     gene            8995..10980
                     /locus_tag="KR250_002281"
     CDS             8995..10980
                     /locus_tag="KR250_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBH0068212.1"
                     /translation="MSMTYMDPRVFLILEMVLEEDTNTLEQFSNVLGVSTRTIRNYIN
                     QINRELKGGIAKIVKNEKGTYSIRIEDESKLIEIVNFNRGKLSNFINLNSPDERINYV
                     LDILTMTNNPITIDDLAEEICIGRTTLIKDLKKADRILAEYDLELKRKPNIGMILVGE
                     EMDIRLLILDRLYENYIDVLENVNQINLVKDYDIECLRDELKKLFKKEELYITEQVLR
                     DVERYIIMSVLRNLNGYKFEKIDKRFEVIRCSDEYTLGLKLKALLEKIFNIVLNEKET
                     IFLTMPLIGRKAPSTKLALSSIKINDSVKEVVDEVTSFLLETSGIDFKEDKKLIENLE
                     YHLYFALNRMRFNIRVKNPLLQEIKERYTFPYSVAKIAAMIIEEKFDLKICDHEIGYI
                     ALHFGSYFEKNSRKVLNVNRVAVVCGTGLGTAQLIRIKIGKILGESVEVNTFSDIEAK
                     ANLDLLKYYDLVFTTVDFKTDLDVPIFRVNALFNEESIKKEIENAILLKNSINLKSIK
                     KIEKNIPFIGKLIEEDKFFILNKSTFMENLEDMLEKLVHKGIIDIEFKKKVLKREKKS
                     STALDNYIALPHSVNTNGENLYLAFGILEKPVIWDTKEIRIIILMIIPDKNVDSTDLI
                     IKSYEEVLEVGRDKKMVEELAKVKSFDEFYKIITKRR"
     gene            10985..11419
                     /locus_tag="KR250_002282"
     CDS             10985..11419
                     /locus_tag="KR250_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HBH0068213.1"
                     /translation="MDPVIFIIASLITVYLLNIFLGYLQLKDFNKNYIELKRKGRVAI
                     GRKKGRISSGTIVLILINDDGVIVETRKMQGVTVLARVKSFEGLVGKSLEYIEKSDLT
                     VYNKLLKKAILDAVKQYITFEKNKCEDTNNLSTDEECRMEVV"
     gene            11448..12008
                     /gene="srlA"
                     /locus_tag="KR250_002283"
     CDS             11448..12008
                     /gene="srlA"
                     /locus_tag="KR250_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418408.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HBH0068214.1"
                     /translation="MTQFFQVLASGADSFMNLFRAGGQQFVGFVTDIVPLLVALLVTM
                     NAIINFIGTDRVERLAKKCSANIFTRYLILPVLGTFVFANPMTLSLGRFLPEKFKPSY
                     FAAASFSCHTMNGLFPHINPGELFIYLGIANGITTLGFSTADLAVRYLLVGIVANFIK
                     GVVTDYTTKFVEKQQGIALNSDIKIG"
     gene            12072..12440
                     /locus_tag="KR250_002284"
     CDS             12072..12440
                     /locus_tag="KR250_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIB"
                     /protein_id="HBH0068215.1"
                     /translation="MAFNKVKIVKGSGGWGGPLIIEPTEKKNKVVYVTGGAKPETAVK
                     IAELTGCELIDGFSQGVKDDEIACVIINCGGTLRCGIYPQKKIPTINIMKTGKSGPLA
                     MFIKEDIYVSGVKPTNVEQL"
     gene            12465..13031
                     /locus_tag="KR250_002285"
     CDS             12465..13031
                     /locus_tag="KR250_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003374365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HBH0068216.1"
                     /translation="MAYDTKKKISEQSQGIVAKIGIALGNVTSTLFQAGRDTIDTLIH
                     TILPFMAFVAVLIGIINQSGIGNWVANLLTPLIGNVGGLVVLSIICSFPLLSPFLGPG
                     AVIAQIVGVLVGTEIGKGNIPPSLALPALFAINSQAACDFIPVGLGLAEAEPDTVQVG
                     VPSVLYSRFLTGAPTVLLAWLASFGLYK"
     gene            13072..13437
                     /locus_tag="KR250_002286"
     CDS             13072..13437
                     /locus_tag="KR250_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HBH0068217.1"
                     /translation="MELIYSTQINKIGDNANEFLQHNMFVTFKDNAPQELENYCYIHS
                     ENNLVKEILTDDVLSINNVDYKITSVGELVNQNLSELGHITFKFTGEKDGAIGGTLYL
                     EEKEIAPIGVGTILKIIRK"
     gene            13538..14311
                     /gene="srlD"
                     /locus_tag="KR250_002287"
     CDS             13538..14311
                     /gene="srlD"
                     /locus_tag="KR250_002287"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HBH0068218.1"
                     /translation="MRKVALVVGGGRSLGEYLSKHLAKVGYDVAIADLNEDNAKKVAE
                     EIKTEFGCKSVGYSCNAIDERQVISTVKAIENDFDRIDLLVYNAGVAVSKKITDFGYK
                     DFKFIVDVNLNGYFLFAREVSKVMIAKGIEGRIIQINSKSGRVGSKHNSGYSAGKFAG
                     VGLTQSLALDLAEHKITVNALMLGNLLDSDMFESLIPQYAVKLGIPESEVKQVYIDKV
                     PLKRGCRFIDVANMLTFYASKEAEYCTGQSINITGGQVM"
     gene            14660..17098
                     /locus_tag="KR250_002288"
     CDS             14660..17098
                     /locus_tag="KR250_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBH0068219.1"
                     /translation="MDIIKKLSHGCTLDCHDCCKFNVYTKGNNVVKIEGDKNHPYTKG
                     FICKKGMAHLDRLNHKDRIKTPMLKVDGVWKEISFDKAIEIMAEKLTYYKEKYTSKSV
                     MHYDQYGSGSVLKYIGDIFFNFYGGVSRHKGGPCWSAGMHAQKYDFGVAKSHAIEDML
                     NSKSIFVWGKNPAYTTIHTMQIIKKAKEKGIKIVVIDPIYTKTAQIADKYVQVNPGTD
                     GALAIAMAKIIVEDKLYDEEYINSYVIGFEEYKKYLSSLELSFLIDECGVKENDIREL
                     VDLYTNKYSSINVGYGLQKYKNGGNTIRAIDALGAITGQIGFSGGGVNYANKVYPSVI
                     NSDPYNSQSYGEDREFYVSNISKFIEESLKNTSNKVNYASDELDMALHKVNYISDELD
                     TTSNKVNYVSDELDMALHKVNYVSDELDTTSNKVNYVSDELDMALHKVNGVSDELDMA
                     LHKVNCVSDELDMTSNKVNYVSDELDMTSNKVNYVLDELDITSNKTDYISNELYNLSN
                     KSIKDNIPIKMAVITKSNMLNQLPDLVELERVFSKIEFKVCFDMFITDTVTLCDMFIP
                     CTNTLESEDIIYSSMTNPYITYNERAVKPAHKLMDEYYFFMELAKKMGLNDYPFVEKR
                     TYLEKVIEPLKRFDKNLDIEKLKNNYFTIHNPVAWEDKKFETPSGKYELYSESIKNLG
                     ISPTPVYISNKYKEIEDKNISFRLLTNHHADTLFSQHFMDKKSIAQAYINQRMAKKVG
                     IEEKDIVILRSKKAKINVQINIDDGVGNYIVKMYVGWWKKHGNPNSLTDTGISDFGGQ
                     VTYNESMVEIIRQN"
     gene            17186..17521
                     /gene="spoIIAA"
                     /locus_tag="KR250_002289"
     CDS             17186..17521
                     /gene="spoIIAA"
                     /locus_tag="KR250_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor antagonist"
                     /protein_id="HBH0068220.1"
                     /translation="MVNYSLEHKNLYIEFMCSELDHHVANEIREEIDNLLSVNQVKNV
                     VFNFENINFMDSSGIGVIIGRYKKISNEGGRVSVINISSRVKKIFDLSGLNKIIGIYD
                     TYEEALSSL"
     gene            17535..17963
                     /locus_tag="KR250_002290"
     CDS             17535..17963
                     /locus_tag="KR250_002290"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor"
                     /protein_id="HBH0068221.1"
                     /translation="MNNIMEVKFSAISENESLARVIVASFAAKLDPTVDELVDIKTAV
                     SEAVTNAIIHGYEEDSSKFVFLRCEIEGNTIKIIVEDEGYGIENVEKAMEPLYTSKPE
                     LDRSGMGFTVMKSFMDDVEVSSVKDNGTRIEMTKKINVPK"
     gene            17986..18750
                     /gene="sigF"
                     /locus_tag="KR250_002291"
     CDS             17986..18750
                     /gene="sigF"
                     /locus_tag="KR250_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigF"
                     /protein_id="HBH0068222.1"
                     /translation="MEVTVAREEKKPLLSHEETLELIEKVQNGDEEAKEILISSNLGL
                     VRSVVSRFLNIGYDREDLFQLGSIGLIKSIYKFDPKFNVKFSTYAVPMILGEIKRYLR
                     DDGMIKVSRSLKQIAVKAKMESEALTKKLGREPSIEELAKAIDVEKEDLVMAMEANFN
                     VEYLQGVIHEEEGSPICLIDKISMKGESEEEKVVDNILLKDILGRLDKRERQIIVLRY
                     FEDMTQSEIGEMLNISQVQVSRIEKKVLSKLKEYIS"
     gene            19030..19467
                     /gene="spoVAC"
                     /locus_tag="KR250_002292"
     CDS             19030..19467
                     /gene="spoVAC"
                     /locus_tag="KR250_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AC"
                     /protein_id="HBH0068223.1"
                     /translation="MDKNYKKYVDQISPKPTYLKNYTLAFIVGGIICMIGQAINDLYM
                     KVGGLDKLGASSATSITLIFIGAFLTGLGVYDLIGKRAGAGSIIPITGFANSIVSPAM
                     EYKREGYVLGVGANLFKIAGPVLVYGIGSSILCGIIYYIFKMF"
     gene            19634..20647
                     /gene="spoVAD"
                     /locus_tag="KR250_002293"
     CDS             19634..20647
                     /gene="spoVAD"
                     /locus_tag="KR250_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AD"
                     /protein_id="HBH0068224.1"
                     /translation="MKNKRIGKRTVKLENKPTIISTGTIVGPKEGEGPLKDYFDMIMT
                     DDLYGEKTWELAESKMVETASQQAIQKAGKKLSDVNYMLGGDLINQIVPASFAARELA
                     IPFLGIYGACSTMAEGLCIGSMLIDGGFADLVLSGTSSHYCTAERQFRFPLELGNQKP
                     MTAQWTVTGAGSVLLAPNGDGPKVKYVTVGKVIDEGIDDGNNMGPAMAPAAIDTIYSY
                     FEDTNDDPNSFDIIATGDLGTLGKQIAEDFLKEKGVDISKVYTDCGIEMFNLKEQDVH
                     CGGSGCGCSATVFAGYIYDKLRKKEFNKVMLVSTGALLSPTSTLQKQTIPCVAHAVVI
                     VNE"
     gene            20661..21023
                     /gene="spoVAE"
                     /locus_tag="KR250_002294"
     CDS             20661..21023
                     /gene="spoVAE"
                     /locus_tag="KR250_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AE"
                     /protein_id="HBH0068225.1"
                     /translation="MLMDYVRVFIVGGIICLIAQVMMDYFKMQTPYVMVTYVTTGVVL
                     TFLGLYEPLVKFAAAGATVPIIGFGYSLATGVIKSIQSDGFLGIFLGGTTATAGGIAA
                     AIFFGYIMSVVFTPKAKP"
     gene            21206..22222
                     /locus_tag="KR250_002295"
     CDS             21206..22222
                     /locus_tag="KR250_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBH0068226.1"
                     /translation="MVTIKDIARNLGISYSTVSRCLNNNPNVSEKTKKKVVEEANRLG
                     FHFNVNARNLAKKETNRIGVIFSNNFNHQDTRKFFSDIMDSSINSIETNKYDFIIQPN
                     NNISGDSNVYKMVNGQMVDGLVIVSQSIKKEEYDFLKDNNFPHVFIYFKPSFIGEVDN
                     FFWDDNVYGGYIATKHLIDHGHKDIITVTSDDKSSKMHEDRTKGYLNAMEEANLKTEV
                     IKSKMDFESQVEFLEKYIDKIKKASAIFVQQDVPAISIIQELKTTYGINVPEDISIIG
                     YNNIELISYFRSHLSTIDDPREQVIKNGVDSLVNIINKKSTEHIPRKLYPRLIIRNSV
                     KRIK"
     gene            22597..23439
                     /locus_tag="KR250_002296"
     CDS             22597..23439
                     /locus_tag="KR250_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3100 domain-containing protein"
                     /protein_id="HBH0068227.1"
                     /translation="MKEKAGIRNAIIAAFIITILAELIGPISFKVMGINVTLLSILWA
                     IFIGMAVSPHLLGRVIPALKKFIGDNEINVSPYLLSLTLYPLGIMFGINAGPKVGILL
                     QAGPALLLQEFGHMGTMLIALPVGVALGLGRSALGGTFSLCRDTALGIIGDKYGLNSP
                     EGIGTLGTYISGSIFGTLLFSFLAPVGVLLGFHPYALAMASGMGSGSMMGAATASLMN
                     TVPQMSEQILAYSATSGLITAVTGIYIELFLSLPVANYYYSKVAPVIERFRGRKTRRT
                     SNKL"
     gene            23454..23918
                     /locus_tag="KR250_002297"
     CDS             23454..23918
                     /locus_tag="KR250_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0068228.1"
                     /translation="MEKTFKTIMEQIKIVLFIYIIICFGQMIALKTQAQDLLIGSVSG
                     FLLVIIAIIIKNFVKKPNLPGFAWATLVAFVLSLPISPVGDIIATNVGKINFMSTVTP
                     LLAFAGISVGNQLDILKTLSWKLVVISFIVMTSTYFGSAFISQTVLRLNGML"
     gene            23905..25077
                     /locus_tag="KR250_002298"
     CDS             23905..25077
                     /locus_tag="KR250_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBH0068229.1"
                     /translation="MECFKEVVNMNELKAKIKEEIKLLSEEKKTSFEKVSDYLFSNPE
                     LAFEEYKSQKALCDLLEENEFNVTKGVGGLETSFEAVYSNGTNGKTVAFLAEYDALPG
                     MGHACGHNIIGTSSVGAGIILKEIMKKHNIEGTVKVFGTPAEERVGGKITMIKEGVFN
                     NVDAALILHPSDASMPDDISFAQVNLKFDFTGKASHAAAFPWEGKSALSGVIALFNSV
                     NSMRLHLKDYARVHGIITDGGSIHNIIPEKSTAIFNVRALSIEYLNEICEMLKNCAKG
                     AAISTGTNVEIVQLDEIYKEIKNDSELVNIVRQNFEVLGEDYVERDLSQGIGSTDTGN
                     LTHEIPAIQAYIKLKENTATHTDEFAVAAGGEEGRVALIKAIKVLAMCGVDILYSK"
     gene            25121..26056
                     /locus_tag="KR250_002299"
     CDS             25121..26056
                     /locus_tag="KR250_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1177 domain-containing protein"
                     /protein_id="HBH0068230.1"
                     /translation="MILKQVIEVYDILDKANANGEEVKTYLQGYGEVDVTVKELSSSK
                     GSTDLVKLTIPGKNGKLKGGDAPTLGILGRLGGIGARPEVIGFVSDGDGALVAIAVAA
                     KLLDMQRKGDILDGDVVISTHICPDAPTKEHYPTPFMDSPIDMMTMNENEVDSSCDAI
                     LSIDTTKGNRVINTRGFAISPTVKEGYILKTSDNLLDIMQTVTGKSPFVFPLSIQDIT
                     PYGNNLYHLNSILQPAVATDAPIVGVAITTEMPVAGCATGATNFSDLDSAGRFAIEVA
                     KLFGRNKCDFYDKEEWEMLIKRYGKLNRFQTFGIQ"
     gene            complement(26330..29023)
                     /locus_tag="KR250_002300"
     CDS             complement(26330..29023)
                     /locus_tag="KR250_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HBH0068231.1"
                     /translation="MNNDNDKNGNKIRRKKVSSSGNTNKPINRTSANKTRSTKNKKKS
                     KKSDKFKKLRVFGIVFLVLLVVGTAGTAGLVFASLRDVTPVTEAVLDKQTNQTTTIKY
                     ANGKTLSTAPSVNKKTPVPLDKISPYLQHAVIAIEDERFYEHKGVDIKGLFRSVLKTL
                     TGTKQGGSTIPMQVSKMLLTTEQQTIPRKIKDIYYAHEMSKTVSKDKILETYLNNFFV
                     GRGLAGAEAGARGYFDKSAADLTLAESALLAGSTKNPSRFSAYKTSKLEGNETKEDLE
                     NKLLFFVNTTDDDLDDPTQVDFDMIEKIKSWELISNDTYRQLKAGTLVVRKAVSNPEA
                     KKRQEIVLKKMLELKYIKQSEYDEAIKAKIEIKLPQSSDKVSSSVEDLIESEVINALM
                     EQGHTNDEAQNLFYNGGLIVNTTIDPKMQDALEEEFDRNSNFPGHMVGPDGVSQPQAA
                     MVILDYKNGEIRALAGGRNISGRKTLNRATNPHQPGSSIKPLAIYTPAIDTLKITQAT
                     ALSDSRGGYKFKENNEWNPRTTTAGHGSMSLRKALAKSSNTIAVKTAEMLGDSYDECV
                     DIMMDYLKNFGITTLKNNHSGSSEASDRKFPSLTLGGMANGITPLQMAAAYGTLANHG
                     IYVEPSIFTTITTFDGQLLVKNAPEEHKVVDPEVAYVVTDMLESVITEGTGGVATLPK
                     GMPVAGKTGTTNSAYDAWFVGYTPYYVGATYIGDDAGRKDDSGNTIKRREVPHGSTST
                     AKLWEKIMEKIHANLTVTEFEVPKNVYFTKINLEDGGKQSSGSKAAFIEGTAPTKVSS
                     QPSSEDTKKPDNNQPEENNNNNNNTGDNGGGSTPPDNGGNNGGGSTTPPDNGGNNGGG
                     STTPPDNGGNNGGGSTTPPDNGGNNGGGSTTPPDGGGTPAT"
     gene            29237..29935
                     /gene="yunB"
                     /locus_tag="KR250_002301"
     CDS             29237..29935
                     /gene="yunB"
                     /locus_tag="KR250_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YunB"
                     /protein_id="HBH0068232.1"
                     /translation="MNRIVDRKTKNEFRKIIAIFLVILFLSVFIGSFIYIDKTLRPTI
                     TVLAETKALELANRSINKAVAEMVEGKINYEDLMDIQLDNNGKITMIQANTIMMNEIA
                     SAIALEIQDELKKDKTASSYIPIGTALGSPILAKYGPKLEVSIEPIGTVSVNFKTEFE
                     SSGINQTRHRIYLEAQTQVKVVIPLITSTKQIKAQIPICETIIVGDVPESYVNIPEKN
                     LGNVLPNTGKNTNK"
     gene            complement(30151..31164)
                     /locus_tag="KR250_002302"
     CDS             complement(30151..31164)
                     /locus_tag="KR250_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S55"
                     /protein_id="HBH0068233.1"
                     /translation="MQLKNFHFKNNTLSKKTNLAKSKFTIVLTFLFLFLLYFFSNNLI
                     YAQNLQKNEPEYLIPIGNVLQIDAELKNIIVRNPGEASPFRLGDAIVKVNNAEVDGYS
                     DFANTLNALPEEKQVSILLNRGGQLITMKTTKHILEKISFNNLLSGFATLTYINPDTS
                     EFGAVGHPISVGNARKIPIKTGSISTTKHLNIEKSYKGSVGCINAKRENIIGSFTENT
                     DFGIRGQINNFDISKLKKYKVASLDEVKLGKAQIILQDNFNKCKKYNIEIIDIENQKH
                     PESKSFKIKITDKKLLAQTGGIVQGMSGTPIVQDNKIIGAVSHAIENDPAVGYGVFIK
                     WML"
     gene            31392..32276
                     /locus_tag="KR250_002303"
     CDS             31392..32276
                     /locus_tag="KR250_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBH0068234.1"
                     /translation="MKKYRLLVTCIIAMGIIIIGTSNPVSNFYKTTNLISSGNITNEK
                     ANKDENSNKNNKDKNEDKSNSKSNKDKSSEDKSSDNKNEIQKNKKLLICIDPGHQGKG
                     DSNLEPVAPGSSSKKARVSSGTEGIATKKPEYVLNLEASLVLKSILESKGYNVIMTRE
                     THDVNISNSERAILANDKKADMVVRIHADSLNNSSKTGASILIPEKDGKYTAPIYEES
                     NKCAEFIKQNMEQSGIQINGIVQRGDLTGFNWSKVPAVLVEMGFMSNYNEDQMMSNPD
                     YQRKMMQCIADGLDAYFK"
     gene            32478..34028
                     /locus_tag="KR250_002304"
     CDS             32478..34028
                     /locus_tag="KR250_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBH0068235.1"
                     /translation="MLVVENVSHGFGARTILENVSFRLRKGEHIALVGANGEGKSSFL
                     NIITKKLMPDAGNIKWSSRATVGYLDQHTVLSKGKTIREVLREAFKHMFDLEQEMIAM
                     YDKMGEASDDEMSKLLEETAEIQTILENSGFYMIDAKIQEVANGLGLGEIGLDKDVTD
                     LSGGQRTKVLLTKLLLENPTILILDEPTNYLDEEHITWLTKYLQEYENSFVLVSHDIE
                     FINNTCNVIYHMENGELNRYKGNYDEFVRLNDIKKRQEEQAYDKQVEERKRLEDFVAR
                     NKARVATRGMANSRQKQLDKMEILERPKEKIKPTFAFKDARAASKIIFETENLVLGYD
                     EALTKPLNFHLERGKKIALKGMNGIGKSTLLKTLLGIIKPFEGNVKLGDYLEVGYFEQ
                     ESSRENSNTPMDEVWSEFPGLTNFEVRQALAKCGLTNEHITSQMRVLSGGEAAKVRLC
                     KVMLKNINFLVLDEPTNHLDVEAKDELKKAIKEFKGTVLLVCHEPEFYSEIVDDVWNI
                     EDFTTKIV"
     gene            34057..>34552
                     /locus_tag="KR250_002305"
     CDS             34057..>34552
                     /locus_tag="KR250_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HBH0068236.1"
                     /translation="MKKFLNVLFSICISIVIIVGVINFTVGFKQLYYFDIDYLNISEL
                     SGLSKDDIKLNYDYLIDYNLNKNVSEFKLPTLKSSPQGKIHFEEVRNIFQNINKLAKL
                     LLVVSLVGIILSVKNKNIKILKTTSITLIIMPLLLTVPILLNFEKSFIIFHKLLFRND
                     YWIFN"
BASE COUNT        13155 a         4092 c         5923 g        11382 t
ORIGIN      
        1 tcttacaaaa tcaatgaaaa taagtatatc cattttaaag tacataaatt taaaaatcca
       61 gaaaaatata cttaaattat gtaagtaaaa gaggggttga atcagttgaa aatgtaatat
      121 tagcaaatag tacatcaatc ctatataccc tgtaagcaac tctattttca tgtactaaaa
      181 atgtacctat tttattaact gaaaagaata aattaaatgt aagtacataa gaagatagaa
      241 aaaattaaaa acaaaaaata tttatataaa taaatataaa aaatgatata atccctatta
      301 aaaaatttat agatgatgaa gaaatatcaa aatcttatat tataggaaga gacacttctt
      361 tgccaaaatc tatagaaaaa caataccaaa tccaaagaga ataggcggag aaaaaaataa
      421 aataaattca agagtaatag aaaagtttta tagaataaag aactgataac tctttatata
      481 acgaaaaata gaattttaag agaaaatgat ttaataactg atttaacatg gtgttttagc
      541 aggtaaaaat aaatcaccag taatgattat agaaaaatta ctttatgaat ctcaaaaaac
      601 ttagttaaga gtaagagatt tgataagata actaaagttg gggaaatgtg tttgatgaga
      661 caaaaaatta gtaaaatagt ataatctaaa taaggatatt ttgttgatta aactacacta
      721 atagtaatat tgtaggggga aaagatgaga gaagaatcga actctttaag tttagaccat
      781 atagaaattt tctttgaact tttatctcaa agtacagaag attatatatt tttttgggat
      841 ataaatagga ataaatttaa aatttcttca gcaatttttg atgaatttaa cttaagtaaa
      901 gagatagaat ctgatttggt caattgttgg agtaaaattg tttatccaga tgatgttcaa
      961 atatggaaag atgatataca agaattacta catggaaaaa aaggtgagca caatttagaa
     1021 tataggttga ttaataaata taaagaaata gtatggattt cttgtagagg taaagtttat
     1081 gtgtcggatg acccaaaaac tatatttcta gtcgggagaa taaagaatat tggtgaaaaa
     1141 aataaatttg attctatcac aggaacgtgg aatagagaac aatttgaaca taaaatgaat
     1201 tatcttataa aagagaaaat ttataaaaat ggtgctatgt ttattatgga tattgataat
     1261 tttaaaaata taaatgaaaa atatggacat tcttatggtg acaaagtgtt gcgtgcaatt
     1321 gctactgaag tactggaata tcttccaaaa gatgtaagat tgtataggtt agatggagat
     1381 gaatttgcat ttttttaccc tatgtgtact aaagaaacta tagagaaaat atatgaaaag
     1441 atacagatgt atacaaatac tcagcatgaa attgaatcaa gtaagtacta ttgcacagtg
     1501 actgcaggcg ttgctatgta tccagaagat ggagataatt atttagattt atttaaacat
     1561 gctgatattg cattggatat agcaaaaatt agtggtaaaa atagaattaa gtttttttca
     1621 caagagctct atgaaaataa actaaaggta atttcaatgc aacaaaaact aagagagtgc
     1681 gttgaaaata attttaatga ctttgagctg ttttttcaac cccaggtaaa tgctgttaca
     1741 aaagaggtaa taggggcaga ggtactttta agatggcatt cgagtactta tggagaagta
     1801 tctcctgttg aatttatacc aatactagaa cagagtaatt taattatacc agttgggaag
     1861 tggattataa aagaagctgt aaaacagtgt aaagaatggc ataaaataaa tccagacttt
     1921 aaaatatctg taaatgtatc gtatattcag ttaaaggaag atttttttag agattttata
     1981 gtagaatgtt tagtagagta tcaattaaga cctgaatttt taattttgga attaacagag
     2041 aattgttgga taccagatat aaatttacta aatgataaat ttattagttt aaagggtata
     2101 ggagtatata ttgcaataga tgactttggt acaggatatt catctttaaa ttaccttaaa
     2161 gaactgtctg ttaatataat aaaaattgaa agaagttttg ttaagaatat tacatataat
     2221 agttatgaat atacttttct tgaatatatt attaaattag ctcatattat aaatttaaaa
     2281 gtttgtgttg agggaataga gtcttatgaa gagtatgata tagtaaaaag cttaggggta
     2341 gatattattc agggtttttt atttggaaga cctgtaagtg cctctgaatt ttataaattt
     2401 cagctatcaa agtgatgtat ataaaatgag atatatagta tataaaaata gggcaaagtg
     2461 gaagttttga agtttattaa aaatttacac tttaccctat tttgttatgt cttctataaa
     2521 ggattaatat actacactaa ctattatata ctttcactcc aaatttagag attatttcac
     2581 tagctttttg gacatcttcc ttagtggcat caggaacatc ttttagttca taatcgatac
     2641 ctaaactttc ccatttgtgt attcccatag aatggtatgg tagcaattca aatctttcta
     2701 catttttcaa tgtagctaca aattgaccta gtttttctaa attatctata tcatcagtaa
     2761 tgccaggaac taaaacatgt cttatccata caggaatatt tcttgattct aagtgtctag
     2821 caagttttaa tgccttatcc ataccaacac cagtcaaatc aatagatttt tcctcaacca
     2881 tatgttttaa atcaagtaaa accaaatcag tattatctaa aactgggtct atagtttcta
     2941 tatctacaaa tccagatgta tccaagcaag tgtgtatatc attttctttt gcttttttaa
     3001 ataattcact taaaaattct ggttgaagag tggattcacc accagaggca gttatacctc
     3061 caccagaaaa cttcataaaa gaagtatact taagagcatc cgttattatt tcatcaactg
     3121 tgtattcttt tcctgattta atatcccatg tatccctgtt atgacagtat ttgcatctaa
     3181 gaggacaccc ctgaaaaaat aatatatatc ttattcctgg tccatctaca gtaccaaacg
     3241 tttctataga atgtatcttt cccttaatca tagagttcac ctcacaatat attttaataa
     3301 ccttatcaat agttataata tttctctaaa aaaggccatc ttataccaga aataattcaa
     3361 atataagata gccttttatt tttatttttt acacagtagt aatcttagtt aaagtataaa
     3421 aatttaaaga ctaagccatt ttaccgtgga atgttctgtt tataacatct aattgttgct
     3481 ctttagttaa ttttatgaag ttaactgcat atccagatac tcttatagtt aattgtggat
     3541 attcttctgg atgttccata gcatcttcta aagtagctct atcaaataca tttacattta
     3601 agtgatgagc attttgagcg aagtatccat ccatcattgc agaaagattg tttattcttt
     3661 cagtcatatc ttttcctaaa gcagcaggta ctatagagaa agtattagat ataccatctt
     3721 gagcatgttc atatggtaat ttagcaactg aagataatga agctaaagca ccattagtat
     3781 ctctaccatg cattgggtta gcaccaggag caaatggagc accagctctt ctaccatctg
     3841 gtgtattacc agttttctta ccataaacaa catttgaagt tattgtaagt atagattgag
     3901 taggataaga atttctataa gttttattct ttcttatttt attcataaag ctttcaacta
     3961 actctacagc tatactatca actctatcat cattatttcc gtattttgga taatctcctt
     4021 ctatttcaaa atcaacagct attccttgtt catttctaat agttttaacc ttagcatgtt
     4081 ttatagcaga taatgagtct gcacaaactg ataatccagc cataccacaa gccatagttc
     4141 taaatatatc tctatcatgt aatgccattt ctaatgcttc ataagaatat ttatcatgca
     4201 tataatgtat tacatttaaa gtatttacat ataatgttgc taaccaatca gtgaatggtt
     4261 caaatttact cataacttca tcataatcta agtactcaga agttataggt tcaaatcttg
     4321 gtccaacttg aacaccagat ttctcatcta caccaccatt tatagcgtat aataaagttt
     4381 tagctaagtt aactctagct ccaaagaact gcatttgttt accaattctc attgcagata
     4441 cacaacaagc tataccatag tcatctcccc agtatgctct cattaaatca tcattttcat
     4501 actgtactga acttgtatct atagatactt tagaacagaa atctttgaat ccttgaggta
     4561 atttagttga ccatagaact gttaagtttg gttctggaga aggccctaat gtatatagag
     4621 tatttaacat tctaaatgag tttttagtaa ctaatgttct accatctatt cccatacctc
     4681 ctatacactc agttacccaa gttgggtctc cagagaataa gttattgtaa tcaggagttc
     4741 ttaggaactt aaccattctt aatttcataa caaaatggtc catatactct tgtaattctt
     4801 cttctgttac gattccagct tttaaatctc tttcaaagta tatatctaag aaagtagatg
     4861 ttctacctaa agacattgca gcaccatttt ggtctttaac agcacctaaa taaccaaagt
     4921 ataaccattg tacagcttct tttgagttag ttgctggttt tgatatatca aatccataag
     4981 attcagccat ttttttaagt tcatttaaag ctgatatttg ctcagttatt tcttctctta
     5041 atctgataac ttcctcatcc atgcaagaaa cttctaatga tttcttttgc tcatttttat
     5101 cttctattaa agcatcaaca ccatatagag caactcttct gtagtcacca attattctac
     5161 ctctaccata agcatctgga agacctgtta ttataccaga ttttctagca gctctcattt
     5221 ctggagtata aacatcaaaa actccttggt tgtgtgtttt tctgtattta gtaaatatat
     5281 cttttatttc tgggtctaat tcatatccaa aagcttcgca tgaattttca accatcttta
     5341 taccaccata tggcatgaca gctcttttta aaggggcatc cgtttgtaac ccaactattg
     5401 tttctaattc tttatctaaa tatcctggag catatgcagc tattccagaa acagtttttg
     5461 tatcaacatc taaagtacca ccattttctc tttctttttt aaataggacc attgcttcgt
     5521 cccataattt tttagtattt tcagtagcac cagctaagaa agagtcattt ccctcatatg
     5581 gagaatagtt taattgtata aactctctaa catttatttc tttagtccat cttcctgttt
     5641 taaatccttg ccatgcattc attaagtatt tcctccttag atttaaatga taaataatac
     5701 tattaaaatt aatttttata tacttaattt attttatata tttttttaaa aatatatttt
     5761 ctaatataat aatatcatat tattcgtatc ctgtatacat aaaaaataaa aaattgtcgc
     5821 tttaaagtat aaaacagatt atatgttaat aatgtggtga aattataccc atatataaat
     5881 aaaataaaac aaataaatag ctaattaagg agaaaatatt ttttattttt tgtttaggat
     5941 aaataaattt tattatggaa aaaagaaaaa gcatacatct tcacaaattg aatatttatt
     6001 taataggggg aggtgataga ttgtttatca cagtggtatt atttttagta ggtttcctat
     6061 taattactaa aggcgcagat atctttataa attgcactgt agagataggt aaaaaaacga
     6121 atatatcaga aattatactt ggagctacta tagttagttt tgcaacaaca ttacctgaat
     6181 ttacagtgtc tctacttgct tctattgatg gtcatactac tatgagttta ggaaatgcag
     6241 ttggttctat aatatgtaat acaggattag cattagggtt ggttgttttt ataagtccat
     6301 ttaatgtaga taaaaaaatg tttttttcta agtctttact attaatagta tccgttatag
     6361 ttttaatttt attgagttta gatggagtta taacaagagg tgattcatta ttacttataa
     6421 taatattaat cttttatatg gttaataatt atagaagtgt cgttggaaaa tctgatacta
     6481 aaaatagaaa tattaaaaat aacactatta aagacatatc tactaaaaaa gggaaaaatt
     6541 atagaggttt ttccgtatta gaaattgtaa aaatactatt actttttgct acaggattga
     6601 taatgatgat aataggttca caaatcctta tagagagtgg agtaataata gctagttttt
     6661 taaatatacc tcaaggtata gtaagtttaa ctataatagc acttggaact tctctgccag
     6721 agatagtttc gtcgattact gctataagaa aaaatcatca tgaaatatca gtaggaaata
     6781 ttttgggtgc aaacatattg aatatagtat cagtaatagc tgtatcagca ataccaaata
     6841 acataccaat actatctcaa aatagacaat tggatatacc attcatgata ttattattat
     6901 taatagtaat aataccaaca cttaaatcta atagacttag tagaatacaa ggtatattga
     6961 tgttattcac atattttctg tacatatcaa ttttgtattt catgtatatt atttaactat
     7021 actgtttaaa atattaattg cagaatatcc taaaaaagta tataatggat aggttaagat
     7081 taattgataa cggagaggaa taatatatga atataacaaa gtttgaagat ttaccaataa
     7141 gtgagggaat aaaaaaagct atagctgaaa tggggtttga agaaccatct cctatacaag
     7201 cacagtctat acctgcgatt ttgtcaggta aagacgttat aggacaagct caaactggta
     7261 cgggaaaaac agctgctttt agtatcccta tactagaaac aatagaccca aataatagaa
     7321 gtttacaagc tgtagtgctt tgtccaacaa gagaactggc aatacaagta tcaacagaaa
     7381 taagaaagtt agctaagtat tcgcacggaa taaagacatt acctatatat ggtggtcagc
     7441 caatagatag acaaataaaa tctctaaaga gtggtgtgca agttgtaata ggtacaccag
     7501 gacgtacaat agaccatatc aatcgtaaaa cattaaagat ggataatgta aaaatgatta
     7561 tattagatga agcagatgaa atgctagata tgggatttag agaagatata gaaatgattt
     7621 taagtaagat tccagaagaa agacaaacaa ctttcttttc agctactatg ccaagaggaa
     7681 ttttggaatt aactaagaga tatcaaaaag acccagagca tataaaagtt gttagaaaag
     7741 aacttacagt ttcaaataca aagcaatact atatagaaac taggtcatca aataaattag
     7801 aagtattatg tagattagta gatgtatatg accctaaact atctgttgtt ttctgtaata
     7861 ctaagagaaa agcagacgaa ttggtaggag atttacaagc aagaggatat tttgcagatg
     7921 cattacatgg tgacttaaaa cagactcaaa gagatatagt aatggataag tttagaaatg
     7981 gtacaataga tatacttgtt gctactgatg ttgcagctag aggtatagat gttgatgatg
     8041 ttgaatgtgt attcaactat gacttaccac aagatgaaga gtattacgtt catagaattg
     8101 gtagaactgg tcgtgctggt agagaaggta tgtcatttac ttttgtattt ggaaaagaaa
     8161 tgagaaagat gaaagatata gaaagatata ctaaatctaa actaataaag cataatatac
     8221 caacaataac tgatgttgaa gagaaaaaag taggtacttt ctttgcacaa gttaaacaaa
     8281 ctatagaaga aggtcactta actaaacaat tacaatggtt agaaggtttc tgtaatgatg
     8341 aagattatgc aatggttgat atagctgctg cacttgtaaa actatctcta ggtgaagaaa
     8401 tgaaagaaga aataatagaa gaaaaaccta gaagagagcg tggagataga aaaggtggta
     8461 ctggagctaa agatggtatg attagattat ttatcaatat tggtagaaat caaagagtac
     8521 aagcaaaaga tatagttggt gccatagctg gagaagttgg aataccaggt aaagtagtag
     8581 gtactataga tatatatgat aagtatactt ttgttgaaat acctaaaaaa gatgcaaaaa
     8641 cagtaattga aaagatgaaa gatattaaga taaaaggtaa taagattaat atagaaaaag
     8701 caaacaagaa gaaaaaataa cgtatagtat acaaaactat aattagaaaa tatataaaat
     8761 aatatattgg atatttaaaa agcctgatgt gttttcaaaa cagatagtag ataatctgta
     8821 agagttaaca gcataaggct tttcttgttt tatatgatgt tataattttc cagattgttg
     8881 tgaaaaatgt atattagttc ctttttttca aatctgattt gaaaaagaga atattaatta
     8941 tctatatagt ttagattata ataaaaaatg taagataaca aaggggagaa aaagatgtca
     9001 atgacttata tggaccctag ggtttttttg attttagaaa tggtgttaga agaagacaca
     9061 aatactctag aacaattttc aaatgttttg ggtgttagta ctaggactat cagaaactat
     9121 ataaaccaga ttaatcgaga attaaaaggt ggtattgcta aaatagttaa aaatgaaaaa
     9181 ggtacatata gtataagaat tgaggatgaa agtaaattaa ttgagattgt aaattttaat
     9241 agaggaaagc tatcaaattt tataaattta aattcacctg atgaaagaat aaattatgtt
     9301 cttgatattt taactatgac caacaatcca attaccatag atgatttggc tgaagagata
     9361 tgtataggaa gaacaacatt aataaaggat ttaaaaaaag cagacagaat tttagctgaa
     9421 tatgatttag aattaaaaag gaagccaaat ataggtatga ttttagttgg tgaagaaatg
     9481 gatataagat tattaatcct tgatagattg tatgaaaatt atattgatgt cttggaaaac
     9541 gttaatcaga taaatttagt taaagattat gatattgaat gtcttagaga tgaattaaaa
     9601 aagttattta aaaaagaaga actctacata acagaacagg tcttgagaga tgttgaaaga
     9661 tatataatca tgtctgtctt aagaaatcta aatgggtaca aatttgaaaa gatagataag
     9721 agatttgaag tgataagatg ttcagatgaa tatactttag gattaaagct caaagcttta
     9781 ttggaaaaga tatttaatat agttttaaat gaaaaagaaa ccatattttt gacaatgcca
     9841 ttaataggta gaaaagctcc tagtacaaaa ttagcactta gcagtataaa gataaatgat
     9901 agcgtaaagg aagttgtaga tgaagtaact agttttttat tagagacctc agggattgat
     9961 tttaaagaag ataaaaagct tatagaaaat ttggaatatc acttgtattt tgcacttaat
    10021 agaatgagat tcaacataag ggtaaaaaat cctcttttac aagaaataaa ggaaagatat
    10081 acatttccat atagtgttgc aaagatagct gcaatgataa ttgaggaaaa gtttgacttg
    10141 aaaatatgtg accacgaaat aggatatata gcattacatt ttggaagtta ttttgaaaaa
    10201 aattcaagaa aagttctaaa tgtaaatagg gtagctgttg tttgtggaac aggtcttggg
    10261 actgcacaac ttattagaat aaagatagga aaaatacttg gagaaagtgt agaagtaaat
    10321 actttttctg atatagaagc taaagctaat ttagatttat taaagtatta tgatttagta
    10381 tttacaacag tagatttcaa aacagactta gatgttccaa tttttagagt aaatgcgtta
    10441 tttaatgaag aaagtataaa gaaagagata gaaaatgcaa tacttttgaa aaatagtata
    10501 aatcttaagt ctataaaaaa gattgaaaaa aatatccctt ttataggtaa attaattgaa
    10561 gaggataagt tctttatatt gaataagagt acgtttatgg aaaatttaga agatatgtta
    10621 gaaaaattag ttcataaagg aattattgat atagagttca aaaaaaaggt attaaaaaga
    10681 gaaaagaaat cttcaacagc attagataat tatatagcat tgcctcactc agttaatact
    10741 aatggggaaa atctttatct ggcatttgga atattagaaa aaccagtgat ttgggataca
    10801 aaggaaatta gaataataat attgatgata attccagata agaatgtaga tagcacagac
    10861 ctgataataa aaagttatga agaagtactt gaagtaggga gagataaaaa gatggtagaa
    10921 gaacttgcta aggtaaaatc atttgatgaa ttttataaaa taattaccaa gaggaggtaa
    10981 taatatggac ccagtaattt ttattatagc atcacttatt acagtgtatt tattaaatat
    11041 ttttcttgga tacttacagc ttaaagattt taataaaaat tatattgaac taaaaagaaa
    11101 aggaagagtg gcaattggca gaaaaaaagg aagaatttca tcaggtacaa ttgtgttgat
    11161 acttataaat gatgatggtg tgattgtcga gactagaaaa atgcaaggtg ttacagtatt
    11221 ggcaagagta aagtcatttg aaggtttggt aggaaagagc ttagagtata tagaaaaatc
    11281 agatttaact gtttacaata aactacttaa aaaagcaata ctagatgcag tcaaacagta
    11341 cataacattt gagaaaaata aatgtgaaga tacaaataat ctgagtactg atgaagagtg
    11401 taggatggaa gtagtttaaa aaaatatatt tataaggagg agttaatatg acgcaatttt
    11461 ttcaagtatt agcaagtggt gcagatagtt ttatgaattt atttagggca ggtgggcaac
    11521 aatttgtagg ttttgtaaca gatatagtac cacttttagt agcattatta gttacaatga
    11581 atgcaattat caactttatt ggtacagata gagtcgagag acttgcaaaa aaatgttctg
    11641 caaatatatt tactagatat ttgatattac cagtccttgg tacatttgta tttgcaaacc
    11701 caatgacact atcattagga agattcttgc cagaaaagtt taaacctagt tactttgcag
    11761 cagcaagctt ttcatgccat acaatgaatg gattattccc acatatcaat ccaggtgaac
    11821 tatttatata tctaggaatt gcaaatggta taacaacttt gggattctca acagcagatt
    11881 tagcagttag atatttgtta gtgggtatag ttgcaaactt tattaaaggt gtagttactg
    11941 actatacaac aaaatttgta gagaaacaac aaggaatagc attaaattca gatataaaga
    12001 ttggataatt actttaaggt ataaataaat ttttcaaata aaaagatagt gtatagagga
    12061 ggataaatta tatggcattt aacaaagtta aaatagtaaa aggttctgga ggatggggtg
    12121 gaccattgat aatagaacca acagaaaaga agaataaggt agtatatgtg actgggggag
    12181 caaagccaga aactgcagta aaaatagctg aacttacagg atgtgagtta atagatggat
    12241 tttcacaggg tgttaaagat gatgaaatcg catgtgtaat aataaattgt ggtggaacat
    12301 tgagatgtgg tatatatcct caaaagaaaa taccgactat aaatataatg aaaacaggaa
    12361 aaagtggacc attagcaatg tttataaaag aagatatata tgtttcaggg gtaaaaccaa
    12421 caaatgtaga gcagttataa ttgaggtagt aggagggaag aaatatggct tatgatacta
    12481 aaaagaagat aagtgagcag agtcaaggaa ttgttgcaaa aataggtata gcactaggaa
    12541 atgtgacaag tactcttttt caagctggta gggatactat agatacatta atacatacta
    12601 tactgccttt tatggctttc gttgcagtcc taataggtat aataaatcaa tcgggtatag
    12661 gaaattgggt agccaattta cttactccat taatcggaaa tgttggaggt ttagttgtct
    12721 tatcaataat atgttcattt ccgttgttgt ctccgtttct tggaccagga gctgttatag
    12781 ctcagatagt gggggtattg gttggtacag aaataggaaa aggaaatatt ccaccaagtt
    12841 tagcattacc agcattgttt gcaataaact cacaagcagc ttgtgacttt ataccagtag
    12901 gtcttggtct tgcagaagct gaacctgata cagttcaagt aggagttccg tcagttttat
    12961 attctagatt tttaacaggt gcaccaactg ttctattagc atggttagca agctttgggt
    13021 tatataaata gaagaatact ttataaataa ttagagagag gttcagttga tatggaatta
    13081 atatacagta ctcaaataaa taaaatagga gataatgcaa atgaattttt acaacataat
    13141 atgtttgtaa ctttcaaaga taatgctcct caagagttag aaaattactg ttatatacat
    13201 tctgaaaata atttagtaaa agaaatactt acggatgatg tgctatcaat aaataatgtt
    13261 gattacaaaa ttacttctgt tggtgagtta gtaaaccaaa atctttcaga attaggtcat
    13321 ataacattta agtttacagg tgaaaaagat ggagcaattg gaggaacctt atatcttgaa
    13381 gaaaaagaaa tagcacctat tggcgtaggt actatactaa aaataattag aaaataaaat
    13441 ttaaaacgaa tctaatcata ttataagtaa attatagata aaagtcaatt taactaatca
    13501 agttaacttt taatgtgata aaaaagggga aatgaaaatg agaaaagtgg ctttagtcgt
    13561 tggaggagga agaagtttag gtgaatattt atctaaacat ttagctaaag taggttatga
    13621 tgttgctatt gcagatttaa atgaagacaa cgcaaaaaaa gtagctgaag aaataaaaac
    13681 tgaatttggt tgtaaatctg taggatatag ttgtaatgcc atagatgaaa gacaggttat
    13741 aagtactgtc aaagctattg aaaatgattt tgatagaata gatttattag tgtacaatgc
    13801 aggagtggct gtaagtaaaa aaataactga ttttggatac aaagatttta aatttattgt
    13861 agacgtaaat ttaaatgggt actttctatt tgcaagagaa gtatctaaag tcatgatagc
    13921 aaaaggtatt gagggtagaa taatacagat aaattcaaaa tcaggtagag tgggaagcaa
    13981 gcataattca ggatattcag caggtaaatt tgctggagta ggacttactc aaagtttagc
    14041 gctagatttg gcagaacata agataactgt aaatgcactg atgctcggta atttacttga
    14101 ttctgatatg tttgaaagtt taattcctca atatgcagta aaattgggaa tacctgaatc
    14161 agaagtcaag caagtttata tagataaagt acctttaaaa agagggtgta gatttataga
    14221 tgttgcaaat atgcttacat tttatgcttc aaaggaagct gaatactgca ctggtcaatc
    14281 tataaatatt actggtggac aagttatgta aggtttttat ggagtgtctc aagatgaact
    14341 ctttagttca tgaggcactt ttttgtataa aattaaatat attttcagca tttaaacaat
    14401 gaaaaagagg tttatcttta ttttgagata gcctcatttt tgtaattaaa aaacatagtg
    14461 tttaagtaaa catataagat tgttagtttt ttaaaagaga attttttaat aatattatat
    14521 ttatgaagta tttttaaact taatttgata aaatatagct atgttttata tactgtagtt
    14581 ttgaacatag aaagtttaat aaattatagt atatatgtta atttttaata aggtaagaaa
    14641 gtagaaagaa ggatatttta tggatataat aaaaaaatta agtcatgggt gtacattaga
    14701 ttgtcatgac tgttgtaaat tcaatgttta tactaaagga aataatgtag taaaaattga
    14761 aggagataaa aatcatcctt atacaaaagg ttttatatgc aaaaaaggga tggcccattt
    14821 agatagatta aatcataaag atagaataaa gactcctatg cttaaagttg atggagtatg
    14881 gaaagagatt tcttttgata aagctataga aataatggca gaaaagctta catactataa
    14941 agaaaagtat acatcaaagt ctgtcatgca ttatgaccaa tatggaagtg ggtctgtgct
    15001 aaaatacata ggagatatat tttttaattt ttatggaggt gttagcagac ataagggtgg
    15061 tccctgttgg agtgcaggga tgcatgctca aaaatatgat tttggagtag ctaagagtca
    15121 tgctatagaa gatatgttaa atagtaaaag tatatttgtt tggggaaaaa atccagcata
    15181 tactacaatt catactatgc aaataattaa aaaagctaaa gaaaaaggga taaagatagt
    15241 agtgattgac cccatatata caaaaacagc tcaaatagca gataagtatg tacaagtaaa
    15301 tcctggaaca gatggagctt tagctatagc tatggcaaaa attatagtgg aagataaatt
    15361 atatgatgaa gaatatataa actcgtatgt tattggtttt gaagaatata aaaaatattt
    15421 atcttcattg gaattaagtt ttttaattga tgaatgtggt gttaaagaga atgatataag
    15481 agagttagtg gatttatata ctaacaaata ttcaagtata aatgttggat atgggcttca
    15541 aaaatataaa aatggtggaa acacaataag agcaatagat gctttaggtg ctataactgg
    15601 tcaaatagga tttagtggtg gaggggtaaa ctatgctaat aaagtttatc cgagtgttat
    15661 aaattctgac ccttacaata gccaatcata tggtgaagat agggagtttt atgtaagtaa
    15721 tatcagtaaa tttatagaag aatctttaaa aaatacatca aataaagtaa attatgcatc
    15781 agatgaactt gatatggcat tacataaagt aaattacata tcagatgaac ttgatacaac
    15841 atcaaataaa gtaaattatg tatcagatga acttgatatg gcattacata aagtaaatta
    15901 tgtgtcagat gaacttgata caacatcaaa taaagtaaat tatgtatcag atgaacttga
    15961 tatggcatta cataaagtaa atggtgtatc agatgaactt gatatggcat tacataaagt
    16021 aaattgtgta tcagatgaac ttgatatgac atcaaataaa gtaaattatg tatcagatga
    16081 acttgatatg acatcaaata aagtaaatta tgtattagat gaacttgata taacatcaaa
    16141 taaaacagat tatatatcaa atgaattgta taatttatct aataagtcaa tcaaagataa
    16201 tattcctata aagatggctg tgataactaa aagtaatatg ctgaatcaac tgccagattt
    16261 agtagaattg gagagggtgt tttcaaaaat tgaatttaaa gtatgttttg atatgtttat
    16321 aactgataca gttactttat gtgacatgtt tataccgtgt acaaatacac tggagagtga
    16381 agatataatt tatagctcaa tgaccaatcc atatataacc tataatgaaa gagctgtaaa
    16441 gccagcacat aaattgatgg atgaatatta cttctttatg gagctagcta aaaagatggg
    16501 attaaatgat tatccttttg tagaaaagag aacttattta gaaaaagtga ttgaaccact
    16561 taagcgtttt gacaaaaatc tggatataga aaaacttaaa aacaattact ttactataca
    16621 taaccctgtt gcttgggaag ataagaagtt tgagactcca tctggaaaat acgagcttta
    16681 ttcagaaagt ataaaaaact tgggaataag tccaacacct gtatatataa gcaataaata
    16741 taaagaaata gaagataaaa atatttcttt tagattatta actaatcatc atgcagatac
    16801 tttatttagt cagcatttta tggacaaaaa atctatagct caagcatata taaatcagag
    16861 aatggctaaa aaagtaggta ttgaggagaa agatattgtg atattgaggt caaaaaaagc
    16921 aaaaataaat gtacaaataa atatagatga tggagtaggt aattatatag taaaaatgta
    16981 tgttggttgg tggaagaaac atggaaatcc taatagttta acagacactg gtatatcaga
    17041 ttttgggggt caggtaacat ataatgaaag tatggtagaa ataatacgac aaaactagtt
    17101 aaatttcatt gaaggaataa aaatataatt atagaattga ttaaaagtta ctatttttac
    17161 aaaactattg aaggagggat ttttgatggt aaattattcc ttagaacata agaatctata
    17221 catagaattt atgtgctcag aactagacca ccatgtagcc aatgaaataa gggaagaaat
    17281 agataactta ttaagtgtaa atcaagtaaa aaatgttgta tttaattttg aaaatattaa
    17341 ttttatggat tcttcaggta taggcgtcat cataggtaga tataaaaaga tttctaatga
    17401 agggggacga gtttcagtaa ttaatataag ttcacgagtt aagaagattt ttgatttatc
    17461 tgggttaaat aaaattattg gcatttatga tacgtatgaa gaagctctaa gttctttgta
    17521 gggaggagta tgtgatgaat aatattatgg aagttaaatt ttcggcaata tctgaaaatg
    17581 aaagtttagc aagagttata gtagcatcct ttgcagctaa gttagaccca acagtagatg
    17641 agttagtaga tataaaaact gctgtctctg aggctgttac gaatgctata atacatggat
    17701 atgaagaaga cagttctaag tttgttttct taagatgtga gatagaagga aatactataa
    17761 agattatagt tgaagatgaa gggtatggaa tagaaaatgt ggagaaggca atggaaccac
    17821 tttatacatc aaaaccagaa cttgatagat caggaatggg attcacagta atgaaaagct
    17881 ttatggacga tgtagaggta agttctgtaa aagataatgg gactagaatt gaaatgacta
    17941 aaaaaataaa tgtgcctaaa taaagtccaa aagtgaggtt ttattatgga agtaactgtt
    18001 gccagagaag aaaaaaaacc tcttcttagt catgaagaga ctctagaact tattgaaaaa
    18061 gttcaaaatg gagatgaaga ggcaaaagaa atattaatat caagcaattt aggtctagtt
    18121 aggagcgttg tatctagatt tttaaatata ggatatgata gagaagattt atttcagtta
    18181 ggttctatag gcttaataaa atcaatatat aaatttgacc caaaatttaa tgtaaaattc
    18241 tctacttatg cagtaccaat gattttaggt gaaataaaga gatacttaag agatgatggt
    18301 atgataaaag tatcaaggtc gttaaagcaa atagctgtaa aggcaaagat ggaaagtgaa
    18361 gctttaacta aaaaattagg tagagagccg agtatagaag aattagctaa agcaatagat
    18421 gtagaaaaag aagatttagt aatggctatg gaagctaatt tcaatgttga gtatttgcaa
    18481 ggagttattc atgaagagga aggttctcca atatgcctta ttgataaaat aagcatgaaa
    18541 ggagaaagtg aagaagaaaa agtagtggat aacatattgt taaaagacat acttggaaga
    18601 ctggataaga gagagcgtca aataatagtt ctcagatact ttgaagacat gactcaaagt
    18661 gaaataggag agatgctcaa tatatcacaa gttcaagtat ctaggataga gaagaaagta
    18721 ttgtctaagt taaaagaata tatatcgtga taatacttat gatatgtaga ataacaaaat
    18781 ataataaata tattactaaa ataaaatgta ataacttttt atgttaagtt tttaaattat
    18841 taaaagatat taaaataata aaaagcatta aaaaagtaaa aatataaaaa caaggtgtct
    18901 ttatggaaaa atagattttt tctttattag gcatcttttt tattttttga ccaaacttta
    18961 gatagtcaaa ataaataata ggtttatttt tttaaaatta acaaataata ctttagagag
    19021 gtgtttagta tggataaaaa ttataaaaaa tatgtagacc aaataagccc aaaacctaca
    19081 tatttaaaaa actatacact tgcatttatt gtaggtggaa ttatttgtat gatagggcaa
    19141 gcaataaatg atttgtatat gaaagttgga ggtcttgata aactaggtgc tagttctgca
    19201 acatcaataa cattaatatt tataggggca tttttaactg gattaggagt ttacgaccta
    19261 ataggaaaaa gagctggagc tggttctata attccaataa caggttttgc taactcaata
    19321 gtatctccag caatggagta taaaagagaa ggctatgtat taggtgtagg tgcaaactta
    19381 tttaaaattg caggaccagt tttagtttat ggtataggtt cttctatttt atgtggaatt
    19441 atttattata tttttaagat gttttagatg atttttattt aatttaaatt ttattaatta
    19501 tgaagaaggt ataaaatctg aaaatagcta ctaaagtaac taattgaaaa ataattgtgc
    19561 agatttaatt aaaattttta attaatatta aaaatttgaa tagtaaaatg caaaattaaa
    19621 aaggtgtgaa aatatgaaaa ataaaagaat tggaaaaaga acagtcaagt tagaaaataa
    19681 accaaccata atatcaactg gaactatagt aggtcctaag gaaggtgaag gaccactaaa
    19741 ggactatttt gatatgatta tgactgacga tttatatggt gaaaagactt gggaattagc
    19801 tgaaagtaaa atggtagaga ctgcatctca acaagctatc caaaaggcag gtaaaaaatt
    19861 gtcagatgta aattatatgc taggtggcga tttaataaat caaatagttc cagcatcatt
    19921 tgcagcaaga gaactggcta ttccattttt aggtatatat ggagcttgtt ctacaatggc
    19981 agaaggtctt tgtataggtt ctatgcttat agatggtggt tttgctgatt tagtactgtc
    20041 aggtacatca agtcactatt gtacagcaga aagacaattt agattcccac ttgaacttgg
    20101 aaatcaaaaa ccaatgacag cccagtggac agttacagga gcaggaagtg tattattagc
    20161 tcctaatgga gacggtccaa aagtaaagta tgttactgtt ggtaaagtaa tagatgaggg
    20221 tattgatgat ggtaataata tgggaccagc tatggctcca gcagcaatag atactatcta
    20281 ctcttacttt gaagatacta atgatgaccc aaatagcttt gatataattg ctactggaga
    20341 tttaggtact cttggaaaac agatagcaga agactttcta aaagaaaaag gtgtagatat
    20401 atcaaaagtt tatactgact gtggtataga gatgtttaac cttaaagaac aagatgtcca
    20461 ctgtggtgga agtggatgtg ggtgttcagc cacagtgttt gcaggatata tatatgataa
    20521 gttgagaaaa aaagaattta acaaagtaat gcttgtatca acaggagcac tactttcacc
    20581 gacaagtaca ctacaaaagc agacaatacc atgtgttgca catgcagtag taatagtaaa
    20641 tgagtaggaa gtgatataat atgttaatgg actatgtaag agtatttatt gtaggtggaa
    20701 ttatatgttt aatagcccaa gtaatgatgg attatttcaa aatgcaaact ccatatgtta
    20761 tggtaacgta tgtaacaact ggtgtagttt taacttttct aggactttat gagccattag
    20821 ttaagtttgc agcagcaggt gcaacagttc ctataatagg atttggatat tcacttgcta
    20881 caggagtaat aaaatcaatt caaagtgatg gttttttagg tattttttta ggaggtacaa
    20941 ctgctacagc tggtggaata gcagcagcta tattctttgg atatattatg tcagtagtat
    21001 ttactccaaa agcaaaacca taaatcttta ttaaaagaat aataagtttt tgaaataata
    21061 aatttttata aagcaggtgg atatctttta gattccacct gttttttata taaagaagcg
    21121 tttacataat tgaagttgac aattatttac aacatagtgt agtattatat tatcaaaata
    21181 aaatgaagtg aagtagggga taaagatggt aactattaaa gatattgcaa ggaatctagg
    21241 tatcagttat tcaactgttt ctaggtgctt aaacaataat cctaatgtat ctgaaaaaac
    21301 aaaaaagaaa gtggtagaag aggcaaatag actagggttc cattttaatg taaatgcaag
    21361 aaatttagca aaaaaagaaa ctaacaggat aggggtaatc ttttcaaata attttaatca
    21421 tcaagatact agaaagtttt ttagtgatat aatggatagt tctataaatt ctattgagac
    21481 taataaatac gattttataa tacagcctaa taataacatt agtggtgata gtaatgtata
    21541 taaaatggta aatggacaaa tggttgacgg attagtaatt gtgtctcaat caataaaaaa
    21601 agaagagtat gattttttaa aagacaataa ttttccacat gtatttatat attttaaacc
    21661 atcttttata ggagaagtag ataatttctt ttgggacgat aacgtgtatg gaggttatat
    21721 agctaccaag catttaattg accatggtca taaagatata ataacagtaa cttcagatga
    21781 taagagttca aaaatgcatg aagatagaac caagggatat ttgaatgcta tggaagaagc
    21841 aaatttaaag acagaagtca ttaaatctaa aatggatttt gaatctcaag ttgaattttt
    21901 agaaaaatac attgataaaa taaaaaaagc ttcagctata tttgtacaac aagatgtacc
    21961 agcgatttct ataatacaag agttaaaaac tacttatggt ataaatgtac cagaagatat
    22021 ttccataata gggtataata atattgaatt aatatcatat tttaggtcgc atttatcaac
    22081 aatagatgac ccaagagagc aagttataaa aaatggagta gatagtttag taaatataat
    22141 aaataaaaag agtacagaac acattccgag aaaattatat ccaaggttaa taatacgaaa
    22201 tagcgttaaa agaataaagt agtaagcttt taagtatcaa acgtttatta aaaatattat
    22261 agattatgtg aagtttttta acaaataata taaataaaaa ttaaaaggag taggtgtata
    22321 taagtacatt gtactccttt atttattgag atagttttta taatatgaat gaataatttt
    22381 attcaattat ttgaaaatcc gttattttaa caatataaat taataaatta tttgtatttt
    22441 atatcatgaa tctataatat ttttttgatt cataactaaa agtatataaa aatgatgaat
    22501 aaatatatat tatacattat caatgatagg ttatataata aaatcactaa caattatatt
    22561 ataagaaaat tataaaaatt acaaggaggt atagaaatga aagaaaaagc agggattaga
    22621 aatgccataa tagcagcctt tataatcaca atattggcag aattaattgg acctatttca
    22681 tttaaagtta tgggaattaa tgtaacttta cttagtatac tatgggctat attcatagga
    22741 atggctgttt ctcctcattt attagggaga gttataccag ccttaaaaaa gtttataggg
    22801 gataatgaaa taaatgtatc tccatattta ttatctttga cactatatcc attagggata
    22861 atgtttggaa ttaatgcagg gcctaaagtt ggtatattac ttcaagcagg accagcactt
    22921 ttattacaag aatttggtca tatgggtaca atgttgatag cattacctgt tggagtagca
    22981 ttaggtcttg gaagaagtgc attaggtggt actttttcat tatgtcgtga cacagcacta
    23041 ggaattattg gagataagta tggattaaac tcaccagaag gtataggaac actaggaact
    23101 tacataagtg gtagtatatt tggaacatta ttattttcat ttttagcacc agtgggtgta
    23161 cttttaggat ttcatccata tgctttagca atggcatcag gaatgggtag tggttcaatg
    23221 atgggagctg caacagcatc attaatgaat acggttccac aaatgtcaga gcagatttta
    23281 gcatattcag ctactagtgg attgattaca gctgttactg gaatatatat agaattattc
    23341 ttatctttac cagtagccaa ctactattat agcaaagtag ctccagttat tgaaagattt
    23401 agaggaagaa aaacaagaag aacaagtaat aaattgtaaa ggaggatata tatatggaaa
    23461 aaacttttaa gactattatg gagcagataa aaatagtatt gtttatatat attattattt
    23521 gctttggaca aatgatagca cttaagactc aggctcaaga tttgttgatt ggaagtgtat
    23581 ctggattttt gttggtgata attgctatta taataaagaa ttttgttaaa aaacctaatt
    23641 taccaggttt tgcatgggct acattagttg catttgtttt atcgcttcca atatctccag
    23701 tgggggatat catagcaaca aatgtaggga aaatcaattt tatgtctaca gtaacaccat
    23761 tattagcttt tgcaggaatc tctgtaggta accaattaga tatattaaag actttgagtt
    23821 ggaagttagt agttatatca tttatagtta tgacatctac ttactttggt tctgctttta
    23881 tatctcaaac agttttaaga ttaaatggaa tgctttaagg aggtagtaaa tatgaatgaa
    23941 ttaaaagcaa aaattaaaga ggaaataaag ttattatctg aagagaaaaa gacatcattt
    24001 gaaaaagttt ctgactattt attttcaaat ccagaattag catttgagga gtataagtca
    24061 caaaaagcat tatgtgattt gttagaagaa aatgaattta atgtaactaa aggtgtagga
    24121 ggattagaaa catcttttga agctgtgtat tcaaatggaa ctaatgggaa aacagttgct
    24181 tttttagctg aatatgatgc acttccagga atgggacatg catgtgggca caatatcata
    24241 gggacaagtt ctgttggtgc aggtatcata ttaaaagaaa tcatgaaaaa acataatata
    24301 gaagggactg taaaggtttt tggaacacct gcagaagaaa gagtaggtgg aaaaataact
    24361 atgattaaag aaggtgtatt taataatgta gatgctgcat taattttaca tccaagtgat
    24421 gcttcaatgc ctgatgatat atcatttgct caagttaatt taaaatttga ttttactggt
    24481 aaggcatcac atgctgctgc ttttccttgg gaagggaaaa gtgccttaag tggagtaata
    24541 gcactattta acagtgtaaa tagcatgaga ttacatctta aagattatgc aagagtacat
    24601 ggaattataa ctgatggagg tagcatacat aatataattc ctgaaaaatc tacagctata
    24661 tttaatgtaa gagctttaag catagaatat ttaaatgaga tttgtgaaat gcttaaaaat
    24721 tgtgctaaag gagctgcaat ttctactgga actaatgtag aaatagttca attagatgaa
    24781 atatacaaag agattaagaa tgattctgaa ttagtgaata tagttagaca aaattttgaa
    24841 gttttgggtg aagactatgt tgaaagagat ttaagtcaag gtataggttc aactgataca
    24901 ggaaatttaa ctcatgagat acctgcaata caagcatata taaaattaaa agaaaatact
    24961 gcaactcata cagatgaatt tgctgttgca gctggtggtg aagaaggcag agtagcactt
    25021 ataaaagcaa ttaaggtgtt agcaatgtgt ggagtagata ttttgtatag taaataatat
    25081 ataattttaa aatatattaa gaaaggaaat cggattagac atgatattaa aacaagtaat
    25141 agaggtttat gacattttag ataaagcaaa tgctaatggg gaagaagtaa aaacttactt
    25201 acaaggttat ggtgaagtag atgtaactgt aaaagaattg tcttcaagta aaggctcaac
    25261 agatttagta aagcttacaa ttcctgggaa aaatggaaaa ttaaaaggtg gagatgctcc
    25321 tactcttggc attttaggta gactaggagg gattggagca agaccagaag ttataggttt
    25381 tgtttcagat ggtgatggag cattggtagc aattgcagtg gctgctaaac ttttggatat
    25441 gcaacgaaaa ggggatatat tagatggtga tgttgtaatt tcaacccata tttgcccaga
    25501 tgctccaact aaagaacact atcctactcc ttttatggat tctccaattg atatgatgac
    25561 tatgaatgaa aatgaagttg atagtagttg tgatgcaata ctttctatag atactactaa
    25621 aggaaataga gtaatcaata ctagaggatt tgcaatatcg ccaactgtta aagaaggcta
    25681 catattaaaa actagtgata accttttaga tattatgcag actgtaacag ggaaatctcc
    25741 atttgtattc cctttgtcta ttcaagatat aacaccatat ggaaataatc tgtatcactt
    25801 aaatagtatt ttacaaccag cagtagctac agatgcacca atagtaggag tagcaattac
    25861 aacagagatg cctgttgctg gatgtgctac aggtgcaact aattttagtg atttagattc
    25921 agctgggaga tttgcaattg aagttgccaa gctatttggt agaaataagt gtgatttcta
    25981 tgataaagaa gagtgggaaa tgcttattaa gagatatggc aaattaaata gatttcaaac
    26041 atttggtata cagtaattag aaaaatatta agtttatata atttgtgata taactattta
    26101 agaatatata aacaattagg aataaaagta agaataaagc acttagttaa aaactttaaa
    26161 aataaaatat ttatttgttt atataaaaag gaggattctg gttattggga ttctcttttt
    26221 tatgaataca aataatatat tgtaattata aaaaaaggag cgtttcaaag tgattttttt
    26281 aatcaccttg agacaactcc ttatattttt atttaaattc tattttttat taagttgctg
    26341 gagttccacc accatcagga ggagtagtag agccaccacc attatttcca ccattatcag
    26401 gaggagtagt agagccacca ccattgtttc cgccattatc aggaggagta gtagagccac
    26461 caccattatt tccgccatta tcaggaggag tagtagagcc accaccattg tttccgccat
    26521 tatcaggagg tgtagaaccg ccaccattat cacctgtgtt attattgtta ttattatttt
    26581 cttctggttg gttattgtct ggtttttttg tatcttcaga tgatggttga gaacttactt
    26641 tagtaggagc agtaccttca ataaatgctg ctttagagcc agaagattgc tttcctccat
    26701 cctctaaatt aatttttgta aaatatacat ttttaggaac ttcaaattca gtaaccgtta
    26761 aattagcatg tattttttcc ataatttttt cccatagttt tgcagtacta gtacttccat
    26821 gtgggacttc tctacgtttt atagtgttac cagaatcatc ttttctacca gcatcatctc
    26881 caatataagt tgcaccaaca taataaggag tgtaaccgac aaaccaagca tcatatgcac
    26941 tatttgtagt acctgtttta cctgctacag gcattccctt tggaagtgta gcaacaccac
    27001 cagtaccttc agtaattacg ctttctaaca tatctgtaac aacataagct acttcagggt
    27061 ctacaacttt atgttcttca ggagcatttt taactaataa ttgaccatca aaggtagtta
    27121 tagtagtaaa aatagaaggt tctacataaa taccgtgatt tgccaatgta ccataagcag
    27181 cagccatttg aagtggagta ataccattcg ccataccacc tagtgtcaat gatgggaatt
    27241 ttctatcact tgcttcagat gaacctgagt gattattctt taaagttgtt ataccaaagt
    27301 tctttaagta atccatcatt atatctacac attcatcata agagtctcca agcatttcag
    27361 cagttttgac agctatcgtg tttgaagatt tagcaagtgc tttacgtaaa ctcatagaac
    27421 catgaccagc agtagtagtt cttggattcc attcattatt ttctttaaac ttatatcctc
    27481 ctcgagaatc acttagagca gttgcttgtg ttatttttag agtatcaata gctggcgtat
    27541 atattgctaa aggttttata gaagaacctg gctgatgagg gttagtagca cgatttaaag
    27601 tttttctacc acttatattt cttcctccag ctagtgctct tatttcacca tttttataat
    27661 ctaagattac cattgcagct tgcggttggc taactccatc aggaccgacc atatgtcctg
    27721 ggaaattgct atttctatca aattcttctt ctaaagcatc ttgcatttta gggtctatag
    27781 ttgtgtttac aattagacca ccattgtaga ataagttttg agcttcatca tttgtatgtc
    27841 cttgttccat taaagcattt ataacttcac tttcgataag gtcttctaca gatgaagaaa
    27901 ctttatctga tgattgaggc aattttattt ctattttagc tttgattgct tcatcatatt
    27961 cactttgttt tatatatttc aattctaaca ttttctttaa aacaatttct tgtctttttt
    28021 tagcttcagg attacttaca gcctttctaa caactaatgt acctgctttt aattgtctat
    28081 aagtatcatt agatataagc tcccaagatt ttattttttc aatcatatca aagtccactt
    28141 gagtagggtc atctaaatca tcatcagttg tgttaacaaa aaataataat ttattctcta
    28201 aatcctcctt agtttcattt ccctctaact tagaagtttt ataggctgaa aatcttgatg
    28261 gatttttagt tgaaccagca agaagtgctg actctgctag agtaaggtca gctgcagact
    28321 tatcaaaata accacgtgca ccagcctcag cacctgctag acctctacca acgaagaagt
    28381 tatttaaata agtttctagt attttatctt tacttactgt tttactcatc tcatgtgcat
    28441 agtaaatatc ctttatttta cgaggtatag tttgctgttc agttgttaac agcattttgg
    28501 atacctgcat aggaatagta ctaccacctt gcttagtacc tgtaagggtc tttaatactg
    28561 acctaaataa acctttaata tcgacaccct tatgctcata aaaacgctca tcttctattg
    28621 caattacagc atgttgaaga taaggggaaa tcttatctaa tggaacgggt gtctttttat
    28681 ttacacttgg agctgtagat aatgtttttc cattagcgta tttaatagta gttgtttggt
    28741 tcgtctgttt atctaaaaca gcttctgtaa ctggagttac atctcttagt gacgcaaata
    28801 ctagaccagc agtacctgct gtaccaacaa ctagtaaaac taaaaatact atcccaaata
    28861 ctcttagttt tttaaattta tcgctttttt tagatttttt cttgtttttt gttgaacgag
    28921 tcttgttagc tgaagttcta ttaattggtt tatttgtatt accagatgag ctaacttttt
    28981 ttctcctgat tttatttcca tttttatcat tatcgttatt cataagattt taacctcctt
    29041 acttaatgca atattataac acaaatagga gtagttttgt aattaataga aaaaatgaat
    29101 ttgctataat attctgtaca ttgtatatgt ataaacgtaa tatattaaaa agtctatatt
    29161 tttattttat ttatttttga caaatattat aaaaattatg ccaaagctac atataaataa
    29221 ataggagggg ataattttga atagaatagt agatagaaaa acaaaaaatg aatttagaaa
    29281 aatcatagct atttttttag taattctttt cttgtctgta tttataggca gttttatata
    29341 tatagataaa actttaagac cgacaataac agttttagca gagactaaag ctcttgaact
    29401 agcaaatagg tcgataaata aagctgttgc tgagatggta gaaggaaaaa taaattatga
    29461 agatttaatg gatatacaac ttgataataa tggtaaaata actatgatac aagcaaacac
    29521 tataatgatg aatgaaattg catctgcaat agctttagaa atacaagatg aattaaaaaa
    29581 ggataagaca gcaagttctt acatacctat aggaacagca ttaggaagtc ctatattagc
    29641 taaatacggt cctaaacttg aagtatctat tgaacctata ggaacagtat cagtaaactt
    29701 taaaacggaa tttgagtctt caggtataaa tcaaactaga catagaatat atttagaagc
    29761 tcaaacacaa gttaaagtag taataccact tataacttca acaaaacaaa ttaaagctca
    29821 aatacctatt tgtgaaacta taatagtagg tgatgttcca gaaagttatg taaatattcc
    29881 agaaaaaaat ctaggaaatg ttttacctaa cactggaaag aataccaaca agtaatataa
    29941 agttgtatat ttttaaataa attcaaaata atagtttata aataaaattt ttggaaataa
    30001 tattaatata attttaataa aaatttatat tttatactat gtaaaatatc taatatattt
    30061 ggtctgtagg ttaaataaaa gtacctttag accaaatata ttattaataa cttaataaca
    30121 tcttaaaact tatttattta tttatattat ttacaacatc catttaataa atacaccata
    30181 acctacagct gggtcgtttt caattgcatg tgacactgct ccgattattt tattatcttg
    30241 aactattgga gtaccactca taccttgtac aataccacca gtttgagcta aaagcttttt
    30301 atctgtaatt ttaattttaa aacttttact ttctggatgt ttttggtttt ctatatctat
    30361 aatttcaata ttatattttt tacacttatt aaagttatct tgtaaaatta tttgtgcttt
    30421 tcctaattta acttcatcta aagatgctac tttatatttt tttaacttgg aaatatcaaa
    30481 gttattaatt tgacctctta ttccaaaatc agtattttct gtaaagctac caataatatt
    30541 ttctcgtttt gcatttatac atccaacact acctttataa gatttttcta tattaaggtg
    30601 cttagttgta gaaattgaac ctgttttaat aggtattttt cttgcattac caacacttat
    30661 tggatgacct acagctccaa actctgatgt atctggattg atatacgtta aagtagcaaa
    30721 tccagaaagt aaattgttaa aactaatttt ttctaatatg tgtttggtag ttttcattgt
    30781 tataagttgt ccacctctat ttaataaaat agatacttgt ttttcttctg gtaaagcatt
    30841 taaggtattg gcaaagtcac tatatccatc aacttcagca ttatttacct ttactatagc
    30901 atcacccaat ctaaatggag atgcttctcc aggatttcta actatgatat tttttaattc
    30961 tgcgtcaatt tgaagtacgt ttccaattgg tattaaatat tcaggttcat ttttttgtaa
    31021 attttgtgca taaattaaat tatttgagaa aaaatataat aaaaataaaa ataaaaatgt
    31081 taaaacaatt gtaaattttg acttagctaa gttggttttt ttacttaaag tattattttt
    31141 aaaatgaaaa tttttaagtt gcataaaaca cctctttctt attaaattat tttaataata
    31201 gaattgaagt tttcttgctg cttctatatt atgttgtgca agaatcatgt tttgatgctt
    31261 ggaaaactag ctcaataaca ccttagtttt cataaaaata aataaaataa atagtgtata
    31321 tgataaaata aatacatatt catataaata aaaataaaca ataactgtct agcataagaa
    31381 aggagcaaaa aatgaaaaaa tatagattgt tagttacttg tataattgcc atgggaatca
    31441 taattatagg aacatcaaat ccagttagca atttttacaa gactacaaat ttgattagtt
    31501 caggaaatat cacaaatgaa aaagcaaata aagatgaaaa ttcaaacaaa aataataaag
    31561 ataaaaatga agacaaatct aattctaaaa gtaataaaga taagtcatca gaggacaaat
    31621 cgtcagataa taaaaatgaa attcaaaaaa ataaaaaact tttaatttgt atagacccag
    31681 ggcatcaagg aaaaggagat agcaatttag aaccagtagc tccaggttct tcctctaaaa
    31741 aagcaagagt atcttcagga acagagggta ttgctacaaa aaagccagaa tatgttttaa
    31801 acttagaagc atctcttgtt ttaaagagta tattagaatc taaaggttat aatgtaataa
    31861 tgactagaga gacacatgat gtaaatataa gtaattcaga aagagcaata cttgctaatg
    31921 acaaaaaagc agatatggtt gtaagaatac atgcagatag tttaaataat tcttcaaaaa
    31981 ctggagcatc tatattgatt cctgaaaaag atggaaaata tacagctcct atatatgaag
    32041 aaagtaataa atgtgctgaa tttatcaaac agaatatgga acaatcaggt atacaaatca
    32101 atggaatcgt tcaaagaggt gatttgactg gattcaactg gtcaaaagta ccagctgtat
    32161 tagtagaaat gggatttatg agtaactata atgaagacca aatgatgtca aatccagact
    32221 accaaagaaa aatgatgcag tgtatagctg atggattgga tgcgtatttt aaataagata
    32281 tgaatagaga aaaagttagg ttggtattat tttcctaatt tttatctcta ttttttattg
    32341 tacataacac aaagaagaag tttattttag gttaaattgt cgtaaatata actaaaaata
    32401 ttgggaatac ttgatttaag tttaaaaaga atatattata gtatagaatg tgtaaaattt
    32461 ttttgagagg tgttgaaatg ctagtagtag aaaatgtgag tcatggattt ggtgcgagaa
    32521 caatattaga aaatgtatcc tttagactta gaaaaggtga acatattgca ctagttggtg
    32581 ctaatggaga aggaaaatcg agttttttaa atataataac aaagaagctt atgccagatg
    32641 ctggaaatat aaaatggtct tcaagagcta cagtaggtta cttagaccaa catacggtat
    32701 taagcaaggg aaaaactata agagaagtgc ttagagaggc ttttaagcat atgtttgact
    32761 tagagcaaga aatgattgct atgtatgaca aaatgggaga agctagtgat gatgaaatga
    32821 gtaagcttct tgaagaaaca gcagagatac aaactatact tgaaaatagt ggtttttata
    32881 tgatagatgc aaagatacaa gaagttgcaa atggtcttgg tcttggagaa attggtcttg
    32941 ataaagatgt aactgactta agtggagggc aaagaacaaa agtacttctt actaaacttc
    33001 tacttgaaaa tccaactatt cttatattgg atgagcctac aaactattta gacgaagagc
    33061 atataacatg gcttactaag taccttcaag agtatgagaa tagttttgtc ttagtttctc
    33121 atgatattga atttataaat aatacatgta atgtaatata tcatatggaa aatggagagt
    33181 taaatagata taaaggaaat tatgatgaat ttgtaagact taatgatata aagaaacgtc
    33241 aagaagaaca agcttatgat aaacaagttg aagaaagaaa aagactagag gattttgtag
    33301 ctagaaataa agctagagta gcaactagag gaatggctaa cagtagacaa aagcaacttg
    33361 ataagatgga aatattagaa agaccaaaag aaaaaataaa acctactttt gcttttaaag
    33421 atgctagagc agctagtaaa ataatttttg aaacagaaaa tttagtttta gggtatgatg
    33481 aagcattgac aaaaccactt aatttccatt tagaaagagg aaaaaagatt gccttaaaag
    33541 gaatgaatgg tattggaaaa tctactctat taaaaacttt acttggaata ataaaaccat
    33601 ttgaaggaaa tgttaaatta ggtgactatt tggaagttgg atattttgaa caagagagtt
    33661 caagagaaaa tagtaataca cctatggatg aagtttggag tgaatttcca gggcttacaa
    33721 actttgaagt tagacaggct cttgcaaaat gtggacttac aaatgaacat ataacgagtc
    33781 aaatgagagt attaagtggt ggagaagctg ctaaggttag actttgcaaa gtaatgttaa
    33841 aaaatataaa tttcttagta ttagatgagc ctacaaatca tttagatgtt gaggcaaaag
    33901 atgaattgaa gaaggctatc aaagagttta agggaacagt tcttttagta tgtcatgagc
    33961 cagaatttta ttcagaaatt gttgatgatg tatggaatat agaagatttt acaactaaaa
    34021 tcgtgtagtg atttaactta ttgctggggg gatttcatga aaaagtttct aaatgtactt
    34081 ttttcaattt gtattagtat tgttataata gtaggtgtta ttaattttac tgttggattt
    34141 aaacaacttt attattttga cattgattac ttaaatatat cagagttatc tggtttatct
    34201 aaagatgata taaaattgaa ctacgattat ctaattgatt ataacttaaa taagaatgtg
    34261 tctgagttca aattaccaac attaaaatct tctccacaag ggaagataca ttttgaagaa
    34321 gtcaggaata tatttcaaaa tataaataaa ttagctaaat tattattggt agtttcttta
    34381 gtaggaatta tattgagtgt aaaaaataaa aatataaaaa tcttaaaaac tacatcaata
    34441 acacttataa ttatgccatt attattaaca gttccaattt tattaaattt tgaaaagtca
    34501 tttattattt ttcataaatt attatttaga aatgattatt ggatatttaa tc
//
LOCUS       DAEPIN010000045        33143 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.045, whole genome shotgun sequence.
ACCESSION   DAEPIN010000045 DAEPIN010000000
VERSION     DAEPIN010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 33143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33143
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.045"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            complement(75..917)
                     /locus_tag="KR250_002306"
     CDS             complement(75..917)
                     /locus_tag="KR250_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBH0068237.1"
                     /translation="MINIGDFNKLTVKRKTEFGYFLDGQTNNTKDDILLHNRLIGKNE
                     INIGDEVNAFIFKDSDDRTAATLIPPLAKVGDVAHLKVVDNTDIGTFIDMGLPKDILV
                     PFKAKTYPLFRDEKYLFYIYLDKSKRIAATTDIDSYLLTDHTYNVGDIVTGVVYGFQT
                     NNSAMICVDNKYAGVILHNEYFTELKTGDVLENLHVIKIYEDGKLGLSPRGNRKDELD
                     TLENKILSYLEGSDGYMRFNDKSDPKDISILFNSSKKNFKRALGVLMKKGLIYQDEEG
                     TYLK"
     gene            1212..1946
                     /locus_tag="KR250_002307"
     CDS             1212..1946
                     /locus_tag="KR250_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBH0068238.1"
                     /translation="MKIVNKSSNIPLHTQLSSIIREMIETGELKEGDAIMPERELCNI
                     QNVSRMTVNKTIVGLVTEGLLYRVQGKGTFVAKQKKKYQFSNVKGFTDVMKEKGVNIK
                     TDILSFEMELPDDLVKRKLGISDNTTNIYKIVRLRYTDGEPFGLEIVYLSEEMCKGLT
                     KGILDNSSLYRVLNEKYGYKIQKAEQVMEPVILSDEESKLLETDEGALALKLHRNSYN
                     REGRPIEYTISIFRTDKYQYEIVLSE"
     gene            1956..3089
                     /gene="nagA"
                     /locus_tag="KR250_002308"
     CDS             1956..3089
                     /gene="nagA"
                     /locus_tag="KR250_002308"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HBH0068239.1"
                     /translation="MKCLINGKIILKNQILENKVLVFDEKIIDIADSVPKDCEVIDAD
                     GKYISPGLIDIHIHGNMGKDTMDSTDESIETISKSIMRHGVTSFLPTTMTMDKEHVYD
                     ALEVIKKAQNRKLEGAQVLGAHLEGPFINENYKGAQNEKFIINSKYEFIKEYKDVIKV
                     ITYAPEKDIDFDFTREIKRCTDIVLSIGHSNANYDQAKEAINLGVTNVTHMFNAMTGL
                     NHRDPGVVGAALTTNVYSELIADTIHINKDLFQFILNNKGKERLILITDSIEAGGLED
                     GNYSLGGQKVIVKGNEARLENGALAGSVLSLNKMVFNFLNNTNLKVNEAINLASLNPA
                     TSLGINDKKGSLEIGKDADIAVFDENLDCKMTLCLGEVVYKNI"
     gene            3188..3937
                     /gene="nagB"
                     /locus_tag="KR250_002309"
     CDS             3188..3937
                     /gene="nagB"
                     /locus_tag="KR250_002309"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012425552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HBH0068240.1"
                     /translation="MRVLVCKDYDGMSKKAAEMIAAQIVLKPNSILGLATGSTPVGMY
                     RDLVKKYNDNIVDFSDVMSFNLDEYYKLPISNDQSYDYFMKENLFNHVNIKPENTHLP
                     NGMADDIEKECMNYEASIDAAGGIDVQVLGIGRNAHIGFNEPDTKFAKRTHVVELTES
                     TIEANARFFKSREDVPKKAVSMGIGSILKSKKILLLASGEEKADAVYNTVYGDITPEV
                     PGSILQLHKDTIVIVDEAAASKLNPKDYKLV"
     gene            4089..4361
                     /locus_tag="KR250_002310"
     CDS             4089..4361
                     /locus_tag="KR250_002310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0068241.1"
                     /translation="MLKIIMFSGILSSIIKLPIIGILPTGNIKKNTGISDSKKDGKQR
                     RSNLGISIYQLPSFLYSLINNSVPSNKPIPPEEIKLIKMYLLKSCI"
     gene            5021..5725
                     /locus_tag="KR250_002311"
     CDS             5021..5725
                     /locus_tag="KR250_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0068242.1"
                     /translation="MSKLIYIADDEDNIRNLVKTFLKNEGHDVMDFKTGDELLEQFNI
                     KECDLVILDIMMPGSSGFEVCTKLREKSTVPIIMLTARDTDIDYITGITLGSDDYFTK
                     PFSPMSLVMRVKSIFRRIEFEKKQNYDKYSNSIDMELKFGDVIINKKNKIVTSKNVNI
                     DLTPNEYNLLTYLFENIDRAISRDELLNKIWGYDIEVETRAADDTVKRLRKKILDTNI
                     LIETVWGFGFRLKEKS"
     gene            5727..7214
                     /locus_tag="KR250_002312"
     CDS             5727..7214
                     /locus_tag="KR250_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBH0068243.1"
                     /translation="MKNGNDKNRIGKIKSKFKLKFSKKNISIKTRILLSILCLIIIVF
                     MIILVSFNVFLDTYIKTTANEELTKSTEIVEHMDSNFRPIKPPQDDGKLPPKVLSNFM
                     RNVQDKVRMAETQSDADAMVVDSKYNLIFPTREDDFLKNVDEMELIRECIQNEKLDLN
                     SDANTRISTRNKDYYISTVKITSIYGEQDKYLILFIDISKTLNLAQKINIVLISVMCF
                     AGILAIFTAVILSEKIAKPIKELCEFAKKMGQGDFKRTYFDFSDKELVELSTVMNKSA
                     EYLDKYDNEQKIFFQNASHELRTPLMSIKGYAEAIKYNVIDSKHASDIILEESDRLSD
                     MVEDLLYISKIDNITKDFELVECDLREVLSNCGARQNVRAINKGIKFIYEFDEEMVLF
                     ECDEKNISKAFMNLIENALRYAKSEIKIVCKYNQKNIVVIIEDDGIGIKKEDLPHVFE
                     RFYKGVGGNHGIGLSIVKSIVNKHGGRIYVENGKKGAKFTILFKL"
     gene            7419..9032
                     /locus_tag="KR250_002313"
     CDS             7419..9032
                     /locus_tag="KR250_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBH0068244.1"
                     /translation="MKEQLIFNITNLVTVVFEAFVIHMFLSDFLGQKEGYIKVVRYAK
                     LGFIICLGFCNLITLNPKITMPLIFILIFSTSFLYKGNLKTRLFTTVLLSIFFILSEI
                     VVTSIFVLFVKEGFEIMLENNSIRVLATILSKIVFLLTCKIICLFKKDVHLDMPIKYW
                     LPLFLIPIFSLFLSVSIFDVSKFFSLESLKFLSLISSVGILYINFIVFYLFKFIIDKT
                     KLSMKYELLEKEIIHKEELRLSNECYKIIVEQTDSVVFEWNIKENKSFVSQAWTEKFG
                     YNNACKNIFKEIKDKDLVHSEDKAIFEGFLESIKKKNMHNQAVYRLKKSNGEYIWCRT
                     SITSIYNDENELLRVVGVIVDVDSDIKKYEELRTRAESDSLTNIYNKGTFEKLVEETI
                     VMNTGDKKDALFIIDLDDFKEINDNFGHPFGDFVLKTFADKIQTSFGSKDLVGRIGGD
                     EFVVYMQDYVTEVNLHKKAKELNRVLSDNYTDLSFSFDASVSIGIARYPQDGTSFFEL
                     FKNADRALYSIKASGKNSYCLFEEELYVQ"
     gene            complement(9395..10225)
                     /locus_tag="KR250_002314"
     CDS             complement(9395..10225)
                     /locus_tag="KR250_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBH0068245.1"
                     /translation="MSQVSKNYFTTGEFAKICGINKKTLFHYDDIGLFSPELKKENGY
                     RYYSYHQLSIFGIISSLREVKMPLKEIKAYIDKRTPNLLIELLEKKTIDIKNEIEKLN
                     NIQALMESTISFTKNACNIDANTITLKEHEEEYLVKTPVTYKEQFLGDEEENFLYECI
                     NFMDNYELSDYGTIGSIIKGEDIINKDFESYSYLFTKVNKEYKKYPVSIKPKGLYVTA
                     YHKGSYETIYKAYEKLLNFFNQNNLRIGDFVYEEYLLYDISVRDSNEYLTQISAEVKM
                     "
     gene            10298..12025
                     /locus_tag="KR250_002315"
     CDS             10298..12025
                     /locus_tag="KR250_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0068246.1"
                     /translation="MKKLIHFLKPYRVLIVVVLIFTFLQTLGTLYIPTLTANIVNNGV
                     VKGDIDYIVKTGLMMMIVAGITALSAVLVCKVSANLSSGFCRDIREAVFIKSQDLSIN
                     DFNNIGTASMITRSTSDITLIGQSVFMFIQLVLPAPIITVSGLFLAYSIDKAMTIIIV
                     VVMFLFMLSAFLVGKKLIKLFKMMQIKMDNMNRVLREVVTGVRVIRAFNRSHFEKKRF
                     DRTAIDYSETAISINKIFAVLMPIVMLIMNLGIVSIIWFGGMRVSNGNMEIGHIMALV
                     EYCILILFYLIMGVMVFMYIPRAGACADRVNQILDIEPEIVDGNGHKDTVSERGHLVF
                     KNVTFSYAQSEEPVLNNITFEAKSGEVTAIIGSTGSGKSTIANIIPRFFEIQSGEISI
                     NGQDIKKIPQKELRDKIGFVPQKAFLFSGTIEENIRYGKEDASIEEVKHAASIAQADE
                     FISDMEDKYDSFVAQGGNNLSGGQKQRISIARALVRKPEVYVFDDSFSALDFKTDKRL
                     RKALKNEIKDSSAIIIAQRISTIMDANQIIVLNDGKIVGIGKHKDLLENCEVYKQIAD
                     SQLSKEELA"
     gene            12062..13924
                     /locus_tag="KR250_002316"
     CDS             12062..13924
                     /locus_tag="KR250_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0068247.1"
                     /translation="MSEKRIDEQDIEVLSFEDGMSAEKAKDAKKTTKRLLKYMAKQKF
                     KILIIFISVLISSALTVLAPMVMGKAIDQLFNGIKTAVQTGTKFSVNFSTMGGIVSIL
                     LGLYLISAVFIYIQQYIMSGVAQNLVLSMRKDLSDKLNKLPLKYYDSHKKGETLSIVT
                     NDLEKVADSLQEGLMQLITAVVTVVGSIVMMISISVPLTIVSAITLLVSLGITVVIAR
                     KSQERFSENQKALGELNSNIEEIFTGQIVVKAFSKEKDTIRNFKILNQNLYNASRKAQ
                     FSSYAISPIIRFINLIGYVIIAVVGGIFASTGAMTLGSIQAFIQYVNQASEPTTEISY
                     IVNMLQAAIASAERVFTVMDEVEEIKDEESSKVISMPKGKVQFEHVKFGYSDDFILMK
                     DININLNAGDKIAIVGPTGAGKTTLVNLLMRFYEIQGGRITIDGVNIKDLKRGELRTM
                     FGMVLQDTWLFNGSIKENIAYSKSDATMDEIVRAAKSARVDHFIRTLPQGYDTILTED
                     ASNLSQGQKQLLTIARAILSDPSVLILDEATSSVDTRTEVEIQKAMNNLMKGRTSFVI
                     AHRLSTIRDADLILVMKEGTIIEQGSHKELIEKKGFYEELYNSQFTSEYDEDVV"
     gene            14257..14946
                     /locus_tag="KR250_002317"
     CDS             14257..14946
                     /locus_tag="KR250_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HBH0068248.1"
                     /translation="MNKKLNYRFEMKHKITEADVLALKSRLYQIMKKDENASKDGKYL
                     IRSLYFDTPEDKALLDKLNGVAIREKFRIRFYNNDCSYIRLEKKIKHYNMTSKLSTNL
                     TKKEVINILNNDIEFLKESTNCLLREFYLKLKCERLEAKSIVDYNREAFIYPVGNVRV
                     TIDSDIKTSVNSVDLFNKDLPTVSVIDENMTVLEVKYDEFIPDFIKDLIQINKTTSTA
                     VSKYASSRLYI"
     gene            15051..15737
                     /locus_tag="KR250_002318"
     CDS             15051..15737
                     /locus_tag="KR250_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HBH0068249.1"
                     /translation="MILIMTFNDIFKSSFIENVSGFSFVDSALALGSAFLVGLFIYMV
                     YKKTYMGIMYSRPFNVSLVALTMLTTFVILAVTSNVVLSLGMVGALSIVRFRTAIKDP
                     MDLVFLFWSLGSGIVLGAGLIPLAVMGSIIMGLILIFFSNKTISETPYILMVNCNNED
                     SEDIATDKIKKVFTKYQIKSKSVTPEKGIELVFEVRMKDGETSLINDLSQVDGVTNAV
                     LVSYNGDYVA"
     gene            15754..17661
                     /locus_tag="KR250_002319"
     CDS             15754..17661
                     /locus_tag="KR250_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HBH0068250.1"
                     /translation="MKDKKFTLLISIMIIFLCAVVGVYSTSSNKSVDLYSDVYIEKYF
                     NRDKVMEVNIEIDESDLKDMNENAIKEEFKVAKVTVDGDTYGNVGIRTKGNSSLISVA
                     NSDSDRYSYKINFDKYNTSQSMEGLTQLNLNNCYSDPSYMREFLTYSICEEMGLATPE
                     FAYAKVSINGEYHGLYLAVEGLKESYLENNFGNVTGDLYKSDEGSSLQYKGDDPESYS
                     NLIVESDKKTADWSKITKLLKSLDTGEDIEKYLDVDSVLKNIAINTALLNLDSYQGSF
                     AHNYYLYEQDGVFSMLPWDFNMSFGGFSGFGGGSQSIAIDEPTTGNLEDRPLISSLLK
                     NETYKTKYHKYLEEIVTKYLDSDYLENMTTKLHDMIASYVKEDPTAFYTYEEFEKNIT
                     SSIEDSSDNKGFGNKGFDNNNSNNSDSNNNSNSENKRSGNQSDEKEVNAELTSSVVKA
                     NTDNETKNKTTNDSESKNNTDKDKSGNDNNQKLEGPMGKGGKSIPGVLEVAEDMSKTI
                     KSQLSGETSSTKQNSGDESSSGIKGSEKFDEDMSGMPEPPEGMDGKMPPGMGNMDKGD
                     MNGKNGNMNMDRNQDNPIEVGGFGNRGGGSVSKTTTYFKLILGGASMIIMSIMLVGVS
                     RVKRRRFIKSK"
     gene            18112..18801
                     /locus_tag="KR250_002320"
     CDS             18112..18801
                     /locus_tag="KR250_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0068251.1"
                     /translation="MLQKVLWKFLKLVVGLFICSVGIVLTINCNLGLSPWDVFHQGLS
                     NHIGITIGTASIIVGSIVVIADVVLGENVGWGTVFNMLLIGFFMDLLLYSNLIPEADN
                     LFVGIIMLILGLVLLSVGMVFYMGSGLGSGPRDGLMVAIQKKTGKSLKLIRGTIEVGA
                     LIVGFLLGGKVGIGTIISAFGLGYFTQMVFGLFKLDCSKIKHRFIVDDIKFIKVYMSG
                     EKKAAVQNIVK"
     gene            19237..19776
                     /locus_tag="KR250_002321"
     CDS             19237..19776
                     /locus_tag="KR250_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007286148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0068252.1"
                     /translation="MDIGEKIKRLRTEKQLTQEELANRCELSKGFISQLENNLTSPSI
                     ATLIDILEILGTNLREFFNEIDDERISFTKEDMFETEDEDLKYKLKWLIPNSQKNEME
                     PIIITLYPGGQYKEEKPHEGEEFGYVLAGSIYVHIGEKKNKVKKGESFYFRPKANHYI
                     SNEGKTTAKVIWVSTPPSF"
     gene            19795..20838
                     /locus_tag="KR250_002322"
     CDS             19795..20838
                     /locus_tag="KR250_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0068253.1"
                     /translation="MVENIIELRDISKHYEELTVLDNFNLDIKKNEFLTLLGPSGCGK
                     TTTLKIIAGFEYADDGKVLFEGKEINNLPPYERQVNTVFQKYALFPHMDVYENVAFGL
                     KIKKTPKDVIDAKVKEVLKLVALEGFERRQVESLSGGQQQRIAIARALVNEPKVLLLD
                     EPLGALDLKLRQEMQIELKRIQKKLGITFVFVTHDQEEALTMSDTIVVMYKGKIQQMG
                     TPQDIYNEPKNSFVAKFIGESNIFDGIMIEDYKVNFCNRDFECVDKGFEKNENIEVVI
                     RPEDIKMVKPEEGMLKGKVTSTVFKGVHYEIELNENGRLWILHNTKNAEVGTELGMDI
                     YPEDIHIMRKENN"
     gene            20840..21676
                     /locus_tag="KR250_002323"
     CDS             20840..21676
                     /locus_tag="KR250_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0068254.1"
                     /translation="MKRKSFLAYPYVVWSAIFVIIPLILVVFFSFTKESGGGYAFTLE
                     NYKEVIDPIYMKVFGRSILLAGGATLICLIVGYPVAYIISKARVSRRGSLILLFILPM
                     WMNFLLRTYAWVAILGKNGLLNTFLGWFGIQPLAILYTNFAILLGMVYNFLPFMVLPI
                     YTALSKMDNDLINAAHDLGANNMTVFRKIIFPLSLPGVMSGITMVFMPAVTTFAISRL
                     LGGGKIMLVGDLIEQQFTVVGDWNFGSAISIFMMIVILISMSIMSKFGDESDKEGGGL
                     LF"
     gene            21781..22455
                     /locus_tag="KR250_002324"
     CDS             21781..22455
                     /locus_tag="KR250_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0068255.1"
                     /translation="MARWNGFTWKWYGQLLQNESIMSALYYTIVIAILASVISTIVGT
                     ISAIGIHKMRGKSKKLILNVNYLPILNTEIVTAVALMSLFVFVKMEFGFTTMLLAHIM
                     FCLPYVILSVLPKMKQLPDNIEDAAMDLGATPIYALRKVILPQIKPGIVSGFLIAFTM
                     SIDDFIISFFNAGNGVSNLSIEIYGMARRGIKPEINALSTIMFAVVLGLLLLANKKES
                     IVRGIK"
     gene            22455..23507
                     /locus_tag="KR250_002325"
     CDS             22455..23507
                     /locus_tag="KR250_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HBH0068256.1"
                     /translation="MIKFKKAISLIAVGMMTVTLFTGCNKATDSTKVLNVYNVGDYID
                     ESLIDKFEKETGIDVQYSTYDTNEMMYQKVKSGSTHYDLVFPSDYMVEKMKNEGLLEK
                     LDFKNIPNMKYLDKSFLNPVYDKTNEYSVAYMWGTLGILYNKKEVKDPMDSWNILWNP
                     KYKGNIMMFDSVRDTIGITLKKLGYSMNSVNPKEINEAKNLLMKQKDLVLAYVNDEGK
                     DRLLGGEVAMGMLYSGDAVTLMEQNPDLDYAIPKEGTNKWVDAMCVPTTAQNKKEAEL
                     FINFLLDPENAKVNAEYIGYSTPNTGALKLLDPEITQNPVAYPSKEVLDKCETFEDIG
                     ENIKLYDKAWIELKSK"
     gene            23737..24585
                     /locus_tag="KR250_002326"
     CDS             23737..24585
                     /locus_tag="KR250_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBH0068257.1"
                     /translation="MNRINKKIDLLILALLVFIFLIVGIVILSIRTENKLNVFIMLAI
                     IFFIIMLTYLSNSVVGLITSSIIIFMYTSYILYNNITHNMDVEFISYMWIIATPVSSI
                     IMGNLNKSINELQNTNKKLSEQYKELVTIDSETGLRNLKIFYNDVNMEISKSIRHNTD
                     FSLMIVKLPYYGNLQTIFGENKTNKIVKYIGSNIIECTRNEDIIYSLQKDMIGILMPN
                     TSLEGSKVVKDRIKKRIKELNLDLNNRGKYVNIDVKIAFLQYKSSFGDSINFKNIVEE
                     ELQYDV"
     gene            24575..26239
                     /locus_tag="KR250_002327"
     CDS             24575..26239
                     /locus_tag="KR250_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBH0068258.1"
                     /translation="MMFKIFNKSLLKLVVVLSIVLTISNFSLSSYASEITSSTDKVLI
                     IYDSKKETAYNRDILNIMRTLLGRFSSDIELLKLSNYDGEINKNYYSHIFILGINENS
                     YNNDKNTKNLISSLNSYKGTICWLGYGIENLLEHKKYNLDYVGKTNNIVSVNYRGKSY
                     NLDEHYVFNIVESKDTSNKVIGSINDTLNKYPYIINDKNLFYVSKLDLDGVLFYIFCD
                     SLNDIFNIKTFDKGRIFVRIEDVHAFREPKNLVEIADYLSSKNIPFTIALIPAYVNPK
                     NHKVITLSESPEIVKAIKYMQDKGGTVILHGYTHQYKKEEVSGEGYEFWDGKKDEPLK
                     ENMKIFVKDRVLNGLRVCIENGIYPLAFEAPHYAMESEGYKELKKYFSTYMGQHQNND
                     KKFSTNTYPYIIRDTEEFNIFIPENLGYIDPEDKFTFQNIKENLNKLSIVRGFSGGFF
                     FHSYLNIEYLKNTIEYLEKQNIEFMNLRDFNNWVKVDEIQIRNNGDEIIVNYDKDLDE
                     ITKSDTRFKSISNISKVLIFIVSISVLIFVIIFIYFKRIDKKKFLK"
     gene            26288..27544
                     /locus_tag="KR250_002328"
     CDS             26288..27544
                     /locus_tag="KR250_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003362983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBH0068259.1"
                     /translation="MRDLNVSEYLFVFSLFSIWSLLLINIILAMGGYIFYFKNFDKEI
                     KEIDEYPMISILVPAHNEAKVIGRTVESLLLLNYPKSKMELIVINDNSSDNSKEILEN
                     IKDRYNNYNFTIINTDSLTGGKGKSNALNIGYTISKGDFIAVYDADNTPDKNALRYLV
                     QTIVMNDELGAVIGKFRTRNKNKNLLTKFINIETLSFQWMSQAGRWQLFNLCTIPGTN
                     FILRRSIIEEIGGWDSKAIAEDTEISFRIYKLGYKIKLVPQSITWEQEPETVKVWIKQ
                     RTRWAKGNIYVLMKYIKNIFKQGRNKIVFDIAYFFSVYFLFLTSVIISDILFVLSISK
                     LVEISIPINFFLIWILSYLLFIIEVSISLTIEKGEATIENIFIVAIMYFTYSQLWLFV
                     AIKGMIEYLKDIIFKREVKWYKTERF"
     gene            27567..29648
                     /locus_tag="KR250_002329"
     CDS             27567..29648
                     /locus_tag="KR250_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HBH0068260.1"
                     /translation="MKKFIISIISLVLFFSNISLIYKVNADETKVKNYKFERDITIDG
                     VIGSNSTFFEVNKNWDIEEVLLHLNFSKSQILNGDVSSLTVLINNVPIKSIKLNAKTN
                     YKNTLEVLVPKDYIIQGYNEIKIKTYKTISDKICQDDSNTGNWMVIHKESYISIRYKQ
                     KKVENSINEYPYPYAEIENNHKLDTTIVVPDNMTRGETTAVFNLASAFGKITKNDDLK
                     LDVKLYSEMKNWSDDNIIYIGKPENTAEEILDILSIKEQTLLSSNCIIKQVDSPYNKN
                     KKMMVVIGSNEDDLIKASNLLIENRLSNQVLSSSVLVNKETNIKINREQKLNLGHLTL
                     KDLGYSDFLLEGAFNQQALFDVKIPTGKVLDDGSKIILNLRYSDNLDFEKSLVTVSIN
                     DVIVGSKKLDRSHSNNDKLELKIPKDIDNKNYYQVKLTFNLSIKNSNCVTRESNNPWA
                     YVSNNSYLALSMKENETLSFENYPYPFVRDDEFNDLTVIMPDYSGSQAMTWMFRLGVT
                     LGANINSHNGNINVIRGKEFSDKYKDTNIVVFGVPHNNSVIKMLNNNLNIKFDKNYSN
                     FISNDKISFIDDYGKNISTIQLIKSPYNNQKNIMVISSMNEKNLYLGMDYLLNKSKVN
                     DLKGDTLIIDEYGEVEDLAYNLKSKKEVKDSSWNMSINKTTKVFLMISFITIIVVMIL
                     SMLYIKKYKRR"
     gene            29685..30770
                     /locus_tag="KR250_002330"
     CDS             29685..30770
                     /locus_tag="KR250_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBH0068261.1"
                     /translation="MRKYMNKFKLKRSILISLIILIFIFIVYFVSIELKDRSLVNNQY
                     KGKIKSANLSVDYEINQVMKDIDKLGLNTVNVPIIINVESINSDIMSIDNNSKEKAIK
                     LIKKLNKKGISTILEAYPWIENGKLYETDWNPINKKRFFYTWQNAILNELIIDVANPL
                     DVNVLNIGSNFVHLEEYQQNWGEIIDFVQSKFDGFVTYRTNWWYTKKDDLSSKLFYEQ
                     KLNNNFFDKLDFISIAAYFELSNKPVNTVDELISALHSSTVNNRGQNIKQEIYNLYKA
                     HRKPIFFGELGFSNRESASSQPWNHTPSKAVNGEEQARCFEAYKKVFENEDWINGFSV
                     FCVGKIDDEKNFYPSKESIKVIKSWYE"
     gene            30824..31948
                     /gene="wecB"
                     /locus_tag="KR250_002331"
     CDS             30824..31948
                     /gene="wecB"
                     /locus_tag="KR250_002331"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HBH0068262.1"
                     /translation="MNDIKVMTVFGTRPEAIKVAPLIKELEKRENIKSIVCVTAQHRE
                     MLDQVIETFNINVDYDLDIMEKGQSLNDITCKILNKLPLILNKENPNIILVHGDTTTT
                     LATSLTAFYNKTLVGHIEAGLRTYDKYSPFPEELNRQLTGIIADMHFAPTNLARKNLI
                     SEGKPNNNIFVTGNTAIDALKMTIKENYNHPIIDEIGNDRMILLTSHRRENLGKPMKN
                     IFRAIKRIVDDFEDVQIVYPIHLNPKIRTIADEIFGKFPEKIHIIEPLDVADFHNFLN
                     KSYMIMTDSGGMQEEAPSLGKPVLVLRDKTERTEGIEAKTLKLVGTNEDRIYNSVSDL
                     LINKDNYVQMSKASNPYGDGNASKYIVDIIIKKFNCKYLN"
     gene            32026..33000
                     /locus_tag="KR250_002332"
     CDS             32026..33000
                     /locus_tag="KR250_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HBH0068263.1"
                     /translation="MARKLIKNLGKSKSVKRVKLLFKKIFITVFIVASIVAIFNITKY
                     FEELYKVRDLKSTKIEYYMDVADEAGDGKVQLSWKALLAIDMVIHDEDLSNIKKKDTL
                     DIGEKFIVEDKNDKGEKVYKVKKFNKVLSELKFDSSQKSRARKYMKDLEYTYLGNKQL
                     DSSDEKIKFIKKLEDSAIREYIDYGILPSITIGQAILESGWGNSKLTKQSNNLFGIKA
                     DKAWKGKSVEISTSEHYNEKIVASFRSYNSLQDSVKDHSLFLINNKRYRKHGLFEAKD
                     YISQAQSLENAGYSTAEDKKGNRIYAELLIDVIRSYNLQLIDNKVETK"
BASE COUNT        13199 a         3361 c         5598 g        10985 t
ORIGIN      
        1 atttctataa agatgatgtg aatctaaaca ttatatgaca acgtgagcat ttaagttaaa
       61 atattttaaa tatactattt tagataagtt ccctcttcat cttgataaat taatcctttt
      121 ttcataagca ctccaagagc tcgtttaaaa tttttcttac ttgaattaaa taatatagaa
      181 atatctttag ggtcagattt atcattaaat ctcatatatc catcagaacc ttctaaataa
      241 cttaaaatct tattttctaa agtatcaagt tcatcctttc tatttcctct aggagaaaga
      301 ccaagttttc catcttcata tatttttatg acatgtaaat tttcaagaac atcaccagtt
      361 tttagttcag taaagtactc attatgaagt attacaccag catatttatt gtctacacat
      421 atcattgcag aattattagt ttggaatcca tatacaactc cagttacaat atctcctaca
      481 ttgtaagtat ggtctgttaa taagtatgaa tctatatctg tagtagcagc aattcttttg
      541 cttttgtcta aatatatata aaataaatat ttttcgtctc taaatagtgg ataagtctta
      601 gctttaaaag gaactaatat gtctttagga agtcccatat ctataaaagt accaatgtct
      661 gtattatcaa ctacttttaa atgtgctaca tccccaacct tagctaaagg cggtattaaa
      721 gttgcagcag ttctgtcatc agaatcttta aatatgaaag catttacttc atcacctatg
      781 tttatttcat tttttccgat taatctatta tgaagaagta tatcgtcttt tgtgttatta
      841 gtttgtccat ccaaaaaata tccaaattca gtttttcttt ttactgtaag tttattaaaa
      901 tcacctatgt ttatcaaatt aaaatcatcc tctcatttta tatttataat ttagccataa
      961 tatacttatg tagtagtttg tcacagtatc tatattagta ttagtgaaat ttacaataaa
     1021 taatatagtt atttatgtat ttttcaattc tctagcttct tatatagtat aaactccaat
     1081 ttcttttttg tctttaggtt ttattaaaat ttttacaaaa taagttaaaa agtattgaaa
     1141 taaaatttct tttaaagtat aattggtata taccaaaatt ggtatatacc atatcgaaaa
     1201 gtggtgacta tatgaaaata gtaaataaat ctagtaatat accattgcat actcaattaa
     1261 gttcaataat aagagagatg atagaaactg gtgagcttaa agagggcgat gcaataatgc
     1321 cagaaagaga actttgcaac atacaaaatg taagtcgtat gacagtaaat aaaaccattg
     1381 ttggattagt tacagaagga cttctttata gggtacaagg taaagggaca ttcgttgcaa
     1441 agcaaaaaaa gaagtatcag ttttcaaatg taaaaggttt tactgatgtt atgaaagaaa
     1501 agggagtaaa cataaaaacg gatatattat cctttgagat ggaactacca gatgatttag
     1561 ttaaaagaaa gcttggaata agtgataata ctacaaatat atataagatt gtcagactta
     1621 gatatactga tggagagcca tttggattag aaatagtata tctatcagaa gaaatgtgta
     1681 agggtctaac aaaagggata ttggataatt cttcattata tagggtttta aatgaaaaat
     1741 atggctataa gatacaaaaa gctgaacagg taatggagcc agtaatactt tcagatgaag
     1801 aaagtaagct tcttgaaaca gatgaaggtg ctttagctct taaacttcat agaaattcct
     1861 ataatagaga aggtagacct atagaatata ccatatcaat atttagaaca gataaatatc
     1921 aatatgaaat agtattgagt gagtaggtga ataatatgaa atgtttaatt aatggaaaga
     1981 taatattaaa aaatcaaatc ttagaaaata aagtattagt atttgatgaa aaaataattg
     2041 atatagctga tagtgtacca aaagattgtg aagttataga tgctgatgga aaatatatat
     2101 ctccagggct tattgatatt catatacatg gaaacatggg aaaagataca atggattcga
     2161 ctgatgaatc tattgaaaca atttcaaaat caattatgag acatggagta acatcttttc
     2221 ttccaactac aatgactatg gataaagaac atgtttatga tgcacttgaa gttattaaaa
     2281 aagcccaaaa tagaaagctt gaaggggcac aagtattagg agcacattta gaaggacctt
     2341 ttataaatga gaattataag ggagcacaaa atgaaaaatt tataataaat tcaaaatacg
     2401 aatttataaa agaatataaa gatgttataa aagttataac ttatgctcca gaaaaagata
     2461 ttgattttga ttttactaga gaaataaaaa gatgtacaga tatagtttta tctattggtc
     2521 atagtaatgc aaattatgac caagctaaag aagctataaa tttaggagta actaatgtaa
     2581 ctcacatgtt caatgcaatg actggtttaa accatagaga cccaggcgtt gttggtgcag
     2641 cacttacaac taatgtgtac agtgagttaa tagcagatac aatccatata aataaagatt
     2701 tatttcaatt tatactaaat aataaaggaa aagaaagact gattttaata acagattcta
     2761 tagaagctgg tggtcttgaa gatggcaatt attctcttgg aggtcagaaa gtcatagtta
     2821 agggtaatga agctagactt gaaaatggag cattagctgg aagtgtgttg tctttaaata
     2881 aaatggtatt taacttctta aataatacaa accttaaagt gaatgaagca attaatttag
     2941 cttctttaaa tccagcaaca tctttgggaa taaatgacaa aaaaggaagc ttagaaatag
     3001 gtaaggatgc agatatagct gtatttgatg agaatttaga ttgtaaaatg acactttgct
     3061 tgggggaagt agtatataaa aatatatagt atacaaaaca aaccttaaag acagtaaaaa
     3121 caatataaaa aataataaat taaacttaca ttagtaaaag tagtagttaa aatttaggag
     3181 gaataagatg agagtattag tgtgtaaaga ttatgatgga atgagtaaaa aagccgctga
     3241 aatgatagca gctcaaatag ttttaaaacc aaattcaata ctgggactag ctacaggaag
     3301 tacaccagta ggtatgtata gagatttagt aaaaaaatac aatgataata tagtagattt
     3361 ttcagatgtg atgagcttta atttagatga atattataag ttaccaatat ctaatgacca
     3421 aagctatgat tattttatga aagagaatct atttaatcat gtaaacataa agccagaaaa
     3481 tactcatcta ccaaatggaa tggctgatga tattgaaaaa gagtgtatga actatgaagc
     3541 ttctatagat gcagctggtg gtatagatgt acaagtactt ggaataggtc gtaatgctca
     3601 tataggtttt aatgaacctg atactaaatt tgcaaaaaga actcatgtag ttgaacttac
     3661 agaatctaca atagaagcta atgcaagatt ctttaaatca agagaagatg ttcctaaaaa
     3721 agctgtaagt atgggaatag gttcaatatt gaagtctaag aaaatattgc ttttagcttc
     3781 aggagaagaa aaagctgatg cagtatataa tactgtttac ggagatataa ctccagaagt
     3841 tcctggttct atacttcaac ttcataaaga cactatagtt atagtagatg aagcagcagc
     3901 aagtaaattg aatccaaaag attataaatt agtataagaa tagaatgtag tttaaataaa
     3961 aaatagctat cattgaataa tcaaatttat ttaatgatag ctatttttat ttaaattagt
     4021 tattattgtt taaattactt gcttattact ttttacgaaa actcgttatc aagaagtatc
     4081 caaataagat gttaaaaatt attatgttta gtgggattct atcaagcata ataaaattac
     4141 ctataattgg gatattacca actggaaaca taaagaagaa tacaggcatc agtgatagta
     4201 aaaaagatgg aaaacaaaga agaagtaatc ttggaatatc aatataccaa ctaccatcct
     4261 ttttgtattc tctaatcaat aattcagttc ctagtaataa accaatacca ccagaggaaa
     4321 ttaaattaat aaaaatgtat cttttaaagt cttgcatata accagtgtaa aattgagtat
     4381 agaaatcata atttaattta acggctaaaa aatacataag caatagaatg attaaaaata
     4441 aaagatatct aagagctttt ttcattttaa attctccctt cagaaatact ttatttagtt
     4501 ttattttgtt ggaatctgat tttatcattt agtttaagaa atccataata ttagtgaaga
     4561 atgaaataag tattatatat gaataaatat ttttagaatt aaggtgaatt gtagtgctta
     4621 atataactat aatattaatt gtttgaatat tcaataaaaa gttcgaaaaa tatcaattct
     4681 gatataaact gaataaatac agttgaatat taaattatta ttttatcaaa aatatgcaaa
     4741 gtactacacc ttcaaatatc acctaaagta attaaaaggc aattaaagtg aagtagttca
     4801 agtatttcta aaataagttt aataaattct ataataattt attttttgcc ttaaaattgc
     4861 cttaaaatcc aaacaatttt aaaactaatg tactataaaa agctggtata atttaaatat
     4921 agtaaaaaat aagatttatc ttatctattg ggatttaatt atttaatcaa tatatttata
     4981 ccatttaatt taaataaaaa gtctattaag gagtgagctt atgagtaagt taatttatat
     5041 agcagatgat gaagataata taagaaattt ggtaaagaca ttcttaaaaa atgaaggtca
     5101 tgatgttatg gattttaaaa cgggtgatga acttttagaa caatttaata taaaggagtg
     5161 tgacttagta atactagata taatgatgcc tggctcaagt ggttttgaag tatgtacaaa
     5221 acttcgtgag aaaagtacag tcccaataat catgcttact gcaagagata cagatataga
     5281 ttatattaca gggataacct taggtagtga tgattatttt acaaaaccat ttagcccaat
     5341 gtctttggtt atgagagtaa aatctatatt tagaaggata gagtttgaaa agaaacaaaa
     5401 ttatgataaa tatagtaact cgatagatat ggaattaaaa tttggtgatg taataataaa
     5461 taaaaagaat aaaatcgtta caagtaaaaa tgtaaatata gaccttacac caaatgaata
     5521 taacctattg acttatttgt ttgaaaatat agatagagca atttccagag atgaattatt
     5581 aaacaaaata tggggatacg atatagaagt tgagacaagg gctgctgatg atacagtgaa
     5641 gagattacgt aaaaaaattt tagatacaaa tattttaata gaaactgtat gggggtttgg
     5701 atttaggttg aaggagaaaa gttaagatga agaatggaaa tgacaagaat agaattggaa
     5761 agattaaatc caaatttaaa ttaaagttct caaagaaaaa tataagcata aaaactagga
     5821 ttttgttgag tatactttgt ttgataatta tagtatttat gataatatta gtatctttta
     5881 atgtatttct tgatacatat ataaaaacaa ctgcgaatga agaattgaca aagtctacag
     5941 aaatagtaga acatatggat agtaatttta gacctattaa gccaccacaa gatgatggaa
     6001 agcttcctcc caaagtatta tctaatttta tgagaaatgt acaagataaa gttaggatgg
     6061 cagaaacaca atcagatgct gatgctatgg tagttgattc taagtataac ctgatatttc
     6121 caacaagaga ggacgatttc ttaaaaaatg ttgatgagat ggagctgatt agggagtgta
     6181 ttcaaaatga aaaattagat ttaaattcag atgcaaatac acggatatct accagaaata
     6241 aggattatta tatttcaact gtaaaaatta ccagtatata tggtgaacaa gacaagtatc
     6301 ttatactttt tatagacata tcaaaaactc taaatctagc acaaaagata aatattgtat
     6361 taatatctgt aatgtgtttt gctggaatat tagcaatatt tacagctgtg attttatctg
     6421 agaaaattgc taaaccgata aaagagttgt gtgagtttgc aaaaaaaatg ggtcaaggag
     6481 atttcaaaag aacctacttt gacttttcag ataaagaatt agtagagctc tcaactgtta
     6541 tgaataagag tgctgagtat ctggataagt atgataatga acaaaaaata ttttttcaaa
     6601 atgcatctca tgaattaagg acaccactta tgtctattaa aggttatgca gaagctataa
     6661 aatataatgt tatagatagt aaacatgcaa gtgatataat tcttgaagaa agtgataggt
     6721 taagtgatat ggttgaagat ttattataca tttctaaaat agataatata actaaagatt
     6781 ttgaacttgt agaatgtgat ttaagagaag tactttcaaa ctgtggagct aggcaaaatg
     6841 taagagctat aaataagggg ataaagttta tttatgaatt tgatgaagaa atggttttat
     6901 ttgaatgtga tgaaaaaaac atttccaaag catttatgaa tcttatagaa aatgctttga
     6961 gatacgctaa gagtgaaata aaaatagtgt gtaagtataa tcaaaaaaat atagttgtga
     7021 taatagaaga tgatggaatt ggaatcaaga aagaagattt acctcatgtt tttgaaagat
     7081 tctataaggg agtaggagga aatcatggaa ttggattatc aatagttaaa tctattgtaa
     7141 ataagcatgg tgggcgtata tacgtagaaa atggcaaaaa aggagctaaa tttactatat
     7201 tatttaaatt gtaaaaaaat tagagaaaat atagagatgt ttgaagttgt tttttatata
     7261 tatctgttgt atactgttga aaaaagaaca atattaagct gacatatatt acgatagttg
     7321 acatttaaag taaatgctta aatatagata cgaaaggaaa agtaaaatct gaagtgtatt
     7381 taggtttaag taagcttctt attttataaa aaatatgtat gaaagaacag ctgattttta
     7441 atattacaaa tttagtaacg gttgtatttg aagcttttgt catccatatg tttttatcag
     7501 actttttagg ccagaaagaa gggtatataa aagttgtaag atatgctaaa ctgggtttta
     7561 taatatgttt aggattctgt aatttaataa ctttaaaccc taaaatcaca atgcctttaa
     7621 ttttcatcct tattttttca acatcatttt tatataaagg aaatttaaag acacgattgt
     7681 ttactactgt tttattaagt atattcttta tactgtcaga aattgtagtt acaagtattt
     7741 ttgttttatt tgtaaaagaa ggctttgaaa taatgcttga aaataatagt ataagagtat
     7801 tggcgacaat tttatctaaa attgtctttt tgttgacttg taaaattatt tgtctattta
     7861 aaaaagatgt acatttagat atgcccataa agtattggct ccctttattt ttaataccta
     7921 tatttagttt atttctgtct gtttctatct ttgatgtaag taaatttttt agtcttgaaa
     7981 gtttaaaatt tctaagctta atatctagtg ttggtatttt atatattaat tttattgtct
     8041 tttatttatt taaatttatt atagataaaa ctaaactatc tatgaaatat gaactacttg
     8101 aaaaagaaat aattcataag gaagaattaa gacttagtaa tgagtgttat aaaattattg
     8161 ttgaacagac tgattctgtc gttttcgaat ggaatatcaa agaaaataaa tcatttgtat
     8221 cgcaggcttg gactgaaaaa tttggatata acaatgcatg taagaatatt tttaaagaaa
     8281 ttaaagataa agacttagtt cattctgaag ataaagctat atttgaaggt tttcttgaaa
     8341 gtattaaaaa aaagaatatg cacaatcaag cagtatatcg tttaaaaaaa tctaatggtg
     8401 aatatatttg gtgtagaaca tcaattacaa gcatttataa tgatgaaaat gaacttttaa
     8461 gagttgtcgg agtaattgta gatgtagata gtgatataaa aaaatatgaa gagttaagaa
     8521 ctagagcaga aagtgactca ttgaccaata tatacaataa aggtacattt gaaaaattag
     8581 tggaagaaac tatagttatg aatacaggtg ataaaaaaga tgcattattc attattgatt
     8641 tggatgattt taaagaaata aatgataatt ttggtcatcc atttggtgat tttgtactaa
     8701 aaacatttgc tgataagata caaactagtt ttggtagtaa agacttagtc ggaagaattg
     8761 gtggagatga atttgttgta tatatgcaag attatgttac agaagtaaat ttgcataaaa
     8821 aagctaaaga attaaataga gtactttcgg ataattacac tgatttgagt tttagttttg
     8881 atgcttctgt cagtataggt attgcaaggt atcctcaaga tggaacttca ttttttgaat
     8941 tgtttaaaaa tgcagataga gcactttata gcataaaagc atcaggtaaa aattcatatt
     9001 gtttatttga agaagaacta tatgtacagt gaatttgaat ttagatttta tgtgagagta
     9061 agatttatat atacttgtgg ctgtctgatt tataaaatat gttactttgt atatttttat
     9121 atgtttcagt aaggttaatt aacataagca aaggtgtaag taaatttata ttagtttaag
     9181 tttacttaca cctttataaa aatacaaata atagattcat ttacattctt aaatgattaa
     9241 agctctttac cttctaaaca tgcataacat atcgttataa ttccagtaaa taagactaaa
     9301 atacccattc ctaagattac agtttcatta tctattactc caaatgtttt taaagtaact
     9361 tatttcgctt tgtatcataa taatttgtct ctttttacat tttaacttct gctgagattt
     9421 gagttagata ttcgttagaa tctcgcacac ttatatcata tagaagatat tcttcataaa
     9481 caaaatcccc aatacgtaaa ttattctgat taaaaaaatt taataacttt tcataagcct
     9541 tgtaaatagt ttcataactc cctttatgat aagcagttac ataaagtccc tttggtttga
     9601 ttgatacagg atatttcttg tactctttat taacttttgt aaataaatat gaatatgatt
     9661 caaagtcttt attaattata tcttcacctt taatgattga gccaatcgta ccataatcac
     9721 tcaattcata gttgtccata aagttaatac attcatataa aaaattttct tcctcatctc
     9781 ctaaaaactg ttctttatat gtaacaggtg ttttgaccaa gtactcttct tcatgttctt
     9841 ttaaagtaat agtattcgca tctatattac aggcattttt agtgaatgaa atagtagatt
     9901 ccattagagc ttgaatatta tttagttttt caatttcatt tttaatatca atggttttct
     9961 tttctaaaag ctcaattagt aaattaggtg ttcttttgtc tatataagct ttaatttctt
    10021 ttaaaggcat ttttacttcc ctaagtgaag aaattatacc aaaaatgcta agttgatggt
    10081 atgagtagta tctatatcca ttttctttct ttaattctgg tgagaaaaga cctatatcat
    10141 cataatggaa aagtgttttt ttattgattc cacaaatctt tgcaaattct ccagttgtaa
    10201 aataattttt tgaaacttga ctcataatta taattcctcc ttgactatcc tgttacagta
    10261 tagtttatca tagtattttg taaaggagtg tgattatatg aaaaaattaa ttcatttttt
    10321 aaaaccatat agagttttaa ttgttgtagt tttgatattt acgtttttac aaacacttgg
    10381 aactttgtat ataccaacac ttacagcaaa tatagtaaac aatggtgtag taaagggaga
    10441 tatagattat attgtaaaaa caggtttaat gatgatgata gtagctggta taacagcttt
    10501 atcagctgtg ttagtttgta aagtatcagc aaatctttca tctggatttt gtagagatat
    10561 tagagaagca gtatttataa aatcacaaga tttatcaata aatgatttta ataatatagg
    10621 tacagcatca atgattacta ggagtactag tgatattact ttaataggtc agtctgtgtt
    10681 tatgtttatt caattagtat taccagcacc aattataact gtttctggtc tattcttagc
    10741 atattctata gataaagcaa tgacaattat tattgttgta gtaatgtttt tattcatgct
    10801 ttcagcattt ttagttggta aaaagcttat aaagttgttt aaaatgatgc aaataaaaat
    10861 ggacaatatg aatagggtgt tacgtgaggt agtaactgga gtaagagtta taagagcttt
    10921 taataggagt cattttgaaa aaaagagatt tgatagaact gcaatagatt attctgaaac
    10981 agcaatttca ataaataaaa tatttgcagt acttatgcct attgtaatgt taataatgaa
    11041 tttaggtata gtaagtatta tttggtttgg tgggatgaga gtcagtaatg gaaatatgga
    11101 aataggtcat ataatggcac ttgttgaata ttgtatctta attttattct atttaatcat
    11161 gggagtaatg gtatttatgt acatacctag agcaggtgca tgtgcagata gagtaaatca
    11221 gatattagat atagaacctg aaattgtaga tggaaatggt cataaagata ctgtctcaga
    11281 gagaggacat ttagtattta aaaatgtaac cttttcatat gcacaatcag aagaacctgt
    11341 gttaaataat ataacatttg aagcaaaatc tggagaagta actgcaatta taggaagcac
    11401 tggctcaggc aaatctacta ttgcaaatat aataccacgt ttttttgaaa ttcaatctgg
    11461 agaaatatct ataaatggtc aggatattaa gaagattcct caaaaagaac ttagagataa
    11521 gattggattt gtaccacaaa aagctttttt atttagtgga acaatagaag aaaatataag
    11581 gtatggaaaa gaagatgcaa gtattgaaga agtaaagcat gctgcaagta tagctcaagc
    11641 tgatgaattt atttctgata tggaagacaa gtatgactca tttgtagcac aaggtggaaa
    11701 taatttgtca ggtggtcaaa aacagagaat atccattgca agagcgttag ttagaaagcc
    11761 tgaagtatat gtctttgatg atagtttttc tgcattagat tttaaaacag ataaaagact
    11821 tagaaaagcg ttgaaaaatg aaatcaaaga ttcatcagct ataattatag cacaacgtat
    11881 aagtacaatc atggatgcta atcagattat agttttaaat gatggaaaaa ttgtaggtat
    11941 tggaaaacat aaagatttac ttgaaaattg tgaagtttat aagcaaattg cggattcaca
    12001 acttagtaaa gaagaacttg cataaaatga agaattaatc tgatatttgg aggtgtatga
    12061 tatgagtgaa aaaagaatag atgaacaaga tatagaagta cttagttttg aagatggcat
    12121 gtcagcagaa aaagctaagg atgcaaaaaa aactactaag agattattaa aatatatggc
    12181 aaaacaaaag tttaaaatat taattatttt tatttcagta ttgataagtt cagcgcttac
    12241 agttttagca cctatggtta tgggaaaagc tattgaccaa ctatttaatg gaataaaaac
    12301 tgcagtccaa acaggtacaa aattcagtgt gaattttagt actatggggg ggatagtatc
    12361 aatacttcta gggttgtact taattagtgc agtatttata tatattcaac agtatattat
    12421 gtcaggagtt gcacaaaatc tagttcttag catgagaaaa gatttaagtg ataaactcaa
    12481 taaacttcct cttaagtact atgattctca taaaaagggg gaaacactta gtatagtaac
    12541 aaatgattta gaaaaagtag cagatagttt acaagaagga ttaatgcaac ttattacagc
    12601 agtagttaca gtagttggtt ctattgtaat gatgataagt ataagtgtac cacttacaat
    12661 agtatcagct ataacattac ttgtttcatt gggtattact gttgtaattg caagaaaatc
    12721 acaagaaaga ttctctgaaa atcaaaaggc actaggagag ttaaatagca atatagaaga
    12781 gatatttact ggtcaaatag tggttaaagc atttagtaaa gaaaaggata ctataagaaa
    12841 ctttaaaata ttaaatcaaa atctttataa tgcaagtaga aaagcacagt tttcgtcata
    12901 tgcaatatct ccaattatta gatttataaa cctaattggc tatgttatta tagcagttgt
    12961 aggtgggata tttgcatcaa ctggagcaat gactcttggt agtatacaag cgtttattca
    13021 atatgtaaat caagcatcag agcctactac agaaatatct tatattgtaa atatgcttca
    13081 agcagcaata gcatctgcag agagagtttt tacagttatg gacgaggtgg aagagataaa
    13141 ggatgaagaa tcttcaaagg ttattagcat gcctaaaggt aaagtacaat ttgagcatgt
    13201 taaatttgga tatagtgatg attttattct tatgaaagat attaatatca atctaaatgc
    13261 aggagataag attgcaattg ttggacctac aggagctgga aagacaactc ttgtaaattt
    13321 acttatgaga ttttatgaga tacaaggtgg aagaattact attgatggtg ttaatataaa
    13381 agatttaaag cgtggagaac ttagaactat gtttggtatg gttcttcaag atacatggct
    13441 gtttaatgga agtataaaag aaaatattgc atatagtaag tcagatgcca caatggatga
    13501 aattgtaaga gcagcaaaat cagcaagagt agaccatttt ataaggactt taccacaagg
    13561 gtatgacact atattgactg aggatgcttc taatctatct caaggtcaaa agcaacttct
    13621 aacaattgcc agggcaatac tttcagaccc atcagtattg attcttgatg aagcaacatc
    13681 tagtgtagat actagaacag aggtagaaat tcaaaaggca atgaataact tgatgaaggg
    13741 aagaactagt tttgttattg cacatagatt atcaactatt cgagatgctg atttaatatt
    13801 agtaatgaaa gaaggaacta ttattgaaca aggaagccat aaggaactta ttgaaaagaa
    13861 aggattctat gaagaacttt ataacagtca atttacaagt gaatatgatg aagatgttgt
    13921 atgattatca agttttaggc aactaaatga ttgataaata ttaagttaaa tatagtaaca
    13981 tgtaaggtta tatggaataa gagtatttct ctatttcata taaccttttt tatttatcaa
    14041 aataagataa aatttccttt ctaaattaga ttgaagaatg tatttatcaa tataaaagta
    14101 aaataatcat tattattagt gaagtaaata ttgtttgcca catatatgcc ataaagatgc
    14161 acttatttta cggagattta acaaagtagt gtaaagtata ctctaaatat aaaataaatc
    14221 aaatagagtt aaataaattg agaggtgata taaatagtga ataaaaaatt gaactataga
    14281 tttgaaatga agcacaaaat aactgaagca gatgtgttag ctttaaaatc aagactctat
    14341 caaataatga agaaagatga aaatgcatct aaagatggaa aatatcttat taggagttta
    14401 tattttgata ctcctgaaga taaagcactt ttggataaat taaatggcgt tgcaataaga
    14461 gaaaagttta ggataagatt ttataataat gactgctctt atataaggct agagaaaaaa
    14521 atcaaacatt acaacatgac atcaaagcta tcaactaatt taaccaaaaa agaagtaata
    14581 aatatactaa ataatgatat tgaatttcta aaagagtcga caaattgttt attaagagaa
    14641 ttttatttga aattaaaatg tgaaagatta gaggcaaaaa gcatagttga ttacaataga
    14701 gaagctttta tttatccagt tggaaatgta agagttacaa tcgatagtga tattaaaact
    14761 tctgtaaatt cggtagattt atttaataaa gatttgccaa ctgtttcggt aattgatgag
    14821 aatatgactg tattggaggt taaatatgat gaatttatac cagattttat aaaggattta
    14881 attcaaatta ataagactac ctctacagca gtttcaaagt atgcttcaag tagattatat
    14941 atttaagaat aaaggctatg atttatataa agatatacat aaataaggtt aaaaaacaaa
    15001 taagttaaaa ataaataaaa taaaaaataa ataagttaaa aataggaagg atgattttaa
    15061 ttatgacatt taatgatatt tttaaatcaa gttttataga aaacgtatca ggtttttcat
    15121 ttgtggacag tgctttagca ctaggttcag cttttttagt aggtttgttt atatatatgg
    15181 tctataaaaa gacttatatg ggaattatgt attctcgacc attcaatgtg tcgcttgtag
    15241 cacttacaat gcttacaact tttgtaatat tggcagttac ttcaaatgta gttttatcct
    15301 taggtatggt tggtgcttta tctatagtaa gatttagaac agcaattaaa gacccaatgg
    15361 atttagtatt tctattttgg tcattgggtt caggtatagt tttaggagca ggtttgattc
    15421 ccttagctgt catgggttca ataattatgg gattgattct aatattcttt tcaaataaaa
    15481 caatatctga aactccatat atattgatgg taaattgtaa taatgaagat tcagaagata
    15541 tagcaactga taaaattaaa aaagtattta ctaaatatca aataaaatca aaaagtgtta
    15601 caccagaaaa aggaatagaa ttagtgtttg aagtaagaat gaaagatgga gaaacaagtc
    15661 ttataaatga cttgagtcaa gtagatggag ttactaatgc agttcttgta agttataatg
    15721 gtgactatgt agcatagaga ggagtaatct tttatgaaag ataaaaaatt tacccttctt
    15781 atctcgatta tgattatatt tttatgtgct gtagttggag tttatagtac atctagcaac
    15841 aaaagtgttg atttatatag tgatgtatat attgaaaaat attttaacag agacaaggtt
    15901 atggaagtta atatagagat agatgaaagt gacttgaagg atatgaatga aaatgctata
    15961 aaagaagaat ttaaggttgc aaaagtaact gtagatggag atacatatgg aaacgtaggt
    16021 ataagaacta aaggaaattc aagtcttata tctgtagcaa atagtgatag tgatagatac
    16081 agctataaga ttaattttga taagtataat actagtcaaa gtatggaagg gcttactcaa
    16141 ttaaatctta ataactgtta ctctgaccca tcttatatga gagagttttt aacatatagt
    16201 atttgcgagg aaatgggatt agcgactcca gaatttgcat atgctaaagt ctctataaat
    16261 ggcgaatatc atggtttgta tttggcagta gaaggattaa aagagtctta tcttgaaaat
    16321 aattttggta atgtaactgg agacttatat aagtcagatg aaggaagctc gttgcaatat
    16381 aaaggagatg acccagaaag ttactcaaac ttaatcgttg aaagtgataa aaagacagct
    16441 gattggtcta aaatcacaaa actattaaaa tctttggata caggtgaaga tattgaaaaa
    16501 tatcttgatg tagattctgt ccttaaaaat atagcaataa atacagcttt attaaacctt
    16561 gatagctatc aagggagttt tgcccataac tattatttat atgagcaaga tggagtattt
    16621 tctatgttac catgggattt taatatgtca tttggtggat ttagtggttt tggtggaggt
    16681 agtcaatcta tagcaattga tgaacctacg acaggtaatt tagaagacag acctctcata
    16741 tcctcgttat taaaaaatga gacatacaaa acaaaatacc ataaatatct ggaagagata
    16801 gtaacaaaat acctagattc agactattta gagaatatga caacaaaatt gcatgacatg
    16861 atagcatcat atgtaaaaga agacccaaca gcattttata cttatgaaga atttgaaaaa
    16921 aatataacat cttcaattga agattctagt gataataagg gatttggtaa taaagggttt
    16981 gacaacaata actctaataa cagtgattct aataataatt ctaatagtga aaataagcgc
    17041 tctggaaatc aaagtgatga aaaagaagtt aatgctgaat taacatcaag cgtagtcaaa
    17101 gctaatacag ataatgaaac taaaaataaa actacaaatg atagtgaaag taagaataat
    17161 acagataaag ataaaagtgg aaatgataat aatcaaaagc tagaaggtcc tatgggtaaa
    17221 ggaggtaagt caataccagg ggttttggaa gttgcagaag atatgagtaa aactataaaa
    17281 tctcaattaa gtggagaaac ttcttcgaca aagcaaaact ctggtgatga aagttcaagt
    17341 ggaattaaag gtagtgaaaa gtttgatgag gatatgagtg gtatgccaga accacctgag
    17401 ggaatggatg gtaaaatgcc accaggaatg ggtaatatgg ataagggaga tatgaatggt
    17461 aaaaatggca atatgaatat ggatagaaat caagataatc caatagaagt tggaggtttt
    17521 ggtaatagag gaggaggctc tgtgagtaaa acaacaacat acttcaaatt aattttaggt
    17581 ggagcttcaa tgataataat gtcgattatg ttagtaggtg tatcaagggt aaagagaaga
    17641 agatttataa agtcaaaata agattaaaat tgaatataaa taattttaat actgttaagt
    17701 ctaaatattt acttagcagt attttttaga aaataatgtg tttgattaat aatataaggt
    17761 aataaattga taaaaattaa atacagcata ttaataatac ataataaagt aataaaaaac
    17821 tgtttaagta taataactta tatttagaca gtttttttca tttataaata atataaatga
    17881 aattgaaaac atttgaagga tagaaattgg ttggataaat gtataaatta cttcaccaaa
    17941 aaataatgac aaaaactgta actaagttgt aataaaatgg tagttattta gcataaaata
    18001 atgtatcata gaagtatatt ataaaattaa taaaataaga tttttgtaag aaaactgctg
    18061 gcttttgata tttagagtga gtagttaaat tatgtttagg aggagtagaa tttgttgcaa
    18121 aaagtattat ggaaattttt aaagctagta gtcggtttat ttatatgttc agttggtata
    18181 gtattgacga taaattgtaa ccttggatta tctccctggg atgtatttca tcaaggactt
    18241 tcaaatcata taggaataac tataggaact gcttctataa ttgtaggctc tatagtagtt
    18301 attgctgatg tcgttcttgg agaaaatgta ggatggggaa ctgtttttaa catgctacta
    18361 attggatttt ttatggattt acttttatat agtaatttaa ttccagaggc tgataattta
    18421 tttgtaggaa taattatgtt gatattagga ttggttcttt tgtctgttgg aatggtattt
    18481 tatatgggaa gtggtttagg aagtggacct agagatggtt tgatggttgc aattcaaaag
    18541 aaaacaggaa aatcacttaa attaataaga ggtactatag aagttggagc attaatagta
    18601 ggatttttat taggtggaaa agttggtata ggaactataa taagtgcatt tggattgggg
    18661 tatttcacac aaatggtatt tggcttattt aaattagatt gtagtaagat taaacataga
    18721 tttatagtag atgatataaa atttatcaaa gtgtatatga gtggtgagaa aaaagctgct
    18781 gttcaaaata tagttaagta atttaagaat aaattcaacg aaatgttaag tattttaaat
    18841 taacattttg tttagataga taaattgaat attaaaagaa tattaaaata tgaggattgc
    18901 tagaacacta taaaatatag atttctagca attttttatt tgttcaatat ttttttagta
    18961 ctagaagtct tattctctat ttaatgaatg aattaattta taattagaga tactaaactt
    19021 tatagatatt aatataaaca cctttaaact gtgtattttc caaagccttt aaaatgcttg
    19081 tatttattct aaataaaaaa ataaaaaaat gtcaaaaaaa ctcttgtatt atatccaaat
    19141 tttatatata attacctatg ttgtttagaa attttaaact caatgtttag taaacaaaat
    19201 gtttagtaaa ataaaacaga aactaggagg tgcaacatgg atataggtga aaagataaaa
    19261 agattaagaa cagaaaaaca actgacccag gaagagttag ctaatagatg tgaattgtca
    19321 aagggattta tttctcaatt agaaaataac ttaacatcac catccatagc tacccttata
    19381 gatatacttg aaatactagg tacaaattta agggaatttt ttaatgaaat agatgatgaa
    19441 agaatcagtt ttacaaaaga agatatgttt gaaactgaag atgaagattt aaaatataaa
    19501 ttaaagtggt taattccaaa ttcacaaaaa aatgaaatgg agccaataat aataacattg
    19561 tatccaggag gacagtataa agaagaaaag ccacatgaag gtgaagaatt tggatatgta
    19621 ttagcaggtt caatatatgt ccatataggc gaaaagaaaa ataaagtaaa aaaaggagag
    19681 agtttttact ttagaccgaa agcaaatcac tatatatcaa acgagggcaa aactacagca
    19741 aaagtgattt gggtaagtac gccaccatca ttttagaata gaggtgttta ataattggta
    19801 gaaaatataa tagaattaag ggatatatct aaacattatg aagaacttac tgttttagat
    19861 aatttcaatt tagatataaa gaaaaatgag tttctaacat tgcttggacc aagtggatgt
    19921 ggaaaaacta caacattaaa aataatagca ggatttgagt atgctgatga tggtaaagtt
    19981 ttatttgaag gtaaggagat aaataatttg ccaccatatg aaagacaagt aaacacagta
    20041 tttcaaaaat atgctttatt tccacacatg gatgtttatg aaaatgtagc ttttggatta
    20101 aaaattaaaa agactccaaa agatgtaata gatgctaaag taaaagaggt tttgaaactt
    20161 gttgcactag aaggttttga aagacgacaa gttgaatcat tgagtggagg acaacaacaa
    20221 agaattgcaa tagcaagagc tctagtcaat gaaccaaagg tattattact agatgaacca
    20281 cttggagcac ttgatttaaa gttaagacaa gagatgcaaa tagaattaaa gagaatccaa
    20341 aaaaaattag gaataacatt tgtttttgta actcatgacc aagaagaggc tcttactatg
    20401 tcagatacta ttgttgtaat gtataagggc aaaattcaac aaatgggaac accacaagat
    20461 atatacaatg aacctaaaaa ttcatttgta gctaagttta taggtgaaag taacatattt
    20521 gatggaatta tgatagaaga ttataaagtt aatttctgta atagagattt tgagtgtgtt
    20581 gataaaggat tcgaaaaaaa cgaaaatata gaagtagtaa taagaccaga agatataaaa
    20641 atggtaaagc cagaagaagg aatgttaaag gggaaagtta catctacagt gtttaaagga
    20701 gtgcattacg agatagaatt gaatgaaaat ggaagattgt ggatacttca taatactaaa
    20761 aatgctgagg ttgggactga actaggcatg gatatatatc cagaagatat ccatataatg
    20821 agaaaagaga ataactaaga tgaaaagaaa atctttttta gcatatccat atgtagtttg
    20881 gagtgcgata tttgtaataa ttcctttaat attagtagta ttttttagtt ttacaaaaga
    20941 atcaggtgga ggttatgcct ttacacttga gaattataaa gaagtaattg accctatata
    21001 tatgaaagtt tttgggcgtt ctattctttt agcaggagga gctacattaa tatgccttat
    21061 agttggttat ccagtggcat atataatttc aaaggctcgt gtaagtagaa gaggttcatt
    21121 aatattattg tttattcttc ctatgtggat gaactttctt ttaagaacat atgcatgggt
    21181 tgctatatta ggaaaaaatg gacttttaaa tacattttta ggttggtttg gaatacaacc
    21241 attagcaatt ttgtatacta attttgctat acttttaggt atggtgtata actttttacc
    21301 atttatggtt cttccaatat atacagcact atcaaagatg gataatgatt tgataaacgc
    21361 tgctcatgac ttgggtgcaa ataatatgac agtttttaga aaaattatat ttcctttgag
    21421 tttgccaggt gtaatgtctg gaataactat ggtatttatg ccagcagtca ctacttttgc
    21481 aatatcaaga ctgttaggtg gaggtaaaat aatgctggtt ggagatttga ttgaacaaca
    21541 gtttacagtt gttggagatt ggaactttgg ttcagctata tcgatattta tgatgatagt
    21601 aatattaata tctatgtcta taatgtcaaa atttggagat gaatctgaca aagaaggcgg
    21661 tgggttacta ttttaaaatt aaagaaaata acttctaata tatatttagc attagtattc
    21721 ttatttttgt atgcacccat atttgctttg gtactatttt ctttcaatga ttcaaaatca
    21781 atggcaagat ggaatggatt tacatggaag tggtatggtc aattacttca aaatgagagt
    21841 ataatgagtg ctttatatta tactattgta atagctatat tagcgtctgt aatatctaca
    21901 atagtaggaa ctataagtgc tataggaata cataagatga gaggtaagag taagaaatta
    21961 atactaaatg taaactatct tcctatttta aatacagaga tagttacagc agtagctctt
    22021 atgagtctgt ttgtatttgt aaaaatggaa tttggattta caacaatgct attggcacat
    22081 ataatgtttt gtttgccata cgttatactt tcagttttgc caaaaatgaa gcaattacct
    22141 gataatatag aagatgcagc tatggacttg ggagcaacac ctatatacgc tcttagaaaa
    22201 gtaattttac cacaaataaa accaggtata gtttctggat ttttaattgc atttactatg
    22261 tcaatagatg actttataat aagtttcttt aatgctggaa atggtgtaag caatctttct
    22321 atagaaatct atggaatggc tagaagaggt ataaaacctg aaataaatgc attatcaaca
    22381 ataatgtttg cagtggttct aggtttactt ttactagcta ataaaaaaga atctatagta
    22441 agggggatta aataatgatt aagtttaaaa aagcaatatc ccttatagct gtaggtatga
    22501 tgacagttac tttatttaca ggatgtaata aagcaactga ttctacaaaa gttttaaatg
    22561 tatataatgt tggagattat atagatgagt ctcttataga taagtttgaa aaagaaacag
    22621 gaatagatgt tcaatattca acttatgata ctaatgaaat gatgtatcaa aaagtaaaga
    22681 gtggtagtac acactatgat ttagtatttc cttcagacta tatggtagag aaaatgaaaa
    22741 atgaaggtct tttggaaaaa ttagatttta agaatatacc taatatgaaa tatttagata
    22801 agagtttttt aaatcctgta tacgacaaaa ctaatgaata tagtgttgca tatatgtggg
    22861 gaactcttgg aatattgtac aataagaaag aagttaaaga ccctatggat agctggaata
    22921 tactttggaa tccaaagtat aagggaaata taatgatgtt tgactctgtt agagatacta
    22981 tagggataac tcttaaaaaa ttaggatata gcatgaatag tgtaaatcca aaagaaataa
    23041 atgaagctaa aaatctttta atgaaacaaa aggatttagt attggcttat gtaaatgatg
    23101 aaggtaagga tagattgctt ggtggagaag tggcaatggg tatgctgtat tctggagatg
    23161 cagttacact aatggagcaa aatcctgacc ttgattatgc tatacctaaa gagggaacta
    23221 ataaatgggt agatgctatg tgtgtaccta caacagcaca aaataagaaa gaagctgagc
    23281 tgtttataaa ctttttatta gacccagaga atgcaaaagt aaatgctgag tacattgggt
    23341 attcaacacc taatacagga gcactaaaat tattagaccc agaaataact cagaatcctg
    23401 ttgcatatcc atctaaagaa gtacttgata aatgtgaaac ttttgaagat attggtgaaa
    23461 atataaaatt atatgacaag gcttggatag agttaaaatc aaaatagggt cttgttgttt
    23521 taagtaaatt tacttaataa atacttaata aaactgttct tttttagaaa atttattttc
    23581 taattattga acagttttat tttttgttta agttatattc atatgcaatt taaatagttt
    23641 attaataaat gaaaacttat ttgtataatg actttaataa taattagaaa aaatgggcaa
    23701 cttaatagaa taattggagg tgtaaattta taaaaaatga ataggataaa taaaaaaata
    23761 gatttattaa tattggcttt gctcgttttt atatttttaa ttgttggaat agtaatacta
    23821 tcgattagga cagaaaataa attgaatgtg ttcataatgt tagcaataat tttttttata
    23881 ataatgttaa cttatttatc aaattcagta gtggggttga taacaagttc aataattata
    23941 tttatgtata catcctatat tttatataac aatattactc acaatatgga tgttgaattt
    24001 atatcatata tgtggataat agcaacacca gtatcttcaa ttataatggg taatctgaac
    24061 aaaagcataa atgaattgca aaatacaaat aaaaaactat ctgaacagta taaggaatta
    24121 gtgactattg atagtgaaac tggacttaga aatttaaaga ttttttataa tgatgtaaat
    24181 atggagataa gtaaatctat tagacataat actgattttt ctcttatgat agtaaaatta
    24241 ccatactacg gaaacctaca gactatattt ggtgaaaaca aaactaataa aatagtaaaa
    24301 tatattggtt caaatattat agaatgtaca agaaatgaag atataattta tagtttacaa
    24361 aaagatatga tagggatact aatgccaaat acatctttgg aaggttcaaa agttgttaaa
    24421 gacagaataa agaaaaggat aaaagaatta aatttagatt taaacaatag gggaaaatat
    24481 gtaaatatag atgtcaaaat tgcattttta caatataaga gtagttttgg agatagtata
    24541 aattttaaga atatagttga agaggaactt caatatgatg tttaaaatat ttaataaaag
    24601 tctattaaaa ttagtagttg tattatcaat agtactgaca atatcaaatt ttagcttatc
    24661 ttcatatgca tcagaaatta cttccagcac agataaggta ttaatcatat atgattctaa
    24721 gaaagaaaca gcttataata gagatatttt aaatattatg aggactctat tgggaagatt
    24781 tagttcagat atagagctat taaaattgag taactatgat ggtgagataa ataaaaatta
    24841 ttacagtcat atatttattt taggaatcaa tgaaaactca tataataatg ataaaaacac
    24901 taaaaacttg attagttcct taaattcata taaaggaact atatgttggt taggatatgg
    24961 tatagaaaat cttttggaac ataaaaaata caatttagac tatgtaggaa aaacaaacaa
    25021 tatagttagt gtaaattata gaggtaaatc atataacttg gatgaacatt atgtttttaa
    25081 cattgtagaa tcaaaagata caagtaataa agtaatagga agtattaatg ataccttaaa
    25141 taagtatcca tatataataa atgataaaaa tcttttttat gtatcaaaat tagatttaga
    25201 tggagtttta ttttatatat tttgcgattc acttaatgat atttttaata ttaagacgtt
    25261 tgataaagga cgtatatttg taagaataga agatgtacat gcttttagag aaccaaaaaa
    25321 tcttgtagag atagcggatt atttaagtag taagaatata ccatttacga ttgcattaat
    25381 accagcatat gtaaatccta aaaatcataa ggtaataact ttatcagaaa gccctgaaat
    25441 tgtcaaagca ataaaatata tgcaagataa aggtggaact gtgatattgc atggatatac
    25501 tcatcaatac aagaaagaag aagtttctgg agaagggtat gaattttggg atggtaaaaa
    25561 agatgaaccc ttaaaagaaa atatgaagat atttgtcaaa gatagagttt tgaatggtct
    25621 tagagtatgt atagagaatg gaatttatcc tcttgcattt gaagcgccac attatgcaat
    25681 ggaatcagag ggatataaag aattaaaaaa atatttttct acttatatgg ggcaacatca
    25741 aaataatgat aaaaaattta gcacaaatac atatccttat attattaggg atacagagga
    25801 gtttaatata tttatacccg aaaatttagg atatatagac ccagaagata agtttacttt
    25861 tcaaaatata aaagaaaatt taaataaatt atccattgta agaggtttta gtggagggtt
    25921 cttctttcat tcatatttaa acatagagta tctaaaaaac acaatagaat atttagaaaa
    25981 acagaatata gaatttatga atttaagaga ttttaataat tgggtaaaag ttgatgaaat
    26041 acaaataaga aataatggag atgaaattat agtcaactat gataaggact tagatgaaat
    26101 aactaaaagt gatactagat ttaaatccat atccaatatt agtaaagttt taatattcat
    26161 agtatctata agtgttttaa tatttgtgat tatatttata tattttaaga gaatagataa
    26221 aaagaaattt ttaaaataaa tgattttata ttagaatcaa tattttaaaa tattttagga
    26281 gaaatttatg agagatttaa atgtatcaga gtatttgttc gtatttagtc tgtttagtat
    26341 atggtcttta ttgcttataa atattatttt agctatgggt ggatatatat tttattttaa
    26401 aaactttgat aaagaaataa aagaaattga tgaatatcct atgatatcaa tacttgttcc
    26461 tgctcataat gaagctaaag tgataggtag aactgtggag tctttgctat tactcaatta
    26521 tcctaagagt aagatggaac ttattgttat aaatgacaat tcttctgata atagtaaaga
    26581 aattttagaa aatataaagg ataggtacaa caattataat ttcactataa taaatacaga
    26641 tagtttaaca ggtggaaaag gaaagtctaa tgcattaaat attggatata cgatttcaaa
    26701 aggagatttt atagctgttt atgatgcaga taacacacca gataaaaatg cacttagata
    26761 tctagtacaa actatagtaa tgaatgatga attaggtgct gttattggga aatttaggac
    26821 tcgaaataaa aataaaaacc tattaaccaa atttataaac atagaaacat taagctttca
    26881 atggatgtca caagctggta gatggcaact ttttaatcta tgtacaatac caggaactaa
    26941 ctttatattg aggagaagta tcatagaaga aattggagga tgggatagta aagcaattgc
    27001 agaagataca gaaataagtt ttaggatata taaattggga tacaagataa agcttgtacc
    27061 acaatctata acatgggaac aagagccaga aactgttaag gtgtggataa agcaacgtac
    27121 tagatgggca aaagggaata tatatgtatt aatgaaatat ataaaaaata tatttaaaca
    27181 gggaagaaat aagattgtgt ttgatatagc ttactttttt tctgtatatt ttctattttt
    27241 aacatctgta attatatccg atattttatt tgttttaagt atatctaaac tagtagaaat
    27301 ttctattcca attaactttt ttctaatatg gatactatct tatcttttat ttattataga
    27361 agtgagtata agccttacta tagaaaaagg tgaagcaaca attgaaaata tattcatagt
    27421 ggcaataatg tattttacat actctcaact atggctgttt gtagctataa aaggaatgat
    27481 agagtatcta aaagatataa tttttaaaag agaggttaaa tggtataaaa cagaaagatt
    27541 ttgattcatg tacaggagga attaacatga aaaaatttat tatatcaatt atatctttag
    27601 tattgttttt ttcaaatata tcacttatat acaaagtaaa tgctgatgag actaaagtta
    27661 aaaattataa gtttgaaaga gatataacta ttgatggtgt tataggaagt aatagtactt
    27721 tttttgaagt aaataaaaat tgggatattg aagaagtttt attgcatctt aattttagta
    27781 agagccaaat attaaatgga gatgtttcaa gtttaactgt attgataaat aatgtaccaa
    27841 taaaatctat taaattaaat gctaagacaa attataaaaa tacattagaa gtattagttc
    27901 caaaagatta tataattcaa ggatataatg aaataaaaat aaaaacatac aaaacaatat
    27961 ctgataaaat ttgccaagat gattccaata ctgggaactg gatggttatt cacaaagaat
    28021 catatatatc aattagatat aagcagaaaa aggtggaaaa ctcaataaat gaatatccgt
    28081 atccatatgc agaaattgaa aataatcata aattagatac aactatagta gtaccagata
    28141 atatgactag aggagagact acagcagtgt ttaatttggc atcagcattt ggaaaaataa
    28201 caaaaaatga tgatttaaag ttagatgtta aattatattc agaaatgaaa aattggtcag
    28261 atgataatat tatatatatt ggtaaacctg aaaatacagc agaagaaatt ttggatattt
    28321 taagtataaa ggaacaaacc ctattatctt caaactgtat aatcaagcaa gtagattcac
    28381 catacaataa gaataagaag atgatggtgg taattggtag taatgaagat gatttaataa
    28441 aggcatcaaa tttattaata gaaaatagac tttctaacca agttttatca tcatcagtac
    28501 ttgtaaacaa agagactaat ataaaaatta atagggaaca aaaattaaat ttaggtcatt
    28561 taacattaaa agacttagga tattctgact ttttgttgga aggagcattt aatcaacaag
    28621 ctttgtttga tgtcaaaata ccaacgggta aggtattaga tgatggttca aagattatct
    28681 taaatttaag atattctgac aatttagatt ttgaaaaatc tttagttaca gtgtctataa
    28741 atgatgttat tgtgggaagt aaaaaattgg ataggtcaca ttctaataat gataagttag
    28801 aattaaaaat acctaaagat atagataata aaaactatta tcaagttaaa ttgacattta
    28861 atttgagtat aaaaaattcg aattgtgtta ctagagagag taataatcct tgggcatatg
    28921 tatcaaataa ttcttatttg gcgttatcta tgaaagaaaa tgaaacctta tcttttgaaa
    28981 attatccgta tccatttgtt agagatgatg aatttaatga tttaactgtg ataatgccag
    29041 attattcagg gtcacaagct atgacttgga tgtttagatt aggtgttact ttaggtgcga
    29101 atataaattc acataatgga aatataaatg taattagagg aaaagaattt agtgacaagt
    29161 ataaggatac taatattgta gtttttggag tgccacataa taattctgtt ataaagatgt
    29221 taaataataa tttaaatata aaattcgata aaaattactc caattttata tcaaatgata
    29281 agataagctt tattgatgat tatggaaaaa atatttctac cattcaatta ataaaatcac
    29341 catataataa tcaaaaaaat atcatggtaa ttagttcaat gaatgaaaaa aacttatatt
    29401 taggaatgga ttatttgttg aataaaagta aagttaatga tttaaaagga gatacattga
    29461 taattgatga atacggagag gtagaagatt tagcttataa tttaaaatct aagaaagaag
    29521 taaaagattc aagttggaat atgagtataa ataagacaac taaagtattt ttaatgattt
    29581 cttttattac tataattgta gtcatgatat tatcaatgct atatataaaa aaatataaaa
    29641 gaagataata tgataaattt attattttaa aagaggtata taatttgagg aaatatatga
    29701 ataaatttaa attaaaaaga agtattttaa tctcattaat aatcttaatt tttatattta
    29761 tagtttactt tgtaagtatt gagttaaaag ataggagtct agtaaataat caatataaag
    29821 gtaagattaa atcggccaat ttatctgtag attatgaaat aaatcaagtt atgaaggata
    29881 tagataaatt gggattgaat acagtaaatg tacctataat tataaatgta gagtccatta
    29941 attctgatat tatgtcaata gataataata gtaaagaaaa ggcaattaag ttaataaaaa
    30001 aattaaataa aaaaggtatt tcaacaatat tagaggcata tccatggatt gaaaatggaa
    30061 aattgtatga gactgactgg aaccctataa ataaaaaaag atttttttat acttggcaaa
    30121 atgccatatt aaatgaactt ataattgatg tagcaaatcc actggatgta aatgttttaa
    30181 atattggttc taattttgta catttggaag aatatcaaca aaattggggt gaaataatag
    30241 attttgtaca atccaaattt gacggttttg taacgtacag aactaattgg tggtatacaa
    30301 aaaaagatga tttaagtagt aagttgtttt atgaacaaaa gctgaacaat aatttttttg
    30361 ataagttaga ttttatatct atagcagcat attttgagtt aagtaataaa cctgttaata
    30421 cagtggatga attaatttct gccttacact catctactgt aaacaataga ggacaaaata
    30481 taaaacaaga aatatataac ctttataaag ctcatagaaa acctatattt tttggagaat
    30541 taggtttttc aaatagagaa agtgcaagtt ctcagccgtg gaatcatact ccttcaaaag
    30601 ctgtaaatgg cgaagaacaa gcaaggtgtt ttgaggcata taagaaagtt tttgaaaatg
    30661 aagactggat aaatggtttt tctgtgtttt gtgtaggtaa aatagatgac gaaaaaaatt
    30721 tttacccatc aaaagagagt ataaaagtta ttaagtcttg gtatgaataa ttttgaaagt
    30781 gtagaattta tatagattaa atagatgatt aggagagaca taaatgaatg acatcaaggt
    30841 tatgactgta tttggaacta gacctgaagc tatcaaagta gcacctttaa taaaggaact
    30901 tgaaaaaaga gagaatataa aaagtattgt ttgcgttaca gctcaacata gagaaatgct
    30961 tgaccaagtt atagaaacct ttaatataaa tgtagactat gatttggata taatggaaaa
    31021 aggacaaagc ctgaatgata taacatgtaa gattttaaat aaacttccct tgattttaaa
    31081 caaggaaaat ccaaatataa tattggtaca tggagataca accacaactc tagctacaag
    31141 tttgactgca ttttataata aaactcttgt tggtcatata gaagctgggc ttagaaccta
    31201 tgataaatat tctccttttc cagaggagtt aaatagacaa cttacaggta ttattgctga
    31261 tatgcacttt gctcctacta atttggctag aaaaaacttg atttctgaag gaaaacctaa
    31321 taataatata tttgtaactg gtaatactgc tattgatgct ttaaagatga caataaaaga
    31381 aaactataat catcctataa ttgatgagat tggtaacgat aggatgattt tactaacctc
    31441 tcacagaaga gaaaatttgg gtaaacctat gaaaaatata tttagagcta taaagcgaat
    31501 agtagatgac tttgaagatg tacagatagt atatcccata catttaaatc ctaaaatacg
    31561 aactatagct gatgagatat ttggaaaatt tcctgaaaaa attcatatta tagagccttt
    31621 agatgtagct gattttcata actttttaaa taaatcttat atgataatga ctgatagtgg
    31681 aggaatgcag gaagaagctc catcattagg taagccagtc cttgtgttgc gtgataaaac
    31741 agaaagaaca gaaggaattg aagcaaagac acttaaatta gtaggtacta atgaagatag
    31801 aatatataat tcagtgagcg atttattaat aaataaagat aactatgttc agatgagtaa
    31861 agcatctaat ccatatggag atggaaatgc aagcaaatat atagtggata taataattaa
    31921 aaaatttaat tgcaaatatt taaattagat aagacctaaa acattaacta attgatataa
    31981 gatttatgat aatattaagg aatacaattt ttaaggggga gtaatatggc tagaaaatta
    32041 ataaaaaatt tgggtaaaag taaaagtgta aagagagtga aacttttatt taagaagatt
    32101 tttattactg tattcatagt agcaagtata gttgctattt ttaatataac gaaatatttt
    32161 gaggaattat acaaagtaag ggatttaaaa agtactaaaa ttgaatacta tatggatgtg
    32221 gctgatgaag caggagatgg aaaggtccaa ttaagttgga aggccttact tgctatagac
    32281 atggtaatac atgatgaaga tttaagtaat ataaaaaaga aagacacatt ggatataggg
    32341 gaaaagttta tagtagaaga taaaaatgat aaaggcgaaa aggtgtataa ggtaaaaaag
    32401 tttaataagg tattaagcga attgaaattt gactcttctc aaaaaagtag agcaagaaaa
    32461 tacatgaaag atttagaata cacatacctt ggaaataaac aattagatag tagtgatgaa
    32521 aaaattaaat ttataaagaa gttagaagac tcagctataa gagaatatat tgattatgga
    32581 atattgccct ctataacaat tggacaagct atattagaat ctggttgggg aaattctaaa
    32641 cttacaaaac agagtaataa tttatttggt ataaaagcag ataaagcatg gaaaggaaaa
    32701 agtgtagaaa tttcaacttc agagcattat aatgaaaaaa ttgtagctag ttttaggtcg
    32761 tataattcat tacaagattc tgtcaaagac cacagtttat ttttaattaa taataaaaga
    32821 tatagaaaac atggattgtt tgaggcaaaa gattatatta gtcaagctca atcattagaa
    32881 aatgcaggat atagtacagc agaagataaa aaaggaaatc gtatatatgc agagctatta
    32941 atagatgtta ttaggagtta taatttgcaa ttgatagata ataaagttga gacaaaataa
    33001 ttgttaactt tatatgagga gataacaaga taagattctt ttctacaaaa agaatagaaa
    33061 aattgtaaaa aaaactaaaa aaatgtattg acttgttatt taaaaggtga tattattatt
    33121 actgtactcg aaagagaaca aaa
//
LOCUS       DAEPIN010000046        32562 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.046, whole genome shotgun sequence.
ACCESSION   DAEPIN010000046 DAEPIN010000000
VERSION     DAEPIN010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 32562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32562
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.046"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            <1..405
                     /locus_tag="KR250_002333"
     CDS             <1..405
                     /locus_tag="KR250_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HBH0068264.1"
                     /translation="YNKGEYSYIYENKREESEIIKYYNKTDLLYLKNVVSNIVPSRDE
                     FITIYKQALIKKEIDLDMVNIRETFNVIPLKFFTILNVFRELNLLDFNLNYEKNSVLI
                     RILPKPQKKLDLNESLILNNLKNLEKQYNSSY"
     gene            418..930
                     /locus_tag="KR250_002334"
     CDS             418..930
                     /locus_tag="KR250_002334"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HBH0068265.1"
                     /translation="MDLKNFIRNIDDFPKPGIDFKDVTTLFKDGDAFKYAVDSIVEEL
                     KDKDVDLVIGPEARGFLMGTPVAYALGVGFVPIRKPGKLPGEVESYEYGLEYGTDTLE
                     IHKDAIKKGQKVAIVDDLLATGGTMEAAAKLVEKLGGEVVSMQFLIELKFLNGREKLS
                     NYDVNSLIKY"
     gene            1004..3211
                     /locus_tag="KR250_002335"
     CDS             1004..3211
                     /locus_tag="KR250_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003229747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HBH0068266.1"
                     /translation="MHDKELQEIIEKIKVYAPNANFEMVEKAYNLAKKAHEGQYRKSG
                     EPYFIHPLAVANILIEMELDIETITAGLLHDVVEDTEYTYEDIKKEFGKEVADLVDGV
                     TKLGQIKYRSKEETQSENLRKMFLAMAKDIRVILIKLADRLHNMRTLKYMPPEKAKYK
                     AKETLEIYGGIAHRLGISKIKWELEDRALRFMDPEGYYDLVSRVSMKRSQREDYIQGI
                     VELLKDKFKDLSIPCDVYGRPKHFYSIYRKMQKKHKTFEEIFDLTAVRILVDNVKDCY
                     AVLGIVHTLWRPIPGRFKDYIAMPKPNMYQSLHTTVVGPDGEPLEIQIRTHEMHNIAE
                     NGIAAHWKYKEGISSKESKVEEKLQWLRQMMEWEKDLKDPQEFMDALKEDVFNSQVYV
                     FTPKGDVIELPAESTPIDFAYRVHTNVGNKCVGAKIDGRIVPIDYKLQNGNIVEVLTS
                     SNSNGPSRDWLNIVKTPNAKSRIRQFFKKERREENVDRGNTILEREFKKHDIPQKDPL
                     VEKYMALVAKKFNQPSVEDLIATVGYGGIMSSQVVSKVKDLYLKEVKKAKKEQQIQEE
                     DLNKHNISETEYSKKRKKNASQGIIVKGLDNILVRFAKCCNPLPGDEIVGYITKGRGV
                     AIHRADCPNANNNGDFFQNRLVEVSWSSAGKGKFEAEIQIKAIDRRGIVNEITHIVAM
                     DKLSLNGINARKGKDSIVSINLLVEVNDTEELKILMKKLKAIPGVESIYRMTN"
     gene            3236..3685
                     /locus_tag="KR250_002336"
     CDS             3236..3685
                     /locus_tag="KR250_002336"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HBH0068267.1"
                     /translation="MRAVVQRVSSSKVTVDENTIGQINKGLLVLLGVTHDDKSSDVDY
                     MIDKILNLRIFEDENDKMNLSLMDIGGELLVVSQFTLYGDCRKGRRPGFSNAARPELA
                     NNLYEEFVKKAKDKGVTVGTGQFAAHMMVELTNDGPVTILLDSSKSF"
     gene            3700..4314
                     /locus_tag="KR250_002337"
     CDS             3700..4314
                     /locus_tag="KR250_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBH0068268.1"
                     /translation="MIIKKFVDSYFGVNTYVLGDEKTKKCAVIDPGGSLVETLSFVKE
                     NELNIEYIILTHGHGDHIGYVKDIKEKTGAKVVAHVDEKELLNDKNKNLSYTMRCGAQ
                     EFDADIYVNDKDKLELGELKMTFLHTPGHTQGCMCIRVEDEMFTGDTLFAGSIGRTDL
                     YSGDFNQMEKSLKKLCKYEDAVRVYPGHGPTSTLGVEKKTNPYM"
     gene            4405..5961
                     /locus_tag="KR250_002338"
     CDS             4405..5961
                     /locus_tag="KR250_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase"
                     /protein_id="HBH0068269.1"
                     /translation="MLYVFLKGHDYKYEVAEFIKLFTSEFKFKDSNANNNIESKHLIN
                     RLIYENGVLFSSTEYYENGNLKYESIQNIENMNLEFLDGFDSSKTIEEFLNSLDSESK
                     RNFKKLCKENIKKSMFLVLKQVFNSYVPWGILTGIRPVKIVHNLMDKKLDDNSIRTIL
                     KDNYFIIDEKIDLALEIAKRERLFMYPIDKNKISLYVSIPFCPTRCVYCSFPSNSLKQ
                     FGHLKREYVYKLIEEIKGFAKVIKDTKKEVETLYIGGGTPTTLDEEELDLLINSLFNE
                     LDLSKIKEFTVEAGRPDTITEQKLRVLKKHNVSRISINPQTMNDDTLVKIGREHRVSD
                     LVDCFNMARRMGFDNINMDIILGLIDEDLNMVKNTLEEIKKLSPESLTVHTLAIKRAS
                     NLNINMDRYKEHLTQYEEMVKMIDLSMKYAKDMGLNPYYMYRQKHMLGNLENIGYAKE
                     GYECIYNIQIMEEKQSNYALGAGAISKFVYVDEDRIERVENVKNVEQYIERVDEMIKR
                     KKEEVYKNVD"
     gene            5951..7213
                     /locus_tag="KR250_002339"
     CDS             5951..7213
                     /locus_tag="KR250_002339"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HBH0068270.1"
                     /translation="MLTKAPRGTKDITPKEAYKWRYVENKFREICALYGYEEMVTPIF
                     EHTELFKRSVGDTTDIVQKEMYSFKDKGDREITLKPEGTAGVVRAFIENKLYADTQPT
                     KLFYVTPCFRYERPQAGRQRQFHQFGIEALGSDTPSMDAEIIALAVQFFNEVGLNDLV
                     VSINSVGCPVCRKEYNALLKEYLDSKADILCDTCNERREKNPMRVIDCKNPTCKENIK
                     DIPFIADHLCDDCKSHFDKLQEYLKEMNINFVIDKTIVRGLDYYRKTAFEIISNDIGA
                     QSTVCGGGRYDGLVEQLGGPKGISGIGFGLGIERLLLTLEGNGIEIENPQSTDIFIVT
                     IGEEANTRSFKLLKDLRQNHISADKDHIERSVKAQFKYSDKINSKFTIVIGDDELKND
                     TATLKNMKTSEQTTVKLSTLVEELKQKL"
     gene            7287..9074
                     /gene="aspS"
                     /locus_tag="KR250_002340"
     CDS             7287..9074
                     /gene="aspS"
                     /locus_tag="KR250_002340"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HBH0068271.1"
                     /translation="METLKGLKRTHYCGELREKNINEEVVLMGWVQKKRNLGGLVFVD
                     LRDTSGLCQIVFDTDVDKEAFEKAEKLGAEFVIAVKGKVCERQSKNPNMLTGDIEIFA
                     TELRLLNKSETPPIYIKDDDDVSEALRLKYRYLDLRKPSMQRNLKLRHKVMNITRNYL
                     SNNRFCEIETPFLIAPTPEGARDYLVPSRVNPGKFYALPQSPQLYKQLLMVSGMDRYF
                     QIVKCFRDEDLRADRQPEFTQIDCEMSFVEQEDVMSMIEGLLEAIFKEVLDVELALPL
                     PKMTYAEAMSKYGSDKPDTRFGYELTDISDVVCNCGFKVFADATQPGKSVRGINVKGK
                     ADDFTRKQISSLEEHAKTYRAKGLAWMKVGQEGVTSPIAKFFNEEEMNAILTRMNAEV
                     GDLLLFVADKNSIVFDALGQVRLEVANRLNLLDKNVYNLLWVTEFPVFEEDEETGTFS
                     AMHHPFTSPMDEDLDKLEEGDKSSLRAKAYDIVLNGYEIGGGSVRISNSDVQSRMFKA
                     LGFTEERANEKFGYLLEAFKYGTPPHAGLAFGLDRLVMLLAGTDNIREVIAFPKNQNA
                     VCPMTNAPTLAEDEQLEELSIKVDIKDNE"
     gene            9533..10765
                     /locus_tag="KR250_002341"
     CDS             9533..10765
                     /locus_tag="KR250_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HBH0068272.1"
                     /translation="MQEREELVLNPVPKEERVGWLAPLFNMLGSNIAISELMVGGTLI
                     LGMTLSNMIITSIIGNLILVAIIMIQGYIGYKEGLNTYVLAKGAFGEIGGKYLISLLL
                     GITSFGWFGVQAGVAGLSVQKIFPSVNLTLVTVILGLFMVVFALYGFKAMAKFNYLVI
                     PPLMILMVWGVFKAFSTYGVEAIYNYTPQTTMSMVEGLNIVVGLVIVGAIISPDQLRY
                     TRRVKDIWIISFLGLGIISLFQQVAAGVMSMGAPTWDITEVLANLGFGWVAFVILILA
                     SWSTNVSNAYSGGLALKTIFPNVKRNVLTLVAGLIGTIIAATGIIFKFQSFLSFLGIA
                     VPAIAGIMWCEYYFIQGRTYKHREGINWIAVISWLIGFAASYYSSKINFLIPPINGIV
                     VSMVIYYILMKCFGIKDK"
     gene            10779..11585
                     /locus_tag="KR250_002342"
     CDS             10779..11585
                     /locus_tag="KR250_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase"
                     /protein_id="HBH0068273.1"
                     /translation="MGRKVKIGIIQQHSVLGDVKKNIEKAVEMIDDLGKQGADIICLP
                     ELFATGYNLESLGGVKTLELIREHNKYIEESMSEAAKRNNVYLISPYGTLEKGSTHVY
                     NSAVIFDRKGKIMGEYCKNHLWSLEAVYFKGGEKVEVYDADFGRFGVMICYDAGFPEV
                     SRELTLKGSEIIFIPSAWRIQDEDMWDLNVSQRALENTVYTVGVNLVSNDSNLILFGK
                     SKICNPRGTVITQLDTYREKYVLSEIDLDEIKKYREDIPYLKDRKSSYNI"
     gene            complement(11697..12566)
                     /locus_tag="KR250_002343"
     CDS             complement(11697..12566)
                     /locus_tag="KR250_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBH0068274.1"
                     /translation="MDLLHLKYFQTVARMEHITKASHKLNIAQPALSKTISSLEKELG
                     VQLFDRKGRYIVLNEYGRLFLKRVDSILDLVESSKKELQDTSLENSGEVKILSPAAAN
                     VLPSLLSNFRKLYPNITFNVSHTLPSSYKKSDFDLYISSSFTKLNSENSITLTCEEIL
                     LGVSINHPLSLKDEVYLSEVSDENFIVITKGENYREVIDILCESANFKPKIAFESDSP
                     YTIYALIKSLQGVGFICGKSWGLSQDPEIKLLHIKDIEFKRYLNLSWFSENYESKAVL
                     LFKNFLINYFKNI"
     gene            12938..14365
                     /locus_tag="KR250_002344"
     CDS             12938..14365
                     /locus_tag="KR250_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp14"
                     /protein_id="HBH0068275.1"
                     /translation="MKLTKSISSMLCLSLVLSLSPVAYANEISRTTVENTLVGQDRYQ
                     TAVEVSKKGWSSANEAVVVNSESIVDALAVTPFAKLKNAPILLTEKNNLNSQTEKELK
                     RLGVKKVYIIGLSSSVSNDVQKQLESNNLTTDRVGGSDRYQTALSIAKKIEGLKDISE
                     IAVVNGYTGLADAVSIGSVAATNGMVILPVSDASGISSFKDFIESKNINKSYIIGSTN
                     AVSDKIKQSLPNSERIGGADRNETNGKVIEKFYTSNKLNNVFVAKDGMKNQNQLIDAL
                     AVGVLASKENSPVLIVGEQLSATQKSVFSNKNATSITQVGSGGNENAFKELKDLQANS
                     NNKRVMYVTNTDKVNIRNDATIEASVIGYLNNGDEVEVLDVLKTGWVKIKYNEGIGYV
                     SGSYLTNNKPDNSNENIKIKYVKEKDGLNVRKGPSTEDEKIGHLSYGSKVETIEMFAT
                     GWVKIKYNGGYGYVSNDYLSDTPVI"
     gene            complement(14442..14711)
                     /locus_tag="KR250_002345"
     CDS             complement(14442..14711)
                     /locus_tag="KR250_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0068276.1"
                     /translation="MKFEYYYLIQDIAGILLAFIGLRMSIIGFRILSMRGLSINTLLI
                     VIKYCLFTIAGLNLLISKFGIRHWIWSVCMLIISIIINPRIKVSK"
     gene            complement(14714..16156)
                     /gene="nhaC"
                     /locus_tag="KR250_002346"
     CDS             complement(14714..16156)
                     /gene="nhaC"
                     /locus_tag="KR250_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HBH0068277.1"
                     /translation="MKMNTERKIPSFRYSLLVMLAIIALTSVGIVVFNASITTMFLLS
                     WLIVVPAAMKLGYTNDEIEAFGFEVGKDAFQSNLIILSVGVLIAAWIAAGTIPTVVYS
                     GLTIITPKYFLLTTLIVCSLTSVATGTSWGTLGTSGIAMMSIGTSMGIPLGLTAGAVI
                     SGAFFGDKMSPLSDSTNLAAAVCKTDVITHMKHMFYTTGPAYVICIVLYTVIGFKYSN
                     NTIDYVQINQIKDVLNSNFHIGLVAMIPIIFLLLLLLLQKPPIISILSSAIMGLIIAV
                     FQEGEKIGDVLNYMLSGFTIDTGFVYADKLLNRGGIMSMAETVLLVFVVFVIAGILQK
                     TGFLEVLLQPLITKIGKSRTKLVGSTFIVSYFANAFSSSMMFTSVFVGTLMSPIYKEF
                     KLKPQNLSRIIEDTATLGGPLIPWNSNAVFCAQTLGVSPLEFIPYCFLSWITPIISFI
                     YGVTGFTMLTYTDDEIEELEIFDTAQRRVD"
     gene            complement(16181..17347)
                     /locus_tag="KR250_002347"
     CDS             complement(16181..17347)
                     /locus_tag="KR250_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBH0068278.1"
                     /translation="MNYNFNEIVDRSNNFSSKWSEMEKKYGTNDLLPMWVADMDFKAA
                     PCIIDSLKNRLEQEIYGYTTRPDSYNESIVNWLDRRHNWKIKSEWLIYSPGVIPAISL
                     LINELTKANDKIMIQEPVYSPFNSVVKNNNRELIISPLQKLENGNYIMDYEDIENKIK
                     DVKLFILCNPHNPVGRVWTKDELKKLGDICLKHNVKIISDEIHSDIILKKHKHIPMAS
                     ISKEFEKNTITCMAPTKTFNIAGLQSSYVILPDEKYYKLLDDAFTRIDIKRNNCFSLV
                     ATEASYNNGESWLESFLEYLESNIDFAIKYINENMPKLKVRKPEGTYLLWVDFSALGL
                     SDEELESILVQKGKVALNQGNSFGIGGSGYQRINLACPRSMLEEALIRIKNAIN"
     gene            complement(17572..19692)
                     /locus_tag="KR250_002348"
     CDS             complement(17572..19692)
                     /locus_tag="KR250_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBH0068279.1"
                     /translation="MKQMELAIVSLKKDAGEIYENQIRQFLGDNLKINLYSFEEGNLK
                     FFKEKLILLSAYLKYDEIVKLSHYDAQIIVPKLTFEKNSIDMISKLEKDKIIYVYNLS
                     KDMAIETISLIHRLGIDNINILPCYPEIEFTPTDAVILTPGEKILPKFKNCEVIDLKY
                     RIIDLSCIVEIATKTKLKHLIKDDLIKKYVEKIIPTSYSTGELLLDANKFERQFDLLL
                     SIIDDGIICTNNDGIIQFYNHMARKILSINANEMIDSFVGDCIKDINFQSILTNKTPF
                     FEKLIKINHIDINLEIKHIQLNVFDGFILKMTKFSQLEKKQAKLRAQLVNSGNISKYT
                     FDDILGSSIQTINTKKIANKMAQSNSSILIIGESGTGKELFAQSIHSASRRKDGPFVA
                     VNCSTFQENLLQSELFGYDEGAFTGAKKGGKIGLFELANNGTIFLDEIGEMDLNSQSK
                     LLRVIQEKQVRRIGSNNVIDIDVRIIAATNRNLKELVSKNMFRRDLYFRLNVLPLKIH
                     PLRERPADIFEIFGSLKYDIPCNFILSEEVKEIFKMYRWEGNVRELRNLGEYFCYLEK
                     DIIEICDLPEYILDTIDSNYSRTVCNKVSNNIKKYQFNIGKDKNIMKYDYNFKRSLDE
                     YIFILDNLKKAYDLKERIGRKSLCKIALEENRFLTEQQIRNMLLELQDFGLVDILVGR
                     GGSIITSKGVEFLKNINRSNKLNS"
     gene            19925..20326
                     /locus_tag="KR250_002349"
     CDS             19925..20326
                     /locus_tag="KR250_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0068280.1"
                     /translation="MKKCLYCNRDIKSPNNKLAIKYDDNMNIYPCRAECKEKIEEYIA
                     KKPTYKFINILEVLLGVGGIFCFLSKWTIAAQVVLGIDALVIFLFPLAGFKMNGKLSM
                     EQTKNQSRSIAISILAFIVIITVLYFKQIGK"
     gene            20369..20662
                     /locus_tag="KR250_002350"
     CDS             20369..20662
                     /locus_tag="KR250_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HBH0068281.1"
                     /translation="MEERKLAPNNEREALIKRLKRIEGQVKGIQKMVEQERYCVDILV
                     QISAIRSAINRVGTIILENHIKGCVAESIKHDDADKTEEMIKELMDTINKFTK"
     gene            20677..21117
                     /locus_tag="KR250_002351"
     CDS             20677..21117
                     /locus_tag="KR250_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HBH0068282.1"
                     /translation="MNQQGFIMEIVDDRTAKLKMQRHSACAACGKCATTNSAESKEIL
                     VEVDNTIGAKVGDHVEVSMDNMNVLKAAVMAYIIPLIALLVGTIGTYYILGAIGMTTG
                     VEAISGLVGICLTIICYLYLKKNDKKFRDSREFIPVITRILIDL"
     gene            complement(21209..21682)
                     /locus_tag="KR250_002352"
     CDS             complement(21209..21682)
                     /locus_tag="KR250_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBH0068283.1"
                     /translation="MTDMSMILHFLFSGVATAGFAVFFNAPLYLLLPAGVTGGIGWIV
                     YVYLFNFTTNAVFAGFIAAALVSACSETLARKLKQPAIVFVIPGILPLIPGIGLYNTM
                     LSLIQKNYSLAMSKGTDALFLSAAIALGVLVVTSFVRTLNLLKIRKNFIPYRKSK"
     gene            complement(21682..22470)
                     /locus_tag="KR250_002353"
     CDS             complement(21682..22470)
                     /locus_tag="KR250_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBH0068284.1"
                     /translation="MNQNEELEYKKNILKLALFVGELMIKNGAETSRVEDSVLRICRS
                     RGFYHVNIFTTPTVIIISDEKFDGFSFMKTIQSRGINLNKISLLNSFSREFVSDKEYD
                     IDNAIARLYEIQDVKPYPLWLFYSCTGIASACFACLLGGNYVLNFILTFIIAIFAAIT
                     YDKTMKISAISAFSCLVSSFLIALSGVLLVEIGILDDPKMLIVGAIMPLLPGVAFIKA
                     IRDLISGNLISGISRAFDAAMIIIAIASGVGFVLDTWYRIGGPF"
     gene            22798..24042
                     /locus_tag="KR250_002354"
     CDS             22798..24042
                     /locus_tag="KR250_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HBH0068285.1"
                     /translation="MFENLKKADPEIYESMKRELKRQQRNIELIASENIVSVPVMETM
                     GSHLTNKYAEGYSGKRYYGGCEYIDEVETLAIDRIKKIFGAEHANVQPHSGANANIGV
                     YFAMLKPGDVVMGMNLSQGGHLTHGAPVNISGQYYKFYEYGIDKDSGLIDFDEVRKIA
                     HEVKPKMIVAGASAYPREIDFKKFREIADEVGALLMVDMAHIAGLVAAGLHQNPCEVA
                     DFVTTTTHKTLRGPRGGVILCKEKYAKDIDKAIFPGIQGGPLEHIIASKAVCFKEALS
                     DEFKEYQVQVAKNAKALAEELIKRDFKLISGGTDNHLILLDLTNKNITGKAAEKRLDD
                     AYITANKNTIPFDPNGALVTSGIRLGTPAVTTRGMKEEDMAIIAEAIDLCLTYDEESK
                     ARTLVVGLTEKYPLYEEYNIMD"
     gene            24365..25461
                     /locus_tag="KR250_002355"
                     /pseudo
     CDS             24365..25461
                     /locus_tag="KR250_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422954.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-diphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
     gene            25452..26150
                     /locus_tag="KR250_002356"
     CDS             25452..26150
                     /locus_tag="KR250_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBH0068286.1"
                     /translation="MYVVGLIIVVALIFLVHSIIPTYYNKLLNKEVLKNMAGENEIAL
                     TFDDGPDKRYTEKLLDVLKENDIQAMFFVVAKNAEKEPEIIKRMLRENHIVGLHSLEH
                     RNAWLYSYSYVKKDFIESTNIMKNLGVDVNYYRPPWGHTNIFSNSFVKKYNLKMTLWD
                     VMAEDWEKDSTVDIIINKLMSRTKENSIICLHDAGENSGGAVGAPERTIEALKIAIPK
                     LKASGLKFVTPERM"
     gene            26153..27217
                     /locus_tag="KR250_002357"
     CDS             26153..27217
                     /locus_tag="KR250_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HBH0068287.1"
                     /translation="MISKTEKKNKCLGSVAFLVLLMGITGYFVFRGQSVESLIKSLKG
                     ASPMFILIGFAMMFIYVACEGINIYLGMKALNQKTTLLKCMGYAFIGFYFSSITPSAS
                     GGQPAQVYYMKKDDINISYSSLILLVIVVIHQVVILAYSGIMFIMEREFILNNVSGMN
                     ILLIYGVITNVALVIGVIAIIFSKKLVNNFIISITNLLGKLRIIKDVESSRKVIISQI
                     EEYVKGAQYIKQNPKLVVQILVITIVQITSMFLVPFFVYKAFHLSTYTVFEILAIQSL
                     LNIAVSSLPLPGAVGASENSFMTLFKIFFPGHLLVPAMLLSRGISFYAFVAISGLICI
                     VVHVKSSEKVKAVKKVAYVR"
     gene            27400..28104
                     /locus_tag="KR250_002358"
     CDS             27400..28104
                     /locus_tag="KR250_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0068288.1"
                     /translation="MNKEIPKKILVADDNPEIREIVEILLTGEGYEVIMATNGEEAVN
                     LVDSTIDLIILDVVMPVKTGFVACNEIREKTTAPILFLTAKTQDSDKVIGFSAGGDDY
                     LSKPFSYSELISRVKSLLRRYYIYQGKEKEREPNILKTKELCVNLDTQDVTLNGEPVF
                     LTTIEYSILILMLKNRKKVFSSENLYETIWDEQYFYTANNTIMVHIRNLRKKLEKDQK
                     NPQYIKTAWGKGYYID"
     gene            28097..29419
                     /locus_tag="KR250_002359"
     CDS             28097..29419
                     /locus_tag="KR250_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBH0068289.1"
                     /translation="MINKIKPSKLGVILLIYVVISAAVAFILNIPFYSAVESIVKDVS
                     EGKVYFKREVNQLMDNFQTYVNNNGIRADDGDDIAKWNSDNWFVSMKIYKGNKVFYDT
                     LYYPNEVPDKSEENEFYSSPMARNYEIQFSDIKANIVVVALFRFRFQNFIEAISITFM
                     ALTFIITFLLLFSRKIKYLRKIEMGIKIVESGSLKYRIPVKGNDELSSLASSINEMSH
                     TLENQLIKEREMKNKNKDIVTSISHDVRTPLTSVICYLDLIKDHKYNSPEQLEQYIKN
                     VRSKAYQIKDLTDDLFTHFVNSGEEVSKGYEKINANEFIFQLLLDSSYTLEEKGFHIT
                     IENELYNQYHIYVDVTQFRRIFDNLCSNIIKYARKDKPIVFLIKVEDSKLLIIQKNRV
                     SNFSKNVESFGIGIKNCEKIVAYHNGSINVNSDDKEFAIEISIPAKES"
     gene            29606..30979
                     /locus_tag="KR250_002360"
     CDS             29606..30979
                     /locus_tag="KR250_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0068290.1"
                     /translation="MVLTGAHYIYILFMVIILITMIMKKDTIVPCILGVFFMGLFFEK
                     NIFGAITAVFNSFIISLNELGPIILIIAIMVALSKALEANNAIQYMVRPFSRVIKNSN
                     TAFFVTGFVMLVLSWFFWPTPAVALVGAVFLPVAMRAGLPAIGVAVALNLFGHGLALS
                     TDFVIQGAPSITAGAAGVAVSYVINDGMILFWVMGIVTISMAFYTLKRDINKGMFREE
                     LKGFESEEVEAFNGKSKIATILVALGFLADIIAMYVFDLKGGDASALLGGTAVFLIII
                     INVINFGKDSLENVCENIIDGFVFGIKIFGAIIPIAAFFYMGEVAPLTGVFGKVLAPG
                     SQGLLSDIGIALSQTIPLNKFAVSGIETVVGAITGLDGSGFSGISLVGSLASVFGTAI
                     NASVGALAALGQISGIWVGGGCLVPWGLISAAAICGVSPIELAKRNFVPVITGLVVTT
                     IVAMFII"
     gene            complement(31351..32289)
                     /gene="pdaA"
                     /locus_tag="KR250_002361"
     CDS             complement(31351..32289)
                     /gene="pdaA"
                     /locus_tag="KR250_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-lactam-biosynthetic de-N-acetylase"
                     /protein_id="HBH0068291.1"
                     /translation="MFRKVLHYLTLLSISIFFIVGCSNSQNNQNENQNKETQLQEDKE
                     KIDSGKDTSNVIVSDGTDKPSKATTNNDNNKLDVSSLDNTTLDWFYIPNNKHKTPEVN
                     TDIEFKFSDYDALYNGPTKDGQKTLYLTFDEGYENGYTTKILDTLKQNQVKAVFFVTA
                     PYIKENKDLVKRMVSEGHIVGNHSKTHPSMPTKTSNLKNFNDELYDVEKLYKDVTGKD
                     MVKFFRPPMGKYSEKSLAMTKNLGYKTVFWSFAYRDWDTDKQPSHEEATQKIMDNLHD
                     GSILLLHAVSKTSTEILNDFISNARKLGYEFELLEY"
BASE COUNT        12332 a         3884 c         5458 g        10888 t
ORIGIN      
        1 tacaataaag gcgaatattc gtatatatat gagaataaaa gagaagaaag tgaaattata
       61 aaatattata ataaaacaga cctattatat ttaaagaatg tagttagtaa tatagttcca
      121 tcaagagatg aatttataac aatttataaa caagcactca taaaaaaaga aattgatttg
      181 gatatggtaa atataaggga aacttttaat gtaattccat taaagttttt tacgatatta
      241 aatgtattta gagaattaaa tttacttgac tttaatttaa attatgaaaa aaattctgta
      301 ctcataagaa tattgccaaa accacagaaa aaattagact taaatgagag tttaatactc
      361 aataatctaa aaaatttaga aaaacaatac aatagcagtt attaggaggc gtttcccatg
      421 gatttaaaaa actttataag gaatattgat gatttcccaa agccaggcat agattttaaa
      481 gatgtgacaa ctctatttaa agatggagat gcatttaaat atgcagtgga ttctatagtt
      541 gaagaattga aagataaaga tgtagattta gtaataggac cagaagcaag aggatttttg
      601 atgggtacac ctgtagcata tgctttagga gtaggatttg ttccaattag aaaacctgga
      661 aagttaccag gtgaagttga gagttatgag tatggattag agtatggtac agatacttta
      721 gagatacata aggatgcaat taaaaagggt caaaaagtag caatagtaga tgatttattg
      781 gctactggtg gaactatgga agccgctgcc aagcttgtag aaaaattagg tggagaagta
      841 gtttctatgc aatttttgat tgagctaaaa ttcttaaatg ggagagaaaa gttgtctaac
      901 tatgatgtta attctcttat aaaatactag tacttttata atagttggta taacaccaac
      961 tattttcata tatattaaaa ttttatacaa aataggtggt tatatgcatg ataaagaatt
     1021 acaagaaata attgaaaaaa taaaagttta tgcacctaat gcaaattttg aaatggttga
     1081 aaaagcctat aatcttgcaa aaaaagcgca tgaaggacaa tatagaaaat ctggggaacc
     1141 atactttatt catccattag cagttgcaaa tattttaata gagatggaat tagatattga
     1201 aactataaca gcaggtttac ttcatgatgt tgtagaagat acagaatata cctatgaaga
     1261 cataaaaaaa gaatttggca aagaagtagc ggatttggtt gatggagtaa ctaaacttgg
     1321 tcaaataaaa tatcgttcga aagaagaaac tcaatctgaa aatttaagaa aaatgttttt
     1381 ggcaatggcg aaggatataa gggttatact tataaaactt gctgatagat tgcacaacat
     1441 gagaacatta aaatacatgc ctccagaaaa agcaaagtat aaagcaaaag agacacttga
     1501 aatttatggt ggtatagctc atagattagg aatttccaag ataaagtggg aactagaaga
     1561 cagagcatta agatttatgg acccagaagg gtattatgat ttagttagta gagtatcaat
     1621 gaagagaagt caaagagagg attatattca aggaattgtt gaacttctta aagataagtt
     1681 taaagattta agtataccat gtgatgtata tggtagacca aagcattttt acagtatata
     1741 tagaaagatg caaaaaaaac ataagacttt tgaagagatt tttgatttaa cagcagtaag
     1801 gatattggtt gataatgtaa aggattgcta tgcagtactt ggtatagttc atacattatg
     1861 gagacctatt ccaggaagat ttaaagatta tatagcaatg ccaaaaccca atatgtatca
     1921 atcattacac accactgttg taggtcctga tggagaacct ttagagatac aaataagaac
     1981 tcatgagatg cataatatag ctgaaaatgg tatagcagcc cattggaaat ataaagaagg
     2041 tatctctagc aaagagtcaa aggttgaaga aaaacttcaa tggttgagac agatgatgga
     2101 gtgggaaaaa gatttaaaag acccacaaga gtttatggat gcacttaaag aagatgtatt
     2161 taatagtcaa gtttatgtat ttacgccaaa aggagacgta attgaattac cagcagaatc
     2221 aacacctata gattttgcct atagagttca tacaaatgta ggtaataaat gtgtaggagc
     2281 taaaattgat ggaagaatag ttcctattga ttataagctt caaaatggta atattgtcga
     2341 ggtacttaca tcatctaatt ctaatggacc aagtagggat tggcttaata tagtgaagac
     2401 cccaaatgca aaaagtagaa ttagacaatt tttcaagaag gaaagaagag aagaaaatgt
     2461 tgaccgtgga aatacaatat tagaaagaga atttaagaag catgatatac cacaaaaaga
     2521 ccctcttgtt gaaaaatata tggcattagt tgctaagaaa tttaatcagc caagtgttga
     2581 ggacttaatt gccactgtag gttatggtgg tataatgtca tctcaagtgg tatctaaggt
     2641 aaaagaccta tatttaaaag aggttaaaaa agctaaaaag gaacaacaga tacaagaaga
     2701 agatttaaat aaacacaata taagtgaaac agaatacagt aaaaagagaa agaaaaacgc
     2761 atcacaagga attatagtaa aagggcttga caatatctta gtaagatttg ctaagtgttg
     2821 taatcctctt ccaggagatg aaattgtagg atatattact aagggtaggg gagtagccat
     2881 acacagagct gattgcccaa atgcaaacaa taatggtgat ttcttccaaa atagattggt
     2941 ggaggtttca tggagttcag caggaaaagg taagtttgaa gcggaaattc aaataaaagc
     3001 tattgacaga agaggaattg ttaatgaaat aactcatatt gttgcaatgg ataaacttag
     3061 tttaaacgga ataaatgcaa gaaaaggtaa ggatagtatt gtaagtataa acttacttgt
     3121 agaggttaat gacacagagg aattaaaaat tctaatgaaa aaattaaaag caattcctgg
     3181 tgtagaaagt atttatagaa tgacaaatta gagttaaaat aatagggagg aaactatgag
     3241 agcagtagtg caaagagtat cttcatctaa agttacagta gatgaaaaca ctataggtca
     3301 aataaataag gggttactag tattgttagg agtgactcat gatgataaat caagtgatgt
     3361 agattatatg attgataaaa tattgaatct gagaattttt gaagatgaaa atgacaagat
     3421 gaatttatct ctaatggata taggtggaga gcttttagta gtatctcaat ttactttata
     3481 tggagattgc agaaaaggaa gaagacctgg atttagtaat gcagctagac cagaacttgc
     3541 aaataacctt tatgaagaat ttgtaaaaaa agcaaaagat aaaggtgtga cagttggtac
     3601 aggtcaattt gcagctcata tgatggtaga gttaactaat gatggtccag taacaatact
     3661 attagactca agtaaatctt tttaaggggg aaaactttaa tgattataaa aaaatttgta
     3721 gatagttatt ttggagtaaa tacttatgta ttaggtgatg aaaagactaa aaaatgtgca
     3781 gttatagacc caggtggaag tttagtagag acattaagtt ttgtaaaaga aaatgaatta
     3841 aatattgaat atattatcct tactcatggg catggagacc atataggata tgttaaggat
     3901 attaaagaaa aaactggtgc taaagtagtt gcacatgttg atgaaaaaga attattaaat
     3961 gacaaaaata aaaatctaag ttatacaatg agatgtggag cgcaagagtt tgatgcagat
     4021 atctatgtaa atgataaaga caagcttgaa cttggtgaat taaaaatgac tttcttgcat
     4081 actccaggac atactcaagg atgtatgtgt ataagagttg aagatgaaat gttcacagga
     4141 gatactttgt ttgctggaag tattggtaga actgatttat atagtggaga ctttaaccaa
     4201 atggaaaaat cacttaagaa attatgtaaa tatgaagatg ctgttagagt atatcctgga
     4261 catggaccaa ccagtactct aggggttgaa aagaagacaa atccttacat gtaattttat
     4321 attagtattt aaaaaagtat aaaatattta ataactatat tagtgagggg gttcttctac
     4381 tgttaaatag aaggagttaa gttgatgttg tatgtttttt taaaaggaca tgattataag
     4441 tatgaagtag cagaatttat aaaattgttt actagtgaat ttaagtttaa agactctaat
     4501 gctaacaata atatagagtc aaagcattta ataaatagat tgatttatga gaatggtgta
     4561 ttattctcaa gtactgaata ctatgaaaat ggaaacttaa agtacgagtc aatacagaat
     4621 atagaaaata tgaatcttga atttttagat ggatttgata gcagcaaaac gattgaagaa
     4681 tttcttaata gcctagatag tgaaagtaag aggaacttta aaaagctgtg taaagaaaat
     4741 atcaaaaaaa gcatgttttt agtgcttaaa caagtattta attcttatgt accatgggga
     4801 attttgactg gtattagacc agtcaaaatt gtccacaatt taatggataa aaagctagat
     4861 gataatagca taagaaccat tctaaaagat aattatttta taatagatga gaaaatagat
     4921 ttagctcttg aaatagctaa aagagagcga ttatttatgt atcctataga taaaaataaa
     4981 atttctcttt atgtaagtat tccattttgt ccaactagat gtgtatattg ttcatttcct
     5041 tcaaattcat taaaacaatt tggacattta aaaagagaat atgtttataa attgattgaa
     5101 gaaattaagg ggtttgccaa agttattaag gatactaaga aagaagttga aactctatat
     5161 attggtggtg gaactccaac tacacttgat gaggaagaat tggatttgct tataaattca
     5221 ttgttcaatg aattagattt aagtaaaatt aaagaattta ctgtagaagc aggtagacct
     5281 gacactatta cagaacaaaa actaagagtg ttaaaaaaac acaatgttag tagaattagt
     5341 ataaatcctc aaactatgaa tgatgatacc ttggtaaaga ttggaagaga gcatagagta
     5401 tctgatttag tagattgctt taatatggct cgtagaatgg gatttgataa tataaatatg
     5461 gatataattt taggtcttat agatgaagac ttaaatatgg taaagaacac attggaggag
     5521 ataaaaaaac tttctcctga aagccttaca gttcatacat tagccataaa gagagcatct
     5581 aacttaaata taaatatgga taggtataaa gaacatctaa cacaatatga agaaatggta
     5641 aaaatgatag atttatcaat gaaatatgca aaagatatgg gattaaatcc atattatatg
     5701 tacagacaaa aacatatgtt gggaaattta gaaaatatag gttatgctaa agaaggatat
     5761 gagtgcattt ataatataca gattatggaa gaaaaacaaa gtaactatgc attaggtgca
     5821 ggtgctatat cgaaatttgt ctatgtagat gaagatagaa ttgaaagagt agaaaatgta
     5881 aaaaatgttg aacaatacat tgaaagagta gatgaaatga taaaaaggaa gaaagaagag
     5941 gtatataaaa atgttgacta aagctcctag aggaactaaa gacataactc caaaagaggc
     6001 ttacaaatgg cgttatgtag aaaataaatt tagagaaata tgtgctttat atggatatga
     6061 agaaatggta acacctatat ttgaacatac agaacttttc aagagaagtg ttggagatac
     6121 tacagatata gttcaaaaag aaatgtattc ttttaaagat aaaggtgata gagaaattac
     6181 cctaaagcca gaaggtacag caggtgtagt aagagcattt atagaaaata aattatatgc
     6241 agatactcaa ccaacaaaat tattttatgt gacaccttgt tttagatatg aaagaccaca
     6301 agctggtaga caaagacaat ttcaccaatt tggaatagaa gcacttggaa gtgatactcc
     6361 ttcaatggat gctgaaataa tcgcactagc tgttcaattt tttaatgaag ttggattaaa
     6421 tgatttggta gtgagtataa attcagttgg ctgtccagtt tgtagaaaag agtacaatgc
     6481 tttattaaaa gagtatcttg actcaaaagc tgatatattg tgtgatactt gcaatgagag
     6541 aagagaaaaa aatccaatga gggttataga ctgtaaaaat cctacatgta aagaaaatat
     6601 caaagatata ccatttattg cagaccattt gtgtgatgat tgtaaatctc actttgataa
     6661 acttcaagaa tacttaaagg aaatgaatat aaactttgta attgataaaa caatagttag
     6721 aggcttagat tactatagaa aaactgcttt tgaaattata tctaatgata taggagctca
     6781 aagtactgta tgtggtggag gaagatatga tggtcttgtt gaacaattag gaggaccaaa
     6841 aggtataagt ggaattgggt ttggactagg aattgaaaga cttttactta cacttgaagg
     6901 taatggaatt gaaatagaaa accctcaatc tacggatata tttatagtaa ctataggtga
     6961 agaagcaaat acaagaagct ttaaattgtt aaaagacttg agacaaaatc atataagtgc
     7021 agataaagac catatagaga gaagtgttaa agcacaattt aaatattcag ataaaataaa
     7081 ttcaaaattt acaatagtaa ttggagatga tgagcttaaa aatgacactg cgacattaaa
     7141 aaatatgaaa acatcagagc aaactactgt aaaattaagt acattagttg aagaattaaa
     7201 gcaaaagctg taaaataaga aagtatgatg ttttgtgtat tagataaata tatagcatta
     7261 aaattaaata aaatggaggt agtatcttgg aaactttaaa gggtttaaag agaactcact
     7321 actgtggaga gttgagagaa aaaaacataa atgaagaagt cgtacttatg ggatgggtac
     7381 agaaaaaaag aaatttaggt ggtcttgtat ttgttgattt aagagataca agtggattat
     7441 gtcaaatagt ttttgataca gatgtagata aagaagcttt tgaaaaagct gaaaaattag
     7501 gggctgaatt tgtaatagct gtaaaaggaa aagtatgcga aagacaatct aaaaacccta
     7561 atatgctaac tggtgatata gaaatatttg caactgaact tagactttta aacaagtcag
     7621 aaactccgcc tatttatata aaagatgatg atgatgtttc tgaagcacta agattaaaat
     7681 atagatactt agatttaaga aaaccatcta tgcaaagaaa tttaaaatta agacataaag
     7741 taatgaatat tacaagaaat tacttatcaa acaatagatt ttgcgaaata gaaacaccat
     7801 ttttaatagc tccaacacca gaaggagcaa gagattatct agtaccaagt agagttaatc
     7861 caggaaaatt ttacgcatta ccacaatcac cacaattata taaacaatta ttgatggtaa
     7921 gtggtatgga tagatatttc caaatagtta aatgctttag agatgaagac ttaagagcag
     7981 atagacaacc agagtttaca caaatagact gcgaaatgtc ttttgttgag caagaagatg
     8041 ttatgagtat gattgaaggt ttgcttgaag cgatatttaa agaagtttta gatgtagaat
     8101 tggcacttcc acttcctaag atgacttatg cagaagctat gtctaaatat ggttcagata
     8161 aaccagatac tagatttgga tatgagttaa cagatatatc tgatgttgtt tgtaactgtg
     8221 gatttaaagt gtttgctgat gctacacaac caggaaaaag tgttagaggt ataaacgtaa
     8281 aaggcaaagc tgacgacttt actagaaaac aaatatcatc actagaagaa catgctaaga
     8341 catatagagc aaaaggtctt gcttggatga aagtaggaca agaaggagta acttctccaa
     8401 tagcaaaatt ctttaatgaa gaagaaatga acgctatact tacaagaatg aatgctgaag
     8461 taggagattt acttttattt gtagctgata aaaactcaat agtatttgat gctttaggac
     8521 aagttagact tgaagtagca aataggctta atctattgga taaaaatgta tataacttac
     8581 tatgggtaac tgagttccca gtttttgaag aagatgaaga aacaggcaca ttctcagcaa
     8641 tgcatcaccc atttacatca cctatggatg aagatttaga caagttagaa gaaggtgata
     8701 agtcatcttt aagagcgaaa gcttatgata ttgtattgaa tggttatgaa ataggtggag
     8761 gaagtgttag aatatcaaat tctgatgttc aatcaagaat gtttaaagca cttggtttta
     8821 cagaggaaag agctaatgaa aagtttggtt atttattaga agcatttaaa tatggtactc
     8881 ctcctcatgc tggtctagct tttggtcttg atagacttgt tatgttactt gcaggaactg
     8941 ataatataag agaagttatt gcattcccta aaaatcaaaa tgctgtttgc cctatgacaa
     9001 atgctcctac attggcggaa gatgagcaat tagaagaatt aagtataaaa gttgatataa
     9061 aagataatga ataatatgta gttgcaagaa actaaaatgt aaaaagtctg gatataaaat
     9121 atatttatat tcagactttt tttattagaa ttattttata ttttttctct atatattttc
     9181 ataaattata cttatattct taaccaatat aattaaaata ctatgtttaa aaattttaca
     9241 taaacttgat tattttagaa actacttctg taatgagtac acgattttaa ttaaagaata
     9301 tataacaaat ttgaaataag tctaatatat gacttaatag attaaattaa aataataaat
     9361 ttggactatt tatctattta taacttaaat tatatcaaat tgataacaaa ctagatatta
     9421 tacttttttg tagaatagaa ttacaattat ataaagaaaa attctattaa tagtgaaaaa
     9481 aaagtagaaa aaagtagaaa ttattctttt ataaagaaag ggagggataa tattgcaaga
     9541 gagagaagaa ttagttttaa atccagttcc aaaagaggaa agagttggat ggttagctcc
     9601 attgtttaat atgcttggtt ctaacatagc tatatctgaa ctaatggttg gaggaacttt
     9661 aattttagga atgactcttt ctaatatgat tattacatca attataggga acttaatatt
     9721 agttgcaatt attatgatac aaggttatat aggttacaaa gaagggttaa atacgtatgt
     9781 attagctaag ggggcatttg gagagatagg aggaaagtat cttatatctc tattacttgg
     9841 tataactagt tttggatggt ttggtgtaca agcaggggtt gcaggactat cagtacaaaa
     9901 gatatttcca agtgtaaatc taacattagt tacagttata cttggattat ttatggttgt
     9961 ttttgcactg tatggcttta aagctatggc taaatttaat tacttagtta taccaccact
    10021 tatgatatta atggtgtggg gagtttttaa agctttttct acttatggag ttgaagctat
    10081 ttataattat actcctcaaa caactatgag tatggttgaa ggtcttaata ttgtagtagg
    10141 tcttgttata gttggtgcaa ttatatcacc tgaccagtta agatatacga gacgagtaaa
    10201 agatatttgg attatcagtt ttttaggtct agggattatt tcattattcc aacaagttgc
    10261 agctggtgta atgtctatgg gagcacctac ttgggatata acagaagtat tggctaattt
    10321 aggttttgga tgggtagcgt ttgttatttt aatacttgca tcttggtcta caaacgtatc
    10381 aaatgcttat tctggtggat tagcattaaa aactattttt ccaaatgtaa aaagaaatgt
    10441 tttgacttta gttgcaggtc taataggtac aataatagca gctactggta taatatttaa
    10501 atttcagtca tttttatctt tcttgggaat cgcagttcct gcaatagctg gaattatgtg
    10561 gtgtgagtat tattttatac aaggaagaac ttataagcat agagaaggaa taaattggat
    10621 tgcagtaata agctggttaa taggttttgc agcgtcgtat tatagttcaa aaataaattt
    10681 cttaatacct ccgataaatg gaattgtagt gagtatggtt atttattata tattaatgaa
    10741 atgttttgga atcaaagata aataaagggg gcaattacat gggtcgtaag gtgaaaatag
    10801 gtataattca acaacacagt gtacttggag atgttaaaaa aaatatagaa aaggcagtag
    10861 agatgataga tgacttagga aaacaagggg cagatataat ttgtttacca gaactatttg
    10921 ctacaggata taatttagag tctttaggtg gagttaaaac attagaatta ataagagaac
    10981 ataataagta catagaggaa tcaatgtcag aagctgctaa aagaaataat gtatacctga
    11041 tatctccata tggaacctta gaaaaagggt ctacacatgt ttataattca gcagttatat
    11101 ttgatagaaa aggtaagatt atgggagaat actgtaaaaa tcatctgtgg tcattagaag
    11161 cagtatactt taaaggaggg gaaaaagtag aggtatatga tgctgatttt ggtagatttg
    11221 gtgtaatgat atgttatgat gcaggttttc cagaagtatc cagagaattg acattaaaag
    11281 gttctgaaat aatatttata ccatctgcat ggagaataca agatgaagat atgtgggatt
    11341 taaatgtatc tcaaagagct ttagagaaca ctgtatatac agtaggtgta aatttagtaa
    11401 gtaatgactc taatttaata ctatttggaa agagtaaaat atgcaatcct agaggtactg
    11461 taataactca attagataca tacagggaaa aatatgtatt atcagagatt gatttagatg
    11521 aaataaaaaa gtatagggaa gatatcccct atttgaaaga cagaaaaagt agctataaca
    11581 tataattcta tactataata caagttcaat ataaaatgat aaaaagagga tgtatcaata
    11641 ttaagattaa atttattata ctttgatata tcctctttaa tattatcaac tttgatttaa
    11701 atatttttaa aatagttgat aagaaagttt ttgaacaaca acacagcttt agattcatag
    11761 ttttcgctaa accatgaaag atttaaatat cttttaaatt caatatcttt gatatgtaac
    11821 aacttaatct cagggtcttg agataatccc catgattttc cacatataaa tccaactcct
    11881 tgaagtgatt ttataagagc ataaattgta tatggtgaat cagactcaaa tgctattttg
    11941 ggtttgaaat tagctgattc gcacaggata tcaattacct ctctataatt ctcacccttc
    12001 gttataacta taaagttttc atctgaaact tcgcttagat atacttcatc ctttaatgat
    12061 aaaggatggt ttattgaaac tcctaatagt atttcttcgc atgttaaagt tatgctattt
    12121 tctgagttta gctttgtaaa tgaagaagat atgtacaaat caaaatcact ttttttataa
    12181 gatgatggca aagtatgtga cacattaaaa gtaatattgg gatatagttt tctgaaatta
    12241 ctcaaaagtg atggtaacac attagccgct gcaggagata atatctttac ttctcctgaa
    12301 ttttctaaag aagtgtcttg taactccttt tttgaacttt ctactaaatc taaaatgcta
    12361 tctactcttt ttagaaacaa tcttccatac tcatttaaaa caatatatct tccttttctg
    12421 tcaaataact gaactccaag ttctttttct aaggaagata tagttttgct taaagctggc
    12481 tgtgctatat ttaacttgtg tgatgctttt gttatgtgct ccattctagc aacagtttga
    12541 aaatacttaa gatgcaataa atccatagta taaaatacac ctcttttaat atgacttgag
    12601 ttatatcaat tttataatca actccacatt atacattata atataaagtt tatataataa
    12661 actacaatat attaaaaagt tatttaaatt gttatttata ttttgtacat aatctaatta
    12721 aaaaaagtaa ctttaaatga ttaactaagg aaatgttgtt acaaacttta gttatataaa
    12781 aaattcattt gatgagcaaa gtactaatat caagtaagaa taaaagctta ttagataaga
    12841 aaaataatat tttataataa tgatgttact taaattatta tgttatacta ttttaaatag
    12901 gaaaaattta gatactataa ttttaggggg agtcaagatg aaattaacta aatcaatatc
    12961 atcaatgctg tgcttatctc tagttttaag cttatcacca gtagcatatg caaatgaaat
    13021 aagtagaaca acagttgaga atactttagt aggtcaggat agatatcaaa ctgctgttga
    13081 agtaagtaaa aaaggatgga gtagtgcaaa tgaggctgta gttgtaaata gtgaatctat
    13141 tgtagatgca ttagctgtaa caccatttgc taaattaaaa aatgctccta tattattgac
    13201 agaaaaaaat aatctaaatt cacaaactga gaaagaatta aaaagattag gagttaaaaa
    13261 agtttatata attggtctat ctagttctgt atcaaatgat gtgcaaaaac agttagaatc
    13321 taataattta actactgata gagttggtgg tagtgataga taccaaacag ctttgagtat
    13381 agctaaaaaa atagaaggat taaaagatat atcagaaata gctgtagtaa atgggtacac
    13441 aggtcttgca gatgcagtta gtataggttc tgtagctgct actaatggaa tggttatact
    13501 tccagtatct gatgcatctg gtatatctag ctttaaagat tttatagaat ctaagaatat
    13561 aaataaatca tacataatag gctctactaa tgctgtatca gataaaataa aacaaagttt
    13621 acctaattca gaaagaatag gtggagcaga tagaaatgaa actaatggta aagtaataga
    13681 aaagttttat acatctaata aattaaacaa tgtatttgtg gctaaggatg gaatgaaaaa
    13741 tcaaaatcaa cttatagacg ctctagcagt aggagtacta gcatcaaaag aaaattctcc
    13801 tgtactaatc gtaggagaac aattaagtgc aacacaaaag agtgtatttt cgaataaaaa
    13861 tgctacctct ataactcaag ttggtagtgg aggaaatgaa aatgcattta aagaattaaa
    13921 agatttacag gcaaatagta acaataagag agtaatgtat gtaactaata cagataaggt
    13981 aaatataaga aatgatgcta caatagaagc ttctgttata ggatatctaa ataatggtga
    14041 tgaagtagaa gttttagatg tgttaaaaac tggatgggta aaaataaaat acaatgaagg
    14101 tattggatat gttagtgggt cttatttgac aaataataag cctgataata gtaatgaaaa
    14161 tataaaaata aagtatgtaa aagaaaaaga tggtctaaat gttaggaaag gaccaagtac
    14221 agaagatgaa aaaataggtc acctttcata tggaagcaaa gtagaaacaa tagaaatgtt
    14281 tgctactgga tgggtaaaaa taaagtacaa tggtggatat ggttatgtaa gtaatgatta
    14341 cttatctgat acaccagtta tttaattata agtaacaata aaaaaactgc tttaatttgt
    14401 aaagcagttt ttttataaaa atttaagtct aataactatt tctattttga taccttaatc
    14461 ctgggattta ttattatgga tattatcaac atacaaacac tccaaatcca gtgtctaatt
    14521 ccaaacttag atattaataa attcagtcct gctatggtaa ataagcagta tttaattaca
    14581 attaaaagtg tatttataga aagccccctc atagaaagta tcctaaatcc aataatagac
    14641 attctaagtc ctataaatgc caataatata cctgcaatat cctgaattaa gtaatagtat
    14701 tcaaatttca tattcaatca actcttctct gagcagtatc aaatatttct aactcttcaa
    14761 tttcatcatc tgtgtatgta agcatagtaa aaccagttac accataaata aatgaaatta
    14821 taggtgtaat ccaacttaag aaacaatatg gaataaattc tagtggactt acaccaagag
    14881 tctgagcaca gaaaacagca tttgaattcc atggtattaa tggaccacca agtgtagctg
    14941 tatcttctat aattctagat agattttgtg gttttaattt aaattctttg tatattggag
    15001 acattagagt tccgacaaat acagaagtga acatcattga tgaactaaat gcatttgcaa
    15061 agtagcttac tataaaagta gacccaacca atttagttct tgattttcct attttagtta
    15121 taagaggttg aagcagtact tctaagaatc cagttttttg gagaatacca gcaataacga
    15181 aaactacaaa tactagtaaa acggtttcag ccatactcat tattccacca cgatttaata
    15241 gtttgtcggc atagacaaat ccagtatcta tagtaaatcc tgatagcatg taatttaaaa
    15301 catcacctat tttttctcct tcttgaaata ctgctataat aagtcccatg atagctgaac
    15361 ttagtataga tataatgggt ggcttttgta gcaataaaag taataataaa aatattattg
    15421 gaatcattgc tactaaacca atatgaaagt ttgaatttaa tacatccttg atttgattta
    15481 tttgaacata gtctatagta ttatttgaat acttaaatcc aattactgta tacagaacta
    15541 tacatattac gtatgcaggt cctgtggtat aaaacatatg cttcatatgt gtaattacat
    15601 ctgtcttaca aactgctgca gctaagtttg tagaatctga tagaggtgac atcttatctc
    15661 caaagaaagc accagaaatg actgcaccag ctgttaaacc aagaggaata cccatactag
    15721 taccaatact catcattgct ataccagaag ttccaagagt cccccaagat gtaccagtag
    15781 caacagaagt taatgaacat acgattaatg ttgtaagcag aaaatattta ggggttatta
    15841 ttgttagtcc actatatact actgttggta ttgtaccagc agctatccaa gctgctatta
    15901 gtacaccaac agaaagtata attaagtttg attgaaaggc atctttgcca acttcaaatc
    15961 caaaagcttc tatttcatca ttagtataac caagtttcat ggctgcagga actactataa
    16021 gccatgaaag taaaaacatt gtagttatag aagcattaaa tacaactata ccaactgatg
    16081 ttagagctat tattgcaagc attactaaca aggaatatct aaatgaaggt atttttcttt
    16141 ctgtattcat tttcatagtg caaattctcc ttaaatattt ttagttaatt gcatttttta
    16201 ttcttataag agcttcttca agcatacttc ttggacaagc taagtttatt ctttgatacc
    16261 cacttcctcc aatgccaaag ctgtttcctt gatttagagc aactttacct ttttgtacaa
    16321 gtatactttc tagttcctca tcacttaatc caagtgcact aaagtctacc cataataaat
    16381 aagtaccttc tggttttcta actttaagtt ttggcatatt ttcatttata tattttatag
    16441 caaaatctat attagattct aaatattcta aaaatgattc tagccaagat tcaccattat
    16501 tatatgaagc ttcagttgca actaagctaa agcaattatt tctctttata tcaattctgg
    16561 taaaggcatc atctaacaat ttatagtatt tttcatcagg tagtattaca tatgatgatt
    16621 gaagaccagc tatattaaaa gttttagttg gagccataca agtaattgta tttttttcaa
    16681 attctttaga aatagatgcc ataggaatat gcttatgttt ttttaatata atgtcactat
    16741 gtatttcatc agatatgatt tttacattat gcttaagaca aatatctcct agttttttta
    16801 attcatcttt tgtccatact ctaccaactg gattatgagg attacaaagt atgaataatt
    16861 ttacatcttt aatcttgttt tcaatgtctt cataatccat tatgtagttt ccattttcta
    16921 atttttgtaa tgggcttata attagttctc tattgttatt ttttacaaca ctattaaaag
    16981 ggctgtaaac tggttcttgt atcattattt tatcatttgc ttttgtgagt tcatttatta
    17041 aaagacttat agctgggata acacctggac tataaattaa ccactcactt tttattttcc
    17101 agttatgtct cctatctagc cagtttacta tagattcatt gtaagaatct ggtcttgttg
    17161 tgtagccata aatctcttgt tctagtctat tttttaaaga atcaattata caaggtgcag
    17221 ccttaaaatc catgtcagcc acccacatag gaagtaaatc attagttcca tacttttttt
    17281 ccatttctga ccatttagat gaaaaattat tgcttctatc aacaatctca ttaaaattat
    17341 aattcattgt aaatacctcc agtaatttta attttatgtt aataatatta agcaaacaac
    17401 gtgccaatat tgataataaa aaatatttat tcacaagtta atatttaata ttaagtaatt
    17461 tcagagcata gataagataa aaatattaat tatacttaaa actttgtgga aaaggttatt
    17521 tctaataaat ttttaaatat atgaaaatgt aattaaatag gttaaatagg tttaactgtt
    17581 taacttgtta gacctattta tattttttag aaactctact ccctttgatg ttataattga
    17641 acctccacga ccaacaagta tgtctacaag cccaaagtct tgtagttcta aaagcatatt
    17701 tctaatctgt tgttctgtca aaaatctatt ttcctctaaa gctattttac ataagctttt
    17761 tctacctatt ctttctttta aatcataagc cttttttaaa ttatccaata taaatatgta
    17821 ttcatctagt gaccttttaa aattatagtc gtatttcatg atgtttttgt ctttgccaat
    17881 gttgaattga tactttttta tattattact tactttatta caaacggttc tactataatt
    17941 tgaatctata gtatctaata tatattcagg taaatcacat atttctataa tatctttttc
    18001 caagtaacag aaatattctc ctaagtttct taattctctt acatttcctt cccatctata
    18061 cattttaaag atttctttta cttcctcact tagaataaaa ttacatggaa tatcatattt
    18121 taatgaacca aaaatttcaa atatatcagc aggtctttcc ctaagtggat gtattttaag
    18181 gggaagaaca ttcaacctaa aatataaatc tcttctaaac atatttttag atacaagttc
    18241 ttttaaattt ctattagtag ctgcaattat tcttacatca atgtcaatga cattattaga
    18301 accaatacgt cgtacttgtt tttcttgtat gactctaagt aatttagatt gagaatttaa
    18361 atccatttct ccaatttcgt ctaaaaatat agttccattg ttggctaatt caaataaacc
    18421 aattttccca ccttttttag caccagtaaa agccccttca tcatagccaa ataattcact
    18481 ttgcagtaga ttttcttgaa aagtactaca atttactgcg acaaaaggac catcttttct
    18541 cctagaagca ctatgaatgg attgagcaaa taattcttta ccagtaccac tttcaccaat
    18601 tataaggata gatgaatttg attgagccat tttattagca attttttttg tattaatagt
    18661 ttgtatacta gaacctaata tatcatcaaa tgtgtattta ctgatattac cagagtttac
    18721 tagttgtgct cttaatttag cttgcttttt ttctaattga gaaaactttg tcatctttag
    18781 tataaatcca tcaaatacat ttaattgtat atgtttaatc tctaaattta tatcaatatg
    18841 atttatttta atcaatttct caaaaaatgg agttttattg gtcaatatgc tttgaaaatt
    18901 gatgtctttt atgcaatcac caacaaatga gtcaatcatt tcatttgcat ttatagacaa
    18961 aatttttcta gccatatgat tatagaattg aattattcca tcgttatttg tgcatataat
    19021 tccatcatca attatagata gtaacaaatc aaattgtctt tcaaacttat ttgcatctaa
    19081 caataactca cctgtactat aacttgtagg aattattttt tctacgtatt tttttattag
    19141 gtcatctttt attagatgtt ttaatttggt ttttgtagca atttcaacaa tacaacttaa
    19201 atctattatt ctgtatttta aatctataac ttcacaattt ttaaacttag gcaagatttt
    19261 ttcgccagga gttaggatta cagcatctgt gggagtaaac tcaatctctg gatagcaggg
    19321 caggatattt atattgtcta ttcctaatct atgtattaaa gaaattgttt ctattgccat
    19381 atctttactt aagttatata cataaataat tttatctttt tctaatttgg atatcatatc
    19441 aatactgttc ttttcaaatg tcaacttagg aacaatgatt tgagcatcat agtgagataa
    19501 ttttactatt tcatcatatt ttaaataagc agataggagt ataagttttt ctttaaaaaa
    19561 ctttaaattt ccttcttcaa atgagtataa atttatcttt agattatcac ctaagaattg
    19621 tcttatttga ttttcataaa tttctccagc gtcttttttt aaacttacaa tagctaattc
    19681 catttgtttc attttaacct ctcatacaaa tttgacttat tttagattac ataataatta
    19741 aattagatta aataatatta tttaattatt attataaatt tattatagac tacacattga
    19801 agtaaaagaa gtagctaaaa ttattccttt tatttttgtt ttttgtatct atttggtata
    19861 atagtattag agcgtataat aatttaggca aaatttttta aggaagacta gaggagaata
    19921 taaaatgaaa aaatgtcttt actgtaatag ggatataaag tcaccaaata ataaattagc
    19981 tataaaatat gatgacaata tgaatatata tccatgtcgt gcagaatgta aggaaaaaat
    20041 tgaagaatat atagcaaaaa aacctacata taagtttata aatatattag aggttctttt
    20101 aggtgtgggc ggaatattct gctttttgag taaatggact attgcagccc aagttgtact
    20161 tggaattgat gcactggtta tatttttatt cccattggca ggatttaaga tgaatggaaa
    20221 gttaagtatg gaacaaacta agaatcaaag taggtctatt gcaatatcaa ttttggcatt
    20281 tatagtaata ataactgtat tatattttaa acaaattggt aagtagagca taattgtata
    20341 agacatcatt agagtatgag gtgagtttat ggaagaaaga aaattagcac caaacaatga
    20401 gagggaagct ctcataaaaa gattaaaaag aatagaaggt caagttaagg gtatacaaaa
    20461 gatggtggaa caagaacgtt attgtgttga tatattggtt caaatatctg ctataaggtc
    20521 tgctataaat agagtaggca caattatctt agagaatcat ataaaaggat gtgtagcaga
    20581 aagcattaaa catgatgatg ctgataaaac tgaagagatg ataaaagagc ttatggatac
    20641 tataaataaa tttacaaaat aaggaggcgg ttagttatga atcaacaagg atttattatg
    20701 gaaattgttg atgatagaac tgcaaagtta aaaatgcaaa gacattcagc ttgtgctgct
    20761 tgtggaaaat gtgctactac aaattcagca gagtcaaaag aaatattagt agaagtagac
    20821 aacacaattg gcgcaaaagt tggtgaccat gtagaagtga gtatggataa tatgaatgta
    20881 ttaaaagcag ctgtaatggc ttatataata cctcttatag cacttcttgt aggaacaata
    20941 gggacttatt atatattggg ggcaattgga atgacaactg gagttgaagc cataagtgga
    21001 ttagttggaa tttgtcttac aataatctgt tatttatact taaagaagaa tgacaaaaaa
    21061 tttagagaca gtagagaatt tataccagta ataacaagaa tattaattga cctttaagat
    21121 aattataatt aaaaaatagc aatgtgcttc ttaaataaat actcaatacg tatttgtaga
    21181 gtttaagtac attgctattt ttatattttt attttgattt tctgtaagga ataaagtttt
    21241 ttctgatttt caaaagattc aatgttctta caaaagaagt tacaactaat acaccaagtg
    21301 ctattgcagc acttagaaat agcgcatctg taccttttga catagccaaa gaataatttt
    21361 tctgaatcaa agacaacatt gtattatata gcccaattcc tggtattaga ggaagaatac
    21421 ctggaattac gaacacaata gctggttgct ttagttttct tgctaaagtc tcactgcacg
    21481 ctgagaccaa agcagcagcg ataaaacctg caaaaactgc atttgtagta aaattaaata
    21541 aataaacata tacaatccaa ccaattccgc cagttactcc agctggaaga agtagatata
    21601 gtggagcatt aaagaaaaca gcaaatccag cagttgcaac acctgaaaat aaaaaatgca
    21661 aaatcataga catatcagtc attagaaagg acctcctatt ctataccatg tatcaaggac
    21721 aaaaccaact ccagaagcaa ttgcaataat aatcatagca gcatcaaaag cacgagaaat
    21781 tcctgatatt aaattccctg atattaaatc tctaatagct tttataaaag caactcctgg
    21841 gagtagtggc attatagcgc cgactataag cattttaggg tcatctaaaa tgcctatttc
    21901 aactagtaga actcctgaaa gtgcaattaa aaaagaagat actaaacagg aaaatgcaga
    21961 tattgcactt atcttcatcg ttttatcata agtaattgca gcaaagattg ctattataaa
    22021 cgtaagtata aaatttaata cataattccc tccaagcaaa caagcaaaac aagcagatgc
    22081 aattcctgta caagagtaaa ataaccataa aggataaggt tttacatctt ggatttcata
    22141 aagacgtgca atggcattat caatatcata ctctttgtca ctaacaaatt ctcgagaaaa
    22201 actgtttaat aaagaaatct tatttaagtt tatacctctt gattgaatcg ttttcataaa
    22261 cgaaaagcca tcaaatttct catctgaaat aattattact gtaggagttg taaaaatatt
    22321 tacatgataa aaccctcttg acctgcatat tctaaggaca ctgtcttcaa ccctagatgt
    22381 ttcagctcca ttttttatca tcagttcccc tacaaataga gctagtttta gaatattttt
    22441 cttatattca agttcctcat tttgattcat aaatacacct ccaaattaaa aaacgtaata
    22501 ttgaaattat atcaaatttt taagtaatat tccttaatat ctatacaaat atattatctt
    22561 ttttttgttt caaaatagtc acatttaaat tatatccaat taaaagaatt agacaatatt
    22621 atttattata ttttaactta aaaataaaga aagtaactat gttaaatatg gaaaaatggc
    22681 aaaacacttg actattaatt ggtaaaggac tataataata aattatcata ttgataaaaa
    22741 cgaaaattat gaaatgtctg taaaaataaa atttaaataa taactaggag gagtaatatg
    22801 tttgagaact tgaaaaaagc ggaccctgag atttatgaaa gtatgaaaag agagttaaaa
    22861 agacaacaaa gaaatattga attaatagca tctgaaaaca tagtatcagt tccagtaatg
    22921 gaaactatgg gaagtcatct tacaaataag tatgcagaag gttactcagg caaaagatat
    22981 tatggtggat gtgaatacat agatgaagtt gaaactcttg caatagatag aatcaaaaaa
    23041 atatttggag cagaacatgc taatgttcaa cctcattctg gagcaaatgc aaatattggt
    23101 gtatattttg ctatgttaaa accaggtgat gttgttatgg gaatgaattt atctcaaggt
    23161 ggacatttaa ctcatggtgc tccagttaat atttctggtc aatattataa attttatgaa
    23221 tatggtatag ataaagacag tggacttata gattttgatg aagttagaaa gatagcacat
    23281 gaagtaaaac ctaaaatgat agttgcagga gcaagtgcat atcctagaga aatagatttt
    23341 aaaaagttta gagagatagc tgatgaggtt ggagcattgc ttatggtaga tatggctcat
    23401 atagcaggtc ttgtggctgc tggtcttcac caaaatccat gtgaagtggc tgattttgtt
    23461 acaacaacaa ctcacaaaac tttaagagga cctcgtgggg gtgttattct ttgtaaagaa
    23521 aaatatgcta aagacataga caaagcaata ttccctggta ttcaaggtgg acctcttgaa
    23581 catataattg cttctaaggc agtttgcttt aaagaagctt taagtgatga gtttaaagaa
    23641 tatcaggtac aagttgctaa aaatgcaaaa gccttagctg aagaacttat taaaagagat
    23701 tttaaattga tttctggagg tacagataat catttaatac tattagattt gactaataaa
    23761 aatataactg gtaaagcagc agaaaagaga ttggatgatg cttatataac agctaataaa
    23821 aatacaattc catttgaccc aaatggtgct cttgttacaa gtggaataag acttggtact
    23881 ccagctgtaa ctactagagg tatgaaagaa gaagatatgg caataatcgc tgaggctata
    23941 gacctatgtt taacttatga tgaagaatct aaggctagaa cattggttgt tggtttaact
    24001 gaaaaatatc ctttatatga agaatataat attatggatt aattataaag aacaactaaa
    24061 taaaatacat ttaaataatt acaatgattg tgtatgtatt tgaatatatt tagatataaa
    24121 aaaggtgtct caaaatgaac tttttagttt atgaggcact tttttgtatg gaatcgaaca
    24181 tatttgtatt atttaaataa taaaaaatat ggttatctct atttttagat ggtctctttg
    24241 tattttttaa ttaaaacttt aagatttctt aagaattata ctaggttttt ctaaagattt
    24301 aaagtctata cttataagta tagtaatcat atgaaaagaa aagctttaac gaggagggaa
    24361 tcttatgaag gtattaattc ttacaggaaa atttggtatg ggtcattact cagcatcaaa
    24421 ttctttaagt gaagatataa aagcaaaatt tgataattca gaaataataa ttaaagatat
    24481 atttgagtac attatgccta actattcaga taaaatgtac aagacttttt ctattcttgt
    24541 aaatcgggga agtagtcttt ataatttgtt ttataaatgt gctgaaaatg gaaagaaaga
    24601 tataaaattt actttttcag attatttttt aaataaatta gatacattac ttcatgaagt
    24661 acaaccaaca gtagttattt caacttttcc attttgttct caattagtat caagatataa
    24721 ggaaaaatat aactcaaatc ttcctttaat aacatgtata actgatataa gtagtcactc
    24781 agaatggatt agtaaaaata cagactgtta tttagtagct agtaaatcta ctaaggaaga
    24841 acttgttttt aaaggaatag atgaatctaa gattaaagtt aatggtattc cagtaaaaaa
    24901 agaatttaaa agaatagaac atgtaaatca ttcaactaaa aaaatattct tataatgggt
    24961 ggaggattag gattactgcc taaatcagaa caattttaca aagaattaaa tagtttagaa
    25021 ggagtaaaaa ctactgtaat aactggtaat aataaaaaaa tgtactataa actatatggt
    25081 agatatgaaa atatagaggt agtaggttac accaatgaag tatataaata tatgaaagat
    25141 tctgacctta ttatttctaa gccaggtgga ataacactat ttgaaacaat ttattcagaa
    25201 cttccaatat tggcatttaa tccattttta caacaagaga tagataatgc tagttttata
    25261 ttaaataatg aaattgggag aatacttggt aaaaataaaa aatactatgt agatgagata
    25321 aaagatttaa tttatgatga tgctactctt aaagaaatga gcagtaacat gaaagaatta
    25381 aaaaaacaat ttgataataa taccttagag aatattttat tttcacttga tgaacaagga
    25441 gcgtgtagag aatgtatgta gtaggtttaa ttatcgttgt tgcattgatt tttttagtcc
    25501 attcaattat acctacatac tataataaac tattaaataa agaggtatta aaaaatatgg
    25561 ctggagaaaa tgaaattgca cttacatttg atgatggtcc agataagaga tatacagaaa
    25621 aattactaga tgtgttaaaa gaaaatgata tacaagctat gttttttgta gtagcaaaga
    25681 atgctgaaaa agagcctgaa attataaaaa gaatgttgag agaaaatcat atagttggtt
    25741 tacattcatt ggaacacaga aatgcatggc tttatagtta tagttatgtt aaaaaagatt
    25801 ttattgaaag tacaaatata atgaaaaatt taggtgtaga tgtaaactac tatcgtccac
    25861 catggggtca tactaatata ttttcaaatt catttgttaa aaaatataat ctaaaaatga
    25921 ctttgtggga tgtaatggca gaagattggg agaaagatag tacagtagac attattataa
    25981 acaagttaat gagtagaaca aaagaaaatt ctataatttg tttacatgat gcaggtgaaa
    26041 attcaggtgg tgcagtaggt gcacctgagc gtactattga agctttaaag atagctattc
    26101 caaaacttaa agccagtggt ttaaagttcg taacaccaga aaggatgtag ttatgataag
    26161 taaaactgaa aagaaaaata aatgcctagg tagtgtagct tttttagttt tactaatggg
    26221 cataacagga tattttgttt ttagagggca gtcagttgaa tcattgataa aatcattaaa
    26281 aggtgcaagc cctatgttta tactaattgg atttgcaatg atgtttatat atgttgcctg
    26341 tgaaggtata aatatatact taggtatgaa agctttaaac caaaagacta ctcttttaaa
    26401 atgtatggga tatgctttta ttggatttta ttttagttct attacgcctt cagcatcagg
    26461 tggtcaacca gcacaagttt attatatgaa aaaagacgat ataaatatat catattcttc
    26521 acttatacta ttagtcattg ttgttattca ccaagtagtt atactggcat atagtggaat
    26581 aatgttcatt atggaacgtg agtttatatt aaataatgta agtggtatga acattttact
    26641 aatatatggt gtaataacaa atgttgctct tgtcattgga gtaattgcaa ttatattttc
    26701 aaaaaaactt gtaaacaatt ttataatatc aataacaaat ttattaggta agttaagaat
    26761 aataaaagat gttgaaagtt caagaaaagt tataatatct caaattgaag agtatgttaa
    26821 aggtgctcaa tatataaagc agaatccaaa acttgtagtc caaattttag tcataactat
    26881 tgtacaaata acatctatgt ttttagttcc tttctttgta tacaaagctt ttcatctatc
    26941 aacatatact gtatttgaga tattagcgat acaatccttg cttaatattg ctgtttcttc
    27001 attaccattg ccaggggctg ttggagcttc tgaaaatagt tttatgacat tatttaagat
    27061 atttttccct ggtcacttat tagttccggc aatgttactt agtaggggaa taagttttta
    27121 tgcatttgta gctataagtg gattgatatg tatagtagta catgtaaaat cttctgaaaa
    27181 agtaaaagca gtgaaaaaag ttgcttatgt aagataatat tatatagatt atttttatta
    27241 atatgtcaat ttgtgtgtat attttaatta agctttaaac atgtttacag gagtagaaac
    27301 tattaaaacg tgtgacattg ctaagctttt gctaataaag tataatattt tatatagttt
    27361 gaaatattta agaaatatag caaaaaaagg agcttggtga tgaataaaga gattccaaaa
    27421 aaaattttag tagctgatga taatcctgag attagggaaa tcgtagaaat acttttaact
    27481 ggtgaaggtt atgaagttat tatggcaacg aatggagaag aagctgtaaa cttagtagat
    27541 agtacgattg atttgattat tttggatgtt gtaatgcctg taaaaacagg atttgttgca
    27601 tgtaatgaaa ttagagaaaa aacgacagca ccaatattat ttttaactgc aaaaactcaa
    27661 gactctgata aagttattgg attttcagca ggaggagatg actatttatc aaagcctttt
    27721 tcatattctg aactaatttc aagggttaag tcgctactta gacgttatta tatataccag
    27781 ggaaaggaaa aggaaagaga gccaaatata ttaaaaacaa aagagttatg tgtcaattta
    27841 gatactcaag atgttacact aaatggagaa cctgtgtttt tgacaactat agaatatagt
    27901 atattaattc ttatgttgaa aaatagaaaa aaagtttttt cgtctgaaaa tttatatgaa
    27961 actatttggg atgagcagta tttttataca gcaaataata caataatggt tcatattaga
    28021 aatttaagaa aaaagttaga aaaagaccaa aaaaatccac aatatataaa aaccgcatgg
    28081 ggaaaggggt attatattga ttaacaaaat taagccatct aagttgggag ttatactatt
    28141 gatatatgta gtaatctcag ctgcagtggc tttcatttta aatataccat tttactctgc
    28201 tgttgaatct atagtaaaag atgtatcaga agggaaagta tattttaaaa gagaagtaaa
    28261 tcaacttatg gataattttc aaacttatgt gaataacaat ggtataagag cagatgatgg
    28321 agatgatata gcaaaatgga atagtgataa ttggtttgtt tctatgaaga tatataaagg
    28381 aaataaggtt ttttatgata cattatacta tcctaatgaa gtacctgata agagtgagga
    28441 aaatgagttt tatagcagtc ccatggctag aaactatgag atacaatttt ctgatattaa
    28501 agctaatata gttgtagtag ctttatttag atttagattt caaaatttta tagaagcaat
    28561 ttctattaca tttatggcac ttacatttat tataacattt ttactgttgt ttagtagaaa
    28621 gataaagtac ctaaggaaaa tagaaatggg tataaagata gtagagagtg gcagtttaaa
    28681 gtatagaatc ccagtaaaag gtaatgatga attatcatca cttgcatcta gtattaatga
    28741 gatgagtcat acattggaga atcaacttat aaaagaaagg gagatgaaga ataaaaataa
    28801 agatatagta acttcgatat cacatgatgt tagaactcca ttaacgtcag taatttgtta
    28861 tttagatttg attaaagacc ataaatacaa tagtcctgag caattggaac aatacataaa
    28921 aaatgtaagg tctaaagcat atcaaataaa agatttaaca gatgaccttt ttacgcattt
    28981 tgttaattct ggagaggaag tttcaaaagg gtatgaaaaa ataaatgcta atgaatttat
    29041 ttttcaatta cttttagata gttcatatac attggaggag aaaggatttc atataacaat
    29101 agaaaatgaa ctttataacc aatatcatat ttatgtagat gttacacaat ttagaaggat
    29161 atttgataat ttatgttcta atataataaa atatgctagg aaagataagc ctatagtatt
    29221 tttaattaaa gtagaggatt ctaaattact aattatacaa aaaaatagag taagtaattt
    29281 ttctaagaat gtagaaagtt ttggaattgg tataaagaat tgtgagaaaa tagttgcata
    29341 tcataatgga agcataaatg taaattcaga tgataaagaa tttgctattg aaatatctat
    29401 tccagcaaaa gaatcataaa aatttaaatt atatattgtg taattgctat tatatattgt
    29461 gtaattgctt atgtaaaatt ctatgaagca ggaagcatgt gactttagtt atgggatgtt
    29521 caatattgtg tacctctagg caagtaacaa gtttacttac aagaccatat aatattatta
    29581 tcttaaaaaa aggggacata ataatatggt tttgacagga gcacattata tttacattct
    29641 atttatggta attatattaa ttactatgat tatgaaaaag gatacaatag taccatgtat
    29701 attaggtgtt ttttttatgg gattgttctt tgaaaaaaac atctttggtg ctataacagc
    29761 agtgtttaac tcttttatta tatctttaaa tgagttgggt ccaattatct tgattatagc
    29821 tattatggtt gcattatcta aagccctaga agccaataat gctatacagt atatggtgag
    29881 accattttcg agagttataa aaaattctaa tacagcattt tttgtaactg gatttgttat
    29941 gttagttctt tcttggtttt tctggccaac tccagctgtt gccttagttg gagctgtatt
    30001 tttacctgtt gctatgagag ctgggctacc agcaattggt gttgcagtag cattaaattt
    30061 atttggtcat ggtcttgcct tatctacaga ctttgttatt caaggagctc caagtataac
    30121 agctggtgca gcaggtgttg cagtatcata tgttataaat gatggtatga ttttgttttg
    30181 ggtgatggga atcgtaacca tatctatggc attttataca ttaaaaagag atattaacaa
    30241 aggaatgttt agagaagaac taaaaggttt tgaaagtgaa gaagtagaag cgttcaatgg
    30301 aaaatctaaa atagcaacta ttttagtagc tttgggattt cttgctgata taattgctat
    30361 gtatgtattt gacctaaaag gaggagatgc atcagctctt ttgggtggaa cagcagtatt
    30421 tttaataata attataaatg ttataaattt tggcaaagat agtcttgaaa atgtatgtga
    30481 gaatataatt gatggttttg tgtttgggat aaaaatattt ggagcaatta taccaatagc
    30541 agcatttttc tatatgggtg aagttgcacc attgacaggt gtatttggaa aagtattagc
    30601 accaggttca caaggtttat tatcagatat aggcatagct ctttctcaaa caataccact
    30661 taataaattt gccgtatcag gtatagaaac tgtagtggga gcaataactg ggcttgatgg
    30721 ttctggattt tctgggattt cattagttgg ttcacttgcc tctgtatttg gaactgctat
    30781 aaatgcaagt gttggtgcat tagctgcatt aggacaaatc agtggtatat gggtaggtgg
    30841 aggctgtcta gttccttggg gtcttatttc agcagcggca atttgtgggg ttagtccaat
    30901 agaattggca aagcgaaact ttgtcccagt tataaccgga ttggttgtaa caaccatagt
    30961 agcaatgttt attatttaat aaatcgaata aatctttaat tgtataaatt aaaaatagat
    31021 gtgtagctat aatttattat aattgtttta ctaattctaa gccatacatc tatttttatt
    31081 gatattttta ttagtattct ttaataaagt aatatttttc attatattat acataacatc
    31141 taaatataac atctaaaata ctgttatcta tatttaatat cttgccaaat tttttgatgt
    31201 tttattgccc tgtcccacca acatatataa gctatcacaa gtttttacct atactatcac
    31261 caaacaaaaa ttctaatttt aatttattat tttataacct atattatatt aactatatta
    31321 gtttgtagac tttagatgta attataaata ttaatactcc aagagttcaa attcatatcc
    31381 tagttttctt gcatttgata taaaatcatt taaaatttct gtactagttt tggaaactgc
    31441 atgtagtaac aagatagaac catcatgtag attatccatt attttttgcg ttgcttcttc
    31501 atgtgatggt tgtttatcag tatcccagtc tctgtatgca aaactccaaa atacagtctt
    31561 gtatcctaaa tttttagtca ttgccaagct tttttctgag tattttccca taggaggtct
    31621 aaaaaattta accatatctt tcccagttac atctttatat aacttttcaa catcatataa
    31681 ttcgtcatta aagtttttta gattgcttgt ttttgttggc atagatggat gggttttact
    31741 atgatttcca actatatgtc cctcagaaac cattcttttt actaaatctt tattttcttt
    31801 tatatatgga gctgtaacaa aaaaaactgc tttaacctgg ttttgtttta gagtgtcgag
    31861 tatttttgta gtgtatccat tttcataacc ttcatcaaat gtaagatata gtgttttttg
    31921 accatctttt gtaggtccat tgtatagggc atcatagtca gaaaatttaa attcaatgtc
    31981 cgtatttact tctggtgttt tatgcttatt gtttggtata taaaaccaat caagagtagt
    32041 gttatctaaa gaagatacat ctaatttatt attatcatta ttagtagtgg cttttgatgg
    32101 tttatctgtt ccgtctgaaa ctattacatt ggaagtatct ttaccactat ctattttttc
    32161 cttatcttct tgtaactgtg tttccttatt ttgattttcg ttttgattgt tttgtgaatt
    32221 tgaacatcca acaataaaaa aaatagaaat agaaagtagt gttaaataat gcaatacttt
    32281 tctaaacatt ttgaagctcc tttctaaatt aataataatt gcatataata ataattatat
    32341 gattaattac aattgcatat agtattatta taattggaaa ttactattaa taatagttat
    32401 atctttaaaa ataaagtcac aatagtatga caaagggtga ataatcaata aatttaaaaa
    32461 aaaatgaaaa aaatagttga ccatattatg agaagatgtt attataataa atgtgctcaa
    32521 caagaagtaa caaaataaca agcacaaaga agttaattga at
//
LOCUS       DAEPIN010000047        32540 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.047, whole genome shotgun sequence.
ACCESSION   DAEPIN010000047 DAEPIN010000000
VERSION     DAEPIN010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 32540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32540
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.047"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            482..1810
                     /locus_tag="KR250_002362"
     CDS             482..1810
                     /locus_tag="KR250_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBH0068292.1"
                     /translation="MGSNELKKTLGVSAALSTVVGSVIGAGVFFKPQAVYTLTGGAPG
                     LGILAWLIAGIITITAGLTAAEVSVAIPKTGGMMVYIKEIYGEKLGFLTGWMQIVLFY
                     PGMMAALGVIFGEQASALIGSPSLLLPIAIGIIVIVAGLNMLGSKTGGVIQTVSTICK
                     LIPLILIMVVGFIKGGGNNPILTPMVGEGLSLGSVLGQVLIAILFAFDGWMNVGTLAG
                     EMKNPGKDLPKAIIGGLSVVMAVYFIINLAYLWVLPANELANYASPASAVAEVIFGSM
                     GGKIISVGILISVFGALNGFLLTGSRVAYTLATDKTLPKYGIFSKLNSAQVPANAIAL
                     VSVIASIYALSGQFNLLTDLAVFATWIFYVLTFIGVMKLRKTHPNIPREYKVPLYPIV
                     PIIAIASGIFVVVNQLCFAGMKTTMISIGGLVITAIGLPVYAYMTRGVKR"
     gene            2563..3396
                     /locus_tag="KR250_002363"
     CDS             2563..3396
                     /locus_tag="KR250_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HBH0068293.1"
                     /translation="MRNKKKKINRKKLYLLLSAIVVCLAFIVVGVRVSSLNMKENEIS
                     RNAKLSSSTITDIVSNTSIEVSKGPSKSSGKIAYITIDDGPSKFTDEMIKTLNKYNVK
                     ATFFMIDGNMKEYPQQVKNIIKNGNTAGFHSVSHDIHKLYVTSTSAKEEFDTNDQTFY
                     KITGKHSKVIRIPYGSKPYTPQASYQALVDAGYKIWDWDLDTEDWRSNSSQIVQNVKN
                     HIKNRKGEDKDQLVVLMHEKKQSAEALDSVLKFLSDEGYEFAPVDQNQIPKNYWLRNL
                     E"
     gene            3489..4421
                     /locus_tag="KR250_002364"
     CDS             3489..4421
                     /locus_tag="KR250_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBH0068294.1"
                     /translation="MVGILSLMMVGCNKSLAKVNDVEITKEQYKKTKAVLSATNNYIN
                     GQSLDELEKTLDKKGRNKLENVIISFMVDNELLYQEAKDKGLTPSKSEVDSKYQELED
                     KMNLNTSYKEKMDKAGVDKDYLKQEISRDLAIDKNKKAFEDRINISDNDMEAYYTSHK
                     KDFNVEEVSASQILISTLDKNKKEVSKDKKEALKKKADNILTKIKNGESFESLAKKYS
                     DDKATGKNGGQLGYFTKDDKNAEFTKEVFKLKKNEVSNVFETSYGYHIVKVTDKRERQ
                     KSFNECQSLIRESILNEKYIEHIKKLNEDAKIDR"
     gene            4473..4955
                     /locus_tag="KR250_002365"
     CDS             4473..4955
                     /locus_tag="KR250_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBH0068295.1"
                     /translation="MFKSLKRTKVEKYIIDNKDSFYRIAYSYTKNEEDALDVVQEAMY
                     KALYSVENIKEVNYIKTWFYKILVRTSIDFIRKNRKYNNMTDIDLIDETGEYDKYTDL
                     DLRRALEELPIEYKSIIILRFFEDLKIEEVAIILDENVNTVKTRLYTALKKLKLKIEE
                     "
     gene            4967..5836
                     /locus_tag="KR250_002366"
     CDS             4967..5836
                     /locus_tag="KR250_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HBH0068296.1"
                     /translation="MTSKERLNKLKDEYHKIPIPKKLDTIINHEKIENIYREKNKKVN
                     RLRFKVAIAFACIFTVLVNISPVFADNFSKIPVIGAIVEVITIKNYSLKSENYEAEID
                     IPKIRGLKDKNLEQRLNSSFMEDGKRLYHQFQERMEKIQSSKNKGYKSLSLSYSVKNN
                     SKKFLSIEMTKNEIEASSYVSKVHYTIDKKRQIVLTLPMLFKDDKYIKVISDNIKEQM
                     REQMKKDSTKSYFIDQKKDLPVEDFKTINKYQDFYFNKNEDLVICFDEYEVAPGYMGA
                     VEFVIPYKVIKDL"
     gene            5879..6634
                     /locus_tag="KR250_002367"
     CDS             5879..6634
                     /locus_tag="KR250_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HBH0068297.1"
                     /translation="MTTYKKLKKSIFKIAIFSICLLVSLVDTNPIFAGSSFNETSNSD
                     TIKVLNAKSINFKQDNYEVNVSIPKIKGLKDKTLENKLNNEFLQDGKKIYSEFQNKMK
                     KENNKGHRYLNVIHNVKNNSKDFLSIEMTKEEIEASSSITKKHYTVDKNRGILLNLPI
                     FFKDSRYIRAISDNIKEQMRNQIKKDDTKSYFIDQDKDSPVKDFESINKYQDFYFNKN
                     GNLVISFDEYEVAPGYMGAVEFVIPNKVIKRLR"
     gene            6676..7497
                     /locus_tag="KR250_002368"
     CDS             6676..7497
                     /locus_tag="KR250_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase accessory factor"
                     /protein_id="HBH0068298.1"
                     /translation="MNIYEQAIRLIENNEDFAFATITSHSGSTPRETGAMMIVKNDST
                     IFGSVGGGSVEAECIKHAINVIKNRESMLYKFTLNKSDVAKLGMICGGTGEIQIDFID
                     SKLKSNIEKFNKRLKENTSKAYIFGAGHISRDVAVILSLLEFRTVVIDDREEFANHER
                     FPESEVVVLDSFENIPDFPTDENSYIIILTRGHLYDSSALEWALKREAGYIGMIGSRT
                     KIGLTYEKLMKKGFKKEELSKVHAPIGIKLNAQTPAEIAVCIAAELINCRANKEK"
     gene            7502..8263
                     /locus_tag="KR250_002369"
     CDS             7502..8263
                     /locus_tag="KR250_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HBH0068299.1"
                     /translation="MNTGSKDIDTLKVNFRGIEIKAVLVYKNRKNISIKIDPFGKITV
                     MSPPKISKKIIKDIIIEKGDWILKKSEKCKGREEVYKQRMFITGEKFLYLGEEYHLVI
                     KELLRDSVKKSNCRITINEYQIVVQTNDTSTDFIKKSLKSWYKMESERIVLERIDFLK
                     LNCEIMKQLVPASVKVKEQNKRWGSCTAQKNIYINSKISMARLDVIDYVIIHEFSHLA
                     HMNHSKKFYDLVKSIMPDYKDKENWLKINGYKIII"
     misc_binding    8424..8681
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            8794..9033
                     /locus_tag="KR250_002370"
     CDS             8794..9033
                     /locus_tag="KR250_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HBH0068300.1"
                     /translation="MENTVYAKLNQGIDSFLRVAMTLRRREVDIQSISMTVDNMNNSG
                     IKLIVNEEKTSLDSVLNHMKKLHDIREITVERMRH"
     gene            9337..10332
                     /gene="ilvC"
                     /locus_tag="KR250_002371"
     CDS             9337..10332
                     /gene="ilvC"
                     /locus_tag="KR250_002371"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HBH0068301.1"
                     /translation="MKMARMYYEKDVDLEVLKNKKVAVLGYGSQGHAHAQNLRDNGVH
                     VVIGLYDGSKSAQKAKEDGFEVKSVAEATKESDLTMMLMPDEKQKKVYEESVKDNLKE
                     GQTLAFAHGFNIHYNQVQPPEFVDVVMVAPKGPGHLVRNVFTKGSGVPALFAVYQDYT
                     KKATETVLAYAKGIGATRAGVLETTFKEETETDLFGEQSVLCGGISELIKLGYKTLVD
                     AGYQKEVAYFECLHEMKLIVDLIYEGGFERMRYSISDTAEYGDYVSGKRVITDAAKQG
                     MQNVLEDIQNGKFAKAWIKENEEGRENFLKTREEEYNTEIAEVGRNLRSMMSFLK"
     gene            10385..12073
                     /gene="ilvB"
                     /locus_tag="KR250_002372"
     CDS             10385..12073
                     /gene="ilvB"
                     /locus_tag="KR250_002372"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HBH0068302.1"
                     /translation="MSGAKVILECLKKEGIDTIFGYPGGAVIPLYDALYDYSDDFKHI
                     RTSHEQGLVHAADGYARSTNTVGVCFTTSGPGATNAITGIATAFMDSSPMVVISGQVP
                     TSLLGKDSFQEIDITGATLSMTKHNYLVRNTKELVPTIKEAFRVANSGRKGPVLVDVP
                     KDLFLAEMDFSGEDYDLCQIDDYMDYKSDFDLDDETNIKLLNEAIDIIKESKKPIIYA
                     GGGVKSSDSEEILEKFATKIDTPVLNTLMGLGNIDRKNELSLGMVGMHGSRESNLALS
                     NSDLVIAIGARFSDRVISKSSEFAKNAKIIHIDIDPSEISKNIESNVSLVGDVKLVLS
                     LLIERVESKNNSNWKEEIKRFRKSEGVQTYEFHPQNILKKINEKYETLKKPTVVVTDV
                     GQHQMWAAKYWNFKGNKSFITSAGLGTMGFGLGAAIGTKVGNVDKNVVLVTGDGSFRM
                     NCNELATVANYNVPMLILLLNNRTLGMVRQWQKLFSNQRYSQTDINENVDYVKLVNAY
                     NIDGYKVSSMEELGKALDMIDFNKPVFLQCDIDKDYDVYPIVAPNDALENLICN"
     gene            complement(12434..13564)
                     /locus_tag="KR250_002373"
     CDS             complement(12434..13564)
                     /locus_tag="KR250_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBH0068303.1"
                     /translation="MKGGVRKRSNKWYYYFDLGIVEGKRKKVERVGGNTKKEAEKALR
                     EALNEYENSGIVFEESNISLSDYLDFWYKEYVLLNCKYNTQESYRINIEKHIKPKLGA
                     YKVKALTPAILQNFINRKYKEDYSQNTLQVLKAILHRSLKSAVHPYKHIRENPMQYVS
                     IPKTKSKTETNKIKTITLEEFNQILNIFPQDSFQRIVLLIGFHTGMRRGEIIALKWDN
                     IDLDNKTITVRHTLIKKPNGMFELGQPKTESSCRTIFTGDTLIKALKEHKLYQKKMKL
                     KYGEFYFDSDWVCTKENGQQVNTHTLDTIVRQIRVALNNDFHFHSLRHAHATLLLENG
                     ANIKDIQNRLGHSQLSTTMDTYSHVTDKMKNETVDIFEKITN"
     gene            complement(13804..14310)
                     /locus_tag="KR250_002374"
     CDS             complement(13804..14310)
                     /locus_tag="KR250_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HBH0068304.1"
                     /translation="MNKLDALLDLANNEEIEIYYTDKIADDIKGLYINRQGLKIISLL
                     NSLKQNNAKLIEILAEELGHHFTSVGNYVSSKNSYKNKILIDKTENKALKWACEFLIT
                     EEEIIHVINSHATSVYEIAEELQVSINFLLKRLEFLSKKKSMLDLGNNRFLVLTNLPN
                     FYIYEDIF"
     gene            complement(14573..16162)
                     /locus_tag="KR250_002375"
     CDS             complement(14573..16162)
                     /locus_tag="KR250_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HBH0068305.1"
                     /translation="MKSIISDVINRFNATPFLFVGSGLTRRYYNLPNWEDLLKVFAEK
                     ISNDDFIYTSYKNKAKSENPKMGINPRIAELIENDFNKKWFSDSSIRSLDSKYLDIVK
                     SGVSPFKAEIAMYIKNNSKIVDKYQDEVEKLSNISKKSLSGFITTNYDCFLETIVDNY
                     TTYIGQENLVFSSIQGIAEIYKIHGCVSSPNSIVINEADYIDFDSKSAYLAAKLMTIF
                     VEFPIIFIGYSVTDVNIKKILNAIVNCLSNENAKKLEERFIFIEYEKNFNDIEISSHT
                     IVFDNKMITMTKIKLEDFNLLYEALSEKKSKLPVRILRMFKQEFYDFTITNKPTAKIR
                     VGSVDDTRIKDEDLVLAVGKASDFGLKGLKGLSFDEWYRDIVMNDLEFSSDELLEYAY
                     PSLIKQYNKLPLNKHLFNSTLEFPDYRTIALESDFENIISNSIKKNRNNTYIKNRSVY
                     GIWNDEHQSFEKATRLIAFLEEKEIDTEQLENLLKKIFEENPNILESAKTSEKTNLRR
                     LIRIYDYMKYSNMQKSLGYSE"
     gene            complement(16186..16749)
                     /locus_tag="KR250_002376"
     CDS             complement(16186..16749)
                     /locus_tag="KR250_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HBH0068306.1"
                     /translation="MSILSDRLKFLRKEKGVMQKEIANYLNITTSAYGFYEQGKRTPT
                     PEMLSSLAEYFGTTVDYLIGRYDNKASNISSKTSCNNTLFQKRLKELRAEKNMTQEDV
                     ANKLNLTKSAYGYYEQGKTVPDAYMLSSLAEIFNVTTDYLLGRSIVKNDIDTVAAHRV
                     NPHKDLPEEAQEQLNDYIEFLINKYKK"
     gene            16902..17123
                     /locus_tag="KR250_002377"
     CDS             16902..17123
                     /locus_tag="KR250_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0068307.1"
                     /translation="MNNLKELRTERNLKQKDVALKVGITTSYYGMIESGTRVPSLAIA
                     IKLSKCFGVSIEKIFLNTNTTRCCLKERS"
     gene            17126..17941
                     /locus_tag="KR250_002378"
     CDS             17126..17941
                     /locus_tag="KR250_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein/antirepressor Ant"
                     /protein_id="HBH0068308.1"
                     /translation="MKDLKIVKVNNKLTTDSRDIALMVEKEHKILLRDIRNYINQMEE
                     ANKNMSTDLYPSDYFIENTYLDDYKREKPCYAITKIGCDFIANKMTGIKGTAFTGIYT
                     KKFEEMEQVLKNEQPKLPTTYKEALQHLIEQVEVNEKLQLEGKMKDQVIKELKPKADY
                     TDMILKNKGLVTITQIAKDYGMSGKEMNKILHERGIQYKQSGQWLLYKQHQGKGYTHS
                     ETIDITRSDGMTDVKMTTKWTQKGRLFLYDLLKVNNILPDIEKEYSYQTSMLC"
     gene            complement(18120..18299)
                     /locus_tag="KR250_002379"
     CDS             complement(18120..18299)
                     /locus_tag="KR250_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HBH0068309.1"
                     /translation="MSTSLPKYTLRINRVLLEKIKYIAESEGRSANKEIEQIIKKHIE
                     DYEQRKGEIKINIEE"
     gene            18435..18614
                     /locus_tag="KR250_002380"
     CDS             18435..18614
                     /locus_tag="KR250_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0068310.1"
                     /translation="MNSNKKRITVRMPEKLNEEITKKSKYLGLTKNSFILDILWKEFE
                     LLEYRSYKKEVDKHE"
     gene            18607..19398
                     /locus_tag="KR250_002381"
     CDS             18607..19398
                     /locus_tag="KR250_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HBH0068311.1"
                     /translation="MNNLQVIERNNERVLTTQQLADVYETDSRNISNNFNNNKDKFIE
                     GKHYFLLQGDDLKNFKGIHTEYENLKFASKMYLWTERGANRHCKILDTDKAWEQFDNL
                     EETYFKVKQHKPTCIEDVLIESLKEMKDLRLQVNQANSIALEAKTEVETIKDVVSLDS
                     NSWRTNTHQLIARIAKKQGGFEHINMLRTESYELLNKRFGVDLHRRLINKRRKMAEEG
                     VSESKREKVNNLDVIQDDKKLIEGYVAIVKDMALKYGISSDLSKN"
     gene            complement(19481..19675)
                     /locus_tag="KR250_002382"
     CDS             complement(19481..19675)
                     /locus_tag="KR250_002382"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0068312.1"
                     /translation="MKFVYVKIFNNWIDITDIGSINGCNAIEYINENLDKMYSSNYVS
                     INYKDKNYCIHPSCIQIVTK"
     gene            complement(19831..20049)
                     /locus_tag="KR250_002383"
     CDS             complement(19831..20049)
                     /locus_tag="KR250_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0068313.1"
                     /translation="MTKFKDNIKLVRKQMNMTQKQFASLFGISERAYQYYEAGSREPN
                     LETLILISNKLNVSTDFLLGLSPNQNRN"
     gene            20162..20356
                     /locus_tag="KR250_002384"
     CDS             20162..20356
                     /locus_tag="KR250_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0068314.1"
                     /translation="MKINNNLKLQREKIGLTQLEVAQKAKITERSYQYYEAGERLPNI
                     RTALKIAIILNTNCEKLFNE"
     gene            20398..21192
                     /locus_tag="KR250_002385"
     CDS             20398..21192
                     /locus_tag="KR250_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HBH0068315.1"
                     /translation="MNNLQLNNKNTITTLEIADMLEIRHWEVLRKLEGTEKTKGIIDI
                     LNDNNFVVVDYFIKSTYLDSKNESRPCYNVTKLGCDFLANKFTGEKGIIFTARYVRRF
                     NEMERVLKEQQPKLPTTYKEALQQLLVEVEEKEQLQLENQEKDKVIQLQQPKVLFADA
                     VSASNNSILVGELAKLIKQNGVDIGQNRLFTWMRENGYLIKRKGEDYNIPTQKSMNLR
                     IMEVKKRVINNPDGSTKVTRTVKITGKGQVYFVNKFKSSKQLSMLS"
     gene            21249..21458
                     /locus_tag="KR250_002386"
     CDS             21249..21458
                     /locus_tag="KR250_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0068316.1"
                     /translation="MENKKIEEIEKRITMLENQLQAQLFSQITAQSNSDFIDYMYQCI
                     VLYLHEMNKSEKRKISEITKERIRK"
     gene            complement(21468..21842)
                     /locus_tag="KR250_002387"
     CDS             complement(21468..21842)
                     /locus_tag="KR250_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0068317.1"
                     /translation="MQFSNNISKELQESIRKTVSDTSNTEYFYYAEFQYKIILKSIEE
                     FEKELDDEHEIALKLTNFGKDVLMIVEEVGYHNPCLIHYYGIVNGVYSEILQHTSQIN
                     FMITSVKKTDPSKPARRIGFIL"
     gene            21918..22115
                     /locus_tag="KR250_002388"
     CDS             21918..22115
                     /locus_tag="KR250_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0068318.1"
                     /translation="MLIGDNIKQILRKRDIKPYKLAKELNIDVSGLYKLLKNKNSNPT
                     IDTLIKLADYLDITLDELVGR"
     gene            22146..22538
                     /locus_tag="KR250_002389"
     CDS             22146..22538
                     /locus_tag="KR250_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH0068319.1"
                     /translation="MDISESITKQFSDSLKSLIEIEINKQETDRVKSQTVEQKVKVLE
                     PKDIVVLIKRGYPNYLITVEEARGILKLDTVFMRRLVSTGLIKSLARGDGRKISRYEV
                     DDFIERNQGKNLDELLKVAERGDEIVKP"
     gene            22525..22668
                     /locus_tag="KR250_002390"
     CDS             22525..22668
                     /locus_tag="KR250_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0068320.1"
                     /translation="MLSLNTNKNNIVTLKKDGKAIADIVFKDIKTGKKISVGVLNKKV
                     LVK"
     gene            22835..23077
                     /locus_tag="KR250_002391"
     CDS             22835..23077
                     /locus_tag="KR250_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0068321.1"
                     /translation="MEAARLIAIGQIKQAEKEICKLQGTKNNSSLMWWEAVKFASQNI
                     LEGLEHDIELEASIEFREAMMYQEELEKDRPIDVQI"
     gene            23156..23437
                     /locus_tag="KR250_002392"
     CDS             23156..23437
                     /locus_tag="KR250_002392"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0068322.1"
                     /translation="MKTKNEIIKGLEDRLFLLRFTTVDEVDWDVKFGQISALEFCIDK
                     HRKGCTLEQFKEHLDEYKLQGNYGDYIDGFVSVLERNIREMEGEIDGSE"
     gene            23424..24062
                     /locus_tag="KR250_002393"
     CDS             23424..24062
                     /locus_tag="KR250_002393"
                     /inference="COORDINATES: protein motif:HMM:NF016302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ERF family protein"
                     /protein_id="HBH0068323.1"
                     /translation="MEVNNIYIKLMDVRIKFNKLDIKKSGQNKFANFKYFELADFLPQ
                     ATELLQEAKLCPIVTFTNDYATLTLINGEKPTEEIIFTSPMRELQLKGSNELQALGGI
                     ETYQTRYLYIQLLNITENDTFDATSGKEDYKRNDLTNSSIKASENGQIKISQNQIKRL
                     FSIGNTIGKDSDRVKSEVYYKFNKEVKDLSKQEYDQICVGYEKLQREKGIIK"
     gene            24072..24967
                     /locus_tag="KR250_002394"
                     /pseudo
     CDS             24072..24967
                     /locus_tag="KR250_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894692.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
     gene            24987..25406
                     /gene="ssb"
                     /locus_tag="KR250_002395"
     CDS             24987..25406
                     /gene="ssb"
                     /locus_tag="KR250_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBH0068324.1"
                     /translation="MNQVVLVGRLTRDPELKYIPGTGTAVASFTIAVDRNYINKEGKR
                     DTDFIPIEVIGKSAEYCANYITKGKLVALEGNIRVDNYQTQSGEKRIFTKVSTKSVQS
                     LESKNKSSNSYKESVQDGTIGLDPQGFEIIDDDELPF"
     gene            25436..25798
                     /locus_tag="KR250_002396"
     CDS             25436..25798
                     /locus_tag="KR250_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0068325.1"
                     /translation="MFKVERYFSGSVVDNLIEDDLTCRNYLALYCCLLGITKNGKKIY
                     PKPEKMLAEFGVKKDRKIKKELPVRIRNVNTGEVKEFESIDGAACFLRLKYQAVYQAI
                     KKKTKTRSGWKVEYIKEE"
     gene            26032..26883
                     /locus_tag="KR250_002397"
     CDS             26032..26883
                     /locus_tag="KR250_002397"
                     /inference="COORDINATES: protein motif:HMM:NF012372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HBH0068326.1"
                     /translation="MNVLSLFDGISCGQVAFERARIKVENYFASEIKEIAIKVAMNNY
                     PKTIQLGDVRELNVSKIPKVDILIGGSPCQNLSKGRLIANKIQDGLTGDKSNLFWEYI
                     RILKQLNPEYFLLENVVMPKKDENMISQLLGVEPIKINSNLVSYQNRDRLYWTNILNV
                     QQPKDLKISFQDYKDTSFDYCKEFEIKKTPSREKMWGNGLNGNCKNVTYEDKINCVTL
                     KQDRFSNSGLVEFGSFCRYLTTRELELAQTLPVGYTKGLSIRQAQNVIGDGWTIDVIA
                     HILSNIN"
     gene            27200..27397
                     /locus_tag="KR250_002398"
     CDS             27200..27397
                     /locus_tag="KR250_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0068327.1"
                     /translation="MNKFQKAVSQMVKQEEKENLLQGYENCIVGRSISSSIRRYVKGF
                     EKFGYSVHEVYEFINDINKYE"
     gene            27424..27840
                     /locus_tag="KR250_002399"
     CDS             27424..27840
                     /locus_tag="KR250_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003366126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HBH0068328.1"
                     /translation="MSKYNNKKIVIDGIKFDSKDESEYYLYLKEKKENGEIKDFGLQQ
                     KFELQPKFKKDGKSYRAITYTIDFAIYKWNGEVVYIDVKGYSTQQGELRKKLFDYKYQ
                     DKKLIWIAKSKKYGVDGWIEYSELKKKRKENKKKVA"
     gene            27859..28359
                     /locus_tag="KR250_002400"
     CDS             27859..28359
                     /locus_tag="KR250_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0068329.1"
                     /translation="MASKVKKEFFMATKKHLENYKQLHINIESLKLQIKNLKEFHLGD
                     FMQGLSYDSIPISKTNSISNQVENELINLEEKIIEKQIELYEMEALKYTIDVSISNLK
                     PIHKQIIRYRYIEGLEWSLIVDKVYLEERQLRERANQAISSISIALFGKKALIEQEPL
                     FKMLDL"
     gene            28781..28987
                     /locus_tag="KR250_002401"
     CDS             28781..28987
                     /locus_tag="KR250_002401"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0068330.1"
                     /translation="MNRFEGIYSFAEATKLWDLKDSTLRKAVATGKLVENIDCKKFGR
                     DWVVTIEAMEREYGKLNNIMKDLD"
     gene            29476..30663
                     /locus_tag="KR250_002402"
     CDS             29476..30663
                     /locus_tag="KR250_002402"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0068331.1"
                     /translation="MLNEKNLKNIKNILQMVQMKHLKDFENWGINKRKKETAINSLIN
                     IIQNDGEKLDYFKEWISIFTIDGHNNYYVFNYNDSDLELEDLKNIVEDIVSENLLEID
                     TSDLEETKVIYKKNDVINKRLIIRFISPALISCKNENENGNIESGLEKTFYFATLFLD
                     LSLKQIIVSIPHTVGIKSIDNIEAKARDFSEIAKYYVSKLEDLLTTYNIQIEKYNDWI
                     YDATYELAEEGSAHNNPEINKKYEENIDKIDEFAKKIAIASGITDKAIIENFKEGVGI
                     LYENILIDEFGVVEDENQYSTFVQNGDGVNSYCRVGSKTATLKSGKGHAIAKTSRNNE
                     DVKNLGVMKSFNGTLSRFLIEVLDKELYLIRTDTSKFIEERVIYDVIRKVGEYKYTFR
                     KSE"
     gene            30608..30994
                     /locus_tag="KR250_002403"
     CDS             30608..30994
                     /locus_tag="KR250_002403"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0068332.1"
                     /translation="MMLYEKLENISILLGNQSKAKSIIEELEKLMKSDIITPRLLARK
                     TKVTLLEATRVLEFLVKENELEFFIVVECSNPDSVYENEIGHYKYFTSIKEFNAFSKG
                     QECPICGCGYKYNFEDTKIGFRRLEK"
     gene            30999..31769
                     /locus_tag="KR250_002404"
     CDS             30999..31769
                     /locus_tag="KR250_002404"
                     /inference="COORDINATES: protein motif:HMM:NF016362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HBH0068333.1"
                     /translation="MSLDTLKEYLSICDDNLIWEPFYDDSMLLDEFSNYIKEIELSKE
                     WNKSKNHEKGKILEQLVRFIMSRFTILSDISVNKSTTDNELDVFVKFNDNIPIPFLCN
                     IKSKIVCECKNWKSRKVDVGMVSKLAEICNKNNAGLGIFISLNGLTGKGWQYAEGKRR
                     KLYLSDKMPIISFTVDEITKLKYKRYNLLTMIKCKMQALIDEMEFAGGELRLLESELG
                     FIDCLNENIESLKMLGLITEVEYNNIKVRINHRYDIVN"
     gene            31827..32510
                     /locus_tag="KR250_002405"
     CDS             31827..32510
                     /locus_tag="KR250_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH0068334.1"
                     /translation="MQDVKEKVKQDYLKGMKQKEISAKYDISLNTLKSWIKRYNWANE
                     KKKGAPKNKRGAPIGNKNATGPPGNKNAEKFGFFSKYLPEETQDLINEIKDKDKFDIL
                     WEQITIQYAAIIRAQKIMYVKDKEEMIKELKKYESTENGEKIEYEFQFAWDRQASFLN
                     AQSRAMSELRSLIKQYDEMIHKDWNLATEEQKTRVEKLKCEVDNLSKDDIGDDELKIS
                     VDYGDRNDS"
BASE COUNT        13270 a         3421 c         5559 g        10290 t
ORIGIN      
        1 taaaaaaata aaatattagt tttgatttct ggtttaaatt gggttataat aaattgtcaa
       61 attatttatt aatataaaat agactataaa acaagcaaaa atcatactat tatataaaag
      121 accttaaaga tagaaaacta cagaaagaag ccaaaatgtt tttgaaaaaa atgaaatttt
      181 attgcaaaaa aatcaaaatg aatttaactt aatagttata atctattttt agtgttacaa
      241 atatacaatg tatattatac aatataaaaa atacatttac ttagaagtat tattatattt
      301 ggatttttgg tttttataat ttttttatat ttaaacatag ctaaaaataa aagattacga
      361 aagctaagta gagtttattt tattttaata aataataaag ctatttttct atatattggc
      421 ataatatatg cttatttaac tatgaaatta aaattaaata ggattaatga ggaggaaaaa
      481 aatgggaagt aatgaactga aaaagactct tggcgtatca gcagcattat caactgttgt
      541 tggttccgtt ataggagcgg gcgtattttt taaaccacaa gcagtctata cattaactgg
      601 aggagctcca ggtcttggta tattggcatg gttaatagct ggaatcataa ctataacagc
      661 tggtcttaca gctgcagagg tatcagttgc gataccaaaa actggtggaa tgatggtata
      721 tataaaagaa atttatggag aaaaattagg ttttttaact ggatggatgc aaattgtatt
      781 attttatcca gggatgatgg cagcattagg tgttatattt ggagaacaag catcagcact
      841 aattggtagt ccatcacttt tacttccaat agcaattgga attatagtaa tagttgcagg
      901 gcttaatatg ttaggttcaa aaactggagg agtcatacaa actgtttcta caatttgtaa
      961 gttaatacca cttattttaa taatggttgt tggatttatt aaaggtggtg gaaataatcc
     1021 aatacttaca cctatggttg gtgaaggatt aagcctgggt agtgtactag gtcaagtttt
     1081 aattgctata ttatttgctt ttgatggatg gatgaatgtt ggtactttag ctggagaaat
     1141 gaagaatcct ggaaaagacc ttccaaaagc tataataggt ggattgtcag ttgttatggc
     1201 tgtttatttt ataataaatt tagcgtatct ttgggtatta ccagctaatg aattagcaaa
     1261 ttatgcttct cctgcatcag cagttgctga agttatattt ggttcaatgg gtggaaaaat
     1321 aatatctgta ggtatattaa tttcagtgtt tggagcatta aatggatttt tactaactgg
     1381 ttcaagggtg gcatatactt tagctacaga taagacttta ccaaagtatg gtatattttc
     1441 taaattaaat tctgctcaag ttccagcaaa tgcaatagca ttagtatctg ttattgcatc
     1501 tatatatgct ttgtcaggtc aatttaattt attaactgac ttagctgtat ttgctacttg
     1561 gatattctat gtattaactt ttataggggt tatgaaactt agaaagactc atccaaatat
     1621 accaagagag tataaagttc cattatatcc tattgttcct ataatagcta tagcaagtgg
     1681 tatatttgtt gtagttaatc aattgtgttt tgcaggtatg aaaactacta tgatatctat
     1741 aggaggtctt gtaataacag ctataggctt accagtgtat gcttatatga ctagaggagt
     1801 aaaaagataa accaagtagt ttacaaatgc ttcataatta aaataaaaga gactgtctca
     1861 aatagagata agtctctttt ttattgttaa aatggtaaag atatgtttaa ttttacaaaa
     1921 aaagagcgtt ccatgaacta aaaagtttat tttaaggtat tcattttatt tttagttttt
     1981 tgattataaa attaaacatc taaaaaatta tttattttaa aatactttat tttaaagtgt
     2041 tttttatcaa actaagtggt atctgtacaa caaatattat acatagcttt tatgaaaatt
     2101 aaatctttct gttatatata atagaatatt gtattatata gaaaatatta cctgattaat
     2161 atagtgattt ataaatgaga ttagaaaatt ttatttgata acaaaaatta gataatatga
     2221 atatataatg tatgtattaa aaatgtggca aatagttgtt ttgctattta ttatttgttt
     2281 aatatttcta atttataaaa aaaaaattat aaattttaaa aattataaat gacattttaa
     2341 ctagaattaa ttgtaaaaaa cactatataa tactcaattt tcaacattct atacaattat
     2401 acagaaatta tacagcataa taatcaccga aataacaaat tttaaaaatt aagcaaaact
     2461 ttttgtcgaa gttcaacgtc tatatatatg tagtgtaaat ttttgggata aaatactaca
     2521 aaaatataaa tgtaaaaata tagtaatatt ggaggtcgaa cgatgagaaa taaaaagaaa
     2581 aaaataaata gaaagaagtt gtatttgctt ttaagtgcta ttgtagtgtg tctggctttt
     2641 atagtagttg gtgttcgcgt cagctcttta aatatgaagg aaaatgaaat tagtagaaat
     2701 gcaaaactgt caagttctac tattacagat atagtatcta atacatcaat agaagttagt
     2761 aaagggccaa gtaaaagttc tggaaaaata gcatacataa ctatagatga tggtccatct
     2821 aaatttactg acgagatgat aaaaacttta aataaataca atgttaaagc aacttttttt
     2881 atgatagatg ggaatatgaa ggaatatcca cagcaagtta aaaatataat taaaaatggg
     2941 aacacagcag gttttcatag tgtatcacat gatattcaca agctttatgt tactagtaca
     3001 tcagcaaaag aggaatttga tacaaatgat caaacttttt ataagataac tggtaaacat
     3061 tcaaaagtaa taagaatacc ttatggaagc aaaccatata caccacaagc ctcttatcag
     3121 gctttagttg atgctggata taaaatttgg gactgggact tagatacaga agactggaga
     3181 tctaattctt ctcaaattgt acaaaatgta aaaaatcata ttaagaatag aaaaggtgaa
     3241 gataaagacc aacttgtagt tttaatgcat gagaaaaaac agagtgcaga agctttagat
     3301 tcagttttaa aattcttatc agatgaaggt tatgaatttg cgccagtaga tcaaaaccaa
     3361 ataccaaaaa attattggtt acgtaattta gagtagttaa aatataagaa tattgattga
     3421 tatttttaga aattgagaat aaaaatgagt gaggtgtagg tatattgaat aaaaaacttt
     3481 atataggaat ggtcggaata ttgtctttga tgatggttgg ttgtaataaa agtttagcaa
     3541 aagtaaatga tgtggaaatc acaaaagaac agtacaaaaa aacaaaagca gttttatctg
     3601 ctactaataa ttatataaat gggcagtctt tagatgaatt agaaaaaact ttggataaaa
     3661 aaggtagaaa taaattagaa aatgttataa tatcgtttat ggtggataat gaacttttat
     3721 atcaagaggc taaggataaa ggactaactc caagtaaaag tgaagtagac tcaaaatatc
     3781 aagaactaga agataagatg aatttgaata caagctataa ggaaaaaatg gacaaagcag
     3841 gtgttgataa agattattta aaacaagaaa tatctagaga tttagcaata gataaaaata
     3901 aaaaggcctt tgaagataga ataaacataa gtgataatga tatggaagcc tactatacaa
     3961 gtcataaaaa agattttaat gtagaagaag taagtgcttc tcagatactt atatcgactt
     4021 tagataaaaa taaaaaagaa gtaagcaaag ataaaaaaga agctttgaag aagaaggcag
     4081 ataatatatt gactaaaatc aaaaatggag aaagttttga aagtttggct aaaaaatatt
     4141 cggatgataa agcaactgga aaaaatggag gacagctagg atattttaca aaggatgata
     4201 aaaatgctga gtttacaaag gaagtgttca aattaaagaa aaatgaagtt tcaaatgtgt
     4261 ttgaaactag ttatggatat catatagtca aagttactga taaaagagaa agacaaaaaa
     4321 gttttaatga gtgtcagagt ttaataagag aatctatttt aaatgaaaag tatattgagc
     4381 atataaaaaa actgaatgaa gatgctaaaa tagacagata gaatttctaa cagctcaatc
     4441 ctagcctgta atcggaggga gattttacag gaatgtttaa gagtttaaag agaacgaaag
     4501 ttgagaaata cattatcgat aataaagact ctttttatag aatagcatat agttatacga
     4561 agaatgaaga agatgcctta gatgtggttc aagaagccat gtataaggca ctatactcag
     4621 tggaaaatat taaagaagta aattatataa agacatggtt ttataaaatt ttagttagaa
     4681 cgagtataga ttttattaga aaaaaccgta aatataataa tatgacagat attgacctta
     4741 tagatgagac tggagagtat gataaatata ctgatttgga tttaagaaga gctttggaag
     4801 aattacctat tgaatacaaa tctattataa tattaagatt ttttgaagat ttaaagattg
     4861 aagaagtagc aataatattg gatgaaaatg tgaatactgt taaaactagg ttatatactg
     4921 ctttaaaaaa actaaaatta aaaattgaag agtagggata aaaagaatga caagcaaaga
     4981 aaggttaaat aagttaaaag atgagtatca taaaattcca atacccaaga aattagatac
     5041 tataataaat catgaaaaaa tagaaaacat atatcgagag aaaaataaaa aagtaaatag
     5101 attaagattt aaagttgcaa tagcctttgc atgtatattt acagtattag taaatataag
     5161 tcctgtattt gcagacaatt tttctaagat acctgtaatt ggagcaatag tagaagtaat
     5221 aactataaaa aattatagtt taaaaagtga aaattatgaa gctgagattg atattcctaa
     5281 aatacgaggt ttaaaagata aaaacttaga acaaagatta aacagtagtt ttatggaaga
     5341 tggaaaaagg ctttatcatc aattccaaga gagaatggag aagatacaat catcaaagaa
     5401 taaaggatat aaatctttaa gtttgagtta tagtgtaaaa aataattcta aaaaatttct
     5461 atcaatagag atgacaaaaa atgagataga ggcttcatca tatgtgagta aggtacatta
     5521 tactattgat aaaaaaagac aaatagtttt gactttgcca atgcttttta aagatgataa
     5581 gtatataaaa gttataagtg ataatataaa agaacaaatg agagaacaaa tgaaaaagga
     5641 tagtacaaaa tcctatttta tagatcaaaa aaaagacttg cctgttgaag attttaaaac
     5701 aattaataag tatcaggatt tttattttaa taaaaatgaa gaccttgtaa tttgttttga
     5761 tgaatatgaa gtagcacctg gttatatggg cgcagttgag tttgtaatac catataaagt
     5821 aataaaagat ttgtaaattg aatagtagtt tatatataaa tcttttaagg aggacaatat
     5881 gactacatat aaaaaactta aaaaatctat atttaaaatt gctatatttt caatatgctt
     5941 attagtttca ttagtagata caaaccctat atttgcaggt agttccttta atgaaacttc
     6001 aaatagtgat acgataaagg tgttaaatgc taagagtatc aactttaaac aagacaatta
     6061 tgaagtgaat gtgagtattc ctaaaataaa aggtttaaaa gataaaactt tagagaataa
     6121 attaaacaat gagtttttgc aagatgggaa aaaaatttac agtgaatttc aaaataaaat
     6181 gaaaaaggaa aataataagg gtcatagata cctaaatgta atccataatg tcaaaaataa
     6241 ttctaaggac tttttatcaa tagaaatgac aaaagaagag atagaagctt cctcatctat
     6301 aactaagaag cattacactg ttgataaaaa tagaggaata ctattaaatt taccaatatt
     6361 ttttaaagat agtagatata ttagagctat aagtgataat ataaaagagc agatgagaaa
     6421 tcaaattaag aaagatgata caaaatctta ttttatagac caagataaag actcgccagt
     6481 gaaagatttt gaatctataa ataagtatca agatttttat tttaataaaa atggaaatct
     6541 tgtgattagt tttgatgaat atgaagtagc tcctggttat atgggagcag tagaatttgt
     6601 gataccaaat aaagtaataa aaagactaag atagatatat agaagatttt tagatagact
     6661 ggtaaggaga attatatgaa tatatatgaa caagcaataa gattaataga aaataatgag
     6721 gatttcgcat ttgctacaat aacgtctcat tcaggttcaa caccacgtga aactggagca
     6781 atgatgattg tgaaaaatga ttctactata tttggctcag taggaggagg tagtgtagaa
     6841 gcagaatgta taaaacatgc aattaatgtt ataaaaaaca gagaatcaat gttgtataaa
     6901 tttactctaa acaaatctga tgtggcaaaa ctaggaatga tatgtggagg tactggagaa
     6961 atacaaatag attttataga tagtaaatta aaaagtaata tagaaaaatt taataagaga
     7021 ctaaaggaaa atacaagtaa agcatatata tttggtgcag gtcatatttc aagagatgtt
     7081 gctgtgatac tatctctttt agaatttaga acagtagtaa tagatgatag agaagaattt
     7141 gctaatcatg aaaggtttcc agaatcagaa gtagtagttt tagattcttt tgaaaacata
     7201 ccagattttc ccactgacga aaatagttat ataattattt taacaagagg tcatctatat
     7261 gattcaagtg ctttagaatg ggctcttaaa agagaggctg gttatatagg tatgattgga
     7321 agtagaacaa aaattggatt gacttatgaa aaactcatga aaaaaggttt taagaaggaa
     7381 gaattatcaa aagtacatgc accaataggc ataaaattga atgctcaaac accagctgaa
     7441 atagctgtat gtatagctgc tgaacttata aattgtagag ctaataagga aaaataaact
     7501 aatgaacaca gggagtaaag atattgatac tttaaaggtt aattttagag ggattgaaat
     7561 aaaagcagtt ttagtatata aaaatagaaa aaatatttct ataaaaatag atccatttgg
     7621 caaaataact gtaatgagtc ctcctaaaat atccaaaaaa ataataaaag atataattat
     7681 tgaaaaagga gactggatac ttaaaaagtc agaaaaatgt aaaggaagag aggaagtata
     7741 taaacaaaga atgtttataa ctggcgaaaa atttctttat ttaggtgaag agtaccatct
     7801 agttataaaa gaactgttga gagattctgt caaaaaaagt aactgtagaa taactattaa
     7861 cgaatatcaa atagttgttc agacaaatga tacaagtaca gattttatta aaaaatcttt
     7921 aaaatcttgg tataagatgg aaagtgaaag aatagtatta gagagaatag attttcttaa
     7981 attaaattgt gaaataatga agcaattagt tccagcgagt gttaaggtaa aagaacaaaa
     8041 taagagatgg ggaagttgta cagctcaaaa aaatatttat ataaactcaa aaatatctat
     8101 ggcaagatta gatgtaattg attatgtaat aatacatgag tttagccatt tagcacatat
     8161 gaatcactct aaaaaattct atgatttagt aaaatccatt atgcccgatt ataaagataa
     8221 agaaaattgg ctaaaaataa atggatataa aataataatc tgaaaacgat tattccaaaa
     8281 tataggcgta aagtataaaa gaagtattat tgtgaaaaaa ataaataatc tgaaaatttt
     8341 aaaaaaagtc attgacattg taaatgatag agaatataat ctatattaac aacaaaacag
     8401 aattgaaata taaaattaaa aaattaaaag caatgacaga gacaacaata ctaatagtgt
     8461 tatccacaga gaaccaatgt tgctgagaat tggtgttaac agtagtataa tgatcactct
     8521 ggagcttttg tactgaatat ttttagtaag tacaaacgta tgtcaacgtt acatgactaa
     8581 ggttaaggta attaattacc cgacctgaaa aaagaggggt agatgtattt tttatatcta
     8641 caaataaggt ggtaccgcgg aatataactt tcgtccttat aagtaatatt tatattgctt
     8701 ataaagatga aagttttttt attttaagtg atataaataa atttaatcca tataaaatta
     8761 ataaaaaaat aataaaaata aggagggatt tttatggaaa atactgtata tgctaaatta
     8821 aaccaaggaa tagattcttt tttaagagtg gctatgactc taagaagaag agaggttgat
     8881 atacaatcaa taagtatgac tgtagataat atgaataatt caggtataaa attaatagta
     8941 aatgaagaaa agacatcctt agattctgtc ttaaaccata tgaaaaaact acatgatata
     9001 agagagataa ctgttgaaag aatgagacat taattaaata tattttataa aactaaagat
     9061 aaatatgctt attttaaaat aaaaaactaa agataaatat taaataagaa aaatcttaga
     9121 ttaaaatata agtgtcattg aatttattag tttatcacag ttaataaatt tattagtttg
     9181 ttgtagctaa taaaaactga aaattctaaa attataattg taagattata taaatttaga
     9241 atttaaatct gttgaataat attaaaacaa tgataatgag tatgaatttc taataaaaat
     9301 aaatatcata aaaataagaa aaagtattgg gaggaaatga aaatggcaag aatgtattat
     9361 gaaaaagatg tggatttaga ggttttaaaa aataaaaagg ttgcagtttt aggttatgga
     9421 agtcaaggac atgcacatgc acaaaatctt agagataacg gtgtacatgt agtgataggt
     9481 ctttatgacg gaagtaaatc agcacaaaaa gcaaaagaag atggatttga agttaagagt
     9541 gtagcagaag ctacaaaaga aagtgatttg acaatgatgt taatgcctga tgaaaagcaa
     9601 aagaaagttt atgaagagag tgtaaaggat aacttaaaag aaggtcagac attagctttt
     9661 gctcatggat tcaatataca ttataatcaa gtacaaccac cagaatttgt tgatgttgta
     9721 atggtagctc ctaaaggacc tggtcactta gttagaaatg tatttacaaa gggaagtgga
     9781 gttcctgcac tatttgcagt atatcaagac tacactaaaa aagctacaga gacagtttta
     9841 gcatatgcaa aagggatagg agcaacaaga gcaggtgttt tagaaactac ttttaaagaa
     9901 gaaacagaaa cagatttatt tggagaacaa tcagtacttt gtggtggaat aagtgaactt
     9961 ataaaattgg gatataaaac gcttgtagat gcaggttatc aaaaagaagt agcatatttt
    10021 gaatgtttac atgaaatgaa actaatagtc gaccttatat atgaaggtgg atttgaaaga
    10081 atgagatata gcataagtga cacagcagaa tatggagatt atgtatctgg taaaagggta
    10141 ataactgatg ctgcaaaaca aggtatgcaa aatgttttag aggatataca aaatggaaaa
    10201 tttgcaaaag cgtggattaa agaaaatgaa gaaggacgtg aaaacttcct aaaaactaga
    10261 gaagaagaat ataatacaga aattgccgaa gttggtagaa atctaagaag tatgatgtca
    10321 ttcttaaaat aaatatatat taaatggatt agtttgaggg aaagagtggt gtatagtagt
    10381 gcgtatgagt ggagcaaagg taattttaga atgtttaaag aaagagggaa ttgacacaat
    10441 atttggatat cctggtggag ccgtgatacc tctttatgat gctctatacg attattcaga
    10501 tgattttaaa catataagga catcccatga acaaggttta gttcatgctg cagatgggta
    10561 cgcaagaagt actaatacag taggagtttg ttttacaaca tctggtccag gagcaacaaa
    10621 tgccataact ggaatagcaa cagcttttat ggattcttca cctatggttg taatttcagg
    10681 gcaggtgcca actagtttat taggaaaaga ttcttttcaa gaaattgaca taactggagc
    10741 aacattatct atgacaaaac ataattattt agttagaaac actaaagaat tagttcctac
    10801 aataaaagaa gcttttagag ttgcaaattc aggtagaaaa ggacctgtac ttgtagatgt
    10861 tccaaaagat ttatttttag cagaaatgga ttttagtggt gaggactatg atttatgtca
    10921 aatagatgat tatatggatt ataagagtga ttttgatcta gatgatgaaa ctaatattaa
    10981 gcttttaaat gaagcaatag atatcataaa agaatctaaa aagcctataa tatatgcagg
    11041 tggaggtgtt aaatcttcag acagtgagga aattcttgaa aaatttgcaa ctaagataga
    11101 tacacccgta ctaaatacac ttatggggct tggaaatata gatagaaaga atgaattatc
    11161 tcttggaatg gttggaatgc atggaagtag agaaagtaat ttagcacttt ctaattcaga
    11221 tttagtaata gcgataggtg cgagatttag tgatagagtg ataagtaaaa gttctgaatt
    11281 tgctaaaaat gcaaaaataa tacacataga catagaccca tctgaaataa gtaaaaatat
    11341 agaatctaat gtatctttgg ttggagatgt aaagctagtc cttagtttat taattgaaag
    11401 ggttgaaagt aagaataata gcaattggaa agaagagata aagagattta gaaagagtga
    11461 gggtgtacaa acatatgaat ttcatccaca aaatatacta aaaaaaataa atgaaaaata
    11521 tgaaacatta aagaaaccca ctgtggtagt aactgatgtc ggtcaacatc aaatgtgggc
    11581 tgctaagtac tggaatttta aaggtaataa aagttttata acatcagcag ggttaggaac
    11641 tatgggattt ggtttaggtg cagctattgg aactaaagtt ggtaatgtag ataaaaatgt
    11701 agttttagtc actggtgatg gaagttttag aatgaactgt aatgaactag ctacagttgc
    11761 aaattataat gtacctatgc ttattttatt actcaataat agaacattag gaatggtaag
    11821 gcaatggcaa aagttatttt ccaatcaaag atactctcaa actgacatta atgaaaatgt
    11881 ggattatgtt aaactcgtaa atgcatataa tattgatgga tataaggtat ccagtatgga
    11941 agaattagga aaggcgctag atatgataga ttttaataaa cctgtatttt tgcaatgtga
    12001 tatagataag gattatgatg tttatcctat tgtagcacct aatgatgcat tggaaaattt
    12061 gatatgtaac taaaacttat actgaatatt gataaaattt tttattaaat ttttattggt
    12121 attcagtttt tgttgtttag agatagattg gcatttttta tttttgcaaa taaatcaatg
    12181 actatataat taagaactta ttaaatttta attatacaat ttagaatagt attacaactt
    12241 aagtaaatat taagttttat ttgttagatt tattattaat accttatatt agttatggtt
    12301 gttatatact ctaaagcctt gaaattacaa gtatatagat gattatataa atttgataat
    12361 tttagctttt aaaatagata aattagattt tgccacccat ttgccaccgt attatatttt
    12421 tgggtggcaa actctaattt gtaatttttt caaatatatc tacagtttca tttttcattt
    12481 tatcagttac atgtgaatag gtatccattg tagttgatag ttggctatgg cccaaacggt
    12541 tttgtatgtc tttaatgtta gcaccatttt ctaataatag agtagcatgt gcatgtctta
    12601 aagaatgaaa atggaagtca ttgtttaaag ctactcgaat ttgtcttact atagtgtcta
    12661 aagtgtgagt attcacttgt tgaccatttt ctttggtaca tacccagtca ctatcaaagt
    12721 aaaattctcc atattttaat ttcattttct tttgatataa tttatgttct tttaatgcct
    12781 ttattaaagt gtcacctgta aatatagttc tgcaagagct ttctgttttt ggttgcccta
    12841 attcaaacat tccatttggt tttttaatca aagtatgtct tactgtgata gttttattat
    12901 caaggtctat attatcccat tttagtgcaa taatttcacc tcttcgcata ccagtatgaa
    12961 atccaattag taaaactata cgttgaaatg aatcttgagg aaatatattt agtatttgat
    13021 taaattcttc taatgtaata gttttaattt tattagtttc tgttttagat ttagtttttg
    13081 gtatgctcac atattgcata gggttttctc gtatatgttt gtaaggatgg actgctgatt
    13141 ttaatgacct atgtaatatg gcttttaata cttgtaatgt attttgagag taatcctctt
    13201 tgtactttct gtttatgaag ttttgtagta ttgcaggagt taaagctttt actttgtaag
    13261 ctcctagttt tggctttata tgtttttcta tgtttattcg gtagctttct tgagtgttgt
    13321 atttacagtt aagtaagaca tattctttgt accaaaagtc taagtagtct gataaactga
    13381 tattgctttc ttcaaatact atgccagagt tttcatattc atttagtgct tctcttaagg
    13441 ctttttctgc ttctttttta gtattgcctc caactctttc tacttttttt ctttttcctt
    13501 ctactatgcc taggtcaaag tagtaatacc atttgttact tctttttctt actccacctt
    13561 tcataatagt atccctccct tttagaatgt atgtttgttt ggtgtttata taaaagagca
    13621 gattaactgc tctttatata cttttgttat gtacaatatt atcttaattt tgtttatttg
    13681 gtttttactt aattattttc ttaatattct tacaattttt tagcttttat gtgtagttta
    13741 ttataataat attgaaatat atatttatta agtaaaaaat ctataaaagt agaataaatg
    13801 agtttaaaaa atatcctcat atatgtagaa atttggcaaa ttagttaata ctaaaaatct
    13861 attatttcct aagtccaaca tgcttttctt ttttgataga aattctaatc tttttagtaa
    13921 gaagttgatg ctaacttgta attcttcagc tatttcgtat acacttgtag cgtgtgaatt
    13981 aataacatgt attatttctt cttctgttat aagaaattca catgcccatt ttagtgcttt
    14041 gttttcagtt ttgtctatca agattttatt tttgtaactg ttttttgaag atacatagtt
    14101 tccaacactg gtaaaatgat gtcctaattc ttctgctaag atttctatta gtttagcatt
    14161 gttttgtttt aatgaattaa gtaatgatat aatttttagt ccttgtctgt ttatatacaa
    14221 tccttttatg tcatctgcta ttttgtcagt gtagtaaatt tctatctctt cattatttgc
    14281 taagtctaaa agtgcgtcta gtttgttcat tgaaatcccc ctataaaaag aatgtatttt
    14341 tgatttttgt tatatataaa gagcaggtgt gaactgctct aaatattcat tttttatcca
    14401 aaaaagattg gataatttta gctgtagaca aaatatggat tatagtatat gattataata
    14461 taattacata taatatatgt aagcacttac aaatgtgtgt tagtatagtt agcactaaat
    14521 ttgtatttgt gcttaaagcg attaaaggag ttttggagcg gtgggtagag ttttactcac
    14581 tataaccaag actcttttgc atattagagt atttcatgta gtcatatatt ctaataagcc
    14641 ttcttaaatt tgttttttcg cttgtttttg cagactctaa aatattagga ttttcctcaa
    14701 atattttctt aagtagattt tctaactgtt cagtgtcaat ttctttttct tctaaaaaag
    14761 caataagtct tgtagctttt tcaaatgatt gatgttcatc attccaaata ccataaacac
    14821 ttctattttt tatgtaagta ttatttctat tttttttaat actattgcta ataatatttt
    14881 caaagtcact ttctagtgca attgtcctat aatctggaaa ttcaagtgtg ctattaaaaa
    14941 gatgtttatt taatggtaat ttattatatt gcttaattaa agatggataa gcatattcaa
    15001 gtaattcatc tgatgaaaat tctaagtcat tcatgactat atcacgatac cattcatcaa
    15061 aacttaatcc ttttaatcct ttaagaccaa aatcacttgc cttgccaact gctaaaacta
    15121 aatcttcatc ttttattctt gtatcatcaa cactaccaac ccgaatttta gcagtaggtt
    15181 tgttagtaat tgtgaaatcg taaaattctt gtttgaacat tcttaatatt ctaactggta
    15241 attttgattt tttttcagat aatgcctcat agagtaaatt aaaatcctcc agtttaattt
    15301 tagtcatagt aatcatttta ttatcaaaaa caattgtatg acttgatatt tctatatcat
    15361 taaagttttt ttcatactca atgaatatga aacgttcctc gagtttttta gcattttcat
    15421 tagatagaca attaactata gcattaagta ttttttttat atttacatct gtaacagagt
    15481 aaccaataaa tatgatagga aactcaacaa atatagtcat tagcttagct gccaagtagg
    15541 cacttttgct gtcaaagtct atataatcag cttcatttat tacaatacta tttggagaag
    15601 aaacacatcc atgaatttta taaatttctg caataccttg tatagatgaa aaaactaaat
    15661 tttcttgacc tatatatgtt gtataattat caacgatagt ttccaaaaaa caatcataat
    15721 ttgttgtaat aaatcctgat aaactttttt tagagatatt actaagtttt tcaacttcat
    15781 cttgatattt atctactatt ttagaattat ttttaatata catagcaatt tctgccttaa
    15841 atggagaaac tcctgattta acaatatcta aatacttaga gtctaagctt cgtatagaag
    15901 aatctgaaaa ccatttttta ttaaaatcat tttcaataag ttctgcaatt ctaggattta
    15961 tacccatttt tgggttctct gattttgctt tatttttata acttgtatat ataaagtcat
    16021 cattactaat tttttctgca aagactttta gtaaatcctc ccaatttgga agattatagt
    16081 atcttctagt taaaccagaa ccaacaaata aaaatggtgt agcattaaat cggtttatga
    16141 catctgatat tatagatttc atataattta tcacctactt ttaatttatt ttttatactt
    16201 atttattaaa aattcaatat aatcattgag ttgttcttga gcttcctctg gtaaatcttt
    16261 gtgaggatta actctatgtg cagctacagt gtctatatca ttttttacaa tagacctacc
    16321 taataaataa tcagtagtca cattaaatat ttcagcaagg ctagatagca tataagcatc
    16381 aggaactgtc tttccttgtt cataataacc ataagcgctt ttggttaagt ttaatttatt
    16441 tgcaacatct tcttgagtca tatttttttc agctctaagt tcttttagtc ttttttgaaa
    16501 taatgtatta ttacaagaag ttttgctaga aatattgctt gctttattat catacctacc
    16561 tattaaataa tcaacagtag tgccaaaata ttcagctaaa ctagataaca tttctggggt
    16621 aggtgttctt ttaccttgtt cataaaatcc atatgcacta gtagttatgt taagatagtt
    16681 ggcaatttct ttttgcatta cacctttttc tttcctaaga aatttcaatc tatctgataa
    16741 aatgctcata taatcactcc tcttaataaa gttgccttta ttaaataata caacaaatag
    16801 ttgtaaaatg aaataaaaaa ataaaaaaac tatttacaaa caacatatag ttgtgtatta
    16861 tataagtata aacaacaaaa tgttggtaag gaggatatag cttgaacaat ctaaaggaac
    16921 ttaggacaga aaggaattta aagcaaaaag atgttgcatt aaaagtaggt ataactacta
    16981 gttattatgg aatgattgaa agtggaacaa gagtaccaag tttagcaata gcaataaaat
    17041 tatctaaatg ttttggagta tcaatagaaa aaattttttt aaatacaaat acaacaagat
    17101 gttgtttaaa agagaggagt taaatatgaa agatttaaaa atagtaaaag ttaataataa
    17161 actaacaact gacagtagag atatagctct aatggttgaa aaagaacaca agattttact
    17221 aagggatata agaaattata taaaccaaat ggaagaagcc aataaaaaca tgagtacaga
    17281 tttgtaccca tctgattatt ttattgaaaa tacttattta gatgattata aaagagagaa
    17341 gccatgctat gcaataacaa agataggttg tgacttcata gcaaataaaa tgacaggcat
    17401 aaaaggtacg gcatttacag gaatatatac aaaaaaattt gaagaaatgg agcaagtatt
    17461 aaaaaatgaa caacctaaac taccaactac atataaagaa gcattacaac atcttataga
    17521 gcaagtagaa gtaaatgaga aattacaact agaaggaaaa atgaaagacc aagtaataaa
    17581 agaactaaaa ccaaaggcag attatacaga tatgatactt aaaaacaaag gacttgtcac
    17641 aataactcaa atagcaaaag actatggaat gagtggaaaa gaaatgaata aaatacttca
    17701 tgaaagaggg attcaatata aacaaagtgg acaatggctt ttatataaac aacaccaagg
    17761 gaagggatac actcattcag aaacaataga cataactaga agtgatggaa tgactgatgt
    17821 aaaaatgaca actaagtgga ctcaaaaggg aagattgttt ttatatgact tattaaaagt
    17881 aaataacata ttaccagata tagaaaaaga gtatagttat caaacttcaa tgttatgtta
    17941 gtactttgaa aaataaatac agaatattca aaaagaggtg atttaaaggt tgagtaatag
    18001 aaagaagtat accttgagta ttacgaaaga attatacaat aaatgtaaag aaaaagctaa
    18061 tcaaagaggt atgtcagtga atgattacat actatcagtg attagcaaga gtatataaat
    18121 tattcttcaa tattaatttt aatttctcct tttctttgct cataatcttc aatatgtttt
    18181 tttattattt gttcaatttc cttattagca gaacgacctt cgctttcagc tatatattta
    18241 attttttcaa gcagaactct gtttattctt aaagtgtatt taggtagtga agttgacata
    18301 ataatacctc ctttttaaag tctaaatgat gtcataatta tatcataaaa ataatttata
    18361 gaaaatctat aaaatatatt gacggcataa agacggcgtg atattattaa aacaagaaag
    18421 gggttgacgg caaaatgaac tcaaataaaa agagaataac tgttaggatg ccagaaaaat
    18481 taaatgaaga aataacgaaa aaatcaaaat acttagggtt aactaagaac tcatttatat
    18541 tagatatact atggaaggaa tttgagttat tagaatatag aagttataaa aaggaggtag
    18601 ataaacatga ataacttaca agtaatagaa agaaataatg agagagttct aactacacaa
    18661 caattagcag atgtatatga aactgactca agaaatataa gtaacaattt caacaataac
    18721 aaagataagt ttattgaagg taaacattat tttttattgc aaggtgatga tttaaagaat
    18781 tttaaaggca ttcatacaga atatgaaaac ctaaaatttg cttcaaaaat gtatctttgg
    18841 actgaaagag gagcaaatag acactgcaaa atattagata cagataaggc ttgggaacag
    18901 tttgataatt tagaggaaac atatttcaaa gttaagcaac ataagccaac ttgtatagaa
    18961 gatgtattaa tagaaagttt aaaagaaatg aaagatttaa gacttcaagt taatcaagca
    19021 aatagcattg ctttagaagc aaagacagag gttgaaacaa taaaagatgt agtttcatta
    19081 gactcaaata gttggagaac aaatacacat caactaattg caagaatagc aaaaaaacaa
    19141 ggtggttttg aacatataaa tatgcttaga acagaaagtt atgaattatt aaataagaga
    19201 tttggagttg acctacatag aagattgatc aataaaagaa gaaaaatggc agaagaaggt
    19261 gtatctgaat ctaaaagaga gaaagttaac aatttagatg taatacaaga tgataagaaa
    19321 ctcatagaag ggtatgttgc tattgtaaaa gatatggctt taaaatatgg aatatcaagt
    19381 gatttaagca aaaattaagt taaatcacaa cagcactttg aaaactaaat ataaaataaa
    19441 aatatctccc ttaaaagttt tttaaagaga gataaacact ttattttgta actatttgaa
    19501 tacatgatgg atgaatgcaa tagtttttat ctttgtaatt tatagaaaca taattactag
    19561 agtacatttt atcaaggttt tcattaatat attcaattgc attacagcca tttatagagc
    19621 caatatcagt tatatcaatc cagttattaa atatcttaac ataaacaaac ttcatgataa
    19681 caccaacttt cattagaatt taagttaatt ataacatggt gtactgagat aatgtgttaa
    19741 gaaatagata cagaatattc aaaagaggtg attagatgga aatagagcaa acaacaatac
    19801 gcctgctaaa aagcagacga aagttgaata ttaatttcta ttttggttag gcgaaagacc
    19861 taacaaaaaa tctgtagata cattaagttt gttagaaatt aatattaaag tttctagatt
    19921 tggttctctt gaaccagctt cataatactg atatgcacgt tctgagatac caaacaaact
    19981 agcaaattgc ttttgagtca tattcatttg ctttcttact agcttaatat tatctttgaa
    20041 tttagtcata actacacgct ccaataaaaa aataaataaa tttcttgaca cgaacaatat
    20101 tagcgtgtaa tatataaaac atagcacgaa caatattggc gtgttaaata aaaaggagag
    20161 ggtgaaaata aataataatt taaaactaca acgtgaaaaa attggcttaa cacaattaga
    20221 ggttgctcaa aaagccaaaa taacagagag aagctatcaa tattatgaag ctggcgaacg
    20281 tctcccaaac attcgtacag ctttaaagat agctataatt ttaaatacta attgtgaaaa
    20341 actctttaat gaataatagc ataaaaggac tcagattaca agaaaggagc tttagatatg
    20401 aataatttgc aacttaacaa taaaaataca ataacaacat tagaaattgc tgatatgtta
    20461 gaaatcagac actgggaagt cttaaggaaa ttagaaggaa cagaaaaaac aaaaggaatt
    20521 attgatattc ttaacgacaa caattttgtt gtggttgatt actttataaa atcaacatat
    20581 ttggattcaa aaaatgaaag tagaccttgc tataatgtaa caaaattagg ttgtgacttt
    20641 ctagcaaata aatttactgg agaaaaaggt attattttta cagctagata tgtaagaaga
    20701 tttaatgaaa tggaacgagt attaaaagaa caacaaccta aactaccaac tacatataaa
    20761 gaagcattgc aacagttatt agtagaagtt gaagaaaaag aacaattaca attagaaaat
    20821 caagaaaaag ataaggtaat tcagttacag caaccaaaag tactgtttgc tgatgcagtt
    20881 tcagcttcaa acaattctat attagttgga gaacttgcaa aacttataaa acaaaatgga
    20941 gtagatatag gacaaaacag attatttacc tggatgcgag aaaatggata tttaattaag
    21001 cgtaaaggtg aagattataa tattccaaca cagaaaagta tgaatctaag aataatggaa
    21061 gttaaaaaaa gagtaattaa taatcctgat ggaagtacta aagtaactag aacagtaaaa
    21121 ataactggaa aaggtcaagt ttattttgtt aataagttta aaagtagtaa acaattatca
    21181 atgttaagtt aaatcataac agtaccttga aaactaaata cagaatattt tggaaaggag
    21241 agattgaatt ggaaaataag aaaatagaag aaatagagaa aagaataacc atgcttgaaa
    21301 atcaattgca agcacagtta tttagtcaga taacagctca atcaaatagt gattttatag
    21361 attatatgta ccaatgtata gttttgtatt tacatgagat gaataaaagt gaaaaaagaa
    21421 aaattagtga aattactaaa gaaagaatca gaaaataaga atgataatta caatataaaa
    21481 cctattcttc tagcaggttt tgaagggtca gttttcttta ctgaagttat cataaagttt
    21541 atctgtgatg tgtgttgaag aatttcagaa taaactccat ttactatgcc gtagtagtga
    21601 attagacaag ggttatgata acctacttct tcaacaatca ttaatacatc ttttccaaag
    21661 ttagtcaatt ttagtgcaat ttcatgttca tcatcaagtt ctttttcaaa ttcctctata
    21721 gatttaagaa taattttata ttgaaattca gcatagtaaa aatactctgt gttagaagta
    21781 tcagagactg tctttcttat actttcttgt aattctttag atatattgtt tgaaaactgc
    21841 ataatacacc atccttttga aaattttgga atttattcca tgtttatatt ataccatgta
    21901 gaactgaggt gaatacaatg ttaataggcg acaatataaa gcaaatacta agaaaaagag
    21961 atataaaacc ttataaatta gcaaaggaat taaatataga tgtaagtggt ctatataaat
    22021 tgttgaagaa taaaaattct aatccaacta tagatactct aataaaatta gctgattatt
    22081 tagatattac attagacgaa ttagttggaa gataaatatt tgaaaagagg ggagagaatt
    22141 ttaacatgga tatttctgaa agtataacaa agcaatttag tgatagttta aaaagtttaa
    22201 tagaaataga gataaataaa caagaaacag atagagttaa gagtcaaact gttgaacaaa
    22261 aggtaaaagt actggagcct aaagatatag ttgttttaat aaaaagaggt tatccaaatt
    22321 atttgataac agtagaagaa gcaaggggaa ttttaaaatt agatacagtt tttatgcgta
    22381 ggttagtgag cacaggtttg ataaaatcac tggctagagg tgatggtaga aaaatttcaa
    22441 gatatgaagt tgatgatttt attgaaagaa atcaaggtaa aaatttggat gaacttttaa
    22501 aagtagcaga gaggggggat gaaattgtta agccttaata ctaataagaa taatatagta
    22561 actcttaaaa aagatggaaa agctatagca gacatagtat ttaaagatat taaaactggt
    22621 aagaaaatat cggttggagt attaaataaa aaagtgctgg tcaaataacc aacacacaaa
    22681 aaaataaaaa ataaaatata acacaaacaa attatagcac aaaaagaatg gatttagaat
    22741 aggtaatata acattctaaa aggtttaatg tgtaataatt gtaacaattt agaatgtagc
    22801 ttatatagat agtaataaat tgggagggat ttaaatggaa gcagctagac taatagcaat
    22861 aggtcaaatt aaacaagcag aaaaagaaat atgtaaatta caaggtacaa aaaataatag
    22921 tagtttaatg tggtgggaag ccgtaaaatt tgctagtcaa aatatattag agggtcttga
    22981 acatgacatt gagttagaag cttctattga gtttagagaa gctatgatgt atcaagaaga
    23041 acttgaaaaa gatagaccaa tagatgtcca gatataaaaa aagagccttc gcacaggctc
    23101 taagtgaaaa taagttacaa aaattatagg tatattataa cataaggggg aataaatgaa
    23161 aacaaaaaat gaaataatta agggtttaga agatagatta tttttattaa gatttacaac
    23221 agtagatgaa gtagattggg atgtaaaatt tggacaaata tcagcattag aattttgtat
    23281 agataaacat agaaaaggat gcactttgga acaattcaaa gaacatttag acgaatacaa
    23341 attacaaggg aactatggtg attatataga tggttttgtg tcagttttag aaagaaatat
    23401 tagagaaatg gagggagaaa ttgatggaag tgaataatat ttacataaaa ttgatggatg
    23461 taagaattaa gtttaacaaa ttagatataa aaaagagtgg tcaaaacaag tttgctaact
    23521 ttaaatattt tgagttagca gacttcttac ctcaagcaac agagctgtta caagaagcta
    23581 aattatgccc tatagtgacc tttacaaatg attatgcaac tctaacattg attaatggag
    23641 agaaaccaac agaagagata atatttactt ctccaatgag agaattacaa ttaaaaggct
    23701 ctaatgagtt acaagcatta ggaggaattg aaacatatca gacaaggtat ttgtacattc
    23761 agttattaaa cattacagaa aatgatactt ttgatgctac tagtggaaaa gaagattata
    23821 aaagaaatga cttaacaaac tcctctatta aagcaagtga aaatggtcaa ataaaaataa
    23881 gtcaaaatca aataaaaaga ttgttttcaa taggaaatac aataggcaaa gattcagata
    23941 gagtaaaaag tgaagtgtat tataagttta ataaagaagt taaagattta agtaaacagg
    24001 aatatgacca gatatgtgtt ggatatgaga aattacaaag ggaaaaagga ataattaagt
    24061 aggtgaattt cttgaaggac agagacaaag caactataga gaaaggaaat atacttagtg
    24121 atgggtatgg tctttcacca caattggtag ctagagattc atggctaaca acaggagcaa
    24181 gagctttata tttctatcta tccagttttg caggagcaag tgggacatgt tatccatcta
    24241 gggatattat gactcatgaa cttggtataa ataaagatac ttttagtaaa tacctaaatg
    24301 aactaaagac gagtggttat ataaaagtat ataaaaataa aactagggaa ggaaggatgc
    24361 aaaataatat atatgaggta gtatttgata gaagctatat agaaagtcat atatccatta
    24421 gatgtaagaa agaaaataaa aaaaaccatg tccgaaaaaa gcagacatgg aaccatgtcc
    24481 gaatttaccg gacatggttc aaccggacat ggaaaagtcg gacactataa gtaacagtat
    24541 tataaataac agttttaaaa gtagtatgta tatagagcaa gctgtggata actctttaaa
    24601 agaatttaag aagctatatg aagaaaatat aggagtagta tatccagtca cagctgaatg
    24661 gttattagaa gtatctaatg aagtagatat aagagtattt aaaagagcta tagagatatg
    24721 tgctgaaaga atgaatatga atttatcata cttaaaagga atccttaaaa agtggaagga
    24781 tgcaaatata actacatatg aacagttaga atcatataaa ttacaacatg aaaataaaaa
    24841 gtcaaaaaaa accaatagtg tagtaagcaa aaataagttt gctaattttg aacaaacatt
    24901 tactcaatac agtaacaaag aattagatga aattataaag aaaagccaaa aggctaaatt
    24961 taaataatat tgatggaggt attaaaatga atcaagttgt attagttgga agattaacta
    25021 gagacccaga actcaaatac ataccaggaa caggtacagc agtagcatca tttacaatag
    25081 ctgtagacag aaattatata aataaagaag gaaaaaggga tactgatttt ataccaatag
    25141 aagtaatagg taaatcagct gaatactgtg caaattacat aacaaaaggg aagctagtag
    25201 cattagaagg gaatataaga gttgacaatt atcaaactca atcaggtgaa aaaagaatat
    25261 ttacaaaagt cagtacaaaa tcagtacaat cattagaaag caagaataaa tcgagtaatt
    25321 catataaaga gagtgtacaa gatggaacca taggactaga ccctcaagga tttgaaatta
    25381 tagatgatga tgagttacca ttttaatctg aaaaattaaa tatgaggtga aataaatgtt
    25441 taaagtagaa aggtatttta gtggctcagt agtggacaac cttattgaag atgaccttac
    25501 atgtagaaac tacttagcat tatattgttg tttgttggga attacaaaaa atggaaaaaa
    25561 gatatatcct aagccagaaa aaatgttagc tgagtttgga gtaaagaagg acagaaaaat
    25621 aaaaaaagag ttaccagtaa gaattagaaa tgttaataca ggggaagtaa aagaatttga
    25681 gtctatagat ggtgcagcct gttttttaag attaaaatat caagcagttt atcaagctat
    25741 taaaaagaaa actaaaacaa gaagtggctg gaaagttgaa tacattaagg aggaataatg
    25801 gaagtttcaa ggacagaata tacaattaaa agagcaaaag agttatatga caatggagag
    25861 gatatattta ttgctataga taaggctaga gaaaaatatg aggagatgat taaaagtgaa
    25921 tatcttagct agtgtgatat tagtaatagg aagttttata gctggtagag tttatgagta
    25981 tagattgaac ctaaaagggt gtgaaaattg tgataataag aaggtgcaaa tatgaatgta
    26041 ttaagtctat tcgatggaat tagttgtggt caagttgctt ttgagagagc tagaataaaa
    26101 gtagaaaatt attttgcaag tgaaataaaa gagattgcga ttaaagtagc aatgaataat
    26161 tacccaaaaa caatacaact tggagatgta agagagctaa atgtaagtaa aatccctaaa
    26221 gtagatattc ttattggcgg aagcccatgc caaaatttaa gtaaaggaag attaatagct
    26281 aataaaatac aggatggatt aactggagat aaaagtaatt tgttttggga atatataaga
    26341 attctaaaac aattgaatcc agaatatttt cttttagaaa acgtagtgat gcccaaaaaa
    26401 gatgaaaaca tgataagcca attgttagga gttgaaccaa tcaagataaa tagcaattta
    26461 gttagttatc aaaatagaga cagattatat tggacaaata ttttaaatgt tcaacaacct
    26521 aaagatttaa aaattagttt tcaagattat aaagatacta gttttgatta ttgcaaagag
    26581 tttgaaatta aaaaaactcc aagtagagaa aaaatgtggg gtaatggttt aaatggaaat
    26641 tgtaagaatg taacatatga agacaaaata aactgcgtaa cattaaaaca agatagattt
    26701 agtaattcag gtttagtaga atttgggtct ttctgcagat acttaacaac tagagaatta
    26761 gagttagcgc agacgctacc agttggatat acaaaaggtt tgagtataag acaagcacaa
    26821 aatgtcatag gtgatggttg gacaatagat gtgatagcac atattttatc aaatattaac
    26881 tagttcttta tatataaatt attattgaag cggttagcta tcatgatact tgtagcagta
    26941 agttttatag ctggtagggt ttatgagtat agattgaatc tgaaagagtg tgaaaattgt
    27001 aataacaact atcctgaaaa atgagaaaga agtggtttta tgaataaaag aataatttgc
    27061 aattggtgtg gtaaattatt ttacatccca aaacagtcta aaaaaattta ctgttgtaaa
    27121 agatgtgaga gaaaggctaa gaaaagcaat agagaacagc aaaattaatt ttaaacaata
    27181 aaactatggg ggaatagcaa tgaataagtt tcaaaaagca gtttctcaaa tggtgaagca
    27241 agaggaaaaa gaaaacttgt tgcaaggata tgagaattgt atagtaggta gaagtatatc
    27301 aagttccata agaagatatg taaaagggtt tgaaaagttt ggatatagtg tacatgaggt
    27361 ttatgaattt ataaacgata ttaataagta tgagtaactt taagtgataa aggaagtatt
    27421 tgattgagta aatacaataa taagaaaatt gtaatagatg gaattaaatt tgatagtaaa
    27481 gatgagtcag agtattattt atatttaaaa gaaaaaaagg aaaatggaga aataaaagac
    27541 tttggacttc aacaaaagtt tgaactacaa cctaaattta aaaaagatgg gaaaagctat
    27601 agagctatta catatacaat tgattttgcc atatacaaat ggaatggtga agtcgtttat
    27661 atagatgtga aagggtatag tacacagcag ggtgaactta gaaaaaagct ttttgactat
    27721 aaatatcagg acaaaaaatt gatatggatt gctaaaagca aaaaatatgg ggtagatggt
    27781 tggatagaat atagtgaact taagaaaaag agaaaagaaa ataagaaaaa ggtagcttaa
    27841 ataaatagga gtgatgttat ggcaagtaaa gttaaaaagg agttttttat ggcaactaaa
    27901 aaacaccttg agaactacaa acaactacat attaatattg aaagtctaaa acttcaaata
    27961 aaaaatctca aagagttcca tttaggtgat tttatgcaag gtttaagcta tgacagcatt
    28021 cccataagta agactaattc aataagtaat caagttgaaa atgagttaat taatcttgaa
    28081 gaaaagataa tagaaaagca gatagaatta tatgaaatgg aagcgctaaa atatacaata
    28141 gatgtatcca taagtaattt aaaacctata cataaacaaa ttataaggta taggtatatt
    28201 gaaggcttag aatggagttt aatagttgat aaagtatact tagaagaaag acaattaaga
    28261 gaaagagcta atcaagccat tagttcaata tcaatagcct tatttggtaa aaaagcatta
    28321 atagagcaag aaccattatt taagatgtta gatttatagg cagttaataa ctgtctattt
    28381 tttttgtgaa aaagatatat ggaagttatt gacaatatca cgcatacgtg atattattga
    28441 agtatagaaa ggaggcgaaa agaaagtggc taagaaaata aaagagttca gaaaactcat
    28501 aaaagaatta actgaactcg tactcgaagt tggcacacta ttagcagtca tcaaaatggt
    28561 aatagatagc ctacattagt aaaatgttaa gagtggaagt tgcaccttcc ctcttaacta
    28621 aattataaaa catagtcact aaaaatacaa tgaataagta tagagagtta acatttgaat
    28681 tattgaaact actgtatgac actattaaat taattggagc atcaatattg ttatattatg
    28741 ttattaactc aattttctag caatgattag gaggtatttt atgaatcgat ttgaaggtat
    28801 ttatagcttt gcagaagcaa caaaattatg ggatttgaaa gattctacac ttagaaaagc
    28861 agttgcgaca ggcaaattag ttgaaaatat tgattgtaaa aagtttggtc gtgattgggt
    28921 agtaactatt gaagcaatgg aaagagaata tggaaaatta aataatataa tgaaagacct
    28981 agattaaatt ctaggtcttt ttattgccgc ttttctgccg attttacaat ttaaaatgtg
    29041 agataatagt attgtggaaa tgaatatttc tctctcaaaa ctaaatatat gtgggctagg
    29101 ttaagggatt cgcctagctt atatgaacaa actaggcagg gcatgaggat gctgtaagtt
    29161 caattcttac tatgttcaaa cttattaata cactatatgt agatatgctg gattaaaacg
    29221 gaatttaatt caaatgtcta aaagagtggg gcttggtaac ctcactcaat ttgcaaggac
    29281 tggtgtgtaa tcttaggttc gattcctaaa acttgctccc ttaaatataa tatgtatccc
    29341 caaaaaaagg cttagattaa attctaagtc tttttttaat aattaatgta gataataata
    29401 tttattgcta aaatgatgta caaatatgat aatataaatt acataaagat atataaatag
    29461 agggggatta taaatttgtt aaatgaaaag aatctaaaga acataaagaa tatattacaa
    29521 atggtacaaa tgaaacacct taaagatttt gaaaattggg gaattaataa acgaaaaaaa
    29581 gaaactgcta taaatagctt gataaacata atacaaaatg atggagaaaa actagattat
    29641 tttaaggaat ggatatcaat ctttacaata gatggtcata ataattatta tgtttttaat
    29701 tacaatgatt ctgacttgga attggaagat ttaaaaaaca tagtagaaga tatagtaagt
    29761 gaaaatttat tggagattga tactagtgat ttagaagaaa ctaaggtcat atataaaaaa
    29821 aatgatgtca taaacaaaag attgattata agatttattt ctccagcttt aattagctgt
    29881 aaaaatgaaa atgagaatgg gaatatagaa tctggattag aaaaaacttt ttattttgca
    29941 actttatttc tagacttaag tttgaaacaa ataatagttt ctataccaca tactgtaggt
    30001 ataaaaagca tagataatat agaggctaaa gcaagagatt tctcagaaat agcaaaatat
    30061 tatgtatcaa aattagaaga tttgttaact acttataata tacaaataga aaaatacaat
    30121 gattggatat atgatgctac atatgagctt gctgaggaag gcagtgctca taataatcct
    30181 gagataaaca aaaaatatga agaaaatatt gataaaatag atgaattcgc aaaaaagata
    30241 gctattgctt ctggaataac tgataaggct ataatagaaa atttcaaaga gggagtggga
    30301 atactctatg agaatattct aatagacgaa tttggtgtgg tagaggatga aaatcaatac
    30361 agtacttttg tgcaaaatgg tgatggagta aattcatact gtagagttgg gtctaaaaca
    30421 gcaaccctaa aatcaggaaa gggacatgct atagccaaga catctagaaa caatgaggat
    30481 gtcaagaatt taggtgtcat gaaatcattc aatggaactt tatccagatt tttaattgaa
    30541 gtcttagata aagaattata tttaataaga acagacactt ctaaatttat agaagagagg
    30601 gtgatttatg atgttatacg aaaagttgga gaatataagt atacttttag gaaatcagag
    30661 taaagcaaaa tctataatag aagaattaga gaaattaatg aagagtgata tcattacacc
    30721 aagattgctg gccaggaaaa ctaaagttac acttttagag gctacaagag ttcttgaatt
    30781 tttagttaaa gaaaatgaat tagaattttt tattgttgtt gaatgttcta atccagatag
    30841 cgtatatgaa aatgaaatag gtcactataa atacttcact tcgataaaag aattcaatgc
    30901 tttttcaaaa ggacaagaat gcccaatatg tggatgtggt tataaataca actttgaaga
    30961 tacaaaaata ggctttagaa gattggagaa gtgatacagt gagtctagat accttaaaag
    31021 aatatctaag catatgtgat gataatttaa tttgggagcc tttttatgat gacagtatgc
    31081 tattagacga attctctaat tatataaaag aaatagaatt atctaaggaa tggaataaat
    31141 ctaaaaatca tgagaaagga aaaattttag aacaattagt aagatttata atgtctagat
    31201 ttacaatatt aagtgatata tctgtaaata agtctacaac agataatgag ctagatgttt
    31261 ttgttaagtt taatgacaac ataccaatac cattcttatg taatataaaa tcaaaaatag
    31321 tatgtgaatg taaaaactgg aaaagtagaa aagtggatgt aggaatggta tctaaactag
    31381 cagaaatatg caataaaaat aatgcgggat tgggcatatt tatttcttta aatggattga
    31441 caggtaaggg gtggcaatat gctgaaggaa aaagaagaaa attatattta tcagataaga
    31501 tgccaataat atcgtttaca gtagatgaaa taacgaaatt aaagtataaa aggtataatc
    31561 ttctgactat gataaaatgt aaaatgcaag cattaataga tgagatggaa tttgctggag
    31621 gagaattaag attattagaa agtgaacttg gatttataga ctgtttaaat gaaaatatag
    31681 aatctttgaa aatgttgggt ttaataaccg aagttgaata taacaatatt aaagtaagaa
    31741 tcaatcatag atatgatatt gtaaactgag aactccaaac gagttctttt tttattccca
    31801 aaacgacaaa caaacgaggt ggtggtgtgc aagatgtgaa agaaaaagta aaacaagatt
    31861 acctaaaagg tatgaaacaa aaggaaatat cagcaaagta tgacattagt ttaaatactt
    31921 taaagtcatg gataaaaaga tacaattggg ctaatgagaa aaagaagggt gcacctaaaa
    31981 ataaaagagg tgcacccata ggtaataaaa atgccactgg tcctcctgga aataagaatg
    32041 ctgaaaaatt tggtttcttc tcaaaatact tacctgaaga aacccaagac ttaattaatg
    32101 agataaagga taaagataaa tttgatattc tttgggaaca gataacaata caatatgcag
    32161 caataataag agcacaaaag ataatgtatg ttaaagacaa ggaagaaatg attaaggaat
    32221 taaagaaata tgaaagcaca gaaaatggtg agaagataga gtatgaattt caatttgcat
    32281 gggataggca agcatctttt cttaatgcac agagtagagc aatgagtgaa cttagaagtt
    32341 taattaaaca gtatgatgaa atgattcata aggattggaa tttggctaca gaggagcaga
    32401 aaacaagagt tgagaagttg aaatgtgaag ttgataacct aagtaaagat gatattggag
    32461 atgatgagtt gaaaataagt gtagattatg gtgatagaaa tgatagttag aatagatttt
    32521 aatccagatt tcaaggaagc
//
LOCUS       DAEPIN010000048        32282 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.048, whole genome shotgun sequence.
ACCESSION   DAEPIN010000048 DAEPIN010000000
VERSION     DAEPIN010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 32282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32282
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.048"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            complement(<1..686)
                     /locus_tag="KR250_002406"
     CDS             complement(<1..686)
                     /locus_tag="KR250_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBH0068335.1"
                     /translation="MNRNNFSHEIIEPIDNLDVNFKLFDDSGSYVANHWHNSLEIIYI
                     TSGDLQINMEGYTYNLKANECMFINSGIIHSTRCIYQNTSILLQVPLSFLNKYIPDFK
                     NCYFDFKVNANDNNYKKNSSKVKLILENMREIKLSSPPAANLLFTSLLFELLFELYTN
                     FKISVGNKNMKKTVLDLSKFEPVLEYTKINYKMPISINKIAKVAHLQPEYFCRKFKQY
                     MGQTYLEYLN"
     gene            903..2069
                     /locus_tag="KR250_002407"
     CDS             903..2069
                     /locus_tag="KR250_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HBH0068336.1"
                     /translation="MYIKGVNLGGWLVLEKWMTSSLFEGTEAEDEYYLPRQLSREAYE
                     SRIKTHRSEYITERDFATIKSMGFNSVRIPVPYFIFGDCEPFIGCVKELDKAFAWADK
                     YGLSILIDLHTVPGSQNGFDNGGISGICSWSQNPEYVAFTLNVLERLAKRYGMRHELY
                     GIQILNEPITERMWNIMNVPNRFKAVDKEMARGSKPNSLEFLRDFYIKAYRVMRPYMR
                     EENVIVFHDAFELKAWKDFMREEEFKNVVLDTHQYLMLAEADGCEQSIDSYLKYIREN
                     YAKDILQMQKYFPVICGEWSLFNSYVCGIDTNGGQSPLNGIESNIDKLSKDDKRELYR
                     KIAKAQLDAWRNGSGHYYWNYKLLLDTVNEEGWIGWDSWDLGKCVAQEWYPIEY"
     gene            2091..3737
                     /locus_tag="KR250_002408"
     CDS             2091..3737
                     /locus_tag="KR250_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HBH0068337.1"
                     /translation="MIKNPILPGFNPDPCICRKGDDYYLVVSSFEWFPGIPVYHSKDL
                     KNWELYTHILTDETKIDLKKLPSSKGIWAPCLTYCEEEDLFYIVYGIMNSMNARYFDV
                     DNYLITSKDIKGEWSEPVYLHSSGFDASIFHDDDGKKWIASLDWETREGYEKPGVICL
                     VEYCTKKKEIVGYPKRIWSGGTDRGCIEAPHITKRGDYYYIMCAEGGTGYGHSVTMGR
                     AKNIWGPYEKDSMNPIVTSIPGDFYERHDPDHLKPKYYNPESKLQKSGHGSYIETTSG
                     EVYLVHLTSRPFVPELRCTLGRETAIQKMKWTKDNWLRMEDESNLAKEYVSESKLEEH
                     LVSSIPSFDDFDSNELGLQYYAPRISPLSFADVKSRPGYVRIRGQESRTSLNKVSILA
                     RKLTSVYARITTKMEFYPEVHQHSAGLIMYYDNMNYINLRKYYSETLGQSALSIIHLE
                     NGEKTEFLNTRIPIKDIPIYLRLYIQGRKSYFEWSYDEKNYQRIGKVFDTTKFSDEYC
                     KYGEFTGTFIGLTCADRVKHKHYADFDFFEYIVDESKDVD"
     gene            3834..5207
                     /locus_tag="KR250_002409"
     CDS             3834..5207
                     /locus_tag="KR250_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBH0068338.1"
                     /translation="MNTEVNVKATTIRYEKISFFERISYGCGDLGCNIIYSAMSAFLL
                     FYYTNYADVSAAAVGSIMLVSRILDGFSDLTMGIIVDRTKSKYGKARPWILRMAIPFA
                     IAAVLLFSVPSNLGITSKLIYIFITYNLVSTVIYTAINVPYATLNSLITQNQYERGVL
                     SIFRMILATCDTLLINGLTLPLVEYFGNNLSAWTKTFFVFGIASIMVFFITFTGTKER
                     VKAVKQDRNEVIPFKIGIKSLFRNKYWIQITLCLVCIFIVFAINGGSSVYYAKFILGD
                     EKLFGPINMVSNISQIIAMFMVAPFIKKFGKRNVLIVGSIILISSNVMFIIVGQNHIG
                     VIFASAIKGIGSAGIASTMFAIVSDTIEYGEWKTGYRTEGLINSASSFGFKVGNGLGS
                     AILGAVLSIGGYVGTSATQSDLAIVSIKVCFIYLPIFITILQIIIMSFYKLDKEYSTI
                     LNELNTK"
     gene            5652..6605
                     /locus_tag="KR250_002410"
     CDS             5652..6605
                     /locus_tag="KR250_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HBH0068339.1"
                     /translation="MKKIGDLRLMMKCCSLYYEDNLNQQEIANQLGISRPTISRILKE
                     AFEQGIVKIQIVDVLKNDYQKIERSLERKYKLKEVIVVDDKQDALTQKQELARAVSEY
                     LTRVVKENDIIGVSIGTTLKEIPRYVEKSNCKNVTFIPLLGGIGDNEIDIHANQIAVS
                     LARAFGGDFKLLHAPAVMSDLSTKEKLCKDEKIKEVLDLIDKTTIAIVGIGNPMSLNS
                     TIMASGYMNEADIEDLKKYNSIGAICLQAFDKEGKTSILEFNQRVLGVKLEDLKKIKR
                     TIGVASGDEKIEAIKASLKAKFINSLAINHSLALKLLEDCD"
     gene            6643..7605
                     /locus_tag="KR250_002411"
     CDS             6643..7605
                     /locus_tag="KR250_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HBH0068340.1"
                     /translation="MSISKETLLEMYKRMNQARKFEEKVSWFFARGMVHGTTHLSVGQ
                     EASSVAAVMALEKGDLVSLTHRGHSQFIGMGIDLNKMMAELMGKETGFCKGKGGSMHI
                     ADIESGNLGANGVVGGGLTIAPGAALTQQYKKTGKIVLCSFGDGASNEGTFHEGINLS
                     SIWKLPIIFYCENNLYGMSTSIKRHMNIESIATRAASYGIEGISIDGYNPIEVYETVQ
                     KAAEKCRRGEGPVLIESRTYRWLGHSKSDANVYRTKEEIESWKAKDPIEFLKNYLIEN
                     NLSNEDELDKIQEFAKQSIEDAVEFAQNSPNPKIESLLEDVYAD"
     gene            7630..8616
                     /locus_tag="KR250_002412"
     CDS             7630..8616
                     /locus_tag="KR250_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HBH0068341.1"
                     /translation="MSTRELTYAQAIKEAMSEEMRRDENVIFMGEDIGIYGGAFGVSV
                     GMIDEFGPERVRDTPISEAAIAGAAAGAAATGLRPIMEVMFMDFVTISMDAIVNQAAK
                     MRYMFGGKAQVPMVVRCPGGSGTGSAEQHSQSLEAWFCHVPGVKVVAPSTPADAKGLL
                     KAAIRDNNPVIFVENKLLYRKKGFVPEDDYVIEIGKADIKREGTDVTVITYGRMLQSV
                     EEAAETLSKENINVEIIDLRTLYPLDKETIVKSVCKTGRVLICHEAAKTGGLGGEISA
                     LITESESFDYLDAPVKRICGKDVPIPYNPELEKAVVPRVDEIEEAIKSLIVR"
     gene            8629..9675
                     /locus_tag="KR250_002413"
     CDS             8629..9675
                     /locus_tag="KR250_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HBH0068342.1"
                     /translation="MVANKIKATPAARSQARKDNIKLDRLIGSGENGRIHLVDVLNYL
                     KDNKANTTPLARRIAEDLNIDLETIVGTGYNGKIRKCDVEKLTGKETIVSTNTSKSSE
                     KKELKIENENSRMFNTVEGIFEKPNPMRATVAKRMSESYFSAPVFTFNIEVDATELKV
                     LRAKLIDTVKESTGVKLTMTDLIVMAVSKILPNHQALNSAWTDEGIFRYKDVNIAIAV
                     GLDEGLYVPVVKNANKKSLKEIAKESKELAEKVKTGKLMPADQEGNTFTISNVGMYGI
                     TTFTPIINMPSSAILGVGATQDKFVPVNGEAKIKPIMNLSLTSDHRVIDGTVAAKFLK
                     DLKELLENPLSMLV"
     gene            9842..11572
                     /gene="lpdA"
                     /locus_tag="KR250_002414"
     CDS             9842..11572
                     /gene="lpdA"
                     /locus_tag="KR250_002414"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HBH0068343.1"
                     /translation="MSVEVIMPKAGVAMEEGTIVSWLKQEGEEVKIGEPILEITTDKV
                     NMEIESEGEGTLAVIIHKEEGEVLPVFTVIGVIAEKGENQEEVKAKYLSGNVSKEDIV
                     EENQNIEFKEEKINKKECNHDYDVVVIGGGPGGYLSALKAALLGGRVALVEENILGGT
                     CLNRGCIPTKTYIKTAEILEEIDQLSKRGVKVTVDKEQDIKKAIKYKNRVVKKLTAGV
                     GGLLKSRDVDVFNLKASVKEEHKVILSDGKVLDTENIIIATGSKVRILPIKGIESNLI
                     ITSTEALDLETVPEELVIIGGGVIGCEFAEIFNSRGSKVTIVEMEDRVIPRMDKELSE
                     SLKYSLSKKGINVLTKKKVSEFKEEGNNILVCIEGEEPIKADLCLYAIGREANLSGIE
                     DLDIKIDKGSIVVNSKMETSIPSIYAVGDVTGGVMLAHAAFKMGEVAASNALGVNKEV
                     DLGALPSCVYTIPEVASVGITEEDARKKYNVKVGKFNFAGNGRALASGQEQGYVKVVA
                     DAKYGEILGIHMFGCGVAELINHAASFKALEIPTDEASELIFGHPCTSEALMEALADV
                     NGECLHLPKK"
     gene            11990..14938
                     /locus_tag="KR250_002415"
     CDS             11990..14938
                     /locus_tag="KR250_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBH0068344.1"
                     /translation="MRQRIKNLIENEDKKNPLTDEAISSRLNIRREDVTFLRNELKIE
                     DSRQRRKVVLIKAIKDILKEEKTINKNDITRRLNSIGFTVSRFTVIQYLKEIQDSGDL
                     KLDKESDDKKEEIINNRENKDIAFDKLIGSSGSLSTIIKLAKAAILYPPHGLHTIIMG
                     PTGVGKSELAECMYKFAVESSRFPKNAPFIVFNAADYSENPQLLLSQLFGHVKGAFTG
                     ADENKEGLVSKADGGILFIDEIHRLPHEGQEILFQLIDKGMVRKLGETRLTHKIDVMI
                     ISATSEPIDSHLLNTFKRRIPVTIEIPELVARPLNERFDIINNFFLIEAQRMGANIHI
                     KSDVLKALMLYDCIGNVGQLRSDIQVACARGLLNQLTNKLKEVNISISDVPGYVKQGL
                     IKIRNCRGKIENYVDGDLIIDSNLQGEIEKKNEDIYTFPDEIYKLTERRHIDLLEQGL
                     DFDVINRIIGGELESCIQKYIKQVKKVSISNNLPDISPIVGKYIVELTYEVIKIASQY
                     LSSLEPNLHLCLAVHLKAAFERLKEGKVISNAHLKEIIGNYPVEYSVAEKVAQYLKER
                     YNINLPKEEIGIIAIYFKMSSRRDMSEQKKIGVLLMAHGRVASSVCEVTNKLLGINHA
                     RYLDMPFEKKPEEMIEEATTIIKDIDEGRGVIILVDMGSLSYFGDIISGKTGINIRTI
                     SRFDTLLSIEVIRRAVLPDLTIDEIVDEVKGIEPQVKEKVSKCNNTKVDFRKPIILTL
                     CITGLGGACKIKKILEENIKSITEKVDIEPIGMIEYPSLDLEIKQISNDKNVIAIVGT
                     IDPQIANIPFFSLVDINDKAKLNQINNLIDTNQIGETNKKYELSDLIHKEDIFVDLQV
                     KSKEEVLKYMSKKLREKGYVTKNFYKKVVERDNLFPTELSDEIAIPHTDGIDVIRPAI
                     SIAILKKPIVWSKSKVKIILLLAVDQKCFNPLSTLLSFIETDEFKKIKNIKDRDFIRE
                     MILDGVNENN"
     gene            14916..15380
                     /locus_tag="KR250_002416"
     CDS             14916..15380
                     /locus_tag="KR250_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBH0068345.1"
                     /translation="MESMKIISSNLIFKNIEVSNNEDALKFLGQRLFDEQYVKESYIQ
                     AVVAREKKYATGLPTEIYGVAIPHTDIVHVNEPGIAIGILNKPVKFIMMGTDDTEIDV
                     KVVFMLAVKEPQEQLQLLEKLMTIFQDKNILNNIVDLSEESVSDLLNSKLKN"
     gene            15493..15795
                     /locus_tag="KR250_002417"
     CDS             15493..15795
                     /locus_tag="KR250_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBH0068346.1"
                     /translation="MGVTNVAKKILVACGTGVCTSTIAINKLKKALQDIGKLDMVNIT
                     QCKVVEVASKAPDYDLIICTTQVSSSIKTPVINGLPFLTGVGMDKLINDVLEELEL"
     gene            15857..17221
                     /locus_tag="KR250_002418"
     CDS             15857..17221
                     /locus_tag="KR250_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425524.1"
                     /note="with GatAB forms a phosphoenolpyruvate-dependent
                     sugar phosphotransferase transporter for galactitol;
                     subunit IIC forms the translocation channel and contains
                     the substrate binding site; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBH0068347.1"
                     /translation="MEGLYMEILNFIVGLGAQVMMPIIICIFGLILGTKLGKSLRAGL
                     TVGVGFIGLNTIIALLTDNLGPATQQMVKNLGLSLSIIDVGWPAASAIAFASTVGALI
                     IPIGLVVNIVMLITNTTQTVDVDIWDYWHFAFTGALVAGATQSVMWGVFASVANMVIV
                     LVMADLTAPGIEEYLGMPGISLPHGFTQAFVPIAIVVNKLLDLIPGINKIEINADTLQ
                     KKFGLFGEPLIMGSVIGVIIGIAAKYDIKGILQLGVTMGAVLILIPKMAALLMEGLLP
                     VSEAAQEFIEKRFKNRGKIYIGLDSAVGIGHPVTLSVALVLVPLTILIAAILPGNKVL
                     PFADLAVIPFALVLIVPITKGNVFRTLIIGIIIITSGLLIATNLAPLFTQMALNASFK
                     MPEGATMISSICDGANPLSWVFVKVMNYKVIGGVVFGVIALGMAIYNRNRIINENKKL
                     SIEE"
     gene            17256..17729
                     /locus_tag="KR250_002419"
     CDS             17256..17729
                     /locus_tag="KR250_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0068348.1"
                     /translation="MKKYKYDSKRYLLSVTIPGFFMILILIYALFNNYTNFNLNIYSL
                     LIIVCTYGIFNTFISSSNPKKIIIDSKCIHFTSFMTTHKYEIKDIEKIRIREFYNKKI
                     YVKINDGNLFKGRYWIRTNMFNNSIALYSYFIELEECLYPDSLKFRNKKFENKNI"
     gene            17901..18551
                     /locus_tag="KR250_002420"
     CDS             17901..18551
                     /locus_tag="KR250_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HBH0068349.1"
                     /translation="MLLEKLRKEVLQASLDLLNYNLVTLTGGNVSGRDEQTGYIAITP
                     SGMDYRNLTPSDIVIVDVDGNIIDGKWKASVDLSDHLYIYKHREDINSIIHTHSTYSS
                     CFAILNEPIECASTTLANEVGGSVPVAKFSPPTSKKMGKCVIEAIGDKRACLLANHGV
                     IAVGPSVGHALTAAVMLEDSAKVYYLAKSIGTPVLLPDEEIQRARDVFFNVYGQDK"
     gene            18725..19657
                     /locus_tag="KR250_002421"
     CDS             18725..19657
                     /locus_tag="KR250_002421"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HBH0068350.1"
                     /translation="MYYLLNKSTNPYFNLALEEYLFLNDKYNDDIIIIWRNEESIFIG
                     KNQNPYQEVYHDVIEKGEIPILRRISGGGTVYHDLGNINMSFIQKDRQLHEIDFLEHT
                     KFMQKMLSTLGLDVSITERKDLFLNGKKISGSAQSIKRKNSLYHGTLLYDSDLNKLTK
                     YLNSNKATESNATKSVSSKVTNIKFLLEKDINYFLDYCVEYLKSNIKNIKELELDEED
                     IKSVYNIGEDKYKKIEWTYGKTPKFQVVLPFNNESKINIHVNRWKVSKFFISYDDNVI
                     NMDKLLDLNFFKEEIKESILENYPHYIDLINLIF"
     gene            19931..20644
                     /gene="ispD"
                     /locus_tag="KR250_002422"
     CDS             19931..20644
                     /gene="ispD"
                     /locus_tag="KR250_002422"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HBH0068351.1"
                     /translation="MYSVIIVAAGSGRRMNLDINKQFIKLREKEIIAHTIQVFYENIN
                     IDEIVVCIKKEEEDFFKENIINKYNFKNIKIAYGGKERQDSIYNGLKKLDENCDIVLI
                     HDGARPFVDHRIINESIKVAKEKKAVVVGVPVSDTIKIVSDGTVQETPERNLLWAAQT
                     PQTFEYNLIIDAYEQAYKNNYYGTDDSMLVENIGQSVTMVMGSYENIKITSPEDLNIA
                     EQILNMEKRDEVNSRRRII"
     gene            20641..21126
                     /locus_tag="KR250_002423"
     CDS             20641..21126
                     /locus_tag="KR250_002423"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HBH0068352.1"
                     /translation="MRIGLGYDVHKLTEDRKLIIGGVEIPHDKGLLGHSDADVLIHAI
                     MDSILGALALGDIGKHFPDTDEEYKGADSMKLLEHVYNLITSKGYKIGNIDSTIIAQS
                     PKMAPYIESMRSNISKVLNTDIDNINIKATTEEGLGFTGAKQGIASQSICLLLLTSQN
                     N"
     misc_binding    21221..21429
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            21493..23208
                     /locus_tag="KR250_002424"
     CDS             21493..23208
                     /locus_tag="KR250_002424"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HBH0068353.1"
                     /translation="MKMSKMFMPTLKEIPADAEITSHQLMVRSGMIKKMTSGVYNQLP
                     MGLRVFKKIEQIIREELNKKDCQEILCAALLPSELWKESGRWTAMGEEMFRLKDRTER
                     EYCLGPTHEEAFTDIIRQEITSYKQLPLNLYQIQVKYRDERRPRFGVMRTKTFTMKDA
                     YSFDVDDKGLDKSYQDMFDAYVSIFDRCGLENSPVQADSGAIGGSTSAEFMVKSEVGE
                     DEVVFCSGCDYAANVERAESCNLASQKEEMKELEEVHTPGAATIKELEEFLKTSPDKF
                     AKTLVYEADGKTVVVVVRGDREVNEIKVSNAIGSVIEFALATDDVVRKVTNAEVGFAG
                     PIGINADYVFIDKEIVEQRNIVVGANKTEYHIKNANYGRDFEGIVGDFRNVQEGDKCI
                     VCGKPLEIARGVEVGHIFKLGTKYSESMNANFIDKDGKSKPIVMGCYGIGVERTAAAI
                     IEQHNDEKGIIWPLSVAPYHVVIVPANMKNEEQISIAENIYNDLQAMGVEVLLDDRDE
                     RIGVKFNDSELIGIPMRITVGKNINEGKVEFKLRHKEDKEIIDIEEINEKVKAEFIRN
                     NVRLG"
     gene            23330..24775
                     /locus_tag="KR250_002425"
     CDS             23330..24775
                     /locus_tag="KR250_002425"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HBH0068354.1"
                     /translation="MAKNEKQFVEEITKMEDDFPQWYTDVITKTDLVDYAPVKGFMVV
                     KPYGYALWEKMQEFMDKKFKETGHKNCYFPLLIPESLLNKEAEHVEGFAPEVAWVTHG
                     GNKKLEERLCVRPTSETIICTMYAKWLKSYRELPYLYNQWCSVVRWEKSTRPFLRTSE
                     FLWQEGHTLHETAEEAQEETIQQLEVYKALCEELLAMPVVAGQKSESEKFAGGERTYT
                     IEAMMHDGKALQSGTSHFLGQHFTKAFDITFADREGNLANPYHTSWGASTRLIGGLIM
                     THSDNRGLVLPPRVAPIQVVIVPIAAKKGNVMETVDKIYADLKAKGVAVEVDDRDNYT
                     TGWKFNEWEMKGVPVRVEIGPKDIENNQAMVFRRDTLEKDSMPLEGLADAICDLFDVI
                     HNDMFEKARKHREDNTSIVENMDEFRKALEEKPGFIKTMWCGDAECEAKIKEETGATI
                     RCLPFEQENLGHKCVYCGKEADSMVVMAKAY"
     gene            25215..26696
                     /locus_tag="KR250_002426"
     CDS             25215..26696
                     /locus_tag="KR250_002426"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HBH0068355.1"
                     /translation="MNVRVRFAPSPTGFVHIGSLRTALYNYLFAKKMGGEYILRVEDT
                     DQSRLVEGAIENMLNAMKWAGVNHSEGVILDDSGKVVQKGEYGPYIQSQRLDIYQEYI
                     KQLLDSGKAYYCFCTKERLDEVRDAQRAAGETAKYDGHCKNLSKEEVEANIKAGIPYV
                     IRLRLPENHTIKFTDLVRGDMEFNTNDLDDQVLMKTDGFPTYHFAVVVDDYLMKITHV
                     IRGEEWVSSTPKHVYLYEAFGWEAPVFVHLPNILNKEKKKLSKRQGDVAVEDFKKKGY
                     LPEGLVNYVALVGWSPEDNQELFTMEELEKAFSVERVSKSGGVFDTEKLNWVNQHYIK
                     DGDDAYLTDLAIPFLIEDGFITEEEATNKYDFLKSMISVLKEKLQYVKEVTEHASIFF
                     GDKIEVETEEGREFLRLEHIPTLIDALREKIEKTEVLNAEFVQAMLKEIQKEYKIKGK
                     NLFMGSRIILTGQMHGPDLPKVMEVLGKETCLNRIAYVKNNIL"
     gene            26917..27072
                     /locus_tag="KR250_002427"
     CDS             26917..27072
                     /locus_tag="KR250_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0068356.1"
                     /translation="MYSNIDDVKKELKELCLEYVTILEKLKDEKMITEETFEKCSSQK
                     KIFLEEQ"
     gene            27217..28614
                     /locus_tag="KR250_002428"
     CDS             27217..28614
                     /locus_tag="KR250_002428"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HBH0068357.1"
                     /translation="MKVYNTLTRTKEEFVPLEEGKVKMYVCGPTVYNYIHIGNARPFI
                     IFDTLRRYLEYRGYDVTYVQNFTDVDDKIINRSHEEGISPEEVAAKYIKEYFVDCDGL
                     GIKRATVHPQVTDNIQQIIEFIKELEDKGYAYAVNGDVYFDTNKFEGYGKLSGQKQED
                     LEAGARIEVNDQKRHPMDFVLWKAKKEGEPGWDSPWGEGRPGWHIECSVMSKRYLGET
                     IDIHAGGQDLTFPHHENEIAQSEARSGKTFSKYWMHNGYININDEKMSKSKGNFFTVR
                     DISKLYDLEIVRFFMLSAHYRNPVNFSDEMLNQAKAGLERLYNTKEKLEFTLSNLVES
                     PLTEKEVELVKELDDFRQKFIDAMDDDVNTADAVSVIFELAKLINSNVDENSSLEFAK
                     KCLDEFNELTGVLNIVNKKKDTVLDKDIEELIQKRTDAKKNKEFQLADDIRQQLLDMG
                     IVLEDTRQGVKWKRI"
     gene            28599..29012
                     /locus_tag="KR250_002429"
     CDS             28599..29012
                     /locus_tag="KR250_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HBH0068358.1"
                     /translation="MEKDIKMEKTELVTMSPLVLAYLGDTVYETYIREYLIRQNTQRK
                     VNDLHKLAIKYVKAKAQATIIHEIEIELTEEESKIYKRGRNQKSNTSPKNADIIDYKH
                     ATGFEALVGYLYLNNEIERLQYIINKGIKIIERDM"
     gene            29118..29876
                     /locus_tag="KR250_002430"
     CDS             29118..29876
                     /locus_tag="KR250_002430"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HBH0068359.1"
                     /translation="MKVKLISHTPEPEKVIAMAAKLCYSPVGTDEIEKDLTDESIEKF
                     LNMLLSIGHGSILEHASFTFSIEGISRACSHQIVRHRIASFSQQSQRYVKLEQFEYII
                     PPEIEKIEKAKELFIDSMKKDQENYDKLVEILFENHYNDLIKNGKNEKTAKRQAEKKA
                     IEDARYVFPNACETKMVFTINARSLFNFFEHRCCERAQWEIRNLAVEMLREVKKVAPI
                     LFKKTGPSCVNGSCPEGTMTCGDIVQVREKFKAL"
     gene            29889..30626
                     /gene="rlmB"
                     /locus_tag="KR250_002431"
     CDS             29889..30626
                     /gene="rlmB"
                     /locus_tag="KR250_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HBH0068360.1"
                     /translation="MASIEGRNPVIEAIKSDREIDKILIANSAKEGSIKKIIGMAKDK
                     NIIIQYVDKHKLDEVSTSHSHQGVIAYASEYKYYELDELIDLAKNKDEDPFFIILDEI
                     TDPHNLGSIIRTADAVGAHGVIIPKRRSVHITPVVAKASAGAVEYMPVCKVTNIVNTI
                     KRLKEEGLWIAAADMDGETFYKQNLTGPLGVVIGSEGFGISRLVKQNCDFIVKMPMIG
                     NVTSLNASVAGGILLYEIFRQRLDKSK"
     gene            30629..31156
                     /locus_tag="KR250_002432"
     CDS             30629..31156
                     /locus_tag="KR250_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HBH0068361.1"
                     /translation="MKKNINHYLIIDGYNIINAWDNLKELAKEDLEDSREKLIDDIIE
                     FSEFMGYKTIIVFDAYNVKNSREKVEKRKHITIVYTREHQTADSYIEKFITSLSKYDD
                     VKVATNDYAEQQMILGKGATRMSARELKLELDRSKNKMKEKNIGLRKKIQRNWLEERL
                     DKETLSKLENIRRKR"
     gene            31221..31868
                     /gene="sigH"
                     /locus_tag="KR250_002433"
     CDS             31221..31868
                     /gene="sigH"
                     /locus_tag="KR250_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigH"
                     /protein_id="HBH0068362.1"
                     /translation="MLVAKEKSYELVDNCQQDEYNIVLRASEGDKIALEYIITKYRNF
                     VKAKAKSYFLIGADKEDIIQEGMIGLYKAVRDFDGSKTNSFKCFAEICITRQIITAIK
                     TATRQKHIPLNSYVSLNKPIYDEESDRTLLDIIATSIVTDPEELIISKEELKNIESKM
                     NELLSDLELEVLELYLNGKSYQFIADKLKRDVKSIDNALQRVKRKLEKHLENRND"
     gene            31942..>32282
                     /locus_tag="KR250_002434"
     CDS             31942..>32282
                     /locus_tag="KR250_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HBH0068363.1"
                     /translation="MAKAKYERTKPHVNIGTIGHVDHGKTTLTAAITKTLYDRYQLGE
                     AVDFANIDKAPEERERGITISTAHVEYETPNRHYAHVDCPGHADYVKNMITGAAQMDG
                     AILVCSATDGP"
BASE COUNT        13132 a         3387 c         6112 g         9651 t
ORIGIN      
        1 tcatttaaat actctaagta agtttgtccc atatactgtt taaattttct acaaaaatat
       61 tctggttgta agtgtgctac cttagcgatt ttgttaatag aaataggcat tttgtaatta
      121 atctttgtat attctaaaac tggttcaaat tttgacaaat caagtactgt ttttttcata
      181 tttttattac ctacagatat tttaaagttt gtatataatt caaataaaag ttcaaataat
      241 aagcttgtaa agagcagatt agctgcagga ggagaactaa gtttaatttc tctcatgttt
      301 tctaatataa gtttaacttt acttgaattt tttttatagt tattgtcatt ggcattaacc
      361 ttaaagtcaa aatagcaatt tttaaaatca gggatatatt tgtttagaaa agacaatggt
      421 acttgcaata atatagaggt attttgataa atacatctag ttgagtgaat aattccagaa
      481 ttaatgaaca tacactcatt agcttttaaa ttataagtgt aaccctccat gttaatttgt
      541 aaatcaccag aagttatata aattatttct aatgagttat gccaatgatt tgctacataa
      601 ctaccactat catcaaatag tttgaaatta acatctaaat tatcaatagg ttcaattatt
      661 tcatgtgaaa aattatttct gttcataata atatcctcct tataatttta aattataaat
      721 agtaaattta taatgttcaa tattaaaaag ataatataga ctaataaatt agataataaa
      781 catacttaag aaaacggata aaatcaaata taataactgt atattaaaat aacttaaaaa
      841 aattatttta ttataagtgc aaaatatacc tatttaaata agataagagg ggaggatttt
      901 atgtgtatat aaaaggtgta aatttaggag gttggttagt tttagaaaaa tggatgacct
      961 caagtttatt tgaaggcaca gaagctgaag atgaatacta tttaccaaga cagctttcaa
     1021 gagaggcata tgaatctaga attaagactc atcgtagtga atacatcaca gagagagatt
     1081 ttgcaacgat taaatctatg ggatttaatt cagttcgtat accagtacca tactttattt
     1141 ttggagattg tgaaccattc ataggatgtg ttaaagaact tgataaagca tttgcttggg
     1201 cagataagta tgggcttagt atattgatag acttacatac agttccagga agccaaaatg
     1261 gatttgataa tggaggaata tcaggtatat gtagctggtc acaaaatcca gaatatgtag
     1321 cgtttacatt aaatgtatta gaaagacttg caaaacgtta tgggatgagg catgaattgt
     1381 atggaatcca aattttaaat gagcctataa cagaaagaat gtggaacata atgaatgttc
     1441 caaatcggtt taaggctgta gataaggaga tggcaagggg gagtaaacct aattcattag
     1501 agtttttacg tgatttttat ataaaagcat atagggtaat gcgtccttat atgagagagg
     1561 aaaacgttat agtgtttcat gatgcatttg aattaaaagc atggaaagac tttatgagag
     1621 aggaagaatt taaaaatgtt gttctagata cacaccaata tttgatgtta gctgaagcag
     1681 atggatgtga acaaagtatt gatagctatc tcaaatatat tagagaaaat tatgctaaag
     1741 atattttaca aatgcaaaaa tattttcctg ttatatgtgg ggagtggagc ttatttaatt
     1801 catatgtttg tggtatagat acaaatggag gtcaatcacc tctaaatggg attgaatcaa
     1861 atatagataa attatcaaaa gacgataaaa gagaattata taggaagata gcaaaagcac
     1921 agcttgatgc atggagaaat ggaagtggtc actactattg gaattataag ttattactag
     1981 atacagttaa tgaagaaggt tggataggat gggatagttg ggacctaggc aaatgtgtag
     2041 ctcaagaatg gtatcctata gaatattaaa ctaaaattgg aggaaaagat atgattaaaa
     2101 accctatact tccaggtttt aatccagatc cttgtatttg tagaaaggga gatgattatt
     2161 atcttgtagt ttcatctttt gaatggtttc caggtatacc tgtttatcac tctaaagact
     2221 taaaaaattg ggagttatat actcatattt tgacagatga aacaaaaata gatttgaaga
     2281 agcttccttc atctaaagga atttgggcac cctgtttgac ttattgtgaa gaagaagatt
     2341 tattttacat agtttatgga attatgaact ctatgaatgc aagatatttt gatgttgata
     2401 attatttaat tacatcaaaa gatataaagg gagagtggag tgaaccagta tatctacact
     2461 catcaggttt tgatgcgtct atttttcatg atgatgatgg taaaaagtgg attgcatcat
     2521 tagactggga gactagagaa ggatatgaaa aacctggtgt tatatgttta gtggaatact
     2581 gtacaaaaaa gaaagaaata gtaggatatc caaagagaat atggtcaggg ggaactgata
     2641 gaggttgtat tgaagcacca catataacaa agcgtggaga ttactattat ataatgtgtg
     2701 cagaaggtgg aactggatat ggtcatagtg taactatggg aagggctaaa aatatatggg
     2761 gaccttatga aaaagattca atgaatccaa ttgtaacttc aattccaggc gatttttatg
     2821 aaagacatga ccctgaccat ctaaagccta aatactataa tccagaatca aaacttcaaa
     2881 aatctggtca tggaagctat atagaaacaa catcaggtga agtatattta gttcatctaa
     2941 catctagacc atttgtacca gagttaaggt gtactttagg tagagaaaca gctattcaaa
     3001 aaatgaaatg gacaaaggat aattggcttc gaatggaaga tgaaagtaat ttggcaaaag
     3061 aatacgtatc agaaagtaaa ctagaagaac atttagtttc atctatacca agttttgatg
     3121 actttgattc aaatgaatta ggtttacagt actatgctcc taggatttca ccattatcat
     3181 ttgcagatgt taagtctaga ccaggatatg taaggattag aggacaagaa tcaagaacat
     3241 cattaaataa agtaagcata ttggcaagaa aattaacttc agtatatgca agaataacca
     3301 ctaagatgga gttttaccca gaggtacatc agcatagtgc gggtttgata atgtattatg
     3361 ataatatgaa ttatataaat ttaagaaagt attatagtga aacattgggt caaagtgcac
     3421 tatcaataat acatcttgaa aatggagaga aaacggaatt tttaaatact aggataccaa
     3481 taaaagatat accaatatat cttcgtctgt atatacaagg tcgtaagtca tattttgaat
     3541 ggagttatga tgaaaaaaac taccagagaa taggaaaggt atttgataca actaagtttt
     3601 cagatgagta ctgtaaatat ggagaattta caggtacgtt tataggatta acttgtgcag
     3661 atcgtgtaaa gcataaacac tatgctgatt ttgatttttt tgaatatata gtagatgaat
     3721 caaaagatgt cgattaataa aatcattact aaaattttca gagcaaaggt aaatcttatg
     3781 ctctgaaata agaaacatta gaaggcaaat cttacaaaaa ggagtatggt attatgaata
     3841 cagaagttaa tgttaaagca actacgatta gatatgaaaa gatttcattt tttgaaagaa
     3901 tttcttatgg ttgtggggat ttggggtgca atatcatata ctcagctatg tcagcatttt
     3961 tgctgtttta ttatactaat tatgcagatg ttagtgcagc agctgtaggt agtattatgc
     4021 ttgtatctag gatactggat gggtttagtg acttaacaat gggaattata gtggatagaa
     4081 ctaaatccaa atacggtaag gctaggcctt ggattttgag aatggcgatt ccttttgcta
     4141 tagcagcagt attattattt tctgttccat caaatcttgg tatcacttct aaattaattt
     4201 atatttttat tacatataac ttagtttcta ctgttattta tacagcaatc aatgtaccct
     4261 atgcaactct aaattcactt ataactcaaa accagtatga aagaggagta ttaagtatat
     4321 ttagaatgat attagccact tgtgatacct tactaataaa tggacttaca ctacctctag
     4381 ttgaatactt tggaaataat ttatcagcat ggactaaaac attctttgta tttggaatag
     4441 catctataat ggtatttttt ataacattta ctggaaccaa ggaaagagta aaagcagtta
     4501 agcaagatag gaatgaagta ataccattta aaataggtat taaatcttta tttagaaata
     4561 agtactggat acaaataaca ttatgtttag tttgcatatt tattgtattt gcgataaatg
     4621 gtggttcatc cgtatattat gcaaagttta ttttgggaga tgaaaaatta tttggtccta
     4681 taaatatggt ttcaaatata tcccaaatta tagctatgtt tatggttgct ccttttataa
     4741 aaaaatttgg aaaaagaaat gtattaatag taggttcaat aatattaata tcgtcaaatg
     4801 ttatgtttat aatagttgga caaaatcata taggagtaat atttgcaagt gctataaagg
     4861 gaataggaag tgctggaata gcatcaacaa tgtttgctat tgtttcagat actattgaat
     4921 atggagaatg gaagactgga tatagaactg aggggcttat aaatagtgca tctagttttg
     4981 ggtttaaagt gggaaatgga ttaggttcag caatattagg tgcagtatta tcaattggtg
     5041 gttatgtagg aacatcagct actcaaagtg atttagctat agtatcaata aaagtatgtt
     5101 ttatatacct acctatattt ataacaatat tacaaattat cataatgtct ttttataagc
     5161 ttgataaaga atatagtact atacttaatg aattaaatac taaataatga aaaagtttct
     5221 tataaagtct taagtttaat taaggtctat tgtaaatgtt ccctataaaa actattatat
     5281 atttatgatt attgaagaat ttttagagaa gcgaataata aaaatataag aagactttaa
     5341 atgatgaaat ggttggtttt ttagaaatga tttaataagt tattgttatt taaagcatta
     5401 taagtggaca attaattttt aatttatgga gatgatattt aacaattaga agtaatatat
     5461 aatatgcact aagaagcaat ttttcaacta tttatgaagg tggtggtaca agaaaagtat
     5521 cacctttttt ttgttataca aaaaaacagt tgacttttta tatgaacccc ttctataatg
     5581 ttactgaaat taagtaaaat gattgaacaa atgtaaagat aattgaaaag cttgattttg
     5641 gaggaaagca aatgaaaaaa attggagact taagattaat gatgaagtgt tgttcacttt
     5701 attacgaaga taatttgaat caacaggaaa tagcaaatca attgggaata tcaagaccaa
     5761 caatatcaag aatattaaaa gaagcttttg aacaaggaat agtaaaaata cagatagttg
     5821 atgttttgaa aaatgattac caaaaaatag agagaagttt ggagagaaaa tataaattaa
     5881 aagaagtaat agtagtagat gataaacagg atgcactaac acaaaaacaa gaactagcaa
     5941 gagctgtatc tgaatattta actagagtag taaaggaaaa tgatataata ggtgtttcaa
     6001 taggaacaac actaaaagaa ataccaagat atgtggaaaa aagtaactgt aaaaatgtta
     6061 catttatacc tctattagga gggattggag acaatgaaat agatattcat gcaaatcaaa
     6121 tagccgtaag tttagcgagg gcttttggag gtgattttaa attattacat gctccagctg
     6181 ttatgtctga cttgtctaca aaagagaaac tttgcaaaga cgaaaaaatt aaagaagtac
     6241 tagatttaat agataaaaca actatagcaa ttgtgggtat aggaaatcct atgagtttaa
     6301 actcaacaat aatggcttct ggatacatga atgaagcaga tatagaagat ttaaaaaaat
     6361 acaactcaat aggtgcaatt tgtttacaag cctttgataa agaaggaaaa acttctatac
     6421 ttgaatttaa tcaaagagta ttaggagtga aactggaaga tttaaaaaag ataaaaagaa
     6481 caataggtgt cgcttcagga gatgaaaaaa tagaggcaat aaaggcatca ttaaaagcga
     6541 aatttataaa tagtttagca attaatcaca gtttagcact taaattatta gaagattgtg
     6601 actaatagaa agatagataa aagttaacag gagggaatgt atatgtcaat atcaaaagaa
     6661 acattgcttg aaatgtataa gagaatgaat caggctagaa aatttgaaga gaaagttagt
     6721 tggttttttg caagaggtat ggttcatgga acaactcact tatctgtagg tcaagaagcg
     6781 tcaagtgtgg cggctgtaat ggctttagaa aaaggagatt tagtatcatt aactcataga
     6841 ggacatagtc aatttatagg aatgggaata gacctaaata aaatgatggc tgaacttatg
     6901 ggtaaagaaa ctggattttg taagggaaaa ggaggctcaa tgcatatagc agatattgag
     6961 tcaggtaact taggagcaaa tggagttgta ggtggaggtc ttacaatagc tccaggagct
     7021 gcacttacac aacagtataa aaaaacaggc aaaatagttt tatgtagttt tggtgatgga
     7081 gcatcaaatg aaggtacttt tcatgaagga ataaatttat cttcaatatg gaaattgcca
     7141 ataatatttt actgtgagaa taatttatac ggtatgtcaa caagcataaa acgtcatatg
     7201 aatatagaaa gtattgccac tagagcagct tcttatggaa tagaaggaat atcaatagat
     7261 ggatataacc caatagaagt atacgaaaca gtacaaaaag ctgctgagaa gtgtagaaga
     7321 ggagaaggac ctgtattaat tgaaagtaga acttatagat ggttaggtca ttcaaaatca
     7381 gatgcaaatg tatatagaac aaaagaagaa atagaatcat ggaaagcaaa agaccctatt
     7441 gaatttttaa aaaattatct tatagaaaat aatctatcta atgaagatga attagataag
     7501 attcaagaat ttgcaaagca atcaatagaa gatgcagtag agtttgctca aaatagccca
     7561 aatccaaaaa tagagtcttt attagaggat gtatatgcag actaagaatg gtaaaggagg
     7621 aaaataaaaa tgagtacaag ggaattaaca tatgcacaag caataaagga agcaatgtca
     7681 gaagaaatga gaagagatga aaatgtaata tttatgggag aagatatagg aatatatgga
     7741 ggagcatttg gtgtatcagt tggtatgata gatgaatttg gaccagaaag agtaagggat
     7801 actcctatat cagaagcagc tatagcagga gcggcagcag gtgcagcagc aactggctta
     7861 agacctatta tggaagtaat gtttatggac tttgtaacaa tttctatgga tgcaattgta
     7921 aatcaagcgg caaaaatgag atatatgttt ggaggaaaag cacaagttcc aatggttgtt
     7981 agatgcccag gaggttctgg aactggttca gcagagcaac attcacaaag tttagaagct
     8041 tggttttgtc atgtaccagg agtaaaagta gttgcacctt ctacaccagc agatgctaag
     8101 ggattgttga aagcagctat aagagataat aatccagtca tatttgttga gaataaattg
     8161 ttatacagaa aaaaaggttt tgtgccagaa gatgattatg taattgaaat tggaaaggca
     8221 gatattaaaa gagagggtac tgatgttaca gtaattactt atggaagaat gttacaaagt
     8281 gttgaagaag cagcagagac tctatcaaaa gaaaatataa atgttgaaat tatagattta
     8341 agaactttat atccattaga taaagagact atagtaaaga gtgtatgtaa aactgggaga
     8401 gttttaattt gccatgaagc agctaagact ggtgggctag gtggagaaat atcagccctg
     8461 ataacagaaa gtgaatcatt tgattattta gatgctccag taaaaagaat ttgtggaaaa
     8521 gatgtgccaa taccatataa tccagagtta gaaaaagcag ttgtcccaag agttgatgaa
     8581 atagaagaag caataaaatc tttaattgtt agataaggag ggatagatat ggtggcaaat
     8641 aaaataaaag ccacaccagc agctagaagt caggcaagaa aagacaatat aaaattagat
     8701 aggctgattg gaagtggaga aaatggaagg attcatttag tagatgtgtt aaattattta
     8761 aaagataata aagcaaatac tactccttta gctagaagaa tagcagaaga tttaaatata
     8821 gatttagaaa ctattgtagg tactggatat aatggaaaga taagaaagtg tgatgtggaa
     8881 aaattaactg gaaaagaaac tattgtatca actaatacat ctaaatctag tgagaaaaaa
     8941 gagctgaaaa ttgaaaatga aaattcaaga atgtttaaca cagtagaagg aatatttgaa
     9001 aagccaaatc caatgagagc tactgtagca aaacgaatgt cagaaagtta ttttagtgct
     9061 cctgtattta cattcaatat agaagtagat gctacagaat tgaaggtact tagagcaaag
     9121 ttaatcgaca cagtaaaaga atcaactgga gtaaaattaa ctatgacaga tttaatagta
     9181 atggcagtat ctaaaatact tccaaatcat caagccttaa attcagcatg gacagatgag
     9241 ggtatattta gatataaaga tgtaaatata gctattgcag ttggtttaga tgaaggctta
     9301 tatgttccgg tagtaaaaaa tgcaaacaaa aagtctttaa aagaaatagc aaaagaaagc
     9361 aaagaacttg cggaaaaagt aaagacaggg aaacttatgc cagcagacca agaaggtaat
     9421 acatttacaa taagtaatgt tggaatgtat ggtataacta catttacacc tataataaat
     9481 atgccttcaa gtgcaatctt aggggtaggt gctacacaag ataagtttgt tccagtaaat
     9541 ggagaggcta aaataaaacc tataatgaac ttatcattaa catcagacca tagagtaata
     9601 gatggaacag tagctgcaaa attcttaaaa gatttaaaag aattgttaga aaatccttta
     9661 tcaatgcttg tataggtttt gatattttgt tactgatata aatagtttga atagattata
     9721 ttattataat tgtttcagta aatttaaagc ttttatatat taagaattat ttttaacagt
     9781 aactatatgt gaatatgatt tgcttaatta aaatatatga aaataaatag gaggagagcc
     9841 tatgtcagta gaagtaataa tgccaaaagc aggagtagcc atggaagaag gtactatagt
     9901 atcttggcta aagcaagaag gcgaagaagt aaagattgga gaacctatac tagaaataac
     9961 aacagataaa gttaatatgg agatagagtc agaaggagaa ggtacacttg ctgtaatcat
    10021 acataaagag gaaggtgaag tattaccagt atttacagta ataggtgtaa tagcagaaaa
    10081 aggggaaaat caagaggaag taaaagctaa atatttatct ggaaatgttt caaaagaaga
    10141 tatagttgaa gagaatcaaa atatagaatt taaagaagaa aaaataaaca aaaaagagtg
    10201 taatcatgat tatgatgtag tggtaatagg aggaggacct gggggatatt tatcagcatt
    10261 aaaagcagct cttttaggtg gaagagttgc tttagttgaa gaaaatatac taggaggtac
    10321 ttgcttaaat agaggttgta tccctacaaa gacttatata aaaacagcag aaatattaga
    10381 agaaattgac caattatcaa agagaggtgt aaaagtaact gtagataaag aacaggacat
    10441 taagaaggcc ataaaatata aaaatagagt agttaaaaaa cttacagctg gtgttggcgg
    10501 attattaaaa agtagagatg tagatgtgtt taatcttaaa gctagtgtta aagaagagca
    10561 taaagtaatt ttatcagatg gaaaagtttt agatacagaa aatataataa ttgctacagg
    10621 ttccaaagta aggatattac ctataaaagg aatagaatca aatttaataa taactagtac
    10681 agaagcatta gatttggaaa cagtgccaga agaattagta ataattggtg gtggagtgat
    10741 tggttgtgaa tttgcagaga tattcaattc aagaggttct aaagtaacta tagtagaaat
    10801 ggaagataga gtaatcccaa gaatggataa agagttaagc gaatctttaa aatattctct
    10861 tagtaaaaaa ggtataaatg tattaactaa aaagaaagtt tctgaattta aagaagaagg
    10921 aaataacata ttagtgtgca ttgaaggtga agagccaata aaagcagatt tatgtctata
    10981 tgctatagga agagaagcta atctttctgg catagaggac ttagatatta aaatagataa
    11041 aggttcaata gttgtaaata gtaaaatgga aacaagtata ccaagcatat atgcagtagg
    11101 tgatgttact ggaggtgtaa tgttagctca tgctgctttt aaaatgggag aggtagcagc
    11161 ttcaaatgct ctaggtgtga ataaagaagt tgacttaggt gcattaccta gttgtgttta
    11221 tacaattcca gaagtagcat cagttggtat tacagaagag gatgctagaa aaaaatataa
    11281 tgtaaaagtt ggtaagttta attttgctgg aaatggaaga gctttagctt ctggtcaaga
    11341 acaaggatat gtgaaggtag tagcagatgc taaatatgga gaaattttag gtatacatat
    11401 gtttggatgt ggtgtagcag aacttataaa tcatgcagca tcatttaaag cgttagagat
    11461 acctactgat gaagcaagtg agttgatatt tggtcatcca tgtacttcag aagctttaat
    11521 ggaagctttg gcagatgtaa atggagaatg tttacactta cctaaaaaat aaatctaaac
    11581 ataaattatt aatttaagtc tattattaat attaatttat atcataaaat tttaaaacac
    11641 ttacttagcc atacccctta ttatgcaaat aatttatttg cataataagg ggtatttttt
    11701 atggaaaaat atagaatata gaatccttgt agtttttgtt gtacaaaaaa gatagtaata
    11761 taaaataaca ttatcatttt agaatgattt caattgtaat taggaaaagt gatttttttg
    11821 tttttaaaaa aagtattatt tgtaaaatat aaaaaaaaat ttcttaatat ttgaataaaa
    11881 gaatagctta atttaaatgt tattacttta taaaaaaata ataagtacaa aaaaaacaat
    11941 atatggcata taagttgcta tataaataat aaggagggaa caattataaa tgaggcaaag
    12001 aataaaaaac ttaattgaaa atgaagataa gaaaaatcct ttaacagatg aagcgatttc
    12061 aagtaggtta aatattcgaa gagaagatgt tacctttttg agaaatgagt taaaaataga
    12121 agattcaaga caaagaagaa aagttgtttt aattaaagct ataaaagata ttttaaaaga
    12181 agaaaaaact ataaataaaa atgatataac aagaagatta aacagcattg ggtttacagt
    12241 atcaagattt acagtcatac aatacttaaa agagattcaa gattctggtg acctaaaact
    12301 tgataaagag agtgatgata aaaaagaaga aataatcaat aatagagaaa ataaagatat
    12361 agcatttgat aaacttatag gtagtagtgg tagcttaagt acaattataa aactagccaa
    12421 agcagcaatt ctttatccac ctcatggtct tcatactatt attatggggc caactggtgt
    12481 tggaaagagt gagttagcag agtgtatgta taaatttgca gtagaatcat ccagattccc
    12541 taagaatgca ccatttatag tttttaatgc agcagattat tcagagaatc cacagctttt
    12601 gctatcacag ttatttggac acgttaaagg tgcttttaca ggagctgatg agaacaaaga
    12661 aggattagta tcaaaggcag atggaggaat attgtttata gatgaaatac atagattacc
    12721 tcatgaaggt caagaaatac tatttcaact gattgataaa ggtatggtca gaaagttagg
    12781 agaaacaaga ctaacacata aaatagatgt aatgattatt tcagcaactt ctgagccaat
    12841 agattctcat ttacttaata catttaaaag acgtatacct gtaacaattg aaataccaga
    12901 attagttgca agaccattaa atgagaggtt tgacattata aataatttct tcttaataga
    12961 agctcaaaga atgggagcaa acatacatat aaagtcagat gtattaaaag ctcttatgct
    13021 ttacgattgt ataggtaatg taggtcaact tagaagtgat attcaagtag cttgtgctag
    13081 gggactatta aaccaactta caaacaaact aaaagaagta aatataagca tatcagatgt
    13141 acctggatat gtaaaacaag gtcttataaa aatcagaaat tgtagaggga aaatagaaaa
    13201 ttatgtagat ggggatttaa ttatagactc aaatcttcaa ggtgagatag aaaagaaaaa
    13261 tgaagatata tatacttttc cagatgaaat atacaaactt actgaaagaa gacatattga
    13321 tttattggaa caaggtttag attttgatgt tataaataga atcattggag gagaattaga
    13381 aagttgcatt caaaaataca taaaacaagt taaaaaagtt tctattagta acaatcttcc
    13441 agatataagt ccaattgttg gaaaatatat cgtagagcta acttatgaag taatcaaaat
    13501 agcatcacaa tacctaagtt cactagaacc gaatctacat ttatgtttag cagttcacct
    13561 aaaagcagct tttgagagat taaaagaagg aaaagtaata tcaaatgctc atttgaaaga
    13621 aataatagga aattatccag tagaatattc tgtagcagaa aaggtagctc aatatttaaa
    13681 ggaaagatat aacataaact taccaaaaga agaaataggt ataatagcaa tatattttaa
    13741 aatgagtagt agaagagata tgtctgaaca aaaaaagata ggcgtacttc ttatggctca
    13801 cggaagagta gcatcttctg tatgtgaggt tacaaataaa ctattaggaa ttaatcacgc
    13861 aaggtattta gatatgccat ttgagaagaa gccggaagaa atgattgaag aagctactac
    13921 aataataaaa gatattgatg aaggtagagg tgtgattata ttagtagata tgggttcact
    13981 ttcatatttt ggagatatta tttctggaaa gactggaatt aatataagga caatatctag
    14041 atttgataca ttgctttcta tagaggttat acgaagagca gttttaccag atttaacaat
    14101 agacgagata gttgatgaag ttaaaggtat agagccacaa gtaaaagaaa aggttagtaa
    14161 atgtaacaat acaaaagtag attttagaaa acctataatt ttaacattat gtattactgg
    14221 attaggggga gcttgtaaga taaaaaaaat cttagaggaa aatattaaaa gtataactga
    14281 aaaggttgat atagaaccta taggtatgat tgaatatcca agtcttgatt tagagataaa
    14341 acaaataagc aatgataaaa atgtaatcgc catagttgga acaattgacc ctcagatagc
    14401 aaatatccct ttttttagtc tagtagacat aaatgataaa gcgaagttaa atcaaataaa
    14461 taacctaatt gatacaaatc aaattggtga aacaaataaa aaatatgaat tatcagattt
    14521 aatacataaa gaagatatat ttgtggattt acaggttaaa tcaaaagaag aagttctaaa
    14581 atatatgagt aaaaaactta gagagaaagg ttatgtgaca aaaaatttct ataaaaaagt
    14641 agtggaaaga gataacttat ttccaactga attatcagat gaaatagcaa tacctcacac
    14701 agatggaata gatgtaatta ggccagctat aagtatagcc atcttaaaga agcctattgt
    14761 gtggagtaaa agtaaagtaa aaataatact tttattagct gtagaccaaa agtgttttaa
    14821 tccattaagc actctactct catttataga aacagatgaa tttaagaaga taaaaaacat
    14881 taaagataga gattttatta gggagatgat attagatgga gtcaatgaaa ataattagta
    14941 gcaatttaat ttttaaaaat attgaagtgt ctaacaatga agatgcatta aagtttttgg
    15001 gacaacgttt gtttgacgaa cagtatgtaa aagaaagtta catacaagct gttgtagcaa
    15061 gagaaaaaaa gtatgctaca gggttaccaa cagaaatata cggagtggca ataccacata
    15121 cagatatagt acatgtaaat gagccaggaa tagcaattgg aatactgaat aaacctgtaa
    15181 aatttataat gatgggtaca gacgatactg aaattgatgt aaaagttgta tttatgttag
    15241 cagtgaaaga gccacaagaa caattgcaac tattagaaaa acttatgact atatttcaag
    15301 ataagaatat actaaataat attgttgact taagtgaaga aagtgtgagt gatttattaa
    15361 actctaaatt gaaaaattaa acatgtaaag tatattaaaa atgtaaaatt aaacatgcaa
    15421 agtatgttaa aaaatataaa aagattatta aagatttcta actatagata tctttaaaat
    15481 aaaataattt caatgggggt gactaatgtg gctaaaaaga tattagttgc atgtggaaca
    15541 ggagtatgta cttcaacaat tgctataaac aaattaaaga aagcacttca agacattggt
    15601 aaattggata tggttaatat tacacaatgt aaagtagtag aagttgcatc aaaagcacct
    15661 gattatgatt taataatttg tactacacaa gtatctagta gtataaaaac accagtgata
    15721 aatggattac cttttttaac tggtgtcgga atggataaat taatcaatga tgtattagaa
    15781 gagttagaat tataattaaa aaaattaagg aacatggtct ctaaaatcat aagttttaga
    15841 gacttaaaat taaataatgg agggtcttta tatggagata ttaaatttca ttgttggtct
    15901 aggtgcacaa gtaatgatgc caataataat atgtattttt ggtttgatat taggtacaaa
    15961 actaggaaaa tctttaagag ctggtttaac tgtgggagtt gggtttatag gtcttaacac
    16021 tattatagct ttgctaacag ataatttagg tccagctaca caacaaatgg ttaagaattt
    16081 agggctaagt ttatcaataa tagatgtagg atggccagca gcatcagcta tagcttttgc
    16141 atcaacagta ggggctttaa ttattccaat aggtcttgtg gtaaatatag ttatgctaat
    16201 tactaatact actcaaactg ttgatgtaga catatgggat tactggcatt ttgcttttac
    16261 aggagcttta gtggcaggtg ctactcaaag tgtaatgtgg ggagtatttg catcagtagc
    16321 aaatatggta attgtattgg taatggcaga tttaactgct ccaggtatag aggaatattt
    16381 aggtatgcca ggtatatctc ttcctcatgg atttacacag gcatttgttc caattgctat
    16441 agtagtaaat aaattattag acctaatacc aggtataaat aagattgaga taaatgctga
    16501 tacattgcag aaaaaatttg gattatttgg agaaccttta ataatggggt ctgtaatagg
    16561 tgtaataatt ggtattgctg cgaaatatga tataaaagga atattacaat taggtgtaac
    16621 tatgggagct gtattaatat taattccaaa gatggcagca ttattaatgg aaggattatt
    16681 accagtatca gaagctgcac aggaatttat tgagaagaga tttaaaaaca gaggcaagat
    16741 ttatataggc ttagattcag cagtaggaat aggtcatcct gttacattat cagttgcact
    16801 agtgttggtt cctttgacta tacttatagc agctatattg ccaggaaata aagttttacc
    16861 atttgcagat ttagcagtaa ttccatttgc acttgtatta atcgtaccaa ttacaaaggg
    16921 aaatgtgttt agaacattaa taataggaat aattataatt acttctggat tattaatcgc
    16981 aactaactta gctccattat ttacccaaat ggctttaaat gctagtttta aaatgcctga
    17041 aggagcaact atgatttcaa gtatttgtga tggtgcgaat ccattaagtt gggtatttgt
    17101 aaaagttatg aactataaag ttattggagg agtagttttt ggagttatag ctttaggaat
    17161 ggctatttac aataggaata gaataataaa tgaaaataaa aagctatcta tagaagaata
    17221 aggttaaaac aattttatta aggagtgtat aggttatgaa aaaatataaa tatgattcta
    17281 aaagatactt actcagtgtt acaataccag gattttttat gattttaata ttgatttatg
    17341 cactgtttaa taactataca aattttaatt tgaatatata tagtttgtta ataattgtct
    17401 gtacttatgg tatattcaat acttttatat catcaagtaa tcctaaaaaa attattattg
    17461 atagcaaatg tatacatttt acctcattta tgactacaca taagtatgaa attaaagata
    17521 tagaaaagat aagaataaga gaattttata ataaaaaaat ttatgtaaaa attaatgatg
    17581 gaaacctttt taaaggtagg tattggataa gaacaaatat gtttaataat agtatagcat
    17641 tatatagcta tttcattgag ttggaagagt gcttatatcc agattcacta aaatttagaa
    17701 ataaaaaatt tgagaataag aacatataaa aataaaaaat tgaattgaag cctagcttaa
    17761 tcttttttga taagatataa gtttgaactt tatactaaat ttttagaatt ttcaatcaag
    17821 acaataacat aatagctaag caattagaac acaattaaga tgtataaggc atgtatatga
    17881 tgaatgggag gatttaatta atgttattag agaaattaag aaaagaagta ttacaagcat
    17941 ctttagacct tttaaactat aatttagtga cattaacagg aggaaatgta agtgggagag
    18001 atgaacaaac tggctatata gcaatcaccc caagtggaat ggattataga aatcttacac
    18061 caagtgatat tgttatagta gatgtagatg gaaatattat agatggaaaa tggaaagcat
    18121 cagtggattt atcagaccac ttgtatatat ataagcatag agaagacata aatagtataa
    18181 ttcatacaca ttctacatac tcaagttgtt ttgctatttt aaatgaacct atagaatgtg
    18241 caagtacaac tcttgcaaat gaagtagggg gttcagtacc agttgcaaaa ttttcaccac
    18301 caacatctaa aaagatggga aaatgtgtta ttgaagcaat tggtgacaaa agagcatgtc
    18361 ttttagcaaa tcatggcgta attgcagttg gtccatctgt aggacatgct cttacagccg
    18421 ctgtaatgtt agaagattca gcaaaagtat attacttagc aaaatcaatt ggcacaccag
    18481 tgctactgcc tgatgaagaa attcaaagag cgagagatgt attttttaat gtctatggtc
    18541 aggataaata aattagataa aattttaaaa gtaatatgaa aatatagata ttcaatgata
    18601 ttaaggagat ttataatgat ataataagtt taatagttaa gaagtaaaat atgaaatgta
    18661 atagtatata aattcattta agactaaaaa gaataagata aaataaaaag gagtctgtat
    18721 ttctatgtat tacttattaa acaaaagcac aaatccttac tttaatttgg cattagaaga
    18781 atatctattt ttaaatgata aatataatga tgatattata atcatatgga gaaatgaaga
    18841 atccattttt ataggtaaaa atcaaaatcc atatcaggaa gtatatcatg atgttattga
    18901 aaagggagaa attcctattt taagaagaat atctggagga ggaactgttt atcatgactt
    18961 aggtaatatc aatatgtcat tcatacaaaa agataggcaa cttcatgaga tagatttttt
    19021 ggagcatact aagtttatgc aaaagatgct ttcaacctta ggactagatg tatctataac
    19081 tgaaagaaaa gatttatttt taaatgggaa aaaaatttca ggctctgcac agtctataaa
    19141 aagaaaaaat tctctttatc atggaactct tctttacgat tcagatttaa ataaattaac
    19201 taaatattta aattcaaata aagcaactga atcaaatgca actaaatctg tttcaagtaa
    19261 agttacaaat attaagtttt tactcgaaaa agatataaat tactttttag attattgtgt
    19321 tgaatattta aaatctaata taaagaatat aaaagaactt gaattggatg aagaagatat
    19381 taaaagtgta tacaatatag gagaagataa atataaaaag atagagtgga catatggtaa
    19441 aacgccgaaa tttcaagtag tgctaccatt taataatgaa agtaaaataa atatacatgt
    19501 taacagatgg aaagtatcaa aattttttat ttcttatgat gataatgtaa ttaatatgga
    19561 caagttgtta gatttgaatt tctttaaaga agaaataaaa gagagtattt tagaaaatta
    19621 tccacattac atagatttaa tcaatttaat attttaatat aaaacctcta atttgataaa
    19681 attgtcttat atcaaactag aggtttttaa tattcttgca tattaacaac cattacaaaa
    19741 ttgtaaaatt aaaaacaaca ctattgaaaa atcaatatat attgtaataa atatatgtgt
    19801 ttttcattag caaatagaca ttaactattt atacaactga aaatttaata tataattaat
    19861 cagatggtta aaataaaaat attaaaagtt ttaataaatg atggttaaaa ataaaatacg
    19921 aggtgatacc atgtatagtg ttataattgt tgcagcggga agcggaagga gaatgaactt
    19981 agatataaac aaacagttca taaaattaag agaaaaagaa attattgctc atacaattca
    20041 agttttttat gaaaatataa atatagatga aatagtggta tgtataaaaa aagaagaaga
    20101 agattttttt aaagaaaata taataaataa gtataacttt aaaaatataa aaatagcata
    20161 tggcggaaaa gaaagacagg actcaatata taatggttta aaaaaattag atgaaaattg
    20221 tgatatagta cttatccatg atggagcaag accatttgtc gaccatagaa taatcaatga
    20281 atctataaag gtagctaaag aaaaaaaggc tgtggttgta ggtgttccag taagtgatac
    20341 tataaagata gtgtcagatg gaacagtaca agaaactcca gaaagaaatt tgctgtgggc
    20401 agcacaaacc cctcaaacct ttgaatataa tctcataata gatgcttatg aacaagcata
    20461 taagaacaat tattatggta ctgatgattc aatgttggta gaaaatatag gtcaaagtgt
    20521 cactatggta atgggttctt atgaaaacat aaaaataact agtccagaag atttaaatat
    20581 agcagaacaa atattaaata tggaaaaaag agatgaagtt aacagtagaa ggagaattat
    20641 atgagaatag gattaggata tgatgtacac aaattaacag aggatagaaa acttataatc
    20701 ggtggtgtag agataccaca cgataaaggt ttgttgggtc attctgatgc tgatgtattg
    20761 atacatgcaa taatggattc tattttaggt gctctagcat taggagatat agggaagcat
    20821 ttcccagata cagatgaaga atataaggga gcagatagta tgaaattatt agaacatgtg
    20881 tataatctaa taacaagtaa aggatataaa attggtaata tagacagtac tataattgct
    20941 caaagcccta agatggctcc atatattgag agtatgagaa gtaatatatc aaaagtttta
    21001 aacacagaca ttgataatat aaacataaag gcaacaacag aagaaggtct tggttttaca
    21061 ggagcaaaac agggaatagc ttcacaaagt atttgtttat tattgttgac aagtcagaat
    21121 aattaatata ttattagtaa taagtttaat ggatatcaag taataggcct atatagaata
    21181 tctataatgt atgaatttaa gtgaaattaa tataaatata aaattagcta tgataaagaa
    21241 atagtaaaat gatgaaatct tcacagagag aaaacggtgg tgagagtttt caagaggaag
    21301 tctttgaacc tgtcttttag cttaaaggta taaaaatctt tacggtagga accgttatat
    21361 tcccaaaaga gagctattca tatagctaac tagagtggta ccgcggaaat taaacctttc
    21421 gtctctatac ttgtatagag atgagaggtt ttttatattt tcaggaagtt aatattcagg
    21481 aggaatctat taatgaaaat gtcaaaaatg tttatgccga cattaaaaga aatcccagca
    21541 gatgctgaga ttacaagcca tcaattaatg gttaggtcag gcatgataaa aaaaatgact
    21601 tcaggtgttt ataaccaatt accaatgggg ttaagagtgt tcaaaaaaat tgaacaaata
    21661 attagagaag aattaaataa aaaagattgt caagaaatac tttgtgcagc cttacttcct
    21721 tctgaattat ggaaagagtc tggaaggtgg actgctatgg gtgaagaaat gtttagacta
    21781 aaagacagaa cagaaagaga gtactgtctt gggccaacac atgaagaagc attcactgat
    21841 ataataagac aagaaattac ttcatataaa caattgccac taaacttgta ccaaatacaa
    21901 gtaaaatata gagacgagag aagaccaaga tttggtgtta tgagaactaa gacttttaca
    21961 atgaaggatg catatagttt tgatgtagat gataaaggtt tagataagtc atatcaagat
    22021 atgtttgatg catatgtaag tatatttgat agatgtggat tggagaatag ccctgtacag
    22081 gcagattcag gagctatagg aggttctaca tcagccgaat ttatggttaa gtcagaagtt
    22141 ggtgaagatg aagttgtatt ttgtagtgga tgtgattatg cagccaatgt agaaagagca
    22201 gaatcttgta atctagcatc acaaaaagaa gaaatgaaag aattagaaga ggttcataca
    22261 ccaggagcag ctacaataaa agagttagaa gaatttttaa aaacttctcc agataagttt
    22321 gctaaaactt tagtatatga ggcagatgga aaaacagttg tagtcgttgt aagaggagat
    22381 agagaagtta atgaaatcaa agtatctaat gccataggtt ctgttataga atttgctctt
    22441 gctacagatg atgtagtgag aaaagtaact aatgcagaag taggatttgc aggtccaata
    22501 ggtataaatg ctgactatgt atttatagat aaagaaattg ttgaacaaag aaacatagtt
    22561 gttggggcta ataaaactga gtaccatata aaaaatgcta actatggaag agattttgaa
    22621 ggaatagttg gagattttag aaatgttcaa gaaggcgaca agtgtatagt atgtggtaag
    22681 ccacttgaaa tagctagagg tgttgaagta ggacatatat ttaaactagg aactaagtac
    22741 tcagaatcta tgaatgcaaa cttcatagac aaagatggaa aatcaaagcc tatagtaatg
    22801 ggatgttatg gaataggtgt tgaaagaaca gcagcagcta taattgaaca acataatgat
    22861 gagaaaggta taatatggcc tttgtcagta gctccatacc atgttgtgat agttccagca
    22921 aatatgaaaa atgaagaaca aatatcaata gctgaaaata tatataatga tttgcaagct
    22981 atgggtgtag aagttttatt agatgacaga gatgaaagaa taggtgtcaa attcaatgat
    23041 tcagaattaa taggaatacc tatgaggatt acagtaggta aaaatattaa tgaaggtaaa
    23101 gtagaattta aacttagaca taaagaagat aaagagataa ttgacataga agaaataaat
    23161 gaaaaagtaa aggcagagtt tatcaggaat aatgttaggt taggataata acttaaataa
    23221 gatattgtat agataatcaa agttgtactt tatagacacc attttatcta tatataagga
    23281 tataaaatgg tgttttttta taaaacaaat aaatgaggag gtaattaaaa tggcaaaaaa
    23341 tgaaaaacaa tttgttgaag aaataacaaa gatggaagat gattttccac aatggtacac
    23401 agatgtaata actaagactg acttagttga ttatgcacca gtaaaaggtt ttatggttgt
    23461 aaagccatat ggatatgcat tatgggaaaa gatgcaagaa tttatggata aaaaatttaa
    23521 agaaactgga cataaaaact gttattttcc attattaata ccagagagtt tattaaacaa
    23581 agaagcagaa cacgttgaag gttttgctcc agaagttgca tgggtaacac atggaggaaa
    23641 taaaaaatta gaagaaagat tatgtgtaag acctacttca gagactataa tatgtacaat
    23701 gtatgcgaaa tggttaaaat catatagaga attaccatat ctttataatc aatggtgctc
    23761 agttgttaga tgggaaaaat caactagacc tttcttaaga acatctgagt ttttatggca
    23821 agaaggtcat acgttacatg aaacagcaga agaagcacaa gaagaaacaa tacaacaatt
    23881 agaagtatat aaagcgctat gtgaagaatt acttgcaatg ccagttgtag ctggtcaaaa
    23941 aagtgaaagt gaaaaatttg caggtggtga aagaacatat actatagaag ctatgatgca
    24001 tgatggtaaa gcattacaat caggtacaag tcacttccta ggtcaacact ttactaaggc
    24061 ttttgatatt acttttgcag atagagaagg aaatttagct aatccatatc atacatcttg
    24121 gggagcatct acaagactta taggtggact tataatgact catagtgata acagaggttt
    24181 agtattacca ccaagagtag ctccaattca agttgttata gttcctatag ctgctaaaaa
    24241 aggaaatgta atggagactg ttgataaaat atatgctgat ttaaaagcta agggagtagc
    24301 tgtagaagtt gatgatagag ataactatac aactggatgg aaatttaatg agtgggaaat
    24361 gaaaggtgtt ccagtaagag ttgaaattgg accaaaagat atagaaaata atcaagctat
    24421 ggtatttaga agagatactt tagaaaagga ttctatgcct ttagaaggat tagctgatgc
    24481 tatatgtgat ttatttgatg ttatacacaa tgatatgttt gaaaaagcaa gaaaacatag
    24541 agaagacaat acatctatag ttgaaaatat ggatgagttt agaaaagctt tagaagaaaa
    24601 gccaggattc ataaagacta tgtggtgtgg agatgcagaa tgtgaagcta agattaagga
    24661 agaaacagga gcaactataa gatgcttacc ttttgaacaa gaaaacttag gtcacaagtg
    24721 tgtgtactgt ggaaaagaag cagatagtat ggtagtaatg gcaaaagctt actaaaatac
    24781 atttaaataa aaactggggt agaaagtatt taatacttta tcccagtttt tttgtttttt
    24841 atgagtaata aaatgtattt gtaactatgt cataagataa gtcatagata cttatattaa
    24901 aaagaatata atagagaact atttttaata attacatatt aatgaaagca aaatattttt
    24961 gttatataga ttttcaataa agcaaacaat aaatataaat gaatattagt aaggagtgct
    25021 tttaattttt agaaaataaa tgacttaaaa gaaagattta tgaaattaaa actaggattt
    25081 tctgatataa taaacatgtg tatttttaaa taaaaaggat attattatta gatttgatta
    25141 aaaataaact tgcaaaacaa tgtactagtt taatattata gttatattga ataaaatatc
    25201 aggaggaagt aaaaatgaat gtaagagtaa ggtttgcacc aagtccaaca ggatttgtac
    25261 atataggtag tttgagaact gctttatata actatttatt tgcaaaaaaa atgggcggag
    25321 aatatatatt aagagtggaa gatacagacc aaagtagatt agtagaaggt gctatagaga
    25381 atatgcttaa tgctatgaaa tgggctgggg tcaaccatag tgaaggtgtt atattagatg
    25441 atagtggaaa agtagtacaa aaaggagaat atggtccata tattcaatct caaagattag
    25501 atatatacca agaatatata aagcaacttt tagatagtgg aaaagcttat tactgtttct
    25561 gtacaaaaga aagactggat gaagtaagag atgctcaaag agcagcagga gaaacagcta
    25621 aatatgatgg tcattgtaag aatctttcta aagaagaagt agaagcgaat ataaaagcag
    25681 gaataccata tgttataaga ttaagattgc cagagaatca tacaattaag tttacagact
    25741 tagttagagg agatatggaa tttaatacta atgacttaga tgaccaagta ttaatgaaaa
    25801 cagatggatt tccaacttat cattttgcag tagttgttga tgactatttg atgaaaatta
    25861 cacatgttat aagaggagaa gaatgggtat catctactcc aaaacatgtt tatttatatg
    25921 aagcttttgg atgggaagca cctgtatttg tgcatttacc aaatatactt aataaagaaa
    25981 agaaaaaatt aagtaaaaga caaggtgatg tagctgttga agactttaag aaaaaaggat
    26041 atttaccaga gggattagta aactatgtag cattagttgg ttggtctcct gaagataatc
    26101 aagaattgtt tacaatggag gaattagaaa aagccttctc tgtagaaaga gtctctaaaa
    26161 gtggtggagt atttgatact gaaaaattaa attgggttaa tcagcattat ataaaagatg
    26221 gtgatgatgc ttatctaaca gacttagcaa taccattttt aatagaagat ggatttataa
    26281 cagaagaaga agctactaat aaatatgatt tcttaaagag tatgatatca gtacttaaag
    26341 agaaattaca atatgtaaaa gaagttactg aacatgcaag tatatttttt ggagataaaa
    26401 tagaggtaga aactgaagag ggaagagagt tcttaagatt agaacatata cctactttaa
    26461 tagatgcttt gagagaaaaa atagaaaaaa cagaggtatt aaatgctgag tttgttcaag
    26521 ctatgcttaa agaaatacaa aaagagtaca aaataaaagg gaaaaattta tttatgggct
    26581 caagaataat tttgacaggg caaatgcatg gtccagattt accaaaagta atggaagttt
    26641 taggaaaaga aacttgctta aatagaatag cttatgtaaa aaataatata ttatagtcat
    26701 aaaagtccgc taatttagaa gcggactttt tttgtgttag tatttggtga aaatatctgc
    26761 ttaagaaaat gaaggtattt aaggtaataa atttaatata taaaaagcaa agcaaaaata
    26821 taaataaaat atgaataata aactaataaa atattaagca tagaagaagg gaatctatta
    26881 ttttagtttg atttaattat taagggggta attactatgt atagtaatat tgatgatgtt
    26941 aaaaaggaat taaaggagct atgcttagaa tatgttacaa ttttagaaaa attaaaagat
    27001 gaaaaaatga ttacagaaga aacttttgaa aagtgctctt ctcaaaaaaa aatattttta
    27061 gaagaacaat agaggatggt aatagtatta ttattatgta gtttgaaaga tagtacgaaa
    27121 aatgatagta atactattat ttattgcaat tttcaactat aatataatat ataatgttaa
    27181 gaagataatt tataaaaata cagtgaggtg atgagtgtga aagtttataa tacattgact
    27241 agaacaaaag aagaatttgt tccattagaa gaaggaaagg taaaaatgta tgtgtgtggt
    27301 ccaacagttt ataactatat acacataggt aatgcaagac catttataat atttgatact
    27361 ctaagaaggt atttagagta tagaggatat gatgtaactt atgtacaaaa ctttacagat
    27421 gttgatgata agattataaa tagaagtcat gaggagggaa tatctcctga agaagttgcg
    27481 gctaagtata taaaagaata ttttgtagac tgtgatgggc taggaataaa aagagctact
    27541 gttcatcctc aggttacaga taatatacaa cagattatag agtttataaa agagttggaa
    27601 gataagggat atgcatatgc agtaaatggt gatgtatatt ttgatacaaa taagtttgaa
    27661 ggttatggaa aactttcagg tcaaaaacaa gaagatttag aggcaggggc tagaattgag
    27721 gttaatgacc aaaaaagaca tcctatggac tttgttcttt ggaaggcaaa aaaagaaggt
    27781 gagccaggat gggatagccc ttggggagaa ggtagaccag gatggcatat agaatgttct
    27841 gttatgtcta agagatattt aggtgaaaca attgatatac atgcaggtgg gcaagattta
    27901 acattcccac atcatgaaaa tgaaatagct caaagtgaag ctagaagtgg aaaaacattc
    27961 tcaaaatact ggatgcataa tggatatata aatataaatg atgaaaagat gagtaaatca
    28021 aaaggtaatt tctttacagt aagagatata tcaaaattat atgatttaga gatagtaaga
    28081 tttttcatgt tatcagcaca ttatagaaat ccagtaaact ttagtgatga gatgttaaat
    28141 caagctaaag caggtcttga aagactatat aatactaaag aaaaattaga atttacgcta
    28201 agtaatttag tagaatctcc tttaacagaa aaagaagtag agcttgttaa agaattagat
    28261 gactttagac aaaaattcat agatgctatg gatgatgatg ttaatacagc agacgcagta
    28321 agtgtaatat ttgaattagc taagttaata aattctaatg tagatgaaaa ttcttctttg
    28381 gagtttgcta aaaaatgctt agatgaattt aatgaactta caggagtatt aaatattgta
    28441 aataagaaaa aagatactgt attagataaa gacattgaag aattgataca aaaaagaact
    28501 gatgcaaaga aaaataaaga gtttcaatta gctgatgata ttagacaaca attactagat
    28561 atgggaattg tattagaaga cacaagacaa ggtgttaagt ggaaaaggat ataaaaatgg
    28621 aaaaaactga attagttact atgtcaccat tagttttagc atacttaggt gatacagtgt
    28681 atgagacata tataagagaa tatttaatta gacagaatac acaaagaaaa gttaatgatt
    28741 tgcataaact agctataaag tatgtaaaag ctaaagctca ggcaactata attcatgaaa
    28801 ttgaaattga gttaactgaa gaagagagta aaatttataa aaggggaaga aatcaaaagt
    28861 caaatacttc tcctaagaat gcagatatta tagactacaa acatgcaact gggtttgagg
    28921 cattagtggg atatttgtat ttaaataatg aaatagagag acttcaatat attatcaata
    28981 aaggaatcaa aattattgaa agagatatgt aaatattaaa gagtgttgtc ttaataataa
    29041 gtaataaggc aacacttttt ttataactaa tgtataataa atatatagaa attagtaatg
    29101 agaaaggata aaaagttatg aaagtaaaat taatatcaca tacacctgaa ccagaaaaag
    29161 taatagctat ggcagctaag ctatgttatt caccagttgg gacagacgaa atagaaaaag
    29221 acttaacaga tgaaagtata gagaaatttt taaatatgtt attaagtata ggtcatgggt
    29281 caatattaga acatgcttca tttacatttt caatagaagg tatttcaagg gcttgttcac
    29341 atcaaattgt tagacatcgt atagctagtt tttcacaaca aagtcaaaga tatgttaaat
    29401 tagaacaatt tgagtacata attcctccag aaatagaaaa aatagaaaag gcaaaagagt
    29461 tatttataga ctccatgaaa aaagaccaag agaattatga taagttagtt gaaatattgt
    29521 ttgaaaatca ttataatgac ctaataaaaa acggtaaaaa tgagaaaaca gctaaaagac
    29581 aagctgaaaa gaaagcaata gaagatgcta gatacgtatt tccaaatgca tgtgaaacaa
    29641 aaatggtatt tactataaat gctagaagtt tgtttaattt ctttgaacat agatgctgtg
    29701 aaagagcaca gtgggaaata agaaatttag ctgtggagat gttaagagag gttaaaaagg
    29761 ttgctcctat attatttaaa aagaccggtc caagttgtgt aaatggaagt tgtccagaag
    29821 gaactatgac ttgtggagat atagttcaag ttagagaaaa atttaaggcc ttgtaggagg
    29881 tgaataattt ggcgagtata gaaggaagaa atccggtaat agaagcaata aaaagcgaca
    29941 gagaaataga taaaatatta attgcaaatt cagctaaaga aggttcaatt aagaaaataa
    30001 taggaatggc taaagataag aatataatta ttcagtatgt tgataaacat aagctagatg
    30061 aagtaagtac aagtcattca catcaaggtg taatagctta tgcaagtgag tataagtatt
    30121 atgaattaga tgagctaata gatttagcaa agaataaaga tgaagaccca tttttcatta
    30181 tattagatga gataacagac ccacataatt tgggttcaat aataaggaca gcagatgcag
    30241 taggagcaca tggagttatc attccaaaaa gaaggtctgt acacataact cctgtagttg
    30301 ccaaagcatc tgctggagca gtagaatata tgccagtttg caaggtaacc aatatagtta
    30361 atacaataaa aagactcaaa gaagaagggc tgtggattgc tgcagctgat atggatggag
    30421 aaacttttta taaacaaaat cttactggtc ctcttggtgt agtaattgga agtgaaggtt
    30481 ttggaatatc tagattggtt aaacaaaact gtgactttat agtaaaaatg ccaatgatag
    30541 gtaatgttac atctttaaat gcatctgtag caggtggaat acttttatat gagatattca
    30601 gacaaagact ggataaaagt aaataggcat gaaaaaaaat ataaatcatt acttaataat
    30661 agatggctac aatattataa atgcgtggga caatttgaaa gaactggcta aagaggactt
    30721 agaagattct cgagaaaagc ttattgatga cattatagaa ttttctgagt ttatgggtta
    30781 taagactata atagtttttg atgcttataa tgttaaaaat tctcgagaaa aagttgaaaa
    30841 aagaaaacac ataacaatag tttatacaag agaacaccaa acagcagata gttatataga
    30901 gaaatttata acttctttat caaagtatga tgatgtaaaa gtagccacta atgactatgc
    30961 agaacagcaa atgattttag gaaaaggtgc tactagaatg tctgcaagag aattaaagtt
    31021 agagttagat cgttccaaga ataaaatgaa ggaaaaaaat atcggtttga ggaaaaaaat
    31081 tcagcgaaat tggttggagg aaaggttaga taaagaaaca ttgtcgaaac ttgagaacat
    31141 tcgtagaaag cgttgaaatt actaggttct ttagactata atatacctat aaatttgttt
    31201 tatatatggg ggagatttac atgttggtag caaaagaaaa aagttatgag ttagtggaca
    31261 attgtcagca ggatgagtat aacattgtat taagagcaag tgaaggggat aaaatagcac
    31321 tagagtacat tattactaaa tacagaaatt ttgtaaaggc aaaagcaaag tcatattttt
    31381 taattggagc agataaagaa gatataatac aagaaggaat gataggcctt tataaggcag
    31441 ttagagattt tgatggaagt aagaccaatt catttaaatg ttttgcggag atatgtataa
    31501 caagacaaat aataactgct ataaaaactg caacaagaca aaaacatata cctttaaatt
    31561 catatgtttc tttgaataaa ccaatatatg atgaagagtc agacagaaca cttttagata
    31621 ttatagctac aagtatagta acagatccag aagagcttat aattagtaaa gaagagttaa
    31681 aaaatataga atcaaaaatg aacgaattgt taagtgactt ggaattagaa gtactagaat
    31741 tatatttaaa tggaaagtca taccagttta tagcggacaa gcttaaaaga gatgtaaagt
    31801 caatagataa cgctttacaa agggttaaga gaaaattaga aaaacatctt gaaaacagaa
    31861 atgattaata gtaaaatact taccataagc ttattgtgag caaaattaat ttatatcgaa
    31921 agataaggga ggaaattcaa aatggctaaa gctaaatacg aaagaacaaa acctcatgtt
    31981 aatataggga caataggaca cgtagaccac ggtaaaacta cattaacagc agcaataaca
    32041 aaaacattat atgacagata tcaattagga gaagcagtag atttcgcaaa catagataaa
    32101 gctccagaag aaagagaaag aggaatcaca atatcaacag cacacgttga gtatgaaaca
    32161 ccaaatagac actatgcaca cgttgactgc ccaggacatg ctgactacgt taagaacatg
    32221 ataacaggag cagcacaaat ggatggagca atattagttt gttcagcaac agatggacca
    32281 at
//
LOCUS       DAEPIN010000049        31485 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.049, whole genome shotgun sequence.
ACCESSION   DAEPIN010000049 DAEPIN010000000
VERSION     DAEPIN010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 31485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31485
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.049"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            complement(120..722)
                     /locus_tag="KR250_002435"
     CDS             complement(120..722)
                     /locus_tag="KR250_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 family protein"
                     /protein_id="HBH0068364.1"
                     /translation="MDNLIRILILAVIGGFIGYVTNVVAIRLIFRPIEPIKIPILNIE
                     IVGLIPKRRAEIAANVGEIIQEEFLSMDEILANIITDEDKEEVVRYIKARVKIIIHEK
                     VSFIPSGIKNMIQDYLGEIIESEVKQSIDELSKNIINKANERIDIQKMVEDKINELDL
                     YELEEIIIRIAKKELKHIEFLGLVLGFLIGIAQGLITMFI"
     gene            complement(902..1720)
                     /locus_tag="KR250_002436"
     CDS             complement(902..1720)
                     /locus_tag="KR250_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sporulation protein YtxC"
                     /protein_id="HBH0068365.1"
                     /translation="MVEKDNISSMKFLRNNNVNDRLIYTNDIVEVVIDSAKQEVLNKK
                     ILNGTKAEQRALNIDEISTEITSMIISIMKEKLLKEYILKCYGRTYPEDKNNIYIYSL
                     NIFAKKEIFMRETVFTRVRNYIEENDFINVEGFIRFRMREFMKYISAIGDIAVEEYLI
                     KKDQDEFIRVLKYFIDTQEEKIDLLKVHIMEDNTFVLYDKNGNKIDSIDDEEIINMVI
                     RENLNYEDFLISTLLTLCPKKIEILDLLNNNCSKEIIDTVEAIFENKVSIIMEN"
     gene            complement(1723..2484)
                     /locus_tag="KR250_002437"
     CDS             complement(1723..2484)
                     /locus_tag="KR250_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0068366.1"
                     /translation="MEFTREPFILDDDLCLALVDKRISYLMENELKLRNIEIIKTIEC
                     KELYESIKYHPDICICNLGKGDIIVAPNVYNQYKELLSRYKFNIIKGEANLLNRYPYD
                     IPYNIAIVGDYAIHNFKYTDKAILDYIENNKLKKINIEQGYSKCSICIVDRTSIITSD
                     KGIWKSMKNTEIECLLIEKGHINLFEMNYGFIGGCTGLISKDKLAFCGDVKKHPDYER
                     IKSFVESKNKEIVTLSCENLLDIGSIVPLMTRKER"
     gene            complement(2974..4086)
                     /locus_tag="KR250_002438"
     CDS             complement(2974..4086)
                     /locus_tag="KR250_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HBH0068367.1"
                     /translation="MKIKRILMACFVVVFTIFILAICNFDFGFDKVNKNNSNIDSAKS
                     KQSESKSLPTSAEKDKSNSKSKNKEDDTEDDSYVRKTIGVLLDTENLNNESYNNNVLS
                     ALKRADEDFDVRIKLEKPKTSKSFKESIEKLHKYEPDLIIAVGARFAEPIKEAATEYP
                     KQQFAIVDYTYKVQPANVISVSFQDNVTGYLAGLIAGKMTDTGKVGFIGGVEGSSRDK
                     YESGFRKGLKTSNKDAELLVKYSNIFENPESARTIAKEMRDSDRDVVFSATEDDSKRA
                     IQVARENGGKVIAINKDQYDMAPDNVISSIIKDVEQPVYELIKNVAKDGFKGSKVMEF
                     KIKDGELGLTSKSSRNIPPDVLEYIKKEYNKVKDTY"
     gene            complement(4212..5216)
                     /locus_tag="KR250_002439"
     CDS             complement(4212..5216)
                     /locus_tag="KR250_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HBH0068368.1"
                     /translation="MRFKRILILMLTVAMIAGMLVGCANNPSNNPSSSKDNKSDISDK
                     SNSKKSVSMILDIEGTNNEAMNNSALLALNNAQKKLNIDTNKVESDDSSTFSNSIDIL
                     CNDNYDLIIAVGARFAKPLEMVAKKYPKQQFAIIDYEYDKQPSNITSISYEDNKSGYL
                     AGLIAGKMTESDKVGFIGGTKSSSRDKFESGFREGVKFSNSSIKDISVEYADVFKDSK
                     SVESIAKKMMDNGVDIIFSTTEDDSKAVIDAVRAKNKKVIATNKDQHELAPENVISSI
                     VKDFENPTYNLIQSFVKGNYKGGKVIENTVKSGSTGLAQNSSQNIPPDVLEYVNKNNK
                     "
     gene            complement(5521..6369)
                     /locus_tag="KR250_002440"
     CDS             complement(5521..6369)
                     /locus_tag="KR250_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBH0068369.1"
                     /translation="MKNLKIICDSLSDVPKDLLDKYDIDIVSLTVILEEREYKDRIDI
                     SGDEFYKKLREENVYPKTSQATYAQFKEIFEKYTNEDREILYIAGSATATGTYQSAVM
                     AKNDVDGKIYTYDSNLLCMGTGLLVIKAGELASEGKSVAEILPVLDELKEKAFVIFSV
                     DTLEYLQKGGRISSTKAAIGSILNIKPILEIRDGLVSQISQVRGKKNVISKMMELIKS
                     NCGDDLSDQIVYIGYSDDFKEKEQLSKVMQDELRPKEIRFFQIGVCIGAHAGPGVTGI
                     ICLKNK"
     gene            complement(6405..6875)
                     /gene="trmL"
                     /locus_tag="KR250_002441"
     CDS             complement(6405..6875)
                     /gene="trmL"
                     /locus_tag="KR250_002441"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HBH0068370.1"
                     /translation="MSLNIVLVEPEIPQNTGNIIRTCAATGSTLHLVKPLGFSLDDKH
                     LKRCGLDYWDIADIQYYDSFEELQEKYPNSKYFFSTTKAKQSHSDVKYEENCFIVFGK
                     ETKGLPEPLLKANMDTCIRVPMLDVEKARSLNLSNSVAIVVYEALRQIGYPNMR"
     gene            complement(7069..8286)
                     /gene="nth"
                     /locus_tag="KR250_002442"
     CDS             complement(7069..8286)
                     /gene="nth"
                     /locus_tag="KR250_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HBH0068371.1"
                     /translation="MKNVNLKEINEKDVILEEEINKKGMDKKNIIEKEEINKKGIDKK
                     DIIEKEEINKKGIDKKDIIEKKEINKKGIDKKDIIEKKEINKKEMDKKDIIEKEEINK
                     KGMNKKDIIEKEEINEKEIDKMIGKKDEIIDEKDIENFMDIEEDKKSIKVSKKSTKKG
                     ETEKKTKKSSSNKATQKTSKETKSKFKIKNESANEKDVNKILDELEKLYPDAKCELNY
                     GTAFELLIATILSAQCTDVRVNKVTSELFKKYNTARDFANLSIEEISKEIKSCGIYKS
                     KSQKIKDTSEQLCELYDGEVPDSLEKLIKLPGVGRKTAGVVLSNAFNHPAIAVDTHVF
                     RVSNRIGIVDEPNPQKTEFALMEAIPKERWSHSHHVLIFHGRRMCKARNPECASCPIK
                     EDCNYYKELNETK"
     gene            complement(8484..10721)
                     /locus_tag="KR250_002443"
     CDS             complement(8484..10721)
                     /locus_tag="KR250_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBH0068372.1"
                     /translation="MKYNNTSEINPLDYILGQERAVEAMELGLKINNPAYNIYIAGEP
                     GTGKSTYALKVLNEYASKKNTHKDWCYIYNFENPREPIIVELEKGFGRELKKDMEKLI
                     ESLLEDFKNAFESENFEIEKNKLLDEYEIEKDMLLKQIKKYGEEKGFKLKQSKMGIVF
                     IPLKEEEDESDESDEEFYKAKRELENMAIQVVYKIRNLEEIAEKAVLELEEEIAKLVV
                     EPHIKRLCEKYENYDKIQTYLENIKKDIIEYMYLFYLDEEELKDKYDKEHFIKYKINL
                     FVDNGSSKNKESAPVVVEINPSPANLFGKAEYDYANGNIKTDFTKLLSGAFHRANGGY
                     LVLYADQLLKYTMSWEILKKTLQTKKVILETQTAIKPENMPLDVKLVLIGSHYIYDIL
                     YRYDEDFEKYFKVFVDFDSEMDKNEDNEEGIARFIAYQCDKNNLRHFTKSAVEEVIKF
                     STRLSGDLEKLSTKFNKIMEVVIEGSAYAEFRNSEYTKQCDVKKAISEKRKRINRVET
                     HMDESIENGFTLIETEGRKVGVINGLSVLSTGEYSFGRASRITATTSPGSKGIVNIER
                     EVNMSGSIHNKGVLILGGYLSENFAQDLLLSLNAYVCFEQNYGGIDGDSASSAELYVL
                     LSSLSGVSIKQNIAVTGSINQKGDIQVVGGISEKIEGFYSICKTKGLNGEQGVIIPRK
                     NQRNLVLSDEVNDAVRDGKFKIYTVERVEEAIEILTDVKFEEIKKLVKEKLISFSKIQ
                     TVSKE"
     gene            complement(10827..11741)
                     /gene="queG"
                     /locus_tag="KR250_002444"
     CDS             complement(10827..11741)
                     /gene="queG"
                     /locus_tag="KR250_002444"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HBH0068373.1"
                     /translation="MDNKELKAFCNLIGLNCVGVAGVDKYDNLEKILKNRQQKGYLTG
                     MEEPIIESRINPRHIMKDANSIIVCAFPYHINRQEKNKNSNLSKYCHGKDYHIVVKDF
                     LQQICDYISKTVTDFKYKLFADNGPLVDRYLAYLSGIGYFGINNNIITDEYGSYVFIG
                     YIVNNYKFEEDKPLEKSCFKCGKCVKYCPGNAILGNYDMNPKKCLSYITQKKGDLSEK
                     EKVVLKNGKKVFGCDICQEVCPHNVEIPTTHILEFKENIIDYLDYDEIQNISNKEFKR
                     RYGDRAFSWRGRNIIKRNMEIILDKYDD"
     gene            12582..12986
                     /locus_tag="KR250_002445"
     CDS             12582..12986
                     /locus_tag="KR250_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HBH0068374.1"
                     /translation="MIKITIPGRPISKSNFKLHNINGQAWMPSKGKYSKYLAYENMIA
                     GFINQQYQGETVEENLITVLKLFFPNKRMGDLHNYPKSICDGIEKSGIIKNDKQLKPV
                     LLFDFIDKDNPRVEIELYPVSKYDISYNIFEK"
     gene            complement(13243..14238)
                     /locus_tag="KR250_002446"
     CDS             complement(13243..14238)
                     /locus_tag="KR250_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBH0068375.1"
                     /translation="MRNLGNTNMKIKRVGFGGIPIQRITQDDTNLVINELEKQGINFI
                     DSARGYTISEEAIGIAIEGKRDKFFLATKSMSRDYDSMKRDVEISLNNFKTDFIDLYQ
                     FHNVKEEEYDNLFKDKMAYSALLEAKEQGKIKHIGITSHNLNTIEKAIEDGKFDTIQF
                     PYNIVEGQADEVFKKAHEKGIGIIVMKPLAGGALDNATLAIKYILSKDYIDVVIPGME
                     SVEQVRQNVAVLENLVLDEKDNKEIEEIRNSLGKKFCRRCEYCMPCAVGINIPLSFLC
                     EGYYTRYGLKEWAKEKYEVMDVKPTECIDCGLCESRCPYELPIREMLKTVVEKLG"
     gene            complement(14522..15358)
                     /locus_tag="KR250_002447"
     CDS             complement(14522..15358)
                     /locus_tag="KR250_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HBH0068376.1"
                     /translation="MIKLGTHLSIAKGFANAAETAVYIGANTFQFFSRNPRGGNAKEF
                     DEKDIIKFQEIRKENNFAPLLAHAPYTMNLGGTKDDVYEFATRVIDEDIRRMDSLGIE
                     YMCFHPGSHVGGGVDFGIDRIVKGLNNAIKGDENITILLETMSGKGTEIGFKFEQLKS
                     IIDKIEHKEKIGVCLDTCHIFSAGYDIVNDLDGVLEEFDKVIGIERLKTVHLNDSMMP
                     FGDKKDRHAPIGEGKIGLKALIDFMEHPSLKHLPFFLETPFDDEGHKKELKMIKEILS
                     SK"
     gene            complement(15846..16409)
                     /locus_tag="KR250_002448"
     CDS             complement(15846..16409)
                     /locus_tag="KR250_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0068377.1"
                     /translation="MKEVLVLRDLECIKAIAHPRRIDILKAFDKSPLSAKQLSQLLEE
                     PHAKINYHIKMLYKVGILELVEEKIKSGIVEKYYYPSAKNVVIGNRILNFSLDNGEEK
                     EELYISKFENMSEVFYKAAEEDVLENENIVDYHDISLTHDELVELSDTMKSKIDEILN
                     KRQHNVEGSKYDIAMVIVPTLEEECPS"
     gene            complement(16507..17436)
                     /gene="glsA"
                     /locus_tag="KR250_002449"
     CDS             complement(16507..17436)
                     /gene="glsA"
                     /locus_tag="KR250_002449"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HBH0068378.1"
                     /translation="MNTLDETLLKEIISSNKKYTNYGQVASYIPELKNARRNDLGICI
                     IDSENNLYSAGNCSTKFTIQSISKPIVLAMALMDNDWEDVFSNVGMEPSGDPFNSIMK
                     LEINDTKKPCNPMINAGAIVTTSLINGSCLEEKEERMLSFFRKLAKNDNIGINYDVYK
                     SEKMTGDRNRAMAYLLKSDGFIRGNVEDVLDLYFKQCSIEIDSVDLARIGINLANYGV
                     DIENGEHLMSEMVSRIVKTFMMTCGMYDASGEFAIKVGIPAKSGVGGGIMASVPGRMG
                     IGVYGPALDKKGNSVAGVKVLEELSNKLKLNIF"
     gene            complement(18001..19668)
                     /locus_tag="KR250_002450"
     CDS             complement(18001..19668)
                     /locus_tag="KR250_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HBH0068379.1"
                     /translation="MKKINLKIEGKDKEYSLEENSPGIRLGDIAKEFCDEHKGYITLA
                     VVDNKLKELNCRVKKDCEINFLDTTNEDGERVYFRVMSFIFVMACREIFWDSRVTIEH
                     SLSDGLYCEVHIDRKLKEADVENIKNKMKEIVNNDYVIEKIEVTRNEAISIFENNEMY
                     EKAELLKYKEYDSAKVYKCRNYIDHFYGYMLPSTGYIKSFDIKLYNGGVIILGPSEED
                     KTLPMKFVPQPKLSSVYYEAEEWSRLMGIDKVVSLNKIIENNEYGDIIRTFEALHEKK
                     LSQIADMIKDGNKRVVLIAAPSSSGKTSFAHRLSIHLRVNNLNPISISLDDYFIDRKH
                     TPLDEYGNYDFESIYAIDLEKFNLDLKKLLAGEEIDDIRFNFKEGKREYTGKKIKIDS
                     NQPIILEGIHGLNPILTSSIPDEDKFKIYISALTQINLDDHNRIPTADLRMIRRIVRD
                     YNFRGYSADNTILQWASVRRGEKKNIFPYQEEADAIFNSACVYELAVLKRFAKPLLEE
                     IKEDNPAHIEATRLLKFLQYFVELDDTSDIPGISILREFIGGSKIVD"
     gene            complement(19742..20497)
                     /locus_tag="KR250_002451"
     CDS             complement(19742..20497)
                     /locus_tag="KR250_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HBH0068380.1"
                     /translation="MAVGIASLLFNIEEALNICESIKQITHLEIGIDNISECSELCKY
                     KERISKLGLSIGIHLPMELNTCENIEYIRNSWINFIEKIEFELKGFDLRYFNLHLGYV
                     MTNRVRKNRDKYLGNSVDFLDKLNTNSYVCIENTYSKGGDFSNIGNISYDFEYIFKRI
                     KNSKICFCYDTGHCLIDEDAYVKNLKDKIRLIHLSDNDGINDTHVGIGRGILSEEGIK
                     EVLTLDAEYLVLEINYEDIEDTISKLNNIVGEG"
     gene            complement(20507..21043)
                     /gene="lepB"
                     /locus_tag="KR250_002452"
     CDS             complement(20507..21043)
                     /gene="lepB"
                     /locus_tag="KR250_002452"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBH0068381.1"
                     /translation="MNETIKEEIVEWIKIIITALFFAFIITRFIKPTLVNGESMYPTL
                     KSHDYLVANRMTYKLSEPKCGDIMIFKTDLLQENGRKKELVKRVIGVPGDHLKIKDSK
                     VYINGKLLNEVSYIHDNYTEGDIDMVIPKGKVFAMGDNREVSLDSRYKEVGLVDEENI
                     KGKVILRVFPFTDIGIFE"
     gene            complement(21209..21745)
                     /gene="lepB"
                     /locus_tag="KR250_002453"
     CDS             complement(21209..21745)
                     /gene="lepB"
                     /locus_tag="KR250_002453"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBH0068382.1"
                     /translation="MGEAVKKEVVEWIKVIVIALVLAFAITRFIVPTIVKGESMYPTL
                     VERDYLIVNRIAYKVGEPKYKDIIVFKTDLTEENGKKKDLVKRVIGVPGDHVKIQDSK
                     VYVNDKLLDETSYIHNNRTDGDIDIVVPEGKLFAMGDNREKSLDSRYDEVGLVDEHTI
                     LGKVLVRLYPFSKIGTID"
     gene            complement(21845..23155)
                     /locus_tag="KR250_002454"
     CDS             complement(21845..23155)
                     /locus_tag="KR250_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HBH0068383.1"
                     /translation="MTELPNEFLEDIEEILKDEYDDFIKSYEESKTTGLRINTLKIDK
                     KKLFDLELFDLNQIPWAEEGFYYDDKIDRPGRNPLHEAGAYYLQEPSAMSVIPKADIK
                     EGEKVLDMCAAPGGKSTYILSKLNDTGLLVSNEINASRIKALGENLERFGARNCIITN
                     TDSNSLRKTFNGYFDKVIIDAPCSGQGMFRKDEVAISDWSYAKVVECQSIQKEIIRDG
                     YKMLKKDGILIYSTCTFSREENEHVINEFISEYPHAELIEMERLWPHKIKGEGHFVAK
                     IKKLEEEECNVKELKIKNLDKEIKDYREFEKKFLKVDFYSNKKNKFYLRGENLYLLPE
                     IYPDTKKLKVLRYGLHLGVLKKNRFEPSHALSHYLKVEDVKNVENFSVQSESILKYLK
                     GDVVNSNDSRGWVLVSVEGIPLGWGKESSGVIKNHYPKGLRKVF"
     gene            complement(23234..23950)
                     /locus_tag="KR250_002455"
     CDS             complement(23234..23950)
                     /locus_tag="KR250_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HBH0068384.1"
                     /translation="MKLDLFLLAIVPILIGMFWIRSKDRYCREPLIHLIKFFLIGAFL
                     SVIIILLENLLMKFNVFEGYSELIYVSFVVAGLVEEGVKALILIPALIKEKHFTEKLD
                     GIIYSVFLALGFATIENMVYIFSESRNLALQVGINRAVISIPAHVMFAITMGYYISKY
                     KFEGNKNKRREYLFMAVLIPILLHGVFDFILMIEYRWAIILLIVYVIILWKINLDKLE
                     KYMNHSKKVFFGNLRKKKKK"
     gene            24195..25466
                     /gene="sleC"
                     /locus_tag="KR250_002456"
     CDS             24195..25466
                     /gene="sleC"
                     /locus_tag="KR250_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex-lytic protein SleC"
                     /protein_id="HBH0068385.1"
                     /translation="MQDGFLTVSIIDATNNRPIQNAVVNIYSMSNGSQSSSTLYQNLR
                     SNESGQVTGLVLPAPDVDYSLQPSDVRPYSQYIVEAIADGYETVVIEGTQLLATIEAR
                     QGVPMSPRTRSKRSFSRQSELIFDIGEHTLYGTYPPKIPESNLKPLPPPTGFVVLDNP
                     VVPEFIVVHDGLPEDSSAPNYWIPFKEYIKNIASSEIYSTWPEQTIYANVIAIISFTL
                     NRVFTEWYRNKGYNFTITSTTAYDHKFINNRNLFEPINVVVDAIFNTFIKRPPTSRQP
                     LLAQYCDGQKSQCPDQMTQWGSKDLGDQGYDYESILRYFYGDEIVFERAPIVSGVPVS
                     FPGTTLQVGSSGQYVRTIQNQLNAISNSYPAVPKVIEDGIYGTDTENAVKIFQGIFGL
                     PQSGVVDFKTWYEISRVYVATTRIASLNPLI"
     gene            complement(25974..26630)
                     /locus_tag="KR250_002457"
     CDS             complement(25974..26630)
                     /locus_tag="KR250_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0068386.1"
                     /translation="MKKGDFIWSGVLICIIAVLAIPSSREVFMEATGAHPYIGGFLKF
                     AVLATMGDLLGARILYKDWKIPVGVFYRAIVWGIIGVVTTYGMSIYSSGVGQAQVDGM
                     LPFEGIAFANALFKSATMNILMSPTVFLFHKVMDCFIDKKYEVGKGIKVKLKDVVNIV
                     DWNAYLGFSVLKTIPFFWIPCHTIVFLFPAEYRVIASAFASIALGLILALANKSKVAV
                     "
     gene            complement(27902..28312)
                     /locus_tag="KR250_002458"
     CDS             complement(27902..28312)
                     /locus_tag="KR250_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0068387.1"
                     /translation="MKIKKSLISAMMVSAMVLGGTGAVFANGNDSSNIALPKLESLIK
                     SGITDPTELEKYGVEHYTYEEYAKHIQELKDYAKDPNAVKDVSQKDLEETIKKMEQEL
                     EKIKTEGLKIMKPITIENEDGSQISIAYNYAAID"
     gene            complement(28528..29910)
                     /locus_tag="KR250_002459"
     CDS             complement(28528..29910)
                     /locus_tag="KR250_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HBH0068388.1"
                     /translation="MFIKLLEMSFTSGVLLLIVIFIRACLFKKLPKKVFVLLWLIVLF
                     KLLIPVSLNSGFSIYSIIESTFDVELGNTSTENYTLKKASDSTGKNNSDEFEIMSNIE
                     NIMMLEMLGILSFIVTIMFSILLCFRYFKIINLFKTSIHIKNHEFIKKWMCKNSRFRR
                     FIVCISEFTRTPLTSGILKPRIILPKQMNYANNQTLDFVLTHEMVHINSFDNLLKLFM
                     LVALVVHWFNPLVWVMFFLLNKDIEFSCDEKVMSILGEEYRADYATTLISLAEENNPF
                     SMTICSGFGKTKIEKRIVNIMKFKKSTYVTFLVSTVLVCGTGLVFATENKEINEVESF
                     GLTKNEQSDKIGFSISSKSTDLYSPKVESDILVSKAKSDGETHFSTAKVSVIEFREHE
                     ESSMSKVTNSENSEETYVNKSSNGKDVLYTDSGEVSIEQSGYNDNRFVISRKGNLLAV
                     NKEQYRTWIE"
     gene            complement(29921..30280)
                     /locus_tag="KR250_002460"
     CDS             complement(29921..30280)
                     /locus_tag="KR250_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBH0068389.1"
                     /translation="MEIKLFDSELKVMEVLWERGESKAGELVKILKEETGWNRNTTYT
                     VIKKLVSKGAIERIEPNFVCKALISKEEVQKQETETLINKMFGGSSEKFLSAFINKEN
                     LSSDEITKLKQIVEKLK"
     gene            complement(30485..31264)
                     /locus_tag="KR250_002461"
     CDS             complement(30485..31264)
                     /locus_tag="KR250_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HBH0068390.1"
                     /translation="MVSRQELKRVSKAQLSGNWGICAISMAVYIALIVLISIVGLPFE
                     PLTTIVVAVIAFCVSAGFTCMFLKITKDEKVKIGDIFVSGRIYLRSFGFYLFESIIVY
                     IYSFIITIIGAFLSFGVLLTSGAIEYLSSDNVGIGTVISTGITLVLIILVLMLPVYIL
                     LLYVSQATFLICEDKDNMGVFKSMGASLDMMKGNVLKLFVLNFSFIGWYILSIATLGI
                     GLLWLIPYVGVTNCNFYRQLVKENPDVEDILLDKEKSYTMY"
BASE COUNT        10198 a         5764 c         2940 g        12583 t
ORIGIN      
        1 attaataagt aacacaatca aataaataaa tacaattaat atctaaatgc tattaataag
       61 taattcactc aaataggtaa atataattag tacttaaata aatgctactt ctcatatatt
      121 taaataaaca tagtaattaa cccttgagct atacctatta aaaaccctaa gacaagcccc
      181 aaaaactcta tatgttttaa ttctttctta gctattctta ttattatctc ttccaattca
      241 tataaatcta gttcattaat cttatcttcg accatttttt gtatatctat acgttcattt
      301 gctttattta ttatattttt actcaattca tctatacttt gcttaacttc agactcaatt
      361 atctctccta gataatcttg tatcatgttt tttataccac tcgggataaa tgaaactttc
      421 tcatgtatta taatttttac ccttgctttt atgtatctta caacttcttc cttatcttcg
      481 tcagtaatga tatttgcaag tatttcgtcc atagaaagaa attcttcttg aattatttct
      541 cctacatttg ctgctatttc tgctcttctc tttggtataa gacctactat ttctatattt
      601 aaaataggta ttttaatagg ctctattggt ctaaatatca gtcttatagc aactacattc
      661 gtaacataac ctataaatcc accaataaca gctaatatca gtatcctaat taaattatcc
      721 attataaaat tactccttat caattgtata tttgccagct atattgtatt aatcaagtag
      781 atattcttta tacttaataa cactagttat actatagctt tatatacaat tataagtctt
      841 ttgtgtcgat ttaaaaagaa tttttttaat tttatatgga tagcttttat atgcttagat
      901 tctagttttc cataattata ctaactttat tttcaaaaat tgcttctaca gtatctataa
      961 tttcctttga gcaattatta tttaacaaat ccaatatttc tatttttttg ggacatagag
     1021 ttaataaggt acttattaaa aagtcctcat aatttaaatt ttctcttatt accatattta
     1081 taatttcttc atcatctata ctgtctatct tatttccatt tttatcgtat aaaacaaatg
     1141 tattatcttc cattatatga actttaagca aatcaatctt ttcttcttga gtatctataa
     1201 agtattttaa aactcttata aattcatctt ggtctttctt tattaaatac tcctcaactg
     1261 caatatcacc tatagcagaa atatatttca taaattctct cattctaaat ctaataaaac
     1321 cttctacatt tataaaatca ttttcttcta tataatttct caccctagta aatactgttt
     1381 ctctcataaa aatctctttt ttagcaaata tgttcagtga atatatgtat atattatttt
     1441 tatcttcagg atatgtacgc ccatagcatt ttagaatgta ttctttcaaa agtttctctt
     1501 tcattatact tattatcata ctagttattt ccgttgatat ctcatcaata tttaaagctc
     1561 tttgttcagc tttagtacca ttaagtattt ttttatttaa cacttcttgc tttgcagaat
     1621 ctatcactac ttctacaatg tcattcgtat atattaatct atcgtttaca ttattattcc
     1681 tcaaaaattt catggaagaa atattatcct tctcaaccaa agttatcgct cctttcttgt
     1741 catgagagga actatagaac ctatatctag taaattttca cagctaagtg taactatctc
     1801 cttgttttta ctttctacaa aactttttat acgttcatag tcaggatgtt ttttaacatc
     1861 tccacaaaaa gctagtttat ccttagaaat caaccctgta catccaccta taaatccata
     1921 attcatctca aataaattta tgtgaccttt ttctatcaat aaacactcta tttctgtatt
     1981 cttcatactt ttccaaatac ctttatctga tgttattata gacgttctat ctactataca
     2041 gattgagcat ttagaatagc cctgctctat atttatcttt ttaagtttat tattttcaat
     2101 ataatctaat attgctttgt cagtatactt aaaattgtgt atagcatagt caccaactat
     2161 cgcaatattg taaggtatat cataagggta tctatttaat aaattagctt ctcccttaat
     2221 tatattaaat ttatatctac ttaataactc tttgtattga ttatatacat tcggcgcaac
     2281 aattatatct ccttttccta aattacaaat acatatatcc gggtgatatt ttatgctttc
     2341 atataattct ttacattcta ttgtttttat tatctctata tttcttaatt ttaattcatt
     2401 ttccattaga taactaattc ttttgtccac caaagctaga cacaaatcgt catctagtat
     2461 aaatggttct cttgtaaatt ccatttttta ctcctttttc ttttttttga taaatttttt
     2521 atgtgaatat aaataaagtg tgtagcagaa tttttctgcc cacacactta ttgttataat
     2581 tttatatagt taatctaagc ttaattagta tagctttgta cagttggttc aaatttaata
     2641 aatatatctt tttatcacta atctaaactt aagcagtata ttccgtacag ttagcacaaa
     2701 tttaataaat atatcttttt attgctaatc taaactcaat caatatattt tgtacagtta
     2761 gactaaattt aataaatata tccttttatc accaatctaa acttaagcag tatattccat
     2821 actgttaaca caaatttaat aaatatatct ttttatcact aatctaaact taatcaatat
     2881 attttgtaca gttagcacaa atttaataaa tatatccttt tatcaccaat ctaaacttaa
     2941 gcagtatatt tttataatct tctacatcaa taattaatat gtatctttca ctttgttata
     3001 ctcttttttt atgtattcca aaacatctgg tggtatattt ctactgctct ttgaagtcaa
     3061 gccaagttct ccatctttta ttttaaattc cattacttta cttcccttaa aaccatcttt
     3121 tgcaacgttc tttatcagtt catatacagg ttgttctaca tcttttataa ttgaacttat
     3181 tacattatct ggagccatat cgtattggtc tttattaata gctataactt tacctccatt
     3241 ttctcttgct acctgaatag ctcttttact atcatcctct gtagctgaaa atacaacatc
     3301 tctatcagaa tctctcattt cctttgcaat agttctagct gattcagggt tttcaaatat
     3361 atttgaatac ttaactagta actcagcatc tttatttgaa gttttaagcc cctttctaaa
     3421 tccagattca tacttatctc tactagaacc ctcaactcct cctataaaac caaccttccc
     3481 agtatcagtc attttacctg caataagtcc tgctaaatat ccagtaacat tatcttgaaa
     3541 tgatacagaa attacattag ctggctgaac tttataagta tagtcaacta ttgcaaattg
     3601 ctgtttggga tattcagtag cagcttcttt aattggctct gcaaatctgg ctccaactgc
     3661 tattatcaaa tctggttcat acttatgcag tttttctata ctctctttaa aacttttaga
     3721 agtctttggt ttttcaagtt ttatcctaac atcaaaatct tcgtcagccc tttttaaggc
     3781 tgatagtaca ttgttattat atgattcgtt atttaagttt tcggtatcta acaatactcc
     3841 aatagttttt cttacatatg aatcatcttc tgtgtcatct tctttattct tacttttact
     3901 atttgattta tccttttctg ctgaagttgg caaactttta gactcactct gtttcgattt
     3961 agcactatct atattagaat tattcttatt tactttatca aacccaaaat caaaattaca
     4021 tatagctaat ataaaaattg taaaaactac tacaaagcaa gccatcaata tcctttttat
     4081 tttcacaaaa ttcactccct gatttagata ggtctgacct acatctgcat atgtctttct
     4141 atatatgtct attataacat ccaattcacc tcataactaa agtcataagt attcttggat
     4201 attatagtag attatttatt gttcttatta acatattcta acacatctgg aggaatattt
     4261 tgagaactat tttgggcaag cccagtactt ccagacttaa ctgtgttttc tataactttt
     4321 ccgcctttat aatttccttt tacaaaactt tgtatcagat tataagtagg attctcaaaa
     4381 tcttttacaa tagaacttat aacattttct ggagctaact catgttggtc tttattcgtt
     4441 gctataactt ttttattttt tgcccttact gcatctatta cagcttttga atcatcttca
     4501 gtagtagaaa aaataatatc cactccatta tccatcattt ttttagctat agattctact
     4561 gatttgctat ctttaaaaac atctgcatac tcaactgaaa tatcctttat actactatta
     4621 gaaaatttaa caccctctct aaaacctgat tcaaatttat ctctactaga actctttgtt
     4681 cctcctataa aaccaacctt atcgctttca gtcatttttc cagcaattaa tccagctaga
     4741 tatccagatt tattatcttc ataagaaatt gaagttatat tagaaggttg cttgtcatac
     4801 tcatagtcta taattgcaaa ttgttgcttt ggatatttct tggctaccat ctccaatggt
     4861 tttgcaaatc tagctcctac agctataatt aaatcgtaat tgtcattaca taaaatatct
     4921 atactattag aaaaagttga ggaatcatct gattccactt tgtttgtatc tatattaagt
     4981 ttcttttgag cattgtttaa tgctaataaa gctgaattat tcatagcttc attatttgtt
     5041 ccttcaatat ctagtatcat tgatacagat ttttttgaat tacttttatc gcttatatca
     5101 cttttattat ctttgctaga gctaggatta ttgcttgggt tattagcaca cccaaccaac
     5161 attccagcta tcattgctac agttaacatc aatatcaata ttcttttgaa acgcattaaa
     5221 tttcactcct catacattta cgatattttt atatcctatt tacttttaca tcctatttac
     5281 ttttacatcc tatttataat ttttttatat acaatttaca agtacatttt gaaatttttt
     5341 taataaatct tactataaaa aagtgtgcaa tagattctta tctatttaca cacttcccta
     5401 caaactaaat ctaatatatc aacacttaat ttcatccaaa ataaatccaa tttaacatct
     5461 caataattaa tattctatcc taatttaata aaaaaataaa taatgtcatc caaaaatata
     5521 ttatttattt tttaagcata tgataccagt aacaccagga ccagcatgtg cacctataca
     5581 cacaccaatt tggaaaaatc taatttcttt aggtcttaat tcatcttgca taaccttaga
     5641 taattgttct ttttccttaa aatcatcact atatcctata tatacaattt ggtctgataa
     5701 atcatcacca caatttgatt ttatcaattc catcatctta gaaattacat tcttctttcc
     5761 tcttacttga gatatttgcg aaactaatcc atctcttatt tcaagaatcg gttttatatt
     5821 taatatactt cctattgctg ccttagtaga tgatattcta cctccctttt gtaaatattc
     5881 caatgtatct actgaaaata taacaaaagc cttctctttt aattcatcca aaacaggcaa
     5941 tatttcagca acagactttc cttcacttgc aagttctcct gcttttatta caagtaaacc
     6001 agttcccata caaagcaagt tagagtcata tgtatatatt ttaccatcta catcattctt
     6061 tgccattaca gcactttgat acgtacctgt agctgtggca gaccctgcta tataaagtat
     6121 ctctctatcc tcatttgtat atttctcaaa aatttcctta aattgggcat aagtagcttg
     6181 agaagttttt ggatatacat tttcttctct aagcttttta taaaattcat ctccagatat
     6241 atcaattcta tccttgtatt ctctttcctc cagtataact gttaatgaaa ctatatctat
     6301 atcatattta tctaacaaat cttttggcac gtccgataaa ctgtcacaga ttatctttag
     6361 atttttcatt aagtatgctc ctttctcatt taggcttgtt gaaactacct catatttggg
     6421 tatcctattt gtcttaaagc ctcgtacact acaatagcaa ctgaatttga taaatttaat
     6481 gacctagctt tttcaacatc caacataggc actctaatac aagtatccat atttgctttc
     6541 aacaaaggtt ctggtaatcc ctttgtttct ttcccaaata ctataaaaca attttcttca
     6601 tatttcacat ctgaatgtga ctgtttagcc tttgttgtag aaaaaaagta tttactattt
     6661 ggatactttt cttgtaattc ttcaaagcta tcgtaatatt gtatatcagc tatatcccaa
     6721 tagtcaagcc cacatctttt taggtgttta tcatccaatg aaaaacccag tggttttact
     6781 aaatgtaaag ttgaacctgt tgctgcacag gttcttatta tatttcctgt attttgaggt
     6841 atttctggtt ctactaatac tatatttaat gacattttaa tcctccctaa gtatcgcttt
     6901 tatttatcaa attttatttt ataaacagaa taacaataat attatactat tatataaaat
     6961 aatgttaata ataattttta tttcatgtat ataatagttt tttatctaac attttcacaa
     7021 gtattattat atcagaaaat ccctcttgta caacttactt tctaaaagtt atttcgtttc
     7081 attaagttct ttataataat tacaatcttc ttttattggg caacttgcac attctggatt
     7141 tctagcttta cacattcttc ttccatgaaa aattaataca tgatgtgagt gtgaccatct
     7201 ttcttttggt atagcttcca taagtgcaaa ttctgttttt tgaggatttg gctcgtccac
     7261 tattcctatt ctattgctaa ctctaaatac atgagtatct acagctattg ctggatgatt
     7321 aaaagcgtta ctaagtacaa cccctgccgt ttttcttcca acaccaggta attttataag
     7381 cttttctaag ctatcaggta cttcaccatc atataattca catagctgtt cagatgtatc
     7441 ctttattttt tgagacttgc ttttgtaaat accacaactc tttatctctt tactaatttc
     7501 ttcaatgctc aaattagcaa agtctctagc tgtgttgtac tttttaaaca actctgaagt
     7561 aactttattc actcttacat cagtacattg tgctgataaa atagttgcta ttaaaagttc
     7621 aaaggctgtc ccataattta actcacattt agcatctggg tacagttttt ctaactcgtc
     7681 taaaatctta tttacgtctt tttcatttgc tgactcattt ttaattttaa acttactttt
     7741 agtttcttta cttgtttttt gtgtagcctt attagaacta ctcttctttg tttttttctc
     7801 cgtttcacct tttttagtac tttttttact tactttgata ctttttttat cttcttcaat
     7861 atccataaaa ttctctatat ctttctcatc aattatctca tcttttttac caatcatttt
     7921 atctatttct ttttcattta tctcttcctt ctcaattata tctttcttat tcattccttt
     7981 tttatttatc tcttccttct caattatatc tttcttatcc atttcttttt tatttatctc
     8041 tttcttctca attatatctt tcttatctat tcctttttta tttatctctt tcttctcaat
     8101 tatatctttc ttatctattc cttttttatt tatctcttcc ttctcaatta tatctttctt
     8161 atctattcct tttttattta tctcttcctt ctcaattata tttttcttat ccattccttt
     8221 tttatttatc tcttcttcca aaattacatc tttttcattt atctctttta gatttacatt
     8281 tttcataatt atcccccttc aaaataccaa tttataaaac gctatttata aatagtttaa
     8341 tcatatcaac aaaaaaataa acatcaatat ataaaacata caaaataaga atttaattta
     8401 tatatattta caaaaaaagc aaaaacctat taaagttctt gctttttatt atacataaat
     8461 acccattaaa ttctataata aacttattct tttgacacag tttgtatctt gctgaaagat
     8521 attaattttt cttttaccaa tttctttatt tcttcaaatt taacatctgt caatatctct
     8581 atagcctctt ctactctttc aacagtatat atcttaaatt ttccatctcg cactgcatca
     8641 ttaacttcat ctgataaaac gagatttctt tgattttttc taggaattat gacgccttgc
     8701 tcaccattta accctttagt tttacatata gaataaaatc cttctatctt ttcacttata
     8761 ccaccaacta cttgtatatc acccttttga tttatagaac ctgtaacagc aatattttgt
     8821 ttaattgata ctccactaag agatgataat agtacatata attctgcaga agatgcacta
     8881 tctccatcta ttccaccata attttgttca aaacaaacat atgcatttag tgacaatagt
     8941 aaatcctgtg caaaattctc agaaagatag ccacctaata taagtactcc tttgttatgt
     9001 atagagccac tcatatttac ttctctttct atatttacta tgcctttact tcctgggcta
     9061 gtggtcgctg taatccttga agctctacca aaagagtatt ctcctgtact taagactgac
     9121 aaaccattta tcactcctac ttttcttccc tctgtttcta ttagagtaaa tccattttca
     9181 attgattcat ccatatgtgt ttccactcta ttgattcttt ttctcttttc acttattgct
     9241 tttttaacat cacactgctt tgtatactcc gaatttctaa actctgcata agcacttcct
     9301 tctattacaa cttccattat tttattaaac ttagttgata atttttctaa atcaccagat
     9361 aatctagtgc taaatttaat aacctcttca actgctgatt tagtaaaatg ccttaaatta
     9421 ttcttatcac attggtatgc tataaatctt gctattcctt cttcattatc ttcattttta
     9481 tccatttcac tatcaaagtc tacaaatact ttaaaatatt tttcaaaatc ttcatcatat
     9541 ctatataaaa tatcatatat atagtggctt ccaatcagta ctaatttaac atccaatggc
     9601 atattttctg gtttaattgc tgtttgtgtt tccaatatta cttttttagt ctgcaaagtc
     9661 tttttaagaa tttcccaaga cattgtatat tttagtaatt ggtctgcata aagtactaaa
     9721 taaccaccat tagccctatg aaatgctcct gataatagtt ttgtgaaatc agtctttata
     9781 tttccatttg cataatcata ctctgccttt ccaaatagat ttgctggact tgggtttatc
     9841 tctactacaa caggtgctga ttctttattc tttgagcttc cattgtctac aaataggtta
     9901 attttatatt taataaaatg ctctttatca tacttatctt ttaactcctc ttcatctaaa
     9961 tagaataaat acatatattc tattatatct ttctttatat tttccagata agtttgaatc
    10021 ttatcataat tttcatattt ttcacacaat ctcttgatat gaggttctac tacaagttta
    10081 gctatctctt cttcaagctc taacactgct ttttcagcta tttcttctaa gtttctaatt
    10141 ttatatacga cctgtatcgc catattttct agttctcttt tagctttgta aaactcttca
    10201 tcagactcat cactttcatc ttcctcttct ttcaaaggga taaaaactat tcccatttta
    10261 ctctgtttta atttaaatcc tttttcttct ccatattttt taatttgctt caaaagcata
    10321 tctttttcta tttcatattc atccagaagt ttattttttt ctatttcaaa attttcactt
    10381 tcaaacgcat ttttaaaatc ttctaaaaga ctttcaatta atttctccat atctttctta
    10441 agttctcttc caaacccctt ttctaattca actataatag gctctctagg gttttcaaaa
    10501 ttatatatat aacaccaatc tttatgcgta ttttttttgg atgcatactc atttaaaacc
    10561 tttaaggcat aagtactttt accagttcca ggttcaccag ctatatatat gttgtacgct
    10621 ggattattta ttttcaaccc taattccata gcttctacag ctctttcttg acccaatata
    10681 taatctagtg gatttatttc agatgtatta ttatatttca atattccccc acctccccat
    10741 atttaacttt aatgatataa tatgactttt acacaacaag tgttaaagta aatacacatt
    10801 aaagtatatg aaacagactc ttattattag tcatcatact tatctaaaat tatttccata
    10861 tttctcttta ttatatttct acctctccag ctaaaagctc tatctccata tctcctttta
    10921 aattctttat ttgaaatatt ttgtatctca tcataatcca aataatctat tatattttct
    10981 ttaaattcca aaatatgagt tgttggtatt tcaacattat gtggacatac ttcttgacaa
    11041 atatcgcatc caaatacctt tttgccattc tttaaaacaa ctttttcttt ctctgacaaa
    11101 tctccttttt tttgagttat atatgataaa cattttttgg ggttcatatc ataatttccc
    11161 aatatagcat ttcccggaca atattttaca cacttaccac atttaaaaca acttttttct
    11221 agtggtttat cttcttcaaa tttataatta tttactatat accctataaa tacgtaagaa
    11281 ccatattcat cagttatgat attattattt attccaaaat atcctattcc tgataaataa
    11341 gctaaatacc tgtcaactaa tggtccatta tcagcaaata acttgtattt aaaatctgtt
    11401 actgttttag aaatataatc acaaatttgt tgtaaaaagt cctttactac aatgtgataa
    11461 tcctttccat gacaatattt tgataaattt gaatttttat tcttttcttg tctatttata
    11521 tgatatggaa atgcacagac tattatagag ttagcatcct tcattatatg tcttgggtta
    11581 attctgctct caattattgg ttcttccata ccagttaaat aacctttttg ttgtctgttc
    11641 ttaagtatct tttctaaatt atcatattta tctacccctg ctactcctac acagtttaat
    11701 cctattaaat tacaaaatgc ttttaattcc ttattatcca taaaattatt cctttttaat
    11761 ctaattgact ttttaaattt atttaatttt tttagtcttt ttaacctttt ctagctacta
    11821 actcttttaa atacttaatc tttttagcta ttcaattttc ttacctgctt aactctttta
    11881 gatacttcgt ctttttatct atttaatttt tttacctact aactctttta ggtacttcgt
    11941 ctttttacct atttaatttt ttacctacta actcttttag atacttcatc tttttaccta
    12001 tttagttttt tacctactaa ctcttttaga tacttcatct tttttattta cttatttaac
    12061 ttttttacct actaactctt ttagatactt agcctttttc atttacttat ttaacttttt
    12121 tacctactta actcttttag atacttagcc tttttaatct attcaacctt ttttacccac
    12181 ttaaccttct taccttttaa tataaactct aaaacttcaa atatcattag ttatattttg
    12241 acattctttc tatagttaag caaaaagatt gattttttct tataattaat atcccattta
    12301 cattgccatt tattaagtct ttacttgact tatatggatt tctttttact ctctttacta
    12361 aacttatact tattaatatt atgaaggtct tataatagtc taatatgaat atgcccatca
    12421 tatatcaaat tttaaaattt gtaagcaaaa tacttatctt atattactac atctaaagtg
    12481 gaaaatttta caaacaagct ctgtaaaaac actttaaata acaatttgct tttaaatcat
    12541 atacagatta taataaatac tgtataagga tggtgaaaaa tttgattaaa ataacaattc
    12601 caggtagacc tattagtaaa tctaatttta agcttcataa cataaatggt caagcatgga
    12661 tgccttcaaa gggaaaatat tctaagtatc ttgcatacga aaacatgatt gcaggtttta
    12721 taaaccaaca gtatcaaggg gaaactgttg aagaaaatct aataacagtc ttgaaattat
    12781 tcttcccaaa taagagaatg ggggatttac ataattatcc aaaaagtatt tgtgatggta
    12841 ttgaaaaaag tggaataata aaaaatgata aacaacttaa gccagtactt ttatttgatt
    12901 ttatagataa agataaccct agggttgaaa ttgaacttta tccagtatct aaatacgata
    12961 tatcctataa tatttttgaa aaataaagat tttatgtgtt attcgacatt atattgatac
    13021 tccagtattt gaaactatac taatacaaca ataataattg tgttatgtct atactcaaac
    13081 aaatgttttt aaaaagcatt aagtaaatat ttaataagta ttaaacaaat attaaattta
    13141 tataatagct ataactatgt aaatttaata tttgtatata agaccacatc taataaaaag
    13201 aaatcggagt aaatttaata caccgatttc aataaataaa atctatccta gcttttcaac
    13261 aacagtctta agcatttctc ttataggaag ttcatatgga catctactct cgcaaagccc
    13321 acagtcaata cactcagtag gtttaacatc cataacctca tatttttctt tagcccactc
    13381 ttttaatcca tatctagtat aataaccttc acataaaaaa ctcaatggta tatttattcc
    13441 tacagcacaa ggcatacaat attcgcatct tctacagaat tttttaccta aagaatttct
    13501 tatttcttca atttccttat tatctttttc atctagcaca agattttcaa gtacagcgac
    13561 attttgtcta acctgttcaa cactctccat acctggtata actacatcta tgtaatcttt
    13621 agatagtata tatttaatag ccagtgttgc attgtctaaa gcacctccag ctaaaggttt
    13681 cattactatt atcccaattc ctttttcatg agctttttta aacacttcat ctgcttgtcc
    13741 ttcaactata ttgtaaggga attgtatagt gtcaaatttt ccatcttcta tagctttttc
    13801 tatagtattt aaattgtgac tagttatacc aatatgtttt atcttacctt gctcctttgc
    13861 ctctaaaagg gcactataag ccattttatc tttaaaaaga ttatcatact cctcttcttt
    13921 aacattatgg aattgatata aatctataaa atcagtttta aaattattta aacttatttc
    13981 aacatctctc ttcattgaat cataatctct ggacatactt ttagtagcta agaaaaattt
    14041 atctcttttt ccttcaatgg caattccgat tgcttcctca cttattgtat accctcttgc
    14101 actatcaata aagtttattc cttgtttttc taactcattt ataactaagt tagtatcatc
    14161 ttgagtaatc ctttgtattg gaatgccccc aaagccaact ctctttattt tcatgttagt
    14221 attgcctaaa tttctcatat ctttccccac ctaactttta ataatttttt atataatata
    14281 aatatgtcat ctttaattat acaacaaatt atgagaatct ttgcaatata tttacatact
    14341 attcatagta taattatatt ttatatcagt ctattcataa tataatcata ctttataccc
    14401 agttaatttg tatacaattt ttgttattcc tatccattaa tttaaattat cacatttcat
    14461 gattgtatct ccaccctaat tatgttgagg gtgaagtatt tcacatattt ttaataaagg
    14521 tttatttact agatagtatt tcttttatca ttttaagctc tttcttgtga ccttcatcat
    14581 caaaaggagt ttctaagaaa aatggtaaat gttttagact aggatgttcc ataaaatcaa
    14641 ttaatgcttt taatcctatc tttccttccc ctataggagc atgtctgtct tttttatctc
    14701 caaatggcat catactatca tttagatgga cagttttcaa tctttctatg cctataacct
    14761 tatcaaattc ttccaaaacc ccatctaaat cattcactat atcataacca gctgaaaaaa
    14821 tatgacatgt atcaagacac acacctattt tttctttatg ctcaattttg tctattatac
    14881 tttttaattg ctcaaattta aaacctattt cagttccctt tcccgacata gtttcaagaa
    14941 gtatagttat attttcatca ccctttatgg cattgttcaa tccttttact attctatcta
    15001 tgccaaaatc tacaccacca cctacatgac ttccaggatg aaaacacatg tattcaattc
    15061 ctaatgagtc catccttctt atatcctcat ctatcaccct tgttgcaaat tcgtatacat
    15121 catcctttgt accacccaga ttcatagtat atggtgcatg tgcaagcagt ggtgcaaaat
    15181 tattttcttt tctaatttct tgaaatttaa ttatatcttt ttcatcaaat tctttagcat
    15241 ttcctcctct tggatttcta ctaaaaaatt gaaatgtatt tgcaccaata tatacagctg
    15301 tctcagccgc attagcaaat ccttttgcta ttgaaagatg tgttcctaat ttaatcatat
    15361 taatctcctt ttatttgtac tactacttgt actcaatttt aattttgaac tactccacct
    15421 taactacatt tagccaacat atgaagatgg agattcctat ttgatagtac tttttcacta
    15481 aaaattcgta agctctaagg gatgttctat cccaactaat ctcagtcctc ttttaacata
    15541 tttctagcat tgatttaatt tatattaatt tttaatttta atatttacac tttaattata
    15601 cccattttaa tcaatttcaa atattataat cttacatact ttgtatatta tctaagcact
    15661 tcttagatag tgtatacatc ttacactata catattattt acaaatacaa taggtataaa
    15721 aaaacaaaaa aatagagatt aaaaatcatt gttttaccca taccaaaaac tattttttga
    15781 ttattaagta aattttgatg ttcaatctct aatattatat ctttaattaa tccataatta
    15841 attctctaac taggacattc ctcctcaaga gtaggaacta taaccatagc tatatcatac
    15901 ttagaacctt caacattgtg ttgtctttta tttaaaattt catctatctt tgacttcata
    15961 gtgtcactaa gttcaactaa ttcatcatga gtaagagaaa tatcatgata atcaactata
    16021 ttttcatttt ctaaaacatc ttcctctgct gccttataaa aaacttcact catattttca
    16081 aattttgata tgtacaattc ttctttttct tccccattgt ctaaagaaaa attaagtatc
    16141 ctattcccta taaccacatt ttttgcactt ggataatagt acttttccac aatccccgac
    16201 tttatttttt cctcaactaa ttctaaaata ccaactttgt ataacatttt tatatggtag
    16261 tttatttttg catgaggttc ttccaataat tgagataatt gttttgctga caatggcgat
    16321 ttgtcaaatg cttttagaat atctattcta cgtggatgcg caatagcctt tatgcactcc
    16381 aaatctcgta ataccaatac ctctttcatt gacccctcct aaaaaattta tttaattcat
    16441 atttctttac aatttaatta tatattaaat tatatgttgt tgtcaaaaaa atcctttctt
    16501 aatattttaa aaaatattta atttgagttt atttgacaac tcctctaaaa ctttaactcc
    16561 tgcgacgctg ttaccttttt tgtcaagcgc aggtccataa acaccaatcc ccatgcgtcc
    16621 cggcacagaa gccattatac cacccccaac tccagatttt gcaggaatgc caactttaat
    16681 agcaaattcc ccagaagcat catacattcc acaagtcatc ataaatgtct tgacaatcct
    16741 agaaaccatt tcactcatta aatgctcacc attttctata tctactccat aatttgcaag
    16801 atttattcca attctcgcta aatctacaga atctatctca attgagcatt gtttaaaata
    16861 aaggtctaga acatcttcta cattccctct gataaatcca tcacttttca ataagtaagc
    16921 catagctctg tttctatctc cagtcatttt ttcagactta tagacatcat agtttattcc
    16981 tatattatca ttttttgcta actttctaaa gaaactaagc attctttctt ctttttcttc
    17041 taaacaactc ccatttataa gggaagtggt tactatagcc cctgcattta tcattggatt
    17101 acagggtttt tttgtgtcat taatttcaag tttcattatt gaattaaatg ggtccccact
    17161 tggctccatt ccaacgtttg aaaaaacgtc ttcccaatca ttatccataa gtgccatagc
    17221 aagcacaatt ggttttgaaa tactttgtat agtaaacttt gtgctacagt ttccagcact
    17281 ataaagatta ttttcgctat ctatgataca aattcctaaa tcatttcttc tagcgttttt
    17341 taattctggg atgtaactag cgacttgtcc ataattagta tacttcttat tagaacttat
    17401 aatttctttt aataatgtct catctaacgt attcatatgc acctcttata ttatgttata
    17461 tattctattt tagcacaagt ttatactata ataaaacaat gttaaaaaaa attatacttt
    17521 aaaattattt tctacaagaa tcaattcatt tttcaaacaa tttaaatagt ttaaagagct
    17581 taataattta gtaattcaat aatttaacaa cctagtaatt tagcaatcca gcaatctaat
    17641 aatccagtaa tttaaatttt tataacacca aaaatttcta acccaacacc ttttactctt
    17701 ttactctttt actcttttac tcttttactc ttttactctt ttactctttt actcttttac
    17761 tcttttactc ttttaaaaaa taatcacata aactaacaaa atattaaaca aaaaagtaaa
    17821 atttaaaata aaaagcttgc ttcatacaaa agtataaaac aagccttttt atatatcaac
    17881 tcaaacttta aaatctgtat caatttaatt ctccacttta cacttaatat acttaacata
    17941 aaccaataac actacttgct atgccattta atcttcataa aacaatctat aatacacaca
    18001 ttaatcaact attttacttc ctccaataaa ttccctaaga attgaaatac ctggtatgtc
    18061 agaagtatcg tccaattcaa caaaatactg caaaaacttt agtaatctag tagcttcaat
    18121 atgtgcagga ttatcttcct ttatctcttc caacaaaggt ttcgcaaatc gttttaaaac
    18181 tgccaattca tatacacaag cagagttaaa tatagcatct gcctcctctt ggtacgggaa
    18241 tatattcttc ttttctcctc ttctaactga tgcccattgt aaaatagtat tatcagcact
    18301 atatcctcta aaattatagt ctctaacaat tcttcttatc attctcaaat cagcagtagg
    18361 gattcgatta tggtcatcca aatttatttg agtaagggca cttatgtata tcttaaattt
    18421 atcttcatct ggtatagatg atgttaaaat aggatttagc ccatgtatac cttctaatat
    18481 aattggctga tttgaatcta tctttatctt tttaccagtg tattctctct taccttcttt
    18541 aaaattgaat cttatatcat caatttcttc accagctaaa agtttcttta agtctaagtt
    18601 aaacttctct aaatctattg cataaattga ttcaaaatca taatttccat attcatctaa
    18661 aggagtatgt tttctatcta taaaataatc atctaaagaa attgaaatag gatttaaatt
    18721 atttactcta agatgtattg ataatctgtg agcaaaactt gtttttccag atgaagatgg
    18781 agctgctatt aaaactactc tcttattgcc atcctttatc atatccgcta tttgagatag
    18841 cttcttttca tgcaatgctt caaatgtcct tattatatct ccatattcat tgttttctat
    18901 tattttatta agagatacta ccttgtcaat tcccataagt cttgaccatt cttctgcttc
    18961 ataatataca cttgatagct taggttgagg tacaaatttc ataggaagag tcttatcttc
    19021 ttcactaggt cctaaaatta taacgcctcc attataaagc ttaatatcaa atgattttat
    19081 ataaccagtt gatggcaaca tataaccata aaaatggtct atatagtttc tacacttata
    19141 tactttagca ctatcatact ctttgtattt caaaagctct gccttttcat acatttcatt
    19201 attttcaaag atagaaatag cttcatttct tgtaacttca attttttcta taacataatc
    19261 gttgtttact atctctttca tcttgttttt tatattttca acatctgctt ctttaagctt
    19321 tctatctata tgaacttcac aatatagccc atctgatagt gaatgttcta ttgtaactct
    19381 gctatcccaa aagatttctc tacatgccat tacaaatata aatgacatga ctctaaagta
    19441 aactctctct ccatcttcat tagtagtatc taaaaaattt atctcacaat ccttcttaac
    19501 tctacaattt agctctttta gtttattgtc tacaactgca agtgttatat aacccttatg
    19561 ctcatcacaa aattcttttg ctatatctcc caatctgatt ccaggggaat tttcttctaa
    19621 tgaatattcc ttatcttttc cttctatttt taagtttatt tttttcactt caacatctcc
    19681 ttccttttga tgaatataaa ttttaattaa tgaggcatat caacaagata aagagaggta
    19741 tttaaccctc tcctacaata ttattcaatt tacttatagt atcttcaatg tcttcgtaat
    19801 ttatttctaa tactaaatat tctgcatcta atgttaagac ctctttaatc ccctcttcag
    19861 ataaaattcc tcttcctata ccaacatgtg tatcattgat accatcatta tcacttaagt
    19921 gtatcagtct tattttatcc tttaagttct ttacataagc atcctcatca attaaacagt
    19981 gtccagtatc gtaacaaaaa caaatcttag aatttttaat cctcttaaat atatattcaa
    20041 aatcataaga tatatttcca atatttgaaa aatccccacc ttttgaataa gtattctcta
    20101 tacatacata tgaatttgta tttaatttat ctaaaaaatc cacactattt cctaaatatt
    20161 tgtctctatt ctttctaact ctatttgtca taacatatcc caaatgaagg ttaaaatacc
    20221 ttaaatcaaa tccttttagt tcaaattcta tcttctcaat aaaatttatc catgaatttc
    20281 gtatatactc tatgttttcg caagtattta attccatagg caagtgtatc cctatagata
    20341 atcctaactt agaaattctc tctttatatt tacataactc actacattca gatatattgt
    20401 caattccaat ctctaaatga gttatttgct ttatactctc acaaatgttt aaagcctcct
    20461 caatattaaa caataaggac gctataccaa ctgccataca aatcacctac tcaaaaatac
    20521 ctatatctgt aaaaggaaat actcttaaaa taacttttcc tttaatattt tcttcatcta
    20581 ctaatcccac ttctttatat ctactgtcta aactaacttc tctattgtct cccatcgcaa
    20641 atacttttcc cttaggaatc accatatcaa tatcgccttc agtataatta tcatgtatat
    20701 atgaaacttc atttaataac ttaccattta tataaacctt agaatcctta atttttagat
    20761 ggtcaccagg aacacctata acccttttta caagctcttt tttccttcca ttctcttgta
    20821 ataaatcagt cttaaatatc attatatctc cacattttgg ttctgataac ttatatgtca
    20881 tcctgtttgc taccaaataa tcatgtgatt taagtgttgg gtacattgat tctccattta
    20941 ctaatgttgg ttttataaaa cgagttataa taaatgcaaa aaaaagtgca gtaataatta
    21001 tttttatcca ctctacaatc tcttctttaa tagtttcatt cattcacaaa ttctccttta
    21061 tagttatatt atctttattg ataatgtctc ttaaacacta tcttttagat aagtctacta
    21121 aataaggtgt tgtctttttt atatagacaa ctattgtgta tggtctggtg atttcagcta
    21181 atcagcgatt gctccaaatt ttatatgttt agtcaatagt tcctatctta gaaaatggat
    21241 acaatctgac tagaaccttt cctaaaatgg tatgctcgtc gaccaatcca acctcatcgt
    21301 atctactatc taaacttttt tctctattat ctcccattgc aaataatttt ccttctggaa
    21361 ctacgatatc aatatctcca tcagtacgat tattatgtat ataggaagtc tcatctaaca
    21421 acttatcatt tacatatacc ttggagtctt gtatttttac atggtcacca ggaacaccta
    21481 taactctttt tactaaatct tttttctttc cattttcctc tgttaagtcg gttttgaata
    21541 ctattatatc tttgtatttt ggctctccta ccttgtacgc aattctgtta actatcaaat
    21601 aatcacgttc aactaatgta ggatacattg attctccttt gactattgtt ggcactataa
    21661 aacgagttat tgcaaatgcc aaaacaagag ctatgacaat cacttttatc cattctacaa
    21721 cttctttttt aactgcttca cccactaaaa tcgtctcctc tgtgttaaat attaagtcaa
    21781 tctacagcaa aataatctac cataaaccta caatataaat attatacttc ttttttctaa
    21841 tagtttaaaa tacttttcta agacctttag gataatgatt cttaattaca ccagaagatt
    21901 cttttcccca acctaaaggt attccttcaa ccgaaaccaa aacccatcct ctactatcat
    21961 tagaatttac aacatcccct tttaaatatt taagaattga ttcactctgt acactgaagt
    22021 tttcaacatt ttttacatct tcaactttta agtaatgaga tagtgcatga gaaggctcaa
    22081 atctattctt ctttaaaacc cctaaatgca aaccatatct caatactttt agttttttcg
    22141 tatctggata tatttcagga agtaggtaca aattttctcc tcttaaataa aatttatttt
    22201 ttttattact ataaaaatca acctttaaaa atttcttttc aaactcccta taatccttta
    22261 tttctttatc taaatttttt atttttaatt ctttgacatt acattcttct tcttctaatt
    22321 tttttatttt ggcaacaaaa tgaccttctc cctttatctt atgaggccat aatctttcca
    22381 tttcaataag ttctgcatgt ggatattcac tgataaactc atttataaca tgctcatttt
    22441 cctcccttga aaaagtacaa gttgagtaaa ttaaaatgcc atccttcttt aacattttat
    22501 atccatctct tataatctct ttttggatgg attgacattc tactaccttt gcataactcc
    22561 aatctgatat ggcaacctca tccttcctaa acataccttg cccagaacaa ggtgcatcta
    22621 taataacctt atcaaaatac ccattaaacg tctttcttaa gctattagaa tcggtgtttg
    22681 ttataataca atttctcgct ccaaatcgct ctaaattttc ccctagagcc ttaattctac
    22741 tagcattaat ttcattagaa accaataaac ctgtatcgtt tagcttagat agtatatatg
    22801 tagattttcc accaggagca gcacacatat cgagaacttt ttcaccctct ttaatatctg
    22861 cctttggtat tacactcatt gcagaaggct cttgcaaata gtatgctcca gcctcatgca
    22921 gtggatttct accaggtctg tcaattttat catcataata aaaaccttct tctgcccatg
    22981 gtatttgatt caaatcaaac aactccaaat caaataattt tttcttgtca atttttaaag
    23041 tatttattct aagcccagta gtcttacttt cctcataact ttttataaaa tcatcatatt
    23101 catcttttag tatttcttca atatcttcta aaaattcatt tggtaattct gtcaaatcta
    23161 taatccccct tcaatctgct ttacttgtgt gtcatttatt atactttaat tatttatgtc
    23221 tttcctttaa attttatttc ttcttctttt tcctaagatt accgaaaaac acttttttag
    23281 agtgattcat atatttctca agtttatcaa gatttatctt ccacaaaatt attacataaa
    23341 ctataagtaa tataatagcc catctatatt ctatcatcaa tatgaaatca aaaactccat
    23401 gtaataaaat tggaattaac actgccataa acaaatactc acgtctttta tttttatttc
    23461 cctcaaattt atacttagaa atatagtagc ccatagtaat tgcaaacatt acatgagctg
    23521 gtatagagat aactgcccta ttaataccaa cttgcagtgc tagatttcta ctttcagaaa
    23581 aaatataaac catattttca attgttgcaa atcctaaagc taaaaataca gagtaaataa
    23641 taccatctaa cttttcagta aagtgttttt ctttaattag ggcaggaatc agtatcagag
    23701 cttttacacc ttcttctaca agtcctgcaa ctacaaatga tacatatatt agttctgaat
    23761 acccttcaaa tacattaaac ttcatcagta aattttctaa aagtataatt ataacactta
    23821 gaaaagcacc tattaaaaaa aatttaatta gatggattag gggttctcta caatatctat
    23881 ctttagacct tatccaaaac attcctatca atataggaac tattgctaat aaaaataaat
    23941 ctagcttcat agattatttt cctttcaaaa tttttgattt atttatgatt tatatcatct
    24001 acttattttt aacttattcg tgttttaaat acatactaaa atatacatta aattatttta
    24061 aattatttaa atacaaatat actcaaatac tcaaaaaaat ttaaatttat atctatatta
    24121 tttaacaaat tttaagctat attcatatct tgaaatataa gggaactaaa ttcatttaaa
    24181 gaaagggtga tttgatgcaa gatggtttct taacagtaag cataattgat gctactaaca
    24241 atagacctat tcaaaatgca gtagtaaaca tatattcaat gtctaatggt agccaaagct
    24301 catcaacttt ataccaaaac ctaagaagta atgaatctgg tcaagtgact ggtttagtat
    24361 taccagcacc agatgtagat tactctctac aaccatctga tgttagacct tatagccagt
    24421 atatagtgga agccatagca gatggatatg agacagttgt catagaagga acacagttgt
    24481 tagctacaat tgaagcaaga caaggagttc ccatgtctcc tagaacaaga agtaagcgtt
    24541 ctttctcaag acaaagtgag ttgatttttg acataggaga acatactcta tatggtactt
    24601 atcctcctaa gattccagaa agtaacttaa aaccattacc acctcctact ggtttcgttg
    24661 tattagataa ccctgtagtg cctgaattta tagtagttca tgatggtttg ccagaagatt
    24721 catcagctcc aaactattgg attccattta aagagtacat taaaaacata gcatcatctg
    24781 aaatatattc aacttggcct gaacagacaa tttatgcaaa tgtaatagct ataatatcat
    24841 ttactttaaa tagagtcttt actgaatggt atagaaataa aggatataac tttacaataa
    24901 cttcaacaac agcatacgac cataagttta taaataatag aaacttattt gaaccaatta
    24961 acgtagttgt agatgctatt tttaatactt ttataaaaag acctcctaca tcaagacagc
    25021 cactattagc acaatattgt gatggacaaa aatcccaatg tcctgaccaa atgactcaat
    25081 ggggcagtaa agacttaggt gaccaaggat atgactatga gagtatacta agatatttct
    25141 atggagatga gatagtattt gaaagagcgc ctatagtaag tggtgtacca gtgtcattcc
    25201 ctggtactac actacaagtt ggttcttctg gacagtatgt tagaaccata caaaatcagt
    25261 taaatgctat atctaattct tatccagctg taccaaaagt tatagaagat ggtatctatg
    25321 gaacagatac agaaaacgct gttaagatat tccaaggaat ctttggttta cctcaaagcg
    25381 gagtagtaga ttttaaaaca tggtatgaaa tatccagagt ttatgtagct acaactagaa
    25441 tagcttcttt aaatccttta atttaaagct tgatttagaa cataaactca aattattgaa
    25501 taaaattatt aatttaaaat aaaaatggct gttatgaact aatatatacc ataagtatta
    25561 cttataagta ttgtagtctt aacagccatt ttttagttat aacttgtttt tttagaatct
    25621 taaaacttta aatgggggat tttaagcttc ttaaaaacag tgcttagcaa cttagatttc
    25681 tttaaaatat tatatttatt tttaagttta ataaaatgct attctaacga aaaacctact
    25741 ttttatttaa aatctcactt attatttaaa atgctacttc tcacttagag tattactcct
    25801 tattatttaa aacactatct atcacttaga gtattacttc ttattattta aaacactatc
    25861 catcacttag aatattactt cttatataaa ttattattta ttataaatac aaataatatt
    25921 aatttaaaaa gttattccta atttaaataa tactactatc ttcatcagtg aacctaaact
    25981 gcaactttag acttattagc taaagccaat attagaccta aagctataga tgcaaatgca
    26041 gaagcaatta ctctatactc agcagggaat aagaatacaa ttgtatgaca tggtatccag
    26101 aaaaatggta tagtttttaa aacactaaaa ccaaggtaag cattccagtc cacaatattt
    26161 accacatctt taagttttac cttaatcccc tttccaacct catatttttt atctataaaa
    26221 cagtccataa ccttatggaa taaaaataca gttggtgaca ttagtatatt catagttgca
    26281 ctcttaaata gagcatttgc aaaagctatt ccctcaaatg gaagcatacc atccacttgt
    26341 gcttgaccta cacctgatga gtagattgac attccatatg tggtaactac acctattatt
    26401 ccccaaacta ttgctctata aaatactcct actggtattt tccaatcctt atacaaaatt
    26461 ctagcaccaa gtaaatctcc cattgtagct aatacagcaa actttaaaaa acctccaata
    26521 tatggatgtg cacctgttgc ttccataaat acttctctac tacttggtat tgctaagaca
    26581 gcaataatac aaataagaac tccactccat ataaaatcgc cttttttcat atttataaat
    26641 actatttaat atataaatag ttctcctttc tattaaagat ttatttaatt tacaatctat
    26701 ctacaattat ttattaatca cttattatta gatattcatt ttccatattt aaatttattc
    26761 tatcccttta tctatttatt ttcttactac tctatccacc attatctata tatttatttt
    26821 acgattactc tttcacccta accctttaat tatctatttt ttctgatatt cttatctacc
    26881 atcatccata tatttatttt acgattactc tttcacccta accctttaat tatctatttt
    26941 ttctgatatt cttatctacc atcatccata tatttatttt acgattactc tttcacccta
    27001 accctttaac tatctatttt ttcttactat tcttatccac atcatccata tatttatttt
    27061 acgattactc tttcactcta accctttaac tatctatttt ttctgatatt cttatccacc
    27121 atcacccata tatttatttt acaattactc ttttacccta accctttaac tatctatttt
    27181 tttctggtat tcttatctac tattatctgt atatttattt tatgattact cttttaacct
    27241 accccctata ctttatagta atatgttttt aacctttttc attattccca atatgcttta
    27301 taactacaaa tactctcccc ccttaaaaat aataataaaa ttaatcaata aatttataat
    27361 catattatta ctctatgtat taagcaatta ctatgccaat aaactttgat ttttttaaac
    27421 aaatcttaaa aatcctaaaa tgctaatttt ctttaacttt taatgaatct aatagataac
    27481 atttaatttt cgttatattt ttcagaatat ttctattatt atttttaaga aaaaaaaata
    27541 atgttaacat ttcttaacat acattaaaaa atgttaacat tataaattgg gtttaatact
    27601 tttactttga ctcgatttac ttttattcta cttcttactt tatttttaac ttagacttta
    27661 tttttttatt acttatttga gtcttattta ttttattatc actccatatg ttcaattaca
    27721 tttgtctaaa ttctttgata cttttattac ataattttaa caagcttaag tgttatttgg
    27781 gtagtgcagt tgataattat ttaaataatc atcaactgca tgtttatatc attacagtag
    27841 cttaatgtat ttaatcatac ttacttagga ttttgaaata tcttagagat tatatttaac
    27901 attagtcaat tgctgcataa ttgtaagcaa ttgaaatttg agacccatct tcattttcta
    27961 tagtgatagg cttcataatt tttaatcctt cagtttttat cttttctaat tcttgttcca
    28021 ttttcttaat tgtttcctca aggtcttttt gacttacatc tttgactgca ttagggtctt
    28081 tagcatagtc ttttaattct tgaatatgtt tagcatactc ttcataagta taatgctcaa
    28141 caccatactt ttccaattca gtagggtcag ttattcctga ttttattaga gattcaagtt
    28201 taggtaaagc aatattagat gaatcatttc catttgcaaa tactgctcca gttcctccta
    28261 gtaccattgc agataccatc attgcactta taagggattt ttttattttc ataaccaatc
    28321 ttcctttctt agagatttat ttgggtagtg tagtttgaaa tgactattgc aatagttatc
    28381 aaccacatcc ttagactatc acagtagtca tgtttatgtc aagccttttt aaaaaacttt
    28441 tcaataaaaa atactatctc aaaacaaaat taattatttt gagatagtat tttaattcta
    28501 aatcttttat attactaaga tttatggtta ctctatccat gttctatatt gttctttatt
    28561 aactgctaac aaattccctt ttcttgatat gacaaagcga ttatcattat agccactttg
    28621 ctcaattgaa acttctcctg aatcagtata aagtacatcc ttaccattac tagatttatt
    28681 tacatacgtt tcttcgctat tctcagaatt agttactttg ctcatagatg attcttcatg
    28741 ctctctaaat tcaattacag atacttttgc cgtactaaaa tgagtctctc catcactttt
    28801 agctttacta acaagaatat cagattctac tttgggagaa tataaatcag ttgatttact
    28861 acttattgaa aatccaattt tatcactttg ttcatttttt gttaaaccaa aagattcaac
    28921 ctcattaatc tctttatttt ctgttgcaaa aacaagacct gtcccacaaa ctaatacagt
    28981 tgaaactaaa aaagtaacat aagttgattt tttaaatttc ataatattaa ctattctctt
    29041 ttctattttt gtctttccaa atccactaca tattgtcatt gaaaatggat tattctcctc
    29101 tgctagactt attagtgtgg ttgcataatc agccctatat tcttcaccaa gtatactcat
    29161 aaccttttca tcacaactaa attctatgtc tttatttagt aaaaaaaaca tcacccaaac
    29221 taagggatta aaccaatgga caacaagagc tacaagcata aataacttta acagattatc
    29281 aaagctatta atatgaacca tttcatgagt taaaacaaag tctaaagttt gattattagc
    29341 ataattcatt tgctttggca aaataattcg tggttttaaa ataccagatg taagtggtgt
    29401 cctagtaaat tcagatatgc aaacaataaa tcttctaaaa cggctattct tacacatcca
    29461 ttttttaatg aactcatgat tttttatatg aattgaagtt ttaaaaagat taattatttt
    29521 aaaatatcta aagcataaaa gtatgctaaa cataatagta actataaaag acaaaatgcc
    29581 caacatttcc aacatcataa tattttcaat attagacatt atttcaaatt cgtcagaatt
    29641 gtttttacct gtagagtcac tagctttctt aagagtataa ttttctgttg atgtattacc
    29701 caattccaca tcaaaagtcg attcaattat actataaata ctaaaaccag aatttaaact
    29761 tactggtatt agaagtttaa ataaaacaat caaccacaat aatacaaata cctttttagg
    29821 taatttttta aataaacatg ctcttataaa aataactatt aaaagtaata ctccagaagt
    29881 aaaactcatt tctaacaatt taatgaacat aaatcttacc tcactttaat ttttcaacaa
    29941 tttgtttaag ttttgtaatc tcatcacttg aaagattttc tttattaata aaagctgaaa
    30001 gaaatttttc tgaagaccct ccaaacattt tattaattaa agtttctgtt tcctgttttt
    30061 gtacttcctc tttgcttatt aaagctttac aaacaaaatt cggctcaatt ctctctattg
    30121 caccttttga cactaatttt tttataacgg tatacgtagt gtttctattc catcctgttt
    30181 cttcttttaa aatttttact aactctccag ccttgctttc tcctctctcc catagaactt
    30241 ccattacttt aagttctgaa tcaaataatt ttatctccat ataaaatcgc tcctcaaata
    30301 tagttatcac aatagtcatt actataataa aaaactatca tagtagtcta tactatgtca
    30361 atcatttatt ttttaataat aaaaactgtc tttttaaaaa attcttataa aagacagttt
    30421 tatcaatgtt atactttaat ctttaattaa agccatattt tatttaaata tataactata
    30481 tatcttagta catagtataa gatttttctt tatctaatag tatatcctct acatctgggt
    30541 tttcttttac taattgtcta taaaaattac aattagtaac acctacatat ggaattagcc
    30601 atagtagacc tattccaaga gtagctattg aaagtatata ccatcctata aaactaaagt
    30661 ttagtacaaa taatttaagt acatttcctt tcatcatatc taaactagca cccattgatt
    30721 taaatacacc catattatcc ttgtcttcac aaattaagaa agtagcttga gaaacatata
    30781 atagcaatat ataaaccggt aacataagaa caagtattat aagaactaga gttattccag
    30841 tagaaataac agtccctatt ccaacattat cacttgataa atattcaatt gctccactag
    30901 taagtaaaac tccaaatgat aaaaacgctc ctataattgt gattataaaa gaataaatat
    30961 aaacaataat tgattcaaat aaataaaaac caaaacttct taaataaatt ctcccactta
    31021 caaagatatc tcctatcttg actttttcat ctttagtaat tttcaagaac atacatgtaa
    31081 atcctgctga aacgcaaaaa gctattacag ccactacaat agtagtcaat ggttcaaaag
    31141 gtaatcctac aattgatatt aatactatca atgcaatata tactgccata gaaattgcac
    31201 atatccccca gtttccagat aactgagctt tagatactcg ttttaattct tgccttgata
    31261 ccataaaaaa cacccctttc attttataaa aaaactttta aaaatataaa taatctttta
    31321 acgtataata ttaatatatc atattttcga ctatataaga tacaattatt caccatgttt
    31381 tatcaagata taccataatt gtattactag ttttatatag tcaacattac caaatacatc
    31441 aaataaattt gattaattat tcaacaagta ataaaataat ttcta
//
LOCUS       DAEPIN010000050        30411 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.050, whole genome shotgun sequence.
ACCESSION   DAEPIN010000050 DAEPIN010000000
VERSION     DAEPIN010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 30411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30411
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.050"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            complement(234..755)
                     /locus_tag="KR250_002462"
     CDS             complement(234..755)
                     /locus_tag="KR250_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBH0068391.1"
                     /translation="MKFKRLRTSENKLCYKAMELYKISFPFHEQRKSSLQEEILKNKE
                     YQFNLIYDKNQFVGIILCWETDDFIYVEHFCIFPEMRNNRYGQRALELLNKKGKTIIL
                     EIDLPIDEISIYRKAFYERVKYKTNNYEHIHPPYHEKFNGHNLIVMSYPEKLSKMEYD
                     KFNHYLRNNVMSF"
     gene            1111..1908
                     /locus_tag="KR250_002463"
     CDS             1111..1908
                     /locus_tag="KR250_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HBH0068392.1"
                     /translation="MEKREKRIIISSLLSVSILMGLVSIYSILNKEDIILTVKGQEQK
                     VSSFKKTVEELLDEQGIKYNSEDKINPSLDTELKDDMKIKVVKVTKSKKEEIEKIPFD
                     TKHVNDSNLLKGKSKVYQEGQEGEKKLVYNLTYHDGKLVKKVLSKEVISKEPTTKIIK
                     YGTKEKVLIASRGANIRGGKHMKVVATAYAGDTITSTGTTPRWGVIAVDPRVIPYGTK
                     VYIPKLGMTFVAEDCGGAIKGNRIDIFMNSEGKASNWGRKSIDIYLH"
     gene            2018..2608
                     /locus_tag="KR250_002464"
     CDS             2018..2608
                     /locus_tag="KR250_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HBH0068393.1"
                     /translation="MKIVCLGDSLTYGFGVSRSNSWTNIVNKETRLEIVNKGINGDTT
                     SGMLARFNEDVVKNSPDIVFIMGGTNDFIAGAGNEIINSNIMAMVHQSYSKNIIPIIG
                     IPLKPDIPNVREDWSCFTDFNIVSQNLESYSYWIKKFCMTFNTNFVDFYSEYNKNMEL
                     EGYKKLYFDGLHPTKEGHRIMADIFINSINKYIEEL"
     gene            2674..4371
                     /locus_tag="KR250_002465"
     CDS             2674..4371
                     /locus_tag="KR250_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HBH0068394.1"
                     /translation="MDYTKTYEEWIKGSYFDEDTKLELENIKNNEKEIEDRFYKDLEF
                     GTAGLRGIIEAGTNRINKYTVRRATFGLANYILENTTKEETSRGVVIAHDNRHKSRQF
                     CIESANTLAACGIKAYIFDSLRTTPELSFAVRSLNAIAGIVITASHNPPEYNGYKVYW
                     EDGAQVMPEIANAITEKVNSIHDYSTIPTLTEANKNLVVLLDESQDTKFIEAVKSQII
                     RKDLVKNVGKSFKIVYTPLCGTGNVPIRRALKEVGFENIIVVPEEENPDPNFAGLDYP
                     NPEEKKALNRGILLAKEKGADLVIATDPDCDRVGVAVKTTTGEYALLTGNQIGGMLTH
                     YIIEGLKENNKLKENPTIIKTIVTSEFGADIAKANNVDVLNVLTGFKFIGEKIKLFEQ
                     NKNRSYVFGYEESYGYLVGTHARDKDGVVSSLLISEMAAFYYSKGMSLYDGLIELYKK
                     YGFFKEQTISLTLKGIEGVEKIKEIISYFRENQIDYINSIKVVDKKDYKNGIDNLPKS
                     DVLKYFLEDESWVAIRPSGTEPKLKFYIAVKGASDIEADKKIQGLKKYIDDMVEKLK"
     gene            4585..5424
                     /locus_tag="KR250_002466"
     CDS             4585..5424
                     /locus_tag="KR250_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HBH0068395.1"
                     /translation="MFGIDKKLIEYIIAKSNYSELSSKSAEVSFFLMLSIFPFLIFTI
                     SSIAYIPILHLNKYIALFRNMMPEGAFAVLSSIIVSAIDNRNLKFLAVSFVLTMWTFS
                     RAVKALIKGMNRAYKVKETRSFFKILSISFLFTIMLLVLIFLSMIFLVYGEKIGYFIF
                     NLVGLDEIFIKIWDILRYTVGIITIIVIFTLLYKYTPNKKLTIKESAPGAIFATFAWF
                     LVSFLYSYYTNYYANYEVIYGSIAEIIVLMTWMYFSSWSIVIGYEVNSRLYFRKIRHE
                     MLK"
     gene            5941..7797
                     /locus_tag="KR250_002467"
     CDS             5941..7797
                     /locus_tag="KR250_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HBH0068396.1"
                     /translation="MDTHNKYVNFIKNIPVPFLYCRIVKRQEDIEYRVEYISKGMGKV
                     LQLEEGICDKNILDVLPVFKSKKYFKELFSNEVDCIKRYIPTLKNWINIKKQIIGDSY
                     IILYFGKIVFDYRQIIDSFDKKEKVAYIKDEEGIYIDCSENLIPILNNNIKTTKDIFG
                     KNDIEVWGENTGKLFRDDYREGVSSKKRFLQNLFEYEETFFMVEKYFLYDEDELLGTI
                     GIVDNIIYSGYSNRNYNSKDLMKMIEHSIPENMFYKDVYGNYIGFNSGFLNLACMNKE
                     ELLGKNSYKISEEEALIDKIFESDKGVVENKKVVTFELNISMNDENKCIEITKRPFFD
                     SYGSVIGIIGTARDISRRKRLEEEMDKTRMEFFANLSHELRTPINLISSSLQVIEKKE
                     ADLIESNDTLKRNLGIIKQNGNRILRLVNNVIDFTKMQSGYLDFKPEESDIIAFIEEI
                     CMSVADFASQNNIQLTFDTEIEEFSMLFDSEKLERIILNLLSNGIKYNKKDGKINIFL
                     YVKDNVFNMKISDSGIGIPKEKIDKIFNRFEQIDNELSYRVKGSGIGLSLVKSLVELH
                     EGSISLKSQLGIGSEFIVSLPVRSKNNIEKYNHKREISNELSKKLEIEFSDL"
     misc_binding    7880..8120
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    8186..8421
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            8506..9723
                     /locus_tag="KR250_002468"
     CDS             8506..9723
                     /locus_tag="KR250_002468"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HBH0068397.1"
                     /translation="MENKVKIGVLGYGVVGSGLIDIIDNNKEKRNIEIVGILVNNLEK
                     HKDKKYSNIITNNIDDIFNKDIDILVEVMGGLEPSLSYIKKALNNKIHVVTANKDLLA
                     ECGDELAKLASENKVSIKFEASVAGGIPVLKPIIESLEGNNIDSINAILNGTTNFILS
                     KMYDENLSYDMALRQAQELGFAEANPESDVLGYDAARKLSILSTLAYDNRVYWKDVYL
                     EGITDIDEKDIEYAKKLNCKIKLIGQSKYENDKVSAFVRPVLVEKDNILARIDNEFNA
                     VIVNGDSVGEVSFVGKGAGSLATGSAVYSDVIDIIDNRVSSIDSFTKDKIQVNKIVRE
                     KCGALLRFKKCNKDEILNIVENCLVKFDILNDDDELAIMVYADSEYEINNSLCLIKDK
                     GYCEKMNKMLKIS"
     gene            9840..10334
                     /gene="cdeM"
                     /locus_tag="KR250_002469"
     CDS             9840..10334
                     /gene="cdeM"
                     /locus_tag="KR250_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosporium morphogenetic protein CdeM"
                     /protein_id="HBH0068398.1"
                     /translation="MENKKYANGGYSEDWYERGESTAKWFQNDREEYERAAYDEDRER
                     RGSNCGCSDSGENRPRNCERFRREAEIREREAREAFCESSERKKEALAYECEARKLWE
                     EAEKYWDEYSKYNYKGIEYLAEAARLFDEGMECEARRNGNNGGNNNNCCHKCHKCNCN
                     CCRK"
     gene            10599..11864
                     /gene="hisD"
                     /locus_tag="KR250_002470"
     CDS             10599..11864
                     /gene="hisD"
                     /locus_tag="KR250_002470"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HBH0068399.1"
                     /translation="MKSIKTSSMKEKVDEKILREQVSSIIEDIRNNKDVALKKYNEKF
                     DRNTRDEFRITKEEIKEAYKHVDDEFINNLKIAAKNIREFAKAQKLSFENPFEKEIYP
                     GVILGQTNIPIESCLAYVPGGQYPLFSTALMLIIPAKVAGVKRIVACSPTMKNTEKIN
                     PKTLVAMDIAGADEIYATGGVQAIAAFTYGTEKINPVDIIVGPGNKFVTEAKRQCYGQ
                     VGIDFVAGPSEVLIIADETSNPVYIAADLLAQCEHDLNARGILLTNSLEVAQKVEKNI
                     ESMLKDLPTKDIAYSSWENNGEIILVDDMEEAIKISNFYAPEHLEVAVNEYNDICDRL
                     TNYGSLFIGNLSAEVFGDYVSGTNHTLPTLKASRYTGGVWVGTFIKTCTKQIFNEKAI
                     QSLAPVAEKLAKEEGLYAHAKAAEVRFKK"
     gene            complement(12067..12819)
                     /locus_tag="KR250_002471"
     CDS             complement(12067..12819)
                     /locus_tag="KR250_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBH0068400.1"
                     /translation="MSYFQYQGNSCFYKEYGQGKPIIFLHGNTGSSNMFKALVPLYVE
                     NFRCILIDFLGNGQSDRVSQFSPDIWHDEALQTIALIEHLNCGKVGLIGTSGGAWAAV
                     NTGLERPDLVEAIIADSFDGRTLNDNFIDNLITGREKSKQDIQARKFYEWCHGKDWEN
                     VVNLDTKALLQCANEKRPLFHKELCKLEMPILFTGSKEDEMCRHNLEEEYKQMATFIS
                     KASIHIFSQGGHPAILTNADEFAQLAKVFFKY"
     gene            complement(12937..13206)
                     /locus_tag="KR250_002472"
     CDS             complement(12937..13206)
                     /locus_tag="KR250_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0068401.1"
                     /translation="MSYQNSIDLLPKELIEQVQEYIDGKVIYIPKKQEHKKHWGENTN
                     TKQVLASRNSQICINFQNGMSIKQLSEKYFLTEKSIQRILKQQNL"
     gene            13681..14775
                     /locus_tag="KR250_002473"
     CDS             13681..14775
                     /locus_tag="KR250_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBH0068402.1"
                     /translation="MKNIVGKPQTIKKVNEDLIKNIIKNEGPITKPEISNITELSLAT
                     VNKIVEQLALKNEVKVSGLSESTGGRRAQLFEINANLEHIIALYYYRNCCIGVVANLL
                     GEIIYQEEFNIRMDTYENVNQDTFFVIDTLIKKEKGNNIRAIGIGVPGVVKKGVVKNI
                     PTIKSWEGINLENLIKSKYNTKVFIENDTNITTIGIYQKQYKDKYKNMALMYLEDGIG
                     SGIVINGELYLGSTNFAGELSYLNIEFGKYNSNNLNLEDYIMSLRKRYLESKDNKFKK
                     EILSIISNFIRTLVCVLNPQAMIIQCSILTKSDMEFIEKDVSNNVGEENFPKLIKVES
                     LREGSINGVISMCLKETDYQYSLSNKKGGY"
     gene            14777..15727
                     /locus_tag="KR250_002474"
     CDS             14777..15727
                     /locus_tag="KR250_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HBH0068403.1"
                     /translation="MKYLVSIDGGGTKTKFCVSDLDGNILKEHTTGSTNYKSVGIKKT
                     YENINNGFKKILKDLYIDYDDIEYTVFGISGCDSPNDYKIIMDEILKIGINKEKIYLA
                     NDAVLAFYAQADSPGLVIVAGTGSIILGIKEDGEIYRVGGWGYNFSDLGSGYDIGRKL
                     LKKVLLYCDECHEYSDLFSCVLDFFGANSFEQLAYMITDINNNVEIANLASLVIDCAE
                     QGDKLAIEILRESSTELSKLAQVILRKISNPMKVDKSEINIVLSGGTLNKTVYAQMLM
                     NNLKTENTDKNLKFISQENQPVYGGIRLAMALANRRVKHG"
     gene            15720..16904
                     /locus_tag="KR250_002475"
     CDS             15720..16904
                     /locus_tag="KR250_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0068404.1"
                     /translation="MDRKKVIVQIFTGGFKAKEIKFNDIKCKLLELLDCIDIEKVIMG
                     WSVDEELYVETKKLLNKYNVELYLWLPCFSEIGLLEKSNLLIDYMGKEVKGYALQEDE
                     NFEFYCPNNKENIKNIKKIYSTYFSDIKFDGIFLDKIRYGSFSNKLNGVFNCFCKDCL
                     NIYEKNNIDKESLMREMNNVSKGVIDNEDTPFGITEYINGKYKFKNKIWEDFFETKNQ
                     NIYKALLEISNYFKSKGLKIGIDTFSPFISYFTGQDLEKLQEVADFIKPMMYRTTKAP
                     AGLPFEFERFIDESIHKNKEIGKRNFLDILGIKEYEGDVYPIEFVLEEIDFMTRNLNK
                     DIYVGVEINKKEIISPVTPKYISENISNINKTTVKGYVLSWDLLSAPKENIDEIIKWF
                     RN"
     gene            16917..18413
                     /locus_tag="KR250_002476"
     CDS             16917..18413
                     /locus_tag="KR250_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HBH0068405.1"
                     /translation="MSKILEMQQIYKSFGKVDVLKGINFELEKGEVRALLGANGAGKS
                     TLIKILGGVHSQTKGKVILTGNEIMFKNPHHSKENGISIIYQELSLIPTLTVLENIFL
                     GREILKGSVFLNKKDMLEECMKICEDFDFNIDPHMVVSNLSVANQQMVEIMKAVSCNS
                     EIIIMDEPTTSLTNNEKDGLFKIISRLKSKGKTIVYISHILEEVFKVCDSASIMKNGI
                     MIGTYDVEDLTKLKITQLMTGNDNHKLIEKKDYTFAKYEEPPVLEVKNISWSNKIKNV
                     SFKVYRGEVVGLAGLVGSRRTEIINLIFGIDNKDSGDIFINGKKVDITSPKVAIKNKI
                     GLIPEDRKNLGLILEQAIYTNSSSVQIDKFKKFLFIDRNKEIEFSKKGVKELGIKVND
                     VKQEVKELSGGNQQKVVVSKWLEKDLDLIIYDEPTKGIDIAAKEDIFNTIKEFSSKGI
                     GVIFISSDLEEVIRVSDRILVTREGSIVDDLKNENIQVQDIMNSIFNV"
     gene            18436..19386
                     /locus_tag="KR250_002477"
     CDS             18436..19386
                     /locus_tag="KR250_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0068406.1"
                     /translation="MKKAFEKLNLRDYGVLLGFLALCIAISIATPSFLGKQNILNLLR
                     QSSIIGIISAGMTFVIISGNFDISVGAVAALAGAITMKFATGGTNLFASMLLGVAICA
                     VIGLINGIVVAKINVPSLIATMAMVTIVRGLLLMLTGGYPITENIPILDYIGNGYLFN
                     IPIPVVIFFIVVLASFIVLNKTKFGRYVYSVGGNQEASKLNGINVDSHKVKVFIINAV
                     LAGIAGIVLTGRLGTATAIAGEGYDMDAIASVVIGGTSVAGGSGSVLKTVIGVLLMSV
                     INNSFNLLGIDVYFQYIFKGLIILAAVGFDSYSKKKLASR"
     gene            19407..20369
                     /locus_tag="KR250_002478"
     CDS             19407..20369
                     /locus_tag="KR250_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBH0068407.1"
                     /translation="MKKLMKHLALLLSLVMIFGLVGCSNGGDKEKNGEKKIAVLLPGS
                     TGYFVATKQGIDAKSKELGVSVEYADAQWDASKQLSQAEDFMAKGVDMIALCGVDSAV
                     SERIVKAANDSDVPIVAFTNSIGSNPTGEFKGLVTYIGQNEEETGALTGKIAKNLLGE
                     TGGKAVLIEGVPGTTPQVLRKKGLEKELKDSNIEIVYNQTSNWEKEQALKVTEDLIQK
                     KTDFNVIICQDDNSATGAGQALKDAGLKDKVKVIGLGGSKDGLKAITDGLIDGTTYMS
                     AVEEGGLVIEKASKFLKGEKIEPVTQIKQVEVNKDNISEFKGEW"
     gene            20741..21373
                     /locus_tag="KR250_002479"
     CDS             20741..21373
                     /locus_tag="KR250_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBH0068408.1"
                     /translation="MARRLFLERIGLIFLGSAILAFGVYNFYYLNNITEGGVLGILLL
                     LKNLFNIQPAIANVVIDGLLLLVGYKFFGKKFLIYSIVASITFSVLYDLFEAIGPLVP
                     QSQNMLLSTILAGVTVGTGVGIVVKAGCASGGDDALALVISKTTSLNIGQVYLATDVI
                     VLLLSLFYLSAFDIFYSLIAVTISGKVIDFIYYHGKSLDMDISNDIVPEC"
     gene            21784..21876
                     /gene="kdpF"
                     /locus_tag="KR250_002480"
     CDS             21784..21876
                     /gene="kdpF"
                     /locus_tag="KR250_002480"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HBH0068409.1"
                     /translation="MEGLPMWFLAVVIIMLIIYLVYALINPEKF"
     gene            21917..23599
                     /gene="kdpA"
                     /locus_tag="KR250_002481"
     CDS             21917..23599
                     /gene="kdpA"
                     /locus_tag="KR250_002481"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015391009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HBH0068410.1"
                     /translation="MFMVQIVIVLAIFMILVIPMGKYLYHIATNQKTFGDRLFDKVDN
                     FIYKVCSIDKKKEMNWKQYALALLFTNAVMVFIGYIILRTQSMHIFNPSGIKSMEQGL
                     SFNTIISFMTNTNLQHYSGESGLSYFSQMTVIIYMMFTSAATGYAAAMAFVRGLVGKK
                     KTLGNFYVDLIRITTRVLLPGALIIGLILVTQGVPQTFAGTETVTTIEGKLQDIARGP
                     VAALESIKHLGTNGGGFFGSNSSHPFENPTIISNIVEILSMMILPGACVVAFGHMIKN
                     KKQGWVVFGAMSIIFLIGLVVCFKAESAGNPILSQLGLNQSMGSMEGKEVRFGIAQSS
                     LFTTVTTSFTTGTVNNMHDTLTPLGGLVPLLNMMLNVVFGGKGVGLMNMLMYAIIAVF
                     LCGLMVGRTPEFLTKKIEGKEMKLIALLIILHPLLILMFSGLSVAIPAGLEGISNPGF
                     HGLSQVLYEFASSAANNGSGFEGLGDNTMFWNITTGIVMFFGRYVSIIVLLAISSLLA
                     SKKAVNESIGTLRTDNFTFTIVLVLVVLIVGALTFFPALALGPISEHLVLWH"
     gene            23624..25681
                     /gene="kdpB"
                     /locus_tag="KR250_002482"
     CDS             23624..25681
                     /gene="kdpB"
                     /locus_tag="KR250_002482"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HBH0068411.1"
                     /translation="METKKTKFITKDIFKTSIIEAFKKLNPKYMMKNPVMFVVEIGFI
                     VTLILTIVPSLFGDEGNNLRAYNGIVTIILFITVLFANFAEAVAEGRGKAQADTLKKT
                     RKDTVARVIDTLGNEKMISASELKLGDIVLVNAGEVIPNDGEVIEGIASVDESAITGE
                     SAPVMRESGGDFASVTGGTTVVSDWLKIRITTKPGESFLDKMIALVEGASRQKTPNEI
                     ALNTLLVGLTLIFMVVLISLYPMANYAGVKIPISTMIALLVCLIPTTIGGLLSAIGIA
                     GMDRVTRFNVIAMSGKAVEACGDVDTMILDKTGTITYGNRLASEFIAVGNADSKDLIN
                     YSVMCSLKDDTPEGKSIVDLGMKLGSTGKTKEAEEAEFVEFSAQTRMSGINLADGRAI
                     RKGAYDSIIKRVKESGGTVPEDLEENVNRVAKLGGTPLVVCVDNEIYGIIYLKDTVKS
                     GLVERFARLREIGIKTIMCTGDNPLTAATIAREAGVDGFIAECKPEDKIDAIKKEQLE
                     GKIVAMTGDGTNDAPALAQADVGLAMNSGTTSAKEAANMVDLDSDPTKILEVVEIGKQ
                     LLITRGALTTFSIANDVAKYFAIIPAIFTLAIPQMEILNVMKLSTSFSAILSALIFNA
                     IIIPCLIPLAMKGVKYRPMKSQAMLMRNMLIFGLGGVIVPFLGIKLIDLLITPLVMVL
                     NLG"
     gene            25700..26326
                     /locus_tag="KR250_002483"
     CDS             25700..26326
                     /locus_tag="KR250_002483"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HBH0068412.1"
                     /translation="MKTLKPALLVSIVLLVVCGLVYPLVLTGVSQVAFKDKANGSMIE
                     VNGVKVGSELIGQSFTDARFFKGRVSSVNYNTYTKEDLVPDKDGNTSYGGVSSGSFNY
                     GATNPELHDRVQKDIEQFLKDNPTVKREDIPTDLLTASGSGLDPNISPASAKIQIPAI
                     AKASGISESKLQKIVEDNTSKKLFGVLGEDRVNVLKVNVEVAKILGLI"
     gene            26563..27471
                     /gene="cysK"
                     /locus_tag="KR250_002484"
     CDS             26563..27471
                     /gene="cysK"
                     /locus_tag="KR250_002484"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HBH0068413.1"
                     /translation="MLYNNALELIGNTPVVRLNNLGCTNGCSNIYVKLEKVNPAGSIK
                     DRAVYEMLEGLEQRGELKKGDVLVEATSGNTGIALSMIGKLKGYEVVIVMPETMSVER
                     RDLMKAYGANLVLTDGKAGMAGSIEKANELLKENPNYKSLKQFENKDNPNAHYKTTAV
                     EIMNDVKDLDIFVCGVGTGGTLIGTARYLKEQNPNIRVIALEPKKSPAISENKTGPHK
                     IQGIGANFVPENYDSSVVDEVILVDDEDAFDTVKLLAAKEGILVGISSGANIFGAIEM
                     AKKYPDKKIVTVAPDGVDKYMSMGIF"
     gene            27498..28088
                     /gene="cysE"
                     /locus_tag="KR250_002485"
     CDS             27498..28088
                     /gene="cysE"
                     /locus_tag="KR250_002485"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HBH0068414.1"
                     /translation="MFKKINKDIEYIMKNDPAARSKIEVFLLYPSVHAMIMHRMAHAL
                     YKKKKLFTARLISQISRFMTGIEIHPGAKMGEGILIDHGMGVVIGETAEVGNRVTIYQ
                     GATLGATGKDTGKRHPTVGDDVLIGAGTKILGPLNIGSNSKIGANSVVVKDVPNGATV
                     VGIPAKIVKIRNLEPVKKNKKEVSYEYDELDNVYYI"
     gene            28248..28436
                     /locus_tag="KR250_002486"
     CDS             28248..28436
                     /locus_tag="KR250_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HBH0068415.1"
                     /translation="MKANVNQDTCIGCGLCPSICPEVFDMKDDGKSHVIVDEVPSDAE
                     ESAAEARESCPVDAIDVH"
     gene            complement(28490..29359)
                     /locus_tag="KR250_002487"
     CDS             complement(28490..29359)
                     /locus_tag="KR250_002487"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HBH0068416.1"
                     /translation="MKLNRNDSCWCGSTKKYKKCHMDMDNKIDSFAKLGHIVPTHDII
                     KNESQIEEIKKSAVINTKILDYISDKIEIGMSTEDINKLVHDFTLQNGAIPAPLDYNG
                     FPKSVCTSVNNEVCHGIPSKDIILKDGDIINVDVSTIFNGYYSDASRMFMIGNVNENA
                     KKLVQVTKECVQKGLEVAKPWGFLGDIGDVINSYAKKNGYSVVREIGGHGVGLSFHED
                     PFVSYVSKKGTEMLLVPGMIFTIEPMVNEGTHEIFVDEENNWTIYTKDGKLSAQWECM
                     VLVTETGVEILTE"
     gene            complement(29556..>30411)
                     /locus_tag="KR250_002488"
     CDS             complement(29556..>30411)
                     /locus_tag="KR250_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0068417.1"
                     /translation="EDSIEYVFDVDISNDIKAKENPLINFNSLSLVVWSGETKHILQQ
                     MNILDLEDIHSYSIENNTNITIYENNTLQYKFDEESENIKDSSIDIIEQMLILPDDLN
                     YDKDEVENMKNRLAKINIKYLQTLKEKDIKIKLINSNLTDEPEFSDLKYQLPPCWVRS
                     GKTWKDVPGIYRNNSIVAKIGYSNPSYANVHSSKNLELHETAHAIDKNVLNKKSNSEE
                     FMEVFAQERYKLYDPKQVAHAYISKFIEEFFAESFVHYYLDEDSKNTLKENCPLTYDF
                     LEKLELNY"
BASE COUNT        11772 a         3221 c         5434 g         9984 t
ORIGIN      
        1 aagtttgtta tataaaatgt aattttgtct ataaaatctc acttgttttc ttaactttaa
       61 gcgtgaaaaa gagaatgtat catgaatttt ttagttcatg atgcatcctt ctttgtatga
      121 aatccaatat atctttatta tttaaacaat ggaaaagagc cttatttcta ttttgagaca
      181 tctcttttat ttaattatga agttttatac agttcaataa ataggaatta tatttagaaa
      241 ctcatgacat tatttcttag atagtgatta aatttatcat attccatttt agataacttt
      301 tctgggtaag acattacaat caggttatgc ccattaaatt tttcatggta tggtggatga
      361 atgtgttcat aattatttgt cttatatttt actctttcat agaacgcctt gcgataaatt
      421 gaaatctcat caattggaag gtcaatttct aaaataatag tttttccttt cttatttaaa
      481 agctctaacg ctctttgtcc atatctatta ttgcgcattt ctggaaaaat gcagaaatgt
      541 tctacataga taaagtcatc tgtttcccaa cataaaatta tgccgacaaa ctggttttta
      601 tcataaatta aattgaactg atattcttta tttttcaaaa tttcttcttg caaagaagat
      661 tttctttgtt catgaaatgg gaaactaatt ttatataatt ccatagcctt gtaacataat
      721 ttattttcac tggttcttaa ccgtttaaat ttcactttac tcctcctaat cacgaaaatt
      781 ccaatttatt tatgagttca ttttaccata gtcagtattt gcatacaatg tgttttaagt
      841 tgagtgtcat ttctttcttt tatttatgct tatatctcat agactaagag ataattgctt
      901 aagacataac taaaaactat tatattaagc tttgataatc taaaaacagt atatttatgg
      961 acttttctag gtctaaaagt aatcttaata attttagaag ttacaaaaaa gttacataaa
     1021 tttggaaagc tataatatac atatataata taaggttaaa actgtaatga ataatattaa
     1081 tttaaaattt gaacatggaa ggagattatt atggagaaaa gagaaaagag gataataata
     1141 tcatctttat taagtgtatc aattttaatg ggtttggtaa gtatatattc tatattaaat
     1201 aaagaagaca taattttaac tgttaagggt caagagcaaa aagtatcttc ctttaagaaa
     1261 acagttgagg aacttttgga tgaacagggt ataaagtata attctgaaga taagattaat
     1321 ccaagtttag atacagaact aaaagatgat atgaaaataa aagttgttaa ggtaactaaa
     1381 agtaaaaaag aagagattga aaaaattcca tttgatacaa agcatgtaaa tgatagtaat
     1441 ttgttaaaag gaaaatctaa agtttatcaa gaaggtcaag aaggagaaaa aaaactagtc
     1501 tataatttaa cttaccatga tggaaagtta gtcaaaaaag tcttatcaaa agaagtaata
     1561 tctaaggagc caactacaaa aattataaaa tatggaacta aagaaaaagt actaatagca
     1621 tcaagaggag caaatataag aggaggcaaa catatgaaag tggttgccac tgcatatgca
     1681 ggagatacaa taacatctac tggtacaact ccaagatggg gtgttattgc agttgaccca
     1741 cgtgtaatac catatggaac aaaagtatat atacctaaac tgggtatgac ttttgtagca
     1801 gaagattgtg gaggtgcaat taaaggtaat agaatagaca tttttatgaa cagtgaagga
     1861 aaagcttcaa attggggaag gaaaagtata gatatatatc tacattaaat tagtgaatag
     1921 aataacttta cttgaaggac aaaaaagtat aaaatatgaa ttaactaaac tgttagatta
     1981 ttcttaaaac ataaaaatat atctaagggg ggataagatg aaaatagtat gcttaggaga
     2041 cagtttaacg tatggatttg gagtatcacg aagtaattca tggacaaaca ttgtaaataa
     2101 agagactcga ttagaaatag taaataaggg tataaatggt gatacaacaa gtggtatgtt
     2161 agctaggttt aatgaagatg ttgtaaaaaa ttctcctgat atagttttta taatgggtgg
     2221 caccaatgat tttattgctg gtgcgggaaa tgaaattatt aattctaata taatggctat
     2281 ggtgcatcag tcatattcaa aaaatattat acctataata ggtataccat taaaaccaga
     2341 tattccaaat gttagggaag attggagttg ttttactgat tttaatatag tatctcaaaa
     2401 cttagaatca tatagttatt ggataaaaaa attttgtatg acatttaata ctaatttcgt
     2461 agatttttat tcagaatata ataaaaatat ggaactagaa ggatataaaa aattatattt
     2521 tgatgggtta catccaacca aagagggtca tagaataatg gcagacattt ttattaattc
     2581 aattaataag tatatagaag agttatagat tacattaaaa tgcttgttta tgcgtcagtt
     2641 gcaataaata catacagtag agaggggttt attatggatt atactaaaac ttatgaagag
     2701 tggataaagg gttcatattt tgatgaagat acaaagctag agttggagaa tataaaaaat
     2761 aatgaaaaag aaatagagga tagattctat aaggatttag aatttgggac tgcgggtctt
     2821 agaggaataa ttgaagcagg aacgaataga ataaataagt acacagttag aagagctact
     2881 tttggattgg caaactatat attagaaaac acaacaaaag aagaaacaag tagaggagta
     2941 gtaatagctc atgataatag acacaagtct agacaattct gtatagaatc tgcaaatact
     3001 ttggcagcat gtggtataaa agcatatata tttgatagtt tgagaactac tccagaatta
     3061 tcttttgctg ttagaagctt aaatgcaatc gctggtatag tcataacagc aagccataat
     3121 cctccagaat ataatggata taaggtatac tgggaagatg gagcacaagt tatgccagag
     3181 attgcaaatg caataacaga gaaagtaaat agtatacatg attatagtac aataccaacc
     3241 ttaacggaag ctaataaaaa tttggtagtt ttgttggacg aatcacaaga tactaaattt
     3301 atagaagcag tgaaaagcca aattataaga aaagatttag ttaaaaatgt aggtaaaagc
     3361 tttaagatag tttatacacc tctttgtgga acagggaatg ttccaataag aagagcattg
     3421 aaagaagttg gttttgaaaa tataattgta gttccagaag aagaaaatcc agacccaaat
     3481 tttgcagggc ttgattatcc aaatccagaa gagaaaaaag ctttaaatag aggtatatta
     3541 ctcgcaaaag aaaaaggagc agaccttgta atagcaactg acccagattg tgatagagta
     3601 ggtgtagcag taaaaactac tacaggtgag tatgctttac ttacaggaaa tcaaattggt
     3661 ggtatgctga cacattatat aatagaagga ttaaaagaaa ataataaatt aaaagaaaat
     3721 cctacaataa taaaaaccat agttacatca gaatttggag cagatatagc taaagcaaat
     3781 aatgttgatg tattaaatgt tcttacaggt tttaaattta ttggtgaaaa aataaaatta
     3841 tttgaacaaa ataaaaatag aagttatgta tttggatatg aagaaagtta tggttactta
     3901 gttgggactc atgctagaga taaagatggt gtagtttctt cattgttaat atctgaaatg
     3961 gcagcatttt actattcaaa aggtatgagt ttatatgatg gtttaataga actttataaa
     4021 aagtatggat ttttcaaaga acagactata tctttgactt taaaaggtat agaaggtgta
     4081 gaaaagataa aagaaataat atcttatttt agagaaaatc aaattgatta tataaatagt
     4141 attaaagtag ttgataaaaa agattataaa aatggtatag ataatcttcc taaatctgat
     4201 gttttaaaat actttttgga agatgaatct tgggtagcta taagaccatc tggaacagaa
     4261 ccaaaactta aattttacat agctgtaaaa ggagctagtg atattgaagc agataaaaag
     4321 attcaaggtt tgaaaaagta tatagatgac atggtcgaaa aattaaaata aaatattatt
     4381 attttataag ataattttgt aataatattt atttagctga aaattataat ttactttcat
     4441 tgagtagaat atggaaatgt tagtagaatt gttattttca gcttttattt gtgttatttt
     4501 tacttaaata attatattat agaatgaatc tgagtataat taatattgta taatgaaatt
     4561 aatttgatag aaaaggggat atttatgttt ggtatagata agaaattgat tgaatatata
     4621 attgcaaaat ccaattacag tgaattaagt tcaaagtcag cagaagtatc tttcttctta
     4681 atgttgtcca tatttccttt tttaatattt acaataagtt ctatagctta tataccgatt
     4741 cttcatttaa ataagtatat agctttgttt agaaatatga tgccagaagg tgcttttgct
     4801 gtgttatctt caatcatagt ttctgcaata gacaatagaa acctgaaatt tttagcagtc
     4861 agttttgtac ttactatgtg gacattttca agagcagtaa aagctcttat taaaggaatg
     4921 aatagagcct ataaagttaa agaaactagg tcttttttta aaattttatc tatatcattt
     4981 ttatttacaa taatgctttt ggtccttatt tttttatcta tgatattttt agtttacggt
     5041 gaaaaaatag gttattttat ttttaatctt gtaggattgg atgagatatt tataaaaata
     5101 tgggatattc taagatacac tgtaggtata ataacaataa tagtgatatt tacactttta
     5161 tataaataca ctccaaataa aaagttaact attaaagaat ctgcgccagg tgctattttt
     5221 gctacttttg cttggttttt agtatcattt ttatattctt attatacaaa ttactatgct
     5281 aactatgaag taatctatgg aagtatagct gaaataattg tacttatgac ctggatgtat
     5341 tttagtagtt ggtctatcgt tattggctat gaggtaaatt caagattgta ttttagaaag
     5401 ataagacatg aaatgttaaa gtaaagtatt taaaataaaa gagagtattt agtattataa
     5461 atgtaattga attaataaag aaatattgac atttaagtta aaaagtctag aatttttctg
     5521 ggcttttttt atttttagta taacttgaca aattttattt aatgggatag ttttaattta
     5581 taatcatctt taaaaagatg actattgata agtaagagat aaaattggta agtatcaaaa
     5641 aatatactaa ataaaaatta tagtcctaat gagttaattt ctgtattttc aaataatgca
     5701 gtatttagtc atgtgttttt atatgcgcct gtatttctaa ttagcaatat attagaataa
     5761 gctactgtaa ttagatttag aaaaaagcga cataaataat tataaacaaa taataaatta
     5821 cataaaatta aatttattca caagtatatc ttataaaata tgttataatt aatagaaaat
     5881 tgtcatataa ttaattaagt agtattatta taaataacat tgaaaggtag tggttagaat
     5941 atggataccc ataataaata tgtaaatttt ataaaaaata tacctgtacc ttttttatat
     6001 tgtagaattg taaagagaca agaagatata gaatatcgag tagaatatat aagtaagggt
     6061 atgggcaaag tattacaatt agaagaaggt atttgtgata aaaatatact agatgtattg
     6121 cctgtattta agtctaaaaa atatttcaaa gagttgttct caaatgaagt ggattgtata
     6181 aaaaggtaca ttccgacact taaaaactgg ataaatataa aaaaacagat aataggggat
     6241 tcatacataa ttttgtattt tggtaaaatt gtatttgatt atagacagat aatcgattcc
     6301 tttgataaaa aggaaaaagt agcttatatt aaagacgaag aaggcatata tatagattgt
     6361 agtgaaaatt taataccaat attaaataac aacattaaaa caacgaaaga tatctttggt
     6421 aaaaatgata tagaagtatg gggagagaat acaggaaaat tatttagaga tgattataga
     6481 gaaggagtat ctagtaaaaa aagattttta caaaatcttt ttgaatatga agaaacattt
     6541 tttatggttg aaaaatattt tttgtatgat gaagacgaac ttttaggtac tatagggata
     6601 gtagacaata taatatatag tggttacagt aatagaaatt ataactcaaa agatttaatg
     6661 aagatgatag aacactcaat tccagagaat atgttttata aagatgtgta tggaaattat
     6721 attggattta attcaggttt tttgaattta gcttgtatga ataaagaaga attattaggg
     6781 aagaatagtt ataaaatatc agaagaagaa gctttaatag ataagatatt tgaaagtgat
     6841 aagggtgtag ttgaaaataa aaaagtagtt acatttgagt taaatataag tatgaatgat
     6901 gaaaacaagt gtattgagat tacaaaaagg ccgttttttg atagctatgg aagtgttatt
     6961 ggaataattg gaacagcaag ggatataagt agaagaaaaa gattagaaga ggaaatggat
     7021 aaaactagga tggaattttt tgcaaattta tcccatgaac ttagaacgcc tataaattta
     7081 atatcatctt cattacaagt gatagaaaaa aaagaagcag atttaataga atcaaatgat
     7141 actttaaaaa gaaatctagg tataataaaa caaaatggaa atagaatatt gaggttagta
     7201 aataatgtta tagactttac aaaaatgcaa tcaggatatt tagattttaa accagaagaa
     7261 tctgatataa ttgcatttat agaggagata tgtatgtctg tagctgattt tgcaagccaa
     7321 aataatatac aacttacatt tgatacagaa atagaagaat tttcaatgtt atttgattca
     7381 gaaaaattgg aaagaataat attaaattta ctttctaatg gaattaagta caataagaaa
     7441 gatggaaaaa taaatatatt tttatatgtg aaagataatg tatttaatat gaaaatatct
     7501 gatagtggta taggtatacc aaaagaaaaa atagataaaa tatttaatag gtttgaacag
     7561 attgacaatg aactttctta cagagttaaa ggaagtggta ttggactttc attagtgaaa
     7621 tctttggttg agttgcatga gggaagtata tctttaaaaa gtcaacttgg tattggaagt
     7681 gaatttatag tttcattgcc tgttagaagt aaaaataata ttgaaaaata taatcataaa
     7741 agagaaatca gcaatgaact aagtaaaaaa ttagaaatag agttctcgga tttataagtt
     7801 taaatataaa aaataaaatt ttaaaaaagt attgacaatt gatttagaag ggtttaaaat
     7861 aaattacaac ataataaata aaaaggcgat gaataggaag agtaaatcta attcgattac
     7921 cagagagaag agtccgttgg ctggaagact ttttaaatta gagggattga aaactaccta
     7981 ggagctactt tctgaagtca tctgcattag tagattttag taagaatagt cgttagctgc
     8041 gttaaagtgt aaagtgagtt ttcatgacat atgaaaacta aattaggtgg aaccgcggga
     8101 ataatactct cgtccttatg ttataaggat gagagttttt tgttatttaa aaatataaac
     8161 agtaatcata ataattatag aaaatataaa agcaatgaat aggaagagta aatttaattc
     8221 gactatcaga gaggaaagtt cacaggctga aagactttct aagtttgaga aattgaaaac
     8281 tacctaggag ctgtggtctg aagttacttt aaaaagtttt agtaagacta accgttaact
     8341 gcgttaaagt gtaaagtgga ttttatcaat aaatgataaa atcaaattgg gtggaaccgc
     8401 gagtatagac tcgtcccttt atatagggat gagtctattt ttattgcaat aaaagattga
     8461 aaataaaaaa aaatcggaaa attataaatt taggaggaaa ataaaatgga gaataaagtg
     8521 aaaattggag tactaggata tggagttgta ggaagtggac ttatagatat aatagataat
     8581 aataaagaaa aaagaaatat agaaatagtt ggaattttag ttaataattt ggaaaaacat
     8641 aaggataaaa aatattctaa tataataact aataatatag atgatatatt taataaagat
     8701 atagatatac ttgttgaagt catgggtggt ttagaaccat cattatctta tataaaaaaa
     8761 gcattaaaca ataaaattca tgttgttaca gcaaataaag atttgttagc tgaatgtgga
     8821 gatgagcttg caaaattagc aagtgaaaat aaagtaagta ttaaatttga agcttcagtt
     8881 gcaggaggta taccagtatt aaaaccaata atagaatcac tagaaggaaa taatatagat
     8941 agtataaatg caattttaaa tggaactact aattttatat tatctaaaat gtatgatgag
     9001 aatctatctt atgatatggc attaagacaa gctcaagaat taggttttgc tgaagcaaat
     9061 ccagagtcag atgtattagg gtatgatgct gctagaaaac tatcaatatt atcaactcta
     9121 gcttatgaca atagagtata ttggaaggat gtgtatttag aaggaattac agatatagat
     9181 gaaaaagata ttgaatatgc aaaaaaatta aattgtaaaa taaaattgat tggtcaaagt
     9241 aagtatgaaa atgataaagt aagtgctttt gttagacctg ttttagtaga aaaagataat
     9301 atccttgcta gaatagacaa tgagttcaat gctgtcatag tgaatggaga ttcagtagga
     9361 gaagtttctt ttgtaggtaa aggcgctgga agtttagcaa caggaagtgc ggtttattca
     9421 gatgttatag atataataga taatagagtt tcaagtatag attcttttac taaagataaa
     9481 atacaagtta ataaaatagt tagagaaaag tgtggagcac ttcttagatt taaaaaatgt
     9541 aataaagatg aaattctaaa tatagttgag aattgtttag taaaattcga tattttaaat
     9601 gatgatgatg aacttgctat tatggtttat gctgattctg aatatgaaat aaacaacagt
     9661 ttatgtctaa taaaagataa aggatactgc gaaaaaatga ataaaatgtt gaaaataagt
     9721 taaaaaagaa aaaatataag tttatatgta acatattttg acgattttaa tactatatag
     9781 tatgagatga aacaaaacat ctcataaaaa aattaaaatt caagggagag atatgttata
     9841 tggaaaataa aaaatatgca aatggtggtt attcagaaga ctggtatgaa agaggagaat
     9901 ctacagctaa atggttccag aatgatagag aagaatatga aagagcagca tacgatgaag
     9961 atagagaaag aagaggctca aactgtggat gttcagattc aggagaaaat agacctagaa
    10021 actgtgaaag atttagaaga gaagcagaga taagagaaag agaagcaaga gaagcattct
    10081 gtgaatcttc agagagaaag aaagaagcac tagcatatga atgtgaagct agaaaattat
    10141 gggaagaagc agaaaaatac tgggatgaat attcaaaata caactataaa ggaatcgaat
    10201 atttagcaga agctgctaga ttatttgatg aaggtatgga atgtgaagct agaagaaatg
    10261 gaaataatgg aggaaacaat aataattgtt gccataaatg ccataaatgt aattgtaact
    10321 gctgtagaaa ataaaaaaat taattgacaa atcaatagtt aaaatatatc atattatgta
    10381 taataaagag aaaattcaga aaaaaataat gattagaaga aatataagta tatttattag
    10441 acaaatagtg ctcagttagt gaggtgagta caatatgatt aaactcccat cctctagctt
    10501 ttgatagagg gtgggagttt ttatttattt atatatctta aatgaggata atattgtaaa
    10561 ataaatgagt atgagattgt aaaattagga ggtaaaaaat gaagagtatt aaaacatcaa
    10621 gtatgaaaga aaaggtagat gaaaagatat taagagagca agtaagttct ataatagaag
    10681 atataagaaa taataaggat gttgcactta aaaaatataa tgagaagttt gatagaaata
    10741 caagagatga atttagaatt actaaggaag aaataaagga agcttataag catgtagatg
    10801 acgaatttat aaataactta aagattgctg ccaaaaatat aagagaattt gctaaagctc
    10861 aaaaactaag ttttgaaaac ccttttgaaa aagaaattta tccaggtgta atcttaggtc
    10921 aaactaatat tccaatagag tcatgtcttg catatgttcc aggaggtcag tatccattgt
    10981 tttcaacagc tcttatgctt ataatacctg caaaagtagc aggagtaaaa agaatagtgg
    11041 catgttcacc aactatgaaa aatactgaaa agataaatcc taagacattg gtagctatgg
    11101 atatagcagg tgctgatgaa atatatgcaa ctggaggagt tcaagctata gcagcattta
    11161 catatggaac agaaaagatt aatccagttg acataattgt aggaccagga aataagtttg
    11221 taactgaagc taaaagacaa tgttatggac aagtagggat tgattttgtc gctgggccaa
    11281 gtgaagttct tataatagcg gatgaaactt caaatccagt ttatattgca gctgatttat
    11341 tagcacaatg tgagcatgat ttaaatgcaa gaggaatatt attgacaaat agcttggaag
    11401 tagcacaaaa agttgaaaaa aatatagaaa gtatgctaaa agatttgcca acaaaggaca
    11461 tagcatatag ttcatgggaa aataatggag agataatatt ggttgatgat atggaagaag
    11521 ccattaaaat aagtaatttt tatgcaccag aacatttaga agttgcagta aatgagtata
    11581 atgatatttg tgatagatta actaactatg gttctttatt tattggaaat ctttctgctg
    11641 aggtgtttgg tgactatgtt tcaggtacca atcatacatt accaacactt aaagcatcaa
    11701 gatatactgg tggtgtttgg gttggaactt ttatcaaaac ttgtacaaag cagatattta
    11761 atgaaaaagc tatacaatca ttagcacctg tagctgaaaa gctggcaaaa gaagagggat
    11821 tatatgcaca tgcaaaggct gctgaggtta ggtttaaaaa gtagtaaaaa attttatttt
    11881 ggcagtcgag atttagtgtt agactgagat aaggaaaacg ctgagaaccc gaataaatac
    11941 tatattttgt gggcctcggc gtttctttta tatctgtata taataggata atactacctg
    12001 tgtgtttaaa tgctagctta atatttcttt tctaaataat gtttatgaaa tagttaatat
    12061 ttttagttaa tacttgaaaa aaacttttgc taactgtgca aattcatctg cattcgtcaa
    12121 tatagcaggg tgtcctccct gactaaaaat gtgaatactt gcttttgaaa taaaagttgc
    12181 catttgtttg tactcttctt caagattatg acgacacatt tcgtcttcct tacttcctgt
    12241 aaataaaatt ggcatttcta atttacacaa ttctttatga aacaaagggc gtttctcgtt
    12301 tgcacattgc aatagagctt ttgtatcaag attaacaaca ttctcccaat cttttccatg
    12361 acaccattca tagaattttc gtgcttgaat atcttgcttt gatttttccc ttccagtgat
    12421 tagattatct atgaagttat cattaagagt acgtccatca aagctatctg ctataattgc
    12481 ttcaactaaa tcgggacgtt ctaatccagt attaacagct gcccatgctc ctccacttgt
    12541 tccaattaag ccaactttac cacaattcaa atgttcaatc aatgctattg tctgtaaggc
    12601 ttcatcatgc catatgtcag gagaaaattg agaaactcta tccgactgtc cattacctaa
    12661 aaaatcaatt aagatacatc taaaattttc tacatataat ggcacaagtg ctttaaacat
    12721 attagaagaa ccagtattac catgcaggaa aattatcggt ttaccttgtc catattcttt
    12781 ataaaaacaa gaattacctt gatactgaaa atatgacata tattttcctc ctttttattt
    12841 gttaagttaa ttataacaat ataggtatga atgaaaaacc tcatgttctt tcttagaata
    12901 aaaatgtgcc acgcataaaa cgtgacacat ttctaattat aaattttgct gtttaagtat
    12961 tctttgtatg cttttctctg tcaaaaagta tttctctgat aactgcttga tactcattcc
    13021 attttggaaa tttatacaga tttgagaatt acgagaagct aagacttgtt tggtgtttgt
    13081 gttttcaccc caatgctttt tgtgctcttg ctttttggga atataaatca ctttaccatc
    13141 tatatattct tgcacttgct caatcagttc tttaggcagt aagtcaatcg aattttgata
    13201 gctcattttg cacctcctaa ataattattc tttaggatta gcaaaggcta ctgccactca
    13261 caattaaaaa taaaattttt tattatgtac aatagccttt gctatgcaca agttccaatt
    13321 cccataaact aaatactcct ttctggtttg aaaaattcat ctgaacattg atttcgcttt
    13381 taagatagag ttcaatgaca aatttacatt tttattatat atattcagat agataattgc
    13441 aactgtcttt gtatgaggat attttattat agtcaatact ataatatgta tttatatcat
    13501 caaactaata tgaacagttg aaaggaatat gaacaataaa acttagtcag gaagaataat
    13561 tatgaagcag acaatagaga aatttgattt taaagtttta ttgaaaattt attgacaaat
    13621 ttaacttgta aatattatac taaacttaat aaataaagtt tataaagtaa ggtgttgtct
    13681 atgaaaaata tagttggaaa accgcaaacg ataaaaaaag taaatgaaga tttaataaaa
    13741 aatattataa aaaatgaagg tcctataaca aaaccagaaa tttcaaacat tactgaattg
    13801 agtttagcta cagttaataa gattgtagag caattagctt tgaaaaatga agttaaagta
    13861 agtggactaa gtgaatctac tggaggaaga agagctcaat tatttgaaat aaatgcaaac
    13921 ttagaacaca taatagcttt atattattat agaaattgtt gtataggggt agttgccaat
    13981 ttattaggtg aaataattta tcaagaagaa tttaatataa gaatggatac atatgaaaac
    14041 gttaaccaag atactttttt tgttatagat actctaataa aaaaggaaaa aggaaacaat
    14101 ataagggcta ttggaattgg tgtcccagga gttgtaaaaa aaggtgtagt taaaaatata
    14161 ccaactataa aaagttggga aggtataaat ttagaaaatt taataaagag taaatacaat
    14221 actaaagtgt ttatagaaaa tgatacaaat attactacta ttgggattta tcaaaaacag
    14281 tataaagaca aatataaaaa tatggctctt atgtatttag aagatggtat tggttcaggt
    14341 atagttataa atggtgaatt gtatttaggt tctacaaatt ttgctggaga gttaagttat
    14401 ctaaatatag aatttggtaa gtataactct aacaacttaa atcttgaaga ttatattatg
    14461 agtttaagaa aaagatattt agaaagtaaa gataataaat ttaagaaaga aattttatct
    14521 ataatatcaa actttataag gactcttgta tgtgtcctaa atccacaagc tatgattata
    14581 cagtgtagta tattaaccaa gagtgacatg gagtttatag aaaaagatgt aagcaataat
    14641 gttggagaag aaaattttcc taaattaata aaagtagagt cattgagaga aggaagtata
    14701 aatggagtaa ttagtatgtg tttaaaagaa actgactatc aatattcctt atctaataaa
    14761 aaaggaggat attaatgtga aatatttggt ttcaatagat ggagggggaa caaaaactaa
    14821 attttgtgta agtgacttag atggaaacat acttaaagaa cataccactg gttcaactaa
    14881 ttataagtca gttggaatta aaaaaacata tgaaaatatt aataatggat ttaaaaaaat
    14941 attaaaagat ttatatatcg attatgacga tattgagtac actgtatttg gtatatctgg
    15001 ttgtgattct cctaatgatt ataagattat aatggatgaa atattaaaaa ttgggattaa
    15061 caaagaaaaa atatatctgg caaatgatgc agttttagct ttttatgcac aagcggattc
    15121 ccctggttta gtaattgtgg ctgggacagg ttcaattata ctgggaatta aagaagatgg
    15181 agaaatatat agagttggtg gatgggggta taattttagt gatttgggtt ctggatatga
    15241 tatagggaga aaattattaa agaaagtatt gctttattgt gatgaatgtc atgagtatag
    15301 tgatttattt agttgtgtct tggatttctt tggagcaaat agttttgaac aacttgcata
    15361 tatgattact gatataaaca ataatgttga aatagcaaac ttagcttcat tagtgattga
    15421 ttgtgccgaa caaggtgata aattggctat tgaaatatta agagaatcat caactgaact
    15481 atctaaatta gctcaagtaa tattgagaaa aatatctaat ccaatgaaag tggataaaag
    15541 cgaaataaat atagttttgt ctggtggtac attaaataaa actgtatatg ctcaaatgtt
    15601 aatgaataat ttaaaaacag aaaatacaga taaaaattta aaatttatat ctcaagaaaa
    15661 tcagccagtg tatgggggga taagacttgc tatggcatta gctaatagga gggtcaagca
    15721 tggatagaaa aaaggtaata gtacaaattt ttactggagg ttttaaggct aaagaaataa
    15781 agtttaatga tataaaatgt aaactactag aattattgga ctgtattgat atagaaaagg
    15841 ttatcatggg atggagtgta gatgaagaat tatatgtaga aactaaaaaa ctattaaata
    15901 aatataatgt tgaactatat ctttggttgc catgtttttc tgaaatagga ttacttgaaa
    15961 aaagtaattt gctaattgat tatatgggga aggaagtcaa gggctatgct ttacaagagg
    16021 atgaaaattt tgagttttat tgcccaaata ataaggaaaa tataaaaaat ataaagaaaa
    16081 tatatagtac ttattttagt gatattaaat ttgatggaat ttttttagat aaaataaggt
    16141 atggttcttt ttcgaataaa ctaaatggtg tttttaattg tttttgtaaa gattgtttaa
    16201 atatatatga aaaaaataat attgataaag agtcgttaat gagagaaatg aataatgtgt
    16261 ctaagggagt tatagataat gaagatactc catttggtat aacagaatac attaatggaa
    16321 aatacaaatt taaaaataaa atatgggaag atttttttga aacaaaaaat caaaatatat
    16381 acaaggcatt attagagata agtaattatt ttaaaagtaa gggattgaaa ataggtatag
    16441 atactttttc tccttttatt tcttacttta caggtcaaga tttagaaaaa ttacaagaag
    16501 tagcagattt tataaaacca atgatgtata gaactacaaa agctccagct ggtttaccat
    16561 ttgaatttga aaggtttatt gatgaatcca tacataagaa taaagagatt ggaaaaagaa
    16621 atttcttaga tattttagga attaaggagt atgaaggaga tgtatatcct atagaatttg
    16681 tactagagga aatcgatttt atgacaagaa atcttaataa agatatatat gtaggagttg
    16741 aaataaataa aaaggaaatt atatctcctg ttacacctaa gtacataagt gaaaatattt
    16801 caaatataaa taagacaaca gtaaaaggat atgttttatc ttgggatttg ttgtcagcac
    16861 caaaagaaaa tattgatgaa attataaaat ggtttcggaa ttagtgaggt gataaagtga
    16921 gtaagatatt ggaaatgcaa caaatatata aaagttttgg aaaggtagat gttttaaaag
    16981 ggataaattt tgagttagaa aagggagaag taagagctct tttgggagct aatggagcag
    17041 gaaaatctac acttattaaa attttaggag gagttcattc tcaaactaaa ggtaaagtaa
    17101 ttttgactgg taatgaaata atgtttaaaa atccacatca ttctaaagaa aatggtatta
    17161 gtataattta tcaagaatta agcttgatac caacgcttac agtacttgaa aatatttttc
    17221 ttggtagaga aattttaaaa ggaagtgtgt ttttaaacaa gaaagacatg cttgaggaat
    17281 gtatgaaaat ttgtgaggat tttgatttta atatagaccc acatatggta gtttctaatt
    17341 taagtgtagc aaaccaacaa atggttgaga taatgaaagc tgtttcatgt aattctgaaa
    17401 ttatcataat ggatgaacct acaacatcac ttactaataa tgaaaaagat gggctattta
    17461 aaataataag tagactaaag tctaaaggta aaacaatagt ctatatttct cacatattag
    17521 aggaagtttt taaagtatgt gatagtgcaa gtataatgaa aaatggaatt atgattggaa
    17581 cttatgatgt agaggattta actaaattaa agataactca acttatgaca ggaaatgata
    17641 atcacaaact tattgagaag aaggattata catttgcaaa atatgaagag ccaccagtac
    17701 tagaagtaaa gaatataagc tggtctaaca aaataaaaaa tgtttcattt aaagtttata
    17761 gaggcgaagt tgtaggtctt gcaggtcttg taggttcaag aagaactgaa attataaatt
    17821 tgatatttgg tatagataat aaagatagtg gagacatttt tatcaatggt aaaaaagtag
    17881 acataacttc tcctaaggtt gctataaaaa acaaaatagg attgattcct gaagatagaa
    17941 aaaatctagg attaatttta gaacaagcta tatacacaaa ctcatcatca gtacaaattg
    18001 ataaattcaa gaaattcttg tttatagata gaaataaaga aattgagttt tctaaaaaag
    18061 gtgttaagga attaggaata aaagttaatg atgtaaagca agaagtaaaa gagcttagtg
    18121 gtggaaatca acaaaaagta gtagtttcta agtggttaga gaaagattta gatttaataa
    18181 tatatgatga gccgacaaag ggaatagata ttgctgcaaa agaagatatt ttcaatacaa
    18241 tcaaagaatt ttcaagtaaa ggaataggag taatatttat atcttctgat ttagaagaag
    18301 taataagagt aagtgataga attttagtaa ctagagaagg ttctattgta gatgatttaa
    18361 aaaatgagaa tatacaggtt caagatataa tgaacagtat atttaatgta taaaaaatga
    18421 aaatggagga ctgaaatgaa gaaagctttt gagaagttaa atttaagaga ttatggagta
    18481 ttattaggtt ttttagcttt atgtatagct ataagtattg caactccatc atttttaggt
    18541 aaacaaaata tacttaatct tttacgacaa tcttctataa ttggaataat ttcagcaggt
    18601 atgacttttg ttataatatc tggaaacttt gatatatcag ttggagcagt agcagctctt
    18661 gcaggtgcta taacaatgaa atttgcgact ggtggaacta atttgtttgc atccatgctc
    18721 ttaggtgtag cgatttgtgc agtaatagga ttaatcaatg gtatagtagt tgcaaagata
    18781 aatgtaccat cattaatagc cactatggct atggtcacta ttgtaagagg attgctgttg
    18841 atgcttacag gaggatatcc aattactgaa aatataccaa tacttgatta cattggaaat
    18901 ggatatttgt ttaatatacc aataccagta gtaattttct ttatagtggt gttagcttca
    18961 tttattgtac ttaataaaac taagtttggt agatatgttt attctgtagg agggaatcaa
    19021 gaagcaagta aacttaatgg tataaatgta gattctcaca aagtgaaagt atttattatt
    19081 aatgctgtac ttgcaggaat agctggaatc gtactaacag gaagacttgg gactgcaact
    19141 gcgattgcag gtgaaggata tgatatggat gcaattgcat ctgtagtaat aggaggtaca
    19201 tcagtggcag gaggttctgg ttctgtactt aaaactgtta taggagtatt acttatgagt
    19261 gttataaaca acagttttaa tcttcttgga atagatgtat atttccagta catatttaaa
    19321 ggattaataa ttttagcagc agttggattt gattcttata gcaagaaaaa acttgcttca
    19381 agataaaaaa taagggggat ttttttatga aaaaattaat gaaacattta gcattgttgc
    19441 tatcacttgt tatgatattt ggtttagttg gttgttcaaa tggtggtgac aaagaaaaaa
    19501 atggagaaaa gaaaatagca gtattactac caggttcaac aggttacttt gtagcaacta
    19561 aacaaggtat agatgcaaaa tctaaagaat taggtgtaag cgtggaatat gcagatgctc
    19621 aatgggatgc tagtaaacaa ttatcacaag ctgaagattt tatggctaaa ggggtagaca
    19681 tgattgcatt atgtggagtt gattctgctg taagtgagag aatagttaaa gctgctaatg
    19741 attcagacgt tcctatagta gcatttacaa attctatagg aagtaaccca actggagaat
    19801 ttaaaggttt agttacatat ataggacaaa atgaggaaga aacaggagca ttaactggaa
    19861 aaattgctaa aaacttatta ggagaaactg gtgggaaagc tgtattaata gaaggagtac
    19921 caggaactac tccacaagta ttaagaaaaa aaggtcttga aaaagaatta aaagatagta
    19981 atatagaaat agtttataat caaacttcaa attgggagaa agagcaagct ttaaaggtaa
    20041 cagaagattt aattcaaaag aaaactgatt ttaatgttat aatatgtcaa gatgataatt
    20101 ctgctacagg tgcaggtcaa gctttaaaag atgctggtct taaggataaa gttaaagtca
    20161 taggtttggg tggaagtaaa gatggattaa aagctataac tgatgggtta atagatggta
    20221 caacttatat gtctgctgta gaagaaggtg gattagtcat agaaaaggca tctaaattcc
    20281 ttaaaggtga aaaaatagaa cctgttacac aaattaaaca agttgaagtt aacaaagata
    20341 atatttcaga gttcaagggt gaatggtaaa ttgtcataga taataaataa gatttttaga
    20401 gttactttag aaactatata aagtaactct ttttttaatt taatttagtg ataaattatt
    20461 gaatagtatt tctaaacaaa taaaaaatta atataaacaa agacttaatt atactaaatt
    20521 ataagtaaaa ctaatataaa cttatggaaa aaaggaaaac gtctaaagta ttggaattac
    20581 tatagtgtag gtgtggtatt aaaaaaacat aaaaaaacat aaaaaaattg ttgaaaaaaa
    20641 tatagaaatt ttgtataatt aaagatttga aaagtgcggt atacaaagcc aattcttttg
    20701 tatataatat atataaattc agaaaaagga agggatatgt atggccaggc ggttattttt
    20761 agaaaggatt ggacttatat ttttaggttc agcaatatta gcatttggag tttataattt
    20821 ttattattta aacaacataa cagaaggtgg agtgttaggt attttactac tgttaaaaaa
    20881 tttattcaat atacaaccag caatagcaaa tgttgtaata gatggtttgc tacttttagt
    20941 aggatataaa tttttcggta aaaaattcct tatatattca atagtagctt ctataacttt
    21001 ttcagtgtta tatgacttat ttgaagctat aggacctcta gtaccacaat cacaaaatat
    21061 gcttttatca actattttgg caggtgtaac tgttggaact ggtgtaggta tagtagtaaa
    21121 agcaggttgt gcatcaggag gagacgatgc tttagcatta gtaatttcta agactacatc
    21181 tttgaacata gggcaagttt atttagcaac agatgttatt gtattgttgt tatcattatt
    21241 ttatctatca gcgtttgata tcttttattc tttgattgct gttacaatta gtggcaaggt
    21301 aattgatttt atttattatc atggtaaaag tttggatatg gatataagta atgatatagt
    21361 tccagaatgt taatataaat taaataactt tagattttat taggggtggc agatttgcca
    21421 ccttttttgt gtctaaatct ttatagaaag tataatccta tctaggtttt tgcaaatctc
    21481 aaatatagaa ttatgttgaa atgactggga atacgattgc tagatgtgca ttttaaaaac
    21541 ccaattttat atttatgaaa tactaatcat taatggatgt ttcttaatcc tatctttata
    21601 cctaaaatga taagataaat gaagtagcaa tacaaaacta aaatttgtaa aagagattaa
    21661 attaaatgga ggagctaaaa tgttggatat agttatgata gcattgttat tcataggatt
    21721 tgtttccatg aagttacttg ctgattggtg tgggaaacaa actgaaaaaa aataatgtaa
    21781 tgtatggagg gattacctat gtggtttctg gcagttgtaa ttataatgtt aataatatat
    21841 ttggtatatg cgcttattaa tcctgaaaaa ttttaaacag tataaggcta ataagtaagt
    21901 tgaattgggg ggaacaattt ttatggtgca gatagtaata gtgttagcga tttttatgat
    21961 tttagtaata cctatgggca agtatttata tcatatagct actaatcaaa aaacatttgg
    22021 agatagacta tttgataaag tggacaattt tatatataag gtatgtagta tagataagaa
    22081 aaaagaaatg aactggaaac agtatgcatt agctttatta tttacaaatg cagttatggt
    22141 atttatagga tatatcatac ttagaactca aagtatgcat atttttaatc caagtggaat
    22201 taaaagtatg gaacaaggac tatcatttaa tacaataata agttttatga caaatacaaa
    22261 tcttcaacat tattcaggtg aatcaggact ttcatacttt agtcagatga cagtgataat
    22321 atatatgatg tttacatcag ctgcaacagg atatgcggct gctatggcat ttgtaagagg
    22381 tctagtcggt aaaaagaaaa cattgggaaa tttttatgta gatttaatta ggataacaac
    22441 aagagtactt ttaccaggag ctttaattat tgggcttata ttagttacac aaggagtacc
    22501 acaaactttt gctgggacag aaacagtgac tactatagaa ggaaagcttc aagatatagc
    22561 tagaggtcct gtggcagctc ttgaatcaat aaaacattta ggaacaaatg gtggtggatt
    22621 ctttggttca aattcatcac acccatttga aaatccaaca ataatatcaa atatagtaga
    22681 aatattatct atgatgatat taccaggagc ttgtgttgtt gcatttggtc acatgataaa
    22741 gaataaaaaa caaggttggg ttgtgtttgg cgcaatgtca atcatatttt taataggtct
    22801 agtagtgtgc tttaaagcag aaagtgcagg aaatcctatt ttatcacaat tagggcttaa
    22861 tcaaagtatg ggaagtatgg aaggtaagga agttagattt ggtatagcac aatcatcatt
    22921 attcacaaca gtgactactt cctttacgac aggtacagtt aataatatgc atgatacact
    22981 gactccttta ggaggattag taccactttt aaatatgatg ctaaatgtag tatttggtgg
    23041 taaaggcgta ggtctgatga atatgttgat gtacgctata atagcagtat tcttatgtgg
    23101 attaatggta ggaagaacac cagagttttt aactaagaaa atcgaaggaa aagaaatgaa
    23161 gcttattgct ttacttatta tattacatcc acttttaata ctaatgttct ctggtctttc
    23221 agtagcaata ccagcaggac ttgaaggaat ttcaaatcct ggattccatg gattatcaca
    23281 ggtattgtat gagtttgcat cttcagcagc caataatggt tcaggatttg aaggtctagg
    23341 agataacact atgttttgga atataacaac aggtatagtt atgttctttg gaagatatgt
    23401 gtctattata gtactactag caatatcaag tttgttagca tctaaaaaag ctgtaaatga
    23461 aagtatagga acacttagaa cggataattt tacatttaca atagttttag tacttgttgt
    23521 tttaattgtt ggtgctttaa catttttccc agccttagcg cttggaccta tttcggagca
    23581 tctagtgttg tggcactaaa aaattgggag ggagtaaagt tttatggaaa ctaaaaaaac
    23641 aaagtttatt acaaaagata ttttcaaaac ttccattata gaagctttta aaaaattaaa
    23701 tcctaaatat atgatgaaaa atccagttat gtttgttgta gaaattggat ttatagtaac
    23761 attgatactt acaatagtac ctagtttgtt tggtgatgaa ggcaataact taagagctta
    23821 taatgggatt gtaacaataa tcttatttat aacagtcctt tttgcaaatt ttgctgaagc
    23881 tgtagctgaa ggaagaggta aagctcaagc agatacactt aagaaaacta gaaaagatac
    23941 agtagcaagg gttatagata cgttaggaaa tgaaaaaatg ataagtgcaa gtgagctaaa
    24001 acttggtgat attgttttag taaatgctgg agaagttatc ccaaatgatg gagaagttat
    24061 agagggtata gcttctgtgg atgagtcagc aataactgga gaatctgcac ctgtcatgag
    24121 agaatcaggt ggagatttcg catctgttac aggtggtact acagttgtaa gtgactggtt
    24181 aaagataaga ataacaacaa aaccaggaga atcgttctta gataaaatga tagctcttgt
    24241 agaaggtgct tcaagacaaa aaacgccaaa tgagattgct ttaaatactt tgttagtagg
    24301 gttaacactt atatttatgg ttgttttaat atctctttat ccaatggcaa actatgcagg
    24361 tgtaaagatt ccaatttcta ctatgatagc tctcttggta tgtttaatac caactactat
    24421 aggtggattg ttgtcagcta ttggtattgc aggtatggat agagtaacta gatttaatgt
    24481 aattgcaatg tcaggaaaag cagtagaagc ttgtggagac gttgatacta tgattttgga
    24541 taagacaggt acgattactt atgggaatag acttgcatca gaatttatag cagttggaaa
    24601 tgcagattca aaagatttaa taaattattc tgttatgtgt tcattaaaag atgatacacc
    24661 agaaggaaaa tctatagttg atttaggtat gaaattaggt tcaacaggta agacaaaaga
    24721 ggcagaggaa gctgagtttg tagaattttc tgcacaaact agaatgagtg gtattaactt
    24781 agctgatggt agagctataa gaaaaggtgc ttatgatagc ataataaaaa gagtaaaaga
    24841 atctggtggt acagtgccag aagatttaga agaaaatgta aatcgagtag ctaaactagg
    24901 agggacacct ttagtagtgt gtgtagataa tgagatatat ggaattatct acttaaaaga
    24961 tacagtaaaa tcagggcttg tagaaagatt tgctagactt cgagagatag gaataaagac
    25021 aataatgtgt acaggggata atcccttgac agcagctaca attgcaagag aagctggagt
    25081 agatggattt attgcagaat gtaaaccaga agataaaata gatgcaataa aaaaagaaca
    25141 attagagggt aaaatagttg caatgacagg tgatggaaca aatgatgctc ctgcacttgc
    25201 tcaggcagat gtaggtcttg caatgaatag tggtacaaca tctgcaaaag aagcagcaaa
    25261 tatggtggat ttagattcag acccaactaa gatattggaa gttgttgaaa taggaaaaca
    25321 gcttttaata actcgtgggg ctttgacaac atttagtata gcaaatgatg ttgcaaaata
    25381 ttttgcgatt ataccagcta tatttactct tgcaatacct caaatggaga tattaaatgt
    25441 aatgaaactt tctacgtcat ttagtgcgat attatcagct ttaatattta atgctataat
    25501 tatcccatgt ttaatacctc ttgcaatgaa gggagtaaaa tatagaccta tgaaatcaca
    25561 agctatgtta atgagaaata tgcttatatt tggtcttggt ggagttatag taccattttt
    25621 aggaataaaa ttaattgatt tattgattac acctttagta atggttctta atttaggtta
    25681 ataaatggag gatataaaaa tgaaaacatt aaagccagca ttacttgtaa gtattgtatt
    25741 gttagttgtt tgtggacttg tatatccact tgttttgaca ggtgtaagcc aagttgcatt
    25801 taaagataaa gctaatggaa gtatgataga agtaaatggt gtaaaagtag gttcagaact
    25861 tattggtcaa agctttacag atgcgagatt ctttaaggga agagtatcat cagtaaacta
    25921 taatacttat acaaaagaag atttagtacc agataaagat ggaaacacat cttatggagg
    25981 tgtatcatca gggtctttta attatggagc tacaaatcca gaacttcatg atagagtaca
    26041 aaaagatata gaacagtttt taaaggataa tccaactgtc aaaagagagg atataccaac
    26101 agatttactt acagcatcag gttcaggact tgaccctaat ataagtccag cctctgcaaa
    26161 aattcagata ccagctatag caaaagcgtc tggaatatca gaaagtaaat tacaaaaaat
    26221 tgtagaagat aatacttcca aaaaactttt tggagtttta ggtgaagata gagttaatgt
    26281 attaaaggtt aatgtagagg tcgcaaaaat attaggtctt atatagtatt ttgtggaatt
    26341 ttagcaacaa aatattactc aagatttaat ttaaaggtta aattttaaag agtgaaaatc
    26401 gataatgatt ttcacttttt tatttgaata ttactaatat ttggaataaa gtgattgtaa
    26461 gtataaaatc caaaaaaagg ttgcattatc aaaatgataa tactataata tatttatcgt
    26521 tatcaatttg ataatagatt tggacatata ggaggaaata gtatgttata taataacgca
    26581 ttagagttga taggaaacac acctgtagta aggctgaata atttaggctg tactaatggg
    26641 tgttctaaca tatatgtaaa attagaaaaa gtaaatccag caggaagtat aaaagataga
    26701 gctgtttatg aaatgttaga gggtttagag caaagaggag aattaaaaaa gggagatgta
    26761 ctagttgaag caactagtgg aaatactggt attgcattat ctatgatagg aaaattaaaa
    26821 ggatatgaag tagtaatcgt aatgcccgaa acaatgagcg ttgagagaag agatttaatg
    26881 aaggcttatg gagcaaattt ggtgcttaca gatggtaaag ctggaatggc aggttccata
    26941 gaaaaagcaa atgaactttt aaaagaaaat ccaaactata aaagcttaaa acaatttgaa
    27001 aataaagata atccaaatgc acattataaa acaacagcag ttgaaattat gaatgatgtc
    27061 aaagatttag atatattcgt atgtggagtt ggaacaggtg gtactttaat aggaactgca
    27121 agatatttaa aagaacaaaa tccaaatata agagtaattg cacttgaacc aaaaaaatct
    27181 ccagctatat cagaaaacaa gactggacct cataaaatac aaggaatagg tgctaacttt
    27241 gttcctgaaa attatgattc tagtgtagtt gatgaagtaa tattagtaga tgatgaagat
    27301 gcatttgata cagttaaatt acttgcagca aaagagggaa ttttagtagg gatatcttct
    27361 ggagcaaata tatttggtgc tatagagatg gcaaaaaagt atccagataa aaagatagtt
    27421 acagtagctc cagatggagt tgataagtat atgtctatgg gaatatttta ataattttat
    27481 ttgagggagt gaatactttg tttaagaaaa taaataaaga tattgaatat ataatgaaga
    27541 atgatccagc agctaggtct aagatagaag tttttttact ttatccatca gttcatgcaa
    27601 tgataatgca taggatggct catgctcttt ataagaagaa gaagcttttt acagcaagat
    27661 taatatccca gatatccaga tttatgactg gcatagaaat acatcctggt gctaaaatgg
    27721 gggaaggtat attgatagac catggaatgg gtgttgtaat tggagagaca gctgaagtag
    27781 gtaatagggt gacaatatat caaggagcta ctttaggagc tacaggaaaa gatactggaa
    27841 agagacatcc aactgttggt gatgatgtat taattggtgc tggaacaaaa atactaggac
    27901 ctttaaatat aggttcaaat tcaaaaattg gtgcaaattc tgttgtcgtt aaggatgtac
    27961 ctaatggtgc aacagttgtt ggtataccag caaaaatagt aaaaataaga aacctagaac
    28021 ctgtaaaaaa gaataaaaaa gaagtgtcct atgagtatga tgaattagat aatgtatatt
    28081 atatatagga tttatataac tataaaataa actttataaa aattatttta aaaggtattt
    28141 ttttcaaatt tgaggacata ataaaaaaga actgaatgtt acagttttgg ttcatcaatc
    28201 tttgataaac aagattttaa atttgtagcg tctaggagga ttttattatg aaagcaaatg
    28261 ttaatcaaga tacttgtata ggttgtggat tatgtccatc aatatgtcca gaagtatttg
    28321 atatgaagga tgatgggaaa tcacatgtta tagttgatga agtaccaagt gatgctgaag
    28381 aatcagcagc agaagctaga gaaagctgtc cagttgatgc aatagatgtg cattaataaa
    28441 ataaatgcct ataaaaattg aattttcaat ttttataggc atttgtcttt tattcagtta
    28501 gtatttcaac acctgtttct gtaactaaaa ccatacattc ccattgagca gataatttac
    28561 catcttttgt ataaatagtc caattatttt cttcatcaac aaaaatttcg tgagttcctt
    28621 catttaccat aggttctatt gtgaatatca tcccaggaac caatagcatc tcagtccctt
    28681 ttttagatac ataacttaca aaagggtctt catgaaagct taatccaact ccatgaccac
    28741 ctatttctct aacaacagag tatccatttt tctttgcata agagttgata acatcaccta
    28801 tatctcctaa gaatccccat ggtttggcaa cttcaagacc tttttggaca cattcctttg
    28861 taacttgaac taattttttt gcattttcgt taacattacc tatcataaac attcttgaag
    28921 catctgaata gtatccgtta aaaatagtag aaacatctac atttataata tctccatctt
    28981 ttaagataat gtctttactt ggaataccat gacaaacttc attatttact gatgtacata
    29041 cacttttagg aaatccatta tagtctagtg gagcagggat agctccattt tgtagtgtaa
    29101 agtcatgaac caacttattt atatcttctg tagacattcc aatttcgatt ttatctgaaa
    29161 tataatccaa aatttttgtg tttattacag cacttttttt tatttcctca atttgacttt
    29221 cattttttat aatatcatga gttggtacaa tatgacctaa cttagcaaat gaatctattt
    29281 tattatccat atccatatga cattttttat attttttagt acttccacac caacagctat
    29341 catttctatt taacttcata acttttcatc cttcttttat tatttattta aattatgggt
    29401 tacataaact attttaaaac ataaccatta taaatattat aacataaata ctaagaaatc
    29461 ctttatagat agtgatttat tcaaagtata aattattata tggttattgt tgatgaaaag
    29521 aggatagtta aatgtattat tggatattag cgcacttaat agtttagttc aagtttttca
    29581 agaaaatcat atgttagagg acaattttcc tttaaagtat tttttgaatc ttcatcaaga
    29641 taataatgta caaatgattc tgcaaaaaat tcttctataa acttgcttat atatgcatgt
    29701 gcaacttgct tagggtcata aagcttatat ctttcctgag caaatacttc cataaattct
    29761 tcagaatttg attttttatt taaaacattt ttatctatag catgtgcagt ttcatgtaat
    29821 tctaaatttt ttgatgaatg tacatttgca tagctagggt tactataacc tattttagcc
    29881 actatagaat tatttctata tataccaggt acatctttcc aagtcttgcc tgatcttacc
    29941 caacatggag gtaattgata ttttaaatca ctaaattctg gttcatcagt tagatttgaa
    30001 tttattaatt ttatttttat atccttttct ttaagtgtct gtaaatattt aatatttatt
    30061 tttgcaagtc tgtttttcat attttcaact tcatctttat cataattcaa gtcatcagga
    30121 agtataagca tttgttcaat tatatctata ctactgtctt ttatattttc agattcttca
    30181 tcaaatttat attgaagagt attattttca tagatagtta tatttgtatt attttctata
    30241 gaataagagt gaatatcctc taaatctaaa atattcattt gttgtaaaat atgttttgtt
    30301 tcgccactcc acactactaa agaaagtgaa ttaaaattaa ttagtggatt ttcttttgct
    30361 tttatatcat tgcttatatc tacatcaaat acatattcaa ttgagtcttc t
//
LOCUS       DAEPIN010000051        30139 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.051, whole genome shotgun sequence.
ACCESSION   DAEPIN010000051 DAEPIN010000000
VERSION     DAEPIN010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 30139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30139
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.051"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            276..743
                     /locus_tag="KR250_002489"
     CDS             276..743
                     /locus_tag="KR250_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBH0068418.1"
                     /translation="MKFLNVLIVVEDIEKSKKFYYDVLGLKVICDFGENVVLEGNISL
                     QEKKLWLEFINKSDSEVKFNGNDAELYFEEDYFDTFVERLSTMRDIDYVHLAIEHRWG
                     QRAIRFYDLDGHIIEVGETMSSVCRRFLDSGLSIDEVAKRMDVTVEYIESVLE"
     gene            1910..2623
                     /locus_tag="KR250_002490"
     CDS             1910..2623
                     /locus_tag="KR250_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0068419.1"
                     /translation="MINIIICEGKKSERVILNDYINTFFYNYNFEYNIKEFENGGDLL
                     KKYPNNVDIIFMDIDLNDENGFNVSKEIKKFDESVRIIFVSSSNKYIKKGYEIGVYRY
                     LEKPLKYSLFKEAISSYLKECYKNEAILSIKCKCKIYFIKVDDILYLEVLKNDLTIHT
                     EKGIYTFKSSMKEIESNLEEHNFFRCHKSYLINLMKVNMLDIENNIVFINQNSIIVSR
                     YKIKLLREKLNSCLVDVII"
     gene            2896..4668
                     /locus_tag="KR250_002491"
     CDS             2896..4668
                     /locus_tag="KR250_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0068420.1"
                     /translation="MENGKFQFFKGVRNCFNMIYLHDKRYLFISMFMAIFQGIVPSIS
                     LLVSQNIINNMQTFSKSVKEILVLLIMYLIINIIPSIFQSFYGYYRIKFNKNFEKHIE
                     TMMLEKSLTLSLKDFEDDNTYDMINRAQSQKGFNITILSENLISIIRESVSTLSLIVV
                     LSQFQLWLIIVVIVIPVLKSLYSIKLGKEQYDVSVNRTWKERKAWYIDYLLSKGYAYK
                     EIMVFRIGSKLINHYKKLKEDIISQDMNIAKKNFIATLLCTVLDSIVSAGIIGFIIIQ
                     GILKKILIGDATTYIQAVQSIKDNVESIFSLFSSVIQDLFYTDLLFKFLDYNCKEDSI
                     EHIKIFEKIGDINKIELINISYKYNHSSEYSLKNINLTLNKNDPIAILGKNGSGKTTL
                     LKILLGFYDDYEGDILINGKNLKDIEKDEYIRKVSCMFQDYIKYEATLKENVAMGDVY
                     KSNLEEKVKSKLKETKLKPSIYEEDGLETILGTWFGKKQLSMGEWQKVAISRTLMKDA
                     DMYIFDEPDSSLDSEAGNELLSLYKKTFENKIGIFITHHIQHAKKITNNIVVLENGKL
                     LESGRHDELMDKKGVYFNLFCNCK"
     gene            4690..6111
                     /gene="ccpM"
                     /locus_tag="KR250_002492"
     CDS             4690..6111
                     /gene="ccpM"
                     /locus_tag="KR250_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-rich peptide radical SAM maturase CcpM"
                     /protein_id="HBH0068421.1"
                     /translation="MNQKPFIHTFKAGKSYFIYDVNTDKILKVNAAVYNYLNKIEHNL
                     EKESDWNIEIEDEINTLINYGFLKNKRVSKTMHPETKYIKYHIENNLSSIILQVTQNC
                     NLRCDYCIYSGSYSNRVHTNKRMNIDVAKRSIDYLIKNSSQLNEVSVGFYGGEPLLEF
                     DLIKQIVEYVKVVGEGKEIYFNLTTNATLFNEEIIEFFVKNNITTMISLDGGKEIHDK
                     SRKFANTGEGSHDVVLKNVRYIKNKYPEYYKKNITFNTVINSENDYISIDDFISKNET
                     LKDSNFLASTLNNNYSDKEIVSSDEYILGKSYDRFLTLLNKLKFIKDESMSPLSRIER
                     ALLDDFRYEKNIEGRLELPDVWHHGGPCIPGAFRIFVTTDGEFFPCERVSESSKMGMI
                     GNLDSGVDIEKAEKLLNIGKLYEEQCSDCWAYSYCDICIAKIDDTKCNENEIKPHDCD
                     RIRHSTENNMKDYCVLKSLGYQY"
     gene            6140..7210
                     /locus_tag="KR250_002493"
     CDS             6140..7210
                     /locus_tag="KR250_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04066 family peptide maturation system
                     protein"
                     /protein_id="HBH0068422.1"
                     /translation="MEKVLIYPFNYNFFTILNHCNKISDIEINSLVSPSGWGLCNKEY
                     NVNNIITKVTNDFQSELKKNSLVWFVDSKENLDLEKFIIPKLKVALKEGKKILYTRDD
                     LNKISRYISHYEFKKINEWNLEKYEIRDIEGISQINTPIIFICGLYGGLDTFDVNIRI
                     GEELRKRGFSILQFGPKKESYLFKMIPIPEFMFDDSISSKTKIINFNHFIKDKEVEAK
                     PDLIIIEVPGELFPFSRKMVGNFGIRAFEISNAVINDCGILCIPSSIYDREYCEELKY
                     FIKGRHGINIDYFSIEKKVIDTYETEVKQKFSYMSLDENFFVEDIEDYEKTFFIHDNK
                     QLLELVDNIVNQLKEYAKVISI"
     gene            7226..7477
                     /locus_tag="KR250_002494"
     CDS             7226..7477
                     /locus_tag="KR250_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide maturation system acyl carrier-related
                     protein"
                     /protein_id="HBH0068423.1"
                     /translation="MNYTEKELLLENIFFDVTGINFNKNNNLKKELFFSPSLHIQPRE
                     LLLVFCEVIENFNIKIPEEKLLNRQFTSFDNVLKIINMV"
     gene            7528..7731
                     /locus_tag="KR250_002495"
     CDS             7528..7731
                     /locus_tag="KR250_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0068424.1"
                     /translation="MSFGKRIEMNNIGVETRGVCTIMCPYTCKMMASSSCNCKNNDYN
                     ARGGSYNTYYNKLIHGQRTPDGM"
     gene            8217..8510
                     /locus_tag="KR250_002496"
     CDS             8217..8510
                     /locus_tag="KR250_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0068425.1"
                     /translation="MISMIMMLIGVLFIVFGIILTAVEIKGKMPGFYGVGQNAVRKNA
                     IGIIAAGIALIFFRNGLDSIDSILTYGVFLVIELVVIGLYFLIRIKYDKDGNL"
     gene            complement(8642..9991)
                     /locus_tag="KR250_002497"
     CDS             complement(8642..9991)
                     /locus_tag="KR250_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBH0068426.1"
                     /translation="MEKAKKLGLFSMILLGINSIIGSGIFLLPGKVYNLAGQNSMFIY
                     IFATLLVLSILLCFAEVGSMFDKKGGAYLYSKKAFGDFIGFEVGTMSWVIRIISWSTL
                     AVGFATALGSFWPESATEYKGYIAAILVTLLSINSLFGIKSTKIMNNVITIAKLVPLI
                     VFIIVGIFFIKFVNIVPSGNVVNSSMGPAIILVFYAFTGFESFIVASGEMENPKKNLP
                     VALITTIFICAIIYILIQIVCMGILGDRLFENSIPIADASSVFVGNYGKVFISVATLI
                     SIFGINIGSSIVTPKCGSSLAEEGSLPAFIGKTNKYGAPYVAIIISLICCIPLVLTGS
                     FEQLAVMSVIARFAQYIPTCLSVIVLRKRTDVKASFKIPFGPVIPIVAILGSLWLLQQ
                     AWAEDISKPITQNRVLIGLGAMLLIAPLYIFMKKNKEIEHKNKSEDSKLYISREKIQ"
     gene            complement(10028..11248)
                     /locus_tag="KR250_002498"
     CDS             complement(10028..11248)
                     /locus_tag="KR250_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBH0068427.1"
                     /translation="MQQALKLKYQTKELGKDFVIESCNSIKPWLINIRRELHKIPELA
                     LEENLTKQKVISYLKEIGIDYMEFTKHNGIMAYILKESADKTICIRADMDALPIEEEN
                     NIPYKSIHSGKMHACGHDAHTTMLLGACKVLHSIKDKLNVNVKFLFQPAEEGFGGAKF
                     LVEDGCLENPKADYIFGLHVMPHIETGLIETKYDTLNASVDTIKISIKGKRAHGAYPE
                     NGIDAIVTASQIVTSLQTIISRNLEPNNAVVLTIGKIYGGDAHNVICEDVKLEGTLRT
                     LNSKTRNFMIDKIAKIVGDTASAFGCVGTLHVSDENYPAVINEKELVDTVISNTKELL
                     GEEKFILRPNPSLGGEDFSFYTEHCKGAFFHLGCKNEEKGLISPLHTSSFNIDEDCLP
                     IGVMMHVMNTLYFN"
     gene            complement(11617..13629)
                     /locus_tag="KR250_002499"
     CDS             complement(11617..13629)
                     /locus_tag="KR250_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBH0068428.1"
                     /translation="MKKSVALVNDSRKDLIDFLENNLKLVFGDSININRYFINEINDN
                     DIINDDVILVMSVERLDKIINNILDKKKVIVVRRTFREDKIYNLLSLPQGTNVLIVND
                     SDETTLETISLFYKIGVTNIRPIPYMNDNNYKNIKIAITPGVPEKVPSFISDIFDLGH
                     RYIDISTFIEIINLLQIDSKEIQSNLVKYSEEIISLDTGIKDKYKELFLKIEELDTIL
                     NLSKDGILFTSKDGEINTYNSKVKDILDINEDIYGKYIEDIFVDSLKVLLSEKEILDK
                     VVVFNKKYINVNKKNIYNRDEKMGTYYSLQEITYIKKLEQNLTKKLREKGQIAKYTFK
                     DIKTNSPKMFECIDLAKKVSKSDLSILIRGESGTGKELIAQSIHNNSNRKNQPFIAVN
                     CAAVPENLLESQLFGYDKGTFTGGLKDGKQGLFELANNGTIFLDEIGDMPLELQTKLL
                     RVLQEKQIMPVGSHNVINIDVRIISATNKNLEQMIDNSQFREDLYYRLNTIPINIPPL
                     RERKEDILIIMEDLINKKLVITPEAKKLIQNYMWKGNIRELQNVTSYLNIMCEDIVLE
                     KDLPPNLRSSDNKNTSLKLKYSKNDILNILEILILNKESDVGIGRGLILKALLDKNLQ
                     ITEGKIKKIFEYLKKEELIICSSGRYGSKITQKGEDFYNKLKYKGL"
     gene            14100..14750
                     /locus_tag="KR250_002500"
     CDS             14100..14750
                     /locus_tag="KR250_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HBH0068429.1"
                     /translation="MKAEKTSIYPIIKNNLNKISIVEYKKGQRFVASDKDLQEVFFII
                     EGVALVECTTRGGNKFLVDIVPENEFIGKISYIYEHNLKCDIFAKTNIKLFRFEKNVF
                     EEFYLKPDFIALFHRKCTRRIYELYKTRMVRELFSCTEVIAYCISNKQENNVCNIKAI
                     RNFSEIYSISRKSRYHSLLKLTEKEIVKKMGNTYEILNYDELHKLAFEVKEFLEDE"
     gene            complement(14890..16266)
                     /locus_tag="KR250_002501"
     CDS             complement(14890..16266)
                     /locus_tag="KR250_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBH0068430.1"
                     /translation="MQELFSLKDENKRFYKILLSLCIPIIIQNLISTSVNVIDTIMIS
                     SLGETSVASVGVANQFFFLFNMSLSGITGGAGVFISQFYGKKDVSNIRKVTGLTCVLA
                     IVLSFVFVIPALLTPKPIIHIFSYDSEVVKLCIDYFSIAVFSYPLIAVSTVFSTGSRG
                     VRNPKLGMICSAFALVTNVILNYGFIFGNFGLPALGVKGAALATVIARICELILMITY
                     VYLYKKDYILKFGLKNLKAIDKIFIKSFSSKSFPIFVNDSVWAIGTVLYSVAYARAGT
                     SAIAASQIATSTGNFFIMTAVCIASGASIMLGNELGADHIKRAIEYAKKFSILVFSAG
                     LILGIILILNIPLLLKMFSVSDSLASDITKIFFIMGILMALKSFNTLVIIGILRSGGD
                     TKYALFLELGCMWLASIPLTFIAAFKGAPIFVLVLLTYSEEVVKFIFGVPRALSKKWA
                     INIVKEID"
     gene            complement(16282..18195)
                     /locus_tag="KR250_002502"
     CDS             complement(16282..18195)
                     /locus_tag="KR250_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBH0068431.1"
                     /translation="MIVLSCNNLNKSFGIDSILENISFTVNEGDKIGIIGVNGTGKTT
                     LFKIISGIYGYDSGDIYTSKDCEIGYLEQNTNFYSDNTILEEVLEVFKNLIEMESYLR
                     ELEVKISEESTKTNSPIIEKIMDEYSHKLELFSDLNGYGYKSEAKGVLKGLGFSDNDM
                     DKPISILSGGEKTRVLLGKLLLKKPTLLLLDEPTNHLDSEAIEWLEVFLKQYKGTVML
                     ISHDRYFLDQSVNRIFEVHNKKLKVYNGNYSKFVELSKIEKELELKKFEDQQKEIKKQ
                     EESIERLKAYGREKHLKRARSKEKALDKVDVLDKPEAYRKKARIQFTPSVQSGNDVLQ
                     IRDVSMGYGERILFKDLDLDIYRGEKVALIGANGVGKSTLFKIITNELQPLSGNIKFG
                     TNVHVSYFHQEQKTLNLDNTIIDEIWENNTHLTQTTLRNMLGAFLFVDEEVFKKISTL
                     SGGERARVAILKLILSNANLLLLDEPTNHLDIDSKEVLEEALTNYDGTIFTISHDRYF
                     LNTVVDKILVLDENGITEYLGNYDYYIDKKRQIQEMSIIEEKEEKTRTQIKDEKRKER
                     EQREIEKKNRIKRQNIEKEIEKLEIEIEKLDILLCQEEVYSNPDKAKEVSQEKINLEN
                     NLASLYDEWEEFM"
     gene            18331..18963
                     /locus_tag="KR250_002503"
     CDS             18331..18963
                     /locus_tag="KR250_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HBH0068432.1"
                     /translation="MLGNKNISMAVIRRLPKYHRYLGDLLDRDIQRISSKELSDIIGF
                     TASQIRQDLNNFGGFGQQGYGYNVEALHTEIGKILGLDRPYNAVLVGAGNLGQAIANY
                     AGFRKAGFEIKALFDANPRMIGLKIREFEVLDSDTLEDFIKNNNIDIAVLCIPKNGAQ
                     EVINRVVKAGIKGVWNFAPLDLEVPKGVIVENVNLTESLFTLSYLMKEGK"
     gene            19102..19641
                     /locus_tag="KR250_002504"
     CDS             19102..19641
                     /locus_tag="KR250_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HBH0068433.1"
                     /translation="MVFVEELWKKAIEFHGHECPGLAIGFKAALAAKKYLGCKQSGDE
                     EIVCISENDACGIDGIQVVLSCTMGKGNLLLRMTGKSVYHFFNRENGKSIRIYVKSKP
                     FEMDREGYKRYLLESDFEEIFEITDTKLRLPEKARIFKSLKCEVCGEFASEQYIRIEE
                     GKKVCLDCFDKYERFYEEY"
     gene            complement(19817..19987)
                     /locus_tag="KR250_002505"
     CDS             complement(19817..19987)
                     /locus_tag="KR250_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBH0068434.1"
                     /translation="MAYKITDACISCGACEAECPVSCISAGDDAYVIDAGSCIDCGSC
                     AGACPVDAPQPE"
     gene            20272..21339
                     /locus_tag="KR250_002506"
     CDS             20272..21339
                     /locus_tag="KR250_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HBH0068435.1"
                     /translation="MKNKLVYICIMSLLLFVVGCSNNPNKESTIKKDSKNNQSQVKEE
                     KREPVKKDITISFAGDVTMGNYKGSSYYGSFDHEFKRQGEDYNYFLKNVKPVFEKDDL
                     TVVNLEGPLTTASNAKEKKFAFKGKPGYVNILKSGSVEAVSIANNHSEDYFEEGMKDT
                     QFILDENKINYFGLGKDAVVDVKGIKVGLLGYNGLSTEYNEKNLKQMEDDIKSLKKKS
                     DVVVVYFHWGIELDYYPDKKQKDFAHYAIDKGADLVMGSHPHVIQGIEKYKNKYIAYS
                     LGNFCFGGNKNPSDTDSYIYQQTFTFSDNKLTSIKEPNIIPTSITSSNSRNNYQPKIL
                     DGSEKDRVLNKLEKISKDLNK"
     gene            21509..23410
                     /gene="cooS"
                     /locus_tag="KR250_002507"
     CDS             21509..23410
                     /gene="cooS"
                     /locus_tag="KR250_002507"
                     /EC_number="1.2.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic carbon-monoxide dehydrogenase
                     catalytic subunit"
                     /protein_id="HBH0068436.1"
                     /translation="MACNTCKMCESADKKLESFVASKDVETAFHRTEDQKVKCGFGLQ
                     GVCCRLCSNGPCRVTPKSPRGICGADADTIVARNFLRAVASGAACYLHVVENTAKNLK
                     NVGITKGVVKGEKTLNQLAEMFGIESDEKYDKCIKVADKVINDLYKSRDDKMELVEKI
                     AYAPRVKKWKELGIMPGGAKSEVFDAIVKSSTNLNSDPVDMLVNCLNLGISTGLYGLT
                     LTNLLNDVMLGEPVIRMAPVGFNVIDPDYINIMITGHQHSTFANFQDRLKDEDVIKLA
                     QSVGAKGFKLVGCTCVGQDLQLRGEHYQEVFAGHAGNNFTSEAVLSTGAIDIVLSEFN
                     CTIPGLEPIADKYKVKMVCLDDVAKKANAECIGLDRSKLDELSNTLIEKALESYKERR
                     GSIEIDIPKDHGFEQSLTGVSEKNLKAFLGDSWKPLINLIAEGKIKGVAAVVGCSNMT
                     AGGHDVNTVELTKELIKKDIIVLSAGCSTGGLENVGLMSPGAEELAGENLKEVCKTLG
                     IPPVLNFGPCLAIGRLEIVATELAAELGIDLPQLPLVLSAPQWLEEQALADGAFGLAL
                     GLPLHLALPPFITGGKLVTEVLTEKLKDLTGGHVIVNPDPKSSANQLEEIIIDRRKNL
                     GLKDVECNA"
     gene            23403..23876
                     /locus_tag="KR250_002508"
     CDS             23403..23876
                     /locus_tag="KR250_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBH0068437.1"
                     /translation="MHRIFINKDLCTGCKSCVLACMLKHNKDYDMYTLDLENIDNDSR
                     GHIELDSRHNNPVPILCRHCDEPECVLACMSGAMHKDSESGIVSYDEEKCGSCYMCVM
                     SCPYGLLKPDDRSKQNILKCDLCKDEEYPRCVANCPSGAIELQKEENDELCSVRG"
     gene            23851..25077
                     /locus_tag="KR250_002509"
     CDS             23851..25077
                     /locus_tag="KR250_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBH0068438.1"
                     /translation="MNYVVLGASAAGINAVKTLRELDKDSNIVVISKDENVYSRCMLH
                     HVISEHRTLKQINFVDEDFMEQNNVKWIAGKTVKGIDINKKVVQTEDITVNYDKLLIA
                     TGASSAIPPIKNLRDGNFVYSVRNIDDIYKIKEKAQNSKNVVIIGAGLVGIDALVGLF
                     KYEQLNISVAFMEKYILDRQLDEYTASVYENKFKEKGVKFYPSASIQEIVLDNSKNVT
                     GVAFSNGEVLDADMVIVATGVKPNADFLDGTGIEYDRGIIIDDMCQTTQKDIYAAGDV
                     VGKNAIWPLAVKQGIVAAYNMVGKDKKIEDEFAFKNSMNFMDIPTISIGMNTPVDDSY
                     KVLTRHGINDYKKFVFKDNVIYGAVIQGDISYVGVLTYLIKNKVEIYDLENRIFDICY
                     ADFFNIKENGEFCYSV"
     gene            complement(25216..26403)
                     /locus_tag="KR250_002510"
     CDS             complement(25216..26403)
                     /locus_tag="KR250_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBH0068439.1"
                     /translation="MNITKNFLKDYLEKFGETPFLVRLKDEEEFLIGEGSPQFKVIVN
                     NDISKSELLKSTSLALGEAYINRDIEVEGDLFLTLNLLLKQIDKFNIDNSAVKHLLHS
                     SNSMRHQKKEIHAHYDIGNDFYSLWLDKTLSYSCAYFRNDNDTLYDAQVNKVHHILTK
                     LNLEEGMSLLDIGCGWGFLLIEAAKKYKIHGVGITLSEEQFKEFNARIKEENLQDYLE
                     VRLMDYRELKKSDLMFDRVVSVGMLEHVGRENYELFIKNVNAVLKDGGLFLLHYISGL
                     QESYGDPWIKKYIFPGGVIPSLREMIHICSDYKYHTIDVESLRLHYVKTLLYWQKNFS
                     ENLPKIKEMFDDKFIRMWDLYLCSCAAAFNTGIIDIHQILFTKGIKNDLPLTREYMYN
                     CSK"
     gene            26676..27500
                     /locus_tag="KR250_002511"
     CDS             26676..27500
                     /locus_tag="KR250_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HBH0068440.1"
                     /translation="MEVYALVGSSGTGKSFKALEFAYENDIEYIIDDGILIYKNKVLA
                     GISAKQANTTIEAVKRAIFYNLEHRQEVREAIKKENIRKILIIGTSKKMVNQIVERLS
                     IGRVCKFINIQDISTKYEIEIAKQARKEGNHVIPVPAVEIKSMASGLSINSLKRLFRK
                     GNNRNMVVLEKTIIRPTFSYIGKFYISADVIKQIIEYEVYNFGSIDRINKINIENCNN
                     FMNIFVSININDLETIKKIEDIQQTIKKSIEKMTLVNVQKIDINIHKIKKINFLQC"
     gene            27629..28894
                     /gene="gluD"
                     /locus_tag="KR250_002512"
     CDS             27629..28894
                     /gene="gluD"
                     /locus_tag="KR250_002512"
                     /EC_number="1.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-specific glutamate dehydrogenase"
                     /protein_id="HBH0068441.1"
                     /translation="MSGKDVNVFEMAQSQVKNACDKLGMEPAVYELLKEPMRVIEVSI
                     PVKMDDGSIKTFKGFRSQHNDAVGPTKGGIRFHQNVSRDEVKALSIWMTFKCSVTGIP
                     YGGGKGGIIVDPSTLSQGELERLSRGYIDGIYKLIGEKVDVPAPDVNTNGQIMSWMVD
                     EYNKLTGQSSIGVITGKPVEFGGSLGRTAATGFGVAVTAREAAAKLGIDMKKAKIAVQ
                     GIGNVGSYTVLNCEKLGGTVVAMAEWCKSEGSYAIYNENGLDGQAMLDYMKEHGNLLN
                     FPGAKRISLEEFWASDVDIVIPAALENSITKEVAESIKAKLVCEAANGPTTPEADEVF
                     AERGIVLTPDILTNAGGVTVSYFEWVQNLYGYYWSEEEVEQKEEIAMVKAFESIWKIK
                     EEYNVTMREAAYMHSIKKVAEAMKLRGWY"
     gene            29107..29697
                     /locus_tag="KR250_002513"
     CDS             29107..29697
                     /locus_tag="KR250_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M56"
                     /protein_id="HBH0068442.1"
                     /translation="MHFKSKDLNLKYLVLFLKIVYWFNPFICIMEKYMDLDCELYCDE
                     RVLKNCTVQQKQEYALTIVNSMKRSTNFSNKFVAGLNKQSDIRKRVINMFSEKHKNGI
                     LRALIISLFSSITFFKFDSTSIIEENDFLERSRYTFEGRQEDMFVTIDFIYADVPIKL
                     RKEHEENCRVNGIVPKDSDSIEIIPRFYKDCMDLKK"
BASE COUNT        11493 a         3395 c         4550 g        10701 t
ORIGIN      
        1 tgaaattaaa aaaacataga tatttcagta atctaaaata gtctacaaaa ataacagttt
       61 tagcaaaatt aataagaata catatagatt taaacctaaa agtatgaatg tgtctgtttt
      121 ttacaagatt aaaatttata ttatatgata caattaactt aactatttca ataagactta
      181 taattttttt ctttatacta gaccttattg aaacctttat agtttaattc taattatata
      241 ttataaataa agtatgtgta tattaaggag gtaatatgaa gtttttaaat gttttaatag
      301 tagttgaaga tattgagaaa tcaaaaaagt tttattatga tgttttgggg ttaaaggtaa
      361 tctgtgattt tggagaaaat gttgtattag aagggaatat atcattacaa gaaaaaaagc
      421 tttggttaga gtttattaat aagagtgaca gtgaagttaa gttcaatgga aatgatgctg
      481 agctgtattt tgaggaagat tattttgata cttttgttga aagattgagt acaatgagag
      541 atatagatta tgttcatcta gctatagagc ataggtgggg tcaaagagca attagatttt
      601 atgatttaga tgggcatatt attgaggttg gagaaactat gtcatctgtg tgtagacgtt
      661 ttttagatag tggtctttca atagatgagg tagctaaaag aatggatgta actgtcgaat
      721 atattgaatc agttttagaa tagatggatt atattaaagt aaaactatat tttgtataga
      781 agtaagggct gtaagtaaac ttgtatggaa acaaatttac ttacagtttt tgtttttgta
      841 agatacagaa tataatattt gttataattt tatttggatt tttaaaaagt gcatgatttt
      901 ccttacatat tataatagaa tttaatattt attggatgat ttaaatttag aaaaatataa
      961 gtattagctt ataaaagata attatactta ctatcaagct gtagaatcat atatactatt
     1021 ttatgtaatt atgttaaaat ttaattgaac aaattaaaaa tatagtgaaa attgtagttt
     1081 tagtttattt atttaataaa tatttcaaat aaaataatgt ttaaataaga aacataaata
     1141 ataatattat tattgttctt aaagacaaaa aatcttagct taaaaatttg tatcatataa
     1201 aatccctaca tattatggaa gtaatgatac attatatgat atacacaata aagtataaaa
     1261 gtatttacat gttttttcta tatattttat aagttttagg tatggaaaat tactaaataa
     1321 aaattagatt aatgattatg atagtataga taaagtttat tctttttatt aatagaaatt
     1381 gttgtataaa aataataaaa gtgaggagag tgatatttaa ataaaaaaat tttaataagt
     1441 attggtaata tagtttttag ggatgattat tttatgtaaa aagttgttat ttgtaaagat
     1501 aaatatagaa aatattataa gtattgattt agtgataatc taaaaggtga gaattgtaat
     1561 aaaaagagaa catatccttc aaatggatgt aaacttataa ataacattat tacaagagat
     1621 ttattataca tatactgcaa ctattaatta gaatttatac tgtatttgta tttaaataaa
     1681 aacacaaata atattattgt aaaaatttat aattattaaa tatttaaagt ataagatata
     1741 ataattataa gtaatttatg tataaaatat agatatattt agattaaata ataattatta
     1801 ttattataca aaaattttgt attaaaaaat atctttaaat ttgaaatata ataaatgtta
     1861 gatagaattt tataaaattt aaaataaaaa attatataag gggtgtaata tgattaatat
     1921 tattatttgc gaaggtaaaa aatcagagag ggtaattcta aatgattata taaatacctt
     1981 tttttataat tacaattttg aatacaatat caaagagttt gaaaatggag gcgatttatt
     2041 aaaaaagtat cctaataatg ttgatatcat atttatggat atagacttaa atgatgaaaa
     2101 tggatttaat gtttcaaaag aaatcaaaaa atttgatgaa agtgtaagaa ttatatttgt
     2161 atcctcatca aataaataca taaagaaagg ttatgagata ggagtttata gatatttaga
     2221 gaagccctta aaatactctc tatttaaaga agcaatatca tcatatttaa aagaatgcta
     2281 caaaaatgaa gctattttat ctataaaatg taaatgcaag atatatttca taaaggtaga
     2341 tgatatttta tatttagaag ttttaaaaaa tgatttaact atacatactg aaaaaggaat
     2401 atatactttt aagagtagta tgaaagaaat agaatcaaat ctagaagaac ataatttttt
     2461 tagatgtcat aaaagctacc ttataaattt aatgaaggta aatatgctag atatagaaaa
     2521 taatattgta tttataaatc aaaattcaat aattgtaagt cgttataaga taaaattatt
     2581 gagagaaaag ttaaatagtt gtttggtaga tgtaataatt tgaggtaaat ttttgccttt
     2641 atattctatt ctaatagaat atatctattg taaaatatga aaaaatttat aataaaaact
     2701 aattatagtt aaaaatatac ctttcatcaa taacagattg aatatattaa gattttattt
     2761 tacaattcta ttaacaattt tattttaaag atttaattta aaatttaaat atgtattata
     2821 cataataaaa taatgttata ttatattaat taataaaata ttttatttca attttattaa
     2881 gtatggaggt gtattgtgga aaatggtaaa tttcaatttt ttaaaggtgt aagaaattgt
     2941 tttaatatga tttatctaca tgataaaaga tatttattta tctctatgtt tatggcaatt
     3001 tttcagggga ttgttccatc tatttctttg ttagtaagtc aaaatatcat taataatatg
     3061 caaacatttt cgaaaagtgt aaaagaaata ttagttttgt taattatgta tttaatcata
     3121 aatataattc cttcaatttt tcaaagtttt tatggatatt atagaataaa gtttaataaa
     3181 aattttgaaa aacatattga aacaatgatg ttagagaaat ctttaacttt gagtttaaaa
     3241 gattttgagg atgataatac ttatgacatg ataaatagag ctcaatctca aaaaggattt
     3301 aatattacta tactatctga aaaccttatt tcaataataa gagaatcagt atctacactt
     3361 tctcttattg ttgtattatc acaatttcaa ctttggctaa taatagttgt tattgttatt
     3421 cctgtattga aatcattata ttccattaaa ttaggaaagg aacaatatga tgttagtgta
     3481 aacagaactt ggaaagaaag aaaggcctgg tacatagatt atttgttgtc aaaaggttat
     3541 gcttacaaag agattatggt atttagaatt ggttctaaac ttattaatca ttataaaaaa
     3601 ttaaaagagg atattatatc tcaagatatg aatatagcta aaaagaattt tattgctacg
     3661 ctattatgta cagttttaga ttcaattgta tctgcaggaa ttataggatt tataataatt
     3721 caaggtattt taaagaagat tttaatagga gatgcgacta catatataca agcagttcaa
     3781 tctataaagg ataatgttga atcaatattt tcgttattta gttcagtaat acaagatctt
     3841 ttttatacag atttactgtt taaatttcta gattataact gtaaagaaga ctcaattgaa
     3901 catataaaaa tatttgaaaa aataggggat ataaataaaa tagagttaat aaatatttca
     3961 tataaataca atcattcatc agagtattct ttaaaaaata ttaatttaac tttaaataaa
     4021 aatgatccca ttgctatact aggaaaaaat ggttcaggta aaacgacact tttaaaaata
     4081 ttattaggtt tttatgatga ttatgaaggt gacatcttga taaatggaaa aaatttaaag
     4141 gatattgaaa aagatgaata cattagaaaa gtaagttgta tgtttcaaga ttatattaaa
     4201 tatgaggcta cattaaaaga aaatgtcgcc atgggagatg tatataagag taacttggaa
     4261 gaaaaagtta aaagtaaact taaagaaact aaattgaaac caagtattta tgaggaagat
     4321 ggattagaaa ctattttagg aacctggttt ggtaagaagc aactttctat gggtgaatgg
     4381 caaaaagtag caatatctag aactcttatg aaagatgctg atatgtacat ttttgatgaa
     4441 ccagattcat ctttagattc tgaagcggga aatgaactcc tttctttata taaaaaaaca
     4501 tttgaaaata agattggaat atttataacg caccatatcc aacatgcaaa aaagataact
     4561 aacaatattg tagttcttga aaatggaaag ttgttagaaa gtggaagaca tgatgaactt
     4621 atggacaaaa agggtgttta ttttaattta ttttgtaact gcaaataaat attaaaggga
     4681 ggttaattaa tgaatcaaaa accatttata cacacattta aagctggaaa atcatatttt
     4741 atttatgatg ttaatacgga taagatttta aaggtaaatg ctgctgttta taattatcta
     4801 aataaaattg agcataattt agaaaaagaa agtgactgga acatagaaat agaagacgaa
     4861 attaatacct tgattaatta tggatttcta aaaaataaaa gggtaagtaa aacaatgcac
     4921 cctgaaacta agtatataaa atatcatata gaaaataatc tatcctctat tattttacaa
     4981 gtaacccaaa attgtaattt acgttgtgac tattgcattt attctggttc atacagcaac
     5041 agagttcata caaataaaag aatgaatata gatgtagcaa aaagaagtat tgactatttg
     5101 attaagaatt ctagtcaatt aaatgaagtt agtgttggct tttatggagg agaaccctta
     5161 cttgagtttg atttgattaa gcagatagta gaatatgtaa aagtagttgg agaaggaaag
     5221 gaaatatact ttaatctaac tacaaatgca actttattta atgaggaaat aatagaattc
     5281 tttgtaaaaa ataatataac aactatgatt agtttagatg gaggaaaaga aattcatgat
     5341 aaaagtagaa aatttgcaaa tacaggagaa ggatctcatg atgttgtatt aaaaaatgta
     5401 cgttacataa aaaataaata tccagaatat tataaaaaga atattacatt taatacagtg
     5461 ataaattcag aaaatgatta catttctata gatgatttta tatctaaaaa tgagacttta
     5521 aaagattcaa actttttagc ttccactctt aataataact attctgataa agaaatagtt
     5581 tcatcagatg aatatatatt aggtaagagt tatgatagat tcttgacttt actcaacaaa
     5641 ttaaaattta taaaagatga gagtatgtca cctttatcaa gaatagaaag agccttatta
     5701 gatgatttta gatatgagaa aaatattgaa ggtagattag agttaccaga tgtgtggcat
     5761 catggtggac catgtatacc aggagctttt agaatatttg taactacaga tggagaattt
     5821 tttccatgtg agagagtaag tgaaagttct aaaatgggta tgattggaaa tttagatagt
     5881 ggagtagata tagaaaaggc tgaaaaactt ttaaatatag gaaaattata tgaagagcaa
     5941 tgttctgatt gttgggccta tagttattgt gatatatgta tagctaagat tgatgatact
     6001 aaatgtaatg aaaatgaaat aaaaccacat gattgtgaca gaattagaca ttctacagaa
     6061 aataatatga aagattactg cgttttgaaa tctctaggat atcaatatta aattttctaa
     6121 tgaaaggaaa gatgggatta tggaaaaagt acttatatat ccatttaatt ataatttttt
     6181 tactatttta aatcattgca ataaaatttc agatatagaa attaattcac tggttagtcc
     6241 ttctggctgg gggttatgca ataaggagta taatgttaat aatattatta ctaaagttac
     6301 aaatgatttt caaagtgaac ttaaaaagaa ttctttggta tggtttgttg attcaaaaga
     6361 aaatctagac ttagaaaaat ttataattcc taaattaaaa gtagctctaa aagaaggtaa
     6421 aaagatatta tatacacgtg atgatcttaa taaaatatct aggtatatat ctcattatga
     6481 attcaaaaag attaatgaat ggaatttaga aaaatatgaa ataagagata tagagggtat
     6541 atcacagata aacacaccaa taatatttat ttgtggtctt tatggagggt tagatacttt
     6601 tgatgtaaat ataaggatag gagaagaact aagaaaaagg gggttttcaa tattacaatt
     6661 tggccctaaa aaagagagct atttatttaa aatgattcct ataccagagt ttatgtttga
     6721 tgattcgata agtagtaaga caaagattat taattttaac cattttataa aagataaaga
     6781 ggtagaggca aagccagatt taataattat agaagttcca ggagaattat tccctttctc
     6841 tagaaaaatg gttggtaatt ttggaattag agcttttgaa atttctaatg cagtaataaa
     6901 tgattgtggt atattatgta ttccatcaag tatatatgat agagaatatt gtgaagaact
     6961 taaatatttt attaaaggaa gacatggtat aaatatagac tattttagta tagagaaaaa
     7021 agttatagat acatatgaga cagaggtgaa gcaaaaattt tcatatatgt cattagatga
     7081 aaactttttt gttgaagata tagaagatta tgaaaaaaca ttttttatac atgataataa
     7141 acaattacta gaattagtgg acaatatagt gaatcaatta aaagagtatg ctaaagtaat
     7201 atctatttag aattggaggt taattatgaa ttacactgaa aaggaattac tactagagaa
     7261 tatatttttt gatgtgactg gaattaattt taataaaaat aacaatttaa aaaaagaatt
     7321 atttttttca ccgtctttac atatacagcc aagagaatta cttttagtat tttgcgaggt
     7381 aatagaaaac tttaatataa aaattccaga ggaaaaactt ttgaataggc aatttacgag
     7441 ttttgataat gttctaaaaa taatcaatat ggtataggtt aataaaggta taaagtaagt
     7501 gatcttatag aaaaggggag gatatgaatg agttttggaa aaagaataga aatgaataat
     7561 ataggtgttg agacaagagg tgtttgtact attatgtgtc cttatacttg taagatgatg
     7621 gcatcttcat cttgtaactg caagaataat gattataatg caagaggagg atcatataat
     7681 acatattata ataaattgat acatggccaa agaactcctg atggaatgta aatatttcag
     7741 taataagtat actattaaat tgatttagca tatttgatta aatttatctt tcttacttta
     7801 tttcctttta ttataaaaat atgcagttga ttataattaa gagtgtgatt ttataataac
     7861 tgcataaata gtatctgtta tataaatcac ctcttcaatt attatagtgg taaatatagt
     7921 gattaggggt gatttttaca atttaaaagt ataaattaat taaaagttat tataataaat
     7981 tatacttatt taaatagtaa gaattaaaag taaagttttt aaatatattt gtaaaaatgt
     8041 atatatatat taataatatt gattagaatt aatatattat ttcaatattt aaacattatt
     8101 tattggtact aatttccaaa atagttaatt ggatatacaa tattattata aaaaataaat
     8161 attatttatt ttagatttta gaaattgaaa tttccttaag tgaaggaggt aattagatga
     8221 ttagtatgat tatgatgctt attggagtat tatttatagt gtttgggata attttaactg
     8281 ctgtcgaaat aaaaggaaaa atgccaggtt tttatggagt tgggcaaaat gcagtaagaa
     8341 aaaatgctat agggattata gcagcaggta tagcattaat tttttttaga aatgggttag
     8401 atagtattga ttcaattctt acctatggag tatttttagt gatagagtta gtagtaatag
     8461 gattatattt tcttataaga ataaaatatg ataaagatgg gaatctttaa agtataaatt
     8521 aataatattt tgattttgat atatgtttat ataaaaaaat tgtagataaa ttttataaaa
     8581 tagttttatc tacaattttt ttatatttat tatattttat gagaagtaat agtataaaat
     8641 attattgaat tttttctctc gaaatatata atttagaatc ttctgattta tttttatgct
     8701 cgatttcctt attttttttc ataaatatat atagaggagc tattaacaac attgccccta
     8761 atcctattaa aactctattt tgagttatag gttttgaaat atcttctgcc caagcttgtt
     8821 gtaacaacca taagctacct aaaatagcta ctattggtat aacagggcca aatggaatct
     8881 taaaagaggc cttaacatct gttctttttc ttaaaactat tactgataaa caagttggta
     8941 tgtactgagc aaatcttgca attacgctca ttactgcaag ttgttcaaaa cttccagtta
     9001 gcacaagtgg tatacaacaa attaaagata ttattattgc tacatatgga gcaccatatt
     9061 tatttgtttt tcctataaat gcaggaagag aaccttcttc tgctagagaa gaaccacatt
     9121 taggagttac tattgatgaa cctatattta taccaaatat agatattaat gttgctacag
     9181 atatgaaaac tttaccataa ttgcctacaa atacacttga agcatctgca attggaatac
     9241 tgttttcaaa tagtctatct ccaagtatac ccatacagac aatttgaatt aatatataaa
     9301 ttattgcaca tataaatatt gtggttatta atgcgactgg aagatttttt ttaggatttt
     9361 ccatttctcc acttgccaca ataaatgatt caaagccggt aaatgcgtaa aatactaata
     9421 taatggctgg acccatacta gagttaacta catttcctga aggaacaata tttacaaatt
     9481 ttataaaaaa tattccaact attataaaaa ctattaatgg tactaattta gctatagtta
     9541 ttacattatt cattatcttt gtgcttttaa ttccaaataa gctatttatt gaaagtagtg
     9601 ttacgagtat tgcagctata taacctttgt attcagtggc agattctggc caaaatgaac
     9661 ctaaagctgt tgcaaatcct actgctagag tagaccatga tataattcta ataacccaag
     9721 acattgtacc aacttcaaat cctataaaat ctccaaaagc ttttttggag tatagatatg
     9781 ctcctccttt cttatcaaac atgcttccaa cttctgcaaa acataaaagt attgatagaa
     9841 ctaagagtgt agcaaaaatg tatataaaca tactgttttg acctgctaga ttataaactt
     9901 tacctggtag taagaatata cctgaaccta ttatgctatt aatccctaat aatatcatac
     9961 taaatagacc taatttctta gctttttcca tattaattta tctcctattc ttttattaat
    10021 tactgtacta attaaaatac aaagtattca ttacatgcat cataacacct attggtaaac
    10081 aatcttcatc tatattaaag ctagaggtat gaagaggtga aattaagccc ttttcctcat
    10141 ttttacatcc taaatggaag aatgctcctt tacaatgttc tgtgtaaaaa gaaaagtctt
    10201 caccacctaa agacgggttt ggtctcaata taaatttttc ctctccaagt aattctttag
    10261 tatttgatat aactgtatct actaattctt tttcatttat aacagcagga tagttttcat
    10321 cacttacatg taaagtccca acacaaccaa atgcagatgc agtatctcca acaattttag
    10381 ctattttatc aatcataaag tttcttgttt tgctatttaa ggttcttaaa gtgccttcta
    10441 attttacatc ttcacatatt acattgtgtg catcaccacc atatatttta cctatagtca
    10501 aaacaacagc attatttggt tctaagtttc tacttattat agtttgtaat gatgtaacaa
    10561 tttgtgaagc tgtaactatt gcatcaatac cgttttcagg atatgcacca tgagctcttt
    10621 tacctttgat acttattttt atagtgtcta cactagcatt taatgtatca tattttgttt
    10681 cgataaggcc tgtttctata tgtggcataa catgaagtcc gaatatataa tcggccttgg
    10741 ggttttctaa acacccatcc tcaactaaaa attttgctcc tccaaaacct tcttctgctg
    10801 gttgaaataa gaattttaca tttacattaa gtttatcttt tattgaatgt aaaactttac
    10861 atgctccaag tagcatggtt gtatgtgcat catggccaca agcatgcatt tttccagaat
    10921 gtattgattt ataaggtatg ttgttttctt cttcaatagg aagagcatcc atatctgctc
    10981 ttatacaaat cgttttatct gcactttctt ttaaaatata tgccataatt ccattgtgtt
    11041 ttgtaaattc catataatca attccgattt cttttaaata agaaattacc ttctgtttag
    11101 taagattttc ttcaagagct aattctggaa ttttatgtaa ttctcttctt atgtttataa
    11161 gccatggttt tatagaatta caactttcta ttacaaaatc ttttcctaat tctttggttt
    11221 gatattttaa tttcaaagcc tgttgcattt ttattcctcc aattgctttt tattgtttta
    11281 ttaatataat aagcaattat tatgccataa ttttcgatgg agaattttgt tttttataag
    11341 aatattgaat aaatataatc atttatctat atgtatagta taaaaaatga ttgcatagtt
    11401 tcaattttca gtaaaaaata ttattttgaa gaaaatatat tttatatgct agtttatata
    11461 aaaaaaatag gaaataaaaa aattatccta ttcctattaa ttcctattat ttatgtacaa
    11521 aataaattat aattttaggg tacttgaaaa ataaaaaaac ctattgaagg agtactttaa
    11581 tcctgaatag atttctttgt ttgataaatt ataattttat agacctttat attttaattt
    11641 attgtaaaaa tcttctcctt tttgagttat tttacttcca tatcttcctg aactacaaat
    11701 tataagttct tcttttttta gatattcaaa tattttttta atttttcctt ctgtaatttg
    11761 aaggttctta tcgagtagtg cttttaaaat taatcctcta ccaataccaa catctgattc
    11821 cttatttaaa attagtatct ctaatatatt caatatatca ttcttactat attttagttt
    11881 taatgatgta tttttgttat ctgaagacct taagtttgga ggtaaatctt tctctaatac
    11941 aatatcttca cacataatgt ttaaatagga tgtaacattt tggagttctc ttatattacc
    12001 tttccacata tagttttgta taagtttttt tgcttctggt gtgattacta atttcttatt
    12061 aattaaatct tccataataa ttaatatgtc ttcttttctt tctcttagag gaggtatatt
    12121 tattggaatt gtatttaatc gataatataa atcctctcta aattggctat tatcaatcat
    12181 ttgttctaaa ttcttatttg tagcagatat gattctaaca tctatattaa ttacattgtg
    12241 agatccaaca ggcattatct gcttttcttg aagaactcta agtagttttg tttgtaattc
    12301 taaaggcata tcgcctattt catctaagaa tattgttcca ttattagcaa gttcaaataa
    12361 tccttgtttt ccatctttta gacctccagt aaaagttccc ttgtcatatc cgaatagctg
    12421 gctttctaat aaattttcag gaacggctgc acaatttact gctataaagg gttgattttt
    12481 tctatttgag ttgttatgta tagactgagc tatgagttct ttaccagttc cactttcacc
    12541 tctgataagt atgcttaaat cagatttaga aaccttctta gctaaatcta tacattcaaa
    12601 cattttagga ctatttgttt ttatatcctt aaatgtatat tttgctattt gtcctttttc
    12661 tcttaatttt ttagtgagat tttgttctaa tttttttatg tatgttattt cttgtaaact
    12721 ataatatgtg cccatttttt catctctatt gtatatgttt tttttattta catttatata
    12781 tttcttattg aatacgacaa ctttatctag tatctctttc tcactcaata aaacttttaa
    12841 actatctaca aatatatcct caatgtattt tccatatata tcctcattta tgtctaaaat
    12901 atcttttact ttactattat atgtatttat ttcaccatct tttgaagtaa ataatatacc
    12961 atcttttgat aaattcaaga ttgtatctaa ttcttctatt tttaaaaata attccttata
    13021 tttatctttt ataccagtat ctaaacttat tatttcttct gagtatttaa ctagattaga
    13081 ttgtatttcc tttgaatcaa tttgtaataa atttattatt tctataaatg tagagatatc
    13141 tatatatctg tgacctaaat caaatatatc tgagataaag ctaggtactt tttctggaac
    13201 tcctggagtt atagctattt ttatattttt atagttatta tcattcatat atggaattgg
    13261 tctaatatta gtcactccaa ttttataaaa taaacttata gtttctagag tagtttcatc
    13321 agaatcattt acgattagaa catttgtacc ctgtggtaaa gatagtagat tataaatttt
    13381 atcttcccta aaagttcttc ttaccacaat cacttttttc ttatctagta tattatttat
    13441 aattttgtct aatctttcta ctgacattac taatatcaca tcatcattta taatatcatt
    13501 atcatttatt tcatttatga agtatctatt aatatttata gaatctccaa aaactaattt
    13561 caaattattt tctaaaaagt ctattaaatc ttttctacta tcatttacca gagctactga
    13621 ttttttcatt tatttctcct tatataataa atagtttcca acaaaatagt tgtataaagc
    13681 aatctaattt tgataattta aatttatcaa ataaaaaaga aagtgtctat aatgactttc
    13741 taaaagttgc attaagacac tttctttctt atagttaaaa atcttgtcac caaatccccc
    13801 taattgttgc ttaaaactta aactgtatgt atatttttca tttctattcg attaaattat
    13861 aatatacacc caaaaaaatt tattttaata aattctattg ataatataat tttataaata
    13921 tagaattata ttaaaataaa ttatatatga aaatatatat gtataagttt attctgcgtg
    13981 agtagaatgg atttgtcaag tttaatttag ttgtaattct atcttttatt aaaaataaca
    14041 taaattaaaa catataaata aaatatttta tacaatatat tgtaaaggag gtaactttga
    14101 tgaaagcaga aaaaacaagt atatatccaa ttattaaaaa taatttaaac aaaatatcaa
    14161 ttgttgaata taaaaagggt caaagatttg tagcatcaga taaagactta caagaagtgt
    14221 tctttataat agagggagtt gcattagtgg agtgtactac tagaggagga aataaatttc
    14281 ttgttgacat tgtacctgaa aatgaattta taggcaaaat tagttacata tatgaacata
    14341 atttgaaatg tgatatattt gcaaagacta atataaaatt atttcgattt gaaaaaaatg
    14401 tatttgaaga gttttatctc aaaccagatt ttattgcact atttcacaga aaatgtacaa
    14461 gaagaatata tgaattatat aaaaccagaa tggtaagaga actattttct tgcacagaag
    14521 ttattgccta ttgtatatca aataaacaag aaaataatgt ctgcaatata aaagctattc
    14581 gtaatttcag tgaaatctat tcaattagta gaaaaagtag ataccattct ctgctaaagt
    14641 taactgaaaa ggagatagta aaaaaaatgg gtaatacata tgaaatttta aactatgatg
    14701 aattacataa attggcattt gaagtaaaag aatttttgga agatgagtaa aaattgttac
    14761 ttaatcaaaa taaagtataa taatttctgt aaaaaataga aatagtctca ctgaaaatca
    14821 aaaatacttt agttattttc aatgagactg tttagaaata ttgtatactt ataatgtata
    14881 gtataattat taatcaattt cctttactat attaatagcc cattttttag ataatgctct
    14941 aggaacacca aatataaact taaccacttc ttcactataa gtgagcaaaa cgagtacaaa
    15001 tataggtgca cctttaaatg cagctataaa agttagagga atagaagcta accacataca
    15061 tcctaattct aaaaatagag catatttagt atctcctcca cttcttaaaa taccaattat
    15121 aaccaatgta ttaaaagatt taagagccat tagtattccc attataaaga atatcttagt
    15181 tatatcagaa gctaagctat cagaaacact aaacattttt aataataatg gtatatttaa
    15241 aattaaaata attccaagta ttaaaccagc tgaaaataca agtatagaaa actttttagc
    15301 atattcaatg gctcttttaa tatggtcagc tccaagttca tttccaagca taatagatgc
    15361 accagatgca atacatacag ctgtcattat gaagaaatta cctgtactgg ttgctatctg
    15421 gctagctgct atagcagaag taccagctct tgcatatgca acagaataca ggacagttcc
    15481 aattgcccat acagagtcat ttacaaatat aggaaagctt tttgatgaaa aagattttat
    15541 aaaaatttta tcaatagctt ttagattttt tagaccaaac tttaatatat agtctttttt
    15601 ataaaggtaa acataagtaa tcattaaaat taattcacat attctagcaa taacagtagc
    15661 tagagcagca ccttttacac ccaatgcagg aagaccgaag ttaccaaata taaatccata
    15721 atttaaaata acatttgtta caagtgcaaa tgcactacaa atcataccta acttaggatt
    15781 tctaacacct cttgaaccag tactaaatac agtacttaca gcaattaatg gataactaaa
    15841 tactgcaata ctaaagtaat ctatacaaag ttttaccacc tcagaatcat acgaaaatat
    15901 gtgtataatt ggctttggtg ttagtaaagc aggaataaca aatacaaaac ttaaaacaat
    15961 tgccaataca caagtaagac ctgtgacttt tcttatatta ctcacatcct tctttccata
    16021 aaactgtgaa ataaaaactc cagcacctcc tgtgatacca gataaggaca tattaaaaag
    16081 aaagaaaaat tggtttgcaa ctccaactga agctacagat gtttccccca aactacttat
    16141 cataattgta tctattacat ttacagaagt agaaattaag ttctgtatga taataggtat
    16201 acataaagat agaagtattt tataaaatct tttattttcg tcttttaaag aaaatagttc
    16261 ttgcaaataa tcctctcctt tttacataaa ttcttcccac tcatcatata gagaggcaag
    16321 gttattttct aaattaattt tttcttggct aacttcttta gctttatctg gattagagta
    16381 tacttcttct tgacataaaa gtatatctaa tttttcaatt tcaatctcta acttttcaat
    16441 ttctttttct atattttgtc tttttattct atttttcttt tctatttctc tttgttctct
    16501 ctcttttctt ttttcatctt ttatttgagt tctagttttt tcttcttttt cttctataat
    16561 actcatctct tggatttgcc tttttttatc tatataataa tcataatttc ccagatattc
    16621 agtaattcca ttttcatcta aaactaaaat tttatcaaca acagtattta gaaagtatct
    16681 atcatgtgat attgtaaata tagtcccatc ataattagtt aaggcctctt caaggacttc
    16741 cttagaatca atatctaggt gattagtagg ctcatctaat aacaataagt ttgcatttga
    16801 tagtataagt ttaagtatgg caactctagc tctttctcca ccactcaatg tagaaatctt
    16861 cttaaaaact tcttcatcta caaataaaaa tgcccctaac atatttctca aagtagtttg
    16921 agtaagatga gtattatttt cccaaatctc atcaattatt gtgttatcta aattaagagt
    16981 tttttgttcc tgatggaagt aggaaacatg tacattagtt ccaaatttaa tattgccact
    17041 aagaggttgt agttcattag ttattatttt aaataatgta gatttgccaa caccatttgc
    17101 acctataagt gcaacttttt cccctctata tatatctaaa tccaagtctt taaaaagtat
    17161 tctttctcca taacccatag atacatctct aatttgaaga acatcattac cactctggac
    17221 agaaggcgta aattgtatac gagctttttt cctataagct tctggtttat ctaaaacatc
    17281 tactttgtct aatgcttttt ctttacttct tgcacgcttt aggtgttttt ctctaccata
    17341 agcttttagt ctttcaattg attcttcttg cttttttatt tctttttgtt ggtcttcaaa
    17401 tttcttaagc tctagttctt tttctatttt agataactca acaaatttag agtagttacc
    17461 attatatact ttaagctttt tattgtgaac ttcaaaaatt ctatttacgg attggtctaa
    17521 gaaatatcta tcatgagata ttaacatgac agtcccttta tattgtttta aaaatacttc
    17581 taaccattct attgcttcag agtctaagtg gttagtaggt tcatcaagta acaatagagt
    17641 tggtttctta agaagtaatt ttcctaaaag aactctagtt ttttcaccac cagaaagtat
    17701 gcttattggc ttatccatat cattgtcact aaatcctaat ccctttagaa ctcctttggc
    17761 ttcagattta tatccatatc catttaaatc tgaaaataat tctagtttat gtgaatattc
    17821 atccatgatt ttttctataa ttggagagtt tgtttttgta ctttcttcag atattttaac
    17881 ctccaattct cttaaatagg attccatttc tatcaaattt ttaaaaactt ctagtacttc
    17941 ttccaaaata gtattatccg aataaaaatt ggtattttgt tctaaatatc cgatttcaca
    18001 atctttagaa gtgtaaatat ctccactatc atatccataa attcctgaaa ttattttaaa
    18061 taaagtagtt tttccagtac cattaacacc gattatacca attttatcgc cttcatttac
    18121 tgtaaaacta atattttcta atatagaatc tataccaaaa cttttattta agttattaca
    18181 tgacaaaaca atcataaatt tctacctcct tgaactatct aaaattataa tgttttgata
    18241 taatataatc aaggttatat gtattagttt tgaaaaagaa atattttatt gtgaatcaaa
    18301 caaaaaagag gacactttta ggaggtgaag atgttgggaa ataaaaatat atcaatggca
    18361 gttataagaa ggctcccaaa atatcataga tatcttggag acttattaga tagggatata
    18421 caaagaatat cttctaaaga attgagtgat ataatagggt ttaccgcttc tcaaataaga
    18481 caagatttaa acaactttgg tggatttgga caacaaggat atggttataa tgtagaagct
    18541 cttcatactg agataggtaa aattcttggg ttggatcgac catacaacgc agttcttgta
    18601 ggagcaggta acttaggaca agctatagcc aattatgcag gatttagaaa agctggattc
    18661 gagataaaag ctttatttga tgcaaatcct agaatgatag gtttaaagat aagagagttt
    18721 gaagtattag attcagatac tttagaagac tttataaaaa acaataatat agatattgct
    18781 gtattatgta tacctaaaaa tggagcacaa gaagttatta atagagttgt aaaagctgga
    18841 atcaaaggtg tatggaattt tgcaccttta gatttagaag ttccgaaagg tgttatagtt
    18901 gaaaatgtaa acttaacaga aagtttattt accttatcgt atttaatgaa agaaggaaag
    18961 tagacaactt aaacatatta ataatgagca gttgtaaaga gtattgagaa agtcagaaga
    19021 tttaatatta aatcctctgg ctttttgaaa tattaggata aaaatttaaa taatgtatgg
    19081 aaataaacaa tattaaggag agtggttttt gtggaggaat tatggaaaaa ggcaattgaa
    19141 tttcatggtc atgaatgtcc agggttagct ataggattta aagcagcact tgcagcaaaa
    19201 aaatatttag gatgtaaaca gtcaggagat gaggaaattg tatgtatttc tgaaaatgat
    19261 gcttgtggaa tagatggtat acaagtagtg ctaagttgca caatgggtaa gggaaattta
    19321 ctacttagga tgactggaaa atcagtatat catttcttta atagagaaaa tggaaaatct
    19381 ataaggatat atgtaaagtc aaaaccattt gagatggata gagaaggata caaaagatat
    19441 ttattagaaa gtgattttga agaaatattt gagattacag atactaagtt aagacttcct
    19501 gaaaaagcaa gaatatttaa atcccttaaa tgtgaagtgt gtggtgaatt tgcatctgag
    19561 caatatataa ggattgaaga aggaaagaaa gtatgtcttg attgttttga taaatatgaa
    19621 agattttatg aagagtatta aaattataaa ttagattaaa gttaaaagta ttagatatgt
    19681 gctaacagta ttttaagatg aattaggtag caacataata aagtatataa ataatattaa
    19741 ttgaatcata cataattaaa aagaggctga ttagtattta taaaataaaa tcagtctctt
    19801 tatgaataac gaattattat tctggttgag gtgcatctac aggacaagct cctgcacaag
    19861 aaccacagtc tatacaagaa ccagcatcta taacatatgc gtcgtctcca gctgatatac
    19921 agctaacagg gcattcagcc tcacaagcac cacagcttat acaagcgtca gtaattttat
    19981 atgccatttt gaaatcctcc cttttatatg tattgtaatt caataacatt ataacaaata
    20041 ttattgaata taacaattaa attaattcta caacaaaaac tttattatga tattcttgtt
    20101 ttaaagtttt acaataaagt ttaatctgct tatattttta tttattttaa agaagtttat
    20161 tttaaaaata aaattaaatt taaaaagtta tgtattgatt atatttaaaa atgttatact
    20221 ctaaaaaagt atattttatt taaaaattta ttaatacaaa ggagatttac tatgaaaaat
    20281 aaattagttt acatatgtat tatgagtcta ttgctatttg tagtaggatg ttctaataat
    20341 ccaaataagg aaagtaccat taaaaaagac tctaagaata accaaagcca agttaaagaa
    20401 gaaaaaagag aacctgtgaa aaaagatata actataagtt tcgctggtga tgtaactatg
    20461 gggaactata aaggttcatc atattatggc agttttgacc atgaatttaa aagacaagga
    20521 gaagattaca attattttct taaaaatgta aaacctgtat ttgaaaaaga tgacttaaca
    20581 gtggttaatt tagaaggtcc acttactaca gcgtcaaatg caaaagaaaa aaaatttgca
    20641 tttaaaggaa agcctggata tgtaaatatt ttaaaatctg gaagtgtaga ggcagtaagt
    20701 attgcaaata atcattcaga agattatttt gaagaaggaa tgaaagatac acagtttata
    20761 ttggacgaga ataaaataaa ttattttgga ttagggaaag atgctgttgt agatgtcaaa
    20821 ggaattaaag tgggtctatt agggtataat ggattatcaa ctgaatataa tgaaaaaaat
    20881 ttaaaacaaa tggaagatga tattaagtcc ttaaaaaaga aatcggatgt tgttgtagtg
    20941 tactttcatt gggggataga attagattat tatccagata aaaaacaaaa agattttgct
    21001 cattatgcta tagacaaggg agcagattta gttatggggt ctcacccaca tgtgattcaa
    21061 ggaatagaaa agtataaaaa taagtatata gcttatagtc tagggaattt ttgctttgga
    21121 ggaaataaga atccttctga tacagatagt tatatatatc aacaaacctt tactttttca
    21181 gataataaat taacttcaat taaagagcca aatataatac caacttcaat aacatctagt
    21241 aactccagaa ataattatca accaaaaata ttggatggaa gtgagaagga tagagtttta
    21301 aataaacttg aaaaaataag caaagattta aataaataaa atgtaaaaaa aaataaatat
    21361 atatatttat tttaataatt tatattttgt ttagggtatg gtaaatcgtg gtattaaata
    21421 agtagatttt aatagttaaa ttatagtcaa ttataataaa gctatttaat ctactttttt
    21481 aatataaaaa tataaggggg aatttgtaat ggcttgtaac acttgtaaaa tgtgcgaaag
    21541 tgcagacaag aaattagaaa gttttgtagc ttcaaaggat gttgaaacag catttcatag
    21601 aacagaagat cagaaagtta agtgtggttt tggacttcag ggggtttgtt gtagactctg
    21661 ttcaaatgga ccatgtagag taactcctaa atcaccaaga ggtatatgtg gagcagatgc
    21721 agacactata gtagcaagaa actttttaag agcagtagca tcaggagcag cttgttattt
    21781 acatgtggta gaaaatacag ctaagaattt aaaaaatgtt ggaataacta aaggtgttgt
    21841 taaaggagaa aaaacattaa atcaattagc agagatgttt ggaatagaaa gtgatgaaaa
    21901 gtatgataaa tgtataaagg ttgcagacaa agttatcaac gatttgtata aatcaagaga
    21961 tgataaaatg gaattagtag aaaaaatagc ttatgcacct agagtgaaaa agtggaaaga
    22021 gttaggtata atgcctggtg gtgcaaagtc agaagttttt gatgcaatag taaaatcatc
    22081 aacaaattta aacagtgacc cagtagacat gctagttaat tgtttgaact taggtatatc
    22141 aacaggatta tatggtctta cactaactaa tcttttaaat gatgttatgt tgggagaacc
    22201 agttataaga atggctcctg taggatttaa tgtaatagac ccagactaca taaatataat
    22261 gataacaggt catcaacatt caacatttgc aaatttccaa gatagattaa aagatgaaga
    22321 tgttataaaa ttagctcaat ctgttggcgc aaaaggattt aagttagtag gatgtacatg
    22381 tgttggtcaa gatttacaat taagaggaga acattatcaa gaagtatttg ctggacatgc
    22441 aggtaacaat tttacaagtg aggctgtttt atcaactgga gcaatagata tagttctttc
    22501 agaatttaac tgtactatac cagggcttga accaatagca gataaatata aagtaaaaat
    22561 ggtttgttta gatgatgttg ctaaaaaagc gaatgcagaa tgcataggtt tagacagaag
    22621 taaattagat gaacttagta acactttaat agaaaaagca ttggagtctt ataaggagag
    22681 aagaggaagt attgaaatag atataccaaa agaccatgga tttgaacaat ctttgactgg
    22741 tgtaagtgaa aagaacttga aagcgttttt aggagattca tggaaacctc ttattaattt
    22801 aattgcagaa ggaaaaatta aaggtgttgc agctgttgta ggatgttcaa atatgacagc
    22861 aggaggacat gatgtaaata ctgttgaact tactaaagaa ttgattaaaa aagatataat
    22921 agtactatct gcaggatgtt ctactggagg attagaaaat gttggtctta tgtcacctgg
    22981 agcagaagaa ttggcaggag aaaacttaaa agaagtatgt aagactttag gaataccacc
    23041 agtattaaac tttggaccat gtctagctat aggaagacta gaaatagtag caacagaatt
    23101 agcagctgaa cttggaattg acctaccaca attaccatta gtgctttcag ctccacaatg
    23161 gttggaagaa caagcattag ctgatggagc ttttggtctt gcccttggct tacctcttca
    23221 tttagcttta cctccattta taacaggagg aaaattggta acagaagtat taacagaaaa
    23281 attaaaagac ttaacaggag gacatgttat agtgaatcca gaccctaaat cttcagctaa
    23341 tcaattagaa gaaataataa tagatagaag aaaaaattta gggttaaagg atgtggaatg
    23401 caatgcatag aatttttata aataaagatt tatgtacagg atgtaagagc tgtgtactag
    23461 catgtatgct aaaacacaat aaagattatg acatgtacac tcttgattta gaaaatatag
    23521 ataatgatag tagagggcat atagaattag atagtagaca taataatcca gttccaattc
    23581 tttgtagaca ctgtgatgaa cctgaatgtg tattagcatg tatgagtggt gcaatgcata
    23641 aagatagtga aagtggaatc gtttcatatg atgaagagaa atgtggttca tgttatatgt
    23701 gtgtaatgtc ttgtccatat ggattattaa agccagatga tagaagcaag cagaacatac
    23761 ttaaatgtga tttatgtaaa gatgaggagt atcctagatg tgtagctaat tgcccaagtg
    23821 gagcaattga attacagaag gaggaaaatg atgaattatg tagtgttagg ggctagtgca
    23881 gctggtataa atgcagtaaa aacattaaga gaattagata aagattcaaa tatagtagta
    23941 atttctaaag atgaaaacgt atattcaaga tgtatgttgc atcacgttat atctgaacac
    24001 agaactttaa aacaaataaa ttttgttgat gaagatttta tggagcaaaa taatgtaaaa
    24061 tggattgcag gtaagactgt taaaggtata gacataaata aaaaggtagt tcagacagaa
    24121 gacattactg taaattatga taaactttta atagctacag gtgcatcttc tgctattcct
    24181 cctataaaaa atttaagaga tggaaatttt gtatattctg tgagaaacat agatgatatt
    24241 tataaaataa aagaaaaagc acaaaattct aaaaatgtag ttataatagg tgctggttta
    24301 gttggtatag atgcattagt gggattattt aaatatgagc agttaaatat ttctgtagct
    24361 tttatggaaa agtacatttt agatagacaa ttggatgaat atacagcatc agtgtatgaa
    24421 aataagttca aagaaaaagg tgtaaaattt tacccaagtg catcaataca agaaattgta
    24481 ttggataaca gtaaaaatgt tacaggtgtt gcattttcaa atggtgaagt tttagatgca
    24541 gacatggtaa ttgtagctac aggggttaaa ccaaatgctg actttttaga tggaacagga
    24601 atagaatatg atagaggtat aataatagat gatatgtgcc aaacaacaca aaaagacata
    24661 tatgcagcag gagatgttgt aggtaaaaat gctatttggc ctctggcagt aaagcaagga
    24721 attgtagctg cctataatat ggttggaaaa gataagaaga tagaagatga atttgctttt
    24781 aaaaatagta tgaatttcat ggatatacca actatttcta taggaatgaa tacacctgta
    24841 gatgatagct ataaagtatt aaccagacat ggaattaatg attataaaaa gtttgttttt
    24901 aaagataatg taatctatgg agcagttata caaggagata tttcatatgt aggtgtatta
    24961 acttatttaa taaaaaataa agttgaaata tatgatttag aaaataggat atttgatata
    25021 tgttatgcag acttttttaa tataaaagaa aatggagaat tttgttatag tgtatagtat
    25081 atacgtggag taaggtttat aaatacagta aatcaataaa tagagttatt ctaaaataag
    25141 aaaaatgaca aatcttattt agaaatagct ctattttttg taattaaatt taaattatat
    25201 tcaatttgta aaatattatt ttgaacaatt atacatatac tctctagtta gaggtaaatc
    25261 attttttata cctttagtga aaagtatttg atgtatatct ataataccag tgttgaaagc
    25321 agcagcacaa gaacaaaggt ataaatccca cattcttata aacttatcat caaacatttc
    25381 tttaatcttt ggaagattct cagagaaatt tttttgccag tatagtaagg tttttacata
    25441 atgtaatctt aaactttcta cgtctatagt atgatatttg taatcactac aaatatgtat
    25501 catttctctt aggctaggaa taactcctcc tgggaaaata tattttttta tccaagggtc
    25561 tccataagat tcctgcaatc cacttatata gtgaagtaaa aacaatccac catcttttaa
    25621 aacagcattt acgtttttaa tgaaaagttc atagttttct ctaccaacat gttcaagcat
    25681 cccaacgctt acaactctat caaacataag gtctgatttt tttaattctc tatagtccat
    25741 aagtcttacc tcaagatagt cttgaaggtt ctcttcttta attcttgcgt taaattcctt
    25801 aaattgctct tcgctaagtg taattccaac gccgtgaatt ttatattttt ttgcagcttc
    25861 tattagtaaa aatccccaac cacaaccaat atcgagaagg ctcatacctt cctctaaatt
    25921 tagtttagtt aatatgtggt gaactttatt gacttgagca tcataaagag tatcattatc
    25981 atttctaaag tatgcacaag aataactaag tgtcttatct aaccataagc tataaaaatc
    26041 gttgccaatg tcatagtggg catgtatttc tttcttttga tgtctcattg aatttgatga
    26101 gtgcaataga tgttttacag ctgaattatc aatattgaat ttgtctattt gttttaaaag
    26161 tagatttaaa gttaagaata aatctccctc aacttcaatg tctctattta tgtaggcttc
    26221 tccaagtgct aaagatgtac tttttaatag ttcagacttg ctaatatcat tatttactat
    26281 aactttgaac tgaggggagc cctctccaat tagaaattct tcctcatctt taagcctaac
    26341 tagaaatggt gtttcaccaa atttctctaa atagtctttt aaaaaatttt ttgtaatatt
    26401 catattacca gctccttcta atgtaaatcc agtaagtttc cctgcttaaa atatattata
    26461 gtcctttttt taaaaaagta aaattttttc aataaatttt ttatttaaag gaaatttatt
    26521 ttttgtaaag aaatttcatg ttgtagtata ttttttgctt ataaattttg gtactggata
    26581 taatattaaa ctgttaatga tataataaat ttaatctata aagtaaataa aattttgtaa
    26641 taaatagtta aagctatttt ataaggagat atatgatgga agtatatgct ttggttgggt
    26701 caagtggcac agggaagagc tttaaggctt tagagttcgc atatgaaaat gatatagaat
    26761 atataattga tgatgggatt ttaatataca aaaacaaggt tttagctggg atatcggcaa
    26821 aacaagcaaa tactactata gaagcagtta aaagggctat tttttataat ctggagcata
    26881 gacaagaagt tagagaagca attaaaaaag aaaatataag aaaaattctt attattggga
    26941 catcaaaaaa aatggttaat caaattgtag agagattgtc aattggaagg gtttgcaagt
    27001 ttataaatat acaagatata agtactaaat atgaaataga aatagcaaaa caagctagaa
    27061 aagaaggaaa tcatgtaatt cctgttccag cagttgagat aaaaagtatg gcaagtggtc
    27121 ttagtataaa ttctttgaag agacttttta gaaaaggaaa taataggaat atggtagttt
    27181 tagaaaaaac aattataaga ccaactttta gttatatagg aaagttttat attagtgctg
    27241 atgtaattaa acagattatt gaatatgaag tatacaactt tggaagcatt gatagaataa
    27301 ataaaatcaa tatagagaat tgcaataatt ttatgaatat ttttgtaagt ataaatatca
    27361 atgatttaga aactataaaa aaaattgaag acatacaaca gacaataaaa aagtctattg
    27421 agaaaatgac tttagttaat gtacaaaaaa tagatattaa cattcataaa attaaaaaaa
    27481 taaacttcct ccaatgttaa aaaataaact gagaaaatga tatactaatt tgcaataaag
    27541 tgtgctataa ttaaactgta aatttaggaa atggtttttt ctaaatggag tatacataat
    27601 tataacgtat ttataagggg gctttcaaat gtcaggaaaa gatgtaaatg tcttcgagat
    27661 ggcgcaatct caagtaaaaa atgcatgtga taaattaggt atggaaccag cagtttatga
    27721 attattaaaa gaacctatga gagttataga ggtttcaatt ccagtaaaaa tggatgatgg
    27781 ttctataaaa acttttaaag gatttagatc acaacataat gatgcagtag ggccaacaaa
    27841 aggtggaata agattccatc aaaatgtttc aagagacgaa gtaaaagctt tatctatatg
    27901 gatgactttc aagtgttctg taacaggtat accatatggt ggaggtaaag gtggaataat
    27961 agtagatcca tcaactttat ctcaaggtga attagaaaga ttaagtagag gatacataga
    28021 tggaatatat aaattaatag gtgagaaagt tgacgttcct gcaccagacg taaatactaa
    28081 tggtcaaata atgtcttgga tggttgatga gtacaataaa ttaactggac aaagttctat
    28141 aggtgttata actggtaagc cagttgaatt tggtggttct ttaggaagaa cagctgctac
    28201 tggatttggt gttgctgtta ctgcaagaga agctgctgct aaattaggaa tagatatgaa
    28261 aaaagctaaa atagctgttc aaggtatagg aaacgttggt tcttatacag ttcttaactg
    28321 tgaaaaactt ggtggtactg ttgtagctat ggctgaatgg tgtaaatcag aaggttctta
    28381 tgctatatac aatgaaaatg gtttagatgg tcaagctatg ttagattata tgaaagaaca
    28441 tggtaactta ttaaacttcc caggagctaa gagaatatct ttagaagagt tctgggcttc
    28501 agatgttgat atagtaatac cagctgcatt agaaaactct ataactaaag aagttgctga
    28561 atctataaaa gctaaattag tttgtgaggc tgctaatgga ccaactactc cagaggctga
    28621 tgaagtattt gctgaaagag gaatagttct tactccagat atattaacta atgctggtgg
    28681 agttacagtt tcttacttcg agtgggtaca aaacttatat ggatactact ggtcagaaga
    28741 agaagtagag caaaaagaag aaatagctat ggttaaagca tttgaatcta tttggaaaat
    28801 caaagaagag tacaatgtta caatgagaga agctgcttac atgcattcaa ttaagaaagt
    28861 tgctgaagct atgaaattaa gaggatggta ctaagattta ctcttaataa ataataggat
    28921 ataattattt aagatataaa ggggctgtac atagtacagc ccttaaatta tgtggcttac
    28981 aatgaagtaa gctatttttg ttttaactta taaataactt gataaattat gtaatttaga
    29041 atgtattttt taccaccaca tgattacaaa ttagaggagt tagattgaat ttttagacat
    29101 gaacttatgc attttaaaag caaagacctt aatttaaagt atctagtatt atttttaaaa
    29161 atagtatatt ggtttaatcc atttatatgt ataatggaaa aatatatgga tttggattgt
    29221 gaactttatt gtgatgagag agttttaaaa aattgtactg tacaacaaaa gcaagaatat
    29281 gctctaacta ttgtaaattc aatgaaaaga agtacaaatt tttcaaataa atttgtagct
    29341 ggattaaata aacaatctga tattagaaaa agagtaataa acatgttcag tgaaaaacat
    29401 aaaaatggta tcttaagggc tttgattata agcttgtttt cttctattac attttttaaa
    29461 ttcgattcaa ctagtattat tgaagagaat gatttcttag agaggtcaag atatacgttt
    29521 gaaggaaggc aagaagacat gtttgtaaca atagatttta tatatgcaga tgtacctata
    29581 aaacttagaa aagagcatga agaaaactgt agagtcaatg gaatagtacc aaaagattca
    29641 gattcaatag aaattattcc tagattttat aaagattgta tggatttaaa aaaataagtt
    29701 agacttaata ttgatgttga tattatgaat tttttatatt taagcaattg aaaattttat
    29761 tatagattca attttatatg tgtttaagta tgaaatttat agatagttga aataattaaa
    29821 tatgattgat gcataggaat cattactaag ctataattaa ttatagggga gtttaaataa
    29881 attatgagtt acacaatcat aaaatgataa aaatagttat ctgttacaaa aaatgtatct
    29941 ttttaattaa taaagataat atgaaaactt aaaaggcaaa taaacttaaa tataaaatac
    30001 tcttaaataa gataggagaa aaatgtatgt ctaactttat tctaaatggg atttttagaa
    30061 cagttatagt aagtagtcta agcattcttc tgatattgat tttcaagaaa aatgtattta
    30121 aaagatttag caaaaaatt
//
LOCUS       DAEPIN010000052        29933 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.052, whole genome shotgun sequence.
ACCESSION   DAEPIN010000052 DAEPIN010000000
VERSION     DAEPIN010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 29933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29933
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.052"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            complement(757..1266)
                     /locus_tag="KR250_002514"
     CDS             complement(757..1266)
                     /locus_tag="KR250_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HBH0068443.1"
                     /translation="MVMFLFKIDDYIMYGMTGVCKVMDITNERFTNGIKKEYYVLSPI
                     YSNNTVIKIPVDNEKVPMRKLLSKINVLSLINDIPNMDTSWIDNEKLRSEQFKKILRG
                     GKCEELIKLVRSIDNNREYVKSIGKKTHQADDNIMKEAERLLNEEFATILDISPNEVS
                     SYISSHIPQ"
     gene            complement(1477..2886)
                     /locus_tag="KR250_002515"
     CDS             complement(1477..2886)
                     /locus_tag="KR250_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0068444.1"
                     /translation="MENYFDKQAKELYNKASEIINTHSMLKANRANEKFDIDIPQDFK
                     YEFFSLVDKVNLSLMEEEDNFYGYFLFQMSREIRFDISSPTGVNFKGAKYVIYFNPII
                     FLTLDIKQMETTIKHEIHHILSMHLMRAKELKGKYSTLAINMAMDIVVNKFLNNLPPY
                     ATTLEWVNLKYSLKLEPYEPFEYYVEKIQTELDLMEVDEDGEEDDSDKYGDIEIDYSP
                     ERTHDIWDESNEIDENTLREFTEKFINSSQKGKVPAYLESMISSLKNSKGELPWNLYL
                     NRIMGTVENNKKKTITRRNRRQPNRLDLRGELRSHKSEIAVALDVSGSISDEEFKQAI
                     KEVLSIVKNYNHEITIIECDNEIRRVYKVRTEKDIKDRIRIGGGTRFSPVFEYANNKK
                     INLLVYFTDGKGENKLQVIPRGYKILWVISGRGDKISLKEPYGAVKKLTKVKTNDEIL
                     EINDVRNDGYSMNSQAPIL"
     gene            complement(2890..4020)
                     /locus_tag="KR250_002516"
     CDS             complement(2890..4020)
                     /locus_tag="KR250_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBH0068445.1"
                     /translation="MNFIDTLKSVDLILSTGEVPLIVGESGIGKTALANKLAKENDWS
                     LIVIDGNLLKEGEIGGLPTIESYVGVNSNGYKTEKKTTVYAVHNKLREIDEEISKSKT
                     VLLFIDEINRCEHTVQQELMNLILNREINGYKLHDDVKILAAMNPSSKYGSDFDYQVV
                     DMDAAQENRFVWLNMESDHTQWIKWAIDEGIERKVIEFISTFPEYLHKINEDDVRATP
                     RSYERVSKIYKVYKEKNNSIPRAVFLNVVKGNVGKVIAEEFISFIESNSEPLISYEDV
                     FLGESIDESIVERVKNESHTRLYLSAMNILKDLELNIKNDKYESNHYINRFIEFLKMY
                     PVDLMIGIMKDIRNSYIEVYKKAIENEEFVKSYFESYSLIRG"
     gene            complement(4032..4835)
                     /locus_tag="KR250_002517"
     CDS             complement(4032..4835)
                     /locus_tag="KR250_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0068446.1"
                     /translation="MEKLRVIEMIKNNPNIIATIDNPTDEMKLLAIKENGLVLEYINN
                     PTREMQELAIDNNIRAIKFIDNPTEDMMIKAVNEGWSILDYIKNPTDKVIEMAINQAG
                     WAIKYAKNPSEELQLLAVRKNYDSIKFIKQPYESVQEEAVRISYDALRYIDSPSYNAE
                     LIAIKNNEAAISFITDLDKNKMLEFLKVNILVIKYILKKVSKDELESVLKEVLSKEDV
                     EEKYIRDFLNCSVIDRNSESIPIDKIMFIYKYGSKTAKKIAVDEKLKIM"
     gene            complement(5217..6098)
                     /locus_tag="KR250_002518"
     CDS             complement(5217..6098)
                     /locus_tag="KR250_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline iminopeptidase-family hydrolase"
                     /protein_id="HBH0068447.1"
                     /translation="MKITEGYMPFKGFKTYYRIVGENTEGKKPLVLLHGGPGSTHNYF
                     EVLDKIAESGRQVIMYDQIGCGNSFVEGHPELFNADTWIEELIELRKHLGLDEIHLLG
                     QSWGGMQAIWYAIEYKPKGIKSYILSSTLSSAKLWEKEQKRRISYMSEVDQKALLDAV
                     NTGDYSSKEYNDALERFMEMYCAGEVTEDSPECLRRPKKSGSEAYIVGWGQNEFSPTG
                     TLSGYEFTDRLHEIKEPCLVTSGAIDLCSPYIAKTMYDRIPNSKWELFEYSRHMPFVE
                     ENEKYIKVLTEWLNAND"
     gene            6554..7528
                     /locus_tag="KR250_002519"
     CDS             6554..7528
                     /locus_tag="KR250_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0068448.1"
                     /translation="MNNINSLKVIKLSLGCSLAIFIAWLLKLEYSMVAGVIVLLTVKD
                     TKRETLKGSIGKIYGFLLCTIFSYLCFNILGYNLTSFSIFIFIIIPLCFLLNIQDVIA
                     MCVVIASHYYLQGETSIKWILNEAGIFAVGTGIGVLINMYIPTNIHKIHEVQKKLQEE
                     VSIVLIDIADIIVNPKKENGYSRDLYTLNSLIDSSISETYDNINNTLLSDTRFFLEHM
                     DIIKSQRDILENLYSYVSQLNSTPPQAHILSAFIHKIGYTEFEAETGNLLLEELKRLM
                     LSMKNQPLPVDRTEFENRAILFLCLTELKQFLVNRKHAQILRDNNFYK"
     gene            complement(7644..8705)
                     /locus_tag="KR250_002520"
     CDS             complement(7644..8705)
                     /locus_tag="KR250_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HBH0068449.1"
                     /translation="MKKIRKLTTKLLITTIVLISGMSMTVYGMTAKEVTAKTPKSYVT
                     GTNSVYGPKLSQAQLNSVAQATADFMNKKITKNMTTDAKILVAYNHIKNNTTYVDWNA
                     VEGANTAYTLVTKKGACSGMARSMKALCDAMGIESYYVHSTSNDHQWNLIRFGDGRLY
                     HVDIDANKSAGKDIIYKSLSHPLPFDKTAYPKVGAIVQTVKPEQTQKPAPPGKHTMLP
                     HQEGAKAVGNFGKKGEDINEKHVMYEGVRCSVVNSSVNTITFTGSESNLDPSNLTIAV
                     IGFMNTPYGMFALAPEGKPVEYPFKYDKPFSLDFSGKNRSGAELAKYCKKNDVTIKIE
                     ITDEKANIGLGQIMYVRYE"
     gene            complement(9317..10168)
                     /locus_tag="KR250_002521"
     CDS             complement(9317..10168)
                     /locus_tag="KR250_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBH0068450.1"
                     /translation="MSCILKLKQIYEDLTEVDKKIADYILNNTEAISKLSVSELASNS
                     KTSTASIVRFSRKMGYSGFGDLKIEIAKDLMGKENEYTYVDENYDCNIDSVINKITNK
                     NIETINQTRLLNEKDIIKEAVEQIIKAKNVYIFGVGGSALVALDLQMKLLRINKQAFT
                     SLDSHTQLMVSSNVDKEDIAIAISYSGESKEVIKSIENAKLKGCKVICITKYSDNHLS
                     KISDLKLVVPNIEKRLREGAISSRIAMLTLIDIIYISIIQENLNEAEQKLQETKRILD
                     YLNINNA"
     gene            complement(10185..11105)
                     /locus_tag="KR250_002522"
     CDS             complement(10185..11105)
                     /locus_tag="KR250_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA reductase"
                     /protein_id="HBH0068451.1"
                     /translation="MYFLGVDGGGTKTTFTLVDEELNIVGTITKGTCHYNQIGFDNLT
                     KLLITGLEEVCKDAKINVEEITYAFVGLAGYGKIKEVLYALEVATKNAYSHINYTLGN
                     DVEIALAGSLNGEKGINIIAGTGSIAQALDKDGNLHRCGGWGYVLGDEGSAYYIGMAT
                     LKMFTMQSDGRCSKTKLYDLIKRHLNIENDYDIIKYVNDEIQGDRIEIAKFATICLKA
                     VIEGDNTASKIFDDAAYELSRLIIGLEHHFEQGTKIKVSYSGGVFKSGDLILEPLKKY
                     LNKTRFGIVRPVLTPDLGACLLAKKKYYSK"
     gene            complement(11215..12129)
                     /gene="murQ"
                     /locus_tag="KR250_002523"
     CDS             complement(11215..12129)
                     /gene="murQ"
                     /locus_tag="KR250_002523"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HBH0068452.1"
                     /translation="MLIKTLENLVTEGRNKNTLQIDKEDTLGIIELINNEDKTVAYAV
                     EEQKESIAKAVNIIVDRMKQGGRLFYIGAGTSGRIGILDATECPPTYGVDFELVQAII
                     AGGNQAIFKAIEGAEDDKELGKQDIIDRGVTSKDVICGIAASGRTPYVIGAMEYAKEL
                     GCAVLSITMNPNSEMSKKADLPINIIVGAEVIMGSTRMKSGTAQKMVCNMLTTASMVK
                     MGKVYSNLMVDVKTSNEKLVERAKRIIMIATNVKYDVAEKFLEEADNSVKLAIFMIKS
                     GLDKDSAKSILDRQEGYISEALKSIEKL"
     gene            complement(12228..13325)
                     /locus_tag="KR250_002524"
     CDS             complement(12228..13325)
                     /locus_tag="KR250_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HBH0068453.1"
                     /translation="MGRLGISIYPEKTTSKEIYDYIDKAAENGFTRIFSCLLSVKDTK
                     ENIIKKFKRINEYAKFKGFEVILDVNPRVFGELEISYKDLTFFKKTKADGIRLDIGFT
                     GSEEALMTFNPQNLKIEINMSNNTNYIDTIMDYQPNKENLTGCHNFYPHRYSGLNLEH
                     FIKCTERFNKYGLRTAVFVTSQNETTFGPWPVTDGLPTIEMHRNLPIDIQVKHIIAMN
                     NINDIIISNCYPTYEELKVLGSMRKDMVTFDVAIEENIREVEKKILFEEIHFNRGDIS
                     DSLIRSTQSRVKYKGYKFELFNTPKIIKRGDIVIESSEYGHYAGELQIALTDMKNSGK
                     SNVVGYIRKEELFILDYIKPWQKFNFKPSTK"
     gene            complement(13558..14904)
                     /locus_tag="KR250_002525"
     CDS             complement(13558..14904)
                     /locus_tag="KR250_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBH0068454.1"
                     /translation="MLDKFTEFLDTKLSTSMARLAEQRHLRAVRDGIVASLPLIIVGS
                     FFLILAFPPLPDSWGVTQYLKENAVTILLPYRMTFFIISVYSTFGIGYSLAKSYKLDG
                     LTGGLLATATFLLTIVPTAIEGLTPELLKIVEKSPELLAWYNSIPQGWQMPMANLGGG
                     SMFVAIVTSIIGVEIYRFTDKSKFKITMPEAVPASVTRSFEALTPAALIIILMAIVTY
                     FLGFDWHGFIAKLVTPLVSATDTLPSVIILIFLITFFWSFGIHGVSIIGSLARPLWIQ
                     LLDANTASYAAGQAVPGIAAEPFFQWFIWIGGSGATIGLALLLAFRAKSAYGKSLGKA
                     TVIPAIFNINEPIIFGAPIVLNPILIIPFVVAPIVMATVSWFAMSANLVSRVVVTAPW
                     TLPGPVGAYLATGGDWRASVLNLLCIAISIIIYYPFVKMYDKRLLHEEKVEEQEAY"
     gene            complement(14993..15301)
                     /locus_tag="KR250_002526"
     CDS             complement(14993..15301)
                     /locus_tag="KR250_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBH0068455.1"
                     /translation="MRVLFVCSSGMSSAIAVNALQKEGAKNGIDIDVLAVGTQEFENE
                     VKNGWDIAMVAPQVRHRFDYLKAFADEVSVPCALIQAQAYSPLGGPKLLKQVQELLSK
                     "
     gene            complement(15563..15961)
                     /locus_tag="KR250_002527"
     CDS             complement(15563..15961)
                     /locus_tag="KR250_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0068456.1"
                     /translation="MSNNKKKKRKKNNNYKNAKSTKSNKNTKKQMEKNQRTDYNIPTI
                     SFKSGLILIVSSLVSTVVFPLLLSIFGIDNRFGVVIGNSLITSFAVAYTRYFVETKRG
                     FSLGFFRLYILFALSFAIIGYFWIYKGIHI"
     gene            complement(15963..17123)
                     /locus_tag="KR250_002528"
     CDS             complement(15963..17123)
                     /locus_tag="KR250_002528"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HBH0068457.1"
                     /translation="MITMYSVGLMSGTSLDGVDAVLAEISGNGRNTKVKQIEFITLEI
                     PKDIKDEIRKCCIEEESSVDLICSLNFKLGYLFSKAVKSVCHKANFHIANLDFIASHG
                     QTIFHIPRSYNNFVSSTLQIGEPAVIAYETNTKVISNFRVMDIAAGGEGAPLVPYSEF
                     LLYSDNNKNLALQNIGGIGNITIIPKSCNIDDVFAFDTGPGNMIIDGVCQRLFNRKYD
                     KNGYFASKGKINEEMLKDLMSHKYLSQAPPKSTGREVFGQVYLDNMLNKYKHVDKYDL
                     IATVTMFTAKAIYYNYKNFILPKVNVDTLLIGGGGAHNLTLIGYIKELLLEVEVLTQD
                     EYGYSSDAKEALAFVILGNETLNNSFSNVISATGAKNKVILGNITPKPFGGK"
     gene            complement(17554..18573)
                     /locus_tag="KR250_002529"
     CDS             complement(17554..18573)
                     /locus_tag="KR250_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBH0068458.1"
                     /translation="MLVKYLKGRSKYIALPLVVVSIINIYLFAIDIFKNKYSELIYLD
                     FLVLFIIMVFFVIDYINFRNSYTNLYNCIENSGEIDSYLVDGQSFEENLIKDIIENLK
                     IKNNSDIETYKQSLKELDEYIAKWVHEIKIPISSLSIITDRLSSIEDSLDIKNQVAKI
                     NFLVNSILYSSRSNTMFEDVFINKFNLEKLVKMSIKNNSFLLIKNNVEVSLNELENDV
                     YTDSKCMSYVLDQIINNAIKYSKEVGKIEFNSKKLENGVVLSIKDFGIGINEEDISRV
                     FDKGFTGKNGRNQLYKSTGMGMYFVKKMIDSLGHEIEVCSKIGSYTIFNIYFYDISDY
                     LSIDS"
     gene            complement(18567..19247)
                     /locus_tag="KR250_002530"
     CDS             complement(18567..19247)
                     /locus_tag="KR250_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0068459.1"
                     /translation="MYRILLVEDDIDLSKEIALALEKWGFKVELIDDFEVVLDEFIDK
                     KPDVVLLDVNLPLYNGFYWCEKIRAISNVPLIFLSSRDSDMDLIMGINNGADDYITKP
                     FSIEILVTKINGIIRRVYNYSDSNSILYCEDLMFDVGKGIIKHKYKDKSIELTKNEIK
                     ILTLLLKNKNRVVSRESLMMTLWDNDEFVTDNALTVNMNRLRSKVKELGFDDFIKTKK
                     GIGYIIQC"
     gene            complement(19399..21306)
                     /locus_tag="KR250_002531"
     CDS             complement(19399..21306)
                     /locus_tag="KR250_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBH0068460.1"
                     /translation="MSILGIAYNNFKSNIRTYLAFFISMVFSVVVLTNFELLRYGDAI
                     NVLQEENKKFTLSVLVAVIMILSVFLFFFIWYATNIFFKNRSKEIGILSFMGLDLYTI
                     GKIYFVENMLIGISSCITGIIIGIITSRFFQVVIIKLSGFNIKVSNGLSIEAILYATL
                     IFISIFIIMAVKGFITICRSSVINLINISKKQEKIPKVGIGIYILAIVSIIVIGYGYY
                     LSTNIRTGDITSVLPVIGIIIVGTYGLFKSVMPVIFDIIIKNKKILFNGNNIIAINNI
                     NYRLNKNYKTYAIIAIIITTTISTLGASVAMKHIHKNAQEQRNVYTISIVSMDKDEVN
                     KKQIENIIKDTNKIKYSVNPELTILKKTDIKEAYDTTDTIMKYSDFCNTLKVNGNDKE
                     LKEYGERLVSGNNVIELKSPQTIASIATKEAKIKLDGQLYNISRGEVKVPVLGTGLNQ
                     SIIVVNDDTYFKLRDKGQQVYFYGAKVFQEENSQVMFNNIEKNLSLKNGYIQNGYKAQ
                     GESQWMKFAYVVLVFLFLVFAIVAGSIIYMKIYSDAYEDKEKYKILLKIGTTEKDINR
                     AILKEVAIFYTLPMLSATISSYFALRLAGDLLMTDLFGIYILSLVICLIIFIIYGAVS
                     VNKFKSVVYKN"
     gene            complement(21307..22059)
                     /locus_tag="KR250_002532"
     CDS             complement(21307..22059)
                     /locus_tag="KR250_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0068461.1"
                     /translation="MKEILKIKNISKDYGIKGFKTNVLKNISLTVNEGDFISIMGPSG
                     AGKTTLLNLMSTLDKQTSGEIILDGINISKVKNNELSKLRREKIGFIFQDYNLLDNMK
                     LMDNIALPLALGKKKSKEIEAKVFSIAKKFGLENHLDKYPYQLSGGQKQRGAAARSLI
                     TNPTVIFADEPTGALDSKSAYELLESLEKINRENNATIIMITHDPLTASYSNEVYMIN
                     DGNIKCKLNKGNSRKEFYGKIMDMLASMGGEM"
     gene            complement(22375..23529)
                     /locus_tag="KR250_002533"
     CDS             complement(22375..23529)
                     /locus_tag="KR250_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBH0068462.1"
                     /translation="MINKAIKFKFIIFYFFSYIIFAFASTKFTPFLSKLGYSAFERGI
                     ILSSYAITNILFQLLFGVLADKYQTMKKIVLISLSVYGIASVIMFSTESATFIIYLLL
                     VALSGGLLNTCCSLYDTWIIGCGDNINNYLSFIKTFGSIGWAIGSMVASYLILRFSYF
                     GLSVAIGLIVVLLIINILLLPDIEKINKKINVTNKDIIELLKDKKYILLVCILFLLYS
                     MVVANNCTVIDKMIALNATNSQISLKWSLQSLLEIPTYLAGSYLLKKYSSLNLLKLSA
                     IMITVQFLLFALTNDSNIIIILCVFQVFSTPLILITSKRLIFEVSPKKLRSSSQLIAL
                     SIFMGGSSLIIPTVAGFLSINIGYNYTLMVLSLFGCLAYCLIVLLNYLQE"
     gene            complement(23532..23999)
                     /locus_tag="KR250_002534"
     CDS             complement(23532..23999)
                     /locus_tag="KR250_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBH0068463.1"
                     /translation="MFFKRKKEAITINSYIDGKIINLGEIPDKVFSTKVMGDGFAMIA
                     KDNILKSPTKGVISFIAQTNHAIGIKLQNDVQILIHIGLDSATTKKENFKLEVKQGQE
                     VNMGEKLVEIDNKLLENTLYIPLVIVENPNTVKYHLSNLNQEGNSGIKVINFD"
     gene            complement(24013..24501)
                     /locus_tag="KR250_002535"
     CDS             complement(24013..24501)
                     /locus_tag="KR250_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0068464.1"
                     /translation="MITNKIKNITAVLKDENIFLSVVMVCFLILPVLYIVYLELTSLF
                     TNISFSQLLNNDVNASLNLISNFSAVYSAYVIYNIKKNKEIKENMIAFYLVLLGQIFQ
                     IYTITIVIMFTYIYKFVGIKQIISSIKELQWGKSYKALFSSIAVCLISLFVMIIRIKL
                     IM"
     gene            complement(24515..25846)
                     /locus_tag="KR250_002536"
     CDS             complement(24515..25846)
                     /locus_tag="KR250_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HBH0068465.1"
                     /translation="MERKRQRILIAGGGSTYTAGIVTMLIESVAKFPIESIKLYDNND
                     ERQRKVAEACAIIVREKNPEIKFSYTTNPEEAFTDIDFVMAQIRVGLYALREQDEKIP
                     LKYGVVGQETCGAGGIAYGLRTIGPIIEMIDYMEKYSPNAWMLNYSNPAAIVAEACRV
                     LRPNSRIINICDMPVCLEEIFARVLGLNSRKDFDVRYYGLNHFGWWTSIKDKEGNDLM
                     PKLQEYCAKKGYEEFTPQGQHKESSWLETMRAAKDLLEIEPTTLPNTYLKYYLMADET
                     VEHANPNYTRANEIMDRREKDTFEECERIIKNGTARDTWFDASEHSQFIVELACALAF
                     NTQERFLLIVPNNGAIENFADDAMVEIPCLVGKDMVEPMSIGKIPTFQKGLMEQQVAS
                     EKLAVEAWIEKSYQKLWQSFTMSKTVPSAKVAKEILDEMIVANKDFWPTFK"
     gene            complement(25860..27425)
                     /locus_tag="KR250_002537"
     CDS             complement(25860..27425)
                     /locus_tag="KR250_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003233606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBH0068466.1"
                     /translation="MMKKIQRFGGAMFTPTLLFAFAGIMVGFSIVFQNQSIMGSLATP
                     ENIWYQFWGVISSGAWMVFNQLPLLFAISLPIALAKKQQARACMEALATYLTFNYFVG
                     SMLSFWGKSFGVDFAAEISAGSGLVSIAGIKTLDTGMVGALLISGIVIYIHNRFYDKE
                     LPDFIGLFRGSSLVVAICFFVMIPVALLTCFIWPHIQNVIRHLQTFFINSGNIGVWCY
                     AFLQKILIPTGLHHFVYAPICYDSVVVPGGTSVYWATHIQDFQTSAKTLKEMYPIGFS
                     LSGLSKVFGSLGVFGAFYVTAKPEKKKKVLGLMIPATLTAVLTGITEPLEFTFLFVAP
                     LLFLVHAFLDACLQTISFALGVVGDFGGGIINWVVLNWLPLGMYHWKVYVVQVVVGII
                     FSFIWFFVFTFLIKKFDMKTPGREEDSEETKLYTKNEYLETKDEKGNKLSKASQQASE
                     YIKLVGGAENVVDVTNCATRLRLTLKDDSIISKEEDFKAVGAHGLVHNGKAIQIIIGL
                     SVPSVREEFENLL"
     gene            complement(27595..28380)
                     /locus_tag="KR250_002538"
     CDS             complement(27595..28380)
                     /locus_tag="KR250_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBH0068467.1"
                     /translation="MRLEEMIFLNYNKLNDTDLLIWKYITANKKHCSKMSINELAMKC
                     NVSRATITRFVKKLDLKGFSEFKVLLSWESEKVHVIENQSFDIACNSIIKYVEDQKNK
                     DYDALCKLLYESRTIYAYGSGDIQNVVAKQIKRMFLSCQEIIYDFEGRTFDDSFYNIV
                     SKNDVMIIISLSGENENTLNIARRLKLIGTKIISITEFKNNTLTDLSDESIYISATNI
                     SFLQMHPSYKLTMLYFILIELLFIKYSIYKRHRMITEGIDCKL"
     gene            complement(28547..29746)
                     /locus_tag="KR250_002539"
     CDS             complement(28547..29746)
                     /locus_tag="KR250_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S41"
                     /protein_id="HBH0068468.1"
                     /translation="MKKKVLFLVIILAFIILLGNSTYSQKNQLSEEEKVDDFNYVYNV
                     VKTGYPYLGVNKRLNNIDWLANKDEYTKRIKNTENDEEFIEEMSSILSDLNNKHTEVI
                     DNKKRYELFKKSYSNNDWYDFLNDKKVVDRYDSMNLKIKMPDDIFVKKELILKDVIKG
                     QIGYMYLPSMSSKNGSINNDLKIIGDYIDTLEKHKALIIDIRGNLGGSDSYWQGIVSK
                     LIKNDVKINGYRIYRNNNEIVKQYTNARNIKLNPVENLPTSVKENAPKEIIKDFNDFE
                     DTSYTIKSTDDSKFKGNIYLLVDRKIYSSSESFAMFCKETKFATLVGETTGGDGGCID
                     PVLFNLKNSGLIVRMASCMYLNKNGICDEEFKTIPEFKVKDCKRTADFKDDNCIRKAL
                     ELENINY"
BASE COUNT         9931 a         5171 c         3011 g        11820 t
ORIGIN      
        1 aaatataagt gaatgctatc attttaagta tcttataagc aatgtaacta tatatctcta
       61 ataactactt ttttaacatg cttaaactat ttataagcta ttatatttat gtattaacct
      121 aataactttt tcactaaaaa ataacaatct attcaaagat aaactatagt acttcttcta
      181 ctttgtttca ataaaatact aaataaattt cgtacatcca caatttttca ggctacacta
      241 taaatataca atctttatct tagttataag tttaagaata tttttagtct tattttgtcg
      301 aatatctttt taataaaact tcataattct aagcatattt ttacactgtg ttttcatgta
      361 caaaaataat gcttacaaat aaacaacata aataaaactt aaataagatg gattctattc
      421 aacatagtat ccatcttatt cattcaaaac ctaaaaggat tttttataat attatatatt
      481 ttaaaatcta agtaactcat tatagtatca atttcactta aagcttacaa tacacttatt
      541 aatcttgttt tatattaaaa acatctacaa taacaattta aatctttaag tgatatccat
      601 ctcacaccta aaggagtagg ctctccgcac tattctgtaa ttcttcaata caatcatcat
      661 tattttttat ataatttaat tatatatttt attttaccaa aaccagcata cataaataat
      721 acttaaaata gattaaataa aggatactaa aacactttat tgaggtatat gacttgaaat
      781 atatgaacta acttcattag gagaaatatc taaaatagtt gcaaattctt catttaataa
      841 cctttcagct tctttcatta tattatcatc tgcttgatgt gtttttttac caatagattt
      901 tacatactct ctgttattat ctatacttct tactaatttt attaactctt cacatttacc
      961 acctctgagt atttttttaa attgctcact tctcaatttt tcattatcta tccatgatgt
     1021 atccatatta ggaatatcat ttattaatga aagcacatta attttagaaa gtaactttct
     1081 cattggtact ttttcattat ctacaggaat ttttattact gtattattag aataaatagg
     1141 actcaataca taatactctt tttttattcc atttgtaaat cgttcatttg ttatatccat
     1201 gactttgcag actcctgtca taccatacat tatataatca tcaatcttaa acaaaaacat
     1261 caccatcctt aataaaaaaa atagttttga caactaaatc caccttgcca aaactatctg
     1321 ctatatatta tattttaaca cattttaaga cttaaatgta agtaaaactt atattattaa
     1381 aattaaaaaa tacattaacc attgattaat gtatttttta gatttttata acaagcatat
     1441 caaaataaat taatacagct tatctcctgt gaatacctat aaaattggtg cttggctatt
     1501 cattgagtac ccatcatttc ttacatcatt tatttctaat atttcatcat ttgttttaac
     1561 cttagtaagt tttttaactg ctccataagg ttcttttaat gaaattttat ctcctcttcc
     1621 agaaataacc cataaaattt tataccctct aggtattact tgtagtttat tttcaccttt
     1681 accatcagta aagtagacta acaaattaat ctttttatta ttagcatact caaagactgg
     1741 agaaaatcta gtacctccac ctatcctaat tctatctttt atatcttttt cagttctcac
     1801 tttatataca cgtctaattt cattatcaca ttctataatt gtaatttcat gattatagtt
     1861 tttcactata ctaagaactt ctttaatagc ttgtttaaac tcttcatcac taatacttcc
     1921 acttacatca agtgcaactg ctatttctga cttatgactt ctaagttctc ccctcaaatc
     1981 taaacgattt ggttgtcttc tatttcttct tgttatagtc ttctttttat tattttcaac
     2041 tgttcccatt attctattaa gatataaatt ccatggcaat tcacccttac tatttttaag
     2101 tgatgatatc atactttcta aataagcagg aactttacct ttttgagaac tgttaataaa
     2161 tttttcagta aattctctaa gggtattttc atctatctca ttggattcat cccatatatc
     2221 atgagttctt tcggggctat aatcaatttc tatatctcca tatttatcac tatcatcttc
     2281 ctcaccatct tcatccactt ccatcaaatc taattcagtc tgaatctttt ctacataata
     2341 ttcaaaaggt tcatatggtt caagttttaa ggaatacttt aaatttaccc actctaaggt
     2401 agttgcatat ggtggtaaat tgtttagaaa tttatttact actatatcca ttgccatatt
     2461 aattgctaat gtactatact ttccttttaa ctcctttgct ctcatcaaat gcatagataa
     2521 tatatgatga atttcatgtt taattgtagt ctccatttgc tttatgtcaa gcgttaaaaa
     2581 gattattgga ttaaaatata taacatattt agctccctta aaattcacac ctgtaggact
     2641 acttatatca aatcttattt ctcttgacat ctgaaacaaa aaatatccat aaaaattatc
     2701 ttcttcttcc ataagactta aattcacttt atctacaaga ctaaaaaact catacttaaa
     2761 atcttgtggt atatctatat caaatttctc attagcacga tttgccttta gcatagaatg
     2821 agtatttata atttcacttg ctttattata aagctctttt gcttgtttat caaaataatt
     2881 ttccatacat tatcccctta ttaaactata agattcaaaa tatgatttta caaactcttc
     2941 attttctata gcttttttat atacttcaat ataactgttt ctaatatcct tcataatacc
     3001 tatcattaaa tctacaggat acatttttaa aaattcaata aatctattaa tatagtggtt
     3061 tgattcatat ttatcattct taatatttaa ttctaagtct tttagaatgt tcatagctga
     3121 tagataaagt cttgtatgac tctcattttt tactctttct acaatagact catcaataga
     3181 ttctcctaaa aaaacatctt cataggatat taatggttca gagtttgatt caataaaact
     3241 tataaattct tctgcaataa ctttacctac attacccttt acaacattta aaaatactgc
     3301 tctaggaata gaattatttt tttctttata aactttataa attttagata ctctctcata
     3361 acttctagga gtggctctta catcatcttc atttatttta tgcagatatt ctggaaaagt
     3421 ggaaataaac tcgataactt ttctttcaat accctcatct attgcccatt ttatccattg
     3481 tgtatggtca ctctccatat ttaaccaaac aaacctattt tcttgggctg catccatatc
     3541 taccacttga taatcaaaat ctgaaccata tttacttgat ggattcattg ctgctaaaat
     3601 ctttacatca tcatgcagtt tatatccatt aatttctcta tttaaaatta aattcataag
     3661 ttcttgctgt actgtatgct cacaacgatt tatctcatct ataaataaaa gaactgtctt
     3721 acttttagat atctcttcat caatttctct taatttgtta tgaacagcat acactgttgt
     3781 tttcttttca gtcttatatc cattagaatt aactcctaca taagattcta tagttggaag
     3841 accacctatt tcaccctctt taagtaagtt tccatcaata acaattaaac tccaatcatt
     3901 ttctttggca agcttatttg ctaaagctgt ttttcctatt ccactttcac ctactattaa
     3961 aggtacttct ccagtggata atattaagtc aacgcttttt aatgtgtcta taaaattcat
     4021 ttttctctct cctacataat ctttaatttc tcatctacag ctattttttt tgctgtctta
     4081 cttccatatt tataaataaa cataatttta tctattggta tactttcgct atttctatct
     4141 ataacactac aattcaaaaa atctctaata tacttttcct caacatcttc tttggataat
     4201 acttctttta atacactctc taattcatct ttagatactt ttttcagaat gtacttaatc
     4261 actaaaatat tcacttttaa aaattccaac attttatttt tatctaaatc agttataaag
     4321 ctaatagctg cttcattatt ttttatagct ataagttctg cattataact aggtgaatct
     4381 atatatctca aggcatcata acttattctc actgcctcct cttgtacact ttcataaggt
     4441 tgttttataa actttatcga atcataattt tttcttactg ctaacagttg taattcttca
     4501 cttggatttt tagcatattt aattgcccat cctgcttgat ttatagccat ttcaataact
     4561 ttatcagttg ggtttttgat ataatccagt atactccatc cctcatttac tgcttttatc
     4621 atcatatctt ctgttggatt atctataaat ttaattgctc taatattatt atctattgct
     4681 agttcttgca tctctctggt tggattattt atatattcta gtacaagccc attttcttta
     4741 atagctaaca atttcatttc atcagttgga ttatctattg tagctataat attgggatta
     4801 ttttttatca tttcaataac tctcaatttt tccataatgt gcctttcttt ctataattat
     4861 ttttatatct atattaatta taataatcta ttcctattta tataattgtt tcttattata
     4921 attaatatac tttttaatca aatttaaaca gaataattat accaatatat aaaaaaattt
     4981 ctaaaaaaca ataaaattaa gttataaaca ccaatatatt tctattattt caatacgtat
     5041 acaattagat tagaatctaa cgctagtaat aaaaaattac tttcccaaag tagatataag
     5101 cctcatttta tcgttcaaat aatgaaaata tatttgattt catgcaaaaa gagtgcctaa
     5161 tgaactaaaa agttcatttt gagacactca tttttttata taagtttata tagtttttaa
     5221 tcgttcgcat tcaaccactc agttaagact tttatatatt tctcattttc ttcaacgaat
     5281 ggcatatgtc ttgaatactc aaataattcc cacttactat taggtattct atcatacata
     5341 gtctttgcaa tatatggtga acataaatct attgctccac tagttactag acaaggctct
     5401 tttatttcat gtaatctatc tgtaaattca tatccagaaa gagttcctgt aggagaaaat
     5461 tcattttgtc cccatcctac aatatatgct tcagaacctg attttttagg tcttctcagg
     5521 cactctggag aatcttctgt cacttcacct gcacaataca tttccataaa tctttctaat
     5581 gcatcattat attctttgct agagtaatct ccagtattta ctgcatcaag taatgctttt
     5641 tggtcaacct cactcatata tgatattctt cttttttgtt ctttctccca aagttttgca
     5701 gaagaaagag tagatgaaag gatgtaagac ttaatacctt ttggtttata ttcaatagca
     5761 taccaaatag cctgcatacc tccccaagat tgacctaaca gatgaatttc atcaagtcct
     5821 agatgttttc ttagttcaat aagctcctct atccaagtat ctgcattaaa tagctcagga
     5881 tgcccttcta caaatgaatt tccacaacca atttggtcat acattattac ttgtctacca
     5941 ctttcagcta tcttatccaa tacctcaaaa tagttgtgtg tagaacctgg acctccatga
     6001 agtaatacta atggcttctt cccttccgta ttttctccta ctatacggta atacgtttta
     6061 aaacctttaa aaggcatata tccttcagta attttcatgt ttatctcctc caatttgtaa
     6121 ttataaacaa taatcaagtt cattaacaat actatttaaa acctcttata catatttgat
     6181 tatgtatttt aatctttata cctactttta taaatagtat aacataaaag ttaaaaatct
     6241 ctatactaaa ccaattataa aattatatta actatataat tttatttttt aataatagta
     6301 tatacatatt tgattatttt catttgctat ttaaaatcaa ttttaagttt tcttcttata
     6361 ataaacttcc tctttgtggt ctcatgttag tctctaaatt taaaatttta caaaaaattt
     6421 tcataaaata aaattatatt gattgtatct aaacaaatag ggcaataaaa taattaagta
     6481 tagttactta cctttcaagc aatattagtt ttatgttaga attttattat attgatttac
     6541 aggaggtttg catttgaata atattaattc acttaaagta ataaaattat ctcttggatg
     6601 ttctcttgct atatttattg cttggctatt aaaattagaa tattcaatgg ttgctggagt
     6661 tatagtacta cttacagtaa aggacactaa aagagaaact cttaaaggct ccattggaaa
     6721 gatttatggt tttcttttat gtactatttt ttcatattta tgttttaata ttttaggata
     6781 taatttgact tcattttcaa tatttatatt tataattata ccactttgtt ttttgttaaa
     6841 tattcaagat gttattgcta tgtgtgtagt catagcatcc cattactacc ttcaaggaga
     6901 aacttctatc aaatggattt taaatgaagc tggaatattt gcagtaggaa ctggaatagg
     6961 tgtcttaata aatatgtata ttccaacaaa tatacataaa atccatgaag ttcaaaaaaa
     7021 gttacaggaa gaagtgagta ttgtacttat agacattgca gatattattg taaatcctaa
     7081 aaaagaaaat ggatatagta gagatttata tacattaaat tcacttattg acagcagtat
     7141 ttctgaaact tacgataata ttaataatac tttattaagt gatactagat tcttcttaga
     7201 acatatggat attataaaaa gccaacggga tatcttagaa aatttgtata gttatgtatc
     7261 tcaactaaat tctacaccac cacaagctca tattctatct gcttttatac ataagatagg
     7321 ttatactgaa tttgaagctg aaacaggcaa tcttcttctt gaagaattaa agagactaat
     7381 gcttagtatg aaaaaccagc ctctgcctgt tgacagaaca gagtttgaaa atagagctat
     7441 tttattccta tgcttgactg agttaaaaca gtttttagtt aatcgtaaac atgctcagat
     7501 acttagagat aataattttt ataaataaaa aggggagaaa tctagcagtt gacctgacta
     7561 caaaatatca gatataatat taaatgcgcc cctagtaaga gggcgcaatg tttataactt
     7621 attctactgt catgattaaa tcattattca tatcgaacat acattatttg accaagtcca
     7681 atattagctt tttcatcagt gatttctatt ttaatagtca catcattttt tttacagtac
     7741 ttcgctaatt ctgcaccaga acgattttta ccactaaaat ctaaagaaaa tggcttatca
     7801 tatttaaaag gatattcaac aggtttaccc tctggtgcta atgcaaacat cccatatggt
     7861 gtgttcataa atcctattac agcaatcgta agatttgatg ggtctaaatt tgattcacta
     7921 cctgtaaatg taattgtatt tactgatgaa ttaactacac tacatctaac accttcatac
     7981 attacatgtt tttcatttat atcttctcct ttttttccaa aatttccaac agcctttgcc
     8041 ccttcttgat gtggtaacat agtgtgcttt ccaggtggtg ctggtttctg tgtctgttct
     8101 ggttttaccg tctgtactat tgcaccaact tttggatatg ctgtcttatc aaatggtaat
     8161 gggtggctaa gactcttgta tatgatatcc tttcctgctg atttattagc atcaatatct
     8221 acatgataca aacgcccatc accaaaacgg ataagattcc actggtggtc atttgatgtg
     8281 ctgtgtacat aataagattc aattcccata gcatcacaca aagctttcat gctcctagcc
     8341 attcctgagc atgcaccttt tttagtaacc aatgtatagg cagtatttgc tccctctact
     8401 gcattccagt caacatatgt tgtgttattt ttaatgtgat tgtaggcaac taaaatctta
     8461 gcatctgtgg tcatattctt tgtgattttc ttgttcataa aatctgctgt tgcttgtgct
     8521 actgagttta gttgggcttg tgacagcttt ggaccatata cgctattggt tccagtgaca
     8581 tagctttttg gtgtttttgc tgttacctct ttggctgtca taccataaac tgtcatactc
     8641 atacctgaga ttaaaactat tgttgtaatc aataacttgg tagttaactt cctaattttc
     8701 ttcatactta atattctcct ttataaaaca ttgttacttc ctattctcta ccattaataa
     8761 aattaattta gtatttaaaa aggtggattg tcaacatgta tatttctcca ttcatacatt
     8821 cgtgaaatat ttaaatttta tggaaaatct aatagatatt ttctagataa tttgtataca
     8881 atacaactcg caaatcaggg tatttgctgt tagtttgaga aaatgatgat gattaataca
     8941 ttcggaatct ttgacctata ataaatacca ttgtcttcaa tcattaataa tggtaagagt
     9001 aaagagaatt atcaataata cagttaaaat caactttgta tacatttcat acattctctc
     9061 ccaacaacat tatacttctt ataatttgta tattcaatac gttttctggg aaaatatagg
     9121 atatatctat aaaaatataa aaaaggagtt gaacttaaaa tttgattaaa aaaaatcaat
     9181 tttaagacaa ctcatttgta ttataaaaat gctatatatt attattaagt aagtcaatat
     9241 tttagatatt tatctatttc ttattgactt acgcttatag cctaacttat ttgtttagtt
     9301 gatatataac ctagttttaa gcattattaa tatttaaata atcaagtatc ctttttgttt
     9361 cttgtagttt ctgttctgcc tcatttaagt tttcttgaat tatagaaata tatattatat
     9421 ctatcaacgt aagcattgct attcttgaag aaatagctcc ttctctaagt cttttttcta
     9481 tatttggtac tactagcttt aaatcactaa tcttactaag atgattgtca ctatatttag
     9541 ttatacaaat gaccttacat ccttttaatt ttgcattttc tatagacttt attacttctt
     9601 ttgattctcc agagtaagat attgctatag ctatatcttc tttatctaca tttgaagata
     9661 ccataagttg tgtatgacta tctaaagagg taaatgcctg tttatttatc cttaaaagtt
     9721 tcatctgtaa atctagtgca actaaagccg aaccacctac tccaaatata tatacatttt
     9781 tagcctttat tatctgttca actgcttctt ttattatatc tttttcattc aacaaccttg
     9841 tttggttgat agtctctatg tttttgtttg taattttgtt tataacacta tctatattac
     9901 aatcatagtt ttcatctaca tatgtgtatt cattttcttt gcccatcaaa tctttagcta
     9961 tctcaatttt taaatcccca aaacccgaat atcccatttt tctagaaaac ctaactatac
    10021 ttgctgtact tgtttttgag tttgacgcca attctgatac agaaagctta cttatagcct
    10081 ctgtattatt aagtatgtaa tcagctattt ttttatctac ttccgttaaa tcttcatata
    10141 tctgttttaa ttttaaaatg caactcatac tctatcacct ctacctattt actataatat
    10201 ttcttcttag caagtaaaca tgctcctaaa tctggagtca acactggtct cactatacca
    10261 aatctcgttt tattcaaata ttttttaagt ggctctaata ttaaatctcc tgacttaaac
    10321 actccacctg aataagatac ctttatcttt gtaccttgtt caaaatgatg ctctaatcct
    10381 attataagcc tacttaattc ataagctgca tcatcaaata ttttactagc agtattatca
    10441 ccttcaataa ctgcttttaa acatatcgta gcaaactttg ctatttctat tctatctcct
    10501 tgtatttcat catttacata ttttattatg tcataatcat tctctatatt aagatgtctt
    10561 tttattaaat catataattt tgtttttgaa caccttccat cagactgcat tgtgaacatt
    10621 tttaaggtag ccattcctat ataatacgct gaaccttcat ctcctaatac atagccccat
    10681 cctccacatc tgtgaagatt cccatcttta tctaaagcct gtgctattga accagtacct
    10741 gcaattatat ttataccttt ttccccattt aaagaacctg ctaatgctat ttctacatca
    10801 ttacctagag tataatttat atgactatat gcattttttg ttgctacttc taatgcatac
    10861 aatacttctt ttatttttcc atatccagca agtccaacaa atgcatacgt tatttcttct
    10921 acatttatct tagcatcttt acaaacttct tccaacccag ttattaataa tttcgttaga
    10981 ttatcaaatc ctatttgatt ataatgacaa gttcctttgg ttatggttcc tactatattt
    11041 aattcttcat ctactaatgt aaatgttgtt ttcgttcctc caccatctac acctaaaaaa
    11101 tacatactat cctcctacaa tatctttaat aattctttca atacttaata tctaaaaata
    11161 accttccaat gctgtttttt taatattgaa aggttatcag tactattatt tattttacaa
    11221 tttttctatt gattttaaag cttcacttat ataaccttct tgtctatcta ggatactctt
    11281 tgcactatcc ttatctaaac cactttttat cataaatata gccaatttaa cgctattatc
    11341 tgcttcttct aagaattttt ctgcaacatc atattttaca tttgtagcta tcattattat
    11401 tctttttgct ctttcaacca acttttcatt tgatgtctta acatctacca tcaagtttga
    11461 gtatactttt cccatcttaa ccatagaagc tgttgttaac atattacata ccattttttg
    11521 agctgtacca cttttcattc tagttgaacc cattattact tctgccccta ctataatgtt
    11581 tattggtaaa tccgcttttt tactcatttc actattcgga ttcatagtta ttgatagtac
    11641 tgcacacccc aattccttag catactccat tgcacctata acataaggag tccttcctga
    11701 tgcagctata ccacatataa catccttaga tgtaactcct ctatctatta tatcttgctt
    11761 tccaagttct ttatcatctt cagcaccttc tattgcttta aatattgctt gatttccacc
    11821 agctattata gcttgaacaa gctcaaaatc cactccgtaa gttggaggac attctgtggc
    11881 atctagtatt cctatcctac cactggtgcc tgctcctata tagaataatc tacctccctg
    11941 cttcattcta tctactatta tattaacagc ttttgctata ctctcctttt gttcttcaac
    12001 agcataggct acagttttat cttcgttatt tattaattct attatgccta atgtatcttc
    12061 tttatctatt tgcaatgtat tcttatttct accttctgta actaaatttt ctaaagtttt
    12121 tattaacata tcatttcctc attttcttaa tattaaattt ttaaacacat tttaattcca
    12181 actttcataa ttatattttt ataataaatt aatattttca taataaatta ttttgttgag
    12241 ggtttaaaat taaacttttg ccatggttta atataatcta atataaataa ttcttctttt
    12301 cttatatatc ctactacatt cgatttacca ctgtttttca tatcagtcaa ggctatttgt
    12361 aactcccctg cataatgacc atattcacta ctttcaatta ctatatctcc tctttttatt
    12421 attttaggag tattaaatag ttcaaatttg tatcctttat atttaacacg agattgagta
    12481 gaccttatca gactgtcaga tatgtcccct ctattaaaat gtatttcttc aaagagtatc
    12541 ttcttctcaa cttctcgtat attttcctct attgctacat caaatgtaac catatctttt
    12601 ctcatagaac ctaatacctt tagctcctca tatgttggat aacaatttga tattattata
    12661 tcatttatat tattcatagc tattatatgc tttacttgaa tatctatagg tagatttctg
    12721 tgcatctcaa ttgttggaag cccatcagtt acaggccatg gtccaaatgt agtttcattt
    12781 tgacttgtta caaaaactgc tgttcttaat ccatacttat taaatctttc tgtacatttt
    12841 ataaagtgtt ctaaatttaa tccactatat ctatgtggat aaaagttatg acatcctgtc
    12901 aagttttctt tatttggttg gtagtccatt attgtatcta tataattagt attattactc
    12961 atattgattt ctattttaag attttgagga ttaaaagtca ttaatgcctc ttcagaacct
    13021 gtaaatccta tatcaagcct tattccgtct gccttcgtct ttttaaaaaa agtcaaatct
    13081 ttgtagctta tttctaattc accaaatact ctgggattta catcaagtat tacctcaaaa
    13141 cctttaaact tagcgtattc atttatacgt ttaaattttt taattatatt ttctttagta
    13201 tctttaactg ataataaaca tgaaaatata cgtgtaaatc cattttcagc agctttatct
    13261 atataatcat atatttcctt agatgttgtt ttttctggat aaattgatat tcctaatctt
    13321 cccatctgtg tcatctcctt atatcctata tgtgtttaaa tttatttcga attacacgaa
    13381 acttttttaa aaatttggtg aaaaaaagtt tcatatatat ttataaaaaa agaaaagttt
    13441 accttttctt tttttataaa caatcaacaa aatatcttcg ttacttattt cataactgtt
    13501 atactttgaa atttatacta atctaatata gtttttaatt tccaatatag tttctaatta
    13561 ataagcttct tgctcttcaa ctttttcttc atgaagcaat ctcttatcgt acatcttgac
    13621 aaatggatag taaattatta ttgatatagc tatacataat aagttaagaa ctgaagctct
    13681 ccaatctcca ccagtggcta aatatgcccc tactggtcct ggtaatgtcc aaggagcagt
    13741 aactactact ctggatacta aattagcact catagcaaac catgaaactg ttgccattac
    13801 tattggtgct acaacaaatg gtattattaa tattgggttt aatactattg gtgctccaaa
    13861 tattattggt tcatttatat tgaatattgc tggtattaca gttgcttttc ctaaagactt
    13921 tccataagct gattttgctc taaatgcaag aagtaaagct aagcctattg tagctccaga
    13981 acctccaatc catataaacc attgaaaaaa gggttcagca gcaatacctg gaactgcttg
    14041 acccgcagca tatgaagctg tattagcatc taataattgt atccataaag gtcttgccaa
    14101 tgaacctatt attgatacac catgtattcc aaatgaccaa aaaaacgtta ttaaaaatat
    14161 tagtataatt actgatggta atgtgtcagt ggctgaaact aatggagtta ctaatttagc
    14221 tatgaagcca tgccaatcaa accctaggaa ataagtaaca atagccatta aaattattat
    14281 taatgcggct ggtgtcagag cttcaaaaga ccttgtgact gatgctggaa ctgcttctgg
    14341 catggttatc ttgaatttag atttgtcagt aaatctataa atctcaacac ctattataga
    14401 tgttacaata gcaacaaaca ttgaacctcc accaagatta gccattggca tttgccatcc
    14461 ttgaggaatt gaattatacc aagctaaaag ttctggactc ttttcaacta tctttaataa
    14521 ctcaggggta agaccctcta ttgcagtagg aactattgtc aaaaggaatg tagctgtagc
    14581 taataaacct cctgtcaatc catctaactt atatgacttt gccaaactat aacctatgcc
    14641 aaatgtagaa tatactgata taataaaaaa tgtcattcta tatggtagta atattgtaac
    14701 tgcattttcc tttagatact gagttacccc ccatgaatct ggtaatggag gaaatgctaa
    14761 tatcaagaaa aatgacccaa ctattataag tggtaatgaa gctacgattc catctcttac
    14821 agctcttaag tgacgttgtt ctgccaatct ggccatagaa gttgataatt tagtgtctaa
    14881 aaattctgta aatttatcta gcatcttttc tctccttaat taatatttat ttttattatc
    14941 attaaaattt ttataaaatc aactttcttc taatctaaca acactgcaac tactatttac
    15001 tcaacagttc ttgaacttgt tttaataact ttggtcctcc taatggacta taagcttgtg
    15061 cttgtattaa tgcacatggt acactaacct catcagcaaa agcttttaaa tagtcaaatc
    15121 tatgtctgac ttgtggagct accatagcaa tatcccatcc atttttaact tcattctcaa
    15181 actcttgagt accaacagcc aaaacatcta tatctattcc attttttgct ccttcttttt
    15241 gtaatgcatt aactgctata gcacttgaca tccctgatga acaaacaaat aatactctca
    15301 tattgattcc ccctgttttt ttatttcttt tcaatcatta tagcatgtat ttttgattac
    15361 aggaatacgt ttgtatatat tttttcatat tttacatttt aaattgtaat tttatttcat
    15421 gcatttatat cttgctttaa ttgctttatt tatctaatca ttcttttgtc taaaattaca
    15481 cactacttaa aatactcata tcttttctta aaaaagaaaa aagactatct tattattaac
    15541 ataaaatagt ctcaatactt ttttaaatat gtattccttt atatatccag aagtaaccta
    15601 ttattgcaaa tgataaagca aataaaatat acagtctaaa aaatcccaaa gaaaaacctc
    15661 tcttagtttc aacaaagtat cttgtatatg caactgcaaa agatgtaatt aatgaatttc
    15721 ctataacaac tccaaatcta ttatctattc caaatataga taataacaaa ggaaaaacta
    15781 cagtacttac aagagatgaa actattagta ttaatccact tttaaaactt atagttggaa
    15841 tattataatc tgttctttga tttttctcca tttgtttctt agtattttta ttactttttg
    15901 tactttttgc atttttataa ttattatttt tctttctttt ctttttttta ttattagaca
    15961 tgtcatttac ctccaaaagg tttaggtgtt atattcccta aaataacttt atttttagcc
    16021 cctgttgcag atattacatt agaaaaagaa ttatttaatg tttcattgcc caagattaca
    16081 aatgccaaag cctcctttgc atcagaagaa tacccatatt catcttgagt taatacttca
    16141 acctctaata ataactcttt tatatatcct attaaagtta gattatgagc tcctccacct
    16201 ccaattaata aagtatctac attaaccttt ggaagtatga agtttttata attataatat
    16261 atagcttttg ccgtaaacat agttactgtt gctatcaaat catatttatc tacatgttta
    16321 tatttgttaa gcatgttatc caaataaact tgtccaaata cctctcttcc agtgctttta
    16381 ggtggcgctt gactaagata cttatgactc atcaaatctt ttaacatttc ttcattaatt
    16441 ttaccttttg atgcaaagta tccattttta tcatactttc tattaaataa tctttgacat
    16501 actccatcta ttatcatatt tcctggtcct gtatcaaatg caaatacatc atctatgtta
    16561 catgatttag gtattatagt aatatttcct attccaccaa tattttgaag tgctaaattt
    16621 ttattgttat cactatataa taaaaattca ctatatggta ctaatggagc accttctcca
    16681 cctgctgcta tatccattac tctaaaatta gatattacct ttgtatttgt ttcatatgct
    16741 ataactgctg gttcacctat ttgaagcgtt gaagatacaa aattattata acttcttggt
    16801 atatgaaaga ttgtttgacc atgagatgcg ataaaatcca aatttgctat atgaaagttt
    16861 gctttatgac acactgattt tacagcttta ctaaataaat atccaagctt aaaatttaag
    16921 ctacagatta aatcaacact cgattcctct tctatacagc actttcttat ttcgtctttt
    16981 atatctttag gaatttctag agttataaat tctatttgct taactttagt atttctacca
    17041 ttcccactaa tttcagctag tactgcatca actccatcta aggaagttcc tgacattaaa
    17101 cctacactat acatagttat catcctttta agtttatagt tattcctccc caaatcaagg
    17161 tattaagttt aataatttta gaattaatct tatttcatct ttaattacga taatttactc
    17221 ttacgaaatt aatcgagtta atcttataca cgttttttac cttagctaaa tcttacatta
    17281 atttattttc agtgtcaatg taataccatg taattttttt tcacaaacaa aacatattct
    17341 ctctccaaaa accatattaa aatagttact acaaataaaa aacataccag gtacctatct
    17401 gatatgtttt tcatatataa taattattat tttataatat agatggagca tcatttaagg
    17461 cactaattaa atcttcacta ttttcaagaa ctaaatttat tcttattgta tatgtcgatt
    17521 tgtatatgat gatagatttt atagtctaat aacctaacta tctatgctta aataatccga
    17581 tatatcataa aagtagatat taaaaattgt atatgaacca attttcgaac atacctcaat
    17641 ctcatgccct aaactatcta tcattttctt tacaaaatac atccccattc cagtagactt
    17701 atacaattga tttcttccat ttttccccgt aaatcccttg tcaaaaaccc tagatatatc
    17761 ttcctcattt atacctatac caaaatcttt tatagataat accactccat tttctaactt
    17821 ttttgaatta aattcaatct taccaacttc ctttgaatac tttatagcat tattaataat
    17881 ttggtctaaa acatatgaca tgcatttaga atctgtgtaa acatcatttt ctaattcatt
    17941 caaactaact tctacattat tttttataag caaaaatgaa ttatttttta tagacatttt
    18001 taccaatttt tctaaattaa atttatttat aaatacatct tcaaacatag tgttgcttct
    18061 actactatac aaaatagagt ttactaaaaa atttatcttt gcaacctgat tcttaatatc
    18121 caaactatct tctatgctac ttaacctatc tgtaattata cttaaagaag atattggaat
    18181 ttttatttca tgaacccatt tagctatgta ctcatccaat tcttttaatg attgtttata
    18241 agtctctatg tcactattat tttttatttt taagttttct ataatatctt ttattagatt
    18301 ttcttcaaaa gattgtccat caactaaata actatctatt tcaccactat tttctataca
    18361 attatataaa tttgtgtaac tatttctaaa atttatataa tctataacaa aaaataccat
    18421 tattataaac agcactaaaa aatctaaata tattaactct gaatatttat ttttaaaaat
    18481 atctatagca aataagtaga tatttattat actaactaca actaaaggta gagcaatata
    18541 cttagaccta cctttcaaat attttactag cattgtatta tatatcctat ccccttctta
    18601 gtttttataa aatcatcaaa acccagttct ttaactttac ttctcaatct attcatattt
    18661 acagtaagtg cattgtctgt cacaaactcg tcattatccc atagagtcat cataagactt
    18721 tcccttgata caaccctatt cttatttttt aataataatg ttaaaatttt tatttcgttt
    18781 ttagtcaact ctatactttt atccttatac ttatgtttta taatcccttt ccccacatca
    18841 aacatcaaat cttcacaata caaaatacta ttagaatcac tataattata gactcttctt
    18901 attataccat ttattttggt aaccaatatt tctatagaaa atggtttggt aatgtaatca
    18961 tctgctccat tatttatgcc cattattaaa tccatatcag aatctcttga tgataaaaat
    19021 attaatggta catttgaaat agctcttatt ttttcgcacc agtaaaatcc attatataat
    19081 ggtaaattta catctaaaag tactacatct ggttttttat ctataaattc atctaatact
    19141 acctcaaaat catcaattaa ttctacttta aatccccatt tttcaagtgc taacgcaatc
    19201 tcttttgata aatcaatatc gtcctctact aataaaattc tatacatttg tatattctcc
    19261 attatcctaa tatttcacta tgctatgaat tattaatatc ataacatatt ttatatgaat
    19321 ttattaccca aattatttag ttaatcaaca aggagactct tattacaaga atctcctttc
    19381 tctttattaa tatatcaact aatttttata cactacactc ttaaacttat taacagatac
    19441 tgctccatat attatgaata ttatcaaaca tatcactaaa cttagtatat atataccaaa
    19501 taaatcggtc atcaataaat ctcctgctaa tctcaatgca aaataactag atatagttgc
    19561 acttaacatt ggcaaagtat aaaaaatagc tacttcttta agtatagctc tatttatatc
    19621 tttttctgta gtacctattt ttaacaaaat cttatatttc tctttatctt catatgcatc
    19681 actatatatt ttcatatata ttatacttcc agctacaatt gcaaagacta agaataaaaa
    19741 cacaagcact acatatgcaa acttcatcca ttgactctca ccttgagctt tatatccatt
    19801 ttgaatatac ccatttttta aacttaaatt cttttcaata ttgttgaaca ttacttgact
    19861 attttcttct tgaaatacct ttgcaccata aaaatacact tgttgaccct tatctcttag
    19921 tttaaaatat gtatcatcat ttaccactat aatactttga ttaagtcctg tccctagtac
    19981 tggaactttt acttctcccc ttgaaatatt atataactga ccatcaagtt ttatcttggc
    20041 ttcttttgta gctattgatg ctatcgtttg agggcttttt aactctatta cattatttcc
    20101 actaactaat ctttcaccat actcttttaa ttctttatca ttaccattca ctttaagagt
    20161 attacaaaaa tcactatatt tcattatagt atctgttgta tcataagctt cttttatatc
    20221 tgttttcttt aaaattgtta gttctggatt tactgagtac tttatcttat tagtatcttt
    20281 tattatattc tctatttgtt ttttattcac ttcatcttta tccattgaaa ctatgctaat
    20341 agtgtacaca tttctttgtt cttgggcatt cttatgtata tgcttcatag caactgaagc
    20401 accaagagtt gatattgttg tagttataat tatagctatt atagcataag ttttatagtt
    20461 tttatttaat cggtagttta tattgtttat tgcaattata ttatttccat taaataatat
    20521 ttttttattt ttgattataa tatcaaatat aacaggcata actgatttga ataatccata
    20581 agtacctact attattatac ctataacagg tagtacacta gttatatctc cagttcttat
    20641 attggtagaa agatagtagc catatcctat tactataatt gatactatag ctaatatata
    20701 tatacctatt cctacctttg gtattttttc ttgtttttta gatatattga ttagatttat
    20761 tacactactt ctacatattg ttataaaacc ttttacagcc attattataa atatagaaat
    20821 aaagattaaa gtagcatata gtatagcttc tatactaagt ccattagata cctttatatt
    20881 aaatccagat aactttatta tcactacttg aaaaaatcta gatgttatta tccctataat
    20941 tattcctgtt atacaagaac ttattcctat aagcatattt tctacaaaat atatttttcc
    21001 tatagtatat aaatctaatc ccataaaaga caatattcca atttctttag acctattctt
    21061 aaaaaatata tttgttgcat accatataaa gaaaaataaa aatactgata gtatcattat
    21121 tacagctact aacactgata gtgtaaattt tttattttcc tcttgtaaca catttatagc
    21181 atcaccatat cttaataatt caaaattagt tagcacaact actgaaaaaa ccatagatat
    21241 aaaaaatgct agataagttc ttatgttact cttaaaatta ttataagcaa ttccaagtat
    21301 actcacttac atctcacctc ccatagaggc tagcatatcc ataatttttc cataaaattc
    21361 ttttctacta tttcctttgt ttaatttaca ctttatattt ccatcattta tcatatatac
    21421 ttcatttgaa taacttgctg ttagagggtc atgtgtaatc attataatag tagcgttatt
    21481 ttctctgttt attttttcta atgattctaa aagttcatat gctgatttag agtctaaagc
    21541 accagttggc tcatcagcaa atatgactgt tggatttgta attaatgacc tagctgctgc
    21601 gcctctttgt ttttgtcctc ctgataactg atatggatat ttatctaaat gattttctaa
    21661 tccaaatttt ttagctatag aaaatacctt agcttctatc tccttacttt tcttttttcc
    21721 taatgctaga ggaagtgcta tattgtccat tagtttcata ttgtctaata gattatagtc
    21781 ttgaaatata aaccctattt tttctcttct taattttgat agttcattat tttttacttt
    21841 tgatatatta attccatcta gtattatttc tcctgaagtt tgtttgtcaa gtgtagacat
    21901 taaatttagc aatgttgtct ttcctgcacc agatggaccc attattgata taaaatcacc
    21961 ttcattgact gtaagagata tattttttaa tacatttgtt ttaaatcctt ttattccata
    22021 atcttttgat atatttttta tttttaatat ttctttcata acaatagcct ccagttgcta
    22081 attttttaat atatatttat tttaaaggtt ttatataaat aaatccttaa gtaaatatta
    22141 cattttaact gttagcatta cacttttgta acatatggaa gactattata attatagtag
    22201 aaatatctta aaataaaagc atgatttact tctttgtata aacaaagaaa agaagctcct
    22261 aagaagcctc taatctatac aattattctt ctctataaaa aaccaaaata cacattgatt
    22321 gtggcaaata cagatactat aaacaagtga tattactact atatattaaa acaattattc
    22381 ctgaagataa ttcaataaga ctatcaagca ataagctaag catccaaaaa gacttaatac
    22441 cattaatgta taattatacc caatattaat gcttaaaaaa ccagcaacag ttggaataat
    22501 cagagatgaa cctcccataa atatacttaa agctattaat tggctactac ttctaagttt
    22561 tttaggagaa acttcaaata ttaatctttt tgaagtaatt aaaattaaag gagtagaaaa
    22621 cacttgaaaa acacacagta taataatgat atttgaatca tttgttaaag caaataatag
    22681 aaactgaact gttatcataa tagcacttaa ttttaataag tttaaactgc tatatttttt
    22741 taataaataa gaaccagcca gatatgttgg tatctctaac aatgactgta aagaccactt
    22801 taaagatatc tgagaatttg tagcatttaa agctatcatt ttatctatta cagtacagtt
    22861 atttgctacc accattgagt ataataagaa taaaatacat actagtaaga tatatttttt
    22921 atcttttaat aattcaataa tatctttatt agttacattt attttcttat taatcttttc
    22981 aatatcaggt aataatagta tatttataat aagtaatact acaattaaac caatcgctac
    23041 agataatcca aaatatgaga atctaagaat caagtaagat gctaccatac ttccaatagc
    23101 ccatccaatc gacccaaaag tcttaataaa tgacaaataa ttattaatat tatctccaca
    23161 acctattatc catgtatcat ataaactaca gcaagtattt aataatcctc ctgataaagc
    23221 aacaagtaat aagtatatta taaaagttgc agattcagta gaaaacataa taactgatgc
    23281 aattccataa actgataaac taattaaaac aatttttttc attgtttgat acttatcagc
    23341 taatactcca aataataatt gaaataatat attggttatt gcataactcg ataatataat
    23401 tcctctttca aacgctgagt aacctaattt tgataagaaa ggtgtgaatt ttgttgaagc
    23461 aaaggcaaaa ataatatatg agaaaaaata gaatatgata aatttaaatt taatcgcttt
    23521 attaatcata atcaatcaaa attgataact ttaataccac tatttccttc ctgatttaag
    23581 tttgaaagat gatattttac cgtattagga ttctctacaa taactaaagg aatatataaa
    23641 gtattttcaa gtaacttatt atcaatttct actaactttt cccccatatt aacttcttga
    23701 ccttgtttaa cttctaattt aaagttttct ttttttgttg ttgcactgtc caatccaata
    23761 tgaataagaa tttgaacatc attttgcaat ttaattccta tagcatggtt agtttgagca
    23821 ataaaactta ttactccttt agtcggtgat ttaagaatat tgtcctttgc tatcatagca
    23881 aatccatctc ccattacctt agttgaaaat actttatcag gaatttctcc aagattaatt
    23941 atttttccat caatgtatga attaattgta attgcttctt tttttctttt aaagaacatt
    24001 atagacactc ctctacataa ttaactttat tctaattatc attacaaata acgatattaa
    24061 gcatactgca attgaggaaa aaagagcctt atagctcttt ccccattgta gttctttaat
    24121 actgcttatg atttgtttaa ttccaacaaa tttataaata taggtaaaca tgataacaat
    24181 agttattgtg taaatttgga atatctgccc taataagact aaatagaaag caatcatgtt
    24241 ttcttttatc tctttattct tttttatatt gtaaataaca tatgcactgt atactgcact
    24301 aaagttagag attaaattta aacttgcatt aacatcattg tttaaaagtt gtgaaaatga
    24361 aatatttgta aataatgatg tcaattctaa atacactata tataaaacag gtaatattag
    24421 aaaacaaacc ataaccactg aaagaaatat attttcatct tttaatactg ctgtgatatt
    24481 ttttatttta tttgttatca tgtttttgtt ctccttattt aaaagttggc cagaaatcct
    24541 tattagccac aatcatttca tctaagattt cttttgctac ttttgcactt ggaacagttt
    24601 tactcatagt aaacgactgc cataattttt gataactttt ttctatccat gcttctacag
    24661 ctaatttttc agaagcaact tgttgttcca ttaaaccttt ttggaaagtt ggaattttac
    24721 ctatagacat aggttcaacc atatcttttc caacaaggca aggaatttca accattgcat
    24781 catctgcaaa attttcaata gctccattat taggaacaat cagtaaaaat ctttcctgag
    24841 tattaaatgc taatgcacaa gctaattcaa caataaattg agaatgctca cttgcatcaa
    24901 accatgtatc ccttgctgtg ccatttttta taattctttc acattcttcg aatgtatcct
    24961 tttctcttct atccataatt tcatttgctc ttgtataatt tggattagca tgttcaacag
    25021 tttcatcagc cattaaataa tattttaaat aagtattagg taatgtagtt ggttctattt
    25081 ctaataagtc ctttgctgct ctcatagttt ctaaccaact agattctttg tgttgacctt
    25141 gaggtgtaaa ttcctcatat ccttttttag cacaatattc ttgtaattta ggcattaaat
    25201 catttccttc tttgtcctta atacttgtcc accacccgaa atgattcaat ccataatatc
    25261 taacatcaaa gtctttacga gaatttaaac ctaaaactct agcaaatatt tcctctaaac
    25321 atactggcat atcacaaata ttaataattc ttgaatttgg tcttaataca cgacatgctt
    25381 ctgcaactat agctgctgga tttgaataat taagcatcca agcatttgga gaatattttt
    25441 ccatataatc aatcatttcg attattggac ctattgttct taatccatag gcaattccac
    25501 ctgccccaca agtttcttga ccaacaaccc catattttag tggaattttt tcatcttgtt
    25561 cacgtaaagc atataaacca acacgaattt gtgccattac aaaatcaata tcagtaaatg
    25621 cttcttctgg atttgttgta taactaaatt taatttctgg atttttctct ctaacaatta
    25681 ttgcacatgc ttcagcaact tttctttgtc tctcatcatt attatcatat aatttaattg
    25741 attcaatagg aaatttagca acactttcaa ttaacattgt tacaattcca gcagtataag
    25801 tactaccccc accagcaatt agtatcctct gtctttttct ttccatcttt ccttctcctt
    25861 tataataaat tttcaaattc ttctcttaca cttggtactg aaagcccaat aataatctga
    25921 atagcttttc cattatgcac taaaccatgt gcacctactg ccttaaaatc ttcttcttta
    25981 gaaataattg agtcatcttt caaggttaat ctcaacctcg tagcacagtt tgtaacatct
    26041 acaacatttt ctgctccacc aactaatttt atgtattcag atgcttgttg agaggcttta
    26101 ctcaatttat ttcctttttc atctttagtt tctaagtatt catttttagt atatagtttt
    26161 gtttcttcac tatcttcttc tctaccagga gttttcatat caaacttctt aattaagaaa
    26221 gtaaatacaa agaaccagat aaatgaaaat ataatcccta caacaacttg aacaacatat
    26281 accttccagt gatacattcc aagaggaagc caatttaata caacccagtt aataattcct
    26341 ccaccaaaat caccaacaac tcctaaggca aacgaaatcg tttgcaagca cgcatctaaa
    26401 aatgcatgta ctaggaataa aagtggtgct acaaataaaa atgtaaattc taacggctca
    26461 gtaattccag ttaatactgc agtaagagta gcaggaatca ttaaacctaa tacttttttc
    26521 tttttttctg gctttgcagt tacatagaaa gcaccaaata cacctaatga accaaacacc
    26581 ttcgataatc cggataatga aaatccgatt ggatacattt cctttagagt cttagctgaa
    26641 gtttgaaaat cctgaatatg agtagcccaa tatactgatg taccacctgg aacaacaaca
    26701 ctatcataac aaattggtgc atatacaaaa tgatgtaatc cagttggtat taaaatcttt
    26761 tgtaagaatg cataacacca aactcctata tttccactat ttataaaaaa cgtttgcaaa
    26821 tgtctaataa cattttgaat atgaggccaa ataaaacaag ttaataatgc aactggtatc
    26881 ataacaaaga aacaaattgc aactacaagt gatgagcctc taaataatcc aataaaatct
    26941 ggtagttctt tatcataaaa tctattatga atataaatga caatacctga tattaacaaa
    27001 gcaccaacca taccagtatc taatgtttta ataccagcaa tgcttacaag tccacttcca
    27061 gcacttattt cagctgcgaa atcaactcca aagctttttc cccaaaaact taacatagaa
    27121 cctacaaaat aattaaaagt caaatacgtt gctaaagcct ccatacaagc tcttgcttgt
    27181 tgcttttttg caagtgctat tggtaaagaa atagcaaata ataatggtaa ttgattaaat
    27241 accatccaag ctccactaga tattacaccc caaaattggt accaaatatt ttctggtgta
    27301 gcaagtgaac ccataattga ctgattttga aatacaattg agaatccaac cattatccca
    27361 gcaaaagcaa ataatagtgt tggtgtaaac attgcccctc caaaacgctg tattttcttc
    27421 atcatataaa ttactcccct cctcatattt ttagtttatc tttggcctta gatatctatt
    27481 attttaacag gttaataaat atatacaatc tttagggtac atttggagct tattcacagt
    27541 caaataagtt tttgcacatt tttatgttca aattctcata aaaaaaaaga atgactacaa
    27601 tttgcaatca atcccttctg taatcattct atgcctttta taaatactat atttaataaa
    27661 taataactca atcaatataa aatataacat tgtcaattta taactagggt gcatttgtaa
    27721 aaatgagata ttagtcgctg aaatataaat tgattcatca cttaaatcag tcaatgtatt
    27781 atttttaaac tctgtaattg aaattatttt agttcctatt aattttaatc ttctagcaat
    27841 attaagtgta ttctcattct caccacttag ggatataata atcataacat catttttact
    27901 gacaatattg taaaaagaat catcaaatgt tcttccctca aaatcatata ttatttcttg
    27961 acatgataag aacattctct ttatctgttt tgcaactaca ttttgaatat cacctgaacc
    28021 ataagcgtat attgttcttg attcataaag aagtttacac aatgcatcat aatctttatt
    28081 tttttgatct tcaacatact taattattga attacatgca atatcaaatg actgattttc
    28141 tatgacatga accttttctg attcccagct taataatact ttaaactcac tgaaaccttt
    28201 taaatctaat tttttaacaa atcttgtaat agttgcacga gacacattac atttcatagc
    28261 aagttcattt attgacatct tactacaatg ttttttattt gcagttatat atttccatat
    28321 taataaatct gtatcattca atttattata atttaaaaaa atcatttctt ctaatctcat
    28381 aaactattac cccctttaat taaaaatcgt cataaatatt ataccaatat tcaacattaa
    28441 attacagcgt attcttatat ttactattta gcccataaaa ttgactataa aaaatactgc
    28501 tataattgat gtttaaaaaa atgtagtttt aattttattc ttttatctaa taattaatat
    28561 tttcaagttc tagtgctttt cttatacaat tatcatcttt aaaatcagca gtccttttac
    28621 aatcttttac tttaaattct ggaatagttt taaattcctc atcacatatt ccattcttat
    28681 ttaaatacat acaacttgcc attctaacta ttagaccact attttttaaa ttaaacaata
    28741 cagggtctat acaccctcca tctccgccag tagtttcacc aactaaagtc gcaaatttag
    28801 tttctttaca aaacattgca aatgactctg atgatgaata tatttttcta tcaacaagta
    28861 agtatatatt acccttaaat tttgaatcat ctgtagattt tatagtatag gaagtatctt
    28921 caaaatcatt aaaatctttt ataatttctt taggtgcatt ttcctttaca cttgttggta
    28981 aattttctac aggatttaat tttatatttc ttgcatttgt atattgtttt actatttcat
    29041 tattatttct ataaattcta taaccattta ttttgacatc attttttatg agtttagata
    29101 cgattccctg ccaataacta tcacttccac ctaaattacc tcttatatca ataattaaag
    29161 ctttatgttt ttctaaagtg tcaatataat ctccaattat ttttaaatca ttatttatag
    29221 agccattctt tgatgacatt gatggtaaat acatatatcc aatctgaccc ttaataacat
    29281 cctttaaaat taattctttt tttacaaaaa tatcatctgg catttttatt ttaagattca
    29341 tagagtcata tctatctaca acttttttat cattaagaaa atcataccaa tcattattag
    29401 agtatgattt cttaaaaagt tcatatcttt tcttattatc tattacttct gtgtgtttat
    29461 tatttaaatc agataatata gatgacattt cttctataaa ttcttcatca ttttcagtgt
    29521 tttttattcg ttttgtatat tcatccttat ttgctaacca atcaatatta ttaagcctct
    29581 tatttacacc tagatatgga tatcctgtct ttacaacatt atacacataa ttaaagtcat
    29641 ccaccttttc ctcttcagac aactgattct tttgagaata agtagaatta cctaataaga
    29701 ttataaatgc taaaataatt actaaaaata atactttctt tttcatgtaa ttctcatcca
    29761 ctttcagatt ttatatttta atacattgta tcattatttt attatcacct aaacagttaa
    29821 aagaaattct tgaagatata ttccactatt attctcttta aaaattttta tttgtatttt
    29881 caaaattatt tcatgaaagt tataaaataa caattatatt caaatatcta ttt
//
LOCUS       DAEPIN010000053        28279 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.053, whole genome shotgun sequence.
ACCESSION   DAEPIN010000053 DAEPIN010000000
VERSION     DAEPIN010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 28279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28279
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.053"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            <1..907
                     /locus_tag="KR250_002540"
     CDS             <1..907
                     /locus_tag="KR250_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HBH0068469.1"
                     /translation="LEGFNNVSTISNNIILYTWIITVVVYLIYTIFKYIKLKNLINDL
                     SYDVDDREIVNLYKNILEEFNITKDIKLKYSYEVETPAFFNSCVLLPPHEYKLKELDW
                     IFRHELMHFKSRDLYLKYVILFLKTVYWFNPFIYIMDKHIDLDCELYCDERVLKNRNS
                     EEKKDYALTILNAMRKGSNTSNKFIAGLHKQSDIKKRVMNMFNEKYKNGILMALTLCL
                     LSSITFLKVNSTSIVNLNVLPKGFEDLRTVSSIRFSVIGFTYADAPKNMREEYKINCE
                     SLGLIPKDSDKIEVGIEYYDSLLND"
     gene            1823..2845
                     /locus_tag="KR250_002541"
     CDS             1823..2845
                     /locus_tag="KR250_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBH0068470.1"
                     /translation="MKKKILIPVFASVMALSVSSIVNADEVNDSSQNKDDKTNTELNL
                     GEYKEVKYKVAKIKDGVAIKIREEGQVQNIAYSGDEFTVLGTQGEWVKVKVEDGEGWL
                     ATRYVDISEGVGYTNADKVNLRKDKSESSEVIEELEKGSSLLVLEDNGDWLKVKDGET
                     EGYVKSSYISDKAPVIEEPQVNPSIDQNVDSTNNNQQNNANTNQQNNNSNVPTANSNA
                     VQAVLNLAYSKQGCPYVWGAEGPNTFDCSGFTQYVYRNAVGKNIPRTSKAQSKYGQTV
                     SKANLQPGDLVFFTTNGSGSVSHVGIYVGGGNMIHSPSTGKTVSVTSINSSYYTARFV
                     TAKRIL"
     gene            3142..4062
                     /gene="pyrB"
                     /locus_tag="KR250_002542"
     CDS             3142..4062
                     /gene="pyrB"
                     /locus_tag="KR250_002542"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HBH0068471.1"
                     /translation="MLKSRNLIQPEDFSIEEIDEILELAQKIIDNPSKYSRICEGKLL
                     ATLFYEPSTRTRLSFESAMNRLGGRVVGFSEPNSSSASKGETLGDTMRIVSGYVDIIA
                     MRHPQSGAASEAARYTEVPFINAGDGKNQHPTQTLTDLLTIKSLKGTLESHTIGLCGD
                     LKYGRTVHSLVKAMARYKNTKFVFIAPEELKMPDYIKEAIKGHAYYETNNLDDVIGSL
                     DVLYMTRVQQERFEDKSEYERLKNYYILNKAKLEKASKDMLVMHPLPRVNEIDIDVDS
                     DDRAVYFKQAKYGMYVRMALIIKLLGINED"
     gene            4101..4793
                     /locus_tag="KR250_002543"
     CDS             4101..4793
                     /locus_tag="KR250_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HBH0068472.1"
                     /translation="MYKILENIYIGEDMYRMKVKGNFEGKMGQFYMLRAWDTYPVLSR
                     PISIHDIDEEGITFLYKVVGEGTQILSNLKVNDTIKLEGPYGNGYAKVDGKVALVGGG
                     IGVAPLYLVAKNIKNCDAYLGFREDVILEDEYKQVCNKVYTTVGNTFVTDIIDVEKYD
                     YILTCGPTPMMEKLVKMVEGTKTRIMVSLENHMACGVGACLVCTCKTNGGNKKTCKDG
                     PVFWGEDVIFNG"
     gene            4786..5688
                     /locus_tag="KR250_002544"
     CDS             4786..5688
                     /locus_tag="KR250_002544"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HBH0068473.1"
                     /translation="MGNLSVKFGSVEFKNPVIMASGTFGFGKEYNEIYDIQKLGGISS
                     KGLTLNKKPGNNGMRVHETPSGMMNSVGLENPGVQGFIDYELPFFSKLDLVRIANVGG
                     GTLEDYLLGVQMLNDKPIDIIELNISCPNVKAGGMAFGIKNEVAREVVREVRNITKLP
                     LVIKLSPNAEDIVGMAKVCEEEGADGVSLVNTFKAMAIDIKNRRPVFENVYAGLSGPA
                     IKPIALRMVHEVCKNVNIPVMGMGGITKATDAIEFIMAGATCIQVGTANFMNPRIGIE
                     IIDGINEFMDREGIKSLDEIRGII"
     gene            5773..6357
                     /locus_tag="KR250_002545"
     CDS             5773..6357
                     /locus_tag="KR250_002545"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HBH0068474.1"
                     /translation="MSKVDVVDILKKSDALLEGHFLLSSGKHSNRYVQCAKVLRFPEY
                     AAEVLSTVVEQIKDLDIDLVVGPAMGGVIVSYELGRQLGKEAVFTERKDNTMELRRGF
                     EVKKGAKIIIAEDVVTTGKSTMETKRVLEALGGEVVGVACIADRTNHDIGMPIYSAIK
                     LDIQVYESDECPLCKEGKLPVVKPGSREFKELGM"
     gene            6572..7498
                     /locus_tag="KR250_002546"
     CDS             6572..7498
                     /locus_tag="KR250_002546"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HBH0068475.1"
                     /translation="MKNQLNENHKKVVFFGVGAVGATFAEQFFNSKYDFKILCDNERK
                     KRYLEEGFIINGKRYDFDYVTKDEYKQEADFIIIGLKYNNLKENIKELDGLVGKNTVI
                     MSLLNGVDSEEIIGERFGIEKMVYSYVTNIDAKKINNNIIHTTNGIIVFGNKDNSEDR
                     KTNIITEVFDDVNIEYTLSKDIQRDMWWKYMVNIGVNQTSAILGAPYGVFQSSEHLRE
                     LAKSAMREVVAIAQAKDISLTEDDVEHSLHRILEHSKEGRTSMLQDVEAHRLTEVDMF
                     SKNICKLGKKYNIPTPINQTFFYMIKVIESRF"
     gene            complement(7550..8440)
                     /locus_tag="KR250_002547"
     CDS             complement(7550..8440)
                     /locus_tag="KR250_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBH0068476.1"
                     /translation="MFEELKTFVAVVEYKNFTKAGEYLNLSQPSVSKHIKNLENYFKV
                     VLINRSIKQKTIFITESGQILYKRAKEILNLLNITYHDVSQVSDAITGHLKIGASLTI
                     GEYILPNFLALFSKKYPDIDVEVFIKNTSIVSSHVKDYILDIGLIEGTCSSPSFIQEY
                     FFEDKMVLALPYKSHLLKDFSFDKLQNQKWIVREDGSGTRDYLDMFLSVKEIIPKSMM
                     VFGSNYAVKESVRNNLGITIVSNLVTSLPVLNNELSVIELGSSYNRHFSYIFPKDITL
                     SKAATIFIEELKIFSNLNSI"
     gene            8628..9659
                     /locus_tag="KR250_002548"
     CDS             8628..9659
                     /locus_tag="KR250_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HBH0068477.1"
                     /translation="MLKKKSSLTLKTIREILPGLFVSVLVGYISIFISILIPKVGAAS
                     ISIFLGMFVGNLFLNQKVFQKGYKFSETDLLSYSIVLLGATLSVSTLIDLKVSGILFI
                     ILQMTITIIAALYIGKKLGFEENFRFLMASGNAVCGSSAIGATVPVVNATDKEKGIAV
                     TIVNVTGIFLMFLLPIISQFLYSHELVKTSAMIGGTLQSIGQVVASGAIVGENVKDLA
                     TIFKIVRVIFLVVVVLVFGHMKNQSNREILEEEKDEIKKKKVTIPWYVIGFFITCALF
                     SMNIIPQEVSVLCKEISNKLEIIALAAIGLKVNVKDLVKQGKEVSLYGLFVGIVQVVS
                     AVVLIKIFI"
     gene            9953..10828
                     /locus_tag="KR250_002549"
     CDS             9953..10828
                     /locus_tag="KR250_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA glycosylase"
                     /protein_id="HBH0068478.1"
                     /translation="MNVYEKGNGVILEGVTDFDPVHIFECGQCFRWHKQEDGSYTGVA
                     KGRILNVKKENDKIYLNNTNLKEFNSIWYNYFDLGTDYTEIKNKLKNMDEYLNKATEF
                     GWGIRILRQDGWEMLISFIISSNNRIPMIQRAIENLSRKFGKYIGEYEGNEYYAFPTP
                     EELNKASQEEIRACQTGFRDKYIKSTTQAVIENNDEVSEYTNLSTEDCRKELLKFNGV
                     GPKVCDCIALFGMQKYDSFPVDVWVKRVMQEFYIDEDMSLPKMRTYGIDKFKEMSGFA
                     QQYLFYYARELGIGK"
     gene            10846..12336
                     /gene="cls"
                     /locus_tag="KR250_002550"
     CDS             10846..12336
                     /gene="cls"
                     /locus_tag="KR250_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HBH0068479.1"
                     /translation="MGVIGTIFLFYLIISYLAGAIISVIILLENRDPAKTMSWLLMFI
                     IFPGVGLMIYAISGRNIRKRKLFKTQKLANNIKEKKLFDTLEKITEIVELEKESIKQN
                     KLLRDEEDGSYRKRVINMLLKTGMFPFTKNNKVDVFVDGNEKFKRLIEDIREAKDHIH
                     LEYFIIKDSEIGRVLKEELIKKAKEGIKIRILYDDVGCWRFWFNRKFFREMREVGIEI
                     AAFLPTKFPIIGGKLNYRNHRKIVVIDGIIGYTGGINIGDEYLGKNDKFGYWRDTHIR
                     IKGISVYMLQMTFLIDWYYTTKEVLVTKNYFPSVGNVGESMIQVVASGPDSDWEDIHY
                     AYFSAICQARKNVYIETPYFIPDESLLKAIKSAALSGVDVRIIFPKIADHKIVNIASY
                     SYFEEILRAGGKVYLYNKGFIHSKVVIIDDKIASAGTANMDLRSFMLNFEVNAFIYDE
                     EVIRVMTDDFFEDLSHCEELNLEVFKNRNIIQKIKESVARLFSPIL"
     gene            12529..12813
                     /locus_tag="KR250_002551"
     CDS             12529..12813
                     /locus_tag="KR250_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HBH0068480.1"
                     /translation="MKIRPLADRVVIKKVEAEEKTASGIVLPGAAKEQPQIAEVVEVG
                     PGGIVEGKEIKMELTVGDKVIFQKYSGTEVKIEGQEYTILRQSDVLAVIE"
     gene            12846..14474
                     /gene="groL"
                     /locus_tag="KR250_002552"
     CDS             12846..14474
                     /gene="groL"
                     /locus_tag="KR250_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729227.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HBH0068481.1"
                     /translation="MAKEIKFSEETRRALEAGVNKLADTVKVTLGPKGRNVILDKKFG
                     SPLITNDGVTIAKEIELEDRFENMGAQLVKEVATKTNDVAGDGTTTATVLAQAIIREG
                     LKNVTAGANPILLRKGIQKAVTVAVEELKNQSRIVETQEAISQVASISAGDEEVGKLI
                     AEAMEIVGKDGVITVEESQTMNTELDAVEGMQFDRGFVSAYMVTDVDKMEAVLNDPYI
                     LITDKKISNIQELLPVLEQIVQQGKKLLIIAEDVEGEALSTLVVNKLRGTFDVVAVKA
                     PGFGDRRKEMLQDIAILTGAQVISEELGYDLKEADLSMLGRASSVKVTKESTTIVDGS
                     GDKKAIEDRVTQIKHQVEQTTSDFDREKLMERLAKLAGGVAVVKVGAATEVELKERKL
                     RIEDALNATRAAVEEGIVAGGGTAFVSVIPAIGTLIESLEGEVKLGAQIVKKALEEPL
                     RQIAINAGLEGAVIVQNVVNSEAETGFDALNEKYVNMIEAGIVDPTKVSRSALQNAAS
                     IASTFLTTEAAVADLPEKEDVGMPGMGGGMPGMM"
     gene            14736..15476
                     /gene="larB"
                     /locus_tag="KR250_002553"
     CDS             14736..15476
                     /gene="larB"
                     /locus_tag="KR250_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HBH0068482.1"
                     /translation="MDLRKLLEEVKNENIDIDFALEKLKDLPYEDLGYANIDHHRELR
                     NGYPEVIYCEGKSDEHILGIIDKMNQKSSNILGTRCRKETFLKIKEIYNHAEYEEASK
                     ILKIQNHDIENIGKGKIVIATGGTSDIPVADEAYHTAKFLGNDVDRIYDVGVAGIHRL
                     LNKRHKIDSARVIVAVAGMEGALASVIGGLVDVPVIAVPTSVGYGANFGGLAALLAML
                     NSCASGISVVNIDNGFGAGYLASMINKL"
     gene            15560..16294
                     /gene="larC"
                     /locus_tag="KR250_002554"
     CDS             15560..16294
                     /gene="larC"
                     /locus_tag="KR250_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HBH0068483.1"
                     /translation="MSEKILYFDIISGISGDMTLASLLNLGVPKEIFLEEIHKLRMSD
                     EFNINISSKTENGIVGTKVEVITKEKHTHRNLVDIFEVIDKSTLNENVKSKAKKIFMV
                     IGEAEAKVHGTTIDKIHFHEVGAIDSIVDIVGASILVDLLCVDKVCASTVSVGSGFVK
                     CEHGIIPIPAPATIEILKGVPIKLNNVNGECTTPTGAAIIKVLCDEFVDELEFSPKQI
                     GYGIGHKKFEVPNILRTVLGEKKKKN"
     gene            16324..16743
                     /locus_tag="KR250_002555"
     CDS             16324..16743
                     /locus_tag="KR250_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF111 family protein"
                     /protein_id="HBH0068484.1"
                     /translation="MSSEIYSYLYEKILDEKALDIYTESIYMKKNRPATKVSILCNES
                     DLEKFIKILLLETSTFGVRYNAYNREILERKFVKLDTKYGVVTVKLGYYKGKLVKATP
                     EYNECKTIAKNEDIPIKKVFADINFRIKEEFSENLLT"
     regulatory      16803..16904
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            17125..18660
                     /gene="guaA"
                     /locus_tag="KR250_002556"
     CDS             17125..18660
                     /gene="guaA"
                     /locus_tag="KR250_002556"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HBH0068485.1"
                     /translation="MKHELVLVIDFGGQYNQLIARRVRENNVYCEILPCTASIERIKE
                     KNPKGIIFTGGPNSAYLEDSPTISKEIFELGVPILGICYGIQIMSHVLGGVVRKGNKQ
                     EKEYGKTAITYGKSSLFEGITTNSVWMSHTDLIEKVPEGFTIVANTNDCPVAAMENVE
                     RNLYGVQFHPEVEHCLEGDKILTNFLYNICKVKGDWTTDSFIEDKIKELKEKIGDKKA
                     LCALSGGVDSSVAAVLIHKAIGDNLTCIFVDHGLLRKNEGNDVERIFREKFDINLIRV
                     NAEDRFLSKLKGVSEPEAKRKIIGEEFIRVFEEESNKLGKMDFLVQGTIYPDVIESGH
                     GNAATIKSHHNVGGIPEDVDFQEIVEPLRELFKDEVRKIGLELGIEEGLIFRHPFPGP
                     GLGIRVIGDVTKEKCDILREADAVYMDELRKAGLYREIWQAFATLPDVKTVGVMGDER
                     TYAYLVGLRAVTSSDGMTSDWYKMPYDVLERISNRIINEVDGVNRVVYDITSKPPGTI
                     EWE"
     gene            complement(18737..19783)
                     /locus_tag="KR250_002557"
     CDS             complement(18737..19783)
                     /locus_tag="KR250_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBH0068486.1"
                     /translation="MPAYKDEQRKSWYASFYFTDFDGDRKKKIKRGFKTKKEALEFER
                     EFLNKSKMSTDMSFESLIEEYMHDMSSRLKLSTLETKKYLINFRILPFFKSLKINEIT
                     ATHIRRWQNELLKSDYSQTYIKTINNQLVAVLNYAVKYYNLPSNPAHLAGSIGKKNAD
                     EMNFWTLEEFKKFIEFEKKSEPRLAFEILFWTGLRLGELLALTPKDIFENKISVEKSY
                     IRLNGEDIVSSPKTPKSKRVVPIPHFLYNNIKDYLSKLYDLKDNERIFKFAKSYLSKE
                     LDRCCKLSNVKRIRVHDLRHSHASLLVNMDVNILTIAERLGHEKVETTWNTYSHLYPN
                     KQLEVAQKLDNLNI"
     gene            complement(19860..20075)
                     /locus_tag="KR250_002558"
     CDS             complement(19860..20075)
                     /locus_tag="KR250_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH0068487.1"
                     /translation="MLKKLRKKKRMTQLELAEKMRRNRSYISKLENQEYKDIGISTIL
                     DLSIALEEDFLELCKYYKLQEIKRRGK"
     gene            complement(20509..20910)
                     /locus_tag="KR250_002559"
     CDS             complement(20509..20910)
                     /locus_tag="KR250_002559"
                     /inference="COORDINATES: protein motif:HMM:NF013540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0068488.1"
                     /translation="MQTLAEIIKEYRSEHNLSLREFSNLCGVSHTYIDKIEKNRDPRN
                     GKAVEPTLDMLEKMSFGLNLTLKELLTKLGKIQPNTQQDDELLKKVNSKEDNEVNELI
                     NRLASLDSDDKNAIKKMIDNAYYKAVNTKKE"
     gene            21075..21287
                     /locus_tag="KR250_002560"
     CDS             21075..21287
                     /locus_tag="KR250_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019161272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0068489.1"
                     /translation="MKNNLQYIRKESRISQKKFADKIGISRPYLSRIENGKVNPSLEI
                     AHKISLETGKTINEIFFDFTVNHSKL"
     gene            21527..21664
                     /locus_tag="KR250_002561"
     CDS             21527..21664
                     /locus_tag="KR250_002561"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0068490.1"
                     /translation="MQREQTTLRIPEDLHKALIDLSSDIGMPITSIIIIACWLYISKI
                     N"
     gene            complement(21650..21961)
                     /locus_tag="KR250_002562"
     CDS             complement(21650..21961)
                     /locus_tag="KR250_002562"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0068491.1"
                     /translation="MTTITVRIYTPLNENLEKISYQTGILKSSLILYAINDIIRNSKV
                     NELQSISYKSDDTVRSTLRIPGVLKELLEKTAKENNLSINSLINNVVHSFCISDWLIY
                     L"
     gene            22082..22252
                     /locus_tag="KR250_002563"
     CDS             22082..22252
                     /locus_tag="KR250_002563"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0068492.1"
                     /translation="MEKVRMTVRLVPKLNQYVQNIAKECGKSKNSIIVDACWEFIEKI
                     KKENKFNVESEE"
     gene            22255..22893
                     /locus_tag="KR250_002564"
     CDS             22255..22893
                     /locus_tag="KR250_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0068493.1"
                     /translation="MNNLQIFEKLEFGQIRMAEIDNKPYFVGKDVAKSLGYKNTNDAI
                     LRHCKGVVKHEGFKINGIKIALITEGDVYRLIVGSNLPNAEKFESWVFDEVLPSIRET
                     GQYQAQQHVITELTGTIGDLKGTIKEYKKLCKITCSKKQQYSKYIKNRLDIDKANKEY
                     NQVKARLFLILGVEKWEDIDFDTSNNLIQIIDDCIRVIKSERPYKQLSYFEN"
     gene            22952..23122
                     /locus_tag="KR250_002565"
     CDS             22952..23122
                     /locus_tag="KR250_002565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0068494.1"
                     /translation="MKNNISDSRVKYFCKCPYCGFDNEVEVKKGLKPKICCICTKEVE
                     YEKLEQQSTPAK"
     gene            complement(23125..23358)
                     /locus_tag="KR250_002566"
     CDS             complement(23125..23358)
                     /locus_tag="KR250_002566"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0068495.1"
                     /translation="MNIGKITKIEATCFDCGTKVTITESKFREVCDNGLPCPMCKEHI
                     SNSNFIAEQALQYNHAVNKLEKELDSCKNIHIY"
     gene            23427..23624
                     /locus_tag="KR250_002567"
     CDS             23427..23624
                     /locus_tag="KR250_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0068496.1"
                     /translation="MAINDNINKILRDRNLKAWKLAKEIGVDSGNLYAILRGENKNPT
                     IDTLIKLADYLDVTLDELVGR"
     gene            23663..23872
                     /locus_tag="KR250_002568"
     CDS             23663..23872
                     /locus_tag="KR250_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0068497.1"
                     /translation="MAKAVAKEQLFYRAKDIAKILDVCEATAYKIIGELNKELEKEGF
                     KTFSGRVSVAYFKERYCYKPRKGVI"
     gene            24030..24242
                     /locus_tag="KR250_002569"
     CDS             24030..24242
                     /locus_tag="KR250_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0068498.1"
                     /translation="MVFNLEKFKVGNAIRISCERFGFEIDCIVVVATEKELNLAYFDE
                     GRGCMEYQALITEDIQDGDYEIKILS"
     gene            24341..24583
                     /locus_tag="KR250_002570"
     CDS             24341..24583
                     /locus_tag="KR250_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0068499.1"
                     /translation="MEAARLIAMGQIKQAEKEISKLQGTKNNSSLMWWEAVKFASQNI
                     LQGLEHDIELEASTDFREFMITQEELERDRPIDVQI"
     gene            24667..25176
                     /locus_tag="KR250_002571"
     CDS             24667..25176
                     /locus_tag="KR250_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siphovirus Gp157 family protein"
                     /protein_id="HBH0068500.1"
                     /translation="MSTLYELTTDLLEIEEGLTETTGNEAEKLEEIKEIIKQEIQNKN
                     TRIVSVILNIDSDINSIDSEIKRLQELKRVKKNTLDRLKSNIKDCMELLGTKKVETVL
                     GNISIRKSAGSLVIEDEEKIPAIYKTVEQVVKVDKNTIKDFIKKGHEVEGCRIEYGTT
                     LTIPKAKKE"
     gene            25186..25800
                     /locus_tag="KR250_002572"
     CDS             25186..25800
                     /locus_tag="KR250_002572"
                     /inference="COORDINATES: protein motif:HMM:NF016302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ERF family protein"
                     /protein_id="HBH0068501.1"
                     /translation="MEINNIYIKLMDVRVKFSKLNLKKSGENKFANFKYFELADFLPQ
                     ATGLLEEAKLCPIVTFTNEYATLTLINGENPSEQIVFTSPMRDLQLKGSNELQALGGI
                     ETYQTRYLYIQLLNITENDSFDAVSGKEESKSNSNNNVKQSNRILTDKQLTRLYAIAN
                     SVGVNKALLKEQVFKQLGKDIKDLNKSEYDAVCAAYEKQAFKGA"
     gene            26577..26675
                     /locus_tag="KR250_002573"
                     /pseudo
     CDS             26577..26675
                     /locus_tag="KR250_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420554.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
     gene            26745..26996
                     /locus_tag="KR250_002574"
     CDS             26745..26996
                     /locus_tag="KR250_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0068502.1"
                     /translation="MIIIRSQDRLDLMRVNRVEIGFNQVYAILENDIRKIGEYESIER
                     AMQVLNDIQKFIENGVRTDYIDSCRVRHNQEKVFEMPFE"
     gene            27014..27370
                     /locus_tag="KR250_002575"
     CDS             27014..27370
                     /locus_tag="KR250_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0068503.1"
                     /translation="MAKVWVDAGTFLERTKDIEDMFEFNLRKVRDRNKKINVIDLRVN
                     NEGQGRKGKKVECFNIVTGETKVFNTAVEASKYVYFTDVYIAHLARTGKVSKNGWKAR
                     YIQEVTDGIGKCGTSN"
     gene            27339..27488
                     /locus_tag="KR250_002576"
     CDS             27339..27488
                     /locus_tag="KR250_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0068504.1"
                     /translation="MVLANVEQVIKLAEKILNKKKCSVNKAIDIVIKILSKYECEGML
                     RNEFN"
     gene            27475..27924
                     /locus_tag="KR250_002577"
     CDS             27475..27924
                     /locus_tag="KR250_002577"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0068505.1"
                     /translation="MSLIKYRGYDFENEKWIYSATIMWSNILECLVMLTEGCKWQKVS
                     NVGVCSGEWARNNQEICEGDILKGYDNFHDTSQYGVVKRDIGSIKLYLEWHYLKKLEG
                     EWIELINKTEIYHSRDYKIVGNEYENLEEVRTEFLERKESLENEYLS"
     gene            28027..28203
                     /locus_tag="KR250_002578"
     CDS             28027..28203
                     /locus_tag="KR250_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0068506.1"
                     /translation="MDDRLEMINASVNYIQMICESSNIAIIAERGRVRILDLETKEKY
                     DLLKNKLEEMLEEI"
BASE COUNT        11290 a         2893 c         5149 g         8947 t
ORIGIN      
        1 tttagaggga tttaataatg tttcaactat aagtaataat attatattgt acacttggat
       61 tataactgta gtagtgtatt taatttacac tatatttaaa tatataaaac ttaaaaattt
      121 aataaatgac ttatcttatg atgttgatga tagagagatt gtaaatcttt ataaaaatat
      181 attagaagaa tttaatataa caaaagatat aaaactcaaa tactcttatg aagttgaaac
      241 accagcattt tttaattcat gtgtactttt gccaccacat gagtataaat tgaaagagtt
      301 agactggatt tttagacatg agcttatgca ttttaagagt agggaccttt atttaaaata
      361 tgtaatccta tttttaaaaa cagtatattg gtttaatcca tttatatata taatggataa
      421 acatatagat ttagattgtg aactttattg tgatgaaaga gttttaaaaa atcgaaattc
      481 agaggaaaaa aaggattatg ctttaacaat tctaaatgca atgagaaaag gttcaaatac
      541 ttcaaataaa tttatagctg gacttcataa gcaatctgat attaaaaaga gagttatgaa
      601 tatgtttaat gaaaagtaca agaatggtat attaatggca ttaactttat gtttattatc
      661 ttctattact tttttgaagg ttaattcaac tagtattgtt aatcttaatg tattacctaa
      721 aggatttgaa gacttacgaa ctgttagcag tatacgtttt tcagttatag gatttacata
      781 tgcagatgct cctaaaaata tgagggaaga atataaaata aattgtgaat cattaggttt
      841 gatacctaaa gactctgata aaatagaagt tggtatagaa tattatgaca gtctattgaa
      901 tgattaaata aatattattt taacagatat taattataaa gttttgaata gtaatttgtt
      961 gagaaataaa tatatcctta taaaaataga tacttatata ccaagaataa aaaatagtca
     1021 agatagtaca caaggatata cttataaaga acttaaataa gtgaaataaa ttattaatta
     1081 taaggtttat aattttaaaa aattagataa attcaggtgt aaaatactat aaaacacaat
     1141 tcggaaaagt gtatgttgaa cttcatttaa aatggaattt caaagttgct atagtaagta
     1201 gtttaagcat tcttcttata ttaatctttg agaataatgt atttaaaaaa gttagtaaaa
     1261 aatttaacta ttgtatatta atgatagttg ctattgatat attatctaaa aaaattaagt
     1321 atgaaaagat tactgctagg acaatctttt catacttaat ttttaatatt tatattatcc
     1381 aaaattttca tctttttaac ttagaatttg agaatcacta ttaggaaata ggctttgatt
     1441 tatgtagggt taaaaatttc acagaatatc taactttata ctaaatccat caaaaaatat
     1501 acgccataca aaagcgcata aaaaaacaca taaaacaata tttatgcgca ctttaacctc
     1561 gaatggtaca aaattcttta cttttacaga cccatattat ataattaagt aaataatgta
     1621 tatttaggtg acatattgga taagctaatt tatttatttt attaatataa taatactata
     1681 aaagttatat aaagtggttt acaaaaaagt tacagataga gcttttatta atgtattgtg
     1741 tattagtatt ttatccttag gtacacttca cctacaaaat agaatcataa gataattatg
     1801 ggaaaatttt aggaggttta tcgtgaagaa aaagatatta ataccagtat ttgcatcagt
     1861 tatggcatta tcagtaagtt caattgtaaa tgcagatgaa gtaaatgatt catctcaaaa
     1921 taaggatgat aaaacaaata ctgaattaaa tttaggtgaa tataaagaag taaaatataa
     1981 agttgcaaaa ataaaagatg gagtagctat aaagataaga gaagaaggtc aagttcaaaa
     2041 catagcttat tctggagatg aatttacggt tttaggaact caaggagaat gggttaaggt
     2101 taaagttgaa gatggcgaag gttggttagc tactagatat gtagatatat ctgaaggtgt
     2161 aggatatact aacgctgata aagtaaactt aagaaaagat aagagtgaaa gttcagaagt
     2221 aattgaagag ctggaaaaag gaagttcttt attggttttg gaagataatg gagattggtt
     2281 aaaagttaaa gatggggaaa cagaaggata tgtaaaatca tcttatatat ctgataaagc
     2341 tcctgtaata gaagagccac aagtaaaccc aagtattgac caaaatgtag attctacaaa
     2401 taataatcaa caaaacaatg caaatactaa tcaacaaaat aataattcaa atgtgccaac
     2461 agctaactct aatgcagttc aagctgtact taacttggca tattctaagc aaggttgtcc
     2521 atatgtttgg ggagcagaag gaccaaacac ttttgactgt tctggattta cacaatatgt
     2581 gtatagaaat gcagtaggaa agaatatacc aagaacatct aaagcacaat ctaaatatgg
     2641 acaaactgta agtaaagcaa atttacaacc aggagattta gtattcttca caactaatgg
     2701 ttctggttct gttagtcatg ttggaatata tgtaggtggg ggtaatatga tacactcacc
     2761 atcaacagga aaaacagtta gtgtaactag tataaattca tcatactata cagcaagatt
     2821 tgttacagca aaaagaatat tataatcggt ttgtaatatg acaaattgat aaatacataa
     2881 atatatataa acgctctata ttatatttaa tttaatcatg gaaacaaaat aatgattata
     2941 ttttaaatgt actatagagc gtttttgtat cactttaaat ctaatcaaga ttcaagttaa
     3001 tatgactgtt aaataggagc cttttcatat gatgattatt tgtgcaatcc cttgacagga
     3061 tagggtaaag gtggtaagct actaacagta caaaaatact tttaatttta gttcagagag
     3121 gctaaaaaag gaggacgaaa gatgttaaaa tcaagaaatt taatccaacc agaagacttt
     3181 tcaatagaag aaatagatga aatattagaa ttagctcaaa agataattga caatccgtca
     3241 aaatattctc gtatatgtga aggaaaacta ttagctacat tattttatga accatcaaca
     3301 agaacaagat taagctttga atctgctatg aacagattag gaggacgtgt agtaggcttt
     3361 tcagagccaa attcatcctc tgcatcaaaa ggtgaaactc taggagatac aatgaggata
     3421 gtatctggtt atgttgatat aatagctatg agacatcctc aatcaggtgc agcttcagaa
     3481 gcagctagat atacggaagt gccatttata aatgcaggag atggcaaaaa ccagcatcca
     3541 actcaaacac ttacagactt acttactata aaatcattaa aaggaacttt agaaagccat
     3601 acaataggat tatgtggtga tttaaaatat ggaagaactg tgcattcttt ggttaaggct
     3661 atggcaagat ataaaaatac aaagtttgta tttatagcac ctgaagagtt aaaaatgcca
     3721 gattatataa aagaagctat aaagggacat gcatattatg aaactaataa tttggatgat
     3781 gttataggaa gcttggatgt tttatatatg actagagttc aacaagaaag atttgaagat
     3841 aaatcagagt atgaaagact taaaaactat tatattttaa ataaagctaa attagaaaag
     3901 gcgtctaaag atatgttagt aatgcatcca ttaccaagag taaatgagat agacatagat
     3961 gtagatagtg atgatagagc tgtatatttt aaacaagcta aatatggaat gtatgttaga
     4021 atggctttaa taataaaact tttaggtata aatgaagact aatagttatc atataatagt
     4081 aaaacaaagg agattttggg atgtataaaa ttctggaaaa tatatatata ggcgaagata
     4141 tgtatagaat gaaagtaaaa ggtaattttg aaggtaaaat gggacaattc tatatgttaa
     4201 gagcatggga tacttatcca gtactttcaa gacctataag tatacatgat atagatgaag
     4261 aagggataac ttttctttat aaagtagtag gagaaggaac tcaaattttg agtaacctaa
     4321 aggtaaatga tactataaaa ttggaaggcc cttatggtaa tggatatgct aaggttgatg
     4381 gaaaggtagc tctagtagga ggaggaatcg gtgtagctcc actatatttg gttgctaaaa
     4441 atataaaaaa ttgtgatgca tatcttggct ttagagaaga tgttatatta gaagatgaat
     4501 ataaacaagt atgtaataaa gtatatacaa cagtaggaaa tacttttgta acagatataa
     4561 ttgatgttga gaaatatgat tatatattaa catgtggtcc aactccaatg atggaaaaat
     4621 tagttaagat ggtagaaggt actaaaacga gaattatggt atctcttgaa aaccatatgg
     4681 cttgtggagt aggtgcctgt ttagtatgta cgtgtaaaac taatggagga aataaaaaga
     4741 cttgtaaaga tggtccagtg ttctggggag aggacgtgat ttttaatggg taatttaagt
     4801 gtgaaatttg gaagtgtaga atttaaaaat ccagtaataa tggcgtctgg aacttttgga
     4861 tttggaaaag aatataatga gatttatgat atacaaaaac ttggtggtat aagtagcaag
     4921 ggtcttactt taaataaaaa acctggtaac aatggtatga gagttcatga gacaccatcc
     4981 ggtatgatga atagtgttgg gcttgaaaac cctggagttc aaggatttat agactatgaa
     5041 ttaccttttt ttagtaaact agacttagtc agaatagcta atgttggagg tgggacatta
     5101 gaagactact tattaggggt tcaaatgtta aatgacaagc ctatagacat tattgaatta
     5161 aatatatctt gcccaaatgt caaagctggt ggaatggctt ttggtataaa aaatgaagtg
     5221 gcaagagaag ttgttcgtga ggtaagaaac ataacgaaac tgcctttagt aataaaatta
     5281 tcacccaatg cagaagatat tgtaggaatg gcaaaagttt gtgaagaaga aggtgctgat
     5341 ggagtttcat tagttaatac ttttaaagca atggcaattg atataaaaaa tagaagacca
     5401 gtatttgaaa atgtatatgc tgggttatct ggaccagcta taaaacctat agctttgaga
     5461 atggtacatg aagtgtgtaa aaatgtaaat atacctgtaa tgggaatggg cggaataact
     5521 aaagccacag atgctataga gtttataatg gcaggagcta cttgtatcca agttggaact
     5581 gcaaatttta tgaatcctag aataggtatt gaaataatag atggaattaa tgaatttatg
     5641 gatagagaag gaataaaaag tttagacgaa ataagaggaa ttatataagg tagaataaat
     5701 agtattttaa agataaaaaa tatagattaa ataaacaata attttataac attaacatgg
     5761 aggaaaaata atatgagtaa agtagatgta gtggatatat taaagaaaag tgatgcattg
     5821 ttggagggac attttttatt atcttcagga aaacacagta atagatacgt acaatgtgca
     5881 aaagtattaa gatttccaga atatgctgct gaggtattaa gtacagttgt tgaacaaata
     5941 aaagacttag atatagactt agtagtagga ccagctatgg gtggagtaat agtttcttat
     6001 gagttaggaa gacaattagg aaaagaagct gtatttactg agagaaaaga caatacaatg
     6061 gagttaagaa gaggatttga agttaaaaaa ggagcaaaga taataattgc tgaagacgtt
     6121 gtaactactg gtaaatcaac tatggagaca aaaagagtat tagaagcctt aggtggagaa
     6181 gttgtaggtg ttgcatgtat agcagataga actaatcatg atataggtat gcctatatat
     6241 agtgctataa aacttgatat tcaagtttat gaatctgatg agtgtccttt atgtaaggaa
     6301 ggaaaattac cagttgttaa gcctggaagt agggagttca aggaattagg gatgtaataa
     6361 aaacttaatt atttatagtg ttacttaaaa aatgtaaatt ttttagagta aaaatttgta
     6421 atataacata taacttaaaa ataaaaagca gttagcctta taaatgaagg ttagctgttt
     6481 tttttgttaa aaatatattt acaaaataag agatagtagc agtacaatat aagtataagg
     6541 taaaagtatt atatattagg gaggggtaag tatgaaaaat caattaaatg aaaatcataa
     6601 aaaggttgta ttttttggag taggtgctgt aggagctact tttgcagaac aattttttaa
     6661 ctctaaatat gattttaaaa ttctttgtga taatgaaaga aagaaaagat atttagaaga
     6721 aggatttata ataaatggaa aaaggtatga ttttgattat gtaactaaag atgagtataa
     6781 acaagaggct gattttataa ttataggtct aaaatataat aatttaaaag aaaatataaa
     6841 agaattagat ggattagttg gaaaaaatac agttataatg tctctgctaa atggagttga
     6901 tagcgaagag ataataggag aaagatttgg aattgaaaaa atggtatatt catatgttac
     6961 caatatagat gcaaagaaaa tcaataataa tattatacac actactaatg ggataattgt
     7021 atttggtaac aaagataata gtgaagatag aaaaactaat ataataaccg aagtctttga
     7081 tgatgtaaat atagaatata ctttatcaaa agatattcaa cgagatatgt ggtggaagta
     7141 catggttaat attggtgtaa atcaaacttc agctatactt ggtgcacctt atggagtttt
     7201 tcagagttct gagcatttaa gagaattagc aaaatctgca atgagggaag ttgttgctat
     7261 agcacaagca aaagacatat ctcttacaga agatgatgtg gaacattcat tacatagaat
     7321 actagaacat tcaaaagaag gaagaacatc aatgcttcaa gatgtggaag ctcatagact
     7381 tacagaagta gatatgtttt ctaagaatat ctgcaaactt ggaaaaaaat ataacatacc
     7441 tactcctata aatcagactt tcttttatat gataaaagta attgaaagta gattttaaag
     7501 ggtctttgta agaaatattg ataattattg attttaaatt ttattaatgt tatatactat
     7561 ttaagttact aaatattttt aactcttcaa tgaaaatagt tgctgctttt gataaagtta
     7621 tatcttttgg aaatatatat gagaaatgtc tgttgtagct gcttccaagc tctatgacag
     7681 acagctcatt gtttagaaca ggtaagcttg tcactagatt agagactatt gtaattccta
     7741 aattatttct aacagattct ttgacagcat aattacttcc aaaaaccatc atactttttg
     7801 gaataatttc tttaacactt aaaaacatat ctaaataatc tctggttcca gaaccatcct
     7861 ccctgactat ccatttttgg ttttgaagtt tatcaaaact aaaatctttt aataaatgac
     7921 tcttgtatgg aagtgctaaa accatcttat cttcaaaaaa atattcttga ataaatgaag
     7981 gggaagagca agtgccttca ataagaccaa tgtctaaaat ataatcctta acatgagaag
     8041 atacgattga tgtatttttt ataaaaactt ctacatctat gtcaggatac tttttagaaa
     8101 ataatgctag aaaatttggt aaaatatact ctccaatagt taaacttgca ccaatcttta
     8161 agtgaccagt tatagcatct gaaacttggc ttacatcatg ataagtaata tttaaaagat
     8221 ttaatatttc tttagctctt ttgtacaaga tttgtccact ttctgttata aaaatggttt
     8281 tttgtttaat agacctgttt ataagcacaa ctttaaaata attttctaga ttttttatgt
     8341 gtttacttac acttggttgt gatagattta aatattcccc agctttagta aagtttttgt
     8401 attcaacaac agctacaaat gtttttaatt cttcaaacaa ttttttgtct cctttaccta
     8461 atgaaatttt acatttttta tatactttct acagataatt atactatata taataaccat
     8521 taaaaacatt aatcattacg atattatata tttattttac ttatggttat tacaattata
     8581 tactattgct atgaagaaaa ctatattaat tataaggtag gtatattatg ttaaagaaaa
     8641 aaagcagttt gacattaaaa actataaggg aaatattacc aggactgttt gtatcagtat
     8701 tagtaggtta tattagtata tttatttcta ttttaattcc taaggtaggg gcagcatcaa
     8761 tttctatatt tttaggtatg tttgtaggaa acctattttt aaatcaaaag gtatttcaaa
     8821 aaggttataa attttcagag acagatttgt tatcatattc aatagtacta ttaggagcaa
     8881 cactcagtgt atctacctta atagacttaa aagtttcagg aattttattt ataatattgc
     8941 aaatgacaat aacaataata gctgcacttt atataggtaa aaaactagga tttgaagaaa
     9001 actttagatt tctaatggca agtggaaatg cagtatgtgg ttcttctgca ataggagcaa
     9061 cagttccagt ggttaatgcg acagataaag aaaagggaat tgcagtaact attgtaaatg
     9121 ttactggtat atttcttatg tttttacttc caataatatc tcaattttta tatagccatg
     9181 aacttgtaaa aacttcagca atgataggtg gaactttgca atcaattggt caggttgtag
     9241 caagtggagc aattgttgga gaaaatgtaa aagatttagc tactattttt aagatagtaa
     9301 gagtaatatt tctagttgtt gttgtattag tatttggaca tatgaaaaat caatctaata
     9361 gggaaattct tgaagaagaa aaggatgaga taaaaaagaa aaaagtgact ataccatggt
     9421 atgtaattgg attctttata acttgtgcac ttttttcaat gaacattata ccacaagaag
     9481 tatctgtgtt gtgcaaagaa ataagtaaca aactagaaat aatagcttta gcagcaattg
     9541 gattaaaggt taatgtaaaa gatttagtga aacaagggaa agaagtttct ttatatggtt
     9601 tatttgtagg tatagtacaa gttgtatcag ctgtagtttt aataaaaata tttatatagc
     9661 ataaaaatag tatttgtaat attaatttga ttaattaaaa taatatgata ctaataattt
     9721 ataatataaa taaaaaatga atttcgataa gtttaagtgg gtataaactt atagaaattc
     9781 attttttatt taaggtaaaa atttaaagta aaatattatt ttacataata ataaccttat
     9841 aaattttgaa attacttatc tttgtgttaa aataagtaaa atgcacatat tagagctaag
     9901 tttttaagta aataaacaaa atgaaaatat aaatattaag gagatttggt atatgaatgt
     9961 ttatgaaaaa ggtaatggag ttatactaga aggagtaaca gattttgacc cagtacatat
    10021 atttgagtgt ggtcaatgtt ttagatggca caaacaagaa gatggctcat acactggagt
    10081 tgcaaagggg agaatattaa atgtcaaaaa agagaatgat aaaatatatt taaataatac
    10141 taatttaaag gagtttaaca gcatttggta taattacttt gatttaggaa ctgattatac
    10201 agaaataaag aataagctaa aaaatatgga tgaatattta aataaggcta ctgaatttgg
    10261 atggggaata agaatattaa gacaagatgg atgggaaatg cttatatcat ttataatttc
    10321 ctctaataat aggattccta tgatacaaag agctatagaa aatttatcaa gaaaatttgg
    10381 taagtatata ggtgaatatg aaggtaatga atactatgca ttcccaacac cagaagaact
    10441 aaataaggcc tcacaagagg aaataagagc atgtcaaaca ggttttagag ataagtacat
    10501 aaaaagtact acacaggcag ttatagagaa taatgatgag gtatctgaat atacaaattt
    10561 aagtactgag gattgtagaa aagagctttt aaaatttaat ggagtaggac caaaagtatg
    10621 tgattgtatt gcattatttg gtatgcaaaa atatgattct tttccagttg atgtttgggt
    10681 taagagagtt atgcaagaat tttatataga tgaagacatg agtttaccta aaatgagaac
    10741 ttatggaatc gataaattta aagaaatgtc tggatttgct caacagtact tattttacta
    10801 tgcaagagaa ctcggtatag gaaaataaat taagggggag aaattatggg agtaatagga
    10861 acaatatttc tgttctattt aattatatca tatctagctg gagccataat ttctgtgatt
    10921 atattgcttg aaaatagaga ccctgcaaaa acaatgtctt ggctattgat gtttattata
    10981 tttccaggtg taggtttaat gatatatgct atttcaggta gaaatattag aaaaagaaaa
    11041 ttatttaaga ctcaaaaact agctaataat ataaaagaaa agaaactatt tgatacatta
    11101 gaaaagataa cagaaatagt tgaattagaa aaagaatcta taaaacaaaa taagctttta
    11161 agagatgaag aggatggtag ttatagaaaa agggtaataa atatgttgtt aaagactggt
    11221 atgtttccct ttactaaaaa taataaagtg gatgtatttg tagatggaaa tgaaaagttt
    11281 aaaagactta tagaagatat aagagaagcc aaagaccata ttcatttaga gtactttatc
    11341 atcaaagata gtgaaatagg tagggtgtta aaagaagaac ttataaaaaa ggctaaagaa
    11401 ggcattaaga taagaatttt atatgatgat gtaggatgct ggagattttg gtttaataga
    11461 aaatttttcc gagaaatgag ggaagtaggt atagaaattg cagcattttt acctacaaaa
    11521 tttcctataa taggagggaa attaaactat agaaatcaca gaaaaattgt tgttattgat
    11581 ggaataattg gatatacagg tggaataaat ataggagatg aatatttagg caaaaatgat
    11641 aagtttggat attggagaga tactcatatt aggattaagg gtatttctgt atacatgctt
    11701 caaatgacat ttctgattga ttggtattat acgacaaaag aagtattagt gactaaaaat
    11761 tatttcccaa gtgtagggaa tgttggtgaa agtatgatac aagttgttgc gagtggtcca
    11821 gacagtgact gggaagatat tcattatgct tatttttctg caatatgtca ggctagaaaa
    11881 aatgtttata tagaaactcc atattttatt ccagatgaaa gtctactaaa agctataaag
    11941 agtgctgctc ttagtggagt tgatgtgaga ataatatttc ctaaaattgc agaccacaag
    12001 atagtaaata tagcatctta ttcatatttt gaagaaatat taagagcagg tggtaaagtt
    12061 tatttatata ataaaggatt tatacattct aaagtcgtaa taatagatga taaaattgct
    12121 tctgcaggta cagctaatat ggatttaaga agttttatgc ttaactttga ggtaaatgca
    12181 tttatctatg atgaagaagt tattagggta atgacagatg atttctttga ggatttaagt
    12241 cactgtgaag aacttaattt ggaggtattt aagaatagaa atatcataca aaaaatcaaa
    12301 gaatctgtag caagattatt ttcaccaata ttataaaaaa ttattaaaaa aggtattgaa
    12361 aattatgtgt gattggtata ttataataat tgttagcact ctattagata gagtgataat
    12421 aaataaaaag tatatatata aatctacata tataagtagt attaatttaa aaataactaa
    12481 aaacataggc gaactagtct atacttaata ttttaggagg cgtgaataat gaaaataaga
    12541 ccattagctg acagagtagt aattaaaaaa gtagaagcag aagaaaaaac tgcaagtgga
    12601 atagttttac caggagcagc taaagagcaa cctcaaatag ctgaagttgt agaagttgga
    12661 ccaggtggaa tagttgaagg gaaagaaata aaaatggaat tgacagtggg agataaggtt
    12721 atattccaaa aatattctgg aacagaagtt aagatagaag gacaagaata cacaatacta
    12781 agacagagtg atgtattagc tgttattgaa taaatataga ataaatttat taggaggggt
    12841 ttaaaatggc taaagaaatt aaattttcag aggaaacaag aagagcttta gaagctggtg
    12901 taaataaatt agcagataca gtaaaagtaa cattaggacc taaaggaaga aatgttatat
    12961 tagataaaaa atttggttct ccacttataa ctaatgatgg agtaactata gcaaaagaga
    13021 tagaactaga agatagattt gaaaatatgg gtgctcaatt agttaaagaa gtagcaacta
    13081 aaactaatga tgtagctgga gatggtacta caactgctac agttttagca caagctataa
    13141 taagagaagg tttaaagaat gtaacagcag gggctaaccc aatactttta agaaaaggaa
    13201 tacaaaaagc agtaacagta gcagtagaag aattaaaaaa tcaatcaaga atagtagaaa
    13261 cacaagaggc tatatctcaa gttgcttcta tatctgctgg tgatgaagaa gttggaaaat
    13321 taatagcaga agctatggaa atagtaggta aagatggagt tataactgtt gaagagtctc
    13381 aaactatgaa tactgaatta gatgctgttg aaggtatgca gtttgataga ggatttgttt
    13441 ctgcatatat ggttacagat gtagataaaa tggaagcagt tttaaatgac ccatatatat
    13501 taattactga taaaaaaata tctaacatac aagaattatt accagttctt gaacaaatag
    13561 ttcaacaagg taaaaagtta ttgataatag ctgaagatgt agaaggtgaa gcattatcta
    13621 cattagtagt aaataaatta agaggaacat ttgatgtagt agcagttaaa gctccaggct
    13681 ttggagatag aagaaaagaa atgcttcaag atatagcaat acttacaggt gctcaagtaa
    13741 tatcagaaga attaggttat gatttaaaag aagctgattt atctatgcta ggtagagctt
    13801 catctgttaa ggtaactaaa gaaagtacta caatagtaga tggttctgga gataaaaaag
    13861 ctatagaaga tagagtaact caaattaaac atcaagtaga gcaaactact tcagattttg
    13921 atagagagaa attaatggaa agattagcta aacttgctgg aggagtagct gttgtaaaag
    13981 ttggagctgc tacagaagta gagttaaaag aaagaaagtt aagaatagaa gatgctctta
    14041 atgcaactag agcagctgta gaagaaggaa tagtagctgg tggaggaact gcttttgtta
    14101 gtgttatacc agcaatagga acactaattg agagtttaga aggagaagtt aaactaggtg
    14161 ctcaaatagt taaaaaagcg ttagaagagc cattaagaca aatagctata aatgctggtc
    14221 ttgaaggtgc tgtaattgtg caaaatgttg ttaattctga agcagaaact ggatttgacg
    14281 cattgaatga aaaatatgta aatatgatag aagctggtat agttgaccca actaaagtta
    14341 gtagaagtgc tttacaaaat gctgcatcaa tagcaagtac tttcttaact actgaagctg
    14401 ctgttgctga ccttcctgaa aaagaagatg taggaatgcc aggaatgggt ggcggaatgc
    14461 ctggtatgat gtaatttaaa aaatttaaaa tataaatttt aatataaaga actatttcga
    14521 cttagatttt aaactctaag ttgaaatagt tcttttgtat atatctaatt tttgtatatc
    14581 tataaattca atacacatat ttagataatc agtatctagt ttgtaataat attttagagt
    14641 agtattgtta ttttttaggt tatgatttta gatatatatt aattaaggta taattgttga
    14701 tagagaataa atcaaattta aaaggatgcg ataatatgga cttaagaaaa ttgcttgaag
    14761 aagttaaaaa tgaaaatatt gatatagatt ttgcgttaga aaagttaaaa gatttacctt
    14821 atgaagacct tggatatgca aatatagacc atcatagaga attaagaaat gggtatccgg
    14881 aagttattta ttgtgaaggt aagagtgatg agcatatttt agggattatt gataaaatga
    14941 atcaaaaaag ttctaacata ttggggacaa ggtgcagaaa agaaactttt ctgaaaatta
    15001 aagaaatcta taatcatgct gagtacgaag aggcgtctaa aattcttaag attcaaaatc
    15061 atgatataga gaatatagga aaaggtaaaa ttgtgattgc tacaggggga acttctgata
    15121 taccagtagc agacgaagct tatcatacag ctaagttttt aggtaatgat gttgatagaa
    15181 tatatgatgt tggagtggca ggtatacata ggcttttaaa taaaagacat aaaatagata
    15241 gtgctagagt aatagtagcc gttgcaggta tggaaggtgc tcttgcaagt gtaattggtg
    15301 gactggttga tgttccagta atagctgttc caacttctgt tggatatgga gcaaattttg
    15361 gaggattagc agcattacta gctatgttaa atagctgtgc atctggaata tctgttgtaa
    15421 atattgataa tggatttgga gcaggatatt tagcatcaat gataaataaa ctgtaaatac
    15481 attgatataa attaaacaaa actatataat aaaatttaat ttctattata tttttactat
    15541 taaaattgag gagacatata tgagtgaaaa aatactttat tttgatataa taagcggtat
    15601 atcaggggat atgacccttg caagtttatt aaatttaggt gtgcctaagg aaattttttt
    15661 ggaggaaatt cataaactaa gaatgagcga tgaatttaat ataaacatca gctcgaaaac
    15721 tgaaaatgga atagtaggta caaaagtaga agtaattaca aaagaaaaac atacacatag
    15781 aaatttagtg gatatatttg aggttataga taaaagcact ctaaacgaaa atgtaaaatc
    15841 taaagcaaaa aaaatattta tggtaattgg agaagcagaa gcaaaagttc atggaactac
    15901 aatagacaag atacattttc atgaggtagg ggctatagat tcaattgtag atatagtagg
    15961 agcttctata ttagttgact tactttgtgt agataaagtt tgtgctagta cagtgtctgt
    16021 tggttcagga tttgtaaaat gtgaacatgg aattatacct atacctgcac ctgcaactat
    16081 agaaatttta aaaggtgtgc caattaaatt aaataatgta aatggagaat gtacaactcc
    16141 aacaggagct gcaatcataa aagtcttgtg tgatgagttt gtagatgagc ttgaattttc
    16201 tccaaagcaa attggatatg gtataggtca taagaagttt gaagtaccaa atatactcag
    16261 aactgtatta ggtgaaaaaa aaaagaagaa ttaatttatg aaattagtgc aaacattgat
    16321 gatatgtctt cagaaatcta ttcttatttg tatgaaaaaa tattagatga gaaagcatta
    16381 gatatttata cagagagcat ttatatgaag aagaatagac cagcaacaaa agtatcaatt
    16441 ttatgtaatg aatcagattt agaaaaattt attaaaatat tacttttaga aactagtact
    16501 tttggagtga gatataatgc atataataga gaaattctag agagaaaatt tgtaaaatta
    16561 gatacaaaat atggagtggt tacagtaaaa ctagggtatt ataaaggaaa actagtcaag
    16621 gctactccag aatataatga atgtaaaact atagcaaaaa atgaagatat tcctataaaa
    16681 aaagtgtttg ctgatataaa ttttagaata aaagaagaat ttagtgaaaa cttattgaca
    16741 taagtaataa attttgataa attaatattc agccttatta aataaaagta aacagaaaaa
    16801 aatatataaa atataaactc actcgtatat gctcagaaat atggtctgag agtctctacc
    16861 aagataccgt aaatatcttg actatgagtg aaattattat accaaaagac ctagttaagt
    16921 aaatgtgtag gctgagttaa actccctatg tgttggctat aagataaagg tattttggaa
    16981 gcctgtatag taatttcact tttgctagaa attatggaag ttactataca ggttttttgt
    17041 gtaaaaagat ttaatataat ttagactgta ttgtgttaaa ataaatatat aaaaactata
    17101 ttatacaagg agagatgtaa atttatgaaa catgaattag tacttgtaat agattttgga
    17161 ggacaatata atcagttgat agctagaaga gttagagaaa acaacgtata ttgtgaaata
    17221 cttccatgta ctgcaagcat agaaagaata aaagaaaaaa atcctaaagg gataatattt
    17281 acaggcggtc caaatagtgc gtatttagaa gattctccaa caatatcaaa agaaatattt
    17341 gaactaggag taccaatact tggaatatgc tatggtattc aaataatgtc tcatgtattg
    17401 ggtggagttg taagaaaagg taataaacaa gaaaaagaat atggaaaaac agctataact
    17461 tatggaaaat catcattatt tgaaggaata actacaaata gtgtatggat gagtcataca
    17521 gatttaatag aaaaagttcc agaaggattt actatagttg caaatactaa tgattgtcca
    17581 gtagcagcta tggaaaatgt agaacgaaac ctatatggag ttcaattcca tccagaggta
    17641 gaacactgtt tggaagggga caagatactt acaaacttct tatataatat ttgtaaagta
    17701 aaaggtgact ggactacaga ttcattcata gaagataaaa taaaagaatt aaaagaaaaa
    17761 ataggcgata aaaaagcttt atgtgcttta agtggaggag ttgattcttc tgttgctgca
    17821 gttcttattc ataaagcaat tggggacaac ttaacttgta tatttgttga ccatggttta
    17881 cttagaaaaa atgaaggaaa tgatgtagaa agaatattta gagagaaatt tgatataaat
    17941 ttaataagag ttaatgcaga agatagattc ttatctaaat taaaaggagt ttctgaacca
    18001 gaagctaaga gaaaaataat aggtgaagaa tttataagag tatttgaaga agaatcaaat
    18061 aaattaggaa aaatggattt cttagtacaa ggaacaatct atccagatgt tatagaaagt
    18121 ggacatggaa atgctgctac tataaagtct catcataatg ttggtggaat cccagaagat
    18181 gtagacttcc aagaaattgt agaaccatta agagagttat ttaaggatga agttagaaaa
    18241 ataggattag agcttggtat agaagaagga ttaatattta gacatccttt cccaggacca
    18301 ggtctaggaa taagagttat aggtgatgta actaaagaaa aatgtgatat attaagagaa
    18361 gctgatgctg tttatatgga cgagcttaga aaagcaggtt tatacagaga aatatggcaa
    18421 gcttttgcaa cattaccaga tgttaagact gttggagtta tgggtgatga aagaacatat
    18481 gcttatttag ttggtttaag agcagttact tcttctgatg gaatgacttc tgactggtat
    18541 aagatgcctt atgatgtatt agaaagaatt tctaatagaa taataaatga agttgatgga
    18601 gtaaatagag tagtttatga tataacttct aaacctcctg gaactattga atgggaatag
    18661 taaataaaaa agtcgtaagc cttgtatttt caaggtttgc gacttttaaa aaaatctttg
    18721 tgatactaaa atgatactat atatttaaat tatctagttt ttgggcaact tcaagttgct
    18781 tatttgggta taaatgtgaa tatgtattcc aagttgtttc tactttttca tgccccaatc
    18841 tttctgctat agttaatata tttacatcca tatttaccaa taatgacgca tgagaatgtc
    18901 tcaaatcatg cactctaatt ctttttacat tagataactt acagcatcta tctagctctt
    18961 tagaaagata acttttagca aatttaaata ttctttcatt atcttttaag tcatatagct
    19021 tagatagata atcttttata ttattatata agaaatgtgg gatagggaca acacgtttac
    19081 ttttaggagt tttaggagaa gaaacaatat cctctccatt tagtcttatg tagctttttt
    19141 cgacgcttat tttattttcg aaaatatctt ttggagtaag agctaacaac tcccctagtc
    19201 taagacctgt ccaaaataaa atttcaaatg ctagtctagg ttctgatttc ttttcaaatt
    19261 caataaattt tttaaactct tctaatgtcc aaaagttcat ttcatctgcg ttttttttac
    19321 cgatagaacc tgccaaatga gcaggattag aaggtaagtt ataatattta actgcataat
    19381 ttaatacagc aacaagctgg ttgtttatcg ttttaatata tgtttggcta taatcacttt
    19441 ttaataattc gttttgccat cttcttatat gtgttgcagt aatttcattt atttttagac
    19501 ttttaaaaaa aggtaaaatt ctaaagttta ttaaatactt tttagtttct aatgtagata
    19561 attttaatct agaagacata tcgtgcatgt attcttctat gagactttca aaactcatat
    19621 cagtactcat tttagattta ttcaaaaatt ctctttcaaa ctctagagct tcttttttag
    19681 ttttaaaacc tctcttaatt ttctttttcc tatccccatc gaagtctgta aaataaaagc
    19741 tagcatacca actttttctt tgttcgtctt tgtaagcagg cattttagac acccccaatt
    19801 atgtcatttt aatattacaa taattataac atgttagata tgctatctaa ataattgttt
    19861 tattttcctc ttctttttat ttcttgaagc ttgtaatatt tacacagttc taagaagtct
    19921 tcttctagtg ctatagataa gtctaatatc gtagatatac ctatatcttt gtactcttga
    19981 ttttctagtt ttgatatata acttctgtta cgtctcattt tttctgctaa ttctagttgt
    20041 gtcattcttt tcttttttct taattttttc aacatttatt aaaatccacc ttttaattat
    20101 ttttatgaca tttttataat taatagtttg tgtaatttct tgtaaaaaat gttcctcatt
    20161 agaacatttt ttggtggaaa aatgtgttaa aatgtaagta agaaaagcac tttcaaagca
    20221 ctataaaaat agtgctgaaa tagataaaca aataaaagta ataaggagga aagatagatt
    20281 ctagagggaa atcattatca tttttaagaa cgtatgttcg gttggtggga tagaaaaata
    20341 ctttacaggg gatggtttat ttgaatgaga atgaaaatat gagtttatat gtcagtaagt
    20401 taaaagaagt attgaaaaat aattcagaag agtataaaaa aataagagct aaaataaatg
    20461 aaatttataa tctaaatgaa aagaaagaat aaaagggctt tagctctttt attctttctt
    20521 agtattaact gctttataat aagcattatc tatcattttt tttattgcat ttttatcatc
    20581 tgaatctaaa gatgctaatc tatttattag ttcgttaact tcattatctt cttttgagtt
    20641 aacttttttt aataattcat catcttgttg agtgttaggt tgtattttac ctaatttagt
    20701 taatagctcc tttaaagtta aatttaatcc aaaactcatt ttttcaagca tatctaaagt
    20761 aggttcaaca gctttaccat tccttgggtc tctgtttttt tctatcttat ctatatatgt
    20821 atggctcaca ccacaaagat tagaaaattc tctaagagat aagttatgtt cacttctata
    20881 ttctttaata atttctgcta atgtttgcat aatttcacac ctcatctaag taatagttga
    20941 cttaattata tcagaaaaat gtaaactatt gttaacaaaa ataaaaattt agtaaaaaat
    21001 aattgacaag agtaaaaaaa agacgtatac tatgattaac aaaaaggtta taaataaagt
    21061 taggagggat taatataaaa aataatttac aatatattag aaaagaatct agaatatcac
    21121 aaaaaaaatt cgctgataag ataggtatat caagaccgta tttatctaga atagaaaacg
    21181 gtaaggttaa tccgagttta gaaatagcgc ataaaatctc gctcgaaaca gggaaaacaa
    21241 taaatgaaat tttttttgat tttactgtaa atcatagtaa actataaggt tttaattgta
    21301 ccttgaaaac taaatataaa atattttaaa ggagcaagta tatgaaaaat aaagaaaaag
    21361 aaataatcat tagaggaatt ttaataggaa ttttttattt tttaggaatt gtatttagta
    21421 atactttttt taaataataa atagaagggg taggtgaaat accaactccc caaatcaaaa
    21481 taacactttg gaagttaaat ataaaatatt caaaagaggt gattagatgc aaagagagca
    21541 aacaacgctt cgtataccag aagacttgca caaagcgttg atagatttaa gcagtgatat
    21601 aggaatgcct attacatcta ttataataat tgcatgttgg ttgtatatat caaagataaa
    21661 ttaaccaatc cgatatgcaa aatgaatgca caacattatt tattagagaa ttgattgata
    21721 aattattttc tttagctgtt ttctctagca actcttttag gacaccagga atcctaagag
    21781 tagaacgaac agtatcatca cttttatacg agattgattg aagttcatta acttttgaat
    21841 ttctaataat atcattaata gcgtatagaa taagcgaaga tttgagaatg cctgtttgat
    21901 aagaaatttt ttctaaattt tcatttaatg gtgtatatat tcttacagta atagtagtca
    21961 ttttgacacc tccctaatga cattatacaa aaaatattta aaaaagtctt gacactaaaa
    22021 tagtgtcata atataatgat tatagacagt aaaacagtgt cataaggagg gaggaataag
    22081 aatggaaaaa gtaagaatga cagtaaggct cgtcccaaaa ttaaatcaat atgtacagaa
    22141 tatagccaaa gaatgtggaa aaagtaaaaa ttctattatt gtggatgcat gttgggagtt
    22201 tattgagaaa ataaaaaaag aaaataaatt caatgtagaa agcgaggaat aaatatgaat
    22261 aatctacaga tatttgaaaa attggaattt ggacaaataa gaatggcaga gattgataat
    22321 aagccttact ttgtaggaaa agatgttgca aagtcattag gttataaaaa tactaatgat
    22381 gcaattttaa gacattgcaa aggggtagtg aaacacgagg ggtttaaaat taatggtatt
    22441 aagattgctt tgataactga gggtgatgtt tatagattaa tagtaggttc aaatctacca
    22501 aatgcagaaa agtttgagag ttgggtgttt gatgaagtgc ttccaagcat aagagaaaca
    22561 ggtcaatacc aagcacaaca gcatgtaata acagaactta caggaactat aggagactta
    22621 aaaggaacaa taaaagaata taaaaagttg tgtaagataa catgctctaa gaaacaacag
    22681 tattctaaat acataaagaa tagactggat atagacaaag ctaataaaga atacaaccaa
    22741 gtgaaagcaa ggctattcct aattcttgga gttgagaagt gggaagacat agacttcgat
    22801 acatctaata atttaataca aattatagat gattgtataa gagttataaa gtcagagaga
    22861 ccatacaagc agttgagtta ttttgaaaac taaatacaga atattttgaa atatattgtt
    22921 ttaattaatt attaactagg aggttaaata catgaaaaat aatataagcg attcaagagt
    22981 aaaatatttt tgcaagtgtc catactgtgg gtttgataat gaggtagaag ttaaaaaagg
    23041 attgaagcct aaaatatgtt gcatatgtac aaaagaagtt gagtatgaaa aactggagca
    23101 acaaagtact ccagcaaaat aaaattaata aatatgaata tttttacaag aatctaattc
    23161 tttttctaac ttattaacag catggttata ttgcagtgcc tgttctgcta taaagttaga
    23221 atttgatatg tgttctttac acatagggca tggtagccca ttatcacaaa cttccctaaa
    23281 tttagattct gttatggtaa cttttgtacc acaatcgaaa caagtagctt caattttagt
    23341 tattttaccg atattcataa atcaccacct aatcatatat ttataggatt tatcctacaa
    23401 atatagtata tcaaaggagg gaaataatgg caattaatga caacataaat aaaattttaa
    23461 gagatagaaa tttaaaagcg tggaaattag caaaagaaat aggcgtagat tcaggaaatt
    23521 tatatgcaat tttaagagga gaaaataaaa atccaactat agatacattg ataaaactag
    23581 ctgactattt agatgttaca ttagacgaat tagttggaag atagaaatta aatacagaat
    23641 attttgaaag taggtgtatt gtatggcaaa agcagtagct aaagaacaat tgttttatag
    23701 ggcaaaagat atagctaaga ttttagatgt atgtgaagca accgcttata aaataattgg
    23761 agaattaaat aaagaattag aaaaagaagg ttttaagact ttttctggca gggtatcagt
    23821 tgcttatttt aaagaaagat attgttataa gccaagaaag ggggtgattt agttgaatgt
    23881 aaaaggtact aatagcttat atacagtttt gcaatgataa gcaaataaaa gcaagttttg
    23941 aaggtcttag aaaatacaac agagatatag agttagttaa cttgtattta ccaagtaaat
    24001 taaaaataaa taaggaggtc taattgtata tggtatttaa tttagagaag ttcaaagtgg
    24061 gaaatgcgat aagaataagt tgtgaaagat ttggttttga aattgattgt attgtagtag
    24121 tagcaacaga aaaagaatta aatttagcat actttgatga aggtagaggc tgtatggagt
    24181 atcaagcctt aataacagaa gatattcaag atggtgatta tgagattaaa attttatctt
    24241 aggaggaaat aaaatggcag ctttaataat ggtaggttta tttgcaatat gtttagtagg
    24301 attagtacaa aatagagatt aagtgaaggg gtgtatttag atggaagcag ctagattaat
    24361 agcaatgggt caaattaaac aggctgaaaa agaaataagt aaattgcaag gtacaaaaaa
    24421 caatagtagt ttaatgtggt gggaagccgt aaaatttgct agtcaaaata tattacaagg
    24481 gttggaacat gatatagagc tagaagcatc aactgatttc agggagttta tgataacaca
    24541 agaagaactt gaaagagata gacctataga tgtgcagata taagaaaaga gcctagggtg
    24601 aggctcaatt caaataaata ttaaaaaatt taattaagct aattatagca taaacggagg
    24661 gaaattatga gtactttata tgaattaact acagatttat tagaaataga agaaggttta
    24721 acagaaacaa caggaaatga agctgaaaaa ctagaggaaa taaaagaaat aataaaacaa
    24781 gagatacaaa ataaaaacac taggatagtt tcagtaatat taaacattga cagtgatata
    24841 aactctatag attcagagat taaaagattg caagagttaa aaagggtcaa aaagaatact
    24901 cttgatagat taaaaagcaa tataaaagac tgtatggaat tacttggtac taaaaaagta
    24961 gaaacagttt taggaaatat aagtataaga aagtcagcag gtagcttagt catagaagat
    25021 gaagaaaaga tacctgctat atataaaaca gtagagcaag ttgtaaaagt agataagaat
    25081 accattaaag attttattaa aaaaggtcat gaggttgaag gttgcaggat tgaatatgga
    25141 actacactaa caattccaaa agctaaaaaa gagtaggtga ggaccatgga aattaataat
    25201 atttacatta aattgatgga tgtaagagtt aaatttagta agttgaatct aaagaaaagt
    25261 ggagaaaata agttcgctaa cttcaagtat tttgagttag cagactttct accacaagca
    25321 actggattac ttgaagaagc taagctatgc cctatagtga cctttacaaa tgaatatgca
    25381 actctaacat taattaatgg agaaaaccca tcagaacaga ttgtatttac ttctcccatg
    25441 agagatttac aacttaaagg ttctaatgaa ttacaggcac taggaggtat agaaacctat
    25501 caaactagat atttgtatat tcagttacta aatataactg aaaatgatag ttttgacgca
    25561 gtaagtggaa aagaggaatc taaaagcaat tctaataaca atgttaaaca aagtaacaga
    25621 atattgactg ataaacaact aactagatta tatgcaatag ctaatagtgt tggagttaat
    25681 aaagcattat taaaagaaca ggtttttaaa cagctaggaa aagatataaa ggatttaaat
    25741 aagagtgaat atgatgctgt ttgtgcagca tatgagaaac aagcctttaa gggtgcataa
    25801 caggaggtga tagagtggca gttttcagac agatatatac aagcttttgg actgacccaa
    25861 aggtacaaga ggagtggaca ccagaagata aatttttctt tatacttctt cttagtaatc
    25921 ctcaaacaac acaaatagga gtttaccaag taactaaaaa acaactagca ttttggatgg
    25981 ggtactcaga agaaagtata agagcattaa tggatagatt cgtaaatcac cataagtgta
    26041 ttaaatataa tcccgatact agagaaattg ccataaaaaa ctggggcaaa tataacttaa
    26101 ctaaaggtgg taaaccaatt atagatttac ttaataaaga actcaaagaa gttaaagata
    26161 ttgagttaat aaaatatgta attccatcaa tagaaaaatc agatataaga aaaatatttg
    26221 aggattatta cgaaatggct aaaaatgggg attcttacga tacgtcgacg atacgtgaac
    26281 gagtcgtacc acgatacggg gacaataaca ataagaataa caataagaat aacaataaga
    26341 ataacaacaa caataaggat gattcggtgg ttgtggataa aataaaacaa tactttgatt
    26401 tagaatctaa agacattgaa aaaattgttg atatattcat acatacagga agaggaattg
    26461 actatttaga ggaaaaacta aggttggtaa aaaatacaga tggagtaaaa agtattacag
    26521 gctatctcat aaaagcatta gaagaagatt ataagcctat accaagtaga aaaaataaga
    26581 ctaagtttca taattttaac gagacattta ctaagtatac gtctgatgaa ctagacgaga
    26641 ttattaaaaa gagtcaaaaa gagaagttca gttgaattaa atagtctagg gagtagttat
    26701 acaatattac ttcctaggag ttaaaaaata ttggagggat agaaatgata ataattagaa
    26761 gtcaagatag attagattta atgagagtta atagagttga aataggtttt aatcaagtgt
    26821 atgcaatact tgaaaatgat attagaaaaa taggtgaata tgaaagtatt gaaagagcta
    26881 tgcaagtgtt aaatgatata cagaagttta ttgaaaatgg agtaagaaca gattatatag
    26941 attcttgtag agttagacat aatcaagaga aagtatttga aatgccattt gaataaaaag
    27001 gggggaacta aatatggcta aggtttgggt ggatgcagga acatttttag aaagaactaa
    27061 ggatatagaa gatatgtttg aatttaatct aagaaaagta agagatagaa ataaaaaaat
    27121 aaatgtaatt gatttgcgtg taaataatga ggggcaagga agaaaaggta aaaaagtaga
    27181 gtgttttaat attgtgactg gagaaactaa agtatttaat actgctgttg aagcaagtaa
    27241 atacgtatat tttacagatg tttatattgc tcatttagca agaacaggta aggtttctaa
    27301 aaatggatgg aaagctagat atattcaaga ggtgacagat ggtattggca aatgtggaac
    27361 aagtaattaa gttagctgaa aagatattaa ataagaaaaa gtgttctgtt aataaagcta
    27421 ttgatatagt tataaaaata ttaagtaaat atgagtgcga ggggatgtta agaaatgagt
    27481 ttaattaagt atagaggtta tgattttgag aacgaaaagt ggatttattc agcaacaata
    27541 atgtggagca atatacttga atgtttagtt atgttgacag agggttgcaa atggcagaaa
    27601 gtctctaatg ttggggtatg ctctggagaa tgggctagaa acaatcaaga aatttgtgag
    27661 ggggatatat tgaaaggata tgataatttc catgacacaa gccaatatgg agttgtaaaa
    27721 agggatattg gtagtattaa gttatatttg gaatggcatt atttaaagaa acttgaagga
    27781 gaatggatag agcttataaa taaaacagaa atatatcata gcagagatta caaaatagtt
    27841 ggtaatgaat atgagaactt agaggaagtc agaacagagt tcttagaacg taaggagagt
    27901 cttgagaatg aatatcttag ctagtgcgat attagtaata ggaagtttta tagctggtag
    27961 agtttatgag tatagattga atctaaaaga gtgtgaaaat tgcgacaata aaagaggtgt
    28021 ataagaatgg atgatagatt agaaatgata aatgcttctg taaattatat acagatgata
    28081 tgtgaaagtt caaatatagc tattatagca gagcgaggaa gagttagaat attagattta
    28141 gaaactaaag aaaaatatga tttattaaaa aataaactcg aggaaatgtt agaagaaata
    28201 taagtgaaaa tatctaatta agacagttta gagagttaca aaatatcttt tagtataaat
    28261 tattgttgaa gtgttttgt
//
LOCUS       DAEPIN010000054        27859 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.054, whole genome shotgun sequence.
ACCESSION   DAEPIN010000054 DAEPIN010000000
VERSION     DAEPIN010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27859
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.054"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            145..1626
                     /locus_tag="KR250_002579"
     CDS             145..1626
                     /locus_tag="KR250_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HBH0068507.1"
                     /translation="MKKNRLIAKNISIAFIVLFFFSVFTFFYVGNINRVLEYETNDII
                     TVTIAGWIILSFLFLGIIIYILYSKANSQKTIEKVAYTDFVTGYSNWRKFELDVTNLL
                     KKTSQNNKYAMVIFDIDKFKAINDIYGHKKGNLILKDIADTLNELTDINETFARVSAD
                     NFNILLTYNKKEDIINIIKKIMANNELVNLSFGIYEIKDKDLSVSVYSDRASLAKSSI
                     KNNSDVNFAFFNDKLREKLLFEDKIEKEMEYALESGQFVMYLQPKYNIKLDKFCGSEA
                     LVRWQYTEKEVIYPGDFIPIFEKNGFIRKIDMYILEQACKEIRSLFDKGISPLPISVN
                     FSRVDFFKKDFIENIVNICDRYKIPYSLIEIEITESSMFGDTDTLFNVSRNLQDIGFI
                     VAMDDFGSGYSSVNMLKNIPLNVIKLDRGFFVYDKDVDKSQIVIKSIVSLIKQLGIRV
                     VAEGIETRSQIEMLKKANCDIVQGYYFSKPLPIKEFEKLVYKI"
     gene            1659..5096
                     /locus_tag="KR250_002580"
     CDS             1659..5096
                     /locus_tag="KR250_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HBH0068508.1"
                     /translation="MIRAKSLKNVLSIVLIFLFTIISFSDFAFKTNVQEEENSKINLR
                     EISLQSSNAIKIKLDSSLSFIKSVANMVSYFNTIDNSKIVPLLANISKESDFQRISIV
                     SPDGISHTSDGKIINVSKYQYFKKAIKGESNISEVVKSPIDGKDTFVIATPVFNKNNK
                     IIGVLYGSYYNDKLAEFIDITSFNSEGYIDIFESSGQFVLKSSHTNKLIKSSSNIYDI
                     FKKASFDDGYSYKKIYEDTKNNKSGFTSYEYKGENRYATYTPIGVNDWYVISVIPQYT
                     VRRNIDKINQLAFSLIFKVFLLFIMLITYIIYKNNKSQKDILKANKEISSITNSIPGG
                     VQKCTLGETCNINFLSDGFIELFGYTREEIKTLFNNDFYSLIHKDDVERIKKEIFKQL
                     YKNKTVELEYRIITKNGNTVWVLDKGELITEPDGKEYFCCVTVDITRTKQLEQEMEQK
                     NLELEMISENVLGGIIITEFNDDFTIKYANSGYLSMIGYTREQLENELKYKACNLVFY
                     EDRKKALESIQHQLNTKGIAVYQHRIRRRDGNIIWVSIKGRLIVDDDGIEKGIWILND
                     ITDLKNAEQKLKINEERFRIALKQTSNTIFEYDIKTKSLLNTSKELDKYNIYKITKNV
                     PYSLVELGVIHKDFSDDFINMYENVINGAKKASCVVKMKLPDENYIWNKVTLTTIFDE
                     DGNPVRAIGIQEDISKQKEAEIRYNQEGKYHSAILSEAMITYEINVSKDTFIKGNESW
                     QKPFGIKPDNNYSQMVKTIANSIVHKDNKETFLNTFLCENLLDAYKNNVTEIKLEYRR
                     MNQENKMIWVICTMHLIKDSITNDIKALAYVKDIDKQKNEEIRLKLKAERDTLTGLYN
                     KGTTEKLINDFLNSEEASLGMHAFLIVDIDNFKSVNDNLGHVFGDKVLFEVSKNLKPI
                     FRKDDIVGRIGGDEFIVFLKNIQSFENIKKKAEKICDIFRNSYTENKKTYKISGTVGI
                     SFVPEHGTVFEELYKKADIALYYAKSKGKDKYALYNDCIYQNELENNISDNNIKLADK
                     CEKSKPVLKEILDSIDNYIYIVNPNTYELLFVNNKVSRLDLEIKLGETCYKSLLKSDR
                     PCNNCPLNGLEENKHSKFKIETNTFGKNISIASWINCIDGEYNCLIECIDSSLYKNK"
     gene            5114..5860
                     /locus_tag="KR250_002581"
     CDS             5114..5860
                     /locus_tag="KR250_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HBH0068509.1"
                     /translation="MLNSITLILGIIFTIYYFYLKIVFGGISFSEIFLVVGIVLIIYQ
                     IFKKKIKEKKVFYRVLKIIISIFLIVFVVTESLIIFYPKNSLESKSDYLLILGASVKK
                     TTPSTTLKGRLDTALKYLKINDNCYVVVSGGKGSGEKITEAKAMKDYLIKNGIDKNRI
                     IEEDKSTNTYENFKYSKLKIEEHSQRKLSDLKVKIVTTDFHVLRSKILAYRNGYKNTS
                     FYASKSKLSFVPTYYTREFFALWKTIIFDR"
     gene            complement(5809..6117)
                     /locus_tag="KR250_002582"
     CDS             complement(5809..6117)
                     /locus_tag="KR250_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HBH0068510.1"
                     /translation="MNFLKENSTFYFATVEDNKPRVRPFGFIMDYEGKLYFGIEKYKT
                     SYKQLLVNPNIEISTTSKVGKWIRIKGIEKTRYEFISSLFIYYLSKMIVFHNAKNSLV
                     "
     gene            6920..11053
                     /locus_tag="KR250_002583"
     CDS             6920..11053
                     /locus_tag="KR250_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF11 domain-containing protein"
                     /protein_id="HBH0068511.1"
                     /translation="MATVSGQIVFDRNRSATIDSGDSGIANIPVVLQDIATGVRLVVL
                     TDASGNYVFTNVPNGNYRIVEAFGTTGGVPTPGNFATAIVGPVPIAEMPPISPPIGST
                     NLDGTTPNTLLITVAGSDITNQNILNGPVIYTPIENILDSCVSVSNINLITDADNGTF
                     GFFPPGTPSNTGPEIEPYPNVTPDFTYVVPDPTKFTPLDGEYTVQNIMTNAMSNVIGA
                     WWRIADHTTGNETGRMMVVNGFNPGAVFFKSTVQVTPNTNYLFSTWILNLFKVTGYPP
                     PQLGVRIFDQNNNILYQATLGAQIPVNTNAPEWKEVGTVINSQNNNQLTVEFFSEGES
                     AIGNDYAIDDVSLREIAVPTFTPIKTSNTETANVGDIITYTVTLDNTCDSPLTNVFFR
                     DLIPNGLEFIPGTVTVDGVSVPGVDPNTGFSLPDIGGGTGVEVTFDVVVESIPNPNPT
                     NNIANIDYSYTPVEGGIPNDFSVDSNPVPVEVISADIEVTKLSEPTIVNPGEELIYTI
                     KVVNNGPFPSENVVLTDDVPASIINPEYSLDGGVTFQPWTGSLNIGTLEVGETRVIII
                     RGIVNPSTVGIITNTAVVSSTTADPNLNNNTSTIETEVNLLADILVMKTAEPNSAVPG
                     TLLRYTIQVENLGPANAENVILNDDIPASIINPEYSLDGGASFQPWNGSLNIGTLNSG
                     ISLTVLIQGTVSLNSSEYIVNTATVSSTTPDPDLSNNISTIITPVNPQAGISIIKVAD
                     EDVAVPGEEFVYTIEIFNEGPSNATNVVLTDDIPDVILNPEYSLDGGATFQPWNGSLN
                     IGTVAPGQLIRIIIKGLVSSTATGDITNTAEVSADVPEPVTDSSTVTTPIVPSADIEV
                     IKTSNMDTAVPEETFSYTITVTNLGPSAAQSIVLTDDIPDVILNPEYSLDGGVTFQPW
                     NGNLSIGTLDAGEIRSIIIRGTVSQTAVGTIINTATISSPTPDPNPDNNTSTDETDIS
                     PLADISVIKGNEPVAIPGGRFIYGIEIANAGPSFAENVTLTDNIPASILNPEYSIDNG
                     VTFQPWNGSLNIGTLDAGEIRNIIIRGTVSQTAIGTIINTATVSSTTPDPNLNNNTST
                     SEAEVSSSADISVVKRSNQTVVVPGDVLDYTIEVRNAGPSTAQNVTLTDNIPASILSP
                     EYSIDNGVTFQPWNGSLSIGTLDAGEIRNIIIRGIVSQTAIGTIINTATVSSTTPDPN
                     PDNNTSTSEIDISSSADISVIKLANKTEACVGEQIDFVIVVSNAGPENAQNVTLIDNV
                     SDKLKKAVFSLDRGVSFQPWTGSLNIGTLPAGTLRVIILRGIIKSTCLDRLTNIAEVT
                     STTPDSNLNNNISRVQVEIKQFCCNDCGCKDCCCNCCCNDCGCKNCCCNCCKNDFWC"
     gene            11510..11857
                     /locus_tag="KR250_002584"
     CDS             11510..11857
                     /locus_tag="KR250_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HBH0068512.1"
                     /translation="MHENKKNLLDGSLAQYNDTAVPKIPVFAGNDITSEVYYFKPNQV
                     LNAHRHPNGEQIFVFLKGEGKMKLGEHECDVKNGDTVFVPTGEWHEITNGSNEEMVAV
                     QITKINAGAEYRG"
     gene            complement(12007..13056)
                     /locus_tag="KR250_002585"
     CDS             complement(12007..13056)
                     /locus_tag="KR250_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBH0068513.1"
                     /translation="MFKKIVAIASSIIVSTICLTGCDFNSNDKKDESSSSKNLDKVTI
                     AEVTHSVFYAPQYAAITKGFFEDEGIKIDLINTQGADKTMAALISGEANVGLMGPEAS
                     IYVYNQNKNDYAVNFAQLTKRDGSFLVARKKMPNFSYNDLKGTEILGGRKGGVPLMTF
                     EYVLKEKGLTIGENLKAGNVNVRTDVQFGVMAGAFAGGEGDFTTAFEPTATAMEKEGS
                     GYIVTSIGKDSGEIPFTAYSTTRSYMKNNKDLIQRFTNAVYKGQLWIQQASSKEVAKA
                     MQPFFEDISLEDLTTVVDRYKSIDAWSQNPVLKEEGLKKLMTVMKEAKELDKEAPYKD
                     IVNTEFANNAIKNIK"
     gene            13442..14176
                     /locus_tag="KR250_002586"
     CDS             13442..14176
                     /locus_tag="KR250_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBH0068514.1"
                     /translation="MKQNKYDDDRFFNKYSKMERSTNGLAGAGEWHVLKKMLPDFKGK
                     RVLDLGCGFGWHCQYAVENGAISAVGVDISEKMLKEARNKTQLDNINYICMPIEDINF
                     PKDSFDVVISSLAFHYIQSFEDICKNISNCLSNKGNFVFSVEHPIFTAYGSQEWYCDK
                     DGNNLHWPVDRYFEEGVRRSNFLGEEVIKYHKTLTTYLNALMKTGFEILEIIEPQPEE
                     KLLDTIPDMKDELRRPMMLLVSAKKR"
     gene            complement(14319..14810)
                     /locus_tag="KR250_002587"
     CDS             complement(14319..14810)
                     /locus_tag="KR250_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HBH0068515.1"
                     /translation="MNNQQQEVYKKLDELSIPYEAVNHPPVYTIEEMEELKTLHMEFV
                     VKNLFLRDAKGKEHFLVVLGKDKKADLKDIRQQIDSKPLSFASEERLQKYLKLTKGAV
                     TPFGILNDKEAVVKVVFDKDLLKMDKIGIHPNDNTATVFLKFEDMKKLIEENGNEIYY
                     VEV"
     gene            15159..15542
                     /locus_tag="KR250_002588"
     CDS             15159..15542
                     /locus_tag="KR250_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0068516.1"
                     /translation="MSTDKVVGIIDEETAELAGIEYTGKIYASSGVIKHIKKKHRCQL
                     SKDIFNDIIDTIKMVLKSPEYIGSHPKKPGKSVEFIKKIDDYILVATELDTKKGYLYV
                     SSMYAIKEAKLESRVNSGRVKQYDG"
     gene            16050..16433
                     /locus_tag="KR250_002589"
     CDS             16050..16433
                     /locus_tag="KR250_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBH0068517.1"
                     /translation="MNLSKKIEDKRKAKQRAEKVKKAKIATAGVVLGAVTGAVSGVLL
                     APKSGKETREDIKDASQQIAEKINMKTVDVKGKVSEKLEDKKGNFIESKKKIKKYIDN
                     KKSVENSSEEDKIIDIVEPVSVTEE"
     gene            16435..16785
                     /locus_tag="KR250_002590"
     CDS             16435..16785
                     /locus_tag="KR250_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0068518.1"
                     /translation="MDNTITINISIIYVLLAILACIALVYLIIVLVKLHKILNNVGTM
                     LNENTQNINTTLNNLPNVVETFGEVGENMKDVTEVVTEVTADFLVTKDSVKSNMDVIV
                     DILTIIKNIFVDKK"
     gene            complement(16886..17344)
                     /locus_tag="KR250_002591"
     CDS             complement(16886..17344)
                     /locus_tag="KR250_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0068519.1"
                     /translation="MIRTKVVELIATVCRENKPHKWVDENYTPYDKSGKVELMSIEDL
                     NELISSSGRADFLYSSRLQKLLNEVYINQSRASYISGCGLFWSSYWDILEEKFEEWLY
                     NSYIFFDEDDEYLEGMEDFELECKDVLMDVIETTSIDIYVQMIKRNITNY"
     gene            complement(17389..18177)
                     /locus_tag="KR250_002592"
     CDS             complement(17389..18177)
                     /locus_tag="KR250_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBH0068520.1"
                     /translation="MKVVIIPGHTLIGKGTGAVGYINESKETRILNDLIVKWLKIGGA
                     TVYTGRVDESSNHLADQCAIANKQETDLAVQIHFNSNATTSTPVGTETIYKTNNGKTY
                     AERVNTRLATVFKDRGAKSDVRGLYWLNHTIAPAILIEVCFVDSKADTDYYVNNKDKV
                     AKLIAEGILNKSISNSQGGGENKVYENVIVYTGDADKVAAQILHWQLKDSLIIEASSY
                     KQGLGKKVYVVGGEANKLVKGDVVINGADRYETVKLALQEIDKL"
     gene            complement(18316..18753)
                     /locus_tag="KR250_002593"
     CDS             complement(18316..18753)
                     /locus_tag="KR250_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HBH0068521.1"
                     /translation="MNKKLENRCSWQEYFMRLCETVAERGTCDRAYVGAIIVNSENRI
                     VSTGYNGSISGDKHCSEVGHEMRDGHCIRTIHAEQNALYYCAKEGISVKNCSIYVTHF
                     PCLNCTKAIIQAGIKHIYYRTGYRIDEYAIKLLQSSNVLYTKL"
     gene            19575..20084
                     /locus_tag="KR250_002594"
     CDS             19575..20084
                     /locus_tag="KR250_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH0068522.1"
                     /translation="MLSENIKTIRKSKGLSQQELAIKLNVVRQTISKWEQGLSVPDSD
                     MLISISEVLETPVSTLLGETIVESEVDNLKVISEKLEIINLQLAQRKNMRRKMFHWLF
                     ILLCAVILIVFVVLGRLNSPYLDWNYTDPETAVFGVAFHAFEWLFVRLVPIVLIGAII
                     GIFLTRKKI"
     gene            20111..20260
                     /locus_tag="KR250_002595"
                     /pseudo
     CDS             20111..20260
                     /locus_tag="KR250_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005342994.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            20498..20929
                     /locus_tag="KR250_002596"
                     /pseudo
     CDS             20498..20929
                     /locus_tag="KR250_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016295744.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(21116..21309)
                     /locus_tag="KR250_002597"
                     /pseudo
     CDS             complement(21116..21309)
                     /locus_tag="KR250_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
     gene            21411..21917
                     /locus_tag="KR250_002598"
     CDS             21411..21917
                     /locus_tag="KR250_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBH0068523.1"
                     /translation="MKNEKKITVALLIVYLLILSWIILFKLQFSFSALDHIRQINLIP
                     FGGSVIANGKIDFDEIINNAIVFIPVGAYFSLLFKNKSVLKAIGSVLGISFVYEIIQF
                     IFAIGASDITDLISNTLGGIIGIALVYVLSIVLKDKTHKILNRIAIVCTVLVVAFLFM
                     LLAVNNIF"
     gene            21922..22198
                     /locus_tag="KR250_002599"
                     /pseudo
     CDS             21922..22198
                     /locus_tag="KR250_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436830.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4865 family protein"
     gene            22467..23039
                     /locus_tag="KR250_002600"
                     /pseudo
     CDS             22467..23039
                     /locus_tag="KR250_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902350.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            23142..23957
                     /locus_tag="KR250_002601"
     CDS             23142..23957
                     /locus_tag="KR250_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HBH0068524.1"
                     /translation="MFKIGEFSKLTQVSTRMLRYYDETGLLKPAKIDSLTGYRMYSAE
                     QISTLNRIIYLRDSGFNVAEIALALNFSNNAIIEQLDRKSIEIMDNIQKEQEKLKKIE
                     IAKKEVLHGKSELYFNITIKEIPSYCVLSLRKEIPNYYYEGELWKELSAFAKENKINI
                     SDETFSIYHNEEYKEKDVDVELCAIVNKIGKATGDFKFRFTEPVSMMACTMVYGDFSN
                     ISYAYRSFAKWLQENSQYKMKGSSRQIVHRGPWNEKNPENYLTEIQIPLENLL"
     gene            24038..24514
                     /locus_tag="KR250_002602"
     CDS             24038..24514
                     /locus_tag="KR250_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002608187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HBH0068525.1"
                     /translation="MTTYKVNSIGTICNNEKGAFIQLEAPYILALRALDGFSHINVLW
                     WFSDFDNTNFRSVLETEQPYKNAPEKMGIFATRSPVRPNPIALSAVEIIDIDYMSGLI
                     QIAYIDANDKTPILDIKPYTPSLDRVENPQVPDWCSHWPHSLEESASFAWESEFNF"
     gene            24528..25091
                     /locus_tag="KR250_002603"
     CDS             24528..25091
                     /locus_tag="KR250_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBH0068526.1"
                     /translation="MSLQDYIFEKPVIETEHLILRTMNKNDIDDLKEWISDISLYEYW
                     GKRPSKSEKNPELLFDKSKKSTKSFHWGIVHKQDSKVIGEIWIYLIENNRMAKVAFRL
                     SKHYQGNGLMSEVLKNVIEFCFSKTELQKIWTDVHILNHASYKTLEKVGFQREGHIRG
                     GKMVNTYCDYYLYGILKSDYITRWCYG"
     gene            25346..26257
                     /locus_tag="KR250_002604"
     CDS             25346..26257
                     /locus_tag="KR250_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBH0068527.1"
                     /translation="MYAYENIEMSLNYIEQHLSEKIETEKLAEIACLSTFYYQRLFKR
                     LVKKSVQEYIKLRRLAMVIAELNNSEERILDIALKYGFSDHANFTRAFKEVYHITPDE
                     YRKNVPMLNTFDKPELSSRYIMIDENVPLIVGNIVLEIQRKTLRTKETYFGFEKTVNI
                     SEQIPAGESTGIDVPGQLWREFHAKKLQTENAIFNNDIELGVSHLASPDKGTFLYFAG
                     GMVSSTDVCPKNMVQHTLPAGEYIVCKIEAESFEELVTTALNQANKYLFETWLPNHNL
                     VTKPFMAEKYYKGNAEINYMEIWIIPV"
     gene            26335..26769
                     /locus_tag="KR250_002605"
     CDS             26335..26769
                     /locus_tag="KR250_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HBH0068528.1"
                     /translation="MINIKDINYVPNISEISVYIENSAFDELYQHMNNEYKAVCKIEY
                     SKDVWARGWNVKFKKSGKSLCVVYPKKNYFTVLVVVGNKEKEDVENLLPRLSEYVQEI
                     YRNTREGNGQRWLMIDLKSKNNVYQDVLRLIEIRYAIKCVKP"
     gene            27237..27859
                     /locus_tag="KR250_002606"
                     /pseudo
     CDS             27237..27859
                     /locus_tag="KR250_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243519.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
BASE COUNT        10533 a         3643 c         4453 g         9230 t
ORIGIN      
        1 tgttatcttt tagatgataa tgatatttat ttaaatactc tttattaaca atttataaaa
       61 cttaattgta aaaaatactt attaatagta aaataaagtg aatcaattat attataattg
      121 ctaaagagag tgaggtgtag ctatttgaaa aaaaatagat taatagcaaa gaatatatca
      181 atagcattta tagtactatt cttttttagt gtatttacat tcttttatgt aggtaatatt
      241 aatagagtgt tagaatatga aacaaatgac ataataacag ttacaattgc tggatggatt
      301 atactttcat ttttattttt aggtattata atttacatat tatattcaaa agcaaatagt
      361 caaaaaacta tagaaaaagt agcatatacg gattttgtaa caggttattc caactggaga
      421 aagtttgaat tagatgttac aaatttatta aagaaaacat cacaaaataa taaatatgca
      481 atggtcatat tcgatattga taaatttaaa gcaattaatg atatatatgg ccataaaaag
      541 ggaaatttaa tattaaaaga tattgcagat actttaaatg aattgactga tatcaatgaa
      601 acttttgctc gtgtaagtgc tgataatttc aatatattat taacatataa caaaaaagag
      661 gatataataa atattatcaa aaagattatg gcaaataatg aacttgtaaa cttatctttt
      721 ggaatttatg aaatcaaaga taaggattta tctgtaagtg tatatagtga tagagcttca
      781 cttgcaaaat catcaataaa aaataatagt gatgtaaatt ttgcattttt taatgacaaa
      841 ttaagagaga aattattatt tgaagataag atagaaaaag aaatggaata tgctcttgaa
      901 agtgggcaat tcgtaatgta tttacaacca aaatacaata ttaaattaga taaattttgt
      961 ggaagtgaag ccctagtcag atggcaatat acagaaaaag aggtcatata tccaggtgat
     1021 tttattccta tatttgaaaa aaatggattt attagaaaga ttgatatgta catattagaa
     1081 caagcttgta aagaaatccg gtctttattt gataaaggaa tttcaccttt acctatatct
     1141 gtaaacttct caagagttga tttttttaaa aaagatttta ttgaaaacat agtaaatata
     1201 tgtgatagat ataaaatacc atatagttta atagaaattg aaattactga atctagtatg
     1261 tttggagata ctgatactct atttaatgtt agtagaaacc tacaggatat tggctttata
     1321 gttgcaatgg atgattttgg ctctggatat tcttctgtaa atatgcttaa aaatatacca
     1381 ttaaatgtaa taaaattaga tagaggattt tttgtttatg ataaagatgt tgataagagt
     1441 caaattgtta taaaaagcat agtttctctc attaagcaat taggaataag ggttgttgct
     1501 gaaggaattg aaacaagaag tcaaatagaa atgttaaaaa aagcaaattg tgatattgtt
     1561 caaggatatt atttttcaaa accattacca attaaagaat ttgaaaaatt agtctataaa
     1621 atatagtcag cgagtcttaa atgggaggtg gtgattaagt gataagagca aaatcgttaa
     1681 aaaatgtact gagtattgtt ttaatatttt tatttacaat aatttctttt tctgattttg
     1741 catttaaaac taatgtccag gaagaagaaa atagtaaaat taatttaaga gaaatatccc
     1801 tacaaagttc caatgctata aaaataaaat tagatagttc tctatccttt attaaaagtg
     1861 tggcaaatat ggtttcttat tttaatacta tagataactc aaaaatagtt ccattattag
     1921 ctaatatatc aaaagaatct gactttcaaa gaatctctat tgtatctcct gatggaataa
     1981 gtcataccag tgatggcaaa attatcaatg tgtcaaagta tcagtatttt aagaaagcta
     2041 ttaaaggaga aagtaatata tctgaagttg tgaaatctcc aattgatgga aaagatactt
     2101 ttgttatagc tactcctgta tttaataaga ataacaaaat aattggtgtt ttatacggtt
     2161 cttactacaa tgataaatta gctgaattta tagatataac aagttttaat agtgaagggt
     2221 acatagatat atttgaatct agtggtcaat ttgttttaaa atcttcacat acaaacaaac
     2281 tgattaagtc aagtagtaac atatatgata tatttaaaaa agcttctttt gatgatggtt
     2341 atagctataa aaaaatatat gaagatacaa aaaataacaa gagtggcttt acctcatatg
     2401 aatacaaagg agaaaatcgt tatgctactt atacacccat tggtgtaaat gactggtatg
     2461 taatttctgt gataccacaa tatactgtaa gaagaaacat tgataaaata aatcaattag
     2521 cattttctct tattttcaag gtattcttat tgtttattat gcttataaca tatattattt
     2581 ataaaaacaa taaatctcag aaagatatac taaaagctaa caaagaaatc tcatctatta
     2641 caaatagcat tcctggagga gtacaaaagt gtaccctggg tgaaacatgc aatattaatt
     2701 ttttaagtga tggatttata gagttgtttg gttatacaag agaagaaata aaaacattgt
     2761 ttaataatga tttttatagt ttaatacata aagatgatgt tgaaagaata aaaaaagaaa
     2821 tttttaaaca actttacaaa aataaaactg ttgaattaga atataggatt ataacaaaaa
     2881 atggtaatac agtatgggtt ttagataaag gggagcttat aacagaacct gatggaaaag
     2941 aatatttttg ttgtgtaact gtagatatta ctagaacaaa acaattagaa caagagatgg
     3001 aacaaaaaaa tctagaactt gaaatgatat cagaaaatgt tttaggtggt ataattataa
     3061 cagaatttaa tgatgatttt actataaaat acgctaactc tggttatctt tcaatgattg
     3121 gatatacacg tgaacaatta gaaaatgaac ttaaatacaa agcttgtaat cttgtttttt
     3181 atgaagatag aaagaaggct cttgaaagta tacaacatca attaaacaca aaaggaatag
     3241 ctgtatatca acatagaata agaagaagag atggtaatat tatttgggtt tctataaaag
     3301 gaaggcttat tgttgatgat gatggtattg aaaaaggtat ttggattcta aatgatataa
     3361 cagatttaaa aaatgcagaa caaaaactta aaataaatga agaaagattt agaattgcat
     3421 taaagcaaac atcaaacact atatttgagt atgatataaa aacaaagtca cttttaaata
     3481 ctagtaaaga gcttgataaa tataatattt ataaaattac taaaaatgtt ccatattctc
     3541 tagtagaatt aggagttatt cataaagatt ttagtgatga ttttataaat atgtatgaaa
     3601 atgtaataaa tggagcaaaa aaagcaagtt gtgtagttaa gatgaaactt ccagatgaaa
     3661 attatatatg gaataaagtt accttgacta cgatttttga tgaagatggt aatcctgtaa
     3721 gagcaattgg tatacaggaa gatataagca aacaaaagga agcagagata agatacaatc
     3781 aagaaggtaa gtatcattca gctatattgt cggaagcaat gataacttat gaaataaatg
     3841 tatcaaagga tacttttatc aaaggaaatg aaagttggca aaaaccattt ggaattaagc
     3901 ctgacaataa ctattctcaa atggtaaaaa ctatagcaaa tagtattgta cataaagaca
     3961 ataaagaaac atttttaaat acatttttat gtgaaaactt attggatgca tataaaaata
     4021 atgtgactga aataaaatta gaatatcgaa gaatgaatca agaaaataaa atgatttggg
     4081 tcatttgtac aatgcactta ataaaagatt ctattacgaa cgatataaaa gcacttgctt
     4141 atgttaaaga tattgacaag caaaaaaatg aagaaattag attaaaatta aaagctgaaa
     4201 gagatactct tacaggatta tataataaag gtactacaga aaaattgatt aatgatttct
     4261 tgaactctga ggaagcaagc ttaggaatgc atgcattttt aattgttgat atagataatt
     4321 ttaaatctgt gaatgataat ttaggacatg tatttggaga taaagtatta tttgaagttt
     4381 ctaaaaacct aaaacctata tttagaaaag atgatatagt aggtagaatt ggtggagatg
     4441 aatttatagt attcttaaaa aatattcagt cttttgagaa tatcaaaaag aaagcagaaa
     4501 aaatatgcga tatatttaga aattcttata cagaaaataa aaagacatat aaaatatctg
     4561 gaacagttgg aatttcattt gtaccagaac atggaactgt atttgaagaa ctttataaaa
     4621 aagcagacat agcactttat tatgcaaaga gcaaaggaaa agataagtat gcactttata
     4681 atgattgtat ctaccaaaat gaacttgaaa ataatataag tgataataat ataaagttag
     4741 cagataaatg tgagaaatcc aaaccagtac ttaaagaaat tcttgattct attgataatt
     4801 atatctatat agtgaatcca aatacttatg aacttctttt tgtcaataat aaggtgtctc
     4861 gtttggattt agagataaaa ttaggagaaa cctgttataa atcactatta aaatctgata
     4921 gaccatgtaa taattgccct cttaacggat tagaagaaaa taagcattca aaattcaaaa
     4981 tagagacaaa tacatttggt aaaaatatca gtatagcaag ttggattaat tgcatagatg
     5041 gagaatacaa ctgtcttata gagtgtatag attcttcttt atacaaaaat aaataaaaaa
     5101 ggactatgaa gatatgttaa atagtataac tttgatactt ggtataatat ttacaattta
     5161 ctatttttat ctcaaaatag tttttggagg aatttcattt agtgagattt tcttagttgt
     5221 aggaattgta ctaattatct atcaaatatt taagaaaaaa attaaagaaa agaaggtttt
     5281 ttatagggtg ttaaaaataa taatcagcat ttttttaata gtatttgtag ttactgagag
     5341 tctaattatt ttttatccca aaaatagttt agaatcaaaa agtgattatc tcttaatttt
     5401 aggagcttct gtaaaaaaga ctacaccaag tacaaccctg aagggtaggt tagacactgc
     5461 attgaaatac ctaaaaatta atgataattg ttatgtggta gtatctggtg gtaaaggaag
     5521 tggagaaaaa ataactgagg caaaagctat gaaagattat cttataaaaa atggtataga
     5581 taaaaatagg ataatagaag aggataaatc cacgaatact tatgagaatt ttaaatattc
     5641 aaaattgaag atagaagaac atagtcaaag aaaactaagc gatttaaaag taaaaatagt
     5701 aacaacagat tttcatgttc tcagaagtaa aatattagca tatagaaatg gatataaaaa
     5761 tacgtctttt tatgctagta aatcaaaact ttcatttgta ccaacttact atacaagaga
     5821 attttttgca ttatggaaaa caatcatttt tgatagataa taaataaaaa gactagatat
     5881 aaactcatat ctagtctttt ctattccctt aattctaatc cattttccaa ctttacttgt
     5941 tgtagaaatt tcaatattgg gatttacaag taattgctta taagaagttt tatatttttc
     6001 gattccaaaa tataattttc cttcataatc cattataaat ccaaatggtc ttactcttgg
     6061 tttattatct tcaactgtag caaaatagaa tgttgagttt tcttttaaaa aattcattat
     6121 tttttcatga ttaaatcctc tttgtatctt atactaatat aatgaatata aataaaatat
     6181 tcattatact taatataaat cgatataaaa acaattacaa tattatattg attattcgaa
     6241 attatatata aaattcagaa aaaattttag tttacataac aaaaataaag tcaactaaaa
     6301 ttattgattt tagattaaag taccggatta agcatttaag tagattttaa gcttgatttg
     6361 attggaaagg tacaaactat caccgaaaaa aattaaaccc atttaaaaag caatataaaa
     6421 gcttgttatt tttgagaaaa atagaccctc taaactcaat tagattaaaa ataaaataca
     6481 gaaaattata tctctaaata tcttaaataa ttaagtttaa attttacgat attttgtttg
     6541 attatttata atataaaagc atactcttaa tcttcaaaat ttaaaaagag taaaaaataa
     6601 caaaaaaatc actgacattt cactgacaaa ttgtaatttt gttattataa atacattgaa
     6661 aatacttgat tattgaaaaa tggcataaaa taatacattt tatgccattt actattattt
     6721 gatagttttt aacttatgac ataagatatt ttttaacaca tacaataaaa tataaaaaaa
     6781 ttgatattgc aaataaaaaa aatacatatt agatgttata gaaaaaaagc gcacttatag
     6841 gcttaaaatg tcacataaaa gagttttaaa gaataatata ttatgaacac aaattttgta
     6901 atcacaaagg agtgataata tggcaacagt atcaggacaa atagtttttg atagaaatag
     6961 aagtgctacc attgattctg gagactcagg aatagccaat ataccagttg tattacaaga
     7021 tatagcaact ggagttagac ttgtggttct cacagatgca tcaggaaatt atgtttttac
     7081 taatgttcca aatggtaact atagaattgt agaagctttt ggaactacag gaggagtacc
     7141 aacaccaggg aattttgcaa cagcgatagt aggtccagtt ccaattgcag aaatgccacc
     7201 tattagtcct cctattggtt caacaaactt agatggtaca acacctaaca cactattaat
     7261 aacagtagca ggttcagata taacaaatca aaatatatta aatgggcctg tgatttatac
     7321 acctatagag aatatattgg attcttgcgt atctgtatct aatataaacc ttattacaga
     7381 tgcagataac ggaacattcg gtttttttcc accaggtaca ccaagtaata caggacctga
     7441 aatagaacct tacccaaatg tgacgccaga ttttacgtat gttgtaccag acccaactaa
     7501 gtttacacct ttagatggag aatatacagt acaaaatatt atgacaaatg caatgagtaa
     7561 tgttatagga gcttggtggc gtattgctga ccatactaca ggaaatgaaa caggaagaat
     7621 gatggtggta aatggtttta atcctggagc tgtatttttt aaatcaacag tacaagttac
     7681 acctaacact aattatttat ttagcacttg gattttaaac ctttttaagg taacaggata
     7741 tccaccacca cagctaggtg taagaatatt tgaccaaaat aataatatac tatatcaagc
     7801 aacattagga gctcaaattc cagttaatac aaacgcacca gaatggaaag aagtaggtac
     7861 tgtgattaat tctcaaaata ataatcaact tacagtagaa ttttttagtg aaggagaaag
     7921 cgccattgga aatgattatg ctatagatga tgtttcttta agagaaatag ctgttccaac
     7981 ttttactcca ataaaaacaa gtaacacaga aactgctaat gtaggtgata taatcacata
     8041 tacagttaca ttagataata cttgtgatag ccctttaact aatgtatttt ttagggattt
     8101 aattccaaat ggtttagagt ttatacctgg aactgtaacg gttgatggag tatctgtacc
     8161 aggagtagac cctaatacag gtttctcact tccagatatt ggaggaggaa caggggttga
     8221 agtaactttt gatgttgtgg ttgaaagtat tcctaaccca aatcctacta ataatatagc
     8281 taatatagat tactcctata cacctgtaga aggcggtata ccaaatgatt tttcagtaga
     8341 ttctaatcca gttcctgttg aagtaatatc agcagatatt gaagttacaa aattatctga
     8401 acctactata gtgaatccag gagaggaatt aatatacact ataaaagtag taaataatgg
     8461 accattccca tctgaaaatg tggttttaac agatgatgta ccagcttcta ttataaatcc
     8521 agagtattca ttagatggag gagtaacttt tcaaccatgg actggaagtt taaatatagg
     8581 gacattagaa gtaggagaga caagagttat tataattagg ggtattgtaa atccatcaac
     8641 agtaggaatt ataacaaata cagctgtagt atcttcaact acagcagacc caaaccttaa
     8701 taataataca tctacaatag agacagaagt gaatttatta gcagatattt tagtaatgaa
     8761 aacagcagaa cctaattcag ctgttccagg tactttattg agatatacta ttcaagtaga
     8821 aaacttagga ccagcaaatg ctgaaaatgt aatattaaat gatgatattc cagcttctat
     8881 tataaatcca gaatattcat tggatggagg agcaagtttt caaccatgga atggaagtct
     8941 taatatagga actttaaact ctggaatttc attaacagtt ttaattcaag gaacagttag
     9001 tttaaattct tcagaatata tagtaaatac agcaacagtt tcttcaacta cacctgaccc
     9061 agatttaagc aacaatatat ctaccattat tacaccagtt aacccacaag caggtatttc
     9121 tattataaaa gtagctgacg aggatgtagc tgttccagga gaagaatttg tatatacaat
     9181 tgaaattttt aatgaaggac catctaatgc tacgaatgta gttttaacag atgatatacc
     9241 agatgttatt ttaaatccag aatattcatt ggatggagga gcaactttcc aaccatggaa
     9301 tggaagtctt aatataggta cagttgcacc agggcaatta ataagaatta tcataaaggg
     9361 attagtaagt tcaactgcta caggagatat tactaataca gcagaagtaa gtgcagatgt
     9421 accagaacca gttacggatt catcaacagt gacaactcct atagttccat cagcagatat
     9481 agaagtaata aaaactagca atatggatac agcagttcca gaagaaactt tcagttatac
     9541 tataacagtt acaaatttag gaccatctgc tgcacaaagt atcgttttga cagatgatat
     9601 accagatgtt attttaaatc cagagtattc attggatgga ggagtaactt tccaaccatg
     9661 gaatggaaat cttagtatag gcactttgga tgctggagaa ataagaagta ttataataag
     9721 aggaacagtg agccagacag cagtaggtac aattataaat acagcaacaa taagttcacc
     9781 tacacctgac ccaaatccag ataataatac ttcaacagat gaaacagata taagtccatt
     9841 agcagatatt tctgtgataa aaggcaacga accagttgct ataccaggag gaagatttat
     9901 ttatggaatt gaaattgcaa atgcaggacc atcttttgca gagaacgtaa ctttaacaga
     9961 taatatacca gcttctattt taaatccaga gtactcaata gataatggag tgactttcca
    10021 accatggaat ggaagcctta atataggtac tttagatgct ggagagataa gaaatatcat
    10081 aataagggga acagtaagtc aaacagcaat aggtacaatt ataaatacag caacagtaag
    10141 ttcaactaca cctgacccaa atctaaataa caatacttca acatcagaag cagaggtaag
    10201 ttcatcagca gatatttctg tagtaaaacg aagcaatcag actgttgttg taccaggaga
    10261 tgtactagac tatacaattg aggtgagaaa tgcaggaccg tctactgctc agaacgtaac
    10321 tttaacagat aatataccag cttctatttt aagtccagaa tattcaatag ataatggagt
    10381 gacttttcaa ccatggaatg gaagtcttag tataggcact ttagatgctg gagagataag
    10441 aaatatcata ataaggggaa tagtaagtca aacagcaata ggtacaatta taaatacagc
    10501 aacagtaagt tcaactacac ctgacccaaa tccagataat aatacttcaa catcagaaat
    10561 agatataagt tcatcagcag atatttctgt aataaaatta gcaaataaaa cagaagcatg
    10621 tgtaggagag caaatagatt ttgtaatagt agtatcaaat gcaggaccag aaaatgctca
    10681 aaatgttact ttaatagaca atgtttcaga taagcttaag aaagcagtat tttcacttga
    10741 tagaggtgta agtttccaac catggactgg aagtcttaat ataggtacac taccagctgg
    10801 aactttaaga gtgattatat taagaggaat tataaaatca acatgtcttg atagattaac
    10861 aaatattgca gaagttactt caacaacacc agattcaaac cttaacaata atatatcaag
    10921 agtgcaagta gaaataaaac aattttgttg taatgattgt ggttgtaaag attgttgctg
    10981 taattgttgt tgcaatgatt gtggttgtaa aaattgttgc tgtaattgtt gtaaaaatga
    11041 cttttggtgt tgattttata aaataatttt gtatacctta aattaaactg aaataaaaaa
    11101 taaattcaca tatgagtaaa atattttata aatattaata aattttctat ctttgatatg
    11161 gaccacattc gtaatactag ttattaaatg aagtggtcct ttttaattca ttaaaattat
    11221 ttattctaat taaattttaa atgtattaaa taattttaat tttttataaa aaatattcat
    11281 atgttattta tattaaagtt tagatatcta aaaatacttt tagaatttat tatattatgt
    11341 taatttttta tatcttataa ggattgtatt tttctaacta taaaaatagt attatactta
    11401 tatttaaaaa taaaagaatt ttattcctat tgtgtaaagt gagaatacta ttttcttata
    11461 ttggtataat aatagtaaat ctataatata gaaaagaggt atttctagta tgcatgaaaa
    11521 taaaaaaaat ctattagacg gaagcttagc tcaatataat gatacagcag ttccaaaaat
    11581 accagtattt gctggtaacg atataacttc agaagtttat tattttaaac ctaatcaagt
    11641 tcttaatgct catagacacc ctaatggtga acaaatattt gtgttcctaa aaggagaagg
    11701 taaaatgaaa cttggtgagc atgaatgtga tgtaaaaaac ggagatactg tatttgtacc
    11761 aacaggagag tggcatgaaa ttactaatgg aagcaatgaa gaaatggtag ccgttcaaat
    11821 tacaaaaatt aatgctggag cagaatacag aggatagata tataagtaca gttagtactt
    11881 taatatctat tttattatat aaactttaat ctcaaatata aaaagagtct tttaaaaggc
    11941 tctttttata tttttgttaa ttttcattat aaaaacaaga aaatatctag ctaaaactaa
    12001 tatattttat ttaatatttt ttatagcatt gtttgcaaac tcagtattta cgatatcttt
    12061 ataaggtgct tccttatcta gttctttagc ttctttcata acagtcatca atttcttcaa
    12121 accttcctct tttaatacag gattttgaga ccatgcatct attgatttat atctatctac
    12181 aactgttgtt aaatcttcta aagatatatc ttcaaagaaa ggttgcatag ccttagctac
    12241 ttctttgcta gatgcttgtt gaatccataa ttgaccttta taaacagcat ttgtaaatct
    12301 ttgaattaaa tctttattgt ttttcatgta tgatctcgta gtagagtatg ctgtaaaagg
    12361 tatttctcca gaatcttttc ctatagatgt aactatatat ccactacctt ctttttccat
    12421 tgctgttgct gtaggttcaa atgctgttgt aaagtcaccc tctccacctg caaaagctcc
    12481 agccattaca ccaaattgta catcagttct tacattaaca ttgcctgctt ttagattttc
    12541 tccaatagta agaccttttt cttttagaac atattcaaaa gtcattagtg gaactccacc
    12601 ttttctacca cctaatattt cggttccttt taaatcattg taagagaaat taggcatttt
    12661 tttcctagca actagaaaac taccatctct ttttgtcaat tgagcaaagt tcactgcata
    12721 atcgtttttg ttttgattat atacatatat agatgcttca ggacccatta aacctacgtt
    12781 tgcttcacct gaaatcaaag ctgccatagt tttatctgca ccttgggtat ttataaggtc
    12841 aatctttata ccttcatctt caaagaatcc tttcgtaata gcagcatact gtggagcata
    12901 aaaaacagag tgagtaactt ctgctatagt tactttatcc agatttttag acgaagaaga
    12961 ttcatctttt ttgtcattag agttaaaatc acatccagtt agacatattg tacttacaat
    13021 gatagagctt gctattgcga ctattttttt aaacataata aatacctccc ataagacata
    13081 cgaatagtat atagtattaa atatttattg tagttgttac aaaaatgtat ctatacttat
    13141 aatatttcta caaaaactta ataataattt tcagttttat gtttagtttt ttcagtaatg
    13201 tgtaatattg gataaattat attgattatt tataaactaa gtaataaaat taaaataata
    13261 tcactttttt gtctattata tataaggaaa aattttttaa taatataaat catattcatt
    13321 tatattatta aaaaatttgt ttatagaaac ttttattatt ttatataaaa gtgataaaaa
    13381 cttatatttt gtatttaata gagtgttgct aagtgaatat atttaaaatg gaggaaactt
    13441 tatgaaacaa aataaatatg atgatgatag attttttaat aaatacagta aaatggaacg
    13501 ttctacaaat ggattagcag gtgctggaga gtggcatgtt ttaaaaaaaa tgttacctga
    13561 tttcaaagga aaaagagttt tagatttagg atgtggattt ggatggcatt gtcaatatgc
    13621 agttgagaat ggagcaattt ctgctgttgg agttgatatt tcagaaaaaa tgttaaaaga
    13681 agctagaaac aaaacgcaac ttgataatat aaattacata tgtatgccta ttgaagatat
    13741 taattttcca aaagatagtt ttgatgtagt tattagttcc ttggcatttc attatattca
    13801 atcatttgaa gatatatgta agaatataag taattgtctt tcaaataaag gtaattttgt
    13861 attttctgtt gaacatccta tatttactgc atatggaagt caagagtggt actgtgataa
    13921 agatggaaat aatctacact ggccagtaga tagatatttt gaagaaggag tacgtaggtc
    13981 aaattttcta ggagaagagg ttataaaata tcataaaaca ttaacaactt atttaaatgc
    14041 gttaatgaaa acaggatttg aaatattaga aattattgaa cctcaaccag aagaaaaact
    14101 gttagataca attcctgata tgaaagatga gctacgtaga cctatgatgt tacttgtatc
    14161 tgcaaaaaaa agataagtaa atataaataa tcattattgt agaaaaaaag ttgtagaaaa
    14221 taattacact aatggagatg attataccag tggtattaat tacaccagta gtaaggaggc
    14281 aaaagcattc gttgcttttg ccatttttta agttgcaatt atacttctac gtagtatatt
    14341 tcgtttccat tttcttctat aagctttttc atatcttcaa attttaaaaa tacagtagca
    14401 gtattatcat ttggatgtat acctatttta tccattttta ataaatcctt atcaaataca
    14461 acttttacaa cagcctcttt atcatttaat ataccaaaag gagtcacagc tcctttagta
    14521 agtttcaagt atttttgaag tctttcttct gatgcaaaac taagaggttt actatcaatt
    14581 tgttgacgaa tatcttttaa atctgctttt ttgtctttac ctaatacaac taaaaaatgt
    14641 tcttttcctt tagcatcacg aagaaatagg ttttttacaa caaattccat atgtaatgtt
    14701 tttaattctt ccatttcttc aattgtataa actggtggat gatttacagc ctcataaggt
    14761 atgcttaact catctaattt tttatatact tcctgttgtt gattgttcat gatttcctcc
    14821 cattagaaat ttattttata tataaataat attatcacag aaaactttaa aagaaaacgt
    14881 tttatataat atagacaata gattatattt ttataaagaa gcaagaatac tcaataaaac
    14941 ttaaagaaat taaacgattt ctaaaaatac cttgaaaata gaaccttatt tgccatttat
    15001 aaattatata aaaagggatt atataaagtc atatatgaaa aaactatatt atactatctt
    15061 ttctttttgg aataaactat actttaaata tagatattta atttaaataa attttacagt
    15121 attaatagta aattatctta gaaaggaatg tgctttatat gtctacagac aaagttgttg
    15181 gaatcataga tgaagaaacg gcagaacttg caggaattga atatactggt aaaatttatg
    15241 cttcttctgg tgttataaaa catataaaga aaaaacatag atgtcaactg agtaaggata
    15301 tttttaatga tataatagac actataaaaa tggtattaaa gtcacctgaa tatataggaa
    15361 gtcatcctaa aaaacctgga aaaagtgtgg agtttataaa aaaaattgat gattatattt
    15421 tagtagctac agaattagat acaaaaaaag gatatttata tgtatcttca atgtatgcta
    15481 taaaagaagc aaaattagaa agtagagtaa acagtggaag agtaaaacaa tatgatggtt
    15541 aacataatat tattaatttt ccctaaaata ttgaaaaata ttattatata agttataata
    15601 ttaatataca cgtagatatt tattagttag atttgaggtt ggaaaaggca cccaatacgc
    15661 tactatagag tagttaatag agatgcagga tacgccgccc tgccaaatct aattaaatta
    15721 aaaagagagg attaattcct ctcttattgt ttttttgaga tactaattta atctataata
    15781 gaaagattga aggtcgttta tacaaaaaaa ttttcagtta tatacattaa actaattaaa
    15841 taagatattt ataatctata tgtgatacat attatatatt aataaaagaa aaatatatag
    15901 aaaatcctta taaaaattta aattgtaatg aataggaagt aattaaaaag aatttttatt
    15961 gatttttaaa aatttagtta gaaactatat cattatggta aaaaatgtat tataatgttt
    16021 agaaatatta taataaggag cgattaaaca tgaatctatc gaagaaaata gaagataaaa
    16081 gaaaagctaa acagagagct gaaaaagtta agaaagctaa aattgctaca gctggtgtgg
    16141 tattaggagc tgttactggt gcagtgagtg gagtactttt ggctcctaaa tctggaaaag
    16201 aaacaagaga ggatataaaa gatgcttcac aacaaattgc tgaaaagata aatatgaaaa
    16261 ctgtagatgt aaaaggcaaa gtatctgaga agctagagga taaaaaagga aactttatag
    16321 aatcaaagaa aaaaataaaa aaatatatag ataacaaaaa gagtgttgaa aattctagtg
    16381 aggaagataa aataatagat atagttgaac cagttagtgt tactgaggaa taatatggac
    16441 aatacaataa cgattaacat aagtataata tatgtattat tagctatact tgcttgtatt
    16501 gctttagttt atttaattat tgtacttgtt aaactacata agattttaaa taatgttgga
    16561 actatgttaa atgaaaatac tcaaaatata aatacaactt taaataatct tcctaatgtt
    16621 gtagaaactt ttggagaagt aggagaaaat atgaaagatg taacagaagt agttactgaa
    16681 gttacagcag attttcttgt tactaaagac agtgtcaaat caaatatgga tgttatagtt
    16741 gatatattga ctataattaa aaacattttt gtagataaaa aataataagt taatataaaa
    16801 taagcctata agatataatt taaacgacct taaaatagca tttataaggt cgttttttat
    16861 tagcttgatt tgacactatg tgtttttaat aattggtaat attcctcttt atcatttgaa
    16921 catatatatc tatgcttgtt gtttcaatta catccataag aacatcttta cattcaagct
    16981 caaaatcttc cattccttct aaatattcat catcttcatc aaaaaagata taagaattat
    17041 atagccattc ttcaaatttt tcttcaagta tatcccaata cgaactccaa aataatccac
    17101 aacctgatat ataagatgct ctactttgat ttatataaac ttcatttaga agcttttgta
    17161 atctactgct ataaagaaaa tctgctcttc cactgcttga tattaattca tttaaatctt
    17221 ctattgacat taattcaact ttaccacttt tatcataagg agtataattt tcatcaaccc
    17281 atttatgtgg tttgttttct ctgcatactg ttgctatcaa ctctacaact tttgttctta
    17341 tcattttttt atcctaactt atttattttt tataaaataa tcatgatttt ataatttatc
    17401 tatttcttgt aatgctaatt taacagtttc atatctatca gcaccattta ttactacatc
    17461 accttttaca agtttattag cttctccacc tacaacataa acctttttac ctaaaccttg
    17521 cttataacta gacgcttcaa ttattaaaga atctttcaat tgccaatgta aaatctgagc
    17581 agccacttta tcagcatcac ctgtataaac gattacattt tcatacactt tattttcccc
    17641 tcccccttga gaatttgata tagatttgtt tagtatacct tctgctatca atttagcaac
    17701 tttatcttta ttattaacat aataatctgt atctgcttta ctatctacaa aacatacttc
    17761 tattaatatt gctggtgcaa tagtatgatt aagccaataa agacctctaa catcagactt
    17821 tgcaccccta tctttgaata ctgtagctaa cctagtattc actctttctg cataggtttt
    17881 gccattatta gtcttatata ttgtttctgt ccctactgga gttgaagttg tagcatttga
    17941 attaaaatga atctgaacag ccaaatcagt ttcctgttta tttgctatag cacattggtc
    18001 tgctaaatga ttacttgatt catcaactct tcctgtataa acagtagctc cacctatttt
    18061 aagccattta acaattaagt cattcagtat tcttgtttct ttgctttcgt ttatataccc
    18121 aacagctcct gtaccttttc caattaaagt gtgccctggt attattacta ctttcaaact
    18181 aacatctcct ttacaattaa atttaacttc tatttaatat atgcataacc tagaattttg
    18241 tttcttttta tatcttatac ttttattata acccattttg taaaaatata gaatattaag
    18301 taaataatgt taaaattata attttgtata taagacatta cttgattgca ataattttat
    18361 agcatattca tctattctat atccagttct ataatatata tgtttaattc cagcttgaat
    18421 tatagcctta gtacaattta agcatggaaa gtgagttaca taaatagagc aatttttaac
    18481 tgaaatacct tcttttgcac aataataaag ggcattttgt tcagcgtgaa tggttctaat
    18541 acaatgtcca tctctcattt catgaccaac ttcactacaa tgcttatctc cagaaatact
    18601 accattatat ccagtagaca ctattctgtt ttctgaattg acaattatag ctcctacata
    18661 agctctatca caagttcctc tttcagctac tgtttcgcac agtctcataa aatattcttg
    18721 ccatgagcat ctattttcaa gttttttatt caaatcatat acctccaaaa tttatatcta
    18781 atatataaga tttatacctt aaaaataata ttaaaaacaa atttatatga taaataaaat
    18841 ttattaatac tgtttttagt ctaaattatt tcattttaaa ttaaagttac aaggaatcta
    18901 agattatata tatctttctt cgtaatatga atgttacaat ataaaaaact gtctcaataa
    18961 aatagagaca gttttacagt caaagtttat aatacaagtt catatttttc tttaaattct
    19021 tttacagctt cgggccatgc cttttgttct gtaatacctt tttctttagc tattttaatt
    19081 gcaatctctc ttatcattgc agcgccttct aatattccca tttttaatct cccttcaaat
    19141 atctttttgt atagaatata aatagtgcta agatttatta catgctgcat atacaatatc
    19201 tatacaacac ataacttgta aattcctttt attttcatac gtctttaacc gacatataat
    19261 tatattcaat aagttttaaa attatgaact caattataat tatttttatt tttttagaat
    19321 ctaaacacta aaaattgtag gtaaatttgt cttatgcctt tataaatatt tctacagttt
    19381 taattactgg acatatctat ttttattata aaaagcgatt aaaaatggag aatatcctca
    19441 taaaaactat atataagcaa aacggttaaa ggaaaaagaa gaaatttact tgaatgttaa
    19501 aatgcgttgc ttgaagcaat gggcagtttt gcctataata tcggtaacaa ctgaaagaaa
    19561 ggtggttagc cctaatgtta agcgaaaaca ttaaaacaat caggaagtca aaaggacttt
    19621 cacaacaaga acttgctata aaactgaatg tagtgcgaca aacaatatct aaatgggaac
    19681 aaggactgtc ggttcccgat tctgatatgt tgatttccat atcagaggtg cttgaaacgc
    19741 ccgtaagcac tttacttgga gaaactattg ttgagtcgga agttgataac ttaaaagtga
    19801 tttctgaaaa attagagatt ataaacttgc aacttgcaca aagaaaaaac atgagaagaa
    19861 aaatgtttca ttggttattt attttattat gtgcagtcat attaatagtt tttgtggtct
    19921 tagggagact aaatagtcct tacttggatt ggaattatac cgaccctgaa accgctgttt
    19981 ttggagtagc ttttcatgct tttgagtggc tatttgtcag attagtaccg attgtcctta
    20041 taggagcaat tattggaatc tttttgacac gtaagaaaat atgaagtaac tctgcttttt
    20101 ataatacagc aagcgttcct taaaagcgtc attctacatg gcaatcgagg acgactatgt
    20161 aaggaagaac ctttttgatt tccaactaag cgaagttatt gaggacgaca gtaggcccaa
    20221 aaccgcatta agtaaagtac aggaagcaag actgctggac ttatactctc atgcgtcaga
    20281 tgcaggaaat gcgaggtctg gtagcatgat ttacaacgat ttgtacaacg actacgagta
    20341 gaaatatatg gagataagta ttatctacga aatcagaaaa gaaatgactt gataatttac
    20401 tcatgttata attattgatg tgaaattaat aaatcgaaat tccttttttg gatttaacaa
    20461 tactgagtaa ggaaaaagtg aattactttt gcaaggaggt gttatttgga tggtgctagc
    20521 aggtattttg attgtagtac aaggcttttt gactggtcct ttagcaaaaa aaattggtga
    20581 gaagcgacta atactgatag gtatgttttg caaagcctta gtagcgtttg ccatggtatt
    20641 gacaactggg tttgttagtg tgttggtggt ttttggctta tttgcggtat cttctgctat
    20701 tacagtaccc acattaaacg caaccctttc aaaaactgac aatcagcata aaggaacact
    20761 gatgggattt gccagtaccg ccggaaatct tagcaaagtt attgctcctt tattaactgg
    20821 gtatctgtat gaaagcaata ttgaattacc atatataacc acgggaggga tagccttaat
    20881 cggagtggtt atctgctata tctggataaa aaatcaaaag aacaaatgat ggaagaggac
    20941 gtttatatgg aggttataca cagtaactaa tctggtatag aaattcaatt gaggcttttc
    21001 tgctttatta aagtcttgct actacttaac cctataatat ccttaattca ttatgacaaa
    21061 ttccaattca acagttttga tacacataag gaggtaacaa tactatggaa aattatcaat
    21121 gaagtgctaa ttctttatca gtgaagttat ccataagttt ttctacctgt aatcgtctgg
    21181 tgctttttgc cagatcattg gtaggtaaga aaaattcatc aacagacaca tgaagtaatg
    21241 atacaaggtc acttgtatgc ttggatgttg ccctttattt tcaatattag ttaagtaacg
    21301 tgggtcaata tttgtaattc gattttaggg aaatatttaa agaaatattt tcgttttatt
    21361 tctttaaatc ttcaagaata ttgatagttt tttgaaagta ggtgtgtatt atgaagaatg
    21421 agaaaaaaat tacagtcgca ttgctcattg tttatttatt gattttatca tggattattt
    21481 tatttaagct acaattttca ttttctgcgt tagaccacat aagacaaata aacttaattc
    21541 cttttggtgg ttcagttatt gcaaatggaa aaattgactt cgatgaaatt ataaacaatg
    21601 ccattgtttt tatccctgta ggtgcatatt tcagtttgct ttttaaaaat aaatctgttc
    21661 taaaagcaat aggttctgtt ttaggaatta gctttgtata tgagataatt caatttatat
    21721 ttgctatcgg agcaagtgat attacagact taattagcaa cacattaggg ggtataattg
    21781 gaattgcatt agtatatgta ttatctattg ttctaaagga taaaacacat aagatattaa
    21841 atcgaatagc aatagtatgt actgtattag tagttgcctt cttatttatg cttcttgctg
    21901 taaataatat attttaatgc catgttaatt acatgttgtt ttggaaaaga ctttaatctg
    21961 tcaaagtttg cttttgaaat tgaacatgaa attacaccaa tggaacaaat gaaagctata
    22021 aatttttcat tggagaaaga tgactgtaca agtaaagtat taatatacaa tccagacaaa
    22081 tggaaatatg tagaatacta tttctataaa gatagaccta ttaaaaaaac tattggaaaa
    22141 atttattcaa ttttgcattt gtcacaataa aatatgtttt gaaatattga atacttaaat
    22201 atgcaaaaaa acaaatgtaa ctggctatgg ggctatatct actaaaaagc tatgtgatag
    22261 gttgggatta aatttaaaca tcacttctag aaggcatgaa tacacaaggc tgacaaacta
    22321 tacagttatg attaaacgat accaacggga ttgaaaattt cgtgaagaac tttgagaagt
    22381 cctattacac atgagcaaca gcaacgaagc tattgaagca aggacgcagg caatctagcg
    22441 gttatctgac aaaggagttg taggacttga atgtagatac tatcagaacg gatataccga
    22501 ccagctctgc ggttacagat gtgattgtgt agaatatcga gcagtcggga acggacactt
    22561 tatctgctac ctacgaagta gatcagcaga taaaagagag agaacagaca acagcggtca
    22621 aggcaaccta taccgtaaaa gtccatgtgg acgctgacgg ggatatggta atcgttcaga
    22681 accttactct tgcaccagca atcgaaaaat cagactatga gccaaagata tcagaaacag
    22741 acgcaagcgt ggacgctgat accgttaatg acgctaccgc atttctggaa acattctttg
    22801 aggtgtaccc gacagccaca gaaaaagaac ttgcctatta tgtatcggaa aatgtgattg
    22861 aacctatcgg cagggactac ctttattctg aattggtaaa cccagtcttt acaaaggacg
    22921 gggacaatgt gaaagtcaag gtagctgtga aattccttga taatcagaca aaggcaacgc
    22981 aggtgtcgca gtatgatctt gtactccaaa aggatagcaa ctggaagatt ttaggataac
    23041 atggaaatgg gcttgtatca taattatagt gtgcaagccc ttttcatatt gaagtatcaa
    23101 taatcaataa ttataattag gtacatgaat ggagggataa aatgttcaaa attggtgagt
    23161 tttcaaaatt gacacaagtt tctactcgta tgttgcgtta ttatgatgaa acgggtttat
    23221 taaaacctgc taaaattgat tcattaactg gatatcgtat gtattcagca gaacaaatct
    23281 caactttaaa tagaattata tatttacgtg atagtggctt caatgtcgct gaaattgcat
    23341 tagccctcaa tttcagtaat aatgccatta tagaacagct tgatagaaag tctattgaaa
    23401 ttatggataa tatacaaaaa gaacaagaaa aactaaagaa aattgagatt gctaaaaaag
    23461 aagtgttaca tggcaaaagt gaattgtatt ttaacataac cattaaagag ataccgagtt
    23521 attgcgtttt atctttaaga aaagaaatac caaattatta ttatgagggc gaactctgga
    23581 aagaactctc tgcttttgct aaagaaaata aaataaatat ttctgatgaa acattctcaa
    23641 tatatcacaa tgaagaatat aaagaaaaag atgttgatgt agaattatgt gccattgtaa
    23701 ataaaatagg aaaagctaca ggagatttta agtttcgttt taccgaacca gtttctatga
    23761 tggcttgtac tatggtatat ggagattttt ctaatatttc ttatgcttat cgttcatttg
    23821 caaaatggct tcaagaaaac agtcaatata agatgaaagg ttcaagccgt caaatagttc
    23881 atcgtggacc gtggaacgag aaaaatccag agaactatct aacagaaatc caaattcctt
    23941 tagaaaatct cttgtaaaaa atgataatct gccttgactt taacatggtg ttaaggttta
    24001 tcgtttattt atcaagaaga aaacggagga aaatataatg acaacttata aagtaaattc
    24061 aataggtact atctgcaaca atgaaaaagg tgctttcatt caactagaag caccatatat
    24121 tctagcatta agggcattag acggattcag tcatataaat gttttatggt ggttcagtga
    24181 ttttgataat acgaatttta gaagtgtatt ggaaacagaa cagccttata aaaatgctcc
    24241 agagaaaatg gggatttttg caacaaggtc gccagtacgc ccaaatccga ttgctttatc
    24301 agcagtagaa attatcgata ttgattatat gagtgggctg atacaaattg cttatattga
    24361 cgctaatgac aaaacgccta tcttggatat taaaccatac acgccgagtt tggatagagt
    24421 ggaaaatccg caagttccag attggtgtag tcattggcct cacagcttag aagaatctgc
    24481 ttcttttgca tgggaaagcg aattcaattt ttaaggagtg atgaaccatg agtttgcagg
    24541 actacatttt tgaaaaacca gtaattgaaa cagaacatct tattttaaga acaatgaata
    24601 aaaatgacat agatgatttg aaagaatgga ttagtgatat ttctttatac gagtattggg
    24661 ggaaacgtcc ctcaaaaagc gaaaaaaatc cagaattact ctttgataag tcaaaaaaat
    24721 ctactaaaag ttttcattgg ggtattgtcc ataaacaaga tagcaaagtg attggtgaaa
    24781 tatggatata tcttattgaa aataatagaa tggcaaaagt ggctttcaga ctttctaagc
    24841 attatcaagg taatggacta atgagtgaag tgttgaaaaa cgtaattgaa ttttgttttt
    24901 caaaaacaga attacaaaaa atatggacag atgtgcatat tttaaatcat gcttcttaca
    24961 agacattgga gaaagtcggt tttcaaaggg aaggacatat tcgagggggg aaaatggtaa
    25021 atacctattg cgactactac ctatatggaa ttttaaaatc tgattatatc actaggtggt
    25081 gctatggcta atattgaagc gacgattggc gaagcaatga acaatacagt tccaaaggtc
    25141 tatgatacga aaagcattaa aagatatgtg atgaatttat tagatatggc tcattaaaaa
    25201 gtaccaaaga aataaaagtt accatataga caaaattggt tttttatatc ttttgatttc
    25261 ttataagata atggcattgt gcgacaagct atgcaaaaaa ttttcatgta tactttgatt
    25321 ataatttata caaggaggta ctgatatgta cgcatatgaa aatattgaaa tgtcattaaa
    25381 ctatattgaa caacatttaa gtgagaaaat cgaaacagag aaactggcgg aaattgcctg
    25441 tctttctaca ttttattacc aacgcttgtt taaaaggtta gttaagaaat cagttcaaga
    25501 gtatatcaaa cttcgccgtt tagcaatggt aatagcagaa ttgaataatt cagaggaaag
    25561 aatattagac atagctttaa aatatggatt ttcagaccat gctaacttca caagagcatt
    25621 taaagaagtt taccatatta ctcccgatga atacagaaag aatgttccta tgttaaatac
    25681 atttgacaag ccagaacttt cttcacgtta tatcatgatt gatgaaaatg tcccattgat
    25741 tgtaggaaat attgttttgg aaattcaaag aaaaacatta agaacaaagg aaacatattt
    25801 tggttttgaa aaaacagtaa atatttctga acaaattcct gctggagaaa gtacaggaat
    25861 tgatgttcca ggtcagctat ggcgcgaatt ccatgcaaag aaactgcaaa ccgaaaatgc
    25921 catttttaac aatgatattg aacttggtgt atctcactta gcaagtccag ataaaggaac
    25981 attcctttac tttgcaggag gtatggtttc atcaacagat gtatgtccta aaaatatggt
    26041 acaacataca ttaccagcag gtgagtatat tgtatgcaaa attgaggcag aatcttttga
    26101 agaacttgtt actactgctt taaatcaagc aaataagtat ctgtttgaaa cttggcttcc
    26161 aaatcataat ttggttacaa agccattcat ggcagaaaaa tattacaaag gaaatgcaga
    26221 gattaattat atggaaatat ggataattcc tgtgtaaaca gtaaatgcta aataggaatt
    26281 atctgacgta ataaaaagga aatagaaagc ttataaagga gggcatagtc cgttgtgata
    26341 aacataaaag atataaatta tgtacctaat attagtgaaa taagcgttta tattgaaaac
    26401 tctgcatttg atgagttgta tcagcatatg aacaatgaat ataaagcagt ttgcaaaatc
    26461 gaatacagta aagatgtctg ggcacgaggt tggaacgtaa agtttaaaaa atctgggaaa
    26521 tcattgtgtg ttgtctatcc gaagaagaac tactttactg tattggtggt tgtaggtaat
    26581 aaagagaaag aagatgttga aaacttgtta cctaggcttt ctgaatatgt acaggaaatt
    26641 tatcgtaaca ctagagaggg aaatggacaa aggtggctta tgatagactt aaaatctaaa
    26701 aataatgtat atcaagatgt attaaggctt attgaaattc gttatgccat aaaatgtgta
    26761 aaaccgtaat aaaaatattt ttcacatacg taatctcaag gtattatcgt tgagacaagt
    26821 aacctatata attatgggaa atgagatttt tagatactta agaaagtact ttagggggct
    26881 tgcgttatgg aaagatttaa gtacaattcc acacaatcaa atacaatcta ataaaacttt
    26941 actaaaaatt gaatgcaata cagctttaaa ggctcgtaca aactatatgt tttgtgcggg
    27001 cttttttcat ttaataggaa tttggattcg tcagagagta tattataaaa ataaaacgat
    27061 aagggtacct taaaaataag gtacctcagg ctgtagacaa agtcccggac tctcgtctgg
    27121 gcttttctta tatttgagtt atccacaatt caacttttta agagttatct tattaagaaa
    27181 aaagtgtatg ttttccaccc tatagtttta cttctgtgat atttgcagat gccactcaag
    27241 tagaaagtgg tggtacggca gaagatgtca tgcagagttc agaaagtctg ggactaccgc
    27301 ctaattcctt agacacggaa agctcaatca agcaggggtg taagtatttt gcgtccctgc
    27361 tttcttcctg caaaaatcaa ggtatcgacg atttgaatgt agcgatacaa tcttacaact
    27421 atggcggggg ttatgtgggc tatgtggcag gaaaagggaa aaagcacact tttaatcttg
    27481 cagagagctt cgcccgtgag aaatcgggtg gaaagaaagt aacctacgcc aacccgatag
    27541 ccgttgcaaa gaacgggggc tggcggtatg gctatggaaa tatgttttat gtggaattag
    27601 tcaatcagta tttaactgtt ccgcaggtat cgggagagct ggcacaaaag gtaatgaatg
    27661 aagcgttgaa gtatcaaggg tggaaatatg tgtttggggg aagcaatccc aacacttcct
    27721 ttgattgtag cggacttgtg caatggtgct atggaaaagc tggtatctac ttaccgagaa
    27781 cggcacaaac acagtatgac gcaacccagc atatcccttt atcgcaggca aaagctgggg
    27841 agttgttctt tttccattc
//
LOCUS       DAEPIN010000055        26523 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.055, whole genome shotgun sequence.
ACCESSION   DAEPIN010000055 DAEPIN010000000
VERSION     DAEPIN010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 26523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26523
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.055"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            <1..939
                     /locus_tag="KR250_002607"
     CDS             <1..939
                     /locus_tag="KR250_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBH0068529.1"
                     /translation="LYKHISSLCKKIKLGINQSPIMAQDIKIKYPFAFELANIAKKTI
                     EKDLKIEINEDEVANIALHIGGAVERASYNNKDKVFKTIIVCTSGIGTSMLIKAKLEN
                     IFKEKLEILKIIPSYLVEYIKVIDVDFVISTVPIEVGDVPVINISPILSEKEIRLIEK
                     YIETGNIYLDLDIKSLFDSELFFTDLVFKRKEEVIDFMASKLVQKGYIDEDMKKSYFE
                     REKIATTEIGNMVTIPHGAIGKIFDNKIAIGILKKPISWEISDVRLIIMLALDKDKIL
                     DYEVIFSKIYKRVDSIAKVISICENSSFEKFVNLFN"
     gene            1106..2023
                     /gene="pfkB"
                     /locus_tag="KR250_002608"
     CDS             1106..2023
                     /gene="pfkB"
                     /locus_tag="KR250_002608"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HBH0068530.1"
                     /translation="MIYTVTLNPSIDYIVKLDELKTGSTNRVKEEYVYPGGKGINVSR
                     ILKELGNDNTSLGFISGFTGEYIIRTLEEKELKTDFIKIKSGFSRINVKIKALKETEI
                     NGQGPNIDDEDIDTLYKKLDKLNQDDILILAGSIPSTLDEKLYENIMARLEKKNIKVV
                     VDATKNLLLNVLKYKPFLIKPNNDELEELFGVKLNSIEDMVKYARRLKEMGAINVLVS
                     MGKDGALLITEEDEVLISDVPKGKVKNSVGAGDSMVAGFISGYLNTGKYDYALKLGAA
                     SGSATAFSYDLAKREYIDKLVNEISVKKF"
     gene            2060..3979
                     /locus_tag="KR250_002609"
     CDS             2060..3979
                     /locus_tag="KR250_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBH0068531.1"
                     /translation="MKIIDLLDEKSIKLNLNSKTKSEAIEELVDLVANSGNLNDKENY
                     KKAILAREEMSTTGIGEGVAIPHAKNSSVTKACIAAAVSKEGIDYESFDGSLSNLFFM
                     IAAPDGANNTHLEVLSRLSTILMDEDFRNKLINSSSEKEFKEIIDKKEREKFSEEYQD
                     EKVAEKNIIDTKENDANKYPKVLAVTACPTGIAHTFMAAESLNKMAENKGVSIKVETN
                     GSAGVKNKLTKDEIENATCIIVAADKNVEMARFNGKKVIKTKVADGIHKAEELIDKAV
                     NGDAPIYHGGDGSHNESNEESESGFRKVYKHLMNGVSNMLPFVIGGGILIAIAFLLDD
                     YTINPSNFGSNTPIAAFFKGIGDKAFGFMLPVLAGYIAYSISDRPAFVVGFVGGALAG
                     DGGSGFLGALLAGFIAGYLVEGLKKIFSVLPASLEGIKPVLLYPLLGTLLMGIIMTFL
                     VIPPVTAINNAMVGFLNGLGGTSKIVLGLVLGGMMAVDMGGPVNKAAYVFGVASLESG
                     QFEIMAAVMAGGMVPPLAIALATTFFKNRFTKEERDSGKVNYVMGLSFVTEGAIPFAA
                     GDPLHVIPACVGGSAVAGALSMLFNAALRAPHGGVFVIPVVTHPFAYILAIAVGALVG
                     MMLLALLKKPLNQEV"
     gene            4475..5482
                     /locus_tag="KR250_002610"
     CDS             4475..5482
                     /locus_tag="KR250_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBH0068532.1"
                     /translation="MIFTIIFYKKGRLCLLNLQSKWVNFLKPTYVSDEIKRKKLQVII
                     FFSITYGLSYSLGLLAICFNHLIDSEILAQFMMILPLSSVSIAKFYTEGRNNDKYNFY
                     SSVILFFIIYFFIFIIESLKLISINQFQLMYLMLILISSLCVIVYSYKITSLYIFKNF
                     KIGILLFFYFLFSQIIFGLIISDNQFDYKNFLNYILLPIISLVYIYPFLCEEYGWRSF
                     LQSILFDRFGKKIGIVMVGTCWSLWHLPLQFTLYSPQTPIIGAIANLIYGIGLSIFLG
                     YVYMKTKNIWFCSIMHVLINSLGLIFNDSEIIINYHSIIQRLIFILLFYTPFLFTKEY
                     K"
     gene            5862..7388
                     /locus_tag="KR250_002611"
     CDS             5862..7388
                     /locus_tag="KR250_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBH0068533.1"
                     /translation="MNESYWFLNSSPKEYNKLGENIKTDCLIVGGGITGLTTAYLLAK
                     EGKKVVLVEADKIGYGTSGRNTGKVTCQHDIFYSKIEKKYGLDKAKSYYNANNEALNL
                     VEQIIEENNIKCDFKRETSFVFTEKEDTIKDIKDEYRTCKKIGINCEYHETIENIPLD
                     IKGAISFTNQGQFNPKKYIDGLAKAAVNLGLKIYENTPVVDLEKGKICRVKTREDNII
                     EAENVIISSHSPWYDGLNFYFAKEYAERAYLMVAVLENKLADGMFISIDDPSITFRQY
                     NDGSENLLIFGGGDHKVGQGGTEKEIFDDLEHYGKEVFKVKDFKGKWSAQDNMSFDNV
                     PYIGYINKREDNIYVATGFSKWGITNGTAAGIIIKDLIINNNSDYKDTFNPSRLGSYF
                     SKDFIKENANVAINYVSGKLKIGSGDMPKNNGEGKIVNIDGKRYGVYKDDNGDFYIVD
                     TTCTHLGCELNFNSEEKTWDCPCHGSRFDYKGNILEGPALKPLKLYGHGDNDVNPKLL
                     "
     gene            7416..7973
                     /locus_tag="KR250_002612"
     CDS             7416..7973
                     /locus_tag="KR250_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0068534.1"
                     /translation="MNKLLKPITILLYIVYILSAVFFVANKEYGSLGLVAISLVGTFI
                     LSFINKQTERLLSKELYIMLALFIMFSSLLGTCFNFYDINYYDDFLHLWSGIISCIVA
                     FSIIKYFYTQREISKMSKVFLVIFLFMFSMGVASLWEIGEFLMDTFVGTTTQAGGLED
                     TMIDMVDALIGTIITIPFIVKKSNI"
     gene            8495..9538
                     /locus_tag="KR250_002613"
     CDS             8495..9538
                     /locus_tag="KR250_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0068535.1"
                     /translation="MLKKWFGIVKKRQKSESAKEEGEVILKNEKILSEEKLIDEEGVV
                     VNIDNEILTKVEVVNDDNEIEEKIIEEDWLISENTIKLDDKEAIINDKNIELCKKEVQ
                     VESEKIGLNKFEGLDQNENYNLEKNVIEEKKVSECLTEEDLEYIKEIKIKRGKSIKAI
                     NLYTKEEWVFDTHIQCSKKLKVPLGYIRENLKYGYMDYFGDAINYLSEVLNIDEYCKS
                     EWSYLDNSKSPSEIFNILNNKIFSIRLSNEKRNEILTNDKIEALKMNYRFECIDEEYD
                     EYFKKYKSIIKRGGKKKVELVNKKGDILEIFKSLEECAIYLQKEKNEVIQMLKYGDTK
                     VGRNFIRYSLRSI"
     gene            9649..10791
                     /locus_tag="KR250_002614"
     CDS             9649..10791
                     /locus_tag="KR250_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBH0068536.1"
                     /translation="MSEEKNPKENTLDESKNSDNVNSKKDDVSDEKNTDNKVKKREIK
                     LNNKTLKAIVAIVAVGAICLGGGYVFGKEAGRKLPATSKHYNSSEVVATIGDKKITGE
                     MLRKRMEPLFYLNAKTTMTDDEIRSYESNMIETMITSDILSSEAAKEKISITNDQLDA
                     QYNNLMSSIESAMGMTKEEFLKQFDLTEKYIKDDMKKELVASKYLNEKSKVTDKEAEN
                     YYNKNKSNYLQVRASHILIKTVDDKGKQVSSSKKAELKKEAEEILKKAQAGEDFATLA
                     KKYSEDSSAESGGDLGFFGKGQMVESFEKAAFALKKGEVSNKLVESDYGYHIIKKTDE
                     KYQPFEEVKSDLVSSLTSEKQNLLIQNLKEKYNVKITDKYQMKAKE"
     gene            11245..12636
                     /locus_tag="KR250_002615"
     CDS             11245..12636
                     /locus_tag="KR250_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HBH0068537.1"
                     /translation="MSSTESVSKKSGHPKSFWLICMTILWERFSYYGLTALLVLFFTA
                     SVAQGGLGLSTQEATSMFGLFTGLIYLTPLVGGWLADKVLGQQKCISIGALLIAIGDF
                     VLFASSTSIAMVWVGLSIIILGNGFFKSSCSNIVGDIYPKTDVSRKDAAYSLFYMAVN
                     VGAFIAPIICGLLSDNVFAVRGASGDIVSYGYKMAFLICGIGMMLGMIIFTLLAPKLL
                     NEVGKYPVTKGNKGEKIEYGPLTKLEKNRIIAMVILFLFVVLFWTAYYQTQSSFMIYV
                     RDNVNRTLFGFEMPVAWLTSLNAILCVALCPVLATLWVKLSHTKRGDFSIPVKMGLGM
                     IIMGVGFIVMVFATLANGPDGMVKASILFIVLTYIFTTVGELFLSPIGLAMFNKLAPA
                     KYATLSMGAWYLTSFFASLLSGKIAGFTATLGFLQIFGGIATVLIIFGLILLGIRNVL
                     TRLMAMDLLTKEK"
     gene            12889..14682
                     /locus_tag="KR250_002616"
     CDS             12889..14682
                     /locus_tag="KR250_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBH0068538.1"
                     /translation="MNIKDRLSGLRKFMEEKNIDAYMIPSSDNHQSEYVGDYFKSREF
                     ISGFNGSAGTVIVTKDEAGLWTDGRYFIQAESQLEGSTIKLFKMGQEGCPTTDEYLYK
                     NIPEGGTLGFDGRVISAREGATLAEKLSKKGIKIEYQYDLIDSIWPDRPALSDSKAFL
                     LDVKYCGESFSSKLARLREKMSEKGTSTHVITTLDDIAWLFNIRGGDVKYNPVVLSYA
                     VITLKEVYLFVDESKLNEEILNELAKENVQIKPYNDVYEFVKNIDKTEKVLLDGTKLS
                     YTIYNNIPCEVEKVDEFNPVMFFKAQKNEVELENIRNSHVKDGVAFTKFMYWLKKNVG
                     KMEITEISATQKLEDLRREQEGFFEPSFNTIAAYKEHAAMMHYSATPESNYKLEAEGL
                     FLVDSGGQYYDGTTDITRTTVLGPISDELKLHFTSVARGMINLSKAKFLHGCRGYNLD
                     ILSRSCMWNMGIDYQCGTGHGIGFVLNVHEAPNGFRWRVVPERFDSAVLEEGMVTTNE
                     PGIYIEGSHGIRTENEIVVRKAEKNFYGQFMEFEVVTLAPIDLDGIVPELMNKDEKDY
                     LNWYHKLVYDKISPFLTDEEREWLKVYTRAI"
     gene            complement(14763..16145)
                     /locus_tag="KR250_002617"
     CDS             complement(14763..16145)
                     /locus_tag="KR250_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HBH0068539.1"
                     /translation="MSVLKTQEKLKQPRDLYFCCSIFTFVGLAYYGMKLVLLLFLAEQ
                     VSKGGLGLTPAESASMLASFLAWTYFSPVIGGWVSDRFLGPKNCIILGMLMVSIGYFL
                     GYIANSKMDINIMIFLSGLGIGFYKGNLHTMVGNLYTNDDPRKDGAFSITYMATNLGT
                     LFGPLICGLVANEWFAMKSGLSISMYGYRYVFLFSSITVLIGLFFFIFGNRKFYKPLN
                     NDPSNGRESIRKSLEYKKMLASRPLTLIEKKRIIVILVLAFFTVFFWIAYNQASMSIA
                     LYTKEHINLTVGNFQIPTSWIDSYNGLLCVILGPISALVWVKLSQTKKGDLSVPKKMS
                     LGFILLAIAFLFMIVAVIQTGTDPNSIHKASVFWVVGFLTFQSIGEICFSPVGYGMVN
                     KLSPEKYISFLMGVWFLGKFAANKLSGYTQAIIDQLGMLQVFIVIPSFLLIFGIILLI
                     MNKKLVELSN"
     gene            16403..16750
                     /locus_tag="KR250_002618"
     CDS             16403..16750
                     /locus_tag="KR250_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HBH0068540.1"
                     /translation="MIRKKSELQVEVKENLNGGIGKVKLENIIQNEELKGKGRLFKRV
                     ALPVGSSIGVHDHTTDFEVYYILKGKGKVFDNGEFVEVNEGDVVYTADGEKHSIENIG
                     EEDLEFVALVLYM"
     gene            16768..17802
                     /locus_tag="KR250_002619"
     CDS             16768..17802
                     /locus_tag="KR250_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0068541.1"
                     /translation="MTGKIEEIKDKINRFCNSNLNQEYKDISFKILQNLLDNNKEIIH
                     SSRADIWSSAILNIVLEQNLLYNKKHPLHITKKEFSKQIGVSLNTINNKSSLIKDVCS
                     IDFLNQDTIAISNVEFWVKETNSKSKSVDLDLEKKKILYKRYIKLSQDANSDIESIRY
                     MEEAVNIGKSMITKEDKELGFWKGLSTRAYMIALESLARKLESINNLKESKKVLEYLI
                     EINPSDEQGIRYKLLNVLIRLNDRTSINKLFELYKEEKSATWMYSKALYYFKNKSMFL
                     ANDSIKVARSKNKYVGLYLLDWRNAFGREFVTEEEKAEAVYYYDENIVIWNEVKGSMD
                     WLLKKMLEFS"
     gene            complement(17851..18900)
                     /locus_tag="KR250_002620"
     CDS             complement(17851..18900)
                     /locus_tag="KR250_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBH0068542.1"
                     /translation="MEINYKKRNFKSFAKRKNIEISVQRYLIDALSYMALGLFSSLLI
                     GTIFNTLGDNFNISLFTEVISPLAKQVTGPAIAVAIAYGLQAPPLVLFSCALVGACGN
                     ELGGPVGAFVATLFAVEVGKLVSKETKIDILVTPAVTILIGVLVAELIGPAVSAFMTS
                     FGNIIMQATTMQPIIMGALVSALVGIALTLPISSAAICMMLSLGGLAGGAATVGCCCQ
                     MVGFAVMSFRENGVGGLVAQGLGTSMLQVPNIIKNWKIWIPPTVASILLGPLSTTIFK
                     MENIPIGSGMGTCGLVGQFGTVTAMQSAGKGGISMWVGILLLNFILPAIVTLLISEFM
                     RKKGLIKPEDLKLDV"
     gene            19175..20710
                     /locus_tag="KR250_002621"
     CDS             19175..20710
                     /locus_tag="KR250_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0068543.1"
                     /translation="MIFVKNKKYRKIAIIISIPILLVAQYYLFKFGILRPMVKGVEIE
                     IVDGEYVKDIDKYVVKLNETVTLSTGEYIKVPSYAKNPNIWFNVLDNSGTLKIDGNKM
                     TALKVGYSSVGIMKNSRVLKKATIKVVDPEVESLDMEINGDLKHVGDSATIESTIEVD
                     YDKFKKSYKPVYKSSNENILKIKGNKVSAVGVGKATIYAKCGNKEIERTFRIQARVAE
                     INMDDIKIEVDQNEKLKPDIITSPPGLEPPKIYYELVDSKLPVERAISLSSNGNVVGL
                     REGKDRVRITCGDKSKVITVTVVKETITNDYIQNLVGSHKIVNNKVIIKLEWDYMKDV
                     FDYDIYLKDKLSGESFKKIKSITINESDLGKSNKVYATIEVDMKGNQNIDFDIYVIGK
                     KDSDTTKPSNVINITNGNGNSNNDNNIKDMRVENISANVDRDNNVIRVNWSSISHKDC
                     TYSIYVKNNTNGDGEFTLYQSDIQGNEHTISIPEGDLNLDIYVVANYNGNSSQNSDVI
                     NVR"
     gene            complement(20895..21581)
                     /locus_tag="KR250_002622"
     CDS             complement(20895..21581)
                     /locus_tag="KR250_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HBH0068544.1"
                     /translation="MKTIGLIGGMSWESTITYYQVINTVIKERLGGLHSSKCILYSVD
                     FQEIEECQSSGNWEKSAKILADAAIKLQEAGADFIVICTNTMHKVSDKIQESIHIPLL
                     HIADVTATVLREKEIKKVALLGTKYTMEQDFYKNVIINNGIEVLIPNEEDRIIVNDTI
                     FNELCLGIISESSKKAFLSIIDKLGKQGAEAVILGCTEIGLLIKQNDTSIPLFDTTVI
                     HAIEAALSSI"
     gene            21706..23088
                     /locus_tag="KR250_002623"
     CDS             21706..23088
                     /locus_tag="KR250_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBH0068545.1"
                     /translation="MPINSFENYPMTWKPKLDNRKPPIYKTLAMLLEEDIKRGNLNPG
                     DKLPPQRELADFLDLNLSTITRAFKLCEEKGLICAKVGKGTFISSDVNVSNTLLYQTE
                     SKDIIELGTVHPPYEQNTYIIDFIKNVLKQPEMDRFLQYMSPSGTYMQKKSIAKWIER
                     NNVYTSEENILLSTGGQNAICATLLGLFKAGDRIATDSLSFSGIKSIAKMIGIQLVPI
                     PQENNEISIEYLESYCKNENIKGIYLIPDYHNPTTHTMSDLSRKKIAKIAKQYNIIII
                     EDAINSIFRDGIQTPIFSLASDNTIYIFSTSKFLCAGLRVAFVVAPKRYIENLENALY
                     NMNLMVSPFTAEIVHRLLYSPIIDKIIEEKRDAIIERNEVADKVLSDYNLIGDKNCSF
                     RWLLLPDELDGKSFEISAKNLGVQVYCAERFSVGNSTVPKAVRICVTAPKDVEELEKG
                     LNIIKSLLKS"
     gene            23490..24758
                     /gene="eam"
                     /locus_tag="KR250_002624"
     CDS             23490..24758
                     /gene="eam"
                     /locus_tag="KR250_002624"
                     /EC_number="5.4.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 2,3-aminomutase"
                     /protein_id="HBH0068546.1"
                     /translation="MNEQTRISLERAAELKSKIDDYIQARKTINRGLEKEEEINKRKQ
                     KILSILNGTEEDWNNYKWQLSNRITDVDTLSKIITLTKKEKEYIKEVGTQFRWAISPY
                     YLSLIDPEDICDPIKLLSIPTHIELEDEQEDLDPMGEEYTNPAGCITRRYPDRLIINV
                     TNECAMYCRHCQRRRNIGQQDSHKSKAIIQESIDYIRENEEIRDVLVTGGDALTLKDD
                     YLEWILSQLKEIPHVDYVRLGTRTLVTMPQRITDEFCNMLKKYHPVYINTHFNHPMEI
                     TKESKEACEKLANAGVPLGNQAVLLNGINNDKFVMRCLNQELLKIRVKPYYIFQSKHV
                     KGTKHFNTSVDDGLEIMEYLRGYTSGMAIPTYIVNAPKGGGKTPLLPQYLVSKGTDYV
                     MLRTWEGKVIKMEDEPAVDIKKLIKEQAQD"
     gene            complement(24851..25207)
                     /locus_tag="KR250_002625"
     CDS             complement(24851..25207)
                     /locus_tag="KR250_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0068547.1"
                     /translation="MKKALISLLVLSCLLLTAPVNESSYADSQKNVPVSQNTKLTQEQ
                     AKELLVKYNNEVDYIYQGNASDFEALKAKNLNGYVFLPDADGDIGYFVNENTSEIYYF
                     HPSGYLELVNQQQNNK"
     gene            complement(25358..26452)
                     /locus_tag="KR250_002626"
     CDS             complement(25358..26452)
                     /locus_tag="KR250_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBH0068548.1"
                     /translation="MQDKKIYLNIVLTVVVSYILIKVIDNYKYFFGVISLLMSLLTPF
                     IIAFVLAYIFNPLVKFLESKLNFKRIYSLLLTYGVLIVILISFILFTVPSIVNSLADL
                     VAQIPTYIDKTEQFLFDLGKSLQSVDPNTLKEYGDKIMSVMPKFSNLLIGSLGGIFST
                     TFSVGKFIVQFLLGFIICFYILLEKEKFLLFTKKVVYISLGKKYGDFIIELCQSLNLN
                     IGKYFTGKILDSFIVGVLSGIGLYFLKSEYALLFGTLMGVMNMVPYFGPVIGMAPVVI
                     INLFSNPTIALTSLIYLIIIQQVEVTFIEPKIVGGQLGLSPFFTILAVTVGGGFFGIP
                     GMILSAPVMGVIKIYFCEFVNRRHDKIQME"
BASE COUNT        10490 a         2908 c         4406 g         8719 t
ORIGIN      
        1 ttatataagc atattagtag tttatgtaaa aaaataaagt taggtataaa tcaatcacct
       61 ataatggctc aagatataaa aataaaatat ccatttgctt ttgaactagc taatatagct
      121 aaaaagacaa tagaaaaaga tttgaagata gaaattaatg aagatgaagt tgcaaacata
      181 gctctacata taggaggagc agttgaaaga gcatcttata ataataaaga taaggttttc
      241 aaaactataa tagtttgtac ttcaggcata ggcacatcaa tgttaataaa agctaaatta
      301 gagaatatat ttaaggaaaa attagaaata ctaaagatta ttccttcata cttagttgaa
      361 tatataaaag ttatagatgt tgattttgta atatctactg ttcctattga agtaggagat
      421 gtgccagtaa taaatatatc tccaatttta agtgagaaag aaattagatt aattgaaaaa
      481 tatatagaaa caggaaatat atacctagat ttagatataa aaagcctgtt tgatagtgaa
      541 ttgtttttta ctgatttggt gtttaaaagg aaagaagaag ttatagattt tatggcaagt
      601 aaacttgtac aaaagggcta tatagatgag gatatgaaaa aatcttactt tgagagagaa
      661 aaaatagcaa caacagaaat agggaatatg gtaactatac cacatggagc tattggaaaa
      721 atttttgata ataaaatagc aattggtata ttaaagaagc ctatttcttg ggaaatatca
      781 gatgttcgtt taataataat gttagccctt gataaagata aaatattaga ttatgaagta
      841 atattttcaa aaatatataa aagagtagat tctatagcaa aagttataag tatatgtgaa
      901 aatagtagtt ttgaaaaatt tgtaaattta tttaattaga tgggtagtat tgtatataca
      961 cctgtaaatt tggataatat aaaactaaat aagataaaaa cgataaattt ctcttttaat
     1021 taagagaaat tttttgactt tcaaggatat taatataaag ttaagatata aacataaagc
     1081 aaataaataa aaaagaggtg gcaatatgat ttatacagtg acattaaatc catcaataga
     1141 ttatatagtt aagctagatg agctaaagac aggaagtaca aatcgtgtaa aagaagaata
     1201 cgtttatcca ggaggaaaag gtataaatgt atctcgcata cttaaggaac ttggaaatga
     1261 taatacatct ttaggtttta taagtggttt tactggagaa tatataataa gaacattgga
     1321 agaaaaagaa ttgaagacag attttataaa gataaaaagt ggtttttcaa gaataaatgt
     1381 aaaaataaag gcgttaaagg aaactgagat taatggacaa ggtccaaata tagatgatga
     1441 agacattgat actttatata aaaaacttga taagctaaat caagatgata ttttaatatt
     1501 ggcaggaagt ataccatcta ctttagatga aaaactatac gaaaatataa tggctagatt
     1561 agaaaaaaag aatataaaag ttgtagtaga tgcaactaaa aatcttcttc ttaatgtttt
     1621 aaaatacaaa ccttttctaa ttaagcctaa taacgacgaa ttagaagaac tatttggtgt
     1681 taaattaaat tcaattgaag acatggtaaa atatgctaga aggttaaagg aaatgggagc
     1741 tataaatgtc ttggtatcaa tgggaaaaga tggagcattg ttgattacag aggaagatga
     1801 agtgcttata agtgatgttc caaaaggaaa agtaaaaaat tctgtaggag caggtgattc
     1861 aatggtagca ggatttatat ctgggtattt gaatactggg aaatatgatt atgctcttaa
     1921 attgggagca gcttcaggta gtgcaactgc tttttcatat gacttagcca aaagagaata
     1981 cattgataaa ttggtaaatg aaatatctgt aaagaagttt taaaatatta aaaaatatat
     2041 agaaaggtaa ttaatattta tgaaaattat agatttatta gatgaaaagt caattaagtt
     2101 aaatttaaat tcaaaaacca aatcagaagc aatagaagaa ttagtagatt tggtcgcaaa
     2161 tagtggaaat ttaaatgata aagaaaacta caaaaaagca attttggctc gtgaagaaat
     2221 gagtacaaca ggaattggag aaggtgtagc tatacctcat gctaaaaatt cttcagttac
     2281 taaagcttgt atagcagcag cagtgtcaaa agaaggtata gattatgaat cgtttgacgg
     2341 aagtttatca aatctatttt ttatgatagc tgcaccagac ggagctaaca atactcatct
     2401 tgaagtatta tcaagattat ctacaatact tatggatgaa gactttagaa ataaacttat
     2461 aaattcaagc tctgaaaaag aatttaagga aataatagat aaaaaggaaa gagaaaaatt
     2521 cagtgaagaa tatcaagatg agaaagtggc tgaaaaaaat ataatagata ctaaagagaa
     2581 tgatgctaat aagtatccta aggttttagc agtaacagca tgtccaactg gaatagcaca
     2641 tacatttatg gcagctgaaa gtttaaataa aatggcagaa aacaaaggtg tatcaataaa
     2701 agtagaaact aatggttcag caggtgttaa aaataagctt acaaaagatg aaatagaaaa
     2761 tgcaacatgt ataatagtgg cagcagataa aaatgttgag atggctagat ttaatggcaa
     2821 aaaagtcata aaaactaagg tggctgatgg tatacacaaa gcggaagaac ttatagacaa
     2881 ggctgtaaat ggagatgctc caatttacca tggaggagat ggaagtcaca acgaatcaaa
     2941 tgaagaaagt gaaagtggat ttagaaaagt atacaaacac ttaatgaatg gtgtatctaa
     3001 tatgttacca tttgttattg gtggagggat acttatagcg atagcctttt tacttgatga
     3061 ttatactatt aacccttcaa actttggttc aaatacacct atagcagcat tttttaaagg
     3121 aataggagat aaagcttttg gatttatgtt acctgtactt gctggatata tagcatactc
     3181 tatatctgat agaccagcgt ttgttgtagg atttgtagga ggtgctctag caggtgatgg
     3241 tggttcaggc tttttaggtg cactacttgc aggttttata gcaggttatt tagtagaagg
     3301 gcttaagaaa atatttagtg tactaccagc ttcattggaa ggaataaaac cagtattgtt
     3361 atatccttta cttggaacac ttttgatggg aattataatg acatttttag taatacctcc
     3421 agttacagca attaataatg caatggttgg atttttaaat ggtttaggtg gaacttcaaa
     3481 aatagtttta ggattagtat taggtggaat gatggctgtg gatatgggtg gacctgtaaa
     3541 taaagcagca tatgtatttg gagttgcatc acttgaatca gggcagtttg aaataatggc
     3601 agcagtaatg gctggtggta tggttccgcc acttgctata gctcttgcaa ctacattttt
     3661 taagaataga tttactaaag aagaaagaga ttctggtaaa gttaattatg taatgggatt
     3721 atcatttgtt actgaaggag ctataccttt tgcagctgga gacccacttc atgttatacc
     3781 agcttgtgtt ggaggttcag cagtggcagg agcacttagt atgttgttca acgcagctct
     3841 tcgtgcacct catggaggag tatttgttat accagtggtt acccatccat ttgcatatat
     3901 attagctata gctgtaggtg ctttggttgg aatgatgctt ttagcattac ttaaaaaacc
     3961 attaaatcaa gaagtatagc aattaaagga tattcaagaa cttaaaatat gatgttttat
     4021 aaaaattaat atattgctga attattttaa aataaggatt ttatgaggct gttatataac
     4081 gatttgtaat tgttatcttg acagtctttt atttttatat aagtaaaata agtgtgtttt
     4141 gctttatttg tatttaaatt gtttttaagt tagaatataa agaaaattat atattatttt
     4201 aatattcact cgaactagaa aataatattt gttgtatttt ttgtatataa aaatatattg
     4261 taatattaaa aaaataatag taaactgatt gcataaaaat gatttttata taaattttaa
     4321 acagaagaat aaatttctaa cgaaattgga tatttcttct gtaaatacat attcaaaaaa
     4381 agaattataa gagtttagtc agcagtcgtg taagtaataa ggactttata aatatataga
     4441 aatataatga tatgagtata agtagaaaaa ttttatgatt tttacaatta ttttttataa
     4501 aaaagggagg ttatgtttgt tgaatttaca atcaaaatgg gtaaactttt taaagccaac
     4561 atatgtcagt gatgaaataa aaagaaaaaa actacaagtt ataatatttt tttctataac
     4621 ttatggttta tcttattcac taggtttatt agcaatatgt tttaatcatt taatagattc
     4681 agaaatttta gctcaattta tgatgatatt gccattatct tcagtttcta ttgcaaaatt
     4741 ttacacagag ggaagaaata atgataaata taatttctat tcatcagtaa tattattttt
     4801 tattatttac ttttttatat ttattattga atcattaaag ttaataagta ttaatcaatt
     4861 tcaattaatg tatcttatgt taattttaat aagtagttta tgtgttattg tttattctta
     4921 taaaataaca agtttatata tttttaaaaa ttttaagata ggtattttgc tattttttta
     4981 ctttttgttt tctcaaatta tttttggatt aataataagt gataatcaat ttgattataa
     5041 gaatttcttg aattatattc ttcttcctat aatatcatta gtatacattt atcctttttt
     5101 atgtgaagag tatggttgga gatctttttt gcaaagtatt ttatttgata gatttggcaa
     5161 aaaaataggg attgtaatgg taggcacttg ttggagttta tggcacttac ctttacaatt
     5221 tacactatat agtcctcaaa ctccaataat tggagctata gccaatttga tatatggtat
     5281 tggactttct atatttttag gttatgttta tatgaaaact aagaacatat ggttttgctc
     5341 tataatgcat gttctaatta atagcttagg attaattttt aatgattctg aaataattat
     5401 taattaccat agtattatac aaagattgat atttatacta ttattttata ctccttttct
     5461 atttacaaaa gaatataagt aagaattaca attaatttat ataaaataaa tttttaagta
     5521 aatattttta tagagagatt gttatattac atatcagaaa aatattaaac taactaaatc
     5581 aataaaaaag ttggtggata acaatttata attgttagca caacatcatc ttatgtattg
     5641 aaatctaaaa tatcctcatt tttacttagc ataaatacta ttaattaaga aaataatata
     5701 gaaattataa atatatataa gtttagtctg gtgtataaaa caagaaaact aagtgatttt
     5761 taatttaact ttttattaag cttatttttt atactattta taataaataa actccatgat
     5821 gggaaaacta ttactattta atatgtaaag gagtatttta aatgaatgaa tcatattggt
     5881 ttttaaactc aagtccaaaa gaatataaca aattaggtga aaatataaaa acagattgtc
     5941 ttatagtagg tggtggaata actggattga caactgcata tttattagca aaagaaggta
     6001 aaaaagtagt gcttgttgag gctgataaaa taggatatgg aacttctgga aggaatactg
     6061 gtaaggtaac ttgtcagcat gatatatttt actctaagat agagaaaaaa tatggattag
     6121 ataaagcaaa atcatattat aatgcaaata atgaagcttt aaatttagtt gaacaaataa
     6181 tagaagaaaa caatataaaa tgtgatttta aaagggaaac ttcttttgta tttacagaaa
     6241 aagaggatac tataaaggat attaaagatg aatatcgtac atgtaaaaaa ataggaataa
     6301 attgtgagta tcatgaaact atagaaaata ttccattaga tataaagggg gctattagtt
     6361 ttactaatca aggtcaattt aacccaaaga agtatattga tggattggca aaagcagcag
     6421 taaatttagg gctaaaaata tatgaaaata caccagtagt agaccttgaa aaaggaaaaa
     6481 tatgtagggt taaaacaaga gaggataata taatagaagc tgagaatgtt ataatttcat
     6541 ctcatagtcc atggtatgat ggattgaatt tttattttgc aaaagaatat gcagagcgtg
     6601 cttatttaat ggttgcagtt ttggaaaata aattagcaga tggtatgttt ataagtatag
     6661 atgacccatc aataactttt agacaatata atgatggaag tgaaaattta ctgatttttg
     6721 gaggaggaga ccataaagtt ggtcagggag gaactgagaa agaaatattt gatgatttag
     6781 agcattatgg taaagaagtc tttaaggtaa aagattttaa ggggaaatgg tctgctcaag
     6841 acaatatgag ttttgacaat gttccttata ttggttatat caacaaaaga gaagacaata
     6901 tttatgtagc tacagggttc tctaaatggg gaattactaa tggaactgct gctggaataa
     6961 taataaaaga tttgataata aataataatt ctgattataa agatactttt aatccatcca
     7021 gattaggtag ctacttttca aaagatttta taaaggaaaa tgcaaacgta gcaataaatt
     7081 atgtaagtgg aaaattaaaa ataggtagtg gagatatgcc taaaaataat ggagaaggaa
     7141 agattgttaa tattgatgga aaaagatatg gtgtatataa ggatgataat ggagattttt
     7201 atattgtaga cactacatgt actcatttag gatgtgaact taatttcaac agtgaagaaa
     7261 aaacatggga ctgtccatgt catggttcta gatttgacta taagggaaat atacttgaag
     7321 gaccagctct taagccatta aaactttatg gacatggtga taatgatgtt aatcctaagt
     7381 tattataaaa ttatttttag gaaggtaaag tgtatatgaa taaactttta aagccaataa
     7441 ctatactatt gtatatagtt tatattttat ctgccgtatt ttttgtagca aacaaagaat
     7501 atggaagtct aggtctggta gcaattagtt tagttggtac tttcattttg agttttataa
     7561 ataagcaaac ggaaagatta ttgagtaaag aattatatat aatgcttgct ttatttataa
     7621 tgttttcatc attgttaggt acttgtttta atttttatga tataaattat tatgatgatt
     7681 ttttgcattt atggtcaggt ataatatctt gtattgtggc tttttctata attaaatact
     7741 tttatacaca aagagagata agtaaaatga gtaaagtgtt tttagttata tttttattta
     7801 tgtttagtat gggagtagcg agtttatggg aaataggtga atttttaatg gatacttttg
     7861 ttgggactac tacacaggcc ggagggttag aagatactat gattgatatg gtagatgctt
     7921 taataggcac aattataact attcctttta tagtaaaaaa atctaatata tagttttaaa
     7981 ttttttatat taattataat ggatttaatt aatcatgttg agtttcaagg tctatatgat
     8041 taattaaatc catttttgtt atttataatt attattgaat atatttttac atataagata
     8101 taaggtgata gatagaaatt gttggattat gtaaataatt aatatataat accattatgg
     8161 gcaataaata caaatcaaat gataatatag tctatttatg taaatgttat attgtatact
     8221 aagtataata gtaagttttt tgatagtggt gtagaaagta aaaatctact agcctaaatt
     8281 gatgtatgga ttagtttagg aacagtaatt gcatatctta agtttgatgt catgttgggc
     8341 tactagaaat aggtcatctt tttcacttga atatcttttc tttaaacatg gagagtatca
     8401 tatcaaaaac atatgaaaat taagtgattt ataagaacag taatatttta tatgatagta
     8461 tagaatagta ataaagtatt aggaggaaca ttgagtgtta aaaaagtggt ttggtattgt
     8521 gaaaaaaaga caaaaaagtg agtcagcaaa agaagaaggt gaagtaatat taaaaaacga
     8581 aaaaatatta tctgaagaaa agttgataga tgaagaagga gttgtagtta atattgataa
     8641 tgaaatatta actaaagtag aagtagtaaa tgatgataat gaaatagaag aaaaaataat
     8701 agaagaagat tggttaatta gtgaaaatac cataaaatta gatgataaag aagcaattat
     8761 taatgataaa aacatagaat tatgtaaaaa agaagttcaa gttgaaagtg aaaaaatagg
     8821 tttaaataag tttgaaggac ttgaccaaaa tgaaaattat aatctagaaa aaaatgttat
     8881 tgaagaaaaa aaagtaagtg aatgtttgac agaagaagat ttagagtaca taaaagaaat
     8941 taaaataaaa agaggaaaaa gtataaaagc tataaacttg tatactaaag aagagtgggt
     9001 ttttgacact catatacagt gtagtaaaaa actcaaagtt ccattagggt acataagaga
     9061 aaatttaaaa tatggatata tggattactt tggagatgca ataaattatt taagtgaagt
     9121 attaaatata gatgaatact gcaaaagtga atggagctat ctcgataata gcaaatctcc
     9181 atctgagata tttaatattc taaacaataa aatatttagt ataaggcttt ctaatgaaaa
     9241 aagaaatgaa attttgacaa atgataaaat tgaagcatta aaaatgaatt atagatttga
     9301 atgtattgat gaagaatatg atgaatattt taaaaaatat aagtctataa tcaaaagagg
     9361 tggaaagaaa aaagttgaat tagtaaataa aaaaggtgac attttagaaa tatttaagtc
     9421 tttagaagaa tgtgccattt atttgcaaaa ggaaaagaat gaagttatac aaatgttaaa
     9481 atatggagat acaaaagtag gaagaaactt tataaggtat agtttgagaa gtatttaatt
     9541 tcattttaaa ttcataagag tttcatttct gattcacaca tttaatatta ttttaatgta
     9601 aaataataaa ggtgaagtat tgcacaatta aaattaagga gggtattaat gtctgaagaa
     9661 aaaaatccaa aagaaaatac attagatgaa tctaaaaatt cagataatgt aaattctaaa
     9721 aaagatgatg tgtcagatga aaaaaatact gataacaaag ttaaaaaaag ggaaataaag
     9781 ctaaacaaca aaacacttaa agctattgta gcaattgttg cagtaggagc tatatgttta
     9841 ggtggaggct atgtttttgg caaagaagct ggaagaaaat tgcctgctac ttcaaaacac
     9901 tataatagca gtgaggtagt agctactatt ggggataaaa aaataacagg tgaaatgtta
     9961 agaaagagaa tggagccatt attttattta aatgcaaaaa ctactatgac tgatgacgaa
    10021 atacgttcat atgaatcaaa tatgattgaa actatgatta catctgatat attatcctct
    10081 gaagcagcaa aagagaaaat aagtataact aatgaccaac tagatgctca gtataataat
    10141 ttaatgagta gtatagaatc agcaatggga atgactaaag aagagttttt aaaacagttt
    10201 gatttgactg aaaaatatat aaaagatgat atgaaaaaag agctagtagc atcaaaatat
    10261 ttaaatgaaa aatcaaaagt tactgataaa gaagcagaga actattacaa taaaaacaag
    10321 agcaattatt tacaagttag agcttcacat attttaataa aaactgttga tgataaaggt
    10381 aagcaagtta gtagtagcaa aaaagctgaa cttaagaaag aagctgaaga aatacttaaa
    10441 aaagcccaag ctggggaaga ttttgctact ctagctaaaa aatactctga agatagtagt
    10501 gcagaatcag gtggagattt aggtttcttt ggtaaaggtc agatggtgga atcttttgaa
    10561 aaagcagcct ttgcattgaa gaagggagag gtatctaaca aacttgtaga aagtgattat
    10621 ggatatcata taattaagaa aactgatgaa aaatatcagc catttgaaga ggtaaaatca
    10681 gatttagtaa gcagtttaac atcagaaaaa caaaacctac ttatacaaaa tctaaaagaa
    10741 aaatataatg ttaaaattac agataaatat caaatgaaag ctaaagaata gataaagtca
    10801 atttattaaa aaaaagaaaa attaactgta tggataaaag ctatttttac tcttaatata
    10861 gagagtataa aatagctttt attattttgc aataaaatct atttatatat atgaagtgac
    10921 aaagaaataa catgcaaaaa gatattaaaa agaaatattt ttaatttgaa ttttttcaat
    10981 aaaaatttac aaaaatgaaa aaacaaacaa aaaagaattg aagatttaag ttgttattta
    11041 aatatatata tattaagatt ttctgctgaa gatactaaaa aaataatatt tttcataatt
    11101 ttaaaaataa taataaaaaa atgaaaatac aggcgataat ctattgagta aacatagaaa
    11161 agatgatatt ataagtatgt gctaaaatgg ttaaagcaat attaatatta aatttaacta
    11221 taaaacaaaa gggggcgttt ttttatgtca agtacagaaa gcgtatctaa aaaaagtggt
    11281 catccgaaga gtttttggct tatttgcatg acaattttgt gggaaagatt tagttattat
    11341 ggtttaacag ctttattggt attatttttt acagcaagtg tagctcaagg tggtttaggt
    11401 ctttctactc aagaagcaac atccatgttt ggcttattta caggtcttat atatcttaca
    11461 ccattagtcg gtggctggtt agcagataaa gttttaggtc aacaaaaatg tatttctata
    11521 ggtgcacttt taatagcaat aggagatttc gtactatttg cttcatctac atctattgca
    11581 atggtttggg taggtctatc aataattatt ttaggaaatg gtttttttaa atcaagttgc
    11641 tcaaatatag ttggggatat atatcctaaa actgatgtgt caagaaaaga tgcagcatac
    11701 agtttattct atatggcagt aaatgtagga gcatttatag ctccaataat atgtggattg
    11761 ctttctgata atgtatttgc agtaagagga gcaagtggag atatagtatc ttatggatat
    11821 aagatggcat ttttaatttg tggtataggc atgatgttag gtatgattat atttacactt
    11881 cttgcaccaa aattattaaa tgaagtcgga aaatatcctg tgactaaagg taataaaggt
    11941 gaaaaaattg agtatggacc acttactaag ttggagaaaa ataggataat agcaatggtt
    12001 attttatttt tatttgtggt cttattctgg acagcatact atcaaacaca aagttctttt
    12061 atgatttatg taagggataa tgtaaataga actctatttg gatttgaaat gccagtagca
    12121 tggcttacat cactaaatgc tatattatgt gtggctttat gcccagtatt agctacccta
    12181 tgggttaaat tatctcatac caaaagaggt gatttctcta ttccagttaa aatgggatta
    12241 ggaatgataa taatgggtgt tgggtttata gttatggtat ttgctacatt agctaatggt
    12301 ccagatggaa tggtaaaggc tagtatattg tttatagtat tgacttatat atttacaaca
    12361 gtaggtgaat tattcttatc tcctatagga cttgcaatgt tcaataaatt agctccagct
    12421 aagtatgcta cgttgtctat gggagcctgg tatctaacaa gtttttttgc aagtttatta
    12481 tcaggaaaga ttgctggttt tacagccaca cttggttttt tacagatatt tggaggaata
    12541 gcaacagttt taataatatt tggtttaata ttattgggaa taagaaatgt attaacaaga
    12601 ttaatggcta tggatttact aacaaaagaa aaataattca tgacgaatta ttgtaaataa
    12661 atgaattact ttaaataaag gaagttacat ttaaatataa aattaaatgt aatttcctct
    12721 ttgattatga aaaaaagctt tacacaaata gatattataa ttaaaattat aattgtgata
    12781 attagagtgt ataagattta tataaattat acaaaaaata tttatgtaat aaactttaca
    12841 tacttatata tttagttatt caaactatta tttcgaggag gtcttgttgt gaatataaaa
    12901 gatagattaa gtggattaag aaaatttatg gaagaaaaaa atatagatgc ctatatgatt
    12961 ccatcttctg ataatcacca aagtgaatat gtaggagatt acttcaaatc aagagaattt
    13021 atatcagggt ttaatggttc agcagggacg gttattgtga ctaaagacga agctgggtta
    13081 tggacagatg gaagatattt tatacaggca gaatctcaat tagaaggaag tacaataaaa
    13141 ttattcaaaa tgggacaaga aggttgccca actacagatg aatatttata taaaaatatt
    13201 cctgaaggag gaactttagg atttgatggt agagttatat ctgctagaga aggagctaca
    13261 ttagctgaaa aattatctaa aaaaggtata aagatagaat atcagtatga tttaatagat
    13321 agtatatggc cagatagacc tgctttatca gatagtaaag catttttatt agatgttaaa
    13381 tattgtggag aaagttttag ctcaaaatta gctagattaa gagaaaaaat gagtgaaaaa
    13441 ggtacttcta ctcatgtaat aactactctt gatgatatag catggttatt caatataaga
    13501 ggtggagatg ttaaatataa tccagttgta ctttcttatg cagtaataac tttaaaagaa
    13561 gtttatctat ttgttgatga aagtaagtta aatgaagaaa tactaaatga actagctaag
    13621 gaaaatgttc aaataaagcc atataatgat gtgtatgaat ttgtaaaaaa tatagacaaa
    13681 actgaaaaag ttttattaga tggaactaag cttagttata caatttacaa taatatacct
    13741 tgtgaagttg agaaagttga tgagttcaat ccagtaatgt tttttaaagc tcaaaaaaat
    13801 gaggtagaat tagaaaatat aagaaattca catgtaaaag atggtgtagc tttcactaaa
    13861 tttatgtact ggttaaagaa aaatgtaggt aagatggaaa tcactgaaat aagtgctact
    13921 caaaaattag aggacttaag aagagagcaa gaaggtttct ttgagccaag tttcaataca
    13981 attgctgcat ataaagagca tgctgcaatg atgcactata gtgcaactcc agagtcaaat
    14041 tataaattag aggcagaagg attattttta gtagattcag gtggacaata ttatgatgga
    14101 actactgata taactaggac tacagtttta ggacctataa gtgatgaatt aaaactacac
    14161 tttacttctg tagcaagagg aatgataaat ctttctaagg ctaagttctt gcatggttgt
    14221 agaggatata acttagacat actatctaga agttgcatgt ggaatatggg aattgattat
    14281 caatgtggaa ctggacatgg tataggattt gtgctaaatg ttcacgaagc tccaaatgga
    14341 tttagatgga gagtagtacc tgaaagattt gatagtgcag ttcttgaaga aggaatggta
    14401 actactaatg aaccaggtat atatatagaa ggttctcatg gtataagaac agagaatgaa
    14461 attgtagtta gaaaagctga aaagaatttc tatggtcaat ttatggaatt tgaagttgtt
    14521 acgttagctc ctattgactt agatggtata gtaccagaat taatgaataa ggatgaaaaa
    14581 gattatttaa actggtatca taagttagtt tatgataaaa tatcaccttt cttaactgat
    14641 gaagaaagag aatggttaaa agtttacact agagctatat agtttgtact agatacaata
    14701 aaaatcgagg tattataatg cctcgatttt ttattgtata ttttattaac tttaaaaaat
    14761 ttttaatttg ataattctac taatttttta ttcattataa gtagtataat tccaaatatc
    14821 aataaaaatg atggtatcac tatgaagact tgtaacatac caagttggtc tataatagct
    14881 tgcgtatagc cagataattt atttgctgca aatttgccta aaaaccaaac acccattagg
    14941 aatgaaatat acttttcagg cgaaagttta tttaccatac catacccgac tggagaaaag
    15001 caaatttctc ctatactttg gaatgttaaa aatccaacta cccaaaatac acttgctttg
    15061 tgaattgaat tagggtctgt tcctgtttgt ataactgcta ctatcataaa caaaaatgct
    15121 atggctaata gtataaaacc taagctcatt ttttttggca cacttaaatc gcctttttta
    15181 gtttgagata attttaccca tactaaagca gagataggtc caagaataac acaaaggaga
    15241 ccattgtatg aatctatcca tgaagtagga atttgaaaat tgccaacagt aagatttatg
    15301 tgttcctttg tatagagtgc aatagacata gaggcttggt tatatgctat ccaaaaaaat
    15361 actgtgaaaa aagctaacac caatattact attattcttt ttttctcaat caaagttaaa
    15421 ggtcttgaag ctagcatttt cttatattct agtgattttc ttatagactc tctcccgtta
    15481 ctagggtcat tattcaaagg tttataaaac tttctgtttc caaatataaa gaagaataaa
    15541 cctattagaa ccgtaattga tgaaaataaa aatacatatc tatatccata catacttata
    15601 gataatccgc ttttcatagc aaaccattca ttagcaacta aaccgcatat taaagggcca
    15661 aataaggtac ctagattagt agccatatac gtaatagaga aagctccatc ttttcttggg
    15721 tcatcattgg tatataagtt tccaaccata gtatgtaagt ttcctttata aaaacctatt
    15781 cctaatccag ataaaaatat catgatgttt atatccatct tactatttgc tatgtatcct
    15841 agaaaataac caattgaaac cattagcatt ccaagtataa tacagttttt gggacctaaa
    15901 aatctatcac tgacccatcc accaataact ggagagaaat aagtccatgc taaaaaagat
    15961 gcaagcattg atgcggattc tgctggtgtt aaccctaaac cacctttgga gacttgttca
    16021 gctaaaaaca ataataatac caatttcata ccatagtatg caagtcctac aaaggtaaat
    16081 attgaacagc agaaatataa atctctaggt tgtttaagtt tttcttgcgt ttttaaaact
    16141 gacatatgtc gagaacctcc agataaatag tttaacaaaa ttttaccata ctatatgagt
    16201 atatgttttt ttcatataga cgtcaacttg taagataatt taattaagtc gaaatatatg
    16261 taaaaatagt atcatttttg tgtattcagt aatatttttt tatgataata tatgttatta
    16321 ttatattaaa atacaaacat atttatggaa aggatatgcc aatttagtag ttagattgat
    16381 tgtttggcag gtgggatgag ctatgataag aaagaaatct gaattacaag tagaagtaaa
    16441 agaaaattta aatggtggta taggaaaggt taagcttgaa aacataatac aaaatgaaga
    16501 gcttaaagga aaaggaagac tatttaaaag agtagcttta ccagtaggaa gttcaattgg
    16561 tgtgcatgac catacaacag attttgaagt atattatata ctaaagggaa aaggtaaagt
    16621 atttgataat ggggaatttg tagaagtaaa tgagggtgat gtagtgtata cagctgatgg
    16681 cgaaaaacat tctatagaaa atataggaga agaagatctt gagtttgtag cactagtttt
    16741 atatatgtaa aatatggagg aggagtcatg acaggcaaga tagaagaaat taaggacaag
    16801 ataaatagat tttgcaactc caatttaaat caagaataca aagatataag ctttaaaatt
    16861 ttgcaaaacc ttttagataa taataaagag ataattcatt cttctagagc tgatatatgg
    16921 tcttcagcta tactaaatat agttttagaa caaaatcttt tatacaataa gaagcatcca
    16981 ctacatataa caaaaaaaga gttttcaaaa caaataggtg tttctttaaa tactataaat
    17041 aataaatcgt ctttaataaa agatgtttgt agtattgact ttttaaatca agatactata
    17101 gctatttcta atgtagaatt ttgggtaaaa gagaccaatt ccaaatcaaa gtctgtagat
    17161 ttagatttag aaaagaaaaa aatactatat aaaagatata ttaagctatc acaagatgcg
    17221 aatagtgata ttgaatcaat tagatatatg gaggaagctg taaatatagg taaaagtatg
    17281 attactaaag aagataaaga acttggattt tggaaaggtt tatctaccag agcctatatg
    17341 atagcacttg aatcccttgc tagaaagtta gaaagtatta ataatctaaa ggagtccaaa
    17401 aaagtattag aatatttaat agaaattaat cctagtgatg agcagggaat aagatacaaa
    17461 ttattaaatg tattaattag attaaatgat agaacttcaa taaataaatt atttgaactt
    17521 tataaagaag aaaaatctgc tacatggatg tacagtaaag ctctttatta ctttaaaaat
    17581 aaaagtatgt ttttggcaaa tgattctatt aaggttgcta gaagtaaaaa taagtacgtt
    17641 ggattatatt tgttagattg gcgaaatgct tttggtagag aatttgttac agaggaagaa
    17701 aaggcagaag ctgtctatta ctatgatgaa aacatagtga tttggaatga agttaaaggt
    17761 tctatggatt ggctattaaa aaaaatgttg gaattttcat aaaaaaaact actccttact
    17821 tttatataag taaaaggagt agtttttaat ttatacatct agttttaaat cttcaggttt
    17881 tattagacct ttttttctca taaattctga aatcaacaaa gttacaatag caggaagtat
    17941 aaaattaagt aaaagtatac caacccacat agatatacct ccttttccag cactttgcat
    18001 agcagttact gttccaaatt gtccaaccaa tccacaagta cccataccag aaccaatagg
    18061 aatattctcc attttgaata ttgttgtaga taatggtcca agtaatattg aagctacagt
    18121 aggtggtatc caaattttcc aattttttat tatattaggt acttgcaaca tagatgtccc
    18181 aagaccttgt gccacaaggc cgccaactcc attttctcta aagctcataa cagcaaagcc
    18241 taccatttgg caacaacaac ctacagttgc tgcaccacca gcaagaccac ctaaacttaa
    18301 catcatacaa atagctgcac tacttatagg taatgtaaga gctattccaa caagtgcaga
    18361 gactaaagct cccattatta taggttgcat agttgtagct tgcattatta tatttccaaa
    18421 tgaagtcata aaagcagaaa cagctggacc aataagttca gctacaagta ctccaattag
    18481 tattgttaca gcaggtgtaa ccaatatatc tatttttgtt tcttttgaaa ctaattttcc
    18541 aacctctaca gcaaaaagtg tagcgacaaa tgctccaaca ggtcctccta attcattacc
    18601 acaagctcca acaagtgcac aagaaaatag tacaagtgga ggtgcttgaa gtccatacgc
    18661 aatagccact gctatagctg gaccagtaac ctgtttagca agagggctta taacttcagt
    18721 aaaaagagaa atattgaaat tatctcctaa tgtattgaaa attgttccaa ttaaaaggga
    18781 cgaaaaaaga ccgagtgcca tataacttag tgcgtcaata agataccttt gaacagaaat
    18841 ttcaatattt tttcttttag caaatgattt gaaatttctt tttttataat tgatttccaa
    18901 tttaaaatcc tcctcatata atataagtat aatttctttt taattaatta tataactttt
    18961 cagctgacaa gacaaatgtt atttcactaa aattagacta ttgtaatagt aagaaattaa
    19021 cattaatttt agttttaaat tttaaattta tggtttataa ttagtatagg gatattctga
    19081 aatataataa ttttccagtt tatcccaata tttaacttag gtagattgct tacaaaacaa
    19141 atgattatat attaggctaa aaggaagtgg atagatgata tttgtaaaaa ataagaaata
    19201 cagaaaaatt gccataatta tatctattcc tatattgctt gttgcgcagt actacctttt
    19261 taaatttgga atacttagac caatggtaaa gggagtagaa attgaaattg tagatgggga
    19321 atatgttaaa gatatagata aatatgtagt aaaattaaat gaaacagtta ctttgtctac
    19381 aggtgaatat attaaggttc cttcgtatgc taaaaatcca aatatttggt ttaatgtatt
    19441 ggataactca gggactttaa aaatagatgg aaataaaatg acagcattaa aggttgggta
    19501 ttcttctgtg ggtatcatga aaaattcaag agtattaaaa aaggctacta taaaagtagt
    19561 agacccagag gtagaatcac tagatatgga gattaatggg gatttaaaac atgttggtga
    19621 tagtgctact atagaaagta caatagaagt tgattatgat aagtttaaaa aatcatataa
    19681 gccagtttat aagagtagca atgaaaatat tttaaaaatc aaaggaaata aagtaagtgc
    19741 agttggagtt ggtaaagcta caatatatgc taagtgtgga aataaagaaa ttgagagaac
    19801 atttaggatt caagctagag ttgctgaaat aaatatggat gatataaaaa tagaagttga
    19861 ccagaatgaa aaattgaaac cagatataat aacttctcca cctggtttag aaccaccaaa
    19921 aatatattat gagttagtag attcaaaatt acctgtagaa agagcaataa gtttaagtag
    19981 taatggaaat gttgtagggc ttagagaagg taaagatagg gtgagaataa cttgtggaga
    20041 taaatctaaa gtaataacag taacagtagt caaagaaact ataacgaatg actatataca
    20101 aaacttggtt ggttcacata aaatagtaaa caataaagtt attattaaac ttgaatggga
    20161 ttatatgaaa gatgtctttg attatgatat atatcttaaa gataagttgt caggagaatc
    20221 atttaagaaa attaaatcta taactataaa tgaaagtgat ttaggtaagt ctaacaaggt
    20281 atatgcaact atagaagtag atatgaaggg taatcaaaat atagattttg atatttatgt
    20341 cataggtaaa aaggattcag atacaactaa gccaagtaat gtaataaata ttaccaatgg
    20401 aaatggaaat tcaaataatg ataataatat taaagatatg agggtagaaa atatatcagc
    20461 taatgttgat agagataata atgtcattag agttaactgg agtagtatat ctcataagga
    20521 ctgtacttac agtatatatg taaaaaataa tactaatgga gatggagaat ttactctata
    20581 tcaaagtgat atacagggaa atgagcatac tataagtatt cctgaaggag atttaaatct
    20641 agatatttat gttgttgcaa attataatgg aaatagctct caaaatagtg atgttataaa
    20701 tgtaagatag atttgtatac aaattgaaag tgttgagttt atttaaatat taaattaaga
    20761 aaaaggatag tatagggtaa ttatactatc cttttttgtt acaatatata ggtttaaaat
    20821 ctctagtaat ataaattata aaaagtctta tttacacaaa tctatagata ttttttttat
    20881 taatcaatat tgtcttaaat tgaacttagt gcggcttcta ttgcatgtat tactgtcgta
    20941 tcaaatagtg gaatagaagt gtcattttgt ttaattaaaa gacctatttc tgtacatcct
    21001 aatataacag cctctgcacc ttgtttgcca agcttatcta taatagataa gaaagctttt
    21061 tttgaagatt cagatataat tcctaagcaa agttcattaa atattgtgtc atttactatt
    21121 atacggtctt cttcatttgg aattaagact tcaattccat tattaataat tacattttta
    21181 taaaaatctt gttccattgt atatttagta cctaataagg ccactttttt tatttctttt
    21241 tcccttaata ccgtagcagt tacatctgct atgtgaagta atggaatatg tatactttct
    21301 tgtattttat ctgatacctt atgcatagta ttagtacaga taacaataaa atctgcccct
    21361 gcctcttgta atttaattgc agcatcagct aaaattttgg cgcttttttc ccaatttcca
    21421 ctagattggc attcttcaat ttcttgaaaa tctacactgt aaagtataca tttagaagag
    21481 tgtagtccac ctaatctttc ttttattact gtattaataa cttgataata tgtaatggta
    21541 ctttcccaac tcataccacc aattaagcct atagtcttca tattatacct ctcttattta
    21601 tttattttga aatagctatt tacaaataca agtatagcat ctaaaataga agcatacaat
    21661 aataaaattg tatggagtac aattataatt aagaggagta attatatgcc aataaattct
    21721 tttgaaaact atcctatgac atggaaacca aagttagata accgaaaacc accaatatat
    21781 aaaactttag caatgttatt agaagaagat attaaaagag gaaatttaaa tcctggagat
    21841 aaattgcctc cacaacggga attagcagat tttctagatt taaacttaag tacaataaca
    21901 cgagcattta aactttgcga agaaaaggga ctaatatgtg caaaagtggg aaagggaaca
    21961 ttcatttctt ctgatgtaaa tgtaagtaat acattattat atcaaacgga atcaaaagac
    22021 ataattgagt tggggacagt tcatcctccg tatgagcaaa atacatatat tattgacttt
    22081 attaaaaatg tactaaaaca accagaaatg gataggtttt tacaatacat gtctccatct
    22141 ggcacatata tgcaaaaaaa gtcaattgca aaatggatag aaagaaataa tgtatacacc
    22201 agtgaagaaa atatattatt atctacagga gggcaaaatg ctatatgtgc aactttacta
    22261 ggattattta aagctggtga tagaattgct acagattctt taagtttttc tggaattaag
    22321 tcaatagcta aaatgatagg tattcaatta gtcccaattc ctcaagaaaa taatgaaata
    22381 tcgattgaat atcttgaaag ttattgtaaa aatgaaaata taaaaggcat atatcttata
    22441 ccagattatc ataatccaac gacacatact atgtctgatt tatctcgtaa aaagatagcc
    22501 aagatagcaa agcaatataa tataataatt attgaagatg ctataaatag catttttaga
    22561 gatggtatac aaactcccat attttcttta gctagtgata atactatata tatttttagt
    22621 acatctaaat ttttatgtgc tggtcttaga gtagcatttg tagttgctcc aaaacgatat
    22681 atagaaaatt tagaaaatgc actatataat atgaatctaa tggtttctcc atttacagca
    22741 gaaatagtac accgtttatt atattctcct attatagata aaattataga agagaaaaga
    22801 gatgcaatta tagaaagaaa tgaagttgct gataaagttt tgtcagatta taatttgatt
    22861 ggtgataaga attgcagttt tcgttggtta cttcttcctg atgaattgga tggaaaatca
    22921 tttgaaattt ctgcaaaaaa tctaggagtt caagtatatt gtgccgaacg attttcagta
    22981 ggaaactcta cagttccaaa agcagttcgt atttgtgtaa cagctccaaa agatgtagaa
    23041 gaattagaaa aagggcttaa tataataaag tcacttctta agagttaaat ttattttact
    23101 attatttaat taaataaata aaaaggcgga gaaatttatt ataataaacc tccgctttta
    23161 ttatttaatg aaatacttta ctttttatta aaaaatttaa ttttaataat cttgaaaata
    23221 taatcgtttt atggtaaaat aattcttaaa aatagaaaaa attaatcatt tgttaaaaaa
    23281 aatgcataat ttattgtaat ttattgacga aggttgaaaa atattcataa atgtgcgaaa
    23341 taacagaaaa aatgcaaaaa ttaattcaat ttcatttgtt ataaaatcta tatggcaaaa
    23401 ttaaatctgc ctatcagaat gatataaaca catcagaaag gaggacgcta tcaaataata
    23461 caagtagtta actttgatag caaaaaatta tgaatgaaca aactagaata tccttagaga
    23521 gagctgctga attaaaatca aaaattgatg attatattca ggctagaaaa acgattaaca
    23581 gaggtcttga aaaagaagaa gagataaata aacgaaaaca gaaaatatta agtatcttaa
    23641 atggaactga agaggattgg aataactaca aatggcaatt atcaaataga ataacagatg
    23701 tagatacttt atcaaaaatt ataactctaa ctaaaaaaga aaaagaatat ataaaagagg
    23761 ttggtactca atttagatgg gcaatatctc catattattt gagtcttata gacccagaag
    23821 atatatgtga cccaataaaa ttactgtcta taccaacaca tatagagttg gaagatgagc
    23881 aagaagattt ggacccaatg ggagaagagt atacaaaccc agcaggatgt ataactagaa
    23941 gatacccgga tagattaata ataaatgtaa caaatgagtg tgctatgtat tgtagacact
    24001 gtcagagaag aagaaatatt ggacaacaag attctcataa gtcaaaagct attatccaag
    24061 aatctataga ctatatcaga gaaaatgaag aaataagaga tgtactagta actggtggag
    24121 atgctcttac attaaaagat gattatttag agtggattct tagccaactt aaagagatac
    24181 cacatgttga ttatgttaga ttaggtacta ggactcttgt tacaatgcca caaagaatta
    24241 cagatgaatt ttgcaatatg ctaaaaaaat atcaccctgt atatataaat actcatttta
    24301 atcatccaat ggaaataact aaggaatcta aagaagcttg tgaaaagtta gcaaatgcag
    24361 gcgttccatt aggaaatcag gcagtattat taaatggaat aaataatgat aaatttgtaa
    24421 tgagatgttt aaatcaagaa ttactgaaaa taagagtaaa accttattat atattccaaa
    24481 gtaaacatgt aaagggaaca aaacatttca atacatcagt agatgatggt cttgaaatca
    24541 tggagtattt aagaggatat acatcaggaa tggctatacc aacatatata gtaaatgctc
    24601 caaaaggagg aggaaagact cctttgcttc ctcaatacct tgtatcaaaa ggaacagatt
    24661 acgttatgct tagaacatgg gaaggaaaag ttataaaaat ggaagatgaa cctgctgtag
    24721 atataaagaa acttataaaa gaacaagcac aggattaaaa aaatatttaa agtttataaa
    24781 aagaaacaac tttaaaataa aaagaaaatg gatgtcaaat ttatatcaat ttgcatccat
    24841 tttttattag ttatttatta ttttgttgtt gattaactaa ttcaagatat ccacttgggt
    24901 ggaagtaata tatctcagaa gtattttcat ttacaaagta accaatatca ccatctgcat
    24961 ctggtaaaaa tacataacca ttaagatttt tagcttttaa tgcttcaaaa tcactagcat
    25021 ttccttgata tatgtaatca acttcattgt tgtattttac taaaagttct ttagcttgtt
    25081 cttgagtcaa tttagtattt tggcttacag gaacattctt ttgagaatct gcataagaag
    25141 attcatttac tggtgcagtt aataacaaac aagataaaac taataaagat ataagtgctt
    25201 ttttcatact aaaaccctct ttcatgttta attatataag attcattact taataatatt
    25261 gtatgtttcc tataattaaa tattaccatt aaagagaata atattacagt tacacagctg
    25321 taacttttga ttacaaaatc gtaattaaat tgtattttta ttccatttgt attttatcat
    25381 gtctacgatt tacaaactca cagaagtata tctttataac gcccatcaca ggagcagata
    25441 gaatcatacc aggtatacca aagaatcctc caccaacagt aactgctagg attgtaaaga
    25501 atgggctaag acctagttgt ccacctacga tttttggctc tataaatgta acttcaactt
    25561 gttgaattat tattaagtaa attaaagaag ttaaagcaat tgttggattg gagaataaat
    25621 taattattac aactggtgcc atacctatta ctggaccaaa atatggaacc atattcatga
    25681 cacccataag tgtaccaaat aatagtgcat attcagattt taaaaagtaa agaccaattc
    25741 cagaaagtac tccaactata aaagaatcta aaatcttacc tgtaaagtat ttaccaatat
    25801 ttaaatttaa gctttgacat agttctatta taaagtctcc atatttttta cctaaggaaa
    25861 tataaactac tttttttgta aacagtaaga atttttcttt ttctaataat atatagaaac
    25921 aaattataaa acctaaaaga aattgtacta taaattttcc aactgaaaat gtagtgctaa
    25981 atatcccacc aagtgaacca ataagcaagt tactaaattt aggcattaca ctcataattt
    26041 tgtcaccata ctcttttaat gtattagggt caactgattg taaactcttg cctaagtcga
    26101 ataaaaactg ctctgtttta tctatgtatg taggaatttg agcaactaaa tcagctaaac
    26161 tatttactat ggatggaact gtaaataata taaacgaaat tagtataact atgagtacac
    26221 cataagtaag aagtaacgaa tatattctct taaaatttaa tttactttct aagaacttta
    26281 ctagtgggtt gaatatatat gctaatacaa atgctattat aaatggtgtt agtagtgaca
    26341 ttaataaact tatcactcca aaaaaatatt tgtaattatc aataacttta atcaagatat
    26401 atgaaacaac tacagtgagt acaatattta gataaatttt tttatcttgc aaaataacac
    26461 aacctttctt atatttatta aatttagata tacttaaatc taagttaaag ttaatattta
    26521 tag
//
LOCUS       DAEPIN010000056        26147 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.056, whole genome shotgun sequence.
ACCESSION   DAEPIN010000056 DAEPIN010000000
VERSION     DAEPIN010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 26147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26147
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.056"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            351..2450
                     /gene="nrdE"
                     /locus_tag="KR250_002627"
     CDS             351..2450
                     /gene="nrdE"
                     /locus_tag="KR250_002627"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HBH0068549.1"
                     /translation="MNWIELNNQVIIKNEQGKYQLEKDKEALSSYIEEFIQPKLRKFN
                     NLEERLKYLIDEGYYSKEVINQYSIDVIKNIYDMIDKHKFEFQSYMSANKFYQNYALK
                     SNDGKEILETYNDKVLIVALTLGNGDKDLALNLADKIVKQEFQPATPTFLNAGRKRAG
                     EMVSCFLLSVEDSTEGISYAISSSNHLSKIGGGVALNLSKLRASGESIKDIEGAAGGV
                     VGVAKMLEQSFSYFNQMGARQGSGAVYLTVFHPDFELLMDTKKINADEKIRLATLSLG
                     AIIPDKFMELAEKNEVAYAFYPHTVYKKYGVSLDEIEMDEWYDKLVNDSDIRKKEINP
                     RQMLTKIAQMQQESGYPYVVYIDTANREHTLKDVGIIKMSNLCCEIFQYQTPSEIEGY
                     GGKNEWGQDISCNLGSLNIANVMDNKTIESTVETAIRALSFVADSTDIKPVPTVSNSN
                     SKSHSIGLGAMNLHGYLVRENILYTSDDAIDFSNVFFAMVRYYSIKASMKIAIERNQT
                     FEGFDKSEYVKGRNSKVLSKYYEQSYLPKSEKVRALFEGIYIPTKEDWTKLLDEVKEK
                     GIYNAYLMAVAPTQSISYVQNATSSIMPITEPVEVRTYGDSTTIYPMPFLTNDNMLYY
                     QSAYRMDMRKVIDLVATVQNHVDQGISTTLFVTDEKTTRDIARHYIYAYKKGLKSLYY
                     TRTKMTRDTHECLVCSV"
     gene            2462..3433
                     /gene="nrdF"
                     /locus_tag="KR250_002628"
     CDS             2462..3433
                     /gene="nrdF"
                     /locus_tag="KR250_002628"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HBH0068550.1"
                     /translation="MTFNLHKIHNAVNWNREEDGFTQAFWEQNVKQFWLPEEISVSKD
                     IKVWNELSNKEKELYKKVLGGLTLLDTKQGNNGIPSMMSLTENLQRKAVLSFMGTMEE
                     IHAKSYSSIFMTLLSNLEIDELFEWIETEPTLQRKADLVLAQYENTTNQEGLYLSMVT
                     SVFLESFLFYSGFFYPLYLSGQGKMVASGEIISLILRDESLHGKYIGLLAQEIYDSFD
                     KMDKEMLEEKMYSILYSLMENEIEYTNVIYRESGLEKEVVNFLKYNANRALENLGFEK
                     LYTVDAINPIVLNGLSTETKTHDFFSTKGNGYQKGVYEELEDEDFII"
     gene            3509..4135
                     /locus_tag="KR250_002629"
     CDS             3509..4135
                     /locus_tag="KR250_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HBH0068551.1"
                     /translation="MKYEWRKRDKELYLPKKNPVIVEIPEQKFIMLKGSGDPNSKEFT
                     EAIGVLYSLAYAIKTMPKKGIVPEGYFDYTVFPLEGIWDKGEKSKGLDVLIKEDLIYT
                     IMIRQPDFVTQDLLDKATDIVKKKKPHNLLEKVRFGSINEGLCVQMLHIGSYDNEPES
                     FDIMNEFCKKNKLIRKSYTHREIYISDARKVSPESLKTVLRFEVEREV"
     gene            4530..5159
                     /locus_tag="KR250_002630"
     CDS             4530..5159
                     /locus_tag="KR250_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0068552.1"
                     /translation="MNKYLLINPVAERMYGEKFHLIKANLIKKGYIITECEPQLEYVK
                     KQYKEYTKKTENTMLDCRCPESISLLKRNNLINNFEVPMIEPILVRTCRVLYDKYIKS
                     KDDMLVVTCPCTQLRDFASEKFKDKGNAMFYTWKEFAEQEGVKSLGKIDESPIPLGYF
                     KDTFEKVLEVSSEDEILSEIQKIRNGFEDKYDIVEMFYCKDGCNNGDGL"
     gene            5160..5942
                     /locus_tag="KR250_002631"
     CDS             5160..5942
                     /locus_tag="KR250_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0068553.1"
                     /translation="MVNKFNNKFLRLVKSVLIFFIILLLWKITNYLGIWSDYILPSPE
                     KVYSTFLNMISDGSIFINVYASMKRVLIGFAISTAIGVPLGIFFGIYSGVYEYFKSLI
                     NFLRNTPPLALIPMLILWFGIGEESKIIIIVLASFFPIFTSTLKGIKNCDSKLIEVGR
                     VFEFSKLQIIFKIIIPNAILDIAVGLKLALGYSFRAIIGAELVAASSGLGYLISDGKE
                     MSRTDVVIVGIIVIGLLGIITDYIFSIIVKKVSKGKMVEAYE"
     gene            5935..6813
                     /locus_tag="KR250_002632"
     CDS             5935..6813
                     /locus_tag="KR250_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0068554.1"
                     /translation="MNNLVCDSGYIIRNLYKSYKVDNKEHVVLSDISLNIDRNHITVI
                     LGESGCGKTTLLRAIASLEGINSGEIRFINDNKEYRPNVGFVFQESRLMPWLNVSENI
                     AFHNQRKNTILNKVLRKFKHNKRIRSKIINDNFRSLETNDNLNKSNKYNRKSNIDVEK
                     YLKMMNLQKFKNSYPNELSGGMAQRVSIARALSFNPDMLLMDEPFSALDYFTRIDMQN
                     EVIRIHKSTNKGVIFVTHDIDEALKIGKKIVVFTDERKVKEFNIEDGYNRDLTSDYYI
                     KLKKDILMTMKSNKGI"
     gene            6846..7805
                     /locus_tag="KR250_002633"
     CDS             6846..7805
                     /locus_tag="KR250_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrtA/SsuA/CpmA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HBH0068555.1"
                     /translation="MKLKKLLIGITILTLATASLAGCAKKNNGEKLSEINLTYVKSPL
                     NVPSIIQKQDDLFGKEFKKDNIKVNFHEITTGPEQTQALAAGEIDFLHALGGTSALIA
                     ASNGVELKILNTYSRSPKGFMILTNNNSIKSAADLVGKKVAGPKGTILHQVLISALDK
                     EGLSMDDVEFVNMGIPEASAALSDGSVDAALIAGPAALKAMKSGSKLVANGEGLVDGI
                     IVTAVSTDFAEKHPEIVERFMKVEKETLEYVNNNFDEAMEKVAKEVDLSLEETKELYA
                     WYDFSLDITDKDISSLEDTQDFLIKNKLQEKKVNIKELIYNAK"
     gene            8119..8811
                     /locus_tag="KR250_002634"
     CDS             8119..8811
                     /locus_tag="KR250_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0068556.1"
                     /translation="MSYKVYIVEDDISISTLLKDYIEKYGFEVIVEENFDDIMITFES
                     FKPHLVLLDVNLPKFDGFYWCRCIRQQSNLPIIFISARDGNVDQIRALESGADDYITK
                     PFYYDVVMAKIKSHIRRVYGEYSPKIDERIVELDGLKFYPERLELELNGENLIITKKE
                     GILIECLIKRYPKVVSRDYLLEKIWDDIEFVEENTLNVNVSRIRKRFEELGIDNAVQT
                     VRGLGYKLNKNW"
     gene            8821..9825
                     /locus_tag="KR250_002635"
     CDS             8821..9825
                     /locus_tag="KR250_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBH0068557.1"
                     /translation="MKLFLKDYKGYIFIYYIGIVMTIIYCNLMKFISFGESLYILLFS
                     SFILLCFLIYKYYKNREVYNLFEKGVHSLEESFLYLGSSFLGRNISVILKQQHKLYQS
                     MIQEHKKIHSEHLTFINQWIHQMKTPISVISLQLQDYEGEEVTENIRKEIDRLNKGLD
                     MAMHFARADNFQKDFIVEKAKLNEIVSSIVNEEKRLFIKSGMIPKIQVDKFTYVYTDV
                     KWMKFIIGQLITNGVKYSKDYSNYLTIKSEENNKYVILSIIDEGIGIVSKDLKRVFAP
                     FFTGENGRKFGESTGMGLYLVKKVCDDLGHKVSIKSEVGKGTEVSIYFNKGVNSLIKM
                     "
     gene            10073..10924
                     /locus_tag="KR250_002636"
     CDS             10073..10924
                     /locus_tag="KR250_002636"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HBH0068558.1"
                     /translation="MSLDVIFILKSVIIAIVEGLTEFIPVSSTGHMILVGNLIDFKGQ
                     FAEMFEVVIQLGAILAVVVLYWKKIKDSVIEFFKFIFTGGKEGKIGFRFGMNVIIGCI
                     PFAIIGVLFYDNIKSLFNLQSVIIGFIVGGILLLVVETLFRKKNHSTDNIDKITPIQA
                     LKVGTLQVLSAWPGMSRSASTIMGGWIAGLNSPTAAEFSFFLAVPAMVASSGKDLFEF
                     DYSIMTPTLWIALVVGFIVAFIVSIIVMEKFVNFLKKKPMRVFAVYRIIMGVVLAVLA
                     FTNIISV"
     gene            11028..11774
                     /locus_tag="KR250_002637"
     CDS             11028..11774
                     /locus_tag="KR250_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001637630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0068559.1"
                     /translation="MKILTVNNLSKVYGKKIIFNALNDINFSIEDGEFVGIMGPSGSG
                     KTTLLNMISTIDKPTTGTMELKGKNPLLLRGEELALFRRRELGFVFQDFNLLDTLTIG
                     ENIVLPLTLDKVSVKEQDERLNEVSTILGIKDLLGKRTFEVSGGQAQRTAIARALINN
                     PSILLADEPTGNLDSKSSKVVMELFQKINKENKVTTMMVTHDPLAASYCSRILFIKDG
                     SIYNEIYKGSSREQFYQEIMDVLTLLGGDN"
     gene            11774..13690
                     /locus_tag="KR250_002638"
     CDS             11774..13690
                     /locus_tag="KR250_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0068560.1"
                     /translation="MNIKQFAINNISRNIKAYLGYLASIVISSSLLFSFIMFTSHPDL
                     DVSQFPDYLKEGLKMSKIIAYLFLFFFVFYSVSVFLKSRYKEFGILYITGISKRQVMK
                     LIFIENMIINIVSSVIGIIIGLIFAKIFLVVMSTFLELSPLKFYIPFNAILSTLIYFM
                     ILAVLTSIFTSFIVKENQVLKLLKGTKTPKPEPKTSLMLAILSICLFIIAYYNAITST
                     DQYTTYLRLVPVTSLTIVATYLFFSQFSVFFMKKLKLNKRFYRKNTNMLWISNLIYKV
                     KDNARIFFLITITSAVAFTAIGTVYSFWKDVERQINLIYPNTIYYSTMTLHNDPKKPD
                     SKEKDKERMMFIENNLKKENVDYTRINGEFKTVFPKKSDFNVRIMKESKYLEITETIG
                     VKPISFEDNECISLLSSSLPGNKNVKENIIVGNRGLKVAKQVEECVMPAYYKNMYVVK
                     DNFYDSIESKFIVDRFTAFEVEDSSKTIEICREFENKFNNESGMQPYLFFSKELMIGT
                     GKLMYSTFIFLAIFIGLIFFVTTSSFLYNKFYMDCQVDKKKYEQLNKLGMTYNEIKKA
                     STIEIGIVFLFPYVVAVIHSIFALTALKNSFDIEVASSAVLVMGSLFLVQIVYFLIVR
                     NNYLKEIRLNLVNF"
     gene            13969..14886
                     /locus_tag="KR250_002639"
     CDS             13969..14886
                     /locus_tag="KR250_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HBH0068561.1"
                     /translation="MMILIQSRGKMKCKELSEELEVSERQIKSYKMYLEQAGIFINST
                     PGIYGGYEIDKCNSISLIKLLDSEVSILDMINSQLEYNNDIYKNEFNNIVEKIKAVLN
                     TGEKSDTYMDYFTVQAQRNCDYESEKNKCNEIIRAYTTKHKFWIEYYSLNSGNSERIV
                     HPYGLFNYKSDTYMVAFCEKRFKFIDFKLCRIKDYKVLEEKYNVDKSFSWDEYSKNSI
                     GIYKGEEINVVIKISHPFSTIIKEKVWVNNQQIIEYDDKSIMFKAKMRGYEEIKSWIL
                     SMGAYVEVVEPDRLRNDILLEIEKMKKIY"
     gene            14964..15701
                     /gene="cas6"
                     /locus_tag="KR250_002640"
     CDS             14964..15701
                     /gene="cas6"
                     /locus_tag="KR250_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HBH0068562.1"
                     /translation="MKMKIEFSTGCIPISYNSLFMSIIKEAIRKSNEDYYKNMYYYKE
                     KNNKKTKNFTFSVYIKKYSIEGDNFIIEDKVILNISTPDLELGFHIYNGLMTSKKCLY
                     KDYELTRIRIDLSREKKVTEERVLFNALSPICVKSKEGKFLEITDDRYIEELNYITNE
                     VVKNYRGNGLKRKLEFENIGLKKVVVKESLREFKKITGKEYQYINGYKGKFILKGDID
                     DLNLIYNLGIGFRRAQGFGDVDILEWR"
     gene            15707..17557
                     /gene="cas8a1"
                     /locus_tag="KR250_002641"
     CDS             15707..17557
                     /gene="cas8a1"
                     /locus_tag="KR250_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein Cas8b1/Cst1"
                     /protein_id="HBH0068563.1"
                     /translation="MKLRVYRGDWFFNMGIVGFLNIIKKADKQAEIFIMEDYIEFDSL
                     FLENFHEYYFNYFMDEYDVSKRIKKNIDYSINFIKSKPDRIKDGIKKIKDSVKQQNDK
                     IKKFDEEKFNMIKEKLDSMSKIKSYDEFDSLEALVDETIDIFKIKSINDRLTANLYKY
                     VVQDNYFGQVSFFNVAKAKLDLDGLKQVMFNDYLRQIIYFGELEDLLEENDYDKLKNY
                     LNDRLNSIAKDINEKKVSKSSINTIEKIMKEINKNFIKKNKSIEDIKKYMDSLEVCEM
                     CGLYKGILDEYSESNFAPLGVSTNNARNMFWNQAYVPSICDICKLILFCTPAGATYTR
                     KNYLINEENEFYLFVNMDTCINELFERNNSLKAQKSEYSDSKDENPFNQLIKSIVEEN
                     TLKSEWQLRNILFVEFKASIDSKKCKINYFNIPTYLAKFFVKENKKIQSIYDYKLKSN
                     VLDLILKSKDLKHLINNTLRNIVKSNLESNNKSNISGLSCFNVVQLRTILNNYKRGVY
                     KMDYKKLRKIYESGCEIHDYYIDKNAKNKIEGITYKILNLIKVGNKNDFMQTILRIFS
                     PSEKTPPEEILQIFVEEDLDFESVGYAFVTGLISGKYEGKDKLPNEKEEK"
     gene            17559..18473
                     /gene="cas7i"
                     /locus_tag="KR250_002642"
     CDS             17559..18473
                     /gene="cas7i"
                     /locus_tag="KR250_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein
                     Cas7/Cst2/DevR"
                     /protein_id="HBH0068564.1"
                     /translation="MKRGLTFTVIAQAQSLNYGEGIGNISELKKLSRDDGNIYTFASR
                     QCLRYDIVRLAAELFNWNLQVVDKEKGTIQFKDNISIRESEEMDLFGYMKTNKKDEKD
                     KKGGSLTREATVRLSNAISLEPYRSDMDFLNNKGLADRIGEHPNLANIEQHLSYYTYT
                     VTIDLSKIGKDGDIELDNKEKCRRVVEFLEIIKVLNRNIRGRQENLSPLFVVGGVYEI
                     ANPFFLGRIKLKGDKNGFKINKQAIEEVIQGTFLGKDLKEFTYVGMVDGVFINKEEFK
                     GLFEDNFLSVDKFFGQLVKEVKEYYGVN"
     gene            18475..19176
                     /gene="cas5b"
                     /locus_tag="KR250_002643"
     CDS             18475..19176
                     /gene="cas5b"
                     /locus_tag="KR250_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein Cas5"
                     /protein_id="HBH0068565.1"
                     /translation="MKVLKLDLFQETACYKKPFAMKITETYPLPPYSTINGLIHKILN
                     ATEYIPFNISVQGTYESIFNNYQTTYFYKKDSITSMPMNSHMLLNVNLIIHIGGDFDL
                     LEEIYDSLLNYDEHLSLGRKEDLVRINDIRFVEVNKFEVDEDIQLDDYENYKLSECNI
                     RMPIYIPKTSLQDTDIDGISYRLNNYYDNNAVEDKKRVWNKVDSYYVEDGNTISIGDI
                     LLDNEGDLLYFNNIN"
     gene            19187..21508
                     /locus_tag="KR250_002644"
     CDS             19187..21508
                     /locus_tag="KR250_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HBH0068566.1"
                     /translation="MLYAKSNPVETLREHTDELLKQMNVLRESYGKNINSLDFLEEEI
                     FWQLLDFVIEFHDIGKAFSPFQELIKSRMDTKINEPKIVTYLENNVGHNYLSPAFIDY
                     SYIDRKKNKELRAVLNQVIVYHHERDIFIDKDFKNLIQKILDEDLINKVYELQHEFKV
                     RYPIKTEKLSKVYLQSVEKRIDKNHKYYNLYIMLKGILHRLDHSASAHEVVECNNVIN
                     IGEQTENYLMKEFGSLREAQSFAKSNRNKNIILIASTGMGKTETALIWIDKDKAFFTL
                     PLRVSINALFDRAKNIVGVGESNDTFLGLLHSTAIDYLEESNQENSSEIVDLAKLLSC
                     KLTFSTIDQIFKFPFLYRGYEKVYSTLAYSKVVIDEIQAYSPEIAAVLVKGIEMIHKI
                     GGRFMIMTATMPTIYIDELKKRGVMNSNLAELTCNTEKIRHCVSIVENSIDENLGKII
                     QSGMNKKVLVIVNTVKSAVEKYELIEEIVKPKGIDVNLNLLHSMYIQEDRAKLEKYIK
                     EFANSDRNGIWITTQLVEASLDIDFDELHTENSTLDSLFQRFGRCYRSREYEENSPNI
                     FIYTEKATGIGSIYDKDIVEKGLELLKTFINGKECVKMKEKYKVEMVKILYSKESLEG
                     TAFEKRFTSALNILDTITPYSLGSKDAQNILRNIEGYTVIPEDIYNKIEETLIKDYEE
                     LGQELINAYSNDDRQLINKIKSKRKKARREIIKKTVNLPIYKAKQNVIDIQVKGLEDL
                     KILLYKYDIYENKHTKQIFGKGVLISNEIDQFI"
     gene            21523..22074
                     /gene="cas4"
                     /locus_tag="KR250_002645"
     CDS             21523..22074
                     /gene="cas4"
                     /locus_tag="KR250_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HBH0068567.1"
                     /translation="MWLKFTKEGDFVPIDLEVLKVQGVKINYYYVCKRKLWLFSKGIT
                     MEQNSSRVLSGKIVHEDSYKRMKNKEILIDDILKLDIIKDEYVKEVKISSKMKEADEM
                     QLYYYLYYLKNSMGIEKKGTINYVKEKKQEELILTEEKERIIENTLININEINMQLSP
                     PKIEKKHYCTKCAYYEFCFVKEE"
     gene            22077..23057
                     /gene="cas1b"
                     /locus_tag="KR250_002646"
     CDS             22077..23057
                     /gene="cas1b"
                     /locus_tag="KR250_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated endonuclease Cas1"
                     /protein_id="HBH0068568.1"
                     /translation="MYKDYYIISNGDIKRKENTIYYYNLEGEKKILPVEQVDDIHIYG
                     EVDLNTKLINYISKYGVMLHFYNYYGYYSGTYYPRKKNVSGFSLVCQSACYLDKNERL
                     YLAKSFVESASYHILRNMRYYKVDESLIDTIKDELNDLNDVKEISELMGKEGRIRKTY
                     YKSFNQILKDDFEFYKREKRPPKDPVNALMSFGNSIMYTNVLSEIYKTQLDPTISFLH
                     EPSTKRFSLSLDIAEIFKPIIIDTIIFSLINNKRINKNDFEYQDKICYLSEKGKKKFL
                     QEFENKMQTTIKHRNLNRKVSYRMFIRLECYKLIKHFINDERYKVLKVWW"
     gene            23060..23326
                     /gene="cas2"
                     /locus_tag="KR250_002647"
     CDS             23060..23326
                     /gene="cas2"
                     /locus_tag="KR250_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HBH0068569.1"
                     /translation="MFVIVTYDIVEARSLNRIRRILRKYLTWTQNSVFEGNITEGKLH
                     KCISEIENIIDNSEDSIYVYEIKNPNSIKKKCYGIDKYSDEMFI"
     repeat_region   23510..25303
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=23510..23538
                     /rpt_unit_seq="gttttatattaactaagtggtatgtaaat"
     gene            25364..25792
                     /locus_tag="KR250_002648"
     CDS             25364..25792
                     /locus_tag="KR250_002648"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBH0068570.1"
                     /translation="MIKKLNNKDINKIMEIWEKSTIKAHDFISKEYWQNNYNTVKNEY
                     IPISDTFVYDDGDEIKGFISIIDKSFIGALFIKPKYQNLGIGGKLLDYATKKYKSLSL
                     AVYKDNKKAVVFYNKKGFNIVKEQVNEDSGFKEYIMEYSK"
BASE COUNT        10624 a         2358 c         4505 g         8660 t
ORIGIN      
        1 atatatcaga atatattttt aagctattta gagataattt aaatttttaa acaggacata
       61 ataagcttgt attgttttat atttgagttg ataaaaattt ctttaaataa aaataatgtc
      121 aattataaag taataaaagt ttagatataa aaaaatttgt gtatatacta tatgtagtat
      181 ataatagata aagcactgac tatatgtagt gcttttgttt ttgaaaaata cttattgcat
      241 ttctataatt atagattaat tttgggattt caactgataa gtaccaaaag aaataataag
      301 caaattaagt gaaattatgg cacaagatta tgttaaaagg acggtaaata atgaattgga
      361 tagagcttaa caatcaagtg ataattaaaa atgaacaagg caagtaccaa ttggaaaagg
      421 ataaggaagc cttaagtagt tacatagaag aatttataca gccaaagttg agaaaattta
      481 ataacctaga agaaagattg aagtatttaa tagatgaagg ttattattca aaagaagtaa
      541 taaatcagta tagcatagat gttattaaaa atatatatga tatgattgat aaacataagt
      601 ttgaatttca atcatatatg agtgcaaata agttttatca aaattatgca ttaaagagca
      661 atgatggaaa ggaaatatta gaaacatata atgataaagt gttaattgta gctttgacac
      721 taggaaatgg tgataaagat ttagctttaa acttggcaga taaaatagta aaacaagaat
      781 ttcaaccagc tacacctact tttttaaatg ctggaagaaa aagggctgga gaaatggtat
      841 catgtttttt actatctgta gaagatagta cagaaggtat atcatatgct atatcttcat
      901 caaatcactt atcaaagatt ggtggaggag ttgcactaaa tttatctaaa cttagagcat
      961 ctggcgaatc tatcaaagat atagaaggtg cagctggagg tgttgttgga gtagctaaaa
     1021 tgttagaaca atcatttagt tactttaatc aaatgggagc tagacaaggt tctggtgcgg
     1081 tgtaccttac tgtattccat cctgattttg aattgttgat ggatactaaa aagataaatg
     1141 cagatgaaaa aataaggctt gctacattat cattaggagc tataatacca gataaattta
     1201 tggagttggc agaaaaaaat gaagtagcat atgcatttta tcctcatact gtgtataaga
     1261 aatatggagt ttctcttgat gagatagaaa tggatgagtg gtatgataaa ttggttaatg
     1321 atagtgatat aagaaaaaaa gaaataaatc ctaggcagat gttgacaaaa atagcacaga
     1381 tgcaacaaga aagtggatat ccatatgtag tgtacataga tacagctaat agagaacata
     1441 cattaaaaga tgttggaatt attaaaatgt ctaacttatg ttgtgagata tttcaatatc
     1501 aaacaccttc agaaatagag gggtatggag gaaaaaatga atggggacaa gatataagtt
     1561 gtaacctagg ttctttaaac atagctaatg taatggataa taaaactata gaaagtacag
     1621 ttgaaacagc aataagagca ctgtcatttg tagcagatag cacagatata aaaccagtgc
     1681 caacggtaag caatagtaac agcaagtctc attctatagg attaggagct atgaatttac
     1741 atggatactt agtaagagaa aatattttat atacttcaga tgatgctata gatttttcta
     1801 atgtattttt tgcaatggtt agatactatt caattaaagc ttctatgaaa atagctatag
     1861 aaagaaatca aacttttgaa ggatttgata aatcagaata tgttaaggga agaaatagca
     1921 aggttttatc aaaatactat gaacaatctt atctacctaa gtcagaaaag gtaagagcat
     1981 tatttgaagg gatttatata ccaacaaaag aagactggac aaaattattg gatgaggtaa
     2041 aagaaaaagg gatatataat gcatatttaa tggcagtagc accaactcaa agcattagtt
     2101 atgttcaaaa tgctacatca agtataatgc caattactga gccagtagaa gtaagaacat
     2161 atggagactc tactacaata tatccaatgc catttttaac aaatgataat atgttgtatt
     2221 atcaatcagc atacaggatg gatatgagaa aggtcataga tttagtggca acagttcaaa
     2281 accatgttga ccaaggtata tcaacaacac tgtttgttac agatgaaaag acaactagag
     2341 atatagctag acattacata tatgcctata aaaaaggttt gaaaagttta tattacacaa
     2401 gaacaaagat gacaagagat actcatgagt gtctagtgtg ttcagtatag ggagagaaat
     2461 tatgacattt aatttacata aaatacataa tgcagttaac tggaatagag aagaagatgg
     2521 atttacacaa gctttttggg aacaaaatgt aaaacaattt tggcttcctg aagaaatatc
     2581 tgtttctaaa gacataaagg tatggaatga actaagtaat aaagaaaagg aattatataa
     2641 aaaagtattg ggtggtttaa cattactgga tacaaaacag gggaataatg gaataccatc
     2701 tatgatgagt ttaacagaaa acctacaaag aaaagctgtc ttgtctttta tggggactat
     2761 ggaagaaata catgcaaaaa gttattcttc tatatttatg actctacttt ctaatttaga
     2821 aatagatgaa ttatttgaat ggatagaaac agaaccaaca ttacaaagaa aggctgattt
     2881 agttttagct caatacgaaa atactacaaa tcaagaggga ttatacctat caatggttac
     2941 aagtgtattt ttagagagtt tcttgtttta ttcaggattc ttttatccat tgtatctatc
     3001 tggtcaagga aagatggtag ctagtggaga aataatatcg cttattctta gagatgagtc
     3061 tttacatggt aagtatatag gattgctagc acaagaaata tacgattcat ttgataaaat
     3121 ggataaggag atgttagaag agaagatgta ttctatactt tatagcctta tggagaatga
     3181 gatagagtat acaaatgtta tatacagaga aagtgggctt gaaaaagagg ttgtgaactt
     3241 tttaaaatac aatgcaaata gagctttaga aaatctaggg tttgagaagc tatatactgt
     3301 agatgctata aatccaattg tattaaatgg tcttagcact gaaaccaaaa cacatgattt
     3361 tttttcaact aagggtaatg gataccaaaa aggagtgtat gaggaattag aggatgaaga
     3421 ttttataata taatttataa ttatgacaca ctgttgtcac ttttagcgtg ataatatcaa
     3481 tgtataaaat atatatggag gtatttttat gaaatatgaa tggagaaaaa gagataaaga
     3541 gttatattta cctaaaaaga atcctgtaat agttgaaata ccagaacaga aatttatcat
     3601 gttaaaaggg agtggagacc caaattctaa agaatttact gaagcaatag gagtacttta
     3661 ttctttggca tatgcaataa aaaccatgcc taaaaaagga attgtaccag aagggtattt
     3721 tgactatacg gtttttcctt tagaaggtat atgggataag ggagaaaaaa gtaaaggctt
     3781 agatgtactt attaaagaag atttaattta cactataatg atacgtcagc cagattttgt
     3841 tacacaagac ctgttggata aagctactga tatagtaaag aagaaaaaac ctcataatct
     3901 tcttgaaaaa gttagatttg ggtctattaa tgaagggcta tgtgttcaaa tgcttcatat
     3961 aggttcatat gataatgaac cagaaagctt tgacattatg aacgaatttt gcaaaaaaaa
     4021 taaacttata agaaaatctt acacacatcg tgaaatttat ataagtgatg ctagaaaagt
     4081 atcgcctgaa tcattgaaga cggttttacg ttttgaagta gaaagggaag tataaatatt
     4141 ttgaataatt tataagagtt ataaaaagtg aagtctgttg agacttcatt ttttgatgca
     4201 attttaaaat taaatttgat gtttcataat attttaaaaa ctttctactt ttattagtgt
     4261 gaataactgt tgctattaaa taataatact aattatataa gtattattat ataatattta
     4321 tagattatta tataaataga ttattagtaa gatgtatgca ttattaatta aaataaatta
     4381 catatactat ttaatatttc atttaaaata ttaaaaaatg tagataaaaa ttgttttatt
     4441 aagaaaaaat taatatattg acaaaattta agtgctcatt taaactatta attgataata
     4501 atattcaata ttaaataata ggaggcgtta tgaataaata tcttctaata aatccagtgg
     4561 cagaaagaat gtatggagaa aagtttcacc ttattaaagc aaatttgata aaaaaggggt
     4621 acataataac agaatgtgaa ccacagttag aatatgtaaa aaagcagtac aaagagtata
     4681 caaaaaagac tgaaaataca atgttggatt gtagatgtcc agaaagtata agtcttttaa
     4741 aaagaaataa tttgataaat aattttgaag tacctatgat agaaccaata ttagtaagga
     4801 cttgtagggt tttatacgat aaatatataa aaagtaaaga tgatatgttg gtagtaacct
     4861 gtccatgtac acaattaaga gactttgcaa gtgaaaagtt taaggataag ggtaatgcta
     4921 tgttttatac atggaaagag tttgctgaac aagaaggtgt aaaatcactt ggaaaaatag
     4981 atgaatcacc aattccatta ggatatttca aagatacttt tgaaaaagtt cttgaagtat
     5041 caagtgaaga cgaaatatta agtgaaatac aaaaaataag aaatggtttt gaagataagt
     5101 atgacatagt agaaatgttt tattgtaaag atggttgtaa taatggagat ggtttgtaaa
     5161 tggtcaataa atttaataat aagtttttaa ggctagtaaa gagtgttctg atatttttta
     5221 taattctttt attatggaaa attacaaatt atcttggtat ttggagtgat tatatattac
     5281 catcacctga aaaggtatat agtacatttt taaatatgat aagtgatggt tcaatattta
     5341 ttaatgtata tgcaagtatg aaaagagttt tgataggttt tgcaataagt actgctatag
     5401 gtgtgccttt agggatattc tttggaattt atagtggagt atacgaatac tttaaatctc
     5461 taataaattt tttaaggaat acacctcccc ttgctttaat accaatgctt atattatggt
     5521 ttggtatagg agaggaatca aaaattataa ttatagtttt agcatctttt ttcccaatct
     5581 ttacaagcac attgaaaggg attaaaaatt gtgactcaaa actgattgaa gtgggtagag
     5641 tatttgagtt ttcaaagctt cagataatat ttaaaataat aataccaaat gccattcttg
     5701 atattgctgt tgggttaaag ttagctcttg gatatagctt tagagcaatt attggggcag
     5761 aacttgtagc agcttcatct ggtcttggat acttgatatc tgatggaaaa gaaatgtcta
     5821 gaacagatgt tgttattgta gggataatcg taattggact tttaggaata attacagatt
     5881 acatattttc gattattgtg aagaaggtaa gtaagggaaa gatggtggaa gcatatgaat
     5941 aatttagtat gtgactctgg atatataatt agaaatttat ataaaagtta taaagttgac
     6001 aataaagaac atgtagttct tagtgatatc tctttaaaca tagatagaaa ccatataaca
     6061 gtaattttag gtgaaagtgg ttgtggaaag actaccttgc ttagagcaat tgcatcactt
     6121 gaaggtataa atagtggtga aattaggttt attaatgata ataaagagta tagacctaat
     6181 gttggttttg tttttcagga gagtagatta atgccttggt taaacgtaag tgaaaatata
     6241 gcttttcata atcaaagaaa aaatactatt ttaaacaagg tgcttagaaa atttaaacac
     6301 aataaaagaa ttaggagtaa aattataaat gataatttta gaagcttaga aacaaatgat
     6361 aatttgaaca aatcaaataa atataatagg aaaagtaata ttgatgtaga aaaatatctt
     6421 aagatgatga acttgcagaa atttaaaaac tcttatccaa atgaattgtc aggaggtatg
     6481 gcgcagagag taagcatagc tagagctctt tctttcaatc cagatatgtt gcttatggac
     6541 gagccttttt cggcattaga ttattttaca aggattgata tgcaaaatga agttataaga
     6601 atacataagt caactaataa aggcgtgata tttgtaactc atgatattga tgaagcgtta
     6661 aagataggca agaaaatagt tgttttcaca gacgaaagaa aagtaaaaga atttaatata
     6721 gaagatggtt ataatagaga tttaacttca gattactata ttaaattaaa aaaagatatt
     6781 ttaatgacaa tgaagtcaaa taaaggcatt taaagaaatt aataatattt taaggagtaa
     6841 agtaaatgaa attaaaaaag ctattgatag gtataacgat attaacactt gcaacagcat
     6901 ctttagctgg atgtgctaaa aaaaataatg gagaaaaact ttcagaaata aatttaactt
     6961 atgtaaaatc accattaaat gttccttcta taattcagaa acaggatgat ttatttggaa
     7021 aggaatttaa aaaggataat ataaaagtta acttccacga aattactaca ggtccagagc
     7081 aaacacaagc tttagctgct ggagaaatag acttcttaca tgcactaggt ggtacatcag
     7141 ctttaatagc tgcatctaat ggagtagagt taaagatatt aaacacttat agcaggtcac
     7201 caaaaggatt tatgattttg actaataata attcaataaa atcagcagca gatttagtag
     7261 gtaagaaggt ggcaggccca aaagggacta tattacatca ggtattgatt tcagcacttg
     7321 ataaagaagg attaagtatg gatgatgtgg aatttgttaa tatgggtatt ccagaggcat
     7381 cagcagctct ttctgatggc agcgtagatg ctgcattgat tgctgggcca gcagctctta
     7441 aagctatgaa gtcaggtagt aaacttgtag caaatggtga aggtttagta gatggtataa
     7501 tagtaactgc tgttagcaca gactttgcag agaaacatcc agaaatagtt gaaagattta
     7561 tgaaggttga aaaggaaact ttagaatatg taaataataa ttttgatgaa gctatggaaa
     7621 aagtagctaa agaagttgat ttaagtcttg aagaaactaa agaattatat gcttggtatg
     7681 attttagttt agatataaca gataaagaca tatcttcgct tgaagataca caagacttct
     7741 taataaaaaa taagttacaa gagaaaaaag tgaacataaa agaattaatc tataatgcta
     7801 agtagaaact atgataaaat aatactaagt aagaaatata ataaaagttt aagttacctt
     7861 attaaggtaa ctttttctat tttgaattag taatttcaat ttattttaga atgattagtt
     7921 gataataatg attaattaat cataaaaaga tactttttta tagagtttta ttaagatgtc
     7981 taataatatg gttatactta tgaagtcagt aattgtgaag ttagtaatat gattaaattt
     8041 atatattgta aaaagattat aatatattaa aattggactg ttatatgatg agtaaatagg
     8101 tatattagga ggaattatat gagttataaa gtatatattg tggaagatga catatcaata
     8161 agtacattac ttaaagatta tattgagaaa tatggatttg aagtgattgt tgaagagaac
     8221 tttgatgata taatgattac atttgaaagt tttaagccac acttagttct attagatgta
     8281 aatttaccta aatttgatgg tttctactgg tgtagatgta ttaggcaaca gtcgaattta
     8341 cctataattt ttatatcagc tagagatggt aatgtagacc aaattagagc tttagaaagt
     8401 ggagcagatg attatataac aaaacctttt tactatgatg tagtaatggc aaaaataaag
     8461 agtcatataa gaagggtata tggtgaatat tcacctaaaa tagacgaaag aatagtggag
     8521 ctagatggat taaaatttta tccagaaagg ttagaactag aattaaatgg tgagaatcta
     8581 attataacta aaaaagaagg tatactgatt gaatgtctta tcaagagata cccaaaagta
     8641 gtaagtagag attatctttt agaaaagata tgggatgata ttgagtttgt agaggaaaat
     8701 actttaaatg taaatgttag caggataagg aaaagatttg aggaattagg tatagataat
     8761 gctgttcaga ctgtccgagg gttaggatat aaattaaata agaattggta aggagaaagt
     8821 atgaagttat ttttaaaaga ttataaaggt tatattttta tatattatat aggcatagtt
     8881 atgacaatta tttactgtaa tcttatgaag tttattagtt ttggagagag tttatatatt
     8941 cttttattta gttcttttat attgttatgt tttttaatat ataagtatta taaaaataga
     9001 gaagtatata atctgtttga aaaaggagta cattctttgg aggagtcatt tttgtattta
     9061 ggaagctcat tcttggggag aaatatatca gttattttaa aacaacaaca taaattgtat
     9121 caatcaatga tacaagagca taaaaagata catagtgaac atttaacttt catcaaccaa
     9181 tggattcatc agatgaaaac accaatatca gttataagcc ttcagctcca agattatgag
     9241 ggagaagaag taactgaaaa tataagaaaa gaaatagata gattgaataa agggttagat
     9301 atggctatgc attttgcaag ggcagataat tttcagaaag actttatagt tgaaaaagcc
     9361 aaattaaatg aaattgtatc tagtattgta aatgaagaaa aaaggttatt tataaaaagt
     9421 ggaatgattc ctaagattca agtagacaag tttacttatg tttatacaga tgtaaaatgg
     9481 atgaaattta taataggtca attaattaca aatggagtga aatattctaa agattatagt
     9541 aactacctaa ctataaaatc tgaagaaaat aataaatatg taattttaag tataatagat
     9601 gaaggaattg gaattgtttc taaagattta aaaagggtat ttgccccatt ttttacagga
     9661 gaaaatggtc gtaagtttgg agaatctaca ggtatgggtc tttatctagt aaaaaaggta
     9721 tgtgatgatt taggtcataa agtaagtata aaatcagaag taggaaaagg tacagaagtt
     9781 tcaatctact ttaataaagg tgtaaatagt ttgataaaga tgtaaattat ctgttacaat
     9841 agggttttta gttaattgta cttttttatt atacaattta ttaaatgaaa tattcatagt
     9901 taaataatca aaatttaaga gagtttattt gcaaacctta catagatgta aggtttgtgt
     9961 aatgtttgca gatagttaaa ttttctaatc taaattacaa ttaatataag aaataacata
    10021 agagaaataa cataaaaaaa gaaattttgt aaaaaaggaa ggatgtttat tgatgagttt
    10081 agatgttatt tttatattga aatcagtcat aatagctata gtagaggggc ttacagaatt
    10141 tattccagtt tcatctacag gtcatatgat tttggttggt aatttaatag attttaaggg
    10201 gcaatttgct gaaatgtttg aggttgtaat acaacttggg gctatattag cagtagtagt
    10261 actttattgg aaaaagataa aagatagcgt tatagaattt tttaaattta tatttactgg
    10321 tggaaaagaa ggaaagattg gttttagatt tggaatgaat gtaattatag gttgtatacc
    10381 atttgcgatt ataggagttc tattttacga taatataaaa tcattgttta atttacaatc
    10441 tgtaattata ggatttatag tgggtgggat tttattatta gtagtagaaa ctttgtttag
    10501 aaaaaagaat cattccacag ataatataga taaaattact ccaatacaag ctttaaaggt
    10561 tgggacatta caagttttgt ctgcatggcc aggtatgtca aggagtgcat ctactattat
    10621 gggtggatgg atagcaggac ttaattctcc tacagcagct gagttttctt tctttttagc
    10681 agtaccagct atggtggctt cttcaggaaa agatttattt gaatttgatt attcaataat
    10741 gacaccaaca ttgtggattg ctttggtagt tgggtttata gtagctttta ttgtatcaat
    10801 aatagttatg gaaaaattcg tgaatttttt aaagaaaaaa ccaatgagag tgtttgctgt
    10861 atacagaata atcatgggtg tagtacttgc agtattagct tttactaaca taattagtgt
    10921 atagaaaaaa tagatttatt atctataaaa attaaactaa gataaattat aattaacaat
    10981 tatatatatg ttatttcaaa aattaaatta ggaaggatag ttaaataatg aaaattttaa
    11041 ctgtaaataa tttaagtaaa gtatatggta aaaaaataat tttcaacgca ttaaatgaca
    11101 taaattttag tatagaagat ggagaatttg taggaattat gggtccatct ggtagtggaa
    11161 aaactactct tttaaatatg atttctacta ttgacaagcc aactacagga actatggaat
    11221 taaagggcaa gaatccttta ttgttaagag gtgaggaact tgcattattt agaagaagag
    11281 agttaggttt tgtatttcag gattttaatt tattggatac attaactata ggagaaaata
    11341 tagtattgcc attgacatta gataaggtca gtgtaaaaga gcaagatgag agattgaatg
    11401 aagtatctac aatattaggt ataaaagatt tattaggaaa aagaactttt gaagtatcag
    11461 gtggtcaagc tcagaggact gctattgcta gagcattgat taacaatcca tcaatacttt
    11521 tagcagatga accaacaggt aatttagatt caaagtcatc aaaggttgta atggagctat
    11581 ttcaaaagat aaataaagaa aataaagtta ctactatgat ggtaactcat gacccattag
    11641 cagcgagcta ctgtagtaga atcttattca taaaagatgg gtctatatat aatgaaatat
    11701 ataagggtag tagtagagag cagttttatc aagagataat ggatgtactt acattattgg
    11761 gaggcgataa ctaatgaata taaaacagtt tgctattaac aatatttcaa gaaatattaa
    11821 agcgtattta ggatacttag caagtattgt tatatcatct tcactgctat tttcttttat
    11881 tatgtttaca agtcatccag atttagatgt atctcaattt cctgattatt taaaagaagg
    11941 tctcaagatg agtaagataa ttgcatatct attcttattc ttctttgtgt tttattcagt
    12001 tagtgtgttt cttaaaagta gatataaaga atttggaata ctttatatta caggtatatc
    12061 aaagcgccaa gtaatgaaat taatctttat agaaaatatg ataataaata tagtatcttc
    12121 tgtaattgga ataataatag gcttaatctt tgcaaagata tttttagtgg ttatgtctac
    12181 ttttttagaa ttatcaccat taaaattcta tataccattt aatgcgatat tgtctacttt
    12241 aatctatttt atgattttag ctgtattaac atcaatattt acttctttta tagttaaaga
    12301 aaatcaggtg ttgaagttac ttaaaggtac aaaaactcca aaaccagagc ctaaaacatc
    12361 actaatgctt gctatattaa gtatttgttt gtttataata gcgtattata atgctattac
    12421 ctcaaccgac caatatacaa catatctgcg cttagttcca gttacatccc ttactatagt
    12481 agcaacttac ttgttctttt ctcaatttag tgtatttttt atgaagaagc taaaattaaa
    12541 caagagattt tatagaaaaa atacaaatat gctttggatt tcgaacttga tttataaggt
    12601 aaaggataat gctagaatat ttttcttgat aactataact tctgcagtgg catttactgc
    12661 tataggtaca gtttattcct tttggaaaga tgtagagcgt caaattaatt taatatatcc
    12721 taacacaata tattattcaa caatgacatt gcataatgac cctaaaaagc ctgatagcaa
    12781 agaaaaagac aaggaaagaa tgatgtttat agaaaataat ctaaaaaaag agaatgtaga
    12841 ctatacaagg attaatggtg aatttaaaac tgtatttccc aagaaaagtg attttaatgt
    12901 cagaattatg aaagaatcta agtacttaga aattacagaa actatagggg ttaagcctat
    12961 aagttttgaa gataatgaat gtatatcatt attatcatca agtttaccag gaaataagaa
    13021 cgtcaaagaa aatattattg tagggaatag aggtttaaag gtggcaaaac aagtggaaga
    13081 atgtgttatg ccagcgtatt acaaaaatat gtatgtggta aaggataatt tttatgatag
    13141 tatagaatca aaatttatag tggatagatt tactgcattt gaagtagagg attcaagtaa
    13201 aactatagag atatgcagag aatttgaaaa taaatttaat aatgagtcag ggatgcaacc
    13261 atatttgttt ttctctaaag aattaatgat aggaactggg aaattaatgt attctacatt
    13321 tatattttta gcaatattta taggtttaat tttctttgtt actacaagta gttttttata
    13381 taataaattt tatatggatt gccaagttga taagaaaaag tatgaacagt taaataaatt
    13441 gggtatgaca tataatgaga ttaaaaaagc ttcaacaatt gagattggaa tagtattttt
    13501 gtttccttat gtagtggctg ttatacattc aatatttgca cttactgcac tgaaaaattc
    13561 ttttgatata gaagtagcat catcagctgt tttggtaatg ggaagcttgt tcttggttca
    13621 aatagtttat tttttaattg taagaaataa ttatttaaaa gagataaggt taaatttagt
    13681 caatttttag ttactattat taaatgataa taaaaagggt ttagaattaa ttaatattac
    13741 tggaattata gatgtttatt ttttcagtaa tattttttat tgtattaact attaaaattt
    13801 tgtacaaata taaaatataa tatggtaaat gtcaaaatta taaaaaaatt ctacttatat
    13861 aaaatataaa gaggttatat gtacatatgt gtagaaataa ataataaaaa tatcaattaa
    13921 tgttattagg gggggttact cattagtaaa ttagctaata cacttagaat gatgatatta
    13981 attcaaagca gaggcaaaat gaaatgcaaa gaattatcag aagagttgga agtaagtgaa
    14041 aggcaaataa aaagttacaa gatgtattta gagcaagctg gtatatttat aaattcaaca
    14101 cctgggattt atggtggtta tgaaatagat aaatgtaata gcatctcatt aattaaatta
    14161 ttagatagtg aagtttcaat tctagatatg ataaattcac agttagagta taataatgat
    14221 atttacaaaa atgaatttaa taatattgta gaaaaaataa aagcagtatt aaatacagga
    14281 gaaaaaagcg atacatatat ggattatttt acagttcaag cacaacgtaa ttgtgattat
    14341 gagtctgaaa aaaataagtg taatgaaata attagagcat ataccacaaa acacaaattt
    14401 tggatagaat attattcatt aaattcagga aatagtgaaa gaattgttca tccatatggt
    14461 ttatttaatt ataaatcaga tacttatatg gtagcttttt gtgaaaaaag atttaagttt
    14521 atagacttta aattatgtag gattaaagat tataaggttt tagaagaaaa atataatgta
    14581 gataaaagtt ttagttggga tgaatatagt aaaaatagta taggaattta taagggagaa
    14641 gaaattaatg ttgttataaa aattagtcat ccattttcaa caattataaa ggagaaagtt
    14701 tgggttaata atcaacaaat catagaatat gatgataaat ctataatgtt taaagctaaa
    14761 atgagaggtt atgaggagat aaaaagttgg attttaagta tgggagctta tgttgaggtt
    14821 gtagaaccag atagacttag aaatgatata ttgttagaga ttgaaaaaat gaaaaaaatt
    14881 tactgatttt ttcaaataat ttatacttgg accaatatac ttcacggtct agtgttaata
    14941 tattaattga gagaggtgat attatgaaaa tgaaaattga atttagtaca ggttgtatac
    15001 caatatctta taattcgctc tttatgagta ttataaaaga agcaataaga aaatctaatg
    15061 aagattacta taaaaacatg tactattata aagaaaagaa taataaaaaa actaaaaatt
    15121 ttacattttc ggtttatata aaaaagtata gtattgaagg tgacaatttt attatagaag
    15181 ataaagtaat attaaatata agtactccag atttagaatt gggttttcat atatacaatg
    15241 ggttaatgac ttctaaaaaa tgtttatata aagactacga acttacaaga attagaatcg
    15301 atttatcaag agaaaagaag gtaacagaag aaagggtact atttaatgca ttgtctccaa
    15361 tctgtgtaaa gtctaaagaa ggaaaattct tggagataac tgatgataga tacatagaag
    15421 aactaaacta tataactaat gaagtggtta aaaattatag ggggaatgga ttaaaaagaa
    15481 agttagaatt tgaaaatata ggtctcaaaa aggtagtagt taaagagagt ttaagagaat
    15541 ttaaaaaaat aacaggtaaa gagtatcaat atattaatgg ttacaaaggt aaatttatat
    15601 tgaaaggtga tattgatgat ttaaatctta tctacaatct aggaattggt tttagacgtg
    15661 ctcagggatt tggagatgta gatattttag aatggaggtg agataaatga agttaagagt
    15721 ttatagagga gattggtttt ttaatatggg tatagttgga tttttaaata taataaagaa
    15781 agcagataaa caagctgaaa tatttattat ggaagattat atcgaatttg atagcttatt
    15841 tcttgaaaac tttcatgagt attattttaa ttactttatg gatgaatatg atgtatcaaa
    15901 aagaattaaa aaaaatattg attacagtat aaattttatt aaatctaagc ctgatagaat
    15961 caaagatgga attaagaaaa ttaaagatag tgtgaaacaa caaaatgata agattaaaaa
    16021 atttgatgaa gaaaaattta acatgataaa agagaagcta gatagtatgt ctaagataaa
    16081 aagttatgat gaatttgata gtttagaagc tttagtagat gagactatag atatatttaa
    16141 aataaaatca ataaatgaca ggcttacagc aaatctttat aaatatgtag ttcaagataa
    16201 ttattttgga caagttagtt tttttaatgt tgctaaggcg aaattagatt tagatggatt
    16261 aaaacaggta atgtttaatg attatttaag acaaattata tattttggag agttagaaga
    16321 tttattagaa gagaatgact atgacaagtt gaaaaactat ttaaatgata gattaaatag
    16381 tatagctaag gatataaatg aaaaaaaagt tagtaagtct agtataaata ctatagaaaa
    16441 aattatgaag gaaataaata aaaactttat aaagaaaaat aaaagcatag aagatataaa
    16501 aaagtatatg gatagtttag aagtatgtga aatgtgtggt ttatataagg gaatattaga
    16561 tgaatactca gaaagtaatt ttgctccact tggagtaagc accaataatg ctaggaatat
    16621 gttttggaat caggcgtatg taccatcaat atgtgatatt tgtaagctaa tattgttctg
    16681 tactccagca ggggctactt atacaagaaa gaattattta attaatgaag aaaatgagtt
    16741 ttatttattt gttaacatgg atacttgtat aaatgagctt tttgaaagaa ataatagttt
    16801 aaaagctcaa aaaagtgagt attcagattc caaagatgaa aatcctttta atcagcttat
    16861 aaaaagtata gtagaagaaa atacattaaa aagtgaatgg caacttcgta atatactttt
    16921 tgtggaattt aaagcaagta tagattctaa aaaatgtaaa ataaattatt ttaatattcc
    16981 aacttattta gcaaagtttt ttgtaaaaga aaataaaaaa atacaaagta tatatgatta
    17041 taaacttaaa tcaaatgtgt tagatttaat tttaaaaagt aaagacttaa aacatttaat
    17101 taataacact ttaaggaata tagttaagag taatttagaa agcaataata aatcaaacat
    17161 atcagggtta tcttgtttca atgttgttca attaagaaca atattaaata actataagag
    17221 aggagtgtat aaaatggact ataaaaagtt gagaaagatt tatgagtcag gttgtgaaat
    17281 acatgattat tatattgata agaatgctaa aaataaaata gaaggaataa catataaaat
    17341 tttaaattta attaaggttg gtaataaaaa tgattttatg caaacaattt taagaatttt
    17401 ttcaccaagt gaaaaaacac caccagaaga aattttacag atatttgttg aagaagattt
    17461 agattttgaa tctgtagggt atgcatttgt aacaggatta atttctggaa aatatgaagg
    17521 aaaagataaa ctaccaaacg aaaaggagga aaaataagat gaaaagaggg ttaacattta
    17581 cagtaatagc acaggctcaa agtttaaact atggggaagg gataggtaat atatcagaat
    17641 taaaaaaatt aagtagagat gatggaaata tatatacatt tgcatctcgc cagtgtttaa
    17701 gatatgacat agtacgatta gcagcagaat tgtttaattg gaatttacag gttgtcgata
    17761 aagaaaaagg cactattcaa tttaaagaca atataagtat aagagaatca gaagaaatgg
    17821 atttatttgg atatatgaaa acaaataaaa aagatgagaa agataaaaaa ggaggttctt
    17881 tgactagaga agctactgta agattaagta atgcaatttc ccttgagcca tatagaagtg
    17941 acatggactt tttaaataat aaaggattag cagatagaat aggagaacat cctaatcttg
    18001 ctaacataga gcaacattta agttattata catatacagt gactatagat ttatctaaaa
    18061 taggtaagga tggagatata gaacttgata ataaagagaa atgtagaaga gtagtagaat
    18121 ttttagaaat aataaaagta ttgaatagaa atattagagg tagacaagaa aatttatctc
    18181 ctctatttgt agtaggagga gtatatgaaa tagcaaatcc atttttcttg ggtagaatta
    18241 aattaaaagg agataaaaat ggatttaaaa taaataagca agctatagaa gaagtaattc
    18301 aaggtacttt tttaggaaaa gatttaaaag aatttaccta tgttggaatg gtagatgggg
    18361 tatttataaa taaagaagaa tttaagggat tgtttgaaga taatttttta agtgtagata
    18421 aattttttgg acaactagtt aaagaagtaa aggaatatta tggggtgaat taatatgaag
    18481 gttttgaaat tagatttatt tcaagaaact gcatgctaca aaaaaccatt tgctatgaag
    18541 attacagaga catatccact gcctccatat tctacaataa atggattaat tcacaaaata
    18601 ttaaatgcta cagaatatat accatttaat ataagtgtac agggaacata tgaaagtata
    18661 tttaataatt accagacaac atatttttat aaaaaagata gtattacatc tatgccaatg
    18721 aatagtcata tgctattgaa tgtaaatttg ataattcata taggtgggga ttttgattta
    18781 ttggaagaaa tatatgatag tttattaaat tatgacgagc acttatcatt aggtagaaaa
    18841 gaagatttag taagaattaa tgatattaga tttgtagaag tcaataaatt tgaagtagat
    18901 gaggatatac aattagatga ttatgaaaat tataagttat cagaatgtaa tataagaatg
    18961 cctatatata taccaaaaac aagtttacaa gatacagata tagatggtat aagttacaga
    19021 ctcaataatt actatgataa taatgctgtg gaagataaaa aaagggtttg gaataaggta
    19081 gatagttatt atgttgagga tggaaatact atatcaattg gagatatatt attagataat
    19141 gaaggagatt tattatattt taataatata aattaggagg gagattatgt tatatgctaa
    19201 atctaatcca gtggaaacac ttagagaaca tactgatgaa cttttaaaac aaatgaatgt
    19261 attaagagaa agttatggaa aaaatataaa tagcttagat tttctagaag aggagatatt
    19321 ttggcaatta cttgactttg ttatagaatt tcatgatatt ggaaaagcat tttcaccgtt
    19381 tcaagagtta ataaaaagca gaatggatac taaaataaat gaacctaaaa tagtaactta
    19441 tttagaaaat aatgtaggcc ataattatct ttctcctgca tttattgatt atagttatat
    19501 agacaggaaa aaaaataaag aacttagagc agtattaaat caagttattg tatatcatca
    19561 tgaaagagat atctttatag ataaagattt taaaaattta atacaaaaaa tattagatga
    19621 agatttaata aataaagttt atgaactaca gcatgaattt aaagttagat atccaataaa
    19681 aacagaaaaa ttgagtaaag tatatctaca aagtgtagaa aaaagaatag ataaaaatca
    19741 caagtactat aatttatata ttatgttaaa aggaatacta catagactag accatagtgc
    19801 ttctgctcat gaagttgtgg aatgcaataa tgttataaat ataggagaac agacagaaaa
    19861 ttatttaatg aaagagtttg gtagtttgag agaggctcaa tcatttgcta aatctaatag
    19921 aaataagaat ataatactga ttgcatctac aggaatggga aaaacagaaa ctgccttaat
    19981 ttggatagat aaagacaagg cattttttac acttccatta agagtaagta taaatgcatt
    20041 atttgataga gcaaaaaata tagttggtgt tggagaatca aatgatacat tcttaggttt
    20101 gttacattca acagcaatag attatctaga agaaagtaat caagaaaatt caagtgagat
    20161 tgtagattta gcaaagttat tatcttgcaa actgacattt tctacaatag accaaatatt
    20221 taaatttcct tttttgtata gagggtatga aaaagtgtac tcaacattag catattcaaa
    20281 agtagtaata gatgagatac aagcatattc accagaaata gcagctgtat tggtaaaagg
    20341 tatagaaatg atacataaga taggtggcag atttatgata atgacagcta ctatgccaac
    20401 catatatata gatgaattaa aaaaaagagg tgtaatgaat agtaacttag ctgaattgac
    20461 atgtaataca gaaaaaataa gacattgtgt ttctattgta gaaaattcaa tagatgaaaa
    20521 cttaggtaaa ataatacaaa gtggaatgaa taaaaaagtt cttgtaatag taaatacagt
    20581 taaaagtgct gtagaaaaat atgagttaat agaagaaata gttaaaccaa aaggaataga
    20641 tgtcaactta aatcttttac attctatgta tatacaagaa gatagagcaa aattagaaaa
    20701 atatataaaa gaatttgcca atagtgatag aaatggaatt tggattacaa cacaattagt
    20761 agaggcatct cttgatattg attttgatga gcttcataca gaaaattcaa cacttgacag
    20821 tctatttcaa cgatttggta gatgttatag aagtcgtgag tatgaagaaa atagcccaaa
    20881 tatatttata tatacagaaa aagctacagg aataggtagt atttatgata aggatattgt
    20941 agaaaaagga cttgagctac tgaaaacttt tataaatgga aaagaatgtg tgaagatgaa
    21001 agaaaaatat aaggtagaaa tggttaagat actatactct aaagaaagtc tagaaggtac
    21061 agcttttgaa aagagattta ccagtgcttt aaatatttta gatactataa cgccatatag
    21121 tttaggttct aaagatgcac aaaatatact tagaaatatt gaaggatata cagtaattcc
    21181 agaggatata tataataaaa ttgaagaaac tttaatcaaa gattatgaag agttgggaca
    21241 agagcttata aatgcatata gtaatgatga tagacaattg ataaataaaa taaagtctaa
    21301 gagaaaaaaa gcaagaagag agataattaa aaaaactgta aatttaccaa tatataaagc
    21361 taaacaaaat gtaattgaca tacaggtgaa aggtcttgaa gatttaaaaa tacttttata
    21421 taaatatgat atatacgaaa ataagcatac aaaacagata tttggtaaag gtgtgcttat
    21481 aagtaatgaa atagaccaat ttatttgatt tttatattaa caatgtggct aaaatttact
    21541 aaagaaggtg attttgtgcc aatagattta gaagtattaa aagttcaagg tgtaaaaata
    21601 aattattatt atgtatgtaa aagaaaacta tggctatttt ctaaaggaat aaccatggaa
    21661 caaaatagta gtagagtatt gtctgggaag atagtacatg aagattcata taaaagaatg
    21721 aaaaataaag aaatattgat tgatgatatc ttgaagctag atataataaa ggatgagtat
    21781 gtaaaagaag taaaaataag ttcaaagatg aaagaagcag atgaaatgca gttatactat
    21841 tacttatact atttaaagaa tagtatgggt atagagaaaa aaggtactat aaattatgtt
    21901 aaggaaaaga aacaagaaga attgatattg acagaagaaa aagaaagaat tattgaaaac
    21961 acacttataa atataaatga aataaatatg cagttatctc ctcccaaaat tgagaaaaaa
    22021 cattattgta caaaatgtgc ttattatgaa ttttgttttg tgaaggagga gtaacaatgt
    22081 ataaagacta ttatattata agtaatgggg atataaaaag gaaagaaaat acgatttact
    22141 attacaattt agaaggtgag aaaaaaatat taccagtaga acaagttgat gatattcata
    22201 tatatggaga agtagattta aatacaaaac ttataaatta cataagcaaa tatggggtga
    22261 tgcttcattt ctacaattac tatggatatt attcaggtac atattatcca aggaagaaaa
    22321 atgtatcagg attcagttta gtatgtcaat cagcctgcta tttagataaa aatgaaagat
    22381 tatacttagc aaaatccttt gtggaaagtg catcatacca tattttaaga aatatgagat
    22441 attacaaagt tgatgaaagt ttaattgata ctataaagga tgaactaaat gatttgaatg
    22501 atgtcaaaga aatatcagaa cttatgggta aagaaggcag aataagaaaa acatactata
    22561 aatcatttaa tcaaatatta aaagatgatt ttgaatttta taaaagagaa aaaagacctc
    22621 ccaaagaccc agtaaatgct cttatgtctt ttggaaatag cataatgtat acgaatgttt
    22681 taagtgagat atataagaca caacttgacc ctacaataag ctttttacat gaaccatcaa
    22741 caaagagatt ttctttaagt ttagatattg cagaaatttt taagccaatt ataattgaca
    22801 caataatttt tagcctgatt aataataaaa gaattaataa gaatgatttt gaatatcaag
    22861 acaaaatttg ttatttaagt gaaaaaggaa agaagaaatt tttgcaagaa tttgaaaata
    22921 agatgcagac aactataaaa catagaaatc taaatagaaa agtttcatat agaatgttta
    22981 taaggttaga gtgttataaa ttaattaaac attttataaa tgatgaaagg tacaaagttt
    23041 taaaggtgtg gtggtaagta tgtttgttat tgttacttat gatattgttg aagcaaggtc
    23101 gttaaataga attagaagga tacttagaaa atatttgact tggacgcaaa attctgtttt
    23161 tgaaggcaat attactgaag gaaagttaca taaatgtatt tctgaaatag aaaatattat
    23221 tgataatagc gaggattcaa tctatgttta tgagataaaa aatcctaatt caattaaaaa
    23281 gaaatgttat gggattgata agtattctga tgaaatgttt atataggttt gcagtgagcg
    23341 atatttatgc taaaataggt gttaacagtt ggaatataag ggattgaagg tgtatgataa
    23401 ttgttatcaa ttgcactact gctcgctcac tgcaaatttt gatgttttta ttgaattata
    23461 attgcttgat tgaagtgttt tcaatgtatt caaatatacc tattttgggg ttttatatta
    23521 actaagtggt atgtaaataa gtgtggttca acatccttga cagctcaaaa aaaagtttta
    23581 tattaactaa gtggtatgta aataattttt tcggatattt aataatttat tttaaattta
    23641 gttttatatt aactaagtgg tatgtaaatc tcaacagtat ctacaagaac ttctatatct
    23701 tctaagtttt atattaacta agtggtatgt aaattaattt agggtaactt ttatattatt
    23761 ttcttctatc gttttatatt aactaagtgg tatgtaaatt tggttgatat agatattagt
    23821 cctattattt ctgcttgttt tatattaact aagtggtatg taaataaatt agataattta
    23881 taagtcaata aaataagtcc aagttttata ttaactaagt ggtatgtaaa taaatttgta
    23941 atattatata atcatcctaa aacaaaaggt tttatattaa ctaagtggta tgtaaattcg
    24001 aatgctataa ttttaatata taaaataaat ggagttttat attaactaag tggtatgtaa
    24061 atatggattt tagtcgtttt taaatttttc tcgtaatagt tttatattaa ctaagtggta
    24121 tgtaaatacc attcatttat atttttatct gtatctctta gccatgtttt atattaacta
    24181 agtggtatgt aaattaagtt ttcttaaaaa agtacttgct ttaaatatgt tttatattaa
    24241 ctaagtggta tgtaaatttt attttctctt tattagtcat tagtttttgc cttcgtttta
    24301 tattaactaa gtggtatgta aatatgaaga atttgcgaaa ctaataagaa aagatattga
    24361 gttttatatt aactaagtgg tatgtaaatc tttctttatc aaagtaagct aaatttagtt
    24421 cctccgtttt atattaacta agtggtatgt aaatcgtcat tactttttat tgcttccatt
    24481 aaatttgttc gttttatatt aactaagtgg tatgtaaata tcgcaagata ggctaaatct
    24541 atcaaggaga gttgagtttt atattaacta agtggtatgt aaatgactct gccatccctg
    24601 taacaaacag tcttaccgca agttttatat taactaagtg gtatgtaaat aaatatttta
    24661 cgattagttg atgctataat tctattagtt ttatattaac taagtggtat gtaaattctt
    24721 ttgatttagc gttgtttgtc cggaatttta aagttttata ttaactaagt ggtatgtaaa
    24781 ttcgggctag aatgtatttt aaaccctagt tcattgaagt tttatattaa ctaagtggta
    24841 tgtaaatttt ctaatatcgc tatttaaact caatgcttca tttgttttat attaactaag
    24901 tggtatgtaa attcagaaca aattaaatat gtttcgtttt ccatgtaagg ttttatatta
    24961 actaagtggt atgtaaatag aactttttta tattagaagt acttgacatg attgccgttt
    25021 tatattaact aagtggtatg taaatcttga taatatttca gcatatgata ctgaagatgg
    25081 ttttatatta actaagtggt atgtaaattt gccaatgtaa catctttaga tattacagta
    25141 tcattgtttt atattaacta agtggtagat aagtagaata ttagcaatat caacgagtat
    25201 ttagaaactt attttatatt aataaagcag tatataaaat tgtagaatca acaatagcat
    25261 atactaaaac atccacctta tattaactaa atcatatcta aataaaataa acttacctat
    25321 aaacattata aaatcaatac aaaaatgagg tgaaataaaa tttatgataa agaaattaaa
    25381 caataaagat ataaataaaa tcatggaaat atgggaaaaa agtacaatca aagcacatga
    25441 ctttataagt aaagaatact ggcaaaataa ctataatact gttaaaaacg aatatatacc
    25501 tatatcagat acatttgtat atgatgatgg agatgaaata aaaggattta taagcataat
    25561 agataaaagc tttataggag ctttatttat aaagcccaaa taccaaaatc taggtatcgg
    25621 aggtaaactt ttagattatg caactaaaaa atataaaagt ctaagcttag cagtatataa
    25681 agataataaa aaagcagttg tgttttataa taaaaaaggt tttaatatag taaaagaaca
    25741 agtaaatgaa gattcaggat ttaaagagta cataatggaa tatagtaaat aatatgatta
    25801 cattctatta aaaggataat taatttattt gccatttgat tttttaagag taatttttaa
    25861 aatagactat atgaagataa agatatttat tatagatgta agttaatcag aattgatata
    25921 gctacaaaag aggaaaataa aattgtatgg tgtatttcag atttaacaaa tttactattt
    25981 aaagataata agtttgatat aatattaaag atactttcac ctataaacta tagggtattt
    26041 gaaagaattt taaataatta tggaatgcta ataaaggttt gttaaaattt aataattatt
    26101 agattataga aaattgccat gaaattagtt catcaagaac tagtttc
//
LOCUS       DAEPIN010000057        25614 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.057, whole genome shotgun sequence.
ACCESSION   DAEPIN010000057 DAEPIN010000000
VERSION     DAEPIN010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25614
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.057"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            286..852
                     /locus_tag="KR250_002649"
     CDS             286..852
                     /locus_tag="KR250_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HBH0068571.1"
                     /translation="MRKLEEILEYNKSFVENKEYEQYVTSKHPNKKIVVLSCMDTRLT
                     ELLPKAMNLKNGDVKLIKNAGATIMHPFGSIMRSVLVAIYEFDVDEVMVVGHHGCGMC
                     NVDTDKLLGKILDRGISNDVILTLRSAGIDTKQWLHGFDSAEESVKDSIDLIKGHPLI
                     PDGIIVHGLIISPETGKLDVVVNGYEDK"
     gene            1157..1618
                     /locus_tag="KR250_002650"
     CDS             1157..1618
                     /locus_tag="KR250_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBH0068572.1"
                     /translation="MNSNSEEKLNDVFLQLMRIYFIRTHFYFDKLGLYHGQPQILFSL
                     KMKDGQSQKELADKRKVKASTTTVMIKRMEKAGLVERKQDEKDQRVSRIFLTDKGLEI
                     CNKVEEFNKELEREFFLGFSEEEKIVLRRLLMQVRDNLKAGCHKHEDEKYF"
     gene            1686..3932
                     /locus_tag="KR250_002651"
     CDS             1686..3932
                     /locus_tag="KR250_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011009877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0068573.1"
                     /translation="MFKLIKYLKKSALSIVIIVCLLVIQAVCDLSLPEYTSNIVNVGI
                     QQGGVENSVPSVIRESELNKITLFMDKSSKDKVLDNYTLLNKKDYVKYKDKYPGLKDE
                     SLYELNTKDKDTIDDLNVIFGKAILIVSGLEGDSKEVKAMKAQLMSKLPPQATQSGDV
                     DIFKLLSAMPKEQLDTLTKEMSKGFESMPESMITQSSVSYVRSEYEKIGIDTEKTQNN
                     YILFTGAKMLGIAMISMVATITVGFLAARVAASVGRNLRSGVFRKVMSFSNTEMNEFS
                     TASLITRSTNDIQQIQMLMVMLLRIVFYAPIMAIGGVIKVLNTNTSMAWIIAVAVVAI
                     LSLIVVLFSVVMPKFKLVQKLVDKLNLVSREIITGIPVIRAFSTEKHEEERFDKANKN
                     LTKTNLFVNRVMTCMMPAMMLIMNLITVLIVWRGAYSVDSGAMQVGDMMAFIQYTMQI
                     IMSFLMISMVSIMLPRASVSATRIDEVLVSDISIKDPEKSEAQEFKEDKKGLVEFKNV
                     SFMYPDAEEPILTNISFTAKPGETTAFIGSTGSGKSTLINLIPRFFDVTEGEILLGGV
                     NIKNVSQHDLREKIGYVPQKGVLFSGTIESNLKYGRKDATDEEMRTAAEIAQATEFID
                     SKPEAFKTEISQGGTNVSGGQKQRLSIARAIAKNPEVYIFDDSFSALDLKTDAALRKA
                     LKSKTAESTVLIVAQRISTILNAEQIIVLNEGEIVGIGTHKELLKNCEVYEQIALSQL
                     SKEELANE"
     gene            3925..5757
                     /locus_tag="KR250_002652"
     CDS             3925..5757
                     /locus_tag="KR250_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0068574.1"
                     /translation="MSRMGRGPMGKSMGAGQKANDFKGTMRKLIAYLSKFKISIILVI
                     VFAIGSASFSIVGPKILGKATTKIFEGLVSKVSGGNVGIDFNAIGKILTFLLFLYLIS
                     ALFSFIQGFIMSGISQKVSYNLRKEISAKLDRLPMKYFDTKTHGEILSRITNDIDTLN
                     QSLNQSMTQLITSVTTMIGVLIMMLSISGIMTLVAVLILPISMFVISRIIKKSQKYFR
                     YQQEYLGNVNGQVEETYSGQTIVKAFNREDEVIEEFDKLNDSLYNSAWKSQFLSGIMQ
                     PLMMFIGNLGYVMVSILGGWLAIKKTIEVGDIQSFIQYVRNFTQPMTQIAQVANLLQS
                     TAAASERVFEFLEEEEEVQIVENAVSIDGLEGKIDFENVNFGYNPNKTIINDFSVNVE
                     PGQKVAIVGPTGAGKTTIVKLLMRFYDVNSGSILIDGHNIKDFNRSELREMFGMVLQD
                     TWLFSGSIMENIRYGKLNATDEEVIEAAKSAHVHRFIKTLPDGYKMKLNEEASNVSQG
                     QKQLLTIARAILADPKILILDEATSSVDTRTEVLIQKAMDNLMEGRTSFVIAHRLSTI
                     RDADMILVMNEGDIVEQGNHEELLKKGGFYANLYNSQFEEDEAM"
     gene            6476..7753
                     /gene="hflX"
                     /locus_tag="KR250_002653"
     CDS             6476..7753
                     /gene="hflX"
                     /locus_tag="KR250_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HBH0068575.1"
                     /translation="MDMAKKGITVGLNINNKSEDFNELMIELENLCSACDIDVVGSIT
                     QNAKQVNRAFYIGTGKVEEILNLIKKENIEIVIFYNELSTSQLKNLEEKLNCEIIDRT
                     ALILDIFAQRAKTREAKLQVEVASLKYMLPRLIGSNENLGRQSGGVGTKNRGSGEKKL
                     ELDRRRIEEKITSLNRELDDLKFQRETQRSMRRKSNLPNVALVGYTNAGKSSIMNKLV
                     DIFKNSEEKKVFEKNMLFATLETSVRNIVLANNKEFLLSDTVGFVSNLPHDLVKAFRS
                     TLEEACEADVLLHVIDISNPSYKSHIKVTEDTLKQIGADGIPMIHVYNKIDLIDVDVL
                     DRILDSIDKEGIFVSVKKDINIDKMIKCICDSIFKDYVRCKFLIPYDKGHVVSYFNEN
                     MSIINTEYREDGAILDVECSNIEYNKYKKYALE"
     gene            7986..8483
                     /locus_tag="KR250_002654"
     CDS             7986..8483
                     /locus_tag="KR250_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBH0068576.1"
                     /translation="MDAETLMKKYMVDDDSRKQAIFASIFVMQNKLQTTCDKLDPELT
                     MKQWLLLAIADSVDETLTLTKLGELMGCSRQNVKKLAIALEKKGFIQIEQNEKDSRAI
                     CLVTKDRVQEYSKKVRGMQEKVLQLLFEDFTEKEVEQLFFGIMKLSIGIENVKNYVEN
                     NDVGE"
     gene            8533..9057
                     /locus_tag="KR250_002655"
     CDS             8533..9057
                     /locus_tag="KR250_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HBH0068577.1"
                     /translation="MKNVVVIYKSKYGSTKKYAKWLSELLSCDIFETKNINIEKVMKY
                     DTIILGGGLYACNIAGISFLKKNYKELQKKNLVVFGVGAAPYDDEVIKKLREVNFKDE
                     LNKIPCFYCRGAWDETKMSFIDYTLCSFFKKSIAKKERQGLKPAEAPLLSGEAFDWCN
                     KENLTPILDYIREL"
     gene            9369..10733
                     /locus_tag="KR250_002656"
     CDS             9369..10733
                     /locus_tag="KR250_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HBH0068578.1"
                     /translation="MDIKELVKMQRKYYNTGKTRDISFRIEQLKKLKLVVSQNEEKIL
                     LALKKDLNKSDFEGFMTEVGMFYSELNFAIKNIRKWSKIKRVKSSMVNFPSISKVVPQ
                     PYGVTLIMSPWNYPFQLALIPLVWSLVAGNCVILKPSEYSVSTSSVVKDIVEDTFSKE
                     YVAVVQGSQEESEKLLLERFDYIFFTGSTNVGKIIMKSASEHLTPITLELGGKSPCII
                     LKDANIDLTAKRLTWGKLINAGQTCVAPDYVLVHEDRKNELIEKIKYYTNKYFGDNPC
                     NNEQFPKIINQRHFNRILSLIDKDKIVYGGNYNKETLKIEPTIVDNVNWDDNIMKEEI
                     FGPIFPILTYKDLDEVIQKIIQMPNPLALYIFTKNKYLENKLLEMIPAGGCCINDTVT
                     HIATNYLPFGGIGESGMGSYHGKAGFDTFTHYKSVLKKLNLDVPIRYAPYDNKLIKVL
                     KKIM"
     gene            10872..12038
                     /locus_tag="KR250_002657"
     CDS             10872..12038
                     /locus_tag="KR250_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBH0068579.1"
                     /translation="MKINWNTKYTTIAIYTFIIAASSIIFYLVSSQIDVFSNNLDAIF
                     TTLQPFIIGFAIAYLLNFILKFYEDRIFIKSEKLKKLKQSSKRGLGLLLTYATATLIL
                     YLFMHFVLPQVIESIVGLANDIPMYVNNATKLIDKLMTDLNLDEQYFNLAVDKWNEFV
                     TYIIKFVTDLIPILGSMLKNVASSIWNIVLGLIVSVYLLIDKEKFYGLSKKITYAVFT
                     EKQAARILELTHRSNYTFGRFLGGKILDSFIIGILTFVILTLVKMPYTLLISVIIGIT
                     NIIPFFGPLFGAIPSTLIVMFVSPIKAFWLLLIILIIQQIDGNIIGPKILGDSIGISA
                     FWILFSLLVAGKLLGFIGMVIGVPMFAVIYSIIKDIVESKLDKKGLPTDTSDYM"
     gene            12155..12937
                     /locus_tag="KR250_002658"
     CDS             12155..12937
                     /locus_tag="KR250_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HBH0068580.1"
                     /translation="MAFIECKFKSKYTGGETDIIVILPVANGKELFSGKEIYDFEGKF
                     KTLYLFHGLEDDQSSWIRYTNVEHYAKNKNIALVIPRVETSFYTDMAYGHQYFTFVSE
                     ELPKFVRAIFPLSDKREDNFVAGMSMGGYGALKLALSKPKEFSAVASFSGSPDMIHEL
                     ENQQIETNADILFHDFGRPEDARNSENDLLYILKNLKERNEDIPKIYQFCGDRDFLYK
                     NNQMFKKYADSLGVDVEYEECVGGHEWRLWNLWIKKFIDIIG"
     gene            complement(13056..13709)
                     /locus_tag="KR250_002659"
     CDS             complement(13056..13709)
                     /locus_tag="KR250_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBH0068581.1"
                     /translation="MSKSEKGKETKASIISAARELFFEQGYHKTTTRQIAERANINLG
                     LISYYFSSKSEIGAIIYEDIRNEMQSLIYNYHEEGTMMMFFLAMNADLCFLLKNEAYR
                     NFYLDIAYEEAILSYQRSVTEMVMKRYVNDKESIEHDELILSCIGILAMKPEIIRVYT
                     SHKYDISSQAVIKHFTKQFLINLGHSTDLCEEILEELSKYYIDIVDNFTPIITRVRT"
     gene            complement(13733..15097)
                     /locus_tag="KR250_002660"
     CDS             complement(13733..15097)
                     /locus_tag="KR250_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HBH0068582.1"
                     /translation="MLLLIVTCAIYIWIGFYFSKKVKTASDFLIAGRNLPLPILAATI
                     AATSFGGGCLVGGVQWGAERGLWVGMYSTIGAALACFINAFFAGKLRSLSCDITPADY
                     IETRYGHSVFLRAYQSLVTPISILAIMGSQLISFGSVSTAFGIPYDIAVIIGVIVIII
                     YTYTSGMWGVAVSAFIQLAICIVFLPIVAYISLKLVGDNPLLMLQSMVKEPFFPGNKS
                     ISDFMYTVLPLVLGSIFSYESFLRYQCADNAKNAVKSSVIAGFILLFLAFPIGIIGAI
                     GGSLFPNISSVQVLPQMISTTLPPLISVLFLAAILAAIMSTADSLMTSMSAIISRDIY
                     NKLLHPNIEFNDLKNTLKYSQIASALTAIIGAIIALKFDSLLELLFWPAPLCTGVIFA
                     PFVIGLCWKGATKKGAIYAVIVSVILALLNMIGILTVFDRILVPIIGGSITIFIVSKF
                     SKKD"
     gene            15756..17186
                     /locus_tag="KR250_002661"
     CDS             15756..17186
                     /locus_tag="KR250_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBH0068583.1"
                     /translation="MSSQESKPKGNLISNFRDDMRDPKKRAIYTYSSSINLAYNFIGY
                     FVSTVTNIFLTDVAKLGVIMAGGMQIIQSFIKIAVAPITGTVFDKQPFKKGKYYPWLR
                     YAPAALALTYGIFFIVPLMKISPALLLPLMTALLLIASCAQQVVFTIIYAIYPVVAKT
                     PRDRVIGSTTTNIFKEVGKFLVGFSYPLLLVLFADVLGGEGMGYLGTYLFFAAGCLLV
                     FWFSSTEIIKSGAEEEVLQKEEKPKIKASEMAKALFTNVPLMMAFCLEFLICIRSIGL
                     GPLAPYYFKYVIEDERGLAVFLSIMPLVSVAFMFFAPVFIKICREQKLASIISFSICA
                     LCHLLVAWTPWGKTTLGVTMLLAIGGGFSNVVSIINLNFFAGSCDYGHWKSGKDLPGL
                     SMSLYPVAIQVGVLLATTIRTVLMNSMGYQADMVVTEAVKSGFINMISYSMAIPLIIA
                     VVIAILYPVSDKKLNQIREELNQRNA"
     gene            17289..18392
                     /locus_tag="KR250_002662"
     CDS             17289..18392
                     /locus_tag="KR250_002662"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HBH0068584.1"
                     /translation="MIEKLLRNTVKELHQYVPGEPIEKVKEKYGVKEIIKLASNENPL
                     GPSPKAIEAMIEMLKQGQLYPEPEANELRRKLAEKLDLKPENFIVANGADNVITLIGE
                     AFINRGDEVIYCNPTFPSYRTATIKNEGIPVEVPLTEDYKYDLQGILDKITDKTKLIC
                     VCNPNNPTGTIVDDKELEEFLKKVPENIITILDEAYIEFLTVPNYVDGLKYVRENYNV
                     IVTRTFSKIYGLAGLRVGYGIAKDEIIRTLFTVKEPFSANRVAISGATAALDDEEFIK
                     ETYELNRVGMAYFKEEFTKMGFDVVDSQSNFLYVDMKTDIPKLFEDLKKRGFVIRPSA
                     THARVSIGTMEENKKFVAVLKEILNIKGEPSIG"
     gene            18407..18643
                     /locus_tag="KR250_002663"
     CDS             18407..18643
                     /locus_tag="KR250_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBH0068585.1"
                     /translation="MKRGVDMNLKINAERCKSCEYCVISCKKGALKISSKINKEGYAH
                     VEVDESKCILCGICYQVCPDNVFEIIKEEACQEA"
     gene            18660..19709
                     /gene="vorB"
                     /locus_tag="KR250_002664"
     CDS             18660..19709
                     /gene="vorB"
                     /locus_tag="KR250_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HBH0068586.1"
                     /translation="MKKMWKGNHAIAEAALRGGCEFYAGYPITPQTEVIEFLSHRMSE
                     LGRTFIQSENEMAAIYMVYGAYASGMRSMTSSSGPGISLKQEGISYLCANHYPCVIVN
                     VQRWGPGLGSLDSAQTDYLRDTRGGGNGDYHLIVYAPNSIQETVDLMYNSFDVAEKYR
                     VPVEILTEAALGQMMEPVEFPEFKKREGDLGWTYDGSNRDHAKVADNQKPTFCMEKRM
                     RITENEQQWEDYQVEDAEYVFVAFGIPSRTTMNAVRRLREQGEKVGIIRPITVWPFPY
                     KAFEKVSKDVKGFISVESTDTGQLVEDVALTSKKVCKENVPVYGLFSGNHIPKTLQVM
                     DTYSKIKSGEIKEVF"
     gene            19711..20451
                     /locus_tag="KR250_002665"
     CDS             19711..20451
                     /locus_tag="KR250_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HBH0068587.1"
                     /translation="MATEKLTPSVVSIPNKFCPGCGHGIVNRIIAEVIEEHGYEKNHV
                     LTLGVGCVCNMNFSWNGDKMQTAHGRASSTAIGVKVALPDTLVMTYQGDGDAYVIGLS
                     ETLNTAYRNHNVTVFVINNNNFAMTGGQMSWTTMPGQVTTTSVNGRDIKTTGSPLKVP
                     EMVANFFDVAYVARGSVHSPKEIINLKKYIKNAIEAQLNGEGYSLVEILAPCPTNWGV
                     SLEKSIKWMEEEIVPYYALGEFKQRDGE"
     gene            20454..20993
                     /locus_tag="KR250_002666"
     CDS             20454..20993
                     /locus_tag="KR250_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HBH0068588.1"
                     /translation="MEKELVFAGFGGQGVLTLGLIVAESALELGKQVTWMPAYGPTMR
                     GGKAYSVVKFSDESIGGPDMEEIDVLVAMNKPSLDYINLVKEGGTVVINTSAIDENVT
                     LREDISVVKINCQELAQKVNNPKAANIVVLGALIAKTGLFEKELALKTMCDFFEEKGK
                     GKFNVQNEAAFMEGYNNAI"
     gene            21117..23216
                     /locus_tag="KR250_002667"
     CDS             21117..23216
                     /locus_tag="KR250_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HBH0068589.1"
                     /translation="MSLEKLIRPKSIAIVGVTDKLGFGRSAALSIVKSKETDRVYYVN
                     PKREELFGRKCYKTIQEVPEVVDCVVVCTPRNVVPSVLKDSGELGTKAAVVYASGFAE
                     EGTEEGTDLENQLIEISNTYDMKILGPNCMGLLNCIDKVNLWAGGSKWDLDTKKPGIG
                     IVGQSGFITAEIVSSDYFNISYGFSTGNGNIVTLEDAVDFLVDDDYASVIAIYLEGLK
                     NPQKFIDALKRAAQKRKPVIILKSGRSEKGAISAASHTGNLAGSSKAFESIFEKYGVI
                     SVENLEQFMCLAQAFSVLDGNLPANSNFAAINFSGGENTICADLAEENGVELAEISSE
                     TKEEMKKYLPGFATPKNPLDATTALFHEKDMIVGLLHTFEKDDSVGFTMVGANIRDEE
                     NEMHETLCKAVSEAREQGLKKPVFAVPTLEGNRYLDYRTRLEDNQVPIMSSVGTTFTC
                     FNKMAKFIDYDYSKRTLEFKAVKKRESNNVVALSELDSKIEMKKYGVPVPGQGNAKSI
                     DELDEILKYIKYPVVLKINSSEILHKSDVGGVKIGIENRDEAVDAYNEILTNVKKAKP
                     DANIDGILVQEMVESGIEIIIGITNDDQFGPMLLVGLGGVFVEVFKDTTLYPLPINHD
                     EAIMMLKKLKSFKLLNGYRGSEPCDIDALADMMVKLGKYAYENKDEVKEIDLNPVFVY
                     PKGKGVCAVDALIVKYK"
     gene            complement(23483..23998)
                     /locus_tag="KR250_002668"
     CDS             complement(23483..23998)
                     /locus_tag="KR250_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HBH0068590.1"
                     /translation="MISAKMEKLLNEQINHELYSAYLYLSMSSYLEAEGLKGFSNWFY
                     VQYKEETDHAMFFYKYLHNVGGKVQLDAIPMPDSDFTSAMDILERTLAHEKKVTALIN
                     NLAAVANSESDFRTSQFLLWFISEQAEEETNCEDNIKRVKLAGEGGLFFVDQEFANRT
                     YAAPTNPPVTI"
     gene            complement(24090..25301)
                     /locus_tag="KR250_002669"
     CDS             complement(24090..25301)
                     /locus_tag="KR250_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HBH0068591.1"
                     /translation="MSNKQGSNIKDVKIKNKLIILKLLSTNTPMSRVDLAKSTGLTKM
                     TLSNLVTELINENMIREIESSTLNAHSSGRKPILLDISNISPCICGILIRRNICKVII
                     SDLKGNIVKQLSETYPKCLSNDVLKDIIKRMFTKIMKINKRDIIGIGISSIGPVDDIN
                     GTILNPPDFGNVSNLNIVDFIKEMSDLPIFLINDANSGALAEKMYGLGKNISNYIYLH
                     IMNGIGAGLVLENKLHTGNLGQSGEIGHTSINFSGPLCSCGNNGCLDYYTSVSNLIKR
                     IESLSHIYPNSPLINCKDFSLVKLIDEANNKDSLAMFLLDEFCTYVSYALVNTLTLID
                     CSSIIIGYDFNIPGTFIENTLLKKLTASASFSKYKKISVRHSNFGANAPLIGSIAIVS
                     YNFFNGSINFY"
BASE COUNT         9881 a         2921 c         4442 g         8370 t
ORIGIN      
        1 tctaattata tataggtgaa ataaaaaatt aattataagt aaggcattta gaataaaatt
       61 ttaaaaaaat tataattaaa aaaataatga aaatatataa tattagttta gattataaaa
      121 aataaatata caaatactta taaatacaag ttatatacaa gatttatagt agagtaataa
      181 tcttataaaa taatttaata aatataaatt aaattatatg tgtatatatt gtactttaat
      241 agtgatataa taataaaaaa atttttaaag agggtgatat tacatatgag aaaacttgaa
      301 gagatattag aatataataa atcatttgta gagaacaaag aatatgaaca atatgtgaca
      361 tcaaaacatc caaataaaaa aatagtagtc ttatcttgta tggatacaag attaactgag
      421 cttttgccta aggctatgaa tttgaaaaat ggagatgtga aactaataaa aaatgcagga
      481 gctactataa tgcatccttt tggaagtatt atgagaagtg ttttagttgc aatttacgaa
      541 tttgatgtag atgaggttat ggttgtaggg catcatggat gtggtatgtg taatgttgat
      601 acggataagt tacttggtaa aattttagat agaggaatat ctaatgatgt aatacttaca
      661 cttcgtagtg ctggaattga tacaaaacaa tggttacatg gattcgattc tgcagaagaa
      721 tctgtaaaag atagtattga tttaataaaa gggcatcctt taattccaga tggtattatt
      781 gtacatggtc taattatatc acctgaaact gggaagttag atgttgttgt aaatggatat
      841 gaagataagt agagtatcat aatgttatat aatcaagata ctgttgaata agatgttgtt
      901 tcaaaattta ttttgggaca acttttttat ttgaaaatat atgaaaaggt ttattttaag
      961 ctttacaata aaaaatataa gtagctgact aattaaaaat cttactatta tttattaaaa
     1021 ttataactat atgatataaa aatatttaat aaatgaaatt tatatgaatt ttttataaat
     1081 atattgaaaa aatgcatgca gtatagtaaa ataatgctta gccgactaag taatataaaa
     1141 aataaggagg gcaagtatga atagcaattc tgaagaaaaa ttaaatgatg ttttcttgca
     1201 attaatgcga atatatttta taagaactca tttttatttt gataaattag gcttatatca
     1261 tggtcaaccc caaatacttt tttctttaaa gatgaaagat gggcaaagtc aaaaagaatt
     1321 agcagataaa aggaaagtaa aagcatctac aactacagtt atgataaaga ggatggaaaa
     1381 agctggactt gtggaaagaa aacaagatga aaaggaccaa agagtatcta ggattttttt
     1441 gacagacaaa ggtttagaaa tatgtaataa agttgaagaa tttaacaagg aattggaaag
     1501 agagtttttt ttaggattta gtgaagaaga aaaaatagta cttagaagac ttctaatgca
     1561 agtacgagat aatttaaaag caggatgtca taagcatgaa gatgaaaaat atttttaaag
     1621 tttaaaataa tgtttttcaa tttaatgtta ttagactaat taatattaca gaagggaaga
     1681 aatgtatgtt taaattaatt aaatatttaa agaaatcggc attatcaata gtaataatag
     1741 tctgtctatt ggtaattcaa gcggtatgtg acctttcttt accagaatac acatccaata
     1801 tagtaaatgt aggtatacaa caaggtggag tagaaaactc tgttccatct gtgattagag
     1861 agagtgaatt aaataaaatt actttattta tggataaaag tagtaaggat aaagtactag
     1921 ataattatac tttgctgaat aaaaaggatt atgttaagta taaagataag tacccaggtc
     1981 taaaagatga atctctttat gagttaaata ctaaagacaa agacactata gatgatttaa
     2041 atgttatttt tggtaaagct attttaatag tatcagggct tgagggcgat agtaaagagg
     2101 taaaagctat gaaagctcag ttaatgagta aacttccacc acaagctact caaagtggag
     2161 atgttgatat atttaaattg ttatcagcaa tgccaaagga acagttagat acattgacta
     2221 aagagatgag caaaggcttt gaaagtatgc cagagagtat gataacacaa agctctgttt
     2281 catatgtaag gtcagaatat gagaaaatag gtattgatac agaaaaaact caaaataatt
     2341 atatattatt tactggtgct aaaatgcttg gtatagcaat gattagtatg gtagctacaa
     2401 taacagtagg atttttagca gcaagagtag ccgcaagtgt aggtagaaat ttaagaagtg
     2461 gagtatttag aaaagttatg tcattttcaa atactgaaat gaatgaattt tctacagctt
     2521 cattaattac tcgtagtaca aatgatattc aacagataca aatgttgatg gttatgttgc
     2581 ttagaatagt attctatgca cctataatgg ctataggtgg agtgataaag gttttaaaca
     2641 caaatacttc aatggcctgg attatagcag tagcagtagt tgctatctta tcattaattg
     2701 tagtgttatt ctcagtggtt atgcctaagt ttaaactggt tcaaaagctt gttgataagt
     2761 taaacttagt aagccgtgaa ataattactg gtattccagt tatcagagct tttagtactg
     2821 aaaaacatga agaagaaaga tttgataaag caaataaaaa tttaactaaa acaaatcttt
     2881 ttgtaaatag agttatgaca tgtatgatgc ctgctatgat gcttataatg aaccttatca
     2941 cagttcttat agtttggaga ggtgcataca gtgttgattc tggagcaatg caagttggag
     3001 atatgatggc atttatacaa tatacaatgc aaataataat gtcattctta atgatatcaa
     3061 tggtatctat tatgcttcca agagcatccg tttcagctac acgtatagat gaagtattag
     3121 tgtcagatat aagcataaaa gacccagaaa aatcagaggc acaagagttt aaggaagata
     3181 aaaagggtct tgttgaattt aaaaatgttt cgttcatgta tccagacgca gaagaaccta
     3241 tattaacaaa tatatctttt acagcaaagc caggagaaac aactgccttt ataggaagta
     3301 ctggtagtgg taagtcaact cttataaatt taattcctcg tttctttgat gttactgaag
     3361 gagaaatatt acttggtgga gttaatataa aaaatgtttc tcaacatgat ttaagggaaa
     3421 aaattggata tgtacctcaa aagggagtat tgttttctgg tactatagaa tccaacttaa
     3481 aatatggtag aaaagacgca acagatgaag aaatgagaac agcagctgaa atagctcaag
     3541 ctacagaatt tatagattct aaaccggaag cttttaaaac tgaaatttct caaggtggaa
     3601 caaatgtatc tggaggtcaa aaacaaagac tttctattgc aagagcaata gcaaaaaatc
     3661 cagaagttta tatatttgat gatagttttt cagcacttga cttaaaaact gatgcagctc
     3721 ttcgtaaggc tttaaaatca aaaactgctg aaagtacagt tctaatagtt gcacaaagaa
     3781 ttagtactat cttaaatgca gaacagataa ttgttttaaa tgagggcgaa atagttggaa
     3841 taggaacaca taaagagctt ctaaaaaatt gcgaagttta tgagcaaata gctttatcac
     3901 aactttcaaa ggaggagcta gctaatgagt agaatgggaa gaggtcctat gggaaaatct
     3961 atgggagcag gacaaaaagc aaatgacttt aaagggacta tgcgaaaact aattgcatat
     4021 ttatctaaat ttaaaatatc aataattcta gtaattgtat ttgctattgg tagtgcgtcg
     4081 ttttctatag taggtccaaa aattttagga aaggcaacta caaagatatt tgaagggcta
     4141 gtgagtaaag tttctggagg aaacgtaggt atagatttta atgcaattgg aaaaatctta
     4201 acctttttac ttttccttta tttgataagt gcactgttct cttttataca aggatttata
     4261 atgagtggaa tttctcaaaa agtatcctat aatttaagaa aagagatttc agcaaaattg
     4321 gatagattgc caatgaagta ttttgatact aagacgcatg gtgaaatatt atcgagaatt
     4381 acgaatgata tagatacttt aaatcaaagt ttaaatcaaa gtatgacaca gcttataaca
     4441 tcagtaacaa ctatgatagg tgttttgata atgatgttat ctataagtgg aattatgaca
     4501 ttagtggcag ttttaatatt accaatatca atgtttgtaa tttcaagaat aataaagaaa
     4561 tcacaaaaat actttagata tcaacaagaa tatttaggaa atgtaaatgg tcaagttgag
     4621 gaaacttaca gtggccaaac aatagtaaaa gcatttaatc gtgaggatga agtaattgaa
     4681 gagtttgata aactaaatga tagcctgtat aattcagcgt ggaaatctca atttttatca
     4741 ggtataatgc aaccacttat gatgtttatt ggaaacttgg gatatgtaat ggtatcaatc
     4801 ttaggaggtt ggcttgcaat taagaaaaca attgaggttg gtgacataca gtcatttata
     4861 caatatgtaa gaaactttac acagccaatg actcagattg cacaggttgc caatttattg
     4921 caatcaacag cagcagcttc tgaaagagta tttgagtttt tagaagagga agaagaagtc
     4981 caaattgttg aaaatgcagt ttcaatagat ggtttagaag gtaaaattga ttttgaaaat
     5041 gtcaactttg gatataaccc aaacaagact ataataaatg atttctcagt aaatgttgaa
     5101 cctggtcaaa aagttgctat tgttggacct acaggagcag gtaaaactac aatagttaaa
     5161 ctgttaatga gattttatga tgttaatagc ggttcaatac tgattgatgg tcataatatt
     5221 aaagatttta atcgtagtga gcttagagaa atgtttggta tggttcttca agatacatgg
     5281 ttatttagtg gttcaattat ggaaaatata agatatggta agttgaatgc tacagatgaa
     5341 gaagttatag aggctgctaa atctgcacat gttcatagat ttattaagac attaccagat
     5401 ggatataaga tgaaattaaa tgaagaagca agtaatgttt ctcaaggtca aaaacaatta
     5461 ttgactatag ctcgtgctat tttagctgat cctaaaattt taatattaga tgaagctaca
     5521 agttctgtcg atactagaac agaagtattg attcaaaaag ctatggataa tcttatggaa
     5581 ggtagaacaa gttttgttat agcccacaga ttatcaacta tacgtgatgc tgatatgatt
     5641 cttgtaatga atgaaggaga tatagttgaa caaggaaatc atgaggaact tctaaagaaa
     5701 ggcggattct atgccaatct ttacaattct caatttgaag aagatgaagc aatgtaacaa
     5761 aaaactatct tagcatagac atgtaattaa taagataaaa taaatgaggg tgccttaaaa
     5821 tgaacttttt aattcataag gcactctttt gtgtatgaaa taaagtgtat ttttatcatt
     5881 ttaacaatga aaaagaggat tctctatatt ttgagatagc cacataattt tatgtcttta
     5941 caaagtgttt attttattat aatatgtagt aaggaaaatg ttggaggtga ttatcaaaat
     6001 gtatatttaa ttttattctt taaattttat tataaataga atttaatttt aatagttgat
     6061 gattatattg aagtatatgt ttttattact tttattaatc atgcaaataa ctacttaaat
     6121 ttaagaatag ggttaaatat aaaatgataa ttacatataa gttaattaaa ctaatgtatt
     6181 tttaatatat atttagaatt ttaatccttc ttatgatata agtagcttgt tttgtattac
     6241 ttagatttgt aaggagtttt tttattgaaa aaactgcata atatatttgg gaggaggtgt
     6301 aagaaagtga ttttttatat aagattgaga aaataatgca cgattatggt tattataata
     6361 gtaaataaga agtgtcattt aaggataaga taaaaagcaa aattcttgat gaatttaatt
     6421 atagagccat aatctcgtgc tatagattaa ataaaaaata gcagataagg agaatatgga
     6481 tatggcgaaa aaaggaataa cagtaggatt aaacataaat aataaatctg aggactttaa
     6541 tgaactaatg attgaacttg aaaacttatg ttcagcatgt gatattgatg ttgtagggag
     6601 tataactcaa aatgcaaagc aggttaatag agcattttac attgggacag gtaaagttga
     6661 ggaaatatta aacttaataa agaaagaaaa tatagagata gttatatttt acaatgaact
     6721 ttctacatca caactaaaaa atcttgaaga aaagcttaat tgtgaaatta tagatagaac
     6781 ggcattgata cttgatatat ttgcacaaag ggcaaaaact agagaagcta aacttcaagt
     6841 agaagtagca agtttaaaat atatgttacc aagacttata ggttcaaatg aaaatttagg
     6901 acgtcaaagt ggtggagttg gtactaaaaa tagaggttct ggtgagaaaa aattagaact
     6961 tgaccgtaga agaatagaag aaaagataac atctttaaat agggagttgg atgatttaaa
     7021 attccaaaga gaaactcaaa gaagtatgag aagaaaatca aatcttccaa atgtagctct
     7081 agtcgggtat acaaatgctg gtaaatcttc aataatgaat aaactagtag atatatttaa
     7141 aaactctgaa gaaaagaaag tgtttgagaa aaacatgtta tttgctacac ttgaaacttc
     7201 tgtcagaaat atagttcttg caaataacaa agaattttta ttatctgata ctgttggttt
     7261 tgtaagtaat ctaccacatg acttagtaaa agcatttagg tcaacattag aagaagcatg
     7321 tgaggcagac gtactgttac atgttattga tatttcaaat ccaagctata aaagtcatat
     7381 taaagtgaca gaagatactt taaaacaaat aggtgcagat ggtataccta tgatacatgt
     7441 gtataataaa attgatttaa tagatgtaga cgtattggat agaattttag atagtattga
     7501 taaagaaggt atatttgttt cagttaaaaa agatataaat atagataaaa tgataaaatg
     7561 tatttgtgat agcatattta aagattatgt aagatgtaag ttcttaattc catatgataa
     7621 aggtcatgta gtttcttatt ttaacgaaaa tatgagcatt ataaatacag aatacagaga
     7681 agatggagct attttagatg tagaatgtag caatatagaa tataataagt ataaaaagta
     7741 tgctttagag taaattttga aaaacttatt atagcctaaa atatatttaa tttataattt
     7801 attataaatt gattgtaaat atgattttat gaaacggtat agccataatg ctatgccgtt
     7861 ttgccattta taataaaaat aaatttattg acataatagt aactaattac tataatataa
     7921 agtaattagt tactatttaa aattatcaaa aaattaaaat ctaagataaa gagaagtgta
     7981 taataatgga tgcagaaact ttaatgaaaa aatatatggt agatgatgat agtagaaaac
     8041 aagctatatt tgcaagtata tttgtaatgc aaaataaact tcaaactaca tgtgataaat
     8101 tagacccaga attaacaatg aaacaatggt tacttttagc tattgcggac tctgtagatg
     8161 aaactcttac attaaccaaa cttggagaat taatggggtg ctcaagacaa aatgtaaaaa
     8221 aactagctat tgctttagaa aagaaaggtt ttatacaaat tgagcagaat gaaaaagatt
     8281 caagagctat ctgtcttgtg acaaaagata gagtgcaaga gtattctaaa aaagtaagag
     8341 gtatgcagga aaaagtatta cagcttttat ttgaagattt tacagagaaa gaagtggaac
     8401 agttattttt tggaattatg aaattaagta taggaattga aaatgtgaaa aattatgtag
     8461 aaaataatga cgttggagaa taagcaagta tcaaatcaac tttgtatgac atcagagagg
     8521 gggataattt atatgaaaaa tgtagtagtg atttataaga gtaagtatgg ttcaacaaaa
     8581 aaatatgcta aatggctttc agaattattg tcttgtgata tttttgaaac taagaatatt
     8641 aatatagaaa aggttatgaa gtatgatacg attattttag gaggaggtct ttatgcttgt
     8701 aatatagcgg gaatttcttt cttaaaaaaa aattataaag aattgcagaa aaaaaattta
     8761 gtagtatttg gcgtaggagc tgctccatat gatgatgaag taataaagaa attaagagaa
     8821 gtaaatttta aggatgaatt aaacaaaatt ccttgctttt attgtcgtgg ggcatgggat
     8881 gagacaaaaa tgtcttttat agattatact ctttgtagtt tttttaaaaa atctatagct
     8941 aaaaaagaac gtcaaggtct taaaccagca gaagcacctt tattgtctgg agaagcattt
     9001 gattggtgta ataaagaaaa tttaactcca atacttgatt atataagaga actttaagag
     9061 gtatatattt ataattattt attatctaaa gttagatttc atagaaactt actagacatt
     9121 atttataaaa taatattatt aaagatggat aatttaattg attcaataag gttataattt
     9181 taaaaaatgt ttattttaaa ataaattatt ttataagctt acttatactt aataaggggc
     9241 tttttttatt ggaagattta tgttatattg agatgaattt attatgatat aaaaaataat
     9301 gttaggataa atttaaatct ttttctaaaa atgtattaat ataattctta aagaaaggat
     9361 ttttaataat ggatataaaa gagttagtta aaatgcaaag aaaatattat aatacaggta
     9421 aaacaagaga tatatcgttt cgtatagaac agttgaagaa acttaagtta gttgtatcac
     9481 aaaatgaaga aaaaatactt ttagcactaa agaaagattt aaataaatcg gattttgaag
     9541 gttttatgac agaagtaggc atgttttata gtgaattaaa ctttgctata aagaatataa
     9601 gaaaatggag caaaataaaa agagttaaat caagtatggt taattttcca tctatatcca
     9661 aagttgttcc acaaccttat ggtgttactc tcataatgtc accttggaat tatccatttc
     9721 aattagcact tataccatta gtttggtcat tagtagcagg gaattgtgtt atattaaaac
     9781 cttctgaata ttctgtgtca acatctagtg tagttaaaga catagtggaa gatacttttt
     9841 caaaagaata tgttgcagtt gttcaaggaa gtcaagaaga aagtgaaaag ttactattgg
     9901 aaagatttga ttatatattt tttacaggaa gtactaatgt tggtaaaata atcatgaaat
     9961 cagcatcaga gcatttaact cctattactt tagaacttgg aggaaaaagt ccttgtatca
    10021 ttttaaaaga tgctaatata gaccttactg ccaaaagact tacttgggga aaactaataa
    10081 atgcaggaca aacctgcgtg gctccagatt atgttttagt gcatgaagat agaaaaaatg
    10141 agttaataga gaaaataaaa tattatacta ataagtattt tggagataat ccatgtaata
    10201 atgagcaatt tcctaaaatc ataaatcaga gacattttaa tcggatatta tctcttattg
    10261 ataaagataa gatagtctat ggtggaaatt ataataagga gacattaaag atagagccaa
    10321 ctatagtaga caatgttaat tgggatgata atataatgaa ggaagaaata tttgggccta
    10381 tatttcccat tttaacatat aaagatttag atgaagtaat acaaaaaatt atacaaatgc
    10441 caaatccatt ggcactttat atttttacaa aaaataagta cctagaaaat aaacttttag
    10501 aaatgattcc agcaggcgga tgttgtatca atgatacagt tactcatata gctacgaatt
    10561 acttaccttt tggtggtatt ggtgaaagtg gtatgggctc atatcatgga aaagctggat
    10621 tcgatacatt tactcactat aagagtgtgt taaaaaaatt aaatttggat gtccctatta
    10681 gatatgctcc atacgataat aaattaatta aggttcttaa aaagataatg taggttatat
    10741 tgtagaataa taaatataaa taaaattgag tatttgtatt ctgaatatat tttattttta
    10801 atttggtata tttgttataa aagtggtgat tttgtgataa aatttaatta tatagaaata
    10861 aggaggaaaa aatgaaaatt aattggaata caaaatacac aacaatagca atttatacat
    10921 ttataatagc tgctagtagt attatatttt atctagtatc atcacaaatt gatgtatttt
    10981 ctaataattt agatgctata tttacaacat tacaaccttt tataatagga tttgctattg
    11041 catatcttct taattttatt ttaaagtttt atgaagacag aatttttata aaaagtgaga
    11101 aattaaaaaa actaaaacaa agttcaaaaa gaggcttggg tcttttatta acatatgcaa
    11161 cagctacatt gatactttac ttatttatgc atttcgtatt gccacaagta attgagagta
    11221 ttgttggact agcaaatgat atacctatgt atgtaaacaa tgcaactaag cttattgata
    11281 aattaatgac tgatttaaat cttgatgagc aatactttaa tttggcagtt gataagtgga
    11341 atgagtttgt tacatatatt ataaagtttg taacagattt aatcccaata ttaggaagta
    11401 tgttaaagaa tgtagcctct agtatatgga atattgtact aggattaatt gtttcagtat
    11461 acttattaat tgataaagaa aaattctatg gattaagcaa aaaaataact tatgcagtat
    11521 ttacagaaaa acaagctgcc agaattttag aattaactca tagaagtaac tatacatttg
    11581 gaagattttt aggtggcaaa atattagatt cattcattat aggtattcta acatttgtaa
    11641 tacttacgtt agttaaaatg ccatatacat tattaatatc agttataatt ggtattacaa
    11701 atattatccc tttctttgga cctttatttg gagctatacc atctacgcta atagtgatgt
    11761 ttgtatctcc tataaaagct ttctggttat tgttgattat attaataata caacagatag
    11821 atggaaatat tataggacct aaaatacttg gagattcaat tggtatatct gcattttgga
    11881 ttttgttctc cttattggtt gcaggaaaat tattaggttt tattggaatg gttataggag
    11941 ttccaatgtt tgcagtaatt tattctataa ttaaggatat tgttgaaagt aaattggata
    12001 aaaagggatt accgacagat acttccgatt atatgtagct aaaaaatatt atgtataatt
    12061 aaaaatatat aaattaagca atatttaaag cactgagaaa gttaaactct tggtgctttt
    12121 tagatataaa attattatag aagaggtgtt ttttgtggca tttatagaat gtaaatttaa
    12181 atctaaatat actggaggag aaactgatat aattgtaatt ttgcctgtag caaatggaaa
    12241 agaacttttt agtggtaaag agatatatga ttttgaaggt aaatttaaaa cattatactt
    12301 atttcatggt ttagaagatg accaaagctc atggattaga tatactaatg tagagcatta
    12361 tgctaaaaat aaaaatatag ccctagtaat accaagagta gaaactagtt tttataccga
    12421 tatggcgtat ggacaccaat attttacttt tgtaagcgag gaattaccta aatttgttag
    12481 agctatattt cctttatcag ataaaaggga agataacttt gtagctggta tgtctatggg
    12541 gggatatgga gctttaaaat tagcgctttc aaaaccaaaa gagttttcgg cagttgcaag
    12601 tttttctggt tcgcctgata tgattcatga gttagaaaat caacaaatag aaaccaatgc
    12661 agacattcta tttcacgatt ttggtagacc agaagacgct agaaatagtg agaatgattt
    12721 actttacata cttaagaacc ttaaagaaag aaatgaagat attcctaaaa tttatcaatt
    12781 ttgtggtgat agagattttt tatataaaaa taatcagatg tttaaaaaat atgcagattc
    12841 tctgggtgta gatgttgaat atgaagagtg tgtagggggg catgaatggc gtctttggaa
    12901 tttatggata aagaaattta tagatataat tggatagtac acttatggta ttaacaggta
    12961 tactataaaa tatctttaaa tatgataaag actgaaatcc taaaatcaaa ttatgatttt
    13021 aaaatttcag tctctaagaa tttaataaaa ttttattaag ttcttacacg agttattatt
    13081 ggagtaaaat tgtcaactat atctatatag tatttactca attcctctaa aatctcttcg
    13141 cataaatccg tactatgacc aagattaata agaaactgtt ttgtaaaatg ctttattaca
    13201 gcttgagaag atatatcgta cttgtgtgaa gtataaactc taattatttc aggtttcata
    13261 gcaagtatac caatacaaga aagtattaat tcatcatgtt ctatagattc tttatcatta
    13321 acatatcttt tcatgaccat ttcggttaca cttctttgat agcttaaaat agcttcttca
    13381 taagctatat ccaaataaaa atttctatat gcttcgtttt ttagaaggaa acaaaggtca
    13441 gcattcatag caagaaagaa catcatcata gtaccttctt catggtagtt atagattaaa
    13501 gactgcattt catttcttat atcttcatat attatagctc ctatttcgct tttacttgag
    13561 aagtaatagc ttattagacc taaatttata ttagctcttt ctgcaatttg cctagttgtt
    13621 gtcttatgat aaccttgctc aaaaaataat tctctagcag cacttataat actagctttt
    13681 gtttctttgc ccttttctga ttttgacata tttactgtgt atacctctct tttcaatctt
    13741 tttttgaaaa tttgctaact ataaatattg taatagaacc acctattata ggaaccaaaa
    13801 ttctgtcaaa tacagtaaga ataccaatca tatttaataa tgccaatata acacttacta
    13861 taacagcata gatagctcct ttttttgtag cacctttcca gcaaagacca attacaaatg
    13921 gtgcaaatat tacacctgta cataatggag ctggccaaaa caataattct aataaactat
    13981 caaatttaag tgctatgatt gcacctatta tagctgtcaa agctgatgca atttgagaat
    14041 actttagagt attttttaaa tcattaaatt ctatattagg atgtaataat ttgttataaa
    14101 tatctcttga tataatagca ctcattgagg tcattaatga atcggcagta gacattatgg
    14161 cagccaatat agcagctaag aataatacag atattaaagg tggcaaagta gtcgatatca
    14221 tttgaggtaa aacctgaaca gatgatatat ttggaaataa actaccacca atagctccaa
    14281 ttattcctat agggaaagca agaaataata gtataaatcc tgcaattaca ctacttttaa
    14341 ctgcattttt agcattatca gcacattgat atcttaaaaa actttcataa gaaaatattg
    14401 agcctaaaac taatggtaaa actgtataca taaaatcact aatcgattta tttcctggaa
    14461 agaatggttc ttttaccatt gattgtaaca taagtaaagg gttgtcgcca actaatttta
    14521 agcttatata agctactatt ggcaaaaaca ctatacaaat agcaagttga ataaatgctg
    14581 atactgcaac tccccacatt cctgatgtat aagtatatat aatgataact ataactccta
    14641 taattacagc tatatcataa ggaataccaa atgcagtaga tacagaacca aaagaaatca
    14701 attgactacc cataatagct agtatagata ttggtgttac aagactttga taagctctta
    14761 aaaatacact atgaccatat ctagtttcaa tataatcagc aggtgttata tcacaagata
    14821 agcttcttaa ttttcctgcg aaaaatgcat ttataaagca agctaaagct gcacctatag
    14881 tagaatacat tccaacccaa agtcctcttt cagctcccca ttgaactcca ccaactaagc
    14941 accctcctcc aaaagatgta gcagcaatgg ttgcagctag tataggcaaa ggcagatttc
    15001 tacctgcgat taggaaatcg gatgctgttt taactttttt tgaaaaataa aaaccaatcc
    15061 atatgtatat tgcacacgta acaattaaaa gtaacatagt cttttctcct ttataaaata
    15121 ttttaataaa atatacaaaa tatatttaaa atatatttta tataaaagta gtacgatatt
    15181 tgttttattg gttttagatt aatatagcta taataattta tgatattaga tggaaattac
    15241 gtaaaaatag aaaattgaaa attctgaaat ttttgtatat tgatatttat ctcatagatt
    15301 cttttttgta attgtaacac gcttttattt ttaaatcaat agtacatgta taaaaataaa
    15361 aggaatttaa ataagaaaaa gatattttta atatctggat gtagtataga aatatcttga
    15421 ataaattatt cttgaataaa attataaaaa attaaaatca ctagaaatat gataaaataa
    15481 tttaaaatac gcatttcaat gacatttcaa aacaataaaa taataaaaaa caaacctaaa
    15541 ttaagtctaa atagcttttt agttaatttt agtagtaaaa aataaaactt aaaaagtgtt
    15601 tgacaattat gaattttgag aatataatta aattaaaatt atacacatat aaaaatatac
    15661 tcctgaatca agaatgagat atttaagatt caaaatatat attattatac gtgaataata
    15721 aaaaagtaag aaaattttat aagggagggg tagatgtgag tagtcaagaa agcaaaccaa
    15781 aaggaaatct tatatcaaat tttagagatg atatgagaga ccctaagaaa agagcaatat
    15841 acacatattc aagttctata aacttagcgt acaattttat aggttacttt gtatcaactg
    15901 ttacaaatat ttttttaact gatgtggcaa aacttggtgt aattatggca ggtggtatgc
    15961 aaattataca atctttcata aaaattgctg tagctcctat aacaggtaca gtatttgaca
    16021 aacaaccatt caaaaaagga aagtattatc catggcttag atatgcacca gcagcgttag
    16081 cattaacata tggaatattt ttcatagttc cattaatgaa aataagtcca gcattacttt
    16141 tacctttgat gacagcttta ttattaatag catcttgtgc tcagcaagta gttttcacta
    16201 taatttatgc tatttaccct gtagttgcta agacacctag agaccgagtt ataggctcaa
    16261 ctactacgaa tatatttaaa gaagttggta aattcttagt aggtttttca tatccgttat
    16321 tattagtttt atttgcagat gtattaggtg gagaaggaat gggttattta ggtacctact
    16381 tattctttgc agcaggatgc cttttagtat tttggttttc ttcaacagag ataataaaat
    16441 ctggagcaga agaagaagtt cttcaaaaag aagagaagcc aaagattaaa gcaagtgaaa
    16501 tggcaaaagc tttattcact aatgtaccac ttatgatggc attttgttta gaatttctta
    16561 tatgtataag atctatagga ttaggacctt tggctccata ttattttaaa tatgtaatag
    16621 aagatgaacg tggattagca gtatttttat caatcatgcc tttggtaagt gtagcattca
    16681 tgtttttcgc accagtgttt ataaaaatct gtagagagca aaaactagct tcaataattt
    16741 ctttttctat atgtgcacta tgccacttat tggttgcatg gactccttgg gggaaaacta
    16801 ctttaggagt aactatgttg ctggcaattg gtggaggttt ttcaaatgta gtatcaataa
    16861 taaatcttaa tttctttgcc ggttcttgtg attatgggca ttggaaatca ggaaaagact
    16921 tacctggctt aagtatgtca ctatatccag ttgcaataca agtaggagta ttacttgcaa
    16981 ctactataag aacagtttta atgaactcaa tgggatatca agctgatatg gtagtcacag
    17041 aagcagttaa atcaggtttt ataaatatga tttcttattc tatggcaata cctttaataa
    17101 tagctgttgt tatagcaata ctatatccag taagtgacaa gaaattaaac caaataagag
    17161 aagagttaaa tcaaagaaat gcttagttaa aagaaagtta agtagatggt taatttaagc
    17221 tattaaagta agtagacgca atagcgttta tataaatgaa aatacatata cagagggggc
    17281 ttttaaaaat gatagaaaaa ttattgagaa ataccgtaaa agaattacat caatatgttc
    17341 caggtgaacc aatagaaaaa gttaaggaaa aatatggtgt aaaagaaata ataaaattag
    17401 cttcaaatga aaatcctcta ggaccatcac caaaagcaat agaagctatg attgaaatgt
    17461 taaaacaagg tcaactttat ccagagccag aagctaacga acttagaaga aaattagctg
    17521 aaaagctaga tttaaaacca gaaaatttta ttgttgctaa tggtgcagat aacgttatca
    17581 ctctaatagg tgaggctttt ataaatagag gagatgaagt tatatattgt aacccaacat
    17641 ttccatcata tagaactgca actataaaaa atgaagggat accagtagaa gtacctttaa
    17701 cagaagatta taagtatgat ttacaaggta tattagataa gattacagat aaaacaaaat
    17761 taatatgtgt atgtaaccct aataatccaa caggaacaat tgtggatgat aaagagttag
    17821 aagaattttt aaagaaagtc cctgaaaata ttataactat attggatgaa gcatatatag
    17881 aatttttaac agttcctaat tatgtggatg gacttaagta tgtaagggaa aattataacg
    17941 ttatagtaac aagaactttc tctaaaatat atggtcttgc agggcttaga gtaggatatg
    18001 gtattgctaa agatgagata ataagaactt tatttacagt aaaagagcct ttctctgcaa
    18061 atcgtgttgc tataagtgga gcaacagcag cacttgatga tgaagagttt ataaaagaaa
    18121 catatgaatt aaatagagtt ggaatggctt actttaaaga agagtttaca aaaatgggat
    18181 ttgatgttgt agattcacaa tcaaatttcc tttatgtaga tatgaaaaca gatataccta
    18241 agttatttga agacttgaaa aaaagagggt ttgtaataag accaagtgct acacatgcac
    18301 gtgtaagtat tggaactatg gaagaaaata agaaatttgt agcagtttta aaggaaatat
    18361 taaatattaa aggagagcca agtattggat aatagataga aatattatga aacggggtgt
    18421 agatatgaac ttaaaaataa acgcagagag atgtaaaagt tgtgaatact gcgttatatc
    18481 ttgtaaaaaa ggagcattaa aaatatcaag taagataaac aaagaaggtt atgctcatgt
    18541 agaagttgat gagagtaaat gcatactttg tggaatttgc tatcaggtgt gtccagataa
    18601 tgtttttgaa ataattaaag aagaagcatg tcaagaggct tagaaggagt tgggataata
    18661 tgaaaaagat gtggaagggg aaccacgcca ttgcagaagc agccctaaga ggaggttgtg
    18721 aattttatgc aggatatcca attacaccac aaacagaggt aattgaattt ttatcacata
    18781 gaatgtctga acttggaaga acttttatac agtccgaaaa tgagatggct gctatatata
    18841 tggtatatgg agcatatgca agtggaatga ggtctatgac gagttcttca ggaccaggaa
    18901 tatctttaaa acaagaaggg atttcttact tatgtgcaaa tcattaccct tgtgtaatcg
    18961 taaatgtaca gagatggggg cctggtcttg gctcattgga ctcagcacag acagactatc
    19021 taagagatac tagaggggga ggaaatggag attatcattt gatagtttat gcaccaaaca
    19081 gtattcaaga gactgtagat ttgatgtata actcatttga tgtagcggag aagtacagag
    19141 tgcctgtcga aattttgaca gaagcagctt taggtcaaat gatggaacca gtagaatttc
    19201 cagagtttaa gaagagagaa ggagatttag gctggactta tgatggaagt aatagagacc
    19261 atgcaaaggt tgcagataac caaaagccaa ctttttgtat ggagaaacgt atgagaataa
    19321 ctgaaaatga acaacaatgg gaagattatc aagtagaaga tgcagaatat gtgtttgtag
    19381 cgttcggtat accatcaaga acaactatga atgctgttag aagacttaga gaacaaggtg
    19441 aaaaagttgg aataataaga ccaataacag tatggccttt cccatataaa gcatttgaaa
    19501 aggtaagtaa agatgtaaaa ggatttatct cagtagaatc aacagacaca ggtcaattag
    19561 tagaagatgt tgctttaacc tcaaagaaag tatgtaaaga aaatgtacct gtgtatggtt
    19621 tatttagtgg aaatcatata ccaaaaacat tgcaagtaat ggacacttat agcaaaataa
    19681 aatctggcga aatcaaggag gtgttctaat atggctacag aaaaattaac acctagcgtt
    19741 gtttcaatac caaacaagtt ttgcccaggt tgtggtcatg gaatagtaaa tcgtataata
    19801 gcagaggtaa tagaagaaca tggatatgaa aaaaatcatg tattaacatt aggagttggt
    19861 tgtgtctgca atatgaattt tagctggaat ggtgataaga tgcaaactgc acatggtaga
    19921 gcttcatcta cagctatagg agttaaagta gcacttccag atacattagt aatgacttac
    19981 caaggtgatg gagatgctta tgttataggt ctttctgaaa ctttaaatac agcatataga
    20041 aatcataatg tgactgtatt tgtaataaac aataataatt ttgcaatgac tggtggacaa
    20101 atgtcatgga ccactatgcc gggtcaagtc acaacaacaa gtgtaaatgg aagagatata
    20161 aagactactg gtagtccact gaaagttcct gagatggtgg caaacttctt tgatgtagca
    20221 tatgtagcaa gaggttcagt tcatagccca aaggaaataa ttaatctaaa aaaatatata
    20281 aaaaatgcaa tagaagctca attaaatgga gagggatatt ccttagttga aatattagca
    20341 ccttgtccaa ctaactgggg tgtatcacta gagaaatcta tcaaatggat ggaagaagaa
    20401 atagtgccat attatgctct tggcgaattt aagcaaaggg atggtgaata gttatggaaa
    20461 aagagctagt atttgcaggg tttggtggtc aaggagtttt aactcttggt ctgattgtag
    20521 ctgagtcagc acttgaattg gggaaacaag taacttggat gcctgcatat ggtccaacta
    20581 tgagaggtgg aaaagcctat agtgtagtaa agttttctga tgagagtata ggtgggcctg
    20641 atatggaaga aatagatgtt ttagtagcaa tgaataaacc atctttagat tatataaatt
    20701 tagttaaaga aggtggaaca gtagttatta atacatctgc aatagatgag aatgtaacat
    20761 taagagaaga tataagtgta gttaaaataa attgtcagga attagctcaa aaagtaaaca
    20821 atccaaaagc agcaaatata gtagtacttg gagcattaat tgctaaaaca gggttatttg
    20881 aaaaagaatt agctttaaaa actatgtgtg atttctttga ggaaaaagga aaaggaaagt
    20941 ttaatgttca aaatgaagca gcattcatgg aaggctataa taacgccatt tgataaatta
    21001 cccttatctt aagtgattta tctcaagatt taataatctt tagtgccaag agaaacttca
    21061 tcttgagata aaattcactt aaaatcacaa ctataaacat ataggagcgt gaatttatga
    21121 gtttggaaaa acttattaga ccaaaaagta ttgctatagt aggagtgact gataaacttg
    21181 gttttggaag aagtgcagca cttagtatag taaaaagtaa ggaaacagat agagtatatt
    21241 atgttaatcc caaaagagaa gaattgtttg ggagaaaatg ttataagacg atacaagaag
    21301 ttccagaagt agtagactgt gtagtagtat gtacacctag aaatgtagtt ccatctgtat
    21361 taaaagattc tggagagtta ggaacaaagg cagcagtggt atatgctagt ggatttgcag
    21421 aggaaggtac agaggaaggt acagatttag aaaatcaact tatagaaata tcaaatacat
    21481 acgatatgaa aatattgggt ccaaactgca tggggttgct aaattgtata gataaggtta
    21541 atctatgggc aggaggttct aaatgggatt tagatactaa aaaacctggt ataggaattg
    21601 taggtcaaag tggatttata actgctgaaa ttgtttcaag tgattatttt aatatatctt
    21661 atggtttttc aacaggaaat ggaaatatag ttacactaga agatgcagta gatttccttg
    21721 tagatgatga ttatgcatca gtaatagcaa tttaccttga aggtttaaaa aatcctcaaa
    21781 aatttataga tgctttaaaa agggcagcac aaaaaagaaa gccagtaata atattgaaat
    21841 caggaaggtc agaaaaaggt gctatttctg ctgcttctca tacaggaaat ttagctggtt
    21901 cgagtaaagc ttttgaaagt atttttgaaa aatatggagt aatttctgtg gaaaatttag
    21961 aacagtttat gtgtttagca caagctttta gtgttttaga tggtaattta cctgcaaatt
    22021 ctaattttgc agcaataaac ttctcaggag gagaaaatac tatttgtgca gatttagcag
    22081 aagaaaatgg tgtagaactt gcagaaatat cttctgaaac taaagaagaa atgaaaaaat
    22141 atcttccagg atttgcaaca cctaaaaatc cactagatgc tacaacagca ttattccatg
    22201 aaaaggatat gatagtaggt ttattacata cctttgaaaa ggatgatagt gttggattta
    22261 ctatggtagg tgcaaatata agagatgaag aaaatgaaat gcatgaaacc ctttgtaaag
    22321 ctgtaagtga agctagagaa caaggattga aaaaacctgt gtttgcagtt cctacacttg
    22381 aaggaaatag atatttagat tataggacta gattagaaga taatcaagtg cctataatgt
    22441 catcagttgg aactacattt acttgtttta ataaaatggc taagtttata gattatgatt
    22501 attcaaaaag aactttagaa tttaaagctg ttaagaaaag agagtcaaat aatgtagtag
    22561 cattatcaga attagatagt aagattgaga tgaaaaaata tggagtacca gttccaggac
    22621 aaggtaatgc aaaaagtatt gatgaattag atgaaatctt aaaatatatt aagtatcctg
    22681 ttgttttaaa aataaattct tctgaaatat tgcacaaatc tgatgttgga ggagttaaaa
    22741 taggaattga gaatagagat gaggcagtag atgcatataa tgagatttta actaatgtaa
    22801 aaaaagcaaa accagatgct aatattgatg gtatattagt tcaagaaatg gtagaaagtg
    22861 gtatagaaat tattattggt ataactaatg atgaccaatt tggaccaatg ttattagtcg
    22921 gtttaggagg agtatttgta gaggtattta aagatactac attatatcca ttacctataa
    22981 atcatgatga agctattatg atgcttaaaa aactaaaatc atttaagctt ttaaatggct
    23041 atagaggaag tgagccttgt gatattgatg ctcttgcaga tatgatggtc aagttaggaa
    23101 aatatgctta tgaaaataag gatgaagtca aggaaataga cttaaatcca gtgtttgtat
    23161 atcctaaagg aaaaggcgta tgtgcagtag atgccttaat agttaaatat aaatagttta
    23221 taagttttgg tattcaacca taattaaagc accgactgaa aatcacattt tgtaagttag
    23281 atagtgtgat tttcagtcac cccaaattta atttaaaaat attttaaaat gaaagtatag
    23341 ttttaagttt aattgtgaaa tagcactgta taaacaataa aatactacgt ataaaacgaa
    23401 taataaaagg tgttttaatg ataagaatca atcttgattt tcattaaaac accttcttta
    23461 ttatattaaa aatttttaaa cgttatattg tcacaggtgg gtttgttgga gctgcatatg
    23521 tacgattagc aaattcttgg tcaacaaaga atagaccacc ctctccagca agtttaacac
    23581 gttttatatt gtcttcacaa tttgtttctt cttctgcttg ttcagatata aaccaaagta
    23641 agaattgaga agttctaaag tctgattcag agtttgctac agctgctaag ttgtttatta
    23701 aagctgtaac ctttttttca tgagctaacg tacgctcaag aatatccatt gcagaagtaa
    23761 agtctgaatc tggcattggg atagcatcaa gctgaacctt tcctcctaca ttatgtaagt
    23821 atttgtaaaa aaacatagcg tggtctgtct cttctttata ttgaacataa aaccaattag
    23881 aaaatccttt aagtccttct gcttcaaggt aagaagacat agaaagataa agatacgcag
    23941 aatataattc atggttaatc tgctcattaa gtaatttttc cattttagct gaaatcattt
    24001 ttatacctcc aaattttaaa tgctcttata aaatataata atattaaatg gaaaaatatt
    24061 caataaaagt gtttaaatat tttacatatt taataaaaat ttatactacc attaaaaaaa
    24121 ttataactta caatggctat agagccaatt aatggtgcat ttgctccaaa atttgaatgc
    24181 cttacactta tttttttata tttagaaaaa cttgctgaag cagtcaattt ttttaataat
    24241 gtattttcta taaatgtacc aggtatatta aagtcatagc ctattattat agaactacaa
    24301 tcaatcaatg taagtgtatt aactaatgca tatgatacgt atgtacaaaa ttcatcaagc
    24361 aaaaacatgg caagactatc tttattattt gcctcatcta ttaacttgac taaagaaaaa
    24421 tctttacagt taattaatgg ggagttagga taaatatggc tcaatgattc aattctttta
    24481 attagattac tgacacttgt ataataatct aaacatccat tattaccaca actgcacaat
    24541 ggtccactaa aattaattga tgtatgtcct atttctccac tttgtcctag gttgccagta
    24601 tgaagtttat tttctagaac tagaccagct ccaataccat tcattatgtg taggtatata
    24661 taatttgata tatttttacc taaaccatac attttttctg ctaatgcacc cgaattagca
    24721 tcatttatca aaaatattgg caaatcagac atttctttta taaaatctac tatatttagg
    24781 tttgatacat ttccaaaatc tgggggattt agtatggtac catttatatc atctactgga
    24841 cctattgaag aaataccaat tccaattata tctctcttgt tgattttcat tattttagta
    24901 aacattcttt ttattatatc ttttaaaaca tcatttgata gacatttggg gtaagtttca
    24961 gaaagttgtt ttacaatatt accttttaaa tctgaaataa ttaccttaca tatatttctt
    25021 cttattagga ttccacaaat acaaggcgat atattagaaa tatctaataa aataggttta
    25081 cgacctgatg aatgtgcatt taaagttgat gactctattt ccctaatcat attttcatta
    25141 ataagttcag taactagatt tgataaagtc attttagtaa gtcctgtaga cttagctaaa
    25201 tcaactctgg acataggtgt atttgtagaa agtaatttca atattattaa cttattttta
    25261 atttttacat ctttgatgtt agaaccttgt ttgttactca tcataaatct ccttaaataa
    25321 agttaagtgt tttatttatt ttcaagtata ctatcacctt ataatagagt aacatttttt
    25381 tacttgaaaa tatcattaat tagaatatat cacattatat atcataaaat atataaaaaa
    25441 atgttagaat taaattaaaa gtgaaaagta gattgatata taaatagtaa atacttaatt
    25501 aaaaaaagat aatgatagca ataattattg ttaccaagaa tctactactt caagtgttac
    25561 cttaggtatt taagttgata gtggtttgca cgtaataaac ttgatgtaat atat
//
LOCUS       DAEPIN010000058        23893 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.058, whole genome shotgun sequence.
ACCESSION   DAEPIN010000058 DAEPIN010000000
VERSION     DAEPIN010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23893
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.058"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            145..486
                     /locus_tag="KR250_002670"
     CDS             145..486
                     /locus_tag="KR250_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator"
                     /protein_id="HBH0068592.1"
                     /translation="MKEENFINVEYEDKRFSINSKEVLYIEKQEYEVKIYTESGNYEV
                     GENAPCIRKLLKEENFFKCSNSYIVNLDKCEFYLSNNCIFVNNNFIRILGSERKKKLK
                     KKLEKLYDYED"
     gene            637..1050
                     /locus_tag="KR250_002671"
     CDS             637..1050
                     /locus_tag="KR250_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1653 domain-containing protein"
                     /protein_id="HBH0068593.1"
                     /translation="MLELKNNLGISERKLKYPCIYKHFKGKYYATMGLSKAIDHIENI
                     CEIYGKENLIQNRNKYKLVIRHTEREEDIYVYRDLDGNFYHKKEEDTNDLVLYKTLYD
                     DTGIFARPLDMFLEKVDTDKYVNSIQEYRFEEVYK"
     gene            1252..1893
                     /locus_tag="KR250_002672"
     CDS             1252..1893
                     /locus_tag="KR250_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBH0068594.1"
                     /translation="MGNTFYIVRHGQTDWNILGKTQGHGNSDLTPQGIEQAKELSEDI
                     GKYSIDYIFSSDLGRAMQTAQILGDKLNIEVQKTEALREMGFGVWEGLLIKEIQKDYS
                     DIYATWRNEPHLVNIPEGETLKIIKERVDAFIKELNEKYDNKNIILVTHSITLRVMLL
                     SFLESGMENIYRIKQDNTALNIVEFKDYGPVIVKMNDTSHIKNHVKINNSALE"
     gene            1917..3170
                     /locus_tag="KR250_002673"
     CDS             1917..3170
                     /locus_tag="KR250_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBH0068595.1"
                     /translation="MSKVIVVGGGASGMMAALSASKNNNEVILVERNGELGRKLRATG
                     GGRCNFTNNREIEDFFDKVVSNKKFLYSSFYTFTNKDLISYFESRNLEYKIEEENDHK
                     VYTKNDKSIEVIEVLNKDLLNHNVKIMYNKKVIDIITEEIALKDDSNKDKSKYLIKGI
                     ILDNGDKILGDKVIISTGGVSYSKTGSDGSMYKILKKHGHTLNKLYPALVPLTIEEKW
                     IKDLQGISMKNVEISCKIKKRKISKSGDMLFAHFGITGPCVLIMSSYINKIIEKEKVE
                     LNIDFLPNLSTDEISSIIRAFPNKNVLNNLKQILPQNFLKEIFSLLSLVDKKASDLSK
                     ADEIRIIEYIKNMKLTCNGTTGINTGMVTSGGISVKEINSSTMESKLVKNLFFTGEVI
                     DIDAETGGYNLQIAFSTGYLAGISV"
     gene            3216..3866
                     /locus_tag="KR250_002674"
     CDS             3216..3866
                     /locus_tag="KR250_002674"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HBH0068596.1"
                     /translation="MGNLVIAVDGPAGAGKSTIAKIVAKKLNINYIDTGAMYRAVTYK
                     CLKSGIDVNNEKEVIQIAENSDIDFKDNNIYLDKEVINEEIRTIEVSNNVSNVAKIKE
                     VRQLMVEVQRKIGMKNSVILDGRDIGSYVFPDADYKFFLVATPEERGNRRYKELCNKG
                     YNTTLEEVIEDIIRRDEIDSNREFAPLVKANDALEIDTTGKTIEEVVEEVVSKINL"
     gene            3917..4513
                     /locus_tag="KR250_002675"
     CDS             3917..4513
                     /locus_tag="KR250_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HBH0068597.1"
                     /translation="MNFYRFVINIFKGFSKIFFKYEVIGAENIPDRGNIVIASNHKSN
                     LDPIFLAAAIENREIAAIAKKELFKVKPLGFILKKLHVMPINREKPDVSTIKTILRSV
                     RDGYVLGIFPEGTRIKGDSFGKAKAGLSVFTIKSKSKVVPVSIISKYKLFSKVIVYIG
                     EPISFEEHFKEKLSNDDHERISQEILEVIKQNYFKYSK"
     gene            4526..5365
                     /locus_tag="KR250_002676"
     CDS             4526..5365
                     /locus_tag="KR250_002676"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HBH0068598.1"
                     /translation="MNIKIAKNAGFCFGVKRAMKMAWDEVEKNDSGIYALGPLIHNKQ
                     AVAKYEEKGLKTVNEIDTIPNHENMIIRSHGVPENIYKEAKDKKLKIVDTTCPFVKKI
                     HTVVSEYHNKGYEIIVIGDMKHPEVIGINGWCENSAIIIKTLEQMENMEFDNSKRYCL
                     VAQTTINPELYISIVNKLSDKLEEIVFNDTICSATKTRQESAKELAKKVDCMIVIGGK
                     HSSNTQKLVKVCEDLVPTFAIETKDELDVNTLKKYKNLGITAGASTPNWIIEEVVTFL
                     ENL"
     gene            5610..6764
                     /locus_tag="KR250_002677"
     CDS             5610..6764
                     /locus_tag="KR250_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic efflux protein"
                     /protein_id="HBH0068599.1"
                     /translation="MEMLISASEEAFLHVGSMIGFFILLFGYINYKTSGNFTNIISKN
                     RKFQPLIGALIGAIPGCGGSLAIMPLYINGKLSFGAIIASLIASMGDAAFVLISSNIK
                     MYFFVTIVSTITGIITGQLVDYFKLEEKLGLKNRRKSDKYDISKNNPKENKEQHNHDE
                     IILDTLAKSHGNTNRLAFIITHGKGYKIYIGIIVIGFIFMSLAHSGLNLPIIEKLHSL
                     EEVIAVIGILFSIIYMWCFKKVFKNSNQKEEENKKISLREMLIHSVGEISFVITWIFI
                     AYLIYDLIILILGGDEYLVKLVLSTGVISVFIGAGLGLIPGCGIQIVLMSFYLKGTIP
                     LGAVIANSISQDGDALFPLLAMDKKSSLWAMIITTIPAILVGMIVYAFLG"
     gene            7070..7246
                     /locus_tag="KR250_002678"
                     /pseudo
     CDS             7070..7246
                     /locus_tag="KR250_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728625.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
     gene            7895..9013
                     /locus_tag="KR250_002679"
     CDS             7895..9013
                     /locus_tag="KR250_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBH0068600.1"
                     /translation="MIVVEKAYKFRMYPNKKQQELINKTFGCCRFVYNKYLAKRIDVY
                     KKHKETFTYKQCSSDLTNLKKELKWLKEPDKFSLQNALRDLDNAYKKFFKEKAGFPKF
                     KSKKINRFSYKTNFTNGNIMYCGQYIKLPKLGMVKIRDKQVPQGRILNATISKEPSGR
                     YYVSLCCTDVDIGAFENTNNHIGLDLGIKEFCISSCGDFIENPKYLKKSLNKLAKLQR
                     ELSRKTIGSLNRNKARLKVARFQEHIANQRKDFLQKLSTKLIKENDIICIEDLQVKNM
                     IRNRKLSRLISDVSWSEFIRQLEYKANWHGRQIVKVGKFFASSQICNKCGYKNEEVKD
                     LNIREWICPSCNETHDRDINASINILKEGLRLITIQNK"
     gene            9146..10651
                     /gene="ade"
                     /locus_tag="KR250_002680"
     CDS             9146..10651
                     /gene="ade"
                     /locus_tag="KR250_002680"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HBH0068601.1"
                     /translation="MSPGFIDAHMHIESTMVMPIELSKKLLKSGTTTIIADPHELVNV
                     KGVSAIDFLLESTKDIPLNVYIMVPSSVPATSFETNGVGKFSAKDMESYVNNPRILGL
                     GEVMCFNDVINSENEILDKLELFKNKVVDGHAPNINGKSLQTYVCAGIENDHECITFD
                     EVYEKLRAGLKILIREGSAAKNLKSIVSGMLKHNLPIEEFMFCTDDKHLDDIEKQGHI
                     RWNIKCAIDLGMEPVRAIKVATYNSAKAYGLRKIGAIGAGYKADIVVLNDLDKMEVDS
                     VYKDGNLVNEEMFSNYNYEIKDKELLNTVKFKYINKEKIQLKVSEKNYVMEIVPYQIL
                     TNKVYESLPCADGYFVPNKEYSKLCVVERHRMTGNVAVAPLKGFGIKNGAIATTVAHD
                     SHNIIVAGDNDDDILVAINYLKEIQGGYVIVSNGKVLAHLSLQVAGLISTFTAEEVQE
                     ITDNMLEIARKMGVPEYVDPFITLSFMALPVVPQIRLTDLGLFDVEEFKFI"
     gene            11003..11467
                     /gene="bcp"
                     /locus_tag="KR250_002681"
     CDS             11003..11467
                     /gene="bcp"
                     /locus_tag="KR250_002681"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HBH0068602.1"
                     /translation="MLSIGTKAPEFTLEDKDGNKVSMSDFKGKKVVVYFYPKDNTPGC
                     TRQACAFRNAYDGFKKEDIQVIGISKDSIKSHQKFAEKHELPFILLSDPDLVAIKAFD
                     VWKEKKMYGKTALGVVRATYIIDENGIIEKVFEKAKPDTNAQEILEYLEKQE"
     gene            11477..12220
                     /gene="yaaA"
                     /locus_tag="KR250_002682"
     CDS             11477..12220
                     /gene="yaaA"
                     /locus_tag="KR250_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HBH0068603.1"
                     /translation="MIIMLSPAKNMKNIEVFDRDLSLPCFIDNTKEIVENIKTFGIED
                     FKNKMKINEKLAVLNKNRFESIKFDRLGNPAILTYDGIQYKNIEAENFTRKDEEFANS
                     CIRIISGLYGVVKPYDSIYEYRLEMQTKLRVGEFKNLYEYWGNRIYKELIKEKTAIVN
                     LSSNEYSKSIEKFIKDSDTYITCTFKVNKNGILKVESTQAKKARGMMTKYIVKNRIRD
                     IEELKKFNLEGYKYKENLSNNSEYIFVKE"
     gene            12348..15005
                     /locus_tag="KR250_002683"
     CDS             12348..15005
                     /locus_tag="KR250_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBH0068604.1"
                     /translation="MENHKQMPWHSRATHEILESLDSSEEGLTDTEAEKRLKENGLNE
                     LRKKPPRTTLQMLWSQITDAMVMILIGAAILSLIFGEFTEAFVILIIVTVNAVIGIVQ
                     EKKAESSLEALKNMNSPTARVMRDGEENVIPASNLVVGDIVFLEDGAMVPADLRLIET
                     SSLKIQEASLTGESVPSEKDADTILPKECVLGDRSNMAYTSSIVTYGRGVGVVVATGM
                     DTEVGNIAHLLDSQDDFDTPIKRKLNTVGKTLSVIGIIVCVVIFAIGAFYQRPLLPMF
                     MIAISLAISIIPEGLPATATIVMALGVQRMAKRNALIRKLPAVETLGGATVICCDKTG
                     TLTLNKMTVTHIAVNGDFESGKVTSIDTASEKHPLVYKEIVYAGALCNDACINPDKTD
                     EILGDPTEGALIFMAKKFGIDQEALEEEYPRVFEQPFDSDRKCMTTVHNIDEKIIAYT
                     KGAVDEILDLCTKILTSKGERNITEVDKKNIHELCLNMSKDALRVLGFAKREISSIPK
                     EDSENIEYDLTFIGMVGMIDPPRTEVIDAVSTCKEAGIRTIMITGDHKVTATTIAHEL
                     GIWSEENTVISGDELDNLSDDELDEAVKNTTVFARVSPFDKLRIIQSLKRIGEVPAMT
                     GDGVNDSPALKSADIGIAMGISGTDVAKDSSDMILMDDSFTTIAYAIKEGRRVYRNIQ
                     KVIQFLLVGNIAEILTLFVAILLNWDTPLLAVHILWVNLATATLPALALGVDPPSRNI
                     MKHQPVKSTTLFEKNLIQRVVTQGIFVAIMTILAYKIGLNIENHSVGQTMAFCVLAFS
                     QMLRAFSQRSNTDSMFNRNNGKNPFLLISFLTSAFLIAVILFAPVFRNAFNLTVLNNL
                     EWLITIVLAIMSVIQVEVGKIIKRKKLKK"
     regulatory      15059..15161
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            15271..16536
                     /locus_tag="KR250_002684"
     CDS             15271..16536
                     /locus_tag="KR250_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HBH0068605.1"
                     /translation="MYNKETICVQGNYKPGNGEPRVLPLYQSTTFKYSSIDQLAELFD
                     LKVDGHIYSRISNPTIQAFEEKISLLEGGVSSVAVSSGQSANMLAVLNICKSGDSILC
                     SSKVYGGTFNLLGPSLKKFGIDLISFDLDSSEDEIVELAKENTKVVFAETLANPTLEV
                     IDFEKIANVAKRINVPFIVDNSLASPVLCNPLKYGANIVTHSTTKYLDGHASSVGGII
                     VDGGNFNWDNGKFPELVEPDPTYHGISYTQKFGNAAYATKARVQLLRDYGNCLSPFNA
                     YLTNLNVETLHLRMERHSENALKIARFLEKHENVDWINYPGLEDNKYYENAKKYLSRG
                     CSGVLSFGVRGGLENAKKFVEKLQIASLVTHVSDVRTCVIHPASTTHRQLTEEQLIAS
                     GVLPSLIRLSVGIENVEDLIADLNQALNF"
     gene            16566..17456
                     /gene="metA"
                     /locus_tag="KR250_002685"
     CDS             16566..17456
                     /gene="metA"
                     /locus_tag="KR250_002685"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HBH0068606.1"
                     /translation="MALILPKGLPVINKLLDEGIDVIYKEDFKKELKYEENIDTKIAI
                     LNLMPIKIDTELDLLRRIDKTGFNVSVEFIKISTRESKRSCNEYVKEFYKTFDEAKGE
                     YFDGFIITGAPVEQMEFEEVDYWNELEEIMDYSKRKTKSTLYICWAAQASLYKYYNVK
                     KLPLSQKCFGVFKHKVDKDSKIVDGFENEFFAPHSRHTTVNIEALKENKELSIVSHSK
                     EAGPYIITNSRDVFVMGHSEYDKYTLDKEYKRDINKGDKISIPQNYYINDDPSEEPTV
                     KWKKHSELLFRNWIKNYLIQ"
     gene            17944..18387
                     /locus_tag="KR250_002686"
     CDS             17944..18387
                     /locus_tag="KR250_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBH0068607.1"
                     /translation="MDEMSFKKELLELTRDINMKFTTLLSDFYQPLGITAVQALILSE
                     LCEHGEKKISDLGKNLNMTNSNVSVICQRLEKNGFLNRIRDIEDQRIVKVKVTNKSLD
                     IQEHISSSIFDSYFENMTSEKLQDMEDIIEGLEKLNKLLTYIDCK"
     gene            18418..20235
                     /gene="ftsH"
                     /locus_tag="KR250_002687"
     CDS             18418..20235
                     /gene="ftsH"
                     /locus_tag="KR250_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HBH0068608.1"
                     /translation="MDEHKPQKRSALYYYTVIIIVVMFLNALIFPSLMDKKMVEIDYG
                     KFLTMLDTGKVKSVEIENNRIAINPSSSKDKNIYITGRMDDPELVDRLKNAKVEFTKV
                     IPKENSPLLSILLTWIIPIGILMLFGNLMMKSMQKRMGGNAMQFGKSNAKMYVSAQSG
                     KRFNDVAGQDEAKEALTEIVDFLHNPEKYKKIGAQMPKGALLVGPPGTGKTLLAKAVA
                     GEANVPFFSISGSEFVEMFVGMGASRIRDLFKQAKEKAPCIVFIDEIDTIGKKRDNGS
                     GLGGNDEREQTLNQLLSEMDGFDGGIGVVILAATNRPDSLDKALLRPGRFDRRIPVEL
                     PDLGGREAILKVHAQNVNVSSDIDYNAIARSTSGASGAELANIVNEAALLAVKSGRDI
                     VEQQDFDESVETVIAGYQRKGAVISDKEKKIIAYHEIGHALVAAMQKHSAPVHKITII
                     PRTSGALGYTMQVAEDESVLMSKEEAIDKITTFTGGRAAEEVIFNTCTSGASNDIEQA
                     TKIARAMVTRLGMSEEFDMVALETVSNQYLGGDTSLACSSDTAAKIDEEVFKIVKACH
                     KNAIEILENNIDKLHELANYLLEKETITGDEFMEILNLN"
     gene            20413..23115
                     /locus_tag="KR250_002688"
     CDS             20413..23115
                     /locus_tag="KR250_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
                     /protein_id="HBH0068609.1"
                     /translation="MDRPNPDILLEKIKNEEEKLSRGQLKIFFGYAAGVGKTYSMLES
                     AQNLKKVGVDVVVGYIEPHTRPETLALLDGLEILPVKEIEYKSIKLKEFDLDAALARK
                     PEVILVDEFAHSNVAGLRHTKRWQDIEELLLAGINVYTTVNVQHLESLNDIVEIITNV
                     SVKETIPDKFLDTNTQLELIDVEPDVLLERFNEGKIYKKEQATRAKNNFFIKDNLVAL
                     REIALRKTAERVNKEVQMTRLSKGDVTVIPTSDTLLACISPSPSSAKVIRTASRISDS
                     TFAKWIALYVETPNTARLPFDEQKQLQNNLKLAKKLGGEIIVLHGENIIEQILRIAKL
                     RNVTKIVIGRNHSSNKRFSKRFKKDIVDKLIDEVDYIDIHIIPYKFASDVKYKPKKDK
                     SSIKSKFKISKVDFIKLIFITLMISILAYAVQSMGFIRENILLIYMLGVVLVSMWTKG
                     YSTGIISSVFNIVLLNYFFTAPLYTLSIADSNYIVTLVVFSIVGIITSTLTSKIQHEA
                     ETAAKREENTKMIYQIIKGFLRLSNKDDIVNKGIELLSLSLSRDVICYLNVDKHNKSK
                     LYKKNTNNKDKNDLDSEDEKAVASWVLSNSTVAGNDTDTLPGSKGYYIPIVGMNLTLG
                     VVGISCIDSKLDTEDISLIETIIAQMAIALDREILSEAKENTNLEIERERLRSNLLRA
                     VSHDLRTPLAGISGAVSTIIKNKGTIGQDIIDELLSGVYEDTQWLIRLVENLLSMTKI
                     DEGKLEVKKHSELVEEIISEALQKIKKRIENALIDINIPEQILFVPMDAKLIEQVLIN
                     LIDNSLKYSKEDCKIEINVYEKDDYVWFEVSDNGPGISKELKKHIFDRFFTGEEGAKD
                     SRKGVGLGLSICKSIIQAHKGEIMVENNKDKGSTFKFSLPKENE"
     gene            23149..23847
                     /locus_tag="KR250_002689"
     CDS             23149..23847
                     /locus_tag="KR250_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0068610.1"
                     /translation="MSKKLILLVEDDKTIRKFISTALLTQDYDVKEAITGKEGISIAV
                     SYSPDVVLLDLGLEDMDGIEVIKAIRQFSNIPIIVVSAREQDRDKVEVFDAGADDYLT
                     KPFSIVELLARVRVAFRHSQVEAQQKDDVKSTFEVDKLLIDFDKRKVIVDDVEVHLTP
                     IEYNILSLLAKYHGKVLTHNFIIKEIWGSVIGNETKSLRVFMATLRRKIEKQPANPRY
                     IITEVGVGYRLNDE"
BASE COUNT         9590 a         2586 c         4291 g         7426 t
ORIGIN      
        1 aatgttaaaa aataaactta tacattaata ttaataataa ttatttaaat atcttaagta
       61 aatcagtctt acatatattt ccgatagatt atattgataa aaaacactaa aattaaatta
      121 ataaaagtac ttggagggat ggaaatgaaa gaggaaaatt ttattaatgt tgaatacgag
      181 gataaacgtt tttcaattaa tagtaaagaa gttttgtaca tagaaaagca agagtatgaa
      241 gtaaagattt atactgaaag tggtaattat gaagttggag aaaatgctcc ttgtataaga
      301 aaactattga aagaagaaaa ttttttcaaa tgcagtaata gttatattgt aaatctagac
      361 aagtgtgaat tttatctcag caataattgt atttttgtga ataataattt tataagaatt
      421 ttaggttcag aaagaaaaaa gaaattaaaa aagaaattag aaaaattata tgattatgaa
      481 gattaatatt gggaggctgt attttataca gcctctttat ttattgattg tattattaat
      541 aaattttaaa ttatgataaa atataaaaca agtaacttat ttcagaataa aatacttttt
      601 aaaagaaagg gacgagatat gggagattat aataaaatgc ttgaattaaa gaataactta
      661 ggtatcagtg aaagaaagtt aaaatatcct tgtatctata aacatttcaa agggaaatat
      721 tatgctacaa tgggtcttag caaggctatt gatcatatag aaaacatatg tgaaatatat
      781 ggtaaagaaa atttaatcca aaacagaaat aaatataaat tagttattag acatactgaa
      841 agagaagaag atatctatgt ttatagggat ttagatggaa acttttatca taaaaaagaa
      901 gaagatacta atgatttagt tttatacaag accttatatg atgatacagg aatatttgca
      961 agacctttgg atatgttttt agaaaaagta gatacagata agtatgtcaa ttcaattcaa
     1021 gaatacagat ttgaagaagt atataaataa tataagagaa atatgtttat aaatttagaa
     1081 aaattcttta acagtacaat aatttgcaga aatacaaatg agatttattg taaataatat
     1141 gttagattag attataaaat agaaacaaag taaagtatta tatgatataa ttaagttttg
     1201 agaagtgata atttatattt aataaaaaat aggcatatat taggaggaca tatgggaaat
     1261 actttttata tagtaagaca tggacaaacg gattggaata tattgggaaa aacacaaggt
     1321 catggtaatt ctgaccttac gcctcaaggt atagaacagg caaaagaact ttctgaagat
     1381 attggcaagt attcaattga ttatattttt agtagcgacc ttgggagagc aatgcaaaca
     1441 gctcaaattt taggagataa gctcaatatt gaagttcaaa aaactgaggc acttagagaa
     1501 atgggattcg gagtttggga aggcctttta ataaaagaaa ttcaaaaaga ttattcagat
     1561 atatatgcaa catggagaaa tgaaccacac ttagtaaata taccagaagg cgaaactctt
     1621 aagattataa aggaaagagt agatgctttt ataaaggaac taaatgaaaa atatgataat
     1681 aaaaatataa ttcttgttac acattcaatt acattgaggg ttatgttgtt gtcattttta
     1741 gaatcaggta tggaaaatat atatagaatt aagcaagata atactgcttt aaatatagta
     1801 gaatttaaag attatgggcc tgttatagta aaaatgaatg atacaagtca tataaaaaat
     1861 catgtaaaga taaataattc tgcactggag taggaatcaa ttataaggag aaatttatga
     1921 gtaaagttat agtagttggt ggaggagctt ctggaatgat ggcagctttg tcagcttcaa
     1981 aaaacaataa tgaagtaata ttagttgaga gaaatggaga attaggaaga aaacttaggg
     2041 caactggtgg cggtagatgc aattttacca ataatagaga gatagaagat ttttttgata
     2101 aagttgtcag taataaaaaa tttctctata gtagtttcta tacatttaca aataaagacc
     2161 ttatttcgta ttttgaaagt agaaatttgg aatataagat tgaagaagaa aatgaccaca
     2221 aagtatacac taaaaatgat aaatctatag aagtaataga agtattaaat aaagatttac
     2281 tcaatcacaa tgtaaagata atgtataata agaaagttat agatattata actgaagaaa
     2341 ttgctctaaa agatgattct aataaggaca agtcaaagta tctaataaaa gggattattt
     2401 tagataatgg agataaaata ctaggagaca aggttattat atcaactgga ggagtgagtt
     2461 attcaaagac gggttcagat ggttcaatgt ataagattct taaaaagcat ggacatacat
     2521 taaacaaact atatcctgct ttagttccac ttacaataga ggaaaaatgg attaaagatt
     2581 tacaaggaat atccatgaag aatgtggaaa tatcttgtaa gattaagaaa aggaaaatat
     2641 caaaatctgg agacatgcta tttgcccatt ttgggataac aggaccttgt gttcttataa
     2701 tgtcttctta tattaacaag attattgaaa aggaaaaggt agaattaaat atagatttct
     2761 taccaaattt aagtactgat gaaatctcta gtataatcag agcatttcca aataaaaatg
     2821 ttctaaataa tcttaagcaa atactacctc aaaatttctt aaaagagatt ttttctctat
     2881 tatctttagt tgataaaaag gcaagtgatt tgtctaaggc agatgaaatt agaattattg
     2941 agtatataaa aaatatgaag ctaacttgta atgggactac aggtataaac actggtatgg
     3001 ttacttctgg tggtatttca gttaaagaaa ttaattcttc tactatggaa tctaaattag
     3061 ttaagaactt attttttaca ggtgaagtaa ttgatataga tgcagaaact ggtggatata
     3121 atttacaaat agcattttca actggatatc ttgcgggtat aagtgtataa atataatttt
     3181 ttaataatta aatatgaata aatagaggtg tatatatggg taatttagtt atagctgtag
     3241 atggtccagc aggagctgga aaaagtacaa tagccaagat agtagcaaaa aagctaaata
     3301 taaattatat agatacaggt gctatgtaca gagcagttac ttataagtgt ttaaaaagtg
     3361 gtattgatgt caataatgaa aaagaagtta ttcaaatagc tgaaaattcg gatatagatt
     3421 ttaaagataa caatatatat ttggacaaag aagttataaa tgaagaaata agaacaatag
     3481 aagtaagcaa taatgtttct aatgtagcaa aaataaaaga agtaagacag ttgatggttg
     3541 aagttcaacg aaaaatagga atgaaaaatt cagtaatact agatggtaga gatataggtt
     3601 catatgtttt cccagatgca gattacaaat tctttttagt agctactcca gaagaaagag
     3661 gaaatagacg ctataaagaa ctttgtaata aaggatataa tactacttta gaagaagtaa
     3721 ttgaggatat aataagacga gatgaaattg attcaaatag agaatttgca ccattggtaa
     3781 aagctaatga tgcactagaa atagatacta caggaaaaac tatagaagaa gttgtagaag
     3841 aagttgtatc taaaataaat ctttagtttt ttatgcatca taatgaaaat aaatgtagtg
     3901 ttgaagggag ataaacgtga atttttatag atttgtaata aatattttta agggtttttc
     3961 taaaatattt tttaaatatg aagttatagg tgcagagaat attccagata gaggaaatat
     4021 agtaatagca tcaaatcata aatcgaatct tgaccctata ttcttggctg ctgccataga
     4081 aaatagagaa atagctgcaa ttgcaaagaa agaattgttt aaagtaaaac cattgggatt
     4141 tatattaaaa aaattgcatg ttatgcctat aaatagagaa aaaccagatg tatctacaat
     4201 aaaaactatt ttaaggtcag ttagagacgg ttatgtatta ggaatatttc cagagggtac
     4261 tagaataaaa ggagattctt ttggtaaggc taaggcaggt ctttctgtat ttacaataaa
     4321 aagtaaatct aaagttgttc cagtatcaat aatatctaaa tataaacttt ttagtaaagt
     4381 aattgtatat attggagaac caatatcatt tgaagaacat tttaaggaaa aattgagtaa
     4441 tgatgaccat gaaaggatat cacaagagat tttagaagtg ataaaacaaa attactttaa
     4501 atattcaaaa taagggggta aagttatgaa cataaaaata gcaaaaaatg ctggcttttg
     4561 ttttggagta aaaagagcaa tgaaaatggc atgggatgaa gttgaaaaaa atgattctgg
     4621 catatatgct ttaggtcctc ttattcataa caagcaagca gtagcaaaat atgaagaaaa
     4681 aggtcttaag actgtgaatg aaattgatac aataccaaat catgaaaata tgataataag
     4741 gtctcatgga gttcctgaaa atatatataa ggaagccaaa gataaaaaat taaaaattgt
     4801 ggatactaca tgtccatttg taaagaaaat acatacagta gtaagtgaat atcataataa
     4861 aggctatgaa ataatagtta ttggagatat gaagcatcca gaagttatag gtataaatgg
     4921 atggtgtgaa aattcagcta taataataaa gaccttagaa caaatggaaa atatggaatt
     4981 tgataactcc aaaagatatt gtcttgttgc acagactaca ataaatccag agttgtatat
     5041 aagtatagtt aataaactat cagataaatt agaagaaata gtatttaatg atacaatttg
     5101 ttcagctaca aaaacaagac aagaatcagc taaagagtta gctaaaaaag ttgattgtat
     5161 gatagttata ggaggtaaac acagttcaaa tactcaaaag ttagtaaagg tttgtgaaga
     5221 cttagtacca acttttgcaa tagagactaa agatgaattg gatgtaaata cgcttaaaaa
     5281 atataaaaat ttgggtatta cagcaggggc atctacacct aattggataa tagaagaagt
     5341 tgtaacattc cttgaaaatt tataatttga taggtaataa aaataggtgg acattgttat
     5401 aatttatcat aatatccacc tttgatttca tattacatgt ggtaaataga taacctaata
     5461 tcaaaaagtg tgtatcttaa gatgaaaaac taagagtata agatgcgcac tttttaatat
     5521 tagaaattca aaagaaatat tgatattgat tatcaataga aaatagaata taatataatt
     5581 gttgcaaaat tagtctagga ggtaaattaa tggaaatgtt gatatccgct tctgaagagg
     5641 cgtttttaca tgtaggttct atgattggat tttttatttt gttatttgga tatattaatt
     5701 ataaaactag tggaaatttt actaatatca tatcaaaaaa cagaaaattt caacccttaa
     5761 taggtgcttt aattggagct ataccaggtt gtggcggaag tttggcaata atgccattgt
     5821 atataaatgg aaaattaagc tttggagcaa taattgcaag tttaatagct agtatgggag
     5881 atgcagcatt tgtacttatt tcatcaaata ttaaaatgta tttttttgtc actatagtta
     5941 gtacaattac aggaataata actggacaat tagtggatta ctttaaacta gaagaaaaat
     6001 tagggttaaa aaaccgtaga aaatcagata aatatgatat aagtaaaaat aaccctaaag
     6061 aaaataaaga acaacataat catgatgaaa taattttaga cactctagct aaatctcatg
     6121 gaaatacaaa tagacttgca tttataataa cacatggaaa gggatataaa atttatatag
     6181 gaattatagt aatcggtttt atatttatgt cacttgctca ctctggactt aatcttccta
     6241 taattgagaa gttacattca ttggaagaag taatagcagt gattggaata ttattttcaa
     6301 taatttatat gtggtgtttt aaaaaggtat ttaaaaattc taatcaaaaa gaggaagaaa
     6361 ataaaaagat ttctttgcga gaaatgctta tccattctgt tggagaaata tcatttgtaa
     6421 ttacatggat ttttattgca tatcttattt acgatttgat aatattaatt ttaggtggag
     6481 atgaatactt agttaaatta gtattatcaa caggtgtaat aagtgtattt ataggtgcag
     6541 gtcttggact tattccaggt tgtgggatac aaattgtgct tatgtcattt tatttgaagg
     6601 gaactatacc acttggtgca gtgatagcca actctatttc tcaagatgga gatgcacttt
     6661 tcccactttt agcaatggat aaaaaatctt ctttatgggc tatgattatt actactatac
     6721 cagcaatact tgtaggtatg atagtttatg catttttagg ttaaatataa ttatgaataa
     6781 agaagtatta aaaaaataat aaagcacata aatttattta taagaatagt gaagattaaa
     6841 tataaaaata aaaaattcaa tatgaattaa attattgctt aaatattaat gagaacatgg
     6901 gagataatat tattcctcat gttctctcta tttaattaaa ggaattatgg aacaaatttc
     6961 aagtgatact ttctatcaaa actcatatat aatatatatt aataaattaa actataatac
     7021 gtaatacagt cagtatattg atataaaaaa cttaggagga gttgtaaaaa tgggtaattt
     7081 aaaaagacag tgtgatgttt caagtggaag agaaaaagct gacattgtac taaaaaatgg
     7141 gacaatcata aatgtattta cagaagagtt aataacagga gatgtagcaa tagtaggaga
     7201 cactatcgtt ggaattggcg attacaaggg caatgtagaa attgattaaa aaatagctac
     7261 gcacactcgt gactttagtc atgagttagt agctaaaata gtcagcatat agggaaacct
     7321 atatgtagag ataggataga acctatccaa tactacttga attgctggaa acccctaaag
     7381 ctaatcaaac taaaacataa ggatgaaata aacctaagtg tgaaagttac gaaagtagaa
     7441 aaaattggtt agatggtata aggttaaacc ctaagtactt taaaatgggc aatcagcagg
     7501 taaactccga ataggagaaa cttcaacgac tattcctctt gagggaagta cactacaagc
     7561 tattggtagt ggaagtgggt agaccttaac ggataatgct gaaggataag atatagtctg
     7621 tgcttgcatg aaagtgtaag aagttcataa gagaactgca taggtagtag cgaacttatg
     7681 tgaacgacaa cctcaaaaat gataaaagtt aagacttacg gttcttaaat atgttattta
     7741 aaattaaata taaaaccttc taaataattg ttttaaataa ttacatttgt taacattatt
     7801 ataggtgatt aataaattgt tcaccacagc attatataat atattaaata tgataaaatc
     7861 attaaaaagt gtatcaaaaa acaattaaag tgaggtgata gtagtggaaa aagcttataa
     7921 gtttagaatg tatccaaata aaaaacagca agagttaatt aacaaaactt ttggatgttg
     7981 tagatttgtg tataataagt atcttgctaa aagaatagat gtttataaaa aacataaaga
     8041 aacttttact tataaacagt gtagttctga tttgaccaat ttaaaaaaag aattaaagtg
     8101 gcttaaagaa cctgacaaat tttcactaca aaacgcctta agagatttag ataatgcata
     8161 taaaaaattc tttaaagaaa aagcaggatt tcctaaattt aaatcaaaga aaattaatag
     8221 attttcatat aaaactaact ttacaaatgg aaacattatg tattgtggtc aatatataaa
     8281 actacctaaa cttggtatgg ttaagataag ggataaacaa gtacctcaag gtagaatact
     8341 taatgctact atatctaaag aaccaagtgg tagatattat gtatcattat gctgtactga
     8401 tgtagatatt ggagcatttg aaaatactaa taatcatata ggtttagatt taggaattaa
     8461 agaattttgt atctcaagtt gtggagactt tatagaaaat cctaagtatc ttaagaagtc
     8521 attaaacaaa cttgctaaat tacaaagaga attatcgaga aaaacaatcg gtagtttaaa
     8581 tagaaataaa gcaagattaa aagttgcaag atttcaagaa catatagcta atcaaagaaa
     8641 ggattttcta caaaaattat ctactaaatt aataaaagag aatgatatta tttgcataga
     8701 agatttacaa gtaaagaata tgattagaaa tcgtaaacta tctcgcttaa tttctgatgt
     8761 gtcatggtca gagtttattc gtcaattaga atacaaggct aattggcatg gtagacaaat
     8821 tgtaaaggta ggtaaattct ttgcaagttc acaaatatgc aataaatgtg ggtataaaaa
     8881 cgaggaagtg aaggacttaa atataagaga atggatttgt cctagttgta atgaaactca
     8941 cgatagagat ataaatgcaa gtataaatat attaaaagaa ggattaagac taataacaat
     9001 tcaaaataaa taacgtatgt aagaaccgta ggaactacgg ggatagcctg tggagaatta
     9061 gtaagacata ttttagtatg caaaattctg tgaagcagga accctgtgac tttagtcatg
     9121 ggaggttcag tgtagcaata aatatatatc acctggcttt attgatgcac atatgcatat
     9181 tgaatctaca atggtaatgc caattgaatt atcgaaaaaa ttattaaaat caggtacgac
     9241 aactatcata gccgacccac atgagttggt caatgtaaaa ggagttagtg caatagattt
     9301 tttattagaa tcgacaaaag atataccact taatgtatat attatggtgc cttcatccgt
     9361 acctgcaaca agttttgaaa ctaatggagt tggtaaattt agtgcaaaag acatggagag
     9421 ttatgtaaat aatcctagaa ttttaggttt aggagaggta atgtgtttta atgatgttat
     9481 aaattctgaa aatgaaatct tggataaact agagcttttt aaaaataaag ttgtagatgg
     9541 acatgcacct aatataaatg gtaaatcact tcagacatat gtatgtgcag gaatagaaaa
     9601 tgaccatgaa tgtataactt ttgatgaagt atatgaaaaa ttgagagcag gattaaaaat
     9661 tttaataaga gaaggaagcg cagcaaaaaa tttaaagtct atagtaagtg gtatgctaaa
     9721 gcataacttg ccaattgaag aatttatgtt ttgcacagat gataagcact tagatgatat
     9781 agaaaaacaa ggtcacataa gatggaatat aaaatgtgcg attgatttag gtatggaacc
     9841 tgttagggct ataaaagttg caacttataa ttctgcaaaa gcatatgggt taaggaaaat
     9901 tggagcaata ggtgcgggtt ataaggcaga tattgttgta ttgaatgatt tagacaaaat
     9961 ggaagtggat agtgtatata aagatggtaa tttggtaaac gaagagatgt tttctaatta
    10021 caattatgag attaaagata aagaattgct taatacagtc aagtttaaat atattaataa
    10081 agaaaaaata cagttaaaag taagtgaaaa aaattatgta atggagattg taccttatca
    10141 aattttgaca aataaggtat atgaatcttt accatgtgca gatggatatt ttgttccaaa
    10201 taaagagtat tcaaaacttt gtgttgttga gagacataga atgactggaa atgtagcagt
    10261 tgctccacta aaaggatttg gcataaaaaa tggagcaatt gctactactg tggcccatga
    10321 ttctcataat attatagttg caggggataa tgatgatgat attctagtag ctataaatta
    10381 cttaaaggaa atacaaggtg gttatgtaat tgtatcaaat ggaaaagttc ttgcacacct
    10441 aagtcttcaa gtagctggat taattagtac ttttacagct gaagaagtac aagaaattac
    10501 tgataatatg ttggagatag caagaaaaat gggagttcca gagtatgtag acccttttat
    10561 tacactttct tttatggcac ttccagttgt tccacaaata agacttacag atttaggtct
    10621 atttgatgtt gaggaattta aatttatata gataatataa aagtaataat aaatgaggct
    10681 ttctcaaaat agggataagt ctctttttat tgttgaaatg ataaaaatat atttgatttc
    10741 atacaaaaaa gagcatctca tgaactaaaa attttatttt tagatatcct tattttaatt
    10801 aatctatcat ttaaccttgt tttaagcata gatatcttaa taaaactatt ttcagtctat
    10861 tttaacatat aaatacatga aaattatata tttatgaaac tttatacata aaaaataaaa
    10921 tattttattt atatatagat ttatagggta ataatataag catagtataa aattattaat
    10981 aaaaacaaaa ggagagatat ttatgttgag tataggaaca aaagcaccag aatttacact
    11041 agaagataaa gatggaaata aggtaagtat gtcagatttt aaaggcaaaa aagtagtagt
    11101 atatttttat cctaaagata atacaccagg atgtacacgt caagcttgtg cttttagaaa
    11161 tgcatatgat ggatttaaaa aggaagatat acaagttatt gggataagta aagatagtat
    11221 aaaatcacat caaaaatttg cagaaaagca tgaacttcct tttattttac tttctgaccc
    11281 tgacttagtt gctataaaag cttttgatgt atggaaagag aaaaaaatgt atggaaaaac
    11341 tgcattggga gttgtacgtg cgacatatat tatagatgaa aatggaatta ttgaaaaagt
    11401 ctttgaaaaa gcaaaaccag acacaaatgc acaagagatt cttgaatatt tggaaaaaca
    11461 ggagtaataa gaatttatga ttataatgtt atcaccagct aaaaatatga agaatataga
    11521 agtatttgat agagatttga gtttgccttg ctttatagat aacacaaaag aaatagtgga
    11581 aaatataaag acatttggta tagaagattt taaaaataaa atgaaaatta atgaaaaatt
    11641 agctgtatta aataaaaata gatttgaaag cattaaattt gatagattag gaaaccctgc
    11701 aatcttaact tatgatggaa tacaatataa aaatatagaa gcagaaaatt ttacaaggaa
    11761 agatgaagag tttgcaaata gctgtattcg aatcataagt ggattgtatg gtgttgtaaa
    11821 accatatgat agtatatatg aatatagatt agaaatgcag acaaaattac gagtaggtga
    11881 gtttaagaat ttatacgaat attggggaaa tcgtatatat aaagaactaa tcaaagaaaa
    11941 aacagctatc gttaatcttt catcaaatga atacagcaaa tccatagaaa aatttattaa
    12001 agatagtgat acatatatta catgtacttt taaagtaaat aaaaatggca tattaaaagt
    12061 ggaatctaca caagcaaaga aagctagagg tatgatgaca aaatacattg taaaaaatag
    12121 gataagagat attgaagaac tgaaaaagtt taatttagaa ggttataagt ataaagaaaa
    12181 cttatctaac aatagtgaat acatatttgt aaaagagtag aagttttgtt aaatattaaa
    12241 tggcattgtt aaatttaggt aaaatatact tagtgtgtat tgaattgaac ttgattttaa
    12301 aatataatta ttcttaaatt aaaaaaagaa aggagtggtc ttccaaaatg gaaaatcaca
    12361 aacagatgcc ttggcattca agagcaacgc atgaaatttt agaaagtcta gattcatcag
    12421 aggaggggct caccgatacc gaggcggaaa aacggcttaa agagaatggc ttaaatgaac
    12481 ttagaaaaaa accacctaga acaactttac aaatgctttg gagtcaaatt acagatgcta
    12541 tggttatgat actaatagga gcagcaatat tatctttgat atttggcgaa tttacagaag
    12601 cttttgtaat actaataatt gtaacagtaa atgcagttat tggaattgtt caagagaaaa
    12661 aagcagaatc atcattagaa gcattaaaaa atatgaactc acctacagct cgtgttatgc
    12721 gtgatggtga agaaaatgtt attccagcaa gtaatcttgt agtaggagat attgtttttt
    12781 tagaagatgg agctatggtt ccagcagacc ttcgtttaat agaaacatca agtttaaaaa
    12841 ttcaagaggc ttctttaaca ggagaatctg ttccatcaga aaaagatgca gatacaatct
    12901 tacctaaaga atgtgtatta ggagaccgta gcaatatggc gtatacatct tcaatagtca
    12961 catatggtag aggtgtaggt gttgtagtag caactggtat ggatacagaa gtgggtaaca
    13021 tagcacatct tttagattca caagatgatt ttgatactcc tataaaacgt aaacttaata
    13081 ctgttggaaa gacattaagt gtcataggta ttattgtatg cgttgttata tttgcaatag
    13141 gtgcatttta tcagagacct ctcttgccaa tgtttatgat tgcaatttcc cttgccatat
    13201 caattattcc agagggatta cctgcaacag ctactattgt aatggcttta ggcgtacagc
    13261 gtatggctaa aagaaatgct ttaattagaa aattacctgc tgttgaaaca ttaggaggag
    13321 ctacagtaat atgttgtgat aaaactggta cgttaacctt aaataaaatg actgttacac
    13381 atattgctgt caatggagat tttgaatctg gaaaagtgac atcaatagat actgcatctg
    13441 aaaaacatcc attagtgtat aaagagattg tttatgctgg tgcactttgt aatgatgcat
    13501 gtattaatcc agataaaaca gatgaaatat taggtgaccc aacagagggc gcattaattt
    13561 ttatggccaa aaaatttgga atagatcaag aagctcttga agaagaatat ccacgtgtat
    13621 ttgaacagcc ttttgattca gatagaaaat gtatgacaac tgttcataat atagatgaaa
    13681 agattattgc ttatactaaa ggagcagtag acgaaattct tgacctatgt actaaaatat
    13741 taacttcaaa aggtgaaaga aacattaccg aagtggataa aaagaatata cacgaattat
    13801 gtcttaacat gtctaaagat gcattgcgtg tacttggttt tgctaagcgt gagatttcat
    13861 ctataccaaa agaagatagt gaaaatatag aatatgattt aacatttata ggtatggttg
    13921 gtatgattga cccaccaaga actgaggtaa ttgatgcagt aagtacttgt aaagaagcag
    13981 gtataagaac cataatgata acaggtgacc ataaagttac tgccacaaca atcgctcatg
    14041 agttaggaat atggagtgaa gaaaatactg tgatatctgg tgatgaactt gataatttga
    14101 gtgatgatga actagatgaa gctgttaaaa atactacagt atttgctcgt gtatccccat
    14161 ttgataaact tagaattata caatctctaa aaagaattgg agaagtacca gcaatgactg
    14221 gggatggtgt aaatgactct cctgccttaa agtctgctga tattggtata gccatgggta
    14281 taagtggtac agatgttgca aaagattctt ctgacatgat tttaatggac gatagtttta
    14341 caacaattgc ttatgcaata aaagaaggta gacgtgtata tagaaacata caaaaagtta
    14401 tacaattttt gcttgttggt aatatagcag agatacttac gttgtttgta gcaatattat
    14461 taaattggga tactccgctt cttgctgttc atattctatg ggtaaacctt gcaactgcta
    14521 cgcttccagc ccttgctctt ggagttgacc caccaagcag aaatataatg aagcaccagc
    14581 cagtgaaatc aaccactttg tttgaaaaaa atcttataca aagagttgtt acccaaggta
    14641 tatttgtggc aataatgact attttagcat ataaaattgg tcttaatata gaaaatcatt
    14701 ctgtaggtca aacaatggca ttttgtgtat tagcattttc acagatgtta cgagcattta
    14761 gtcagcgttc aaatacagat tcaatgttta atagaaataa tggtaaaaat ccatttcttt
    14821 taatctcatt tttaacttct gcatttttaa tagcagtaat actatttgca cctgttttta
    14881 gaaatgcttt taacttaaca gttttaaata acttagagtg gcttataacg attgttttag
    14941 ccataatgtc tgtaattcaa gtagaggttg ggaaaattat taaaagaaaa aaattaaaaa
    15001 aatagttgac aaaattagga aaaatatata agatataaaa catagaaaaa ttaaataaat
    15061 cttatccaga gaggtggagg gactggccct atgaaacccg gcaaccagta tatttatata
    15121 cagtggtgct aaatcctgcg gcgaaagctg agagatgagt ataattaagc ttatacagag
    15181 cctgagttat atactcaggc tttatttatt ttaactctca tctggtaaat agtttaattt
    15241 tttttactaa aatctaaggg ggatacaaga atgtataata aagaaacaat atgtgtgcaa
    15301 ggaaattata aaccaggtaa tggagaacca agagtactac ctttatatca aagtacaact
    15361 tttaaatata gcagtataga ccaacttgct gaattatttg atttaaaagt tgatggacat
    15421 atatattcaa gaataagcaa tcctactatt caagcttttg aagaaaaaat aagtttacta
    15481 gagggtggag tatcttctgt agctgtatca tcagggcaat ctgcaaatat gttggcagtt
    15541 ttaaatatat gtaaatcagg agatagtata ctttgttctt caaaagtata tggaggaaca
    15601 ttcaatttac taggacctag tcttaaaaaa tttggtatag atttaatatc gtttgactta
    15661 gattcaagtg aagatgagat agtagaactt gcaaaggaaa atactaaggt tgtgtttgca
    15721 gaaacacttg caaatccaac tcttgaagtc atagattttg aaaaaatagc aaatgtagct
    15781 aagagaatta atgttccatt tattgttgat aattcattag catctccagt gctttgtaac
    15841 cctttaaagt atggagcaaa tatagttact cattctacca caaaatattt agatgggcat
    15901 gcttcaagtg ttggaggaat tatagtggat ggtggaaact ttaactggga taatggaaaa
    15961 tttccagaat tagttgagcc agacccaaca tatcatggta taagctatac tcaaaaattt
    16021 ggaaatgccg catatgcaac taaagcaaga gttcagttgc ttagagacta tggaaattgt
    16081 ttaagcccat tcaatgcgta tcttactaat ttaaatgttg aaacactaca tcttagaatg
    16141 gagagacata gtgaaaatgc acttaaaata gctagatttt tagaaaaaca tgaaaatgta
    16201 gattggatta attacccagg acttgaagat aacaagtatt atgagaatgc caaaaagtat
    16261 ttatcaagag gatgtagtgg tgttttatca tttggagtaa gaggtgggtt agaaaatgcc
    16321 aaaaaatttg tggaaaaatt acagatagca tctttggtta cacatgtttc agatgtaaga
    16381 acttgtgtta tacatccagc ttcaactact catagacaat taacagagga acaattaatt
    16441 gcatctggag tattgccttc actaataaga ttatctgttg gaatagaaaa tgtagaggat
    16501 ttaatagctg atttaaatca agctttaaat ttctaatatt ctaagagaaa gagaagtgaa
    16561 aagatatggc tttaatacta ccaaagggat tacctgttat taataaatta ttagatgaag
    16621 gaatagatgt aatttataaa gaagatttta aaaaggaatt aaagtatgaa gaaaacatag
    16681 acactaagat agctatacta aatctaatgc ctataaagat agatacagaa ttagatttac
    16741 taagaaggat agataaaaca ggattcaacg tgtctgtgga atttattaaa atttcaacaa
    16801 gagaaagtaa aagaagttgt aatgaatatg ttaaagaatt ttataaaaca tttgatgaag
    16861 ccaaaggaga gtattttgat ggctttataa taacaggagc tccagttgaa caaatggagt
    16921 ttgaagaagt tgattattgg aatgaactag aagaaattat ggattattct aagagaaaaa
    16981 ctaaatctac attatatata tgttgggcag ctcaagcaag tctttacaaa tactacaatg
    17041 taaaaaaact tccactaagt caaaaatgtt ttggagtatt taagcataag gtagataaag
    17101 atagtaaaat agtagatgga tttgaaaatg aattttttgc tccacattca agacatacta
    17161 ctgtaaatat tgaagctttg aaagagaata aagagttgag cattgtgagt cattctaaag
    17221 aagctggtcc ctatataatt acaaattcaa gagatgtgtt cgtaatgggt catagcgagt
    17281 atgacaaata cactcttgat aaagaatata agagggatat aaataaagga gataaaatat
    17341 ctatacctca aaattactat attaatgatg acccttcaga agagccaact gtaaaatgga
    17401 aaaaacattc agaattgttg tttagaaatt ggataaaaaa ctatttgata caataaattt
    17461 taagagtata taaatgttaa ctaaaaaaat atagaaattt ataggtataa agttaatctt
    17521 aatgatactt tataccttat ttttttaaaa tttgacctta taatagtagt tttatatttt
    17581 aagaaaatca ctttaggtgt atgtagatag aggtggcttt aattttatat atggtgcgat
    17641 aatgggagac taaaccttta tagtatcaat aaatttgtat tttaactttt aaaagagatt
    17701 aaaaagggag aaatcagtgt aaaatatata tagatttttt attaaatttt attaaaaata
    17761 tcctgtataa aattaaatat caataagata acaaaatttc aattgaagtt acaatattgt
    17821 aattgacaat aaatacttaa tactataata tttaatacat taaacattat agtgttaagt
    17881 atttattaca gtatgcactt cagcctaaat ttgaaataaa gtattttcgt agggagcgtt
    17941 tgtatggatg agatgagttt taagaaagag ctactagagt tgactagaga tataaatatg
    18001 aaatttacaa ctttattaag tgatttttat cagccacttg gtattacagc agtgcaagct
    18061 ttgattttat ctgagttatg tgaacatgga gagaaaaaaa taagcgattt agggaagaac
    18121 ttaaacatga caaatagtaa tgtatcagta atctgtcaaa ggcttgaaaa aaatggcttt
    18181 ttaaatcgca taagagatat agaagaccaa cgaattgtaa aagtaaaagt tacaaataaa
    18241 tcattggata tacaagaaca tatttcaagt agtatttttg atagttattt tgaaaatatg
    18301 accagtgaaa aattgcaaga tatggaagat ataatagaag gtttagagaa attaaataaa
    18361 cttttaactt acattgattg caaataacat aaaaataatt taaaggagaa taaaattatg
    18421 gatgaacata aaccccaaaa aaggtcagca ctctattatt atacagttat aataatagta
    18481 gtaatgtttt taaatgcttt aatatttcca agtttaatgg ataaaaaaat ggttgagatt
    18541 gactatggaa agtttctcac aatgttggat acaggaaagg ttaaatctgt tgagatagaa
    18601 aataatagaa tagcaataaa tccatctagt agcaaggata agaatatata cataactggt
    18661 agaatggatg acccagaatt ggtagataga ctaaaaaatg caaaagtaga atttactaag
    18721 gttattccta aggaaaattc acctctttta agtattttac ttacatggat aatacctata
    18781 ggtattctta tgctatttgg taacttaatg atgaagtcta tgcaaaagcg aatgggtggt
    18841 aatgctatgc aatttggaaa aagcaacgcc aaaatgtatg tatctgctca aagtggaaaa
    18901 agatttaatg atgtagctgg tcaagatgag gcaaaagaag ctttaactga aatagtagat
    18961 ttcttacaca atccagaaaa gtataaaaag ataggagcac aaatgcctaa aggagcactt
    19021 ttagtaggac ctccaggtac aggaaaaaca ctccttgcaa aagctgtagc aggtgaagca
    19081 aatgtaccat ttttctcaat atctggttct gagtttgttg agatgtttgt aggaatggga
    19141 gcttctagga ttcgtgattt atttaaacaa gcaaaagaaa aagctccatg tatagtgttt
    19201 atagatgaga ttgatactat tggtaaaaag cgtgataatg gaagtggatt aggtggaaat
    19261 gatgaacgtg aacaaacttt aaatcagttg ttaagtgaga tggatggttt tgatggtgga
    19321 attggagtag taatacttgc tgctacaaat agacctgaca gtcttgacaa agcattactt
    19381 cgtccaggaa gatttgatag acgtatacca gttgaacttc cagaccttgg aggacgtgaa
    19441 gctatattaa aggtacatgc tcaaaatgta aatgtaagct cagatataga ttataatgca
    19501 attgcaaggt ctacatcagg agcatcaggt gcagaacttg caaatattgt aaacgaagca
    19561 gcacttttag cagttaagtc tggcagagat atagtagaac aacaggattt tgatgaatct
    19621 gttgaaactg taattgctgg gtatcaacgt aaaggagctg taatttcaga taaggaaaag
    19681 aaaattatag catatcatga aataggtcat gcacttgttg ctgctatgca aaaacattct
    19741 gcacctgtcc ataagattac tataatacct agaacttcag gagcattggg atatactatg
    19801 caagttgctg aagatgagag tgtacttatg tctaaagaag aagcaattga taaaataact
    19861 acttttacag gtggtcgtgc tgctgaagag gttatcttta acacttgtac atcaggagca
    19921 tcaaatgata tagaacaagc tactaaaatt gcaagagcta tggttactcg ccttggtatg
    19981 agtgaagagt ttgatatggt cgctcttgaa actgtaagca atcaatattt aggaggagat
    20041 acttctcttg cttgttcttc cgacactgct gctaaaatag atgaagaggt atttaaaata
    20101 gtaaaggctt gtcacaaaaa tgcaattgaa atcttagaaa ataatattga taaattgcat
    20161 gaacttgcta attatttatt agaaaaagaa actattacag gtgatgagtt tatggaaata
    20221 cttaacttaa actaaaatga ttatacacaa gataaaaatg cctattgtta ggcattttta
    20281 ttttacaact aaatttttta aattcatatt taaatcttta cataatctta atcattaaaa
    20341 atacatctta actacaattt tatatcggaa ttgatattat aatagtaatg aatggtgtaa
    20401 ggaggttttt taatggatag acctaatcct gatattctat tagaaaaaat taaaaatgaa
    20461 gaagaaaaac taagtagagg tcaattaaaa atattctttg gttatgcagc tggagtagga
    20521 aaaacctact ctatgttgga atctgcacaa aatttaaaaa aagtgggtgt agatgtagta
    20581 gttggatata ttgaaccaca cactagacca gaaacattgg ctttacttga tggcttggaa
    20641 attcttcctg taaaggagat agaatataaa tctataaaac taaaagaatt tgatttagat
    20701 gctgctttag caagaaaacc agaagttata ttagtagatg aatttgccca ttctaatgtt
    20761 gctgggttaa ggcatacaaa aagatggcaa gatatagaag aattgctgtt agcaggcata
    20821 aatgtctaca ctactgtaaa tgtacaacat ttagaaagtc taaatgacat tgttgagata
    20881 attacaaacg tatcagttaa agagactata ccagataagt ttttggatac aaatacacag
    20941 ttagaactta tagatgttga acctgatgta cttcttgaaa ggttcaatga aggtaagata
    21001 tataagaaag aacaagctac tagagcaaaa aataattttt ttataaaaga taacctggtt
    21061 gcacttagag aaatcgcact tagaaaaact gcagagagag ttaataaaga agttcaaatg
    21121 accagactat caaagggtga tgtaactgta atacctacat cagatacact tcttgcatgt
    21181 ataagtccat ctccatcttc agcaaaagtt attagaactg catccagaat atcagacagt
    21241 acatttgcaa aatggatagc tttatatgtt gaaactccaa acacagcaag acttccattt
    21301 gatgaacaaa aacaactaca aaataaccta aaattagcta agaaattagg tggagaaata
    21361 attgttttac atggtgaaaa tataatagag caaatactta gaattgcaaa gctgagaaat
    21421 gtaacaaaaa ttgtaatagg tagaaatcat tcaagtaata aaagatttag taaaagattt
    21481 aaaaaagata tagttgacaa attaattgat gaagttgatt atatagatat acatataatt
    21541 ccatataaat ttgcctcaga tgttaaatac aaacctaaaa aggataaatc atcaataaaa
    21601 tctaaattta aaattagcaa agttgatttt ataaaactta tatttattac tttgatgata
    21661 agtattttag catatgcagt acaaagcatg ggatttataa gagaaaatat attgttaata
    21721 tatatgttgg gtgtagtttt agtttctatg tggactaaag ggtatagtac aggtataata
    21781 agttctgtat ttaatatagt tcttttaaat tattttttca cagcaccatt atatacatta
    21841 tctatagcag attcaaacta tatagtaaca ctggttgtat tctcaatagt aggtataata
    21901 actagcactc ttacgtctaa gatacagcat gaagcagaaa ctgctgctaa aagagaagaa
    21961 aatacaaaaa tgatatatca aataattaag ggatttttaa gattatcaaa taaagatgat
    22021 atagtaaaca aaggtataga acttttgtct cttagcttaa gtagagatgt aatttgttat
    22081 ttaaatgtag ataagcataa taaatcaaag ctttacaaaa aaaatactaa taataaggat
    22141 aaaaatgatt tggatagtga agatgaaaaa gctgttgcaa gttgggtttt atccaattca
    22201 acagtagcag gaaatgacac ggatacactt cctggttcaa agggatacta tataccaatt
    22261 gtaggaatga atttaacttt aggagtagtg ggaatatcat gtattgattc aaaattggat
    22321 acagaagata taagcttaat tgaaacaata attgctcaaa tggcaatagc gcttgataga
    22381 gaaatcttgt cagaagctaa agaaaatacc aatcttgaga ttgaaaggga aagattaaga
    22441 agtaatcttt taagagcagt ttcgcatgac cttagaactc cactagcagg aatatctggt
    22501 gcagtaagta caattataaa aaacaagggt actataggtc aagatataat agatgaatta
    22561 cttagtggtg tatatgaaga tactcagtgg ttaataagac ttgttgaaaa tttacttagt
    22621 atgaccaaga tagatgaagg gaaattggaa gttaaaaaac attctgaact ggttgaggaa
    22681 attatatcag aagcgttaca aaaaataaaa aaaagaattg aaaatgcact tatagatatt
    22741 aatataccag aacagatatt atttgttcct atggatgcaa aattaataga acaagtttta
    22801 attaatttaa tagataattc tttgaaatat tcaaaggaag attgtaagat agagataaat
    22861 gtatatgaga aagatgatta tgtttggttt gaagtatctg ataatggtcc tggtatatca
    22921 aaggaactta aaaagcatat ctttgacaga ttttttactg gagaagaagg agctaaagat
    22981 tccagaaagg gtgttggact tggattatca atttgtaaat ctataattca agctcataaa
    23041 ggtgaaatta tggtagaaaa caataaagat aagggttcaa cttttaagtt tagtttgcct
    23101 aaggaaaatg aatagtttta aattttaaaa ttagttttgg aggaagaaat gagtaaaaaa
    23161 cttatattgt tagtagagga tgataagaca ataagaaaat ttatatctac tgcactttta
    23221 acacaggatt atgatgtcaa ggaggctata actggaaaag aaggtatatc tatagctgtc
    23281 tcatattctc cagatgttgt tcttttagat ttaggcttag aagatatgga tggaatagag
    23341 gtaataaaag ctatcagaca attttcaaat ataccaataa ttgtagtttc agctagagag
    23401 caagatagag acaaggtaga agtatttgat gcaggggcag atgattactt aacaaaacca
    23461 ttctccatag tagagttatt agcaagagta agagtagcat ttagacattc acaagtagaa
    23521 gctcaacaaa aagatgatgt taaatctacg tttgaagtag ataaattatt gattgatttt
    23581 gataaaagaa aagttattgt tgatgatgtt gaagtccatt tgacacctat tgaatataat
    23641 attctttcat tacttgcaaa gtatcatgga aaagtgctta ctcataattt tataataaaa
    23701 gaaatatggg gtagtgttat agggaatgag actaagtctt taagggtctt tatggcaacg
    23761 ttaagacgaa aaatagaaaa acaacctgca aacccaagat atataattac agaagttggt
    23821 gtaggataca gattaaatga tgaatagttg taatatatta attattagat tatgtgctta
    23881 ttaatatata tta
//
LOCUS       DAEPIN010000059        22537 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.059, whole genome shotgun sequence.
ACCESSION   DAEPIN010000059 DAEPIN010000000
VERSION     DAEPIN010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22537
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.059"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            147..1694
                     /locus_tag="KR250_002690"
     CDS             147..1694
                     /locus_tag="KR250_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBH0068611.1"
                     /translation="MNKNSMTRIYSLNKIIFIIIGILGIVLLTRHIEVDIKVLILILL
                     TLFSINVFISYLKIKLYEDKINLGLINNSDKFINITKDENFLKHVQNYIISNEKENCV
                     MACFDLCRLKLINDIYGYELGDEVLNNILSNLKNYFGKEAIYGKLKSDVFVLIIKLEN
                     REQKIPYLVNLIKNKIVILSQIDSFKMDINIEASIGIYKFNNNEIDIKKAIDNADMAR
                     LKSKGLKHIEYVEFDNAMEEEIQSIMKIERDLFLAIKNKEFVIHYQPKVDSSTGNIIG
                     SEALIRWLHPSLGMVGPNKFIPIAEKNGLINNIGRWLIQEVFITINKWISEGINVLPV
                     SINLSRVELYKNDLVDFFKLMFNTYNIPKELIELEITETTALRDVKFISERLYEIKSL
                     GMNISLDDFGVGNSNFINLKGIPLDIIKIDRSLILDIVTNTKTKFMVKAIVNLSHDLN
                     VTVICEGVEDMHQVKVLSELGCNFIQGYVFFKPLDEMNYKKLLTDGSYINLKDTLLDK
                     CMTVEEE"
     gene            1697..2095
                     /locus_tag="KR250_002691"
     CDS             1697..2095
                     /locus_tag="KR250_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HBH0068612.1"
                     /translation="MISEDVDYSIRIIRAIFNNGEDKPIDAKMILKSEDIPILTLYRL
                     LRKLCANNILTFNKEDGKTYMLNVSLDTISLRTIVEVIDGKIYINKCTRYVSNCSNCL
                     KGCKVNRELLEIEKEIYKLLSRKSIRQILE"
     gene            2135..3868
                     /locus_tag="KR250_002692"
     CDS             2135..3868
                     /locus_tag="KR250_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HBH0068613.1"
                     /translation="MDDISQDNFLLSKEYENSLDVDTKKASGIYYTPKIIVDYIVKKT
                     LKNHDIIKNPYPRILDISCGCGNFLLEVYDILYDLFEENIYELKKKYDENYWTVDNIH
                     RHILNYCIYGADIDEKAISILKDSLTNKKVVNDLDESDIKINLFCCDSLKKKWRYKFD
                     YIVGNPPYIGHKKLEKKYKKFLLEKYSEVYKDKADLYFCFYKKIIDILKQGGIGSVIT
                     PRYFLESLSGKDLREYIKSNVNVQEIVDFLGANIFKNIGVSSCILTFDKKKTKETYID
                     VFKIKNEDICINKFETLEELLKSSKFEHFNINQRLLSDEWILVNKDDETFYNKIQEKC
                     KYSLEDIAISFQGIITGCDKAFILSKDDVKLNLVDDKFLKCWIKSKNINKYIVDKSEY
                     RLIYSNDIDNENTNKRILDEIIGLYKTKLENRRECKSGIRKWYELQWGREKLFFERKK
                     IMYPYKSNENRFAIDYDNNFSSADVYSFFIKEEYLDKFSYEYLVGILNSSVYDKYFKI
                     TAKKMSKNIYDYYPNKVMKIRIFRDNNYEEIENLSKQIISILLNKSIDKGKVEKLQIK
                     MDNLIMDSLGI"
     gene            4100..5311
                     /locus_tag="KR250_002693"
     CDS             4100..5311
                     /locus_tag="KR250_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HBH0068614.1"
                     /translation="MKGLVLEGGGTKGAYQIGAYKALRELGIEFQGVTGTSIGALNGA
                     YIIQNDIEVMEDIWLNYDYTHFMNIDRETYERFKNVDFTTKNLNAVFELINKARKNEG
                     IDISPLRQLMETTLNEDIIRKSKKDFGLVTVWWDKKINPYPLYIEDIPKGRLIDYLIA
                     SSSLPIFQTNMMDDKLYLDGMFCDNMPIGLLEDKGYEDVVVIRLIDDFIGKMNLNRHQ
                     NINIKTIIPSEYLGGSLNKDRDNVEKNIRLGYLDTMKAYERCTGIKYYFDVEYKYNEE
                     YCFDKFRSMSNETIQYICNLLNIKRDASLRTVMESVIPKLGEVLGLPKDFSYKDLFYC
                     IYEKKLEENNINRIKLYDFNKVIDVVNTNIDSNRNLASNFEVRPKISIKKNKNTKLLT
                     NCIIKDIKNQS"
     gene            5504..5764
                     /locus_tag="KR250_002694"
     CDS             5504..5764
                     /locus_tag="KR250_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HBH0068615.1"
                     /translation="MEKVVSIKNASGLHARPAGMFVKKAAEFKSTVEVVAKDKTVNAK
                     SIMGIMSLGLAQGEELKLVVNGEDEEVALAAMVELVESGFGE"
     gene            6118..7830
                     /gene="ptsP"
                     /locus_tag="KR250_002695"
     CDS             6118..7830
                     /gene="ptsP"
                     /locus_tag="KR250_002695"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HBH0068616.1"
                     /translation="MAYKGIGASPGVALGKALVVEHSELVIEKKSIDNVEAEIAKLED
                     AVAVSKEELVKVKEKASEELGAEEAEIFEAHLLVLEDPELIGSAIDKIKTESVNAEYA
                     LNEIKEMFVSMFESMDNEYMKERAADIKDVTNRILRHILGIKVVDLSALSEEVVLIAH
                     DLTPSDTATMNKKMVLGFLTDIGGRTSHTAIMSRTLEIAAIVGLNDITSKVKDGDFVV
                     FNGDTGEVIVNPDEETINKYTELKAKYEDERKALQLLKGKPSVTLDGKHVELAGNIGT
                     PNDIEGLIKNDAEGVGLYRTEFLYMDRDSFPTEEIQYEAYKAVLEGMDGKPIVIRTLD
                     IGGDKELSYLSMEPEMNPFLGYRAIRLCLDRKDIFKTQLRALYRASVHGRLRIMFPMI
                     SSLEELLQAKEVVKEVLAELDSEGVAYAKDVEIGMMIEVPSAAVISDVLAKHVDFFSI
                     GTNDLIQYTCAVDRMNQKISYLYNQFNPAVLRLIKTVIDNAHKEGKWAGMCGESAGDQ
                     KMIPILLGMGLDEFSMSPISILPARKLITSVKESDMKKLADDVLNMGTAEEIKSYIEK
                     TFNI"
     gene            8153..9703
                     /locus_tag="KR250_002696"
     CDS             8153..9703
                     /locus_tag="KR250_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBH0068617.1"
                     /translation="MRYKKRNIDKSSELSFQSLAKYFITFYIVIILIILSMFCINTIW
                     VMKKDNQDLLSTTSNRIEDRVGETIKLLNSLSKDNYIIDNSVNGIDKSKELSMYSDEF
                     GYMMICFVDKNIIVWDEEGGNISLAGRDYMQKLFATKKYQVTDSFAAGADGKTLNYTI
                     ASPIIKNGNVDGCVFAAIYFDEIKDILKQGKGQQQQNFILFGSENQIMNLTSDETAYG
                     EKYMKLSQKTKYLGTNAKKVEKDMFNNKSGDYWAFENGKLYFMSYKPIKNTNWSLFCK
                     VNFLDIFALNLIPMGAILSVITLVFIILICMEKSFMKKRMEVIDMLLGSVQELEKRIY
                     QTERPSEVDFKEIIQLTSKGLTDGLTGVVTRTVFFDQIYNRVEMEENDKLSVISFVDL
                     DDLKCINDLYGHEIGDMVLKNVGYVLREYEIKYNGLIGRYGGDEFVMLLSGFTSREEV
                     EDLLDEIAIRLHTEVLTDDGIIQTHCSVGAVVCSVKDNKDIDNLLKQADEALYYVKRN
                     GKGNYHLV"
     gene            9729..12431
                     /locus_tag="KR250_002697"
     CDS             9729..12431
                     /locus_tag="KR250_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBH0068618.1"
                     /translation="MKNVSKKFFVCTIIVLITFLCFSKFAIYVFDKQEKKETKVVENM
                     LNVYKNNIILNLTEKFNYSKDLTSVLVSNNYSIDKLKPIADEILKNNSCIYISVVEKY
                     NVAAFFSNSVKKDIINTPIQELNYSYSLANSIKGLVVEGPSKDQFSNGDVFLITNPIY
                     KNGEYWGEITVALDAKKVVDSFNLNKLKESGFEYELWRVHLDNGSKSTIEISTKDFNF
                     SKSHEIKFKLPTVWTLSIVYKNGWNNKNIDFIVFVILLIISILIGVMTFIYIEKKSIS
                     KELNQQSLKNLETGILNREGFLIKLQEHLNRKNNEIVLLYICITNYSKFAPVMSFEKR
                     QDYLTHIVKNLDNYIKSSHFIARISEEGFVLALEENCDKNELLNIEKGISLELLRKIK
                     VENKQVFLTIGYGSSRCVDDRKDAESLLLSAIEDYERRFPAMSPIHDFTKKCEDMLDE
                     KKQIVFGEYPDLEINKLSKVIYKYYKKVQRIMYKDYNIDIGNRRGYMRDVEVLIDYNS
                     ERMLYLFLIDICKFGKFNELFSIEIGDVILKEVSNKLKTIFSNYLYRINGDVFLGISI
                     DKESYLDTLHKVNEVFNSSLSVEDVLFDVKCNIGICQYPYHGENPYDLLEKVQIALEY
                     AKKDVKSNVKVYDKELTEEIYHENNIIKDLKKGLEEQTLEVWYQPIFNVSKNCYIKAE
                     ALIRLRNKEGGYYSAYEVITYAEKHGLIKDVGLYVLKKACDVTTKIKDKNIDFEQMQL
                     NLSAQQIVHKQCANDILSCIENCGLDKKYISVEVTEKVLMETFEIVNVTLDKLKKEGI
                     SIALDEFGSGFSSINYLANLPIENLKIDKELVEQITYSKKQFELLKTIVQITKINGMD
                     IVAEGVETKETLDLVVTSGVNLIQGHYFSEPLSEDKFIELMIEE"
     gene            complement(12937..13602)
                     /locus_tag="KR250_002698"
     CDS             complement(12937..13602)
                     /locus_tag="KR250_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0068619.1"
                     /translation="MKGNIVQYNFADIEEEVYSLDYAIAWNTDEENVNIIPFTNKFCK
                     ESIESFCLGKINNFVEILNEGFVENHHYVHLDKMISVPKKKVNLVYQQDTHGYLLRDD
                     NDNLIPAKITSEQSKSISSKMELFCAGEEKCLINILLKADPSYILDVDSIKDKNILNL
                     GYESIDRYKEYNFDDDKILIFFINKKRYSVIMKKTNNSDNDLVSRNNAIKELFTNKAG
                     NLN"
     gene            13946..15376
                     /locus_tag="KR250_002699"
     CDS             13946..15376
                     /locus_tag="KR250_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HBH0068620.1"
                     /translation="MDIKNKIKSIFLPEKNDYLDDEEFEYDIENNEEDLKEDNKVHLN
                     DLSRVKYDYRELKDIENQTEEMCLLAVKQDGTIIEFVKDKTYKVCMEAVKQTYKSLKY
                     ITNQNEDICIEAVKQNYRALYYINNKTENVLIEAIKNASTYDVMEVFKFVEEQTEDVC
                     LAFIERASKNDVAEILKGIKEQTPAICLEAVKKDGKSLAYVKEQSNSICLEAVKENYS
                     ALSCVKEQTEEICIEAVKQNDFALYYVNEQTEKICMEAVKRSYMALQYVNKQTEEICL
                     EAVRIDGRALQYVKEQTEEICLESVRQNGKVLQYVKKQTENICIEAVRGSFEELEIKE
                     ILSYVKIPTERIFVEAVKQNGKILKYVENQTELICLEAVRENYNALAYVKEQTEKICL
                     EAVNQSYEALKYVKEQTEEVCLKAVKQDYRMLKYVNNQTEKICLEAVKQNYRALEFVD
                     NQTEKVCLEAVKQNRKALQYVKQKQS"
     gene            15744..16322
                     /locus_tag="KR250_002700"
     CDS             15744..16322
                     /locus_tag="KR250_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HBH0068621.1"
                     /translation="MFKRYAEKMTSVLICNNMIDNNESKVYSYGFEILIAFIVNITTM
                     LFIGFLFGKFTYVLFFLMCYCPIRQFSGGYHADNYFRCLLTFIFIILSTILIIENINI
                     DLFKNIIMIIASVSWVGICVLCPIEHRSNPISDREKLVYKKTAIFISTVVLLITILSL
                     SLSIFVDYFTYSAFAMFWIFVMLVLGKLKAKV"
     gene            16352..16498
                     /locus_tag="KR250_002701"
     CDS             16352..16498
                     /locus_tag="KR250_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="HBH0068622.1"
                     /translation="MKKFIVRFMKFASSLALSTAILSANSTCPWIIHQPKVPKEISNL
                     KKTN"
     gene            16803..18182
                     /gene="scfB"
                     /locus_tag="KR250_002702"
     CDS             16803..18182
                     /gene="scfB"
                     /locus_tag="KR250_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioether cross-link-forming SCIFF peptide
                     maturase"
                     /protein_id="HBH0068623.1"
                     /translation="MSMIHKFSMNGYNIVLDVNGGAVHVLDDVAYDLLDFYKEKSKEE
                     ILEILKSKYQEEKINEAYEEILNLEKEGLLYTEDTYQYHPSFVHREPVVKALCLNVAH
                     DCNLKCKYCFAAQGDFGGEKELMSFEVGKAAIDYLIANSGSRKNLEIDFFGGEPLMNF
                     EVVKQLVDYGRSVEKDYNKNIRFTITTNGVLLNDEIIDYINENMHNVVLSLDGRKEVN
                     DNMRPTLNDKGSYDITLPRFKKLVEKRAKDKYYYIRGTFTRDNLDFSKDVMHFADLGF
                     KLTSVEPVVGDESNPYALREEDLPKIFEEYEKFAVEYADRQLQGDGFKFFHFMIDLNQ
                     GPCVIKRITGCGAGNEYLSVTPNGDIYPCHQFVGNEEFKMANIFDEEIVLPENLKNMF
                     REAHVYTKEECKQCWNKFYCSGGCHANAINFNNDISKPYELGCEMQRKRTECSIMIQA
                     KLMLEGATN"
     gene            18211..18708
                     /locus_tag="KR250_002703"
     CDS             18211..18708
                     /locus_tag="KR250_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HBH0068624.1"
                     /translation="MIRDYLEDKPLIDESVFVAKSADVIGNVKIGKDSSIWYNAVVRG
                     DEGPITIGENTNIQDCSIVHGDTETIIGNNVTVGHRSIVHGCKISDNVLIGMGSIILD
                     NAEIGEYTLIGAGTLITSNKKFPPGVLIMGSPGKVVRELTEEDKKYIDESYEWYLEAA
                     QNQKY"
     gene            19006..20670
                     /locus_tag="KR250_002704"
     CDS             19006..20670
                     /locus_tag="KR250_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HBH0068625.1"
                     /translation="MRISYRNLKRYREIGYVLIKYGFSFIVERLNIEGIAYKIPLFDP
                     PEEIKNMTTGERMKRVLEELGPTYIKIGQILSTRKDLLDQDIIDEISKLRDDVEKFDS
                     NIAIDIFKEEVGLSIEEIFLEFKEEPIAAASIGQVYEGVLKTGEEVIVKIQRPNIEKI
                     IKSDLEILRTIANTLKDLKKDFNLDLVQMIEEFQTQLMRELDYTFEAINATKFSRIFK
                     NSDEVYIPKVYSEYNTKKILVMEKVNGTKLSDVEKIRRLGYNTKTIVEIGVRSFFTQV
                     LSHGFFHADPHPGNIFVVAKNKIAYIDFGMIGIIDNKTLNQLNEIALAGVEKNVDKII
                     YLLIEMDALNGEADIKGLRQDLLYLIHYYYDISIEKINVTDILNELFRFFRQYKIVMP
                     AQFVTLAKTVITLEGTSRTLNTDFSFGSMGKEFMKHHYKSKFNPKNVVLSSRQNVEEI
                     LLDIKTIPKQLKAILKNIERNNIKMQIEDVKMTRLENCIIELTSQISLSLVLASIIVG
                     SSLIIASPNIENNIWIKFTAIAGFFISFIIGLCLVIRSIRSKYKKD"
     gene            20683..>22537
                     /gene="recJ"
                     /locus_tag="KR250_002705"
     CDS             20683..>22537
                     /gene="recJ"
                     /locus_tag="KR250_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HBH0068626.1"
                     /translation="MKKWILKYKGKIEESEFSKKLNISPEICQILKNRGICTEKDSEI
                     FMNPSLDYLRDPFLMKDMQKAVDRIKLAVEKNEKIWIYGDYDVDGVSSTSILCLYFKS
                     IGYNVDYYIPNRLEEGYGINEDAIKLISSRGCDLIISVDCGITSVSEVNTANDLGIDV
                     IITDHHECQSEIPSAFAVINPKQEDCNYPFDSLCGCGVAFKMIQALTPKEEFKTSMYN
                     YLEIVTLATICDIVPLIDENRIIVKNGLKSMKEGKNIGLRELIKVCGVESDKIGSSHI
                     GFAIGPRINASGRLGYSYLGVELFTTQSQEEAVEIASILEEKNNERQMIEAKMYHEAE
                     EMLKSNSRYNDDKVLVLAKEGWQHGIIGIVASKLTEKYYKPTILLGIENGEATGSARS
                     IKGFNIFEALIKCKDLMTKFGGHEQAAGLSLDSDNVEILANEINKFADYNLTEDDMIE
                     NVNVEFELQENVINLNLVEELHKLEPFGLNNPNPRFIVRNYILKDLKVIGKNQQHLKL
                     SIEKEKSYECIGFNMSHLKSMYKVGDKVDVLFQLDENNYMGNRKVQFLLKDIRLARPK
                     SASNDKLSLKLMSKIIPKDTQSLYNISVSDFELFDGNTDINIFDYFEKDTLI"
BASE COUNT         9128 a         2071 c         3900 g         7438 t
ORIGIN      
        1 taaaatttta aaaaataaat ttagtataat gtaatgtagt tatttatatg tgattttaac
       61 ttgcattgat ttgctatcaa ttgtgcttta taattagtat atataaagtt taactgtaag
      121 ataatttaaa aaatagggga atttatatga ataaaaatag catgacaaga atttacagtt
      181 taaataaaat aatttttatt ataattggaa ttttaggaat agttttactc acaagacata
      241 ttgaggtaga tattaaagtt ttaattttaa ttttattaac cttgtttagt ataaatgtat
      301 ttatttcata tcttaaaatt aagttgtatg aggataaaat taatttagga ttgataaata
      361 attcagataa gtttataaat attactaaag atgagaactt cctaaaacat gttcaaaatt
      421 atattatcag taatgaaaaa gaaaactgtg taatggcttg ttttgattta tgtagactca
      481 aacttataaa tgatatatat ggttatgagc taggggatga agtgttaaat aatattttaa
      541 gtaacttaaa aaattatttt ggaaaagaag ctatttatgg aaagttaaaa agtgatgttt
      601 ttgtattaat aataaagctt gaaaataggg aacagaagat tccttattta gtaaatctta
      661 ttaagaataa aattgtgata ctaagtcaga ttgacagttt taaaatggat ataaatattg
      721 aggcttctat tggaatttat aaatttaaca ataatgaaat tgatataaaa aaagctatag
      781 ataatgctga tatggcaagg cttaaatcaa aaggactaaa gcacatagag tatgtagaat
      841 ttgataatgc tatggaagaa gaaatacaat caattatgaa aatagagaga gatttattct
      901 tggctattaa aaataaagag tttgtaattc actatcaacc taaagttgat tcaagtacag
      961 gtaacattat aggttcagag gcattgataa gatggttgca cccatcatta ggcatggttg
     1021 gaccaaataa gtttatacct atagctgaaa aaaatggtct gataaacaat attggtagat
     1081 ggctaattca agaagttttt attaccatta acaagtggat ttcagaaggt ataaatgtct
     1141 taccagtctc aataaatctt tctagagtag aactatataa aaatgattta gttgattttt
     1201 ttaaactgat gtttaataca tataatattc caaaagaact tatagaatta gaaattacag
     1261 aaacgacagc actaagagat gtaaaattta taagtgaaag gctttatgag attaaatctt
     1321 taggtatgaa tatatcatta gatgattttg gagtaggtaa ttcaaatttt atcaacctta
     1381 aggggatacc actagatatt ataaaaattg ataggtcact tatattggat atagttacaa
     1441 atacaaaaac aaagtttatg gtgaaagcaa ttgttaacct ttcacatgat ttaaacgtaa
     1501 ctgttatatg tgaaggggta gaggatatgc atcaggtcaa agtcttaagt gaactgggat
     1561 gtaatttcat acaaggttat gtatttttta aacctttgga tgagatgaat tataaaaaac
     1621 ttcttactga cggaagttat ataaatctga aagatactct tttagataaa tgcatgacag
     1681 ttgaggagga gtaaggatga tttcagaaga tgtagattat tcaataagaa tcataagagc
     1741 tatatttaat aatggagaag ataaaccaat agatgctaaa atgatattaa aaagtgaaga
     1801 tataccaata ctaacactat acagactgct tagaaagctt tgtgcaaaca atattcttac
     1861 ttttaacaaa gaagatggca agacatacat gttaaatgtg tcattggata caataagtct
     1921 aagaactatt gtagaagtaa ttgatggaaa aatatatata aataaatgta cgagatatgt
     1981 atctaattgc agtaattgct taaaaggttg taaagtaaat cgtgaattgc tagaaataga
     2041 aaaagaaatt tataaacttt tatcgagaaa atctataaga caaatactag aataaaaaat
     2101 tttcagctat gataataatt ggaggttttc ggttatggat gatatatctc aagataattt
     2161 tctactgtct aaagagtatg agaatagctt agatgtagat actaaaaaag caagtggaat
     2221 atattatact cctaaaataa ttgtagatta tattgtgaaa aaaactttaa aaaatcacga
     2281 tataattaaa aatccatatc cacgaatact tgatatttcc tgtgggtgtg gaaattttct
     2341 tttagaagta tatgatattt tatatgactt atttgaggaa aatatatatg aattgaaaaa
     2401 gaagtatgat gaaaattatt ggactgttga taacatacac cgtcatatat taaactattg
     2461 tatatatggt gctgatattg atgaaaaagc tataagtata ttaaaagata gcttaacaaa
     2521 taaaaaggta gtaaatgatt tagatgaatc tgatataaag attaatctat tttgttgtga
     2581 tagtcttaaa aagaaatggc gatataagtt tgattacata gtgggtaatc caccctatat
     2641 tggtcataaa aaacttgaaa agaaatataa aaaatttctc ctagaaaaat atagtgaagt
     2701 ttataaggat aaagctgact tatatttttg tttttataaa aagattatag atattttaaa
     2761 gcaaggaggg attggcagtg ttataactcc aagatatttt ttagaaagtc tatcaggtaa
     2821 agatttaaga gaatatatta aatcaaatgt caatgttcaa gaaatagtgg attttttagg
     2881 tgcaaatata tttaaaaata taggggtatc tagttgtatt ctaacttttg ataagaaaaa
     2941 aacaaaagag acatatatag atgtatttaa aatcaaaaat gaggatatat gtataaataa
     3001 atttgaaaca ttggaagaac ttctaaagag tagcaaattt gaacatttta atataaatca
     3061 gagattgcta tctgacgaat ggatacttgt aaataaagat gatgaaactt tttataataa
     3121 gatacaagaa aaatgtaaat atagcttgga agatatagca ataagttttc aaggaataat
     3181 tacagggtgt gataaagcgt ttattttaag taaagatgat gtaaagttga accttgtaga
     3241 tgataaattt ttaaaatgtt ggataaaaag taagaatata aataaatata tagtagataa
     3301 aagtgaatat aggctaatat actcaaatga tatagataat gagaatacaa ataaaaggat
     3361 actagatgaa attatagggc tatataaaac aaaattagaa aacagaagag aatgtaaaag
     3421 tggaataagg aaatggtatg aacttcaatg gggtagagaa aagcttttct ttgaaagaaa
     3481 aaagataatg tatccttata aatcaaatga aaatagattt gctatagatt atgataataa
     3541 tttttcaagt gcagacgtat attctttttt tataaaagaa gagtatttag ataaattttc
     3601 atatgaatat ctagttggaa ttttaaattc cagtgtttat gataaatact ttaaaataac
     3661 tgctaaaaaa atgagtaaaa atatatatga ttactatcct aataaggtta tgaagataag
     3721 gatttttagg gataataatt atgaagagat agagaattta tcaaagcaaa taatatcaat
     3781 tttattaaat aagtctatag ataaaggaaa agtagaaaaa ttgcagataa aaatggataa
     3841 cttgataatg gattctttag gtatataagc agttgtaagt attaaaaaat tataatcttt
     3901 taattgttta tttaaattta gaaataaaaa gttagccata taaaaaatat taaaaatata
     3961 tacttttcaa tatttttttg ataaaataag aatgaatttg tgcaatttta tttttctcca
     4021 ataactaaaa atagtttttt gaattaatat ttgcttaaga ataaaaatca gaaacgtttt
     4081 tgatatagta ggtgataata tgaaaggatt agttttagaa ggtggaggaa caaaaggagc
     4141 ctaccaaata ggtgcatata aagctttaag agagcttggt atagagtttc aaggagtaac
     4201 tggcacatca attggtgctt taaatggagc atatataatt caaaatgata ttgaagtaat
     4261 ggaagatatt tggttaaatt atgactatac tcattttatg aatattgata gagaaaccta
     4321 tgagaggttt aaaaatgtag atttcactac taaaaatctc aatgcagtgt ttgagcttat
     4381 aaataaagct agaaaaaatg aaggtataga tataagccca ttaagacaat taatggagac
     4441 aacattaaat gaagacataa taaggaaatc aaaaaaagac tttggacttg ttacggtctg
     4501 gtgggataaa aaaattaatc catatccatt gtatatcgaa gatataccta aaggaagatt
     4561 gattgattat ttaattgcta gttccagttt gcctattttt caaacgaaca tgatggatga
     4621 taagttgtat cttgatggaa tgttttgtga taacatgcca ataggactct tggaagacaa
     4681 aggttatgaa gatgttgttg taattaggtt gattgatgat tttattggga aaatgaatct
     4741 aaatcgacat caaaatataa atataaaaac tataatacca tcagaatatc taggaggttc
     4801 tcttaataaa gatagagata atgtagaaaa aaacatccga ctaggttact tagatactat
     4861 gaaagcatat gaaagatgca ctggaattaa gtattatttt gatgtagagt ataaatataa
     4921 tgaagaatat tgttttgata aatttagaag tatgagcaat gaaacaatac aatatatctg
     4981 taatttattg aatataaaaa gagatgcttc acttagaact gttatggaaa gtgtaatacc
     5041 aaagcttggt gaggtgcttg gattgccaaa ggatttttca tataaagatt tgttttactg
     5101 tatatatgaa aagaaacttg aagaaaataa tataaacagg ataaaattat atgattttaa
     5161 caaggttata gatgttgtaa atacaaatat tgattctaat agaaatttag cttctaattt
     5221 tgaagttaga cctaaaatta gtattaaaaa gaacaaaaac acaaaattat tgacaaattg
     5281 tattataaaa gacataaaaa atcaaagtta aataaaaaac aaaaaaaaat ttgtaattat
     5341 actcgaaaaa agtgtatatt agtagtataa tatttttgta gcactagtgg gaacaaaaaa
     5401 tgaccatagg acaaaaaagt accacttagt accattaaag taaaaaacag gaaaacaatt
     5461 ttaaaaaata taaaatgtaa agtttatagg aggatttaaa aaaatggaaa aagtagttag
     5521 tatcaaaaat gcaagtggat tacatgctag accagcagga atgtttgtta agaaggcagc
     5581 tgaatttaaa tcaacagtag aggttgtagc aaaagacaaa actgtaaatg caaaatcaat
     5641 aatgggaata atgagtttag gtttagctca aggagaagaa ttaaagttag ttgtaaatgg
     5701 agaagatgaa gaagtagctt tagctgcaat ggttgaatta gttgaaagtg gatttggaga
     5761 atagatataa taatttttta aaaatttgat atttagaatc taatattact taaagtaaag
     5821 taacagagaa gtattaaaca tatacagacc aacatttgta gatatttaat ttctttgtta
     5881 ctttcatgtt tatttaatca gaagattaag actattgtta attgggtata aaaatataaa
     5941 tattttagaa aatgaaataa gatattttta atcataaatt cagagtaaaa atacagtaac
     6001 aaatagttta taaaatttta taaaaaatta taagtaaata aaactataaa aatgtaaatt
     6061 atttatataa atttataaag tttatgtgct agatgatatt ttttaggagg tagagttatg
     6121 gcttacaaag gaataggtgc ttctcctggt gttgctttag ggaaagcgtt agtagttgaa
     6181 catagtgaat tagtaattga aaaaaaatct atagataatg ttgaagctga aattgctaaa
     6241 ttagaagatg cagttgcagt ttctaaagaa gaattagtaa aagttaagga aaaggcttct
     6301 gaagaattag gagcagagga agcagaaata tttgaagcac atttattagt tttagaagac
     6361 ccagagttaa ttggttctgc aatagataaa attaaaactg aaagtgtaaa tgcagagtat
     6421 gctttaaatg aaataaaaga gatgtttgtt agcatgtttg agtctatgga caatgagtac
     6481 atgaaagaaa gagcggcaga tataaaagat gttacaaata gaatacttag acatatatta
     6541 ggaattaaag ttgttgattt atctgcttta agcgaagaag ttgtattaat agcacatgat
     6601 ttaactccat cagacacagc aactatgaat aaaaaaatgg ttttaggatt tttaactgat
     6661 ataggtggaa gaacttctca tactgctatc atgtctagaa ctttagaaat agctgcaatc
     6721 gttggattaa atgatataac ttcaaaagtt aaggatggag attttgtggt tttcaatgga
     6781 gacacaggtg aagtaatcgt gaatcctgat gaagagacta taaataaata tactgaatta
     6841 aaagctaagt atgaagatga gagaaaagca ttacagctat taaaaggaaa accatctgta
     6901 actttagacg gaaaacatgt ggaattagct ggtaatatag gaacacctaa cgatatagaa
     6961 ggtctaataa agaatgatgc tgaaggtgtt ggtctttata gaacagaatt tttatacatg
     7021 gatagagatt ctttcccaac agaagaaata caatatgaag catacaaagc tgtattagaa
     7081 ggaatggatg gaaagcctat agttataaga acattggata taggtggaga taaagaatta
     7141 agttatttaa gtatggaacc tgaaatgaat ccattcttag gttacagagc aataagactt
     7201 tgcctagata gaaaagatat atttaagact caattaagag ctttatatag agctagtgtt
     7261 catggaagat taagaatcat gttcccaatg atttcatctt tagaagagtt gttacaagct
     7321 aaagaagttg taaaagaagt tttagcagaa cttgattctg aaggtgtggc ttatgctaaa
     7381 gatgtagaaa ttggtatgat gatagaagtt ccatctgctg ctgtaatatc tgatgtattg
     7441 gctaaacacg tagatttctt ctctatagga actaatgatt taattcaata cacttgtgca
     7501 gttgatagaa tgaatcaaaa gataagctat ctttacaatc aatttaatcc agctgtttta
     7561 agacttataa agactgttat agacaatgca cataaagaag gcaaatgggc aggtatgtgt
     7621 ggagaatctg ctggagacca aaaaatgata ccaatacttt taggtatggg attagacgag
     7681 ttctctatgt ctccaatatc aatacttcct gctagaaagt taataacttc agttaaagag
     7741 tcagatatga aaaaattagc tgatgatgta cttaatatgg gtactgctga agaaataaaa
     7801 agctatatag aaaagacttt taatatataa ttaatgttat atagttacta aaattcattt
     7861 ttctaaataa caaaattata ttttacgaat aaaaaaacta tagtatatca tatatactat
     7921 agtttttttg ttatataaat tacgtatcaa aatttgtaaa atgaatagat ataattacta
     7981 aatatgtgat agtattggct ataataggat ataaaaaata atattgtaaa aaaaagttta
     8041 tatttagtaa tgaaaattta acgttttact tgaaattatg tgttttgtaa tgtattatta
     8101 aggctctaat aggaacattt tcatcaattt ttctatagtg ggggtaggaa ttatgaggta
     8161 caaaaaaaga aatatagata agtcaagtga actttcattt caatctctag caaaatattt
     8221 tataactttc tatatagtaa ttatattaat tattctttct atgttttgta taaatacaat
     8281 atgggttatg aaaaaagata atcaagattt attatctaca acatcaaatc gtattgaaga
     8341 tagggtagga gaaacaatta agctattgaa ctctttgagt aaagataatt acataataga
     8401 taatagtgtg aatggcatag ataaaagtaa agaactttca atgtatagtg atgaatttgg
     8461 gtatatgatg atatgttttg ttgataagaa tataattgtg tgggatgaag aaggtggtaa
     8521 tattagtcta gctggtcgtg attatatgca gaaacttttt gcaacaaaaa aatatcaagt
     8581 tacagatagt tttgcagcag gggctgatgg aaaaacttta aactacacta ttgcatcacc
     8641 tataataaag aatggaaatg tagatggttg tgtatttgca gcaatttact ttgatgagat
     8701 aaaggatata ctcaaacaag gaaaaggtca acagcaacag aatttcatat tatttggaag
     8761 tgaaaatcaa ataatgaatt taacaagtga tgaaactgca tatggagaga agtatatgaa
     8821 gttatcgcag aaaacaaaat atcttggaac caatgcaaaa aaagtagaaa aggatatgtt
     8881 taataataaa tcaggtgatt attgggcttt tgaaaatgga aaattatact ttatgtccta
     8941 taaaccaatt aagaacacaa attggagttt attttgtaaa gttaatttct tagatatttt
     9001 tgcattaaat ttaattccta tgggagctat tctatctgtt ataactttag tatttattat
     9061 tttgatttgt atggagaaaa gcttcatgaa aaaacgaatg gaagttattg atatgctttt
     9121 aggttctgtt caagaattag aaaaacgtat atatcaaaca gaaagaccta gtgaagtaga
     9181 ctttaaagaa attattcagc tcacaagtaa aggtctgact gatggattaa ctggtgttgt
     9241 aacacgtact gtattttttg accaaattta taatcgtgtt gaaatggagg aaaatgataa
     9301 attatctgta atttcatttg ttgatttaga tgatttgaaa tgtattaacg acttatatgg
     9361 acatgaaatt ggggatatgg ttttgaaaaa tgttggatat gtacttcgtg aatatgagat
     9421 aaaatataat ggtttaattg ggcgctatgg tggagatgaa tttgttatgt tgttatctgg
     9481 ttttacatcg agagaagagg tggaggattt attggatgag attgctattc gcttacatac
     9541 tgaagtacta acagatgatg gtatcattca aactcattgt agtgttggag ctgtagtatg
     9601 tagtgtaaaa gacaataaag atatagataa tttattaaaa caggctgatg aagctttata
     9661 ctatgttaaa cgtaatggaa aaggtaatta tcatttagtt taatgtatgt ggaaggagtt
     9721 tatatcatat gaaaaatgta tctaaaaaat tttttgtatg tactattatt gtattaataa
     9781 catttttatg ttttagtaaa tttgctattt atgtttttga caaacaagaa aagaaagaaa
     9841 caaaagtagt agaaaacatg ttaaatgtct acaagaataa cattatttta aatctgactg
     9901 aaaaatttaa ttatagcaaa gacttgacat cagttttagt ttcaaacaat tattctatag
     9961 ataaattaaa acctattgca gatgaaattc ttaaaaacaa ttcttgcata tatatttcag
    10021 ttgtagaaaa atataatgta gctgcatttt tttctaattc tgtcaaaaaa gatattatca
    10081 atactccaat acaggaactg aattattctt attcattagc aaattctatc aaaggattag
    10141 tcgtagaagg tccttctaaa gaccagtttt caaatggaga tgtgttttta attactaatc
    10201 ctatttataa aaatggagag tactggggag aaattacagt agctttagat gccaaaaaag
    10261 ttgtagattc ttttaactta aataaattaa aagaatctgg atttgagtat gaactttggc
    10321 gtgtacacct agataatggt tcaaaatcta caatagaaat ttctactaaa gattttaatt
    10381 tttccaaatc acatgaaatt aagtttaaac ttccgactgt ttggactctt agcattgtgt
    10441 acaaaaatgg atggaataat aagaatatag attttattgt atttgttata ttgttaatta
    10501 tttctatatt aataggtgtg atgacattta tttacattga aaagaagtcg attagtaagg
    10561 aattaaatca acagagtctt aaaaatttag aaactggtat tttgaataga gaaggttttt
    10621 taattaagtt gcaagagcat ctgaatagaa aaaataatga aatagtgttg ctgtatatat
    10681 gtattactaa ttatagtaaa tttgctccag ttatgagctt tgaaaaaaga caagattatc
    10741 ttacacatat agtaaaaaat ctagataatt acataaaaag ttctcatttt attgctcgta
    10801 taagtgagga aggttttgtt ctagctttag aagaaaattg tgataaaaat gaattgctta
    10861 acattgaaaa agggatttcc ttagagcttt tacgaaaaat taaagtagaa aataaacaag
    10921 tatttttaac gattggttat ggctcatcta ggtgtgttga tgatagaaaa gatgcagaat
    10981 ctctgttact ttcagctatt gaagattatg agagaagatt tccagcaatg tctcctattc
    11041 atgattttac taaaaagtgt gaagatatgc ttgatgaaaa gaaacagatt gtatttggtg
    11101 aatatccaga tttagaaata aacaagttgt ctaaagtaat atacaaatat tataaaaaag
    11161 tacagcgtat tatgtataaa gattataata ttgatattgg aaataggcga gggtatatgc
    11221 gtgatgtcga agtattaatt gattataatt ctgagagaat gttatactta tttttaatag
    11281 acatatgcaa gtttggaaaa tttaatgaac tgttttctat tgaaatagga gatgttattt
    11341 taaaagaagt tagtaacaaa ttaaaaacaa ttttttctaa ttatctatat cgtattaatg
    11401 gagatgtatt tttaggcatt tcaatagata aagaatctta cttagatact ttacataagg
    11461 ttaatgaagt atttaatagt tctttaagtg tagaagatgt actttttgat gtaaaatgta
    11521 atattggtat ttgtcaatat ccatatcatg gtgaaaatcc atatgacctt ctggaaaagg
    11581 tacaaattgc attggagtat gcaaaaaagg atgtcaaaag taatgttaaa gtttatgata
    11641 aggaattgac agaagagatt taccatgaaa ataatattat taaggattta aaaaaaggtc
    11701 tggaagaaca aacactagaa gtttggtatc agccaatttt taatgttagt aaaaactgtt
    11761 atataaaagc tgaggcacta attagattga gaaataaaga gggcggttat tattctgcct
    11821 atgaagttat tacttatgca gaaaagcatg gacttattaa ggatgtaggt ctatatgtac
    11881 taaaaaaggc ttgtgatgta actacaaaaa taaaagataa aaatatagac tttgaacaaa
    11941 tgcaattaaa tttatctgct cagcagattg tacataagca gtgtgcaaat gacatattat
    12001 catgtattga gaattgtggt ctagataaaa agtatatttc tgttgaagtt acagaaaaag
    12061 tacttatgga aacttttgaa attgtaaatg ttactcttga taaactaaaa aaagaaggca
    12121 ttagcatagc tttagatgag tttggttctg gatttagtag tataaattat ctggcaaatt
    12181 tacctattga aaatttaaaa atagataagg aattagtaga acaaattacc tattctaaaa
    12241 aacaatttga attattaaaa actatagttc aaattactaa gattaatggc atggatattg
    12301 tagcagaagg tgttgaaaca aaagaaactc tggatttagt tgtaacttca ggtgtgaatt
    12361 tgattcaagg tcattatttt tcagaacctt taagtgaaga taaatttatt gaacttatga
    12421 ttgaagaata aaggttaata aaataaagat ttaaggtatt gaaaaataat taaaagtatt
    12481 taataataga attagggaag aataagttta taagtaaaaa caattgatta gtagttaata
    12541 aaataaatgt gatatccaaa taaatcctaa atctaatttg agttttaata tagatagtct
    12601 aaagtttgat tattaaagat ttaattgtaa gttgttacag ttgatgatat tgattagaga
    12661 ttaaagttta gagtagaaag ttaccttaaa tttaaaaatg caagataacc caatctaaaa
    12721 attataatca ctcgataggt ttaaggattt atttgtttat tataaatagg agggtgtctc
    12781 aaaatgaact ttttagttta tgaggcactc tcttttatat agaattaaat atattttatt
    12841 atttcaacaa tgaaaaagaa gcttatctct attttgagac agcctctttg tatactatta
    12901 ttaatttaat caataacata atttaaaatt ataatactag ttaagatttc ctgctttatt
    12961 agtaaataat tctttaatag cattgtttct ggaaactaaa tcgttatcag agttgtttgt
    13021 ttttttcata atcactgaat aacgtttttt atttataaaa aatattaaaa tcttatcatc
    13081 atcaaaattg tattctttat atctgtcgat agactcataa cctagattta gaatgttttt
    13141 gtcttttata ctatctacat ctaaaatata tgatgggtca gcctttagta atatattaat
    13201 taaacatttt tcttcaccag cacagaataa ctccatctta cttgatatag atttagattg
    13261 ctctgaagtt atttttgcag gaataaggtt gtcattatca tctcttagaa gataaccatg
    13321 tgtgtcttgt tgatatacta aattaacctt tttcttagga acactaatca ttttatctaa
    13381 atgtacataa tgatgatttt caacaaaacc ttcatttaat atttcaacaa agttattaat
    13441 tttacctagg caaaaagatt ctatagattc tttacagaat ttatttgtaa atggtattat
    13501 gtttacgttt tcctcgtctg tattccatgc tatagcataa tccaacgaat atacttcttc
    13561 ctctatatct gcaaaattat attgtactat gtttcctttc aaaattactc gacctccatc
    13621 ttataatata tttaatatac tttataaaac ttatattata tttaaataat atcataaatt
    13681 gaataaatat gatatagtaa ataagaaaaa atttgaaaaa aatcactata aatgagtaat
    13741 aaatataaat agtgtatact aatgggatgg aaaatgtcca ctattaactt tattacccca
    13801 catatataag ataaaattat taatataaaa tataatctat cgacaatgaa tagactttta
    13861 aataaactta atataaaatt aattaaataa gtgcttaaaa acgatttact ttaatgatag
    13921 aatcatatta aaggaggata attttatgga tataaagaat aaaattaaat ctatattttt
    13981 accagaaaaa aatgactatt tagatgatga agaatttgag tatgatatag aaaataatga
    14041 agaagattta aaggaagata acaaagtaca tttaaatgat ttaagcagag taaaatatga
    14101 ttacagagag ttaaaagaca tagaaaacca aacagaagag atgtgtctgc tagcagtaaa
    14161 gcaggatggt actataattg agtttgtaaa ggacaagact tataaagtat gtatggaagc
    14221 agtaaaacag acatataaat ctcttaagta tataacaaat caaaatgagg atatttgtat
    14281 agaagcagta aaacagaact acagagcttt gtactacata aataataaga cagaaaatgt
    14341 actaattgag gctataaaaa atgcatcaac ttatgatgtc atggaagtat ttaaatttgt
    14401 ggaagagcaa acagaagatg tatgtctagc atttatagaa agggcatcta aaaatgatgt
    14461 tgcagagata ttaaaaggaa taaaagagca aactccagcc atatgtttag aagctgtaaa
    14521 aaaagatgga aaatctttag cttatgtaaa ggaacaaagt aatagtatat gtttagaagc
    14581 tgtaaaagaa aactacagtg cactatcttg tgtaaaagag caaactgaag aaatatgtat
    14641 agaagcagta aaacagaatg actttgcatt gtattatgta aatgaacaga cagaaaaaat
    14701 atgtatggaa gcagtaaaaa gaagttatat ggctctacag tatgtaaata agcaaacaga
    14761 agagatttgt ttggaagcag taagaattga tggaagagca ttacagtacg taaaagagca
    14821 aacagaagaa atttgcttgg agtcagttag acaaaatgga aaagtactgc aatatgtaaa
    14881 aaaacagact gaaaatatat gtatagaagc agtaagaggt tcatttgaag aacttgaaat
    14941 aaaagaaata ttaagttatg taaagatacc aacagaaaga atatttgtag aagcagtaaa
    15001 gcaaaatgga aaaattctta aatatgtaga aaatcagaca gaattaatat gtttagaggc
    15061 agtaagagaa aactataatg ctttagctta tgtaaaggaa caaacagaaa agatttgttt
    15121 agaagcagtt aatcaaagtt atgaagcgct taaatatgtg aaagaacaaa cagaagaagt
    15181 ttgtctaaaa gcagtaaaac aagattatag aatgttgaaa tatgtaaata atcaaacaga
    15241 aaaaatatgt ttagaagcag taaagcaaaa ttatagagct ttagaatttg ttgataatca
    15301 aacagaaaaa gtttgtcttg aagcagtaaa acagaataga aaagcactac agtatgtaaa
    15361 acaaaaacaa agttaaaagg ctaaaatata tagaaaacat atgcaaataa ataaaatttg
    15421 atattttgta ataaataaaa tgggtatctc aaaaggaact ttttagtttg tgaggtactc
    15481 tttttatatg aaatgaaata tatttttagc cttttaacaa tggggaaaag gtttatttct
    15541 attttgacat aggcttttta tgcatataaa gcttgataaa attgttttat tataattgtt
    15601 ttgttatata aataataaga agaaaattat ataggtcttt ttttgaccag ttttacattt
    15661 ttaacaacta gttttgtaaa gtatattgaa ttttctcaaa aaagaatgta ctctatatat
    15721 gtaaaatttt tttggaggta aaaatgttta aaagatatgc tgaaaaaatg acatctgttt
    15781 taatttgtaa taatatgata gataataatg aatctaaagt ctattcttat ggatttgaaa
    15841 tattaatagc tttcatagtt aatataacca ccatgctgtt tataggtttt ttatttggta
    15901 aatttacata tgtattattt tttttaatgt gttattgtcc aataagacaa ttttcaggag
    15961 ggtatcatgc agacaattat tttagatgct tattaacctt tatttttata attttatcta
    16021 caatcttaat tatagaaaat ataaatatag acttatttaa aaatattatt atgattattg
    16081 caagtgttag ttgggttgga atttgtgttc tgtgcccaat agaacatagg agtaacccta
    16141 taagtgatag ggaaaaattg gtttataaga aaactgctat atttatatct acagtagtgt
    16201 tattgataac aattttaagt ctttctttga gtatatttgt agattatttc acatatagcg
    16261 catttgccat gttttggatt tttgtgatgt tagtattagg taagttaaaa gctaaagtat
    16321 aagtaaaata tgtatgaggg agagttaagt tatgaaaaag tttattgtaa gatttatgaa
    16381 gtttgctagc tcattggcac ttagcactgc tattttgtca gctaatagta cctgtccttg
    16441 gattattcat cagcctaaag tacccaaaga aatcagcaat ctaaagaaga caaattaaat
    16501 acaagcactt atttatacaa aattatcagg aaaaaagaaa ttattatatt ttaataagat
    16561 taaaacaata taaaatcaga tacaaaattt attttattta ttttatagga gcctaatacc
    16621 tatttggtaa tatattagtg cgccaattta gggggatata gtgtatttaa tgaaatgact
    16681 tacggagagt aagtcatttt tgtattgtat gtattttatg taaacttatg atataatgaa
    16741 gggtaaagtt taattaaaat aataaaaaac tttagtatag atagtaattt aggaggtagg
    16801 gtatgagtat gatacataaa ttttctatga atggatataa tatcgtttta gatgtgaatg
    16861 gtggagctgt tcatgtgtta gatgatgttg catacgattt gttagatttt tataaagaaa
    16921 aaagtaaaga agaaatatta gagatattaa aatcaaaata tcaagaagaa aaaataaatg
    16981 aagcatatga agaaatatta aatttggaaa aagagggtct tttatataca gaagatacat
    17041 atcaatatca tccaagcttt gtacatagag agcctgtagt aaaagcttta tgtttaaatg
    17101 tagctcatga ttgtaactta aaatgtaaat actgttttgc agctcaaggt gattttggag
    17161 gagaaaaaga acttatgagt tttgaagttg gtaaggcagc aattgactat ttgattgcta
    17221 attcaggaag tagaaagaac ctagagatag atttctttgg tggagagcct ttgatgaact
    17281 ttgaggttgt aaaacaactg gttgactatg gaagaagtgt agaaaaagat tataataaaa
    17341 atataagatt tacaataact acaaatggtg tactgctaaa tgatgagatt atagattata
    17401 taaatgaaaa tatgcataat gtagttttaa gtttagatgg aagaaaagaa gttaatgata
    17461 atatgagacc aactttaaat gataaaggaa gttatgatat tacattacca cgatttaaga
    17521 aacttgtgga aaaaagagca aaagataagt attattatat aagaggtact tttacaagag
    17581 ataatttaga tttttcaaaa gatgttatgc attttgcaga tttaggattt aaattaacct
    17641 ctgtagagcc agttgttgga gatgagagta atccatatgc acttagagaa gaagatttac
    17701 ctaagatatt tgaagagtac gaaaaatttg ctgtagagta tgcagataga caattacaag
    17761 gagatggatt taaatttttc cattttatga tagacctaaa tcaaggtcca tgtgtaataa
    17821 agagaataac tggatgtgga gctggaaatg agtatctttc tgtaactcca aatggagata
    17881 tatatccatg tcatcaattt gttggaaatg aagaatttaa aatggcaaat atatttgatg
    17941 aagaaatagt acttccagaa aatttaaaaa atatgtttag agaagctcat gtatatacta
    18001 aagaagagtg taaacaatgt tggaataagt tttactgttc aggtggatgt catgcaaatg
    18061 cgataaactt taataatgac atatcaaaac catatgaatt aggatgtgaa atgcaaagaa
    18121 aacgtacaga gtgctctata atgatacaag ctaagttaat gttagaagga gcaaccaact
    18181 aaatttatat tttaaaggag ttgtttatat atgattagag attatttaga agataaacct
    18241 ttaattgacg aaagtgtatt tgtggcaaaa tcagcagatg taattggaaa tgttaaaata
    18301 ggcaaagatt caagtatatg gtacaatgca gtagttaggg gtgatgaagg accaataact
    18361 attggagaaa acactaatat acaagattgt tctatagtgc atggagatac agaaactata
    18421 attggtaaca atgtaacagt tgggcatagg tctatagttc atggttgtaa aattagtgat
    18481 aatgtgttaa ttggtatggg ttcaataata ttggataatg ctgaaatagg agaatatact
    18541 ctgataggag caggtacact aattacatct aataagaagt ttccaccagg tgttttgata
    18601 atgggttctc ctggtaaggt tgttagagag cttacagaag aagataaaaa atatattgat
    18661 gaatcttatg aatggtattt agaggcagca caaaatcaaa aatattagtt taaagagtgg
    18721 ttgtttcaaa acatatttaa atctgttttg aggcaaccac taatttattt tattagattt
    18781 tgttgatatt ttagttttca aaaagtattt gattaaacca gatataaaat gtcaaaaata
    18841 aaaattaact gagttaaata attttaataa aattggtagt atatgtgata aaattaaata
    18901 ttaaagagga tataggatat tagaatatca attggtgaaa taatgtcata tacgataaat
    18961 taaggtgtaa gtttttgggt atacctatct taggagtgaa aagatttgag aataagttat
    19021 agaaatttaa agcgatatag agaaattgga tatgttttaa taaagtatgg ttttagtttt
    19081 attgtagaaa gactaaatat agaaggaatt gcctataaaa taccactatt tgacccacca
    19141 gaagaaataa aaaatatgac aactggtgag agaatgaaaa gagttttaga agaactagga
    19201 cctacatata taaaaatagg acagatattg agtacaagaa aagacttgtt agaccaggat
    19261 ataattgatg aaatatcaaa actcagagat gatgtagaga aatttgattc aaatatagct
    19321 attgatatat ttaaagaaga agtgggttta agtatagaag aaatattctt agaatttaaa
    19381 gaagaaccaa tagcagcagc ttcaattggt caagtttatg aaggtgtact taaaacggga
    19441 gaagaagtaa tcgtaaaaat tcaaagacca aatatagaga aaataataaa atctgattta
    19501 gagattctta gaactattgc aaatacttta aaagatttaa aaaaggattt taatttagat
    19561 ttagtacaga tgattgaaga atttcaaact caattaatga gggaacttga ttatacattt
    19621 gaagctataa atgcaactaa attctctagg atatttaaaa atagtgatga agtatatata
    19681 cctaaagtat attcagaata taatactaag aaaattttag taatggagaa agttaatggt
    19741 acaaagttaa gtgatgtaga aaaaataagg agactaggtt ataacacaaa aactattgtt
    19801 gaaataggag ttaggtcatt ttttacacag gtattatctc atggattttt tcatgcagac
    19861 ccacatccag gtaatatatt cgtagttgct aaaaataaga tagcatatat agattttgga
    19921 atgattggaa ttattgataa taaaacttta aaccaattaa atgaaattgc attagcaggt
    19981 gtagaaaaga atgttgacaa gataatatat ctattgatag aaatggatgc attaaatgga
    20041 gaggctgata ttaagggact tagacaggat ttactttatc taatacatta ttactatgat
    20101 atttctatag aaaaaataaa tgtaacagat atattaaatg aactatttag gttttttaga
    20161 cagtataaaa tagtcatgcc tgcacaattt gtaactcttg caaagacagt tataacatta
    20221 gaaggaacaa gtagaacttt aaacacagat ttttcttttg gttctatggg aaaagagttt
    20281 atgaaacatc attataagag taaatttaac cctaaaaatg ttgttttaag ttcaagacaa
    20341 aatgtagaag aaatattact ggacataaaa actattccaa agcaattaaa agcaatttta
    20401 aaaaatatag aaagaaataa tataaaaatg caaattgaag atgttaaaat gacgaggtta
    20461 gaaaattgta taatagagct aacatctcaa atatctttga gtttagtgtt ggcatctatt
    20521 atagtaggtt cttctcttat aattgcatct ccaaatatag aaaataatat atggataaaa
    20581 tttactgcaa tagcgggttt ctttatatcc tttataatag gcctttgctt agttataaga
    20641 agtattaggt caaaatacaa aaaagattag ggggaattaa atttgaaaaa atggatattg
    20701 aagtataaag gtaagataga ggaaagtgaa tttagcaaaa aacttaatat ttccccggaa
    20761 atctgccaaa ttttaaaaaa tagagggata tgtactgaaa aagattcaga aatatttatg
    20821 aatccatctt tagattattt aagagaccct tttttaatga aagatatgca aaaagctgtt
    20881 gatagaataa agttagcagt tgagaagaac gaaaaaattt ggatttacgg agattatgat
    20941 gttgatggtg tatcatctac atctatcttg tgcttatatt ttaagagtat aggttacaat
    21001 gttgattact atattcctaa tagattagaa gaagggtatg gtataaatga agatgctatt
    21061 aagcttataa gctctagggg atgtgacttg attataagtg ttgactgtgg aattacatca
    21121 gtgtctgagg tcaatacagc gaatgatttg ggcatagatg ttataattac agaccatcat
    21181 gaatgtcaaa gtgaaattcc atcagccttt gcagtaataa atccaaaaca agaagattgt
    21241 aattatccat ttgattcact atgtggatgt ggtgtagcat ttaaaatgat tcaagccttg
    21301 actccaaaag aagaatttaa aactagtatg tataattatt tggaaatagt aacactagca
    21361 actatttgtg atattgttcc acttatagat gaaaatagaa taattgtaaa aaatggttta
    21421 aaatcaatga aagaaggcaa aaatattgga ttaagggagc ttataaaagt ttgtggtgta
    21481 gaaagtgata agataggttc ttctcatata ggttttgcaa taggtcctag gataaatgct
    21541 tcaggtcgat tgggatattc ttacttagga gtagagctat ttactactca atcacaagaa
    21601 gaggcagtag aaatagctag tatattggaa gaaaaaaata atgaaagaca gatgatagaa
    21661 gctaagatgt atcatgaagc tgaagaaatg ctaaagtcta atagcagata caatgatgac
    21721 aaggtgttgg ttttagctaa agagggttgg caacatggaa taataggaat tgtagcatca
    21781 aagttaactg aaaaatacta caaaccaaca attcttctag gtatagaaaa tggggaggct
    21841 actggttcag cgaggtcaat aaaaggattt aatatatttg aagctctcat aaagtgcaaa
    21901 gacttgatga ctaagtttgg tggtcatgaa caagcagcag ggttatcctt ggattcagac
    21961 aatgtagaaa ttttagcaaa tgaaataaat aaatttgcag attataattt aactgaagat
    22021 gatatgatag agaatgtaaa tgttgagttt gaattacaag aaaatgtaat aaatttaaat
    22081 cttgtagaag aattacataa gttagaaccc tttggattaa ataatcctaa tcctagattt
    22141 atagttagaa attatatttt aaaagatttg aaagttatag gtaagaatca acaacatctg
    22201 aaattaagca tagaaaaaga gaaaagctat gaatgtatag gatttaatat gagtcattta
    22261 aaatcaatgt ataaagtagg agacaaggta gatgtactat tccaattaga tgaaaataac
    22321 tatatgggaa ataggaaagt acaattttta cttaaagata tacgattggc tcgacctaaa
    22381 agtgcaagta atgacaaact ttcattgaaa ttaatgtcaa aaataatacc aaaagatact
    22441 caaagcctgt ataatatatc tgtgtctgat tttgaattat ttgatggaaa tacagatata
    22501 aatatatttg attattttga aaaagataca ctaataa
//
LOCUS       DAEPIN010000060        22313 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.060, whole genome shotgun sequence.
ACCESSION   DAEPIN010000060 DAEPIN010000000
VERSION     DAEPIN010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22313
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.060"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            <1..369
                     /gene="tuf"
                     /locus_tag="KR250_002706"
     CDS             <1..369
                     /gene="tuf"
                     /locus_tag="KR250_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887863.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBH0068627.1"
                     /translation="DNIGALLRGVQRNEIERGQVLAKTGSVKAHTKFTAEVYVLKKEE
                     GGRHTPFFDGYRPQFYFRTTDVTGACKLPEGIEMVMPGDNVTMEVDLINSIVVEEGLR
                     FSIREGGRTVASGVVATIIE"
     gene            741..1052
                     /gene="rpsJ"
                     /locus_tag="KR250_002707"
     CDS             741..1052
                     /gene="rpsJ"
                     /locus_tag="KR250_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HBH0068628.1"
                     /translation="MAKNEKIRIRLKSYDHKLLDFSAGKIVETAKKAGSQVSGPVPLP
                     TEKQVVTILRAVHKYKDSREQFEIRTHKRLIDIANPTPKTVDSLMRLDLPAGVDIEIK
                     L"
     gene            1144..1773
                     /gene="rplC"
                     /locus_tag="KR250_002708"
     CDS             1144..1773
                     /gene="rplC"
                     /locus_tag="KR250_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HBH0068629.1"
                     /translation="MKGILGKKVGMTQIFTDKGVVIPVTAVEAGPMVVTQIKTVDKDG
                     YNAIQIGFEDAKEKALNKPKKGHLAAANVLKKHLKEFRVDSVEGYTVGQEIKADVFEA
                     GAKIDVTGISKGKGFQGPIKRHGQSRGPETHGSRYHRRPGSMGACSYPGRVFKNKKLA
                     GHMGSVKVTVQNLEVVKVDADKNLILVKGAIPGAKGSVVTIKEAIKVSK"
     gene            1803..2426
                     /gene="rplD"
                     /locus_tag="KR250_002709"
     CDS             1803..2426
                     /gene="rplD"
                     /locus_tag="KR250_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001127258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HBH0068630.1"
                     /translation="MPKLNVLNVSGQNVGEIELSDSIFGVEVNGHVLYEVVKNQLANK
                     RQGTQSAKTRAEVRGGGRKPWKQKGTGRARQGSTRSVQWVGGGVAFAPKPRSYKYTLP
                     KKVRRLAMKSALSSKVQNSEVIVLDALNMDAPKTKEFAQILNNINAAKKALVVIADKN
                     DNVIKSARNIEGVQTALVNTMNVYDILKYDSFIITTDAVKKVEEVYA"
     gene            2426..2716
                     /gene="rplW"
                     /locus_tag="KR250_002710"
     CDS             2426..2716
                     /gene="rplW"
                     /locus_tag="KR250_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HBH0068631.1"
                     /translation="MTNPHDVIIRPVVTEHSMAEMGEKKYTFVVAKDANKTEIKKAVE
                     KVFGVSVDKVNTLNYDGKVKRMGRTQGRTSSFKKAVVKLTADSKEIEFFQGM"
     gene            2746..3576
                     /gene="rplB"
                     /locus_tag="KR250_002711"
     CDS             2746..3576
                     /gene="rplB"
                     /locus_tag="KR250_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HBH0068632.1"
                     /translation="MAIKKFRPTSPALRQMTVLVSDEITCNQPEKSLLVNLKKNAGRN
                     VHGRITVRHRGGGQKRKYRIIDFKRDKDGIPAKVATIEYDPNRTANIALLNYADGEKR
                     YILAPVGINVGDTILSGLGADIKPGNCLALKDMPVGTIIHNIELKPGKGAQLVRSAGV
                     SAQLMAKEGKNALLRLPSGEMRLVSINCKATIGQVGNIEHGNVVIGKAGRKRHMGIRP
                     TVRGSVMNPNDHPHGGGEGRSPIGRPSPVTPWGKPALGYKTRKKNKASNKLIVSRRTK
                     "
     gene            3611..3892
                     /gene="rpsS"
                     /locus_tag="KR250_002712"
     CDS             3611..3892
                     /gene="rpsS"
                     /locus_tag="KR250_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HBH0068633.1"
                     /translation="MSRSTKKGPFVHARLLKKIEAMNASGNKEVIKTWSRSSTVFPQM
                     VENTIAVHDGRKHVPVYITEDMVGHKLGEFVPTRTFKGHKDDEKSNKRK"
     gene            3924..4259
                     /gene="rplV"
                     /locus_tag="KR250_002713"
     CDS             3924..4259
                     /gene="rplV"
                     /locus_tag="KR250_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HBH0068634.1"
                     /translation="MEAKATAKYVRVSPRKAGQICDLVRGKNVDEALAILKFTPRGAA
                     SIIAKVVKSAKANAENNHEMDTEKLYIASIVANQGPTMKRFMPRAMGRATTIRKRTSH
                     IEVVVKEKK"
     gene            4282..5097
                     /gene="rpsC"
                     /locus_tag="KR250_002714"
     CDS             4282..5097
                     /gene="rpsC"
                     /locus_tag="KR250_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HBH0068635.1"
                     /translation="MGQKVNPHGLRVGVIKDWDSRWFATDKKEFGNLLLEDHNIRKFL
                     KKRLYSAGVAKIEIERSANKIKMDLHVAKPGVVIGRAGAGIEALKAELEKMTKKTIIV
                     NIVEVRSTDKNAQLVAENIALAIERRVAFRRAMKQAIQRAMKSGAKGIKVSASGRLGG
                     AEMARTEGYSEGNVPLQTLRADIDYGFAEADTTYGKIGIKVWICNGEVLPTRDGVNPR
                     EESRKSDRRDNKRDNRRNDRRGNDRRGNDNRGNYRGQRPQGGSRPQRTENKGN"
     gene            5133..5564
                     /gene="rplP"
                     /locus_tag="KR250_002715"
     CDS             5133..5564
                     /gene="rplP"
                     /locus_tag="KR250_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HBH0068636.1"
                     /translation="MLMPKRVKRRRVHRGSMAGQAHKGNKVTYGEFGLVALEASWITS
                     NQIEAARIAMTRYIKRGGKVWIKIFPHKPVTRKPAETRMGAGKGSPEYWVAVVKPGRV
                     MFELAGVSEDKAREAMRLAAHKLPIKCKFVKKEDLEVKGGE"
     gene            5566..5769
                     /gene="rpmC"
                     /locus_tag="KR250_002716"
     CDS             5566..5769
                     /gene="rpmC"
                     /locus_tag="KR250_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005810148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HBH0068637.1"
                     /translation="MKAKELRDLTSEELMNKLNDFKSELFSLRFQLATGQLENTARIK
                     FVKKDIAKVKTVLAERKLYETRA"
     gene            5793..6047
                     /gene="rpsQ"
                     /locus_tag="KR250_002717"
     CDS             5793..6047
                     /gene="rpsQ"
                     /locus_tag="KR250_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HBH0068638.1"
                     /translation="MERGRRKVRIGRVVSDKMDKTIVVAVEEFVRHPLYNKRVKRTKK
                     FKAHDEKNICNIGDRVKIMETRPLSKDKRFRLVEVVEKVK"
     gene            6073..6441
                     /gene="rplN"
                     /locus_tag="KR250_002718"
     CDS             6073..6441
                     /gene="rplN"
                     /locus_tag="KR250_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007885948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HBH0068639.1"
                     /translation="MIQQESRLRVADNSGAKELLCIRVLGGSKRRYGNIGDVIVATVK
                     SATPGGVVKKGKVVKAVIVRSKQGVRRNDGSYISFDENAAVIIKDDKTPVGTRIFGPV
                     ARELRDNEFMKIVSLAPEVL"
     gene            6465..6770
                     /gene="rplX"
                     /locus_tag="KR250_002719"
     CDS             6465..6770
                     /gene="rplX"
                     /locus_tag="KR250_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HBH0068640.1"
                     /translation="MRVKKGDTVVVIAGKDKGKKGSVLKVYPKTSKVLVEGVNVITKH
                     QKPSAMNQQGGIINKEAPIHISNVMPFDPETGKGVRVRYEVKDGNKVRVSAKSGKEL"
     gene            6800..7342
                     /gene="rplE"
                     /locus_tag="KR250_002720"
     CDS             6800..7342
                     /gene="rplE"
                     /locus_tag="KR250_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HBH0068641.1"
                     /translation="MASRLQEKYMKEVAPALMEKFGYKNVMEIPKLNKIVINMGIGDA
                     RENPKGLEKGVEELEMISGQKPVITKARKSVANFKLREGMPIGTKVTLRADKMFYFMD
                     KLVSVSLPRVRDFRGVNPNAFDGRGNYALGVKEQLIFPEIEYDKIDKVRGMDIIFVTT
                     AKTDEEARELLKLLGMPFSK"
     gene            7360..7545
                     /locus_tag="KR250_002721"
     CDS             7360..7545
                     /locus_tag="KR250_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HBH0068642.1"
                     /translation="MARKAMVVKQQRKQKYATREYTRCTICGRPHSVLKKFGICRICF
                     RELAYKGQIPGVRKASW"
     gene            7578..7976
                     /gene="rpsH"
                     /locus_tag="KR250_002722"
     CDS             7578..7976
                     /gene="rpsH"
                     /locus_tag="KR250_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HBH0068643.1"
                     /translation="MTMTDPIADMLTRIRNANVVKHETVDVPASNMKKELARILLEEG
                     FIRGYDVIEDGKQGIIRIQLKYGQEGERVITGLKKISKPGMRVYAANHEIPKVLNGLG
                     ISVISTSKGILTDKQARKENVGGEVICYVW"
     gene            8007..8549
                     /gene="rplF"
                     /locus_tag="KR250_002723"
     CDS             8007..8549
                     /gene="rplF"
                     /locus_tag="KR250_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HBH0068644.1"
                     /translation="MSRIGVKPIIIPAGVEVTIAEGNLVTVKGPKGTLTKQLSAELNI
                     KKEENTIMVERPTDNKKHRSLHGLTRTLLDNMVVGVNTGFEKKLELKGVGYRAQKQGK
                     KLVMNLGFSHPVEMEDPEGITVEAPNQTELIVKGIDKQLVGNYAAKIRAWRKPEPYKG
                     KGIKYVDEVIRRKEGKTGKK"
     gene            8585..8953
                     /gene="rplR"
                     /locus_tag="KR250_002724"
     CDS             8585..8953
                     /gene="rplR"
                     /locus_tag="KR250_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HBH0068645.1"
                     /translation="MLKKADKNANRLQRHKRVRRKISGTSQRPRLCVFRSANNIYAQI
                     IDDTKRVTLVAASSLEAEVKSAVNHTGNKEAAKKVGELVAKKAVEKGITEVVFDRGGY
                     LYHGRIQELAEGAREAGLKF"
     gene            8974..9483
                     /gene="rpsE"
                     /locus_tag="KR250_002725"
     CDS             8974..9483
                     /gene="rpsE"
                     /locus_tag="KR250_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011109776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HBH0068646.1"
                     /translation="MLRRKPIDAGQLDLQEKVVEVRRVTKVVKGGRNFRFAALVVVGD
                     ENGHVGIGAGKAMEVPDAIKKAVEDAKKNLIVVPIVGTTIPHEVRGHFGAGNILIMPA
                     VEGTGVIAGGPARAVLELAGLKDVRAKSLGSNNPRNMVNATIEGLNSLKTVEDIAKLR
                     GKKVEELLG"
     gene            9498..9683
                     /gene="rpmD"
                     /locus_tag="KR250_002726"
     CDS             9498..9683
                     /gene="rpmD"
                     /locus_tag="KR250_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HBH0068647.1"
                     /translation="MAKLQIKLVRSVIGTTPNQKKNVEALGLRKREQVVVKEDNAQTR
                     GMINKVSHLLEVTEIAE"
     gene            9716..10159
                     /gene="rplO"
                     /locus_tag="KR250_002727"
     CDS             9716..10159
                     /gene="rplO"
                     /locus_tag="KR250_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000766080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HBH0068648.1"
                     /translation="MKLHELKPAEGAVRAKRRLGRGTATGQGKTAGRGQKGQWSRSGG
                     GVRVGFEGGQMPLARRLPKRGFNNIFKKVYTEVNVEVLNRFENGTEITAELLKSTKTI
                     SKIGKDGIKILGEGNLEKALTVKAAKFTASAQEKIEKAGGKAELV"
     gene            10203..11471
                     /gene="secY"
                     /locus_tag="KR250_002728"
     CDS             10203..11471
                     /gene="secY"
                     /locus_tag="KR250_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HBH0068649.1"
                     /translation="MLSKLKQAWKIKAVRKKVMYTLMMIVIFRIGTTIPVPGIDTSII
                     QKMVGGNSLLSLYNMFTGGAFSNFSLFALGISPYITASIIIQLLTVGFESLAELQKSG
                     EEGKKKINKYTKYTALALAVVQALGITLGIVRSALISNSVFFITTVVITLVSASMLVM
                     WIGDKITEKGIGNGSSVIIFAGIISRIPTDVIKISQQVKSGEVAPWVIVILAVVILLT
                     VTGVTFIQEATRKIPVQYAKRVVGRKMYGGQSSHIPMKVNQSGVMPIIFASSLLAFPQ
                     TIAMFMGPNAQAFVQKYLSMATEQGFWTYRSIEILLIIFFSYFYTTVSFNTEDISKNM
                     KNNGGFIPGIRPGEPTMDYLNRILTRLTLAGATFLAIIAMVPALTTHYMKVNMSLAGT
                     SLLIVVGVALELKRQLESNLVMRSYQGFLK"
     gene            11488..12138
                     /locus_tag="KR250_002729"
     CDS             11488..12138
                     /locus_tag="KR250_002729"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HBH0068650.1"
                     /translation="MRIILLGPPGAGKGTQAAGIVEKYNIPHISTGDIFRKNIKEGTE
                     LGKKAKEYMDQGLLVPDELTVGLVTDRISQEDCKNGFMLDGFPRNVAQGEHLDIFLKN
                     AGISLDKVVNIEVDKSILVSRAVGRRICKSCGATYHVEFNPPKVEGVCDVCQGELYQR
                     ADDNEETVSKRIQVYLDETKPLVDYYSKQGIIADIKGDQAIDKVFEDIVAALGSGK"
     gene            12139..12885
                     /gene="map"
                     /locus_tag="KR250_002730"
     CDS             12139..12885
                     /gene="map"
                     /locus_tag="KR250_002730"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HBH0068651.1"
                     /translation="MIILKSKKEIELLREAGKIVADTHEVLRKAISPGISTLELDKIA
                     EENIRKYNAEPSFKGYGGFPGSICASINREVVHGIPGETILQEGDIVSLDIGAYYKGY
                     HGDSAKTHGVGMISEEDRKLIEVTRESFYEGIKFAKLGYRLSDISHAVQTHVEKHGFS
                     VVRDLVGHGVGANLHEDPQVPNYGLPGKGPRLREGMVIAIEPMVNAGRYHVKTLSDGW
                     TTVTIDGKKSAHYEHTIAITEHEPLILTKL"
     gene            12907..13176
                     /locus_tag="KR250_002731"
     CDS             12907..13176
                     /locus_tag="KR250_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KOW domain-containing protein"
                     /protein_id="HBH0068652.1"
                     /translation="MLSDNLSIGQVVKVSLGRDKGNLFFVVKIINNEYVLIADGKKRK
                     LDKPKLKKVKHLKKYNFINDEVRKRVVSGQEITDSFLRAELTKLN"
     gene            13214..13432
                     /gene="infA"
                     /locus_tag="KR250_002732"
     CDS             13214..13432
                     /gene="infA"
                     /locus_tag="KR250_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HBH0068653.1"
                     /translation="MAKKDVIELEGTVSEALPNAMFKVKLENGHEILCHISGKLRMNF
                     IRILEGDKVNVELSPYDLTRGRITWRKK"
     gene            13468..13581
                     /gene="rpmJ"
                     /locus_tag="KR250_002733"
     CDS             13468..13581
                     /gene="rpmJ"
                     /locus_tag="KR250_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HBH0068654.1"
                     /translation="MKVRPSVKPICEKCKVIKRKGKVMVICENPKHKQKQG"
     gene            13734..14105
                     /gene="rpsM"
                     /locus_tag="KR250_002734"
     CDS             13734..14105
                     /gene="rpsM"
                     /locus_tag="KR250_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005810116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HBH0068655.1"
                     /translation="MARIAGVDLPREKRAEIGLTYIYGIGKATANEILAKAEINPDTR
                     IKDLSEDQVNELRKVIDDDFLVEGDLRREIALNIKRLRDIKCYRGIRHAKGLPLRGQR
                     TKTNARTRKGPRKTVSRKKKK"
     gene            14141..14539
                     /gene="rpsK"
                     /locus_tag="KR250_002735"
     CDS             14141..14539
                     /gene="rpsK"
                     /locus_tag="KR250_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HBH0068656.1"
                     /translation="MAKPKKKVTRIRRRERKNIERGHAHIQSTFNNTIITLTDVHGNA
                     ISWASSGQLGFKGSRKSTPFASQMAAETAAKAAMEHGLKSVEVFVKGPGSGREAAIRA
                     LQATGLEVTMIKDVTPIPHNGCRPPKRRRV"
     gene            14571..15194
                     /gene="rpsD"
                     /locus_tag="KR250_002736"
     CDS             14571..15194
                     /gene="rpsD"
                     /locus_tag="KR250_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HBH0068657.1"
                     /translation="MARYTGASCRQCRREGMKLFLKGDRCYTDKCAIVKRNYAPGQHG
                     QGRKKVSNYGLQLREKQKVKRIYGVLETQFRNLYERAENMPGKAGENLLSLLERRLDN
                     VVYRMGLASSRKEARQLVTHGHFTLNGNKVDIPSLIVKVGDVIEVKEKSRSSAKFKNL
                     VEVNSRIAPKWLEANVEGMTAKVVGVPTREDIDLEIAEHLIIELYSK"
     gene            15272..16219
                     /locus_tag="KR250_002737"
     CDS             15272..16219
                     /locus_tag="KR250_002737"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HBH0068658.1"
                     /translation="MIEIEKPKVDIVELSEDYRYGKFVIEPLERGYGITIGNALRRIL
                     LSSLPGVAVNAIKIDGVLHEFSTIPGVKEDVTEIILTLKELSATIDGEGSRTLKIEAQ
                     GPCSITGADIICPPDVEILSKDLAIATLDDNAKLNMEIFVDKGRGYVSAEENKTENVP
                     IGVLPVDSIYTPVEKVSYHVENTRVGQKTDYDKLVLEVWTNGSINPQEGISLAAKVLV
                     EHLNLFIDLTEHVSSVEIMVEKEEDQKEKVLEMTIEELDLSVRSYNCLKRAGINTVEE
                     LANKSEDDMMKVRNLGKKSLEEVIQKLEELGLGLKPSEE"
     gene            16240..16581
                     /gene="rplQ"
                     /locus_tag="KR250_002738"
     CDS             16240..16581
                     /gene="rplQ"
                     /locus_tag="KR250_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003357477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HBH0068659.1"
                     /translation="MAKYRKLGRETAHRNLMLRNLVTCLLRSGRIETTVTRAKETRRM
                     AEKMITLAKRGDLHARRQVLAYVMDETVVNNLFTDLAPKYAERNGGYTRIIKIGPRKG
                     DAAEMAFIELV"
     gene            16701..17534
                     /locus_tag="KR250_002739"
     CDS             16701..17534
                     /locus_tag="KR250_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HBH0068660.1"
                     /translation="MDNIVKVNNISFEYITDEAKLKAIDNLSLDVKKGEFVAIIGHNG
                     SGKSTLSKNLNAILMPTEGNILIDDMDTKEEERLWDIRQTAGMVFQNPDNQIVATIVE
                     EDVAFGPENLGIEPKEIRRIVEESLKSVGMYDLRDRQPHLLSGGQKQRVAIAGIIAMR
                     PKCIIFDEATAMLDPSGRKEVMKTIKRLNKEENITVIHITHFMEEAVEADRVVVMEKG
                     KKILEGTPREVFSKIKMLKEIGLDVPCMTELSSLLIEEGINISSDILTVDEMVMELCQ
                     L"
     gene            17522..18388
                     /locus_tag="KR250_002740"
     CDS             17522..18388
                     /locus_tag="KR250_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HBH0068661.1"
                     /translation="MSIIVKNLTHIYNEGMPFASKALDDVSFEIKDRDFVGLIGHTGS
                     GKSTLIQHLNGLLKPSSGEIFINDFNITDKNLNLTEIRKRVGVVFQYPEYQLFEETID
                     KDIAFGPSNLGLEESEIHNRVKASMEAVGLDYEGFKDKSPFELSGGQKRRVAIAGVIA
                     MNPEVLILDEPTAGLDPGGRDEIFNLIKDLHEKKNMTIILSSHSMDDMAKLAKTLIVM
                     NHGSVEFMGTPREVFKSNASKLKDIGLDIPQVLELALKLREKGFDISEDILTLEEAKQ
                     EILKVVRGRGLC"
     gene            18382..19185
                     /locus_tag="KR250_002741"
     CDS             18382..19185
                     /locus_tag="KR250_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HBH0068662.1"
                     /translation="MLKDITIGQYYPTSSAIHKLDPRIKLVATIVFMVSIFVVNKFWP
                     YIVVLLCLLAMIKLANIPVKYIVKGVKPLKWIILFTFLINIFFLPGDEIWSFGFLAIT
                     KQGLRQAIFMAIRLIFLVVGTSLLTLTTSPIELTDGIERLLNPFKKIGLPVHELAMMM
                     TIALRFIPTLLDETDKIMKAQMSRGADFESKNLINRAKNLVPLLVPLFVSAFRRADEL
                     AMAMEARCYRGGHNRTKMRESVISRRDYMACVFQVVYLGAIIATRFIAI"
     gene            19217..19948
                     /gene="truA"
                     /locus_tag="KR250_002742"
     CDS             19217..19948
                     /gene="truA"
                     /locus_tag="KR250_002742"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HBH0068663.1"
                     /translation="MRNIKIKIQYNGKNYCGWQKQPDSLGIQGTIERAIYDITKEETS
                     LIGSGRTDSGVHAIGQIANFKINSGISIESIPMALNAKLPKDISVIEACEVNDDFHSR
                     YSAKGKTYKYLVYNSKFRNPILSEISYQVKYELDFDKMCSEAKSLLGTHDFKGFMSSG
                     SSVKDTVRTIYDIDISKKDDLITFEISGNGFLYNMVRIIVGTLVDMGRGRINEPFLDI
                     IQSKTRSRCGHTAPAQGLFLKKVHY"
     gene            20068..20499
                     /gene="rplM"
                     /locus_tag="KR250_002743"
     CDS             20068..20499
                     /gene="rplM"
                     /locus_tag="KR250_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HBH0068664.1"
                     /translation="MKSYIAKPADVQRKWYLVDAEGKTLGRLATEIATVLRGKHKPTF
                     TPHVDGGDFVVVVNAEKIVLSGKKLDQKYYRYHTGYVGGLKEISYRDMMDKKPEEVIS
                     HAVSGMLPKNKLRSRMMTRLRVFAGAEHTHAAQNPEVLNFK"
     gene            20528..20920
                     /gene="rpsI"
                     /locus_tag="KR250_002744"
     CDS             20528..20920
                     /gene="rpsI"
                     /locus_tag="KR250_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HBH0068665.1"
                     /translation="MANVQYYGTGRRKSSVARVRLVAGEGNILVNGRALENYFNYETL
                     IRDVKQPLVLTGNENKYDVIVKVEGGGFTGQAGAIRHGISRALLKADLDLRPALKKEG
                     FLTRDARMKERKKYGLKAARRAPQFSKR"
     gene            21379..22131
                     /gene="cwlD"
                     /locus_tag="KR250_002745"
     CDS             21379..22131
                     /gene="cwlD"
                     /locus_tag="KR250_002745"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase CwlD"
                     /protein_id="HBH0068666.1"
                     /translation="MEYYSVFISIHIQGDVVRKYIKHIIFSFAMICLVVVSIFEIKNI
                     SEDVIKYMPVTNKTIILDAGHGGIDPGALNKDKSTSEKDINLAITLKLRELIESSGGL
                     VILTREDDSSLYKEENNKTTRQKYNENLKNRKEIISNSNANMFVSIHLNAFEQSKYYG
                     AQTFYPKDKQDSKELSKCIQEELKRVVDKTNNREVKPRDDIYLLKENNIPSVLIECGF
                     LSNEKECKLLTDETYQEKIAWAIYIGIQKYLS"
BASE COUNT         8969 a         2609 c         4585 g         6150 t
ORIGIN      
        1 gataatatag gagcattatt aagaggagta caaagaaacg agatagaaag aggacaagta
       61 ctagcaaaga ctggatcagt aaaggcacac acaaagttta cagcagaagt atatgtactt
      121 aaaaaagaag aaggtggaag acatacacca ttctttgatg gatatagacc acaattctat
      181 ttcagaacaa cagacgtaac aggagcttgt aagttaccag aaggaataga gatggtaatg
      241 cctggagata acgtaacaat ggaagtagac ttaataaact caatagttgt agaagaggga
      301 ttaagattct caataagaga aggtggaaga acagtagctt caggagttgt tgctacaata
      361 atagagtaat cctttactaa actaatggat ttaatataaa aaaagagggg ggtaactcct
      421 ctcttttttg ttttttgttg acaagtagcg ctaaagcata tatactaaac tagtgccttg
      481 agacagatta acaatcaagt ttaaggttat gatgttgtaa tagaaaataa aattgaaaaa
      541 aacagaaaaa aaacttgaaa aaatgtaaca gatgatataa aatataatag tgtgcttgag
      601 agattaagaa aataataatt aaatcctagt gaaatacgaa ctgatatcga tttaaaagtt
      661 acaacacacc cggagcagtg tatcggtatt ggttgtacgc aacttgaaaa ttaaacacgt
      721 gataaaggag ggaaaacaaa atggctaaga atgaaaaaat aagaataaga ttaaaatcat
      781 atgatcacaa attattagat ttttcagcag gtaaaatagt tgaaactgct aaaaaggctg
      841 gatcacaagt ttcaggacct gtgcctctac caacagaaaa acaagttgta actatattaa
      901 gagctgtaca taagtacaaa gactctagag aacaatttga aataagaact cataaaagac
      961 taatcgacat agctaatcca acacctaaga cagttgactc attaatgaga ttagacttac
     1021 cagcaggtgt agatatagaa ataaaattat aagaacccct aagatattat cctatagatg
     1081 attatcttag aatgattact ctaaaagatg taatccgctg taagaattat aggaggtgct
     1141 aatatgaaag gaatattagg caaaaaagtt ggtatgactc aaatattcac tgataaaggt
     1201 gtagttatac ctgtaacagc tgttgaagca gggcctatgg tcgttactca aataaaaaca
     1261 gttgataaag atggatacaa tgctatacaa ataggttttg aagatgctaa agaaaaagct
     1321 ttaaacaaac ctaaaaaagg acatttagct gctgctaatg ttttaaagaa acatttaaaa
     1381 gaatttagag tagattctgt agaaggatac acagtaggac aagaaataaa agctgatgta
     1441 tttgaagcag gtgcaaaaat agatgttact ggaataagta aaggtaaggg attccaaggt
     1501 ccaataaaaa gacatggaca aagtagaggt cctgaaactc acggttctag ataccacaga
     1561 agaccaggtt caatgggagc ttgttcttac ccaggtagag tatttaaaaa caaaaaatta
     1621 gctggacata tgggaagcgt aaaggtaaca gttcaaaact tagaggttgt taaagtagat
     1681 gctgacaaga accttatatt agttaaagga gctataccag gagctaaagg ttcagtagta
     1741 actataaaag aagctataaa ggtttctaaa taatgactaa cctagagaaa ggaggaataa
     1801 caatgccaaa attaaatgta ctaaatgtta gtggacaaaa cgttggagaa atagaattat
     1861 cagattctat atttggcgta gaagttaatg gacatgtttt atacgaagtt gtaaaaaatc
     1921 aattagcaaa taagagacaa ggaactcaat ctgctaaaac tagagcagaa gttagaggtg
     1981 gcggaagaaa accttggaaa caaaaaggaa caggtagagc aagacaaggt tctacaagat
     2041 ctgtacaatg ggtaggtgga ggagttgctt ttgcacctaa accaagaagc tacaaatata
     2101 cattacctaa gaaggtaaga agattagcta tgaagagtgc tttatcttca aaagttcaaa
     2161 atagtgaagt tatagtatta gatgcattaa acatggatgc tcctaagact aaagaatttg
     2221 ctcaaatatt aaacaatata aatgctgcta aaaaagcttt agtagttata gctgacaaaa
     2281 acgataacgt aataaaatca gctagaaata tagaaggtgt tcaaactgca ttagttaata
     2341 ctatgaatgt atatgatata ttaaaatacg attcatttat aataactaca gatgctgtta
     2401 aaaaagtgga ggaggtgtac gcataatgac taatccacat gatgtaataa taagaccagt
     2461 tgtaactgag cacagtatgg ctgaaatggg tgaaaaaaaa tacacttttg ttgtggctaa
     2521 agatgcgaac aaaactgaaa taaaaaaagc agtagaaaaa gtattcggag ttagtgttga
     2581 taaagtaaat actttaaact acgatggaaa agttaaaaga atgggtagaa ctcaaggaag
     2641 aacatcaagc tttaagaaag cagtagttaa attaactgct gatagtaaag aaatagaatt
     2701 cttccaagga atgtagtatt ataacaaacg aaggagggaa aacagatggc tataaaaaag
     2761 tttagaccaa cttctcctgc cttaagacaa atgacagttt tagtttctga tgaaataaca
     2821 tgtaatcaac cagaaaaatc tcttttagtt aacttaaaga aaaatgctgg tagaaacgta
     2881 catggtagaa taactgttcg tcatagaggt ggaggacaaa aaagaaaata tagaataata
     2941 gattttaaaa gagataaaga tggtatacct gctaaggttg ctactataga atatgatcca
     3001 aacagaactg caaatatcgc attattaaac tatgctgatg gagaaaaaag atatatatta
     3061 gctccagttg gaattaatgt tggagatact atattatcag gattaggtgc tgatataaaa
     3121 ccaggaaact gtttagcatt aaaagatatg ccagttggta ctatcattca taatatagaa
     3181 ttaaagccag gaaaaggagc tcaattagtt agatctgctg gagtttctgc acaattaatg
     3241 gctaaagaag gaaaaaatgc tttattaaga ttaccatcag gtgagatgag attagtaagc
     3301 ataaactgta aagctactat aggtcaagtt ggaaatatag agcatggtaa cgtagttatc
     3361 ggtaaagcag gtagaaaaag acatatggga ataagaccta ctgtaagagg ttctgtaatg
     3421 aaccctaatg accatccaca cggtggaggg gaaggtagat ctccaatagg tagaccatct
     3481 cctgttactc catggggtaa accagctctt ggatacaaaa ctagaaagaa aaataaagct
     3541 tcaaataaac taatagtatc aagaagaact aagtagtaaa tttatttttg ctcggaaagg
     3601 aggaatataa atgtcaagat caactaaaaa agggcctttt gtccatgcaa gacttttaaa
     3661 gaagatagaa gctatgaatg ctagtggaaa taaagaagtt ataaaaactt ggtcaagatc
     3721 ttcaactgta tttccacaaa tggtagaaaa cactatagct gttcatgatg gaagaaaaca
     3781 tgttccagta tatataacag aagatatggt tggacataaa ttaggtgagt tcgttcctac
     3841 tagaactttc aaaggacata aggatgacga aaaatctaat aagagaaaat aattaaatta
     3901 tgactaagaa aggaggaata acaatggaag caaaagctac tgctaaatac gtacgtgtat
     3961 caccaagaaa agcaggccaa atatgtgacc ttgttagagg aaagaatgtt gatgaagcat
     4021 tagcaatatt aaagtttact ccaagaggag cagcttcaat aatagctaag gttgtaaaat
     4081 cagctaaagc taacgcagaa aacaatcacg aaatggatac tgaaaaatta tatatagcat
     4141 caatagttgc taatcaagga ccaacaatga agagattcat gcctagagct atgggtcgtg
     4201 caactacaat aagaaaaaga acttctcata tagaggttgt tgttaaagaa aaaaaataat
     4261 taatagatag gagggaaagt aatgggtcaa aaggttaatc cacacggctt aagggtcggt
     4321 gttataaaag attgggactc aagatggttc gctactgata aaaaagagtt cggaaactta
     4381 ttattagaag accataatat acgtaaattc ttaaagaaaa gattatactc agctggagtt
     4441 gctaagatag aaatagaaag atcagctaac aaaataaaaa tggacttaca cgttgctaag
     4501 cctggtgtag ttataggaag agctggtgca ggaatcgaag cattaaaagc tgaattagaa
     4561 aaaatgacaa agaaaactat aatagttaat atagtagaag taagaagtac agataaaaat
     4621 gctcaattag tagctgaaaa tatagcttta gctatagaga gaagggttgc tttcagaaga
     4681 gcaatgaaac aagctataca aagagcaatg aaatctggtg ctaaagggat aaaagtttct
     4741 gcttcaggaa gattaggtgg agctgaaatg gctagaactg aaggttatag tgaaggaaat
     4801 gtgccactac aaactttaag agcagatata gattatggtt tcgctgaagc tgatacaact
     4861 tatggaaaaa tcggtataaa agtttggata tgtaatggtg aagttttacc aactagagac
     4921 ggtgtaaatc caagagaaga aagcagaaag agcgatagaa gagacaacaa gagagataat
     4981 agaagaaacg atagaagagg aaatgacaga agaggaaatg ataacagagg aaactacaga
     5041 ggacaaagac ctcaaggtgg atcaagacct caaagaactg aaaacaaagg aaattaataa
     5101 tagttaggtt caaggaagga ggaaaaatac tcatgttaat gccaaaaaga gtaaagcgtc
     5161 gtagagttca tagaggaagc atggctgggc aagctcataa gggtaataaa gttacttatg
     5221 gtgagtttgg attagtagca ttagaagctt cttggataac ttctaatcaa atagaagctg
     5281 ccagaatcgc gatgactaga tatataaaaa gagggggaaa ggtttggata aaaatattcc
     5341 ctcataaacc agtaacaaga aaaccagcag agactcgtat gggtgcaggg aaaggttctc
     5401 cagaatattg ggtagctgta gttaagccag gaagagttat gtttgaatta gcaggtgttt
     5461 ctgaagataa agcaagagaa gctatgagac ttgctgcaca taaattacca atcaaatgta
     5521 aatttgttaa aaaagaagat ttagaagtaa agggtggtga ataggatgaa agctaaagaa
     5581 ttaagagatt taacaagcga agagctaatg aacaaattaa acgacttcaa aagtgaatta
     5641 tttagcttaa gattccaatt agctactggt caattagaaa atacagctag aataaagttt
     5701 gttaagaagg atatagcaaa agttaaaact gttcttgctg aaagaaagtt atacgaaact
     5761 agagcttaat ttggaaagga ggctttttaa acatggaaag aggaagaaga aaagttagaa
     5821 taggccgtgt tgttagtgat aagatggata aaacaatagt agtagctgtt gaagaattcg
     5881 tacgtcatcc actatataat aaacgtgtta agagaactaa gaaattcaag gctcatgatg
     5941 aaaagaatat atgtaacatc ggagatagag ttaaaataat ggaaactaga ccattatcta
     6001 aagataagag attcagacta gttgaagttg ttgagaaagt taagtagttt ttgaagaagg
     6061 agggattacg atatgataca acaagaatca cgtctaagag ttgctgataa ctcaggagct
     6121 aaagaacttt tatgtatacg tgttctaggc ggaagtaaaa gaagatatgg taacataggc
     6181 gacgttatag ttgctactgt taaaagtgca acacctggtg gagttgtaaa aaaaggtaag
     6241 gttgttaaag ctgttatagt aagaagcaaa caaggcgtaa gacgtaatga cggaagctat
     6301 atatcttttg atgaaaatgc tgctgttata ataaaggacg ataaaactcc agtaggaact
     6361 cgtatattcg ggcctgttgc tagagagtta agagacaatg aattcatgaa aatagtttct
     6421 cttgctccag aagtactata attaaggagg tgcaatagga catgatgcgt gttaaaaaag
     6481 gtgacactgt tgtagttata gcaggtaaag ataaaggtaa aaaaggttca gttttaaagg
     6541 tatatcctaa aactagcaaa gtgttagttg aaggtgtaaa tgtaataact aaacatcaaa
     6601 aaccaagtgc tatgaaccaa caaggtggaa taataaataa agaagcccca atacacatat
     6661 ctaatgtaat gccatttgat cctgaaacag gaaaaggcgt tagagtaaga tatgaagtaa
     6721 aagatgggaa caaagtaaga gtatcagcaa agagcggaaa agaattataa taaatattgc
     6781 ttgaaaggag ggaccttaaa tggcttctag attacaagaa aaatacatga aagaagttgc
     6841 tcctgcttta atggagaaat ttggatacaa aaacgtaatg gagataccta agttaaataa
     6901 aatagttatt aacatgggta taggtgacgc tagagaaaat ccaaaaggat tagaaaaagg
     6961 cgttgaagaa ttagaaatga tatcaggaca aaagcctgtt ataactaaag ctagaaaatc
     7021 tgttgctaac ttcaaattaa gagaaggaat gccaatagga acaaaagtta cattaagagc
     7081 tgacaaaatg ttctacttca tggacaaatt agtttcagtt tctttaccaa gagttagaga
     7141 ctttagagga gttaacccta atgcttttga tggtagagga aattatgctt taggagttaa
     7201 agaacaatta atattccctg aaatagaata tgataaaata gataaagtaa gaggaatgga
     7261 tataatattt gttactacag caaaaactga cgaagaagct cgtgaattat taaaattatt
     7321 aggaatgcca ttttctaagt aagcaaagga gggattcccg tggctagaaa agcgatggtt
     7381 gttaagcaac aaagaaagca aaagtacgct actagagaat acactagatg tactatatgc
     7441 ggaagaccgc attcagtttt gaaaaaattt ggtatatgcc gtatatgctt tagagaatta
     7501 gcttataaag gtcaaatacc tggtgttaga aaagcaagct ggtagaagct tgattagaat
     7561 ggaaggaggg caacaaaatg acaatgacag atccaattgc agatatgctt actcgtataa
     7621 gaaatgctaa tgttgttaag catgaaactg tagatgttcc agcttctaat atgaagaaag
     7681 aattagctag aatcttatta gaagaaggtt tcataagagg ttatgatgtt atagaagatg
     7741 gaaaacaagg tatcataaga atacaattaa agtacggaca agaaggcgaa agagttataa
     7801 caggtcttaa gaaaatatct aagcctggaa tgagagttta cgctgctaac catgagatac
     7861 caaaagtatt aaacggatta ggaatatcag ttatatctac ttcaaaagga atattaactg
     7921 acaagcaagc tagaaaagaa aatgttggtg gagaagtaat ctgctacgtt tggtaattaa
     7981 tatgaacaat gtaaggaggt gcgaccatgt caagaatagg tgttaagcca ataataatac
     8041 cagcaggtgt tgaagttact atagctgaag gaaatttagt tacagtaaaa ggtccaaaag
     8101 gaactttaac taaacaatta agtgctgagt taaacataaa aaaagaagaa aatacaataa
     8161 tggttgaaag accaactgat aataaaaaac atagatcttt acacggacta actagaactt
     8221 tattagataa tatggttgta ggtgtaaata ctggtttcga aaagaaatta gaattaaaag
     8281 gtgttggata tagagctcaa aaacaaggaa agaaattagt tatgaactta ggtttctctc
     8341 atccagttga gatggaagat ccagaaggaa taactgttga agctccaaac caaacagagt
     8401 taatagtaaa aggtatagac aagcaattag tagggaatta tgctgctaag ataagagctt
     8461 ggagaaagcc agagccatac aaaggtaaag gtataaaata cgttgatgaa gtaatcagac
     8521 gtaaagaagg aaaaactggt aaaaaataat accaaaactt aaacgctaga aaggagtgag
     8581 ctaggtgtta aaaaaggctg ataaaaatgc taatagactt caaagacata agagagttcg
     8641 tagaaaaata agcggtactt ctcaaagacc aagattatgt gtatttagaa gtgctaacaa
     8701 tatatatgct caaataatag atgacacaaa aagagtaact ctagttgctg catcttcttt
     8761 agaagcagaa gttaagagtg ctgtcaatca tacaggaaac aaggaagcag ctaagaaagt
     8821 tggagaatta gttgctaaaa aagctgttga aaaaggaatc actgaagttg tatttgacag
     8881 aggtggatac ttatatcatg gaagaattca agaattagct gaaggtgcta gagaagctgg
     8941 tcttaagttc taatacgaag gagggaaaaa tacatgctac gtcgtaagcc gatagatgca
     9001 ggacaacttg acttacaaga aaaagttgtt gaagtaagac gtgttactaa ggttgtaaaa
     9061 ggtggtagaa atttcagatt tgcagcatta gtagttgttg gagatgaaaa cggacacgtt
     9121 ggtataggtg ctgggaaagc aatggaagta ccagacgcta taaaaaaagc agttgaagat
     9181 gctaagaaaa atctaatagt agtgccaata gttgggacta caattcctca cgaagtacgc
     9241 ggacactttg gtgctggaaa tatattaata atgcctgctg tagaaggtac aggagtaata
     9301 gctgggggac ctgctagagc cgtacttgag ctagctggac taaaagacgt tagagctaaa
     9361 tctttagggt ctaacaatcc aagaaatatg gtaaatgcta caatagaagg acttaattct
     9421 ctaaaaacag ttgaagatat agctaaactt agaggtaaaa aagttgaaga acttctaggg
     9481 taaggaggta gtgtaaaatg gctaaattac aaataaagtt agttagaagt gttataggaa
     9541 ctactcctaa ccaaaaaaag aatgttgaag cgttaggatt aagaaaaaga gaacaagttg
     9601 ttgtaaaaga agacaatgct caaacaagag gtatgataaa taaagtaagt cacttattag
     9661 aagtaactga aatagctgaa taatttttat aaagtagtgc aaggaggtgc aatccatgaa
     9721 gttacatgag ttaaaacctg ctgaaggtgc agtaagagct aagagaagat taggtagagg
     9781 tactgcaact ggacaaggta aaactgcagg acgtggacaa aaaggtcaat ggtctcgttc
     9841 tggtggtgga gtaagagtag gatttgaagg tggacaaatg ccactagcta gaagacttcc
     9901 taagagaggt ttcaataaca tatttaagaa agtttacaca gaagttaatg ttgaagtttt
     9961 aaacagattt gaaaatggaa cagaaataac tgcagaatta ttaaaatcta ctaaaactat
    10021 aagcaaaata ggtaaagacg gaattaaaat cttaggtgaa ggtaatttag agaaagcttt
    10081 aactgttaaa gctgctaaat ttacagcttc agctcaagaa aaaatagaaa aagctggtgg
    10141 aaaagcagag ttagtataat aacctgctaa tctcctagcc agtacaggcg gggtgaatta
    10201 gcgtgctgtc aaaattaaaa caagcttgga agattaaagc agtaagaaaa aaagtaatgt
    10261 atacacttat gatgatcgtg atattcagaa taggtactac tataccagtt ccaggcatag
    10321 acactagtat tatacaaaag atggtaggcg gcaatagtct actttctctg tataatatgt
    10381 ttactggtgg agcatttagt aacttttcac tatttgcttt aggtataagt ccatatatca
    10441 ctgcatcaat cataatacaa cttctaacag taggttttga aagtttagca gaacttcaaa
    10501 aatctggtga agaaggaaag aaaaaaatca ataagtacac taaatataca gcactagctc
    10561 tagcagttgt acaagctttg ggaattacat taggaattgt aagaagtgct ttaatatcaa
    10621 atagtgtgtt ttttatcaca actgttgtaa taacattagt ttcagcaagt atgttagtaa
    10681 tgtggattgg ggacaaaatt actgaaaaag gaatcggtaa tggtagttca gtaataatat
    10741 ttgcaggtat aatatcaaga atacctacag atgttattaa aatatcgcaa caagttaaat
    10801 ctggcgaagt tgcaccttgg gttatagtga ttctagctgt agttatatta cttacagtaa
    10861 caggagttac atttatacaa gaagcaacta gaaagatacc tgtacaatat gctaaaagag
    10921 ttgttggaag aaaaatgtat ggaggacaaa gttctcatat accaatgaaa gttaaccagt
    10981 caggagttat gcctataatt tttgctagtt cacttttagc tttccctcaa acaatagcta
    11041 tgtttatggg tccaaatgct caggcttttg ttcaaaaata cttaagtatg gcaacagaac
    11101 aaggattttg gacatataga tcaattgaga ttctacttat aattttcttc tcatattttt
    11161 atacaactgt atcatttaat acagaagaca tatctaaaaa tatgaaaaat aatggtggat
    11221 ttataccagg tataagacca ggtgagccta ctatggatta tttaaataga attttaacta
    11281 gattaacttt agctggagct actttcttag caataattgc tatggtgcca gctcttacaa
    11341 cacattatat gaaagtaaat atgagtctag ctgggacttc attgctaatc gttgttggag
    11401 ttgctttgga gcttaagaga cagttagagt caaacttagt tatgagaagc tatcaaggtt
    11461 tcttaaaata aaatggagat ggataatatg agaataatat tacttggacc tccaggtgct
    11521 ggtaaaggta ctcaggcggc aggaatagta gaaaaataca atatacctca tatatcaaca
    11581 ggagatatat tcagaaagaa tataaaagag ggaacagaac ttggaaaaaa agctaaagaa
    11641 tacatggacc aaggtttatt agtaccagat gagttaactg taggtttagt tactgataga
    11701 atatctcaag aagattgtaa aaatggattt atgttagatg gatttccaag aaatgtagca
    11761 caaggagaac atttagatat cttcttaaaa aatgctggta tatcactaga taaagttgtc
    11821 aatattgaag ttgataagag tatattagtg tctagagcag ttggtagaag aatatgtaag
    11881 tcttgtggag ctacttacca tgttgagttt aatcctccta aagtagaagg tgtatgtgat
    11941 gtatgccaag gagaattata tcaaagagct gatgataatg aagaaactgt atctaagaga
    12001 atacaagttt atctagatga aactaagcct ttagtagatt attatagcaa acaaggtata
    12061 atagcagata taaaaggtga tcaagcaata gataaagtat ttgaagatat tgtcgcagcc
    12121 ttaggaagtg gaaaataaat gattatcttg aaatcaaaaa aagaaataga acttttaaga
    12181 gaagcaggta aaatagttgc tgacactcat gaagttttaa gaaaagctat ttctccggga
    12241 atatctactt tagaattaga taaaatagct gaagagaata taagaaaata caatgcagaa
    12301 ccttctttta aaggatatgg aggttttcca ggaagtatat gtgcttctat caatagagag
    12361 gtagtacatg gtattccagg agaaactata ttacaagaag gtgacattgt aagtttggat
    12421 ataggagcct attataaagg atatcatggt gattctgcga aaactcacgg agtaggcatg
    12481 atatctgaag aagataggaa attgatagaa gtaactagag aaagcttcta tgaaggaata
    12541 aaatttgcta aattaggata tagactttca gatatctcac acgcagtaca aacacacgtt
    12601 gagaaacatg gtttctcggt agttagagat ttagtgggtc atggagttgg agcaaatctt
    12661 catgaagacc cacaagtacc taattatgga ctcccaggaa aaggaccaag acttagagaa
    12721 ggaatggtta ttgctataga gccaatggtt aatgcaggtc gttatcatgt aaagactcta
    12781 tcagatgggt ggacgactgt tacaatagat ggaaaaaaat cagctcatta tgagcatacg
    12841 atagcaatta ctgaacatga acctttgata ttgacaaagt tgtaatttaa aaagtaggtg
    12901 aaaaaagtgc tatcagataa tttaagtata ggtcaagttg ttaaagtttc attaggacga
    12961 gataaaggaa atttattctt tgtagtcaaa ataattaata atgaatatgt attaatagct
    13021 gatggaaaaa agagaaaact tgacaaacct aagttaaaga aagtgaagca cttaaaaaaa
    13081 tataatttta ttaatgatga agttagaaaa agagtagtat ctggacaaga gattacagat
    13141 tcttttttaa gagctgaact tactaagtta aattagataa tttaacttat tgaaatggag
    13201 ggctggttag ttaatggcca aaaaagatgt tatagaatta gaaggtacag tttcagaagc
    13261 tttacctaat gctatgttca aagttaaact agaaaatgga catgagatat tatgccacat
    13321 ttctggaaag ctaagaatga acttcataag aattcttgaa ggtgacaagg tgaatgttga
    13381 actttctcca tatgacctta caagaggaag aattacttgg cgtaagaagt agactatatt
    13441 aatatttagt ctaaggaggg attaataatg aaggttagac catcagtaaa accaatatgt
    13501 gaaaaatgta aagtaataaa aagaaaagga aaagtaatgg ttatctgtga aaatccaaag
    13561 cataagcaaa agcaaggata agaaattatt actagcattt tgttaaattt tatagtataa
    13621 tactatattg tgatgttaac aaaagaacgt taaaatcgtt gaacaatctg gcgtaggatt
    13681 ggcatatgat gtgaagtttt aaacactaaa aactatgtgt aggaggtgcg atcatggcaa
    13741 gaatagctgg ggtagattta cctagagaaa aaagagcgga gataggctta acttatatat
    13801 atggtatagg taaagcaact gctaacgaaa tattagctaa ggctgagata aatccagata
    13861 ctagaataaa agatttatca gaagatcaag ttaatgagtt aagaaaagtt atagatgacg
    13921 atttcttagt tgaaggtgac ttaagaagag aaattgcttt aaacataaag agattaagag
    13981 atataaaatg ttatagagga ataagacacg ctaaaggtct tccattaaga ggacaaagaa
    14041 ctaagactaa tgctagaact agaaaaggtc ctagaaaaac agtatctcgt aaaaagaaaa
    14101 aataaataaa taataactag atgaaggagg gaaaaggaaa atggctaaac caaaaaagaa
    14161 agttacacgt attagaagaa gagaacgtaa aaatatagaa cgtggtcatg ctcatataca
    14221 atcaactttc aataatacaa taataacttt aactgacgtt cacggaaatg ctatatcttg
    14281 ggcaagttct ggacaattag gattcaaagg atcaagaaaa tcaactccat ttgcatctca
    14341 aatggctgct gaaacagctg caaaagctgc gatggaacac ggactaaaaa gtgttgaggt
    14401 attcgtaaag gggccaggtt caggaagaga agctgcaata agagctttac aagcaactgg
    14461 actagaagta actatgataa aagacgttac tccaatccca cataacggat gcagaccacc
    14521 aaagagaaga agagtgtaat taataagagt acaataggag gtgtagataa atggcaagat
    14581 atacaggtgc atcatgtaga caatgccgta gagagggaat gaaattattc cttaaaggtg
    14641 atagatgtta tacagacaaa tgtgctatag taaaaagaaa ctatgctcca ggtcaacatg
    14701 gccaaggaag aaagaaagtt tctaactatg gattacaatt aagagaaaaa caaaaagtta
    14761 aaagaatata tggagtttta gagactcaat ttagaaattt atatgaacgt gctgaaaata
    14821 tgcctggtaa agcaggtgag aacttattaa gtttattaga gagaagatta gataatgtag
    14881 tttacagaat gggactagca tcttctagaa aagaagctag acaattagtg actcatggtc
    14941 atttcacttt aaatggaaat aaagtagaca taccttcatt aatagttaag gttggagatg
    15001 ttatagaagt taaagagaaa tcaagatctt ctgcaaaatt caaaaattta gtagaagtta
    15061 actcaagaat agctcctaag tggttagaag ctaatgtaga aggaatgaca gcaaaagtag
    15121 ttggtgttcc aacaagagaa gatatagatc ttgagatagc agagcactta atcatagaac
    15181 tttactctaa ataataagca cacttttaaa aattatttta aaattttgtt taccctcagt
    15241 ggattactga attttaagga gggttttgtc catgatagaa atagaaaagc caaaagtaga
    15301 tatagttgag cttagcgaag actatagata tggtaagttt gttatagagc ctctagaaag
    15361 aggatatgga ataactatag gtaacgcatt aagaagaata ttattatcat cattaccagg
    15421 tgtagcagtg aatgctataa aaatagatgg agttcttcat gaattttcaa caatacctgg
    15481 tgttaaagaa gatgttactg agataatatt aactttaaaa gagctttcag caactataga
    15541 tggtgaagga agtagaacac ttaaaataga agctcaagga ccatgctcta tcacaggtgc
    15601 agatataatc tgtcctccag atgttgaaat attaagtaaa gatttagcaa tagctacatt
    15661 agatgataat gctaaactta atatggagat atttgtagat aaaggtagag gttatgtttc
    15721 tgctgaagaa aataaaacag agaatgttcc aataggtgtt ttacctgtgg attcaatata
    15781 tactcctgtt gagaaggtta gttatcatgt ggaaaacaca agagttggtc aaaaaacaga
    15841 ttatgataaa ttagtattag aagtttggac taatggtagt ataaatcctc aagagggaat
    15901 atcattagct gcaaaggtat tagttgagca tttaaactta tttatagact taactgaaca
    15961 tgtaagtagt gttgaaatca tggtagaaaa agaagaagat caaaaagaaa aagttcttga
    16021 aatgactata gaagaattag atttatcagt tagatcatat aactgtttaa agagagcggg
    16081 aattaataca gttgaagaat tagctaataa atctgaggat gatatgatga aggttagaaa
    16141 cctaggtaag aagtcattag aagaggtaat acaaaaacta gaagaacttg gattgggatt
    16201 aaaaccaagt gaagaatagc gagcaaagga gggataagaa tggctaagta ccgtaaatta
    16261 ggacgtgaaa cagctcacag aaaccttatg ttaagaaact tagtaacttg cttactaaga
    16321 agtggaagaa tagaaactac agtgactaga gcgaaagaaa ctcgtagaat ggctgaaaag
    16381 atgataactc ttgctaagag aggagatctt catgctagaa gacaagtttt agcttatgtt
    16441 atggatgaaa cagtagttaa taacttattc acagatttag ctccaaaata tgctgagaga
    16501 aatggtggat acactagaat aataaaaata gggccaagaa aaggcgatgc tgctgaaatg
    16561 gcttttatag aattagtata gtaaggaaag gattaggctt gatggcttaa tctttttttt
    16621 atttttttga ctaaggtatg atataataat gtgtaaaatg atttttataa gttattgctt
    16681 actaaaagga gtatgagtta atggataata tagtaaaggt aaataatatt tcgtttgagt
    16741 atattacaga tgaagcaaaa cttaaagcaa tagataattt aagcctagat gttaaaaaag
    16801 gagaatttgt tgcgataata ggacataatg gttctggtaa gtctacttta tctaaaaact
    16861 taaatgctat tcttatgcct actgaaggaa atattcttat tgatgatatg gatactaaag
    16921 aagaagaaag attatgggat ataagacaaa ctgcaggcat ggtatttcaa aatccagata
    16981 atcaaatagt tgctactata gtagaagaag acgttgcatt tggacctgag aacttaggta
    17041 tagaaccaaa agaaataaga cgaattgtag aggagtcttt aaaaagtgta ggtatgtatg
    17101 atttaagaga tagacagcct catttgttat caggaggtca aaagcaaaga gttgcaatag
    17161 caggaattat tgctatgagg cctaaatgta taatatttga tgaagcaaca gcaatgttag
    17221 atccatctgg aagaaaagaa gttatgaaaa caataaaaag acttaataag gaagaaaata
    17281 taactgttat acatataacg cactttatgg aagaagcagt agaagctgat agagttgtag
    17341 ttatggaaaa aggaaagaaa atcttagagg gaactcctag agaagtattc agtaaaataa
    17401 agatgttaaa ggaaatcggt ttagatgtac cttgtatgac agagttatct agtttattaa
    17461 tagaagaagg tataaatatt agtagtgata tattaactgt ggatgagatg gtgatggaat
    17521 tatgtcaatt atagtaaaga atttaacaca tatatataat gaagggatgc cttttgcaag
    17581 taaggcactt gatgatgttt cttttgagat taaagataga gattttgttg gtcttatagg
    17641 tcatacaggc tcaggaaaat ctacattaat acaacattta aatggattac taaagccttc
    17701 ctcaggagaa atatttataa atgattttaa tataacagat aaaaacttga atttaactga
    17761 gattagaaaa agagtagggg ttgtatttca atatccagaa taccaattgt ttgaagaaac
    17821 gattgataaa gatatagctt ttggaccctc taatttaggc ttagaggagt ctgagataca
    17881 taacagggta aaagcatcaa tggaagctgt aggacttgat tatgagggat ttaaagacaa
    17941 atctcctttt gaattatctg ggggacaaaa gcgtagagta gcaattgctg gagttatagc
    18001 catgaaccca gaagttctca tattggatga accaactgct ggtcttgacc ctggtggaag
    18061 agatgaaatt tttaacttaa taaaagactt acatgaaaaa aagaatatga ctataatact
    18121 atcatcacat agtatggatg atatggcaaa gttagcaaaa actttaattg ttatgaatca
    18181 tggtagcgta gaatttatgg gaactcctag agaagtgttt aaatcaaatg ctagtaaatt
    18241 aaaagatata ggattagata ttccacaagt tttagaactt gcattgaaat tgagagaaaa
    18301 aggctttgac atcagtgagg atatattgac tttagaagaa gcaaaacagg aaatattaaa
    18361 agttgtgaga ggacgaggat tatgttaaaa gatataacta tagggcaata ctacccaaca
    18421 agttctgcta ttcataaatt agatccaagg ataaagcttg tagcgacaat tgtatttatg
    18481 gtatctatat ttgtagtaaa taaattttgg ccttatatag tagttttatt atgtttatta
    18541 gctatgataa aattggctaa tataccagtc aaatatattg ttaagggtgt taaacctctt
    18601 aaatggatta ttttgtttac atttcttata aatatttttt tcttaccagg tgatgaaata
    18661 tggtcatttg gatttttagc tattacaaaa cagggtctaa gacaagcaat ttttatggca
    18721 ataagattaa tatttttagt agttggtaca tcattgctta ctttaacaac ttcaccaata
    18781 gaattaactg atggaataga gagattgttg aatccattta agaaaattgg tttgccagtt
    18841 catgaacttg caatgatgat gacaattgct ttacgtttta ttcctacatt gttagatgag
    18901 acagacaaaa taatgaaggc tcagatgtct agaggtgcag attttgaaag taagaatctt
    18961 ataaatagag caaagaattt agtaccactt ttggtacctt tatttgtaag tgcatttaga
    19021 agagctgatg aattggctat ggccatggag gccagatgtt acagaggtgg acataatagg
    19081 actaagatga gagagtctgt tatatcaaga agggattata tggcatgtgt atttcaggtt
    19141 gtttatctgg gagcaattat agctactaga tttatagcaa tttaattaat aagttataga
    19201 tataatgagg taaagtatga gaaatataaa aattaaaatc caatacaatg gtaagaatta
    19261 ttgtggatgg caaaagcaac ctgattcttt agggatacaa ggtacaattg aaagggctat
    19321 atatgatata acaaaagaag aaaccagtct tatagggtct ggtagaactg attcaggagt
    19381 tcatgctatt ggtcagattg caaattttaa aattaattca ggtataagta tagaaagtat
    19441 accaatggca ctaaatgcca agttgccaaa agacatatca gttatagaag catgtgaagt
    19501 aaatgatgat tttcactcca gatacagtgc aaaaggtaaa acatataaat atttagtata
    19561 taatagcaag tttagaaatc caatacttag tgagatatca tatcaagtaa aatatgaact
    19621 tgattttgat aaaatgtgtt cagaagcaaa aagtttatta ggaacacatg attttaaagg
    19681 ttttatgagt tctggttcat ctgtaaaaga tacagttaga acgatatatg atattgatat
    19741 aagtaaaaaa gatgacttga ttacttttga gattagtggt aatggttttt tatataacat
    19801 ggtgaggatt attgttggaa cacttgttga tatgggtcgt ggcagaatta acgaaccatt
    19861 tttagatata atacaatcaa aaactaggtc aagatgtgga catacagctc cagcacaagg
    19921 attatttctc aaaaaagttc attattaact tgacacacca ggctacatgt attaaaatat
    19981 atgagaatgt gttaataagt ccacttgccc cggacttgac ataaacggta aaaaaatttt
    20041 atgaaaagta gttaaggagg gacagttatg aaaagttata tagctaagcc agctgatgta
    20101 caaagaaaat ggtacttagt tgatgctgaa ggaaaaacat taggtcgttt agcaacagaa
    20161 attgcgacag tattgagagg taaacataaa ccaacattta ctcctcacgt tgatggtgga
    20221 gattttgttg ttgtagtaaa tgcagaaaaa atagttttaa gtggtaagaa attagatcaa
    20281 aaatattaca gataccatac tggttatgta ggtggattaa aagaaatatc ttacagagat
    20341 atgatggata agaaaccaga agaagttata tcacatgctg taagcggtat gttacctaaa
    20401 aataaattaa gaagcagaat gatgactaga ttaagagtat ttgcaggtgc tgagcacact
    20461 catgcagctc aaaatccaga agttttaaac tttaaataag attaataggt gaaaggagga
    20521 gtttataatg gctaacgttc aatattatgg aactggaaga agaaaaagtt ctgttgctag
    20581 agtaagacta gttgcaggtg aaggaaatat attagtaaat ggaagagcat tagaaaatta
    20641 ttttaattat gagacattaa taagagatgt taaacaacca ttagttttaa ctggtaatga
    20701 aaataaatat gatgttatag taaaagttga aggtggagga ttcactggac aagctggagc
    20761 tataagacat ggaatatcta gagctttatt aaaagctgat ttagatttaa gacctgcttt
    20821 aaagaaagaa ggtttcttaa ctagagatgc aagaatgaag gaaagaaaga aatacggatt
    20881 aaaagcagca agaagagctc cacaattctc aaaaagataa ttacttattt ttttggaaaa
    20941 gcaaaatcct ttatttactg ttattcagaa agtattggta ttttaaaaat gttgggttaa
    21001 gttaattatt gatatataat aaaataataa gatatcttat taggatgttt ttcattaaga
    21061 agaacatcct ttttattttg actatttaat aaaaaacata tgctaataag atttaaattt
    21121 tatttttata atattgtaat ttaaaagttt atataaggtt atatatttta agatattatt
    21181 taacaatata aattctgaat atacgttatc aaagtttata atataaacta agtataattt
    21241 attgtatagt attgaatgtt agataaacat agtgaattaa ataggggcag aaatataata
    21301 tcattacata ataatatatt aataccaata aataaagata tatcaatatt aaaaaaattt
    21361 aaatgtttgt actaatatat ggaatattat agtgttttta taagtataca tatacagggt
    21421 gatgttgtga gaaagtacat aaaacatata atttttagtt ttgcaatgat atgtctagta
    21481 gtagtatcca tatttgaaat taaaaatatt tctgaagatg ttatcaagta tatgccagta
    21541 acaaacaaaa ctataatttt agatgctggt catggaggca ttgatccagg tgcattaaat
    21601 aaggataaga gtacatctga aaaggatatt aacctagcaa taacacttaa gcttagagag
    21661 cttatagaat caagtggtgg ccttgtaata ttaactcgag aagatgacag tagcctttat
    21721 aaagaagaaa ataataaaac tacaagacaa aaatataatg agaatttgaa aaatagaaaa
    21781 gaaataatat ctaattctaa tgccaatatg tttgtctcta tacatcttaa tgcatttgaa
    21841 caatcaaaat actatggagc tcaaactttt tatcctaaag acaaacaaga cagtaaagaa
    21901 ttatcgaagt gtattcaaga agaacttaaa agagtagtag ataagacaaa caacagagaa
    21961 gtcaaaccaa gggatgatat atatcttttg aaagaaaata atattccatc agtactgata
    22021 gaatgtggtt ttttatcaaa tgaaaaagag tgtaaactct taactgatga aacatatcaa
    22081 gaaaaaatag catgggcaat ctacatagga atacaaaaat atttaagttg aaataactat
    22141 aaaaataggt ctaaatatgt aaatagcttg tattaaagca gttaaaatgc attaatatag
    22201 gctattttta ttttgacaaa aaaatattta aaataaaagt taaaaagttg ttgacttaga
    22261 ataatataga tgatattata tatgagtgcc caaaaggagc accaaaataa gac
//
LOCUS       DAEPIN010000061        21981 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.061, whole genome shotgun sequence.
ACCESSION   DAEPIN010000061 DAEPIN010000000
VERSION     DAEPIN010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21981
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.061"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            218..439
                     /locus_tag="KR250_002746"
     CDS             218..439
                     /locus_tag="KR250_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat associated protein CotJA"
                     /protein_id="HBH0068667.1"
                     /translation="MGYLKEDKQDYCCEFARPYINRQIYDDTYNPKTALKRGTLFPEL
                     DLIESNNYNDWLYANPKNRVRAKKGGNMY"
     gene            439..702
                     /locus_tag="KR250_002747"
     CDS             439..702
                     /locus_tag="KR250_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein CotJB"
                     /protein_id="HBH0068668.1"
                     /translation="MSLNRRELLERISEYQFACIELNLYLDNNPRDKKALDSYNRYCD
                     KFTQAVCDYESKYGALTNFGYESSEYPWSWISEPWPWDKSFYK"
     gene            764..1336
                     /locus_tag="KR250_002748"
     CDS             764..1336
                     /locus_tag="KR250_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HBH0068669.1"
                     /translation="MWIYQKTLEHPVNIRQADPRMAKYIMTQLGGPNGELAAATRYLQ
                     QRYTMPTGKSRALLTDIGTEEMAHVEIISSVLYQLIGNCTPEELKAAGLGSNYANFGH
                     GLQPVDSNGVNFTTSYINVFGDSVTDLHEDMAAEQKALATYYQLINLTDDPDLKDILR
                     FLGEREVVHYQRFGEALMDVYEFTECKHQF"
     gene            complement(2090..2440)
                     /locus_tag="KR250_002749"
                     /pseudo
     CDS             complement(2090..2440)
                     /locus_tag="KR250_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729451.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2757..3290
                     /locus_tag="KR250_002750"
     CDS             2757..3290
                     /locus_tag="KR250_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HBH0068670.1"
                     /translation="MQYISYYHSPIGNILLASDNIGLTGLWFENQKYYAYNLDQEHQE
                     KELPIFEQTKKWLDIYFSGKEPDFTPSLHMVGTPFQTTVWKILQQIPYGKTITYGEIA
                     YKVAQQKGISKMSAQAIGGAVGHNGISIIIPCHRVVGTNGSLTGYAGGINRKIKLLSL
                     EKVSVQHYFIPKKGSAL"
     gene            3516..3695
                     /locus_tag="KR250_002751"
     CDS             3516..3695
                     /locus_tag="KR250_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBH0068671.1"
                     /translation="MLQTGKQGADKKYLDNMFFVKGYVEELLFLDNNYNIVFSCIVFH
                     HCTNVQNIFCIFWKW"
     gene            complement(4303..4920)
                     /locus_tag="KR250_002752"
     CDS             complement(4303..4920)
                     /locus_tag="KR250_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="HBH0068672.1"
                     /translation="MDEKILELLQEMQGSISGIEGTISDMQSNINGIEGTISDMQSNI
                     NGIEGTISSMQGNINGIENTISGMQGTIGDMQGSISGMNGTISDMQGSISSMDGTINS
                     MQDSITDMQVEIKEVNSKLDNMEPDIKDLKTGQVETSSRLDTIQSDVTEIKSDVKINR
                     NTLDTVIKHTSSLVKDTSDIKNDLNNIEIVTSKNSNDIAKLKSVK"
     gene            5386..5799
                     /locus_tag="KR250_002753"
     CDS             5386..5799
                     /locus_tag="KR250_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003507530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HBH0068673.1"
                     /translation="MKMPKESIDTIMFAPCGMNCMVCYKHCYHKKPCAGCLNGDTGKP
                     EHCRKCKIKDCVCQKGLFYCFECFNFPCKLIKNLEKSYNKRYQASLMENSKFVQQYGL
                     EKFMQKQKERYTCSKCGGIISIHDRECSECQEKVK"
     gene            5872..6408
                     /locus_tag="KR250_002754"
     CDS             5872..6408
                     /locus_tag="KR250_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HBH0068674.1"
                     /translation="MIGLKCGMVKLLEHQMIWDKSAKDVIILLKSIWDKTAIDIQHIG
                     STSIPSISAKPIIDIVVGVASLEEAKLYLERLEQCGIVFRGQDVPKQLLFAMGDFEKN
                     TRTHHIHVVEWNGVAWNNYINFRDYLNTFTDKAKLYDSHKQKLALQFEDNRKIYTQRK
                     QELINILLEEARLWRINT"
     gene            6568..7230
                     /locus_tag="KR250_002755"
                     /pseudo
     CDS             6568..7230
                     /locus_tag="KR250_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016440163.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
     gene            7776..7895
                     /locus_tag="KR250_002756"
                     /pseudo
     CDS             7776..7895
                     /locus_tag="KR250_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            8022..8924
                     /locus_tag="KR250_002757"
     CDS             8022..8924
                     /locus_tag="KR250_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HBH0068675.1"
                     /translation="MLPQDYYGLIVILGVILGVMGAFYNWFTLYVQSLYKKIPKIGTF
                     GKLLIPFIMAGILGLVMPDVLGSGHNLVEELTSHEYIFGMVVLIFVIRFIFSAVSFGS
                     GAPGGIFFPLLVLGAFIGGIFGMVGVKVFGMNPDYVNNFVLLAMAGYFTAIVRAPLTG
                     IILIFEMTGSVSQMLSLSVISIVAYIVATLMKSKPIYESLLERLLEKNGEPVHKERGE
                     KILDQFSVMFGSPVANKAIQDVQWPENCLLVAIQRGGEEIIPRGKTILRPSDVIVTMT
                     DERDSGAVYDKMENLCKEHTSHIF"
     gene            9536..10549
                     /locus_tag="KR250_002758"
     CDS             9536..10549
                     /locus_tag="KR250_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HBH0068676.1"
                     /translation="MVSSFLNDIFRTILVSCIFIVILLVFRITLFKIFSKKFNYYIWL
                     IVIIKLSLPFMNYTFIFNESKYNGNINKISLGNFNSISIVYSTVLVSVWIAITIFYLV
                     YTLLKYIKLKNMINDLSYDVEDDKIKNLYENLLKELKINKKIRLKYSYEVESPVFFNS
                     CVILPPSDYTLKELNWIFRHELMHFKSKDLYIKYLVIFLKCVYWFNPFVYIMEKFIDL
                     DCEFYCDERVLKNCTIEEKQDYALTIISAMRKGSNSSNKFVAGLHKESDIKKRVFSMF
                     NEKYRNGILIAVILCLLSSITYFKIDLITTKSIFYPLSKQTPIENRTNSKPTYQINVY
                     IDN"
     gene            10905..11474
                     /locus_tag="KR250_002759"
     CDS             10905..11474
                     /locus_tag="KR250_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HBH0068677.1"
                     /translation="MKKKLEDMTLEELWNLFPIFLTTHKECWKNWFKEEQYRLTGILP
                     MQKIVRISHIGSTSVSTIYAKPIVDILIEIIPDYNMKDIKDLIESNGYICMSESSSRL
                     SLNKGYTVDGFEERVFHLHIRYVGDNDELFFRDYLIDHPDVAKEYEKLKLDLWKKYEH
                     NRDAYTDSKLDFVREFTEKAKVLYFNRYN"
     gene            complement(11810..13147)
                     /locus_tag="KR250_002760"
     CDS             complement(11810..13147)
                     /locus_tag="KR250_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBH0068678.1"
                     /translation="MGTYSLISTSINFILILINIFIAFFLYTLTSYFIDFRNHWIFKI
                     ILILGYSIISQMIIWLSDPVNILFTLFGYFILLIICSKSKLIPIISVVMIFYPIIIGL
                     NFLFINNPIYEELIHSKEYILILSIVQIISALIKLLFWFLIHIFLKNRLVNIKQYLSN
                     KIWIFIDMVCISSFLSILIAIILPPTNLIPMGSNKNIILGNLPYGTYLIILSAILANI
                     GVILLLQPLIENVKMKIEKQANHLKEKYYNLLENQQIQIRKMRHDMNNHFQMIESYLE
                     IEDIAGAKKYFNQLKIGIGTLSGKQFCKNQALNSILNLRHTSLEENGVDVHFNIDIDN
                     HLGIEPIDLCTIFSNSLDNAIEASLKIPDLSNRKVVLKARCEKEYFSYLLTNNKVNPI
                     NRKQELFISDKKDSNSHGYGIENIREIVNKYNGKLDISYTENEFSLFLYIRLF"
     gene            complement(13546..14256)
                     /locus_tag="KR250_002761"
     CDS             complement(13546..14256)
                     /locus_tag="KR250_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0068679.1"
                     /translation="MTSICICDDEKQQRNMLNKIITREMDLWGYPYQIKEYSNGESLV
                     YNLEHENYYIDIIFLDIELGKSNGVDIAKTIRKHSKDTIIIFVTGFSDYVFHGYDVGA
                     LNYILKPYKTEKICKVLKEALKKLEQWKENFITVQIGTNLCKINTKDILYFKSELRKL
                     TVVTFDKTLEYYGKLNDMETVLPSNFIRTHQRYIVNLNHVDSITQTYAKVQNEKIPIS
                     RKKYQEVVGKFTRYMLEP"
     gene            complement(14640..16751)
                     /locus_tag="KR250_002762"
     CDS             complement(14640..16751)
                     /locus_tag="KR250_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0068680.1"
                     /translation="MTINKLYSKLRAYNWKNYTTLFFCIILSVVLVTSYALIYFSPTI
                     QNILPTGGDSRKQATMIFSVAILGCAVFTTYASSLFLKFKSKEIGIFMALGTDKKKLK
                     KLLFSEILLISIVCCFLGLILSIPVSFGIWKIFQLVIIDTREMTYHFGTQGFIYGFIF
                     SVFVTLCIFILSVKFINRTNIIDILNDQRISEPIQDVKDWYGILGIIFIILGIFLGYL
                     VPSLTINKLNYKLPSIWSFTYLLSLIGIYMIMTYIVVHAKRGKHPEKYYKNIISTSMM
                     RFMGRQTVKNMCVISFLIAGALFAVFYVPNMVTGIYDTLKNNPIDYTFTYNQRDKQVS
                     KKDIEQLADKHNIKIQNYMEIPSIELIVDGVESIYHKDGTIEKVYMKKSSYDMFFNSK
                     DFSKLIGKPITIKNGEYLAVVNKGNENQDYKYTKFISSPVTEKEIPFRYAGKIIFNGS
                     FFQNRSMNAYVLNDEDYKSFTKDLSIKNYNNSILFNVDKDTYAFANDLKKEIIKRTSK
                     EMGMFSGYDEYVKKRYESTGRKYFMDEEYPSGEGHLKLDPNNNQLYLNWKYYPNFKIL
                     QSQDMIKNMAVFLMLFIYVAIICFTAVGIIAYTRGVTIAINHKQVFMDLNRLGANNDY
                     IKFCIRNQLKKVFSYPLIAGSFLIYLFNFMIMLNNDGRIVNSEVNALLINLGFIGICG
                     VYMYLIYLLTYKKFKKIIGIL"
     gene            complement(16741..17493)
                     /locus_tag="KR250_002763"
     CDS             complement(16741..17493)
                     /locus_tag="KR250_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0068681.1"
                     /translation="MLKVNQLCKSYKTGSKTYPVLKNISFEINKGEFVAIMGSSGSGK
                     TTLLNCISCFIPIDNGEIILGNNNIRNLKEKELSEVRNQQLGFVFQDFMLFDGLSVFE
                     NICVPQIIKGSQVLQMENRAINLCELFGIEHIRKKYPAEISGGEKQRTAVARALMNNP
                     DLILADEPTGNLDSKSCRTVIESFLKAKQELDATILMVTHDSFAASFCDRVIVLKDGI
                     IYKEVVKSNSRHKFLDELLEVLKEMGGAQDDN"
     gene            complement(18759..18935)
                     /locus_tag="KR250_002764"
     CDS             complement(18759..18935)
                     /locus_tag="KR250_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0068682.1"
                     /translation="MFFTFIGVISAILLLCTIICGLWIKLNNRNNDSNSHFTFSILSI
                     AFSLLIVVAFLVRN"
     gene            complement(19039..19647)
                     /locus_tag="KR250_002765"
     CDS             complement(19039..19647)
                     /locus_tag="KR250_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBH0068683.1"
                     /translation="MGKLLRGKIMKADSTKEQLINATIQLLSSHRDVSSITAREIVAK
                     ANVNLAMINYYFKSKEELINISIKKFLKRHMDEYKATHDTKNLAPKQQLREILIAFFD
                     FIGEHSQFIKISVPYILIQEEIVYPFEILPLIKAHYENQNNKKTEAEYRTIAYQITSF
                     IQLLFLRSNAFFKYSGIDILNIEQRNGLIDFQLDLLLVDITK"
     gene            complement(19746..20570)
                     /locus_tag="KR250_002766"
     CDS             complement(19746..20570)
                     /locus_tag="KR250_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBH0068684.1"
                     /translation="MFKIGEVSKLTQISIRMLRYYDELGILKPAEIDKYTGHRLYSVE
                     QISILQRIVLLRDSKFSVAEIANIVHNWNDEFVIKELNRKKNEIQKEINQEQQRINKI
                     DKFIEAINCDKDEIHYNVVFKKIPSYKIISLREIIPDYQSEGILWEKLSKFIKEEHIE
                     VSRQSNNNIAFYHDEEVKDNGVDIEVGMVVKKIGKNKSGFIYRETEEIDMMACTMVYG
                     PYENIAGAYESFCYWLDKNSDYQISGINRQIGHKGEHNEINPENYLTEIQIPLIKV"
     gene            complement(20748..21641)
                     /locus_tag="KR250_002767"
     CDS             complement(20748..21641)
                     /locus_tag="KR250_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBH0068685.1"
                     /translation="MQLYNTPFNTNKHLIYFFTFTLVLNWIFSLTPALLGITNTILGE
                     IIFYLAYTSPAIVGLFFISTIYPQSAKQDFIYRYLSFKEIRIKWILLTILFFALTFIT
                     SLIIGNYFNVKTSDIDWIHIAVFRPSKIAYMLLASFILSILSQEAGWRGYAIDKLLVR
                     FGFIGSSIILGLVCGIWYLGSYFTPNQIPYNLAQYSLFDAFLFIPNIVLLNFIINFVY
                     INTSRSILSAGLVHMMYYFFNIQLLLHYPIKLCVIAQYVQIIFGLIFLVYMISSNKFK
                     QKVNSEIEQIKSDDFEFELDW"
BASE COUNT         8007 a         2636 c         3337 g         8001 t
ORIGIN      
        1 taaaaaattg tataaactct atattattta tttaaaatag atatttatga aagattaatt
       61 agaaaaattt tagaggtaaa ataagaaaaa aagatactta tttaatcatt taatcttatt
      121 aaagacctat taaaactacc agttataata tagaaccagt atacaatttt taaaaataat
      181 gctattttaa aattctaaaa attaaggagg ataattaatg ggatatttaa aagaggataa
      241 acaagactat tgttgtgaat ttgctagacc atacatcaat cgtcaaattt atgatgacac
      301 atacaatcca aaaacagcat taaaaagagg tactttattt cctgaattag atttaataga
      361 gtcaaataac tataatgatt ggctatatgc aaatcctaaa aatagagtta gagctaaaaa
      421 agggggtaac atgtactaat gtcattaaat agaagagaac ttttagagag aatttcagaa
      481 tatcaatttg cctgtataga attaaattta tatttagata acaatccaag ggataagaaa
      541 gctcttgatt catacaacag atactgcgat aaatttacac aagctgtgtg tgattatgaa
      601 tctaaatatg gagcattaac taattttgga tatgaatcaa gtgaatatcc ttggtcatgg
      661 atatctgaac cttggccatg ggacaaatca ttttataaat aaaattatta aactttaaaa
      721 ttagatttat tattaaatat atattaagga ggagtaagat aatatgtgga tttatcaaaa
      781 aacactggaa catccagtta acataagaca agcagaccct agaatggcaa aatatatcat
      841 gactcagttg ggaggaccta atggtgagtt ggcagctgca actagatatc ttcaacaaag
      901 atatactatg ccaactggaa aatctcgtgc acttttaact gatataggta cagaggaaat
      961 ggctcatgtt gagataattt cttcagtgtt atatcaatta ataggcaatt gtactccaga
     1021 agagcttaag gctgctggac ttggtagtaa ttatgctaat tttggacatg gtcttcagcc
     1081 agtagattct aatggagtaa actttactac aagttatatt aatgtctttg gcgattcggt
     1141 aactgattta catgaggata tggctgctga acaaaaagca ttggctacgt actatcaatt
     1201 aataaattta actgatgacc ctgatttgaa agatatattg agatttttgg gtgagaggga
     1261 agtagttcac tatcaaagat ttggtgaagc attaatggat gtttatgagt ttacagagtg
     1321 caagcatcag ttttaatgaa gaattgaaag tgaatggaga ataagcatca tttttaatga
     1381 agaattaaaa gtgaatggag agtaagcatc atttttaatg aagaattaaa agtgaatgga
     1441 gagtaagcat aagttctaat gaagaattaa aagtgaaaag agaataagca taagttctaa
     1501 tgaagtatta aaaatgaaaa gagaataagc atcattttta atgaagtatc aaaaataaaa
     1561 agagtgtaaa tattagctct gataaactat caaaataaga ggtaaaagtt ttagttgatt
     1621 tcttttatct cttatttttt attgattaaa cagattactt tgctatttaa atatattatt
     1681 ttagcaaaaa aagtccaatc aaaagttcaa agtattacca tagttcaaag cattgccgtt
     1741 gttagtaagt tttttacaat attttttatt tactctttgg ataatcaaaa gaaataaaaa
     1801 atcatatctc tttttttata tttctgtaca cttttggaga atatcccgtt ttttctttaa
     1861 aacatttact aaaatagatt atttgatgaa aaacaacttt tatagtaatg tcacttatta
     1921 gctcatttgt gccttttaga aattgagttg cttttaataa acaaaactca gtaaaatatt
     1981 tataaggtat agtatttaaa cttagttcaa atcatctgga gcactctgat ttattgatat
     2041 taccactgct tgatagtact ccaaaattaa gttttcacaa tagtaccctt caatataatg
     2101 taaaacaata gtatttttca atataacgta aaattttttg catatggata ttaattatat
     2161 ttttgtgttt tattggtggc aaaacaatat tttttatcat aataagccaa aggaaagaaa
     2221 cagaaactaa tgcttcatac atatacagtt catttttact tttttctaac cttattaatc
     2281 gttgtagaat agaaaacact ttattgcgtc aattatattt atgtgtaaaa tgataaatag
     2341 caaattgttc gttttctgtt attctatcta ctaacgattt tactggattt ctaacataaa
     2401 aatacttttc ttttttacag cctattctta cttttcccat ttgaaagtat tatgatatta
     2461 cttgatgcta tatcgatatt tttttggtct atttagtgat aaaattataa gatacacttc
     2521 tataaaagtt aatacagtag gaagcttaaa tgtatgaatt ttatatccat caaagggaaa
     2581 gggaaaaaag gatgaacaga tgcaggtaaa tataaaacta atatgatatg aggatagata
     2641 ttataacgaa agtgtagtta gaaggaaatt atattagtat gtttttacat aatggtagat
     2701 agaaaagaag tgtctaatga tttgagtgaa gttttcagaa tataaggagg aattgtatgc
     2761 aatatattag ctattaccat agtccaattg gaaatattct tttagcatca gataatattg
     2821 ggttaactgg tctttggttt gagaatcaaa agtattatgc ttataatctt gaccaagaac
     2881 atcaagaaaa agaattgcca attttcgagc agacaaagaa atggttagat atttattttt
     2941 caggaaaaga acccgatttt acaccatcac tccatatggt aggtactcct tttcagacta
     3001 cagtgtggaa aattttacaa caaataccat atggtaaaac aatcacttat ggagaaattg
     3061 catataaagt tgctcaacaa aaaggaatat ctaaaatgtc agcacaagca attggtggtg
     3121 cagtagggca taatggaatc tctattatta ttccatgtca tcgtgttgta ggaacaaatg
     3181 ggagtttgac tgggtatgct ggtggaatta atagaaaaat aaaattgtta tctttagaaa
     3241 aagtatctgt gcaacattat tttatcccaa agaaagggag tgcattataa agccattaaa
     3301 tttagaaatt cttaatcatt tatttgatat acaaacatct aattttgaaa gcaataactt
     3361 gcaattttaa aaaatgatta tttaaactat atgttataca accttattcc tagtaaacat
     3421 tatacaactt tagaggtagt ttttagagct tgtattttgg aagtttattt tcttttttca
     3481 tgtgagtagt tatatgttta gaggaagtaa tttatatgct acaaacagga aaacaaggag
     3541 cagacaaaaa atacttagat aatatgtttt ttgtgaaagg ttatgttgaa gaacttctat
     3601 ttttagacaa caattataat attgtatttt catgtattgt atttcatcat tgtacaaatg
     3661 ttcaaaatat attttgtata ttttggaaat ggtaagagtg ttaaatgtaa gggaaaaatt
     3721 tataatgatt gatacttaag ctactcatta agtattaaga accatacaag ataaggttga
     3781 aatttatatg tttcgtttta tgtaaaaaat gtaagtgagt tagaaatgaa agaattattt
     3841 ataaaaaata gattaagtat tggaaaatat gaaaaataga tgttgaacaa aacttagtcg
     3901 attagttaaa tttaaccaaa cacttaaagt atcaaaagaa aaaattatta ctcttaagca
     3961 agaagatata aatggaaaaa tgaaaacaag attttatcct tttataagtg ataaggggat
     4021 gtgttttaat caaagatgga cttttatttt aggctgaaaa attatataac tatattatag
     4081 gatataaggg agatacacaa ctttttagtg tccaatctcc cttatttttt attattatat
     4141 caagactcat ttagtcgtgt taattttagg ataaaatgag tattttttta ataaattatt
     4201 tataagtaaa gtgtgttgtc ggataatcag tatttctgta taaaatttgg tataacttta
     4261 aagaagtatg tgaaattttc aggtattttt tactatggta tattatttaa ctgattttaa
     4321 tttagcaata tcattcgaat tcttagatgt gactatctct atattattta aatcattttt
     4381 aatatctgat gtatctttta ctaatgaaga ggtatgttta atcactgtat ctaatgtatt
     4441 tctgttaatt tttacatctg acttaatttc tgtaacatca ctctgaatag tatcaagtct
     4501 actactagtt tcaacttgtc cagtttttaa gtctttaata tcaggttcca tattgtctaa
     4561 cttactattg acttctttaa tttcaacttg catatcagta atactgtctt gcatactatt
     4621 aatagtgcca tccatactac tgatactgcc ttgcatatcg ctaatagtgc cattcatacc
     4681 actgatacta ccttgcatat cgccaatagt gccttgcata ccactaatag tgttttcgat
     4741 accattgata ttgccttgca tactactaat agtgccttcg ataccattga tattgctttg
     4801 catatcgcta atagtgcctt cgataccatt gatattgctt tgcatatcgc taatagtgcc
     4861 ttcgatacca ctgatactac cttgcatttc ctgtaataac tctaatattt tttcatccat
     4921 cactatcaca ctcctgtttt attattataa cataatttag gtgttatatt ccacaaacgc
     4981 tatgtcatat ttaattttta atcatatgat tatctgctat tcaaaacatg gttaaatata
     5041 tctatattga gaactttttt attaatgaat cttatgtatt gtctaagttt ctaagatact
     5101 gtaataagtg tttttgaggc atgtgtggtt atatttgtat ttaaaactgc tggtaatatc
     5161 atatactttt aaatatacct taagttattt atattgatta cttgtatttg aataatttga
     5221 ttagtagctt cctttttatc tctttttatt cttagtttta aactttcata tttccttgaa
     5281 aatttattgg caatgaaagt gcatgaattt tttagctatt ttttatgtta caatatgaat
     5341 atataacaat tcgacaaatt ggatttttga aaggagtaaa atgttatgaa aatgccaaaa
     5401 gagagtattg atacgattat gtttgctcct tgtggaatga attgtatggt ttgctataag
     5461 cattgttatc acaaaaagcc atgtgctggt tgtttgaatg gcgatacagg aaagccagaa
     5521 cactgccgta agtgtaaaat aaaggattgt gtctgtcaaa aagggttgtt ttattgtttt
     5581 gaatgtttca attttccttg taaactcata aaaaatcttg aaaaaagtta taacaagaga
     5641 tatcaagcaa gccttatgga aaatagtaaa tttgttcagc agtacggttt agagaagttt
     5701 atgcaaaagc agaaggagag atatacttgt tctaaatgtg gaggaattat atctattcat
     5761 gatagggaat gtagcgagtg tcaagaaaaa gtgaaatagc taaatcactg tttatcaaga
     5821 gagaaaaaat tgtaatatat ataaaggtgg attatattga ggtggtatat agtgataggt
     5881 ttaaaatgtg gtatggttaa attattggaa catcagatga tatgggataa aagtgcaaaa
     5941 gatgtcatta tattattgaa aagtatatgg gataagactg ccattgatat tcaacacatt
     6001 ggaagcactt ctattcccag tattagcgca aagcctatta ttgatattgt tgtaggtgtt
     6061 gcttctttag aggaagctaa attatacctc gaaaggttag agcaatgtgg aatcgttttt
     6121 cgaggtcagg atgtaccaaa acaattactt tttgctatgg gagattttga gaaaaatact
     6181 cgtactcacc atattcatgt tgttgaatgg aatggtgttg catggaataa ttatatcaat
     6241 tttcgagatt atctaaatac ttttacagac aaagcaaaac tctatgatag tcataaacaa
     6301 aaattggcat tacagtttga ggacaatcgt aaaatctata cacagagaaa gcaagaacta
     6361 ataaatattt tgctggaaga agcacgactg tggagaataa atacctaaaa gaataaattt
     6421 acatttattg agaaattgag caattatttt cttatattta gtaaataaaa atctattttt
     6481 gttgattaaa aaatatctgt acacactcat gactttagtc attggagatt cagattgtgt
     6541 agaattattg gatgaaggag gaacagtatg aaaaaggata catctcatat tatcaaacgg
     6601 gcagagcact ttcaggtaat tttgattggt gaaggactta tggttggggc tgtggctggt
     6661 ttgattgtgc ttttatacag gatactttta gggcaggctg gtacttggct tagccaaatt
     6721 ttaaaatttg tagaaggttc tacaataaaa ataatagctt ggtttgcagt acttgcagta
     6781 cttgcatgga ttgtatcaaa gttggtggac tgggagccaa tgatttctgg aagtggtatc
     6841 cctcagcttg agggggaaat gacagggaaa ataaaacaag tatggtggcg agtgttgcca
     6901 acaaaattta ttggaggatt cttatcactc atgggtggtc ttgctttggg aagggaaggt
     6961 ccttccattc agcttggtgc tatggcaggt aaaggtattt ctagagctct tgaccgaggg
     7021 aagacagagg aaaaattcct gttaacctgt ggggcaagtg ctggattatc agcagccttt
     7081 catgcaccac tggcgggtgt tatgttttca cttgaagaag tccataaaaa cttttcagta
     7141 tctgttttaa tttctgtgat gacagcatct ttgacagcag actatatttc agccaatttc
     7201 ctaggaatgg agtctgtatt ccagtttgat taaaaaatag ctacgcacac ttgtgacttt
     7261 agtcatgagt tagtagctaa aatagtcagc atatagggaa acctatatgt agagatagga
     7321 tagaacctat ccaatactac ttgaattgct ggaaacccct aaagctaacc aaactaaaac
     7381 ataaggatga aataaaccta agtgtgaaag ttacgaaagt agaaaaaatt ggttagatgg
     7441 tataaggtta aatcctaagt actttaaaat gggcaatcag caggtaaact ccgaatagga
     7501 gaaacttcaa cgactattcc tcttgaggga agtacactac aagctattgg tagtggaagt
     7561 gggtagacct taacggataa tgccgaagga taagatatag tctgtgcttg catgaaagtg
     7621 taagaagttc ataagagaac tgcataggta gtagcgaact tatgtgaacg acaacctcaa
     7681 aaatgataaa agttaagact tacggttctt aaatatgtta tttaaaatta aatataaaac
     7741 cttctaaata attgttttaa ataattacat ttgttaacat tattataaga gaatggattt
     7801 gtcctagttg taatgaaact cacgatagag atataaatgc aagtataaat atattaaaag
     7861 aaggattaag actaataaca attcaaaata aataacttat gtaagaaccg taggaactac
     7921 ggggatagcc tgtggagaat tagtaagaca tatgttagta tgcaaaattc tatgaagcag
     7981 gaaccctgtg actttagtca tgggaggttc agattggaaa tgtgttgcca caagattatt
     8041 atggattaat agtgatttta ggtgttattc ttggagttat gggggcattt tacaactggt
     8101 ttactctata tgtgcagtct ttatacaaga agattcctaa aattggtacg tttggaaaac
     8161 tgttgattcc atttataatg gctggtattc ttgggcttgt tatgcctgat gttctgggga
     8221 gtggacacaa tttagttgaa gaacttacaa gtcatgaata tatttttgga atggttgttt
     8281 tgatttttgt tatacgtttt atattttcgg ctgttagttt tgggtcaggt gcaccagggg
     8341 gaatattttt tccattgctt gttttaggag catttatagg tggaatattt ggtatggtag
     8401 gtgttaaagt ttttggaatg aatcccgatt atgtaaataa ctttgtgctt ttggctatgg
     8461 caggatattt tacagctatt gtgcgtgccc ctttgactgg tatcatatta atttttgaaa
     8521 tgacaggttc tgttagtcaa atgctttcac tatcagtaat ttcaattgtg gcatatattg
     8581 tggctacact tatgaagtct aaacctattt atgaaagtct tcttgagagg ttacttgaaa
     8641 aaaatggaga acctgtacat aaagagaggg gcgaaaagat attagaccag ttctctgtta
     8701 tgtttggctc tcctgttgcg aacaaggcaa ttcaggatgt acagtggcct gaaaattgtc
     8761 ttttggtagc aattcaaaga ggtggagaag aaattattcc tagaggtaag acaattctta
     8821 gaccaagtga tgtaattgtg actatgacag atgagagaga tagtggtgct gtgtatgata
     8881 agatggaaaa tttgtgtaaa gaacatacgt cacatatttt ttagggataa tgatgaatgt
     8941 ttaataatga tagaatattt aatgaagatt tgattaaaaa attggtaatt tgaaagagag
     9001 ttaaggaatt aatataagaa aatgaaaatg tgatatctta gatgtaaaag agtttgaaga
     9061 aaataattgt atatatttgt taatgagatt atatggacta atggtatatt tatactgtgc
     9121 taaaagttgc actattgtat gaggaatggg aataaagggt ttgaaaaaat aggtagaaga
     9181 tttttcagat attcttactg ggatatttta attaatatat tttaattgcg atattttggg
     9241 aagaatagaa tttatttata aatgaaaata attaaaaggg gttaaggttg tattagcccc
     9301 ttttgcttat agagtttgga aatggtatct tgatagcagt tgtttcatgc ttattatatt
     9361 ttattacata ttttaaaatt gaccttatta ctcctaaatc attgttatat caacttttaa
     9421 aacactttca agacaatcgc ctattgaaaa caaagttaat taaaaaccta catatcagtt
     9481 tagtattcgt gtagatgatt atataaatat aaaagtataa gaaaggtgag tatctgtggt
     9541 tagttcattt ttaaatgata tctttagaac aattttagta agttgtattt ttatagttat
     9601 tttattagtc tttagaataa ctttatttaa gattttcagt aaaaaattta attactatat
     9661 atggcttatt gtgattatta agctatcatt accatttatg aattatactt ttatatttaa
     9721 tgagtcaaaa tataatggta atattaataa gataagttta ggcaatttta atagtatttc
     9781 tattgtatat agtactgttt tagtctctgt ttggattgct attacgatat tttatttagt
     9841 ttatacttta ttgaaatata ttaagcttaa aaatatgata aatgatttgt cttatgatgt
     9901 tgaggatgat aagataaaaa atctttatga aaatttatta aaagagctta agataaataa
     9961 aaaaatacgg cttaaatatt catatgaagt tgaaagtcca gtatttttta attcgtgtgt
    10021 cattttacca ccaagtgatt atacattaaa agagttaaac tggatattta ggcatgagtt
    10081 gatgcatttt aagagtaaag acctttatat taagtatttg gtgatatttt taaaatgtgt
    10141 ttattggttt aatccttttg tgtatataat ggagaagttt atagatttgg attgtgaatt
    10201 ttattgtgat gaaagagttt tgaagaattg tacgatagaa gagaagcagg attatgcatt
    10261 gacaataatt agtgcaatga gaaaaggttc aaatagttca aataaatttg tggctggatt
    10321 acataaggag tcagatatta aaaaaagagt gtttagtatg tttaatgaaa agtacagaaa
    10381 tggtattttg atagcagtta ttttatgttt attatcttct attacatatt ttaaaattga
    10441 tttaattact actaaatcaa tattttatcc actttcaaaa caaacaccta ttgaaaacag
    10501 aactaattca aaacctacat atcagattaa tgtttatata gataattaaa aaatacaaag
    10561 tttaacaaga gattaatata aataatttta ataagtatct ttcacatatg ttaaatttac
    10621 tttaattata ttaaagccaa tgaattattc tttagaagct ccaatgccag taaatacagg
    10681 taaagaaaaa tattttacta caattgcaga ttaaaaagtg ttctaatatt taaagaaata
    10741 cacaagtgta agaagggtaa aaatctgtta taatacatta aaaactaaat ttagaaaaat
    10801 ttacataatt agaaaaatga tttgataaaa aataaagtta aatattaact aagtaaattt
    10861 gaaaaagaaa aaatataaag tacaaattta aaaggaggta actaatgaaa aagaagttgg
    10921 aagatatgac tttagaggag ttatggaact tgtttcctat atttttgact actcacaaag
    10981 aatgttggaa aaattggttt aaagaagaac aatatagact aactggaata ttgccaatgc
    11041 aaaaaattgt tagaataagt catattggaa gtacttctgt gagtacgatt tatgccaagc
    11101 ccattgttga catactgatt gagataattc ctgattataa tatgaaagat attaaagatt
    11161 tgattgagtc gaatggatat atttgtatgt ctgagagcag tagtaggctt tcacttaata
    11221 aagggtatac agtagatggg tttgaagaac gagtttttca tttgcatata aggtatgtag
    11281 gtgataatga tgagttgttt tttagagatt acttaattga ccacccagat gttgcaaagg
    11341 agtatgaaaa acttaagttg gacctttgga agaagtatga gcataatcgt gatgcttata
    11401 cagatagtaa actagatttt gttagggagt ttactgaaaa ggcaaaggtg ttgtatttta
    11461 atagatataa ttagaaatag gtacaattag atatttatct tatatatcgt attatatttt
    11521 ttaagaaagt aattaataga aaaaggggtt gtttcaaaat ggagagataa atcccttttt
    11581 cattgttgaa aggataaaaa tatatttgat ttcatgtaaa aaagagtgcc tcatcaacta
    11641 aaaagtttat tttgagacac acacattttt actaaggtaa ataaaatatc acatttgatt
    11701 aaattttaag ttaatgaata tatgtgtaaa tatttaatta taagttatta taattttaca
    11761 tagtatcaaa caatctaatt tgtattatat aagataatct aattttagac taaaataatc
    11821 taatataaag aaataaagaa aattcattct cagtataaga gatatccaat tttccattgt
    11881 acttattaac aatttctcta atattctcaa taccatatcc gtgagaatta ctatcttttt
    11941 tatctgaaat aaatagttct tgttttctat tgataggatt taccttgttg tttgtcaata
    12001 aatagctaaa atactccttc tcacaacgtg cctttaatac cacttttcta tttgacaaat
    12061 caggaatctt caaagatgct tctatagcgt tatctaaaga atttgaaaaa atagtacata
    12121 aatctatagg ttctataccc aagtgattgt ctatatctat attaaagtgc acatcaacac
    12181 cattttcttc caaacttgta tgacgaagat ttaaaattga gttaagagct tggtttttgc
    12241 aaaactgttt gccactaaga gtacctatac ctatttttaa ctgattaaaa tactttttag
    12301 ctccagcaat atcttcaatt tctaaatacg attcaatcat ttgaaagtga ttattcatat
    12361 catgtcgcat tttgcgtatt tgaatttgtt gattttctag taagttatag tatttttctt
    12421 ttagatgatt tgcttgtttt tctatcttca tctttacatt ttcaatcaat ggctgtaaaa
    12481 gaagtatcac accgatattt gccagaattg ctgacaagat gattagataa gtgccataag
    12541 gtaagttgcc taagataata ttcttattac tacccatagg gattaaatta gtaggtggca
    12601 gaataatagc aattaatatt gataaaaatg aagatataca taccatatca ataaaaatcc
    12661 atattttatt tgataagtat tgttttatat tgaccaatct attctttagg aaaatatgta
    12721 ttaaaaacca aaataataat ttaattagag cagaaattat ctggactatt gaaagtatta
    12781 aaatatactc ttttgagtgt ataagttcct catatatagg gttgtttata aaaagaaaat
    12841 taagcccaat tataatagga taaaatatca tgacaactga tattatagga attaatttgc
    12901 ttttgctaca tattataagt agtataaaat atccgaatag tgtaaacaaa atatttacgg
    12961 ggtcggaaag ccatattatc atttgactaa taatagagta gcccaaaatc aatatgattt
    13021 tgaaaatcca atgattccta aagtctataa aataggaagt caacgtataa agaaaaaagg
    13081 ctatgaaaat attgattaat attagaataa aatttatgct tgtagagatt aaagaataag
    13141 tgcccattta atataccttt ctattaatta agattctgga aactaagttt taaaaaatta
    13201 aatcttaaaa attaaaattc tggaaattaa atttcaaaga attgattttg aaaaataaaa
    13261 ttctagagat tgaacttcaa aaaattaaat ttgaaaaaaa actctaaaaa ctaagcttca
    13321 aaaaattaaa ttttgaaaga ttgaaattct agaaactaag ctttaaaaaa ttaagtttga
    13381 aaaaacaaaa ctctaaaaac taagcttcaa aaaattaaat tttgaaagat tgaaattcta
    13441 gaaattaatc ttcaaaaaat taaatttgaa aaaacaaaac tctaaaaact aagctttaaa
    13501 aagttaagtt tcaaaaataa aattctagag attgaacttc aaaaattaag gttctaacat
    13561 ataccttgta aattttccta caacctcttg atatttttta cggcttatag gaattttttc
    13621 attctgaact ttggcataag tttgagttat actatctaca tggtttagat tgacaatata
    13681 cctttgatgc gtacgtataa aattagaggg aagaacagtt tccatatcat ttaattttcc
    13741 gtagtattct aatgttttat caaaggtaac tacagttaat tttcttagct cacttttaaa
    13801 gtagaggata tcttttgtat taattttaca aagattagtt ccaatctgaa ctgtaataaa
    13861 gttttctttc cattgttcta atttttttag tgcttctttt aatactttac atattttttc
    13921 agttttataa ggttttaaga tgtagttcaa agctcctaca tcatatccat gaaatacata
    13981 gtcagaaaaa cctgtgacaa agatgattat agtatccttt gaatgttttc gtatagtttt
    14041 tgctatgtca acgccattac tttttccaag ttcaatatct aaaaaaataa tatctatgta
    14101 ataattttcg tgttctagat tgtatactag actttctcca tttgaatatt cttttatttg
    14161 gtaggggtag ccccacaaat ccatttctct tgtaatgatt ttattcagca tgtttctttg
    14221 ttgtttttca tcatcacaaa tacaaatcga agtcaatttt ttatctcctt tcaaatttta
    14281 tatttatata gttaagtata taatatattt tgctacctag atatataaaa tgaaataaca
    14341 attatatcca gatagcaaaa taaaattttt tatattagta tacttacact taaaactatt
    14401 ctattaaact tattctgaag tgctaatgat tttcttatta aaaaatctaa caatctttct
    14461 tattataaaa tacaaatgct tttattatta taaaaagtaa tgattttatt actttaaaaa
    14521 tctaataacc tttcttatta taaaacacca atgattttat tactttaaaa atctaataac
    14581 cttttttatt ataaaacacc aatgatttta ttactttaaa aatctaataa ccttttttat
    14641 tataaaatac caataatttt tttgaatttc ttataagtta atagatatat aaggtacata
    14701 taaaccccac aaatccctat aaaacctaag tttatcaaaa gagcattgac ctcactattg
    14761 acgattctac catcattatt taacataatc ataaaattaa acagataaat taaaaaactt
    14821 cctgcaataa gagggtatga aaatactttc tttaattgat ttctaataca gaactttata
    14881 taatcattgt ttgctcctaa tcgatttaaa tccataaaga cctgcttgtg atttatagct
    14941 atagtaacac cccttgtata tgcaatgatt cctactgctg taaaacatat gattgccaca
    15001 tatatgaata acattaagaa gactgccatg ttttttatca tatcttggct ttgtaaaatt
    15061 ttaaagttag gatagtattt ccaattaaga tacaactgat tgttgtttgg gtctagtttt
    15121 aaatgacctt caccacttgg atattcttca tccataaaat attttcttcc agttgattca
    15181 taacgttttt ttacgtattc atcataacca ctgaacatgc ccatttcctt agatgttctt
    15241 tttataattt ctttctttaa gtcattggca aaagcatagg tatctttatc tacattaaat
    15301 agtatagaat tattgtaatt ttttatggat aaatctttag taaatgattt ataatcctca
    15361 tcattaagga cataggcgtt catacttcta ttttgaaaaa aagaaccatt aaatattatt
    15421 tttccagcat atctaaaggg gatttctttt tctgtgactg ggcttgatat aaatttggta
    15481 tatttataat cttgattttc atttccttta tttacaacag caagatattc accatttttt
    15541 atagttattg gtttgccaat tagcttggaa aaatctttag aattaaagaa catatcatag
    15601 gagctttttt tcatatatac cttttcgata gttccatctt tatgatatat tgattctaca
    15661 ccatcaacaa ttaattctat agaaggaatt tccatatagt tttgaatttt tatattgtgt
    15721 ttatctgcta attgttcaat atcttttttt gatacctgtt tatctctttg attgtaggta
    15781 aaagtataat ctatagggtt atttttaaga gtatcataga tacctgtaac catattagga
    15841 acatagaata cagcaaataa tgctcctgca atcaaaaatg aaataacaca catgtttttt
    15901 actgtttgtc ttcccataaa tctcatcatg ctagttgata tgatgttttt atagtatttc
    15961 tctggatgtt ttcctctttt agcatgtaca actatgtatg tcataatcat atatattcca
    16021 ataagagaga gtagataagt aaagctccat atagatggta gtttataatt taatttgttt
    16081 atagttagtg aaggtacgag atatcctaga aaaataccga gtataataaa aattatacct
    16141 aatataccat accagtcctt tacatcctgt attggttcag atattctctg gtcatttaat
    16201 atatcaatta tatttgtacg attaataaat tttacagaaa gtataaatat gcataatgtc
    16261 acaaatacac taaatataaa tccatatatg aatccttgag ttccaaaatg atatgtcatt
    16321 tctctggtat caataatcac taattgaaat attttccata taccaaaaga tacaggaata
    16381 cttaatataa gacctaaaaa acagcatact attgaaatta acaatatctc tgaaaataga
    16441 agttttttta atttcttctt atctgttcct agagccatga aaataccaat ttccttgctt
    16501 ttaaatttta aaaagaggga agaagcgtat gtagtaaaga cagcgcatcc taatatagca
    16561 actgagaata tcattgtagc ctgttttctg ctatctccac cagttggaag tatgttttgt
    16621 attgttgggc taaaataaat tagagcatat gaagttacta ataccacaga aagtattata
    16681 caaaagaata aagttgtata gttcttccaa ttataggctc gaagtttact gtataattta
    16741 ttaattgtca tcttgagcgc ctcccatttc ttttaaaact tctagtaatt catctaggaa
    16801 tttatgtctt gaatttgatt ttacaacttc tttataaatt attccatctt taagcacaat
    16861 aactcggtca cagaagcttg ctgcaaagct atcgtgtgta accatgagta tagtagcatc
    16921 taattcttgc tttgctttta aaaatgattc aatgactgta cgacaagact tactatcaag
    16981 gttaccagtt ggttcatctg ctaatattaa atctgggttg ttcatgagtg ctcttgctac
    17041 tgctgtacgc tgtttttctc caccagatat ttcggcaggg tatttttttc tgatatgttc
    17101 aataccaaat agctcacata gattaattgc acggttttcc atttgtaaga cttgactacc
    17161 tttgataatt tgtggaacac atatattttc aaagacacta agaccatcaa atagcatgaa
    17221 gtcttggaat acaaatccta gttgttgatt tctaacttct gaaagttcct tttcttttaa
    17281 atttcttata ttattatttc caagaattat ttccccatta tcaattggga taaaacatga
    17341 aatacaattt aaaagagtag tttttccact tcctgatgaa cccatgattg cgacaaattc
    17401 tcctttgttg atttcaaaag aaatattttt taatacagga tatgttttac tgcctgtttt
    17461 atatgattta cataattggt ttactttaag catatatatc atcctcactt tcatttatct
    17521 tgtaagtcaa tgatagcatt tgaaagggga ggagggatat gagctatatt gtaatagttg
    17581 gatgtttttt tgtaatgttt gaaaatatat tgtttggata gtgtgagagg ttattattat
    17641 agttaaattg attatattat tggaggattt agagggttat ttttaggtat taattgttgt
    17701 gctaaagtaa tatgatgtgg aacattgaag gtgtagttag aaaagaagta tatagtaaaa
    17761 gtaaaaaggt tatagaataa cgaatgttgt attgaaaaaa atgatgtaaa taatgtatag
    17821 tagggttaaa aagaaaaaaa gaattataga gcagtaagat tatttaatag aatgtattgt
    17881 aaataatttg aaaggattta ggggctatta acacaatgta attgagaaaa tatattggag
    17941 ttttggaaat aaataggttg ttagaaacaa ttgaaatgta taataaaaag gttaaaaagt
    18001 attattagta taggtatgat agataatttt agaaagtgga agtattttag actatgctga
    18061 aaagtgattg atatgatttt gagatattaa aatattacta tattatagta aagaagttaa
    18121 aataataaaa tgttgtaata ataaaggttt gtagtataat aaagaactgt aataaataag
    18181 actttgcaat gtaatagaaa tgctgtaata ataaaagtct gtagtataat aaagaactgt
    18241 aataaataag actttgcaat gtaatagaaa tgctgtaata ataaaggtct gtagtatagc
    18301 aaagaactgt aataaataag agattgtaat aataaaagtc tgtagtgtaa taaagaactg
    18361 taataaataa gactttacaa tgtaatagaa atgctgtaat aataaaggtc tgtagtataa
    18421 taaagaactg taataaataa gactttacaa tataatagaa atgttgcaat aacaaaacac
    18481 cataataaat aagagtttgc aatataatag aaacactgca ataaataaga gatttcaata
    18541 taataaaagt ctgtaatata acaaaatcct ataataaata agattttaca atataataaa
    18601 aatcctataa taaataggac tctgtaatat aataaaatgc tgtaacaaca aaacactata
    18661 ataaataaga ccctgtaata taataaaaat actataatat agtaaaaatc atgcaataat
    18721 aaaaatctat aataataaac ctcagcataa caaagacatt aatttcttac taaaaaggcc
    18781 accacaatta acagtgaaaa agcaattgaa agtattgaaa aagtaaaatg agaattagag
    18841 tcattattac gattattaag ttttatccat aaaccacata taattgtaca caacaataaa
    18901 attgctgata ttactcctat aaatgtaaaa aacattaaaa ttcctccccc ttatttaatg
    18961 caagtatttt tagtcaatga gaatattttc aaacgttacc ataaaataaa aaaatgtaca
    19021 taaaaaatta atatagtatt attttgtaat atctaccaat aataaatcta attgaaaatc
    19081 tattaaacca tttctctgtt caatattaag aatatcaata ccactatact tgaaaaaagc
    19141 attagaacga agaaaaagca actgaataaa tgaagttatt tgatacgcaa tagttctgta
    19201 ttcagcttca gtttttttgt tattctgatt ttcatagtga gccttaatta aaggtagtat
    19261 ctcaaatgga taaacaattt cttcctgtat caatatatag ggtacagaaa tctttataaa
    19321 ttgactatgt tcacctataa aatcaaaaaa tgcaattagt atttccctta attgctgttt
    19381 aggtgctaaa tttttagtat catgtgtagc cttatattca tccatatgac gctttaaaaa
    19441 ttttttaata gaaatattaa ttaactcctc tttagattta aaataataat taatcatagc
    19501 taaattgaca tttgctttag ctacaatttc tctagccgta atgcttgaaa catctctatg
    19561 tgaagatagt aactggattg tggcattaat taattgttcc tttgttgaat ctgctttcat
    19621 aatttttccc cttaaaagtt tccccataaa aaaattaaac atgtttaaca cacgtttaga
    19681 tactatcatg aaaattttgg gatgtcaatg ggtgattagt acttaagtat ttaagtacta
    19741 atctattaga cttttataag ggggatttga atttctgtaa gataattttc aggatttatt
    19801 tcattatgtt cacctttatg tccgatttgt ctatttatac ctgatatttg ataatcacta
    19861 tttttatcta accaataaca gaacgattca tatgcaccag ctatattttc atatggacca
    19921 taaaccatag tacatgccat catatcaatt tcttctgtct cacgatatat aaatccagat
    19981 ttgttctttc caatcttctt tacaaccatt ccaacttcaa tatctacacc attatctttg
    20041 acttcttcat catggtaaaa agctatgtta ttgttggatt gacgtgatac ttctatatgt
    20101 tcttctttga taaatttaga tagcttttcc cagagtatac cttcagattg gtagtctgga
    20161 attatctctc taagtgatat gattttataa cttggaatct ttttaaagac aacattgtaa
    20221 tgaatttcat ctttatcaca gtttatagct tctataaatt tatcaatttt atttattctt
    20281 tgttgttcct gatttatttc cttttgtatt tcattctttt ttctattcaa ttctttaatt
    20341 acaaattcat cattccaatt atgtacgata tttgctattt ctgcaacgct aaattttgaa
    20401 tctctcaata atacaattct ttgtagtatt gaaatctgtt caactgagta taatctatga
    20461 cctgtatatt tgtctatttc tgcaggtttt aaaataccaa gttcatcata ataacgcaac
    20521 atacgaatag aaatttgtgt tagttttgaa acctctccta ttttaaacat gaaaatatga
    20581 cctccttttc acttataatc agtatatatt ttgtttttat atacttcaag cgtttgtgga
    20641 aatacaatta ttttccagat gttgcaataa attatagaga gtaaattata tttaagtagt
    20701 aatcgtatca aatcatagat tatcttaatt tataattcat attaaaatta ccagtctaac
    20761 tcaaattcaa aatcatccga ctttatctgt tcaatttcag aatttacctt ctgtttaaat
    20821 ttattagaag aaatcatata tacaagaaat attaaaccaa atattatttg tacatattga
    20881 gctattacac ataatttaat agggtagtgt agaagtaatt gaatgttgaa aaaatagtac
    20941 atcatatgaa ctaacccagc tgataaaatg cttctacttg tattgatata tacaaaatta
    21001 attataaagt ttaaaagtac aatgtttggt ataaataaaa aagcatcaaa tagggagtat
    21061 tgtgccaaat tatatggtat ttgattaggt gtaaaatacg accctagata ccagattcca
    21121 caaacaagcc ctaaaataat tgaagagcct ataaatccaa atctaactaa taatttgtca
    21181 atggcatatc ctctccatcc agcttcttga ctaagtatac tcaatataaa acttgctaat
    21241 agcatataag ctattttaga aggtctaaac actgctatgt gtatccaatc tatatcagaa
    21301 gtttttacat taaagtaatt tccgattatt aacgaagtaa taaaagttaa agcaaaaaat
    21361 aatattgtta atagtatcca ttttattcta atttctttaa agcttaggta tctataaata
    21421 aaatcttgtt ttgcactttg tggatagatt gtagaaataa aaaatagacc tacaatagct
    21481 ggtgatgtat atgcaagata aaaaatgatt tctccaagta ttgtatttgt aattccgagc
    21541 aacgcaggtg taagactgaa tatccagttc aatactagcg taaatgtaaa gaaatagatt
    21601 aagtgtttgt ttgtattaaa gggggtgtta tatagttgca ttttttcact atccttccgt
    21661 tttataatga agttttaatt tttattaatt gtttatttat ttttatatgt attaagttat
    21721 cgcttgatgt tagttattgc ctataataat atcctatttt ttatattttg attaattatt
    21781 tttacactaa atataattaa ttagtattta atattgaata tattatatta ttattaatta
    21841 ttaaataggt caataaattt gcaattagtg atattaataa attactttag atttttatta
    21901 aatgtttagc ttttctttaa tattttttat ataacgacga tttataataa ctttttcgtt
    21961 gttttttaaa gtagcttgca t
//
LOCUS       DAEPIN010000062        21842 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.062, whole genome shotgun sequence.
ACCESSION   DAEPIN010000062 DAEPIN010000000
VERSION     DAEPIN010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21842
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.062"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            <1..210
                     /locus_tag="KR250_002768"
     CDS             <1..210
                     /locus_tag="KR250_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0068686.1"
                     /translation="AMSELRSLIKQYDEMIHKDWNLATEEQKNRVEKLKCEVDNLKKS
                     DTGDDSKIWVEAIQNIAMKRGVNNG"
     gene            203..1612
                     /locus_tag="KR250_002769"
     CDS             203..1612
                     /locus_tag="KR250_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HBH0068687.1"
                     /translation="MDKALLTLLDCYWDNPVWFAEDMLNFKADKWQSDVLMALAQTPK
                     VSIRSGQGVGKTGLESIATVWYLSTRPFPKVVATAPTRQQLYDVLWAEIAKWLSNSKV
                     EKLLEWTKTKVYMKGFEERWWATARTAVKPENMQGFHEDYMLFVVDEASGVADPIMEA
                     ILGTLSGAENKLLLCGNPTRTSGTFYDSHNRDRDLYKTFKVSSLDSPRTSKDNIEMLK
                     RKYHEGSDPWRVRVLGEFPKGESDSLISLEAVETSTIREVNISNDYILNIGADIARYG
                     DDETIIAPRIGGKVFDLLTYSKKDTMETVGNILRAVDKFKSMYHQINRVKIKTDDDGL
                     GAGVTDRLKEVIRQERLKYEIVPIQNGSSAIEKDKYYNKASEMWDNMREELDANLSGF
                     IQNKEAIIQLPNDDKLIKQLSNRKYTVDSKGKIQIESKKEMKKRIGESPDRADAVIYS
                     FAENNNTDLSLLKGGSVWG"
     gene            1603..3105
                     /locus_tag="KR250_002770"
     CDS             1603..3105
                     /locus_tag="KR250_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBH0068688.1"
                     /translation="MGIISYVKKLFKRPTGEIMRMSSGNIGVYKLDDSRVDYELAREL
                     YQNKNANYKLGSSFVRPIVNSTTGFMGVPHFQIEDEEAQYILDEFVLDNTSKMLKTHT
                     DSLKQGDCYIWITREERENPLYPDKKVRLIYNFISPEEVKEIILDPTTKEPIAYILES
                     QNEWTDLEENKRRAKVKQIITAESRIIEVEGDKIEGLEEGETPNVWGFIPIIHFKNEA
                     DETLKYGQSDIEPIEPLLKAYHDVMLHALKGSKMHSTPKLKLKLTDVASFLAHNFGVE
                     DPVKFAKEGGKINLDGHEILFLNKDEEAEFVEVKSAIGDAKELLKLLFYCIVDVSETP
                     EFIFGVHTPSALASVKEQMPIMVNKIRRKREQFTNSWQLLARMVLIMSSNSSGMKYSS
                     YDVTIGWDEVNPRDDKELAETLEKVCSALDKALEGGFISEESTVNFLAQYIDTMSNYI
                     SDDPEIVGEREKIIKTKMLKYRLDDSQGLDDESNEIDKEIDKIKDNNDNG"
     gene            3098..4105
                     /locus_tag="KR250_002771"
     CDS             3098..4105
                     /locus_tag="KR250_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0068689.1"
                     /translation="MDKSTLELITVAGEYKKWALEARKKFIDLRLKQDDEIRTMYINI
                     TRNITKEIRKGNLSDFNKVRLKQILKQLTQEIKILNEQLVFNFDEYLNKNVETATSYS
                     KNILINAVETAQITKVTKAMIQKAFYDINIRTVEAYYTRVKDGLFLSDRIWSKCKKYR
                     EDMKVILQTAVTEGQDCVKTAKMLDKYVLKGKKTLVDEYPNMIKRIGNRVPQNISYEA
                     LRLARTEMTSAYGDGVLASAMINPATIGIQFMLSMAHPHTDICDEICGEDNFGLGKGV
                     YPINEAPAYPFHPHCLCIMLTVVQPLDILVGRLKNWIKNPMNDTPLEMWYQEVYGNLN
                     F"
     gene            4125..5459
                     /locus_tag="KR250_002772"
     CDS             4125..5459
                     /locus_tag="KR250_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0068690.1"
                     /translation="MNVITGEMDSMNALISSIKPSDIPLAKDIDIEALKSIDDDPLEV
                     VVEIPATKSKRGWNYTAKSLKDIVDYTNENTLNGFLGHQKAENISTEFAPPVTHWIGA
                     EMKGDKAYFRGLIDADATNLKRWIRTKRIKEVSIFGYPKLKKSAKGEMNVIGYEPLSI
                     DWTPLHRPGMPTSIVGMEMSPNGEQLDGTFEALRIDLREALKAKFSINDNNSYLYIQN
                     IRYDNNTVIYELEQNGLCKLYSIPFTIVENKINLGEEIEVIKKISYEAKGEMKGEENK
                     LEGKELIKNVKGLLQTGEISYSEVIQGIGLTKEIVTGEMEDVKSSLKAEKELREVKKV
                     LGIVGEMDTVEVAKKASKALENEKKEAWNCIVNKVIKDKVSGEIAQTLVKKMLNVEEG
                     SSEEVITGEIENILNDEFVKNTMSNMYKDNPTTTGLLNSSNNGSLTTKKNRI"
     gene            5475..5858
                     /locus_tag="KR250_002773"
     CDS             5475..5858
                     /locus_tag="KR250_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2190 family protein"
                     /protein_id="HBH0068691.1"
                     /translation="MAFKGQPTPSTITQITRAKISDGKSVRVILSEGESTKTQQFYLI
                     NGFFGVAMQDGEKGDEVTLQIEQAEYETDNIVTSEAFEAGKLIYWDNTAKKFTTTSAS
                     NRLVGRVTDGKDSNNVIWFILLPQQ"
     gene            5877..6914
                     /locus_tag="KR250_002774"
     CDS             5877..6914
                     /locus_tag="KR250_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HBH0068692.1"
                     /translation="MAFKVISQENLLEQKRKETLQEDIPFIVNGEMEYVTKKISNGEM
                     ETLELNKPLGEMMTFSSTSNLKELLRKVVLDVELGREQVQLLYKPIYDSIADSNLPQV
                     MDAKWALQGNCVFLEHIEGEEIKFGTINAENGPVARIQTYATGFEYTKEMKDFNQTFS
                     VEILNKSIGESYNALLNHIHLSPIINFNYKASNKTAFKGETNDPIWLGIWRTLTQAQK
                     DTVIAKRQGNILMASSADQIEIEMALNGGHLLNGSMYPSIKNISTVIYYDGWEVTVGK
                     KTYSYKGVTPGKGYLIRPKRGFKELIKRDLTTEVGNADLSKLVENQIVGHCYRGAFAA
                     VEENVQEISFR"
     gene            6954..7292
                     /locus_tag="KR250_002775"
     CDS             6954..7292
                     /locus_tag="KR250_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0068693.1"
                     /translation="MTPTSNLIEKLRLLLNDKDKKSFTDEELNLFLEEADCIYCAASQ
                     GWVLKSLQYENTVGEMYEYKVGQETYKSSSIKDLVSVAYQNADKFKDMCTNKKEKGSF
                     MLGISTEFEI"
     gene            7289..7666
                     /locus_tag="KR250_002776"
     CDS             7289..7666
                     /locus_tag="KR250_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0068694.1"
                     /translation="MINIDRRRKDIIRTININPTNITITSIKKTEIDGAFEETETEIK
                     CVVRIFNEKTAEKQISSEKQGTFSSIRTYGMLVSDDVVLDVNSRDSLEFECIYGRMKI
                     VNVYPQIVKGELCGYQCSLERID"
     gene            7668..8078
                     /locus_tag="KR250_002777"
     CDS             7668..8078
                     /locus_tag="KR250_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0068695.1"
                     /translation="MSAFTNAINDINRKKAGMFVLCMSASAMLEGEAKANARWTDRTS
                     HARQSLNAKTLSGGNNFIIRLSHGAEYGGILEEGSKPHVITPKSAQALYWRGASHPVK
                     SVQHPGTKAMPIIKPTIDKNIGKIGNMIFRYWSD"
     gene            8079..8900
                     /locus_tag="KR250_002778"
     CDS             8079..8900
                     /locus_tag="KR250_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0068696.1"
                     /translation="MRAGIRKALIDNIKELKGCYEPNVPNKDTKKPYMVVVQGQDNDN
                     GETIGFERSIEVWIYEGRTTFKKLDKLTKQVIEILDMNTIVDESENEAFTCIYKGTSE
                     NDIVVEEWDAIARGIRFSVIALEDKEDTTNDRWVEALSRHTKDLLEIESYKDNWKKNF
                     IAPCALWRTTHIENKRINYHLIEITKTMKCHVVSKNKDEIVKLLETLETSLIIDKRVR
                     LREDKNMYLTLVSVVEDRESDMFTTGQLTAVFKMIGKIKREGPTMDKIYGNGNLR"
     gene            8913..9116
                     /locus_tag="KR250_002779"
     CDS             8913..9116
                     /locus_tag="KR250_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0068697.1"
                     /translation="MAETNNKKINVSKQEEKYLKSDFIENSEALGYKKEVVAGALFNC
                     KKEELTKSEFEKAIKEFLEREVK"
     gene            9118..10539
                     /locus_tag="KR250_002780"
     CDS             9118..10539
                     /locus_tag="KR250_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HBH0068698.1"
                     /translation="MATGTWNEKEKKEIPGFYNRFKTQAEKSTNTGLKGRLAMPIRAN
                     WGEVGKVVTIKNDLRQLKTLFGDDMNYSAFKLGKLALLGNVKELLLYRLVDGNQKKGT
                     LTLKDTTENSAKDVIKLETKYPTSRNFNVTIKSNLVDADKKDFIFFEGTKQLFSSSVK
                     GTIDEIVLEINSNLDNEYVIATKVADSDTALANLVNVALEGGNDGCTSITNESYLKAL
                     EEFERYSFDSFVLDGVAEEALQETTKAWVAKNKELGKDILLFLGGKAEDNIKQINDKS
                     KSFNDENIVNVGSSAYYEGIKYTPSEVAVYIAALSVSKGITGSICNAKTIFEEVEPRL
                     SQSEVKECLKSGTLILDFDDGDVIIVDDVNTFKKYVDDKNEAIGYISNIMFINTINKD
                     TSLKRKEFVGKIFNDSTGQTTVICALKKYFEELMGQGIISEFNVDIDTELQATAKADE
                     FYWKWDAVKVDVMKKIFGTGYLG"
     gene            10555..10977
                     /locus_tag="KR250_002781"
     CDS             10555..10977
                     /locus_tag="KR250_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0068699.1"
                     /translation="MGKYDENIIDAANVADGSNAKIIVDGKEEGYGTEFTAEVENDKK
                     TFRVIGCKWELNKASTQKGTFSLTVLKTTSEWIERGFDKFELISEIENPGLVGYERIR
                     YKNCMVDKIQLASIKADENIEIQIDGTFEGYELVDKIA"
     gene            11056..11529
                     /locus_tag="KR250_002782"
     CDS             11056..11529
                     /locus_tag="KR250_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0068700.1"
                     /translation="MANLDKEFLNEGIEEERELTKEEIAKQQEDNIIMKLTEDAILPE
                     KTIFVKRLDIPLTLRALTEKEISALQKKYTKVTKVRGRRESKLMEDEFNIALIEKATI
                     VPNFCDARLLNSMNVSSGVEFIRRKFLAGEIALISDEVLELSGFYEELSDDDIKN"
     gene            11710..16038
                     /locus_tag="KR250_002783"
     CDS             11710..16038
                     /locus_tag="KR250_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HBH0068701.1"
                     /translation="MREIAKKEMYHIDVVIDVTGDEQTKNKLSAMERYTKQTEKRMRA
                     LNRIKANPVIQAQDKTSSVVNRISNNLKRVGRTISTTINAKDRASSVVNRVKNKVNSL
                     LTSRQREVLLKARDKASQVVDKVKAKVQNLTAATIISLNMKADPALRVISQTRSKLGE
                     LKNNTIINIKAKGEEALNTISRTKSKLQEFSNRTYQAIVKLKDEASPTLSGLDGKISS
                     FISSTISKFTQLAVTATALIGGVGVGSAIKGFADFEQAMKNAQAVSSANSKEMAEMTA
                     MAREMGRTTSFTAKDAGNAMYFMGMAGWKSKEMIAGLPGILNLAATGQTDLALTADIV
                     TDGLTALGLTAKDTGMFVDVMAATVTNSNTDIERMGETFKYMGSVGGALGVSMKDLSL
                     ATGLMASASVKGSMAGTALRGGLVRLIKPPAEAQKAMNKYGIEIKKTKDGNLDLASTI
                     VDLREKLGGLEGVQKSAAISSIFGRTAMAGWAAVVNASEKDFKKLTTAINESEGEAKR
                     IADMKLDTLSGQFEILKSAIDDVRISVGQRLGPMTRSFVEQLTKDMPKIGDAIVSFVS
                     DFINDFDKIKSIMQNVISVISGIVSAFIAFKALKFISFLIPILSDIGFAIAAFAGGAA
                     TLGEALLFALGGPVGAVIAGVVLLATAFTLAYQKSDAFRKIVKNVGKSIKNFLQEAII
                     AISPFVNVFGNKLKELGKAVIPLLKVFGDFASTLMSKIGPIILFLSSNVLAGFILTFV
                     AAVEAVKSAVVAITGVLQGLTLIIKGVFDIVGGIISGDGKQIINGLKSVFEGGIKIVS
                     SVWKGLVDIVTSPIQAVVDVLDEKFGKKVEGIKKKWNELKDFLKNPTKAAPKVQPVNL
                     SGEKASNELQASSNGAKAYISSLGQKIGEGIGKIKDKFGELKISATEVFNNIVSFVGG
                     KATELKDKLLEGIKPAIDTFKQSLSNLKEVFGDSLDSIKEAFGGLKTVFDENIKTPFE
                     NLKQKVLETKESLKPVFDNLKSSFAELGKALEPIKEAFSGIKDFFSNLFKPIKDDGAT
                     KTTKTNMDELKQSTQRVGTSFKELGNAFNQLKEAAKPFIDYLKQIKDSLTSTLGDIGG
                     GLLKGVATSIVLVITSVINAIASIINAVAGAIKGVIDIIKGIFEIIGGIISGDGEKIK
                     QGFSDVFKGIGEVVKSLWEGIKGVLGAPLKAVVNFIDNGFSEKVGQVKQWWSDLKTNV
                     GQKISGFVSFVSNGFQQKVQQVGMWWQGLKVNLSGKISGFVSLVENGFKSKVDSIKSA
                     WDSLRKKLSQKITGFVSIVKTGISNVLDRFADGGVASKPSICGEAGPEMVIPLSNSKR
                     SRALSLYEQAGQMLGTKASNNVIPISQKLGTSFNSASSIQNSNSSIINNVRQFPTKQE
                     EFNNTENRIYQEAQPQNIISSGSNAINVGGISINIQGSNNKEEMIQEILSQVESGLRE
                     ALEDIG"
     gene            16118..16912
                     /locus_tag="KR250_002784"
     CDS             16118..16912
                     /locus_tag="KR250_002784"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0068702.1"
                     /translation="MWGRFKNMSIILKVLVLVCAIAIFPITLLAFSIEFVVKSFKRNE
                     RFKVVFGVFLVFITFSFVCIWYLLEDNITTSNSSNKKQEEHLKEEPGKNLATREAEEE
                     TNKKKEQEKQKNEGTEAEKKERDKTEKKEKVDKKYQEKNKEKAETKKQTLTSEELKKK
                     VDSIIPAEYKGNYYTNDVLDTDGSYVLSLQVQNASFDNESSCKAFTKDLINKLKEFRI
                     DSAEVYFVGSSGQTTYQINIDDFLKVQDNIKSVDNMEFFSFKDFKD"
     gene            16982..17653
                     /locus_tag="KR250_002785"
     CDS             16982..17653
                     /locus_tag="KR250_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBH0068703.1"
                     /translation="MVIDIYLKNEKEKIDFHFPVNPFDSLSIKKEKRFETVDIVNLGE
                     FDIKKEGEKIREISFKTFLPNLYDASYCRYSELKNPIEVVAMLEKWVDQAEPLRLIVT
                     GFGYNGLVTISNFSNTQTAGREEDRDIEITFRTYRELKIETLKKDTKSNTKTDLKDNR
                     PNTQTKSKIYTVKASDTLYKIAKNLLGKGSRWPEIYNIPENKKVIGKNPNIIKKGQKL
                     VIPSK"
     gene            17650..18612
                     /locus_tag="KR250_002786"
     CDS             17650..18612
                     /locus_tag="KR250_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdQ"
                     /protein_id="HBH0068704.1"
                     /translation="MKIILNGKYDIANFNEGITLSEAIDGVAYKMDVSLVEPKQLKDI
                     NIKKGDKIILIDIAYESKKEETIFDGVVWETRRSEKSKKLTLSCRERTVYMEESEEQY
                     QFKENTATQRIEYYCKQWNIPYYNLANTGKKLAKVIHKTNILDMIKKDLKETATKGGD
                     LFRVRMDNKLKLFKLGTNANVYKLDSILEDANFTSSFNDAVTSVKVLGKSKDENTKAP
                     IIGTYKKDADKFGTLQKIKQDEKIKNAKEAKKAAEAMFNSGEETISVDCAVDINRIRA
                     GDKVSLKSKEYYVIDVTHTLDSRPKMKLNIGSLEYIRRKFYNND"
     gene            18605..18970
                     /locus_tag="KR250_002787"
     CDS             18605..18970
                     /locus_tag="KR250_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0068705.1"
                     /translation="MTDARFNGIARILKENMNKSVANGTFGMGCELAEITANGLKVNG
                     YKDEIQDYLVLENLTLKEDYFTFSDEALSGEYRHKHKIETPKELKPLRIGDNVLVAVM
                     GAEFVVIGRVVNAKPISSK"
     gene            18945..19403
                     /locus_tag="KR250_002788"
     CDS             18945..19403
                     /locus_tag="KR250_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HBH0068706.1"
                     /translation="MPNLFPQSETFETVELKNNDENELDLKGSFLFDFEKGEFVKNAD
                     GTLKKCDKVQAYKQWCQKAILTPRYKKAAYTNIYGSEIKDLIASNLSQSAKELEITRL
                     IKETILVHPYTKEVSNFIFVWLENSRLVNYEFDVLTIDDENITIDGNIKR"
     gene            19412..20548
                     /locus_tag="KR250_002789"
     CDS             19412..20548
                     /locus_tag="KR250_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HBH0068707.1"
                     /translation="MERELPIPLFLTEEEDSVHERMLSNFQDVSTLEGDFIYDATRPT
                     AEQIAELKQLGLQNNLRIAFPQTSYGEYLEWLGECKGVFKNQPTKSVGMVTFNGAQGT
                     IITKGTIVTTIATDEKQSIEFELLETKTIGANETVDIKAECRIAGTIGNVSNNTITVL
                     LGSISGVKSVSNKEDFRGGTDIEDEEHFRERVLVAEQEDKLSGASSDYIRWAKEVDGV
                     GYAYVVPEWNGAGTVKVLILDKNRKAATQELIDKVQEYIYPLNISEGENRDGKAPIGA
                     LVTVVTPDTLLINVKASFIFSNGFSEETVLNNLKTKIDKYLDKIDLGGTVSYNAIQAI
                     VGSMMLTDEGIEDFSNLTINDVKENIKLQDQVVGIGEIVNEVVG"
     gene            20545..21174
                     /locus_tag="KR250_002790"
     CDS             20545..21174
                     /locus_tag="KR250_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HBH0068708.1"
                     /translation="MIASKKGKEMLLTLSPIYEQSIIMQSLYEAIGSEFDNLELLNKE
                     IELQLFPQSATWGLEFWENRVGLSTNIDEDIEARRRKVIAKLQSKYIMTPKRMAMILQ
                     SYTGANIKINENISPYTFGVELTSTQGFPKDLEDLYKRVNVIKPSHLAVSYKLVSLLK
                     SKTYFAQTAIMSEEITVYPYTSKEVKASVKAKFALAHNMSSETLTVYPR"
     gene            21187..>21842
                     /locus_tag="KR250_002791"
     CDS             21187..>21842
                     /locus_tag="KR250_002791"
                     /inference="COORDINATES: protein motif:HMM:NF023985.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBH0068709.1"
                     /translation="MADEQFYTILTNIGKAKIANAGMLGKSVILEKIQAGDGGGNYYN
                     PTEDQTALKNKVWEGNINAFDNDENNPNWIIATACIPGSIGGFTVREMGLIDNEGDMI
                     AVCKSPETYKPKVDNGAMKDLYLKFIIEVSNVEKVTLVVDPTAIFLTKKDEEKILTNI
                     NKLDTKIDTTKTELTSNIETTKTELNTRIDTENEKQNIKIDQLIAGGSNVASTQIITI
                     "
BASE COUNT         8985 a         2394 c         4321 g         6142 t
ORIGIN      
        1 gctatgagtg agttaaggag tttaattaaa cagtatgatg aaatgattca taaggattgg
       61 aatttagcta cagaggagca gaaaaataga gttgaaaagt taaaatgtga agttgataac
      121 ctaaagaaaa gtgatactgg agatgattca aaaatttggg ttgaagctat acaaaatatt
      181 gcaatgaaac gtggtgttaa caatggataa agctttattg acactattag attgttattg
      241 ggataatcct gtttggtttg cagaggatat gttaaatttt aaagctgaca agtggcaatc
      301 tgatgttctg atggctttag ctcaaacccc aaaagtatct attagaagtg gtcaaggagt
      361 aggtaaaact ggattagaaa gcattgcaac tgtatggtat ttaagcacta gaccttttcc
      421 gaaagtagtt gctacagctc caacacgaca acaattatat gacgtactat gggctgaaat
      481 agctaaatgg ctaagtaata gcaaggttga gaagctactt gagtggacta aaacaaaagt
      541 gtatatgaaa ggctttgaag aaagatggtg ggctacagct agaacagcag taaagcccga
      601 gaatatgcaa ggttttcatg aagattatat gttatttgtt gttgatgaag cttcgggagt
      661 tgctgacccc attatggaag ctatattggg aacattatca ggtgcagaaa ataagcttct
      721 tttatgcgga aacccaacta gaacgagtgg aacgttttac gatagccata atagagacag
      781 agatttatat aaaacattta aagtatcttc tttagatagc cctagaacat caaaagataa
      841 tattgaaatg ctaaaaagaa agtaccatga aggttctgac ccttggcgtg ttagagttct
      901 tggagaattt ccaaaaggtg aaagtgattc tttaatatct ttagaagctg ttgaaacaag
      961 cacaataaga gaagtgaata tatctaatga ctatatatta aatataggtg cagatatagc
     1021 aagatatggt gacgatgaaa ctataatagc tccacgaata ggtggaaaag tgtttgattt
     1081 attaacttat tctaaaaaag acacaatgga aactgtagga aatatattaa gagcagttga
     1141 taaatttaaa agcatgtatc atcaaatcaa cagagtaaaa ataaaaacgg atgatgatgg
     1201 tttgggtgca ggtgttacag acagattaaa agaggttata agacaagaaa gactcaaata
     1261 tgaaattgta cctattcaaa atggttctag tgctatagaa aaagataaat actataataa
     1321 agcttctgaa atgtgggata acatgaggga ggaattagat gcaaatttaa gtggcttcat
     1381 acaaaataaa gaagctataa tacagcttcc taatgatgat aaacttatta agcaactatc
     1441 aaatagaaaa tatacagtag attcaaaagg gaaaatacaa atagaaagta aaaaggaaat
     1501 gaaaaaaaga attggagaat cacccgatag agctgatgca gtaatatatt cgtttgcaga
     1561 aaataacaat actgatttat ctttactgaa agggggtagt gtatggggat aatatcttat
     1621 gtaaaaaagc tatttaaaag acctactgga gaaattatgc gtatgtctag tggaaatata
     1681 ggcgtatata aattagatga ttctagagtt gattatgagt tagcaagaga attgtatcaa
     1741 aacaaaaatg ctaattataa attaggttct agttttgtta gaccaattgt caattcaaca
     1801 actggtttta tgggtgtacc tcattttcaa atagaagatg aagaagctca atatatatta
     1861 gatgaatttg ttttagataa cacatctaaa atgttaaaaa cacacacaga tagtttaaag
     1921 caaggtgatt gttatatttg gataactaga gaagaaagag aaaatccttt atatcctgat
     1981 aaaaaagtta gattaatata taacttcata tcacccgaag aagtgaaaga aataatatta
     2041 gaccctacga caaaagagcc tatagcttat atattagaaa gccaaaatga atggactgac
     2101 ttagaagaaa ataagagaag ggctaaggta aaacaaataa taactgctga aagtagaatt
     2161 attgaggttg aaggggataa gatagaaggt ttagaagaag gggaaactcc taatgtatgg
     2221 ggctttatac caataataca ttttaaaaat gaagctgatg aaacattgaa atatggtcaa
     2281 agtgatatag aaccaataga acctctttta aaagcttatc atgatgttat gttacatgcg
     2341 ttaaaaggta gcaaaatgca ctctactcca aaactaaagc tgaaattaac tgatgttgca
     2401 agtttcttag cacacaactt tggtgttgaa gacccagtta aatttgcaaa agagggtgga
     2461 aagataaatc ttgatggaca tgaaatacta ttcttaaata aagatgaaga agctgagttt
     2521 gtagaagtaa aatcagctat aggtgatgct aaggagcttt taaagcttct tttttattgc
     2581 atagtagatg tatctgaaac acccgagttt atatttggag tacatacacc tagtgcttta
     2641 gcttctgtaa aagaacaaat gcctattatg gtaaataaga taagaagaaa aagagaacaa
     2701 tttacaaata gctggcaatt acttgcaaga atggttttaa taatgagttc taattctagt
     2761 ggtatgaaat attcatctta tgatgtgact ataggttggg atgaagtaaa tccgcgtgat
     2821 gataaagaat tagctgaaac actagaaaag gtatgtagtg cattagataa agctttagaa
     2881 ggtggattta ttagcgaaga atcaacagta aactttttag ctcagtatat agatacaatg
     2941 agcaattata taagtgatga ccctgaaata gttggagaaa gagaaaagat aataaaaact
     3001 aaaatgttaa aatacagatt agatgattct caaggtttag atgatgagtc aaatgaaatt
     3061 gacaaggaaa tagataaaat aaaggataat aatgacaatg gataaaagta ctttggaatt
     3121 aataactgtt gcaggggagt acaagaaatg ggcattagaa gctagaaaaa aatttataga
     3181 tttaagactc aagcaagatg atgaaataag aacaatgtat attaacataa caagaaatat
     3241 tacaaaagaa ataagaaaag gaaatctttc agactttaac aaagttaggt taaaacagat
     3301 actaaaacaa ttaacacaag aaataaaaat attaaatgaa caactagtat ttaattttga
     3361 tgaatactta aataaaaatg ttgaaacagc tactagttac tctaaaaata ttttaattaa
     3421 tgcagttgag acagctcaaa taactaaagt aactaaagct atgatacaaa aagctttcta
     3481 tgatattaat ataagaactg tagaagctta ttatacaagg gttaaggatg gtttattttt
     3541 atctgataga atttggtcta agtgtaagaa gtacagagaa gatatgaaag ttatattaca
     3601 aacagcagta acagaaggtc aagactgtgt taaaacagct aagatgctag acaaatacgt
     3661 tttaaaaggt aagaaaactt tagttgacga atatccaaat atgataaaaa gaataggaaa
     3721 tagagtacct caaaatataa gttatgaagc tttaagattg gcaagaactg aaatgacatc
     3781 agcttatggt gatggggtct tagcttctgc aatgattaac cctgcaacca taggtattca
     3841 gtttatgttg tccatggcac atcctcatac agatatatgc gacgaaatat gtggagagga
     3901 taattttggt ttgggtaaag gtgtttatcc tataaatgaa gctcctgcat atccattcca
     3961 ccctcattgt ttgtgtatta tgcttactgt agttcaacca ttagatatat tagttggaag
     4021 gttgaaaaat tggattaaaa atcctatgaa tgatacacct cttgaaatgt ggtatcaaga
     4081 ggtgtatgga aatttgaatt tttaaattga aaggtggtga ttaaatgaat gtaataactg
     4141 gagaaatgga ctcaatgaat gcgttaatat ctagtataaa accttctgat attcctttag
     4201 ctaaagatat agacatagaa gctttaaaat ctatagatga tgaccctctt gaggtagttg
     4261 ttgagatacc agctacaaaa tctaaaaggg gatggaacta tactgctaaa agcttgaaag
     4321 atattgtaga ttacactaat gaaaatactc ttaatggttt tttaggacat caaaaagctg
     4381 aaaatatatc aactgaattt gcaccacctg taacgcattg gataggtgca gaaatgaaag
     4441 gggataaagc ttatttcaga ggactgattg atgctgatgc aacaaacttg aaaagatgga
     4501 ttagaactaa aaggataaaa gaagttagta tatttggtta tccaaaactt aaaaagagtg
     4561 ctaaaggcga aatgaatgtt ataggatatg agccactatc tattgattgg actcctctac
     4621 atagaccagg tatgccaaca agtattgtag gtatggaaat gagtcctaat ggcgaacagt
     4681 tagatggaac ttttgaagct ttaagaatag atttaagaga agctttaaaa gctaagtttt
     4741 ctattaatga taataattca tatctctata tacaaaacat aagatatgat aacaatactg
     4801 tcatatatga gttggagcaa aatggattat gcaagcttta tagtatacca tttactatag
     4861 ttgaaaataa aataaatcta ggtgaagaaa ttgaagtaat aaagaaaata agctatgaag
     4921 ctaaaggaga aatgaaagga gaggaaaaca aattggaagg aaaagagtta ataaaaaatg
     4981 tcaaaggatt actgcaaact ggtgaaatat catattcaga ggtcatacaa ggaataggct
     5041 taactaagga aattgtgaca ggagagatgg aagatgtaaa aagttcatta aaagcagaaa
     5101 aagaattaag agaagtgaaa aaagtacttg gaatagtagg agagatggac acagttgaag
     5161 tggcaaaaaa ggcttcaaaa gctttagaaa atgagaaaaa ggaagcttgg aactgtatag
     5221 ttaataaagt aattaaagat aaagtgtcag gtgaaatagc tcaaacatta gttaagaaaa
     5281 tgttaaatgt tgaggaaggc tcaagtgaag aagtaataac aggagaaata gaaaatatat
     5341 taaatgatga gtttgtaaaa aatacaatgt ctaatatgta taaagataat ccaacaacaa
     5401 caggattatt aaactctagc aataatggaa gtttaacaac taagaaaaat agaatataaa
     5461 ggagtgatgt ttatatggca tttaaaggtc aaccaacgcc aagcacaata acacagataa
     5521 caagagcaaa aataagtgat gggaaatctg taagagttat tctttcagaa ggtgaaagca
     5581 ctaaaacaca acaattttat cttataaatg gattctttgg agtcgctatg caagacggag
     5641 aaaaaggcga tgaagttact ttgcaaatag agcaagctga atacgaaacg gataatattg
     5701 ttacatcaga agcttttgag gcagggaaat tgatttattg ggataataca gctaagaaat
     5761 ttactactac atctgcaagt aataggctag ttggtagagt aacagatggg aaagacagta
     5821 ataatgtaat ttggtttata ttattacctc aacaatagaa aaggagtgat aaatatatgg
     5881 catttaaagt aattagtcag gaaaatttgc tggaacaaaa aagaaaagaa actttacaag
     5941 aagatatacc atttatagta aatggtgaaa tggaatatgt aacaaagaaa atatcaaatg
     6001 gagaaatgga aaccttggag ttaaataagc cacttggtga aatgatgact tttagctcga
     6061 cttcaaattt aaaagagtta ttaagaaaag ttgtattaga tgttgaacta ggcagagagc
     6121 aagtacaact attatataaa ccaatctatg acagtatagc agattctaat ttaccacaag
     6181 ttatggatgc taagtgggct ttacaaggta actgtgtatt cctagagcat atagaaggtg
     6241 aagaaattaa attcggtaca ataaatgcag aaaatggtcc agttgcaagg atacaaactt
     6301 atgcaactgg ttttgagtat acaaaagaaa tgaaggattt taaccaaaca tttagtgttg
     6361 aaatattaaa taaatcaatt ggtgagagtt acaatgcctt gttaaaccac atacatctaa
     6421 gcccaataat aaattttaat tataaagctt ctaataagac agcttttaaa ggtgaaacta
     6481 atgacccaat atggctagga atttggagaa cattaacaca agcacaaaaa gatacagtta
     6541 tagcaaaaag acaaggtaat atattaatgg cttctagtgc tgaccaaatt gaaatagaaa
     6601 tggcgttaaa tggaggacat ttattaaacg gaagcatgta tccatctata aaaaatatat
     6661 caacagtaat ttattatgat gggtgggagg ttactgttgg taaaaaaaca tattcttaca
     6721 aaggtgttac accaggcaaa ggatatttga taagacctaa gcgaggattt aaagagttaa
     6781 taaagagaga tttaacaaca gaggttggaa atgctgattt aagtaagtta gtagaaaatc
     6841 aaattgtagg tcattgttat agaggtgctt ttgcagcagt agaagaaaat gtacaagaaa
     6901 taagttttag ataaaacact cataagagtg ttatttttat gaggtgataa tatatgacac
     6961 caactagtaa tttaatagaa aaattaagac tattattaaa tgataaagat aaaaaatcat
     7021 ttacagatga agaattaaac ttgtttttag aagaagcaga ctgtatttac tgtgcagctt
     7081 ctcaaggatg ggtattaaaa tctttacaat atgaaaatac agtaggggaa atgtatgagt
     7141 ataaagtggg tcaagaaaca tataaaagct ctagtataaa ggacctagta tctgtagctt
     7201 atcaaaatgc agataaattt aaggatatgt gtactaacaa aaaagaaaag ggaagtttta
     7261 tgttaggaat tagcacagaa tttgaaatat gataaatatt gatagaagaa gaaaagatat
     7321 aataagaact attaatataa accccactaa tattactata actagtatta aaaaaaccga
     7381 aatagatgga gcttttgaag aaactgaaac agaaataaaa tgtgttgtta gaatatttaa
     7441 cgaaaagaca gcagagaagc aaatatcaag tgaaaagcaa ggtacattta gttctattag
     7501 aacatatgga atgttagtaa gtgatgatgt tgtcttagat gttaacagta gagattcttt
     7561 ggagtttgag tgcatatatg ggagaatgaa aatagttaat gtatatcctc aaattgtaaa
     7621 aggagaactt tgtgggtatc aatgttcact tgaaaggatt gattaaaatg agtgctttca
     7681 caaatgcaat aaatgatata aatagaaaaa aagcaggtat gtttgtactt tgtatgagtg
     7741 caagtgcaat gctagaaggt gaagctaaag caaatgcacg ttggacagat agaacatcac
     7801 atgcaagaca aagtttaaat gctaaaacac ttagtggagg aaataatttt atcattagat
     7861 tatctcatgg tgcagaatat ggagggatac ttgaagaagg ctcaaaacca catgttatta
     7921 ctccaaaatc agctcaagcc ctatactgga gaggtgcttc acatcctgta aaatcagttc
     7981 aacatcctgg tacaaaagca atgcctatta taaaaccaac tattgataaa aatataggca
     8041 aaataggtaa tatgattttt agatattgga gtgattaaat gagggcagga ataagaaaag
     8101 ctttaataga taatataaaa gaattgaaag gttgttatga acctaatgta ccaaacaaag
     8161 atactaaaaa gccttatatg gtagttgtac aagggcaaga caatgacaat ggagaaacga
     8221 taggttttga aagaagtata gaagtatgga tttatgaagg tagaactaca tttaagaagt
     8281 tagataaatt aactaaacaa gttattgaaa tcttagacat gaatactata gttgatgaat
     8341 ctgaaaatga agcttttact tgcatttata aaggtacaag tgaaaatgat attgttgttg
     8401 aggaatggga tgctatagca agaggtataa ggtttagtgt aatagcttta gaagataaag
     8461 aagatacaac taatgatagg tgggtagaag ctctatctag gcacacaaag gatttattag
     8521 aaatagagag ttataaagat aattggaaga aaaactttat agctccatgt gcattatggc
     8581 gaactacaca tattgaaaat aaaagaatta actatcattt aattgagatt actaaaacta
     8641 tgaaatgtca tgttgtaagt aaaaataaag atgaaatagt taagcttctt gaaacattag
     8701 aaacaagctt aataatagat aaaagagtaa gacttagaga agataagaat atgtatttaa
     8761 ctcttgttag cgtagttgag gatagggaat cagatatgtt tacaactgga caattaacag
     8821 ctgtgtttaa aatgatagga aagataaaaa gagaaggtcc tactatggac aaaatttatg
     8881 gtaatggaaa tttaagatag gaggtgtaag ggttggctga aacaaataat aaaaagatta
     8941 atgtgagtaa gcaggaagaa aaatatttaa aaagtgattt tatagaaaat agcgaagcac
     9001 ttggctacaa gaaagaagta gttgcaggtg ctttatttaa ttgtaagaaa gaagaactta
     9061 caaagtcaga gtttgagaaa gcaataaaag agtttttaga aagagaggtg aagtaaaatg
     9121 gcaactggta catggaatga aaaagaaaaa aaggagatac ctggttttta caatcgcttt
     9181 aaaacacaag cagaaaagtc tacaaacaca ggattaaagg gtagattagc aatgcctatt
     9241 agggctaatt ggggagaagt tggcaaggtt gtaacaataa aaaatgattt gagacaactt
     9301 aaaactctat ttggtgatga tatgaattat tcagctttca agctaggaaa actagcttta
     9361 ctaggtaatg taaaggaatt acttttatat aggctcgtag atgggaacca aaaaaaaggt
     9421 acattaacac taaaagatac tacagagaat agtgcaaaag atgtaattaa gctagaaact
     9481 aagtatccaa ctagcagaaa ttttaatgta acaataaaat ctaatctagt agatgcagat
     9541 aaaaaagact ttatattctt tgaaggaact aaacaattat ttagttcaag tgtcaaaggg
     9601 actatagatg aaatagtact agaaataaac tcaaatttag ataatgagta tgtaattgca
     9661 actaaagtag ctgatagtga tacagcacta gcaaacttgg taaatgtagc acttgaaggt
     9721 ggtaacgatg gttgcacatc tattactaat gagtcttatc taaaagcact agaagaattt
     9781 gaaagatata gttttgactc ttttgtactt gatggtgtgg ctgaggaagc attgcaggaa
     9841 actacaaaag cttgggtagc taagaataag gagctaggaa aagatatatt attatttctt
     9901 ggtggaaaag cagaggataa tataaaacaa attaatgata aatcaaaaag ttttaatgat
     9961 gaaaatatag ttaatgttgg gagttcagct tattatgagg gaataaaata tacacctagt
    10021 gaagtagctg tttatattgc tgctctttct gtaagtaaag gtataacagg aagtatatgt
    10081 aatgctaaga ctatatttga agaagtagaa ccaagattaa gccaaagtga agtaaaagag
    10141 tgtttgaaat caggtacact aatacttgat tttgatgatg gagacgtaat tatagttgat
    10201 gatgtaaaca catttaagaa gtatgtagat gataagaatg aagctatagg atatatctct
    10261 aatattatgt ttattaatac tataaataaa gatacttcat tgaagcgaaa ggaatttgta
    10321 ggtaagatat ttaatgattc aacagggcag acaactgtta tatgtgcatt aaagaaatat
    10381 tttgaagaat taatgggtca aggcattata tcagaattta atgttgatat agatacagag
    10441 cttcaagcaa ctgctaaagc agatgagttt tattggaaat gggatgctgt caaggttgat
    10501 gtgatgaaga aaatctttgg aactggatat ttaggataaa ggaggttata aagtatggga
    10561 aaatatgatg aaaatattat agatgctgca aatgttgcag atggctcgaa tgctaaaata
    10621 atagttgatg ggaaagaaga aggatatgga acagagttta cagctgaggt tgaaaatgat
    10681 aagaaaacct ttagagtgat tggttgcaaa tgggagctta acaaggcatc tactcaaaaa
    10741 ggtacttttt ctttaactgt acttaaaaca acatctgaat ggatcgaaag aggatttgat
    10801 aaatttgaat taatatctga aatagaaaat cctggattag ttggatatga aagaattaga
    10861 tataaaaatt gtatggtaga taaaatacaa ctagcaagta taaaagctga tgaaaatata
    10921 gaaatacaaa tagatggtac ttttgagggt tatgagctag tggataaaat agcataataa
    10981 ataaatttaa gctacatgta attaatttta tatgtagctt ttcaaataaa actataaaaa
    11041 tggaggaagt taaaaatggc aaacttagat aaagaatttt taaatgaagg aatagaagaa
    11101 gaaagagaac ttactaaaga ggaaatagca aagcaacaag aagataatat aattatgaaa
    11161 ttgacagagg atgctatatt acctgaaaaa actatttttg taaaaagatt agatatacca
    11221 cttacactta gggctttaac agaaaaagag ataagtgcgt tgcaaaaaaa atatacaaaa
    11281 gttactaagg taagaggtag aagggaaagc aaactaatgg aagatgaatt taatatagct
    11341 ctaatagaaa aagctacaat agttcctaat ttttgcgatg caagacttct taattctatg
    11401 aatgtatcta gtggtgttga atttataaga agaaagttct tagcaggaga aatcgcatta
    11461 attagtgatg aggtactaga attgtctgga ttttatgaag aattaagtga tgatgatata
    11521 aaaaactaat aaagagaggt gggaagatta ctattttata caacgcatat gttaaacata
    11581 gtattcttcc agaggacttt ttaaaaagag agaaaacacc tcaacagctt cttagagttt
    11641 ttactcaaca tgaaatagaa caagaaaata aagctatgaa aaataaataa aatttaaact
    11701 aaaagtgagg tgagagaaat agctaaaaaa gaaatgtatc acattgatgt tgtcattgat
    11761 gttacaggag atgaacaaac taaaaataaa ttaagtgcta tggaaagata cacgaaacag
    11821 acagaaaaga gaatgagagc actaaatagg ataaaagcta atccagttat acaagctcaa
    11881 gataaaacat ctagtgttgt aaatagaatt agcaacaact taaaaagagt gggtagaact
    11941 atatctacaa ccataaacgc aaaagataga gcatctagcg ttgtaaatag agttaaaaac
    12001 aaagtaaata gcttacttac aagtcgacaa agagaagttt tattaaaggc tagagacaaa
    12061 gctagtcaag ttgtagataa agtaaaagct aaggtacaaa atttgactgc ggctacaata
    12121 attagcttga atatgaaagc tgacccagca ctaagagtta tttctcaaac tagaagtaag
    12181 ttaggagaac tcaaaaacaa cacaataata aatattaaag caaaaggtga agaagcatta
    12241 aatactattt ctcgtactaa gagtaaatta caagagtttt ctaataggac ttatcaagca
    12301 attgtaaaat taaaagatga agctagtcca actttgagtg ggttagatgg taagataagt
    12361 tcttttataa gtagtactat tagtaagttt acacaattag cagtaacagc cacagcatta
    12421 attggtggtg ttggagtagg aagtgctata aaaggatttg cagactttga acaagcaatg
    12481 aaaaacgcac aagctgtatc aagtgcaaat tcaaaggaaa tggcagaaat gactgcaatg
    12541 gcaagggaaa tgggtcgtac aactagcttt acagctaaag atgcagggaa tgctatgtat
    12601 tttatgggga tggctggatg gaaaagcaaa gaaatgattg ctggtctacc tggtatatta
    12661 aatttggcag caacaggtca gacagattta gcactaacag cagatattgt gactgatggt
    12721 ttaactgctt tagggctaac tgcaaaggat acaggaatgt ttgttgatgt tatggcagca
    12781 acagttacaa actctaacac agacatagaa agaatgggtg aaacttttaa gtatatggga
    12841 agtgttggag gagcattagg tgtttctatg aaagatttga gtttagcaac tggcttaatg
    12901 gctagtgcaa gcgttaaagg tagcatggca ggtactgcac ttagaggtgg tttagttaga
    12961 ttaataaaac ccccagctga ggcacagaaa gctatgaata aatatggaat agaaataaag
    13021 aaaacaaaag atggaaattt agatttagct agtacaatcg ttgaccttag agaaaaatta
    13081 ggcggacttg aaggagtaca aaaaagtgct gcaataagta gcatatttgg gcgaacagcc
    13141 atggccggtt gggcggctgt agtaaacgca agtgaaaaag attttaaaaa attaacaaca
    13201 gctataaatg aaagtgaagg agaagctaag agaattgctg atatgaaact agataccctg
    13261 tcaggacaat ttgaaatttt aaaaagtgct attgatgatg taagaatatc agtaggtcaa
    13321 agacttggac ctatgacacg aagttttgtt gaacagttaa caaaagatat gcctaaaata
    13381 ggggatgcca tcgttagttt tgtaagtgat tttataaatg attttgataa aattaaaagt
    13441 attatgcaaa atgtcatttc agtaatatct ggaatagtat ctgcatttat tgctttcaaa
    13501 gctttaaagt ttatttcttt tctaattcct atattaagcg atataggatt tgcaatagct
    13561 gcttttgcag gtggtgcggc aacgctagga gaagctttat tgtttgcact aggtggacct
    13621 gttggtgctg ttatagcagg agtagtatta ctagcaacag catttacatt agcttatcaa
    13681 aaatcagatg cttttagaaa aattgtcaaa aatgtaggaa aatcaattaa aaatttttta
    13741 caagaagcaa taatagcaat ttcacctttt gtaaatgtgt ttggtaacaa attaaaagaa
    13801 ttagggaaag ctgtaattcc attgttaaaa gtatttgggg attttgcatc aacgctaatg
    13861 agtaaaatcg gacctataat tttgttttta tcaagtaatg ttttagctgg ctttatatta
    13921 acttttgtag ctgctgtaga agctgttaaa tctgctgtag tcgcgataac aggtgtattg
    13981 caaggtttaa cattaattat aaaaggagtt tttgatattg ttggaggaat aataagtggt
    14041 gatgggaaac aaataataaa tggcttaaaa tctgtatttg aaggaggaat aaaaattgtt
    14101 tcttctgttt ggaaaggatt agtagatatt gtaacttctc ctattcaagc tgttgtagat
    14161 gttctagacg aaaaattcgg aaaaaaagta gagggaataa agaaaaaatg gaatgaatta
    14221 aaagacttct taaaaaatcc tactaaagca gctccaaaag ttcaacctgt caatttatct
    14281 ggagagaaag catcaaacga gttacaagct tcatcaaacg gagcaaaagc atatataagt
    14341 tcgttaggtc aaaaaatagg tgaaggtatt ggaaaaatta aagataaatt tggagaactc
    14401 aaaatatctg caacagaagt atttaataat atagtatctt tcgtaggagg caaagcaacc
    14461 gaattaaaag ataaactttt agaaggcata aaacctgcta tagatacatt taaacaatca
    14521 ttaagtaatt taaaagaagt ttttggagat tctttagata gcataaaaga agcttttgga
    14581 ggtttaaaaa ctgtatttga tgaaaatatt aaaacgcctt tcgaaaattt aaaacaaaaa
    14641 gttttagaaa caaaagaaag tttaaaacca gtttttgata acttaaaatc tagctttgca
    14701 gaactaggaa aagctcttga accaataaaa gaagcattta gtggaataaa agatttcttt
    14761 tcaaatttgt ttaagccaat taaagatgat ggagcaacta agacaactaa aaccaatatg
    14821 gatgagttaa aacaatcaac acaacgtgtt ggaacatctt tcaaagagtt aggaaatgct
    14881 ttcaaccaat taaaagaagc agcaaaacct tttatagact atttaaaaca gataaaagat
    14941 tctttaacat ctactctggg agatataggg ggaggattac tcaaaggtgt agcaacttct
    15001 atagttttag ttataacttc tgttattaat gcaattgcat ctattataaa tgctgtagca
    15061 ggtgctataa aaggtgtaat tgatataata aaaggaatat tcgaaatcat aggtgggata
    15121 attagtggtg atggtgaaaa aataaaacaa ggattttctg atgttttcaa aggaattggg
    15181 gaagtagtca aatcattgtg ggaaggtata aaaggagttt taggagcacc acttaaagca
    15241 gttgtaaatt ttattgataa tggattttca gaaaaagtag ggcaagtaaa acaatggtgg
    15301 tctgatttaa aaactaatgt aggccaaaaa ataagcggat ttgttagttt tgtaagcaat
    15361 ggttttcaac aaaaagttca acaagttgga atgtggtggc aaggacttaa agtaaattta
    15421 tctggcaaaa taagtggatt tgtaagtctt gtagaaaacg gatttaaaag taaagtggat
    15481 tcaattaaat cagcatggga tagtttaaga aaaaaattat cacaaaaaat aactggtttt
    15541 gtaagtatag taaaaactgg aataagtaat gtattggacc gttttgctga tggtggagtt
    15601 gcaagtaaac caagtatttg tggagaagca ggtcctgaaa tggttattcc tctttctaat
    15661 agtaagagaa gtagagcatt aagtttatat gaacaagcag gacagatgct tggaactaaa
    15721 gcaagcaata atgttattcc aatatctcaa aaattaggaa ctagttttaa ttctgcaagt
    15781 agtatccaaa atagtaattc tagtattatt aataatgtta gacaatttcc taccaaacaa
    15841 gaagaattta ataatacaga aaatagaatt tatcaagaag ctcaaccaca aaacataatt
    15901 tctagtggaa gtaatgcgat taatgttggt ggaatatcta taaatattca aggtagcaac
    15961 aacaaagaag aaatgataca agaaatattg tctcaagtgg aaagtggatt aagagaagca
    16021 ttagaagaca ttggataatg tcgaattgtt gttaaaaaaa tcctccttat agatgttata
    16081 atttaattat aaattacatg agggggattt gcatattatg tggggaagat ttaaaaatat
    16141 gagtataatc ttaaaggttt tagttttagt ttgtgctata gcaatttttc caataacttt
    16201 attagcattt tctatagaat ttgtagttaa atcttttaaa agaaacgaaa ggtttaaagt
    16261 tgtttttggc gtatttttag tttttattac attttcattt gtatgtattt ggtatttgtt
    16321 agaagataat ataacaacta gcaatagtag taataaaaaa caagaagaac atctaaaaga
    16381 agagccaggt aaaaatctag caacaagaga agcagaagaa gaaacaaata agaagaaaga
    16441 acaagagaaa cagaaaaatg aaggtacaga agctgaaaaa aaagagcgag ataaaactga
    16501 gaaaaaagaa aaagtagata aaaaatatca agagaagaat aaagagaaag cagaaacgaa
    16561 aaagcaaaca ttaactagtg aagaattaaa gaaaaaagtt gattctataa ttccagcaga
    16621 gtataaagga aattattaca caaatgatgt attagataca gatggtagct atgtacttag
    16681 tttacaagtt caaaatgcaa gttttgacaa tgaaagcagt tgtaaggcat ttactaaaga
    16741 cttaattaac aagttgaaag aatttagaat agactcagca gaagtatatt ttgtaggttc
    16801 aagtggtcaa acaacatatc aaattaatat agatgatttc ttgaaagttc aagataatat
    16861 taagagtgta gataatatgg agtttttctc tttcaaagat tttaaagatt agatatataa
    16921 gaaaaagacg tttatttatt aggaatagat gtctttttta ttgtaaaaag gaagtgataa
    16981 tttggtaata gacatatacc taaaaaatga aaaagaaaaa atagattttc attttccagt
    17041 aaatccattc gatagtttat ctattaaaaa agaaaaaagg tttgaaactg tagatatagt
    17101 aaacttaggc gaatttgaca ttaaaaaaga aggagagaag ataagagaaa tatcatttaa
    17161 aacatttctg cctaacttat atgacgcttc ttattgcaga tacagtgagt taaaaaatcc
    17221 aatcgaagta gtggcaatgc ttgaaaagtg ggtagaccaa gctgaaccat tacgattaat
    17281 agtaactggt tttggctaca atggattagt tacaatatct aattttagta atactcaaac
    17341 agcaggaaga gaagaagaca gagatattga gataacattt agaacttaca gagaactgaa
    17401 gatagagaca ttaaaaaaag atacaaaaag taatactaaa acagatttaa aagataatag
    17461 acctaatacc caaactaaat ctaaaatata tactgttaaa gcaagtgata cattatataa
    17521 gatagctaaa aatcttttag gtaagggttc aaggtggcca gagatttata atatacccga
    17581 aaacaaaaaa gtcattggta aaaatcctaa tataattaaa aaaggtcaaa agttggtgat
    17641 acctagtaaa tgaaaataat attaaacggg aaatatgata ttgcaaattt caatgaagga
    17701 ataactcttt cagaagctat agacggagtt gcatataaga tggatgtatc attagtagaa
    17761 cctaaacaac ttaaagatat aaatattaaa aaaggtgata aaataattct aattgatata
    17821 gcatatgaga gtaaaaaaga agagacaata tttgatggag ttgtatggga aactagaagg
    17881 agtgaaaaga gtaagaaact aacattgtct tgcagagaaa gaacagttta catggaagaa
    17941 tcagaggaac aatatcagtt taaagaaaat acagcaacac agagaattga atattactgt
    18001 aagcaatgga atatacccta ttacaactta gctaatacag ggaagaaact tgctaaagta
    18061 atacataaga caaatatact agatatgata aaaaaggact taaaagaaac agcaacaaaa
    18121 ggtggagact tatttagagt aaggatggat aataaattaa aattattcaa gcttggtact
    18181 aatgcaaatg tatataaatt agatagtata ttagaagatg ctaactttac aagtagtttt
    18241 aatgatgcag taacaagtgt aaaagtttta ggtaagagta aagacgaaaa tacaaaagcg
    18301 cctataattg ggacatataa aaaagatgct gataagtttg gaacactaca aaagattaaa
    18361 caagatgaaa agataaaaaa tgctaaagaa gctaagaaag cagcagaagc aatgttcaat
    18421 agtggagagg aaacaataag tgtagattgt gcagtagata taaatagaat aagagcaggt
    18481 gacaaagtaa gtttaaaaag taaagaatat tatgttatag atgtcactca tacactagat
    18541 tctagaccga aaatgaagct gaatataggg tctttagaat atataaggag gaaattttat
    18601 aataatgact gatgctagat ttaatggaat tgctagaata ttgaaagaaa atatgaataa
    18661 aagtgtagca aatggcactt ttggaatggg ttgtgaactt gcagaaataa cagcaaatgg
    18721 attaaaagta aatggctata aagatgaaat acaggactat ctagtattag agaatttaac
    18781 attaaaagaa gattatttta ctttttcaga tgaagcttta agtggagaat atagacataa
    18841 gcataaaata gaaactccaa aggaattgaa gccactacgt ataggcgata atgtgctagt
    18901 agctgttatg ggagctgaat ttgtagtaat tgggagggtt gtaaatgcca aacctatttc
    18961 ctcaaagtga aacttttgaa actgtagaat taaaaaataa tgatgaaaat gaattggacc
    19021 taaagggttc ttttttattt gattttgaaa aaggtgaatt tgttaaaaac gcagatggaa
    19081 cactaaaaaa atgtgataag gtgcaggcgt acaaacaatg gtgtcaaaag gctatattaa
    19141 cacctagata caaaaaagca gcttatacaa acatttatgg aagtgaaata aaagacttaa
    19201 tagctagtaa cttatctcaa agtgcaaaag agcttgaaat aactagatta ataaaagaaa
    19261 ctattttggt tcatccatat acaaaagaag ttagtaattt tatatttgtt tggcttgaaa
    19321 atagtagact tgttaattat gaatttgatg tgttaacaat agatgatgaa aatataacca
    19381 tagatggaaa cataaaaagg taggtgatta tatggaaaga gagctaccta taccattatt
    19441 tttaacagaa gaggaggact ctgtacatga aaggatgtta agtaactttc aagatgtgag
    19501 cacactagaa ggggacttta tttatgatgc aacaagacct acagcagagc agatagctga
    19561 attaaaacaa ctaggattac aaaataattt aaggattgca tttcctcaaa ctagctatgg
    19621 agaatatcta gaatggttgg gagaatgcaa aggagtattt aaaaatcaac caactaaatc
    19681 ggttggtatg gttacattta atggtgctca aggaactatc attacaaaag gaactattgt
    19741 tacaactata gctacagatg aaaaacagag catagaattt gagcttcttg aaactaaaac
    19801 tataggagca aatgaaacag tagatattaa agcagaatgt aggattgcag gaactatagg
    19861 aaatgtatct aataacacta taactgtttt actaggttct attagtggtg ttaaatcagt
    19921 ttctaataaa gaagatttca gaggtggaac agatatagaa gatgaagaac attttagaga
    19981 aagagttctt gtagcagagc aagaggacaa attaagtgga gctagttcag actatataag
    20041 atgggctaaa gaagtagatg gagtgggata tgcttatgta gttcccgaat ggaatggagc
    20101 agggacagta aaagtattaa tactagataa aaacagaaaa gcagcaacac aagaattaat
    20161 agataaggtc caagaatata tatatccatt gaatatatca gaaggagaaa atagagatgg
    20221 gaaagctcct atcggtgcat tagttacagt tgtgacacct gacacattac ttattaatgt
    20281 aaaagctagt tttatattta gtaatggctt tagtgaagaa actgtattaa acaatctaaa
    20341 aactaagata gataaatatt tagataagat tgatttaggg gggacagtct catacaatgc
    20401 tatacaggcg atagtaggct ctatgatgct gacagatgaa ggtatagaag acttttctaa
    20461 tcttactata aatgatgtaa aagaaaatat aaaattgcaa gaccaagtgg tcggaatagg
    20521 ggaaatagtt aacgaggtgg ttggatgata gcttctaaaa aaggtaaaga aatgcttctt
    20581 acattatctc ctatctatga acaatctatc ataatgcaaa gcttatatga agctatagga
    20641 agcgaatttg ataatctaga attattaaat aaagaaatag agttacaatt attccctcag
    20701 agtgcgacat ggggacttga attttgggaa aatagagtgg gtttatccac taatatagat
    20761 gaagatatag aagctagaag aagaaaggtc attgctaagc ttcaaagtaa atatattatg
    20821 acacctaaaa gaatggctat gatactccaa tcttatacag gtgcaaacat aaaaataaat
    20881 gaaaatatat ctccatatac ttttggtgtt gaattaacca gtacccaagg ttttcctaaa
    20941 gatttagaag atttatataa gagagtaaat gttataaaac cttctcattt agctgtaagt
    21001 tataagttag tttctttatt gaaaagtaaa acctattttg cacaaacggc aattatgagc
    21061 gaagaaataa ctgtatatcc gtacacaagc aaagaagtaa aagcaagtgt taaagccaag
    21121 tttgcactag ctcataacat gagctcagaa acattaacag tatatccaag ataggaggtg
    21181 gcataaatgg ctgatgaaca attttacaca atactaacaa acataggtaa agctaagatt
    21241 gctaatgcag gaatgttagg taagtcagta attttagaga agattcaagc aggtgatggt
    21301 ggaggaaact actataatcc aacagaagac caaacagcat taaaaaataa agtttgggaa
    21361 gggaatataa atgcttttga taatgatgaa aataatccta actggattat tgcaacagca
    21421 tgtatccctg gttcgatagg tggatttaca gttagagaaa tgggtcttat agataatgaa
    21481 ggagatatga ttgcagtttg taaaagccct gaaacctaca agccaaaagt tgataatgga
    21541 gctatgaaag atttgtattt gaaatttatc atagaagtat ctaatgtaga gaaagtgaca
    21601 ttagttgttg accctacagc tatttttcta actaaaaaag atgaagaaaa aatattaaca
    21661 aatattaata aactagacac taaaatagat acaaccaaaa cagaattaac aagcaacata
    21721 gaaactacta aaacagagct aaacactaga attgacacag aaaatgagaa acaaaatatt
    21781 aaaattgacc aattaatcgc aggtggttca aatgtggcat ctactcaaat aataacaatt
    21841 ga
//
LOCUS       DAEPIN010000063        21782 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.063, whole genome shotgun sequence.
ACCESSION   DAEPIN010000063 DAEPIN010000000
VERSION     DAEPIN010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21782
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.063"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            230..1531
                     /locus_tag="KR250_002792"
     CDS             230..1531
                     /locus_tag="KR250_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBH0068710.1"
                     /translation="MGKSKDVEIEKSILGTILLDNKLSYKLDELNENMFMNDICLEIF
                     KIMKELKKENIVIDVATVKSKIDRKSLAIKTSDVTNLITWGQNFGLDGHIKILKENLA
                     RRSINQNCQNLLHSLNLGENIDTCIYKFESNIKEILDKDTYENDDVNSIAGKVLDFLE
                     NKKDIGFKFGIKLLDTTIGGLFKGELTTIAAKSGVGKTALALQIMLNSFKQGKKTLFI
                     SREMTSEQVFMRNICRVTGVSTRNMKSKEIDENDWKLIVNAIGDLSENNLIYINDKID
                     TISAIRKRIRQVKPDLLIVDYVQLLTSQKSMDKREREVATFSRELKNMTLDFNIPVIQ
                     LSQLNDEMKDSRPYGDRPMRDSKAIYHDSNNVVYIHQLKGSDYEEAVRDIGESEEAVR
                     ASEYRGIKMVDLIVAKCRDGQTRHKHFCYFGDKLHFQELNY"
     gene            1607..1882
                     /locus_tag="KR250_002793"
     CDS             1607..1882
                     /locus_tag="KR250_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBH0068711.1"
                     /translation="MLELRKIIELLEEHKIEVSIPKELHKYSLLEYLELCQQSGGTYF
                     YIKTLEGALKEARNELMIKLFNGKNYMELARMFEMSVINVRHIVRKK"
     gene            3325..3555
                     /locus_tag="KR250_002794"
     CDS             3325..3555
                     /locus_tag="KR250_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBH0068712.1"
                     /translation="MSNKKKKYANILANECVACGSCIKACPRSAISVPCGISAKINRD
                     LCVGCGICEKICPASVIEIITILKEEEGKCHE"
     gene            3548..4129
                     /locus_tag="KR250_002795"
     CDS             3548..4129
                     /locus_tag="KR250_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBH0068713.1"
                     /translation="MNNKKHWYDYLWIFSSIYLILGFVNIIFAWIGLICFFVPLAISI
                     VKGNKAYCNKYCGRGQLFNLLGNKLKLSRNRDIPKFIRSKWFRYGFLTFFMVMFANML
                     FSTYLVFEGSRNFKEVVTLLWTIKMPWQWAYHGTLVSPWVVQFAFGFYSVMLTSTVLG
                     LITMILFKPRSWCVYCPMGTMTQMICKAKSKSI"
     gene            complement(4704..5381)
                     /locus_tag="KR250_002796"
     CDS             complement(4704..5381)
                     /locus_tag="KR250_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBH0068714.1"
                     /translation="MISQNEIDILTHSLPFWDKLTDLQKELLISSANTSHYKKGNPVH
                     CGDSDCIGILIIKSGTVRTYILSDEGREVTLFRLDDGDVCILSASCILKTITFDVYVD
                     AETDCDIIQISSSVFAKLSTENIHAELFSYKLATERFSDVMWAMQQILFMSFDKRLAS
                     FLIDEIAKNGSSTINMTHEQIAKYMGSAREVVSRMLKYFAREGIVSLSRGGIKVLDKD
                     KLRSLTL"
     gene            complement(5570..6934)
                     /locus_tag="KR250_002797"
     CDS             complement(5570..6934)
                     /locus_tag="KR250_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBH0068715.1"
                     /translation="MKESNKRIELLGSAPISKALLSMGLPTMIGMMITALYNLVDAYF
                     VGGLGTSQMGAISVAFPLGQLVVGLGLLFGNGAASYIARLLGHGDNEKAGKVVSTSIY
                     SSIIVGTLVIIVIMIFLNPILKLLGATKSIMPYAISYASIYVVSSIFNIFNVTMTNIV
                     TSEGAAKTTMCAMLLGALLNMILDPIFIYTFGLGVRGASIATAISQIASTLVYVGYIL
                     SKKSIFSFRINECCFSKEIFSEILKIGIPTLIFQLLTSLAITLTNMEATKYGDSVIAG
                     MGAVTRITSLGSLVVFGFIKGFQPIAGYNFGSKNYDRLHIAIKTSILWTTIFCVVLGL
                     ILILFPKSIISQFTSGDILLIENGSKALRANGFSFMLFGFYTVYSTLFLALGKAKEGG
                     FLGMCRQGVCFIPIILIIPVFYGLNGILYAQPIADLMAAIIAGFMSIRLHKELNLLKI
                     NGMH"
     gene            7062..7583
                     /locus_tag="KR250_002798"
     CDS             7062..7583
                     /locus_tag="KR250_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBH0068716.1"
                     /translation="MATIDLIVLGIVKQQPKSAYDIQKAVEYRNISRWVKISTPSIYK
                     KVIQLEQKGYIESKTVKNGKMPEKAVYSLTELGETHFLDLMFDTSSKPINILLDFNAV
                     IVNLNSLTPEKQKLCLNNIEENVQELKKTIEENISIKTEVPDTAKAVLEQQLVLAQAL
                     ETWINSLKDNMGN"
     gene            7715..7852
                     /locus_tag="KR250_002799"
     CDS             7715..7852
                     /locus_tag="KR250_002799"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0068717.1"
                     /translation="MLNFLRARARARARARARARARARARARARARARARARARAISK
                     V"
     gene            8070..9149
                     /locus_tag="KR250_002800"
     CDS             8070..9149
                     /locus_tag="KR250_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HBH0068718.1"
                     /translation="MPELLKNMYNRESLYEVAVAIQSVYNSFKVDEFIKSTMDETWNN
                     LELKARCRKISMSLGMYLPEDYKEALSILEKSVTGFYFAFFFPDFVEVYGQDDINWDL
                     SISALERNTEYWSSEFAVRAFIIKDEERMMAQMRKWSKHKSEHVRRLASEGCRPQLPW
                     GQAISKFKKDPTPVLPILEQLKTDTSTYVQKSVANNLNDISKTHPDLVISIAKDWYGK
                     NKSTNWIVKHGCRTLLKKGNRDVLALFGYDDTTSINLQDFTLETTSISIGEDLTFSFN
                     ILAKKATKTRLEYGIDYIKSNGKRNRKIFKISEVSLKENEKKSYMKKHSFADVSVRKH
                     YPGIHSIAIIINGIEKDKLDFELGI"
     gene            9473..9841
                     /locus_tag="KR250_002801"
     CDS             9473..9841
                     /locus_tag="KR250_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HBH0068719.1"
                     /translation="MDVYSFWNDTLAQDAVKIRKYFHENAYINWHDTNEHFTLEEFII
                     ANCEYPDKWNGKVEKVLEFGNLVITVTHVYAINKPLSFHVVSFISLKDDKIISIDEYW
                     GEDNDVPQWRLDKHIGKPIK"
     gene            9958..10761
                     /locus_tag="KR250_002802"
     CDS             9958..10761
                     /locus_tag="KR250_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBH0068720.1"
                     /translation="MEEKYILSDRGKVYYWIKESNSVFAKNLIFLPGLTANHHLFDFQ
                     IQYFSKNHNVLVWDAPAHGKSRPYLDFSYSNLVEELKVILDKQNMKKVVLIGQSAGGF
                     VAQSFIVKYPNMVEGLMMIGTCPYGTDYYSKSDIFWLKQTEWMLKLFPDKFLRNIISK
                     MCGATSEGRRSMMKMLEGYEKKELCHLMYLGFAGFIPEIQDLNIHCPVCLVVGDKDKT
                     GKVRKYNEQWHNKTGYPLHIIKNASHNVNVDKPNELNQIMDIFIKQLNI"
     gene            11146..12036
                     /locus_tag="KR250_002803"
     CDS             11146..12036
                     /locus_tag="KR250_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBH0068721.1"
                     /translation="MIESIPAKQILQKVKFDNTRWFGIDYNMNLYRGCSHGCIYCDSR
                     STIYNIENFDKVRYKENVIEILSKELRSKRKKGVVGIGAMSDTYNPFEKQLCITKQAL
                     DLISENHFGVSIDTKSSLVVRDIPILQKIKKNNSAIVKLTITTANDELSKKIEPYVNP
                     SSVRFEAVKELNDSGIFCGILLTPMLPFLTDTKDEIRAIVEKAHKANAKFIYCMYGVT
                     MRSGQREFFYEHLRDISPKLVFKYQKTYGLNYVCTVQNKDCCEKLLREECARYGILTD
                     MKDIIKAYKRSESYTQIKFF"
     gene            12599..13042
                     /locus_tag="KR250_002804"
     CDS             12599..13042
                     /locus_tag="KR250_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBH0068722.1"
                     /translation="MIEIKTIEINKKSQIDLLILADPCEEMIDKYLNKGTMYALYDNK
                     ELTCIAVVNEISKEICELKNIATYEHFQNMGYASKMIYHLLDVYSKKYSSMVVGTSES
                     GVPFYEKFGFVYSHKIKNFFVDNYPEPIFEGELQCVDMLYLSYNF"
     gene            13214..13384
                     /locus_tag="KR250_002805"
     CDS             13214..13384
                     /locus_tag="KR250_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0068723.1"
                     /translation="MDSNNNTIKSTVKKGISFGSCLAMIISYTAWKSIPWAIFHGLMS
                     WIYVLYYWVKYA"
     gene            13757..14878
                     /locus_tag="KR250_002806"
     CDS             13757..14878
                     /locus_tag="KR250_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HBH0068724.1"
                     /translation="MKEKLEEKSKIIKTSKEYKRTIIEEKDREWTQKLNDILSRGDFS
                     NEKLDKIKDLIPKEILTSENVGEVVTEDGYAKPEYDEVFKSLFTLNNDYDLLANFIND
                     ILKDAPYANRNIKQFTQIKRIIKVETDPTINYIGEKRPRLDILAEDEERNHINIEMQR
                     ALEEDYLERAEYYLSRVHGRKLEEGKEYKEIGKTVGIHILNHVKYNHIEDYVNCLRLT
                     MDGHPDIFSSKTALYFIELPKIRKSSCIANRVLIWGKLIDNPSHIDIRILSKTDYVIK
                     RALDRLKELGSNEDYLLNLKRGAYIMNKSRNFKEEIFQEGIEKGIEKGIEKGIAKGKR
                     EEKFNIIIKLKNKGYNLSEICDIIDDLNKSEVEKVYNQN"
     gene            15083..15571
                     /locus_tag="KR250_002807"
     CDS             15083..15571
                     /locus_tag="KR250_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3841 domain-containing protein"
                     /protein_id="HBH0068725.1"
                     /translation="MKFYTIQTLEFWNNNKNNKYLSNDYSFILEDFDIPYKWMYSQMT
                     NRIYEFDDSMIWVWTKRPDLRCSGYLEKGKKGVLLEIEISEDNVLLSEFDLWHLPLMD
                     ITVELYEDEQIEKKVSWERIFDLELCSKLNEVNIEDLTLQGVTSKVPLSNVKLIKEFV
                     AK"
     gene            15657..16064
                     /locus_tag="KR250_002808"
     CDS             15657..16064
                     /locus_tag="KR250_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0068726.1"
                     /translation="MSKQQTGIKSTLISEILGISYCIGATLVNKYFFKGSSIEYLPII
                     IWVLAMGAQWVYNKKYDDLIDEASKCILSRVNDIAIKVLFFSVAIVSIFLVTLDINVS
                     NLNIGMSLLVILFIQSLLKLILFIYFDRKGIYN"
     gene            16065..16283
                     /locus_tag="KR250_002809"
     CDS             16065..16283
                     /locus_tag="KR250_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0068727.1"
                     /translation="MSVLLKTKIKSYREKNLMTQGELAKSVGVRRETIVHLENGRYNP
                     SLKLAMDIAKVFDTTVEELFEFIEENNI"
     gene            17357..17652
                     /locus_tag="KR250_002810"
                     /pseudo
     CDS             17357..17652
                     /locus_tag="KR250_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427875.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            18060..18749
                     /locus_tag="KR250_002811"
     CDS             18060..18749
                     /locus_tag="KR250_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0068728.1"
                     /translation="MDILLVEDDKNIREMVYDFLISENYSVKTAIDGNTAIEIFKNYS
                     FDIILLDLMLPKSSGFEVLKYIRSVSVVPVIITTAKDSDTDKMLGLNLGADDYITKPF
                     SIVELLARIKANIRRATVYSSSRNSQDMVIRYKDLIINISKYVVSKNGIDLNLTHTEF
                     EIVKLLATNQGRAFSKEQLYNAIWKEPYYGNENVLNTHINRLRNKLSDGIEKNNYIKT
                     LWGIGYKMEEE"
     gene            18749..19666
                     /locus_tag="KR250_002812"
     CDS             18749..19666
                     /locus_tag="KR250_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBH0068729.1"
                     /translation="MELFLVVISIVLFALYLKERNKSKKMESDYKYINSRLRDITNDD
                     EINYILVPSDISIVKETAVDINKLVEKFYSRQINYNRSEKAILQIFTNISHDLRTPIT
                     VLKGYIEMLYLQSQKEELSSSMRAIVEKMQTNSNELVNSINNLFNMAKIQSGDMILNL
                     QKSNLTQLCHEIILEFYDLLDKENFNVEVNIEDRPIYANVDTEALQRILKNLIDNAIK
                     YGSDGRFLGISLYEKEKHVFIDIEDKGSGISETEKEHIFTRAYTADRKKGNGLGLAIS
                     QGLANSMGGSIYVNSRPNVKTVFTVKLKS"
     gene            19739..20665
                     /locus_tag="KR250_002813"
     CDS             19739..20665
                     /locus_tag="KR250_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0068730.1"
                     /translation="MNYIIETHQLKKIYKDKAVVNAVNIHVKKGEIYGFVGPNGAGKS
                     TVMKMLLNLVNPNSGEIVMFGKKVAETDFEILKKIGTIIENPYLYENLTAKQNLDLHC
                     EYMGYYNKEHISEVLECVGLSKQSNKKVSKYSLGMKQRLAIARAILTKPELLILDEPI
                     NALDPEGIREMRELFRKLNTDYGITIFISSHILSEVEQIADTIGIIQDGKLIKEISMS
                     DIHKYQTDYVEVDVDNVELAGYLLEKEFGIKDFKITSESCIEIYDLRKDVKEISKIFI
                     QNQIGINSIGRKQSSLEDYFFQTTGTGGKETL"
     gene            20662..21342
                     /locus_tag="KR250_002814"
     CDS             20662..21342
                     /locus_tag="KR250_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0068731.1"
                     /translation="MIHLMKLELKKVGLKRYFLFSMLGIFLCMFFVFTGLNDKSTSIY
                     DYDVTFRTISLFFCFYYIILFAVLNVAYIINEYTNKTILILFSYPLDRKKLILSKLLL
                     ITTLMISSMIVGYICCFTFVVVLDKYFNLVIGDFHISILSYWIPGAIKSIIVFCSLGI
                     WTFVVGMIRKSIPVTLVSSIIFINLRQFILAGTNTTQDSLISVFIIVGITVIGIYYIL
                     NHKVTEID"
     gene            21440..21775
                     /locus_tag="KR250_002815"
                     /pseudo
     CDS             21440..21775
                     /locus_tag="KR250_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427885.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
BASE COUNT         8575 a         2254 c         3753 g         7200 t
ORIGIN      
        1 atatattaat aattggtagt aaagtatatc aaataagata ttgctattat gtagtaaatt
       61 atataaaaca atgtatcaga ataaatagta tattaataat ttgtagtata tcatgacgaa
      121 taatacagtc ataacaaata gtgagtcata acaaataatg tatttataat tgataatgta
      181 tttaaaaatc aaaatataaa aacttataag gaaagagaga ttaatttata tgggtaaatc
      241 aaaagatgtt gaaatagaga agtctatttt aggcacaatt cttttagaca ataaattaag
      301 ctataagcta gatgaattaa atgaaaacat gtttatgaat gatatatgtc ttgagatatt
      361 taaaattatg aaagaactta aaaaagaaaa catagtgatt gatgtggcca ctgtgaaatc
      421 aaagatagat agaaaatctc tagcaataaa aacgagtgat gtgactaatc taattacttg
      481 gggtcagaat tttggtcttg atggtcatat taaaatttta aaagaaaatt tagctagaag
      541 aagcataaat caaaattgcc aaaacctttt acacagccta aaccttggag aaaatatcga
      601 tacttgtatt tacaaatttg aaagtaatat aaaggagatt ttggacaagg atacttatga
      661 aaatgatgat gtaaatagta tagcagggaa ggtgcttgat tttttagaaa acaaaaaaga
      721 tattggattt aaatttggta taaaattact tgataccact ataggaggtc tttttaaagg
      781 agagctaacc acaatagcag ctaaatcggg ggttgggaag actgctttgg cacttcaaat
      841 aatgttaaat tcatttaagc aaggtaaaaa aactttattt ataagtagag aaatgaccag
      901 tgagcaagta tttatgagaa atatatgcag agtaactggt gtaagtacaa ggaatatgaa
      961 atctaaagaa atagatgaaa atgattggaa acttattgta aatgctatag gtgatttaag
     1021 cgaaaataac ttaatttata ttaatgataa gatagataca attagtgcaa ttagaaagag
     1081 gataaggcag gtaaagccag atttattaat agtggattat gtgcaactcc ttacatctca
     1141 gaaaagcatg gataagagag aaagggaggt agctactttt tcgagagaac ttaaaaatat
     1201 gactttagat tttaatatac ctgtaatcca attaagtcag ttaaatgatg aaatgaaaga
     1261 ttcaagacct tatggagata gacctatgag ggatagcaaa gccatctacc acgatagcaa
     1321 caatgtggtt tatatacatc aattaaaggg gagtgattat gaagaagcag tgagagatat
     1381 tggagaaagt gaggaggcag ttagagcatc agaatacagg ggaattaaga tggttgattt
     1441 aatagttgcg aagtgtagag atggacagac taggcataag catttttgtt actttgggga
     1501 taagttacat tttcaagaac taaattatta ggtcaataaa gtagtaataa caagcttatt
     1561 aaaagtcggg aaatagcaaa tagattataa aatatgaggt gaaaatatgt tggaattaag
     1621 aaaaattata gaattacttg aagaacacaa gatagaagta agtattccaa aggagttaca
     1681 taagtatagt ttattggaat accttgaatt gtgtcaacaa agtgggggaa catattttta
     1741 tattaaaaca ctggaagggg ctttaaaaga ggctagaaat gagcttatga ttaaattgtt
     1801 taatgggaaa aattatatgg aattagcgag gatgtttgaa atgagtgtta ttaatgtcag
     1861 gcatattgtt agaaaaaaat agatattttt ggagaataca agtgtgatat acttctgaaa
     1921 tagaagttat tatagttgaa ataaaattgt ggttaataaa ttaaagttgt gtataagttt
     1981 aagattatag aggagtataa gtgtgattaa cagcatgatt ttgtgaataa aattgaggat
     2041 atatatgagt taaatgtgtg attaataagg taaggttgtg tataagatta tgagtattta
     2101 aaaggagggg ctataattaa tagtgtagag ttgtgtgttt gcttaagatt atataataat
     2161 ttaatagatt aaactatact aataaggtaa aatcgtgtgt ttagttaaag cttattataa
     2221 aatcttgata tgtgattaat gatgcaatat gtgaataaag ttaagattat aatagattta
     2281 acctgtggct aatagaataa aattgtgaat aaagttaaga ttataatagg tttaacctgt
     2341 gattaataga gtaagagtgt aaatagagat aaacttcaat atgagcttaa cctatgatta
     2401 atagagtaag agtgtaaata gagataaact tcaatatgag cttaacctat gattaataga
     2461 gtaagagtgt aaatagagat aaacttcaat atgggtttaa cttgtgatta atagagtagg
     2521 gtgtaactaa agataaactt caatatgagt ttaacttgtg attaatagag taaggtgcaa
     2581 ctaaagataa acttcaatat gagcttaacc tatgattaat agagtaagaa tgtaaataga
     2641 gataaacttc aatatgggct taacttgtgg ttaatagagt aagagtgtaa ctaaagataa
     2701 acttcaatat gggtttaact tgtggttaat agagtaagaa tgtaaataga gataaacttc
     2761 aatatgagct taacttgtga ttaatagagt aagagtgtaa atagagataa acttcaatat
     2821 gggattaact tgtgattaat agagtaagag tgtaactaaa gataaacttc aatatgagct
     2881 taacctatga ttaatagagt aagaatgtaa atagagataa acttcaatat gggtttaacc
     2941 tgtggttaat agagtagggt gtaactaaag ataaacttca atatgagttt aacttgtggc
     3001 taatagaata agattgtgaa taaagttata gttttagatg ggtattaacc tgttactaat
     3061 agaatatgat taggtgtaaa attatagagc tgtataagaa aatattatag atatattaaa
     3121 tattagataa taaatattta tatagataca caaaagaaaa ggcgatatga agctaaactt
     3181 aatgacttca tatcgttttt tatttgaaac tcctttgtat ttagagtgtg atttttaata
     3241 ttgaggtttt atgtgattaa gtcacagata aaaagtaagt atcttgatat aattcttata
     3301 gattaataat atagggaggt tattatgtct aataaaaaaa agaagtatgc aaatatttta
     3361 gcaaatgagt gcgttgcttg tggtagctgt ataaaagcat gtccaagaag tgctatctca
     3421 gttccatgtg gcattagtgc aaaaattaat agagatttat gtgttggatg tgggatttgt
     3481 gaaaagatat gtccagcatc tgtaattgag attataacaa tattaaaaga ggaagaaggt
     3541 aagtgtcatg aataataaaa aacattggta tgactattta tggatttttt cttcaatata
     3601 tttgatattg ggatttgtaa atattatttt cgcttggatt gggctaattt gtttctttgt
     3661 tccactagct atttctattg ttaagggaaa taaagcttac tgtaataagt attgtggaag
     3721 agggcaactg tttaatttgc ttggaaataa attgaagctg tcaagaaata gagatattcc
     3781 taaatttatt agaagtaagt ggtttagata tgggtttttg acatttttta tggtaatgtt
     3841 tgccaatatg cttttttcaa cttatttagt gtttgaagga agtagaaatt ttaaagaagt
     3901 agtgactttg ttgtggacta taaaaatgcc ttggcaatgg gcgtatcatg gaactttagt
     3961 ttcaccttgg gtggtacagt ttgcatttgg tttttatagt gttatgctta catcaactgt
     4021 tcttggtttg attactatga tattgtttaa acctcgttca tggtgtgttt attgccctat
     4081 gggtactatg actcagatga tatgtaaggc aaagagtaag tctatataga aaggctttat
     4141 gtgatattta cagttttgtt agagatgagt agtaggtttg aatatatatg acaaatatat
     4201 gagtataggg gcatagattt agtttgtgta aggtaaactg atttatgctt cttttttatg
     4261 tcttagatta ttggctaagt ggaaacaatg aatttagata ttaagaatta ttcagttatt
     4321 tgaagaaaat agaataataa ttaacattat atgaatggat attcattaca tataagccag
     4381 ttaatagcaa gatttattta atatgaaata ggtcaagtat ttaaactaaa taaggtcatg
     4441 acaattaata taagtataga tataaaaaga ttagaagtta taacttataa aaggtattct
     4501 atataaatag tatgtggaaa atagtcaaat taacttatta gaaacaaaaa atatacataa
     4561 aaaaataaat gtaaatatca aataagatac tatataaaaa tagatataag tatctttttt
     4621 attattgaaa ttatgaaaat atatttgatt tcacacaaaa aagagtgcct catgaactaa
     4681 aaagttcatt ttgagacaca cttttacagt gtcaaagaac gaagtttatc cttatccaat
     4741 acttttatac caccacgaga taaagacaca ataccttctc tagcaaaata tttcaacatt
     4801 ctcgatacaa cttctctggc actacccata tacttagcaa tttgctcatg agtcatattt
     4861 atagtgctac tgccattttt agctatctca tcaattaaaa aagatgccaa acgcttatca
     4921 aaactcataa aaagaatttg ttgcatagcc cacataacat cagaaaaacg ttcggtagct
     4981 aatttatatg aaaataactc tgcatgaata ttttctgtag ataattttgc aaaaacagag
     5041 gaactaatct gtataatatc gcagtccgtt tcagcatcta catagacatc aaaagttatt
     5101 gtttttagaa tacaggaagc tgataaaata catacatctc catcatcaag tcggaacagt
     5161 gtaacctctc gaccttcatc tgaaagtatg tatgtacgta ctgttccaga ctttataatc
     5221 aaaataccaa tacagtcact atcaccacaa tgtactggat tccctttttt gtaatgcgag
     5281 gtatttgctg aagaaattaa taattctttt tgtaagtcag taagtttatc ccaaaaaggc
     5341 aagctatgtg taagtatatc gatttcattt tgtgaaatca tacaatatct cctttcttaa
     5401 attgttataa tcccatgata caagtgaata gataaattga caagtagttt gttaatagga
     5461 ttatttagtc agatagtaat agtatgattt atgtagaaaa aatatgataa tatatattct
     5521 tgctattaat gagaaggtaa tatgttttga tgatagtagg tttgtataac taatgcatac
     5581 cgtttatttt taaaagatta agttccttat gtaaccttat agacataaaa ccagcaatta
     5641 tagcagccat caaatcagct attggttgtg catataagat accatttaac ccatagaata
     5701 caggaataat caaaattata gggataaaac agacaccttg tctacacata cctaaaaatc
     5761 caccttcctt tgcttttcct agtgcgagga ataatgtaga atacactgta tagaatccaa
     5821 atagcataaa tgaaaaccca tttgccctaa gagctttact accattttct attaatagaa
     5881 tgtcaccact tgtaaattgt gaaattatag attttgggaa gagtatcaat ataagaccaa
     5941 gcactacaca aaatattgta gtccataata tggaagtttt tattgctata tgtaatctat
     6001 cgtagttctt tgaaccaaaa ttataaccag caattggttg gaaacctttt ataaaaccaa
     6061 atacaacaag actacctaaa gacgttattc gtgttacagc acccattcca gctatcacag
     6121 aatcaccata ctttgtagcc tccatattag ttaatgtaat ggcaagacta gtcaataatt
     6181 gaaatattaa ggtaggtatg ccaattttta ggatttctga aaaaatttct tttgaaaaac
     6241 agcattcgtt aattctaaaa ctaaatatac ttttcttgct taaaatatat cctacataaa
     6301 caagtgttga agcaatctgt gagatagctg ttgcaataga agcacccctt acccccagac
     6361 caaaagtata aatgaatatt gggtctaaaa tcatattaag caaagcacct aataacatag
     6421 cacacatagt tgttttagca gctccttcac ttgtgacaat attagtcata gtgacattga
     6481 atatattaaa aatagatgac acgacataaa tgctagcata agatattgca taaggcataa
     6541 tactttttgt agctcctaac aattttaaaa ttggattgag aaaaatcata atcacaataa
     6601 taactaatgt acccactata atactactgt aaatagatgt acttaccact tttcctgctt
     6661 tttcattatc accatggcct aaaagtctgg caatatatga agcagctcca tttccaaaca
     6721 ataatcctaa tcccacaaca agctgaccta atggaaatgc aacggatata gctcccattt
     6781 gacttgttcc aagtccaccc acaaagtatg catcaaccaa attgtataat gcagttatca
     6841 tcatacctat catggtaggt aatcccatag ataacaatgc ttttgagatt ggggcagacc
     6901 ctaataattc aattcttttg ttactttctt tcatacttaa cactcctctt ctattgacat
     6961 taagtctaaa ttataatact ataaattata gtaagtatgg aaaatagtac tataatttat
     7021 agtatttgtc aatagtaaga aaactaaagg agatgatttt tatggctaca atagatttaa
     7081 ttgtattggg aatagtaaag caacaaccca agagtgctta tgatattcaa aaggcagtgg
     7141 aatacaggaa tatatcacgt tgggttaaaa taagtacacc atcaatttat aaaaaagtaa
     7201 ttcaattaga acaaaaggga tatattgaaa gtaaaactgt gaagaatgga aaaatgccag
     7261 aaaaagcagt ttattcatta actgaattag gtgagacaca ttttttagat ttaatgtttg
     7321 atacatctag caaaccaata aatattcttt tagattttaa tgctgttata gtaaatttaa
     7381 atagccttac accagaaaaa cagaaattat gtctcaataa tattgaagag aatgttcaag
     7441 agttaaagaa aactattgag gaaaatataa gtataaagac agaagtacca gacacagcaa
     7501 aagcagtgtt ggagcaacag ttggtgctag cacaagcatt ggaaacctgg ataaattctt
     7561 taaaagataa tatgggaaat tagaattaga gaagctaact taataatcat taaacaaaaa
     7621 ccagcatatt taataatgag atattagaaa aaataaaaca aatgatataa actgaaaagt
     7681 atatttaaaa taaagctcac ttaattgagg gagaatgtta aactttttaa gagcaagagc
     7741 aagagcaaga gcaagagcaa gagcaagagc aagagcaaga gcaagagcaa gagcaagagc
     7801 aagagcaaga gcaagagcaa gagcaagagc aagagcaatc agtaaagtat aaaattctat
     7861 tactataata aaagatagaa ctagaagtct ttatgaaagt gggaaaagta tttatataaa
     7921 taaattcata tagtttatag tttaatagta ggaaatatcg tatgagtaag aatattgtat
     7981 tttacataaa gatttagcaa tatgacacac tgttgtcgtg ttctgtagga tatgattaat
     8041 aaatcagata tgaaaggaga gtgagtaata tgccagaatt actaaaaaat atgtataatc
     8101 gtgagtcact ttatgaagtt gcagtagcta tccaatcagt ttacaactca tttaaggtag
     8161 acgagttcat aaaatctaca atggatgaga catggaataa cttagaatta aaagccagat
     8221 gtcgaaagat aagcatgagt ttaggaatgt atttaccaga agactataag gaagctcttt
     8281 ccattcttga aaaatcagtt acaggttttt attttgcttt tttctttcca gattttgttg
     8341 aagtttatgg gcaggacgac ataaattggg atttatctat aagtgcatta gaaagaaata
     8401 ctgagtattg gtcttcagaa tttgctgtaa gagcatttat catcaaggat gaagaacgta
     8461 tgatggcaca gatgcgtaaa tggtctaagc ataagagcga acatgtacga agacttgcaa
     8521 gtgaaggttg tcgtccccag ttgccttggg gacaagcaat atccaaattt aaaaaagacc
     8581 ctacacctgt gctacctata ttggagcaac ttaaaacaga tacatcaacg tatgtacaaa
     8641 agagtgttgc taataatcta aatgatatct caaaaacaca tccagattta gtcataagca
     8701 ttgcaaagga ttggtacggt aaaaataaat ctactaactg gattgtaaag catggttgta
     8761 gaactctact aaaaaaagga aatcgtgatg tattagccct ttttggatat gatgatacta
     8821 cttctataaa tcttcaggac tttactttag aaacaacgtc tatttctatt ggagaggatt
     8881 taactttttc atttaatatt ttagcaaaaa aagcgacaaa aacaaggctc gagtacggaa
     8941 ttgattatat taaatctaat ggaaaaagga ataggaagat atttaaaata tcggaggttt
     9001 cattaaaaga aaatgagaaa aagtcatata tgaaaaaaca ttcatttgca gatgtaagtg
     9061 taaggaaaca ttatccaggt atacactcga ttgctattat tattaatggt atagaaaaag
     9121 ataaacttga ttttgaatta ggtatataat tttactaaca gaataatcta tatcagatac
     9181 tttgctaaac ttatataata tattttacta aatttatatt aggtagttta ctacttgttt
     9241 actgaattta aatacaaaga aatacaggtg ctaatccctg cccattaaag gaatatgagc
     9301 ataacacaaa agtagaactt atcaattctg ttacgggtat agtagtaaat ccatagaatc
     9361 acaattagaa gatgaagtgt aaaaaataag ttagtgttga ttttagttgc tagtgatatt
     9421 gaatagttat acaatagcta tataaattag aatttgtaga ggagaaatgt aaatggatgt
     9481 atatagtttt tggaatgaca ctttagcaca agatgctgtt aagataagaa aatattttca
     9541 tgaaaatgct tatattaatt ggcatgatac aaatgagcat tttactttag aagaatttat
     9601 tattgcaaat tgtgagtatc ctgataaatg gaatggtaaa gtagaaaagg tgctagaatt
     9661 tggaaattta gtaattacag tgacacatgt ttatgcaata aataaaccac tatcatttca
     9721 tgtcgtttca tttatcagcc ttaaagatga taagattatt tctattgatg aatattgggg
     9781 agaggataat gatgtgccac agtggagatt agataaacat attggaaagc caataaaata
     9841 gtttttgttc tgttttataa ctgaaatttt gataacattt gatattttga tttgtcattg
     9901 gtcaacatag tcgttagtgt aataatttaa aaaagtagaa tttggagagt gaacactatg
     9961 gaagaaaaat acatcttatc tgaccgaggg aaagtatatt attggataaa agaaagtaat
    10021 agtgtgtttg caaagaactt aatttttttg ccaggactaa cagcaaatca tcatcttttt
    10081 gatttccaaa tacagtactt ttcaaaaaat cataatgtat tggtatggga cgctcctgct
    10141 catggaaaat caagacctta cttagatttc tcatattcta acttggtaga agaattgaaa
    10201 gtaattttag ataaacaaaa tatgaaaaag gttgtactta tagggcaatc ggcaggggga
    10261 tttgtggcac agtcctttat tgtaaaatat ccaaatatgg tagaaggact aatgatgatt
    10321 ggaacttgtc catatggaac tgactattat agtaagtcag atattttctg gttaaaacag
    10381 actgaatgga tgttaaagtt attcccagat aaatttttgc gaaacataat atctaagatg
    10441 tgtggagcta cgtctgaagg cagaaggagt atgatgaaaa tgttagaagg ctacgaaaaa
    10501 aaagaactgt gtcatctcat gtatctggga tttgctgggt tcataccaga aattcaagac
    10561 ttaaatatac attgtccagt ttgtttggtg gttggagata aagacaagac gggtaaagtg
    10621 agaaaatata atgagcagtg gcacaacaag actgggtatc cattgcacat tataaaaaat
    10681 gcttcacata atgtaaatgt cgataaacct aatgaactaa atcagattat ggatatattt
    10741 attaagcaat taaatatata aaaattacat actcattagg taaattatgt agtatatgtg
    10801 tttatagatg gttatagaaa taaatatgta ataaactact taacacttaa aaagtggatt
    10861 tgtccagagc ataatacaaa gcatgatata taaattttta agcgaaatac atcaaaagaa
    10921 gtattagaat tagtggagat aaaacagcta ttagagatta taaataaata gttttaaagg
    10981 tactacgaat atagaggaag gtaaatcata cgttattcaa ggattataaa tgattagttt
    11041 aaaagtacta tcggacaggt ctctgcatat ttaaacctta gtaaaggtag ttaaagtagt
    11101 agtttaaaaa ctaaaaaatt atataaagga attaggtata agaaaatgat tgagagtata
    11161 ccagcaaaac aaattttaca aaaagtgaaa tttgacaata caagatggtt tggaattgac
    11221 tataatatga atctctatcg tggttgttct catggatgta tatattgtga tagccgtagc
    11281 acaatataca acatagagaa ttttgacaaa gttagatata aagaaaatgt gatagagatt
    11341 ttaagtaaag aattgcgttc aaaaagaaaa aaaggggtag taggcattgg agctatgtct
    11401 gacacatata atccttttga aaagcaactt tgtattacaa agcaggcact agatttgatt
    11461 tcggaaaatc attttggagt gtctattgat actaaaagta gtttggtggt tagagatata
    11521 ccgattttgc agaaaattaa aaaaaataat tctgctattg taaagttgac tataaccact
    11581 gcaaatgatg aattatcaaa gaaaatagaa ccttatgtaa atccaagttc tgtacgattt
    11641 gaggcagtga aagaacttaa tgattctgga attttttgtg gtatattatt gactcctatg
    11701 cttccatttt tgactgatac taaagatgaa atacgagcaa ttgtagaaaa agcacacaag
    11761 gctaatgcaa aatttattta ttgtatgtat ggtgtaacaa tgcgtagtgg tcagcgagag
    11821 tttttttatg agcatttaag agatatatca ccaaaattgg tatttaaata tcagaaaaca
    11881 tatggtttaa actatgtatg tactgtacaa aataaagatt gttgtgagaa gttgttaaga
    11941 gaagaatgtg cacgttatgg tattttgacg gatatgaaag atattataaa agcgtacaag
    12001 aggtcggagt catatactca aataaaattt ttttaagata ggctttggat gagatataat
    12061 agtggtgatt gatgtgttga tattatagtt gattgttaga gaggatatta tagatgaata
    12121 ttagtattaa tattttaggt aattgctaga gctgatatta tagatgaata ttaatattga
    12181 tattctaggt aattgctaga gttagtatta tagatgaata ttagtattga tattttaggt
    12241 aattgctaga gctgatatta tagatgaata ttaatattga tattctaggt aattgctaga
    12301 gttagtatta tagatgaata ttagtattga tattttaggt aattgctaga gctgatatta
    12361 tagatgaata ttaatattga tattctaggt aattgctaga actaatatta tagatgaata
    12421 ttagtattga tattctaggt aattgctaga gctgatatta taatttatta ttaatattaa
    12481 tattctaggg aattatcact atattttaaa aagtaaaaat atatatcaaa atgaactaaa
    12541 taagtactgt aagaagtttg ttaaattaag gtttgaataa agtgagggtg gagaacttat
    12601 gatagaaata aaaactatag aaataaataa aaaaagtcaa attgaccttt taatattagc
    12661 tgacccatgt gaagaaatga ttgataaata tctcaataaa ggaactatgt acgcattgta
    12721 tgataataaa gaacttactt gtatagcagt tgtaaatgaa atttccaaag aaatatgtga
    12781 attaaaaaac attgctacat atgagcattt tcaaaatatg ggttatgcct caaagatgat
    12841 ttatcatctt ttagatgttt atagtaaaaa atattcatca atggttgttg gtacatctga
    12901 aagtggagtt cctttttatg agaagtttgg atttgtttat tcacataaaa ttaaaaactt
    12961 ttttgtagat aattatccag aaccaatctt tgaaggagaa ttacaatgtg ttgacatgtt
    13021 gtatctatca tataactttt agataggata atatttttag gttaatataa ttaattaatt
    13081 catataatta tcggaattat aatttataaa ttatgtataa taagagtata atttatttag
    13141 aagctgttat attgtattag attcaaataa gatatataat aacttgttta ttttgaattt
    13201 tggaggatta attatggatt caaataataa tactataaaa tcaactgtta aaaagggtat
    13261 ttcttttggt tcttgtttag caatgattat ttcttatact gcatggaaat ctattccatg
    13321 ggctattttt catggcttaa tgagttggat atatgtactt tattattggg ttaagtatgc
    13381 atagattaaa ttaataaata agtatgtaat attgtaaagt ttaagaaaca aaatgtaagg
    13441 gagtgtctcg aagtgaactt ttagtttata aggcactctt ttttgtatta aataaaatat
    13501 attttcatta cttaaacaat gaaaaagatg agtatctcta ttttaataca tacccttata
    13561 ttcattcatt tatcaacaat agttttaaaa agaaagctat ctaaaatcta ttgtaatcta
    13621 agtgcttctc tttaaagtct taactttagt aatgatatat aagtttataa ggttcttatt
    13681 tcaataatgc aaattttact tagaagttca gactttggta tgaagaaatg ggtaataata
    13741 aaaaagaggt gttgatatga aagaaaaatt agaagaaaaa tctaagatta ttaaaacatc
    13801 aaaagagtat aaacgaacta taattgaaga aaaggacaga gaatggaccc aaaaattaaa
    13861 tgacatactt agtagaggtg atttttctaa tgaaaaatta gataaaatca aggatttaat
    13921 tccaaaagaa atattgacta gtgaaaatgt tggagaagtt gtaactgaag atgggtatgc
    13981 taagcctgag tatgatgagg tatttaaatc attgtttact ttaaataatg attacgattt
    14041 acttgcaaat tttataaatg atatcctcaa agatgctcca tatgctaata ggaatatcaa
    14101 acagttcact caaataaaaa gaataattaa ggttgaaacc gacccaacaa ttaattacat
    14161 tggtgagaaa agacctagac ttgatatttt ggctgaagat gaagaacgta accatataaa
    14221 tattgagatg caaagagctc ttgaagagga ttatttggaa agagctgaat attatcttag
    14281 tcgtgtacat ggtagaaagt tagaagaagg taaagaatat aaagaaattg gaaaaactgt
    14341 tggaatacat atactaaatc atgtaaaata taatcacata gaagattatg taaattgcct
    14401 gagattaaca atggacgggc atccagatat tttttctagt aaaactgcat tatattttat
    14461 agaacttcca aaaattcgta aatccagttg tatagcaaac agagttctaa tatggggtaa
    14521 acttattgat aatccttctc atatagatat tagaatacta tctaaaacgg attatgttat
    14581 taaaagagca cttgatagat taaaagaatt aggtagtaat gaggactatt tattaaattt
    14641 aaagagaggt gcgtatatta tgaataaaag tagaaatttt aaagaagaaa tttttcaaga
    14701 aggtattgaa aaaggtatcg aaaaaggtat cgaaaaaggt attgcaaaag ggaaaagaga
    14761 agagaaattt aatatcatca taaaattaaa aaataaagga tacaatttga gtgaaatatg
    14821 cgatattata gatgatttga ataaatcaga ggttgaaaaa gtctataatc aaaactaata
    14881 ctatgattga gatggcacta gataaataca aagcttcctc ttttaaattt aaattaatat
    14941 aaaactttgt tatttaaata tggtagatat aaagagatat ataaataata ggtacaaaga
    15001 ggtgtataaa taaaggagat aattcaaaat aaaagtttga accatctcct tcttatagaa
    15061 aaacaaaggg gttgatttat atatgaaatt ttatactata cagactttag aattttggaa
    15121 taacaataaa aacaataaat atctatcaaa tgattattct tttatattag aagactttga
    15181 tattccatac aaatggatgt atagtcaaat gacaaataga atatatgaat ttgatgatag
    15241 tatgatatgg gtttggacga agagacctga tttaagatgt agtggttatt tagaaaaagg
    15301 aaaaaaaggt gttttacttg aaatagaaat aagtgaagac aatgtgttat tatcagaatt
    15361 tgatttatgg catttacctt taatggatat tactgtagag ctttatgaag atgaacaaat
    15421 tgaaaagaag gtaagttggg aaagaatatt tgacttagaa ctgtgttcaa aattaaatga
    15481 agttaatata gaagatttga cattgcaagg agtgacatct aaagtgcctc tatctaatgt
    15541 aaaattaata aaagaatttg ttgcaaaata atatttttat tgaaatttat tcaaaaaagt
    15601 tatatattta taacaagagg tgttataaat acataacttt ttaaggggga ttttatatgt
    15661 ctaaacaaca aactggtatt aaatcaacac ttatttcaga aatattggga atttcttatt
    15721 gtattggagc tacattagta aataagtatt tttttaaagg aagtagtatt gaatatcttc
    15781 ctataattat ttgggtatta gctatgggag ctcagtgggt ctataataaa aagtatgatg
    15841 atttaattga tgaggcatca aaatgtatac tatcaagagt taatgatatt gcaataaaag
    15901 tattgttttt ttctgtagct atagtatcta tatttcttgt aactttggac ataaatgtgt
    15961 caaatttaaa tataggaatg tctttattag tcattttgtt tattcaatcc ttgttgaagt
    16021 taattctatt tatatatttt gacagaaagg ggatatacaa ttaggtgtca gtattactaa
    16081 agacaaaaat taaatcctat agagaaaaga accttatgac acaaggtgaa cttgcaaagt
    16141 cagtaggagt aagacgtgaa actattgtac atttagaaaa tggtcgctac aatccttctt
    16201 taaaactggc aatggatatt gctaaggtat ttgatacaac tgtagaagag ttgtttgaat
    16261 ttattgaaga aaataatatt taaatttcat gtattactta tgcattagag aatgaattaa
    16321 ttataggtaa aaaaccaagc ttatagatta gagaataaat gacttataga tgaaaaacca
    16381 agctaatgga ttaaagagtg aatgatttat agattaagaa gtaagcttat agattagaga
    16441 ataaatgact tatagatgaa aaaccaagct aatggattaa agaatgaatg atttatagat
    16501 gaaaaagaaa gcttatagat tagagaataa atgacttata gatgaaaaac caagctcata
    16561 gattaaagaa tgaatgattt atagatgaaa aagaaagctt atagattaga gaatgaatta
    16621 attataggta aaaaacaagc taatagatta aaaaatgaat gatttataga ttaagaagta
    16681 agcttataga ttagagaata aatgacttat agattaagaa gtaagcttat agattagaga
    16741 ataaatgact tataaataaa aaccaagctt atagattaga gaataaatga cttataaata
    16801 aaaaccaagc ttatagatta gagaatgaat taattatagg taaaaaccaa gcttatagat
    16861 taaaaaatga atgatttata gatgaagaac taaacttata gattaaaaaa taaatgactt
    16921 atttatagat gaaaaaccaa acttatagat taaaaaataa atgatttata gatgaagaac
    16981 taaacttata gattaaaaaa taaatgactt atagatgaaa aatcaagctc atagattaaa
    17041 gaataaataa cttattagat gaaaaaccaa gcttatagat aagaagtcca tgcttataag
    17101 ccagaaaatg aatagtttaa tagattaaaa aatacatatt tgtgaattat agaatgaata
    17161 actccataaa ctagaaaatt tataataata tctatatgaa aattagaatt attttatcac
    17221 aaaaaaatta agttaaataa atatgaaatt acttccaaat atacttccaa aaacatgtaa
    17281 tcgcatatat aatataatta taaaaataat aaatattttt atagtagtag ttaattatta
    17341 ataaggaggg gtcaaaatgc ctagcatgat tatggaagtt gtaggagtac tatttatagc
    17401 tattggatta atcttgacat tcattgaaat aaaaggaaat ttcccttctt tttatggttt
    17461 tggtcaaaat taaaatgctg taggtattat agcagcagga attgctttaa ttttctttag
    17521 aaatggcata gatacataga ttctatagct acttatggtg tatttttagc aatagaactt
    17581 gttgtaatag ttgcatattt cttaataaga aaaaaattta ctaaagaaga taaacattta
    17641 agaactaatt aactaaatta tctactataa aattggaaaa ttaaaaacaa ataaaagttt
    17701 gtttttaatt atttaaaatc caaatttgat tatttttaaa gaaactatgt tctgtataaa
    17761 actagaatgt ggtttctttt ttatgtataa aatatttttt agcaaaagtt aaaaggtgtt
    17821 ttataatata aaatagttaa ttgtagatgt taataaaaga aaattatgta tattttagat
    17881 aaagataaga gagtatacta tattttaatt cttttttcaa taaagcttat tgaaaagaac
    17941 tttaacttag aagagtatca ccaaattgca gatacaatat cataagcagt tatttattga
    18001 caaccctttt taataaatat atcatttgaa gtatactgac gaaaaggagt tgaataataa
    18061 tggacatctt attggttgaa gatgataaga atattcgaga aatggtatac gattttctga
    18121 ttagtgagaa ttactctgtt aaaactgcca ttgatggaaa tacagctatt gaaatattta
    18181 aaaattattc atttgatatt attttactgg acttgatgtt accaaagtct agtggttttg
    18241 aagtattaaa atatatacga agtgtgagtg ttgttcctgt tattattact actgcgaaag
    18301 atagtgacac agataaaatg ctagggctta atctaggtgc agatgattat attacaaagc
    18361 ctttttcaat cgtagaattg cttgcaagaa ttaaagctaa tattagacgt gcaacagtgt
    18421 atagcagttc aagaaactct caagatatgg ttattcggta taaggatttg attataaaca
    18481 tatcaaaata tgtagtttca aagaatggaa ttgacttaaa cttaacacat acagaatttg
    18541 aaattgttaa attactagcg actaatcaag gacgtgcttt ttcaaaggaa caattataca
    18601 acgctatttg gaaagaacct tattatggaa atgaaaatgt gctaaatact catattaaca
    18661 ggttaagaaa taaattgtct gatggtatag aaaaaaataa ttatattaaa actctttggg
    18721 gaattggcta caaaatggag gaagaataat ggagttattt ttagttgtaa tttcgattgt
    18781 actatttgct ctctacttaa aagaacgaaa taaatcaaag aaaatggaaa gtgattataa
    18841 gtatatcaat tcaagacttc gtgacataac aaatgatgat gaaattaatt atattttagt
    18901 accatctgat atttcgattg taaaagaaac agcagtagat ataaataaac ttgttgaaaa
    18961 gttttacagc aggcaaatta attataatag aagtgaaaaa gcgatattgc aaatatttac
    19021 aaacatatca cacgatttac gaactcccat cacagtatta aagggttata ttgaaatgct
    19081 atatcttcaa agtcaaaaag aagaattatc ttcttcaatg agagcaattg tggaaaaaat
    19141 gcaaacaaat tcaaatgagc ttgttaattc aataaataac ctgtttaata tggcaaaaat
    19201 ccaatctgga gatatgatat taaatcttca aaaatccaat ctaacacaat tatgtcatga
    19261 gattatttta gagttttatg atttgctaga taaagaaaac tttaatgttg aagttaatat
    19321 tgaagataga cctatatatg caaatgttga cacagaagca ttacaacgta tactcaaaaa
    19381 tttaattgat aatgctataa aatatggttc agatggtaga tttttaggga tttcactata
    19441 tgaaaaggaa aaacatgttt ttatagatat tgaggataaa ggttcaggta tttctgaaac
    19501 tgaaaaagag catattttca caagagctta tactgctgac agaaaaaaag gcaatggttt
    19561 aggtttggct atttctcaag ggcttgcaaa ctctatggga ggttcaattt atgtaaatag
    19621 tagacctaat gtaaaaacag tttttacggt aaaattgaaa agttagaaaa aagttagatt
    19681 taaaagagag aaaagacaat tttattttgt atactgtttt tagaaaggag ggagtatgat
    19741 gaactatatc atagagaccc atcaattaaa gaaaatatat aaagataaag ctgttgttaa
    19801 tgctgttaat attcatgtga aaaagggaga aatttatgga tttgttggac caaacggggc
    19861 aggtaaaagt acagtaatga aaatgttgct aaatcttgtt aatccaaatt ctggagaaat
    19921 agttatgttc ggtaagaaag tggcagaaac tgattttgaa atattaaaaa agattggtac
    19981 tattattgaa aatccgtatt tatatgagaa tttgactgct aaacagaatc ttgatttaca
    20041 ctgtgaatat atgggttatt ataacaaaga acatatatca gaggtattgg aatgtgtcgg
    20101 attgtcaaag caatcaaaca agaaagtttc aaaatattct ttagggatga aacaacgtct
    20161 tgcaatagct agagcaattc ttactaaacc agaattattg attcttgatg aacctataaa
    20221 tgcactcgac ccagagggga taagagaaat gcgagaatta ttccgtaagt taaatacaga
    20281 ttatggaata actatattta tttctagtca tattctttca gaggtagaac aaattgcgga
    20341 cactattgga attattcaag atggtaaact tattaaagaa atttctatgt cagatataca
    20401 caaatatcaa acagattatg ttgaagtaga tgtagataat gtggagcttg ctggatactt
    20461 gttagaaaaa gaatttggaa taaaagactt taaaattact tcggaaagtt gtattgaaat
    20521 atatgattta cgaaaagatg taaaagaaat atccaaaata tttattcaaa atcaaattgg
    20581 tataaatagc attggacgta aacaaagttc tttagaagat tacttcttcc aaactacagg
    20641 tacaggaggt aaagaaacac tatgatacat ttaatgaaac tagagctaaa aaaagttggg
    20701 ctaaaaagat attttttatt ttcaatgctg gggattttct tatgtatgtt ttttgtcttt
    20761 actggattga atgataaatc tacttcaata tatgattatg atgttacatt taggacaatc
    20821 agtttatttt tctgttttta ctatattata ttatttgctg tgttgaatgt ggcatatata
    20881 ataaatgaat atacaaataa aacaatcttg attttattct cttatccact tgaccgtaaa
    20941 aaacttattt tatcaaagtt acttttgatt acgactctta tgatatcatc aatgatagtt
    21001 ggatatatct gttgttttac attcgttgtt gtgttggata agtattttaa tttagttatt
    21061 ggagattttc atatttcaat tttatcttat tggataccag gagcaattaa aagtattatt
    21121 gtattttgct cattaggtat atggacattc gttgttggaa tgataagaaa atcaatacct
    21181 gttacccttg tcagttcaat tatttttatc aatttaagac aatttatttt agctggtaca
    21241 aatacaactc aagacagttt gatttctgtt tttattatag ttggtataac tgtgattggt
    21301 atatattata tactaaatca taaagttact gaaattgact agatactgtg gataggaggg
    21361 tagttattaa ttgtatcttc ttgggataat aatgatttgc tattatatca gctattatta
    21421 atattattag agttttaata atattgcaca accatttgaa gttgagccaa agcttgatga
    21481 attaattgaa tggtactata gtcagagtga agtatctatt aaagttatag ctgaatttca
    21541 ttatagattt gaattgatac atccatttca agatggtaat ggtcgcatag gtagatttgt
    21601 tatgctaaaa cagatgttag aaaataatct tcctataaaa attgtatctt gggatagtga
    21661 ggaattgtat agaaactcct taaattcttg tagtttaggt aattatgtcc ctttaattga
    21721 atatttatca agtttagaag attttagaga agtatataaa atgttatgga aataattgtt
    21781 tg
//
LOCUS       DAEPIN010000064        20943 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.064, whole genome shotgun sequence.
ACCESSION   DAEPIN010000064 DAEPIN010000000
VERSION     DAEPIN010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20943
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.064"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            complement(<1..1486)
                     /locus_tag="KR250_002816"
     CDS             complement(<1..1486)
                     /locus_tag="KR250_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0068732.1"
                     /translation="MTLFRIAMQNVKNSFFNYLMYFISIVFSVFVFFSFKSIEYNEAL
                     SSLGEKTRMSINTSSIVIVVFVFLFIYYSNSFFFNRRRREVGTYSLLGMRKNQIGKIF
                     LYETFLMGIVAILIGIFLGLLFSKLMTMMLVKLMNEMIVVNMNLSIKALVQTLAVFLI
                     IFIVIGIRNTIVIRNKRIIELFNKYPEKYKFKKFIKLKCVLGVLLIAISYLMSISYFI
                     AENIMLSVFILVTIIPGTFLFFSSTMYMIIDAVKKRKSFYYKGQNLIAFSELGFKLKS
                     NSSVLAVIAILIATSVTMLGFTISLYYDIDRNISENYKYSYTINLGNSYVNNEIDKLL
                     YKYKKDNKIIFDKIIELIDKDINIEMYYKNRNSYIEDRTSVDIIRESDFKKLRQYQNN
                     NYEELTSKESVYYVSDSYKKIFFKDFEIEKINLHIKDANEYDINLFKVQKSILEPEIN
                     SQATFDLIVVKDDVFNQLKLHGNSRLIRLIDIKNEKKELDLTLKL"
     gene            complement(1473..2240)
                     /locus_tag="KR250_002817"
     CDS             complement(1473..2240)
                     /locus_tag="KR250_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003722224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0068733.1"
                     /translation="MEEILSVENIKKEYGRKGSKHEALRGITFKVYKGEFVGIMGSSG
                     AGKSTLLNIISTIDLPSSGDIYINGKNTIKMKQNELADFRRDNLGFVFQDSNLLDTLT
                     IKENIMLPLSLKNERVSVIENRIKEISKELNIESILDKYPGEVSGGQKQRGAVCRAIA
                     TKPSLVLADEPTGALDSKSARDLLNCLLKLNKDSNKTILMVTHDAISASFCNRILFIK
                     DGIIFTEIVKGESNREFYNKIVNTVSLIGGVNKNDFI"
     gene            2528..3202
                     /locus_tag="KR250_002818"
     CDS             2528..3202
                     /locus_tag="KR250_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0068734.1"
                     /translation="MFKIMVVEDDVSLKNIIAKCLTKWGHDVHQIENLENIIEEFKNY
                     NPELVLLDINLPFYDGFHWCNEIRKISKVPIIFISSRNSNMDVIMGVNLGADDYIQKP
                     FSVDVLVAKVNALLRRTYNFVDNNSNQIIHNGVTLDLSTATINYEDNTIELTKNEIKI
                     LHELMKYKGQIVSRNKLMKKLWDNDWFVDDNTLTVNVNRIRSKLNEIGLEDFIETKRG
                     LGYIIS"
     gene            3226..4230
                     /locus_tag="KR250_002819"
     CDS             3226..4230
                     /locus_tag="KR250_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBH0068735.1"
                     /translation="MSFKDFLKDKLGFLVYNFTFLIFTVSVIIFSPVDVFLTDTILYI
                     LVVNVVFLFLYLFISYIKKNKFLDKIKNDTFKINMNDIELARCKNEEKIYLNIIKNHQ
                     YQCNNIINNNIEKFEENKEILNMWVHDVKIPIAIIKLILEQNENLIDEKISDDIDSEI
                     FRIENSVEKILYLSRLEDFHKDFLIQEVNIEKIIRNIIKKYSKYFISKKIILDLNNLN
                     FKVLSDEKWLYFIFEQLLSNSLKYSSLGDNISIYCKRTKNYLQLRVEDTGCGIKKEDL
                     NRIFNKGFTGNNGRNNSKSTGLGLYLVKELCDKLGHKIDVDSEYNQYTKFTITFKCDL
                     "
     gene            4342..5571
                     /locus_tag="KR250_002820"
     CDS             4342..5571
                     /locus_tag="KR250_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HBH0068736.1"
                     /translation="MSFLKKFKNKRFRNNSFKSNNFRNSSFGSSGFKSKNKGFKNNPF
                     KNKRFKIYAGIAVIILLILGVLAYVDSKNTELQANENFIETYTIPENEKIFINGMVVP
                     KQTKDFNISGDYELSDVNVTNGQKVNQGDLLFTAKNPTIIAEIDSLKSQLSQYKKQKI
                     SLSDIAENKDAIASINAQITALNSQIASLDKKAYDRVTAPFDGTVYLNDQTGNPDQPV
                     SFMTIQGLEFYMKGQASEQDLPKMKIDQMVNILVFSTDQKLTGRISFISDKPSTPNTE
                     MGAQQNTLSYYDINIAFDNQEGLVNGFHLQASLEVANSSFKIPASCVLKDNKHSYVFK
                     DLDGILKKQIVDVASQNDDFAVVRGGLEQGDIIIKHPTKEMKEGDPVQGDGVTAGSNN
                     GDTTPNKKVEEVTMDVN"
     gene            5579..6253
                     /locus_tag="KR250_002821"
     CDS             5579..6253
                     /locus_tag="KR250_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0068737.1"
                     /translation="MLIKLENIQKYYKVGKDELHVLKSLNLEIESGEFVMIMGKSGSG
                     KTTLLNILGFLDVFDEGRYIFDGTDVTNLSENERSVFRNINIGFVFQQFNLIETLNVY
                     QNVELPLIYNKALKKSNREEIVKDKLSSVGLLDKLKQKPLQLSGGQQQRVAIARCLAN
                     DPQIIFADEPTGALDSETSREIMELLTRLNKQGKTIIMVTHDQDLTKYATKVIRLKDG
                     VFTSEV"
     gene            6256..7488
                     /locus_tag="KR250_002822"
     CDS             6256..7488
                     /locus_tag="KR250_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0068738.1"
                     /translation="MSLLKNSLANLKGHKLRVFVALLWIIIGITSVILVSSIGNGFQK
                     EIKKSVNNVNPNKTTISFESADNTGLTDDMSIFLKPFNAKDLEELSFVEGVERIAPSR
                     DGFNLDSVYSSQASFDKKTTYVDVGPVKKDSKINLICGRDFSLDDEKRKVILLTLQST
                     SEIFENPEDALGHGININGTIFEIIGVLDDSQQNQAGGFFGGYQDMQFTTSLVPKKAF
                     DTLMSQNSYSNEIYQLDLVSSKGYNVNEVANNVIAKLYEMHPGINGSYTTPDPTEQTA
                     YLESINSNVNKYVSIITVVAMFVGGIGVMNIMYVSVMERQREIGIRRAIGAKPRSILF
                     QFLVEAVFITVCGGILGTIVGFAATNYVSKYIGFEAIPSLNSLFYAIVATILTGVVFG
                     LIPAFKASKLDPIKAIYK"
     gene            complement(7774..9039)
                     /locus_tag="KR250_002823"
     CDS             complement(7774..9039)
                     /locus_tag="KR250_002823"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HBH0068739.1"
                     /translation="MILIKNGRVIDPKSQRDENIDLIIKENKIYKIGKFDESDEYEKI
                     IDASGNVVAPGLVDVHVHFRDPGFTYKEDIESGAKSAARGGFTTVICMANTNPIVDNE
                     DTFNYVKEKSKNACINVLQAAAITKGFEGKELVDMEALKKAGVPGFTDDGLPLMDSNL
                     IMEAMIKAKELDVPLSFHEEDPSLVGNPGVNAGKVASELGLKGASSVAEDVMVARDCM
                     LALKTGAKVDIQHISSGVSVDMVRFAKYLGANVVAEASPHHFTLTEEDVLEFGTNAKM
                     NPPLRSKWDRDKIIEGLNDGTIEIIATDHAPHSKEEKDREFIKAPSGIIGLETSLALG
                     ITNLVHKNHLSMMQLIEKMSINPAKLYNLNIGFIEEGAVADIVIFNPEEEWTVESFVS
                     KADNSPFKGKSLYGKVNYTICNGEIVYNA"
     gene            complement(9092..10240)
                     /locus_tag="KR250_002824"
     CDS             complement(9092..10240)
                     /locus_tag="KR250_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HBH0068740.1"
                     /translation="MKSVSVRSCKTYNYEEVKLSIEKNLEDIGGIDKFVKKGSKVLIK
                     PNLLMKKKPEEATTTHPMVVKVVCEKLLELNCDITIADSPGGPYNQSSLKSIYKASGI
                     EEVANELGIKLNYDVSDVKIHSEKAHVLKYMDIIKPIVDSDYIINLCKLKTHIMATFT
                     GGTKNLYGCIAGLKKAEIHYRFPTEELFCENVLLDICDYIKPTLTIMDGIVGMEGDGP
                     SAGTTREIGVLLASQNPYAIDVVACKIISLLPNKVATIRGAIKRGYIQEDFSDIEILG
                     EDIKDLIIKNYKIPNVSKDLRLFSVKLPKFLNEPISKLITPKPVVRFQDCIKCGKCKE
                     ACPASTIEIGTKGAIIDLSKCIRCYCCHELCPKKAIDVKRNFVFKYVK"
     gene            complement(10340..10885)
                     /locus_tag="KR250_002825"
     CDS             complement(10340..10885)
                     /locus_tag="KR250_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH peroxidase"
                     /protein_id="HBH0068741.1"
                     /translation="MKKFVCTVCGYIHEGDAAPAQCPVCKVGADKFEEMKGEMVWADE
                     HRIGVAQGVDAEIIEGLRANFTGECTEVGMYLAMSRQADREGYPEVAEAYKRIAFEEA
                     EHAAKFAELLGEVVVADTKENLRVRVDAEYGATDGKLKLAKRAKELGLDAIHDTVHEM
                     CKDEARHGKAFLGLLNRHFGK"
     gene            complement(11108..12052)
                     /locus_tag="KR250_002826"
     CDS             complement(11108..12052)
                     /locus_tag="KR250_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBH0068742.1"
                     /translation="MQKNKKEGALLKKYFGEIGLIFIAIIWGSGFVATQFALDGGLTP
                     LQIITLRFFLAAIIMNLLFFKQIRANMGKKLLKAGGILGIFLFLAFTVQTIGLMYTTP
                     SKNAFITAANVVIVPFIGFILYRRKLDKIGIISSLVALIGIGILSLEADFSINFGDFL
                     TLICSFGFAFHIFFTSEFAKDNNPMALTAIQFTVAFLMSVVVQTFAGQLKMEAELSGY
                     MGTMYLAVFSTTIGFLFQTICQKRVDGTRTAIILSTEAVFGTIFSIIILKELITAKLV
                     IGSILIFVAIITAETKLSFLKSKKVKLKDSEESSLESI"
     gene            12438..13319
                     /locus_tag="KR250_002827"
     CDS             12438..13319
                     /locus_tag="KR250_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HBH0068743.1"
                     /translation="MVKKRKKKTVTLTILVVIVIGIFSMAWADMSRKKEMKETQERLK
                     AERLEKERIEKEKNEPIIGAKKEAVKEYGYDAKIVWDKLNKYDYSNNGEKIVFLTFDD
                     GPSTTNTPQVLDILKRHGVRGTFFIKGDSLERKGANEILKRTFDEGNAIAHHSYTHDY
                     KKLYPNRSLNLDTFVNELNKTDEAMKKVLGKNFSSNVVRCPGGYMSWKNMEPLGNYLK
                     EKNMASIDWNALNADAEGKKKNAQELFEHAKKSSEGKEMVVLLMHDTYGKQETVNALD
                     QIITYFKDNGYQFKILI"
     gene            complement(13380..14588)
                     /locus_tag="KR250_002828"
     CDS             complement(13380..14588)
                     /locus_tag="KR250_002828"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HBH0068744.1"
                     /translation="MKSIDEQMRIIMKGVDDLIDEKELREKLIKSEKEGKPMIVKLGL
                     DPSAPDIHLGHTVVLRKMKQLQDLGHQIVIIIGDFTGKIGDPTGKSKARKALTTEQVL
                     ANAKTYEEQIFKVLDKEKTIVRFNSEWLAKLNFEDVIKLAATITVARMLEREDFKKRY
                     EGQMPISVHEFFYPLMQAYDSIALEADIELGGTDQRFNLLMGRSLQREFGMESQIVIM
                     MPLIEGLDGKEKMSKSLGNYIGIDEEAGIMYQKSMEIPDELIIKYYNLVTDVHPDEVN
                     KIESQLKEGSVNPRDIKMNLAREIVTLYHGEESAKEAEERFKSVFQKGQIPEDIQTIQ
                     VKEDGFDLIEVLVSNEIVKSKSEVRRLASQGGVKVNGEKVEDLSTIVKESELVVQIGK
                     KKFVKIELVK"
     misc_binding    complement(14661..14913)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(15065..16054)
                     /locus_tag="KR250_002829"
     CDS             complement(15065..16054)
                     /locus_tag="KR250_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HBH0068745.1"
                     /translation="MIYGGSVLNTTKKLTEAALLSSLFIVVTIIAVSTGFGYAIYLDF
                     IVPVFFCIICLKCDLKYTILSGITSLLIISLVLGNLGTAIWASQSVLLGIMCGYLINK
                     PTMVMDDLVYGSVFGVIVMVFIDIYASTLIGYSFMKEFQGYSKWIYFNGYTNFIYYLM
                     IALFPFGMVFCIYLLSLILGNKLHILDSNSLKKFLIFKNFRNLNQFLCCSKKAFYICV
                     SYLAIFEVLKLLNVKVDSVYLKTIFISITYISVYFIIRDSCMSLQNFIIAKFRKLLYA
                     RILFLIIILLLFFIFDVTVISLIIINAFLNKKINIRLSQSNIVNKQINLLIEK"
     gene            16206..16646
                     /locus_tag="KR250_002830"
     CDS             16206..16646
                     /locus_tag="KR250_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBH0068746.1"
                     /translation="MFKIKHFNDLSLDEFYEIAKSRYEVFACEQKIFSLNDYDDIDKS
                     SYHIFLKENGLICAYARIIPKEYSSYNDVSIGRVLVLSSHRRKGLAKQMMDCAIDFIK
                     VNLHENNITLSAQTYIKNLYLSCGFKEISEVYDEAGIEHIKMRL"
     gene            16973..17200
                     /locus_tag="KR250_002831"
     CDS             16973..17200
                     /locus_tag="KR250_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBH0068747.1"
                     /translation="MKNLNDIQVKKTVKVEDILSSGNLRERMLALGLTRGAVIDVVRK
                     GPKNNLTVYKIRGSKIALRQEESSLILVSDI"
     gene            17226..19346
                     /gene="feoB"
                     /locus_tag="KR250_002832"
     CDS             17226..19346
                     /gene="feoB"
                     /locus_tag="KR250_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HBH0068748.1"
                     /translation="MGLTHNSTKMSSLKDMFDIDNKEDQFVIALAGNPNTGKSTVFNH
                     LTGLRQHTGNWPGKTVATARGNFKYKNTEYALIDLPGTYSLFALSQEEIVARDFICFG
                     NPDAVIVVCDATCLERNLNLVFQVMELTDKVILCINLIDEARKKGITIDKKLLEDSLG
                     IPVILTAARNGSGMDELLDTLNDVSFDKYKLNNKPVRYNENIENVVKSIQPELDNIIP
                     GINSRWLGLRLIDGDESIFESMSNYIDKDSIDAINEVKKKIPDNINKQKIRDEFTKIN
                     YDYAKKLSDECCSNVAKKSTDREEKVDKILTSKIFGLPIMLLLLGTILWITIEGANYP
                     STLLSNLLLGFEPSISGILNSINCPSWLNDMLVLGLYRTLAWVISVMLPPMAIFFPLF
                     TLLEDFGYLPRVAFNLDHLFKKACAHGKQCLTMCMGFGCNAAGVIGCRIIDSPRERLI
                     AILTNNFVPCNGRFPTLIAISTIFFSSVITNSFVSSVATALCITLLIILGVIITLLVS
                     YTLSKTLLKGVPSTFTLELPPYRVPQIGRTLYTSIIDRTIFVLGRAVMVAIPAGVITW
                     IFANIYIGDLSILSHVANFLDPLAKLIGLDGFILLAFILGFPANEIVVPILLMAYLAT
                     GSMIELDSFSALGQVLREHGWTYLTALNVMLFSLLHWPCATTLLTIKKETGSLKWTAL
                     GFLMPTILAFVVCFLTTTVYNLFI"
     gene            complement(19479..20297)
                     /locus_tag="KR250_002833"
     CDS             complement(19479..20297)
                     /locus_tag="KR250_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBH0068749.1"
                     /translation="MEKQYFTTGEFAKLCGISKQTLIFYDKIGIFSPEYKDKNNYRYY
                     SVYQYDTLDILQSLREIGMSLEEIKEYIQNRTPQLCVKMLKEEEKKIREKIKKLRKIS
                     SKMQNRINITVEGISKMNSEEIYISKKFEEYLVLSSDLSNMSNSDFMMELIDFTNYCK
                     SKNLYQGYELGVIVSNENIKNGDYLSISSFYLKIDKKIKDKKLYVKPEGMYACIKHIG
                     KYEDSYKSYEKLKKYIYENEYKIVGNSYEESILDFFCESNEDNYMTEISIQVSR"
     gene            20504..20788
                     /locus_tag="KR250_002834"
     CDS             20504..20788
                     /locus_tag="KR250_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0068750.1"
                     /translation="MLVRVKIVIGATIRYLIKHKVNYCNKKSLDKLELKYGNIDREKA
                     VKLEIFYQYLIELEYIEMSLLIRRLDTAITSLALVSIITIASYYLIRKNV"
BASE COUNT         7443 a         3118 c         2636 g         7746 t
ORIGIN      
        1 ttaattttaa agtcaaatcc agttcttttt tttcattttt tatatcaatt aatcttatta
       61 atctagaatt tccatgtaat tttaactgat taaatacgtc atcttttact acaattaaat
      121 caaacgtagc ttgtgaattt atttctggct ccaagatgct tttttgtact ttaaacagat
      181 ttatatcata ctcatttgca tctttaatat gcaagtttat tttttcaatc tcaaagtctt
      241 taaaaaatat ttttttgtat gaatcagaaa catagtacac agattccttt gaagttaatt
      301 cttcataatt gttattctga tattgtctta attttttaaa atcactttct ctaataatat
      361 ctacagacgt tctatcttct atataactat ttcgattttt ataatacatt tcaatattta
      421 tatctttgtc tataagttct attattttat caaagattat cttattatct tttttatatt
      481 tatataataa tttgtcaatt tcgttattta cataagaatt tcctaaattt atggtataac
      541 tgtatttata attttcactt atgttcctat ctatatcgta ataaagtgat atggtaaacc
      601 ctagcatagt tacacttgta gctattaata ttgctattac agccaataca ctactattgc
      661 tctttaattt aaatccaagt tcagaaaatg caattaaatt ctgtccctta taataaaaac
      721 tttttctctt cttaacagca tcaataatca tatacatagt tgatgaaaaa aataaaaaag
      781 ttccaggtat tattgtaact aatataaaaa ctgaaagcat aatattttca gcaataaaat
      841 aactaattga cattaaataa gaaatagcta ttaataaaac accaagtaca cattttaact
      901 ttataaattt tttaaactta tacttttctg gatatttatt aaataactct ataattctct
      961 tatttctgat aactatagta tttcttattc caataactat gaatattatt aaaaataccg
     1021 ctagtgtctg aactaaggcc tttatactta agttcatatt tactactatc atttcattca
     1081 ttaattttac aagcatcata gtcataagct ttgaaaataa cagtcctaaa aatataccta
     1141 ttagtattgc aactataccc atcaaaaaag tttcatataa aaatattttt cctatctgat
     1201 ttttcctcat tccaagtaaa ctataagttc ctacttctcg cctacgtcta ttgaaaaaaa
     1261 atgagtttga ataatatata aataaaaata caaataccac tattacaatt gaacttgtat
     1321 taatactcat cctagttttc tctccaagtg aactcaacgc ttcattatac tctatagatt
     1381 taaaactaaa aaatacaaat acactaaata caattgaaat aaagtacatt aaataattaa
     1441 aaaaactatt ctttacattt tgcattgcaa ttctaaataa agtcattttt attaactcct
     1501 ccaattaagg aaactgtatt tactatttta ttatagaact ctctgttact ttctcccttt
     1561 acaatttctg tgaatattat tccatccttt ataaagagta ttctattgca aaaactcgca
     1621 cttatagcat catgagttac catcaatatt gttttattgc tgtctttgtt taattttaat
     1681 agacaattca acaaatccct agcagattta gaatctaatg ctcctgtagg ctcatctgca
     1741 agtactaatg atggttttgt tgctatagct ctacatacag ctcctctttg cttttgtcca
     1801 ccagaaactt cgccagggta tttatctaat atagattcta tatttaattc ttttgaaatc
     1861 tcttttattc tattttctat tactgatact ctttcatttt ttaaagaaag aggcaacatt
     1921 atattttctt ttattgtaag tgtatccaaa agattagaat cttgaaatac aaagcctaga
     1981 ttatcacgtc taaaatctgc caattcattt tgcttcattt taatagtatt ctttccattt
     2041 atataaatat ctcctgatga aggtaaatct attgtagaaa taatatttaa aagtgttgat
     2101 ttaccagcac cggaagaccc cataatacca acaaattctc ctttataaac cttgaaagtt
     2161 attcctctta gagcttcatg ttttgaacct tttcttccat actccttttt tatattttca
     2221 acagataata tttcttccat agttaataat cacctttcat cacaattagt tatttatcaa
     2281 ttctcacaaa tatattatat atattaaaat ggcttttaaa catttaatta tattaattta
     2341 aggttacatt ttcgtaagta ttaattatat ttcttcaaat ttattgcaat tatgtttaaa
     2401 atataattta tcaagtaaaa tatagttata ttaatataat tattttaggt aataaggtag
     2461 aaatatttta atttatcaag tagaatatag ttatgttaat ataactattt tgaattagga
     2521 ggtaagaatg tttaaaataa tggtagttga agatgacgtc tcattgaaaa atatcatagc
     2581 aaagtgtctt actaaatggg gacatgatgt tcatcaaata gaaaatcttg aaaatataat
     2641 agaagagttc aaaaactata acccagaatt agttttattg gacataaacc ttccatttta
     2701 tgatggtttt cattggtgta atgaaataag aaaaatttca aaagttccta taatttttat
     2761 ttcttcaaga aattctaata tggatgtaat aatgggtgta aatttaggtg cagatgatta
     2821 tatacaaaaa cctttttctg tagatgtctt agtagctaaa gttaatgccc ttttaagaag
     2881 aacttataat tttgttgata ataactcaaa tcagattatt cacaatggtg ttactttaga
     2941 tttatcaact gcaacaataa actatgaaga taatactatt gagctaacaa aaaatgaaat
     3001 caaaattctc catgagctta tgaaatataa ggggcagata gtaagtagga ataaactcat
     3061 gaaaaaatta tgggataatg attggtttgt agatgataat acattaactg tcaatgtaaa
     3121 tagaatacgc tcaaaattaa atgaaattgg tttagaagat tttatagaaa caaaaagagg
     3181 attgggttat ataatcagtt aaaacaattc taagtaggtg aatttatgag ctttaaagat
     3241 tttctcaagg ataaactagg ttttttagta tacaatttta ctttcttaat ttttacagta
     3301 tcagttatta tattttctcc tgtagatgtt tttctgactg atactatttt atatatatta
     3361 gttgtaaatg ttgtgttttt atttttatat ctatttatta gctacatcaa gaagaacaaa
     3421 tttttagata agataaaaaa tgatacattc aaaattaata tgaatgatat agaattagct
     3481 agatgtaaaa atgaagaaaa aatatacttg aacattatta aaaatcatca atatcaatgt
     3541 aataacataa taaataataa tatcgagaaa tttgaagaaa acaaagagat tttaaatatg
     3601 tgggtacatg atgtaaagat acctatagct ataataaaac tcattcttga acaaaatgaa
     3661 aatttaattg atgaaaaaat ttcagatgac atagatagtg aaatatttag aattgaaaac
     3721 tccgttgaaa aaattttata tttatcaaga ctcgaagatt ttcataagga ttttttaatt
     3781 caagaggtaa atattgaaaa aatcattaga aatattataa aaaaatactc aaaatatttt
     3841 atttcaaaaa aaattatttt agacttaaat aatttgaatt ttaaggtttt atctgatgaa
     3901 aaatggctat acttcatatt cgagcaatta ttgagtaatt ccctaaaata ctcctcatta
     3961 ggagataata tatctatcta ttgcaaaaga accaaaaatt atttacaatt aagagtggaa
     4021 gatactggtt gtggtataaa aaaggaagat ttaaatcgaa tattcaataa gggatttaca
     4081 ggtaataatg gaagaaacaa ttctaaatct acaggtcttg ggctttattt agtaaaagaa
     4141 ctttgtgata agcttggtca taaaattgat gtagattcag agtacaacca atatacaaaa
     4201 tttactatta cattcaaatg tgatttatag tatcttatta ttgatagttt attttcatta
     4261 acttatattc aacttcttat agtaattcta tatgtacatc ccagtatata attaagtagt
     4321 ttttataaaa aggaggaaga catgtctttt ctaaaaaaat ttaaaaataa aagatttaga
     4381 aataacagtt ttaaaagtaa caactttaga aatagcagtt ttggaagtag tggcttcaag
     4441 tctaaaaata aaggttttaa aaacaatccc tttaaaaata agagattcaa aatatatgca
     4501 ggaatagcag taatcatact tttaattcta ggagttcttg cttatgttga ttcgaaaaac
     4561 actgaattac aagctaatga aaattttatc gaaacatata ctattcctga aaatgaaaaa
     4621 atatttataa atgggatggt tgttcctaag caaactaagg acttcaatat atctggagat
     4681 tatgagttaa gtgatgttaa tgttacaaat ggtcaaaaag taaatcaagg agaccttctt
     4741 tttactgcta aaaatccaac tataatagca gaaatagata gtttaaaatc acaacttagt
     4801 caatacaaaa aacaaaaaat atcactttct gatattgctg aaaataaaga tgctattgct
     4861 tctataaatg cacagataac tgctttaaac tctcagatag cttctctgga taaaaaagct
     4921 tatgacagag ttactgctcc ttttgatggc acagtatact tgaatgacca aactggaaat
     4981 ccagaccaac cagtttcatt tatgactatt caaggtctag agttttatat gaaaggacaa
     5041 gcaagtgaac aagatttacc taaaatgaag attgaccaaa tggtcaatat actagttttt
     5101 tcaactgacc aaaagcttac tggtagaata agttttatat ctgataaacc atctactcca
     5161 aatactgaaa tgggagctca gcagaataca ctatcttatt atgatataaa catagcattt
     5221 gataatcaag aaggattagt aaatggattc catcttcaag catcacttga agtagctaat
     5281 tcaagcttta aaataccagc atcttgtgta cttaaagaca acaaacactc atatgtattt
     5341 aaggatttag atggtatatt gaaaaagcaa attgtagatg tagcaagtca aaatgatgat
     5401 tttgctgtag ttagaggcgg tcttgaacaa ggcgatataa taataaaaca ccctactaaa
     5461 gaaatgaaag aaggagaccc agttcaaggc gatggcgtaa ctgctggttc taataatgga
     5521 gatactactc caaacaaaaa agtagaagaa gttactatgg atgtgaatta atttacatat
     5581 gttaattaaa ttagaaaata ttcaaaaata ttacaaagta gggaaagatg aattacatgt
     5641 tttaaaatcc ttaaatttag aaattgaatc tggagaattt gtcatgataa tgggtaagtc
     5701 tggtagtggt aaaactactt tacttaatat cttaggtttt ctagatgtat ttgatgaagg
     5761 aagatatata tttgatggaa cagatgtaac taatttgagt gaaaatgaac gttctgtttt
     5821 tagaaatatt aatattggat ttgttttcca acaatttaat ttaatagaaa ctctaaatgt
     5881 atatcaaaat gtagagttgc ctctaattta caataaagca ctaaaaaaat ctaatagaga
     5941 agaaattgtt aaagataaat taagttctgt tgggcttttg gacaagctta agcagaaacc
     6001 acttcaatta tctggtggtc aacaacagcg tgtagccatt gcacgttgcc tagcaaatga
     6061 ccctcagata atctttgctg acgaaccgac tggggcatta gatagtgaaa ctagcagaga
     6121 aattatggag cttttaacaa ggctcaacaa acaaggtaag actataataa tggttacaca
     6181 tgatcaagat ttaactaagt atgcaactaa ggttatacga cttaaagatg gtgtgtttac
     6241 ttcggaggtg taatcatgag tttattaaaa aattctttgg ccaatttaaa aggtcacaag
     6301 cttcgtgtat ttgtagcttt actctggatt ataataggaa taacttcagt aatacttgtt
     6361 agttctatag gtaatggatt tcaaaaagaa attaagaaat ctgtaaacaa tgtaaaccct
     6421 aataaaacta ctatttcgtt tgaatcagcc gataacacag gtcttactga tgatatgagt
     6481 atttttttaa aaccctttaa tgcaaaagat ttagaagaat tatcatttgt tgaaggagta
     6541 gaaagaatag ctccttcaag agatgggttt aaccttgatt cagtttactc ttcacaagca
     6601 tcttttgata aaaaaactac gtatgttgat gttggaccag taaaaaaaga ttctaaaata
     6661 aatttaattt gtggtagaga tttttcttta gatgatgaaa aaagaaaagt aattttactt
     6721 acattacaaa gtacaagtga aatttttgaa aatccagaag atgccttagg tcatggtatc
     6781 aacataaatg gaactatatt tgaaataata ggggtattag atgattctca gcaaaaccaa
     6841 gctggaggat tctttggagg ttaccaagat atgcaattta ctacttccct agtacctaaa
     6901 aaggcttttg acactttaat gagtcaaaat tcttattcaa atgaaattta ccagttagac
     6961 ttagtttctt ctaaaggcta taatgtaaat gaagttgcaa acaatgttat agctaaatta
     7021 tatgagatgc accctggtat caatggttct tatactacac cagacccaac tgaacagaca
     7081 gcttatcttg aaagcattaa ttccaatgta aacaaatatg tatctataat aactgtagtt
     7141 gcaatgtttg ttggtggtat tggtgtaatg aatataatgt atgtatctgt tatggaaaga
     7201 caaagagaaa ttggtataag acgtgctatt ggagcaaaac ctaggtctat attatttcaa
     7261 tttttagttg aagctgtttt tataacagta tgtggtggaa ttttaggaac tatagttgga
     7321 tttgctgcta ctaactatgt ttctaaatat ataggttttg aagctatacc tagcttaaat
     7381 agcttatttt atgcaattgt agctactata ttgactggag ttgtatttgg tctgattcca
     7441 gcctttaaag cttctaaact agaccctata aaggcaattt ataagtagaa attgtataaa
     7501 gattttcgat ttttagatta gtttatataa aacatactta aatatataac gcactattat
     7561 atatgataga ttaggaactt aatctttcca aatataatag tgcgttcttt cataaaaagg
     7621 attctcttcc ttactatatc taaattacca agatgttatg ttctaattgt tcaaactaat
     7681 acttccaatt ttgattaatc aatctcattc tcctaatata caatctttta ctttcaatca
     7741 ttgtacactc cgccttaaaa aaataatctt aaattaggcg ttatatacaa tctccccatt
     7801 acatattgta taattaacct ttccatataa agatttacct ttaaatggtg aattatcagc
     7861 tttagaaaca aagctttcaa ctgtccattc ttcttctgga ttgaatataa caatgtctgc
     7921 tacagcccct tcttctataa atccaatatt taaattgtac aattttgcag gatttataga
     7981 cattttttct attaactgca tcatactaag atgattttta tgcaccaaat ttgttattcc
     8041 aagagctaaa gaagtttcaa gcccaataat tccacttgga gcttttataa actctctatc
     8101 tttttcttct ttactatgag gtgcatggtc tgttgctatt atttcaatag taccatcatt
     8161 taatccctct ataatcttat ctctgtccca tttacttctt agaggtggat tcatttttgc
     8221 atttgttcca aactccaata catcttcctc agtcaatgta aagtgatgtg gtgaagcttc
     8281 tgcaactaca tttgcaccta agtattttgc aaatctaacc atatcaacag atacacctga
     8341 acttatatgt tgtatatcaa ctttagcacc agtctttagt gccaacatac aatctctggc
     8401 taccataaca tcttctgcta cacttgatgc accttttaat ccaagttctg aagctacctt
     8461 tcctgcattt acacctggat ttccaactaa agaagggtct tcttcatgaa aactaagtgg
     8521 tacatccaac tcttttgcct taatcattgc ctccataatt agattgctat ccattaaagg
     8581 taatccatca tcagtaaacc ctggtacacc agctttttta agagcttcca tatcaactaa
     8641 ctctttacct tcaaaacctt tagttatagc tgctgcttgt aaaacattta tacatgcatt
     8701 ttttgacttt tctttaacat aattaaaagt atcttcatta tctacaatag gattagtatt
     8761 tgccatacat ataacagttg taaatccacc tctagcagca gatttagctc cactttctat
     8821 atcttcttta tatgtaaacc cagggtctct aaagtgtaca tgtacatcca ctaaacctgg
     8881 agcaactaca ttaccactag catctattat tttttcatat tcatcacttt catcaaactt
     8941 gcctatttta taaattttat tttcttttat aattaagtca atattctcat ctctttgact
     9001 tttagggtct attactctac catttttaat taaaatcata ttatttctcc tttatatttt
     9061 taatttatat agactcatat aatattatac attattttac atacttaaat acaaaattac
     9121 gtttgacatc aatagccttt ttaggacaaa gttcatggca acagtaacat cttatacatt
     9181 tacttaagtc tataatagca cccttagtcc ctatctctat agtggatgca ggacaagctt
     9241 ccttgcattt tccacatttt atgcaatctt gaaatcttac cacaggcttt ggtgttataa
     9301 gctttgaaat aggttcattt aaaaattttg gtagctttac ggaaaacaat cttaaatctt
     9361 tacttacatt tggaatcttg taatttttta taattaaatc ttttatatct tcacctaaaa
     9421 tttcaatatc tgaaaaatct tcttgtatat atcctctctt aatagcacct ctaattgtag
     9481 caactttatt tggaagtaaa cttataatct tacatgcaac aacatctata gcatatggat
     9541 tttgtgatgc taataaaact cctatttctc gagtagtacc agctgaaggt ccatcacctt
     9601 ccatacctac tataccatcc ataattgtca atgttggttt tatataatca caaatatcta
     9661 aaagtacatt ttcgcaaaac aactcttctg taggaaatct ataatgtatc tctgcttttt
     9721 tcaatccagc tatgcatcca tataagtttt ttgtaccacc agtaaaagtt gccattatat
     9781 gagtctttaa tttacaaaga tttattatat aatctgaatc tactattggt ttaataatat
     9841 ccatatattt tagaacatgt gctttttcac tatgtatctt aacatctgaa acatcataat
     9901 ttaacttaat gcccagttca tttgctactt cttcaattcc agatgcctta taaatgctct
     9961 taagagaact ttgattatat ggaccaccag ggctatctgc tattgtaata tcacaattta
    10021 actctaaaag tttttcacat acaactttta caaccatagg atgtgtagtt gtagcttctt
    10081 caggcttttt tttcattaat aaatttggtt ttataagtac tttgcttcct tttttaacaa
    10141 acttatctat accaccaata tcctctaaat ttttttcaat agataacttt acttcttcat
    10201 aattgtaagt tttacaactc ctaacagata ccgacttcat attatctcca tccttctatc
    10261 acaacagttt atttatataa tttaaaaaag gagaaaccaa agtttctcct tttaatctat
    10321 gttttaattg ttttaattat tattttccaa aatgtctgtt taataatcct aagaatgctt
    10381 taccatgtct agcttcgtct ttacacattt catgtactgt atcatgtata gcatctaatc
    10441 ctaattcttt agctctctta gctaatttta attttccatc agttgcacca tactcagcat
    10501 caactctaac tcttaagttt tcttttgtat ctgcaactac aacttctcca agaagttctg
    10561 caaatttagc agcatgttca gcctcttcaa aagctattct cttatacgct tcagctactt
    10621 ctggataacc ttctctatca gcttgtctac tcattgctaa atacattcct acttctgtac
    10681 actcaccagt aaagttagct cttaatcctt cgattatttc tgcatctaca ccttgagcta
    10741 ctcctattct atgttcatca gcccaaacca tttcgccttt catttcttca aatttatcag
    10801 ctccaacttt acatactgga cattgtgctg gtgcagcatc tccttcatgt atatatccac
    10861 atactgtaca aacaaatttt ttcataatta atttcctccc aattttatat aattaaattt
    10921 ttatttttaa gtttaataac ttgctacaag aatatatata ccccagactt tatagcttaa
    10981 acactaaaaa taaaatttat ttcctaatta attttatcac tttgaaacac cagtatcaac
    11041 caagttttga tgtcaaattt ctacataata cataatttat ttgattaatt aggaaatagt
    11101 tgtcatatta aatactttct aatgaagact cctctgaatc tttaagtttc acttttttgc
    11161 tctttaaaaa agataattta gtttctgcag ttatgattgc aacaaatatc aagatactac
    11221 caataaccaa ttttgctgta ataagctcct ttaaaattat tatagaaaat attgtaccaa
    11281 atacagcctc tgtggataat attatagctg ttctagttcc atcaactctt ttttgacata
    11341 tagtctgaaa taaaaatcca attgtagtac taaatacagc tagatacata gttcccatgt
    11401 aaccagataa ttcagcttcc atctttaatt gacctgcaaa tgtttgaact acaacagaca
    11461 ttaaaaacgc tactgtaaat tgaatagctg tcaatgccat tgggttgtta tcttttgcaa
    11521 actcacttgt aaagaatata tgaaatgcaa atccgaatga acatataagt gttagaaaat
    11581 ctccaaaatt aatagagaaa tcagcttcta aagaaagtat cccaattcct ataagagcta
    11641 ccaaactact tattattcct attttatcaa gttttcttct ataaagtata aatcctatga
    11701 atggaactat tacaacatta gctgctgtta taaacgcatt tttagatggt gtagtgtaca
    11761 taagtccaat agtttgtact gtgaatgcta gaaataaaaa tattcctaaa atccctcctg
    11821 cttttaataa ttttttaccc atattggcac gaatctgttt aaaaaacagt aaattcataa
    11881 ttatagctgc caagaaaaat cttaaagtta tgatttgtag aggtgtaagc ccaccgtcta
    11941 atgcaaattg ggttgctaca aagccacttc cccatataat tgctataaat attagtccta
    12001 tttctccaaa gtattttttc aataaagctc cctccttttt atttttttgc aaaattataa
    12061 accccctaaa cagcaagaga gtttttctat aagcattact ataaattaat atagtaaatt
    12121 ttttcagtat tttaaatcta aatttacata cattttgagc tcaaatttat atccactatt
    12181 taacagtaat atattaatca ttttttgtaa acaccttttt aataaatttt tatatttttt
    12241 atattttttt acctatacta aattttttta tagatatttt taatatatat gatactttaa
    12301 tattatgcaa ataatgtcat ataagttaca ctttagttat cttagttaca actttgtcgg
    12361 tttacttttt ctactttctt cgatataatt ataatatatt tttgatacaa ttcgacaaaa
    12421 gggggaatgc tgtaattatg gtaaaaaaaa gaaaaaagaa aactgttaca cttactatac
    12481 ttgttgtaat agttattggt attttttcta tggcatgggc ggacatgtct agaaaaaaag
    12541 aaatgaaaga aactcaagaa agacttaaag ctgaaagatt agaaaaagaa agaattgaaa
    12601 aagaaaaaaa tgaacctatt attggtgcaa aaaaagaagc tgtaaaagaa tatggatatg
    12661 acgcaaaaat agtttgggac aaacttaaca aatacgacta ttcaaacaat ggagaaaaga
    12721 tagtctttct tacatttgat gatggaccat ctactactaa tactccacaa gttctagata
    12781 tcttgaaaag acatggtgtt agaggaactt tctttattaa gggtgattct cttgaaagaa
    12841 aaggagctaa tgaaatttta aaaagaacat ttgacgaagg aaatgcaata gcccatcact
    12901 cttatactca tgactataaa aaactatatc ctaacagaag cctaaatctc gatacattcg
    12961 taaatgaact taacaaaact gatgaagcta tgaaaaaagt cttaggaaag aatttttcct
    13021 caaatgtagt tagatgccca ggcgggtaca tgtcatggaa aaatatggaa cctctaggaa
    13081 actacttaaa agagaaaaac atggcatcaa ttgattggaa cgctttaaat gctgatgcag
    13141 aaggtaaaaa gaaaaatgca caagaattat ttgaacatgc aaagaaatct tctgaaggca
    13201 aagaaatggt tgttctttta atgcatgata cttatggaaa acaagaaaca gtaaatgcat
    13261 tggaccaaat tataacatat tttaaagaca atggttatca atttaaaatt ttaatataat
    13321 aaaaaagcaa gtttattgaa tgtacatttt atactcaata gacttgcttt tactttactc
    13381 tattttacta gctcgatttt tacaaatttt ttctttccta tttgaacaac taattcactc
    13441 tcttttacaa tagtgcttaa atcctctact ttttcaccat ttacctttac tccaccttga
    13501 gaagctaatc ttctaacttc actcttactc ttaacaatct cattagaaac tagtacttcg
    13561 attaagtcaa atccatcttc cttaacttga attgtttgta tatcttcagg tatttgacct
    13621 ttttggaata cagatttaaa tctttcttca gcttctttag cagattcttc tccatggtat
    13681 aatgttacta tttctcttgc taagttcatc tttatatctc ttggatttac tgaaccctct
    13741 ttcaactgag attctatttt attaacttca tcaggatgaa catctgtcac taaattgtag
    13801 tatttaatta taagttcatc aggaatttcc atagattttt ggtacattat tcccgcttct
    13861 tcatctattc caatatagtt acctaaactc ttactcattt tttctttacc atctaatcct
    13921 tctattaaag gcatcattat tactatttga gactccatac caaattctct ttgtaatgat
    13981 cttcccatta gtaaattgaa tctttggtca gttccaccaa gttctatatc agcttctaat
    14041 gctatagagt catatgcttg cattaatggg tagaagaact catgtactga tattggcatt
    14101 tgtccttcat atctcttttt aaaatcttct ctttctaaca ttctcgcaac agtaatagtt
    14161 gctgctaatt ttattacatc ttcaaaattt aacttagcta accactcgct attaaatctt
    14221 acaatagttt tttctttatc taaaactttg aaaatttgtt cttcataagt ttttgcattt
    14281 gcaagaactt gctctgttgt taaagctttt ctagctttag attttccagt tgggtctcca
    14341 atttttcctg taaaatctcc aattattatt actatttgat gacctaaatc ttgtaattgt
    14401 ttcatctttc taagaactac tgtatgccct aaatgtatat ctggagcact tgggtctaga
    14461 cccaatttta ctatcatagg ctttccttct ttttctgatt taataagctt ttcccttaac
    14521 tctttctcat ctattaaatc atctacacct ttcataatta ttcgcatttg ctcgtctatc
    14581 gatttcattt ttcattcctc ctaaaatatt tatataatct ataattttta tttttataat
    14641 aaaaaaagct ttcatcctct taaaaggacg aaagcattgc ttacgtgtta ccaccttagt
    14701 tcatcaatat ctcacgatat taatctctta gagtacaatt acatatactc gtgtactata
    14761 acgtgtacta atccgatatc tcttaatggt tgttatcttt cagagatagt gctctgagat
    14821 gtattcaaac taaatcaatt tattccattt caccaaactg gaactctctg ttaaataaaa
    14881 tctagcccta cttcttctct tcatagcatt tatatattca gattatttca tcaattacaa
    14941 gaaatttttc ttaacttact cataatatat tattattttt atattttgtc aacctttttt
    15001 attattatat acatgttttt ttacattata ctactttttt atatttttta ccaataccca
    15061 aaatttattt ttcaatcaaa agatttattt gtttattgac tatattactc tgactcaatc
    15121 ttatatttat tttcttattt aaaaatgcgt ttataattat taaactaata actgttacat
    15181 caaatataaa aaacagcaat aaaattatta tcaaaaataa aattctagca tataataatt
    15241 ttctaaactt agctattata aagttttgta gtgacataca tgagtctcta atgataaaat
    15301 agacacttat ataggttatt gatataaaga ttgtttttaa ataaacagaa tcaactttaa
    15361 cattcaacag ttttagaact tcaaaaatgg ctagataaga aacacatatg taaaaagctt
    15421 tttttgagca acataaaaat tggtttaaat ttctaaagtt cttaaatatt aaaaattttt
    15481 ttaaagaatt agaatctaaa atatgcaatt tattccctaa aattaaactt aataaatata
    15541 tgcaaaacac cataccaaaa ggaaacaatg ctatcattaa ataataaata aaatttgtat
    15601 acccattaaa ataaatccat ttgctatatc cttgaaattc tttcataaaa ctataaccta
    15661 ttaatgttga agcatatata tcaatgaaaa ccattacaat aaccccaaat actgaaccat
    15721 atactaaatc atccataacc attgtaggct tatttattag atagccacac attataccaa
    15781 gtaatacact ctgacttgcc caaatagcag ttcctagatt tccaagtacc agagaaatta
    15841 ttaaaagcga agtaattcca gataaaatgg tatactttaa atcacatttc aagcagatta
    15901 tacaaaaaaa tactggcact ataaaatcta agtaaatagc atatccaaat ccagtactca
    15961 cagctataat tgttactact atgaataaag atgacaataa agctgcttca gttaattttt
    16021 tagtagtatt caaaacactg cctccataaa tcataaattt tctaactaaa gttttgtttt
    16081 taatatagta tatcactttt taaaaatatt tttaataaat ataaatttta ataaaggtat
    16141 ttatgttaaa aatggtataa tgtatcttaa tattcagttt tttaagatgc taggaggtaa
    16201 ttactatgtt taaaattaaa cactttaatg atttgagttt agatgaattt tatgaaatag
    16261 caaaatcaag atatgaggta tttgcttgtg aacagaagat attttcatta aacgattatg
    16321 atgatataga taaaagctct tatcacatat ttttaaaaga aaatggattg atttgtgctt
    16381 acgctagaat aattcctaaa gaatacagct cttataatga tgtttccatt ggtagagttt
    16441 tagttctttc tagtcataga agaaaaggat tagcaaaaca aatgatggat tgtgctatag
    16501 attttataaa agttaactta cacgaaaata atataacctt atcagcacaa acttatataa
    16561 agaaccttta cttatcttgt ggttttaaag aaatctcaga agtatacgac gaagcaggta
    16621 ttgaacatat taaaatgaga ttatagtgtt gacaagctaa tattgttaat atataattta
    16681 agtataaatt aaatattgaa ttgatattta ggtatttgaa aataccttac atagagtcag
    16741 aaggcttttg ctttctgact tttttattat caaaattcaa taattatatg gattcaaagt
    16801 gttaattttt attctatatt ctactttccc caaaagtaag aattataccc aactttgtat
    16861 gataggcatc ttgcctatct tttttttgta aaagtaacaa ggtaataata taatgcatag
    16921 tttaaaataa gaagattagc ccaggctaaa tttcaagcat ggaggtgcta ttatgaaaaa
    16981 ccttaacgat attcaagtga aaaaaactgt caaagttgaa gatatattat ctagtggtaa
    17041 tttaagagaa agaatgttag cgcttggtct tactagagga gctgttatag atgtagttag
    17101 aaaaggtcca aaaaataatt taactgtcta taaaatcaga ggtagcaaga tagctctacg
    17161 acaagaagaa agtagcttaa tattagtttc tgacatttaa aaatttctag caggaggata
    17221 ttaatatggg gttgacacat aactcaacta aaatgagttc tttaaaagac atgtttgata
    17281 tagacaataa ggaagaccag tttgtaattg cattggctgg caatccaaat acaggtaaaa
    17341 gtactgtatt taatcatctt acaggactta gacagcatac tggaaactgg ccaggaaaaa
    17401 ctgtagctac agctcgtggt aattttaaat ataaaaatac cgaatatgca ttaatagatt
    17461 taccaggaac atattcttta tttgccttat cacaagaaga aattgtagct cgtgatttta
    17521 tatgttttgg taatccagat gcagtaatag tagtatgtga tgctacatgt cttgaaagaa
    17581 atttaaattt agtatttcaa gtgatggaac ttacagataa agttattttg tgtattaatt
    17641 taatagatga agctagaaaa aaaggcatta ctattgataa aaaactttta gaggattctc
    17701 ttggtattcc agttatttta actgctgcaa gaaatggctc tggtatggat gaactattgg
    17761 atactttaaa tgatgtatct tttgacaaat ataaattaaa taataaacct gttagataca
    17821 atgaaaatat agagaatgtg gtaaaatcaa ttcaaccaga attggataat attataccag
    17881 gtataaattc tagatggttg ggacttagac ttattgatgg cgatgaaagt atatttgaat
    17941 ctatgagtaa ttatatagac aaagattcta tagatgctat taatgaagta aagaaaaaga
    18001 ttccagacaa tataaataaa cagaaaataa gagatgagtt tactaaaatc aactatgatt
    18061 atgctaaaaa actgagtgat gagtgttgtt ctaatgttgc taaaaaatca actgatagag
    18121 aagaaaaagt agataaaata ttaacatcta aaatatttgg gcttccaata atgctcttat
    18181 tattaggtac tatattgtgg attactatag aaggagctaa ctatccatcc actttattat
    18241 ctaatttatt actcggattt gagccaagta tttcgggtat attaaatagt attaattgtc
    18301 catcatggtt aaatgatatg ttggttttag gattatacag aacattagca tgggtaatct
    18361 ctgtaatgct acctcctatg gcaatatttt ttccattatt taccctactt gaggattttg
    18421 gatatttgcc aagagttgca tttaacttag accacctatt taaaaaagct tgtgcacatg
    18481 gaaaacagtg tttgactatg tgtatgggat ttggatgtaa tgcagctgga gttattggtt
    18541 gtaggataat tgactctcca agggaaagac ttatagctat acttacaaac aactttgttc
    18601 cttgtaatgg tcgttttcct actttgatag ctatatctac aatatttttt agctcagtta
    18661 taaccaactc tttcgtatca agcgtagcaa ctgcactttg cataacttta ctgataattc
    18721 taggtgttat aataacattg ttggtttctt atactctttc taaaacgcta ttaaaaggag
    18781 ttccgtctac atttacatta gaacttcctc cttatagagt tcctcaaata ggaagaactt
    18841 tatatacctc tataatagat agaacaatat ttgttttagg aagggctgta atggtagcca
    18901 tacctgctgg tgtcataact tggatatttg caaatatata tataggagat ttaagtatat
    18961 tatcacatgt tgcaaacttt ttagaccctt tagctaaatt aattggtctt gatggtttta
    19021 tattacttgc ttttattctt ggattcccag caaatgagat agttgtacct atacttttaa
    19081 tggcttacct agcaactggt tctatgatag aattagatag ctttagtgct ttaggtcaag
    19141 ttcttagaga acatggatgg acttatttga cagctttaaa tgttatgctg ttcagtttac
    19201 tgcattggcc ttgtgcaaca acacttttaa caataaaaaa agaaactggt agtttaaaat
    19261 ggactgcctt aggattctta atgcctacta tacttgcttt tgttgtttgt tttttaacaa
    19321 ctacagttta caatttattt atataattag tattaactga cagtagacat atattagaga
    19381 aaccatttaa aaatttatat actttattat aaagcataga gatatattca atgataagta
    19441 aaattactta acatttgaat atatctctat tttaattctt atctggatac ttggattgaa
    19501 atttcagtca tataattatc ttcattactt tcacaaaaaa agtcaagtat actctcctca
    19561 tacgaatttc caacaatttt atattcattt tcatatatat attttttcaa tttttcgtaa
    19621 gatttatatg aatcctcata ttttccaata tgttttatgc aagcatacat tccttctggc
    19681 tttacatata gctttttatc ctttattttt ttatctatct tcaaataaaa acttgatata
    19741 cttaaatagt ccccattttt tatattttca ttacttacta ttactcccaa ctcatatcct
    19801 tgatacaaat ttttcgattt acaataattg gtaaaatcta ttaattccat cataaaatca
    19861 ctattactca tattactcaa atcgcttgat agaactaaat attcttcaaa ctttttggat
    19921 atataaattt cttcactatt cattttagaa attccttcaa ctgttatatt tattctattt
    19981 tgcatcttag aacttatctt tcttagtttt tttatttttt ctcttatctt tttttcttct
    20041 tctttaagca ttttcacaca aagctgggga gttctatttt gtatatactc ttttatttcc
    20101 tctaaactca ttcctatttc tcttaatgat tgtaatatat caagtgtatc atattgataa
    20161 actgagtaat atctataatt atttttatct ttatattcag gagaaaaaat acctattttg
    20221 tcatagaaaa ttaatgtctg cttagatata ccacacaatt tagcaaattc acctgttgta
    20281 aaatactgtt tttccaaggg ttatacactc cttttctttt tgttgtctat atagttacta
    20341 tattctttat tatttactaa atcaagtagt tattatttta gaaaatcaat atggttttga
    20401 ttttaaagct taaatatcag ttagatgtca gttataaata ttttacccat tcccatcata
    20461 aaaaagatat cccctcttaa cttttggatg gaatattata tttatacttg ttagagtgaa
    20521 aattgtaata ggtgctacaa taagatattt aattaaacat aaagttaatt attgtaataa
    20581 aaaatcttta gataaattag agttaaagta tggaaatata gatagagaaa aagctgttaa
    20641 actagaaata ttttatcaat atttaataga acttgaatat atagaaatga gtttattaat
    20701 aagaagatta gatactgcaa taacatcatt agcattagta tctattataa caatagcatc
    20761 atattattta atcagaaaaa atgtgtaatt atgtaagcta tttataataa aacactgata
    20821 aaattaataa taaaaagata ataggatttc tatactacct ttttgttttt ataatatagt
    20881 gtaattttat tatatttttg tatgctaata taactagata atcataagat aacacattaa
    20941 aag
//
LOCUS       DAEPIN010000065        20395 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.065, whole genome shotgun sequence.
ACCESSION   DAEPIN010000065 DAEPIN010000000
VERSION     DAEPIN010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20395
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.065"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            <1..585
                     /locus_tag="KR250_002835"
     CDS             <1..585
                     /locus_tag="KR250_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBH0068751.1"
                     /translation="NNHIGLDLGIKEFCISSCGDFIENPKYLKKSLNKLAKLQRELSR
                     KTIGSLNRNKARLKVAGLQEHIANQRNDFLQKLSTKLIKENDIICIEDLQVKNMIRNR
                     KLSRLISDVSWSEFIRQLEYKANWYGRKIVKVGKFFASSQICNKCGYKNEEVKDLNIR
                     EWICPSCNETHDRDINASINILKEGLRLITIQNK"
     gene            703..1008
                     /locus_tag="KR250_002836"
                     /pseudo
     CDS             703..1008
                     /locus_tag="KR250_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438308.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
     gene            1021..1926
                     /gene="truB"
                     /locus_tag="KR250_002837"
     CDS             1021..1926
                     /gene="truB"
                     /locus_tag="KR250_002837"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HBH0068752.1"
                     /translation="MNKIISILKPTGMTSHDVVSRVRKILNIKKVGHTGTLDPDASGV
                     LPICIGKATKVCEVILNKDKSYICELTLGISTDTYDASGEILKKVDDFKFSNEDIERA
                     FDTQRGEINQLPPIYSALKVNGKRMCDLVRSGRQSEITLKTRRVNIKDIKILSIKGNK
                     VMFYVECSKGTYVRSICHDIGEYLGCGAHMSFLNRTSSGKFDLDNSITLEELELFYEN
                     KTLDKYLYDIDYVLDSFNYVVLNPNAIKYYSNGGSIDDKRFLKNNFDKDDEFVRVYST
                     DNFLGLGKLSKHNNTISVKSDKMFI"
     gene            2057..2986
                     /locus_tag="KR250_002838"
     CDS             2057..2986
                     /locus_tag="KR250_002838"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HBH0068753.1"
                     /translation="MVIIKSIKEIANIKESVITIGNFDGVHKGHQVLIGKTVEHAKKE
                     NIQSIVFTFANHPVNYFRPNSTKNIISNDDKIKLLEDLGIDIVVIIPFDEYMTKIPAK
                     DFVDEILVKKLKAKKIVIGHDFTFARAKEGNVNLLKSLEHEFGFEVEVIKPIKINDVR
                     VSSTYIRSLVSQGDMANVKEYLGRNYKLEGCVIHSKHLGRTIGFPTANIDLKNNMLVP
                     KRGIYASIVHIGDETYFGATNVGYNPTVNGKTLSIETNILEFDRDIYGENIIVEFLER
                     IRDERKFNSIDDLKNQLYNDTNYVYENYVCKNK"
     gene            3090..3347
                     /gene="rpsO"
                     /locus_tag="KR250_002839"
     CDS             3090..3347
                     /gene="rpsO"
                     /locus_tag="KR250_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002843739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HBH0068754.1"
                     /translation="MINKSEIIKEYGRAEGDTGSPEVQIALLTARINELNGHLKTHKK
                     DHHSRRGLLKMVGRRRNLLAYLKEKDLEGYRALIAKLGLRK"
     gene            3587..4432
                     /locus_tag="KR250_002840"
     CDS             3587..4432
                     /locus_tag="KR250_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBH0068755.1"
                     /translation="MKIKLVCDSLCDIPDEISEKDYIEIVPLTVIFNDREYIEGVDIK
                     KEEFYKKVKEIKQIPKTSQATYMEFKEVFDKFITEGYHIICMTGAANASGTFQSAMIA
                     KNDVNENEKIHIFDTRNLSLGSGQYVIKACELLEEGLGFEEIIDELENTRSSVRLLFA
                     PYTLDFLKQSGRVPVATALIGNMLNIKPIFFFDNGEAKLVNKVRGIKNIASKLVDIIL
                     EMNEGSLEGKIVTIGCGDNLHDCEILKDEVNKRIKARRVLFTRGGVSICSHTGPDILA
                     ISCSN"
     gene            4557..6668
                     /gene="pnp"
                     /locus_tag="KR250_002841"
     CDS             4557..6668
                     /gene="pnp"
                     /locus_tag="KR250_002841"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HBH0068756.1"
                     /translation="MFEHKIFKMDFAGRELSVEIGKICEMASGSCIVRYSDSMVMVNT
                     TKSAKPRDGIDFFPLSVDYEEKLYSVGKIPGGFLKREGKPSEKAILTSRLIDRPIRPL
                     FPKGFRNDVQVVATVLSVDQDCTPDIVAMIGSSIALSISDIPFNGPTGSVCVGLVDGA
                     FVVNPNAEQREKSSMHLVVSGTKEAIMMVEAGADEVPDEVMLDAILFAHQEIKKIVEF
                     IEGIVAEVGKEKMPVELYHAGEEITQLVREFATDKMKKAVQTFEKLERMENMDRVKEE
                     TLAHFEETLEDFEDFVGDIEEVLQDIIKEEVRKLIVHENVRPDNRKLEEIRPIWCETG
                     MIPRAHGSAIFTRGQTQVLNVATLGALGDVQKLDGLDEEENKRYMHHYNFPAYSVGEA
                     RPSRGPGRREIGHGALAERALLPVIPSQEEFPYAIRLVSEVLSSNGSTSQASVCGSTL
                     SLLDAGVPIKDMVAGIAMGLIKHDGKVAVLSDIQGMEDHLGDMDFKVAGTEYGITAIQ
                     MDIKIDGIDKEILQRALKQAKEGRIHILGEMRKTISQPKPELSPYAPKIVKMQINPDK
                     IKDVIGPGGKIITKIIDETGVKIDIEQTGEVFISGIEIDMIKKAQELINNIVVEPEVG
                     KTYKGKVSRIMNFGAFVEILPGKEGLLHISHIAHERVAKVEDVLNIGDEVEVKVTEID
                     EKGRVNLSRKVLLPKPEHKNK"
     gene            6934..7677
                     /locus_tag="KR250_002842"
     CDS             6934..7677
                     /locus_tag="KR250_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBH0068757.1"
                     /translation="MIMMVLNKSKLKKIIILILVVLLVLSGGVMVLKLFNNKPVFNLF
                     NGVDLPYERGTKDKSGYVALTCNIDLGWETEYVESILETLKKENVKITFNVTGKWAEK
                     NKDELLKIKKQGHEIGNHGYKHLDYSTLSYEDNYEQIETSKKIIEEIIGEKTKFFQAP
                     AGSFGPETVKAAKALGYTSIKWDADTIDWKYKDQPEVIIDRMKKKDIKDSSIILMHPT
                     NATTKCIDDIIAIVREKGLKPGKLSDVFK"
     gene            7835..9082
                     /locus_tag="KR250_002843"
     CDS             7835..9082
                     /locus_tag="KR250_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBH0068758.1"
                     /translation="MYKTKILENGLTIIGEEIPYLKSITLGIWINAGSRIEEAQVSGI
                     SHFIEHMMFKGTKNRTSKEIASSIDNLGGQINAFTSKECTCYYVKLIDEHIDTGIDVL
                     SDMILNSKFDKNDIDKERLIILEELKMYEDSPDDLSYDLLVENIYANDGLGMNIIGTK
                     ESLYNITRESMLEYLNKYYIPNNAVISIAGNFNFDDMVEKIKSKFGHWEKKNLSIDIS
                     EAKFNPCFISKNKDTEQVNLAMCLKGIPFENDEEVYSMAVVNNIFGGSISSRLFQKIR
                     EEKGLVYSIYSSQTLYRKCGELGIFASMSTENLQDVYNLIKKEIENIRENYLTEKEIS
                     ESKEQLKGNYILDLESTSSRMMSTGKSMLLSKKVKTTDEILECINNVNINSIKKVVDK
                     VFNIENIGTCIVGRDVEKILHLD"
     gene            9117..9362
                     /locus_tag="KR250_002844"
     CDS             9117..9362
                     /locus_tag="KR250_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HBH0068759.1"
                     /translation="MNLSEIAGKEIVNLVTGERLGVIGECDLILDETTGDILALLIPK
                     EKGFFARRKDKSFLEVPWRNVKKIGNDMIIIEYEGYI"
     gene            9372..10595
                     /gene="dapG"
                     /locus_tag="KR250_002845"
     CDS             9372..10595
                     /gene="dapG"
                     /locus_tag="KR250_002845"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HBH0068760.1"
                     /translation="MSILVQKFGGTSVESYEKMNEVCKIVKAYKKNDEELQLVLVVSA
                     MGRKGAPYATDTLINLCSAVNDEPSKRELDLIMSCGEIISGTILANLLNAQGIDSVFF
                     TGQQAGIITSDEYSNAKIKYINPKKVKRALDDGKVVVIAGFQGVTDDGEITTLGRGGS
                     DTSAVAIGKALECETVEIYTDVDGIMTADPRVEPNAKVLSFIDYEEVFQMADKGAKVI
                     HPRAVQLAKSGNITLAIKNTMNPTFEGTKIGSLCRHLEDNIEYEQERDFKVAVANKDS
                     VAQVKIKSAEEVFTEVLNEIEKKLITIDMINFFISEKAFVVEDADIKSLKEILDKFEL
                     DYEVNRDCAKVTLICSRIADEMSGIMSKVVRGLSKAGVSLLQTSDSNMTISCLVSEED
                     MHTAVHAIHQQFYLK"
     gene            10708..11442
                     /locus_tag="KR250_002846"
     CDS             10708..11442
                     /locus_tag="KR250_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HBH0068761.1"
                     /translation="MKDYREDNEKFDKDNCSEESSCEERECIKQFGTNEMPPQPPKDI
                     QCITIIGEIEGHFIGNPQKKATKYEHIIPMLYSIEESNDVKGVLVVLNTVGGDIEAGL
                     AIAELLNSTSKKVVTLVLGGSHSIGVPLATAGDYSFIAPTATMIIHPVRTTGLVIGIN
                     ETFEYFKKMQDRIIQFIIRTSNIKKDVLEKLMHEKDELVSDVGSVLIGKEAVDYGLID
                     EVGGLKEALKKLRELIKESEEEKNND"
     gene            11565..13976
                     /locus_tag="KR250_002847"
     CDS             11565..13976
                     /locus_tag="KR250_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HBH0068762.1"
                     /translation="MATKKKKKKKVNTLSFNAEYHNLITIFIGVFLLYSLNSNSMGWI
                     PVLMQNLFKGLFGGLSIAIPFIVIITGLLGFFDGNEYIYRLRKTKLYYIIILFIFVFY
                     GLLNAGTLPVDSPLKGNMFDDVMKLGVSGQGSGLIATTIAYYMSKIFGIAGGWLISIF
                     ALILSVMFIFNISIKDLVSNAKSKSKASKDSNLTFKDKIANMKKSAIDMMTDEVDDTT
                     INKKPGFFKGLMSKGLNNDDEEDEYFEEENSDGVDDKTIKIVGFNKAEDEYLEILEGT
                     QSMPELDVLKELQKATNENPVVDTKPEKKVDIAKPNLNIEKTQPMSIVAEPVNEDYSN
                     YKKPSIELLNKVNKKSDENGKKKVLKNASLLEKTLSDFGVEAKINQVTVGPTITRYEI
                     QPSPGVKVSKIVNLTDDIALSLAAKSIRIEAPIPGKSAIGIEVPNEEAQMVGVREVLE
                     SEEFNNFNSPLAMGLGKDVAGKIIIGDIGKMPHLLIAGSTGSGKSVCVNTLISSILYK
                     ANPDEVKLLLIDPKVVELANYNGIPHLLIPVVTDPKKAANALNWAVTEMNRRYKLFAD
                     AQVKDVTSYNEKAEEKLPKIVIIIDELADLMMASANDVEDYICRLAQMARAAGMHLIV
                     ATQRPSVDVITGVIKANIPSRIAFAVSSQTDSRTILDMGGAEKLLGKGDMLFYPLGAA
                     KPVRLQGAFISESESEKVIDFVKSQVKDGIKYEEDIIETISKVNTSKGSDEDEFLSEA
                     IEFVVESGQASASMLQRRFKIGFNRAARLIDSMEERGIIGASEGSKPRKVLISKQDLQ
                     NLEGE"
     gene            13979..15037
                     /gene="mnmH"
                     /locus_tag="KR250_002848"
     CDS             13979..15037
                     /gene="mnmH"
                     /locus_tag="KR250_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HBH0068763.1"
                     /translation="MSVIKIEKALKLDKVIFVDVRTEGEYEEDHILNAINMPLFKNNE
                     HNEVGTIYKMQGKHEAIQKGFDYVSYKLKDIYLQAAELALNYDNIVIYCARGGMRSGS
                     IVNLLSSLGVNVYQLEGGYKAYRNFVLEYLRHVMDTKNFVSVHGLTGTGKTDLLHLLD
                     EKGIDVLDLEGIAKNSGSTFGFITFDKKPPSQKNFETKLFESIFFSKENYIFVESESK
                     RVGSVTVPNEIFEAMTREGHHILLECDIENRVDRLCRDYIYDKDEGNILILKECINKF
                     RKRLGNKVVDDYIDLLESGEYKELVKRYLLEYYDPLYMHSVEKYKYSKIINFNDTQKA
                     LEDVIDLYESILEGESEC"
     gene            15031..16365
                     /gene="rimO"
                     /locus_tag="KR250_002849"
     CDS             15031..16365
                     /gene="rimO"
                     /locus_tag="KR250_002849"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HBH0068764.1"
                     /translation="MLKIALESLGCSKNLVDAEIMMGILNNKGYKLIGDFEEADVIIV
                     NTCGFIESAKQESIDTIINFAELKKTGNLKLLIVTGCLAQRYSEELKTEIPEIDAIVG
                     TGSYQNIDKILKELSEMHQIVSLNDIEFVFNEDLPRYISTPSYMAYLKIGEGCSNNCT
                     YCIIPKLRGKYRSRKFEDIIKEAKKLAESGVKELVVIAQDTTKYGFDLYGKERLSELL
                     EELAKIDGFKWIRVMYSYPESITEELIQVIKKYDNICSYFDMPIQHASNNILKLMNRK
                     TTKEDILNKINLIRSNIPDAILRTTIIVGFPGETEDDFKQLVDFVEEVKFDRLGAFAY
                     SREEDTPADRLPNHIDEEVKIQRRDTLMMIQQKISEELNDKKIGKTYEVLIEEQIEDN
                     VYTGRTQGDAEEIDSIVYVKSVDNLEVGEFVSVQINDAMEYDLMGDVLYELA"
     gene            16352..16894
                     /gene="pgsA"
                     /locus_tag="KR250_002850"
     CDS             16352..16894
                     /gene="pgsA"
                     /locus_tag="KR250_002850"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HBH0068765.1"
                     /translation="MNLPNKLTLFRIFLIPVFVLIMLLNVPNKFLIACIIFIIASITD
                     ALDGKIARKYNLVTDFGKFMDPLADKLLVISALTCMIEDHLVSSWMVIIIVARELTVS
                     ILRAIAAADGKVIAAGNSGKLKTITQMVSIVFLLLGAQFENVLILNIGEILILIATLL
                     TLYSGWEYLYKNKELFMSSK"
     gene            17182..18228
                     /gene="recA"
                     /locus_tag="KR250_002851"
     CDS             17182..18228
                     /gene="recA"
                     /locus_tag="KR250_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HBH0068766.1"
                     /translation="MSVDQEKLKALNEALGKIEKDFGKGSVMKLGEATSMSIDVISTG
                     AIGLDIAIGIGGLPRGRIVEVYGPESSGKTTVALSCVASAQKDGGIAAFIDAEHALDP
                     VYAKALGVDVDNLIISQPDTGEQALEIAEALIRSGAIDIIVIDSVAALVPKAEIDGDM
                     GDSHVGLQARLMSQALRKLTGSIKKSNCVAIFINQLREKVGIMFGNPETTTGGRALKF
                     YSSVRLDVRKIDTIKQGDKVIGSRTRVKVVKNKVAPPFKQAEFDIMYGEGISKIGDLL
                     DIAADVDIVKKSGSWYSYNDTKLGQGRENVKKFLEDNLDLTTEIDEKVRAFYNLNEEH
                     EESGISVSKEIVEE"
     gene            18404..19945
                     /gene="rny"
                     /locus_tag="KR250_002852"
     CDS             18404..19945
                     /gene="rny"
                     /locus_tag="KR250_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HBH0068767.1"
                     /translation="MDVIDSVVMIVIGAAVGIIAGYFVRKNISEAKIGQAENLAKEII
                     DKAHHDSETVQKEKLLEAKEEIHKWRTEAERENRERRTEVQKYEKRVVQKEEVLDRKL
                     QNLESKEVNLSEKLKVVEKKEEEVEVIKTQQLEKLESISGITSDKAKEIILTNAERDV
                     RREMSIMIKEIESQAKEEADKKSREIIGYAIQKCAADHVAETTVTVVSLPNDEMKGRI
                     IGREGRNIRTLETLTGIDLIIDDTPEAVILSGFDPIRREIARIALEKLIADGRIHPAR
                     IEEMVEKARKEVDNIIKEYGEQAAFETSVHGIHPELIRLLGRLNYRTSYGQNVLKHSI
                     EVAHIAGIMAAEIGADIRLAKRAGLLHDIGKAVDHEMEGTHVEIGMDLLRRYKESKEV
                     IHAMSTHHGDYEPQTVEAVLVTAADAISAARPGARRETLEAYIKRLEKLEEIANSYEG
                     VEKSFAIQAGREIRIMVKPEAINDEEIHLLARDMTKKIEDELEYPGQIKVSIIRETRA
                     IEYAK"
BASE COUNT         8352 a         2059 c         3921 g         6063 t
ORIGIN      
        1 aataatcata taggtttaga tttaggaatt aaagaatttt gtatctcaag ttgtggagac
       61 tttatagaaa atcctaagta tcttaagaag tcattaaaca agcttgctaa attacaaaga
      121 gaattatcga gaaaaacaat cggtagttta aatagaaata aagcaagatt aaaagttgca
      181 ggacttcaag aacatatagc taatcaaaga aatgattttc tacaaaaact atctactaaa
      241 ttaataaaag agaatgatat tatttgcata gaagatttac aagtaaagaa tatgattaga
      301 aatcgtaaac tatctcgctt aatttcagat gtatcatggt cagaatttat tcgtcaatta
      361 gaatacaagg ctaattggta tggtagaaaa attgtaaagg taggtaaatt ctttgcaagt
      421 tcacaaatat gcaataaatg tgggtataaa aacgaggaag ttaaggattt aaatataaga
      481 gaatggattt gtcctagttg taatgaaact cacgatagag atataaatgc aagtataaat
      541 atattaaaag aaggattaag actaataacg attcaaaata aataacttat gtaagaaccg
      601 taggaactac ggggatagcc tgtggagaat tagtaagaca tattttaata tgcaaaattc
      661 tgtgaagcag gaaccctgtg actttagtca tgggaggttc agtcaaaggt atctagtata
      721 gttttaactc aagacatgat gaatagaaat aatattagct ttaatgatgt tgatggaata
      781 acaacatata caagagacat aaaaggtata gaagttggaa ttttatttaa agagaaaaaa
      841 caaaatgaaa ttaaagtgag ttttaggtca aaaaattatg ttgatgtaag tgaaatagcc
      901 aagttatttg gtggtggtgg tcatgtaagg gctgctggat gtactattag agatagtatt
      961 gataatgcaa agaaaatggt actagaggca gtgttaaaat caatttaagg tgacagtaaa
     1021 atgaataaga ttataagtat attgaaaccc acaggtatga catctcatga tgttgtaagt
     1081 agagtgagaa agattttaaa tataaaaaaa gttggtcata caggtactct tgacccagat
     1141 gcttcaggtg ttttaccaat ctgtataggg aaagctacaa aagtatgtga agttatttta
     1201 aacaaagata aatcgtatat atgtgagctt actttaggta taagtacaga tacttatgat
     1261 gcttcaggtg aaattttaaa aaaagttgat gattttaaat ttagtaatga agacatagaa
     1321 agagcttttg acacacaaag aggagagata aatcaacttc ctccaattta ttcagcttta
     1381 aaggtaaatg gaaagagaat gtgtgattta gtaagaagtg gtagacaatc tgaaattact
     1441 ttaaaaacaa gaagagtaaa tataaaagac ataaaaatac taagtataaa aggtaataaa
     1501 gtaatgtttt atgttgagtg ttctaaaggg acttatgtga gaagcatttg ccatgatata
     1561 ggagaatatc taggttgtgg agctcatatg tcgtttttga atagaacatc ttctggtaaa
     1621 tttgacttag ataattcaat aacattagaa gaattagaat tattttatga aaataaaact
     1681 ttagacaaat acttatatga tatagattat gtccttgatt cgtttaatta tgttgtatta
     1741 aaccctaatg ctataaaata ttatagtaat ggtggctcaa tagatgataa aaggttttta
     1801 aaaaataatt tcgataaaga tgatgaattt gtaagagtat acagtacaga taatttctta
     1861 ggattaggaa aattatcaaa acacaataat acaataagtg ttaaaagtga taagatgttc
     1921 atataaccaa tgtatttaaa aaaacaacaa aagagatagt actataataa cttctatagt
     1981 ctatctcttt tacttatgct ataatagttt gtgaataagt taagagtaaa ttatatttta
     2041 gcggggaaca tttattatgg tgattataaa atctataaaa gaaatagcaa acataaaaga
     2101 aagtgtcatt accataggaa attttgatgg tgtacacaaa ggacatcaag ttcttatagg
     2161 aaaaacagtt gaacatgcaa aaaaagaaaa tatacagagt atcgtattta catttgcaaa
     2221 tcatcctgta aattacttta gaccaaactc aacaaaaaat attataagca atgatgataa
     2281 aataaaatta ttagaagatt tgggaataga tatagttgta atcataccat ttgatgagta
     2341 tatgactaaa atacctgcaa aggatttcgt agatgagata ctagtaaaaa aattaaaagc
     2401 taaaaagata gtaattggac atgactttac atttgcaagg gcaaaagaag gcaatgttaa
     2461 tctattaaaa agcttagaac atgaatttgg ttttgaggta gaagtaataa aacccatcaa
     2521 aatcaatgat gtaagagtga gtagtactta tattagaagt ttagtatccc agggtgatat
     2581 ggctaatgtc aaggaatatt tgggtagaaa ttacaagtta gaaggttgtg ttatacattc
     2641 taaacacctt ggaagaacta taggattccc aacggcaaat attgacctta agaataatat
     2701 gctcgttcca aagagaggta tttatgcttc tattgtacat attggagatg agacttattt
     2761 tggtgctaca aatgtaggct ataacccaac agttaatgga aaaactttgt ctattgaaac
     2821 aaatatttta gagtttgata gagatattta tggggaaaat ataattgttg aatttttgga
     2881 aagaataaga gatgaaagaa agtttaattc aatagatgac ttaaaaaatc aattgtataa
     2941 tgatacaaat tatgtgtatg aaaactatgt ttgcaaaaac aagtaatgta tgataaaatg
     3001 attaaggaaa ataaccatta ctcagcattt gaatactcca atcaatgctt agtaataggc
     3061 gtttctaaaa aaatacggag gtaatctaaa tgataaacaa atcagaaata ataaaagaat
     3121 atggaagagc tgaaggggat actggttctc cagaagtaca aatagcttta ttaacagcta
     3181 gaataaacga gttaaatgga catttaaaaa ctcataagaa agaccaccat tcaagaagag
     3241 gactattaaa aatggttggt agaagaagaa acttattagc ttacttaaag gaaaaagatc
     3301 ttgaaggata tagagcatta atagctaaat taggattaag aaagtaattt cattctttat
     3361 aaaaaagcag gtatagtgta tacctgcttt tttatagtta attcattata aagtaaaata
     3421 gttaataata aaagtagttt atatgttata aaaaattata aaatgaataa tattgggtaa
     3481 gaatattaaa atagtataca aatacataat caatttaaaa ggaaagacag gataaatata
     3541 gaaattttat aacaggataa ttagttggaa taaggaggat attattttga aaataaagtt
     3601 agtatgcgat agtttgtgcg atattccaga tgaaatatca gaaaaagatt atatagagat
     3661 agttccactt acagttattt ttaatgatag ggaatatata gaaggagtag atataaagaa
     3721 agaagagttt tataaaaaag taaaagaaat caagcaaata cctaaaactt cacaagctac
     3781 atatatggag tttaaagaag tgtttgataa gttcataaca gaaggttatc atataatctg
     3841 tatgacaggg gctgcaaacg catcaggaac ttttcaaagt gctatgatag ctaaaaatga
     3901 tgtaaatgaa aatgaaaaaa tacatatatt tgatactaga aatttatctc taggtagtgg
     3961 acaatatgtt ataaaggcat gtgaacttct tgaagaagga ctgggatttg aagaaattat
     4021 agatgaatta gaaaatactc gcagtagtgt aagattgctt tttgcaccat atacacttga
     4081 ttttttaaag cagagtggaa gagttccagt tgcaactgct ttgataggaa atatgttaaa
     4141 tataaagcct atatttttct ttgataatgg ggaagctaag ttggtaaata aggttagagg
     4201 aataaagaat atagcaagta aattggtcga tataatttta gaaatgaatg aagggtcttt
     4261 agaaggaaaa atcgtcacaa taggttgtgg agataattta cacgattgtg aaatattaaa
     4321 agatgaagta aataaaagaa taaaagcaag aagagtatta tttactcgag gaggagtttc
     4381 tatttgctct catacagggc cagatatatt ggctataagt tgttctaatt agtagtaata
     4441 taaggttttt gcatatatat gtgaagataa ttttatattg ttaatttata gtatttttga
     4501 taatatagta aatagagtga tatacgtgtt ttttaataag aggaggacaa gcattaatgt
     4561 ttgaacataa aatatttaaa atggattttg caggaagaga actttctgta gaaataggaa
     4621 agatttgtga aatggctagt ggtagttgta tagttcgata cagtgatagt atggtaatgg
     4681 taaatacaac taaatcagcc aaacctagag acggaataga cttttttcct ttaagtgtgg
     4741 attatgaaga aaaattatac tctgtaggta agatacctgg aggtttttta aagagagaag
     4801 gcaagccttc agagaaagct atacttacat caagattgat agatagacca ataagacctt
     4861 tattcccaaa aggatttaga aatgatgttc aggtagttgc aacagttcta tcagtagacc
     4921 aagattgtac accagatata gtagcaatga taggttcttc aatagccctt tctatatcag
     4981 acataccatt taatggacca acaggttcag tatgtgttgg ccttgtagat ggtgcatttg
     5041 ttgtaaatcc aaacgcagag caaagagaaa aaagttcaat gcatctagtt gtttcaggta
     5101 caaaggaagc aataatgatg gttgaagccg gagctgatga agttccagac gaggtaatgc
     5161 ttgatgctat tttatttgct caccaagaaa taaaaaaaat agttgagttt atagaaggta
     5221 tagttgcaga agttggaaaa gaaaaaatgc ctgttgaatt ataccatgca ggagaagaaa
     5281 taactcaatt ggttcgcgaa tttgcaactg ataagatgaa aaaagcagtt cagacttttg
     5341 aaaagttaga gagaatggaa aatatggata gagttaaaga agaaacttta gctcactttg
     5401 aagaaacttt agaagatttt gaagattttg ttggagatat agaagaagta ttacaagata
     5461 taataaaaga agaagtaaga aaacttatag tacatgaaaa tgtaagacca gataatagaa
     5521 aacttgaaga aataagacct atatggtgtg aaactggcat gatacctaga gctcatggtt
     5581 cagccatatt tactagagga caaactcaag tacttaatgt tgctacttta ggtgctttag
     5641 gagatgttca aaaactagat gggttagatg aagaagaaaa caagagatat atgcatcatt
     5701 ataatttccc tgcatatagt gttggagaag ctagaccatc aagaggacca ggaagaagag
     5761 aaataggaca tggtgcttta gcagaaagag ctttattacc agtaatacca tctcaagaag
     5821 aatttcctta tgctattaga ttagtttctg aagtattaag ttcaaatggt tctacatctc
     5881 aagcaagtgt atgtggctca acattatctt tacttgatgc aggtgtacct ataaaagata
     5941 tggttgcagg tatagcaatg ggacttataa agcatgatgg taaggttgca gtgctttcag
     6001 atatacaagg tatggaagac catttaggcg atatggactt taaagtagct ggaacagagt
     6061 atggtataac agcaatacaa atggatataa aaattgatgg tatagataaa gaaattctac
     6121 aacgtgcttt aaaacaagct aaagagggta gaatccatat actaggagag atgagaaaaa
     6181 ctatatctca gccaaaacca gaactttcac catatgcacc taagattgtt aaaatgcaaa
     6241 taaatcctga caagataaaa gatgtaatag gacctggtgg aaagataata actaagataa
     6301 tagacgaaac tggagttaag atagatattg aacaaactgg ggaagtattt atatcaggta
     6361 ttgagattga tatgattaag aaagctcaag aacttataaa taatatagta gttgaaccag
     6421 aagtaggaaa aacttataaa ggtaaggtaa gtagaataat gaactttgga gcatttgttg
     6481 agatacttcc aggaaaagaa ggattacttc atatatctca tatagctcat gaaagagttg
     6541 ccaaagttga agatgtattg aatatagggg atgaagttga agttaaagtt acagagattg
     6601 atgaaaaagg aagagttaat ttatcaagaa aagttctttt accaaaacca gaacataaaa
     6661 ataaataact tattatataa ttaaaagcaa agtcttagga ttttgctttt ttaaatacaa
     6721 aaaagtagat aattttaaaa tgatagttat ggatggctta tagttctttt tctgtcaggc
     6781 tttactctta taaagaagct atttaaaagc tgattaatag agagctttat tgatatatat
     6841 agaatcactt aagttatatt tagtgcataa acagagctta tttttaatag tattaaataa
     6901 gaaacaattt gtacataatt agggggagtt ttaatgataa tgatggtact taataagagt
     6961 aagctcaaaa aaatcataat actaatttta gtagtgttat tagtattatc aggaggggta
     7021 atggtattaa agttatttaa caacaagcca gtatttaatt tatttaatgg tgtagattta
     7081 ccatatgaaa gaggtacaaa ggataaaagt ggatatgttg cattaacctg taatatagac
     7141 cttggatggg aaacagagta cgtagaatcc attttagaaa cacttaaaaa agaaaatgta
     7201 aagattacat ttaatgtaac tggaaaatgg gcagaaaaga ataaggatga attattaaag
     7261 ataaaaaaac aaggacatga aataggtaat catggataca aacatttaga ttattctaca
     7321 ttatcatatg aagacaatta tgagcaaata gagacctcta aaaagataat tgaagaaatt
     7381 ataggtgaaa aaactaaatt ttttcaagca ccagcaggtt cttttggtcc agaaacagta
     7441 aaggctgcta aagcattagg gtatacttct atcaaatggg atgccgatac tatagattgg
     7501 aaatacaaag accaaccaga ggtaatcatt gatagaatga agaaaaaaga cattaaagat
     7561 agtagtataa ttttaatgca tccaaccaac gcaacgacaa aatgtataga tgatattata
     7621 gctattgtaa gagaaaaagg actaaaacca ggaaaactta gtgatgtatt taagtagaca
     7681 aagtataaat atttggttaa gattgtataa gttgtagttg aattaattaa aagactgact
     7741 tataatgtaa tacatataaa aaataaatat aattaaaaca tagtgttttc aataatccaa
     7801 tatgaaacaa agaataaatt gttaggagaa tgtaatgtat aagacaaaaa tactggaaaa
     7861 tgggcttaca ataattggtg aagaaatacc atatctaaag tcaataacat taggtatttg
     7921 gataaatgca ggctctagga tagaagaagc acaggtgagt ggaatttctc attttataga
     7981 gcatatgatg tttaaaggaa ccaaaaacag aacatcaaaa gagattgcta gttcaataga
     8041 taatttgggt ggtcaaataa atgcatttac aagtaaagaa tgtacatgtt attatgtaaa
     8101 actaatagat gagcatatag atacaggaat tgatgtattg agtgatatga tactaaattc
     8161 taaatttgat aaaaatgata tagataagga aagacttata atattggaag aacttaagat
     8221 gtatgaagac tctccagatg acttatctta tgatttatta gtggaaaata tatatgctaa
     8281 tgatggatta ggaatgaata taattgggac aaaagaatct ctgtacaata taactagaga
     8341 atccatgtta gaatacttaa ataagtatta tattccaaat aacgctgtta tttctatagc
     8401 tggaaacttt aactttgatg atatggtgga gaaaatcaaa tcaaaatttg gacactggga
     8461 aaagaaaaat ttaagtatag acataagtga ggctaagttt aatccttgct ttatatcaaa
     8521 aaataaagat actgaacagg tgaacttagc tatgtgccta aaaggtattc cttttgaaaa
     8581 tgatgaagaa gtctattcaa tggcagtggt aaataatatt ttcggtggaa gtataagctc
     8641 aaggcttttt cagaaaataa gggaagaaaa aggtttagta tattcaatat actcatcaca
     8701 gacattatat aggaaatgtg gtgaattagg tatatttgca agcatgagta cagaaaattt
     8761 acaagatgta tataatttaa taaagaaaga aattgaaaat ataagagaaa actatcttac
     8821 agaaaaagaa atatctgaaa gcaaggaaca gttaaaagga aattatatat tggatttaga
     8881 aagtactagc agtagaatga tgtctactgg taaatctatg ttacttagca aaaaagtaaa
     8941 aactacagat gaaatacttg aatgtataaa taatgttaat attaattcaa taaagaaggt
     9001 tgttgataag gtattcaata tcgaaaatat tggtacatgt atagttggaa gagatgtaga
     9061 aaagattcta cacttggatt aatattgaaa aaagtaaatc tctggaggta taaaatatga
     9121 atttatctga aatagcaggt aaggaaatag ttaacttggt cacaggagaa agattggggg
     9181 tcattggaga atgtgacctt atactagatg aaactacagg cgatatatta gcgcttctaa
     9241 ttccaaaaga aaaaggattt tttgccagaa gaaaagataa gtcattttta gaagtaccat
     9301 ggagaaatgt aaaaaaaata ggtaatgaca tgataattat agaatatgag ggatatattt
     9361 aggaggaaaa aatgagtatt ttagtgcaaa agtttggtgg gacatcagta gaatcatatg
     9421 aaaaaatgaa tgaagtatgt aagatagtaa aagcatacaa aaaaaatgat gaagaattac
     9481 aattagtatt agtagtttca gcaatgggaa gaaaaggagc tccatatgct acagatacac
     9541 ttataaattt gtgtagtgca gtaaatgatg agccaagtaa aagagaactt gatttaataa
     9601 tgtcttgtgg agaaataata tcaggaacaa ttcttgctaa tttattgaat gcacagggga
     9661 tagattctgt tttctttact ggtcagcaag caggtataat tacaagtgat gaatattcta
     9721 atgcaaagat aaagtatata aatcctaaaa aggtaaaaag agctttagat gatggtaagg
     9781 ttgtagttat agctgggttc cagggtgtaa cagatgatgg agaaataact actcttggaa
     9841 gaggcggaag cgatacatca gcagttgcta ttggtaaagc tttagaatgt gaaacagttg
     9901 aaatttacac tgatgtggat ggtataatga ctgcagaccc tagagtagaa ccaaacgcta
     9961 aagtattgag ttttatagac tatgaggaag tattccaaat ggctgacaaa ggtgcaaaag
    10021 taatacatcc aagagcagta cagttggcta aaagtggaaa tataacttta gccataaaga
    10081 atacaatgaa tccaactttt gaaggtacaa aaataggttc tttatgtaga cacttagaag
    10141 acaatataga atatgaacaa gaaagagatt ttaaggttgc tgttgcaaat aaagatagcg
    10201 tagcacaagt taagattaag tcagcagaag aagtgtttac agaagtatta aatgagatag
    10261 aaaagaaact tataactata gatatgataa atttctttat atcagaaaaa gcatttgttg
    10321 tagaagatgc tgatataaaa tcattaaagg aaatactaga taaatttgaa ttagattatg
    10381 aagtaaatag agactgtgct aaggtaacat tgatatgttc aagaattgct gatgaaatgt
    10441 ctggtatcat gtcaaaggta gttagaggat tatcaaaagc aggggtatct cttttacaaa
    10501 catcagactc aaatatgact atatcttgtt tagttagtga agaagatatg catacagcag
    10561 tacatgctat acaccaacaa ttttatttaa aataaactgt ataatacaat tattttttaa
    10621 tcaaatttaa gaatttcata tatatcttaa tatatttgat ttatttggta aaactaaatt
    10681 aaatataatt ttgataggag tttttttatg aaagattata gagaagataa tgaaaaattt
    10741 gataaagata attgtagtga agaatctagt tgcgaagaga gagaatgtat aaagcaattt
    10801 ggtaccaatg aaatgccacc tcaacctcct aaagatattc aatgtataac tataattgga
    10861 gaaattgagg gacattttat aggaaatcct caaaaaaaag ctacaaagta cgagcatata
    10921 attccaatgc tatactcaat tgaggaaagt aatgatgtta aaggagtgtt agttgtttta
    10981 aacactgttg gaggagatat tgaagcaggt ttagctattg cagaactttt aaacagtaca
    11041 tctaaaaaag tagttactct tgttctagga ggaagtcata gtataggtgt tcctcttgct
    11101 acagcaggag attactcatt tatagcacca acagcaacca tgattataca tccagtgcga
    11161 actactggtc ttgtgattgg tataaatgag acttttgaat attttaaaaa gatgcaagat
    11221 agaataattc aatttataat aagaacatca aatatcaaaa aagatgtact tgaaaaactt
    11281 atgcatgaaa aagatgaact tgtaagtgat gttggtagtg tattaatagg aaaagaagct
    11341 gtggattatg gtcttataga tgaagttgga ggactaaaag aagcacttaa aaaattaaga
    11401 gagcttataa aagaatctga ggaagaaaaa aataatgatt aaaatttgtt taaataataa
    11461 aatttatgat ataatgatgt aataaagtag gagtgttagt gttaaggcat tagcactttt
    11521 tgctgtgaag gggtaaaact tgctaaaaat aaggagtgat attaatggcc acgaaaaaga
    11581 agaaaaaaaa gaaggtaaat accttaagtt ttaatgcaga atatcataat ttaataacaa
    11641 tatttatagg tgtattttta ttatatagtt taaattctaa ttctatggga tggatacctg
    11701 tgttaatgca aaatcttttt aagggactat ttggagggct ttcaatagca ataccattta
    11761 tagtaattat aacaggactt ttaggatttt ttgatggaaa tgaatatata tataggctaa
    11821 gaaagactaa actatactac ataattattt tatttatatt tgtattttat ggattattaa
    11881 atgcaggaac tcttcctgta gatagcccac taaaaggtaa catgtttgat gatgttatga
    11941 aattgggtgt aagtggacaa ggtagtggtt tgatagcaac aactattgcg tattatatga
    12001 gtaaaatatt tggtatagca ggtggatggt taataagtat cttcgcactt atactttctg
    12061 taatgtttat atttaatatt tctataaaag atttggtgtc aaatgcaaaa tcaaaatcta
    12121 aagcatctaa agacagcaat ttaacgttta aagataaaat tgcaaatatg aaaaaatctg
    12181 caatagatat gatgactgat gaagtagatg atactactat aaataaaaaa ccaggtttct
    12241 ttaaagggtt aatgtcaaaa ggtcttaata atgatgatga agaagatgaa tattttgaag
    12301 aagaaaatag tgatggtgta gatgataaaa ctataaaaat agttggattt aataaggcag
    12361 aagatgagta tttagaaata ttagaaggaa ctcaaagtat gccagagtta gatgttttaa
    12421 aagaattaca aaaagctacc aatgaaaatc cagttgtaga tactaaacca gaaaaaaaag
    12481 ttgatatagc taaacctaat ttaaatatag aaaaaactca acctatgagt attgtagcag
    12541 agccagttaa tgaggattat tcaaactata aaaagcctag cattgagctt ttaaataaag
    12601 taaataagaa gtctgatgaa aatggtaaaa agaaagtgtt gaaaaatgct tctcttttag
    12661 aaaagacact atcagatttt ggagtggaag caaaaattaa tcaagttaca gttggaccta
    12721 ctataacaag atatgaaata caaccaagtc caggagtcaa ggtaagtaaa attgtaaatt
    12781 taactgatga tatagcttta agtttagcag ctaaaagtat aagaattgaa gccccaattc
    12841 ctggtaaaag tgcaattgga atagaagttc caaatgaaga agctcaaatg gttggtgtga
    12901 gagaagttct agaaagtgaa gaatttaata attttaactc tcctcttgca atgggacttg
    12961 gaaaagacgt agcaggaaaa attataattg gagatatagg taagatgcca cacttactaa
    13021 tagctggttc tacaggttca ggaaagagtg tgtgtgtaaa tacattaata agtagtattt
    13081 tatataaggc aaatcctgat gaagtaaaat tactattaat agacccaaaa gtagttgaac
    13141 ttgcaaatta taatggaata ccacatctat tgataccagt agttactgac cctaaaaaag
    13201 ctgcaaatgc tttaaattgg gcagttacag agatgaatag aaggtacaaa ttatttgcag
    13261 atgctcaagt aaaagatgta acaagttata atgagaaagc ggaagaaaaa cttcctaaaa
    13321 tagtaataat aatagatgag ttagcagatt taatgatggc aagtgcaaat gatgtagaag
    13381 attatatttg tagattagct caaatggcaa gagctgctgg aatgcacttg attgtagcaa
    13441 ctcaaagacc atctgttgac gttataacag gggtaataaa ggctaatata ccatctagaa
    13501 tagcatttgc agtatcatct caaactgatt caagaacaat ccttgatatg ggtggtgcag
    13561 agaagcttct aggtaaaggt gatatgttat tctatccact tggtgcagca aaaccagtta
    13621 gacttcaagg ggcattcata tcagagagtg agtcggaaaa agtaatcgat tttgtaaaaa
    13681 gtcaggttaa agatggaata aaatatgaag aagatataat tgaaactata tctaaggtta
    13741 atacttcaaa gggtagtgat gaggatgagt ttttgagtga agcgatagaa tttgtcgtgg
    13801 aaagtggtca agcatctgcc tctatgctac agagaagatt taaaatagga ttcaatagag
    13861 cagctagact tatagattct atggaagaaa gaggaataat tggagcaagt gagggaagta
    13921 aaccgagaaa ggtattaata agcaaacagg atttgcagaa tttagaaggt gaatagaatt
    13981 gtcagttata aaaatagaaa aagcacttaa gctagataaa gtaatatttg tagatgtgag
    14041 aactgaagga gaatatgaag aagaccatat attgaatgca atcaatatgc ctctttttaa
    14101 aaataatgaa cataatgaag ttggaacaat ttataagatg cagggaaaac atgaagcaat
    14161 acaaaaagga tttgactatg tatcgtataa attaaaggat atatatttac aagcagcaga
    14221 acttgcatta aattatgata atattgtcat atattgtgca agaggcggaa tgagaagtgg
    14281 cagtatagta aatttattat catcattagg tgtaaatgta tatcaacttg aaggtggata
    14341 taaggcttat agaaattttg ttttagagta tttaaggcat gtaatggata caaaaaactt
    14401 tgtatctgtt catggattga ctggtacagg aaagactgat ttgttacatt tattagatga
    14461 aaaaggaata gacgtgctgg atttagaagg catagctaaa aacagtggtt ctacctttgg
    14521 ttttataact tttgataaaa agccaccatc tcaaaagaac tttgaaacaa aactatttga
    14581 aagtatattt ttttcgaaag agaattatat atttgtcgag agtgaaagca aacgagttgg
    14641 aagtgttaca gttccaaatg aaattttcga ggcaatgaca cgagaaggac atcatatatt
    14701 attagaatgt gatattgaaa atagagttga tagactttgt agagattata tttatgataa
    14761 agatgaaggt aatatattga ttttaaaaga atgtataaac aagtttagaa aaagattggg
    14821 taataaagta gtagatgatt atattgattt attagaatca ggtgaatata aagaacttgt
    14881 aaaaagatat cttttagaat actacgaccc actttatatg cattcagtag agaaatataa
    14941 gtatagtaag attataaatt ttaatgatac acaaaaagct ttagaagatg ttatagactt
    15001 atatgagagt atattggaag gagaaagtga atgttaaaaa tagcattgga atcattagga
    15061 tgctcaaaaa atttagtaga tgcagaaata atgatgggaa ttttaaataa caaaggctat
    15121 aaattaattg gagattttga agaagctgat gtaattatag taaatacatg tgggtttata
    15181 gagtcagcta aacaagaatc tatagatacg ataataaact ttgcagagtt aaaaaaaact
    15241 ggcaatttga agttgttgat agtaacaggg tgtctagccc aaagatattc tgaagaatta
    15301 aaaactgaaa ttcctgaaat agatgctata gtaggaacag gaagctatca aaatatagat
    15361 aaaatactta aagaattaag tgaaatgcat caaattgtaa gccttaatga tatagagttt
    15421 gtatttaatg aagatttacc aagatatata tctacaccta gctatatggc ctatttaaaa
    15481 attggagaag gatgttccaa taattgtact tattgtataa taccaaaact aagaggtaaa
    15541 tatagaagta gaaaatttga agatataatt aaagaagcta agaaattagc tgaatctgga
    15601 gtaaaagaac ttgtggttat agcacaggat acgacaaagt atggttttga tttatatgga
    15661 aaagaaagat tatcagaact tttagaagaa cttgcaaaaa tagatggatt taagtggata
    15721 agggttatgt attcatatcc agaatctata acagaagaat tgatacaagt tataaagaaa
    15781 tatgataata tatgtagtta ctttgatatg ccaatacagc atgcaagtaa caatatttta
    15841 aaattaatga atagaaaaac tactaaagaa gatatattaa ataaaatcaa tctgataaga
    15901 agtaatatac cagatgctat acttagaact acaataatag tagggtttcc tggtgaaaca
    15961 gaagatgact tcaaacaact tgtggacttt gttgaagagg ttaaatttga tagacttggt
    16021 gcatttgcat attcaagaga agaagataca ccagcagata gattgccaaa ccatatagat
    16081 gaagaagtaa aaatacaaag aagagatact ttaatgatga ttcaacaaaa aatatctgaa
    16141 gaacttaatg acaaaaagat aggaaaaact tatgaggtgt taatagaaga acagatagaa
    16201 gataatgtat atacaggaag aacacaaggt gatgcagaag aaattgacag tatagtgtat
    16261 gtaaaaagtg tagacaatct tgaagtagga gaatttgtaa gtgttcaaat aaatgatgca
    16321 atggaatacg atttaatggg agatgtttta tatgaacttg cctaacaagt taactttgtt
    16381 tagaatattt ttaataccag tatttgtttt aattatgttg ctcaatgtac caaataaatt
    16441 tttaatagct tgtattattt ttataatagc ttcaattact gatgctttag atggaaaaat
    16501 agctagaaaa tacaatctag tcactgactt tggaaaattt atggacccat tagcagataa
    16561 gttattggtt atttcagcac ttacatgtat gatagaagac catcttgtaa gtagttggat
    16621 ggtaattata atagtggcta gagagcttac agttagcata ttaagagcta tagctgctgc
    16681 tgatggaaaa gtaattgctg ctggaaacag tggaaaactt aaaacgataa ctcaaatggt
    16741 atctatagtg tttttattgt taggtgctca gtttgaaaat gtattgatat taaatattgg
    16801 tgaaattctc attcttatag ctacactact tacattatat tctggatggg aatatctata
    16861 taaaaataaa gagcttttta tgtcttctaa gtaaaaaatt atgctttaat aaataaaaac
    16921 taatgattaa taaataaaaa ataacaactt aaaattttaa aaatgtaaaa aagggtatac
    16981 tattatagta tacctctttt cattttaaga atattttctt ttgtgtatat aattattaat
    17041 aatggtttat aaataaaatg taatgaataa aaatataatc attagtagcg tttttaaatt
    17101 gactaggttt gatttatgtt atataattaa tatataacaa gagaacaaat gtttgtagat
    17161 aaatgaaggg atggtataga gatgagtgta gatcaagaaa aattaaaagc gttgaatgaa
    17221 gctttaggta aaattgaaaa agattttggt aaaggttcag taatgaaatt gggagaagca
    17281 acatctatgt ctatagatgt tatatcaaca ggagcgattg gtttagacat agctattggt
    17341 ataggaggtc tacctagagg gagaatagtt gaagtatatg gtccagaatc ttctggtaaa
    17401 actactgttg cgcttagttg tgtagcatca gctcaaaaag atggaggaat agctgcattt
    17461 atagatgcag aacatgcact tgacccagta tatgcaaaag ctttgggtgt ggatgttgat
    17521 aacctaataa tatctcaacc agatacaggt gaacaggctt tagagatagc agaggcattg
    17581 ataagaagtg gagcgataga tataatagta atagactcag tagcagcatt agttccaaag
    17641 gctgaaatag atggagatat gggtgattct catgtaggtt tacaagctag acttatgtca
    17701 caagcactta gaaagttaac tggttcaatt aaaaaatcaa attgtgttgc tatatttata
    17761 aaccagttaa gagagaaagt aggaataatg tttggaaacc cggaaactac tactggagga
    17821 cgtgcactaa aattctattc atcagttaga ttggatgtta gaaaaataga tacaataaaa
    17881 caaggtgata aagttatagg tagtagaact agagttaaag ttgttaaaaa caaagtagca
    17941 ccaccattta agcaagctga atttgatata atgtatggag aaggaatttc aaaaattgga
    18001 gaccttttag atatagctgc tgatgtagat atagttaaaa aatcaggttc atggtatagt
    18061 tacaatgata ctaaacttgg acaaggaaga gaaaatgtta aaaaattctt ggaagataat
    18121 ttagatttaa ctactgaaat agatgagaaa gttagagcat tttacaattt aaatgaagaa
    18181 catgaagaat caggtatttc agtatcaaaa gaaattgtag aagaataagt aaaaatacaa
    18241 aaaaataact agtaattaaa gacatgccac gctccctatt agcttgacac aatgttgaaa
    18301 aaattataaa attaagtgag gataaaatat ctaaatttaa ttttaattat taggaatatt
    18361 ttttaatcat agtgatgaca ttttgggatt attaacgaag gaggtggatg tcatagatag
    18421 tgtagtaatg attgtaatag gtgcagctgt tggtattata gctggatatt ttgttagaaa
    18481 aaatatatct gaagcaaaaa ttggacaagc tgaaaacttg gccaaagaaa taatagataa
    18541 agcacaccac gactcagaaa cagtacaaaa agaaaaatta ctagaagcta aagaagagat
    18601 ccataagtgg agaacagaag ctgaaagaga aaacagagaa agaagaacag aagtacaaaa
    18661 gtatgaaaaa agagtagtac aaaaagaaga agtcttagat agaaaattac aaaatttaga
    18721 atctaaagaa gtaaacctaa gtgaaaaatt aaaggtagtg gaaaagaaag aagaagaagt
    18781 tgaagtaata aaaactcaac agttagaaaa attagaaagt atatcaggaa ttacttctga
    18841 taaggccaaa gaaatcatct taaccaatgc ggagagagat gtaagacgtg aaatgtctat
    18901 aatgataaaa gagatagaaa gtcaagctaa agaagaagct gataagaaat caagagaaat
    18961 tataggatat gcaattcaaa aatgtgcagc agatcacgta gccgaaacta ctgtaacagt
    19021 agtaagcttg cctaatgatg aaatgaaagg tagaataata ggtagggaag gtagaaatat
    19081 aaggacttta gaaaccttaa caggtataga ccttataata gatgacactc cagaagccgt
    19141 aattttatca ggatttgatc caataagaag agaaattgca agaattgctc tagaaaaatt
    19201 aatagcagat ggaagaatac atccagctag aattgaagaa atggttgaaa aggcgagaaa
    19261 agaagttgac aatattataa aagaatatgg tgaacaagct gcatttgaaa ctagtgtaca
    19321 tgggattcat ccggaattaa taagattatt aggtagactt aactatagaa ctagttatgg
    19381 acaaaatgta cttaaacatt ctatagaagt tgctcatata gctggtataa tggctgctga
    19441 aataggagca gatattagat tggctaagag ggcaggttta ttacatgata taggtaaggc
    19501 tgtagaccat gaaatggaag gtacacatgt tgaaataggt atggatttac ttagacgtta
    19561 taaagagtct aaagaagtta tacatgccat gagtactcat catggagatt acgagcctca
    19621 aacagtagag gctgtattag ttacagcagc agatgcaata tcagctgcta gacctggtgc
    19681 gagaagagaa actttagaag catatataaa gagacttgaa aaattagaag aaatagctaa
    19741 ttcatatgaa ggtgtagaaa aatcatttgc tatacaagct ggtagagaaa taagaataat
    19801 ggtaaaacca gaagctataa atgatgaaga aatacatcta ttggctagag atatgactaa
    19861 gaaaatagag gatgaacttg aatatcctgg acaaataaaa gtaagcataa taagagaaac
    19921 aagagctata gagtatgcga agtaaaagaa ataatatcta aataataaga aaatattttt
    19981 agaaatttaa atttgccaca agatatgata atatatcttg tggcttttaa attgtttaag
    20041 tttaatttta tataatgcaa aaataatcta agtgctaaaa gtgctactta caaaagatta
    20101 ataaaataaa gctttatata tgtattattt atataattat ccaagtttgt ttgatataat
    20161 gaagttgtaa aatcaatact ttactacaaa ataaatagta aatactacag taaaatctaa
    20221 aaataggata taatagtatt aatataagaa gattataaat aaaacctgct aatgaaaata
    20281 gattattttt agtaggtttt taaatttttt ataataaatc ttatttttgg ctaacaagat
    20341 ttattttttt taagacttta cttattattt taagcattaa ttatgaaatt tatca
//
LOCUS       DAEPIN010000066        20384 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.066, whole genome shotgun sequence.
ACCESSION   DAEPIN010000066 DAEPIN010000000
VERSION     DAEPIN010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20384
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.066"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            655..1563
                     /gene="trxB"
                     /locus_tag="KR250_002853"
     CDS             655..1563
                     /gene="trxB"
                     /locus_tag="KR250_002853"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HBH0068768.1"
                     /translation="MVDIIVIGAGPAGLTSAIYAMRAGLSVTVFEKNIYGGQVASTSE
                     VENYPAVQKISGVEFSNNIYNQAVAQGVDIQFDEVEEINLEGKVKVVKTSSGEHKAKA
                     VILANGVERRKLGCAGEQEFTGRGVSYCATCDGAFFKDKEVAIVGGGNTALEDALFLA
                     NNCTKVYLIHRRDSFRGEEVLEKSVKARENIEILYSHGVEKIEGEKTVSKIEVKNLKT
                     EEKRTIDVSGIFIAIGLKPNNKMFENVLDLDEGGYIISDESCTTSVEGVYVAGDSRTK
                     FLRQIITAASDGAIAAVQAANYINVE"
     gene            complement(1653..3476)
                     /gene="typA"
                     /locus_tag="KR250_002854"
     CDS             complement(1653..3476)
                     /gene="typA"
                     /locus_tag="KR250_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HBH0068769.1"
                     /translation="MSQKHKIINIAVIAHVDAGKSTLVDAFLSQSGVFRKNEVVKDCV
                     MDSNDLEKERGITIYSKNCAINYEDYKINIVDTPGHSDFSSEVERVMKTVDTVILLVD
                     ASEGPMPQTRFVLQKSLEFGLKPILFINKIDKKDQRAEEVVNEVFDLFVDLNATDEQC
                     EFPIIYGIAKQGIAKLEMDDDSEDLSPLFKTIVNHVEAYPNYDNEPLQFQISALAYDD
                     YVGRLGIGRIYKGTLKNNTQVAICREDSVVSKGKVSKLSVYEGLKQVEVDEATSGEIV
                     VIAGIPDISIGETICDLDSPLPMEMIKIEEPTLSMNFLVNDSPFVGKSGKFVTTRHLK
                     DRLEKELEVNVGLKVEPLDTTDGYKVSGRGELHLSILLENMRREGYEVGVSKPEVLMH
                     KEDGKLMEPIERVVVNCPEVYSGTIINELNMRKGMMESMSIEGDYVKIEFLAPTRGLL
                     GYRSEFINATRGEGTLVRSFEKFEEFKGEIPSRGNGVLIAQGPGVTMGYSLNALSDRA
                     VMFVDPGVEVYEGMIIGMNSRKDDMVVNPCKNKKMSNVRASGSDDAIKLSPPRIFTLE
                     EALEFIEDDELVEITPDSIRLRKRFLNEHDRLRYNKSRQGK"
     gene            3688..3930
                     /locus_tag="KR250_002855"
     CDS             3688..3930
                     /locus_tag="KR250_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0068770.1"
                     /translation="MLEVTKIIIKYFASESDTFVKEEVCNFEELLKWSHKFIEGKKIE
                     DVLYIKSKENYKVNVIKSLEIKELEIVRKQQIPPYM"
     gene            4078..4269
                     /locus_tag="KR250_002856"
     CDS             4078..4269
                     /locus_tag="KR250_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1450 domain-containing protein"
                     /protein_id="HBH0068771.1"
                     /translation="MIRVCPNCSNVDVNKLKTLVGEENVKTGCIGQCRAFKKEAVGII
                     DNELEIKETEAEFFEACKE"
     gene            4560..7061
                     /locus_tag="KR250_002857"
     CDS             4560..7061
                     /locus_tag="KR250_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HBH0068772.1"
                     /translation="MSSNKVVESYKITGMTCAACAKAVERVTKKMDGVYDQNVNIATE
                     KLKIEYDNSKVNFDDIKQVVEKAGYGIIKEESNKKIDMKIDGMTCAACAKAVERVVKK
                     LDGVESISVNIATDKANIDYDPSKVKLSQIKAAIEKAGYKPIEEVRNKVDVDEDKLRK
                     EREMKSLFVKFIVAIVFAVPLFYIAMGPMIIKPIGPWPLPEIINPMTNTFNYALIQLI
                     LVIPVMIAGYKFYINGFKSLFSLSPNMDSLVAIGTLAAFLYSLYTTLQIANGQIQGMH
                     HHQLYYESAGIIIALILLGKYLESKSKGKTSEAIKKLMGLQPKTAIVLVDGKEVETPI
                     EEVEIGDILLVKPGTKIPVDGVVIEGYTSVDESMLTGESIPVEKNVGSKVTGASINKN
                     GVIKFKAEKIGGDTALAQIIKLVEDAQGTKAPIAKLADTVSGYFVPIVIAIAVVASLL
                     WFLIGGKDIVFVLTIFISVLVIACPCALGLATPTAIMVGTGKGAENGILIKGGEALES
                     AHKVNTVIFDKTGTITEGKPKVTDIVLNNNVKEEYLIKIASSAEKGSEHPLGEAIVKY
                     GEEKNIKFEKVDNFKAIPGAGIQVTINDESILLGNRKLMNDNNIKLGDLEEKSNILAS
                     QGKTPMYIAVDGNLSGIIAVADVVKESSKKAIEILHDMGIKVAMVTGDNAKTANAIAN
                     QVGIDMVLAEVLPEDKSKEVEKLQNQGKFVAMVGDGINDAPALAKADIGIAIGSGTDV
                     AIESADIVLMKSDLIDVPTAIKLSHETIKNIKQNLFWAFGYNTIGIPVAAGILYVFGG
                     PLLNPMIAAAAMSLSSVSVVSNALRLKNFKAYKRD"
     gene            7221..7889
                     /locus_tag="KR250_002858"
     CDS             7221..7889
                     /locus_tag="KR250_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0068773.1"
                     /translation="MNSSILVIEDDSNIQELISEFLSAEGYQVDTANDGLEGIQKFKQ
                     GSYDLVILDIMMPNLDGYGVCKMIRKSSSVPIIFLTALNDEGDQLKGFDLECDDYITK
                     PFSFNLLIKRVEAILRRSNKTINDKFIVFEKLKLDLNTYIAEIDGEPIELTLKEFNIL
                     KALIEKYPQVITREGLLDSIWGYDYYGDTRIVDAHIKNIRKKISLPYIKTVKGIGYTL
                     EKDI"
     gene            7873..9150
                     /locus_tag="KR250_002859"
     CDS             7873..9150
                     /locus_tag="KR250_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HBH0068774.1"
                     /translation="MRRIFDKWEKLSIKYKLFSITTSLLIALALIIYLILYFLLPSYY
                     HEYKIESLQESLKSLVDSSIHFDTYTLEERLYYMAKDQNLAILLKDNQGKIVYGKNEV
                     VILRYSKYMINSLEDEYRTSIPIYTKDAKDGPYTLELVMPLQPIDEANEVIRKLMPYI
                     ISIAILIAIIGAYIYSIVITKPLINIIESEREQEYRRKDFVATISHELKTPITIISGQ
                     IEGMIYSVGKYKDRDTYLKKSYECTQELKDLVNEMIEVSKSEILEKDLKLVSINISEL
                     LNRLVKRQVFLIEEKHMKTILKIEENLEIKADQERITKAINNIINNAIKYSPEESELI
                     IRLYDKNKRISKKTSNQRVVLEIENTGVTIEKRYLEEIFNPFYRIEKSRSRKTGGSGL
                     GLYIVSQIFKSHGFDYSIKNKENSVVFTVEFKN"
     gene            9551..9772
                     /locus_tag="KR250_002860"
     CDS             9551..9772
                     /locus_tag="KR250_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0068775.1"
                     /translation="MDRLKRDMKNMKEDLKEGVKKTAYVMKNTAEDLKDDAQGAMVNL
                     EMKKDEMMERYEQKKFSKELEKQMKNEMK"
     gene            complement(10061..10825)
                     /locus_tag="KR250_002861"
     CDS             complement(10061..10825)
                     /locus_tag="KR250_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBH0068776.1"
                     /translation="MFAEERIQAILNILKKEGRVTVKELSSKFNVTEDCIRKDLKNIE
                     KISSIRRIYGGAVLARETLENQDTKDRKEINIPTKKIIAEKAFNLIEDRETIFLDIST
                     INILLAKLLAESNKKVTLVTNMLDILNVVTSKPNNLNIISTGGLLNLSLDGFVGTPTI
                     EFISKYKFDKTFMGSCGVDVFNSSLTTFEIEDGLTKKAIINSSKKVFIVMEDKKFKFD
                     GNFKFAHLEDISSIITEKTPTPDIVDILSEFNVNVL"
     gene            11099..11773
                     /gene="fsa"
                     /locus_tag="KR250_002862"
     CDS             11099..11773
                     /gene="fsa"
                     /locus_tag="KR250_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HBH0068777.1"
                     /translation="MQLILDTGNVEEIKELCTCLPIDGVTTNPSIVSKEKKNFKQLIN
                     EIGEIIGEDMPIHAQVLSTKYEEILEEALYISSLRKNIYVKIPVTQDGLRAIKDLHRK
                     GVKITATAIFTAHQGFLAAKAGANYIAPYVNRLDNISGDGVAMVSELIKIIDTYKMDT
                     KVLAASFKNAQQVIELMQHGVHSVTVPYDICKSMMNHPLTDWSVDKFIEDWENTFGKG
                     SKTNNI"
     gene            complement(11895..13289)
                     /locus_tag="KR250_002863"
     CDS             complement(11895..13289)
                     /locus_tag="KR250_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HBH0068778.1"
                     /translation="MGIILKPKQFYMTVIATIILYFSLNYSFNIKFSICLSFLTILIW
                     AVDSVEKTFVALFFVILAFIFNIAPIKVILQFLFTENFYIIILAYIITNAVTKTGVAK
                     IISEKLILNTVNTPKKMILLSYILGILLIFFIPQPFPRVILVSAFYKEFLKEQQITED
                     SKSILLFSIFTASTFTSMFFVNGDTLLNYVVLELGNCNINWGQWAFYMSVPTIITCFI
                     TYFLFTFVFKKELHSIKFISNENLEGNKLHFSYSNLKKEFNLLNKEQKHVFLCLGIMF
                     FMFLTQFIHNINTLIIMSICVLLLLLRKIVGFSTLKEINWKVLIFFIAAFSIGGVLKY
                     SGVVDIMGNYLIKFIPNSSEIISILFLITLTIILNICLGSAVTTSSVVIPLLGSLHIL
                     KENSVTLCLFVYIIVSIQYILPFHHATIMVGHGENLYNSKVIFKYGLTLIPLTYFIII
                     CITFPWWKFIGLEI"
     gene            complement(13367..14713)
                     /locus_tag="KR250_002864"
     CDS             complement(13367..14713)
                     /locus_tag="KR250_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBH0068779.1"
                     /translation="MKDLTTGHEGKSIFFFAMPMLIGSLFQQLYNTADSIIVGRFIGK
                     EAMAAVSGANPIMFLLVAALMGVSLGFSILVSQFYGSGDLKKVKATIDTTYILLFIGS
                     ILISILGIVFGGPMLKLMNTPESVFAQSKLYLTIIFSGILFSAGYNSVSAILRGLGDS
                     VTPLYFLIIATILNIVLDLTFIVVLRMGVEGVALATIMAQAVSFIISIIYLNKKHEVL
                     KFKIKGIVYDNKIFKDGLRLGLPSGVQQMLFSIGNMALQFLVNSYGTSAMAAFGAGLR
                     IENFISLPIMNLGSAVSTFVAQNIGAGENERVKKGIRESIKMTLVLAVTVIALILLFR
                     ENLIALFNTDKDVIKIGSSYLFIIGPFFLFIGTSFVLSSAMKGAGDSMFALISSIVSL
                     WLGRLPASYMLSKFFGTDGIWMGIPFGWTLGLIVTVIYYKKGHWKTKAIVNHRINE"
     regulatory      14963..15065
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            15159..16535
                     /locus_tag="KR250_002865"
     CDS             15159..16535
                     /locus_tag="KR250_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HBH0068780.1"
                     /translation="MQTTNSSTMQKLFPILTNKNVDMKKEIIAGVTTFLTMAYIIAVN
                     PNILSETGMPAGALVTGTCLAAAFGCILMGVVANLPFALASGMGLNAFFAYTVVLQMG
                     VPWEVALTAVFVEGIIFIVLSVSGVREAVVNAIPKNMKLAVTGGIGIFIALIGLVNSG
                     IVIGDQATLIKMGRFTPAVIITCVGLIIIAVLDKKRVKGSILFGIVVSSLLAWAFAFI
                     NPEYAQKLGIYLPGGLFKFESLAPIAGKIDLGYVLHPTNIGGFLVVVCTFLFVDFFDT
                     VGTLVGVCSKANMLDEKGNVPNVGRALLTDAVATTVGAGLGVSTVTTYVESSTGVIAG
                     GRTGWTAITVGILFLAAMFFSPVFIAIPSCATAPALIYVGYLMLGTVKDIEFDNITEG
                     VPAFVTIACMALTYSIGDGLTLGILTYVFVNIFYNIFGAKKAEDKKHVSVVMIVLAIL
                     FIIKLWLL"
     gene            complement(16649..17794)
                     /locus_tag="KR250_002866"
     CDS             complement(16649..17794)
                     /locus_tag="KR250_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBH0068781.1"
                     /translation="MNTFEQLKISSTLIDGLKKQDITSPTEVQSLVIGNIIQNKDLLI
                     NSQTGTGKTLAYLLPIFEKIDTSKRETQALILAPTHELVMQITNQVELLAKNAELSVT
                     SLALIGEVNIQKQIKNIKAVKPHIVIGSCGRVLDLIKQKKLKSHNIKTIVLDEVDNLL
                     NGKNITCIEDIIRTTLRDRQIIGCSASLTDSTIKICDKLMKEFEIIKTKEKSQINPNI
                     NHSYLLGEIRDKFTFLRKALAATNPKKAIVFVNNEKNIEVLVSKLNYHNYKAIGIFGN
                     MEKEDRKNAINKFKLGKAKILITTDLSARGLDIVDVSHVFNLDFPKSKNEYLHRCGRT
                     ARGNRSGNTISIITKKELDIIKDLQKEFNIVITPKTLQNGELIDIIK"
     gene            18047..18754
                     /locus_tag="KR250_002867"
     CDS             18047..18754
                     /locus_tag="KR250_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0068782.1"
                     /translation="MLKVINVSKSYKNKKVVSSVSFDVNDGELFGFIGHNGAGKTTTI
                     KAIVGIHDFEEGEVLINSKSIKKQPVECKKEMAYIPDNPDLYESLTGIQYLNFIADIF
                     EVAKDKREELIKYYSNKFELNKALGDLISSYSHGMKQKLAIISALIHSPKILILDEPF
                     VGLDPKASFILKEIMREFCDKGGCIFFSTHVLEVAEKICDKIAIIKDGNIIAYGTTEE
                     VKGSNSLESIFMELIEK"
     gene            18801..>20384
                     /locus_tag="KR250_002868"
     CDS             18801..>20384
                     /locus_tag="KR250_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0068783.1"
                     /translation="MSNLSILIKTNIINEFKLNALKKADGREKQKIMGMMLSVVIIAG
                     LLIFYVTSLSLSLVDILKQINQLEMLLIFGFGLSTLTTIVTSLYKTSSYLFQAKDLDL
                     LTSLPIKESTVLASKILMLVGANYLFSSICMLIPAVVYFLNSDVSITYFPYLILLFLA
                     LPLLPIVASSIIFLFIGQISSRIKYKNLVLIIGSIILMLLYTLLMSRLNSMSVEMLKN
                     SVSISETIRKVYPPIYYFVDALKTGSIVSFLAFIAISIIPFFMFVTLFAKKFKDINAK
                     MSEGYKSKNYEFKGQKTSRPSKALLKKEFKRYTSSFIYFLNSSAGLLFLIVGTILILL
                     FGADKVGELLKLNIDFNLIKIQLLGVFFFIIVMNCTTYCSISLEGKNLWILKSSPIDE
                     REIFWSKILMNFLLNAPLSILCLILVSIRLDFEIQFIIVSICLIVSFSMFVALMGLLS
                     NLLYPNLDWKNEVAVVKRSASMIVTMLVSLAYVIILGGIYVLLGISFLNIYIILASIL
                     TLILDFMIWRIISTKGVRIF"
BASE COUNT         7755 a         2449 c         3166 g         7014 t
ORIGIN      
        1 gtttaaaaac ctagattttc atagtgatga agctgttttt aatgaaagta atcaaaagat
       61 aactattaaa tatctgtaat attacaattt tatactacac tatattacaa aaaaagttta
      121 caaaacttta atttgaaaaa tgtaattata tggtatatca tataaatata agtaaatagt
      181 gctaatattt aaactattaa tcataattgg ttcaacaaaa gtatatagtt aataatgttt
      241 aatttgaaga aattgttagt gtgaaattac atatatattc aaattaaatt cataaatatt
      301 tcacataata atagtatact gtaaacaaaa ggaggtatta aatatgggaa tagtacttat
      361 aacagcagca ttagttttgg ttatgtatgg actttcaatt ctatcatata tatcagaaag
      421 caatatatta gaagacactg taaatgataa ccaataaggt tttgaattat ataaataagt
      481 gagaactgta aataaaagtt aatttttatt tgcagttttt ttatatatat gtaaaataaa
      541 gttaattaat aaaaaataat gttttgttta tagtatatat ggataataat agtaaagtat
      601 aagaatgatg agaataatat ataagaaata ataatataaa gagaggtaaa cgttatggta
      661 gatatcattg taattggtgc tggaccagca ggtttaactt cagcaattta tgctatgaga
      721 gcagggttaa gcgttacagt atttgaaaaa aatatttatg gaggtcaagt tgcaagtacc
      781 tctgaggtag aaaattatcc agcagttcaa aagatttctg gggtagaatt ttctaataat
      841 atatacaatc aagcagttgc tcaaggtgtt gatattcagt ttgatgaggt tgaagagata
      901 aatttagagg gaaaagtaaa ggttgtaaaa acttcttcag gtgaacataa agcaaaggct
      961 gttattcttg caaatggagt tgaaagacgt aaattaggtt gtgcagggga acaagaattt
     1021 acaggtagag gagtatctta ctgtgcaaca tgtgatggag ccttttttaa agataaagaa
     1081 gttgctatag taggtggagg aaatactgca ctagaagatg ctttattttt agctaataat
     1141 tgtacaaaag tgtatttaat tcacagaaga gatagtttta gaggtgaaga agtgttggag
     1201 aaatctgtga aagcaagaga gaatattgag atactttaca gtcatggtgt agaaaaaata
     1261 gaaggagaaa agactgtatc gaaaatagaa gtaaaaaact taaagactga agaaaaaaga
     1321 actatagatg ttagtggtat ttttattgct ataggtctta aacctaataa taaaatgttt
     1381 gaaaatgttc ttgatttgga tgaaggtgga tatataattt cagatgagtc ttgtacaaca
     1441 tcagtagaag gtgtatatgt agcaggagat agtagaacta agtttttacg tcaaataata
     1501 acagcagcat cagatggagc tattgcagcc gttcaagcag ctaattatat aaatgttgaa
     1561 taatataaat ttcaaataaa aatagccata taaactgaaa taatttttca gtactatatg
     1621 gctattttgt taattagagg taaatgtacg ttttatttac cttgtctaga tttgttatat
     1681 cttaatctat catgttcatt taagaatctt tttctaagtc ttatagaatc aggtgtaatt
     1741 tcaactaatt catcatcttc tataaactct agagcttctt caagagtaaa tattctagga
     1801 ggagataatt ttatagcatc atctgaacca gaagcacgta cgttactcat tttcttgttt
     1861 ttacaaggat tgactaccat atcatctttt ctggaattca taccaattat cataccttcg
     1921 taaacttcta caccagggtc tacaaacatt acagctctat cacttaaggc attaagagaa
     1981 taccccatag taacaccagg tccttgtgct attaaaacac catttcctct tgaaggtatt
     2041 tctcctttaa attcttcaaa tttttcaaat gaacgaacta aagtaccttc tccacgagta
     2101 gcgtttataa attcacttcg atatcctaaa agaccacgag taggtgctaa gaactctatt
     2161 tttacatagt ctccttctat tgacatagac tccatcatac cttttctcat gtttaattca
     2221 tttataatag taccagagta aacttcagga cagtttacaa caactctttc tattggttcc
     2281 attagttttc catcttcttt atgcattaaa acttcaggtt tagaaacacc aacttcataa
     2341 ccttcacgtc tcatattttc aagtagtata gataagtgaa gctctccacg tccagaaact
     2401 ttgtatccat cagtagtatc aagaggttca actttaagac caacatttac ttcaagttct
     2461 ttttcaagtc tatctttcaa atgtctagtt gtaacgaatt ttccactttt tccaacgaat
     2521 ggagagtcgt ttactaagaa gttcatagaa agagtaggct cttctatttt tatcatttcc
     2581 ataggtagtg gactatctaa atcgcatata gtttcaccta tagatatatc aggaatacca
     2641 gctataacaa ctatttctcc actagtagct tcatcaactt caacttgttt taaaccttca
     2701 tacacagaaa gttttgatac ttttccttta gatacaacag aatcttctct acatatagca
     2761 acttgtgtat tgtttttaag agtacctttg taaattctac ctataccaag tctacctaca
     2821 taatcatcat atgcaagagc tgatatttgg aactgtagag gttcattatc gtaattagga
     2881 taagcttcaa cgtgatttac aatagtttta aatagaggag aaaggtcttc actatcatca
     2941 tccatttcaa gtttagcaat accttgttta gcaataccat atattatagg aaattcgcat
     3001 tgctcatctg ttgcatttaa gtctacaaat aaatcgaaaa cttcattaac tacttcttcc
     3061 gctctctggt cttttttatc aatcttgttt ataaataata ttggttttaa accgaactct
     3121 aaagattttt gtaaaacgaa tctagtttga ggcattgggc cttcactagc atcaactaat
     3181 agaataactg tgtcaacagt tttcataaca cgttcaactt cagaagaaaa gtcactgtgt
     3241 cctggagtat caactatgtt tattttgtaa tcttcatagt ttattgcaca gttttttgaa
     3301 tatatagtta tgcctctttc tttttcaagg tcgttactgt ccataacgca gtctttaact
     3361 acttcgttct ttctaaaaac accgctttga gaaaggaaag catcaaccaa tgttgatttt
     3421 ccagcgtcaa cgtgggctat aactgcgata tttataattt tatgtttttg tgacattttt
     3481 gttgctccct tcgattaaaa taacttatta attatagcac aatatattat taggtacaag
     3541 gaggaatata ttaaattgta ttaatatagt ttaaattgga taaaactgtt agttttgcat
     3601 ctgaaaatta ttctgtttaa aaaaattttt tagacaaata ataaccttaa tagataaaaa
     3661 gattaataaa agagtaaagg atgatatatg ttggaagtaa ccaaaataat aattaaatat
     3721 tttgcaagtg aaagtgatac ttttgtaaaa gaagaagttt gtaattttga agaacttctt
     3781 aagtggtctc ataaatttat tgaaggtaaa aagatagaag atgttttgta tataaagtct
     3841 aaagaaaact ataaagtaaa tgtgattaag tctttagaaa ttaaggaatt ggaaatagtg
     3901 agaaaacaac agataccacc gtacatgtag tattaataat atataaaatt aatatgtaat
     3961 actcgcaata ggatataaat ggtatatatg gtaataattt atattaaaaa aacttttata
     4021 aagtttatat ttgggtataa aagtattaat tagacattaa ttaggaggaa cttaaatatg
     4081 ataagagtat gtccaaactg ttcaaatgtg gatgtaaata agctaaaaac tttggttgga
     4141 gaagaaaatg taaagactgg ttgtataggt cagtgtagag catttaaaaa agaagctgtt
     4201 ggaataatag acaatgagct tgaaataaag gaaacagaag cagagttttt tgaagcatgt
     4261 aaagaataaa ttttaaataa aatataaatt ataataaata gagcatctaa attgtataag
     4321 cattaaacaa tttagatgct ctatttttat atatacattt aattatgagt atacttttct
     4381 attaaataca aataaatata actaatacat ttatttatta aaaaaatatt gatttaatat
     4441 acctataggg ggtataatga aaatatagat ttctaaataa gtaatttatc aatattacat
     4501 atcaaaataa aacgttacaa attagctgaa aatatattga aagaaaggaa gattttatta
     4561 tgagttcaaa taaagtggta gaaagttata aaattacagg tatgacatgt gcagcttgtg
     4621 caaaggcagt agagagagta actaagaaga tggatggagt atatgaccaa aatgtaaata
     4681 tagctactga aaaacttaaa atagaatatg ataattctaa ggttaatttt gatgatataa
     4741 agcaggttgt agaaaaagca ggatatggaa ttataaagga agaaagtaat aaaaaaattg
     4801 atatgaaaat tgatggaatg acatgtgcag cttgtgcaaa agctgtagag agagtggtaa
     4861 aaaaattgga tggagtagaa agtattagtg tgaatattgc aacagataaa gctaatatag
     4921 actatgaccc atcaaaagta aaattatctc aaataaaagc agctattgaa aaagctggat
     4981 ataaaccaat agaagaggta agaaataagg ttgatgtgga tgaagataaa ttgagaaaag
     5041 aaagagaaat gaagtcttta tttgttaaat ttatagttgc aatagtattt gcagtacctc
     5101 tgttttatat agcaatggga cctatgataa taaagcctat agggccatgg cctttgccag
     5161 aaattataaa tcctatgaca aatactttta attatgcatt aattcagtta attttagtaa
     5221 taccagtaat gatagctggt tataaatttt atataaatgg gtttaaatca cttttttctt
     5281 taagccctaa tatggattct ttagttgcaa taggaacatt agccgcattt ttatatagtc
     5341 tatatacgac actacaaata gccaatggac aaattcaagg tatgcaccat catcagttgt
     5401 attatgaaag tgcaggaata ataatagcac ttatcttact tggtaaatat ttagaatcaa
     5461 aatcaaaagg aaaaacatca gaagcaatta aaaagcttat ggggctacaa cctaaaactg
     5521 caatagtgtt agttgatggt aaagaagttg aaactcctat agaagaagta gagataggag
     5581 atatactatt agtaaaacca ggaactaaaa tacccgtgga tggagttgta atagaaggat
     5641 atacatcagt tgatgaatca atgcttacag gtgaaagtat accagtagaa aagaatgttg
     5701 gaagtaaggt gacaggagct agtattaata aaaatggtgt tattaagttt aaggctgaaa
     5761 aaattggtgg agatactgct cttgcccaaa taataaagtt agttgaagat gcacaaggta
     5821 caaaagcacc tattgctaaa cttgccgata ctgtatctgg atattttgta ccaatagtaa
     5881 ttgcaatagc tgtagttgca tcattattgt ggtttttaat aggaggaaaa gatatagtat
     5941 ttgtacttac tatatttata tcagtacttg taattgcttg tccttgtgca ttaggattag
     6001 caacaccaac tgctataatg gttgggactg gaaagggcgc agaaaatgga atcttgataa
     6061 aaggtggaga ggcactagaa tctgcacata aagtaaatac agtgatattt gataagacag
     6121 gtactataac agaaggaaag ccaaaagtaa cagatatagt attaaataat aatgtaaaag
     6181 aagaatatct tatcaagatt gcttcaagtg cagaaaaagg ttcagaacat cctcttggag
     6241 aagcaatagt taagtatgga gaagagaaga atataaaatt tgaaaaagtt gataatttta
     6301 aagctatacc aggtgctggt atacaagtta ctattaatga tgaaagtata ttacttggaa
     6361 atagaaagct tatgaatgac aataatataa agcttggaga tttagaagaa aaatcaaata
     6421 tattggcaag ccaaggtaaa acacctatgt atatagctgt agatggaaat ttatcaggta
     6481 taatagcagt tgcagatgta gtcaaagaaa gtagtaaaaa agcaatagaa atacttcatg
     6541 atatgggaat aaaagttgca atggtaactg gagataatgc gaaaactgca aatgctatag
     6601 caaatcaagt cggtatagat atggttttgg cagaggtttt accagaagat aagtcaaaag
     6661 aagttgaaaa actacaaaat caaggaaagt ttgttgcaat ggtaggggat ggaataaatg
     6721 atgccccagc tcttgcaaaa gcagatatag ggatagcaat aggaagtgga acggatgtag
     6781 ccattgaatc tgcggatata gtacttatga agagtgattt aattgatgtt ccaacagcaa
     6841 ttaaacttag tcatgaaact ataaaaaata taaaacaaaa cctattttgg gcatttggat
     6901 ataatacaat tggtatacct gtagcagcag gaatattata tgtatttgga ggacctttat
     6961 taaacccaat gattgcagca gcagcaatga gcttaagttc agtttcagtc gtatcaaatg
     7021 cacttagact taaaaacttc aaggcatata agagagatta gattaataaa ttaaataaaa
     7081 tgtatccaaa agagctatct ttaaattaaa aggattaatt tacaaattaa gatagctctt
     7141 ttttcatgta aaattcacaa caataagata aaatataagt aatatacgaa attttaaata
     7201 aaatagaaag gattgataga atgaattcgt caatacttgt aattgaagat gattcaaata
     7261 tacaagagtt aatatcagaa tttttaagtg cagaaggtta tcaagttgat acagcaaatg
     7321 atgggttgga aggtatacaa aagtttaagc aaggaagtta tgatttagta atattagata
     7381 taatgatgcc aaacttagat ggttatggag tttgtaaaat gataagaaag tcatcaagcg
     7441 ttccaattat atttttaact gctttaaatg atgaaggaga ccaacttaaa gggtttgact
     7501 tagaatgtga tgattatata acaaaaccat tttcatttaa tcttcttata aaaagagtag
     7561 aagcaatttt aaggcgaagt aacaagacta taaatgacaa atttatagtt tttgaaaaat
     7621 taaaattaga tttaaacaca tatattgcag agatagatgg agaacctata gaacttacac
     7681 taaaagagtt taatatatta aaagctttga tagaaaagta tccacaagtt ataactaggg
     7741 aaggtctttt ggatagtata tggggttatg actattatgg agatactagg attgttgatg
     7801 cacatataaa aaatataaga aaaaaaatat cattaccata tattaaaact gtaaaaggaa
     7861 taggatatac acttgagaag gatatttgat aagtgggaaa agttaagtat aaagtataag
     7921 ttattttcaa taacgacatc attattaata gcattagctt taataatata tctaatttta
     7981 tattttttat tgccatcata ttatcatgag tataaaattg agtctcttca agaaagtttg
     8041 aaatctttag tagatagttc aattcatttc gatacatata ctttagaaga aagactttat
     8101 tatatggcta aagaccaaaa tttggctatt ttattaaaag ataaccaagg taaaattgta
     8161 tatggtaaga atgaagtagt tattttaaga tatagtaagt acatgattaa tagtcttgag
     8221 gatgagtata gaacttctat acctatatat actaaagatg caaaagatgg tccatatact
     8281 ttagaactcg ttatgccatt acaaccaatt gacgaagcaa acgaagttat aagaaaacta
     8341 atgccataca tcatatcaat agcaatattg atagcgatta ttggagcata tatatattca
     8401 attgtaataa caaagcctct tataaacata atagagagtg agagagaaca ggaatataga
     8461 aggaaggatt ttgttgctac aatatcacat gaactaaaaa ctccaataac cataataagt
     8521 ggtcaaattg aaggtatgat ttatagtgta ggtaagtata aggataggga tacttatctg
     8581 aaaaaatcgt atgagtgtac acaagaatta aaagatttag taaatgaaat gatagaggtt
     8641 tcaaaatcag aaatacttga aaaagatttg aaattggtat caataaatat aagtgaatta
     8701 ttaaatagac ttgtaaagag acaagtattt ttaatagaag aaaaacatat gaaaactatt
     8761 ttaaaaatag aagaaaattt ggaaattaaa gcagaccaag agagaataac taaggcaatc
     8821 aataatataa taaataatgc cataaaatat tctcctgaag aatcagagct tataattaga
     8881 ctctatgata aaaataaacg aataagtaaa aagacttcta atcaaagagt ggttttagaa
     8941 attgaaaata ctggtgtaac tatcgagaaa cgttatttag aagaaatatt taatccattt
     9001 tatagaatag aaaaatctcg ttcaagaaaa actggaggaa gtggacttgg actttatata
     9061 gtcagccaaa tttttaaaag tcatgggttt gattatagca taaaaaacaa agaaaactct
     9121 gtagtattca cagtagaatt taaaaattaa ttacttatta taaagaatat ataactttca
     9181 ataatagttc attttaaaat cacaaaaaat tcataatgaa actgtattat ataaatataa
     9241 agaagcttaa aaccataaaa tacaccaact ataatgatga gatttcctta catcctccat
     9301 ataagtcatc attatagtaa cccaacaatt aagttactat gaatttaaaa aataacaccg
     9361 agcatataat ctatgcttag tgttattttt attttgaaat aaaaaatatt taatttattg
     9421 aaatgtatag atttagatag tttaaactat atttaagact taaaaattat aattgatatt
     9481 acaaataaat atatgtactt tgggaaaaat aaatatagga agtattatat ttacagagga
     9541 ggtagttttg atggatagat taaaaagaga tatgaaaaat atgaaagaag atttaaaaga
     9601 aggtgttaaa aagacagctt atgttatgaa aaatacagct gaagatttga aagatgatgc
     9661 acaaggtgct atggtaaact tagaaatgaa aaaagatgag atgatggaaa gatatgaaca
     9721 aaagaaattt tctaaagaat tagagaagca aatgaagaat gaaatgaaat aagcttttat
     9781 ttatgaataa caaattattg tattcatata aaaagtagcc agtaatatta aatagtttat
     9841 ttaaggaaat agactttcta aaaataactg tattagattt agaaagtcta tttcaaatta
     9901 gttttataaa tagacataag gataaagtag atgttataga actagatttt aaaaaataga
     9961 tactaaggat aaaataaata ttatagaaat atgtttttaa gaatatttat caaataaaaa
    10021 tttatattta taagaaatcc tataaaacat ataattaaac ttataaaaca tttacattaa
    10081 attctgataa aatatctact atatcaggag taggcgtttt ttctgtaatt atagaactaa
    10141 tatcttctaa atgagcaaac ttgaaatttc catcaaattt aaacttttta tcttccataa
    10201 ctataaatac ttttttactt gaatttatta tagccttctt cgtaagacca tcttcaattt
    10261 caaaagttgt aagtgaagag ttgaatacat ctactccaca acttcccata aaagtcttat
    10321 caaacttata tttcgatata aactctatag ttggagtacc gacaaaaccg tctaaactga
    10381 gatttaataa acctccagtg gaaattatat ttaaattatt tggttttgaa gtaactacat
    10441 ttagtatatc cagcatattt gtaacaagtg ttactttttt attactttca gctaacaact
    10501 tcgcaagcaa gatgttaatt gtagagatat ctaaaaatat agtttctcta tcttcaatta
    10561 aattaaatgc tttttctgct attattttct ttgttggaat attaatttct tttctgtctt
    10621 ttgtatcctg attctctagt gtttctctag ctagtacagc tcctccataa attcttctta
    10681 ttgaagaaat cttttctata ttttttaaat cttttcttat acaatcttct gtaacattaa
    10741 atttagaact gagctctttg acagtaactc taccttcttt ttttaggata tttaatatag
    10801 cttggattct ttcttctgca aacattttct acctcttttc gcaatatagt attatagtaa
    10861 atattataag ataaaataca atatatcaac tattttaaca tgaaatacaa aattaattta
    10921 atataaaaag tatgtttttt ctaactatat atttagaatt tagtaaataa aaaaattata
    10981 aaactaagaa ggaaaagtaa aacaataata aaaaataaaa aacaataagc aaaatgttga
    11041 aaataatacc aaatcaatat ataataagaa taaagaaaga ttaaaggggg atattttaat
    11101 gcaactaatc ttagatacag gtaatgtgga ggagattaag gagttatgta cttgtttacc
    11161 aatagatggt gtaactacaa atccaagtat tgtttcaaaa gaaaaaaaga attttaagca
    11221 acttataaat gaaattggtg agattatagg tgaggatatg cctatacatg ctcaagtgtt
    11281 aagtacaaag tatgaagaaa tattagaaga agcattatat ataagtagtt tgagaaaaaa
    11341 tatatatgta aaaatacctg taacacaaga tggtttaaga gctataaaag accttcatag
    11401 aaaaggtgta aaaataactg ctactgcaat attcactgct catcaaggtt ttttagctgc
    11461 aaaggctgga gcaaattata ttgcacctta tgtaaatagg cttgataata tatctggtga
    11521 tggagtagct atggtttcag aacttataaa aataatagat acttataaaa tggatacaaa
    11581 agtacttgca gccagtttta aaaatgcaca acaagttata gaattaatgc aacatggggt
    11641 gcatagtgtt acagtaccat atgatatttg taaatctatg atgaatcatc cattaactga
    11701 ttggagtgta gataagttta tagaggattg ggagaatact tttggaaaag gaagtaaaac
    11761 aaataatatt tagtattatg gtaaaagagt agttttgtcc aattttacaa gaacttatat
    11821 aaaaagatga aaatatgaat tttcttttta ataatttcgt tattatctta agttaattca
    11881 tattttcatc ttttttagat ttctagtcct ataaatttcc accaagggaa tgttatacat
    11941 attattataa agtaggttaa aggaatcaaa gttaaaccgt atttgaaaat aaccttgcta
    12001 ttatacagat tttcaccatg accaaccatt attgtagcat gatgaaatgg taatatgtac
    12061 tgtatgctta ctataatgta gacaaataga cataaagtaa cactattttc ctttagtata
    12121 tgtaaacttc caagtaatgg tattactaca gatgatgtag tgacagcact tccaagacat
    12181 atattcaaga ttattgtgag agttatcaaa aacaatattg aaataatttc tgaagagttt
    12241 ggtataaact ttatcaaata atttcccatt atatctacaa ctccagagta ctttaacact
    12301 cctccaattg aaaatgctgc tataaaaaaa ataaggacct tccaatttat ttcttttaaa
    12361 gtactaaatc caactatttt tcttagtaat aaaagaagta cacaaattga cattataatt
    12421 aatgtgttta tgttatgtat gaactgagtt aaaaacataa aaaacattat tcctaggcat
    12481 aaaaatacgt gtttttgttc tttgtttagc aaattaaatt ctttttttaa attagaatat
    12541 gaaaaatgaa gtttattacc ctcaagattt tcattagaaa taaattttat tgaatgcaat
    12601 tcttttttaa agacaaaggt aaataaaaaa taagttataa aacaagtaat tatagttgga
    12661 acagacatat aaaaagccca ttgaccccaa tttatattac aatttccaag ttctagtaca
    12721 acataattta gaagagtgtc tccatttaca aaaaacattg atgtaaaggt agatgctgta
    12781 aatatactaa ataaaagtat agattttgaa tcttcagtta tctgttgttc ttttaaaaac
    12841 tccttgtaaa aagcagatac aagaataacc cttggaaatg gttgtgggat aaaaaatatt
    12901 aaaagtatac ctaaaatata cgataaaaga atcatttttt taggggtatt tacagtattt
    12961 aaaattaact tctcagaaat aatttttgcg acacctgttt ttgtaactgc attagtaatt
    13021 atgtatgcaa gaattattat gtaaaaattt tctgtaaata aaaactgtaa aataaccttt
    13081 attggtgcaa tgttaaatat aaacgctaga attacaaaaa acaatgcaac aaaagttttt
    13141 tcaactgagt ctacagccca tatcaagata gttaaaaaag ataagcatat agaaaatttt
    13201 atgttgaaac tgtaattaag agagaagtag agaattatgg tagctataac agtcatataa
    13261 aattgttttg gcttaagaat aatacccatt tttatattca cctccataat ttattatatc
    13321 ataatggagg tgaatacaat gaatgtgaat acattttata aaaagattat tcattgattc
    13381 tatgatttac aatagcttta gttttccaat gtcctttctt atagtagata actgtaacaa
    13441 taagacctaa agtccaacca aaaggaatac ccatccaaat tccgtctgtt ccaaaaaatt
    13501 tagaaagcat ataagaagct ggaagtcttc caagccataa agatactatt gagcttataa
    13561 gtgcaaacat ggaatcacca gcacctttca ttgcacttga aagtacaaaa gaagttccaa
    13621 taaataagaa gaatggtcct attataaata agtaagaaga gccaatttta atgacatctt
    13681 tatcagtatt gaatagagca attagatttt ccctaaatag caaaattaaa gcaataactg
    13741 taactgctaa aactaaggtc atttttatag attctcgaat gcctttttta actctttcat
    13801 tttctccagc acctatgttt tgagcaacaa aagtagaaac agcagaacca agattcataa
    13861 taggtaagct tataaaattt tctatcctaa gaccagcacc aaaagctgcc atagcagatg
    13921 taccataact attaactaaa aattgaagag ccatatttcc aattgaaaac aacatttgtt
    13981 gaactccact tggaagtcct aatctaagtc catctttaaa tattttgtta tcatatacaa
    14041 ttcccttgat tttaaattta agcacttcat gttttttatt taaataaatt atacttatta
    14101 taaatgatac tgcctgagcc attatagtgg caagtgcaac gccctcaaca cccattctta
    14161 gcactacaat aaaagttaag tctaaaacta tatttagaat tgtggctatt atcagaaaat
    14221 aaagtggagt tactgaatct ccaagtcctc taagtattgc acttactgaa ttatatccag
    14281 cagaaaataa tattccacta aatattatag taagatacaa ttttgattga gcaaaaacgg
    14341 attctggtgt attcataagc ttaagcattg ggccaccaaa tactattcca agaatactta
    14401 ttaatataga accaataaat aaaagtatat atgtagtatc aatagtagct tttacctttt
    14461 ttaaatctcc tgagccataa aattgtgaca ctaaaataga aaatccaagg ctaacaccca
    14521 taagcgctgc caccaataaa aacattatag ggttagctcc agagactgcc gccattgctt
    14581 cttttccaat aaatctacca actattatac tgtcagctgt attgtatagt tgttgaaata
    14641 aactaccaat aagcataggc atggcaaaaa agaatatgga ctttccctca tgacctgtag
    14701 ttaaatcttt caccttattc ctccaaataa aaaaattatt aatttattat agtataatct
    14761 taacataaac tttatatcca aactataaat aaattgtatc aaaatatcat atattttttt
    14821 aaaatgtaaa gaatataatt ataatttgaa tttaataaat caatatttaa tataatcgaa
    14881 tattaataca aaatcaatac aatatatacg tatatattga caaaattaat tatataaatt
    14941 aaaataatat tcgtaaagaa aaattcatgt tttgtactgt ataacatata attttgacaa
    15001 tatgggtcat aagtttctac cggaataccg taaatattct gactatgtat actattattt
    15061 agttaattaa atatttaatt aactgaatta tgaaggatac ggctcagctt atcaactact
    15121 gtgtcgtttt tttgtaaaat ttaataggag gtgttgttat gcaaacaaca aacagtagta
    15181 ctatgcagaa actttttcca attttgacta ataaaaatgt cgacatgaag aaggaaataa
    15241 ttgcaggagt aactactttt ttaactatgg cttatataat agctgtaaat ccaaatattt
    15301 tatctgagac aggcatgcct gcaggtgcac ttgttacagg tacttgttta gcagcagcat
    15361 ttggatgtat tttaatgggt gtagttgcta accttccttt tgctttagca tcaggtatgg
    15421 gtcttaatgc attttttgca tatacagtag ttttacaaat gggtgttcct tgggaagtag
    15481 ctcttacagc agtatttgta gaaggtatta tttttatagt attatcagta agtggagttc
    15541 gtgaggctgt tgtaaatgcg ataccaaaaa atatgaaatt agctgttaca ggtggtatag
    15601 gaatatttat tgcacttata ggtctagtta acagtggaat tgttattgga gaccaagcca
    15661 ctcttatcaa aatgggtaga tttacaccag cagtaataat aacttgtgta ggattaataa
    15721 ttattgctgt attagataaa aaaagagtta aaggttctat actttttgga atagtagtga
    15781 gttcattact tgcttgggca tttgctttta taaatccaga gtatgcacaa aaattaggta
    15841 tatatttacc tggtggatta tttaaattcg aaagtttagc accaatagca ggtaaaatag
    15901 atttaggata tgtactacat ccaacaaata taggtggttt tttagtagta gtttgtacat
    15961 ttttatttgt tgattttttt gatacagtag gtacattagt tggtgtttgt tctaaagcaa
    16021 acatgttaga tgaaaaaggg aatgttccta atgtaggtag agccttactt acagatgcgg
    16081 ttgcaactac agttggtgca ggacttggag tatctacagt tacaacttat gttgagagtt
    16141 caacaggagt tatcgcaggt ggaagaactg gatggacagc aataacagtt ggaattttat
    16201 tcttagctgc aatgtttttc tctcctgtat ttatagcaat tccatcatgt gctacagcac
    16261 cagctttaat atatgttgga tatttaatgc ttggtacagt taaggatata gagtttgata
    16321 atataactga aggtgttcca gcttttgtta ctattgcttg tatggcatta acatatagta
    16381 taggtgatgg tttaacttta ggtatattaa cttatgtttt cgtaaatata ttttataata
    16441 tatttggagc taaaaaggca gaagacaaaa aacatgtttc tgtagtaatg attgttctgg
    16501 caatattatt tataataaaa ctttggctac tataataggt attatttaaa attttaattt
    16561 aatataaaat aaagggctgt aatttattat caattgtgat gataaactac agctcttttg
    16621 tatttgttgt atttttgtat ttattgtatt atttaataat gtctattagt tctccatttt
    16681 gtaaagtttt aggtgtaatt actatattga actctttttg caaatctttt ataatatcta
    16741 attctttttt tgttattatt gagattgtgt taccacttcg atttcctcta gcagttcgac
    16801 cacatctatg caggtattca tttttacttt ttggaaaatc caagttaaaa acatgactta
    16861 catccacaat atctagtcca cgagcagata aatcagttgt aattaaaatc tttgctttac
    16921 caagtttaaa tttatttatt gcattctttc tatcttcttt ttccatattt ccaaaaattc
    16981 ctattgcttt gtaattatga taatttagtt ttgatactag gacttctata tttttttcat
    17041 tatttacaaa tacaatagct ttttttggat ttgtagctgc taatgccttt ctaagaaaag
    17101 taaatttatc ccttatctca cctaataaat atgagtgatt gatgttagga tttatttgtg
    17161 atttttcctt tgtttttatt atttcaaact ccttcataag tttatcacaa atttttatag
    17221 tgctatcagt aagacttgca gagcatccta taatttgtct atctcttaac gttgttctta
    17281 ttatgtcttc tatacaagtt atattttttc cattcaatag attgtccact tcatctaaga
    17341 caatagtttt tatattatgt gattttagtt tcttttgttt tatcaagtct aaaactcttc
    17401 cacaagagcc aattactatg tgtggtttta ctgcttttat attttttatt tgtttttgta
    17461 tattgacctc cccaattaaa gctaaagaag taacggagag ttctgcattt ttagccaata
    17521 attcaacctg gtttgtaatc tgcattacta attcatgcgt aggtgcaagt ataagagctt
    17581 gtgtttctct ttttgatgta tcaatttttt caaatatagg taaaaggtac gctagtgttt
    17641 ttcctgttcc agtctgtgaa ttaatcaata aatccttatt ttgaattata ttccctataa
    17701 caagtgactg aacttctgtt ggagaagtta tgtcttgttt tttgagtcca tctattagag
    17761 tggaacttat ttttaattgt tcaaatgtat tcatatttta ctccttatat tttaataatt
    17821 atagtcaact attttttatt atacattgtt tattcaaatc gaactatata ttcttttata
    17881 attatgtgta atttgaaagt aaatatcaat tatatttata cctttatatt tctgatacct
    17941 ttgcatttta tagatttcta aaataaacaa ataaaagttg taattgtttg atatttgtta
    18001 tatattgtat ataacaaata tcaaaactat aatatggagg gaaattatgt taaaagtaat
    18061 caatgtatct aaatcatata aaaataaaaa agtagtaagt agtgtctcat ttgatgtaaa
    18121 tgatggagag ctttttgggt ttataggaca taatggagca ggtaaaacta ctactattaa
    18181 agcaattgtt ggaatacacg attttgaaga gggagaagta ctaataaatt ctaaatctat
    18241 aaaaaaacag cctgttgaat gtaaaaagga aatggcatat atacctgata atccagattt
    18301 atacgagtca ttaactggta tacaatacct taattttata gctgacatat ttgaagttgc
    18361 caaagataaa agagaagagt taataaaata ttattcaaat aagtttgaac tcaataaagc
    18421 attaggagat ttaatatctt catattcgca tggtatgaaa caaaaattag ctataatttc
    18481 ggctttgata cacagtccaa aaatcttaat tcttgatgaa ccttttgtag gtcttgaccc
    18541 aaaagcttcc tttatattga aggaaattat gagggagttt tgtgacaaag gtggttgtat
    18601 atttttctca acacacgttt tagaagttgc tgaaaaaatt tgtgataaga ttgccataat
    18661 aaaagatgga aatataattg cttatggaac tacagaagaa gttaagggaa gtaattcact
    18721 tgaaagtatc tttatggaat taatagagaa ataaaatgtt ttttatacaa tatagtgtct
    18781 aaagtgagga atgataaaat atgagcaatc taagtatatt aataaaaact aatataataa
    18841 atgaatttaa attaaatgcg ttaaaaaaag ctgatggtag agaaaaacaa aaaataatgg
    18901 gaatgatgct ttcagttgtt ataatagcgg gtctattaat tttttatgta acaagcttaa
    18961 gtctctcttt agttgatatc ttaaagcaaa taaatcaact ggaaatgcta ctgatatttg
    19021 gttttggatt atcaacatta acaactattg ttacatcatt atataaaacg tcatcatatc
    19081 tatttcaagc aaaagattta gaccttttga ctagtttacc tataaaagaa tctactgtac
    19141 tagcaagtaa gatattaatg ttggttggag caaactattt attttcttca atatgtatgc
    19201 ttatacctgc agtggtttac tttttaaatt ctgatgtaag tataacttat ttcccttatt
    19261 tgatattgct atttttagct ttaccattat taccgatagt tgcttcatca ataatttttc
    19321 tatttatagg gcagatatca tcaagaataa aatacaaaaa tttagtatta atcataggta
    19381 gcattatatt gatgttgctt tataccctac ttatgtcaag acttaattct atgtcagttg
    19441 aaatgctaaa gaatagtgtg tctatttcag agacgattag aaaagtatat cctcctatat
    19501 attattttgt tgatgctctt aaaacaggaa gtatagtttc atttttagca tttatagcca
    19561 tctcaataat accatttttt atgtttgtaa ctctgtttgc aaaaaaattt aaagatataa
    19621 atgctaagat gagtgaagga tataagtcaa aaaattatga atttaagggt caaaaaactt
    19681 caagaccttc aaaagcttta ttaaaaaaag aatttaagag atatacttct tcgtttatat
    19741 actttttaaa ttcatctgca ggtttgttat ttctaatagt aggtacaatt ttaatattat
    19801 tatttggagc agataaagtg ggtgagttac tgaaattgaa tatagatttt aatcttataa
    19861 aaatacaatt attgggagta ttcttcttta taatagtaat gaattgtaca acatactgtt
    19921 caatttctct tgaaggaaaa aacttatgga tactgaaatc ttctccaata gatgaaagag
    19981 aaatattctg gtcaaaaata ttgatgaatt ttttattgaa tgcaccacta tctattttat
    20041 gtctaatatt agtatctata agattagact ttgaaattca gtttattatt gtatcaatct
    20101 gtttgattgt aagtttttca atgtttgtag cattgatggg attattatca aatttattat
    20161 acccaaacct tgattggaaa aatgaggtgg ctgtagtaaa aagaagtgca agtatgatag
    20221 ttacaatgtt agtatcatta gcttatgtaa ttatacttgg aggtatatat gtattacttg
    20281 gaatttcgtt tttaaatata tatattattt tggcaagtat acttactttg atattagatt
    20341 ttatgatatg gagaattatt tctactaaag gagtaagaat attt
//
LOCUS       DAEPIN010000067        20271 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.067, whole genome shotgun sequence.
ACCESSION   DAEPIN010000067 DAEPIN010000000
VERSION     DAEPIN010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20271
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.067"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            265..2352
                     /locus_tag="KR250_002869"
     CDS             265..2352
                     /locus_tag="KR250_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBH0068784.1"
                     /translation="MVVDKEIVSILQILLNSTLITTNGLQEESNSSKRQITYRINKIN
                     DMLKVKNVPLICLRADKDIIVRKETKKAIKEILEKNYSKNTYYFSKTERLLYMYLMLF
                     INMEDISINHFINSIKVSRSTVNLDFKDLIPELEKKNIKVKNNRINGYYIVGNEMEIR
                     RVLIKNIIETLSNEKSSRVFDIFIKEYNLDSFEEAKKIILKLIKKYEITFVEDRLIEF
                     IYIFIFLKARMYSTRIVTQENVDIPNISVMSSMKEYKFAKELLEVYGSEDKIKPHNIM
                     YISAWILGISVGNYEEDTEDKDVISKIVNKMMTKFGYLSGVYYISQEKIFKQLYSHFR
                     PAYYRLLFKLPIYNPICEKVKEEYKLVYRIVSNAMKEFCGLFGEEIQEEELAYLTMHF
                     ATLFSNKKEFDGFRKRIALIVCSNGVGSSAILHAELTNLFPDLHFIPMSESVGLENIS
                     EPVDIVFTTNYNAEGLKTDVPVIKVSPVMTTKEKYKITREVYIQLGDIFLRQPKIDEV
                     MDIIKKYANVTSESILSSELLAYFTQIENFTSKEGEGPMLSEITNETLVKIKVKAKNW
                     EEAIRQSASVLVENNKVTEGYVDAMINSAKASGPYIVITKHVALPHARPEAGSKEIAI
                     GIATLDSPIEFGNNENDPVKYVFCLSAIDNNSHLRAMSELVELLEEDEFFKVLDSAKE
                     AKEIIDFIKTHEL"
     gene            2367..2654
                     /locus_tag="KR250_002870"
     CDS             2367..2654
                     /locus_tag="KR250_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBH0068785.1"
                     /translation="MYKILIACRAGVGSSLMLKIKTQQIIKENNFPIEVEHGSLDSLN
                     GFNGDAVETLIDVAEELKNKNLKFDIIGIHNIVDRNEIKTALEKFLASKEG"
     gene            2681..3952
                     /locus_tag="KR250_002871"
     CDS             2681..3952
                     /locus_tag="KR250_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HBH0068786.1"
                     /translation="MEFIISLLSTPAVLLGVVACIGLILQKKSAVEVFTGSSKTLIGF
                     LIFGIGASAMTGALQNFNKLFQHGFGITGVIASPEAATALAQGTYGFAVSCTLILGFI
                     LNLVFARITKFKNIFFTTGHSLFFSCVLVLIMKAHGFDNTITILIGGTILGFMSAALP
                     QFCQPFMRELTGGDEQAIGHFNMIGYGLSGYIGRLFSKHKDDTTETIEFPKWLSIFRD
                     FLMGLSIVMLILFYIATLKAGQAFTQEIAGSTHWLVFPLIEAFTFVAGMSILMSGVRM
                     FLAEITAAFVIISEKYIPGSRPALDVPTVFPYAPNAVIIGFISAYAAGLLAIAIMASF
                     PSIFPVVIIPAAHICFFSGGTAAIFGNTSGGWRGAIAGSFVVGLLLAFLPVILYPVYG
                     TLGIEGATFPNIDYNVVGSILHNILQFIKPI"
     gene            4010..4609
                     /locus_tag="KR250_002872"
     CDS             4010..4609
                     /locus_tag="KR250_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBH0068787.1"
                     /translation="MENTLSEFLDNISNELSEFIASIDETSINKACELILEAEKNHGR
                     VHVTGIGKPGHVSGYISSLLSSTGTSAYILHGTEAVHGSSGQVVEGDVVIAISNSGET
                     QELKATLKTLKVNGAKIIGVSGNESSFLKNISDIFLFAGVKQEGDCLNKAPRASILAE
                     TIILQSLSVVLQYAKGLNTQQYLKWHPAGSLGKSIREGI"
     gene            4614..5519
                     /gene="dapA"
                     /locus_tag="KR250_002873"
     CDS             4614..5519
                     /gene="dapA"
                     /locus_tag="KR250_002873"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HBH0068788.1"
                     /translation="MKYKGIICAMITPFDENQNINSQATCQLIDYLIEKGIYGLFILG
                     TNGECHVLTDDEKVEFAKIVINHTNNRVPVFVGTGGNSTREVVDLSKRMEAIGASALS
                     IITPYFVTPTQQELILHYKTIANSVNLPIIMYNMPSKTGINIEPESVRELSKIKNIVG
                     IKDSSGKLDNMKAYIEATKNENFSVFSGSDSLILDSLKAGSQGAVAATTNFLTEIGIS
                     IYENFMKGDLEEAQKAQSSIEELRRILKLGTIPSVMKKTIVLNGINVGTARLPVTEPT
                     GEVLEEIKRVVENYRTILNKSSENR"
     gene            5625..6257
                     /locus_tag="KR250_002874"
     CDS             5625..6257
                     /locus_tag="KR250_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketohydroxyglutarate aldolase"
                     /protein_id="HBH0068789.1"
                     /translation="MKKMNITKRMSECGALAIVREENLNRACEIAEGCIKGGITVIEM
                     SYTLNNAGEIIQGLNKKYGETLCVGAGTVLDSETARHAILHGAQFIIAPNYNEGVAKI
                     CNRYQIPYAPGCTSLTEAVDALSLGATFIKAFPISDFYGPKLVKVFKTPIPDMPVLAS
                     GGINIENLHIWLENGVDVCGFGGLLTKGSVEDIAKNAKKIREIIKNIRKI"
     gene            6735..9242
                     /locus_tag="KR250_002875"
     CDS             6735..9242
                     /locus_tag="KR250_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBH0068790.1"
                     /translation="MKEDIRLHRMCKKEQATKNEVISEDILKSMSIGVSKHFLDDSFT
                     IIWGNTSFYQLLGYTEEEFLVKFSSLKDYYEQNLYHFESMKNHFLEAYHHGEKSIEYQ
                     ICISANNGDPVWVIMTGILIESQDNEKSMVYILYSKINELKIKQEELSDMEKESTENF
                     KWMMSEYAGNVYISDMDTYELLYLNKHSCNTLQASANELIGRKCYEVIQGRTSPCPFC
                     TNSYLKKDDIYEWEFYNKKLKRTFIIKDRMLNWQGRRVRIELSYDMYSAEYKLAKKDQ
                     ERESILKTIPAGMVRLDARDYLTILWCNDIFLNMIEYTKEQFAEELNNQCGYLFSEDY
                     KRSKKLAQDLKESGDNVVFEAKIYTRSREERIWTVTLCYISGEDSWDGIPSFYSIGID
                     ITKERKQIEKLQHISEKDALTGIYNRAETERQIKKYFEKNLNVMGALFMIDTDNFKQI
                     NDTEGHMIGDIVLTEMASGMKKIMRDSDVVGRIGGDEFTIFMKNISSVKDAEKKAEEL
                     LYMFRHLFQKEKSFLKVTCSIGIAIYPKDGTTFKEIYARADKALYQAKNMGKNNYVIY
                     NQDYFKELEEFDCSSLGTVIDSEKQYAEYPDNLTRYIFRMLYQTDDIDQAINMILEVV
                     GKQFDVSRVYIFENTEDGRYTSNTYEWCNEGISSQMAYLQNSNYQDYEDYEKLFEDEL
                     VFYCRDIHTLSPKLEELFSSQGIHSTLQCAYKEDRVFSGFVGFDECTGLRLWSQEEIS
                     TLSLISQIISMFLQRKKNNKLNQQIQQYQNILDNIDQCICVINKENNFLLYVNKKFKN
                     TYSKFQIGQPYYLDSIDIHKISIIWNKKEAYLCTSTI"
     gene            9584..11746
                     /locus_tag="KR250_002876"
     CDS             9584..11746
                     /locus_tag="KR250_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HBH0068791.1"
                     /translation="MKKIIYVLFTIELLIVSLLGLNIVKDNEINNILYNETTSISVMF
                     KDYKKLNKNYSKWIKDIADENNVTISKYVFNNNEKLSIYTTDTSLNNNIKLKSGKFPN
                     TNSNEFISNINTNNSNQVGKFSTMSKDISILIRDFGKISEVGESGILYISTQNEDTIN
                     KILRELNVDRSIFVARLHDRYVNSNLYLNPSIIRDLILIILCFLATIIHYSISVSREA
                     SILRLNGYSKLDIIARVIKNILRIMILSSVTALLIYIIYILKLNIGVRACLYFAMFSI
                     ICILINILISILIVGFNSRSSKYVLSLNGKKSYGFVNIMHFTLKIVFVSFLILSINNC
                     ILNYNMLQTQLGNLSEWDKAKNVYNLTLKDTGEQSLGKEEVIKNAKIKGFYKNLVEEK
                     DAFLIDSTNFEKLEDGSYMYEINSKGKNPEVSQYGKNIKINKNYLKVNPIYSNGKEIF
                     NLIDYDDKTINLLVPKKLQVHENEIKKEFRELFYFEKIEVENMYNKELNRALNKTKKS
                     DLNVNIIYVDNNQSYFTYNSLVMDDNRNLIDDPIAIVDIDNIDDSFYLSYISRCVYFN
                     SKKLDALADISSIIEAQGVEAHIQSLHAVYNEYGLEINKLEKYLNSEIFTIIIIAISN
                     LMITYNIVASYYERNKYKLYIKKIFGYSTTARSMLLIVSLILTNIIPIGIISTRVDLS
                     NNIILFGLLILVIEVIVSIVLDKIISNSSFNKIIKGEH"
     gene            11751..12368
                     /locus_tag="KR250_002877"
     CDS             11751..12368
                     /locus_tag="KR250_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0068792.1"
                     /translation="MISLINISKKFKDKSIIDKFNLEISKGEFVAITGNSGSGKTTLL
                     NIMGLLEKPNSGDIIINNIKNPNSKQIRILQRDFYGYLFQNYALIENENVENNLKIAL
                     KYQKNINHNKKINYALESVGLRKYNKKKIYELSGGEQQRVALARVILKECEVIFADEP
                     TGNLDKKNRDIVFDILKSLNKNGKTIVFVTHDLELSEQADRVIKL"
     gene            12557..12901
                     /locus_tag="KR250_002878"
     CDS             12557..12901
                     /locus_tag="KR250_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HBH0068793.1"
                     /translation="MENLPNCPKCNSEYTYEDGTLLVCPECSHEWALGLGAEEDSNII
                     KDVNGNVLNDGDSVTVIRDLKVKGSSSSIKIGTKVKNIRLIHDSSDGHDIECKIDGFG
                     AMKLKSSVVKKA"
     gene            13471..14337
                     /locus_tag="KR250_002879"
     CDS             13471..14337
                     /locus_tag="KR250_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBH0068794.1"
                     /translation="MIDIHKNIYDNKLFEELKIDCKKCFGLCCVALYFSASDGFPIDK
                     ESGKPCINLQPDFKCSVHNSLMKRGFKGCTAYDCFGSGQKVAQVTYKGIDWMQSSELT
                     NQMSEVFLIMRQLHEMLWYLKEASVLNISDTIKSKIDLIIEETEKITNMSPEQIINLD
                     IISHRIKVNLLLSQASESVMGKVKSFIKTSTLKNMKKLSKNIDLIGADLRKINLIGAD
                     LRGRFLIAANLRNTDLSGANLIGADLRDCDIRGANLENSIFLTQLQVNTAKGDSSTKL
                     PASLIRPKYWEK"
     gene            14450..14854
                     /locus_tag="KR250_002880"
     CDS             14450..14854
                     /locus_tag="KR250_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmaF family protein"
                     /protein_id="HBH0068795.1"
                     /translation="MSYQNYKCERDYVYDDYKYNKCNKCYNDYEEMTHVHEYSESVKL
                     AEECEDRHNHRAAGVTGEAIPINGGTNHVHKINDNVDFLDHFHKICVTTGPAIRIPGT
                     DKHIHLICGETTVNDGHCHKFLFTTQIEAPLV"
     gene            15021..15470
                     /locus_tag="KR250_002881"
     CDS             15021..15470
                     /locus_tag="KR250_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBH0068796.1"
                     /translation="MKSGIILEMKQINSTEYDEMLQVWESSVRATHDFLTEKDIESLI
                     PLVEIGLKEVENIVCIKDNDIIKGFIGIDKDKIEMLFIEDKYRGNGIGKKLIKYAIDK
                     YNVKYVDVNEQNKKAVGFYIHLGFKVFDRSEIDGQGNPFPILHMKLI"
     gene            complement(15626..16003)
                     /locus_tag="KR250_002882"
     CDS             complement(15626..16003)
                     /locus_tag="KR250_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBH0068797.1"
                     /translation="MLTPYLIFNGTCEKAFNFYAEAFGGGKTIFARLDSNPNNPIMHA
                     SVTFTKYEGCIMGADTDKPVVISGMAICVVLPSREAIEEISVKLAEGGTLVQEFLPHP
                     PPHQNDGAAEVLDRYGYTWYLST"
     gene            16265..17182
                     /locus_tag="KR250_002883"
     CDS             16265..17182
                     /locus_tag="KR250_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2268 domain-containing protein"
                     /protein_id="HBH0068798.1"
                     /translation="MVNMNIIEIRSDKIYKKIMDAPINKKEDIYRYELMKPFEFKWKC
                     MNVPIVARQKGGYDVIIASEMLGVLSPKDIDEKQKKNINVLSADKIWATCKETIENSI
                     NAFIKEGYDLNIKDYKYSILLANPNSPYTILSDGYWGDGGIPGYIFLSLVPNEYTINR
                     LPVLIAHECNHNIRFQFIEWNNNITLEEMMINEGLAENFATWMFGEEMLGPWVSRTDI
                     ETLNTYIKPIIKSALKETGFQNITSYLYGDDIAKMQGYFPVGLPYCAGYACGYYMIKY
                     YLEKTNKSIIEATLLPYSEIIEAVKEFWE"
     gene            17666..19084
                     /locus_tag="KR250_002884"
     CDS             17666..19084
                     /locus_tag="KR250_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HBH0068799.1"
                     /translation="MTELMIISGLVLLICITSSKVLYKFGVPILLIFILLGMLFGSDG
                     MVGIYFDNYELTKQLCSIGLIFIMFFGGFGTNWNMAKPVAIPSILMSSLGVIITAGVT
                     GMFCHMVIGTSLLEGLLIGAIAASTDAASVFAVLRSQKLNLKGSLASLLEVESGSNDP
                     IAYMLTLILLTLMNNSGVELIAPMVIKQIAFGLAIGFILAKLSVYILRHSNFEIEGFY
                     TIFITAIAILSYAFSEYLGGNGYLSVYISGIIIGNSRIPLKKSLVHFFDGISWIMQIM
                     LFFMLGLLSFPSKLPNVFGIAMAISIFMIVIARPLATFIVLAKFKFSNKEKLFISWVG
                     LRGAASVVFAIYAVTQGVAIENDIFHIIFFIALLSVGIQGSLIPTVARKLDLVDDNTP
                     VLKTFNDYDGEISSKLIEVNVDEGNKWANKSIIDSNIPDDILIVMIKRKEQVLIPKGS
                     TIIKTGDILVLSGDRIEELIQL"
     gene            19160..19972
                     /locus_tag="KR250_002885"
     CDS             19160..19972
                     /locus_tag="KR250_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBH0068800.1"
                     /translation="MFSIGMFSKINKITTKTLRYYEDIGLLKPEYVDEFTKYRYYTTE
                     QLPKLHQIITLKQMGLSLAEIKKAVENPEEVENILMNKEKEMLDVIKSEEYKIIKLRN
                     YLMNLKGEVNMNDVIIKSLPKVKVASMRKVLKGYDELFNLFPNVMAEEMMKTGCTCAV
                     PDYCFNIYHDGEYRETDMDVEMCQAINDLKEDTDILKFKELDAVENAVCILHNGPYST
                     LGKTYADAFKWIEDNKYKVVGNPRESYIDGIWNKESEEDWLTEVQIPVKRML"
BASE COUNT         7906 a         2165 c         3422 g         6778 t
ORIGIN      
        1 atttataatc aatatgattt ttattattta aatcaatttt tcttgagttt ctttgtctaa
       61 agaaactttt tttatttata aaatctacag ttatattata gtatctattt tagatgagtt
      121 taatttttgt atcaatctaa aacaaaagtt atacttaaat gaaattgaaa acgataaaat
      181 tctcaaataa aataagtatt gtggaggtga tgaaagaatc caatgataat atgaagacta
      241 atcttaataa gaaagtaggg gttaatggta gtggacaaag aaattgtatc aattctccag
      301 attttattaa atagcacatt gattacaact aatggattac aagaggagtc taattcatca
      361 aagagacaaa ttacttatag aataaataaa atcaatgaca tgcttaaagt aaaaaatgta
      421 ccacttatat gtttaagagc cgacaaagat attatcgtta gaaaagaaac aaaaaaagca
      481 attaaagaaa ttttagaaaa aaactattct aagaatactt attattttag taagacagaa
      541 agacttttat atatgtattt aatgcttttt attaacatgg aggatatttc aattaatcat
      601 tttataaatt ctataaaagt aagtaggtct actgtaaatc ttgatttcaa ggatttgata
      661 ccagagcttg aaaagaagaa cattaaagtt aagaacaatc gtataaatgg atattacata
      721 gttggcaatg agatggaaat taggagagta cttattaaaa acattataga aactttgtct
      781 aatgagaaaa gttcaagagt atttgatatt tttattaaag aatataattt agatagtttt
      841 gaagaggcta aaaaaattat attaaagcta ataaaaaaat atgaaattac ttttgttgaa
      901 gatagattaa tagaatttat atatatattt atctttctaa aagcaaggat gtattcaact
      961 agaatagtta ctcaggaaaa tgtagatatt cctaatattt cagttatgag ttcaatgaag
     1021 gagtataaat ttgctaagga attattagaa gtatatggga gtgaagataa gataaagcct
     1081 cacaatataa tgtatattag tgcttggatt ttaggtatat cagtaggaaa ttatgaagaa
     1141 gatacagaag ataaggatgt aatttcaaaa attgtaaata aaatgatgac aaagtttgga
     1201 tatttaagcg gagtttacta tataagccaa gaaaaaatat ttaaacaatt atattctcat
     1261 tttagacctg cctactatag acttttattt aaattaccta tctataatcc gatttgtgaa
     1321 aaagtaaaag aagaatataa attagtttat agaattgtaa gtaatgcaat gaaagagttt
     1381 tgcggattat ttggagaaga aattcaggaa gaagagttag catatcttac aatgcacttt
     1441 gcaacacttt tttcaaataa aaaagaattt gatggtttta gaaaaagaat agcattaata
     1501 gtttgttcaa atggcgttgg tagttcagct attttacatg cagaacttac aaacttattt
     1561 cctgacttac actttatacc aatgtcggaa tcagttggat tagaaaatat aagtgaacct
     1621 gtagatattg tatttactac taattataac gcagagggtt taaaaacaga tgtaccagta
     1681 attaaagtaa gccctgttat gaccacaaaa gaaaaatata agattacacg tgaagtgtat
     1741 attcaattag gtgatatttt tttaagacaa cctaaaatag atgaggttat ggatataata
     1801 aaaaaatatg caaatgtaac ctctgaaagt attctttcaa gtgagctatt agcttatttt
     1861 acacagatag agaattttac atctaaggaa ggagaggggc ctatgctaag tgagattact
     1921 aatgaaacac tagtaaaaat aaaggtaaaa gctaagaatt gggaggaagc tattagacag
     1981 tcagcttctg tattagttga aaataacaaa gtaactgaag ggtatgtaga tgctatgatt
     2041 aactcagcaa aagcatctgg accatacata gtaattacaa aacatgttgc acttccacat
     2101 gctagaccag aagctgggtc aaaagagatt gcaataggca ttgctacttt agatagtcct
     2161 attgagtttg ggaataacga aaacgaccct gtaaaatatg tattttgtct aagtgcaata
     2221 gacaataata gccatttacg tgctatgtct gagctagttg aactattgga agaagatgag
     2281 ttttttaagg ttttagatag tgctaaagaa gctaaagaaa ttattgattt tataaaaaca
     2341 cacgaattat aaaggaggat ttaattatgt ataaaatttt aatcgcatgt agagcaggag
     2401 ttggctcaag tttaatgtta aaaataaaga ctcaacaaat tattaaggaa aataactttc
     2461 caattgaagt agagcatggt tcattggatt cactaaatgg attcaatgga gatgctgtag
     2521 aaacattaat tgatgttgct gaagaattaa aaaataaaaa tttgaagttt gatataattg
     2581 ggattcacaa tattgttgac cgtaatgaaa taaagacagc actagaaaaa ttccttgctt
     2641 caaaagaggg ataaatttaa gatgataagg aggaatattt atggagttta ttattagttt
     2701 gctgagtaca cctgctgttt tgcttggcgt agttgcatgt ataggattga ttcttcaaaa
     2761 gaaatcagct gttgaagtat ttacaggctc ttcaaaaaca cttatcggtt ttttgatatt
     2821 tggtattggt gcctctgcta tgacaggagc acttcaaaat ttcaataaac tgttccaaca
     2881 tggctttgga attactggtg ttatagcttc accagaagca gctacagcac ttgcacaagg
     2941 tacatatggt tttgcagttt catgtacatt aattcttgga ttcatattaa atttagtatt
     3001 tgcacgtatt acaaagttta agaatatttt ctttacaaca ggtcatagtt tgttcttttc
     3061 ttgtgtatta gttttaatta tgaaagcaca tggatttgat aatacaatca ctattttaat
     3121 aggtggaact atcttagggt ttatgtctgc tgcactacct caattctgtc aaccatttat
     3181 gcgtgaatta acaggtggcg acgaacaagc tattggtcac tttaacatga ttggttatgg
     3241 tttatcaggt tatattgggc gtttattttc aaaacataaa gatgatacaa cagagactat
     3301 agaatttcct aagtggttat caatattcag agacttctta atgggtctat ccatagttat
     3361 gttaatctta ttttatattg caacattaaa agctggtcaa gcatttactc aagaaattgc
     3421 tggttcaaca cattggttgg tttttccgct tatagaggca tttacatttg tggcaggtat
     3481 gtcaatattg atgtcaggtg ttcgtatgtt cttagctgaa ataactgcag catttgtaat
     3541 catttcagaa aagtatattc caggttcacg tcctgcgcta gatgttccaa cagtatttcc
     3601 atatgcacca aatgcagtta ttattgggtt tatatcagca tatgctgctg gtctattagc
     3661 aattgctatt atggcatcat ttcctagtat atttcctgta gtaattattc cagctgctca
     3721 catatgtttc ttctcaggag gtacagctgc aatatttggc aatacttcag gaggatggag
     3781 aggggctatt gctgggtcat ttgttgtggg gttattactt gcatttttac cagttatact
     3841 atatccagtt tatggcactc ttggtataga aggagcaact ttccctaata ttgattacaa
     3901 tgtagttggt tcgatattac ataatatttt acaatttata aaacctattt aaactaaatt
     3961 atataaaagt atagtcatag ttactatgat ttcaaggagg atacatctta tggaaaatac
     4021 attatctgaa tttttagata atatatctaa tgaattgtca gaatttattg cctcaataga
     4081 tgagacatct ataaataaag catgcgaatt aattcttgaa gctgaaaaaa atcacggacg
     4141 tgtacatgtg actggtattg gcaaaccagg acatgtatcg ggatatattt catcattgct
     4201 ttcatctact ggaacgtcag catatatatt gcatggtaca gaagcagtac atgggtcaag
     4261 tggtcaagta gttgaaggag atgttgttat tgcaatatca aatagtggtg aaactcaaga
     4321 gttaaaagca actcttaaga ctctaaaagt aaatggagca aagataattg gtgtaagtgg
     4381 taatgagagt tcatttttaa aaaatatttc tgatatattc ctgtttgcag gtgttaaaca
     4441 ggaaggagat tgtcttaata aagctcctcg tgcttcaatt ttagctgaaa caataatatt
     4501 acaatcatta agtgttgtat tacaatatgc taaaggtctt aatacacagc agtatttgaa
     4561 gtggcatcca gcaggaagtc ttggaaagtc tattagagag ggaatataat tttatgaaat
     4621 ataaagggat tatatgtgca atgataacac catttgatga aaatcaaaat attaattcac
     4681 aagcaacatg tcaattaatt gattatttga ttgaaaaggg aatttatgga ttatttattt
     4741 tgggcactaa tggtgaatgt catgtattaa cagatgatga aaaagttgaa tttgcaaaga
     4801 ttgttattaa tcatacaaat aatcgtgtac ctgtttttgt tggaacaggg ggtaattcaa
     4861 ctcgtgaagt tgtagattta tctaagagaa tggaagcgat tggagcaagt gcattatcta
     4921 ttattacacc atactttgtt acaccaacac aacaggaatt aatcttacat tacaaaacta
     4981 ttgctaattc agttaattta cctattataa tgtataatat gccaagtaag actggaatta
     5041 atattgaacc agagtcagta cgtgaattat caaagataaa gaatattgtt ggaattaagg
     5101 atagtagtgg taaacttgac aacatgaaag cttatattga agcaacgaag aatgagaatt
     5161 ttagtgtgtt ttcaggctca gattcactta ttctggatag tctaaaagct ggtagtcaag
     5221 gagctgttgc agcaacaaca aacttcctca cagagattgg tatctctatc tatgaaaact
     5281 ttatgaaagg ggatttggaa gaagctcaaa aagctcaaag tagtatagaa gaacttcgac
     5341 gtatactgaa acttggaaca attccatctg ttatgaagaa aactattgtc ttgaatggaa
     5401 ttaatgttgg aactgcacga cttcctgtaa ctgaaccaac tggggaagta cttgaagaaa
     5461 ttaaacgagt agttgaaaat tataggacaa tacttaataa aagtagtgag aatagataat
     5521 ataaataggg aaattacact ggtatataat taatatatat attataaaac atgactaaaa
     5581 ttaatgaata agtaaagtgg tttgatgagt atagaggtga taatatgaaa aagatgaata
     5641 taaccaaaag aatgtcagaa tgtggggctc ttgcaattgt aagagaagaa aatcttaacc
     5701 gtgcatgtga aattgctgaa ggatgtataa aaggtggtat aacagttata gaaatgagtt
     5761 ataccctaaa taatgctgga gaaataattc aagggcttaa taaaaagtat ggtgaaacac
     5821 tttgtgtggg agcaggaaca gttttggata gtgaaacagc tcgtcatgct atattacatg
     5881 gagctcaatt tattattgca ccaaattaca atgaaggtgt agcgaagatt tgcaatagat
     5941 atcagattcc ttatgctcca ggatgtacat cattgactga ggcagtagac gcactaagtt
     6001 taggagcaac ttttataaaa gctttcccaa tatcagattt ctatggacct aaactagtaa
     6061 aggtatttaa gacaccaata cctgatatgc cagttttagc aagtggaggg attaatattg
     6121 aaaacctaca tatatggtta gaaaatggag ttgatgtatg tggatttggt ggtttattaa
     6181 ctaaaggttc tgttgaagat attgctaaaa atgctaaaaa gattcgtgaa atcattaaaa
     6241 atataagaaa aatttaacat agggctgtat catagtatct tataattact atgatacagc
     6301 ctttttaata ttattcaaat taaaaacagt ttacatttct attatattac atataatttc
     6361 agtaatttta tcttaatttt tctatctgga aaaaaatgat ttaaaactta agttatgata
     6421 taatttcatt aaaagaaggc gatttttagt aaatatgaga aaataatgtg tttttagggg
     6481 aaaagttaaa tacagattta agtgaacaag aatcgataat taagatatta aatggtgaat
     6541 agggcatcaa aaaaattact aaattaaaag ttttagaggg agaatcttag caagtgtgtg
     6601 tgaaaattca atttcaaaca aaaaataatt gtaaatgaaa tcaatttata cacgttgaag
     6661 attcactttt atcattatat taaaaagcag agtgaattaa aagtgagtct aaagatagga
     6721 ggataattaa aacaatgaaa gaagacatta ggttacatcg aatgtgtaaa aaagaacaag
     6781 caacaaagaa tgaagttata tcagaggata ttctaaaatc tatgagtatt ggggtcagta
     6841 aacatttttt agatgattca tttacaataa tatggggaaa tacatcattc tatcaattat
     6901 taggatatac agaagaagaa tttttagtta aattttctag tttaaaagat tattatgaac
     6961 agaatcttta tcattttgaa tctatgaaaa atcatttttt agaggcttat catcatggcg
     7021 aaaagagtat agaatatcaa atttgcattt cagcaaataa tggagaccct gtttgggtaa
     7081 taatgactgg tatattaatt gaatcacaag ataatgagaa atcaatggtc tatatattgt
     7141 attcaaaaat taatgagtta aaaataaagc aagaagaact atctgatatg gaaaaagaga
     7201 gtacagagaa ttttaaatgg atgatgtcag agtatgctgg gaatgtttat attagcgata
     7261 tggatacata tgaattacta tacttaaata agcactcatg caatacttta caggcatcag
     7321 caaatgaact tattggaaga aaatgttatg aggtaattca aggtagaact tcgccatgtc
     7381 cgttttgtac aaattcgtat ttaaaaaaag atgacatata tgaatgggaa ttttataata
     7441 aaaaactgaa acgaacattt ataattaaag accgtatgtt gaattggcaa gggcgaagag
     7501 ttcgtattga attatcttat gatatgtata gtgctgaata taaattagca aaaaaagatc
     7561 aggaaagaga atcaattttg aaaactattc cagcaggaat ggttagatta gatgcaagag
     7621 attatcttac tatactttgg tgtaatgata tatttttgaa tatgattgaa tatacaaagg
     7681 aacagtttgc agaggaactt aataatcaat gtggatattt attttctgaa gattataaac
     7741 gtagtaagaa actggcacaa gatttgaagg agtctgggga taatgttgta tttgaagcaa
     7801 aaatatatac acgttcaagg gaagaacgta tatggactgt tacattgtgt tatatcagtg
     7861 gggaagacag ttgggatgga ataccatctt tttatagtat aggtattgac ataacaaagg
     7921 aaagaaaaca gattgagaaa ttacagcata tatcagagaa agatgcatta acaggaattt
     7981 ataatcgtgc tgagactgaa aggcagatta agaaatattt tgaaaaaaat ttaaatgtta
     8041 tgggtgcact gtttatgatt gatacagata attttaaaca gattaatgat acagaagggc
     8101 atatgattgg agatatagta cttactgaga tggcatcagg tatgaaaaaa ataatgcgtg
     8161 acagtgatgt tgttggaaga ataggtggag atgagtttac aatttttatg aagaatattt
     8221 cctcagttaa agatgcagaa aagaaagcag aagaactatt gtatatgttc cgtcatctat
     8281 ttcagaaaga aaaaagtttt ctaaaagtaa cttgtagcat aggaatagct atatatccaa
     8341 aggatggtac tacattcaag gaaatatatg caagagctga taaagccctg tatcaagcaa
     8401 aaaatatggg aaaaaataat tatgtaatct acaaccaaga ttattttaaa gaattagaag
     8461 aatttgattg ttcttctctt ggtacagtta ttgattcaga aaaacaatac gctgaatatc
     8521 ctgataatct tacacgttat atattccgta tgctttatca gactgatgat atagaccagg
     8581 cgattaatat gatacttgag gttgttggga aacagtttga tgtaagtcgt gtttatattt
     8641 ttgaaaatac agaagatggt agatatactt ctaatactta tgaatggtgt aatgaaggaa
     8701 tctcttctca aatggcttat ttgcaaaaca gcaattatca agactatgag gattatgaaa
     8761 agctttttga ggatgaatta gttttttatt gtcgagatat ccatacatta agtcctaaac
     8821 tagaagaatt gttttctagt caaggtatcc actctacact tcaatgtgca tataaagaag
     8881 atagagtttt cagtggattt gttggatttg atgaatgcac aggactacgt ctatggtcac
     8941 aagaggaaat aagtacatta tcattgattt ctcaaattat atcaatgttt ttacaacgta
     9001 aaaaaaataa taaacttaat caacagattc aacaatacca gaatatttta gataatattg
     9061 accagtgtat ttgtgtgatt aataaagaaa ataatttttt attatatgta aataaaaaat
     9121 ttaaaaatac atattctaaa ttccaaattg gacaacctta ttatcttgat tctatagata
     9181 ttcataagat atccattata tggaataaaa aagaagcata cctatgtact agcacgattt
     9241 aatggtaaag taaagtttat attggactta aattaaattt cgttttaaat aagatattat
     9301 tttagttgct tactgaaaag actagaatct atattaatga attatactaa ttttatacca
     9361 ctattaaaat attgtttgct aaaaataaaa aatagcctta aaacagttga ttataaaaaa
     9421 ttaactgctt taaggctatt tttatctttg tgacgaaata gacacttgcc atttagaatg
     9481 tgacaatata gtagttgttt agaaaaggca caaagcatta tactttaact aaagtaaaat
     9541 gtattttgct tttagaataa aaacaattag gagggagcta taattgaaaa agataatata
     9601 tgtattattt acaattgaac tattaatagt atctttatta ggtctaaata tagttaaaga
     9661 taatgaaatt aataatattt tatataacga gacaacaagt atatctgtaa tgtttaaaga
     9721 ttataaaaag ttaaataaaa attatagtaa gtggattaaa gatattgcag atgaaaataa
     9781 tgtgactata tcaaagtatg tttttaataa taatgaaaaa ttatctatat atacgactga
     9841 tacaagtttg aataacaata taaagttaaa atcaggaaaa tttcctaata ctaattcaaa
     9901 tgaatttatt agtaatataa acactaataa tagtaatcaa gtaggtaaat tctcaactat
     9961 gagtaaagac atatcaattt taattaggga ttttggtaaa ataagtgaag ttggagaatc
    10021 aggaatctta tatatttcaa ctcaaaatga agatacaata aataaaatat taagagaact
    10081 aaatgtagac agaagtattt ttgtagcaag attacatgat agatatgtta actcaaattt
    10141 atacttaaat ccttcaataa ttagagattt aatactaatt atcctttgtt tcttggctac
    10201 aataatacat tattcaatta gtgtgagtag agaggcttct attttaagat taaatggata
    10261 ctctaagcta gatataattg ctagagttat aaaaaatata cttagaatta tgattttatc
    10321 atctgtcaca gcattattaa tatatataat ctatatttta aaattaaata taggtgttag
    10381 agcatgctta tactttgcta tgttttcgat tatatgtatt ttgataaata ttttgatatc
    10441 tatattaata gttggtttta atagtagaag ttctaaatat gtgctatctc taaatggaaa
    10501 aaaatcttat ggttttgtaa atataatgca tttcacattg aaaattgtat ttgtatcatt
    10561 tcttatatta tccataaata actgtatttt aaattataat atgctacaaa ctcagttagg
    10621 aaatctatct gagtgggata aagcaaaaaa tgtttataat cttactctta aagatacagg
    10681 cgaacaatct ttaggaaagg aagaagtaat taaaaatgcc aagattaaag gtttctataa
    10741 aaatttggta gaagaaaaag atgctttctt aatagattca actaattttg aaaaacttga
    10801 agatggaagt tacatgtatg aaataaattc aaagggtaaa aacccagaag taagtcaata
    10861 tggtaaaaat ataaaaataa ataaaaacta tcttaaagtt aaccctattt actcaaatgg
    10921 caaagaaata tttaatctaa tcgattatga cgataagact ataaatttat tagttcctaa
    10981 aaagttacaa gtacatgaaa atgagattaa aaaagagttt agagaattat tttattttga
    11041 aaaaatagaa gtagaaaata tgtacaataa agagttaaat agagcgttaa acaaaacaaa
    11101 gaagtcagat ttaaatgtta atataatata tgtagataat aatcaaagct attttactta
    11161 taactccctt gttatggatg acaataggaa tctcattgat gacccaatag caatagttga
    11221 catagataat attgatgatt ctttttatct ttcttatata tctagatgtg tatattttaa
    11281 ttctaagaaa ttagatgccc ttgcagatat aagtagcatt attgaagctc aaggtgtaga
    11341 agcgcatatc caaagtttac atgctgttta taatgagtat ggtttagaaa taaataaact
    11401 agaaaaatat ttgaatagtg aaatttttac tataataata atagcaatat caaacttaat
    11461 gattacctac aatatagtag ctagctacta tgaaagaaat aaatataagt tatatattaa
    11521 aaaaatattt ggttattcaa ctacagctag aagtatgttg ttaatagtct cattaatatt
    11581 aacaaacatt attccaatag gcattatatc aacaagagta gatttaagta ataatattat
    11641 cttatttgga cttctaatat tagttataga agtaattgta tctattgtat tagacaagat
    11701 tatctcaaat agttcattta ataaaattat aaaaggagaa cattaaaaag atgatttcat
    11761 taataaatat atctaaaaaa tttaaagata aatctataat agacaaattt aacctagaaa
    11821 tttctaaggg cgagtttgta gcgataacag gaaatagtgg ttcaggtaag acaactttac
    11881 ttaatataat gggattatta gaaaaaccta attctggtga tattataata aataatataa
    11941 aaaatccaaa tagtaaacag ataagaattt tacaaaggga tttttatggc tatctatttc
    12001 aaaattatgc tttaattgag aatgaaaatg ttgaaaataa cttgaaaatt gctttaaagt
    12061 atcaaaaaaa tatcaaccat aataaaaaaa taaattacgc acttgagtct gtaggtctta
    12121 gaaaatacaa taaaaaaaag atatacgaat tgagtggagg agaacaacaa agggtagcat
    12181 tagcaagagt aatattaaaa gaatgtgaag taatatttgc agatgaacct acaggtaatt
    12241 tagataaaaa aaatagagac atagtatttg atatacttaa aagtctaaat aagaatggta
    12301 aaactatcgt atttgtaact catgatttag agttatcgga gcaggcagat agagttataa
    12361 aattgtaata atatcaacta taataaatat aagaaagaga ttacagctaa atcaagttta
    12421 ataataatac gaatagaatt taaacattaa gtaaattagt agtttgtaaa tataagtata
    12481 atatgatatc ataaaggctc aaaagaaatt ttatataata agaaatctaa aaataacaaa
    12541 tggaggacat ataaagatgg aaaatttacc aaattgcccg aaatgtaatt cagaatatac
    12601 ttatgaagat ggaactcttt tagtttgtcc agaatgctct catgagtggg cattaggatt
    12661 gggagctgaa gaagattcaa atattatcaa agatgtaaat ggaaatgtat taaatgatgg
    12721 agattctgtt acagtaatta gagaccttaa agtaaaagga agttcatcat ctataaagat
    12781 tggaacaaaa gtgaaaaata tccgtttaat ccatgactca tcagatggac atgatataga
    12841 atgtaagata gatggttttg gagctatgaa gctaaagtct tctgttgtta aaaaagcgta
    12901 atattataaa taagctgtag tgtttattgt ttttcagtac agtataaaac cataagaatc
    12961 tattaagtaa taaaagtttg tttatttatc tttatatttt cttatggttt tattaaatat
    13021 aaattttatt gaacaccaaa atattctctc ctctacaaat aagccaatct tatttaaatt
    13081 aatatacttt gtactattta tactgaaatt gtaaatttaa atgctatgtt atttaggttt
    13141 gtatagttaa tttatatatt tctgatttta taaattgtat ttattattat atcttgattc
    13201 taaaatcatt aaaaatttca acttctataa agatttacaa tttatcaaat acattatgag
    13261 tatataaaca atttattatt acagtgctac atttaaaata agtcttagta taaaggtatt
    13321 gttacaagta taaagaaact tatgtatgct attggaataa ataatacctt aaattataat
    13381 aaagaataag aactgttaaa taagggactt gattatcatt tagttataga taatataata
    13441 ttaaaaataa agtttaagag gagaaaaaat atgatagata tacataaaaa tatatatgat
    13501 aataagttat ttgaggagct aaagattgac tgtaagaagt gctttggact ttgctgtgta
    13561 gctctttatt tttctgcttc agatggattt cctatcgata aggaatcagg aaaaccctgt
    13621 attaatttac aaccagattt taaatgttct gtacataata gtcttatgaa gcgaggtttt
    13681 aaagggtgta ctgcatatga ttgttttggt tcaggtcaaa aagtagcaca agtaacatat
    13741 aaggggatag actggatgca atcatcagaa ctaacaaatc aaatgtctga agtgttttta
    13801 attatgagac aattacatga aatgttatgg tatcttaaag aagcatctgt attaaatata
    13861 tcagatacaa tcaaatcaaa aattgattta ataattgaag aaactgaaaa gataactaat
    13921 atgagcccag aacagataat taatttggat atcatatcac atagaataaa ggttaattta
    13981 ttattatcac aagcgagtga gtctgtgatg ggaaaggtta aatcatttat caagacttct
    14041 actcttaaaa acatgaaaaa attaagtaaa aatatagatt taataggagc agaccttaga
    14101 aaaataaatc taataggagc agatttaaga ggtagatttc taatagcagc aaatttaaga
    14161 aatacagatt tgagtggggc aaatttaata ggagcagact taagagattg tgatattaga
    14221 ggtgctaatc ttgaaaatag tatattttta acacaattac aagtaaatac tgcaaaagga
    14281 gattctagta cgaagttacc agcttcacta atacgcccta aatactggga aaaataatta
    14341 aattaataca taattttaca aaacatgaac ttttttgact ttaattcata ttatacatta
    14401 gagattcttt acaagaattt cttaatataa tatagggaga gatataaaaa tgagttatca
    14461 aaattataaa tgtgaaagag attatgtgta tgatgattat aaatacaata agtgcaacaa
    14521 gtgctataat gattatgagg aaatgacaca tgttcatgaa tattcagaaa gtgttaagtt
    14581 agcagaagaa tgtgaggata gacataacca ccgtgcagca ggggttactg gtgaagctat
    14641 accaataaat ggaggtacaa atcatgttca taaaataaat gataatgtag atttccttga
    14701 ccatttccat aagatatgtg ttacaacagg gccagcaatt agaatacctg gaactgataa
    14761 acacattcac ttaatatgtg gagaaactac tgttaatgat ggtcattgtc ataaattcct
    14821 ttttactact caaatagaag cacctttagt ataagctgat gtgaattatt gtagtgaatt
    14881 tgctagatta tagtataata atatataata gataatattt aaacttaatg aggactatga
    14941 tatttaacct tcgtagtcct ttctaagttt tgtataaaaa agtgttatgc aattaaaagt
    15001 gatataggga ggaaattttt atgaaatctg gaataattct tgaaatgaaa caaataaatt
    15061 caacagaata tgacgaaatg ttgcaagtct gggaatcatc agtaagagca acgcatgatt
    15121 ttttaacaga aaaagatatt gaatctttga taccattagt tgagattgga cttaaagaag
    15181 tagaaaatat agtttgtata aaagataatg atataataaa gggctttata ggaatagata
    15241 aagataaaat tgagatgctc tttatagaag ataaatatag aggaaatggt attggtaaaa
    15301 aattaataaa atatgctata gataaataca atgtaaaata tgttgatgta aatgagcaga
    15361 ataaaaaagc tgtaggattt tatattcact taggatttaa agtgtttgat aggtctgaaa
    15421 tagatggaca aggtaaccca tttccaatac tacatatgaa attaatttaa agttatactt
    15481 ctcatttatt cgcaaaaata tttacttaaa tttttataca aataaaagtc gtgacagtct
    15541 taggacacga cttttttact acactattaa attgaaattt atctgttcta tattatgatt
    15601 attagatgtc gtttttttat tttaactatg tacttaaata ccaagtatac ccatacctat
    15661 caagtacttc agcagcgcca tcattttgat gtggtggtgg gtgtggtaaa aattcttgta
    15721 caagtgtacc accttcggca agttttacag atatttcttc tatcgcttct cgagatggta
    15781 gaacaacaca aatcgccatg ccagaaatta caacaggctt gtctgtatcc gcacccatta
    15841 tacaaccttc gtattttgtg aaagtaacac ttgcgtgcat aataggattg tttggattgc
    15901 tgtctaatcg cgcaaatata gtttttcctc ctccgaaagc ctcagcataa aaattaaatg
    15961 ctttttcaca agtaccatta aatattaaat atggagttag catgaccaat atatcctcct
    16021 tgatatagaa taatttatag ttgcacctga taatctaaat tatgatttag taaattgtat
    16081 catagggaaa ccttgaagta tataattttt tattattata ttatacaaat ttattaattt
    16141 aaaaaaatgt tatatgtacg gaaggtttac aggataaaat ataataaaaa gattaaggtg
    16201 acattataaa aaatgtatat tgacattgac gctacgtaat tgtatatact ttgtcttaaa
    16261 ggagatggta aatatgaata ttatagaaat tcgctcagat aaaatataca agaagataat
    16321 ggatgcacca ataaacaaaa aagaagatat atacagatat gaattgatga agccttttga
    16381 atttaagtgg aagtgtatga atgttccaat agttgctaga cagaaaggtg gatatgatgt
    16441 aattatagca agtgaaatgt taggggtttt atcgcctaag gatattgatg aaaaacaaaa
    16501 aaagaatata aatgtgttat ctgctgataa aatttgggcc acttgtaaag aaaccataga
    16561 aaactctata aatgctttta taaaagaagg gtatgattta aatattaagg actataaata
    16621 ttcaatatta ttggcgaatc caaatagtcc ttatacaata ttaagtgatg gatactgggg
    16681 tgatggtggg attcctggat atatatttct atcattggtt cctaatgaat atactatcaa
    16741 tagattacca gtattaatag cacatgaatg taatcacaat attagatttc agtttataga
    16801 gtggaataat aatataacat tagaagaaat gatgataaat gaaggtcttg cagaaaattt
    16861 tgcaacatgg atgtttggag aggaaatgtt aggaccttgg gtcagtagaa cagatatcga
    16921 aacattaaat acttatataa agccaataat aaaaagtgct ttaaaagaaa ctggatttca
    16981 aaatataaca tcttatcttt atggtgatga tatagctaaa atgcaaggat attttccagt
    17041 agggttgcct tattgtgcag gatatgcttg tggatattat atgattaagt attatttaga
    17101 aaagacaaat aaatcaataa tcgaagcgac tttattgcct tatagtgaga taatcgaagc
    17161 agtaaaagag ttttgggaat aaattttata aattgcataa tttgaaataa attatgcaat
    17221 tattttattt ttaagaaaaa ataaattgac aataatttat aagaaatata caataatata
    17281 tataaagaaa tatttcaata tttaaaagtt ttaagatttc atatctagag tacctaacat
    17341 tacatgttag tttcttgtta cttatctaat tactttatat ttcgtagtta tatataaaat
    17401 tactataaaa aatgaaatat attaaacaat caactaaaaa atgaaatatt aataaaaaaa
    17461 aattcaaatt ataaatatta aatgaatata attaaaacaa atatataaat ttaagtataa
    17521 cactcacatt ttaatgtatt gtgaaatttt tgttgaactt tatgtaccat aacttataaa
    17581 atttatgtca tgttatattt gaataagtaa taaaattgta gatgtaaatt aatctacaaa
    17641 atttatatga aatggagttg atgctatgac agaattaatg attataagtg ggttagtact
    17701 attgatttgt ataacttcta gcaaggtatt gtataaattt ggagtaccaa tattattgat
    17761 ttttatctta ctagggatgt tatttgggtc tgatgggatg gtaggaatct attttgataa
    17821 ctatgaactg acaaaacaat tatgttctat tggactaata tttataatgt tttttggagg
    17881 gtttggaaca aactggaata tggctaaacc tgttgctata ccttctatat taatgtcttc
    17941 actgggtgta ataattacgg ctggggttac aggtatgttt tgccacatgg taataggaac
    18001 tagtttactt gaaggtttat taattggggc aattgcagct tcaacagatg ctgcatctgt
    18061 gtttgctgtg ttgcgttctc aaaaactaaa cttaaagggt tcacttgcat cattattaga
    18121 agttgaaagt ggaagtaatg acccaatagc gtatatgttg acactaattc ttttgacatt
    18181 gatgaataat tctggggtag aacttattgc accaatggta ataaaacaaa tagcttttgg
    18241 gttagctata ggatttatac tagcaaaatt atctgtttat attttaagac attcaaactt
    18301 cgagatagaa ggattttata ctatatttat aacagctatt gcaatacttt cttatgcatt
    18361 tagcgagtat cttggaggaa atggatattt aagtgtttac atttcaggta ttattatagg
    18421 aaatagtagg atacctctta aaaaaagttt ggttcatttc tttgatggaa tttcatggat
    18481 tatgcaaata atgttattct ttatgttagg attgttatct ttcccttcta agttaccaaa
    18541 tgtatttggt atagcaatgg ccatatctat attcatgata gttattgcaa gaccattagc
    18601 aacatttata gttctagcaa aatttaaatt ctcaaataaa gaaaaattat tcatatcgtg
    18661 ggtaggtctt agaggtgcgg cttcagtagt ttttgcaatt tatgctgtta ctcaaggagt
    18721 agccatagag aatgacatat tccatataat attttttata gcgttgcttt cagtaggaat
    18781 acaaggttca ttgataccaa ctgtagccag aaagttggat ttagttgatg ataatacacc
    18841 tgtattaaaa acatttaatg attatgatgg agaaattagc tctaaactta tcgaagtaaa
    18901 tgttgatgag ggtaataaat gggctaataa gagtataata gattctaata taccagacga
    18961 tatattaata gttatgataa aaagaaagga acaggtttta atacctaaag gttcaaccat
    19021 tataaaaaca ggggatattt tggtattgag tggagataga attgaagaat taatacaatt
    19081 ataatttatt ttgtattgac tttaacctaa ggggaaagtt taaacttaaa ttaatcaaat
    19141 ctaatgcgca ggtgatgaga tgttttctat aggaatgttt tctaaaataa ataaaataac
    19201 tacaaagaca ttaaggtatt atgaagatat agggttgtta aaaccagaat atgtagatga
    19261 atttacaaaa tataggtatt atacaacaga gcaattacct aaattacatc agataataac
    19321 tttaaaacaa atgggtttat cattagctga gataaaaaaa gctgtagaaa atcctgaaga
    19381 agttgaaaat attttaatga ataaagaaaa agaaatgttg gatgtaatta aaagtgaaga
    19441 atataaaata attaaactac gtaattacct tatgaatttg aaaggagaag tcaatatgaa
    19501 tgatgtaatt attaagtctt taccaaaagt taaagttgct tctatgagaa aagtgttaaa
    19561 ggggtatgat gaacttttta atctatttcc aaatgtaatg gctgaagaaa tgatgaaaac
    19621 agggtgtaca tgtgcagttc ctgattattg ttttaatata tatcatgatg gtgaatatag
    19681 agagactgat atggatgttg aaatgtgtca agctataaat gatttaaagg aagatacgga
    19741 tatattaaaa tttaaagaat tagatgcagt tgaaaatgca gtttgtatat tacacaatgg
    19801 tccatattca acattaggaa aaacttacgc agatgcattt aaatggatag aggacaataa
    19861 atataaagtg gttggaaatc ctagagaatc ttatatagat ggtatatgga ataaagagag
    19921 tgaagaagac tggttgacag aagttcagat accagtgaaa cgcatgttat aatatgtcta
    19981 caaagtataa ataagaataa aaaaggtggt gtctcaaagt aaacatttta gtttatgaga
    20041 cactcttttt catatgaaat caagtatatt tttataatta cgattatgaa aaaaggctta
    20101 tctatatttt tagacagcct cttatttttt tattataata gttttctaaa gatgctctat
    20161 ttataaattt ttattaaaga ctttataaat aattgtattt aatatacaaa atatatataa
    20221 atattgatgt tttatatata aagttgtata tttttttaag gatataaagt a
//
LOCUS       DAEPIN010000068        19599 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.068, whole genome shotgun sequence.
ACCESSION   DAEPIN010000068 DAEPIN010000000
VERSION     DAEPIN010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19599
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.068"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            158..349
                     /locus_tag="KR250_002886"
     CDS             158..349
                     /locus_tag="KR250_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0068801.1"
                     /translation="MERLMELIERKFLKDVELLELEDIENVIECKMTQPYNAHYNEYL
                     VKVKNGDEVEEYFVYLKHR"
     gene            complement(438..1250)
                     /locus_tag="KR250_002887"
     CDS             complement(438..1250)
                     /locus_tag="KR250_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HBH0068802.1"
                     /translation="MADCNSCPSKGNCNSQSNCSIENNPNNKFGKIIGVMSGKGGVGK
                     STVTALLANKLNKMGYKVGILDSDITGPSIPRLMGVKNVKAYSDGSYIYPVENSNNIK
                     VMSINLMIDDENEPVVWRGPLLGGVVKQFYTDVLWEELDYLLIDMPPGTGDVALTVMQ
                     SIPISGIVMVSVPQDLVSMIVSKAVNMAKKMNINVLGVIENMSYIQCPDCSKKIKLFE
                     GESTEKFLDDLDLELLGELPMTKEIIDITHNGVTEISDDLDSILTNVVEKIK"
     gene            1672..2823
                     /locus_tag="KR250_002888"
     CDS             1672..2823
                     /locus_tag="KR250_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004452985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBH0068803.1"
                     /translation="MELRPRSAKLEEFDKVIELINYVFRISRNHKPTMMEEFPLLLSK
                     NNIENMIIISEDDKVVSDVNYLIQDVSIQGNRLKVAAIGGVCTHPDYEKRGYSSKILD
                     KVEEKMFYDGVDIVIISGTRSLYSRRNCSLVKSFYKYTIKPEDVKIAYEIVEFDETNF
                     EKDNDLDKMIELYNQNSTRFIRTRDEFQKLLHAATIAWGPIGYKKVFIKENNNIIGYL
                     IIRTIKKEDSTVGEVAEIGLNSVNVENILKYVANKFGLESLNYKVHVKNLKDQLKCNG
                     TKSLDYQQGTMKIINFTKLCDSLRSYFSQYVDFELLKYMEFKQVENKYIIKYKEEELV
                     IENLDKLNKLFFEKNEEQYNEFKHLKNIYEFATKAFPVDFPWTANLNYQ"
     gene            2941..3579
                     /locus_tag="KR250_002889"
     CDS             2941..3579
                     /locus_tag="KR250_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423639.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HBH0068804.1"
                     /translation="MDILDDIAECILNMGVDNIENLVKIAIDKNIDVEDIYEYGLNKG
                     MIGALDKFENKEYYLSEVIVCTDALNKGINLLKGTGKVKKKSKGVILMSVVEGDTHEI
                     GKNIVKVMVEATGYKVIDLGVNRKSEDIIEEAIKNNVDIIGLSSMMTTTMENMKSVID
                     ELNMIEIDKRPKVIIGGGPVSMEFAEEIGADGYSSNAPKAVKLINKLIGGEV"
     gene            3580..4602
                     /locus_tag="KR250_002890"
     CDS             3580..4602
                     /locus_tag="KR250_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HBH0068805.1"
                     /translation="MFSDMTLLQYINSEDRGFFLPDMGTNGLFLINKKAYEVYDSVDL
                     QLELAKTMDKYFESDFIYSFCDGITFCETLGLDTLRPDYDFPSVLTHTITDRDKLRKL
                     DVPDPYTSGRMPLNIESLSVISKSIKKPLYESIQGPFTLAGQLAGATQLLRCIITDKK
                     FVEELLEFTTELVRRYAVAANKAGAKYISIAEPTSVTLSKDRFDEFIVKNLNKIYNEL
                     DCWKGMHICGDTRELLDNMLSCNIDAVSLDQILDYEEIAPMIPRDIVLIGNLDPIKLL
                     GRSKPDKIRRETLKLLKKMRRYDNFLCDFGCNCLNTTPVENLQAAIKAGRISYKELDR
                     IDIDEL"
     gene            4592..5212
                     /locus_tag="KR250_002891"
     CDS             4592..5212
                     /locus_tag="KR250_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0068806.1"
                     /translation="MNYNSNKNKYLLKDINLDDIFIDKNIVLKFLGYGNRKAPDTISK
                     IVEREIRNINDILDIKIYISEVDINKTPKECKKAFAVLYTIGDKIDFKMNDYMENCNM
                     MAGLALDKVGIVCLDCINEKIKMYLKGKYDSLKISHEIYPGDKDFEVERQKDIYNYVK
                     NKYNSIEIEINDYHQLSPIKSVAMLILMGDEENLESRCSKCPRKCF"
     gene            complement(5336..6022)
                     /locus_tag="KR250_002892"
     CDS             complement(5336..6022)
                     /locus_tag="KR250_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HBH0068807.1"
                     /translation="MKTYKFIFFDADDTLFDFKKSESHAFKKLLSEFDLEFNFENYIE
                     SYRNISDKLWLDLEKNIITLNELKLLRFELFANKISLDVDSETLSKMYLNFLGECTFL
                     IPGAIDILQYLKKKYTIVIITNGISAVQKKRLENSKIKEYIDGMVVSEELKISKPNPE
                     IFKYALKKFNCHDKSSALIIGDSLTSDILGGINSGIDTCWLNSNNSINYTNHIPTYEI
                     NTLIELKKLL"
     gene            complement(6036..6581)
                     /locus_tag="KR250_002893"
     CDS             complement(6036..6581)
                     /locus_tag="KR250_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH0068808.1"
                     /translation="MNDLNLGEKIAEVRKKQNLSIRDLAKLADVTPSLLSQIERGLAN
                     PSVNSLKSIASSLNVPLFTFFVSEVDKKNLIVRHDNRKKVILPGSKEVIYEILIPDSS
                     GNLEFAIMDLAPNTSSCVDRITHNGDEIAYVLEGEVKLFMDDDEFTLSKGDSVKVPLG
                     TKHKWQNDSNSESKVIFAVIL"
     gene            6809..8056
                     /locus_tag="KR250_002894"
     CDS             6809..8056
                     /locus_tag="KR250_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HBH0068809.1"
                     /translation="MQITTFGAIFGLLIAIILIIKKFQAVYSLMLGAFIGGLVGGANI
                     TQTVDFMANGAMNISPSILRALASGVLAGSLIKTGAVDKISEQIVKIFGEKRALFSIA
                     ISTMVLAGVGVNLDVSIITVAPIGLYIGRKLNYSKLSILLAMLGGGKAGNIISPNPNT
                     IAVADNFSVNLSSVMMANIIPAIIGVVITVILASILINKGNKVQSYEILEQREDLPSL
                     FKSLCGPIIAIFLLFLGNVSPIVIDPMIALPIGGIVTLIVTGNLNNSREYLAFGLSKM
                     QGVCILLLGTGTIAGIIQMSELQQSTIGALQFLNMPQFLLAPVSGILMSLATASSTAG
                     ATIASSTFHDAIINGGLSPISGASIVNAGSSVFEQLPHGSLFHTSAGSINMDIGERFK
                     LIPYEALIGIVMTIISTSIQLVL"
     gene            8143..10137
                     /locus_tag="KR250_002895"
     CDS             8143..10137
                     /locus_tag="KR250_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBH0068810.1"
                     /translation="MNKKKIVIIGIIYSFLVVFSLTNMYVNMEYNLNVFEYIKKSLPF
                     TEEEKKWLEKHKNLIYSSDQSSPPLRYKGKEDGQYKGIIVDLINSLSIQIGRDFYFKP
                     NNWWKESFVNSIDDSIKFFDLIPSKERANKFIFTDPIYTLSANILKDKKSQDINSYMD
                     LKGKTVAIPEGDYSINFLKQKIQDINILLTPDIKTGVNHLMSGKVDAVVGDEPVLRYY
                     INNYGLSNKYSVLSNPIYTKKAVLAVPKQYEELVSILNKGIFKLQKNGVYKDLKKKWY
                     STYNEVDDILYERGIVPSIYLFIGIILISIYVFYSYTYLLKIEIKRKTEQVIENKKTL
                     EATFNSITDIIMLVDENNNIVESNKVLYDFMGEMSYKIADLISMIKGVIENTFSENTN
                     KTSEIEIHNKILKINTFPVEYKKNNTEYIVVLIKDITNDKIVEAKLLRENKMISIGQL
                     ASGVAHEIRNPLGIIRNNCYLLKDNVTMEEVNDCVKSIESNVDRASNIITNLLNFARI
                     SDDNLEHINIRNFIENIVKLQYKMLQLKNVEIKIDCEHNLVCYINGESLKHVFINLIS
                     NSIDAIHQDGKIIIYCYEKNYCLFIDFKDNGEGIKEDALKDIFNPFYTTKPIGEGTGL
                     GLYITYNEIKKNNGDISVESKLGVGTCFHIKIPLNKEVTI"
     gene            10137..11459
                     /locus_tag="KR250_002896"
     CDS             10137..11459
                     /locus_tag="KR250_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HBH0068811.1"
                     /translation="MKILLVDDELEYGVVMKKILQKKGYLVDIALSGEEAINIIKKDK
                     NYDLVLSDVMMKNMDGVQLLDRIKSINRDIEVILVTGYGSIENAVDAMKRGALSYFIK
                     SNPIENLLEEVEKVKASKISVSVQKNNLEFTLESKNRDFNNIIKIAKKAACKDVNILI
                     LGESGVGKDILARYIHSISPRKNEIFVPVNCCSFSENLLESELFGHEKGSFTGAVDSR
                     KGRFELSNKGTLFLDEIGDIPLNVQVKLLRTLEDKSIERIGSNKSIKVDFRLICAMNK
                     EPKVEISNGNIREDFFYRISTITITIPPLRKRREDLTTLIEFFLNKYQIEHDKKIHSI
                     DKEVKDFLLNYNYPGNIRELKNIINRLVVLSEEGNLSKDNLNLISNNVYIDDKISIRP
                     LREIRKEFECEYIEKVLSLCGNNISNTAKKLEISRRQLTNKISEYNIK"
     gene            11738..13024
                     /locus_tag="KR250_002897"
     CDS             11738..13024
                     /locus_tag="KR250_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006807454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HBH0068812.1"
                     /translation="MKLELGNIFIKNVEFGEKTEVKDGVLYISSEEIEKIVLEDERIV
                     SVNVELARPGESIRIAPVKDVIEPRVKIGDESKIFPGIINKVKTVGSGRTHVLLGACV
                     VTCGNIVGFQEGVIDMSGPTAKYTPFSKTNNICIVIKAKDGIDTHDYEEAARIAGLKI
                     GAYVGEAGREVEPDEVIVYETKPLLKQVKEYPDLPTVAYVHMLQSQGLLHDTYYYGVD
                     AKQIVPTFMYPTEIMDGAIISGNCVAPCDKVTTFHHLNNPVIHDLYKRHGKDLNFIGV
                     ILTNENVFLVDKERSSDMVAKLIEFLGVDGVLVTEEGYGNPDTDLMMNCRKCSEVGAN
                     VVLITDEFPGKDGKSQSIADATKEADAVVSCGQGNLVVHFPAMEKIIGTLDYVEMMIG
                     GYKGCLNEDGSMDAELQIIIASTIANGYNHLTARYY"
     gene            13110..14417
                     /gene="grdB"
                     /locus_tag="KR250_002898"
     CDS             13110..14417
                     /gene="grdB"
                     /locus_tag="KR250_002898"
                     /EC_number="1.21.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:14157..14159,aa:Sec)
                     /transl_table=11
                     /product="glycine reductase complex selenoprotein B"
                     /protein_id="HBH0068813.1"
                     /translation="MSKIRVVHYINNFFAGIGGEEKADIPPEKRAGVVGPGIAFQSQF
                     NDDAEIISTVICGDTYFGENIESATQELLKMIKEESPDLFIAGPAFNAGRYGVACGTI
                     CKAVEEELNIPVITGMYKENPGVDMFKLDLHIVSTGNSAASLRKVVPIMTKLGLKLVR
                     GEEIGPPEEEGYIMRGIRKNFFHELRGSERAIDMLVKKMKGESFETEYPMPEFDRVTP
                     MKPVKDLSKIKLALVTSGGIVPIDNPDKIESSSATKYGIYDLTDMDSMSNKEFTTIHG
                     GYDRAYVLENPNLVVPLDVVRELEKEGVIGELVDYFITTTGTGTSVGNSKRFGEEFSK
                     KLLEDDVDAVILTSTUGTCTRCGATMVKEIERAGIAVVHICTVVPISLTIGANRIVPA
                     VGIPYPLGNPMLGEEESKKIRKNIVLKALNALTEDIEEQTVFE"
     gene            14695..15426
                     /locus_tag="KR250_002899"
     CDS             14695..15426
                     /locus_tag="KR250_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0068814.1"
                     /translation="MIRKKVYILISIAICDDESYMLNHLKENIELYMKQKNLLYNIEL
                     FNKVEDLLCNHEKFNIVFLDIQMEEINGMQAARKLRSYGNECFIIFVTVLNEFVFDAF
                     EVDAINYLIKPLSNEKLYKTLDKIVTKLDDNESNYITIQKGYSYWKLKLADILYCEVI
                     KRKIYVHLKKETVDYYETIENLERSLPTNFFRCHRSYIINLQYVYGYENGNAVMESGD
                     RIPVSRLRQQIFSKVMLEFMKNRRI"
     gene            15428..16786
                     /locus_tag="KR250_002900"
     CDS             15428..16786
                     /locus_tag="KR250_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBH0068815.1"
                     /translation="MINLFSSDLFLKYSFLFSFIVYRILMVIVQYYFLISFTNVNSKF
                     WCFILYSIISIFLIIINTYFNLYNLFSSQILFFLMTFLFVKYIIKQSTVLSIVSTLLV
                     YLIEQLVYGFTAPLNYLIFTSDINTDIYKFDINMGLSSLITIIIAGFIYWYSSKKFNI
                     KVMNFDKYIAILMIPLLLIILFMQSFEYSSNINIDTSLGIVKLLLNTSITEEIQAFLF
                     SIIGTICVFFSLFTFKKLIQALEDDKERAIMNQQIHAQKNYIEEAKSRLSQTISFRHD
                     FNNHLAIVNGLLKKDQILKAQDYLNKLEKVTSDLSFSCNTQNIVIDALFNNKLSIAKQ
                     LGIEVDCEVIIPQSTNMNDFDLCIVFANAIDNAIKACRVVDKQNRYLKLSTKLEGGFF
                     MIEIKNSYNPINNYTSGSGIGLLNIKEIAKKYQGAISIDKTSDYFQINVLLVISLHCA
                     SI"
     gene            16825..17310
                     /locus_tag="KR250_002901"
     CDS             16825..17310
                     /locus_tag="KR250_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0068816.1"
                     /translation="MYLNRESLIYRNEEVTKMKVGNINGQAPITNGLDSCQSEVQGLE
                     QKKKSLQDEIEKVRSDKSIDGETAKLKIEALKKEMEEIENKIQELKGNKAKKTNKEEN
                     NVSEVNNNLLERKKEPSNNENVKQLYDVFERSNVDEQWDNTYRVLQDDKNLKVKFNRP
                     I"
     gene            complement(17573..17809)
                     /locus_tag="KR250_002902"
     CDS             complement(17573..17809)
                     /locus_tag="KR250_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0068817.1"
                     /translation="MLLTHIYFNGQFKQTIELYKQAFNATVKTLIEDSCYLLIVHNEI
                     VIHNKFLILNDFGNKNGVSISEGYQPTDKIQTAS"
     gene            complement(17964..18188)
                     /locus_tag="KR250_002903"
     CDS             complement(17964..18188)
                     /locus_tag="KR250_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBH0068818.1"
                     /translation="MTVYNLKLGQKGIIDNIAGNEKLMKRLLALGLIDGTEVEVKKIA
                     PLNDPIVIRFRGFDLAIRKSDAKNINLKNN"
     gene            complement(18285..19538)
                     /locus_tag="KR250_002904"
     CDS             complement(18285..19538)
                     /locus_tag="KR250_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:glutamate symporter"
                     /protein_id="HBH0068819.1"
                     /translation="MITLSFDIVQTLTLSIFFFLIGNLLKNKVNFLNNFCIPAPVIGG
                     LLFCFLNLFFKYFNIADISISGNLMPNFITFFFTTIGLEISINLIKKGGSVLFRYWIL
                     CGVLAFCQNILAITISKIIKLEPLLGLMCGNVSMEGGHGYSAAFGLTIENLGIEGAVG
                     VGLSAATIGLIMGGILGCPVAKFLINKYKLKPSSNIDLSIPRYNRNLKRFGSKFFRNK
                     NRIKNNNFSETITPSVFLEQVLLIFICINTGEIISRCFYITFNILLPSVVTCMFSAVI
                     FRNLNDKINILELNFKLIDFLKELSLGIFLTLSLMNIDLFELSTLLPPILLIVTFQVI
                     FIILFSIFICFRVLGKDFDSAIIISGLIGHGIGATPNALANMSSLTQKYGDSPKAFLV
                     VPLVSGFLLDAISIPCILFFINILT"
BASE COUNT         7789 a         2123 c         3134 g         6553 t
ORIGIN      
        1 aaggcatatt tattaaaaaa tcaatctcta tttgttaaaa taatttatga atgatgttag
       61 aaactgtaat atattggata taatataata aacagataaa ctctgcatta agaggatata
      121 tagtttatct taattatagg ggaagtgagg atatattatg gaaagattaa tggaactcat
      181 agaaagaaaa tttttaaagg atgtagaact tttagaatta gaagacatag aaaatgtaat
      241 agaatgcaaa atgacacagc catataatgc tcattataat gaatatttag taaaagtaaa
      301 aaatggagat gaagtagaag aatattttgt gtatttaaaa catagataaa cataaaagag
      361 gtttatattt atttgtatga ttataaaata aatacaagcc tctttttttg ataaatataa
      421 aattataaaa tttttattta tttaattttt tcaacaacat tagtcaatat tgaatctaaa
      481 tcatcactaa tttctgtaac tccattatga gtaatatcta taatttcttt agtcatagga
      541 agttctccta aaagctctaa gtctaaatca tctaaaaact tttcagtaga ttctccttca
      601 aataacttga tttttttaga acaatcagga cattggatat aactcatatt ttcaataact
      661 ccaaggacat ttatgttcat ttttttagcc atattaacag cttttgatac aatcatagat
      721 accaaatctt gaggtactga aaccataact attccactta taggaataga ttgcataact
      781 gttaaggcaa catctccagt accaggaggc atatctatta ataaatagtc taattcttcc
      841 caaagtacat cagtataaaa ttgctttaca acccctccaa gtaaaggtcc tctccaaaca
      901 acaggttcat tttcatcatc tatcataagg tttatagaca ttacttttat gttatttgag
      961 ttttctacag ggtaaatata agagccatca gaataagctt ttacattttt tacacccata
     1021 agtctaggaa tacttggtcc agttatatct gagtctaata taccaacctt atatcccatt
     1081 ttgtttagct tattagcaag taaagcagtt acagttgatt ttccaactcc acccttccca
     1141 ctcataacac caattatttt tccaaattta ttgttagggt tattttcaat agaacaattt
     1201 gattgactat tacaatttcc ctttgatgga catgaattac aatctgccat atctattacc
     1261 tccaaacttt aatatttatt agattatttg taacctacat atactatttt agtacaattg
     1321 aaccataaag tcattattta aaagcaactt agaagatttt ctattgattt attcatagac
     1381 tatagcaaaa agaatacaat aaaaaaatta taacattctt gaaaaagtga ttatttcaat
     1441 ttagttatag aacttagtca tataaatata tagaaaatat tttaatatat tatacataac
     1501 gggttatttt ataagtttaa ctataaatta ggtttatatt ttataaaaat ttgctaaaaa
     1561 aaacaattaa aaattgtaaa gaaaaaagat ataattagaa ctgtagtaat aaatgtttta
     1621 ttatagataa tagagtttta agtatcttaa ataatatttg gaggaatatg aatggaatta
     1681 agaccaagaa gtgcaaaatt agaagaattt gataaagtaa tagaattaat aaactatgta
     1741 tttagaatat caagaaatca taaaccaact atgatggaag aatttcctct tttactaagt
     1801 aaaaataata tagaaaatat gattataata tcagaagatg ataaagtggt ttctgatgta
     1861 aactatctaa ttcaggatgt atctattcaa ggaaacagat tgaaggttgc agctataggt
     1921 ggagtatgta ctcatccaga ttatgaaaag agaggctatt catctaagat attggataaa
     1981 gtagaagaaa aaatgtttta tgatggtgta gatattgtaa ttatatcagg aacaagaagt
     2041 ctatacagta gaagaaattg tagtttggta aagagttttt ataaatatac aataaaacct
     2101 gaagatgtaa aaatagcata tgaaatagtt gaatttgatg aaactaattt tgaaaaagat
     2161 aatgatttag ataaaatgat tgaactatat aatcaaaata gtactagatt tataagaacg
     2221 agagatgagt ttcaaaaact attgcatgca gcaactattg cctggggacc aattggttac
     2281 aagaaggtat ttataaagga aaataataat ataataggat atttgataat aagaacaata
     2341 aaaaaagaag attctactgt aggagaagtt gctgaaatag ggttaaactc tgtaaatgtt
     2401 gaaaatatat tgaaatatgt tgcaaataaa tttggattag aatctttaaa ttataaagta
     2461 catgtgaaaa atcttaaaga ccagttgaaa tgtaatggga caaaatcttt ggattatcaa
     2521 caaggaacta tgaaaataat aaattttact aagttgtgtg atagtttaag aagttatttt
     2581 agtcaatatg tagattttga attactaaaa tacatggaat ttaaacaagt tgaaaataaa
     2641 tatattatta aatacaaaga agaagaactt gtaatagaaa atttggataa gcttaataaa
     2701 ctgttttttg aaaaaaatga ggaacagtac aatgaattta aacatctaaa aaatatatat
     2761 gaatttgcta caaaggcatt tcctgtagat tttccatgga ctgcaaattt aaattatcaa
     2821 tagttttaga tgatattaaa taaaattgat tgatacctag agttagtcct tgttaaataa
     2881 gagaaattat tggatattga aaaagcaatt ttgtaagtaa ttagaaagga tgagtattat
     2941 atggacatac ttgatgatat agcagaatgt atattaaata tgggtgtaga taatatagag
     3001 aatttagtaa aaattgctat agataaaaat attgatgtgg aagatattta tgaatatggt
     3061 ttaaacaaag gtatgatagg agctttagat aagtttgaga ataaggaata ctatctttca
     3121 gaagttatag tatgcacaga tgcattaaat aaaggtatta atcttttgaa gggaactgga
     3181 aaagtaaaga aaaaatcaaa gggagtaatt ctgatgtctg tagtagaagg agatactcat
     3241 gagataggaa aaaatattgt aaaagtaatg gtagaagcca ctggatataa ggttattgac
     3301 ttaggtgtaa atagaaaaag tgaagatata atagaagaag ctataaaaaa caatgtagat
     3361 ataataggct tatcttcaat gatgacaact acaatggaaa atatgaaatc ggttatagac
     3421 gaacttaata tgattgagat agataaaaga ccaaaagtaa taattggtgg aggacctgta
     3481 agtatggagt ttgcagaaga aataggagca gatggataca gttcaaatgc tccaaaagca
     3541 gttaaattaa taaataaact tataggaggt gaagtttaaa tgttttcaga tatgacttta
     3601 cttcagtata taaatagtga agatagaggt ttttttctgc ctgatatggg tacaaatgga
     3661 ttgttcttaa taaataaaaa ggcatatgaa gtatatgata gtgtagattt gcaattagaa
     3721 ttagcaaaga ctatggacaa gtattttgaa tcagatttta tatactcatt ctgtgatggg
     3781 attacatttt gtgaaactct gggtttagat acattaagac cagattatga ttttccaagt
     3841 gtattgacac atactataac tgacagagat aaattaagaa aattagatgt tccagaccct
     3901 tatacgagtg gacgtatgcc actcaatata gagagcttat cagttatatc aaaaagtatt
     3961 aaaaaaccac tatatgaatc gatacaaggt ccatttacac ttgcaggtca attagctgga
     4021 gctactcaac ttttaagatg tataataact gataaaaaat ttgtagaaga attattggaa
     4081 tttacaactg aattggtaag aagatatgct gtagctgcaa ataaagcagg agcaaaatat
     4141 atttcaatag cagagccaac atcagtaacc ctaagtaaag atagatttga tgaatttata
     4201 gtaaaaaatt taaataaaat ctacaatgaa cttgattgtt ggaaaggtat gcatatatgt
     4261 ggagacacca gagaattatt agataatatg ctaagttgta atatagatgc agtaagttta
     4321 gaccaaattt tagactatga agaaattgct ccaatgattc ctagagatat agttttgatt
     4381 ggtaatttag acccaataaa acttcttgga cgttctaagc cagataagat tagaagagag
     4441 acactaaaat tgttaaagaa gatgagaaga tatgataact ttttatgtga ttttggctgt
     4501 aactgcttaa ataccactcc agtagaaaat ttacaagcag ctataaaggc aggaagaatt
     4561 agttataaag aattagatag gattgatatt gatgaattat aatagtaata aaaataaata
     4621 tcttttaaag gatataaatt tagatgatat ttttatagat aagaatattg tgctaaagtt
     4681 tttggggtat ggaaatagaa aagctccaga tacaattagt aaaatcgttg aaagagaaat
     4741 aagaaatata aatgatattt tagatatcaa gatatatata agtgaagttg atattaataa
     4801 aacacctaaa gaatgtaaaa aagcatttgc tgtactatat acaatagggg ataaaattga
     4861 ttttaagatg aatgactata tggaaaattg caatatgatg gcagggctgg cattggataa
     4921 ggtaggtata gtatgtttag attgtattaa tgagaaaata aaaatgtatt taaaaggaaa
     4981 gtatgatagt ctcaaaatat cgcatgaaat atatccagga gataaagatt ttgaagtaga
     5041 gagacaaaag gatatttata actatgtaaa aaataaatat aatagtatag aaatagaaat
     5101 taatgattat catcaactat cccccataaa aagtgttgca atgcttattt taatggggga
     5161 tgaagaaaat ttagaatcaa gatgtagtaa gtgtcctaga aaatgctttt agttaatttt
     5221 caagactaag atttgttttg tgtataaatt gcaataattt ttttggtcat tttatgtata
     5281 aactaaaaaa attattgcaa agtaatattc tatttaattt gaacagactt acaaactata
     5341 gtagtttctt taattcaata agtgtattta tttcataagt tggtatatga ttagtataat
     5401 ttatactgtt attggaattt aaccaacaag tatctattcc tgaatttatt ccacctaaga
     5461 tatcagaggt taagctatcc ccaattatta aggcagaaga tttatcatga caattaaact
     5521 ttttgagagc gtatttaaat atttcaggat ttggttttga tattttcaac tcttcagata
     5581 caaccattcc atcaatatat tcttttattt ttgaattttc aagtcttttc ttttgaactg
     5641 cagaaattcc atttgttatt ataacaatag tatacttttt tttaagatat tgtaatatat
     5701 cgattgcacc tggaattaaa aatgtacatt cacctaaaaa atttaagtac attttgctaa
     5761 gagtttcaga atcaacatcc aaagatattt tatttgcaaa aagttcaaat ctaagtaatt
     5821 taagttcatt caaagttata atattttttt caaggtcgag ccaaagctta tcacttatat
     5881 ttctatatga ctctatataa ttctcaaaat taaattctaa atcaaactca gatagaagtt
     5941 ttttaaaagc atgtgattca cttttcttaa aatcaaataa agtatcatct gcatcaaaaa
     6001 atataaattt atatgtcttc atggttttta ctcctttaca agatgactgc aaaaattact
     6061 ttactttcac tatttgagtc attttgccat ttatgttttg ttcctaaagg aacttttaca
     6121 ctatctcctt tggacaaagt aaattcatca tcatccataa aaagtttaac ttctccctcc
     6181 aaaacatatg caatttcatc tccattatga gtaatcctat caacacaaga agaagtgttt
     6241 ggagctaaat ccataattgc aaattctaaa ttaccactag agtctggaat taaaatctca
     6301 tatataactt ctttgcttcc aggtaaaata actttttttc tattatcatg tcttactatc
     6361 aagttttttt tatctacctc agatacaaaa aatgtaaata aaggaacgtt tagagaagag
     6421 gctatagatt ttaatgaatt tacagatgga ttagcaagac ctcgttctat ttggctcaat
     6481 aatgatggtg taacatcagc tagttttgct aaatctcgaa tacttaagtt ttgttttttt
     6541 cgaacttcag ctattttttc ccctaaattt aaatcattca tataaatatc ctctcttttt
     6601 aatttaaaaa atattaactt aaaataatca agtaaaatga ttatgtagta tgaaattatt
     6661 ataacatata gaattaggtt ttgaaaaaac tatgttcaat ataattaaat aaattaaaat
     6721 ataattgaca aagtggatac agaatactta aaatatattt aaataaaatt aagcaaaaga
     6781 tagttaattt atgataggag gggatattat gcaaataacg acttttggag ctatatttgg
     6841 tttattaata gcaattattt taattataaa aaaatttcaa gctgtatata gtttaatgct
     6901 aggagcattt attggaggat tagttggagg agcaaatata acacaaactg tagattttat
     6961 ggcaaatgga gctatgaata tatcaccatc aattcttaga gctttggcat caggtgtatt
     7021 agctggaagt ttaataaaaa caggggcagt agataaaata tcagaacaga ttgtaaaaat
     7081 atttggtgaa aagagggctt tattttcgat tgccatttca actatggtac ttgctggagt
     7141 aggcgtaaat ttggatgtat ctataataac tgtagcacct ataggattat atataggtag
     7201 aaaattaaat tattctaaat tgtcaatact attagctatg ttgggagggg gtaaagcagg
     7261 taatataata tcaccaaatc caaatactat agcagtagca gataattttt ctgtcaatct
     7321 ctctagtgtt atgatggcaa atataattcc agcaattatt ggagttgtta ttacagttat
     7381 tttagcaagt atattaataa ataaaggaaa taaagtacaa tcatatgaaa ttttagagca
     7441 aagagaagat ttaccaagtt tatttaaatc attatgtggt ccaattatag ctatatttct
     7501 attgttttta ggaaatgtat ctcctatagt tatagaccca atgatagccc ttccaatagg
     7561 aggaatcgta actctaattg tcacaggaaa cttgaacaat tcaagagaat atttagcttt
     7621 tggattatct aagatgcaag gggtatgcat attattactt ggaacaggga ctatcgcagg
     7681 aattatacag atgtctgaat tacaacagag tacaataggt gctttacaat ttttaaatat
     7741 gccacaattt ttattggcac cagtttcggg tatacttatg tcattagcaa cagcatcatc
     7801 aacagctggt gctactatag catcatctac ttttcatgat gctataataa atggtgggct
     7861 atcaccgatt tcaggtgcct caatagtaaa tgctgggtct agtgtattcg agcaattacc
     7921 acatggctca ctatttcata ctagtgcagg tagtataaac atggatatag gtgaaagatt
     7981 taagttaatt ccatatgaag cattgatagg aatagtaatg actataattt caacatctat
     8041 tcaattagtt ttataataaa tataaatatt taatgaaaaa agtaggattt tgttattaaa
     8101 tattgaattt attatagtaa aaaagaagat tggagtttta aagtgaataa aaaaaagatt
     8161 gttattatag ggattattta ttcattttta gtagtatttt cacttacaaa tatgtatgta
     8221 aatatggagt ataatctaaa tgtatttgaa tatattaaga agtcacttcc atttacagaa
     8281 gaagaaaaaa agtggttaga aaaacacaag aatttgattt acagctctga ccaaagttca
     8341 cctcctttga gatataaagg taaggaagat ggacagtata agggaattat tgttgattta
     8401 ataaattctc tgtcaattca aattggtaga gatttttatt ttaaaccaaa taattggtgg
     8461 aaagaatctt tcgtaaattc aatagatgat agtataaagt tttttgactt gattccatca
     8521 aaagaaaggg caaataaatt tatatttact gacccaatat acactttgag tgcaaatata
     8581 ttgaaagata aaaagtccca agatataaat agttacatgg atttaaaggg gaaaacagta
     8641 gcaattccag aaggtgatta ttctataaat tttttaaagc aaaaaattca agatataaac
     8701 attttgttga ctccagatat aaagactgga gtcaaccatt taatgtctgg aaaagtagac
     8761 gcagttgttg gtgatgaacc agtattgcga tactatataa ataattatgg actttcaaat
     8821 aaatacagtg ttttaagtaa tcctatatat actaaaaaag cagttcttgc tgtacctaaa
     8881 caatatgaag agctggttag tattttaaat aaaggcattt ttaaacttca gaaaaatgga
     8941 gtgtataaag atttaaagaa aaaatggtat tctacgtata atgaagttga tgatatattg
     9001 tatgagcgag gaattgtgcc tagtatttat ctttttatag gtataatttt aatatcaatt
     9061 tatgtttttt atagctatac atatttgtta aagatagaaa tcaaaaggaa aacagaacag
     9121 gtaatagaaa ataaaaaaac tttagaggca acttttaata gtattacaga tataataatg
     9181 cttgtagatg aaaataacaa tatagtagaa tcgaacaaag tactatatga ctttatgggt
     9241 gaaatgagtt ataagatagc agatttgatt agcatgataa aaggtgttat tgaaaatacc
     9301 ttctctgaaa atactaataa aactagtgaa atagaaatac acaataaaat tttaaagata
     9361 aacacttttc ctgtagagta taaaaaaaat aatactgagt atatagtagt tcttattaaa
     9421 gatataacta atgataaaat agtagaggcg aaattactta gagaaaacaa aatgatatct
     9481 ataggacaat tagcttctgg ggtagcacat gagataagaa atcctttggg tataataaga
     9541 aataattgct atctcttaaa agataacgtt acaatggaag aagtaaatga ttgtgttaaa
     9601 tcaatagaaa gtaatgtaga tagggcaagt aatataataa ccaacttgtt aaatttcgca
     9661 agaatatcag atgataattt agaacatata aatataagaa attttattga aaatatagtg
     9721 aaacttcaat ataaaatgtt acagttaaag aatgttgaaa taaaaattga ctgtgaacat
     9781 aatttagtat gctatataaa tggagaatcg ttaaaacatg tatttataaa tttaatttct
     9841 aattcaatag atgcaataca tcaagatgga aaaattatta tatactgtta tgaaaaaaat
     9901 tattgtttat ttatagattt taaagataat ggtgaaggaa taaaagaaga tgccctaaag
     9961 gacatattta atcccttcta tacaacaaaa ccaattggtg agggaactgg tttaggttta
    10021 tacattactt ataatgagat taaaaagaat aatggagata taagtgttga aagtaaattg
    10081 ggggttggaa cttgttttca tataaaaatt ccattaaata aagaggtgac tatataatga
    10141 aaatactatt agttgatgat gagctagaat atggagtggt aatgaaaaaa attctacaaa
    10201 agaagggcta tcttgtagat atagcactaa gtggtgaaga agctataaat ataattaaaa
    10261 aagataaaaa ttatgatttg gttttgtctg atgttatgat gaaaaatatg gatggagtac
    10321 agctattaga tagaattaaa tctattaata gagatataga ggttatatta gttacaggat
    10381 atggaagtat tgaaaatgca gttgatgcta tgaaaagagg agctttatct tattttataa
    10441 aaagtaatcc cattgaaaat ttattagaag aagttgaaaa ggtaaaggca agtaaaatat
    10501 ctgtttctgt acaaaaaaat aatctggaat ttactttgga atctaaaaat agagatttta
    10561 ataatatcat aaaaatagca aaaaaagctg cttgtaaaga tgttaatata ttaatcttgg
    10621 gggaatctgg tgtaggaaaa gatatattag ctagatatat acatagcata agtccaagaa
    10681 agaatgagat atttgtacct gtaaattgtt gttctttttc agaaaatcta ttggagtctg
    10741 agttgtttgg tcatgaaaaa ggttctttta caggagcagt agatagtaga aaaggtagat
    10801 ttgaactttc gaataaagga actctatttt tggatgaaat aggagatata cctctaaatg
    10861 ttcaggtaaa acttttgagg acacttgagg acaaatctat agagaggata ggaagtaata
    10921 aatctattaa agttgacttt aggttaattt gtgctatgaa taaagaacca aaagtggaaa
    10981 tatcaaatgg aaatataaga gaagattttt tttatagaat aagtacaata acaataacta
    11041 taccaccgct aagaaagaga agagaagact taactactct tatagaattt ttcttaaata
    11101 agtaccaaat tgaacatgat aaaaagatac actcgattga taaggaagta aaggattttt
    11161 tattaaatta caattatcca ggaaatataa gagaattaaa aaatataata aatagacttg
    11221 tagttttaag tgaagaaggc aatttatcaa aagacaattt aaatttaata tcaaataatg
    11281 tatacataga tgataaaata agtattagac ctttaagaga aataagaaaa gagtttgaat
    11341 gtgagtatat tgaaaaagtt ttatctttgt gtggaaataa tatttcaaat acagctaaaa
    11401 aattggagat tagtagaaga cagttaacca ataaaatatc agagtataat attaagtgag
    11461 aaaaaaactt ctcattaagt gagaaaaaaa tttctcaaac tgtattaaaa gataaacaga
    11521 aaaaatctgt ttatcttttt ttattataat tttaaaaatt aatatttaca ataattattg
    11581 ctaaattcaa tattggtgta aattaaaaat gaaaatttct atgacattaa tacatagaat
    11641 atatatttta aataattggc atagaaaatg ctttatatat taataaacgt aataaatact
    11701 tttaattgcg attaaatata ttaaggggga ttttcatatg aagctagagc tagggaatat
    11761 atttataaaa aatgttgaat ttggagaaaa gacagaagta aaagatggag tactatatat
    11821 tagtagcgaa gaaatagaaa agatagtatt agaagatgaa aggattgtaa gtgtaaatgt
    11881 tgaacttgca agacctggcg aatcaataag aatagctcca gttaaagatg taatagagcc
    11941 tagagttaaa attggagatg aaagcaaaat atttcctgga attataaata aagtgaagac
    12001 tgtaggaagt ggaagaaccc atgtactttt aggagcttgt gttgtaactt gtggaaatat
    12061 agttggattt caagaaggcg taattgatat gagtggtcct actgctaaat atacaccttt
    12121 ttcaaaaaca aataatatat gtattgttat aaaagcaaag gatggtatag atactcatga
    12181 ttatgaagaa gcagctagaa ttgcaggatt aaaaataggt gcatatgtag gtgaggcagg
    12241 aagagaagtt gagccagatg aagtcattgt atatgagaca aagccattat taaaacaagt
    12301 aaaagaatat ccagacctgc caacagtagc atatgttcac atgttacaat cacaaggact
    12361 tttacatgac acttattact atggggtgga tgctaaacag atagtgccta catttatgta
    12421 tccaactgaa ataatggatg gagctataat atctggaaac tgtgtagcac catgtgataa
    12481 ggttacaaca ttccatcatt taaataatcc agttatacat gacttatata aaagacatgg
    12541 aaaagattta aactttatag gagttatttt gactaatgag aacgtattct tagtcgataa
    12601 agagagaagc tctgatatgg tagctaaatt aatagaattt ttaggagttg atggagttct
    12661 agtgacagaa gaaggttatg ggaatccaga tactgaccta atgatgaatt gtagaaaatg
    12721 tagtgaagtt ggagcaaatg tagttttgat aacggatgaa tttcctggaa aagatggcaa
    12781 gagtcaatct atagcagacg ctactaagga agcagatgca gttgtttctt gtggtcaagg
    12841 aaatctagtg gttcatttcc cagcaatgga aaaaattata ggaactttag attatgttga
    12901 aatgatgata ggtgggtata aaggatgctt aaatgaagac ggaagcatgg atgcagagct
    12961 acaaattata atagcatcaa ctatagcaaa tggctataat catttgacag caagatatta
    13021 ttaaataagt atttgaatat aaagttctgt tgatataatc attggtctaa ttgatgtaac
    13081 aactataaag tgatttggag gattaagaga tgagtaaaat cagagtggtt cattatataa
    13141 acaatttctt tgctggtata ggtggagagg aaaaagcaga tattccgcca gaaaaaaggg
    13201 ctggtgtagt aggacctgga atagcatttc agagtcaatt taatgatgat gcagagataa
    13261 tctcaactgt aatttgcgga gatacgtatt ttggtgaaaa tattgagagt gcaactcaag
    13321 aacttttaaa aatgataaaa gaagaaagcc ctgatttatt tattgcaggc ccagcattta
    13381 atgcaggtag atatggagta gcttgtggga ctatttgtaa ggcagttgaa gaagagttaa
    13441 atattcctgt aattacaggc atgtataaag aaaatccagg tgtagatatg tttaaattag
    13501 atttgcatat agtgtctaca ggaaattcag cagcaagtct tagaaaggta gttccaataa
    13561 tgacaaaact aggtcttaaa ttggttagag gtgaggaaat aggacctcct gaagaggaag
    13621 gatatataat gagaggaata agaaaaaatt tcttccatga acttagaggg tctgaaagag
    13681 caattgatat gttggttaaa aagatgaaag gggaaagctt tgaaactgag tatccaatgc
    13741 ctgaatttga tagagttaca cctatgaaac ctgttaaaga tttatctaag ataaaacttg
    13801 ccctagtaac ttctggaggg atagttccaa ttgataatcc agacaaaatt gaatcttcaa
    13861 gtgctactaa atatggaatt tacgatttaa cagatatgga ttctatgtca aataaagaat
    13921 ttacaactat acatgggggg tatgacagag catatgtcct tgaaaatcca aatttagttg
    13981 taccactaga tgtagttaga gagcttgaaa aagaaggtgt aattggagaa ttagtagatt
    14041 attttataac tactacagga acaggaacga gtgttggcaa ttctaagaga tttggagaag
    14101 agttttctaa aaaattgtta gaagatgatg tagatgctgt aattttaact tctacatgag
    14161 gtacttgtac tcgttgtgga gcaacgatgg taaaagagat tgagagagct ggtatagcag
    14221 ttgttcatat atgtacagta gtaccaatat cacttacaat aggagctaat agaattgttc
    14281 cagcagtagg gataccatat ccacttggaa atcctatgtt aggagaagag gaaagtaaaa
    14341 aaattagaaa gaatatagtt ttaaaagctt taaatgcact aacagaagat atagaagaac
    14401 aaacagtatt tgaataataa aaatacaaca caacactgaa ataaaaaaag atatcaaaaa
    14461 ataatttttt aaaattattt tacgatatct tttttttatt aggtaaattc gaaatttgca
    14521 ttttttaaaa ttaaatttat aaaagtgata aattagttta ttataatatt gatacacaaa
    14581 ttcaaattag atatactatg aaaagcgaga ttattacatt ttggaacaaa tattcaacat
    14641 attagatcat attaattgaa cagaaaaaat taactttata taataattag aataatgata
    14701 agaaagaagg tgtatatatt gatttcaatt gccatttgtg atgatgaatc ctatatgcta
    14761 aatcatttaa aggaaaatat agaattatac atgaagcaga agaatttatt atataatata
    14821 gagcttttta ataaagtaga agatttacta tgcaatcatg aaaagtttaa tattgtattt
    14881 ttagatatac agatggaaga gattaacgga atgcaggcag ctagaaaatt aaggtcatat
    14941 ggtaatgagt gctttataat tttcgttaca gttttaaatg aatttgtatt tgatgctttt
    15001 gaagtagatg ctataaatta tctgataaaa cctctatcta atgagaaact atataaaact
    15061 ttggataaaa tagttacaaa acttgatgat aatgaaagta attatatcac aatacaaaaa
    15121 ggttattcat attggaaatt aaaacttgct gatattttat attgtgaagt tataaaaaga
    15181 aagatttatg ttcatttaaa gaaagagact gtagattatt atgagactat tgaaaattta
    15241 gagagaagtt taccaacaaa tttttttagg tgtcatcgta gttatataat caatttacaa
    15301 tatgtatatg gatatgaaaa tggtaatgca gttatggaaa gtggagatag aatacctgtt
    15361 tctagactac gtcaacaaat attttcaaaa gttatgcttg agttcatgaa aaataggagg
    15421 atataagatg attaatttat tttcttccga tttattttta aagtacagtt ttttattttc
    15481 ttttattgta tatagaattt taatggtaat agtgcaatac tattttctaa tcagttttac
    15541 aaatgttaat tcaaaatttt ggtgttttat tttatattcg ataatttcaa tttttttaat
    15601 cattataaat acatatttta atttatataa tttattttct tcacagattc tattttttct
    15661 tatgactttt ttatttgtaa agtacataat aaaacaatct actgttttat ctatagtatc
    15721 aactttgtta gtatatttaa ttgaacaatt agtatatgga tttacagctc cattaaatta
    15781 cttgatattt actagtgata taaatacaga tatttataaa tttgatataa atatgggact
    15841 atcttcttta attactatta taatagctgg atttatatat tggtatagtt ctaaaaagtt
    15901 taatattaag gtcatgaact ttgataaata tatagcaatt ttgatgattc cattgttgct
    15961 tataatatta tttatgcaat catttgaata tagttcaaat ataaatatag atacaagtct
    16021 tgggattgta aagctgctat taaatacttc tataactgaa gaaattcaag cttttctttt
    16081 ttctataatt ggaactatat gtgtattttt ttctctattt acctttaaga aactaattca
    16141 agctctcgaa gatgacaagg agagggcaat tatgaatcaa cagattcatg ctcaaaaaaa
    16201 ctatattgaa gaagcgaaat cacgattatc acaaactata tcctttagac atgattttaa
    16261 taatcattta gctattgtca atggattgtt gaaaaaagac caaattttaa aagcacaaga
    16321 ttatttaaat aagcttgaaa aagtaacaag tgacctatca ttttcttgta atacacagaa
    16381 tattgttatt gatgctcttt tcaacaataa attaagtata gcaaaacaat taggcataga
    16441 ggtagattgt gaagtaatta taccccaaag cactaacatg aacgattttg acctatgtat
    16501 agtgtttgct aatgctattg acaatgcaat taaagcttgt agagttgtag ataaacaaaa
    16561 taggtattta aagctttcca ctaagttaga gggtggattt tttatgatag aaataaaaaa
    16621 tagctataat ccaataaata attatacaag tggctcaggt ataggtcttt taaatataaa
    16681 agaaattgct aaaaaatatc aaggggctat aagtatagat aagacatcag attattttca
    16741 aataaatgta ttgctggtta tttcattaca ttgcgctagt atttgataac aatttcattg
    16801 aaaatagtat ttgtaagcta tattatgtac ttgaatagag aaagtttgat atatagaaat
    16861 gaggaggtta ctaaaatgaa agttggaaat ataaatggtc aagcaccaat tacaaatggt
    16921 ttagatagtt gtcaaagtga ggtacaaggc ttagaacaaa agaaaaaatc attacaggat
    16981 gaaatagaga aagtaagaag tgataaaagt attgatggtg aaacagccaa attaaaaata
    17041 gaagctttaa aaaaagaaat ggaagagatt gaaaataaaa ttcaagagtt aaaaggaaat
    17101 aaagcaaaaa aaacaaataa agaagaaaat aatgtttcag aagtaaataa taatctatta
    17161 gagagaaaaa aagaaccttc taataatgaa aatgtaaaac aattatatga tgtttttgaa
    17221 agaagcaatg tagacgaaca atgggataat acttatcgtg ttctccaaga tgataaaaac
    17281 ttaaaagtaa aatttaatag acctatatag tttatttata aaaatagtta atattttaat
    17341 tatcaccact ttttagaaga atacacactt tattttaaag acccacatat ttatttacag
    17401 ttagcacatt tatatgtggt tttataacta ttatttacaa gtctatattg ataactttta
    17461 catagtatat aatgtttaaa tacttattca aatttatcta tacataaaca aaaaaagaga
    17521 ttttaaaatc tctttttaga ttgttgactt atggcttttt gatttaataa aatcaagaag
    17581 ccgtttgaat tttatctgtt ggctgatacc cttctgatat tgaaacacca tttttgtttc
    17641 caaaatcgtt taaaattaag aacttattat gaatgactat ttcattatga acaataagca
    17701 gataacatga atcttcaatc aatgttttaa cagtagcatt aaatgcttgc ttatacagtt
    17761 ctatcgtttg tttgaactgt ccattaaagt aaatatgagt caataacata actatatcta
    17821 cacaaattaa tataaatttt ttatactgta cattatctaa ctgttgatta tctgtattac
    17881 aacatgattg aatttaaaaa gccatcaaca catagaatgt tgatggcctg aaaatagatt
    17941 taaaatctct tttttattat atattaatta ttttttaaat ttatattttt tgcatcagat
    18001 tttctaatag ctaagtcaaa ccctctaaat ctaatcacta tagggtcatt aagaggagct
    18061 atctttttca cttcaacttc agtgccatct attagtccta aggctagaag tctcttcata
    18121 agtttttcat ttccagctat attgtctata attccctttt gtccaagttt taagttgtaa
    18181 acagtcatta ttaacacctc ctaatgaaaa tcattatcaa actttatatt tataatatat
    18241 aacaaaaaga gcaaattgtc aatatataac aaaaaataaa catactaagt tagtatgttt
    18301 ataaaaaata gtatacatgg gatactaata gcatctagca aaaaaccact tactaatggt
    18361 acaactaaaa aggcttttgg agaatcacca tatttttgtg tcaaagaact catatttgcc
    18421 agagcgttcg gagtagctcc tattccatgt ccaatcaaac cacttattat gatggcagaa
    18481 tcaaaatctt ttccaagtac tctaaagcaa ataaatattg aaaataggat tataaagatt
    18541 acttgaaaag ttactattaa gagaatagga ggtaataatg tagataattc aaataaatct
    18601 atattcataa gtgataaagt taaaaaaatt cccaatgaaa gttcttttaa aaaatctata
    18661 agtttaaaat ttaattctag tatatttatc ttatcattta aatttctaaa aataacagcc
    18721 gaaaacatac aagttacaac agagggtaat agtatattaa acgttatata gaagcatcta
    18781 cttataattt ctcctgtatt tatacaaata aatatgagaa gaacctgttc caaaaataca
    18841 gatggtgtta ttgtttcgga gaagttatta ttttttatcc tattcttatt tctaaaaaat
    18901 ttacttccaa atctctttag atttctatta tatctaggaa tcgacaagtc tatatttgat
    18961 gatggtttta atttatattt atttattaga aatttagcta caggacatcc caatatacca
    19021 cccataataa gtcctattgt tgcagcagat aatccaaccc caactgctcc ttctataccg
    19081 agattttcta ttgtaagtcc aaatgcagca gaataaccat gaccaccttc catagataca
    19141 tttccacaca taagccctaa aagaggttca agttttataa tctttgatat tgtaatagct
    19201 aatatatttt gacaaaatgc caatactcca cacaaaatcc aatatctaaa taatacacta
    19261 ccacctttct ttataagatt tatgcttatt tcaagtccta tagttgtaaa aaagaaggtt
    19321 atgaaatttg gcattaaatt tccacttata gatatatctg ctatattaaa atatttaaaa
    19381 aataaattta gaaaacaaaa taatagacct cctattactg gtgcaggtat acagaaatta
    19441 tttaaaaagt taactttatt cttcaataaa ttaccaatta aaaagaagaa tatagatagt
    19501 gtcaaggttt gcactatatc aaaacttaag gtaatcaaaa taagtcctcc tttgtaattc
    19561 aaagttttaa ttatataact taaattctta tgtattgaa
//
LOCUS       DAEPIN010000069        19251 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.069, whole genome shotgun sequence.
ACCESSION   DAEPIN010000069 DAEPIN010000000
VERSION     DAEPIN010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19251
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.069"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            324..902
                     /locus_tag="KR250_002905"
     CDS             324..902
                     /locus_tag="KR250_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_126936537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBH0068820.1"
                     /translation="MGITLSKGQKVDLTKGNPGLKNILVGLGWDTNKYDGGFDFDLDT
                     AAFLTGASGSVTNDGDFVFYNNLKHTSGAVEHLGDNRTGEGDGDDEQIVVDLSKIPGE
                     ISKISFTVTIHDATERRQNFGQVSNSYIRIVDKDTNEELIKYELGEDFSIETAIVVAE
                     IYKHNGEWKFNALGSGFEGGLAALCGNFGINL"
     gene            941..1519
                     /locus_tag="KR250_002906"
     CDS             941..1519
                     /locus_tag="KR250_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_126936537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBH0068821.1"
                     /translation="MAIVLKKGQKIDLTKGNPGLKNIKLGLGWDTNSFDSGYDYDLDV
                     SVFMVGESQRVEKDEDFIFYNNLKHPSGAVEHLGDNRTGEGDGDDEEILVNLETMPKH
                     IQKIAVTVTIYEAAERRQNFGQVSNSYIRVLNSENDEEILRYDLGEEFSIETAITVCE
                     IYKYNGEWKFSAVGAGFEGGLEALCRNYGLNV"
     gene            1605..2234
                     /locus_tag="KR250_002907"
     CDS             1605..2234
                     /locus_tag="KR250_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBH0068822.1"
                     /translation="MSINLSKGDKIDLKKSNPGLSNILVGLGWDPVQQSGGGFFKSLF
                     GGGQADIDCDASVFMLNQEGKLSGIKDLIYFGNLKSACKSVLHTGDNLTGEGTGDDEQ
                     ILVNLDKVPSNIHKLLFVVNIYNCVDRKQHFGMIENAYIRVEDQGNKKEIAKYNLSDN
                     YSEKTTLIVGAIYRKDGSWQFKAIGEGTKDAGLKEVMQNLDRIECAYGI"
     gene            2224..4983
                     /locus_tag="KR250_002908"
     CDS             2224..4983
                     /locus_tag="KR250_002908"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HBH0068823.1"
                     /translation="MEFNNDIKYYNVDKEEVTKDLSVNPEKGLSESEVKTRREKYGLN
                     EFTPKEEGSFWDDLKESLSEPMIVILIIAAVVSAVIGETHDAIGIVGAIAIGIAIGMI
                     TEGRSKKAAEALSKLTENIEVKVLRDGEVHQISKSELVPGDIVYIETGDMVPADGRLI
                     ESINLKIREDMLTGESDDVSKKCDVVVSMEEIESKGAVVVQEPIPAKQINMVFGGTLV
                     AYGRGALVVTSIGDNSEMGKIAQNLSETDEQTPLQIKLGNLGGLIAKVSSAVAGLLFI
                     FMVFQMISKNVLNVDMSGILPFLESIDPVKTAFTVCVALIVAAVPEGLPTMINMTLAI
                     TMQKMAKINALVTKKEACETIGSVSVICSDKTGTLTENRMTVEVAYVDGRYIESSEEE
                     INSYFEENCMINSTADVEHNDGDIKYLGSATECALLLYYKNVDYRQVRKNSNIVAQNP
                     FTSDSKRMTSVIGQDNHHVLLSKGAPEVLLELCSHIQRGKDIVPLTENIKHEILEEIK
                     KLQIKSMRTLGFAYKEISQAEEEAAVTAESDAMNVSAMENNLVFSGFVGIRDPLRKDV
                     IESVNTANKAGVSVKMLTGDNINTARAIGEELGLLKNNMRVVEASYIDTLDDEELKQE
                     IQSISIVARSKPDSKMRIVSALQKSGDVVAVTGDGINDAPALSKADVGIAMGISGTEV
                     SKNAADIILTDDSFSTIVKGIKWGRGIYDNFQRFVQFQLTVNVIAFLVAIISQVMGQE
                     MPFTTIQLLWVNIIMDGPPALALGLEPVRDHVLNRKPVDRKANIISKSMVYTIVLNAF
                     YITAILMLQSTFNFLGVDEAHKGTVMFALFAFSALFNAFNCREFNADSIFPNFTHNKL
                     ALQIIVLTGIAQVIFTQLFQDFFNSVAMDFMTWVKVLCVAASVIVVNEVVKCIIRLVK
                     PKKTA"
     gene            5070..6632
                     /locus_tag="KR250_002909"
     CDS             5070..6632
                     /locus_tag="KR250_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceG family protein"
                     /protein_id="HBH0068824.1"
                     /translation="MGDSKVFEKIFSSQSDRGNYTPSKGYLSYFISYIGLEDEVLYNL
                     EIFKTKQNIDSKKDIALFTDVIANPSDFDIINYFKSGLQKYRTSMEDVDINILGFEEI
                     DYKIKQAMDRVLKEEEKEFTNDRVKQNFIVKIMAWIKIYIGALDINKNEAPKVIFYGD
                     IKKHEVYLLLILYLAGFDVLYLNPNSKSNIDILKSERYNIEFEEANIIEEKISFEERV
                     ILGEKIDKSSVKKAFTVGAEASKRISEELLNDAGFIKPWQLQDRKIKNLLLSSTVDEI
                     SIYWNQPLKLRPGFKFNDAIVEAPNFLSKINGIYNDKNEYIKFLDLLRDSESSTFIEF
                     NGDVDRFSKAFTREAFSLSFLLDSKGAIDKNSVLNNKDYSISTLALNQQIMILEKVEE
                     LLEGSMFLNGLSGEDKIKGLFTVLHMDKKFVHMMNNFDYSLINPKLIIYMYKSIVFDK
                     EIVFLMLLLSKIGFDIIILCPGGENNIENVINNQLIDVHRLDKMVYDLKLNSLENDIP
                     LLKKIFGKRRRF"
     gene            6633..7769
                     /locus_tag="KR250_002910"
     CDS             6633..7769
                     /locus_tag="KR250_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HBH0068825.1"
                     /translation="MGININSFSKDKNSVEVTENLPTAVQEENFDIMEYTNNKKNELR
                     KSKEVEALTSLIEVENPDTILQFGRKASEGVARVSDSLLNTIKLNRNEENSKMLVHLT
                     KIMDKFDLDDFQETKEPNFVQKLFKKANNAIEMMFQKYETLGGEVEKIQIELEQYERD
                     IALSNKQIGAMLNENFEFYNELQKYIVAGEMAIEEMDNEILPFFKQKSETSGDQMDVV
                     NYQELLKVYDMLNQRVYDLRIAENIAIQTIPMLRGMQHNNYGLIRKINSAFVVTLPVF
                     KQCLSQAILLKKQELQAKSLKALDDKTNELLLRNAQNVSTQSAQIARMAGTSSVQIET
                     LEKMYNTIKSGIDETMRIEENNRALIKDNTKRLEELNTTIIYNK"
     gene            7833..8909
                     /locus_tag="KR250_002911"
     CDS             7833..8909
                     /locus_tag="KR250_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HBH0068826.1"
                     /translation="MKYFDYICKDDLERIFLKEPEDFSAKTEKDVLKYALGAFLYVPA
                     TQYNMIYKSIIGDVKGVRPLAICLEDAVGVNGELEAIENLRLILKNISNESITNKDGI
                     PLIFVRIKDVEQLLRIKEIIIKNRHSITGILIPKANSELIENCIEALDSMNLQDMYVI
                     PIIETKEFIYNEKKELSFTNLYNAILRHKSRILSIRVGITDILGMYGIRRDKNFSIYN
                     NLICSSFILDIITYLQRPELDIPISGGVSEFFDMTNKDIRNKYIEEILLDKYHGLIGK
                     TVIHPMQIQIVQALSTVSYEDFTDALDILDSTDSKYGVSKGVLGERMNETNPHFLWAK
                     KTLILSKIYGVLNKGVDYEELLKF"
     gene            8887..10125
                     /locus_tag="KR250_002912"
     CDS             8887..10125
                     /locus_tag="KR250_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HBH0068827.1"
                     /translation="MKNCLNFNCEIDIIDNPLNLELNDFLDVGIRNNSKRRFLFISKK
                     LGKHLACKPSEMDNLGKLMATIYNEKNKEESNGTVIAFAETGTAVGHSFFDYLCGDYE
                     FIHTTREQFDELENLEFLEEHSHATDQNLYFGMLNNFKFGDEIILVDDEITTANTCMN
                     IIRKIQSIYPKKKYTICSILNWVSDENLSKVTRLERELDCKIDFVYLFKGNFKFYIDE
                     DIISNYNDNYSITKLETISSDKVNLVVNHIYVDLEDYIGNKKYVKYTGRFGINRREQF
                     RLKEIVKRESKKITMSSKESLKNEKEKILFLGTEEFMYIPMLFAKEKENQYDIYYHST
                     TRSPIINIDKNNYPIKSKFMLKSFYNIDIQNYIYNIDKHNFSKCYLFVELNKSENSYL
                     EFIDIIKKTSIKELTIVCCS"
     gene            10179..11267
                     /locus_tag="KR250_002913"
     CDS             10179..11267
                     /locus_tag="KR250_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease StiP family protein"
                     /protein_id="HBH0068828.1"
                     /translation="MRDFSTYSKDDVIFLLKDISNLIEEEGNKERELKIQNGRHYSEM
                     IPIEYEVSEDYLNLYHEKLRENKDKLAFSIGVMCEKIIKKNGNDVVLVSLARAGTPIG
                     ILAKRYIRSKYNVSLPHYTISIIRDKGIDMNAIEYIVKNHPSSKIQFLDGWTGKGTIS
                     KELEKACDELNVKFKKYFDSTLAVLADPAGYSGLYGIREDFLIPSACLNSTVSGLVSR
                     TVLREDLISKNDFHGAKFYRHLKDKDESMNYIETIEECFKNQFKNISDEVENWESDII
                     TRDGYFDVLNIKEKYNITDINFIKPGVGETTRVLLRRVPYKILVKDLNDKSLDHIFIL
                     AKEKNVEVEQMDLKAYKCCGIIKNMKDI"
     gene            11295..12086
                     /locus_tag="KR250_002914"
     CDS             11295..12086
                     /locus_tag="KR250_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HBH0068829.1"
                     /translation="MIVFSDLDRSIIYSNKFLNADSKYANIEIYREKEISYISLDTIN
                     LIKQIQYYGMFIPTTTRTVEQFKRIEFNKYGIYFPWSITSNGGVILKDNEILKSWSEK
                     IDKLKSDYEPIESMIHKFKDYLNVDGITNFKVAEDTFFYIVVDLSRFNLDSIKEYTNI
                     LESKNWKFYVSGRKIYFIPKEISKENAIKYLTKELGIEYFYAVGDSIMDYGMLNISNK
                     SYVLKHGDINKNEIENSFISSSFNGMSGTEEILSNILDENCLLNI"
     gene            complement(12201..12755)
                     /locus_tag="KR250_002915"
     CDS             complement(12201..12755)
                     /locus_tag="KR250_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HBH0068830.1"
                     /translation="MNIDVGSKIRSLRKSKNISISTLAKNSDLSTGLISQIERNMVVP
                     SIVAMWKISKALDINIGYFFEEIGREDSDIVVKKNNRKKIVTNDSTKFYELLVPNLSG
                     KKIEFILVTLDGHTHKNSEFVTHEGEECGYIIKGKMKITLGNKEYLLEEGDSFYFNST
                     IPHVYENYDDEVCISVWAMTPPSF"
     gene            12951..14153
                     /locus_tag="KR250_002916"
     CDS             12951..14153
                     /locus_tag="KR250_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 domain-containing protein"
                     /protein_id="HBH0068831.1"
                     /translation="MKSFVSPDNIWVLWAVVTGWAAFSIYLEQKYNWASKVSGAIIAL
                     VGAMLLSNLNIIPVESVVYDQVWGYVVPLAIALLLYQCNIKKIWKESGRLLIIFLVGS
                     VGTVLGAMIGFLALKNVVPDLNIVAAMMTGSYIGGNVNFAAMSGAFDAPGELVSATVV
                     ADNLLMALYFFVLIAIPSIGFFRKHFKHPHVDEMESIGITEGETVAANYWGRKEISLK
                     DIAIAIGSAFIIVAVSVELSTWFKAIIPLSNPFLAMLNTLFGNQYLIITTLTMLFATF
                     MPNFFGDIKGAQELGTFLIYIFFVVVGVPASITLIIQKSPLLLLYCGIMVLINMLVTF
                     IVAKIFKFSLEEAILASNANIGGPTTAAAMAISKGWSKLVGPILIVGTFGYIVGNYFG
                     LLVGNILI"
     gene            14210..15190
                     /locus_tag="KR250_002917"
     CDS             14210..15190
                     /locus_tag="KR250_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HBH0068832.1"
                     /translation="MIFDGHSDTWADVTIRRLNGEKDVFRRIHLDNFKRANIEGAIFV
                     IWIDPPYDKEPEKRVKQIISSIKDEIEDMKDVVNIVKKYSDIDKGISENKINILIGLE
                     GLSHIGRNIDLLDYFYKEVGARHASLTWNEQNELATGCLGDENRGLTEIGKKAVNKME
                     KLGMIVDVSHLNEKSFWDLMSVASKPVIASHSNARSLCDAKRNLTDNQLKEISNTGGL
                     VGINSFRGFTNSNPKKQDLIHLVDHIDYMVNLIGIEHVSFGFDFSDYLGKETIGSFTG
                     NASVPSVDGLESTHTAQNIVVELENRGYKKKDIELISSGNFYRLFKDILK"
     gene            15438..16391
                     /locus_tag="KR250_002918"
     CDS             15438..16391
                     /locus_tag="KR250_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HBH0068833.1"
                     /translation="MKKRYLVIGIIILSYFSLFIGAEDINIMHIFAKNQHKLMIFIMS
                     RIPRLISILIAGVGMSIAGLIMQQISKNKFVSPTTGATIDAAQFGIVICMLLVPTASI
                     FTKTIIAFVFSLVGTFTFMKIIGKLQFKNIIFVPLVGIMFGNIIGSMTDFIAYKYDLS
                     QNVSSWMQGDFSMILKGNYEILYITIPLIILAYIYANKFTVVGMGMDFATNLGLSYKR
                     IVNIGLIIVALVTVCVVVTAGNIPFIGLIVPNIVSLYMGDNIRASIWYTGLLGAIFVL
                     ICDIFGRIIIYPYEISIGLTVGVIGSILFLYLILRRNVNEA"
     gene            16381..17337
                     /locus_tag="KR250_002919"
     CDS             16381..17337
                     /locus_tag="KR250_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HBH0068834.1"
                     /translation="MKLSLNQKIYFVSILLFISIALFIIYGIDMNHLEYALSQRIPKI
                     FAMVLGGGCIAFTTVVFQTITNNQILTPSVLGLDSLYVMIQTIIVFIFGSSSSLIINE
                     NYNFIINVAMMIGASLLLYKTLFEKNKNNIFFLILVGMIFGTLFKSATTFIQVMIDPN
                     EFLALQTSIMASLNNINTNVLLIAFIIIIAIIPFIYDEIKYLDVLSLGKEQAINLGVD
                     FDKAVKKMIILIAILVSISTALIGPMTFLGLLLANITREIFKTYKHTYLISGSMLIGM
                     ITLIIGQFFIQHVFKFDTTLSVVINFIGGIYFIQLLLKGANR"
     gene            17334..18089
                     /locus_tag="KR250_002920"
     CDS             17334..18089
                     /locus_tag="KR250_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBH0068835.1"
                     /translation="MIEIKNIFKRYKNKNVVDDVSFSIEKGKITSFIGPNGAGKSTVL
                     SIVTRLIGGDGGEVIIEGKSLTNYSNKELAKKIAILKQSNNITLKLTIRELVGFGRFP
                     YSEGNLTKEDENYIDEAIEYMKLTDIQHKYLDELSGGQRQRAYIAMVIAQDTEYILLD
                     EPLNNLDMNHSVQMMKVLRSLCDELDKTIVLVMHDINFASCYSDNIVALKNGKVKKVG
                     RTDEIVNEKVLEDIYEMNFNIKNINGNRICIYF"
     gene            18181..19146
                     /locus_tag="KR250_002921"
     CDS             18181..19146
                     /locus_tag="KR250_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HBH0068836.1"
                     /translation="MNKKAAIVAAVAIIGLVTVFALGGSKKNESKTSENSNNTMKITH
                     NLGETDVKLNPKKVVVFDYSALDTMDALGVAENLVGLPKASLPASLEKYKDDKYADLG
                     GLKEPDLEGIKSANPDLIIINGRQEDFYEQLSKIAPTISTSKDDKKYLESVKNNIDKI
                     AKIFGVEEKANQEFSKIEKKIETLNKKVTDKNLNALTIMVNEGNLSVFGEESRFSILY
                     NSFGFENKDKNIKESSHGQNITFEYIAKQNPEVMFVIDRGIATGSDVKESSTAKSVLN
                     NDIIKSMDAYKNDNIIYLDSPTWYVNDGGLTSLNKMIDDASKAVN"
BASE COUNT         7636 a         1952 c         3418 g         6245 t
ORIGIN      
        1 ttctcctata ataagatgat ataatctatt ataaaacaac ttaaaacaat atagtacaat
       61 aattttgtag tttcaaaata aaatatgtac ttgtttatat aaatgatata taattacata
      121 ttaaacatag cttttatgca tttaataaag tatgtaaaat ctatgtaaaa agtaagaaat
      181 ataaacattt ttttaattat attttaaaaa tatcaagcaa gttttattca caaatgaata
      241 aaaaatgtta taatatgatt aaagaaaaag ataaataatt agcaagattt gtttagctaa
      301 aattaacaag ggagcgataa attatgggta taacattatc aaaaggccaa aaggttgatt
      361 taactaaagg aaatccaggt cttaaaaata ttttagtagg tttaggatgg gatactaaca
      421 agtatgatgg tggatttgac tttgatttag atacagcagc atttttaaca ggagcaagtg
      481 gaagtgttac taatgatgga gattttgtat tttacaacaa tttaaaacat acatctggag
      541 cagttgagca tctaggagat aacagaactg gcgaaggtga tggagatgat gaacaaatag
      601 ttgttgattt atctaagatt cctggagaaa taagcaagat ttcttttact gttacaattc
      661 atgatgcaac agaaagaaga caaaattttg gacaagtaag taattcttat ataagaatag
      721 tagataaaga cacaaatgaa gagttaataa agtatgaatt aggagaagat tttagtatag
      781 aaacagctat agtagtagct gaaatatata agcacaatgg agaatggaag tttaatgcac
      841 taggttctgg ctttgaaggt ggtcttgctg cattatgtgg aaactttgga ataaacttat
      901 aatttcaact ataacaataa actaaagggg gatattttcc atggctatag tattaaaaaa
      961 aggacaaaaa atcgatttaa ctaagggaaa tccaggtctt aaaaatataa aacttggact
     1021 aggatgggat acaaattcat ttgatagtgg ctatgattat gatttagatg taagtgtttt
     1081 catggttgga gaatctcaaa gagttgaaaa agatgaagat tttatattct acaataactt
     1141 aaaacatcca tctggagcag ttgaacactt aggtgataat agaactggtg aaggtgatgg
     1201 agatgatgag gaaatattag tgaatttaga gacaatgcca aagcatatac aaaagatagc
     1261 tgtaacagtt acaatatatg aggctgctga aagaagacaa aattttggac aagtaagcaa
     1321 ctcatatata agagtattaa attctgaaaa tgatgaagaa atattaagat atgacttagg
     1381 tgaagaattt agtatagaaa ctgcaattac agtatgtgag atatataagt ataatggaga
     1441 atggaagttt agtgcagttg gagcaggatt tgaaggtggc ttagaagcac tttgtagaaa
     1501 ctatggctta aatgtttaat ttaagcaata taatatatta aatataaata acctatacaa
     1561 ataatgtata ggttatatta tataaaattt tgtggaggtt tagtatgtcg attaatttat
     1621 caaaaggtga taaaatagat ttaaaaaaat ctaacccagg actgagtaac atacttgtag
     1681 gtctaggatg ggaccctgtg caacaatctg gcggaggttt ttttaaatcg ttatttggtg
     1741 gaggacaagc tgatatagat tgtgatgcat ctgtatttat gcttaatcaa gaaggtaaat
     1801 taagtggaat aaaagattta atttattttg gaaatctaaa aagtgcctgt aaatcagttt
     1861 tgcatacagg agacaatcta actggtgagg gtacaggtga tgatgaacaa atcctagtta
     1921 atttggataa agttccatca aatattcata aattattgtt tgtagtaaat atatacaatt
     1981 gtgtagatag aaaacaacac tttggaatga tagaaaatgc ctatataaga gttgaagacc
     2041 aaggtaataa gaaagagata gcaaaataca atctatctga taattattct gaaaaaacta
     2101 ctcttatagt aggagctata tatagaaaag atggaagttg gcaatttaaa gcaattggtg
     2161 aaggaacaaa agatgcgggt cttaaagaag taatgcaaaa tttagacagg attgagtgtg
     2221 catatggaat ttaataatga tatcaaatat tataatgtag ataaagaaga agttacaaaa
     2281 gacttatctg ttaatcctga aaaaggttta tcagaatcag aagtcaagac tagaagagaa
     2341 aaatatggtt tgaatgaatt tactccaaaa gaagagggta gtttttggga tgatttaaag
     2401 gaaagtttaa gtgaaccaat gatagttatc ttgattattg cagcagtagt aagtgcagta
     2461 attggggaaa ctcatgatgc aatagggatt gtaggtgcta tagcaatagg tatagcaata
     2521 ggtatgatta cagaaggtcg ttctaagaaa gcagcagagg ctctttctaa gttgactgaa
     2581 aacatagaag taaaagtatt gagagatggg gaagtacatc aaatttctaa gagtgaattg
     2641 gtaccaggtg atatagtata tattgaaact ggtgacatgg tacctgctga tggtagactt
     2701 atagaaagta taaaccttaa aattagagaa gatatgttaa caggagaatc tgatgacgta
     2761 tctaaaaagt gtgatgtagt agtttctatg gaagagatag aatctaaagg agcagtagta
     2821 gtacaggaac caatacctgc aaaacagata aatatggtgt ttggtggtac acttgtagct
     2881 tatggtagag gagctctagt tgtaacttca attggagata atagtgaaat gggtaaaatt
     2941 gctcaaaact tatcagaaac agatgagcaa actccacttc agataaaact tggaaattta
     3001 ggtggtttga tagccaaagt ttctagtgca gtagcaggat tactatttat atttatggtt
     3061 ttccaaatga taagtaagaa tgtattaaat gtagatatgt ctggaatatt accgttttta
     3121 gaatcaatag acccagttaa aacagcattt acagtatgtg ttgcgttgat agttgcagca
     3181 gtgccagaag gtcttccaac tatgataaat atgacacttg caataacaat gcaaaaaatg
     3241 gctaaaataa atgcattggt taccaaaaaa gaagcttgtg aaacgatagg ttctgtttct
     3301 gtaatatgtt cagataaaac tggtacatta actgaaaata gaatgactgt agaagttgca
     3361 tatgttgatg gcagatatat tgaaagttca gaagaggaaa taaatagtta ttttgaagaa
     3421 aactgtatga taaactctac agctgatgta gagcacaatg atggtgatat taagtattta
     3481 ggaagtgcta ctgaatgtgc attgctttta tattacaaaa acgtagatta tagacaagtt
     3541 agaaaaaatt ctaatatagt agctcaaaat ccttttactt cagattcaaa gagaatgact
     3601 tctgttatag gtcaagataa tcatcatgta ctattatcaa aaggagcacc agaagtctta
     3661 cttgaattat gttcacatat tcaaagagga aaagacatag ttccattaac tgaaaatata
     3721 aaacatgaga ttttagaaga aataaagaaa ttgcaaataa aatcaatgag aactttaggg
     3781 tttgcatata aagaaatctc acaagctgaa gaagaagcag cagtaactgc tgaaagtgat
     3841 gctatgaatg taagtgcaat ggaaaacaat ttagtattta gtggttttgt tggtataaga
     3901 gacccactta gaaaagatgt tatagagtct gtaaatacag caaataaagc tggtgtttct
     3961 gtaaaaatgc ttacaggaga taatataaat acggcaagag ctataggtga agagttaggc
     4021 ctattaaaaa ataatatgag agtggttgaa gcttcatata tagatacttt agatgatgaa
     4081 gaattaaaac aagagataca aagtatttca atcgtggcaa gaagtaaacc agatagtaaa
     4141 atgagaatag tttcagctct gcaaaagagc ggagatgttg ttgcagtaac aggtgatggt
     4201 ataaatgatg ctccagcact ttcaaaagca gatgttggta tagcaatggg tatatctggt
     4261 acagaggtat ctaaaaacgc agcagacata attttaactg atgatagttt tagtaccatt
     4321 gtaaaaggta ttaaatgggg tagaggaatt tatgataact tccaaagatt cgttcaattc
     4381 caattaactg taaatgtaat tgcattttta gtggctataa tatctcaagt tatgggacaa
     4441 gagatgccat ttactacaat acaattatta tgggtaaaca tcattatgga tggaccacca
     4501 gcactagcat taggtttaga gccagttagg gaccatgtgt taaacagaaa acctgttgat
     4561 agaaaagcca atataatttc taagtcaatg gtgtatacaa tagtattaaa tgcattttat
     4621 ataacagcaa tactaatgtt acaatctaca tttaatttct taggagttga tgaagcgcat
     4681 aagggaacag taatgtttgc gttatttgct tttagtgcac tgtttaatgc atttaactgt
     4741 agagagttta atgcagatag tatcttccct aattttacac ataacaaatt agcactacaa
     4801 ataatagtgt tgacaggaat tgcacaagta atatttactc agctattcca agatttcttt
     4861 aactctgtag caatggattt tatgacatgg gtaaaagttt tatgtgtagc agcaagtgta
     4921 attgtggtta atgaagttgt aaaatgtata ataagactgg taaaaccaaa aaagactgct
     4981 taaaacaaat tttttagaag aaaacagact gactcaaaat atgaattttg ggtcagttct
     5041 gaataaacat ttaaattggg aggatagtta tgggtgactc aaaggtattt gaaaaaatat
     5101 tctccagcca atcagataga ggaaattaca ctccttcaaa gggatatcta agttatttta
     5161 tttcgtatat tggtttagaa gatgaagttt tgtacaatct cgagatattt aaaacaaaac
     5221 aaaatattga tagcaaaaaa gatattgctt tatttactga tgttatagca aatcctagtg
     5281 attttgatat tataaattat tttaaaagtg gacttcaaaa atatagaact agcatggaag
     5341 atgtagatat aaatattttg ggatttgaag agattgatta taaaataaag caagcaatgg
     5401 atagagtgtt aaaagaagag gaaaaagaat ttactaatga tagagttaaa caaaatttta
     5461 tagtgaaaat aatggcatgg ataaaaattt atatcggtgc tttagatata aataaaaatg
     5521 aagctccaaa agtaatattt tatggagata taaaaaaaca tgaagtttat ttactcttaa
     5581 tattatactt ggcaggtttt gatgttttat atcttaatcc aaattcaaag agtaatatag
     5641 atattttaaa atcagaaaga tacaatatag agtttgaaga agccaacata atagaagaaa
     5701 aaatttcttt tgaagaaagg gttattttag gggaaaaaat agataaaagt tctgtaaaaa
     5761 aagcttttac tgttggggca gaagcatcaa agcgtataag tgaggaattg ttaaatgatg
     5821 caggatttat aaaaccttgg caactccaag atagaaaaat aaaaaatcta cttctaagtt
     5881 caactgttga tgaaatatca atatactgga atcaaccact taaattaaga ccaggattta
     5941 agtttaatga tgctattgta gaagctccaa attttctttc taagattaat ggaatatata
     6001 acgataagaa tgaatatatt aagtttttag atttattaag agattcggaa agttcaacat
     6061 ttattgaatt taatggagat gtagatagat tttcaaaagc ttttacaaga gaagctttta
     6121 gtttatcctt tttattggat tcaaaaggtg caatagataa aaactctgtt ttaaataata
     6181 aagattattc catatcaaca ttagcattga atcaacaaat tatgatttta gagaaagtgg
     6241 aagagttgtt agaaggaagt atgtttttga atggtttaag tggagaagac aagataaaag
     6301 gtctatttac agtacttcac atggataaga aatttgttca tatgatgaac aatttcgatt
     6361 actcacttat aaacccaaaa ttgattatat acatgtacaa gagtattgta tttgataaag
     6421 aaatagtttt tcttatgctt ttacttagta agattggatt tgatattatc atattatgtc
     6481 caggaggaga aaacaatata gagaatgtta taaataacca attgattgat gtacacagat
     6541 tggataaaat ggtatatgac cttaaattaa attcgcttga aaatgatata cctttactta
     6601 aaaaaatttt tggaaagagg agacgatttt agatggggat aaatataaat agtttttcaa
     6661 aagataagaa ctctgtagag gttactgaga atttgcctac agcagttcaa gaagaaaact
     6721 tcgatataat ggaatataca aataataaaa aaaatgaact gagaaagtct aaggaagttg
     6781 aggcattgac atctcttata gaagtagaaa atccagatac gattttacag tttggtagga
     6841 aagcatctga aggagtagca agagtttctg atagcctttt aaatacaata aaattaaata
     6901 gaaatgaaga aaattctaaa atgctggttc atttaactaa aataatggac aagtttgatt
     6961 tagacgattt tcaagaaact aaggaaccaa atttcgttca aaaacttttc aaaaaagcta
     7021 ataatgccat tgaaatgatg tttcaaaagt atgaaacatt gggaggagaa gttgagaaaa
     7081 ttcaaattga gcttgaacaa tacgaaagag atatagcact ttctaataag caaataggtg
     7141 ctatgttaaa tgaaaacttt gagttttaca atgaacttca aaaatatatt gttgctggag
     7201 aaatggcaat agaagaaatg gataatgaaa tactaccttt ctttaaacaa aaatcagaga
     7261 ctagtggaga ccaaatggat gttgtcaatt atcaagaact attaaaagtt tatgatatgt
     7321 taaatcaaag agtctatgat ttaaggatag cagaaaatat agctatccaa acaataccta
     7381 tgcttagagg tatgcaacat aataattatg gtttaattag aaaaataaat agtgcatttg
     7441 tcgtaacttt accagtattt aaacaatgtt tatctcaagc tatattactt aaaaagcaag
     7501 aattacaagc aaaatctcta aaagcacttg atgacaaaac taatgaatta ttgcttagaa
     7561 atgcacaaaa tgtaagtact caaagtgcac aaatagctag aatggctgga acaagttctg
     7621 ttcaaataga aacattggaa aaaatgtaca atactattaa atctggtata gatgaaacta
     7681 tgagaataga agaaaacaat agagctttaa ttaaggataa tacaaagaga ttggaagaat
     7741 taaatacgac tataatttat aataagtagt attatattaa gggctaattt gtataaagtt
     7801 ggccctttct atgtatatga ggaggctaga cattgaaata tttcgattat atctgcaaag
     7861 atgatttgga gagaattttt ttgaaggagc cagaagattt tagtgctaag actgaaaaag
     7921 atgtgttgaa atatgcactg ggcgcttttt tatacgttcc agctactcaa tataatatga
     7981 tttataagag tattataggg gatgttaagg gagttagacc attagctatt tgtttagaag
     8041 atgctgttgg agtaaatgga gagttagagg ctatagaaaa tcttagatta attttaaaaa
     8101 atattagcaa tgaaagtata actaataaag atggaatacc actaattttt gtaagaataa
     8161 aagatgtgga gcaactgttg agaattaaag agattataat taaaaataga cattctataa
     8221 caggaatatt aattcctaaa gcaaattcgg agttaataga aaattgtata gaagctttag
     8281 attctatgaa tcttcaagat atgtatgtta ttccgataat agagacaaaa gaatttatat
     8341 ataatgaaaa aaaagaactt agttttacta atttgtacaa tgctatttta agacataagt
     8401 caagaatatt aagcattaga gtaggcataa ctgatatatt agggatgtat ggtataagga
     8461 gagataagaa cttctctata tataataatt tgatttgttc ttcatttata ttagatataa
     8521 tcacatatct tcaaagacca gagttagata tacctataag tggtggagta tcagaatttt
     8581 ttgatatgac gaataaggat ataagaaata agtatataga agaaatatta ttggataaat
     8641 atcatggatt aattggaaag accgtaattc atccaatgca aattcaaatt gtacaagcgc
     8701 tatctacagt atcatatgaa gattttactg atgctttgga tatactggat agtacagaca
     8761 gtaagtatgg tgtgagcaag ggtgtattag gagagagaat gaatgaaaca aatccacatt
     8821 ttttatgggc taaaaaaaca ttgattctat ctaaaatata tggtgtatta aataaaggag
     8881 tagactatga agaattgctt aaattttaat tgtgagattg acattattga taatccattg
     8941 aatttagaac taaatgattt tttagacgta ggaataagaa ataatagcaa aagacgtttt
     9001 ttgtttatct caaagaaact cggtaaacat ttagcatgta agccaagtga gatggataat
     9061 ttgggaaaat taatggctac aatttataat gaaaaaaata aggaagaatc aaatggtact
     9121 gttatagctt ttgctgaaac tggtacagct gttgggcata gtttttttga ttacttatgt
     9181 ggagattatg aatttattca tactactaga gagcagtttg acgagttaga aaatctggaa
     9241 tttttagagg aacattccca tgcaacagac cagaatttat actttggtat gcttaacaac
     9301 tttaaatttg gagatgagat aatattggtt gatgatgaga ttaccacagc caatacatgc
     9361 atgaatataa ttaggaaaat tcaaagcatt tatcctaaaa agaaatacac tatatgctca
     9421 atactcaatt gggtaagtga tgaaaattta agtaaagtta ctcgtttaga aagagagcta
     9481 gattgtaaga tagattttgt atacttattt aaaggaaact ttaaatttta tatagatgaa
     9541 gatattattt caaattacaa cgataattat agtattacta aattagagac tatttcaagt
     9601 gataaagtaa atttagtagt aaatcatatt tatgttgatt tagaagatta tattggaaat
     9661 aagaaatatg taaaatatac aggaagattt ggaattaata gaagagaaca atttagatta
     9721 aaagaaatcg ttaagagaga aagtaaaaaa ataactatgt ccagtaaaga atctttaaaa
     9781 aatgaaaaag aaaagattct atttttaggt actgaagaat ttatgtatat acctatgctg
     9841 tttgcaaaag aaaaagaaaa tcaatatgat atatactatc attcaacaac aagaagtcca
     9901 attattaata ttgataagaa taattatcct attaaaagta aatttatgct aaagagtttt
     9961 tataatatag atattcaaaa ttatatttac aatatagata aacataattt ttctaagtgt
    10021 tatttatttg tagagctgaa taaaagtgaa aattcttatc tagaatttat tgatataatt
    10081 aaaaaaacaa gtataaaaga attaactata gtatgttgta gctaaagtgt tttaacagga
    10141 taaaatatat cacgatttta agataggagt tgagtcatat gagagatttt agcacgtatt
    10201 ctaaagatga tgttattttt ttattaaaag atatatcaaa tttaattgaa gaagaaggaa
    10261 ataaagaaag agaattaaaa atacaaaatg gtagacatta ttcagaaatg ataccaatag
    10321 agtatgaagt tagtgaagat tatctgaatt tatatcatga gaaactaaga gaaaataaag
    10381 ataaacttgc attttctata ggagttatgt gtgaaaaaat aattaaaaaa aatggaaatg
    10441 atgtagtact agtttcactt gctagagcag gaacacctat tggtattttg gcaaaaagat
    10501 atataagaag caagtataat gtaagtttac ctcactatac aatctctata ataagagata
    10561 agggaataga tatgaatgca attgaatata tagtaaagaa tcatccgagc tcaaaaattc
    10621 aatttttaga tggatggact ggtaaaggaa ctatatcaaa agaacttgaa aaagcatgtg
    10681 atgaacttaa tgttaaattt aaaaaatatt ttgactcaac acttgctgta ttagctgacc
    10741 ctgctggata ttcaggactt tatggaatta gagaagattt cttaatacca agtgcatgtt
    10801 taaattcaac agtttctggt ttagtaagta gaactgtact aagagaggat ttaattagta
    10861 aaaatgactt tcatggagct aagttttata gacatttaaa agataaagat gaatctatga
    10921 actatatcga aactatagaa gaatgtttta aaaatcaatt taagaatata tctgatgaag
    10981 ttgaaaactg ggaaagtgac attataacaa gagatggata ctttgatgtt ttaaatataa
    11041 aagaaaagta taacataaca gatattaact ttatcaaacc aggtgtaggg gaaacgacta
    11101 gggttttact tagaagagtt ccgtataaga ttcttgttaa agacttaaat gataaatctt
    11161 tagaccatat attcatatta gctaaggaga aaaatgttga agttgaacaa atggatttaa
    11221 aagcctataa atgttgtgga ataataaaaa atatgaaaga tatttaggaa atatattaga
    11281 caggagaata ttgaatgatt gttttttcag atttagacag gagtatcatt tactccaata
    11341 aatttttaaa tgctgatagt aaatatgcta atatagaaat ttatagggaa aaagaaatat
    11401 cttatatatc tttagatact ataaatttaa taaagcaaat acaatattat ggtatgttta
    11461 tacctacaac tactagaaca gtggagcaat ttaagcgtat agaatttaat aaatatggga
    11521 tttattttcc ttggagtata acttcaaatg gaggagttat acttaaagat aatgaaatat
    11581 tgaaatcatg gagtgaaaaa atagataaac taaaatctga ttatgagccc attgaaagta
    11641 tgattcataa atttaaagac tatttgaatg tagatggaat aactaatttt aaagttgcag
    11701 aagatacatt tttttatata gttgttgatt tatcaagatt taatttggat tcaatcaaag
    11761 aatatacaaa tatattggaa tcaaaaaatt ggaagtttta tgtgagtggc agaaaaatat
    11821 attttatacc aaaagaaatt tcaaaagaaa atgctataaa atacttgact aaagaattag
    11881 gaatagaata tttttatgca gttggagatt ctattatgga ctatggtatg cttaatatct
    11941 caaataaatc ctatgtattg aagcatggtg atataaataa aaatgaaata gaaaattcat
    12001 ttatatcaag ttctttcaat ggaatgagtg gaactgaaga gatactatct aacattttag
    12061 acgagaactg cctacttaat atataatgac tgatatataa ataataaact agaaaaatct
    12121 gaaataaatt atagtaaagt ttatcatact aatttatttc agatttttat caatttatat
    12181 ttaagtaaga actcaatatg ttaaaatgaa ggtggagtca ttgcccagac tgagatacat
    12241 acctcatcat cataattttc atacacatgt ggtatagtac tgttaaaata aaaactatct
    12301 ccttcctcca atagatattc cttatttcca agtgttatct tcattttacc ttttattata
    12361 tagccacatt cttctccttc gtgtgtcaca aattcactgt ttttgtgtgt atgaccatct
    12421 agtgtaacaa gtataaattc tatttttttc ccagataaat taggaacaag aagttcgtaa
    12481 aattttgtag aatcattagt tactattttt tttcgattat ttttctttac tactatatca
    12541 gagtcttctc ttccaatctc ttcaaaaaaa taacctatat ttatatcaag agcttttgat
    12601 attttccaca ttgctacaat agatggaaca accatattac gctcaatttg acttattaag
    12661 cctgtactta aatctgaatt ttttgctaaa gtagagatac ttatattttt agattttcta
    12721 agagatctaa ttttgcttcc aacatctata ttcattagat acaaaccccc tcagacatga
    12781 ttataacata taatttataa agattaataa aataaaaaaa aagttttaca atacaagtat
    12841 tttttatgct atattgaatt ttctgaaaag atgcattata atagtattaa tattcaatat
    12901 attgaatatt gataacgata ttttaattta atgaaatgga ggtaattaag atgaagagtt
    12961 ttgtaagtcc tgataatatt tgggtattat gggcagttgt aactggatgg gcagcattta
    13021 gtatttattt ggaacaaaag tacaattggg catcaaaagt gtcgggagca ataatagctc
    13081 ttgtaggagc aatgttatta tcaaatctaa atattatacc agtagagtct gttgtatatg
    13141 accaagtatg gggatatgtt gttcctttag ccatagcttt attgttatat caatgtaaca
    13201 ttaaaaagat atggaaagaa agtggaagat tgctcattat atttcttgta ggttctgtag
    13261 gaactgtact tggagctatg attggatttt tagcattaaa aaatgtagta ccagatttaa
    13321 atattgttgc agctatgatg acaggttctt atataggtgg aaatgtaaac tttgctgcta
    13381 tgtcaggggc gtttgatgcg ccaggagaat tagtatcagc aacagttgta gcagataatc
    13441 ttttaatggc attatatttc tttgtcttaa tagctatacc atcaattgga ttttttagaa
    13501 aacattttaa acatccacat gtagatgaaa tggaaagtat aggtataact gaaggagaaa
    13561 cagtagcagc aaattattgg ggaagaaaag aaatatcttt aaaagatata gcaatagcca
    13621 ttggtagcgc ttttataatt gtcgcagtat ctgtagaact atcaacttgg tttaaagcta
    13681 taataccatt atcaaatcct tttttagcaa tgttaaatac tttatttgga aatcaatatc
    13741 taataataac tactttgact atgttgtttg ccacatttat gcctaatttc tttggtgata
    13801 taaaaggagc acaggaattg ggtacatttt taatatatat attctttgtt gtagtaggtg
    13861 ttcctgcttc aataacatta ataattcaaa aatcaccatt actactttta tactgcggaa
    13921 taatggtact aataaacatg ctagttacat ttatagttgc aaaaatattt aagtttagtt
    13981 tagaagaagc tattcttgca tctaatgcaa atattggtgg tcctacaaca gcagcagcta
    14041 tggcaatatc aaaaggatgg tctaagctag tagggccaat acttatagtt ggaacatttg
    14101 gatacatagt cggtaattat tttggtcttt tagtaggaaa tattttgata taattttacg
    14161 tgaaaaaaag ttgtttttta gaaagatttt tatttaattg gaggttgtta tgattttcga
    14221 tggacattca gatacttggg cagatgtaac aataagaagg cttaacgggg aaaaagatgt
    14281 atttagaaga atccatcttg ataattttaa aagagcaaat atagaaggag ctatttttgt
    14341 aatatggata gacccaccat atgataaaga gcctgaaaaa agagttaaac aaattataag
    14401 ctctataaaa gatgaaattg aagatatgaa agatgttgta aacatagtaa aaaaatattc
    14461 agatatagat aaaggtataa gtgaaaacaa aataaatatt ttaattggtc ttgaaggatt
    14521 aagccatatt ggaagaaata tagatttact agattatttt tataaagaag ttggtgcaag
    14581 acatgcatca ttaacttgga atgaacagaa tgaacttgct acaggatgtt taggggatga
    14641 aaatcgtggt ttaactgaaa taggtaaaaa agcagtcaat aaaatggaaa aattgggcat
    14701 gatagtagat gtgtcacatt taaatgaaaa atcattttgg gatttaatgt ctgttgcaag
    14761 taagccagtt atagcttctc attctaatgc taggagttta tgtgatgcta aaagaaatct
    14821 tacagataat caattaaaag aaatttcaaa tacaggtgga ttagttggaa taaatagttt
    14881 tagaggattt actaatagta atcctaagaa gcaagattta atacatttgg ttgaccatat
    14941 agactatatg gtaaatctta taggtataga acatgtatct tttggttttg attttagtga
    15001 ttacttaggg aaagagacta taggttcttt tactggaaat gctagtgtgc caagtgtaga
    15061 tggtcttgaa agcacacata cagcacagaa catagtagta gagcttgaaa atagaggtta
    15121 taaaaagaaa gatatagagt taatctctag tggtaatttt tataggctct ttaaagatat
    15181 actaaaataa tataataaag aacttaaatt tataaaatag tatatttata caaataatat
    15241 ctacatattt aaattggcgt tcataattta ttaaattaaa ttttaatgtt actagaagtt
    15301 tcaattctag taacattttt tatgttaaaa acagatatag atatatattg acaatgataa
    15361 ttattattga taaactataa aagtgaaaat gataataatt ttcattttaa ttaataaaag
    15421 atttttaagg agggagaatg aagaaaaggt atttagtcat aggaataata attttgtctt
    15481 atttttcttt atttatagga gctgaagata taaatataat gcatatattt gccaaaaatc
    15541 agcataaact tatgatattt atcatgagta gaataccaag attaataagt atattgattg
    15601 ctggtgtagg aatgagcata gcaggtctta taatgcaaca gataagtaag aataaatttg
    15661 tatcacctac aacaggtgct acaatagatg cagcacaatt tgggatagta atatgtatgc
    15721 ttttagtacc aactgcctct atttttacaa agacaataat agcatttgta ttttctcttg
    15781 tagggacttt tacgtttatg aaaataatag ggaagttaca atttaaaaat ataatctttg
    15841 taccattagt aggtataatg tttggaaata taataggttc tatgacggat tttatcgcgt
    15901 ataaatacga tttgagtcaa aatgttagtt cttggatgca aggtgacttt tctatgatac
    15961 ttaaaggaaa ttatgagata ttatatatca caatacccct tataatatta gcatatattt
    16021 atgcaaataa atttactgtt gttggtatgg gtatggattt tgcgacaaac ttaggtctta
    16081 gttacaaaag aattgtcaat ataggtctga taatagtagc acttgttaca gtatgtgtag
    16141 tcgttacagc tggtaatatc ccttttatag gtctaatagt tcccaatatc gtatccttgt
    16201 atatgggtga taatataaga gcaagcatat ggtatactgg tttacttgga gcgatatttg
    16261 tacttatatg tgatattttt ggaagaatta ttatttatcc atatgaaata tctatagggc
    16321 ttacagttgg agttatagga agtatactat tcctttattt aatattaagg aggaatgtaa
    16381 atgaagctta gtctcaatca aaaaatttac tttgtaagta tattactatt catatcaata
    16441 gcacttttta taatttatgg aatagacatg aatcatttag aatatgcatt atcacaaaga
    16501 ataccaaaaa tatttgctat ggtattaggt ggaggatgta tagcatttac tactgtagtg
    16561 tttcaaacaa ttacaaacaa tcaaatatta acaccaagtg tactaggttt agattcattg
    16621 tatgtaatga ttcaaactat aatagtattc atatttggtt caagcagtag tctgattata
    16681 aatgaaaatt ataattttat tattaatgta gctatgatga ttggagcttc attgttatta
    16741 tataagacgt tatttgaaaa aaataaaaat aatattttct ttttaatcct agtaggaatg
    16801 atatttggaa ccttatttaa aagtgcaaca acatttattc aggtaatgat agaccctaat
    16861 gaatttttag ctttacaaac tagtataatg gcaagtttaa acaatataaa cactaatgta
    16921 ttgttaatag catttataat aattattgcc ataatacctt ttatatatga tgaaataaag
    16981 tatttagatg tattgtcgtt aggtaaagaa caagcaatta atttaggtgt agattttgat
    17041 aaagcagtta aaaaaatgat aatattaata gcaattttag tctcaatatc aacagcactt
    17101 attggaccta tgacattttt aggactttta cttgctaata taacaagaga aatttttaaa
    17161 acatataagc atacatactt gatttcgggt tctatgttga tagggatgat tacattgata
    17221 ataggtcaat tttttataca acatgtattt aagtttgata caactttaag tgtagtaata
    17281 aattttatag gaggaattta ctttattcaa ttactattaa agggggcaaa tagatgatag
    17341 agataaaaaa tatatttaaa agatataaga ataaaaatgt tgtagatgat gtctctttta
    17401 gtatagaaaa gggaaagata acatctttta taggaccaaa tggagctgga aaaagtacag
    17461 tactatcaat agttacaaga cttataggag gtgatggtgg agaagtaata atagaaggaa
    17521 aaagcttgac taattatagc aataaagaat tagcaaaaaa aatagctatt cttaaacaat
    17581 ctaacaatat aactttaaag cttacaataa gagaattagt tggatttgga agatttccat
    17641 atagtgaagg taatctaact aaagaggatg aaaattatat tgatgaagca atagaatata
    17701 tgaagttgac tgatatacaa cataagtatt tagatgaatt gagtggaggt caaagacaaa
    17761 gagcttatat agcaatggta atagctcaag atacagagta catattactt gatgaaccac
    17821 tgaacaattt ggatatgaac cattccgtac agatgatgaa ggtacttaga agtctttgtg
    17881 atgagttaga taagactata gtattagtaa tgcatgatat aaattttgca tcatgttatt
    17941 cagataatat agttgcatta aaaaatggaa aagttaaaaa agtaggtcgt acagatgaga
    18001 tagtaaatga aaaagtttta gaagatattt atgagatgaa ttttaacatt aagaatataa
    18061 atggaaatcg aatctgtatt tatttttaaa atattgtata tgaaaataaa cttgctatat
    18121 gattttttat aaagttttta aattaaaata aagattaata ggagagagga aaatattgat
    18181 atgaataaaa aagcagcaat agtagcagca gtagctatta taggattagt gacagtgttt
    18241 gcattaggtg gaagtaaaaa gaatgaatca aaaacatcag aaaattcaaa taatactatg
    18301 aagataactc ataacttggg agaaacagat gtaaaattaa atcctaaaaa agtagtggta
    18361 tttgattatt cggctttaga tacaatggat gcattaggtg tagctgagaa tttagtagga
    18421 cttccaaaag cgagtttacc agcatcctta gaaaaatata aggatgacaa gtatgcagat
    18481 ttaggaggat taaaagaacc agatttagag ggtataaagt cggcaaatcc agatttaatt
    18541 ataataaatg gaagacaaga agatttttat gagcaattgt caaaaatagc accaacaata
    18601 agtacaagta aagatgataa gaagtactta gaatctgtta aaaataatat agacaaaata
    18661 gcaaaaatat ttggagtaga agaaaaagca aatcaagaat ttagtaaaat tgaaaagaaa
    18721 atagaaactt tgaataaaaa agtaacagat aaaaatttaa atgctttaac tataatggta
    18781 aatgaaggta atttaagtgt atttggtgaa gaatcaagat ttagtatatt atacaatagt
    18841 tttggatttg aaaataaaga taaaaatata aaagaatcaa gtcatggaca aaatattact
    18901 tttgaatata tagctaaaca aaatccagaa gttatgttcg ttatagatag aggaatagct
    18961 actggcagtg atgtaaaaga aagctcaact gcaaaatctg ttttgaacaa tgatataata
    19021 aaatctatgg atgcatataa gaatgataat ataatatatt tagattcacc aacatggtat
    19081 gtaaatgatg gtggtctaac atctttaaat aaaatgatag atgatgcttc aaaagcggta
    19141 aattaataat ccgatataat caaaatatat tttttataaa atgattatca aataaactag
    19201 gagtttttaa gaactcctag tttatttttt gcataaaaaa gatatagttt a
//
LOCUS       DAEPIN010000070        19158 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.070, whole genome shotgun sequence.
ACCESSION   DAEPIN010000070 DAEPIN010000000
VERSION     DAEPIN010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19158
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.070"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            27..101
                     /locus_tag="KR250_002922"
     tRNA            27..101
                     /locus_tag="KR250_002922"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:59..61,aa:Thr,seq:tgt)
     gene            116..200
                     /locus_tag="KR250_002923"
     tRNA            116..200
                     /locus_tag="KR250_002923"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:150..152,aa:Tyr,seq:gta)
     gene            210..293
                     /locus_tag="KR250_002924"
     tRNA            210..293
                     /locus_tag="KR250_002924"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:244..246,aa:Leu,seq:tag)
     gene            317..391
                     /locus_tag="KR250_002925"
     tRNA            317..391
                     /locus_tag="KR250_002925"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:349..351,aa:Gly,seq:gcc)
     gene            416..492
                     /locus_tag="KR250_002926"
     tRNA            416..492
                     /locus_tag="KR250_002926"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:450..452,aa:Arg,seq:tct)
     gene            502..577
                     /locus_tag="KR250_002927"
     tRNA            502..577
                     /locus_tag="KR250_002927"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:535..537,aa:Gln,seq:ttg)
     gene            586..661
                     /locus_tag="KR250_002928"
     tRNA            586..661
                     /locus_tag="KR250_002928"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:619..621,aa:Lys,seq:ttt)
     gene            664..752
                     /locus_tag="KR250_002929"
     tRNA            664..752
                     /locus_tag="KR250_002929"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:698..700,aa:Ser,seq:tga)
     gene            756..831
                     /locus_tag="KR250_002930"
     tRNA            756..831
                     /locus_tag="KR250_002930"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:789..791,aa:Phe,seq:gaa)
     gene            838..911
                     /locus_tag="KR250_002931"
     tRNA            838..911
                     /locus_tag="KR250_002931"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:872..874,aa:Met,seq:cat)
     gene            926..1002
                     /locus_tag="KR250_002932"
     tRNA            926..1002
                     /locus_tag="KR250_002932"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:960..962,aa:Ile,seq:cat)
     gene            1020..1096
                     /locus_tag="KR250_002933"
     tRNA            1020..1096
                     /locus_tag="KR250_002933"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1054..1056,aa:His,seq:gtg)
     gene            1105..1180
                     /locus_tag="KR250_002934"
     tRNA            1105..1180
                     /locus_tag="KR250_002934"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1138..1140,aa:Lys,seq:ttt)
     gene            1188..1261
                     /locus_tag="KR250_002935"
     tRNA            1188..1261
                     /locus_tag="KR250_002935"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1220..1222,aa:Cys,seq:gca)
     gene            1269..1345
                     /locus_tag="KR250_002936"
     tRNA            1269..1345
                     /locus_tag="KR250_002936"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1303..1305,aa:Arg,seq:acg)
     gene            1358..1433
                     /locus_tag="KR250_002937"
     tRNA            1358..1433
                     /locus_tag="KR250_002937"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1391..1393,aa:Val,seq:tac)
     gene            1509..1922
                     /locus_tag="KR250_002938"
     CDS             1509..1922
                     /locus_tag="KR250_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0068837.1"
                     /translation="MYIVIGNEIVDSEELKIIIDKNSEFKVEKDLSKSTKREDVIAYQ
                     LSIDLNYLDSLINEQCNLASLSDEEKFDEYMTLSDELALDLEELMPKYTIINARAYKL
                     DEVDGIVKIILAVAYADLGHLKLSDVVKRLSRQVD"
     gene            2231..5662
                     /locus_tag="KR250_002939"
     CDS             2231..5662
                     /locus_tag="KR250_002939"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HBH0068838.1"
                     /translation="MLKKFNKILVANRGEIAIRIFRACSELGIKSVGIYSKEDKYGLF
                     RTKADESYLIGEGKGPIDAYLDMDGIIDLAKRKKVDAIHPGYGFLAENAEFARKCEEN
                     GITFIGPSSKVMNMMGDKINSKKIAKEVNVQTIPGVEKAIRSTEEAKEVANKIGYPVM
                     IKASNGGGGRGMRIVHREEDLELEYETACSESRKAFGEDIIFIEKYIADPKHIEVQIL
                     GDNYGNIVHLYERDCSVQRRHQKIIEYAPAFSLDDKVRKEICEDAVKLSKHVGYSNAG
                     TLEFLVDANGGHYFIEMNTRVQVEHTVTEMVTGIDIVQSQILIAQGYSLDSEEINIKS
                     QDDVEIRGYSIQCRITTEDPKNKFMPDTGKIQVYRTGSGFGIRLDGGNGFTGANISPH
                     YDSLLVKTISWDRTFQGAINKTIRSIKELRVRGVKTNVGFLVNVLNNPIFSNGKCSTK
                     FIDENPDLFEITESKDRGTKLLQFIGDVIVNDNACKEKPLFDALHDPRMDKDGSKSEG
                     SKILFDKLGKSAYIEKIKNDKKLLLTDTTMRDAHQSLLATRIRTYDLLKAAKPTEKYQ
                     KDLFSLEMWGGATYDVAYRFLKESPWRRLQKLREEIPSIMFQMLLRASNGVGYKNYPD
                     NVIEEFTKESARQGIDVFRIFDSLNWVENMKPSINTALETGKIVEATMCYTGDILDKT
                     KTKYNLEYYIKMAQELESLGADIIAIKDMSGLLKPYSAYTLVKELKKNVKAPIHLHTH
                     DTSGNGVATCLMASEAGVDIIDAALESMAGLTSQPSLNAIVEALKNTERDTGIDLFGY
                     DELGKYYKDLRKVYNKFESDLTNSCAEIYNFEIPGGQYTNLKPQADSLGLVNRFDEVK
                     EKYKEANEVVGDIIKVTPSSKVVGDLAIFMTKNKLDKDNIIEEGKNLSFPDSVVDYCK
                     GMIGQPEGGIPKDLQEVVLKGEEAITVRPGSLLPAEDFDEIAKYLNEKYDINANIRNV
                     ISYALYPKVYEDYIKHLQHYNDISKLESDVFFYGLNKNEECEVEIEEGKVLTIRLVEI
                     GEVKENGFRTIGFELNGMVREVEIKDKNFSGKINNVEKADMNDPLQIGASIPGKVIKI
                     MVKEEDEVKANQPLIVIEAMKMETIIVAKTDGVIKSIKVKEDDMVEDKQLLMIMK"
     gene            5904..7832
                     /locus_tag="KR250_002940"
     CDS             5904..7832
                     /locus_tag="KR250_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HBH0068839.1"
                     /translation="MKVYDSKMLRNVAVLGHSGCGKTNLIETIAYTANTNKIPKLTDK
                     VNMTYSMGLIPIEYNDYKFNLLDTPGYFDFSGDVVSSLRASDAAIIVIDATAPIQVGT
                     EKSLELTESIPKIMFINKIDNEKARYKDAIAMLREKYNNKIVPMISPIYKDKNFVKLH
                     NVFENIDDLEGEFKEQAMSVKEALMELIAETDDQILDKYFNGEELTTEEIQKGIIIGI
                     QRGDIIPVICGSTINNIGTKEILDTISSYLEPIFTEESKPFRGLVFKTMVDPFVGKMS
                     YIKITEGVLSKDKDVFNINKNVKEKIANIYTLRNSELVEIEKAKAGDIVVITKVNSLK
                     TGDTISADKDAEALEKIDFPKPQIYYAVTPKNKGDEEKVASVLNKLVEEDPTLHWYRN
                     TETKQALLGGQGELHIKTIKNKMKDKFGVDVELNDLKVPYRETIKGTADVQGKHKKQS
                     GGHGQYGDVKIRFERCESDFEFTEEIFGGSVPKQYIPAVEKGLKDSMQKGILAGYPVT
                     NIKATLYDGSYHDVDSSEMAFKMAASAAFKKGMEEAHPILLEPIMKLKITVPEEYMGD
                     VMGDINKRRGKIFGMEPDDKGKQIIFAEAPQAETFKYAIDLRAMTQGRGYFEMELERY
                     GEVPSQFAEKIIGLATAK"
     gene            8285..8749
                     /locus_tag="KR250_002941"
     CDS             8285..8749
                     /locus_tag="KR250_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HBH0068840.1"
                     /translation="MATMTDIIEKFIKDLMEEDNSIQIQRNELANLFSCAPSQINYVL
                     TTRFTIDRGYYIESKKGGGGYVQIEKIRKSKDGHIRELLNEKIGSQISYKKAKELLEG
                     LKESDLINERELKLILYAIDDKSLCMPIYELKEKVRSNILKNIIIGLFSIEE"
     gene            8756..9250
                     /locus_tag="KR250_002942"
     CDS             8756..9250
                     /locus_tag="KR250_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HBH0068841.1"
                     /translation="MLCQKCNKNKASVYYNKIVNGEKTEMYLCSECAKENTEMNFNLD
                     MPFSMMDIFSNLGFQPKKELEEKLVCPKCNTTYSEFKNNGRFGCSECYNAFSSQVNPM
                     LQNIHGHIEHTGKAPKKSFYKISVENEIKELKEDLDRAIKNEEYELAAQFRDKIKYLK
                     GSID"
     gene            9266..10291
                     /locus_tag="KR250_002943"
     CDS             9266..10291
                     /locus_tag="KR250_002943"
                     /EC_number="2.7.14.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HBH0068842.1"
                     /translation="MKENIVMKSRVRLARNLNNYPFPNKLDKECAMEIIEKVKNAFIN
                     SNLEQKEEFDFYKIEDLDQSKKMLMVEEHIISPDLAENDKSAVIVKKDKTISIMINEE
                     DHIRIQTICDDLNLEYAYSVANEIDDLLESSLEYAFNTKLGYLTSCPTNTGTGMRASV
                     MMHLPALSQLGYMDELYKISSQIGIAIRGIYGERTEALGNIYQISNQLTLGRTESNII
                     ENVSGLTKDAISKEIKAREILQKKLGIKLEDKIFRSIGTLENSRVMSSAEAMSHLSNI
                     KMGIEMNYIDKLDLRAIEQLMIGIQPAHQSIMYKSDDVENRDINRATYIRETLEKLRG
                     GMNCELQ"
     gene            10278..12725
                     /locus_tag="KR250_002944"
     CDS             10278..12725
                     /locus_tag="KR250_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HBH0068843.1"
                     /translation="MNFNRFTQRAKKAIDLAFESAKSLGHNIVGSEHILLGLLREEEG
                     IAAKVLSKVGFTEAYLEGKIVDMEGKGEEISEDIVLSPRSKQILELSGMFANKLKTNY
                     IGTEHILLAIIQEGEGIANKILNYAGVNDRTLAQLTIDMMGISDKNQYKAENSYTGNQ
                     NQAESKVLDKYGRNLTLYAKQNKIDPVIGREKEIQRVIQILSRRTKNNPVLIGDPGVG
                     KTAIAEGLATNIALGNVPETLKNKTLYSLEMGSLLAGAKYRGEFEERIKEVVDEVVKN
                     GNIILFIDEMHTIIGAGSTGEGSIDASNILKPALARGEIQVIGATTIDEYRKHVEKDS
                     ALERRFQPVMVDEPTKEDSIKILEGLRDKYEAHHKVKITDDAIKAAVELSTRYISDRY
                     LPDKAIDLIDEAASKVRLKENTPPSEIKKLELEIENIDKEKEEAVRCQDFEKAAKIRD
                     EQGLLKKQLEDVRERWNKSSKHSDLVDGEVIAEVVGLWTGIPVNKILEEEADRLLRLE
                     EILHNRVIGQEQAVKSISKAIRRSRAGLKDPNRPIGSFLFLGPTGVGKTELSKALAEV
                     QFGDENQIIRIDMSEYMEKHAVSRMIGSPPGYVGHDEGGQLTEKVRRNPYSVILFDEI
                     EKAHPDVFNILLQILDDGRLTDSKGRTVDFKNTIVIMTSNVGASTIGRQKTLGFSIAK
                     GDEEEKSQYEKMKENIMGELKQRFRPEFLNRIDDIIVFHSLNENHISKIVLLMAAKLQ
                     ERLKEMDIKLEMSDEAVKLISKSGFDLEYGARPLKRALQKELEDELSEAILKGNVKKG
                     SNVVAKVKDEKIVFETK"
     gene            12905..14278
                     /gene="radA"
                     /locus_tag="KR250_002945"
     CDS             12905..14278
                     /gene="radA"
                     /locus_tag="KR250_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HBH0068844.1"
                     /translation="MAKIKTKYVCQSCGYETAKWLGKCPECTKWNTFVEEIDQKSTKK
                     EVFIIDKSSSKPVSINSIESKEEERFTTDINELDRVLGGGIVKGSLVLVGGDPGIGKS
                     TLLIQVSSNVANLGKTVLYITGEESESQIKMRAKRLGINSENLYIFAENNLSIIESYL
                     ESVNPELIILDSIQTVFSPEISSAPGTVSQIKEGTSKFMKISKKMGISTFIVGHVTKE
                     GSLAGPKLLEHMVDTVLYFEGERYNTYRLVRAVKNRFGSTNELGVFEMRDLGLVELDN
                     PSKILISEKPKDVAGSVIISTVEGTRPMLLELQALVSPTSFGIPKRTSTGVDYNRVGM
                     LLAVLEKRVGLQIQNQDVYINIVGGIKINEPSIDLGIAISVASSFRNIPIDEDIAVTG
                     EVGLTGEVRAVSFIEKRIAECKKLGFKKIVVPRSNYDVVKETKGIEIWPVDNLRQAIN
                     IVLGRNQ"
     gene            14281..15351
                     /gene="disA"
                     /locus_tag="KR250_002946"
     CDS             14281..15351
                     /gene="disA"
                     /locus_tag="KR250_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA integrity scanning protein DisA"
                     /protein_id="HBH0068845.1"
                     /translation="MENFLDNKNMLYALKMISPGTPLRLGLNNVLRAKTGGLIVIATN
                     EDVMKIVDGGFAINAEYSPSYLYELAKMDGAIVLSGDVKKILFANAQLIPDYFIETSE
                     TGTRHRTAERVAKQTGAIVIGISQRRNVITVYRGNEKYVVEDISKIFTKANQAIQTLE
                     KYKTVLDQAVTNLNALEFNDLVTIYDVALVMQKMEMVMRVTSIIEKYVIELGDEGTLV
                     SMQLEELMGTTRIDQKLIFKDYNKENTEIKELMKKVKNLNSEELIELVNMAKLLGYSG
                     FSESMDMPIKTRGYRILSKIHRLPTAIIENLVNYFENFQQILDASIEELDEVEGIGEI
                     RATYIKNGLIKMKQLVLLDRHI"
     gene            15440..16534
                     /locus_tag="KR250_002947"
     CDS             15440..16534
                     /locus_tag="KR250_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HBH0068846.1"
                     /translation="MIRKVTRILFILLGFTIGITTYLTLMKDFEILTFGKETYGYIAA
                     VVAGIIIAILGYLIEPWVVNKVKEIAKIVDKELSKYPQTDILLGSMGLIVGFVIAYLL
                     SGLVNRIPIVGGILSLLLYLFLGYLGMKVALKSKNDLFNIGKLGRLANPIKDKDKDKE
                     NKKEVKAIPPKVLDTSVIIDGRIADICKTGFIEGKLIIPAFVLEELRHIADSSDDLKR
                     VRGRRGLDILNIIQKELNIEVEISERDFDDIAEVDSKLLKLAQVLNGKVVTNDYNLNK
                     VAQFQGVEVLNINELANAIKPVAIPGEDMVVQVVKEGKEAQQGVAYLDDGTMIVVDGG
                     RKYMNETIKVLVTSVLQTPAGRMIFAKPKN"
     gene            16663..18840
                     /locus_tag="KR250_002948"
     CDS             16663..18840
                     /locus_tag="KR250_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0068847.1"
                     /translation="MRKKIISFLATFIIILTSASQYSFADDISTRGKVIFIDMNRTSM
                     SNMLRIKSLREELDNRGYIGLMNIRGDKGSDDRRSYASMGAGGRANVANEEDINFESS
                     SKDRNIVFESATGKSAKGINNLTINKSINENLNFGEYGSVLGSLGQSLSDNGLKASVL
                     GNSDIIENGQLIKNRNLCLTAMDEYGRIPNGNVDTINKKDLSMPYGISTDYDKLIVET
                     KETYKNNDVIFVELGDTYRLDLYKPNLNEKTYESMKDNIEKNIDIYLKNIFSMVEEND
                     TIYIASAFPSDLDYKNKRRLSPIIKLNGEGKGILSSSTTRREGIVTNLDVGVEILDNF
                     NIKNQNMVGRKYELINRDDNKEFLMDEYQKIVSISSIRSTILNGFVSIVFLSWIVAMI
                     AILFRKHISKNYKETTFFILKELLKIGIVMPLSFMITPIMNFKTPLAISLGIIIITLT
                     IYLISKVLIKNDLKNMLFFTGLTIVIMVIDAGFGSYLMKSNVMSYDCIIGARYYGVGN
                     EYQGVAIGSAIFTFAILLTYKNIPKWYIIVFSLVILITSASPIMGANVGSAISECVAF
                     LLFILLIYNVKIDFKKIVLLGIAVVFVLGVFVAIDMILGSNSHLGMFVKEIYFNGPGE
                     IIQTFSRKIEMNLKLAQTSAWVNILLTGIGVILVLMINQIRYFKQLMDEYPIVFKGFI
                     ASIAGCLVTLLVNDSGIVSSATAFIYVIVPMITLSINLTALKE"
BASE COUNT         7801 a         2134 c         3633 g         5590 t
ORIGIN      
        1 ccccttccag gtcgccaaaa taaaatgctg gtgtggctca acggtagagc agctgacttg
       61 taatcagcag gttgtaggtt cgattcctat caccagctcc atataaaatt aatacggagg
      121 atttcccgag cggccaaagg gggcagactg taaatctgtt gtcatcgact tcggtggttc
      181 gaatccacca tcctccacca aaaaaatgcg cggatgtggc ggaattggca gacgcactag
      241 acttaggatc tagcgccatt ggcgtggggg ttcgactccc ttcatccgca ccatttttat
      301 ttaatttaat aaacgagcgg gaatagttca gtggtagagc gcaaccttgc caaggttgaa
      361 gtcgcgagtt cgaatctcgt ttcccgctcc aaattttatg tttatgttgt aatgtgggtg
      421 catagctcag ctggatagag caacgccctt ctaaggcgtg tgtccggggt tcgaatccct
      481 gtgcgctcac cacatatata ttggggatta gccaagtcgg taaggcacat gactttgact
      541 catgtatgcg taggttcgag tcctgcatcc ccagccaaac agtatggtct attagctcag
      601 tcggtagagc acctgacttt taatcagggt gtcccgcgtt cgagtcgcgg atagatcacc
      661 aatggagagg tgtccgagtg gtttaaggag ctggtcttga aaaccagtga cttcgaaagg
      721 ggccgtgggt tcgaatccca ccctctccgc caaatgccca gatagctcag tcggtagagc
      781 aggggactga aaatccccgt gtcggtggtt cgattccgcc tctgggcacc aaaatgtggc
      841 ggtatagctt agttggctag agcgttcggt tcatacccga aaggtcatag gttcgactcc
      901 tattaccgct actaaaattt aatgtgggcc attagctcag ttggttagag cgcccggctc
      961 ataaccggta ggtctggggt tcgagtccct gatggcccac cacattaaat taacttatgg
     1021 tgggtatagc tcagttggtt agagcgccag attgtggctc tggaggtcgt gagttcgact
     1081 ctcattatcc accccaactt atatggtcta ttagctcagt cggtagagca cctgactttt
     1141 aatcagggtg tcccgcgttc gagtcgcgga tagatcacca aaaaggcggc gacatagcca
     1201 agtggtaagg cagtggactg caactccttg atccccagtt cgaatctggg tgtcgcctcc
     1261 aataaaatgc gctcatagct caactggata gagtgtctga ctacgaatca gaaggttagg
     1321 ggttcgagtc cctttgggcg caccatatga atagcgaggg attatagctc agctgggaga
     1381 gcacctgcct tacaagcagg gggtcacagg ttcgagccct gttaatccca ccagtgcaaa
     1441 cctctagtaa aactactaga ggttttattt ttgattgaaa aagtttttat atttaggagg
     1501 taaaaaattt gtatatagta ataggaaatg aaatagttga tagtgaagaa cttaaaataa
     1561 ttatagataa aaatagtgaa tttaaagttg aaaaagattt aagtaaaagc acaaaaagag
     1621 aagatgtaat agcatatcag ttaagtatag atttaaatta cctagattct cttataaatg
     1681 agcaatgtaa tttagccagt ctatctgatg aagagaaatt tgatgaatat atgactttgt
     1741 ctgatgaact tgcactggat ttagaagagt taatgccaaa atatacaata ataaatgcta
     1801 gagcatataa attagacgag gtagatggaa ttgtaaaaat aatactagct gtagcgtatg
     1861 cagatttagg gcacttaaaa ctttctgatg tagtaaagag gctatctaga caagtagatt
     1921 aggatatgaa tatgaactat attaaaaaaa tgtataaaat cttacgtaaa gatgaaaata
     1981 attatgtaaa catagaaaaa aacagtataa aatcaagtca attgtagaaa ttttcacata
     2041 aattcatctg aaattctgta aaaaatgcct taattttaat gaggtaaatc gagtttacac
     2101 agtctaaaaa ttacttatat ttataaattt atcttattaa ttaaggaact ttcttaaaca
     2161 tcttcaaaaa taaagtattt tagtctataa tctttaatat tacacaaaca ataaaggaga
     2221 agaatagagt atgcttaaaa agtttaataa aatactagtg gctaatagag gcgaaatagc
     2281 tataagaata tttagagcct gttcagaatt ggggataaaa agtgttggta tatacagtaa
     2341 ggaagataag tatggattgt ttagaacaaa agcggatgaa tcatatttga taggagaagg
     2401 taaagggcca atagatgcat atttagatat ggatggaata atagacctag caaagagaaa
     2461 aaaagttgat gctatacatc caggatatgg ttttctagcg gagaatgcag aatttgctcg
     2521 taaatgtgaa gagaatggaa ttacttttat aggaccatca tctaaggtaa tgaatatgat
     2581 gggggataag attaactcta aaaaaatcgc aaaagaagtt aatgttcaaa ctataccagg
     2641 agtagaaaaa gcaataagaa gtactgaaga agcaaaggaa gttgcaaaca aaataggata
     2701 tcctgttatg ataaaagcat ctaatggcgg tggcggtaga ggtatgagaa ttgtacatcg
     2761 cgaagaagac ttagagcttg aatatgaaac tgcttgtagt gaatcaagaa aagcttttgg
     2821 agaagatatt atatttatag aaaaatatat agctgatcca aaacatatag aagttcaaat
     2881 attaggtgat aattatggaa atatagttca cttatatgaa agagattgtt cagttcaaag
     2941 aagacatcaa aaaataatag aatatgcacc agctttttcg ttagatgata aagtaagaaa
     3001 agagatatgt gaagatgcag ttaagttatc aaaacatgtg ggttattcta atgcgggaac
     3061 attagaattt ttagtagatg caaatggtgg gcattatttt atagagatga acacaagagt
     3121 acaggttgaa catactgtaa cagagatggt tacaggaata gatatagttc aaagtcaaat
     3181 actaatagcc caaggttaca gcttagatag tgaagaaatt aatataaaat cacaagatga
     3241 tgtggaaata agaggttact ctatccaatg tagaataact acagaagacc ctaagaataa
     3301 attcatgcct gatacaggta aaattcaagt ttatagaact ggttcaggat ttggtataag
     3361 actagatgga ggaaatggat ttacaggggc aaacataagt cctcattatg atagtttatt
     3421 ggttaagaca atatcttggg atagaacatt ccaaggagca ataaataaaa ctataagatc
     3481 tataaaagaa cttagagtta gaggtgttaa aactaatgta ggattcctag ttaatgtttt
     3541 aaataatcct atattctcaa atggtaaatg tagtactaag tttatagatg aaaatccaga
     3601 tttatttgaa ataacagaaa gtaaagacag aggaacaaaa ttacttcaat ttataggaga
     3661 tgtaattgta aatgataatg cttgtaaaga aaaaccatta tttgatgcat tacatgaccc
     3721 aagaatggat aaggatggtt caaaatcaga aggaagtaaa atacttttcg ataagttagg
     3781 taaatctgca tatatagaaa agataaaaaa tgataagaaa ttactattaa cagatacaac
     3841 aatgagagat gcacatcaat cattattagc aacaagaata agaacatatg acttattaaa
     3901 agctgctaag ccaacagaaa aatatcaaaa ggatttattc tcacttgaaa tgtggggagg
     3961 agctacttat gatgttgcat atagattttt aaaagaatct ccttggcgta gattacaaaa
     4021 gctaagagaa gaaataccaa gcataatgtt ccaaatgttg cttagagcat caaatggagt
     4081 tggatataaa aactatccag ataatgtaat agaagaattt acaaaagaat ctgctagaca
     4141 aggtatagat gtatttagaa tatttgattc attaaactgg gttgaaaata tgaaaccatc
     4201 tattaataca gctttagaaa ctggaaaaat tgttgaagca actatgtgtt atacaggtga
     4261 tatactagat aagactaaaa ctaaatacaa tctagaatat tatattaaaa tggcacaaga
     4321 attagaaagc ttgggagcag atataatagc aataaaagat atgtcaggtc ttcttaaacc
     4381 atattcagct tacacactag ttaaagagtt aaagaaaaat gtaaaagcac caatacattt
     4441 acacactcat gatacaagtg gaaatggtgt agcaacttgt ttaatggcgt cagaagctgg
     4501 agtagacata attgatgctg cattagaatc tatggcagga cttacaagtc agccatcact
     4561 taatgctata gtagaggcac ttaagaatac agagagagat acaggaatag atttatttgg
     4621 atatgatgag ttaggaaaat attataagga tttaagaaaa gtttataata aatttgaaag
     4681 tgatttaact aattcttgtg cagaaatata taattttgaa ataccaggag gccaatatac
     4741 aaacttaaaa cctcaagctg atagtttagg attagttaat agatttgatg aagttaaaga
     4801 aaaatataaa gaagctaatg aagttgtagg agatataata aaagtaactc catcttcaaa
     4861 agttgtagga gacttagcta tctttatgac taaaaataaa ttagataaag ataatataat
     4921 agaagaaggt aaaaatttat cattcccaga ttctgttgtg gattactgta aaggtatgat
     4981 tggtcaacca gaaggtggaa taccaaaaga tttacaagag gtagttctaa aaggtgaaga
     5041 agctataaca gtaagaccag gttcattatt accagcagaa gattttgatg aaatagctaa
     5101 atatctaaat gaaaagtatg atataaatgc taatataaga aatgttataa gttatgcttt
     5161 atatccaaaa gtatatgaag attatatcaa acatctacaa cattataatg atatatctaa
     5221 acttgaaagt gatgtattct tctatggatt aaataaaaat gaagaatgtg aagtagaaat
     5281 agaagaaggt aaggtattaa caataagatt agttgaaatt ggtgaagtta aggaaaatgg
     5341 atttagaact ataggatttg agttaaatgg aatggttaga gaagtagaaa taaaagataa
     5401 gaatttctct ggaaaaatta ataatgttga aaaagctgat atgaatgacc ctcttcaaat
     5461 tggagcaagc ataccaggaa aagttataaa aataatggtt aaagaagaag atgaagtaaa
     5521 agcaaatcaa ccattaatcg ttatagaggc tatgaagatg gaaactataa tagttgctaa
     5581 gactgatgga gtaattaagt caatcaaagt aaaagaagat gatatggttg aagataaaca
     5641 attattaatg ataatgaagt aatttaatta aaataaatta aaaagcactc tgaaatgttt
     5701 aataaaaaat gttttagagt gtttttattt attttaatta aatatttgtt aaaaaaaaga
     5761 ggacaattac ctccagatgt agaaataaag ctagtaagga aaatgttatc taaaaattct
     5821 aaatatagta aatattttcc ataatatttc ttaggaaata acagcaaaga taaaatacat
     5881 aaatttaaaa ggtgggtgct tatatgaagg tttatgatag taaaatgttg agaaatgtag
     5941 cggtattagg acatagcgga tgtggaaaaa caaatctaat cgaaactata gcttacacag
     6001 caaacactaa taagatacct aaattaacag acaaggtaaa tatgacttat agcatgggat
     6061 taatacctat agagtataat gattataagt ttaacttatt ggatacccca ggatattttg
     6121 actttagcgg agacgttgtg tcatctctta gagcaagtga tgcagcaata atagtaatag
     6181 atgcaacagc gcctattcaa gttggtacag agaaatcatt agaactaact gagagtattc
     6241 ctaagataat gtttataaat aaaatagata atgaaaaggc aagatacaaa gatgcaatag
     6301 caatgttaag agaaaaatac aataataaga tagttcctat gataagtcct atatacaaag
     6361 ataaaaactt tgtaaagtta cataatgttt ttgaaaacat agatgattta gaaggtgaat
     6421 ttaaggaaca agctatgagt gttaaagaag cattaatgga acttatagca gaaactgatg
     6481 accaaatatt agataaatat tttaatggag aagaactaac tacagaagag atacaaaaag
     6541 gtattattat aggtatacaa agaggtgata ttatacctgt aatatgtggt tcaactataa
     6601 ataatatagg cacaaaggaa atattagata caatatcaag ttatttagaa cctatcttta
     6661 cagaggaaag caaaccattt aggggattag tatttaaaac tatggttgac ccatttgttg
     6721 gaaagatgtc atatataaaa attactgagg gtgtactttc taaggataaa gatgttttta
     6781 atataaataa aaatgtaaaa gaaaaaatag ctaatatata tacattgaga aatagtgaat
     6841 tagtagaaat agaaaaagct aaagctggtg atatagtagt tataactaaa gttaattctc
     6901 taaaaacagg agacactata tctgcagata aagatgcaga agctttagaa aagatagatt
     6961 ttcctaaacc acaaatatat tatgcagtaa ctcctaagaa taaaggtgat gaagaaaaag
     7021 tagcatctgt attaaataaa ctagtagaag aggaccctac attacattgg tatagaaata
     7081 cagagacaaa acaagcactt ttaggaggtc aaggagaact tcatataaaa acaattaaaa
     7141 ataaaatgaa agataagttt ggtgttgatg ttgaattgaa tgaccttaaa gttccttata
     7201 gagaaactat aaaaggaaca gctgatgtac aaggaaaaca taaaaagcaa tcaggtggtc
     7261 atggtcaata tggagacgta aaaataagat ttgaaagatg tgaatctgat tttgaattta
     7321 cagaagaaat atttggtggt tctgtaccaa aacaatatat accagcagtt gaaaaaggtt
     7381 taaaagactc tatgcaaaaa ggtatattag caggatatcc tgttacaaac ataaaagcta
     7441 cgctttatga tggttcatac catgatgtag actcttctga aatggccttt aagatggcag
     7501 caagtgcagc ctttaaaaaa ggtatggaag aagcacaccc aatactacta gaacctataa
     7561 tgaaacttaa gataactgtt ccagaagaat atatgggaga tgtcatgggt gatataaata
     7621 agagaagagg aaaaatattt ggaatggaac cagatgacaa agggaaacaa ataatatttg
     7681 cagaagctcc acaagcagag acatttaaat atgcaataga tttaagagca atgacacaag
     7741 gtagaggata ctttgaaatg gaattagaga gatatggaga ggttccatca cagtttgcag
     7801 aaaaaataat tgggttagct actgcaaagt aatgaacaaa ataaatagaa aaataattaa
     7861 tatatattat taaaaatatt tttagggttt taactaggta taggtttatg ttaggtaaac
     7921 ttatacctag tttttataca tttttgatga ataaaaaaat atataatact ttcttaaata
     7981 ttttaataaa aagaaagtat ttttttatta ttatttttac aaatgtttag taataaagat
     8041 gagaaggtat atttaaaata taaagtattt aaagttctga aatctaatca tgaagcaaaa
     8101 aattagcatt tggttatcaa taaatataat aaataaaaac tcataattaa tgaaagaata
     8161 aattcatgtc taaatttcta taaaaaattt aaatttatac aatttagggc tttaaaaatt
     8221 taaataatag gtgtaaaata atagtcaaag aaagtcaaaa ataaaaaggt gtgtggtgat
     8281 attaatggca actatgacag atataattga aaaatttatt aaagatttga tggaagaaga
     8341 taatagcata caaattcaaa gaaatgaact agcaaacttg tttagttgtg caccatcaca
     8401 aataaattat gtcttaacaa caagatttac aatagataga ggatattaca ttgaaagtaa
     8461 aaaaggtggc ggaggatatg ttcaaataga aaaaatacga aaaagtaaag atgggcatat
     8521 tagagaacta ctaaatgaaa aaataggaag tcagatatcc tataaaaaag caaaagaatt
     8581 attagaaggt ttaaaagagt cggatttgat taacgaaaga gaattaaaat taatattata
     8641 tgcaatagat gataaatcat tgtgtatgcc aatatatgaa ttaaaagaga aggtaaggtc
     8701 taatatttta aagaatataa taataggatt atttagtata gaggagtgat aatttatgct
     8761 atgtcaaaaa tgcaataaga ataaagcatc tgtatattat aataagatag tcaatggaga
     8821 aaaaacagag atgtatttat gtagtgagtg tgcaaaagag aatacagaaa tgaattttaa
     8881 cttagatatg cctttttcaa tgatggatat attttctaat ttaggttttc aacctaaaaa
     8941 agaacttgag gaaaagctag tttgtcctaa atgcaataca acatatagtg agtttaagaa
     9001 taatgggaga tttggatgta gtgaatgtta taatgcattt agtagtcaag taaatccaat
     9061 gcttcaaaat atacatgggc atatagaaca tacaggaaaa gcacctaaaa aatcttttta
     9121 taagataagt gtagaaaatg agattaaaga actaaaagaa gatttagata gagctataaa
     9181 aaatgaagag tatgaattag cagctcaatt tagagataaa attaaatatt taaaaggttc
     9241 tattgactaa gattggagtg atattatgaa agagaacata gtcatgaaat caagagttag
     9301 gctagctaga aatttaaata actatccatt tccaaataaa ttagataaag aatgtgccat
     9361 ggaaataata gaaaaagtaa aaaatgcgtt tattaattct aacttagaac aaaaagaaga
     9421 atttgatttc tataaaattg aagatttaga ccaatctaaa aaaatgctta tggttgaaga
     9481 acatataata agcccagatt tagcagaaaa tgataaaagt gcagttatag taaaaaaaga
     9541 taaaactata agtataatga taaatgaaga agaccatata aggatacaaa ctatatgtga
     9601 tgatttaaac ttagaatatg catatagtgt agcaaatgaa atagatgatt tgttagaatc
     9661 aagtttagaa tatgccttta acacaaagct aggttatcta acatcttgtc caactaatac
     9721 aggaacagga atgagagctt ctgtaatgat gcatttacca gcattaagtc aactaggcta
     9781 tatggatgaa ctttataaaa tttcatcaca aataggtata gcaattagag ggatatatgg
     9841 agaacgaaca gaagcattgg gaaatatata tcaaatatct aatcaactta cacttggaag
     9901 aacagagtct aatattatag aaaatgtaag tggacttaca aaagatgcaa tttcaaaaga
     9961 aataaaagct agagaaatat tgcaaaaaaa attaggtata aaactagaag ataaaatatt
    10021 tagatcgata gggacacttg aaaattcaag agtaatgagt agcgcagagg caatgagcca
    10081 tctatcaaat ataaaaatgg gaatagaaat gaactatata gataaactag atttaagggc
    10141 tatagaacaa cttatgatag gtatacaacc agcgcatcag tcaataatgt ataagagtga
    10201 tgatgttgag aatagagata taaatagagc aacatatatt agagaaactt tggaaaaatt
    10261 aagaggaggg atgaattgtg aacttcaata gatttacaca aagagctaaa aaggcaattg
    10321 atttagcgtt tgaatctgct aaaagtctag gacacaatat tgtcggaagt gaacatatac
    10381 ttttaggtct tttaagagaa gaagaaggta tagctgctaa agttctaagt aaagttggat
    10441 ttacagaagc ttatttagaa ggcaaaatag ttgatatgga aggtaaggga gaagaaatct
    10501 cagaagatat agtattaagt ccaagaagta aacaaatact tgaattatca ggaatgtttg
    10561 caaataaatt aaaaacaaat tatattggga ctgagcatat attattagca attattcaag
    10621 aaggtgaagg aatagctaat aagattttaa attatgctgg agtaaatgat aggactttag
    10681 cccaattaac aattgatatg atgggtatta gtgataaaaa tcaatacaaa gcagaaaata
    10741 gttacacggg caaccagaat caagcagaat ctaaagtact tgataagtat ggaagaaatc
    10801 ttacactata tgcaaaacag aataaaatag accctgtaat aggtagagaa aaagaaatac
    10861 aaagagtaat acaaatatta agtagaagaa ctaaaaacaa tccagtgttg ataggagacc
    10921 caggtgtagg gaaaacagcc atagctgaag gtctggctac aaatatagcc ttaggaaatg
    10981 ttccagaaac actaaaaaac aaaactttat actctttaga gatgggttca ttattggctg
    11041 gggctaagta tagaggagaa tttgaagaaa gaattaaaga agttgtagat gaagttgtta
    11101 aaaatggaaa cataatctta tttatagatg agatgcacac aataataggg gctgggtcta
    11161 ctggagaagg ctctatagat gcttcaaata tattaaagcc agccttagca agaggagaaa
    11221 tacaagttat aggtgcaact acaattgatg aatatagaaa gcatgttgaa aaagattctg
    11281 ctctagaaag aagattccaa ccagttatgg tagatgaacc aactaaagag gactcaataa
    11341 aaatattaga aggattaaga gataaatatg aagctcatca taaagttaaa ataactgatg
    11401 atgctataaa ggcagctgta gaattatcaa ctagatatat atcagacaga tatctgccag
    11461 ataaagctat agacttgatt gatgaagctg catcaaaagt aagattaaaa gaaaatacac
    11521 ctccttcaga aataaaaaaa ttagaactag aaatagaaaa tatagataaa gaaaaagaag
    11581 aagcagtaag atgtcaagac tttgagaaag cagccaaaat aagagatgaa caaggtctac
    11641 ttaaaaaaca attagaagat gttagagaaa gatggaacaa gtcatctaaa cattcagatt
    11701 tagttgatgg agaagttatt gctgaagtag tgggcttatg gacaggaata ccagttaata
    11761 aaattcttga agaagaggct gatagacttt tgagacttga ggaaatactg cacaatagag
    11821 ttataggtca agaacaagca gtaaaatcta tttcaaaggc aatcagaagg tcaagagcag
    11881 gtcttaaaga ccctaataga ccaattggtt catttttatt tttaggtcca acaggagtag
    11941 gtaaaacaga attatctaag gcattagcag aagtgcaatt tggagatgaa aatcaaataa
    12001 ttagaataga tatgtctgaa tatatggaaa aacatgctgt gtcaagaatg ataggttcac
    12061 ctccaggtta tgtaggtcat gatgaaggtg gtcaattaac tgaaaaagta agaagaaatc
    12121 catattcagt tatattattt gatgagatag aaaaagctca tccagatgta tttaatattc
    12181 tgttacaaat tctagatgat ggtagactaa ctgattcaaa aggaagaact gtagatttta
    12241 agaatactat tgtaataatg acatcaaatg ttggagcatc tacaattggt agacaaaaaa
    12301 ctttaggatt tagtatagct aaaggagacg aagaagaaaa atctcaatat gaaaaaatga
    12361 aagaaaatat aatgggtgaa ttaaaacaaa gattcagacc agagttttta aacagaatag
    12421 atgatataat tgttttccac tcactaaatg aaaatcatat atcaaaaata gtacttttaa
    12481 tggcagcgaa actacaagag agattaaaag aaatggatat aaaattagaa atgagtgatg
    12541 aggctgttaa attaatttct aaatctggat ttgacttaga atatggagca agacctctta
    12601 aaagagcttt acaaaaagag ttggaagatg agctatctga agcaatctta aaaggtaatg
    12661 tgaaaaaggg aagtaatgta gtagcaaaag tcaaagatga aaaaatagtt tttgaaacta
    12721 aataatttaa tagagttgca ttttaaaata ttgcaaatat aagttttata atctaaacaa
    12781 aattttaagg gcaatgaatc tataataatt cattgtcctt aattatttta tttaatcttt
    12841 acatattgtc tttcagatat gctaaaatta tagtatagaa taaaaaatta aggatgataa
    12901 aagtatggca aaaataaaaa caaaatatgt atgtcaatct tgcggatatg aaacagctaa
    12961 atggcttggt aaatgtccag aatgtacaaa atggaataca tttgtagaag aaatagacca
    13021 aaaaagcacc aaaaaagagg tctttataat agataaatct tcatctaaac cagtcagtat
    13081 aaactcaata gaaagtaaag aagaagagcg gtttacaaca gatataaatg agttagatag
    13141 agtgcttgga ggaggtatag ttaaaggctc tttagtgctt gttggtggtg accctggtat
    13201 aggtaaatca acacttttaa tccaagtatc tagtaatgtt gccaatttag gaaaaactgt
    13261 tttatatata actggagaag aatcagaatc tcaaataaaa atgagagcta aaagattagg
    13321 tattaactca gaaaatttat atatatttgc tgaaaataat ttaagtataa ttgaatctta
    13381 tttagaaagt gtaaatccag aattaataat tctagactca attcaaactg tcttcagtcc
    13441 agaaatatcc tcggcaccag gaactgtcag ccaaattaaa gaaggtactt caaaattcat
    13501 gaaaatttct aaaaaaatgg gaatttcaac gttcatagtt gggcatgtta caaaagaagg
    13561 ttcactagca ggaccaaaat tattagagca tatggttgat actgttctat attttgaagg
    13621 agaaagatat aatacttata gattagtaag agctgttaaa aatcgatttg gttcaactaa
    13681 tgaacttgga gtatttgaaa tgagggattt aggattagta gaacttgata atccatctaa
    13741 aattttaatc tctgaaaaac caaaagatgt agcaggttca gtaataatat ctactgtaga
    13801 aggtacgaga ccaatgttac ttgaattgca agcattagtt tctcctacaa gttttggtat
    13861 accaaaaaga acatctacag gagtcgatta caatagagta ggtatgttat tagcagtatt
    13921 agaaaaacgt gttggtttgc aaatacaaaa tcaagatgtc tatataaaca tagttggagg
    13981 aataaagata aatgaaccct caattgattt agggatagcc atttctgtag catctagttt
    14041 tagaaatata ccgatagatg aagatatagc tgttacagga gaagtgggac ttacaggaga
    14101 agttagagca gtaagcttta tagaaaagag aatagctgag tgtaaaaaac ttggatttaa
    14161 aaagatagta gttcctagaa gtaattatga tgttgtaaaa gagaccaaag gcatagaaat
    14221 atggccagta gataacttaa gacaagctat aaatatagta cttgggagga atcaataaat
    14281 atggagaatt ttctagataa taaaaatatg ctatatgcat taaaaatgat atctcctgga
    14341 actccactta gattaggtct aaacaatgta ctaagagcta agactggtgg attaattgta
    14401 attgcaacaa acgaagatgt aatgaaaata gtagatggag gatttgctat aaatgcagaa
    14461 tattcaccat catatctata tgaattagct aaaatggatg gagctatagt tttaagtggt
    14521 gatgtaaaga aaatattatt tgctaatgca caacttatac ctgactattt tatagaaaca
    14581 tcagagacag gaacaagaca tagaacagca gaaagagtag caaaacaaac tggtgctata
    14641 gtcataggaa tttcacaaag aagaaatgtt ataacagttt atagaggaaa tgagaagtat
    14701 gtagtcgaag atatatctaa gatatttact aaggcaaatc aggctataca aactctggaa
    14761 aaatataaga cagtattgga ccaagctgta acaaatttaa atgccttaga gtttaatgat
    14821 ttggtaacta tttatgatgt tgcattagtc atgcaaaaga tggaaatggt aatgagagtt
    14881 acaagtataa ttgaaaaata tgtgatagaa ttgggtgatg aaggaacttt agtaagtatg
    14941 caattagaag aattaatggg tacaaccaga atagaccaga aattaatatt caaagattat
    15001 aataaagaaa acacagaaat aaaagaactt atgaaaaagg tcaaaaattt aaattcagaa
    15061 gaactaatag aattggtcaa tatggcaaaa ctattagggt atagtggttt ttcagaaagt
    15121 atggatatgc ctataaaaac aagaggttat aggattctta gcaaaataca tagactacca
    15181 acagcaataa tagaaaactt agtaaattat tttgaaaact ttcaacaaat tttagatgca
    15241 tctattgaag aattagatga ggttgaagga ataggtgaaa taagagcaac atatataaaa
    15301 aatggactca taaaaatgaa acaattagtc ttattagata gacacatatg atgaatttta
    15361 aatgaatttt actacttttt tcttgaatat aatatacact ttggtctata atagataagt
    15421 ataactaggg ggtgaagcat tgataaggaa agtaacaaga atattgttta ttttacttgg
    15481 atttacaata ggtataacaa cctatttaac attaatgaaa gattttgaaa tattaacatt
    15541 tggaaaagaa acatatggtt atatcgcagc tgtagtagca ggtataataa tagccatttt
    15601 aggatatctt atagaaccat gggttgtaaa taaagtaaaa gaaattgcta aaatagtgga
    15661 taaagaattg tcaaaatatc ctcagactga tatattgtta gggtctatgg gtcttatagt
    15721 aggatttgtt attgcgtatt tattaagtgg attagtaaat agaataccaa tagtaggtgg
    15781 aatactatcc ttactattat atttattcct aggatatctt ggaatgaaag ttgcgcttaa
    15841 aagtaaaaat gatttattta atattggaaa actaggaaga ttagcaaatc ctattaagga
    15901 taaagataag gataaagaaa ataaaaaaga agtaaaagcc attcctccaa aagtattaga
    15961 tacaagtgta ataattgatg gaagaatagc tgatatttgt aagactgggt ttatagaagg
    16021 aaaacttatt attcctgctt ttgttttgga ggaactaaga catattgcag attcttcaga
    16081 tgatttaaaa agagtaagag gtagaagagg tctggatata ttaaatataa ttcagaaaga
    16141 attaaatata gaagttgaaa taagtgagag agactttgat gatatagcag aagttgatag
    16201 taaattatta aagcttgctc aggttttaaa tggaaaagtt gttacaaatg attataactt
    16261 aaataaagtt gctcaattcc aaggtgttga agtattaaat ataaatgagt tagctaatgc
    16321 tataaaacca gttgctatac caggtgaaga tatggttgtt caagtggtta aagaaggaaa
    16381 agaggctcaa caaggtgttg catatctaga tgatggaact atgatagttg ttgatggagg
    16441 aagaaaatat atgaatgaga ctataaaagt actcgttact tctgttcttc aaactcctgc
    16501 aggtagaatg atatttgcaa aacctaaaaa ttaatatgtt caaagaaacc tataatttgg
    16561 gctgtttatt ttaaatagat ggtccaaatt tatttattta taacgataat gtttaatgta
    16621 ttcttaaaga tggggattta aatcagaaag cagggagaat ttatgagaaa aaagattata
    16681 tctttcttag caacatttat cataatatta acatcagcaa gtcagtatag ttttgcagat
    16741 gatatatcaa cacgtggaaa agtaatattt atagatatga atagaactag tatgagtaat
    16801 atgttaagaa taaaatcatt aagagaagaa ctagacaata gagggtatat tggcttgatg
    16861 aatataagag gcgataaagg aagtgatgat agaaggtcat atgctagtat gggtgctggt
    16921 ggtagagcta atgtggctaa tgaagaagat attaattttg aaagctcgag taaggataga
    16981 aatatagttt ttgaatcagc tacaggaaaa agtgcaaaag gtataaataa tttaactata
    17041 aataagtcta taaatgaaaa tttaaacttt ggagagtatg gttctgtatt agggtcatta
    17101 ggacaaagtt tatctgataa tggattaaaa gcatctgtat tgggtaattc agatataatt
    17161 gaaaatggtc aacttataaa aaatagaaac ttatgtctaa ctgcaatgga tgaatatgga
    17221 agaataccta atggaaatgt agatactata aataaaaaag acttatctat gccttatgga
    17281 atatcaactg attatgataa attgattgta gagactaaag aaacttacaa aaataatgat
    17341 gtgatttttg tggagcttgg agacacatat agattagatt tatataaacc aaatttaaat
    17401 gaaaaaacat atgagagtat gaaagataac atagaaaaaa atatagatat atatttaaaa
    17461 aatatatttt ctatggttga agaaaatgac actatctaca tagcaagtgc atttccaagt
    17521 gatttagatt ataaaaataa aagaagactt tctccaataa taaaattaaa tggagaaggg
    17581 aaaggtatat tatcatcttc aactactaga agagaaggta ttgtaacaaa tttagatgtt
    17641 ggggttgaaa tattagacaa ctttaatata aaaaatcaaa atatggttgg aagaaaatat
    17701 gaacttatta acagagatga taataaagaa tttttgatgg atgaatatca aaagatagta
    17761 tctatttcaa gcataaggtc tacaatactg aatggttttg taagtatagt tttcttgtca
    17821 tggatagttg ctatgattgc aattttattt agaaagcata tatcaaaaaa ttataaagaa
    17881 acaacatttt ttatattaaa ggaactttta aaaataggaa tagtaatgcc actgtcattt
    17941 atgataacac caataatgaa tttcaaaaca ccattagcaa tatctttagg aataattata
    18001 ataacactta ctatatatct tataagtaaa gttttaataa agaacgattt aaaaaacatg
    18061 ctgtttttta ctggattaac catagtaatt atggttattg atgctgggtt tggctcatat
    18121 ttaatgaaaa gcaatgttat gagttatgat tgtataattg gagctagata ttatggagta
    18181 ggaaatgagt atcaaggtgt ggcaatcggt agtgcaatat ttacatttgc aattttactt
    18241 acatataaaa atataccaaa gtggtatata atagtttttt ctttggttat cttaattaca
    18301 agtgcatctc caattatggg agcaaatgtt ggaagtgcta tttctgaatg tgttgcattt
    18361 ttactattta tactattgat atataatgta aagatagatt tcaaaaaaat cgtattatta
    18421 ggtatagcag tagtatttgt attaggagtt tttgtagcaa tagatatgat tttgggaagt
    18481 aattcacatt taggtatgtt tgtaaaagaa atttacttta atggaccagg agaaataata
    18541 caaacattta gtagaaagat agagatgaat ttaaagctag ctcaaaccag tgcatgggta
    18601 aatatactgt taactggaat aggtgtaatt ttagttctta tgattaatca gattagatac
    18661 tttaagcaac tgatggatga gtatccaatt gtatttaaag gatttatagc atcaatagca
    18721 ggatgtttag taactctttt agtgaatgac tctgggattg tgtcatcagc tacagccttt
    18781 atttatgtaa ttgtacctat gataacatta agtataaatc ttacagcatt aaaagaataa
    18841 gtactgtatg aataatatgt aaatattaat acattaattt aacttcttta tttacaaaat
    18901 aaagaagaaa gtttataaaa aataaattat aataaaaaag agtttatctc aaaattgatt
    18961 attctaatca aattttaaga taaacttttt ttattataaa gataaataac atataacctc
    19021 atattttagg tagatttttt tcttatatgt ttaggagtgc atttaaattt ttccttaaaa
    19081 attctgtaaa ataattttgt attagtaaat ccatgagctt ctaatatact gcataaggta
    19141 tcatttgtgt taattaaa
//
LOCUS       DAEPIN010000071        18766 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.071, whole genome shotgun sequence.
ACCESSION   DAEPIN010000071 DAEPIN010000000
VERSION     DAEPIN010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18766
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.071"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            543..4089
                     /locus_tag="KR250_002949"
                     /pseudo
     CDS             543..4089
                     /locus_tag="KR250_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453516.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            4274..6208
                     /locus_tag="KR250_002950"
     CDS             4274..6208
                     /locus_tag="KR250_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HBH0068848.1"
                     /translation="MENKFLPISKQDMIDRGWEELDFVLVTGDAYVDHHSFGTAIISR
                     VLESAGYKVGIIAQPDWRTTDDFMKLGKPRLAFLVNAGNMDSMVNHYSVSKKHREKDM
                     YSPGGKMGYRPDRATIVYCNKIREAYSDVAIVIGGIEASLRRFAHYDYWSDKVRKSML
                     IDSGADLLVYGMSEKQIVEVANALNDGYDPKYIRHIDGTCYISDTLEEIYDKYILIPS
                     YKEICEDKMKYVEAFKIQYDEQDPFRGNIIVQPHGSKYLVQNKPEKPLSREELDEVYG
                     LPYQKTYHPVYEKFGGIPAIEEVKFSIVSSRGCFGSCSFCAITFHQGRAVQSRSEKSI
                     IDEAIGITNLDDFKGYIHDVGGPTANFRRPACKKQITKGACKNRQCLSPSPCKNLDAD
                     HSEYLHLLRAVRKLPKIKKVFVRSGIRYDYVMADKNNKFLRELIEHHVSGQLKVAPEH
                     ISEEVLKYMQKPAGKTYDKFRQKFFAINEELGKKQYLIPYLMSSHPGSTLNSAIELAE
                     YLRDTHYQPEQVQDFYPTPGTLSTTMFYTGIDPLTMKPVYVPKSKRDKAMQRALLQYR
                     APRNYDLVYSALVEAGREDLIGFGHRCLIKPKNEKPYFNRNNSKKNVSKGTNKNKKTN
                     TNNRSNKNQQKSSTKKKKKH"
     gene            6314..7309
                     /locus_tag="KR250_002951"
     CDS             6314..7309
                     /locus_tag="KR250_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBH0068849.1"
                     /translation="MKKYISIILLVVLTMVVLVGCSPGKDNPKNKELSVVKQVKVAVP
                     DGLPAVAIAKLANENPEIKEGYETVYSIEKTPEAISTRVMKKDADIAIVPSNMAAIAY
                     NKTSSYNIVGTVGMGSLYLVSTENIKDYSDLVGKEVGCTGKGLTPDITIKSLLLQKDI
                     NYSDIKFNYVNSASELVPLLATGKVKTGIVPEPALSALMSKNPDIKIIKSLNDSWKEV
                     SGSKDGYPQSTLIVKSEFLRDNKDFVDSFVGQLSNSIDWANKNPEELGAYSEKIKIST
                     ESKIIGKSLERANLKYIPVKDMIKDYKNYYEKLANFDDKTLGGKVPDEAIYFVEK"
     gene            7281..8066
                     /locus_tag="KR250_002952"
     CDS             7281..8066
                     /locus_tag="KR250_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBH0068850.1"
                     /translation="MKQYTLLRNKKNKEQFIDKMEVTLSCVILLFLWQIIALKIDNDI
                     FLPTVGQVFNSIKEIMLNSSFYIDILYSMGRCFFSFLLAMIFAIILSIVSYLNRFFRN
                     LLKPINALTRSIPTMILVVLALIWFEKDSTPFIVGFTIVFPILYDNVLGAILNIDKNL
                     LEMANVYKIRFIDKVLKIYLPAIKFQIISILVSTFSLSLKVAIAGEVYSQPTYGIGTM
                     IQTEKINFNTSGIFAWIIIVLLISAILQIAQKFLARRAFLWKR"
     gene            8054..8752
                     /locus_tag="KR250_002953"
     CDS             8054..8752
                     /locus_tag="KR250_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0068851.1"
                     /translation="MEKIKLSIENINKRYDSRIIFRDFNIDFYVNEVNCILGKSGCGK
                     TTLLNIISGIIKNDTNNLNIKENLNRVGNKLEASYIFQDDRLIDWLTVEENIKIVVNK
                     YYNKTQLNKICDEYLELVGISDYKKFYPQMLSGGIRQRVNIARAFIYPSKNIIMDEPF
                     KSIDAKNTQLIMDNFRNILRKEKRTVLFVTHNIEEALFLADRIFILGDSPIRIKKILK
                     NSKELEKNEVLKLI"
     gene            8875..9348
                     /gene="ybaK"
                     /locus_tag="KR250_002954"
     CDS             8875..9348
                     /gene="ybaK"
                     /locus_tag="KR250_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HBH0068852.1"
                     /translation="MVKTNAMRILDSNKIDYKVMSYEVKSEHVDGVEVAHDIGRDVNE
                     VYKTLVTQGVSKNIYVYVIPVHENLDLKKAAKVAKEKSVEMIHVKDINKLTGYIRGGC
                     SPIGMKKLYKTFVNESAKNLDTIIVSAGKIGYQIELSPFDLQRLIKVEFVDVIKK"
     gene            9545..10384
                     /locus_tag="KR250_002955"
     CDS             9545..10384
                     /locus_tag="KR250_002955"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HBH0068853.1"
                     /translation="MRKIKSEQIVEQVKKLCIEASLYLGEDVLSCIKEKAKSEKSEVG
                     KNILNILVENAEIAKEKNIPICQDTGMAVFFVEIGQEVLIEGDTLTDAINEGVRQGYE
                     EGYLRKSVVSPINRVNTKDNTPAVIHYDMVKGDKIKIEFAAKGFGSENMSKMKMLKPS
                     DGLEGIKKFIIDTVSEAGPNPCPPMVIGVGIGGTVDKCAQIAKKALFRELGEFNKDEN
                     IAKLESELLTAINKLGIGPQGLGGTTTALGLNIETFPTHIAGLPVVVNINCHASRHKK
                     VVI"
     gene            10397..10945
                     /locus_tag="KR250_002956"
     CDS             10397..10945
                     /locus_tag="KR250_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S-containing hydro-lyase"
                     /protein_id="HBH0068854.1"
                     /translation="MIKITTPVNEIDIAKLNCGDTISLSGILYTARDAAHKRLIDCIN
                     KGEELPFDVYGQGIYYVGPTPTKPGEVIGAAGPTTSYRMDDLTIPLLERGLRLMIGKG
                     KRSDEVIEGMQKYGAVYLAAIGGAGAYISNSIKSCEIIAYEDLGAEAIRKIVVEDLKL
                     TVAIDSYGNNIYEQGRAIYECK"
     gene            11011..12192
                     /locus_tag="KR250_002957"
     CDS             11011..12192
                     /locus_tag="KR250_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HBH0068855.1"
                     /translation="MSKDYAKLALEMHEINKGKVSVESKVEVKTKDDLSTAYTPGVAE
                     PCLKIHENQDDVYRYTSKGNLVAVVSDGSAVLGLGNIGAEASIPVMEGKAILFKQFAD
                     VDAFPICLKTNDVDEIVKSVELMEPVFGGINLEDISSPRCFEIEERLKKSLSIPVFHD
                     DQHGTAIIVAAAIINSIKLIENKKIEDLEIVINGAGAAGIAIAKILLNMNVKNIILCD
                     RSGALEASIENLNYVQKEMLKVTNIRNEKGPLKDIIKGKDVFIGVSGPGAVTKEMVES
                     MSEKPIILAMANPAPEIMPEEAKLGGAFIMGTGRSDFPNQVNNVLAFPGIFRGALDVK
                     AKEINEEMKIAAAYAIANTISDEEICPEYILPDVFNKNVVKNVAKAVKEAAIKTKVNR
                     I"
     gene            12360..12746
                     /locus_tag="KR250_002958"
     CDS             12360..12746
                     /locus_tag="KR250_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009248914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBH0068856.1"
                     /translation="MEYKVNDQELNASMFIPFVNRIWPGDYDTDKTQTALSKTLNITA
                     YDDKVLVGCLRVLSDGYYFGTITELLVLPEYQKQGIGSKLFQLAKENTPTMLYFGAQP
                     GVEEFYEKNGCQRSLQSYIIKKKDSY"
     gene            12896..13159
                     /locus_tag="KR250_002959"
     CDS             12896..13159
                     /locus_tag="KR250_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBH0068857.1"
                     /translation="MEETKFCQCCAMPMGQTDELYGTNKDGSKSTDYCSYCFKDGEFT
                     ADISMEEMIEVCIPHMLQSNKDMTEDEARTMMNNFFPTLKRWK"
     gene            13685..14368
                     /locus_tag="KR250_002960"
     CDS             13685..14368
                     /locus_tag="KR250_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0068858.1"
                     /translation="MSSILLVEDDLSLIYGLEFSIQKSGFSIDIARTVKEAMQMYEEK
                     NYDLLLLDVSLPDGNGFQICEKVREVSNIPIIFLTASDEEVNVVMGLDMGGDDYITKP
                     FKLNELISRIKALLRRYNVASNVNELSSNNITIKLLENRVFKGKFEVELTTAEYKLLC
                     LFMKNKNIVLTRKNILDKLWDGNGSFIDDNTLSVYVRRLRNKIEDNPENPKFLLTVRR
                     MGYKWNVIK"
     gene            14379..15620
                     /locus_tag="KR250_002961"
     CDS             14379..15620
                     /locus_tag="KR250_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBH0068859.1"
                     /translation="MKIFSNKDIKIFFLMLISILVMFIIVGQIVVISITNNYKKTLIE
                     HDYNIAGYLYSIGVDKSKIPSAFVAHEDKYILKGKEILDTAGYGFDIDVSFTPSVKLF
                     QSKYIKTMSIYLLVFFTMIIFILYMYFFRQQSKIEEASYKIDLFMNGNINERLNTYEE
                     GSLSKLFSSIDVMSTSLNTHILKEKQSREFLKNTISDISHQLKTPLTALIMYNQILQE
                     ESHNSDVVEAFVQKSQSELERIETLIKNLLKITKIDSKTIILNKNNVNLKNLIVKILY
                     SFETRAEKEGKVISVNGSENTLLFCDYEWMSEALSNLIKNALDNTKENDKITIEWFET
                     TITTIVSVKDTGNGIHVEDIHHIFKRFYRSKFSKDNQGLGLGLPLVKSIVEQHNGTIS
                     VESKLSQGSIFTISFLKLTNM"
     gene            15692..16375
                     /locus_tag="KR250_002962"
     CDS             15692..16375
                     /locus_tag="KR250_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0068860.1"
                     /translation="MDILVVNNLCKTYGNGYTKVEALKDVSFTVSKGEFVTVVGESGS
                     GKSTLLNLLGGLDTPTSGRIFIDGKEICSMKEEKQTIFRRRNIGFIFQAYNLIPELTV
                     EQNIIFPLLLDYKKPDTIYLDEVLTILGLKDRKNHLPNQLSGGQQQRVAIGRALITKP
                     SIILADEPTGNLDSKNSNDVISLLKVTAEKYNQTIIMITHNTNIASTADRILQVSDGV
                     LTDLGVNRE"
     gene            16372..18702
                     /locus_tag="KR250_002963"
     CDS             16372..18702
                     /locus_tag="KR250_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0068861.1"
                     /translation="MRSYLELVPQYAKVHKKKNRTTILCIIIAVCLVTAIFGLADMAI
                     QCQKIQFIKDDGNWHIVIRNIDEKFISEINSRVDIAHHGRGQVVDNGEFKSKEILIQG
                     LDEEVAKQFNLEIIRGRYPIGENETLIDEDAVKSFNLKIGDFIKIKLADGRNKNYKIV
                     GEYSSISSLRATDKHGLILSILGTQAIKKSETYDYYYITFKEKVNIRKATSDIKNTFN
                     LSDKQIGKNERLLSIIGQGDSDFSKPLYLTAGLLFVLVLVASIIMIYNSFNMSVIERV
                     KFFGLLRCLGSSKHQVKKFVVLESLILSIKAIPIGLVLGSLVTIISSIFLKYVNELFS
                     SMPILKVSFIGIAFGIIVGFLTVILSAIIPAKKASRVSPLSAVRGNLMSKEILNNGKT
                     SKVANIGSFKIETSMGLNHAFSRKKSFVLMTSSFAISIILFLGFSVLIDLMYQGLKPM
                     KPEAPHISIVDPNFKSVLTKEDINEISNIKGVKKVYSRMMTDIEATYNGKKTKSQLIS
                     YEKYQFNWAKKYLLKGEVNENKLQSENLALVEEGHGFDIGDKIYIKNGNAEKELEVGG
                     ILSSIPFEAKDKYIGNIITSENTFTNITGIKGYIIMDIQVDKDTPGDLIENIRRVVDD
                     NLEVRDKRQSNLEVKNSFYTMATFVYGFVFIIAIISMFNIINSMNISVTSRKNYYGVM
                     RAVGISNRQLRRMVIVEAGSYAVSGCLLGSILGLILHRYMFISLVSSMFHLEWQVPFN
                     LLLITVLTMIIITLLSVRKPIKKICELDIIDSINAQ"
BASE COUNT         7508 a         1895 c         3354 g         6009 t
ORIGIN      
        1 aaagtgattt tgaaatttat attttatttt tataaatata attattatat ttataaaaat
       61 aaaatagtaa aaggatatta taatatttta ctagaattat tatctggtaa cttaagtttt
      121 gagtattaat ctaaagtata tgtttttata tggtttaaat tttatgtata caaaattaat
      181 ttgctaactg aatcatataa aaaaaaacaa aatatttttt agagggaatg attagataga
      241 atttttttgt tatgaaatta attgaagagt aattaattag aaaaggaatg aataataatg
      301 aatatattac attattactt tttgataaat caaaaataaa gttataattt ttaatattaa
      361 ttaagaatga tgaaatatga ataaagattt tacaaataaa tcttaagcta tcttatttaa
      421 ttctaataca ggaagaaggt aaatgtatat tgaaaaaatc atagaaatag aagtaaacaa
      481 tttaaataaa tacaaaggtt tgcaaaagaa gtaatataat tatatacaat gaggatgtga
      541 gtatgatgaa cagactaaag ccaagaattt taggtattag tatcttgttt attattataa
      601 ttattttaac atgtggagct gttttcaagt ttaaatttaa attagataat aaattaaaag
      661 aaaccacaca tacatatttg tctgagttta cagaacacaa tataattaat ctcaagacaa
      721 gacttagtgg tcaatttgac atgcttgaat ctattgctaa cttcatagga aacttgaata
      781 atataaatga tgaaatggtg attgatttaa tggattctga tgtaaagcga agttctttaa
      841 ttagaatgac tatctctaca ttagatgggg aatgttattc taatgatgga attacccata
      901 atgttcaaga tagagattat tttaaaaaag ctaaaaatgg agaaaagaat atatcagagc
      961 ctttaaaatc tattgttaat aataaagaaa taatagtttt atctacccca gtttataaaa
     1021 gtgggaaaat agttggtgtt ttaagtggaa tttatgattc aaaaaagtta aatgaattat
     1081 taggattatc tgcatttgat gaaaaagcag atgtttttgt tgcaaagaaa aatggagata
     1141 taattgctca aaaaacaaat aaaaatagta agacgatatc atgtatattt cagctttttg
     1201 acaaagataa ttgtgatgaa gttgattgtc taaaaactat aaaagaaaat atgaaaaatg
     1261 ataagtctga gtattttaat tataacttag ataatgaaaa atttatgtta aactatcagc
     1321 cacttggagt taatgattgg tatgtttttt ctgttgtgcc aaataaaata gtgtctattc
     1381 agtcagagaa tatatcttat gatgtatata tttttgcatg aaatactact agttatatta
     1441 atttttataa tatatatagc atgtattata aataataaca ccaaaattat tataaatgaa
     1501 aaagaaaaat ttaaagtact cactgataat atactaggag gggttaaaac ctgtttaaaa
     1561 gatgaatata tgactatgac atatgtaagt caggggtttt tagagcttac aggttactct
     1621 tgtgaagatt taaataaaat atttaaaaat agatttaaag acatgattta taaagatgat
     1681 ttagacaagt ttaattgtat tattctaaac caattagaaa aacaagataa aattgaagtt
     1741 gaatatagaa ttgtaagaaa agatggaagt ataatttgga ttttggagag aagtaagtct
     1801 ataaatgata ataaaggaag tgagtgtatt cagggagttt taactgatat aacagaattt
     1861 aagaaaatac aagaagagtt agattcaaaa agatttgaaa tacaggctat caatgatagt
     1921 attgttggtg gaatagtgat aactgagata aatgatgatt ttgatagtat atatttaaat
     1981 gaaggttatt taaatatgat tggatataca cgcaaacaac tgaaagatga gctaaataat
     2041 aaattaatca aacttatttt taaagatgat agaacagaat taattaatag tataaacaaa
     2101 caactcaaat taagtgatac atttgcatct gaacaaagag ttgaaaaaag ggatggcact
     2161 attatatgga ttttattaaa aggaagaaag atgatttatg ataatggaaa atctgtactt
     2221 tgttgtgtta ttgtagatat tacttctgct aaattattag agcaagaact taaaaagagt
     2281 gaagaacgtc atgaaatcat tctaaagcag acgcaaaata ttataataga atgggatata
     2341 gaaaaagata aaatgaatat atcacctaat tggtataata agtttaaata tgaccctata
     2401 actgaaaacg caacaaaaaa tataattaaa tcagtagata tatatcatga tgatatggat
     2461 aagtttattt cattaattaa acttacttca caaggtagtg agtatgaaga gtgtgaaatt
     2521 agacttagaa ctagagatac tatttacgct tggtttagta taaaggcaac aactttattt
     2581 gataataatg gtaaacctta taaagtagta ggaagtttga ctgatattga tgaatcaaaa
     2641 cgagaagctg aaaagttgaa agaaagggca gaaaaagacc ctcttacaga tttgtataat
     2701 aaaaaagcat cacaatcact tatagaagag tacataacat tggataaaaa aagaaaaggt
     2761 gctttaatga ttattgatat tgatgacttt aaaggaataa atgataattt aggacattta
     2821 tatggggatg ctgtattaag tgaagttgca aatgacctaa taaatctatt tagagcaacc
     2881 gatatagtag gcagaattgg tggagatgag tttattgtat ttataaaaga tatttctaaa
     2941 acaagagata ttataaataa agcagaagaa ttggtaaaaa catttgaaag gtctttttta
     3001 ggtaaaggac gaaattataa aatatcatta agtgtaggaa ttgctaagtt tccacaagat
     3061 ggtatggact atatgacact atttagaaat gcggatatag ctctttactc agccaaaggg
     3121 aagggcaaga attgttatac attgtatgat aaaaaaattg ataaagtcca atatataact
     3181 aatacaagaa atgcaggaga tagtgataat gtagagatgt cttttaaaga acatatcaca
     3241 aattatatat ttgatatact atatgaatca agtgatatag aagtggcagt aaatttaatt
     3301 ctaagtattg taggtaaaca ctttaatgtg agtcgcatgt atatatttga aaattcagat
     3361 gataatcgag tatgtaaaaa tacatttgaa tggtgtaacg aaggtataac ctcggaaatt
     3421 aataatctta aaaatatttg ttacaaggat ttaggcaact atcttttaaa ttttaatgca
     3481 gatgggttat tttattgtaa tgatattgaa aatttaggta gtgatgtgaa aaaagtactt
     3541 aagccacaag gcatacgttc aatattacaa tgtaaaattt tagagggtaa tgaatttaga
     3601 gggttcatag gatttgatga atgttcatat aatcgtatgt ggactaatga tgaagtgaac
     3661 actttgacat ttatatctaa aacgctcaat atgtttttat taaaaatgcg tattcagagt
     3721 aagctagaag agtctttaac aattcaaaaa tctgtgatgg ataatatgga tatatgggca
     3781 tatgttgttg acaggaatac ctatgaatta ctatttatca ataaaaaaat gcttgagtca
     3841 gcaccaaaca ctcatgtagg tgatttatgt tataaagcga tatggaataa tcaagataaa
     3901 ccttgtaaaa aatgccctat gatatattta aaagatggtg atgatactta taaatcattc
     3961 atgcataata aaaagtttga ggtacaggca aatataacta caactacttt gaaatggttt
     4021 gatggtaaag atgcatgttt aatatttgga agtgaaataa ctagtattga agctgaagaa
     4081 aatacttaaa atactgtgtt atatatatac ttaaaatcgc aatttgaatt tattagactt
     4141 aaaatgtcta tataaatcat ttaagttgtg gttttaagta ttttttatga tataattaag
     4201 cgttaagaac aaaattaaaa gatacttttg tataaaaaaa tatttgttaa atatatattg
     4261 agcaggtgat aatatggaaa ataagttttt accaataagc aaacaagata tgatagatag
     4321 aggatgggaa gaattagatt ttgttctagt tacaggagat gcttatgtgg accatcatag
     4381 ttttggaaca gcaataatat ctagagtttt agaaagtgct gggtataaag ttggaataat
     4441 agctcaacca gactggagaa ctacagatga ttttatgaag ctaggaaaac ctagacttgc
     4501 atttctagtt aatgcaggaa atatggattc aatggtcaat cattattctg taagtaaaaa
     4561 gcatagagaa aaggatatgt attctccagg tgggaaaatg ggttatagac cagatagagc
     4621 tactatagta tattgcaata aaataagaga agcatacagt gatgttgcaa tagtaatagg
     4681 tggaattgaa gcgagcttaa gaagatttgc acactatgat tattggagtg ataaggttag
     4741 aaagtctatg ttaatagata gtggagcaga tttattagtt tatggtatga gtgaaaagca
     4801 gattgtagaa gtagccaatg cacttaatga tggatatgac ccaaaataca ttagacatat
     4861 tgatgggaca tgctatattt cagatacatt ggaagaaata tatgataaat atatattaat
     4921 tccatcatat aaagaaattt gtgaagataa aatgaagtat gtagaagctt ttaaaattca
     4981 atatgatgag caagacccat ttagaggtaa tataattgtt caacctcatg gaagtaagta
     5041 tctggttcaa aataagccag aaaaaccact tagtagagaa gaactagatg aagtatatgg
     5101 attaccatat cagaagacat accatcctgt gtatgaaaaa tttggaggaa taccagctat
     5161 agaagaagta aaatttagta tagtaagttc aagaggctgt tttggtagtt gttcattttg
     5221 tgcaataaca tttcatcaag gtagagcagt gcaaagtaga agtgaaaaat caattattga
     5281 tgaagcaata ggaataacaa atttagatga ttttaaaggg tatatacatg atgttggagg
     5341 tccaacagct aattttagaa gaccagcatg taaaaaacaa attactaaag gtgcttgtaa
     5401 aaatagacaa tgcttatctc catcaccatg taaaaattta gatgcagacc acagtgaata
     5461 cttacattta cttagagctg taagaaaatt acctaaaata aaaaaggtgt ttgtgcgttc
     5521 tggaattaga tacgattatg taatggcaga taagaacaat aaatttctta gagagttgat
     5581 agagcatcat gtaagtggtc agttaaaggt agccccagaa catatatcag aagaagtttt
     5641 aaagtatatg caaaaaccag caggaaaaac ttatgataaa tttagacaga aattttttgc
     5701 tataaatgaa gaattaggta aaaagcagta tttgataccc tatttaatgt ctagtcaccc
     5761 tggttctacc ttaaatagcg ctatagagtt agcagagtat ttaagagata ctcactacca
     5821 accagagcag gttcaggatt tttatccaac tccagggact ctatctacaa ccatgtttta
     5881 tacaggtatt gacccgctta caatgaagcc agtttatgtt cctaaatcaa aacgtgacaa
     5941 ggcaatgcaa agggctttat tacaatatag agcacctaga aattatgatt tggtatactc
     6001 tgcgttagtt gaagcaggaa gagaagactt aattggtttt ggacatagat gtcttataaa
     6061 gccaaaaaat gaaaagcctt attttaatag aaataattct aaaaaaaatg tatctaaagg
     6121 aacaaataaa aacaaaaaaa ctaatacaaa taacagaagt aataaaaatc aacaaaaaag
     6181 ttcaactaaa aaaaagaaaa aacattaaag tataaataaa aatacttaaa aaagaaggat
     6241 tattcgataa attaacagaa attagtataa ataaatataa ttattttaat gacgttacct
     6301 ttgggaggaa gagatgaaaa agtatatttc tataatatta ttagtggtgt tgactatggt
     6361 tgttttggta ggatgttcac caggaaaaga taaccctaaa aataaggagc tatcagttgt
     6421 caaacaagta aaagtagctg ttccagatgg actaccagca gtggcaatag ctaaacttgc
     6481 aaatgaaaat ccagagatta aagaaggata tgaaacagtg tatagcattg aaaagacacc
     6541 tgaagcgatt tccactagag tgatgaaaaa agatgcagat atagctatag ttccttcaaa
     6601 catggctgca atagcgtata ataaaacttc atcatataat attgttggaa ctgtaggaat
     6661 gggttcactt tatttggtgt caacagaaaa tataaaagat tactctgatt tagttggtaa
     6721 agaagttgga tgtactggaa aaggacttac tccagacata actataaaaa gtcttttgtt
     6781 acaaaaggat ataaattatt cagatataaa atttaattat gtaaattcag caagtgaact
     6841 ggtaccatta ttggctactg gtaaagttaa aacaggtatt gttccagaac ctgcacttag
     6901 tgctttgatg tctaaaaatc cagacataaa aattataaaa agtttaaatg actcatggaa
     6961 ggaagtaagt ggttcaaaag atggttatcc tcaatctact ttaattgtaa agtctgaatt
     7021 tttaagagat aataaagatt ttgttgattc ttttgtaggt caattatcaa atagtataga
     7081 ttgggcaaac aaaaatcctg aagaattagg tgcatattct gaaaaaataa aaatatctac
     7141 tgaatcaaag ataataggaa aatctcttga aagagctaat ttgaaatata ttccagttaa
     7201 ggacatgatt aaagattata aaaattacta tgagaaatta gctaattttg atgataagac
     7261 attaggaggc aaagtaccag atgaagcaat atactttgtt gagaaataaa aaaaataaag
     7321 aacaatttat agataaaatg gaggtaacac tttcttgtgt tatcctctta tttttatggc
     7381 aaattattgc tttaaaaatt gataatgata tttttttacc aacagtaggt caagtgttta
     7441 atagtataaa agagattatg ctaaattcta gtttctatat agatatactt tactctatgg
     7501 ggagatgttt ttttagtttt ttactggcta tgatttttgc tattatactt tcaatagtgt
     7561 cttatttaaa tagatttttt agaaacttat taaagccaat caatgctttg acacgttcaa
     7621 taccaactat gatattggta gtacttgcac ttatttggtt tgaaaaggat agtacccctt
     7681 ttatagttgg atttactata gtttttccta tattgtatga taacgttttg ggcgctatat
     7741 tgaatataga caagaactta ttagaaatgg ctaatgtgta taaaataagg tttatagata
     7801 aggtgttaaa aatttattta ccagccataa aatttcaaat tatatctata ctagtatcaa
     7861 cattctcttt aagtttgaaa gttgctatag caggagaggt atatagtcaa cctacatatg
     7921 gtattggaac tatgatacag actgaaaaaa ttaactttaa cacatctgga atatttgcat
     7981 ggataattat tgttctttta atatctgcta ttttacaaat agctcaaaag tttttagcaa
     8041 ggagagcttt tttatggaaa agataaagtt gagtattgaa aatataaata aaagatatga
     8101 tagtcgtatt atatttagag attttaatat tgatttttat gtaaatgagg taaattgtat
     8161 ccttggaaaa tctggttgtg gaaaaactac actcttgaac ataataagtg ggattattaa
     8221 aaatgacacc aacaatctaa atatcaaaga aaatttaaat agagtaggaa ataagcttga
     8281 agctagttac atatttcaag atgatagact tatagactgg ttaacagtag aagaaaatat
     8341 aaagatagtt gtaaataaat actataataa aactcaatta aataaaattt gtgatgaata
     8401 cttggaattg gttggaatta gtgattataa aaaattctat cctcaaatgc ttagtggagg
     8461 tattagacaa agagtaaata ttgctagagc ttttatatat ccatcaaaaa acattataat
     8521 ggatgaacct tttaaatcta ttgatgcaaa gaatacacag cttataatgg ataatttcag
     8581 aaatatatta agaaaagaaa agagaactgt gttatttgta actcataata ttgaggaagc
     8641 attgttttta gcagatagga tatttatttt aggagactct ccaattagaa taaaaaaaat
     8701 attaaaaaat tctaaggaat tggaaaaaaa tgaagttttg aagttgatat agagttataa
     8761 taatactgtc gttttaaaat atatttggtt aaaatctata agactatatt aaaatagtac
     8821 agaatattca aaactgtgta taatgttcgt attttataaa aaggaagtga atgtatggtt
     8881 aagactaatg ctatgagaat attagattca aataagattg attataaagt aatgtcttat
     8941 gaagtgaaaa gtgaacatgt ggatggtgtt gaggtggcac atgatatagg aagagatgta
     9001 aatgaagtgt ataagacatt agtaactcaa ggagttagta agaatatata tgtatatgta
     9061 ataccagtac atgaaaactt agacttaaaa aaggctgcaa aagtagctaa agagaaaagt
     9121 gtagagatga ttcatgtaaa agatataaat aagcttacag gttatattag aggcggatgt
     9181 tctcctatag gtatgaaaaa gctttataaa acttttgtaa atgaaagtgc aaagaattta
     9241 gatacaatta ttgtgagtgc aggaaaaata ggatatcaaa tagaactatc tccatttgat
     9301 ttacaaagac ttataaaagt agaatttgta gatgtaataa aaaaataaga attacatata
     9361 aaatatagag cttatattct caataatagg tgttgtaata ttaataaaat gaataataat
     9421 tttaaaataa atattcagtt acttatgtgt aattggtata aaaatattga gaaattttaa
     9481 aaacaatggt aaagtgtaag tataatcacg aatatttttt ataaagtaat ggagggatgt
     9541 tcgcttgaga aaaataaaat ctgaacaaat cgtcgagcag gtaaaaaaac tttgtataga
     9601 agcgagccta tatttaggag aagatgtttt aagttgtata aaagaaaaag cgaaaagcga
     9661 aaagagtgaa gttggaaaaa atatattgaa tatattagtt gaaaatgctg aaatagctaa
     9721 agaaaaaaat ataccaatat gccaagatac tggtatggct gtattttttg ttgaaatagg
     9781 acaagaagtt ttaattgaag gagacacact tactgatgct ataaatgagg gagtaagaca
     9841 agggtatgaa gaaggctact taagaaaatc tgttgtaagt ccaattaaca gagtaaatac
     9901 aaaagataat acacctgctg ttattcatta tgatatggta aaaggtgata aaattaaaat
     9961 agaatttgca gctaagggat ttggaagcga aaatatgagt aagatgaaaa tgttaaagcc
    10021 atcagatgga ttggaaggaa ttaaaaaatt cataatagat acagtttcag aagctggacc
    10081 taacccatgc cctcctatgg taattggagt tggtataggt gggactgtgg acaaatgtgc
    10141 tcaaatagct aaaaaggcac tttttagaga actaggtgaa tttaataagg acgaaaatat
    10201 agcaaaactt gaaagtgaac tattaacagc tattaataaa cttggtatag gacctcaagg
    10261 cttaggtggt actacaacag ctttgggatt aaacatagag acttttccta cacacatagc
    10321 aggtctacct gttgttgtaa atataaactg ccatgcatca agacataaga aggtagtaat
    10381 ttaggaggaa aataaaatga taaaaataac tacacctgta aatgaaattg atatagcaaa
    10441 attaaattgt ggagatacaa ttagtttgag tggtatactt tatacagcaa gagatgctgc
    10501 tcataaaaga ttgatagatt gtataaataa aggtgaagaa ttaccatttg acgtatatgg
    10561 tcaaggaata tattatgtag gacctactcc tacaaagcca ggagaagtta taggtgctgc
    10621 aggtccaacg acgagttata gaatggatga tttgactata cctcttttag aaagaggtct
    10681 tagattgatg ataggcaagg gaaaaagaag tgatgaagtt atagaaggta tgcaaaaata
    10741 cggagcagtt tatcttgctg caataggagg agctggagcg tatatttcaa attcaataaa
    10801 atcatgtgaa attattgcat atgaagattt aggagctgaa gctataagaa agattgtagt
    10861 agaagacctt aagcttacag tagcaataga tagctatggg aataatatat atgagcaagg
    10921 tcgagcaatt tatgaatgta aataatgttt agaaataaat atgaatatca aaattaaaaa
    10981 tagttgtgta tattttaagg aggaaaattg atgtctaagg attatgcaaa attggcatta
    11041 gaaatgcatg aaatcaacaa agggaaagta agtgtagaaa gcaaggtaga agttaaaact
    11101 aaggatgatt tatcaacagc atatacacca ggggttgcag aaccttgttt aaaaatacat
    11161 gaaaatcaag atgatgttta tagatacact tctaaaggga atctagtagc agtagttagt
    11221 gatggaagtg ctgtacttgg attaggtaac attggtgctg aagcatctat acctgttatg
    11281 gaaggtaaag ctatattgtt taaacaattt gcagatgtag acgcttttcc aatatgttta
    11341 aagactaatg atgtagatga gatagttaaa tctgtagagt tgatggaacc tgtatttgga
    11401 ggtataaatt tggaagacat atcttctcca agatgttttg agatagaaga gagattaaaa
    11461 aaatctttaa gtatacctgt ttttcatgat gaccaacatg ggacagcaat aatagttgca
    11521 gcagccataa taaactcaat aaagttaatt gaaaataaaa aaatagaaga tttagaaata
    11581 gttataaacg gagcaggagc tgctggtata gccatagcaa agatactact taatatgaat
    11641 gttaaaaata taatcttatg tgatagaagt ggagcattgg aagctagtat agaaaatcta
    11701 aactatgttc aaaaagaaat gttaaaagta acaaatataa gaaatgaaaa aggaccttta
    11761 aaagatatta taaaaggaaa agatgtattt attggtgttt caggtccagg agctgttaca
    11821 aaggaaatgg ttgaaagtat gtctgagaag ccaataatat tggcaatggc aaatcctgct
    11881 ccagagataa tgccagaaga agcaaaatta ggtggagcat ttataatggg aacaggtcgt
    11941 tcagacttcc ctaatcaagt caataatgta ttggcttttc ctggaatatt tagaggagca
    12001 ttagatgtta aggctaaaga gataaatgaa gaaatgaaga tagctgctgc atatgcaatt
    12061 gctaatacaa taagtgatga ggaaatatgc ccagagtata tattaccaga tgtatttaat
    12121 aagaatgtag taaaaaatgt tgctaaggca gttaaagaag cagctataaa aactaaagta
    12181 aatagaatat aaatttttat atgctcatat attcttactt ttatatacat atctttatga
    12241 ttaaacgtgt tagaaaacgt agtaaattta tactatcaga ctttaccttt attacttaaa
    12301 atgatataat atgaatatta taaattaata aatttaaatt tattgaggtg caagagataa
    12361 tggaatataa agttaatgac caagaactta atgcttcaat gtttatccca ttcgttaaca
    12421 ggatatggcc aggagattat gatacggata aaacacagac tgcattatca aaaactttga
    12481 atataactgc ttatgatgat aaagtgcttg taggatgtct gcgagttctt tcagatggtt
    12541 attattttgg aacaattaca gaattgcttg ttcttccaga gtatcagaaa caaggcattg
    12601 gtagtaagct tttccagctt gctaaagaga atacaccaac tatgctatat tttggtgcac
    12661 agccaggagt agaagagttc tatgaaaaaa atggatgcca aagaagtctt caatcttaca
    12721 taataaaaaa gaaagatagt tattaaagtt ccagctttca ataatcttaa aagtacaata
    12781 ttttagtgga atcatttcat tttttattac taatatgaca taaacatgtc atattagtat
    12841 tgatatactt ttaatatggt taagagccaa agaaaaatat aaggagtgat ttaaaatgga
    12901 agaaactaaa ttttgccaat gctgtgcaat gccaatggga caaactgatg aattatatgg
    12961 gacaaataaa gatggaagta aaagtactga ttattgtagt tattgtttta aagatggtga
    13021 gtttacagct gatataagta tggaagagat gatagaagtt tgtatccctc atatgcttca
    13081 aagtaataag gatatgactg aagatgaagc aagaacaatg atgaacaatt tctttccaac
    13141 attaaaacgc tggaaataat tttatgcaaa attagttata tgcaaaacat gaaatatggt
    13201 gttgagaata aggggtggtg tgctttatag cataccacct gttttgttac ttgctagttc
    13261 aggtattctt gtatgcttaa atatgttctc atctctcttt ctattttaaa ttgtaaaaat
    13321 ctaaaatatg ttatttatat tctacttctt tatccatgtc tttttctacc acaagtcaaa
    13381 tccaactaca aaatcatcat tttcaattca ctgtttaata gaattaacag ataaagagtt
    13441 ataaaatttt ctatttttta tataaaataa tacaggtcaa gctgacttct agctacttgg
    13501 gatagtgtat atatttatga tagttatagg agtaatacca atcattagac tgttgatttg
    13561 tgtagtcttt tgtaaaaaca tttgttttaa tatattgagt ttttgaaaaa gtaaaattat
    13621 aattataata aattaagtgt aataatgtat tagaagtaaa aataattaaa atagaggtga
    13681 ttaaatgagt agtatattgt tagttgaaga tgatttaagc ttgatatatg gattagagtt
    13741 ctctatacaa aaaagtggat ttagtataga catagcaagg acagtaaaag aagctatgca
    13801 aatgtatgag gaaaaaaatt atgacttatt gcttttagat gtatccctac ctgatggtaa
    13861 tggatttcag atttgcgaaa aagtacgaga ggtttctaat atacccatta tatttttgac
    13921 tgcttcagat gaagaggtaa atgtagtgat gggtcttgat atgggtggag acgattatat
    13981 aacaaaacct tttaaattaa atgaattaat ttcaagaata aaagcattac ttagacgtta
    14041 taatgttgca agtaatgtga atgaattaag ttcaaataat attactatta aattacttga
    14101 aaatcgtgtt tttaaaggta aatttgaggt ggaacttacc acagcagaat ataaattatt
    14161 gtgcttattt atgaaaaata aaaatattgt acttactaga aaaaacatat tggacaagtt
    14221 atgggatgga aatggtagct ttatagatga caatacatta tcagtatatg taagacgatt
    14281 gagaaataaa attgaagata atcctgaaaa tcctaagttt ctattgaccg ttagaagaat
    14341 gggttataag tggaatgtaa ttaagtgagg gtattaatat gaagatattt tctaataaag
    14401 atataaaaat atttttttta atgcttatat ctattttagt aatgtttatt atagtagggc
    14461 aaatagtggt tataagtata actaacaatt ataaaaaaac gttgatagag catgattata
    14521 atatagctgg atacttatat agtataggtg ttgataaatc taaaatacca tcagcatttg
    14581 ttgcacatga agataaatat attttaaaag gaaaagaaat actagataca gctggatatg
    14641 gatttgatat agatgttagc tttacaccaa gtgtaaaatt gtttcaaagt aaatatataa
    14701 aaactatgtc tatctattta ttagtatttt ttactatgat tatttttata ctatatatgt
    14761 atttttttag gcaacaaagt aagatagaag aggctagtta taaaattgat ttatttatga
    14821 atggaaatat aaatgaaaga ctaaatacat atgaagaagg tagcttatca aaattattta
    14881 gttcaataga tgttatgtcg acatctttaa atacacatat attaaaagaa aaacagagca
    14941 gagaatttct taaaaataca atttcggata tatcgcatca attaaaaact ccacttacag
    15001 ctttaataat gtacaatcag atactacaag aagaaagtca taatagtgat gtcgttgaag
    15061 cttttgtaca aaaaagtcaa agtgaactag aacgcattga aacattgatt aagaatttat
    15121 taaagattac aaagatagat tcaaaaacaa taattctcaa taaaaataac gttaatctta
    15181 aaaatttaat tgtcaaaatt ttgtatagct ttgaaactag ggcagaaaaa gaaggaaagg
    15241 ttatttctgt aaatggttca gaaaatacat tgttgttctg cgattatgaa tggatgtcag
    15301 aggctttaag taatcttata aagaatgcat tagataatac aaaagagaat gataaaataa
    15361 cgatagagtg gtttgagacg actataacca ccattgtttc agttaaagat actggaaatg
    15421 gaatccatgt tgaggatatt catcatatat ttaaaaggtt ttatagaagt aagttttcaa
    15481 aagataatca aggtttagga ctgggattac cactagttaa atctattgtg gaacaacata
    15541 atggtactat ttctgtagaa agtaaactat cacaaggaag tatattcacc ataagttttt
    15601 taaagcttac taatatgtaa gataaaatac atcttgcagt aaggtatata gattattatt
    15661 atattaatca attagaaagg tgagagagaa aatggatatt cttgtggtta ataatttatg
    15721 taaaacatat ggaaatggat atacaaaggt agaggcattg aaagatgtat cctttacagt
    15781 ttcaaaagga gagtttgtga ctgtagttgg agaatctggt tcaggaaaga gtacactatt
    15841 aaatctctta ggaggtcttg atactccaac atcagggaga atatttattg atggtaaaga
    15901 aatatgttca atgaaagagg aaaaacagac tatttttaga cgtcgtaaca tagggtttat
    15961 atttcaggca tataacctta taccagaact aactgtggag caaaatataa tattcccatt
    16021 gttattagat tataaaaaac cagatactat ttacttagat gaagtattga ctattttagg
    16081 tcttaaagat aggaaaaatc atcttccaaa tcaattatct ggtggtcaac aacagagagt
    16141 tgcaattgga agggctttga ttacaaagcc aagtattata ctggcagatg agccaacggg
    16201 aaatcttgac agtaaaaata gtaatgatgt aatttcactt ttaaaagtga ctgcagaaaa
    16261 gtataatcaa accattatta tgataactca taatacgaat atagcttcta cagcagatag
    16321 aatactacag gtttctgatg gtgtgcttac agacctgggg gtgaatagag aatgagaagc
    16381 tacttagaat tagtaccaca atatgcaaaa gtacataaga aaaaaaatag aactacaatt
    16441 ttatgtatta ttattgcagt atgcttagtt actgctatct ttggacttgc agatatggct
    16501 atacaatgtc aaaagataca atttattaaa gatgatggaa attggcacat agtaattaga
    16561 aatattgatg aaaagtttat atcagaaata aatagtagag tagatatagc tcaccatggt
    16621 agggggcaag tcgttgacaa tggagagttt aaatcaaagg agattttgat acaaggttta
    16681 gatgaagaag tagcaaagca atttaactta gaaataataa ggggtcgtta tcctattggg
    16741 gagaatgaga ctttaataga tgaggatgca gttaaaagct ttaatctaaa aataggagat
    16801 tttatcaaaa taaaattagc agatggtaga aataaaaatt ataagatagt aggtgaatat
    16861 tcatcaatat caagcttaag agctacagat aaacatggat taatactttc aatattgggt
    16921 acacaagcta ttaaaaaatc agaaacgtac gattattatt atattacatt taaagaaaag
    16981 gttaatatta gaaaagccac ttctgatatt aaaaatacct tcaatttaag tgataaacaa
    17041 attggtaaaa atgaaagatt actttctatt ataggtcaag gtgactctga cttttctaaa
    17101 ccattgtatt taacagcagg attacttttt gtacttgttt tagtagcaag tattatcatg
    17161 atatataata gttttaacat gagtgtaatt gagagagtta aattttttgg tctgttacgt
    17221 tgtctagggt catcaaaaca tcaagttaaa aaatttgttg ttttagaaag tttaatttta
    17281 agtataaaag ctattccaat aggcttagta ttaggaagtc ttgtaactat tatatcttct
    17341 atatttctta agtatgttaa tgagttattc agctcaatgc caattttaaa agttagtttt
    17401 attggtattg catttggaat aattgtaggg tttttaactg taatattatc agctattatt
    17461 ccagcaaaaa aagcttcccg tgtatctcca cttagcgctg tacgtgggaa tctaatgtcc
    17521 aaagaaattt taaataacgg gaagacaagt aaagttgcaa atataggaag ttttaaaatt
    17581 gagacttcta tgggattaaa tcatgctttt tcaagaaaga aatcttttgt acttatgact
    17641 tcttcatttg ccattagtat tatactattt ttaggtttta gtgtgcttat agaccttatg
    17701 tatcaaggac ttaagcctat gaagccagaa gctcctcata tatctattgt tgaccctaat
    17761 tttaagtctg ttttaactaa ggaagatatt aatgaaataa gcaatatcaa aggagtaaag
    17821 aaagtataca gtagaatgat gacggatata gaagctacat ataatgggaa gaagacaaaa
    17881 tcacaattaa tttcttatga aaagtatcaa tttaattggg caaaaaaata tctattaaaa
    17941 ggagaagtta atgaaaataa actacagtca gaaaaccttg cattagtaga agaggggcat
    18001 ggatttgata taggagataa gatatatatt aaaaatggaa atgctgaaaa agagttagag
    18061 gtaggaggta tactttcctc aattccattt gaggctaaag ataaatatat tggaaatatt
    18121 ataacatctg aaaatacttt tacgaatata acaggtataa agggttatat aataatggat
    18181 attcaggttg ataaagatac tcctggagat ttaattgaaa atataagacg tgtagtagat
    18241 gataacttag aggtcagaga taaaagacaa agtaacttag aggttaaaaa ttcgttttat
    18301 actatggcaa catttgtata tggatttgtg tttattattg cgattataag catgtttaat
    18361 atcataaata gcatgaatat aagtgttaca agtagaaaga attactatgg tgttatgcgt
    18421 gcagttggca tatccaacag acaattaaga agaatggtaa tagtagaggc aggttcatat
    18481 gcagtgagtg gttgtttatt aggtagtata ctaggattaa tattacatag gtatatgttt
    18541 atatcgttag taagctcaat gtttcattta gagtggcaag taccttttaa tttactactt
    18601 atcactgtac taactatgat aataataact ttattatcag taagaaaacc aatcaaaaag
    18661 atttgtgaat tagatataat agactcaata aatgcacaat aaatataggt attgatttaa
    18721 attaaaaaaa cgaagtaaaa atgaaataga aaatattttt catttt
//
LOCUS       DAEPIN010000072        18488 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.072, whole genome shotgun sequence.
ACCESSION   DAEPIN010000072 DAEPIN010000000
VERSION     DAEPIN010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18488
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.072"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            <1..651
                     /locus_tag="KR250_002964"
     CDS             <1..651
                     /locus_tag="KR250_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0068862.1"
                     /translation="NGFVLGFICTYITTYKKSYEKENMYLSLFSNSIRTFILGYVLVL
                     VILLVLTISDSSYLNELDMSSYSNGLNLFTILPQIASYMWAFANGISVTIINSTVSMF
                     TLSSSSLFGDTKLMFYAMGALSMLILLLNGYKLRFKYNTDSIRPIIVFSIYYAFLMGI
                     LALFSTFILDSNINFFNTTNYGTTLIMQFKVLQAIVISFVYSFVISLIGYKLNSAD"
     gene            673..1164
                     /locus_tag="KR250_002965"
     CDS             673..1164
                     /locus_tag="KR250_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBH0068863.1"
                     /translation="MEGNTNNKVDDKFSKVKKKFGSGKFKVELYKEKSKLNREIDDIQ
                     EKKAKIFLEMGILTYQKIREDSINDNSFDKFCDELLELDKLIYEKNMEINEMEMAESN
                     VTCECGYVGNVNDKYCAECGKKFELENECDDFIICGYCESEIDTEAEFCPCCGRKIIM
                     DLE"
     gene            1436..2107
                     /locus_tag="KR250_002966"
     CDS             1436..2107
                     /locus_tag="KR250_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HBH0068864.1"
                     /translation="MLDKVKGRLIVSCQALENEPLHSPFIMGRMAKAAMEGGAVGIRA
                     QGVEDIIEIKKVTGLPVIGIIKRNYEDSDIYITPTKKEVDELLTTGCEMIALDATNRV
                     RPNNEDLKELIKYIKENGVLVMADISNYDEAIKAQEYGVDCVSTTLSGYTPYTKTLEG
                     PDFVLMKRLVKDLEIPVIAEGKVNTPQDLKKVFELGVHSSVVGSAITRPQLITEKFVK
                     AIEIN"
     gene            2192..3058
                     /locus_tag="KR250_002967"
     CDS             2192..3058
                     /locus_tag="KR250_002967"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HBH0068865.1"
                     /translation="MKGIYSALLVSFDKEGNINEKGLRQIIRHNIDVCKVDGLYVGGS
                     TGENFMLSTDEKKRIFEIAKDEVKEEIKLIAQVGSVNLKEAVELAKFTTDLGYDAISA
                     VTPFYYKFDFEEIKHYYNTIINSVDNRLIIYSIPFLTGVDMSLDQFGELFENEKIIGV
                     KFTAADFYLLERMRKTFPNKLIFAGFDEMMLPATVLGVDGAIGSTFNVNGVRARQIFE
                     LTKNEKISEALEVQHVTNDLITDILGNGLYQTIKLLLEEQGVEAGYCRQPMKEATDEM
                     KSRAKEIYRKYF"
     gene            3091..4593
                     /locus_tag="KR250_002968"
     CDS             3091..4593
                     /locus_tag="KR250_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HBH0068866.1"
                     /translation="MVGFTWIDFAILVVYLLAVLFAGLLFSKKEMKGKEFFKGDGTIP
                     WWVTSVSIFATLLSPISFLSLAGNSYGGTWILWFAQLGMFIAIPLTIKFFLPLYSRLE
                     IDTAYEYLEMRYESKGLRVLGALMFIIYQIGRMSIIMYLPSIVLAGLTGIPVNVLIIV
                     MGVIAIIYSYTGGLKAVLWTDFIQGMVLIVGVTGTLFYLIASINGGFGTVMDTLTTGH
                     KFLASNEVMFDKNILSTSAFIIFVGAGLNTFSSYVSSQDIVQRFTTTTDIKQLNKMTY
                     GNGVLSMGLATVFYLIGTCLFIYYTQNPDLAQTVQQDQVFASYIAYELPVGITGILLA
                     AIYAASQSTLSTGLNSVATSWTLDIQTIITKDISMEKQTKIAQYISLGVGILSIAVAI
                     VLANGEIKSAYEWFNSFMGLVLGVLAGIFVLGAFCKKATKMGAYVGFIVSAILVVYLK
                     YRMPEVTSWAYSLITITTSVVVGTIVSAIEAKVKAKVSLPKAETTVYYEK"
     gene            4723..5175
                     /locus_tag="KR250_002969"
     CDS             4723..5175
                     /locus_tag="KR250_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HBH0068867.1"
                     /translation="MIFGNINHSKTYSTLHEDLLRCFEYAKDNSLIDYEKGTYTIDGD
                     DIFVNIVEYKTCEKEDRFWEAHKKYIDVHLMLDGCERIDINFIENLEQKIYEEKGDFL
                     PLDGKNNGYMELRKGDFLVCYPEDAHMTGIKVLEKENIKKAIFKVLVR"
     gene            5335..6138
                     /locus_tag="KR250_002970"
     CDS             5335..6138
                     /locus_tag="KR250_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0068868.1"
                     /translation="MENIDVFLLIGQSNARGLGNPKESIIPNENCFEYLSTDEIINMR
                     CELETSEGDGTIAPAFSNEWNKLTGNKVCFIHNAKDGSRIKNWNHDNNWFLNDTIEKF
                     NTGCTTLSKHYNIENKYVIWIQGESDAKYGSDVLYYKESLKKIAYRLKEECMIDKMFV
                     SLTGYWLGEDEYFIRTRRIAAAQESACSECDILCVGSKIAMQFHDRGLTIDDVHYTQE
                     GLNMLGEDLCKNIYKYHITKEKSVLSDTIDLSEARKYICELEKISKRFV"
     gene            6196..7083
                     /locus_tag="KR250_002971"
     CDS             6196..7083
                     /locus_tag="KR250_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBH0068869.1"
                     /translation="MKNYVCIDVGGTSIKYGFIREDGFIIDKSSLDTEAKEKGGEGIL
                     AKIKDIVKKYIEENEISGICISTAGMVDPVEGKILFALEELIPNYKDMQLKKEVEKEF
                     NIKCEVENDVNCAGLGEMWLGAGRGATSSICLTIGTGIGGCIIINNKLINGFSNSAGE
                     VGYMNVNGSSFQELASTSSLIKKVAKIKNLNENDLNGKIIFDLAKNNDEDCLKELDNM
                     IKSLAVGIANICYVINPEVVILGGGIMAQEKFLKPKIDKALKEVLIERVYKNTNIEFA
                     KRQNDAGMLGALYNFLNKI"
     gene            7102..7950
                     /locus_tag="KR250_002972"
     CDS             7102..7950
                     /locus_tag="KR250_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBH0068870.1"
                     /translation="MSILEQLESPTFKVTKSDKLLIAYIKENIDDVFYKPISQIAKES
                     NIGEATITRFVKKMNFNGLQDFKVTLAQEISTINKKNIINKNIQNDEPALDTAKKLLS
                     SNVTTLENTVEIINSKDVHDCARLIINAKKVYFIGIGYSGIIAQDSNYKFMRIGLNCV
                     SFDSSHTMIMMSSIMEEGDLIIAISHSGETEEIIKTVKLARANNAKIISITENKNSEL
                     KDISDVHLSYVSGETVLETGSISSKLAQFFIIDLVYTQVVKELSNEAIERKIKTTNAI
                     KLFKNE"
     gene            8013..8702
                     /locus_tag="KR250_002973"
     CDS             8013..8702
                     /locus_tag="KR250_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBH0068871.1"
                     /translation="MSKLKDYNIDKVIIFNNLSTKTKNQLKEKVKLKRLQKKEILFYE
                     KDSLDSVYILLEGKVSIFKISENGERKVIFILNSGEVINDITLDASKNSTVGCEAFEN
                     SIVAYYSIEEFLEIMQNDFQLTKDIISYMERRIRRLYRQLKNSISIKVDKKLAAKLYR
                     LSREFGVCCGEWTLLNVNITVTYLADMLGCKRETVSRMIKVLQKEELIKIDNKGFYVK
                     ETELSRYFKNN"
     gene            complement(9032..10222)
                     /locus_tag="KR250_002974"
     CDS             complement(9032..10222)
                     /locus_tag="KR250_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009271880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBH0068872.1"
                     /translation="MSNVKRKDSKNRNLRNGESQRKDGRYVYKYTDIYGKPQFIYAWK
                     LVPTDKTPAGKRDDISLREKEAQIKKDLDDGIDTAGGKMTVCQLYDKKNSQRKNIKRA
                     TEKGRQYLMNALKNDPLGMRAIDTVKQSDAKEWAIRMSERGYAYKTIDNYKRSLKASF
                     YMAIQDDCIRKNPFEFKLSDVLEDDTEPKVILTPEQEERLLAFMETDKIYSKYYDEVV
                     LLLETGLRISEFCGLTTHIDMQNRILNIDHQLLRDSEIGYYIDTPKTKNGKRKIPLTE
                     RAYQALQRILKNRVKAQPLVVGGYSNFLFLNREGLPKVAGNYVGMVRGLIKKYNKYHK
                     DKLPNITPHSFRHTYCTNMANKGMNPNTLQYLMGHANITMTLGYYAHGTFQSAKAELE
                     RLAC"
     gene            complement(10300..10503)
                     /locus_tag="KR250_002975"
     CDS             complement(10300..10503)
                     /locus_tag="KR250_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HBH0068873.1"
                     /translation="MNNTDVPIWEKYTLTIEEASKYFRIGEKKLRKLAEENIDAGWVI
                     VNGNRIQIKRKQFEKIIDTLDEI"
     gene            complement(10885..11133)
                     /locus_tag="KR250_002976"
     CDS             complement(10885..11133)
                     /locus_tag="KR250_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004613734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH0068874.1"
                     /translation="MKHTFKKPSYYLLSQAMDGDEKAIEKVLAFYDPYISKCCLRPLY
                     DEYGNVYIVVDMELKGLIREALIKMILGFDIALEIEEE"
     gene            complement(11137..11556)
                     /locus_tag="KR250_002977"
     CDS             complement(11137..11556)
                     /locus_tag="KR250_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005361151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBH0068875.1"
                     /translation="MKPSDFQKTVQCRFESCLKKVVRHVVKDYQQGLKRRKEKEISFC
                     ELPEIMVENLAVWDDYETDYTIFNVCGNDIRVYDDELAEALKQLSERNRETLLMYYFL
                     EMNNEEIAKKQNISRSGVFQNRHNSLALMRKLLKEKR"
     gene            11977..12336
                     /locus_tag="KR250_002978"
     CDS             11977..12336
                     /locus_tag="KR250_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0068876.1"
                     /translation="MRKTKETHTFDFRPLGLAIREAREKAGLSRNDLGDKVFYGERHI
                     ADIENIGSHPSFQLFHDLVTMFNISVDEYFYPAEKVAKSTARRQIETSLDLLSDNELK
                     IIQGTIDGILNSRENKK"
     gene            complement(12455..12643)
                     /locus_tag="KR250_002979"
     CDS             complement(12455..12643)
                     /locus_tag="KR250_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006863419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HBH0068877.1"
                     /translation="MKNEMWVLCPICNNKTRTKVREDTELINFPLFCPKCKTETLINI
                     KHGKVTIIKEPDAKTQSR"
     gene            complement(12665..12889)
                     /locus_tag="KR250_002980"
     CDS             complement(12665..12889)
                     /locus_tag="KR250_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0068878.1"
                     /translation="MIGIKKRDIKKALKAGAKAGGSSYADVLADIEATIDEVMNSTDP
                     EVQANFKKYFGNKRPTPEEYIYKITKKTRV"
     gene            complement(12913..13103)
                     /locus_tag="KR250_002981"
                     /pseudo
     CDS             complement(12913..13103)
                     /locus_tag="KR250_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003064779.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
     gene            complement(13257..13538)
                     /locus_tag="KR250_002982"
     CDS             complement(13257..13538)
                     /locus_tag="KR250_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBH0068879.1"
                     /translation="MLIVEFTEKEDAVFQQAVEWLSSKLAYTPASFNQQKKGFDIDPL
                     TRTFTNEEGTSIYLTAKEFDLLYFLSSHKGQVFTKEQIYEKVWGYDNFY"
     gene            complement(13532..14047)
                     /locus_tag="KR250_002983"
     CDS             complement(13532..14047)
                     /locus_tag="KR250_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016285890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBH0068880.1"
                     /translation="MKSKPVKVGLRTIKTVLSAFLCFLIDSLRASSVPFYAVIASILC
                     IQPDMENSLSVAKNRELSTIIGGILGMLFLLMERIFLPIQPEILRELTLSILLIPIIN
                     LSVLLKQKKGTFLTCVVFLCVTVTHEKDISPIWFAWNRIVDTSLGIGISLAVNSFPFH
                     KFKVGGTMGKY"
     gene            complement(14037..14537)
                     /locus_tag="KR250_002984"
     CDS             complement(14037..14537)
                     /locus_tag="KR250_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015393784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HBH0068881.1"
                     /translation="MYISSIGISNGIIEKKYGSHGVQFNENDVPTYSIPFKIENAPDK
                     TVSFAFILEDKDAYPVTGFIWVHWLGANLTRSELKENESQTAKDFIQGANSWTSILGN
                     QQTREQTSCYGGMAPPDKAHIYELHVFALDKLLDLKNGFYLNELYQEMEGHILEEAVL
                     KGLYEK"
     gene            complement(14625..14990)
                     /locus_tag="KR250_002985"
     CDS             complement(14625..14990)
                     /locus_tag="KR250_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009291267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0068882.1"
                     /translation="MKLRDEYTCPLELIHDMIKGKWKPIILWRLRLGATSLSKLENDI
                     DGITQKMLLEHLKELIDYGFVDKKTFSGYPLHVEYFLTEDMGNQILEALRIMQHIGIE
                     YLRKNGMENELTEKHVIPD"
     gene            complement(14974..15525)
                     /locus_tag="KR250_002986"
     CDS             complement(14974..15525)
                     /locus_tag="KR250_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBH0068883.1"
                     /translation="MKKALLIIDVQNDYFPNGKCQLYKPEIALNTIKDLLKIFRKQNL
                     PIIYIRHESIHGTFFNPNTNGVQIHNDIKPLDTETVIVKHYPNSFYETNLQNKLVEIE
                     VTKLVVCGMMTHMCIDTTIRAAKDYGYEITLISNGCATKELKWNGVNIPADTVQSIFM
                     ASLNQKFANVITSTEFLSNEITR"
     gene            complement(15615..15974)
                     /locus_tag="KR250_002987"
     CDS             complement(15615..15974)
                     /locus_tag="KR250_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0068884.1"
                     /translation="MKLRESYTCPLELVHDIIKGKWKTIIIFQLRNGKCSFSELHHSI
                     AGITQKMLLEQLRELKHFGLIDKKTYDGYPLKVEYFLTGQGEKMLSAIKIMQEIGIDY
                     MVEHGMTEILDEKGICY"
     gene            16112..16471
                     /locus_tag="KR250_002988"
     CDS             16112..16471
                     /locus_tag="KR250_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0068885.1"
                     /translation="MRKTKETHTFDFRPLGLAIREAREKAGLSRNDLGDKVFYGECHI
                     ADIENIGSHPSFQLFHDLVTMFNISVDEYFYPAEKVAKSTARRQIETSLDLLSDNELK
                     IIQGTIDGILNSRENKK"
     gene            complement(16590..16775)
                     /locus_tag="KR250_002989"
     CDS             complement(16590..16775)
                     /locus_tag="KR250_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011058338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HBH0068886.1"
                     /translation="MKDKWILCPVCGNKTRLKIRADTELKNFPLFCPKCKTETLINIK
                     HGKVTIIKEPDAKTQSR"
     gene            complement(16814..17101)
                     /locus_tag="KR250_002990"
     CDS             complement(16814..17101)
                     /locus_tag="KR250_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015574378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0068887.1"
                     /translation="MSTLIIGFIVAGVLLIAEYLLCTKFRSPLWGGIIPLLILAGTIY
                     VFASGRIPFERPYIFPFVVVNTLFFGDWGTGRDKYKKIKQTEMEKMKAKDI"
     gene            complement(17104..17400)
                     /locus_tag="KR250_002991"
     CDS             complement(17104..17400)
                     /locus_tag="KR250_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009460866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2089 family protein"
                     /protein_id="HBH0068888.1"
                     /translation="MELDKIPQWILALEQEDATFLKNFALKSGSLKEIAKMYGVSYPT
                     VRLRLDKLIQKIEMCDQQEEEPFQTFIKGLAVDSRIDVETAKIIIEKYKKEKEK"
     gene            complement(17489..18394)
                     /locus_tag="KR250_002992"
     CDS             complement(17489..18394)
                     /locus_tag="KR250_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBH0068889.1"
                     /translation="MFKKNKKQTENVKEKKVRTVKVGTHKKSVIALWVVLIASVSFGV
                     YKNFTAIDMHTVHETETIQLRLTDTNGIENFVKNFAKSYYTWNNSKEAIEARTQAISG
                     YLTKELQALNTDTIRKDIPVSSAVNGVQIWSVTQADENQFSVIFTVEQLITENGSTKT
                     VRSAYEVTVYVDGSGNMVLTKNPTITSIPVKSGYTPKAKESDGTVDAVTTDEINEFLT
                     TFFKLYPTATAKELSYYVNEGVLKPIGKDYVFSELVNPIYNRIDSQVTVSVAVKYLDQ
                     QTKATQVSQFDLVLEKSGSNWKIIK"
BASE COUNT         6314 a         2796 c         3140 g         6238 t
ORIGIN      
        1 aatggattcg tattagggtt tatatgtaca tacataacta cttataaaaa aagttatgaa
       61 aaagaaaata tgtatttatc tcttttttca aattctataa gaacattcat attaggttat
      121 gttttagtat tagtaatact attggtacta actatatctg atagtagtta cttaaatgaa
      181 ctagatatga gtagttattc aaatggatta aatttattta ctatactccc acaaatagct
      241 agttatatgt gggcttttgc aaatggaatt tcagtaacca taataaactc aacagtatca
      301 atgtttactt taagttcttc atctttattt ggagatacta aattaatgtt ttatgcaatg
      361 ggagcattgt ctatgttaat attattatta aatggataca agctaagatt taagtataat
      421 acagatagta taagacctat aatagtattt agtatatatt atgcattttt gatgggaata
      481 ttggccttat ttagtacatt tatactagat agtaatatta atttctttaa tacaactaat
      541 tatggaacaa ctttaataat gcaatttaaa gtcttacaag ctattgtaat ttcatttgta
      601 tatagttttg tgatatcttt aataggatat aagctaaatt cagctgatta aatatgattg
      661 gagggaattt ctgtggaagg aaatactaat aataaagtag atgataaatt ttctaaggtt
      721 aagaaaaagt ttggtagtgg aaaatttaaa gttgaattat ataaagaaaa atcaaaatta
      781 aatagagaga ttgatgatat tcaagaaaag aaagcaaaga tattcttgga aatgggaata
      841 ctgacatatc aaaaaattag agaagattct attaatgata atagttttga taaattctgt
      901 gatgagttac tagaacttga taagttaatt tatgaaaaaa atatggaaat taatgaaatg
      961 gaaatggcag aaagtaatgt aacttgtgag tgtggttatg ttggaaatgt aaatgataaa
     1021 tattgtgctg aatgtggtaa aaaatttgaa ttggaaaatg aatgtgatga ttttataatt
     1081 tgtggttatt gtgaaagtga gatagataca gaggctgaat tttgtccatg ctgtggaaga
     1141 aaaattataa tggatttaga ataatattgg gaaacctatg tatagtatac atgggttttt
     1201 ttattgaaat tatttaaatg gacttataac agtagttata taaaatgaaa ataaacacca
     1261 aaaaaatgaa ataaaatttc ttttaaatgt aaaaaacaag ctaatatata aaataaatca
     1321 gtaaaaatat agatgcgaga aaaaatatga aaaaatattg caaagaaaaa gttttcatgt
     1381 tattattaat acataaatca agaaaataaa tttcagaaac gaggtaattt aaaaaatgct
     1441 agataaggta aagggaagat taatagtatc atgccaagct ttagaaaatg aacctcttca
     1501 tagtcctttt ataatgggga gaatggctaa ggctgctatg gaaggtggag ctgttggaat
     1561 tagagctcaa ggagttgaag atattattga aataaaaaaa gttactggac ttcctgttat
     1621 aggtattata aagagaaact atgaagattc agatatatat ataacgccaa ctaaaaaaga
     1681 agttgatgaa cttttaacaa cagggtgtga aatgatagca ttagatgcta ccaatagagt
     1741 tagaccaaat aatgaagact taaaagaact tataaagtat ataaaagaaa acggtgttct
     1801 agtaatggca gatatttcaa attatgatga ggctatcaaa gcacaagagt acggtgtaga
     1861 ttgcgtttca actacactat ctggatatac tccatatact aaaacattag aaggtccaga
     1921 ttttgtatta atgaaaagat tagttaaaga tttagaaata cctgtaatag ctgaaggaaa
     1981 ggtaaatact cctcaagatt taaaaaaggt ctttgagttg ggtgttcatt catcagtagt
     2041 aggttcagca ataacaagac cacaattaat aacagaaaaa tttgttaaag caattgaaat
     2101 taattaaaaa ttaatttata aattaaaaaa ttattatatt taactatatt tgaaaattaa
     2161 aattggaggt aaagatatga gaacaacaga tatgaaggga atttattcag cattattagt
     2221 ttcatttgat aaggaaggaa atataaatga aaaagggtta agacaaataa ttagacacaa
     2281 tatagatgtt tgtaaagtag atggtcttta tgtaggagga agtacagggg aaaacttcat
     2341 gctttcaact gatgaaaaga aaagaatatt tgaaatagct aaagatgaag tgaaagaaga
     2401 aataaaatta atagctcaag ttggttcagt taatttaaaa gaagctgtag aacttgctaa
     2461 atttacaact gatttaggat atgatgcaat aagtgctgtt acaccttttt actataaatt
     2521 tgattttgaa gaaataaaac actattataa tacaataata aattctgtag ataatagatt
     2581 aataatatac tctataccat ttttaactgg tgttgatatg agtttagacc agtttggaga
     2641 attatttgaa aatgaaaaaa taataggtgt taagtttaca gcagcagatt tctacttatt
     2701 agagagaatg agaaaaacat tcccaaataa attaatattt gcaggatttg atgaaatgat
     2761 gttaccagct acagttttag gtgtagatgg ggcaattggt tctacattca atgtaaacgg
     2821 tgtcagagct agacaaatat tcgaattaac taagaatgaa aaaatatcag aagcacttga
     2881 agtacaacat gtgactaatg atttaataac agatatatta ggaaatggct tatatcaaac
     2941 aattaaatta ctacttgaag aacaaggtgt tgaggctgga tactgtagac aacctatgaa
     3001 agaagcaact gatgaaatga aatcaagagc aaaagaaata tacagaaagt atttttaata
     3061 aaatactttt agtatgggag gattagtcaa atggtaggat ttacttggat agattttgca
     3121 atactagtgg tttacttatt ggcagtttta tttgcaggat tgttattttc aaaaaaggaa
     3181 atgaagggaa aagagttctt caaaggtgat ggaactattc cgtggtgggt tacatcagta
     3241 tcaatatttg ccacattatt aagtccaata tcatttttat cattagcagg aaattcttat
     3301 ggtggtactt ggattttgtg gtttgcacaa ttaggtatgt ttatagcaat acctttgaca
     3361 ataaaatttt tcttacctct atatagtagg ttagaaatag atacagcata tgaatactta
     3421 gaaatgagat acgaaagtaa aggtcttaga gtacttggag ctttgatgtt tataatatat
     3481 caaataggac gtatgtctat tataatgtat cttccatcaa tagttctggc tggtcttaca
     3541 ggaatacctg taaatgtact gataatagtt atgggtgtaa tagcaataat ttactcatat
     3601 acaggtggat taaaggctgt cttgtggacc gattttatac aaggaatggt attaatagta
     3661 ggtgttacag ggacattgtt ctatttaata gcaagtataa atggtggttt tggaacagta
     3721 atggatactc ttacaactgg acacaagttc ttagcaagta atgaagttat gtttgataaa
     3781 aatatactat ctacaagtgc atttataata tttgttggag ctggtcttaa tactttttca
     3841 tcatatgtat cgagtcaaga tatagttcaa aggtttacta caacaactga tataaagcaa
     3901 cttaataaaa tgacatatgg aaatggtgtt ttatcaatgg gacttgcaac agtattttat
     3961 ctaattggaa cttgtttatt tatatactat actcaaaacc cagatttagc tcaaacagtt
     4021 caacaagacc aagtatttgc atcatatata gcttatgaat taccagtggg tataacagga
     4081 atactacttg cagcgatata tgcagcttct caatcaactt tatcgacagg gcttaattca
     4141 gttgctacaa gttggacttt agatatacaa actatcataa caaaagatat aagtatggaa
     4201 aagcaaacta agatagcaca atatatatca ttaggtgtag gtatactttc tatagctgtt
     4261 gcaatagtat tagcaaatgg agaaataaag tcagcatatg aatggttcaa tagttttatg
     4321 gggctagtac taggtgtttt agcaggaata tttgtacttg gtgcattttg taaaaaagca
     4381 actaaaatgg gagcatatgt tggattcata gtatcagcaa ttttagttgt ttatttaaaa
     4441 taccgtatgc cagaagtaac ttcttgggca tattctctaa taacaatcac gacttctgta
     4501 gtggtaggta caatagttag tgcaatagaa gctaaagtta aagcaaaagt atctttacca
     4561 aaagctgaga ctactgttta ttatgaaaaa taatatttaa atgataaatt gtcttataga
     4621 gtgattagat aaataattta gttactctat aagattgtat aatatacttt gtatacaaaa
     4681 gtataaaaat agactttata attatatagg gagatagtgc ttatgatatt tggaaatata
     4741 aatcatagta agacatattc aacattgcat gaagatttac ttagatgttt tgaatatgct
     4801 aaagataatt cattaataga ttatgaaaaa ggtacttata caatagatgg tgatgatatt
     4861 tttgtgaata ttgtagagta taaaacttgt gaaaaagaag atagattctg ggaagctcat
     4921 aagaagtata tagatgttca tttgatgctt gatggatgtg aacgaataga tataaatttt
     4981 atagaaaatt tagaacaaaa aatatatgaa gaaaagggcg atttcttgcc tcttgatggt
     5041 aaaaataatg gatacatgga acttagaaaa ggtgattttt tagtttgtta tccggaagat
     5101 gctcatatga caggaataaa agttttagaa aaagaaaata tcaaaaaagc tatttttaag
     5161 gttttggtta gataatgata atttatataa ccactagggc tgaaaatagt gatttaactt
     5221 tcagcgatta gtgagacttc gtaaatttta gttagattac tattttcagc cttagttttt
     5281 tatgagaaaa ttaaaaataa ttaaaagctt aaaattttgt aaaggagtta acttatggaa
     5341 aatatagatg tattcttatt aataggacaa agcaatgcta gaggattggg aaatccaaaa
     5401 gagagcatca ttccaaatga aaattgtttt gaatatttga gtactgatga aattataaat
     5461 atgagatgtg agctagagac ttcagaaggt gatggtacta tagcacctgc attttcaaat
     5521 gaatggaata agcttactgg aaataaagtt tgttttattc acaatgcaaa agatggttca
     5581 agaataaaaa actggaatca tgataataac tggtttctaa atgacactat agaaaaattt
     5641 aatacaggct gtactacctt aagtaaacat tataatatag aaaataaata cgttatttgg
     5701 attcaaggtg aaagtgatgc aaaatatggt agtgatgtat tgtactataa agaaagtcta
     5761 aaaaaaattg cgtataggct taaagaagaa tgtatgattg ataaaatgtt tgtaagttta
     5821 actggttatt ggcttggaga agatgaatat tttattagaa caagacgaat tgcagcagca
     5881 caggagtcgg cttgtagtga atgtgatata ttatgtgtag gaagcaaaat tgctatgcag
     5941 tttcatgaca gaggattaac tattgatgat gttcattaca ctcaagaagg tcttaatatg
     6001 ttaggagagg acttatgtaa aaatatttat aagtatcata taaccaaaga aaaaagtgta
     6061 ttaagtgata ctatagactt atcagaagca aggaaatata tctgtgaatt ggaaaagata
     6121 agtaaaagat ttgtgtaata taaaatttta aatagagttg ttaatttata aaaataattt
     6181 gtatggagat atgttatgaa aaattacgtt tgtatagatg ttggaggtac ttcaataaaa
     6241 tatggtttta ttagagaaga tggatttata attgataaat caagtcttga tactgaagca
     6301 aaggaaaaag gtggagaagg aatattagcc aaaataaaag atatagtaaa aaaatatata
     6361 gaggaaaatg aaataagtgg tatatgtata tcaactgcag gtatggttga cccagttgaa
     6421 ggtaaaatac tatttgcatt agaagaatta attccaaact ataaggacat gcaattaaaa
     6481 aaagaagtag aaaaagagtt taatataaaa tgtgaagttg aaaatgatgt taattgtgct
     6541 ggacttggtg aaatgtggct tggagcaggt agaggtgcaa catcttctat atgtttaaca
     6601 ataggtactg gaattggtgg atgtattata ataaataata aactgataaa tgggtttagt
     6661 aatagtgctg gtgaagtagg atatatgaat gtaaatggaa gttcttttca agaattggca
     6721 tctactagca gtctaattaa aaaagtagca aaaataaaaa atttgaatga aaatgactta
     6781 aatggaaaaa taatttttga tttagcaaag aataatgatg aagattgttt aaaagaactc
     6841 gataatatga taaaatcatt agcagttgga atcgcaaata tatgttatgt aataaatcca
     6901 gaagtggtaa ttttaggtgg tgggattatg gcacaagaaa agttcctaaa accaaaaata
     6961 gacaaagcat taaaggaggt tcttatagag agagtttaca aaaacactaa tatagaattt
     7021 gcaaagagac aaaatgatgc aggaatgctt ggagcattat ataatttttt aaacaaaata
     7081 taaagaatag aagggttttt aatgagtatt ttagaacaat tggaatcacc aacttttaaa
     7141 gtcacaaaat cagataaatt attaatagct tatataaaag aaaatataga tgatgtattt
     7201 tataaaccaa tatcacaaat agctaaagag agcaatattg gtgaagcaac tataactagg
     7261 tttgtaaaaa aaatgaattt taatggatta caggatttta aagttacttt agcacaagaa
     7321 atatcaacta tcaataaaaa aaatattata aataaaaata tacaaaatga tgagccagct
     7381 ttggatacag caaaaaaact tttaagttca aatgtaacca ctttagagaa cacagtagag
     7441 ataatcaata gtaaggatgt acatgactgc gcaaggctta taataaatgc aaagaaagtt
     7501 tacttcatag gtattggata ttctggtata atagcacagg attcaaacta taaatttatg
     7561 agaataggtc ttaattgtgt aagttttgac agtagtcata caatgataat gatgtcttcc
     7621 ataatggaag aaggagattt aataattgca atttcacatt ctggtgaaac agaggagatt
     7681 ataaaaactg taaagcttgc aagagccaat aatgctaaaa ttatatcaat tactgaaaat
     7741 aaaaattctg aattaaagga tatttcagat gtacacttat cttatgtatc gggagaaaca
     7801 gtactggaaa ccggttcaat atcttctaaa ttagcacaat ttttcattat tgatttagta
     7861 tatacacagg tagtaaaaga actttctaat gaagctattg aaaggaaaat taaaactaca
     7921 aatgctataa aattgtttaa aaatgaataa aataagaaat ttgtaatgtt ttatagagct
     7981 ctcttaaaaa tttaggaatg gagagatatt acatgtctaa attaaaagat tataatattg
     8041 ataaagtaat aatttttaat aatttaagta ctaaaacgaa gaatcaactt aaagaaaaag
     8101 ttaaactaaa aagattacaa aaaaaagaaa ttttatttta tgaaaaagat agcttagaca
     8161 gtgtttatat acttttagaa ggaaaggtat ctatttttaa gataagtgaa aatggagaga
     8221 gaaaagtaat tttcatatta aatagtgggg aagtcataaa tgatattaca cttgacgctt
     8281 ctaaaaactc tactgttggc tgtgaagcat ttgaaaattc aattgtagct tattatagta
     8341 ttgaagaatt tttagaaatt atgcaaaatg attttcaact tacaaaggat attatttctt
     8401 atatggaaag aagaataaga cgtttatata gacagttgaa aaattctata tctataaaag
     8461 tagataaaaa acttgcagca aaattatata ggttaagtag agaatttgga gtttgctgtg
     8521 gagaatggac tttactaaat gtaaatatta cagttacata tttagctgat atgcttggct
     8581 gtaaaaggga gactgtttcc agaatgataa aggtattgca aaaagaagaa cttattaaaa
     8641 ttgacaataa aggtttttat gttaaagaaa ctgaattatc aaggtatttt aaaaataatt
     8701 aattatgatt aagtaagagt attattagaa ttttaattaa ataaaaatat tattaagaga
     8761 atctatttat agattctttt ttattgtcta atttattaat ttttatttta ccctattgaa
     8821 ttgtataaaa aaacaaataa gattcttttt tattcaaaaa acttactaaa tttttgatta
     8881 tcttttcata agttggtaaa tgccgatata tcccgtattt gagggcttta tcggtatttt
     8941 ctttttggaa gataccttgc ataagctggc atttaccagc atgaaaatgc aaccaaaagt
     9001 agtaaatgag tagtaaatat acgttccgat attaacaagc cagtctttcc agttctgctt
     9061 ttgcagattg gaatgtaccg tgtgcgtagt agccaagcgt catggttatg ttagcgtgtc
     9121 ccatgagata ttgcagggtg ttcgggttca ttcccttgtt tgccatattc gtacaatagg
     9181 tatgtcggaa tgaatgtggt gtaatgtttg gtaacttgtc tttgtggtat ttgttatact
     9241 tcttaatcag tcctcgtacc atacccacat agtttccagc gactttaggc aatccctcac
     9301 ggtttaagaa taggaaattg ctgtaaccac caacaaccag cggttgagct tttactctgt
     9361 tctttagtat tctttggagt gcttgataag ccctttccgt caatggaatt tttcgttttc
     9421 cgttctttgt ttttggagtg tcaatatagt agccgatttc gctatctctt aatagctggt
     9481 ggtctatatt gagtattctg ttctgcatat caatatgcgt tgtcagtccg caaaattcag
     9541 aaatacgaag tcccgtttcc agaagaagca caacctcatc ataatacttg ctgtaaatct
     9601 tatccgtttc cataaaggca agcaggcgtt cttcctgctc tggcgtaaga ataactttcg
     9661 gctcggtatc atcttccaga acatcactta gcttaaattc aaacgggttc tttctgatac
     9721 agtcgtcttg tatcgccata taaaatgacg ctttcaagga acgcttatag ttgtcaatcg
     9781 ttttgtaggc atatcctctt tcactcattc tgatagccca ctctttagcg tccgactgtt
     9841 tgacggtatc aatcgctctc atacccaatg ggtcattttt cagagcgttc ataagatact
     9901 gtcgtccttt ttccgtagct cgcttgatgt tctttctttg gctgttcttc ttgtcgtaaa
     9961 gctggcagac tgtcatttta ccgcctgccg tgtcaatacc gtcatcaagg tcttttttta
    10021 tctgtgcttc tttttccctc aacgatatat catcacgttt tccagcaggc gttttgtctg
    10081 tcggtacaag tttccaagca taaataaact gtggctttcc gtatatatcg gtatatttat
    10141 aaacgtatct tccgtctttt cgctggctct ctccattacg caaattgcga tttttactgt
    10201 cttttcgttt cacattagac atagtgtaaa gctcctttcc gtcatggaat gagccgtgat
    10261 acgctatact tattatacca tatccacggc tctatgacat tagatttcgt ccaatgtatc
    10321 tatgattttt tcaaattgtt ttcgctttat ctgaatacgg ttgccgttca cgataaccca
    10381 gccagcgtca atattttctt cggctaattt acgcaacttc ttttctccaa tgcggaagta
    10441 tttggacgct tcttcaatcg tcagtgtata tttttcccag ataggcacat cagtattatt
    10501 cataatttat gcaccccctt tactgtgtgt cagtctataa gttatataaa caggcagacg
    10561 gctttggaaa gctccattag tatttcaact attcccattc ttgtgggcag aaccgcacca
    10621 tgcggaagta tcattatgct gtaagggtag gtcatggcaa aacgactttt ccaacggtcg
    10681 ctctcggatc actgcgtggg tcgctcgctt tcttttggaa aggtcatggc gtacagttcc
    10741 cgtggctctc tatccttaca aacgtatctg tctgctttat tcagttttca aagagctgtg
    10801 gcgaaagcgg tatgctttca tgtaaaagca ggagcagagc aggaaagagg ggattgaaca
    10861 aatcatgctc tgcgttactg ctggttattc ttcttcaatt tctaaggcaa tatcaaaccc
    10921 taaaatcatt ttgataagtg cttctcggat aagtcctttt aactccatat ctacaacgat
    10981 ataaacatta ccgtattcat catagagtgg acgcaaacag cacttcgata tgtaagggtc
    11041 ataaaatgca agtacctttt caatcgcttt ttcgtccccg tccatagctt gtgaaagcaa
    11101 gtagtaagac ggcttcttaa atgtgtgttt catttgctat cgtttctcct ttaatagttt
    11161 tctcataagt gccaatgaat tgtggcggtt ctgaaaaact ccgcttctgg aaatgttttg
    11221 ctttttggcg atttcttcat tgttcatttc caaaaagtaa tacatcaaca aagtttctcg
    11281 gttacgttcg gataactgtt tcaaggcttc tgccaattca tcatcataga cacgaatatc
    11341 attaccgcat acattgaaaa tcgtatagtc tgtttcgtag tcgtcccata cagccaagtt
    11401 ttcaaccatg atttctggaa gttcacaaaa ggaaatttct ttttcttttc ttcgcttcaa
    11461 tccctgctga tagtccttga caacatgacg gacaactttc ttcaaacagc tttcaaaacg
    11521 gcattgaaca gttttctgga agtcagacgg tttcatcaat ctcaccccct ttccgttatg
    11581 acgtgaaaag tgtttatccc tctttccgca ccatataggg aatggcaagt gtgatttgtt
    11641 aagttgaaac cagaaaaaat cgaaaaaact ttttagtaaa acaaaaacag cccgtgtgaa
    11701 tgtgaataca caaacggact gctttataaa tgctatctgt atgaattttt aactcttgta
    11761 gagggtagaa tgattgtaat actgaactaa aatatttttt cagttgggaa cagcaaagta
    11821 ttattccata tacaatcatg gcgtagcccc ctttaccaga gctatacctt gtgccaccaa
    11881 tgaaaaagga cggctctggc tatatccaaa accgccccgt tatgttttca agacgcacaa
    11941 aagtatatca tctcggaacg gttatggtga tttaaaatga gaaaaacaaa agaaacacat
    12001 acttttgatt tcagaccgct agggctggca atcagagaag cccgtgaaaa agctgggctt
    12061 tctcgtaatg atttaggcga taaggtattc tatggggaac gccatatcgc agatattgaa
    12121 aatatcggtt cacacccaag ttttcaatta ttccatgatt tagttaccat gttcaatata
    12181 tcggttgatg aatatttcta tccagcagag aaagttgcaa aaagcacagc tcgccgtcag
    12241 atagaaacat cacttgattt attgagtgat aacgaattaa aaattattca aggtacgatt
    12301 gacggtatct tgaacagcag ggagaataag aagtaatcag cttcttgttt tccccgtttt
    12361 ctttttagcc atatttcttc aagacttatt tgttgatctg ctttgcagga aacaaataag
    12421 agccgacaat ctataagtta caataaccta caaattatcg gctctgcgtc ttagcgtctg
    12481 gctctttgat aatagttact tttccatgct ttatattgat taaagtttcc gttttgcact
    12541 tcggacagaa caaaggaaaa tttatcaatt ccgtatcttc tcgcacttta gttcgtgtct
    12601 tattattgca aataggacat aatacccaca tttcattttt cacagaacca cccttactaa
    12661 ttctttaaac tcgtgttttc ttcgtgattt tatagatata ttcctctggt gtagggcgtt
    12721 tatttccaaa atactttttg aaatttgcct gcacttctgg gtcggtgctg ttcataactt
    12781 catcaatcgt tgcttcaata tcagccaaaa catcagcata cgaagaacca ccagcctttg
    12841 caccagcttt caatgctttt ttaatatctc gttttttaat tcctatcaca agaaccaact
    12901 ccctaaaata cgttaatctt ctttttcaaa aattaaatct atctttcgca tacagccttt
    12961 tgcgtcaatt tctgtaggca cgaacccgca tagcgatacc cattttctgc atagctattt
    13021 ataacttcct tatgtttatc ggtgcaaagg aatacaacac ctttcgcagt ataatcaatt
    13081 tctaaatatt catatttttt catttttcct ttctttttca tattttatct gtattttttg
    13141 attgccttat ttcttaaagg aatacccaca cccaatatca atactactac caaaatagat
    13201 aaatccataa gcacacctca cttttctaaa attctttttt cttcattatt gccagactaa
    13261 taaaaattgt catatcccca taccttttca taaatctgtt cttttgtgaa cacctgtcct
    13321 ttgtggctgg aaaggaaata tagtaggtca aattctttag cagtcagata tatagacgtt
    13381 ccctcttcgt tggtaaaggt acgggttaat gggtctatat caaatccttt tttctgctga
    13441 ttaaaagaag caggggtgta agcaagttta gaggacagcc attccaccgc ttgttggaaa
    13501 acagcgtcct ctttttcagt aaattcaaca atcagtattt tcccatagta ccacctacct
    13561 taaatttatg aaaggggaaa gagtttactg ccagtgaaat accaatccct aatgaagtgt
    13621 caacaatcct attccatgcg aaccaaatag gacttatatc tttctcatgt gttactgtta
    13681 cgcataagaa tacaacgcac gttaaaaatg ttcctttttt ctgtttcaac agtacagaca
    13741 gattgatgat tggaatcaat aaaatagata aagtcaattc ccgaagtatt tctggctgaa
    13801 tgggcaaaaa aattctttcc ataagcaaaa agagcattcc cagtataccg ccgataattg
    13861 ttgaaagttc cctgttttta gcaacgctca aactgttttc catatctggc tgtatgcaca
    13921 gtatagacgc aatcactgca taaaaaggaa cacttgaagc tcttaagcta tctatcaaaa
    13981 aacaaaggaa cgcagaaagt accgtcttaa ttgtccgcaa tccaacttta acaggcttac
    14041 ttttcataca acccttttag tacagcttct tcaaggatgt gcccctccat ttcttgatag
    14101 agttcattaa gataaaatcc gttttttaaa tcaagcagtt tatcaagggc aaatacatga
    14161 agctcataaa tatgtgcttt atctggtggt gccattccac cataacagct tgtctgttct
    14221 ctggtttgct gattccccaa tatgcttgtc cagctattcg caccttgtat aaagtccttt
    14281 gctgtctggc tttcgttttc ctttaattca cttcttgtta gattagctcc taaccaatgc
    14341 acccatataa aacccgtcac tggatatgcg tctttatctt ccagtataaa ggcaaaggaa
    14401 accgttttat ctggtgcatt ttcaattttg aacggaattg aatacgttgg cacatcattt
    14461 tcattgaatt gcactccatg actaccatat tttttttcaa taattccatt agaaattcct
    14521 atacttgaaa tatacataaa ttttcctcct tgtatatcgt ttaattacag atatatatta
    14581 acaaagagga agcaaaacgt aaattaccca cttttttgta ggtattagtc tggaatcacg
    14641 tgtttctcag ttaattcatt ttccattcca tttttcctta aatattcaat ccctatatgt
    14701 tgcataattc gtaatgcttc tagtatttga tttcccatat cttccgtcaa aaaatactcg
    14761 acatgaagtg gataaccaga aaatgttttt ttatcaacaa atccataatc tataagttcc
    14821 tttaaatgtt ccaaaagcat tttttgtgta attccatcaa tatcattctc taatttagat
    14881 aatgaagtag cacctaatcg taagcgccat aaaataattg gcttccattt tccctttatc
    14941 atatcatgaa ttaattccaa aggacatgta tattcatctc gtaatttcat ttgacaaaaa
    15001 ctccgtgctt gtaataacat tagcaaattt ctgatttaaa gaagccataa atatgctttg
    15061 aactgtatct gctgggatat tgactccatt ccattttaat tccttggtcg cacaaccatt
    15121 tgatattagt gtaatctcat agccataatc tttggcagct cttatagttg tgtcaataca
    15181 catatgtgtc atcattccac aaacaaccaa tttcgttacc tcaatctcta ccagtttgtt
    15241 ttgcaaattc gtttcataaa aactatttgg ataatgttta acaataactg tttctgtatc
    15301 taatggttta atatcattat ggatttgtac accatttgta tttggattaa aaaatgttcc
    15361 atggatagac tcatggcgaa tgtaaataat tggcaagttt tgctttctaa aaattttcaa
    15421 taaatctttt attgtattca acgcaatctc tggtttatac aactggcact ttccattagg
    15481 aaaataatca ttctgcacat caataataag taatgctttt ttcatactct catatcctct
    15541 cttaattcta attaaataat ataagataat agtttctaag taaattactt actttttcgt
    15601 gtatatgata gagtttaata acaaattcct ttttcatcaa ggatttctgt cataccatgc
    15661 tctaccatgt aatctatacc aatttcttgc attatcttaa ttgctgatag catcttttct
    15721 ccctgtccag ttaaaaagta ttctactttt agaggatatc catcatatgt ttttttatca
    15781 attaagccaa agtgttttaa ttcacgcagt tgttccaata gcattttttg agtaattcct
    15841 gctatactgt gatggagttc ggaaaaagag cattttccat ttctaagttg aaaaataatt
    15901 atcgttttcc attttccctt tataatatca tgaacaagtt ctaatggaca tgtatagctt
    15961 tctctaagtt tcattttatc atctcctatg tgaaatatct tacatgttta agctcaatat
    16021 ttcaaaacat attttattac cctaatatat gcacttttaa cacattctaa atacgaacca
    16081 tgaagtataa actcatgtat ggtgatttaa aatgagaaaa acaaaagaaa cacatacttt
    16141 tgatttcaga ccgctagggc tggcaatcag agaagcccgt gaaaaagctg ggctttctcg
    16201 taatgattta ggcgataagg tattctatgg ggaatgccat atcgcagata ttgaaaatat
    16261 cggttcacac ccaagttttc aattattcca tgatttagtt accatgttca atatatcggt
    16321 tgatgaatat ttctatccag cagagaaagt tgcaaaaagc acagctcgcc gtcagataga
    16381 aacatcactt gatttattga gtgataacga attaaaaatt attcaaggta cgattgacgg
    16441 tatcttgaac agcagggaga ataagaagta atcagcttct tgttttcccc gttttctttt
    16501 tagccatatt tcttcaagac ttatttgttg atctgctttg caggaaacaa ataagagccg
    16561 acaatctata agttacaata acctacaaat tatcggctct gcgtcttagc gtctggctct
    16621 ttgataatag ttacttttcc atgctttata ttgattaaag tttccgtttt gcacttcgga
    16681 caaaatagtg ggaaattttt aagctctgtg tccgctctaa ttttcaacct cgttttatta
    16741 ccgcaaactg gacataaaat ccatttatct ttcaataatg cgttcctcct atctaagtat
    16801 acagttttgt aatttatata tctttagctt tcatcttttc catttcagtt tgctttatct
    16861 ttttatattt atccctgcca gtaccccaat ctccaaaaaa taaagtattg actacaacaa
    16921 acgggaaaat atatggtctt tcaaatggta ttctgccact tgcaaaaaca tatattgttc
    16981 ctgccaagat aagcaaggga ataatacctc cccaaagagg acttctaaat tttgtgcaga
    17041 gaagatactc tgcaattaat aaaactcctg caacaataaa gccaataatt aatgtactca
    17101 tggttatttc tccttttctt ttttatattt ttcaataatt atctttgcag tttccacgtc
    17161 aattcgagaa tcaaccgcta accctttaat aaaagtttgg aaaggttctt cttcctgctg
    17221 gtcgcacatt tcaatttttt gaattaattt gtctaaacgt aatcgaacag tagggtaaga
    17281 taccccatac attttcgcta tttcctttaa tgaaccggat ttaagtgcga agtttttaag
    17341 aaaagtagcg tcctcttgct ctaaggcaag aatccattga gggattttat caagctccaa
    17401 aaacacaact cctttcatat tatctttaac aatattcagt atatcataaa taaaattaaa
    17461 gtcaataaga atttaaacat caaaataatt attttataat cttccagttg ctcccgcttt
    17521 tttccagcac caaatcaaat tgtgatacct gcgttgcctt tgtctgctgg tcgaggtact
    17581 tcacagccac ggaaaccgtc acttgactgt cgatgcggtt gtagatggga tttaccagtt
    17641 ccgagaatac ataatctttc ccgataggct ttaacacgcc ctcatttaca taataggaca
    17701 gctctttagc ggttgcggtt gggtagagct taaagaacgt cgtcagaaat tcgttgattt
    17761 cgtccgtcgt cacagcgtcc accgttccgt cgctttcctt tgcctttggc gtgtagccgg
    17821 atttcacggg tatgctggta atggtcggat tcttcgtgag aaccatgttt ccagagccat
    17881 ccacatagac cgtgacttca taggcggagc gtaccgtctt cgtgcttcca ttctccgtga
    17941 ttaactgttc cacagtgaag atcacggaga actggttttc atccgcctgc gtgacagacc
    18001 atatctgtac cccattgacc gccgaagaca ctggaatatc cttgcggatg gtatcggtat
    18061 taagtgcctg caattccttt gtcagataac cgctgattgc ctgcgtcctt gcttcaatag
    18121 cttctttgct gttattccat gtgtaatagg acttcgcaaa gttcttcacg aaattttcaa
    18181 tcccgttggt atcggtaagt cttaactgta tggtttctgt ttcatgtacg gtgtgcatat
    18241 caatagcggt aaaattctta tataccccaa aactcacgct tgcgataagc actacccaca
    18301 atgcaatcac agatttttta tgtgtaccga ctttcacagt acgcactttc ttttctttga
    18361 cattctctgt ctgtttctta ttcttcttaa acatattttc taagtccttt cttttatttt
    18421 actcgtcctg caccgattaa gtgttgttgc cagtaactat tatttaagtc tgcatatcct
    18481 atcggatt
//
LOCUS       DAEPIN010000073        17717 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.073, whole genome shotgun sequence.
ACCESSION   DAEPIN010000073 DAEPIN010000000
VERSION     DAEPIN010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17717
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.073"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            complement(<1..1037)
                     /locus_tag="KR250_002993"
     CDS             complement(<1..1037)
                     /locus_tag="KR250_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0068890.1"
                     /translation="MQIKIGKVIQCLRKERNLTQEQLAKFIGVSTPAVSKWESGNSYP
                     DIELLPLLADFFNVSIDKLLNYKIDLSEEEVMKIYKELESGFARIEIDLSTEEPKEEF
                     RQDLESVKKLSNMYIEKYPKSYLLKLRICSLYQMYSYKFGKSELNDRVKETMNILEDI
                     VRNTDDIQIKETALIILSNAYCMLEDYEKAELYLNMIHKSIGDTSVNLAMIYLKQNRL
                     EEAEILLQNKLFSNVFNISMDCKGIINVYKNQYKELKKKLENRNFNKNAIEREMEYIK
                     NKLLGYANLSLEIKKMLSEDKGAFFSMYMDYMELSLIFLFFNMKEEAKKALYSLKEIL
                     EKYPIHESLDV"
     gene            complement(1502..2437)
                     /locus_tag="KR250_002994"
     CDS             complement(1502..2437)
                     /locus_tag="KR250_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBH0068891.1"
                     /translation="MKCTNFTFKGEEGLDIYTYKWEDENIKKPKAVIQIAHGMAETAQ
                     RYETFAKVLTKNGYIVYINDHRGHGKTAKIIENVGHLAEKEGFRCLVEDMYTLTNIIK
                     KENEDLPIYLFGHSMGSFASQRYIMDYSNNLSGLILCGSNGKQGIILNLAHLIINREI
                     KKYGRRSKSNKINNLIFGGEIIRRNEKTKFDWLSRDKEQVEKYINDPFCGVVCSCGFF
                     YDLVQGLKEIEDKENLKKVPLDIPIYIISGDKDPIGKNGKGVLRLRDRYIKLGVKDVT
                     CKLYKDGRHELLNEINREEVFEDIICWLNNKIEIL"
     gene            2725..3291
                     /locus_tag="KR250_002995"
     CDS             2725..3291
                     /locus_tag="KR250_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBH0068892.1"
                     /translation="MDYSNELKELFLMNQTYATLFTLTNKIQIEGDKYFGILTSRQYM
                     TILSILHLPEEETTLNNIARKMGTSKQNINRLVANLEKNGYVDVIPSPHDKRAINVKV
                     TDLGKKVMVTCSRTGINFMADVFHEFTKDELETLWSLLKKMYRFNGEEQDGFEEDANF
                     MEYEEIDKIKSEALEEFAKRRNRVNKKD"
     gene            complement(3482..5518)
                     /locus_tag="KR250_002996"
     CDS             complement(3482..5518)
                     /locus_tag="KR250_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HBH0068893.1"
                     /translation="MDSNRKPNNLINEKSPYLLQHAYNPINWYSWNDEAFKKAKEEDK
                     PIFLSVGYSTCHWCHVMEKESFEDEEVAEIMNRNFVAIKVDKEERPDVDSVYMTVCQA
                     MTGSGGWPMTIIMTPDKKPFFAGTYFPKYSRYNRPGVIDLLENVSEKWNTSRDILIKS
                     GDEIIEALKDDFGVKNTEGDLSKEMLSSSVRVFKAIYDEKYGGFGNAPKFPSPQNLMF
                     LMKYYSIEKDKDVLKMVEKTLDGMYRGGLFDHIGFGFSRYSTDKKWLAPHFEKMLYDN
                     AMLTIAFLDAYKITKKELYKEIAIKTIDYVVREMKDKEGGFYSAQDADSEGEEGKFYT
                     FNPLEIIEVLGEEDGIFFNNYFDITSSGNFEGKSIPNLIKNKEYERHNEKIADLSKKV
                     FEYRKERTSLHKDDKILTSWNALMIVALTKAYSTLKNDIYLEYSNKCLNFINNNLVNE
                     SGRLLARYRDGSSDYLAYLDDYAFLIWAYIELYESTFNMKYLEKALNLNESCINLFWD
                     YEKSGFYIYGKDSENLIARPKDLYDGAIPSGNSVQLYNLIRLAKITGDNRLEEMSYKQ
                     LKLYVDNVKSSPTGYSFYMLSLMFELYSTKEIICIFKEDSDLIAFKELISENFIPNAT
                     FLAKKYNEENTIIGFLNNYILKDDKTSYYVCQSNSCSQPINDLQKLKDMILGVE"
     gene            complement(5692..6369)
                     /locus_tag="KR250_002997"
     CDS             complement(5692..6369)
                     /locus_tag="KR250_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HBH0068894.1"
                     /translation="MFKHDKALLKEVKVERPNPQYAVLMQEQLGGANGELKAAMQYLS
                     QSFRIKDPQIKDLFLDIAAEELSHMEMVAQTINLLNGHDVDYNSVNTGEIETHVLTGL
                     SPVLINSSGAPWTANYVTVTGDLVADLLSNIASEQRAKVVYEYLYRQIDDKYVKETID
                     FLLNREEAHNALFRDALNKVKDTGSNRDFGVTEDSKLYFDLSTPGPNHDTKIDINPPS
                     FEKPLKK"
     gene            7086..7262
                     /locus_tag="KR250_002998"
     CDS             7086..7262
                     /locus_tag="KR250_002998"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0068895.1"
                     /translation="MEQKESKIVYSKGGSGTYSAKISLPLSQLEKMGFTREKRKAVVI
                     FKEDEIIIKKSDEE"
     gene            7810..7995
                     /locus_tag="KR250_002999"
     CDS             7810..7995
                     /locus_tag="KR250_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HBH0068896.1"
                     /translation="MILLEQRKLRVAFNKSGKGSFTPRLILPMSWIKEMNISPDERDV
                     LVTFEDGKIIIEKTENE"
     gene            complement(8036..10435)
                     /locus_tag="KR250_003000"
     CDS             complement(8036..10435)
                     /locus_tag="KR250_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HBH0068897.1"
                     /translation="MIFKKYIKSLLIVVSVTLIMNKSILFVNALDLESNNNIKSYKRK
                     NVKEIDISNKFVDESFRRDIVEKVLDNRDDPLTNEVASKDNYKIYESDLNKIESCLEL
                     DLHKNPDVSNPNKIDNLDGIENFRNLEKLDCSSNNLESVDLSKNIKLKYLDISGNKIL
                     NINLKNNNLINELHCDANKLSSLDLKNNKNLVSLFFTSNELTEIDLSNNVNLENLQFL
                     NNNLKSIDLSHNKKLKSLNCTNNKLKSLNLSNNVFLKNLSCSGNNLSKLNIKGLTSLE
                     YLGCAKNKLQDLDVSTNIKLDSLFCGKNSLTKIDISKNKELKSLECSGQYIEKSIIKN
                     GENIVVDIGIENSKLKELYPQNYNISTGFLTWKNISEFPNNVSYSLEYEYGPVNDVET
                     MDVNISIDKLVKCRIRYIDYDGSIINEKEISDNSSINEIQINPQRENYEFNGWRNNKD
                     EKIYTSEQVSKLVFDDSTDFIATYKRKSTGSSGSSGGTSSKEDFNIAPSKEKLTGADR
                     NETSVKISQKGWNKADNIVLINDSSISDALSATPFAKSKDAPILLTKNNNLNKLTEKE
                     INRLEAKNVYIVGGLKSVDEKVVSDLKKKGLNVIRISGNDRYETSIKLAKELDKNSNL
                     SKVVVVNGEKGLADAVSMGAISAKEEMPILLTNQNDDMKDIKDLIANKNISKSYVIGG
                     ESLFNNKEVNNTLPSVTKIAGSDRTETNSKVISHFYSKDTLNDLYVAKNGMNKQDDLV
                     DALSVGVLAGKTESPVVLVGNGLDDSQKELIKNKKFKNITQIGGNGNEKAFNEVENLV
                     K"
     gene            complement(10529..11341)
                     /locus_tag="KR250_003001"
     CDS             complement(10529..11341)
                     /locus_tag="KR250_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBH0068898.1"
                     /translation="MKICITVGHSILKSGACTSADGVVNEYQYNKSLAPVLADTFRKE
                     GHKADVIICPEKQFKTKAEEKTYKIPRVNSGGYDLLIELHLNASDGQGKGSEVLYYSN
                     KGLEYATRICNKLGTVFRNRRAKLDKGLYILNSSNPTAVLIESFFCDNKEDYEKAKKL
                     GHEGIAKLIVEGVLNKNINNEGVKQMYKHTIVYDGEVDKISATVVGWGYNDGKILICD
                     IKDYVPGQTQNLYVIGGAACEKIGSMTKEKFTMIKGNDRFDTLYKALDFINR"
     gene            complement(11420..12283)
                     /locus_tag="KR250_003002"
                     /pseudo
     CDS             complement(11420..12283)
                     /locus_tag="KR250_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429958.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
     gene            complement(12544..12801)
                     /locus_tag="KR250_003003"
     CDS             complement(12544..12801)
                     /locus_tag="KR250_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HBH0068899.1"
                     /translation="MDNLISFIPEQLLILVAALYVIGAGCKKYKQLDNKYIPVVLLIL
                     GIGFSIWMLGLNPVAVLQGVICWGVAIGVNQTYKQFKDGEK"
     gene            complement(12805..13095)
                     /locus_tag="KR250_003004"
     CDS             complement(12805..13095)
                     /locus_tag="KR250_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0068900.1"
                     /translation="MEEISINLLCAVAGVVISYLAFRNSSNRKIQDDTETTTKLEQQI
                     TFLCENVRDIKHDVAKFNTSFLDISERVAKVEASTKQAHLRIDEIINRIGGK"
     gene            complement(13126..13692)
                     /locus_tag="KR250_003005"
     CDS             complement(13126..13692)
                     /locus_tag="KR250_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0068901.1"
                     /translation="MTFKELVNKVRNLVLEAKNVTIEDTESKFTSENVEGALKECIDR
                     ADEAFQEADSGKTLLSTAIGSPATSEQTFQDYANYITGFKSNISNLETQLKSKYSIRY
                     GPIDGYDGNPFSANFGKSASYLIVYVYFRRSVYYYNPSGSSLGSNTGGSERAWITIDS
                     NKTGFSVHSYDTSYEPYPFTGYYIACFA"
     gene            complement(13723..13893)
                     /locus_tag="KR250_003006"
     CDS             complement(13723..13893)
                     /locus_tag="KR250_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0068902.1"
                     /translation="MYNLLKLMIEQKNYSTKEDLQHKIDVFYAVNRITEEQYLELTSL
                     LNKEETPAEPIK"
     gene            complement(13905..14252)
                     /locus_tag="KR250_003007"
     CDS             complement(13905..14252)
                     /locus_tag="KR250_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0068903.1"
                     /translation="MNRNNRIIYDQTGNIWLQTGEATGDIQEWSKITELNFLDVEFGS
                     IDYSKQYIESINPVTKEPIIKDIEVILTDEQKRLQALEKELSMLKEENKNRDSEIVNT
                     AFEVENIKLNNNL"
     gene            complement(14273..15985)
                     /locus_tag="KR250_003008"
     CDS             complement(14273..15985)
                     /locus_tag="KR250_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0068904.1"
                     /translation="MQTEWNFNYANYVQNVSLPPGRYKLECWGACGGAVDTSDWTDCA
                     KGGYSKGEIVFKKRTNLQICVGQSGYEKVSEGSSLTRSGFNGAGAAGKVTTGSFAYSK
                     YGGGATDIRLYHPSATWGNTESLLSRILVAGGGGGMKNNFASARSIGHGGGYVGVNGV
                     GRDRDFCGGGSQYQGGTSYDTEEYHGSLGKGGYGNIGIGGGGGWYGGAGSYSNECGGG
                     GSGYALNKDSYKVPGYIPTPEYYLENIVMTTGGNTTKADGYAKITLLQALPFLTVSSY
                     NSTQATFKADHTDPALLTKIEWFIDDVLKETITTNLTEEKTINYTLEDNALHTLKIVV
                     TDSNNATAEKVLSISKNIMPLPENVNLNDISTKLVEVNAGFKVGKTSIINTLALKNIE
                     ASLNNTLVELSEKIKTSFDSSDTSVQDLQNQVTQKNNTITQLETELSKRKRFITGTYT
                     FTKTDAENFNLSIYDKEGTSKTLTIPVNMGFSPSLIVLSGVTFSTTSKSYVYFDNVCN
                     SNFYNFGYNSDSTHSNPKAVGILNVSNVGYSSLVLTLYKLSMSEAVGIWAKEGATLTY
                     KIYI"
     gene            complement(16002..16994)
                     /locus_tag="KR250_003009"
     CDS             complement(16002..16994)
                     /locus_tag="KR250_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBH0068905.1"
                     /translation="MAIDKSYYTIITDVGKAKIANASVTGNKVGFVKIQLGDGGGSEY
                     TPTESQTALKNVVWEGNIGNTTTDETAPNCIILESLIPSSVGGFMIREIGYLDDENNL
                     IAISKYKECYKPSIEQGAVVDMKVKTVLIVSNVNNIELKIDPTIIFATLKDIQDLETK
                     IGTVNTKIDTTKTELTSNIETAKTELNTKIDQLIAGGSNVAYTQRVAIDDWVEDAESG
                     FKATVTHSLLTQRIVVNIIDATTKENIVPNFKIVDDNSIEIRSEVKVELNVYVINGNA
                     ETHFINATVDDNRVSEMTTYSSKKIEDRLVNIEEKVNGGLSNIATSVNELITYC"
     gene            complement(17006..17623)
                     /locus_tag="KR250_003010"
     CDS             complement(17006..17623)
                     /locus_tag="KR250_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HBH0068906.1"
                     /translation="MDKEINLINYLPQILQDKEEYIKVFNVENKEIKTLHEKLNDLSS
                     DQFLEDLTPSGIKRWEKIMSITPKSNESLEDRRFRIFSKYISKLPYSERFLRNWLDSI
                     VGEGNYELTINNATYNIHLESDARNQDWFEEVHSFVSSIKPCNMTLDYTRVLVSKDNY
                     MNFGITTLIGQEITIYPWSPPDIETYGEIDVLTGNGVGYQEITIF"
BASE COUNT         5714 a         2986 c         1907 g         7110 t
ORIGIN      
        1 cttacatcca aactttcatg tataggatat ttttctaata tttcttttag actatataag
       61 gctttttttg cttcttcttt catgttaaaa aataaaaaaa ttagagataa ttccatataa
      121 tccatataca tactaaaaaa tgcaccttta tcttcactga gcattttttt tatttctaaa
      181 gaaagatttg cataccccag caacttattt tttatatact ccatttctct ctcaattgca
      241 tttttattaa aatttctatt ctccaatttt ttctttaatt ccttgtattg atttttgtat
      301 acattaatta ttcccttaca atccatgctt atattaaata cattactaaa caatttattt
      361 tgaagcaata tttctgcttc ttcaagtcta ttttgcttta aatatatcat agctaaatta
      421 acacttgtat cccctattga cttatgtatc atatttaaat acaactctgc cttttcataa
      481 tcctccaaca tacaatatgc gtttgataat ataataagag ctgtttcttt tatttggata
      541 tcatctgtat ttctcactat atcttcaaga atattcattg tttcttttac tctgtcatta
      601 agttcactct taccaaattt atatgaatac atctgataca gcgaacatat ccttagcttc
      661 aataaatagc ttttaggata cttctctata tacatattgg aaagcttttt tacagattct
      721 aagtcttgtc taaattcttc ttttggttct tctgttgata aatcaatttc aattctagca
      781 aagcctgatt ctaattcttt ataaattttc attacttctt cttcacttaa atcaatcttg
      841 taatttaaaa gcttatcaat agacacatta aaaaagtctg ctaataaagg caataattct
      901 atatctgggt aagaatttcc actctcccac ttagatactg ctggtgttga cacacctata
      961 aactttgcta attgttcttg agttaaattt cgttcttttc taagacactg tatgacttta
     1021 cctattttaa tttgcatata tacctcccta gtacatctgt tgtaatttaa gtatataaaa
     1081 aaaatattta ctagtaaatc gagggaaagt taacaaaatg tctgtaattt aaccattggt
     1141 tatattttaa tctaaaaccc tatctcattg gcttgaaata taaaatctat taattaagtt
     1201 taagtataac ctaattaata gattcacatt aaatagcatt taaatattta gattttctct
     1261 ttattacaac ttactatatt taaactttca aggaatttta aataaaataa attccatata
     1321 caatgataat catactgagt gttaataatt tatttataat aacacccacc tattcaataa
     1381 atggcattat agcaaaaaat cacttctagg ctctctcttt tctactttgc atatacataa
     1441 tccacttttc aaatattata tataaataaa tttaaagcaa atatatcatt atattttttt
     1501 attacaatat ttctatttta ttatttaacc aacaaattat atcctcaaaa acttcttctc
     1561 tatttatttc attcaacaat tcatgccttc catctttgta aagtttgcaa gttacatctt
     1621 ttactcctaa ttttatatat ctatccctta gtcttaagac tcctttacca ttttttccta
     1681 tggggtcttt atctccagat attatgtaaa taggtatatc aagtggcact ttttttaagt
     1741 tttctttatc ctcaatttct tttaaccctt gaactaaatc ataaaaaaag ccacaactac
     1801 acactactcc acaaaatggg tcatttatat atttttcaac ttgttcttta tctctactta
     1861 accagtcaaa cttagtcttt tcatttcttc ttattatttc gccaccaaat atcaaattat
     1921 ttattttatt acttttagaa cgtcttccat actttttaat ctcacgattg attatcagat
     1981 gagcaagatt tagaatgatt ccttgctttc catttgaacc acatagtata agaccagata
     2041 aattattact gtaatccatt atatatcttt gacttgcaaa tgaacccatg ctgtgtccaa
     2101 atagataaat tggcaaatct tcattttctt tctttataat atttgttaaa gtatacatgt
     2161 cctctacaag acatctaaat ccttcttttt cagctaaatg tccaacattt tctattatct
     2221 ttgcagtttt tccatgacct ctatggtcat tgatatatac tatataacca tttttagtaa
     2281 gtaccttggc aaaagtctca tacctttgtg cagtttcagc cattccatgt gcaatttgga
     2341 taactgcctt tggttttttt atattttcat cttcccattt atatgtatat atatctaaac
     2401 cttcctctcc tttgaaagta aagtttgtac acttcataat tatccctcca gattgatttt
     2461 attcagtata ttaatttata tattatatta caagtatttt caagtcaaaa tctttctaaa
     2521 aaatttaaga attattaatt cctcatagaa attatcattg gtacatagaa gtttttacat
     2581 aaaattatac aataatttaa ttaattaata gttacataaa aaagtaatac tttaatatat
     2641 tttgacacca tattgacatt agaattaatt catgatattc tgttttcgac aaaacaacat
     2701 ctaaaaataa aggagatata tttcatggat tattcaaatg aactaaaaga gctcttttta
     2761 atgaatcaaa catatgcaac tctttttacc cttacaaaca aaatacaaat agaaggagat
     2821 aaatattttg gtatacttac ttcaagacag tatatgacta ttttatcaat tcttcattta
     2881 ccagaggaag aaacaacatt aaataatatt gcaagaaaaa tgggtacaag taaacaaaat
     2941 ataaatagac tagttgctaa tcttgaaaaa aatggatatg ttgatgtaat tccaagtcct
     3001 catgataaga gagcaattaa tgttaaagtt acagacttag gaaaaaaggt tatggtaaca
     3061 tgtagtagga ctggaataaa ctttatggca gatgtatttc acgaatttac caaagatgaa
     3121 ttagagacct tatggagttt actaaaaaaa atgtatcgtt ttaatggtga agaacaagat
     3181 ggctttgaag aagatgctaa ttttatggaa tatgaagaaa tagataaaat taaatctgaa
     3241 gcacttgaag aatttgcaaa aagaagaaat agggtaaata aaaaagatta atactatcgc
     3301 atctataata ccactttttt atagtacaat attttatata actataatgt catttttatt
     3361 ttttaacaaa acaatcatta catagattag gtgttcttag gaactctttt tctgtttttt
     3421 atacaaaaaa aggagatgat tagactttta ttttaaagca tctccttttt cgctattatg
     3481 attattctac tcctaaaatc atatctttta atttttgtaa atcattaatt ggttgactac
     3541 aactattact ttgacacacg taataactag tcttatcatc cttcaatata tagttattta
     3601 aaaaacctat aatagtgttt tcttcattgt atttctttgc taaaaaagtt gcatttggta
     3661 taaaattttc acttatcaat tctttaaatg caattaaatc agaatcttcc ttaaaaatac
     3721 atataatttc cttagtagaa taaagttcaa acatcaatga aagcatataa aagctgtagc
     3781 cagttggaga actctttaca ttatccacat acagttttaa ttgtttatat gacatttctt
     3841 caagtctatt atctccagta attttcgcaa gtcttataag attgtataat tgaacagaat
     3901 ttcctgatgg aatagctcca tcatacaaat cttttggtct tgctattaaa ttttcactat
     3961 ctttaccata tatataaaat ccactttttt cataatccca aaaaaggttg atacagcttt
     4021 cattcaaatt taaagccttt tctaaatatt tcatattaaa tgttgactca taaagctcta
     4081 tatacgccca gattaaaaaa gcataatcat ctaaatatgc taaatagtcg gaacttccat
     4141 ctctatatct agctaataat ctacctgact catttacaag attattattt ataaagttaa
     4201 gacacttatt tgaatactct aaatatatgt cattttttaa agtactatat gcctttgtta
     4261 aagcaactat catcaatgca ttccatgatg taagtatctt gtcatcttta tgcaaagatg
     4321 ttctttcttt tctatattca aatacctttt tactaaggtc agctatcttt tcattatgtc
     4381 tctcatattc tttatttttt ataagattag gaatactttt gccttcaaaa tttccactac
     4441 tagttatatc aaaataatta ttaaagaaaa ttccatcttc ttcacccaaa acttcaatta
     4501 tctcaagagg attaaatgta taaaatttac cttcttcacc ttcactatca gcatcctgtg
     4561 ctgaataaaa cccaccttct ttatccttca tctctctaac tacataatct atagttttta
     4621 tagcaatttc tttatataac tctttctttg ttattttata cgcatctaaa aaagctatag
     4681 taagcatagc attatcatat agcatttttt caaaatgagg agccaaccac tttttatcag
     4741 tagagtatct tgaaaaccca aatccaatat gatcaaacaa gcctcctcta tacattccat
     4801 ctaaagtttt ttccaccatt tttaacacat ctttgtcttt ttctatacta taatatttca
     4861 ttaaaaacat aagattctga ggacttggaa atttaggagc attaccaaaa ccaccatact
     4921 tttcatcata aatagcctta aatactctaa ctgatgaact caacatctct tttgataaat
     4981 caccctcagt attttttaca ccaaaatcat ccttaagagc ttctattatt tcatctccag
     5041 attttattaa tatatccctg cttgtattcc acttttcaga tacattctct aataaatcta
     5101 taactccagg tctattatac cttgaatatt ttgggaaata tgttcccgca aaaaaaggtt
     5161 ttttatctgg tgtcattatt atggtcattg gccatccacc actacctgtc atggcttggc
     5221 aaactgtcat atacacgcta tctacatcag gtctttcctc tttatctact tttatcgcca
     5281 caaagtttct attcattatt tcagcaactt cttcatcttc aaaagattct ttttccataa
     5341 catggcacca atgacatgtt gaatatccta cacttaaaaa tattggctta tcctcttctt
     5401 ttgctttttt aaaagcctca tcattccaac tataccagtt tataggatta tatgcatgct
     5461 gtaaaagata tggagatttt tcatttatta gattattagg ttttctatta ctgtccaaaa
     5521 taccacctca tttgttattt tattatatgt tttgcaataa acaaatagat atacacttat
     5581 aataagtgta tacccatttt taaccataca tatttaattt ttcatataat taattctcct
     5641 agtaaataat ttttataact actaaaacaa aacaacataa aaaatatcaa attatttttt
     5701 taaaggtttt tcaaaagaag gaggattaat atctatctta gtatcgtgat ttggccctgg
     5761 agtagacaaa tcgaaatata atttagaatc ttctgtaact ccaaaatctc tatttgaacc
     5821 tgtatcttta actttattta aagcatctct aaatagagca ttatgtgctt cttctctatt
     5881 aagtaagaaa tctattgttt cttttacata tttatcatct atttgtctat acaagtactc
     5941 ataaactacc ttagctcttt gttcagaagc tatatttgaa agtaagtcag ctactaaatc
     6001 ccctgtaact gtaacgtaat tagcagtcca aggagctcct gatgagttta ttaagactgg
     6061 tgatagacca gttaatacat gtgtttctat ttcgcctgta ttaactgaat tataatcaac
     6121 atcatgtcca tttaataaat ttatagtttg tgctaccatt tccatatggc taagctcttc
     6181 tgctgctata tcaagaaata aatcttttat ttgagggtct tttattctaa aactttggga
     6241 caagtattgc atagctgctt ttaactctcc atttgcacca cccaactgtt cttgcattaa
     6301 aacagcatat tgaggatttg gtctttctac ttttacttct tttaataatg ctttatcgtg
     6361 tttaaacata tgaatacctc cgtaaattat tattcaaaat tattatttac ttaagttgta
     6421 atattattct aggttatttg tttattgttg ttatttttta caagtgttgg tatttcaatg
     6481 ttttattaag gtcagttttg ttgtttatgt tttattatgt ttaatttaaa tttattctat
     6541 tatttatttt cggtggcaaa agggtggcaa gtttggtttt gccaccctta tttttatttg
     6601 taaaaaaata tagtttgtca atataacttt attgaaaaat atggaaataa atatcgcttt
     6661 tgccggttcg gcatattata atataaatat agaaaggggg tgaaaaggaa aatgagtaga
     6721 atagaaagaa gaaaaaaaga tagagaaagc aaactcgcca aagttaaaac tgctctctct
     6781 atcttaccca ttttaataaa tttaattgat aaactaatta aattattaaa agatttatag
     6841 ttttgtaagc taggaagaat ttaatcttct tcctagtgac ttctttctaa atattataac
     6901 acattttcta taaaatatga gtgataaagt ttataaattc tgtcttatta tagttgctat
     6961 aactatagta atagacataa tacttttatt tttacatttt aatatcaaca atataatagg
     7021 attaatagta agcattttat tgttaatatt tgttacatta caatataaaa aaggaggaaa
     7081 atagtttgga gcaaaaagaa tctaaaatag tttacagtaa aggtggttca ggtacttatt
     7141 cggctaaaat ttctttacct ttaagccaac ttgaaaaaat gggatttaca agggaaaaac
     7201 gtaaggctgt agttattttt aaggaagatg agattataat aaaaaaatct gatgaggaat
     7261 aactctaaaa taagtgggtg tataatgtaa acatccactt atttttatta tattttgtat
     7321 tttatattaa tatattttat agctttattg aaaaatatgg aatatttatt gactttcacg
     7381 tcacgtgatg atataatata aatatagaaa ggaggtaaga aaataaaaaa cttagaaggg
     7441 aggacaaaga aagtggggag attagaaaga agaaaaaata aaagagaaaa caaatttaat
     7501 aaaattaaaa atgctttctc tttcacatta gttttaatta atctagtact cgcaatacta
     7561 agattgatta aagaattata agtttcaatc ctaggaggaa aataccttcc ttctaggtag
     7621 cttctttcta atattataac acattttcta atttaatatg aataataaat tttatagaat
     7681 ttgtatgatt attcttggta tcactatagt tttaaatatt attaatataa taataagttt
     7741 tacttttaaa gctcttattg ctttggtatt tactttatta ttgctggcat ttattaatag
     7801 tagaaaggaa tgatattatt ggaacaaaga aaacttagag ttgcattcaa taaaagtggc
     7861 aaaggctctt ttacacccag attgatactt ccaatgtcat ggattaaaga gatgaatatc
     7921 tctcctgatg aaagggatgt tcttgttact tttgaggatg gaaaaataat tattgaaaaa
     7981 actgaaaatg aataaaaaag gaggtagtaa ctatttccag ttactacctt tcaatctatt
     8041 taactaagtt ttctacttca ttaaatgctt tttcatttcc atttccacca atttgagtta
     8101 tatttttaaa ctttttattc ttgattaact ctttttggct atcatctaat ccatttccaa
     8161 ctaatactac tggagattca gttttacctg caagcactcc tactgataag gcatctacta
     8221 aatcgtcttg tttattcatt ccattcttag ctacatataa gtcattaagt gtatctttgc
     8281 tatagaaatg acttattact tttgagttag tttctgttct atcagaacct gctattttag
     8341 ttacagatgg taatgtgtta ttcacttctt tattattaaa taaagattct ccacctatta
     8401 catatgattt tgatatgttt ttattagcta ttaagtcttt tatatctttc atatcatcat
     8461 tttgattagt aagaagaatt ggcatttcct cttttgctga tatagctccc atacttactg
     8521 catcagctag tcccttttct ccatttacca ctactacttt tgacaggtta gaatttttgt
     8581 ctaattcttt tgcaagtttt attgatgttt catatctatc atttccagat attctaatta
     8641 catttaatcc ttttttcttt aaatcagaca ctactttttc atcaacagac tttaatccac
     8701 ccactatgta cacatttttt gcttctaatc tatttatttc tttttctgtt aatttattta
     8761 agttattatt tttagtaagt aatattggtg catctttaga cttagcaaat ggtgttgctg
     8821 ataaagcatc cgatatgcta gagtcattta ttaacactat attatcagct ttattccatc
     8881 ctttttgact tattttcact gatgtttcat ttctgtctgc acctgttaat ttttccttag
     8941 atggtgctat attaaaatct tctttacttg aagttccacc agaagaacca cttgatcctg
     9001 ttgattttct tttataagtt gctataaaat cagtactatc atcaaaaact aatttactta
     9061 cctgctctga tgtatatatt ttttcatctt tattgtttct ccaaccatta aactcatagt
     9121 tttctctttg aggattaatt tgaatttcat ttatactaga attatctgat atttcttttt
     9181 cattgattat acttccatca taatctatat atctgattct acatttaact aatttatcta
     9241 ttgatatatt tacatccata gtctctacat catttactgg accatattca tattccaaac
     9301 tataacttac gttatttgga aattcagaaa tatttttcca tgttagaaaa cctgttgaga
     9361 tattataatt ttgtggatat aattccttta atttcgaatt ttcaattcct atatctacaa
     9421 ctatattttc accatttttt ataatacttt tttctatata ttgaccacta cattccaatg
     9481 attttaattc tttattttta cttatatcta tttttgttaa actatttttc ccacaaaata
     9541 aagaatctaa ttttatattt gtagatacat ctaaatcttg taatttattc ttagcacatc
     9601 ccaaatactc aagacttgta agtcctttta tatttaattt acttaggtta ttacctgaac
     9661 aacttaaatt tttcaaaaaa acattattac ttaaattaag agatttaagt ttattgttag
     9721 tacaatttag agatttcaac tttttattat gacttaaatc tatacttttt aaattattat
     9781 taagaaattg caaattttct aagttcacat tattacttaa atctatctct gttaattcat
     9841 tactagtaaa aaataaactt accaaatttt tattattttt aaggtctaaa cttgataatt
     9901 tatttgcatc acaatgtaat tcattaataa gattattgtt ttttaaatta atgtttaata
     9961 ttttatttcc actaatatct agatatttta attttatatt tttacttaaa tctacacttt
    10021 ctagattatt tgaactacaa tcaagctttt ctagatttct aaaattttct atcccatcta
    10081 aattatctat tttgtttggg ttggaaacat ctggattttt atgtaaatct aactctaagc
    10141 aactctcaat tttatttaaa tcactttcat atattttata gttatctttt gatgcaactt
    10201 catttgttaa tggatcatcc ctattatcta ataccttttc aacaatatct cttctaaagg
    10261 attcatcaac aaatttattg cttatatcaa tttcctttac attctttctt ttataagatt
    10321 ttatgttatt gttactctct aaatctaatg catttacaaa taatattgat ttgttcatta
    10381 tcaatgtaac actaacaact atcagtaatg attttatata cttcttaaat atcataatct
    10441 acccccatac ctattaataa taaataatat ttatctaaat attaccatac ttatgatttt
    10501 caagaaatac taaaatattt tatataatct atctattaat aaaatctaat gccttataaa
    10561 gtgtatcaaa tctatcatta ccctttatca tagtaaattt ttctttagtc atagaaccaa
    10621 tcttctcaca tgctgcacca ccaatgacat aaagattttg cgtctgacct ggcacgtaat
    10681 cttttatatc acatatcagt attttcccat cattataacc ccaaccaact acagttgcag
    10741 agattttgtc aacttctcca tcataaacaa ttgtatgttt gtacatctgt ttaactccct
    10801 cattatttat atttttattt aatacacctt ctacaattaa cttagcaata ccttcatgac
    10861 ctagtttctt agctttctca taatcttctt tattatcaca gaagaaactt tcaattaata
    10921 ctgctgtagg atttgaacta tttaagatat ataatccttt atctaattta gctcttctat
    10981 ttctaaatac tgtacctagc ttattacata ttctagttgc atattctaaa cctttattac
    11041 tataatatag aacttctgaa ccttttcctt gaccatcact tgcatttaga tgtagttcta
    11101 taagtaaatc atatcctcca ctattaactc taggtatttt ataagtcttt tcttctgctt
    11161 tagttttaaa ctgcttttca gggcatatta ttacatctgc cttatgacct tcttttctaa
    11221 atgtatctgc taatactggt gcaagagatt tattgtattg gtattcgtta actactccat
    11281 cagcagaagt gcatgcacca ctttttaaaa tactgtgtcc tactgtaata catattttca
    11341 ttatttattt tcctccttct tttcaataaa aaaacactta catatagtaa gtgtttataa
    11401 acttcttaaa gtaactattt tatttagcca atatctctat ccaattatta ggaaaaccaa
    11461 taagctttaa gtctatagaa ttattataat cattaattaa cttttgaagt tttataaaaa
    11521 aactattcca aattaattta tccatagtca aatgcttcat agcaagtata taagaaaata
    11581 tctttttatt atccaagtta tactttttat attcgttttt tattgttata atacggaaat
    11641 tattattata tattctacca taatgagcgc attgatttct gacctgtgtt aatgattgta
    11701 accaagattt aactaataaa gtattcactc tacacaaatt attttttatg tatctagtat
    11761 cttctggcaa catgtttgaa tataattttt acaacatacc aaaagtcatt atttccgttg
    11821 caacccaaat gggaagtttt cctttatatt tttctttatg gtgtataata aaaagcttat
    11881 ctgaattatt atttttttct ctctctaatg ctgtcaaaaa attaatatag aatttttcat
    11941 ctttgaaact ctccctttat agatatccac aagcaccatg tttaattgct aatgtatatg
    12001 caatgtatgt cctaaatgct atttctatgc tccctaacaa atctgttaat aatatcctaa
    12061 attccttatt aaacctatat atatcataaa cttcttcaaa cgtagttcct tctttatatg
    12121 agccatcatc attcttaaaa cttagaagat atgctgtaaa cctataataa ttgacattac
    12181 ttaatacaaa tttagcatct tctttatcat ttattattaa tcctctactt tttaaaatat
    12241 ctatttgttc atcaaatgtt ttttcttctt ttacttcaac cattgttttc accttttcat
    12301 agatatactt ttatataata aaacccacca attaatatta gtgggtaaat ttatctgtcc
    12361 cgactatttg agctataaaa gaagcttggc gggttccttc aaaaatcaat gtcccgtata
    12421 tttgagcata taaatgcttt acgagttctg tactattatt atatacttat tagctataaa
    12481 agtatactct atttaatgaa attttttaaa tttttgtgag taataatatt tttgttaatt
    12541 tcattacttt tcaccatcct taaactgttt gtaagtttga tttacaccta ttgcaactcc
    12601 ccaacaaatt acaccttgta agactgcaac aggatttaat cctagcatcc atattgagaa
    12661 acctattcca agtatcaata acactactgg aatgtattta ttatctaatt gtttatattt
    12721 tttgcaacct gctcctataa cataaagagc agctactaaa attagtaact gctctggtat
    12781 aaaacttatt aaattatcca tcttttattt tcctccaatt ctatttataa tttcatcaat
    12841 tctaagatgt gcttgttttg tacttgcttc tacttttgca actcgttcac tgatatctaa
    12901 gaaacttgta ttaaactttg ctacatcatg ctttatatct cttacattct cacacagaaa
    12961 agttatttgt tgttctaatt ttgtagttgt ttctgtatca tcttgtatct ttctgtttga
    13021 gctatttcta aatgctaagt aggatattac aactcctgca actgcacata atagatttat
    13081 gctaatttct tccatacatc ctcctagttt taataatata atttattatg cgaaacaagc
    13141 aatataataa cctgtaaaag gatatggctc ataactagta tcatatgaat gaactgaaaa
    13201 accagtttta ttgctgtcta tagtaatcca tgcacgttca gaacctcctg tattacttcc
    13261 taaagaacta ccactgggat tataataata tacacttctt ctaaagtaaa catagacaat
    13321 aaggtaactt gcactcttgc caaaattagc agaaaaagga ttaccatcat atccatcaat
    13381 aggaccatat ctaatagaat atttgctttt caattgagtt tctaaattgc ttatattgct
    13441 cttaaatcct gtaatatagt tcgcataatc ttgaaatgtt tgttctgatg tagcaggaga
    13501 gccgatagca gttgataaaa gtgtttttcc actatcggct tcttgaaaag cctcatctgc
    13561 tctatctata cattctttca atgctccttc tacattttca cttgtaaatt tactttctgt
    13621 atcttctata gttacattct ttgcttctaa tacaagattt ctaactttat taactaactc
    13681 tttaaaagtc atttagtcac cttctttcaa taaaaaaaga acctatttta ttggttctgc
    13741 tggtgtttct tctttattta ataaacttgt aagttctaaa tattgttctt ctgtaattct
    13801 atttactgca tagaatacat caattttgtg ttgcaaatcc tctttagtgc tatagttctt
    13861 ttgttctatc ataagtttta ataagttata catgttaatt cctcctataa attgttattt
    13921 aattttatat tttctacctc aaaggctgtg tttactatct cactatctct atttttattt
    13981 tcttctttta acatgcttaa ttctttttct aatgcttgta atctcttttg ttcatctgtt
    14041 aaaatgactt ctatgtcttt gatgattggt tcttttgtaa ctggatttat agactctata
    14101 tattgtttac tatagtctat actaccaaat tcaacatcca aaaaatttaa ttcagttatt
    14161 tttgaccact cttgtatatc tcctgttgct tcaccagttt gaagccatat attgcctgtt
    14221 tggtcgtaaa ttattctatt atttctgttc atattatcac ctcatttttt tattaaatat
    14281 atattttgta agttaatgta gctccctctt ttgcccatat tccaactgct tctgacattg
    14341 ataatttata taatgtaagc actaatgaag aatacccaac attagataca ttaagaatac
    14401 caacagcttt cgggttacta tgagtagaat cagaattata gccaaaatta taaaaatttg
    14461 aattacaaac attatcaaaa taaacataag atttacttgt tgtactaaaa gttactccac
    14521 taagtacaat taaactagga gagaagccca tgttaactgg tatagtaaga gtttttgaag
    14581 ttccttcttt gtcatatata cttaagttaa aattctctgc atccgttttt gtgaatgtat
    14641 aagttcctgt tataaatctt tttcttttac ttaactctgt ttctaattga gttatagtat
    14701 tgtttttttg tgttacttga ttttgtaaat cttgcacact agtgtctgaa ctatcaaaac
    14761 ttgtttttat tttctctgat aactccacaa gtgtattatt taaacttgct tctatatttt
    14821 taagtgctaa agtgtttata atacttgttt tcccaacttt aaatcctgca ttaacctcaa
    14881 ctaattttgt tgatatatca tttaaattta cattttcggg cagtggcatt atattcttac
    14941 ttatacttaa cactttttct gctgtagcat tattactgtc tgtaacaact attttaagtg
    15001 tgtgtaatgc attatcttct aatgtataat taattgtttt ctcttctgtt aaatttgttg
    15061 ttatagtttc ttttagtaca tcatctataa accattctat ctttgtaagt aatgcagggt
    15121 ctgtgtggtc ggctttaaat gttgcctgtg tagaattata agaagatact gttaaaaatg
    15181 gcaatgcttg tagtaatgtt attttagcat aaccatctgc tttagtagta ttacctccag
    15241 tagtcatgac tatattttca agataatatt caggtgttgg tatatatccg ggtaccttat
    15301 aactatcttt atttagtgcg taaccacttc caccacctcc acattcatta gaataagaac
    15361 cagcaccacc gtaccaaccc cctccaccac ctattcctat gttaccataa cctccttttc
    15421 ctaatgaacc atggtattct tctgtgtcgt aacttgttcc accttggtat tgagaaccac
    15481 cgccacaaaa atctctgtca cgaccaactc cattaacacc tacataacca ccaccatgac
    15541 caatagaacg agcagaagca aaattatttt tcatacctcc tccaccgcct gcaacaagta
    15601 tgcgtgaaag caaactttcg gtgttacccc aagttgcact aggatgataa agtcttatat
    15661 cagttgctcc accaccgtat ttagaataag caaagctacc agtagtaact ttgccagcag
    15721 cgcctgcacc gttaaaacca cttctagtaa ggcttgaacc ttcagaaact ttctcataac
    15781 cggattgacc gacacaaatt tgtagattag ttcttttttt aaatacaatc tcaccttttg
    15841 aataaccacc ttttgcacaa tcagtccaat cgcttgtatc gacagcacca ccacaagcac
    15901 cccaacattc taatttatat cgcccaggtg gcaatgaaac attttgtaca taattagcat
    15961 aattaaaatt ccattcagtc tgcattttct cactctcctc tctaacaata agttatcaac
    16021 tcatttacac ttgttgcaat attagataaa ccaccattta ccttttcttc tatattaacc
    16081 aatctgtcct ctattttctt agatgaataa gtagtcattt cagatactct gttatcatct
    16141 acagttgcat ttataaaatg agtttctgca tttccattta tcacatagac atttaactca
    16201 acttttactt cacttctaat ctcaattgaa ttatcatcaa ctattttaaa atttggaact
    16261 atattttctt ttgtagtagc atctataata tttacaacta ttctctgtgt taataaacta
    16321 tgtgttacag ttgctttgaa tccactttct gcatcctcaa cccaatcatc aattgcgacc
    16381 ctttgagtgt atgcaacatt tgagccacct gcgattaatt ggtcgatttt agtatttaac
    16441 tctgttttag cagtttctat gttgcttgtt aattctgttt tagttgtatc aattttagta
    16501 ttaacagtac ctattttagt ttctaagtct tgtatatctt tgagtgttgc aaagattatt
    16561 gttgggtcaa ttttaagttc tatattattt acattagata caataagcac agttttaacc
    16621 ttcatgtcta ccactgcacc ttgttctata gaaggtttat aacactcttt gtatttagaa
    16681 atggcaatta aattattttc atcatctaaa tatcctattt ctcttatcat aaacccgcct
    16741 acacttgatg gtattaaact ctctaatatt atacaatttg gtgcagtttc atctgtagtt
    16801 gtatttccga tattgccttc ccataccaca tttttgagag ctgtctgact ctcagttgga
    16861 gtatattcac tccctcctcc atcaccaagt tgaattttta caaatcccac tttattacct
    16921 gtgacacttg catttgctat ctttgctttc cctacatctg taattatagt gtaataactt
    16981 ttatctatag ccaatatatc acctcctaaa atattgttat ctcttggtat ccaactccat
    17041 tgccagttaa tacatcaatt tctccataag tttctatatc tggtggactc caagggtata
    17101 tagttatttc ttgacctatt agggttgtta taccaaaatt catataattg tctttactta
    17161 caagcactct agtgtaatct aaagtcatgt tgcaaggttt aatactactt acaaaagaat
    17221 gaacctcctc aaaccaatct tgatttctag catcactttc aagatgtata ttataagtag
    17281 cattattaat agttaattca taattgcctt ctccaactat actatctagc cagttcctta
    17341 aaaatctctc tgagtaaggt agtttactta tatatttact aaaaatccta aaccttctat
    17401 cttctaaact ctcattactt ttaggagtta tagacattat cttttcccat ctttttatac
    17461 cacttggagt taaatcctct aaaaactggt cacttgatag gtcatttaat ttttcatgta
    17521 gtgtttttat ttctttgttt tctacattaa atacttttat atattcttct ttatcttgta
    17581 gaatttgtgg taagtaattt attagattaa tctctttatc caactacctc acctctcact
    17641 actatactgt tactatctat tgttagatta gatttaacat catttatcat tgtgtttgta
    17701 atatctaaca ctccatc
//
LOCUS       DAEPIN010000074        17557 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.074, whole genome shotgun sequence.
ACCESSION   DAEPIN010000074 DAEPIN010000000
VERSION     DAEPIN010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17557
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.074"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            131..1990
                     /locus_tag="KR250_003011"
     CDS             131..1990
                     /locus_tag="KR250_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBH0068907.1"
                     /translation="MKRFLRRIILVLFILLIFISFISIKLIHNVGDYGKLINYVGIVR
                     GASQRLTKLEMNHQPNDELIEYIDEILQELITGHGDYGLVITDCNEYNEDLLLLEKKW
                     EDLNIEIKKVRMKEQNNQLLSISEEFFSLANDTVFKIENFSKEKSNYLMTLIIIISII
                     GILACIILILQYSKKMVRLEKLNVDLKNIAYKDELTGVNTIEKFKLDANQNICIHKDK
                     KFAVFYIDFENFKYINDIFGYDYGDMILKRYANLMMNDIGKYEIFAREIADRFVALRC
                     YVDKEDLVVRQRDVDNELINITNEIKNKHSITVVSGICCIEDVNEKLSIDGLINRANF
                     AQKTVKNKPGTNYAFYNDSIRKKMIEENTIKSRIHEAIEKREFIVYLQPKVNLHSQKI
                     NCAEALVRWLTPDKGIISPAIFIPVLEKNFFIALVDKYVFEEVCKWIRKRLDENKPFV
                     QISVNVSRIQFYNTKFVETYSNIQNKYRIPKNTIEIEFTESVAFENQKHLLEIIHDLH
                     ENGFTCSLDDFGKGYSSLSVLKDLPFDVLKLDSMFFKASLDKDKEKIVIKNIVHMLKE
                     LNITTVAEGIECKEQVEFLRDIGCDLVQGFVFYKPMPILEFEKILDKEFVYNS"
     gene            complement(2282..2881)
                     /locus_tag="KR250_003012"
     CDS             complement(2282..2881)
                     /locus_tag="KR250_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBH0068908.1"
                     /translation="MAIELKKGQKINLTKKENSDLGEILVNLNWNQKVQKKGFFGSLR
                     SSNIDLDLGCLFEMKNGVKGAVQALGNAFGSLDNPPFAQLDGDDRTGSNTQGENLRIN
                     GNKIKDIKRILIYAFIYEGVANWSEADGIVTIKQKQDSDLVVKLDEHKNGYNMCSIAL
                     IENVNDETFSVEKVVKYFKGHREMDTEFHWGLKWVAGRK"
     gene            complement(2984..3781)
                     /locus_tag="KR250_003013"
     CDS             complement(2984..3781)
                     /locus_tag="KR250_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBH0068909.1"
                     /translation="MKFKKLLCLLLCLVLTLAVVGCSKAKDDKKIVVGATLVPGGELL
                     EELKPLIKEKGYTLEVKNFDDYILPNEALNNGEIDANLFQHEPYLKEAVKAKGYKIMA
                     GKKLYVCPAILYSYKIKSVDEFKKGDTIAISNNPSSCSKNLRYLESIGLLTLPKGDGL
                     VSPKDIIENPKGIQFKELDIAQIPSSLPDVTAAFIDTTYAVPAGLDAKKNGIYTAPIN
                     DEYANLLAFRTEDKDSEKIKVLQDVLTSDKARSLIEEKYKGIVIPTF"
     regulatory      complement(3894..3999)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(4091..5077)
                     /locus_tag="KR250_003014"
     CDS             complement(4091..5077)
                     /locus_tag="KR250_003014"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HBH0068910.1"
                     /translation="MLLIYNEKTNPYFNLAMEEYLLKNFDEDLFILWRNEPSIIVGKN
                     QNTLSEINLEYIKEYSIPVVRRQSGGGAVFHDLGNINFTFIACNNNNFSDFRRFTQPI
                     IDLLKTLDVNAEFSGRNDLLIDGKKFSGNAQYNYKNKVMHHGTLLFSSEINDLSKALK
                     VKPIKFEGKGIKSVKSRVTNISEHLHSKMDILEFKDAIMDYLSKTNTDNTNYCLSEHD
                     IKQIEKLTKSKYETWDWNFGNSPKYTLSNELKYSGGNVEFNLNVDKGIITSIKFFGDF
                     FGKCDVAFVENKLIGIRHEENALRNTLDSIEINDYFLGADTDILVSGILGVK"
     gene            5561..7291
                     /locus_tag="KR250_003015"
     CDS             5561..7291
                     /locus_tag="KR250_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HBH0068911.1"
                     /translation="MRNKKINIIFLTTIMFIMSTVMVFAEEDIDTIALANAEKFGILT
                     LIPPLVAIILAFITKNVIISLLIGILSGSFIIKASGINVFATFIQAFLDLVDRALVSL
                     ADPWNAGIILQVLAIGGVINLVAKMGGAKAIAEALAKRAKSAKGTQLITWFLGLLVFF
                     DDYANSLIVGPMMRPVADKMKISREKLAFIIDATAAPVAGLAIISTWIGLEVGLIHDA
                     FESISIDVDAFGIFLNTIPFRFYNILILAFIVISALLLKEFGPMRKAEIKSRSRKISI
                     DLDEGVEELDDLAPKNGVKLSVWNAIIPIGTLIIVALASFYYSGYTSIMGGDDKALIQ
                     LFTNSPYSFEAIKEAFSASDASRALFQSALVASLVAIIMAVVKKIFTISEAIDVWIDG
                     MKSLVITGVILILAWSLSSVIKELGTAKFLIHLLSGSLPPFLLPSLIFGLGAIISFAT
                     GTAYGTMGILMPLAIPLAYSLNPDMSYVIVSTSAVLTGAIFGDHCSPISDTTILSSMG
                     AGCNHIDHVNTQMPYAIFTAVITIVFGYIPAGLGLPIYIVLPVAIAAIFVGIQIIGKK
                     VDEAEIELVE"
     gene            7401..7481
                     /locus_tag="KR250_003016"
     tRNA            7401..7481
                     /locus_tag="KR250_003016"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7435..7437,aa:Leu,seq:tag)
     gene            7816..8331
                     /locus_tag="KR250_003017"
     CDS             7816..8331
                     /locus_tag="KR250_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HXXEE domain-containing protein"
                     /protein_id="HBH0068912.1"
                     /translation="MEKYIWLFPLLFIFHDMEEIIGFGIWLKKNKSMLDKKYPFISKV
                     YENYSTEGMAFAVFEEFILCIIFCILTVITENQYVYLLWLGSFIAYTLHLVIHIGQSI
                     IIRKYIPSLITSIICLPISIWCISKSIYIVDCEMSTTILYSIIGIIIVALNLKFAQSL
                     IGKFTKWMSNI"
     regulatory      8823..8927
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            9137..11611
                     /gene="gcvPA"
                     /locus_tag="KR250_003018"
     CDS             9137..11611
                     /gene="gcvPA"
                     /locus_tag="KR250_003018"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HBH0068913.1"
                     /translation="MNELKRVSLYNIHKELGAKLVEFAGWEMPLEYEGINKEHEKVRK
                     SAGIFDVSHMGEVQIKGAESEKFIQNLVTNDISALKINDIIYTPMCYENGGVVDDLLI
                     YKFGEEDYLLVINAGNIDKDVAWIIKQSEGYNVDIKNISSEVSQLAIQGPKAEEILQK
                     ITDIDLNSIKFYKSIPSTKVCGCPCLVSRTGYTGEDGFEIYCKNKYVEIIWNEVLKVG
                     GEDICPAGLGCRDTLRFEAALPLYGHEINEHISPIEGGLSIFVKTNKESFIGKSILSK
                     EKESGAKRKLVGFEMQGKGMPRNGYDIRIGDKTVGFVTTGCASPTTGKILGMGIIDSE
                     YAKVGNEIGIAIRKKVVPAVIVKKPFYKKQYKKDNIILNKENKFSYIPATSEDKSKML
                     KVVGLNSVDELFSDIPEEVKLKRDLNLEIGKSELEVSKIVKRLSEENLSLEDLTCFLG
                     AGAYDHYIPSIIKHITSRSEFYTAYTPYQAEISQGTLQVVFEFQSMIAEITGMEIANA
                     SMYDGATAAIEACIMAMNQTRKSKIVVSKTIHPETLSILRTYLQYKDCEIVEIDFCNE
                     YGTTDIEKLKASVDKDTACVLIQTPNFFGIIEEMEEIEKITHENKAMLIMSVDPISLG
                     VLKTPGEIGADIVVGEAQSLGNPLNFGGPYVGFLASKSKYTRKMPGRIVGQSLDVEGK
                     IAYVLTLQTREQHVRREKATSNICSNQALNALVASIYIATMGKEGFKEVGMQSMKKAH
                     YTYNKLVQTGKYKPIFKGKFFKEFAVQGNLNIETINDKLLEENILGGYNLEYNYPELK
                     NSTLLCVTEKRSKEEIDKLVGIMEGL"
     gene            11611..13068
                     /gene="gcvPB"
                     /locus_tag="KR250_003019"
     CDS             11611..13068
                     /gene="gcvPB"
                     /locus_tag="KR250_003019"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HBH0068914.1"
                     /translation="MKEYNSLLIDISKKGRKAYSLPKLDIDDIKIENMIDQDMARQSE
                     LNLPEVGELELVRHYTLLSNKNFGVDTGFYPLGSCTMKYNPKINEDMAALPNFTGMHP
                     YQSSDTAQGSLSLMYDLSRRLAEITGMDEVTLQPSAGSHGEFTGLMIIKAYHENRGDH
                     KRTKVIIPDSAHGTNPASAAMANFDVIQIASDKNGAVDINALKEVLNDEVAALMLTNP
                     STLGLFEKNIKEIATLVHEAGGLLYYDGANMNAIMGITRPGDMGFDVVHLNLHKTFST
                     PHGGGGPGAGPVGVKKELVPFLPIPVIERKEDKYVLNYDREKSIGKIKNFYGNFGVLV
                     RAYTYILTMGRDGLKEASEMAVLNANYIKESIKDDYILPIDTLCKHEFVLGGLPRGEA
                     NIKTIDIAKRLLDYGYHPPTMYFPLIINEALMIEPTESESIETLDSFIEAMKSVAKEA
                     KEEPELLKTAPHNTLVKRVDDARAVKKPILTWSQR"
     gene            13491..15848
                     /locus_tag="KR250_003020"
     CDS             13491..15848
                     /locus_tag="KR250_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HBH0068915.1"
                     /translation="MDKVNLERYSCDINVGLNKNQLQSRIDDNLINAFDNGKTKTYKQ
                     IFKDNIFTLFNLINIVLVCLLVSVGSFKNTLFIFIAVINTIIGVIQEIRAKRLLDNLS
                     VIVSNKVSVIREKEKKEIDVQELVLDDIMILKTGNQICADSKVLNGKLEVNESLVTGE
                     SDIIIKNKGDFLYSGSFVVSGEAFVRVENIGKDNYANKIANDAKISKKHNSQLRNSLN
                     AILKIVGIIIIPLGIILFAKQHFGNGDSIATSVVGTVAAVNGMIPEGLTLLTSIALAV
                     GTIKLASHKTLVQELYCVETLARVDTLCLDKTGTITEGKMQVDDIVMLEEVDINEIMG
                     NICNSLKDDNATLNAIRDKYNVLDTYKAKNLIPFSSERKYSGVTFENKGTYLLGAFEF
                     IFKEKNKKLRIEVERYSKKGNRVIVLAHSDSYMEDNNIPEDIKPLAFILILDKIRDEA
                     KETLEFFYNEGVDIKIISGDNPITVSEVARKAGLKTASNFIDATTLKNDSDIYKAVDK
                     YSVFGRVSPQQKKQMILALKKQNHTVAMTGDGVNDVLALKEADCSIAMASGSDVTKNV
                     SNLVLLDSNFASMPKVVMEGRKVINNIQRASSLFLVKTIFSFFLSLLTLFFFSRQYPF
                     VPIQLSLIGAVTVGIPSFFLALEANKSRISGNFLLNILKNALPGALCVTVNVIIVYNI
                     VEYLGYSSSVFSTMCAILTGVCGLLILRQQCLPFNKERLFLIVSMTIAFVIGILFLGG
                     LFSFVALSRELILITMALAILMPIMIKVMLFVLDEILETIVPDLN"
     gene            complement(15934..16251)
                     /locus_tag="KR250_003021"
     CDS             complement(15934..16251)
                     /locus_tag="KR250_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HBH0068916.1"
                     /translation="MSEFKNVTAVKKANVYFDGKVSSRVIILPNGERKTLGLMLPGEY
                     TFSTREEEIMEMLAGSMDVKLPGSNEFVTYKEGQKFNVPSDSSFDLKVNEVVDYCCSY
                     IAD"
     gene            17161..17268
                     /locus_tag="KR250_003022"
     CDS             17161..17268
                     /locus_tag="KR250_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013483530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBH0068917.1"
                     /translation="MGRHLWNPSYFVATVSENTEEQIRKYINNQKKQVK"
     gene            17270..>17557
                     /locus_tag="KR250_003023"
     CDS             17270..>17557
                     /locus_tag="KR250_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH0068918.1"
                     /translation="MVVVEKAYKFRMYPNKKQQELINKTFGCCRFVYNKYLAKRIDVY
                     KNNKETFTYKQCSSDLTNLKKELKWLKEPDKFSLQNALKDLDNAYKKFFKEK"
BASE COUNT         6669 a         2037 c         2890 g         5961 t
ORIGIN      
        1 ctatgtaaaa caaggtttat atattctaat tgtatagaaa atttattgta aacgcatgta
       61 tattattaga aaaaatagtt tgaaggtatt caataaacat aaaaaacaat ataggggata
      121 aaggtgtttt atgaagagat ttttgaggag aattatctta gttttattta ttttgttgat
      181 ttttataagt tttatttcta ttaaattaat tcataatgtt ggagattatg gaaaattgat
      241 taattatgtt gggattgttc gtggagctag ccaacgtcta actaaactag agatgaatca
      301 tcaaccaaat gatgaattaa tagaatatat tgatgaaatc ctacaggagt tgattacagg
      361 gcatggagat tatggtttag taatcactga ttgtaatgaa tacaatgagg acttactttt
      421 attagaaaaa aagtgggaag atttaaatat tgaaattaaa aaagtaagaa tgaaagaaca
      481 gaataaccag ttattaagta taagtgaaga gtttttttct cttgcaaatg atacagtgtt
      541 taaaatagaa aacttttcta aagaaaaaag taattactta atgactttaa taataattat
      601 atctattata ggtatattag catgtatcat attgatttta caatatagta aaaaaatggt
      661 tagactggaa aaattaaatg tagatttaaa aaatattgca tataaagatg aactaacagg
      721 agtaaatact atagaaaaat ttaaattgga tgcaaatcaa aatatttgta tacataagga
      781 taagaaattt gctgtattct atattgattt tgaaaacttt aaatacatta atgatatatt
      841 tggatatgat tatggtgaca tgatactgaa aagatatgct aatcttatga tgaatgacat
      901 agggaaatat gaaatatttg caagagaaat tgcagataga tttgttgcgt taagatgcta
      961 tgtagataag gaagatttag tggtaagaca acgagatgta gacaatgaac taattaatat
     1021 aacaaatgaa attaaaaata aacatagtat tactgttgtt agtggaattt gttgtattga
     1081 agatgtaaat gaaaaattaa gtatagatgg tttaataaat agagcaaatt ttgcacaaaa
     1141 aactgttaag aataaaccag gtactaatta tgctttttat aatgacagta ttcgtaaaaa
     1201 aatgattgaa gaaaatacta tcaagagtag aatacatgaa gctattgaaa aaagagaatt
     1261 tatagtttat ttacagccta aagttaattt acatagtcaa aaaataaatt gtgcagaagc
     1321 tttagttcgt tggcttactc cggataaagg tattatatcg ccagcaatat ttattccagt
     1381 acttgagaag aattttttta ttgccttagt agataagtat gtttttgagg aggtttgtaa
     1441 atggattaga aagaggttag atgaaaataa accttttgtt cagatatcag ttaatgtttc
     1501 aagaatacaa ttttataaca ctaaatttgt agaaacttat tccaatatac aaaataaata
     1561 caggatacct aaaaatacta tagaaattga atttacagag agtgttgctt ttgagaatca
     1621 aaaacatctt ttggaaatta tacatgattt gcatgaaaat ggatttactt gttctttaga
     1681 tgattttgga aaaggttatt cgtctttaag tgttttaaaa gatttaccat ttgatgtttt
     1741 aaagcttgat tctatgtttt tcaaggctag tttagataaa gataaagaga aaatagtaat
     1801 aaaaaatatt gtacatatgt taaaagaact taatatcaca actgttgctg agggcattga
     1861 atgcaaggaa caagttgaat ttcttaggga tattgggtgt gatttagttc agggatttgt
     1921 attttataaa ccaatgccaa tattagaatt tgaaaaaata ttggataaag agtttgttta
     1981 taatagctaa aatgtacttt ataaaatatg aactaattaa gtaaaattat tttgtactga
     2041 ataaattttt actttattta cagaataatg attatttaca gaaaagcgcg aaaagtgtat
     2101 tcctttaagt gtgggatgga tagcacaaat agaggagaaa aaatattggc catataagaa
     2161 tgcataataa tataaatatt tgaaaggtca gcattatata aaaattaaaa agaaaataaa
     2221 taatttttct ataaaaaaga gactatgaga aaatttactc atagtctcct aaataatgta
     2281 tttattttct tcctgcaacc cattttaatc cccaatgaaa ttcagtatcc atttctctat
     2341 gacctttgaa gtatttaact actttttcaa cactgaatgt ctcatcattt acattttcta
     2401 tcaatgctat tgaacacata ttatagccat ttttatgttc atcaagtttg acaactaagt
     2461 ctgaatcttg tttttgtttg attgttacaa ttccatcagc ttcagaccag ttagctacac
     2521 cttcgtatat aaaagcataa ataagtattc tttttatatc ttttatttta ttaccattta
     2581 ttcttaaatt ttctccttgt gtattacttc ctgttctgtc atctccatct aattgagcaa
     2641 aaggtggatt gtctaaacta ccaaaggcat ttcctaatgc ttgaacagct ccttttacac
     2701 catttttcat ctcaaatagg caacccaaat ctaaatctat attactgctt cttaaagagc
     2761 caaaaaaacc ctttttttgt actttttgat tccaatttaa attaactaag atttcaccta
     2821 aatcgctatt ttcctttttt gtaaggttta ttttttgtcc cttttttaat tctatagcca
     2881 tagtaatctt cctccacaaa catagattta tttaaatggt agcacttaat aagtcaatat
     2941 tcaacaagat tagaataata atattaaaat tatactaaat atactaaaaa gtaggaatca
     3001 caataccttt gtatttttct tcaattaagc ttcttgcttt atctgatgta agaacatctt
     3061 gtagaacttt aatcttttca ctgtctttat cttcagttct aaatgctaaa agatttgcat
     3121 actcgtcatt tataggagct gtataaattc cgtttttctt ggcatcaaga ccagcaggta
     3181 cagcataagt agtatctata aatgcagcag tcacatctgg aagagaagat ggaatttgag
     3241 caatatctaa ctctttgaat tgtattccct ttggattttc tattatgtcc ttaggactaa
     3301 ctaatccatc ccctttaggt aacgttaaaa gtccaatact ttcaaggtat ctaaggttct
     3361 tagaacaaga agatgggtta ttgcttattg caattgtatc tcctttttta aattcatcaa
     3421 cagattttat cttataagag taaagtattg ctgggcatac atatagtttt ttaccagcca
     3481 ttattttgta acctttggcc ttaacagctt cttttagata aggttcatgt tgaaatagat
     3541 ttgcatcaat ttcaccatta tttaaagctt cgtttggaag tatgtaatca tcaaagtttt
     3601 taacttctaa agtatatcct ttttctttaa taagtggttt taattcttct aataactctc
     3661 ctcctggaac taaagtagca ccaacaacaa tttttttatc atctttagct ttagaacatc
     3721 caaccacagc taaagttaat actagacata ataacaaaca taataatttt ttgaatttca
     3781 tataaattcc ctcctgaata taattgatta gttaaaaata aaataccatt ctttatcaca
     3841 ctgcaaaata aaaataagcc tgagtttaaa ctcaggcttt aaaagctaat ttagactcat
     3901 ctttcagtat aatactgcag gatttagcac caatgtatat aaaaatatac tggttgctgg
     3961 gtttcatagg gccagtccct tcaccactct ggataagaag tatcaatttt taattatgtt
     4021 ataaatatta tcatgatttt ttacgtctgt aaacataatt ttataaatat tttaaaatct
     4081 ttaatgtatt ttatttaaca cctaaaattc cagaaactag aatatcagta tcagcaccta
     4141 agaaatagtc atttatttca atagaatcca gagtatttct caaagcattt tcttcatgcc
     4201 ttataccaat aagtttattt tctacaaatg caacatcaca ttttccaaaa aaatctccaa
     4261 aaaattttat actagttatt atgcctttat caacatttaa attaaactca acatttccac
     4321 cagaatattt taattcattt gaaagagtat attttggtga attaccaaaa ttccaatccc
     4381 aagtttcgta ttttgattta gtaagctttt ctatttgttt tatatcatgc tcactcaaac
     4441 aataattagt attatcagta tttgttttag aaagataatc cattatagca tctttaaatt
     4501 ctaatatgtc catttttgaa tgtaaatgtt cacttatatt ggttacacga gattttacag
     4561 attttattcc ttttccttca aattttatag gtttaacctt taatgctttt gagaggtcat
     4621 ttatttcaga tgaaaataaa agagtaccat gatgcattac tttgtttttg tagttgtatt
     4681 gagcatttcc agaaaatttc ttgccatcta taagaaggtc atttcttcca gaaaattcgg
     4741 cattgacatc tagtgtttta agtaaatcta ttatgggctg agtgaatctt ctgaaatcac
     4801 taaaattatt attgttacaa gctataaatg taaaatttat atttcccaaa tcatgaaata
     4861 cagctccacc accagactgt ctcctaacga caggtataga atattctttt atatattcta
     4921 aatttatttc agaaagtgtg ttttggtttt ttccaacaat tatagaaggt tcattcctcc
     4981 ataaaataaa taagtcttca tcaaaatttt ttagaagata ttcttccatt gctaaattaa
     5041 aataagggtt tgttttttca ttataaatta ataacattaa agtagttcct ccatagaatt
     5101 gataaaattt ttattatgaa tacaattaat tttataattc gacaataata taaaaactcc
     5161 tttaaattag aaattttaaa aactaccttg caaagtttaa ataaataata tatactcaaa
     5221 atatgtttaa taaaagtgaa tagaagggta gaggcgcaat aatctatcag tactaataag
     5281 gagttaagca cagagattta ttaggaaagg agatattgcc gaagttttat aatcgatgct
     5341 ttaagattat attgctgggg gtacatagaa aatatgtaca actgtcacag gtttatttgt
     5401 ggtgagctat catttaagga attatattat atttattttt actattgttg atatattgta
     5461 atgtttaagc cttgagtgtt taccactcaa ggctttttta gttttattgt aacataatac
     5521 cctgtacagt tcacaaaatt aaaaggagag ggagatttat atgaggaaca aaaaaattaa
     5581 tattattttt ctgacgacaa tcatgttcat aatgtcaaca gtaatggttt ttgcagaaga
     5641 agatatagac actattgcac tagccaatgc agaaaaattt gggattttaa ctttaatacc
     5701 accattagtt gctataatac ttgcatttat aactaaaaac gtaataattt ctttacttat
     5761 aggtattctt tctggtagtt ttataattaa agctagtggt attaatgttt ttgcgacatt
     5821 tatacaggca tttttagatt tagtagacag agctttagta tctttagcag acccttggaa
     5881 tgcagggata atactacagg ttttggcaat tggaggagta ataaatcttg ttgctaaaat
     5941 gggaggagct aaagctatag ctgaagcact tgctaaaaga gcaaagtcag caaaagggac
     6001 acagcttata acatggtttt tggggctttt agtattcttt gatgattatg ccaattcact
     6061 aatagtaggt cctatgatga gacctgttgc agataaaatg aaaatatcga gagaaaagct
     6121 agcatttata atagatgcca cagcagcacc agttgctggt ttagctataa tatctacatg
     6181 gataggtctt gaggttggtt taatacatga tgcttttgaa agtataagta tagatgtaga
     6241 tgcatttggt atatttttaa atacaatacc atttagattt tacaatatat taatattagc
     6301 tttcattgta atatcagcat tattattaaa agaatttggt ccaatgagaa aagctgagat
     6361 aaaatctaga agtagaaaaa ttagtataga ccttgatgaa ggcgtagaag agttggatga
     6421 tttagctcca aaaaatggag taaaattgag tgtatggaat gctataatcc caataggaac
     6481 attgataata gttgcattag catcatttta ttatagtgga tatacatcta taatgggagg
     6541 agacgataag gctctgatac aactatttac aaattctcca tattcttttg aagcaataaa
     6601 agaagcattt agtgcatctg atgcatctag agcattattc cagtctgcac ttgttgcaag
     6661 tttagttgct attataatgg ctgtagtaaa aaagattttt acaatatcag aagcaattga
     6721 tgtttggata gatggaatga aaagtttagt aatcactggt gtaatattaa tacttgcatg
     6781 gtcattaagt tcagtaataa aagagttagg tacagctaaa tttttaatac acctattatc
     6841 aggttcacta ccaccattct tattaccaag tttaatattt ggtttaggag caataatatc
     6901 ttttgctact ggaacagctt atggaacaat gggtatactt atgccacttg caattccatt
     6961 agcatactca ttaaatcctg atatgtctta tgtaatagta agtactagtg cagttttaac
     7021 aggtgcaata tttggagacc attgttctcc aatttcagat acaacaatac tttcttcaat
     7081 gggagctggc tgtaatcata tagaccatgt taacactcaa atgccatatg ctatatttac
     7141 agcagtaatc actattgtat ttgggtatat accagctgga cttggattgc caatatatat
     7201 agtattacca gtggcaatag ctgctatatt tgtaggtatt caaataattg gtaagaaagt
     7261 tgatgaagcg gaaatagaac ttgtagaata aagatataaa taagtttcac cttttattat
     7321 cactattttg ttagttttta gataatttta aaaagttatt gaaaataatg gcgaaatata
     7381 gtaaaataaa agagtgataa gcggatgtgg cggaattggc agacgcacta gacttaggat
     7441 ctagcgccat tggcgtgggg gttcgactcc cttcatccgc actttacagt acttaattgt
     7501 actgaaaaaa actggggttg ctggctttcg gagtgggcta tcccgttttt atttttttgt
     7561 atatataata gattggcact ttagtataag gtgctttttt tataggcaaa aacagtacat
     7621 ttatttctgt tttattatca ttcatctatt agaatatact ttagttaaga atgtgttaat
     7681 aagcagtgtt cacattcttg ataaaatgtg gaataatatg tatttttact aagacggaaa
     7741 tgaaataatt aaaccattaa ataatattat aagatgatga ataagtagat aaatcaaatt
     7801 ttgtaggagg atattatgga aaaatatata tggttgtttc cattactttt tatttttcat
     7861 gatatggaag agataatagg ttttggaata tggcttaaaa aaaataaatc aatgcttgat
     7921 aagaagtatc catttatcag taaagtttat gaaaattata gtactgaagg catggcattt
     7981 gctgtgtttg aagaatttat tttatgcatt attttctgta tacttacagt tataacagag
     8041 aatcagtatg tatatctact atggttaggt agttttatag catatacctt acatttggta
     8101 attcatatag ggcagtcaat cataataaga aaatatatac cttcacttat taccagtatc
     8161 atatgcttac caataagcat ttggtgtata agtaagagta tttatatagt tgattgtgaa
     8221 atgagtacaa caattctgta cagtataatt ggcattataa ttgtagcatt gaatttaaag
     8281 tttgcacagt cgctgattgg aaaatttaca aagtggatgt caaacatata aattttagtt
     8341 tattggacag ttcaaggaat cagaaattgg aattttatag tattaatatt ttatggtatt
     8401 aatataatgt ataaaatttt atagcaatgt atttcataag tataaagtga ctagaatttt
     8461 aagaaatata caaattctat aaaaactaaa taatttcaag tatattttaa aaatcttatt
     8521 tttcacaagt ttaaaattgt aaacgatttc atcaagtcta tgaatatact tttaacatgt
     8581 tatagtaaat ctcaatataa atttacattt ggttctttgt gtgttatttt aataataaat
     8641 taagtgaaaa tgttatttta cattaatata taatatcttt gcataagagt gaataatata
     8701 aaagagaaat aattgataat aaaaacttaa caaaaaaatt aatacataaa aaaaaataaa
     8761 tataatgtgt tgacatttaa ttgaaatatt catacaataa aattgaaaat aaatatttcc
     8821 aaatgagatt agcgagagag tcctttatac gaaaaggcgc cgaagaagaa atttaagaaa
     8881 tgccaatttt tagagaagct ttcaggcaaa aatatcgcta attgatggga ctctggagag
     8941 acttaagtta agatactata agtcaccgaa gaagcaaaat aatattaaat tattaaatct
     9001 ttcaggtata caaacaggga tatataggct aggattattt tccatgctga tatatctttt
     9061 ttttatgaaa aagtacagga aataatatca aagttactta aatacagtag gtctaatttt
     9121 ctagggaggg ttatatatga atgaattgaa aagagtatct ttgtacaata tacacaaaga
     9181 gcttggagca aaacttgttg aatttgctgg ttgggaaatg cctttagaat atgagggtat
     9241 aaataaagag catgagaagg tcagaaaaag tgcaggtata tttgatgtat ctcatatggg
     9301 agaggtacag ataaagggag cagagtcaga aaaatttata caaaacctag ttacaaatga
     9361 tataagcgca ttaaagataa atgatattat atatacacct atgtgttatg aaaatggtgg
     9421 agtagttgat gatttattaa tatataaatt tggtgaagaa gattatttat tagtaataaa
     9481 tgcaggtaat atagataaag atgtagcatg gataataaaa caaagtgaag gatacaatgt
     9541 agatataaaa aatatatcta gtgaagtgag tcaattagca attcaaggac ctaaagcaga
     9601 agaaattctt caaaaaatca cagatataga cttaaactca ataaaatttt ataagtcaat
     9661 tccatcaaca aaagtatgtg gttgtccttg tttagtttca agaacaggat atactgggga
     9721 agatgggttt gagatatact gtaaaaataa atatgtagaa ataatttgga atgaagttct
     9781 aaaggttgga ggagaagata tatgtccagc tggtctaggt tgtagggata ctttgagatt
     9841 tgaagcagct ctacctctat atggacatga aataaatgaa cacatatctc caattgaagg
     9901 tggtttatct atctttgtga aaacaaataa agaatcattt attggcaaat caattttatc
     9961 aaaagaaaaa gaaagtggag ctaaaagaaa gttagtagga tttgaaatgc aaggtaaagg
    10021 aatgcctaga aatggatatg atataaggat tggtgataaa acagttggat ttgtaacaac
    10081 aggatgtgca tctccaacga caggaaaaat tttaggtatg ggaattatag attctgaata
    10141 tgcaaaagta ggaaatgaaa taggtatagc aatcagaaaa aaagttgttc ctgctgtgat
    10201 agttaagaaa cctttttata aaaaacaata taaaaaagat aatattatat taaacaaaga
    10261 aaataaattt tcatatatac ctgctacaag tgaagataaa tcaaaaatgt taaaagtagt
    10321 tggattaaat tcggttgatg aattattttc agatatacca gaagaagtaa aattaaaaag
    10381 agatttaaat ttggaaattg gaaaatctga attagaggta agcaaaatag taaaaagatt
    10441 gtcagaagaa aatttaagtt tagaagactt gacttgtttt cttggtgctg gtgcatatga
    10501 ccattatata ccatcaatta taaaacatat aacttcaaga tctgaatttt atacagcata
    10561 tactccatat caagctgaaa taagtcaggg aactttacaa gtagtttttg aatttcaatc
    10621 tatgattgca gaaattacag gtatggaaat tgcaaatgca tcaatgtatg atggagcaac
    10681 tgctgcaata gaagcatgta taatggcaat gaatcagact agaaaaagta aaatagtagt
    10741 gtcaaaaact attcatcctg aaactttgtc aatattaaga acttacttac aatataaaga
    10801 ttgtgagata gtagaaattg acttttgtaa tgaatatggc actacagata tagaaaaatt
    10861 aaaagcttct gtagacaaag atacagcatg tgttcttata caaactccta atttctttgg
    10921 aattattgaa gaaatggaag aaatagaaaa gataactcat gaaaataagg ctatgttaat
    10981 tatgagtgta gacccaatat cattaggagt tttaaaaaca ccaggtgaga taggtgcaga
    11041 tattgttgtt ggagaagctc aatcacttgg aaaccctctt aattttggag gtccttatgt
    11101 aggattttta gctagcaaat ctaaatatac tagaaaaatg ccaggaagaa tagttgggca
    11161 aagtttagat gtagaaggta aaattgcata tgtattaact ttacaaacta gagaacagca
    11221 tgtaagaaga gaaaaagcaa cttctaatat atgttcaaat caagctttaa atgctcttgt
    11281 agcttcaata tatatagcta ctatgggtaa agaaggattt aaagaggttg gtatgcagtc
    11341 tatgaaaaaa gcacattata cttacaataa gcttgtacaa actggaaaat ataagccaat
    11401 atttaaaggt aagtttttca aagaatttgc tgtacaagga aatcttaata tagaaactat
    11461 aaatgataaa ctcttagaag aaaatatatt aggtggttat aatttagaat acaattatcc
    11521 agaattaaaa aattcaactt tgctttgtgt gactgagaaa agaagcaaag aggaaataga
    11581 taagttagtt ggaataatgg agggattata atgaaagaat acaatagttt gttgatagat
    11641 atatctaaaa aaggtaggaa agcatactca cttccaaagt tagatataga tgatataaaa
    11701 atagaaaata tgattgacca agatatggca agacaaagtg agttgaattt gccagaagtt
    11761 ggtgaattag aattagttag acattataca ttattatcaa ataaaaactt tggagttgat
    11821 acaggttttt atcctttggg ttcttgtact atgaaatata atcctaaaat aaatgaagat
    11881 atggcagcac ttccaaactt tactggaatg catccatacc aatcttcgga tacagctcaa
    11941 ggttctcttt ctttgatgta tgatttatca agaagacttg cagaaattac aggtatggat
    12001 gaagttacac tacaaccttc agcaggttct catggtgagt ttacagggct tatgattata
    12061 aaagcatatc atgaaaatag aggtgaccat aagagaacca aagtgataat tccagattct
    12121 gcacatggaa caaatccagc aagtgcagct atggcaaact ttgatgtgat tcaaatagca
    12181 tctgataaaa atggagctgt agacataaat gcattaaaag aggttttgaa tgatgaagta
    12241 gcagctctta tgcttactaa tccaagtact cttggtttat ttgaaaaaaa tataaaagaa
    12301 atagctactc ttgtacatga agcaggagga cttttgtact atgatggtgc aaacatgaac
    12361 gctattatgg ggataacaag acctggagat atggggtttg atgtggttca tcttaatctt
    12421 cataagactt tttcaactcc tcatggaggt ggaggtccag gagctggtcc tgttggagtt
    12481 aaaaaagaat tagtaccttt tttaccaata ccagtaattg aaagaaaaga agataagtat
    12541 gtactaaatt atgatagaga aaaatctatt gggaaaatta aaaatttcta tggtaatttt
    12601 ggagttcttg taagagctta cacatatata ctaactatgg gaagggatgg acttaaagag
    12661 gctagtgaaa tggcagtttt aaatgctaat tatataaagg aaagtataaa agatgattat
    12721 attttgccaa tagatacctt gtgtaagcat gagtttgtat taggtggatt gccaagagga
    12781 gaggccaata taaaaactat cgatattgca aaaagactac tagattatgg ataccatcct
    12841 ccaacaatgt actttcctct tattataaat gaggcactta tgatagaacc tacagaaagt
    12901 gagagtatag agacattaga tagttttata gaagctatga aaagtgttgc taaagaggca
    12961 aaggaagaac cagaactatt gaaaactgct ccacataata ctttggtcaa aagagtagat
    13021 gatgcaaggg cagtaaagaa acctatacta acatggtcac aaagataaat agttaaaaag
    13081 tagagattta aagtataaag agaattaaat ttgtaagaaa aagctatcta acttaatcta
    13141 gtttagatag cttttttgta tattagtatg tatggtattt tctatataaa gttttaaatt
    13201 atattagtgc ttatatagaa tattgatatg atgattatgt atagatatta ataagaattg
    13261 ttgaatttat cttgccttaa ctttatttac tcttttatat taagaaaaac ttaactaaaa
    13321 tatagcgaaa ttcataaaaa tttaggaaat cttaagaatt ataatattta aatctttaga
    13381 tttatatttt attatataaa ctatagaata ataaaataaa aaaattgaga attttataat
    13441 atatttgtag atttgagtat aaaaaagtgc taagttgaaa ggagtaaaat atggataagg
    13501 ttaatttaga aaggtatagt tgtgatataa atgttggttt aaataaaaat caacttcaaa
    13561 gtagaataga tgataattta attaatgcat ttgataatgg aaaaaccaaa acatataaac
    13621 aaatttttaa agacaatata tttacacttt ttaatctcat aaatattgtt ttagtctgtt
    13681 tattagtttc agttggttct tttaaaaata ccttatttat atttattgct gtaattaata
    13741 ctattatagg agtaatacaa gagataagag ctaaaagatt attggacaat ctatctgtta
    13801 tagtttcaaa taaggtatct gtcataagag aaaaagagaa aaaagagatt gatgtacaag
    13861 aattagtatt agatgatatt atgattttaa aaacaggtaa tcaaatttgt gcagattcaa
    13921 aagttttaaa tggaaaatta gaggtaaatg agtctttggt tacaggtgaa tctgatatta
    13981 ttataaaaaa taaaggcgat tttttatatt ctggaagttt tgtagtttct ggagaagctt
    14041 ttgtaagagt tgaaaatata ggaaaagata actatgcaaa taagatagct aatgatgcaa
    14101 agatttctaa aaaacataat tctcaactta gaaattcatt aaatgcaata ttaaaaattg
    14161 taggtataat aataattcct ttaggaatta tattgtttgc aaaacaacat tttggaaatg
    14221 gagattctat agctacatct gtagtaggta cagtggcagc tgtaaatggt atgataccag
    14281 agggattaac attacttacg agcattgctc tagctgtagg aactataaaa cttgctagtc
    14341 ataaaacttt agtgcaagaa ctgtactgtg tagagactct tgctagagtt gatacactat
    14401 gtcttgataa gacaggtaca attacagaag gtaaaatgca agttgatgat atagtaatgt
    14461 tggaagaagt ggatattaat gaaataatgg ggaatatatg taattctctc aaagatgata
    14521 atgcaacact aaatgcaata agagataagt ataatgtttt agatacgtat aaagctaaga
    14581 atttaatacc attttcttca gaaagaaaat acagtggggt tacctttgaa aataaaggta
    14641 catatttatt aggtgcattt gagtttatat ttaaggaaaa aaataaaaaa cttaggatag
    14701 aagttgaaag atattctaaa aaaggaaaca gagtaatagt tttagctcat agtgattctt
    14761 atatggaaga taataatatt cctgaagata taaagccact agcttttata ttgatattgg
    14821 ataaaattag agatgaagct aaggaaactt tagaattttt ttacaatgaa ggtgtagata
    14881 taaaaattat atctggtgac aatcctataa cagtaagtga ggtagctaga aaagcaggtc
    14941 ttaaaacagc tagtaatttt attgatgcaa caacacttaa aaatgatagt gatatataca
    15001 aagcagttga taaatacagt gtttttggaa gggtaagccc acaacagaaa aaacaaatga
    15061 ttttagcatt aaaaaaacaa aatcatacag tagctatgac tggagatggt gtaaatgatg
    15121 tactagcact taaagaagcg gattgtagca ttgccatggc atctggaagt gatgttacta
    15181 agaatgtgtc taatttagtc ttattagatt caaactttgc atcaatgcca aaggttgtta
    15241 tggagggaag aaaagtaatt aataacattc aaagagcatc ttctttgttt ttagtaaaga
    15301 caattttttc attcttcttg tcgttattaa cattattctt cttcagtaga cagtatccat
    15361 ttgttcctat acaattatca ctcattgggg ctgtaacagt gggtatacca tcattcttct
    15421 tagctcttga agcaaacaaa agtagaattt caggaaattt tttactcaat atattaaaaa
    15481 atgctttacc aggagcacta tgtgtgacag taaatgtaat tattgtttac aatatagttg
    15541 aatatttagg ttatagttca agtgtatttt ccacaatgtg tgctatatta actggtgtat
    15601 gtggtctttt aatattgaga caacaatgtt tgccatttaa taaagaaaga ttatttttaa
    15661 tagtaagtat gactattgca tttgttattg ggattctatt tttaggtgga ttattctcat
    15721 ttgtagcatt gagtagagaa ttaattctca taactatggc attagcaata ttaatgccaa
    15781 ttatgataaa agttatgtta tttgtcttag atgagatatt agagacaatt gttccagatt
    15841 taaattaatt ttaaaatgat ataaataaga gcagttttag ttgaactgct ctttttagat
    15901 gtgtttaatt ttatgtaaga atatgtattt ttattaatca gctatatatg agcaacagta
    15961 gtcaacaact tcattaacct ttaagtcaaa acttgaatca ctaggaacat taaatttttg
    16021 cccttcttta taagttacaa actcattgct tccaggcagt tttacatcca tacttccagc
    16081 aagcatctcc attatttctt cttctctagt agaaaaagtg tattctcctg gcaacattaa
    16141 tcctaaagtt tttctttctc cattaggcag tattattact ctgctactta ccttaccatc
    16201 aaaataaacg tttgccttct ttactgcagt aacattctta aattcactca ttagataaaa
    16261 cctccaaaat tatttgtaca tatatattat ataaaaataa tgtaaaaaat gctatatatt
    16321 tacaaaaaaa tttagtattt attaaaaaaa ttaataagaa taaaaaagag gtgaaattgc
    16381 ttaaattgat aaaaaatata aaataaattg caaagtaagc aagaaaagaa taagttgaag
    16441 agttatatat tataaatatt aaaaatttta gtattaataa aaaagatatt acattaatat
    16501 gaaaatatag gagattagaa gtgtttgatt aaaaaatagc tacacacact tgtgacttta
    16561 gtcatgagtt agtagctaaa atagtcagca tatagggaaa cttatatgta gagataggat
    16621 agaacctatc caatactact tgaattgcta aaaaccccta aagctaacca aactaaaaca
    16681 taaggatgaa ataaacctaa gtgtgaaagt tacgaaagta gaaaaaattg gttagatggt
    16741 ataaggttaa accctaagta ctttaaaatg ggcaatcagc aggtaagctc cgaataggag
    16801 aaacttcaac gactattcct cttgagggaa gtacactaca agctattggt agtggaagtg
    16861 ggtagacctt aacggataat gccgaaggat aagatatagt ctgtgcttac gtgaaagtgt
    16921 aagaagttca taagagaact gcataggtag tagcgaactt atgtgaacga caacctcaaa
    16981 aatgataaaa gttaagactt acggttctta tatatgttat ttaaaaataa atataaaaaa
    17041 acttcaaaat acttgtttta agtaattgta tattttaata ttatgatagg ttatgaaaaa
    17101 tggaagttac tcatggtaga ggatatgcat atagtatgtc aaatatagac agccagtatt
    17161 atggggagac atctttggaa tcctagttat ttcgttgcta ctgtatcaga aaatacagag
    17221 gagcagataa gaaaatatat taataatcag aaaaaacaag taaagtgagg tggtagtggt
    17281 ggaaaaagct tataagttta gaatgtatcc aaataaaaaa caacaagagt taattaacaa
    17341 aacttttgga tgttgtagat ttgtatataa taagtatctt gctaaaagaa tagatgttta
    17401 taaaaacaat aaagaaactt ttacttataa acagtgtagt tctgatttaa ccaatttaaa
    17461 aaaagaatta aaatggctta aggaacctga caaattttca ttacaaaacg ccttaaaaga
    17521 tttagataat gcatataaaa aattctttaa agaaaaa
//
LOCUS       DAEPIN010000075        17511 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.075, whole genome shotgun sequence.
ACCESSION   DAEPIN010000075 DAEPIN010000000
VERSION     DAEPIN010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17511
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.075"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            <1..406
                     /locus_tag="KR250_003024"
     CDS             <1..406
                     /locus_tag="KR250_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0068919.1"
                     /translation="TSSIESYDNLIKLSGLMLFIGIFLSVVFLLCTGSIILFKQLSNI
                     YDDKERYIMLIKLGANNKDIEKIISKQLKVIFLMPLVVGTVHNLFAMSIAQKFIPRSL
                     LVPIIITLVIYFIGYFIYYFLTLKYALNMIKE"
     gene            631..2244
                     /gene="ggt"
                     /locus_tag="KR250_003025"
     CDS             631..2244
                     /gene="ggt"
                     /locus_tag="KR250_003025"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HBH0068920.1"
                     /translation="MKFNAYQHKYSSIRNVVYAKNGAVATSTPLASQAGLEILKKGGN
                     AVDAAVATAATLAVVEPTSNGIGGDAYALVWIEEEKRLYGLNSSGFAPENMELKNYNN
                     MKEMPKYGFGAVTVPGIPAAWSELNKKYGKLSLMECLSPAINYAREGYVVSPNVAKVW
                     KKSYELYEKELIGEEFKPWFDTFSKDGKAPEAGDIFICEEQASTLEEIANTQAESFYR
                     GRLADKIDEYSKKFNGAIRKSDLECFYPTWVEPISTEYKGYKIFEIPPNGHGITVLMA
                     LNILKELELEGNIENVEDIHKIIESLKLAFADSKTYVTDIEHMKVKIQELLSQEYAKK
                     RSLLIDNKEALYPTAGEPYCGGTVYLCTADKDGNMVSYIQSNYINFGSGIVIPRTGIA
                     LHSRGNNFNLDPKHHNVVKPFKKPYHTIIPGFLGKEDKAIGPFGVMGAFMQPQGHIQV
                     LTNMIDFGLNPQEALDAPRWQWIKGKEIEVEPEMPKHIIDSLIEKGHEIKVIHDTVDM
                     GRGQIIFKTEQESYICGTESRCDGHIAVY"
     gene            complement(2377..3228)
                     /locus_tag="KR250_003026"
     CDS             complement(2377..3228)
                     /locus_tag="KR250_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HBH0068921.1"
                     /translation="MKNIISAKELISKLEKNDNLVVIDCRFDLINRTYGIDSYKKGHI
                     KGSFILDIDKDLSSPTKEHGGKNPLQDPLILKEKLEKMGVDNDTTIVTYDDGDLNGAC
                     RLFFQLKHLGLKNVYVLDGGITSFVKEGGELEEKVNIPSCTGKEIRPNINNGLVVPME
                     YVKSKLYKKDTVIIDCRANERYQGLVEPAYSKAGHIPSAKNYFCKDLIKSDFENGSLK
                     DIEFLKKFFKDLNNYDEVILSCGSGISACVNSLALRELDIPHKIYIGSFSDWISYDDN
                     EIKTGQE"
     gene            3854..4747
                     /locus_tag="KR250_003027"
     CDS             3854..4747
                     /locus_tag="KR250_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HBH0068922.1"
                     /translation="MSNKIVSKRQLTDSIYLMEIEAPRVAKSSQPGQFIIIKNDEKGE
                     RIPLTIADYDREKGTVTIVFQTVGASTKKLAMFEENDFVMDFVGPLGQASEFIHEDIE
                     ELRNKKILFVAGGVGSAPVYPQVKWFKEHGLDVDVIIGARTKELIILEDDMKKVAKNV
                     YVSTDDGTYGFNGRVTDLLKDLVDNQGKKYDQAIVIGPMIMMKFMCQLTKELNIPTIV
                     SLNTIMIDGTGMCGGCRVSVGNETKFACVDGPEFDGHLVDFDQAMRRQSMYKTQEGRA
                     MLKLEEGDSHHHSNCGCGGNK"
     gene            4747..6141
                     /gene="gltA"
                     /locus_tag="KR250_003028"
     CDS             4747..6141
                     /gene="gltA"
                     /locus_tag="KR250_003028"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HBH0068923.1"
                     /translation="MDAKKVKVPVREQEPAVRATNFDEVCLGYNKEEAMAEANRCLAC
                     KKPKCVGGCPVGIDIPGFITKIKEDDIEGAAKVIAKSSSLPAVCGRVCPQESQCEGVC
                     ILGIKSDAVSIGKLERFVADWSKENDINLSDTEPKKNQKVAVIGSGPAGLACAGDLAK
                     KGYDVTIFEAMHEPGGVLTYGIPEFRLPKQAVVQPEIDNIRKLGVKIETNVIVGKTIT
                     VDELIEDEGFEAIFIGSGAGLPMFMNIPGENANGVFSANEFLTRVNLMKAYRDDYDTP
                     ISSGKKVAVVGGGNVAMDAARTALRLGSESYIVYRRSEKELPARAEEVHHAKEEGIIF
                     NTLTNPKEILVDENGYVKGMVCIRMELGEPDDSGRRRPIEIEGSEFVLDVDTVIMSLG
                     TSPNPLISSTTKSLDINKKRCLITDENGQTSKEGVFAGGDAVTGAATVISAMGAGKTA
                     AASIDEYLKAKVNA"
     gene            6507..8726
                     /locus_tag="KR250_003029"
     CDS             6507..8726
                     /locus_tag="KR250_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBH0068924.1"
                     /translation="MKKREKVFDGLVFKHILILLILLVLIDLTSTIIYASNSMEITSR
                     NMIEASKRELENYLEVNISLLKALSQDDRFSNGETSLIEKGKLLSPYQKEYNLFMIGI
                     TDTKGNASSTYRENVNSIKDKPSFEKAIKTKQVVVSDIEVSNVTGDKVFIIYVPIVKN
                     NEMIGTIFASFYFQDVNNLISRSNFDDSIKFLMIDKDYTIISHPNKKYVNDKSKMLDL
                     EGNIIGTTKSEILKNINKKCQGDFLSWDNWRLYNVKYTNIKWTNWTLVSKCNIFKNFK
                     NLIVNFMIKLYFYIVIFMILWKLSNAKLIEQLKKLAYYDSLSGIKNKEKFRKDSMYIL
                     KNYYQDNFYLVQLDVNKFKYINEMFGYAEGNKILIHISQVLNNNTNKYEICARMDNDH
                     FILLIACNTEDELLNRLSKINKEICNLNTTNSSKYKIVMSSGIYKITKKDDIKKIDLL
                     IDRANIAAKSKKEKYEHSYSFFNEDTRNRLYKEKRLEDNMNKALEKGEFIVYYQPKYS
                     LDDVNEIEGAEALIRWNSPEFGFISPIDFVPLFEKNGFIVNIDMFVFEEVCKTLNKWI
                     NKGYTPVPISVNMSRVHLYRDNFIENITDLISKYNISPEFIELELTESVVFDNLNILI
                     DIMKKIKEIGFLISMDDFGSGYSSLNLLKDLSFDILKLDRGFLIETTDTKRGKIIISK
                     IVEMAKAIDIKVICEGVETYEQVEFLKEIGCDKVQGYLFAKPMVLDEFEKHLNFKFD"
     gene            8873..9619
                     /locus_tag="KR250_003030"
     CDS             8873..9619
                     /locus_tag="KR250_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0068925.1"
                     /translation="MNILSIRNISKTYCGNIPFKALDKVSLNIEKGEFVSVMGPSGSG
                     KSTLLNIISTVDRQSEGEVVLDGYDVSKLKGEKLAEFRRKQLGFVFQDFNLIDTLTVG
                     ENIMLPLTLEGASIKDMNIQTKSISKFLGIDKILDRKTYEISGGQAQRCAIARAIINK
                     PAILLADEPTGNLDSKSTDDVLKLFTRINKEQKVTTLMVTHEAYSASYSDRVIFIKDG
                     CIYTEIKKSESSNSFYSDILAVLSQIGGVR"
     gene            9619..11499
                     /locus_tag="KR250_003031"
     CDS             9619..11499
                     /locus_tag="KR250_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0068926.1"
                     /translation="MDFRKFAYNNIVRNFRAYLAYFLSSVFSIMIFFVFAVSMFHPII
                     TKSGMQSGSTAFMALMTSELIIFGFSLLFILYSLGNFLKYRLKDFGVLMIIGMSNRQL
                     KKLILIENLIIGFLAVMLGILLGLSISKLFLLYLSKLFYMNLTEFYFPVKAMLLTIVS
                     FMILFLITGPMSLKLLSKKSILELLVGTKKPKKEIKSSKLFGIIGVICLILGYAMILF
                     GNKLKINGGMYTTALLITLGIFLFFSQFTILILKYLKNKEKFYKNKVNMLLISNLIYK
                     LKDNTRLLFLITILLSATLVAFTTTSTLVSSQGEASKNNFPMVYSYFSEHNNEKEYEE
                     LQQIREAIKGYDYKELSFPVLKYNREVLLSVKNYNKLSKELGLDKISSKLEKYEGIII
                     PSSNSQEHINSLKQLKNYSIKNLNIEVKKSKNKIIFPTGLFSKVVVISDDLYNEVKGE
                     FSKINFHGFDYKNWQSSAKITENLKEKHFNLDREYNRSYFLTLPDMYLVELQQSKILQ
                     FMGVFIGVILFIAVFSFLYFRLYTDEIEDNVKYKKLSKIGLSFKNMNKIVSIEIGTLF
                     FIPYFIAIINSIISLLFLNQVLNNSFKLENLGILLLISSIYSLYFYLLKKNYTRKVWF
                     FD"
     gene            11618..13009
                     /locus_tag="KR250_003032"
     CDS             11618..13009
                     /locus_tag="KR250_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBH0068927.1"
                     /translation="MENQQLLGTERISKLLLKYSIPAIIGMLVNSLYNVVDRIFIGNI
                     PGVGPLAITGLGVTMPIMTIILAFGMLIGIGTTTTISIKLGQGKVEEARKLIGNAMTL
                     SVITGIIIMILGILFANKILTLFGASENTLIYAKSYINIILLGTVVNLLSFSLNHSIR
                     ADGSPKISAGIMIVGCLTNIVLDWILIFGFNLGIQGAAIATVTSQALTAILTIGYYIS
                     GKSNLRFSKSNLKLDKKLIKAVFAIGMSPFAMQLAASLVQVISNIALKTHGGDLAIGA
                     MATISSIAMVFLMPIFGINQGAQPIIGFNYGAEKYDRVKKAYLGSLVVATIILCMGMV
                     VVMLFPEAIIGIFNKDPELMNISVNGLRIYLLMLPIVGLSVTGTNFIQSIGKAKMAML
                     LSLLRQVILLIPAVLILPTFLGLQGVWTAQPVSDFIATVITGIVVFRELKRYTPKTEK
                     LNENERLNEITTE"
     gene            13116..14156
                     /locus_tag="KR250_003033"
     CDS             13116..14156
                     /locus_tag="KR250_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HBH0068928.1"
                     /translation="MEFTKKSKLKNMYVNMNVARMLSKINEYKGRQLLYKKQPKEILE
                     NLEKKSLVDCSESTYIGNQRDSSNFNLEKLISNEVTPRSREELSIVEYRDVVKTINSA
                     YESIPISSQTILELHGYLYKFSSTRGGSYKSDNDFIEHNLKPSEFISTDSSVNKVEKA
                     VEEICEAYNTLIEEDEIDILILISAFVLDFILIHPFKEGNIKMARVLILLLLNKNGYE
                     VGRYISLGKIFDDSSYEYYSNLNYLKASIGSEKADMNAWIEYFLETILTAYEKLDDSL
                     NISDKKRQTKTSRIEKIINSTLGYFTKEDIRDLCPDIPEPTINRVFNNLRKQDKIEVV
                     ARGRSAKWKKKY"
     gene            14645..15298
                     /locus_tag="KR250_003034"
     CDS             14645..15298
                     /locus_tag="KR250_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBH0068929.1"
                     /translation="MKKKLLYINVNSKPEDLSSSKTVARKFINKFMEKNKDFEVEEID
                     LYKEHIPRLEYQYFEKRNSIVSEENAKNLDDKDRKELTKIRNLCDQFVNASVYVIAAP
                     MWSLSFPAPLKEYIDCIIQDGKTISFEGNDKPQGILNDIDRSMVYIQSSGGHIPWVLK
                     PVMNKGLNYVESIMKFIGIKKFDELLVDGTGTSEEERQAAIEKASEKIDGIIDGMKF"
     gene            15374..15583
                     /locus_tag="KR250_003035"
     CDS             15374..15583
                     /locus_tag="KR250_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0068930.1"
                     /translation="MREEKSNEKYDCYWCNQENNFCVEIKDNIVMIDDGTGTLKQAVF
                     IGYKQIQINLNCSHCQNLNRIKLNL"
     gene            complement(15723..16289)
                     /locus_tag="KR250_003036"
     CDS             complement(15723..16289)
                     /locus_tag="KR250_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HBH0068931.1"
                     /translation="MMNNELNTIILETLNNADITSNDIPSIDLYMDQIISLIDNKLSA
                     NKRFESDKILTKTMINNYSKEGLIKPVKGKKYTKEQILQMIIIYSMKNTLTIQEIKRI
                     LHGVYEKDNFSEKDLVSCYEKFMLIKENQRKNIPDFIESNFENISINPENKDDLLIAL
                     LSLTSMADQLKNISEKLVDRYFPDITKK"
     gene            16450..17103
                     /locus_tag="KR250_003037"
     CDS             16450..17103
                     /locus_tag="KR250_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HBH0068932.1"
                     /translation="MYQFFLKGRDPISSLTHFIGACLSLLATIILVFQSVTLQETSLL
                     MIVSVSVFGLSLIALYSASSYYHFLKGTPEQELFFRKVDHAMIYVLIAGSYTPICLNF
                     MEKKEGIIFVTAIWIVAFIGIIIKIFWMDAPRWLSTSIYLLMGWAIVFDINAFNSIPK
                     DCLRLLIMEGVSYSIGAIIYIIKKPNISPEFGFHEIFHIFIMIGSLFHFLAVLLYVL"
     gene            complement(17261..17437)
                     /locus_tag="KR250_003038"
     CDS             complement(17261..17437)
                     /locus_tag="KR250_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0068933.1"
                     /translation="MYKYDDYVNLTNDLDLPVEIRNKCVRIVSVSENLEDIIIVHKFK
                     LYVVNEKYIKGIME"
BASE COUNT         6815 a         1830 c         2918 g         5948 t
ORIGIN      
        1 tacatcaagt attgaaagtt atgataactt gataaaatta agtggtctaa tgctgtttat
       61 aggcatattt ttgtctgtag tatttctttt atgtacaggt agtataatct tatttaaaca
      121 attatcaaat atatatgatg ataaggaaag atatattatg ttaataaaac ttggagcaaa
      181 taataaagat attgagaaaa taatatcaaa acagttaaaa gttatatttt taatgccatt
      241 agttgtaggt acagttcata atttatttgc tatgtctata gcacaaaagt ttataccaag
      301 gtcattactt gtaccaatta tcataactct agtcatttat tttataggat attttatata
      361 ttatttttta actttaaaat atgctctaaa catgataaaa gaataaatta tttttaagtt
      421 aaaagaatta gatatatgtt ttgaataaaa taacttattt aagtgcagat agaaaatact
      481 agaattgagt ttaagatatt tatttaaaga taaaattaaa gttaggaaaa agtctatatt
      541 ttatatcatt ttgtatcagt tacattaatt gatgttatat aataaatcta agcagaatat
      601 caaataaaaa atgacgagga gctgaaattt atgaagttta atgcctacca acataaatac
      661 tcatcaataa gaaatgtcgt gtatgctaaa aatggagcag ttgcaacatc aacaccatta
      721 gcatctcaag ctggacttga aatactaaaa aagggtggaa atgctgttga tgcagcagtt
      781 gcaacagccg ccacattagc agtagtagaa cctacaagca atgggattgg aggagatgca
      841 tatgcccttg tttggataga ggaagaaaaa cgattgtatg gtttaaattc tagtggcttt
      901 gctccagaaa atatggaatt aaaaaattat aataatatga aagaaatgcc aaaatatggg
      961 tttggagcag taactgtacc tggaatacca gcagcttggt cagaattaaa taaaaaatat
     1021 ggtaaactta gcttaatgga atgcttatct ccagcgataa actatgcaag agagggatac
     1081 gtagtatctc ctaatgtagc taaggtatgg aaaaaatcat atgaactcta tgaaaaagaa
     1141 cttattggag aagagttcaa accatggttt gatacatttt ctaaagatgg taaagctcca
     1201 gaggctggag atatatttat atgtgaagaa caggcaagta cattagaaga gatagcaaat
     1261 actcaggctg aaagctttta tagaggaaga cttgctgata aaatagatga atattctaaa
     1321 aaatttaatg gggctataag aaagagtgac ttagagtgtt tttatccaac atgggtagaa
     1381 cctataagta cagaatataa gggatataaa atatttgaaa tcccaccaaa tggtcatgga
     1441 ataacagttt taatggcact taacatatta aaagaattgg aattagaagg aaatatagaa
     1501 aatgtagaag acatacataa aattatagag agtttaaaat tagcttttgc agattctaaa
     1561 acatatgtaa ctgatataga gcatatgaag gtaaagattc aggaattact aagccaagag
     1621 tatgctaaaa aaagaagttt attgattgac aataaagagg ctttgtatcc aactgcagga
     1681 gagccatatt gtggtggtac agtatacctt tgtactgcag ataaggatgg aaatatggtt
     1741 tcatatatac agagcaatta cataaatttt ggttcaggaa ttgtgatacc aagaactggg
     1801 attgctttac atagtagagg aaataatttt aatttagacc ctaaacacca caatgtagta
     1861 aaaccattta aaaaacctta tcatactata atacctggat ttttaggtaa agaagataaa
     1921 gcaataggtc catttggtgt aatgggagct tttatgcaac cacaaggaca tattcaagtg
     1981 ttaacaaata tgattgattt tggattaaat ccacaagagg cgctagatgc tccaagatgg
     2041 cagtggataa aaggaaagga aatagaagtt gaaccagaga tgccaaagca cataatagat
     2101 tcattaattg aaaagggtca tgaaataaaa gtaattcatg atactgtcga catgggacga
     2161 ggtcaaataa tatttaaaac agaacaggaa agttatattt gtgggacgga atcaagatgt
     2221 gatggtcata ttgcagttta ttaatattat caactaaaat atatataata aatttaaaga
     2281 gtatatgaaa taattagtaa aggcgaaagt ctacaatgac tgacgccttt acaaaattta
     2341 agctatcata tgctgatttt atatagatta tgagccttat tcttgaccag ttttgatttc
     2401 attgtcatca taactaatcc agtcactaaa gctaccaata taaatcttgt gaggtatatc
     2461 cagttctcta agagcaagac tgttaacaca agcagatatc cctgaaccac aagataaaat
     2521 tacttcatca taattattta aatctttaaa gaattttttt aaaaattcta tatctttaag
     2581 tgagccattt tcaaagtcac tttttattaa atccttacaa aaataatttt ttgcacttgg
     2641 tatatgacca gcttttgagt aagcaggttc aactaaaccc tggtatctct cattagcacg
     2701 gcaatctatt attacagtat cttttttgta taatttagac ttaacatatt ccattggaac
     2761 aactaaacca ttgtttatat ttggtctaat ttccttacct gtacaacttg gtatattcac
     2821 tttctcttcc aattctccac cttcctttac gaaagatgtt attcccccat ccagtacata
     2881 gacattttta agccccaaat gttttaattg gaaaaataat ctgcaagctc catttaaatc
     2941 tccatcatca taagttacaa tcgtagtgtc gttatcaaca cccatttttt ctaatttttc
     3001 ttttagtatt aaagggtctt gtaaaggatt tttaccacca tgttcttttg taggagatga
     3061 taaatccttg tctatatcta agataaagct gcctttgatg tgtccttttt tataagaatc
     3121 aataccataa gttctattga taagatcaaa tctgcaatct attacaacta gattatcatt
     3181 tttttctagt tttgatataa gttcttttgc tgaaataatg tttttcaaag aatgtacctc
     3241 ctgtgtttat attatcttta ttttaattta tattaattat taactttata aactaatatt
     3301 attataattt attacataag taaataaatc tttagaaatt gtaaaaattt tatataaaaa
     3361 ttcaaataat taatgaatat tgaaaatttt agatatataa taaaataaga gtatatttta
     3421 tacaatatga aagctggtat tgcaatagat taaagcctta tacacgttta gtttttaaag
     3481 aaattagtgt tattatgaaa acctaagtta atttattaaa taaataattg aataaaaaaa
     3541 ttttattatg tatttataaa atttttgtta caaatttggc ataatatagt atactttaag
     3601 tgtgtattag gttaaatcta taacaaatta gacgtatgat atagacttag tgtttattgg
     3661 catttacaag aaatttgtta atatcaacta tttgtagttt gggcagttat aattattata
     3721 ggggggatgt ccaatacaaa acaatcaacc aagaactaat attatattat aaagagttat
     3781 agttttgtgt gtttatgttt ttaattaaat ttaaagtggg aaaaatatat ttgttagaat
     3841 ggagaatagc gagatgagta ataaaatagt tagtaagaga caattaacag atagtattta
     3901 tttaatggaa atagaggcac ctagagttgc aaaatcttct caaccaggtc aatttataat
     3961 aattaaaaat gatgaaaaag gagagaggat tcctcttaca atagctgact atgatagaga
     4021 aaaaggaaca gtaactattg tattccaaac agttggtgct tcaactaaaa agttagcaat
     4081 gtttgaagaa aacgattttg taatggattt tgtagggcca ttaggacaag caagtgaatt
     4141 tatacatgaa gatatagaag aattaagaaa taaaaagata ttatttgtag ctggtggagt
     4201 tggttctgct ccagtttatc cacaagttaa atggtttaaa gagcatggat tagatgtaga
     4261 tgtaataatt ggtgcaagaa ctaaagaatt aattatatta gaagatgata tgaaaaaagt
     4321 agctaaaaat gtatatgtat caacagatga tggtacatat ggatttaatg gaagagttac
     4381 agacctatta aaagatttag ttgataatca aggtaaaaaa tacgaccaag caatagtaat
     4441 tggaccaatg ataatgatga aatttatgtg ccaattaact aaagaactaa atatacctac
     4501 tatagtaagt ttaaatacaa taatgataga tggaacaggt atgtgtggtg gttgtagagt
     4561 tagtgttgga aatgaaacta agtttgcatg tgtagatggt cctgaatttg atggtcattt
     4621 agttgatttt gatcaagcaa tgagaagaca gtctatgtac aagactcaag agggaagagc
     4681 tatgctaaaa cttgaagaag gcgatagtca tcatcatagt aattgtggat gtggaggtaa
     4741 caaataatgg atgctaaaaa agtaaaagta ccagttagag aacaagaacc tgctgtgaga
     4801 gcaacaaatt ttgatgaagt atgcttaggg tataataaag aagaagctat ggcagaagct
     4861 aatagatgtt tggcttgtaa aaaaccaaaa tgtgtgggtg gatgtccagt aggaatcgac
     4921 ataccagggt ttataacaaa aattaaagaa gatgatatag aaggtgctgc taaagtaata
     4981 gctaagagca gttcattacc agctgtttgt ggtagagtat gcccacaaga aagtcaatgt
     5041 gaaggtgtat gtatacttgg aataaaatca gatgctgtat caataggtaa attagaaaga
     5101 tttgtagcag attggtcaaa agaaaatgac ataaatttat ctgatacaga gcctaagaaa
     5161 aatcaaaaag tagcagtaat aggaagtggt ccagcaggtc tagcttgtgc tggtgattta
     5221 gctaaaaaag gctatgatgt aacaatcttt gaagcaatgc atgagccagg tggagtttta
     5281 acttacggaa tacctgagtt tagacttcca aaacaagctg ttgttcaacc tgaaatagac
     5341 aatataagaa aattaggtgt aaaaatagaa actaatgtaa ttgttggtaa aacaattaca
     5401 gtagatgaac ttatagaaga tgaaggattt gaagctatat tcataggttc aggagctggt
     5461 cttccaatgt ttatgaatat accaggggaa aatgcaaatg gagtattttc tgcaaatgag
     5521 tttttaacta gagtaaactt aatgaaggct tatagagatg attatgatac acctattagt
     5581 tctggtaaaa aagttgctgt agtaggtggt ggaaatgttg ctatggacgc tgctagaact
     5641 gcattaagat taggttcaga atcatatata gtttatagaa gaagtgaaaa agagcttcca
     5701 gcaagagctg aagaagttca tcatgcaaaa gaagaaggca ttatatttaa tacattaaca
     5761 aatcctaagg aaattttagt agatgaaaat ggatatgtta aaggtatggt ttgtataaga
     5821 atggaattag gagagccaga tgattctgga agaagaagac ctatagaaat agaaggttct
     5881 gaatttgtac ttgatgtaga cacagttata atgtcacttg gaacaagccc aaatccacta
     5941 atatcttcta caacaaagag tcttgatata aataagaaga gatgcttaat aacagatgaa
     6001 aatggtcaga cttctaaaga gggtgtattt gcaggtggag atgctgttac tggagcagca
     6061 acagtaatat ctgcaatggg tgctggtaaa acagcagcag catcaataga tgaatattta
     6121 aaagcaaaag ttaatgctta atttgtaaat taaagagagg gtgtctcaaa atgaactttt
     6181 tagttcatga ggcactcttt ttgtacaaaa tcaaatatgt tttaatcatt tcaacaatga
     6241 aaatttcatt cctgtagaaa gtctaacttt taggtcttag tacaatctct attttgatgt
     6301 agccactttt ttagatggtt taataattta tcagttatta ttgtaatatt tgatactaag
     6361 taacataact ttgtctaact atgataaaat agttatacaa taaaggtctt gtaaggagtg
     6421 aataatgtgt ggctaataaa atcagatttg tcaaatctaa cattaaataa tagaaaaata
     6481 aattgtgtaa ttaatggtat tttaatatga aaaaaagaga aaaagtattt gatgggctag
     6541 tttttaagca tatattaatc ttattaatac tattagtttt gattgattta acaagtacca
     6601 tcatatatgc aagtaactct atggaaatta cttctagaaa tatgattgag gcatcaaaaa
     6661 gagaacttga aaactattta gaagtaaaca tatctttatt aaaagctctt agtcaagatg
     6721 atagattttc taatggtgaa acatcactaa ttgaaaaggg aaaattatta agtccatatc
     6781 aaaaggaata taatcttttt atgattggta taacagatac aaaaggaaat gcttcaagta
     6841 catataggga aaatgttaac tcaataaaag ataagcctag ttttgagaaa gcaattaaaa
     6901 caaagcaagt agtagtttca gatattgaag tcagtaatgt aacaggtgac aaagtattta
     6961 tcatatatgt accaattgta aaaaataatg agatgattgg gaccattttt gcttcttttt
     7021 attttcaaga tgtaaataat ttaataagta gaagcaattt tgatgattct attaaatttc
     7081 taatgataga taaagattat actattattt ctcatccaaa caaaaaatat gtaaatgata
     7141 aaagtaagat gcttgattta gaagggaata ttataggaac cactaaaagt gaaatcttaa
     7201 aaaatataaa taaaaaatgt caaggtgatt ttttatcttg ggataattgg agattatata
     7261 atgtaaaata tacgaacata aagtggacta attggacttt agtatcaaaa tgtaatatat
     7321 ttaagaactt taaaaatcta atagttaatt ttatgataaa gttgtatttt tatatagtaa
     7381 tatttatgat attgtggaaa ttaagtaatg ctaaattaat agaacaactt aaaaaattgg
     7441 cttattatga ttctttaagt ggaataaaaa ataaagaaaa gtttagaaaa gattctatgt
     7501 atattttaaa gaattattat caagataatt tttacttagt acaattggat gtcaataaat
     7561 ttaaatatat aaatgagatg tttggatatg ctgaagggaa taagatatta attcatatat
     7621 cacaggtttt aaataataat acaaataaat atgaaatttg tgctaggatg gataatgacc
     7681 attttatatt gttgattgct tgtaacactg aagatgaact tttaaataga ttatctaaaa
     7741 taaataaaga aatttgtaac ttaaatacta ctaactcatc taagtataaa attgttatgt
     7801 caagtggaat atataagatt actaaaaagg atgatattaa aaagattgac ttgttaattg
     7861 atagagcaaa tattgcagca aaaagtaaaa aggaaaaata tgaacattca tattcatttt
     7921 ttaatgaaga tacaagaaat cgattgtaca aggaaaaacg attggaagat aatatgaata
     7981 aagctttaga aaaaggtgaa tttatagtat attatcaacc taagtatagt ttagatgatg
     8041 taaatgaaat tgaaggagca gaagccttga taagatggaa tagtccagaa tttgggttta
     8101 tttcacctat agattttgta cctttatttg aaaagaatgg ttttattgta aatattgata
     8161 tgttcgtgtt tgaagaagta tgcaaaacat taaacaagtg gataaataaa gggtatactc
     8221 ctgtaccaat ctctgtaaat atgtctagag ttcatttata tagggataat tttatagaaa
     8281 atataactga tttgataagt aaatacaata tatctcctga gtttattgag cttgaactaa
     8341 cagaaagtgt tgtgtttgat aatttaaata tattgattga tataatgaaa aagattaaag
     8401 agataggatt ccttatatca atggatgatt ttggttcggg atattcatct ttaaatctgt
     8461 taaaagacct ttcttttgat attttaaaat tagatagagg atttttaatc gaaacaacag
     8521 atacaaaacg tggaaaaatt attatttcta agattgtgga aatggcaaaa gcgattgata
     8581 ttaaagtaat ctgtgagggc gtagaaactt atgaacaagt agagttcttg aaagagatag
     8641 gttgtgataa ggttcaagga tacttatttg ctaaacctat ggtattagat gaatttgaaa
     8701 aacacttaaa ctttaaattt gattaatcat taataattat tagactataa gataccctaa
     8761 caacttactt acaaatatgt aagttaattg ttagggtatt tttatggtta aagtagtata
     8821 ttcacgatat tatggtattg tagtaaaaat aaaaaattat ggagaatata aaatgaacat
     8881 attgagtatt agaaatatat caaaaacata ttgtggaaat attccattta aagcattaga
     8941 taaggttagt ctaaatatag aaaagggaga atttgtgtct gttatgggac catctggaag
     9001 tggtaaatct actcttttaa atataatatc tacagttgat agacagagtg aaggtgaggt
     9061 tgtattagat ggatatgatg taagtaaatt gaaaggagaa aaacttgctg aatttagaag
     9121 gaaacaactt ggttttgtat ttcaggattt taatttgatt gacactttaa ctgttggcga
     9181 aaatattatg ttgccactta cactagaggg ggcaagtatc aaagatatga atatacaaac
     9241 taaaagtata tctaaatttt taggaataga taaaatttta gatagaaaaa cctatgaaat
     9301 ttcaggaggt caagctcaaa ggtgtgctat tgctagagct attataaata aacctgccat
     9361 acttcttgca gatgagccaa ctggaaatct tgattcaaaa tctactgatg atgttttgaa
     9421 attatttact aggataaaca aagaacaaaa ggtaacaaca cttatggtaa ctcatgaagc
     9481 atatagtgca agttattcag atagagtaat ttttataaaa gatggttgta tatatacaga
     9541 aataaaaaaa tctgaaagca gtaattcttt ctattctgat atactagctg ttttgtctca
     9601 aataggaggt gtaagatagt ggattttaga aagtttgctt ataataatat agttagaaat
     9661 ttcagggcat atttagcata ttttttaagt tctgtttttt ctataatgat attttttgtt
     9721 tttgcagtgt ctatgttcca tccaattata acaaaatctg gaatgcagag tggttctact
     9781 gcatttatgg ctcttatgac ttcagaatta ataatattcg gttttagtct actttttata
     9841 ttgtactctt taggaaattt cttaaaatat agattgaaag attttggagt attgatgata
     9901 attggaatga gtaacagaca actgaaaaaa ttgatattaa ttgaaaattt gataatagga
     9961 tttttagcag tgatgttagg aatattactt ggtttatcaa tttcaaaatt gtttttattg
    10021 tatttaagca aactgtttta tatgaattta actgaatttt attttccagt aaaagcaatg
    10081 ctattgacta ttgtatcatt tatgatttta tttttgataa caggaccaat gagcttaaaa
    10141 ttattgagta aaaagagcat tttagagctt ttagtaggta caaaaaagcc taaaaaagag
    10201 ataaaatctt caaagctatt tggaataatt ggtgtaatat gtcttatatt aggatatgct
    10261 atgatactat ttggcaataa gttaaaaata aatggaggta tgtatacaac agcactatta
    10321 attactttgg ggatattttt atttttttct caatttacta ttttgatatt aaagtattta
    10381 aaaaataaag aaaaatttta taagaacaaa gtaaatatgt tacttataag taatttgata
    10441 tataaattaa aggataatac acgattgtta tttttaataa caatactttt atcggctact
    10501 ttagtagcat ttacaacaac ttcaacactt gtaagttctc aaggagaagc ttctaaaaat
    10561 aatttcccaa tggtttactc atatttttca gagcataata atgaaaaaga atatgaagaa
    10621 ctacaacaaa ttagagaagc aatcaaagga tatgattata aggagttaag ttttcctgta
    10681 cttaaatata atagggaagt cttactaagt gtaaagaatt ataacaaatt atcaaaggaa
    10741 ttgggattag ataaaattag ttcaaagtta gaaaaatatg aagggattat aattcctagt
    10801 agcaattcgc aagaacatat aaattcttta aaacaattaa aaaattatag tataaaaaat
    10861 ttaaatatag aagtaaaaaa atcaaaaaat aaaataattt ttcctacagg actgtttagt
    10921 aaagttgtgg ttataagtga tgacttatac aatgaagtaa aaggtgaatt ttcaaaaatt
    10981 aattttcatg gatttgatta taaaaactgg caaagcagtg caaagataac tgaaaattta
    11041 aaagaaaaac attttaattt ggatagagaa tataacaggt catatttttt aacattacca
    11101 gatatgtatt tagtagagtt acaacaaagt aaaattcttc aatttatggg tgtttttata
    11161 ggagtgatat tatttatagc agtttttagt tttttatact tcagattata tacagacgag
    11221 atagaagata atgtaaagta caaaaaacta tcaaaaatag gtctatcttt taaaaatatg
    11281 aataagattg ttagtataga gataggtaca ttgtttttta taccgtattt tatagcaatt
    11341 ataaatagca taatttcttt actgttttta aatcaagtat taaataatag ttttaagtta
    11401 gaaaatttgg gtatattact actgatatca tctatatatt cactttattt ttacttatta
    11461 aaaaaaaatt atactagaaa agtttggttt tttgattaaa gtggtatata atagatatat
    11521 aaattaatat ttaaaaagta gttaatatta ggggagggca tttgcacctc ccttgtaata
    11581 tgtggaaaat tgaatattag aagaaggagg agacttgatg gaaaatcaac aattattagg
    11641 aacagaacga attagtaaat tactactaaa atattctata ccagctataa taggtatgtt
    11701 ggtaaatagt ttatataatg tagtagatag aatttttatt ggaaatatac caggggttgg
    11761 accacttgca ataacaggtc ttggtgtaac aatgccaata atgacaataa tacttgcatt
    11821 tgggatgttg ataggtatag gaactactac taccatatca ataaaacttg ggcaaggtaa
    11881 agtagaagag gcaagaaaac ttataggaaa tgcaatgacg ttatctgtaa taacaggaat
    11941 tatcataatg atacttggaa tattatttgc aaataaaata cttacattgt ttggagcaag
    12001 tgaaaatact ttaatatatg caaaatcata tatcaatatt atattattag gtacagtagt
    12061 aaatcttctt tccttttctt taaaccactc aataagagct gatggtagtc ctaaaatttc
    12121 agcaggaata atgatagtag gatgtttaac taatatagtt ttagactgga tattaatatt
    12181 tggattcaat ttgggaatac aaggagctgc aattgctaca gtaacttctc aggctcttac
    12241 agctatttta actatagggt attatataag tggaaaatca aacttacgat tcagtaaatc
    12301 taatctaaaa ttagataaaa agctaataaa agcagtgttt gcaataggta tgtcgccatt
    12361 tgctatgcaa cttgcagcaa gtttagttca agtaatatca aatattgcac ttaaaactca
    12421 tggtggagat ttagctatag gggctatggc tactatttct tctattgcaa tggtattttt
    12481 gatgcctatc tttggaataa atcaaggagc gcaacctatt ataggtttca attatggagc
    12541 tgaaaaatat gatagggtta aaaaagcata tttaggttca ttagtagtag ctactataat
    12601 tttatgtatg ggtatggtag tagttatgct tttcccagaa gctattatag gtatatttaa
    12661 taaagaccct gaacttatga atatatctgt taatggatta agaatatact tacttatgtt
    12721 gccaattgta ggcttatcag ttacaggaac aaactttata caatctatag gaaaagcaaa
    12781 aatggctatg ttattgagcc ttttaagaca agtaatttta ttgataccag cagtattaat
    12841 tcttcctaca ttcttaggtt tacagggtgt atggacagct caaccagtgt ctgattttat
    12901 agctacagta attacaggta tagttgtatt tagagaatta aaaagatata ctcctaaaac
    12961 tgaaaaatta aatgaaaatg aaagactaaa cgagataact actgagtaaa aatataaatt
    13021 cagataattg agcaagaaat tgcaaaaaat acgattatat gataaaataa aaccgctgtc
    13081 aaggataaga gaaattctaa ttatggagtg attttatgga atttacaaag aaaagcaaat
    13141 taaaaaatat gtatgtaaac atgaatgtag ctagaatgtt gtctaagata aatgagtata
    13201 agggtagaca actgctttac aaaaaacaac caaaagaaat tttagaaaac ttagaaaaaa
    13261 aatctttagt agattgttct gaatctacat acattggaaa tcaaagagat agtagtaatt
    13321 ttaatctaga aaagttgata agtaatgaag ttacacctag aagtagagag gaattgagta
    13381 ttgttgaata cagagatgtg gtaaaaacta ttaacagtgc ttatgagagt attcccatca
    13441 gttcacaaac aattcttgag cttcatggat acttatataa attttcatct actagaggag
    13501 gaagttataa gtcagataat gattttattg aacacaattt aaaacctagc gaatttataa
    13561 gtactgattc aagtgtcaat aaagttgaaa aagctgtaga agaaatatgt gaagcttata
    13621 atacattgat agaggaagat gaaattgata tattaatatt aatttctgca tttgtgttag
    13681 atttcatatt gatacatcca ttcaaagaag gtaatataaa aatggcgaga gtacttatcc
    13741 tattgttact aaataaaaat gggtatgaag ttggaagata tataagttta ggaaagatat
    13801 ttgatgatag ttcatatgaa tattatagta atttaaatta tttgaaagcc tcaataggta
    13861 gtgaaaaagc agatatgaat gcatggattg agtatttttt agagacgata ttaactgcat
    13921 atgagaagct ggatgatagt ttaaatatat ctgataagaa aagacaaaca aaaactagta
    13981 gaatagagaa aataataaat tctacccttg gatattttac aaaagaagat ataagagatt
    14041 tatgtcctga cattccagaa cctactataa atagagtttt taataatcta agaaaacaag
    14101 ataagataga agttgttgca agaggaagaa gtgcaaaatg gaagaaaaaa tattaaaaaa
    14161 tgtttagata actaaaatgg tatagtgttt tacactatac catttttaat gtcattagca
    14221 tgagcaataa tttaacaact taagcaatat caattttatt tattgattat ttttgaactg
    14281 aaaaatggcg ttatatggta tacctcagtg gtaattaatt ttggatgaga agatataata
    14341 ttacagaata tattaggagt atatgtagaa aaaatataat aaaagtgtga ggcttagtaa
    14401 ttaacaaatt atgtggcttc acactttttt ataaaataaa gatacttagc agaataatat
    14461 tttagttgat atgtttataa gatggtactt gtttgggtaa aagtataaat agtaaaaaag
    14521 tagaagtaat gtgtataaaa acataagtta cattctgtaa taatagttat tttataattc
    14581 aacaaaagaa tagaatgggt atatttgtga aataataata taaaataatc ttaggagtga
    14641 gcttatgaag aaaaaattat tatatataaa tgtaaattca aaacctgaag acttatcttc
    14701 aagtaaaact gtagcaagaa aatttataaa taaatttatg gaaaaaaata aagattttga
    14761 ggttgaagag atagaccttt ataaagaaca tataccaaga cttgagtatc agtactttga
    14821 aaaaagaaat agcatagtta gtgaagaaaa tgctaaaaat ttagacgata aggatagaaa
    14881 agagcttaca aagattagaa atctttgtga ccaatttgtg aatgcaagtg tttatgtaat
    14941 agcagcacct atgtggagtt tatcttttcc agcaccactt aaagagtata tagattgtat
    15001 aattcaagat ggaaaaacta tatcttttga aggcaatgat aaaccacaag gaattttaaa
    15061 tgatattgat agaagtatgg tatatataca atcttctggt ggacacatac catgggtgtt
    15121 gaagccagtt atgaataaag ggttaaatta tgtggaaagt attatgaaat ttataggaat
    15181 taaaaaattt gatgaattgt tagtagatgg aactggaact agtgaagaag aaagacaagc
    15241 tgcaatagaa aaagcatctg aaaaaataga tggtataata gatgggatga aattttaatt
    15301 ataatatttt attaaaaata taaaggaaaa acctctttta atgtagaaac ttatattgaa
    15361 ggaggcgaga atcatgcgag aagaaaaaag taatgaaaag tatgattgtt attggtgtaa
    15421 tcaagagaat aatttctgtg tagaaataaa agataatata gtcatgatag atgatggcac
    15481 tggtacgtta aaacaagccg ttttcatagg gtataaacaa atccaaatta atttaaactg
    15541 ttcacattgt caaaacttaa atagaataaa attaaatttg tagaatttta aaatggaaat
    15601 tattgaagga attaaaaata gaaagtgtta atatttagac tgaaaattat attttataca
    15661 ataaaagttt atttaaataa cattgtattt taatatttat tttcagtctt actttattaa
    15721 aattattttt tggttatgtc tggaaaatat ctgtctacta atttttctga aatatttttt
    15781 aattggtcag ccattgaagt cagacttaat aaggctatca ataaatcatc tttattttct
    15841 ggatttatac taatattttc aaaattagac tctataaagt ccggtatatt ctttctttga
    15901 ttttctttta ttaacatgaa tttttcgtaa catgaaacta aatctttttc actgaaatta
    15961 tccttttcat acacaccatg taagattctt ttaatttctt gtattgtaag tgtatttttc
    16021 atagaataaa taattatcat ctgtaaaatt tgttctttag tatacttttt tcccttaact
    16081 ggtttgatta atccctcttt gctgtaatta tttatcatgg ttttagtaag aattttatca
    16141 ctttcaaatc ttttattagc agaaagctta ttgtcaatta gagaaataat ttgatccata
    16201 tataagtcaa tagatggaat atcgtttgaa gttatatctg cattatttag tgtttctagt
    16261 attatagtat taagttcatt attcatcata tgttacctcc ataatatata taatacacta
    16321 aattagaaag taaaacaata ttaaggcttg taaaaaaaac ataaagtggt ataattaaat
    16381 atataatata gtaatcaaaa ctatatttat gaatctgatt tttaaaatac ataattgtag
    16441 gaggacgaaa tgtatcaatt ttttttaaag ggtagagacc caataagcag tttaactcat
    16501 tttataggtg cttgtttatc tcttttagca actataattt tagtttttca atctgttaca
    16561 ttacaggaaa cttctctctt gatgatagta tctgtatctg tatttggact ttcactaatt
    16621 gctttatata gtgcaagttc gtattatcat tttttaaagg ggacaccaga acaagaattg
    16681 ttttttagaa aagtagacca tgcaatgatt tacgtattga ttgctggctc ttatacacca
    16741 atatgtctga attttatgga aaaaaaagaa ggcataatat ttgttacagc aatatggata
    16801 gttgcattta ttggcataat aataaaaata ttttggatgg atgcacctag atggttatct
    16861 accagtatat atttacttat ggggtgggca attgtttttg atataaatgc attcaattca
    16921 attcctaagg attgccttag actattaatt atggaaggtg tatcttactc tataggtgcg
    16981 attatttata taattaaaaa gcctaatata agtcctgagt ttggatttca tgaaatattt
    17041 catattttta ttatgatagg ttctttattt cactttttag cagttttatt gtatgtttta
    17101 tagttttaaa agatttgatt aattgatact aaaattctac aatgaataaa atggaactgc
    17161 cttatataga ttatatctta gacaataaga tagttcctta ttataaatat taaattctac
    17221 ttataataaa attttgataa aaatagtcca ttcaaaatgc ctattccata attcctttaa
    17281 tgtacttttc atttacgaca tatagtttga atttatgtac tataattata tcctctaaat
    17341 tctctgaaac actaacaatt ctgacacatt tgtttctaat ttctacaggt aaatctaaat
    17401 catttgttaa attcacatag tcatcatatt tatacataaa atcatccctt tacattaaga
    17461 tatacttatt atactatatt ttaataagtt tttttaaatt attacaattt t
//
LOCUS       DAEPIN010000076        17087 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.076, whole genome shotgun sequence.
ACCESSION   DAEPIN010000076 DAEPIN010000000
VERSION     DAEPIN010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17087
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.076"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            39..416
                     /locus_tag="KR250_003039"
     CDS             39..416
                     /locus_tag="KR250_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBH0068934.1"
                     /translation="MKEEILIEKLLRAELIVMEYLWKKDTLMPKKEIVKEIKQIYGWH
                     KSTIKILLKRLIDKGYLARDIIKFQSYYKIIIDNEEYYAFKKKVLKSSKSRKIMRSLT
                     TTHKNVSKEKLDSLEEYYKNLKE"
     gene            521..904
                     /locus_tag="KR250_003040"
     CDS             521..904
                     /locus_tag="KR250_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBH0068935.1"
                     /translation="MKISKLPEAELKVMRYIWEANKVLTSRELVAAMEEKYEWNESTT
                     FTVLKRLERREFLSTEKIGKLTHYNILVKEKKYLRFETKEFLKNIHKNSISSLISALH
                     SKDDEVDEDKLIELEEHFKNLKEKE"
     gene            1089..1673
                     /locus_tag="KR250_003041"
                     /pseudo
     CDS             1089..1673
                     /locus_tag="KR250_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896033.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            1699..2112
                     /locus_tag="KR250_003042"
     CDS             1699..2112
                     /locus_tag="KR250_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBH0068936.1"
                     /translation="MKKIILASHGDFSKGLLNAVKMIVGDLADCVSSYGLYPGQSASD
                     FALELEKTISEDKECEYIILSDLYGASVCTAMLRLTNLHNVRLFSGMNLNMVLELLTR
                     FSDELTVEDIDQLVEESRRGIQSVTLQIKEEEEVF"
     gene            2116..2859
                     /locus_tag="KR250_003043"
     CDS             2116..2859
                     /locus_tag="KR250_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane biogenesis protein"
                     /protein_id="HBH0068937.1"
                     /translation="MKKKGILSVFKSKKPIIAMIHLKGDTPEDIFERAKKEITIFEEN
                     GVDGIMLENYYGNYYDLERILEYVSKANLSIPYGVNCLNVDTMGFELATKYNASYIQV
                     DSVVGHVKPRDEATLEEFFKLQRSKCPAYLIGGVRFKYQPVLSENDVEEDLKIGMTRC
                     DAIAVTENATGQETSMEKIELFRKNLGDFPLVIAAGVTLENAKKQLKLGDMAIIGSYF
                     KDNYKDFGDVSVEHVKTFMDEIKKIREEL"
     gene            2859..3329
                     /locus_tag="KR250_003044"
     CDS             2859..3329
                     /locus_tag="KR250_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBH0068938.1"
                     /translation="MIKLVRVDHRLIHGQVAFTWTKFLSTDCILIASDDLLKDELRMA
                     GLKMAKPSNVKLVMKSIADSIKALNSGVTDKYNLLILCESVEDVYRLAKEVKAIKSIN
                     LGGTKSDDNRENISKAVHVSKDDIKMIKELDSEGVNVFVQLVPDDDATNVMKLI"
     gene            3346..4104
                     /locus_tag="KR250_003045"
     CDS             3346..4104
                     /locus_tag="KR250_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBH0068939.1"
                     /translation="MEFTQVILITLIAFFAYMHSFVGSTMHNRPIVVAPLVGLALGNL
                     HTGIVIGSTLELVFMGAFPVGASNPPDFVSGSIIATAFVILTGQDVSAAVVLAVPIAT
                     LVLLIDNFLMTVVLTWGAHIADRYAEEGNIEGVERVQLLFGIGNKLILAIIVGIGFSL
                     GVPVIEKILSFIPSYVTHGMDVAAGVIPAIGFAMLARMMLNKKTVAFLLLGFILVAYL
                     NITVTGVALFGLAIALIYVNFVGEKEVIVDDNEF"
     gene            4088..4930
                     /locus_tag="KR250_003046"
     CDS             4088..4930
                     /locus_tag="KR250_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBH0068940.1"
                     /translation="MTTSSKKLETISPDSKITRKDFWKCFRRSLTLDSSWNYERMQNI
                     AYAYMMAPIIRRLYKDDKEKKSKALKRHLEFMSVTPHISTLLVGISGAMEEENAKNKE
                     FDANSINAVKSSLMGPVSGIGDSFFWGTLKLIAAGVGIALASQGNIMGPILFLLIINV
                     PHFIIRYICLDKGFKYGTQFFKDVSGSSIVSKVMEAASMLGLMVIGGMTASNVMLKLS
                     VNVGSGEWAEPIQTYLDQIMPCMLPAMIFGIMYWLLGKKVKTTTILISVMIICIVLAA
                     IGVV"
     gene            complement(5090..5995)
                     /locus_tag="KR250_003047"
     CDS             complement(5090..5995)
                     /locus_tag="KR250_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBH0068941.1"
                     /translation="MDFKQLEVFVAVAKHQSFSKAARELFLTQPTVSAHIQNLERELE
                     TVLINRSNKVITLTKSGEILYEHAIYILNNCKRAIYDIKEYSGKIEGIIDIACSSIPE
                     TYILPDFMKSFSMSYPDVKFSISHYDSQYAISEILNERISFGLVGSKINNPQIEYLDL
                     LDDELVLITPSDFKIDNKNNCIDIGELAYLNFIMRKEGSGTRNLILNTLSKNNFPVSK
                     LNVIAHVESNEAIKEMVRLGLGVSFISYISAIDYLNAGKIKCYKIKDVDFTRKFFFIY
                     SKKKTFSPLEDKFLNRLCEYFEIII"
     gene            6357..7091
                     /locus_tag="KR250_003048"
     CDS             6357..7091
                     /locus_tag="KR250_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0068942.1"
                     /translation="MKLKRSLVCVTILGIILVGCHKENTKEKNQVASKATQQKTMTKV
                     QNDVNEIMNKDYKYIIKNMGIPYNTFYYIKPKVLKESNTMQDINTSSYMTLVYPKYTG
                     NDELDGSALYVDINENKVVNVETNSFSSQGISVIDAESSIVIEKSDHEKSAVSLENFR
                     HIDLGEYVGVEDSRINEIVGDANYDLTAYNHEGSKVVKSYRLKEDNKILKKEVLTISI
                     VDNKIKSIKTIESDKIVKIIKGTLLE"
     gene            7302..8483
                     /locus_tag="KR250_003049"
     CDS             7302..8483
                     /locus_tag="KR250_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HBH0068943.1"
                     /translation="MIELEDAIKIIEKNVLPINRVKIVNLIDAVNKVVAEDIYSTIDS
                     PPFDRSPYDGYAYNANSENRNLKVIGTLYAGDVFNRVLNKNEAVKIMTGGKIPQGANC
                     VIKKEDVTVIGDMITLNIKLKEYDNYIFKGEDIKKGQLIINKNQNIGYDGIGVLASLG
                     ISKVTVYDDLKVGILNTGTELQDINETLENGKIYDSNRYTLYSRLLKLGIQADTICNC
                     SDDILELEKKVKNMLQSVDFLITTGGVSVGDKDLIPDVFKKIGATELFWKINIKPGGA
                     CYVGTLGEKLLFGLSGNPHAAIVVFDNVIVPVIEYLTYKKGTHHIKAIFKGEFNKFSN
                     KDRFVSGKLYTSEGKLYVELNDKKDAKARLSATLRSNCMIKIKKNETIKENQLVDVVI
                     R"
     gene            8467..9039
                     /gene="mobB"
                     /locus_tag="KR250_003050"
     CDS             8467..9039
                     /gene="mobB"
                     /locus_tag="KR250_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HBH0068944.1"
                     /translation="MLLLDSFMKGHNFMYNILSVVSYSGVGKTTLIEGIIKELNKKGY
                     RVGVIKHTCHDFDMDEEGKDTYKHRKSGARKVCIISDKRVAYIEELKEKNPLYKMIQL
                     YEDMDLIIIEGYKNYRFKRLEVTRKGKYEDILSNKSDLIGIVSDIEYKLNIEQFNLND
                     YKNISKYIESCIKNEILSISNVEIKNILKE"
     gene            9158..9433
                     /locus_tag="KR250_003051"
     CDS             9158..9433
                     /locus_tag="KR250_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBH0068945.1"
                     /translation="MKKILVACGAGIATSTIVCDRVERLVKENNIQAEVEQCKIAECS
                     TKQEGADLIVSTTILPTTYDIPTIKATGYITGINTTALDKKILNALK"
     gene            9688..11388
                     /locus_tag="KR250_003052"
     CDS             9688..11388
                     /locus_tag="KR250_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0068946.1"
                     /translation="MIKQFVKYYKPYKKIFTLDLIAAFLFSLCDLVYPMITRNIMDDV
                     VPNKNLRMLVVFAVALILIFIAKAGLNYFMQYWGHVIGVDMQADMRNEVFTHLQRLPN
                     TYFDNNKSGVTMSRIVNDLMDITELAHHGPEDLFISIVMLVGSFFILIDINIPLTLII
                     FAILPFIIWFAIAKKDKMNIAFMKSRVTIGDVNATLENSIAGMKVTKSFCTEKEELNK
                     FVRSNKLFRRARQDSYKVMAEYYSGMNLYMDILEWVVVIAGGYFTYIGKITLGDFAAY
                     ILYVKMFIQPMKKLINFTEQYQNGMTGFKRFIEIMEQDHQKEAKNPIELENVKGDIEI
                     ENISFTYEDKTQVLDNLSLSIKAGKTIALVGPSGGGKTTLCNLLPRFYEFDKGDIKID
                     GKSIKDVSLKSLRKNIGIVQQDVFLFTGTIRDNILCGNPNATDEEMIAAAKKARIHDF
                     VETLPDGYDTYIGERGVKLSGGQKQRISISRIFLKNPPIIILDEATSALDNVTEREIQ
                     ESLEELSKDRTNLVVAHRLTTIKNADEIIVLTDKGIEERGTHEELVNKNGVYSRLHNN
                     "
     gene            11751..12938
                     /locus_tag="KR250_003053"
     CDS             11751..12938
                     /locus_tag="KR250_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0068947.1"
                     /translation="MEAITAFCLVLVSLTIGDIVSAKTKAFVPSVFVSAIIFIVGFWT
                     FFPENIVDLAALGTPLAQLGMLLLITHMGTMMSIKELAGQWKTIVIALAGIVGICVGA
                     LALGTVVFGWDTAVIATPPLTGGLVASIMMADAATAKGLASLSVLAILMYVAQGFAGY
                     PITALMLKKEGKRLLSDFRSGKVTVNTDEEKVKDLPEQKSRFKIIPDLPEKYDTTYMI
                     ILRLGFVAWLAVQFTNLTNEVVSRYVICLIFGVIASELGIVDRKPLNKSGSFGWLMTS
                     LMAFIFAGLAKATPAMLVEMAVPLVGIIIFGVIGMGIFSIIVGKLLGESKEMAFSVSL
                     TSLYGFPPNYILTEEAVKALAETPEEKEFLMDKMLPKMLVGGFTTVTIASVVIAGIFV
                     NLI"
     gene            12998..14161
                     /locus_tag="KR250_003054"
     CDS             12998..14161
                     /locus_tag="KR250_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBH0068948.1"
                     /translation="MDIKEITKSYKDYVIKLRREFHENPEKSMEEVRTSKRVKEELDK
                     IGIPYVSAGGTGVIATIKGANPGKTVALRGDMDALQVVECTDVEYKSKNEGLMHACGH
                     DGHTSMLLGAAKVLNDIKDSINGTVKLFFQPGEEVGKGARAMIQDGAMEGVDSVFGIH
                     LWTDVESGTISVEEGPRMASADFFKITVKGRGGHGSLPHQGVDAVLASSAIVMNLQSM
                     VSREVSPLEPLVVSVGVLNSGTRFNVIASEAVLEGTIRLFNPELRKQIPGILERIAKS
                     TAEAYRADAELEYGYLTPAVINDKECSKIATDAAIKLFGEDCITLFEKVTGAEDLAEF
                     MNIAPGALAFVGARNESKGACYPHHHGCFNIDEDALEIGTALYVQYAVDFLNK"
     gene            14312..15115
                     /gene="zupT"
                     /locus_tag="KR250_003055"
     CDS             14312..15115
                     /gene="zupT"
                     /locus_tag="KR250_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HBH0068949.1"
                     /translation="MNNVIFAFLITLLAGLSTGIGSCIAFFAKKTNTKFLSISLGFSA
                     GVMIYVSMIEIFPKAQDALTKSMGEKLGSWSTVIAFFIGMAIIAVIDKLIPQEENPHE
                     IKKMENIEEKNIKKNKSLLRTGIFTAMAIAIHNFPEGLATFISALDDVTIAIPIAIAI
                     AIHNIPEGISVSVPVYYATGDKKKAFYYSFLSGMSEPLGAIIGYVLLRNFLNDITLGI
                     VFAIVGGIMVFISLDELLPSAREYGEHHLSIYGLIAGMGVMAISLLLFK"
     gene            15211..15771
                     /locus_tag="KR250_003056"
     CDS             15211..15771
                     /locus_tag="KR250_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HBH0068950.1"
                     /translation="MSAMEKKIKFKFDWYSSPDKIEKWLEDIEAKGYNLYKINKFGGI
                     FYFHKGSPRKIKYTVDYYYDVENNYFDEHKSNGWNLVFRSIGSFMGTSAWYIWSKEYK
                     DIPPKLYSDSDKKNKVKSILFMNMFIFFMLVVIFIYLLNIEIRNLYSGYGASVTAIVL
                     QIILLILYAVQLYRRLMYYLRLKKFK"
     gene            16021..16734
                     /gene="rgbR"
                     /locus_tag="KR250_003057"
     CDS             16021..16734
                     /gene="rgbR"
                     /locus_tag="KR250_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RgbR"
                     /protein_id="HBH0068951.1"
                     /translation="MIKIAVCEDEKETQLLIEDYLENILKDISIEYEIQKYISGEELL
                     ESNLKDIDILLLDIKMEKLNGMDTARKIREVDNEMEIIFVTSLIDYVQEGYEVRAYRY
                     LLKPIELEELKKHVLTCIKDIEINKESHITIKNKSNTYKIYLNEIKYIEVQKKDMLIH
                     TINKNFDIKYSLGKIEKELNPYKFIRCHKSFIVNLRYVENIKPNTAILESGEEVPISR
                     YRYKEVKEKFLKFLGDTIC"
BASE COUNT         6706 a         1731 c         3033 g         5617 t
ORIGIN      
        1 ttaatttaag ttagacgaat ttaaggaagt gacataaatt gaaagaagaa atattaatag
       61 aaaaattatt aagagcagaa ttaatagtaa tggagtattt atggaaaaag gacactctaa
      121 tgcctaaaaa agaaattgta aaagaaataa aacagattta tggatggcat aaaagtacaa
      181 taaaaatact actaaaaagg ctaattgaca aaggttattt ggctagggat attataaaat
      241 ttcaatctta ttataaaata ataatagata atgaagaata ttatgctttt aagaaaaagg
      301 tactaaaatc tagtaaaagt agaaaaataa tgcgttcact taccactacg cataaaaatg
      361 taagtaaaga gaagttagac tcattagaag agtactataa aaacttaaaa gaatgatatg
      421 gtaaaaattg gatggaatta tttaccttaa ttctcttgtt tgtaaaactt tattgatata
      481 gaatttaaca tatacataaa tcggtaaagg ggtgtagact atgaaaatta gtaaattacc
      541 agaagcggaa ttaaaagtta tgagatatat ttgggaagct aacaaggtat taacttctag
      601 ggaattagtt gctgcaatgg aagaaaaata tgaatggaat gaatcaacga catttacagt
      661 tttaaaaagg ttagagcgaa gagaattttt aagcacagag aaaataggca aacttacaca
      721 ttacaatata ctagtaaaag agaaaaaata tctaaggttt gaaacgaaag aatttttgaa
      781 gaacatacat aagaattcaa tttcaagttt aatatcagcg ttacatagta aagatgatga
      841 agtggatgaa gataaattaa ttgaactcga agaacatttt aaaaatttaa aagagaaaga
      901 ataaataaag attattttag gtggcaaatt atttttgcca ccttttttgc caccatgaca
      961 gtttaataaa ataaaataca aataaagaaa tataaataaa atagaataaa gaaattgttt
     1021 tagataggtt gtaaataaat gtactcataa aaacataaat aaatataaat aaacataaat
     1081 aaaatataag aatttctgtg agaaatgcac ttaatgaact tgtaaaagaa gatatattat
     1141 gtaaaaaacg tggtaaaggg acttatgtaa caataccaga gagaatagaa gcaacatgtg
     1201 caggaaatag ttttactaat tcttgtcatc gtattaatgc aaaaccaagt accaaaataa
     1261 tctctgtttt aataaaaaaa gcagataagc aagtagctga agctttaagt attgaactag
     1321 aagagaaggt aatatgtatt aaaagactta gattaataga tgaagtacct gttatttttg
     1381 aagttgacta ttttagaata gattacatgt ttttattaaa agaagaatta gaagataaat
     1441 ctttaatgga ggttatatct aataatataa gcacattacc taaacgagta gaaaatatat
     1501 ttgaagtaaa acattcaaac aaagaatatt ctgaccatct taaatgtgca agtaatatgc
     1561 cactattgaa agtaagacaa tcggtgtata cagaaaataa tgatgtctta tattacaatg
     1621 agcaatttat aagaagtgat aagtacaaat atgcagtatc agcagaaata taatctataa
     1681 tttagtagga ggatgagtat gaaaaagata atattagcat ctcatggaga cttttcaaaa
     1741 gggcttttaa atgcagtaaa aatgatagta ggagatttag cagattgtgt cagttcgtat
     1801 ggtttgtacc ctggacaaag tgcttcagat tttgctttag aattagaaaa aacaatatca
     1861 gaagataaag aatgtgaata tataatttta tctgacttat atggagcaag tgtatgtaca
     1921 gcaatgttaa gattaactaa tctacataat gttaggttat tttctggaat gaatttgaat
     1981 atggtattag aacttcttac aaggttttct gatgaattaa ccgttgaaga tatagaccaa
     2041 ttagtggaag aatctagaag aggaatacaa agcgtgacat tacaaattaa agaagaagaa
     2101 gaggtatttt agtttatgaa aaaaaaggga atattatcag tatttaaaag taaaaaacca
     2161 attatagcaa tgatacactt aaaaggagat actccagaag atatctttga aagagctaaa
     2221 aaagagatta caatctttga agaaaatggt gtagatggaa taatgttaga aaattactat
     2281 ggtaattatt atgatttaga aagaatactt gaatatgtat caaaagctaa tttatcaata
     2341 ccatatggag taaactgttt aaatgttgat actatgggat ttgaactggc aacaaaatat
     2401 aatgctagtt atatacaggt tgattctgta gtaggtcatg ttaaacctag agatgaagct
     2461 actttagaag agttttttaa attacaacgt tcaaaatgcc cagcgtattt aattggaggc
     2521 gtacgtttta aatatcagcc agtattatca gaaaatgatg ttgaagaaga tttaaaaata
     2581 ggaatgacaa gatgtgatgc tatagcagtt acagaaaatg ctacaggtca agaaacatca
     2641 atggaaaaaa tagaactgtt tcgtaaaaat ttaggagact tcccattggt tatagctgct
     2701 ggagttacat tagaaaatgc aaaaaaacag cttaaattag gagatatggc aataatagga
     2761 agttacttta aagataatta caaagatttt ggggatgtta gtgtagaaca cgtaaaaaca
     2821 ttcatggatg aaatcaaaaa aattagggag gaattataat gattaaatta gtaagagtag
     2881 accacagact tatacatgga caagtagcat ttacatggac aaagttttta agcacagact
     2941 gtatattgat agcaagtgat gacttattaa aagatgaatt gagaatggca ggacttaaaa
     3001 tggctaaacc atctaatgtt aagttagtaa tgaaaagcat agcagattct ataaaagcac
     3061 ttaactcagg tgttactgat aaatacaact tattgatact ttgtgaatct gtagaagatg
     3121 tttatagact tgctaaagag gtaaaagcta ttaaatctat aaaccttggt ggaacaaaat
     3181 cagatgataa tcgtgaaaat atatctaaag cagtgcatgt atcaaaagat gatataaaga
     3241 tgattaaaga gttagattca gaaggcgtaa atgtatttgt acaattagtt cctgatgatg
     3301 atgctacaaa tgtaatgaaa ttaatataat tttaggggga tgaaaatgga atttacacaa
     3361 gttattttga taactctaat tgcattcttt gcatatatgc atagttttgt aggttctaca
     3421 atgcataata gacctatagt tgttgcacca cttgttggtt tagcactagg taatcttcat
     3481 acaggtatag taataggttc aacattagaa ttagtattta tgggtgcatt tcctgttggg
     3541 gcaagtaatc ctcctgattt tgtgtctggt tctattattg ccacagcgtt tgtaatatta
     3601 acgggtcaag atgtatcagc agcagttgta cttgcagttc ctatagcaac tctagtattg
     3661 cttatagata acttcttaat gacagtagta cttacatggg gagctcatat tgctgaccgt
     3721 tatgcagaag aggggaatat tgaaggcgta gaacgtgtgc aattattatt tggtatagga
     3781 aataaattaa tattagcaat aatagtaggt ataggatttt cattaggtgt tccagtaata
     3841 gaaaaaatac tttcatttat accatcatat gtaactcatg gaatggatgt tgcagcaggc
     3901 gtgattccag caataggttt tgccatgtta gctagaatga tgcttaataa gaaaacagta
     3961 gcatttttat tattaggatt tatattggtt gcttacctaa acataacagt aacaggagtt
     4021 gcattgtttg gattagcaat agctttaata tatgttaact ttgtaggaga gaaggaggta
     4081 atagtagatg acaacgagtt ctaagaagtt agagacaatt tcaccagata gtaaaataac
     4141 gaggaaagac ttttggaaat gttttagaag gtcattgaca ttagattcat cttggaacta
     4201 cgaacgtatg caaaatatag catatgcata catgatggca ccaataatcc gtagattata
     4261 taaagatgat aaagaaaaga aatctaaggc tttaaaaaga catttagaat ttatgtcagt
     4321 tactcctcat ataagtactc ttttggtagg tatatctgga gctatggaag aagaaaatgc
     4381 aaagaataaa gaatttgatg ctaatagtat aaatgctgtt aaatcaagtt taatgggtcc
     4441 agtttctggg ataggagatt catttttctg gggaacatta aagcttatag cagctggggt
     4501 tggtatagct ttagcttctc agggtaacat aatgggaccg attttattcc ttttaataat
     4561 aaatgttcct cattttatta tacgctatat atgtctagat aaaggattta agtatggaac
     4621 tcaattcttt aaagatgtga gtggttcaag tatagtttca aaagttatgg aagcagcttc
     4681 aatgttaggg cttatggtaa ttggaggtat gactgcatct aatgtaatgt taaaacttag
     4741 tgttaatgta ggtagtggag aatgggcaga gcctattcaa acttacttag accaaataat
     4801 gccatgtatg cttccagcta tgatattcgg tattatgtat tggttattag gaaaaaaggt
     4861 aaagacaact acgatattaa tttcagttat gattatttgt atagtattgg ctgctattgg
     4921 agtagtataa ataaaaaaat cccaagttca tttgaacgag ggattttttg actaaattaa
     4981 ataaaaaaga aagatggttc ataataattt ataagcattt atgtgatagt tataattagc
     5041 ttattaatta gttataaact caaaattaag cactacttta ttaaagttat tatattataa
     5101 tttcgaaata ctcacataat ctgtttagaa atttatcttc taatggggaa aaagttttct
     5161 ttttagagta aatgaagaaa aactttctag taaaatcaac atcctttatt ttataacatt
     5221 ttattttacc tgcattgaga taatcgatag ctgaaatgta cgatataaag gacactccaa
     5281 gacctaatct aaccatttcc ttaatagcct catttgattc tacatgagcg attacattta
     5341 atttacttac tggaaagtta tttttactaa gtgtgtttag tattaagttt cttgtacctg
     5401 acccttcttt tctcataata aaatttagat aagccaattc tcctatatcg atacaattat
     5461 ttttattatc aattttaaaa tctgaaggag tgataagaac aagttcatca tctaataagt
     5521 ctaggtactc tatttgagga ttattgattt tagaaccaac taatccaaag cttattcgtt
     5581 catttaaaat ttctgatata gcatattgag aatcataatg gcttatggaa aattttacat
     5641 ctggataact catagaaaaa ctcttcataa aatcaggtaa aatataagtt tcaggtatgg
     5701 aactacatgc tatatctata ataccttcta tttttcctga atattctttt atatcataaa
     5761 ttgcacgttt gcaattattc aatatatata tagcatgttc ataaagaatt tcaccagatt
     5821 ttgtgagtgt aataactttg ttgcttctat ttataagaac agtttctagc tctctctcta
     5881 aattctgtat gtgtgcgctt acagtaggtt gagttaaaaa aagttctctt gcagcctttg
     5941 aaaaactctg atgttttgca acagcaacaa atacttcaag ttgtttaaaa tccattattt
     6001 aatcctcctt aaattatcta aaactattat aacatatttg ataaattaat agtcaaaggt
     6061 gatttttaaa atagaaaacc tttaaatatt catggagttt ttgattgttt aggaataatt
     6121 tggtataatg ttgtaaggta cttacaataa aaatatcgta aattgaattt tcaaaattca
     6181 actttaaagt gttttttatt acagaaaaat tacaattagc ttcttaatct tattaaattc
     6241 aaataaatat tatataatta ttttataata taataaatat aattattgaa aaaagagata
     6301 actgtaaatt gagatataga taaatactat aaaatttaag aaaggaggat tgatttatga
     6361 agttaaaaag gtcattagtt tgtgtaacta ttttaggtat tatacttgtt ggatgtcata
     6421 aagaaaatac taaagagaaa aatcaagtag caagtaaagc aacacagcaa aaaactatga
     6481 caaaagttca aaatgatgtt aatgaaatta tgaataaaga ttataagtac attataaaga
     6541 atatgggtat accttataat acattttatt atataaaacc taaagtttta aaagaatcaa
     6601 atacaatgca agatataaat acatcaagtt atatgacatt agtttatcca aaatatactg
     6661 gaaatgatga actagatgga agtgctttat atgttgatat taacgaaaat aaagttgtca
     6721 atgttgaaac caattctttc tcatcacaag gtatatcagt cattgatgct gaatctagta
     6781 tagtgattga aaaaagtgac catgaaaaat cggctgtatc actagaaaat tttagacata
     6841 ttgacctggg agagtatgtt ggtgttgaag attccagaat aaatgaaatt gtaggagatg
     6901 cgaattatga tttaacagca tataatcatg aaggcagtaa agtagttaag agttatagat
     6961 tgaaagaaga taacaaaata ttaaaaaaag aggtacttac aataagtatt gtggataaca
     7021 aaataaaatc tataaaaact attgaaagtg ataaaattgt aaaaataata aaaggaactt
     7081 tattagaata aaatattcta aagcatatga tgctgtttca agatagaagg aaatttctag
     7141 ctgggaacag tttttttatt agatttataa aatttgaact gcacttatag ataactatta
     7201 gttataatta aatataagat aaaagtattt ccaactttta taatctataa tatattacat
     7261 agaaatatct taaataaaaa atatattggg gagtatttaa aatgatagaa ttagaagatg
     7321 ccataaaaat tattgaaaaa aatgttttac caataaacag ggtcaagata gtaaatctta
     7381 tagatgcagt aaataaggta gttgcagaag atatatattc aacaattgat agtcctccat
     7441 ttgataggtc accctatgat ggttatgcat ataatgcaaa ttctgaaaat agaaatttaa
     7501 aggttatagg gactttgtat gcaggagatg tatttaatag ggtattaaat aaaaatgaag
     7561 cagtgaagat aatgactggt ggaaagatac cacagggtgc aaattgtgta ataaaaaaag
     7621 aagatgtaac agttataggt gatatgataa ctttaaatat aaaattaaag gaatacgata
     7681 attacatatt taagggtgag gatataaaaa aggggcaatt gattattaat aaaaatcaga
     7741 atataggata tgatggtatt ggagtattag caagtcttgg aatatccaaa gtaactgttt
     7801 atgatgattt aaaagttgga attttgaata caggtacaga actacaagat ataaatgaga
     7861 ctttagagaa tggtaaaata tacgatagta atagatatac cttatattca agattattaa
     7921 aacttggaat acaagcagat acaatatgca attgttctga tgatatattg gagttagaaa
     7981 aaaaggtaaa gaatatgtta cagagtgtag attttttaat taccacaggt ggtgtttctg
     8041 ttggagataa agatttaatt ccagatgtat tcaaaaaaat aggggcaaca gaattatttt
     8101 ggaagataaa cattaaacct ggtggagctt gttatgtagg tacattggga gaaaagctat
     8161 tatttggtct ttctggtaat ccacatgcag caatagtggt atttgataat gtgatagttc
     8221 ctgttattga gtatttaaca tataagaagg gaacacatca tataaaagct atatttaaag
     8281 gagaatttaa taaattttca aataaagata gatttgtaag tggaaaacta tatacaagtg
     8341 aaggtaaatt gtatgtagag ctaaatgata agaaagatgc aaaagctaga ctatctgcaa
     8401 ctctaagaag taattgtatg attaagatta aaaagaatga gacaataaaa gaaaatcaat
     8461 tagtggatgt tgttattaga tagttttatg aaaggacaca attttatgta taatatattg
     8521 tcagttgtct catattcagg agtaggtaaa actacgttga ttgaaggcat aataaaagaa
     8581 ttaaataaaa aagggtatcg tgttggagtt attaaacata cctgtcatga ttttgatatg
     8641 gatgaggaag gaaaagatac ttataaacat agaaaatctg gggcgagaaa agtctgtata
     8701 atatcagata aacgagtagc atatattgaa gaactaaaag agaagaatcc attatataaa
     8761 atgattcaat tatatgaaga tatggacttg attatcatag aaggatataa aaattataga
     8821 tttaaaagac tggaagtaac tagaaaaggt aagtatgagg atattttatc taataagtct
     8881 gatttgatag gtattgttag tgatatagaa tataaattga atattgaaca gtttaattta
     8941 aatgattata aaaatatatc aaagtacata gagtcttgta taaaaaatga aattttaagt
     9001 ataagcaatg ttgaaataaa aaacatatta aaagaataat tttaagatgt tcttcttata
     9061 taaaatgata taattattta tataaataaa gatataagaa atttatcaat tttattttga
     9121 gttataaaat attaaagaaa ttgtaggagg acaatatatg aaaaaaatat tagtagcctg
     9181 tggagcagga atcgcaacat caacaatagt ttgtgacaga gtagaaagat tggttaaaga
     9241 aaataatata caagcagaag ttgagcaatg taaaattgct gaatgttcaa caaaacaaga
     9301 aggtgctgat ttaatcgtat ctactactat actaccaaca acttatgata tacctacaat
     9361 aaaggcaaca ggatatataa caggtataaa tacaacagca ttagataaaa agatattaaa
     9421 tgcattgaag tagttttgta tttataaaaa aaatttagta ttaaatatag agttgtatct
     9481 ttataaaatt ttaaatatag agatatagct ctttttatgt tgtttcaagt atacaactat
     9541 atgatataaa tcttcaattt tatgaaaaag attgttatta ctttgtaagt ttataaaaga
     9601 tttaaagtta ttgttatgtt atactaattt atgggtattt tttagaagta ctaatagaag
     9661 tactaataaa ggagggattt aatacttatg ataaaacaat ttgtcaaata ttataagcca
     9721 tataagaaaa tttttacact agaccttata gcagcttttc tattttcctt atgtgattta
     9781 gtttatccta tgataactag aaatataatg gatgacgttg ttccaaataa aaacttaaga
     9841 atgttagtag tttttgcagt agcacttata cttatattta tagcaaaagc agggcttaac
     9901 tattttatgc agtattgggg acatgttatt ggtgtagata tgcaagctga tatgagaaat
     9961 gaggtattta cacacttaca aagacttcca aatacgtatt ttgataataa caaatctgga
    10021 gtaactatgt ctagaatagt aaatgattta atggatataa cagaacttgc acatcatggg
    10081 cctgaagatt tattcatttc tatagtaatg ttggttggtt cattctttat acttatagat
    10141 ataaatattc ctcttacgct tataatattt gctatattac cgtttataat ttggtttgca
    10201 atagctaaaa aagataagat gaatattgct tttatgaaga gtagagttac tataggtgat
    10261 gtaaatgcta ctttagaaaa tagtatagca ggtatgaagg taactaagtc tttttgtact
    10321 gagaaagaag agttaaataa gtttgtaaga agcaataaat tatttagaag agcaagacaa
    10381 gattcttata aggtaatggc agaatattac tctggtatga atttatacat ggatatctta
    10441 gagtgggtag ttgtaatagc tggtggttat tttacttata taggaaagat tactttgggt
    10501 gattttgctg cttatatact atatgtaaaa atgtttatac aacctatgaa aaaattaata
    10561 aactttacag agcaatatca aaatggtatg acaggtttta aaagattcat agagataatg
    10621 gaacaagacc atcaaaaaga agctaaaaat cctattgaac ttgaaaatgt aaaaggtgac
    10681 attgagatag aaaatatatc tttcacatat gaagataaga ctcaagtttt ggataattta
    10741 agtttatcta taaaagctgg taagactata gcattagttg gaccttctgg aggaggaaag
    10801 actacattat gtaatttatt gccaagattt tatgaatttg ataagggtga tataaaaatt
    10861 gatggaaaga gtataaaaga tgttagttta aaatcattga gaaaaaatat aggtatagtt
    10921 caacaagatg tatttttatt tacaggaact ataagagata atattctttg tgggaaccct
    10981 aatgccacag atgaagaaat gatagctgct gctaaaaaag ctagaataca tgactttgta
    11041 gagactttgc cagatggata tgatacttat ataggagaaa gaggcgtaaa attatcaggt
    11101 gggcaaaaac aaagaatttc aatttcaaga atctttttaa agaatcctcc aataataata
    11161 ttggatgagg ctacatctgc tttagacaat gtaacagaaa gagaaattca agagtcttta
    11221 gaggaattaa gtaaagatag aacaaattta gttgttgcac atagattgac tactataaaa
    11281 aatgctgatg aaatcatagt attgacggat aaaggaattg aagaaagagg tactcatgaa
    11341 gagctagtaa acaaaaatgg agtatatagt aggttacaca ataactagta tacaaatttt
    11401 acaccatagc taacatacac attaaactta caaacatagg atatattaag attcataaag
    11461 acaattcatt ttatatttat aagatgtttt atgaataaaa tgtatcctat gttttatttt
    11521 ttgcaaatta catttaattc tctttgtaag aatagagtta aatagccaat ggtcgggata
    11581 acgtttttac aaaaagaata atttagaaaa ctaactttaa tttatggaat ttatcgaatt
    11641 ttatgaaaaa aatcgaattt tgacttgaaa tgtttttcaa aagtagtaaa ataatggata
    11701 actatacata cattttataa aaatatattt attatgaggg gggattttgt atggaggcaa
    11761 taacagcttt ttgtctagtt ttagtatcac tgacgatagg agatatagtt tcagcaaaaa
    11821 ctaaagcttt tgttccgtcg gtttttgtat cagcaattat atttatagta ggattttgga
    11881 cttttttccc agaaaatatt gtggacttag ccgctttagg aacaccatta gctcaattag
    11941 gtatgctttt gttaataact catatgggaa ctatgatgag tattaaggaa ttagcaggtc
    12001 aatggaaaac aatagtaata gccttggcag gaatagtagg aatatgtgta ggtgctttgg
    12061 cattagggac agtagtattt ggatgggata cagcagtaat agctacacct cctcttactg
    12121 gagggctagt tgcatctata atgatggcag atgctgctac agcaaaaggt cttgcaagtt
    12181 tatcagtact tgcaattttg atgtatgttg cacaaggatt tgcgggttat cctattacag
    12241 cattaatgct taaaaaagaa ggaaaaagac ttttatcaga ctttagaagt ggaaaagtaa
    12301 ctgtaaatac agatgaagaa aaagttaagg atttacctga acaaaaaagc agatttaaga
    12361 taataccaga cttaccagaa aaatatgata cgacatatat gataatactt agattaggat
    12421 ttgtagcatg gttagcagta caatttacaa atcttacaaa tgaagttgta tcaagatatg
    12481 ttatatgttt aatatttggt gtaatagcat cagaacttgg aatagttgat agaaaaccac
    12541 ttaataaatc tggttcattt ggatggttaa tgacatcatt gatggctttt atatttgcag
    12601 ggttagcaaa agctacacca gcaatgcttg ttgaaatggc agttcctctt gtaggaatca
    12661 taatatttgg tgtaataggt atgggaatat tctctattat agtaggtaag cttttaggtg
    12721 aaagtaaaga aatggctttt tcagtttcgc ttacatcact ttatggtttt ccaccaaact
    12781 atatattaac tgaagaggca gtaaaggctt tggctgaaac accagaagaa aaagaatttt
    12841 taatggataa gatgcttcca aagatgcttg ttggaggatt tactacagtt acaatagctt
    12901 ctgtagtaat agcaggtatc tttgtaaatc taatatagta tttaagtgat aaaaagtatt
    12961 ttaaataaga agaatgtgta ctacttggag gtatggaatg gatattaaag aaataacaaa
    13021 gagttataaa gactatgtaa ttaagcttag aagggaattt catgaaaatc ctgagaaaag
    13081 tatggaagaa gtgagaacat caaagagagt taaagaagaa cttgataaaa tagggatacc
    13141 atatgtatca gctggaggta caggtgtaat tgcaactata aaaggagcta atccaggaaa
    13201 gactgtagca cttaggggtg atatggatgc tctacaagtt gttgaatgca ctgatgtaga
    13261 atacaaatct aaaaatgaag gacttatgca tgcttgtggt catgatggtc acacatcaat
    13321 gcttctaggg gcagcaaaag tgttaaatga tattaaggat agtatcaatg gaactgtaaa
    13381 attatttttt cagccaggag aagaagttgg taaaggtgca agagctatga ttcaagatgg
    13441 tgctatggaa ggtgtagata gtgtgtttgg aatacatctt tggactgatg tagaatctgg
    13501 gaccatatcg gtagaagaag gaccaagaat ggcttctgct gatttcttta aaattactgt
    13561 aaaaggtaga ggtggtcatg gttcacttcc tcatcaaggt gttgatgcag tgcttgcaag
    13621 ttcagcaata gttatgaatc tacaatctat ggttagtaga gaagtaagtc ctctggaacc
    13681 attagttgtg agtgttggag ttttaaattc tggaactaga tttaacgtaa ttgcgagtga
    13741 agctgtctta gaaggtacaa taaggctatt taatccagag cttagaaagc aaattcctgg
    13801 aattttagaa cgtatagcaa aatctacagc agaagcatat agagcagatg ctgaattaga
    13861 gtatgggtac ttaacaccag cagttataaa tgacaaagaa tgttcaaaga tagctacaga
    13921 tgcagcaatt aagctatttg gagaagactg tataacacta tttgaaaaag taacaggagc
    13981 agaagattta gctgagttta tgaatatagc acctggtgca ctagcatttg taggagctag
    14041 aaatgagtca aaaggtgctt gttatcctca tcatcatgga tgctttaata tagatgaaga
    14101 tgctctagaa attggaactg ctttatatgt gcaatatgca gtggatttct taaataaata
    14161 attttaatat actataaaga aaaagcagag actaagttta tatctctgct tttttattgt
    14221 gtatattttt tataaatatt gactatataa aatatatatg atagcataaa gaaaatgata
    14281 atcaatatca attaaaaaga aggtgattta tttgaataat gtaatatttg catttttgat
    14341 aacattattg gctggtttat ctactggtat aggaagttgt atagcatttt ttgctaaaaa
    14401 aacaaatact aagtttcttt ctataagctt aggattttct gctggtgtta tgatttatgt
    14461 ttctatgata gaaatttttc ctaaagcaca agatgctttg acaaaatcta tgggagagaa
    14521 attaggaagt tggtcaactg taatagcttt ttttataggt atggctatca ttgctgttat
    14581 tgataagcta attccacaag aagaaaatcc acatgaaata aagaaaatgg agaatataga
    14641 agaaaagaat atcaaaaaaa ataagtctct tttgagaaca ggtatattta cagcaatggc
    14701 tatagcaatt cacaattttc ctgaaggatt agctacattt atatcagctc ttgatgatgt
    14761 tacaatagct attccgatag caattgcaat tgcaatacac aatataccag aaggtatatc
    14821 tgtatctgta ccagtttatt atgccacagg agataaaaag aaagcttttt attattcctt
    14881 tttatctggt atgtctgagc ctttaggagc gattattgga tatgtacttc ttagaaattt
    14941 tttaaatgat ataactcttg gaattgtatt tgcaattgta ggaggaatta tggtattcat
    15001 atcactagat gaattattgc catcagctag agagtacggg gaacatcatt tatctattta
    15061 tggattgatt gcaggaatgg gtgttatggc tattagttta ttactattta aatagctttt
    15121 taaatatatg attgtaacct aaaattagta tcaaaatgat ataattattt aaatagattt
    15181 ttaagctatt taaaaatatc attttggaga atgagtgcta tggaaaagaa aataaaattc
    15241 aaatttgact ggtatagttc acctgataag atagagaaat ggttagaaga tattgaagct
    15301 aaaggatata atttatataa gataaataaa tttggaggaa tattttattt tcataaggga
    15361 agtccaagaa aaataaaata cactgttgat tactactatg atgtagagaa taattatttt
    15421 gatgaacata aatcaaatgg ctggaatctt gtatttagga gtattggtag ttttatgggt
    15481 acatctgcat ggtatatatg gtcgaaagaa tacaaagaca tacctcctaa attatatagt
    15541 gattctgata aaaaaaacaa agtaaagtca attttattta tgaatatgtt tatattcttt
    15601 atgttagtag taatatttat atatttattg aatattgaaa taagaaattt atatagtggt
    15661 tatggagcat ctgtaactgc tattgtacta cagattatat tgttgatttt atatgcagta
    15721 caattatata ggagattaat gtattattta aggcttaaaa agtttaaata aataaaagaa
    15781 ggtgtcctaa aattattttt ttaatagttt aagacatctt cttttatcag taagtactgt
    15841 ttttaattat agtaatttct gtctttaaat tgaaatatca aataatagtt atatgggatt
    15901 gagtattgat aaaaataatt tgaaaatgtt atcctaaagt atcagatata caaaattatc
    15961 tttaaatata ttataaatat tttaattgta ttaactagaa attatgtaag gaggcatttt
    16021 atgattaaga tagcagtttg tgaagatgaa aaagaaacac aacttttaat agaagattac
    16081 cttgagaata tattaaaaga tataagtata gaatatgaga tacaaaagta tatatctgga
    16141 gaagagttac tggaaagtaa tttaaaagat atagatatat tacttcttga tataaaaatg
    16201 gaaaaattaa atggaatgga tactgcaaga aagattagag aagtagacaa tgaaatggaa
    16261 ataatatttg taacttcatt aatagattat gtgcaagaag gttatgaggt aagagcttat
    16321 aggtatttac taaagccaat agagttggaa gaactaaaaa aacatgtgct aacttgtatc
    16381 aaagatattg aaataaataa ggaaagtcat attacaataa aaaataagtc taatacatat
    16441 aagatttatt taaatgaaat aaaatatata gaagttcaaa aaaaagatat gctaatacac
    16501 acaataaaca agaattttga tataaaatat agtttaggca aaatagaaaa agaattaaat
    16561 ccatataaat ttataagatg ccataaaagt tttatagtaa atttaagata tgttgaaaat
    16621 ataaagccta atactgcaat actggaaagt ggagaagagg tacctattag tagatataga
    16681 tataaagaag ttaaggagaa attcttaaaa tttcttggtg atacaatatg ctaaacttta
    16741 tgactgagct attagtagtt ttatctatat ttatacagtt ttctataatg aacggaattt
    16801 taatttataa aaaggatagt aaaaaaaatg aaaaaatagt attctatatt gtcttgtttc
    16861 ttatttatat tataattcag ataagtagat tatttaatgg aggaaattac tttagtaata
    16921 taaactattt attttatata ttagcattta ttgcttgggg aggatactat aattattttt
    16981 ataagatggg ttatattaag tattccattt tcttttatct atttagtgta tattataaat
    17041 gtttggatgc tgttatatat agaatactat tttttgcaat tacaggt
//
LOCUS       DAEPIN010000077        16350 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.077, whole genome shotgun sequence.
ACCESSION   DAEPIN010000077 DAEPIN010000000
VERSION     DAEPIN010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16350
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.077"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            <1..369
                     /gene="tuf"
                     /locus_tag="KR250_003058"
     CDS             <1..369
                     /gene="tuf"
                     /locus_tag="KR250_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887863.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBH0068952.1"
                     /translation="DNIGALLRGVQRNEIERGQVLAKTGSVKAHTKFTAEVYVLKKEE
                     GGRHTPFFDGYRPQFYFRTTDVTGACKLPEGIEMVMPGDNVTMEVDLINSIVVEEGLR
                     FSIREGGRTVASGVVATIIE"
     gene            619..768
                     /gene="rpmG"
                     /locus_tag="KR250_003059"
     CDS             619..768
                     /gene="rpmG"
                     /locus_tag="KR250_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004402360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HBH0068953.1"
                     /translation="MRVKVTLACTECKQRNYNTTKNKKNNPDRIELQKYCRFCKKHTT
                     HKETK"
     gene            794..1015
                     /gene="secE"
                     /locus_tag="KR250_003060"
     CDS             794..1015
                     /gene="secE"
                     /locus_tag="KR250_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HBH0068954.1"
                     /translation="MAAQKNDGAKTKKRFSLFGYLKETKQELKRVTWPTKKELFKNTS
                     IVLTVVISCTILVWGIDTILSGALALLLK"
     gene            1035..1577
                     /gene="nusG"
                     /locus_tag="KR250_003061"
     CDS             1035..1577
                     /gene="nusG"
                     /locus_tag="KR250_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination factor
                     NusG"
                     /protein_id="HBH0068955.1"
                     /translation="MSELQEASWYVVHTYSGHENKVKATIEKAVKTRGMEDCIRQVVV
                     PTEEVVETTKTGKEKTRQRKVYPSYVLVKMIITDESWYVVRNTKGVTGFVGPGSKPVP
                     LSEDEVKAMGIDTTDPKVVNSDIDFEIGDTVKVSQGPFSGQIGNIEEIDLENREVKVC
                     INAFGKRTLFVIELEGIEKI"
     gene            1616..2041
                     /gene="rplK"
                     /locus_tag="KR250_003062"
     CDS             1616..2041
                     /gene="rplK"
                     /locus_tag="KR250_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HBH0068956.1"
                     /translation="MAKKVIGQIKLQIPAGKATPAPPVGPALGQHGVNIMGFTKEFNA
                     KTADQAGMIIPVVITVYQDRSFSFITKTPPAAVLIKKALNLKSGSGEPNKKKVAKMTS
                     AQVREIAELKMPDLNAASVEAAMSMIAGTARSMGVVIED"
     gene            2112..2810
                     /gene="rplA"
                     /locus_tag="KR250_003063"
     CDS             2112..2810
                     /gene="rplA"
                     /locus_tag="KR250_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HBH0068957.1"
                     /translation="MAKKGKRYAGALQKVDRTKFYDASEALTLVSDIAGAKFDETVEA
                     HIKLGVDSRHADQQVRGAVVLPHGTGKTKRVLVFAKGEKAKEAEQAGADFVGAEELVQ
                     KIQGENWFDFDIVVATPDMMGVVGRLGRVLGPKGLMPNPKSGTVTFDVAKAIDEIKAG
                     KVEYRLDKTNIIHVPVGKVSFGGEKLTENFTALMDAIIKAKPAAAKGQYLRSITVASS
                     MGPGVKINPARTAE"
     misc_feature    2862..2990
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            3033..3539
                     /locus_tag="KR250_003064"
     CDS             3033..3539
                     /locus_tag="KR250_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HBH0068958.1"
                     /translation="MRKAIEIKSEVVSEIVEKLQKSSAAVVVDYKGLTVEEVTELRKQ
                     MREAGVDYKVYKNTLVRRAAKEVGIEQFNDELLVGTNAIAFGYDDPVAPARILKGFMD
                     SHPKMKLKMGIVEGAFYDESKIVEMANIPSREVLIAKLLGSLKAPVSNFAYLIDAIAK
                     KAEGQEEA"
     gene            3597..3962
                     /gene="rplL"
                     /locus_tag="KR250_003065"
     CDS             3597..3962
                     /gene="rplL"
                     /locus_tag="KR250_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000159736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HBH0068959.1"
                     /translation="MTIEQILEAIENMKVLELNELVKAAEEKFGVSASAPVMVAGAAA
                     GGPAAEEKTEFDVVLTDVGSSKVGVIKAVREITGLGLKEAKEVVDNAPKTVKEGASKE
                     EADQIKEKLEAAGAKVEVK"
     gene            4354..5142
                     /locus_tag="KR250_003066"
     CDS             4354..5142
                     /locus_tag="KR250_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBH0068960.1"
                     /translation="MEKLQGKIAVVTAATKGIGLASAEILAKNGATVYLAARSEELAN
                     EVINKISAEGGCAKFVYFNAREEETFTSMIEEVVKKEGKIDILVNNFGSTNPSLDKDL
                     VTGDTDNFFDTVNTNLKSVYLPCKAAIPHMIKNGKGSIVNISSIGSVLPDLSRIAYCV
                     SKAAINSLTQNIATQYAKDNVRCNAVLPGLIATKAALDNMSPEFIKEFLKHVPLNRIG
                     EPDDIAKAVLFYASDDSSFITGDLLEVAGGFGLPTPQFADNILG"
     gene            5613..9329
                     /locus_tag="KR250_003067"
     CDS             5613..9329
                     /locus_tag="KR250_003067"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HBH0068961.1"
                     /translation="MPHPVTIGKRTRMSFSKIKEIADVPNLIEIQVDSYEWFLKEGLK
                     EVFDDISPIEDYTGNLILEFVDYSLDDKPKYDIEECKERDATYCAPLKVKVRLINKET
                     GEIKEQEVFMGDFPLMTERGTFVINGAERVIVSQLVRSPGVYYAEERDKTGKRLISST
                     VIPNRGAWLEYETDSNDVISVRVDRTRKQPVTVLLRALGIGTDAEIIDLLGEDERLSA
                     TLEKDNTKTVEEGLVEIYKKLRPGEPPTVESASSLLNALFFDPKRYDLAKVGRYKFNK
                     KLALCYRIMNKISAEDIINPETGEVFVKAGEKISYDLAKAIQNAGINVVNLLMDDDKK
                     VRVIGNNFVDIKSHIDFDIDDLNIKEKVHYPTLKEILDGYSDEEEIKEAIKSRIKELI
                     PKHILLDDIIASISYEFNIFYNIGNIDDIDHLGNRRIRSVGELLQNQVRIGLSRMERV
                     IKERMTVQDMEAITPQALVNIRPVSAAIKEFFGSSQLSQFMDQTNPLSELTHKRRLSA
                     LGPGGLSRERAGFEVRDVHHSHYGRMCPIETPEGPNIGLINSLGTYAKINEFGFIESP
                     YRKFDKETSTVTDEIHYLTADEEDLFVRAQANEPLTEDGKFVNHRVVCRTVNGAVEMV
                     PESRVDYMDISPKQVVSVATAMIPFLENDDANRALMGANMQRQAVPLVRREAPIIGTG
                     IEYRAAKDSGAVVVARNSGIAERVTADEIIIKREDGNRDRYNLLKFKRSNSGTCINQT
                     PIINKGDQIVKGDVIADGPATDLGEVALGRNCLIAFMTWEGYNYEDAILINERLVKED
                     RLSTIHIEEYECEARDTKLGPEEITRDIPNVGDSAIKNLDDRGIIRIGAEVDSGDILV
                     GKVTPKGETELTAEERLLRAIFGEKAREVRDTSLKVPHGESGIIVDVKVFTRENGDDL
                     SPGVNELVRCYIAKKRKIKVGDKMAGRHGNKGVISRVLPEEDMPFMENGTPLDIILNP
                     QGIPSRMNIGQVLEVHLGLAAKTLGWYVATSVFDGANEYDIMDALEEAGYPRDGKLTL
                     YDGRTGESFDNRITVGYMYYLKLHHLVDEKLHARSTGPYSLVTQQPLGGKAQFGGQRF
                     GEMEVWALEAYGAAHILQEILTVKSDDVVGRVRTYEAIVKGENIPEPGIPESFKVLIK
                     ELQSLCLDVKVLTDEDQEIEVRESVDEDDTIGEFELDVVNHMGEVEESNIIEEIEDDF
                     AENAEDEDIENLEEFTEDDLFEEEIDFDSDDFDM"
     gene            9371..12856
                     /gene="rpoC"
                     /locus_tag="KR250_003068"
     CDS             9371..12856
                     /gene="rpoC"
                     /locus_tag="KR250_003068"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HBH0068962.1"
                     /translation="MFELNNFESIKIALASPEKIRQWSRGEVKKPETINYRTLKPEKD
                     GLFCERIFGPQKDWECHCGKYRRVRYKGVVCDRCGVEVTKSKVRRERMGHIELAAPMS
                     HIWYFKGIPSRMGLLLDMSPRSLEKILYFASYVVVDPGETGLNEKQLLTEKEYRTALE
                     KYGYTFTVGMGAEAVKTLLQNIDLEQQSKDLRAELKDSTGQKKVRTIRRLEVVEAFKK
                     SGNKPEWMILDAIPVIPPDLRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLLELGA
                     PDIIVRNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSDMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPELKFYQCGLPKKMALELFKPFVMDKLVKEGYAHNIKSAKSIVEK
                     VKPEVWDVLEDVIKSHPVLLNRAPTLHRLGIQAFEPILVEGKAIKLHPLVCTAYNADF
                     DGDQMAVHVPLSVEAQAEARFLMLSVNNILAPKDGSPITTPSQDMVLGCYYLTIEAQD
                     GAKGTGMVFKDFNELLLAYYNKSVHLHALVKLKVTLEDGRSSLVESTVGRFIFNENIP
                     QDLGFVDRKENPFALEVDFLADKKSLGKIIDKCFRKHGNTETAELLDYIKALGFKYST
                     LGGITVAVDDMSVPEEKKVFIAEAEAKVDKYEKAYRRGLISDEERYEKVIETWTETTD
                     KVTDALMGGLDRLNNIYIMAHSGARGSKNQIRQLAGMRGLMANASGKTVEIPVKSNFR
                     EGLSVLEYFTSSHGARKGLADTAIRTAESGYLTRRLVDVSQDVIVREIDCGTEDTTEI
                     YAIKEGNEVIEEIYDRIVGRYTIDPILNPETGEVIVEADSMIQEDEAETIVALGIEKI
                     RIRTVLNCKTNHGVCSKCYGRNLATGKEVNIGEAVGIIAAQSIGEPGTQLTMRTFHTG
                     GVAGADITQGLPRVEELFEARKPKGLAVITEVSGRVEIDETGKRKEVNVIPEEGETQT
                     YVIPYGSRLKVKQGQMLEAGDPLTQGFINPHDIVRVNGVKGVQEYIVKEVQRVYRLQG
                     VDVNDKHIEVIVRQMLSKVKVEDPGDTDLLPGGYEDVLTFNECNKDAIDKGLRPAVAK
                     RVLLGITKASLATDSFLSAASFQETTRVLTEAAIKGKEDHLIGLKENVILGKLIPAGT
                     GMKKYRNIAVEKIED"
     gene            13145..13567
                     /gene="rpsL"
                     /locus_tag="KR250_003069"
     CDS             13145..13567
                     /gene="rpsL"
                     /locus_tag="KR250_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HBH0068963.1"
                     /translation="MPTINQLVRKSRKALEKKSTAPALQKGYNSLNKKVTDASAPQKR
                     GVCTSVKTVTPKKPNSALRKVARVRLTNGIEVSAYIPGEGHNLQEHSVVLIRGGRVKD
                     LPGVRYHILRGTLDTAGVDKRRQSRSKYGAKRPKEAKK"
     gene            13691..14161
                     /gene="rpsG"
                     /locus_tag="KR250_003070"
     CDS             13691..14161
                     /gene="rpsG"
                     /locus_tag="KR250_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HBH0068964.1"
                     /translation="MPRKGNIPKREVLPDPMYGSKVVTKLINNLMVDGKKGKSQRIVY
                     DAFAIVAEKTGEEALEVFNKAMDNIMPVLEVKARRVGGANYQVPIEVRPERRQTLGLR
                     WLVKYTRARGEKGMVEKLAKEIMDAANNTGASVKKKEDTHKMAEANKAFAHYRW"
     gene            14210..16276
                     /gene="fusA"
                     /locus_tag="KR250_003071"
     CDS             14210..16276
                     /gene="fusA"
                     /locus_tag="KR250_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HBH0068965.1"
                     /translation="MARKFPLERTRNIGIMAHIDAGKTTTTERILFYTGQTHKIGETH
                     EGASQMDWMEQEKERGITITSAATTASWKDHRINIIDTPGHVDFTVEVERSLRVLDGS
                     VAVFCAKGGVEPQSENVWRQAETYGVPRIAFVNKMDILGADFYNVVSMMKSRLNSNAV
                     PMQLPIGKEDSFIGIIDLLKMDAVIYKDDLGVEMEETDIPEDMKELAAEWREKLVESV
                     AETDEELMMKYLEGEELTIDELKVAIRKATIACEMNPVFCGTAYRNKGVQLVIDAVLD
                     YLPAPTDIPAIKGILADGEEAERHSSDEEPFSALAFKIMTDPFVGKLAFFRVYSGTLE
                     SGSYVLNATKNKRERIGRILQMHANTREEITKVYAGDIAAAVGLKDTTTGDTLCDPAN
                     PIILESMEFPEPVISVAIEPSSKAAQEKMGIALQKLAEEDPTFTVKTDQETGQTIISG
                     MGELHLEIIVDRLLREFKVEAKVGAPQVAYRETITQPVDVEYKYSKQSGGRGQYGHVK
                     IRVAPQEPGEGYKFTNKTVGGSVPKEYVGPVDMGIQGAMQSGIVAGYPVVDVAVELYD
                     GSYHEVDSSEMAFKMAGSMAFKDAMKKGNAVLLEPYFKVEVVTPEDYMGDVMGDLNSR
                     RGLIQGMEARSGAQVINAFVPLSEMFGYSTDLRSSTQGRATYTMIFDHYEQVPASVAK
                     KIAEGK"
BASE COUNT         6411 a         2033 c         3339 g         4567 t
ORIGIN      
        1 gataatatag gagcattatt aagaggagta caaagaaacg agatagaaag aggacaagta
       61 ctagcaaaga ctggatcagt aaaggcacac acaaagttta cagcagaagt atatgtactt
      121 aaaaaagaag agggtggaag acatacacca ttctttgatg gatatagacc acaattctat
      181 ttcagaacaa cagacgtaac aggagcttgt aagttaccag aaggaataga gatggtaatg
      241 cctggagata acgtaacaat ggaagtagac ttaataaact caatagttgt agaagaggga
      301 ttaagattct caataagaga aggtggaaga acagtagctt caggagttgt tgctacaata
      361 atagagtaat tctttactaa actgatggat taaaaggaat aacaaaaaga ggggttatgc
      421 tcctcttttt ttaacataga aatacaaaaa cattgacaca gcataggatt ttgtgataac
      481 ttatacaagg taatgattat aggaagttaa taatatcttg aatactgtag atttttaata
      541 aatacatatt ttaaagatat tatattcaca tatattaaca atatatatta ttgtattaca
      601 aagttggagg tgtatcagat gagagttaaa gtaactttag catgtacaga gtgtaagcaa
      661 agaaactaca acactactaa gaataagaaa aataatccag acagaataga attacaaaaa
      721 tattgtagat tctgtaaaaa gcatacaact cataaagaaa caaaatagtt ttttataagg
      781 atgtgagtat ggaatggctg cccaaaaaaa tgacggtgca aaaactaaaa aaaggtttag
      841 tttgtttgga tacttaaaag aaactaaaca agaattaaaa agagttacat ggccgacaaa
      901 aaaggaactg tttaaaaaca caagtatagt tttaactgta gttatttcat gcactatatt
      961 agtatggggt atagacacta tattatctgg tgcactagcg ttattactaa aatagacgaa
     1021 aagaggtaag ttaaatgtca gaattacaag aagcaagttg gtatgtagtt catacctatt
     1081 cgggacatga aaataaagtt aaagctacaa ttgagaaagc agttaaaaca agaggtatgg
     1141 aagactgcat aagacaagta gttgttccaa cagaagaagt agtggaaact actaaaactg
     1201 gaaaagaaaa aactagacaa cgtaaagttt atccaagtta tgttttggtt aaaatgataa
     1261 taactgatga atcttggtat gtagtaagaa atactaaggg agttacaggt ttcgttggac
     1321 ctggttcaaa accagtgcca ttaagtgaag acgaagtcaa agctatgggg atagacacaa
     1381 cagaccctaa agtagtaaat agcgatattg attttgaaat aggagataca gtaaaagtat
     1441 ctcaaggacc atttagtgga caaataggta acatagaaga aattgactta gaaaatagag
     1501 aagtcaaagt atgcataaat gcatttggta aaaggactct atttgtaata gagcttgaag
     1561 gtatagaaaa aatctaatac tatattatag aaaaatctga ggaggtgcgc tcaaaatggc
     1621 taaaaaagtt ataggtcaaa taaaattaca aatacctgca ggaaaggcta ctccagcgcc
     1681 accagttgga ccagcattag gacaacatgg tgttaatata atgggattca caaaagaatt
     1741 taatgctaaa actgcagatc aagctggaat gataatacca gttgttataa ctgtatatca
     1801 agatagatca ttcagtttta taacaaaaac tccaccagct gcagttttaa ttaaaaaagc
     1861 attaaactta aaatcaggtt ctggagaacc aaacaagaaa aaagttgcta aaatgacttc
     1921 agctcaagta agagaaatag ctgaattaaa gatgcctgac ttaaatgctg catcagtaga
     1981 agctgctatg agtatgatag ctggtactgc tagaagcatg ggtgttgtaa tcgaagacta
     2041 gttaaaagat acactttttt ttataaagtg ggaggctaga agccgaaatt aaccacgagg
     2101 aggaaaataa aatggctaaa aaaggtaaaa gatatgcagg tgcattacaa aaagtagata
     2161 gaactaaatt ttatgatgca tcagaagcat taacattagt ttcagatata gctggagcta
     2221 aatttgatga gacagtagaa gcacacatta aattaggtgt tgactcaaga catgctgacc
     2281 aacaagtaag aggtgcagtt gtattacctc atggaactgg taaaacaaaa agagttttag
     2341 tttttgctaa aggtgaaaaa gctaaagaag ctgaacaagc tggtgctgac tttgtaggag
     2401 ctgaagaatt agttcaaaaa atacaaggtg aaaactggtt tgactttgat atagtagttg
     2461 ctactccaga tatgatgggt gtagtaggta gattaggtag agtattagga cctaaaggtt
     2521 taatgccaaa ccctaaatca ggaacagtta catttgatgt agctaaagct atagatgaaa
     2581 taaaagctgg taaagttgaa tacagattag acaaaactaa cataatacac gttccagtag
     2641 gaaaagtatc atttggtgga gaaaaattaa ctgaaaactt tactgcatta atggatgcta
     2701 taataaaagc taaaccagct gctgctaaag gacaatattt aagaagtata acagtagctt
     2761 cttcaatggg acctggagtt aaaataaacc cagcaagaac agctgaataa taaaactgtt
     2821 gacacaggtt gacaaaaatg ttattatatc acttgtcaat gtaaaaaaga atagatttgc
     2881 cgtagatagt gggtgcgaaa gcttaatatc ccgccgaggt tttagataaa ttgagattac
     2941 taaagctttt gatgtctact tttggcaaca aaagcttttt ataatgttgc ggtaaatact
     3001 aagcctattt ggtgaaggag gtgcacatct atatgagaaa agctatagaa attaaatcag
     3061 aagttgtttc tgagatagtt gaaaagttac aaaaatcttc tgctgctgtt gttgtagatt
     3121 acaaaggatt aacagttgaa gaagtaactg agttaagaaa acaaatgaga gaagctggtg
     3181 tagattacaa agtatacaaa aatactttag ttagaagagc agctaaagaa gttggtatag
     3241 agcaatttaa tgatgaatta ttagttggaa ctaatgccat agcatttgga tatgatgatc
     3301 cagttgctcc agctagaata ttaaaaggat tcatggattc tcatccaaaa atgaaattaa
     3361 agatgggtat tgtggaaggt gcattctatg atgaatctaa gattgttgaa atggctaaca
     3421 taccatcaag agaagttctt atcgctaaat tacttggtag cttaaaagct ccagtatcaa
     3481 acttcgcata cttaatagat gcaatagcga aaaaggcaga aggtcaagaa gaagcttaat
     3541 tgcttaacta aaaaattata aatattaatt tgaaataaaa ttcggaggtg ctaaaaatga
     3601 caatagaaca aatattagaa gctatagaaa atatgaaagt tttagaatta aatgaattag
     3661 ttaaagctgc tgaagaaaaa ttcggtgtat cagcttcagc tccagtaatg gtagctggtg
     3721 cagctgctgg tggaccagct gctgaagaaa aaactgagtt tgatgtagta ttaactgacg
     3781 ttggttcttc aaaagttgga gttataaaag cagttagaga aataacagga ttaggattaa
     3841 aagaagctaa agaagtagtt gacaatgctc ctaagacagt taaagaagga gcttctaaag
     3901 aagaagctga tcaaataaaa gagaaattag aagctgctgg agctaaagta gaagttaagt
     3961 agtttcttta aaaagatacc cattgggtat ctttttttat ttataaaaaa ctataaagaa
     4021 agtatttgga attataaaga aagtattttt ataaaataaa aaaaatttat ataatagtac
     4081 taaattaagg gtatcatata tggtgtaatg gtattttaat ttaacattat tatcaaatta
     4141 aaatataaac aattgttgta atgtctatat ataaagtata taattaaact aaaatgtaaa
     4201 attttacatt ttagtttaaa aaaatacacg aatgttttat aaataaaatg taataaataa
     4261 ttaattaaca aatataaatt ttaaaataag ttagatattt agaaaaatcg aaatttgtag
     4321 gatttaaagg acatattcaa ggaggagtta ataatggaaa aattacaagg aaaaattgca
     4381 gtagttactg cagcaacaaa aggtattgga ttagcatcag cagagatatt agcaaaaaat
     4441 ggagcaactg tgtacttagc agctcgttca gaagaattag ctaatgaagt tataaataaa
     4501 ataagtgcag aaggtggttg tgctaagttt gtttacttta atgctcgtga agaagagacc
     4561 tttacctcaa tgatagaaga ggtagttaaa aaagaaggta agatagatat acttgtaaac
     4621 aattttggtt caacaaaccc ttctcttgac aaagaccttg taactgggga tacagataat
     4681 ttctttgaca cagtaaatac taatttaaaa agtgtgtatt taccatgtaa agcagcaatt
     4741 cctcatatga taaagaatgg aaaaggaagt atagtaaata tatcaagtat aggttcagta
     4801 ttacctgatt tatctagaat agcttactgt gtatcaaaag cagcaatcaa ctcattaact
     4861 caaaatatag ctacacaata tgctaaagac aatgttagat gtaatgctgt acttccaggg
     4921 cttattgcaa ctaaagcagc attagacaat atgtctccag agttcataaa agaattttta
     4981 aagcatgttc ctttaaatcg tataggggaa ccagatgata tagcaaaagc tgttctattc
     5041 tatgctagtg atgactcatc atttataaca ggggatttac ttgaagttgc aggaggattt
     5101 ggtttaccta ctccacaatt tgcagataat atattaggat aattaaatta ctaatatgca
     5161 atgaaagctt atgcattagt tatagtagaa atgatatttt tataaaggcc atagatataa
     5221 tttatattgg aaaggtttga ggttcaaaat tccaagttaa atttatttat ggtctttatt
     5281 ttgtatattt taataaattg tatatatttg tataaatgta attatttaca atcattttaa
     5341 tatggaatta taattaaata ggggggatgg tacttgtata aaatttttta tagaaaatta
     5401 gacctataaa tgaaaaatat ggattttata ttggaaaatt gtacataata cataatatat
     5461 gcttaaaata atttacaaaa aatcaaaaat aatgcatagt ttacatataa gataaaaaat
     5521 atattgaaaa atacttgaca ttatgctaaa attattagat gcgttagaat gaactattgt
     5581 tttattttta attattgaga ggtgaaaagt gaatgccaca tcctgtcacg ataggtaaaa
     5641 gaactagaat gagcttttct aaaattaagg aaatagctga tgtgccaaat cttatagaaa
     5701 ttcaagtaga ttcctatgag tggtttttaa aagaaggttt aaaagaagtg tttgatgata
     5761 tttcaccaat agaagattat actggtaatc ttatattgga atttgtcgac tattctctag
     5821 atgataaacc aaagtacgat atagaagagt gtaaagagag agatgctaca tattgtgcac
     5881 ctctaaaggt taaggtaaga cttataaata aagaaacagg tgagataaaa gaacaagaag
     5941 tgtttatggg tgacttccct ttaatgacag aaagaggaac tttcgttata aatggagcag
     6001 aaagagttat agtaagtcaa ttagttagat ctcctggtgt ttactatgcg gaagaaagag
     6061 ataaaacagg taagagattg atttcatcta ctgtaattcc taatagagga gcatggttag
     6121 aatatgaaac agattctaat gatgtaatat ctgtaagagt tgatagaaca agaaaacaac
     6181 cagttacagt tttacttaga gctttaggaa taggaacaga tgcagagata attgaccttt
     6241 taggagaaga tgagagatta tctgctacat tagaaaaaga taatactaag acagtagaag
     6301 aaggtcttgt agagatatac aaaaaattaa gaccaggtga acctcctact gtagaaagtg
     6361 catcatcttt attaaatgct ttattctttg acccaaaaag atatgaccta gcaaaagttg
     6421 gtagatataa attcaataag aaacttgctt tatgttacag aataatgaat aaaatttcag
     6481 cagaagacat aataaatcca gaaactggag aagtatttgt aaaagctgga gaaaaaataa
     6541 gctatgattt ggctaaagcc atacaaaatg caggtataaa tgtagtaaat ctactaatgg
     6601 atgatgataa aaaagttaga gttataggaa ataattttgt ggatattaag tctcacattg
     6661 attttgacat agatgattta aacataaaag aaaaagtaca ttatccaact ttaaaagaaa
     6721 ttttagatgg atatagtgat gaagaagaaa taaaagaggc tataaaatca agaattaaag
     6781 aacttatacc aaaacatata ttattagatg atataatagc ttcaataagt tatgagttca
     6841 atatattcta taatatagga aatattgatg atatagatca cctaggaaac agaagaataa
     6901 gatctgtagg tgaattgtta caaaatcaag ttagaattgg tctttcaaga atggaaagag
     6961 ttataaaaga aagaatgacg gttcaagata tggaagctat aacgcctcaa gcgttagtta
     7021 acataagacc agtttcagct gcaataaaag aattttttgg tagttctcag ttatctcagt
     7081 tcatggatca aacaaatcct ctatctgagt taacgcataa gagaagatta tcagcccttg
     7141 ggcctggagg tctttcaaga gaaagagctg gattcgaagt gcgtgacgtt caccattcac
     7201 actatggtag aatgtgtcca atagagactc cagagggacc aaatatcggt cttataaact
     7261 ctctaggaac ttatgcaaaa ataaatgaat ttggatttat agaatcacca tatagaaaat
     7321 ttgataaaga aacatcaaca gttactgatg aaatacatta tttaactgct gacgaagaag
     7381 atttattcgt aagagctcag gcaaatgagc cattaacaga agatggtaaa ttcgtaaacc
     7441 atagagttgt ttgtagaact gtaaatggtg ctgttgaaat ggttcctgaa agtagagtag
     7501 attacatgga tatatctcct aagcaggttg tatctgttgc cactgctatg atacctttct
     7561 tagaaaatga tgatgccaac cgtgccctta tgggagcgaa catgcagcgt caggcagtgc
     7621 ctctagttag aagagaagca ccaattatag gaactggtat agaatacaga gcagcaaaag
     7681 actctggagc agttgttgtt gctagaaatt ctggtatagc agaaagagta acagcagatg
     7741 aaataattat aaaaagagaa gatggaaaca gagatagata taatctactt aagtttaaac
     7801 gttcaaactc aggtacttgt ataaatcaaa cacctataat aaataagggt gaccagatag
     7861 taaaaggtga tgttatagca gatggtccag caactgattt aggagaagtt gcacttggaa
     7921 gaaactgtct tatagcattc atgacttggg aaggttataa ctacgaggat gctatattaa
     7981 taaatgaaag attagttaaa gaagatagat tatcaacaat tcatatagaa gaatatgaat
     8041 gtgaagctag agatacaaaa ctagggccag aggaaataac tagagatata cctaatgttg
     8101 gagatagtgc aattaagaac ttagatgaca gaggtataat aagaataggt gcagaagtag
     8161 actcaggtga tatattagtt ggtaaagtaa ctccaaaagg agaaactgaa cttactgctg
     8221 aggaaagatt acttcgtgca atatttggag aaaaggctag agaagttaga gatacttcac
     8281 ttaaagtgcc tcatggtgaa tctggtataa tagttgatgt aaaagtattt actagagaaa
     8341 atggagatga tttatctcca ggagtaaatg aattagtaag atgctatata gctaaaaaga
     8401 gaaaaataaa agttggagat aaaatggctg gtcgtcatgg taataaaggg gttatctcaa
     8461 gagtattacc tgaagaagat atgccattca tggaaaatgg aacaccacta gacataatac
     8521 ttaacccaca aggtatacca tcacgtatga acattggtca ggttttagaa gttcatttag
     8581 gacttgctgc taagacatta ggatggtatg tagcaacatc agtattcgat ggtgctaatg
     8641 agtatgacat aatggatgct cttgaagaag ctgggtatcc tagagatggt aaattaactt
     8701 tatatgatgg tagaacaggt gagtcttttg acaacagaat aactgttgga tatatgtact
     8761 acttgaaact acatcactta gtagatgaaa aactacatgc aagaagtact ggaccatact
     8821 cattagttac tcaacagcca ctaggtggta aagcacaatt tggtggacaa agatttggag
     8881 agatggaggt ttgggctctg gaagcatatg gagctgctca catacttcaa gaaatactta
     8941 ctgttaagtc tgatgacgtt gtaggtcgtg ttagaacata tgaagctata gttaaaggtg
     9001 aaaatatacc tgaaccagga atcccagaat catttaaggt tcttataaaa gaacttcaaa
     9061 gtttatgctt agatgtaaaa gtattgacag atgaagacca agaaatagaa gtaagagaat
     9121 ctgtggatga agatgataca ataggtgaat ttgagctaga tgttgtaaat catatgggag
     9181 aagttgaaga aagtaatata atagaagaaa ttgaagatga ttttgcagaa aatgcagaag
     9241 atgaggatat agagaattta gaagaattca ctgaagatga tttatttgaa gaagaaatag
     9301 attttgatag tgatgacttt gatatgtaaa tttaattaat gtaaatctca aatagaaggg
     9361 agagaactcc ttgtttgaat taaacaattt cgagtcgata aaaatagcat tggcttctcc
     9421 agaaaaaata agacaatggt ctaggggaga agttaaaaag ccagaaacta taaattaccg
     9481 tactttaaaa ccagaaaaag atggtctttt ctgtgaaaga atatttggac cacaaaaaga
     9541 ctgggagtgc cactgtggta aatatagaag agttagatat aaaggtgtag tttgtgatag
     9601 atgtggagta gaagtaacta aatcaaaagt aagaagagag agaatgggac atatagagct
     9661 agcggctcct atgtctcaca tctggtactt caaaggtata ccaagtagaa tgggactttt
     9721 acttgatatg tcaccaagat cgttagaaaa aatattatac tttgcctcat atgtggtagt
     9781 tgatccagga gagactggat taaatgaaaa acaattgctt acagaaaaag aatacagaac
     9841 tgctcttgaa aagtatggat atacttttac tgtaggaatg ggtgctgaag ctgtaaagac
     9901 attactacaa aatatagatt tagagcaaca aagtaaagac ctaagagcgg agttaaaaga
     9961 tagtacaggg caaaagaaag ttagaacaat aagaagatta gaagttgtag aggcatttaa
    10021 aaagtctgga aataaaccag aatggatgat tttagatgca ataccagtaa taccaccaga
    10081 tttaagacca atggtacaac ttgatggtgg aagatttgcg acttcagacc taaatgattt
    10141 atatagaaga gttataaata gaaacaatag acttaaaaga ttattagagc ttggagctcc
    10201 agatataatt gtaagaaatg aaaaaagaat gcttcaagaa gctgttgatg cattgataga
    10261 taatggtaga agaggtagac ctgtaacagg acctggaaat agaccactta aatctttatc
    10321 agatatgtta aaaggtaagc aaggtcgttt ccgtcaaaac ttacttggta agcgtgttga
    10381 ctactcagga cgttctgtta tagttgttgg accagaactt aaattttatc aatgtggact
    10441 tccaaagaag atggcattag aattattcaa gccatttgtt atggataaat tagttaaaga
    10501 aggttatgca cataatataa aaagtgcgaa atctatagta gaaaaagtta agccagaagt
    10561 ttgggatgtt ttagaagatg ttataaaaag tcatccagtt cttcttaacc gtgcaccgac
    10621 tctacataga ttaggtatac aagcatttga accaatctta gttgaaggta aggctataaa
    10681 actacatcct cttgtatgta cagcttataa tgcagacttt gatggtgacc aaatggcagt
    10741 tcatgtacct ttatcagtag aagcacaagc agaagcaaga ttcttaatgc tttctgtaaa
    10801 taatatactt gctcctaaag atggttcacc tataactact ccatctcagg atatggtttt
    10861 aggttgctat tatctaacaa tagaagccca agatggagct aaaggaacag gtatggtatt
    10921 taaagacttt aatgaactat tacttgctta ttacaataaa tcagttcatc tacatgcatt
    10981 agtgaaactt aaggtaacac ttgaagatgg aagaagttca ttggttgaaa gtactgttgg
    11041 tagatttata tttaatgaaa atatacctca agacttaggt tttgtagata gaaaagaaaa
    11101 tccatttgca cttgaagttg atttcttagc agataaaaaa tctcttggta aaataataga
    11161 taaatgcttt agaaaacacg gaaatacaga aacagctgaa ttactagatt atataaaagc
    11221 tctaggattt aaatattcta cattaggtgg cataacagtt gctgttgatg atatgagtgt
    11281 cccagaagaa aagaaagtat ttatagctga ggcagaagct aaagttgata agtatgaaaa
    11341 agcatacaga agaggtctaa tctctgatga agaaagatat gaaaaagtta tagagacatg
    11401 gacagaaaca actgataaag ttactgatgc tcttatgggt ggactagata gattaaataa
    11461 tatatatata atggcacatt caggagccag aggttctaaa aaccaaatta gacagctggc
    11521 aggtatgcgt ggtcttatgg caaatgcatc tggtaaaaca gttgagatac cagttaaatc
    11581 taatttccgt gaaggtttat cagtactaga atactttaca tcttcacatg gtgccagaaa
    11641 aggtcttgcc gatacagcta tacgtacagc tgaatctgga tatttaacaa gaagacttgt
    11701 tgatgtaagt caagatgtta ttgtaaggga aatagattgt ggaacagaag atactacaga
    11761 gatttatgct ataaaagaag gaaatgaagt tatagaagag atatatgata gaattgtagg
    11821 aagatatact atagacccta tattaaatcc tgaaactgga gaagttatag ttgaggctga
    11881 ttcaatgata caagaagatg aagcagaaac tatagtagct ttaggaattg aaaaaattag
    11941 gataagaaca gttcttaact gtaaaactaa tcatggagtt tgttctaagt gttatggtag
    12001 aaacttggca acaggaaaag aagttaatat aggtgaagca gttggtataa tagcagctca
    12061 atctatcggt gagccgggta ctcagcttac aatgcgtaca ttccatactg gaggagttgc
    12121 aggagctgat ataactcaag gtctaccaag ggttgaagaa ttatttgaag ctagaaaacc
    12181 aaaaggatta gctgtaataa ctgaagtatc tggtagagtt gaaattgatg aaacaggaaa
    12241 gagaaaagaa gtaaatgtaa taccagaaga aggcgagact caaacatatg taattccata
    12301 tggttctaga ttaaaagtta agcaaggtca aatgctagag gctggagacc ctctaacaca
    12361 aggatttata aatcctcatg atatagtaag agtaaatggt gttaagggag ttcaagaata
    12421 tatagttaaa gaagttcaaa gagtgtatag acttcaaggg gttgatgtta atgataagca
    12481 tatagaagtt atagtaagac aaatgctatc taaagtaaaa gttgaagacc caggagatac
    12541 agatttattg ccaggtggat atgaagatgt attaacattc aatgaatgta ataaagatgc
    12601 tatagataaa ggtctaagac cagcagttgc taaaagagtt ttacttggta taactaaagc
    12661 atctcttgca actgattcat tcttatcagc agcttctttc caagaaacaa caagagtatt
    12721 aacagaggca gcaataaaag gtaaagaaga ccacttaata ggacttaaag aaaatgttat
    12781 attaggtaag ttaataccag caggaacagg aatgaagaaa tatagaaata tagctgttga
    12841 aaaaattgaa gattaatagc atatagagta aagaaatctt gacaaccatt atttgtggtg
    12901 ttaaaatagt aaagtgtgtt gtttaaaagt acactaaggg cttgaacaag ttcttagtga
    12961 cttttagatg gcaataaatg agaactaaaa agcatcaagc tagaaaagtg ctaatatcac
    13021 atatttatgt gttatagaca ttaaattgta agtaacgaat gttacaatca gtcagtatga
    13081 atattcatat tgatgtagtt ggcggtcact gataagatcg aaaatttgga aggaggtgca
    13141 gatgatgcca acaattaacc aattagttcg taaaagtaga aaagcgctag agaagaaatc
    13201 tactgctcca gcattacaaa aaggttacaa ctctttaaat aagaaagtaa ctgacgctag
    13261 tgccccacaa aaaagagggg tttgtacttc agtaaaaaca gttactccta agaagcctaa
    13321 ctcagcttta agaaaagttg ccagagttag attaactaat ggtatagaag tttctgctta
    13381 tataccagga gaaggacaca acttacaaga gcatagtgtt gttcttataa gaggaggaag
    13441 agtaaaagac cttccagggg ttagatacca tatactaaga ggtactctag atacagcagg
    13501 tgttgataag agaagacaat caagatctaa atacggtgcg aagagaccta aagaagctaa
    13561 aaagtaaaaa caattaatga ttgattatag ttcggtgctt taaatatata tatatttacg
    13621 gcatcacggc attaaatgat ttatgtcgag tacctatgat ttaagttaaa taattaagga
    13681 gggaagcgaa atgccaagaa aaggtaatat tccaaaaaga gaagttttac cagatcctat
    13741 gtatgggagt aaggtagtta ctaaattaat aaataatcta atggtagacg gaaaaaaagg
    13801 aaagtctcaa agaatagttt atgatgcttt cgcaattgtt gctgaaaaga caggagaaga
    13861 agctttagaa gtatttaata aagcaatgga taatataatg cctgttttag aagtaaaagc
    13921 aagaagagtt ggtggggcaa actaccaagt gccaatcgaa gttagacctg aaagaagaca
    13981 aacattaggt ttaagatggc tagtaaaata tactagagct cgtggagaaa aaggaatggt
    14041 tgaaaagcta gctaaagaga taatggacgc agctaacaac actggagctt cagttaagaa
    14101 aaaagaagat actcataaaa tggcagaagc taataaagca tttgctcatt acagatggta
    14161 ggatatatag atatcttata aaattgagga tatcataagg aggataacca tggctagaaa
    14221 gtttcctttg gaaagaacta ggaatatagg aatcatggct catatagatg caggaaaaac
    14281 tactactaca gaaagaatcc tgttttatac tgggcaaact cataaaatag gagaaactca
    14341 tgaaggagct tctcaaatgg actggatgga gcaagaaaag gaaagaggta taacaataac
    14401 ttctgccgct actactgctt catggaaaga ccatagaata aacataatag atactcctgg
    14461 acacgtggac ttcacagttg aggttgaaag atctctaaga gttcttgacg gttcagttgc
    14521 tgtattctgt gcaaaaggtg gggttgagcc tcaatcagaa aatgtatgga gacaagctga
    14581 aacttatgga gtacctagaa tagcttttgt aaataaaatg gatatattag gtgcagattt
    14641 ctacaatgtt gtaagcatga tgaaatctag attaaattct aatgcagttc ctatgcaatt
    14701 accaataggt aaagaagatt catttatagg tataatagac ttattaaaaa tggatgctgt
    14761 tatatacaaa gacgacctag gggttgaaat ggaagaaaca gatataccag aagatatgaa
    14821 agaattagct gctgagtgga gagaaaaatt agttgaatca gtagctgaaa ctgatgaaga
    14881 attaatgatg aaataccttg aaggtgaaga acttactata gatgaattaa aagttgctat
    14941 aagaaaagct actatagctt gtgaaatgaa cccagttttc tgtggtacag cttatagaaa
    15001 caaaggtgtt cagttagtaa tagatgcagt tttagattac ttaccagcgc caactgatat
    15061 accagctata aaaggtatat tagcagatgg agaagaagct gaaagacatt cttctgatga
    15121 agagccattc tctgcattag cattcaaaat aatgactgac ccattcgttg gaaaactagc
    15181 gttcttcaga gtttattctg gaacattaga atcaggttct tatgttttaa atgcaactaa
    15241 gaataagaga gaaagaattg gtcgtatatt acaaatgcat gctaatacaa gagaagaaat
    15301 aactaaagtg tatgctggag atatagcagc agcagtagga ttaaaagata ctactactgg
    15361 agacacatta tgtgaccctg ctaatccaat aatacttgaa tctatggaat tccctgagcc
    15421 agttatatct gttgctatag aaccaagttc taaagcagct caagaaaaaa tgggtatagc
    15481 tcttcaaaaa ttagctgaag aagacccaac tttcacagtt aagactgacc aagaaacagg
    15541 acaaactata atatctggta tgggtgaatt acacttagag ataatagtag atagattatt
    15601 aagagaattt aaagtagaag ctaaagttgg tgcaccacaa gttgcatata gagagactat
    15661 aactcaacca gttgatgttg aatacaagta ctctaagcaa tcaggtggta gaggacaata
    15721 cggtcacgtt aagattagag ttgctcctca agagccaggt gaaggataca aattcacaaa
    15781 caaaactgtt ggtggatcag taccaaaaga atatgttggt ccagttgaca tgggtataca
    15841 aggagctatg caatctggta tagttgctgg atatccagtt gtagacgttg ctgttgagtt
    15901 atatgatggt tcttaccatg aggttgactc ttctgagatg gcgtttaaga tggctggttc
    15961 aatggcattc aaagatgcta tgaagaaagg taatgcagtt cttttagagc cttacttcaa
    16021 agtagaagtt gttactccag aagattacat gggagatgtt atgggtgact taaactcaag
    16081 aagaggatta atccaaggta tggaagctag aagtggtgca caagttataa atgcattcgt
    16141 tccactttca gaaatgtttg gatactcaac agacctaaga tcttcaactc aaggtcgtgc
    16201 aacttacaca atgatattcg atcactatga gcaagttcca gcttcagttg ctaagaaaat
    16261 agctgaagga aaataattta aaaataatta ataatataaa tataaaaaat aatcagctag
    16321 atgagagata tctctcatct agcacaagat
//
LOCUS       DAEPIN010000078        16222 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.078, whole genome shotgun sequence.
ACCESSION   DAEPIN010000078 DAEPIN010000000
VERSION     DAEPIN010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16222
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.078"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            complement(<1..870)
                     /locus_tag="KR250_003072"
     CDS             complement(<1..870)
                     /locus_tag="KR250_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0068966.1"
                     /translation="MNDELLKKVYTSSFSSASRALKDLTIINTKSLIDDKTQRCVYID
                     ENRLRDELIYYRFYGEKIGNINFLNILLPLILSNTNIQKSEDEVIKLIKKYVIYFKKE
                     ELLFDYLLGSVVYNSIMHNLINNSKIEYVELLQSIKDKIIGFSIELDKSDVVKFQMAR
                     IKAIQLIDKYIDLKSEDYDEESILLNVLNVLYDVYMEDRTVENEGINSIKKSILSILG
                     EDSKLNEDNIDFIFSMSEYVVKLRKYKIGVKAYNKSIDPRSLIRLEEGNTIVDPIFNQ
                     ITVMSKTFNDNILS"
     gene            complement(1124..2824)
                     /locus_tag="KR250_003073"
     CDS             complement(1124..2824)
                     /locus_tag="KR250_003073"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HBH0068967.1"
                     /translation="MQDFKVAISNCLKEKIEDLSKEEIEALIEVPPNKDMGDYAFPCF
                     KLAKVFRKAPNMIASELAESIEPSGEITKVIQLGGYVNFFVNKSQLAETVIKKVLDEK
                     ENYGHSDFGKDKTVIVEYSSPNIAKPFHIGHIRTTVIGNALYKIYDSQGYKTIRINHL
                     GDYGTQFGKLIVAFKKWGEKEVVESNPIPELLKLYVRFHDEAEQHPEMEDEARAWFNK
                     LENGDEEAQELWQWFRNESLKEFNRVYKLLDIEFDSLAGESFYSDKMNRVIELLEEKN
                     LLKESKGARIVDLEEYKMPPALITKNDGSTLYMTRDLAAAIYRKETYDFDKCIYVVGS
                     QQNLHFQQWFKVIELMGYDWAKDLIHVGFGMVALEEGTMSTRKGRVVFLEDALNQAID
                     KTKEIILAKNPNAKNVDEISKQVGVGAVVFQELSNSRIKDYTFSWERTLSFDGETGPY
                     VQYTHARCCAVLRKAEVEVTSDIDYSLLADEDSAEVLRVIESFNKNILLALKKNEPHI
                     VTRFMLDLAQAFNKFYHDNPILVENLEIRKARLALVLATKQTLENSLKLLGMHAPERM
                     "
     gene            complement(3234..3647)
                     /locus_tag="KR250_003074"
     CDS             complement(3234..3647)
                     /locus_tag="KR250_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HBH0068968.1"
                     /translation="MILVSACLIGINCKYSGDNNDNEKVKEYLKGKEFTLVCPEQLGG
                     LSTPRPPAEIVDGKVITKDNKDVTENFVRGANETLKIANLYVCSEAILKEGSPSCGCN
                     LIYDGSFSGKKIPGKGITARLLEEEGIKVKSEKDL"
     gene            complement(3713..6091)
                     /locus_tag="KR250_003075"
     CDS             complement(3713..6091)
                     /locus_tag="KR250_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HBH0068969.1"
                     /translation="MNEKSLRVLEYNKIIDLLKKKASSSLGLKYIENLVPNTDFVEVK
                     SMLEETSEAQSIIIKRGSVGLEGIHDIEDKVKRAYIGASLDPGSLIMIADTLRVARRL
                     RNSLSSSDEEDFNYPIIQSLSNSLYVYKDIEDQIYNAIISEVEISDNASSTLRDIRRR
                     IAQKNQSIRSKLNSIISSTTYQKYLQDAIISLRGDRFVVPVKSEYRSQVAGIVHDQSS
                     SGATLFIEPMTIVEMNNELRQLKLGEQEEIERILSELSAMVGEVSEDLISNQEILGRL
                     DFAFSKGKLSIQMRGIEPTLNEDKYLNIKNGRHPLLDKKKVVANTIYLGRDFHTLVIT
                     GPNTGGKTVTIKTVGLFALMTQSGLHIPADYGSSMCVYDNVFADIGDEQSIEQSLSTF
                     SSHMTNIVSILQNVTADSLVIFDELGAGTDPVEGAALAIAVLEDINSVGAKCIATTHY
                     SELKNYALTKPGVENAAVEFDIETLSPTYKLLIGVPGKSNAFEISRKLGLSDYVISRA
                     KEYINTENIALEDVLQNVEKNRIKAVEDREEAERLKEEIEKLKVEYDEKLEKLVSQRD
                     KMIEKAKSEAFSIIRQAKEEVDIIIKELRSLEQERASKEKNRKIEELRKELTSSMGSL
                     QPTVKSMIVPKVSNKEIKDLKPGEEVKVITLNQNGSVVSVDKKRKEAVVQIGIMKMTL
                     PFKSLQKTRKDVSTNVTKSTRNIIRSKSGSVKNEVDLRGLNLEEAIMEVEKYLDDAYV
                     AGLESVTVIHGIGTGVLKAGLQDILRRNRHVKSQRGGQYGEGGAGVTIVKLK"
     gene            6410..7861
                     /locus_tag="KR250_003076"
     CDS             6410..7861
                     /locus_tag="KR250_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HBH0068970.1"
                     /translation="MGNVLEGLKPESVFKNFEKISQIPRGSGNEKGISDFLLSFGKNL
                     GLETIQDESLNIIIRKPATKGYENCPGVVLQGHMDMVCEKEKNVEHDFLKDPIKLRID
                     GDMIYATGTTLGADNGIAVAMGMAILEDNTLEHPALEVLVTVNEEDGMNGADALDPSL
                     IKGQYILNMDSEEEGYLLVSCAGGKTCVVSLPVEYKEVKGDKQGLLVEVTGLLGGHSG
                     MEIVLQRANANKAIARVLSVLNVDYELASVDGGTKHNAIPREAKCVIAVNKADVESAK
                     KQINDILTAFKHEFTTSDPGMTYSVAETSVDKVLTKDCKEKVVQMSCLTPHGVQSVSL
                     DIEGLVESSTNFAIIETKESTIEFLTSVRSSVMSIRDEIADRIRLLAQALGANYDLIA
                     QYPAWEFKKGSKLEKICSETYEKLTGKVPTVMALHAGLECGLLLDKLPHAEAISIGPD
                     MFDVHTPNEHVSIPSVANVWDYVIEILKSMNQY"
     gene            complement(8065..10578)
                     /locus_tag="KR250_003077"
     CDS             complement(8065..10578)
                     /locus_tag="KR250_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBH0068971.1"
                     /translation="MEDCVVNLKKLLLLIIVITSIFTSCIFSFGINAIGNDNRTIRVA
                     YPIQKGLTEIDEQGNYFGYTYEYLLEIAQYTGWNYEFVQVPGEINESITKLMKMLENG
                     EIDIMGGMLYNEPMQKIYNYAGNNYGVANTVLQALYENTDIDNIMSSQDVKKIRIAVI
                     ENSDTRLKELDEYCKMNLMSPELVYCKTNDEQLEALKSGKADVLLNVSMNPIEGVRTV
                     AKFSSKPFYFITPKGSTNLTQKLNSAINNIEQKDPYFSTTLYEKYFTPENNQMFLSDE
                     ETNYIKETEKLKVGVLLNKPPFQNIDKDTGELRGISIDLLNHICDKTGLEFELVPVKS
                     QEDLENMTKKREIDIVAGMIYDYEYSREHNIAMSRPFLSTQYTMMINNKLNENSLDGK
                     RVALAKGINYYGQFEENIVWYDNLEECIEAVNKGYADYTYGEGYSMQYYMNQPKYKNV
                     RLVPQTYKAKSICFGIIKKDKQELLNIFNKAIISISSEKMQSIVYQNTTYRQDFSLLY
                     IMQQNPIETIFIVASLLICIIGFMAFGLHTRTSMNEKISLELKKHTQLYEMTSEYFFE
                     YDYKQDKLIISMPEKGIETYYGLDKNDKESENGNIENERDKIRKEYNKKFFDVIKSQE
                     NGTTEMYSIMLDDTFHWIRVTSKIIYNDRKIPMYVIGKICNIDSEKEEKNRLLDKAQR
                     DSLTNIYNAAYIRKLTSQNLLNLSTEKNGALLIIDIDYFKSINDTYGHLLGDKVLIDV
                     AKALDESFAKNDIVGRLGGDEFSVYINDIINKEELINKCNDVYNHIHLIKLPNGESLS
                     ISMGVVIAKPGQEYDELYQIADNALYDSKRQGRNRFKIV"
     gene            complement(10801..11622)
                     /locus_tag="KR250_003078"
     CDS             complement(10801..11622)
                     /locus_tag="KR250_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBH0068972.1"
                     /translation="MIDINLEKCVGCGMCESDCLVNAIKVKDDKAKVKNILCINCGHC
                     MAICPTDAIEMQGFDKNEVIEYNRESFELEPEKLLNFIKFRRSIRQYKDIEVEEEKIK
                     NIVEAGRYTPTGGNRQPIRYILVKEKLKEVKELAIQGLYNLALDTDDNDPVRSIYKNT
                     FKKMYKRYKENGNDSLFYDAPLLMVVVGDMSLGGSAYVDGGLAASNMELMAYSQGLGI
                     CYNGFFVMASNVEPKIKELLGMSENEAVITSFILGYPDVKYKRTVNRNTAKFEVR"
     gene            complement(12140..12616)
                     /locus_tag="KR250_003079"
     CDS             complement(12140..12616)
                     /locus_tag="KR250_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HBH0068973.1"
                     /translation="MKLTTICYIEKDDKTLMLYRNKKKDDIHEGKYVGVGGKFEQGET
                     PEECVIREVKEETGLTLKSLSYKGLITFPKFKDEEDWYMFLYFSDEFEGELSEKDLND
                     CKEGNLIWVDNDKIFDLNMWEGDRLFLNWAKTGNIFSAKIVYDNGKLKDYNVSFLD"
     gene            complement(12651..13229)
                     /locus_tag="KR250_003080"
     CDS             complement(12651..13229)
                     /locus_tag="KR250_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBH0068974.1"
                     /translation="MREEALEILFKYLETDRMRKHCYAVEAVMRELAKRLEPEKEEEW
                     AVAGLLHDLDSDIVGRKPGEICEGHATTTVELLKKENFGNEEMYRAILGHHDGMGVTR
                     TSLMEKAIYAADPITGFITAIALVYPDKKLTSVKTKSVIKRMKETRFASNVNRDAMRS
                     IEDIGIPFDEFAELSLNAMKNISDVLESAENN"
     gene            complement(13333..15933)
                     /locus_tag="KR250_003081"
     CDS             complement(13333..15933)
                     /locus_tag="KR250_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBH0068975.1"
                     /translation="MKKIELLAPVGSFDSLKAAVQNGANAVYLGGKDFSARASANNFD
                     RNELIEAVKYSHIRGVQVFVTVNTLIKQYEIEDFVEYVKFLYDIDIDALIVQDIGMAR
                     LIKKLLPDFELHASTQMVAHSLEDVKYLQSVGFDRVVLARELNVDEIKWICENTTVDI
                     EVFVHGALCVCYSGQCLMSSMIGNRSGNRGRCAQPCRQKYELIDINTGEVVKSNGDYL
                     LSPRDLSTIEDLDKIIEAGVLSLKIEGRMKKPEYVATVVSGYREAINEFVDKKKISIS
                     EEIINNLYTIFNRKFTKGYILGEVGKDIMNSNRPNNQGLYIGKVLDYNRKNKRLRVML
                     ENTLKKGDGINLGGGTIGRIIKDGEITTIAHKGDLIELDFVGEAKRNQLIYKTSDSEL
                     MDRVQATFQQDKEFIKTNIRAKVSIKLGKKPELFISDFCGNKSVVEGDKIVEKAIKVA
                     LSKEKIETQIQKLGNTPYKLKEIEIDLDEGVSMPISVLNQMRRDCIELLDEERIKIKD
                     RKFKKNRLKYQPNRVNKQKKLDTAPKIRVKVKNLEQLEAVLNYNVDLIYYEDINTLSK
                     ALQMVSSISIDSNISIDSSISMDLDTSSSLGTPKELMGSNTNNIDTLSNTSKIDTLNK
                     NHSNTAVTDKKLIYSAPRIVRNKEYKQFEILDNIKGINKVQIGNWGSMEYFKKKDLNI
                     DCYLNVFNSESINHFKEEGASTVCLSQELNLNEIKQTLKYTDVEIEAVIYGYAPMMVT
                     EYCPMGVVVRDCKKDKRVAKCKESSYALRDFTDASYRITQDIFCRSTIYNSKVTCMLD
                     NLYELEEIGIDVFRIDFTVEDSEMVKKVIEAHIEVLSNNYKLGKKATDLYKKLDEIGT
                     VAGHYYKGVE"
BASE COUNT         4992 a         2907 c         1791 g         6532 t
ORIGIN      
        1 gcttaatata ttatcattaa aggttttcga cataactgtt atttgattaa atatagggtc
       61 tactatagta ttaccttctt caagtcttat aagacttctt gggtctatgc ttttattata
      121 tgccttaact ccaattttat attttctcaa tttaacaaca tattcggaca tagaaaaaat
      181 aaaatctata ttatcttcat ttaatttact atcttcaccc aaaatggata atatagattt
      241 ttttatgcta ttaattcctt cattttctac agttctatct tccatatata catcatatag
      301 aacatttaaa acatttaata aaatactttc ttcatcataa tcttcagatt tcaaatcaat
      361 gtacttatca attaattgta ttgctttaat ccttgccatc tgaaacttta caacatctga
      421 tttgtctaat tctatagaaa atcctattat cttgtcttta atactctgta gtaattcgac
      481 atactctatt ttactattat ttataagatt gtgcataatg gaattataca ccacagaacc
      541 taataaataa tcaaataaaa gttcttcttt tttaaaataa ataacatact tttttattag
      601 tttaataacc tcatcttcac ttttttgaat attagtattt gataaaatta gtggtaataa
      661 aatatttaaa aaattgatat ttcctatttt ttctccataa aatctatagt aaataagttc
      721 atctctcagt ctattttcgt ctatatacac acatctttgc gttttatcat ctattaagga
      781 ttttgtattt attatagtta aatctttaag agccctactt gcactactaa aagaagatgt
      841 gtagactttt tttagtaatt cgtcattcat aaatatatcc tttctgaaaa attgaatgta
      901 aataaattgt tattaaaaaa aaagagacag cccgtttaag aagctgtcat atataggggg
      961 atagtagtgt acaaaaatca ttgtaatcta ctatctatat attattatac gatactactt
     1021 ttttatatgt aaagcatcag ttttcgacat ccctatattt tacagttata caaattgcgt
     1081 actaagttca attttattct ttttatttca acattttatc atgctacatt ctttcaggag
     1141 cgtgcattcc caacaatttc agagaatttt ctaaagtttg ttttgttgct aaaactaatg
     1201 caagtcttgc ttttcttatt tccaaatttt ctactaaaat tggattgtca tgataaaatt
     1261 tattaaacgc ttgagctaaa tctagcataa atctagtcac tatatgtggc tcatttttct
     1321 ttagagcaag taaaatattc ttattgaatg attctataac tctaagaact tctgcactat
     1381 cttcatcagc taatagacta taatctatat cagaagtcac ttctacttct gcttttctta
     1441 aaactgcaca gcatctagca tgtgtatact gaacatatgg tccagtctca ccatcaaaac
     1501 taagcgttct ttcccaagag aaggtatagt ctttaattct actattagat aactcttgga
     1561 atactactgc cccaacacct acttgcttag atatttcatc cacattttta gcatttgggt
     1621 ttttagctag tataatttcc ttagttttat ctatagcttg atttaaagca tcttctaaaa
     1681 atactactct tccttttcta gtcgacatag taccttcttc aagtgcaacc ataccaaatc
     1741 caacatgaat taaatctttt gcccaatcat atcccataag ctcaattacc ttaaaccatt
     1801 gttggaaatg taagttttgt tgagaaccaa ctacatatat acacttgtca aaatcataag
     1861 tttcttttct atatattgca gcagctaaat ctcttgtcat atataacgtt gaaccatcat
     1921 tcttagtaat aagtgctggt ggcattttat actcttctaa atcaacaatt cttgctcctt
     1981 tagactcttt taaaagattt ttctcttcta atagttcgat aactctgttc attttatcag
     2041 aataaaaact ctctcctgct aaagaatcaa attctatatc taaaagttta taaactctgt
     2101 taaactcttt taaactctca tttctaaacc attgccataa ttcttgtgct tcttcgtcac
     2161 cattttctaa tttattaaac catgctcttg cttcatcttc catttctggg tgttgttcag
     2221 cttcatcatg gaatcttacg tataatttta gtaattctgg aattggatta gactctacta
     2281 cttctttttc tccccacttt ttaaaggcca ctattaactt accaaactga gttccatagt
     2341 cacctaaatg atttattctt atagtcttgt atccttgcga atcatatatc ttatataaag
     2401 cattacctat aactgtagtt cttatgtgcc ctatatggaa aggttttgct atatttggag
     2461 aagaatattc aactattaca gttttatctt ttccaaaatc actatgtccg taattttctt
     2521 tttcatctaa aactttttta attacagttt ctgctaattg agatttattt acaaagaaat
     2581 ttacatatcc gcctaattgt attacttttg ttatctctcc acttggttct atactttccg
     2641 ccaattcact tgctatcatg tttggagctt ttctaaacac tttggctaat ttaaagcatg
     2701 gaaaagcata gtcccccata tccttatttg gtggaacttc tataagtgcc tctatctctt
     2761 ctttcgataa atcttctatt ttttctttaa ggcaatttga tattgccacc ttaaaatctt
     2821 gcattctgtt gctcctctct acattgttcc tggtttatat tgtgcttatc tctttatgtt
     2881 aaatcaaatt tcgtgttttt tcaagtattt cattacagtg taaaacactt tagtaattat
     2941 gttttctgta tatattaaaa atactataat ttcagttttt aatcacacta ttattgattt
     3001 tttataagac ttttttctat tttattgttt cttgtactat ccagttgact ttatatactt
     3061 tttttaatct tttatctgtt ttcatacatc aatgtcagct ttatatacat ttttttaatc
     3121 ttttatctat tttcatacat caatgtcagc tttatatact ttttttactt ttttatctat
     3181 ttttatacat caatattagc tttatatact ttttttactc ttttatctat tttctataaa
     3241 tctttttcac tcttaacttt tattccttct tcttctaata gccttgctgt tatacctttc
     3301 cctggtatct ttttacctga aaaactacca tcatatatta gattacatcc acaagagggg
     3361 ctaccctctt ttagtattgc ttcgctacat acatataaat ttgctatttt tagagtttca
     3421 ttagcccctc ttacaaagtt ttctgttaca tccttattat ctttagtaat tacttttcca
     3481 tcaactatct cagcaggtgg tcttggtgta gatagaccac ctaactgctc tgggcataca
     3541 agtgtaaatt ctttgccttt taaatattcc ttgacttttt cattatcatt attatcgcca
     3601 ctatatttac aatttattcc tattaaacat gcagatacca atatcataaa aatgtgctcc
     3661 tatacaaatt atatatattc tcatacaaat tactaatgta ttcttataca aattatttta
     3721 atttaacaat tgttactcct gctccacctt ctccatattg accacctctt tgtgatttta
     3781 catgtctatt tcttcttaaa atgtcttgta gccctgcttt taatactcct gtacctattc
     3841 catgtattac tgttacagac tctaaacctg ccacatatgc atcatcaaga tacttttcta
     3901 cttccattat agcttcttct aggtttaacc ctctaaggtc aacttcattt tttacactac
     3961 cagattttga ccttataatg tttctcgttg atttagttac atttgttgaa acatctttgc
     4021 gtgttttttg taaagattta aaaggtagag tcatcttcat tataccaatt tgcactactg
     4081 cttcttttct ctttttatct actgaaacta cactaccatt ttgatttaaa gttattacct
     4141 taacttcttc acctggcttt aagtctttta tctccttatt tgataccttt ggaactatca
     4201 tactttttac tgttggctga agacttccca tagagctagt caattctttt ctaagttctt
     4261 ctatcttacg attcttttct tttgaagctc tttcttgttc aagacttcta agttctttta
     4321 ttataatatc tacttcttct ttagcttgtc ttataattga aaatgcttct gatttagctt
     4381 tttcaatcat cttatctctt tgactgacca atttttcaag tttttcgtca tactctactt
     4441 tcagtttttc aatctcttct tttaatcttt cagcttcttc tctatcttct actgctttga
     4501 ttctattctt ttcaacattc tgtaacacat cttctaaagc tatgttttct gtatttatat
     4561 attctttagc acgtgaaatc acataatcac ttagcccaag ttttctagaa atctcaaacg
     4621 catttgattt tccgggaaca cctataagta acttatatgt tgggcttaaa gtttctatat
     4681 caaattcaac tgctgcgttt tctacacctg gtttagtaag agcataattt ttaagctcac
     4741 tatagtgagt ggtagctata catttagctc ctacagaatt tatatcttct aatactgcta
     4801 ttgcaagggc agctccttca acagggtctg ttcctgctcc taactcgtca aatataacta
     4861 aagagtctgc tgttacgttt tgtaatatag atactatatt cgtcatatga gatgaaaatg
     4921 ttgatagact ctgttcaata ctctgctcat ctcctatatc tgcaaataca ttatcatata
     4981 cacacatact acttccataa tcagcaggta tatgaagtcc actttgtgtc ataagtgcaa
     5041 atagacctac agtttttatt gtaacagtct taccacctgt gtttggacct gttatcacca
     5101 atgtatggaa gtctcttcct aaataaatgg tatttgctac taccttcttt ttatccagca
     5161 gtggatgtct tccatttttt atgtttaaat atttatcctc atttaaagtt ggttctattc
     5221 ctctcatctg tatagaaagt tttccctttg aaaatgcaaa gtccaatctt ccaagtatct
     5281 cttgattaga gattaaatct tcgctcactt ctccaaccat agcagaaagc tctgataaaa
     5341 ttctttctat ttcttcttgc tcacctagtt ttaactgtct caattcattg ttcatctcta
     5401 ctatagtcat tggctctata aaaagtgtcg caccagaaga tgactggtca tgcactatac
     5461 cagctacttg cgacctatat tcagatttta caggtactac aaatctatca cctcttagag
     5521 aaattattgc atcttgtaag tacttttgat aagtagttga acttattatt gaatttaatt
     5581 ttgacctaat ggattgattt ttttgggcta ttcttcttct gatatctcta agtgtacttg
     5641 aagcattgtc agatatttct acttcactaa ttatagcatt gtatatctgg tcttctatgt
     5701 ccttgtatac atatagagaa tttgatagag attgtattat tggataatta aaatcttctt
     5761 catcactact agataagcta tttcttaatc ttctagccac tcttaaggta tctgctatca
     5821 taatcagact tcctgggtca agagaagcac ctatataagc tctctttacc ttatcttcta
     5881 tatcatgaat accttccaac cctactgaac ctctctttat aataattgat tgagcttcac
     5941 tagtttcttc tagcatagat ttcacttcta caaaatcagt atttggtact aaattttcta
     6001 tatattttag acctaaagaa gatgaggctt tcttctttaa caaatctatt attttattgt
     6061 attctagaac tcttaacgat ttctcattca ttttctatat tcaccatcct cgatatttta
     6121 acttttttat ttaattttaa aaatgtatgt tttcaagcat ttccaataaa aactgcaagt
     6181 tttatttctt tttcatttct aaaattatta tattttttct cttttagata tattttacac
     6241 atattttgta ttatactatg tatgtagcta tctatatatt atacataaac atgctttatt
     6301 tgtaatataa tctgttaaaa aatcttcttt aatattttta aaggagttta ttttagctta
     6361 taaaaaaaaa tagctactta tatagataaa ttaaggagga acatgagtca tgggaaacgt
     6421 attagaagga ttaaaaccag aaagtgtttt taaaaacttt gagaaaatat ctcaaattcc
     6481 tagaggttct ggaaatgaaa aaggcataag tgatttttta cttagctttg gtaaaaattt
     6541 aggtcttgaa actattcagg atgaaagctt aaacataata ataagaaagc ctgctacaaa
     6601 aggatatgaa aattgtccag gtgtagttct tcaaggacat atggatatgg tttgcgaaaa
     6661 agaaaagaat gttgaacatg attttcttaa agaccctata aaattaagaa ttgatggtga
     6721 catgatatat gctactggaa ctactctagg agctgacaat ggtatagctg ttgctatggg
     6781 tatggctata ttagaagata atactttaga gcatcctgca ctagaagttt tagttactgt
     6841 taatgaagaa gatggtatga atggtgcaga tgcactagac cctagcttaa ttaaaggtca
     6901 atatatccta aatatggact ccgaggagga aggatactta ttagttagct gtgctggtgg
     6961 taaaacttgt gttgttagtt tacctgttga atacaaggaa gttaaaggtg acaaacaagg
     7021 attattagtt gaggttactg gactattagg tggacactct ggtatggaaa ttgttttaca
     7081 aagagcaaat gctaacaaag caatagctag agtattaagt gtattgaatg ttgattatga
     7141 attagcttct gttgatggtg gtactaagca caatgctata cctcgtgaag ctaaatgtgt
     7201 aatagctgtt aataaagctg atgttgaatc tgctaaaaaa caaataaatg acattttaac
     7261 tgcatttaaa catgagttta ctacttctga ccctggcatg acttactcag tagctgaaac
     7321 ttctgtagac aaagttctta ctaaagattg taaagaaaaa gttgttcaaa tgtcttgctt
     7381 aactcctcat ggagttcaat cagtaagtct tgatatagaa ggattagttg aaagttctac
     7441 taacttcgct ataatcgaaa ctaaagaatc tactattgaa tttttaactt ctgttagaag
     7501 ttctgttatg tctattagag atgaaatcgc tgatagaata agacttttgg ctcaagcttt
     7561 aggtgctaat tacgacctta tagctcaata cccagcttgg gaatttaaaa aaggctcaaa
     7621 attagaaaaa atatgcagtg aaacttatga gaaattaact ggtaaagttc ctactgttat
     7681 ggctctacat gctggtcttg agtgtggatt attacttgat aaacttcctc atgctgaagc
     7741 tatctctata ggaccagata tgtttgatgt tcatactcca aatgaacatg ttagtatccc
     7801 ttctgtagct aatgtttggg attatgttat tgaaatatta aaatctatga accaatatta
     7861 aatttaagtt ttgattctaa gtagatttta attttaagta atctatataa aattaactct
     7921 gtaaattaat actacggtta gtataatatt tactttcaat attaacttat agaaataaag
     7981 cctaatgata gataataaca tgtatcatta ggctttattg taatttaaat attattaagc
     8041 tttattctgt tttaaaatta attcttatac tattttaaat ctatttcttc cttgtctctt
     8101 agaatcataa agagcattat ctgctatttg atacagttca tcatactctt gaccaggctt
     8161 tgctataact acccccatac taatagataa actttctcca tttggcaact taatgagatg
     8221 aatatgattg tatacatcat tacatttatt aataagttct tccttattta ttatatcatt
     8281 gatataaaca ctaaattcat ctccacccaa acgacctact atatcatttt ttgcaaaact
     8341 ttcatcaagt gcctttgcaa catcaataag tactttatct cctaacaagt gaccataagt
     8401 atcattaatc gatttaaaat aatcaatatc tataatcaat aaagcaccat ttttttcagt
     8461 tgataaattc aacaaatttt gtgaagttaa ttttctaata tatgctgcat tataaatatt
     8521 ggttaaacta tccctttgag ccttatctaa caatctattc ttttcctctt tttcagaatc
     8581 aatattacat attttcccga ttacatacat tggtattttt ctatcattat aaattatttt
     8641 tgaagtaact ctaatccaat gaaaagtatc atcaagcata atagaataca tttcagttgt
     8701 tccattttct tgtgatttta ttacatcaaa aaacttttta ttatattctt tccttatttt
     8761 atctctttca ttttctatat tgccattttc actttcctta tcatttttat ccaaaccata
     8821 ataagtctca ataccctttt ctggcataga aattatcaat ttatcttgct tataatcata
     8881 ctcaaaaaag tattcacttg tcatttcata caactgagta tgttttttga gttctaaaga
     8941 tatcttctcg ttcatacttg tacgagtatg taaaccaaat gccataaatc caataataca
     9001 aattaataaa ctggctacaa taaaaatagt ctcaattgga ttctgttgca taatataaag
     9061 caacgagaaa tcttgtctat atgtagtatt ttggtagaca attgattgca ttttttctga
     9121 tgaaatactt ataatagctt tattaaatat attcaataat tcttgtttat cttttttaat
     9181 tataccaaaa caaatactct ttgctttata ggtttgagga actagacgca catttttata
     9241 cttaggttga ttcatatagt actgcataga ataaccttcg ccataagtgt aatctgcata
     9301 gcctttatta acagcttcta tacactcttc taaattatca taccaaacta tattctcttc
     9361 aaactgacca taataattaa tacctttggc tagagctaca cgttttccat ctaaactatt
     9421 ctcatttaat ttattattaa tcatcatagt atattgagta gaaagaaaag gccgagacat
     9481 agctatatta tgttcacgag agtattcata atcataaatc attcctgcta caatatcaat
     9541 ttcacgcttt ttagtcatgt tttctaaatc ttcttgagat tttacaggca caagttcaaa
     9601 ttctaagcct gttttatcac atatatgatt taacaaatca attgatatac ctcttaattc
     9661 acctgtatct ttatctatat tttgaaatgg tggtttgttt aaaagtactc ctacttttaa
     9721 tttttctgtc tctttaatgt aattagtttc ttcatcagat aaaaacattt gattattctc
     9781 tggtgtaaag tatttttcat ataaagtagt tgaaaaatat gggtctttct gttcaatatt
     9841 attaattgct gagttaagct tttgagttaa atttgtacta cctttaggag taatgaaata
     9901 aaatggtttt gatgaaaatt ttgctacagt acgtacacct tctattgggt tcatactcac
     9961 attaagaagt acatctgctt taccactctt tagtgcttct agttgctcat catttgtttt
    10021 acaatatacc aattcaggtg acataagatt cattttacaa tactcatcta attctttaag
    10081 tctagtatca gaattttcta ttacagcaat acggattttt ttcacatctt gtgaactcat
    10141 tatgttatca atatctgtat tttcatataa tgcttgtagt actgtatttg ctactccata
    10201 attattccct gcataattat atattttttg cattggttca ttgtataaca tacctcccat
    10261 tatatcaatt tcaccatttt ctaacatctt cattaatttt gtaatagatt catttatctc
    10321 tccaggaact tgaacaaatt cataattcca tccagtatac tgtgcaattt ctagtaaata
    10381 ttcatatgta tacccaaaat agttaccttg ctcatctatt tcagttaaac ctttttgaat
    10441 aggataagca acacgaatgg tacgattatc attacctata gcgttgatac caaaagaaaa
    10501 tatgcaagaa gtgaatatag atgtaattac aataattaat aaaagtaatt tttttaaatt
    10561 cactacacaa tcctccatat agttaagatt tacagtataa attactgcat aaacttgtag
    10621 acatgtaaaa atagattagt atataaaagg atatataaat ctacttagaa atttaaatat
    10681 ccttaatata tacaatatat ttaaattata actcatttat atgtctatta caacaaattt
    10741 accctgctaa atacctactc tatatcccat aattttacaa gttgtagtga agtttaagtt
    10801 ctatctcacc tcaaatttag cagtgtttct atttacagtt cttttatatt tcacatctgg
    10861 ataacccaat ataaatgaag tgataactgc ttcgttctcg ctcattccta acagttcttt
    10921 tatctttggc tctacatttg atgccataac aaaaaatcca ttataacaaa ttcctaatcc
    10981 ttgagaatag gccataagtt ccatattaga tgcagccaat cctccatcta cataagcact
    11041 tccacctaga gacatatcac ctacaactac cataagtagt ggggcatcat aaaataaaga
    11101 atcatttccg ttctctttgt atcttttgta cattttctta aatgtatttt tatagataga
    11161 acgtacaggg tcattgtcat cagtatctaa tgccaaatta taaagacctt gtattgccag
    11221 ttctttcact tcttttagct tttcttttac taatatgtat cttataggct gtctattacc
    11281 acctgttggt gtgtatctac cagcttccac tatgtttttt attttttctt cttctacctc
    11341 tatatcttta tattgtctta tacttcttct aaatttaata aaattcaata acttttcggg
    11401 ttctaattca aaactttcac gattgtattc tattacttca tttttatcaa atccttgcat
    11461 ttctattgca tctgttgggc aaattgccat acagtgacca cagttaatac agagaatatt
    11521 tttaaccttt gctttatcat ccttaacttt tattgcattt acaagacagt ctgattcaca
    11581 catcccacag cctacacatt tttctaaatt tatgtctatc attccaaatt attcctcctt
    11641 ttaaaaaatt tactttagta taaaacaaat agttgtctaa gtcaattttt atttttttaa
    11701 caatatacta gccttaacta cactttaatg gagaaaaata tacataaatt ggaacttaaa
    11761 ttagaactta ttcaaagtaa aagataaaag aagtagatgt ctaaaagtga actttttagt
    11821 taattagata ttctttattg tatgaaatca agtggatatt tatcatttca acaataaaaa
    11881 aggcttacct ctattttgga tatgtccctg tcaagtagac agactaaaaa aaggctatct
    11941 ttatgcactg agatagcccc ttttcccaac tactattgtt tatcttaata ttagtcaata
    12001 aatttaattt gctataacct gtaagttaca ttttaatttg atgtaacttt aaattacatt
    12061 tttaagtaat atctttaaat tatattctta aaatatatca ttaaactaca ttttaattaa
    12121 tctaaaaact atatcttaat taatctaaga aacttacatt ataatctttt agtttcccat
    12181 tatcatatac aatctttgca ctaaagatat ttccagtctt agcccaattt aagaacagcc
    12241 tgtcaccttc ccacatattt aaatcgaata ttttatcatt atcaacccaa attaaatttc
    12301 cttctttaca atcatttaaa tctttttcag ataattcacc ttcaaactca tctgagaaat
    12361 acaaaaacat ataccaatct tcttcatctt taaattttgg aaaagttatc aaccctttat
    12421 atgacaatga tttaagagta agaccagtct cttcttttac ttctctaatt acacattctt
    12481 ctggagtctc tccttgctca aactttcctc caactcctac atacttacct tcatgtatat
    12541 catctttctt tttatttctg tacaacatca aagtcttgtc atctttttct atataacaaa
    12601 tagttgtaag tttcatgtat ctcgccttgc tttctctttt atattctcaa ctaattattt
    12661 tcagcacttt caagcacatc acttatattc ttcattgcat taagagaaag ctctgcaaat
    12721 tcatcaaatg gaatccctat atcttctata cttctcattg catctctatt tacatttgaa
    12781 gcaaatctcg tttctttcat tcttttaata acagacttag tctttacact ggtaagtttt
    12841 ttatctggat aaactaaagc tatagcagtt ataaacccag ttatagggtc tgctgcatat
    12901 attgcttttt ccattaggct agttcttgta actcccattc catcatgatg accaagtatt
    12961 gctctataca tttcttcgtt accgaagttt tcttttttaa gcagttctac tgttgttgta
    13021 gcatgtcctt cacatatttc tcctggcttt cttccaacta tatctgaatc caaatcatgt
    13081 aaaagacctg ccactgccca ctcttcttct ttttctggct ctagtctttt ggctaattct
    13141 ctcataacag cttcaacagc ataacaatgt tttctcattc tatcagtttc taaatactta
    13201 aataaaattt ctaaagcttc ttctctcatt ttcaccaatc cccctgtttt ttcacatata
    13261 tgatattttt atatattaat acacataaat tcaagctttt atatatattc atgtatattt
    13321 ttcatgttat atctattcta cacctttata gtagtgtcct gcaactgttc caatttcatc
    13381 taatttctta tacaagtctg tagccttttt tccaagttta taattattac tcagtacctc
    13441 aatgtgagct tcaataacct ttttaaccat ctcactatct tcaactgtaa aatctatcct
    13501 aaatacatct attccaattt cctctaactc atatagattg tccaacatac aagtaacctt
    13561 cgaattgtaa attgtacttc tacagaatat atcttgtgtt atcctataac ttgcatccgt
    13621 aaagtctctt aaagcataac tactttcctt acatttagca actctcttgt cctttttaca
    13681 atctctaaca actactccca taggacagta ttctgtaacc atcataggag catatccata
    13741 tattactgct tcaatttcta catctgtata ttttaaagtt tgtttaattt catttaagtt
    13801 tagttcttgt gacaaacaaa ctgtacttgc tccctcttct ttgaagtgat ttatactttc
    13861 actattgaat acatttaagt agcaatctat atttaagtct tttttcttaa agtattccat
    13921 agagccccag ttaccaattt gaaccttatt aattcctttt atattatcca atatctcaaa
    13981 ttgcttgtac tctttatttc taacaatcct tggtgctgaa tagattaatt ttttgtcagt
    14041 gactgctgta ttagagtggt tcttattcaa agtatctatt ttacttgtat ttgacaaagt
    14101 atctatgtta tttgtatttg aacccattag ttctttagga gtacctaaag acgaagaagt
    14161 atctaaatcc atagaaatac ttgaatctat agaaatattt gaatctatag agatacttga
    14221 gaccatttgt aaagcttttg ataatgtatt tatatcttca taatagatta aatctacatt
    14281 ataatttaat actgcttcta attgctccaa gttcttaact ttaactctaa tcttaggagc
    14341 tgtatcaagt tttttctgtt tatttactct atttggctgg tattttaatc tattcttctt
    14401 aaattttcta tcttttatct ttattctttc ttcatctaaa agctcaatac aatcccttct
    14461 catttgattt aacacactta taggcatact tacaccttca tccaggtcaa tttctatctc
    14521 ttttaactta tatggtgtat ttccaagctt ttgaatttgt gtttctattt tttctttact
    14581 taaagccacc tttatagctt tttcaactat tttgtctcct tctaccaccg atttatttcc
    14641 acaaaaatca cttataaaaa gctctggctt tttacctagt ttaatactca cttttgcacg
    14701 tatattagtt tttatgaact ctttatcttg ctgaaatgta gcttgtactc tatccattaa
    14761 ttcgctgtca gaagtcttat atataagctg atttctcttt gcttctccaa caaaatctaa
    14821 ttctatcaaa tcccctttat gtgctattgt agttatctct ccatccttta ttattcttcc
    14881 aatagtacct ccacctaagt taattccatc acctttctta agcgtatttt ctagcattac
    14941 tctaagtctt ttgttctttc tattataatc taaaactttt ccaatatata gcccttgatt
    15001 attaggtcta ttagagttca ttatatcctt accaacttca cctaatatat atccttttgt
    15061 aaactttcta ttaaatatgg tatacaaatt atttattatt tcctcagaaa tacttatttt
    15121 tttcttatct acaaattcat taatcgcttc tctatatcct gaaaccacag tagctacata
    15181 ctctggcttt ttcattctac cttcaatttt taaagataat acacctgctt caattatttt
    15241 atccaaatct tctatagtac ttaaatctct aggactcaac aaataatctc cattactttt
    15301 tactacttct cctgtattta tatctatcaa ctcatacttt tgtctacatg gctgtgcaca
    15361 cctacctcta tttccagacc tattgcctat catactactc ataaggcatt gacccgagta
    15421 acatacacat aaagctccat gtacaaaaac ttctatatct acagttgtat tttcacaaat
    15481 ccattttatc tcatcaacat ttaattctct tgctaaaaca actctatcaa accctacact
    15541 ctgaaggtat tttacatctt ctaaggaatg tgctaccatt tgagtgcttg catgtagttc
    15601 aaagtcaggt aatagttttt ttataagtct tgccattcct atatcctgta ctattaaagc
    15661 atcaatatct atatcgtata aaaattttac atactctaca aaatcttcta tttcatactg
    15721 ttttatcaat gtatttacag taacaaagac ttgcactccc cttatatgag agtatttaac
    15781 agcctcaatc agctcatttc tatcaaagtt atttgcagat gctctagcac taaagtcttt
    15841 accacctaaa tacacagcat ttgctccgtt ttgaactgct gcttttaatg aatcaaaaga
    15901 gcctactggt gcaagtaatt ctattttttt catatttttc tcctctattt atacttgtta
    15961 ggttaatata attatatcta taacttaaat atttgtatta aatttatata tttcttacta
    16021 tattttaaat tcttcaatca atttatatat tagttattta tatttggttc atttgagtta
    16081 acatatacat tatttactat atttagtata tttctattaa cttatatatt gattactgta
    16141 ttttaaattc ttcaattaat ttatatactg cttactaaat ttaaaacatt ttaattaatt
    16201 tgtacactgt ttactatact tg
//
LOCUS       DAEPIN010000079        15501 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.079, whole genome shotgun sequence.
ACCESSION   DAEPIN010000079 DAEPIN010000000
VERSION     DAEPIN010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15501
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.079"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            49..225
                     /locus_tag="KR250_003082"
     CDS             49..225
                     /locus_tag="KR250_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0068976.1"
                     /translation="MFRDKMDRCTHMLTAYIGSSYDYCDFIDTQLDDFILEYGENVVE
                     SCLHQVMVLVSKYN"
     gene            635..1273
                     /locus_tag="KR250_003083"
     CDS             635..1273
                     /locus_tag="KR250_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0068977.1"
                     /translation="MSNQRKDFDITNDMFQESIPITTKIILEDMPSNDELNHVFSKGC
                     ERKMKKRKIVLITLLLIGVLLLGSILYNLFLGKTANISMLKESWNFDIPIPNKEIEVF
                     DTQDSINGDGQSYFIQGFSEKNFKKVFNLKGGIVVSKDNINEIEKYIDKFKRDSVNIN
                     KSNKNKIEEDFKKYKLEVKKDDKYIYKRNYENYVVLIIKKDEQKLYSLIWNQ"
     gene            complement(1762..3522)
                     /locus_tag="KR250_003084"
     CDS             complement(1762..3522)
                     /locus_tag="KR250_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBH0068978.1"
                     /translation="MQEVEVIKAKNVLARQARGKAIERLRVAAYCRVSTDSEDQLNSY
                     KSQVQYYTDMIKKNKEWVLADIYADEAITGTQVTKREDFQRMINDCMNGEIDMVFTKS
                     ISRFARNTLDTLKYVRMLKERNIAVYFEDEKINTLTMDGELLLVVLSSVAQQEVENIS
                     ANVKKGLKMKMKRGELVGFQGCIGYDYHPEDKTITVNQEEAEIVRYIFNRYVDGAGGS
                     VIGQELESLGYKTKYGSSTWAPSTVIGIIKNEKYKGDILLGKTFTVDPISKRRLENMG
                     EEDKFYMKDHHEPIISEEVFEKAQEILNRRNKNRTTVGSGKREKYSRKYAFSCMLECG
                     FCGGTLTRRNWHSGSKYSKVIWQCVTATKKGKKFCKHSKGIPETAIEEAFVESYRLLC
                     DDNKDVLEEFLQRMDDTLSSSVVSKQLAKAEKEIDALEKKKSKLVDMRLEEIIDKETY
                     ESKYADLVSKQEQLVEERQKLQETSDNEKDIKKRLKEFKKTLEQNEVLDKFDRYVFES
                     IVEKVIVGGLDENGNVDPAQLTFVYKTGLKNSVDGAKFKTQRKNARGRHRTNELCSHD
                     SNEVDKMCSDSSNDTCGNCS"
     gene            complement(3585..3728)
                     /locus_tag="KR250_003085"
     CDS             complement(3585..3728)
                     /locus_tag="KR250_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBH0068979.1"
                     /translation="MNNIIRYSMQIAMLKQLLARKLISEKEYTLVKQRLMKDYKVLSD
                     ITA"
     gene            complement(4047..4286)
                     /locus_tag="KR250_003086"
     CDS             complement(4047..4286)
                     /locus_tag="KR250_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002592280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH0068980.1"
                     /translation="MMKSTKKCPSFSTISSAADGNEVAIEKILNHYDAYISKASLRPF
                     YDEHGNMYIVLDMELKGRIRAALIKVILGFEVKVK"
     gene            complement(4291..4707)
                     /locus_tag="KR250_003087"
     CDS             complement(4291..4707)
                     /locus_tag="KR250_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002592281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBH0068981.1"
                     /translation="MKPSSFEGAIRLQFDCLARKVVGRTVKNYDRELGRRARRETPFC
                     DLSEMELNQIGAMDEYCVEFTSFDVFGSEVHIYDERLCEAIKELTESRRNILLMSYFL
                     EMTDAEIAEVMEMERFSVCRNRLHTLKLIKDMYEEE"
     gene            complement(4704..5003)
                     /locus_tag="KR250_003088"
     CDS             complement(4704..5003)
                     /locus_tag="KR250_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBH0068982.1"
                     /translation="MKHLHIQQLSTIGSSLKKHLNSTEHNAPICGFLQHITGIICLAQ
                     DQYVCIALVLFYCIKLFQKIFAVGYQIPPFMVSVSAKRGRSLFALATFAGKGVRL"
     gene            complement(6054..6221)
                     /locus_tag="KR250_003089"
     CDS             complement(6054..6221)
                     /locus_tag="KR250_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002587909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0068983.1"
                     /translation="MNWKELWMTLFGTTEWLGLNIGFWVAMAVVLLIVIVMNVVFWSM
                     KPKKDTKESYK"
     gene            complement(6234..8999)
                     /gene="mgtA"
                     /locus_tag="KR250_003090"
     CDS             complement(6234..8999)
                     /gene="mgtA"
                     /locus_tag="KR250_003090"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002571344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HBH0068984.1"
                     /translation="MNKKENRMAIRQTVEKAVIRDEQNRRIQFAATNPIKEVLKKLHT
                     TLRGLEVDEVSVSRAKYGTNKVTHEKKKSLAKRLVGAFINPFTAILFCLALVSTITDM
                     IFPYFSLFGSVPEDFDSLTVVIILTMVFISGTLRFVQESRSGNAAEKLLAMITTTCTV
                     TRRNYEKKELPMDDLVVGDIVHLSAGDMIPADVRILDAKDLFVSQASLTGESEPIEKT
                     PNVSAQKESVTDYTNIAFMGSNVISGSATAVVVCVGDHTLFGSMASAVAGEAVETSFT
                     KGVNAVSWVLIRFMLIMVPLVFFVNGITKGDWLAAFLFGVSIAVGLTPEMLPMIVTTC
                     LAKGAVSMSKKQTIVKNLNSIQNFGAIDILCTDKTGTLTQDKVVLEYHLNVNGEDDTR
                     VLRHAYLNSYFQTGYKNLMDLAIIHKTEEEEAADSRLIDLSENYVKVDEIPFDFTRRR
                     LTTVVQDNKGKTQMVTKGAVEEMLSICAFAECNGGVQPLTDDVRYRILETVDNLNDKG
                     FRVLAIAQKSNPSPIGAFGVKDECDMVLIGYLAFLDPPKESTADAIKALKDHGVITKI
                     LTGDNDKVTRTICKQVGLEVRNMLLGSDLEQMGDAELARVAESTDVFAKLTPDQKARV
                     VSVLRENGHTVGFMGDGINDAAAMKAADIGISVDTAVDVAKESADIILLEKDLMVLEQ
                     GIIEGRKTYANMIKYIKMTASSNFGNMFSVLAASALLPFLPMMSVHLIFLNLIYDLSC
                     TAIPWDNVDEEFITKPRKWDASSVGSFMIWIGPTSSIFDFTTYIFMYFVFCPLFVSNG
                     VLFNDLPAHFSGAELAMLQAQYIGMFQAGWFVESMWSQTLVIHMIRTPKLPFVQSRAS
                     APVTLLTMTGIIVLTIIPFTVFGTMLGFVALPAAYFAYLIPCILLYMTLATSLKKAYV
                     RHFGELL"
     gene            complement(9165..9476)
                     /locus_tag="KR250_003091"
     CDS             complement(9165..9476)
                     /locus_tag="KR250_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3784 domain-containing protein"
                     /protein_id="HBH0068985.1"
                     /translation="MDFTCIFFGILFTLAGFWLAFGKGYRHLSLWKNMPQEEKDKINI
                     VPLCRNVGEMIALNGIIFLMKGFLPGFSNHWFVFAIIAWLIVAGFDVWYIEKSVRYRN
                     Q"
     gene            complement(9723..10400)
                     /locus_tag="KR250_003092"
     CDS             complement(9723..10400)
                     /locus_tag="KR250_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018597221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HBH0068986.1"
                     /translation="MFYTDFILRIALSLTLGFLIGLERQLTGHPAGIRINVLICMGTS
                     FFTLFPMLYGSDQVFRVGSSIISGVGFLCSGVIFKDRGTVRGMNTAATLWCTAAIGIL
                     ASTGMYAMAITAATILIGSNLILRPFARKFHPITSSDESEKQYRISVTCQEKAEQDIR
                     LLLINSNSCKTLFLNNLESGDVVGDKVEIVAEYCSVGKSKNNILEGIVGQVLVIPEVI
                     SAGWEVL"
     gene            complement(10538..11041)
                     /locus_tag="KR250_003093"
     CDS             complement(10538..11041)
                     /locus_tag="KR250_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006566797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBH0068987.1"
                     /translation="MKKNISYIPMDSALEIWLLEKLLRDPPDSDCAKDLLSGIQEMIR
                     GEVSSLLLGASEESDWPVGTLSDRILAGDLEIYPRGRKVLIKGSEVNLTPKEFDILYF
                     LVQNKGEVFTKEQIYRAVWEEDYILDNSNIMAFIRKLRKKIEPAPDCPTYILTIWGIG
                     YKFNDQI"
     gene            complement(11057..13720)
                     /gene="mgtA"
                     /locus_tag="KR250_003094"
     CDS             complement(11057..13720)
                     /gene="mgtA"
                     /locus_tag="KR250_003094"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016440611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HBH0068988.1"
                     /translation="MSKATLFDSRIKKYAYCRTSEIYRDIGSSPDGLSIEQIGSMREK
                     YGANSFNGRKNDTTMQRLRRAFINPFHVILFVLGIVSLVTDVFVASNFARNATTAIII
                     FSMIVISGVIRLIQELRAKSAAAQLDRLVHESVTVRRDGKLIEIPGEELVVGDIVLFS
                     AGDRVPADIRLTKITDLFISQAAITGESAIIEKSCRKLSYGEQDTLTQLENLAFMATT
                     VISGKGEGIVLAVGKDTLYGGFTKPDSDDKNSFKKGADSIAWVMIRFMAILVPIVFIL
                     LGITDGKWMESFAFALSVAVGLMPEMLPMVITACLAKGSLAMSKKQTIIKDINAMQSF
                     GSMDVLCMDKTGTLTNESILLEYYMDIFGNENAEVLDLAYLNSCYHSGVCNTIDNAIL
                     ACNTMPGREAHYANLLTKFQKADEIPFDYARKFVSTLVQDSNGNSQLIMKGDIEHIFS
                     RCNHVEYRGKLLPMGQDARQSVSSVVGEMLQDGMKVIAVARKNVGAQKKITPDDEWGM
                     TLVGYLSFFDAPKQTAKESVTALKRLKVIPKILTGDQAAIAVSVCRRVGIYTERILTG
                     TQLDEMTDCELKKAVEHTHVFAELTPGHKVRLVSAMQDNGHSVGFLGDGVNDIPALNE
                     ANVGISVDTAVDAAKDAADVVLLQKDLNVLEQGVMEGRKTFTNMLKYIKITASSNFGN
                     IFSIICASAFLPFLPMTSIQILLLNLLYDTLCIVLPWDNVDEEEMLSPRDWSGKTLRQ
                     FMLSFGPISSLFDIVTFLFLYYFLCPMLCGGATYLQLTDPSLQLQYVALFQTGWFLES
                     MWTQVLILHFLRTSKIPFFQSRASAPVISITLVGIIAFTSLTFVGGASLFGLTKLPLW
                     YFVFLLLVVSLYMLLNSVAKYFYKKKYQELI"
     gene            complement(13813..14022)
                     /locus_tag="KR250_003095"
     CDS             complement(13813..14022)
                     /locus_tag="KR250_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0068989.1"
                     /translation="MVKVKTKKKFVSTHMCQGEMPIYVRERYEKMLAVSIRAPNLEGM
                     VENFDKDLSRPNETDLIELKNAISK"
     gene            13972..14307
                     /locus_tag="KR250_003096"
     CDS             13972..14307
                     /locus_tag="KR250_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0068990.1"
                     /translation="MTHMSADKLLLGFHLDHVSNLNELLRKVAHNNICIMSDRMTKVA
                     VWKPSVKTIQLVCMEYLNYIIDHSVIYEKIQCANWNGTEETNSIFNNSPSPLITLIYS
                     CDLQQNKTT"
     gene            complement(14506..15408)
                     /locus_tag="KR250_003097"
     CDS             complement(14506..15408)
                     /locus_tag="KR250_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBH0068991.1"
                     /translation="MFKKNKKQTENVKEKKVRTVKVGTHKKTVIALWVVLIASVSFGV
                     YKNFTAIDQHTTHEKEVIELRLQDTNGIENFVKNFAKSYYTWSNSKEAIDARTQAISG
                     YLTKELQDLNMDTIRTDIPTSSAITDVIVWSIEQSGTDTFSATYEVDQQIKEGEQTTA
                     VKATYTVKVHVDAGGNMVIIQNPTLAPAIEKSDYEPKTPEADASVDADTINDATAFLE
                     TFFKLYPTATEKELAYYVSGNVLESIGRDYLYSELVNPIFTVDGDNVKVKVAVKFIDN
                     QTKATQVSQFELVLHKESNWKIIG"
BASE COUNT         4911 a         3230 c         2513 g         4847 t
ORIGIN      
        1 atattctttt tgtttttatg aaagtttttt agggggataa taatatatat gtttagagat
       61 aaaatggata gatgtacaca tatgttgact gcttatattg gtagttcata tgattattgt
      121 gattttatag atacacaact agatgatttt atactagagt acggagaaaa tgtggtagaa
      181 tcttgtttgc atcaagtgat ggtattagta agtaagtata attaaataga tttggttttc
      241 tagagaaccc tttttagttt tatctacttt aaatagcctt atgttagaaa gctaattaag
      301 atagatttct agtattcata attttatttt ttatttaaaa tacatataat aaagatgtaa
      361 atttgtatca aataataaat atgagaactt aaattctctt atctatgtga aaataaacat
      421 ctcttaaaat gaaattctgt aaatataaat gattttttag tttgaaggat tagattaaca
      481 aaattataaa tttgcaaata agctatatta aaaaatataa taatttttta tataacttat
      541 tcttttataa cttttaattt taaaagagga gaatgtatat aagagtattc aatgtactag
      601 aaaaaatcaa aagaaatatt agaagaaatg gatattgagt aatcaaagaa aagattttga
      661 tattacaaat gatatgtttc aagaaagcat tcctatcaca acaaaaatta ttcttgaaga
      721 catgccttca aatgatgaat taaatcatgt tttttcaaaa ggttgtgaac gaaaaatgaa
      781 aaagagaaaa attgtattaa tcacactact attaataggt gttttgttat taggaagtat
      841 cctatataat ctttttttag gaaaaacagc taatatttca atgctaaagg aatcttggaa
      901 ttttgatata ccaataccta acaaagaaat tgaagtattt gatactcaag atagtattaa
      961 tggagatgga caaagttatt ttattcaagg attttctgaa aaaaatttca aaaaagtttt
     1021 taatttaaaa ggtggtatag ttgtaagcaa agataatatt aatgaaatag aaaaatatat
     1081 tgataaattt aaaagagatt ctgttaacat taataaaagt aataaaaata aaattgaaga
     1141 agattttaaa aaatataagt tagaagttaa aaaagatgat aagtatatat ataagagaaa
     1201 ttatgagaac tacgtagttt taattataaa aaaagatgaa caaaaattat acagtttaat
     1261 atggaatcag taggtacata tgtattatta agaattataa ataataaaaa taaattttag
     1321 tcaatataca aaaacctttt aaattgtctg gaaatcacct cttcatttgt tataaaaaca
     1381 tacaacaaat gaagaggtga ttttatggtt ttaaaaaaaa taattataag tggatatgtg
     1441 ctttcacatg tttatcaaca tttgtaatgc ctatatttgc taatgaatta taaaaatatt
     1501 aaataatatt caactataga ttatgtaaat tactctatga aaaaaatgag ttgtataaaa
     1561 tctggctact gtagattgac tatttctttt aaaattacaa ttataataag ttttatagaa
     1621 ttaaaaataa aactttagag atttttgata acattgtaga ctaaatttgc caccgaaatc
     1681 gctttacagt ggcaaatcgg tggcaaatac aatatattat tagtatataa tctatataaa
     1741 cattactatt gtttgattat cttagctaca gtttccacat gtgtcgttac tactatcgga
     1801 acacatttta tcaacctcgt tgctgtcatg tgaacacaat tcattagtcc tatgccgtcc
     1861 tcttgcattt ttcctttgtg ttttaaattt agcaccatct acgctattct tcaagcctgt
     1921 tttataaaca aaggtcaact gtgcagggtc aacatttccg ttttcatcaa gtccacctac
     1981 aattactttt tcaacaatgc tttcaaacac atagcggtca aatttatcaa gaacctcatt
     2041 ctgttccaga gttttcttaa actctttcaa acgttttttt atgtctttct cattatcaga
     2101 agtttcttgt aacttttgtc tttcttcaac aagctgttct tgtttactta ctaaatcagc
     2161 atatttactt tcgtaggttt ccttatcaat aatttcttcc agacgcatat ccacaagttt
     2221 actttttttc ttctctaatg cgtctatctc cttttccgct tttgcaagtt gttttgacac
     2281 cacactacta ctaagagtat cgtccattct ctgcaaaaat tcttccagaa catctttatt
     2341 atcatcacat aataaacgat aactttctac aaatgcttct tcaatagctg tttctggaat
     2401 acctttgcta tgtttacaaa atttctttcc tttcttcgta gccgttacac attgccatat
     2461 aaccttgctg tattttgagc cactgtgcca attacgtctt gttaaagtac caccacaaaa
     2521 gccacattct agcatacagc taaatgcgta tttcctgcta tacttctcac gcttaccgct
     2581 gcctaccgtt gttcggttct tatttctccg attaagaatt tcctgtgctt tttcaaaaac
     2641 ttcttcacta ataatcggct catggtgatc tttcatataa aatttatctt cttcacccat
     2701 gttttcaagg cgacgtttgg aaatcggatc aacagtaaaa gttttgccta agagtatatc
     2761 acccttgtat ttctcattct taataattcc aataaccgta gatggtgccc atgtggaact
     2821 cccatacttc gttttataac ctagactttc aagctcctgt ccaataacag aaccaccagc
     2881 accatcaaca taacggttga aaatatatct gacaatctct gcttcttctt gatttacagt
     2941 tattgtctta tcctctgggt gataatcata tccaatacaa ccttgaaaac ctaccagctc
     3001 gccgcgcttc attttcattt tcaacccttt ttttacattg gctgaaatgt tctcgacttc
     3061 ctgctgtgca acagaactaa gaacaacaag caataactct ccatccattg taagtgtatt
     3121 gattttttca tcttcaaaat aaacggcgat atttctctct tttagcatac gaacatattt
     3181 caatgtgtct aaagtatttc tggcaaatct tgatatggat tttgtaaata ccatgtcaat
     3241 ttcaccattc atacagtcat ttatcatacg ctgaaaatct tctcgttttg ttacttgtgt
     3301 ccccgtaatt gcttcatcag cataaatgtc agctaacacc cattccttgt ttttcttaat
     3361 catgtcagta taatactgaa cttgtgattt ataactgttt aactgatctt cgctatctgt
     3421 acttacacgg caatatgcag ctacacgcaa acgctcaatg gcttttccac gggcttgtct
     3481 tgccaaaaca ttttttgctt ttattacttc aacttcctgc atagtacacc tccttatttt
     3541 gtgttccttt catattgtta ttgtactact ttaaaaaaca caattcaagc agttatgtca
     3601 gaaagcacct tatagtcttt catcagcctt tgttttacaa gagtgtattc tttttcagaa
     3661 atcagtttac gggcaagcag ttgttttaac atagcaattt gcatactata cctaattata
     3721 ttattcatta aaaccctcct tgctttttta tttatggaag taatcagacg gcttcggcag
     3781 ctccactgga ctttcaccat tcccattctg atgggtgaac cgtgtcatac gggtgtatca
     3841 ttattcagat atacgagtca tggcagcaac ctttccaaag atcgcttgcg gttcactggc
     3901 ataatcgctc gctgctttcc ccatttcagt ggtcgtggcg ttccagtccg ctggctcgtc
     3961 gtatatcaaa cgtatctgat tactgtattc acttgtcaaa gaacggaaaa gagttaaaat
     4021 gaggtggtag atactctctg ttatatttac ttcactttga cttcaaaccc taagattact
     4081 ttaatcaacg ctgcccgtat cctgcctttc agttccatat caagcacgat atacatattc
     4141 ccgtgttcgt catagaacgg gcgtaggctg gcttttgata tgtaagcgtc ataatggttc
     4201 aaaatctttt caattgccac ctcgttaccg tcagctgcag aactgatggt cgagaatgat
     4261 ggacactttt ttgtagactt catcatgttt ttattcctcc tcgtacatat ccttaataag
     4321 ctttaatgtg tgcagcctgt ttctacatac ggaaaatcgt tccatttcca taacctctgc
     4381 aatttcagcg tcggtcattt caagaaagta ggacataagc agaatgttac gtcggctttc
     4441 tgttaattct ttgatagctt cacataagcg ttcatcatag atatggactt ctgaaccaaa
     4501 tacatcaaat gacgtaaact caacgcaata ttcgtccatg gcaccgatct ggtttaactc
     4561 catttcagat aaatcacaga acggcgtttc acgtcttgca cgtctgccaa gctctcggtc
     4621 atagttcttt acagttcgtc ctacaacctt gcgagctaga caatcaaatt gaagtctgat
     4681 agcgccctcg aaagaagatg gtttcataat ctcaccccct ttccagcgaa agttgctaaa
     4741 gcgaaaagac ttctacctct tttcgcacta acactcacca tgaaaggtgg gatttgatac
     4801 ccaaccgcaa aaattttttg aaaaagtttt atgcagtaaa aaagcacaag ggcaatacag
     4861 acgtactgat cttgtgctaa acaaattatt cctgttatat gctgtaaaaa accacatata
     4921 ggagcattat gttccgtaga atttaaatgt ttttttaatg aactaccgat ggtcgaaagt
     4981 tgttgaatat gtaagtgttt catacggcta cccctagagc cgataaaaca agaccatcca
     5041 gtaatttgtc caacgttagg caaaaataaa acggcggcta tataaccgtc gtcttattca
     5101 aaagttcaaa aagaactaaa ctaaaccctc taaagcagta tgtatttata atcagtcttg
     5161 caaaataaca aagtagaatt gaaatataag gtagcaatcc ttgtgcatct aataaactta
     5221 taggggttac agcagaccac ggaataatat tttgtatact ttcagatcaa tatagtccaa
     5281 caaaaccaat tcccgatatt tagtaactaa aaagtaaact agagtagaaa acatttcttt
     5341 gcaaaacaca aataataagt aatggactca ttgacaatag attgagaaat ccagtttttt
     5401 agacttagat agattatctc acttgtaaat gatttaagtt ctttgacttc atatatagcg
     5461 gtataatctg tcaacgtgat cattacaaac cataatgcct atataacata ctttccaagc
     5521 aaaaacttta ttctattagt tggggaatac aaaatattta tcattgtgcg ttcaacatat
     5581 tctttttgta taaaaatgta attacaaaac accactcaat ataagataga taataatttg
     5641 aataggaatt atcaatctac gtaaccattc attacctgtc attgttgtta caatagattt
     5701 gggattctgt ttccattaca taattttttt atccttcttt cagatatctc ctcattttat
     5761 cgaagcttct tctacagaca tacattccat aataaaatct acctttgaat attatttcac
     5821 gatttcgtta tagacgcgta aacaaacaat aaaaaacgaa atttctttta agtaccataa
     5881 tttttatatc gaaattacaa ttatccactt atatacagca tttataggta attcaatgat
     5941 aaaaaaacac atactttact actaaattgc cacaattgaa tgcgccttga tatgataggg
     6001 agattccaca ataaaaaact gtgcatctcc cttgattcat tcatactgat atcttattta
     6061 taagactctt ttgtgtcttt ttttggtttc atggaccaaa atactacatt cattacaata
     6121 acaatcagca acacaactgc catagccacc caaaagccga tattaagacc aagccattcg
     6181 gtagttccaa aaagggtcat ccaaagttct ttccagttca tcattccacc tccttaaagc
     6241 agttctccga aatgacggac ataggctttt tttaaacttg ttgccaaagt catatacagc
     6301 agaatacatg gaatcagata agcaaagtat gctgcaggca atgcaacaaa tccaagcata
     6361 gttccaaaca cagtaaacgg aataatggtc agaactataa ttccagtcat tgttagcaaa
     6421 gtcaccggag ctgatgcacg actctgaaca aaaggtagct ttggagtacg aatcatgtga
     6481 atgactaatg tctggctcca catagattcc acgaaccacc ctgcttggaa catcccgata
     6541 tactgtgcct gaagcattgc cagttctgca ccgctgaaat gagcaggcaa atcattaaat
     6601 aatacaccat tcgatacgaa caatggacaa aatacaaagt acataaaaat ataggttgta
     6661 aaatcaaaga tagaactggt tggtccaatc cagatcataa aacttccaac actggaagcg
     6721 tcccatttac gaggtttggt aataaactcc tcatccacat tatcccaagg aattgcagta
     6781 caggataaat cataaattaa gtttagaaat ataagatgaa cactcatcat tggtaagaaa
     6841 ggtagcaaag cagatgcagc aagcactgag aacatattgc cgaagttgga tgaagcagtc
     6901 attttaatgt acttgatcat gttggcatat gtctttcgtc cctcaataat accctgttcc
     6961 aaaaccatca gatctttttc caatagaata atgtcagcag attcttttgc cacgtccacg
     7021 gcggtatcca ccgaaatacc aatatcggcg gctttcatag ctgcagcatc attaataccg
     7081 tcacccatga aaccgaccgt atgaccattt tcacggagaa cagaaaccac acgggctttc
     7141 tgatcagggg ttagctttgc aaatacatct gtagactctg ccactcttgc aagctctgca
     7201 tcgcccattt gttccaaatc agaaccgaga agcatattgc gaacctctaa tcccacctgc
     7261 ttgcagatag ttcgagtcac tttgtcgttg tcacctgtca aaattttggt tataacacca
     7321 tggtctttca gagctttaat agcatccgca gtagattctt ttggaggatc aaggaatgcc
     7381 agatatccaa tcagtaccat atcacactca tctttaacac caaatgcgcc gattggggaa
     7441 ggattactct tctgtgcaat agctagtacg cggaatccct tatcgttaag attgtccact
     7501 gtctccagaa ttctgtaacg aacgtcatca gtcagaggct gcactcctcc attacactct
     7561 gcaaacgcac aaatagagag catttcttcc acagcacctt ttgttaccat ctgggtctta
     7621 cccttattat cctgtaccac tgtagtcaga cgacgacgtg tgaagtcaaa aggaatctca
     7681 tctactttca catagttttc agacaggtca atgagacgcg aatctgcggc ttcctcttcc
     7741 tccgtcttat ggataattgc caaatccatc aggtttttat agccagtctg gaagtaacta
     7801 ttgaggtaag cgtgacgcag tactcgcgta tcgtcctcgc catttacatt cagatggtat
     7861 tccagcacaa ccttatcctg ggtcagcgtg ccagtcttgt cagtacagag aatatcaata
     7921 gcaccgaagt tctgaatgga attgaggttt ttaacgattg tctgcttctt gctcatggaa
     7981 acagcgcctt tcgcaagaca ggttgttaca atcatcggca acatttctgg tgtcaaacca
     8041 acagcaatag aaacgccgaa taagaaggcc gccaaccaat cgcccttggt gataccattg
     8101 acaaagaaca ccagcggaac cataatgagc ataaaacgaa tcaataccca agaaacggca
     8161 ttgacaccct tggtaaagct tgtttcaacc gcttctccag caactgcgga cgccatagag
     8221 ccaaacagag tatgatcgcc aacacaaaca acaacagctg ttgcgctacc agaaattaca
     8281 ttactcccca taaaagcaat gtttgtgtaa tcagtcacgc tttctttctg agcacttaca
     8341 ttgggtgttt tttcaatagg ttcactctca ccagtaagac ttgcctgact gacaaacaaa
     8401 tccttcgcat ccaaaatacg aacatccgcc ggaatcatat ctccagcaga aagatgcaca
     8461 atatctccca ccaccaaatc atccatagga agttcttttt tttcatagtt tctacgagta
     8521 actgtacaag tggtcgtaat catggctaac aatttctctg ctgcatttcc gcttctggat
     8581 tcctgtacaa aacgaagcgt accggaaatg aataccattg ttaaaatgat tacaacagtc
     8641 agggaatcaa agtcctctgg cacactgcca aacagagaaa aatacggaaa aatcatatct
     8701 gtaattgtgg atactaaagc cagacaaaat aaaatggcgg taaatggatt gataaatgcg
     8761 ccaaccagtc gttttgccag agatttcttt ttttcatgag tgactttatt ggtaccatat
     8821 ttagcacgac ttacggatac ttcatctact tctaagccac gaagtgtggt atgaagtttt
     8881 tttaaaactt ctttaatagg attggttgcc gcaaattgaa tgcggcggtt ctgttcgtca
     8941 cggatgactg ctttttctac agtctgacga atagccatac gattttcttt tttgttcatt
     9001 ggtcttacct ccttgaattt tcataggggc aaggaatatt atatgagcga cacggaatct
     9061 ccaaattctc atataatatt ctctgccttc gtggactgac atcagagtcc atgtctttca
     9121 cctcacaatc ttaaaaaaat atctatgtga aacctcaatg tggattattg gttgcgatag
     9181 cggacacttt tttcgatata ccagacatca aagccggcaa caatcagcca tgcgattatt
     9241 gcaaatacaa accagtggtt tgaaaatccc ggcaaaaatc ctttcattaa gaatataatc
     9301 ccatttaacg caatcatttc tccaacatta cgacaaagcg ggacaatatt aattttatct
     9361 ttctcctcct gcggcatatt tttccacagg gaaagatgac gatatccttt tccaaaggcc
     9421 aaccaaaagc ctgcaagagt aaataaaatt ccaaaaaaga tacatgtaaa atccatcgtt
     9481 tgttcctcct ttcaatgctt aaacctgttt cttagttttg tcagcttcag aagtttaatg
     9541 aagagcacaa taatagtaac agcaagaata aaccagatca caatactcac ctgataccat
     9601 atcttaaaga ttaccattct ttgttcaaag aaattccaac ccaaatagct acataagatt
     9661 gtaaatacag ggtagaaagc aaaaagaatt gctcgtttca aatcttcgat ttctctcctc
     9721 actcacaaca cctcccaacc agcactaatc acttccggaa ttaccaatac ctgcccaacg
     9781 attccttcta aaatattgtt tttggatttt ccgacagaac agtattctgc gacaatctca
     9841 accttgtcac caaccacatc accgctttcc agattgttca aaaagagcgt cttacaggaa
     9901 ttgctgttaa tcagcaataa gcgtatatcc tgctctgcct tttcctgaca tgtaaccgaa
     9961 atacgatact gcttttcaga ttcatcactg gaagtaattg ggtgaaactt tcttgcaaag
    10021 ggacgtagaa tcaggttgga accaattaaa atagtagcgg cggtgatcgc cattgcatac
    10081 attcccgtac ttgcaagaat tccaatcgct gcagtacacc ataaggtggc agcggtattc
    10141 attccacgta ctgtccctct gtccttgaaa ataacaccac tgcataaaaa accaacaccg
    10201 gatataatac tgcttccgac acggaatacc tgatccgaac cgtagagcat aggaaacaag
    10261 gtaaagaaac ttgtacccat gcagattaac acattgatac gaatacctgc aggatgacca
    10321 gtcaattgcc gttctaatcc aataagaaat ccaagtgtta aggagagggc aattctcaaa
    10381 ataaaatctg tgtaaaacat atgaaacacc tccctttaac tactaacagt atataaaaaa
    10441 tccaaataca atcctatggc taaagtcttg cgattttctt gcgatttggt aagaaaatag
    10501 acggactgaa accgaaaatg gtcttagtcc gtctttgtca tatctgatca ttaaatttgt
    10561 atccaatacc ccagatggtc aaaatatatg tgggacaatc cggagcaggt tcaatctttt
    10621 ttcgcaactt ccggataaaa gccataatgt tgctgttatc cagaatataa tcttcctccc
    10681 atacggcacg ataaatctgt tcctttgtaa aaacttcacc tttgttctgg acaagaaagt
    10741 acaaaatatc aaattccttt ggagtcaggt tgacttcaga accttttatc agtacttttc
    10801 tgcctcttgg ataaatttcc agatcccctg ccaggattct atctgacaga gtacccactg
    10861 gccaatcgct ttcttctgat gctcctaaca ataacgaaga tacctctcca cgaatcatct
    10921 cctgaatacc agaaagtaaa tccttcgcac aatcactgtc cggtggatct cttagtagtt
    10981 tttctaatag ccagatttca agagccgaat ccatcggaat ataactaata ttctttttca
    11041 tcatgaatgc ctcctcttaa atcaactctt gatatttttt cttataaaaa tatttggcta
    11101 ctgaattcag cagcatatag agagatacca ccagcagtaa aaatacgaaa taccaaagag
    11161 gtaatttcgt cagtccaaac aacgacgcac cgccgacaaa tgtaagggaa gtaaaagcaa
    11221 taatccccac tagagttata gaaatgaccg gtgcagatgc cctgctctgg aaaaaaggaa
    11281 tcttggaagt tcgcagaaaa tgtaaaatca aaacctgtgt ccacatagat tccaaaaacc
    11341 atccagtctg aaacagtgct acatactgaa gttgcaggga aggatctgta agctgcagat
    11401 aggttgcccc tccacatagc atcggacata ggaaataata caaaaatagg aaggtgacaa
    11461 tatcaaacag agaactgatc ggtccgaaag acagcataaa ctgtctcagt gttttgccgg
    11521 accagtctct tggggacagc atctcttcct catctacatt gtcccaagga agcacaatac
    11581 acaacgtatc atacagcaga ttcaaaagca gaatctgaat tgaagtcata ggcaggaacg
    11641 gtaaaaaggc actggcacaa atgatggaaa aaatatttcc aaagttagag ctagcggtga
    11701 ttttaatata tttcagcata ttggtaaaag tttttcgccc ctccattaca ccttgctcca
    11761 aaacattcaa gtccttttgc agcaaaacca catcggcagc atcttttgct gcgtccactg
    11821 ccgtatctac agagattccc acatttgctt cattcagtgc cggaatgtcg ttgactccat
    11881 caccaagaaa tcccacggaa tgaccattat cctgcatagc agagacaaga cgcaccttat
    11941 gtcccggagt aagctctgca aaaacatgag tatgttccac agccttttta agttcacagt
    12001 ctgtcatttc atccagttga gtaccagtta agatacgctc tgtataaata cccacccggc
    12061 ggcaaacaga cacagcaatc gctgcctggt cccctgtcag aatcttaggg ataactttta
    12121 atcttttgag tgcagttaca gactcctttg ctgtctgctt tggtgcatca aagaaggata
    12181 gatagccaac tagagtcatg ccccattcgt catcaggagt tattttcttt tgcgctccca
    12241 cattttttcg tgccaccgca atgaccttca ttccgtcctg taacatttca cctactacag
    12301 aagatacact ctgtctcgca tcctgcccca tggggagaag ttttccacga tattctacat
    12361 ggttgcagcg tgagaatata tgttcaatat cacctttcat aataagctga ctattcccat
    12421 tgctatcctg tacaagtgta ctgacaaact tacgagcata gtcaaaggga atttcatcag
    12481 ctttttggaa ttttgtcaga agatttgcgt aatgggcttc ccgccctggc atggtattgc
    12541 aggcaagaat ggcattatca atggtattgc agacaccaga atgatagcag ctattcagat
    12601 aagcaagatc aagaacctct gcattttcat tcccaaaaat atccatataa tattccagca
    12661 ggatgctttc attggtaagt gttcctgttt tatccataca aagcacatcc atgctaccaa
    12721 agctctgcat ggcattaata tccttgataa tggtctgctt tttgctcata gcaagacttc
    12781 ctttggcaag gcaggctgta ataaccatag gaagcatttc cggcatcaga cctacagcaa
    12841 ctgacagggc gaaagcaaaa gattccatcc attttccatc agtaatcccc agtaatataa
    12901 atacaatggg aactaatatc gccataaaac gtatcatcac ccaggcgatg gaatcggctc
    12961 cttttttaaa ggaattttta tcatcagaat ccggtttggt aaatcctcca tacagggtat
    13021 ctttgcctac tgccagtaca atgccttccc cttttccact gatgactgtg gttgccataa
    13081 aagcgaggtt ttcaagctga gtcagtgtat cctgctctcc gtaactgagt tttcggcagc
    13141 tcttttcaat gatggcgctc tcgccagtaa tcgcagcctg agagataaag agatccgtaa
    13201 tcttcgtcaa tctgatatcc gccggaacac gatcacctgc agagaatagt acaatatccc
    13261 ctaccactaa ttcttcgcca ggaatctcta ttaactttcc atcacgcctc actgtgacgc
    13321 tttcatgaac taggcgatca agctgagcag cagcgctctt tgctcgcagc tcctgaatca
    13381 gacgaattac gccactaatt acgatcatgg aaaagatgat aatggcggta gtagcatttc
    13441 tcgcaaaatt ggatgctacg aaaacatccg taacaagaga aacaatgccc agaacaaaaa
    13501 gaatcacatg aaacgggtta ataaatgccc gtctcaaccg ttgcatcgtc gtatcatttt
    13561 tccgcccatt gaagctgttc gctccgtatt tctcccgcat tgaaccgatt tgctcaatgg
    13621 ataaaccatc cggagatgag ccaatgtctc gataaatttc tgatgttcta cagtatgcgt
    13681 attttttgat acgactatca aatagtgttg cttttgacac gaaacatccc ctcctttata
    13741 agtataagta ataacatttt aacacagtgt tctgaaaatg ctatatttat attcttgctt
    13801 ttttcttgca atttactttg atatggcatt tttcagctcg atcaaatccg tctcgttagg
    13861 gcgagataaa tccttatcaa agttttcaac cattccttct aaatttggag cacggatgga
    13921 tacggcaagc attttctcat agcgctctcg cacataaata ggcatttccc cttgacacat
    13981 atgagtgctg acaaacttct tcttggtttt caccttgacc atgtatcaaa tttaaatgaa
    14041 ctacttagaa aagttgctca taataatatt tgtattatga gtgatagaat gacaaaagtg
    14101 gcagtatgga aaccgagtgt aaaaacaata caattagttt gtatggaata tctgaactac
    14161 ataattgacc actctgtaat ttatgaaaaa atacaatgcg caaattggaa cgggacagaa
    14221 gaaaccaata gtatttttaa taattcccct tcaccattga ttactcttat ctattcttgc
    14281 gatttacaac aaaataaaac aacataaatt ttaaatacgt tgactggcat aattcatatg
    14341 tatagtaagt ctacaattaa gatgcgcttt ttctaatcca aatatattaa gaaaaaagtt
    14401 ttgcgaagcc attaatactt tgtttaagat gtctttcata aatataaaga ataatgctct
    14461 taacaaaagc gtgcaaattc ctaaagaaat gcacgctttc aataattatc ctataatctt
    14521 ccaattgctc tccttatgta gcactaattc aaactgcgat acctgtgttg cctttgtctg
    14581 attatcaatg aatttcacgg caaccttgac tttcacattg tccccatcaa ccgtaaagat
    14641 agggtttacc aattcagaat agaggtagtc cctgccgata gactcaagca catttccaga
    14701 cacatagtaa gcaagctcct tttctgtggc tgttgggtat agcttaaaga aagtttccag
    14761 aaatgcagta gcgtcattta tggtatccgc gtctacactt gcgtctgctt ctggtgtctt
    14821 tggttcatag tctgatttct cgattgctgg tgcaagggta gggttctgaa taattaccat
    14881 attcccgcca gcgtccacat gaacttttac ggtataggtt gccttaaccg ctgttgtctg
    14941 ttccccctct tttatctgct gatctacttc gtaggtagcg gaaaaagtgt ccgttccaga
    15001 ctgttcaatg ctccatacaa tcacatctgt aatggcagag ctggtcggta tatccgttct
    15061 aatggtatcc atattcaagt cctgcaattc ctttgtcaga taaccgctga ttgcctgcgt
    15121 ccttgcgtca atcgcttctt tgctgttact ccatgtgtaa taggactttg caaaattctt
    15181 cacgaaattt tctatcccgt tggtgtcctg caagcgaagc tcaatcactt ctttttcatg
    15241 ggtcgtgtgc tggtcaatag cggtaaaatt cttatatact ccaaagctca cgcttgcaat
    15301 aagcaccacc cacaatgcaa tcacggtttt cttatgtgtg cctaccttga cagtacgtac
    15361 ctttttttct ttgacatttt ctgtctgttt cttattcttc ttaaacatat ttttcaagtc
    15421 ctttctattg ttttactcgt cctgcaccga ttaagtgctg ttgccagtaa ctactactta
    15481 ggtctgtata tcctatcggg t
//
LOCUS       DAEPIN010000080        15324 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.080, whole genome shotgun sequence.
ACCESSION   DAEPIN010000080 DAEPIN010000000
VERSION     DAEPIN010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15324
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.080"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            complement(228..608)
                     /locus_tag="KR250_003098"
     CDS             complement(228..608)
                     /locus_tag="KR250_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBH0068992.1"
                     /translation="MCLKKLSKLELVIMKFIWNLDIKTNSYEIIDYMKEEHNLPEKVA
                     LKTLSKLSKKRFLYVQETGKCMYYTVAIKEKAYLEFISRNVQNLLKNNFIRNLLVSFH
                     EEELTEEKIKSLENWVVNWEEAYV"
     gene            complement(809..2002)
                     /locus_tag="KR250_003099"
     CDS             complement(809..2002)
                     /locus_tag="KR250_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HBH0068993.1"
                     /translation="MLTSLALIFLLGMASGGIFKRIKLPNLLGMLLTGIILGPYVLNL
                     IDNSILDISSDLRKIALIIILTRAGLSLDINDLKKVGRPAVLMCFIPATFEIIGMIVL
                     APKLLGVSILEAAVMGAVVGAVSPAIIVPKMLKLMEEGYGTEKSIPQMLLAGTSIDDI
                     FVIVMFTVFTGLAQGNSISAISFLQIPVSIILGVIVGAVIGIYLAVFFKKVHMRDSAK
                     GVLLLSISFLMISLETALEGIVPFSGLLAVMSIGIFLQIKYRVVARRLSIKYSKLWVG
                     AEILLFVLVGATVDISYAFKAGIGAVILIFGVLLFRMVGVFFCLIKTNLTIKERLFCM
                     IGYIPKATVQAAIGGVPLAMGMASGQLILTLAVLAILITAPLGAFGIDVTYKKLLTSV
                     KGENK"
     gene            complement(2065..3321)
                     /locus_tag="KR250_003100"
     CDS             complement(2065..3321)
                     /locus_tag="KR250_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HBH0068994.1"
                     /translation="MIEKFKTLIWIKMIIRIKSVYKTYGGLFFLGLIGIPVGAIIGLI
                     DTIFGTVLLKVTDIRETYPMYLIPFLAVVGVVIAYCYFKFGGKSSKGMNLIFEVGHGE
                     EEIIPLRLVPFIISGTWLTHLFGGSAGREGVAVQIGATFSHWVGKRLPIKNASSIFLV
                     TGMAAGFAGLFETPIAAILFAMEVLVAGSLEYQSLFPAFTASFTASAVSKALGLEKFS
                     FALSSKVVFDLSIFWKLIVLGIIFGMVGGAFAWCLKLSKRKIGNRLKNPMIRIAIIGV
                     CLSVLFLLFYKGRYSGLGTNLIQNSFYGGEIYSFDWLLKFILTILTLSAGFQGGEVTP
                     LFSIGASLGVLLAGFFNLPIELVAALGYASVFGSATNTFFAPVFIGAEVFGYSYLPYF
                     FVVCAISYIFNMDKSIYSLQKISTKQ"
     gene            complement(4117..4851)
                     /locus_tag="KR250_003101"
     CDS             complement(4117..4851)
                     /locus_tag="KR250_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBH0068995.1"
                     /translation="MNLAKGLDVLKISSNVFGEDKVMYIPVIYTEDDATLIDTGLPGQ
                     GDLIIDALNKSNTSFDKLKNIIITHHDIDHIGNINYLREKSKNNIKVYAYKSEISYIT
                     GEETPFKLYMLEQMVDKIDDKMLSMLNVMGLGFKSSYTKVDVSLDNHEKLNLGEEIEV
                     IHTGGHTRGHICLYLKESKVLIAGDLLQVENGELKPVDVMYSNKQELKDAIKNISNYD
                     IETIVFSHGGLYQRNIIGTLKNLIIE"
     gene            complement(5857..6495)
                     /locus_tag="KR250_003102"
     CDS             complement(5857..6495)
                     /locus_tag="KR250_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HBH0068996.1"
                     /translation="MLIHLVKKDILIVKKYVLLILFIAIGIPLFILWRLPPFAQILGF
                     VLSIIFSEFMLCQYLLLKESQYSKASVLLCSTPYPRRGLVQSKYVLFAMVFIYCTLVY
                     WIENLLIPQIGTFDLTYILIVLLISSIIYGVYMPIQYKLGYETTKFFFVMIIMASSFV
                     LPLTIAHSNFEIPFIYQYPSMIVNIVLFLLSLIILLASMFISIKIFSNKELV"
     gene            complement(6498..7355)
                     /locus_tag="KR250_003103"
     CDS             complement(6498..7355)
                     /locus_tag="KR250_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0068997.1"
                     /translation="MENILEVYNLNKTYTDFSLSNISFALPEDCITGFIGVNGAGKTT
                     TIRSILDVVKRDSGKIKIFGKDFDGNEKELKNRIGVVFDDSYFYEELSMAEMKSIIAP
                     SYDNWCEEDYQQNMKRFNLNPKQKIDTLSKGMKMKFALALALSHKAEFLIMDEPTSGL
                     DPLIRSQILDILTGYMKKCGRGVFFSTHITSDLDKIADMIVMIDNGRIILEREKDTLL
                     DEHRKVKGDIQFLNDETKKLFLNLEITGYGFIGITSKINEVQKNMPDILIERPTIEDI
                     MLAYIERRE"
     gene            complement(7357..7731)
                     /locus_tag="KR250_003104"
     CDS             complement(7357..7731)
                     /locus_tag="KR250_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBH0068998.1"
                     /translation="MKVLISNTSDKPLYQQIKEQIKEAIFTEELKEGDAMPSIRNFAN
                     DLKVSILTIRRVYEELENEGFLTRQVGIGTFISTGNLEILRDSKRRIVEQKMADMIKD
                     AKALGITQIELQEMMDILYEED"
     gene            complement(8880..10070)
                     /locus_tag="KR250_003105"
     CDS             complement(8880..10070)
                     /locus_tag="KR250_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HBH0068999.1"
                     /translation="MSDMLLWFSVVLVLFAIGDLIASKTKAKVSAVFVTLLLFLILFV
                     TKAIPADIIEKAGMTAAASWSVPMIMFSMGTMLNVKQFIDEWRTVLTAWLGIVAVIVC
                     VSLCIPLFGKSTVLTSIPVINGALPATTIMTQAALEKGLTLAAATATVVFAIQKFIGT
                     PIASRAALQEANRLLVEYHEAKSKGIDLANVDTDKKEAENTGTKVKQAFCEKYDKYYS
                     TNVCIFFIALFSYLGYELSEIIHVNYSIVCLVVGVIVTRIGIVPKDILEKGKIKGFIN
                     MVVFAAVIPSLAKVSLTDLISLFVPIVGMFAASIIGIFLMMKVLPGWKIIGSKPLAFG
                     VGFCQMLGFPTTYLISNEVCNAVGETEEERAYLMSKIMPKLVVGGMACMISIVVAGLM
                     VPML"
     gene            complement(10086..12041)
                     /locus_tag="KR250_003106"
     CDS             complement(10086..12041)
                     /locus_tag="KR250_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HBH0069000.1"
                     /translation="MEKIKIENSDIFEYIFPHDISCSPDGKHIAYIISNINEEKDCYE
                     HDLYVMDIKTEKQIHMTQTKDVTSFSWISNTELLFTSKRNKPKSGTTDFYTISIEGGE
                     AKKAFSIPKACSVPVSLGNKLWLLTTKNPTDSKKSEPDRAVEGVDYWTFTDKPFIRDG
                     ENFSQRRRVTLELYQEGENITKAITPKFCEVAGVDVSSDKNRILYTGQIYEDCATPFS
                     GLWEYHIDSGETKELVPQGKYQISLAKYIGKDKVMLQASTLDRSITQNHDIFILDLST
                     NEINMIASPDGMYATLLDVDAVYGGGRSNKVIGDKFIGARICRTMTEFNEFDTKTGNI
                     RIITKVDAFTSFDIYDNTMYTVMLKDYELAEIYSIDMTTGTMKKMTAFSKPYLDTHKV
                     SLPEKLTFVAKNKEEVDGFVIPPIDAKEGEKYPAVLFIHGGPKWAYGYMFTHLKQCVT
                     SKGMYVIYCNPHGGDGYGEKFLEMVERWGYVDYEHLMEFVDTCIEKYPGIDADRLGVA
                     GGSYGGYMTNWIIGHTDRFKAAVSQRGISNLITASLIIDFGDRIMKQTCGDKTPWNHE
                     EVLWNHSPIKYVKNVKTPTLFLHSDRDYRCFMGDTFQMFTALKQLGVDTEMYLFHGDT
                     HGLSRNGRPSNRIARANAIVDWFERYL"
     gene            complement(12058..14124)
                     /locus_tag="KR250_003107"
     CDS             complement(12058..14124)
                     /locus_tag="KR250_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HBH0069001.1"
                     /translation="MKEVVESLKKITKERIENTMKKLFTSETFLEYQFISDCQISPDG
                     GYTAFIVKKADIKENGYTSQVYILNNKTGELKQITSINSVGAYAWEDENTILFPALRN
                     EKVKEAVKNGKQCMSYYALSLNGGEAEELFRLPIKGGKLNPIGKGLYAVIDSYDNDRP
                     IVEGLPEEEQQKKINAYNKRHYEHFKEIPYAVNGEGYISRKRKRLYVYDSNINELKAI
                     TAPMFNVVGMKISDGKILYIGQEFKDVKGLKNGVYVFDTNTNTNVCILEKDKYIIKGF
                     ELYQNQVILNLTDALSYGNGENGDFYTIDIDTKEMKLLSGHQHHCIGNTVTSDVKMGA
                     GQTTKVDGEYIYYTSTVDMDCIIERIHIPTGKQEKVTQTGSVDFIDVKDGNIVCVGCL
                     GNGLPEVYTVENGTLCKKTHLNDHILEEYKISVPEYIESKGSSKWEIQGYVYKPVDYE
                     VGKKYPAVLAIHGGPRLTYGPYFMHEIQVFTSAGYFVFFCNPRGSEGRGNAFADIRKQ
                     FGDIDYIDFMEFTDTVLEKYTDIDKTKLAVEGGSYGGFMTNWIIGHTNRFAVACAQRS
                     IANWSGMEGTTDIGYYFCKGQTGASHMENHELQWKQSPLAYADKCVTPTLFLHGEKDY
                     RCYMQEAFQMFSALKIHGCPTKLCLFEGENHELSRSGRPKQKLQRLVEMLDWFSVYIK
                     KDNIGQ"
     gene            complement(14451..15101)
                     /locus_tag="KR250_003108"
     CDS             complement(14451..15101)
                     /locus_tag="KR250_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBH0069002.1"
                     /translation="MKVKSNLDEILYHKIIESLIRGEYSVGQKILLNDLCEKFEVSRT
                     PVVQAVKMLNKDGVLTIMTNGKVYVPEYEYDMVKQVCETRTLIETYALEKMMQEEEEV
                     FQQKLDVIKRYSDKCEEYYRQEKSVELALADLELHKAIVEGANNEILNDVYVGIQGRF
                     IVVNYLIRPLKNRNYEGTVQDHFEILKFIEDRDTKKAVDKLRNHIQGTIKRFCEDE"
BASE COUNT         5271 a         2717 c         1718 g         5618 t
ORIGIN      
        1 ataaagaata gaaacaagtt ctagattact atagagatga catatcaaaa tatttttaaa
       61 aagcaattta agtattagaa gtaattgaag ttagtaaata taaatgcgtt tacttttaag
      121 tattgcttaa gggaataaaa aataaaaata aactctgcaa ccatctaaaa atagttgcag
      181 agcttacaaa accaaaatta tctaaattgt aataaaaata ataacattta tacataagct
      241 tcttcccaat ttactaccca gttttctaac gactttatct tttcctctgt tagctcctct
      301 tcgtgaaatg atactaataa attccttata aaattattct tcaaaagatt ttgcacattt
      361 cttgatataa actctaaata tgccttttct ttgattgcaa ctgtataata catacacttg
      421 cctgtttctt gaacatataa gaatcttttc tttgatagtt ttgataaagt ttttaaagct
      481 actttttctg gtaaattatg ttcttctttc atataatcaa taatctcata tgaattggtt
      541 tttatatcta aattccaaat aaatttcata ataactaact ctaattttga taattttttt
      601 aaacacatag ttatcactcc gcttctttat ctatataatt aaatattaca ataataattt
      661 cacaaaatca attctaataa tcataacata ccttaaaatc tgttcttaac ttctaccttc
      721 ttaatctccc aaatgatttt tttatattgt attcaatagt ataatccttt tccaaaatat
      781 aacttaccag ttaaaataat ctttattttt acttattctc gccttttaca gaagttaata
      841 attttttata agttacatca attccaaaag ctccaagagg tgcagttata agtatagcta
      901 gaactgctaa cgtcaaaata agttgtccag atgccatccc cattgcaagt ggtacacctc
      961 ctattgccgc ctgtactgta gcctttggaa tatagccaat catacagaac aatctttcct
     1021 ttattgtcaa atttgtctta ataaggcaga agaatactcc taccatacgg aataaaagaa
     1081 ctccaaatat taaaattaca gctccaattc ctgctttaaa tgcataactt atatctactg
     1141 ttgcccctac caaaacaaac aagagaattt cagcaccaac ccatagtttc gaatacttta
     1201 tagacaatct tcttgcaact acacgatatt ttatctgaag gaaaatccca atactcataa
     1261 ctgctaataa tccagaaaat ggcacaatac cctctaaagc agtctctaaa cttatcatta
     1321 aaaatgagat acttaaaagc aacacccctt tagcagaatc tctcatatga accttcttaa
     1381 aaaatactgc taaatagatt ccaatcactg ctccaactat tacacctaat ataattgata
     1441 ctggaatttg taaaaagcta atagctgata tactatttcc ttgtgccaat cctgtgaata
     1501 ctgtaaacat cacaattacg aaaatatcgt ctatagaagt tcctgctaaa agcatctgtg
     1561 gaatactttt ttctgtacca taaccttctt ccattaattt aagcatttta ggtacaataa
     1621 ttgcgggaga aactgctcct actactgcac ccatgactgc tgcttccaat atagaaacgc
     1681 ctagtaactt tggtgctaaa actatcattc cgataatttc aaaagtagct ggaataaagc
     1741 acataagtac agcgggtctt cccactttct tcaaatcgtt tatatctaaa gataatccag
     1801 cccttgttaa tataataatc aatgcaatct ttctaaggtc agaagaaata tctagtattg
     1861 aattatcaat caaatttaaa acatatggac ctaatataat tcccgtaagt aacattccca
     1921 ataagttagg taattttatc ctcttaaaaa tacctcccga ggccattcct aataaaaaaa
     1981 tcaaagctaa acttgtcaac atattcctac cctatccttt ctctaatatt ctttctcttc
     2041 taatcataca ttatgttgtt ttatttattg ttttgttgaa attttttgta gtgaataaat
     2101 agatttatcc atattaaata tatatgaaat agcgcatact acaaagaaat aaggtaaata
     2161 gctgtaccca aaaacttctg cccctataaa tacaggtgca aaaaaagtat ttgtagcact
     2221 accaaagaca ctagcatagc caagcgctgc aactaattca attggcaaat taaaaaatcc
     2281 tgctaacaat acaccaagac tagctccaat ggaaaacaaa ggtgtaacct ctcctccttg
     2341 aaaacctgct gacaaagtca gtattgtaag tataaacttt aacagccagt caaatgaata
     2401 aatttctcca ccatagaaac tattttgaat taaatttgtc cctaaacctg aatatcttcc
     2461 tttataaaat aaaaggaata atacactaag acatactcca ataatagcaa tccttatcat
     2521 aggatttttt aatctattcc ctatttttct ttttgacaat ttcagacacc atgcaaatgc
     2581 tccaccaacc attccaaaaa taattcctaa aacaataagc ttccaaaata ttgacaagtc
     2641 aaatacaact ttagaagaaa gtgcaaaaga aaatttctct aaccctaaag cttttgatac
     2701 tgcacttgct gtaaaagaag cagtaaacgc aggaaaaagc gattgatact ccagtgaacc
     2761 agcaaccaaa acctccatag caaatagtat tgctgctatt ggtgtctcaa ataatcctgc
     2821 aaaaccagct gccataccag ttactagaaa aatactagaa gcatttttta taggcaacct
     2881 tttacctacc caatgtgaaa aagttgcacc aatctgaaca gcaactcctt ctcgtcctgc
     2941 actaccacca aataaatggg ttaaccatgt acctgatatt ataaatggaa ccaatcttaa
     3001 aggaataatt tcttcttctc catgccctac ctcaaatatt agattcattc ctttactact
     3061 ttttcctcca aatttaaaat agcaataagc aataacaact cctactactg ctaaaaatgg
     3121 tatcagatac attggatatg tttctcgaat atctgtaact tttagaagaa ccgttccaaa
     3181 tatggtatct ataagaccta taattgcacc aactggtata cctatgagtc ctaaaaaaaa
     3241 tagtccacca tatgttttat aaacgctttt aattcttatt atcattttaa tccatattaa
     3301 agttttaaat ttttctatca ttttaattcc tttctatttg catcataaga ttttttgaaa
     3361 gttttattac atcaactgta gtcaatgtaa tgactatcat aattgttttt taaattgata
     3421 ggatataggt acactcttaa cccattctta ataaaataat taacatataa tctactattt
     3481 tttctattaa ttaaatattt cttcaaattt aaaaaataaa aatttagtaa gtacttcacc
     3541 tccattttat cgatttaaac aacaaaaaaa gctggtggca tctatgatat catagaagtc
     3601 atcagctcat tggcagttta ggatattatc atccaaggga gaacctcatt cccataatta
     3661 ttatataatt ttttattttt catatgtcta ttttatgtaa attgtatttt taatgttata
     3721 aataatataa cctatcaatt aaaatattaa agtctctaat attatgagtg ttcaatactt
     3781 catattattt ttatgatata taatttaaat gattttcttt actttgcatt atgatacatg
     3841 atttaaatat ttttcttcac tttgtatact aaagtttatt taaaatattt tctttcctat
     3901 tatactatgc tgttaaatat ttttcaactg aagtattgta gaactttaaa attactataa
     3961 aaaattatat tctaaaatac ttgtaatact acatatcacc cttaacactt aagtataatt
     4021 ttaaagcaaa attcttatta cttatattta attttgattc tacatcttta gatataattt
     4081 taagtaattt aagaaattat tgtaaatcat atagtactac tcaataatta ggtttttcaa
     4141 tgttccaatt atgtttcttt gatataatcc accatgacta aaaactatcg tttctatatc
     4201 ataattactt atatttttaa tagcatcttt taactcttgt ttgttgctgt acatcacatc
     4261 aacaggctta agttctccat tttctacctg taacaaatct ccagcaataa gtactttaga
     4321 ctcttttaaa taaagacata tgtgacctct agtgtgacct cctgtatgta taacttctat
     4381 ttcctcacct agattcaatt tttcatggtt atctaatgac acatctactt tagtatatga
     4441 tgatttaaat ccaagtccca taacatttag catactaagc atcttatcat caattttatc
     4501 taccatttgt tccaacatat aaagtttaaa aggagtttct tctcctgtta tatagctgat
     4561 ttcacttttg tatgcatata ctttgatatt attcttagat ttttctctta gataatttat
     4621 gtttcctata tggtctatat catgatgagt gattattata ttttttaact tatcaaagct
     4681 cgtgttactt ttattaagag catcaattat taaatcacct tgacccggaa gacctgtgtc
     4741 aataagtgtt gcatcatctt ctgtgtagat tacaggaata tacataactt tatcttcacc
     4801 gaatacattt gatgaaattt ttaaaacatc taatcccttt gctaaattca taatttttct
     4861 ccttataatt acttaattag aggtcaattt aattaaagaa attgagaaaa tgtagatttt
     4921 cctctagtta tattttatta attaaaaatt attaatacct agtatatcat ttaagttgaa
     4981 ggattactcg acttgatttt tttgttattt tatggtatag taattaatat agaagtaagt
     5041 aattgcaagc tttgcaatta cttttatttt tttctaataa ctattttatt tttgataatt
     5101 cgtataaaat taaaagtaaa gtaatataga aaatatgaat atagaaaaac tttaataaga
     5161 aaataaatta tgaataaata aaagaaacgc aatactgtat atagatgatt atttagaaaa
     5221 ataaaatctc ttttaataaa aataagcttc aaaataaact tttagttcat aaagcttagg
     5281 agtaactaaa ttgagtgcaa gtgtatctac tgttcaaaat aaatagttca taaagcactc
     5341 ttttttatat taaatcaatt atattttcgt catttcagca atgaaaaaga agcttatctc
     5401 tattttgaga cagcttcttt ttattaacgt cctacttttt attagttttc taaaattaaa
     5461 aagctttatt tataggattc cttagattta tattttgacg atataatttt ttaaaaacac
     5521 cttaaaagat ggtatacaat acacacaatc atactcataa ttgaaaaata atcaatggaa
     5581 aacaaaatta ctggatattc cctattaaaa aatacaaatt gattcacaag aaacatataa
     5641 tctataatat tatgatgtta ctctcccaca ccaagtaact ttattttcaa gtaacctttt
     5701 cataccatct aacttcactc aataaaaatg gcaatataaa tatatactct attttttact
     5761 tttaaatatc tgacaagctt tatatactat tggaagtaag atacaaagta aaacagccca
     5821 atctggtaaa tgtggcatat aaaagtcccc cttaccttat acaagctcct tattactaaa
     5881 aattttaata gaaataaaca ttgaagctaa cagtataatt aaacttaaaa ggaacaatac
     5941 aatattcact atcatggatg gatactgata tataaatgga atttcaaaat tgctgtgagc
     6001 tatagttaat ggtaatacaa atgatgaagc catgattatc atcacgaaaa agaattttgt
     6061 tgtttcatat cctaatttgt attgaatagg catataaact ccataaataa tagaacttat
     6121 taataaaaca atcaaaatat atgtcaagtc aaaagttcct atttgtggaa taagtaaatt
     6181 ttcaatccag taaactaatg tacaatagat aaataccatt gcaaataaaa catacttaga
     6241 ttgaactaag ccacgtctag gataaggagt tgaacaaagt aatacagatg cttttgaata
     6301 ttgactctcc tttaataata gatactgaca cagcataaat tctgaaaata taattgatag
     6361 tacaaatccc aatatttgtg caaatggagg taaacgccat aatataaata atgggattcc
     6421 tattgcaata aataatatca acaatacata tttttttaca atcagaatat cttttttaac
     6481 taaatgaatc aacataatta ctctctcctt tcaatatatg caagcataat atcttcaatt
     6541 gtaggacgct ctattaaaat atctggcata tttttttgca cttcattaat ttttgaggta
     6601 atccctataa aaccataacc tgtaatttct aaatttaaaa ataatttttt ggtttcatca
     6661 tttagaaatt gaatatcccc ttttactttt ctatgttcgt ccaatagagt atctttttca
     6721 cgctctaata taatacgtcc attgtcaatc ataacaatca tatctgctat tttgtctaaa
     6781 tcagatgtta tatgagtgga aaagaaaaca ccacgaccac atttcttcat atatcctgtt
     6841 agtatgtcaa gtatctgact acggattagt gggtctaaac cacttgtagg ctcatccata
     6901 attaaaaact cagctttatg cgataatgct aatgctaaag caaatttcat tttcattcct
     6961 tttgaaagtg tatcaatctt ttgttttggg ttaagattaa aacgcttcat attttgttga
     7021 taatcttctt cacaccaatt atcatatgaa ggtgcaataa tacttttcat ttctgccatt
     7081 gacaactcct catagaaata tgaatcatca aagacaactc caattcgatt ttttaattcc
     7141 ttctcatttc catcgaaatc tttaccaaag atttttattt ttccactatc acgctttacg
     7201 acatccaata tcgaacggat tgttgttgtt tttcctgctc cattaacacc tataaatccg
     7261 gttatacaat cttctggtaa tgcaaagctt atattagata atgaaaaatc ggtataagtc
     7321 ttatttaagt tataaacttc taaaatattt tccatattaa tcctcctcat atagaatatc
     7381 catcatttct tgtaactcta tctgtgtaat tcctagtgct tttgcatctt taatcatatc
     7441 tgccattttt tgttcaacta tacgtctctt tgagtctctt agtatttcta aatttccagt
     7501 tgaaataaat gttcctattc caacttggcg tgtaagaaag ccttcatttt caagttcttc
     7561 atacactcgt ctaatagtca aaatactaac ttttaaatca ttagcaaaat ttcttatgga
     7621 tggcattgca tccccttctt tcagttcttc agtaaatatt gcttctttaa tttgctcttt
     7681 aatttgttgg tataatggct tatctgatgt gttagaaatg agcactttca ttttttatca
     7741 cccccttttt atcactttca gtgtgatgtt atcatataca cactatacac ttagagcact
     7801 tttttgtcaa tataaaagat gtaaaatatt actatgacaa aataaatatg aatgaaatat
     7861 tagagattgt taatagccat aaacactttt taaaactaca taagatagga tgtattaaat
     7921 atttattaaa agtaagaatt gaatatataa aagaaaaagt aatcctgtat gaatgatttg
     7981 tttaatgata aattaagtta taaactattt ataaaaaaca taaaggaata aatccatttc
     8041 taattacaaa catactaaaa aatttagata ataataaata aatagtaata aatgggatga
     8101 atttataaga atttggatag aggaaagagt tattcaagag tgtagactag aggtaaactc
     8161 tatattaaaa aaatttaaat tgggatatta ttatgtatgg aatatttgca gaagtaacct
     8221 tgctataagt atagaagatt tttttgatta caaaagataa tactctaaaa tgtgaggatt
     8281 tcaacattca atactgtgct ttaaataata tcactccaaa ataccatcca ccttttacta
     8341 taattgaata ccctaattat tttaaaaaag aaaacattag aaacggaaaa ccttgtatat
     8401 ataaaataaa aaatactaac ttctaaaaca cgaatactct ttgataaata aaacaaaaaa
     8461 taattttaat aattaaaatt ggctatctca aactagagat aagtctcttt ctcactttta
     8521 aaacgttgaa aatatattcg atttcgtaca aaaaagagtg ccttagaact aaaaagttca
     8581 ctttgtggca ctcccttttt tctcctcttt ttaatttttt ataaaaattc ttcaaaaata
     8641 ttattaaata ccctatatta tacataaatc aaattaaagt tgcattttag caaataaaaa
     8701 caaattataa ctattagcat agcaaataaa aaatctgcta tcttataaaa tcaaaattat
     8761 aattataaaa agaaaatatt cttataacaa attaactaat ataaaattat tttataaatt
     8821 aatactactt tttagcttct cctataaatt aataccactt ttttaactat gaaacctatc
     8881 tacaacattg gtaccataag accagctacc acaatagaaa tcatacaagc cataccacca
     8941 actaccagct taggcataat cttagacatc aaatatgctc tttcttcttc cgtttctcca
     9001 acagcattac acacttcatt tgaaattaaa tatgtagttg ggaatcccaa catctgacaa
     9061 aaccctactc caaaagcaag aggtttagaa ccgataattt tccaacctgg taacaccttc
     9121 atcataagga atataccaat gatagaagct gcaaacatac ccacaattgg tacaaaaaga
     9181 ctaattaaat ctgtcaaaga aacttttgct aaagaaggaa taacagctgc aaacacaacc
     9241 atgttgataa atcccttgat ttttcctttt tccaaaatgt cttttggcac aataccaata
     9301 cgtgttacaa taacaccaac aacaagacat acaatagaat aatttacatg aataatctct
     9361 gaaagttcat accctagata agagaataat gctataaaaa atatacaaac atttgtagaa
     9421 taatatttat cgtatttttc acagaaagct tgttttactt ttgtgcctgt attctctgct
     9481 tctttcttat cagtatcaac atttgctaaa tcaatgcctt ttgattttgc ttcatgatat
     9541 tctactaaaa gacggtttgc ctcttgtaac gctgctcttg aagctattgg tgtaccaata
     9601 aacttctgaa ttgcaaatac aactgttgct gtagctgctg caagtgtcag tcctttttct
     9661 agtgctgctt gtgtcataat agtagtagca ggtaaagctc catttataac tggtatgctt
     9721 gtcaatactg ttgattttcc aaacaaagga atacataatg agacacagac tataactgct
     9781 acaattccaa gccatgctgt caatactgtt ctccattcgt ctataaattg tttaacattc
     9841 aacattgttc ccatactaaa cataatcatt ggaacactcc aactagctgc tgcggtcata
     9901 cctgcttttt caataatatc agctggtatt gcttttgtga caaatagaat caaaaataaa
     9961 agtagtgtaa caaatacagc tgatactttt gcttttgttt tgcttgcaat taaatcccca
    10021 atagcaaata aaactaatac gacactaaac cataataaca tatcactcat agaataaccc
    10081 ccttattata aatatctttc aaaccaatct acaatagcat ttgctcttgc aattctatta
    10141 gatggtcttc catttctaga caatccatgt gtatctccat gaaacaaata catttctgta
    10201 tctacaccaa gctgttttaa tgctgtaaac atttggaaag tatcccccat aaaacatcta
    10261 taatccctat cactatgtaa gaacaatgtt ggtgtcttaa catttttcac atatttaata
    10321 ggtgaatgat tccaaagaac ctcctcatga ttccaaggcg tcttatctcc acaagtctgc
    10381 ttcataattc tatccccaaa gtcaataatt aaagaagctg taatcaagtt actaatacct
    10441 ctttgagaaa ctgctgcttt gaaacggtct gtatgaccaa ttatccagtt tgtcatataa
    10501 ccaccgtaac ttccccctgc aacccctaag cggtctgcat caatcccagg atatttttca
    10561 atacaagtat ctacaaattc cataagatgc tcatagtcaa cataccccca tctttctacc
    10621 atttctaaga atttttcacc ataaccgtca ccaccatgag ggttacaata aatcacatac
    10681 attccctttg atgttacaca ctgcttcaag tgtgtaaaca tatagccata tgcccattta
    10741 ggcccaccat gaatgaacaa tacagcagga tatttttcgc cttctttagc atctataggt
    10801 ggtataacaa aaccatcaac ttcttcttta ttttttgcta caaacgtcaa tttttcaggt
    10861 aatgaaacct tatgagtatc taaatatggt ttagaaaaag cagtcatttt tttcattgta
    10921 cctgtagtca tatcaatgct atatatttct gctaactcat aatctttcaa cattactgtg
    10981 tacatggtgt tgtcgtaaat atcaaaagat gtaaaagcat ctacttttgt gatgatacgt
    11041 atattacctg tctttgtatc aaactcatta aattctgtca tagttctgca aattcttgca
    11101 ccaataaatt tgtctcctat aactttatta ctacgtccac caccatatac tgcatctaca
    11161 tccagcaatg tagcatacat accatctgga ctagcaatca tatttatttc attagtactc
    11221 aaatcaagta taaaaatatc atgattctgt gtgatagaac ggtctaatgt tgaagcttgc
    11281 aacattactt tatctttgcc aatatatttt gccaaactta tttggtactt tccttgtggt
    11341 acaagttctt tggtttcacc actgtctata tgatactccc aaagtccaga aaaaggagtt
    11401 gcacaatctt cataaatctg accagtatat aaaattctgt ttttatcaga agaaacgtct
    11461 acacctgcta cttcacaaaa ttttggtgta atggcttttg ttatattttc accttcttga
    11521 tataattcta atgttacacg tcttctttga gaaaaatttt ctccatctct tataaaaggt
    11581 ttatctgtaa atgtccaata atctacaccc tctactgccc tatctggttc tgatttttta
    11641 gaatccgttg gattttttgt tgtcagtaac cacaatttat ttccaagtga tacaggtacg
    11701 ctacaagctt ttggaataga aaatgctttt tttgcttctc ctccttctat agaaatagtg
    11761 taaaaatctg ttgtacctga ttttggctta tttcttttag atgtaaaaag gagttctgta
    11821 ttagaaatcc aactaaatga tgttacatct tttgtttgag tcatatgtat ttgtttttct
    11881 gttttgatgt ccatcacata taagtcatgt tcatagcagt ctttttcctc gttgatattt
    11941 gatataatat aagcaatatg ctttccatca ggtgaacaag aaatatcatg agggaatata
    12001 tattcaaaaa tatctgaatt ttcaatcttt attttttcca tttttatatt ctcctttcta
    12061 ctgtcctatg ttatcttttt ttatatatac tgaaaaccaa tctagcattt caactaatct
    12121 ttgtagcttt tgttttggtc taccagaacg agacagttca tggttttctc cttcaaatag
    12181 acaaagtttt gtaggacacc catgaatttt caatgctgaa aacatctgga atgcttcttg
    12241 catatagcat ctatagtctt tttcaccatg taaaaataaa gtcggtgtaa cacatttatc
    12301 agcataagca agaggtgact gtttccattg aagctcgtgg ttttccatat gagaagcacc
    12361 tgtttgtcct ttgcagaaat aataaccaat gtctgttgta ccctccatgc cactccagtt
    12421 tgcaatagaa cgctgagcac aagcaacagc aaaacggttt gtatgaccaa ttatccaatt
    12481 cgtcataaat ccaccataac ttccaccttc aacagccaat tttgttttat caatgtctgt
    12541 atatttttca agtacagtat ctgtaaattc cataaaatct atataatcaa tatctccaaa
    12601 ctgtttccta atatctgcaa aagcattgcc acgaccttca gacccccttg ggttacagaa
    12661 gaatacaaag taacctgcac ttgtaaatac ctgtatctca tgcataaaat atggtccata
    12721 tgtcaatctt ggtcctccat gaatcgccaa tacagctgga tattttttcc ccacctcata
    12781 atctactggt ttatatacgt atccttgtat ttcccattta gaacttcctt ttgactctat
    12841 gtattctggc acagaaattt tatattcctc taaaatatgg tcatttaaat gtgttttctt
    12901 acacaatgtt ccattctcta ctgtataaac ttctggtaat ccatttccta aacaacccac
    12961 acatacaata ttgccatctt ttacatctat aaaatcaact gaacctgtct gtgttacttt
    13021 ttcctgtttt cctgttggaa tatgtattct ttcaataatg cagtccatat ctactgtaga
    13081 agtgtaataa atatattctc catctacctt tgttgtctga ccagcaccca ttttaacatc
    13141 agatgttact gtatttccaa tacaatgatg ttgatgccct gaaagaagtt tcatttcttt
    13201 cgtatcaata tcaatagtat aaaaatctcc attttctcca tttccataag ataaagcatc
    13261 agttaggttt agtatcactt gattttgata taattcaaaa ccttttataa tatacttatc
    13321 tttttctaat atacagacat ttgtgtttgt gtttgtatca aatacataga ccccattttt
    13381 caaacctttt acatctttaa actcttgccc tatatataag atttttccat cacttatttt
    13441 catacctaca acattaaaca taggtgctgt aattgccttc aactcattta tattactgtc
    13501 gtatacatat aggcgttttc tttttctgct aatatatccc tccccattga cagcataagg
    13561 aatttctttg aaatgttcat aatgtctttt gttataagca tttatttttt tctgttgttc
    13621 ttcttctggc aatccctcta caataggtcg gtcattgtca taagaatcaa taactgcata
    13681 caaacctttt cctataggat tcaatttacc acctttgata gggagtctaa aaagttcttc
    13741 tgcttctcca ccatttaaag ataaagcata gtaactcatg cattgcttac cgtttttgac
    13801 agcttctttt actttttcat ttcgcaaagc agggaacaag atagtatttt catcttccca
    13861 ggcatatgct ccaacagagt ttatagacgt tatttgtttt aattcgccag ttttattgtt
    13921 taatatataa acttggcttg tgtaaccatt ctctttgata tcagcttttt ttacaataaa
    13981 tgctgtatat cctccatctg gtgatatttg gcaatcactt ataaattgat attccaaaaa
    14041 tgtttcactc gtaaatagct ttttcatagt attttctatc ctttctttag taattttttt
    14101 caaactctct accacctctt tcatttttta ttttaaattt aaaataaaaa attttaaatt
    14161 taaaataaaa aattttataa aggttatttt tttaggaagc cttctggctt ccttgtaata
    14221 tttgtaattt aaagtatatt taatccatgc ttaattcatg cttaattcat acttaatcta
    14281 tacttaatct atacttaatc tatatttaat tcatacttag acttatttac actatattta
    14341 gattatattt attagattat atttagatta tgtttaaact ataattagat tatacttaga
    14401 ctacatttag caacttaata gataagtata tatagtaact tcaaaactta ttattcatct
    14461 tcacagaatc ttttaatagt tccttgaata tgatttctta gtttatctac tgcttttttt
    14521 gtatctctgt cttcaatgaa ttttaatatt tcaaaatggt cttgtactgt tccttcataa
    14581 ttacgatttt ttaatggtcg aattaaatag ttcactacta taaatcttcc ttgtatacca
    14641 acatatacat catttaaaat ctcattattt gccccttcta caatggcttt atgtaattcc
    14701 aaatctgcta gtgcaagttc tacagatttt tcttgacgat agtactcctc gcatttatca
    14761 gagtatcttt ttattacatc tagcttttgt tgaaaaacct cttcctcttc ttgcatcatt
    14821 ttttctaaag catatgtttc aatcagtgtt ctcgtttcac aaacttgttt taccatatca
    14881 tattcatact ctggtacgta cactttacca tttgtcataa tagtaagcac tccatcttta
    14941 tttagcattt ttactgcttg tacaactggt gttctgctta cttcaaattt ttcacaaagg
    15001 tcatttagta atattttttg acctacagag tattcaccac gaataagact ttcaataatc
    15061 ttatgatata aaatttcatc taaatttgac tttactttca ctactctcac cttatttcta
    15121 ttcttttgcc atattatatt gaattactgg actttaatta cttttagtaa tatttttaaa
    15181 gtagaaattc ttatttgata gtacttttgt actacaattt aaattattgt actgtaaatc
    15241 atgctcttat actatagatt aattcattgt attgcaaatc aagttattgt actatagatt
    15301 aaatcgttgt acttcaaatt aaac
//
LOCUS       DAEPIN010000081        15239 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.081, whole genome shotgun sequence.
ACCESSION   DAEPIN010000081 DAEPIN010000000
VERSION     DAEPIN010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15239
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.081"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            192..2090
                     /locus_tag="KR250_003109"
     CDS             192..2090
                     /locus_tag="KR250_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HBH0069003.1"
                     /translation="MDSLLYVIEKDKHTNEELREILKSNKNIRFVSLMGVDLGGNATD
                     EKIPVELFLEDIDKFLESAIQTDGSSVELYNIATLNNAKVDLMPDKSCHWYVDYNMEY
                     IDEEVGLPVGTLKIPAFLIHDNKKVCSRGVLQKADKYFKKSMYEIFREYPHVINNIGI
                     DSVDDIEEIMLTAATELEFWVNTPEDKADLEKLYVSQSLKEQYWKRTHGIIRTCLEKS
                     LIILQKLGVNPEMAHKEVGGIQSSISIDGRTNHAMEQLEVSWKFSTPLQAADNELLVR
                     DVIEDVFTSHGLEVTFKAKPIHGVAGSGGHTHVGVSAKLKDGSIKNLFAPKDLKEDYL
                     SELGYGALMGLLYNYEVLNPIVTASNDGFNRLVPGFEAPVCIVTSLGHSYEIPSRNRS
                     VLVGLIRDMKNPKTVRFELRSPSPLSNTYLVIAGCYQTMLDGIKAAAKSGLSTKELEK
                     ELSKNVGEESFYLEKDRAYRDENDVFEHYSLEERNARFGIPPATVYENMKNLEIYASK
                     LKSLKQGDVFTDSIIESFKIGAIDKWQKKLKTRIIEAGIQKIRSIVKIHTKENMDALD
                     EVVWNSISDLKFNIMKDTLTRESLFTRVREAIENKDYQTASDLQIELKRSMEEIQQLY
                     MQYKKNIY"
     gene            complement(2218..3339)
                     /locus_tag="KR250_003110"
     CDS             complement(2218..3339)
                     /locus_tag="KR250_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosyldiacylglycerol synthase"
                     /protein_id="HBH0069004.1"
                     /translation="MKDDKTILILTAQFGAGHISAAKAVKECIIEKYSNYNVVIQNFI
                     NASIPMMNKPMVKLYENNTKYTPGLYNYYYYFKKSFDSRHDLSHKLYTPKLSEYIADI
                     NPDLIISTFPLAAACVNNFKIKNPDINIPTLTVITDVVDSMEWVFENTDLYFVPSPEI
                     KNRFFQKGINPDSIKVTGVPVDKRFQIESKELCCDKYRLLLLGGGRGLFDIDEDFMHW
                     IDEFIEEHSNSIEITIVTGKNKKLYDNLTHKKPLKNIKVLGFVNDMYNLIRECDLMLT
                     KPGGATIFEAIQSQTPVLVKMPKVGQEIENAKFIIDKGLGMIYSDDLDLKNIFYKLVS
                     NEFDSIINFMKKNLKEFKTVIHPEKIADYISELIDNHYS"
     gene            3538..3870
                     /locus_tag="KR250_003111"
     CDS             3538..3870
                     /locus_tag="KR250_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBH0069005.1"
                     /translation="MNTQFRKGVLEICVLALISKKDMYGYEIVHNISKVIDVNEGTIY
                     PILRRLTKDLYFETYILESNEGPARKYYRITSLGKENLSNLMEEWREFVKAVEILISE
                     DEGGNELE"
     gene            3857..4915
                     /locus_tag="KR250_003112"
     CDS             3857..4915
                     /locus_tag="KR250_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HBH0069006.1"
                     /translation="MNLNKTEFLEILKDYLKKDFSESEVNDILRDYEEYFVDGLIEGK
                     SDMEIIASLGSPKSIAKELILQTKEKNIEEEKIINTDVLNDKFSKFKSDMKKGYSILK
                     DEAKEKYSKSKENIDGKIMNTDILNGKFSRFKSNVKKDYSILKDEAKEKYNKSKENIN
                     DKLTPSIEEGVGGLSRQVIKGLLVVLSLFLIVPAFAFVCAIVSATIALVCSLIAFVIL
                     IPFVIKFIAMVPDVMMFCIFATIAFVGLEILAWQILLFAIKLGKQLLNNYIHWIKRKN
                     IYIKANEKMEKRKKATLNDEDLNKDINEDSYNNDYYNREEESIKETDEYNDDSINFEA
                     IYKDLEDIENKKGEDKHE"
     gene            4908..5978
                     /locus_tag="KR250_003113"
     CDS             4908..5978
                     /locus_tag="KR250_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HBH0069007.1"
                     /translation="MNKKLAIFAAVLLVIGIIGTTWSGILVMPSLINFGLEKEAEFKK
                     ENKLYQEKVNIDKLDIAVDNINVTIKKSSSEDVRVTTRGNNEFYKYNVTLKDKTLVVK
                     GERKYENKIKKIKNFDQLLNTSINSMFSHDYREIIIYVPNNVDINASSISSHLFVYDN
                     VASNTITYKTSYGGFSRAITENKVNRLENLNLISNNNLHLSTKSILGVKNVNIESESL
                     YISSENEDVFINNIEEYIPENVNIKEKIGRNSNYESEFYLYSDMPIAKFLDIEVPNSK
                     VRLDIPVNKYKFNCDIKSKEAIEEFNNDEEYDNDSYDYEHSEEHSNKYRNTREIKGLL
                     NKNLSNLEKEYTIKINSNSLEL"
     gene            6080..6616
                     /locus_tag="KR250_003114"
     CDS             6080..6616
                     /locus_tag="KR250_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBH0069008.1"
                     /translation="MNKIAVSFLIIATTLLSTACMDYSISAVELVDSKESAVVKKDED
                     AKEETTSKMINSKKTTKIPIEIISKDEKIVKYLQIDEESSLKDKLRLILDTLSNEYFN
                     GLDMEVEVKEKDNLVKINLIEPDKKSRVSWKDDYLNEQNIIYTINNIIKNVIQEEDNS
                     IWIEEVEIYYNGKLIELR"
     gene            6732..7439
                     /locus_tag="KR250_003115"
     CDS             6732..7439
                     /locus_tag="KR250_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic protection ABC transporter
                     ATP-binding protein"
                     /protein_id="HBH0069009.1"
                     /translation="MSNYILQVKGVSKKFKEQMAVDNISLAIKSNSIYGLLGPNGAGK
                     STLLKMITGILNPTSGKIMFEGHRWTRKDLSNIGSLIESPAIYENLTARENLKVHTTL
                     LGLPDSRIEEVLETVDLRDTFKKRSGQFSLGMKQRLGIAIALLNHPKLLILDEPTNGL
                     DPIGIEELRELIRSFPKQGITVILSSHILSEVEQVVDEIGIISNGVMGYQGEVSKEQN
                     LEKLFMKVVAENRKRGQ"
     gene            7441..8157
                     /locus_tag="KR250_003116"
     CDS             7441..8157
                     /locus_tag="KR250_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HBH0069010.1"
                     /translation="MERFIRVEFQKMKGTFGMKLIWIVPVFCILLCGLGGGQNAAFNW
                     WYTTFLPGALTLISSLIVLKDEKSKYRGILPLPIEMKDVWTGKMIYCIICCFIASIIS
                     AIGVVFVGIIYKETISIGQNIIATILIILLFMWQIPLCMFLASKFGIFVTVLINMFFT
                     IVGVAVFSTTEMWYMFPYSIPSRLMAVVLHILPNGLPVPEGSEFLNNAVILPGVLISI
                     VLFVLFALATRVWFNNREVK"
     gene            8159..8917
                     /locus_tag="KR250_003117"
     CDS             8159..8917
                     /locus_tag="KR250_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutG family
                     permease subunit"
                     /protein_id="HBH0069011.1"
                     /translation="MRQLIRIFCSDLIKLKRNFIILMHICIALMGMGLFLWYYKVSGA
                     DNILKIVAYLQVIAIAFPLLSGVVCSMFVEQEYYAGNYKHMLTTSNPKYLTLISKYII
                     LVCLGFVATLVSVLGFKFGISKAYFASSFYMISILILIGCNLFEYILHLFLSLRFGKG
                     TSIGVGIVEMLLSALLLTGLGSGIWQYFPCAWGVRFITIWSSFSSSKTVEYIKVESIK
                     AYQSTGLMCGFVTILAFVILCIWFSKWEGKKSEE"
     gene            9052..10440
                     /locus_tag="KR250_003118"
     CDS             9052..10440
                     /locus_tag="KR250_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase"
                     /protein_id="HBH0069012.1"
                     /translation="MLGGKKSNNKKVEKLRTIFIKYLSLFFIMTISIVLLLMLSFSVL
                     LSSGVILPANYAEKQFNKYKEQIISSEKVTEDIIPSIYEYGVYTLDGSLISGTFNKKE
                     SKEVWNLMRDIEERHAYSESYIKFFKKDEVFIIKYKIVSEYSSPILRAYLPKPETLGM
                     IIFSIIFFIEIVILSKVFGKKFNIEMELLKNTTEKIEQQDLDFVVESSKIYEINNVLF
                     SMDKMKLALKDSLEKQWMLEENRKEQISALAHDIKTPLTIIHGNTDLLIEINENPELL
                     EYMEYISKGVTQIEKYINTLIDISKTENGYILNKEAISMSEFIKDVLVQIEALASTKN
                     LDVEFYKQDNLPKNITIDRELLFRAIMNIISNAIDYSPSQSKLYISVSVRNKYLKFVV
                     TDCGSGFSKADLSKATEQFYTGDLSRNSKSHYGMGLYIVNNIVQKHNGILYIENSVKT
                     GGAMVTIEIPIV"
     gene            complement(10516..11337)
                     /locus_tag="KR250_003119"
     CDS             complement(10516..11337)
                     /locus_tag="KR250_003119"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine kinase"
                     /protein_id="HBH0069013.1"
                     /translation="MQKKVAAINDLSGIGKCSLSVAIPILSALKVQCCPFPTAILSSQ
                     TGYPEFTFLDFTDEMVKYSNVWKNLKVNFDSIYSGFLGSKHQIEIVANFINDYPNAFI
                     VVDPVMGDNGVMYPIFTEEMRHEIKELVKHSDLTTPNLTEACFLTGNDYTKSDYNRDE
                     LIYIAKSVSDLGPSKVVITGILEDDNILNLAYDRDNDHVFFTSVKYNNCSYSGTGDIF
                     TSILCGMLVNKHDLGVAVNTATDFIYKTINYTSQFDTDRNDGVMFENFLSDLTNI"
     gene            11573..12742
                     /locus_tag="KR250_003120"
     CDS             11573..12742
                     /locus_tag="KR250_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase domain-containing protein"
                     /protein_id="HBH0069014.1"
                     /translation="MGGVALKILKNKNKYIKGIMAFVFLSLATTAGVFLINDLSMKNM
                     EEKVMESANVEGDDVKDQYKLLLENLFDYRNKAILEKNENILKGLYETDKKFGLWAYE
                     NEVKKMKYLENWSSKQGVKFKDIKTRVKIKKIKEKEKDLYGIICSVSTEYKYSYENQK
                     DKENAFRIGTYHYLNVKIIDNQYVITKEWYTDPFADSLNLENIKSDDIRNYIGQQDGV
                     ELQLTDEQKKAIDYAHKYCGAAADEEHGMKFNPNYRDYNPEGGDCANFASQIMFESGR
                     FKKNSIWNYSERAGTKAWVNAQAFKNYALYSGRGSLIAKGSYEEVYKEAYNLRPGDFV
                     GYEKKGRITHVSTVTGLDSRGYPLVTCHNTDRMLVPWDLGWSDKSIRFHIIKINY"
     gene            12925..13125
                     /locus_tag="KR250_003121"
     CDS             12925..13125
                     /locus_tag="KR250_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBH0069015.1"
                     /translation="MKTGVVKWFNNEKGFGFISVEGEGDVFVHFSAITGDGYKTLEEG
                     QSVEFEVVDGAKGPQAANVVRL"
     gene            13546..13974
                     /locus_tag="KR250_003122"
     CDS             13546..13974
                     /locus_tag="KR250_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBH0069016.1"
                     /translation="MNILFFLTPKSEVAYIYEDYTIRQALEKMEYHKYSAIPIISKDG
                     KYVGTITEGDFLWTLKNDLNLDLKGLEDVPVTDINRKMDNSPVSINADIEDLVIKSLN
                     QNFIPVIDDQDTFIGIIKRRDVIGYCYEIIRGYKNLANDN"
     gene            complement(14117..14872)
                     /locus_tag="KR250_003123"
     CDS             complement(14117..14872)
                     /locus_tag="KR250_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBH0069017.1"
                     /translation="MEKKVLATVGEKEITNIDIENALKSLDPYQAMQFKTEEGKKHLL
                     NDLVNQELFFLDAKEEKLDEEEIFKLEMKKIEENVLKQFAINKVLSSVNVTEDEKVKF
                     FEANKSSFSKPESATAKHILVDSDEKAKEILAQIKSEEISFEDAALKHSSCPSKDMGG
                     DLGTFGRGQMVPEFEEAVFSMAKGEVSEPVKTQFGYHIIKLEDLQESTESTFDEVKAE
                     VEKSLLYQKQNEVYGNKINALNAKYGNLVKYND"
BASE COUNT         5983 a         1666 c         2482 g         5108 t
ORIGIN      
        1 tattgtatgt ataatatttt ttccaataaa aaatattgcg aactttttat acttgatata
       61 taataatata aataaaaaca cgaataaaac acgaataatt tgcaagaaaa attcataaat
      121 gtaaggaaaa tgaaatttat cttgtattta acaaaataaa atatacataa aaattatggg
      181 agagtggaaa aatggatagt ctattatacg taatagagaa agataaacac actaatgaag
      241 aattaagaga aatcctaaaa agcaataaaa atataagatt tgtttcctta atgggggttg
      301 atttaggagg aaatgcaaca gatgaaaaga ttcctgtaga attatttttg gaggatatag
      361 ataaattttt ggaatcagct atacagactg atggttcaag tgttgagctt tataatatag
      421 ctacattaaa taatgcgaaa gtagatttaa tgccagataa aagttgtcat tggtatgtag
      481 attacaatat ggaatatata gacgaagaag taggtctacc tgttggaaca ttaaagatac
      541 ctgcattttt aatacatgat aataaaaaag tatgttctag aggagtactt caaaaagcag
      601 ataagtactt taaaaagtca atgtatgaaa tatttagaga atatccacat gtaataaata
      661 acataggaat agattcagta gatgatatag aggaaataat gttaacagca gctacagaac
      721 ttgagttctg ggttaatact cctgaggata aagctgactt agagaaatta tatgtatcac
      781 aaagcctaaa agagcaatac tggaaaagaa ctcatggtat tataagaact tgtttagaga
      841 aaagtttaat tatacttcaa aagctaggtg taaaccctga aatggctcac aaagaggtcg
      901 gaggaataca aagttctatt agtatagatg gaagaacaaa ccatgctatg gagcaattag
      961 aggtttcttg gaagttttct acaccattac aagcagcaga caatgaatta ttagtaagag
     1021 atgttataga ggatgtattt acatcacatg gactagaagt tacatttaaa gcaaaaccaa
     1081 ttcatggagt agcaggaagt ggtggacata cacatgtagg agtaagtgcg aagttaaagg
     1141 atggaagtat aaaaaatcta tttgctccaa aagatttaaa agaagattat ctaagtgaac
     1201 taggatatgg ggctcttatg ggacttcttt acaattatga agtattaaac ccaattgtaa
     1261 cagcttctaa tgatggattt aatagattag ttcctggatt tgaagctcct gtatgtatag
     1321 ttacatcttt agggcatagt tatgagattc catcaagaaa taggtcagtg cttgttggat
     1381 taataagaga tatgaaaaat ccaaagactg taagatttga attaaggtca ccaagccctc
     1441 tatctaacac gtatttagta atagcaggat gttatcaaac aatgttggat ggtataaaag
     1501 cagcagctaa gagtggtctt agtacaaaag aacttgaaaa agagttatct aaaaatgtag
     1561 gtgaagaaag tttctattta gaaaaagata gagcatatag agatgaaaat gatgtatttg
     1621 aacattatag tttggaagaa agaaatgcaa gatttggaat accaccagct acagtttatg
     1681 aaaatatgaa aaacttagaa atatatgctt caaaattaaa atctttaaaa caaggtgatg
     1741 tatttactga tagtataata gaatcattta agattggagc tatagataag tggcaaaaga
     1801 aattaaaaac tagaataata gaggctggga ttcaaaaaat aagaagtatt gtcaagatac
     1861 acacaaaaga aaatatggat gcgcttgatg aggtagtttg gaactcaata tcggatttaa
     1921 agttcaatat tatgaaggat actttaacta gagaatcact attcactaga gtgagagaag
     1981 ctatagaaaa taaggattac cagactgctt cagatttaca aatagaatta aagagaagta
     2041 tggaagaaat acaacaactg tatatgcagt acaagaaaaa tatatactaa tataacaatt
     2101 tgattttgat atgataaagg caatcaagta ttattaaaca ctatgagtaa attctcaagt
     2161 gtaaaatcat acttgattgc ctttaatttt atgtttgaaa ttttttaata gtacaattta
     2221 actgtaatga ttatctatta attctgatat ataatcagca attttctctg gatgaatgac
     2281 tgttttaaat tcttttaaat tttttttcat aaagtttatt atagaatcaa attcatttga
     2341 tactaattta taaaatatgt ttttaaggtc taggtcatca ctataaatca tgccaagacc
     2401 cttatctatt ataaatttag cgttttcaat ttcttgtcca acttttggca tttttacgag
     2461 tactggagtt tgtgattgaa tagcttcaaa tatagttgct cctccaggct tagttagcat
     2521 taggtcacat tctcttatta gattatacat gtcgtttaca aatccaagta ctttaatatt
     2581 ttttagaggt tttttatgag ttagattgtc atataatttt ttgtttttac cagtaactat
     2641 tgtaatttct atagaattac tatgttcttc aataaattca tctatccaat gcataaaatc
     2701 ctcatctata tcaaatagac ctcttcctcc acctagaagt aatagtctat atttatcaca
     2761 acatagttct ttactttcaa tttgaaatct cttatcaaca ggaactccag taacctttat
     2821 ggagtcagga tttattcctt tctgaaaaaa tctatttttt atttcaggag aaggaacaaa
     2881 atacaaatct gtattttcaa aaacccattc catactatct acaacatctg ttataactgt
     2941 aagtgtaggt atatttatat caggattttt tatcttaaag ttatttacac aagcagcagc
     3001 tagtggaaat gtagagataa tcaaatctgg atttatatca gcaatatact cagaaagttt
     3061 aggtgtatat aatttatgag ataaatcatg tcttgaatca aacgatttct taaagtaata
     3121 atagtaatta tataatccag gagtatattt tgtattgttt tcataaagtt ttaccatagg
     3181 cttattcatc atgggaatac ttgcatttat aaagttttgt ataacaacat tatagttact
     3241 atatttttca ataatacatt ctttgactgc ttttgcagca cttatatgtc cagcaccaaa
     3301 ttgagcagtt aggattaata ttgttttatc atctttcatt ttagccaatt tatcaccact
     3361 ttcataaaat atatctgatt agagtattat acatttatag aaggatttaa tcaacaagaa
     3421 tatttaaaaa taactattgt gcagaacata gtactgtgtt attatgagga tataagaagt
     3481 tttaacatag taccttgtaa aacacaatac tgtgtattta aagaaaggat ggagtatatg
     3541 aacacacaat ttagaaaggg tgttttagaa atttgtgtac ttgctctcat atctaagaaa
     3601 gatatgtatg gatatgagat agtacataac atctctaaag taatagatgt aaatgaagga
     3661 acaatatatc ctattctaag aagacttaca aaagatttgt atttcgaaac atatatacta
     3721 gaatctaatg aaggtcctgc tagaaaatac tatagaataa cttcactagg aaaagagaat
     3781 ttatccaatc tgatggaaga gtggagagaa tttgtaaagg ctgttgagat tttaataagt
     3841 gaagatgagg gaggtaatga acttgaataa aactgagttt cttgaaattt taaaggatta
     3901 tctaaaaaaa gatttttctg agagcgaagt taatgatata ttgagagatt atgaagagta
     3961 ttttgtagat ggtcttatag agggaaagag tgatatggag ataatagcat cacttggctc
     4021 accaaaatct atagcaaaag aacttatctt acaaactaaa gaaaaaaata ttgaagagga
     4081 aaaaataata aatacagatg ttttgaatga taagtttagt aaatttaaat cagatatgaa
     4141 aaaaggttac tctatcttaa aagatgaagc aaaagaaaag tatagtaaat caaaagaaaa
     4201 tatagatgga aaaataatga atacagacat attaaatggt aaatttagta gatttaaatc
     4261 aaatgtaaaa aaggattatt ctatcttgaa agatgaagct aaggaaaagt acaataaatc
     4321 aaaagaaaat ataaatgata aacttacacc aagtatagaa gaaggtgtag gaggattatc
     4381 cagacaagtt ataaaggggt tattagtagt gttatcttta tttctaattg ttccagcatt
     4441 tgcgtttgta tgtgctattg tatctgctac tattgcatta gtatgctcat taattgcttt
     4501 tgttatatta attccatttg tgattaaatt tatagcaatg gttccagatg ttatgatgtt
     4561 ttgtattttc gcaaccatag catttgtagg gcttgagata cttgcatggc aaattttatt
     4621 atttgccata aaactaggta aacaattact aaataattat atacattgga taaagagaaa
     4681 aaatatttat ataaaagcaa atgaaaagat ggaaaaaaga aaaaaagcta ctttaaatga
     4741 tgaagatttg aataaagaca ttaatgaaga ttcatataat aatgattact acaatagaga
     4801 agaagaaagc ataaaagaaa ctgatgaata taatgatgat agtataaatt ttgaggcgat
     4861 atataaggac cttgaagata tagaaaataa gaaaggagaa gataaacatg aataaaaaac
     4921 ttgctatatt tgctgcggtt ttattggtaa taggtataat agggacaaca tggtcaggga
     4981 ttttagttat gccaagccta ataaactttg gtttagaaaa ggaagctgaa tttaaaaaag
     5041 aaaataaact ttatcaagaa aaagtaaata ttgacaagtt agatatagct gttgacaata
     5101 taaacgttac aataaagaaa agttcaagtg aagatgtgcg tgtaacaaca agaggaaata
     5161 atgagtttta caaatacaat gtaactttaa aagataagac tttagttgta aaaggtgaaa
     5221 gaaaatatga aaataaaatt aaaaagatta aaaatttcga ccaattatta aatacatcta
     5281 taaatagtat gttttcacat gattatagag aaattataat atatgtacca aataatgtag
     5341 acataaatgc atcaagtata agctcacact tatttgtata cgataatgta gcttcaaata
     5401 ctataacata taaaacttct tatggaggtt ttagtagagc tataacagag aacaaagtaa
     5461 atagattaga aaacctaaac ttaatatcta ataacaatct tcacttgtca acaaaatcaa
     5521 ttttaggcgt taaaaatgta aacatagaat cagagtcatt gtatatatct tcagagaatg
     5581 aagatgtatt tatcaataat atagaagagt atatacctga aaatgtaaat ataaaagaga
     5641 aaataggtag aaattcaaat tatgaaagtg aattttactt gtactctgat atgccaattg
     5701 ctaagttcct ggacatagaa gttccaaatt ctaaggtaag acttgatata ccagtaaata
     5761 agtataaatt taattgtgat ataaagtcaa aagaagcaat tgaagaattt aataatgatg
     5821 aagaatatga caatgattca tatgattatg aacatagtga agaacatagc aacaagtata
     5881 gaaatacgag agaaataaaa gggcttttga ataaaaatct gtcgaattta gagaaagaat
     5941 atactataaa aataaattca aatagcttag aattgtaaat aatttactat aataatttat
     6001 aaattgattt aaaagtataa aaatataatc tatactatgg tataataagt atcgaaagag
     6061 aattaggaga gggatactta tgaataaaat agcagtaagt tttttaataa ttgctactac
     6121 tttattgagt acggcatgta tggattatag catcagtgca gttgagctag ttgattctaa
     6181 agaatcagca gttgtaaaga aggatgaaga tgcaaaagaa gaaacaacta gtaaaatgat
     6241 taattctaaa aaaactacta aaatacctat agagataatt tcaaaggatg agaaaatagt
     6301 aaagtactta cagattgatg aagaaagttc acttaaggat aaactgcgat taatcttaga
     6361 tacattatca aatgagtatt ttaatggttt agacatggaa gtagaagtta aggaaaaaga
     6421 taatcttgtt aaaataaact tgatagaacc agataaaaaa agtagagttt cttggaaaga
     6481 tgattatttg aatgaacaaa atataatata tactataaat aatattatta aaaatgtcat
     6541 ccaagaagaa gataatagca tttggattga agaagtagag atatactaca atggaaaatt
     6601 aatagaatta agataaaaaa agatgtgttt ttaacacatc tttttttgtt ttttaattct
     6661 tataaaatcc ttatatttat gatttagaat gattcttgta aataagtttg acaaaagaaa
     6721 ggatgaaaat tatgagtaat tatattttgc aggtaaaagg agtttctaaa aaatttaagg
     6781 agcagatggc agttgataac atttcactag caattaaatc taattctata tatggattat
     6841 taggaccaaa tggagctggg aaatctactt tattaaaaat gataactggg attttaaatc
     6901 caacttcagg aaaaataatg tttgagggac atagatggac tagaaaggat ttatcaaata
     6961 ttggttctct tattgagtct cctgctatat atgagaattt aacagcacga gaaaatttaa
     7021 aagtccatac tactttactt ggtcttcctg actctcgcat tgaagaagtt ttagagactg
     7081 tagatttaag agataccttt aaaaagcgtt caggtcaatt ctctctaggc atgaagcaac
     7141 gccttggtat tgctattgca ttattaaatc acccaaaact tttaatctta gatgagccta
     7201 caaatggtct tgaccctatt ggtattgaag aacttagaga attgattcgt tcttttccaa
     7261 aacaaggaat tacagttatt ttatcaagcc atatcttgtc tgaagttgaa caagttgtag
     7321 atgaaatagg aataatttct aatggagtaa tgggttatca aggagaagta tcaaaagaac
     7381 aaaacttaga aaaacttttt atgaaagttg tagctgaaaa tagaaagaga gggcagtaat
     7441 atggaaagat ttattagagt ggaatttcaa aagatgaaag gaacttttgg aatgaagttg
     7501 atatggatag ttccagtgtt ttgtatactt ttatgtggac taggtggagg gcaaaatgct
     7561 gctttcaact ggtggtatac gacatttctt ccaggagcat taacattaat atcttcttta
     7621 atagtattaa aagatgaaaa aagtaaatat cgtggtattt taccattgcc aattgaaatg
     7681 aaagacgttt ggactggaaa aatgatttac tgtattattt gttgttttat agcatcaatt
     7741 atttctgcaa taggtgtagt ttttgttggg ataatctata aagaaactat ttcaataggt
     7801 caaaatatca ttgcaactat attaattatt ttactattta tgtggcagat tcctctttgt
     7861 atgtttttag cttcaaaatt tggtatattt gttacagttc taataaatat gttctttact
     7921 attgttggag ttgctgtttt ctcaactaca gaaatgtggt atatgtttcc atattcgatt
     7981 ccttcaagat taatggctgt tgtattgcat attctgccaa atggtcttcc tgtaccagag
     8041 ggaagtgaat ttttaaataa tgctgttatt ttgccaggag tattaatatc tattgtttta
     8101 tttgtattat ttgctttagc tacaagggtt tggttcaata atagagaggt gaagtaatat
     8161 gagacagtta ataagaatat tttgttctga tttaataaaa ttaaagagaa attttataat
     8221 actaatgcac atttgcattg ctttaatggg gatgggattg tttttatggt attataaggt
     8281 ttcaggagct gataatatac taaagatagt agcatattta caagttatag caatagcttt
     8341 tcctttattg tcaggtgttg tgtgttcaat gtttgttgaa caagaatatt atgcagggaa
     8401 ttataaacat atgttaacaa catctaatcc aaaatatctt actcttataa gtaaatatat
     8461 aatcttagta tgtcttggtt ttgtagcaac attagtgtct gtattaggtt ttaaatttgg
     8521 tatatctaag gcttactttg catcaagctt ttatatgatt tcaattctaa ttttaattgg
     8581 gtgtaattta tttgaataca tattacattt attcttaagt ttaaggttcg gaaaaggaac
     8641 atcaattgga gtaggtatag ttgaaatgtt actttcagca ttattattaa ctggattggg
     8701 ttctggtatt tggcagtatt ttccttgtgc atggggtgtt agatttataa ctatttggtc
     8761 gtctttttcg agttctaaaa cggttgaata tataaaggtt gaatcaataa aagcatatca
     8821 atcaacaggt ttaatgtgtg gttttgtaac aattttagca tttgtaattt tatgtatttg
     8881 gttttctaaa tgggaaggta aaaaatcaga agaatagagt aaaaattgac tgtttattta
     8941 tattattatg ttaaaatata acttagcaat aaaaagatat aaaataatca aataaaaaac
     9001 tggttagata ttaattatga attaattaaa ctattggagg acagggtaca aatgcttgga
     9061 ggtaaaaaat ctaacaacaa aaaagttgaa aagttaagaa ctatttttat aaaatattta
     9121 tctttattct ttattatgac aatttcaatt gtactccttt taatgttatc attttctgta
     9181 ttattatcat caggtgtaat tttaccagct aattatgcag aaaaacaatt taataaatat
     9241 aaagaacaga ttatttcaag tgaaaaagta actgaagata taattccaag tatatatgag
     9301 tatggggttt atactttgga tgggagttta atttcaggga cctttaataa aaaagaatca
     9361 aaagaagtat ggaatttaat gagagatatt gaagaaaggc atgcatattc agaaagttat
     9421 ataaagtttt ttaagaaaga tgaagttttt ataataaaat acaaaatagt atcagagtat
     9481 agttctccaa ttttaagagc atatttacca aaaccagaga cattgggtat gattattttt
     9541 agtataatat tttttattga aattgttata ttatcaaaag tatttggcaa gaaatttaat
     9601 attgaaatgg agcttttaaa aaatactaca gaaaaaatag aacaacagga tttagatttt
     9661 gttgtagaat ctagtaaaat ttatgaaatt aataatgtcc ttttttctat ggataaaatg
     9721 aagttggcgt taaaagattc tttagaaaaa caatggatgc tagaagaaaa tagaaaggaa
     9781 cagatatctg cactggcaca tgatataaaa acaccactaa caattataca tggaaatact
     9841 gacttattaa ttgaaataaa tgaaaatcct gagttattag aatacatgga atatatctca
     9901 aagggtgtca ctcaaataga aaaatatatt aatactttaa tagatatatc aaaaacagag
     9961 aatggctata tattaaataa agaagcaatc agtatgagtg agtttataaa agatgtctta
    10021 gtgcaaatag aagcattagc tagtactaaa aacttagatg tggagtttta taaacaagat
    10081 aatttgccta aaaatataac tatagataga gaactgttat tcagagctat tatgaatata
    10141 atttcaaatg ctatagatta ttcaccaagc cagagtaaac tatacataag tgtatcagta
    10201 cgtaataaat acttaaaatt tgtagttaca gattgtggaa gtggtttttc taaagcagat
    10261 ttgtcaaaag ctacagaaca gttttataca ggagatttga gtagaaattc taaatctcat
    10321 tatggaatgg gactctatat tgtaaataat attgtacaaa aacataatgg gattttatat
    10381 attgaaaatt ctgtgaaaac tggtggtgct atggtaacaa ttgaaatacc aatagtttag
    10441 taaataatat ccattttgta aatacctaaa ttttatgatt tataggtatt tataaaatgg
    10501 atagtttttt tgactttata tattagttaa atctgataaa aagttttcaa acataactcc
    10561 atcatttcta tcagtatcaa attgagatgt gtaatttata gttttatata taaaatctgt
    10621 tgcagtatta accgctactc ccaaatcatg tttatttact aacataccac aaagtataga
    10681 tgtgaatata tctccagttc cactataaga acaattatta tatttgactg atgtaaagaa
    10741 tacatggtca ttatctctat cgtaagccaa atttaatata ttatcatctt ctaatatacc
    10801 agtaataacg actttgctag gtcccaaatc agatacagat tttgcaatat atataagctc
    10861 atctctatta taatcgcttt tggtataatc atttccagtt aagaaacaag cttctgttaa
    10921 attaggagta gttaaatctg aatgtttaac taattcttta atttcatgtc tcatttcttc
    10981 tgtgaatatt ggatacataa ctccattatc acccatcaca gggtcaacta cgataaaagc
    11041 attgggataa tcgtttataa aattagccac tatctcaatt tgatgctttg aacccaaaaa
    11101 accactatat atactatcaa aattgacttt taaatttttc caaacatttg aatattttac
    11161 catttcatcg gtaaagtcta aaaaagtaaa ttcaggataa ccagtttgac ttgaaagtat
    11221 ggccgttgga aatggacaac attgaacctt taatgctgaa agtataggta tagcaaccga
    11281 taatgagcat tttcctattc cagataagtc attaattgct gcgacttttt tttgcataaa
    11341 aaaccctcca ttagtatgta ttaattataa tgataccaaa atatttatat acatatatat
    11401 tactataaaa aaaagaaaaa agtagattat aaaataaaaa attgtaaaaa aaacagtgta
    11461 ttgatacaat taatttttaa tattggacat aataaaatag agaattatta aatatgaaaa
    11521 ttactatatg tgacagaata ttctttgtaa tttgatttgt aaggtgtact atttgggagg
    11581 tgtagctttg aaaatactga aaaataagaa taagtacatc aaaggtataa tggcatttgt
    11641 atttttgtct ttagctacta cagcaggtgt atttttaatc aatgatttaa gtatgaaaaa
    11701 tatggaagaa aaagtcatgg aatctgcaaa tgttgaaggt gatgatgtaa aagaccaata
    11761 taaactattg cttgaaaact tatttgatta cagaaataag gcaatattag agaaaaatga
    11821 aaacatttta aaaggtcttt atgagacgga taaaaaattt ggtctgtggg cttatgaaaa
    11881 tgaagtaaaa aagatgaagt atttagaaaa ttggtctagt aagcaaggtg taaaatttaa
    11941 agacataaag acaagggtta agattaaaaa aataaaagaa aaagaaaaag acctctatgg
    12001 tataatctgt tcagtatcga ctgaatataa gtattcatat gaaaatcaaa aggacaaaga
    12061 aaatgccttt agaataggta catatcatta tctaaatgta aaaattatag ataatcaata
    12121 tgtcataact aaagagtggt atacagaccc ttttgcagac tctttaaatt tagaaaatat
    12181 aaaatctgat gatataagaa attatattgg tcaacaagat ggtgtagagt tacagcttac
    12241 agatgaacaa aaaaaggcta tagattatgc acataaatac tgtggagccg cagctgatga
    12301 agaacatggt atgaaattta atccaaacta tagggattat aatccagaag gtggagattg
    12361 tgcaaacttt gcatcacaga taatgtttga aagtggaagg ttcaaaaaga actcaatttg
    12421 gaattatagc gagagagcag gtacaaaagc atgggtaaat gcacaagctt ttaaaaatta
    12481 tgcactgtat agtggaagag gttcattaat tgctaaaggt tcctatgaag aagtatacaa
    12541 agaagcttat aatttacgtc caggggattt tgttggttat gagaagaaag gcagaataac
    12601 acatgtttcg acagtgacag gattagactc aagagggtat cctttggtaa cttgtcataa
    12661 tactgataga atgttggtgc catgggattt aggttggagt gataagtcca taagattcca
    12721 tataataaaa ataaattatt aaaaaattat aaagaaaaat aaagtgtaaa agataataat
    12781 catttaaaat tataaagtaa aaaaattgat tattaatgta taaaaataat aataattgac
    12841 aaacatagct atatatgata gagtagtaat aaggttacag aaaagagtag ctaaatattt
    12901 gtgttttttt aggaggattt attaatgaaa actggtgtag ttaaatggtt taacaacgaa
    12961 aaaggatttg gattcatatc tgtagaaggt gaaggagatg ttttcgtaca tttctcagct
    13021 ataactggag atggatacaa aacattagaa gaaggacaaa gcgttgagtt cgaagtagta
    13081 gatggagcta aaggtcctca agctgctaac gtagttagat tataattaaa aattcatata
    13141 tattagtgtt ctagtaatat gagagataag ccgctaatca tttagcggtt ttttcagttt
    13201 ttgtaaataa aattaatagc aaaataaatt aaaattatta aaataaaatc attaataatg
    13261 agataaatat gttaaaatta atagtgtaga gtaaaattat tatggctaag tttaagtcaa
    13321 ttaattttta gtaaattttc taaaaagaga ataagtgata ctgatagacc atataaaaat
    13381 attaataaag cataataaga ttaaaatgag caaaggaaaa acgcttgtta cagcgtatag
    13441 tattaagaat acttagaaaa taagaaagga gaaagttact gcaaaaaagt aacattataa
    13501 atattgagta atatttataa gttttcagta acggtgggta gtgtaatgaa catattattc
    13561 tttctaacac caaaaagtga agtagcatat atatatgaag actacactat aaggcaagcc
    13621 ctagaaaaaa tggaatatca caaatactct gcaattccaa taataagtaa ggatggtaaa
    13681 tatgttggaa ctataacaga aggtgatttt ttatggacat taaaaaatga tttaaatctg
    13741 gacttaaagg gattggaaga tgttccagtg acagatataa atagaaaaat ggataattct
    13801 cctgtttcca tcaacgcaga tattgaagat ttagttataa aatctttaaa tcaaaatttc
    13861 attcctgtta tagatgacca agacacattt attgggataa taaaaagaag agatgtaata
    13921 ggttactgtt atgagataat acgtggatat aaaaatttag ctaatgataa ttaaaaatct
    13981 tatttaaact tttaagctga gttctttaca ataaaagaat gataatatga aactgaatat
    14041 ttaaacttta atataaaaag ctgtcttttc taataaatat tgtgaaaaga cagctttttt
    14101 atatatgacc ttatgattaa tcgttgtatt ttacaaggtt tccgtactta gcattaagag
    14161 catttatctt atttccataa acttcatttt gtttttgata taataaactt ttttcaactt
    14221 cagccttaac ttcatcaaat gtagactctg tactttcttg aaggtcttct aattttatta
    14281 tatggtatcc aaattgagtt ttaactggtt cacttacttc acctttagcc atagagaaca
    14341 cagcttcttc aaattcagga accatttgtc ctctaccgaa tgttcctaaa tcaccaccca
    14401 tatctttaga tgggcaagaa gaatgtttaa gagctgcatc ttcaaaagat atttcttctg
    14461 attttatttg agcaagtatt tctttagctt tttcatcact atctactaat atatgtttag
    14521 cagttgcact ttctggttta gagaaagagc tcttattagc ttcaaagaat tttacttttt
    14581 catcttctgt tacattaaca ctagataaaa ctttatttat agcaaattgc tttaatacat
    14641 tttcttctat ctttttcatt tctaatttaa atatttcttc ttcatctaat ttttcttctt
    14701 tagcatctaa gaagaataat tcttgattca ctaaatcatt taataagtgt ttttttcctt
    14761 cttcagtttt aaattgcata gcttgataag ggtctaatga ttttaatgca ttttcaatat
    14821 caatattagt tatctctttt tctccaactg ttgctaaaac ttttttttcc ataatagtta
    14881 actccttcat agtgtatttc attattaata taagactacc atcttaaata atatcagaaa
    14941 ataatgtaga tgtctaatat ttaaagccat attaagtcaa atagaattaa atttagtaga
    15001 acaaagttat tacaattaat aaaagtgagc tcataatacc cactacacct tcgtcatctt
    15061 taaatttaag agtttttact attgaataat aaaggtatat tccagtaaaa ataatcttta
    15121 aatacaagaa aaataaactc tttgggtata ttaaaaagct aaaaagtgta aatataaaag
    15181 caagtagagt acttatcctg taaaagaaat ctatttgtgt aatgtcttta tattttatc
//
LOCUS       DAEPIN010000082        15028 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.082, whole genome shotgun sequence.
ACCESSION   DAEPIN010000082 DAEPIN010000000
VERSION     DAEPIN010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15028
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.082"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            103..1026
                     /locus_tag="KR250_003124"
     CDS             103..1026
                     /locus_tag="KR250_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HBH0069018.1"
                     /translation="MGSGKVGTSLANYFLSKGYCVSGFYGRNQLSLLESTNLTKTKIY
                     SNLKDIIYENDILFITTSDDSIEIIDKKLSKFNLKHKYICHTSGSLKSSILFCSKKAG
                     ALIYSIHPIFAFSNKNTNIKKMKDICFSIEGDNEQDDLVIQNFIDDLGNQFFIRDKDT
                     SSTYHLANVLVSNLVLSLLDIGVSYFIKLGLSEEESLKAISPLVKKNIENILDNGFTK
                     ALTGPVSRGDITPIRKHLSVLDKKDEDIYKILSLNLLKIIALGNDNSLKGIKNITIEN
                     AIENLISKSEKYKEIYKLLGGKNDEKYHTDF"
     gene            1001..1828
                     /gene="panB"
                     /locus_tag="KR250_003125"
     CDS             1001..1828
                     /gene="panB"
                     /locus_tag="KR250_003125"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HBH0069019.1"
                     /translation="MKNTIQTFKNAKYEGKKLSMLTAYDYSIAKIMDECDINGILIGD
                     SLGMVIKGEENTLSVTIDEIIYHTKAVKNGVKNALIVSDMPFLSYHVSIEDAVKNAGR
                     LIKEGGAHAVKLEGGSNVIKQIESIVNAQIPVMGHLGLTPQSVNSFGGFKVQGNTSET
                     ARQLIEDAKLIEKAGAFSIVLEGVPTKIAEMVTNSISIPTIGIGAGINCDGQILVYQD
                     MLGMFGDFVPKFVKQYANIGDIMKDSIKNYILEVNTGTFPQEKHSFSINESELEKLY"
     gene            1847..2695
                     /locus_tag="KR250_003126"
     CDS             1847..2695
                     /locus_tag="KR250_003126"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HBH0069020.1"
                     /translation="MLVKEIKLLRNIIKDWRKHGYSIGLVTTMGFLHEGHQSLIKKAV
                     KENDKVVVSVFVNPTQFGPNEDFNSYPRDIDKDFKYCMDSGATVVFNPSPEEMYLKGN
                     CTTINVSGLTDFLCGAKRPVHFGGVCLVVSKFLNIVTPDKAYFGEKDAQQLAVIKRMV
                     KDLNIDTEIIGCPIIRENDGLAKSSRNTYLSEEERKSALILNKSLSLAKEELVKGNLN
                     PENIKELITAKINSEHLAKIDYVEIVDSETLQPVKQIEHSILVAIAVFIGKTRLIDNF
                     TFELNI"
     gene            3106..3288
                     /locus_tag="KR250_003127"
     CDS             3106..3288
                     /locus_tag="KR250_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069021.1"
                     /translation="MFKDKIDECVHIMTAYIASLKEYYSFIETQIGDFIKKYGEDVVE
                     LCLHRVMILLCECGLA"
     gene            4120..5034
                     /locus_tag="KR250_003128"
     CDS             4120..5034
                     /locus_tag="KR250_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2935 domain-containing protein"
                     /protein_id="HBH0069022.1"
                     /translation="MIDNQKYVILSLELHLFFSRIMKEHALFLEAGFTNKNYNLAMEA
                     DHYKKQFEDLLSYTVSASNGIIRPDILYSEELVTTLTSVAEQKTEEFTGIEINKNITT
                     RELNLQSGVNPQVGQDLVNYVAQLNSDAIRLLDGLINFKERVLDGVLSCTIFTSNYPL
                     LLEHIIHEANLYRYYVVDLENKIDIESKNAKEIELFWDHIMMEHALFMRGLLDPSEGE
                     LINTSNDFAIKFNELIEKTNEMTDSNIKNITEETLNETVEFKDFKEAGASGIEQCKIK
                     SIILPLLADHVLREANHYIRILESYKNM"
     gene            5424..6071
                     /locus_tag="KR250_003129"
     CDS             5424..6071
                     /locus_tag="KR250_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HBH0069023.1"
                     /translation="MIRQTPLEKRYDKLGPHIVTALKKRYFDAYYCKTRNEALQQILD
                     LIPQNHVVSWGGSETLKEMGVQTAVREKGFKVIDRDLAKTPEERTEIMRQALLCDTFL
                     MSSNAISEDGQLFNIDGNGNRVAAMIFGPKNVIVVAGMNKIVKNIDDAIQRTRTIASP
                     AVVQRFQDVNTPCYINGSCIDCTSPESICAYMVTTRISRPAKKIKVILVGENLGL"
     gene            complement(6238..6807)
                     /locus_tag="KR250_003130"
     CDS             complement(6238..6807)
                     /locus_tag="KR250_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBH0069024.1"
                     /translation="MKKFIICIIMFAISVLVLAGCTNDNKIKNEVDSMFNSIKTQDAD
                     TVDKYFPKSGLGNLIYEDKEGFKMYSKNMTWEISDIKNNKDKVTVDLKINNTDMVSIL
                     KKNPPKLGEMMPNPTMKDIDSAKKKEFNITLELVKSGDNYVCKVNQESAMKLLNVITG
                     GGIDYLADYNKDYYKQLDEEQKNYENKNS"
     gene            complement(6812..7711)
                     /locus_tag="KR250_003131"
     CDS             complement(6812..7711)
                     /locus_tag="KR250_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBH0069025.1"
                     /translation="MKLLSKVNNNIRLITQVAFTALTNGYVNGFLEGTIYGGESKKLC
                     VPGLNCYSCPGALGACPIGSLQAVLSSREYKFSFYVVGFLMAFGAFFGRFVCGWLCPF
                     GLVQDLLHKVPFPVKIKKVYGDKYLKYLKYIVLIMFVIILPVAIVNVAGGGNPWFCKW
                     ICPSGTLLGGILLILGNENLRESIGFLFSWKLSILLLILLMSIITYRPFCKYICPLGA
                     IYGFFNPISIYRFKIDKDKCTNCTACQTKCKLDIPIYKDVNSPDCIRCGECIKVCPQN
                     AITTTFSKDKKENNICAKKYRGI"
     gene            complement(7722..7871)
                     /locus_tag="KR250_003132"
     CDS             complement(7722..7871)
                     /locus_tag="KR250_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069026.1"
                     /translation="MSNFIKDNKKGISIIAISIIFIALGTYRGETNTVLTKAIMICLE
                     CIGIG"
     gene            complement(7864..8448)
                     /locus_tag="KR250_003133"
     CDS             complement(7864..8448)
                     /locus_tag="KR250_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HBH0069027.1"
                     /translation="MKRFFIVVLGCICVLGMISGCSKKESKPVEKNKNNSEWFSNLDT
                     EDVDGNKVTKEIFSNKELTLINVWTTWCGPCVGEMPELEALSKEYESNNSNVAIKGLV
                     VEVDKTDMRTGLSNEEKNLVKDIMKKSDATYQQLTVSEELKKTDFKRVMEFPTTYFVD
                     KKGNFVGEKVVGANSKEDWKKIIDERLKMVKANE"
     gene            8837..10162
                     /gene="cdeA"
                     /locus_tag="KR250_003134"
     CDS             8837..10162
                     /gene="cdeA"
                     /locus_tag="KR250_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter CdeA"
                     /protein_id="HBH0069028.1"
                     /translation="MENLFTRKFTTFEFLKFVSPAIISMIFISLYTIIDGIFVSTLVG
                     SDALASINIVLPIINLVCGFGIMMATGGGAIVSIRMGENRQDEANSTFSFIVLFSLIV
                     GILFTVISYFFIKEISILLGATDKLLPYCITYGKVMILCTPFYILKFIFEYFARTDGN
                     SKFSLFLSVIGGVTNIILDYVFIKYFGMGLLGAAVATAIGIILTCVLGIIYFLSNKST
                     LKLRKPKTDFRLIRDTMINGSSEMVTELSTGITTFLFNVVALKLAGENGLAALTIVLY
                     AHFLMTSVYLGFAAGVSPLISYNFGAENSDKLKETFKHSLKFIFISSLLVFIIALVFA
                     PFIVRVFVNPDNTVFKLALQGLKIFAFAFLFVGINIFASGFFTAFHNGKISAIISFSR
                     AFVFIIIGIIILPPMLNMTGLWLTVPFAEVITIFISILFIKKYKCRYKY"
     gene            complement(10257..11051)
                     /locus_tag="KR250_003135"
     CDS             complement(10257..11051)
                     /locus_tag="KR250_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0069029.1"
                     /translation="MNELKPYIKFFNKNTKLYIIIFMIVGIVMNILPVFILKIFGEAS
                     KTNLSTIVNPILTYITILLFVYGVNIANKDFSGALSIRADRKSCIKAIVIDLIILSFV
                     ISILGIVLIFLSKLFIEIITGYSIELSVLLKRDMIWTSISALIDPSTSSMPLTYLNYF
                     VQICINEICIGFIGVLLGAFTYRVRKVTSITILIGLPILMVIYIVNFATKNMHKMLLY
                     LEKIIYSFQNPFILFGTKVGIIVICIIFIILLLRKAPIKEYANDLL"
     gene            complement(11052..11927)
                     /locus_tag="KR250_003136"
     CDS             complement(11052..11927)
                     /locus_tag="KR250_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0069030.1"
                     /translation="MSSSIKIENLSHSFKKQNIFNNMSLNFEKNKIYGLLGKNGAGKT
                     TLLNIMVNQLIQNEGEIEILGKNPKEDIKVLEEVCIVREKEFYSPDFKVGQIFEFSSS
                     FYKSYDKGLEKELCKYFDLNTKKKYKQLSRGMKTILSNIIGICSNSAITIFDEPTIGL
                     DAVNRQEFYNIILDSYIKNPRTIIISTHLIDEIDDLLEHVIILNEGKIIIDEEIDVIK
                     QKAHYITGNKEELEKLECIKNIKPKKAFGNAVAYFYYGDFSENDEKILKNSNIDVGYI
                     GLQDMFVNMTKKEEL"
     gene            complement(11931..12299)
                     /locus_tag="KR250_003137"
     CDS             complement(11931..12299)
                     /locus_tag="KR250_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBH0069031.1"
                     /translation="MKLVLNNEEPIFIQIARAIEDEILSNGIKEEEQVPSTTELSKLY
                     KINPATVLKGINILVDKNILYKKRGIGMFVSDGAKTIIKEARKENFKHNFVKNLLQEA
                     NKLEINREELVDIIINFKED"
     gene            complement(12482..13141)
                     /gene="deoC"
                     /locus_tag="KR250_003138"
     CDS             complement(12482..13141)
                     /gene="deoC"
                     /locus_tag="KR250_003138"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HBH0069032.1"
                     /translation="MKHILKTVDHTILKATTTWEDIKILCDEAVDMSVASVCIPPSYV
                     KRASEYLKGKIKICTVIGFPLGYQTTATKVFEAKDAIENGADEVDMVVNISDIKNKDY
                     DNIGKEIKEIKKAIGDKVLKVIIETCYLDEDEKIKMCEIVTMSGSDFIKTSTGMGTGG
                     ATLEDIKLMKEHVGKNVKIKAAGGVKSISDAEKFIEAGAERLGTSSICKILKNEDTTD
                     Y"
     gene            complement(13177..14364)
                     /gene="murJ"
                     /locus_tag="KR250_003139"
     CDS             complement(13177..14364)
                     /gene="murJ"
                     /locus_tag="KR250_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HBH0069033.1"
                     /translation="MKQEPCDFSHGRFRTGIIFIGITSVMSAFLQIKENFIVVGFGSI
                     PYNIVIIISIMLSTIFGPYILPIGAVVAMVVQLLFYMFFVKKTNYKYLYYLNFKDDSL
                     IKLLALLSPVFIGVAVNQVNSLVDTTLASTLVKGSIPALTYADRLNGFVTGTFTASIV
                     SVMYPMLSKLSAENNQKKFTSSVKSSINMIIISMIPISVASIFFATPVVRIIFERGAF
                     DARATQMTATALIFYAVGMTAFGLRDILGKVFYSLQDTKTPMVNGIISVGVNIVLDLV
                     LIKPMAHGGLALATSSSSIACILLLFLNLKRKVGYFGQDKIIKATLKSVVASLIMGVL
                     SYFTYKFIFGILGVGTFNEFVSLAISVIVGGGIYTLLMTIFKVEEVDMILNIAKRKLH
                     LKK"
     gene            complement(14441..>15028)
                     /locus_tag="KR250_003140"
     CDS             complement(14441..>15028)
                     /locus_tag="KR250_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBH0069034.1"
                     /translation="TNNQIGLDLGIKEFCISSCGEFIENPKYFKKSLNKLAKLQRELS
                     RKTIGSLNRNKARLKVARLQEHIANQRNDFLQKLTTKLIKENDIICIEDLQVKNMIKN
                     HKLSRLISDVSWSEFIRQLEYKANWHGRQIVKVGKFFASSQICNKCGYKNEEVKNLNI
                     REWICPSCNETHDRDINASRNILKEGLRLITIQNK"
BASE COUNT         5392 a         2205 c         1818 g         5613 t
ORIGIN      
        1 tttttaatta ttttgtctta tgtaactcat atcctcagga atgagattag aaattaagat
       61 gttgtatcac aaagtggagg tgatactatt aaaataggct ttattggttc tggaaaagtt
      121 ggaacatctt tggcaaatta ttttttatca aaaggttatt gtgtttcagg cttttatgga
      181 aggaatcaat tatctttatt agaatcaaca aatttaacaa aaacaaagat ttacagcaat
      241 ttgaaagata taatttatga aaacgatata ttatttatta caacatcaga tgattctatt
      301 gaaattatag acaaaaaact ttccaaattt aatttaaaac ataaatacat ttgccacaca
      361 agtggctctt taaaatctag tatattgttc tgttcaaaaa aagcaggtgc attaatttat
      421 tctatccatc ctatatttgc attttccaat aaaaatacca acataaaaaa aatgaaagac
      481 atttgttttt ccatagaagg tgataatgaa caagatgatt tagttattca aaatttcata
      541 gatgatttgg gaaaccaatt ttttataaga gataaggata cttcatccac ttatcattta
      601 gctaatgttt tagtttcaaa tttagtatta tctttactag atataggagt aagttacttt
      661 attaaattag gtttatctga agaggaatct ttaaaagcta tcagtccttt agtaaaaaaa
      721 aatatagaaa atatacttga taatggcttt actaaagctc ttactggtcc tgtttctcgt
      781 ggagatataa caccaataag aaaacatcta tctgtattgg ataaaaaaga tgaggatatt
      841 tacaaaatac tatccttaaa cctactaaaa ataattgcct taggaaatga caattcatta
      901 aaaggtataa aaaatattac tatagaaaat gcaatcgaaa atttaataag taaatctgaa
      961 aaatacaaag aaatttacaa acttttagga ggaaaaaacg atgaaaaata ccatacagac
     1021 ttttaagaat gctaaatatg aaggaaaaaa actatctatg cttactgctt atgactactc
     1081 aattgcaaaa ataatggatg agtgtgatat aaatggtatt ttaataggag attcattagg
     1141 aatggttata aaaggtgaag aaaacactct atctgttacc atagatgaaa ttatatatca
     1201 tacaaaagct gtaaaaaatg gtgtaaaaaa tgccttaatc gtaagtgata tgccttttct
     1261 atcttaccat gtttctatag aagatgcagt taaaaacgcg ggtagactta taaaagaagg
     1321 aggagctcat gctgtaaagt tagaaggtgg ctcaaacgtt ataaagcaaa tagaaagcat
     1381 tgtaaatgct caaatacctg taatggggca tttaggatta actcctcaat cagtaaattc
     1441 atttggtggg tttaaggttc aaggaaatac cagtgaaaca gcaagacaac ttattgaaga
     1501 tgctaaatta atagaaaaag caggtgcatt ctctattgtc cttgaaggag tgcctacgaa
     1561 aatagctgag atggtaacta attctatttc aattcctaca ataggtatag gggcaggaat
     1621 taactgtgat ggacaaattt tagtttatca agatatgttg ggaatgtttg gagattttgt
     1681 tcctaagttt gtaaagcagt atgcaaatat aggagatata atgaaagatt caattaaaaa
     1741 ttacatccta gaagttaata caggtacatt tccacaagaa aaacacagct tttcaataaa
     1801 tgaatctgag ttagaaaaat tatactaata ttaaggatgg tacaaaatgc ttgtaaaaga
     1861 aattaaatta ctaagaaata taatcaaaga ttggagaaaa catggctatt ctatagggct
     1921 tgttacaaca atgggatttt tacatgaagg acaccaaagt cttataaaaa aagctgtaaa
     1981 agaaaatgat aaagtagtag tgagtgtatt tgtaaatcca actcaatttg gaccaaatga
     2041 agattttaat agctatccaa gagatataga taaagatttt aaatattgta tggatagtgg
     2101 agcaacagta gtctttaacc catcaccaga agaaatgtat ttaaaaggta attgtactac
     2161 cattaatgtt tctggactta cagattttct ttgtggagct aaaagacctg ttcattttgg
     2221 aggtgtctgc ctagttgttt caaagtttct aaatatagta acacctgata aagcttattt
     2281 tggagagaag gatgcccaac aattagctgt aattaaaaga atggtcaagg atttaaacat
     2341 agatacagaa ataataggct gtcctataat tcgtgaaaat gatggattag caaaaagttc
     2401 gagaaataca tatctctctg aggaagaacg aaaatctgct ctaatattaa ataagagctt
     2461 aagtttagca aaagaagaat tagtaaaagg caatctaaat ccagagaata taaaagaatt
     2521 aataacagct aaaataaatt ctgaacactt agcaaaaata gactatgttg aaattgtaga
     2581 tagtgagaca ttacaacctg ttaagcaaat agaacattct atacttgtgg caattgctgt
     2641 gtttatagga aaaaccagac taattgataa ttttacattt gagttaaata tatagtctat
     2701 aattttatat aatatatcta aaaaataata gaatacctaa aaagtaataa attacctaaa
     2761 aaataataaa ttgcttagaa aataatagat tacaataact aaaattttaa aattaaattc
     2821 cttaaaagta tgattaatta taattatata agtatattct tattacactt atattaattt
     2881 atattaaata tctactaact caaatattca taattaaact ttaaactgga taaattttct
     2941 tttaagaatg aaagtttatc cagttttgcg attataataa ccagttttac atatccactt
     3001 gtaaaatata tctaagcaat atatcatact atatagattt atgagtgaaa taatatctgc
     3061 tatttactga atatttttat ttttcgtttt ttagggggat tagttgtgtt taaagataag
     3121 attgatgaat gtgttcatat aatgactgca tatattgcta gtttaaaaga atactattca
     3181 ttcatagaaa ctcaaattgg tgattttatt aaaaaatatg gagaagatgt tgtagagtta
     3241 tgtctccata gagtgatgat tctactatgc gaatgtggtt tggcataaaa ttaaatttat
     3301 taaaatgggt aagaagaatc ctatataata ttcttcttac ccattttaat atatcaattt
     3361 catagcttat cgtttaaaat attttacatc cattactaga caactttaca ttctcttttt
     3421 aattctagta tatttaaatc attttagttg ctccttattt attcttatta cttgttttat
     3481 tttataaata aaataattct ttttaataat gttttttgtt gatattccta tcacacatcc
     3541 aattatttat cattgtcttg aaattgtttg catacttaat ttaatccaat tgaaacaccc
     3601 cagcatagaa tcccctgtaa aatagaagtt ggattaaatc ctaacatcta tacagaaaat
     3661 actactccaa gcacaagcaa tataatggaa tatatttatc acttaatttt ttatactttt
     3721 taaatcccat acctaaagca ttaagtgcag ctattagaat taataattgt tgtggtataa
     3781 aattatgctt gcttcatatt tattcatttt ttactataaa agttcttgtt ttacaaaaaa
     3841 catttgtttt gtatcaaaat aatacttgta aattacataa ttctcataaa aatttctagt
     3901 attacttgta ctcttaataa ataatagcca acaagtacct taaatcatta tatctatatc
     3961 taataagaaa cacagtaaga taattttttc gcccataact taataaaaac tgttgatatt
     4021 agtagcaatt gaatttagtt aatttattta taatatacat aaatttaaat attacgaata
     4081 aagtattata aataggatac tttctaaagg gaggaaatta tgatagataa tcaaaaatat
     4141 gttattttat cactagaatt acatttattt ttttcaagaa ttatgaaaga gcatgctctt
     4201 tttttagaag caggattcac aaataaaaat tataatcttg ctatggaagc tgaccactat
     4261 aaaaagcaat ttgaagattt attatcatac actgttagtg ctagtaatgg tataattaga
     4321 cctgatatat tatattcaga agagcttgta actactctca catcagttgc agaacaaaaa
     4381 acagaagagt ttacagggat agaaataaac aaaaacatca ctacaagaga attaaatcta
     4441 caaagtggtg taaacccaca agttggtcaa gacttagtga actatgtagc tcaacttaac
     4501 tctgatgcaa taagattact tgatgggctt attaatttta aagaaagagt cttagatggt
     4561 gtactatcat gtactatatt tacatcaaac taccctctac ttcttgaaca tataatacat
     4621 gaagcaaatt tatatcgtta ttatgtagtt gaccttgaaa ataaaataga tattgagtca
     4681 aaaaacgcta aagaaataga attattctgg gaccatatta tgatggaaca tgctctgttt
     4741 atgagaggat tactagaccc ctcagaaggt gaactaataa atacttcaaa tgattttgct
     4801 ataaaattta atgaattaat tgaaaaaaca aacgaaatga ctgattctaa tatcaagaac
     4861 attacagaag aaactctaaa tgaaactgtt gagtttaaag attttaaaga agcaggagca
     4921 tcaggaatag aacagtgtaa gataaaatct ataatattac cacttttagc agaccatgtt
     4981 ttaagagagg caaatcatta tattagaata ttggagagtt ataaaaacat gtaattaaaa
     5041 attactttta aaataatctt tgcatattta gataaaataa aggtacagtt aactgtacct
     5101 ttatttttta taaacttata aaatttaaac aagttattca taaaataaaa taataatcat
     5161 ctataaccca caactttatt ttatattaaa taaaaagtat tagtgattat gttttatagg
     5221 tatttcttta ttcagttatt tattaaaaac taatagtaaa tcttatacta atcgcatatt
     5281 tatgttataa ctatattgta actattttcg taatcttagg aaaagattac ttataaaatt
     5341 aaccttatac aataatatat cgtataatct taattagcat aatagttaca atattaaaat
     5401 ttaaatatgg gagtgatttt agtatgataa gacaaacacc attagagaaa cgatatgaca
     5461 agctaggtcc tcatattgta acagcactca aaaaacgtta ttttgatgct tattactgta
     5521 aaactagaaa tgaagctttg caacaaattt tagatttaat tcctcaaaat catgtagtat
     5581 catggggagg atctgaaaca cttaaagaaa tgggcgtaca aactgctgtt agagagaaag
     5641 gatttaaagt tattgataga gatttagcaa aaactccaga agaaagaact gaaattatgc
     5701 gtcaagcact tctatgcgat acttttttga tgagtagcaa tgcaattagt gaagatggac
     5761 aactctttaa tattgatgga aatggcaatc gtgtagctgc aatgatattt ggtccaaaaa
     5821 atgttatagt tgtagctggt atgaataaaa ttgtaaaaaa cattgatgat gcaatacaaa
     5881 gaactagaac aattgcatct ccagcagttg ttcagcgttt tcaagatgtc aatacaccat
     5941 gctatattaa tggaagttgt atagattgca caagccctga aagcatttgt gcatacatgg
     6001 ttactacaag aatcagtcgt ccagcaaaaa aaataaaggt aatattggta ggagaaaatc
     6061 ttggtctata aaatatacat acatgtaaat agctctataa ataataaaat catatgaaat
     6121 aacttatttc taaaagtggt agaataaaag cttctacaag actttcattc taccactttt
     6181 atattctttt aatttgtgtt tctatattat taattgctcc gattttcata tatattacta
     6241 actattttta ttttcataat ttttttgttc ttcatcaagc tgtttataat aatctttatt
     6301 atagtcagct agataatcta taccacctcc tgtaatcaca tttaataatt tcattgcaga
     6361 ttcttgatta actttacaaa cataattatc accagattta acaagctcta atgtaatatt
     6421 aaattctttt ttctttgcag aatcaatatc tttcatagtt ggatttggca tcatttctcc
     6481 tagtttagga ggattctttt ttaaaataga aaccatatca gtattgttaa tttttaaatc
     6541 tacagtcact ttatccttat tattctttat atctgaaatt tcccaagtca tatttttaga
     6601 atacatttta aatccttctt tatcttcgta tataagatta cctaaccctg attttgggaa
     6661 atacttatca acagtatctg catcttgtgt ttttattgaa ttaaacatac tatccacttc
     6721 attttttatc ttattatcat tagtacaacc agctaacacc agtactgata ttgcaaacat
     6781 tataatacat ataataaatt tcttcattaa attatatccc cctatacttt tttgcacaaa
     6841 tattattctc ttttttatct tttgaaaaag ttgtagtaat tgcattttgt ggacaaactt
     6901 ttatacattc accacaccta atacagtctg gactatttac atctttatat attggtatat
     6961 caagcttaca ttttgtttga catgctgtac aatttgtaca tttatcttta tcaattttaa
     7021 atctatatat tgaaatagga ttaaaaaaac catagattgc tcctaaaggg catatatact
     7081 tgcaaaatgg cctataagta atgattgaca tcaataatat caatagcaaa atacttaact
     7141 tccagctaaa taaaaatcct attgattctc ttaaattctc atttcctaaa ataagtagta
     7201 ttcctcccaa aagtgttcct gaagggcaaa tccatttaca aaaccaagga tttccacccc
     7261 ctgctacatt tacaatagca actggcaaaa taattacaaa cattattaag actatatact
     7321 ttagatattt tagatattta tcaccataaa ctttttttat ctttactgga aagggtactt
     7381 tatgtagtaa atcttgtaca agtccaaagg gacaaagcca accacaaaca aatctgccaa
     7441 aaaaagcacc aaatgccatt aaaaaaccaa caacataaaa tgagaattta tactctctgc
     7501 ttgataaaac tgcttgtaat gaacctattg gacatgctcc aagtgcacct ggacatgaat
     7561 aacaattcaa tccaggtaca cataattttt tactttctcc tccatatata gttccttcta
     7621 aaaatccatt tacatatcca tttgttaatg ctgtaaatgc aacttgagta ataagtctta
     7681 tattattatt taccttagat aataacttca tccctcttct cctaaccaat accaatacac
     7741 tcaagacaaa tcataattgc tttagttaat actgtatttg tttctccacg atatgtacct
     7801 aatgcaataa atataatact aattgcaata atagaaatgc cttttttatt atctttaata
     7861 aagttactca ttggctttta ccatttttag tctttcatct ataatttttt tccaatcttc
     7921 cttactattt gcacctacaa ccttctcacc cacaaagttt cctttcttgt ctacaaaata
     7981 agttgttgga aactccataa ctcttttaaa atcagttttt ttaagttctt cagatacagt
     8041 caattgttgg taagttgcat ctgacttttt cataatgtct ttaactaaat ttttctcttc
     8101 atttgaaaga ccagttctca tatcagtttt atcaacttct acaactaatc cctttatagc
     8161 tacattggaa ttattactct catattcttt tgataatgct tcaagctctg gcatttcacc
     8221 cacacatggt ccacaccaag tagtccagac attaataaga gtaagttctt tatttgaaaa
     8281 tatctctttt gttaccttat ttccatcaac atcctccgta tctaaattac taaaccattc
     8341 actattattc ttgttttttt ctactggctt agattctttt ttgctacatc cagaaatcat
     8401 acctaaaaca caaatacatc ctaaaactac tatgaaaaac cttttcatat ctcacactcc
     8461 tagaatttat atagtatata aaatcgctgc ttgaaataaa aataacttat cttctatatt
     8521 aaagtaatta tatttaataa ataagagcta atttaaaaaa agatatacag ttaaaatcta
     8581 tttcttttca gacaaaatct tatatatata taatagcttg taaatcttaa gattatatac
     8641 atatatttct aaagattttc ttaagaaaca gttagatttt actaatataa gtttattaat
     8701 tagcattata ctaatttatc ttaatatgat tttttgttga ttttaagctg tttatcgtat
     8761 aaataaaatt tttcttgact atatagttac tatatagctt attatatata gagagtaata
     8821 aaaggaggta aaatttatgg aaaatttatt tacaagaaaa ttcactactt ttgaatttct
     8881 aaaatttgtt tctcctgcaa ttatatccat gatatttata tctttgtata caataataga
     8941 tggcatcttt gtatcgacat tagttggttc tgatgctctt gctagtataa atattgtact
     9001 acctataatt aaccttgttt gtggatttgg aataatgatg gcaactggtg gaggtgctat
     9061 cgtttctata cgtatgggtg aaaatagaca ggatgaagcc aactctacat tttcttttat
     9121 agttttgttt tcattgattg ttgggatttt attcacagta atctcatatt tctttatcaa
     9181 agaaatatct attttgcttg gtgcaacaga taagttatta ccatattgta taacttatgg
     9241 taaagttatg attttatgta caccatttta tattttaaaa tttatatttg agtactttgc
     9301 aagaactgat ggaaattcta aatttagttt atttctatca gtcattggtg gtgtaacaaa
     9361 tataatattg gattatgtat ttattaaata ttttggaatg ggtcttttag gagctgcagt
     9421 tgcaactgct ataggtatta ttttaacttg tgttttaggt attatttact tcttatctaa
     9481 taaatctaca ctaaaattaa gaaaaccaaa aaccgatttt agacttataa gagatactat
     9541 gataaacggt tcttctgaaa tggttacaga attatctaca ggaattacaa cattcttatt
     9601 taatgtagta gctttaaaat tagcaggaga aaatggactt gctgctctta ctatagtatt
     9661 gtatgctcat tttttaatga catcagtcta tctaggattc gctgctggag tgtctccatt
     9721 aataagctat aattttggtg ctgaaaacag tgataaatta aaagaaacat ttaaacattc
     9781 tctaaaattt atatttattt cttctctttt agtgtttatt attgctttag tatttgcacc
     9841 atttattgtt agggtctttg taaatccaga taacacagta tttaaactag ccttacaagg
     9901 attaaaaata tttgcatttg cttttttgtt tgttggtata aatatatttg catcaggatt
     9961 ttttacagca tttcacaatg gaaaaatttc agctattata tcttttagtc gtgcctttgt
    10021 ttttataatc ataggaatca taattcttcc tcctatgtta aacatgactg gattatggct
    10081 tacagttcca tttgctgagg ttataaccat atttatatct attctattta taaaaaaata
    10141 taaatgtaga tataagtatt aaaaattaat acttaaaatc aacatttatt aaaactaata
    10201 aacctgcttt aaaatacaaa tttacaaatt tgatttttaa gcaggtttta ttttagttat
    10261 aataagtcat ttgcatactc tttaattggt gcttttctaa gtaataaaat tataaatatt
    10321 atgcaaataa ctataattcc tacctttgtt ccaaataaaa taaaaggatt ctgaaatgaa
    10381 taaataattt tttctaaata caatagcatt ttatgcatat ttttagttgc aaaattaact
    10441 atataaatta ccattaatat cggtaatcct atcaatatag taatactagt aacttttcta
    10501 actctatatg taaaagctcc aagtaacact cctataaaac ctatacatat ttcatttata
    10561 catatttgta caaaataatt taaataagtt agtggcatac ttgatgttga aggatctatc
    10621 agtgctgata tagatgtcca aatcatatct ctctttagta aaactgatag ttctatactg
    10681 taacctgtta taatttctat aaataacttc gataaaaaaa tcaaaactat acctaaaata
    10741 gaaatcacaa agcttaaaat aattaaatct atgacaatag cttttataca actttttcta
    10801 tctgctctta tacttaaagc tcctgaaaag tctttatttg ctatatttac tccatacaca
    10861 aatagcagta tagttatata tgttaaaata ggattcacaa tcgtggataa attagtttta
    10921 gatgcttctc caaaaatctt gagaataaac actggcaaaa tattcataac aattcccaca
    10981 atcataaata taattatata caatttagta tttttattaa aaaattttat atatggcttt
    11041 aattcattca attacaattc ctccttctta gtcatgttaa caaacatatc ttgtagccct
    11101 atgtatccta catcaatatt tgaattttta agtatttttt catcattttc actaaaatca
    11161 ccatagtaaa aataagcaac tgcatttcca aatgcctttt taggttttat attttttata
    11221 cattctaatt tttctaactc ttctttattt ccagtaatat aatgagcttt ttgttttatg
    11281 acatcaattt cttcatctat aataattttt ccctcattta atattattac atgctccagt
    11341 aaatcatcta tttcatcaat caaatgagtt gatattatta ttgtccttgg attttttata
    11401 taactatcta aaattatgtt ataaaactct tgtctattta ctgcatccaa accaattgtt
    11461 ggctcatcaa atatggtgat agctgaatta gaacaaatac ctattatatt tgaaagtata
    11521 gttttcatac ctcttgacag ttgtttatac ttttttttag tatttaaatc aaaatatttg
    11581 cataattctt tttctaagcc tttatcataa cttttataaa aactagaaga aaattcaaat
    11641 atctgaccta ctttaaaatc tggactataa aattcttttt ccctgacaat acaaacttct
    11701 tccaaaactt ttatatcttc ttttgggttc tttcctaata tttctatctc accttcattt
    11761 tgaattaact gatttaccat tatatttaat aatgttgttt ttccagctcc attcttacct
    11821 aataaaccat atatcttatt tttttcaaag tttagagaca tattattaaa aatattttgc
    11881 ttcttaaaac tgtgagataa attttctatc ttaatagaac tactcatata ttaatcctcc
    11941 ttgaaattaa ttataatatc aaccaattct tctctattta tttctaattt gttagcttct
    12001 tgaagtaggt tttttacaaa attatgttta aaattttctt ttctagcttc ttttataata
    12061 gtttttgcac catctgatac aaacatccct atccccctct tcttatataa aatattttta
    12121 tcaaccaata tatttatacc ttttaatact gtagctggat taattttata aagctttgaa
    12181 agctctgttg ttgatggaac ctgttcttct tcttttattc catttgatag tatctcatct
    12241 tcaattgctc gtgctatctg aataaatata ggttcttcat tatttaatac taatttcata
    12301 ggttcctcct ttctccatta gttagttact caactaatta actatataac ttataatatc
    12361 ttgaaaaata ttttctgtca atactaaata aaaaacagag ggaaaattcc ctctgttttt
    12421 tgttctttat atttataatt aaattttgat ttttgtaatc ctatcccata agttaaaaac
    12481 tttaataatc tgttgtatct tcatttttta atattttaca tattgagcta gttcctaacc
    12541 tttctgctcc tgcctctata aatttttctg catcagaaat tgatttaact ccccctgcag
    12601 cttttatttt tacatttttt ccaacatgct ctttcataag ctttatatct tctaaagtag
    12661 ctccacctgt tcccattcca gtagatgttt ttataaaatc tgaacctgac attgttacta
    12721 tttcacacat ttttattttt tcatcttcat ctaaataaca tgtctctata atgactttta
    12781 atactttatc tcctatagct ttttttattt cttttatttc ttttcctata ttatcataat
    12841 ctttattttt tatatctgaa atgtttacaa ccatatcaac ttcatctgct ccattttcaa
    12901 tagcatcttt tgcttcaaat acttttgtag cagtagtttg atatcccaaa gggaacccta
    12961 tcactgtaca tatctttatt ttacctttta gatattctga tgctcttttc acatatgatg
    13021 gtggtatgca aactgatgct acactcatat ctacagcttc atcacataaa attttaatat
    13081 cttcccatgt agttgtagct tttaaaatag tatggtctac tgtttttaat atatgtttca
    13141 taaaatcctc ctaaatttat aattaatata attagattac ttttttaaat gcaattttct
    13201 ctttgcaata ttcagtatca tatcaacctc ttcaacttta aaaatagtca ttagtagtgt
    13261 ataaattcca cctccaacta taactgaaat agctagtgag acaaactcat taaatgttcc
    13321 tactcccaaa attccaaata taaatttata cgtaaagtat gataaaacac ccataattaa
    13381 agatgcaact accgatttca aagttgcttt tattatttta tcttgtccaa aatatccaac
    13441 ttttctcttt aggttcaaga agagtaacaa tatacatgca attgatgatg aactagttgc
    13501 aagagcaaga ccaccatgag ccattggttt tattaaaact aagtccaata ctatatttac
    13561 ccctacagat ataatcccat ttaccatagg tgtttttgtg tcttgtaatg aataaaaaac
    13621 ctttcccaaa atatctctta agccaaatgc agtcatacca actgcataaa aaattaaggc
    13681 agttgctgtc atttgggttg ccctcgcatc aaaagcacct ctctcaaaaa tgattctaac
    13741 aacaggagtt gcaaaaaaaa tagaagcaac tgaaatagga atcatagaaa ttattatcat
    13801 atttatacta gatttaactg aactagtaaa cttcttttga ttattttctg cacttaactt
    13861 agataacata ggatacataa cagatactat tgaagctgta aatgtccctg taacaaaacc
    13921 atttaatcta tctgcataag taagtgctgg aatgcttcct ttaactaatg ttgaggctaa
    13981 agttgtatct accaatgagt tcacttgatt aacagcaaca cctataaata ctggtgaaag
    14041 taatgctagt aatttaataa ggctatcatc tttaaagttt aagtagtata agtatttgta
    14101 gttcgttttt ttaacaaaaa acatgtaaaa gagcagttga actaccatag caaccacagc
    14161 accaattgga agtatgtatg gtccaaatat ggtacttaac attattgaaa taattattac
    14221 tatattatag ggtatacttc caaatccaac tacaataaag ttctctttaa tttgtaaaaa
    14281 tgctgacata acacttgtaa tacctatgaa tattattcct gttctgaacc tcccatgact
    14341 aaagtcacag ggttcctgct tcatagaatt ttgcatacta aaatatgtct tactaattct
    14401 ccacaggcta tccccgtagt tcctacggtt cttacatacg ttatttattt tgaattgtta
    14461 ttagtcttaa tccttctttt aatatatttc tacttgcatt tatatctcta tcgtgagttt
    14521 cattacaact aggacaaatc cattctctta tatttaagtt cttaacttcc tcgtttttat
    14581 acccacattt attgcatatt tgtgaacttg caaagaattt acctaccttt acaatttgtc
    14641 taccatgcca attagccttg tattctaatt gacgaataaa ctctgaccat gatacatcag
    14701 aaattaagcg agatagttta tgatttttaa tcatattctt tacttgtaaa tcttctatgc
    14761 aaataatatc attctctttt attaatttag tagttaattt ttgtagaaaa tcatttcttt
    14821 gattagctat atgttcttga agtcttgcaa cttttaatct tgctttattt ctatttaaac
    14881 taccgattgt ttttctcgat aattctcttt gtaatttagc aagtttgttt aatgacttct
    14941 taaaatactt aggattttct ataaattctc cacaacttga gatacaaaat tctttaattc
    15001 ctaaatctaa acctatctga ttgttagt
//
LOCUS       DAEPIN010000083        15014 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.083, whole genome shotgun sequence.
ACCESSION   DAEPIN010000083 DAEPIN010000000
VERSION     DAEPIN010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15014
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.083"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            112..3015
                     /locus_tag="KR250_003141"
     CDS             112..3015
                     /locus_tag="KR250_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBH0069035.1"
                     /translation="MELRDKLVNIINTENKKDPLTDVQIAKFLSTTRENITNLRKELN
                     ISNSRQRRYPYLKSAISAILQKNKNISVSEITRELMTEGFNISRRVVEELLPKESLEM
                     DVVEESEEESNDPFETLIGNKGSLRNAVEQAKSAILYPPKGLPTLIIGESGVGKSLFS
                     RHMYEFAKQKKIVKESSNFVVFNCADYSDNPQLLLSLLFGYKKGAFTGAEYDTPGLVE
                     EADEGVLFLDEIHRLPPKGQEILFSILDRGKFRRLGETNAERKVSIMLIGATTENVET
                     NLLLTFRRRIPMLITLPPLHDRLLKEKIDLIYNIFQQECNRINAKIFVDKNVIEILAL
                     KKFSGNIGQLQNMIQVLCARAFMKFINSKDEDSESIVVVDINEVLKLKDSFKDAAFQE
                     IEYTEIRKYLKNAIFIPFNLEESSVKGSLLSDEYILPEDIYKTIEKKYYDLKSLDIED
                     IDIENILWTFILNRFADLKFNLDSNEEIFSMSDLNSFVSDSLVKLVKELMNDIVEKNM
                     NLEVNKNMFKYLAIHLEEAIKRIKLNQKIINVNLEKIKIDFSKEYEISKSFAIKIEES
                     IGIKIPDDEIGFITLYIKSALKNEVKKDKVGLIIISHGRIATETVNVVKELLGVKFPV
                     AIDMPLDEKPINIYNKAVELSKIIDQGKGILFLVDIGSLTNIGQIVNKRTNISTKTID
                     RVDLLMALEATRKVSMGEEELDEIFFSLLKDRMGYNYELEKHIDKSNAIVTLCLTGEG
                     TAKYISKTLEEKYDNTKCYQMSALDENLFCKIENLKETNNILAIVGTINPKIPGINFI
                     PYNKELFKNLDIYLSKSKQQDDRPVKSYERMLDEDLVIFEPDIYFKKDLLEYVCSILI
                     NKEYVEKDYLDSVLHREEMLPTYSKGAIGVPHGDSSTVNSTRFVFVKLKNPIDWGVGN
                     VNFIFMPVFQANDKEIVKNVLEILKDAEFMNNANKCFDKDAFRRIIFDKFKHL"
     gene            3048..3494
                     /locus_tag="KR250_003142"
     CDS             3048..3494
                     /locus_tag="KR250_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBH0069036.1"
                     /translation="MNVDLISLGLESNDYESVIEELGSIMCKKEYVKETYINAVLERE
                     RTLPTGLDIGEMCVAIPHTDSKHVNESNVAVGILKNPVKFNSMIDPKDRLDVELVFLL
                     AVKNPDSQVKLLKDLMSVFQNIKLLKNIKNASTKEEVAKLLDFIEI"
     gene            3617..3919
                     /locus_tag="KR250_003143"
     CDS             3617..3919
                     /locus_tag="KR250_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBH0069037.1"
                     /translation="MKQFNVLSVCGSGTVTSSMVAGKLKEVLGNKGISITTTEARPTE
                     ALNLAQSGRFDFITFTSPLQTGDYGIPTINAFACLTGIGEEAFFEEVLTVIEGIDK"
     gene            4100..5506
                     /locus_tag="KR250_003144"
     CDS             4100..5506
                     /locus_tag="KR250_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBH0069038.1"
                     /translation="MLEFMKSIIDTFGSAIIVPIIIFIIAKIFKVTTKKSFLSAVYAG
                     VALQGFTLILNSFTPIITPVINRMVESTGVNLPVFDVGWQATSLVAFSTSAGMIYLGL
                     GILLQTILFLIKWTDVFQPSDLWNNYSYMVWGAMVIGVTGNFPLGIACMVLLNLYSLL
                     ISELVAKRWSSYYRYPNCTIIAMHNVEASVFAVFADPIYNKLGLNKIKLNPKELEKKL
                     GFLGEPITLGLFLGMFIGILGNMTRINTMEAWGEIMKVGISTSAVMAIFPKVASMFAQ
                     AFAPITEAARKIMQKAGNREWYIAVNDAVGYGEPATLISGLILIPIMLVIAMVLPGNK
                     VLPVVDLLAIPYMVQGLVAIHNGNIPKVLVSGIIWFGLGLYVCTSTAPLFTDMATNIG
                     VAIPAGAMLITSFNILGKPLMGLVFFAFLSANPIYIGLSVVIYFVLWALFRKNKTSIL
                     DYLEKQALKNVEEEPVAV"
     gene            5850..6908
                     /locus_tag="KR250_003145"
     CDS             5850..6908
                     /locus_tag="KR250_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBH0069039.1"
                     /translation="MGDKMRASVLYSVGDVRYEMVDIPEITDTQVLVNVKYVGICGSD
                     LPRSMVSGLSGNTKYPLILGHEFSGEVVKIGEKVKHINVGDRVAVAPLVPCGKCDYCN
                     EGNFGLCDDYNIIGTRVNGAFAEYVRVPEEHILKLPDTLDYETAAGIEPATIAYHGIS
                     KSNIRVGDSVVVLGCGPIGQFVIQWAKVFGASKIIAVDIFDEKLELSKLLGANYILNS
                     KEVNVIKEIKKITNGGADVVIETAGSRFTQEQSLFVAKKRGNIVFVGISHTELPLSAD
                     ATECILRGELTLKGSWNSYTSPYPGRAWTATLDFMGKGDIIFKPMISHKIGLNEVGDF
                     LSKMSKREINFNKILVEI"
     gene            7007..7693
                     /gene="araD"
                     /locus_tag="KR250_003146"
     CDS             7007..7693
                     /gene="araD"
                     /locus_tag="KR250_003146"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HBH0069040.1"
                     /translation="MLENLKEEVLKANLELPKKKLITYTWGNVSGIDREKGLVVIKPS
                     GVEYSNMTVEDMVVVDLEGNIVDGKLRPSSDTPTHLVLYKEFEELGGIVHTHSSWATI
                     WAQIGKSIPSCGTTHADYFYGSIPCTRKMTREEICNEYEKETGNVIIEEFMGRNPIHC
                     PGIIVNDHGPFTWGKDANEAVHNAVVLEEVAKMAYYTELMSPDNIMDKVLMNKHFSRK
                     HGKNAYYGQK"
     gene            7711..8358
                     /locus_tag="KR250_003147"
     CDS             7711..8358
                     /locus_tag="KR250_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HBH0069041.1"
                     /translation="MLDSLKGKIVEIAIKAEHYGLCKEKTGNFSIRDKDTGYILITPS
                     GVGREELKVEHICVLDLDGNVIEVEKGIKPSSEVLMHIEIYKTRENINAISHTHSLYA
                     TAFSVAKKPIIPIVYESVNYGGYVDIAPYERPGTLKLAKSVSKLLIDTDACLLERHGL
                     TTVGNDLDEALLKSRYVEEVAEIYYRSLVLNQFKELEVVDVDEFKSWKYPEEIKF"
     gene            complement(8531..9898)
                     /locus_tag="KR250_003148"
     CDS             complement(8531..9898)
                     /locus_tag="KR250_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HBH0069042.1"
                     /translation="MNLIDILNKVDAFIWGPPLLVLLVGTGILLTVKLGVVQITKLPR
                     ALKLIFSAENKGSGDVSSFAALCTALAATVGTGNIVGVATAIKAGGPGALFWMWIAAF
                     FGMATKYSEGVLAIKYRTKDKNGQVSGGPMYYIVNGMGEKWRPLAIFFAISGILVALL
                     GIGTFTQVNSITDAINNSFGIDPRITGVVLAVFVALVVFGGLKSISNVATKIVPFMAV
                     IYVVICGIILISFWNKIPETFMLIIKSAFTPTAATGGFLGATMSLAIRNGIARGVFSN
                     ESGLGSAPIAAAAAKTEWPAEQGLISMTGTFIDTIIICTLTGFSLVISGVWCSDLNGA
                     VMTQAAFNGAIPTFGPILLTVSLTLFAFTTILGWSYYGERCFEFLFGVKGMNGYRTVF
                     VAMVLLGAFLKLEVVWIIADIVNGLMAIPNLIALLALSPIIVSETKKYFEHINSPENQ
                     IKKNA"
     regulatory      complement(10088..10176)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            10604..12724
                     /locus_tag="KR250_003149"
     CDS             10604..12724
                     /locus_tag="KR250_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBH0069043.1"
                     /translation="MGKILVLAEKPSVGRDIAKVLGSKNEKNGYIEGPKYVITWALGH
                     LVTLADPESYGERYKSWSLEDLPILPKHLKTVVIKKSGKQFNTVKSQMNRNDIDEIVI
                     ATDAGREGELVARWIIEKSQVKKTIKRLWISSSTDKAIKDGFAKLRSGKEYENLYYSA
                     IARAEADWIIGINATRALTTKYNAQLSCGRVQTPTLAMLLKREEEIRNFKPKEYYGLE
                     LIATKGNSDIKFIWNDKNNNSSTFSKEKIESTLKKVKGVDIKISEVNKSYKKKYSPAL
                     YDLTELQRDANKMFGFSAKETLSIMQKLYEHHKVLTYPRTDSRYLTSDIVGTLKDRIK
                     AVNISDYSKTCNKLLKSNIIANKSFVDNSKVTDHHAIIPTEQKVFMSDFSDKERKIYN
                     LVVKRFLAVLSPPFEYEQTTISATVEGEIFKAKGNKVINLGWKESYKDIEDEDEYESI
                     PNILEKDVLKVKGLKITSGKTNPPPYLNEGTLLTAMEKNGLGTVATRADIIEKLFNSF
                     LMEKKNKDIYITSKGRQLLDLVPVDLKTPELTASWEKKLLDISKGKLSKNTFINDMKD
                     YSKDIVLEVKNSDNKFKHDNLTKNRCPECGKFMLEVNGKKGKMLICEDRECNTRKTIS
                     QVTNSRCPNCHKKLELRGEGEGRIFTCSCGYREKLSSFNKRKQEEKQKASKKDVNQYL
                     KNQNKNQETFNNPFAEALAKLKNK"
     gene            12828..14081
                     /locus_tag="KR250_003150"
     CDS             12828..14081
                     /locus_tag="KR250_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliS"
                     /protein_id="HBH0069044.1"
                     /translation="MDLSGIFKYYCKECENTWNNSSVELFENIETYSKDSQKKREKEL
                     DKLLNTISVHLERYPSDAVLRKMWVKKGEVFLQKTLEKENIFKLEKMDVEDRKKFLDI
                     TKQFIRDARKFDDDLPIGDIMQAMRNVWISNALQLLFGKEVYYSKANFAYSMLYPYTD
                     NYLDNTNIDKNDKILFNNWLEKRLLGEHIKSKDYHESKVSKMIDYIESVYPREKFTEV
                     YESLLLIFKSQVNSLKQHGKENHLCKEDLLSISIEKGGSSVLVDGYLISGLMTKEEIE
                     FCIGYGFLLQISDDLQDIKEDLKYNHKTIITEMSKEGTLDKVVNKLINFTIELIDSFK
                     INNKNKSVITMIKNDCLMLILFSVVYNAEFFSVGYIKEVEKFIPYTIDYSLEIEEKIK
                     EKFKNIDVLNNENEYKEMIDIICAE"
     gene            complement(14234..>15014)
                     /gene="ytvI"
                     /locus_tag="KR250_003151"
     CDS             complement(14234..>15014)
                     /gene="ytvI"
                     /locus_tag="KR250_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sporulation integral membrane protein YtvI"
                     /protein_id="HBH0069045.1"
                     /translation="VFFRLPEIYKSSIQPTLNTLFSKIDASTPNVNLALILGWDNISQ
                     SMMSLVASISTNALNAIASIASKTPAFMLKLIITLIASFFFTFDYQKIVNFILKQFPE
                     KSQFMIINIKNSSINALLKLLKAYAILLSVTFIELLIGLTILKVENAFTISVIIALVD
                     ILPVLGTGSILTPWMIISLINGNINLAIGLLILYIIITVVRQILEPKVVGHQIGLYPL
                     ITLMCMFVGAQLFGIAGLFGFPIAATIIKNLHDNGIITAFK"
BASE COUNT         5945 a         1711 c         2627 g         4731 t
ORIGIN      
        1 tataaatact tatgacatct agttacttac agggtttaag tagaaaaaaa tatcaaagta
       61 agtatggcat gatagttgct tatatattta tcaagaaagg agttattaag catggagttg
      121 agagataaac ttgtaaacat cataaataca gaaaataaaa aagatccatt aacagatgtt
      181 caaatagcaa agtttttaag tacaactaga gagaacataa ctaatttgag aaaagaactt
      241 aatataagta attcaaggca aagaagatat ccttatttaa aatcagcaat ttcggcaata
      301 ttacaaaaga ataagaatat ttctgtatca gaaattacaa gggaattgat gacagaaggc
      361 tttaatatat ctagaagagt tgtggaggaa cttttaccta aagaaagttt agaaatggat
      421 gtagtagagg aatctgaaga agaatcgaat gacccatttg aaactctgat aggtaataaa
      481 gggagtctta gaaatgctgt agagcaggct aaatctgcta tattgtatcc accaaaaggg
      541 ttaccaacgt tgattattgg ggaaagtgga gttggtaaga gtttattttc tagacatatg
      601 tatgagtttg caaagcaaaa aaagatagta aaagaaagct ccaattttgt tgtatttaac
      661 tgtgctgatt atagtgacaa tccacaatta ttattatctt tgttatttgg atataaaaaa
      721 ggtgcattta caggtgctga atatgataca ccaggtcttg tagaagaagc tgatgaagga
      781 gttttatttt tggatgaaat acatagattg cctccaaaag gccaagaaat actattttct
      841 attttagata ggggaaagtt taggagatta ggtgaaacta atgctgaaag aaaagtaagt
      901 attatgttga ttggggcaac tactgaaaat gtagagacta atttattatt aacattcaga
      961 agaaggatac caatgctaat aactttgcca ccattacatg atagattact aaaagagaaa
     1021 atagacctta tatacaatat ctttcaacaa gagtgtaata gaataaatgc aaaaatattt
     1081 gtagataaaa atgtgattga aatacttgca ctaaaaaagt ttagtggaaa tataggacaa
     1141 ttacaaaata tgattcaggt tctttgtgca agagctttta tgaagtttat aaattccaaa
     1201 gatgaagata gtgaatcgat agtagtagta gatataaatg aagttttaaa gcttaaagat
     1261 tcttttaaag atgctgcctt tcaagagatt gaatatacag agataagaaa atatttgaaa
     1321 aatgctatat ttataccatt taatttagaa gaatcatcag taaaaggaag tcttttaagt
     1381 gatgaatata tattaccaga ggacatatac aaaacaattg aaaagaaata ttatgaccta
     1441 aaatccctag acattgaaga tatagatata gagaatattt tatggacttt tattttaaat
     1501 agatttgctg acttaaagtt taatctcgat agtaatgaag aaattttttc aatgagtgat
     1561 ttaaatagtt ttgtaagtga tagtttagtt aaactggtaa aagaacttat gaatgatata
     1621 gttgaaaaaa atatgaattt agaagtaaat aaaaatatgt tcaaatatct agcaatacac
     1681 ttagaagaag ctataaaaag aattaagcta aatcaaaaga taataaatgt aaatttagaa
     1741 aaaataaaga ttgacttttc aaaagaatat gaaattagta aaagttttgc aataaaaatt
     1801 gaggaatcaa ttggtattaa gattccagat gatgaaattg gatttataac actctacata
     1861 aagtctgctc taaaaaatga agtgaaaaaa gataaagtag ggttaataat aatttctcat
     1921 ggacgtatag ctacagagac tgtaaatgta gttaaagaac ttttaggtgt aaagtttcct
     1981 gtggctatag atatgccttt agatgaaaaa ccaataaata tatacaataa ggcagtagaa
     2041 ctgtcaaaaa taatagacca agggaaaggc atattgtttt tggtggatat tggttcactt
     2101 acaaacatag gtcaaatagt aaataagaga accaatataa gcactaaaac tattgataga
     2161 gtagacctcc taatggcact agaagcaact agaaaagttt ctatgggaga ggaagaactt
     2221 gatgaaatat ttttttcttt gctaaaagat aggatgggat ataactatga attagaaaaa
     2281 cacatagata aatcaaatgc aattgtaaca ttatgtctta ctggagaagg aactgctaag
     2341 tatataagta aaacgttgga agaaaagtat gataacacta aatgttatca aatgagtgca
     2401 ttggatgaaa atttattttg taaaattgaa aatctaaaag agacaaacaa tatattagct
     2461 attgttggga caataaaccc taagatacca ggtataaatt ttatacccta taataaagaa
     2521 cttttcaaaa atttggatat ctatctatca aaatcaaaac aacaagatga cagaccagtt
     2581 aaatcgtatg aaagaatgct tgatgaggat ttagtgattt ttgaaccaga tatatatttt
     2641 aaaaaggatt tattagaata tgtatgttca attcttataa ataaagaata tgtagaaaaa
     2701 gattacttag attctgtttt acatagagaa gaaatgcttc caacatattc taaaggagct
     2761 ataggtgtgc ctcatggaga ttcttctact gtaaatagta caaggtttgt ttttgttaaa
     2821 ctaaagaatc ctattgattg gggtgtaggt aatgtaaatt tcatatttat gccagttttt
     2881 caggctaatg ataaagagat tgttaaaaat gttcttgaga tactaaaaga tgcagaattt
     2941 atgaacaatg ccaataagtg ttttgataaa gatgctttta gaaggattat atttgataaa
     3001 tttaaacatc tgtagccata gcaaaaaaat tgagaggagt tagagtaatg aatgtagatt
     3061 taatatcatt gggtttagaa tctaatgatt atgaaagtgt tattgaagaa ttaggaagta
     3121 taatgtgtaa aaaagaatat gtaaaagaaa catacataaa tgctgtcttg gaaagagaaa
     3181 ggacattacc aacaggtcta gatattggag aaatgtgtgt tgctattcca catacagatt
     3241 caaagcatgt aaatgagtct aatgtagctg tagggatatt gaaaaatcct gtaaagttta
     3301 attcaatgat agacccaaaa gatagacttg atgtagaact tgtattttta ttagcagtaa
     3361 agaacccaga ttcacaagta aaactgttaa aggatttaat gtcagttttt caaaatataa
     3421 agttattaaa aaatataaaa aacgcatcaa caaaagaaga agtagctaaa ttattggatt
     3481 ttatagaaat ttaatgtagg agaattttat aaataaaaaa ttaaaattta aacaatcaaa
     3541 gtaagtatta tttttttaat aggtttaatc ttatataaaa taaaaattta gagtgaaaaa
     3601 ttaggaggaa attattatga aacaatttaa tgtattatca gtttgtggtt cagggacagt
     3661 tacatcatct atggtagcag gtaaattaaa agaagtacta ggtaataagg ggatttctat
     3721 tactacaaca gaagctagac caacagaagc actaaattta gcacaatcag gaagatttga
     3781 ctttataaca tttacgagtc cacttcaaac aggcgattat ggaataccaa ctataaatgc
     3841 atttgcttgc ctaactggta taggggaaga ggcattcttt gaagaagttt tgactgttat
     3901 tgaagggata gataagtaag ttttggagac gtgaatttat agtatttaaa tcaatgattt
     3961 agatactata ataagctaat attaactaaa tatttgtgga aaaaacattt tatatgttgt
     4021 cataaattaa aaaattataa aaatagtcac ttaagtatta aattaagctt ataaatattt
     4081 ataagtagga gggaaaaaga tgttagaatt tatgaaatct ataatcgata catttggttc
     4141 tgcaattata gttccaatta taatattcat aatagcaaag atatttaaag taactactaa
     4201 gaaatcgttt ctttctgctg tgtatgcagg agttgcttta caaggtttta cactgatttt
     4261 aaattcattt acaccaataa taacaccagt tatcaatagg atggttgaga gtacaggagt
     4321 aaacctacca gtttttgatg ttggttggca agcaacatca ttggttgcat tttcaacaag
     4381 tgcaggtatg atatatttag gtcttggtat attattacaa acaattttat ttttgataaa
     4441 atggacagac gtgtttcaac catcagattt atggaataac tattcttata tggtatgggg
     4501 agcaatggtt ataggagtaa caggaaattt tcctttaggt atagcatgta tggttttatt
     4561 aaacctatat agtttattaa tatcagagtt agttgcaaag agatggtcta gttactatag
     4621 atatcctaat tgtacaataa tagctatgca taatgttgaa gcttctgtgt ttgcagtatt
     4681 tgcagaccct atatacaata aattgggatt aaataaaata aaattaaatc caaaagaatt
     4741 agagaagaaa ttagggtttt taggtgaacc aattacttta ggtctttttt taggtatgtt
     4801 tataggtata ttaggtaata tgacgagaat aaatactatg gaagcttggg gagaaataat
     4861 gaaagtaggt atatctacaa gtgcagtgat ggcaattttc cctaaagtag caagtatgtt
     4921 tgctcaagca tttgcaccta taacagaagc agctagaaag attatgcaaa aagctggaaa
     4981 ccgtgagtgg tacatagcag taaatgatgc tgttgggtat ggtgaacctg caactttaat
     5041 atcaggtctt atattaattc caataatgct tgtaatagct atggtacttc ctggtaataa
     5101 ggtattacca gttgtggatt tattggcaat tccatatatg gttcaaggtt tagttgccat
     5161 acataatggt aatataccaa aagtattggt ttcaggtata atttggtttg gattaggatt
     5221 atatgtttgt acatctacag caccattatt tacagatatg gctacaaata taggtgtagc
     5281 tatacctgca ggagcaatgt taataacaag ctttaatata ctaggaaaac ctttgatggg
     5341 attagttttc tttgcatttc taagtgcaaa tccaatttat ataggtcttt cagttgtaat
     5401 ttactttgtt ctatgggcat tgtttagaaa gaacaaaaca agtattcttg attatttaga
     5461 aaaacaagca cttaaaaatg tagaagaaga gcctgtagca gtttaagttt ataaaaatga
     5521 aataagaata attaaaggag tttataattg attttatata agtatcagat aatacctgag
     5581 aaatatttaa atgaaatata gacttctttt tatttgataa tatttgtaat tataatgcat
     5641 tgtatcttta tagttgagtt ttctgtataa cctgctattt aaaacatact cttttatatt
     5701 tatacagtaa tgtggtttaa ttttataaaa tgtaatctct actggtaaaa gactttgtat
     5761 ataaggcatt tgcctgaata aaccatttat cttttgtatg gcatagatat tgctatatag
     5821 aagtatatag catgaaagga gatatttata tgggagataa aatgagagca tcagtattgt
     5881 atagtgtagg tgatgtcaga tatgaaatgg tagatatacc agaaataaca gatacgcaag
     5941 tgcttgtaaa tgtaaaatat gttgggatat gtggttcaga tttacctagg tctatggtaa
     6001 gtggtttgag tggaaataca aaatatccat tgattttagg acatgaattt tctggcgaag
     6061 ttgttaaaat tggagaaaaa gtaaagcata ttaatgtagg agatagagta gctgttgcac
     6121 cattagtacc ttgtggaaaa tgtgattatt gtaatgaagg taattttggc ttgtgtgatg
     6181 attacaatat aattggaaca agagtaaatg gagcgtttgc agaatatgta agagttccag
     6241 aagaacatat tttaaaacta ccagatacat tggattatga aactgctgcc ggaattgaac
     6301 cagcaactat agcttatcat gggatttcaa agtctaatat tagagtagga gatagtgtag
     6361 ttgtgttagg atgtggtcca ataggtcagt ttgttataca atgggcaaag gtgtttggtg
     6421 catctaagat tatagcagtt gatatttttg atgaaaaatt agaactttct aaacttctag
     6481 gagcaaacta tatacttaac tcaaaagaag taaatgtaat caaagaaata aaaaaaataa
     6541 caaatggtgg tgctgatgtt gtaatagaga ctgctggtag ccgatttaca caagaacaat
     6601 ctctatttgt agcaaaaaag aggggaaata tagtgtttgt aggtatatca cacacagaat
     6661 taccacttag tgcagacgct actgagtgta tattacgtgg agaacttacc ttgaaaggtt
     6721 cttggaattc atatacaagc ccttatcctg gaagagcatg gactgcaaca ttggatttta
     6781 tgggaaaagg tgacattatt tttaaaccca tgatttcaca taaaataggt cttaatgaag
     6841 tgggtgactt tttaagtaaa atgagcaaga gagaaattaa tttcaataaa atcctagtag
     6901 aaatataaaa atttaataaa aaattctaaa agcaattaaa ttttataact atgacattaa
     6961 ataaaataaa aataattttc ttaaataaaa tataggagga agaaaagtgt tagaaaattt
     7021 aaaagaagaa gtattaaaag caaacttaga attacctaag aaaaagctaa ttacttatac
     7081 ttggggaaat gtaagtggaa tagatagaga aaaaggatta gtggtgataa agccaagtgg
     7141 tgtagagtat agtaatatga ctgtagaaga tatggttgta gttgatttag agggaaatat
     7201 agttgatggc aaattaagac cttcttcaga taccccgact catctagttt tatataaaga
     7261 gtttgaagaa ttggggggaa tagtacatac acattcaagt tgggcaacaa tttgggctca
     7321 aataggaaaa tctataccat cttgtggtac tactcatgca gattattttt atggaagtat
     7381 accatgtacc agaaaaatga ctagagaaga aatttgcaat gaatacgaaa aggaaactgg
     7441 aaatgtaatc attgaagaat ttatgggaag aaatccaatt cattgtccag gaataattgt
     7501 aaatgaccac ggacctttta catggggaaa agatgcaaat gaggctgtac acaatgcagt
     7561 agttttagaa gaagtcgcaa agatggctta ttatacagag ttaatgagtc ctgataatat
     7621 tatggataag gttcttatga ataagcattt ttcaagaaaa catggaaaaa atgcatacta
     7681 tggtcaaaaa tgattatctg gaggtgaatt atgctagact ctttaaaagg taaaattgtg
     7741 gaaatagcaa ttaaagcaga gcattatggt ttatgtaaag aaaagactgg taattttagt
     7801 attagagata aggatactgg atatatactt ataaccccat caggtgtagg aagagaagaa
     7861 ttaaaagttg aacatatttg tgtattggat ttggatggaa atgtaataga agttgagaaa
     7921 ggtattaagc caagtagtga ggtattgatg catattgaaa tatacaaaac aagagaaaat
     7981 ataaatgcca tatcacatac tcattcttta tatgcaacag cattttcagt agcaaagaaa
     8041 ccaattatac caattgtata tgaatcagtt aattatggcg gttatgtaga tattgctcca
     8101 tatgaaagac caggaacttt aaaattagct aagagtgttt caaaattact gatagataca
     8161 gatgcatgct tattagaaag acatgggttg acaactgtag gtaatgattt agacgaagca
     8221 ttattaaaat caagatatgt ggaagaggta gcagaaatat actataggtc tttagtatta
     8281 aatcaattta aagaacttga agtagtagat gttgatgagt ttaaatcttg gaaataccca
     8341 gaagaaataa agttttaatc taaataattt tcattgaagt ggttaattaa aatatatgta
     8401 ttatttatat caattactat gttttattat gttaattacc gtattttgca aatatattta
     8461 ttacattttt tcaaaaaaat atagaatccg aaagccatat aaaattgact tgggattcta
     8521 tactttttat ttatgcattt ttctttatct gattttctgg agaatttata tgttcaaaat
     8581 attttttagt ttcagatact attataggtg ataatgcaag taaagctata aggttaggta
     8641 ttgccataag cccatttaca atatcagcaa ttatccaaac aacttctaac tttaagaaag
     8701 cacctaataa caccatagcc acaaatacag ttctataccc attcatgcct tttacaccaa
     8761 acagaaattc aaaacatctt tctccataat aactccatcc caaaattgta gtaaatgcaa
     8821 ataatgttaa actaactgta agtaatattg gtccaaatgt aggaattgct ccgttaaaag
     8881 ctgcttgtgt cataacagct ccatttaggt ctgaacacca cactcctgaa ataacaagag
     8941 agaatccagt aagagtacag attataatag tatctataaa agtacctgtc atagatataa
     9001 gaccttgttc cgctggccat tcagttttag cagcagcagc agctattgga gcactaccta
     9061 gaccagactc atttgaaaat acaccacgag cgataccatt tctaattgcc agtgacatag
     9121 ttgctcctaa aaaaccacca gtagcagcag taggtgtaaa tgcactttta attattaaca
     9181 taaaagtttc agggatttta ttccaaaaag atattaaaat gattccacag attacaacat
     9241 atataactgc cataaaaggt actattttag tagctacatt agatatactt ttaagtcctc
     9301 caaatacaac caaagctaca aatactgcta atacaacccc tgtaattcta gggtctatcc
     9361 cgaaactatt atttattgca tctgttattg aatttacttg agtaaaagta ccgattccta
     9421 aaagtgcaac tagtatacca cttatagcga aaaatattgc aagaggtctc catttttctc
     9481 ccataccatt tactatgtaa tacattggcc caccagacac ttggccattt ttatctttag
     9541 ttctgtattt tattgcaaga acaccttctg aatacttagt agccattcca aagaatgctg
     9601 ctatccacat ccaaaaaagt gcaccaggtc caccagcttt gatagctgta gcaacaccaa
     9661 caatattacc agtaccaact gtagcagcta gagcagtaca taaggctgca aaacttgaaa
     9721 catcaccact acctttgttt tcagcagaga atattaactt aagagctctt ggcagtttag
     9781 ttatttgaac aactccaagc ttaacagtaa gtagtatacc agtaccaact aatagtacca
     9841 gtaggggtgg tccccaaata aaagcatcaa ctttgtttaa aatatcaatt agattcataa
     9901 aaacactccc tcattttttt tgctttacat aatcaataga atttttaatg aagtaattga
     9961 caggaaataa gtataattta ttaataattt ctagtgtaat aaaaaaagct aaatctaaaa
    10021 aagattcagc tctggatgta cactaattag caaccaaaat aaaagtatta gtaaatactg
    10081 tttttggtag ataatccctg tccttttgcc tgagagattg agcataaaag ccttgcccct
    10141 tcggcgccca taatgggtct ctccagagtt ccgtaaattt tacagtccta cttttaaaag
    10201 tacctgagag tgttactcct tcggtgtgct atatagcaac tcttctataa aatcttaacc
    10261 ggatattgat ttgtcatagc ttattgtatt aataatatta atataagttt ttatgttttg
    10321 tcaactaaaa aattaaaaaa caaagaaaaa atataaaaat gtaagcctat ttacgaatta
    10381 taaacttaga aagatgagta atatacgtga aaatgaattt tgtaatacac atacaaatga
    10441 agtgtaaaat aagttctaaa taaaaaaata ataaaatctt aaataattaa ttagatatga
    10501 ttaaattatg gtaatattat agttacgata ttaagaaaag ttctatttag ttagatttta
    10561 agcgtaatat gagattatgt atagagaaag gatgtatttt tagatgggaa aaatattagt
    10621 gctagcagaa aaaccttctg ttggaagaga tatagcaaaa gttttagggt caaaaaatga
    10681 aaaaaatggt tatatagagg ggccaaagta tgtgataact tgggcactag gtcatttggt
    10741 tacacttgct gaccccgaaa gttatggaga aagatataaa tcatggagct tagaagacct
    10801 gccaatactt cccaagcacc ttaagactgt tgtaataaaa aagagtggaa agcagtttaa
    10861 tacagttaaa tcgcaaatga atagaaatga tatagatgaa atagttatag caacagatgc
    10921 aggtcgtgaa ggagaacttg tagcaaggtg gataatagaa aaatcacagg taaaaaaaac
    10981 tataaaacgt ctttggattt cttcaagtac agacaaagca ataaaagacg gatttgcaaa
    11041 attaagaagt ggaaaagaat atgaaaattt gtattactca gcaatagcac gagcagaagc
    11101 tgattggatt attggaataa atgcaaccag agctttgaca acaaagtaca atgcacagct
    11161 ttcttgtgga agagtacaaa ctcctactct tgcaatgttg cttaaaagag aagaagaaat
    11221 aagaaatttc aaacctaaag agtattatgg attagaatta attgctacaa agggaaattc
    11281 agatataaaa tttatttgga atgataaaaa caataattct tcaactttta gtaaagaaaa
    11341 gatagaatcc acattaaaaa aagtaaaagg tgtagacata aaaatatctg aagtaaacaa
    11401 gtcatataag aaaaaatatt cacctgcact ctatgattta acagaattac aaagagatgc
    11461 aaataaaatg tttggatttt cagcaaaaga aactttatct ataatgcaaa aactgtatga
    11521 acatcataaa gtccttactt atcctagaac tgattctaga tatttaacat cagatattgt
    11581 tggaacatta aaagatagaa taaaagctgt aaatattagt gattattcaa agacttgtaa
    11641 taaattatta aaaagcaata ttatagctaa taaatcgttt gttgacaatt caaaggtaac
    11701 agaccatcat gcaataatac caacagaaca gaaggtattc atgagtgatt ttagtgacaa
    11761 agagcgaaaa atatataatc ttgtagtaaa aagattttta gcagtactat ctcctccttt
    11821 tgaatatgag cagacaacta taagtgcaac tgtagaagga gaaatattta aggctaaggg
    11881 aaacaaagta ataaatttag gttggaaaga aagttataaa gatattgaag atgaagatga
    11941 gtatgaaagt ataccaaata ttttagaaaa agatgtactt aaggtcaagg gattaaagat
    12001 aacttcagga aaaacaaatc caccaccata cttaaatgag ggaacattat tgactgctat
    12061 ggaaaagaat ggtctaggga cagttgctac aagagcagat ataatagaaa aattattcaa
    12121 ttcattttta atggaaaaga aaaataaaga tatatatata acatcaaaag gaagacaatt
    12181 attagattta gttccagtag acttaaagac accagaactt acagcttcat gggaaaagaa
    12241 attattggat atttcaaagg gaaaacttag taaaaataca ttcataaatg acatgaaaga
    12301 ttattcaaaa gatattgtac tagaagtaaa aaatagtgat aataaattta aacatgataa
    12361 cttaactaaa aatagatgtc cagaatgtgg taaatttatg ttagaggtaa atggtaaaaa
    12421 aggtaagatg ttgatttgtg aagatagaga gtgtaatact agaaaaacta tatcacaagt
    12481 aaccaattca agatgtccta actgccataa aaaattagaa cttcgtggag aaggtgaggg
    12541 gagaatcttc acttgtagtt gcggatacag agaaaagttg tcgtctttca acaagagaaa
    12601 gcaagaagaa aaacagaagg catcaaaaaa agatgttaat caatatttaa aaaatcaaaa
    12661 taaaaatcaa gagacattta ataatccatt tgcagaagca ttagctaagc taaaaaacaa
    12721 ataattttaa agttattatt atacacaatt ataactttaa ctgtatatta gttatatgta
    12781 ctgatataat atagtgtata gtttaggcat gaaagtaggg ctgttaaatg gatttaagtg
    12841 gcatatttaa atactattgt aaagagtgtg aaaatacatg gaataattcg agtgttgaat
    12901 tatttgagaa tatagaaacg tatagtaaag attcacaaaa aaagagggaa aaagaattag
    12961 ataaattgct aaatacaata tcagttcatt tagagaggta tccaagtgat gctgtattga
    13021 gaaaaatgtg ggtaaaaaag ggcgaggttt tcttacaaaa gacattggaa aaagaaaata
    13081 tttttaagtt agaaaaaatg gatgtagagg atagaaaaaa atttttagat ataacaaaac
    13141 agtttattag agatgctaga aaatttgatg atgatttacc tataggtgat attatgcaag
    13201 ctatgaggaa tgtatggatt tcaaatgcat tacaattatt atttggtaaa gaagtatatt
    13261 attcaaaagc taactttgca tatagtatgt tatatccata tactgataat tatttagaca
    13321 atacaaatat agataaaaat gataagattt tatttaataa ctggttagaa aaaaggctcc
    13381 tgggagaaca cattaaatct aaggattatc atgaaagtaa agtatctaaa atgatagatt
    13441 atattgaaag tgtataccct agagaaaagt ttacagaagt ttatgaatcg ttattattaa
    13501 tatttaaaag tcaagtaaat agtttaaaac aacatggtaa ggaaaatcat ttgtgtaaag
    13561 aagatttatt atccatttct attgaaaaag gaggttcatc cgttttagta gatggatatt
    13621 taataagtgg attgatgaca aaggaagaaa tagagttttg tataggatat ggatttttat
    13681 tacaaatatc tgatgattta caagatataa aagaagattt aaaatacaac cataaaacta
    13741 ttattacaga gatgtcaaaa gagggtactt tagataaagt tgtaaataaa ctaataaatt
    13801 ttactattga gttaatagat agttttaaaa ttaataataa aaataaatct gtaataacta
    13861 tgataaagaa tgattgctta atgttaattt tattttctgt agtttataat gctgaatttt
    13921 tttctgtagg atatataaaa gaagtagaga aatttattcc atatacaata gattattcat
    13981 tagagattga agaaaaaata aaagaaaaat ttaaaaatat agatgtttta aataatgaaa
    14041 atgaatataa agaaatgatt gatattattt gtgcagagta gaaaaaaagg gagtgtctta
    14101 gaatagactt tttagtttat gaggcactct ttttgtataa aatcaaatgt attttcatca
    14161 ttttaataat taaaaagagc ttatccctat ttgagaaagc cctttttaat ttaataaaaa
    14221 tgtcaaggtt aaactattta aatgcagtaa taatcccatt atcatgtaag ttttttatga
    14281 ttgtagctgc gatagggaat ccaaataatc cagctattcc aaatagttga gctccaacaa
    14341 acatacacat taatgttatt aaaggatata ggccaatttg gtgtcctact acctttggtt
    14401 ctaatatttg tctaacaacg gttataatta tgtatagtat taaaagtcct attgctaaat
    14461 taatatttcc attaatgagt gagataatca tccaaggtgt caaaatactt cctgtaccca
    14521 agactggcaa aatatcaaca agtgctatga tgacagatat agtaaatgca ttttcaacct
    14581 ttaggatagt aagtccaata agtagttcta tgaatgtcac tgatagtagt attgcatagg
    14641 cttttagtag ttttaaaaga gcattaatac tgctattttt tatatttatt atcataaatt
    14701 gacttttttc tggaaattgt tttaagataa agtttactat tttttgatag tcaaaagtaa
    14761 agaaaaaaga agcaattaaa gtaataataa gttttaacat aaatgcaggt gttttacttg
    14821 caatactagc aatagcattg agtgcatttg ttgaaataga agctactaaa gacatcatag
    14881 attgacttat attatcccaa ccaagtatta atgctaggtt aacattaggt gtactagcat
    14941 ctatctttga aaataaagta tttagcgttg gttgtatact tgatttatat atttcaggaa
    15001 gtctaaaaaa taca
//
LOCUS       DAEPIN010000084        14746 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.084, whole genome shotgun sequence.
ACCESSION   DAEPIN010000084 DAEPIN010000000
VERSION     DAEPIN010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14746
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.084"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            83..844
                     /locus_tag="KR250_003152"
     CDS             83..844
                     /locus_tag="KR250_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896901.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HBH0069046.1"
                     /translation="MIGIYFSGTGNSRYCVEKFLQEYDITANSYSIEDNELLQHINNH
                     ENIIFSYPVQYSNVPKILKDFIINHSTLWNGKRVFIIATMALFSGDGSGVLSRILKKY
                     GAITVGGLHVKMPDSIGDEKVLKHSLEYNRKLVIKAEKKVTKAAKKLKHGSPPKEGLG
                     FLSHIVGLFGQRLYFINKTNMYTDKLKINTEECIVCGKCVNLCPMKNLVIKNCMIVAN
                     GQCTMCYRCVNNCAKKAITLLGKKVVEQSNIKKYM"
     gene            909..1691
                     /locus_tag="KR250_003153"
     CDS             909..1691
                     /locus_tag="KR250_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HBH0069047.1"
                     /translation="MEYYIVDSFATKLFKGNPAGVCVLDRRIPLELMQKIAEENNLPE
                     TAFVVKGKGNYELRWFTPKAEIDLCGHATLAAAYVISNFIDVNVKRIDFFTQSGKLEV
                     TRNGNLYEMIFPEIMPIEIELSPQQANLIGCVPSAVYSSRDLILLLNSEQEVINYKPN
                     YAQLRKLTDWLGIIITAQGSNTDFVSRYFCPELDSEDPVTGSSHCNLIPYWSEKLGKH
                     KMVAAQLSNRGGIIQCEVLKDNTVKISGEAVLFMQGTIKIDI"
     gene            1958..2377
                     /locus_tag="KR250_003154"
     CDS             1958..2377
                     /locus_tag="KR250_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjdF family protein"
                     /protein_id="HBH0069048.1"
                     /translation="MDKISGKLTVLFEEPFWVGIFERQDGKKYEACRVVFGAEPKEVE
                     VYEFILERFFSLDFGSIKLEKNVTKDNIGYKRMQRKVKKEQEKETIGTKAQNALKLQY
                     EERKQDRKNLAKNRKEEEKERLFNLKQEKRKAKHKGH"
     gene            complement(2480..2746)
                     /locus_tag="KR250_003155"
     CDS             complement(2480..2746)
                     /locus_tag="KR250_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HBH0069049.1"
                     /translation="MKNFILIYFIVINSIAFFSMYIDKKRAIRNEWRIKEATLMSIAV
                     IGGSIGSIIGMYSFRHKTKHIKFTFGIPFILFLQFLLVYFYILK"
     gene            2973..3674
                     /locus_tag="KR250_003156"
     CDS             2973..3674
                     /locus_tag="KR250_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069050.1"
                     /translation="MKFIVEKEVFDKLENVCFGVVVAKGIDNTKEIERISNLLDISID
                     RVEDYFKDKKVKESEEIIPYREAFRSLGMNPNKFMSSIEAMTTRVAKNKKLPHINPIV
                     DLGNSISLKYLLPMGAHDMDFRNDDVYVRFSKKGDKFVPFGETDVELMEEGELIYSVG
                     DMVKTRRWIWRQGEEGKITNSSKNIFFPIDGFTDANLDKVMSAREELAKLLKEIFNCE
                     IKVGFVDKDNPEMEI"
     gene            3756..4364
                     /locus_tag="KR250_003157"
     CDS             3756..4364
                     /locus_tag="KR250_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBH0069051.1"
                     /translation="MKKTIIKNVNPMIENKFIGLFEIEYKNKLDEDKVWMVASRKSSE
                     QLKNIYLKNEEDSVDAVVIVGLHKSSKKLILIRQFRVPINGYIYELPAGLVDEGESID
                     ISVERELREETGLTLLEIKKNKSIDKVYLSPGMSDESVAFVYCICDGDITDEFLEPDE
                     EIEALLISQEDAKEILQSNHKIDTKAFLILQMFVSLGAKLFE"
     gene            4688..5152
                     /locus_tag="KR250_003158"
     CDS             4688..5152
                     /locus_tag="KR250_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4624 domain-containing lipoprotein"
                     /protein_id="HBH0069052.1"
                     /translation="MKKLVIILISIISMVSLTACKKSNESKYNNLNNKESGQTTIEMD
                     LDKNYDTSDPFVNARLFCVSNDIDVLDTEISFKMDGDSGIVEIKDNKTDETLWSNTWH
                     GSVDNDTFTISLSNIQKEKEYAICFTGIKINHAVVKVTFESNLVKEKEKPSK"
     gene            5789..6805
                     /gene="gap"
                     /locus_tag="KR250_003159"
     CDS             5789..6805
                     /gene="gap"
                     /locus_tag="KR250_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBH0069053.1"
                     /translation="MKIKVGMNGFGRIGRAVLRIAQEELGDNIEIVAINARATTESLA
                     HLFTYDSCYGTFRGEVEAKDEDTLVVNSKEIKILRYNDPEELPWKELGVDIVIESTGL
                     FTQREKAEKHIKAGAKKVIITAPGKNEDITIVIGVNEKEYDNEKHNIISNASCTTNCL
                     APFAKVLDEKFGIVKGLMTTVHSYTNDQRILDKSHKDLRRARAAAESIIPTTTGAAKA
                     VSRVLPQLEGKLNGFSLRVPTPTVSLVDLVCELKENVTSEQVNSVLKDAAEGELKGVL
                     GYCDKPLVSIDYKGDSRSSIIDALSTMVIEDNMVKVVSWYDNEWGYSSRTVDLVKYVA
                     EKLK"
     gene            7200..8369
                     /locus_tag="KR250_003160"
     CDS             7200..8369
                     /locus_tag="KR250_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069054.1"
                     /translation="MEKLTDRRQVKAVSMETFIFLILLVVGFGYVGSIMGAGMMFKVI
                     MSTAHALLLETVFLIMAMAVLAGALSALLSEFGVIALINKIFAVFMKPLYGLPGASIA
                     GAITTYLSDNPAIIPFAKDKTFTQYFKQYQVPALCNLGTAFGMGLILTTFMISQGTEY
                     VLPALIGNLGAIIGSVISVRIMLTFTKKFYKYNPEEDKATGTLEKKDEFREIREGNVF
                     QRALDAILEGGKMGVDMGMAIIPGVLVVCTLVMLLTFGPSTDPVTGQEVYTGAAYEGI
                     KLLPVIGDKLGFILEPLFGFTSPEAIAFPITSLGAVGAAMSLVPEFIKSGAITPNDIA
                     VFTAMGMCWSGYLSTHIGMMDALNARQLAGKAILSHTIGGLCAGAAAHFIFTLVG"
     gene            8749..13002
                     /locus_tag="KR250_003161"
     CDS             8749..13002
                     /locus_tag="KR250_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HBH0069055.1"
                     /translation="MDELFHIGIDVGSTTVKVVVLNNSNNIVHKEYRRHYSDVKKSVK
                     EVLNGIYEKLGDINTTIIITGSGGIGISKKLGVKFVQEVISSTKSIEYFHPETDVVIE
                     LGGEDAKITYLSGGIDQRMNGICAGGTGAFIDQMASLLKTDASGLNELAKGYNVIYPI
                     ASRCGVFAKTDIQPLINDGANQTDIAMSIFNAVVVQTVSVLSCGRKIEGNVAFLGGPL
                     YFLSELREAFKRVLDLKDENIIFPENAQLYIAIGAGLLSVDEDNIQLKSLIEKLDSIK
                     NIEDGEVNLLEPLFKDKNEYEEFSRRHEKEKINYVDINSIKSNCYLGIDAGSTTTKAA
                     LIDEDGRLVYSYYNSNEGNPLKTTIKVINEVYDILPKNIKILSSTVTGYGEGLIKKAL
                     KIDNGEIETIAHYKAAKFFNKDVDFILDIGGQDMKCLKIKDGVIDNIILNEACSSGCG
                     SFLETFASSLSMTIEEFAHEGIYSQNPVDLGSRCTVFMNSRVKQAQKEGASVGDISAG
                     LSYSVIKNALFKVIKIRDLNEIGNNIVVQGGTFYNDLVLRSFEKLIGKNVIRPNISGI
                     MGAFGSALIAKEKYESGYKTSLLSKEELNNIKLEASVTRCKGCSNHCLLTINKFSDNE
                     IFISGNRCEKGEAIYGDKKIGLEKKKPINLFKYKYNRIFRYKPLEKEEAKNGEIGIPR
                     VLNMYEDYPFWFTFFNAMGFRVVLSDRSSKQLYETGITSIASETVCYPGKLVHGHIEN
                     LIAKGIKNIFYPSVTNENKEDSNADNYYNCPVVISYSEVIKNNVENIRNKNINYINPF
                     ISLNDKEKLKKRLYDELSRHFSDIKITKEEVNHAVDKATDEQNLFKMEIQAAGERALK
                     EIKERNMKGIVLCGRPYHIDPEINHGMPELINSLDMAVLTEDSICHLANVQRPLRVVD
                     QWVYHSRLYKAASFVRDKNYLELVQLNSFGCGLDAVTTDQVQEILNEKSKIYTIIKID
                     EGNNLGAAKIRMRSLKAAMFERESKNIDVKNIKVNRIQYAKNNQITNKHTILAPQMSP
                     IHFQFLEKAVNLSGYNIEILKDTDSSVIEEGLRYVNNDACYPAIIVVGQLITALKSGK
                     YDLNNTSVTITQTGGGCRATNYIGFLRKAIYDAGFKDIPVIALSVNGIEDSGIMDNIS
                     LKLINRLFMSVVYGDLLMKVLYRVRPYEKVTGSANALYEKWVDICKSSLVKAKISVFT
                     RNIKDIVKEFDNLEILDIKKPKVGLVGEILVKFHPIANNNLVDILEREGAEAVVPDLT
                     NFFLSCAFNTIYKHTHLEGSRKSRMIGEAFIYITGIYQRVYKKALDKSERFYAPANIK
                     NVAKSTEPVVSLGNQTGEGWLLTGEMVELLNEGVENIICMQPFGCLPNHIIGKGSIKE
                     LKRLYKNANIIPIDYDPSASEVNQLNRIKLMLSKAFKNI"
     gene            13151..13645
                     /locus_tag="KR250_003162"
     CDS             13151..13645
                     /locus_tag="KR250_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HBH0069056.1"
                     /translation="MSLVYDVYKSKLGNLYILSEDGFIVSIYIGDEKFNNLKNECIKK
                     GIDIKRSKEDLKEAISQIDDYFNGKRKVFNLNIRIEGTEFQKSVYREMLKIPYGETLC
                     YSDIARNINNPKSVRAIGQASKSNKIPIIIPCHRVVGKKDIGGYMGNHSDLKEILLNL
                     ERQS"
     gene            13761..14138
                     /locus_tag="KR250_003163"
     CDS             13761..14138
                     /locus_tag="KR250_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HBH0069057.1"
                     /translation="MCDKVIASKYHEKGFNCAESVIKAYNEEFNTDIPVCLGSGLGSG
                     CGVASLCGAVNASNIIIGYVKGRNHEDESTKAKVYAKDLTTTVRKEYGSELCIDLKKD
                     LVACREIMDFAYDSLKETLKKEL"
     gene            14344..>14746
                     /locus_tag="KR250_003164"
     CDS             14344..>14746
                     /locus_tag="KR250_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufBD protein"
                     /protein_id="HBH0069058.1"
                     /translation="MKNLNLLINKLYSKNHNEAYKTFLFLENESLKSNITYCFFDSFL
                     EMIDNENSYIRTRGLLLISANAQWDIDNKIEINIDSILSHIVDKKPSVSRMFIKSIPN
                     ITKYKENLINRIKMELSNADISIYNNNMKPLV"
BASE COUNT         6006 a         1466 c         2598 g         4676 t
ORIGIN      
        1 ataaactact taatttagat aatataaaaa gtcagaattt taagaataga gctatataat
       61 cttagctatt aggagaatta ctatgatagg aatatatttt agtggaacag gaaattcaag
      121 atactgtgta gaaaaatttt tacaagaata tgatataaca gcaaattctt attctattga
      181 agataatgaa cttttacagc acatcaataa tcatgagaat attattttta gttatccggt
      241 gcaatatagt aatgtgccaa aaatattgaa agattttata ataaatcatt caactttgtg
      301 gaatggtaaa cgtgttttta ttattgcaac tatggctcta tttagtggag atggctctgg
      361 tgtgttatct agaatattaa aaaaatacgg tgctataact gttggtggat tacatgtgaa
      421 gatgccagat agtataggag atgaaaaggt attaaaacat tctttagagt ataatagaaa
      481 gcttgtaata aaagcagaaa agaaagtaac taaagctgct aaaaaactga aacatgggtc
      541 accaccaaaa gaagggttag gttttttgag tcatattgta gggttatttg gacaacgatt
      601 gtattttatc aacaaaacaa atatgtatac agataaacta aaaattaata ctgaggaatg
      661 tattgtatgt ggtaaatgtg ttaatttatg tccaatgaag aatctagtaa ttaaaaattg
      721 tatgattgtt gcaaatgggc aatgtacaat gtgttatcgt tgtgtaaata attgtgctaa
      781 aaaagcaatt acattgttag gtaaaaaagt tgttgaacag agtaatatta aaaaatatat
      841 gtaagaaatt taaagtttta ttgaatgtaa attgatgtat attttatgtt agaaagagag
      901 gttaaataat ggaatactac atagttgatt catttgcaac taaactattt aaaggaaatc
      961 ctgctggagt ttgtgttctt gacagaagaa tacctttaga gttaatgcag aaaatagcag
     1021 aagaaaacaa tttaccagaa acagcatttg tagttaaagg taaaggaaat tatgaattac
     1081 gatggtttac tcctaaagca gaaattgatt tatgtggtca tgcaacttta gcagcagctt
     1141 atgtaatatc aaattttatt gatgttaatg taaaaaggat agatttcttt actcaaagtg
     1201 gaaagcttga agttacacga aatggaaatc tatatgaaat gatatttcca gaaataatgc
     1261 ctattgaaat agagttatca ccacaacaag ctaatttgat aggctgtgta ccaagtgctg
     1321 tttattcttc aagagattta atcttattgc taaacagtga acaagaagtt ataaattata
     1381 aacctaatta cgcacaattg cgtaaattga cagactggtt aggtataata atcacagcac
     1441 aaggaagtaa tacagatttt gtatcaagat atttttgtcc agaattagat tcagaagacc
     1501 ctgttactgg ttcatcacat tgtaatctta ttccatactg gtctgaaaag ttgggaaaac
     1561 acaaaatggt agctgctcaa ctttctaatc gtggtggtat aattcagtgt gaagtgttga
     1621 aggataatac tgtgaaaatt tctggtgaag cagtcttgtt tatgcaggga acaataaaga
     1681 ttgatattta aataagtata ataactaatt tattatgaaa aataattaat tttcttagtg
     1741 ttaacaataa taaagtagag ttaaaaaact tgatttcact taaatcaata tgatagatgt
     1801 tgatactaag attgacaagc atgattaaaa aagataatat ttaatctata aagataaaaa
     1861 cacagtctgg ttggtagtcc agacgcagca atagctgtca gtaacctgcc tccttggttg
     1921 tccgttcttt agaaagtaat ttaaggagga actagcaatg gacaaaatta gtggaaaatt
     1981 aacagtctta tttgaagaac cattttgggt aggtatattt gaaagacaag atggtaaaaa
     2041 atatgaagca tgtagggtag tttttggagc agaacctaaa gaagtagaag tgtatgaatt
     2101 tatacttgaa agatttttct cattagattt tggtagcatt aaattagaaa aaaatgtgac
     2161 caaagacaac attggataca aaagaatgca gagaaaagta aaaaaggaac aagaaaaaga
     2221 aactataggg actaaggctc aaaatgctct taagcttcaa tatgaagaaa gaaaacagga
     2281 tagaaaaaac ctagctaaaa atagaaaaga agaagaaaaa gaaagactat ttaatttgaa
     2341 acaagaaaag agaaaagcta aacataaggg tcattaattt taattaaata tctttttaat
     2401 aattgctaga aaaaggtgta taaaattttt aattttatac accttaattt attgaaaaga
     2461 ataaggtttt atatttcaat tactttaaaa tataaaaata tactaataaa aattgtaaaa
     2521 atagtataaa tggtatacca aatgtaaatt taatgtgttt agttttatgt ctaaaagaat
     2581 acattccaat tatagaacct atactacctc caattactgc tatagacata agagtagctt
     2641 cttttattct ccattcgttt ctaattgctc ttttcttatc tatgtacatt gaaaagaaag
     2701 caatagaatt aatcactata aaatatatta gaataaaatt tttcacagtc ttgcctccat
     2761 aaaaatatat tatacattat aaaacaagat atttgttttg gaaagttaaa tttgtaatta
     2821 gtacatataa aatatttgat aagaacaaat ataatattaa ttggaaaatt taaaaaaatt
     2881 aaacaattag caaatagggg gtatatttat aagacacaaa gtgataaaat ctatttagaa
     2941 aataatgtaa aataacagga ggataaagca aaatgaaatt tatagtagaa aaagaagttt
     3001 ttgataaatt agaaaatgta tgctttggtg tagtagttgc aaaaggaata gataatacta
     3061 aagaaataga aagaataagc aaccttttag atataagtat agatagagta gaagattact
     3121 tcaaagataa aaaagtaaag gaatcagaag aaattatacc atatagagag gcatttagaa
     3181 gtctaggcat gaacccaaat aaatttatga gttcgataga agcaatgaca acaagggttg
     3241 ctaagaataa gaaacttcct catataaatc ctattgtaga tttaggaaac tctatttctt
     3301 taaaatattt acttccaatg ggtgctcatg atatggattt tagaaatgat gatgtatatg
     3361 ttagattttc taaaaaaggg gataaatttg taccatttgg agaaactgat gttgaattaa
     3421 tggaagaagg agaattaatt tattcagttg gagacatggt aaaaactaga agatggatat
     3481 ggagacaagg agaagaagga aaaattacta atagctcaaa aaatatattt tttcctatag
     3541 atggttttac tgatgctaat ttagataagg taatgagtgc aagagaagaa ttagcaaaat
     3601 tgttaaagga aatatttaat tgtgaaatta aagtaggttt tgtagataaa gataatcctg
     3661 aaatggaaat ataataagtt gttgaatact tgttaagata ttatatttac ttaatgatgt
     3721 taaaaaggta ttaaaaaaga aaggtcgtag taaatttgaa aaaaactata ataaagaatg
     3781 taaatccaat gattgaaaat aagtttatag gactttttga aatagagtat aaaaacaaat
     3841 tagatgaaga taaggtttgg atggtagcat caagaaaaag tagtgagcaa ttaaaaaata
     3901 tttatttgaa gaatgaagaa gatagtgtag atgcagttgt aatagttggg cttcataaaa
     3961 gtagtaaaaa attaatttta ataagacaat ttagagtacc tattaatgga tatatatacg
     4021 agttaccagc aggtcttgtt gatgaaggag agtctataga tatttccgtt gaaagagaac
     4081 ttagagaaga aacaggactt actcttttag aaattaaaaa aaataaaagt attgataaag
     4141 tttatttatc tcctggaatg agtgatgaat ctgttgcctt tgtttattgt atatgtgatg
     4201 gtgatataac agatgaattt ttagaaccag atgaagagat tgaagcgctt ttaatttcgc
     4261 aagaagatgc aaaagagatt ttacaaagta atcataagat agacacaaaa gcatttttga
     4321 ttttacaaat gtttgtgagt ttaggagcta aattatttga atagatatat agaaaagagg
     4381 ttatatagaa gagtaaaata ataaagttta ttttacttgt gtaaattata atctcttttt
     4441 ttatacaata aagtataatt ttgtgtattt ttaaataagt gatttgactc ttaaattttc
     4501 ataatatgtt ataaaccaat ttaagatact acaataatta aaattccaaa attatttaac
     4561 atatttgcaa ttaaaatgag aaaatgtaaa ggctgaatga ttgaaaaatt tgatgtaaaa
     4621 tagtagctta gtttaatact atagtttgta gggttaaaaa ttagaaattg taagggagat
     4681 aaattctatg aaaaaattag ttattatttt aattagtatc attagtatgg ttagcttgac
     4741 agcttgtaaa aaaagcaatg aaagtaaata taataattta aacaacaaag aatccggaca
     4801 aacaaccatt gaaatggact tagataaaaa ttatgatact tcagacccat ttgttaatgc
     4861 acgtttgttt tgtgtgtcta atgatataga tgttttagac acagagattt cttttaaaat
     4921 ggatggagat agtggtattg tggaaataaa ggacaataaa acagatgaaa ctttatggag
     4981 taatacatgg catggaagcg ttgataatga tacattcaca atttctcttt ctaatataca
     5041 aaaagagaaa gagtatgcta tatgctttac tggaattaaa ataaatcatg cagttgtaaa
     5101 ggttactttt gaaagcaatt tagttaagga gaaagaaaaa ccctcaaaat aaattccagc
     5161 ttttctataa cttgatattc agtacataaa agcagttgat atttatgatt gactgctttt
     5221 tcttatcaaa aaggataggt tgtgcatttt tacttgaaat agtttatgca catcaaaagt
     5281 tggtgagcat gatattggaa atggtaagca tgaaatcttg ataatacaag gaaataaatt
     5341 taagttttca attaagtata tttccataat ttagttaaca ataagctaat tattatttat
     5401 attttgatat ataaataata attgtagcaa attttaaaaa atcatatatt ttttgaacta
     5461 aataaaaatt tatttttatt attttaaatt agaggagatt cttgaagata tattattttt
     5521 aaaaataagt atagtttaaa aattcaaaaa aaagatggca aaatatgtcc caagggacat
     5581 tgagtaaaat aaatgatttc agcttattta cactaaaaag tacatactaa atgaggggta
     5641 aaataaagaa aaatgatata ctaattgtct tttgataata aaaaaacatg gaatttgatt
     5701 atttaatcaa gaaaaagtat tgaacaaatg atttatttag tgtacaataa tcatgtaaaa
     5761 acatagtaat ggagggagac atttcataat gaaaattaag gtaggaatga acggatttgg
     5821 aagaatagga agagctgtat taagaatagc tcaagaagaa ttaggagata atatagaaat
     5881 tgtagccata aatgcaagag caacaacaga atctttggca catttattta catatgattc
     5941 atgctatgga acttttagag gtgaagtaga ggcaaaggat gaagacactc tagtagtaaa
     6001 ctctaaagag ataaaaatat taagatataa tgacccagaa gaattacctt ggaaagaatt
     6061 gggggttgat atagtaatag aatcaacagg attatttaca caaagagaaa aggcagaaaa
     6121 acatattaaa gcaggagcaa aaaaagtaat tataacagct ccaggaaaaa atgaagatat
     6181 aactatagtt atcggagtta atgagaaaga atatgataat gaaaagcaca atataatttc
     6241 aaatgcatct tgtactacaa attgtctagc tccttttgct aaagtattgg acgaaaaatt
     6301 tggtatagta aaagggttaa tgactacagt acattcatat actaacgatc aaagaatatt
     6361 agataaaagc cataaagatt tgagaagagc aagagcagcg gctgaatcta taatacctac
     6421 cactacagga gcagcaaaag ctgtgtcaag agtactacca caattagaag gaaaattaaa
     6481 tggtttttca cttagagtac caacaccaac agtttcactt gttgatttag tgtgcgaact
     6541 taaagaaaac gttacatctg agcaagttaa ctctgtgtta aaagatgctg ctgaaggaga
     6601 attaaaaggt gttttaggat attgtgacaa gccacttgtt tctatagatt ataaaggaga
     6661 ttcaagatca tctattatag acgctctatc cacaatggtt atagaagaca atatggttaa
     6721 ggtagtttct tggtatgata atgaatgggg atattcttca agaactgttg acttagtaaa
     6781 atatgtagct gaaaaattaa aataatatat atttcttaaa gcaaatttta aaaaaatttt
     6841 ttaaaaaaat tatataaaac taaaaaaaaa gattatgaaa aatattgaag gaaaaatttt
     6901 ttcataatct tttttctttc atgcaaaaag atacaagtta caacttatat ttgcatatta
     6961 ggtcgttaga gaagtatata tatatttagt aaggaatcat actttttata aaatgaaaga
     7021 taagatgtcg aaaacttgca taaatctgaa aaatttttat tattaaaata ctgatttcca
     7081 ttttaaaaat gataaaaaaa tgacaaataa agttgcaaat agtaatactt ataagtataa
     7141 ttaaaaaaga gaaaaggttt tgttaaactt tataacaaaa aaagaaggga tgattcaaaa
     7201 tggagaagtt aacagacagg agacaggtta aggcagtaag catggaaacc ttcattttcc
     7261 taatattact agtagttggt tttgggtatg ttggtagtat tatgggagca ggaatgatgt
     7321 tcaaagtaat aatgagtaca gcacatgctt tactattgga gacagttttc ttaattatgg
     7381 ctatggcagt tcttgcaggc gcattaagcg cactactttc agagtttgga gtaatagcgt
     7441 taattaataa aattttcgct gtatttatga agcctttata tggactacct ggtgctagta
     7501 tagcaggagc tataacaact tatttatcag ataatccagc tattatacct tttgctaaag
     7561 ataaaacatt cacacagtat tttaaacaat accaagttcc agcattatgt aacttaggaa
     7621 cagcctttgg tatgggactt atcttaacaa catttatgat ttctcaaggt acagaatatg
     7681 tacttccagc attaattggt aatttaggtg caattatagg tagtgtcata agtgttagaa
     7741 taatgttgac atttactaag aagttttata aatacaatcc ggaagaagat aaagcaacag
     7801 gaacattaga gaaaaaggat gaatttagag aaatcagaga aggaaatgta tttcaaagag
     7861 ctttagatgc aattcttgaa ggtggtaaaa tgggggtaga tatgggaatg gctataattc
     7921 ctggagtcct agtagtatgt acattggtga tgttattaac ttttggacca tctacagatc
     7981 ctgttacagg tcaagaagtt tatacaggag ctgcatatga aggaataaaa ttacttccag
     8041 ttattggaga taaacttgga tttatattag aaccattatt tggatttaca tcaccagagg
     8101 caatagcatt ccctataact tcattgggag ctgttggcgc agcaatgtct ctagttccag
     8161 agtttataaa gtcaggagct ataactccta atgatatagc agtatttaca gctatgggta
     8221 tgtgttggag tggatattta agtacacata ttggtatgat ggatgcatta aatgctagac
     8281 agttggcagg taaagctatt ctttctcata caataggtgg tttatgtgca ggagcagctg
     8341 ctcactttat atttacactc gtaggataaa tgtaatcagc taagataaaa aataatatac
     8401 aaaaaatggt tgttgatatg taataagaat ttacatatca acaaccattt ttttctgact
     8461 aaagataatt tataatacat aaacattaag tggatattag agttaagtta tatttatgta
     8521 aaaagtaaca aaaatctgta gaaatttatg acaaaattta gtacactaac tatatgttct
     8581 tatttaaaat ttaaggttag aattaggaat tacctagtag agtaagcttt gattaataaa
     8641 agaatcataa aataaaaata ttagtcataa agattataaa taacttactt aaaataaagc
     8701 catagaactt acaaaaatta aaaaagcata taaatatgga ggggtacaat ggatgaatta
     8761 tttcatatag gaattgatgt aggttctaca acggtgaagg ttgtggtact taataatagt
     8821 aataatatag ttcataaaga atatagaaga cattattctg atgtaaaaaa atcagttaaa
     8881 gaagtactaa atggtatata tgaaaaattg ggagatataa atactaccat tataataact
     8941 ggttcaggtg gaattggaat atcaaaaaaa ttaggtgtaa aatttgttca ggaagttata
     9001 tctagtacaa aatcaataga gtattttcat cctgaaactg atgttgtcat agaacttggg
     9061 ggtgaagatg caaagataac atatctaagt ggaggaatag accaaagaat gaatggaata
     9121 tgtgcaggtg gcactggagc attcatagac cagatggcat cacttttaaa gacagatgcc
     9181 agtggattaa atgaacttgc taaagggtat aatgttatat atcccatagc ttcaagatgt
     9241 ggtgtttttg ctaaaacaga tattcaacct cttataaatg atggagccaa tcagactgat
     9301 atagcaatga gtattttcaa tgcagttgtc gtacaaactg tgagtgtcct ttcttgtgga
     9361 agaaaaatag aaggaaatgt tgcgtttttg ggtggtccat tgtacttttt atctgaactt
     9421 agagaagcat ttaaaagagt attagattta aaagatgaaa acataatctt cccagaaaat
     9481 gcacaacttt atattgctat tggagcagga ttattatcag tagatgaaga taatatacaa
     9541 ctaaaatctc taatagaaaa attggatagt ataaagaata ttgaagatgg agaagtaaat
     9601 ttattagaac ctttatttaa agataaaaat gagtatgaag aattttcaag aagacatgaa
     9661 aaagaaaaaa taaattatgt ggacataaat tcaataaaaa gcaattgtta tttaggaatt
     9721 gatgcaggct caactacaac taaagcagca cttattgatg aggatggaag acttgtatat
     9781 tcttattata atagcaatga aggaaatcca ttaaaaacaa ccattaaagt tataaatgaa
     9841 gtatatgata tattaccaaa aaatattaaa atattgagtt caactgtaac tggatatgga
     9901 gaaggtctta ttaaaaaagc tcttaaaatc gataatggag agatagagac tatagcacat
     9961 tacaaagctg ctaaattttt caacaaagat gtagatttta tacttgatat tggtggtcaa
    10021 gatatgaagt gcctaaaaat aaaagatgga gtaattgata atataatatt aaatgaggct
    10081 tgttcatcag gttgtggctc atttttagag acatttgcaa gttctttatc tatgacaata
    10141 gaagaatttg cgcatgaagg tatttattct caaaatcctg tagatttagg ttcaagatgt
    10201 actgtattta tgaattctag agtaaaacaa gctcaaaaag aaggtgcaag tgttggagat
    10261 atatcagcag ggctatctta ttcagtaata aaaaatgctt tatttaaagt aataaagatt
    10321 agagatttaa atgagattgg taataatatt gttgtacaag gtggaacctt ttataatgat
    10381 ttggtgctta gaagctttga aaaactaatt ggaaaaaatg taattagacc taatatttca
    10441 ggaattatgg gagcttttgg ttcagcactt atagctaaag aaaaatatga aagtggatac
    10501 aaaacatcct tgttatctaa ggaagagttg aataatatca agctagaagc aagtgtaact
    10561 agatgtaaag gttgttcaaa tcattgtctt cttacaataa ataaattttc tgataatgaa
    10621 atttttattt caggtaatag atgtgagaaa ggtgaagcta tttatggtga taaaaaaata
    10681 ggattagaaa agaaaaaacc aataaattta tttaagtaca aatacaatag aatttttaga
    10741 tacaaaccat tggaaaaaga agaagcaaaa aatggggaaa tagggatacc aagagtatta
    10801 aatatgtatg aagattatcc attttggttt acttttttca atgctatggg ctttagagtt
    10861 gtactttcag ataggtcatc aaaacagtta tatgaaactg gtataactag tattgcatct
    10921 gaaacagttt gttatccagg aaaattagtt catggacata tagaaaattt aattgcaaaa
    10981 ggtataaaaa atatatttta tccaagtgta acaaatgaaa ataaagagga ttcaaatgca
    11041 gataattatt ataattgtcc tgttgtaata tcatattctg aagtaataaa aaacaatgta
    11101 gagaatataa gaaataagaa cataaattat ataaatccat ttataagtct aaatgacaag
    11161 gaaaaattga agaaaagact ctatgatgaa ttatcaagac atttttctga tataaaaata
    11221 actaaagaag aagttaatca tgcagttgat aaagctactg atgaacagaa tttatttaaa
    11281 atggagatac aagcagcagg tgaaagagca cttaaagaaa taaaagaaag aaatatgaag
    11341 gggattgtgt tatgtggaag accatatcat atagacccag aaataaatca tggaatgcca
    11401 gagcttataa attcattaga tatggcagtg cttactgaag actctatatg tcatttagcc
    11461 aatgtacaaa gaccacttag agttgtagac caatgggtat atcattcaag attgtataaa
    11521 gcagctagtt ttgtaagaga taaaaactat ttggaacttg ttcaacttaa ttcatttggc
    11581 tgtggattag atgcagtaac tacagaccag gttcaagaaa tacttaatga aaaatcgaag
    11641 atatatacta ttataaagat tgacgaaggc aacaatttag gagcagctaa gataaggatg
    11701 aggtccttaa aagcagctat gtttgagaga gaaagcaaaa atatagatgt aaaaaatata
    11761 aaggtaaaca ggatacagta tgctaaaaat aatcagataa cgaacaaaca tactatatta
    11821 gcaccacaaa tgtctccaat acatttccaa ttccttgaaa aagctgttaa cttaagtggt
    11881 tataatatag aaattctaaa agatactgat agtagtgtaa ttgaagaagg gttaagatat
    11941 gtaaataatg atgcatgtta tccagctata atagttgtag gtcagctaat aacagcatta
    12001 aaatctggca aatatgattt aaataacact tctgttacta taactcagac tggtggaggt
    12061 tgtagagcta caaattatat tggattctta agaaaggcta tatacgatgc tggatttaaa
    12121 gatattcctg tcattgcact tagtgtaaat ggaatagaag atagtggtat aatggataat
    12181 atatcactaa aattaatcaa tagactcttt atgtctgttg tttatggtga tttgctgatg
    12241 aaagtactat atagagtaag accatatgaa aaagttactg gaagtgcaaa tgctctatat
    12301 gaaaaatggg ttgatatatg caaatcttct cttgttaaag caaaaatttc tgtatttact
    12361 agaaatataa aagatatagt aaaagaattt gataatttag agatactaga tatcaaaaaa
    12421 ccaaaagtag gtttggttgg agaaatattg gtgaaattcc atccaattgc aaataataat
    12481 ttagtagata tattagagcg agaaggtgct gaagcagtcg tgccagacct tacaaatttc
    12541 ttcttaagtt gtgcctttaa tactatatac aaacacactc atttagaagg aagtagaaag
    12601 agtagaatga taggagaagc atttatatac ataactggaa tatatcaaag agtatataaa
    12661 aaagcacttg ataagagtga gagattttat gctccagcca atataaaaaa tgttgctaaa
    12721 agtacagaac cagtagtgtc attaggtaat caaactggag aaggttggct tttaacaggt
    12781 gagatggttg aattattaaa tgaaggtgta gaaaacatta tttgtatgca accttttgga
    12841 tgtttaccta atcacataat tggtaagggt tctataaaag aattaaagag gttgtataaa
    12901 aatgcaaata taatacctat tgattatgac ccttctgcaa gtgaggtaaa tcaacttaac
    12961 aggataaaac ttatgctttc taaggctttt aaaaatatat aagatttata ttttgatatg
    13021 ttaaaagtta atgaatttaa aaatatactc tatattaagt atctataaaa aagagttatc
    13081 ctatttttta gggtaactct ttattagtta aaaaattata tgtatactga tattaaaggg
    13141 gggaagtaat atgagtttag tatatgatgt ttataaatca aaattaggaa atttgtacat
    13201 attaagtgaa gatggtttta tagttagtat atacatagga gatgaaaaat ttaataattt
    13261 aaaaaatgaa tgtattaaaa aaggaattga tataaagaga agtaaggaag atttaaagga
    13321 agcaataagc cagatagatg attactttaa tggaaaaaga aaagtcttta atttaaatat
    13381 tcgtattgaa gggactgaat ttcaaaaatc agtttatagg gaaatgttaa aaatacctta
    13441 tggagaaaca ttgtgttatt cagatatagc aagaaatata aacaatccaa aatcagtaag
    13501 agcaatagga caagcaagta aaagtaataa aattccaata ataatacctt gtcatagagt
    13561 agttggcaaa aaagatattg gtggatatat ggggaatcat tctgatttga aagagatact
    13621 attaaattta gaaagacaga gttaacttag aaagaaatgt ttttattgtt aatatttgat
    13681 ataataatag taaaaagaag tattttttac aaaaacaatg aagaatttat tttaatttat
    13741 aaaaatgagg gggaatttta atgtgtgata aagttatagc atcaaaatat catgagaagg
    13801 gatttaattg tgcagaatca gttataaaag catataatga ggaatttaat acagatatac
    13861 cagtttgttt aggaagtgga ctgggttcag ggtgtggggt tgctagttta tgtggtgcag
    13921 taaatgcatc aaatataata attggatatg taaaaggcag aaaccacgaa gatgaaagta
    13981 ctaaagctaa agtatatgct aaagatttaa ctacaacagt aagaaaagag tatggttcag
    14041 agttatgtat agacctaaaa aaagatttag ttgcttgtag agaaataatg gatttcgcat
    14101 atgatagtct aaaggaaact ttaaaaaaag aattataaga aaataagcta tcaagtataa
    14161 attatttgat agcttatttt tatagtcgat actaaataaa tcaaatatat tttagaatta
    14221 ttatttgtaa ataaacagca aataatttta ggatttttat ttaatatagt ttttatttat
    14281 atttattttt gaatatgtta tactttgttt aaataaatat ttgtaatgag ggagatgaga
    14341 agattgaaga atctcaattt acttattaat aaattatatt caaaaaatca taatgaagca
    14401 tataaaactt ttttattctt agaaaatgaa agtttaaaat caaatattac atattgtttt
    14461 tttgactcat tcctagaaat gattgataac gaaaactcat atattcgaac aaggggattg
    14521 ttattaatct ctgcaaatgc tcagtgggat attgataata aaattgaaat aaatatagat
    14581 agtatactaa gtcatatcgt agataaaaaa ccttctgtat caagaatgtt tattaaatcc
    14641 atacctaata ttacaaagta taaagaaaat cttattaatc gcataaaaat ggaattaagt
    14701 aatgcagata taagtattta taataataat atgaaaccat tagtag
//
LOCUS       DAEPIN010000085        14137 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.085, whole genome shotgun sequence.
ACCESSION   DAEPIN010000085 DAEPIN010000000
VERSION     DAEPIN010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14137
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.085"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            <1..128
                     /locus_tag="KR250_003165"
     CDS             <1..128
                     /locus_tag="KR250_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBH0069059.1"
                     /translation="LKGYSDREISVKLKISRQAVNKAKNRAFKKIKKDYENYFNL"
     gene            446..7546
                     /gene="tcdB"
                     /locus_tag="KR250_003166"
                     /gene_synonym="toxB"
     CDS             446..7546
                     /gene="tcdB"
                     /locus_tag="KR250_003166"
                     /gene_synonym="toxB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_074049122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin TcdB"
                     /protein_id="HBH0069060.1"
                     /translation="MSLVNRKQLEKMANVRFRTQEDEYVAILDALEEYHNMSENTVVE
                     KYLKLKDINSLTDIYIDTYKKSGRNKALKKFKEYLVTEVLELKNNNLTPVEKNLHFVW
                     IGGQINDTAINYINQWKDVNSDYNVNVFYDSNAFLINTLKKTVVESAINDTLESFREN
                     LNDPRFDYNKFFRKRMEIIYDKQKNFINYYKAQREENPELIIDDIVKTYLSNEYSKEI
                     DELNTYIEESLNKITQNSGNDVRNFEEFKNGESFNLYEQELVERWNLAAASDILRISA
                     LKEIGGMYLDVDMLPGIQPDLFESIEKPSSVTVDFWEMTKLEAIMKYKEYIPEYTSEH
                     FDMLDEEVQSSFESVLASKSDKSEIFSSLGDMEASPLEVKIAFNSKGIINQGLISVKD
                     SYCSNLIVKQIENRYKILNNSLNPAISEDNDFNTTTNTFIDSIMAEANADNGRFMMEL
                     GKYLRVGFFPDVKTTINLSGPEAYAAAYQDLLMFKEGSMNIHLIEADLRNFEISKTNI
                     SQSTEQEMASLWSFDDARAKAQFEEYKRNYFEGSLGEDDNLDFSQNIVVDKEYLLEKI
                     SSLARSSERGYIHYIVQLQGDKISYEAACNLFAKTPYDSVLFQKNIEDSEIAYYYNPG
                     DGEIQEIDKYKIPSIISDRPKIKLTFIGHGKDEFNTDIFAGFDVDSLSTEIEAAIDLA
                     KEDISPKSIEINLLGCNMFSYSINVEETYPGKLLLKVKDKISELMPSISQDSIIVSAN
                     QYEVRINSEGRRELLDHSGEWINKEESIIKDISSKEYISFNPKENKITVKSKNLPELS
                     TLLQEIRNNSNSSDIELEEKVMLTECEINVISNIDTQIVEERIEEAKNLTSDSINYIK
                     DEFKLIESISDALCDLKQQNELEDSHFISFEDISETDEGFSIRFINKETGESIFVETE
                     KTIFSEYANHITEEISKIKGTIFDTVNGKLVKKVNLDTTHEVNTLNAAFFIQSLIEYN
                     SSKESLSNLSVAMKVQVYAQLFSTGLNTITDAAKVVELVSTALDETIDLLPTLSEGLP
                     IIATIIDGVSLGAAIKELSETSDPLLRQEIEAKIGIMAVNLTTATTAIITSSLGVASG
                     FSILLVPLAGISAGIPSLVNNELVLRDKATKVVDYFKHVSLVETEGVFTLLDDKIMMP
                     QDDLVISEIDFNNNSIVLGKCEIWRMEGGSGHTVTDDIDHFFSAPSITYREPHLSIYD
                     VLEVQKEELDLSKDLMVLPNAPNRVFAWETGWTPGLRSLENDGTKLLDRIRDNYEGEF
                     YWRYFAFIADALITTLKPRYEDTNIRINLDSNTRSFIVPIITTEYIREKLSYSFYGSG
                     GTYALSLSQYNMGINIELSESDVWIIDVDNVVRDVTIESDKIKKGDLIEGILSTLSIE
                     ENKIILNSHEINFSGEVNGSNGFVSLTFSILEGINAIIEVDLLSKSYKLLISGELKIL
                     MLNSNHIQQKIDYIGFNSELQKNIPYSFVDSEGKENGFINGSTKEGLFVSELPDVVLI
                     SKVYMDDSKPSFGYYSNNLKDVKVITKDNVNILTGYYLKDDIKISLSLTLQDEKTIKL
                     NSVHLDESGVAEILKFMNRKGNTNTSDSLMSFLESMNIKSIFVNFLQSNIKFILDANF
                     IISGTTSIGQFEFICDENDNIQPYFIKFNTLETNYTLYVGNRQNMIVEPNYDLDDSGD
                     ISSTVINFSQKYLYGIDSCVNKVVISPNIYTDEINITPVYETNNTYPEVIVLDANYIN
                     EKINVNINDLSIRYVWSNDGNDFILMSTSEENKVSQVKIRFVNVFKDKTLANKLSFNF
                     SDKQDVPVSEIILSFTPSYYEDGLIGYDLGLVSLYNEKFYINNFGMMVSGLIYINDSL
                     YYFKPPVNNLITGFVTVGDDKYYFNPINGGAASIGETIIDDKNYYFNQSGVLQTGVFS
                     TEDGFKYFAPANTLDENLEGEAIDFTGKLIIDENIYYFDDNYRGAVEWKELDGEMHYF
                     SPETGKAFKGLNQIGDYKYYFNSDGVMQKGFVSINDNKHYFDDSGVMKVGYTEIDGKH
                     FYFAENGEMQIGVFNTEDGFKYFAHHNEDLGNEEGEEISYSGILNFNNKIYYFDDSFT
                     AVVGWKDLEDGSKYYFDEDTAEAYIGLSLINDGQYYFNDDGIMQVGFVTINDKVFYFS
                     DSGIIESGVQNIDDNYFYIDDNGIVQIGVFDTSDGYKYFAPANTVNDNIYGQAVEYSG
                     LVRVGEDVYYFGETYTIETGWIYDMENESDKYYFNPETKKACKGINLIDDIKYYFDEK
                     GIMRTGLISFENNNYYFNENGEMQFGYINIEDKMFYFGEDGVMQIGVFNTPDGFKYFA
                     HQNTLDENFEGESINYTGWLDLDEKRYYFTDEYIAATGSVIIDGEEYYFDPDTAQLVI
                     SE"
     gene            7668..8168
                     /gene="tcdE"
                     /locus_tag="KR250_003167"
     CDS             7668..8168
                     /gene="tcdE"
                     /locus_tag="KR250_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin-like glycosylating toxin export protein
                     TcdE"
                     /protein_id="HBH0069061.1"
                     /translation="MHSSSPFYISNGNKIFFYINLGGVMNMTISFLSEHIFIKLVILT
                     ISFDTLLGCLSAIKSRKFNSSFGIDGGIRKVAMIACIFFLSVVDILTKFNFLFMLPQD
                     CINFLRLKHLGISEFFSILFILYESVSILKNMCLCGLPVPKRLKEKIAILLDAMTDEM
                     NAKDEK"
     gene            8497..8679
                     /locus_tag="KR250_003168"
     CDS             8497..8679
                     /locus_tag="KR250_003168"
                     /inference="COORDINATES: protein motif:HMM:NF022413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069062.1"
                     /translation="MRDEGQNDIENKYQTLINDLIDSLKVIGKIYKEIKTLKKYKICL
                     QITIRQSPYLIEESIN"
     gene            8896..>14137
                     /gene="tcdA"
                     /locus_tag="KR250_003169"
                     /gene_synonym="toxA"
     CDS             8896..>14137
                     /gene="tcdA"
                     /locus_tag="KR250_003169"
                     /gene_synonym="toxA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021414120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin TcdA"
                     /protein_id="HBH0069063.1"
                     /translation="MSLISKEELIKLAYSIRPRENEYKTILTNLDEYNKLTTNNNENK
                     YLQLKKLNESIDVFMNKYKTSSRNRALSNLKKDILKEVILIKNSNTSPVEKNLHFVWI
                     GGEVSDIALEYIKQWADINAEYNIKLWYDSEAFLVNTLKKAIVESSTTEALQLLEEEI
                     QNPQFDNMKFYKKRMEFIYDRQKRFINYYKSQINKPTVPTIDDIIKSHLVSEYNRDET
                     VLESYRTNSLRKINSNHGIDIRANSLFTEQELLNIYSQELLNRGNLAAASDIVRLLAL
                     KNFGGVYLDVDMLPGIHSDLFKTISRPSSIGLDRWEMIKLEAIMKYKKYINNYTSENF
                     DKLDQQLKDNFKLIIESKSEKSEIFSKLENLNVSDLEIKIAFALGSVINQALISKQGS
                     YLTNLVIEQVKNRYQFLNQHLNPAIESDNNFTDTTKIFHDSLFNSATAENSMFLTKIA
                     PYLQVGFMPEARSTISLSGPGAYASAYYDFINLQENTIEKTLKASDLIEFKFPENNLS
                     QLTEQEINSLWSFDQASAKYQFEKYVRDYTGGSLSEDNGVDFNKNTALDKNYLLNNKI
                     PSNNVEEAGSKNYVHYIIQLQGDDISYEATCNLFSKNPKNSIIIQRNMNESAKSYFLS
                     DDGESILELNKYRIPERLKNKEKVKVTFIGHGKDEFNTSEFARLSVDSLSNEISSFLD
                     TIKLDISPKNVEVNLLGCNMFSYDFNVEETYPGKLLLSIMDKITSTLPDVNKNSITIG
                     ANQYEVRINSEGRKELLAHSGKWINKEEAIMSDLSSKEYIFFDSIDNKLKAKSKNIPG
                     LASISEDIKTLLLDASVSPDTKFILNNLKLNIESSIGDYIYYEKLEPVKNIIHNSIDD
                     LIDEFNLLENVSDELYELKKLNNLDEKYLISFEDISKNNSTYSVRFINKSNGESVYVE
                     TEKEIFSKYSEHITKEISTIKNSIITDVNGNLLDNIQLDHTSQVNTLNAAFFIQSLID
                     YSSNKDVLNDLSTSVKVQLYAQLFSTGLNTIYDSIQLVNLISNAVNDTINVLPTITEG
                     IPIVSTILDGINLGAAIKELLDEHDPLLKKELEAKVGVLAINMSLSIAATVASIVGIG
                     AEVTIFLLPIAGISAGIPSLVNNELILHDKATSVVNYFNHLSESKKYGPLKTEDDKIL
                     VPIDDLVISEIDFNNNSIKLGTCNILAMEGGSGHTVTGNIDHFFSSPSISSHIPSLSI
                     YSAIGIETENLDFSKKIMMLPNAPSRVFWWETGAVPGLRSLENDGTRLLDSIRDLYPG
                     KFYWRFYAFFDYAITTLKPVYEDTNIKIKLDKDTRNFIMPTITTNEIRNKLSYSFDGA
                     GGTYSLLLSSYPISTNINLSKDDLWIFNIDNEVREISIENGTIKKGKLIKDVLSKIDI
                     NKNKLIIGNQTIDFSGDIDNKDRYIFLTCELDDKISLIIEINLVAKSYSLLLSGDKNY
                     LISNLSNTIEKINTLGLDSKNIAYNYTDESNNKYFGAISKTSQKSIIHYKKDSKNILE
                     FYNDSTLEFNSKDFIAEDINVFMKDDINTITGKYYVDNNTDKSIDFSISLVSKNQVKV
                     NGLYLNESVYSSYLDFVKNSDGHHNTSNFMNLFLDNISFWKLFGFENINFVIDKYFTL
                     VGKTNLGYVEFICDNNKNIDIYFGEWKTSSSKSTIFSGNGRNVVVEPIYNPDTGEDIS
                     TSLDFSYEPLYGIDRYINKVLIAPDLYTSLININTNYYSNEYYPEIIVLNPNTFHKKV
                     NINLDSSSFEYKWSTEGSDFI"
BASE COUNT         5719 a         1487 c         2239 g         4692 t
ORIGIN      
        1 atttaaaagg atattctgat agagaaatat cagtgaaatt aaaaatatct agacaagctg
       61 ttaataaggc taaaaataga gcatttaaaa aaataaaaaa agactatgaa aattatttta
      121 acttgtaatt aatgagctta aagaaatatt tacaatagaa atcaaatttt agaattaact
      181 ttattgtaaa atcaataact taatctaaga atatcttaat ttttatattt tatatagaac
      241 aaagtttaca tatttatttc agacaacgtc tttattcaat cgaagagcaa attaatcaac
      301 tgagtgtctt caatttaaaa tgttaggaag tgaatgtata tgaaaaccta agtagatatt
      361 agtatatttt ataaatagaa aggaggatat ataaaagagt tttatcattt agatgtaaaa
      421 atattatagt aaaggagaaa attttatgag tttagttaat agaaaacagt tagaaaaaat
      481 ggcaaatgta agatttcgta ctcaagaaga tgaatatgtt gcaatattgg atgctttaga
      541 agaatatcat aatatgtcag agaatactgt agtcgaaaaa tatttaaaat taaaagatat
      601 aaatagttta acagatattt atatagatac atataaaaaa tctggtagaa ataaagcctt
      661 aaaaaaattt aaggaatatc tagttacaga agtattagag ctaaagaata ataatttaac
      721 tccagttgag aaaaatttac attttgtttg gattggaggt caaataaatg acactgctat
      781 taattatata aatcaatgga aagatgtaaa tagtgattat aatgttaatg ttttttatga
      841 tagtaatgca tttttgataa acacattgaa aaaaactgta gtagaatcag caataaatga
      901 tacacttgaa tcatttagag aaaacttaaa tgaccctaga tttgactata ataaattctt
      961 cagaaaacgt atggaaataa tttatgataa acagaaaaat ttcataaact actataaagc
     1021 tcaaagagaa gaaaatcctg aacttataat tgatgatatt gtaaagacat atctttcaaa
     1081 tgagtattca aaggagatag atgaacttaa tacctatatt gaagaatcct taaataaaat
     1141 tacacagaat agtggaaatg atgttagaaa ctttgaagaa tttaaaaatg gagagtcatt
     1201 caacttatat gaacaagagt tggtagaaag gtggaattta gctgctgctt ctgacatatt
     1261 aagaatatct gcattaaaag aaattggtgg tatgtattta gatgttgata tgttaccagg
     1321 aatacaacca gacttatttg agtctataga gaaacctagt tcagtaacag tggatttttg
     1381 ggaaatgaca aagttagaag ctataatgaa atacaaagaa tatataccag aatatacctc
     1441 agaacatttt gacatgttag acgaagaagt tcaaagtagt tttgaatctg ttctagcttc
     1501 taagtcagat aaatcagaaa tattctcatc acttggtgat atggaggcat caccactaga
     1561 agttaaaatt gcatttaata gtaagggtat tataaatcaa gggctaattt ctgtgaaaga
     1621 ctcatattgt agcaatttaa tagtaaaaca aatcgagaat agatataaaa tattgaataa
     1681 tagtttaaat ccagctatta gcgaggataa tgattttaat actacaacga atacctttat
     1741 tgatagtata atggctgaag ctaatgcaga taatggtaga tttatgatgg aactaggaaa
     1801 gtatttaaga gttggtttct tcccagatgt taaaactact attaacttaa gtggccctga
     1861 agcatatgcg gcagcttatc aagatttatt aatgtttaaa gaaggcagta tgaatatcca
     1921 tttgatagaa gctgatttaa gaaactttga aatctctaaa actaatattt ctcaatcaac
     1981 tgaacaagaa atggctagct tatggtcatt tgacgatgca agagctaaag ctcaatttga
     2041 agaatataaa aggaattatt ttgaaggttc tcttggtgaa gatgataatc ttgatttttc
     2101 tcaaaatata gtagttgaca aggagtatct tttagaaaaa atatcttcat tagcaagaag
     2161 ttcagagaga ggatatatac actatattgt tcagttacaa ggagataaaa ttagttatga
     2221 agcagcatgt aacttatttg caaagactcc ttatgatagt gtactatttc agaaaaatat
     2281 agaagattca gaaattgcat attattataa tcctggagat ggtgaaatac aagaaataga
     2341 caagtataaa attccaagta taatttctga tagacctaag attaaattaa catttattgg
     2401 tcatggtaaa gatgaattta atactgatat atttgcaggt tttgatgtag attcattatc
     2461 cacagaaata gaagcagcaa tagatttagc taaagaggat atttctccta agtcaataga
     2521 aataaattta ttaggatgta atatgtttag ctactctatc aacgtagagg agacttatcc
     2581 tggaaaatta ttacttaaag ttaaagataa aatatcagaa ttaatgccat ctataagtca
     2641 agactctatt atagtaagtg caaatcaata tgaagttaga ataaatagtg aaggaagaag
     2701 agaattattg gatcattctg gtgaatggat aaataaagaa gaaagtatta taaaggatat
     2761 ttcatcaaaa gaatatatat catttaatcc taaagaaaat aaaattacag taaaatctaa
     2821 aaatttacct gagctatcta cattattaca agaaattaga aataattcta attcaagtga
     2881 tattgaacta gaagaaaaag taatgttaac agaatgtgag ataaatgtta tttcaaatat
     2941 agatacgcaa attgttgagg aaaggattga agaagctaag aatttaactt ctgactctat
     3001 taattatata aaagatgaat ttaaactaat agaatctatt tctgatgcac tatgtgactt
     3061 aaaacaacag aatgaattag aagattctca ttttatatct tttgaggaca tatcagagac
     3121 tgatgaggga tttagtataa gatttattaa taaagaaact ggagaatcta tatttgtaga
     3181 aactgaaaaa acaatattct ctgaatatgc taatcatata actgaagaga tttctaagat
     3241 aaaaggtact atatttgata ctgtaaatgg taagttagta aaaaaagtaa atttagatac
     3301 tacacacgaa gtaaatactt taaatgctgc attttttata caatcattaa tagaatataa
     3361 tagttctaaa gaatctctta gtaatttaag tgtagcaatg aaagtccaag tttacgctca
     3421 attatttagt actggtttaa atactattac agatgcagcc aaagttgttg aattagtatc
     3481 aactgcatta gatgaaacta tagacttact tcctacatta tctgaaggat tacctataat
     3541 tgcaactatt atagatggtg taagtttagg tgcagcaatc aaagagctaa gtgaaacgag
     3601 tgacccatta ttaagacaag aaatagaagc taagataggt ataatggcag taaatttaac
     3661 aacagctaca actgcaatca ttacttcatc tttgggggta gctagtggat ttagtatact
     3721 tttagttcct ttagcaggaa tttcagcagg tataccaagc ttagtaaaca atgaacttgt
     3781 acttcgagat aaggcaacaa aggttgtaga ttattttaaa catgtttcat tagttgaaac
     3841 tgaaggagta tttactttat tagatgataa aataatgatg ccacaagatg atttagtgat
     3901 atcagaaata gattttaata ataattcaat agttttaggt aaatgtgaaa tctggagaat
     3961 ggaaggtggt tcaggtcata ctgtaactga tgatatagat cacttctttt cagcaccatc
     4021 aataacatat agagagccac acttatctat atatgacgta ttggaagtac aaaaagaaga
     4081 acttgatttg tcaaaagatt taatggtatt acctaatgct ccaaatagag tatttgcttg
     4141 ggaaacagga tggacaccag gtttaagaag cttagaaaat gatggcacaa aactgttaga
     4201 ccgtataaga gataactatg aaggtgagtt ttattggaga tattttgctt ttatagctga
     4261 tgctttaata acaacattaa aaccaagata tgaagatact aatataagaa taaatttaga
     4321 tagtaatact agaagtttta tagttccaat aataactaca gaatatataa gagaaaaatt
     4381 atcatattct ttctatggtt caggaggaac ttatgcattg tctctttctc aatataatat
     4441 gggtataaat atagaattaa gtgaaagtga tgtttggatt atagatgttg ataatgttgt
     4501 gagagatgta actatagaat ctgataaaat taaaaaaggt gatttaatag aaggtatttt
     4561 atctacacta agtattgaag agaataaaat tatcttaaat agccatgaga ttaatttttc
     4621 tggtgaggta aatggaagta atggatttgt ttctttaaca ttttcaattt tagaaggaat
     4681 aaatgcaatt atagaagttg atttattatc taaatcatat aaattactta tttctggcga
     4741 attaaaaata ttgatgttaa attcaaatca tattcaacag aaaatagatt atataggatt
     4801 caatagcgaa ttacagaaaa atataccata tagctttgta gatagtgaag gaaaagagaa
     4861 tggttttatt aatggttcaa caaaagaagg tttatttgta tctgaattac ctgatgtagt
     4921 tcttataagt aaggtttata tggatgatag taagccttca tttggatatt atagtaataa
     4981 tttgaaagat gtcaaagtta taactaaaga taatgttaat atattaacag gttattatct
     5041 taaggatgat ataaaaatct ctctttcttt gactctacaa gatgaaaaaa ctataaagtt
     5101 aaatagtgtg catttagatg aaagtggagt agctgagatt ttgaagttca tgaatagaaa
     5161 aggtaataca aatacttcag attctttaat gagcttttta gaaagtatga atataaaaag
     5221 tattttcgtt aatttcttac aatctaatat taagtttata ttagatgcta attttataat
     5281 aagtggtact acttctattg gccaatttga gtttatttgt gatgaaaatg ataatataca
     5341 accatatttc attaagttta atacactaga aactaattat actttatatg taggaaatag
     5401 acaaaatatg atagtggaac caaattatga tttagatgat tctggagata tatcttcaac
     5461 tgttatcaat ttctctcaaa agtatcttta tggaatagac agttgtgtta ataaagttgt
     5521 aatttcacca aatatttata cagatgaaat aaatataacg cctgtatatg aaacaaataa
     5581 tacttatcca gaagttattg tattagatgc aaattatata aatgaaaaaa taaatgttaa
     5641 tatcaatgat ctatctatac gatatgtatg gagtaatgat ggtaatgatt ttattcttat
     5701 gtcaactagt gaagaaaata aggtgtcaca agttaaaata agattcgtta atgtttttaa
     5761 agataagact ttggcaaata agctatcttt taactttagt gataaacaag atgtacctgt
     5821 aagtgaaata atcttatcat ttacaccttc atattatgag gatggattga ttggctatga
     5881 tttgggtcta gtttctttat ataatgagaa attttatatt aataactttg gaatgatggt
     5941 atctggatta atatatatta atgattcatt atattatttt aaaccaccag taaataattt
     6001 gataactgga tttgtgactg taggcgatga taaatactac tttaatccaa ttaatggtgg
     6061 agctgcttca attggagaga caataattga tgacaaaaat tattatttca accaaagtgg
     6121 agtgttacaa acaggtgtat ttagtacaga agatggattt aaatattttg ccccagctaa
     6181 tacacttgat gaaaacctag aaggagaagc aattgatttt actggaaaat taattattga
     6241 cgaaaatatt tattattttg atgataatta tagaggagct gtagaatgga aagaattaga
     6301 tggtgaaatg cactatttta gcccagaaac aggtaaagct tttaaaggtc taaatcaaat
     6361 aggtgattat aaatactatt tcaattctga tggagttatg caaaaaggat ttgttagtat
     6421 aaatgataat aaacactatt ttgatgattc tggtgttatg aaagtaggtt acactgaaat
     6481 agatggcaag catttctact ttgctgaaaa cggagaaatg caaataggag tatttaatac
     6541 agaagatgga tttaaatatt ttgctcatca taatgaagat ttaggaaatg aagaaggtga
     6601 agaaatctca tattctggta tattaaattt caataataaa atttactatt ttgatgattc
     6661 atttacagct gtagttggat ggaaagattt agaggatggt tcaaagtatt attttgatga
     6721 agatacagca gaagcatata taggtttgtc attaataaat gatggtcaat attattttaa
     6781 tgatgatgga attatgcaag ttggatttgt cactataaat gataaagtct tctacttctc
     6841 tgactctgga attatagaat ctggagtaca aaacatagat gacaattatt tctatataga
     6901 tgataatggt atagttcaaa ttggtgtatt tgatacttca gatggatata aatattttgc
     6961 acctgctaat actgtaaatg ataatattta cggacaagca gttgaatata gtggtttagt
     7021 tagagttggg gaagatgtat attattttgg agaaacatat acaattgaga ctggatggat
     7081 atatgatatg gaaaatgaaa gtgataaata ttatttcaat ccagaaacta aaaaagcatg
     7141 caaaggtatt aatttaattg atgatataaa atattatttt gatgagaagg gcataatgag
     7201 aacgggtctt atatcatttg aaaataataa ttattacttt aatgagaatg gtgaaatgca
     7261 atttggttat ataaatatag aagataagat gttctatttt ggtgaagatg gtgtcatgca
     7321 gattggagta tttaatacac cagatggatt taaatacttt gcacatcaaa atactttgga
     7381 tgagaatttt gagggagaat caataaacta tactggttgg ttagatttag atgaaaagag
     7441 atattatttt acagatgaat atattgcagc aactggttca gttattattg atggtgagga
     7501 gtattatttt gatcctgata cagctcaatt agtgattagt gaatagataa aaatatgtta
     7561 aatatatcct cttatactta aatatataaa aataaacaaa atgatacact acataaagtg
     7621 ttctatctaa tatgaagatt taccaataaa aaggtggact atgatgaatg cacagtagtt
     7681 caccttttta tatttctaat ggtaacaaaa tattttttta tataaaccta ggaggcgtta
     7741 tgaatatgac aatatctttt ttatcagagc atatatttat aaagttagta attttaacta
     7801 tatcatttga tacattatta ggatgtttaa gtgcaataaa aagtcgtaaa tttaattcta
     7861 gttttggaat agatggagga atcagaaaag tagcaatgat agcatgtata ttttttttat
     7921 cagtagttga cattcttaca aagtttaact ttttatttat gttaccacaa gattgtatca
     7981 attttttaag actaaaacat cttggaatat ctgaattttt ctctatttta tttattttat
     8041 atgaaagtgt aagtatatta aaaaatatgt gcttatgtgg attaccagta cctaagagat
     8101 taaaggaaaa aatagcaatt ttactagatg caatgacaga tgaaatgaat gctaaggatg
     8161 aaaagtaagt aatggtagat ataataaaga tattaacaaa taaaaagtgt tatccaaata
     8221 agaatagctg aaagttatca taattcatga aactaataat gaaaacgagg gagcagatgc
     8281 caagagacac acaagtatta aatacatata atttcgaagc aagtgttcat tactatatag
     8341 atgacaaggt agtatatcaa acattggttc acaaagatgg tgcatggtca gttggtaaaa
     8401 tctattaagc tacattagtt acagatatca caaactataa tagttaaaca tagaaatatg
     8461 tgtaaattgt gatggaaatt attcaaaaac acaaaaatac gtgatgaagg acaaaatgat
     8521 atagaaaata agtatcaaac cttaataaat gatttaattg atagtttaaa agttatagga
     8581 aaaatatata aagaaataaa aacattaaaa aaatataaga tatgtttaca aattactatc
     8641 agacaatctc cttatctaat agaagagtca attaactaat tgagtatctt taaattgaaa
     8701 tgttaggaag tgatttaaat atgaaaactt aaattataaa aaatcaatat taatttattt
     8761 ttaaaaaata gaaaggagtg tataagattt attttcaaag tttaaaaaca agaaaatcaa
     8821 tttaaatttc agaaggaata aatgtggtta tagaagtgga tttattatca aaaataataa
     8881 tactaggagg tttttatgtc tttaatatct aaagaagagt taataaaact cgcatatagc
     8941 attagaccaa gagaaaatga gtataaaact atactaacta atttagacga atataataag
     9001 ttaactacaa acaataatga aaataaatat ttacaattaa aaaaactaaa tgaatcaatt
     9061 gatgttttta tgaataaata taaaacttca agcagaaata gagcactctc taatctaaaa
     9121 aaagatatat taaaagaagt aattcttatt aaaaattcca atacaagccc tgtagaaaaa
     9181 aatttacatt ttgtatggat aggtggagaa gtcagtgata ttgctcttga atacataaaa
     9241 caatgggctg atattaatgc agaatataat attaaactgt ggtatgatag tgaagcattc
     9301 ttagtaaata cactaaaaaa ggctatagtt gaatcttcta ccactgaagc attacagcta
     9361 ctagaggaag agattcaaaa tcctcaattt gataatatga aattttacaa aaaaaggatg
     9421 gaatttatat atgatagaca aaaaaggttt ataaattatt ataaatctca aatcaataaa
     9481 cctacagtac ctacaataga tgatattata aagtctcatc tagtatctga atataataga
     9541 gatgaaactg tattagaatc atatagaaca aattctttga gaaaaataaa tagtaatcat
     9601 ggtatagata tcagggctaa tagtttgttt acagaacaag agttattaaa tatttatagt
     9661 caggagttgt taaatcgtgg aaatttagct gcagcatctg acatagtaag attattagcc
     9721 ctaaaaaatt ttggcggagt atatttagat gttgatatgc ttccaggtat tcactctgat
     9781 ttatttaaaa caatatctag acctagctct attggactag accgttggga aatgataaaa
     9841 ttagaggcta ttatgaagta taaaaaatat ataaataatt atacatcaga aaactttgat
     9901 aaacttgatc aacaattaaa agataatttt aaactcatta tagaaagtaa aagtgaaaaa
     9961 tctgagatat tttctaaatt agaaaattta aatgtatctg atcttgaaat taaaatagct
    10021 ttcgctttag gcagtgttat aaatcaagcc ttgatatcaa aacaaggttc atatcttact
    10081 aacctagtaa tagaacaagt aaaaaataga tatcaatttt taaaccaaca ccttaaccca
    10141 gccatagagt ctgataataa cttcacagat actactaaaa tttttcatga ttcattattt
    10201 aattcagcta ccgcagaaaa ctctatgttt ttaacaaaaa tagcaccata cttacaagta
    10261 ggttttatgc cagaagctcg ctccacaata agtttaagtg gtccaggagc ttatgcgtca
    10321 gcttactatg atttcataaa tttacaagaa aatactatag aaaaaacttt aaaagcatca
    10381 gatttaatag aatttaaatt cccagaaaat aatctatctc aattgacaga acaagaaata
    10441 aatagtctat ggagctttga tcaagcaagt gcaaaatatc aatttgagaa atatgtaaga
    10501 gattatactg gtggatctct ttctgaagac aatggggtag actttaataa aaatactgcc
    10561 ctcgacaaaa actatttatt aaataataaa attccatcaa acaatgtaga agaagctgga
    10621 agtaaaaatt atgttcatta tatcatacag ttacaaggag atgatataag ttatgaagca
    10681 acatgcaatt tattttctaa aaatcctaaa aatagtatta ttatacaacg aaatatgaat
    10741 gaaagtgcaa aaagctactt tttaagtgat gatggagaat ctattttaga attaaataaa
    10801 tataggatac ctgaaagatt aaaaaataag gaaaaagtaa aagtaacctt tattggacat
    10861 ggtaaagatg aattcaacac aagcgaattt gctagattaa gtgtagattc actttccaat
    10921 gagataagtt catttttaga taccataaaa ttagatatat cacctaaaaa tgtagaagta
    10981 aacttacttg gatgtaatat gtttagttat gattttaatg ttgaagaaac ttatcctggg
    11041 aagttgctat taagtattat ggacaaaatt acttccactt tacctgatgt aaataaaaat
    11101 tctattacta taggagcaaa tcaatatgaa gtaagaatta atagtgaggg aagaaaagaa
    11161 cttctggctc actcaggtaa atggataaat aaagaagaag ctattatgag cgatttatct
    11221 agtaaagaat acattttttt tgattctata gataataagc taaaagcaaa gtccaagaat
    11281 attccaggat tagcatcaat atcagaagat ataaaaacat tattacttga tgcaagtgtt
    11341 agtcctgata caaaatttat tttaaataat cttaagctta atattgaatc ttctattggt
    11401 gattacattt attatgaaaa attagagcct gttaaaaata taattcacaa ttctatagat
    11461 gatttaatag atgagttcaa tctacttgaa aatgtatctg atgaattata tgaattaaaa
    11521 aaattaaata atctagatga gaagtattta atatcttttg aagatatctc aaaaaataat
    11581 tcaacttact ctgtaagatt tattaacaaa agtaatggtg agtcagttta tgtagaaaca
    11641 gaaaaagaaa ttttttcaaa atatagcgaa catattacaa aagaaataag tactataaag
    11701 aatagtataa ttacagatgt taatggtaat ttattggata atatacagtt agatcatact
    11761 tctcaagtta atacattaaa cgcagcattc tttattcaat cattaataga ttatagtagc
    11821 aataaagatg tactgaatga tttaagtacc tcagttaagg ttcaacttta tgctcaacta
    11881 tttagtacag gtttaaatac tatatatgac tctatccaat tagtaaattt aatatcaaat
    11941 gcagtaaatg atactataaa tgtactacct acaataacag aggggatacc tattgtatct
    12001 actatattag acggaataaa cttaggtgca gcaattaagg aattactaga cgaacatgac
    12061 ccattactaa aaaaagaatt agaagctaag gtgggtgttt tagcaataaa tatgtcatta
    12121 tctatagctg caactgtagc ttcaattgtt ggaataggtg ctgaagttac tattttctta
    12181 ttacctatag ctggtatatc tgcaggaata ccttcattag ttaataatga attaatattg
    12241 catgataagg caacttcagt ggtaaactat tttaatcatt tgtctgaatc taaaaaatat
    12301 ggccctctta aaacagaaga tgataaaatt ttagttccta ttgatgattt agtaatatca
    12361 gaaatagatt ttaataataa ttcgataaaa ctaggaacat gtaatatatt agcaatggag
    12421 gggggatcag gacacacagt gactggtaat atagatcact ttttctcatc tccatctata
    12481 agttctcata ttccttcatt atcaatttat tctgcaatag gtatagaaac agaaaatcta
    12541 gatttttcaa aaaaaataat gatgttacct aatgctcctt caagagtgtt ttggtgggaa
    12601 actggagcag ttccaggttt aagatcattg gaaaatgacg gaactagatt acttgattca
    12661 ataagagatt tatacccagg taaattttac tggagattct atgctttttt cgattatgca
    12721 ataactacat taaaaccagt ttatgaagac actaatatta aaattaaact agataaagat
    12781 actagaaact tcataatgcc aactataact actaacgaaa ttagaaacaa attatcttat
    12841 tcatttgatg gagcaggagg aacttactct ttattattat cttcatatcc aatatcaacg
    12901 aatataaatt tatctaaaga tgatttatgg atatttaata ttgataatga agtaagagaa
    12961 atatctatag aaaatggtac tattaaaaaa ggaaagttaa taaaagatgt tttaagtaaa
    13021 attgatataa ataaaaataa acttattata ggcaatcaaa caatagattt ttcaggcgat
    13081 atagataata aagatagata tatattcttg acttgtgagt tagatgataa aattagttta
    13141 ataatagaaa taaatcttgt tgcaaaatct tatagtttgt tattgtctgg ggataaaaat
    13201 tatttgatat ccaatttatc taatactatt gagaaaatca atactttagg cctagatagt
    13261 aaaaatatag cgtacaatta cactgatgaa tctaataata aatattttgg agctatatct
    13321 aaaacaagtc aaaaaagcat aatacattat aaaaaagaca gtaaaaatat attagaattt
    13381 tataatgaca gtacattaga atttaacagt aaagatttta ttgctgaaga tataaatgta
    13441 tttatgaaag atgatattaa tactataaca ggaaaatact atgttgataa taatactgat
    13501 aaaagtatag atttctctat ttctttagtt agtaaaaatc aagtaaaagt aaatggatta
    13561 tatttaaatg aatccgtata ctcatcttac cttgattttg tgaaaaattc agatggacac
    13621 cataatactt ctaattttat gaatttattt ttggacaata taagtttctg gaaattgttt
    13681 gggtttgaaa atataaattt tgtaatcgat aaatacttta cccttgttgg taaaactaat
    13741 cttggatatg tagaatttat ttgtgacaat aataaaaata tagatatata ttttggtgaa
    13801 tggaaaacat cgtcatctaa aagcactata tttagcggaa atggtagaaa tgttgtagta
    13861 gagcctatat ataatcctga tacgggtgaa gatatatcta cttcactaga tttttcctat
    13921 gaacctctct atggaataga tagatatatc aataaagtat tgatagcacc tgatttatat
    13981 acaagtttaa taaatattaa taccaattat tattcaaatg agtactaccc tgagattata
    14041 gttcttaacc caaatacatt ccacaaaaaa gtaaatataa atttagatag ttcttctttt
    14101 gagtataaat ggtctacaga aggaagtgac tttattt
//
LOCUS       DAEPIN010000086        14105 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.086, whole genome shotgun sequence.
ACCESSION   DAEPIN010000086 DAEPIN010000000
VERSION     DAEPIN010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14105
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.086"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            458..871
                     /locus_tag="KR250_003170"
     CDS             458..871
                     /locus_tag="KR250_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBH0069064.1"
                     /translation="MELEITEKIEKQDEDTIFQGLLKYNLARLEDKNPVDLGIYIRDK
                     AGKIVAGFIGVTHGNWLSIKYLWVSEKLRYKGTGSQLLYKAEKIAKERGCKYVFLDTF
                     SFQAPKFYEKFGYHKVFSLENYPLTGKRYYFRKIL"
     gene            1062..2279
                     /locus_tag="KR250_003171"
     CDS             1062..2279
                     /locus_tag="KR250_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HBH0069065.1"
                     /translation="MLGKKCMDYLQTLGKISSTTNGLTRLILTQEHKKSIDLISSWME
                     GLNLDIEIDDIGNVIGTYKSSFPNAPTLVVASHQDSVKCGGIFDGMLGIIVPLVGLEE
                     AKHNNRSYPFNIKLIAFAEEEGTRFETSLMGSKVFAGTFKEELLKSVDENGITLEEAV
                     TKFGFNTKNLTNLHPRKDVDAYLEFHIEQGPVLENESLPAGIVSSITGFKSFKISVNG
                     KSGHAGTLPMNMRLDAGCCACECVLAIEKVAKTTADLVATVGKMNFYPSSSNVVPERA
                     EFTLDVRSCSQEILDNSVEKIFNEISHICENRKLNYTSELAFENVPVPCSNKITKIIE
                     KSFIDLNLNPFYIYSGAGHDAQEMDNITDIGMVFIRCAGGVSHNPNESVSVDDLDTAV
                     KIFLKILDNLDLK"
     gene            2441..3139
                     /locus_tag="KR250_003172"
     CDS             2441..3139
                     /locus_tag="KR250_003172"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HBH0069066.1"
                     /translation="MNEKLIGILAGMGPRSTAPFVDLIIDECQSQYGAKYDDEFPKMM
                     IYSLPTPFYIDRPINHELMKETIIDGLQKLESTGVNFIAMPCNSAHIYFKELKESINI
                     PILNIVEETVKKLPTISQKVTLFSTSSTFESTIYQNGIIHNGHEFIFKDEWQIKLNNL
                     IQNIKMDKENPHNIDIWNKLIEDVKNESIENIVIACTDLNVVLEKSYPSINIVDSSKC
                     LAESVVNKYLKLVK"
     gene            complement(3359..3976)
                     /locus_tag="KR250_003173"
     CDS             complement(3359..3976)
                     /locus_tag="KR250_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4878 domain-containing protein"
                     /protein_id="HBH0069067.1"
                     /translation="MKKIILALTTFLLAISLTACSSAKPEDTIDSFFNSAKKFDFEGM
                     NKVMENNDEKYKDILKELDTKDPNAQYVLDYLKQNASKITYTIKDSEVKGDKATIKVE
                     CKFIDSTPLLQEIVAEAFTKMIGMSFSGQDLTDEKTTEMLVSIMKEKQKSVKETYVTK
                     NVEFECSKKDNKWIISSVNDAVADVLLSNLVTAGQEFSNSMNTLE"
     gene            complement(4103..5125)
                     /gene="argF"
                     /locus_tag="KR250_003174"
     CDS             complement(4103..5125)
                     /gene="argF"
                     /locus_tag="KR250_003174"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HBH0069068.1"
                     /translation="MNNISLKGKSFLTLKDFTKEEIRYLLTLSSYLKTKKKVGIKGDL
                     LQGKNIALLFEKTSTRTRCSFEVAAHDEGAHVTYLGPNDCHMGKKESVSDTAKVLGRF
                     YDGIEFRGFKQETVESLAKYSGVPVWNGLTDEYHPTQILADFLTVIENVDKDLNKVKF
                     VYVGDARNNMGNSLMIGCAKMGIHFVALAPKELWPNEELVKEMKEIAKKSKGDIMLTE
                     NIDDVKGADVIYTDVWVSMGEEEQYEARINQLKDYQVNMDMIKKTENENVIFLHCLPA
                     FHDLETKVAQEIYEKFGLSELEVTNEVFNSKYSKVFEEAENRMHTIKAVMVSTMTDVN
                     MNNNLI"
     gene            complement(5234..6421)
                     /locus_tag="KR250_003175"
     CDS             complement(5234..6421)
                     /locus_tag="KR250_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HBH0069069.1"
                     /translation="MNNDNTISKWNEYFIDTYNQPNFVIDYGEGSCFFDTNGNKYIDF
                     TSGYGVSSLGYSNSNLKNALKEQVDKLLHTSNLYFNEPVLYSGEKIINSSGMAKVYFC
                     NSGTEANETAFKIARKYSSDKYGNGRGTIISLKDSFHGRTMMSLMATGMDKYHKYFYP
                     LPEGFKYVERNNIEDLKNNLDSTVCAIILEAIQGEGGVNVLEKDYVLEIVKICQEKDI
                     VVIFDEVQCGIGRTGKLFGYEYFDVKPDIVTVAKGLGAGIPVGGVLVNKKLSKVLGKG
                     DQGTTFGGNLLAMVAASVVLDEISKDGFYNEVLEKGNYIRKSIESFNNKVVLKTKGIG
                     LMIGIETNIESSIIEEKARKKGLLILTAGKNVLRFLPPLTISYKEIDEALEILKDILL
                     EIN"
     gene            complement(6451..7311)
                     /gene="argB"
                     /locus_tag="KR250_003176"
     CDS             complement(6451..7311)
                     /gene="argB"
                     /locus_tag="KR250_003176"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HBH0069070.1"
                     /translation="MINIEKANTLIEALPYIEKHQGKTIVVKYGGSAMKKDGLKESVM
                     EDLVLMSYVGINIVLVHGGGAEINKMLAKVDIESKFVNGLRYTDEETMEIVKMVLAGK
                     VNKDLVNKIHTKGGKAVGLCGIDNNMILCDPYKNYELGFVGEIKKVNVELIESCLKSG
                     YISVIATIGVGDDGETYNINGDTAASAIAKELNADKLILLTDVPGLLREPDEEKSLIT
                     EVILEDVDKLFEEGIITGGMIPKIEGCVDALNNGVNRVHILDGRVPHSIITELFTDSG
                     IGTLIRKENE"
     gene            complement(7326..8543)
                     /gene="argJ"
                     /locus_tag="KR250_003177"
     CDS             complement(7326..8543)
                     /gene="argJ"
                     /locus_tag="KR250_003177"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HBH0069071.1"
                     /translation="MEIIKGGVTVSEGFFASGIHCGLRKNKEKRDLALVYSDVLCDAA
                     AVVTQNKVKGNPVYVTQEHIKNGKAQAIIVNSANANTFNGKEGLINAYKMAKFTSDKL
                     NIKESDVLVASTGVIGKPLNIDLIEENMDELVNNLSKQGHIGAREAIMTTDIIKKEIA
                     VAIMYGDKKITIGGMAKGSGMIHPNMATTLGFITTDANIDGVLLKEALKIAVDKSFNR
                     VSVDGDTSTNDMVMILANGKAKNDRINKKDEHYQVFLSALTYVCIELAKLVAKDGEGC
                     TKLIECYINGALSEAHAVKLAKTVISSSLVKTAVFGADANWGRILCALGYAGEEIDME
                     KVDIIFESMKGYIEVCKNGNGLDFNEEKAKKILEDDEISILVDLNMGNARGNAWGCDL
                     SYDYVRINGSYRN"
     gene            complement(8584..9618)
                     /locus_tag="KR250_003178"
     CDS             complement(8584..9618)
                     /locus_tag="KR250_003178"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HBH0069072.1"
                     /translation="MNKIKVGIIGATGYVGAELIRLLMNHDKVEVTAIGSNSYVGKDI
                     VDIYPSIGYKNNMICIENEKVIDMCDVVFTALPHGVSEKFVIKAIKSKKKVIDLGADF
                     RIKDEEVYSKWYGVSFIDKILHKKAVYGLSEIYKEDIKDADIIANPGCYPTSISLPLM
                     PLLSSKLIKNNNIIIDSKSGLTGAGRELSISSHFTEVNENITAYKIGKHRHTPEIEQN
                     LSESCKEKVSVVFTPNLIPVNRGILSTIYCTKEDNISINDIHRKLTDYYEFKEFIEIL
                     PLDKIASLKNVRFSNKCVISLHENGDTLIICSAIDNMIKGAAGQAIQNMNIIFGIEEN
                     TGLKNIAPSF"
     misc_binding    complement(9872..10085)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(10303..11070)
                     /locus_tag="KR250_003179"
     CDS             complement(10303..11070)
                     /locus_tag="KR250_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaP domain-containing protein"
                     /protein_id="HBH0069073.1"
                     /translation="MQIIDININNIDNEHICCSISEKKGENCVFSKKAWMKNNFDKGL
                     VFKRFDVRGKAFIEYIPAENAWCPITADNYIFINCFWISGKFKGQGYSNLLLQECVDD
                     AKSKGKYGLVVLSSSKKMPFLSDAKYLKYKDFCLADTSEPNYELLYFPFTDNAPIPCF
                     KNCVKQRKLNENGIVLYYSNQCPHTEKYAPILKKIANSRGIDVNLIKFETAKEAQNAP
                     APFTTYSLFFNGEFVTNEILSEKKIEKFLDTNIKKFN"
     gene            11369..11842
                     /locus_tag="KR250_003180"
     CDS             11369..11842
                     /locus_tag="KR250_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBH0069074.1"
                     /translation="MFKEYLIRELKEDDIKNALDLIWNVFEEFESPDYCDEGITTFHQ
                     FIEYNSIIEKIKEKTLFFWGCFDSKKLIGVLAIRNLNHICMLFVDKNYHKHGVAKKLM
                     TTGIQYCICNKFNLTEITVNSSPYAVEVYHKLGFKDTAVEQIVDGIRFTPMKLLI"
     gene            complement(12211..12768)
                     /locus_tag="KR250_003181"
     CDS             complement(12211..12768)
                     /locus_tag="KR250_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBH0069075.1"
                     /translation="MRVSREIVIQATSDIVNKDGLNKVSLKVIAEKLGIRTPSLYNHI
                     ESLDDLLLEVAHKGMREMNSQMIKAAIGNFGDTAIKCISIAYFNFIISNPGVYETIQW
                     ATWHGNNETLEIFDEYKSLLEKLILSCNLNTKNTGEVLNLIMSVLHGYSTLQLGKSFL
                     SKEEAIKGLVDSIDTVLLGIHKKYD"
     gene            13026..13724
                     /locus_tag="KR250_003182"
     CDS             13026..13724
                     /locus_tag="KR250_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBH0069076.1"
                     /translation="MEKKYFEIHGVPSILWGNPSSKLYIYIHGQCGNKEEAEAFANIA
                     VPHECQVLSVDLPEHGERKREKNSFNPWHVVPELVSIMEYAKCHWNQISLYANSIGAW
                     FSMLSFEKENLRECIFVSPILDMQQLISNMMLWANVSEEQLKHELIIPTSFGHTLSWE
                     YLQYVKQHPITQWNIPTKILYGKHDELTEIGVVEKFVEKFNCNLTIVENGKHWFNTPQ
                     ELNSLYEWLNTLVK"
BASE COUNT         4844 a         2199 c         1532 g         5530 t
ORIGIN      
        1 aaataaaata ctatcgagtt tattttggta gtattttatt ttttaattat taatctctac
       61 taggtattta ttattttatt acttatattt aataagacta tttactactt tctaatctta
      121 tttatataat ttattcattt tataaattga actactaatg atattattga acatagcaat
      181 agaataacat ctaaaatagt ttttatgtat taaattaact tattttaata ttcaacaatt
      241 taggtttact aaatttattg atgatttctt tttatataac ttaaactaca tttttattta
      301 aaaaaattta atatcaacaa tataaaatta agaataagta ttattagttt gacattagca
      361 taaatgccta ttagaataat gttaaactat attctttcta ctgattttat taaaaaatac
      421 caatatacaa aagattatac aaggagaaag ttcaaatatg gaattagaaa ttacagaaaa
      481 aatagaaaaa caagatgaag atactatttt tcaaggatta ttaaaatata atcttgctag
      541 acttgaagat aaaaacccag tggatttagg aatatatatt cgagataaag caggtaaaat
      601 tgttgcaggt tttataggtg ttactcatgg taattggctt tctataaaat acttatgggt
      661 tagtgagaag cttagatata aaggtactgg aagtcaactt ttatataagg ctgaaaaaat
      721 tgctaaagaa cgaggttgta aatatgtttt tttagataca ttcagttttc aagcacctaa
      781 attttatgag aaatttggat accataaagt attttcactt gaaaattatc ctctaactgg
      841 aaagagatat tattttagaa aaatactata ataataaaaa tcatatttta tatttttaac
      901 ttaaaacttc tgctcttcgt ctacagactt tatattttta tatcattatt aatactaaaa
      961 tagtatttta ctatacatac aaaattattt gaaagttcaa aaattataag tataaaagct
     1021 aatcttagct taaataaaaa ttatattttt aggaggtaaa tatgttagga aaaaaatgta
     1081 tggattatct acaaacatta ggaaaaataa gttcaacaac aaatggtctt acaagactta
     1141 tcttgactca agaacataaa aaatctattg atttaattag ttcatggatg gaaggtttaa
     1201 atcttgatat tgaaattgat gacattggaa acgttatagg tacatataaa tcaagctttc
     1261 caaatgctcc cacattagta gttgcatctc atcaagatag tgtaaagtgt ggaggtatat
     1321 ttgatggtat gttaggtata atagtgcctc tagttggtct tgaagaagca aaacacaata
     1381 ataggtctta tcctttcaat attaagctaa ttgctttcgc tgaagaggaa ggaactagat
     1441 ttgaaacttc tttaatggga agtaaagttt ttgctggtac ttttaaagag gaacttctta
     1501 aatctgttga tgaaaatgga attacacttg aagaagctgt aacaaagttt ggatttaata
     1561 caaaaaactt aacaaatttg catcctagaa aagatgtaga tgcatattta gaatttcata
     1621 ttgagcaggg tcctgtttta gaaaatgaat ctcttcctgc tggaattgta tcaagtatta
     1681 caggatttaa aagttttaag atatcagtaa atggtaagtc tggacatgct ggtacacttc
     1741 caatgaatat gagacttgat gctggatgtt gtgcctgtga atgtgtatta gctatcgaaa
     1801 aagtagctaa aacgactgct gatttggttg ctactgtagg aaagatgaat ttctatccat
     1861 cttcatcaaa tgtggtacct gaaagagctg aatttactct tgatgtacgc tcttgttcac
     1921 aagaaattct tgataattct gtagaaaaga tatttaatga aatatcacat atatgtgaaa
     1981 atagaaaact taattataca agtgaacttg cttttgaaaa tgttcctgta ccatgttcta
     2041 ataaaataac aaaaatcata gaaaaaagtt ttatagattt aaatcttaat cctttttata
     2101 tttatagtgg tgctggccat gatgctcagg aaatggataa tataactgat attggtatgg
     2161 tatttataag atgcgctggg ggcgtaagtc ataatcctaa tgaaagtgtt tctgtagatg
     2221 acttggatac tgctgtaaaa atatttttaa aaatactaga taatcttgat ttaaaataat
     2281 tcttatataa gaaagtatat taaaatatat aatctaaaaa atcactttat atttatatta
     2341 tataagtgta caatataatc tattataaaa tataatttta tgcttttata gatttttatt
     2401 ttatacataa atatatttat atataaagga gataattttt atgaatgaaa aattaattgg
     2461 tattttggct ggaatgggtc caaggtcaac agcccctttt gttgatttaa ttattgatga
     2521 gtgtcaatct caatatggag caaaatatga tgatgaattt ccaaagatga tgatatattc
     2581 attacctacc ccattttata ttgaccgtcc aataaatcat gaacttatga aagaaactat
     2641 aattgatgga ttacaaaagc ttgaatcaac tggtgtaaat tttattgcaa tgccatgcaa
     2701 ttcagcacat atatacttta aagaattaaa agagtctatt aatataccaa tattaaatat
     2761 tgtagaagaa acagttaaaa aattacctac tatctcacaa aaagtaactc ttttttcgac
     2821 tagttcaact tttgagtcta caatttacca gaatggtata atacataatg gacatgaatt
     2881 tatatttaaa gatgaatggc aaataaaatt aaacaatctt attcaaaata taaaaatgga
     2941 taaagaaaat ccacacaata ttgatatttg gaataaactt atcgaagatg ttaaaaatga
     3001 atctatagaa aatatagtaa ttgcttgtac tgatttaaat gtagttttag aaaaatcata
     3061 tccttcaata aatattgttg attcatcaaa atgcttggct gaatcagttg tcaataaata
     3121 tctaaaatta gttaaataaa tttttcatgc actatattat aaaaaatata tgttataagt
     3181 ttattttaaa tagtatagac ttataacata tatcatagtt tcttgacaat atagaaatta
     3241 accatgtatt tcattatatt aataaaaagg ataccaaaaa tggtatcctt cataaatatt
     3301 tatatgttta tttataaaaa taatcatata tttattactt tactacacct aaacaatatt
     3361 attcaagtgt attcatagag ttagaaaatt cttgtcctgc tgtaacaaga ttagataaca
     3421 atacatcagc tactgcatca tttactgaac ttattatcca tttattatcc tttttagaac
     3481 attcaaactc tacatttttt gtaacataag tctctttaac gcttttttgt ttttctttca
     3541 taattgatac taacatttca gttgttttct catcagttaa gtcttgtcct gaaaaactca
     3601 taccaatcat ctttgtaaat gcctcagcta ctatttcttg aagtaatggt gttgaatcta
     3661 taaatttaca ttctactttg attgtagcct tatcaccttt aacctcgctg tcttttattg
     3721 tatatgtgat tttagatgca ttctgtttta gataatctaa aacatattgg gcatttgggt
     3781 cttttgtatc taattccttt aatatatcct tatatttttc gtcattattt tccattacct
     3841 tattcatgcc ttcaaagtca aactttttcg cactgttaaa aaaactatca attgtatctt
     3901 ctggttttgc acttgaacat gctgttaaag atattgctaa taaaaaagtt gttaaagcta
     3961 atatgatttt tttcatatct caaatcctcc ctcttaattt gttgatacat aagttatttt
     4021 aacatataat gagagttttt gaataatata tttaaaatat tttataaatt ttcattaaag
     4081 taaagtctat attaaaacat atctatatta aattattgtt catatttaca tcagtcatag
     4141 tagaaaccat aacagctttt attgtatgca ttctattttc agcttcttca aatacctttg
     4201 aatattttga attaaatacc tcgtttgtaa cctctaattc tgataatccg aacttttcat
     4261 aaatctcctg agcaacctta gtttctaaat catgaaatgc tggaagacag tgtaaaaata
     4321 taacattttc attttcagtt ttctttatca tatccatatt tacttgataa tctttaagtt
     4381 gatttattct tgcttcatat tgctcctctt ctcccataga aacccataca tctgtatata
     4441 taacatctgc acctttaaca tcatcaatat tttcagtcaa cattatatct cctttggatt
     4501 tcttggctat ttccttcatt tccttaacca attcttcatt tggccaaagt tccttaggtg
     4561 ctagtgctac gaaatgtatt cccattttag cacatcctat catcagtgag tttcccatat
     4621 tatttctagc atcaccaaca tatacaaatt taactttatt caaatcttta tctacatttt
     4681 caattactgt taaaaaatct gccagtattt gtgttggatg atattcatct gtaagtccat
     4741 tccaaacagg cacaccagaa tattttgcta gactttcaac tgtttcttgt ttaaagcctc
     4801 taaattctat tccatcatag aatcttccta gaacttttgc tgtatctgat acagactctt
     4861 ttttacccat atgacaatca tttggaccta gataagttac atgtgctcct tcatcatgag
     4921 ctgctacttc aaatgaacat ctagttcttg ttgatgtttt ctcaaataat aaagctatat
     4981 tttttccttg tagcaaatcg ccttttatac caactttttt cttagttttt aaataacttg
     5041 ataaagttaa taaatatcta atctcttctt ttgtaaaatc ctttaatgtc aaaaaactct
     5101 taccttttaa acttatatta ttcatattaa ttcgcccctt cttataaact ttttaaattc
     5161 aattaaactt gttagcactt tactttttta tttacttcta atttttatct ttaatcaact
     5221 atttcttgaa tatttagtta atttctagta atatatcttt taggatttca agagcctcat
     5281 ctatttcctt ataactgatt gttaatggtg gtaaaaatct caatacattt tttccagcag
     5341 tcaaaataag caatcctttt tttctagctt tctcttctat aatactagat tcaatattag
     5401 tttcaattcc tatcattaga ccaatacctt ttgtctttaa tacaactttg ttattaaaac
     5461 tttcaataga ttttcttata taatttcctt tttctaatac ttcattataa aaaccatctt
     5521 tagatatctc atctaataca actgaagctg ccaccatagc taataaatta cctccaaaag
     5581 ttgtaccttg gtctcctttt cctagtactt ttgaaagttt tttatttact aatactcctc
     5641 ctactggtat tcctgctccc aacccttttg ctacggtaac tatatctggt tttacatcaa
     5701 aatattcata gccaaaaagt ttaccagttc ttcctatacc gcattggact tcatcaaaaa
     5761 tcactacaat atccttttct tgacatattt ttactatttc caaaacataa tctttttcaa
     5821 gaacattaac tccaccttcg ccttgaatcg cttcaagaat tattgcacaa actgtggaat
     5881 caagattatt ttttaaatct tcaatattat ttctttctac atatttaaaa ccttcgggaa
     5941 gaggataaaa atacttatgg tatttgtcca taccagttgc cattaaagac atcattgttc
     6001 taccatgaaa agagtctttt aaacttatga tagttcctct accattacca tatttgtcac
     6061 tactatactt tcttgctatt ttgaatgctg tttcatttgc ttctgttcca gaattacaaa
     6121 aatatacttt agccattcca cttgaattaa ttattttctc tcctgaatac aacactggct
     6181 cattaaaata taaatttgaa gtatgtaata atttatctac ttgctctttt agagcattct
     6241 ttaaattact attactatac ccaagagaag atactccata tccacttgta aaatctatat
     6301 atttattgcc attagtatca aaaaaacaac ttccttcacc ataatctatg acaaaattgg
     6361 gttgattata tgtatctata aaatattcat tccattttga aattgtattg tcgttattca
     6421 ttcttattct cccctcttaa acttataatt ttattcattt tcttttctta ttaatgtacc
     6481 tattccacta tcagtaaata gctctgttat tatagaatgt ggaactcttc catccaaaat
     6541 atgtacccta tttactccgt tatttaaggc atctacgcat ccctctattt taggtatcat
     6601 acctccagta attatacctt cttcaaataa tttatcaaca tcctctaata taacttctgt
     6661 tattaatgat ttttcttcat ctggctctct taacaaacca gggacatctg taagaagtat
     6721 caatttatct gcatttaact ctttagctat agcagaagct gctgtgtcac catttatatt
     6781 ataagtctct ccatcatctc caacacctat agttgcaatt actgaaatat aacctgactt
     6841 tagacaagat tctataagct caacatttac ttttttgatt tctcctacaa atccaagttc
     6901 ataattttta tatgggtcac ataaaatcat attattatct attccacaaa gccctactgc
     6961 ttttccacct tttgtatgta ttttatttac taggtcttta ttaacctttc cagcaagtac
     7021 catttttaca atttccatgg tctcttcatc tgtatatcta agaccattta caaatttact
     7081 ttctatatcc acttttgcta gcatcttatt gatttctgcc cctccaccat gtactaaaac
     7141 tatattgatt ccaacataac tcattaacac aaggtcttcc ataacagatt cttttagacc
     7201 atcttttttc attgcacttc caccatactt aactacaata gtctttccct gatgtttttc
     7261 tatatatggt aaagcttcta taagtgtatt tgctttttct atattaatca ttaaagtata
     7321 tcctcctaat ttctataact tccatttatt cttacataat catagcttaa atcacatccc
     7381 catgcatttc ctctggcatt gcccatattt aaatctacta atatacttat ttcgtcatct
     7441 tcaaggattt tttttgcttt ttcctcatta aaatctaatc catttccatt tttacatact
     7501 tcaatatatc ctttcatact ctcaaaaata atatcaactt tttccatatc tatttcttca
     7561 cctgcataac ctaaagcaca cagtattcta ccccaatttg cgtcagctcc aaatacagct
     7621 gtctttacta atgaagaaga tataactgtt tttgctaatt ttactgcatg tgcttcactt
     7681 aatgctccat ttatatagca ttcaattagt tttgtacatc cttctccatc ttttgcaaca
     7741 agtttagcaa gttctataca gacatatgta agtgcactca aaaatacttg ataatgttca
     7801 tcttttttgt ttattctatc attttttgct tttccatttg ctagaatcat taccatatca
     7861 ttagtacttg tatctccatc tacactaact ctattaaaac ttttgtcaac agcaattttt
     7921 aaagcttctt ttagcaaaac tccatcaata tttgcatctg tagttataaa tcctaacgtt
     7981 gttgccatat taggatgtat cataccagaa ccttttgcca tacctccaat agttattttt
     8041 ttatctccat acattattgc tactgctatt tcttttttta taatatcagt tgtcataata
     8101 gcttctcttg ctccaatatg accttgttta cttagattat tcactaattc atccatattt
     8161 tcttctatta aatctatatt taatggcttt cctataacac ctgtagatgc aaccaaaaca
     8221 tcactttctt taatatttaa tttatcactg gtgaatttag ccattttata agcatttata
     8281 agtccttctt ttccattaaa ggtattagca ttagcactat ttactatgat agcttgtgct
     8341 tttccatttt taatgtgttc ctgtgtaaca taaacaggat tgccctttac tttattttga
     8401 gttactactg ctgctgcatc acataaaaca tctgaataaa ccaaagctaa atctcttttt
     8461 tctttatttt ttctcaagcc acagtgaatc cctgaagcaa aaaatccttc tgaaactgta
     8521 actccaccct ttataatctc catatttatc ctcctaatta tatgagttta gtatttttta
     8581 tttctaaaat gatggtgcaa tattttttaa acctgtattt tcttcaatac caaatattat
     8641 attcatgttt tgtatagctt gacctgctgc tcccttaatc atattatcta tagctgaaca
     8701 gattatcaat gtatctccat tttcatgtaa tgatattaca catttatttg agaatctaac
     8761 attctttaaa gaagctatct tatctaatgg aagaatttct ataaattctt taaattcata
     8821 ataatcagtt aatttcctat gtatatcatt tatagaaata ttatcttctt ttgtacaata
     8881 tatagttgat aagattcccc tatttactgg gattaagttt ggtgtaaaaa ctacacttac
     8941 tttttctttg caggattcac ttaaattttg ttctatttct ggagtatgtc tgtgtttccc
     9001 tattttatat gcagtgatat tttcattaac ctccgtaaag tgactactaa tcgataattc
     9061 tctaccagca cctgtaagac ctgatttaga atcaattatt atattattat tttttatcaa
     9121 tttagaactt agtaatggca tcaatggtaa agaaatacta gttggataac atccaggatt
     9181 tgcaattata tctgcatctt ttatatcttc tttgtatatt tcacttaatc catatacagc
     9241 ttttttatgt aagattttat ctataaaaga tactccatac catttactat atacttcttc
     9301 atcttttatt ctaaagtcag cacctaaatc tataactttc tttttcgatt taattgcctt
     9361 tattacaaac ttttcactaa ctccatgtgg aagggctgta aacacaacat cacacatatc
     9421 tataactttt tcattttcta tacatatcat gttattttta tatcctatac ttggatatat
     9481 atcaactata tcttttccaa catacgaatt tgaacctatc gctgtcactt cgactttatc
     9541 atgattcatt aataatctaa tcaactctgc cccgacatat ccagttgcac caataatacc
     9601 aacttttatt ttattcattt tgttccctcc atttttatat tcaaatagtc ttaaaatcct
     9661 atctttccac tttactatat gataatcatt tgataatacg tagattaaaa tactctaatt
     9721 aaaacaaaaa accccttagc attataaata ctaagaggcg attttccgcg gtaccactct
     9781 tattggtaaa attacaataa tcattaaaac tcaaattaaa aagtatctac aaaatgtaca
     9841 tttaaaatta aaaaagcctc ttggtcaaag accaagagac gaatatacac ccgcgttacc
     9901 actctaattg ataagataat cttacccact ctaatctctt aaggcgagat tcaccaatta
     9961 ccatactatt acttctggta atctcctcca aagctctatt cacaataaaa ttcgttacta
    10021 ggcttccacc attcccagct ctctatcaac tagctttatg ctactctctt tttcacagga
    10081 tttcaattta ttttcttatt gttttatatg atagtttatt tttttaactt tgtcaatata
    10141 atttttaaat ttttggaaat attttctata tggttaattt ccagcatttt gtatctaaat
    10201 tgcgatataa tttttatatt tcattcgtca atttaaaaaa catccaaaat atattaaatt
    10261 ataaaataat atattttcaa aatacaatat ttacatctct ttttaattaa attttttaat
    10321 gttggtatct aaaaatttct caatcttttt ctcagaaagt atctcgttgg ttacaaattc
    10381 accattaaaa aaaagactgt atgtagtaaa tggtgcagga gcattttgtg cttcttttgc
    10441 tgtttcgaat tttataagat ttacatcaat acctctagaa tttgcaattt ttttcaaaat
    10501 aggagcatat ttttccgtat gtggacactg attactgtaa tataaaacta taccattctc
    10561 atttaatttt ctttgtttta cacaattctt aaaacatgga ataggagcat tatcagtaaa
    10621 gggaaaatat aatagctcat aatttggttc tgatgtatct gctaaacaaa agtccttata
    10681 ttttagatat ttagcatcag ataaaaaagg cattttttta ctagacgaaa gcacaactaa
    10741 tccatattta ccttttgatt ttgcatcatc aacacactct tgtagcaata gattagaata
    10801 tccttgaccc ttaaattttc cagatatcca aaaacagtta ataaatatat aattgtccgc
    10861 agtaatagga caccatgcat tttctgctgg aatatattct ataaaagctt tacctcttac
    10921 atcaaatcgt ttaaagacta gacctttatc aaaattattt ttcatccaag cttttttaga
    10981 aaaaacacaa ttttctcctt ttttttcaga aattgaacaa catatatgtt cattgtcaat
    11041 attatttata tttatatcaa taatctgcat acttatctcc ttttctaatt gcataataat
    11101 ttttaaatat attctatata tttttatctt aatattttga atacaacaac atcatgtcat
    11161 atatattttt attttacata atattatttt aagattatct gaattattaa attgtatata
    11221 taattaaata cattctatgg tctaactaca ctataatata aattatatat tatcataata
    11281 ttctaaattt ataaaacaaa ttgtattgaa ttgaatttat ttaagatata taattataat
    11341 taaaataata ataaaggtgt tgtttataat gttcaaagaa tacttaataa gagaattaaa
    11401 agaagatgat attaaaaatg cattagactt aatctggaac gtatttgaag aatttgaatc
    11461 tccagattat tgtgatgaag gaattacgac atttcatcaa tttatagaat ataactctat
    11521 tattgaaaaa atcaaagaaa aaactctctt cttttggggt tgttttgact ctaaaaaact
    11581 tattggagtc ttagctatta gaaatttaaa tcatatatgt atgttatttg tagataaaaa
    11641 ctatcataaa catggtgttg caaaaaaatt aatgactaca ggcatacaat actgtatttg
    11701 taataaattt aatttaactg aaataactgt aaattcatca ccttatgctg ttgaagttta
    11761 tcataaatta ggatttaaag atactgctgt tgagcaaatt gttgacggta tacgttttac
    11821 acctatgaaa ttgttaatat aataaattaa ataaaaagca aagtctctta aatattttat
    11881 agttactttg cttttaaacc aagatttaaa ataatactat cttttttgtt ttttaataat
    11941 atactttaaa atccttacta atatgaacta taatttattt tttgatatcc tttttgatta
    12001 acatataaat taattcaatt attaaacata aactatgttg tttctcttta tatctcttct
    12061 ttgtaaacaa tattgggcat ttgaaaataa atattcttaa aatatactgt tctcctgttg
    12121 attttccttt ggtacaaatg ataaaaattt aatttacatg ccccataatg aagtgttcaa
    12181 taaataagtt aaaataattt atttacctcc ttaatcatat tttttatgaa tacctaataa
    12241 cacagtatca atagaatcga ctaaaccttt tatagcttct tctttactca aaaatgattt
    12301 cccaagttgc aaagtcgaat aaccatgaag tacactcatt attaagttta atacttcacc
    12361 tgtattcttt gtatttagat tacaagaaag aataagtttt tctaataagg atttatattc
    12421 atcaaagatt tctaatgttt cattatttcc atgccaagta gcccactgaa ttgtttcata
    12481 aacaccagga ttagaaatta taaaattaaa gtatgctata cttatacact ttatagctgt
    12541 atctccaaaa tttccaatag ccgcctttat catttgacta ttcatctctc tcattccttt
    12601 atgagctact tcaagtaata aatcatctag actttctata tgattgtaca aagatggtgt
    12661 acgtattcct agcttttctg caataacttt taaagaaact ttgttaagcc catctttatt
    12721 aactatatct gatgtagctt gtataactat ttctctacta acacgcatga ttttccctct
    12781 ttcatttaaa attaattact ttattagttt aactaatcta attagtttcg tcaattattg
    12841 taatttataa aattctttat actattataa aatcttgaca gtttaattat aatttaatat
    12901 aatactaatt aaattagttt taatactaat ataattagta ttatattaaa ttctatatta
    12961 caacattatt ttatagtttg attaatttaa taatcactaa tatatcaaac tgaaaggaga
    13021 tttttatgga aaaaaaatat ttcgaaattc atggagttcc ttcaatcctt tggggaaatc
    13081 cttcaagtaa attatatatt tacatacatg gacaatgtgg caataaagag gaggctgaag
    13141 cttttgctaa tattgcagtt ccacatgagt gtcaagtttt aagtgttgat ttgcctgaac
    13201 atggagaacg taaaagagaa aaaaattcat ttaacccatg gcatgttgtt ccagaattag
    13261 tatctattat ggaatatgca aagtgccact ggaatcaaat ttcgttatat gcaaatagca
    13321 ttggagcatg gtttagtatg ttaagctttg aaaaagagaa cttaagagag tgtatttttg
    13381 tatctccaat tctagatatg caacagctca tttcaaatat gatgctctgg gcaaatgtat
    13441 cagaagaaca gcttaaacat gaactaatta tcccaacttc atttgggcat actctttcat
    13501 gggaatatct acaatatgta aaacaacatc ctatcactca atggaatata ccaacaaaaa
    13561 tattatatgg taaacatgat gaacttactg aaatcggtgt tgtagagaaa tttgtagaaa
    13621 agtttaattg caaccttact attgtagaaa atggcaaaca ttggtttaac acaccacaag
    13681 aattaaattc attatatgaa tggttaaata ctttagttaa ataaaaaata attatattat
    13741 taatatgatt aaattattta taaatgttat ttaattaatt ttattttagc attattaaaa
    13801 taactacata aagaataaat tagttattat taaagaaatt aaaagtatat agagtattaa
    13861 taatttttta ataattagtt attatagaaa aaagagggat ataatattgt ctccctctct
    13921 ttcttctgta atattatata atttttattt atcactctca ataaattata gttatctaca
    13981 aaattcaatc tttaatacat tattaatata ttgtgatatt aatattacag tattaaataa
    14041 aatctaataa aattaactat aaaatatcta ttatataaat atccactctt atgtttgaaa
    14101 tccgt
//
LOCUS       DAEPIN010000087        13837 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.087, whole genome shotgun sequence.
ACCESSION   DAEPIN010000087 DAEPIN010000000
VERSION     DAEPIN010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13837
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.087"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            <1..757
                     /locus_tag="KR250_003183"
     CDS             <1..757
                     /locus_tag="KR250_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBH0069077.1"
                     /translation="KATVNIIENIGIFIPMGIFLPIVCKNLNKKTIIIVIILVSLAFE
                     LTQYIFALGSSDIDDVILNSLGGIIGINIYINMNKLFPNDINRFKAIIGTSLVLGVIG
                     LGVISKNYHNLLTFKFRPDRKISKILIEENREIIKDINKDTVDIVGTFESFKKGIITI
                     KAGSNNKVKRPENLIDSDGNIRIYLNENTKLVSYIITKESKMDIVKYEEFDVKNLDLL
                     RRYDTINVWIDKENQPKDKHGIMASKLLIGLYE"
     gene            872..1657
                     /locus_tag="KR250_003184"
     CDS             872..1657
                     /locus_tag="KR250_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069078.1"
                     /translation="MEIIKYILYFFIYSFLGWTVESIGCSIASKRIINRGFLNGPICP
                     VYGFGAVIVISLLGRFNNVVIVFLLGMILTTILEYFTGFILETLFHAKWWDYSDKKFN
                     IKGRVCLKNAIYFGVMSVLIIRFIHPFIKYFVSIIPYRILISMAIITTLWTILDLIVT
                     IITLKKLDIKLNLLDDIITDLNDINVKLDKFDRGEIQALFKTINNDELEVREKINKIN
                     SKLDRIESNIILQKRVIKAFPHIKHKKQQEQLEHFKKIINEKR"
     gene            1949..3268
                     /locus_tag="KR250_003185"
     CDS             1949..3268
                     /locus_tag="KR250_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBH0069079.1"
                     /translation="MSEITSQMISTEEKKYVAKTQKIPYYPVAFKSGDGAMLYDYEGN
                     EYVDFLASAGSANVGHGNKEISQAVKEQMDDITQYTLAYFHSDPPVKLAEKLVEIAPG
                     DNDKKVLYSATGSACIDAAIKLARGYTGRTKIISMCESYHGSTYGAISISALSTNMRR
                     KMGPLLPEVYHFHYPDKNRTAKECLDEIEYAFAHYLPAEEVAAIFIEPIAGDAGIIVP
                     PVEWVQGLSKICKENGILLVSDEIQQGMGRTGKWFGIENFGVEADLIVLGKSVGGGLP
                     LGAVVGRTEIMQSLDAPAHLFTLAGNTTVCVAALKSIEIIEKENLLQKSIEMGDYIKA
                     GFEKLKEKYDIIGEIRGIGLSIGVDIVKGKGSNEKHPDATAKICYRCIQTGLIMIFLG
                     QSTLRVQPPLVITKEQVDKAMNIIDSAIDDYLNGRIGDEVYEVTQGW"
     gene            complement(3343..3771)
                     /locus_tag="KR250_003186"
     CDS             complement(3343..3771)
                     /locus_tag="KR250_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069080.1"
                     /translation="MKDNNNTNKTIQFGEKKASLENNIQKNKNIKLDNTHKVIPYPKN
                     RKKDDKKKGNKKKNTNTSKVNKNNYGHSFFNNKYKYKRKPYVIALYTVILILLAILII
                     TKLTDSTNKGNYSRNTVSDTHKNDIRNIVFNESNNFLICG"
     gene            complement(3862..5238)
                     /gene="hydG"
                     /locus_tag="KR250_003187"
     CDS             complement(3862..5238)
                     /gene="hydG"
                     /locus_tag="KR250_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HBH0069081.1"
                     /translation="MFINHELINSLLEDAKNSTSDDIEKVLDKADRREKLSYKDIATL
                     LEVEDKKQLDRLFSIAGQIKNEIYGNRVVLFAPLYVSNYCVNECVYCGFSKCNKFKRK
                     KLTMEEIREEVKILEKMGHKRLALEAGEDPKNCDINYILDCLDAIYSTYNENGNIRRV
                     NVNIAATSVDEYKLLKEKGIGTYILFQETYHKPTFIKMHGQSIKNDYYYHLTAFDRAM
                     EAGVDDVGAGVLFGLSDPKFEVLGLMMHNEHLEEKFGVGFHTISFPRLKKAEGMSLED
                     FPHLVSDDMFKKIVAITRLAVPFTGIIMSTRETAEMRNELLKYGVSQISAGSLTGVGG
                     YKAYEDGDNTEQFEVGDHRSPVEVLKELITDGYIPSYCTACYRKGRTGDRFMSLAKSR
                     QIHNVCTPNALTTLNEFLIDYGDEELKIMGKKLIAEEIGKIEREDIRNIVSNNMTALE
                     RGERDLYL"
     gene            complement(5239..6324)
                     /gene="hydE"
                     /locus_tag="KR250_003188"
     CDS             complement(5239..6324)
                     /gene="hydE"
                     /locus_tag="KR250_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HBH0069082.1"
                     /translation="MTKNKIKYFIDKLYSINSLSFDELLYLIENIDNEEYNFEQNLRN
                     YLYKQASDMRNKHYGNRVYLRGLIELTNYCKNNCYYCGIQSSNKNIIRYRLSLDEILE
                     CCDIGYKIGYRTFVLQGGEDAYFTDDKICEIVSSIKNKYEDCAITLSLGEKSYDSYKK
                     YFLCGADRYLLRHETATDSHYKKLHPPNIELRTRKECLKNLKEIGYQVGAGFMVESPF
                     QNNEDLVRDLLYLKELNPHMVGIGPFIPHHDTIFKDYKHGDLEKTLLMLSITRLLLPK
                     VLLPATTALASINPSGRNAGLLAGCNVIMPNLSPQEFRKQYSLYDNKAFTGQEACEYH
                     QSLEENIKSLGLEVDYSRGDNIEWRRL"
     gene            complement(6360..7568)
                     /gene="hydF"
                     /locus_tag="KR250_003189"
     CDS             complement(6360..7568)
                     /gene="hydF"
                     /locus_tag="KR250_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HBH0069083.1"
                     /translation="MSLNSTPQSVRVHIGLFGKRNAGKSSIINAITNQSAAIVSDIAG
                     TTTDPVFRPMEILPIGPCVLIDTAGLDDVGELGELRIGKSLDVLEKTDIALLVVDCQI
                     GISQEDLSLIEKFNDKNIPHILILNKIDTIKNQSEILNLTKNKVKCPVVSVSSTDKIG
                     IENLKNEIIKVLPKDSTEFKLVSDLIEPNDLIVLVVPIDKAAPKGRLILPQQQVIRDI
                     LDSGAISIVTKEDSLKETLSNLGKKPKLVITDSQVFPQVDKDTPKDIPLTSFSILFAR
                     QKGDLKELINGAYALENLKDGDKILMAEGCTHHRQTDDIGTVKIPNMIRKKTGKNITF
                     EFSSGVSFTEDINKYALVVHCGACMMNRAGMLSRIEKAKSFNVPIVNYGILIAYVKGI
                     LERSLELFNY"
     gene            complement(7583..7834)
                     /locus_tag="KR250_003190"
     CDS             complement(7583..7834)
                     /locus_tag="KR250_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003361768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-only hydrogenase system regulator"
                     /protein_id="HBH0069084.1"
                     /translation="MKKVAVISAILEEPKECQFKFNEVVSGFKGIIKGRMGIPCEEEG
                     VSIVCIAVVGEMDTINSLTGKLGNIPHVSVKTSISKKEV"
     gene            complement(8215..9255)
                     /locus_tag="KR250_003191"
     CDS             complement(8215..9255)
                     /locus_tag="KR250_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HBH0069085.1"
                     /translation="MNLNLTNTINLMKKYLDIPSPAGYTENAVLEIKKDFENLGLDTI
                     LTKKGALIATLPGEDDSNQITISAHMDTLGAIVKEISSDGTLKYHKVGGGCWAAIEGE
                     NCTVITRKGKKIRGSVVFKYASTHIYGQTKAATERNEETMIIRLDEKVFTKQDVLDLG
                     ISVGDFVCLDHRFEATESGFIKSRYIDNKSAVAMVLEICRYFKENNLTPKHTTNFYIS
                     NYEEIGHGVSKCLPEKTKEFVAIDIAPTGPSQTSSEHGVTIVAKDNLSLYDFNLRNKL
                     VDVAEDNNLDYAVDVFTFYGSDASEAIRWGEDVQIACVGPGTNNSHHYERTHIEAIEN
                     TLKLLINYMLKE"
     gene            9725..10885
                     /locus_tag="KR250_003192"
     CDS             9725..10885
                     /locus_tag="KR250_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HBH0069086.1"
                     /translation="MIETGFVYCSFLVVFVGVIFFMVDKLKWKVFDVINPMLLIYLGS
                     AILGSFGLFAQNEDVGTYQNIITVNFLPLMLVFLLIQCDLRKILKMGPKMLLSFFCAT
                     FTFMLGFVIAFIIFKNSMGADSWKILGAASGSWIGGSSNMIAAANALGVAEEGLSYAI
                     LMGSIGYTVWMSVCLMSVKFGPKFNKWTKCDTSFIDEVTSKLEKENKDNTAPTFVELM
                     ILLSVGFGVASFAQIVSKILPTSGILNETMWVVLVASTVSILLGMTKISKLKGSTLVG
                     NVFMYLLLAATGSKANFFGLSEAPVYIMFGLVVVLVHAISFIGLAKLFKLDLFTCEVG
                     SIANIGGVTTAPMIAGVHNPVLIPVGVLMGLLGNIIGTYCALMVTQILHMLA"
     gene            11228..11389
                     /locus_tag="KR250_003193"
     CDS             11228..11389
                     /locus_tag="KR250_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069087.1"
                     /translation="MIIPSRRIQDITLTNLKNGEVTLIELDEIYKKMGFLFVVSEGKL
                     KKIKKENHH"
     gene            11582..12211
                     /locus_tag="KR250_003194"
     CDS             11582..12211
                     /locus_tag="KR250_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HBH0069088.1"
                     /translation="MKIVTYNIHKGMDSNNRLTLTKMGLYLKQLDCDVICLQEVLYPQ
                     FLALKAVLNMGGVFATNVKKVNMIYGICTFTTSKMLNSNHFFLTSKKEQRGALCITID
                     AYGRIINVINTHLGLDRQERAKQLDEIIDYRNRLVGIVILCGDFNEKNVFLSMFNDMA
                     ISLNKSYLPTFEKSNSRIDYIFVNKNTELKGYTVEKIYLSDHYPVIGYI"
     gene            12451..13710
                     /locus_tag="KR250_003195"
     CDS             12451..13710
                     /locus_tag="KR250_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HBH0069089.1"
                     /translation="MGRRTTRVSRLGKEKKRSVLIVLAALVLIMGGFTYFFNSKFLYN
                     GKIAKNVYIEGVNVSDMTKAEALKAITDKYTPEDLNLAYDGKKYTISPKDIDLKYDTE
                     DVVKDAYESTKKGSYFQNLKKYIDIRVNKANMKIKAEYDEAKLSSKVSSIADSINVKM
                     KNASISVGSGGLSYTDSVVGREFDLAANKESIYNMIKNKEHKTLELKVNLQKPDITTE
                     QVKTVNSVIGQYSTTYSQAVEGRSYNVGLSARKTSDVLLMPGEEFSYNKLTGPSNKAN
                     GYKDAPVIVYGKLEQSAGGGVCQTSSTVYNAALLSGMEITQVTNHSSASTYVPKGRDA
                     TVSDGGLNLKFKNPYKHPVYIKNYAGGGSVSSVIYGNSGDKPNISIEVKQTGQNKYST
                     YRIFKDSSGKVIKKEHISNSSYKELKK"
BASE COUNT         5072 a         1760 c         2082 g         4923 t
ORIGIN      
        1 gaaagctact gtaaatatta tagaaaatat aggtatattt attcctatgg gaattttttt
       61 acctatagta tgtaaaaatc taaataaaaa aactataatt atagtaatta tacttgttag
      121 cctagcattt gaattaacac aatatatttt tgctcttgga agtagtgata tagatgatgt
      181 tattttaaat tcattaggtg gcataattgg aattaatatt tacataaaca tgaataaatt
      241 atttcccaat gatattaaca gatttaaggc tataataggt actagtttag ttttaggagt
      301 tataggatta ggagttattt ctaaaaatta tcataactta ctaactttta aatttagacc
      361 tgatagaaaa atatcaaaaa tattaattga agaaaatagg gaaataatta aagatataaa
      421 taaagataca gtagatatag ttgggacatt tgagagcttt aaaaaaggaa taataactat
      481 taaagcaggt tctaataaca aagtaaagag accagagaat ttaattgatt ctgatggaaa
      541 tataagaatt tatttaaatg aaaatacaaa attagtatct tatataatta ccaaagaatc
      601 aaaaatggat atagttaaat atgaggaatt tgatgtgaag aatttagatt tattaaggag
      661 atatgatact attaatgttt ggattgataa agaaaatcag ccaaaggata aacatggtat
      721 tatggctagt aaattattaa ttggattgta tgaataaaat attaaacaag aataaaagaa
      781 taacaaatat aaaaaacgaa aaaactattt ctataattat ttttacgttg tgcataaaat
      841 aagaacatta gtgaaggagt agagataaat tatggagata ataaaatata tcttgtactt
      901 ttttatatat tcatttttag gatggacagt agaaagtata ggatgttcta tagcaagcaa
      961 aagaataatc aacagaggtt ttttaaatgg tccaatatgc cctgtttatg gttttggagc
     1021 ggtaattgtg atatcactat tgggaagatt taataatgta gtaatagtat ttctattagg
     1081 aatgatttta actacgatac tagaatattt tacaggattt attttagaaa ccttatttca
     1141 tgctaaatgg tgggattatt ctgacaaaaa attcaatata aagggtagag tatgtttaaa
     1201 aaatgcaata tactttggtg taatgtctgt tttaataata agatttatac atccatttat
     1261 aaaatatttt gtaagtataa ttccatatag aatattaatt tcaatggcta taattacaac
     1321 actttggact atacttgatt tgattgttac tattataaca cttaaaaaat tagatataaa
     1381 gttaaactta ttagacgata taattacaga ccttaatgat ataaatgtga aattggataa
     1441 atttgataga ggagaaatac aagctttatt taaaacaata aataacgacg aattagaagt
     1501 tagagaaaaa ataaacaaaa taaatagtaa gttggaccgt atagagtcta atattatttt
     1561 acaaaaaaga gttataaaag cttttccaca tataaaacat aaaaaacaac aagagcaact
     1621 agaacatttt aaaaaaatta ttaatgagaa aaggtaatta tacaatttaa tgaagacgaa
     1681 tagaataatg gtatttataa gatgattttc agaggactct ataaaaatag aatagagtcc
     1741 tttatttttt gaaaaataat gtttatttga taaaaataaa atatattgtt tctgaataac
     1801 aaatattttg aaaattataa aaacatatat tgacaatttg tgtcaaaaat actaacatta
     1861 agtatataaa gaattttata tgagaatatc tttataaatc gcaataattt taaaacataa
     1921 atttatttaa agaggggatg atttgccaat gagcgagata actagtcaaa tgataagtac
     1981 agaagaaaaa aaatatgttg ctaaaactca aaagatacct tattatccag tagcatttaa
     2041 atcaggtgat ggtgcaatgc tatatgatta tgaaggtaat gaatatgtag attttttagc
     2101 aagtgcaggc tctgcaaatg taggtcatgg taataaggaa atctcacaag cagttaaaga
     2161 acaaatggat gatataacac aatatacact tgcatatttc catagtgatc ctcctgtaaa
     2221 attagctgag aagcttgtgg aaatagcccc tggagataat gataaaaaag tactatacag
     2281 tgcaactggt tctgcttgta tagatgctgc tataaaatta gcaagagggt atactggaag
     2341 aacaaagata atatctatgt gtgaatctta tcatggaagt acttatggag ctatatctat
     2401 atctgcttta agtacaaata tgagaagaaa aatgggtcca ttgttaccag aagtttatca
     2461 cttccattat cctgataaaa atagaacagc taaagaatgt ttagatgaga tagaatatgc
     2521 ttttgcacac tatctaccag cagaagaggt agctgcaata tttatagagc caattgcagg
     2581 agatgcagga ataatagtgc ctccagtaga gtgggtacaa ggtttaagta aaatatgtaa
     2641 agaaaatgga atattacttg taagtgatga aatacaacaa ggtatgggaa gaacaggtaa
     2701 gtggtttggt atagaaaatt ttggtgttga agcagacctt atagttcttg gaaaatctgt
     2761 tggaggtgga ttacctctag gagcagttgt tggaagaaca gaaataatgc aaagtcttga
     2821 tgcaccagca catttattta ctttagctgg aaacacaaca gtatgtgtgg ctgctttaaa
     2881 atctatagag ataattgaaa aagaaaactt acttcaaaag agtatagaaa tgggagatta
     2941 tattaaagca ggatttgaaa aactaaaaga aaaatacgat ataattggag aaataagagg
     3001 cataggatta tctataggag ttgatatagt taaaggaaaa ggttctaatg aaaaacatcc
     3061 agatgctaca gctaagatat gttatagatg tatacaaact ggattaataa tgatattctt
     3121 aggacaatct actttaagag tgcaaccacc tctagtaata actaaggagc aagtagataa
     3181 agcaatgaat atcatagatt ctgcaataga tgattattta aatggtagaa ttggtgatga
     3241 agtatatgaa gtaactcaag gttggtaaaa taaatattaa atgaaaatag gagttatctc
     3301 aattttagaa aattatttct gaaaagagat aactctttta agttacccac aaataagaaa
     3361 attgtttgat tcattaaaga ctatatttcg gatgtcgttt ttgtgagtat cagagacagt
     3421 attacgagaa taattacctt tattcgtact atctgttaat ttagttataa ttaagatagc
     3481 taaaagaata agaattactg tgtataaagc aataacatat ggttttcttt tatatttgta
     3541 tttgttattg aaaaaagaat ggccataatt atttttattt actttagatg tatttgtatt
     3601 ctttttcttg ttacctttct ttttatcatc cttttttcta tttttagggt aaggaatgac
     3661 tttgtgtgta ttatcaagtt tgatattttt atttttttga atattgtttt ccaggctagc
     3721 ttttttttct ccaaattgaa tagttttatt ggtattgttg ttatctttca atgttaatct
     3781 ccctccctta aactttccct ttagataata ttactactaa cctatggaat atacaataaa
     3841 taatatataa gtaataaaat tttataggta taaatctctt tctccacgct ctaaggcagt
     3901 catattgtta gatactatgt ttcttatatc ttctctttct atcttaccta tttcttctgc
     3961 gataagtttt ttacccataa ttttaagttc ttcatctcca taatcgatta aaaattcatt
     4021 taatgtagta agagcatttg gtgtacatac attatggatt tgtcgacttt tagctaagct
     4081 catgaatcta tcgccagttc tacctttacg ataacaagct gtacaataac taggtatata
     4141 tccatcagta atcagttctt taagcacctc aacagggctt ctatggtctc caacttcgaa
     4201 ttgttctgta ttgtcaccat cttcatatgc tttatatcct ccaacacctg ttagagaacc
     4261 tgcactaatt tgtgatacac catattttag taactcattt ctcatttcag cagtttctct
     4321 agtggacatt atgattccag taaaaggaac agctaatcta gttatagcta ctattttttt
     4381 gaacatatcg tcagaaacta agtgaggaaa atcttctaag ctcatacctt cagctttttt
     4441 caatctaggg aaagatatag tatggaaacc tacaccaaat ttttcctcta agtgttcgtt
     4501 gtgcatcata agtcctagaa cttcaaattt agggtcagat aaaccaaata aaactcctgc
     4561 accaacatca tcaacaccag cttccatagc tctatcaaaa gctgttaaat ggtaataata
     4621 gtcatttttt atagattgac catgcatttt tataaatgtt ggtttgtgat aagtttcttg
     4681 gaataatata taagttccta ttcctttttc ttttagtaat ttatactcat ctacacttgt
     4741 agctgctatg ttaacgttta cccttcttat atttccgttt tcattatatg ttgaataaat
     4801 tgcatctaag caatctaaga tgtaatttat atcacaattt ttagggtctt caccagcttc
     4861 tagagcaagt cttttatgac ccattttttc taatatttta acctcttccc ttatctcttc
     4921 catggtaagt ttttttcttt taaatttgtt gcatttacta aaaccgcaat aaacacattc
     4981 atttacacag tagtttgata cataaagagg ggcaaataaa accactctat taccatatat
     5041 ttcatttttt atttgcccag ctatactaaa taatctatct agttgttttt tatcttctac
     5101 ttcaagaagt gtagctatat ctttataact aagtttttcc cttctatcag ccttatcaag
     5161 taccttttca atatcatcag aagttgaatt ttttgcatct tctaataaag aattaataag
     5221 ttcatggttt atgaacattt acaaacgcct ccattcaata ttatcaccgc gagaatagtc
     5281 aacttcaagt cctaatgact tgatgttttc ttctaaactt tggtgatatt cacaagcttc
     5341 ttgaccagta aaggctttgt tatcgtaaag agaatattgt tttctaaatt cttgaggaga
     5401 aaggtttggc ataattacat tgcaaccagc taaaagacct gcatttctac cactagggtt
     5461 tatagatgcc aaggctgttg tagcgggtaa taaaactttt ggaagtagaa gtcttgtaat
     5521 tgaaagcata agtaatgtct tctctaaatc tccatgttta taatccttaa aaattgtatc
     5581 atggtgtgga atgaaaggtc ctattccaac catatgagga tttaactctt ttaggtataa
     5641 taaatcacga acaaggtctt cgttgttctg aaatggtgat tcaaccataa atccagcacc
     5701 tacctgataa ccaatttctt taaggttttt taggcattct tttctagttc taagctcaat
     5761 gtttggcgga tgtaattttt tataatgact gtctgtagca gtttcatgtc ttaaaagata
     5821 tctatctgca ccacataaaa agtatttttt atacgaatca taactctttt ctcctagtga
     5881 tagagttatg gcacaatcct catatttatt ttttatactt gaaacaattt cacatatttt
     5941 atcatcagta aaatatgcat cttcaccacc ttgcaaaacg aatgttctgt atcctatttt
     6001 ataacctata tcacaacatt caagtatttc atcaagtgaa agcctgtatc ttatgatatt
     6061 tttattagaa gattgtattc cacaatagta gcaattattt ttacaatagt ttgtaagctc
     6121 tataagtcct ctcaagtaga ctctatttcc ataatgttta tttctcatgt cgctagcttg
     6181 tttataaaga tagtttctta aattttgttc gaagttatat tcctcattgt cgatattttc
     6241 tattaaatat agtagttcat caaaacttaa tgaattaatg ctgtaaagtt tatctataaa
     6301 gtatttaatc ttatttttag tcatattcaa atatataatt attaagtata gttaaatttt
     6361 taataattga aaagttcaag tgatctttcc aaaatacctt ttacatatgc tattaagata
     6421 ccatagttta caataggtac attgaaagat tttgcctttt ctattctaga taacattcca
     6481 gctctattca tcatacaagc tccacagtga actacaaggg catatttatt tatatcttca
     6541 gtgaaggata ctccagaact aaattcaaaa gtaatgtttt tgccagtttt ctttctaatc
     6601 atattaggaa tttttactgt tccaatatca tcagtttgtc tatgatgggt acatccttcg
     6661 gccatcaaga ttttatcacc atcttttaaa ttttctaaag cataagcgcc attaatcaat
     6721 tctttaaggt ctcccttttg tctagcaaac aatatagaaa aagatgtaag aggaatatct
     6781 tttggtgtat ctttatctac ttgtggaaat acttgagagt cagtaataac aagttttggt
     6841 tttttgccta aattagatag agtttctttt aaactatctt cttttgtaac tatagaaatt
     6901 gcaccactat ccaaaatatc tcttataact tgctgttgag gaagtataag tctacctttt
     6961 ggagctgctt tatctattgg aacaactaaa acaattaaat catttggttc aattaaatct
     7021 gataccaatt tgaactcagt gctatcctta ggcaaaactt ttattatttc attttttaaa
     7081 ttttcaattc caattttatc tgttgatgag acagaaacaa ctggacattt aactttattt
     7141 ttagttaagt ttaaaatttc tgattgattc ttaatagtat caattttgtt gagaattaga
     7201 atatgaggga tattcttatc attaaacttt tcaattagtg acaaatcttc ttgtgatata
     7261 ccaatctggc aatccactac tagtaaagct atgtctgtct tttctaaaac atctagtgat
     7321 ttacctattc taagttcacc aagttcacca acatcatcaa gaccagcagt atctataaga
     7381 acacaaggtc ctataggaag gatttccatt ggtctaaata ctgggtctgt agtagttcca
     7441 gcaatatctg agactattgc agcactttga tttgttatag catttattat gctagattta
     7501 cctgcatttc ttttaccaaa gagacctata tgtactctta ccgattgtgg agtagaattt
     7561 agactcattt gtactcacca ccttaaactt ctttttttga tatactagtt tttacagata
     7621 catgaggtat gttgcccaat ttaccagtca aactattaat agtatccatt tcgccaacaa
     7681 ctgctataca tacgattgag acaccttctt cttcgcaagg aatgcccatt cttcctttaa
     7741 taataccttt aaaacctgat acaacctcat tgaatttaaa ttgacattct tttggctctt
     7801 ccaaaatagc acttataact gcaacctttt tcataaatat cctcctaact cagaaaatat
     7861 gtcttaaaca aatccaaaat caaaaaactt ttctttgtaa agaaaagttt ctacaaaaga
     7921 aaagttatta attacacaag tataatgtgc tatattaaac tatttcctta cagattagat
     7981 aactcttttc tggtagggtt agcttgtaaa aatttaattt aacaacaagt aactgaagaa
     8041 aatttttata caatttatat aaattataat aaaaattaac taaagaaaat tgaagttact
     8101 tatataatag tagtaaattg aaattaagtc tatagaaaaa ccaatttttg tataaaaata
     8161 aaaattaaag ttaattattt gttaagaatt gactttggta aaatatataa ttaattattc
     8221 tttaagcata taatttataa gtaattttag tgtattttca atagcttcta tatgtgttct
     8281 ttcataatgg tgactattgt ttgttccagg accaacacat gctatttgaa catcttctcc
     8341 ccaacgaata gcttctgaag catctgaacc atagaaagta aatacatcga cagcgtagtc
     8401 taaattattg tcttctgcaa catcaacaag cttgtttctt aaattaaaat catataatga
     8461 taaattatct tttgcaacta tagttacacc atgttcacta gatgtttgac ttggtccagt
     8521 aggtgctata tctattgcta caaattcttt tgttttttca ggtaaacatt tagaaacacc
     8581 atgtccaatt tcttcataat tacttatata aaagttagta gtatgcttag gtgttaaatt
     8641 gttttcttta aaatatctac atatttcaag aaccattgct acagctgatt tattatctat
     8701 atatctagat tttataaaac cagattctgt agcctcaaat ctatggtcta ggcatacaaa
     8761 atccccaaca cttattccta aatctaatac atcttgcttt gtaaatactt tttcatcaag
     8821 tctaataatc attgtttctt catttctttc agtggcagct tttgtttgac catatatatg
     8881 agtagaagca tacttaaata caacacttcc tctgattttt ttaccttttc tagttataac
     8941 agtacagttt tctccctcta tagctgccca acatccacca ccaactttat ggtatttaag
     9001 agtaccatca gatgatattt cttttactat agctccaagt gtatccatat gtgcagatat
     9061 tgttatttga tttgaatcat cttcaccagg aagagtagct ataagtgcac ctttttttgt
     9121 aagaatagtg tcaagaccta aattttcaaa atcttttttg atttctaaaa ctgcattttc
     9181 agtatatcca gctgggcttg gaatgtccaa atattttttc attaaattta tagtattagt
     9241 taaatttaaa ttcatattgt ttcctcctaa ataaatatat ttatacaaat tataacataa
     9301 atacaaatgt tctgaaaact aaaattaaaa gaatagaatc taaacatatt taaaaaaaaa
     9361 gtatataaaa taaatttgat ttatattata tagatttaaa gtaaaaatag ttttatttaa
     9421 taattaataa aaataaatac aaaacaaaat aaataaataa taaaatattt agtttaatta
     9481 tctttacaaa taaaaatttt agtaataaaa taattgttga aaaagatttt aaattcagat
     9541 tattcatttc taaagtaatt aatgtgcaaa tctttattaa tatatgtcaa tagttaggta
     9601 tatatgtatt taattagcat gatatagata aaagtattta ttttaattgt ctattatctg
     9661 aaaaagtctt tacttagaaa tcataaaaat caaattaatt aggaaaagaa agggtgtgta
     9721 taatgtgatt gagactggat ttgtatattg tagtttcttg gttgtattcg taggtgtaat
     9781 tttttttatg gttgataaat tgaaatggaa ggtatttgat gttattaatc caatgctttt
     9841 aatatactta ggttcagcta tacttggatc atttggttta tttgcacaga atgaagatgt
     9901 tggaacgtat caaaacataa taacagtgaa cttccttcca ttaatgttag tgtttttgtt
     9961 aattcaatgt gatttaagaa aaatattaaa gatggggcct aaaatgttat tatcattttt
    10021 ctgtgctaca tttacattta tgcttggatt tgttatagca ttcataattt ttaaaaattc
    10081 aatgggtgca gattcatgga aaattcttgg ggcagcatct ggttcatgga taggtggctc
    10141 atcaaatatg attgcagcag ctaatgctct aggagttgca gaagaaggac ttagttatgc
    10201 aatacttatg ggttctatag gatatacagt atggatgtct gtttgtctaa tgtctgtgaa
    10261 atttggacct aaatttaaca aatggacaaa gtgcgataca agttttatag atgaggttac
    10321 atcaaaactt gaaaaagaaa ataaagataa tacagcacct acgtttgtag aacttatgat
    10381 tttactttct gttgggtttg gagttgcgtc ttttgcacaa attgtatcca agatacttcc
    10441 aacttcaggt atacttaatg aaactatgtg ggtagtactt gttgcatcta cagtatcaat
    10501 attattgggt atgacaaaaa tatcaaagct taaagggagt acactggtag gaaatgtgtt
    10561 tatgtattta ctccttgcag ctacaggtag taaagcaaat ttctttggat tgtcagaagc
    10621 acctgtttac ataatgtttg gattggtagt agtccttgta catgctattt catttatagg
    10681 gcttgctaaa ctatttaaac ttgatttatt tacttgtgaa gtaggaagta tagctaatat
    10741 aggaggagta actacagctc caatgattgc tggggttcac aatcctgtat taataccagt
    10801 aggtgtactt atggggcttc taggaaatat tatagggact tattgtgctc ttatggtaac
    10861 tcaaatatta catatgttag cttaatgaaa atatggtata aaattaataa tttaaataag
    10921 aaaagtcatt tcaatttaag cagaagcttt tactgaaatg gctttttttg tttgacaaga
    10981 taatctattt caagaagatg ttttgacata tttagtgtat aatattgtaa aatttactaa
    11041 aaacgagata tatttaattt ttatattgaa tcgaacgtct gtatgatgta taataaatat
    11101 acagaacgaa tgtttgcgga acgggtgttt tttgaattta ttttaataat ttatgaatat
    11161 atatcagaat gcataaatat atttaaatat aaagattaaa aagaatttaa aggagtgttg
    11221 ataacttatg ataattccat caagaagaat acaagatata acattaacaa atttaaaaaa
    11281 tggtgaagta actttaattg aattggatga aatatacaag aagatgggat ttttatttgt
    11341 tgttagtgaa ggcaaattga aaaagataaa aaaggagaat catcattaga aatgaatgaa
    11401 ataataaata ttataatagt ttataaaacc taacttatgt agaatataaa tacttaagtt
    11461 aggttttttt gtgataaagt ttataagtac tcaattttat aagataaata atggttttaa
    11521 gtgaaaagta aataagttta aattttagta agtattatat tgcataagag gtgatattat
    11581 tatgaaaata gtaacctaca atattcataa aggtatggat tcaaataata gattaactct
    11641 aactaaaatg gggttatatt taaaacaatt ggattgtgat gtaatttgtt tgcaagaagt
    11701 attatatcct caatttttag ctttaaaagc tgttttaaat atgggtggtg tctttgctac
    11761 aaatgtaaaa aaagtaaata tgatatatgg tatttgtaca tttacaacat ctaaaatgtt
    11821 aaacagtaat cattttttct taactagcaa aaaagaacaa cggggagctc tttgtataac
    11881 tatagatgca tatggaagaa ttataaatgt aataaatact cacttgggac ttgataggca
    11941 ggagagagca aaacaattag acgaaataat agactatagg aacagattag tggggatagt
    12001 tattttatgt ggtgatttta atgagaaaaa tgtcttttta agtatgttta atgatatggc
    12061 tatttcacta aacaagtctt acttaccaac atttgaaaaa tcaaactcta gaatagatta
    12121 catttttgtt aataaaaata ctgaactaaa aggatacaca gtagaaaaaa tttacctgtc
    12181 agaccattat cctgtaatag gttacattta aatttcaata taaatgaaat tagttgttat
    12241 aaaataaaca atttttttgt taagaaatag taaaagaaat ataataaaaa atatcaaaaa
    12301 taaaactaat tataagacat aaatttacaa taaagttaca tttgattaaa ttccttgttt
    12361 aacaaaagct attcaaatat aatctaaggt agttttgaaa tgggaactct attatgcaaa
    12421 tttaagctag tgttaaaatg aggtgaagtt atgggcagaa gaacaacaag agtttcaaga
    12481 ttaggtaaag aaaaaaaacg aagtgtttta attgtattgg cagctttagt tctaataatg
    12541 ggtggattta cttatttttt taacagtaaa tttctgtaca atgggaaaat cgctaaaaat
    12601 gtatatatag aaggagtaaa tgtatctgat atgactaaag cagaggcatt aaaagctata
    12661 acagataagt atactccaga agacttaaat cttgcttatg atgggaaaaa atatacaata
    12721 tcaccaaaag atattgattt aaaatatgat acagaagatg ttgtaaaaga tgcttatgaa
    12781 agtacaaaaa aagggtccta ttttcaaaac ttaaaaaaat atattgatat aagagtgaat
    12841 aaggcaaata tgaagataaa agctgaatat gatgaggcaa aacttagttc caaagtgtct
    12901 tctatagcag atagtataaa tgttaagatg aaaaatgctt ctattagtgt tggaagtggt
    12961 ggactaagtt atacagactc tgttgtaggt agagaatttg atttggcagc aaataaagag
    13021 tctatatata atatgataaa aaataaagag cataaaactt tagaacttaa agtaaatctt
    13081 caaaaaccag atataacaac tgaacaagta aaaactgtaa attctgttat aggtcaatat
    13141 tcaactactt attcacaagc tgtagagggt aggtcatata atgtaggatt atcagcaaga
    13201 aaaactagtg atgtattatt aatgccaggt gaagaatttt catacaataa acttacagga
    13261 cctagtaata aagcaaatgg ttacaaagat gcacctgtta tagtttatgg taagctagaa
    13321 caatcagcag gaggaggtgt ttgtcaaaca tcttcaacag tttataatgc tgctcttctt
    13381 tcgggtatgg agataactca agttacaaat cattcatctg catctactta tgtgcctaaa
    13441 gggagagatg ctactgtgag cgatggagga ttaaacttaa agtttaaaaa tccatataag
    13501 catcctgtat atattaaaaa ttatgcaggt ggaggaagtg tatcatcagt aatctatggt
    13561 aattctggtg acaaaccaaa tatatctata gaggtaaaac aaactggtca gaataaatat
    13621 tctacatatc gaatatttaa agattcaagt ggaaaggtca taaaaaaaga acatatatca
    13681 aatagttctt ataaagaatt aaaaaaatag ataatttttt agagaaatat ttgtaatata
    13741 aaagggagtg tctcaaaatg aactttttag tttatgaggc actctttttc tgtgaaatca
    13801 aatatatttt catcatttca acaatacaaa agaagcg
//
LOCUS       DAEPIN010000088        13751 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.088, whole genome shotgun sequence.
ACCESSION   DAEPIN010000088 DAEPIN010000000
VERSION     DAEPIN010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13751
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.088"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            135..275
                     /locus_tag="KR250_003196"
     CDS             135..275
                     /locus_tag="KR250_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069090.1"
                     /translation="MGIIESASKLAEMVHLLAVEKGITDIEAWDEAVKEYSKIYEERR
                     NE"
     gene            complement(450..770)
                     /locus_tag="KR250_003197"
     CDS             complement(450..770)
                     /locus_tag="KR250_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HBH0069091.1"
                     /translation="MKLKTISLPELNNLDPTLESTFIKMGEEQGELAECIGKFRNLSG
                     ENNDLDEVDIIKKTAKELMDVAQTCVTMMFKLEEQYGINLDEIRKEHIKKLEKRGYIK
                     NMDK"
     gene            complement(890..1627)
                     /locus_tag="KR250_003198"
     CDS             complement(890..1627)
                     /locus_tag="KR250_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HBH0069092.1"
                     /translation="MDANSLKDLIANHNNIVFFGGAGVSTESNIPDFRSSTGLFSQKL
                     NKQFTAEQLVSHTFFVRYPEEFFEFYKDKLIYPNAKPNNAHIALAKLEEMGKLKAVIT
                     QNIDGLHQMAGSKNVLELHGSVHRNYCTKCGKFFDLESMLNLGGNIPYCDNCGSIVKP
                     DVVLYEEALDSDVITKTISAISNADLLIIGGTSLAVYPAASFIDYYKGDYIALINKAN
                     TVYDKSASLVINKPIGEVLYEAVLRQI"
     gene            1842..3665
                     /gene="acd"
                     /locus_tag="KR250_003199"
     CDS             1842..3665
                     /gene="acd"
                     /locus_tag="KR250_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosaminidase"
                     /protein_id="HBH0069093.1"
                     /translation="MKKKAALATLAMLPLGVVNAHADGDIGIVTINYLNVRNEPTAES
                     SIAFVAKKDDKVLIKDSSNGWYKIKAESGQEGWASSKYIAKSNSDSLRTSTNKEKQVI
                     SNSLNMRNGAGTSYRVITVLKKGQKVEVISESNGWSKIKYDGRLGYVSSSYLGDVSNS
                     TNKSKTKQVNTTSLNVRSGPNTSYGLLGKLPKGSKVEVISESNGWSKIKYNGKDAYVS
                     SMYLSDVSQSNSDNSSQSNDKKNTDKVVNTASLNVRSGPGSTYSKLGKVYKGSKVTVL
                     SESSGWAKINFNNKEAFVVGNYLSTSADTSNNNSNSNSDNSSNSNGNNSSSSGQVNGM
                     SGISGAKIDYKSLSYTLESHISKQVEKAASGGNVIAPSNRKSTPSPEFSTFSAQRTSS
                     FVNASSSDIEYYLNPKNFTNTTKGMMQFLKINSYRDGISESSLNSYLNGLSSSVFKNQ
                     GAAFINAAKKYNIDVVYLVSHAMWETAYGKSTLAQGQTLTSYKGQPLSKPVKVYNFFG
                     IGAIDKSANVSGAEAAYSNGWTSVEATIDGSAKWISQNYVNSSKYNQNTIYKMKWNYD
                     YTWHQYATDVNWANGISGIMENLIGLYGGGSSLVFEVPQYK"
     gene            3880..8178
                     /locus_tag="KR250_003200"
     CDS             3880..8178
                     /locus_tag="KR250_003200"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HBH0069094.1"
                     /translation="MESIKEYLDKLEINNSGLGKQLKEVYINRVVYFKEDKIVYFYLT
                     SKDIVSHELLDKFKEELMYKLDYFKDMRMKIRFTGLERKSNKDVIKKYWNNILYILKY
                     LCPSIAGWYKQVEFLCLEEELKIKLPKGIFYERLMKKNVTYVLKTVLSEELGLDLNIT
                     IEKAVDEKVNKERLIRINDREMEEKIRALEIGKVNNCEENEEESYVIKSDVDENLIYG
                     DNANAMIENIVELNASSGTVAVVGDIFDVDTKELKNGKILMIASITDYTSSISCKLFL
                     TDTNKDGVLENVKKGAYLKIKGDIVYDTYQREISMMISGIRKETRIEREDKSEEKRVE
                     LHAHTQMSSMDAICSVKKLVERAAKWGHSAIAITDHGVVQGFPDAMNAGKSNNIKILY
                     GVEGYLVEDDSLIIEDANDKELSQTFVVFDIETTGFSNTNDKITEIGAVKIENFKIVD
                     RFSELINPEKDISYKIQELTGITNELIKDKPTIEEVLPKFMEFVGDSVLVAHNAEFDT
                     GFISQKCREQGLLYNNKKVDTLMLARVMLPNLKRHRLNVVAKELGIPLLNHHRAVDDA
                     EATALIFNKFLQMLTEKGAKTLSDVNNILGKIDYTKLSTNHITLIAKNSIGIKNLYKI
                     ISDAHVNHFFRAPRILRSVLNEYKEGLIIGSACEAGVVFQAVKKNVSDEEMKKIIDLY
                     DYIEVMPIDNNRFMIDKGEVKDEEELRELNRKLIDTAKKFDKIPVATGDVHFLDKHEA
                     VLRKVLKYSQGFKVDEEETYLHFRTTDEMLEEFSYLGEELAYEVVVENSNLIADMVED
                     IKPIPDDTYPPIIEGSDVELREMCYKKAKRIYGDPIPEIVQKRLDRELNSIISNGYAV
                     MYIIAQKLVAKSLSDGYLVGSRGSVGSSFAATMSDITEVNPLPAHYICENEDCKYSFF
                     YEIGEWGSGVDLPDKDCPKCGRKLAKNGHDIPFEVFLGFEGDKEPDIDLNFSGNYQPT
                     IHKYTEELFGEGYVYRAGTIGTVAEKTAFGYARKYVEENNISVPNAEVLRLSNGCTGV
                     KRTSGQHPGGVMVIPDYKDVYDFTPIQYPANDTSCGVITTHFDYHSISGRILKLDILG
                     HDGPTIIRMLEDMTGINITEIPLDDKETMSLFTSTEALGVTPEEINCPIGCLAVPEFG
                     TKFVRQMLLDTKPTTFAELVRISGLSHGTDVWVNNAQDLVREDIVGLKDVISTRDDIM
                     NYLIFKGLPPKMSFTIMESVRKGKGLKPEHIEEMKKNNVPEWYIGSCKKIKYMFPKAH
                     AVAYVMTSFRIAYCKVHYPEAFYATYFTTKVEDFDADLIVKGLDAIKSKINEIESLGN
                     DATTKEKGMLTVLEVALEMYARGIKLLPVDIYKSDATEFIVVGEKTLLPPMAAIQGLG
                     GNAAINIQNERKNGEFISKEDLRKRTKISKTVIETLTNHGSLENMSERNQLSLF"
     gene            8382..8843
                     /locus_tag="KR250_003201"
     CDS             8382..8843
                     /locus_tag="KR250_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HBH0069095.1"
                     /translation="MKKNLEATIEEIVTKITDEHGFEMVDVEYVKEAGEYYLRVYIDK
                     EEGISLNECELVSRELSPILDEKDPIKENYFLEVSSPGLDRALKKDRDFVRYQGRDVD
                     LKLYKPLNGCKQFEGELVGLTEDNNIKIIANGKEMEFNRKDVAIVRLAIKF"
     gene            8873..10027
                     /gene="nusA"
                     /locus_tag="KR250_003202"
     CDS             8873..10027
                     /gene="nusA"
                     /locus_tag="KR250_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HBH0069096.1"
                     /translation="MNHEFMEALDELVVDRGIDKEILIDTIEQALLTAYKKNFGSAQN
                     VRVEFDRDKGDIKVFSQRVVVDESDLYDTFLEIELADAREINPNYELGDIIEHEVTPK
                     NFGRIAAQTAKQVVVQRIREAEREIVYNEFIEKENEIVTGEVARANKNVVYVNLGRIE
                     GIMTQSEQIPFENYKAGQKIKFYILEVKKTNKGPQIVVSRSHPGLVKRLFEEEVPEIY
                     EGIVQIKSVAREAGSRTKMAVRSIDEKIDPIGACVGPKGLRVKNIVDELGDEKIDIIK
                     YSEDPAEFISAALSPSKVVRVDIDEDEKSALVIVPDYQLSLAIGKEGQNARLAAKLTN
                     WKIDIKSESQAEQEMLNLENSKEIEAEDVEAEFIDQEELDTSEDNFSEEE"
     gene            10034..10315
                     /locus_tag="KR250_003203"
     CDS             10034..10315
                     /locus_tag="KR250_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HBH0069097.1"
                     /translation="MILKKVKKVPQRKCIACQDRDSKKELIRIVKNKEGKIFLDPTGR
                     ANGRGAYVCKSSECLKKAIKSKALNKAFKIDVPDEVYDNLLMELEEYEK"
     gene            10305..10616
                     /locus_tag="KR250_003204"
     CDS             10305..10616
                     /locus_tag="KR250_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HBH0069098.1"
                     /translation="MKNNKEKVLSFLGLATRAGKIVSGDDSTLLDLKKGKVNLILIAE
                     DASNNTKKLFKDKSTFRNIPYLFFSTKEEIGFAIGKSPRAVVGIKDENFSKKIIELIE
                     I"
     gene            10638..12578
                     /gene="infB"
                     /locus_tag="KR250_003205"
     CDS             10638..12578
                     /gene="infB"
                     /locus_tag="KR250_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HBH0069099.1"
                     /translation="MSKTRVYQIAEELNISNEELINKLAELDINVTDKDSVLEGEELE
                     LALEMLGEDLSQENGNVIEIDGKLTVQVLATKLDKSPSEIIMKLMKMGTMATINQEIS
                     FEIAALAAKDYGFELTVAESDDTEALEIEALMEIEEDKEEDLKPRPPVVTVMGHVDHG
                     KTSLLDAIRKTDVISGEAGGITQHIGASEVKINGHKIVFLDTPGHEAFTSMRARGAQV
                     TDIAILVVAADDGIMPQTVEAINHAKAAGVPLIVAINKIDKPGANPDKVKQELADQGL
                     LVEDWGGEVIAVPVSAKKKEGIDTLLEMVLLVAEMEELRANPNKRAVGTVIEAELDKG
                     RGPVATVLVQGGTLTVGDPIVAGVACGKVRAMINAKGKRVKTAGPSTAVEILGLSEVP
                     QGGDQFVEVPTDKIARSVAARRQQIVRDEMLKSTQRLSLDALFSQMSEGSIKDLNIVI
                     KADVQGSVQAVKQSLEKLSNEEVQVKVIHGGVGAVTESDILLAAASNAIIIGFNVRPV
                     PGAESLGEKENVDIRTYTIIYKAIEDIQAAMTGMLDPEYVDEETGKAEIREIYKISGV
                     GTVAGCYVTNGKIFRNCKVRLVRDSIIIHEGELAALKRFKDDVKEVNSGYECGMSFVN
                     YNDIKEGDIVEAYITKEVERKL"
     gene            12621..13001
                     /gene="rbfA"
                     /locus_tag="KR250_003206"
     CDS             12621..13001
                     /gene="rbfA"
                     /locus_tag="KR250_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HBH0069100.1"
                     /translation="MASYNRTRRIAEEIRKVVSTMLINGVKDPRITSMVSVTDVEVTN
                     DLRYAYVYVSILGGDEESTLTGLKSAGGYIRREVGKNIKLRYIPEIVFKLDDSIEKGM
                     YMDSLIKRVNEKNAQQNEDENYDE"
     gene            12982..13659
                     /locus_tag="KR250_003207"
                     /pseudo
     CDS             12982..13659
                     /locus_tag="KR250_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438308.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
BASE COUNT         5489 a         1564 c         2584 g         4114 t
ORIGIN      
        1 tttgtagata tatcctaatt atgtcaaaaa ataaaaaaat ttttatttat tagagttttt
       61 ggaaataata ataacagata ttataattaa ttaacagttt ttgtaagtat ttgacaatag
      121 agagggtgta acaaatggga ataatagagt cagcatcaaa attggctgag atggttcatt
      181 tattggctgt tgaaaagggg ataactgata tagaagcatg ggatgaagct gtgaaagaat
      241 acagcaaaat ttatgaagaa agaagaaatg aatagaatga taaaaaatga aaacttgatg
      301 ataaaaaata actttattta aagaaattca atttttatag aagtattaaa aataatataa
      361 ataaaaagat ggtatttcaa atgtaagtaa aaaatcattt tgggatacca tccttattat
      421 ttattttaat aatatataag taataaatct tacttatcca tattttttat gtaacctctt
      481 ttttctaact tttttatatg ctccttacgt atttcatcaa gattaatacc atattgttct
      541 tctagcttaa acatcatagt aacacatgtc tgcgctacat ccattagttc cttagcagtt
      601 ttctttataa tatcaacttc atctaaatca ttattttctc cacttagatt tctgaattta
      661 cctatacatt cagctaattc accttgttct tcacccattt tgataaatgt agattctagt
      721 gttgggtcta aattattaag ctctggtaaa gatatagttt ttagtttcat aaaagctcct
      781 ttcaagttat tcgtatttat actaaaacat cataacaaaa ataatactaa aaaaaaactt
      841 taaattcaac ataaaaactt acatatataa taaaaatcat gaaataaatc tatatttgtc
      901 ttaatacagc ctcatataaa acttctccaa taggtttatt tattacaaga gaagctgact
      961 tatcatagac tgtatttgct ttattaataa gagcaatata atcacctttg taataatcta
     1021 taaaacttgc tgctgggtac acagctaatg atgtacctcc tataatcaat aaatcagcat
     1081 tacttatagc agaaattgtt ttagtaatga catcactgtc aagagcttcc tcataaagta
     1141 ctacatctgg ttttactata gaaccacagt tgtcacagta aggaatatta cctcccagat
     1201 ttaacataga ttctaagtca aaaaacttac cacatttagt acaataattt ctatgtacag
     1261 aaccatgaag ttctagcaca tttttacttc cagccatttg atgaagtcca tcaatatttt
     1321 gagttatgac agctttaagc ttacccattt cttcgagttt tgctaaagct atatgagcat
     1381 tattgggttt tgcattagga tatattaatt tatccttata aaattcaaaa aattcttctg
     1441 gatatctaac aaaaaaagta tgtgaaacta attgttcagc agtaaattgt ttattgagtt
     1501 tttggctaaa taatccagta gaacttctaa agtctggaat attagattca gttgaaacac
     1561 ctgctccacc aaaaaataca atattattgt gattagctat taagtctttt aagctatttg
     1621 catccatagt tacacctcca aattattata tataaattgt actacatatt aggtcgttta
     1681 tgttttatta atttaaataa atatatagtt taataagtta aataaaggga atataaataa
     1741 aatgtataca gagtataaat agacaatatt tactaaaata acaagttgtc gtataattat
     1801 attaaaattg ttttagtgta taagaagggc gggggaagta cgtgaagaaa aaagcagctt
     1861 tagcaacatt ggctatgtta cccttaggtg tggtaaatgc acatgctgat ggagatatag
     1921 gtatagtgac tataaattat ttaaatgtaa gaaatgaacc aactgccgaa agtagcatag
     1981 cctttgttgc taaaaaagat gataaagttt tgattaaaga ctcttctaat ggatggtata
     2041 aaataaaagc tgaatctgga caagaaggtt gggcttcatc aaaatatata gcaaaatcaa
     2101 atagtgactc tttaagaacg tctacaaata aagaaaaaca agttatttca aatagtttaa
     2161 atatgagaaa tggagcaggt acaagttaca gagtaataac tgtactaaaa aaaggtcaaa
     2221 aagtagaagt aatatcagag agtaatggat ggtctaaaat caaatatgat ggaagactag
     2281 gatatgtatc tagctcttat ttaggagacg tttcaaactc aactaataaa tcaaaaacta
     2341 aacaagtgaa tacaacttca ctaaatgtaa ggagtggacc aaatacaagt tatggtttgt
     2401 taggcaagtt gccaaaagga agtaaagtag aagtaatatc agaaagtaat ggatggtcca
     2461 aaataaagta taatggaaaa gatgcctatg tatctagtat gtacttatcg gatgtaagtc
     2521 aaagtaattc agacaattct agtcaaagca atgataaaaa gaatactgat aaggttgtaa
     2581 atacagcttc tttaaatgta agaagtggac caggttctac atatagtaag ttgggaaaag
     2641 tttataaagg aagtaaagta actgtactat cagaaagtag tggatgggct aagattaatt
     2701 ttaacaataa agaagcattt gtagtaggca attatttatc tacctcagca gatacttcaa
     2761 ataataactc aaatagtaac tctgataaca gttctaatag taatggtaat aattcttcat
     2821 catcaggcca agtaaatggt atgtcaggta taagtggggc taaaattgat tataaatctt
     2881 tgagttatac tttagaatct catataagta aacaagttga gaaagcagca tcaggaggaa
     2941 atgtgatagc tccaagtaat aggaaaagta ctccaagtcc tgagtttagt actttttcag
     3001 ctcaaagaac aagctcattt gtaaatgcaa gttcaagtga tatagaatat tatttaaatc
     3061 ctaagaattt tacaaatact actaaaggta tgatgcagtt tttaaagatt aacagttata
     3121 gagatggtat ttcagaatca agtcttaact cgtatcttaa tggtttatct tctagtgtat
     3181 ttaaaaacca gggagcagcc tttataaatg ctgccaagaa gtataatata gatgttgtat
     3241 atctagtatc tcatgctatg tgggaaactg cttatggtaa gtctacactt gcccaaggtc
     3301 aaacattaac atcttataag ggacaacctc ttagtaagcc agttaaagta tacaattttt
     3361 ttggtatagg agcaatagat aaaagtgcta atgtttcagg tgcagaagca gcgtattcca
     3421 atggctggac tagtgtagaa gctacaatag atggctctgc aaagtggata tcacaaaatt
     3481 acgtaaatag ttctaagtac aatcagaata ctatctacaa gatgaagtgg aattatgatt
     3541 atacatggca tcagtatgca actgatgtaa actgggctaa tggaatttct ggaattatgg
     3601 aaaatttaat tggtctttat ggaggaggaa gtagtttggt ttttgaagtt cctcaatata
     3661 agtaatacat aaataaaaaa tatagactat gggtagtttt cattttattt aaattatcta
     3721 tagtcttttt gtatataatt tataatctgt aaatttaatg ttaacaaaac ttttatgtta
     3781 taatattaat tctaagtaaa tataagtgag taataggtag aaatagataa aaagtggtaa
     3841 taatatatat aaagataatt ttgagaaagg aggatatata tggaaagtat taaggaatat
     3901 ttggataagt tagaaattaa taatagtggt cttggaaagc aacttaaaga agtttatata
     3961 aatagagtag tttattttaa ggaagataag atagtttatt tttatctaac ttctaaagat
     4021 attgtttctc atgaactttt agataaattt aaagaagaat tgatgtataa actcgattat
     4081 tttaaagata tgaggatgaa gataagattt actggtcttg aaagaaaatc caataaagat
     4141 gttataaaaa aatattggaa taatatattg tatatattaa agtatctttg tccatctatt
     4201 gcagggtggt ataaacaagt agaatttctt tgtttagaag aagaattaaa aataaaactt
     4261 ccaaaaggta tattttatga gcgattaatg aaaaaaaatg taacttatgt acttaaaacc
     4321 gtgcttagcg aagagttggg attagattta aatataacaa tagaaaaagc tgttgatgaa
     4381 aaggtaaata aagaaagact cataagaatc aatgatagag aaatggaaga aaaaattagg
     4441 gctttagaaa ttggaaaagt taataactgt gaagaaaatg aagaggaaag ctatgtcatt
     4501 aagtctgatg tagatgaaaa tttaatctat ggagataatg caaatgctat gattgaaaat
     4561 atagttgaat taaatgcatc ttctggaaca gttgcagttg ttggagatat atttgatgtt
     4621 gacactaaag aacttaaaaa cggaaaaata cttatgatag cttcaattac agattatact
     4681 agctcaataa gctgtaaact ttttttaaca gatacaaata aggatggagt acttgaaaat
     4741 gttaaaaaag gagcatactt aaaaatcaag ggtgacatag tttatgatac atatcaaaga
     4801 gaaattagta tgatgattag tgggataaga aaagaaacta gaatagagag agaagataaa
     4861 tcagaggaaa aaagagtaga actccatgca catacacaaa tgtcttctat ggatgctatt
     4921 tgttctgtta aaaagcttgt agagcgtgca gccaaatggg gtcattctgc tattgctata
     4981 acagaccatg gtgttgtaca aggatttcca gatgctatga atgcaggtaa gtcaaataat
     5041 ataaaaatac tttatggagt agaagggtac ctggttgaag atgattctct cattatagag
     5101 gatgcaaatg ataaagaact ttcgcagaca tttgttgtat ttgacataga gacaactggc
     5161 ttttctaata caaatgataa aataactgag attggtgctg taaaaataga aaactttaag
     5221 attgtagata gatttagtga gttaataaat cctgaaaaag atatttctta taagatacag
     5281 gaattgacag gaataactaa tgaattaatt aaagacaagc caaccattga agaagttctt
     5341 cctaagttta tggaatttgt tggagatagt gtattagtag cacataatgc agagtttgat
     5401 actggattta tatcgcaaaa atgtagggaa caaggcttgt tatataataa caaaaaagta
     5461 gatacactta tgttagcaag agtaatgttg ccaaacttaa aaagacatag attaaatgtc
     5521 gttgcaaaag aacttggtat tcctctttta aatcaccata gagcagtgga tgatgctgaa
     5581 gctacagcac ttatatttaa taagtttttg cagatgctta cagaaaaagg tgcaaaaact
     5641 ctaagtgatg taaacaatat acttggaaag attgattaca caaagcttag cacaaatcat
     5701 ataaccttga tagcaaaaaa ttctataggt ataaaaaatt tatataaaat aatttcagat
     5761 gcacatgtaa atcatttttt tagagcacca agaatcctta gaagtgtatt aaatgaatat
     5821 aaagaaggat taataattgg ttcggcttgt gaagctgggg tggtttttca agctgtaaag
     5881 aaaaacgtgt ctgatgagga aatgaaaaaa ataatagatt tatacgatta tattgaagta
     5941 atgcctatag ataataatag atttatgatt gataaaggag aagtaaagga cgaagaagag
     6001 ttaagggagt tgaataggaa actaattgat acagccaaaa aatttgacaa aattccagta
     6061 gctactgggg atgttcattt tttagacaaa catgaagctg tcttaagaaa agttcttaaa
     6121 tattctcaag gttttaaagt agatgaagaa gaaacttatt tacattttag aacaacagat
     6181 gaaatgttgg aagagtttag ttatcttgga gaagaattgg catatgaagt tgtagttgaa
     6241 aatagtaatc tgattgccga tatggttgaa gatataaaac ctataccaga tgatacttat
     6301 cctccaatta tagagggctc agatgtggag ttaagagaaa tgtgttacaa aaaagccaaa
     6361 cgaatctatg gagacccaat accagaaata gttcaaaaaa gactggatag agaattaaat
     6421 tcaataataa gcaatgggta tgcagttatg tatattatag ctcaaaaact ggttgcaaaa
     6481 tctctttcag atggatattt agttggttca agaggctcag ttggctcatc ttttgcagct
     6541 actatgagtg atataacaga agttaatcct cttccagcac attatatttg tgaaaacgaa
     6601 gactgtaaat attcattctt ttatgaaata ggtgaatggg gctcaggtgt tgatttacca
     6661 gacaaggatt gtccaaagtg tggaagaaaa cttgctaaaa acggtcatga tattccattt
     6721 gaggttttct taggttttga aggtgataag gaaccagata tagacttaaa tttctcagga
     6781 aattatcagc caacaattca taaatacaca gaagaactat ttggagaggg gtatgtatat
     6841 agggctggga ctataggtac tgtagcagaa aaaacagctt ttggatacgc aagaaaatat
     6901 gtggaagaaa ataatataag tgtaccaaat gcagaagtac ttaggctttc aaatggatgt
     6961 actggagtaa aaagaacctc aggacaacat cctggtggag ttatggttat accagactat
     7021 aaagatgtat acgattttac acctatacag tatccagcaa atgatacttc ttgtggtgtt
     7081 ataactactc actttgatta tcattcaata agtggtagaa tacttaaact tgatatactt
     7141 ggacacgacg gtcctaccat tataaggatg cttgaagata tgactggaat aaatataaca
     7201 gaaattcctt tagatgataa agagactatg tctctattta catctacaga agcattaggt
     7261 gttacaccag aagaaataaa ctgtcctata ggttgccttg ctgtaccaga gtttggaact
     7321 aagtttgtac gacaaatgtt acttgacaca aaacctacta catttgcaga acttgtacgt
     7381 atatctggtc tctctcatgg gacagatgtt tgggtaaata atgcacaaga cttagttaga
     7441 gaagatatag ttggattaaa agatgttata tctacacgag atgacataat gaactattta
     7501 atatttaaag gattacctcc caaaatgagc tttacaataa tggagagtgt aagaaaaggt
     7561 aaaggtttaa aaccagaaca cattgaagaa atgaaaaaaa ataatgttcc agaatggtat
     7621 ataggctcat gtaaaaagat taagtacatg ttcccaaaag cacatgctgt tgcttatgta
     7681 atgacatcat ttagaattgc atattgtaaa gttcactatc cagaagcatt ttatgcgact
     7741 tattttacga caaaagttga ggattttgat gcagatttaa ttgttaaagg attagatgct
     7801 ataaaatcaa aaataaatga aattgagtca ctgggaaatg atgcaaccac aaaagaaaaa
     7861 ggtatgttga ccgttttaga agttgctctt gaaatgtatg ctagaggaat aaaattatta
     7921 ccagttgata tatataaatc agatgcaact gagtttatag ttgtaggaga aaaaacatta
     7981 cttccaccaa tggcagcaat acaaggtctt ggaggaaatg cggctataaa catccaaaat
     8041 gaaagaaaaa atggtgaatt tatatcaaaa gaagatttga gaaagagaac aaaaatatca
     8101 aaaacggtaa tagaaactct tactaatcat ggttcacttg agaatatgag tgagagaaat
     8161 caattgtcat tgttttaagt tagcccaaaa agttgcaaat aaaagaattt tatgatatca
     8221 tagtatagaa aatagtatac tttgcagaaa ctaagagtga gacagctgtt ctcactcttt
     8281 tattttaaat atattattaa aaagctggtt tatttttatt aaatatattt tatttaagat
     8341 tatataaact ggtcatttac aattgaatag gaggaacttt aatgaaaaaa aatttagaag
     8401 ctacaattga ggagattgta actaaaataa cggatgaaca tggatttgaa atggttgatg
     8461 ttgaatatgt aaaagaagca ggagaatact acttaagagt atacatagat aaagaagaag
     8521 gaatatcttt aaatgaatgt gaattagtta gtagagaatt aagtccaata ctagatgaaa
     8581 aagaccctat aaaagaaaat tactttttag aggtatcttc tccagggctt gatagagcat
     8641 taaaaaaaga cagagatttt gttagatatc aaggtagaga tgtagattta aaattatata
     8701 aaccattaaa tggatgtaag caatttgaag gtgaacttgt aggattaaca gaagataata
     8761 atataaaaat cattgcaaat ggtaaggaaa tggaatttaa tagaaaagat gttgctattg
     8821 tcagattagc aataaagttt tagtggtata ttaaataggg aggatataaa aaatgaatca
     8881 tgaatttatg gaagcattag atgaattagt agttgacaga ggtatagata aagaaatact
     8941 tatagatact atagaacaag ctcttttaac tgcatacaag aaaaactttg gttcagcaca
     9001 aaatgttaga gttgaatttg atagagacaa gggagatata aaagtatttt ctcaaagagt
     9061 tgttgttgat gaatcagatt tatatgatac tttcttagaa atagagttag cagatgctag
     9121 agagataaat cctaattatg aattaggtga tattatagaa catgaagtta cacctaaaaa
     9181 ctttggaaga attgcagctc aaacagcaaa gcaagtagtt gttcaaagaa taagggaagc
     9241 tgagagagaa atagtgtata atgaatttat agaaaaagaa aatgaaatag ttacaggaga
     9301 ggttgctaga gcaaataaaa atgttgttta tgtaaatctt ggaagaatag aaggtataat
     9361 gactcaaagt gaacaaatac catttgagaa ttataaagca ggtcaaaaaa taaaattcta
     9421 tatacttgaa gttaaaaaga ctaacaaagg acctcaaata gttgtatcaa gaagtcatcc
     9481 gggtctagta aagagattgt ttgaagaaga agttcctgaa atatatgaag gaatagttca
     9541 aataaaatct gtagcaagag aagcagggtc aagaactaaa atggcagtaa gatctataga
     9601 tgaaaaaata gacccaattg gagcttgtgt tggaccaaaa ggacttagag taaaaaatat
     9661 agtggatgaa cttggcgatg agaaaataga tataataaaa tatagtgaag acccagcaga
     9721 gtttatatca gcagcactta gcccatcaaa ggttgttaga gtagatatag atgaagatga
     9781 aaagtctgcc ttagtaatcg ttccagatta tcagttatcg cttgctatag gtaaagaagg
     9841 tcaaaatgca agactagcag caaaattgac taactggaaa atagatatta agagcgaatc
     9901 tcaagcagaa caagaaatgt taaatcttga aaattccaaa gaaatagaag ctgaggatgt
     9961 tgaagctgaa tttatcgacc aagaagagtt agatacttca gaggataatt ttagtgaaga
    10021 agagtaagga gggatgattt tgaaaaaagt aaaaaaagtg cctcaaagaa aatgcatagc
    10081 atgtcaagat agagactcta aaaaagagct tataagaata gttaaaaata aagaaggaaa
    10141 aatattttta gacccaacag gaagagccaa tggaagagga gcatatgtat gtaaaagctc
    10201 tgaatgtcta aaaaaagcta taaagagtaa agcattaaat aaagctttta aaatagatgt
    10261 tcctgatgaa gtctatgata atctacttat ggagttagaa gaatatgaaa aataataaag
    10321 aaaaagtact ttcgttctta gggctcgcaa ctcgtgcagg gaaaatagtg tctggtgatg
    10381 attcaacttt gcttgacctt aaaaaaggaa aagtaaattt gattttaatc gcagaagatg
    10441 catcaaataa tactaaaaaa ctttttaaag ataaatcaac ttttagaaat attccatatc
    10501 tatttttttc aacaaaagag gaaataggat ttgcaatagg aaaatctcca agagcagttg
    10561 taggaataaa agatgaaaat ttttctaaaa agataattga actaatagaa atttaaaata
    10621 aaataggggg tgatcttgtg tcaaaaacaa gagtatacca aatagcagaa gaattaaata
    10681 tatcaaatga agagcttata aataagttag ctgaattaga tataaatgta actgacaaag
    10741 attcagtatt agaaggtgaa gagttagaat tagcacttga aatgttaggt gaagatttat
    10801 ctcaagaaaa tggaaatgtt atagaaatag atggaaagtt gacagtacaa gtattagcaa
    10861 cgaaattaga taaatctcca tcagaaataa ttatgaagct tatgaaaatg ggaactatgg
    10921 caacaataaa tcaagagata agttttgaga ttgctgcttt ggctgccaaa gactatggat
    10981 ttgaattaac agtagctgaa agtgatgata cagaagcttt agaaatagaa gctttaatgg
    11041 aaattgaaga agacaaagaa gaggatttaa aacctagacc accagttgtt acagttatgg
    11101 gacatgttga ccatggtaag acatctttac tagatgcaat aagaaaaact gatgtgatct
    11161 caggtgaagc tggaggaata actcagcata taggtgcttc agaagttaaa atcaatggac
    11221 acaaaatagt atttttagat actccaggac atgaggcttt cacatcaatg agagcaagag
    11281 gagcacaagt tacagatata gcaatattag tagttgcagc agatgatggt ataatgcctc
    11341 aaacagttga agctataaac catgctaaag cagcaggtgt acctttaata gttgctatta
    11401 ataaaataga taaaccaggt gcaaacccag ataaggttaa acaggaatta gcagaccaag
    11461 gattattagt tgaagactgg ggtggagaag taatagcagt tccagtatca gctaagaaaa
    11521 aagaaggaat agatacactt ttagaaatgg tattattggt tgctgaaatg gaagagttaa
    11581 gagctaatcc aaataaaaga gcagtaggaa ctgttataga agctgagctt gataaaggaa
    11641 gagggccagt tgcaacagta cttgttcaag gtggtacttt aacagtaggt gacccaatcg
    11701 ttgcaggtgt tgcatgtggt aaagttagag caatgataaa tgcaaaaggt aagagagtaa
    11761 aaacagcagg accatctaca gcagtcgaga tattaggtct atcagaagtt ccacaaggag
    11821 gagaccaatt tgtcgaagtt ccaactgata agattgctag aagtgtagca gcaagaagac
    11881 aacaaatagt tagagatgaa atgttaaaat caactcaaag attatcacta gatgctctat
    11941 ttagtcaaat gagtgaaggt tctataaaag accttaatat agttataaaa gcagatgtac
    12001 aaggttcagt acaggcagtt aaacaatctt tagaaaaatt atcaaatgaa gaagttcaag
    12061 ttaaagtaat acatggtggt gttggagctg taacagaatc agacatatta cttgcagcag
    12121 cttctaacgc aataataata ggattcaatg taagaccagt tccaggtgca gaatctctag
    12181 gagaaaaaga aaatgttgat ataagaacat atactataat atacaaggca atagaagata
    12241 tacaagcggc tatgactggt atgttagacc ctgaatatgt tgatgaagaa acaggaaaag
    12301 ctgaaataag agaaatatat aaaatatctg gtgttggaac agtagctgga tgttatgtga
    12361 ctaatggtaa gatatttaga aattgtaaag taagattagt tagagatagc ataataatac
    12421 atgaaggtga gcttgcagca cttaagagat ttaaagatga tgtaaaagaa gttaattctg
    12481 gatacgaatg tggtatgagt tttgtaaatt ataacgacat aaaagaaggc gatatagtag
    12541 aagcttatat aactaaagaa gtagaaagaa aattataaat tcgtatcaaa gaaaatcagt
    12601 tagtaaagaa ggtcgatggt atggcatcat ataatagaac aagaaggata gcagaagaaa
    12661 taagaaaagt cgtaagtact atgcttataa atggagtaaa agaccctaga ataacatcta
    12721 tggtgagtgt aactgatgta gaagttacaa atgacctaag atatgcttat gtatatgtaa
    12781 gtattctagg cggagatgaa gaatccacac taacaggtct taaaagtgct ggaggatata
    12841 taagaagaga agtaggaaaa aatataaaac ttagatatat tcctgagata gtatttaaac
    12901 ttgatgattc aatagaaaaa ggaatgtata tggatagcct tataaaaaga gttaatgaaa
    12961 aaaatgctca acaaaatgag gatgaaaatt atgatgaata atatggatag tattataaac
    13021 tatatttatg agggtgacta ttttatagtt acctctcata tcagtccaga tggagacaat
    13081 attggttcta cactttcaat gtattataca ttgaagaatt taggaaaaaa tgtatattat
    13141 gtacttgatg atgaagctcc attaaattta aggtttttgg ttgaaggtgt aaaaatctat
    13201 aagtctaatg aatttaatat gaagaactat tctctaattg cacttgattg tggggataag
    13261 atgagaatat gtgtgagtga tgaaatcaaa aataatgcat caaaattaat ctgtattgac
    13321 catcatgcta gtaatgattc atatggagat ttaaattatg tagatattga tgcatcttct
    13381 acatgtgaat tagtttataa tttattagtt agatttcagc aaataaaaga tattaatatt
    13441 ataaatgaag atatagcaac atgtttatat acaggtcttg taactgatac tggaaacttt
    13501 tcgtattcaa atgtacatgc aagtagcttt gaaatggcaa aaaatttact tgttttaggt
    13561 gcacaaaaaa atactataat acaaaatata tatcaaagca attcttctgg ttattacaaa
    13621 ttattaggag aagctctaaa gggtttggag atatttgatt aaaaaatagt tacgcacact
    13681 cgtgacttta gtcatgagtt agtagctaaa atagtcagca tatagggaaa cttatatgta
    13741 gagataggat a
//
LOCUS       DAEPIN010000089        13508 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.089, whole genome shotgun sequence.
ACCESSION   DAEPIN010000089 DAEPIN010000000
VERSION     DAEPIN010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13508
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.089"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            960..2318
                     /locus_tag="KR250_003208"
     CDS             960..2318
                     /locus_tag="KR250_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine/pyrimidine permease"
                     /protein_id="HBH0069101.1"
                     /translation="MKAKSTSKFELDGIPPLKEAIPLGLQHLMAMVIGSCLPAILIAN
                     AAGLNHKMSTLLIQAALVFAGLSTLLQLYPIPLFKGFKLGGGIPVIMGATAMFIGAGV
                     SVASKYGLPVLFGSQIIAGIVIIIIGFGLKKIRFIFTPAVSGTIVACMGLGLFPIAIK
                     NLAGGMGNETFGNPINFIVGISVSLMILGLNKYGKGLFKDASILVSIIFGYILSLILG
                     IVNFSSIQEFTLVALPKPLAFGLDIRLEVVVMFSIIYLVEIADIMGACTLSAVGGLNR
                     QVTDEELSSAVLGNGVLSTIGALFSATPMGMFGQNSAIVSNTKVVSKFVLAIGGIGLF
                     LAGISPLLANLIRTIPPCVVGGATLVIFSTLTTSGLRLVSMDGFNQENSMILGLSMAS
                     GIGFMVAPQVLEKFPKFIETLLADSSVVSGAMVAIIIQALYMVKFPGNKSDKVEDKNK
                     SL"
     gene            2425..3219
                     /locus_tag="KR250_003209"
     CDS             2425..3219
                     /locus_tag="KR250_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HBH0069102.1"
                     /translation="MVTIKEYSVPQSLEEAYKILISKKNNVILGGCGFIKLSNKNIGT
                     AIDLKDINLNYIKEDKKNILIGADTSLRSLEIDKTIKNYCSGILSSAVSNIVGVQFRQ
                     GARIGASVFSKYGFSDLIPALLVVGAKVRLYNKGILELSEFLDSELSRDILVEVILPK
                     KDAIAVFDSIRKCTGDFSVLNSAMLKENDTYKIAIGARPQKARLALEASNILNKEKDI
                     DKASIVASKELTYGSNMRASKEYRKDMASALVKRMYNAIEEGMYND"
     gene            3212..5518
                     /locus_tag="KR250_003210"
     CDS             3212..5518
                     /locus_tag="KR250_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBH0069103.1"
                     /translation="MINKGIRKIDSTAIVTGKPLYTEDLIMHKDVLTLKLLRSPHAHA
                     KIKNIDTSKAEKIPGVVGIYTYKDVPQVRYCVPGQTEPEASPYDRVLLEDTVRFIGDE
                     VALVAAEDEKTALKALKMIKVDYEVLKPVFDAREAEGNEVVIHPEKDIVEPYPFGLDA
                     KNNIVSKEFFEFGDVEAKLKESDFVVESSYKTQSQSHCMMETHRSYSYKDINDRIVVV
                     SSLQSPHNVKRILRKVLGIPSSKIRVIKPRIGGGFGGKNIAATDIFTAFVTMKTGRPA
                     KFVFTRSETFVGSNSRHEMFFDVKVGADKEGNLKAIELKSLNNTGAYGGNGVSVSSES
                     GHNTLPIYNDIDAVRFNARTVYTNRLPAGALRGYGATQGIFAIDSAIDELAHKIGMDP
                     LEFRIKNIIDRYTNGKAAEDEILSCELRQCIEKGKKLINWDDKYPCKDIGNNKVRAVG
                     MAVANHGSGIPRVDMATVTIRMDEDGTYKLLSGLADLGQGADTIQAQIAAEALNTTVD
                     RISIYTGDSDLCPYDTGAFASCTTTVTGNATIKTANKLKDILLSVAESKLGFKKEDLE
                     LGKDRVFVKNDNSKFVLLEELGRESVGGIGFASDDVPVVLEATESFGMLKKIKPFIAG
                     FAEIELDKNTGEYKVINFACVPDCGTVINPTLARIQVEGGVMMGIGFAMYEDPIFDKD
                     GRLLTDSFLQYKVPTKKEVGNILVDFAENFDPGGPYGAKSLGEIVIHTPAPAIANAIF
                     NATGVRLRELPMTFEKVYMGMKNNNKQL"
     gene            5657..6934
                     /locus_tag="KR250_003211"
     CDS             5657..6934
                     /locus_tag="KR250_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBH0069104.1"
                     /translation="MSNRIKNIHYGTQRQQLKNLTKKEYQALREMCFLAKNLYNVGTY
                     NVRQHYFNQGEHLNYEANYHVCKENENYKLLNSNIAQQILKEVDSSFRSFFGLIKLAK
                     EGKYDFRGIKSPKYLDKEGFFSIIIGQIRIKEDGILNVPMSPAFKKLYGKVSIKVPQN
                     ILEKKIKEIRIIPKHKARFFEIQYCYEIPKSEEVSNQKNALAIDLGLENLCTCVTNLG
                     DSFIIDGKRLKSVNQWANKQNDKIEALKLKKNITAITRKQFKVWNKRNNQVKDYVNKT
                     CFYIIDYCVKNNIGNLIVGYNAKLEKSDNMERKIYRNFIRIPFGEIKGKLEYLCKIKN
                     INFIRQEESYTSKSDFFADDFLPDINVDNVKKYDFKGERITRGQYKSSVGIIINADVN
                     ASLNILKKSQVVNLSNLQNNPQILKQPQRVRIS"
     gene            7117..7839
                     /gene="yqeC"
                     /locus_tag="KR250_003212"
     CDS             7117..7839
                     /gene="yqeC"
                     /locus_tag="KR250_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium-dependent hydroxylase
                     accessory protein YqeC"
                     /protein_id="HBH0069105.1"
                     /translation="MELSNIIEKNEIITVVGAGGKTSFINYFANFYRDKLKVLLTTTT
                     KIYVPNDYDNIIITIDGTVIPSICHGITVCGSYINNENKLVSIDSSILDEIVDQFDLV
                     LIEGDGSKRKKLKGWNAKEPVVYHKTTKTIGILDITSFGMNINEENIHRVEIFKKIAN
                     LDTSSINSSSTVSIENLKNIVLNPNGLFKNYSGKRVLFINKVENEKYKNLAIKLVENI
                     KEYESEIEIFYGSVKQKFCVRY"
     gene            7946..8515
                     /gene="mocA"
                     /locus_tag="KR250_003213"
     CDS             7946..8515
                     /gene="mocA"
                     /locus_tag="KR250_003213"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HBH0069106.1"
                     /translation="MINAVIMASGFARRMGANKLLLNYKGCSIIEHVFREVSKIDFHQ
                     VVVVSQYEEVLLLCNKYGFEYIDNKNANIGQSESIKLGVLNSSKCDGYMFFVGDQPFI
                     DNLYIDKIINTFKLDRDFIVIPRYKDMCGNPVIFPYSKKEELLALKEDEKGKTILKNS
                     SKIKYVEVPKEMLFDIDTKMDYERIGGKL"
     gene            8512..9531
                     /locus_tag="KR250_003214"
     CDS             8512..9531
                     /locus_tag="KR250_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HBH0069107.1"
                     /translation="MKLIKTIDAVGQVLCHDITQIIPGEFKGRKFKKGHIVKEEDIPV
                     LLSLGKDNLYVWEKSEGMVHENEGALFLKELTAGENLDFSEIKEGKIDFIAACDGLLK
                     IDVDALFDLNCVGEIMMATLHNNFIVNKGLKVAGTRVIPLVIDEKKLEDAKKVVGNRK
                     IVNVVPFKPKKVGIVTTGNEVFYSRIVDKFGPVIEEKVKGFGCEVIGQTICPDDKEII
                     KSAIKEFISQGAELICCTGGMSVDPDDVTPTAIKETGADLVTYGSPILPGAMFLLAYY
                     GEVPIMGIPGCAMYHKTTVFDIVLSRVLIDEKLDKYDIARYGHGGLCMNCDVCTYPAC
                     NFAKI"
     gene            9869..11494
                     /locus_tag="KR250_003215"
     CDS             9869..11494
                     /locus_tag="KR250_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBH0069108.1"
                     /translation="MLQVTGVGLRFGDKELFKDVNLKFTKGNCYGIIGANGAGKSTFL
                     KILSGEIEPNTGSISITDKERMSVLKQDHFEYEEETVLNVVIRGHERLWNIMKEKDAL
                     YMKEDFNEEDGIKAAELEGEFAELDGWDAETNAEKILMGLGITKDMHYKQMKELVGGE
                     KVKVLLAQSLFGKPEILLMDEPTNHLDFKSINWLNNFIMDLEESIVIIVSHDRHFLNQ
                     ICTNIVDVDFGKIQMYVGNYDFWYESSQLALQLAKDQNKKTEEKIAQLKEFIARFSSN
                     ASKAKQATSRKKQLEKLEVEDIQPSRRRYPYVGFTPAREIGNEVLEVHNLTKTIDGVK
                     VLDNVSFRLDRDDKVVFMGDEIATTALFNIVMGELEPDSGEYKWGVTTSQDYLPKNHN
                     KFFDGVEYSLVDWLRQFSEEKSESFIRGFLGRMLFSGEEALKEAQVISGGEKVRCMLS
                     KLMLSNANVLVLDDPTNHLDLESITSVNKGLEKFPGVLLFTSHDHEFISTIANRIIEI
                     TPNGIMDRKMDFDEYLESKEIQDQLAKMYGKDK"
     gene            11693..12169
                     /locus_tag="KR250_003216"
     CDS             11693..12169
                     /locus_tag="KR250_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069109.1"
                     /translation="MVKKRLVAVVISLAMISVGNFSYAHSGGHNSSGDHHNKNKSELT
                     RYYCGGHSAHQHNKGFCPYASTKENSGVTISSVHNEKNHKNYFAEKGYNKGYEDGCKG
                     LYNLSVTEAKSDNLYEKEILKSSYEKGYLIGYNEYKAKKVDDVMKKMLDIPMLASI"
     gene            complement(12377..13489)
                     /locus_tag="KR250_003217"
     CDS             complement(12377..13489)
                     /locus_tag="KR250_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBH0069110.1"
                     /translation="MNAFIRKRNKNYVVYLEFEDEKTGKRKQKNMGTFDKKREASKRL
                     NEVKESLYNDGFLVPSEITVSEFLLDFLNKYSENISLATYNNYVSICKNYINPSIGKY
                     KIQDLHPVHIQNYIDKLSYKLNPQSIKIHINILKLAIKRAYRLKLIKENVMDSIEAPR
                     YKKFKNEIYDREQMIKLLDLAKNTEMELPINLAIGLGLRISEVLGLTWDNIDFEENTI
                     TVNKITSRINGSVILKEPKTESSVRKISSPKELMSLLKEYKIKQNKNLLKSSIRNNNN
                     LLFFNKKCEPIAEDVMSKKFKRFLEKNELPHIRFHDLRHSHVTLLINSKVPIKVISER
                     VGHSNISTTLSVYSHVLKEMDKEASDKISESLFNAN"
BASE COUNT         5277 a         1430 c         2426 g         4375 t
ORIGIN      
        1 atagaaactt gtatatttta tgttgcagat acaaatatag taaaggagag tacattggta
       61 caactgagat acatagctta ttaagttttt ctgtcttatg agaattatat tataaaatat
      121 tcataaatat tttgagttta aattaaataa ttattttatg cttagattaa ttgatattta
      181 attaatttaa taacaaaaac agtgaaagag taaataatat tatttacagg gggtaaagca
      241 aagtatgtta aataaattgt acttttggcc ccgtacatag agaaatctat gtaggacaca
      301 actttaaaac cagtaaatcc taaagccttg caactacaat aaaagataga aagaaacttt
      361 tatgaatgtg gtgaaaacca gaaacaatag tgaggatggt atatggtgag agcctaagta
      421 cttaaaaatg gacatttggt cgccaagcct agaaatagga aggtcaaacg actatctcga
      481 gagagagtac actataagcg attgatagtg gaaacgggtt gctcttagca ggtaatgctg
      541 aagatgaaga aatagtctac gctcatatga aagtatgagg tcactggatt tgaagtgaca
      601 tctataaagt agcgtttata ggtaaatgtt taaaacttga aacatgtaaa tacttatgaa
      661 aacttataac ttataatgat taagattgaa agatttaaat tgaattaaat ttactataaa
      721 aaattaaaaa ttcatagcta taatactctt atatttgtaa agcatggtat ataatgtata
      781 caaaattaaa gaatacgttt atttgttaat aattaaattt attgcatctg ttttttaaga
      841 ctaaaaactt aaatatatgt tggagtatag ttgatattta tgaacatcat taatttaata
      901 tattagattt ttaagtattt atataattca ataagtttta tgtaacggga gtttttatta
      961 tgaaagcaaa aagtacatca aaatttgaat tagacggaat accaccacta aaggaagcaa
     1021 tacctttagg gttacaacac ttaatggcaa tggtaatagg tagttgttta ccagcgatat
     1081 tgattgcaaa tgcagctgga cttaatcata agatgtcaac attattgatt caagccgcat
     1141 tagtttttgc aggattatca actttattac aattatatcc aatacctttg tttaaaggtt
     1201 ttaaattagg aggaggaata ccagttataa tgggagctac agctatgttt ataggagctg
     1261 gggtatctgt tgcaagtaag tatggactac cagtattatt tggctctcaa ataatagctg
     1321 gtatagtaat aattataatt ggttttggtc ttaaaaaaat acgatttata tttactccag
     1381 ctgtatcagg aacaatagtt gcatgtatgg gtcttggact ttttccaata gctataaaaa
     1441 acttagctgg gggtatgggc aatgagactt ttgggaatcc aattaatttt atagttggaa
     1501 tatctgtatc tcttatgata ttaggattaa ataagtatgg aaaaggatta tttaaagatg
     1561 catcaatatt agttagcata atttttggat acattttatc attaattttg ggtatagtaa
     1621 atttttcttc aattcaggag tttactttgg ttgcacttcc aaaaccactt gcttttgggc
     1681 tagatataag acttgaagtt gttgtaatgt tttcaataat atatttagtt gagatagctg
     1741 atataatggg tgcttgtaca ctttctgcgg taggagggct taatagacaa gtaacagatg
     1801 aagaactttc ttctgctgtt ttaggaaatg gagtactctc tacaataggt gcattatttt
     1861 ctgcaactcc aatggggatg tttggacaaa attctgcaat cgtttccaat acaaaggttg
     1921 taagtaaatt tgtactagct attggaggaa ttggattatt tttggcagga atatcgcctt
     1981 tattagctaa tttaataaga acaattcctc cctgtgtggt tggaggagca acactcgtta
     2041 tattttcaac tcttacaaca tctggtttaa gacttgttag tatggatgga tttaatcaag
     2101 aaaatagcat gatattaggt ctttctatgg cttctggtat tggattcatg gtagctcctc
     2161 aagttcttga aaaatttcca aaatttatag agacattact tgcagattca agtgtagttt
     2221 caggagcaat ggtagccata ataattcaag ctttatatat ggtaaaattt cctggaaata
     2281 aaagtgataa ggttgaggat aaaaataaaa gtttataatg taaaggttta atatatgtga
     2341 attaaatata ctacaattat aaatataaat aaaagattta ttaaaaactt ttgtgtttta
     2401 ataagttaat gatgggggga taagatggtt actataaagg aatattcagt tccacagagt
     2461 ttagaagaag catataaaat tttaatttct aaaaaaaata atgttatttt aggtggatgt
     2521 ggatttataa aattaagtaa taaaaatata ggaacagcga tagatttaaa agatataaat
     2581 ctaaattata taaaagagga taaaaaaaat attttgatag gtgcagatac ttcacttaga
     2641 agtttagaaa ttgataaaac tataaaaaat tactgtagtg gaatactttc aagcgctgtt
     2701 tctaatatag ttggtgttca atttagacaa ggggctagaa ttggtgcaag tgtattttca
     2761 aagtatggct tttctgactt aataccagcg cttttggttg ttggagcgaa ggtgagatta
     2821 tacaataaag gaatactaga gttaagtgag tttttggata gtgaattaag tagggatata
     2881 ttggtcgaag taatactacc aaaaaaagat gcaatagctg tttttgactc aataagaaaa
     2941 tgcacaggag atttttctgt attaaattca gcaatgctaa aagaaaatga tacttacaaa
     3001 atagcaattg gggcaagacc tcaaaaagct agattagcac ttgaagcgag taatatatta
     3061 aataaagaaa aagatataga taaggcatct attgttgcta gtaaggaact tacttatgga
     3121 agtaatatga gagctagtaa agagtacaga aaggatatgg caagtgcatt agttaaaagg
     3181 atgtataatg ctattgagga gggaatgtat aatgattaat aaggggataa gaaagataga
     3241 tagtacagca attgtcacag gaaaacctct atatactgaa gacctcatta tgcataaaga
     3301 tgttttgaca ttaaaactac ttagaagtcc acatgctcat gctaagataa agaatataga
     3361 tacatcaaaa gccgaaaaaa ttccaggggt tgtgggtatt tatacgtata aagatgtacc
     3421 acaggtcaga tattgtgtgc caggtcaaac agaaccagaa gcatcacctt atgatagagt
     3481 tttacttgaa gatacagtta gatttattgg agatgaagta gctttagtgg ctgctgaaga
     3541 tgagaaaacg gcacttaaag ccttaaaaat gataaaggta gattatgagg ttttaaagcc
     3601 tgtgtttgat gcaagagaag cagaaggaaa tgaagttgtt atacatccag aaaaagacat
     3661 agtagagcca tatccttttg gattggatgc aaaaaataat atagtatcaa aagaattttt
     3721 tgaatttgga gatgttgaag ctaagcttaa agaaagtgat tttgtggttg aatcttcata
     3781 caaaacccaa agtcaatcgc attgcatgat ggaaactcat aggtcatatt catataaaga
     3841 tataaatgat agaattgttg tagtttcatc attacaatca ccacacaatg taaagagaat
     3901 acttagaaaa gtattgggta taccatcttc aaaaataaga gtaattaaac ctagaatagg
     3961 tggtggattt ggaggaaaga atatagcagc aacagatata tttactgcat ttgttactat
     4021 gaaaactggt agaccagcaa aatttgtatt tacaagaagt gaaacttttg taggttctaa
     4081 tagtagacat gaaatgtttt ttgatgttaa agttggtgca gataaagaag gaaatttaaa
     4141 agcaatagag ctaaagtcat taaacaatac aggtgcttat ggtgggaatg gtgtctctgt
     4201 ttcttccgaa tctggacaca ataccttacc aatatataat gatattgatg ctgttagatt
     4261 taatgctaga acagtatata ctaataggtt accagcagga gcacttagag ggtatggtgc
     4321 tacacaggga atatttgcaa tagatagtgc tattgatgaa ttggcacata agattggaat
     4381 ggatccactt gaatttagaa ttaaaaatat aatagatagg tataccaatg gaaaagcagc
     4441 tgaagacgaa atattaagtt gtgagctgag acaatgtata gaaaagggca agaaattaat
     4501 taattgggat gataaatatc catgtaaaga tataggaaac aacaaagtaa gagcagtagg
     4561 tatggcagta gcaaatcatg gttctggtat accgcgtgta gatatggcaa cagtcactat
     4621 aagaatggat gaagatggca catataaact attatctggt cttgctgact taggtcaagg
     4681 ggctgataca attcaagctc aaattgctgc ggaagcttta aatactactg tagatagaat
     4741 ttctatttat acaggtgatt cagacttatg tccctatgat acaggagcat ttgcttcttg
     4801 tacgacaacg gttacaggaa atgcaacaat aaagacagca aataaattaa aagatatatt
     4861 actttcagtt gcagagtcaa agctaggatt taaaaaagaa gatttagagt tgggaaaaga
     4921 cagggttttt gttaaaaacg ataatagcaa gtttgttcta ttagaggaat tgggtagaga
     4981 atctgttgga ggaataggat ttgctagtga tgatgtacca gtagttttgg aagcaacaga
     5041 atcttttggc atgttgaaaa aaataaaacc atttatagca ggatttgcag agatagaatt
     5101 agataaaaat acaggcgaat ataaagtcat aaattttgca tgtgtacctg attgtggaac
     5161 agttataaat ccaactttag ctagaataca agtagaaggt ggtgtgatga tgggtatagg
     5221 gtttgcaatg tatgaagacc ctatatttga taaagatggt agattattaa cagatagttt
     5281 cctacagtat aaagttccta caaaaaaaga agttggaaat atattggtag attttgctga
     5341 gaatttcgac ccaggaggtc cttatggtgc taaatcatta ggagaaatag ttatacatac
     5401 accagcacca gctatagcaa atgcaatatt taatgctaca ggagttcgtt taagagagct
     5461 tcctatgact tttgaaaaag tttatatggg tatgaaaaat aataataaac aattataata
     5521 aataataatg tgtataaaag caagtaaatt taaattattt tgtaaaataa agtaattaaa
     5581 tggacattat atatggtgtt ttgtaaaaat accgtatata atgtctagta gagtatattc
     5641 taaaggaggt gagttcatgt caaacagaat aaaaaatata cactatggaa ctcagagaca
     5701 acaattaaaa aacttaacta aaaaagaata tcaagctcta cgagagatgt gcttcttagc
     5761 taagaactta tataatgttg gtacatataa tgtaagacag cattatttca atcaaggaga
     5821 acatcttaac tatgaagcta attatcatgt atgcaaagaa aatgagaact ataagcttct
     5881 caatagtaat atagcgcaac aaatattaaa agaagtagac agttcgttta ggtcattttt
     5941 tggattaatt aaattagcca aagaaggaaa atatgacttt agagggataa aatcacctaa
     6001 atatttagat aaagaaggat tttttagcat aataataggt caaataagaa tcaaagaaga
     6061 tgggatttta aatgtaccta tgtctccagc atttaaaaaa ctatatggga aagtaagtat
     6121 aaaagttcca caaaatatac tagagaaaaa aattaaagaa ataagaataa tacctaaaca
     6181 taaagctagg ttctttgaaa ttcagtattg ttatgaaata ccaaaatcag aggaagtttc
     6241 aaatcaaaaa aatgcactag ctatagactt gggtttagaa aatttatgta catgtgttac
     6301 caatttaggt gatagtttta tcatagatgg aaaaaggcta aaatctgtga atcagtgggc
     6361 aaacaaacag aatgataaaa tagaagcctt aaaattaaaa aagaatataa ctgcaataac
     6421 aagaaaacaa tttaaagttt ggaataagag aaataatcaa gttaaagact atgtaaataa
     6481 aacctgtttt tatataattg attattgtgt aaaaaataat attggaaatt taattgtagg
     6541 atataatgct aagttagaaa aaagtgataa tatggagaga aaaatatata gaaattttat
     6601 aagaatccca tttggagaga taaaaggaaa attagaatat ctgtgtaaaa taaaaaatat
     6661 caattttatt aggcaagaag aaagctatac tagtaaatca gatttttttg ctgatgattt
     6721 tctacctgat atcaatgtgg acaatgttaa aaaatatgac tttaaagggg aaagaataac
     6781 gcgtggacag tacaaatcca gtgttggaat cattataaat gcagatgtaa atgcgagtct
     6841 taatatacta aaaaaatcac aagtagttaa tttatctaat ttacagaata atcctcaaat
     6901 attaaagcaa ccacagagag taagaatatc ttaattatat ttaaaagata aactgatagc
     6961 gtaagcttac agcttagcgg cgaattggat ccgccttgtt gtttacaaca gaatctattt
     7021 actttcgagt ttatggaatt tcaaaaatca cttttactaa aaaggtctaa ctataataat
     7081 taagagatta gaaaactggt tataagaggt gaaagtgtgg aactatcaaa tataatagaa
     7141 aagaatgaaa taataactgt agttggagca ggaggaaaaa cttcgtttat aaactatttt
     7201 gcaaattttt atagagataa attgaaagtt ctccttacaa caacaactaa aatatatgta
     7261 ccaaacgatt atgacaatat aattattaca atagatggaa ctgttattcc atctatttgt
     7321 catggaataa cagtctgtgg cagttatata aataatgaaa ataaattagt aagtattgat
     7381 tcctctattt tggatgaaat tgtagaccaa tttgatttag ttttaataga gggagatggc
     7441 tcaaagagaa aaaaacttaa aggttggaat gctaaagaac cagtagtata tcataaaact
     7501 acaaaaacca tagggatatt agatataaca tcatttggaa tgaatattaa tgaagagaat
     7561 atacatagag tagaaatatt taaaaaaata gcaaatttag atacaagtag tattaatagt
     7621 agttctacag taagtattga aaacttaaaa aatatagtat taaatccaaa tggattattt
     7681 aaaaattatt ctggaaaaag agtattattt attaataaag ttgaaaatga aaaatataaa
     7741 aatttagcta taaaattagt agaaaatatt aaagaatatg aaagtgaaat agagatattt
     7801 tatggaagtg taaaacaaaa attttgtgta agatattgaa gtattaaaat agtttatatg
     7861 atattatata tatagataat atcaatagag atattataaa aatcatagaa ctatcaatat
     7921 attataaata ccaaggatga tatatatgat aaatgcagtg ataatggcat caggctttgc
     7981 cagaagaatg ggagcaaata aacttttatt aaattataaa ggctgttcta taatagaaca
     8041 tgtatttaga gaagttagca agattgattt tcatcaagtt gtagtagtaa gtcaatatga
     8101 agaggttttg ttattgtgta ataaatatgg ttttgaatat atagataata aaaatgcaaa
     8161 tataggtcaa agtgaatcta taaaattagg agttttaaat agctcaaaat gtgatggtta
     8221 tatgttcttt gtaggagacc agccatttat tgataactta tatatagata aaattataaa
     8281 tacttttaaa ttagatagag attttatagt aattccaaga tacaaagata tgtgtggaaa
     8341 tccagtcata ttcccatata gtaagaaaga agaattgttg gcacttaaag aagatgaaaa
     8401 aggtaaaacc attttaaaaa attcatcaaa aataaagtat gtagaagttc ctaaagaaat
     8461 gttatttgat atcgatacaa aaatggatta tgagagaatt gggggtaaat tatgaagcta
     8521 ataaaaacta tagatgctgt tggacaagtt ttatgtcacg acataactca aataatacct
     8581 ggagaattta aaggaagaaa atttaaaaag ggccacatag taaaagaaga agatattcca
     8641 gtgttattat ctcttggtaa agataatttg tatgtatggg aaaagtcaga aggaatggtt
     8701 catgaaaatg aaggcgcatt gtttttaaaa gaactaactg ctggagaaaa tttagatttt
     8761 agtgaaataa aagaagggaa aatagatttt atagcagcat gtgatggtct attaaagata
     8821 gatgttgatg cactttttga tttgaattgt gttggagaaa taatgatggc tactcttcat
     8881 aataatttta tagtaaataa aggtttaaaa gtagctggaa caagagtgat acctttagtt
     8941 atagatgaga aaaaattaga agatgcaaaa aaagtagttg gtaatagaaa aatagtaaat
     9001 gtagtacctt ttaaacctaa gaaagtagga attgttacta caggaaatga agtgttttat
     9061 agcagaattg ttgataaatt tggacccgta atagaagaaa aagtaaaagg ctttggttgt
     9121 gaagttatag gacagactat atgcccagat gataaagaaa ttataaagag tgcaataaaa
     9181 gaatttataa gtcaaggagc agaacttatc tgttgtactg gtgggatgag tgtagaccca
     9241 gatgatgtta caccaacagc tataaaagaa actggagctg atttagtgac atatggttca
     9301 ccaatattac caggagctat gtttttactt gcttattatg gagaagttcc aattatgggt
     9361 ataccaggat gcgcaatgta tcacaaaact acagtatttg atatagtttt atctagggtt
     9421 ttaattgatg aaaagcttga taagtatgat atagctagat atggtcatgg tggcctttgt
     9481 atgaactgtg atgtatgtac ttatcctgca tgtaattttg ctaaaatata aagttattaa
     9541 aaaagagtat ttacaaatta agtactcttt ttaaatatga aacaaaacag ctagaatgat
     9601 gttttaagaa aagcatattt atttagaaat tataataact tattttcaat gtgtaaaaga
     9661 atgaataaag ttgcaatttg atttctttat aagtataata taagagattg ttaattttta
     9721 taaatttaca atttttagta ttattttaat aaatatttca caaatttagt ataaaaaata
     9781 tactatttac gtgataatat ataataaaaa tgttatcaaa gttttatgat aaaataaatt
     9841 atgagcaaaa agaaaggaaa atatattaat gttacaagtt acaggtgttg gacttagatt
     9901 tggtgataag gaactattta aagatgttaa cttaaaattc actaaaggta attgctatgg
     9961 aataatagga gctaatggtg cgggaaaatc tactttttta aaaatattat caggagaaat
    10021 agagcctaat acaggtagta tatctataac tgataaagaa agaatgtcag ttttaaaaca
    10081 ggaccacttt gaatatgaag aagaaactgt tttaaatgtt gttataagag gtcatgagag
    10141 actttggaac ataatgaaag aaaaagatgc attgtatatg aaggaagact ttaatgaaga
    10201 agatgggata aaagctgccg aacttgaagg tgagtttgca gagcttgacg gatgggatgc
    10261 agaaactaat gctgagaaaa tacttatggg tcttggcata acgaaagata tgcattacaa
    10321 acaaatgaag gaactagttg gtggggaaaa ggttaaggta ttgttagcac aatcactttt
    10381 tggtaagcct gaaatactat taatggacga acctacaaac cacttagatt ttaaatcaat
    10441 aaactggtta aataatttta taatggattt agaagaatct atagttataa tagtatcaca
    10501 tgatagacat ttcctaaatc aaatatgtac taatatagtt gacgtagatt ttggtaaaat
    10561 acaaatgtac gttggaaact atgatttctg gtatgagtca agtcaattgg ctttacaact
    10621 ggctaaggac caaaataaaa agactgaaga aaagatagct caattaaagg aatttatagc
    10681 tagatttagt tctaatgctt caaaggctaa acaagcaaca tcaagaaaga aacagttaga
    10741 gaaattagaa gttgaagata tacaaccatc aagaagaaga tatccatatg taggatttac
    10801 acctgcaaga gaaattggga atgaagtttt agaagtacac aatttaacta aaactataga
    10861 tggggttaag gttcttgata atgtgtcatt tagattggat agagatgata aagtagtatt
    10921 tatgggagat gaaatagcta ctactgcact tttcaacata gttatgggag aacttgaacc
    10981 agacagtgga gagtataaat ggggagtaac tacttctcaa gattatcttc caaaaaatca
    11041 taataagttt tttgacggag tagaatattc tttggttgac tggcttagac agttctctga
    11101 agaaaaaagt gaaagtttta taagaggatt tttaggtaga atgttattct caggtgaaga
    11161 agcattaaaa gaagctcaag ttatatctgg aggagaaaag gttagatgta tgcttagtaa
    11221 gctaatgctt tcaaatgcaa atgtacttgt attagatgac cctaccaacc acttagacct
    11281 agaaagtata acttctgtaa ataaaggact tgaaaagttc ccaggagtgc ttttatttac
    11341 ttctcatgac catgaattta tatctactat agcaaataga attatagaga taactccaaa
    11401 tggaataatg gatagaaaaa tggactttga tgagtattta gaaagtaaag agatacaaga
    11461 ccaattagct aaaatgtatg gaaaagataa ataattatta gtataaaacc ttttaatatt
    11521 tgagacatgc tatgcatgag tgtgtaaatt tttaatcaaa tattaggagg ttttttatgt
    11581 tgtttaaaca tgtttattag cagaatagag taatattttg tatattaaga atttaaatat
    11641 aatatagtag atgatataat aaaaatatat aacattttgg ggggaataga ttatggttaa
    11701 gaaaaggtta gtagctgtag taattagttt agctatgatt agtgtaggga atttttcata
    11761 tgctcactca ggagggcata attcatcagg agatcatcat aataaaaata aatcagagtt
    11821 aacacgttat tattgtggag gacattcagc acatcaacac aataaaggtt tttgtcctta
    11881 tgctagtaca aaagaaaatt ctggagtaac aatatcttca gtacataacg aaaaaaatca
    11941 taaaaactat tttgcagaaa aagggtataa taaaggttat gaagatgggt gtaaagggct
    12001 ttataattta agtgtcacag aagccaaatc tgataattta tatgaaaaag agattttaaa
    12061 aagttcatat gaaaaaggat atttaattgg atacaatgaa tataaagcaa aaaaagtaga
    12121 tgatgtaatg aagaagatgt tagatattcc aatgttagca agtatataat aatatagtat
    12181 ttaaaaagat attttctata agggaatatc ttttttattg taaagtataa tttttcgtaa
    12241 aaaagtataa taactgaggc gataaattaa aataaaacag attatttaag tagttattaa
    12301 tttgagatag atacatttaa tattaatgaa tctatctcaa taagctaagc taatttaaag
    12361 ctaagataaa ttaatattaa ttagcattaa ataaactttc agaaattttg tcagaagcct
    12421 ccttatccat ttctttaagt acatgagaat aaacgcttag agtagtactt atatttgagt
    12481 gtcctactct ctcggatata acctttatag gtactttaga atttataagt aaagtaacat
    12541 gtgagtgtct taaatcatgg aatctaatat gaggtaattc attcttttct aaaaatcttt
    12601 taaatttttt actcataaca tcttcagcaa taggttcaca tttcttattg aaaaacaaca
    12661 aattattatt atttctaatg gaagatttta ataaattttt attctgtttt attttatatt
    12721 cttttaataa agacattaat tctttgggag aagatatttt tctaacagag ctttctgtct
    12781 ttggctcttt gagaataaca gaaccattaa ttcgggaagt tatcttattt actgttattg
    12841 tattttcttc aaaatcaata ttatcccaag ttagacctaa aacttctgaa attctaagac
    12901 ctaatccaat agccaaatta ataggtaatt ccatttctgt attttttgct aaatctaata
    12961 attttatcat ttgttctcta tcataaatct catttttaaa ttttttatat cttggagcct
    13021 ctatactatc cataacattt tcttttatta atttgagtct ataagctctt tttattgcaa
    13081 gtttcaatat atttatatga atcttaattg attgaggatt tagtttataa gatagtttat
    13141 ctatgtagtt ttgtatgtga accggatgta agtcttgtat tttatatttt ccaatagatg
    13201 gattaatgta attcttacaa atactaacat aattattata tgtagctaaa gatatatttt
    13261 cactatattt atttagaaaa tctaatagaa attctgacac tgtaatttcg cttggtacta
    13321 aaaaaccatc attatataag ctttctttta cctcatttag tcttttactt gcttcccttt
    13381 ttttatcaaa agtacccata tttttttgtt ttcttttacc tgttttttca tcttcaaatt
    13441 ccaggtaaac aacatagttt ttatttcttt ttctaataaa tgcattcata tataacccac
    13501 caatcaaa
//
LOCUS       DAEPIN010000090        12933 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.090, whole genome shotgun sequence.
ACCESSION   DAEPIN010000090 DAEPIN010000000
VERSION     DAEPIN010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12933
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.090"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            333..1394
                     /locus_tag="KR250_003218"
     CDS             333..1394
                     /locus_tag="KR250_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HBH0069111.1"
                     /translation="MRDFLKNYKSSIILLACTIVGAIVGLVLGPKASILKPFGDLFIN
                     ALLMIVVPLVFLSVSSGVASIKNMERFGKIMKGTAIVFMGTTLVIAFLGITSALLFNP
                     SKGLDVSSVKNLMVTQDAETLESKGTLLDQVVSSITVKNFGDLFSSSNLLQLVIFAVL
                     IGTAIAMMGDRGNTIRKLLSEGTEAILKVVSIFMLYAPIGLGCYTATIIGELGSKIIQ
                     GYVRVFVIYSLTAAMYFLLFYSLYAFLSAGKQGFRQFWRNAIKPIATALATSSSSACI
                     PSNLEASKNIGVPDDISETVIPLGINIHKDGSVLAVIIKVAFLSGILGSGMANPATIG
                     QLFITVFVVGVFMAPVAGG"
     gene            1407..1604
                     /locus_tag="KR250_003219"
     CDS             1407..1604
                     /locus_tag="KR250_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HBH0069112.1"
                     /translation="MLVVSIFGFPVEAIPLLTVITTITDIPNTILNTTGNTVSSMLVS
                     RLVEGKDWLIDKTAITTKKIS"
     gene            complement(1768..2472)
                     /locus_tag="KR250_003220"
     CDS             complement(1768..2472)
                     /locus_tag="KR250_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBH0069113.1"
                     /translation="MQQGSDYLKKFLEKHIDLLKKIPLFEGIKSDELEEMLECLGVVD
                     KTYTKDSIIFSVGSEVTSIGIMLKGSAHIIKEDIEGNRNIVAELLPGDLFGEVFACTR
                     LHKSNVTVTTTSSCEVLFIKFKSVTGICSSACVFHNRLVENMLQLIAEKNILLHNKIE
                     LLSRKTTREKLLMYFSKQIEQTGSHQFTIPFSRNEMADFLCVDRSSMSRELGKMRDEG
                     LLSFNKNKFEIFYFKK"
     gene            2637..3362
                     /locus_tag="KR250_003221"
     CDS             2637..3362
                     /locus_tag="KR250_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBH0069114.1"
                     /translation="MMRKIIKIDEEKCNGCGLCVEACHEEAIGMVNGKAKLLRDDYCD
                     GLGDCLPTCPTNAISFEYREAAEYDEAAVKANMEAKKAQKKTLACGCPGSQSKSINRE
                     VSNSMSISNDIVEIKGSQLNQWPVQIKLVPTNASYLKNASLLIAADCTAYAYGNFHNK
                     FMKNKVTLIGCPKLDEVDYAEKLTEILKENDIKNIVVTRMEVPCCGGIVNAVKTALQN
                     SGKMIPWQIVTISVDGKIVDGEI"
     gene            3422..5014
                     /gene="hcp"
                     /locus_tag="KR250_003222"
                     /gene_synonym="priS"
     CDS             3422..5014
                     /gene="hcp"
                     /locus_tag="KR250_003222"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HBH0069115.1"
                     /translation="MEKMFCFQCEQTAGGKGCTINGVCGKKGGTANLQDELTGVLIGL
                     SRATIGNKNRPTSETDKIMIEGLFTTITNVSFDDEAIKRQIEKTEVEKSKLVPRCSDC
                     STTCNRNDNYDMKDLWNDNEDIRSLKSLILFGLRGMAAYAYHAMVLGYTDKDVNEFFY
                     EGLIAVGGDLSIDELLGLVMKTGEINLKCMELLDRANTETYGTPEPTQVSMKIEKGPF
                     IVVTGHDLYDLHKLLEQTKDKGINIYTHGELLPAHAYPKLKEYKHLKGNFGTAWQNQQ
                     KEFDNIPAPILFTTNCLMPVKDSYRDRVFTTEVVAYPGMVHIDEDKDFSSVIEKALEL
                     GGYREDVHMTGINGGDTLTTGFARGTVSSVQDKVVDAVKSGAIRHFLLVGGCDGAKPG
                     RNYYTEFVKQAPKDTVILTLACGKYRFNDLDLGDICGLPRLMDMGQCNDAYSAIKVAV
                     SLANAFECGVNDLPLSMVLSWYEQKAVCILLTLLHLGIKNIYIGPSLPAFLSKNVLDI
                     LVDKFALTPISTPEEDMKKILG"
     gene            5386..6291
                     /locus_tag="KR250_003223"
     CDS             5386..6291
                     /locus_tag="KR250_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HBH0069116.1"
                     /translation="MKNSNFNILAFDGGGLKGALSISILERIVKEYPNLLNNINMFGG
                     TSTGSLIALGLAYGVSPKEIKELYSIENSKYIFNKSYAEILRPKYENKNLKEVLLSIF
                     PEELELKDLNKLVMIPSFYIGNEENAWKPVFYNNMPNSFTKTSKVVDVAMASSAAPVF
                     FPTYNRHVDGGIIATDPSLACIIHAIDSGFKLKNTRLFSIGTGYVYNSIKADTTEWGA
                     IDWIINKEPDLPIISITLEGNSQMSQLFSQKLLGDNYYRLNPKMEKDVAMDDCDALEY
                     LMSLGENCDIKDAFGWIKKKSFYRN"
     gene            6415..7368
                     /gene="msrA"
                     /locus_tag="KR250_003224"
     CDS             6415..7368
                     /gene="msrA"
                     /locus_tag="KR250_003224"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBH0069117.1"
                     /translation="MIKLATFAGGCFWCMVKPFDKYDGVIKVVSGYTGGHVENPTYQE
                     VCSGTTGHLEAIQITYDDDIISYDELLNIFWKQINPTDDGGQFGDRGTQYKTAIFYHD
                     EEQKNIALESREHLENSKIFDSKIVTDIIPATKFYEAEENHQDYYKKRPLNYNMYYKG
                     SGRYNFVKSHWDGNNINREELKKTLTHIQFEVTQNDMTEVPFENEYYDNNKEGIYVDI
                     VSGEVLFSSKDKFDSGCGWPSFSKPVIDKSIIERSDFSQGMYRVEVRSAKGNSHLGHV
                     FDDGPKELGGLRYCINSASLKFIPRENMEALGYGEYLKLFE"
     gene            7521..8651
                     /locus_tag="KR250_003225"
     CDS             7521..8651
                     /locus_tag="KR250_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH0069118.1"
                     /translation="MNLKDFNGKRLKSARIFRAKTIEQLSKETKINKKDLKAFEENKY
                     VPNIENTLKLSNILNFPKEYFYKNENINIVVEDSHFNPQSKLPRVEEISYREKIIIIH
                     KIYSFMENYIKFPKENLPNRKVMSDININDIESLAYKTREFWDLNSTPIVNMVSLLES
                     KGIIISGMNVDRKGATIFTQKQRISKESKYLISLGNDKKSASIRNYTLACELGYIISS
                     ELRIPSKQFSEDEYACAFLLPKESFLKDLTHPEDLDYYVELKKKWIVPISAMIFRAYN
                     LEKINYKKYNYLMNEMDKKGWLIEEPLDKMKGSSPTYLKRAVELLIENKIMSVNSIVS
                     SLEEFGINLYPEDLELLMGLKKGLLSQEVNKKSKVIKFDGKK"
     gene            complement(8768..9736)
                     /locus_tag="KR250_003226"
     CDS             complement(8768..9736)
                     /locus_tag="KR250_003226"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HBH0069119.1"
                     /translation="MAIKPRKISIVGSGHVGSHCGFSLITQGVCDELFMIDIDESKSK
                     AQALDLADAVSYLPHKVHIEKGTFSDCKDSDIVVISVADSSEGPLRRQNTTRLDLLRP
                     TIGMIKSIVKPIVDSGFDGIFVVISNPVDVVTNYIWEKSGFPKNKVIGTGTALDSTRL
                     RRILSEETGIAQQSIQAYSMGEHGDSQMVPWSHVSIGGKPILDMIKDNPSTYSNLDLP
                     SIVEKNKKTGISIINGKDCTEFGIGTALVEIVKAILHNEKKVLPVSTLLEGQYNERNV
                     FAGVPCVIGKDGIEEIIEINMTEYEQNEFNKSCSVLREYIALSKTL"
     gene            10071..10259
                     /locus_tag="KR250_003227"
     CDS             10071..10259
                     /locus_tag="KR250_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069120.1"
                     /translation="MNYHTKEELVEALRVVSSSIINCGKGQKKFSEETSHHTRFKNII
                     EVMYISKSLIMDEISKRD"
     gene            10519..11718
                     /locus_tag="KR250_003228"
     CDS             10519..11718
                     /locus_tag="KR250_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein AlaX-L"
                     /protein_id="HBH0069121.1"
                     /translation="MEKLYYTNQYIRDFTAEIIEIKEVENKYHVLLDKTAFFPGGGGQ
                     FGDLGKIESDDVINVYEDGKKIYHVLDKKPIKIHKVKCSIDWDRREDGMHQHFGQHVL
                     SGCFFKTFNANTTGFHLGNEYSTVDIEGNLEEDKIREIELFANEIIRQNIQVETLLPT
                     KKELKKIWLRRDLPNTDEEIRVIKIGDLDTNACCGVHPKSTGELRMIKIKKWEKHKSS
                     TRIEFLAGKRAVDEVLKRDIYLTKICRYLSCSEEDAINGIVNLNSKVEEALIKKRRLE
                     EVVSTYQVKEMIEKANKVGNISVIKKIYDDEDLKYVNKIANKIAEYENSIALLAVKFN
                     DKINLVFACSKNLKDINMGVLLKDAISLIDGKGGGSKVLAQGGGKNNGNLEATLDYAF
                     MKIEKTL"
     gene            11770..12273
                     /locus_tag="KR250_003229"
     CDS             11770..12273
                     /locus_tag="KR250_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBH0069122.1"
                     /translation="MEQQKDYIELYENEELIEIREARMDDLDTIAKFNYNLAKETEGK
                     ELDMDVLTKGVKALLLDERKGKYHVYTVFDKVVAQIMYTYEWSDWRNGNFLWIQSVYV
                     DKEYRRKGIFNYLFNYIKNICDKDENIVGMRLYVEKENINAKATYESLNMYECDYNMY
                     EYEVIHS"
     gene            12380..12697
                     /locus_tag="KR250_003230"
     CDS             12380..12697
                     /locus_tag="KR250_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069123.1"
                     /translation="MGVLKGMFYVFLFLDLVSIFCFFFNKGKIASNKIVFNAIGVLTF
                     VLCFMLFSYYPNNNLIGKFIASLFFIFGVAGVMLKEKNFLYARLLLTVVIVFSTLRLF
                     VIQ"
BASE COUNT         5029 a         1361 c         2222 g         4321 t
ORIGIN      
        1 tactaaaatt ttatattaaa gtacaaagta ttaaatttga aaataattaa gtaatattat
       61 atatattttt ttatattgtt tgttttatta aaattaatct agaaaaaaag ttttaaaagc
      121 agaattttat taatatttat atatagatat gaactattaa taaaacgtta aaaatacaaa
      181 aaaactatag aaattgacat atattccagt atttgctaaa gtagctgttg gggaaaatta
      241 caaaaattat taatgcgcat tagataattt actttaattt ttttagcatt tggggtaatt
      301 tataatataa agcattagaa aggaaaatat aaatgaggga ctttttaaaa aattataagt
      361 catcaattat acttttagca tgtactatag taggggcgat agtaggatta gtacttggtc
      421 caaaagcttc aatactaaaa ccatttggag atttattcat aaatgctcta ttaatgatag
      481 tagtaccatt agtttttctt agtgtttctt caggtgttgc tagtataaaa aatatggaac
      541 gatttggaaa aataatgaag ggtacagcta tagtatttat gggaacaaca cttgtaattg
      601 catttttagg tattacatct gcattattat ttaatccgtc taaaggatta gatgtatcaa
      661 gtgtaaaaaa tttaatggtg actcaagatg cagaaacttt agaatcaaaa ggaacgttat
      721 tagaccaagt agtatcaagt attacagtta aaaattttgg agatttattt tccagtagta
      781 atttactaca attagttata tttgcagttt taattggaac agcaatagct atgatgggag
      841 ataggggaaa tacaataaga aagcttttat cagaaggaac ggaagcaata ttaaaagtag
      901 taagtatatt catgctttat gctcctatag ggctaggctg ttatacagct acaataattg
      961 gggaacttgg aagtaaaata attcaaggat atgtaagggt gtttgtaatt tattcactaa
     1021 cagcagccat gtatttctta ttattttaca gtctgtatgc ttttttatct gcaggaaaac
     1081 aagggtttag acaattctgg agaaatgcta taaaaccaat agcaacggca ctagctactt
     1141 cttcaagttc agcatgtata ccatcaaatt tagaagctag taaaaatata ggagttcctg
     1201 atgatatatc agaaacagtt atacctcttg gaataaatat acataaagat gggtcagttt
     1261 tagcagtaat aataaaagtg gcatttttat caggtatttt aggtagtgga atggcaaatc
     1321 cagcaacgat aggtcaatta tttataactg tatttgttgt tggagtattt atggctcctg
     1381 tagcaggtgg ctgacttatt ggtgagatgc tcgtggttag catcttcggt tttccagtgg
     1441 aggcaattcc actgttaaca gttataacta ctataactga tattcctaat actatattaa
     1501 atacaactgg aaatacagta tcatcaatgt tagtttcaag attagtagaa ggaaaagatt
     1561 ggcttataga taaaacagca attactacaa agaaaataag ctaattgtac acttaattaa
     1621 taaagttaat aggtatctta attttaataa attagatacc tattaacttt ttaagtaaaa
     1681 atataaattt aagattcaaa tatgatattt ataatttgaa tcaactaata tagtatatat
     1741 tgaaataaat atgtttaaat atctatttta ttttttaaaa taaaagattt caaatttatt
     1801 tttgttaaaa gataacaagc cttcatctcg catcttgcca agctctcttg acatagaact
     1861 tctgtcaaca cataaaaaat cagccatttc atttcttgaa aagggaattg taaactggtg
     1921 gctacctgtt tgttctatct gttttgaaaa atacataagc aatttctcgc gagttgtttt
     1981 acgagaaagt aattctattt tattgtgaag taaaatgttt ttttcagcta ttaattgtag
     2041 catattttca acaagcctat tatgaaatac acatgcagat gaacaaatac cagttacact
     2101 tttaaacttt atgaataaaa cttcgcaaga agatgttgtg gttactgtaa cattactttt
     2161 atgtagtcta gtacatgcga atacctctcc aaataaatct ccaggcaaga gttcagctac
     2221 aatattccta ttcccttcga tatcttcctt tattatatga gcacttcctt taagcataat
     2281 tccaatagaa gtaacttcac ttccaacaga gaatattata ctatcttttg tgtaagtctt
     2341 atctacaaca cctaaacatt ccaacatttc ttcaagttca tcacttttaa ttccttcaaa
     2401 tagaggaatt tttttaagta ggtctatatg cttttctaaa aattttttca aataatcact
     2461 tccttgttgc atatgcaacc gataatttgt tttcattgta ctatactaag tttataaaag
     2521 caaggggata taaaaatcct actacaagaa atttgaaata aagaaatata ggtattaaaa
     2581 atatatatat aagaaatcaa tactttaaat taaaaaaatt ttatgaggtg atataaatga
     2641 tgagaaaaat aattaaaatt gatgaggaaa agtgtaatgg ttgtggacta tgtgttgaag
     2701 cttgtcatga agaagctata ggaatggtaa atggaaaagc aaaactttta agagatgact
     2761 attgtgatgg gctgggagat tgtctgccaa cttgtccaac aaatgcgatt tcatttgagt
     2821 atagagaagc agcagaatat gatgaagctg cagtaaaagc aaatatggaa gcaaagaaag
     2881 cacaaaagaa aactttagca tgtggatgtc caggttctca gtctaagtca ataaatagag
     2941 aggtttcaaa tagtatgtct ataagcaatg atatagtaga gataaaaggt tctcaactta
     3001 atcaatggcc agtacaaata aaattagtac caactaatgc atcatattta aagaatgcaa
     3061 gtcttttaat agcagcagac tgtacagcgt atgcatatgg aaattttcat aataaattta
     3121 tgaaaaataa ggttacatta atcggatgcc ctaaacttga tgaagttgac tatgctgaaa
     3181 aacttacaga gattttaaaa gaaaatgata ttaaaaatat agtagtaaca cgtatggaag
     3241 ttccttgctg tggtggtata gtaaatgctg ttaagacagc tctacaaaat agtggaaaaa
     3301 tgataccatg gcaaatagtt actatatctg tagatggaaa aattgtagat ggagaaattt
     3361 aattaagtga attatagaaa aatataataa attttaataa ataaaaaggg aggaataaaa
     3421 aatggaaaaa atgttttgtt ttcaatgtga acaaacagca ggaggtaaag gttgtactat
     3481 aaatggtgtc tgtggtaaaa aggggggtac agctaactta caagacgaac ttacaggagt
     3541 gttgattggt ctttcaagag caactattgg aaataagaat agaccaacaa gtgaaacaga
     3601 taaaattatg atagaggggc tattcactac tataacgaat gtaagttttg atgatgaagc
     3661 tataaaaaga caaattgaaa aaactgaggt agaaaaaagt aagcttgtac ctagatgttc
     3721 agattgttca acaacttgta atagaaatga taactatgat atgaaggatt tatggaatga
     3781 taatgaggac atacgttcat taaaatcatt aatcttattt ggattaagag gtatggctgc
     3841 ctatgcatat catgcaatgg tacttggtta tacagacaaa gacgtaaatg aatttttcta
     3901 tgaaggatta atagcagttg gaggagactt gtctattgac gaacttttag gtctagttat
     3961 gaaaactgga gaaataaact taaaatgtat ggaattgctt gatagagcaa atactgaaac
     4021 ttatggaaca ccagaaccga ctcaggtatc tatgaaaatc gagaaagggc catttattgt
     4081 agttacagga catgaccttt atgatttaca caaattatta gaacaaacga aagataaggg
     4141 tataaatata tatactcatg gtgagttatt accagcacat gcatatccaa aactaaaaga
     4201 atataaacat ctaaaaggta attttggtac agcatggcaa aaccagcaaa aagaatttga
     4261 taatattcct gctccaatat tatttacaac aaactgttta atgcctgtta aagatagtta
     4321 tagagataga gtatttacta cagaagtagt tgcttatcca ggtatggttc atattgatga
     4381 agataaagat ttttctagtg taatagaaaa agctcttgaa cttggaggat atagagaaga
     4441 tgttcatatg actggtatta atggaggaga tacattaact actggttttg caagaggtac
     4501 agtttcatct gtacaagata aagtagtaga tgctgtaaag agtggagcta taagacactt
     4561 tttattggtt ggaggttgtg atggtgcaaa acctggaaga aattattaca ctgaatttgt
     4621 aaaacaagct cctaaagaca ctgttatatt gacacttgca tgtggaaaat atagatttaa
     4681 tgatttagac cttggggata tatgtggctt acctcgttta atggatatgg gacaatgtaa
     4741 tgatgcttat agtgcaatta aagtggcagt ttcattggca aatgcttttg aatgtggagt
     4801 aaatgatttg cctcttagca tggtactgtc atggtatgag cagaaagctg tttgtatact
     4861 tcttacacta cttcatttag gtatcaaaaa tatctatata gggccttctt tgccagcatt
     4921 cttatctaag aatgtattag atatattggt tgataaattt gcattgacac ctatatctac
     4981 tcctgaagaa gatatgaaga agattttagg ataaataaac atatctatac ttaaagatat
     5041 ttatagttaa aatattttta agtatgccac aattaagata aggggttaaa cttaaaattt
     5101 gattaaaaaa tcaattttga gatgaccctt tatttatttt ttgtaataat tgaaaaataa
     5161 acctgctttc agtatattct ttttaagaat ctttgtatta aatttataat acttagatat
     5221 ataatatctt atatatttta ttttaagtat taaaggtttg aatgtaactt aaatattaaa
     5281 agaggaacat tgtaatatgt aacttgcata aaatatatat actatagtga aattattttg
     5341 attaaaattt aaaatataag gtgttttatg tgggggtata tgtatatgaa aaattctaat
     5401 tttaatatat tggcatttga tggtggtgga ttaaaagggg cattgagtat atccatactt
     5461 gagaggatag taaaagagta tccaaatcta ttaaacaata taaatatgtt tggaggtaca
     5521 tctacaggga gtctaattgc actagggctt gcgtatggtg taagccctaa agagataaaa
     5581 gaactttact caattgaaaa ttcaaagtat atttttaata aaagttatgc agaaatactc
     5641 agaccaaagt atgaaaacaa aaacttaaaa gaagttttat taagtatatt tccagaggaa
     5701 ctagaattaa aagatttaaa taaattagta atgataccaa gtttttatat aggaaatgag
     5761 gaaaatgcat ggaaaccagt gttttataac aatatgccaa attcttttac aaagactagc
     5821 aaagttgtag atgtagcaat ggcaagtagt gctgctccgg tattttttcc aacatataat
     5881 cgtcatgttg atggaggaat aatagcaact gacccgagtt tagcgtgtat aattcatgcc
     5941 attgacagtg gatttaaact taagaataca agactttttt caatagggac tggatatgta
     6001 tataacagta taaaggcaga tactactgaa tggggagcta ttgactggat aattaataaa
     6061 gaacccgatt taccaattat atctattacc ttagaaggaa attctcaaat gagtcagtta
     6121 ttttctcaaa aacttcttgg agataattac tatagattaa atcctaagat ggaaaaagat
     6181 gtagctatgg atgattgtga tgcattagaa tatctaatgt ctttaggaga aaattgtgat
     6241 ataaaagatg cttttggatg gataaagaaa aagagctttt atagaaatta gattcaattt
     6301 gcaattgtat ttttacacat gatttcattg agtaggtaaa aataataaaa tgcagattat
     6361 taagttactt cttagggaat aaataaagta taatttattt aaggagtgat gaaaatgata
     6421 aaacttgcta cttttgcagg tggatgtttt tggtgtatgg taaaaccttt tgataagtat
     6481 gatggagtta ttaaagtagt ttctggatat acaggtggtc atgttgaaaa ccctacatat
     6541 caagaggtat gctcaggaac tactggtcat ttagaagcaa ttcaaataac atatgatgat
     6601 gatataataa gttatgatga acttctaaat atcttctgga aacagattaa tcctacagat
     6661 gatggtggac aatttggtga taggggaact caatataaga ctgctatatt ttatcatgat
     6721 gaagaacaaa aaaatatagc tttagagtca agagaacatc tggaaaatag taaaatattt
     6781 gatagtaaaa tagtgactga tataattcca gcaactaaat tctacgaagc agaagaaaat
     6841 catcaggatt attataagaa aagaccatta aactacaata tgtattataa agggtctgga
     6901 agatataatt ttgtaaaaag ccattgggat ggaaacaata taaataggga agagttaaaa
     6961 aagactttaa ctcatataca atttgaagtc actcaaaatg atatgacaga agtacctttt
     7021 gaaaatgaat attatgacaa taacaaagag ggaatttatg ttgatattgt aagtggagaa
     7081 gtgttatttt cttcaaaaga taaatttgat tctggatgtg ggtggccaag tttttcaaag
     7141 ccagttatag ataaatctat cattgagaga agtgatttct cacaaggtat gtacagagtt
     7201 gaagtaagaa gtgctaaagg aaattctcat ttaggtcatg tatttgatga tggtccaaaa
     7261 gaacttggag gtttaagata ttgtataaat tctgcttctc ttaaatttat accaagagaa
     7321 aatatggaag ctttaggata tggagaatat ttaaaacttt ttgagtaatt aggaatattt
     7381 attataaact tatctaaaag gtgaaaatat aaaaaaatat tttcaccttt tgtttttttg
     7441 tcaataatat gataaattat taattgtaat cttatttata ccaatagatt aagaatttaa
     7501 ttttgaaaga gagaaaacaa atgaatttaa aagactttaa tggaaagaga ttaaaatcag
     7561 ctagaatttt tagagcaaag acgatagaac agttgtctaa agaaactaaa ataaataaaa
     7621 aagatttaaa agcttttgag gaaaataagt atgtaccaaa tatagaaaat acattaaaat
     7681 tatctaatat attaaatttt ccaaaagagt atttttataa aaatgaaaat ataaatatcg
     7741 ttgtagagga tagccacttt aaccctcaat ctaaactacc tagagtagaa gagatttctt
     7801 atagagaaaa aataattata atacataaaa tatattcatt tatggagaat tatattaagt
     7861 ttccaaaaga aaacttgcca aatagaaaag taatgtcaga tattaatata aatgatatag
     7921 aatctcttgc atataagact agagagtttt gggatttaaa tagtactcca atagtaaata
     7981 tggtaagttt gttagagtct aaagggatta taatatcagg tatgaatgta gatagaaaag
     8041 gggcaactat ctttactcaa aaacagagaa ttagtaaaga atctaaatat ctaatatctc
     8101 tgggtaatga caaaaagtca gcatcaatta gaaattatac tttggcatgt gagcttggat
     8161 atataatatc aagtgaatta agaataccaa gtaagcaatt ttcagaagat gagtatgcct
     8221 gtgcattttt attgccaaaa gagagttttt taaaagattt gactcatcca gaagatttag
     8281 attattatgt agaactaaag aaaaaatgga ttgtacctat atctgctatg atttttagag
     8341 catataatct agaaaagata aattacaaaa aatacaatta tcttatgaat gaaatggata
     8401 aaaaaggctg gttgatagaa gaaccactag acaaaatgaa aggttcaagc ccaacgtatt
     8461 taaaaagagc agttgaatta ttgatagaaa ataaaataat gagtgtaaat agtatagtat
     8521 caagtttaga ggaatttgga ataaatcttt atccagaaga cttagaatta ttgatgggat
     8581 tgaaaaaagg attgttatct caagaggtaa ataaaaaaag taaagttata aaatttgatg
     8641 gtaaaaaata ggtttatatt ttagtattaa aattaaaagc aggtacaaat gatttaaaca
     8701 ttaacttagt taaaagtctt ttcaataagt catttacctg cttttagtgt aagaaagaat
     8761 attgaatcta aagtgttttt gaaagtgcaa tatattccct taaaacagaa caagatttat
     8821 taaattcgtt ttgctcatat tcagtcatat tgatttcaat aatttcttca ataccatctt
     8881 ttcctataac acatggaaca cctgcaaata catttctttc gttatattga ccttctagta
     8941 atgtagaaac tggaagaact tttttctcat tgtgtagtat agcttttaca atttctacta
     9001 aagcagtacc tataccaaat tctgtacaat ctttaccatt tattatactt attcctgttt
     9061 ttttattttt ttcaactatt gatggtaagt ctaaattgct ataagtactt gggttgtctt
     9121 ttatcatgtc taagattggt ttaccaccaa ttgaaacatg agaccaagga accatttgag
     9181 aatctccatg ttcacccata gaataagctt gaattgattg ttgagcgatt ccagtttctt
     9241 cagataaaat ccttctaagt cttgttgaat ctaaagctgt tccagttcca atgactttgt
     9301 ttttaggaaa gccagatttt tcccaaatat aatttgtaac tacatcaaca ggattagata
     9361 tgactacaaa tataccatca aagccagaat caactatggg ttttactata gattttatca
     9421 ttccaatagt tggtcttagt aaatctaatc tagttgtgtt ttgtctacgt aaaggccctt
     9481 ctgaagagtc tgcaacactt atcactacta tgtcagaatc tttacagtca gaaaaagttc
     9541 ctttttctat atgtacttta tgtggcagat atgaaactgc atcagctaaa tctaaagctt
     9601 gagcttttga tttagattca tctatatcaa tcataaagag ttcatcacaa actccttgag
     9661 ttattaaact aaatccacaa tgagaaccaa catgccctga acctacaata gatatttttc
     9721 ttggctttat tgccatttta aatcactccc cctaaaataa cgatacattg aaaattatag
     9781 atgttattaa aagctgtttt tggatatttt aatattctat tatttcagaa aatttattta
     9841 atatgatttt atactaaaat ttttaaaaaa gcaatgttaa tatactctct attagtgaat
     9901 attaaagtat tatatgtata ttaataatta ttaaaatatg taatgtcact gatagttaga
     9961 atatttaagt gctaaaagat gtgtataaat aataatgatg tcagtttata atgatattaa
    10021 attgagatat actgtttctc taaattaaaa aataaaggag taaatgtgtt atgaattatc
    10081 atacaaaaga agaattggtg gaagcactga gagtagtatc ttcaagtatt attaattgtg
    10141 gaaaaggaca gaagaaattt tctgaggaga catctcatca tacacgtttt aagaatatta
    10201 tagaagtaat gtatatttca aaatcattaa taatggatga aattagtaaa agagattgaa
    10261 gcttattttg caatttaaca tgttttaata tacattttat tatataagaa aataagtaaa
    10321 catacatgtt ataaaatatt ggaataatat acaattataa atttgttaac actaatatct
    10381 atatgttaaa atatagtaga agatgttagt aaataatagg atgagttcta ttttaataca
    10441 attaaattaa gcttagtttg aaatttgtat ctaaaaagta aagtaatcta gaattaagaa
    10501 aatggagata gaattataat ggaaaagtta tattatacaa atcagtatat aagagatttt
    10561 acagcagaaa taattgaaat aaaagaagta gaaaataagt atcatgtttt attagataag
    10621 acagcttttt ttcctggtgg aggaggtcag tttggagacc tgggaaaaat agagagtgat
    10681 gatgtaataa atgtgtatga agatggaaaa aaaatatacc atgtattaga taaaaaacct
    10741 ataaagatac ataaggtaaa gtgtagtatt gattgggata gaagagaaga tggaatgcat
    10801 caacattttg gacaacatgt attgtctgga tgttttttta aaacttttaa tgcaaatacg
    10861 acaggttttc atttgggaaa tgagtatagt acagtagata tagaaggtaa tttagaagag
    10921 gataaaatta gagagataga gttgtttgcc aatgaaatta ttcgtcaaaa tatacaagta
    10981 gagacattac ttccaacgaa aaaagaacta aaaaagattt ggttaagacg tgatttgcca
    11041 aatactgatg aagagataag agtaataaaa ataggtgatt tagatacgaa tgcatgttgt
    11101 ggagtgcatc caaaatcaac tggtgaattg agaatgataa aaataaagaa atgggaaaaa
    11161 cataagtcaa gtacaagaat agagtttcta gcaggtaaaa gggcagtgga tgaagtattg
    11221 aagagggata tatatttgac taaaatatgt agatatttaa gctgtagtga ggaagatgct
    11281 ataaatggta ttgtaaatct aaatagtaaa gtggaagagg ctttaattaa aaaaagaagg
    11341 cttgaagaag tagtatctac atatcaggtc aaagaaatga ttgaaaaagc aaataaggtt
    11401 ggcaatattt cggtcattaa aaaaatttat gatgatgaag atttgaaata tgtaaataag
    11461 atagcaaata aaattgcaga atatgaaaat agtattgcat tattagctgt taaatttaat
    11521 gataaaataa atttagtttt tgcatgttct aaaaatttaa aagatattaa tatgggagtt
    11581 ttattaaagg atgcaataag tcttatagat ggcaagggtg gtggaagtaa agtattagct
    11641 caaggaggag gtaagaataa tggaaattta gaggctacac ttgattatgc atttatgaag
    11701 atagaaaaaa ctctataaat atattatata aaaaacctta gtagataaaa tagatatgga
    11761 ggaataagta tggagcaaca aaaggactac atagagttat atgaaaatga agaactaata
    11821 gagataagag aagcacgtat ggatgatttg gatacaatag caaaatttaa ttataatttg
    11881 gctaaagaaa ctgaaggaaa agaacttgat atggatgttt taactaaagg tgtaaaagca
    11941 ttgttattag atgagagaaa aggaaaatat catgtatata cagtatttga taaagttgtt
    12001 gcacagatta tgtatacata tgagtggagt gattggagaa atggaaattt cctttggatt
    12061 cagagtgtat atgtagataa ggaatataga agaaaaggta tctttaatta tttatttaat
    12121 tatataaaaa atatatgtga taaagatgaa aatatagttg gaatgagatt atatgtagaa
    12181 aaagaaaaca taaatgcaaa agcaacatat gagtctttaa atatgtatga gtgtgattat
    12241 aatatgtatg aatacgaagt aatacattcg taatataaat ttgtaatact gttatactga
    12301 tgttataaag tattgattat attgacgatt atatgaaata aactggatat tataattaga
    12361 atttttaggg ggtataaaaa tgggcgtttt aaaaggaatg ttttatgtat ttttattttt
    12421 agacttagtg tcaatattct gtttcttttt caataaagga aaaatagcat ctaataaaat
    12481 tgtgtttaat gcaataggag tacttacatt tgtactttgt tttatgcttt ttagttatta
    12541 tcctaataat aatcttattg gaaaatttat tgcttctcta ttttttatat ttggagttgc
    12601 aggagttatg ttgaaagaaa aaaatttttt atatgctaga cttcttttaa ctgtagttat
    12661 agttttttct acattaagat tgttcgttat tcaataaaat gtatttgtat gaaaatttat
    12721 taaagtctac acatttaagt gtaggctttt ttgatttttt gtttgaaaat taaggaaatt
    12781 ttaaggaaat tttaaaatcg tcttaaatat ttattgatat acttaaaata ttaatagcga
    12841 aaggagactt atcagtgata gaaagtaaaa agtcagaaaa attgttactg tcaattatat
    12901 acataatatt tttggtatat attttaatga tta
//
LOCUS       DAEPIN010000091        12792 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.091, whole genome shotgun sequence.
ACCESSION   DAEPIN010000091 DAEPIN010000000
VERSION     DAEPIN010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12792
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.091"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            complement(265..969)
                     /locus_tag="KR250_003231"
     CDS             complement(265..969)
                     /locus_tag="KR250_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B3/4 domain-containing protein"
                     /protein_id="HBH0069124.1"
                     /translation="MKKFVIEDAFWNLFPSAKIGVVICNDIDNSIKDEDYKKIILKGE
                     EEALKYLEDSEFSNNQVIKVWREAFKKFKTKKGARSSIEALLKRVHNGNNIGTINPLV
                     DIYNFISLKYALPCGGEDIDKFIGDIRLTRAIGNEEFIPLGTDENSFPYEGEIIYKDD
                     GGAICRCWNWREAVRTMLTEDTNNAFLCIELVDESRFKEFENALNELAKIVQEKLGGN
                     CKISILDIDNKEVSVD"
     gene            complement(1323..2339)
                     /locus_tag="KR250_003232"
     CDS             complement(1323..2339)
                     /locus_tag="KR250_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HBH0069125.1"
                     /translation="MGYYNDNDNNKIRRKKISNNKDSNKKSKHSYTSANKKNKSPNTS
                     SSKRMSEKVDSQNLIKRDRDSFTRDNVRPLNTDYNQRERRRVEYKQREKKLKSKKRKK
                     QKRIRIVRALLSIMLVALIIFSVAGAIFTVSAIKGSPEVTKKLIRSKYISSEVVSIKQ
                     MPNDLKHAIVSIEDERFYKHKGVDVISLVRSVLNNVFTDTTQGASTIDMQVSKNLLTS
                     NDKTMKRKIRDMYNAKQMNKIMTKDEILEAYLNNMYLGKNAYGAAKGAEVYFGKKVSE
                     LNLAECAMLAGMTNNPARYIDHGEAKKRQVIVLYKMHQLGYITDKEYRIAKADDTPFK
                     SEID"
     gene            complement(2467..2973)
                     /locus_tag="KR250_003233"
     CDS             complement(2467..2973)
                     /locus_tag="KR250_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HBH0069126.1"
                     /translation="MKEYVVVDLETTGLDPYKGCEIIEIGITEIKNEQIVKNYSRLIK
                     PKGIISSFITELTHISNEMVENEEPLELVLPRFRKYIGDRTIIAHNAKFDLKFLNYYL
                     RMLDLEPINNYICTVELLKKCKSYKGKNKKLETACAYYNIENINAHRADSDTLATAKL
                     FLKIKDEY"
     gene            complement(3132..3800)
                     /locus_tag="KR250_003234"
     CDS             complement(3132..3800)
                     /locus_tag="KR250_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069127.1"
                     /translation="MENITSYFTTILCVFICLSSVFIFTQLARVFINKKKINQKIKSR
                     NGFRYDRDFIEARREEIHIKDNNNNKNKSNNKKLKEEKVFKYDNGDLYKGEFVDGKKN
                     GFGIYIFSGKEKYEGLWKDDKMHGIGKYTYRDGSIYTGEFKYGLKNGLGKLTYPNNDI
                     YKGYFLDNKKNGKGVLYKNDGNKQAGIWEDDEQCKSLDFKDLNNNKINNVYQNRLNNN
                     KLKV"
     gene            complement(4213..4719)
                     /locus_tag="KR250_003235"
     CDS             complement(4213..4719)
                     /locus_tag="KR250_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HBH0069128.1"
                     /translation="MGFREVKIEELQFNPFTKIGKEWLLITAGDSEKFNTMTASWGGV
                     GVYWGKNVVTTYIRPQRYTKEFVDSNDTFTIAFFDETYREALNICGTISGRDINKIEK
                     AGLTPYFVDDTVAFEEANMIIVCKKLYHGNMPPENFDAKENDKKWYPEKDYHTMYISE
                     IIKVLVKE"
     gene            complement(5090..6118)
                     /locus_tag="KR250_003236"
     CDS             complement(5090..6118)
                     /locus_tag="KR250_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HBH0069129.1"
                     /translation="MKRVYIDFEMNMPNSKSKRAVFNSDIIAIGAVMYDEKTKNIDKF
                     KSLIRPVSNEELYPHIQELTHISSEELKSAPSYEEVMRKFKKWLGIFSDIKGIYTFGN
                     LDLTCFNNTDMRSAKKNNHPRFVNNIRDLFVDIKEKYINCGMRCMNYISLKNLLEFVN
                     LEFSGDAHDPLNDAYNLFILDEAITNNVDIQNLLIIRDIIRPPFNDINSNLDNCFNKF
                     KESLYKKEGNYNIVDFSVEIIKTVRMYLLTIIDVNIQNLEIMKDISKKMDTIDKLKDI
                     EEGYFYLLEDVYFDMKDVLEDLMLYRMNEDEYKYEIKNIIKMFDEDLDNEKIYINKCN
                     NLNVVNKI"
     gene            complement(6166..7026)
                     /locus_tag="KR250_003237"
     CDS             complement(6166..7026)
                     /locus_tag="KR250_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HBH0069130.1"
                     /translation="MSELSGKGCEIIVPFEERLPVRDIEKSIIKKYRKNLWSKFMKAI
                     RDYKLVEEGDKIAVAISGGKDSILMAKMFQELKKHGQVNFDVEFIAMDPGYHANIRQL
                     LIDNCEYLNIPIHLFDSRIFEIADEIAKDYPCYMCARMRRGALYSKAEELGCNKLALG
                     HHYDDVIETTMLNLLCAGNFKTMLPKLNSTNFEGIKIIRPLYYIREEHIIRFIQNSGI
                     WPLNCACMVAAKKTGNKRYEIKDLIKSLESNFKNVEKSIFKAAENVNLDSVLGWQKDG
                     EKHSFLENFE"
     gene            7295..7504
                     /locus_tag="KR250_003238"
     CDS             7295..7504
                     /locus_tag="KR250_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat associated protein CotJA"
                     /protein_id="HBH0069131.1"
                     /translation="MENKTRDIKKNCGPSLARPYINNQIFTEMYCLSDALKFGTIFPE
                     LNLLESEMYNKELYAKPKKYRGGRR"
     gene            7504..7770
                     /locus_tag="KR250_003239"
     CDS             7504..7770
                     /locus_tag="KR250_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein CotJB"
                     /protein_id="HBH0069132.1"
                     /translation="MKDSSRAELMRKVQETSFACVDMNLYLDNHPDDKNAINTYNSLC
                     NQFAQARYAYENKYGPLTNFGYAPSRCPWQWADQPWPWDREFNY"
     gene            7799..8371
                     /locus_tag="KR250_003240"
     CDS             7799..8371
                     /locus_tag="KR250_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000265278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HBH0069133.1"
                     /translation="MWIYQKTIQHPVNIKTCDPRMAKFLITQFGGPNGELAASLRYLS
                     QRYTMPTGNMRALLTDIGTEELAHVELICTMVYQLTSDASPEELKAAGLGSNYAQNGY
                     GIYPTDSNGVPFDVRPIAVMSNPVTDLHEDMAAEQKALATYYQLINLTDDVDVIDVLK
                     FLGQREIIHYQRFGEALMDAYELEESQKMF"
     gene            complement(8606..9796)
                     /locus_tag="KR250_003241"
     CDS             complement(8606..9796)
                     /locus_tag="KR250_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HBH0069134.1"
                     /translation="MNFNQKRIAASIMATAIIMPTMGNLAYANESEVESVSIESRTIT
                     GNAVNFRKGPGTNHESMGKLYKGDKVEYVGKEGSWVKVKYNGNTGYVHGNYVAINSLG
                     SSNESSDTSVKSTKVVTAKGLNFRTGPSTSSSKISTLGYGTEVGYISESNGWSKISSN
                     GRVGYVSSKYLGTSVNDSTNENAENSSNDLVKGTKVVTAKSLNVRTGPGTSHSKIATL
                     SYGTEVGSISESGGWTKISYGNQTGYVSSQYLAEKGSVDTSIPSYSTNSPSQGADSVI
                     SFAKTLLGKPYVWGAEGPNSFDCSGFTQYVMKKSVGVSIPRVSRDQSKYGTYVNRGDL
                     RSGDLVFFDTQGSNNGSVSHVGIYIGNGDMIHASSGSSKKVTISNINSSYYSSRYVNA
                     RRVL"
     gene            complement(10056..10487)
                     /gene="dut"
                     /locus_tag="KR250_003242"
     CDS             complement(10056..10487)
                     /gene="dut"
                     /locus_tag="KR250_003242"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HBH0069135.1"
                     /translation="MYNLKVKKLNDDAIIPNFAHKGDAGMDLYSIEEVVIPPGETKLI
                     KTGICIELPTMTEAQVRPRSGLALKHSVTVLNTPGTIDEGYRGELKIILINHGKNDFK
                     VEKHMKIAQMIVKPIYDINIEEVKELSDSERGKGGFGSTGF"
     gene            10600..>12792
                     /locus_tag="KR250_003243"
     CDS             10600..>12792
                     /locus_tag="KR250_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
                     /protein_id="HBH0069136.1"
                     /translation="MKLVLAEKPSVAKTIASFLGAKTRQDGYFEGNDYIITYAVGHLV
                     SLYDMKDYDKDKYSGSWKMNNFPFVPEDKFKFKIDSSKTKQFNTVKKLLNRKDVEYVI
                     NATDNDREGELIAFLIFLLAKNKKPVKRILVNEWTPEDITRGIKNLKDEDEMRNLQAA
                     GYTRLITDWLIGINFTSVATLKYGNGKLLNIGRVILPTVKLVYDRDMEILNFVPKTYY
                     EIEGHFKAEAGEYKGKYVKGKESKFDTLEDANKIIASITSETGKILDKKVTMSKEYAP
                     KLFSLTSLQGYITSKYSNFTSDKVLNVCQSLYEGNGKGGYITYPRTDSIFLEESLASK
                     ASQTLDKLKVGLEYENKIKFSKTKRVFDSSKVDSHSAIIPTYIIPKSLSKDEQLVYDA
                     IKDRFVANFMPPAEYENTEIKTEVDNCTFLTKGKVLKSKGYLEVYNKEEKNDLLPLVN
                     KDDVVDVLEIKPLTKQTTPPKPYTEDTLLKAMKNCGKNVPEEDTTVLSGYSIGTSATR
                     ADVLKKISQVGYVKKKGKSYSITDLGKNLVEIFPVKDLFDVDYTGKLEKSLSDIQKGK
                     YTRKEYLTNIISFIYQNVNIIKQDASKNINTEAYTFDSKTKKFTKTKEKKQKKDVTNT
                     EKEVAISKDSNNKKEVKNGDENQSLGKCPICQGDVLEFDKGFACKNHKECKFVIWKND
                     KYLSLYKKKVNKTMVKNILKKGETKVKSLTAKNGNKFDATLKYDKNPDT"
BASE COUNT         4345 a         2060 c         1409 g         4978 t
ORIGIN      
        1 tagtataata cattatatta tatcgttttt aaataaaaat attataatta acaatatatt
       61 tttattgtta attatgcaaa taaaaaagag tataataaaa gcctttaaaa tacatttttt
      121 tcttatttta aaggcttttt atattctaca tttttatttt ctatattata tcctttgata
      181 atcgttatat ctaaaataga ccttcttacc ctaaatataa gtatctttat aactagaata
      241 gtccattaca acagcacctc catattaatc cactgatacc tctttattat caatatctaa
      301 aattgaaatt ttacaattcc cacccaattt ttcttgaact attttagcta attcatttaa
      361 agcattttca aattctttaa atctactttc atctactaat tcaatgcata aaaaggcatt
      421 gttagtatct tcagtaagca ttgttctcac tgcttcacgc caattccaac atctacaaat
      481 tgctccacca tcatctttat aaattatttc tccttcatat ggaaatgaat tttcatctgt
      541 ccctagtggg ataaactcct cattaccaat tgctctagtt agcctaatat ctcctataaa
      601 tttatctata tcctcccctc cacaaggtaa tgcatatttt aatgagataa aattgtagat
      661 atccactaaa gggttaatag ttcctatatt atttccatta tgaactcttt ttaataatgc
      721 ttcaatagaa gaccttgctc cttttttcgt tttaaatttt ttaaatgctt ctctccatac
      781 tttaatcact tggttgttgc taaattctga gtcctccaag tattttaatg cttcttcttc
      841 tcccttcaaa attattttct tatagtcttc atcttttata gagttatcta tatcattaca
      901 aataacaact ccaatttttg cactaggaaa tagattccaa aatgcatcct caataacaaa
      961 ttttttcatt agaaaacctc ctcaaattta aaatatgtaa aaataaaaaa gcctatagaa
     1021 atattcttaa atatctccca ggcttttatc cttccgtgta cacaacaatt gcgcgttttc
     1081 tcttggacct gaccattata aaatgcggaa ccctagaaaa ctaaattata agttttttaa
     1141 tttacaaaat acaattaaat tttagcataa tatagcattt aagacaatat tctattatct
     1201 agtttgtaca ttaaaacaat ttgccattag attataatta tggtggcaaa tgggtggcaa
     1261 atcacctttt ttattacatt tttgtttgtt taaactattg aaaatactat attcttacaa
     1321 ttttaatcaa tttctgattt aaatggagtg tcatcagctt ttgctattct atactcctta
     1381 tcagttatat aaccaagctg atgcatctta tacagtacaa tcacttgtct cttcttagcc
     1441 tctccatggt caatatacct tgcaggatta tttgtcatac cagctaacat agcacactca
     1501 gctaaattta attcactaac ttttttacca aagtagactt cagctccctt tgcagctcca
     1561 tatgcatttt tacctagata catattattt aaatatgctt caagaatttc atccttagtc
     1621 ataatcttat tcatctgctt agcattatac atatccctta ttttccgctt catagtttta
     1681 tcattactag taagcaggtt ttttgaaact tgcatatcta tagtacttgc accttgtgtt
     1741 gtatctgtaa acacattgtt taatactgac ctaactagag aaataacatc tactccttta
     1801 tgcttataaa atctctcatc ttctatggaa actattgcat gttttaaatc atttggcatt
     1861 tgttttatac ttacaacctc actacttata tatttagacc taattaactt tttagtaact
     1921 tctggtgacc cttttatagc tgaaactgta aatattgcac ctgccactga aaatataatt
     1981 agtgcaacta acataataga taataatgcc ctaactattc taatcctttt ttgttttttt
     2041 ctttttttac tttttaattt tttctctctt tgtttatact cgactcttct tctttctctt
     2101 tgattgtaat ctgtatttaa aggtcttaca ttatctcttg taaaactatc tctatctctt
     2161 tttataagat tctgtgaatc tactttttca ctcattcttt tactggaact tgtattagga
     2221 cttttatttt ttttatttgc cgaagtataa gaatgtttag attttttatt actatctttg
     2281 ttgttactaa tttttttacg tcttatttta ttattatcat tatcattgta ataacccatt
     2341 ttagtctccc ttcttcatat aaggtacatt atacattgtt ctacaaaata aatcaaagaa
     2401 caaaattgta agcttaaggg tcaattttat aaaggatatt ttcactaatt ttctctatat
     2461 ttataattaa tattcatctt ttattttcaa aaatagtttt gcagttgcta atgtatcact
     2521 gtcagctcta tgagcattta tattctctat gttgtaataa gcacaagctg tttcaagttt
     2581 cttgtttttt cctttataac ttttacattt ttttaataac tctactgtac atatataatt
     2641 gtttattggt tctaaatcta acattctaag atagtaattt aaaaatttta aatcaaattt
     2701 cgcattgtgt gcaattatag ttctatcacc tatgtatttt ctaaatctag gtaaaactaa
     2761 ttctagtggt tcttcatttt ctaccatttc attacttata tgtgtcaatt ctgttataaa
     2821 tgaagaaatt atccccttag gttttatcaa ccttgaatag ttttttacaa tttgttcatt
     2881 ttttatttct gttattccta tttctattat ttcacaacct ttatatggat ctaatcctgt
     2941 agtttctaaa tccacaacta catactcttt catatctctt ctccttatta ttaaatggat
     3001 atatttatat tctatattta tgtaatagac taatcaatac ttaattttat atgatatagg
     3061 aagattttac aaattacaac atagatatat cctatattcc atcataaata aagatttttt
     3121 tattagaata attaaacttt taatttattg ttatttaatc tattttgata aacattattt
     3181 attttattat tatttaaatc tttaaaatct aaacttttac attgttcatc atcttcccat
     3241 attcctgctt gtttatttcc atcattttta tataatacac ccttaccatt tttcttatta
     3301 tctaaaaaat atcccttata aatgtcatta ttgggatatg ttaattttcc caaaccattt
     3361 tttaatccgt atttaaattc acctgtatat attgaaccat ctctataagt atattttcct
     3421 attccatgca tcttgtcatc tttccataat ccttcatact tttccttacc tgaaaaaata
     3481 tatattccaa atccattttt ctttccatca acaaactcac ctttatataa atcaccatta
     3541 tcgtatttaa atactttttc ttcctttaat ttcttattgt tactcttatt tttattatta
     3601 ttgttatcct ttatatgtat ttcttctctt ctggcttcaa taaaatctct atcataccta
     3661 aatccgtttc ttgatttaat tttttgatta atcttcttct tattaataaa tactctagct
     3721 agttgagtaa atataaatac agatgataag catataaata cacatagtat tgttgtaaaa
     3781 taactagtta tattttccat aaaaaatcac cctttaaaaa atttatttaa aatacctaat
     3841 ataatatata tttttattaa atgttattta tacttttata gtactatatt gaataaaatt
     3901 aactttgaaa ttatttacat taaaataaat caagtacaaa tgccataaat taagtgtttc
     3961 tagttagcat aaaaatactt ataaagtata ctcaaatatt tcaaataaat acttttattc
     4021 aaataaatct ctacttatat aagtgcttat ataaataaaa agatgaacta tagtaaatat
     4081 agttcatctt ttttataata gtatttattt aaaattaata tttatttagt tagtagcatc
     4141 tacttagata tcagataaga aagcacagtc atttacttat aataagttct tttcatatat
     4201 tttttattat aattattctt ttacaagaac ttttataatt tctgaaatgt acatagtatg
     4261 atagtctttc tctggatacc attttttatc attttcttta gcatcaaagt tttctggtgg
     4321 catattacca tgatatagct ttttacatac tataatcata ttagcttctt caaaagctac
     4381 cgtatcatcc acaaaatatg gtgtaagtcc tgctttttct attttattta tatctctacc
     4441 agaaatagtg ccacaaatat ttaaagcttc tctatacgtt tcatcaaaga aggcaattgt
     4501 aaatgtatca ttactatcta caaattcttt tgtatatctc tgtggtctta tgtaagttgt
     4561 aacaacattt ttaccccaat agactccaac accaccccag ctagcagtca ttgtgttaaa
     4621 cttttctgaa tcaccagctg ttataagtaa ccattctttg ccaatcttag taaatggatt
     4681 aaactgtaat tcctcaattt ttacttctct aaaacccatg acttcatcct acccttcatt
     4741 ttttaattat ataaattaat tgttaaaaat ttgaatatat gtactcttat aatactgact
     4801 tttgattgtc ctacataatt catgtaaatt ataatacaac aaatcaatta ctttcgcaat
     4861 aagtagtttt catattttat aagtgtaaaa tatagataaa atatattgat tataaagtat
     4921 ataatatatt tttaaaccta aagcaatata caataaaaaa atgtagaaag taagtatctc
     4981 tctacatttt ttattgtata ttatttttat attaatttaa ttttatcttg attcagtttt
     5041 ataaaaaatt gttataattg tcaagttcta ctctattatt tataatttct tatatcttat
     5101 taacaacatt taagttatta catttattta tatatatttt ttcattatct aagtcttcat
     5161 caaacatttt tattatattt tttatctcat acttgtactc atcttcattc attctataaa
     5221 gcattaaatc ttctagtaca tctttcatat cgaagtacac atcttctaat aaataaaaat
     5281 agccttcctc tatatctttc aatttatcta tagtatccat ttttttactt atatctttca
     5341 taatttctaa attttgtata tttacatcta tgattgttaa taagtacatt ctcacagttt
     5401 ttattatttc aacagaaaaa tctacaatat tataatttcc ttctttttta tataatgact
     5461 ccttaaattt gttaaaacaa ttatctaaat tagaatttat atcgttaaat ggtggtctta
     5521 ttatgtcacg tattattaaa agattttgta tatctacatt atttgtgatt gcctcatcca
     5581 aaataaataa attatatgca tcatttaatg ggtcatgggc atctccacta aactctaagt
     5641 taacaaattc aagtaaattt ttcaatgata tatagttcat acatctcata ccacaattta
     5701 tatatttttc ttttatatct acaaataaat ctctaatgtt atttacaaac cttggatggt
     5761 tatttttttt agcacttctc atatctgtgt tattgaaaca tgtcaaatcc aaattaccaa
     5821 aagtatatat tcctttaata tcagaaaata tgccaagcca ttttttaaac tttctcatga
     5881 cctcttcata acttggtgca ctcttcaatt cttcagaaga tatatgtgtt aattcttgta
     5941 tatgaggata caactcctca ttacttactg gtcttattaa agatttaaat ttatcaatat
     6001 tttttgtttt ttcatcatac ataactgctc cgattgcaat tatatcagaa ttaaatactg
     6061 ctctttttga tttagaattt ggcatattca tttcaaaatc tatatatact cttttcaata
     6121 taaacaccct ttccaattgt ttatagtgat tctttttaac tatttttatt caaaattttc
     6181 taagaaagaa tgtttctccc catctttttg ccaaccaagt actgaatcaa gatttacatt
     6241 ttctgccgcc ttaaatatag atttttctac atttttaaaa tttgattcta agctctttat
     6301 taagtctttt atctcatatc ttttattacc tgttttttta gcagcaacca tacatgcaca
     6361 atttaatggc catataccac tattttgaat aaatcttata atatgttctt ctcttatata
     6421 ataaagtggt cttataattt ttataccttc aaaattagta gaattaagtt taggaagcat
     6481 tgttttaaaa tttccagcac acaataaatt aagcattgtt gtctctatta catcgtcata
     6541 atgatgacct aatgcaagtt tgttacaccc taattcttct gcctttgaat ataatgctcc
     6601 tctcctcatt cttgcacaca tatagcatgg ataatctttt gctatttcat ctgctatctc
     6661 aaatatcctt gaatcaaata aatgtattgg tatatttaga tactcacaat tatctataag
     6721 taattgtctt atatttgcat gatatccagg gtccatagca ataaattcaa catcaaaatt
     6781 aacttgtcca tgttttttta actcttgaaa cattttagcc ataagaatac tgtcttttcc
     6841 accagaaatc gctacagcta tcttatcacc ttcttcaacc aatttataat ctcttattgc
     6901 cttcataaac ttagaccata aatttttcct atattttttt attatacttt tttctatatc
     6961 tcttactgga agtctttcct caaaaggaac tataatttca cagccttttc ctgatagttc
     7021 actcatctaa tcacctcgta tatttatgtt aataatattt tacaaaaaag tctaccttca
     7081 tccacaaaat actataattt ttactttaat tttactaaat aatttatagt ttacaataac
     7141 acataaactt ataataacat aaataacctc tttcttttat tatctttttt atttttctca
     7201 tttaatattt tattattagt aacgattttt aaatgattga atattataca ctagatagag
     7261 ttacctatct atttacaaca aggaggtttt catcttggaa aataagacta gagatatcaa
     7321 aaaaaattgt ggtccatctt tggctagacc atacattaat aatcaaattt tcacagaaat
     7381 gtactgcctt tctgatgcat taaaatttgg aactatattt ccagaactaa acttactaga
     7441 gtctgaaatg tacaacaaag aattatatgc taagccaaaa aaatatagag gaggtagaag
     7501 ataatgaaag actcatctag agctgaactt atgagaaaag ttcaggaaac atcttttgca
     7561 tgtgttgata tgaacttata tttagacaat caccctgatg ataaaaatgc tatcaacact
     7621 tataattcat tatgtaatca atttgcgcaa gctagatatg cttacgaaaa taaatacggt
     7681 cctttaacta actttgggta tgctccaagt agatgccctt ggcaatgggc tgaccaacct
     7741 tggccttggg acagagaatt taattattag acatcaagta ttggaggatt gaaaattcat
     7801 gtggatatat cagaaaacta tacaacaccc agttaatata aaaacttgtg accctagaat
     7861 ggctaaattt cttataactc aatttggtgg gccaaatgga gaacttgctg catctttaag
     7921 atatttaagc caaagatata caatgcctac tggtaatatg cgtgcacttt taacagatat
     7981 tggtacagaa gaactagctc acgttgagct tatatgtact atggtttatc agttaacttc
     8041 tgatgcaagc ccagaagagt taaaagctgc aggtcttggt tcaaactatg ctcaaaatgg
     8101 atatggaatt tatccaacag attcaaatgg tgttccattt gatgtaagac ctatagcagt
     8161 tatgtcaaat cccgtaaccg atttacatga ggatatggca gctgaacaaa aagcacttgc
     8221 aacttattat caacttataa acctaacaga tgacgttgat gttatagatg tattaaaatt
     8281 cttgggtcaa agagaaataa ttcattatca aagatttggt gaagccttaa tggatgctta
     8341 cgagttagaa gaatctcaaa aaatgttcta aaatttttat ttgtataact attattattt
     8401 gataatttat aatttactgt tgtgttaaaa tttataaaaa atataaatat aatttatttt
     8461 ctataaacag agaaaataaa taaaaagcag gtatcaatca aatagtaaca tgtattatac
     8521 tatacattac tatcaatatt gtacctgctt tcatattata agcaataaag aaaaatctat
     8581 ttattaggtt ttataataaa aacaattata aaactcttct tgcatttaca tatctacttg
     8641 aatagtagct actatttata ttagatattg ttactttttt tgatgaacct gaagatgcat
     8701 gtatcatatc tccatttcct atgtatattc caacatgact tacagaacca ttatttgaac
     8761 cttgtgtatc aaagaatact aaatctccac ttctaaggtc tcctctattt acatatgttc
     8821 catacttact ttggtctcta gatactcttg gtatactaac tcctacagat tttttcataa
     8881 cgtactgcgt gaatccagaa cagtcaaaac tatttggacc ttctgctccc catacatatg
     8941 gttttccaag tagtgtcttt gcaaaactta ttacactatc agctccttga cttggagaat
     9001 tagtcgaata agatggaatt gaagtatcaa cagaaccttt ttcagcaagg tattgacttg
     9061 acacatatcc agtttgattg ccataactta tttttgtcca tcctccactt tcagaaatac
     9121 ttccaacctc agtaccataa cttaatgtag ctattttaga atgacttgta cctggtcctg
     9181 ttctaacgtt caatgacttt gctgttacaa cctttgtacc ctttacaagg tcatttgaac
     9241 tgttttcagc attttcattt gtagagtcat ttacactagt tcctaaatat ttacttgaaa
     9301 cataacctac tctaccatta gaacttattt ttgaccatcc atttgactca gatatgtatc
     9361 cgacttcagt cccatatcct agtgttgata ttttagaact acttgtactt ggtcctgttc
     9421 taaaatttaa tccttttgct gttacaactt ttgtcgactt aactgaagta tcactacttt
     9481 cattgctact tccaaggcta tttattgcta cataatttcc atgcacataa cctgtgtttc
     9541 cattatattt aacttttacc caagaacctt cttttcctac gtactcaact ttatcacctt
     9601 tgtaaagttt ccccattgat tcatggtttg tacctggtcc ttttctaaaa tttaccgcat
     9661 tccctgttat tgttctgctt tctatactca cactctctac ttcagactca ttagcatatg
     9721 ctaaattccc catagtaggc attattatcg cagttgccat tatactggct gctattctct
     9781 tttgattaaa attcataact ttccactcat ctcctctttt ctactatctt tatttcactt
     9841 caaaatataa aagaaataat tgacactatc aaaatatcaa ttatttcttt tatattaata
     9901 ttattacaaa tttgtaacta tttgtctata gtaatatttg cttaccataa attaccatgt
     9961 ataaatttta atctttaata gcctgtaatt tagtaatata gccatctttt acctttttta
    10021 aaaatggcta tattttttaa cttttttgta actttttaaa atccagttga gccaaaccca
    10081 ccttttcctc tttcggagtc acttaattct tttacttctt ctatgttaat atcatagata
    10141 ggttttacta tcatttgagc aattttcata tgcttttcaa ccttaaaatc atttttacca
    10201 tgatttatta atataatttt aagctctcct ctgtatcctt cgtcaatagt tcctggtgta
    10261 tttaatacag taacagaatg ctttaaggca agcccacttc taggtcttac ctgcgcttct
    10321 gtcatagtag gaagctctat acatatccca gttttgataa gtttagtctc tccaggaggt
    10381 attacaacct cttctataga atacaaatcc ataccagcat cacctttgtg agcaaaatta
    10441 ggtattatag catcatcatt taattttttt acttttaaat tgtacatctt atatctcctt
    10501 tcaactttct ataaatatta taacttaaat ttcgacctct tgaagttttt tatttttttg
    10561 tggtaatata attattagtg ttttaaagga ggatttttta tgaaacttgt acttgctgag
    10621 aaaccatctg tggcaaaaac tatagcatca tttttaggtg caaagaccag acaagatggt
    10681 tactttgaag gaaatgatta tataattact tatgcagttg gacacttagt atcattatat
    10741 gatatgaagg attatgataa agataaatac tctggctctt ggaaaatgaa caatttccca
    10801 tttgttcctg aggataagtt taaatttaag attgatagtt ctaaaactaa acaattcaac
    10861 actgtaaaaa agcttttgaa tagaaaagat gtagaatatg taataaatgc cactgataat
    10921 gaccgagaag gagaacttat agcattttta atatttttac tggcaaaaaa taaaaaacca
    10981 gtaaaaagaa tcttggtaaa tgaatggaca ccagaagata ttacaagagg tataaaaaac
    11041 ctaaaagatg aagatgaaat gagaaatctt caagctgctg gatatactag gcttattact
    11101 gactggttga ttggaataaa tttcacttct gttgctacat tgaaatacgg aaatgggaaa
    11161 ctattaaaca taggtagggt aattcttcca acagttaaat tagtctatga tagagatatg
    11221 gaaatactaa actttgttcc taaaacttat tatgaaatag aagggcattt taaagctgaa
    11281 gctggtgaat ataaaggtaa atatgttaag ggtaaagaaa gtaaatttga tacacttgaa
    11341 gatgcaaata aaattatagc ttcaattaca tctgaaactg gtaaaatact agataaaaaa
    11401 gttactatgt caaaggaata tgcaccaaaa ctttttagtt tgacatcact tcaaggatat
    11461 ataacttcta agtattccaa ttttacatct gataaggttt taaatgtctg tcaatctctc
    11521 tatgagggta atggtaaggg tggatacata acttacccta gaactgattc cattttctta
    11581 gaagaaagtt tggcttcaaa agcttctcaa actttagata aattaaaggt tggtcttgaa
    11641 tatgaaaata aaattaaatt ttctaaaact aaaagagttt ttgattcttc taaagtagac
    11701 tcccatagtg ctataattcc aacttatata attccaaaaa gtttatctaa ggatgaacaa
    11761 ttagtttatg atgccataaa agatagattt gtagcaaact ttatgcctcc tgctgaatat
    11821 gagaatacag aaattaagac tgaagtagat aattgtactt tcttaacaaa aggtaaggtg
    11881 cttaaatcaa aaggatacct tgaggtatat aataaggaag aaaaaaacga tttacttcca
    11941 cttgtaaata aagatgatgt tgtagatgtg ttagaaataa aaccattaac taagcaaact
    12001 actccaccta aaccgtatac tgaggacaca cttttaaagg ctatgaaaaa ttgtggtaaa
    12061 aatgtgcctg aagaagatac aacagtgcta tctggatatt cgattggaac atcagccaca
    12121 cgtgctgatg tgttaaagaa gataagtcaa gtgggctatg taaagaaaaa aggtaaatct
    12181 tattctataa cagaccttgg gaaaaattta gttgaaattt ttcctgttaa agacttattt
    12241 gatgtagatt atactggaaa acttgaaaaa agtttaagcg atattcaaaa aggaaagtat
    12301 acaagaaaag aatatcttac taatatcatt agttttatat atcaaaatgt caatattata
    12361 aaacaagatg cttcaaaaaa tataaacaca gaagcatata ctttcgattc aaaaactaaa
    12421 aaatttacta aaacaaaaga aaaaaaacag aaaaaagatg taactaatac tgaaaaagaa
    12481 gttgctatat caaaggattc taataacaaa aaagaagtta aaaatggtga tgaaaatcaa
    12541 tctcttggca aatgccccat ttgtcaaggt gatgtactag aatttgacaa aggctttgct
    12601 tgtaaaaatc ataaagaatg taaatttgtt atttggaaaa atgataagta tttaagtctt
    12661 tataaaaaga aagttaacaa aacaatggtc aaaaatattt taaaaaaagg tgaaactaaa
    12721 gtaaaatctt taactgctaa aaacggtaat aaatttgatg ctacattaaa atatgataaa
    12781 aatccagata ct
//
LOCUS       DAEPIN010000092        12433 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.092, whole genome shotgun sequence.
ACCESSION   DAEPIN010000092 DAEPIN010000000
VERSION     DAEPIN010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12433
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.092"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            272..973
                     /locus_tag="KR250_003244"
     CDS             272..973
                     /locus_tag="KR250_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanG-Cd-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HBH0069137.1"
                     /translation="MNENILIVDDEKEIADLIEVYLKNEGFTVYKFYTGKEALECIES
                     KNLDMAILDIMLPDIDGFHICQKIREKYYYPIIMLTAKIEDIDKIMGLTIGADDYITK
                     PFNPLEVVARVKTQLRRYMRYNNSYEQQSIIVNEYDIKGLIINKETHKCSLFGKEVAL
                     TPIEFSILWYLCEHQGKVVPSEELFEAVWGEKYLDNNNTVMAHIGRLREKIHEPSKNP
                     KFIKTVWGVGYTIEK"
     gene            1023..2105
                     /locus_tag="KR250_003245"
     CDS             1023..2105
                     /locus_tag="KR250_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBH0069138.1"
                     /translation="MRTALMAFIAIIFIWILYSFILYGKFSIWIVSFFENAFRMEYKA
                     ALNLYQQIFRNSMELFMILAMVIVFFIIFRVYLNWFTKYFSEINQGIDSLIKEDVGEV
                     ALSPELLAIEKKINSIKHILEQRKFETQMAEQRKNELIVYLAHDLKTPLTSVIGYLTL
                     LRDESQISEELRKKYLSISLDKAERLEDLINEFFEITRFNLSNISLEYSTVSLTRMLE
                     QLTYEFKPTLMSKNLKYTLNVTPNMMIRCDVNKMQRVFDNLLRNAVYYSFEDTTIEIT
                     AVQEDDYIKLKFINHSNTIPEEKLERIFEQFYRLDTSRNSNSGRAGLGLAIAKEIVEL
                     HNGTITAHSENNIITFKVIIPIVGNL"
     gene            2366..3466
                     /gene="vanG"
                     /locus_tag="KR250_003246"
     CDS             2366..3466
                     /gene="vanG"
                     /locus_tag="KR250_003246"
                     /EC_number="6.3.2.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-serine ligase VanG"
                     /protein_id="HBH0069139.1"
                     /translation="MNNKKIAVIFGGNSTEYDVSLQSAFAVLENLNTEKYDIIPIGIT
                     REGNWYHYIGDYNNIQNNTWFERDTDIIPVIVSQNRVKNGIIEFLIPDAVSQNHIPNS
                     AIESCENDIKYTKLDFVFPVLHGKNGEDGTVQGLFELAGIQVIGCGTLSSALCMDKDK
                     SHKLVHSEGIKVPKSILLKYSDKFNVAELSKDLTYPLFIKPVRAGSSFGISKIYKKEE
                     LQVAVDLAFEHDNEVIIEENIDGFEVGCAVLGNNDLLVGRVDEIELSKGFFDYTEKYT
                     LKSSKIHMPARIDLDCEKRIQETAKIIYRTLGCSGFARVDMFLTPSGEIIFNEVNTIP
                     GFTSHSRYPNMMKGIGLSFEEILDKIIEVYIK"
     gene            3463..4269
                     /locus_tag="KR250_003247"
     CDS             3463..4269
                     /locus_tag="KR250_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBH0069140.1"
                     /translation="MMNNILLDKNKEDKMTNTILLTKDKVYDGNLILVNAFLPVKTSE
                     DIDLIPVDTRFPSILMKREATNILQNILKSICGINEIVPVSGYRTAEEQQDIYSSSLR
                     DNGKDFTKKFVALPNHSEHQTGLAIDLGLKKEVIDFICPEFPYNGICNEFRKAALHYG
                     FIERYQCKKEEITGISQEPWHFRYVGYPHSEIINKMNLALEEYIEYIKRFSYTNCLKI
                     EDKEKIIEIFYVPIHTKEEVRISIPEQCVYQISGNNKDGFIVTFWRNRKD"
     gene            4287..6425
                     /gene="vanT"
                     /locus_tag="KR250_003248"
     CDS             4287..6425
                     /gene="vanT"
                     /locus_tag="KR250_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine racemase VanT catalytic subunit"
                     /protein_id="HBH0069141.1"
                     /translation="MIRNKSYAGLDYFRIIAALLIIAIHTSPLLSYSKMADFVLTSII
                     ARIAVPFFFMLSGFFLISTYNYNCNKLKSFLKNVLFIYGIAIVIYIPLNIYTGYFSMN
                     NLVPNIIKDIVFDGTLYHLWYLPASIIGASIAYFLVKKLGLKYSLVITIVIYVIGMFG
                     DSYYGFLEKIPLLKNLYGYIFEVSDYTRNGIFFAPVFFILGGIISNKSNYMSTKNSLI
                     GFLISFLLMLCEGMFLHKLGVQRHDSMYIMLLPCMYFLFTALTFLKGSRARLLRTSSL
                     IIYIIHPMMIVVIRMFSKIFGIQTVLVDNSLIHYIVVSIASFIASIIIILILKPLKKD
                     LKGEHKVNTNRAWIEIDLNNLEHNVKMIQKAMPEDCELMAVVKANAYGHSAFEVATCA
                     NQIGVKAFAVATIDEGIDLRRYGILGEILILGYTNPIRAKELHKYNLTQSVIDLNHAI
                     SLNKQKYNIKSHIKIDTGMHRLGFDVNDVRSILKAFKLKYLDICGIFTHLCVSDSLKD
                     EDVDFTNKQIECFYKLIDFLLKKGIKIPKVHIQSSYGFFNYPNLKCNYVRIGIALYGI
                     LSFPNDTTKLQLDLRPVLSLKSQVILIRKIQKDDSFGYGRVFIASRDSVIAILSIGYA
                     DGIPRNLSCGKSYVIINGCRAAIVGRICMDQLAVDITDIPNVEVGNTAIIIGRDNLSE
                     LSVSEVANNSCSISNELLSRVGRRLNVIKK"
     gene            6739..7359
                     /locus_tag="KR250_003249"
     CDS             6739..7359
                     /locus_tag="KR250_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBH0069142.1"
                     /translation="MQYIQELLDLTQNYWVLATIIGLLSAFIESFIPALPLVAIVTAN
                     AAIQGLFIGCLLSWIGSGLGTTSLFLLISRFNDSKLFNKLRNSKTEKAISWMDKQGFK
                     LLFIAYACPFMPGCLVTIASAFGKKDLKDFVPAMLAGKFVMFIVISYVASDIEGFITS
                     PLKIASFILLVFLSWKIGNRVNKNLENHNYDFHHKKHHNDKDDKMI"
     gene            complement(7525..8061)
                     /locus_tag="KR250_003250"
     CDS             complement(7525..8061)
                     /locus_tag="KR250_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBH0069143.1"
                     /translation="MIKETHERGGYIFALLILPFINNLYLVKYDIAYKIVLIIIYIYF
                     AYLGSLFPDIDMRGSYISKKFMLIYKLFGSRFRHRGFTHSLIALLLIYSFFKSLTIFT
                     NNNIVFSCLSSGFIIGYLSHLCLDLITKEGIELFYPITINISLLPIKTNSKTEKFISK
                     LLNFIVIFLIGYQFYILL"
     gene            complement(8174..8848)
                     /locus_tag="KR250_003251"
     CDS             complement(8174..8848)
                     /locus_tag="KR250_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HBH0069144.1"
                     /translation="MSLFIISQCITSFAFTPENNKFKVKPLPYAYDALEPYIDKETMK
                     LHHDKHYQAYVDKLNAALEKYPELYNYSLCELLQNLDSLPKDIATTVRNNAGGAYNHK
                     FFFDIMTPEKTIPSESLKEAIDRDFGSFEKFKQEFQKSALDVFGSGWAWLVATKDGKL
                     SIMTTPNQDSPVSKNLTPIIGLDVWEHAYYLKYQNRRNEYIDNWFNVVNWNGALENYK
                     NLKSQD"
     gene            9051..10925
                     /gene="asnB"
                     /locus_tag="KR250_003252"
     CDS             9051..10925
                     /gene="asnB"
                     /locus_tag="KR250_003252"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HBH0069145.1"
                     /translation="MDGNISIYYKGDISKKEDIKFKIKESVNNTRLSSRIEYKDEEKT
                     FDFERFNVIDAIYGNKPFEKDGKVIVFNGNIYNYHEIKEELIKKGHNFTTNDDIEVLL
                     ASYIEFGKNCVHKIKGMFNFIIYDRENESIFGARDLFGIKPLYYINKENAIIFSSEYK
                     FLLEYMKNLNINERSLQSYFSFQYVLPEDTMIQGIRLIPAGHYFRVENGILSLKRYNK
                     LEFRSSTKFFYTKNHLGNRDVNEDDIRNIVVDSIVTHMEEEKEIGTFLSGGIDSSIIT
                     TVASQINPNIKSFSAGFSVKGYSELEVAKKTADKLGIENIQINITQDEYIKSLPNVIY
                     SLDDPIADPSEVGLYFLIKEAGKHVKVALSGEGADELFGGYNIYKEYSTMKSVVNSPT
                     YIKSILGRVSELMPNIKGRNYLYRATTPLEKRYIGNAKVFENSEVKRFFFKYKEKNIY
                     EYLLSNLYRDAQKNNYDYISKMQHIDVNTWLQGDILQKISKLSTAEQVELRVPFLYKD
                     VFDVAKNLRMEQKINKNNTKVLLREAFREIVPEHVVQRKKLGFPTPIRVWLKDSLGDI
                     VKETIYNSNVDEFIDKKYAIKLLDTHLKGHRDNSRKIWTIFTFCLWHQLFIEHKNVEY
                     "
     gene            complement(11017..>12433)
                     /locus_tag="KR250_003253"
     CDS             complement(11017..>12433)
                     /locus_tag="KR250_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HBH0069146.1"
                     /translation="LKISIKKSTEPLIEFISDTTYKNKRSSYIKEVYFTRYIGVDDSI
                     DKYIEKIKNIDTYFFNNPTIPYIRLNNLKINFDKEQTDKMLKIFNIYNSSKFNSWDLY
                     NIDFPCKIENDTLNWTKKIAFKNTLDLFSTSMPNCTSSIIKNFAVKLLCWIDYFLPKL
                     FYNKVKTTISPKIVYFGNIKRQELFFLYFLSQLGCDILYINPNKDILDLYPECSKFST
                     LFELSRKTPNILEIPFERHITNINYSENTNKTSPTTNKPNSKNTLNNTNLTSKKIVQD
                     EEVELSYEDLAKLSASVVMIVVCNNENKPFKSGSGVVINNEGYILTNLHVVNDGYSFL
                     VRFENDDKVYTSYQIIKYHSDYDLAVIKVDRKCKPIPVKVSKKPVRGQKIVAIGSPLG
                     LFNTVSDGIISAFRDFETVQMIQFTAPISSGSSGGALLDMFGNLLGLISAGYDDGQNL
                     NLAVESNLVKIFANNFIEIVN"
BASE COUNT         4657 a         1425 c         2002 g         4349 t
ORIGIN      
        1 ctctggtata ataaattgaa ttgtgtttga ctgatagata tttttattga aagttaatac
       61 ctgttatgcg ataataagga cgtgttaggg gagtttttat aataacaatt aatctcagtt
      121 tttgtgttcc aatagtagca tcaacacatt ctgatgtagt agttcttttc ttataaaaat
      181 tagttacaaa tatgcttgta attatttgat atgtaaattt accaatatta taaatgatga
      241 cctcgataat aaggagagtg aaaaataatc tatgaacgaa aatattttga ttgtggatga
      301 tgaaaaagaa atagcagatt taattgaagt atatttaaaa aatgaaggtt ttacagtata
      361 caagttttat acaggtaagg aagctctaga atgtattgaa tctaaaaatt tggatatggc
      421 tattttagat attatgcttc ctgatattga tggatttcat atttgccaga aaattcgaga
      481 aaaatactat tatcctatca ttatgttaac agctaaaatt gaagatatag acaagataat
      541 gggtttaacc ataggtgctg atgattatat aacaaagcca tttaaccctt tggaagttgt
      601 tgcaagagta aagacacaat taagaagata tatgcgatat aataattctt atgaacaaca
      661 gtcgataatt gttaatgagt atgatattaa aggattgatt atcaataaag aaacacataa
      721 atgtagttta tttgggaaag aagtagcatt aacaccgatt gaattttcta ttctttggta
      781 tttatgtgaa catcaaggaa aagtggttcc ttctgaagaa ttgtttgaag ctgtatgggg
      841 agaaaaatat cttgataata ataacacagt tatggctcat atagggcgat tacgtgaaaa
      901 aattcatgaa ccttctaaaa acccgaaatt tataaaaact gtatggggag taggttatac
      961 aattgaaaag taaaaatatt aaagtcaatc ctgactttac tcaagtgaga aataaaattt
     1021 ttatgagaac agctttaatg gcatttatag ccattatttt catatggatt ttatattctt
     1081 ttatattgta tggtaagttt tctatctgga tagtatcatt ctttgaaaat gcttttcgta
     1141 tggaatataa agctgcattg aatttatatc agcagatttt ccgtaatagt atggagctct
     1201 tcatgatact agctatggtg attgtgtttt ttataatatt ccgtgtttat ttaaattggt
     1261 ttacaaaata ttttagtgaa ataaatcaag gtattgactc tcttatcaaa gaagatgttg
     1321 gagaagtggc tttatctcca gagttattgg caatagaaaa gaaaataaac tcaattaaac
     1381 atatacttga acaacgtaaa tttgagacac aaatggcaga acaacgtaag aatgaactta
     1441 ttgtttatct tgcacatgat ttaaaaacac cacttacatc agttataggg tatctgacac
     1501 tacttcgaga tgaaagtcag atttctgaag aactgcgaaa aaaatattta tctatttcgt
     1561 tggataaggc agagcgtctt gaagatttaa ttaatgaatt ctttgaaatt acaaggttta
     1621 atttatcaaa tatatcactt gaatacagta cagtaagctt aactcgtatg cttgaacaac
     1681 taacatatga gtttaaacct actttgatga gtaagaattt aaaatataca ctgaatgtta
     1741 caccaaatat gatgataagg tgtgatgtta ataaaatgca acgtgtattt gataacttat
     1801 tacgaaatgc tgtttattat agttttgaag atactactat tgaaataaca gctgtacagg
     1861 aggatgatta tataaagctt aaatttataa atcatagcaa tacgattcca gaggaaaaac
     1921 ttgagcgtat ttttgaacaa ttttacaggt tggatacatc tcgtaattca aacagtggta
     1981 gagctgggtt gggacttgct atagctaaag aaatagtgga gcttcataac gggacaataa
     2041 cagctcatag cgagaacaat ataattacct ttaaggttat tattccgatt gtaggaaatt
     2101 tgtaagaatt taagtataaa aaaataggaa attctttagg ataaataaat aaagagcact
     2161 cttgttattg atataattac tgtatcaaaa caagagtgct tttattttga tacagtaatt
     2221 atatcaatag aaaaatgtga aaatatatta tgtggaacag atgcaattgt aagtgaaggt
     2281 tgattggatt tgaaatagaa aaagtatttt aacatgtttc tgtagattgt ataaaaataa
     2341 ataaatcaac aaaggagaat caaagatgaa taataaaaaa attgcagtta tatttggtgg
     2401 aaactcaaca gaatatgatg tatcattaca atcagctttt gcagtacttg agaatttgaa
     2461 tacggaaaaa tatgatatta taccaattgg aattacaagg gagggtaatt ggtatcatta
     2521 tattggagat tataataata tccagaataa cacttggttt gaaagggata ctgatataat
     2581 tccagttatt gtttcgcaga accgtgtcaa aaatggtatt attgaatttt taattcctga
     2641 tgctgtttct cagaatcata ttccaaatag tgctattgaa tcatgtgaaa atgatattaa
     2701 atacactaaa cttgattttg tgtttcctgt actgcatgga aaaaatggag aagatggaac
     2761 agtgcagggg ttatttgaat tggcaggtat acaggttatt ggatgtggta ctctttcttc
     2821 agcattatgt atggacaaag acaaatcaca taaacttgtt cattcagaag gtattaaagt
     2881 accaaaatca atattactaa aatactcaga taaatttaat gtggctgaac tgtcaaaaga
     2941 cttaacttat ccattattta taaaacctgt gcgtgcaggt tcatcatttg gtattagtaa
     3001 aatttataaa aaagaagagt tacaagtagc agtagacttg gcttttgaac atgataatga
     3061 agtaataatt gaggaaaata ttgatggttt tgaggtaggg tgtgcagttt taggaaacaa
     3121 tgacttactt gttggaagag tagatgaaat agaattgtca aaaggatttt ttgattatac
     3181 agaaaaatat acattgaaaa gttcaaaaat tcatatgcca gctagaattg atttggattg
     3241 tgagaaaaga atacaagaga cagcaaaaat tatttataga actcttggat gttctggatt
     3301 tgctagagta gatatgtttt taactccatc tggtgaaatt atttttaatg aagtcaatac
     3361 aattcctggc tttacttctc atagtcgtta tccaaatatg atgaaaggta ttggactgtc
     3421 ttttgaagaa atattggaca agataattga ggtgtacata aaatgatgaa taatatttta
     3481 ttagataaaa ataaagaaga taaaatgaca aatactatct tattgactaa agataaagtt
     3541 tatgatggga atctaattct agtaaatgct tttttaccag ttaaaacatc tgaagatata
     3601 gatttaattc ctgtagatac aaggtttcca agtattctta tgaaacgtga agctacaaac
     3661 attttacaaa atattttaaa aagtatatgt ggaataaatg aaatagtacc tgtaagtgga
     3721 tatcgtactg cagaggaaca acaagatata tattcaagtt ctctgcgtga caatggaaaa
     3781 gactttacta aaaagtttgt agcacttcca aatcacagtg aacatcagac agggcttgct
     3841 attgatttag ggttaaagaa agaagttata gattttatct gccctgaatt tccttataat
     3901 ggtatttgta atgagtttcg taaggcagca ctacattatg gttttataga gcgttatcag
     3961 tgtaaaaaag aagaaataac aggaatatct caagaaccat ggcattttcg ttatgtggga
     4021 tatccgcatt ctgagattat caataaaatg aatctcgctc ttgaagaata tatagaatat
     4081 attaagagat tttcgtatac aaattgttta aaaatagaag ataaagaaaa aattatagaa
     4141 atcttttatg tgcctattca tactaaagaa gaagtgagaa tctctatacc tgaacaatgt
     4201 gtatatcaaa tatcgggtaa caataaggat ggttttattg tgacattttg gagaaataga
     4261 aaggactaag aaatggagga acaagaatga ttaggaataa atcttatgca gggcttgatt
     4321 attttaggat tatagctgca ttacttataa ttgcaattca tacatctcca ttattatcat
     4381 atagtaaaat ggctgacttt gtgttaacta gtataattgc tcgtattgct gtgccattct
     4441 tttttatgct atcaggcttt tttctaattt caacatataa ttataattgt aataaactga
     4501 aatcttttct aaaaaatgta ttatttattt acgggatagc tattgtaatt tatattccct
     4561 taaatattta cactggatat ttttccatga ataatttagt gcccaatatt attaaggaca
     4621 ttgtatttga tgggacttta tatcatttat ggtatcttcc tgcatcaatt attggtgcaa
     4681 gtattgcata ctttcttgta aaaaaactag gtttaaagta ctcactagta atcactattg
     4741 ttatttatgt aattggtatg tttggtgata gttattatgg ttttttagaa aaaataccac
     4801 tattaaagaa tttatatggg tatatttttg aagtatcaga ttatacacga aatggtatat
     4861 tttttgcacc tgtatttttc atacttggtg gaataatatc taataaatct aattatatgt
     4921 caactaaaaa tagcttgatt ggatttctta tatcattctt acttatgtta tgtgagggaa
     4981 tgtttttaca taaactaggg gtacagcgtc atgatagcat gtatattatg ttattgccat
     5041 gtatgtattt cttatttaca gccttaacat ttttgaaagg ttctcgagct aggttattgc
     5101 gaacatcatc attaattatt tatattatac atcctatgat gattgttgtt attcgtatgt
     5161 tttctaaaat attcggaata caaactgtct tggttgacaa tagccttatt cattatatag
     5221 tggtaagtat agcatctttt atagcttcta taattattat attgattctt aaacctctaa
     5281 aaaaagactt gaaaggagaa cataaggtaa ataccaatag agcttggatt gaaattgatt
     5341 taaataattt ggaacataat gtaaagatga tacaaaaagc tatgccagaa gattgtgaat
     5401 tgatggcagt tgtaaaagct aatgcctatg gtcatagtgc ttttgaagta gcgacttgtg
     5461 caaaccaaat aggtgtaaaa gcatttgcag tagcaacaat agatgagggg attgatttac
     5521 gtcgatatgg aatactagga gaaatattga ttcttggata tactaaccca ataagggcaa
     5581 aggaacttca taaatacaat cttacacaga gtgttattga ccttaatcat gctatctctt
     5641 taaacaaaca gaaatacaac ataaaatcac acattaaaat tgatacaggt atgcacagac
     5701 tgggttttga tgttaatgat gtgaggtcaa ttttaaaggc atttaaatta aaatacttag
     5761 atatatgtgg catatttaca cacttgtgtg tttcagatag tctgaaagat gaagatgtag
     5821 actttacaaa taaacaaatt gaatgttttt ataagttaat tgattttctg ttaaaaaaag
     5881 gtataaaaat tcctaaagta catattcaaa gtagttatgg tttttttaat tatcccaatc
     5941 ttaagtgtaa ttatgtaaga attggaattg cattatacgg gatattaagt tttccaaatg
     6001 atacgactaa attgcaactt gatttacgac ctgttttatc cttaaaatca caagtaattc
     6061 ttattagaaa aatacaaaaa gatgatagtt ttggatatgg aagagtcttt attgcaagca
     6121 gagatagcgt aattgctata ttatctattg gctatgccga tggtatacct agaaatttat
     6181 cctgtggaaa aagttacgta ataataaatg gttgtagagc agcaatagtg ggtcgaattt
     6241 gcatggacca acttgctgtt gatattacag atatacctaa tgtagaagtt ggtaatacgg
     6301 caataataat aggaagagat aatttatctg aattatctgt ttctgaagtt gcaaataact
     6361 catgtagtat atcgaatgaa ttattgagca gagtaggaag aaggttaaat gttataaaga
     6421 agtaactcaa ttaaatagta atatttgttg tactgtgtaa tttattttac tctgactaga
     6481 catttttagt aatattatgt ctaatatata ttttttataa cttacatgac aatttaagtt
     6541 ataaaaaggt gatgtttcag atttttattt tgaaacatca ccttttttgt tataatatat
     6601 aataaaataa ttatacctta actgtaaact tatatagatt atttactttt taatattaga
     6661 ttcatttttt gagattggta tataataaac atatagagta tataataata gttaaagtta
     6721 aaaaagggga aaagcaatat gcagtatata caagaactac ttgatttgac tcaaaattat
     6781 tgggtgttag ccacaataat cggcttatta agtgcattta tagaaagctt tataccagcc
     6841 ttaccacttg ttgctatagt tacagcaaat gctgcaatac aaggattatt tattggatgt
     6901 ttgttatcat ggattggctc aggacttggg acaacttcat tattcttatt aataagcaga
     6961 tttaatgata gtaagctttt taataagttg agaaatagta aaactgaaaa agcaataagt
     7021 tggatggata aacagggatt taagttatta tttatagcgt atgcctgtcc atttatgccc
     7081 ggttgtttag ttactatcgc atcagctttt ggtaaaaaag atttaaaaga ttttgtgcca
     7141 gcaatgttgg caggaaaatt tgtaatgttt atagttataa gttacgtggc aagtgatata
     7201 gaaggtttta taacaagtcc tttaaagata gcatcattta tattattggt gtttttgtca
     7261 tggaagattg gaaatagagt aaataaaaat cttgaaaatc ataattatga tttccatcat
     7321 aaaaaacatc acaatgataa agatgataaa atgatttagc ttgtacgaaa taaaacgtat
     7381 cttaaaatga tttagtacta aattatttta agatacgttt tattttttgt attaaggtac
     7441 aaaattttta gttcttttgt aaactaaaat atataaaact tatttatact tagttcaaaa
     7501 tattgttatt tattttttct acaactataa taaaatataa aattgatatc caatcaaaaa
     7561 aataactatg aaatttaata gcttagatat gaatttttct gtttttgagt tagtttttat
     7621 aggtagtaaa gaaatgttta tagtaatggg ataaaacaat tcaatacctt ctttagtaat
     7681 aaggtcaaga cacaagtgag atagatatcc tattataaac ccactggaca aacatgagaa
     7741 aactatatta ttgttagtga agattgtcag agatttaaaa aaactataaa taagtaataa
     7801 ggcaattaga ctgtgagtaa accctctatg tctaaaccta cttccaaata atttgtatat
     7861 taacataaat tttttactta tgtaagaacc tctcatatct atatctggaa ataaagaacc
     7921 taaatatgca aaataaatat aaattataat taatactatt ttatatgcaa tatcgtattt
     7981 aactaggtac agattgttta taaaggggag tatcaaaagt gcgaatatat aacctcccct
     8041 ttcatgtgtt tcttttatca tataaaaatc ctcctgaatc gtcactataa acattatatt
     8101 ggtaaattta gactatcttt tattatatta tatagcaaac ataagttttt ttcaaaagga
     8161 ttttaatatt taattaatct tgagatttta aatttttgta attttctaaa gcaccattcc
     8221 aatttactac attaaaccag ttgtcaatgt attcatttct tctattttga tattttaaat
     8281 agtaagcatg ctcccaaaca tcaagtccta ttataggagt taggttttta cttacagggc
     8341 tatcctgatt tggagtagtc ataatagata atttcccatc tttagtagct acaagccaag
     8401 cccaaccaga accaaagaca tctaaagcag atttttggaa ctcttgctta aatttttcaa
     8461 aagaaccaaa gtctctatca atagcttctt ttaaagattc agaaggtatg gttttttctg
     8521 gcgtcattat atcaaaaaag aatttatgat tataagctcc acctgcatta tttcttacag
     8581 ttgtagcaat atctttaggt aaagaatcta aattttgcaa taattcacat aaagaataat
     8641 tataaagctc aggatatttt tcaagagcag catttaattt atcaacataa gcttgataat
     8701 gcttatcatg atgcagtttc attgtttctt tatctatata aggttcaagt gcatcatatg
     8761 cataaggtaa tggtttaacc ttaaatttgt tattttcagg tgtaaaagca aatgaagtta
     8821 tgcactgtga gattataaat aaagacataa taactggtat taatattttt ttcttcataa
     8881 aaatcacctc ctaatatagt atttctagaa ataaaaaaaa tatgaaatat taacagataa
     8941 tttataagaa tttaatagca atttcatttt aaattgaata agttttaaag ccaaagacaa
     9001 aactatgaaa tatagaataa gactaaagga cagaaaggaa ttttgattat atggacggca
     9061 atatttcaat ttattataaa ggtgatatat ctaaaaaaga agatattaaa tttaaaataa
     9121 aagaatctgt aaataataca agactttcta gtagaataga gtataaggat gaggaaaaaa
     9181 cttttgattt tgaaagattt aatgtaattg atgctattta tggaaataaa ccatttgaaa
     9241 aagatggaaa agttattgtt tttaatggaa atatatataa ctatcatgaa ataaaagaag
     9301 aattaattaa aaaaggacac aattttacta caaatgatga tattgaagtc ttattggcat
     9361 cttatataga atttggtaag aattgtgtac ataagattaa aggaatgttc aactttataa
     9421 tctatgatag agaaaatgaa agtatatttg gagcaagaga tttatttggg ataaaaccac
     9481 tctattatat aaataaggaa aatgcaataa ttttttcctc agagtataag tttttacttg
     9541 aatatatgaa aaatttaaat ataaatgaaa gaagcttaca aagttatttt tcatttcaat
     9601 atgtacttcc tgaagatact atgatacaag gaataaggct gataccagca ggtcactatt
     9661 ttagggtaga aaatggtata ctatctttaa agagatataa taaacttgag tttagaagta
     9721 gtacaaaatt tttttatact aaaaatcact taggcaatag agatgtgaac gaggatgata
     9781 taagaaatat agtagtagat tcaatagtaa ctcatatgga agaggagaaa gaaattggaa
     9841 catttctgtc tggtggaata gattcatcta ttataactac tgtggcttca caaattaatc
     9901 caaatataaa atctttttct gcaggcttta gtgtcaaagg atatagtgaa ttagaagtag
     9961 caaagaaaac agctgataag ttaggtatag aaaatatcca gattaatata acacaagatg
    10021 aatatataaa atcattaccg aatgtaattt attctttgga tgaccctata gcagaccctt
    10081 cagaagtagg tctttatttc ttaattaaag aggcaggtaa acatgttaaa gtagcattgt
    10141 ctggagaagg agcagatgaa ttatttggag gatataatat atataaagag tatagtacta
    10201 tgaaatcagt tgtcaattca cctacttata ttaaaagtat attaggaaga gtttcagaac
    10261 ttatgccaaa cataaaagga agaaattatc tttatagagc tacaacacca ttagaaaaaa
    10321 gatatatagg aaatgcaaaa gtttttgaaa acagcgaagt aaaaagattc ttttttaagt
    10381 ataaggaaaa gaatatatat gaatacctgt tgtctaattt gtatagagat gcacaaaaaa
    10441 ataattacga ttatataagt aaaatgcaac atatagatgt gaatacatgg cttcaaggcg
    10501 atatacttca aaaaattagc aaactttcta cagctgagca ggttgaactt agagtaccat
    10561 ttttatataa agatgtattt gatgtagcaa aaaatttaag aatggagcaa aaaataaata
    10621 agaataatac aaaagtttta cttagagagg cgtttagaga aatagtccca gaacatgttg
    10681 ttcaaaggaa aaaattagga tttccaactc caataagagt ttggttaaaa gattctctag
    10741 gggatattgt aaaggaaact atttataatt caaatgtaga tgaatttata gataaaaaat
    10801 atgcaataaa acttttagat acgcatctta aaggtcatag agataattcg agaaaaatat
    10861 ggactatatt tacattttgt ctatggcatc aattatttat tgagcataaa aatgtcgagt
    10921 attaatactt ttttcaatat taatcagagg gcaaaagcat gcaatgtttt caccctcctg
    10981 attattatgg aataggataa gtagaatttt gattgattag tttactatct caataaaatt
    11041 attggcaaat attttgacaa gattactttc aacagctaaa tttaagtttt gtccatcatc
    11101 atatccagcg gaaatcaatc ctagtaagtt tccaaacata tctaaaagag ctcctcctga
    11161 actaccacta gagattggag ctgtaaattg tatcatttga accgtttcaa aatcacgaaa
    11221 agctgaaata attccatctg aaactgtatt aaaaagacct agagggcttc caattgcaac
    11281 tattttttga cctctcacag gttttttact gactttaaca ggtattggct tgcattttct
    11341 atcaaccttt atcacagcta aatcataatc tgaatggtat tttataattt ggtaagatgt
    11401 ataaacttta tcatcatttt caaatctaac taaaaatgaa tacccatcat tgactacatg
    11461 aagattcgta agtatataac cttcattatt tattactact ccagaaccgc tcttaaacgg
    11521 tttattttcg ttattgcaca caacaatcat aacaacagaa gctgataact ttgcaaggtc
    11581 ttcataagaa agttctactt cctcatcttg tacaattttt ttagatgtta gatttgtgtt
    11641 attaagtgta tttttagagt taggtttatt cgtagtagga gaagtcttat tggtattttc
    11701 actgtaattt atatttgtaa tatgcctttc aaaaggtatt tctaatatat ttggagtctt
    11761 cctagaaagt tcaaatagag tagaaaattt tgaacattca ggatataaat caagaatatc
    11821 tttatttgga tttatatata aaatatcaca cccaagttgt gataaaaaat ataagaaaaa
    11881 aagttcttgc cttttaatat ttccaaagta tactatcttt ggtgaaatag tagtcttcac
    11941 cttgttataa aataatttag gtagaaaata gtctatccaa cacaaaagtt taactgcaaa
    12001 gtttttgatg atagaggatg tacaatttgg catacttgtg gaaaataaat ctaaagtatt
    12061 tttaaatgca atctttttag tccaatttaa ggtgtcattt tctattttac atggaaagtc
    12121 gatattatat aaatcccaac tgttaaactt agaactatta taaatattaa aaattttaag
    12181 catcttatct gtttgttctt tgtcaaaatt tatttttaga ttgtttaatc taatataggg
    12241 aattgtagga ttattaaaaa aataagtatc tatattttta attttttcta tgtatttatc
    12301 aattgaatca tcaacaccta tatatcttgt aaagtagact tctttaatat atgaagaacg
    12361 cttattttta taggtagtat cagatataaa ttcaattaga ggttcagtag acttttttat
    12421 tgaaatcttt aat
//
LOCUS       DAEPIN010000093        12216 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.093, whole genome shotgun sequence.
ACCESSION   DAEPIN010000093 DAEPIN010000000
VERSION     DAEPIN010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12216
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.093"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            complement(<1..684)
                     /locus_tag="KR250_003254"
     CDS             complement(<1..684)
                     /locus_tag="KR250_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069147.1"
                     /translation="MGEKKINNMNYSHNNYSKKTGEPTRKIKNSHITLKSKEEKICSS
                     CNKENNTKDNYCKFCGNELYEIASLRPLETKLDLKSKIKELSYHANKRGVFLTTFTTI
                     FILFIIALIFKAIITIQFNDISYLVNPAHIILALNLGQISVSMSTMMGSGFINANIGL
                     LILLIIPILVLLISNLIFMRKRCRDSKTVLANSLGVGIFYGLVLAILSIFTNVKTSSH
                     SMLEYGYALE"
     gene            1103..2278
                     /locus_tag="KR250_003255"
     CDS             1103..2278
                     /locus_tag="KR250_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBH0069148.1"
                     /translation="MNLEKIKKFFGFPLDKKHKDNVEKELCNYLYHTGKIICIIIVIS
                     QIIMMISISAREGGPFVTPRRQWYFYMYVTLFCVSSVFMFVFSYLWNKKKDCCTFYIN
                     LWIIYVTFFCLWGCVITLLDQLGGNDLSVFSYIMLAAAAFSVLKFGESVIVFGGCFLF
                     LNLFLPGIQTIPGNEFSNIVNTFFITSLAIFVSTMLYRNRVFTYYDKLLISQQYEEIS
                     HINNLLNDKVMTDDLTKMNNRRYLEDILQKKLISHANQEPITGMMIDIDYFKQFNDNY
                     GHQKGDYCLQNIAKVILDFTSSENEHAIRYGGEEFFICIFNCNEADAKLKSECLRKII
                     ESCNFERDDVPLKCVTVSIGVYTKKIGKTITLNEFISNCDKALYTAKEQGRNCVAFY"
     gene            complement(2451..3848)
                     /gene="asnS"
                     /locus_tag="KR250_003256"
     CDS             complement(2451..3848)
                     /gene="asnS"
                     /locus_tag="KR250_003256"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HBH0069149.1"
                     /translation="MQKEFITVKELYREKEQYIGKTVKVAGWIRTSRVSKNFGFIELN
                     DGSFFKNMQIVFDEKVENFKEVGKLAISSSILVEGELVSTEGAKQPVEIHAKSIVVEG
                     ESDSSYPLQKKRHTLEYLRTIAHLRPRSNTFSAVFRVRSIAAYAIHKFFQERNFVYAH
                     SPIITGSDCEGAGEMFRITTMDLNDIPKTEDGKIDYTQDFFGKEANLTVSGQLNAEIM
                     ALSFRNVYTFGPTFRAENSYTGRHASEFWMIEPEIVFADLEDNMELAEDMIKYVISYV
                     LENAPEEMEFFNSFIDKGLLERLNKIVNSEFIRITYTKAVELLIESGHEFEYPVEWGC
                     DLQTEHERYITEQIYNCPVFVTDYPKDIKAFYMRMNEDGKTVRAMDLLVPGVGEIVGG
                     SQREEREDVLLDRINEMGLREEDYWWYLELRKFGTATHSGFGLGFERIIMYMTGMSNI
                     RDVIPFPRTPKNAEF"
     misc_binding    complement(3925..4158)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(4327..4521)
                     /locus_tag="KR250_003257"
     CDS             complement(4327..4521)
                     /locus_tag="KR250_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3787 domain-containing protein"
                     /protein_id="HBH0069150.1"
                     /translation="MESKKARALKGKDNKRLKSSLLVGNESTAAWADVEKLKPHSKVS
                     IPSIKGVEEAKDWVDNGSKL"
     gene            complement(4794..6467)
                     /locus_tag="KR250_003258"
     CDS             complement(4794..6467)
                     /locus_tag="KR250_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HBH0069151.1"
                     /translation="MEKSYCIIYQGDIESALQENGINRYMVLNSQLAVIYVPLDFDET
                     ILNNIIQVAWWEESEPMSSLIEITNNVNNGETITTAAETDYIYENPYNDITGRGILLA
                     VIDSGIDYLHPDFINDDGTSKVLYLWDQEANTNPPPEGFIFGSEFTRSELNIAINRND
                     GSLSQDNIGTGTLVSGILVGNGRINSQYRGITTESDLIVVKLKSYTDTYYAGRINYSV
                     SDFLAAITYVTNIARTENKPLIINLTIGVKSSAVATTSILDTFNILSSAGVVVVSGAG
                     NQGNTDIHYSGRFSSVGEVQDVIIQDGDDYALDITLNTNGPDKVGAQIISPSGEVSHD
                     IRYSPDFYIYRGKFNLENTTYAMRFIYPYITSGKENLEIRLRDIKPGVWILRLTSELI
                     INGEYDIYLPNKNLIAPDTRFLDPDSVATITMYAASDDVITVGTFNNKTDSMWIGSSK
                     GPIRGRGIKPDIVASGVDIISTYKNGTYNTGTGTGVSSSIVTGVLALLMEYLEKQDNV
                     PRLSLFTQVLKTYLILGATKLEIYTYPNVSQGYGILNLKNTIQQIANTL"
     gene            complement(6477..9875)
                     /locus_tag="KR250_003259"
     CDS             complement(6477..9875)
                     /locus_tag="KR250_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HBH0069152.1"
                     /translation="MIIINYELIVKYNGDILRLEEELGVSVEILNSSYAIITSSNEED
                     VNILLTYPEIEFIEKPFILQTQDVQSFSSTGITGFKNRTGLTGKGTIIGIIDSGIDYT
                     LPVFRDSDGRSKILYYWDQSIQGNPPEGFREGTLYTNEDINNAIDGSMYIPISTTSLH
                     GTHVAGICATIASDARIIVVRVGNIQTDIFSRSTEFMRAIKFILDRALELRMPVTLNI
                     SYGSNEGSHRGTSLFEQYIDDMCLFWKNNIVVAAGNNADKGGHKRIRLQNNITEEVEF
                     IVGEGERILNINIWPDFVDDFSVHLVNPSNNQTQAISLTSGEIRNTLGETRITGYFYP
                     IAPYSLTRRVTLQLSSNTQITPGLWKIVFEPIDIVTGNVNIYLPTSEGLNRNTRFLIP
                     TQELTVTVPGTASRVITVGSFNSRTDIVSIFSGEGDTQLGVFKPDLLAPGEDIVSFLP
                     GGTSGALTGTSMATPHVTGVCSLFMEWGIVNGNDLFLYSQKLRALLLKGARRLSNQSY
                     PNNSSGFGFLNLSDIDLYTLSNINQDLETEDIGYRSINKSFKDEENSYKFIDGYNMQI
                     HNDLENEIYISKNASRQSGILSGIDIVHTPEFEEELAGLGMSQRFFKISDSLGVLSIN
                     NTDYNSIQRVLQLPSIIRTVSTTKMTLLGEINRGTFGGVVATEEMGVNFFKNNPNINI
                     TGRGTLISIADTGIDYLHPDFIYPDGTSKIVYLWDQTKEGTPPDGFYIGTEYTREDIN
                     RAIAENDPSLSQDEVGQGTMLSGICAGLGNVNSEYAGIAEDSELIIIKLGKIDDFYNS
                     AMLFAASQYAYKKAFELGRPLVINMSLGTSSLAGLTNRSNSEKAFFTRGLCITAGAGN
                     EGNTQTHTSGIIPYVGGSVEVELELNEDEEELSLELWLNRPDKADVIIVSPTGEESKS
                     VGISNYNKVTGLFDLEGTEYSITYIYPTTFSGQQFTNVTLKNAKRGVWKIRLVGVYII
                     TGRYNLYLPNRELLKSGTRFREVDPFYTINYPAIQDDLITIGAYNTINGSLWQSSSRG
                     PTIEDRLKPDIVAPGVNIIAAYPGNTYATITGTAAASAHAAGAAAMYFQYTFVDGRYP
                     NQAYVQKIKTFMQAGARKDSNTVYPNTNSGYGLLDVRGMFDVLR"
     gene            complement(10042..11238)
                     /locus_tag="KR250_003260"
     CDS             complement(10042..11238)
                     /locus_tag="KR250_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HBH0069153.1"
                     /translation="MFIEFFYLLRSRGLNVSLNEWMTLIEALDKGLCYSNFSNFYYLC
                     RMILIKSESDFDKFDAVFLEYFKGIEHQEEIPEEIMNWLKKPDIDLEEFERLEQNPNI
                     NLDKLRAKLEERIKEQDSPHNGGNYWIGTGGTSELGHSGKGQTGIRIGGNSTYGRAVL
                     EVAGERKYRDFRDDEVLNMRQFQVALKRLRQFSTRIDAPKTELDLDKTIEETCNNAGY
                     LKLFFEKPRKNTVKLLLLMDSGGSMRGYSTLCNTLFQSVSKSNHFKDVKIYYFHNCIY
                     DRLFTTPECWLSKSINTEWILKNIDKNYKVIIVGDASMSPSELLHIGGNYRGPYNYTP
                     GIEWLKRFKRKYSKIVWMNPELRDGWDSINYWYQTQRMIQSEFNMFPLTVKGLEKALK
                     NLMVSR"
     gene            complement(11259..12080)
                     /locus_tag="KR250_003261"
     CDS             complement(11259..12080)
                     /locus_tag="KR250_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HBH0069154.1"
                     /translation="MIKKEISNFRGSSDYVVSPELMASVNVAIALEKPLLIKGEPGTG
                     KTMLAQAISNELKKDLVIWNIKSTTKAQEGLYVYDTVQRLYDSQFGGEGVDDISKYIK
                     YGKLGEAFSSNQQVILLIDEIDKADLEFPNDLLWELDKMEFYINETKETVRAKQRPIV
                     IITSNAEKELPDAFLRRCIFHYIEFPDRDMMEEIVKVHFDKVEEHLLEQVMTTFYWIR
                     SLKDIQKKPSTSELIDWIQALTLSGMPIEKIEKEVPFAGILLKNNEDIESMQRHL"
BASE COUNT         3920 a         2186 c         1402 g         4708 t
ORIGIN      
        1 ttctaatgca tatccatatt ctaacatact atgtgaacta gtttttacat tagtaaaaat
       61 agataaaata gctaaaacta atccataaaa aattccaact cctaaagaat ttgctaatac
      121 agtttttgaa tctctacatc tttttctcat aaaaattagg tttgatatta ataataccaa
      181 aatagggatt ataagtaata tcaacaaacc tatattggca tttataaacc cagaacccat
      241 catggtagac atagatacac taatttgacc caagttgaga gctaggatta tatgtgctgg
      301 atttactaaa taactaatat cattaaattg aatagttata atagctttaa atatcaatgc
      361 aataataaac aatataaata tggttgtaaa tgttgttaaa aatacacctc ttttatttgc
      421 atgatatgaa agctctttta ttttagattt caagtctagt tttgtttcaa gtggtcttaa
      481 tgatgctatt tcataaagtt catttccaca aaatttacag taattatcct tagtattatt
      541 ttctttattg caactagaac aaattttttc ttctttactt ttaagagtga tatggctatt
      601 ttttattttt ctagttggtt cacctgtctt tttggaataa ttattatgtg aataattcat
      661 attatttatt ttcttttcac ccattttttc cctccaagtt ttactattgt aaattatcaa
      721 atataactat atcatataaa acttaattat accttaattg gatatattta tctattttat
      781 ttgtttattg ctgttgtttt ttaacaggtc ttgatattta aatagtttgt taatgcgaac
      841 tttattattt atattttatt atgcttaatt taaatttatt ctattcttta ttttcagtag
      901 taaactatct gtaaatctta attataataa agtattaatc aataaagtta ctcgacatta
      961 tctaataaat tttatctatc acaattttag attgttacac acaatatatt ttgatataat
     1021 taaatcataa acgaagtatt ccaaatatct aatgatagaa aggaatactt atttttatgc
     1081 tttattttta atggaggctt atatgaactt agaaaaaata aaaaaatttt ttggatttcc
     1141 tctagacaaa aaacacaaag acaatgttga gaaagaactc tgcaattatc tttatcatac
     1201 gggtaaaatc atttgcataa taatagtaat atctcaaata attatgatga tatcaatctc
     1261 agctagagaa ggaggccctt ttgtcacacc aagacgccaa tggtattttt atatgtatgt
     1321 aacactattt tgtgtatctt ctgtctttat gtttgtattt tcttaccttt ggaataaaaa
     1381 aaaggattgc tgtacgtttt atataaattt atggataatt tatgtgactt ttttctgcct
     1441 atggggttgt gtaattactc tacttgacca gttaggtgga aatgacctaa gtgtatttag
     1501 ttatattatg ttagcagctg cagctttctc agtattaaaa tttggagaat cagtaatagt
     1561 ttttggagga tgctttttgt ttcttaattt attcttacct gggattcaaa ctataccagg
     1621 caatgagttt agtaatattg tcaacacatt ttttataaca tcccttgcca tttttgtttc
     1681 tacaatgcta tatagaaatc gtgtatttac ttactatgat aaacttttaa ttagtcaaca
     1741 atatgaagaa atatctcata taaataattt acttaacgac aaagttatga cagatgacct
     1801 tactaaaatg aacaaccgta gataccttga agatattcta caaaaaaaat taatcagcca
     1861 tgcaaatcaa gaacctataa ctggaatgat gatagatata gattatttta aacaatttaa
     1921 tgataattat ggacatcaaa agggagacta ttgtctacaa aatattgcca aagtaatttt
     1981 agattttaca agttctgaaa atgaacatgc tatacgatat ggtggtgagg aattttttat
     2041 atgtattttt aactgtaatg aggcagatgc taaacttaaa tcagaatgtt tacgaaaaat
     2101 tatagagtcc tgcaattttg agcgtgatga tgttccttta aaatgtgtaa ctgttagtat
     2161 tggagtttat acaaagaaaa ttggaaagac aataacatta aatgagttta taagtaattg
     2221 tgataaggct ctatatactg caaaagaaca gggacgtaat tgtgttgcat tttattaaaa
     2281 ggttacatac tattacataa acctataagt attatatcgc aaaataaaag taatcaaatt
     2341 atatatttat agtacaataa aaataacttg catatctcta aaatagataa atatgcaagt
     2401 cattttagtg tttatactta aagttatata agttttatta tatataatta ttaaaattca
     2461 gcatttttag gagttcttgg gaaaggtata acgtctctta tgttagacat acctgtcata
     2521 tacataatta ttctttcaaa tccaagacca aatccagagt gagtagcagt accaaacttt
     2581 ctaagttcta aataccacca ataatcttct tctcttaatc ccatttcatt aattctatct
     2641 aacaatacat cttctctttc ttctctttga gaacctccta cgatttctcc aactccagga
     2701 actagtaaat ccattgctct aacagtttta ccatcttcat tcattctcat atagaaagct
     2761 tttatatctt ttggataatc tgtaacaaac actggacaat tatatatttg ctccgttata
     2821 tatctttcat gctcagtttg aaggtcacat ccccactcaa caggatactc aaattcatgc
     2881 ccagattcaa ttaataattc cactgcttta gtatacgtta ttctaataaa ctcagagttt
     2941 actatcttat ttaacctttc taacaatcct ttatctataa aactattaaa aaattccatt
     3001 tcttcaggag cattttctaa tacatagctt ataacatatt ttatcatatc ttctgcaagt
     3061 tccatgttat cttcaaggtc tgcaaaaact atttctggct ctatcatcca aaattctgaa
     3121 gcatgtcttc ctgtataaga gttttcagct ctaaatgtag gtccaaaagt ataaacgttt
     3181 ctaaaagaaa gtgccattat ctcggcattt aattgaccac taactgttaa attagcctct
     3241 tttccaaaga aatcttgagt atagtctatt ttgccatctt cagtttttgg aatatcattt
     3301 aaatccatag ttgttattct aaacatctct cctgcacctt cacagtcact acctgttatg
     3361 attggagaat gtgcatatac aaagtttctt tcttggaaga atttgtgtat tgcataggca
     3421 gctattgatc ttactctaaa tacagctgaa aaagtattac ttcttggtct taaatgtgct
     3481 atcgttctta aatattcaag cgtatgtctc ttcttttgta gtggatatga actatctgat
     3541 tccccttcta caactatgct ttttgcatgt atttcgactg gttgtttagc cccttctgta
     3601 gatactaact ctccctctac aagtatagat gagcttattg ctaattttcc tacctcttta
     3661 aaattttcta ctttctcatc aaatactatc tgcatatttt taaagaagct tccatcattt
     3721 agctctataa atccaaaatt tttagatact ctagatgttc ttatccatcc agctactttt
     3781 acagtcttcc ctatgtactg ttctttttct ctgtacaatt cttttactgt tataaattcc
     3841 ttttgcattt taaatcctcc tagtataatt gttattaatt tcttttaaat ataatttcaa
     3901 taaaaaaagc ccttcatccc tatataaagg gacgaaagac tattactctc gcggtaccac
     3961 cctagttgtc ataatgacct cttagttaag caatgcttaa agcctataac ggtgccttcc
     4021 gtctaagtct acttactaaa taagctttcg attagaaact ccaagatgtt cttcaatata
     4081 aacctcgtat tgagctttca ccatcctcaa ctcgctagaa ctacatttta tatctactct
     4141 tctcttcaca gtatttatct tttattattt ctgctaatta acaaaattat accaatttat
     4201 aaatttttag tcaataaatt tatataattg atactgattg ttatttaaaa tataataatt
     4261 ctaccttaca taatgataat aaatacttat cttcattact tctttattac ttcaatttcc
     4321 aaaattttat aatttacttc cattgtcaac ccagtctttt gcttcttcga cacccttaat
     4381 tgaaggtatt gaaactttac tatgaggttt taatttttca acatcagccc atgctgctgt
     4441 cgactcattt ccaactaaaa gtgaactttt aagtctttta ttatctttac cttttaatgc
     4501 tcttgccttt ttactttcca aaactatcac ctctattatt tattttgtaa tagttttagc
     4561 aattttacaa ataaaattca taagtctaaa ttataattat ttttaaatta acatcatatc
     4621 ttttcaatac ttaatttata cctatttata tacaaaatat ttactttgtg tattcaaaat
     4681 ataaaactgt tttttaattt tcatatttat tttttatgct atttttctac tctatctaaa
     4741 gtatttgata caattaaact aataatcaat tataatttta cataggttct tatctataga
     4801 gtatttgcta tctgttgaat cgtatttttt aaatttaaaa tcccatatcc ctgcgataca
     4861 tttggatatg tatatatctc aagttttgta gcacccaata ttaaatatgt ttttaaaact
     4921 tgtgtaaaca atgataatct cggtacatta tcctgttttt ctaagtattc catcaataag
     4981 gctaaaacac ctgtgactat agaactgctc actcctgttc ctgttcctgt attatatgtt
     5041 ccatttttat atgtggatat tatatctaca cctgaagcta ctatatctgg cttaattcct
     5101 cttcctctaa ttggtccttt cgaagaacca atccacatac tatcagtctt attgttaaat
     5161 gtaccaactg taattacatc atcactagca gcatacatag ttatagtagc tactgaatca
     5221 gggtctaaaa accttgtgtc tggagctatc aaatttttat ttggcaagta tatgtcatat
     5281 tctccattta ttataagttc tgaagtcaat cttaaaatcc atactccagg ttttatatct
     5341 cttaatctta tctccaaatt ttcttttcca gaggtaatat atggataaat aaatctcatt
     5401 gcataagtag tattttctaa gttaaatttt cctctatata tataaaagtc tggagaatac
     5461 cttatatcat gactaacttc acctgaagga gatattatct gtgctcccac tttatctgga
     5521 ccattcgtat ttagtgttat atcaagagca tagtcatctc catcttgtat tattacatct
     5581 tgaacttctc ccacactgct aaatctgcca gaataatgaa tatctgtatt cccttgattt
     5641 cctgcaccac tcactactac cacaccagca ctactcaata tattaaaagt atctagtatt
     5701 gaagtagttg ctacagcact tgacttaact ccaatagtca aatttattat cagtggttta
     5761 ttttctgttc ttgctatgtt agtaacatac gttatagctg ctaaaaaatc tgaaacactg
     5821 taatttatcc ttccagcata ataagtatct gtatatgatt ttaatttaac tactattaag
     5881 tctgattctg tagtaatacc tctgtactga gaatttatcc ttccatttcc aactaatata
     5941 ccagaaacta aagttccagt acctatatta tcttgactca aactcccatc atttctattt
     6001 atggctatat ttaactcact tcttgtaaat tcacttccaa aaataaaacc ttctggaggt
     6061 ggatttgtat ttgcttcttg gtcccataaa tataaaactt tactagttcc atcatcattt
     6121 ataaaatcag gatgcaaata atctatccca gaatctataa ctgctaataa aattcctctc
     6181 cctgttatat cattatatgg attttcataa atataatcag tttcagctgc tgttgtaatg
     6241 gtttctccat tatttacatt attagttatt tcaattagac tgctcattgg ttcagattct
     6301 tcccaccaag caacttgtat tatattattt aaaatagttt catcaaaatc taaaggtaca
     6361 tatattactg caagctgact atttaaaacc atatacctat ttattccatt ttcttgtagg
     6421 gcagattcta tatcaccttg ataaattata caataagatt tttccataaa tccctcctat
     6481 cttaaaacat caaacattcc tctaacatcc aagagcccat atccagaatt tgtatttgga
     6541 tatactgtgt ttgaatcttt tctagcacca gcttgcataa aagtttttat tttttgcaca
     6601 tatgcttggt ttggatatct accgtcaaca aaagtatatt gaaaatacat tgcagcagca
     6661 ccagctgcat gtgcacttgc tgctgctgta ccagttatag ttgcatacgt gttcccagga
     6721 taagcagcta ttatattaac acctggtgct actatatctg gttttaacct gtcttcaata
     6781 gttggccctc ttgatgaact ttgccataaa ctaccattaa tagtattgta ggctcctatg
     6841 gttattaaat catcttgaat cgcaggataa tttatagtgt aaaaagggtc tacctctcta
     6901 aatctagttc cactttttaa aagttctcta tttggaaggt ataagttata tcttcctgtt
     6961 ataatataaa ctcctactaa tcgaatcttc catacacctc tttttgcatt ctttaaagta
     7021 acattagtaa attgctgtcc agaaaaagtt gttgggtata tatatgttat agaatattca
     7081 gtcccctcta aatcaaataa acctgttact ttattataat ttgaaattcc aacactttta
     7141 ctttcttcac cagtgggaga tactattatt acatcagctt tatctggtct atttaaccaa
     7201 agttctaatg ataattcttc ctcatcttca tttaactcta attcaacttc aacagaacct
     7261 ccaacatatg gtattattcc agatgtatgt gtctgtgtat tgccctcatt tcctgctcct
     7321 gccgttatac ataaccctct agtaaagaac gctttttcac tattactcct attggttaaa
     7381 cctgctaggc tactggtacc taaagacata tttataacta atggtcttcc aagttcaaaa
     7441 gcttttttat atgcatactg agatgcagca aacagcattg cactattata aaaatcatct
     7501 atcttgccga gttttattat tattagctca gaatcttctg ctatacctgc atattcacta
     7561 tttacatttc caagccctgc acatatccca ctcaacatag ttccttgacc aacttcatcc
     7621 tgagatagac tagggtcatt ttctgcgata gctcgattta tatcttctcg tgtatattct
     7681 gtcccaatat aaaaaccatc aggaggagtt ccttcttttg tttggtccca taaatatact
     7741 atttttgaag tgccatctgg ataaataaaa tctgggtgta gatagtctat tcctgtatct
     7801 gcaattgata ttagagtccc tcttcctgtt atattaatat taggattatt tttaaagaaa
     7861 tttacgccca tttcttctgt tgcaactaca cctccaaaag tacctcgatt aatttctcca
     7921 agtaatgtca tctttgtagt cgatacagtt cttatgattg aaggaagttg caaaactcgt
     7981 tgtatgctat tatagtctgt attatttata gatagtacac ctaatgaatc acttatctta
     8041 aaaaaccttt gactcatacc taacccagct agttcttctt caaattcagg agtatggact
     8101 atatctattc ctgatagtat tcctgattgt ctacttgcat ttttacttat ataaatttca
     8161 ttctctaagt cattatgtat ttgcatatta taaccatcta taaacttata actattttcc
     8221 tcatctttaa atgacttatt aatacttcta tatccaatat cctctgtttc taaatcttga
     8281 tttatattag atagagtgta caaatctata tctgataaat ttaaaaaacc aaatcctgat
     8341 gaattatttg gataagattg attacttaat ctccttgctc cctttagaag taatgccctt
     8401 aatttctgtg aatacaaaaa taaatcattt ccatttacaa ttccccattc cataaacaat
     8461 gaacaaaccc ctgtaacatg aggtgtagcc atgctagttc cagttaatgc tccacttgta
     8521 cctcctggta aaaaagatac tatatcttca cctggagcta ataaatcagg tttaaaaaca
     8581 cctaattgtg tatctccttc accagaaaat atagatacta tatctgttct tgaattaaaa
     8641 ctacctactg ttataactct acttgctgtt ccaggaactg ttacagtaag ctcttgagta
     8701 ggaatcagaa atctagtatt tctatttaga ccttcagaag ttggcaaata tatatttaca
     8761 ttaccagtta ctatatctat tggttcaaat acaattttcc ataacccagg agttatctgt
     8821 gtatttgaac ttaattgtag agtaactctt cttgtgagag aatacggtgc tatagggtaa
     8881 aaataaccag ttattctagt ttcacccaaa gtattcctta tctcacctga agttagagag
     8941 attgcttgtg tttgattatt tgatgggttt accaaatgta cactaaaatc atcaacaaaa
     9001 tctggccata tatttatgtt taatatacgc tctccttccc caactataaa ctcaacttct
     9061 tctgtaatat tattttgaag cctaatcctt ttatgacctc ctttatctgc attatttcca
     9121 gctgctacaa ctatattgtt tttccaaaat aaacacatat catctatata ttgttcaaat
     9181 aaagatgttc ctctatgtga accttcatta cttccataac ttatatttaa tgtaacaggc
     9241 attcttaatt ctaatgccct atctaaaata aatttaattg ccctcataaa ctctgtactt
     9301 ctcgaaaaaa tatcggtttg tatatttcca actctaacca ctattattct ggcatcactt
     9361 gcaatagttg cacaaatacc tgcaacatgt gtcccatgta agctagttgt tgaaataggt
     9421 atatacatac ttccatctat tgcattattt atatcttcat tagtatataa tgttccttct
     9481 ctaaaacctt ctggtggatt tccttgaata gattggtccc aataatataa tatcttagac
     9541 cttccatcac tatctctaaa cacaggtaga gtataatcta ttcccgaatc aattatacca
     9601 attatagttc cttttccagt taaaccagtt ctatttttaa atcctgttat tcctgtactt
     9661 gaaaaacttt gcacatcctg agtctgtaat ataaaaggct tttctataaa ttcaatttct
     9721 ggataagtta aaagtatatt tacatcttct tcattacttg atgttattat tgcatatgat
     9781 gaattaagta tctctactga tacacctaat tcttcttcta acctcaatat atctccattg
     9841 tattttacaa ttaattcata atttattata atcacctcga cttctaaaat tattattaat
     9901 gtatatgttt tgtgtgagtc taaaatgtat tagactatat aattttttaa gtttcagtta
     9961 tacaatttat aataggtata ttaaaaaaat aaataatgat ggtacaattt tgaataaatc
    10021 ataccatcat ttatatttac tttatcttga aaccattaaa tttttcaatg ctttctcaag
    10081 accttttaca gtcaatggaa acatattaaa ttcactttgt atcattcttt gagtttgata
    10141 ccaataatta attgaatccc atccatctct aagttctgga ttcatccata caattttact
    10201 gtactttctc ttaaaccttt taagccactc tataccaggt gtgtaattat atggaccacg
    10261 atagttacca ccaatatgaa gcaactcaga aggcgacata gaagcatctc caacaattat
    10321 aactttatag tttttatcta tattttttaa tatccattca gtattaatag atttacttaa
    10381 ccaacattca ggtgttgtaa ataatctatc ataaatacag ttatggaaat aatatatctt
    10441 tacatcttta aaatgatttg atttacttac agactgaaat aatgtattac ataaagtgct
    10501 ataccctctc attgaaccac ctgaatccat aagcaataaa agcttcactg tattttttct
    10561 tggcttctca aaaaataact ttaaatatcc tgcattatta caagtctctt caatagtctt
    10621 atccaaatca agctctgtct ttggagcatc tattctagta gaaaattgac gaagtctctt
    10681 tagtgcaact tgaaattgac gcatatttaa aacttcatca tctctaaaat ctctatattt
    10741 tctttctcct gcaacttcaa gaacagctct accataagta gaatttccac ctatacgtat
    10801 accagtttgc ccttttcctg aatgtcctaa ctctgaagtg cctcctgtgc ctatccaata
    10861 attaccacca ttatgaggag aatcttgttc ttttattcgt tcctccaatt tagctcttaa
    10921 tttatcaaga tttatatttg gattttgttc taatctttca aactcttcta aatctatatc
    10981 tggtttttta agccaattca taatctcttc tggaatttct tcttggtgtt ctataccttt
    11041 aaaatactcc aaaaacactg catcaaactt atcaaaatca ctttcacttt ttattaaaat
    11101 catcctgcaa agatagtaaa aatttgaaaa gtttgaatag cacagtcctt tatccaatgc
    11161 ttcaattaaa gtcatccatt catttaaaga tacatttaat cctcttgacc tcaataaata
    11221 aaaaaattca ataaacataa ttatccagct cctattaact ataagtgtct ttgcatagat
    11281 tcaatatcct cattattctt aagtagtatt cctgcaaaag gaacttcttt ttctattttt
    11341 tcaataggca ttccactcaa agttaatgct tgtatccagt ctattaactc tgatgtacta
    11401 ggcttttttt gtatgtcctt taaacttcta atccaataaa aagtagtcat tacttgttcc
    11461 aataggtgtt cttctacttt atcaaaatgt actttaacta tttcttccat catgtctcta
    11521 tcaggaaatt ctatataatg aaatatgcat cttcttaaaa aagcatctgg aagttctttt
    11581 tccgcatttg aagttataat aactattggt ctttgtttag ctcttacagt ttcttttgtc
    11641 tcatttatgt aaaactccat tttatccaat tcccaaagca agtcatttgg aaattctaaa
    11701 tccgccttat ctatttcatc tattaaaaga attacttgtt gatttgagct aaaggcctct
    11761 cctagtttac catattttat gtacttgcta atatcatcta caccttctcc tccaaattgg
    11821 ctatcataaa gtctttgaac agtatcatat acataaagcc cttcttgtgc ttttgtagtt
    11881 gattttatat tccaaattac taaatctttt tttaattcat ttgaaattgc ttgtgctagc
    11941 atagttttac ctgttccagg ttcgccctta atcaaaagtg gcttttcaag agcaatagca
    12001 acattaactg atgccatcaa ttctggtgat actacataat cactacttcc tctaaagtta
    12061 gatatctctt tttttatcat aaatcctact ccttttaaac agtattttct aaatgtatat
    12121 agactttatt actatttttg atataaaaat aataatacaa tacatttgta tagtattatt
    12181 ctaaaattct atatctaaat aaaatcacct ctaaaa
//
LOCUS       DAEPIN010000094        12049 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.094, whole genome shotgun sequence.
ACCESSION   DAEPIN010000094 DAEPIN010000000
VERSION     DAEPIN010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12049
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.094"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            1..877
                     /locus_tag="KR250_003262"
                     /pseudo
     CDS             1..877
                     /locus_tag="KR250_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432854.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
     gene            1068..1235
                     /locus_tag="KR250_003263"
                     /pseudo
     CDS             1068..1235
                     /locus_tag="KR250_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418025.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            complement(1495..4062)
                     /locus_tag="KR250_003264"
     CDS             complement(1495..4062)
                     /locus_tag="KR250_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002602373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HBH0069155.1"
                     /translation="MIYYVLPLEHKQATIEIVGGKGMSLSKLLTAGIPVPDGFHVTTA
                     SYQIFVETNHIQSRINKLLDGIDSNNTSQLEDVSKKIGELFHNGEMPQEVSDAIKMAY
                     AGLGNISVAVRSSATAEDLPDASFAGQQETYLNIQGEDKVIDSVKRCWASLWTARAIA
                     YRVKNNIKHEIVALAVVVQKLAFSDSSGIMFTLNPINGRRSEMIINAAWGLGEAVVSS
                     LVTPDTIVVDKDSERIISYEVANKEIMTVRTSEGTEETMVPERLRKKYALTRNQVMQL
                     IQLGKKIEKYYQMPMDVEWALEKDKLYIVQARPITVLPPEWVLPEQDVVYTKGSLAEH
                     LPNPVTPLFATLGLEIVNRASELLWIDMFGKSAKKLLPKNGAYTVINGYVYLSAKSNP
                     LLIAIKSLSPRSLRRTLMNSVARWETARKGYEDAIKQWEEKPLHTMNAHQIMEGIQTI
                     FYAACIYFTRIQLTLPAASISETLFTKLFQGAVRRAGITDTSVFLLGFDTIVLQSEKE
                     LWGISEWVKQNNSINLYLQNSPAIKIAEDFMSSVVPVGVSQEVWIEWKNRINKYFKEF
                     GRTAYEFDFAHSTPQETLTPTFESVKTFVEGKGESPFLRQTTFEKRRKHAEEEILQHI
                     GGLRKKLFFKLLHWAQETSPMRENAIYLMGMGHPLIRCMFQEISERFIRGGAISHLDD
                     IYWLTKSELEVLIEQLDKNKSLSDMNGIIPVRKAELKKYMGYVSPSKLPEKNKKTISQ
                     AQQKQKDGKIVLTGIGTSTGVVTAPACVLNSPADFESFQPGSVLVAVTTTPAWTPLFS
                     SASAIVTDIGGPLSHSSIVAREYGIPAVMATHIATRAIKSGQMITVDGLAGTVTFDE"
     gene            complement(4065..4670)
                     /locus_tag="KR250_003265"
     CDS             complement(4065..4670)
                     /locus_tag="KR250_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002591427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBH0069156.1"
                     /translation="MPKGIVLTPEQQAERREKIVAVALSLMAENGFQKTSMREIAVLA
                     NMGKSSLYDFFKTKDEIVVYAVEKEIEKTIQQVHRIIDGEPSPQQCLRKIMLNHLRFP
                     EQYRTVLMWLNTESDYLEEEYRKRLKDARYAYQDIIKSVIDNGVTTGIFRKTDTALVT
                     RLLINSMLSISYTSRPLDSSEKMLDETMNIFLHGIMNDGGE"
     gene            complement(5044..6504)
                     /locus_tag="KR250_003266"
     CDS             complement(5044..6504)
                     /locus_tag="KR250_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HBH0069157.1"
                     /translation="MFNELKTKENVYKNLINGKWVESNSRKPIEIYSPIDNSLVGKVQ
                     SMTKHEVDEVIKNTKESIKVWAEMPVYKRANIFHKAADLLLENIDEIANILVLEIAKD
                     IKSARAEVERTADFLRYTADVGKNMEGEAISGDNFPGGTRNKMSYVSRVPLGTVLAIS
                     PFNYPVNLSMSKIAPALIGGNAVVLKPATQGAISALHVVEIMRKAGIPDGVLNTITGR
                     GSEIGDYVVTHKGINFINFTGSTEVGQHISKISGMVPLLLELGGKDAAIVLEDADLDF
                     AAKNIVSGAYSYSGQRCTAVKRILVQESVADKLVGKIKPLVEKLTIGNPMDEVVITPL
                     IDNKATDFVQGLVDDALHKGAKLITGNVRKNNLFYPTLLDNVNVDMKIAWEEPFGPVL
                     PIIRVKDINQAIEIANQSEYGLQSSVFTSDIDKAFYIADKLEVGTVQINNKTERGPDH
                     FPFLGVKASGMGTQGVKYSIEAMTRPKAVVVNVREL"
     gene            6858..7436
                     /locus_tag="KR250_003267"
     CDS             6858..7436
                     /locus_tag="KR250_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBH0069158.1"
                     /translation="MAKSFTEIEKDNIRKRLISECEINWSKYGYKKTNIDELCSKSGI
                     AKGSFYTFFDSKEKLFFEVLNGIQNRLISSVDEILSNMPPKEGFAKALKMLYRLYDKN
                     PFLYSPNNTDYITLLNKIPKEMLSQLEYSNVQSFYSLIERHNLKLKIEKEKAFGVCNA
                     LLSSLLLKEDIGYDYIEVFDFMVDNLVEHILE"
     gene            7487..7720
                     /locus_tag="KR250_003268"
     CDS             7487..7720
                     /locus_tag="KR250_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069159.1"
                     /translation="MIELIQRLNDIIYKINDKLNIHTKLVDPSSELMPLYSMLSIIVS
                     IFLIILGIKVRKKFLIIIAIISIIINILIILDR"
     gene            7729..8979
                     /locus_tag="KR250_003269"
     CDS             7729..8979
                     /locus_tag="KR250_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBH0069160.1"
                     /translation="MVKSSNTFNKNFNLMILGQIISLFGASILKFALSLYILDITGKA
                     EIFATILAVSSIPIIVFSPIGGAIADIFNRRNLMVIFDFSSSLTVLILAVFLFNNNGS
                     ILLVGIIMTILSVISTMYQPTVQSSTPLLVDNEHLMNANGIVAGVASLTNIAGPVLGG
                     VLYGVIGINAIIVISCVSFFLSAIMEIFIQIPFIKQEQNSNIIKTIFTDIKDGIVHIC
                     KENRFILKILFLAIAINLFLSSMLIVGIPYIVKITMGASNFLYGLAEGAISFSSLVAA
                     VSIGIFSKHLKITNLHISFIICAVMFIPMAVSVYPFILNTGFPVPFLIFIVSSMLIFF
                     TTTLISIYVITVTQLKTPNELLGKVMSILFAGTAIAMPLGQVMYGKLFDLLSDRVYII
                     ILGVGFITLLIAFVSKITLRNENL"
     gene            9140..9709
                     /locus_tag="KR250_003270"
     CDS             9140..9709
                     /locus_tag="KR250_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBH0069161.1"
                     /translation="MSYMPTLEQAWEILRKYNKEEFHIRHAQIVSGVMRYYAKEYDPE
                     RVEFWEIVGLLHDLDFEMYPDEHCVKQRELMTELDLDKSIIDSTISHGYGLTGSDVKP
                     EQFMEKVLFAVDELTGLIGAAAIMRPSKSVSDLELKSVKKKFKDKSFAAGCSRDVIRS
                     GAEMLGWELDKLIQTTIEAMRSLIPEMAI"
     gene            complement(10348..11688)
                     /locus_tag="KR250_003271"
     CDS             complement(10348..11688)
                     /locus_tag="KR250_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBH0069162.1"
                     /translation="MIKSSLFWNIFIAISFMIEWFLCKKMLDYTSEKRISNKKINFCV
                     VGIIVFADIIYFFDVPPNIRVIISMIITIAFYKISYDVGFIKSVLISLIYWMLLIATD
                     ALSISLTVWLNSINDMSSMGTGDIYRIESIILGKILLIVSLLLYRALKIKLNIEINKR
                     TLFYIVIPIVANIASFFVIFEYIFNPGSGSLIHNFQFVMISVVLLISSITLVLIAKKL
                     QQYNTLVVENNMIKNTLKYENEYYEDIEKKYLKTRLLSHDMKNHILCINAMIKKGIDV
                     TSYLNNLQVEIQSNDLLFNTGNFILDAILSEKKEVCDKNDIELKIGINFSCCEAVELV
                     DICHIFSNILDNAIEACNKIDSGNRVICVKGDVMQNFYLIRVENTKMNKIITKNGDIL
                     TDKKGSLHGLGIKSVKNSVNKYGGNVVIEYDDKKFVVKIAIPLRNVKKAVNHVI"
BASE COUNT         4104 a         2046 c         1620 g         4279 t
ORIGIN      
        1 cagcatattt atttgatagt aattctattg caaatattta cagagtattt cctatatcat
       61 ttagctttat agttacagga ctatatttca gttataaaaa taataaaaat aataagatta
      121 actttgaggc agttttagag gacaaaaaaa cttaattttc ttattgagcc ttatgtttgg
      181 aatgtacttg tatctatgtt tgagtttgtc aatatactat actgttggaa atgtattgat
      241 tttaaaatta tatggaatca agtctgccat aatagttttg gtaggtaact atattgcaga
      301 aagatatagc tttagaatta ctcaaataaa aggttatgat attaaaaatc aaatagaata
      361 tgtagaaagt gtaaagacag agtatgagat taataaattg aatgatattg tatatacaga
      421 tttactgaca ggtttctaca atagaccttt tgtgaatcaa aaactatcag aatggacatc
      481 aaatcactac aaatttacct tatgttttac tgatttaaat agcttaaaac ctgtaaatga
      541 taaatttgga caccaatttg gtgataaata catatccact actgctcaaa taataaaaaa
      601 acttataaat gtagaaaatt ccttactatt tagatatggt ggagatgaat ttttattcct
      661 tttacagaat acaagttgtg tagaagcaga aaagataatg attaatataa attatgaatt
      721 atcaaaaata agtaataccg acgagtatcc ttatgctctt tccataagct atggattagt
      781 tgagtgggat ggaatatccg atattgaaac tttaatatca aaggcagatt ctttgatgta
      841 tgaaaataag cttaaaaata aatctacttt aaactagcta gttcagatat tctacaaaat
      901 atatattata agatagtact aagaatgtca aaatatatag attttgcaaa tattgaatac
      961 agtttactaa tgaatgcact ggatgtaagt gttagtaaac acctattaga tgaacacttt
     1021 actgttgttt gggctaatga ccgttattat gaaatgttcg gctataccta gctaaaaagt
     1081 taaaaatgac cactgtagcc gagggaatag agactagttt tcaaaaagat ttcttgcaag
     1141 aaatagactg tgatatgtta caaggatacg tgttctctag acctttacca ataaaagact
     1201 ttgaaaaact tatgtttcaa aactcctcca attaacaata taaaattgct tattataagt
     1261 gatattattg tatcaacaaa taaaggaatt gtctcaaaat tgattttttc taatcaaatt
     1321 tttattttga gacattctca tttttttgca taagaaaata cccaccaagc tacggttttt
     1381 cttaccgctg ttcggtgggt attagtagta ttatgaaatc tgaaaacttt aagtattact
     1441 acttccgcta gcgcaatcac aaaggtcgaa agcttcaaag cgaaaatagg ctttttactc
     1501 atcaaacgtc accgtacctg ccaacccgtc caccgttatc atttgtccgc tcttaatagc
     1561 tcgtgtggca atgtgtgtag ccataacggc tgggatgcca tattcacgtg cgacaatgct
     1621 tgaatggcta agaggaccac caatgtccgt tacaatcgca cttgcagatg aaaataatgg
     1681 tgtccatgct ggagttgtgg taactgcaac caaaacactt ccaggttgaa agctttcaaa
     1741 atctgctgga ctattcagta cacaagctgg agccgtcaca acaccagtac tggtaccaat
     1801 gcccgtcaac acaattttcc catctttctg cttctgttgt gcttgtgata tagttttttt
     1861 gtttttctct ggcaacttgg atggtgacac ataacccatg tattttttaa gttcagcttt
     1921 tcgaacaggt attatcccgt tcatatcaga tagagacttg tttttatcca actgctctat
     1981 aagtacctcc aattccgatt ttgtgagcca ataaatatca tctaaatgtg aaatagctcc
     2041 tcctcgtatg aatcgttctg agatttcctg aaacatacaa cgaatcagag gatgtcccat
     2101 tcccatcaaa taaatagcat tttcacgcat aggagaggtt tcctgtgccc aatggagcaa
     2161 cttaaaaaat agttttttac gcaatccacc tatgtgttgt aaaatctctt cttctgcatg
     2221 ttttctacgc ttttcaaacg tggtctggcg taaaaaagga ctttcccctt ttccttccac
     2281 aaatgttttt acggattcaa aagttggtgt aagtgtttcc tgcggtgtgg aatgcgcaaa
     2341 atcaaactca taagcggtac gaccaaactc tttaaagtac ttattgattc gatttttcca
     2401 ttctatccat acttcctgtg aaactccaac aggcacaact gaagacatga aatcttctgc
     2461 tatctttatt gcaggactgt tttgcagata aaggttgatg ctgttatttt gctttaccca
     2521 ttctgaaata ccccacaact ccttttccga ttgcagaaca atcgtatcaa acccaagcag
     2581 gaaaacggaa gtatccgtta taccagcacg tcgaactgct ccttgaaata attttgtaaa
     2641 taatgtttca ctgatagaag cagcaggcaa tgtaagctga atccttgtga aataaataca
     2701 tgcggcataa aatattgtct gaataccctc cataatttga tgggcgttca tagtatgcag
     2761 gggcttttct tcccattgtt taatagcatc ctcatatcct tttcgtgccg tttcccagcg
     2821 tgcaacgctg ttcataagtg tcctacgtaa agagcgtggg gaaagggatt tgatagcaat
     2881 caaaagtgga tttgatttag cagaaagata aacatatcca ttaataaccg tatatgcccc
     2941 gttttttggt aataattttt tcgcactttt accaaacata tctatccata aaagctctga
     3001 tgcacggttt acgatttcaa gaccaagcgt ggcgaacaaa ggtgtaacag gatttggcaa
     3061 atgttctgcc aaacttccct ttgtgtatac aacatcctgt tctggcaata cccattctgg
     3121 tggcagaaca gtaattggac gagcctgaac aatgtacaat ttatcttttt ccagtgccca
     3181 ctctacatcc ataggcattt gataatactt ctcaattttc tttccaagct gtatcagttg
     3241 cattacctga ttacgagtaa gagcatattt ttttctcaac ctttcaggaa ccatggtttc
     3301 ttctgttcct tctgaagtac gaaccgtcat aatctcttta tttgccactt catacgatat
     3361 aattcgttcg gagtccttat ccactacgat tgtatcgggt gtaactaagc tagaaaccac
     3421 cgcttcacca agcccccatg cagcgttaat aatcatttcg ctacgtctac cattgattgg
     3481 gtttaacgta aacataatac cagatgaatc ggaaaatgca agcttttgta ctacaacagc
     3541 aagtgcgaca atctcgtgct ttatattatt cttcacacga taagcaatag cacgagctgt
     3601 ccataaggaa gcccagcatc tctttacaga gtcaataact ttatcttcac cttgtatgtt
     3661 aagataagtt tcctgctgac ctgcaaaaga agcgtcaggt aaatcttcag cagtcgcaga
     3721 cgaacggaca gccaccgata tgttccctaa tccagcataa gccattttaa ttgcgtccga
     3781 cacttcctgt ggcatttctc cattatggaa aagctcccct attttcttag atacatcttc
     3841 aagttggctg gtattattgg aatcaatacc atctagcagt ttattaattc gagactgaat
     3901 atggttcgtc tcaacaaaaa tctggtatga agcagtcgta acatgaaaac cgtctggcac
     3961 agggatgcct gctgtcaata gttttgataa agacatccct tttccaccaa caatttcaat
     4021 ggttgcttgt ttatgttcca gtggcaagac ataataaatc ataatcattc acctccatca
     4081 ttcataattc cgtgcagaaa tatattcatt gtttcatcca acattttttc cgaactatcc
     4141 aatggtcgtg acgtgtagct gattgataac atggagttaa ttaacaaacg tgtcacaaga
     4201 gcggtatccg ttttgcgaaa gatgcctgtg gtcacaccat tatcaattac cgattttata
     4261 atatcttgat atgcataacg agcatcttta agtctctttc gatactcttc ttctaaataa
     4321 tcagattctg tatttaacca catcagtaca gttctatact gctctggaaa tctaagatga
     4381 ttaagcatga tttttctaag gcactgttgt ggtgaaggtt caccatcaat aatcctatga
     4441 acttgttgga tagttttttc tatttctttc tcaactgcat atacaacaat ctcgtctttc
     4501 gttttgaaaa aatcgtatag acttgacttt cccatgttgg ctaaaactgc aatttctctc
     4561 attgatgttt tttggaatcc attttcagcc ataagactca aagcaacagc aactattttt
     4621 tctcggcgtt ctgcttgttg ttcgggtgta agcacaattc cttttggcat agggcgatac
     4681 ctccttttag cgaccactgg tcgtttcttt tttatttatc atacagcgac caacggtcac
     4741 ttgtcaaggt taattttttt cattgtatat ggtactcgag ataaaaatgt tcattttaga
     4801 aattgctcta tttaagtaga aataggcttc atcattagaa aacaagacta taaatttgag
     4861 atatttatca tgttcaaatt tcatcaatat caaagtttaa gatattaata aaaaaagaat
     4921 tactaagaaa taacatataa agattcaaaa aaggagttga acttaaaatt caattacaaa
     4981 aaatcaaatt taagacaact cctttattct ttatctctac tattctttat ctctatttat
     5041 ttattacaac tccctaacat ttaccactac tgccttaggt ctagtcatag cctcgattga
     5101 atacttaaca ccttgagttc ccataccaga tgcctttaca cctaaaaatg ggaagtggtc
     5161 tggacctctt tcagtcttat tatttatttg aacagtacca acttcaagtt tatcagctat
     5221 ataaaatgcc ttatcaatat cacttgtaaa tactgatgat tgaagcccat actctgattg
     5281 attagcaatt tcaattgctt gatttatatc tttaactctt ataattggaa gtactggacc
     5341 aaatggttct tcccaagcaa ttttcatatc aacatttaca ttatctaaca atgttggata
     5401 gaataaatta ttttttctaa cattgcctgt tattaacttt gctcctttat gtaatgcatc
     5461 atcaaccaac ccttgaacaa aatcagttgc tttattatct ataagaggag tgataactac
     5521 ttcatccatt ggatttccaa ttgtcaactt ctctaccaat ggctttattt ttccgactaa
     5581 tttatccgcc acactctctt gtactaaaat tctctttact gccgtacatc tttgtcctga
     5641 atatgaatac gctccagata ctatattctt agctgcaaaa tctaagtctg catcttctaa
     5701 tactatagcc gcatcttttc caccaagctc caataataat ggtaccattc cagatatttt
     5761 agatatatgc tgaccaactt cagtactacc agtaaagtta ataaaattaa ttcctttatg
     5821 cgttacaaca taatctccta tctcactacc cctacctgta atagtattaa gtactccatc
     5881 tggtatccca gcttttctca taatttctac cacatgaagt gcgctaatag ccccttgtgt
     5941 agcaggtttt aatacaactg catttccacc aataagtgct ggagcaatct ttgacattga
     6001 taaatttaca ggataattaa aaggagatat agctaaaaca gtacctaatg gaacccttga
     6061 aacataagac atcttatttc ttgttccacc agggaaatta tctccactaa tggcttcacc
     6121 ctccatattt tttcccacat cagctgtgta tcttaaaaaa tctgctgttc tctcaacttc
     6181 tgcccttgca gattttatgt ctttggctat ttcaagtacc agtatattag ctatctcatc
     6241 tatattttct aacaataaat cagcagcctt atgaaatata ttggctctct tatatacagg
     6301 catttcagcc catactttta tactctcttt tgtgtttttt ataacctcat ctacctcatg
     6361 ttttgtcata gattgtactt tcccaacaag agaattatct attggtgagt aaatttctat
     6421 tggctttctc gaattagatt ctacccattt tccatttatt aaatttttat atacattttc
     6481 ttttgtttta agttcattaa acatattttt attcctctct ctgatataac tttgtgtatt
     6541 taaattatag ccatttattc gttatgtaaa catttctttt tttcataaac gtttacctat
     6601 aaaaaaattt taagttaacg tttttctaca ttcttctatt ttaataacat actcgctcca
     6661 aatctatata ggtcacactt taatatcaat atactaatat accaatatac caatatacca
     6721 atataccaat ataccaatat aaatattata aacataaatc acttgacaaa tttataagta
     6781 gacaatatta tatatctatg acttaaaata tttttaaagt catagatatc atatatagat
     6841 agaaaggatt gataaaaatg gctaaaagtt ttacagaaat agaaaaagac aatataagaa
     6901 aaagactaat ttcagaatgt gaaatcaact ggtctaaata tggatacaaa aaaactaata
     6961 ttgatgaact ttgttcaaaa tcaggaattg caaagggttc attctacaca ttctttgatt
     7021 caaaagaaaa gttatttttt gaagtattaa atggaatcca aaacagactt atatcatcag
     7081 tagatgaaat tttaagtaac atgcctccaa aagaaggctt tgcaaaagcc ttaaagatgt
     7141 tgtatcgatt atatgataaa aacccattct tatattcacc aaataatact gattacatca
     7201 ctttattgaa taagatacct aaagagatgc tttcacaact tgaatacagc aatgtacaaa
     7261 gtttttatag cttaattgaa agacataatc tcaagttaaa aatagagaaa gaaaaagcct
     7321 ttggtgtttg caatgcccta ctgtcaagcc tattgctaaa agaagatatt ggatatgact
     7381 acattgaagt atttgatttt atggtagaca atcttgtaga acacatactt gagtaataat
     7441 ttaaaataaa ttttctaaac atttctttat aactaggagg agtaatatga ttgaattaat
     7501 acaaagacta aatgatatta tttacaaaat aaatgataaa ctaaatatcc acacaaaact
     7561 tgtagaccct agcagtgagc tcatgccact ttactctatg ttatctataa tcgtttctat
     7621 atttctaata atattaggta ttaaagtaag aaaaaaattc cttataataa ttgctattat
     7681 atcaattatt ataaatattt taataatctt agataggtag gtacatatat ggtaaaatcc
     7741 tcgaacactt ttaataaaaa cttcaacttg atgatattag gtcaaataat ctcattgttt
     7801 ggtgcatcaa tacttaaatt tgctttatct ttatacatac ttgatataac tggtaaagca
     7861 gaaatatttg ctactatact agcagtatct tcaatcccaa ttatagtatt ctcccctatt
     7921 ggtggagcta ttgcagatat attcaacaga agaaacttaa tggttatttt tgatttttca
     7981 agtagtctta cagtactaat cttagcagta ttcttattca acaataatgg ttcaatatta
     8041 cttgtaggaa ttattatgac catattatca gtcataagca caatgtatca accaactgtt
     8101 cagtcaagta cacccctttt agttgataat gagcatttaa tgaatgcaaa tggtattgta
     8161 gctggagttg catctcttac aaatattgca ggacctgtat taggaggtgt cttgtatggt
     8221 gtgattggaa ttaatgcaat tatagttata agttgtgtaa gtttctttct atctgcaatt
     8281 atggagatat ttatacagat tccttttatc aaacaagagc aaaacagcaa cattataaaa
     8341 actatattta ctgatataaa ggatggtatt gttcatatat gtaaagaaaa tagatttata
     8401 ctaaaaatac tttttttagc tatagcaata aacttatttt taagctctat gttaatagtt
     8461 ggtatcccct atatagtaaa aatcactatg ggagctagta acttcttata tggtctagca
     8521 gaaggagcaa tttcttttag ttcactggta gctgctgtta gtattggtat tttctcaaaa
     8581 catctaaaaa tcactaattt gcatatttcc tttattatat gtgcagttat gttcatacca
     8641 atggcggttt cagtatatcc atttatatta aacacaggat ttccagttcc atttttaatc
     8701 tttatagtat catctatgct aatatttttt acaacgactc ttatctcaat atatgttata
     8761 acagtcacac aattaaaaac tccaaatgaa ctcttaggaa aagtaatgtc aattcttttt
     8821 gcaggaacag caattgcaat gccccttggt caggttatgt atggtaaact ttttgattta
     8881 ttatcagata gagtgtacat cattatacta ggagttggat ttattacatt acttatagca
     8941 tttgtatcaa agataacatt gaggaacgaa aatctttaga tacaaagaat ttagatataa
     9001 agatttaatt ttcactaatt tctgaattta aatgcaaaat tgtgtatcag tatgttgacg
     9061 ttttagaaat tgattgatat aatcaatgga aaataaactt tataaaaatt caaaatattc
     9121 agctagagga gtgataaaaa tgagttacat gccaacttta gaacaagcat gggaaatatt
     9181 aagaaaatac aataaggaag aatttcatat aagacatgct caaattgtat ctggagttat
     9241 gagatactat gcaaaggagt acgaccctga aagagtagaa ttttgggaaa tagttggact
     9301 tttacatgat ttagattttg aaatgtatcc tgatgaacat tgtgttaaac aaagagaatt
     9361 aatgacagag cttgatttag ataaatcaat aatcgattct acaataagtc atggatatgg
     9421 attaactggt tcagatgtta aaccagagca atttatggag aaagtattat ttgctgtgga
     9481 tgaacttact ggtttaattg gtgctgctgc aataatgcgt ccttcaaaga gcgtttctga
     9541 tttagaatta aaatctgtta agaagaaatt taaagataaa tcatttgctg ctggctgttc
     9601 tcgagacgtt ataagaagtg gtgcagaaat gcttggatgg gaacttgata agcttatcca
     9661 aacaactata gaagctatga gaagcttgat tccagagatg gcaatatagc tcctccatac
     9721 atataccata gaagttacat ctttaaagag catttctctt tttgatgcaa cttcttttta
     9781 tattttgtag aataaaaagt ttaatatttt aaactcacta tataatctat aataaatact
     9841 ataaataata taatgtataa tagtcataaa ctttctaacc ttaaaagttt ctaataatca
     9901 ataaacaatt ctaatataca aatgtcatta tatgatttaa ttatatatct tcaatttata
     9961 tattaaaata aatatatata tattaaattc ttatacacca aatttttaca ttaaacttct
    10021 atgcaccaca tttatacata ttaaattctt atatattaaa tttctatgca tcacttttat
    10081 acatattaaa ttcctatata ttaaactttt atgcatcact tttatacata ttaaattcct
    10141 atatattaaa cttttatgca tcacttttat acatattaaa ttcttatata ttaaacttct
    10201 atacatcact tttatacatt aattcttata tattaaactt ctatacatca cttttataca
    10261 ttaattctta tatattaaac ttctatgcat cacttttata cattaattct tatatattaa
    10321 atttctacac atcaaattta tatacattta aatcacatga ttaactgctt tcttcacatt
    10381 tctaagtgga atagcaattt taactacaaa ttttttatca tcatattcaa ttacaacatt
    10441 accaccatac ttatttacag agttttttac actttttatt ccaagtccat gaagagaacc
    10501 ttttttgtct gtcaaaatat ctccattttt agtaataatc ttattcatct tagtattttc
    10561 aactcttatt aaataaaaat tttgcataac atcaccttta acacaaataa ctctatttcc
    10621 actatcaatt ttattacatg cctcaattgc attgtctaaa atatttgaaa atatatgaca
    10681 tatatccact aattcaacag cttcacaaca actaaaattt attcctattt ttagttctat
    10741 atcattctta tcacaaacct ctttcttttc acttaatata gcatctaata taaaattacc
    10801 agtattaaat aacaagtcat tactctgaat ttctacctgc aaattattta aataagaagt
    10861 aacatcaata ccttttttaa tcatagcatt tatacacaag atgtgatttt tcatatcatg
    10921 acttagtaat ctagttttta aatatttctt ttctatatct tcataatact cattttcata
    10981 cttcaaagta tttttaatca tattattttc tacaactaaa gtattatatt gctgtaattt
    11041 tttagcaatt aaaactaaag tgatacttga tataagcaat acaacagaaa tcataacgaa
    11101 ttggaaatta tgtattaagc taccagaccc tggattaaat atgtattcaa aaattacaaa
    11161 aaaagatgct atattagcga ctataggaat aactatataa aataatgtcc ttttatttat
    11221 ttctatatta agttttatct ttaaggctct atataaaagc aaagacacaa tcaaaagtat
    11281 ctttcccaat attatagact caattctgta tatatctccc gtccccatac tactcatgtc
    11341 atttatagaa ttaagccaaa ctgttaaaga tatactcaaa gcatctgtgg cgattagtaa
    11401 catccaataa attaaggata ttaatacaga ctttataaac cccacatcat atgagatttt
    11461 ataaaatgca atagtaatta tcatagatat aattactctt atatttggag gtacatcaaa
    11521 aaaatatatt atgtcagcaa aaacaataat acctaccaca caaaagttta tttttttgtt
    11581 agaaatcctt ttctcacttg tataatctag cattttctta caaagaaacc attctatcat
    11641 aaaggatatc gcaatgaaaa tattccaaaa taagcttgac tttatcataa tactttctcc
    11701 taacgtatta ctaaattatt tttttatatt ttatgtataa tttaattata tacgactcta
    11761 cacgtgtagt aaatagattg tcaaaattat tccaaataag tccatatttc ttttcttttt
    11821 taattatatc actaaatttg ctaaattgaa ttaaactttt tattgctttt tttaataata
    11881 actatataaa tttatctata actaggtata aaatatatta tttataaagt ttttacatta
    11941 tttccataat aaaatcttat aaatattaca ataaaatatt tttttccatg atataccttg
    12001 ataaaatctc tgcttcttta agcagtaact ttgagttgaa tcattatag
//
LOCUS       DAEPIN010000095        11466 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.095, whole genome shotgun sequence.
ACCESSION   DAEPIN010000095 DAEPIN010000000
VERSION     DAEPIN010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11466
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.095"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            249..2159
                     /locus_tag="KR250_003272"
     CDS             249..2159
                     /locus_tag="KR250_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HBH0069163.1"
                     /translation="MNSNTFERLQLNEVKELIKIHCVSSLGKNLIDKLTPSGNIGVVR
                     RKLAENKEARKIIENSNHIPLEGLFNAGSTIDKIEKGMIIEPVELVNIEDFLRGCRKM
                     KAFMLEKEFYSPTLSSYALNITECKSIEDEINYCIKSNKVDSNASKELKKIRRNIEIT
                     EGKIKDRLNKFITSTVNKKYIQEFIISKRNDRYVIPIKSSYKNEVNGTILDTSSKGNT
                     VFIEPISVSNLSTELTMLKADETIEEYKILSYLTELIFEKISQIKLNIEILSEYDMVF
                     AKAKYSQKIKGITPKINNNGYIKIIKGKHPLLTGDAVPLDFEIGKNYRSLIITGPNAG
                     GKTVTLKTVGLLTLMVQCGFDISAKEGSEFSVFEKVFVDIGDNQSIENALSTFSSHIK
                     NIAEIMSLSNNSTLVLFDEIGSGTEPNEGAGLAISLLEEFYKMGCITIASTHYGEIKK
                     FATLHPEFENAGMMFDKETLEPLYKLTIGKSEDSNALFISKKMGIKNRVLDRAKEYVI
                     DRNYNLDLVKRSKLQSNIEDKNSSEVILNYTDYNVGDKVKLLDEDDFGIVYKPMDKFN
                     NVEVLFNDNFISVNIKRLQLSIKASELYPEGYDLDVLFTSFKERKLEKDIQRGSKKAL
                     KKIQKEIRENRK"
     gene            complement(2222..2845)
                     /gene="ytaF"
                     /locus_tag="KR250_003273"
     CDS             complement(2222..2845)
                     /gene="ytaF"
                     /locus_tag="KR250_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation membrane protein YtaF"
                     /protein_id="HBH0069164.1"
                     /translation="MIESILLVTSLCIDAFVTSFGYGVNKVKVPLYSNIIISVVCSAI
                     LAISLFAGTIVRDFLPQNITMLICFFILFLLGSARLIESFFKSYFKKKSIHSKNYNFN
                     MLDLKLNFNVSIEPDVLDTSKSKVLKSAEAFTLALALSLDGLAIGFGSGLVGVNYIQV
                     ILFSLISNIIAVLLGCLLGNMFVKNVNLNLSWLSGLILLVIGFMKIC"
     gene            3033..3677
                     /locus_tag="KR250_003274"
     CDS             3033..3677
                     /locus_tag="KR250_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH0069165.1"
                     /translation="MEQHSKVGNFGDLLKVLLDEKELSMGELSKKSGIDKSTISRIAN
                     NKQKPNINHLEKMAIHLNINLEELLKASGYEFRNSNNQIFNADSDFSNFDDILGFANL
                     INDKNFNGNIEKELSKCKLYVQTDEGKKLLFDNFNKKIDSIEKQGQFTDRLRQMYADF
                     CTDGLTIKKYLLIGSMLLYFVISTDVIPDFVFPIGFMDDLVALNIVTKLLKNDK"
     gene            3891..4949
                     /locus_tag="KR250_003275"
     CDS             3891..4949
                     /locus_tag="KR250_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HBH0069166.1"
                     /translation="MNCKWISKIDERKKCHREADSSGYCIFHKENKSDEEIQLMMDTL
                     HKEEISEFNGFVFENEFNAEEILTYNYKILDFSESIFKQKANFKKYIFKKNIIFNYTE
                     FRDKVLFNGCVFLENCDFNRTIFSKHYINDRIFEKVKFKGPDLVVNKVENFPRMDGII
                     FSMCTKFVLKNVEYGKSEYEHGKINYRIARNQATKIGEYEMIGFYYYKERIYSSKIMK
                     RSNYPTFSDYLVEKFFDQIARYTTGYGEKPWNILLVIIAIISVFALLYLFVGIESSNS
                     TLVALDINNIGDYSLSEIFKMYMDLWYFSMATFSTVGYGDMVATSLIGKALAGIEVFF
                     GVTIGAIWASVIIKRMIR"
     gene            5053..6237
                     /locus_tag="KR250_003276"
     CDS             5053..6237
                     /locus_tag="KR250_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBH0069167.1"
                     /translation="MKNALKKIISLAMILVFIIPNYSYADDKSVDKYSKTSILIDQET
                     GRVLYSKEPDMKVPLASLSKMMTFLLAIEAIENKEVKETDKVKIDKSIASVKGSSYKL
                     KDGEEVPLIELMRGLMIVSGNDAAIAIAKHVCKTKEAFVDRMNKKAKEIGMSNTHFLN
                     PNGLPIYDLSNPKKPAKENISTAKDIAILGKYMFDHYEKQVTAITDMETYTNSDRSFE
                     KSNTNALLRIIPEVDGIKTGYTGNAGYCLSFSMVVNKNDKNEKKRRVIGVVLGTNHKN
                     KRTSASLALLKYGKENFNMKKVIDKDSFIGKKYIKGTKELEVTLKAKDELYTILKDDE
                     SIKSEVKIKNIEYPVKKGDTMGIIKYYTNSGDLLGSVDIVSNNSIDNVSLKTKLKMKF
                     AN"
     gene            6444..7157
                     /gene="rpsB"
                     /locus_tag="KR250_003277"
     CDS             6444..7157
                     /gene="rpsB"
                     /locus_tag="KR250_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HBH0069168.1"
                     /translation="MSVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIYIIDLQ
                     KTVKKVEEAYKFTKEVAETGKPILFVGTKKQAQDAIKDEAERCGMYFVNERWLGGMLT
                     NHKTIKTRINKLRELEKMEEEGVFNVLPKKEVIKLRAEKEKLEKYLGGIKDMPELPGA
                     MFVVDPRKENIAIQEAHRLGIPVVGIVDTNCDPEQLDFAIPGNDDAIRAVKLITGAMA
                     TAVIEGRQGAEEEVAEDQE"
     gene            7236..8147
                     /locus_tag="KR250_003278"
     CDS             7236..8147
                     /locus_tag="KR250_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HBH0069169.1"
                     /translation="MANITAQMVKELRESTGAGMMDCKKALQEAEGNMEKAVDLLREK
                     GLSKAAKKAGRVAAEGLVAIEMNDDNTVASMVEVNSETDFVAKNEDFKVFVKDAACMA
                     LATDKEDIASLLGETHKEGITLQEVLNNRVAKIGEKLDFRRFAKVVTNGQVAGYIHGG
                     GKIGVLVEMETEARDAKVLELGKDVAMQVAAMNPKYVSRDEVDAEYIAHETEVLTQQA
                     LNEGKPANIVEKMVKGRLEKELKEVCLLEQTFVKNPDITVKQLVADVAKAVGSDIKVV
                     KVVRFEVGEGIQKREENFAEEVAKQLK"
     gene            8232..8936
                     /locus_tag="KR250_003279"
     CDS             8232..8936
                     /locus_tag="KR250_003279"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HBH0069170.1"
                     /translation="MDKPMYKRVLLKLSGEALAGERGFGINNDVVNDIAIAIKKIQEI
                     GVEVAVVVGGGNFWRGRTSEGMDRTTADYIGMLATVMNAMALQDALENIDVATRVQTA
                     IDMRQIAEPYIRRRAVRHLEKERVVIFGAGTGNPYFTTDTTAALRAAEMEAEVILLAK
                     NVDAVYDKDPKVHADAKKFTELSYMEVIQKELKVMDSTATSLCMDNKIPIKVFELTTE
                     NIIRAVKGENIGTTVK"
     gene            8962..9519
                     /gene="frr"
                     /locus_tag="KR250_003280"
     CDS             8962..9519
                     /gene="frr"
                     /locus_tag="KR250_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HBH0069171.1"
                     /translation="MKLEIHKQLEEKMNGTIDALKFEFGTIRAGRANAQMLDKIRVDY
                     YGTPTPINQIGAISVPEPRILMISPWDKSAMHEIEKAIANSDLGLNPSNDGEVIRLSV
                     PALTEERRKELAKKASKAAEEFKVRIRNERRDANEKIKKMEKGGELTEDELKKAQDEV
                     QKMTDKFIKEIDTLLSKKEKDIMEV"
     gene            9522..9725
                     /locus_tag="KR250_003281"
     CDS             9522..9725
                     /locus_tag="KR250_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069172.1"
                     /translation="MKKYYNLLGLHVDEVKQYFDEQNINYTVKSIEGKKDKEKLVVPR
                     VIKISEIGDSVELIITYFSDSLV"
     gene            9754..10488
                     /locus_tag="KR250_003282"
     CDS             9754..10488
                     /locus_tag="KR250_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HBH0069173.1"
                     /translation="MNNNIIYDIDLNNIPTHIAIIMDGNGRWAKARFLPRTAGHKAGV
                     ETIRDIVKECSKLGVKHLTLYAFSTENWKRPKLEVDTLMNLLSTYLRNEIAELHQNNV
                     KVTAIGDISALPKTCIRELNSAKELTKNNTGVNLNLALNYGSRADIKNALIDIVKNCE
                     SGKIDINNIDEDIIKNYLSTKSIPDPDLVIRTSGEQRLSNFLLWEVAYSEFYFTDIHW
                     PDFKKEELQKAIYVYQRRDRRFGGLK"
     gene            10501..11280
                     /locus_tag="KR250_003283"
     CDS             10501..11280
                     /locus_tag="KR250_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HBH0069174.1"
                     /translation="MLTRIIASLALVPLFLFVVYGGIPLYIAETAIVYIALHEFYKAF
                     KIKDVHPIFIIGYLFSIYLAVKNIFNLPLEYTYAVIFILFLASIIYMLMGKNNVIDVS
                     ITFLGVFYIGVFLDFIIITINGFEKGSIYVWLIFVISFMTDIFAYFSGYLLGKHKLIP
                     KVSPKKTIEGAIGGIIGSTLCCILFGYLFGIDLLQLAIIGSIGSVIAQLGDLFASSIK
                     RYVGIKDYGKIIPGHGGILDRFDSVILVAPFVYSAIKFFIR"
BASE COUNT         4819 a         1162 c         2021 g         3464 t
ORIGIN      
        1 taaatatata atttatggaa taatattaga agcactgata taagtgttta aataagaata
       61 gaaaaggaga agtgttttat gaaaaaaaat actaacatta atgagtccgg agttaaactt
      121 cttaataaat aaggagtttg atatataata actccttatt tattaagttg ttcgcaaaat
      181 aactattatc aaagtaaatg atagggtgat ttaaaagacg ataacttata aataaaggag
      241 aaaaaataat gaatagtaat acatttgaaa gattacaact aaatgaagtt aaagaattaa
      301 taaaaataca ttgtgtaagt tcactaggaa aaaatttaat agataaatta actcctagtg
      361 ggaatattgg agtagtaaga aggaaattag cagaaaataa agaagctaga aagataattg
      421 aaaatagtaa tcacatacca cttgaaggat tgtttaatgc aggttctaca atagataaaa
      481 ttgaaaaagg aatgataata gaacctgttg aacttgtaaa tattgaagat tttttaagag
      541 gttgtaggaa gatgaaagct tttatgttag aaaaagaatt ttattcacct acattaagtt
      601 cttatgcctt aaatataaca gaatgtaaaa gtatagaaga tgagataaat tactgtataa
      661 aatctaataa agttgattct aatgctagta aagaattaaa aaagataaga cgaaatatag
      721 aaattacaga aggaaagata aaagatagat taaataaatt tataacatca actgtaaata
      781 aaaagtatat acaagagttt ataataagta aaagaaatga tagatatgta ataccaatta
      841 aatcttctta taaaaatgaa gttaatggta ctatattaga tacatcatca aaaggaaata
      901 cggtatttat tgaacctatt agtgtttcaa atttaagtac agagttaact atgttaaaag
      961 ctgatgaaac tattgaagaa tacaaaatat tatcttattt aacagaactt atatttgaga
     1021 agataagtca gataaaatta aatatagaga tactttcaga atacgatatg gtatttgcaa
     1081 aagctaagta tagtcaaaaa ataaaaggaa ttactccaaa aataaataat aatggctata
     1141 taaaaataat taaaggtaaa catccacttc taacaggaga tgcagtacct ttagattttg
     1201 aaataggcaa aaattataga agtcttataa taacaggtcc taatgcagga ggaaaaactg
     1261 taacccttaa gactgttggt ttactaactt taatggttca atgtggattt gatatttctg
     1321 caaaagaagg tagtgaattt agtgtatttg aaaaagtttt tgtagatata ggggataatc
     1381 aaagtattga aaatgcctta agtacttttt catcacatat aaaaaatatt gcagagatta
     1441 tgagtttatc aaataattct actcttgttc tgtttgatga aataggaagt ggaactgaac
     1501 ctaatgaagg agctggttta gcaatatcat tattagaaga gttttacaaa atgggatgta
     1561 taactatagc atcaactcat tatggagaga taaaaaagtt tgctacattg catccagaat
     1621 ttgaaaatgc tggtatgatg tttgataaag aaacgttaga accattgtat aagttaacta
     1681 ttggaaaatc tgaagatagt aatgctcttt ttatttctaa aaaaatggga ataaaaaata
     1741 gagttttaga tagagctaaa gaatatgtaa ttgatagaaa ctacaattta gatttagtta
     1801 aaagaagtaa gctacaatct aatattgaag ataaaaatag ttctgaagtc attttaaact
     1861 atacagatta taatgtaggg gataaggtaa aattactaga tgaagatgat tttggaattg
     1921 tatataagcc tatggacaag tttaataatg tagaagtatt gtttaatgat aattttattt
     1981 cagtaaatat aaaaagatta caacttagca taaaagcatc agaattgtat ccagaagggt
     2041 atgatttaga tgtattattt actagtttta aagaaagaaa attagaaaaa gatattcaaa
     2101 gaggttcaaa aaaagctctt aaaaagattc agaaagaaat tagagagaat agaaaataaa
     2161 tataaaaagc acaaaagaaa gcaatctaaa gagaaaagtt tatctttaga ttgctttctt
     2221 attaacatat cttcataaat ccaatgacta acaagattaa accacttaac caagataagt
     2281 ttaaatttac gtttttaaca aacatatttc ctagtaaaca accaagtaat actgcaatta
     2341 tatttgatat taaagaaaat agaataactt gtatataatt cacacctaca aggccactac
     2401 caaaaccgat agctaatcca tctaaactta aagcaagtgc aagtgtaaat gcttcagcag
     2461 atttaagcac tttagattta cttgtatcta agacatcagg ttcaatactt acattaaaat
     2521 ttaatttaag gtctaacata ttaaaattat aattctttga gtgtatagat tttttcttaa
     2581 aataagattt aaaaaaactt tcaattagtc tagcacttcc taataaaaat aaaataaaga
     2641 aacatattaa cattgttata ttttgaggta aaaagtctct aactattgta cctgcaaaaa
     2701 gcgatattgc taaaatagct gaacaaacta cgctgataat tatatttgaa tataagggaa
     2761 ctttaacttt atttacgcca tatccaaagc ttgtcacaaa ggcatctata caaagagaag
     2821 taactaataa aattgattca atcacagtag attaacatcc tttctcttaa attttgtact
     2881 tatattagtt atagtattgt aaaaatggat aaattgttac agataatcca ataaattgtt
     2941 gataaaattc caacatcata caacacctaa ataacaaaag aatcatatca tataataata
     3001 taaaaccaat cataataaag caggtgaaaa taatggaaca acatagtaag gttgggaatt
     3061 tcggtgattt gttgaaagtt ttattagatg aaaaagaatt atcaatggga gaactaagta
     3121 aaaaaagtgg aattgataaa tctactatat ctcgtatagc taataataaa caaaagccaa
     3181 atataaatca tttagaaaag atggcaatac atttaaatat taacctagaa gaactgttga
     3241 aagcttcagg atatgaattt agaaacagca ataatcaaat atttaatgca gatagtgatt
     3301 ttagtaactt tgatgatatt cttggatttg caaatttaat caatgataag aactttaatg
     3361 gaaatattga aaaagagtta agtaagtgta agttatatgt ccaaacagat gaaggtaaaa
     3421 aattactttt tgataatttt aataaaaaaa tagattctat tgaaaaacaa ggtcaattta
     3481 cagatagatt aagacaaatg tatgctgatt tttgtacaga tggacttaca ataaagaaat
     3541 atttattgat tggaagtatg ctactttact ttgttatatc aacagatgtg attccagact
     3601 tcgtgttccc aataggattt atggatgatt tagtagcatt aaatatagtt acaaaattac
     3661 ttaaaaatga taagtaagtt ttagaatcat atttaatata taaataaatc aaagtatata
     3721 aaatttatta ttttttatat gaaacaaatt aaaagcacaa tgaagtagat taaaaattta
     3781 aaactaagaa tctaatatat attttgtaac tatttaagta tataaaataa aataatatcc
     3841 aaactaatac aaaatagtaa taattgtttt gtactggagg atatatattt atgaattgta
     3901 aatggatatc taaaatagat gagagaaaaa aatgtcatag agaagcagat tcttctggat
     3961 attgtatctt tcataaagaa aataagtctg atgaagagat acaattgatg atggatactc
     4021 ttcataaaga agagataagt gaatttaatg gatttgtttt tgaaaatgaa tttaatgcgg
     4081 aagaaatatt aacttacaat tataaaatac tggatttttc tgaatccata tttaaacaaa
     4141 aagcaaattt taaaaaatat atttttaaaa agaatattat ttttaactat acagaattta
     4201 gagataaagt tttatttaat ggttgtgtat tcttagaaaa ctgtgatttt aatagaacca
     4261 tatttagtaa acactatata aatgaccgta tatttgaaaa agtgaaattt aaaggacctg
     4321 atttggtagt taataaagta gaaaattttc cacgaatgga tggaataatt tttagcatgt
     4381 gtacaaaatt tgtgttaaag aatgttgaat atggaaaaag tgagtatgag catggcaaga
     4441 taaattatag aatagctaga aatcaagcaa caaaaattgg agaatatgag atgataggat
     4501 tttactatta taaggaaaga atttatagta gtaaaataat gaaacgttct aattatccta
     4561 ccttcagtga ttatttagtt gaaaaatttt ttgaccaaat agcacgttac acaacaggtt
     4621 atggagaaaa gccatggaat atattattag ttattatagc aataataagt gtttttgctt
     4681 tattatactt atttgtaggc atagaaagtt caaatagcac acttgtagct ttagatataa
     4741 ataatatagg tgattactca ctaagtgaga ttttcaaaat gtatatggat ttatggtatt
     4801 ttagcatggc tacatttagt acagtaggat atggagatat ggttgctaca agtttgattg
     4861 ggaaagcatt ggcaggtata gaagtatttt ttggagttac aataggagca atttgggcat
     4921 ctgtgattat taaaagaatg ataaggtaat atttgtgtaa atatggatag gtattaatta
     4981 ctagatataa attggactaa atagggaaaa tttaaatata aatatactta ataatccaag
     5041 aaggtgaaaa atttgaaaaa tgcactaaaa aaaattatat cattagccat gattctagtt
     5101 tttataatac caaattattc atatgcagat gataaatctg tagacaagta ttcaaaaaca
     5161 tctatactta tagaccaaga aacaggtaga gttttatata gtaaagaacc tgatatgaag
     5221 gtaccattag ctagtttaag taagatgatg acttttttac ttgcaataga agctattgaa
     5281 aataaagaag taaaagaaac tgataaggta aaaatagaca aatctattgc atcagtaaaa
     5341 gggtcaagtt ataaacttaa agatggtgaa gaagtgccac ttatagagct tatgagaggt
     5401 cttatgattg tatcaggaaa tgatgcggca attgctatag caaaacatgt ttgtaaaacg
     5461 aaagaagcat ttgtagatag aatgaataaa aaagcaaaag aaataggaat gagtaataca
     5521 cattttctaa atccaaatgg tcttccaata tacgatttaa gcaaccccaa aaaaccagca
     5581 aaagaaaata tctctactgc aaaagatata gctattcttg gaaaatacat gtttgaccat
     5641 tatgaaaaac aagtaacagc tataactgat atggagacat atacaaattc agatagaagt
     5701 tttgaaaaaa gcaatacaaa tgcattatta agaattatac cagaagtaga tggtataaaa
     5761 actggatata ctggcaatgc tggatattgc ttatcatttt ctatggttgt aaataaaaat
     5821 gataaaaatg agaaaaaacg tagagtaata ggggttgtat taggtactaa ccataaaaat
     5881 aaaagaacct ctgcttcatt agctctactg aaatatggaa aagaaaattt caatatgaaa
     5941 aaagtaattg ataaagatag ctttattggt aagaaatata taaaaggaac gaaagagtta
     6001 gaggttacat taaaagcaaa agatgaactt tatacaatat taaaagatga tgaaagtata
     6061 aagtcagaag ttaagattaa aaatatagag taccctgtta aaaaaggtga tactatgggt
     6121 attataaaat attatactaa ttcaggtgac ttacttggaa gtgtagatat agtaagtaat
     6181 aatagtatag ataacgtatc attaaaaaca aaattaaaaa tgaaatttgc aaattaaatt
     6241 caaaaaatgg tataaaatac acaaaaaaat ataaaaatag aaaatatgga aaaaatattg
     6301 ttatttcttc acctctgtga tatactacta gaggtgaaaa aataaaataa atcacacaca
     6361 gcattgggat ccttaaaagg tgcctattac atggtttttt agggagaaga tcaagctgga
     6421 ggtataaaac caggaggtaa gatatgtcag taatatcaat gaaacaatta ttagaagcag
     6481 gtgttcactt tggtcaccaa acaagaagat ggaaccctaa aatggctaag tatatattca
     6541 cagagagaaa tggaatatat ataatcgact tacaaaaaac agttaaaaaa gttgaagaag
     6601 cttacaagtt cactaaagaa gtagctgaaa caggaaagcc aatcttattc gtaggaacta
     6661 aaaaacaagc tcaagatgcg ataaaagatg aagctgaaag atgtggaatg tactttgtta
     6721 atgaaagatg gttaggtgga atgttaacaa accacaaaac tataaaaaca agaataaata
     6781 aattaagaga attagaaaaa atggaagaag aaggcgtatt taatgttctt cctaaaaaag
     6841 aagttataaa attaagagct gaaaaagaaa aactagaaaa atacttaggt ggaataaaag
     6901 acatgcctga attaccaggt gcaatgttcg tagttgaccc aagaaaagaa aacatagcta
     6961 ttcaagaagc tcatagatta ggtataccag tagttggtat agttgatact aactgtgacc
     7021 cagaacaatt agactttgct ataccaggaa atgatgacgc tataagagcc gtaaaattaa
     7081 taactggtgc tatggcaaca gctgtaattg aaggtagaca aggtgctgag gaagaagtag
     7141 ctgaagacca agaataataa tttgacaatt aaatggtaag ggtcgccctt accatttata
     7201 atatggaata gaataagtag gaggaaactt aaaaaatggc taatataact gctcaaatgg
     7261 taaaagagtt aagagaaagt acaggcgcag gaatgatgga ctgcaagaaa gctttacaag
     7321 aagctgaagg aaacatggaa aaagcagtag atttattaag agaaaaagga ttatcaaaag
     7381 ctgctaaaaa agctggtaga gttgctgctg aaggtctagt tgcaatagaa atgaatgatg
     7441 ataatacagt tgcttctatg gtagaagtta actcagagac agatttcgtt gctaaaaacg
     7501 aagacttcaa agtgttcgtt aaagatgctg cttgtatggc attagctact gataaagaag
     7561 atatagcttc tttattaggt gaaactcata aagaaggaat aactttacaa gaagttttaa
     7621 ataatagagt tgcaaaaata ggtgagaaat tagactttag aagatttgct aaagttgtaa
     7681 ctaatggaca agttgctgga tacattcacg gtggtggaaa aataggtgtt cttgttgaaa
     7741 tggaaacaga agctagagat gctaaagttt tagaattagg taaagatgta gctatgcaag
     7801 ttgctgctat gaatcctaag tatgtttcaa gagatgaagt agatgctgaa tacatagcac
     7861 atgaaacaga agtattaact caacaagctt taaatgaagg aaaaccagct aatatagttg
     7921 aaaaaatggt taaaggaaga ttagaaaaag aattaaaaga ggtttgttta ttagagcaaa
     7981 ctttcgttaa aaatccagat ataactgtta aacaattagt tgctgatgtt gctaaggcag
     8041 taggttctga tataaaagtt gttaaagttg tgagattcga agttggtgaa ggtatacaaa
     8101 agagagaaga aaactttgca gaagaagttg ctaagcaact taagtaataa ccaaagagag
     8161 aacacgcagg tgttctcttt tttaaaaaat agaaaaaatt ggatatgaag gattgaaata
     8221 ggaggtttct gatggataaa ccaatgtaca aaagggtatt gttaaaatta agtggagaag
     8281 cattagcagg agaaagaggt tttggtataa ataatgatgt agttaatgat attgctattg
     8341 ctataaaaaa aatacaagaa ataggtgttg aagtagctgt agtagtaggt ggaggaaact
     8401 tctggagagg cagaaccagt gaaggaatgg acagaactac tgctgactat ataggaatgt
     8461 tagctacagt tatgaatgca atggcgctac aggatgcact agaaaatata gatgttgcta
     8521 caagagttca aactgctata gacatgagac aaattgcaga accatatata agaagaagag
     8581 cagttagaca cttagaaaag gaaagagttg ttatatttgg tgcaggaaca ggaaaccctt
     8641 actttacaac agatacaact gctgctttac gtgctgctga gatggaagca gaagttatat
     8701 tgttagctaa aaatgttgat gcagtatatg acaaggatcc aaaagtacat gcagatgcta
     8761 agaaatttac agaattaagt tacatggaag ttatacagaa agaattaaaa gtaatggatt
     8821 ctacagctac atctttatgt atggataata aaattccaat aaaggtattt gagcttacaa
     8881 cagagaatat aataagagca gtaaaaggcg aaaatatagg aactactgta aaataatgaa
     8941 gaatgaagga ggagtaaaat tatgaaactg gaaatacata agcaattaga agagaaaatg
     9001 aatgggacaa ttgatgcttt aaagtttgaa tttggaacta taagagcagg aagagctaat
     9061 gcacaaatgc tagacaaaat aagagttgat tattatggaa ctccaactcc aataaatcaa
     9121 ataggtgcta tatctgtacc agaaccaaga atattaatga tatctccttg ggataaatct
     9181 gctatgcatg aaatagaaaa agcgatagct aactcagact taggattaaa tccatcaaat
     9241 gatggtgagg ttataagact ttctgtacct gctttaacag aagaaagaag aaaagaatta
     9301 gctaaaaaag ctagtaaagc tgcagaagaa tttaaagtta gaataagaaa tgaaagaaga
     9361 gacgccaatg agaaaattaa aaagatggaa aaaggcggag aattaactga agatgaatta
     9421 aaaaaagcac aagatgaagt tcaaaaaatg actgataaat ttataaaaga aatagataca
     9481 ttattatcta aaaaagaaaa ggacataatg gaggtataat attgaaaaaa tattataacc
     9541 ttttaggtct tcatgtagat gaggtaaaac aatattttga tgagcaaaat ataaattata
     9601 ccgtaaaatc tattgaaggt aaaaaggata aagaaaaact tgtagttcct agagttataa
     9661 aaatatctga gataggtgat agtgtagaac tgataatcac atacttttct gactccttag
     9721 tttaaggagt cagaacaata ttggaggcaa aatatgaata acaacattat atatgatata
     9781 gacttaaata acatacctac tcatattgct attataatgg atggaaatgg aagatgggca
     9841 aaagctagat ttcttccaag aactgcagga cataaggctg gagtagaaac tataagagat
     9901 atagtaaaag aatgctcaaa attaggtgtt aagcacttaa cattatatgc attttcaaca
     9961 gaaaactgga aaagaccgaa acttgaagtt gacacactta tgaacttatt atctacatac
    10021 ttaagaaatg aaatagctga acttcaccaa aacaatgtaa aagtaactgc tataggagat
    10081 ataagtgctt taccaaaaac ttgtataaga gaattaaact cagctaagga actgacaaaa
    10141 aacaatacag gagttaactt aaatttagca ttaaactacg gaagtagagc agatataaaa
    10201 aatgctctta tagatatagt aaaaaattgt gaaagtggta aaatagatat aaacaatatt
    10261 gatgaagata ttataaaaaa ctatttaagc acaaagtcta taccagatcc agacttggta
    10321 ataagaacaa gtggtgaaca aagattgagt aactttttac tttgggaagt tgcttattct
    10381 gagttttact ttactgatat acattggcct gattttaaaa aagaagaact acagaaggct
    10441 atatacgtat atcaaagaag ggatagaaga tttggaggac ttaagtaagg agattatttt
    10501 atgcttacaa gaattattgc ttcactagct ctggttccat tatttctatt tgttgtatat
    10561 ggaggtattc ctctatatat agctgagaca gcaatagttt atattgcatt gcatgagttt
    10621 tataaggcat ttaaaataaa agatgtacat ccaatattca ttataggata tttattttct
    10681 atttatttag ctgtaaaaaa tatttttaat ttaccattag aatatactta tgcagtaatt
    10741 tttattttat ttttagcaag tataatttat atgttgatgg gcaaaaacaa tgttatagat
    10801 gtttcgatta cgtttttagg agttttttat ataggagtat ttttagattt tataattata
    10861 actataaatg gatttgaaaa aggtagtata tatgtatggc ttatatttgt gatatctttt
    10921 atgacagata tatttgcata ttttagtggt tatttacttg gaaaacataa gctaattcct
    10981 aaagttagtc ctaaaaagac tatagaagga gctattggag gaataatagg aagtacactt
    11041 tgttgtattt tatttggata tttatttggc attgatttgc ttcaattagc gataatagga
    11101 agtataggaa gtgtaatagc tcaattagga gatttatttg catcttctat aaagagatat
    11161 gtagggataa aggattatgg aaagataata cctggtcatg ggggtatatt agatagattt
    11221 gatagtgtta tattagttgc accttttgta tatagtgcga ttaaattttt tattagataa
    11281 agattggttg gatttaaaag agcaatctat aattgctagt aatgaaaata gctatgtaac
    11341 ttttaaatgc agagatattc tgcagttatt agttacatgg ctattttcat tgttcaaaaa
    11401 tttagaagac cttggggaat tgtataagaa taggtattac tttataatag tatactatat
    11461 ttgtag
//
LOCUS       DAEPIN010000096        11150 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.096, whole genome shotgun sequence.
ACCESSION   DAEPIN010000096 DAEPIN010000000
VERSION     DAEPIN010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11150
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.096"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            391..2886
                     /locus_tag="KR250_003284"
     CDS             391..2886
                     /locus_tag="KR250_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBH0069175.1"
                     /translation="MPENNNQLLEEITLLLNEGKVAINKAENQNLILILGGTGCGKST
                     AINYLAGCVMMEVEDEDTGISYIDCLDPVAEIGSGAVSQTLYPNIVNMRKPIEGMDAK
                     LCDTAGFSDNRGVAHDISAAVSLGEVFKNSASICAVIVLIPEADILDSRLTKVLDLFQ
                     QLNMCLHKENFKGSITFFISKSFLGRTEKQIYNVIKRKYEQLCENLDSNIEEKAWLFE
                     ELLKDNGKNIHLCKPLASDEREGLLKQVLELDEVKDKTSAFQYPISADARLVLDTLIK
                     EIQNNIVSSLEDYVKAYTEDFINIIGSITMIQEIAKYDEVVKVFKNWLESTTSLTIER
                     FINVLAKQSGLFLQDSCMKLQNKIKGNLDIIDALSKYTEHGTGEILLPSVHMEFIIEK
                     ASKKIMEQNFIIIRKNLEKVLVSYEVQKQVFTLPNREKLMEVANKKDYVYSVSDFNSI
                     LTNVTVTQNEELEDSITRLSAYGEEGNEIIREFIGKYILNPFIVKTININDKKGIEIR
                     ALIPNLVISSVLEYFKNRKVDDNVSTFMFFAKDTIYMDDNLGMNFASEKNIILASNNL
                     DVGSNVKINVSGKSGELVDNSMYGKNGEDGRDAGNIYLYIKEEIRNYRLTLIANGGNG
                     SKGIDGENGETGASGSNGADAGYTSYFHGQMFKGYFEKGTSGKKGEKGGAGGNAGRGG
                     NAGKKGNIYFISDSEEQKNRIINHCDITVKDGEAGPNGRPGLGGYGGSGGYHGYNTLS
                     FSPSGIDKTVYYTGYFTSWDYKLDSIIKYHIEFSGGSWNEGKTGPAREYAEYGDRGDN
                     GGFNAPNTKLSTAVSKVEENLIKEKYQGYCSEI"
     gene            2901..8111
                     /locus_tag="KR250_003285"
     CDS             2901..8111
                     /locus_tag="KR250_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HBH0069176.1"
                     /translation="MANKLYSEIVNLLEEGRDELRKYDLKEKSILLFLGASGVGKSTC
                     INYLKGCVMEEKTDEETGQIYITAKDSAVEIGNGVYSKTLCPEVVDIANRDFSLCDCP
                     GFFDNRGAEYMIAGAMLVRETISTSSKVKGMVVILDYQSLLDSRHTLLIETGKNLKDM
                     LGDYAKYKENFFFLINKIPKTLIGNVTEEKIRNILEKAKEDLDSQEKDYDGCKELVYL
                     LNVALSDGVSIKLCNPLDKQPEKLLDEMKNLPETFEKTDLGLMLTASCKIEIQKAVDV
                     TYKDLEKLLVPYIESVKKGMEDSISILSSINSLKDKKIQLDKMYTDLCSRTNNMKEIM
                     EFVQELSYGDGVQKVNILEETRLLLELNSYCSETHIVGLSELLKSLLKNTLEDTLKDI
                     KYWIAEEEYREEEAKKLEDAEYRIEKIQSCTYIKSYEKENVLSIYIPIPKVNLSDLTK
                     IISGYQLNILSSIYMYGDCELKIDCNMEKEVFSGKNIVLLTEKLHIPEGTYSINVSGK
                     DAEKLVGESSIYDGMNGRNGNNAGTSGDIILSFKEIEKRGELKLYVDGGRGGNGQDGK
                     KGDTGITGEAGANAKIGSKKESKEFANVKGTKYYVVKAEKGKQGGTGGTGGNGGNAGL
                     GSKSGQVYLLGKAPLLKSAISKKDGNHGLAGKGGSGGAGGAGGLGGIRGIYEETIRYI
                     GTSPPMDNSDRNYYYFDRYVFNHALNQKQAADDGEKLNALYEEHKDWIISKREDSGSA
                     GAEGTAGHSGDALKEKELLKQEYSLSIDTFFYEFFERFGFSGIKGYRNLRLLCAVGIL
                     EADILELSHIYEIFHAVEKIEMQLNREILQKYYSFEKKNKAVLSLDDMNTKKEFYKEA
                     KEIYEYLINKLMQSDRIESFVGKNQNFSEWSMILFALEQKTAFYVQMIEESEIGGNRV
                     TDIRSLTDYLDKTLRELEQGKKDELKEGYFNTYKESILTQIESGNQQIEVLKNELENK
                     MLSVNAKMNLLIQEIIKMKEDEQEDIEELKKKQAKLEEQMKTQLILSGIQSALSLLTA
                     FSGVGQACLGAFDSAKSLKENLSAKEEEYKWTNRMVNFSDFKLDSDGMRFVLGEQLSE
                     DRKNALDKMTYNLAGVDEERRTKFDQIKEIIVKSIDDDNYADKADDIQRAKEGLSESE
                     YKEIYTKIQKKEIGMLEANYNNKQQKDKLAALEKSKQAIERQQKTSKIKNALSIGSSA
                     CSLINTAISGVNNCLNVKSSFESKIEVVKEAIKDKENNIKKLDELNTQMVEFKDKTLK
                     ESVIPFVEKFDSTVKNQDIFENEISKLKMKEVLTELSTKFGSLKMENSEEISIIFKKV
                     QDMMETQIIITDKIDKKKDNLAMGELIYQMSDSGNQMNEYQKKLIHQIELNQVRYLCQ
                     VEAAAFQLWTFPFGSNIVNILNRCHVGITEDENEVINQAKEQNRQIREFLDQDAYQWQ
                     KTDNHILKVALTGSLDGKSRVGFENTYSLYDCNEEEVKKLLTGDEISFEVPLNKNFDA
                     IKYVTMYANIPNLRRNILEDSLKVKVHIHLYDSGYFSLYDDKKDVYYQYVFPQNYINI
                     THGLDVKYNDSDSINSIWSSVEDKGIEKFKNAGLNSGRSPYSMAKISLKAHFNKKLSY
                     DSLTNEQKKKLESYFDKPSKYAWSDSIDILKSLGYIDFKGILSKSWYEIKKDIDSDGE
                     IMKHFSILQVDLLERIIYEQTSILKENIPNLTIELVGCGIYVESEFNYLKQNDLKCYE
                     ERLVENTIIG"
     gene            complement(8336..10435)
                     /locus_tag="KR250_003286"
     CDS             complement(8336..10435)
                     /locus_tag="KR250_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HBH0069177.1"
                     /translation="MNFYIKHEIRGRIRFDLQLGKLNNKQSDTLLYYLTSINGITKAK
                     VYDRTGDAVIYYNGEREYIIREITKFSFNNSKIENLVPEHTSRELNNYYRDKLANKLL
                     VKSISKIFFPKSLRFFLIGLHSIKYFKKGLSSLLNKKIEVSVLDATAIGISIFRKDIN
                     TAGSVMFLLGIGELLEEWTRKKSIDDLAQSMSLNIEKVWLRKGDTEILIPISEIKEGD
                     LVSVTMGNMIPLDGVIVSGEAMVNQASLTGESLAVNKKEGSYIYAGTVIEQGNIVMCV
                     KEKAGTTRFQKIVTMIEESEKLKSSVESKFEHLADTLVPYSFLGSILTYAITRNPIKS
                     LSILMVDFSCALKLSIPISVLSAMRECNNNNITVKGGKFLEGVACADTIVFDKTGTLT
                     KAQPTVSDIITFQNYNKEDMLRLAACLEEHFPHSIANAVVYEAEKQGLSHKEMHTEVE
                     YIIAHGISTKVDDKKVVIGSKHFVFEDEKCTIPDGEEDKYNNLSDEYSHLFMAISGKL
                     SAVICINDPLRKEAKYVISNLRECGIKKIVMMTGDSEKTAKSIASKVGVDEYYSEVLP
                     EDKANFVKSEKLKGRKVIMIGDGINDSPAISESDVGIAMSEGAEIAREISDITISADN
                     LNNLIVLKQISNKLMKRVDLSSKFIIGFNLGLILLGVGGFVRPSTSAFLHNASTVGIS
                     LNSMTNLLENTNTYNYH"
     gene            complement(10478..10873)
                     /locus_tag="KR250_003287"
     CDS             complement(10478..10873)
                     /locus_tag="KR250_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069178.1"
                     /translation="MNILKMKKTGIFLGGVLFGTVGVKILSSDCVKKTCTRATAGALK
                     VKDSVLETTTKIQENVEDILAEAKEINEQEDLSTSKVNVVQDTLKCEQEVLKTETIKT
                     VEDTNSNSIKCEEIKDNVIEEKNSTCTTE"
BASE COUNT         4244 a         1301 c         2036 g         3569 t
ORIGIN      
        1 ataaaatatt caagctggta taggtgtaaa acttaagcag tagtatagga atgtttaaat
       61 taatggtaca aatataatta aaaatctata tgtaataacc acaggaaaat aatggacata
      121 taatataaaa aacaactgta ttacataaaa tacaataaaa taagcgcatt aaataaaata
      181 actgtgttga taaagattat attatacaac acagttattt taataaacaa agaagaataa
      241 ttattgagtg aaaatacaaa tttagtgaaa atgcaaattt attaaaaata aaaaataaaa
      301 aacaaattta aatatagtat aaaaactttg caatctactt aagggattaa actttattta
      361 aataaaatat ataaccaagg gggaaaataa atgccagaaa ataacaatca acttttagaa
      421 gaaataacac tcttattaaa tgaaggaaaa gtagcaataa ataaggctga aaatcaaaat
      481 ttaattctaa ttcttggtgg gacaggatgt ggcaaaagta ctgcaattaa ctatcttgca
      541 ggatgtgtaa tgatggaagt agaagatgaa gatactggta tatcatatat tgattgctta
      601 gaccctgtgg cagagattgg ttcaggagct gtttctcaaa ctttataccc gaacattgta
      661 aatatgagaa aaccaataga aggaatggat gcaaaattat gtgatactgc aggttttagc
      721 gacaataggg gagtagccca tgatattagt gcagcagttt cattaggtga agtatttaaa
      781 aattctgcat ctatatgtgc agtaattgta ttgataccag aagcagatat attagattct
      841 agacttacaa aagtactaga cctgttccaa cagttaaata tgtgtcttca taaagaaaat
      901 tttaagggta gtataacttt tttcatttca aaaagtttct taggaagaac agaaaaacaa
      961 atatataatg ttataaaaag aaaatatgaa caattatgcg aaaatttaga cagtaatata
     1021 gaggaaaaag cttggttatt tgaagaattg ctaaaagata atggaaaaaa tattcatctt
     1081 tgtaagccac ttgcatcaga tgagagggaa ggcttattaa agcaagtact agaattagat
     1141 gaagttaaag ataagacatc agcattccaa tatccaatta gtgcagatgc aagattagtt
     1201 ttagatacgt tgataaaaga aattcaaaac aatatcgtat ctagcctaga agattatgtt
     1261 aaggcgtata cagaagattt tatcaatatt attggaagta taacaatgat tcaagaaata
     1321 gcaaaatatg atgaggttgt aaaagtattt aaaaactggt tggaaagtac aacaagtctt
     1381 actatagaga gatttatcaa cgtattagcc aaacaaagtg gattattctt acaagatagt
     1441 tgcatgaaat tacaaaataa gataaaagga aacttagata taattgatgc tttaagtaaa
     1501 tatacagaac atggaactgg ggaaatttta ttaccttctg tacatatgga atttattata
     1561 gaaaaggcaa gtaagaagat aatggaacaa aacttcatta taataagaaa gaatttagaa
     1621 aaagttttag tttcttatga agtgcaaaaa caggtattta cattaccaaa tagagaaaaa
     1681 ttaatggaag ttgcaaataa aaaggattat gtatatagtg taagtgattt taattcaatt
     1741 ttgacaaatg ttacagttac acaaaatgaa gagttagaag acagtataac aagattaagt
     1801 gcatatggag aagaaggtaa tgagataatt agagaattta ttggtaaata cattttaaat
     1861 ccatttatag taaaaacaat taatattaat gataagaaag gtattgaaat aagagcatta
     1921 attccaaact tagtaattag cagtgtattg gaatatttca aaaatagaaa agtagatgac
     1981 aatgtttcta catttatgtt ctttgctaaa gatactattt atatggatga taaccttgga
     2041 atgaactttg catcagagaa aaatattatt cttgcttcta acaatcttga tgtaggtagt
     2101 aatgtaaaaa ttaatgtaag tggaaagagc ggagaacttg tagataattc tatgtatggg
     2161 aaaaatggtg aagatggtag agatgcaggt aatatttact tatatattaa agaagaaatt
     2221 agaaattatc gtctaacact gattgcaaat ggaggaaatg gttctaaagg aatagatggg
     2281 gaaaatggtg aaacaggagc ttctggaagt aatggagctg atgcaggata tacaagttat
     2341 ttccacggac agatgtttaa agggtatttt gaaaaaggta caagtggcaa aaaaggtgaa
     2401 aaaggtggag ctggtggaaa tgctggaaga ggtggaaatg ctggtaagaa aggtaatatt
     2461 tacttcattt cagattcaga ggaacaaaaa aatagaataa ttaatcattg tgatataaca
     2521 gtgaaagatg gagaagcagg tccaaatggt agaccaggtt taggtggata tggaggaagt
     2581 ggtggctatc acggatataa tacactttca ttttctccaa gtggtataga taagactgta
     2641 tactatacag gatattttac aagttgggat tataagttag atagtataat taaatatcat
     2701 atagaattta gtggtggaag ctggaatgaa ggtaaaacag gaccagcaag agaatacgct
     2761 gaatatggag atagaggaga caatggagga tttaatgctc caaacactaa gttatcaaca
     2821 gctgtttcaa aggtagaaga gaatcttatt aaagaaaaat atcaaggcta ttgttccgaa
     2881 atatagaaag ggatggatgt atggcaaata aactttatag tgaaatcgta aaccttttgg
     2941 aagaaggaag agacgagctt agaaaatatg acttaaaaga aaagtcaatt ttgttattct
     3001 taggtgcttc aggcgttgga aagagtactt gtataaatta tttaaaaggc tgtgtgatgg
     3061 aagaaaaaac ggatgaagag actggacaaa tatatataac tgcaaaagat tcagctgtcg
     3121 aaattggaaa tggtgtatat tctaagactt tatgtccaga agttgtagat attgcaaata
     3181 gagatttttc attatgtgat tgcccaggtt tttttgataa ccgtggagca gagtatatga
     3241 ttgctggtgc tatgcttgta agggaaacta tttccacatc ttcaaaggta aaagggatgg
     3301 tagttatttt agactatcaa tccctactgg actctagaca tacattattg atagaaacag
     3361 gcaaaaattt gaaagatatg cttggtgatt atgcaaagta taaagaaaat ttcttctttc
     3421 ttatcaataa aatacctaaa acactaattg gtaatgtaac agaagaaaaa ataagaaata
     3481 tattggaaaa agcaaaagaa gacctagata gtcaagagaa agattacgac ggttgtaagg
     3541 aattagtgta cttattaaat gtagcattat cagatggtgt atctattaaa ttatgtaatc
     3601 cactagataa acagccagaa aagttgttgg atgaaatgaa aaatctacct gaaacatttg
     3661 aaaaaacaga tttggggttg atgcttactg cttcttgtaa aatagaaata caaaaagctg
     3721 ttgatgtaac ctataaagac ctagaaaaac ttttagttcc ttatatagaa agtgttaaaa
     3781 aaggtatgga agattctatt tccattctat cttctataaa tagtctaaaa gataaaaaga
     3841 ttcaattaga taagatgtat acagatttat gtagtaggac taataatatg aaagagatta
     3901 tggagtttgt acaagaactt tcatatggtg atggcgtaca gaaagtgaat atattagaag
     3961 aaacacgtct tcttttagag ttaaatagtt actgtagtga gactcatatt gttggtctaa
     4021 gtgagttgtt aaaatcattg ctaaaaaata ctttagaaga tacattaaaa gatatcaaat
     4081 attggatagc agaagaagaa tatagggaag aagaagccaa aaagcttgaa gatgcagagt
     4141 ataggataga gaagattcaa agttgtacat atataaaatc atatgagaaa gaaaatgtac
     4201 tttctatata tattcctata cctaaagtga atttatcgga tttgactaaa ataatatctg
     4261 gttatcaact taatattttg tcatctatat atatgtatgg agattgtgag ttgaaaattg
     4321 actgtaatat ggagaaagaa gtcttctcag gtaagaatat tgtactactt acagaaaaat
     4381 tacatatacc agaagggact tattcaatca acgtatctgg taaggatgca gaaaaattgg
     4441 ttggagaaag ttctatttac gacggaatga atggaagaaa tggcaataat gcaggtactt
     4501 caggtgatat catattatct tttaaagaaa tagaaaaaag aggagaactt aaactttacg
     4561 tagatggtgg acgtggtgga aatggacaag atggaaaaaa aggagataca gggataactg
     4621 gagaagctgg ggcaaatgct aaaataggtt ctaaaaaaga gtcaaaagaa tttgctaatg
     4681 ttaaaggtac aaaatactat gtggtaaaag cagaaaaagg aaaacaaggt ggaactggag
     4741 gcacaggagg taatggtgga aatgcaggtc ttggaagcaa gtctggacag gtttatctcc
     4801 ttggaaaagc tcctttatta aaaagtgcta taagtaaaaa agatggaaat catggtttag
     4861 caggaaaagg aggctctgga ggagctggtg gtgcaggagg tcttggtgga attagaggta
     4921 tatatgaaga aacaataaga tatataggta cctctcctcc aatggataat agtgatagaa
     4981 actactatta ttttgataga tatgtgttta atcatgcttt aaatcagaag caagcagcag
     5041 atgatggaga gaaattaaat gctctttatg aagaacataa ggactggata attagcaaac
     5101 gtgaagattc aggttcagcc ggagcagagg gaacagccgg tcactctgga gatgcactaa
     5161 aagaaaagga attgttaaag caggagtatt ctttgtcaat agatacattc ttctatgaat
     5221 ttttcgagag atttggtttt tctggaatta aaggttatag aaatcttaga ctgttgtgtg
     5281 cagtaggaat tttagaagct gatattttag aactttcaca tatttatgaa atttttcatg
     5341 cagttgagaa aatagagatg caattaaata gagaaatact tcagaagtac tatagttttg
     5401 agaagaaaaa taaagcagta ctttctctgg atgatatgaa tacaaagaaa gaattttata
     5461 aagaagcaaa ggaaatctat gaatacctta taaataaatt gatgcagtct gatagaatag
     5521 aatcttttgt aggaaaaaat cagaatttta gcgaatggag tatgatttta tttgcacttg
     5581 agcagaagac agcattttat gtgcagatga ttgaagaaag tgaaattgga ggaaatcgtg
     5641 ttacagatat tcgttctttg acagattatc tggataagac cttaagagaa ttggaacaag
     5701 ggaagaaaga tgagttaaaa gaaggatatt tcaatacata taaggaaagt atcctaactc
     5761 aaattgaatc tggaaatcaa caaatagagg ttttaaaaaa tgaacttgag aataaaatgc
     5821 tttctgttaa tgcaaaaatg aacctgttga ttcaagaaat tataaagatg aaagaagatg
     5881 aacaagaaga catagaggaa ctaaaaaaga aacaggcaaa acttgaggaa caaatgaaaa
     5941 cacagttaat tctttctgga atacaaagcg cattgagttt actgactgca tttagtggtg
     6001 ttggtcaagc ttgtctaggg gcatttgaca gtgcaaaatc tttgaaggaa aatctttcag
     6061 ctaaagaaga ggaatacaaa tggactaaca ggatggttaa tttttcagat tttaaattgg
     6121 attctgatgg tatgagattt gtacttggag aacagctaag tgaagataga aaaaatgctt
     6181 tagataaaat gacttataat ttggcaggtg ttgatgaaga aagaagaact aaatttgacc
     6241 aaattaagga gattatagtt aagtctatag atgatgataa ttatgcagac aaagctgacg
     6301 atattcaaag agcaaaagag gggttaagtg aaagcgagta taaggaaatt tacacaaaaa
     6361 tccagaaaaa agaaataggt atgttggagg caaattacaa taataaacag caaaaagata
     6421 agttagctgc actagagaaa agtaaacaag ctattgaaag acagcaaaag acatctaaaa
     6481 ttaaaaatgc attgagtatt ggaagttctg cttgttctct tataaatact gcgattagtg
     6541 gtgttaataa ttgtcttaat gtaaaatcta gttttgagag taaaatagag gtagttaaag
     6601 aggcaattaa agataaagaa aataatatta agaaattaga tgaattgaat actcaaatgg
     6661 tggaatttaa agataaaact ttgaaagaat ctgttatacc atttgtagaa aaattcgata
     6721 gtacagttaa aaatcaagat atttttgaaa atgagataag taaattaaag atgaaggaag
     6781 ttttaactga attaagcaca aagtttggaa gcttaaaaat ggaaaattca gaggagattt
     6841 ctataatatt taaaaaagta caggatatga tggaaactca aattatcatt actgataaga
     6901 ttgataagaa aaaagataat cttgcaatgg gagaattgat ttatcaaatg tcagatagtg
     6961 gtaatcagat gaatgaatat cagaaaaaac ttatccatca aattgaatta aatcaggtta
     7021 gatacttgtg tcaggttgag gcagcagctt ttcaactatg gacattccca tttggaagca
     7081 atatagtaaa tatcttaaat agatgccatg tagggattac agaagatgaa aacgaggtta
     7141 taaatcaggc gaaagagcaa aatagacaga ttagagagtt tcttgaccaa gatgcttacc
     7201 aatggcaaaa aacagataac catattttga aggtagcatt aacaggcagt ttagatggga
     7261 aatcacgtgt gggatttgaa aatacatatt ctctatatga ttgtaatgaa gaagaagtga
     7321 aaaagctttt gacaggagat gaaattagtt ttgaagttcc attaaataaa aactttgatg
     7381 ccataaaata tgtaaccatg tatgcaaata tcccgaacct tagacgaaat attttagagg
     7441 atagtttaaa agtaaaggtg catattcatt tatatgatag tggatacttc tctctatatg
     7501 atgataaaaa agatgtctat tatcaatatg tatttccaca aaactatata aatattacac
     7561 atggtcttga tgttaaatac aatgattcag attcaataaa ctcaatatgg tcttcagtag
     7621 aagataaagg aattgaaaag ttcaaaaatg caggattaaa ctctggtaga agtccatatt
     7681 ctatggctaa gatttctttg aaggcacatt ttaataagaa gctttcttat gattcactta
     7741 caaatgagca gaagaagaaa ctagagtcat actttgataa acctagtaag tatgcatgga
     7801 gcgatagtat agatatattg aagagtctag ggtatattga ttttaaggga attttgtcaa
     7861 agagttggta tgaaatcaag aaagatatag atagtgatgg ggagattatg aagcattttt
     7921 ctatattaca agttgattta ttggaaagaa ttatttatga gcagactagt atactaaagg
     7981 aaaatatacc taatttgaca attgaattgg taggctgtgg tatttatgta gagtctgaat
     8041 ttaattatct aaagcaaaat gatttaaaat gctatgaaga aagattggtt gaaaatacaa
     8101 ttattggttg aaaatataat taatatataa attttaaaga aatttacaaa aggggatgtt
     8161 tcaaaatgaa aatttgaatc atctcctttt ttgtaaaaaa tagaaataag ttgagtttaa
     8221 aatttgtaaa gtatgcttta tataattttg attagaaaaa agctaatttt aagacatatt
     8281 tttttaaaaa gatgaattat aaatttttta tatcatatgt tttatgtaat ggagattaat
     8341 ggtagttata agtattggtg ttttccaata aatttgtcat actgtttaaa cttattccga
     8401 cagtagatgc gttgtgtaaa aaggcagatg tacttggtct aacaaatcca cctacaccaa
     8461 gtaaaatcaa acctaaatta aagccgataa taaacttaga acttaaatca actcttttca
     8521 taagcttatt actaatctgc tttaaaacga ttaaattatt caaattatct gctgatatgg
     8581 taatatctga aatctctctt gctatttcag caccttcact cattgcaatt ccgacatctg
     8641 attctgaaat agcaggagaa tcattgattc catcaccaat cataattact ttgcgacctt
     8701 tgagtttttc acttttaaca aaattggctt tgtcttctgg taagacttct gaataatatt
     8761 catcaacacc aactttactt gctatagatt tagctgtttt ttcgctatct ccagtcatca
     8821 tcacaatctt ttttattccg cattctctta aatttgaaat tacatatttt gcttcttttc
     8881 gtaaagggtc atttatgcaa ataactgctg ataatttacc tgatattgcc ataaataggt
     8941 gtgaatactc atctgataga ttattgtact tatcctcctc accgtctggg atagtacact
     9001 tctcatcttc aaatacaaaa tgtttgctac ctattacaac ctttttatca tcaacttttg
     9061 ttgaaatgcc atgagcaata atatattcta cttctgtatg catttctttg tgactaagcc
     9121 cttgtttttc agcttcataa actactgcat ttgctatgga gtgtgggaag tgttcttcta
     9181 agcaagctgc aagtcttaac atatcctcct tattataatt ttggaatgta attatatctg
     9241 aaacagttgg ttgagctttt gttaatgtgc ctgtcttatc aaatacaata gtatctgcac
     9301 atgcaacacc ttcaagaaat tttccacctt ttacagtaat gttgttattg ttacattctc
     9361 tcatggctga tagtactgaa atagggatag agagttttaa tgcacaagag aaatctacca
     9421 ttaatataga gagtgatttt atagggtttc tagtaattgc ataggttaat atacttccta
     9481 aaaagctgta tggaactaat gtatctgcta gatgttcaaa tttactctct acagaagatt
     9541 ttagtttttc agattcctca atcatagtta ctattttctg gaatctagta gtaccagctt
     9601 tttccttgac gcacataaca atattgcctt gctctattac agttccagca tatatgtaag
     9661 atccttcttt tttgttaact gcaagggatt cacctgtaag tgatgcctga ttgaccattg
     9721 cttctcctga tactataact ccatccaatg gaatcatatt tcccatagtt acacttacta
     9781 aatctccttc ctttatctct gaaattggaa ttaggatttc agtgtcacct tttcttaacc
     9841 atactttttc aatgtttagt gacatacttt gagccaagtc gtctatagat ttttttctag
     9901 tccattcttc taatagttca ccgataccaa gaagaaacat aactgaacca gccgtattga
     9961 tatcttttct aaaaatagaa atacctatag cagtggcatc taaaacagaa acttctattt
    10021 ttttatttag cagggaagag agaccttttt taaaatattt tatagaatga agtccaatta
    10081 aaaaaaatct taaggacttt ggaaaaaata ttttggagat acttttgact agcagtttat
    10141 ttgctaattt gtctctataa taattgttta attccctgct ggtatgttca ggaactaggt
    10201 tttctatttt gctattgtta aaactgaatt tagtgatttc tcttattatg tattctcttt
    10261 caccattgta ataaatgact gcatcaccag ttctatcgta aacttttgct ttggtaatac
    10321 catttataga agttaaataa tataagagag tatcggactg cttgttgttt aatttgccaa
    10381 gttgtaagtc aaagcgtata cgacctctta tttcgtgttt tatataaaaa ttcaatttac
    10441 ttcctcctga tattattaat cataataatc attaaatcta ttctgttgta caagtactat
    10501 ttttttcttc aattacgtta tctttgattt cttcacattt aatgctatta gaattagtat
    10561 cttctacagt ttttatagtt tcagttttaa gaacttcttg ttcgcattta agagtatctt
    10621 gtacaacgtt taccttagat gtagataagt cttcttgctc gttgatttct tttgcttcag
    10681 ctaaaatatc ttctacattt tcttgaattt tcgtagttgt ttcaagaaca gaatctttaa
    10741 ctttcaatgc tcctgctgtt gcacgagtgc aagttttttt aacacaatca cttgaaagta
    10801 tcttaacacc tacagtacca aaaagtactc ctcctaaaaa tatccctgtt tttttcattt
    10861 ttaaaatatt cattttacat tatcctttca tttagttaga ttttgtttgt acttatgtat
    10921 aaggtaacaa atgttaaaaa tagagtatgt tttacaaaat tagtttagag agtttagtaa
    10981 ttttatttga tttatattag taaatttaca tgaattttac atatgtagtt tatatagttg
    11041 aaataattct tatatagctt atatatgcag tttatatatc tagtttatat atctagttta
    11101 tatatctagc ttatgtatat aatttacata tgtagtttat atagttgaaa
//
LOCUS       DAEPIN010000097        11082 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.097, whole genome shotgun sequence.
ACCESSION   DAEPIN010000097 DAEPIN010000000
VERSION     DAEPIN010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11082
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.097"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            complement(204..536)
                     /locus_tag="KR250_003288"
     CDS             complement(204..536)
                     /locus_tag="KR250_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HBH0069179.1"
                     /translation="MNTREMKEYRGFYNKKLSETDEFFRVFGSLDDKTYSEGAISKKN
                     KELMGLAISILSRCDECICYHIEGCINSGASKDEIIEAIKIGVIGGGSITYPNARFAI
                     KILEEFNI"
     gene            complement(780..1421)
                     /locus_tag="KR250_003289"
     CDS             complement(780..1421)
                     /locus_tag="KR250_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HBH0069180.1"
                     /translation="MKKAINKQFILSTLICFIPFIVSIYFYNRLPNEVAIHFDNYGNP
                     DNYAPKVIAAFGVPLLMLCIHLYTWFRLENEPKKANFSNVIQNLSKWGVAILSVIIQI
                     MMVLYSTGVSLESNFYTGLILGIIVILFGNYLPKCKRNYSVGIILPWTLNDEDNWNKT
                     HHLAGWIWLIGGILLIINAFINIPFYNIFVIFVIVILPFVYSYLMYKISGKNN"
     gene            complement(1402..1695)
                     /locus_tag="KR250_003290"
     CDS             complement(1402..1695)
                     /locus_tag="KR250_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBH0069181.1"
                     /translation="MGFPETFKALSDPIRREILVMLRENKMSAGEISEKFNMTNATIS
                     YHLSQLKKAGLIFETKYKNFIFYEINISVFEEVMLWFSQFMEKGEKDNEKSDK"
     gene            2088..2138
                     /locus_tag="KR250_003291"
     CDS             2088..2138
                     /locus_tag="KR250_003291"
                     /inference="COORDINATES: protein motif:HMM:NF024729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069182.1"
                     /translation="MTEYIYYYNNKRIKKN"
     gene            complement(2664..3416)
                     /locus_tag="KR250_003292"
     CDS             complement(2664..3416)
                     /locus_tag="KR250_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HBH0069183.1"
                     /translation="MFSVIFLCIGGFLAAFVDSIAGGGGLISMPVLMAIGVPVHLAIG
                     TNKFAASAGCISSAYRYAKSGKINNNLLKKLVPFTIIGSVLGVRCVLSISEEILNVLV
                     VAMILIVAIYTFISKNLGQEDNFEAVNKKNLRLGMLMALIMGFYDGFFGPGTGTFLTF
                     GFIKIYGYDFLHASANTKILNLTSNITSLLLFMINGQVDYKIAIVFALVMIMGAYVGA
                     KVAIKKGSKMIKPIFLVMALFMVVKLVYQTLV"
     gene            complement(3475..5130)
                     /locus_tag="KR250_003293"
     CDS             complement(3475..5130)
                     /locus_tag="KR250_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH0069184.1"
                     /translation="MVDFIESNNEVQKFDWGEVMWIHEPSKTQFNRLSAGIVRFFPGN
                     CQEKHFHLSEEQLLYVIQGEGIQIIDGKKVNIKETSIVYCPPYSEHEIINTGKIDLVI
                     LITYVPHKFSSLRQIPIVFSENNIQELVNVNIIENLANQISNILKLRISVYDLKYKEI
                     FETKKENKFCDICKNIKPCTKKLVKSINNDNFEKVYHCEYGVSSLEIPIVLEENIVGY
                     IECGNFIVYKSIYIDKNLSELSSSSGIDIKYINKIYNEFPLNPKSRLYVLKENLLMMS
                     EFIQEIAKRNFFENQLSIKDEEILKSRKENIRLEEALKKANSRLLEEEYIINPIDMFN
                     NSSKEYPFELELYIEKEIKNMNLEGVYNLIDTNKLRYNDVRDTIQEMIFVLSRTVLRD
                     LEDLKLISYLRNKYNKKISLVNSDEDLWNVLFEFSKECIDKNRAFWRKDKGNLIENIN
                     EYIKKYYKENINLNSISDVFFISPNYLSSIFNERNKVSITEYINLLRIEESKKYLLDR
                     SMSVSDICKKVGFNNSSYFSQIFKKFNSITPNEYRKNMLDNKY"
     gene            complement(6402..7481)
                     /locus_tag="KR250_003294"
     CDS             complement(6402..7481)
                     /locus_tag="KR250_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBH0069185.1"
                     /translation="MILITLCILVAVFVLAFAGLRLIAPTIIRTSHKIEAPGIDRMEM
                     VEIGGIQQALYIRGQNADNPIILWVHGGPGNSMMPFLHLYQYEWENDFTVVNWDQRNV
                     GKTYFANDPAAVLQTMSAERVLQDVHEVTAYLKQEFNKEKIILIGHSWGTVLGTMAVQ
                     TYPEDYSAYIGIAQTVNMVDNERVGYEKTLEMVKAAGKQKDIKALEAIAPYPPAKYGP
                     SYLAALMTVRTYQSKYGLSINMDLGNIISILATPYYSLRELKNTAIVNPNAIDNQGDV
                     LRFLVEEADIRNYGVDYQVPVYYIMGENDYNSPYLPAKKFFDEIVAPDKQIFFIPDAD
                     HLPFLSKKAEFDCVLLEEIAPKLAK"
     gene            complement(7565..8221)
                     /locus_tag="KR250_003295"
     CDS             complement(7565..8221)
                     /locus_tag="KR250_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBH0069186.1"
                     /translation="MTRKNNPKQAIENIITISAKLFAEKGYDKTSMQNIVDASGMSKG
                     GIFHHFSSKEDIFNVVMERRFEQIIETVNQWLGEMHGLTAKEKLRSLVRRHLTDEVIK
                     ESSNMITSAIESPHIILAFTQDNLKKLAPILANVLREGIEDRSIITEFPDECAEVILL
                     LFNFWCDTDVFQGDFPTLRKRFQFLQFLMRQIGVDILEDEIIESINIFYERKPMGQVE
                     "
     gene            8543..8749
                     /locus_tag="KR250_003296"
     CDS             8543..8749
                     /locus_tag="KR250_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069187.1"
                     /translation="MISHAAEICKKYKMPFIIFPTIEKKSIEEWVNIGANMLCFAQDT
                     TFITYVINQLLSFYNEIVPIQKQH"
     gene            complement(8902..9330)
                     /locus_tag="KR250_003297"
     CDS             complement(8902..9330)
                     /locus_tag="KR250_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HBH0069188.1"
                     /translation="MSKIYIVYWSGTGNTEKMANFVAEGVKLKGKTPEVLDVSLLKPS
                     DLKEEDKFALGCPSMGAEQLEEGYMEPFVSELESMVSGKQIGLFGSYGWGNCEWMRDW
                     EERMQNAGATIIGGEGITAMEDPNEEAKDECIELGKTLAE"
     gene            complement(9806..10744)
                     /locus_tag="KR250_003298"
     CDS             complement(9806..10744)
                     /locus_tag="KR250_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HBH0069189.1"
                     /translation="MNKRMKLIPYEINENLRGAKNKFPYGIKQMNARGMWDEGYTGKN
                     IVVGIIDTGCDISHPLLKGKIIGGANFSDDSNGNKNIYEDFNGHGTHVAGIIAASNYN
                     NEVMGVAPDCKLLIAKALNKDGTGTYQSIINAINFAVNNKVDIISMSLGGNKDDKNLK
                     NAVMQAVKNNISVVCAAGNNGDGDSSTSEYSYPASYAEVIEVGAINENYLVEKFSNSN
                     TTIDLVAPGRNIISTYMDNKLAIMSGTSMSAPYVSGSLALIKEWAREEFERDLDEAEL
                     YAQLIKCTRALGIPRTEQGNGYLYLNLYKYKNNSKR"
BASE COUNT         3707 a         1944 c         1156 g         4275 t
ORIGIN      
        1 ataaaagatt taataatgac aatatactat tctcaataca atctatgatg taaataattt
       61 aaaactacta tattatttaa aaatttatat ttatatatgc aattctaaaa acttaataag
      121 aagtgaactt agaaaataaa gcttaatttc aaaggcttaa atagactatt attattagtc
      181 taaataaact acgtgtatca aaattatata ttaaattctt ctaaaatttt tattgcaaat
      241 cttgcgtttg gatatgtaat agaccctcca ccaattacac ctatttttat agcttctata
      301 atttcatctt tacttgctcc agaattaata caaccctcaa tatgataaca aatacactca
      361 tcacatcggc ttaaaattga aatagctagt cccattaatt ccttattttt tttacttata
      421 gccccttctg aataagtctt atcatcaagg cttccaaata ctctaaaaaa ctcatctgtt
      481 tcacttaatt ttttattgta aaatccccta tattctttca tttctctagt attcatcttc
      541 tacctcctat ttttttctta taagagtatc acactgtact tatattttca atattaaata
      601 caaattttca ttttatgttc attagcctaa tttatattat tttttgaaat ctcaataagt
      661 tatcatataa gtaagatttt ttcagaacta aaatagaaat ctcagtaatt tctttattat
      721 ctatctcagt tctgaaatat attattctta tccaaacatt attatagtga ttaattatat
      781 tagttatttt tacctgaaat tttatacatc aaataagaat atacaaatgg tagtattaca
      841 attacaaaga ttacaaagat attataaaaa ggaatattaa taaatgcatt aataattaat
      901 aatattccac caataagcca aatccatcca gctaaatggt gtgtcttatt ccaattatct
      961 tcatcattaa gagtccatgg taaaattatt cctactgagt aatttcgttt acatttagga
     1021 agatagtttc caaataaaat aactattatt cctaaaatta atcctgtata aaaattagac
     1081 tccaaactaa ctcctgttga atataaaacc atcattattt gtataattac tgaaagtata
     1141 gcaactcccc attttgataa attttgtata acatttgaaa aattagcttt cttaggctca
     1201 ttctcaagtc taaaccatgt atacaaatgg atacatagca ttaacaatgg tacaccaaaa
     1261 gctgctataa cctttggagc ataattatct ggatttccat aattatcaaa atgaattgca
     1321 acttcatttg gtaatctatt ataaaaatat attgatacaa taaatggtat aaaacatatt
     1381 aaagtagata aaataaattg tttatttatc gcttttttca ttatcttttt ctcccttctc
     1441 cataaattgc gaaaaccaaa gcataacctc ttcgaaaact gaaatattta tttcgtagaa
     1501 aataaagttc ttatactttg tttcaaatat gagtcctgcc ttttttagtt gagatagatg
     1561 ataagaaatt gtagcattcg tcatattaaa tttttcacta atttctcctg ctgacatttt
     1621 attctccctc aacataacca atatttctct tcttataggg tctgaaagag ctttgaatgt
     1681 ttctggaaac cccaacacca tcacctcgct atttagaatt tcatctaaat agaatataac
     1741 ataacatcca ttttatttca ataactattt agaatttttt ctaaatagtt atttctaaat
     1801 atttattgaa aattttcata ttaagattct caatttactt aagtcgcttt tatttaatct
     1861 tactttatct aattttttta acttaacaaa agtgtattgc accataattg ttaaagcaaa
     1921 aacctaatga ctgaggtact tatatatata acaatacttt taattttaaa ttaaaagtag
     1981 ctaaacccaa tttaaaaagt agttaagtcc tatttaaata gaaaagaatg aaacgagaaa
     2041 tatactgtat aaaagtacat aaaaacatct actaattaga tacaactata acagaatata
     2101 tttattatta taataacaaa agaataaaaa aaaattaatt tagcatagcc ttgttaaata
     2161 tagatttgaa tatgaattta aagttatgta aaattaaaaa tacaggaatg aaaattccat
     2221 tcctgtataa aatctaactt ttgagtctca gtacaatctt attttgagac aatctttttt
     2281 gttttatatt ttcttatatt taaaatataa cgcatatctt atgcaacata ttcatatttt
     2341 tctttatatt ctttcatcgc atcattccaa gcgtcttgta ctgttacacc tcttttatta
     2401 gcaatttttt gtgccagttc aattatcttc atagctcttc ttgatatgtt cataaacatt
     2461 cctcctacaa attaccatat atttcatata tataaatcaa ggttatattt ttatgatata
     2521 taatccttct attgtaagat tacagtttat atgtgtatta tatatgaact atattagaag
     2581 ataattagaa tttttataaa gctatatgat tagattaaat ataaaagctt tacaattact
     2641 tataaattta aaaagtctat aaactaaact aatgtttgat ataccaattt tacgaccata
     2701 aataatgcca ttactaaaaa tattggtttt atcatctttg aacctttctt tatagcaact
     2761 ttagcaccta cataagctcc cattatcatc actaatgcaa acactatagc aattttataa
     2821 tctacttgac catttatcat gaataataac aatgaagtta tattacttgt taagtttaat
     2881 atttttgtat ttgcagaagc atgtaaaaaa tcatatccat atatttttat aaatccaaaa
     2941 gtcaaaaaag ttcctgtacc tggaccaaaa aaaccatcat aaaaacccat tataagagcc
     3001 ataagcatcc ctagtcttaa atttttctta ttaacagctt caaaattatc ttcttgacct
     3061 aaatttttag aaataaaggt gtaaatagcc actattaaaa tcatagccac aaccaaaaca
     3121 tttaatatct cttcacttat tgataataca catctcacac ctagaacact accaataata
     3181 gtaaatggta ctaatttttt taaaaggttg ttattaattt ttccagactt tgcatatcta
     3241 taggcactac ttatacatcc tgctgatgct gcaaatttat ttgtaccaat ggcaagatgt
     3301 actggcactc caattgccat aagaacaggc atgcttataa gtcctcctcc tcctgctata
     3361 gaatctacaa aagcagctaa aaacccccct atacataaaa aaataactga aaacataaaa
     3421 ttcctccatc attctctttc aatttttctt acaatatata ttatataatc ttatttaata
     3481 cttattatcc agcatattct ttctatattc atttggagtt atactattaa acttcttaaa
     3541 tatttgtgaa aaataactac tgttattaaa acctactttc ttacaaatat cactaacact
     3601 catgcttcta tctaacagat attttttaga ttcttctatc ctaagaagat ttatatactc
     3661 agttattgaa actttatttc tttcattaaa aatagaactc aaataatttg gacttataaa
     3721 aaaaacatct gatatactat tcaaatttat attttcttta taatattttt ttatatattc
     3781 gtttatattt tcaattagat ttcctttatc ttttctccaa aatgctctat tcttatcaat
     3841 acactctttg gaaaattcaa ataatacatt ccataaatct tcatcactgt ttacaagtga
     3901 tattttttta ttatatttat ttcttaaata agatataagt tttaaatcct ctaaatctct
     3961 aagcactgtt ctagacaata caaatatcat ttcctgaata gtatctctaa catcattata
     4021 tcttaatttg tttgtatcta ttagattata aacaccttct aaattcatat ttttaatttc
     4081 tttttcaata taaagttcta attcaaaggg atattctttt gaagaattat tgaacatatc
     4141 tatcggatta ataatatact cttcttctaa taatctacta tttgcttttt taagtgcctc
     4201 ttcaagtctt atattttctt ttctactctt caatatctcc tcatctttta tactaagttg
     4261 attttcaaaa aaattcctct tagctatttc ctgaataaat tcagacatca ttaataaatt
     4321 ttcttttaat acatataatc tacttttagg atttaatgga aattcattgt aaattttgtt
     4381 tatgtatttt atatcaatac cagaactact tgataattca gataaatttt tatctatata
     4441 aatagactta tatactataa aattaccaca ttctatatat ccaactatat tttcttctaa
     4501 tactataggt atctctaaac tggaaacacc atactcacaa tgatacactt tttcaaagtt
     4561 atcattattt atgcttttaa caagtttttt agtacacggt tttatatttt tacaaatatc
     4621 gcaaaattta ttttctttct tagtctcaaa tatttcttta tactttaagt catatacaga
     4681 tattctaagc ttcaatatat tagatatttg atttgcaaga ttttcaatta tgtttacatt
     4741 aactaactcc tgtatattgt tttcagaaaa tactataggt atttgtctca aactcgaaaa
     4801 tttatgagga acataagtaa ttaatattac taaatcaatt ttaccagtgt ttataatttc
     4861 atgttctgaa tatggtgggc aataaactat agatgtttcc tttatattga cctttttacc
     4921 atcaattatt tgtattcctt ctccctgaat cacatataaa agctgttctt cacttaaatg
     4981 aaaatgcttt tcttggcagt ttcccggaaa aaatcttaca attccagcac taagtctatt
     5041 gaattgtgtt tttgatggtt cgtgtatcca cataacctct ccccaatcaa atttttgtac
     5101 ttcattattt gactcaataa aatccaccat aaactcacct cgtagttttt gtctaaatat
     5161 tttttcttcc cttcttatat taaaataata tcatcatcat taactttcat tactataaaa
     5221 tcatcatact cttttaatac tcctgtaatt ttagttgcac ttagtttact acaattcagt
     5281 tttttaatat attgataaat tctataatta cttttttatt ttgtaaaatc agtgttttcc
     5341 caaaataatt atatctataa atgaaagtat ttttactgct tacatcaata tatgttatca
     5401 taattttcct ttttattgca tatataaata actgtatata acattgtcca atactatata
     5461 gaaatacatc taagaaagaa tattattaaa ttgttccaat taatattatt tttcttatag
     5521 aaataaaaaa ctgttggatt cttttagaaa aattattttt tacatacctt ttttgcttaa
     5581 atagtctatc ttctcagcta ttttgtatat acactcttct agttcatgat tttattttaa
     5641 tatcttgctt atattgctga aataattaaa ttcttttatc tatcatattg tctcttatat
     5701 cacaaacata ttgtttctta tatcacaaaa atgaaagcat tcttatatct tcaatatata
     5761 aacctgcttt ttttaatctt gtaataaata taggatactc tatattctct tcctagaaat
     5821 aactaatacc acttattttt ctatcatata gaaagtaata aatctttttc ttaataaaat
     5881 tcaatagtaa gtggaataaa attttatatg aataagaaca ttataaaaat actttataca
     5941 atagtcaata aaagatatta gcttcaaatt gtgttgaagt tgtattattt aaagtggaaa
     6001 agcaaataaa aaagaaataa tggtataagt agaaaaatat acttaaaaca aggcgaagcc
     6061 tctcttgtat ctaaatcaag tagagaatta aatctaaaaa agaaaataaa tataaaagat
     6121 gactcctgta cattacaaga atcatcttat ggtttcataa tcactccatg gtttttaact
     6181 gtctgatata aaggtctcag ttcactcata gcatgaccac cttttccgat tgctgacccc
     6241 gtcacttatg gacaaaatgt ccataagtga tagtcctctc ttaaaaccct ctaaatagat
     6301 atatctcaag cacctcattg aaaccagttg ctggaaacaa actgaagcgt tctggaatag
     6361 taaaagaaat tgccaccata tccacgttac tgtcctagtt gttactttgc taacttcgga
     6421 gcgatttcct ctaacaatac gcagtcaaat tctgcctttt tgctaagaaa tggcagatgg
     6481 tctgcatctg gtataaaaaa aatctgtttg tctggcgcca ctatttcatc aaaaaatttc
     6541 ttcgctggaa gataggggct attatagtca ttttctccca tgatgtagta tacagggacc
     6601 tgatagtcaa ctccataatt tcttatgtca gcttcttcca ctaaaaaccg caatacatca
     6661 ccttgattgt caatcgcatt tggattgaca atggccgtat tttttaactc tctcaatgaa
     6721 taatatgggg ttgccagtat actgattata ttgcccaaat ccatatttat agaaagccca
     6781 tacttgcttt ggtatgttct tactgtcata agagctgcca aatagcttgg accatatttt
     6841 gctggcggat atggtgcaat agcttccagc gccttgatat ctttttgctt ccctgcagct
     6901 ttcaccattt caagtgtctt ttcatagccc acgcgctcat tgtccaccat attaacagtc
     6961 tgcgcaatcc caatgtacgc gctgtaatcc tccggatagg tttgcacagc catagttccc
     7021 agcactgttc cccagctatg acctataagg ataattttct ccttgttgaa ctcttgcttc
     7081 aaatatgctg tcacctcatg gacgtcttgc agaacacgct ccgcgctcat ggtctgcaaa
     7141 actgctgccg gatcgttcgc aaagtatgtt ttgcctacgt ttcgctgatc ccagttcacc
     7201 acagtaaaat cattttccca ctcatactgg tacagatgta aaaatggcat cattgaatta
     7261 ccaggacccc catgaaccca aaggatgatg gggttgtctg cgttttgtcc ccggatatat
     7321 aacgcctgct gtatgccgcc gatctccacc atttccatgc ggtcgattcc aggagcttct
     7381 attttatggc ttgtacgtat aatcgtgggg gctatcagac gcaaaccggc aaacgccaga
     7441 acaaaaacag caactagtat gcaaagtgtg attaatatca ttttaagtac cttctttcat
     7501 gaattttttt agccatagat atctcctcta acattaaaaa aagtatgttg ctattttagt
     7561 tttgttactc tacctgtccc attggtttcc tttcataaaa aatattaatt gattctataa
     7621 tctcatcttc caggatatca actcctattt gtctcataag aaactgtaag aattgaaacc
     7681 gtttacgaag tgtaggaaag tccccctgaa atacatctgt atcacaccaa aagttaaaga
     7741 gcagcaaaat tacctctgcg cattcatctg ggaactctgt gatgatgctt ctatcctcta
     7801 tgccttctcg taaaacattt gctaatattg gtgctagttt tttcaaatta tcctgtgtaa
     7861 atgccagtat gatatgcgga ctttctatcg cgcttgtaat catgttacta gattctttta
     7921 tcacctcatc cgttaaatgc cgcctaacta aacttctaag cttctctttt gctgtaagtc
     7981 catgcatttc accgagccat tgatttactg tctctattat ctgttcaaag cgcctttcca
     8041 tgaccacatt aaaaatatct tcttttgaac tgaagtgatg aaaaattccg ccctttgaca
     8101 ttcccgaagc atcaacaata ttttgcatac ttgttttgtc gtatcctttt tcagcaaaca
     8161 actttgcaga aatggtaatg atattttcaa tagcttgctt tggattgttc tttctagtca
     8221 ttatataact ccttcttatt aaaaaccgac tgttggtttt tattatataa gctattcctg
     8281 cttctgtcaa gtaacattgt gtttatggca aaacaataag gtttggtggt tagacaggta
     8341 ttaattagac taatgcgact ttcccctaga tggaaccctt ggtaaatttg caatctttac
     8401 cggctttgat tatatctgta agcaaggcag gtatggccac gcctgccctg tttgaccctc
     8461 ggatattcat tcctatccct cctctcctta gccattacac caaaacgccg ctcattgtct
     8521 ccaatggcgg cgttttaaag atatgatttc tcatgctgct gaaatatgca aaaaatataa
     8581 gatgcccttt attatttttc ctacaataga aaagaaaagt attgaagaat gggttaatat
     8641 tggagcaaat atgctttgtt ttgcacaaga tactaccttt atcacttatg ttatcaatca
     8701 attattatct ttttataatg agattgtacc aattcaaaaa caacattaaa ttataaatct
     8761 aattttatat taaaagagac taatatttta tctatctcta ttaaggatat tttcttatgt
     8821 atcataatat tagtctctct tattattata tactgctatc atcattagtt gttatttata
     8881 ctatttttat aatatacaaa tttattcagc taacgttttg cctaattcta tacactcatc
     8941 ttttgcttct tcatttgggt cttccattgc tgtaattcct tctccaccaa taattgtagc
     9001 accagcattt tgcatacgtt cttcccagtc tctcatccat tcacaatttc cccatccata
     9061 tgaaccaaat aatccaatct gtttacctga taccatagat tctaattctg aaacaaatgg
     9121 ctccatatac ccctcttcta gttgctctgc tcccatagat gggcaaccta atgcaaattt
     9181 atcttcttct tttaaatcac ttggtttcag taagctcaca tctaaaactt ctggtgtctt
     9241 accttttaac ttcacacctt cagccacaaa atttgccatt ttttcagtat ttcctgttcc
     9301 actccaataa acaatatata ttttactcat ttttacctcc tgtagtattt tttaattaat
     9361 caatagttag ttatagctaa ctaacatcta aaaaacaaaa gatttcttta tctgaaatct
     9421 ctttattttt ctaaatatag aatatcatag ttgatattga aattcaatac ttattgataa
     9481 tatttatcat ttatatttta tattcatttt ataatatcaa aacactgtat cgtatttcta
     9541 atctttcata gaaaaatttc taacttatag tacttttgca tctcttataa atcttttata
     9601 tgtataagta catagaaaaa cacacctttt ccagatgtgc tatctataat atcgtatatt
     9661 tatttttcaa ttctaactag ctaatatgtt tataaatttt atcacttaat acctttatac
     9721 attgatgtaa aatcttacac ctaagtaatt tttattattt gtctcgactc tatataatat
     9781 agctatttta tcatcaaatc aaaaattatc ttttactatt attcttgtat ttatagagat
     9841 ttaaatataa gtatccattt ccttgttccg ttctaggtat tccaagcgct ctcgtacatt
     9901 ttattaattg tgcatacagt tcagcttcat ctaaatctct ttcaaactcc tctcttgccc
     9961 attctttaat tagtgctaat gagcctgata cgtatggtgc actcatacta gtaccactca
    10021 taatagcaag tttattatcc atataagttg atataatatt tcttcctgga gctaccaaat
    10081 ctattgtagt atttgaatta ctaaactttt caaccaaata gttctcattt attgcaccta
    10141 cttctattac ctcagcataa ctggctggat aactatactc acttgtacta gaatcaccat
    10201 ctccattatt acctgctgca cacactacag aaatattatt ttttactgct tgcatgacag
    10261 cattttttaa attcttatca tctttgtttc ccccaagaga catagatata atatcaacct
    10321 tgttatttac agcaaagtta atagcattaa ttatactttg atatgttccg gtaccatctt
    10381 tatttaatgc ttttgctatt aataatttac aatctggagc tactcccata acttcattat
    10441 tataattaga tgcagctata atacccgcca catgagttcc atgaccatta aaatcctcat
    10501 atatattttt atttccatta ctgtcatcac taaaatttgc accaccaatt atttttcctt
    10561 ttaaaagagg atgagatata tcacaacctg tatctattat accaactaca atatttttac
    10621 cagtataacc ttcatcccac attcccctag cattcatttg ttttattcca tatgggaatt
    10681 tattttttgc acctcttaga ttttcattta tttcatatgg aattagtttc attcttttat
    10741 tcatcatata tcctcctaaa aattttattg cttataaaaa tcatgaattt tattttataa
    10801 taggtttata aatcaaatta aaacctatat ctacttattt atatttatga tatatttttt
    10861 atttttagga gtcatatttt attgtatata aaataatatt tagataacta tattaaaaaa
    10921 tacatgtttg cttatataca aaacttacta aaatatcttc aatatttttg tatacaaaca
    10981 ttatattttt tatataaaaa tctcataaaa attttgatta tattttcata agttaacaaa
    11041 tgtgggtata atatgaattt acaaaattcc tctgtttgta ta
//
LOCUS       DAEPIN010000098        10941 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.098, whole genome shotgun sequence.
ACCESSION   DAEPIN010000098 DAEPIN010000000
VERSION     DAEPIN010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10941
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.098"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            196..1896
                     /locus_tag="KR250_003299"
     CDS             196..1896
                     /locus_tag="KR250_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069190.1"
                     /translation="MKRYLKTYGVALIIVIIYAFIKLPVLRLDFTSLFSVGIIFFVVA
                     GILDMMLDRNERASKMAKYNFSIAIALLVYIVVVPFITSTPVLHAKSYRELLGKVTES
                     KFTDDISPVSVNDIRLVDEDMAMKLGDKKLGEVPAIGSVSKLGKFHIQNVDGELYWVA
                     PLVHRDIIKWITSLSGTSGYVKVSASNPQDVQLVQEIDGKPIKIVYQPEAYLHQDLQR
                     HLYIHGIVNVGMTDFTLEINDEGRPYWVVSLYEHKIGYGGANATGIATVDAETGKINV
                     YDVKNTPKWVDRIQPQSFVTDQIKDWGVYVNGFLNSVISEKGVLVPTEGTSLVYGNDN
                     RSYWYTGITSSGGDESTIGFMLVDSRTKEARLYKQPGATETAAMKSAEGNVQEKNYEA
                     TFPVMYNILGQPTYVSSLKDKAGLVKRVAFVSVEDYNVLGIGEDKNEALRNYKDALES
                     KGNDLKLDNDLKDEVLEGTVTRISPDVRGGNTNYYVTLDSNKDIIFRATSKVSSELPL
                     TQVGDKVKISYSKEESGVIEMSEFDNLNIANFEDKKAKEKTGTDENTDSKDSENQGNV
                     "
     gene            2066..2683
                     /locus_tag="KR250_003300"
     CDS             2066..2683
                     /locus_tag="KR250_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069191.1"
                     /translation="MTNMEKEILLLISRVRCVTESQFRKIYGDQKRYNKKNFKKTLRK
                     MCSEYTLKKYPCDVDYYNYKDLSYVYYLNGSKLFKGDDLIKALVGSELVVRINTAGCD
                     VKRFYRNVSVDDVKYDLFIEYTDQFGNIKQVLVDVDLNNEIDISKYENLSKKIDKSTI
                     PFFKVPNVLVVTPDNLSELNDLERYDNEYSINFIDLGLNKLFNCI"
     gene            3044..4237
                     /locus_tag="KR250_003301"
     CDS             3044..4237
                     /locus_tag="KR250_003301"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HBH0069192.1"
                     /translation="MSIIVQKYGGSSVADTEKIKSIAENIIERRKENPQMVIVVSAMG
                     KSTDEYITLAKELSNEPSKRELDALMSTGEMISASLLSIALNALGCKAISYNAYQLNI
                     KTSGLHGKSQIDDINVNRIKNSLDEGNVVIVTGFQGINEDGDVTTLGRGGSDTSAVAL
                     AVKLNGKCEIYTDVDGIYFTDPRKYSKASKLDEIEYEEMLELASLGAQVMHSRSIELA
                     QKYGVEIYVGRTCGTEKGTYIRGGKDMKLEDKVITGLATSDDDSSITIKDFKAENISS
                     LFEDIATIGISVDMISQTAPILDKISVSFTVPKEELGECKKIVSKYTDEEHVVIDNNI
                     TKFSLVGLGMKNTSGVAAKVFKIFNENGIMIKLITTSEIRITCAINSDDKQVAIEKIA
                     EVFNI"
     gene            4255..5547
                     /gene="lysA"
                     /locus_tag="KR250_003302"
     CDS             4255..5547
                     /gene="lysA"
                     /locus_tag="KR250_003302"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HBH0069193.1"
                     /translation="MKLHGTMTVNNDELYIGGVSCLELSKKYQTPLYVFDEALVRQNC
                     REYVEYFKAREGKNKVAYAGKAFLPLYMCNLINEEQMCLDVVSGGELYTAYKSNFPME
                     KVFFHGNNKTKEEINMGLDLGVGKFVVDNFYELDLLEALCEDKGKTQEIYFRITPGIE
                     AHTHDYIKTGQIDSKFGFALVNGDLFRAIEKLSEYKNIKLVGLHAHIGSQIFDVEPYI
                     DTVDIMMNLVKEIKEKFNIQLTEIDLGGGVGVYYSEGDKPKTIKEFCETIITKAETSC
                     KELGIEVPTLVIEPGRSIVANAGSTLYTVGSIKDIKDVRVYVSVDGGMTDNVRPSLYQ
                     ASYECSIVNKINHEAMNHVTIAGKCCESGDILIGDINIMDIKSGDILITTSTGAYGYS
                     MSSNYNKIPRAAVVSVFDGNDKIICKRQSYEDLLALEI"
     gene            5764..6423
                     /locus_tag="KR250_003303"
     CDS             5764..6423
                     /locus_tag="KR250_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069194.1"
                     /translation="MRGRVILLISILSIFLVGCSFNKKDEINLVEQGKTYLEKHNYKK
                     AMESLSSALEEDSTNENARAMYMQAMRMSNMTEFEELKNYEGAIKELKLIEKIKNGSS
                     VIKEEATKKKEELTKLEKEQNEAEEQRKKKAKDTAGEDRYRVESDARKSSYSGSSKNN
                     KSSSKNNDKKSDSNKSDNSNSSSQNQEKPNNSNPTPAPTPQDTTGGSDSGGNSQNSNN
                     Q"
     gene            complement(6578..7663)
                     /locus_tag="KR250_003304"
     CDS             complement(6578..7663)
                     /locus_tag="KR250_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBH0069195.1"
                     /translation="MIIISKENIKYYLIVVFIGILFFKFINTPSDFISSIEGFLKFFS
                     PFLLAILLALLLNPLVMLFEIKFKAHRLLAIFLSYIFIGFILAFAIRLLIPSIANTLN
                     RLINEMPMYTDYIDSFIEKNMSNIYFLKTLIPHIQHSLDNLLKEASNFVGKIPKNFLI
                     YTLSISSMLFNMTMGFILSIYIIYDKEKIALGFKRFLYSSTARNKADNIIEFFRTTHD
                     IFYDYLLGRILDSLIIGIIAFLGFQFVIRIENALFLASIIFLGNIIPYFGPFIGAIPP
                     IAMTILYSPQKTIWVIAFLFILQQLDGNFIEPKVMGNQVGIGAIWVISAILVGQSLFG
                     FIGVFLSVPIAAVVKTYVDKYIDNRLQ"
     gene            7854..8570
                     /locus_tag="KR250_003305"
     CDS             7854..8570
                     /locus_tag="KR250_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HBH0069196.1"
                     /translation="MRINKYIASCGIASRRKAEEIILGGRIKVNGSVVKELSFNIDEE
                     KDIVEFDNKKVKPSENYIYIVLNKPEGYITTVKDQFNRPSVIDILKDVRERVYPIGRL
                     DYETSGLLILTNDGDLTYKLTHPKHEIDKMYVASVKGIISDDEIRQFETGLKIEDYTT
                     APAKIRVTKENKEKNYSVCEITIHEGRNRQVRKMCKAINHPVLNLRRISVGKIVLKDT
                     KVGEYRYLTEDEIKYLKSIK"
     gene            8628..9206
                     /locus_tag="KR250_003306"
     CDS             8628..9206
                     /locus_tag="KR250_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBH0069197.1"
                     /translation="MAYAKYLTKITDINKFYLENIINEGDIVIDATMGNGYDTRYLAE
                     KVGEKGFVYAFDIQEEAIKSTRKKLEKEGYTDRVKLIFDGHEKMNQYIKEEVSCVLFN
                     LGYLPRAKHSIITKPDTTLKAIKLSLEMLKENGVISIAIYTGHDGGQEEKDCIYNLVN
                     NLNQDEFNVLESKFLNQVNNPPQLILIEKKKA"
     gene            9600..10472
                     /locus_tag="KR250_003307"
     CDS             9600..10472
                     /locus_tag="KR250_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBH0069198.1"
                     /translation="MEETNEMKDSKHLISNIQSQYTRLSKGQKLIAQYILNNYDKVAF
                     MTACKLGETVGVSESTVVRFANALGYSGYPKLQAALQELIKNKLTTVQRVEMAHDYSD
                     DFAILNKVLKSDIDNIRSTLEEIDERAFKEASNKLLRARKIYILGMRSSFVVAQYLGF
                     YLDIILDNVHIIRMDMGDAFEQIVRINEEDVIVAISFPRYSKKSYQIVNYAKEKGAHV
                     ISLTDSLFAPVASLADNTLLVKSNMASFVDSLVPALSISNALAISVGMKEKEDIKQHF
                     DDLEQIWKRYSVYE"
BASE COUNT         4509 a         1132 c         1894 g         3406 t
ORIGIN      
        1 taggtgcggg atggatagta caaacagtgt ataaaaagta tttatacata cacgtataat
       61 gttaaatata gttgaataag cagaactata aatatataaa gatatcttaa tatataataa
      121 atatgataaa tatttatttt ttgtaaatat atggatataa tagtaataat aacatttttt
      181 agagaggagt attaaatgaa acgatatctt aaaacatatg gagttgcatt aataattgta
      241 ataatttatg catttattaa attacctgta ctaagattag attttacatc tttattttca
      301 gtaggaataa ttttctttgt tgtagcagga atactagata tgatgttaga tagaaatgaa
      361 agagcatcta aaatggctaa atataatttt tcaatagcaa tagctttgct tgtgtatatt
      421 gtagtagttc cctttataac ttctacacct gtacttcatg caaaatcata tagagaacta
      481 ttgggaaaag taactgaaag taaatttact gatgacatta gccctgtcag tgtaaatgac
      541 attcgtcttg tagatgaaga tatggcaatg aaattaggtg acaaaaagct tggagaagtt
      601 cctgcaatag ggagtgtatc caaattagga aaatttcata tacaaaatgt agatggagag
      661 ttgtactggg tagcgcctct tgttcacaga gacataatta aatggataac atctctaagt
      721 ggtacaagtg gttatgtaaa agtatcagct agtaatcctc aagacgtcca attagttcaa
      781 gaaatagatg gaaagccaat aaagatagtt taccaaccag aagcctactt acatcaggat
      841 ttacaaagac atttatacat acatggtatt gtaaatgttg gtatgacaga tttcactctt
      901 gaaataaatg atgaaggaag accttattgg gttgtaagtt tatatgaaca taaaattggc
      961 tatggaggag caaatgcaac aggaatagcc actgttgatg cagaaactgg aaaaataaat
     1021 gtttatgatg ttaaaaacac gcctaaatgg gtggatagaa tacaaccaca aagctttgta
     1081 acagaccaaa ttaaagactg gggtgtttat gttaatggat ttttaaactc tgttatatca
     1141 gaaaagggag ttttagtacc aacagaaggt acatcactag tttatggcaa tgataacagg
     1201 tcttattggt atacaggtat aacatcttca ggtggagatg aatctacaat tggatttatg
     1261 ttagttgatt ctagaacaaa agaagctaga ttatataaac aaccaggagc tactgaaacg
     1321 gctgcaatga aatcagctga aggtaatgtt caagaaaaaa attatgaagc gacgttccct
     1381 gtaatgtata atatattggg tcagccaaca tatgtatcat cattaaaaga taaggctggt
     1441 cttgttaaga gagttgcatt tgtatctgtt gaagattata atgtacttgg aataggtgaa
     1501 gataagaatg aagcattaag aaactataag gatgcattag aatctaaggg taatgattta
     1561 aaacttgata atgatttaaa agatgaggta ttagaaggca cagtgacaag aataagccca
     1621 gatgtgcgag gtggtaacac taattattat gtaactttag attcaaataa agatataata
     1681 tttagggcta cttcaaaagt atctagcgaa ttgccattga ctcaggttgg tgataaagtc
     1741 aaaatatcgt attcaaaaga agaatctggt gtaatagaaa tgtcagaatt tgataaccta
     1801 aatattgcaa actttgaaga taaaaaagca aaagaaaaaa caggaactga tgaaaataca
     1861 gattccaaag attctgaaaa tcaaggtaat gtataattta aataaaaaga ttgataatag
     1921 tatttaacat agtgatagct aatttttata gaatttaatt ctataaaaat tagctatttt
     1981 attttaattc tttgtaaatt taatgtcata aaattaatat aatattagta caactttgca
     2041 aataatactt caaggagaat gtactatgac gaatatggaa aaagagatat tacttttaat
     2101 tagcagggtt agatgtgtta cagagtctca atttagaaaa atttatggag accaaaaaag
     2161 atacaataaa aaaaatttta aaaaaacttt gagaaaaatg tgtagtgaat atacactaaa
     2221 aaaatatcct tgtgatgtgg attattataa ttataaagac ctctcttatg tatactattt
     2281 aaacggtagt aaacttttta agggagatga tttaataaag gctctagtag gatcagaatt
     2341 agttgttaga atcaatactg caggttgtga tgtaaaaaga ttttaccgaa atgttagtgt
     2401 agatgatgtg aaatacgatt tatttattga atatacagac caatttggta atataaagca
     2461 agttttagtt gatgttgact taaataatga aatagacatt tctaaatacg aaaatctttc
     2521 taaaaaaata gataaatcta caattccatt ttttaaagta ccaaacgttt tggtagttac
     2581 accagataat ctatccgaat taaatgattt agagagatat gataatgagt atagcataaa
     2641 ttttatagat ttaggattaa ataaactatt taattgtata taagctaact ttagaaaaga
     2701 aaactatcga tttgccacat tttgtaaaat aattgttgca atattatata gaatatgata
     2761 aaactatatt aataaattaa ttaaaaataa tgagcctagg gtagaggtag ctgtattcaa
     2821 tagtattcaa taggagctgg taagcgttga taattgatga aagggtatat agccgaagaa
     2881 attttgttga cggacaaatt ttctggactt atgcagaata tgcataaggc tgtcactttt
     2941 aaagtggtga gctacaaggt tacttggtct atgaaattta cacgtccaaa gtataccttt
     3001 gggcgttttt atttatcaaa atttagcttg agggggagaa agcatgagta taattgttca
     3061 aaaatatggt ggaagttcag tagcagatac tgaaaaaata aaatcaatag cagaaaatat
     3121 aatagaacga agaaaagaaa atccacaaat ggtaatagtt gtgtctgcca tgggaaaatc
     3181 cactgatgaa tatataactt tagcaaagga attgagtaat gaacctagta agagggaatt
     3241 agatgcttta atgtctactg gtgaaatgat ttcagcatct ttattatcaa tagcattaaa
     3301 tgcacttgga tgtaaagcta taagttataa tgcttaccag ttaaatataa aaacgagtgg
     3361 tcttcatgga aagtctcaaa ttgatgatat aaacgtaaac agaattaaaa atagtttaga
     3421 tgaaggaaat gtcgtgatag taactggatt tcagggaatc aacgaagatg gtgatgtaac
     3481 cactcttgga agaggtggtt cagatacatc tgctgtagct cttgcggtta aattaaatgg
     3541 aaaatgtgag atatatacag atgttgatgg aatttatttt acagacccaa gaaaatattc
     3601 aaaggctagt aaacttgatg agattgaata tgaagaaatg cttgagttag cgagtttagg
     3661 agcacaagta atgcattcaa gaagtataga gcttgcacaa aaatatggtg ttgaaatcta
     3721 tgtcggacgt acatgtggta cagaaaaagg aacatatata agagggggaa aagacatgaa
     3781 attagaggat aaagtaataa caggattagc tactagtgat gatgacagtt ctataacaat
     3841 aaaagatttt aaagctgaaa atatatctag cttatttgaa gatatagcaa ctataggtat
     3901 aagtgtagat atgataagcc aaactgcacc tattttagat aagataagtg tatcatttac
     3961 agttccaaaa gaagagttgg gagaatgtaa aaagatagta tctaaatata cagatgaaga
     4021 acatgtagtg attgataata atatcactaa attttcttta gtaggtcttg gtatgaaaaa
     4081 tacttctgga gttgctgcta aagtatttaa gatattcaat gaaaatggta taatgataaa
     4141 acttataact acatcagaga ttcgtataac ttgtgctata aattctgatg ataagcaagt
     4201 agctatagaa aaaatagcag aagtatttaa tatataaaat ttggggaggc tattatgaaa
     4261 cttcatggaa ctatgactgt aaataatgat gaattatata ttggaggagt gagttgttta
     4321 gaacttagta aaaaatatca aactccactt tatgtatttg atgaagcttt agttagacaa
     4381 aattgtagag aatatgtaga atattttaag gctagagagg gaaaaaataa ggttgcttat
     4441 gcagggaaag cttttttacc tctttacatg tgtaatctta taaatgaaga acaaatgtgt
     4501 ttagatgtag tttcaggagg cgagttatat acagcttata aatcaaattt tcctatggaa
     4561 aaagttttct ttcatggaaa caataagact aaagaagaaa taaacatggg attagattta
     4621 ggtgtaggta aatttgtggt ggataatttt tatgaattag atttgcttga agcattatgt
     4681 gaagataaag gtaaaactca agagatatac tttagaataa ctcctggtat agaagctcat
     4741 acacatgatt atataaaaac tggacagata gattctaaat ttggatttgc tttagtaaat
     4801 ggagatttat ttagagcaat agaaaaatta agtgaatata aaaatattaa acttgtgggt
     4861 cttcacgctc atataggttc acaaatattt gatgtagaac catacataga tacagtggat
     4921 ataatgatga acttggtaaa agaaataaaa gaaaaattca acatacaact aacagaaatt
     4981 gacctaggtg gaggagttgg cgtttattat agcgaaggag ataaacctaa aactataaaa
     5041 gagttttgtg aaaccataat cactaaagca gaaacttcat gtaaagagct tggaatagaa
     5101 gtacctacat tggtaataga accaggaagg tcaatagttg caaatgcagg aagtacattg
     5161 tatacagtag gttctataaa agatataaaa gatgttaggg tttatgtaag tgtagatgga
     5221 ggtatgacag ataatgtaag accttcgttg taccaagcta gttatgagtg tagtattgtg
     5281 aataaaatta atcatgaggc tatgaatcat gtaacaatag ctggaaaatg ttgtgagagt
     5341 ggagatatat taattggaga tataaacata atggatatta aaagtggaga tatacttata
     5401 acaacatcaa ctggtgcata tggatattca atgtcttcaa attataataa aataccaaga
     5461 gcagcagtag tatcagtatt tgatggaaat gataagataa tttgtaagag acaaagctat
     5521 gaggacttat tagctttaga gatataaaat ggagaagtat ataataaaaa ttaaatatta
     5581 aagtttatta tgagcagatt tgctaggata tgtatctcta aattaatttt tggtttattg
     5641 tatcgttcgt agcatatctg cttatttttt gttttactaa caaatttctt gtaataaata
     5701 taaaatattt taaaaacttt ataattatga taaaatttaa agtatatgac aaggagggat
     5761 ttaatgagag gaagagtaat tttattaata tcgatattat ctatatttct tgtaggatgt
     5821 tcatttaata aaaaagatga aataaattta gtagagcaag gaaaaacata tttagaaaaa
     5881 cataactata aaaaagctat ggaatcatta tcctctgcac tggaagaaga tagtactaat
     5941 gagaatgcaa gagctatgta tatgcaagct atgagaatgt caaatatgac ggagtttgaa
     6001 gaattaaaaa attatgaggg tgcaatcaag gaacttaaac ttatagagaa aatcaaaaat
     6061 ggttcctctg ttattaaaga agaggcaact aaaaagaaag aagagttaac taaattagaa
     6121 aaagaacaaa acgaagcaga ggaacaaaga aagaaaaagg caaaagatac ggctggagaa
     6181 gataggtata gagttgaatc agatgcaagg aaatcttctt atagtggaag tagtaaaaat
     6241 aataaatcta gttctaaaaa taatgataaa aaatcagata gtaataagtc tgacaatagt
     6301 aacagttctt ctcaaaatca agaaaagcca aataatagta atccaacacc agcaccaact
     6361 ccacaagata ctactggtgg ttcagatagt ggtggaaatt ctcaaaactc aaataatcaa
     6421 taagtataaa atatatgcat aaatttaatg aacaaattat atattataaa aaatatctct
     6481 tagcaatttt ttcttactaa gagatatttt ttgagaaata atttaataaa taacagttaa
     6541 attttaaact aaaaattaaa gtcacagtag agataagcta ttgtaaacga ttatctatat
     6601 atttatcaac ataagttttt acaacagcag ctattggtac tgaaagaaaa actcctatga
     6661 aaccaaatag agattgtccc accaaaatag cagatattac ccatatagct cctataccaa
     6721 cttgattacc cataactttt ggttctataa aatttccatc taattgttgc aatataaata
     6781 gaaatgcaat gacccatata gttttttgtg gactatataa tatagtcatg gctattggag
     6841 ggattgcacc aataaaagga ccaaaatagg gaattatatt acctaagaat attattgaag
     6901 ctaaaaataa agcattttct attctgataa caaattgaaa tcctaaaaat gcaattatac
     6961 caataataag agagtctaat attctaccaa gtaaataatc atagaaaata tcatgagtag
     7021 tcctaaaaaa ttctataatg ttatctgcct tgtttctagc agtagaagaa tatagaaatc
     7081 tcttaaatcc caatgcaatt ttttctttgt catatataat gtatatagat agtataaatc
     7141 ccattgtcat attaaatagc atagaagata tactaagtgt atagattaag aagtttttag
     7201 gaattttacc aacaaaatta cttgcttctt ttaataaatt atctaaacta tgttgtatat
     7261 gaggaattaa agtttttaga aaatatatgt ttgacatatt tttttctata aaactatcta
     7321 tataatcagt atacataggc atttcattta tcaatctatt taatgtatta gctattgatg
     7381 gaattaataa cctaatggca aaggctaaaa taaaacctat aaatatataa gataaaaaaa
     7441 tggctaaaag tctatgtgct ttgaatttta tctcaaaaag catcacaagt ggatttaaaa
     7501 gtaaagcaag taaaatagct aataaaaatg ggctaaaaaa ctttaagaat ccttcaatac
     7561 tagatataaa gtctgatgga gtattaataa atttaaaaaa taaaattcca ataaacacga
     7621 ctattaaata atattttata ttttcctttg aaataataat cacttcctta aaatagtgtt
     7681 tagtcagcta ttacatatta atagtatgtg aaaagaaatt acaattattt aggaaaatta
     7741 aataattttt ttgcttgttt ttaattaagg tctagtatag tataataatg tagcaaaatt
     7801 aacattgata aaaatattaa acacacataa tttaagataa ggaaggatta agtatgagaa
     7861 ttaataagta tatagcttct tgtggaatag catcaagaag aaaagcagaa gaaataattc
     7921 ttggaggaag aataaaggta aatggaagtg tagtaaaaga gttatctttt aatattgatg
     7981 aagaaaaaga catagtagaa tttgataata aaaaagtaaa accaagtgaa aattatatat
     8041 atatagtatt aaataaacca gagggatata taactacagt aaaagaccaa ttcaatagac
     8101 ccagtgtaat agacatactt aaagatgtaa gggaaagagt atatccaata gggagattag
     8161 attatgaaac tagtggttta ttaatactta ctaatgatgg cgatttaaca tataagctta
     8221 cccatccaaa gcatgaaatt gataaaatgt atgtggcaag tgtgaaggga attataagtg
     8281 atgatgagat aagacaattc gaaacaggac taaaaataga agattacaca acggcacctg
     8341 ccaaaataag ggtaactaaa gaaaataaag aaaaaaatta ttcagtgtgt gaaataacta
     8401 tacatgaggg tagaaataga caagtaagaa aaatgtgcaa agctataaat catccagttt
     8461 taaatttaag aaggatttct gtggggaaaa tagtattaaa agacaccaaa gttggagaat
     8521 atagatacct aacagaagat gaaataaaat atttaaaatc aataaaataa gataaaaata
     8581 aattgagaaa aaatgaaata aagttaatag tagggagaaa tagtattatg gcatatgcaa
     8641 agtatttaac taaaattaca gatataaata aattttattt agaaaatatt ataaatgaag
     8701 gtgacatagt aattgatgct actatgggaa atgggtatga tacaaggtat ctagctgaga
     8761 aggttggaga aaagggcttt gtatatgctt ttgatataca agaagaagca ataaagtcta
     8821 caagaaagaa actagagaaa gaaggatata cagatagagt aaaattgata tttgatgggc
     8881 atgaaaaaat gaatcaatat ataaaagaag aagtttcatg tgttttattt aatttgggtt
     8941 atcttccaag agcgaaacat tcaattataa ccaaaccaga tacaacttta aaggctataa
     9001 agttaagttt agaaatgtta aaagaaaatg gagtaataag cattgccata tatacagggc
     9061 atgatggagg tcaagaagaa aaagattgca tatataacct tgtcaataat ttaaatcaag
     9121 atgaatttaa tgttttagaa tctaagtttt taaatcaagt caataaccca ccacaattga
     9181 ttttaattga aaagaaaaaa gcataataaa tactaaaaaa cttgacaaga ttgtattaag
     9241 agttaatata tatagtgtga cctattaaaa atactttaaa taataagtat acaacagttt
     9301 attctcaaaa caaaatatac atctaaatat gcaaaataag atagataact tttaaatgag
     9361 taaaaattga aaaatttaaa agaaatattg gataaatatt aaaaaatgca aggaaagatg
     9421 gtataataaa tttaatcgaa aatactatgt gaagtataag gtacagaaaa gttagaattt
     9481 tatgagttta agaagctatg ataaaaacta agatgaaatt gagataaaaa ataaaatttt
     9541 tagagaaaaa gacagtgtta ataaaacaaa atagtttata taactaaaag gagaaaaaaa
     9601 tggaagaaac aaatgaaatg aaggacagta aacatttgat atcaaatata caatctcaat
     9661 atacgagatt gagtaagggt caaaaactta tagcacaata cattttaaat aattatgata
     9721 aagttgcatt tatgactgca tgtaaattgg gtgaaactgt aggagtaagt gagtctacag
     9781 ttgtaagatt tgcaaatgca ttaggttata gtggatatcc taaattacaa gctgcacttc
     9841 aagaactcat aaaaaataaa ctaactacag tacaaagagt tgaaatggca catgattatt
     9901 ccgatgattt tgcaatacta aacaaagttt taaaaagtga tatagataat ataaggtcta
     9961 cactagaaga aattgacgag agagcgttta aagaagcttc taacaaactt ttaagagcga
    10021 gaaaaatata tatacttgga atgaggagtt cttttgtagt agctcaatat ttaggttttt
    10081 acttagatat aatacttgac aatgttcata taattagaat ggatatggga gatgcttttg
    10141 agcagattgt aagaataaat gaagaagatg taatagttgc tattagtttc cctcgttatt
    10201 ctaagaaatc ttatcaaatt gtcaattatg ctaaagaaaa aggagcccat gtaatatcat
    10261 taacagatag cttatttgcg ccagtagcat cacttgcaga taacactctt ctagttaaga
    10321 gtaatatggc ttcatttgta gattctctag ttccagctct tagtatttca aatgctcttg
    10381 ctatatctgt tgggatgaag gaaaaagaag atattaaaca gcattttgat gatttagagc
    10441 aaatttggaa aagatattct gtgtatgagt aaagtttgtg tattaataag gctatgaaat
    10501 tgagaatcat agccttatta ataaatacta gattatcact taaaaaataa attttcgttt
    10561 aaataataca aataaatatt tcgagactca aaacataaag atgtattctg aatatatctt
    10621 tactacatca actaatttat taaagaaagc atttcccttc atggagagtg ctttttttat
    10681 ttctctaaac aatatcattt ccttaattaa caattataaa attaatttta tttgtattta
    10741 agtatataat atcttattaa aattctatta aaatatgtat ttctatcttt tttgttcact
    10801 ataatatgtg tcttttagta ttttatttta actttgacaa agaagttgaa aaatttaaaa
    10861 atcatagaat tagtagttag gtatgaaatc ttgatttttt aagtagccat ttttggggaa
    10921 agggaaactt ttttagaaat a
//
LOCUS       DAEPIN010000099        10358 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.101, whole genome shotgun sequence.
ACCESSION   DAEPIN010000099 DAEPIN010000000
VERSION     DAEPIN010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10358
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.101"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            224..1003
                     /locus_tag="KR250_003308"
     CDS             224..1003
                     /locus_tag="KR250_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HBH0069199.1"
                     /translation="MSYKKIAIITGANSGFGKEFLKLLINEEEITEIWAVARNKARLN
                     QLVDEFGSKVKIFSKDLSKIEEVKEIGTFLSKENVCIKYLINNAGFAKFCSYNDLSID
                     ESINMIDLNISAVVALGLICIPYMQKGSHIMNISSQASFQPLPYQNIYSSTKAFIRNY
                     TRALNVELKSKGINAIAVCPGWMSTNLFKRGIVVAEKGTKNFPGIVSPDIVAKKALKD
                     AKKNKDISVYGINTKSSHLLAKLLPQKMMMKVWLMQQHIKE"
     gene            complement(1313..2134)
                     /locus_tag="KR250_003309"
     CDS             complement(1313..2134)
                     /locus_tag="KR250_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002572585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallohydrolase"
                     /protein_id="HBH0069200.1"
                     /translation="MKLKIVGSGGMSIIPSSFCKCKICEEARQKGGRYERLGPSLYID
                     DIKMLIDTPEDIAVACNRQKISEVKHISISHHDPDHVKGIRIVEKIGCDFITGENNPI
                     GFYALPEVIEDINRTNLDCLKYYGNVINCISINETSHVKINNFDIDLINNNPDRNITF
                     YVIKENEKKVIYACCNPKPFTHSDMYFDADVLIISLVSDDGILNDGTKLKDTPFKDEI
                     FTLDEIVEIKNYYRIKKIIITHIDEIWGKSYGYYSELEKKLDNIYFAYDGMEIIV"
     gene            complement(2809..3627)
                     /locus_tag="KR250_003310"
     CDS             complement(2809..3627)
                     /locus_tag="KR250_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBH0069201.1"
                     /translation="MFKIGDFSKLSKISIRMLRHYDEIGLLTPSHTNKTNGYRYYSAD
                     QLSTTNRIHALKDMGFGLYSIKEILTEYNDKESLIKYLNIHHSQVKEQLEDTQKKLLK
                     IETTIKRIGGNDIMKNYDVTIKNFAPKYMMTLRRVIPTYQDEGMLWHQVFLETKDQNV
                     QIEPPKYSKAVFYDTGYKEDYVDVEVQVAVSGKYKDTEHVKFKTVPSVTAATAIVNGN
                     FNQVADACEAIGNWISDNNYDVDGPMFNIYHVSPGNDSNPDNWVTEVCFPVKKK"
     gene            3795..4376
                     /locus_tag="KR250_003311"
     CDS             3795..4376
                     /locus_tag="KR250_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBH0069202.1"
                     /translation="MKSNDKDARERIIEVTLNLLNEVDDIEEITVRKIAERANVGVGL
                     INYHFKTKDNLLSTAIGDVMSNIIAELYDDSVYTLRPIEDLKNLLKKLCDTGLHYEKV
                     LPFVLNQCITNGDMQAELDIVPMLRKIFGNKKDEMSLRIIALQIILPIQISALSTESF
                     QLYSGINIKNKYERDKFIDILIENIIGEDVDVR"
     gene            4373..4567
                     /locus_tag="KR250_003312"
     CDS             4373..4567
                     /locus_tag="KR250_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069203.1"
                     /translation="MKIITIGSSLITVLLFLSTMVCGFWIKNNKVTDASSIKFHMNSA
                     IFTGIFLLISTILLIIYIKK"
     gene            complement(4731..6473)
                     /locus_tag="KR250_003313"
     CDS             complement(4731..6473)
                     /locus_tag="KR250_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M3"
                     /protein_id="HBH0069204.1"
                     /translation="MVKSKKKIFVFLILIAVFIIFASYIRNYTNDTKKDSVRGTIPFE
                     KMEYKRPNIQSICENINKCNDKLLTAKTAKEQLNLFNEVDKIYQDFYSTLTIAKIRNN
                     IDSSDEFYSKEYKYLMSKSVDVDMSYKDFQDRFVTSKFSKELEKELGKDTFNYLKNIG
                     KLNSKEVEGLLKKEQDLVVKYEDLLAKSTVSIDGIEVDFEEALSRPNLSPEEYVKIYS
                     DYLKKYNPIFGNIFLELIQTRTEIASKQGFKNYIDYAYMNLNKDYSQEEAKKFRQDVK
                     DYIVPLYREISSKPSDSSIYIKVYKNRSFRKFDKVLEDISPKLKESFDYMKKYDLYDY
                     SSGKNKSPGGYTTYINKYKAPFLFNTWDNSFLGVTSFAHEFGHFYNYYNSINLNNKMQ
                     PSIDVCEVHSTSLEILFYKYFDDFFGKQSEAIKKEHLSIVLNTIIDACLYDEFQEIIY
                     KNPYMSLNDINKLFFDLEEEYGVSNNILREKNAPFWILVSHNFQVPFYYLSYGLASDV
                     SLQIWQLSQEDYRKAVDVYMDFLNQNTDAGFKDVVEKVNLQLPFQNGNLEEISSTLYD
                     YFGIDNPLELKNAS"
     gene            6902..8278
                     /locus_tag="KR250_003314"
     CDS             6902..8278
                     /locus_tag="KR250_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HBH0069205.1"
                     /translation="MKTIVRRLILYADGMRPTQIMVSGFAAIIVIGALLLTLPIASQS
                     GESIGLLNALFTATSAVCVTGLVMVDTATYWSLFGQIVIITLIQIGGLGFMTVATMFS
                     LMARKKIQLRERLLIQESLNQADLSGLVRLTRFVLIITITIEGIGALVLSTVFIPQFG
                     LSKGIWYSVFHAISAFCNAGFDLMGSVSGPFTSLNSYVNNFTVSMTVCALIVLGGLGF
                     PVVLDIVRKRRFSKLNVHSKVVLFSTATLIFVGALFIFLIEFNQKATMADLPLKGKVL
                     SAIFQSVTARTAGFNTLDLATLRESSVFVMIILMFIGASPASTGGGIKTTTLAVLIIT
                     VRSFLSGKSDIEAFERRLAPSTIKKSLGIFVISISAVIFGTLIISITQPNFTLVQSAF
                     EVTSALATVGSSLAGTPNLNALGKIIIIIFMFMGRVGSLTLFMAILSGGRRKSQPIRY
                     AEGKIMVG"
     gene            8565..9236
                     /locus_tag="KR250_003315"
     CDS             8565..9236
                     /locus_tag="KR250_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HBH0069206.1"
                     /translation="MKQYIVIGCGRFGSSVASTMHLLGHQVMAIDKNEDSVQSISDKV
                     THSLIVDVTDEQALRSLGLGNFDVAVVAIGSDIRASIMATLIAKEMGVELIICKAKDE
                     LQAKVLYKIGADRVVFPERDMGVRVAHNLVSDNILDHIELDPEYSIVEIVTPNSWVGK
                     TLIELELRARYEITVLAIKTGKNINVTPSPDEELTAGSILVIIGQNTSITAITSGNKG
                     IIRRR"
     gene            9425..10222
                     /locus_tag="KR250_003316"
     CDS             9425..10222
                     /locus_tag="KR250_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HBH0069207.1"
                     /translation="MITNINSKDNEKLKYTRALLKSKNRNKESKFIIEGYRIVMLALE
                     CMANLDYVFINEEFENKKEHVKLLEDLDKKNTKIYKTTNKNFKELVDTENTQGIIGVV
                     SFKKKKLSESINKKDKFVLILDRIQDPGNMGTIIRTADSAGVDAIIALKGCVDIYNPK
                     VIRSTMGSIFDVNIIDASQDETVDMLKSLDFNIVSSYLNTENFYDKIDYGSKVALVIG
                     NEANGINEELVSKSDILVKIPIYGKAESLNAAISSAILMYEIKKYLI"
BASE COUNT         3834 a         1251 c         1572 g         3701 t
ORIGIN      
        1 atcaataccg attctataat ataattaaca ctacttttta aatataatca ataccgattc
       61 tataatatta acactatttt ttatgctata taacaattat tgaaaatgat ataaatataa
      121 tatctcaaat aatatgaata taacatctga atagagggtg ttttatttgt aaaaaataag
      181 tttacagttt gagcgtataa acttatggta aaggagaata attatgagtt ataagaagat
      241 tgcaattata acgggtgcta atagtggttt tggtaaagag tttctaaagt tattaataaa
      301 tgaagaagaa ataacagaaa tatgggcagt tgcaagaaac aaggcgagat taaatcaatt
      361 agtagatgaa tttggaagta aggtaaaaat tttttcaaaa gatttatcaa aaatagaaga
      421 agtaaaagaa attggtacat ttttaagtaa agaaaatgta tgtataaaat accttataaa
      481 caatgctggt tttgcaaaat tctgttcata caatgattta agtatagatg aatctattaa
      541 tatgattgat ttaaatatta gtgctgttgt agcattggga cttatatgta ttccatatat
      601 gcaaaaggga agccatatca tgaatatttc ttcacaagca tcattccagc cattgccata
      661 tcaaaatatt tatagttcaa ctaaagcgtt tataagaaat tatacaagag cattaaatgt
      721 tgaacttaag agtaaaggga taaatgcaat tgctgtatgt ccaggttgga tgagtacaaa
      781 tctatttaag agaggtatag ttgtagctga aaaagggaca aaaaatttcc caggcatagt
      841 gtctccagac attgttgcca aaaaagcatt gaaggatgca aagaaaaata aggatatttc
      901 tgtgtatggt ataaatacaa aatccagtca tttattagca aaactattgc cacaaaaaat
      961 gatgatgaag gtttggttaa tgcaacaaca tattaaggaa taagagtttc ggtaattaag
     1021 tatcagttgg agattcaatc tgttatatat tatgagaagt tttgttgagc aaataataat
     1081 tgtgacaaaa cggcttatct agtgccgata agccgtctat aatgtatata aaatatattt
     1141 ttgattttac tttatttaga tattgaagta ttaaaatatg taggacttga aattttataa
     1201 agataaatac tatgatattt tgtaaaaact gagatatgtt gatattacat agtttaacta
     1261 tactgttaaa ttgaagtttt cttattttgc attatgctta ttacttaatg atttagacta
     1321 taatttccat accatcatat gcaaaataaa tgttatcaag tttcttttct aattcactgt
     1381 aataaccata tgattttccc catatttcgt caatatgtgt aatgataatc tttttaattc
     1441 tataatagtt tttaatttca acaatctcat ctaaagtaaa tatttcatct ttaaatggtg
     1501 tgtctttaag ttttgtacca tcatttaata taccatcatc agaaactaga cttattatta
     1561 aaacatcagc atcaaagtac atatcactat gagtaaatgg cttgggatta cagcaggcat
     1621 atatgacttt tttttcattc tctttgataa catagaaggt tatattcctg tctgggttat
     1681 tgttaatcaa atcaatatca aaattattta tctttacatg cgaagtttca ttaatagaaa
     1741 tgcaatttat aacattacca taatatttaa gacaatcgag attcgttcgg tttatatctt
     1801 caattacttc aggaagcgca taaaagccaa ttggattatt ttctcctgtg ataaagtcac
     1861 atcctatttt ttcgacaatt ctaataccct taacatggtc tgggtcatgg tgagaaattg
     1921 aaatatgttt tacttccgat attttttgtc tgttacaagc tactgcaata tcttcaggtg
     1981 tgtctatcag catttttata tcatcaatat ataaacttgg ccctaatcgt tcatatcttc
     2041 cgcctttttg tctggcttct tcacatattt tgcatttgca aaatgaactt ggaataattg
     2101 acataccgcc actaccaaca atttttaatt tcaaagttat tacaccacct tcttaaaata
     2161 ttagattata taaataatct acaatcacaa ctgattaaca aaattatgag ttaataaatt
     2221 atatcacatg taaatatgaa agaatagtat gaaattttta taaacatata ttaaatgttt
     2281 ctcaatgaaa ttgactttgt atatgaactg aatgacttat tcaatatgga taagacattt
     2341 ttcttgtaat aagatatgat tgtcctataa ggtttatgta attttgatat aatttgatat
     2401 aatatattga ttaacaacat acatgttatt ggtgagcata tttctataga atggaatata
     2461 taatttttta tatttatcat tgtattattg tctaaagaaa gttaaaaaga gtagagcaca
     2521 aaggaattat ttgtacttta ctcttttatt gttttgttaa aaaaactaat ttatattagt
     2581 gattaaaata tatttaacta gagtataagg atagtatata atcttataat tgatttatct
     2641 gacaaattaa gataatatcc taattaaatt gtagatattt atacataaca ctaaaagtaa
     2701 tttgtttaac aaattaagat aatatcctaa tcaaattgta ggtatttata cataacacta
     2761 aaagtaattt gtctaacaaa ttaaaatatt atccaattaa tataaaaatt acttcttttt
     2821 aactgggaag catacctctg ttacccaatt atcaggatta ctatcgttgc cagggcttac
     2881 atgataaata ttaaacatag gaccatccac atcatagtta ttatctgata tccaattacc
     2941 aatagcctca caagcgtctg caacttgatt gaaatttcca ttaacaattg cagttgcagc
     3001 agtcacactt ggaacagtct taaatttaac atgttcagta tctttatact ttccagatac
     3061 agcaacttgt acttcaacat ctacataatc ttctttatat ccagtatcat aaaaaactgc
     3121 tttagaatac tttgggggtt caatttgaac attctggtct tttgtctcta aaaatacttg
     3181 atgccataac attccttcgt cttggtaagt tggtataacc cttctaagtg tcatcatata
     3241 ttttggtgca aaattcttta tagttacatc ataatttttc ataatatcat ttcctccaat
     3301 tcttttaata gtggtttcta tttttaatag ttttttctgt gtatcttcta attgttcttt
     3361 tacttgagaa tggtggatat ttagatattt tatgagactt tctttatcgt tatattctgt
     3421 caaaatttct tttatagaat acaagccaaa gcccatatct tttagtgcat ggattctgtt
     3481 tgtagtagaa agttggtctg cactatagta tctatatcca ttagttttat tcgtatgaga
     3541 aggagttaaa agtcctattt catcataatg acgtaacata cgaatactaa ttttacttag
     3601 ctttgaaaaa tcgcctatct taaacatgtt tttgtctcct ttataaaggt acttttagct
     3661 taatattaat ataaaccata tcataatgtt agagtcaact aaaattataa ttaaattata
     3721 ttgacatatg atgaaaaaaa tattatacta aacatgttca gaaccgaaaa tgttaattgt
     3781 tttggaggtc tattatgaaa agtaatgata aagatgctag agaacgtata attgaagtta
     3841 cattgaatct tttaaatgaa gtagatgata ttgaggagat tacagttaga aaaattgctg
     3901 agcgtgcaaa tgttggagtt ggtttaataa attatcattt taaaacaaag gataatttgt
     3961 tgagtactgc cataggagat gtgatgtcaa atataatagc agagctatat gacgatagtg
     4021 tatatactct aagacctatt gaagatttaa aaaatttatt aaaaaaattg tgtgatactg
     4081 gattacatta tgaaaaagtg cttccttttg tattgaatca atgtataacc aatggagata
     4141 tgcaagcaga acttgatatt gtccctatgc ttcgtaaaat ttttggaaat aaaaaagatg
     4201 aaatgagtct aagaatcatt gccctgcaaa ttattttacc aatacagatt tctgctttat
     4261 ctacagaatc atttcagtta tacagtggta taaatataaa gaataaatat gagcgagata
     4321 aattcattga tattttaata gaaaatatta ttggagagga tgttgatgta agatgaaaat
     4381 tataactatt ggttcaagtc ttatcactgt tttacttttt ctttcaacta tggtttgtgg
     4441 gttctggatt aagaataata aggtaacaga tgcaagttct attaagttcc atatgaatag
     4501 tgctattttt acagggatat ttttattgat ttccacaatc cttttaatta tatatataaa
     4561 aaaataataa tattattaat aaataaagta tcagaaatta taaagacaag agtcttaaaa
     4621 atttctgata ctttaccata taattttatt aaacattaat tgtgtgtgaa aaaatatcac
     4681 ttatttcaat ctaatactaa atcttaagag tgagtataaa gtattagaaa ttaagaagca
     4741 ttttttagtt caagtggatt atctatacca aaataatcat ataaagtaga gcttatttcc
     4801 tctaaatttc cattttggaa aggtaattgt aaatttacct tttctaccac atctttaaat
     4861 ccagcatcag tattttgatt taaaaaatcc atatatacat caacagcttt tctgtaatct
     4921 tcttgtgata attgccatat ctgaagtgat acatcagatg caagaccata actcaagtaa
     4981 taaaaaggaa cttggaagtt atgagataca agtatccaaa aaggagcatt tttttctcta
     5041 agtatattat tggaaactcc atattcttcc tctaaatcaa aaaacaattt attgatatca
     5101 tttagactca tatatggatt cttgtatata atttcttgaa attcatcata aagacaagca
     5161 tcaataatag tatttaaaac aatcgataag tgttcttttt ttatagcttc agattgttta
     5221 ccaaaaaaat catcaaaata tttataaaat agaatttcta aagatgttga atgaacttca
     5281 catacatcta tacttggttg catcttatta tttaagttaa tactgttgta gtaattataa
     5341 aaatgaccaa actcatgagc aaaactagta acacctaaaa aagaattgtc ccatgtatta
     5401 aacaagaaag gtgctttata tttatttata taagtagtat atcctccagg tgatttgttt
     5461 tttccagaag aataatcgta caagtcatat tttttcatat agtcgaagct ctcttttagt
     5521 tttggagata tatcttcaag tactttatca aacttcctaa atgagcgatt tttatatact
     5581 ttaatataaa tagaagaatc agatggttta gatgaaatct ctctatataa tggaactatg
     5641 taatctttta catcttgtct aaatttttta gcttcttctt gagaatagtc tttgtttagg
     5701 ttcatatagg cataatctat ataattctta aatccttgct tacttgctat ttcagttctt
     5761 gtctgtataa gttctaagaa tatgttacca aatataggat tgtatttttt taagtaatct
     5821 gaataaattt tcacatattc ttcagggcta agatttggtc ttgacaaggc ttcttcaaag
     5881 tctacctcta taccatctat tgatactgta gatttagcta ataaatcttc atattttaca
     5941 actaggtctt gctctttttt aagcaaacct tcaacttctt tagaattaag ttttccaatg
     6001 ttctttagat aattaaatgt atcctttcca agttcttttt ctaattcttt agagaattta
     6061 gaagttacaa atctatcttg aaagtctttg tatgacatgt caacatctac tgatttactc
     6121 ataagatact tatattcctt agagtaaaat tcgtctgatg aatctatgtt atttctaatt
     6181 tttgctatag taagtgtact gtaaaaatcc tgataaattt tatcaacttc attaaataaa
     6241 tttaattgtt ccttagctgt tttagctgtt aaaagtttgt cgttacactt gttaatgttt
     6301 tcgcaaattg actgtatatt aggacgctta tattccattt tttcaaaagg aatagttcct
     6361 ctaacagaat ctttttttgt atcattagta taatttctta tgtatgatgc aaatataata
     6421 aaaactgcaa ttaaaattag aaaaacaaat attttcttct tggatttaac cataaaaatc
     6481 ctccaatcat ggatatagtt attaattatt ttactatatt aaatataaaa aatgcatgtt
     6541 aagaaaattt aacataaaga tatagataag attataaata atataaaaaa tttaacttca
     6601 aaaaagtata tgaaatttat actggtaaaa tgataaataa attttaagta caaaatagta
     6661 aaagctatga tagttttaga cctattatat gtaaaaaata tagatgtttt tggtgcaatt
     6721 taaattaaaa gatatatata aaaatttaaa atattccaaa cagaaattat aaaaattact
     6781 atcattgtct ttatacaata tttatactat aatagaataa taatttagag gaaagctcta
     6841 aaaataaaca aattaagggt cttaacagta gtgattttat gttaaggagg tataaagaaa
     6901 gttgaaaaca atagttagaa gacttattct ctacgcagat ggaatgcgtc ctactcaaat
     6961 aatggtatct gggtttgctg cgattattgt aatcggagca ttgttgttaa cactcccgat
     7021 agcgtcgcag agtggagaga gtataggctt attaaatgct ctatttacgg ctacttcagc
     7081 ggtttgtgtt actggtctag ttatggtaga tacagcaact tattggagtt tatttggaca
     7141 gatagttata ataacattga tacaaattgg tggtcttgga tttatgaccg tagccacaat
     7201 gttttcactt atggcacgaa aaaaaataca actaagagaa agattattaa tacaagagtc
     7261 attaaatcaa gctgatttat cgggacttgt aagacttaca agatttgtat taataataac
     7321 cataacaata gagggaatag gtgcactggt tttatcaact gtctttattc cacaatttgg
     7381 tttgtctaaa gggatatggt atagtgtatt tcacgcaata tcagcgtttt gtaatgccgg
     7441 atttgacttg atgggcagtg taagtggccc atttacatcg ttaaattctt atgtaaacaa
     7501 ctttacagta tcaatgactg tttgcgcact tatagtgctt ggagggctag gattccctgt
     7561 tgtacttgat attgtaagaa aaagaagatt ttcaaaatta aatgtgcatt ctaaggttgt
     7621 attgttttca actgctacac ttatttttgt gggtgcatta tttatttttc ttatagaatt
     7681 taatcaaaaa gctactatgg cggacttgcc tttaaaggga aaagtactat cagcaatatt
     7741 tcaatcagta acagcaagaa ctgctggttt taatacgctt gatttagcta ctttacgaga
     7801 aagtagtgta tttgtaatga taattttgat gtttattgga gcatcacctg catcaactgg
     7861 tgggggtata aaaacaacaa cactagctgt tttaatcatt acagttagaa gttttctatc
     7921 aggaaaatca gatatagaag cttttgaaag aagattagct ccatcaacta taaaaaaatc
     7981 tcttggtata tttgtaatca gtatatcagc agttattttt ggaacattga ttatttcaat
     8041 aactcagcct aattttacat tagttcaatc agcttttgag gttacatcag cacttgctac
     8101 agttggttca agtttagctg gaactccaaa cttaaatgct ttaggtaaga ttataataat
     8161 aatctttatg tttatgggta gagttggttc tcttacttta tttatggcta tactttctgg
     8221 tggtagaaga aagagtcaac caatcagata tgcagaaggt aaaattatgg ttggttaagt
     8281 taccaaggag taatataaat ttataatgga tttagagtag cactgtaaat ctgttgataa
     8341 aataaatgag cctagatgta attatatgaa gaaataaaat cgactaacga acaaaattaa
     8401 ttaatgaaat aaaaaaacag ggaaataaaa tacaaataaa aaggtaaatc aaaattaaat
     8461 taatctgtgt atataaaatt atatattgtg tataagtttt gtatgtgtaa acaattaatt
     8521 taagagatat tatataaatt gtgtattagg gaggaagttt cgagatgaag caatatatag
     8581 tcattgggtg tgggagattt ggaagttcag ttgcgtctac tatgcatctt ttaggacatc
     8641 aagtaatggc aatagacaaa aatgaagatt cagttcaaag tatatctgac aaggtaaccc
     8701 attcacttat agtggatgtt actgatgagc aagcgttaag gtcattaggt ttaggtaact
     8761 ttgatgtagc agtagttgca ataggttctg atataagggc atctataatg gcgactctta
     8821 tagccaaaga aatgggtgta gagttgataa tatgtaaggc aaaggatgaa ttacaagcta
     8881 aagtgcttta taaaattggc gcagatagag ttgtatttcc agaaagagat atgggagtaa
     8941 gagttgcaca caatttagtt tcagataata tattagacca tattgaactt gacccagagt
     9001 attcaattgt tgaaatcgta actccaaata gttgggttgg caagacactt atagagcttg
     9061 aattaagagc tagatatgag ataactgtac ttgctataaa aacaggtaaa aatataaatg
     9121 ttacaccttc tccagatgag gaacttacag ccggaagtat cctagttata atcggtcaaa
     9181 atactagtat aacagcgata acatctggaa ataaggggat aattagaaga agataattta
     9241 ctatttaata tatatttaat tgcaatgaaa gtagatagta tcatataatt atgaatagtt
     9301 atatgatact atttttttaa tcgaaggtag tatttatttg taagattaga taaagagaaa
     9361 ttaaattaaa agtaaggagg ctgtgctaat ataaaaattt ataagttatt agcatttaga
     9421 taaaatgatt acaaatataa acagtaagga taatgaaaag ttaaagtata caagagcact
     9481 attaaaatca aagaatagga ataaagagtc aaagttcata atagagggat acagaatagt
     9541 aatgcttgca cttgaatgta tggcaaacct tgattatgta tttatcaatg aagaatttga
     9601 aaataagaaa gaacatgtaa aactattaga agatttggat aaaaaaaaca caaagatata
     9661 caagactact aataaaaact ttaaagaatt agtggataca gaaaatactc aaggaataat
     9721 aggtgtagtt tcatttaaga aaaaaaaatt aagtgaaagt ataaataaaa aagataaatt
     9781 tgtattgatt ttagacagaa tacaagaccc aggaaatatg gggactataa taaggactgc
     9841 tgattctgct ggagtagatg ccataatagc actaaaggga tgtgtcgata tatacaaccc
     9901 aaaagtaatt aggtctacta tgggttctat ttttgatgtg aatataattg atgcttcaca
     9961 agatgaaact gtggacatgc ttaaatcatt ggattttaat atagtttcaa gttacttaaa
    10021 tacagaaaat ttttatgaca aaatagatta tggctcaaaa gtagcattgg tgataggaaa
    10081 cgaagcaaat ggaataaatg aagaacttgt atcaaagtct gatattttgg ttaagatacc
    10141 tatatatggt aaagccgagt cgttaaatgc tgcgataagc tctgctatac tgatgtatga
    10201 aataaaaaaa tacttaattt aatgtattgt aataaatata atgtcatgtt ataatcttga
    10261 attaaatagt agagtatcaa aaatagtcaa tatatattaa aaaaataatt aaattaatta
    10321 tatattagat gtatattata taaaaaataa ttgagtta
//
LOCUS       DAEPIN010000100        10328 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.102, whole genome shotgun sequence.
ACCESSION   DAEPIN010000100 DAEPIN010000000
VERSION     DAEPIN010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10328
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.102"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            complement(46..207)
                     /locus_tag="KR250_003317"
     CDS             complement(46..207)
                     /locus_tag="KR250_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069208.1"
                     /translation="MNNFLLNVIAGVIASLIFCLICKVFLKVKSHSTRGKSKSGWEFD
                     FKIKFHKFK"
     gene            complement(548..988)
                     /locus_tag="KR250_003318"
     CDS             complement(548..988)
                     /locus_tag="KR250_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBH0069209.1"
                     /translation="MSNLSAFLSQNAIKVDNVKYVASNRFLDKEGKPVEWELKVLSSE
                     EDEALRRKCTKRVKVIGNNGKHTGQYTSEIDYNSYVAELCVASTVFPDLKDAELQNSY
                     GVMGEAQLLKTMLTAGEYVNYTVKVNEVNGFDTSFEDKVEEAKN"
     gene            complement(1060..1530)
                     /locus_tag="KR250_003319"
     CDS             complement(1060..1530)
                     /locus_tag="KR250_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HBH0069210.1"
                     /translation="MAQTINAKDTVSAKKAECFITIEGKRYNFMQAIDLEAKMEKNKS
                     EVPILGRTTKGNKTTGSTNTGSATFHYNTSIFRELLYRYKETGEDIYFDIQVTNEDPT
                     SAVGRQTVVLKDCNMDSGIITKFDADGEYLDEDMDFTFEDWELVEKFNLLAGME"
     gene            complement(1547..2857)
                     /locus_tag="KR250_003320"
     CDS             complement(1547..2857)
                     /locus_tag="KR250_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath family protein"
                     /protein_id="HBH0069211.1"
                     /translation="MALGGGTFVTQNKILPGSYINFISAKRATSSLSDRGIVAIPLEL
                     DWGIDEDVFQVTSDDFEKYSVKYFGYDYTHEKLKGLRDLFKNIRLGYFYKLNKGVKAS
                     CTIATAKYSGIRGNDLKVTVTTNIDDNAKFDVVTLLDNKKVDTQIAKVITDLQDNDYI
                     TWKKDATLEASAGLVFTGGTNGEAVTGAEYQAFLDKIESYSFNALGCLATTTEIKSLF
                     VEFTKRMRDKVGAKFQTVLYKKSDADYEGVVSVENKIKDIGLVESSLIYWAAGVIAGC
                     DINKSNTNKKYDGEFDVDVNYTQIQLEEALKTGKFIFHKVGDEVHVLEDINTFVSFTD
                     DKNDDFSSNQSVRVLDQIANDIATLFNEKYLGKVPNDKAGRISFWNDVVKHHKELENI
                     RAIEDFKTDDVSVELGNDKKTVIVSDAVKVINAMSKLYMTVSVN"
     gene            complement(2859..3035)
                     /locus_tag="KR250_003321"
     CDS             complement(2859..3035)
                     /locus_tag="KR250_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069212.1"
                     /translation="MSKTLSKGTDYKFTKEQIVNSKKYINRKDLLNAILKENELYSFS
                     EVEEIINSFMKGVS"
     gene            complement(3028..3465)
                     /locus_tag="KR250_003322"
     CDS             complement(3028..3465)
                     /locus_tag="KR250_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069213.1"
                     /translation="MLNNIIDGISIKLDKTFGESYTIYSEDVEQGINEPCFFIVPLNP
                     SKVSYPSGRTLKKNSFDVHYFPKSNDKSFEIDEVAEMLLEELEYIEIDGDLVRGTNMN
                     FEIVDNVLHFFVDYNYFTIKNNDINKMDTVELFGGLKRGDNFE"
     gene            complement(3814..4620)
                     /locus_tag="KR250_003323"
     CDS             complement(3814..4620)
                     /locus_tag="KR250_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069214.1"
                     /translation="MAKFLDITGKKFGRLRVIKFSKEIKSGKRNRKYWLCKCDCGNFK
                     EIRTDSLTSGLVQSCGCLKKEQDKLNLTDKYQFKKKYKVQNKRLYSIWKGIISRCTDK
                     NNKRYNRYGERNIIVCDEWFCYDNFANWALSNGYSEKLTIDRINNEGNYESSNCRWVD
                     IKTQCRNRSTNILVKHEEKEITLIELSEKTGISYSCLRSRYSKGLVGNNLIEKVKIIE
                     ESRAKLSIEDVKEIRKKYSDGYTIKQLSEIYPVTYSSISNIVHRRTWKNI"
     gene            complement(4730..5020)
                     /locus_tag="KR250_003324"
     CDS             complement(4730..5020)
                     /locus_tag="KR250_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HBH0069215.1"
                     /translation="MARWGSVDFREFKRVCKKMEELTKIDLDKFCKDAARELAARLLG
                     KVIRRTPVDTGFLRQGWNGVAYARSLPVYKQGNNYIIEVVNPTEYALTQWGI"
     gene            complement(5020..5367)
                     /locus_tag="KR250_003325"
     CDS             complement(5020..5367)
                     /locus_tag="KR250_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069216.1"
                     /translation="MVSKTRKAIEMLYRDKCTIVEYQPIKDPVTKRTNNKEVIVLENQ
                     PCKLSYKNIVSATEGKVAKLEQTIKLFISPDIEIKAGSKLIINDKEYVRSGESAIYPN
                     HQEIILELFKDKA"
     gene            complement(5361..5762)
                     /locus_tag="KR250_003326"
     CDS             complement(5361..5762)
                     /locus_tag="KR250_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069217.1"
                     /translation="MENNIIDEIEKRLESFGYILKDGDKWLIGFVREKIENIIKLDCN
                     IKTMPIELKEIEVDMIVGEFLFTKKNMGQLDIESINFEAVEKSISEGDTKVDFAIGSG
                     SQTPEQRFDSLIAYLTTYGKNKILTFRCLRW"
     gene            complement(5772..6005)
                     /locus_tag="KR250_003327"
     CDS             complement(5772..6005)
                     /locus_tag="KR250_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069218.1"
                     /translation="MPQVKKLNRILTIEECKIDDFLEMGYDLIDETGKAVKYGKSLSV
                     KDLIAENNILRSKVESLEEENKQLKEKNKLTKK"
     gene            complement(6024..6914)
                     /locus_tag="KR250_003328"
     CDS             complement(6024..6914)
                     /locus_tag="KR250_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
                     /protein_id="HBH0069219.1"
                     /translation="MAALNYAKEYSNVLAQAYPYTLNFGDLYATPNNGRYRWTGSKTI
                     EIPTISTTGRVDSNRDTIAVAQRNYDNAWEPKVLTNQRKWSTLVHPADINQTNYVASI
                     GNITKVYNEEQKFPEMDAYCISKIYADWTALGNTADTTVLTTANVLEVFDKLMEKMTE
                     ARVPENGRILYVTPVVNTLIKNAKEIQRTVNIKDAGTSLNRQTTDIDTVKIIKVPSNL
                     MKTAYDFTTGWKVGAGAKQIFMSLVHPSAIITPVSYQFSKLDEPTAVTEGKFFYFEES
                     FEDVFILNKKADAIQFVVEA"
     gene            complement(6926..7489)
                     /locus_tag="KR250_003329"
     CDS             complement(6926..7489)
                     /locus_tag="KR250_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage scaffolding protein"
                     /protein_id="HBH0069220.1"
                     /translation="MDWLKELLEGIKIENNKIDVVSLQKSIEKKIKETTITQEDYTNL
                     ETQLNTANETIKKFEGGMTKEDVENLKTTYETDKKTLEETYKKEIEEKDFNYWLGDAF
                     KSVKCRDEIALKAHLDIEALRNSKDRQKAFEEQINPLKQDKDYLFNATLEGEEPKIDT
                     ITPGQEPKINDFGFNFTGVRPHENNNK"
     gene            complement(7534..7728)
                     /locus_tag="KR250_003330"
     CDS             complement(7534..7728)
                     /locus_tag="KR250_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015981492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069221.1"
                     /translation="MYILTQVIAVVCVVQIFINCIANVNVGILCNKLKEKNEANIDKV
                     SDEILKRVGEELNESLDKSL"
     gene            complement(7799..8041)
                     /locus_tag="KR250_003331"
     CDS             complement(7799..8041)
                     /locus_tag="KR250_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069222.1"
                     /translation="MIVLMNLLEKNNEYVIYSYGYEENKLDGRIKIYLDDFYNYEIIK
                     ESKDEHISKSATLKAISKLIKAAKNNDLKKEMSYQC"
     gene            complement(8007..9500)
                     /locus_tag="KR250_003332"
     CDS             complement(8007..9500)
                     /locus_tag="KR250_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HBH0069223.1"
                     /translation="MKHKDYWRKRFEQLEEAQNNKSVKYYLELEKQYKLAMNSIEKDI
                     LAWYNRFAKNEGISLLEAKKLLNTRELEEFKWSVEEYIKYGKENAINQKWMKELENAS
                     ARVHITRLEALKLQIQQQVEVLYGNELDGIDKLMRDIYTSGYYHTAFNVQQGVNVGWS
                     LMSLDTNRINKIISKPWAIDGLNFSERIWGKYRPTLVNELHTKLTQSIIRGENPKNLV
                     NDFAKRFNVSKSQAKNLIMTESAFFASASRKDCFSDLEVEKYEIIATLDLRTSNICRE
                     LDGKIFDMKDYQVGITAPPFHCRCRTTTAPWFEDEEGYRAARGEDGKTYYVPSSMKYN
                     EWYEKYVKNNSKQTGAKYTKGDIEWNIRREEEAELYYDNIRNRKDDISKISKNTNWSE
                     KSIGQIKNHIFYNTHIMRDGTRRMLDSDYSMSVAWQRLINGTYEDIDILLLKHEYLES
                     IFEKKYNISNLEAHRMTEKKHDWYKELIKQKGEFEEDDCLNELIRKE"
     gene            complement(9490..>10328)
                     /locus_tag="KR250_003333"
     CDS             complement(9490..>10328)
                     /locus_tag="KR250_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBH0069224.1"
                     /translation="IERGNSFIQEFLYDEYGKMTDIQEGHFRINNKEQGWGKVPFIPF
                     KNNEKCVSDLTFYKSLIDIYDNNISTLADNLDEIQEVIYVLKEYPGTSLQEFIDNIRY
                     YKSIKVDGGGGVDKLEINIPVEAKKELLDRLEKNIIIFGQGVNPESQNTGDKSGVALK
                     FLYSLLDLKCSKTEKKFKKAIRELLWFVCEYLKISGSKSYDYKTVQITFNHSMIINEA
                     EKIDMAAKSTGIVSDETIVSNHPWVEDVNDELERLKKQEDTQKEYDDLIPNNQDGVID
                     ET"
BASE COUNT         3015 a         1985 c         1032 g         4296 t
ORIGIN      
        1 ctacaatcta ttttgcggta gagtgaagtt cataattaaa tgaatctatt tgaacttatg
       61 gaacttgatt ttaaaatcaa attcccagcc acttttactc ttgccacgag ttgagtggct
      121 ttttactttt agaaatactt tacaaattaa gcaaaatatt aaactagcaa taacgccagc
      181 tattacatta agtaaaaagt tattcatact tcccacctcc tttcattagg aagtaggttt
      241 tatcccagta tgaactccac tctataaatt gtagattaca tcttcttgct aaaaatatta
      301 taacatataa ttattacata ttttacctat tctatattta tttttttatt ttgctatctt
      361 cttcgtcccc tctttctctc tctttcagct tctttcattg cttcctcttc atcttctatc
      421 tttataagta ttgaggcggc tgctaacgct ctctcattaa cttctaaatt catatattca
      481 cttggcttcc actttaattt ttgaatacaa taatgagtga tgctagcatc aaaatcgcca
      541 cctctgatta gttttttgct tcttctactt tatcctcaaa agatgtatca aatccattga
      601 cttcattcac ttttactgta taattgacat actcacctgc tgtaagcatt gtctttaata
      661 actgagcttc tcccattact ccataactat tttggagttc ggcatccttt aaatctggaa
      721 atactgtaga tgctacacat aattcagcta cataactatt gtagtcaatt tcacttgtat
      781 attgtccagt atgcttacca ttgttaccaa tcacttttac tcttttagta cactttcttc
      841 ttagtgcttc gtcttcttca gatgataaaa cttttaattc ccattcaact ggtttccctt
      901 ctttatctaa aaatctgtta ctcgctacat attttacatt atcaaccttt attgcatttt
      961 gacttaaaaa agcacttaaa ttactcatat tattctaatc tccttttatt ttaatttttc
     1021 atataaaaaa tacacatata taatttataa atgtgtattt tactccattc ctgccaataa
     1081 attaaatttt tctactaatt cccaatcctc aaaagtgaaa tccatatctt catctaaata
     1141 ctcaccatca gcatcaaatt tagtaattat tccactgtcc atattacaat ctttaagtac
     1201 tactgtctgt cttcctacag cagatgtagg gtcttcattt gtaacttgta tgtcaaaata
     1261 aatatcctca ccagtttctt tatatctgta aagtaattct ctaaaaatag aagtattata
     1321 atgaaatgtt gcacttccag tatttgtact cccagttgtt ttatttccct ttgttgttct
     1381 tcctagaatt ggaacttcac ttttattttt ttccatttta gcctctaaat ctatagcttg
     1441 cataaaatta tatcttttac cttctatagt tataaaacat tcagctttct ttgcactaac
     1501 tgtatcttta gcatttattg tttgagccat attatcactc tcctctctaa ttaactgaaa
     1561 cagtcatata aagcttactc atagcattta ttaccttaac agcatcagat actatgacag
     1621 ttttcttatc atttccaagc tctacactaa catcatcagt tttaaaatct tctattgccc
     1681 ttatattctc taattcttta tggtgtttaa caacatcatt ccagaaactt attcttcctg
     1741 ccttatcatt cggaacttta cctaaatact tttcattaaa taaagttgca atatcattag
     1801 caatttggtc aagtactcta acactttggt tacttgaaaa atcgtcattt ttatcatctg
     1861 taaatgatac aaaagtattt atatcctcta acacatgaac ttcatctccc accttgtgga
     1921 atataaattt accagttttt aaagcttctt caagttgtat ttgtgtataa ttaacatcaa
     1981 catcaaactc accatcatac tttttattag tattagattt atttatatcg catcctgcta
     2041 taactccagc cgcccaataa attaaactag attctactaa tccaatatct ttaatcttat
     2101 tttctacaga cactacacct tcataatctg catcactttt cttatatagt actgtttgaa
     2161 acttagctcc taccttatct ctcattctct ttgtaaattc tacaaataaa cttttaattt
     2221 ctgttgttgt agccaaacat cctaaagcat taaagctata gctttctatt ttatccaaga
     2281 aagcttggta ctctgctcct gtcacagctt cgccattagt tccaccagta aatacaagtc
     2341 ctgcacttgc ttctagtgtt gcatccttct tccaagtgat atagtcattg tcttgtaagt
     2401 ctgtaataac ctttgctatt tgagtatcta ccttcttatt atctaaaagt gttacaacat
     2461 caaacttagc attatcatct atatttgttg taactgttac ttttaagtca tttcctctga
     2521 taccactata ttttgctgtg gctatagtac aactggcttt aacgccttta tttaatttat
     2581 aaaaatatcc caaccttata tttttgaata aatctctcaa acctttcagc ttctcatgag
     2641 tataatcata tccaaaatac ttcactgaat acttctcaaa atcatcactg gttacttgaa
     2701 atacgtcttc atctatgccc caatctaact ctaaaggtat tgcaacaata cctctatccg
     2761 ataatgaact ggttgccctc ttagctgaga taaaatttat atagctacct ggtaatattt
     2821 tattctgtgt tacaaatgtt cctccaccta acgccaaatt aactcactcc tttcataaag
     2881 ctatttatta tttcctctac ctctgagaag gaatataact cattttcttt taaaattgca
     2941 tttaataagt cttttctatt tatatacttc ttagaattaa ctatctgctc cttagtaaac
     3001 ttgtaatcgg ttcctttact taatgtctta ctcaaaatta tcacctctct tcaaaccacc
     3061 gaataactct actgtatcca tcttattgat atcattattt tttatagtaa agtagttata
     3121 atcaacaaag aaatgaagta cattatctac aatttcaaag ttcatatttg tacctctgac
     3181 taaatctcca tcaatttcta tatactctaa ttcctccagt agcatctcag ctacctcatc
     3241 tatttcaaat gatttatcat tactttttgg aaaataatgt acatcaaaag agttcttttt
     3301 taatgtcctg ccacttggat aggatacttt gcttggattt aaaggaacaa taaaaaaaca
     3361 aggttcattt ataccttgct ccacatcttc actataaatt gtataactct ctccaaatgt
     3421 tttatctaat ttaatagata ttccatcaat tatattatta agcatcaaat actcctttaa
     3481 gcaatattaa tagttttttc tctataatct tatcaacttg gctttgtagt tccatctctg
     3541 aaattgttaa gaaatgttgt cctttaaccc aaccttttcc atctttagtt ctatggccat
     3601 attcaacata tgcattttgt tatcctaaag gctttttatc ctctagctct tatagtttcc
     3661 tataagttcg gcgtacatca tcaacaaaat aaactttatt tagttgccca gcactcttgg
     3721 agagattata tttattcact ctctacgctc tacggaaacc tatagcctat tcgcaatcta
     3781 taggttttcc tcggtattgg catatataat taattaaata tttttccatg ttcttctatg
     3841 gactatattt gatatagatg aatatgttac aggatatatt tcacttagtt gttttattgt
     3901 atatccatca gaatactttt ttctaatttc ttttacatcc tcaatagata acttagctct
     3961 gctttcttca ataatcttaa ctttttctat aagattattt cctactaatc ccttagaata
     4021 tctacttctt aagcaagaat atgaaatacc tgttttttca gaaagctcta tcaatgtaat
     4081 ttctttttct tcatgcttta ctaatatatt tgttgaccta tttctacatt gtgtctttat
     4141 atccacccat ctacaattgc ttgattcata atttccttca ttatttattc tatcaattgt
     4201 aagtttctct gaatatccat tactcaatgc ccaatttgca aaattatcat agcaaaacca
     4261 ttcatcacac acaattatat tcctttcacc atatctattg tatcttttat tatttttatc
     4321 tgtacatcta gatattatac ccttccatat actgtaaagt cttttatttt gaaccttata
     4381 tttcttttta aattggtatt tgtctgttaa atttaattta tcttgttctt tttttaaaca
     4441 tccacaagat tgtactaagc cactagttaa agagtcagtt cttatttctt tgaaatttcc
     4501 acaatcacac ttacataacc aatattttct atttctttta ccacttttta tctctttaga
     4561 aaatttaatg actcttagcc ttccaaattt ttttcctgta atatctaaaa attttgccat
     4621 aaaatcacct cttttataca ttataaatca agagttattc tatgtccata ttctacctac
     4681 tttataatgt aacactatta cacttagcgt ttaccgattt tgctgggttt tatatgcccc
     4741 attgtgttaa ggcatattca gtcggattaa caacctctat aatataatta tttccttgtt
     4801 tatacacagg aagcgaccta gcataagcca ctccattcca tccttgtcgt aagaatcctg
     4861 tatcaactgg tgttcttcta attactttcc caagtaatcg tgctgctaat tctcttgctg
     4921 catccttgca aaacttatct aaatcaatct ttgtaagctc ctccatcttt ttacaaactc
     4981 ttttaaactc tctaaaatca acactgcccc atctagccat tatgctttat ccttaaataa
     5041 ctcaagtatt atttcttgat gatttggata tatagctgat tctccacttc ttacatactc
     5101 tttatcattt ataataagtt ttgaacctgc tttaatttct atatctggag atataaagag
     5161 tttaatagtt tgctctagct tagctacttt cccttctgta gcagaaacta tatttttata
     5221 tgaaagttta catggttgat tttctaatac aatcacttct ttattgttag ttcgttttgt
     5281 tacagggtct ttaattggct gatactcaac tatagtacat ttatctctat ataacatttc
     5341 tattgctttt ctagttttac ttaccatctt aagcacctaa aggttaatat cttattcttg
     5401 ccataagtag taagataagc tattaagcta tcaaagcgtt gttctggtgt ttgagaacca
     5461 cttcctatag caaaatctac ctttgtatca ccttctgata tagacttttc tacagcttca
     5521 aagttaatgc tttctatatc taattgaccc atatttttct tggtaaataa gaactctcca
     5581 actatcatat caacttcaat ttctttcaat tcaattggca tagtttttat attacaatct
     5641 agtttaataa tattttctat tttttctctt acaaaaccta ttaaccactt atctccatct
     5701 tttaatatat atccaaaact ttcaagtctt ttttctattt catcaattat attattttcc
     5761 ataattttca cctacttttt agtaagttta tttttctctt taagctgctt attttcttct
     5821 tctaaagact caacttttga ccttaaaata ttattttcag ctattaaatc ttttacactt
     5881 aatgacttgc catactttac tgccttacca gtttcatcta tcaaatcata tcccatctct
     5941 aagaaatcat ctattttaca ttcttctatg gttagtattc tatttagttt ttttacttgt
     6001 ggcactatat atcactcctt tttctatgct tcaacaacaa attgtattgc atcagctttt
     6061 ttatttaata taaatacatc ctcaaaactt tcttcaaagt agaagaattt tccctctgta
     6121 actgctgttg gttcgtctaa cttagagaac tgataagaaa caggtgtaat tattgcactt
     6181 gggtgaacta aggacataaa gatttgttta gctcctgctc ctactttcca tccagttgta
     6241 aaatcatatg cagttttcat tagattagat ggtactttaa ttattttaac tgtgtcaata
     6301 tcagttgttt gacgattaag agaagttcct gcatccttta tatttactgt tctttgtatc
     6361 tcttttgcat ttttgataag tgtatttact actggagtaa catacaatat tcttccattt
     6421 tcaggtactc tagcttctgt cattttttcc attaacttat caaatacttc taatacgttt
     6481 gctgttgtaa gaacagttgt atctgctgtg ttacctaatg cggtccaatc agcatatatt
     6541 ttagatatac agtaagcatc catctctgga aacttttgtt cctcattata tacttttgtt
     6601 atattgccta ttgaagccac ataattagtt tggtttatat ctgctggatg aaccaatgta
     6661 gaccatttcc tttgattagt taatacctta ggttcccaag cattatcata gtttctttga
     6721 gctactgcta ttgtatctct gtttgaatct actcttccag ttgtagatat agttggtatt
     6781 tctattgttt tagaaccagt ccatctatat cttccattat ttggtgttgc atacaaatcc
     6841 ccgaagttta aagtataagg atatgcttgt gctaaaacat ttgaatattc ttttgcataa
     6901 tttagtgctg ccattttatt tcctcctatt tattattatt ttcatgaggt cttaccccag
     6961 taaaattaaa accaaaatca tttatcttag gctcttgccc tggtgttata gtatctattt
     7021 taggttcttc accttctagt gttgcattaa acaaataatc tttatcctgt ttcaaaggat
     7081 ttatttgctc ttcaaaagct ttttgtctgt ctttactatt tcttagtgct tctatatcta
     7141 aatgagcttt taatgctatt tcatctctac atttaacaga tttaaaagca tcacctaacc
     7201 agtaattaaa atctttttct tcaatttctt ttttgtaagt ttcttccaaa gttttcttat
     7261 cagtttcata agttgttttt agattctcta catcttcttt tgtcatacct ccttcaaact
     7321 ttttaatagt ttcattagct gtattaagct gtgtttcaag atttgtataa tcttcttgag
     7381 taattgtagt ctcttttatt ttcttttcta tagatttttg aagagaaact acatcaattt
     7441 tgttattttc tatttttatt ccttctagca attcttttaa ccaatccatc ttttaaatat
     7501 ctcctttcat tttttacaca ataaaaaaag tctttaaaga cttttgtcta gtgattcatt
     7561 taattcttct cctactctct ttagaatttc atcagaaact ttatctatat tagcttcatt
     7621 tttttctttt aatttattgc aaagaatacc tacattgaca ttagcaatac aattaataaa
     7681 gatttgtact acacaaacta cagctataac ttgagttaat atatacatta aaatcaccta
     7741 cttttttaaa cttttacata ataaaagcac ctactaattt attgttcagt aagtgcttct
     7801 aacattgata actcatttct tttttcaaat cgttattttt agcagctttt ataagtttag
     7861 aaatagcttt taacgttgct gatttactta tatgttcatc ttttgactct tttattattt
     7921 cataattata aaaatcatct aaatatattt ttattcttcc atcaagctta ttttcttcat
     7981 atccataact atatataaca tattcattat tcttttctaa taagttcatt aagacaatca
     8041 tcttcttcaa actctccttt ctgtttaatt aattctttat accaatcatg ctttttctca
     8101 gtcattctat gggcttctaa gttacttata ttatactttt tctcaaatat actttcaagg
     8161 tattcatgtt ttaataagag aatatcaata tcttcgtatg taccatttat aagtctttgc
     8221 caagcaactg acatactata gtcagaatcc aacatacgtc tagttccatc tctcattata
     8281 tgagtattgt agaaaatatg atttttaatt tgacctatac ttttttctga ccaatttgta
     8341 ttctttgata ttttggaaat atcatctttt ctatttctaa tattatcgta atatagttct
     8401 gcttcttctt ctcttcttat attccactcg atatcacctt tagtatattt tgcaccagtt
     8461 tgtttactat tatttttaac atacttctca taccactcat tatacttcat actagatggt
     8521 acataatatg tttttccatc ttctcctctt gctgctctat agccttcttc atcctcgaac
     8581 caaggagctg ttgttgtcct acaacgacaa tgaaatggtg gagctgttat tccaacttga
     8641 taatctttca tatcaaatat ttttccatct aactctctgc atatatttga agttcttaaa
     8701 tctaatgtag caataatctc atatttctct acttctaaat cactaaaaca atcttttcta
     8761 cttgctgatg caaagaaagc tgattcagtc attatcaaat tcttagcttg tgatttagat
     8821 acattaaatc tcttagcaaa gtcatttact aaattttttg gattttcacc tctaataatt
     8881 gattgagtta gcttagtatg tagttcattt actaaagtag gtctatactt accccaaatt
     8941 ctttcactaa agtttaatcc atctattgcc catggtttag agataatttt atttattcta
     9001 ttagtatcaa gactcattaa actccaacca acgtttactc cttgttgaac attaaaagct
     9061 gtatgatagt atccacttgt ataaatatct ctcattagtt tatcaatacc atcaagttca
     9121 tttccatata aaacttctac ttgttgctgt atttgtaact ttaaagcttc aagccttgtt
     9181 atatgaactc ttgcactagc attttctaac tctttcatcc acttttgatt tatagcattt
     9241 tctttaccat atttaatata ttcttcaaca ctccatttaa actcttctag ttctcttgta
     9301 tttagtagtt tcttagcttc taataaagat attccttcat ttttggcaaa tctgttgtac
     9361 catgctaata tatctttttc tatactattc atagctagtt tatattgctt ttctaattca
     9421 agataatatt ttacactttt gttattttga gcttcttcta attgttcaaa tctctttctc
     9481 caataatctt tatgtttcat ctataacacc atcttgatta ttaggaatta aatcatcata
     9541 ctctttttga gtatcttcct gttttttaag tctctcaagt tcgtcattaa catcctcgac
     9601 ccaaggatgg ttagaaacaa tagtttcatc tgatacaatt ccagttgatt tagctgccat
     9661 atctatcttt tcagcttcat ttattatcat agagtgatta aaagtaattt gaactgtttt
     9721 ataatcatag ctcttactac cacttatctt taaatactca cacacaaacc ataaaagctc
     9781 tctaattgct tttttaaact tcttttcagt cttagaacat tttaagtcaa gtagtgaata
     9841 taaaaattta agtgctacac ccgatttgtc acctgtgttt tgagattctg gattaactcc
     9901 ttgaccaaag ataattatat tcttttccaa tctatcaaga agctcctttt tagcttcaac
     9961 tggtatattt atctctagtt tatcaactcc acctccacca tctactttaa ttgatttata
    10021 gtatcttata ttatctataa actcttgtag acttgttcct ggatattctt ttaatacata
    10081 aataacctct tgtatttcat ctaagttatc tgctagtgta gaaatattat tgtcatatat
    10141 atctattaat gatttataga aagttaaatc tgagacacac ttttcattat ttttaaaagg
    10201 tataaatgga actttacccc atccctgttc tttgttattt attctaaaat gaccttcttg
    10261 tatatcagtc atttttccat attcatcata taaaaattct tgaataaaac tattacctct
    10321 ttcaataa
//
LOCUS       DAEPIN010000101         9674 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.103, whole genome shotgun sequence.
ACCESSION   DAEPIN010000101 DAEPIN010000000
VERSION     DAEPIN010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9674
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.103"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            479..2557
                     /locus_tag="KR250_003334"
     CDS             479..2557
                     /locus_tag="KR250_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HBH0069225.1"
                     /translation="MIKISEDYIEKRFDNELLRIEAWGKNSLRIRSFVDQNFVDENYA
                     LNEKPKLNKDDITINKNEDGSAIIKNGKIKAVLDHRDRITFYNDKNEILLKEYIRLRA
                     VKHDDGGEDVGTIEITKDFNSTLKLKSREYRPSYSGEFEVTTRFESEPSEKIFGMGQY
                     QHKFLDLKNTVLELAQRNSQISVPFYISSLGYGLLWNNPGIGKVSFAKNMTEWKMFST
                     NFIDYWITCGESPKELNKNYSQVTGTVPMMPENLLGLWQSKLRYRTSEEVLDVVKEYS
                     KRGIKLSSIAIDYFHWPKQGEYKFDLDYWKNPKELVKKLKEEYSVEPIVSVWPTVQSD
                     AENYNDYLENGYLVNVNRGVRMTMQIQGNTVFVDMTNENAREYVWDRIDKNYKQLGID
                     YYWLDVAEPGYSVYDFDNYRYKKGNVLSCGNIYPIDYLKMIYDGLHNDIESVVTLVRG
                     AWAGAQKYGALVWSGDIDSSFEAFNNQVNTGLNMGLAGIPWWTTDIGGFHGGNPKDPE
                     FRELMVRWFQYATFSPILRMHGDRLPHSKPLSNKGGGSMVTGAPNEIWSYGEEVEVIL
                     TKFIKIRESLKTYLKKLMKEAHEDGTPVMRTLFYEFPEDDKTWEVDNTYMLGDEILVA
                     PIMNYKDRSRKVYLPKGHTWENIFSGVSYEGGKTYEVECPLEEIPIFLKQDSSYNFKE
                     LKNILGEVKS"
     gene            2557..3336
                     /locus_tag="KR250_003335"
     CDS             2557..3336
                     /locus_tag="KR250_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBH0069226.1"
                     /translation="MEPQLWQLLLIVLYGFFINYEKNSTMFGTYQPVTAGFITGLILG
                     DINTGLYIGGTLQLLSLGISNFGGASIPDYQTASIVATFITITTKQEASVGISIGIPV
                     ALLMVQLDVLRNTIGIWLVHKAEDGAKKGNYKNITYMQMLGVLLTAATTGIPVALSVI
                     FGPSLINTILKYTPEWLTGGLTVAGGLLPAVGIGLLLRYLPAKEYFSYLVIGFVLAVY
                     MKVPLLGVALIGGAIALIIYKKNLENQEQQYTVVGGMDEDE"
     gene            3329..4138
                     /locus_tag="KR250_003336"
     CDS             3329..4138
                     /locus_tag="KR250_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBH0069227.1"
                     /translation="MSDKVSLNKSNEKVKKSVLWRWFFTSSVSSNYEKMQALAYCYAV
                     LPFLKVVYKDNPEKLQSAVLNHLQFFNTNPWVAPYILGINVAMEELSSEETEEAVASI
                     KTGLMGPVAGLGDSLFVVIPWTIFGAIAANMAIDGSPFGILLWIVVSVALKLLSIPLF
                     NAGYTSGTKLISSIEKSLKILTESTSILGLMVVGALIPSVVKANVALNFQQGDFTMKG
                     QEILDQIMPGLVPAILVVIVYWALKKNIKPIYLILGVMVVSIILYALGILK"
     gene            4158..4640
                     /locus_tag="KR250_003337"
     CDS             4158..4640
                     /locus_tag="KR250_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBH0069228.1"
                     /translation="MEMKGIKNIRIDDRLIHGQVATMWSNKLGVTRLMVVNDAVANNS
                     VQKQVLRMATPAGIASSIITEETAIKNITAGKYEGQNVLLIVKSPVDLIPFVEAGFKI
                     HNINVGNMSSRKDTTVLRPNISVTEEEKEAFKRLLDEGIEITTIMTPDDKKTYLVDIL
                     "
     gene            4661..5065
                     /locus_tag="KR250_003338"
     CDS             4661..5065
                     /locus_tag="KR250_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBH0069229.1"
                     /translation="MNSIILMSHGNMAEEVLNSAKMIIGDSIDYPTVNMKQDDGIEGT
                     IEKLKKALEVYKESSEIIIMADLLGGTPCNAALLVANNDSRIKIISGLNLGMVIESAF
                     LDTKELAAKLEKIGKDNISMVEQNASLLSDDE"
     gene            complement(5221..6381)
                     /locus_tag="KR250_003339"
     CDS             complement(5221..6381)
                     /locus_tag="KR250_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HBH0069230.1"
                     /translation="MVTDKYTIREMNERLVLEQIIKNGPISRASIASTIGLNKATISA
                     ITKKLIDESLVHEIGIGNSTHSGGRKPILLVFNKCAGISLSMDIGYDYIFSSLSYLDG
                     TIINSKKLTDIQVSKDNVIQLIDEIINSYNISKIDTPYKVIGLTLAIHGITCENKVLF
                     TPYYNLNEIDLYSILSKKYDFPIHIENEANLTALAENTFSTVHNSLLSLSIHSGFGSG
                     IIINNKLYSGRNGMSGEIGHTIIMPNGKLCPCGNRGCLEQYCSEKKVFEQLSSLENIP
                     KIDSDIVKQLYYEDNHNAKKVIHEFCSYLTIAINNAITTYAPEIIYLNSQIISDIPEI
                     LQITKDMLVSSFNKGINIEISSLGSEASLYGGSAVNIKSFLNIQNLTLINEI"
     gene            6589..8139
                     /gene="xylB"
                     /locus_tag="KR250_003340"
     CDS             6589..8139
                     /gene="xylB"
                     /locus_tag="KR250_003340"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HBH0069231.1"
                     /translation="MKLEYVLGVDIGTSGTKTVLFDKLGNTIKSCTYEYPLIQEKSGW
                     AEQDANDWWKAVVESIREVVQSSNISSECIKGIGLSGQMHGLVMLDNEGKTLRNSIIW
                     CDQRTVKECEEITDLVGEERLIEITANPALTGFTASKILWVRNNEPDIYKNTNKILLP
                     KDYIRYKLTGEYATEVSDASGMQLLDIRKRDWSDEVLEKLNIDKNLLGKVYESQEITG
                     YVTRDVASLTGLKEGTIVVGGAGDQAAGAIGNGIVKDGVVSSTIGTSGVVFAYTKEPK
                     IDKEGRVHTFCHAIPNTWHVMGVTQGAGLSLKWFKDNFCQSEVEVSNSLGEDVYEIIN
                     NQVSQVPTGCNGLLYLPYMMGERTPHLDPYARGVFFGLSPIHSKKEIARSIMEGVSYS
                     LKDCMDIIENLNIDVNEVRASGGGGKSKVWRQMQADMFNQDVYTINSSEGPALGVAIL
                     ALVGAGIYENIQKACDAIIKTSTKLEPISENVDTYNIYHRLYKRIYKSLKDDFKLLDE
                     VVNAKINN"
     gene            8235..9572
                     /gene="xylA"
                     /locus_tag="KR250_003341"
     CDS             8235..9572
                     /gene="xylA"
                     /locus_tag="KR250_003341"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HBH0069232.1"
                     /translation="MSEIFKGIGQIKFEGVKSDNELAFRYYNPEQVVGNKTMKEHLRF
                     AMSYWHTLCGEGNDPFGVGTVERPWNNITDPIEIAKIKVDAGFEFMSKMGIEYFCFHD
                     RDIAPEGRDLEETNKILDEIVEYIKVNMEKTGIKLLWGTANMFGNPRFVHGASTTCNA
                     DVYAYAAAQVKKAMEITKYLGGENFVFWGGREGYETLLNTNTELEMDNFARFLQMAVD
                     YAKEIGFTGQFLIEPKPKEPTKHQYDFDTATVLGFLRKYNLDKYFKMNIEANHATLAG
                     HTFQHELNIARINNVLGSIDANQGDLLLGWDTDQFPTNIYDATLAMYEVLKQGGIAPG
                     GFNFDSKVRRASFEVEDLFLAYIAGMDTFAKGLLIAHKLLEDEVFENFTKERYASFSE
                     GIGKDIVEGKVGFKELESYALQMPVIKNKSGRQEMLEAILNRYIYEVDTISNK"
BASE COUNT         3666 a         1114 c         1801 g         3093 t
ORIGIN      
        1 agttttattt atttgttgtc gtttttgact tggtatacaa ttgtatattt tcttaaataa
       61 tttaataaat tatttataat tataaaatcc attgttttga acaatatcta atatattatt
      121 atctgtattt ataaggtcac tatacatatt ttcaagtccg atattatcta cataataggc
      181 aaatgttatt cctgtatgtg ttttaaaaaa actagatgta tataggtatc atcacagata
      241 ttaaaagtat aaataaaaga tattaagaac gactttccag caatatatta gaaaatttta
      301 ctaaaatccg gaaagcgatt gacaaacaaa gtcaaataaa gtatattatt ggtaagttag
      361 tttaatgtac aaatgaatta tatataaaac ctaatttata aataacgata ctaaacgaca
      421 ttatttaact taagctacac aaagatagaa tattatacca ttgatttagg aggtagccgt
      481 gataaaaata tctgaagact atattgaaaa aagatttgac aatgaactat tgcgaataga
      541 agcatgggga aaaaactctt tacgtattag gtcgtttgta gaccaaaatt ttgttgatga
      601 aaactatgct cttaacgaaa aacctaagtt aaataaagat gatattacaa ttaataaaaa
      661 tgaagatgga agtgcaatca ttaaaaatgg aaagataaaa gctgtcttag accatagaga
      721 tagaattact ttttataatg ataaaaatga aatattattg aaagaatata ttcgattaag
      781 agctgtaaaa catgatgatg gtggagaaga tgtagggaca attgagatta ccaaagactt
      841 taactctact ctaaagttaa aatcaagaga atatcgacca agctatagtg gagaatttga
      901 agtaactact agatttgaat ctgagccttc tgaaaagatt tttggaatgg gacaatatca
      961 acataaattt ttagatttaa agaatactgt tttggagtta gcccaaagaa attctcaaat
     1021 atcagttcct ttttatattt ctagtctagg ttatggactt ttatggaata atccgggaat
     1081 tggaaaagta tcttttgcta aaaatatgac agaatggaag atgttttcta caaattttat
     1141 agattattgg attacttgtg gagaaagccc aaaagagtta aataaaaatt attctcaagt
     1201 tactggaact gttcctatga tgcctgaaaa ccttttagga ctatggcaaa gtaaactaag
     1261 atatagaact tcagaagaag ttttagatgt tgtgaaggaa tattctaaaa gaggaattaa
     1321 gctttcatca attgcaattg actattttca ttggcctaag caaggtgaat ataaatttga
     1381 cttagattat tggaaaaatc ctaaagaatt agttaaaaaa ttgaaagaag agtattcagt
     1441 agaaccaata gtatctgtat ggcctacagt tcaaagtgat gcagaaaact acaatgatta
     1501 tttagaaaat ggctatttag tcaatgttaa tcgtggtgtt agaatgacaa tgcaaattca
     1561 aggaaataca gtctttgtag atatgacaaa tgaaaatgca agagaatatg tttgggatag
     1621 aatagataaa aattataaac aacttgggat tgactattat tggttagatg tagctgaacc
     1681 tggctattct gtgtatgatt ttgataacta tagatataaa aaaggaaatg ttttaagttg
     1741 tggtaatatt tatcctattg attatttaaa aatgatttat gatggtttac ataatgacat
     1801 agagtctgtt gtaactcttg taagaggagc ttgggcagga gctcaaaagt atggagcttt
     1861 agtatggtct ggtgacattg attctagttt tgaagctttt aataatcaag taaatacagg
     1921 gttaaatatg ggactggcag gtataccatg gtggactact gatattggag gtttccatgg
     1981 aggaaatcct aaagacccag aatttagaga acttatggta agatggttcc aatatgctac
     2041 tttttcacca attttgagaa tgcatggaga tagattgcct catagtaaac cattatcaaa
     2101 taaaggtgga ggctcaatgg taactggagc tcctaatgaa atatggtctt atggtgaaga
     2161 agttgaagta attcttacaa aattcataaa aattagagag agcttaaaaa cttatttaaa
     2221 aaaactgatg aaagaagcgc atgaagatgg aacaccagtt atgagaactt tattttatga
     2281 atttcctgaa gatgataaaa cttgggaggt ggataataca tatatgcttg gagatgaaat
     2341 tttggtagct ccaataatga attataaaga tagaagtaga aaagtatatt taccaaaagg
     2401 acatacatgg gaaaatatat ttagtggagt tagttatgaa ggtgggaaaa cttatgaagt
     2461 tgaatgccca ctggaagaaa ttcctatttt cctaaagcaa gattccagtt ataattttaa
     2521 agaactaaaa aatattttgg gagaggtgaa atcataatgg agccacaatt atggcaatta
     2581 ttattaatcg tactatatgg attttttata aactatgaaa aaaattcaac aatgtttgga
     2641 acatatcaac cagtaacagc aggttttatt actggtctta tcttaggaga tataaataca
     2701 gggttatata ttggaggtac attacagcta ttatcattag gtattagtaa ctttggtgga
     2761 gcatcaatac ctgattatca aacagcaagt atagtagcca catttataac tattacgact
     2821 aagcaagaag catctgtagg aatctctata ggtattccag ttgctttatt gatggtacaa
     2881 ttagatgttc ttagaaatac tattggtata tggctagttc ataaagcaga agatggagct
     2941 aaaaaaggaa attataaaaa tataacctat atgcaaatgt taggagtact attaacagca
     3001 gctacaacag gtattccagt tgctctatct gtaatatttg gaccttcttt aattaatacc
     3061 attttgaaat atacacctga atggttaaca ggtggtttaa cagtagctgg aggactactt
     3121 ccagcagtag ggataggatt attacttaga tatcttcctg ctaaggagta ttttagttac
     3181 ttagtaattg gttttgtttt agcagtatat atgaaagtac cattattagg tgtagcttta
     3241 attggtggag caattgcact tataatttac aaaaagaatt tggaaaatca agaacaacaa
     3301 tatactgtag taggaggtat ggacgaagat gagtgataag gtatcattaa ataaatcaaa
     3361 tgaaaaggtg aaaaaaagtg tcttatggcg ctggtttttt actagttctg tttcttcaaa
     3421 ttatgagaag atgcaggcct tggcatactg ttatgcagta ctaccttttt taaaagtagt
     3481 atacaaagat aatccagaaa agttacaaag tgctgtatta aatcatttac aattttttaa
     3541 cactaatcct tgggttgccc cttatatttt gggtattaat gtagcaatgg aggagttatc
     3601 aagtgaagag actgaagagg ctgttgcttc aattaaaaca ggcttgatgg gaccagtagc
     3661 aggtttagga gatagtttat ttgtcgttat tccgtggaca atttttggag ctatagcagc
     3721 taatatggcg atagatggaa gtccatttgg aatcttactg tggattgtag tgagtgtagc
     3781 attgaaatta ttaagtatac cactatttaa tgctggttat acatcaggta ctaagctaat
     3841 atcttcaatt gaaaaaagtt taaagatttt aactgagagt acatctattt taggtttaat
     3901 ggttgtaggt gcattaatac cttcagttgt aaaagctaat gttgctttga actttcaaca
     3961 aggtgatttt acaatgaaag gtcaagaaat attagaccaa attatgcctg ggttagttcc
     4021 tgcaattcta gttgtgattg tttattgggc tcttaaaaag aatataaaac ctatatactt
     4081 aattttagga gtaatggttg tttcaataat tttatatgca ttaggtattt taaaataagg
     4141 aaaggatgat attagatatg gaaatgaaag gtattaaaaa tattcgtata gatgaccgtt
     4201 taatccatgg tcaagttgca actatgtgga gtaataaatt gggtgttaca agattaatgg
     4261 tagtaaatga tgcagtagct aacaattctg ttcaaaaaca agttttaaga atggctacac
     4321 ctgcaggtat agcgtcatct ataattacag aagagactgc tataaaaaat attactgcag
     4381 gaaagtatga aggacaaaat gtattattaa ttgttaagtc tcctgtggac cttattccat
     4441 ttgtagaggc aggatttaaa atacataata taaatgtagg aaatatgtca agtagaaaag
     4501 atacaacagt tctaagacct aatatcagtg ttactgaaga agagaaagaa gcatttaaaa
     4561 gacttttaga tgaaggaatt gaaattacaa ctataatgac gcctgatgat aagaaaactt
     4621 atcttgtaga catcttatag atttaaagag gtgtattaaa ttgaatagta ttattttgat
     4681 gagtcatggc aatatggctg aagaagtctt aaattctgca aaaatgataa taggggattc
     4741 tatagattat cctactgtaa atatgaaaca agatgatgga atagaaggaa caatagaaaa
     4801 attaaaaaaa gctttagaag tgtacaagga aagttcagaa ataattataa tggcagattt
     4861 actaggtgga actccatgca atgctgcttt attagtagcc aataatgaca gtcgaattaa
     4921 aattatttca gggctaaatt taggtatggt tattgaatca gcttttttag atacaaagga
     4981 attggcagca aaattagaga aaataggtaa agataatatt tctatggttg aacaaaatgc
     5041 aagtttatta tctgatgatg agtaggaaga ttatattaat atttaaaacg aagtacttat
     5101 gttaatactt aattgatata caatttttaa aaaatatagc taatcttgca taaactatat
     5161 aataaatcta attttagaat gtataattta aagggttatg gctgtatata gtcataaccc
     5221 ttatatttca ttaattaaag ttaaattttg tatattcaaa aagctcttga tatttacagc
     5281 agagccacca tatagagatg cctcagaccc taatgatgaa atttctatat ttattccttt
     5341 gttaaaagag ctaactaaca tatcttttgt aatctgtaat atctcaggta tatcactaat
     5401 tatttggcta tttaaatata ttatttcagg agcataagtt gtaatagcat tatttatagc
     5461 tattgttaag taactacaaa actcatgaat aacttttttg gcattatggt tatcttcata
     5521 atatagttgc tttactatat cagaatctat tttaggaata ttttctaaag aagaaagctg
     5581 ttcaaatacc tttttttctg aacaatactg ttctaaacag ccacgatttc cacaaggaca
     5641 tagtttaccg ttaggcatga tgatagtgtg accaatttct ccactcatac catttctgcc
     5701 actatataat ttattattta ttataatgcc tgaaccaaat ccactgtgaa tactgagact
     5761 aagtagtgag ttatgtacag ttgaaaaagt attctcagct aaagctgtta agtttgcttc
     5821 attttctata tgaataggaa agtcatactt tttgctaagg atgctatata agtcaatctc
     5881 attaagattg taatatggag taaacaatac tttattttca caagtaatcc catgtatagc
     5941 caaagtaaga ccaattacct tataaggagt gtctattttg gatatgttat aactatttat
     6001 aatttcatct atcaattgta taacattgtc tttacttact tgtatatctg ttaatttctt
     6061 agaatttata atagttccat ctaagtaaga tagagaagaa aatatatagt cgtatccaat
     6121 gtccatactt aaagaaatcc ctgcacattt attaaatact aataatatgg gttttcttcc
     6181 gccactatga gtactatttc caattccgat ttcatgaact agagattcat caataagttt
     6241 tttggtaata gcggatatag ttgctttatt caatcctata gtagaagcta tacttgccct
     6301 agaaatagga ccatttttta taatttgttc aagtaccaat ctttcattca tttctcgaat
     6361 agtgtattta tcagtaacca atttgatatt cctccttaaa ataatattgt aatacttttt
     6421 acacaaaaat aaaaggttat tttgcattga caaagataat taaatatttt attattagtt
     6481 cataagttag tttaatatac taacaaaaat aaagcaagta aaatatacct aaaatataaa
     6541 aaaattagga taggaaaacg atagttatga agtggcattc aaggagggat gaagttggag
     6601 tatgtattag gtgtggatat tggtacatca ggtactaaaa cagttttatt tgataaacta
     6661 ggaaatacta taaaaagttg tacatatgaa tatcctctta tacaagagaa atcaggttgg
     6721 gcagagcagg atgcaaatga ttggtggaaa gctgtagtcg aatctataag agaagtagtt
     6781 caaagttcaa atataagcag tgaatgtata aaaggaattg gattatcagg acagatgcat
     6841 ggtctagtta tgctggacaa tgaaggtaaa acattgagaa actctataat atggtgtgac
     6901 caacgaacag taaaagagtg tgaggagata actgatttag taggtgaaga aagactgatt
     6961 gaaataactg ctaatccagc acttacagga tttacagcat caaagatatt atgggttaga
     7021 aacaatgaac cagatatata taaaaatact aataaaatac ttcttccaaa agattatata
     7081 agatacaaat tgacaggtga atatgctaca gaggtatcag atgcaagtgg tatgcagttg
     7141 ctcgatataa gaaaaagaga ttggagcgat gaagtactag aaaagttaaa tatagataaa
     7201 aatcttttag gtaaagttta tgaatcacaa gagattacag gttatgtgac tagagatgta
     7261 gcaagtttga cagggcttaa agaaggaaca atagtggtag gaggagctgg agaccaagca
     7321 gcaggagcta taggaaatgg aatagttaaa gatggagtag tttcatctac tataggtaca
     7381 tcaggtgtag tatttgctta tacaaaagag ccaaaaattg ataaagaagg tagagttcat
     7441 actttctgtc atgcaatacc aaatacatgg catgttatgg gagttactca aggagctggc
     7501 ttgtcattaa aatggtttaa agataatttc tgtcaaagtg aagtggaagt ttcaaattct
     7561 ttaggtgaag atgtatatga gattataaat aatcaagtaa gtcaagtacc aacaggatgt
     7621 aatggattgc tttacttacc atatatgatg ggagaaagaa ctcctcatct tgacccttat
     7681 gcaagaggtg tgttctttgg attatctcca atccattcta aaaaagaaat tgcaaggtca
     7741 ataatggaag gtgtttcata tagtcttaaa gactgtatgg atataataga gaatcttaat
     7801 atagacgtta atgaagtaag agcctcaggt ggaggtggaa aaagtaaagt atggagacag
     7861 atgcaagcag atatgtttaa tcaagatgta tatactataa attcaagtga aggaccagca
     7921 cttggagttg caattcttgc attagtagga gctggaatat atgaaaatat acaaaaagca
     7981 tgtgatgcta taataaaaac ttcaactaaa ttagaaccaa taagtgaaaa tgtagataca
     8041 tacaatatat atcatagatt gtataaaaga atatacaaat ctttgaaaga tgactttaag
     8101 ttattagatg aagtagttaa tgcaaaaata aataattaaa ttagcttaaa aagaaaaata
     8161 aagatagaaa atagttttaa attaagaaaa catactacat atattatatg tgtaaaatat
     8221 taggaggaca tgaaatgagt gaaatattta aaggaatagg acagataaaa tttgaaggag
     8281 taaaatctga caatgaatta gcatttagat actacaatcc tgaacaagtg gtaggtaata
     8341 agactatgaa agaacactta agatttgcaa tgagctattg gcatacttta tgtggagagg
     8401 gtaatgaccc atttggagta ggcacagtag agagaccttg gaataatata acagacccaa
     8461 ttgagatagc aaagattaaa gttgatgcag gatttgaatt tatgtctaag atgggaattg
     8521 agtatttctg ttttcatgac agagatatag caccagaagg aagagattta gaagagacaa
     8581 ataagatatt agatgaaata gttgagtata taaaagttaa tatggaaaag acaggtataa
     8641 agttattatg gggaactgcc aatatgtttg gaaatccaag atttgttcat ggtgcatcta
     8701 caacatgtaa tgcagatgta tatgcatatg cagcagctca ggttaaaaaa gctatggaaa
     8761 taactaagta cttaggagga gaaaattttg tattctgggg tggaagagaa ggttatgaaa
     8821 ctttacttaa cactaataca gaacttgaaa tggataactt tgcaagattc ttacaaatgg
     8881 ctgtagatta tgctaaggaa ataggattca ctggccaatt cttaatagag cctaagccaa
     8941 aagagcctac taaacatcaa tacgattttg atactgctac tgttttagga tttttaagaa
     9001 agtataatct ggataaatac ttcaaaatga atatagaagc aaaccatgca acacttgcag
     9061 gacatacttt ccaacatgaa ttaaatatag caagaataaa caatgtttta ggtagtatag
     9121 atgcaaatca aggagaccta ttattaggtt gggatacaga ccaatttcca acaaatatat
     9181 atgatgcaac acttgctatg tatgaagtgt taaagcaagg tggaatagca ccaggaggat
     9241 ttaactttga ctctaaagta agaagagcat cttttgaggt agaagactta ttcctagctt
     9301 atattgcagg aatggataca tttgcaaagg gattattaat agctcataaa ttattagaag
     9361 atgaggtatt tgaaaacttt actaaagaaa gatatgcaag ttttagtgaa ggtataggta
     9421 aggatatagt agaaggtaag gttggattta aagagttaga atcttatgca ctgcaaatgc
     9481 cagttataaa gaataagtct ggaagacaag aaatgctaga agctatactt aacagatata
     9541 tatatgaagt agatacaatt tctaataagt aattgtgtag taattaaatt tggatgtttg
     9601 aataaaataa caggatatgt tcttgttttt attctaaaac ggacaaagat aagtatttgt
     9661 aaaaattctc ataa
//
LOCUS       DAEPIN010000102         9584 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.104, whole genome shotgun sequence.
ACCESSION   DAEPIN010000102 DAEPIN010000000
VERSION     DAEPIN010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9584
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.104"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            491..1696
                     /locus_tag="KR250_003342"
     CDS             491..1696
                     /locus_tag="KR250_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HBH0069233.1"
                     /translation="MSKKVLIMSASTGGGHNRAALAIKEELTSKTLDGEPIECEIIDS
                     LKLVNNTMDKIISRGYEKSAIYTPKAYGSVYRLSETNLLSKNEFKDNLLITFMAKKFK
                     KLIRSEKPDLIIGTHPFPMIALSTLKKNFNLHNNESNAYTEHFYKHYTNTINVPPLIS
                     VLTDYTTHSTWIQNEIDYYIVGHEYVKELLVFDGVEPSKIRTFGIPVEKSFLSHRDKD
                     IVLSELNLSPDKLTVLLMGGSFGAGNIKETLDELLDTDRDFQILVITGKNESLKEKIE
                     KKLMSRYHDKNVCVLGYTDKMNDILASIDVLVSKPGGLTTTEALLKDVPMIVPYYIPG
                     QEEENLDFLSNCGAALRTTKKYNLSILLKVLIDDPSRLEMLKRNIKSIRKSNSAQNIA
                     NLILNIISG"
     gene            complement(1767..1988)
                     /locus_tag="KR250_003343"
     CDS             complement(1767..1988)
                     /locus_tag="KR250_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069234.1"
                     /translation="MKPIMNLEEHNAMKEKRVKKMKSKDKPKYDDFEDISFSNKKKTK
                     KNVRNKNAEFRNNYKNYQYDYEEDFYEPI"
     gene            complement(2129..2728)
                     /gene="recR"
                     /locus_tag="KR250_003344"
     CDS             complement(2129..2728)
                     /gene="recR"
                     /locus_tag="KR250_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HBH0069235.1"
                     /translation="MQVYTGPITRLIEEFSKLPGVGRKTAQRLAFHIINMNTNDVEAL
                     SKAIIDAKREIRYCSICCNITDTDPCSMCSNKSRDSSVICVVEDPRDVAAMERTREFK
                     GQYHVLNGVISPMDGIGPDMLKIKELIQRLGNQEVKEIIMATNPTIEGEATAMYIARL
                     VKPMGIKVTRIAHGLPVGGDLEYADEVTISKALEGRREI"
     gene            complement(2741..3091)
                     /locus_tag="KR250_003345"
     CDS             complement(2741..3091)
                     /locus_tag="KR250_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HBH0069236.1"
                     /translation="MAKKGFGGGMMPGGGNMNNLLKQAQKMQENMQKAQQELESKEVE
                     ASVGGGAVTVKVNGKKEVIDITIKPEVVDPDDIEMLQDLVLSAVNQALRNIDDIQASQ
                     MSKVTGGMNIPGLF"
     gene            complement(3150..4787)
                     /gene="dnaX"
                     /locus_tag="KR250_003346"
     CDS             complement(3150..4787)
                     /gene="dnaX"
                     /locus_tag="KR250_003346"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HBH0069237.1"
                     /translation="MHKALYRAYRPQKFEDVIGQDHIIKTLKNQIYSDNIGHAYLFCG
                     TRGTGKTSTAKIFSRAVNCLNKINEEPCNECEICESVLKDNTMDVVEIDAASNNSVDD
                     IRELRESVKYSPANAKYKVYIIDEVHMLSQGAFNALLKTLEEPPSYVIFILATTEPHK
                     IPATILSRCQRYDFKRVTVKDMTLRMKKICEDEGIDIDDKALNLIARNSQGALRDALS
                     ILDQCMSFGESKIDYKDVVELMGSVNIEQLFELSQCIVEQDTKKSLEILNEFVLWGKD
                     IRNLINDLIDHFRNLMVCKVSSELDEIISLPEETIEQLKIQSKNIDINDLIRILNILS
                     ITQDDIKSSSNPRVLVEITIMKIAQPMFDESKEALIKRVENLEKMIELGNFKSEKIGN
                     NKEKEYEVDREIDVKQENVVYEDVKNEDVILIESSWKNILKQIKKDKKMPIYALLSEV
                     KSFNVYSNMLYVIFDDKFDFAKTRLSSQDTINYLEKTIRDVLNRSFNVKIVLTSEVKD
                     INLEVKEKKDIGEEILKNIVSEEILEIKDSIDENESK"
     gene            complement(4864..5128)
                     /gene="ffs"
                     /locus_tag="KR250_003347"
     ncRNA           complement(4864..5128)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="KR250_003347"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            complement(5166..5257)
                     /locus_tag="KR250_003348"
     tRNA            complement(5166..5257)
                     /locus_tag="KR250_003348"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5221..5223),aa:Ser,seq:gga)
     gene            complement(5258..5713)
                     /locus_tag="KR250_003349"
     CDS             complement(5258..5713)
                     /locus_tag="KR250_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HBH0069238.1"
                     /translation="MESSFYMKEALKEAYKAYNKKETPIGAIIVKDNQIIARAHNLTE
                     TLKDSTAHAEILAIKQASEKLGGWRLTDCDLYVTMEPCIMCSGAIVNSRIKKLIIGTR
                     HVKNSYIEKQHEFKLDYFNNNNVKVAFDVLQEECSIILQEFFKALRKRD"
     gene            complement(6034..7305)
                     /gene="serS"
                     /locus_tag="KR250_003350"
     CDS             complement(6034..7305)
                     /gene="serS"
                     /locus_tag="KR250_003350"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HBH0069239.1"
                     /translation="MLDIKRIRENLDDIKKAMERRGEREFDLDAVVELDNKRREILQE
                     VEVMKNELNVNSKKIPQLIKEGKDVTEEKARLKELSDKIKGIDEKVKEVEAKMEYTLM
                     RIPNVPHPEVPQGETDEDNVQIRTWGEPTKFDFEHKAHWDIGTGLGILDFERAGKITG
                     SRFTLYRGLGARLERSLMNFFLNTHTAKHGYTEVLPPFMANRNSFIGTGQLPKFEEDM
                     FKIEGLEYFLIPTAEVPVTNIHANEILDVAELPIKYCAYTPCFRSEAGSAGRDTRGLV
                     RQHQFNKVELVKFVKPEDSYNELESLTHDAETMLQMLGLPYRVVKICTGDLGFTAAFK
                     YDLEVWMPSYNRYVEISSCSNFEDFQARRAGIRFKRDKKSKAEYVHTLNGSGLAIGRC
                     LAAILENYQQADGSVVVPEELRPYMGVDVIK"
     misc_binding    complement(7431..7636)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(7764..8645)
                     /locus_tag="KR250_003351"
     CDS             complement(7764..8645)
                     /locus_tag="KR250_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HBH0069240.1"
                     /translation="MNFLSLAKTASTNSNITKRTLENMLDNFSDNIPNIIYAIIVFVI
                     GIYISKIVRRMVSKFLIKYGMSKGVNNFIVYGIYISMLSIISLISLGIIGIQTTSVVA
                     VLGAAGFSIGLAFKEILSNLGSGMIILFFKPFNIGDYIQGSGVEGTVSDIQIFSTVLK
                     TPDNKTIIIPNFQLTSNNIINYTHQNKRRIDFSYNISYDSDIDVVKSILNEIFTNEKR
                     ILNDPKPIIGLNSIGNNTMQIVARPWVKTDDYWDVYFDVMEKVKNKFDENDIKVPFVP
                     SSLLFNNTGNLNTMDKR"
     gene            complement(8657..9166)
                     /locus_tag="KR250_003352"
     CDS             complement(8657..9166)
                     /locus_tag="KR250_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HBH0069241.1"
                     /translation="MNSNERRSKLIDILKESKHPVKGGTLAELLNVSRQVIVQDIALI
                     RARGFEIIATPQGYIIYNQPYFTKQIKCKNHKDSKEIYDELKIIVDLGGIIKDVIVNH
                     PTYGQITAELNIYSKMDIDNFMKKVETNEFKQLSVLTECSHIHTIEAIKEETIEAIVK
                     ELKDNGILS"
     gene            complement(9292..9408)
                     /gene="rrf"
                     /locus_tag="KR250_003353"
     rRNA            complement(9292..9408)
                     /gene="rrf"
                     /locus_tag="KR250_003353"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         3057 a         1678 c         1030 g         3819 t
ORIGIN      
        1 ctcagatact attgttatca aaatatctgg ctttatggtt tgtttcttaa tataaattaa
       61 cctactgttt aattttcaat gttcgtgttt atttcttttt ccttttttca aggacaagta
      121 atactatatc atctttatct attctagtca atataatttt ttactttttt taattttttt
      181 atttatcatg tattctttat ctttaatttt taaaacttaa tgctaagtaa acctgtttat
      241 ttcataaaac taatatatat tctatgctct attttccttt ttattctata tttaaatttt
      301 ttatttacaa gaatattttt aatataacat atttgataaa attaatttac atttctttca
      361 cttgtaattt ttgcaatctt gtattagatt aatattaaaa ataatttatc aataacctta
      421 ttcacaaaga cagtttttat atatcaaaat ataatataaa tactatgcta cattttagga
      481 ggattttatt atgagcaaaa aagtattaat aatgtctgct tccactggtg gtgggcataa
      541 tagggctgct ctagctatta aagaagaact tacttccaaa acacttgatg gagagccaat
      601 tgaatgtgaa ataatagata gtttgaaatt agttaataat acaatggata aaataatttc
      661 aagaggatac gaaaaatctg ctatatatac tcccaaagcc tatggaagtg tatatagatt
      721 atctgaaaca aatcttttgt caaaaaatga atttaaagat aatctactaa taacattcat
      781 ggctaaaaaa tttaaaaagc ttattagaag cgaaaaacca gacttgatta taggaacgca
      841 tccttttcca atgattgctc taagtacatt gaaaaaaaac tttaatttac ataataatga
      901 atcaaatgct tacacagagc atttttataa acattataca aataccatta atgttcctcc
      961 gcttatttct gtattaaccg attatactac tcattctaca tggatacaaa atgaaattga
     1021 ttactatatt gttggtcatg aatatgtaaa agaattattg gtgtttgatg gagttgaacc
     1081 gagtaaaatt agaacatttg gaatccctgt tgaaaaatct tttctctctc acagagataa
     1141 agatattgtt ctttctgagt tgaatttatc tcctgataaa ttaactgttt tacttatggg
     1201 gggtagcttt ggggctggaa atataaaaga gacacttgat gaattactgg atactgatag
     1261 agactttcaa atacttgtaa ttactggtaa gaatgaaagc ctaaaagaaa aaatcgaaaa
     1321 aaaacttatg tctcgttatc atgataaaaa tgtatgtgtc cttggataca ctgataagat
     1381 gaatgatatt ttagcttcta tagatgtatt agtaagcaaa cctggtggac ttactacaac
     1441 tgaggctctt ttaaaagatg tacctatgat agttccttat tatataccag gtcaggaaga
     1501 agagaatctt gatttcttat ctaattgtgg tgcagcactt agaactacaa aaaaatataa
     1561 cttgtcaata ttactaaaag ttttaataga tgaccctagc agattagaaa tgctaaaaag
     1621 aaatattaaa tcaataagaa agtcaaattc agcccaaaat atagctaatt taattttaaa
     1681 tataatttct ggttgataaa ataaataaaa tccctatagt atatatatta tctattaggg
     1741 attttttatt ttctaattat ttaatattaa attggctcat aaaagtcttc ttcataatca
     1801 tactgataat ttttataatt atttctaaat tcagcatttt tatttcgtac atttttttta
     1861 gtttttttct tattagaaaa acttatatcc tcaaaatcat catatttagg tttatctttt
     1921 gatttcattt ttttaactct tttttctttc attgcattat gttcttctaa gttcataatt
     1981 ggtttcatgt tgcaccccta tcaaaatttt taaatgattt atacaacaaa ttatacaata
     2041 cagaagaaat ttattcaata aatctaaatc aaaattaatt tgaatttaac tcaaaaaact
     2101 acaaaaatcg aggtattaac ctcgattttt atatttctct tctgccttct agtgcttttg
     2161 atattgtgac ctcatctgca tactctaaat cgcctccaac aggaagacca tgagctatcc
     2221 gagtgacttt gattcccatt ggttttacta acctagcaat atacatggct gtagcttcac
     2281 cttcaatagt aggatttgtt gccattatta tttctttgac ttcctgattg ccaagtcttt
     2341 ggataagttc ctttattttt aacatatctg gtccaattcc atccattgga gaaattactc
     2401 catttaatac atgatactgt cctttaaact ctctcgttct ctccatagct gctacatctc
     2461 ttggatcttc cacaacacat ataacacttg aatctctact tttatttgaa cacatgctac
     2521 atgggtctgt atctgttata ttacaacaaa ttgagcaata tcttatttct ctttttgcat
     2581 ctataatcgc tttagataaa gcttccacat cattagtatt catattaata atatgaaaag
     2641 ccaatctttg agcagtcttt cttcctactc caggaagctt tgaaaattcc tctataagtc
     2701 tcgttatggg acctgtataa acttgcataa ataccaccta ctagaataac ccaggtatat
     2761 tcatacctcc agttacctta ctcatttgac ttgcttgtat atcatcaata tttctcaatg
     2821 cttgatttac tgcacttaaa actaaatctt gtagcatttc tatatcatct ggatcaacta
     2881 cttctggctt gatagttata tctataactt cttttttacc attaactttt acagtaactg
     2941 ctcctccacc aactgaagct tctacttctt tactttctaa ctcttgttga gctttttgca
     3001 tattttcttg cattttttga gcctgcttta acaagttgtt catatttcca ccaccaggca
     3061 tcattccgcc tccaaaacct tttttagcca taaacactat tcctcctaaa cttattttca
     3121 ttttattatt atatcataat agtattttat tatttactct cattttcatc aatactatct
     3181 tttatttcta atatctcttc actcactatg ttctttaata tttcctcacc aatgtccttt
     3241 ttctctttaa cttcaagatt tatatcttta acttctgatg tcagtactat ttttacatta
     3301 aaacttctat ttaaaacatc tcttattgtt ttttctaaat aatttattgt atcttgagag
     3361 ctcagtctag tttttgcaaa atcaaattta tcatcaaata taacatacaa catattagaa
     3421 tatacattaa aactttttac ttcacttaag agtgcgtata ttggcatttt tttatctttt
     3481 ttaatttgtt taagtatatt cttccaagat gactctataa gtataacatc ctcatttttt
     3541 acatcttcat aaactacatt ttcttgtttt acatctattt ctctatcgac ctcatactct
     3601 ttttctttat tattcccaat tttttcagac ttaaagttcc ccagttcaat cattttttct
     3661 aagttctcaa ctctttttat aagagcttct ttactttcat caaacattgg ttgagcaatt
     3721 ttcattatgg ttatttccac taaaaccctt ggatttgaag atgactttat atcatcctga
     3781 gttattgata atatatttaa aatccttatt aaatcattta tatctatatt tttagactga
     3841 attttaagtt gttctatagt ttcttctggt aatgaaatta tttcatccaa ttctgatgaa
     3901 accttacata ccattaaatt tctaaaatga tcaattaagt catttataag atttcttata
     3961 tctttgcccc ataagacaaa ttcatttaat atctcaagag actttttagt gtcctgctct
     4021 acaatacatt gagacaattc aaataattgt tctatattta cgcttcccat taattcaaca
     4081 acatctttat aatctatctt actttctcca aaagacatac attggtctaa tatactaagt
     4141 gcatctctaa gagctccttg agaatttcta gctattaaat taagagcttt atcatctata
     4201 tcaattcctt catcttcaca aatctttttc attctaaggg tcatatcttt aactgtaact
     4261 cttttaaaat cgtatctttg gcatcttgat aaaatggttg caggtatttt gtgaggctct
     4321 gtagttgcta atataaaaat tacatatgat ggaggttcct ctaaggtctt taataatgca
     4381 ttaaaagctc cttgagacaa catatgaact tcatctatta tatatacttt atatttagca
     4441 ttggctggag aatatttcac actttctcta agctctctta tatcatctac actattattt
     4501 gaagctgcat ctatttctac aacatccatt gtattatctt ttaatacaga ttcgcatatc
     4561 tcacactcat tacacggttc ttcatttatt ttattcaaac agtttactgc tcttgaaaaa
     4621 atctttgcag tagatgtttt accagtacct cttgttccac aaaacaaata agcatgtcct
     4681 atattatcac tatatatttg attctttaaa gtcttaataa tatgatcttg accaataaca
     4741 tcttcaaatt tttgtggtct atatgctcta tataatgctt tatgcatctt tttacctctc
     4801 tttagcaaaa ttaataaaaa aatccctcat tatctaaggg attagtcttt tatatattaa
     4861 atgctgtaca ccatctatcg acttaattcc ataggcgtta ccaaaacagt tagctcaact
     4921 taggcacccc cacggcacac aaaagtgatc acttaccgct gcttccttcc ggatctgacg
     4981 gggttcatga gctttcattg cgcaggacct aagcatcaac accacttatt tagggcggcc
     5041 ctacagaaaa taagccttta gatggaattc aatcctgcta tagcggattg caggttacag
     5101 ggcaccgcta cctccccatc tagtacagca aaattacaac catatttaat tactttcatt
     5161 gtttctggcg gagagagtgg gatttgaacc cacgatacag ttttacccat atactcgctt
     5221 tccaggcgag cgccttcgac cactcgacca tctctcctta atctcttttt ctcaatgcct
     5281 taaaaaattc ttgcaatatg atcgagcatt cttcttgcaa aacatcaaaa gctactttta
     5341 cattattatt attaaaataa tctaatttaa actcatgctg tttttctata tatgaatttt
     5401 ttacatgtct tgtccctatt ataagctttt ttattctaga attaactatt gcgccactac
     5461 acattatgca aggttccatt gtaacatata aatcacaatc agtcaacctc caaccaccta
     5521 atttttctga tgcttgtttt atcgctaaaa tttctgcatg tgccgtagag tcttttaacg
     5581 tttctgtaag gttatgagct ctagctatta tttgattatc tttaactata atagcaccta
     5641 ttggtgtttc ttttttatta tatgctttat atgcttcttt taatgcttct ttcatataaa
     5701 atgaactttc cataattcct cccaaaactt ataacaaact tatcttatca taattcattt
     5761 tataattcaa cttaatttta ttttgtttat ttaattatta ctatatatag ctatattttt
     5821 tttaaattag ttctaaaata atttttagat attcatacaa gagtgtttta taaaaatatt
     5881 tagtgtttta tgatgaaaat tctgctgtgt aaattaacat aaaaaaacta tgaaattaat
     5941 atttataata atttcatagt ttttttattt attgtttact cataatatat tctcttcatc
     6001 aaagcatact ctacatatac aattaatata aatttatttt ataacatcaa cacccatata
     6061 aggtcttaat tcttctggaa ctactacaga accatctgcc tgctgataat tttctaatat
     6121 agcagccaaa catcttccta tcgctaatcc agaaccattt aatgtatgaa catattctgc
     6181 ttttgatttt ttatcccttt taaatcttat tcctgctctt cttgcttgga aatcttcaaa
     6241 atttgaacaa gaagatattt ctacatatct attgtagctt ggcatccaaa cttctaaatc
     6301 atatttaaat gcagctgtaa atcccaaatc tcctgtacat atttttacaa ctctatatgg
     6361 aagacctaac atttgtaaca tagtttcagc atcatgagtt aatgattcta attcattata
     6421 agaatcttct ggtttaacaa atttaactaa ttcaacttta ttaaattgat gttgtctaac
     6481 taaacctctt gtatctcttc ctgctgaacc tgcttcagat ctaaaacaag gagtatatgc
     6541 acagtattta attggaagct ctgcaacatc taatatttca ttagcatgta tattagtaac
     6601 aggaacttca gctgttggta ttaagaaata ctctaatcct tctattttaa acatatcttc
     6661 ttcaaattta ggaagttgac cagtccctat aaaactattt ctattggcca taaatggagg
     6721 taagacttcc gtatagccat gcttagctgt atgagtattt aaaaagaaat tcattaaaga
     6781 tctttctaat ctagctccta atcccctata taaagtaaac cttgacccag ttattttccc
     6841 tgctctttca aaatctaaaa tacctagacc tgtacctata tcccaatgcg ctttatgctc
     6901 aaaatcaaat ttagtaggtt ctccccaagt tcttatttga acattgtctt catctgtttc
     6961 accttgtggt acctctggat gaggaacatt tggtattctc attaatgtat attccatttt
     7021 tgcttctact tcttttactt tctcatcaat accttttatc ttatctgata actctttcaa
     7081 tcttgccttt tcttctgtta catcttttcc ttcttttatt aattgaggta tttttttaga
     7141 atttacattt aactcatttt tcataacttc aacttcttga agtatttctc ttcttttatt
     7201 atctaattcc actacagcat caaggtcaaa ttctctttca cctcttcttt ccatagcttt
     7261 tttgatatca tctagatttt ctcttattct tttaatatct aacatattaa ttcctcctaa
     7321 taattttgat aatataagtt ataattccaa cttaatttac aaacattata cttgtaaact
     7381 cttagtttaa acaaatttta aaaataaaaa aagacctccg tttaccctat aaaagggacg
     7441 aaaatctttt atccgcgttg ccaccctaat tgactatact aaaatgtata atcctctcag
     7501 acctataacg taggtaaacg tctagcttta actagaagct ctagagtaga ttcaaaaacc
     7561 attgtaatga gttttcacca tccactcatt ctctgaatac aatctgtttt ttactatttt
     7621 ccttcacagc tttttaacta attgttttat ttgataatca ttatattata tatattaaca
     7681 tctttcaata ctattattca aattttttta gaattttctt taatttttta tattaaattc
     7741 attttttcta tttaattaag accttatctt ttatccattg tatttagatt acctgtatta
     7801 ttaaataata atgaagaagg aacaaatgga acttttatat cattctcatc aaatttattt
     7861 tttacctttt ccattacatc aaagtataca tcccagtaat catcagtttt tacccaaggt
     7921 ctagcaacta tttgcatggt gttatttcct atagagttta gacctattat aggctttgga
     7981 tcatttaata ttcttttttc gtttgtaaaa atttcattta atatagactt aactacatct
     8041 atatcactat catatgaaat attatatgaa aaatctattc ttcttttgtt ttgatgtgtg
     8101 taattaatta tattattact tgtcagttga aaattaggta taattatagt tttattatct
     8161 ggcgtcttaa gaacagtgct aaatatttgt atgtcagaaa ctgtaccttc tacacctgaa
     8221 ccttgaatat aatcacctat attaaatggc ttaaaaaata aaataatcat accagagcct
     8281 aaatttgata atatttcttt aaaagctaaa cctatactaa atccagcagc tcctaacaca
     8341 gctacaactg atgtagtttg tatccctata atacctaagc ttatcaatga tataatagat
     8401 aacatagata tatatattcc atatacaata aaattattta cacctttact cattccatat
     8461 ttgattagaa actttgaaac catccttctt actattttag atatataaat acctatcaca
     8521 aatacaatta ttgcatatat aatatttggt atattatcac taaaattatc gagcatattt
     8581 tctaaagttc tcttagttat attgctattt gttgaggcag tcttagccaa tgataaaaaa
     8641 ttcattcaac tcctccttat gataatattc cgttgtcctt taactctttt actatagctt
     8701 ctatagtttc ttcttttatt gcctcaattg tatgaatatg gctacattct gtcagaaccg
     8761 ataattgttt aaattcattt gtctcaactt tcttcataaa gttgtctata tccattttag
     8821 aataaatatt aagttctgca gttatctgac catatgttgg atgatttaca ataacatctt
     8881 ttataatacc acctaaatct acaattatct ttaattcatc ataaatttct ttagaatcct
     8941 tatgattttt acactttatt tgttttgtaa aatacggttg attatatatt atatatcctt
     9001 gaggtgtagc tattatttca aatcctctag ctcttataag ggctatatct tgaactatta
     9061 cttgcctact tacatttaat agttcagcta gtgttccacc ttttacagga tgcttagatt
     9121 cttttaaaat atctattaat ttacttcttc tttcattaga attcatatta caccttcttt
     9181 ccaaataaaa actttaccta tactatataa ttttatattt tatcttatta taaatcaata
     9241 ttgactttat attctattta atcttaactt ttataataaa taaaaaagac tacgtggcta
     9301 cgtcctactc tcccaggcag tttcccacca agtaccatca gcgctagaga gcttaacttc
     9361 tgtgttcgga atgggaacag gtgtatcctc tctgctattg taaccacata atctttatgc
     9421 ttaatatttc tattaagttt ttaataagtt tctagaatct agtaatacta gtttctttag
     9481 agttagcact ctaaaaacta cacatatatc ttaatgaatg aacatcaaat aaatattggt
     9541 caagtcctcg acctattagt atcgataagc taaatacatt gctg
//
LOCUS       DAEPIN010000103         9562 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.105, whole genome shotgun sequence.
ACCESSION   DAEPIN010000103 DAEPIN010000000
VERSION     DAEPIN010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9562
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.105"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            <1..848
                     /locus_tag="KR250_003354"
     CDS             <1..848
                     /locus_tag="KR250_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBH0069242.1"
                     /translation="TYFVERGNSFVQEFLYDEYGKMTDIQEGHFRINNKEQGWGKVPF
                     ISFKNNEKCVSDLTFYKSLIDIYDNNISTLADNLDEIQEVIYVLKEYPGTSLQEFIDN
                     IRYYKSIKVDGGGGVDKLEINIPVEAKKELLDRLEKNIIIFGQGVNPESQNTGDKSGV
                     ALKFLYSLLDLKCSKTEKKFKKAIRELLWFVCEYLKISGSKSYDYKTVQITFNHSMII
                     NEAEKIDMAAKSTGIVSDETIVSNHPWVEDVNDELERLKKQEDTQKEYDDLIPNNQDG
                     VIDET"
     gene            838..2235
                     /locus_tag="KR250_003355"
     CDS             838..2235
                     /locus_tag="KR250_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HBH0069243.1"
                     /translation="MKHKDYWRKRFEQLEEAQNNKSVKYYLELEKQYKLAMNSIEKDI
                     LAWYNRFAKNEGISLLEAKKLLNTRELEEFKWSVEEYIRHGKENAINQKWMKELENAS
                     ARVHITRLEALKLQIQQQVEVLYGNELDGIDKLMRHIYTSGYYHAAFNVQQGVNVGWS
                     LMNLDTNRINKVISKPWTSDGLNFSERIWGKHRPALINELHTKLTQSIIRGENPKNLV
                     NDFSKRFNVSKSQAKNLIMTESAFFASASRKDCFNDLDVEKYEIIATLDLKTSNICRE
                     LDGKVFDMKDYQVGVTAPPFHCRCRTTTAPWFEDEEGYRAAKGEDGKTYYVPSSMKYN
                     EWYEKYVKHNSILEIKNSAIIDSIKEDIKNGKYNLNIHDGKQGKHLKEHNNYIEGRSY
                     LTITKEEAQELVNKHAGNGIIKFNRSGEWDKKELIEVDKNIGVNVNNITGEKTLTNKF
                     KIHYSKTGTHIVPAL"
     gene            2250..2450
                     /locus_tag="KR250_003356"
     CDS             2250..2450
                     /locus_tag="KR250_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069244.1"
                     /translation="MKLWEYVGKNVQITCVDKQIIRGKCDGYTQALDNEPEIASISIA
                     RDGYGIEVYENEIESIENINKE"
     gene            2516..3079
                     /locus_tag="KR250_003357"
     CDS             2516..3079
                     /locus_tag="KR250_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage scaffolding protein"
                     /protein_id="HBH0069245.1"
                     /translation="MDWLKELLEGIKIEDNKIDVASLQKSIEKKIKETTITQEDYTNL
                     ETQLNTANETIKKFEGGMTKEDVENLKTTYETDKKTLEETYKKEIEEKDFNYWLNDAF
                     KSIKCRDEIALKAHLDIEALRNSKDRQKAFEEQINPLKQDKDYLFNATLEGEEPKIDT
                     ITPGQEPKINDFGFNFTGVRPHENNNK"
     gene            3091..3990
                     /locus_tag="KR250_003358"
     CDS             3091..3990
                     /locus_tag="KR250_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
                     /protein_id="HBH0069246.1"
                     /translation="MAALNYAKEYSNVLAQAYPYTLNFGDLYATPNNGRYRWTGSKTI
                     EIPTISTTGRVDSNRDTIAVAQRNYDNAWEPKVLTNQRKWSTLVHPADINQTNYVASI
                     GNITKVYNEEQKFPEMDAYCISKIYADWTALGNTADTTVLTTTNVLEVFDKLMEKMTE
                     ARVPENGRILYVTPVVNTLIKNAKEIQRTVNIKDAGTSLNRQTTDIDTVKIIKVPSNL
                     MKTAYDFTTGWKVGAGAKQIFMSLVHPSAIITPVSYQFSKLDEPTAVTEGKYFYFEES
                     FEDVFILNKKADAIQFVVEGAGA"
     gene            3990..4223
                     /locus_tag="KR250_003359"
     CDS             3990..4223
                     /locus_tag="KR250_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069247.1"
                     /translation="MAQVRKLNRILTIEECKIDDFLEMGYDLIDETGKVVRYGKSLNV
                     KDLIAENNILRSKVESLEEENKQLKEKNKLTKK"
     gene            4233..4634
                     /locus_tag="KR250_003360"
     CDS             4233..4634
                     /locus_tag="KR250_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069248.1"
                     /translation="MGNNIIDDIEKRLESFGYILKDGDKWLIDFVREKIENIIKLDCN
                     IKTMPIELKEIEADMIVGEFLFTKKNMGQLDIESINFEAVEKSISEGDTKVDFAIGSG
                     SQTPEQRFDSLIAYLTAYGKNKILTFRCLRW"
     gene            4628..4975
                     /locus_tag="KR250_003361"
     CDS             4628..4975
                     /locus_tag="KR250_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069249.1"
                     /translation="MVSKTRKAIEMLYRYKCTIVEYQPIKDPVTKRTNNKEVIVLENQ
                     PCKLSYKNIVSATEGKLAKLEQTIKLFISPDIEIKAGSKLIINDKEYVRSGESAIYPN
                     HQEIILELFKDKA"
     gene            4975..5400
                     /locus_tag="KR250_003362"
     CDS             4975..5400
                     /locus_tag="KR250_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HBH0069250.1"
                     /translation="MARWGSVDFREFKRVCKKMEKLTKIDLDKFCKDAARELAARLLG
                     KVIRRTPVDTGFLRQGWNGVAYARSLPVYKQGNNYIIEVVNPTEYASYVNFGHRTKDG
                     KGWVKGQHFLTISEMELQSQVDKIIEKKLLILLKGVFDA"
     gene            5393..5830
                     /locus_tag="KR250_003363"
     CDS             5393..5830
                     /locus_tag="KR250_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069251.1"
                     /translation="MLNNIIDGISVKLDKSFGEKYTIYSEDVEQGINEPCFFIVPLNP
                     SKTPYPSGRELKKNSFDVHYFPRSEAKNFEINEIAEMLLEELEYIEINGDLVRGTNMN
                     FEIIDNVLHFFVDYNYFTIKSNNADKMNTVELFGGLKRGDNFE"
     gene            5823..5999
                     /locus_tag="KR250_003364"
     CDS             5823..5999
                     /locus_tag="KR250_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069252.1"
                     /translation="MSKTLSKGTDYKFTKEQIVNSKKYVNRKDLLNAILKENELYSFS
                     EVEDRINKFMKGVS"
     gene            6000..7310
                     /locus_tag="KR250_003365"
     CDS             6000..7310
                     /locus_tag="KR250_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath family protein"
                     /protein_id="HBH0069253.1"
                     /translation="MALGGGTFVTQNKVLPGSYINFVSATRATSSLSDRGIVAMPLEL
                     DWGIDEEVFQVTSDDFEKYSTKYFGYDYTHEKLKGLRDLFKNIRLGYFYKLNKGVKAS
                     CSIATARCSGIRGNDLKVIVTTNIDDNAKFDVVTLLDNKKVDTQIAKVITELQDNDYV
                     TWKKEATLEATAGLTFTNGTNGEAVTGTEYQAFLDKIESYSFNALGCLATTAEIKSLF
                     VEFTKRMRDKVGAKFQTVLYKKNDADYEGVVSVENKIKDTGLLESSLIYWTTGAIAGC
                     DINKSNTNKRYDGEFDVDVNYTQIHLEEALKTGKFIFHKVGDEVHVLEDINTFVSFTD
                     EKNDDFSSNQSVRVLDQIANDIATLFNTKYLGEVPNDKSGRISFWNDVVKHHEQLQNM
                     RAIEDFKADDVSVEPGSDKKTVVVSDAVKVISAMSKLYMTVSVS"
     gene            7332..7802
                     /locus_tag="KR250_003366"
     CDS             7332..7802
                     /locus_tag="KR250_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HBH0069254.1"
                     /translation="MAQQIKARDTISASKAECFVTIKGKRYNFMQAINLEAKMEKNKS
                     EIPILGSTTKGNKSTGSKYSGNATFYYNTSIFRELLYEYKETGEDIYFDIQITNEDPT
                     SSVGRQTIILEDCNMDSGIIAKFDADGEYLDEDMDFTFENWKLVEKFNIANGME"
     gene            7855..8112
                     /locus_tag="KR250_003367"
     CDS             7855..8112
                     /locus_tag="KR250_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069255.1"
                     /translation="MIKIKDKYEIKDSISFDYSNKRPLEERVSEMYKKAGKYLIDISD
                     KLATDTIDGSSLKPIIIKFEINEAGVATIEKQTKYLVMEVE"
     gene            8117..8557
                     /locus_tag="KR250_003368"
     CDS             8117..8557
                     /locus_tag="KR250_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBH0069256.1"
                     /translation="MGDLNAFLSQNAIKVENRKYVASERFIGEDGKAIEWELKAIDSD
                     RDRQLRKDSTIRVPVLNKKGKATGQYTSETDFNTYTLKLCVETVVFPDLHDAELQNSY
                     GVMGAEELLTTMLTPGEYTDLSSEVGEVNGFDRTFEDKVEEAKN"
     gene            8878..9039
                     /locus_tag="KR250_003369"
     CDS             8878..9039
                     /locus_tag="KR250_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069257.1"
                     /translation="MDNFLLSILASLIASLIGYIVCRCIKNVKSHSTRGKSKSGWELG
                     FKIKFRKFK"
BASE COUNT         3935 a          993 c         1838 g         2796 t
ORIGIN      
        1 taacttactt tgttgaaaga ggtaatagtt ttgttcaaga atttttatat gatgaatatg
       61 gaaaaatgac tgatatacaa gaaggtcatt ttagaataaa taacaaagaa cagggatggg
      121 gtaaagttcc atttatatct tttaaaaata atgaaaagtg tgtctcagat ttaactttct
      181 ataaatcatt aatagatata tatgacaata atatttctac actagcagat aacttagatg
      241 aaatacaaga ggttatttat gtattaaaag aatatccagg aacaagtcta caagagttta
      301 tagataatat aagatactat aaatcaatta aagtagatgg tggaggtgga gttgataaac
      361 tagagataaa tataccagtt gaagctaaaa aggagcttct tgatagatta gaaaagaata
      421 taattatctt tggtcaagga gttaatccag aatctcaaaa cacaggtgac aaatcgggtg
      481 tagcacttaa atttttatat tcattactgg accttaaatg ttctaagact gaaaagaagt
      541 ttaaaaaagc aattagagag cttttatggt ttgtgtgtga gtatttaaag ataagtggta
      601 gtaagagcta tgattataaa acagttcaaa ttacttttaa tcactctatg ataataaatg
      661 aagctgaaaa gatagatatg gcagctaaat caactggaat tgtatcagat gaaactattg
      721 tttctaacca tccttgggtc gaggatgtaa atgacgaact tgagagactt aaaaaacagg
      781 aagatactca aaaagagtat gatgatttaa ttcctaataa tcaagatggt gttatagatg
      841 aaacataaag attattggag gaagagattt gaacaattag aagaagctca aaataacaaa
      901 agtgtaaaat attatcttga attagaaaag caatataaac tagctatgaa tagtatagaa
      961 aaagatatat tagcatggta caacagattt gccaaaaatg aaggaatatc tttattagaa
     1021 gctaagaaac tactaaatac aagagaacta gaagagttta aatggagtgt cgaagaatat
     1081 attagacatg gtaaagaaaa tgctataaat caaaagtgga tgaaagagtt agaaaatgct
     1141 agtgcaagag ttcatataac aagacttgaa gctttaaagt tacaaataca gcaacaagtg
     1201 gaagtgcttt atggaaatga acttgatggt attgataaat taatgagaca tatttataca
     1261 agtggatatt atcatgcagc ttttaatgtt caacaaggag taaacgttgg ttggagttta
     1321 atgaatcttg atactaatag aataaataaa gttatctcta aaccatggac tagtgatgga
     1381 ttaaatttta gtgaaaggat ttggggcaaa catagacctg ctttaattaa tgagttacat
     1441 actaagctaa ctcaatcaat tattagaggt gaaaatccaa agaacctagt aaatgacttt
     1501 tctaagagat ttaatgtatc taaatcacaa gctaagaatt tgataatgac tgaatcagct
     1561 ttctttgcat cagcttcaag aaaagattgt ttcaatgatt tagatgtaga gaagtatgag
     1621 attattgcca cactagactt aaaaacatca aatatatgta gagagttaga tggaaaagta
     1681 tttgatatga aggattatca agttggagtt acagctccac catttcattg tcgttgtagg
     1741 acaacaacag ctccttggtt tgaggatgaa gaaggttaca gagcagcaaa aggagaagat
     1801 ggaaaaacat attatgtacc atctagtatg aagtataatg agtggtatga gaagtatgtt
     1861 aaacataata gtatcttaga aataaaaaat agtgctataa tagatagcat aaaagaagat
     1921 attaaaaatg gtaaatataa tttaaatatt catgatggga aacaaggaaa acatttaaaa
     1981 gagcataata attatataga aggaagaagc tatttaacta taacaaaaga agaagctcaa
     2041 gaacttgtga ataagcatgc tggcaatgga atcataaaat ttaatcgaag tggagaatgg
     2101 gataaaaaag aacttataga agttgataag aatatagggg tcaatgttaa taatattacg
     2161 ggtgaaaaaa ctcttacaaa taagtttaag atacattatt ccaaaactgg aacacatata
     2221 gtaccagctt tataaggagg gaaaataaaa tgaaattatg ggaatatgta gggaaaaatg
     2281 ttcaaataac ttgtgtagat aagcaaataa taagaggaaa gtgtgatgga tatacacaag
     2341 ctttagataa tgaaccagaa atagcaagta tatcaatagc tagggatggt tatggaattg
     2401 aagtttatga aaatgaaata gaatctatcg aaaatataaa taaggaatga ctaagcatgt
     2461 acttaaaaat aagtagatgc ttttattttg taaaaaatga aaggagaaat ttaaaatgga
     2521 ttggttaaaa gaattgctag aaggaataaa aatagaggat aacaagattg atgtagcttc
     2581 tttacaaaag tctatagaaa agaaaataaa agagactaca attactcaag aagattatac
     2641 aaatcttgaa acacagctta atacagctaa tgaaactatt aaaaagtttg aaggaggtat
     2701 gacaaaagaa gatgtagaga atctaaaaac aacttatgaa actgataaga aaactttgga
     2761 agaaacctac aaaaaagaaa ttgaagaaaa ggactttaat tactggttaa atgatgcttt
     2821 taagtctatt aaatgtaggg atgaaatagc gttaaaagct catttagata tagaagcact
     2881 aagaaatagt aaagatagac aaaaagcttt tgaagagcaa ataaaccctt tgaaacagga
     2941 taaagattat ttgtttaatg caacactaga aggtgaagag cctaaaatag atactataac
     3001 accagggcaa gagcctaaga taaatgattt tggttttaat tttactgggg taagacctca
     3061 tgaaaataat aataaatagg aggaaataaa atggcagcac taaattatgc aaaagaatat
     3121 tcaaatgttt tagcacaagc atatccttat actttaaact tcggggattt gtatgcaaca
     3181 ccaaataatg gaagatatag atggactggt tctaaaacaa tagaaatacc aactatatct
     3241 acaactggaa gagtagattc aaacagagat acaatagcag tagctcaaag aaactatgat
     3301 aatgcttggg aacctaaggt attaactaat caaaggaaat ggtcaacatt ggttcatcca
     3361 gcagatataa accaaactaa ttatgtggct tcaataggca atataacaaa agtatataat
     3421 gaggaacaaa agtttccaga gatggatgct tactgtatat ctaaaatata tgctgattgg
     3481 accgcattag gtaatacagc agatacaact gttcttacaa caacaaacgt attagaagta
     3541 tttgataagt taatggaaaa aatgacagaa gctagagtac ctgaaaatgg aagaatattg
     3601 tatgttactc cagtagtaaa tacacttatc aaaaatgcaa aagagataca aagaacagta
     3661 aatataaagg atgcaggaac ttctcttaat cgtcaaacaa ctgatattga cacagttaaa
     3721 ataattaaag taccatctaa tctaatgaaa actgcatatg attttacaac tggatggaaa
     3781 gtaggagcag gagctaaaca aatctttatg tccttagttc acccaagtgc aataattaca
     3841 cctgtttctt atcagttctc taagttagac gaaccaacag cagttacaga gggaaaatac
     3901 ttctactttg aagaaagttt tgaggatgta tttatattaa ataaaaaagc tgatgcaata
     3961 caatttgttg ttgaaggagc tggagcataa tggcacaagt aaggaaatta aatagaatat
     4021 taaccataga agagtgtaaa atagatgatt tcttagagat gggatatgat ttgatagatg
     4081 aaactggtaa ggtagtaagg tatggcaagt cattaaatgt aaaagattta atagctgaaa
     4141 ataatatttt aaggtcaaaa gttgagtctt tagaagaaga aaataagcag cttaaagaga
     4201 aaaataaact tactaaaaag taggtgaaaa ttatgggaaa taatataatt gatgatatag
     4261 aaaaaagact tgaaagtttt ggatatatat taaaagatgg ggataagtgg ttaatagatt
     4321 ttgtaagaga aaaaatagaa aatattatta aactagattg taatataaaa actatgccaa
     4381 ttgaattgaa agaaattgaa gctgatatga tagttggaga gttcttattt accaagaaaa
     4441 atatggggca attagatata gaaagcatta actttgaagc tgtagaaaag tctatatcag
     4501 aaggtgatac aaaggtagat ttcgctatag gaagtggctc tcaaacacca gaacaacgct
     4561 ttgatagctt aatagcttat cttactgctt atggtaagaa taagatatta acctttaggt
     4621 gcttaagatg gtaagtaaaa ctagaaaagc aatagaaatg ttatatagat ataaatgtac
     4681 tatagttgag tatcagccaa tcaaagaccc tgtaacaaaa cgaactaaca ataaagaagt
     4741 gattgtatta gaaaatcaac catgcaagct ttcatataaa aatatagttt ctgctacaga
     4801 aggaaaatta gctaagctag agcaaactat taaactcttt atatctccag atatagaaat
     4861 taaagcaggt tcaaaactta ttataaatga taaagagtat gtaagaagtg gagaatcagc
     4921 tatatatcca aatcatcaag aaataatact tgagttattt aaggataaag cataatggct
     4981 agatggggca gtgttgattt tagagagttt aaaagagttt gtaaaaagat ggagaagctt
     5041 acaaagattg atttagataa gttttgcaaa gatgcagcaa gagaattagc agcaagactc
     5101 ttaggaaaag taattagaag gacaccagtt gatacaggat tcttaagaca aggatggaat
     5161 ggagtggctt atgctaggtc gcttcctgtg tataaacaag gaaataatta tattatagag
     5221 gttgttaatc cgactgaata tgcaagttat gtaaatttcg ggcatagaac taaagacgga
     5281 aaaggttggg ttaaaggaca acatttctta acaatttcag agatggaact acaaagccaa
     5341 gttgataaga ttatagagaa aaagttatta atattactta aaggagtatt tgatgcttaa
     5401 taatattata gatggaatat cagtaaagtt agataaatca tttggagaaa aatatacaat
     5461 ttatagtgag gatgtagagc aaggtattaa tgaaccttgt ttttttattg ttcctttaaa
     5521 tccaagcaag acaccatatc caagcgggag agaattaaag aaaaattctt ttgatgtaca
     5581 ttatttccct cgttcagaag ctaagaattt tgaaataaat gagatagctg agatgttact
     5641 ggaagaatta gagtatatag aaatcaatgg agatttagtt agaggcacaa atatgaattt
     5701 tgaaattata gacaatgttc ttcacttctt tgttgattat aactacttca ctataaagag
     5761 taataatgca gataaaatga atacagtaga gttattcggt ggtttgaaga gaggtgataa
     5821 ttttgagtaa gacattaagc aaaggaaccg attacaagtt tactaaggag cagatagtta
     5881 attctaagaa gtatgtaaat agaaaagact tattaaatgc aattttaaaa gaaaatgagt
     5941 tatattcctt ctcagaagta gaggatagaa taaataaatt catgaaagga gtgagttaga
     6001 tggctttagg tggaggaaca tttgtaacac agaataaagt attaccaggt agctatataa
     6061 actttgtaag tgccacaagg gcaaccagtt cacttagcga tagaggtatt gttgcaatgc
     6121 ctttagaact tgattgggga attgatgaag aagtatttca agtgactagt gatgattttg
     6181 agaagtattc aactaagtat tttggatatg attatacaca tgagaagttg aaaggtttga
     6241 gagatttatt taaaaatata aggctaggat atttctataa attaaataaa ggtgttaaag
     6301 ctagttgtag tattgctaca gctagatgca gtggtataag aggtaatgac ttaaaagtta
     6361 tagtaacaac aaatatagat gataatgcta aatttgatgt tgtaacactt ttagataata
     6421 agaaagttga tactcaaata gctaaagtta ttacagaact gcaagataat gattatgtca
     6481 cttggaagaa ggaagcaaca ttagaggcta cagcaggatt aacttttact aatggaacta
     6541 atggtgaagc tgtgacagga acagagtatc aagcttttct tgataagata gaaagttact
     6601 catttaatgc actaggatgt ttggctacaa cagcagagat taaaagtttg tttgtagagt
     6661 ttactaagag aatgagagac aaagttgggg ctaagtttca aactgtatta tataaaaaga
     6721 atgatgcaga ttatgaaggt gtagtatctg ttgaaaataa gattaaagat actggattat
     6781 tagaatctag cttaatttac tggactactg gagctatagc aggatgcgat ataaataaat
     6841 ctaatactaa caagcgatat gatggtgaat ttgatgtaga tgttaattac actcaaatac
     6901 atttagaaga agctttaaaa actggtaagt ttatatttca taaggttgga gatgaagttc
     6961 atgtattaga ggatataaat acttttgtta gttttacaga tgaaaagaat gacgactttt
     7021 caagtaatca aagtgttaga gtacttgacc agattgctaa tgatatagcg actttattta
     7081 atacaaagta cttgggtgaa gtaccaaatg ataaatctgg tcgtatctcg ttttggaatg
     7141 atgtagttaa gcatcatgaa caactgcaaa atatgagagc aatagaagat ttcaaagctg
     7201 atgatgtttc tgtagaacct ggaagcgaca agaagactgt tgtagtaagt gatgctgtaa
     7261 aagttattag tgctatgagt aagctttata tgactgtttc agttagttaa caataagaaa
     7321 ggagaataat aatggcacaa caaataaaag caagagatac aataagtgca tctaaggcag
     7381 agtgttttgt aactataaaa ggtaaaagat ataattttat gcaagctatt aacttagaag
     7441 ctaaaatgga aaagaataag agtgagatac ctatattagg tagtactaca aaaggaaata
     7501 aatcaacagg aagtaaatat tcaggaaatg caacatttta ttataatacc tctatattta
     7561 gagaattgtt gtatgagtat aaagaaactg gtgaggatat ttacttcgat atacaaatta
     7621 ccaatgaaga cccaacaagt tcagtgggtc gtcaaactat aatactggaa gattgcaata
     7681 tggactcagg cataattgct aaatttgatg ctgatgggga gtatttagat gaagatatgg
     7741 attttacttt tgagaattgg aaattagttg agaaatttaa tatagcaaat ggtatggagt
     7801 aaaatacaca tttatgattt atgtatgtgt attttttatt tataagaata ggagatgatt
     7861 aaaattaagg ataaatatga gataaaagat tcaatttctt ttgattatag caataaaaga
     7921 cctttggaag aacgtgtcag cgagatgtat aaaaaggcag gaaaatatct tatagatatt
     7981 tcagataagt tagcaacaga tacaattgat ggttcgtcat taaagccaat aatcataaaa
     8041 tttgaaataa atgaagctgg tgttgcaaca atagaaaaac aaacaaaata tttggtgatg
     8101 gaggtagaat aagaatatgg gagatttaaa cgctttttta agtcaaaatg caataaaagt
     8161 agagaataga aagtatgtgg caagtgaaag gtttatagga gaagatggaa aagcaatcga
     8221 atgggaactt aaagcaatag attcagatag agatagacaa ttaagaaaag attcaactat
     8281 aagagtacct gtactaaata aaaaagggaa agcaacaggg caatacacta gtgaaacaga
     8341 ttttaatact tatactttga aactgtgtgt agaaactgta gtatttccag atttacatga
     8401 tgcagaactt caaaatagct atggtgtaat gggagcagag gaactattaa caacaatgtt
     8461 aactcctggt gaatacacag acctttcaag tgaggtagga gaagtgaatg gttttgatag
     8521 gacttttgaa gataaagtag aagaagcaaa aaactaattg aaggaggcga ttatgatgct
     8581 agtgtagctc attattgcct tcataaattc aaatggaaac cacatgaata tacagattta
     8641 ccagacttcg agagggcatt tgttgctgct tctatagata ttaaagtaga agaagaaata
     8701 aaagaagaaa aaaagattgc taaagaagct agaagaagta gaagaagata aaatataggt
     8761 aaaatatgta agaattatat gttataataa ttgtagcaag aagatgtaat ctacaattta
     8821 tagagtggag ttcatactgg gataaaacct acttcctaat gaaaggaggt gggaagtatg
     8881 gacaattttt tacttagtat attagctagc ttgatagcta gtttaattgg atatatcgtt
     8941 tgtagatgta tcaaaaacgt aaaaagccac tctactcgtg gcaagagtaa aagtggctgg
     9001 gaacttggtt ttaaaataaa gttccgcaaa tttaaataat ttatattttt taaaattatg
     9061 aacttcactc taccgctaaa tagattgtag ttcttcttgc ctttattata ccacaaatta
     9121 gaaaaaatat tgtttatata aaataaaaaa taaaaatttt tattaaaaaa ttgaaaactt
     9181 gattataaag caattaattt ataaaatata tataaataag taggtattta tttacttgaa
     9241 tttcattgtt tatataaaaa aaatggaaaa atatgtaata attatatgtt ataataattg
     9301 tagcaaggat aataatcgaa agtgcgaagg gtgattattt tcatattaaa cgccaaattc
     9361 caaataagga aggaggtgaa attatatgat aggtttttta ttaagcatac tagctggtgt
     9421 tatatcagct tatatttatg acaaaataaa aaatcaccca gacgccaata agggtgattt
     9481 aaaaaaataa ttctttaaat caattttgat ggaaatagct actcttgtat aaagtaaatt
     9541 atttccttgc ttttattata cc
//
LOCUS       DAEPIN010000104         8959 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.106, whole genome shotgun sequence.
ACCESSION   DAEPIN010000104 DAEPIN010000000
VERSION     DAEPIN010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8959
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.106"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            complement(58..279)
                     /locus_tag="KR250_003370"
     CDS             complement(58..279)
                     /locus_tag="KR250_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069258.1"
                     /translation="MNVNNHKCPECGEHSKKFEIVEENEDCVIFECYTCGTISILKYC
                     ENCKEFVFVNIPNNVETYEYCCGKCNNKI"
     gene            complement(781..1761)
                     /locus_tag="KR250_003371"
     CDS             complement(781..1761)
                     /locus_tag="KR250_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069259.1"
                     /translation="MGDTSRFYESVKNKYPKLKDGTRVHLWPKENIIIGPIYDEEDGY
                     KKSIGNVDSLSWKQIVILAECDGKNTTDDIINILSNRYRNIHNMKEKVMEFFMFYENV
                     YLTFEDDIQTLSSVFEITGNKSYITPLYFTIEIDGDNNTNDYFSEITSLLNCMYEKGC
                     RFIEVIGEDILKNKSMREIFQYMLDHFDLIVVTKDSFTIDRSLIKELDNYRHKVIWKV
                     YSKNDDTRIKLKEDIKITSLIKRGHTVVRGDREKAIKIEDMRFKDTKERFSKYGAEWS
                     HLYISSDGSVKSYSFQKDKRIYLGNIFNNSVEEIFLEMQNKIDKLEIVYQ"
     gene            complement(1925..2797)
                     /locus_tag="KR250_003372"
     CDS             complement(1925..2797)
                     /locus_tag="KR250_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBH0069260.1"
                     /translation="MIIKVSMTVLVLILLYILYKYETQHIETTEYTIFNKKIPKEFDN
                     FKIVQVSDLHNKVFDKNNKVLIDKIESLNPDVIFITGDLVDGENKNFQVALDLIDNLV
                     EKYEVYHIIGNHEQKSLIKKYKHLYKDYFKELYSKKIINMENESVRIYRGNSHLNLYG
                     LIIPLECYPYLFANNKKLELSDNFVEDNLGKVNENEYNILLAHTPFFFEKYAKWGADL
                     VLAGHVHGGIIRIPFVGGVLSPNREFFPKYDWGKYEKDNSTMILNKGLGGSKVLIRLN
                     CKPEIVKITFKYEK"
     gene            complement(2961..3512)
                     /locus_tag="KR250_003373"
     CDS             complement(2961..3512)
                     /locus_tag="KR250_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH0069261.1"
                     /translation="MDNKFPNVGENLRLLRQEMGISLDKASKMTGVSKAMLGQIERGE
                     SSPTVSTLWKISSGFKINFTTLLNENTNTYEVIKKEEVEPIVEQKGNMKLYPIYPFSP
                     QRRFELFIIELEENCTHVSSTHSHVLEEYVLVIEGKLDLNVDGKTYILESGHSIRFDG
                     TLEHIYKNLNKGKTIFHNIEVYR"
     gene            complement(3532..4434)
                     /locus_tag="KR250_003374"
     CDS             complement(3532..4434)
                     /locus_tag="KR250_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HBH0069262.1"
                     /translation="MSKKGRVVKVQHFSVNDGDGIRTTIFLEGCKLKCKWCSNPDSWS
                     NIVKLGVMKDKCVSCNRCIDVCPQNISSLFDRAQINNKCDLCGECIKVCLKDAICIMT
                     EEMSVEEIVEEVEKDFIFFFESNGGITFSGGEPTLQIDFLRELVDIFYDKGINIAIET
                     CGYFDWNKVNDVFEKIDHIFVDIKSMDDNIHKEYTGVSNKIILDNICRLSKLNKSMVI
                     RVPIIYGVNDSEENIRNTALFVKQNVPGGKMELLPYHKFGIDKYKALGLEDYIYEFDD
                     ICNNHMLKLKEIVELTGVKIIEYK"
     gene            complement(4449..6821)
                     /locus_tag="KR250_003375"
     CDS             complement(4449..6821)
                     /locus_tag="KR250_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase/glycerol dehydratase
                     family glycyl radical enzyme"
                     /protein_id="HBH0069263.1"
                     /translation="MSQTNRIEAFRQEYINSKPMICCERARIFTESHKKTEGEAICIR
                     RAKAFLETCKELPIKIFENELIVGTAGKFRRTGILTPEFSWQWVDKEMDTFDKRTQDP
                     YVISKEQIEFIRKEIFPYWKGKSLEEVFLARIPEDTAKILVDTGIIDNDSKWRQAVGE
                     VTPDYQDILFVKGYKGIKEDADKKIKELDISVSENIEKIDFYKSVSIVAQGIMTLAQR
                     YSNLAKEMSKQETDEKRKLELIKISEICMNVPANPPTNFYEAIQFVWFVQLGGILSEN
                     PLALNLGRFDQYMYPYYENDAREGKITESEAQELIEALWIKLSEWVWTISANTANYFA
                     GYNQFQNLTVGGKKRNGTDGTNDISYMCLKATESVKTHQPGLSVRVSQGAPDNFVMAV
                     AKLVKQGTGFPAIHSDSAGAQMLLQDGYDAEDARDWSNCGCVVPHFRKTGQWTSAVNI
                     NFAAALEYAMNEGKSRLTGEKMGLDTKNITEFTSFEELKDEFLKQLAYLVKSSVIGTT
                     VAQQIHKEMVPRPFLSTCVDGCLDKGVDLSKGGAKYNIGPVLTGIGLGVVSNSLAAIK
                     KLVFEDKVTTLEELTKALNNDWEGYEELRKLALDVPKYGNDNDYVDSLAIEVSDFYYT
                     ETRKYKDIFGSKFNSAFMGISNYVPTGKIVGATPCGRKATKPLTEGVSPFVGTDTTSP
                     LAAMKSASKINHDVHTGGTLLNLRLNQDLVETERGLRNLTSMIKSYFALGGFHVQFNT
                     ISNDTLLKAQENPEEYKDLLVRVAGYSTQFVNLSREMQDAIIARNSHSNF"
     gene            complement(7042..7731)
                     /locus_tag="KR250_003376"
     CDS             complement(7042..7731)
                     /locus_tag="KR250_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069264.1"
                     /translation="MKVKRNINRVAIIVALNLSLALMVGCMSPVYDKNLSSSEQISKD
                     SLKEQVEDKKDESIELFNNYSQKINDAKGREEVKKDLSQRFSKAIIDSGYSITDSKSD
                     GSFVVSINGAEKPPEKNIRSLFYSMSEDDKEGKVTIDILCAKEYPEDDKLSESDKYIK
                     FIYNLFKSLTNTNLTEKEIFSMVEEDFNKGVGVVELPYMKDIHVEVNKVKQSTKVLKL
                     SLVYKFDISNH"
     gene            complement(7972..>8959)
                     /locus_tag="KR250_003377"
     CDS             complement(7972..>8959)
                     /locus_tag="KR250_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBH0069265.1"
                     /translation="PHLESIKIQIYSNLIIILIYLIFSFFRKSIKLISQDKRNYVYLL
                     FALLVNTMNMLVNYVLKKLERFGSLHSEGYYFDNFVNPKLVGASSIFLILLFKEIIKE
                     NRLKSQAELIKNKLDMQYAHYLSIQESHMRVKKLYHDINNHIYCIDNLRNNSKEINEY
                     VNNLKDEIKTFKYIYNTGNMILDIIINEKSEICLKKGIKFTCSINFSKVNFVKPIDVS
                     SIFSNILDNAIEACDKIVDENVNKYIRIKGTMTRSFFVLKCENSKLNQLTFNKNILLT
                     NKMDKFVHGIGIQSIKSSLQKYNGELLFENSIDKFILNIYIPLDQDTDSWGD"
BASE COUNT         2902 a         1571 c          846 g         3640 t
ORIGIN      
        1 gaattttata tgagattggt ataaatgata tatgaaatta catatgttta tattatatta
       61 aattttatta ttacattttc cacaacaata ctcatatgtc tccacattat ttggaatatt
      121 tacaaataca aattctttac agttctcaca atactttaat atagatatag ttccacaagt
      181 gtaacattca aatattacac aatcctcatt ttcctctact atttcaaact ttttactatg
      241 ttcaccacac tctgggcact tgtgattatt tacattcata aatactcctc cttattagta
      301 tagttacatg tcaaaaactt aattaatgta ttctatatta cttattatat attctataat
      361 aaatcatatt ctaatataat aaaacaaatt aaataaaaaa aaaaccataa attttaaatt
      421 tatggattaa ttcttgtaat gataactcta tttgaggatg tgagggaggg ttatcattac
      481 aatagggtat tatattatgg tttttaagct agtctttcta cacctcatat gatacatatg
      541 ctatatgaat tttatatgat ttttacataa atattttatg aaatttaaat tttttagtca
      601 ttttgaaatt tgtaaaaagt tctctaaaaa tccacagcat cataaacagc caaaatttaa
      661 gtaaaacatc ataaaaaaat ttgtccaata attttaacaa gtaatctcac ctcattataa
      721 ttattgacac aaacttaaat aaacaatcta taaatcttct ataaatctta aactttcccc
      781 ctattgatac acaatttcta gtttgtcaat tttattttgc atttctaaga aaatttcttc
      841 aacagaattg ttaaatatat tgcctaaata gattctttta tctttttgaa atgaataaga
      901 ttttacacta ccatcagaag aaatatataa atgactccac tctgctccat attttgaaaa
      961 cctctccttt gtatctttaa acctcatatc ttctattttt attgcttttt ctctgtctcc
     1021 tcttactaca gtgtgacccc tttttattaa actggttatt tttatatctt cttttaattt
     1081 aatacgtgta tcatcatttt ttgaatatac tttccatatc actttatgac gataattatc
     1141 taattccttt attaagcttc tatcaatagt aaaactatct ttagttacca caattaaatc
     1201 aaaatggtct aacatatatt gaaatatttc tctcatactt ttatttttta aaatatcctc
     1261 accaataacc tctataaatc tacagccttt ctcatacatg cagttcaata aacttgtgat
     1321 ttcagaaaaa taatcatttg tattattatc tccatctatt tctatagtaa agtacaaagg
     1381 agtaatgtat gatttatttc ctgttatctc aaatacactt gatagtgtct gtatatcatc
     1441 ttcaaacgtt aagtacacat tctcataaaa cataaaaaat tccataactt tttctttcat
     1501 attatggata tttctatatc tattggataa tatatttatt atatcgtcag tagtattttt
     1561 cccatcacat tcagccagta taacaatttg cttccaactt aagctatcca catttccgat
     1621 acttttttta tatccatctt cttcgtcata aataggacca attataatgt tttcttttgg
     1681 ccacagatga accctagttc catcttttaa tttcggatat ttatttttta cagactcata
     1741 aaatctactt gtatcaccca tgattcttta ctacccccca tagtttaaat attctatgct
     1801 aaaaatagca ctttattttt attaattcaa caattattca aaattggtca ttatatatgt
     1861 ttaaatggat ttaacatcaa ccttatttat atgttattga tgtttatata tgttatataa
     1921 aatcttattt ctcatactta aatgtaatct taactatctc tggtttacaa ttaagcctaa
     1981 ttaaaacttt tgagcctcct aagcctttat ttaaaatcat agtgctatta tctttttcat
     2041 attttcccca gtcatactta ggaaagaact ctctatttgg agataataca cctccaacaa
     2101 aaggtattct tattatacct ccatgaacat gtcctgctaa aaccaaatct gctccccatt
     2161 ttgcatattt ttcaaagaaa aatggtgtat gtgctaaaag aatgttatat tcattttcat
     2221 taactttacc tagattgtct tctacaaaat tgtcacttag ttctaatttt ttgttattag
     2281 caaataaata tggatagcat tccaatggta taattaaacc atataaatta agatggctat
     2341 tacctctata tattcttaca gattcatttt ccatatttat tattttcttt gaatatagtt
     2401 ccttaaaata atccttatat agatgtttat acttttttat taaagatttt tgttcatgat
     2461 ttccaattat atgatatact tcatattttt caaccaaatt atctattaaa tctaatgcaa
     2521 cttgaaaatt tttattttct ccatctacta aatctcctgt tataaatata acatctgggt
     2581 ttaatgattc aattttatct atcaatacct tattattttt atcaaacacc ttattatgta
     2641 aatcactcac ttgaactatt ttaaagttat caaattcctt tggaattttt ttgttgaaaa
     2701 tagtatattc tgtcgtttct atatgctgag tttcatactt atacagaata tacaataata
     2761 ttaatactaa tactgtcatt gaaaccttta taatcatatt ctcttcctct ttattctatt
     2821 ttgttaatat tattataatg cttttatgtt ttttttaaaa caataacatc atcacataca
     2881 tataaatctt ttcgacatta taaaaataag ttatcctttt ctaaaatttt catttatttt
     2941 agaaaaggat aactataaaa ttatctataa acctctatat tgtgaaatat agtttttcct
     3001 ttatttaaat ttttatatat atgctctaaa gttccatcaa acctaataga atgtccagac
     3061 tctaaaatat aagttttccc atccacatta aggtcaagtt taccttctat tacgagcaca
     3121 tattcttcta aaacatgact atgtgtagat gaaacatgag tacagttttc ctcaagctca
     3181 attataaaaa gttcaaacct tctttgaggg ctaaaagggt atattggata taacttcatg
     3241 ttcccttttt gctcaacaat aggctctact tcttctttct ttataacctc ataggtattt
     3301 gtattttcat ttaatagtgt agtaaaattt attttaaatc cagatgaaat tttccataaa
     3361 gtagatactg tcggacttga ttcacctctc tcaatctgtc caagcattgc tttactgaca
     3421 cctgtcatct ttgaagcttt gtctaaactt atgcccattt cttgtctcaa taaccttaaa
     3481 ttttctccca cattaggaaa tttattatcc atgttaaaat cctcctgata gctatttgta
     3541 ttctatgatt tttactcctg taagttctac tatctctttt aattttaaca tatgattgtt
     3601 acaaatatca tcaaattcat atatataatc ttcaagccct aatgccttat atttatcgat
     3661 tccaaattta tgatatggaa gtaactccat cttgcctcct ggaacatttt gtttgacaaa
     3721 taatgcagta tttcttatat tttcttcact atcatttact ccataaataa ttggcactct
     3781 tataaccata gatttattca actttgaaag cctacatata ttatctaata taattttatt
     3841 acttactcct gtatattctt tatgaatatt atcatccata gattttatat ctacaaatat
     3901 gtggtctatt ttctcaaata catcatttac cttattccaa tcaaaatatc cacaagtttc
     3961 aattgcaata tttatgcctt tatcataaaa aatatcaact aattctctta aaaaatcaat
     4021 ctgaagtgta ggttcacctc cagaaaaagt tatccctcca ttagattcaa agaaaaatat
     4081 aaaatctttt tcaacttcct ctactatttc ttcaacactc atttcttcgg tcattataca
     4141 aatggcatct ttaagacata ccttgataca ctctccacat aaatcacatt tattatttat
     4201 ttgcgctcta tcaaatagtg aacttatatt ttgaggacaa acatctatac atctattaca
     4261 ggatacacat ttatctttca taacacctaa ttttactatg ttactccagg aatctggatt
     4321 agaacaccac ttacatttta atttacatcc ttctaaaaat atagtagtcc ttattccatc
     4381 tccatcattt actgaaaaat gttgtacttt tacaactcta ccttttttac tcataatcta
     4441 cctcataatt aaaaattgct atgtgaattt ctagctatta tagcatcttg catttctctt
     4501 gataaattaa caaattgagt actatatcca gcaaccctca ctaataaatc tttatattct
     4561 tctggatttt cttgagcttt taatagagta tcatttgata tcgtattaaa ttgtacatgg
     4621 aatcctccta aagcaaaata tgatttaatc atagatgtta gatttctaag tcctctttca
     4681 gtttccacca aatcttgatt cagtcttaaa tttagaagtg ttccaccagt atgaacatca
     4741 tgatttattt ttgatgcaga tttcattgct gctagtggac ttgttgtatc agtaccaaca
     4801 aatggagaaa ctccctctgt caaaggtttt gtcgcttttc ttccacaagg agttgcacct
     4861 actatttttc cagttggtac atagtttgat atacccataa atgcactatt aaatttagag
     4921 ccaaaaatat ccttgtattt tctagtttca gtataataaa aatcagaaac ttcaattgct
     4981 aatgaatcta cataatcatt atcatttcca tacttaggaa catccaacgc aagttttctt
     5041 aattcttcat aaccttccca atcattattt agtgcttttg ttaattcttc taacgtagtc
     5101 accttatcct caaatactaa tttttttata gctgccaatg aatttgaaac tacacctaac
     5161 cctataccag ttaatacagg acctatatta tattttgctc ctcctttact taaatcaact
     5221 cccttatcca aacatccatc tacacaagta gataaaaatg gtcttggtac catttcttta
     5281 tgaatttgtt gagcaacagt agttcctata acagaacttt ttacaagata tgctaattgc
     5341 tttaaaaact catccttaag ttcttcaaaa gaagtaaatt ctgtgatatt ttttgtatct
     5401 aaccccattt tttctccagt taatctactc ttaccttcat tcatagcata ttctaaagct
     5461 gctgcaaaat tgatatttac ggctgaagtc cactgtcctg tttttctaaa atgaggaact
     5521 acacaaccac agttactcca atctctagca tcttctgcat catatccatc ttgtaataac
     5581 atttgtgccc ctgcactatc gctgtgtata gcagggaaac cagttccttg ttttaccaat
     5641 ttagcaactg ccattacgaa attatctggt gctccttgac tcactctaac acttaatcct
     5701 ggttggtgag tttttacact ttctgtagcc tttaaacaca tatatgagat atcattcgtt
     5761 ccatcagtac catttctctt tttaccacca acagtaagat tttggaattg attatatcct
     5821 gcaaagtaat ttgctgtatt tgctgatata gtccataccc attctgataa cttaatccaa
     5881 agtgcttcta tcaattcttg agcttctgat tcagttattt taccttctct agcatcattt
     5941 tcataatatg gatacatata ttggtcaaat ctacctaaat ttaatgccaa tggattttct
     6001 gatagaatac cacctaattg aacaaaccat acaaattgta ttgcttcata aaaattagtt
     6061 ggagggtttg ctggtacatt catacatatc tctgaaattt ttataagttc taactttctc
     6121 ttttcatctg tttcttgctt actcatctct tttgcaagat ttgaatatct ttgtgccaat
     6181 gtcataatac cctgtgcaac tatagaaaca gatttgtaaa aatctatctt ttcaatattt
     6241 tctgaaactg atatatctaa ttctttaatt tttttatctg catcttcctt aatcccttta
     6301 tatcctttta caaacaatat atcttgataa tctggtgtta cttcaccaac tgcctgtctc
     6361 cacttagaat cattatctat tattcctgta tctactaata tcttagctgt atcttctggg
     6421 attcttgcta agaaaacttc ttccaaagat tttcccttcc aatatggaaa tatttctttt
     6481 cttataaatt ctatttgttc ttttgatatt acatatgggt cttgagttct tttatcaaaa
     6541 gtatccattt ctttgtctac ccattgccaa gaaaactctg gtgtcaatat ccctgttctt
     6601 ctaaattttc ctgctgtacc tactattaat tcattttcaa agatttttat aggaagttct
     6661 ttacatgtct ccaaaaaagc tttagctctt cttatacaga tagcttctcc ctcagttttt
     6721 ttatgtgatt ctgtaaaaat tctagccctt tcacaacata tcataggttt agaattaata
     6781 tattcttgtc taaatgcttc tattctatta gtttgactca tttaaaatcc ccctttggat
     6841 aatatatact acatttttgt atgttataac atactttaat tatattatac tgtcctttag
     6901 cataaaatgg aagcaaaata acttattttt tttcatctac cttgaacacc tatatattaa
     6961 tacttttttt gttaaattta taaactctaa aaaaattttc tatgatatat tttctatatt
     7021 gttatagtta tttctaatat actaatgatt tgaaatatca aatttgtata ctagacttag
     7081 ttttaaaacc ttagttgatt gtttaacttt gttaacttca acatgtatat ccttcatata
     7141 aggaagctct acaacaccta ctcccttatt aaagtcttct tcaaccatag aaaaaatttc
     7201 tttctcagtt aagttcgtat tagtcaatga cttaaataaa ttgtatataa atttaatata
     7261 tttatcactc tcagataact tatcatcttc tggatactct ttagcacaga gaatatctat
     7321 tgttactttt ccttctttat cgtcttcaga catagaatag aataaacttc gaatattttt
     7381 ttcaggtggt ttttcagcac catttataga tactacaaag ctaccatctg attttgagtc
     7441 agtaatacta taaccactgt ctattatagc tttcgaaaat ctttgagata aatctttttt
     7501 tacttcttct ctacctttcg catcattaat cttttgtgag taattattaa aaagctcgat
     7561 agattcatct tttttatctt caacttgttc tttcaacgaa tctttactaa tttgttctga
     7621 gctacttaag ttcttatcat atacaggaga catacaccct accatcaatg ctaagctaag
     7681 atttaaagcc acaatgattg ccactctatt tatattcctt tttactttca tacacaatcc
     7741 tcccatcaca ctaaccgctc taaagtatta cttattgcac tagaatatgt ttatattttc
     7801 tttatattaa agatttatta ttcaatacat atcaccttcc tatccaattc ttataactta
     7861 ctattagtaa aattataagc tccttaatac agaaataagg atgttatctt ttgttattaa
     7921 aagataacat ccttattttt ttgtatcaat acatatgtcc caactggtaa cttaatcgcc
     7981 ccaactgtca gtatcttggt ccaatggtat atatatattt agtataaatt tatcgatact
     8041 attttcaaat aataattctc cattatattt ttgtaaagaa gatttaatac tctgaatacc
     8101 tattccatgt acaaacttat ccattttgtt tgttaacaat atatttttat taaaagtaag
     8161 ctgatttagt ttactatttt cacatttaag tacaaaaaat gaccttgtca tagttccttt
     8221 aattctaata tatttattaa cattttcatc cactatttta tcacatgctt ctattgcatt
     8281 atccaaaata ttagaaaata tgcttgacac atctataggt tttacaaaat ttacttttga
     8341 aaaatttata gagcaagtaa atttaattcc tttttttaaa catatttcac tcttttcgtt
     8401 gattataatg tctaatatca tatttcctgt attatagata tatttaaatg tttttatttc
     8461 atcttttaaa ttgttaacat attcattaat ttctttgcta ttgtttctaa ggttgtctat
     8521 acaataaatg tgattattta tatcgtgata tagtttctta actcgcatat gagattcttg
     8581 aatacttaaa taatgcgcat attgcatatc taacttattt tttattaatt cagcttgaga
     8641 ttttaatcta ttctctttta ttatttcttt aaacaatagt ataagaaata tactactagc
     8701 tcctaccaat tttggattaa caaaattatc aaaatagtat ccttctgaat gtaatgaccc
     8761 aaaccgttct aattttttta atacataatt tacaagcata ttcattgtat taactaataa
     8821 ggcaaataat aaatatacat aatttctctt atcttggcta attaatttta tagacttcct
     8881 aaaaaagcta aatataagat atataagaat aattattaaa ttactatata tctgaatttt
     8941 tatagattct aagtgaggt
//
LOCUS       DAEPIN010000105         8901 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.107, whole genome shotgun sequence.
ACCESSION   DAEPIN010000105 DAEPIN010000000
VERSION     DAEPIN010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8901
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.107"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            305..457
                     /locus_tag="KR250_003378"
     CDS             305..457
                     /locus_tag="KR250_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069266.1"
                     /translation="MDNFLFNVLASVTASYIVYLISKLIKKVKSHSTAKSDLKIDINF
                     KYHRKK"
     gene            651..1574
                     /locus_tag="KR250_003379"
     CDS             651..1574
                     /locus_tag="KR250_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HBH0069267.1"
                     /translation="MVGAFVLPNYAIILSEMSNLWGNFIMWILVPIITIVLLIIAVSS
                     MQYILVMIAFLLIIYSFIEKKIVMGLVSVLFFTYSIYLCATWEDKSLIADNKVETVKA
                     QRETVEREKEMERRRIQEEVDKERYIEKHGMEISENDLKVKLEALVPQEYKGKKYELK
                     VGKFKRYSMYFDLTVQNEKFSNSEECKKFVKEIANDLKKIKISKAYFKFHSKDDGGIY
                     NSVYIDYFRNIQNNVDNVENLEFNEFELKTEEEEKREQEKIEQEKNSYNNYIQNRVVD
                     PLDRIKKLKELLDSGAITQEEYNKKKKELLE"
     repeat_region   1841..2521
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=1841..1868
                     /rpt_unit_seq="gttttagattaactatatggaatgtaaa"
     gene            2713..2865
                     /locus_tag="KR250_003380"
     CDS             2713..2865
                     /locus_tag="KR250_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069268.1"
                     /translation="MKNRRKDKRGFSVLLDKEKLDKFDEVLEEKNLTKKEWLEEKIDE
                     ELEQKE"
     gene            2985..3799
                     /locus_tag="KR250_003381"
                     /pseudo
     CDS             2985..3799
                     /locus_tag="KR250_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894376.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxin Bro"
     gene            3851..4012
                     /locus_tag="KR250_003382"
     CDS             3851..4012
                     /locus_tag="KR250_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069269.1"
                     /translation="MCENLLDNMYIEKRKEEYRIKLLKIRETDIDIYNKLESIVYKLS
                     EKKLEKNNK"
     gene            complement(4341..4805)
                     /locus_tag="KR250_003383"
     CDS             complement(4341..4805)
                     /locus_tag="KR250_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069270.1"
                     /translation="MDFGEVKSELSDKDLKDNEEALNKYKKYYLKVYKYYSEMDSIKS
                     IQDIEIEQSRLKGQLQKNSNPIFALYISIMAVVFSTTINLTFDLSDITNYFIRMIMTL
                     LFIVLLIFLVNKGILPIAIPYRNNIQFYTTALDVLEDMKKKILRERKYKKRR"
     gene            complement(4941..5258)
                     /locus_tag="KR250_003384"
     CDS             complement(4941..5258)
                     /locus_tag="KR250_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069271.1"
                     /translation="MDFGNINLILIGIIVIIGTTIIYLIKPKTAFCSKKYFNKLESIY
                     GNIDKKKTVKLEVLYRYVTGLEYIAIGLFTRRLDITIIAIILVAIITTALYYLIRKKY
                     ITI"
     gene            complement(5541..5837)
                     /locus_tag="KR250_003385"
     CDS             complement(5541..5837)
                     /locus_tag="KR250_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069272.1"
                     /translation="MYFKTYILKREVNNKQLEITRSFDLDNLICTATEILKDNEIDLI
                     ITNEVNNVVWRNEKSTPPARECLVIAIEVYWWYTSIFLLYHINYVIKKSYLNRF"
     gene            complement(5856..6269)
                     /locus_tag="KR250_003386"
     CDS             complement(5856..6269)
                     /locus_tag="KR250_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069273.1"
                     /translation="MKVKSKRSFIVGIIVCMLCCASLVIYCILKDKRFLISSFLLIVI
                     AIFNFCNAFSRKGIVEELHDSADERDLYLTMKTSHILVKIMNYTLFTFTFLFIIAYSA
                     WKNQSLLVIAITLCVIEIFLFVAYLLINIFLEKKE"
     gene            complement(6253..6486)
                     /locus_tag="KR250_003387"
     CDS             complement(6253..6486)
                     /locus_tag="KR250_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0069274.1"
                     /translation="MKTKVREFRTNMGLTQQQLADLVHVSNRTIISIEKEQYSPSLML
                     AYRMAQVFGTTIEELCCLKENKEMEDKKHESKE"
     gene            6705..7079
                     /locus_tag="KR250_003388"
     CDS             6705..7079
                     /locus_tag="KR250_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBH0069275.1"
                     /translation="MKISKLPEAELKVMRYICEANKVLTSRELVAAMEEKYEWNESTT
                     FTVLKRLERREFLSTEKIGKLTHYNILVKEKKYLRFETKEFLKNIHKNSISSLMSALH
                     GEDDEVDEDKLSELEEYFKNLK"
     gene            7369..7887
                     /locus_tag="KR250_003389"
                     /pseudo
     CDS             7369..7887
                     /locus_tag="KR250_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            8319..8573
                     /locus_tag="KR250_003390"
     CDS             8319..8573
                     /locus_tag="KR250_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069276.1"
                     /translation="MENKDIKLTICIFAFVIGIIVLFCCIGLGQFNMDCIIKQNGGSI
                     ATEQYNIYLEQSISQYRNFGSILVLLGGIGILFDKSVRKY"
     gene            8609..>8901
                     /locus_tag="KR250_003391"
     CDS             8609..>8901
                     /locus_tag="KR250_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069277.1"
                     /translation="MARDVFSVKMTPLEAQKMVEKNLEADLVSVDIYNLENNKSIIVT
                     IFEKYYARSNSDAGLVVICENTTGKTLVKITSTGSAIGLFQIDWGTGNNLIKRV"
BASE COUNT         3611 a          935 c         1409 g         2946 t
ORIGIN      
        1 aaaaaaaata tttattgatt atattattta attattttat gtagatattc ttaggttggt
       61 atggaatttg tactaaccta ttaagtaatc aataaaacta aataaacata gaaagcactt
      121 acaaatatgt aggtgctttt tattgaaaaa atagaaaata aaataaaaag taagatatag
      181 gaaaaatatg taagaattat atgttataat aattgtagca agaagatgta atctacaatt
      241 tatagagtgg agttcatgta ttaaaaaatt atcctcccaa cgataagaag ggaggtgaat
      301 atacatggat aattttttat ttaatgtgtt agctagtgta acagctagtt acatagttta
      361 tttgattagc aagttaatca aaaaagtaaa aagccactct accgcaaaga gtgacttaaa
      421 gattgatata aattttaaat atcatcgtaa aaaataactt tcagttatag aacttcactc
      481 taccgcaaat agattgtagt tcttcttgtt tttattatac cacaaattag aaaaaatatt
      541 gcatataata ttttttatag tcaataaaaa tatgaaattt tttatacaca atgatgatga
      601 atctaactag atgaataaat ttaattgtaa ataagaaagt acttacttaa atggtaggtg
      661 cttttgtttt gccaaattat gctataatat tgagtgagat gagtaatttg tgggggaatt
      721 ttataatgtg gatattagta ccaataataa caatagtatt acttataata gctgtttctt
      781 ctatgcaata tattttagtt atgatagctt ttttgttgat tatatattct tttatagaaa
      841 agaaaatagt tatgggtctt gtgtcagttc tttttttcac ttattcaatt tatctttgtg
      901 caacttggga agataaatcc ttgatagcag ataataaagt tgagactgtt aaggcacaaa
      961 gagaaactgt agaaagagaa aaagaaatgg aaagaagaag aatacaagaa gaagtagaca
     1021 aagaaagata tattgaaaaa catggaatgg aaatatcaga aaatgattta aaagttaagc
     1081 tagaggcttt agtacctcaa gaatataagg ggaagaaata tgaattaaaa gtaggaaaat
     1141 ttaaaagata tagtatgtat tttgatttaa ctgtacaaaa tgaaaaattt agtaattctg
     1201 aagaatgtaa aaaatttgta aaagaaattg ctaatgatct taaaaaaatt aaaataagca
     1261 aggcgtactt taagtttcat tcaaaagatg acggcggtat atataattct gtttatattg
     1321 attattttag aaacatacaa aataatgtag ataatgttga aaatttagaa ttcaatgagt
     1381 ttgaacttaa aaccgaagaa gaagagaagc gagaacaaga gaaaatagaa caagaaaaaa
     1441 acagttataa taactacatt caaaacagag tggttgaccc actagataga ataaagaaac
     1501 taaaagagtt gctagattca ggagcaatta cacaggaaga atataataaa aagaaaaaag
     1561 aattattaga atagataata tagtaagcac ttacaagtat gtaggtgctt ttaaatttac
     1621 aaagtattcc attttaattt tatagtttag attttatgat ataataaaaa tatagaagtt
     1681 ttgcagtgtg cgatatttgt tacaaagtag ggcttaatac ttgaaatcta agatgttgag
     1741 ggtgcgtgat aagtgttatc aattgcacta ttgcccgctc actgcaattt taagagtatt
     1801 gtatatatgt aggtattgga aatgctaagt ttattttggg gttttagatt aactatatgg
     1861 aatgtaaaac tgactcggga gcatataata agtaatatca agaatagttt tagattaact
     1921 atatggaatg taaaattcgt aaaataacaa ttgtcaggaa tatgagaagt ttgttttaga
     1981 ttaactatat ggaatgtaaa attggactca aaatataagt taaaaaaatt tttaagtttt
     2041 agattaacta tatggaatgt aaaaattttt gacgctatga agaaatttac tacaaaattt
     2101 gttttagatt aactatatgg aatgtaaaaa cttttttcat cctcccaaac ctcttgttcg
     2161 ctgtcgtttt agattaacta tatggaatgt aaaaaagaaa ataagtttaa aaaaaggttt
     2221 cattattata tcgttttaga ttaactatat ggaatgtaaa accaattatg tacagtttag
     2281 ttactgtgtt aagtcttgtt ttagattaac tatatggaat gtaaaatcct tttataaata
     2341 tcttctgaca tgtgtatatg cttgttttag attaactata tggaatgtaa atcggctaca
     2401 acggtataaa ttaaatttag cagggtacat tttagattaa ctatatggaa tgtaaattgc
     2461 tgtgatttca ataataaaac aattaataat tgtgttttag attaactatg tggattcaaa
     2521 attaaataaa caaagaaagc acttacaaat atgtaggtgc ttttattctg ctcaaaattg
     2581 gtcggttggg taaaataatt agaaaaagtt agtaaaaacc tattgactgt aactcgttac
     2641 aatattgtta ttaatgtaac gagttacaga aaagaggtga ataaaatagc aactaaaagt
     2701 agggcagagt atatgaaaaa tcgtcgaaaa gataaaagag gttttagtgt acttttagac
     2761 aaagaaaagt tagataaatt tgatgaagtg ttagaggaaa agaatctaac caagaaagaa
     2821 tggctagaag aaaaaatcga cgaggaactg gaacaaaagg aataaaaaat aagggtcact
     2881 cccaccgacc aaagtttgag taacccctat gacgtatact atcgtatatc aattatagta
     2941 tatgtcattc cttaaaaaaa tcaattatta aggagtgtaa tattatgaaa aatgaattaa
     3001 tgatgtttga aggaaaagag attgaagtat ttgaatttga aggaagaatt ttattcaact
     3061 caaaagatgt agtaaactgt ttagacataa aaaatgttaa tgagaatata agacttatga
     3121 atgaaaagca agttgtaaag ttaagaaatt cggatatcag taatactgac atccgaaaac
     3181 ttaataatgc tggagaaaat tttcttactg aaagtggagt gtataaatta atctttaaat
     3241 caagaaaaga agaagctgag agattccaag gctggataag tgatgaagtt ttaccaagca
     3301 taagacaaac tggtgcatac ataaataata atgcaatacc gaaaagttga gagaaaaagc
     3361 aagtgagatt gaaaaattac agttggttta taacagtaca tctatgttaa aagaattgct
     3421 agatgatgca ggctttgaca ataaatcaaa actattaaca gctaaaacat tatataagaa
     3481 agcaggaatt gatttaccaa tagagataaa cgaagaagaa cattattttg atacaaaaca
     3541 aatagcatct aaactgaaaa tatattctaa gagtaataaa ccagctcaga tggcagtttg
     3601 tgagattatt aaaaagattg atttagaaga tggtgaagtt aaaggtgttt gggaaattaa
     3661 tggttcttgg actggtactg taaataaata tacaaagagt gtaatagata aggtgagaac
     3721 ttggatagag gaaaataata gacctaccaa gattgcaggt gagaagaaga attattatgt
     3781 attttacaaa attgagtaaa tttatcagtt gtattaaata atttagttta gttttggggg
     3841 gattaataca atgtgtgaga atttacttga taatatgtat attgaaaaga gaaaagaaga
     3901 atatagaatt aaacttttga aaataagaga aacagatata gatatatata ataagttaga
     3961 aagtatagta tataaacttt ctgagaaaaa attagagaaa aataataaat agacatataa
     4021 agcacttgga tattctactg tttcaagtgc tttgtttggt ataaaatggt ataatggaga
     4081 tagagaatta tattaaccca aaaatggaga aaatagagaa gatgatttag ttgacgcttt
     4141 atcagtagga gtacttgcag gtaagacaga atctctagta ctattagttg gaaatggaat
     4201 aagtgatact caaaaaagtt taataaataa aagagaattt agaaatataa ctcaaattgg
     4261 tggaaatgga aatgagaaag catttagtga agtagaaaac ttagttaaat agattagaag
     4321 gtagcaacta gagttggttg ttaccttctt tttttatact ttctctctct aagtattttc
     4381 tttttcatat cttctaatac atctaatgca gtagtataaa attgtatatt atttctataa
     4441 ggtatagcta ttggaagaat gcccttgtta actaaaaaaa taagaagtac aataaataaa
     4501 agtgtcataa tcattctaat aaaataatta gtgatatctg ataaatcaaa tgttaagtta
     4561 attgttgtag aaaatacaac agccataata gatatataca aagcaaaaat ggggttacta
     4621 tttttttgta actgtccttt aagcctagat tgctctattt ctatatcttg aatacttttt
     4681 atggaatcca tttcactata atacttataa acttttaaat aatatttttt atacttgttt
     4741 aaagcttctt cattatcttt aaggtctttg tcacttaact ctgatttaac ttccccaaaa
     4801 tccataaaat ccctcctaca atgttatata atatacttta atttatagca tatttaagaa
     4861 cttatatcag caatttgtcg aacgatttta gctaaaaata gagtataatg ataggcttat
     4921 tatttataag atattatttt ttatatagtt atatatttct ttctaattag ataatataat
     4981 gctgttgtta taatggctac taatattatt gctattattg taatatctaa tcttcttgta
     5041 aataagccta ttgctatata ctcaagacct gttacatagc gataaagtac ttctagttta
     5101 acagtttttt tcttatctat atttccatat attgattcta atttattaaa atatttttta
     5161 ctacaaaaag cagttttagg tttaattaaa tatattattg tagttcctat tataactatt
     5221 attccaataa gtataagatt gatatttcca aaatccatag cagatacctc ctattaaatt
     5281 tgcttttctt aatcaaagta tatcagtaac acaagtacaa agagcatcta aaatattaca
     5341 aatattaaca ggatagataa aactatagta aaaattgtaa taataaagcg aacgaaaatt
     5401 atcatactaa aacataagac atgttataat tgtattagat agaagattaa catacaccaa
     5461 agcactcctt acaataaaga gtgcttattt ttttgaaatt catcaatata taaactatca
     5521 agaaccttat tcaacacacc ttaaaatcga tttaaatagc tctttttgat aacataattt
     5581 atatgatata ataaaaaaat agaagtgtac caccaataca cttctatagc tataactaag
     5641 cattctcttg ctgggggagt gcttttttca tttctccaaa caacattatt tacttcatta
     5701 gtaattatca aatcaatctc attatctttc aaaatctctg ttgcagtaca aattagatta
     5761 tctaagtcaa aacttcgagt tatctctagt tgtttgttat ttacttctct ttttagtata
     5821 taagttttaa aatacattta tatcacccct tgtatttact cttttttctc taaaaatata
     5881 ttgataagta aatatgcaac aaataaaaaa atctcaatga cacatagtgt tatagctata
     5941 actagaagtg attgattttt ccatgcacta taagctatga tgaaaagaaa tgtaaaagta
     6001 aataatgtat aattcataat tttaactaag atatgacttg ttttcattgt taagtacaag
     6061 tctctttcat ctgcactatc gtgtaattct tcaacaatgc cttttcttga aaaagcatta
     6121 caaaaattaa agattgctat tacaattaaa agaaatgatg aaattaggaa tcttttatct
     6181 tttaaaatgc aataaataac taaactagca caacataaca tacaaactat aatacccact
     6241 ataaaacttc gtttactctt tactttcatg ttttttatcc tccatttctt tattttcttt
     6301 taaacaacat agttcttcaa tcgttgtacc aaacacttgt gccatacgat aggcaagcat
     6361 aagagaaggg ctatattgct ctttttcaat agaaataatt gttctgtttg atacatggac
     6421 taaatcagcg agttgttgct gtgttagacc catatttgtt cgaaattctc gtacttttgt
     6481 tttcattcca taacctcttt ctatatgaag ctaacttcat gtgaagttag cttcataata
     6541 tcctaaaatg agtaaaatgt caatctcatt agataagtat tataaaacct aaaagaataa
     6601 tatggtaaaa attggatgga attatttacc ttaattctct tgtttgtaaa actttattga
     6661 tatagaattt aacatataca taaatcggca aaggggcgcg aattatgaaa attagtaaat
     6721 taccagaagc ggaattaaaa gttatgagat atatttgtga agctaacaag gtattaactt
     6781 ctagggaatt agttgctgca atggaagaaa aatatgagtg gaatgaatca actacattta
     6841 cagttttgaa aagattagag cgaagggagt ttttaagtac agagaaaata ggcaaactta
     6901 cacattataa tatactagta aaagagaaaa aatatctaag gtttgaaaca aaggaatttt
     6961 tgaagaacat acataaaaat tcaatttcaa gtttaatgtc agcgttacat ggtgaagacg
     7021 acgaagtgga tgaagataaa ttaagtgaac tcgaagaata ttttaagaat ttgaaataag
     7081 aaaatgacaa attgatacat aataaataaa atgaaggtat gaaattataa ctttttctac
     7141 cttcattttg tttattattc tgaacatgtt aagaaaatga aataagtttg tattttattt
     7201 atataaattt taatagaata tgtatagtat atagtagata gaatgtataa aaataaaaac
     7261 tgttcaataa gaattaatat acattaattc agctgaatcc aatcaaaata cttaaataaa
     7321 agtaatctga aaataacata attcaaatca atttattcaa ataaacctca atttatctca
     7381 aatcttaaga agtcattaaa caaacttgct aaattacaaa gagaattatc aagaaaaaca
     7441 atcggtagtt taaatagaaa taaagcaaga ttaaaagttg caagacttca agaacatata
     7501 gctaatcaaa gaaatgattt tctacaaaaa ctatctacta aattaataaa agagaacgat
     7561 attatttgca tagaagattt acaagtaaag aatatgatta ggaatcgtaa actatctcgc
     7621 tcaatttctg atgtgtcatg gtcagagttt attcgtcaat tagaatacaa ggctaattgg
     7681 catggtagac aaattgtaaa ggtgggtaaa ttctttgcaa gttcacaaat atgcaataaa
     7741 tgtgggtata aaaacgagga agtgaaggac ttaaatataa gagaatggat ttatcctagt
     7801 tgtaatgaaa ctcacgatag agatataaat gcaagtataa atatattaaa agaaggatta
     7861 agactaataa caattcaaaa taaataacgt atgtaagaac cgtaggaact acggggatag
     7921 cctgtggaga attagtaaga catatgttag tatgcaaaat tctgtgaagc aggaaacctg
     7981 tgactttagt catgggaggt tcaggctagt attactcatt attgtattca aaagctaaag
     8041 tggaaaccta gtgaatatat gaatttagaa gttaatgaga gagcattagc agccgactca
     8101 atacttatta aaattgaaga tgaagaagaa acaatgaaag aagctgaaag agagaaaaag
     8161 agaggtagaa gaaggtagaa tttaaagtta aaatagttac attgctaata tatgaaaatg
     8221 ttacaaattt ggaaatagat ggatatatat gttgtatagt ataatgtaga taatatatca
     8281 ccaagtaaaa aacaacaaat aaatctaaat tatgaggggt ggaaaataaa gatattaaac
     8341 ttacaatatg tatatttgct tttgtaattg gaataatagt gttattttgc tgtataggtc
     8401 taggtcaatt taatatggat tgtattataa aacaaaatgg tgggtcaata gctaccgaac
     8461 aatacaacat atatttagaa caatctattt cacaatatag aaattttggt tctatactgg
     8521 ttttactagg aggaatagga atactgtttg ataaaagtgt cagaaaatac taaaaaagta
     8581 tatttttatt taggatgtga tgaatgatat ggcaagagat gtatttagtg taaagatgac
     8641 accactagaa gcacagaaaa tggtagaaaa aaatttggaa gcagatttag tttctgttga
     8701 tatttataac ctagaaaata ataagtctat aatagttaca atctttgaaa aatattatgc
     8761 aagaagcaat agtgatgcag gtttggtagt gatttgtgaa aatactacag gaaaaacact
     8821 tgttaaaatt acctcaactg gtagtgcaat tggactattt cagattgatt ggggaacagg
     8881 taataatttg attaaaagag t
//
LOCUS       DAEPIN010000106         8870 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.108, whole genome shotgun sequence.
ACCESSION   DAEPIN010000106 DAEPIN010000000
VERSION     DAEPIN010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8870
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.108"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            61..1842
                     /locus_tag="KR250_003392"
     CDS             61..1842
                     /locus_tag="KR250_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HBH0069278.1"
                     /translation="MSQYININSQLDALKLKEFEKIFYENINEDVKVVPKVTPFKGIN
                     TDLLYVKDGRILFIKFMDTTEDIFFILEEELLEVMNEEYELLKLKMGQKNRNISYNYV
                     YIMPYVEVEETYEFEEFVNNNIIDKNKLQDIMNKGSLEEYLNDENDEINLNLFLLDIC
                     SEYYIINDKLHLNEKFKKISFYNDDYKYTATMMEDVQIKDVVSIKYGNTLIEGGSGIG
                     KTAIMLSRAIKLAKVYPHHKLVIFTHTKQLCNELRERIELLYKDNNNLEVHTFSSFIF
                     KLAKKFNLIVDYNMLKNDYEKTFNNLVKQAQNIIKNKNMFKAIFIDEAESFLEYEIDF
                     IREFLYKTKFIFNVCSCNSLNISNRLNIFKKLYNGIEFDDKIILSKNYRQAKEIVDFT
                     NKFSNNSNSYINELRPNTEFSTFFYTKALRGGNKSVDIIKVSDLDDQISSVIWEIEYL
                     ISKKGLDYSEVAIVYPYNKKKLKSGKTIYFQYMLKKALEEAKIPYICAEDNLTNISKK
                     VGTTIANIYAIKNLEYKAVIVCELEMLYNQTINDIEQDYQVNDFVGDLNKVYLAMSRA
                     TDYLSIVTTFNEEASDIIRLITESKDI"
     gene            complement(1924..2454)
                     /gene="lepB"
                     /locus_tag="KR250_003393"
     CDS             complement(1924..2454)
                     /gene="lepB"
                     /locus_tag="KR250_003393"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBH0069279.1"
                     /translation="MSVKKEIFDWIKSIAMAIVLAFVILQFIRPSIVSGESMYPTLDD
                     KDYLILNRISYKVGKPEKGDIVVFKTNLVDGETGKKKDLIKRVIATEGDRIKISNSKV
                     YVNGKLLNEPYIHNNYTSGDIDTVVPKGKLFAMGDNRENSNDSRFPDVGMVDEDEVLG
                     KVMVRLLPLDNIGKVD"
     gene            complement(2494..3318)
                     /locus_tag="KR250_003394"
     CDS             complement(2494..3318)
                     /locus_tag="KR250_003394"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HBH0069280.1"
                     /translation="METFSLLEIFKAVILGIVQGITEWLPVSSTGHMILVDEFIKLNF
                     SNTFISTFLVVIQFGSILAVLVIFFRKLNPFDSAKNIKQKKETVRLWLKVIIAVIPSG
                     VIGILFEDDIDRLFFNSTVVAIALIVYGIIMIGLEKRNKKPKYKDFSQVTYKLALCIG
                     LFQCLALIPGTSRSGSTIIGAVLLGTSRYVAAEFSFFLAIPTMLGASALKLLKAGFGF
                     TGFEWLILGVGSVVAFVVSIVVIKFFMDYIKKHDFKVFGYYRIVLGIVVLAYFFLL"
     gene            3575..4483
                     /locus_tag="KR250_003395"
     CDS             3575..4483
                     /locus_tag="KR250_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBH0069281.1"
                     /translation="MYSTKILDDYEYETFNVFSTKLNPNTKHDYLSKVILFKEFLKGK
                     ELIYATKEDCKNFVDYIQTKYAKSTCEKIYSYLHSFYNFLKKEGYIDINPFRYVEKPT
                     VTRIKTKDDVLSIQEINKLIGILPKLNIRDRVIIVCLVTTGCLLNELVSLKWKDLMVD
                     ENDNSYVRLGKGRKERVVKLHPYFFKLLEDYRNYSGLPEVIIPSDDFIFTTQKSNSIT
                     DRNVRLIVKKALDLAGLSQYSARDFRHSFAAISLRLGADESDVKNQLGWSDKYYAIRY
                     KYVLNFVDSEIVDYLIEKDNLDINKK"
     gene            4627..6072
                     /locus_tag="KR250_003396"
     CDS             4627..6072
                     /locus_tag="KR250_003396"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HBH0069282.1"
                     /translation="MIDNKYTTYSNPLTDRYCSKDMSYIFSPQFKFSTWRRLWVALAE
                     GEHELGINITEEQLDELRANIDNINFDEARKIEKEVRHDVMSHVKAYGLQCEKAKGII
                     HLGATSAYVGDNTDVIQMNEALKLLRVKLVNLINNLKEFALKYKDMPALGFTHFQAAQ
                     LTTVGKRACLWAQDLILDLEDLNYRIDNMKLRGVKGTTGTQASFLSLFEGDHEKVKEL
                     DKKICKKMGYHSSYAVSGQTYTRKLDYQVISILSGIAQSMHKMTNDIRLLQSLKELEE
                     PFEKNQIGSSAMAYKRNPMRSERIASLSKYIISESISPAMVQATQWLERTLDDSANKR
                     LAIPQAFMAADAILEIGINVTDGLVVYENMINRRVNEELPFMATETILMEAVKRGGDR
                     QELHEIIREYSMKAAYRVKHEGKDNNLIELIMNDDSFKMSKEEILSIMDPKNFIGRAP
                     EQVVEFVNEVVEPAIKDYKEDLGKVDVDLRV"
     gene            6383..6835
                     /locus_tag="KR250_003397"
     CDS             6383..6835
                     /locus_tag="KR250_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBH0069283.1"
                     /translation="MNYIVKDTISFLVKTFPKIYSSLYLEDLKKFAPDYNVNKTQLRA
                     LVFIKNYGVISMTDLCSKLNIEKGSLTSMVDDLTDKKYVTRKRDLVDRRKYLIDITEE
                     GDKIATDFMDKLSDGLGEKLSKLTEEDRKKYLEAINTLQYILNKEEFR"
     gene            7247..8824
                     /locus_tag="KR250_003398"
     CDS             7247..8824
                     /locus_tag="KR250_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBH0069284.1"
                     /translation="MKQKMKGFFLQLGKSFLLPIALISAAGIFLGISSAFSNPNIVSD
                     MPFLANNFIQGILGFTKAITGVLFGNLAIFFAISISVGLAKEEKAVSAFSGFVGYLVL
                     HTSVNYMLGCRGIIAEPDMMREMGQAVTLGIQTLELGVFGGIVVGIITASLHNKYYNI
                     KLPDYLGFFGGTRFVPIITTVVFSILGIIIPYIWPYVNTIIQSVGIGIASLGYLGTFV
                     FGFLERLLIPFGLHHILNAMFRFTEVGGSLVVGGQEVFGALNIFLAQLSDPETISFST
                     EATRFLAQGKIPIMVFGLPAAAYAMYKTAKPENRSKVKGILLAGALATFVTGITEPLE
                     FSFLFVSPILFVIHAFLSGISFMMMHIFGVAIGNTQGGIIDLVVFGVMQPNTKWIYSI
                     ILGLVYAIVYYNVFKILILRFNLQTPGREEITDINFDLKPLSNKVEIAQSGETTKRAL
                     SIIKYLGGKDNIEDITNCITRLRVVVKDMSKVDENAFKKYTGAMGVIKVSDKDIQIIY
                     GPSVSQVRDEVSFAMSI"
BASE COUNT         3547 a         1043 c         1365 g         2915 t
ORIGIN      
        1 tatatataaa aatatagttc aaaatggtat aattaagtat aacagaagga ggtaatgtaa
       61 gtgagccaat acataaatat aaattctcag ttagacgccc taaaattgaa ggagttcgag
      121 aaaatatttt acgaaaatat caatgaggac gttaaagtag tgccaaaagt tactcctttt
      181 aaaggaataa ataccgattt gttgtatgtt aaagatggaa gaattttatt tataaaattt
      241 atggatacaa cagaagatat attttttatt ttagaagaag agttacttga agttatgaat
      301 gaggaatatg aacttttgaa actaaaaatg ggacaaaaga atagaaatat aagttataac
      361 tacgtttaca ttatgccata cgttgaagtt gaggaaacat atgaatttga agaatttgtt
      421 aataataata taatagataa aaataagctt caagatataa tgaataaggg ttcgctggag
      481 gaatatctta atgatgagaa tgatgaaata aacttaaatt tatttttact agacatatgt
      541 tcagaatact atataataaa tgataaactt catctaaatg aaaaatttaa aaaaatttca
      601 ttctacaatg atgactataa gtatacagca actatgatgg aagacgttca aattaaagat
      661 gttgtttcta ttaaatatgg gaatacactt attgaaggtg gttctggtat aggcaaaaca
      721 gcaattatgc tttctagggc tataaaatta gcaaaagtat atccacatca taaattagtc
      781 atatttacac atacaaaaca attgtgcaat gagcttcgag agaggataga gttattatac
      841 aaagataata acaatttaga agttcacaca tttagttctt ttatatttaa actagctaaa
      901 aaattcaatt taattgtaga ttataacatg ttaaaaaatg attacgaaaa gacctttaat
      961 aatcttgtaa aacaagcaca gaacattata aaaaataaaa atatgttcaa ggctattttt
     1021 attgatgaag cagaaagctt tttagagtat gaaatagatt ttataaggga gtttttatat
     1081 aaaacaaaat tcatatttaa tgtgtgttct tgtaactcct tgaatatctc taatagattg
     1141 aatatattta aaaaattata taatggtata gaatttgatg ataagattat cttaagtaaa
     1201 aattatagac aagctaaaga gattgtagat tttacaaata aattttcaaa taattctaac
     1261 tcatatatca acgaattaag accaaataca gaatttagta ctttctttta tactaaagca
     1321 cttagaggtg gaaataagtc tgttgatata ataaaagtaa gtgaccttga tgaccaaata
     1381 agttctgtaa tctgggaaat tgagtattta ataagtaaaa aaggtcttga ttattctgaa
     1441 gttgctatcg tttatcctta caataagaag aaattaaaaa gtggaaaaac catttacttt
     1501 caatatatgt taaaaaaagc attggaagag gcaaaaattc cttatatatg tgcagaagat
     1561 aatttgacaa atataagtaa gaaggtagga actacaatag caaatatata tgcaataaag
     1621 aatttagagt ataaagcggt aatagtatgt gaattagaga tgttatacaa tcaaactata
     1681 aatgatattg aacaagatta tcaagtaaat gactttgtag gagatttaaa taaggtttat
     1741 ttagctatga gtagagcaac agactaccta agtatagtaa caacttttaa tgaagaagca
     1801 agtgatataa taagacttat tacagaatca aaagatattt agtaaaaaaa tatttgtaat
     1861 ttaagaaaat aaaaagagac tgagatttgt aaaataaact ttaccaattc agtctcttat
     1921 tttttagtct actttcccaa tattatcaag aggtaatagt ctcaccataa ccttaccaag
     1981 aacttcatct tcatcaacca tacctacatc agggaatcta ctatcattac tattttctct
     2041 attatctccc attgcaaata gtttaccttt tggaacaaca gtatctatat ctccagaagt
     2101 atagttattg tgtatatatg gttcatttaa taattttcca tttacataca ctttagaatt
     2161 tgatattttt attctgtcac cttcagtagc tataactctt tttattaagt cttttttctt
     2221 tcctgtttct ccatcaacta aattggtttt aaaaactaca atatcgcctt tttcaggttt
     2281 accaaccttg tatgatatcc tatttaaaat cagataatct ttatcatcta aagtaggata
     2341 cattgattct ccacttacaa tagaaggtct tataaattgt agaattacaa atgcaagtac
     2401 aatagccata gctattgact taatccaatc aaatatttct tttttaacac tcataacttt
     2461 tctccttatc atttgttatt aaattagaat acattataat agaaagaaat aagcaagaac
     2521 cactataccc aagactattc tataataacc aaatactttg aagtcatgtt ttttgatata
     2581 gtccataaaa aatttaataa ctacaattga aacaacaaat gctactacag aaccaacacc
     2641 taaaataagc cattcaaatc cagtaaatcc aaatccagct tttagaagtt ttaatgcact
     2701 agctccaagc attgtaggga tggctaagaa gaatgaaaac tctgctgcta catatctaga
     2761 agtaccaagt aatactgcac ctattatagt agaaccagac ctagatgtac ctggtataag
     2821 agctagacac tggaataatc ctatacaaag agctaattta tatgtaactt gagaaaaatc
     2881 tttatactta ggttttttat ttctcttttc aagccctatc attattatac catatacaat
     2941 aagagcaata gcaactactg ttgaattaaa gaataatcta tctatatcat cttcaaacag
     3001 tattccaata actccagatg gtataactgc aattataacc ttaagccata aacggactgt
     3061 ttcttttttc tgcttaatat tctttgcgct atcaaaagga tttaattttc taaaaaatat
     3121 aacaagtaca gctaatattg aaccaaattg aattacaact aaaaatgtac ttataaatgt
     3181 atttgaaaaa ttcaatttta taaactcatc aactaatatc atatgccctg tactacttac
     3241 tggtagccat tctgttattc cctgaactat gcccaaaatg actgccttaa aaatttcaag
     3301 caaactaaat gtttccaaat caaaatacct ccaatacaaa tttatagtag catattttat
     3361 tcatagatta ataaaaaaat gccacctttt actaacttat aataccatag tactgtgtag
     3421 tgcacaatgg aagattgata ttttcataaa accttcacaa tataacaaaa taaacttaaa
     3481 agttatctat tgaataaggg gtatatttga gggggaaata tatggtatag taaaagtata
     3541 gctattttat tttatgaaaa ggaaaggagt tattatgtat agtacaaaaa tactagatga
     3601 ttatgagtat gaaactttta atgttttttc tacaaaatta aatccaaaca cgaaacacga
     3661 ttatttatca aaggttatac tgtttaaaga atttttaaaa gggaaagaat tgatttatgc
     3721 tacgaaagaa gattgtaaaa attttgtaga ctatatacag actaaatatg cgaaatcaac
     3781 ttgtgaaaag atatatagtt atttacatag tttttataac ttcttaaaaa aagaaggata
     3841 catagatata aatccattta gatatgtaga aaaacccaca gtaacaagaa taaaaacaaa
     3901 agatgatgtt ctaagtatac aagaaataaa taaacttata ggtatcctac caaaattaaa
     3961 tataagagat agagtaataa tagtctgtct agttactact gggtgtttat taaatgaatt
     4021 agttagctta aagtggaaag accttatggt agatgaaaac gataactcat atgtaaggtt
     4081 aggtaaggga agaaaagaaa gagtagttaa attacatcca tactttttta aacttttaga
     4141 agattacagg aattattctg ggcttccaga ggtaataata cctagtgatg attttatttt
     4201 tacaactcaa aagagtaatt ctataacaga tagaaatgtc agacttatag tcaaaaaagc
     4261 attagattta gctggacttt ctcaatattc agcaagagat tttagacatt catttgcagc
     4321 aataagttta aggcttgggg cagatgaaag tgatgtaaaa aatcagcttg gatggagtga
     4381 taaatactat gcaattagat ataagtatgt attaaatttt gttgatagtg aaatagtgga
     4441 ttacttaatt gaaaaagata atttggatat aaataaaaaa tagtaataaa taaaaaatac
     4501 tatatataaa tgtgaacaaa aaataaaaaa gtcttgttat agttcgtaaa attatatata
     4561 attatttatg tactaatgtg tacacagtaa tcacacgaca aaagatttaa ggaaggtgct
     4621 aatataatga tagataataa atatactact tactcaaacc cacttacaga cagatattgt
     4681 agcaaggaca tgagttatat attttctcca caatttaaat tctctacatg gagaaggtta
     4741 tgggttgcac ttgcagaagg tgaacatgag ttaggaatca atataactga agaacaatta
     4801 gatgagctaa gagctaatat agataatata aattttgatg aagctagaaa aatagaaaaa
     4861 gaagtaagac atgatgtaat gtctcacgta aaagcttatg gtttacaatg tgaaaaagcc
     4921 aaaggaataa tacatttagg agcaacatct gcatatgttg gagataatac agatgttata
     4981 caaatgaatg aagctttaaa acttctaaga gtaaagcttg taaatttaat aaacaattta
     5041 aaagaatttg cacttaagta caaagatatg ccagctcttg gatttacaca cttccaagct
     5101 gctcaactta caacagtagg aaaaagagct tgtttatggg ctcaagattt aattcttgat
     5161 ttggaagatt taaactatag aatagacaac atgaagttaa gaggagttaa aggtactact
     5221 ggtactcaag caagtttttt aagcttattt gaaggagacc acgaaaaagt aaaagagtta
     5281 gataagaaaa tttgtaaaaa gatgggatac cattcttcct atgcagtaag tggacagaca
     5341 tatactagaa agttagatta tcaagtaata agcatactat cagggatagc tcaaagtatg
     5401 cataagatga ctaatgatat aagactttta caaagtttaa aagaattaga agagccattt
     5461 gaaaaaaatc aaataggttc atcagcaatg gcttataaga ggaatccaat gagaagtgag
     5521 agaatagcat ctttatctaa gtatataatc tctgaatcta taagccctgc aatggtacaa
     5581 gccactcaat ggttagagag aacattagat gactcagcca ataagagatt ggcaatacct
     5641 caagcattta tggcagcaga tgctatactt gaaataggga taaatgtaac tgatggttta
     5701 gtagtatatg aaaatatgat aaatagacgt gtaaatgaag aacttccttt tatggctaca
     5761 gaaacaatac ttatggaagc tgtaaaaaga ggtggagata gacaggaatt acatgaaata
     5821 ataagagaat attctatgaa agctgcctat agagtaaagc atgaaggcaa ggataataac
     5881 ttaattgaac ttataatgaa tgatgattca tttaaaatga gcaaggaaga aatactatct
     5941 ataatggacc caaaaaactt tataggtaga gctccagaac aagttgtaga atttgtaaat
     6001 gaagttgtag aacctgctat aaaagattat aaggaagact taggaaaagt agatgtagac
     6061 ttaagagtat agaattgtac agataaaata taattaaaaa cggctatctt atttataaat
     6121 aagatagcca ttttaatggg ctagcgtctt ctctccaccg ctgtgaagag ttcttcttta
     6181 cttactatac catgaattta ataaaatagc tatataaata atatttaata ggaataatat
     6241 aaataaaaat aaaaaaatga taagattact aattaaattt aaacattcag caaaaactat
     6301 tgtacttgca atatttttaa tgtaaactga aataaagtta tttagataat ttaataaatt
     6361 ttaaaacaac cgaggtgatt atatgaacta tatagtaaaa gataccataa gttttttagt
     6421 aaaaacattt cctaagattt attctagcct gtacttagaa gacctaaaaa aatttgcacc
     6481 agattataat gtaaataaaa cacaattaag agcattagtt ttcataaaaa attatggagt
     6541 aataagtatg actgatttat gctctaagtt aaatatagaa aaaggcagtt taactagtat
     6601 ggttgatgat ttaactgata agaagtatgt aacaaggaaa agagatttag ttgatagaag
     6661 aaaatatcta attgacatta ctgaagaggg agataagata gcaactgatt ttatggataa
     6721 attaagtgat ggtttaggag aaaaactttc taaattaact gaagaagaca gaaaaaaata
     6781 tttagaagca ataaatactc ttcaatatat attaaacaaa gaagaattta gatagaaaaa
     6841 atataaaaat ttttgtattt aatagtacta agattaattc tttgttattc atttaactat
     6901 ataattataa gtctaaatac aatctaaaaa gtacatctta aattttcttg atgtactttt
     6961 ttataatttc ttatacattt tcgtatttgt tagtacgtac tcatatctct ttgtgtaatt
     7021 tatatttttc cattttatat tcaataattt tattttctaa gctcttttta aatttatata
     7081 tgttacaacg ttactatgtc tttgtttgaa aaaatttaca gttttgaaaa aattactatt
     7141 tatgtaaaaa tattttaaaa caaattataa agtttcaaaa acaaaataaa aatatataat
     7201 tatgacatag aaaacgtttt aaaaatttaa aaatggaggg atagaaatga aacaaaaaat
     7261 gaaagggttc tttttacagt taggaaaatc atttttattg ccaatagcat taatatctgc
     7321 agcaggtata tttttaggaa taagttcagc attttcaaat cctaatatag ttagtgatat
     7381 gcctttttta gcaaataatt ttattcaagg aatattgggt tttacaaaag ctataacagg
     7441 agtacttttt ggaaacttag caatattctt tgctatatca atatctgttg gtcttgctaa
     7501 agaagaaaaa gcagtttctg cattttctgg ttttgttggt tatttagtat tacacactag
     7561 tgtaaattat atgttagggt gtagggggat tattgctgaa cctgacatga tgagagaaat
     7621 gggtcaagct gtaactttag gcatacaaac attagaatta ggagtatttg gtggaattgt
     7681 agttggtata ataacagcat cattacataa taaatactat aatataaaac tgccagatta
     7741 tttaggattc tttggtggaa ctaggtttgt tccaataata acaacagtag tattctctat
     7801 tttggggata ataataccat atatatggcc atatgtaaat acaattattc agagtgtagg
     7861 tattggtata gctagtttag gatatttagg aacatttgta tttggatttc ttgaaagact
     7921 tttaatacca tttggattac atcatatatt aaatgctatg tttagattta ctgaagtagg
     7981 aggttcttta gttgtgggag ggcaagaagt atttggagca ttgaacatat tcttagctca
     8041 actaagtgac cctgaaacaa tcagtttttc aacagaagca actagatttt tagctcaagg
     8101 gaaaatacct ataatggtat ttggtcttcc tgctgctgca tatgccatgt ataaaactgc
     8161 aaagcctgaa aataggtcga aggttaaagg tatattacta gctggtgctt tagcaacatt
     8221 tgtaacagga attacggaac cattggaatt ttcattctta tttgtatcac caatattatt
     8281 tgttatacat gcatttttat caggtatatc atttatgatg atgcatatat ttggagtagc
     8341 aataggaaat actcaaggtg gcataataga tttagtagta tttggagtta tgcaaccaaa
     8401 tacaaagtgg atttattcta taatacttgg tttagtgtat gcaatagttt attataatgt
     8461 atttaagatt ttgatactta gatttaactt acaaactcca ggacgagaag aaataacaga
     8521 tataaatttt gatttaaaac ctttatcaaa taaagtagaa atagctcaaa gtggagaaac
     8581 tactaagaga gcattatcta taattaaata tttaggtggt aaggacaata tagaagatat
     8641 aacaaactgt ataactcgct taagagtagt agttaaagat atgagtaaag tagatgaaaa
     8701 tgcttttaaa aaatatacag gtgcaatggg agttataaaa gtatctgata aagatatcca
     8761 aataatttat gggccatctg tatctcaagt aagagacgaa gtttcttttg caatgtctat
     8821 ttaaagtaaa tattaaaagg caatactaat ttataatttg tattgtcttt
//
LOCUS       DAEPIN010000107         8853 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.109, whole genome shotgun sequence.
ACCESSION   DAEPIN010000107 DAEPIN010000000
VERSION     DAEPIN010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8853
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.109"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            230..2407
                     /locus_tag="KR250_003399"
     CDS             230..2407
                     /locus_tag="KR250_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBH0069285.1"
                     /translation="MYNKYNNLYLLLGKENVNMDSYRQLKAKIPCKETGIEIKRTLCD
                     ICSPGVHCGIDAYVKDGKVIKIEGTKEHPMNKGLLCTKGLCNREYIYREDRIKTPLKR
                     IGKRGEGKFKPISWDEAYREIAEKLNRVKSEYGAKHVAFFTGYGKWYRPFLQRFASSF
                     GSPNYGTESSSCSTSAQMAGNVATGRNFTMQDMENAKTYLGWGFNPYYSKFLNSVNLI
                     KLKEKGLKFIIVDPRITPTTQKLADLHLRIKPGTDGALALGMANLFIQKDLIDREYIE
                     KHVHGFNEYKEYVKLFDLDTVSRITGVSKDNIVKAVEMLSQGPFCINESAAPIVHHQN
                     GFQNYRAMMSLSAITGNYDRIGGNIPKQDYFAGKIGGFQTLEKEFVKEVLPKNINERI
                     GGERFPLWNEMVTQFQAMDLPRQIEEKTPYEIRALFCHGINARMFTNSGRLFKALNSV
                     DFFVNIDLFMTDTSKYADILLPACTSFEREECKSYPGGYITYTKKVIDKLYDSKSDVE
                     ILSDLANAMNIDDDLLKAGYEASIRHMFKNTCVDVDKLKESDLPLKCKDFKPYIVGSY
                     TREGYDTSTSRFELKSTIIEKYKDFGLDALPTYRGFNDNIDDEYTHLLTSGARISNAL
                     HSRLHDIKLSRILKNEASCDISSLDAKELGVISGDYIKITTKIGSLDVKVNVTDRVLY
                     KNIHMYHGYREADINSIICDNFDPYSGFPAYRSCKCKIEKMNR"
     gene            2421..2843
                     /locus_tag="KR250_003400"
     CDS             2421..2843
                     /locus_tag="KR250_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBH0069286.1"
                     /translation="MKSLFKFNKELCTACGACLLACIDQNDNNIDNGELFRHVFTIEE
                     GENIEYFSLACIHCNEPKCLGVCPKNCFKKEEGFIVLDNQNCIGCKLCEKACEYGALI
                     YGNDGKANKCNGCIERYKCGMDSPCVMVCPTGALKFNK"
     gene            3251..4054
                     /locus_tag="KR250_003401"
     CDS             3251..4054
                     /locus_tag="KR250_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBH0069287.1"
                     /translation="MKKLKLIIMFALVSALLVTGCSSGNNKNKEEQKVIRVATSGTYY
                     PFAFKDKDKLKGFEVDFWDEFAKRTNCKVEWVLTDFSGLFGLLEADKADVVSAQLTAT
                     PEREKKYAFSDAYNYSGTNIIVREDDTSIKSIDDLVGKKVGVGTGAVANEILKEKYPN
                     DEIKIVNYSSATLKGNYQDLEMGRLDAVVAQDVEALIAIKEQKLNLKMIEPPIQFGAC
                     SFALQKNEKGEKWTKEINEVIEEMYKDGTLTKISENWLGKDITKEPINE"
     gene            4047..4715
                     /locus_tag="KR250_003402"
     CDS             4047..4715
                     /locus_tag="KR250_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBH0069288.1"
                     /translation="MSNFNFEFCLSIFPFLIKALGITILISIVAFFLSILLGVILAFF
                     TQTKSKVLQYITRVYVSFFRGTPLLIQIFFFYFGFPMIFSFMKDCSPYLAIVICLGVN
                     SSAYMAEIIRASIESVDKGQQEACYAFGLSKYQSMVKIILPQAATSAVPPIVSCTLDI
                     IKGSSLGLTIGIQDLMGVAQVEAASSFKYFEIYMITILIYWAVSIILSNIQSKVEKRI
                     RRAY"
     gene            4951..5613
                     /locus_tag="KR250_003403"
     CDS             4951..5613
                     /locus_tag="KR250_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBH0069289.1"
                     /translation="MAFNMKYFLELFPTIMKSFNVTVILAISSLVFSLIIGTIVALSS
                     YYKVKVLNQICKVYVSIFRGTPLMPQLFFLYFGLAYMSDFVKNMDPVFATSIVLSLNM
                     GAYMSETIRASILSIDTGQIEAAYAMGLTNLQTMKRIVIPQAVRVALPSLFNNFIDLI
                     KGSSVAFVVGVNDIMGAAKSQGALSFKFFEVYGAVMVIYWCIITPLGWGQKKLEKKIS
                     NY"
     gene            5673..6470
                     /locus_tag="KR250_003404"
     CDS             5673..6470
                     /locus_tag="KR250_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HBH0069290.1"
                     /translation="MIKRNIIKCLVAVLSLTFVITGCSKKEEVKNEKVITVASSNSAD
                     PYSYVDNGEHKGFEVDMWKEIGKRSGYKIKMEPTGFSSIFGMLDSGKVDVAANFFGMS
                     KERLEKYDASIPYGSDSVCIAVKDGNNEINKLEDLKGKVVAVSEGSQGQQVATSVNKD
                     NLFQEKVYGDGTSGLQDLDLGRVSAWIDAELTIVGDAKKANMKVRVLDKKLSATNIAY
                     FFKKNDDKSKTIKEDVNKAIEEMLADGTVKKISEKWFGMDVSADIQK"
     gene            6588..7778
                     /locus_tag="KR250_003405"
     CDS             6588..7778
                     /locus_tag="KR250_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBH0069291.1"
                     /translation="MEVLQMIETKIKSIALKNREKIIKIRRQIHSNPELAFKEYKTSK
                     LIKEELNKLNIEYINIAGTGVLATIKGKNNGGKTILLRADMDALPIKEENDLEFKSIN
                     DNMHACGHDAHVSWLLGTAMILNDIKEELNGNVKLLFQPGEEKGGSDIVIKENVLEGV
                     DALATGHCWPTIESGKIGIARNCAMAATNTFEITIIGKGGHGAEPHNCIDPIAVGNAV
                     YSSIQQIVSRKIDPVVPVVVSVCSFNSGVSKNIIPDVCTLQGTIRAISKEQVIEVSKI
                     LEYIVKNVCKAHGADCKFEKSTGGDAVINDKNMIEIGRESACKILGAENVEIIDFPAM
                     TGEDFAIYMKEKPGLFMYIGVGDKEKNINYRLHSNKFNIDEKCLSIAPSLFSQLTVDY
                     LCQL"
     gene            7887..8636
                     /locus_tag="KR250_003406"
     CDS             7887..8636
                     /locus_tag="KR250_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBH0069292.1"
                     /translation="MIKVENLHKSFKGTEVLKGINLEISQSEVVAILGSSGTGKSTLL
                     RCLNYLVEPTKGKIMIGDVKVDVEKATKKDITNLRKHTSMVFQNYNLFKNKTALENVM
                     EALIVVHKKSKKEAREISLKLIDKVGMLDRKDFYPSKLSGGQQQRIGIARAMAVNPNV
                     ILFDEPTSALDPELVGEVLSVIKSLAKEGITMLIVTHEINFAKEVADKIIFMDNGVIA
                     EEGTPDEIFNNPKNERTAKFLNIIRKEEVFS"
BASE COUNT         3532 a          881 c         1607 g         2833 t
ORIGIN      
        1 acactctaaa tttaagacta aaattaaggc agatatgaat agcttttatc tatcacaata
       61 tatgtgtaaa taacaattta ctattagatt tttattgaat tatcttataa actatgaatg
      121 aaaaataatt atataaatag attattaata aatataaaat cttattatta aaaatattct
      181 tagaaattac actatataaa atataaagta tgatatttga ataaaaagaa tgtataataa
      241 gtataataat ttgtatttat tattgggaaa ggaaaatgtt aatatggata gttatagaca
      301 acttaaagct aaaataccct gtaaggaaac aggtatagaa ataaagagga ctttatgtga
      361 tatttgttct ccaggagtac attgtggaat tgatgcatat gttaaagatg gtaaagtaat
      421 aaaaatagaa ggtacaaaag aacatccaat gaataaaggg ttgttatgta ccaaaggtct
      481 ttgcaataga gaatatatct atagagaaga tagaataaaa acacctctaa aaagaattgg
      541 aaaaagaggt gaaggaaaat ttaaaccaat atcatgggat gaagcttata gagaaatagc
      601 agaaaaatta aatagagtaa aatctgagta tggagcaaag catgtagctt tttttacggg
      661 ttatggaaag tggtatagac cttttttaca aaggtttgca tcttcatttg gttcacctaa
      721 ttatggtaca gaatctagtt catgttcaac atcagcacag atggctggaa atgttgctac
      781 tggaagaaac tttacaatgc aagatatgga gaatgcaaaa acttatttag ggtggggatt
      841 taatccatac tactctaaat ttttaaatag tgtgaatcta ataaaattaa aagaaaaagg
      901 tctaaaattt ataattgtag accctagaat aacaccgaca actcaaaaac tagcagattt
      961 acatttaaga attaaacctg gaactgatgg agctttagct cttggaatgg cgaatctatt
     1021 tatacaaaag gatttaatag atagagaata catagaaaag catgtacatg gatttaatga
     1081 atataaagaa tatgtaaaat tatttgattt agatactgta agtagaatta caggagtttc
     1141 taaagataat atagtaaaag ctgtcgagat gttgtcacaa ggtccatttt gtataaatga
     1201 aagtgcagct ccaatagttc accatcaaaa tggatttcaa aattatagag caatgatgtc
     1261 tttatccgct ataacaggta attatgatag aataggtgga aacataccaa aacaagatta
     1321 ctttgctggt aagataggtg gattccaaac actagaaaaa gagtttgtaa aagaagttct
     1381 acctaaaaat ataaatgaaa gaattggtgg agaaagattt cctctatgga atgaaatggt
     1441 gactcaattt caagctatgg atttacctag gcaaatagaa gaaaagacac catatgaaat
     1501 aagagctttg ttttgtcatg ggataaatgc tagaatgttt actaattcag ggagactttt
     1561 taaagcgttg aatagtgtag atttttttgt gaatatagat ttatttatga cagatacatc
     1621 aaaatatgca gatatacttt tacctgcatg tacatcattt gagagagaag aatgtaagag
     1681 ttatccagga gggtatatta catataccaa aaaagttatt gataaattat acgattcaaa
     1741 atcagatgta gaaatacttt cagatttagc aaatgctatg aatatagatg atgatttatt
     1801 aaaagcaggt tatgaagcaa gtataagaca tatgtttaaa aatacatgtg tagatgtaga
     1861 taaacttaaa gaaagtgatt taccattaaa atgtaaagat tttaagccat atatagtagg
     1921 ttcatataca agagaagggt atgatactag cacttctagg tttgaattaa aatctacaat
     1981 tattgaaaaa tataaagatt ttgggctgga tgcactacca acatatagag gatttaatga
     2041 taatatagat gatgaatata ctcatttatt gacatcagga gcaagaattt caaatgcact
     2101 acattcaaga cttcatgaca tcaaactttc aagaatatta aaaaatgaag ctagttgtga
     2161 tatttctagc ttagatgcaa aagaattggg tgttatcagt ggagattata taaaaataac
     2221 tactaagata ggaagtttgg atgtaaaagt caatgttact gatagagtct tatataagaa
     2281 tatacatatg tatcatggat atagagaagc tgatataaat agtattattt gtgataattt
     2341 tgacccttat agtggattcc ctgcgtatag aagttgtaag tgtaagattg aaaaaatgaa
     2401 tagataaatg gagagatata atgaaaagtt tatttaaatt taataaagaa ttatgtacag
     2461 catgtggagc atgtttgctt gcatgtatag accaaaatga taataatata gacaatggtg
     2521 aactttttag acatgtattt acaatagagg aaggtgaaaa tatagaatat ttttcattag
     2581 catgtataca ttgtaatgag ccaaaatgtt taggtgtatg tcctaaaaat tgttttaaaa
     2641 aagaagaagg attcattgta ttagacaatc aaaactgtat agggtgtaaa ctttgtgaaa
     2701 aagcttgtga atatggagcc ttaatttatg gaaatgatgg taaggcaaat aagtgtaatg
     2761 gttgtattga gagatataag tgtggtatgg attcaccttg tgtaatggta tgtcctacag
     2821 gagcattaaa atttaataaa taaattaaat atagagaatg acttatactt aggtataaga
     2881 tatttttaca aaaagctgta ttgagtttgt tataatgaaa actcaatata gcttttttat
     2941 ggatatagtt ggatactata atgtgattct tagtaatagt tttttgctat tggacaaaga
     3001 ctgtaaaaca tattaaactt atgttgttca aagtcgaagt ataatataat aactagaaaa
     3061 ttggtttata gtgctataaa agcaaattta gtaaggtata aaatcagagg caatccttta
     3121 ctataatatc aatatttggg agttctgtta ttgcagagct attaaacaat tataatggta
     3181 caagtgattc aacctttgaa aatgtattat ataaaaaatt aaaataagat taaaaaggag
     3241 agaataaatt atgaaaaaac ttaaattaat tattatgttt gccttggtat cagcactgtt
     3301 agttacagga tgttcatcag gaaataataa aaataaagaa gaacaaaaag ttataagagt
     3361 tgcaacatca ggaacatatt acccttttgc ttttaaagat aaagataaac ttaaagggtt
     3421 tgaagtagat ttttgggatg aatttgctaa acgaactaat tgtaaagttg aatgggtatt
     3481 aactgatttt agtggattat ttggattatt agaagctgat aaagcagatg ttgtatctgc
     3541 tcaacttact gcaacacctg aaagagagaa aaaatatgct ttttcagatg cttataatta
     3601 tagtggaaca aacattattg ttagagaaga tgatactagt ataaaatcaa tagatgattt
     3661 agttggcaag aaagtaggtg tgggtactgg agcagtagca aatgaaatat taaaagaaaa
     3721 atacccaaat gatgaaataa aaatagtaaa ttattcaagt gcgactctta aagggaatta
     3781 tcaagaccta gaaatgggaa gattagatgc agtagttgcg caggatgttg aggctttaat
     3841 agctataaaa gaacaaaaat taaatcttaa aatgatagaa cctccaattc aatttggtgc
     3901 ctgttctttt gcgcttcaaa aaaatgaaaa aggtgaaaaa tggacaaaag aaattaacga
     3961 agtaattgaa gagatgtata aagatgggac tttgactaaa atatctgaaa actggctagg
     4021 aaaagacatt actaaggagc ctattaatga gtaattttaa ttttgaattt tgtctaagta
     4081 tatttccatt tttgattaaa gcattgggaa taacaatact aatatctatt gttgcatttt
     4141 ttttatcaat attattaggt gtaatacttg cctttttcac acagacaaaa tcaaaagttt
     4201 tacaatacat aacaagagtg tatgtttcat tctttagagg aacaccactt ttaattcaaa
     4261 tatttttctt ttattttgga tttccaatga tattttcttt tatgaaagat tgttcacctt
     4321 atttagcaat agtaatttgt ttaggtgtaa attcatctgc atatatggct gaaattatac
     4381 gagcatctat tgagagtgtt gataaaggac aacaggaagc atgttatgct tttgggttaa
     4441 gtaaatatca gtcaatggtt aaaataattc tacctcaagc agctacatct gcagtacctc
     4501 ctattgtaag ctgtacactg gacataatta aaggttcttc tttaggttta actataggaa
     4561 tacaagattt aatgggagta gctcaagtag aggctgcttc aagttttaaa tattttgaga
     4621 tatatatgat aacgatatta atttactggg cagtatctat aatattgagt aatatacaaa
     4681 gcaaagttga aaaaagaata agaagagcat attagatttc aatatttaaa aatgggatga
     4741 tatagatata ttattacttg taaataaatt tttaatttta gaaaaataaa cttagtaatc
     4801 taaattattg agaaaatata gtatcaaaat ggactgtata atcaagttaa aaaatagttt
     4861 ttctattgaa aaaataaata aaattctatt gtaatttaat atgcgataaa gttttataaa
     4921 aaacaacatt aagctagggg gacttatttt atggctttta atatgaaata ttttttagaa
     4981 ttatttccaa caataatgaa atcgttcaat gtaactgtga ttttggcaat aagttcatta
     5041 gtattttcat taattatagg tacaatagta gcgttatcat cttattacaa agtgaaagta
     5101 ttgaatcaaa tatgtaaagt atatgtatct atttttagag gtacacctct tatgccacag
     5161 ttattctttt tatattttgg actagcatat atgagtgatt ttgttaaaaa tatggaccca
     5221 gtatttgcaa catctatagt acttagttta aatatgggag cttatatgtc tgaaacgata
     5281 agggcatcta tactttcaat tgatacaggt cagatagaag cagcatatgc aatgggatta
     5341 acaaatttac aaacaatgaa aaggatagtg ataccacagg ccgtaagagt tgcactgcca
     5401 tcattattca ataattttat agatttaatt aaagggtctt ctgtagcttt tgttgttgga
     5461 gtaaatgata taatgggtgc tgccaaatca caaggagcgt tgagttttaa gttttttgaa
     5521 gtgtatggag cagttatggt aatttactgg tgtataataa ctcctttagg ttgggggcaa
     5581 aaaaaattag aaaagaaaat aagcaattat taagttatta agaaaataca taaaagtatt
     5641 tttatataaa aaatggtgtg aggagaagag atatgataaa aagaaatatt ataaaatgtt
     5701 tagtggcagt tttatcgtta acgtttgtaa ttactggttg ttcaaaaaaa gaggaagtaa
     5761 agaatgaaaa agttataact gtagcttcat ctaattcagc tgacccatat tcttatgttg
     5821 ataatggtga gcataagggt tttgaagttg acatgtggaa agagattggt aaaagaagtg
     5881 gatataagat aaaaatggaa ccaacaggtt ttagttctat atttggtatg ttagattctg
     5941 gtaaagttga tgtggcagcc aacttctttg gaatgtctaa agaaagatta gaaaaatacg
     6001 atgcttctat accttatggt tctgattctg tgtgtatagc tgtaaaagat ggaaataacg
     6061 aaattaataa gcttgaagat ttaaagggaa aagttgtagc agtttcagaa ggttctcaag
     6121 gtcaacaggt tgcaactagt gttaataaag ataatttgtt tcaagaaaag gtttacggtg
     6181 atggaactag tggtcttcaa gatttagatt tgggaagagt tagtgcatgg attgatgcag
     6241 agcttactat tgtgggagac gctaaaaaag ctaatatgaa agtaagagta ttagataaaa
     6301 aattatcagc tactaatata gcatacttct ttaagaaaaa cgatgataaa tctaaaacga
     6361 taaaagaaga tgtaaacaaa gcaatagaag aaatgcttgc agatggaaca gtgaaaaaaa
     6421 tatctgaaaa atggtttgga atggatgtaa gtgctgatat acaaaaataa atactttaaa
     6481 attaaaatgt aaaactgtga taactataga taagtatact aaatttatct atagttatct
     6541 gtgtattaca atgtatttag attagtcttt ataaatcaaa atgagttatg gaggtattgc
     6601 aaatgattga aactaaaata aaatccattg cactaaaaaa tagagaaaaa attataaaaa
     6661 taagacgaca gatacacagt aatccagagt tagcatttaa agaatataaa acatctaaac
     6721 ttataaaaga agagctaaat aaacttaata tagaatacat aaatattgct ggtacaggag
     6781 tattagctac tataaaagga aaaaataatg gtggaaaaac tatattactc agagcggata
     6841 tggatgctct tccaatcaaa gaggaaaatg atttagaatt taaatcaata aatgataata
     6901 tgcatgcttg tgggcatgat gcacatgttt catggttgtt aggaacagca atgattttaa
     6961 atgacataaa agaagaattg aatggaaatg taaaattact atttcaacct ggcgaagaaa
     7021 aaggtggttc tgatattgta attaaagaaa atgtacttga aggtgtggat gcattagcga
     7081 ctggtcattg ttggccaact attgagtcag gaaaaattgg aatcgctaga aattgtgcta
     7141 tggcagcgac taatactttt gaaataacaa taattggcaa gggtgggcat ggagcagagc
     7201 ctcataattg tatagaccca attgcagttg gaaatgcagt atattcatcc attcaacaga
     7261 tagtttcaag aaaaattgac ccagttgtac ctgtagtagt gtctgtttgt tcttttaatt
     7321 ctggtgtttc taaaaatata ataccagatg tatgtacact tcaagggact attagagcga
     7381 taagcaagga acaagtaatt gaagtaagta aaattctaga atatatagta aaaaatgtat
     7441 gtaaagcaca tggagcagat tgcaaatttg aaaagagtac aggaggagat gctgttataa
     7501 atgacaaaaa catgatagag ataggaagag aaagtgcttg caaaatactt ggagctgaaa
     7561 atgttgaaat aattgatttt cctgcaatga caggtgagga ttttgctatt tatatgaaag
     7621 aaaaacctgg tttatttatg tatataggtg taggagataa agaaaaaaat ataaattaca
     7681 gattgcatag taacaagttt aatatagatg aaaaatgtct tagtatagca ccttcattat
     7741 tttcacaact tacagtagat tatttatgtc aactatagag tacattcata aaaatatatt
     7801 tagcctattt ttaaatttaa tgctagtata ttaattttga tgtagaacat attatttatt
     7861 atatttattt aaaaaggtgg ataacaatga taaaagtaga aaatttacat aaaagtttta
     7921 aagggacaga agttcttaaa ggtataaatt tagagataag tcaaagtgaa gtagtagcta
     7981 tacttggatc atctggaaca ggtaaatcta cgttacttag atgtttaaat tatttagttg
     8041 agcctacaaa aggaaagata atgattggtg atgtaaaagt agatgtagaa aaagcaacaa
     8101 aaaaagatat aaccaattta agaaaacata catctatggt gtttcaaaat tataacttat
     8161 ttaaaaataa aacagcatta gaaaatgtaa tggaggcatt gatagtagtt cataaaaaga
     8221 gtaagaaaga agctagagaa atatctttaa aattaataga taaagtaggt atgttggata
     8281 ggaaagattt ttatccatca aagttgtctg ggggacagca acaaaggata ggtatagcga
     8341 gagcgatggc agtaaatcca aatgtaatat tatttgatga gcctacatct gcacttgacc
     8401 cagaacttgt tggagaagtt ttaagcgtta taaaatctct tgcaaaagaa ggtataacga
     8461 tgcttattgt aactcacgaa ataaattttg caaaagaagt tgctgataaa attatattta
     8521 tggataatgg tgtaatagct gaagaaggaa ctcctgatga aatatttaat aatcctaaaa
     8581 atgaaagaac agcaaaattt ttaaatatca ttaggaaaga agaagtattt agttaaaagt
     8641 gtataaatta agcattagaa gatatgtaag atacccccat taaatttata agttagataa
     8701 taaatttaat gggagtatat ttttattatt agatttatta tttaatttaa aattagaaat
     8761 gtaactattt gaaaattctt tttaaaagta tatcaaaata tcaaatacat aaaaaaataa
     8821 tatttgacaa agtatcatat gtgtttacaa aat
//
LOCUS       DAEPIN010000108         8827 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.110, whole genome shotgun sequence.
ACCESSION   DAEPIN010000108 DAEPIN010000000
VERSION     DAEPIN010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8827
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.110"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            <1..702
                     /locus_tag="KR250_003407"
     CDS             <1..702
                     /locus_tag="KR250_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HBH0069293.1"
                     /translation="MYIVSIIIAFLLKYILKCNDKKDLGIYQYIVVFSNVAFMGYPVI
                     EAVLGHEAIFYTAIFNLPFNLFSFTLGIYLLSKGSTSRGFSIKSLISPATIAIVIGLF
                     LFVTGLRLPQFINEPLEMLGSITTPISMIIIGSLLANSSALDCFVNKKLYIVTFIRLL
                     VLPVIVYFILKGWINDKMILAIPVVISSMPAAANTAIMANQYDSNITLASQCVFFTTL
                     FSVISIPFISILLLS"
     gene            841..987
                     /locus_tag="KR250_003408"
     CDS             841..987
                     /locus_tag="KR250_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069294.1"
                     /translation="MDMDLLFKIVFAIVAIGVSVKIIKTVASLAFKIALILLIILFVY
                     RMFL"
     gene            complement(1092..1616)
                     /locus_tag="KR250_003409"
     CDS             complement(1092..1616)
                     /locus_tag="KR250_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBH0069295.1"
                     /translation="MSKPIDITIRPIRREDAQAFNEIRRTYSVMRNTLALMSDRVDKT
                     EAMLASLGENDYMFVAETNNNGEKDVIGFAGLHVNSSPRLRHSAEIGISVAENYQGRG
                     VGKKLMEQLLDIADNWIMLTRVGLEVIVDNEKGLNLYKKLGFEIEGTKKYAVIRDGKF
                     EDVYIMGRYNKNLI"
     gene            complement(1669..1893)
                     /locus_tag="KR250_003410"
     CDS             complement(1669..1893)
                     /locus_tag="KR250_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069296.1"
                     /translation="MIIKLTKDNFNFRFKQYEGMFFHAEVVEGMESIRYKITKDKEGT
                     ELKEFGSTHIKDNMIYVPQVEAYIDLNSIK"
     gene            2090..3214
                     /locus_tag="KR250_003411"
     CDS             2090..3214
                     /locus_tag="KR250_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3810 domain-containing protein"
                     /protein_id="HBH0069297.1"
                     /translation="MSKKTKYFSLILFPIALLLNFIASKVPNIVEKYYSQFIDKIIVQ
                     ILSKVSGIFPFSLYEITMYIIVISIFLFLCCTLSTIFNKKKKLNIYLKNSILNILSIV
                     SIVYFLFVVLWGINYNRIPLETTLINNYNLKNNTSIQSKQYSVKELTKLYKFLVIKAN
                     ETRKLTLQDKNGIVKSNTDYNGVINRAQLGYDNILDILPGVSGSYSKPKYIISSNLMC
                     YTGITGIYFPFTGEANVNIAIPDLYIPCTVEHEMAHQRGFASEDEANFIAYLTSIKHP
                     NIDFNYSGYILALNYTASALSKVDYNAYVDISAGISDSVRRDLKNESEFWQKYEGKIN
                     EISNEFNNSYLKANSVTEGTQSYGKMVDLLLTYYELYPYN"
     gene            complement(3688..4512)
                     /locus_tag="KR250_003412"
     CDS             complement(3688..4512)
                     /locus_tag="KR250_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069298.1"
                     /translation="MRYKKISSLPILVVALLITGTTTTFSLSTTNSEKTNVYLANTVS
                     TVTKKVSDIQSSKGLVYNENIPLFINNSKIQYDDTPYHWPSNVISLTNSSEKAIMDYE
                     ITCLAYDKNGKPLELYWDAQNVAADGEVGSVGFSPAGVDYGIVTGISPVSPKSYSHTY
                     RKMQQSPPQDIISMFEKQQGKAWVENWLKEWKQMEKEYAKQNAIAPGKNQNDAFLLFD
                     KWKQSTGEHGVKYIISCVKQVTFNDGSVWKNSAYENWLKSFQGKEVSNSVLENYYK"
     gene            complement(4860..5915)
                     /gene="ispG"
                     /locus_tag="KR250_003413"
                     /gene_synonym="gcpE"
     CDS             complement(4860..5915)
                     /gene="ispG"
                     /locus_tag="KR250_003413"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HBH0069299.1"
                     /translation="MSYERRKTREVSVGTVKIGGENPISIQSMTNTDTRDADATIAQI
                     KRLEEAGCDIVRVAVPDIKAAKNIAKIKSSVNIPIIADIHFDYKLALEAIEQGVDGIR
                     INPGNIGSIERVKMVVEKCKERNLKIRIGVNGGSLEKELLKKYGSATAEALVESAMGH
                     IKILEDLDFHNIVISLKSSDIYKTVDAYELISKKVDYPLHIGITESGSVHKGTIKSSI
                     GVGALLLKGIGDTVRISLTGDPVEEVIVGKQILRSLGLLNDKIKVISCPTCGRCNIDL
                     ISVVNEVEEKIGSMEKNITVAIMGCAVNGPGEAREADIGIAGGKGEGLLFKKGEIVRM
                     IDGNKLVDELLEEIEKL"
     gene            complement(6097..7101)
                     /gene="rseP"
                     /locus_tag="KR250_003414"
     CDS             complement(6097..7101)
                     /gene="rseP"
                     /locus_tag="KR250_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HBH0069300.1"
                     /translation="MTIIAALILFSIIVLIHELGHFIFAKRSGIKVNEFSIGMGPKIY
                     SVKKDTEYSIRALPIGGYVSMEGEDEEQISPNSFGNKSILQRFSTIVAGPIFNIILAA
                     ILLVPVFLYIGSPTTKLGKIMPDTPAQAVGLQVGDKINKINGNSVKTWDEVANIINTS
                     SGGELKLSITRDGSDKVVNVTPKNNNGKYEIGIQPQREKDFLGSIVNACKTTVDMTKQ
                     MLTFLGQMITGRVPGGIGNAVAGPVGVIGMVSDAARTGIINVVYLAAVISLNLGIVNL
                     LPIPALDGWRILMLLLEAVRGGKKLDPNKEGMINVVGFGALMLFMLFITYKDILRLFQ
                     "
     gene            complement(7114..8268)
                     /locus_tag="KR250_003415"
     CDS             complement(7114..8268)
                     /locus_tag="KR250_003415"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HBH0069301.1"
                     /translation="MKKISILGSTGSIGKQTLDVVRDNRDKFEIVAISANSNIELLLE
                     QIVEFKPKYVTVFEENKALKLKEMLPKNIEIEVLAGMEGLKIISSLDEVDVLLTAVVG
                     MIGLVPTLCAIKKGIDIALANKETLVTAGELVMKEAEKYNVNILPVDSEHSAIFQCLN
                     GENKKNIEKIILTASGGPFRGKKKGELANITKNEALKHPNWSMGRKISIDSSTLMNKG
                     LEVIEARWLFGVEQENIDVVVHPQSIIHSMVQYTDSSIIAQLGCPDMRLPIQYALTYP
                     DRMESSFERMNFSKFSTLTFEEPDLETFPCLKLAYECLKMGGTYSSVLNSANEVLVSE
                     FLEDKIGFYDIPYYIEKTLEVHSSISEPTLEEILETDRWSRAYVANLIKK"
     gene            complement(8432..8635)
                     /locus_tag="KR250_003416"
     CDS             complement(8432..8635)
                     /locus_tag="KR250_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH0069302.1"
                     /translation="MISYAPLWKLLIDRKIKKMEFVNISGISISVLGRLGNDKSVSMD
                     TMEKICLALDCKIEDVVEIKKSI"
BASE COUNT         2913 a         1505 c          910 g         3499 t
ORIGIN      
        1 atgtatatag tttcaattat aattgcattt ttattgaaat acattttaaa atgtaatgat
       61 aaaaaagatt tgggaatata ccagtatata gttgtttttt caaatgttgc ttttatgggt
      121 tatcctgtaa ttgaagcagt tttaggtcat gaagcaatat tctatactgc aatattcaat
      181 ttgcctttta acttattttc tttcacttta ggaatttatc ttttatctaa aggcagtaca
      241 agtagaggtt tttcaataaa atcacttata agccctgcta caatagctat tgttattgga
      301 ctttttttat ttgtaacagg acttaggtta ccccaattta taaacgagcc acttgagatg
      361 ttgggaagta taactacacc aatatccatg attatcattg gaagcttact tgctaattct
      421 tcagctctcg attgttttgt caacaaaaaa ttatatatag tcacttttat aagattattg
      481 gtacttcctg tgattgtgta ttttattcta aaaggatgga taaatgataa gatgatttta
      541 gctataccag tagtaatttc ttcaatgcct gctgctgcaa atactgctat aatggctaat
      601 caatatgact ccaatataac actagcttca caatgtgtgt tttttacaac actattttca
      661 gttatatcta taccttttat aagtatttta ctcttatctt gatttatttt tttatgtatt
      721 atataataac atattcaata aattacaaat ataaacattt tattgtagta tattgatgga
      781 attttttcct agatatttgt caataatatc tatatgtatt atagaatagg aggcttaaat
      841 atggatatgg atttactctt taaaatagta tttgctatag ttgctatagg tgtttctgta
      901 aaaatcatta aaactgttgc tagtttagct tttaaaatag cgttgatatt gctaataatt
      961 ttatttgtat atagaatgtt tttgtaaaca tttaaaaata agttttttaa cacaatagag
     1021 aagtaaaata gattatcttt aatgtaaatt ttttattaaa gacactctat tttacttctc
     1081 tattagctta tttaaattaa atttttatta tatcttccca taatataaac atcttcaaat
     1141 tttccatctc tgattacagc atatttttta gttccctcta tttcaaaacc tagcttttta
     1201 tataaattta atcccttttc attatccaca attacttcta atcctactct agttaacata
     1261 atccaattat ctgctatatc taaaagttgt tccataagct tcttacctac tcctcttccc
     1321 tgataattct ctgcaacact tattcctatt tctgcgctat gtctcaatcg tggagaagaa
     1381 tttacatgta acccagcaaa acctattaca tctttttctc cattattatt agtttcagct
     1441 acaaacatat aatcattttc tcccaaagaa gctaacatag cttcagtttt atcgactctg
     1501 tcactcatta atgccaaagt atttctcata acactgtatg ttcttctaat ttcattaaaa
     1561 gcttgtgcat cttctcttct tataggtctt attgttatat ctataggctt actcatattg
     1621 gattccccct caaacataat ctttattttt aattaaaatt ttacatcttt attttatact
     1681 gttcaaatct atgtatgctt ctacctgagg tacatatatc atattatctt ttatatgtgt
     1741 acttccaaat tcttttaatt ctgtaccttc tttatcctta gttattttat atctaatgga
     1801 ttccatacct tcaactactt cagcatgaaa aaacatacct tcatattgtt taaatctaaa
     1861 attaaaattg tctttagtaa gttttattat catattttta tctactcctt atataattga
     1921 tatttttctt attattagtt gtatatatac tgcttattca tattaatata ttataactta
     1981 tcatttattt agtataaagt tataaataga tatatttata cttattttac aatctttctt
     2041 caattgatat ataatatgtt tatataaact aatgtatgtg aggtgaaata tgagcaaaaa
     2101 aacaaaatat ttttctttaa ttctctttcc tattgctttg cttttaaatt ttatagccag
     2161 caaagttcca aatattgttg aaaaatatta ctcccaattc atagataaaa taattgtaca
     2221 aatattgagt aaagtttctg gaattttccc attttctctc tatgagatta caatgtacat
     2281 aatagttata tctatcttct tatttttatg ttgtacttta tctacaattt ttaataagaa
     2341 aaaaaaatta aacatatatt taaaaaattc cattttaaat atattatcca ttgtatcaat
     2401 agtctacttt ctttttgtag tgctttgggg aataaactat aataggattc cactagaaac
     2461 cacacttata aataactata atttaaaaaa caatacctct atccaatcaa aacaatatag
     2521 tgttaaagag cttactaaat tatataaatt ccttgtaatt aaagcaaatg aaactagaaa
     2581 actcacatta caagataaaa atggtattgt aaaatctaat acagattata atggtgtcat
     2641 aaatagagct caactaggat acgataatat cttggatata ttacctggtg ttagtggtag
     2701 ttattctaag cctaagtata ttatttcatc aaatctaatg tgctatacag gaataactgg
     2761 tatatacttt ccttttacag gtgaggcaaa tgtaaatata gcaatacctg acttatatat
     2821 tccttgtaca gttgaacatg aaatggcaca tcaaagggga tttgctagtg aagatgaggc
     2881 taattttata gcgtatctta cttctataaa acaccccaat attgatttta actattctgg
     2941 atatatttta gctttaaatt atactgcttc tgctttaagt aaggttgatt ataatgcata
     3001 tgttgatatt tctgctggca tttcagattc tgtccgtaga gatttaaaaa atgaaagtga
     3061 attttggcaa aaatatgaag gtaaaattaa tgaaatttct aatgagttta acaattctta
     3121 cttaaaagct aatagcgtta ctgaaggtac tcagagttat ggtaaaatgg ttgatttatt
     3181 gctaacttat tatgaattat atccatataa ctaaacacta caattcaaaa ttttattcat
     3241 atacagctga tattaaatca gctgttttct atttaaataa attatttaac ttcaagcaat
     3301 cttatataat ttgatagtac ttatactatc tgtataacat ttcaattcta actattttag
     3361 ttaaaacatt tctattatct tttttaataa atgttttaac taaaattttt aaaacataaa
     3421 acttccacat tatttcacta tctatataaa aatctaaaaa ttcacatata aaattacata
     3481 actaaaaaat cataaattta tagaaaacat atattcaaaa taaaaaagca tgtatcaaaa
     3541 cttgacacat gcttataatc ttatgtatat atatcatttt gtcacaataa aaatttactt
     3601 gtaacactga tatttttaaa caaaatagtc cttatttaat taaattaaca ttaattaaat
     3661 tcgtactcat tttgctatca ttaaacatta cttatagtaa ttttctaata cactatttga
     3721 aacttccttc ccttgaaaac ttttcaacca attctcatat gctgagtttt tccatacact
     3781 accatcatta aatgttactt gttttacaca agatataatg tactttacac catgctctcc
     3841 agttgattgt ttccatttat caaacaatag aaaggcatca ttttgattct tacctggtgc
     3901 tattgcattt tgctttgcat attctttctc catttgtttc cattctttca accaattttc
     3961 tacccatgcc ttgccttgtt gtttttcaaa catacttatt atatcctgtg gtggtgattg
     4021 ttgcattttt ctataagtat gtgaatatga ttttggagac acaggagata tgcctgtaac
     4081 aataccatag tctacaccag ctgggctaaa tccaacactt cctacttcgc catctgctgc
     4141 cacattttga gcatcccaat atagctcaag tggttttcca tttttatcat atgctaagca
     4201 tgtaatttca taatccataa ttgccttttc actgctattt gttaagctta taacattgct
     4261 aggccaatga tatggagtat catcatactg tattttgcta ttatttataa ataatggaat
     4321 attctcatta taaacaagac ctttactact ttgaatatct gataccttct ttgtgacagt
     4381 acttaccgta tttgctaagt atacatttgt tttttctgaa ttggtcgtac taagagaaaa
     4441 tgttgttgtc gttccagtaa tcagcaaagc aacaactaaa ataggtaagc tagatatttt
     4501 tttatatctc acaattcttc acctcttttt ctattaactt ataataaaac aatttaccaa
     4561 aacattaaaa tctatgttaa cataatttac caactgttct agtaactata tcctatcata
     4621 gttactatca agtttcaata ctattgatgg aaattttcac tatattaaat ctttttttaa
     4681 ttttataaat ttacaatatg atttaactca tacatatata gtcaaattag aatataagtt
     4741 tcttacattt aatgaagttt ataaaataaa aaatgactag ataattaaat actgtaaata
     4801 aaaacttatt acaaaagtat ttaaaaatct agtcaattaa tatatatatg aattgtatac
     4861 tataattttt caatctcttc tagcaactca tcaactaatt tgttaccatc aatcattcta
     4921 actatttcac cctttttaaa taaaagacct tcacctttac ccccagcaat acctatatct
     4981 gcctctctgg cttcaccagg tccatttaca gcacagccca taattgcaac agtgatattt
     5041 ttttccatac taccaatctt ttcttcaacc tcatttacaa cactaattaa gtctatatta
     5101 catctaccac atgtagggca tgatataact ttaatcttat catttaaaag tcccaaactt
     5161 cttaaaatct gtttaccaac aataacctct tcaacagggt cgcctgttaa agatattcta
     5221 acagtatctc caattccttt aagaagaagt gctcctaccc ctatagaaga ctttattgtt
     5281 cctttgtgaa cacttccaga ttctgtaatt ccaatatgaa gaggataatc aacttttttt
     5341 gatattaatt catatgcatc aacagtttta taaatatcgg atgattttag agatataact
     5401 atattatgaa aatctaagtc ttcaagaatc ttaatatgtc ccattgcact ttcaactaat
     5461 gcttcagcag ttgcagagcc atactttttt aaaagttctt tctctaaaga acctccatta
     5521 actccaattc taatttttaa atttctttcc ttgcatttct caactaccat cttaactctc
     5581 tctatacttc caatattacc aggatttatt cttattccat ccacaccttg ttctatagct
     5641 tcaagggcta gcttataatc gaaatgtata tctgcaataa ttggtatatt tacactagac
     5701 tttatctttg ctatattttt agcagcttta atatcaggaa cagcaactct aactatatca
     5761 caaccagctt cctctaatct ctttatttga gctattgtag catctgcatc tcttgtatca
     5821 gtatttgtca tagattgtat acttatagga ttttctcctc ctatttttac agttcctaca
     5881 cttacttctc tcgtctttct tctctcgtaa ctcatattcc acctctatat attattttat
     5941 attgaatctc tttaaatatt aaaatattat aacctacatg tatattataa attaaaaagc
     6001 tatctagtta aataagaata aatttcatat ataaaaatta atttattctt atttaaaact
     6061 acatagccta ttttcttaaa atctctaaaa actaaatcat tgaaataatc ttagtatatc
     6121 tttatatgtg ataaaaagca taaataacat taaagctcca aaaccaacaa catttatcat
     6181 gccttcttta tttgggtcga gttttttacc acctctaaca gcttctagta acagcataag
     6241 tattctccat ccatcaagtg ctggtattgg taatagattt actataccta aatttaagct
     6301 tatgacagct gctaaataca ctacatttat gatgcctgta cgagcagcat ctgataccat
     6361 accaataaca cctactggtc ctgctacagc attgccaatc cctcctggta cacgtcctgt
     6421 aatcatttga cctagaaatg ttagcatctg ttttgtcata tctacagtag ttttacatgc
     6481 atttactatt gaccccaaaa agtctttttc tctttgtggt tgaatcccta tttcatactt
     6541 tccattgtta tttttaggtg ttacatttac aactttatca cttccatctc tagttatact
     6601 aagttttaat tctccaccag aagaagtatt tattatattt gcaacttcat cccaagtctt
     6661 aacagaatta ccattaattt tatttatctt atctcctact tgaagcccta cagcctgtgc
     6721 aggagtatct ggcattatct ttcctaattt agtcgttgga gaacctatat acaaaaatac
     6781 tggaactaaa agtattgctg ctaatattat attgaatatt ggtcctgcta caatagtact
     6841 aaatctttgt aatatagact tgtttccaaa agaatttgga cttatttgtt cttcatcttc
     6901 accttccatg ctgacatatc caccaattgg aagtgctctt atagaatact ctgtatcttt
     6961 ttttacacta tatatcttag gccccatacc tatagaaaac tcatttactt tgataccact
     7021 tctttttgca aatataaaat gtcctaactc atgtattaat acaattatac taaatagtat
     7081 taatgcggct attatagtca taaattcacc ttcctatttt tttatcagat ttgcaacata
     7141 agctctactc catctatctg tttctaagat ttcttctaat gttggttcac ttatgctact
     7201 atgaacttct aaagtcttct ctatgtaata tggtatgtca tagaaaccaa ttttatcctc
     7261 taaaaattca cttactaaaa cttcatttgc tgaatttaaa acagaggaat aagttcctcc
     7321 catttttaaa cactcatatg ccaattttaa acatggaaaa gtttctaaat ctggttcttc
     7381 aaaagttaaa gtactaaatt ttgaaaaatt cattctttca aagctacttt ccattctatc
     7441 tggatatgta agagcatact gtattggtag tctcatatct ggacatccta attgagcaat
     7501 tattgagcta tctgtatact gcaccattga atgaattatg ctttgtgggt gaactactac
     7561 atctatattt tcttgttcta ctccaaataa ccatctagct tctatcactt caagtccttt
     7621 attcataagc gttgaagaat caatacttat ttttcttccc atactccaat ttggatgctt
     7681 caaagcttca tttttagtta tatttgcaag ttctcccttt ttctttcctc taaatggacc
     7741 accagatgct gtaagtatta tcttttctat atttttttta ttttctccat ttagacattg
     7801 aaatatagca ctatgttcac tatcaacagg aagaatattt acattgtatt tttctgcttc
     7861 tttcattaca agttcaccag cagttaccaa cgtttcttta ttcgctaatg ctatgtctat
     7921 tcccttcttt atagcacaaa gagttgggac taatcctatc atacctacta cagcagttaa
     7981 aaggacatcc acttcatcaa gtgatgatat tatttttaat ccttccattc ctgctaaaac
     8041 ttctatttct atatttttag gcaacatttc ttttaatttc aatgctttat tttcctcaaa
     8101 aactgttaca tatttaggtt taaactctac tatctgttct agcaataatt caatattact
     8161 gttagctgat attgcaacta tctcaaactt atctctatta tctctaacta catccaaagt
     8221 ttgtttacct atagagcctg tagaacctaa aattgatatc tttttcatat aataatcctt
     8281 tctaataata aatataccct gtcaaaacat tataacacta ctaaaacaat tatttttact
     8341 attcatagaa atttcatata ttattccaca gattaaaatt aatctattat tttttgtatt
     8401 ttgtggaata ttataaaata ttctagtatt attaaatgct cttttttatt tccacaacat
     8461 cttctatttt acagtctagt gctaaacata tcttctccat tgtatccata gaaacagatt
     8521 tatcatttcc tagtctgcct aaaacagaaa tacttatacc tgatatattt acaaattcca
     8581 ttttctttat ttttctgtca attaacaatt tccataatgg agcgtatgaa atcatgttat
     8641 cacctcgcaa aatatattct atcacatttt atctattttg gaaaattttc atctatttag
     8701 ataaataatt atttatctaa atagatttca attatagttt attaaatata aatatgttaa
     8761 aataaaacaa aagcataagt tcaaaaatta aactttatat atatttatct cattatttta
     8821 ctaaaac
//
LOCUS       DAEPIN010000109         8466 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.111, whole genome shotgun sequence.
ACCESSION   DAEPIN010000109 DAEPIN010000000
VERSION     DAEPIN010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8466
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.111"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            complement(332..1228)
                     /locus_tag="KR250_003417"
     CDS             complement(332..1228)
                     /locus_tag="KR250_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBH0069303.1"
                     /translation="MKDKNTYFNRKGQENFIDIFLKDDIKKGANFKRHWHEHLQIYYF
                     IDGIAYLECGLDNFKVVPGNIVIANSNELHYLESLSDNLKFYSIRIEPTFLFSNQVDL
                     LQTKYLAPLALNHIVFNNLIQNDSLILKCITTILDEYHNKEVGYELSIKANIYQLIVL
                     LLRSHIDKIYSEDEFIKRKYSLEKFECIFDLIEEKYMDKITLKELASRVNLSAHHFCR
                     TFKQITGKTTIDFINGIRLDKAIYYLKETDLNMTEIAMRCGFDSINYFSRLFKKNYNI
                     SPTKYRNEHREVSNVILKEDMK"
     gene            1369..2796
                     /locus_tag="KR250_003418"
     CDS             1369..2796
                     /locus_tag="KR250_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBH0069304.1"
                     /translation="MTNSFKKDDNRKKITVLFVVIAMTFMATLDSSIINVALPVLASK
                     LNVSLASIEWVIASYSIIICSTLLFFGRLGDIIAKSRVFQVGTILFTSASLLCGLSNS
                     LTLLIVCRFIQGIGASAYMANNHGIITELFPKESRGKALGILVTAVAIGNMVGPSVGG
                     FILSIFNWNVIFFINIPIGLIVIFLNTKFLPNSKKSSENMDKTGAILQFLGTTLFFSA
                     LISAQQTGLLNPYILIALLLSIIFIILFLILEKKHPQPLLDLEIFRNFKFSLNLICAL
                     TSFICIASSSILIPFYLQSTMKLPPIQAGLFMILSPLILAIFSPIFGNISDKIKSEKI
                     ILIGLLVMSFGFFLMSRLKESSALILFVIYISIISIGQAIFQPANNALIMSSCSRSKL
                     GVVGSINSLVRNLGQVIGITISTTLLYNFMSIKAGYRVNDYVINNDKIFVFGMRNVYI
                     IVTLVCLIGAILIGFYLFKYNKNEQ"
     gene            complement(2992..5586)
                     /gene="clpB"
                     /locus_tag="KR250_003419"
     CDS             complement(2992..5586)
                     /gene="clpB"
                     /locus_tag="KR250_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HBH0069305.1"
                     /translation="MDVEKMTLRVQKSLNEAYNEAVKNHNQQVDVIHLFSALINQEDG
                     LIPNIIEKMNISIDSLRNTVNFEIDKLPKVYGEGADSQGVSATRKINEVLIKAESISK
                     EFKDSYISVEHVMLAMMETESKSAVGKILKQYNINKNDFLNILSQVRGSQRVETQDPE
                     GTYDALARYGTNLVDLAKKNKLDPVIGRDEEIRRIIRILSRRTKNNPVLIGEPGVGKT
                     AIVEGLAERIVRGDVPEGLKDKIVFSLDMGALIAGAKYRGEFEERLKAVLKEVQSSDG
                     KIILFIDEIHTIVGAGKTEGSMDAGNLIKPMLARGELNCIGATTFDEYRKYIEKDKAL
                     ERRFQPVIAEEPTVEDTISILRGLKERFEIHHGVRIHDNAIVAAAKLSHRYIQDRFLP
                     DKAIDLIDEAGAMIRSEIDSLPTELDVVRRKLFTLETEREALLKENDDKSKSRLDDIQ
                     KEIAELKSKNDEMTAKYEKEKSQILDIKNLKAQLDEAKGKAEKYEREYDFNKAAEVKY
                     GEIPKLEEQIKQYEENMNDGSENSLLKEEVTEEEISSIVSKWTGIPVTKLVEGEREKL
                     LKLEDELHKRVIGQDEAVTAVSNAVIRARAGLKDERKPIGSFIFLGPTGVGKTELAKT
                     LARNLFDSEDNIIRIDMSEYMEKHAVSRLVGPPPGYVGYEEGGQLTEAVRRAPYSVIL
                     FDEIEKAHEDVFNMFLQILDDGRLTDNKGKTVDFKNTLIIMTSNIGSNYLLEAGGNIT
                     ETTNNLVMNEMKHRFKPEFLNRVDDIIMFKPLDQENIKKIIDIFMKDLKNRLKEKDIT
                     IEVTNSAKDVMVREGYDPVYGARPLKRYIGNTLETIIAKKLIAGDVYNGCTIVIDGKD
                     ENIEVLVK"
     gene            complement(5623..6219)
                     /locus_tag="KR250_003420"
     CDS             complement(5623..6219)
                     /locus_tag="KR250_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069306.1"
                     /translation="MSLKLDRNVLQWFDYVFENEKTSLRHYNFNCTLKEISSTSLNKV
                     AFILEKNNSKYWKLYFEIPAEVTLKLKQNIHPLFREYIYEQISLYNNNQIYNFVNSNI
                     LKVFNNIAIYQYNILENLYTIDFKKSFIDKCQYLLIGEKRLIDEDLYLIAKSKEVFDF
                     FNSDGTFNLTLSFDIQKNENLLDSLLELRKSIIINERI"
     gene            complement(6236..6640)
                     /locus_tag="KR250_003421"
     CDS             complement(6236..6640)
                     /locus_tag="KR250_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="HBH0069307.1"
                     /translation="MDKTLKKDLNQEYTESKSLVMNKSKYMNVCFKNYIKVVEGMYTK
                     RDLIPLKLINSMDKNNKIRKTLYELSCEFEYPKTSLSSLFTELKKMDFLQRVKNGEYM
                     INPHIAYKGSRADKESLLAEYNQIKRPNKVSK"
     gene            7007..7381
                     /locus_tag="KR250_003422"
     CDS             7007..7381
                     /locus_tag="KR250_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBH0069308.1"
                     /translation="MNIVISNSSGKPIYEQITTQIKSMIISGELPEGSALPSMRLLAK
                     ELRISVITTKRAYSDLERDGFIETVTGKGSFVSSKNMDFVKEEQFRLIEEYLQKAVDT
                     AKSSDITYDELKDILFLLYKGE"
     gene            7378..8241
                     /locus_tag="KR250_003423"
     CDS             7378..8241
                     /locus_tag="KR250_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0069309.1"
                     /translation="MISMDAITIKNLNKTYKDFSLQDISFSVPKGSVMGFVGENGAGK
                     TTTLKAILNLISYDSGNIEIFGLDSKKNEKEIKEQIGVVFEGSNFHENLNTDHVSKIM
                     SKIYKNWNDTLFKDYLKKLRVPDNKLIKEFSKGNKMKLSIAVALSHKPKLLILDEATS
                     SLDPIVREEILDIFLDFIQDEEHSIILSSHITSDLDKIADYITFIHKGKIVFSENKDE
                     LIDTMGVLKCKPSDFDNLSREDYSYYRKSQFGYEVLLKDKHRFISRHPNCIVDNTSIE
                     EIMLFYVRGDK"
     gene            8241..>8466
                     /locus_tag="KR250_003424"
     CDS             8241..>8466
                     /locus_tag="KR250_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HBH0069310.1"
                     /translation="MKGLILKDLLNLKGNIKFILLFIIMFGFMSSLGDGNVNNFIGVI
                     IVLCTTMIVSTFSYDDLNKWDSYVLTMPINR"
BASE COUNT         2839 a         1263 c          975 g         3389 t
ORIGIN      
        1 atttaaattt tctataaaaa tcatactatt ttagctgttt acttgcttat ttaatagttt
       61 tatttctttg attaaatatc tttcagaatc taagccagat gtctcaaaaa aactgattta
      121 taatataatc attttttctg ctattcttat ttttccaatt ttagtttcat attaaaatat
      181 acttttcata gacactacat tctttcatac tcattttcac atttaactat acaagtagtc
      241 ttactcattt atatcctatg ctttattaat atttattaag aaaaattcaa agatttaaaa
      301 atatatattt tctataaagt atatttatga attatttcat atcttctttt aaaataacat
      361 tactaacttc tctatgttca tttctatact tagttggaga tatattataa ttctttttaa
      421 ataagcgact aaaataattt atgctatcaa agccacatct catagcaatt tctgtcatat
      481 ttaaatctgt ttcctttaaa taatatatag ctttatcaag cctgattcca tttataaaat
      541 cgattgtagt cttacctgtt atttgcttaa atgttctaca aaagtgatgt gcactcaagt
      601 tcacacgact agcaagttct tttaatgtaa ttttatccat atatttttct tctatcaaat
      661 caaagataca ttcaaatttt tctaaagaat actttctttt tataaattca tcttcagaat
      721 atattttatc aatatgactt cttaataaga gtactattaa ttgatagata ttagctttta
      781 tagataactc atatccaact tctttattgt gatattcatc aagtatagtt gtaatacatt
      841 ttaaaatcag agaatcattt tgaattaaat tattaaatac aatatgattt aaagccaatg
      901 gtgctaagta cttggtttgt aacaaatcaa cttgattact aaaaaggaat gtgggttcaa
      961 tacgaatact atagaatttt aaattatctg ataagctttc taaataatgt aattcattac
     1021 tgtttgctat tacaatattt ccaggcacta ccttaaaatt atccaatcca cattcaagat
     1081 atgctatacc atcaataaaa taatatattt gtagatgctc atgccaatgt cttttaaagt
     1141 ttgctccttt tttaatgtca tcctttaaaa aaatatctat aaagttttct tgacctttcc
     1201 tgttaaaata tgtattttta tccttcatta ttcccccctc atcaaaatag tgctaattat
     1261 aagcaatata gttattgtat tcattatttc atatcgctat tatataaaat gtaactatca
     1321 ttgtaaacat taacataatg cagtcattat aataaggagg taataaaaat gacaaactct
     1381 tttaaaaaag atgataatag aaaaaaaatc acagttttat ttgtagtcat tgccatgact
     1441 tttatggcaa cattagattc tagtattata aatgttgctc tcccagttct tgcaagcaaa
     1501 ctaaatgtat ctctagcatc tattgaatgg gtaatagcaa gttactcaat tattatttgt
     1561 tcaacactat tattttttgg aagacttgga gatattattg ctaaatcaag agtatttcaa
     1621 gtaggtacta ttctctttac atcagcttca ttactatgtg gattaagtaa ttctttaact
     1681 ttacttatag tttgccgctt tattcaagga ataggcgctt ctgcctacat ggcaaacaat
     1741 catggaatta tcacagaact atttccaaaa gaaagcagag gaaaagccct agggatttta
     1801 gttactgcag ttgctattgg aaatatggtt ggaccatctg tgggaggatt tattttgtca
     1861 atatttaatt ggaatgttat attttttata aatattccaa tcggtcttat tgtaatcttt
     1921 ttaaatacaa aatttcttcc taatagtaaa aaatcttcag aaaatatgga taaaactggt
     1981 gctatacttc aatttttagg aacaacatta ttttttagtg ctctaattag tgctcaacaa
     2041 acaggattac taaatccata tatattaatt gcactattat tatcaattat ttttattata
     2101 ttatttttga tattagaaaa aaaacatcct caaccgcttc ttgaccttga aatttttaga
     2161 aattttaaat tttcattgaa tttaatttgt gctcttacat cattcatttg tattgcatca
     2221 tcaagtattt taattccatt ttatttacaa agtactatga aacttccccc tattcaagca
     2281 ggtttattta tgatactatc acctttgatt ttagccattt tttcacctat atttgggaat
     2341 atatcagata aaattaagtc agaaaaaatt attttaattg gtcttctcgt tatgagtttt
     2401 ggattctttc taatgtcaag attgaaagaa agttctgccc ttattttatt tgtaatttat
     2461 atatcgataa tatcaatagg acaagctata tttcaacctg ctaacaatgc tttgataatg
     2521 tcaagctgtt ctagaagtaa gctaggagtt gtaggaagca taaattccct tgtaagaaat
     2581 ttaggacaag ttataggtat tactatttct acaacacttt tatataattt tatgagtatt
     2641 aaagctggat atagggtcaa tgattatgtg attaataatg ataaaatatt tgtatttgga
     2701 atgagaaatg tttatattat tgttacactt gtttgcttaa taggtgcaat attaattggc
     2761 ttttatttat ttaaatataa taaaaatgaa caatagtttt tataattata ttcaaattat
     2821 gattatatta taagaaattt actaaaatac ttcctttgag ttaaatttaa tttggacata
     2881 ttgacttaaa aatgttttat taatttgata aaatatttct tttcctatag tttataaata
     2941 aaaatcagct gagcaaaata tttagtatat tatgctcaac tgattctttt attatttaac
     3001 taaaacttca atattttcat cttttccatc tataactata gtacatccat tatacacatc
     3061 tccagcaata agtttctttg caattatagt ttctaatgta tttcctatat atctcttaag
     3121 aggtcttgct ccatatacag ggtcatatcc ttctctcacc ataacatctt ttgctgaatt
     3181 tgtaacttca atagtaatat ctttttcttt taatctattt tttaaatctt tcataaatat
     3241 atctataatt ttcttgatat tttcttggtc taaaggctta aacattataa tatcatctac
     3301 tctgtttaaa aactcaggtt taaatctatg cttcatttca ttcattacta aattgttagt
     3361 agtctctgtt atatttcctc ctgcttctaa tagataatta cttcctatat tagaagtcat
     3421 tattataaga gtatttttaa agtctactgt cttaccttta ttatcagtaa gtctaccatc
     3481 atctaatatc tgtaagaaca tgttaaatac atcctcatgt gctttttcta tctcatcaaa
     3541 tagtattaca gaataaggag cacgtctaac tgcttctgta agttgaccac cctcttcata
     3601 tcccacgtac cctggaggag gtccaactag tcttgataca gcatgttttt ccatgtattc
     3661 agacatatct attcttatta tattatcttc actatcgaat aaatttctag caagagtttt
     3721 tgcaagttca gttttaccaa cacctgtagg gcctaagaaa ataaatgaac caattggttt
     3781 tctttcatct tttaatcctg ctcttgcacg tattactgca tttgaaactg ctgtaactgc
     3841 ttcatcttgt ccaataactc ttttatgaag ttcatcttct agtttaagta atttttctct
     3901 ttctccttca actagtttag ttactggtat acctgtccac tttgatacaa tgctagatat
     3961 ttcttcttca gtaacttctt cttttaaaag agaattttca gaaccatcat tcatattttc
     4021 ttcgtactgc tttatttgtt cttctaactt aggtatttca ccatatttaa cctctgctgc
     4081 tttgttgaaa tcatattctc tttcatactt ttcagcttta cctttagctt cgtctaattg
     4141 agcctttaag tttttaatat ctaatatttg actcttttct ttttcatatt ttgctgtcat
     4201 ttcatcattt ttagatttta actctgctat ttctttttga atatcatcca atcttgattt
     4261 acttttatca tcattttctt taagtaatgc ttctctttca gtttctaatg taaataactt
     4321 tcttctaaca acatctaatt ctgttggcaa tgaatctatt tcactacgaa tcatagctcc
     4381 agcttcatca attaaatcaa ttgctttatc tggtaaaaat ctatcttgta tatatctatg
     4441 agaaagtttt gctgctgcta caatagcatt atcatgtatt cttactccat gatgtatttc
     4501 aaatctttct tttaaacctc taagtataga tattgtatct tcaacagtag gttcttctgc
     4561 aattacaggc tgaaaacgtc tttcaagagc cttatctttt tctatatact ttctatattc
     4621 atcaaaagta gttgcaccaa tacagttcaa ttcacctctt gcaagcatag gtttaataag
     4681 attacctgca tccattgaac cttctgtttt accagctcca acaatagtat gaatctcatc
     4741 tataaacagt ataatttttc catcagaact ttgaacttct tttaaaactg cctttaatct
     4801 ttcttcaaat tcacctctat attttgcacc agcaattaaa gctcccatat caagtgagaa
     4861 tacaatctta tcttttaaac cttctgggac atctcctctt actattctct ctgctaaccc
     4921 ttctacaatt gcagttttac ctacacctgg ctcaccaatc aatacaggat tattttttgt
     4981 tcttcttgat aaaattctta taatccttct tatttcttca tctctcccaa ttacagggtc
     5041 taatttattt ttcttggcta aatctactaa gtttgttcca tatctagcaa gtgcatcata
     5101 tgtgccttct gggtcttgtg tttctactct ttgactacct ctcacttgtg ataaaatatt
     5161 taaaaagtca ttcttattta tattatattg ttttaatatt ttaccaacag ctgattttga
     5221 ttctgtttcc atcattgcaa gcataacatg ttctacactt atatatgaat ctttaaattc
     5281 tttagaaata ctctctgcct ttataagcac ctcatttatt tttctagtag ctgatactcc
     5341 ttgtgagtca gcaccttctc catatacttt tggaagctta tcaatttcaa agtttacagt
     5401 atttctcaaa gaatcaattg atatattcat tttttcgatt atatttggta tcaatccatc
     5461 ctcttgatta ataagtgctg agaaaagatg aattacatct acttgttggt tatgattttt
     5521 tactgcttcg ttataagcct catttaaact tttttgaact cttaaagtca ttttttctac
     5581 gtccatataa atccctccat tttaatatta tatccaaata aattaaattc tctcatttat
     5641 aattattgat tttctaagct ctaaaagact atcaagtaaa ttctcgtttt tttgtatatc
     5701 gaaactcaat gttaaattaa atgttccatc tgaattaaaa aaatcgaata cttcttttga
     5761 ttttgctatt aaatacaagt cttcatctat aagtcttttt tcacctatta ataaatactg
     5821 acatttatct ataaaactct ttttaaaatc aatagtgtat agattttcta aaatattata
     5881 ttgataaata gcaatattat taaacacttt taatatgttt gaatttacaa aattgtaaat
     5941 ttgattattg ttatacagtg atatttgttc gtatatatat tccctaaata aaggatgaat
     6001 gttttgctta agttttaaag taacttcagc tggtatttca aaataaagct tccagtactt
     6061 agagttattt ttttctaaaa taaaagctac tttgttcaaa ctggtagaac taatctcctt
     6121 tagtgtacaa ttaaaattat agtgtctcaa actagttttt tcattctcaa atacatagtc
     6181 aaaccactgt agaacatttc tatcaagttt taaactcata taaaaacctt cttccttatt
     6241 tactaacctt attaggtctt tttatctgat tatattctgc taataaactt tctttatcag
     6301 ctctactacc tttatatgct atatgtggat ttatcatata ctcaccattt ttcactcttt
     6361 gtaaaaaatc catttttttc agctccgtaa acaatgaaga taaagaagtt tttggatatt
     6421 caaattcaca agataattca tataatgttt ttctaatctt attattttta tccatagagt
     6481 ttattaattt taatggaatt aaatccctct tagtatacat tccttctact actttaatat
     6541 aattcttaaa acaaacattc atatacttac ttttattcat aactaaactt ttgctctctg
     6601 tatattcctg atttaaatcc ttttttaatg tcttatccaa attaaatatc tcttctcact
     6661 ctccttatct ctaattgata atataaatat attacaaaaa aatatgtatg tcaaaactga
     6721 aaatgcaaaa agttcacctt ataagtaaac ttttatttag ctataagcta aactttatta
     6781 tttttttaat ttagtatttc caatttattg gcaggatttt tgaacaatat ttataaattt
     6841 tttaaaaaaa tttaaatcta tttaatatgg cagactttca taaaatacat tacatttatt
     6901 aaatgtataa tattggcaat tagttttttc taaaatcagt tgacatatta tatatatatt
     6961 tatataatgt gtatatacta tatatacaat ttaaggaggt gtcttattga atatagttat
     7021 tagtaattcg agtgggaagc caatatatga acagattact acacaaatta aaagtatgat
     7081 tataagtgga gagttaccag aaggctcagc tcttccaagt atgagactat tggcaaaaga
     7141 acttagaata agtgtaatta ctacaaaacg agcatatagc gatttagaac gagacggatt
     7201 tattgagaca gttactggta aaggcagttt tgtatctagt aaaaacatgg attttgtcaa
     7261 agaagaacaa tttcgtttaa ttgaagaata cctacaaaaa gcagttgata cagcaaaaag
     7321 tagtgatatt acatatgacg aattaaaaga tattttattt ttattatata aaggagaatg
     7381 ataagtatgg atgcaataac tataaaaaat ttaaataaaa cttataaaga ttttagccta
     7441 caagatatat ctttttcagt accaaaaggg agtgtaatgg gatttgtagg tgaaaatggt
     7501 gcaggaaaaa caactacatt aaaagcaatt ttaaatttaa tatcttatga tagtggaaat
     7561 atagaaatct ttggtcttga tagtaaaaaa aatgaaaaag aaattaaaga gcaaattggg
     7621 gttgtatttg agggaagcaa ctttcatgaa aatttaaaca ctgaccatgt ttcaaaaatc
     7681 atgagtaaaa tctataaaaa ttggaatgac acattattta aagactactt aaaaaaattg
     7741 agagtaccag acaataaatt aattaaggag ttttcaaagg ggaataagat gaaacttagt
     7801 attgctgtag ccttatctca taaaccaaaa ttattaatac tagatgaggc aactagttca
     7861 cttgacccta tcgttcgtga agaaatacta gatatatttt tagattttat acaagatgaa
     7921 gaacactcta ttatcttatc ttcacatatt acaagtgatt tagataagat agcagactat
     7981 attacattta tacataaagg aaaaattgta tttagtgaaa ataaggatga attaattgat
     8041 actatgggag ttttaaaatg taaacctagt gattttgata acctttcaag agaggattat
     8101 tcatattatc gtaaaagcca atttggatat gaagtacttc ttaaagataa gcatagattt
     8161 atctcaagac atccaaactg tattgttgat aatacgtcta tagaagaaat tatgttattt
     8221 tatgtgagag gtgataaata atgaaaggtc taattttaaa agatttactt aatttgaaag
     8281 gaaatataaa gtttatactt ttattcatca ttatgtttgg ctttatgtct tctttaggag
     8341 atggaaatgt taataacttt ataggtgtta ttatagtttt atgtactaca atgattgttt
     8401 caacattttc atatgatgat ttgaataaat gggatagtta tgtacttaca atgcctatta
     8461 atcgca
//
LOCUS       DAEPIN010000110         8463 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.112, whole genome shotgun sequence.
ACCESSION   DAEPIN010000110 DAEPIN010000000
VERSION     DAEPIN010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8463
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.112"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            153..608
                     /locus_tag="KR250_003425"
     CDS             153..608
                     /locus_tag="KR250_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HBH0069311.1"
                     /translation="MDEIDLKIINSLKKNSRASTSEISRQINLSVPAVSERIRKIEQS
                     NLIQKYTIQINREKTKYKLLAFIFINTDLSKSMKEFKKTILEYDTVLECHHISGEYDY
                     LIKVLAEDTSNLEEFISVTLKEIGGIQKANTTIVLSTIKEEFNTIKDIF"
     gene            657..1241
                     /locus_tag="KR250_003426"
     CDS             657..1241
                     /locus_tag="KR250_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HBH0069312.1"
                     /translation="MKVLVFLAKGFETMEFSVFVDVMGWARNDYGHDIDVVTCGFKKQ
                     VMSTFNIQVLVDKTIEEVCVDDYDALAIPGGFEEFGFYDEAYDSSFLNLIREFNSKEK
                     IIASICVAALPVGKSGVLKNRKATTYHLKNGKRQRQLSEFDVNVVNEPIVVDKNIITS
                     YCPETAPHVAFKLLEMLTSKEQMDEVKLAMGFKL"
     gene            complement(1246..1569)
                     /locus_tag="KR250_003427"
     CDS             complement(1246..1569)
                     /locus_tag="KR250_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0069313.1"
                     /translation="MKKCLDNYSCPIEATLALIGGKYKTLILWHLKDTILRFNELKKL
                     IPKATPKMLTQQLRELESDGLIIRVVYPVVPPKVEYSLSDFGKSIIPILDSMCDWGSD
                     YLENL"
     gene            1721..2239
                     /locus_tag="KR250_003428"
     CDS             1721..2239
                     /locus_tag="KR250_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBH0069314.1"
                     /translation="MNFVELAKKRYSCRNYQDRKVEKEKLEKVLDVARIAPTGGNRQP
                     QRLIVIQEKEGINKLSKAANIYDAPLAILVCGDKDKVWTRPFDGKQLTDIDTSIVTDH
                     MMLQATELGLASVWVCYFNPDIIREEFSLPDNLEPINILLMGYESKIPESPERHEKTR
                     VPLSEIVSYETL"
     gene            complement(2415..3083)
                     /locus_tag="KR250_003429"
     CDS             complement(2415..3083)
                     /locus_tag="KR250_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HBH0069315.1"
                     /translation="MLEVSHKVKEIYPNIKFGVMVINITHSKPNKENFLVLKNSTIKN
                     IIEQHPEYNRKEKIKTEPVSSYIKYYKKFKKTYPVLLQLESILLKSKGIPDVGVTIES
                     MFLAELKNLLLTAGHDLDKMELPLKIELANGSEHFYGIGGKEHILTKDDLFLSDNIGI
                     LSSILNGPDNRTAITKDTKNIMYFVYGPDKISEKQIQDHLNDIKHYISSAFPDLKVNS
                     IDIF"
     gene            3198..4613
                     /locus_tag="KR250_003430"
     CDS             3198..4613
                     /locus_tag="KR250_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBH0069316.1"
                     /translation="MWITINRNSNISLSRQIYCEIKDMILDGSLKFEQKLPSSRTLSK
                     ELSVSRNTVLDAYNQLIAEGYLETRNGYGTIVAKGISGHKVDFKLNDTFRDEKDLHTN
                     KQKNYIDFRSGVPELKLFPRKELSKLYKQILYDLSDSDFRYGSTAGVWVLRVEISKYL
                     FRTRGIRCNPKNIMIVSGSTQGLSLISQLLYKSNKKVAVEDPIHKGLLKVISSIGYSV
                     VGIKVDDKGMNTNLIKSIKGISFVYTTPSHQYPLGGILPIQRRLELIKCATENDYYII
                     EDDYDSEFRYEGQPISSLYELSPNKVIYIGSFSKILSPAIRVGFMLLPDNILESYKTL
                     KMYSDVHTETILQHVLAEFIGNGGLEKYIFRMKKLYNRKRKYLIKVLNTEFKNEFKIK
                     GEAAGLHIVAHFYNVKFTESLIHKIFEAGIKVYPIQQYAIENILEYDYEIILGYAHLS
                     FDEIEKGVKILSKIIHMNQGT"
     gene            4836..5294
                     /locus_tag="KR250_003431"
     CDS             4836..5294
                     /locus_tag="KR250_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0069317.1"
                     /translation="MEIIDYIEENLTENLNLDKIAYIANYSKYHLHRMFTNVVGCTIH
                     QYIQRRRLTEAARQLIFTDKYIIDIAITVGYSTHQSFTLAFRNLYKESPQVYRNKDEF
                     YPIQQRFVLNLNHQIQSEKCISYANMNYKQRRDEINSYLNFNINCEVKVA"
     gene            5291..5875
                     /gene="clpP"
                     /locus_tag="KR250_003432"
     CDS             5291..5875
                     /gene="clpP"
                     /locus_tag="KR250_003432"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HBH0069318.1"
                     /translation="MSLVPHVIEQTGQGERSYDIYSKLLKDRIIFIGEEINDAIASLV
                     VAQLLFLESEDPDSDIIIYINSPGGSATSGFAIYDTMNYVRCDVSTICIGMAASMSAF
                     LLAGGTHGKRCALPNSEIMIHQPMGGAKGQATDVKIAVDNILKIKERLDKILSENTGK
                     SIEDIRRDTDRDNFMTALEAKEYGLIDYIMNRNE"
     gene            6187..7263
                     /locus_tag="KR250_003433"
     CDS             6187..7263
                     /locus_tag="KR250_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBH0069319.1"
                     /translation="MITISLCMIVKNEEEVIGRCLECVKDIVDEIIIVDTGSTDSTKE
                     IVSKYTDMVYDFEWIDDFSAARNFSFSKASKDYTMWLDADDIILEADREKLLKIKESL
                     DTSIDIVMAKYNVSFDENGNPTLSYYRERLFKRSSNHKWVDPIHEVVPLFGEVFYSDI
                     AISHKKLHRQDPLRNLRIFEKMISEGKTLEPRHQFYYSRELYYNARYKEAIEGFTKFL
                     DSSRGWIEDCISACRDLATCYYLINDEKSALYSLFRSFEFDEPRAEICCDIGKHMFDR
                     QKYKEAIFWYKVALTRDKNDTNGGFKSNDCYGYIPSIQLSVCYDRLGESDKAIYYHEK
                     TKEIKPNDSAVLHNENYFSKFKNS"
     gene            7330..>8463
                     /gene="bclA3"
                     /locus_tag="KR250_003434"
     CDS             7330..>8463
                     /gene="bclA3"
                     /locus_tag="KR250_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like exosporium glycoprotein BclA3"
                     /protein_id="HBH0069320.1"
                     /translation="MLLIMSRNKYFGPFDDNDYNNGYDKYDDCNNGRDDYNSCDCHHC
                     CPPSCVGPTGPMGPRGRTGPTGPTGPTGPGVGATGPTGPTGPTGPTGNTGNTGATGLR
                     GPTGATGATGPTGATGAIGFGVTGPTGPTGATGATGADGVTGPTGPTGATGADGITGP
                     TGATGATGFGVTGPTGPTGATGVGVTGATGLIGPTGATGTPGATGPTGAIGATGIGIT
                     GPTGATGATGADGATGVTGPTGPTGATGADGVTGPTGATGATGIGITGPTGPTGATGI
                     GITGATGLIGPTGATGTPGATGPTGATGPTGVGVTGATGATGATGADGATGVTGPTGA
                     TGATGANGLVGPTGATGAAGTPGATGPTGATGPTGVGITGATGA"
BASE COUNT         3225 a         1069 c         1537 g         2632 t
ORIGIN      
        1 tttaagtatt atatgtgttt tgattaaaga aaactaaaat tttaaggaaa aataacatat
       61 ttgtattaaa ttttattgaa atgattaaat ttatttactt aatgtaaatt attagctaaa
      121 attgtattaa ataattaagg aggtgagtag ctctggatga aatagattta aaaataatta
      181 attctctaaa aaagaatagt agggcttcaa cctctgaaat aagcagacaa attaatttat
      241 cagtaccagc agtctcagag cgtataagga aaattgagca gtcaaattta attcaaaagt
      301 acactattca aattaatcgg gaaaaaacaa aatataagct tttggctttt atatttatca
      361 atactgattt atctaaaagt atgaaagaat ttaagaaaac tatattggaa tatgatactg
      421 tattagaatg tcatcatatt tcaggagagt atgattactt aataaaagta ttagcagaag
      481 atacaagtaa tcttgaagag tttatatcag tgactttgaa ggaaattggg gggattcaaa
      541 aagctaatac aaccattgtt ttatctacaa ttaaagaaga atttaataca ataaaagata
      601 ttttttaaat aatttactag taaagtgtta attatatttt aggaggaaaa tagaatatga
      661 aagttttagt ttttttagca aaaggttttg aaactatgga atttagtgta tttgtagatg
      721 ttatgggatg ggctcgtaac gattatgggc atgatattga tgtagtgact tgtggattta
      781 aaaaacaagt tatgagcaca tttaatatac aagtattagt tgataagacg atagaagaag
      841 tatgtgttga tgattatgat gctctagcta ttcctggagg atttgaagaa tttggatttt
      901 atgatgaagc atatgattca tcttttttaa atctaataag ggagtttaat tcaaaagaaa
      961 aaataatcgc ttctatttgt gtggcagcat tgccagttgg aaaaagtggt gttttaaaaa
     1021 atcgtaaagc tactacatat catttgaaaa atggtaaaag acaaagacag ttaagtgagt
     1081 ttgatgtaaa tgtagttaat gaaccaattg ttgtagataa aaatattata acctcatatt
     1141 gtccagaaac agctccacat gtagctttta agcttttaga aatgttgaca tctaaagaac
     1201 aaatggatga agttaaatta gcaatgggat ttaaactgta atggtttata gattttctaa
     1261 gtaatcagaa ccccagtcac acatggaatc taaaattgga ataatactct ttccaaaatc
     1321 agatagcgag tattctactt ttggaggaac aacaggataa accactctta taattaaacc
     1381 atctgattct aattcacgta attgttgagt taacatctta ggcgttgctt taggaattaa
     1441 tttttttaat tcattgaacc tcaatatagt gtctttaaga tgccaaagta taagtgtctt
     1501 atatttacca ccaatcaatg caagtgttgc ttctattggg caactgtaat tatctaaaca
     1561 ttttttcatt tttgggctcc tttatttatt ttaaagtatc tttttaggta ctatattaca
     1621 aaaaagtgca ttattgtaat atagtaccat agtgataaaa ttatagcata ggttaaataa
     1681 atcatcaact tagcctaata aaacaatagg gggtaaataa atgaatttcg ttgaacttgc
     1741 aaaaaaaaga tattcttgcc gtaattatca agatagaaag gttgaaaaag aaaaacttga
     1801 gaaggtttta gatgttgcaa gaatagctcc aactggtggt aatcgtcaac ctcaaagatt
     1861 gattgttatt caggaaaaag aaggtataaa taaactttct aaggctgcca atatatatga
     1921 tgcgccatta gcaattcttg tatgtggaga taaagacaaa gtttggacaa gaccttttga
     1981 tgggaaacaa cttacagata ttgacacaag tatagttaca gaccatatga tgttacaggc
     2041 aactgaactt ggattggcaa gtgtttgggt gtgctatttt aatccagata taattagaga
     2101 agagtttagt ttgccagata atctagaacc aataaatata ttattgatgg gatatgaatc
     2161 taagatacca gagagcccag agagacatga aaaaactcgt gttcctttaa gtgaaattgt
     2221 ttcttatgaa actctttaat tataaaatta agttattcat aggataaatg taataaagtg
     2281 tttgttataa acaaaattca aaataaataa tatctacata taattaattt tagtgaagta
     2341 tatctagttt aactagaatg tatacttcac taatttattt attcaaaata tttatgagga
     2401 ttttaatata ttaactaaaa tatatcaatt gaattaactt ttaaatctgg aaaagcactt
     2461 gaaatatagt gttttatatc atttaaatgg tcttgaattt gcttttctga tattttatca
     2521 ggtccatata caaaatacat aatattttta gtatcttttg ttatagctgt tcgattatca
     2581 gggccattaa gaatactaga taatatacca atattatctg ataaaaataa atcatctttt
     2641 gttaaaatat gttctttacc acctatacca taaaaatgtt cacttccatt tgctaattct
     2701 attttcaaag gtaattccat tttatcaagg tcatgacctg ctgttagaag aagatttttc
     2761 aattctgcta aaaacattga ctcaattgtt acacctacat ctgggatacc ttttgatttt
     2821 aatagaatag attctaattg aagcaataca gggtaagttt tcttgaattt tttataatac
     2881 tttatataac ttgaaacagg ctctgtttta attttttctt tgcgattgta ttctgggtgt
     2941 tgttctataa tatttttaat tgtagagttt ttaagaacta aaaaattttc tttattaggt
     3001 tttgaatgtg taatatttat aaccataact ccaaatttaa tattgggata aatttcttta
     3061 actttatgtg atacctctaa cataatatag actccttttt attttatatt ttatgatata
     3121 atcttagcaa attaaaagtg gattttatag ttccattttt atagtaattt tatagaacca
     3181 cttttggagg tattagaatg tggattacga ttaatagaaa tagtaatata tcattatcaa
     3241 gacaaattta ttgtgagata aaagatatga ttctggatgg aagcttaaag tttgagcaaa
     3301 aactgccatc atctaggact ttatcaaaag aactatctgt atctcgtaat acagttttag
     3361 atgcatataa tcaattaatt gcagaaggat atttggaaac aaggaatgga tatggaacaa
     3421 ttgtagctaa aggaatatca gggcataaag ttgattttaa gttaaatgat acttttagag
     3481 atgaaaaaga tttacatact aataaacaaa aaaattatat agattttcgt tcaggagttc
     3541 cagaattaaa actatttcca cgcaaagaac tttcaaagct atataaacaa atactgtatg
     3601 atttatcaga ttcggatttc agatatggaa gtacagctgg tgtatgggtt ttaagagtgg
     3661 aaatatctaa gtatttattt cgtactagag gaattagatg taatcccaaa aacattatga
     3721 ttgtttctgg ttcaacacaa ggtttatctt taatttcaca gcttttatac aaaagtaata
     3781 aaaaggttgc tgtagaagac cctattcata aaggcttatt aaaggttatt tcatctattg
     3841 gatattcagt tgttggaatt aaagtagatg ataagggaat gaataccaac ttaataaaat
     3901 caataaaggg gatttctttt gtttatacta caccttctca tcaataccca ttaggaggga
     3961 ttttacctat tcaaagaaga ctagagctta ttaaatgtgc aacagagaat gattactata
     4021 taattgaaga tgattatgat agtgaattta gatatgaggg tcaaccaatt agttctcttt
     4081 atgaacttag cccaaataaa gtaatttaca taggttcttt tagtaaaata ttatctccag
     4141 ctattcgtgt tggatttatg ttattaccag acaatatttt agaaagctat aaaaccttaa
     4201 aaatgtactc tgatgttcat acagaaacaa tattgcaaca tgtattagct gaatttattg
     4261 gaaatggagg tttagagaag tacatattta gaatgaagaa gttgtacaat agaaaacgta
     4321 agtacctcat aaaagtttta aacacggaat ttaaaaatga atttaaaata aaaggtgaag
     4381 cagcagggct tcatatagtt gctcatttct ataatgttaa atttacggaa agtttaattc
     4441 ataaaatttt tgaagcaggt ataaaagttt atcctataca acaatatgca attgagaata
     4501 tattagaata tgactatgaa attatactag gttatgctca tcttagtttt gatgaaattg
     4561 aaaaaggtgt aaaaatactc agcaaaataa tacatatgaa tcaaggtaca tagataaaac
     4621 atgtagcaat ctacatataa tagatgtaat taagtttaaa ctttaaaaga aatatatatt
     4681 aatatttttg tataaaaata cctgatattt ttttatcgat actttattgc tatacactaa
     4741 attaagatag caatttatat taagaaaaaa ctttttgcat aggttataat acttaaaagg
     4801 agaggataga gttgaaaaca aatacagatg ttattatgga gataattgat tatatagaag
     4861 aaaatcttac agaaaatcta aatttagata aaatagctta tattgctaat tattcaaaat
     4921 accatttaca cagaatgttt acaaatgttg taggatgtac tatccatcaa tacatacaga
     4981 ggaggagact tactgaagca gctagacaac ttatatttac agacaaatat attatagaca
     5041 tagctattac agtaggatat tctacacatc aatcttttac actagcattt agaaatttat
     5101 ataaagaatc tccacaagta tatagaaata aagatgaatt ttatcctatt caacagagat
     5161 ttgttttaaa tctaaatcat caaattcaaa gtgaaaaatg tatttcttat gcaaatatga
     5221 attataaaca aagaagagat gaaatcaatt cttatcttaa ctttaatatt aattgtgagg
     5281 tgaaagtagc atgagtttag tgccacatgt aattgaacaa acaggtcaag gggaaaggtc
     5341 ttatgatata tattcaaaat tattaaaaga tagaattata tttataggag aagaaattaa
     5401 tgatgctatt gcaagtttag tagtagctca attactgttt ttagaatcag aagacccaga
     5461 ttcagatata attatatata taaatagccc tggtggctct gctacatcag gatttgctat
     5521 atatgatact atgaattatg tcagatgtga tgtatcgact atttgtattg gtatggctgc
     5581 aagcatgagt gcttttttac ttgcaggagg aacacatgga aaaagatgtg ctttacctaa
     5641 ttcagagatt atgattcatc agccaatggg aggagctaaa ggtcaagcaa cagatgtaaa
     5701 aatagctgta gataatatat tgaaaataaa agaacgatta gataaaattc tttctgaaaa
     5761 tacaggtaaa tcaatagaag atattaggag agatacagat agagataatt ttatgactgc
     5821 actagaggct aaagagtacg ggctgattga ttatataatg aataggaatg aataaagtat
     5881 caaagtatca tattaaaatg tctgttttac aaatatatat ataatattaa ataagagtgt
     5941 ctcaaaatga actttttagt ttatgggaca ctcttttttg tatgaaatta aatatggttt
     6001 tatcatttca acaattatta ccaaatttca agtttaataa attctactat aaaaaataca
     6061 taaaagataa ggttctttag taactttctg tcgtcaaaag tttttataaa tttaataaca
     6121 aaacaagaga aaataccata tattacttat gtctgcaata tatagaagac aaaggagttg
     6181 tacattatga ttacaataag tttgtgcatg attgttaaga atgaggaaga agttataggc
     6241 agatgcttag aatgtgtaaa ggatatagta gacgaaatta taattgtaga tacaggttct
     6301 acagattcca caaaagaaat agtctcaaaa tatacagaca tggtgtacga tttcgaatgg
     6361 atagatgatt tctctgctgc aagaaatttt tcattttcta aagcttctaa agactatact
     6421 atgtggcttg atgcagatga cataatttta gaagctgata gagaaaaatt attaaagatt
     6481 aaagaaagct tggatacttc cattgatatt gttatggcaa aatacaatgt atcctttgat
     6541 gaaaatggta acccaactct ctcgtattat agagaacgat tgtttaaaag aagttctaat
     6601 cataaatggg ttgaccctat tcatgaagta gtacctcttt ttggagaggt attttattct
     6661 gacattgcaa tatctcataa aaagcttcat agacaggacc ctctaagaaa tcttagaatt
     6721 tttgaaaaaa tgatttcaga aggaaaaaca ttggagccaa gacaccaatt ctattattct
     6781 agagagttgt actataatgc taggtataaa gaagcaatag aaggttttac taagttttta
     6841 gactcatcaa gaggatggat tgaggattgt atcagtgctt gcagagattt agccacatgt
     6901 tattacttaa ttaatgatga aaaaagtgcc ttatattcat tgtttagaag tttcgaattt
     6961 gatgagccaa gagcagaaat ttgttgtgac ataggaaagc atatgtttga tagacaaaaa
     7021 tataaagaag ccattttttg gtataaggta gcattaacaa gagataagaa tgatactaat
     7081 ggaggtttta agtcaaatga ttgttatgga tatataccat ctatacaatt aagtgtctgt
     7141 tatgacaggt taggagaatc agataaagct atttattatc atgaaaaaac aaaagaaatc
     7201 aaacctaacg atagtgctgt tttgcacaat gaaaattatt ttagtaaatt taaaaatagt
     7261 tgataggtca caatcatact aaatttgaca tattatatat agagcttaaa agctcaaata
     7321 tatcaggagg tgcttttaat aatgagtaga aataaatatt ttggaccatt tgatgataat
     7381 gattacaaca atggctatga taaatatgat gattgtaaca atggtcgtga tgattataat
     7441 agctgtgatt gccatcattg ctgtccacca tcatgtgtag gtccaacagg cccaatgggt
     7501 ccaagaggta gaaccggccc aacaggtcca acgggtccaa caggtccagg agtaggggca
     7561 acaggcccaa caggaccaac cggtccgact ggtccaacag gaaatacagg gaatacagga
     7621 gcaacaggat taagaggtcc aacaggagca acaggggcaa caggcccaac aggagcgaca
     7681 ggagctatag ggtttggagt aacaggtcca acaggcccaa caggagcgac aggagcaaca
     7741 ggagcagatg gagtaacagg tccaacaggt ccaacgggag caacaggagc agatggaata
     7801 acaggtccaa caggagcaac aggggcaaca ggatttggag taacaggtcc aacaggccca
     7861 acaggagcaa caggagtagg agtaacagga gcaacaggat taataggtcc aacaggagcg
     7921 acaggaacac ctggagcaac aggtccaaca ggggcaatag gagcaacagg aataggaata
     7981 acaggtccaa caggagcaac aggagcaaca ggggcagatg gagcaacagg agtaacaggc
     8041 ccaacaggcc caacaggggc aacaggagca gatggagtaa caggcccaac aggagcaaca
     8101 ggagcaacag gaataggaat aacaggccca acaggtccaa caggagcaac aggaataggg
     8161 ataacagggg caacgggatt aataggtcca acaggggcaa caggaacacc tggagcaaca
     8221 ggtccaacag gagcaacagg cccaacagga gtaggagtaa caggagcaac aggagcaaca
     8281 ggggcaacag gagcagacgg agcaacagga gtaacaggtc caacaggagc aacaggggca
     8341 acaggagcaa atggattagt aggcccaaca ggagccacag gagcagcagg aacacctgga
     8401 gcaacaggtc caacaggagc aacaggccca acaggagtag gaataacagg agcaacaggg
     8461 gca
//
LOCUS       DAEPIN010000111         8422 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.113, whole genome shotgun sequence.
ACCESSION   DAEPIN010000111 DAEPIN010000000
VERSION     DAEPIN010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8422
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.113"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            23..516
                     /locus_tag="KR250_003435"
                     /pseudo
     CDS             23..516
                     /locus_tag="KR250_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429361.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            1057..2412
                     /locus_tag="KR250_003436"
     CDS             1057..2412
                     /locus_tag="KR250_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBH0069321.1"
                     /translation="MDTKISINENMSLGKRFFKYLAPSVVAMWVFSLYTMVDGIFVSK
                     GVGELALAAVNISMPFINFIFAVSLLFSTGASTIIAIYLGKKDIKSANEVFSFNLVSI
                     IILSIIILAITFFNLDRLALFLGATESTIGMVKDYLGIIIFFNGFFIVSYSLEVIIKT
                     DGFPILATVGVIISALTNIILDYLFVIEFGWGVKGAGIATGLSQVFSTIFFLIHFLRK
                     NSTLNFSKFRIDFKTLRKIVFIGFPDSTTELSCGIVVLLFNLSLTKYIGENALIYYSV
                     INYINTLVLMTMMGITQGMQPLTSFYYGAGNIDNVKKLLKMGIKATIIASVAVFAICM
                     AFSGPIVSLFIHPEETMLFNEGVRVFKIFSISFLLVGINVIISGFFVSVEKPSISTVI
                     SLGRGLVIVVLSLISMILIFGGQGIWMTTIVSEFICLILSLVFLKKNFSTLDSNLNKV
                     A"
     gene            complement(2530..3198)
                     /locus_tag="KR250_003437"
     CDS             complement(2530..3198)
                     /locus_tag="KR250_003437"
                     /EC_number="6.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphthine--ammonia ligase"
                     /protein_id="HBH0069322.1"
                     /translation="MKEVLKLADEKFVMSFSGGKDSILALNRMLKKGYKPVALLTTIS
                     EEHGKSWTHNLEYNMLKQVSSNIGLPLLVAECGVEGYEESFERALIKAKNMGATICAY
                     GDIDIESHRKWDTDRCEAVGMKVDLPLWQESREDLVYEFIDSGFCSVVTKVNLKHLGE
                     EFLGKKLTRELVEKIKNAGADPCGEHGEYHTFVVDGPIFKKPVEYEVKGTLIKDGYGY
                     LEIK"
     gene            complement(3265..3693)
                     /locus_tag="KR250_003438"
     CDS             complement(3265..3693)
                     /locus_tag="KR250_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBH0069323.1"
                     /translation="MTKLWAIIFALLAGGSTALEAFINGELGKSTTALVATFISLTVG
                     AIFFLASIIVTGDLKCLVSINGFNYKLLLGGVFGGLIIYFTVKAIPNLGVSNTLILTL
                     VSQVLVGFFIDSVILGQETMHLYKYIGVILLILGTFFIVN"
     gene            3800..4393
                     /locus_tag="KR250_003439"
     CDS             3800..4393
                     /locus_tag="KR250_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HBH0069324.1"
                     /translation="MNFCYNCSIDFGGDFMYTNFNIDFNNFNKKENAIKFMLMGVLLI
                     ILGLLCLTFKTLGIKLISWTFGIALLFFAYLNLKNINELKRYATKEEIKPSINIQWVL
                     IIACILLFVFPQKIQSIFSLFLGFYLIFNQLVALVNSKNNPYSKFTTWNIVKILFGIC
                     LILSPLFLSRFIVSIMSFFIILFGLVLFFSGNTARKY"
     gene            4512..5447
                     /locus_tag="KR250_003440"
     CDS             4512..5447
                     /locus_tag="KR250_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893027.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HBH0069325.1"
                     /translation="MDKFKYAFDNKRYHTWNYYLRNTFGEKVFKVSINAGFTCPNIDG
                     SLGYGGCTYCSKEGSGDFAGNPKDNLISQFYNIKEMMLKKWPHAKYIGYFQAYTNTYA
                     PLEVLKEKYETILELEDVIGLSISTRPDCLPDDVVEYLSELNKRTNLWVELGLQTIHD
                     STSKIINRGHDYKTFVEGVEKLKSKNIKVVVHIINGLPGEDYNMMMETAKAVGKMGVD
                     GIKIHLLHVIKDTPMEKMLQNGMLTLMEQDEYINLVCDQLEILPETMIIHRLTGDGKR
                     DELVGPIWSLKKWEILNQIDDTLKARNSYQGCKFV"
     gene            complement(5718..6662)
                     /locus_tag="KR250_003441"
     CDS             complement(5718..6662)
                     /locus_tag="KR250_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HBH0069326.1"
                     /translation="MGEKSKGYVFIAIAGLLWATLGLFGKFLMGNGLTSEQVAFTRLF
                     FGFIVLGVYSSIRTPQILKISKKGIIYSVIIGIICQAMFNLCYFKAIDIAGVSIAAVL
                     LYTSPLFLAIFSKICYKENITRSKLFSLILCFIGAIMAVTGGRLDFQGLNAFGLLLGV
                     LSAIAYALMPTISKNALKEFSSSTILVYSFLFGAIFMIPSSRPWEILNYAKDLDVLSC
                     MLMLGIVPAALAYIFYAAGISKGVELSVAGVVASVELVGSVIIGCTILGESFSLGKLF
                     GVMLMLISAVVALNLSYDEIRIFYKSNKLKQIEKTESI"
     gene            6980..8410
                     /locus_tag="KR250_003442"
     CDS             6980..8410
                     /locus_tag="KR250_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBH0069327.1"
                     /translation="MNLDLEFYQKILEASHDEICVSDDKGIIIYCNKAFEENYGLKKE
                     DILGKNVSFLEDSGYSTKSPIPIVLKTKTKFSLEQDTQTGKKLIITATPIFDENGNLE
                     FTVENCRDITELNNIKNKLEDTKKQVKKYKSEVETLYRTALRIEDTVIMDGIVMRPII
                     NTVNHVSKTDVSVLLLGESGTGKSSLARYIHHNSNRSNGPFITINCATISPQLLESEL
                     FGYTSGAFTGASTKGKVGLVELANGGTLFLDEIGDIPQNLQAKFLQLIQDRTFTPVGS
                     LKNKKVDIRIISATNVDLVSKVKEKKFREDLYYRLNVIEIKLPPLRERRDNLVEIIKY
                     YFNRYSSDFNLNKTISKEAMDAIANYRFPGNIRELQNIIQKILLTCTDNHITIDDLPN
                     ILTKNIHITNNGNKTHISQINKVIISDSKSTNYKNKNFDTLIKEYEKNIILDAYEKFG
                     SSYKVAKHLEISQSKANRLIRKYTNT"
BASE COUNT         3124 a         1181 c         1077 g         3040 t
ORIGIN      
        1 aaataacata tacaatgagc acatgttaaa ttcaacaaaa aaccaagtcg attttataaa
       61 atcagtattg aaagaatctt atagattaaa agaaatatca gaaaatattt gtgattttgt
      121 tacaacccca atcaaaaaaa atagttcaac ttctaagctt tatacaaaac aaaaagctca
      181 attattatta gaaaaatcaa aaaatattcc tataggtata ccaatttttt tgatgctaac
      241 tcttggatta cgttttggtg aagctgtatc acttatatgg tcagatgtag accttgataa
      301 aaaatctcac atgtaaatca aacattagtt tatgtataca ataaaataat cttcaaaggc
      361 cctaaaaccc ctagattcaa aagaagatta tttgttcctg atgaacttat aagtttgttt
      421 aaaggggaaa aattaaaaca aaataagtta aaattacagg gtatattgaa aaatgaattt
      481 gatttgatat gcttaaataa aagattcaac ccgtgagttc aaccaacttt tgttgttcat
      541 tttaaaaaac tattaagaga taataatcct agagatataa aagtacatga attaatacat
      601 gcaaatgcga ctttgatatt actttcaggt actaacataa aagctattta taaaatatta
      661 gtacatgcag atataaaaat agctatgaat atatattcca tattctcact tattagatga
      721 aatgaataaa gaaacatctg aaaatataag caaaatacta ttcaaataat gttcagtttt
      781 atgtcaatta aatgtcactc aaagtgcttt ttttccattt gtcagttaaa tgtcacttaa
      841 agtacctttt tcctatttgt cagttaaatg tcagtaatat ataaataaga tatatttata
      901 agacccttaa aatcactatt atttaataaa atttataatt atatactatt tgctgttgac
      961 attggtatta taccatagtt tatcatttat attagaataa aattattaat taatagtttc
     1021 gtaattttat aatagattta cagggagtga taataaatgg atacaaaaat atctataaac
     1081 gaaaacatga gtctaggtaa aagatttttt aaatacctag caccatctgt agttgcaatg
     1141 tgggttttct cactatatac aatggttgat ggaatatttg taagtaaagg agttggagaa
     1201 ttagctttag ctgctgtcaa tatatcaatg ccttttatta attttatatt tgcagtttct
     1261 ctattatttt caactggtgc atctaccata attgcaatct atctggggaa aaaagatatt
     1321 aaatctgcta acgaagtctt ttcatttaac ttagtatcaa taattatatt atcaataatt
     1381 atattagcta taactttttt taatttagat agattagcac tatttttagg agctacagaa
     1441 tctacaattg gtatggttaa agattattta ggaataataa tattttttaa cggattcttt
     1501 atagtgtctt attctttaga agttataata aaaactgatg gctttccaat attagctact
     1561 gttggtgtaa taatatctgc tcttacaaac atcatacttg attacttgtt tgtaattgaa
     1621 tttggatggg gtgtaaaagg tgctggtatt gcaactggtt tgtctcaagt cttttctact
     1681 attttcttct taatacattt tttaagaaaa aactctactt tgaatttttc aaaatttaga
     1741 attgatttta agacacttag aaaaattgta tttataggtt ttcctgattc aactactgaa
     1801 ttaagttgtg gtatagttgt tttacttttt aacttaagtc ttactaaata tattggagaa
     1861 aatgctctta tttattatag tgtcataaat tatataaata cattagtact tatgactatg
     1921 atgggtataa ctcaaggtat gcaaccactt actagctttt attatggtgc aggaaatata
     1981 gataatgtaa aaaaattatt aaaaatgggg ataaaagcca caataatagc atcagttgct
     2041 gtatttgcaa tttgtatggc tttttcagga ccaatagtct ctttatttat acatccagaa
     2101 gaaacaatgc tattcaatga aggagttcgt gtatttaaaa tattctctat atctttctta
     2161 ttggtaggaa ttaatgtaat aatttctgga ttctttgtat cagtagaaaa accatcaatt
     2221 tctactgtta tatctcttgg tagagggctt gtaatagttg ttttaagtct aatttcaatg
     2281 atactaatat ttggtggaca aggaatatgg atgactacta ttgtttctga gtttatatgt
     2341 cttattttat cattagtttt tctaaaaaag aatttttcta cattagactc taatctaaat
     2401 aaggttgctt agatgataag agtgacaata tatgaaaata tctaaataaa aaatacctat
     2461 tatacaatct tgcaaatatt aaacctttgc aaaaattagg attatataat aggtatttta
     2521 tttatgtaat tattttattt ctaaatatcc atagccatct tttattaaag ttccttttac
     2581 ttcatattct acaggttttt taaatattgg accatctacg acaaatgtat gatactctcc
     2641 atgttctcca catgggtctg caccagcatt ttttatcttc tccacaagtt ctctagttaa
     2701 tttttttcct aaaaattcct ctcctaagtg ctttaagttt acttttgtta caacactaca
     2761 aaatccactg tctataaatt catatactaa atcctctcta ctctcttgcc ataatggtaa
     2821 atctactttc ataccaactg cttcacatct atcagtatcc cattttctat gactttctat
     2881 atctatatct ccatatgcac atatcgttgc tcccatgttt ttagctttta ttaaagccct
     2941 ctcaaagctc tcttcatatc cttctacgcc acactcagca actaataaag gtaatcctat
     3001 gttgctactt acttgcttaa gcatattata ttctaaatta tgtgtccatg actttccatg
     3061 ttcttcactt atagttgtca agagtgccac aggcttatat ccttttttca acattcgatt
     3121 taatgctaat atactatcct taccaccact aaatgacatt acaaattttt catcagccaa
     3181 ttttaacacc tctttcatct aactttgttt tattaacata attataccaa atttttgagt
     3241 tgcatagcaa attatattaa attgttaatt cactataaag aatgtcccta atattaaaag
     3301 tataacccct atgtatttgt ataaatgcat tgtttcttgt cccaaaatta cgctatcaat
     3361 aaaaaatcca actaaaactt gggatacaag agttaatata agtgtatttg atactcctaa
     3421 atttggaatt gcctttactg taaaatatat tattagacct ccaaatactc ctcctaataa
     3481 caacttataa ttaaaaccat ttatagatac tagacacttt aagtcacctg taactattat
     3541 acttgccaaa aaaaatattg ctcctacagt taagcttata aaggtggcca ctaatgctgt
     3601 tgtacttttt cctagctctc catttataaa agcttcaaga gctgtagaac cacctgctaa
     3661 taatgcaaat attattgccc ataattttgt catggacaaa cctccttcta tataattcta
     3721 ttaatagaag tttatgatac aacctattaa ttaattacta agtatagttt aatttttata
     3781 cactaggctt tatttggata tgaatttctg ttataattgt tctatcgatt ttggaggtga
     3841 ttttatgtac acaaatttta atatcgattt taataacttt aataaaaaag aaaatgctat
     3901 taaatttatg ttaatgggag tattactcat tatattagga ttattatgcc ttacatttaa
     3961 gacattaggt ataaaattaa tttcttggac ttttggtatt gcacttttat tctttgcata
     4021 tttaaattta aaaaatatta atgagttaaa aagatatgct actaaagaag aaattaaacc
     4081 ctctattaat atccaatggg ttctaataat tgcatgtata ttactttttg tatttcctca
     4141 aaaaatacaa agcatatttt cattattttt aggattttat ttaatcttta atcaactagt
     4201 agcacttgta aatagtaaga ataatcctta ttctaaattt acaacttgga atatagttaa
     4261 gattctattt ggtatatgcc ttatattatc tccacttttt ttatctagat ttatagtatc
     4321 gataatgtct tttttcataa tattatttgg actagttcta tttttctctg gaaatacagc
     4381 gagaaaatat taattatttt ctcgctgtat ttttatatta cttaaaatag aatttgttta
     4441 agttatctat ttgtggtaat atgttttttg tacaagctca gcgtacaatt ttattagaaa
     4501 taggtgattt tttggataaa tttaaatatg cttttgataa taaaagatat catacttgga
     4561 attactatct tagaaatact tttggagaaa aagtttttaa ggtatctata aatgcaggat
     4621 ttacttgccc aaacattgat ggaagtcttg gttatggcgg atgtacctat tgtagcaaag
     4681 aaggctcagg tgattttgct ggaaacccta aagataatct tatcagtcaa ttttataata
     4741 taaaagaaat gatgttaaaa aaatggcctc atgcaaagta tattggctac tttcaagcct
     4801 atacaaatac gtatgctcct cttgaagttt taaaagaaaa atatgagact atattagaac
     4861 ttgaagatgt cataggtctt tcaatatcca caagacctga ctgtttgcca gatgatgttg
     4921 tagaatactt atctgaacta aataaaagaa ccaatttatg ggttgaactt ggacttcaaa
     4981 caattcatga ttctacatct aaaattataa atagaggtca tgattataaa acatttgttg
     5041 aaggtgttga aaaactaaag tctaaaaata taaaggttgt cgtacacatt ataaatggtc
     5101 ttcctggtga agattataat atgatgatgg aaacagcaaa ggcagttggt aaaatgggtg
     5161 tagacggtat aaaaatccat ctattgcatg ttataaaaga tactcctatg gaaaagatgt
     5221 tacaaaatgg tatgttaact cttatggaac aagatgaata tatcaatctt gtctgtgacc
     5281 aattagaaat acttcctgaa actatgataa tccatagact cactggtgat ggtaagaggg
     5341 atgaactagt aggtcctata tggagcctaa aaaaatggga aattttaaat caaattgatg
     5401 atactttaaa ggctagaaat tcttatcagg gctgtaagtt tgtttaatac aaaataataa
     5461 ctatagaaat ggttatataa atatagtcct ttctatagtt atttagtatt tacaaaacaa
     5521 aatattaatt tgtacattta tacaatatat aataaaaaat agatagtcta aactgtatct
     5581 acttatattt tatttgtact gtccacttta ttctataaaa tttataaata gttatttttt
     5641 aataccaaat ataatttggt ataatttttg ttggactcta aaatgtatac caataaatcc
     5701 tttttaagta tataattcta aatactttca gttttttcaa tctgctttaa cttgttggac
     5761 ttataaaata ttcttatttc atcataagaa agattaagtg ctactactgc agatattaac
     5821 atcaacatca ctccaaataa cttccctaag gaaaaacttt ctcctaatat agtgcaacca
     5881 attattactg aaccaactaa ttctactgat gccacaacac ctgcaactga caactctaca
     5941 cctttagata tacctgctgc ataaaaaata tatgcaagtg cagctggaac aatgcctaac
     6001 attagcatac aagataaaac atctaaatcc ttagcataat ttaagatttc ccaaggtctt
     6061 gaagaaggaa tcataaatat agctccaaat aagaaactgt atactaatat tgttgaactt
     6121 gaaaattcct ttaaagcatt tttactaata gttggcataa gtgcatatgc tattgctgat
     6181 aataccccaa gtaaaagtcc aaaagcgttt aaaccttgaa aatccaaacg tccaccagtc
     6241 actgccataa ttgcaccaat aaaacaaaga ataagtgaaa ataatttgct tctagtaata
     6301 ttttctttat aacatatttt tgaaaatatt gctaaaaaca gtggtgatgt atatagtaaa
     6361 actgctgcaa ttgatacacc cgcaatgtca attgctttaa aataacataa attaaacatt
     6421 gcctgacata taatacctat tattactgaa tatataattc cttttttgct tatctttaag
     6481 atttgtggtg tccttataga tgaatatacg cctaaaacta taaatccaaa aaacaatctt
     6541 gtaaatgcta cttgttctga tgtcagtcca tttcccatta aaaactttcc aaataatcca
     6601 agtgtagccc ataataaccc tgctattgct ataaatacat accctttact tttttctccc
     6661 attttaaatt cccttctatt tttatttagt caatttacac tcttcttttt aagcaaaaac
     6721 tatgccatct taaatcaata tatatcgact ctaaaaccct agtctttcca ataatactaa
     6781 taaattttca atttttttat atttattttt cacttacttt aagttgattt agacttattt
     6841 taattgtaaa atgagttata tataacttat ttaaagttaa aatagactga aacttatata
     6901 taaatatcaa attttaagta tattaatcaa ttaaacctat tttaatatat cattaaaaat
     6961 ttaggaggaa aatataaaaa tgaatttaga tttggaattt tatcaaaaaa tattagaagc
     7021 ttcccatgat gaaatatgtg tttctgatga taaaggaata ataatatatt gtaataaagc
     7081 atttgaagaa aattatggtc taaaaaaaga agatatttta ggaaaaaatg tttctttttt
     7141 agaagactct ggatattcaa caaaaagccc cataccaatt gtattaaaaa caaagactaa
     7201 attttcactg gaacaagata ctcaaacagg gaaaaagctt atcataactg ctacccctat
     7261 atttgatgaa aatggaaact tagaatttac tgttgaaaat tgtagagata taactgaact
     7321 taataatata aaaaataaac ttgaagacac aaaaaagcaa gtaaaaaaat ataaatctga
     7381 agtagaaaca ctatacagaa ctgcacttag aattgaggat actgtaatta tggacggaat
     7441 agttatgaga ccaatcataa acacagtaaa tcatgtttct aaaacggatg taagtgtact
     7501 tttattgggt gaatctggaa caggaaaaag ctcacttgct agatatatac accacaacag
     7561 caatagatct aatggcccat ttataacaat aaattgtgca actatatctc cacaacttct
     7621 tgaatctgaa ctttttggat atacttcagg tgcttttaca ggtgcaagta ctaaaggtaa
     7681 agttgggctc gtagaattag ctaatggagg aactcttttt ttagatgaaa taggagatat
     7741 accacaaaat ctacaggcaa aatttttgca attaatacag gatagaactt ttactccagt
     7801 tgggagctta aaaaataaaa aggtcgatat aagaattata tctgctacaa atgttgattt
     7861 agtttcaaaa gtaaaagaaa aaaagtttag agaagattta tactatagat taaatgttat
     7921 agaaataaaa ttgcctcctc ttagagaaag aagggataat ctagtggaaa ttataaaata
     7981 ttattttaat aggtactcaa gtgacttcaa tctaaataaa actatttcta aagaagctat
     8041 ggatgctatt gcaaattata gattcccagg caatataaga gaattacaaa atataattca
     8101 aaaaatcttg cttacttgta cagataatca tataacaata gatgacttac caaacattct
     8161 tacaaaaaat atacatatta ccaataatgg aaataaaaca catatatccc aaattaataa
     8221 ggttattata tctgactcaa aatctactaa ttataaaaat aaaaattttg acactttaat
     8281 taaagagtat gaaaaaaata taattttgga tgcttatgaa aaatttggta gttcttataa
     8341 agtagccaaa caccttgaaa tctcacagtc taaagccaat aggttaatta gaaagtatac
     8401 taatacttaa ttttaaattt ga
//
LOCUS       DAEPIN010000112         8419 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.114, whole genome shotgun sequence.
ACCESSION   DAEPIN010000112 DAEPIN010000000
VERSION     DAEPIN010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8419
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.114"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            351..971
                     /locus_tag="KR250_003443"
     CDS             351..971
                     /locus_tag="KR250_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBH0069328.1"
                     /translation="MKVLLINGSPNQYGCTYTALNEITKVLSKHDIKTEILYLGKETI
                     PGCISCASCFETGKCIRNDKVNKLIEDLDNIDGIIIGSPVYFSSATGQLTSFLDRLFF
                     IAGSRMATKLGASVVSCRRGGASATFDQLNKYFSISNMPIVSSQYWNQVHGFTPEDVM
                     KDEEGLQTMRTLGENMAWLLKCISAGKKAGIKEPQYEETIMTNFIK"
     gene            complement(1296..2120)
                     /locus_tag="KR250_003444"
     CDS             complement(1296..2120)
                     /locus_tag="KR250_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraX"
                     /protein_id="HBH0069329.1"
                     /translation="MDFFKKGISGFTIKILALIFMTFDHIAAFMPQTMQIPIWFHWVG
                     RISAPLFIFMAVEGFYHTSNRKKYISRLYIWSVIMAIGNQIINNVFSHPEGAIIINNI
                     FSTLFLIAIYLQAIEFIKKFRKEKEIKYFIIGLLMIIIPIILGIFTVALLFKVTNRVI
                     ALFMILVPVPFLVEGGPIWIILGIIFYLCRGKKFSLSICYVLMCIFIFTTMSNGDYSL
                     KNSILQNYQWMMIASLPLMLLYNGEKGKSMKYLFYLYYPIHVYILYILGIYLINGF"
     gene            complement(2131..3534)
                     /locus_tag="KR250_003445"
     CDS             complement(2131..3534)
                     /locus_tag="KR250_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBH0069330.1"
                     /translation="MKWKITRNFIFTIVFVAISVVIINIISILYVISTNSFFKVVDSG
                     NNPEEFARSFEKDLYEKDGEFKLSKIGAEKLEKSNSWIQVLNDLGEEVYGVNVPKDTP
                     KKYTPFQMVNNYKYIETKYVNFVLEKYLNKKHLNIIVGIPSRDISRIILTYSQNNIKK
                     TLNKVIIITLVIDSVVALGVGYLFSRKLTKPISSVLWSIETMANGNYSLYLKDRGIYE
                     EVFKNINMLADTLRVNEVERKENEELREEWLANITHDIKTPLASIQGYAEIINDKDYE
                     FEEDEIQEYTEIIYNKSKYIKDLVDDLNLSTRLKNNTIVLDKKKINLVSLVRDIIIDI
                     LNDNRYKNRNIEFESNEDLIEVYVDSILFRRAITNLIFNSIVHNSEGTLISVEIVKKD
                     NIEIIIKDNGIGISKSDLKHIFKKYYRGTNTGEMHKGSGLGMAISKEIIEIHKGKIYV
                     SSEIGIGTKIIIEIKQN"
     gene            complement(3538..4263)
                     /locus_tag="KR250_003446"
     CDS             complement(3538..4263)
                     /locus_tag="KR250_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012450879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0069331.1"
                     /translation="MENRVLIIDDEVEILKLLETVLKKEGLNNIYTAKTKKEGLELFK
                     SINPDLIVLDIMLPDGEGYDICKEIRKTSNSPIIFLSAKTEELDKLLGLAIGGDDYVT
                     KPFSPKEVAFRVKAHLRRLSYFSDAQNESKNLNNNEEKIISFGPYILNESRAELIKDG
                     KSIGLFAKELKILSLFAHNQNQIISKEKLWDKVWGEDYVGFDNTIMVHIRKIREKLED
                     NPSKPEYILTIKGLGYKLAVKED"
     gene            complement(4375..5148)
                     /locus_tag="KR250_003447"
     CDS             complement(4375..5148)
                     /locus_tag="KR250_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0069332.1"
                     /translation="MMYLVKAEMIKILKSKSVWIIWLFLIFFGFFLIRKFDVLDTYAD
                     IFYKIEGSIPLIGLIMFIITSGNYIKEYDSNMTGLINTTKKGKKSVVLSKLVANGLIL
                     SIINISFILLVGIRGFSFFDFKNLNESIHNLWYFGNSNLNLTVIQMYIIVILTTIFAS
                     FIFAQIGLTLSSIFKSAVIPFILGGLIMAIPYFSVGFIPDKAIKFMSVTPNWIMMSQQ
                     MVKYNVPSILIVFSIVISIILMIVLTKITYENFTSSKRF"
     gene            complement(5154..6041)
                     /locus_tag="KR250_003448"
     CDS             complement(5154..6041)
                     /locus_tag="KR250_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0069333.1"
                     /translation="MNKLEIENLSKIYGKKVANDKITVTLENGVYGLLGPNGAGKSTL
                     MKQITTLIKPDEGQILYNGEDIFKMDADYRNILGYLPQEFGVYKNFTAKEFLQYIGAL
                     KGLRGKYLNSKIGELLDLVGLYDVRNKSIGKFSGGMKRRAGIAQVLLNDPKIIVLDEP
                     TAGLDPQERARFRNLIAKMSRDKIIILSTHIISDIESVAKETIMIKDGKLLMKGTHKD
                     ILSHMENKVYNVQVKNECEVDDIQSKYKVVSIQSDINSIVLRIVSEDIPSEDNIQPVQ
                     AKFEDVYMFYFDLEDAREV"
     gene            complement(6055..7077)
                     /locus_tag="KR250_003449"
     CDS             complement(6055..7077)
                     /locus_tag="KR250_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069334.1"
                     /translation="MVNWEIKKLAKSKSMFISLGILVLILMISIFITPVLETESYYID
                     KEKGRIEDTRSGIDIGNEKFQNKINVLKQFSNQTDTGEFSKKIELMSKKKLDNLSVNE
                     YKDISFWKVFNYRVTNSLINVGMLIIISIIISNIYVDEVVTSVKDIILSSKEKKKALN
                     SKIFVALLVPLVIYSVYLMGIFIITCMQQGVPVNGDLESFRIVDNVVALKTNLSITKY
                     VILNIGIELLMFEGWAMIAIFGSFISKSSISSISFFIFIISITKIMSVIHILPKKLLS
                     VVSNVNYYDLIFGFNKIIGNYLGDIMIFKVNIDIVNIAIGLLMAILIGAMSLCFLVIK
                     SDYISR"
     gene            7652..7828
                     /locus_tag="KR250_003450"
     CDS             7652..7828
                     /locus_tag="KR250_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069335.1"
                     /translation="MFIGEIDKCTHILTAYISSSYDYCNFLDTQLDDFISEYGETVVE
                     TCLHQVLLLVSRYN"
BASE COUNT         2997 a         1280 c          827 g         3315 t
ORIGIN      
        1 tcttatattt accactaatc agtgaaagtg tataactaaa tcctgtttca cttaaatctg
       61 tatttgatat acagtctata ctcatatatt acactctcct tttggttagt attgaacaaa
      121 aatgtgcgta cttgttttta gaataattta taataaaatt atagtgtaag aataattgaa
      181 agtcaatttt ataataggag gtgtatttac taaaaaaaaa gttattttaa ataggattct
      241 aagattagac atttctatat aaattgataa tattcatgaa aatcctattt tataaaaaat
      301 aatatctctt agaaaatcta ttaaataaaa ttcattggag gaaatttata atgaaagttt
      361 tattgataaa cggtagccca aaccaatatg gatgtactta tacagcacta aatgagatta
      421 ctaaagtatt gagtaaacac gatattaaaa cagaaatact gtatttaggt aaagaaacaa
      481 tcccaggatg tattagttgc gcatcctgtt ttgaaactgg aaaatgtatt cgaaatgata
      541 aagttaataa attgattgaa gatctggata atattgatgg tattattatt ggttctcctg
      601 tttacttttc tagtgcaact ggtcaattaa cttcttttct tgaccgtcta tttttcatag
      661 ctggaagtcg catggctaca aaattaggtg cttcagttgt gtcatgtaga cgtggtggtg
      721 caagtgcaac atttgaccaa ctaaataaat atttttcaat cagcaatatg cctattgtat
      781 catcacagta ttggaaccag gtacatggct ttactccaga ggatgttatg aaggatgaag
      841 aaggattaca gactatgcga actctaggag aaaacatggc atggcttctt aagtgtatat
      901 cagcaggtaa aaaagctgga attaaagaac cacaatatga agaaacaatt atgactaatt
      961 ttataaaata aataaaaaat gtactaatat taattaaaac aaaacactaa tttataaata
     1021 aaataagcat aaatgtagga taaaaagcac taaaacagag gtattgaatt ttatatcaaa
     1081 cctctgtttt attattacat taataaccaa accatgtata acatcatata tttctaaata
     1141 aattataata ttatattcta atgttattga aattacaata actgaatttc tgctcataaa
     1201 tttaaatatt ctacaaatct catctaaata acaattttaa tagacctaaa acaatcctca
     1261 tactgttcat tttgtattaa aaatatcact tacaatcaaa aaccatttat taagtatatt
     1321 cctaagatat acaatatata tacatggatt ggatagtata aataaaataa atacttcata
     1381 ctcttccctt tttctccatt gtaaagtaac attaaaggta gagaagcaat catcatccat
     1441 tggtaatttt gtaaaataga attttttaaa ctataatctc catttgacat tgttgtaaat
     1501 ataaatatac acattaaaac ataacaaata ctcaaactga actttttacc tctacacaaa
     1561 taaaaaataa tcccaagtat aatccaaatt ggtccacctt ctactaaaaa tggtactggt
     1621 accaaaatca taaataaagc aataactctg ttagtgactt taaaaagtaa agctactgta
     1681 aatattccta aaattatagg aatgattatc atcaataatc ctataataaa atatttaatt
     1741 tctttctctt ttctaaattt ctttatgaac tctattgctt gtaaataaat cgctattaaa
     1801 aataatgttg aaaaaatgtt atttataatt atagctcctt ctggatgtga aaatacatta
     1861 ttaattattt gatttcctat agccataata acagaccaaa tatacaatct acttatatat
     1921 ttttttctgt tactagtgtg ataaaatcct tcaacagcca tgaatataaa cagtggtgct
     1981 gatattctgc ctacccaatg aaaccaaatt ggtatttgca ttgtttgtgg cataaatgct
     2041 gctatatggt caaatgtcat aaatattaat gctaaaattt taatagtaaa cccacttata
     2101 ccttttttaa aaaaatccat atcatatctc ctaattttgt tttatctcta taataatttt
     2161 tgttcctatt ccaatttcac tacttacata aattttacct ttatgaatct ctattatttc
     2221 tttagaaata gccataccaa gacctgaacc tttatgcatc tcacctgtat tagtacctct
     2281 ataatatttt ttaaaaatat gtttcaaatc acttttgctt attccaattc cattatcttt
     2341 tattataatt tctatattat ctttttttac aatttctaca cttatcaaag ttccttcgct
     2401 attatgaact attgaattaa atataagatt ggttatagcc cttctgaata agattgaatc
     2461 tacatatact tcaattaaat cttcattact ttcaaattca atattcctat ttttatatct
     2521 attatcattt aaaatatcaa ttattatatc tctgactaaa ctgactaaat ttattttttt
     2581 cttgtccaga actatagtat tattttttaa tcgagtagat aaatttaaat catcaactaa
     2641 atcttttata tacttagact tattatatat aatttctgta tactcttgta tttcatcttc
     2701 ttcaaactca taatctttgt cattaattat ctctgcatac ccttgtatag atgctagagg
     2761 tgtctttata tcatgtgtta tatttgccag ccattcctct ctcaactctt cattttcttt
     2821 tctctcaact tcatttactc ttaaagtatc agctaacata tttatatttt taaaaacttc
     2881 ttcatatata cctctgtcct taagatataa actataattt ccattagcca tagtctcaat
     2941 actccatagt acgcttgaaa ttggttttgt aagttttcta ctaaataaat atcctacacc
     3001 taaagctacc acgctatcaa ttactagtgt tataatgata actttattta aagttttttt
     3061 aatgttattt tgagaatagg ttaaaattat tcttgatata tctctactag gtatacctac
     3121 tattatgttt aaatgttttt tgtttaaata tttttctagt acaaaattaa catactttgt
     3181 ttctatatac ttgtaattat tcaccatttg aaagggtgta tatttttttg gagtatcctt
     3241 tggcacattt actccataaa cttcctctcc taaatcattt aaaacctgta tccaggaatt
     3301 tgatttttct aatttttcag ctccaatctt agatagttta aattctccat ctttttcata
     3361 taaatctttt tcaaaagaac gtgcaaattc ttcaggatta tttccagaat ctacaacttt
     3421 aaaaaagcta ttagttgaaa tcacatacaa tatagatatg atatttataa ttactactga
     3481 tatagctaca aacacaattg taaatataaa atttctagta atcttccact tcatattcta
     3541 atcttccttt actgctaatt tatatccaag accttttatt gtcagaatat attcaggttt
     3601 tgaaggatta tcctctaatt tttccctaat ttttcttata tgaaccatta tagtattgtc
     3661 aaatccaaca taatcttctc cccacacttt atcccatagt ttttctttac ttataatttg
     3721 attttgatta tgtgcaaata aagataggat ttttagttcc tttgcaaaca gtcctataga
     3781 ttttccatct tttataagtt ctgccctact ttcatttaat atatatgggc caaatgatat
     3841 aattttttct tcattattat ttagattttt actttcgttt tgagcatcac taaaataact
     3901 taatcttctc aaatgcgctt taactctaaa agccacttcc ttagggctaa aaggttttgt
     3961 aacataatca tctcctccaa tcgctagtcc aagtaactta tctaattctt cggttttagc
     4021 tgataaaaat attattggag aattggaggt ttttcttatt tctttacaaa tatcataacc
     4081 ttcgccatct ggtaacataa tatccaatac aatcaaatca ggattaatac ttttaaataa
     4141 ttctaatcct tcttttttag tttttgcagt atatatatta ttaaggcctt ctttttttag
     4201 gacagtttct aaaagcttca atatttctac ttcatcatct attattaaaa cacgattttc
     4261 cataaacaac ctcctgtaat aatttacata tatattagca tatattttat ctaaaattca
     4321 tttatcttat ttttaattaa actaaaatat aaataaaatt actattattt ttagttaaaa
     4381 ccttttgctt gatgtaaaat tttcatatgt tattttagtt aacactatca ttaatataat
     4441 tgagattact atagaaaaaa ctatcaaaat acttggtaca ttatacttaa ccatttgttg
     4501 gctcatcata atccaatttg gagtcactga cataaatttt atagctttat ctggtataaa
     4561 acctacacta aagtatggaa ttgccattat caatccacct agtataaatg gaattacagc
     4621 cgatttaaat atagatgaaa gtgttaagcc tatttgtgca aatataaaag aagcaaaaat
     4681 agtagtcaat ataacaatta tatacatttg tattactgta agatttaaat tactatttcc
     4741 aaagtaccat aagttatgaa tcgattcatt taaattttta aaatcaaaaa aactaaaacc
     4801 tctaattcct actaacaata taaaagaaat attaataata gataaaatta atccattagc
     4861 tactagtttt gataatacta ctgatttttt tcccttttta gttgtattta ttaaacctgt
     4921 catattagaa tcatattctt taatgtaatt tccagaagtt attataaaca ttatcaaacc
     4981 tattagaggt attgagcctt ctatcttgta aaatatatct gcataagtat ctaaaacatc
     5041 aaactttcta atcagaaaaa atccaaaaaa tattaaaaac agccaaatta tccaaacact
     5101 ctttgatttt aatattttta tcatttcagc tttaactaaa tacatcattt ctactacacc
     5161 tcccttgcat cttctaaatc aaaataaaac atgtatacat cttcgaattt tgcttgaact
     5221 ggttgtatat tatcctcgct tggaatatcc tctgaaacaa ttctaagtac tattgaatta
     5281 atatcacttt gaatacttac aaccttatat ttagattgta tatcatcaac ttcacattca
     5341 tttttaactt gaacattata tactttattt tccatgtgac ttaatatgtc tttgtgtgtt
     5401 cctttcatta ggagtttgcc atctttaatc attattgttt cctttgcaac tgattctata
     5461 tcggaaatta tatgagtaga aagtattatt atcttatctc tagacatttt tgctattaaa
     5521 ttcctaaatc tagctctttc ttgtgggtca agacctgctg ttggctcatc aaggactatt
     5581 atcttagggt cgtttagtaa aacttgtgct atcccagctc tcctcttcat acctcctgaa
     5641 aattttccta tagacttatt tctcacatca taaagaccta ctaaatctaa taattctcct
     5701 atcttactat ttaaatattt tccccttaaa ccttttaaag ctccaatata ctgtaaaaat
     5761 tcttttgctg taaaattttt ataaactcca aattcttgtg gcaaatatcc taaaatattt
     5821 ctataatcag catccatttt aaatatatcc tcaccattat aaagtatctg accttcatca
     5881 ggttttatca acgttgttat ttgtttcatt aatgtagatt ttcctgctcc atttggtcct
     5941 agaagaccat aaactccatt ttctagagtt actgtaatct tgtcgtttgc tactttttta
     6001 ccatatatct ttgaaagatt ttctatttct aatttattca tcttaactcc ccttttatct
     6061 acttatataa tctgatttta ttactaaaaa acataatgac atagcaccta tcaatattgc
     6121 catcaaaagt cctattgcta tattgacaat gtctatattc actttaaata tcataatatc
     6181 acctagataa tttcctatta ttttattaaa accaaatatc aaatcataat aatttacatt
     6241 tgatacaact gaaagtaatt ttttaggtaa tatatggatt accgacatta tcttagttat
     6301 agatataata aatataaaaa aacttattga agatattgaa gacttactta taaaagaccc
     6361 aaatatggct atcatggccc atccttcaaa catcaaaagc tctatgccta tatttaatat
     6421 tacatacttt gttatactta aattagtttt caatgcaact acattgtcta ctattctaaa
     6481 agattctaag tctccattaa caggtactcc ttgttgcata caagttataa taaatattcc
     6541 cattaaataa acagaataaa ttactaatgg aactaataaa gcaacaaata ttttagaatt
     6601 taatgctttt ttcttctctt ttgatgatag tatgatatct tttaccgaag taacgacttc
     6661 atctacgtaa atattagata taattataga tataatgatt aacattccta catttataag
     6721 tgaatttgta actctatagt taaatacttt ccaaaaacta atatccttat attcattcac
     6781 acttagatta tcaagttttt tctttgacat taattcaatt tttttagaaa attcacctgt
     6841 atctgtttga tttgaaaact gctttaaaac attaatctta ttttgaaatt tctcattacc
     6901 tatgtcgata ccacttctcg tatcttctat acgtcctttt tctttatcta tataataact
     6961 ttctgtttct aaaacaggcg taataaagat actaatcatt agtattaaca ctaatatgcc
     7021 tagagatata aacatactct tagatttagc caattttttt atttcccaat ttaccaatat
     7081 atttctcctc ctaaatcatt ttaattacac atatatatta aaacttaaat cttatataaa
     7141 tataatacta accttaaata aagcttaaat tattttataa tttataggtg tgtttattat
     7201 tggagttaca tttattattt ttctgggaaa ttatacattt tcaattattt ttattatttt
     7261 tgatattttt attgattgga aattttattg ttgttcaaat tgtagttatt ctttaaattt
     7321 aaagatggag ttttttaaga atagaaccta ctgtccttat tatggaaaga ttattaaata
     7381 gttgcatata actttattta tcttgaatct tatttttaaa aacaattata attttagaat
     7441 attttctatt ctcaaacaag catgttgaat aatgaagcat cttctagtat tatatacttg
     7501 tagaataaaa ctaaattggt aaaactggga atatttaacc ttaaggagtt aagttataga
     7561 aactagtact aaaaaatttt ataaattatg ttaattgact aatagaatgc gactttttat
     7621 ttttactaaa ttttttaggg ggatggataa tatgtttatt ggagaaatag acaaatgtac
     7681 acatatacta actgcttaca tcagtagttc atatgattat tgtaattttt tagatacaca
     7741 gttagatgat tttatatcag aatatggaga aacggtagtt gaaacctgct tacatcaagt
     7801 cttattatta gtaagtagat acaattaaaa ctaaattttt ctagaaaaac ctttttagat
     7861 ttatctactc taataaatat ataatttttt gtactttata agtaactata acttctgcta
     7921 aagtaaagat gtattgggag tacatcttta ctttcccatt atagaagtta aaagcataca
     7981 ccataaaatt aatcatccaa tacaaattat atatttccta tgtctaactt aatttacaag
     8041 aatcattcta tatgcatatt ctgcaatcca ctaaacatgg ctctaaacct ttactatttt
     8101 atacattctc tataaatcat acaaatcata tataaaatcc ccctaatatg tttaaaccac
     8161 ctataaaata acaccaatat ttagaatatt caatatcaac tgcataactg gtcaataaaa
     8221 acgtataact agacaaattt caatatttat ataaataata caagaaaaac aagcatttta
     8281 tttatataag ttttttattt tttgtttata ttaaaaaact atattgatta atttatttta
     8341 tttagtaatt ataaaaattc gtcttagaat atattctata tttgtagatt agctttaata
     8401 tttttaattt cacaaaaat
//
LOCUS       DAEPIN010000113         8368 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.115, whole genome shotgun sequence.
ACCESSION   DAEPIN010000113 DAEPIN010000000
VERSION     DAEPIN010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8368
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.115"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            95..250
                     /locus_tag="KR250_003451"
     CDS             95..250
                     /locus_tag="KR250_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069336.1"
                     /translation="MKGILKELTAKLYSFFYFMFWGFYFSAGYFFYKNICTSFLVRPY
                     FSDFEHK"
     gene            380..1393
                     /gene="aroF"
                     /locus_tag="KR250_003452"
     CDS             380..1393
                     /gene="aroF"
                     /locus_tag="KR250_003452"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HBH0069337.1"
                     /translation="MIVVLKMGADKNEVKKLIEAIGREGVEVNPIDGTELTVLGLVGD
                     TSKIDAKRIEANKVVEKVMHVVEPFKKANRKFHPEPSIINVNGMEIGSKKIAMIAGPC
                     SVETEDQIVSIAKDVKKSGAGFLRGGAFKPRTSPYAFQGLKYDGLDLLKKAKEKTGLP
                     IVTEIMSTQDIDIFEENVDVIQVGARNMQNFDLLKELGKTNKTILLKRGLSATIEEWL
                     MSAEYIMAGGNENVVLCERGIRTFETYTRNTLDLSAILAVKKLSHLPVIVDPSHAAGK
                     SWMVDSLSKAAIAVGADGLIIEVHNDPAHALCDGKQSIKPNEYDELISELKTIASAVG
                     REI"
     gene            1415..2479
                     /locus_tag="KR250_003453"
     CDS             1415..2479
                     /locus_tag="KR250_003453"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HBH0069338.1"
                     /translation="MEEKLLVNLKENSYNIFIKKNILKNIGEEIKKIYFGNKIFIVTD
                     ENIAKLYLDKVKNNLSESGFEIKSLILKSGEETKSFNTLPKIYDSLLDFKLTRKDLII
                     TLGGGVIGDLGGFAAATFLRGISFIQIPTSLLAQVDSSVGGKVGVDLERGKNLVGSFY
                     QPKAVFIDPNVLLSLPEKFYRDGMAEVIKYGCIKDKTFFNKLLSLKNREEVMQHIEYI
                     IHKCCFIKKTVVEADEKDFGDRMLLNFGHTLGHAIEKYYNFTGYTHGEAVAIGMYKIT
                     LESEKIGITKKGTSELIKSILMNYNLPYEIELRDNKSIVDAISLDKKNLGDTLKIILL
                     KDIGESIIYNTTSKFFQEVR"
     gene            2479..3792
                     /gene="aroA"
                     /locus_tag="KR250_003454"
     CDS             2479..3792
                     /gene="aroA"
                     /locus_tag="KR250_003454"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HBH0069339.1"
                     /translation="MGSLKIYPSKLSGDVKIPPSKSMAHRAVICSSLSNGKSRISNID
                     FSDDIIATIRAMTSLGAIIEKKEDILEISGIFSKEGILNRENQLNQPKLTIDCNESGS
                     TLRFLVPISLAFDGVKRFIGRGNLGKRPLDTYYEIFDRQNIKYSYKENQLDLIISGKL
                     KPDEFRVKGNISSQFITGLLFILPTLESDSKIIITTELESKGYLDLTLSTIKDFGVEI
                     INNNYKEFIIKGNQTYKARDYKVEGDYSQGAFYLSADAIGEDISILDLKEDSLQGDSE
                     VVEILSRMGMEILREGNKIKGITNGLNGTLIDASQCPDIIPVLSVVASLSIGRTTIIN
                     AGRLRIKECDRLHAINVELNKLGANIEEKEDSLIIEGVSKLNGGVEVWSHKDHRIAMT
                     LAIASCRCDKPIILKDFECVSKSYPHFFKDFKMLGGRIDEWNMGK"
     gene            3770..4849
                     /gene="aroC"
                     /locus_tag="KR250_003455"
     CDS             3770..4849
                     /gene="aroC"
                     /locus_tag="KR250_003455"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HBH0069340.1"
                     /translation="MSGIWGNNLKVSIFGESHGNAIGINIDGLPSGIELDLDNIDKEM
                     KRRAPGKNSISTSRNESDIPEILSGYFNGRTTGTPLCAIIRNSDTRSKDYGELKNLMR
                     PGHADFTGNVRYSGFNDYRGGGHFSGRITAPLVFCGAICKQILSQKGIEIGAHIKKIK
                     NIEDMSFDYVNISKQQLSNLQTLELPLLDLSKEEAMKNTIIDAKNQGDSVGGIIECAV
                     VGINVGLGNPFFDSVESTLSHLLFSVPAVKGVEFGLGFELADMYGSQSNDEMYYEGNQ
                     VKSKTNNNGGIIGGITTGMPIIFKVAIKPTPSISRQQNTVNIKDKKDDILYIKGRHDP
                     CIVQRAIPVIEAVTAIGIFDLMKGR"
     gene            4852..6048
                     /gene="pheA"
                     /locus_tag="KR250_003456"
     CDS             4852..6048
                     /gene="pheA"
                     /locus_tag="KR250_003456"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HBH0069341.1"
                     /translation="MEDLTRYRNEIDNIDKELIQLFEKRMNTVLEIARHKMKNNTTIL
                     QKDREEKVLSRAVDNLDNKAYSQETVQFFNSIMEISRGLQKRFIDNDGEQKIEFSLDS
                     SNKNFDLSNVNKYKSLKDELNKKNILVGFPGKSGSFTEEALNKFFNKKISKKQFKEFE
                     DVFIALKNKEINYGIIPIENSSTGAISETYDLLRKYGFYIVGEECIKIDQNLIGIKDT
                     KLEDIKEIYSHPQGIGQCSEFLKQNSTWKLIPFNNTATSAELVRKLQDKTKAAIASKK
                     AASIYGLEIISPCINDIANNYTRFVVISNQIHIEEESNKMSVVFSVEHEAGKLYKVLG
                     YFAKNNINMTKIESRPMKNASWRYFFYIDFECSIYNAKVYDLLELIEHNTAYFKFMGV
                     YKNKTE"
     gene            6095..6904
                     /gene="aroE"
                     /locus_tag="KR250_003457"
     CDS             6095..6904
                     /gene="aroE"
                     /locus_tag="KR250_003457"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HBH0069342.1"
                     /translation="MNFFGLVGEKLSHSVSPQIHKRVFEILNIESAYKNFEISKEDIS
                     KLDGAIKLLGIQGVNVTVPYKERIMKYLDFISPEAKRIGAVNTILLRENMLYGYNTDY
                     FGLDSMFKMANIDVQGKVAVILGTGGASKAALTYFIDSGIEKLYVSTRKKDDKKLLNS
                     KAILIDYEELKHIKGDIILNATPVGMYPNVGISPVSKSIIQNFDILIDLIYNPGETEF
                     LRIGNSMGKKTCDGLYMLVGQAIKSQEIWQDTKIDNSILDVIYNELKLEFL"
     gene            6916..7449
                     /locus_tag="KR250_003458"
     CDS             6916..7449
                     /locus_tag="KR250_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HBH0069343.1"
                     /translation="MINKTKEKLILIGMPGSGKTTIGKLLAKEYNCSFCDMDDYIIQI
                     SQKSIAELFSEGEDIFRNYETQACRELSISDKTVISTGGGVIKKDINMEILKETGIII
                     FIDRPIQKILEDININSRPLLKNGKDRLYNLYNERINLYKKFSDIEILNDKSLNNAVY
                     NITNAVSENFKFDFKEK"
     gene            7470..8309
                     /locus_tag="KR250_003459"
     CDS             7470..8309
                     /locus_tag="KR250_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HBH0069344.1"
                     /translation="MNIVIVGLGVIGGSFAKALKKAGYENVFGVDVDLETLKKAEKAK
                     IIKKGCTTGKELFKKADLIILSIYPRLVVDFLNNNKNFFKKGTIITDTTGIKETLIND
                     VLQIIPDDIDFIFGHPMAGREKKGIDFASEQVFNGANYIITPTGRNNIKNLELVENLI
                     LEIGFKRVKKLTSQKHDEIIAFTSQLPHVMAVALINSDEEGRDTGKFIGDSYRDLTRI
                     ANMNEDLWSELFLGNRDNLLKVIENFESEVNLVKEAIFNNDKNKLIEYFKKSSIRREA
                     LEK"
BASE COUNT         3434 a          836 c         1509 g         2589 t
ORIGIN      
        1 gaaaatattt atagaaaata ttgacaatta tattttcttt gttataatta ttaaacaaga
       61 agaataataa attataaaaa tggaggataa tactatgaaa ggaatattaa aagaactaac
      121 agcaaaactt tatagctttt tttactttat gttctggggc ttttatttta gcgctggcta
      181 tttcttttac aaaaatatat gtacttcctt tctggtgagg ccttatttta gtgattttga
      241 gcataagtaa attttataaa aatgattatt ttatatattt atataagatt tagtgcttga
      301 tagattattt tagagaactc attagtgagt tctctttttt attttaaata taaaattata
      361 atattgggag gtagtaaaaa tgatagtagt attaaaaatg ggagcagata aaaatgaagt
      421 taaaaaatta attgaggcta ttgggagaga aggagtagaa gttaatccca tagatggaac
      481 agaattaact gttttaggtt tagttggaga tactagtaaa atagatgcca aaagaataga
      541 agcaaataag gtagttgaaa aagttatgca tgtggtagag ccttttaaaa aggccaatag
      601 aaaatttcat ccagaacctt caatcataaa tgttaatgga atggaaattg gttcaaagaa
      661 aattgctatg atagctgggc cttgttctgt tgaaacagaa gaccaaatag tatctatagc
      721 taaagatgtg aaaaaatcag gtgcaggatt tttgagaggt ggagcattca aaccaagaac
      781 atcaccatac gctttccaag gattgaaata tgatggattg gatttattaa aaaaagcaaa
      841 agaaaaaaca gggcttccta tagttacaga aataatgtca acacaagata tagatatatt
      901 tgaagaaaat gtagatgtaa ttcaagttgg agctagaaac atgcaaaact ttgatttatt
      961 aaaagaatta ggtaaaacaa ataaaacaat acttttaaaa agaggattat ctgctacaat
     1021 tgaagagtgg ctaatgtcag cagagtatat aatggctgga ggaaatgaaa atgtagttct
     1081 ttgtgaaaga ggtataagaa cttttgaaac atatactaga aatactttgg atttaagtgc
     1141 aattttagct gtgaagaaac ttagtcattt accagttata gttgacccaa gtcatgcagc
     1201 aggtaaatca tggatggttg actctttatc taaggcagca attgcagttg gagcagatgg
     1261 tctaataatc gaagttcata atgacccagc acatgcactt tgtgatggaa aacaatcgat
     1321 taagccaaat gaatatgatg aattgataag tgaattaaag actatcgcat cagcagttgg
     1381 tagagaaata taatctataa aaggatggtt aaacgtggaa gaaaaactat tagtaaattt
     1441 aaaagaaaat agctacaata tatttattaa aaaaaatata ctaaagaata taggagaaga
     1501 aataaaaaaa atatattttg gaaataagat ttttattgta actgatgaaa atatagcaaa
     1561 attatacttg gataaagtaa aaaataattt aagtgaatct ggatttgaaa ttaaatcttt
     1621 aatattaaaa tctggtgaag aaacaaaatc atttaatact cttcctaaaa tatacgatag
     1681 tttacttgac tttaagttaa ctagaaaaga tttgattatt actttaggag gaggggtaat
     1741 aggtgattta ggtggatttg cagcagcaac ttttttaaga ggtataagtt ttattcaaat
     1801 tccaacatct ttattagcac aagttgatag ttctgtaggt ggaaaagttg gagttgacct
     1861 tgagagaggc aaaaacttag tgggaagctt ttaccaacca aaggctgtgt ttatcgaccc
     1921 aaatgtatta ttatcacttc cagaaaaatt ctatagagat ggaatggcag aagttataaa
     1981 atatggatgt ataaaagaca aaactttttt caataaactt ttaagtttaa aaaatagaga
     2041 agaagtaatg caacatatag aatacattat acataaatgt tgctttatta aaaaaactgt
     2101 agttgaagct gatgaaaaag actttggaga tagaatgctt ctaaactttg gccatacatt
     2161 aggtcatgct attgagaaat actacaattt tacgggttat acccatggag aagctgttgc
     2221 cataggaatg tataagataa ctttagaaag tgaaaagata ggaattacaa aaaaagggac
     2281 atcagaactt ataaaatcaa ttttaatgaa ttataatttg ccttatgaga ttgaattaag
     2341 agataataaa tctatagtag atgctatatc tcttgataaa aaaaatctag gtgatacatt
     2401 aaaaataata cttttaaaag atattggaga gagtattata tataatacta cttctaaatt
     2461 ttttcaggag gtaagataat gggaagtcta aaaatatatc caagtaaatt atctggagat
     2521 gtaaaaatac caccatcaaa aagtatggca catagggcag ttatttgttc atctctaagc
     2581 aatggaaaaa gtagaatttc taatatagat ttttctgatg atataatagc tactataaga
     2641 gcaatgacat cattaggagc tattatagaa aagaaagaag atattttaga aatttcggga
     2701 attttttcta aagaaggtat tttaaataga gagaatcagt tgaatcaacc taaattgaca
     2761 atagattgta atgaatcagg ctctacattg agatttttag tacctatatc tttagcattt
     2821 gatggtgtaa aaaggtttat aggtagagga aatcttggaa aaagaccact tgatacttat
     2881 tatgagatat ttgacagaca gaatataaaa tatagttaca aagaaaatca gttagatttg
     2941 attatatcag gtaagcttaa accagatgaa tttagagtaa aaggaaatat aagttctcag
     3001 tttatcacag gtttactgtt tatattgcct acgctagaga gtgattcaaa gataataatt
     3061 actactgagc tagaatctaa aggatattta gatttaactc tttcaacgat aaaagatttt
     3121 ggggtagaaa ttataaacaa taactataaa gaatttatta taaaaggaaa tcaaacgtat
     3181 aaagcgagag attataaagt agaaggagat tattctcaag gagcatttta tttatcagca
     3241 gatgctatag gagaagatat tagcatatta gaccttaaag aagattctct tcaaggggat
     3301 agtgaagtag ttgaaatttt atctaggatg ggaatggaaa tattaagaga aggcaacaaa
     3361 ataaaaggaa taacaaatgg tttaaatggt actttgatag atgcatctca atgcccagat
     3421 ataatacctg tattatctgt agtagcatcc ttaagtatag ggaggactac aataattaat
     3481 gctggtaggc ttagaattaa agaatgtgat agacttcatg caataaatgt tgagttaaat
     3541 aaacttggag caaatataga ggagaaagag gatagtttga taatcgaagg agtaagcaag
     3601 ttaaatggtg gagtagaggt gtggagtcac aaagaccata gaattgctat gacacttgca
     3661 attgcttctt gtagatgtga taaaccaata atattaaaag attttgagtg tgtatcaaaa
     3721 tcttatccac atttttttaa agattttaag atgttaggag gtcgaattga tgagtggaat
     3781 atggggaaat aatttaaaag tatcaatttt tggagaatct catggaaatg ctataggtat
     3841 aaatatagat ggtcttcctt cggggattga acttgacctt gataatattg ataaagaaat
     3901 gaagagaaga gcacctggaa aaaattctat atctacttca agaaatgaaa gtgatatacc
     3961 agaaattcta agtggatatt ttaatggaag aactacagga acgccattat gtgcaatcat
     4021 aagaaatagt gatactcgtt caaaagatta tggagagctt aaaaacttaa tgagaccagg
     4081 acatgcagat ttcacaggta atgtgagata ttctggattt aatgactata gaggtggagg
     4141 tcatttttca ggtaggataa cagctccatt agtattttgt ggggctatat gtaaacaaat
     4201 tttatcacaa aaaggaatag agataggagc acacataaaa aaaattaaaa atatagaaga
     4261 tatgagtttt gattatgtaa atatttcaaa acaacagtta tcaaatttgc aaactttgga
     4321 actaccttta cttgatttat caaaggaaga agccatgaaa aatacgatta tagatgcaaa
     4381 aaatcaagga gattcagttg gtggtataat tgagtgtgca gtagtaggta ttaatgtagg
     4441 gcttggaaat ccattttttg actctgttga atctacttta tcacatttat tgttttctgt
     4501 tcctgctgta aaaggtgtag agtttggttt aggttttgaa cttgcagata tgtatggctc
     4561 tcaaagtaat gatgaaatgt attatgaagg aaatcaagtt aagagcaaaa caaataacaa
     4621 tggaggaatt ataggaggga ttactactgg aatgccaata attttcaaag tggcaattaa
     4681 gccaactcca tcaattagca gacaacaaaa cacagttaat ataaaagata aaaaggacga
     4741 tattttatat attaaaggca gacatgaccc ttgtatagtt caaagggcta tacctgtaat
     4801 agaagcagta acagccattg gaatttttga tttgatgaaa ggacgataat aatggaggat
     4861 ttaacgagat atagaaatga gattgacaat atagataaag agcttattca attatttgaa
     4921 aagagaatga atacagttct tgaaattgcc agacataaaa tgaaaaataa cactactata
     4981 cttcaaaaag atagagaaga aaaagtacta agtagagccg tagataattt agataataaa
     5041 gcatattcac aagaaacggt acagtttttt aattctataa tggaaattag tagaggatta
     5101 caaaaaagat ttatagataa tgatggtgag cagaaaatcg agtttagttt agattcatca
     5161 aataagaatt ttgatttgtc aaatgtaaat aaatataagt ctttaaaaga tgaattaaat
     5221 aaaaaaaata tcctagtagg ctttcctgga aaatcaggtt catttacaga agaagcacta
     5281 aataaattct tcaacaaaaa aatatcaaaa aaacaattta aagaatttga agatgttttc
     5341 atagcactta aaaataaaga aataaactat ggaataatcc ccatagaaaa ttcatctaca
     5401 ggagctatct ctgaaactta tgaccttttg agaaagtacg gtttttatat agttggagaa
     5461 gaatgtataa aaatagacca aaatttaata gggataaaag acacaaagct tgaagatata
     5521 aaagaaatat attctcatcc acaaggtata ggacaatgta gtgaattttt aaaacaaaat
     5581 agtacatgga aattgatacc ctttaataac acagcaacaa gtgcagaact tgtaagaaaa
     5641 cttcaagata aaacaaaagc agcaattgca agcaaaaaag ctgcaagtat ttatggatta
     5701 gaaataataa gtccttgtat aaatgatata gcaaacaact atacaagatt tgtagtaatc
     5761 agtaatcaaa tacacattga agaagaatca aacaaaatga gtgtagtatt ttctgttgaa
     5821 catgaagcag gaaaacttta taaagtactt ggatactttg ctaaaaataa tataaatatg
     5881 acaaaaatag aatcaagacc tatgaaaaat gcctcatgga gatatttctt ttatatagat
     5941 tttgagtgtt ctatatacaa tgctaaagtg tacgatttat tagaattgat agaacataat
     6001 acagcatatt ttaaatttat gggagtatat aaaaataaaa ctgaatagat aagataaatt
     6061 tttatttaat ttataattat taggaggtgt aagtatgaat tttttcggtc tagtaggtga
     6121 aaaactctct cacagtgttt cacctcaaat ccacaagaga gtattcgaga tattgaatat
     6181 tgaaagtgct tataaaaact ttgaaatttc aaaagaagat atatctaagc tagacggtgc
     6241 tataaaatta ttaggaatac aaggggtaaa tgttactgta ccatataagg agcgtattat
     6301 gaaatatctt gattttatat caccagaggc aaaacgtata ggagcagtta atacaatttt
     6361 actcagagaa aatatgttat atggttacaa tacagattac tttggtcttg atagtatgtt
     6421 taaaatggct aatattgatg ttcaaggaaa agtagcagtt atattaggaa ctggaggtgc
     6481 ttctaaagct gctcttacat attttattga ttctggtata gaaaagcttt atgtttcaac
     6541 tagaaaaaag gatgataaaa aattattaaa tagtaaagct atattaatag attatgaaga
     6601 acttaaacat ataaaaggag atataatatt aaatgctaca ccagtaggta tgtaccctaa
     6661 tgtaggaatt tcaccagtgt caaaatctat tatacaaaat tttgatatat taatagattt
     6721 aatttacaat cctggagaaa cagagttttt aagaattggt aatagtatgg gtaaaaaaac
     6781 ttgtgatgga ttatatatgc tggtggggca agcaataaaa tctcaggaaa tttggcaaga
     6841 tactaaaata gataatagta ttttagatgt tatatataat gaactaaaat tagaattttt
     6901 gtaagggaga ttgttatgat aaataaaaca aaagaaaaat tgatattaat aggtatgcca
     6961 ggtagtggaa aaactacaat tggtaaactt ttggccaaag aatataattg tagtttctgt
     7021 gatatggatg attatataat tcaaatatct caaaaaagta tagcagagtt gttttcagaa
     7081 ggagaagata tctttaggaa ctatgagact caagcatgta gagaactttc tataagtgat
     7141 aaaacagtga tatctacagg tggaggtgta ataaaaaagg atattaatat ggaaatacta
     7201 aaagaaacag gtataataat ttttattgat agacctatac aaaagatttt ggaagatata
     7261 aatattaatt caagaccatt attaaaaaac ggaaaagata gattgtataa tctttacaat
     7321 gagagaataa atctttataa aaaattttca gatatagaga ttttaaatga taaatcatta
     7381 aataatgctg tatataatat tacaaatgca gtcagtgaaa attttaaatt tgactttaaa
     7441 gaaaaataat ctagggggaa ataaaaataa tgaatattgt tattgtagga ctaggagtta
     7501 taggaggctc ttttgctaaa gcattaaaaa aagctggata tgaaaatgta tttggagtag
     7561 atgtagattt agaaacttta aaaaaagcag aaaaagcaaa aatcataaaa aaaggatgta
     7621 ctacaggtaa agagttattt aaaaaagctg atttaattat attatctata tatcctagat
     7681 tagttgtaga ttttctaaat aataataaaa acttttttaa aaaagggact ataattactg
     7741 atactacagg aataaaagaa acacttatca atgatgtgtt gcaaattatt ccagatgata
     7801 tagattttat atttggacat ccaatggcag gtagggaaaa aaagggtatt gattttgcaa
     7861 gtgaacaagt ttttaatggc gcaaattata taattactcc aactggcaga aacaacataa
     7921 aaaatttaga gttagttgaa aatttaattt tagagattgg atttaaaaga gtaaaaaagt
     7981 taacttcaca aaaacatgat gagattatag cttttacttc tcagttacct catgttatgg
     8041 cagttgctct tataaatagt gatgaagaag gtagagatac gggtaaattt attggagata
     8101 gttatagaga tttaactaga attgcaaata tgaatgaaga cttatggagt gaactttttt
     8161 taggaaatag agataattta ctaaaagtta tagaaaactt tgaatcagaa gtaaatttag
     8221 taaaagaagc tatatttaac aatgataaaa ataaattaat agagtatttt aaaaaatcat
     8281 caataagaag agaagcttta gaaaaataaa tattaatatt taagctatat atagatgggt
     8341 ataaatataa gcgatagtgt atttaatt
//
LOCUS       DAEPIN010000114         8347 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.116, whole genome shotgun sequence.
ACCESSION   DAEPIN010000114 DAEPIN010000000
VERSION     DAEPIN010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8347
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.116"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            548..2782
                     /locus_tag="KR250_003460"
     CDS             548..2782
                     /locus_tag="KR250_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBH0069345.1"
                     /translation="MQNHNYIKMLNKKVEVLSKELDSRNFKLKTLNEIDFFSKIPNRE
                     FMYQHIDDCISKNIHIGIMLLNIDNFKLFNDIYGYLVGENLLITLTQNLLHFIPESKG
                     IVAYLKGEGFLIVLPNVSNKELNNMGATIIESARELKIKLPNEKEIYSNVTFSIGSTI
                     WNGEPNMTTLMLFNQVYSALKKAKSEGKNRYILFDFKDNSFYNKLENDKEFLSEISHV
                     LQTKQIEVFYQPLYNIKNNTIVGCEALARWRHPIKGLLTPDKFIPLFEQFGLITELDL
                     HLFEENCKNIRRWIDNNDVFVPICCNFSRLHFFNPNFPSMLKGITEKYSVSTSNFSIE
                     ITENILIENTNTIIQQLAELRSYGFSVSMDDFGSGYSSFGMLQNLPFDIIKIDRIFLS
                     HNLNDFKNTTILYSIINIARALGMLTVCEGVETEQHVEFIKSINCDIAQGYYYAKPME
                     CKEFEKLLSKENNSEKMFIKYSDIGKSLIEKIFDGFFIMQDMKKLRANVTEDIQWFDF
                     YNKTEITSFPKVIEHFEKHIKGKKFSILYKSITPHNTESDSLLISGEAVLSDEASEHQ
                     KYSNFYFSANCIISENKLLLAKLHLDLIKTAGYINEFESYSIPKMTNTHLDESNILSQ
                     FYSMLPVGIIRYDLFGDMIITYMNEEMFDIIGYTKEQFFGEMGGNLRAIVHPDDLDFV
                     YKKSLDMIDTNKIEPFLYRFIKRDGQIVTVMYTQCNLSAIDGRSIAQGMYVDVNKIKN
                     LTKI"
     gene            2813..3646
                     /locus_tag="KR250_003461"
     CDS             2813..3646
                     /locus_tag="KR250_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBH0069346.1"
                     /translation="MFKEIFLRTFPGFLIIRDSNYRIIFINDNLKNLIKSYMQDNPLG
                     MTNIEIAKKLPDNIAKFFTDSHNIQLDWEKNYPYDKISNWILEFKKDTTSYWNVLEYK
                     VDVDEKTYIITMANDITKLYEENKRNLHYSITDPLTGAYNRKYLNDRFDIFIGDYIVL
                     IDLDNFKMINDYEGHNVGDKILCDFVSLLNKELINSTSIIRLGGDEFIVIFSSDVDKN
                     YVYSQLEALRENFLKVFSKYKYLSFSYGVDTVKRNLKLTIVELDKKMYKNKKKFDKND
                     Y"
     gene            3886..5619
                     /locus_tag="KR250_003462"
     CDS             3886..5619
                     /locus_tag="KR250_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBH0069347.1"
                     /translation="MSILLKKAPKLAKHIITSFYINRDIDEVLKYLCENVTWIGPGEQ
                     EFLTSFNEIKNYFYAGQDEIPSCDINNDIFETVSEDENRCMVLGRYTVRTKENTQMIL
                     EVNQRCTFEIIEDRGKLLVKHMHISNPYGEMQLDEYFPTKIGTQSYDYLQRLLKEKTE
                     VIEMITNNINGGLKGSNDDNTYSFFYVNEGLPKILGYTYDEFMEMSGGSAVGAVYPPD
                     LPKALEDCERCFAKGPTYSSEYRIRKKDGTLMWVLDSGMKSLNSDGIVKINSIITDIT
                     QLKNIESELKLERERYRIALQNITDIMFEYDIENDTLIKYQRVEIDKKIELENFETKN
                     YSKLLESGKIIHLDDIGKLLEVLHGNLRETIEIRQVNSLTKNEWRWIRVQCSVIYDSD
                     HNPIKTIGVLKDITEDKSKLESLINQAQRDPLTQLYNQRVSQNLIEEYLCSSDSKNND
                     ALLVIDIDDFKTVNDTFGHLEGNEVLVAVSKILLHNTHDKDIVARIGGDEFTIFIKSL
                     TKDLIIKITNDILNDASKIKVKDNHKITLSIGIAFTDDSTKLYKDLFSKADKALYLSK
                     ADGKNCYSTYE"
     repeat_region   6011..6569
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=6080..6108
                     /rpt_unit_seq="atttacataccacttagttaatataaaac"
     gene            complement(7099..7203)
                     /locus_tag="KR250_003463"
     CDS             complement(7099..7203)
                     /locus_tag="KR250_003463"
                     /inference="COORDINATES: protein motif:HMM:NF024729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBH0069348.1"
                     /translation="MDDYIYYYNNFRYQNRLNSMSPFEFRKYLSTERA"
     gene            7828..8073
                     /locus_tag="KR250_003464"
     CDS             7828..8073
                     /locus_tag="KR250_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069349.1"
                     /translation="MNQKKIKISIKMVIIGIFAVIIAFVLSRLFVEYIDELLRSNWQY
                     QLFESESSYIVFANCIMFSNILLEIFLIYICRKFRKI"
BASE COUNT         3359 a          991 c         1076 g         2921 t
ORIGIN      
        1 tatccatctc tatgtaatat ttttcatatt aaaactgatt tctatagatt aatattttat
       61 aaatttagta aatgaatatt tattatagtc tttaacaaaa aatacaagaa taaacttaat
      121 tttaataact aaaattaatt atgcaaaatt attaatatgg tattaaatta tgtataaaaa
      181 gttaattatg aataaaataa ttatgccttt gaaaaatcat taaacttaaa attaattata
      241 aaaaaactct accttactaa ctatacagct cacaatctat ctatttatga aaaaatatga
      301 tataattgca ttcaaaaagg aaatctttca atatactaga atttaagcat taactaattt
      361 tatctaatat tattactaaa ctaaaaaata tttgtatttg tagcattgga gcaagtaact
      421 taatgtatat tcattttaaa tacttattta aaatttagca tttaaattat ttacaaatta
      481 acatttaaat acttctagca taaatacagt tgtcattaaa atttaaagta tcttatggag
      541 gaataccatg caaaatcata attatataaa aatgttaaat aaaaaggtag aagtattatc
      601 taaggaatta gatagtagaa attttaaatt aaaaacctta aatgaaatag attttttttc
      661 taaaatacca aacagagaat ttatgtacca acatattgat gactgtattt cgaaaaatat
      721 acatatagga atcatgttat taaatatcga taactttaaa ttatttaatg atatatatgg
      781 atacctagtc ggagaaaatt tattaattac tctaactcaa aacctattgc actttattcc
      841 agaatcaaag ggaatagtcg cttatttaaa aggagaaggt tttttaattg tacttccaaa
      901 tgtatcaaat aaagagttaa ataatatggg tgcaactatt atagagagcg cccgagaatt
      961 aaaaataaaa cttcctaatg aaaaagaaat atactccaat gttacattta gtatcggcag
     1021 cactatctgg aatggtgaac caaacatgac tactttgatg cttttcaacc aagtatatag
     1081 tgctttaaaa aaggctaaaa gtgaaggtaa aaatagatac attctatttg attttaaaga
     1141 taatagtttt tacaataaat tagaaaatga taaggagttc ttatcagaaa tatcacatgt
     1201 tctacaaact aaacaaattg aagtatttta ccaaccactt tataatataa aaaataatac
     1261 tatagttgga tgtgaggcac ttgcaagatg gagacaccct attaaaggac ttcttactcc
     1321 agataaattt attcctttat ttgaacagtt tggtctgata acggaattag atttgcattt
     1381 atttgaagag aactgtaaaa atataagaag atggattgat aacaatgatg tttttgttcc
     1441 aatttgttgt aacttttctc gtcttcattt ttttaaccct aattttccaa gtatgctaaa
     1501 aggtattact gaaaagtaca gcgtctctac atcaaatttt agcattgaaa taacagaaaa
     1561 tatattaata gaaaatacaa atacaattat tcaacaacta gctgaacttc gttcttatgg
     1621 tttttctgta tcaatggacg attttggttc tggatattca tcttttggta tgttgcaaaa
     1681 tctacctttt gatataatca aaattgatag gatattttta tctcataatt taaatgattt
     1741 taaaaataca accattttgt actctataat aaacattgca agagctcttg ggatgcttac
     1801 agtatgtgaa ggtgttgaaa cagaacaaca tgtagaattt attaaaagta taaattgtga
     1861 tatcgcccaa ggttattatt atgcaaaacc catggagtgc aaagaatttg aaaaattact
     1921 atctaaagag aataattcag agaaaatgtt tataaaatat tctgatatag gaaaatcttt
     1981 aatagagaag atatttgatg gattttttat tatgcaagat atgaaaaaac taagagctaa
     2041 tgtcactgaa gacattcaat ggtttgattt ttacaacaaa actgaaatta catcttttcc
     2101 taaagtaata gagcattttg aaaaacatat taaaggtaaa aaattttcta ttctttacaa
     2161 aagtataacc ccacataata ctgaatcaga ttcattatta attagtggag aagccgtttt
     2221 atcagatgaa gcttctgaac atcaaaaata tagcaatttc tactttagtg caaattgtat
     2281 aatttcagaa aataaattac tcttagcaaa attacatttg gatttaatta aaactgctgg
     2341 ctatataaat gaatttgaat catattcaat acctaaaatg acaaatacac atcttgatga
     2401 aagtaatatt ttatcacaat tttattcaat gttgcctgta ggtataattc gctacgattt
     2461 atttggtgat atgattatta cttatatgaa tgaagaaatg tttgatatta taggttatac
     2521 aaaagaacag ttttttggtg agatgggcgg aaatttaaga gcaattgttc atccagatga
     2581 tttagacttt gtttataaaa aaagtttgga tatgattgac acaaataaaa ttgaaccatt
     2641 tttatataga ttcataaaac gagatggtca aattgtcaca gttatgtata cacaatgtaa
     2701 cctttctgca attgatggaa ggtcgattgc ccagggtatg tatgtagatg taaataaaat
     2761 aaaaaattta acaaaaatct agtacaaagt attttatttt ggagaaatta atatgtttaa
     2821 agaaattttt ttaagaactt ttccaggatt tctaattata agagattcta actacagaat
     2881 tatatttata aatgataatt taaaaaattt aattaaaagt tatatgcaag ataatccact
     2941 tggaatgact aatatagaaa tagctaagaa actccctgat aatattgcta aattttttac
     3001 ggattctcac aatattcaat tagattggga aaaaaattat ccttatgata aaatttcaaa
     3061 ttggatttta gagtttaaaa aagatactac aagctattgg aatgttttag aatataaagt
     3121 agatgttgat gaaaaaacct acataattac tatggccaat gatataacaa aactctatga
     3181 agaaaataag agaaatcttc actactcaat tactgaccca ttaactggag catataatag
     3241 aaaatattta aatgatagat ttgacatatt tataggagat tatattgtac taattgactt
     3301 agataatttc aaaatgataa atgactatga aggtcataat gtaggagaca agattttatg
     3361 tgattttgtt tcacttttaa ataaagaatt aataaattct acttctatta taagattagg
     3421 aggagatgaa tttattgtta tattctctag tgatgttgac aaaaattatg tttattcaca
     3481 actagaagcc ttaagagaaa attttttaaa agtattctct aagtataagt atctttcatt
     3541 tagttatggt gttgatacag taaaaagaaa tttaaaattg acaatagttg aattggataa
     3601 aaaaatgtat aaaaacaaga aaaaatttga taaaaatgat tattaatatt gttattttaa
     3661 aataaattaa aattatcttt ctttttttaa acttgtacat tagtttataa atatgtatct
     3721 atacaagata ttgacattaa gtactgaagc atatattgtt tcagttcttt tttgttaaaa
     3781 ttaacttatt taacaaagat atcatagttt aacaaagctt ttaatttaaa tatattgatg
     3841 tataatataa ttaaaatata atagcaaaaa gaaaggagga atatcatgag catactccta
     3901 aaaaaagcac ccaaattagc aaaacatatt ataacaagtt tttatataaa tcgggatatt
     3961 gatgaagtat taaaatacct ttgtgaaaat gttacatgga tcggaccagg agaacaggaa
     4021 tttctcactt cttttaatga aataaaaaat tatttttatg caggtcaaga tgaaattcct
     4081 tcttgtgata taaataatga tatatttgaa actgtaagtg aagatgagaa tagatgtatg
     4141 gtattaggaa gatatactgt tcgaactaaa gaaaatactc aaatgatttt agaggtaaat
     4201 caacgttgta catttgagat aattgaggat agaggaaaat tattagtaaa gcacatgcac
     4261 atatcaaacc cttatggtga aatgcaactt gatgaatact ttcccactaa aattggaact
     4321 caaagttatg attatttgca aagattacta aaggaaaaaa cagaagttat tgaaatgatt
     4381 acaaataata tcaatggtgg cttaaaaggt agcaatgatg ataatacata ctcatttttc
     4441 tatgtcaatg aaggattgcc caaaatatta ggatacacat atgatgagtt catggaaatg
     4501 agtggtggtt cagcagtggg tgctgtatat ccaccagact tacctaaagc actagaagat
     4561 tgtgaacgat gttttgcaaa aggacctaca tattcatctg aatatagaat ccgaaaaaaa
     4621 gatggaacat taatgtgggt attagattct ggaatgaaat cattaaatag tgatggcata
     4681 gtaaaaatta atagtattat tactgacata acccaactaa aaaacataga atctgaacta
     4741 aaattagaaa gagagcgata tcgtattgct ttgcaaaata ttacagatat catgtttgag
     4801 tatgatattg aaaacgatac ccttataaaa tatcaacgtg tagaaattga caaaaaaatt
     4861 gaattagaaa attttgaaac aaaaaattac tccaaactat tagaatctgg taaaattatt
     4921 caccttgatg atattggaaa actactagaa gtacttcatg gaaatcttcg tgaaacaatt
     4981 gagataagac aagtaaattc attaactaaa aatgaatgga gatggattcg tgttcaatgt
     5041 tcagttatat atgatagtga ccataaccct atcaaaacaa taggcgtttt aaaagacata
     5101 actgaagata aatctaaatt agaaagctta attaatcagg ctcaacgtga cccactaaca
     5161 caattatata atcaaagagt atcacaaaat cttattgaag agtatctatg tagttctgat
     5221 tccaaaaata atgatgctct attagttatt gatattgatg attttaaaac tgtaaatgat
     5281 acttttgggc atttagaggg aaatgaagtt ttagttgctg tatcaaaaat actattacat
     5341 aacacacatg ataaagacat agttgctcga attggtggag atgaatttac aatatttata
     5401 aaatcattaa caaaggattt aataataaaa attactaatg atattttaaa tgatgcaagt
     5461 aaaataaaag taaaggacaa tcataaaata acattaagta ttggtattgc cttcactgat
     5521 gattcaacta aattatataa agatttattt tctaaagctg ataaagctct ttatctatca
     5581 aaagcagatg gcaaaaattg ttatagtaca tacgaataat aaagctattt atacgaccaa
     5641 gaatgaacct ttaaatttaa tttcatagtc taacaatcaa agagttgaag ctaatcttat
     5701 agctagtcaa ctagttccta gaaaaaattt aaacaatata tttattgata ctgctcattt
     5761 ttctaaagag caaatttata taaaaaatta aacttttatg tacatacaga tactaatgat
     5821 aaacagttat cattagtatc ttctatctta aaacaaaatg ataatttatc tatataaaaa
     5881 agtgctttgt tgacagagtt cactttttta tataggtaca gaatttaagt ataaatacat
     5941 gttttattct atacatttta gttaatgtaa aatttcttga ttgtactctc taacttttca
     6001 acattatact atttatatac cacttagtta atataaaccc cttagctggt tctagtgctt
     6061 tttctatgat tttagtcata tttacatacc acttagttaa tataaaacca atttagatga
     6121 tgtatatgtg caaaaggaag aaataattta cataccactt agttaatata aaacgctact
     6181 aaacttgata tacatgacca ttataataat aaatttacat accacttagt taatataaaa
     6241 cttccataaa aacatcccct ttttattctt cttccttata tttacatacc acttagttaa
     6301 tataaaacag tcttttttta atgcagagat gcaagaagtt ttataattta cataccactt
     6361 acttaatata aaactatcag tatttaagta ataatttaag taatatagga tttacatacc
     6421 acttagttaa tataaaacgg tcactacaac ttatacttcc agtctaacga tttgaattta
     6481 cataccactt agttaatata aaacatattt tggatttatt aatataaaca taattaaaaa
     6541 atttacatac cacttagtta atataaaacc ccaaaataaa ctgggcattt tcaatactta
     6601 cacatatgca actctcttaa atttgcagtg aaccatgagt agtgcaaatg ataaagctta
     6661 tcatataccc tcaacacctt atatttcaag cgttaagctc tactttgtaa caaatatcgc
     6721 tcactgcaaa actatttaca tttttattat atcataaata tattattttt gaatatctat
     6781 accaatttgt ggtataataa aagcaagaag aactacaatc tatttagacg tagagtgaag
     6841 ttcaaatact aatgtttatt ctttttgaac ttgattttta gttcaaaact aaagtcactc
     6901 ctgcctgtgt gaactatacc cgctgtttta tacagttaaa acatgtaaaa atagttataa
     6961 agtactagtg taactcatta gtactctttt tattatattc atataattaa tatctatatt
     7021 ttaaagttta ctatctaaaa aagagcacgt caaataaaac cctagatatt taattttttt
     7081 agagatactt aaaataaatt atgctctctc tgtacttaaa tatttcctaa attcaaatgg
     7141 agacatagaa tttaatctat tttgatatct aaaattatta tagtagtata tataatcatc
     7201 tattgcttta actaattatt catatgtatc aaatttcctt aaatagtaca tttctacctt
     7261 taaaattccc caaaatcctt ccataggagc attgtcaagg caacgaccaa ctcttgtata
     7321 atatatgtat atctaacacc attaagaaat atatatttta taatatatat ttcttcattt
     7381 aatgcactta gaaaagcaaa gaaaatacag attagtacac tttttagaga aaaataaaat
     7441 taatacatct aaaatattaa aaaatacttt agataatcta aagacacatc tcaaagtttt
     7501 taactaaatt ataaaactaa ttacaaatgc tatgtaacga taataaataa aaaatttact
     7561 tcgcttaagc gacgtgtcgt tgaaatattt catgttgcca actggttcgt aagttgataa
     7621 gaaaagaaca ctaattattt agagttcttt tcttagctac aagtggatta taatactttt
     7681 aaatctgtga gaaaatcata tataataatg atatgaacaa gtaaagaaaa tacaaaaatg
     7741 acaaaattat aaataaaata ttataaaatc ataaggtcat aattgttata ttgtagaaac
     7801 aaagtaaaga gagagggtgg agaataaatg aatcaaaaaa aaataaagat tagtattaaa
     7861 atggtaatta taggtatatt tgctgtaatt attgcttttg tattatccag attattcgtt
     7921 gagtatattg atgaattatt aaggtctaat tggcaatatc agctatttga aagtgagagt
     7981 tcatatatag tatttgccaa ttgtattatg ttctcgaata tattattaga aatattttta
     8041 atatatattt gtagaaaatt tagaaaaatc taatatggtc aacgtatttc gaatcgaata
     8101 cttcatgatt atttagtcta ttgtgctagt taaagtttaa ttcccatata aagtaaattg
     8161 attttagtaa aacaaatcac ccctaagggt tagtttatgg ataagtcatc caaatattaa
     8221 ctctttaatt tttgctatag attcatcttt gctcataact ttacttataa aaaatgaaat
     8281 attataagct agaactttta ttctaattct agtcataaat cctagcatag agttactttt
     8341 tttatat
//
LOCUS       DAEPIN010000115         8254 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.117, whole genome shotgun sequence.
ACCESSION   DAEPIN010000115 DAEPIN010000000
VERSION     DAEPIN010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8254
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.117"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            complement(122..517)
                     /locus_tag="KR250_003465"
     CDS             complement(122..517)
                     /locus_tag="KR250_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0069350.1"
                     /translation="MNRIKELREEKGISLDKLSEDLHINKSTLSRIENGLREPKKSTI
                     EEYANYFDVSTDYLLGRTDVRNSLFINKNEKDYDAENFKTEKELIENMYLDEDMKEVF
                     NIFSELSPDAREKALKVAELFLLDEKNKK"
     gene            696..929
                     /locus_tag="KR250_003466"
     CDS             696..929
                     /locus_tag="KR250_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF739 family protein"
                     /protein_id="HBH0069351.1"
                     /translation="MYLNRLEGLMKENRHTQKNVADILGLSSYGFRLKLKGKNEFKAS
                     EIKKISKLYNVSADYFFSDEVAKIAIKEERGNK"
     gene            929..1681
                     /locus_tag="KR250_003467"
     CDS             929..1681
                     /locus_tag="KR250_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HBH0069352.1"
                     /translation="MKNLTIIKQNNQFLVESREVAELIEKKHDNLLRDIRGYKKILED
                     SSNLKSQDFFIESTYINTQNKIQPCYLLTKKGCDMVANKMTGEKGIIFTAIYVTKFEE
                     MERELKEQQPKLPTTYKEALQQLLIEVEEKEQLQLENQVMKPKADYFDALVERNLLTN
                     IRDTAKELGVKEKTFVLWLIEKKYCYRDLKGKIKPYSNKMQYFELKEFTTPYGHSDTQ
                     TLINPKGREAFRLLLIKDGLVKEKEYQITLLG"
     gene            1726..1980
                     /locus_tag="KR250_003468"
     CDS             1726..1980
                     /locus_tag="KR250_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069353.1"
                     /translation="MSEDIFYGIKNTLDEIAEVQIKNKQGVLKEVGMLISGEDNSCIT
                     HCLDEDLIKFYIKEEAVLTIDKDSHLLCMLDALFYNFLDK"
     gene            2008..2451
                     /locus_tag="KR250_003469"
     CDS             2008..2451
                     /locus_tag="KR250_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH0069354.1"
                     /translation="MKKLGNISNFNLDKQEDKSFNDLDNISICFSEGIRKVVEMNLNN
                     YKNKISKYLNETSKIELLEPKELTIVISKGYPDYLMSVEEASKRLKIDKVFGYELIKN
                     GLLKSVDIGATKVSSYELDDFITRNQGKNIKEMLREMKELREGVI"
     gene            2579..2767
                     /locus_tag="KR250_003470"
     CDS             2579..2767
                     /locus_tag="KR250_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069355.1"
                     /translation="MKIIYKNKVYKVEQDKKLFRITYYDEQRGSKKFNKEKKIKRSVL
                     TRDIELVNLYLPAHLKIK"
     gene            complement(2902..3072)
                     /locus_tag="KR250_003471"
     CDS             complement(2902..3072)
                     /locus_tag="KR250_003471"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069356.1"
                     /translation="MSNKDIYTREEDKRFTLRINKLLFEKIEQLAQKDKRSIGREIEF
                     ILEKYFEDNPLE"
     gene            3215..3373
                     /locus_tag="KR250_003472"
     CDS             3215..3373
                     /locus_tag="KR250_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HBH0069357.1"
                     /translation="MTNERVRFTFRLPAPLLEKIKNRASIEGSSMNSLILHILWDYIK
                     EIENKEVK"
     gene            3373..3600
                     /locus_tag="KR250_003473"
     CDS             3373..3600
                     /locus_tag="KR250_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069358.1"
                     /translation="MVELVKEFDLQTIKVGNAVKVNCKRFGFEIDCIVVVATEKELNL
                     AYFDEGRGCMEYQALITEDIQDGDYEIKILS"
     gene            3698..4012
                     /locus_tag="KR250_003474"
     CDS             3698..4012
                     /locus_tag="KR250_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069359.1"
                     /translation="MESLKRIKKMVQKQLVLAELEINKNSKLYEELENKDRGLIDDIH
                     MREYLREKVAWERVKYAIENILGGINLEIKSKEHEESEDYKIFQLILEELERDKPIDV
                     QI"
     gene            4096..4605
                     /locus_tag="KR250_003475"
     CDS             4096..4605
                     /locus_tag="KR250_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siphovirus Gp157 family protein"
                     /protein_id="HBH0069360.1"
                     /translation="MSTLYELTTDLLEIEEGLTETTGNETEKLEEIKEIIKQEIQNKN
                     TRIVSVILNIDSDINSIDSEIKRLQELKRVKKNTLDRLKSNIKDCMELLGTKKVETVL
                     GNISIRKSAGSLVIEDEEKIPAIYKTVEQVVKVDKNIIKDFIKKGHEVEGCRIEYGTT
                     LTIPKAKKE"
     gene            4615..5223
                     /locus_tag="KR250_003476"
     CDS             4615..5223
                     /locus_tag="KR250_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ERF family protein"
                     /protein_id="HBH0069361.1"
                     /translation="METNNVYIKLVNIQSTLKAPKSQFNSFGKYNYRSCEDILEGLKP
                     ILKEEKALVILDDNIVQIGNRFYVEATATLIDAETGEKVSTKALAREDETKKGMDLAQ
                     VTGSVSSYARKYALNGLFCIDDTKDSDATNKHGNEQKKKEVNESELNTLYSLGESIEK
                     DKNRVDSEVYKKFGKLAVDLTKQEYEKVLNGYKSILEKQKQE"
     gene            5233..6114
                     /locus_tag="KR250_003477"
     CDS             5233..6114
                     /locus_tag="KR250_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HBH0069362.1"
                     /translation="MGIIRVSKDKDNPYVVLNKTCLEDVKLSWQAKGLHSYLISKPDH
                     WKIYVNDLCKRSKNGRDATANILKELIENGYITRKPCRDSNTNKMLGGYDYEVYEIPL
                     ENPQKLKSRKTDFPETGFPGNRVSRKPENTEVVSNDFKVNNDITTIVINEQSNKDKTT
                     YIKKYFEKYIGVITPNNFIELMSYLDDGMEADVIIRAVDEAVGSGVKNYKYVKTILNN
                     WIEAGVKTVLELTEYQNEFERKKKSKQEKKQSNSKTVNTHNVNKNKFANFNQTFTQYE
                     EKELDEIIKKSQKEKFK"
     gene            6175..6348
                     /locus_tag="KR250_003478"
     CDS             6175..6348
                     /locus_tag="KR250_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HBH0069363.1"
                     /translation="MARIYAQRSGSLNEQDRLELLRLLGKAGYTVKVAREKQNSKTTY
                     TYFVEYTEEQEEK"
     gene            6364..6771
                     /locus_tag="KR250_003479"
     CDS             6364..6771
                     /locus_tag="KR250_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBH0069364.1"
                     /translation="MNTITLVGRLVADAELKYLPNSGTPKITFSMAVDRRFKDKNGNK
                     ITDFIQCEQLGKHVENLVQYLVKGKPIYAVGELNIYNYKDENGCWKSITKVNVNALEL
                     LSSKNDNNAKQEYVPPGLDPQGFQAIDDDDIPF"
     gene            6845..7039
                     /locus_tag="KR250_003480"
                     /pseudo
     CDS             6845..7039
                     /locus_tag="KR250_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861747.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            7091..7348
                     /locus_tag="KR250_003481"
     CDS             7091..7348
                     /locus_tag="KR250_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069365.1"
                     /translation="MIIIRSQDKTDLVKADWINVDKECVYAMFGDMDNFKKIGEYEDE
                     KRAIQVLDNIQRFIEGGTRTDSIDSYKVRSYRNRIFQMPVK"
     gene            7362..7727
                     /locus_tag="KR250_003482"
     CDS             7362..7727
                     /locus_tag="KR250_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069366.1"
                     /translation="MLKVEKYFSGSLGTNIIDDDPTCRNYLALYCCVHGVRKNGELIF
                     PTCEKMLIEFNVDKNRKKKKKGSKVKLINAKTGEEKIFDSIDSAACFLRLQSQAVYQT
                     IKKKTKTRSGWKAEYIEEE"
     gene            7845..8180
                     /locus_tag="KR250_003483"
     CDS             7845..8180
                     /locus_tag="KR250_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015981539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069367.1"
                     /translation="MNILASVILVIGSFIAGRVYEYRLNLNENDEADSKVLLDVFNEI
                     SVLREENKSLKEKLQEKELLFINRLIDFLHDKKICECCIYDCKIDDIEYDCEDGIKKW
                     LDSEELIFE"
BASE COUNT         3520 a          804 c         1504 g         2426 t
ORIGIN      
        1 gttgaaagaa ttggataaaa ataaaatcaa tgaatataaa ataaaagtga aagaaataca
       61 taaaatcaat aaaaaagagg aatgattcct cttttttatt atattcattt tcagaaataa
      121 actatttttt atttttttcg tctagtaaaa ataattctgc aacttttaat gctttttctc
      181 ttgcgtctgg gcttagctcg ctaaatatat taaaaacttc tttcatatct tcgtcaagat
      241 acatattctc aataagctcc ttttctgttt taaaattttc agcatcataa tctttttcgt
      301 ttttatttat aaataagcta tttctaacat cagttcttcc taataaataa tctgtagaca
      361 catcaaaata gtttgcatat tcttctattg tacttttctt aggttctctt aaaccatttt
      421 ctatccttga tagtgtagat ttgtttatat gtaaatcctc gcttaatttg tctagcgaga
      481 tgcctttttc ttctctcaat tcttttatcc tattcaattt gcacaacccc tttttatatt
      541 aattccaaaa tagcaacttt tatttatatt atagcaacaa aattaattat tacaattttt
      601 tttgctaaaa aagcaacaaa agtattgact ataatcacaa aacttgatat tatataaata
      661 tgaagttgcc aaaatagcaa aaaggaagtg atttaatgta cttaaataga ttagaaggat
      721 tgatgaagga aaatagacac acccaaaaaa atgtggcaga tatattaggt cttagctcat
      781 atggttttag attaaaatta aaaggaaaaa acgaatttaa agcaagtgaa ataaaaaaga
      841 tatctaaatt atataatgta tctgcggatt attttttttc agatgaagtt gctaaaatag
      901 caataaaaga agaaaggggc aataaataat gaagaatcta accataatca agcaaaataa
      961 tcaattttta gttgaaagta gagaagtagc agaattaata gaaaagaagc acgataattt
     1021 attaagagat ataagaggat acaagaagat tttagaggac tcatcaaatt tgaagagtca
     1081 agatttcttc atagaaagta cttatataaa tactcaaaat aaaattcaac cttgctactt
     1141 attaactaaa aaaggttgtg acatggtagc taataaaatg acaggtgaaa agggaattat
     1201 atttacagcg atttatgtaa ctaagtttga agaaatggag cgagagttaa aagaacaaca
     1261 acctaaatta ccaactacat ataaagaagc actgcaacag ttattaatcg aagttgaaga
     1321 aaaagagcaa ttacaattag aaaatcaagt aatgaaacca aaagcagatt actttgatgc
     1381 tctagtagaa agaaacttgc taactaatat aagagataca gcaaaagaac ttggagtcaa
     1441 agaaaaaaca tttgttttat ggttaataga gaagaaatat tgttacagag atttaaaagg
     1501 caaaataaaa ccttactcta acaagatgca gtattttgaa ctgaaagaat ttacaacacc
     1561 atacggacat tcagatactc aaacattaat aaatccaaaa ggaagagaag catttagatt
     1621 attacttata aaggatggac tggtaaaaga aaaagaatat caaataactt tattaggcta
     1681 aaattgaaag tactttgaaa attaaagatt atggaggtag ttacaatgag cgaagacata
     1741 ttttatggta taaaaaatac tcttgatgaa atagcagagg ttcaaattaa aaataaacaa
     1801 ggtgtgttaa aagaagttgg aatgttaata agtggtgagg acaattcttg tataacgcat
     1861 tgtttagatg aagatttaat taagttttac ataaaagaag aggcagtatt gacaatagat
     1921 aaagatagtc atttgttatg tatgcttgat gctctatttt ataactttct tgataaataa
     1981 taaatacaga atattcttga aatggtggtg aagaaattgg gtaatatatc taactttaat
     2041 ttagataaac aagaagataa aagttttaat gacctggata atatatcaat ttgtttttca
     2101 gaaggtattc gtaaagttgt agaaatgaat ttaaacaact ataaaaataa aatctcaaaa
     2161 tacttaaatg aaacttcgaa aatagaattg ttggaaccaa aagaattaac aattgttata
     2221 agtaaaggtt atcccgatta tcttatgtct gttgaagaag caagtaaaag attgaaaata
     2281 gataaagtat ttggatatga gttaataaaa aatggacttt tgaagtcagt tgatataggg
     2341 gcaactaaag tttctagtta tgaattagat gattttataa ctaggaatca aggaaaaaac
     2401 atcaaagaaa tgcttagaga aatgaaagaa cttagagagg gggtgattta attggaacta
     2461 gtaacataca gaaacaagct tgttttacta aaagatggag aaaagattgc aactataagt
     2521 ttaaaaagga aatttctcag caacagactt aaattaaaaa taagatagga gagataaatt
     2581 gaaaataatt tataaaaaca aagtttacaa agtagaacaa gacaaaaagt tatttagaat
     2641 tacatactat gatgagcaga gaggtagtaa gaagtttaat aaagagaaga aaataaaaag
     2701 aagtgtttta acaagagata tagagttagt taacttgtat ttaccagcac atttaaaaat
     2761 aaaataagct ataaataatt aaagaaaaag gtgattagat ggaaatagaa caagcaacaa
     2821 tacgcctgcc tagagaactt aaagacaagc ttctaaaaca ggcaaaagtt aaaggatata
     2881 cattaaaaga tatgatagtt tttattctaa aggattatct tcaaaatatt tctcaagaat
     2941 aaattcaatt tctctaccta tagaacgctt atctttttga gcaagttgtt cgattttctc
     3001 aaaaagaagt ttattaattc ttagtgtaaa tcttttatcc tcttcacgag tataaatatc
     3061 tttattagac atatttatca ttcctcacat aaaatttgac gtctttatga cactttaata
     3121 ataaaataat ataaaagaaa agtcaacaaa aatgcttgac gtcaaatata tgacatggta
     3181 ttattaaaac aagaagggag ttgacgtcaa atatatgaca aatgaaagag ttagatttac
     3241 atttagatta ccagcaccat tgcttgaaaa aattaaaaat agagcatcaa tagaaggtag
     3301 ttcaatgaac tcacttatat tacacatact ttgggattac ataaaagaaa tagaaaataa
     3361 ggaggtcaaa taatggttga attggtaaaa gaatttgatt tacaaacaat taaagtagga
     3421 aatgcagtaa aagtaaattg caaaagattt ggttttgaaa ttgattgtat agtagtagta
     3481 gcaacagaaa aagaattaaa tttagcatac tttgatgaag gtagaggctg tatggagtat
     3541 caagccttaa taacagaaga tattcaagat ggtgattatg agattaaaat tttatcttag
     3601 gaggaaataa aatggctgct ttaataatgg taggtttatt tgcaatatgt ttagtaggat
     3661 tagttcaaaa tagagattaa attaaggggg attaattatg gaaagtttaa aaagaataaa
     3721 aaaaatggtt caaaaacaat tagttttagc tgaattagag ataaacaaaa atagcaaact
     3781 ttatgaggaa cttgaaaata aggatagagg tttaatagat gatatacaca tgagagaata
     3841 tctaagagaa aaagttgcat gggaaagagt aaaatatgct attgaaaata ttttaggtgg
     3901 tataaattta gagattaaat caaaggaaca tgaagaaagt gaggattaca agatatttca
     3961 attaatttta gaagaacttg aaagagataa acctatagat gttcagatat aagaaaagag
     4021 ccattgccgt ggctcaattc aaataaatat atcaaaattt aattaagcta attatagcac
     4081 aaacggaggg aaattatgag tactttatat gaattaacta cagatttatt agaaatagaa
     4141 gaaggtttaa cagaaacaac aggaaatgaa actgaaaaac tagaggaaat aaaagaaata
     4201 ataaaacaag agatacaaaa taaaaacact aggatagttt cagtaatatt aaacattgac
     4261 agtgatataa actctataga ttcagagatt aaaagactgc aagagttaaa aagggtcaaa
     4321 aagaatactc ttgatagatt aaaaagcaat ataaaagact gtatggaatt acttggtact
     4381 aaaaaagtag aaacagtttt aggaaatata agtataagaa agtcagcagg tagcttagtc
     4441 atagaagatg aagaaaagat acctgctata tataaaacag tagagcaagt tgtaaaagta
     4501 gataagaata tcattaaaga ctttattaaa aaaggtcatg aggttgaagg ttgcaggatt
     4561 gaatatggaa ctacactaac aattccaaaa gctaaaaaag agtaggtgag gaccatggaa
     4621 actaataatg tttatataaa acttgtaaat atacagagta ctttaaaagc tcctaaaagt
     4681 caatttaata gctttggtaa atacaactat aggagttgtg aggatatact agaaggttta
     4741 aaacctattc taaaggaaga aaaagcatta gttatattgg atgataatat tgttcagata
     4801 ggaaatagat tttatgtaga agctacagca actttaatag atgcagaaac aggagagaaa
     4861 gtatctacaa aggcattagc tagagaagat gaaactaaaa aaggtatgga tttagcacaa
     4921 gtaactggaa gtgtatcaag ttatgcaaga aagtacgctt taaatggatt attttgtatt
     4981 gatgatacaa aagatagtga tgcaacaaat aaacatggaa atgagcagaa aaaaaaagaa
     5041 gttaatgaga gtgaattaaa tacactatat tcgctaggag aatctataga aaaagataaa
     5101 aatagagttg atagtgaagt atataagaag tttggaaagt tagcagtaga tttgactaag
     5161 caagagtatg agaaagtttt aaatggatat aagagcattt tagagaagca aaaacaagag
     5221 taggtgatag tattggggat tataagagta agcaaagaca aagataatcc atatgtagtt
     5281 ttaaataaaa cttgtttgga agatgtaaaa ttaagctggc aagcaaaagg tttacattca
     5341 tatctgatta gtaagcccga ccactggaaa atctatgtta atgacctttg caagagaagt
     5401 aaaaatggaa gagatgctac agcaaatatt ttaaaagaac ttatagaaaa tggatatata
     5461 acaagaaaac cttgtagaga ttctaatact aataaaatgc ttggagggta tgattatgaa
     5521 gtatatgaga taccacttga aaatcctcag aagctaaaat cccgaaaaac tgatttcccg
     5581 gaaaccggat ttcccggaaa ccgggtttct cggaaaccgg aaaacacgga agtagtaagt
     5641 aatgacttta aagtaaataa tgatattact actattgtta ttaatgaaca atccaataaa
     5701 gacaaaacca cctatataaa aaaatacttt gaaaaatata taggtgtgat tactcctaat
     5761 aactttatag agttaatgag ttacttagat gatggaatgg aagctgatgt aataataaga
     5821 gctgttgatg aagctgtagg cagtggagtt aagaattata agtatgtaaa gacaatctta
     5881 aataattgga tagaagcagg tgtaaaaact gttttagaac ttacagaata tcagaatgaa
     5941 tttgaaagaa agaaaaagag taagcaggaa aagaagcagt ctaatagtaa aactgtgaat
     6001 actcataatg tgaataaaaa taagtttgct aacttcaatc agactttcac tcaatatgaa
     6061 gaaaaagagc tagatgagat tattaaaaag agtcagaagg aaaaatttaa ataaaattaa
     6121 acttctagga agtaaatatc aatatattgc ttcctagaaa aggggaggta taaaatggcg
     6181 agaatatatg cacaaagaag tggttcttta aatgaacaag atagattgga gttattaaga
     6241 ttacttggga aagctggata tacagtaaag gttgctagag agaagcaaaa tagcaagaca
     6301 acttatactt actttgttga gtatacagaa gaacaggaag aaaaatagaa gggggctagt
     6361 taaatgaata caataacttt agttggaaga ttagttgcag atgcagaatt gaagtacctt
     6421 ccaaattcag gtactccaaa aataaccttt tcaatggcag tagatagaag gtttaaagat
     6481 aaaaatggaa ataaaataac tgattttatt caatgcgagc aattaggaaa acatgtagag
     6541 aatttagtgc aatatcttgt taaaggtaag cctatatatg ctgttggaga gttaaatata
     6601 tataattaca aagatgaaaa tggttgctgg aaatctatta ctaaggttaa tgtaaatgct
     6661 ttagaactac tttctagtaa aaatgataat aatgctaaac aagaatatgt accaccagga
     6721 ttagacccac aaggttttca agcaatagat gatgacgata taccttttta attaagttaa
     6781 ataatctagg gagtaattat gcaatattac ttcctaggag ttaaaaaata ttggaggttt
     6841 aagagtgaaa tatgagtgtg agaaagtgtt cttagaatgc gataagggaa gttttgagat
     6901 aaatgataca agagctgaag aagtaacatt cgagggtaca gaaatagaca atctgtttga
     6961 gagagtaaaa tatgaaggga cttttacaat aatatctgga tgggattctt tattgagaga
     7021 tatattgtgg cttgaaatgc ggaaagtctt aaaaattata acaggaaaaa tgatgatggg
     7081 ggtattagga atgataataa ttagaagtca agataaaact gatttagtaa aagctgattg
     7141 gataaatgtt gataaagaat gtgtatatgc aatgtttgga gatatggata actttaaaaa
     7201 gataggtgag tatgaggatg aaaaaagggc tatacaagtg ctggataata tacaaagatt
     7261 tattgaaggt ggaactagaa cagattctat agacagttat aaggttagaa gttatagaaa
     7321 tagaatattt caaatgccag ttaaataggg aggttactga aatgttaaag gttgaaaaat
     7381 attttagtgg ttctttagga acaaatatca tagatgatga ccctacatgt agaaattact
     7441 tagcactata ttgttgtgtt cacggagtaa gaaaaaatgg agaacttata tttccaacat
     7501 gtgaaaaaat gttaatagag tttaatgttg ataaaaatag aaaaaagaaa aagaaaggtt
     7561 caaaggtaaa gttgattaat gccaaaactg gtgaagaaaa aatatttgat tctatagata
     7621 gtgcagcatg ttttttaaga ctacagagtc aggcagttta ccaaacaatt aaaaagaaaa
     7681 ctaaaacaag aagtggctgg aaagctgaat atattgagga ggaataatgg aagtttcaag
     7741 gactgaatac acaattaaaa gagcaaaaga gttatatgat aatggagagg atatatttat
     7801 tgctatagat aaggctagag aagaatatga ggagatggtt aaaagtgaat atcttagcta
     7861 gtgtgatatt agtaatagga agttttatag ctggtagagt ttatgagtat agattgaatt
     7921 taaatgaaaa tgatgaagct gattcaaaag tacttttaga tgtttttaat gaaattagtg
     7981 tgttaagaga agaaaataaa agccttaaag aaaagttaca agagaaagag ttactattca
     8041 tcaatagatt aatagatttt ttacatgata aaaagatatg cgaatgttgt atttatgact
     8101 gtaagattga tgatattgaa tatgattgtg aagatggtat taaaaagtgg cttgatagcg
     8161 aagaacttat atttgaatag aagaaatatc taattaaaac agtttagaga gttgcaaaat
     8221 gttttttaat aaaattatca ttgagatgtt ttgt
//
LOCUS       DAEPIN010000116         8203 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.118, whole genome shotgun sequence.
ACCESSION   DAEPIN010000116 DAEPIN010000000
VERSION     DAEPIN010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8203
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.118"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     repeat_region   160..516
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=160..188
                     /rpt_unit_seq="gttttagattaactatatggaatgtaaat"
     gene            678..866
                     /locus_tag="KR250_003484"
     CDS             678..866
                     /locus_tag="KR250_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069368.1"
                     /translation="MKIITDSSRADYFKQRRQNKKTFSVLLDREKVEKIEEHLKKQNK
                     TKTIWLEEKINEELEKEE"
     gene            985..1851
                     /locus_tag="KR250_003485"
     CDS             985..1851
                     /locus_tag="KR250_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
                     /protein_id="HBH0069369.1"
                     /translation="MENLIVKEFNGSQIYTFMWKEKSCWIANQIVGLFDYADVSKTIQ
                     DCIKAEDFEIEQEYDVLKGNEFNDFVTTLNVVANNIISNKARSITIFYEDGLYGFLQY
                     TDKPIGVQFRKWLRREVLPSIRQTGAYITNNADPQALRDKANEIESLDTVNKTIEILT
                     PFLDNAGIDEKAKLLTAKTIYKKAGIELPLEIEEKEHFFDTVQIATKLNVYSKSNKPA
                     FHAIGEIIKKLDIQDNEKLVVLESKRGWSGSVNKYSQSVIDKVRNWIEENNRPTKIAG
                     EKKNYHVVYKIE"
     gene            1904..2059
                     /locus_tag="KR250_003486"
     CDS             1904..2059
                     /locus_tag="KR250_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069370.1"
                     /translation="MHENLLDIDRIELIKELGNIFEKMKNENPDEFYRFVSLVKEECR
                     KKEEKNK"
     gene            complement(2478..2690)
                     /locus_tag="KR250_003487"
     CDS             complement(2478..2690)
                     /locus_tag="KR250_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010249169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0069371.1"
                     /translation="MSIKFFKLIDLLNRRGITKEELRLKIGASSTTIAKLSSNKNVSL
                     DVIDKICKALNCQPGDIMEYEDDEKL"
     gene            2965..3309
                     /locus_tag="KR250_003488"
                     /pseudo
     CDS             2965..3309
                     /locus_tag="KR250_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012103644.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
     gene            3391..3744
                     /locus_tag="KR250_003489"
     CDS             3391..3744
                     /locus_tag="KR250_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069372.1"
                     /translation="MQFLVAKTFFKKAGVDIPLEIEEKEHFYDTKQIARKLGLYTMSN
                     KPAFMAVKQIIRKLDIKEDEMKTVLETNGNWTGTVTKYSNKVTEKVKVWLKENNNPIS
                     IKGDKKNYYILYKNN"
     gene            3777..3929
                     /locus_tag="KR250_003490"
     CDS             3777..3929
                     /locus_tag="KR250_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069373.1"
                     /translation="MYENLLDMDRIELIRELGSIFEKMKNENPDEFYRFVSLVKEECR
                     KKRERE"
     repeat_region   4223..4783
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=4223..4252
                     /rpt_unit_seq="gttgaagaataacatgagatgtttttaaat"
     gene            4906..5433
                     /locus_tag="KR250_003491"
     CDS             4906..5433
                     /locus_tag="KR250_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HBH0069374.1"
                     /translation="MREGKRRRGCLFWFILIILFSGVVGAIAGNSTNNESTEKQKEDL
                     TKYIGEEGSIGDLKLTVNSISKASEISVASGYLAYTPDSGKYGIINLTIKNQTKESQS
                     FMLNSFTLIGPDDSKYVPSLLIDVGNKYITMDTVNPNLDVTGNIAFEIPKDLLVSDCK
                     LKYSGSEQEFILKEK"
     gene            5572..6339
                     /locus_tag="KR250_003492"
     CDS             5572..6339
                     /locus_tag="KR250_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4428 domain-containing protein"
                     /protein_id="HBH0069375.1"
                     /translation="MGLFGKKKADICCICNTEIGVLNIEDGWICNSCFKEYCDALSMT
                     KAPKILRKLDIEKTISSTKKNNELQKIFNTTNNIENYIEFDEDNKKWFVSKKSINDKK
                     TPIIHSYEDIVEFELLENGETVTKGGIGRALAGGILFGEAGAIVGGITGKKTTRKVVD
                     TFKIKITINNIDNPIEYIELINKKTKTNSSAYEKAYKDAHKILSILSAITQSIKETDN
                     INTKSVADEILKYKNLLDMEAITQDEFNTKKKELLNL"
     gene            6405..>8203
                     /locus_tag="KR250_003493"
     CDS             6405..>8203
                     /locus_tag="KR250_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HBH0069376.1"
                     /translation="MATIQTSIKIFDGMTPAFRNMTNSINTTINSLERLQGRLNNPLN
                     AGNIQASQQSLNNIESILTRIEQKIGRNTNEQENFNNKIRQGSEAGSLLVSKLKSLAG
                     IYIGIRGIESITKAADTIASTKARLNLMNDGLQTTEQLNKMIYLSAQSARASYADTAA
                     QVSKLGILAGEAFGSSAEVVKFAELMNKSFVIGGTSASEASAAMYQLTQAMVAGKLQG
                     DEFRSIMENAPLLATKIAESMGKGKEALKDLSRTGAITADVVRNALFKASAEIEKKFE
                     SMPITFSQALTMMKNDAYMIFGQTLGKISGALQSVRFSEIVVSIRNVMIAISSNIYDT
                     LNIIKNILNSEFFSNLVQGFTFCVVVITKSLGSIVNTALNIVNIFAQNWSIIKPIVFG
                     VASVFILFRGVLLATKIATIGNVIANVAHAASSSLSALMTNIQAAALMRSNGATLSAT
                     IATWGLNAALLACPITWVVLGILAFVVVVFVAVAAVNKFAGTSLTALGVIVGAVFAAV
                     AAIQNVMIWLLNGCIAVNEGITNGWNQCVYLMKQAIAKGVIFIIEKMASLNDSVNNAG
                     NALGKAFVAGANIAIRGVNKLIDLLNKIPGVNIG"
BASE COUNT         3242 a          875 c         1458 g         2628 t
ORIGIN      
        1 tgcagtgagc gatatttgtt acaaaatatg gtctaacact tgaaatacaa ggcattgagg
       61 gtgtgtgata aatgttatca attgcactac tcatggttca ctgcaaattt gagagagatg
      121 cgtatgtgta ggtattggaa atgctaagtt tattttgggg ttttagatta actatatgga
      181 atgtaaatta aatatagctt cttctccata actataagtt gcatgtttta gattaactat
      241 atggaatgta aatccaaacg aatttgtatt actaaaatca ttccacaact gttttagatt
      301 aactatatgg aatgtaaatt agcttgatag tatccaccct ccatggcatt gtttgagttt
      361 tagattaact atatggaatg taaattcaga aacaggaaca tcaaaaggag gtttaaaatc
      421 tggttttaga ttaactatat ggaatgtaaa ttgctgtgat ttcactaata aaacaattaa
      481 taattgtgtt atagattaac tatgtggaga aaaactaaat aaacaaagaa agcacttaca
      541 aatatgtagg tgcttttgtt ttgctcaaat tggtcggttg agtgaaataa ttagaaaaaa
      601 ttagtaaaaa ctagtaaaaa ctcttgaaaa gtgtcgcgat acaatgtata attatattat
      661 cgcgatacag aaaagaggtg aaaattatta ctgatagcag tagagcagat tacttcaagc
      721 agagacgaca gaataagaaa acttttagtg ttctactaga tagagagaaa gtagaaaaaa
      781 ttgaagaaca tttaaaaaag cagaacaaga ctaaaactat ttggcttgaa gaaaagatta
      841 atgaagagtt agaaaaagag gaataaaaaa taagagacgt tctccccgac caaagattga
      901 acatccctta ttgacgtata ttatatacac taactatagt atacgtcatt ccttaaaaaa
      961 attcaattaa ggagtgtaat agttatggaa aatttaatag taaaagagtt taatggaagt
     1021 caaatttata cttttatgtg gaaagaaaaa tcttgttgga tagctaatca aatagttgga
     1081 ttattcgatt atgctgatgt atctaaaaca atacaggatt gcataaaagc agaagacttt
     1141 gagattgaac aagagtatga tgtattgaaa ggaaatgaat ttaatgattt tgtaactact
     1201 ttaaatgtag tcgcaaataa tataattagt aataaagcta gaagtataac tattttttat
     1261 gaagatggtt tgtatggatt tttacaatac acagataaac caattggtgt acagtttaga
     1321 aaatggctta gacgagaagt tttaccaagc ataagacaaa ctggtgcata cataacaaat
     1381 aacgctgacc ctcaagcact acgagacaag gcaaatgaaa tagaaagtct agatacagtt
     1441 aacaagacta tagaaatact aacaccattc ttagataatg ctggaataga tgaaaaagca
     1501 aagttactta cagcaaagac tatctacaaa aaggcaggaa tagagttacc tcttgaaata
     1561 gaagagaagg aacatttctt tgatactgta caaatagcaa ctaagttaaa tgtttattct
     1621 aagtctaata aacctgcatt tcatgcaata ggtgaaatta ttaagaagtt agatatacaa
     1681 gataatgaaa agttagtagt actagagagt aaaagaggtt ggagtggaag tgttaataag
     1741 tattcacaga gtgtaataga taaagtgaga aattggatag aggagaataa tagacctact
     1801 aagattgcag gtgagaagaa gaattatcat gtagtttata agattgagta aatttataag
     1861 attgtattaa ataatttagt ttagttttgg ggggattaat acaatgcatg agaatttact
     1921 tgatatagat agaatagaac ttatcaaaga acttggaaat atctttgaaa aaatgaaaaa
     1981 tgaaaatccg gatgaatttt atagatttgt aagtttagtg aaagaagagt gtaggaaaaa
     2041 agaagagaaa aataaataaa tagacaaata aagcacttga atattttact gtttcaagtg
     2101 ctttatgtag taaaaaatga tataatatag gtaggagtta tattaactaa gtggtatgta
     2161 aatgctgtat cctttgcccc ttttgctgcg tcactcaagg tttttatatt aacaatgtgg
     2221 tatgtaaagt cgtttatcat tgctagcctc gcattcgtac tggtcaattt tatattaact
     2281 aagtggtatg taaatcattc actacaaaat atatattcta atgtatcatt cgttttatat
     2341 taactatatg gtatgtaaac aatatatata gcaaatctgt tatattaact atgaagggtt
     2401 ttatattaac tatgtggact taaaattaaa tagaaaaaag aaagcactta cttaaatagt
     2461 aggtgctttt gttttgctta taacttttca tcatcttcat attccataat atcacctggt
     2521 tggcagttta aagctttaca tattttatca attacatcaa gtgaaacatt tttatttgaa
     2581 gatagtttgg ctattgtagt agaagaagct cctattttga gtcttagttc ttctttagtt
     2641 atacctcttc tgtttagtaa gtcaattagt ttaaaaaatt ttatagacat gttaatacct
     2701 cctatatatt tatataatac tatatatatt tattaaagta aagatatatt tattaaaata
     2761 aataaaaagt attgacatta tttactaaat aatgtattat tagagtataa agataaagaa
     2821 atctttataa agataaagca ataagggata aaataaggaa tattctctct cgtcaaagtt
     2881 taaatattcc ttatgacata taactaatat gtaatataat tatatgtcat tccttaaaaa
     2941 aaatcaatta aggagtgtat ttttatgaac aatttagtat taattaacaa ccaagagttg
     3001 caggtcaagg agtttaataa tcaaagagta gttacattta aagaaattga ccaagtacat
     3061 gaaagaacag aaggtacagc aaagagaaat ttctcagaga atagaaaaca ttttatagag
     3121 aacgaagatt tcttttttgt aaaagcaagt gattttgaaa agtacggaaa tcgtacttta
     3181 gaaattccta atcgtggatt aactttaata actgaaacag gctatttgat gttagtaaaa
     3241 tcttttacag atgacttagc atggaaagta caaagacaat tagtaaatag ttattttaga
     3301 gttaaagaag aaataaaaac attagaagca gtaaatgagt caattaaatt aataactcca
     3361 ttttttgaag atttaaaaat tgataaaagt atgcaatttc ttgttgctaa aacatttttc
     3421 aaaaaagcag gagttgatat tccacttgaa attgaagaaa aggaacattt ttatgataca
     3481 aaacaaattg caagaaagtt aggactttac acaatgtcta ataaaccagc atttatggca
     3541 gttaagcaaa ttataagaaa gcttgatatt aaagaagatg aaatgaaaac agtattagaa
     3601 acgaatggca attggactgg tactgtaaca aaatattcta ataaggttac agaaaaggtc
     3661 aaagtgtggt tgaaagaaaa taataatcct atctcaatca aaggagacaa gaaaaactac
     3721 tatatattgt ataaaaataa ttagatttta gtttagtttt gagggggatt aatacaatgt
     3781 atgagaattt acttgatatg gatagaatag aacttattag agaacttgga agtatctttg
     3841 aaaaaatgaa aaatgaaaat ccagatgaat tttatagatt tgtaagttta gtgaaagaag
     3901 aatgtaggaa aaaaagagaa agagaataaa taatatagat aaagcactta taagtacata
     3961 aaagtgcttt atcttccaaa atatgctata attgtagtat caaatatata gggtgattac
     4021 gcatcctaag tgtgagatta tcccaagcaa ttggaattga tactcacaaa gatgattact
     4081 aaataaagta attatcaccg tcccaataaa cattgggtaa aaacatctga aatttaaaaa
     4141 cacttgggat aaatttgatt aatgttagta tttacaatgg ttatgttgtt ttattttatt
     4201 taagaatgtg catttttaag tggttgaaga ataacatgag atgtttttaa atagtattag
     4261 aaatacttcg cttgtttgga aatcataagt tgaagaataa catgagatgt ttttaaattc
     4321 atctaaaagt ttagcccaag tttgaactgc tatcgttgaa gaataacatg agatgttttt
     4381 aaatctatct cttcttttac cttctataaa atttaattgt gttgaagaat aacatgagat
     4441 gtttttaaat ctattagtta ctttatagct tacatagtaa atgcctgttg aagaataaca
     4501 tgagatgttt ttaaatactg tttctctgtt cgaagtatgt gctacatctc caagttgaag
     4561 aataacatga gatgttttta aatgaaagcc acgcttgcgc attagactgt ttgaagttgg
     4621 ttgaagaata acatgagatg tttttaaatt gaataaattc ttttgtattt ttatcaaaat
     4681 acggctcgtt gaagaataac atgagatgtt tttaaatatg tgttgaagca gaagcttttg
     4741 agaatggttt agagttgaag aataacatgg gatgtaaaaa taaatattta tataaaacac
     4801 ttacttaaat agtaggtgtt ttttaattga aaggatgtga ttataatgta aaaattttga
     4861 agatatagta taatattctt ataaaatata atttaggggg atattatgag agaaggaaag
     4921 agaagaagag gatgtctatt ttggtttatt cttattattt tattttctgg agttgttgga
     4981 gcaatagcag ggaacagtac taataatgaa agcactgaaa aacagaaaga ggatttaact
     5041 aaatatattg gtgaagaagg tagtatagga gatttaaaat taactgttaa tagcatttca
     5101 aaagctagtg aaatatcagt agcatctggt tatttagcat acactccaga tagtggcaaa
     5161 tatggtatta taaatttaac aataaaaaat caaactaagg aaagccaatc ttttatgcta
     5221 aattcattca cattaatagg accagatgat tcaaaatatg ttccatcttt attgattgat
     5281 gtaggaaata aatatataac tatggatact gtaaatccta atttagatgt aacaggtaat
     5341 attgcatttg aaattcctaa ggatttacta gtttctgatt gtaaactaaa atatagtggt
     5401 tcagagcaag aatttatatt aaaagaaaaa taatatatag atttaaaaat atgaagcatt
     5461 tactttaaat aagtaagtgc ttttttaatt gaaaggaggt gataataatg taaaaatttt
     5521 acttatatag tataattaca ttataaaatt attgtactgg gggggaatct tatggggtta
     5581 tttggaaaga aaaaagcaga tatatgttgt atttgtaata ctgagatagg agtactgaat
     5641 attgaagatg gttggatatg caattcttgt tttaaagaat attgtgatgc actttctatg
     5701 actaaagcac ctaaaatttt aagaaaatta gatattgaaa agactatatc atcaactaaa
     5761 aaaaataatg aacttcaaaa aatatttaat acaactaata atatagaaaa ctatatagaa
     5821 tttgatgaag ataataaaaa atggtttgtg tctaaaaaaa gtataaacga taaaaaaact
     5881 cctatcattc attcttatga agatatcgtg gaatttgaac ttcttgaaaa tggtgaaact
     5941 gtaactaaag gaggaatagg aagagcttta gcgggaggaa ttttatttgg agaagcagga
     6001 gctatagttg gagggataac aggtaaaaaa acaacaagaa aagttgttga tacatttaaa
     6061 attaaaataa caattaataa tattgataat cctattgagt atatcgaatt aattaataaa
     6121 aaaacaaaaa ctaattctag tgcttatgag aaagcttaca aagatgccca taaaatttta
     6181 tcaatattat ctgctattac acaaagcatt aaagagacag acaatataaa tactaaatct
     6241 gtagcagatg agatattaaa atataagaat cttttggata tggaagctat tacacaagat
     6301 gaatttaata ctaagaaaaa agaattattg aatttgtaat ataataaaca cttactaatg
     6361 taggtgtttt tttatatgga aatttatgaa aggagagtga ggaaatggct acaatacaaa
     6421 cttcaataaa gattttcgac ggaatgacac cagcatttcg taatatgact aattctatta
     6481 atacaacaat taatagttta gagagattgc aaggcagatt gaataatcca ctcaatgcag
     6541 gtaatataca agcttctcaa caaagtttga ataacataga aagtattctc acaaggatag
     6601 aacagaaaat tggaagaaat acaaatgaac aagaaaactt taataataaa ataagacaag
     6661 gaagtgaagc aggctctcta ttagtatcta aactcaaaag tttggctggc atatatattg
     6721 gaataagagg tatagaaagt attacaaaag cagcagatac aattgcaagt acaaaagcac
     6781 gtttaaattt aatgaatgat ggattacaga caacagaaca acttaataag atgatttatt
     6841 tatctgctca aagtgctagg gcttcttatg cagacacagc agcacaagta tctaagttag
     6901 gaatacttgc aggagaagct tttggaagta gtgcagaagt tgtaaaattt gcagagttaa
     6961 tgaataagag tttcgtaatt ggaggaacat cagcatcaga agctagtgca gcaatgtatc
     7021 agttaacgca agctatggta gcaggaaaat tgcaaggtga tgaatttagg tctatcatgg
     7081 aaaatgcacc acttctagct actaaaatag ctgaatctat gggtaagggt aaagaagcac
     7141 taaaagattt gtctcggaca ggtgctatta cagcagatgt agtgagaaat gctttgttta
     7201 aagcaagtgc tgaaatagaa aagaaattcg aaagtatgcc aataactttt agtcaagcac
     7261 ttactatgat gaaaaatgat gcttatatga tatttggtca gacattagga aagataagtg
     7321 gagcattgca aagcgttaga tttagtgaaa ttgttgtatc tataagaaat gttatgattg
     7381 caatatcttc aaacatttac gatacattaa atattataaa aaatatttta aatagtgaat
     7441 tcttttcaaa cttagtacaa ggatttacat tttgtgtggt agtaataact aaaagcttag
     7501 gttctattgt aaatactgca ttaaatatag ttaatatatt tgcacaaaat tggagtataa
     7561 taaaacctat tgtatttggt gtagcttctg tttttatatt gtttagaggg gttttgctag
     7621 cgactaaaat agctacaata ggtaacgtaa ttgcgaatgt tgctcacgct gcttcctctt
     7681 cactatcagc acttatgact aatatacaag cagcagcact gatgcgttcg aatggagcta
     7741 ctttatcagc aacaatagcc acttggggtt taaatgcagc tttattagct tgtccaatca
     7801 catgggtagt attaggtatt ctagcatttg tagtagtagt ttttgttgca gtagcagcag
     7861 taaacaaatt cgcaggtaca agtttaactg ccttaggagt aattgtgggt gctgtatttg
     7921 cagcagtggc agcaatacaa aatgttatga tatggctact gaatggctgt atagctgtaa
     7981 atgaaggcat tacaaatggt tggaatcaat gtgtatatct tatgaaacaa gctattgcaa
     8041 aaggtgtaat ctttataatc gagaaaatgg catctctaaa tgattctgta aataatgcag
     8101 gaaacgcact tggtaaggct tttgtggctg gggcgaatat agcaatcaga ggagtaaata
     8161 agcttattga tttactaaac aaaattcctg gagtaaatat tgg
//
LOCUS       DAEPIN010000117         8178 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.119, whole genome shotgun sequence.
ACCESSION   DAEPIN010000117 DAEPIN010000000
VERSION     DAEPIN010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8178
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.119"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            48..746
                     /locus_tag="KR250_003494"
     CDS             48..746
                     /locus_tag="KR250_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069377.1"
                     /translation="MKISSQYRSQYSFRYESNINNTRINESMVKKNETVGKDTYLSNI
                     MKQKQELNDRIRDLKYRQEVYTKKINDAIKNLCKSEIRETTNNFSNIEIGIKNSIIEE
                     KNKSTMLDENSTYLNTNDEKESLITKESNEKIEEEILNDEKLEELEQKKDYKEDSNKK
                     EKVSEDLSLVGKTREELENMLKNFINLTQEEIMKLESRIEKLDKNAEEYKQNSKTNIF
                     DKTDEQKKHINVLI"
     gene            924..1814
                     /locus_tag="KR250_003495"
     CDS             924..1814
                     /locus_tag="KR250_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069378.1"
                     /translation="MKKNILTTMITMIIILMMSMTVMAEPYQEDETSSLKKQSANERN
                     QTNIGTMDGRVQTNAISKGANYDPKYPLKGMLTQLGLNTTGKEGWDLLYGGNPNGVEL
                     RADGKYYYINKGMEGLGFYAAALSGGSYQRIGFGKLDPIEVIADGNWISYKQAQDTLT
                     VIRNFLNSFDWRNASEMERANRAAKLVTEAKYADSKYCNIVYGNLVDKRGVCGSFASS
                     FHLLTRLMGMDSLSILNPSLNHAWNYIQIDGKWYRFDGSEISAFGGSLDFDYRKLKDA
                     TREMTTYYDAKALSILGFNQ"
     gene            complement(2207..3163)
                     /locus_tag="KR250_003496"
     CDS             complement(2207..3163)
                     /locus_tag="KR250_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBH0069379.1"
                     /translation="MENNTIQKQLRDLSKKIWHIYILNPEEENLKFIISLLDDNLALI
                     GTGKHEFYETLEEFIIGLEKDQTESEQIDFEIIDEWYSCTKVTDEAFIVYGTLWVIEK
                     DYLEKDIYVEMDTRFTMVYRVTDDMIKLIHVHHSIPNFDQMKDEYYPKTITEKAQEAL
                     AMANEFKLKSEKDLMTGLYNRHFFEVHIKEMLKTVSHGYFYIFDLDNFKQVNDIFGHV
                     RGDEILIHFSNILTKVFVNDAIIGRLGGDEFAVFEYSNQSKENVENKAKKVLELCLES
                     NSCSIGISKLENNSDTFQSLYKQSDYALYYSKRNNKGNFSWF"
     gene            3866..4861
                     /locus_tag="KR250_003497"
     CDS             3866..4861
                     /locus_tag="KR250_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBH0069380.1"
                     /translation="MSIIELNELTKSYRRFEKKAGFMGSVKSLFNRKYTEKTAISSFD
                     LKIEEGEFVGLIGQNGAGKTTLIKMLTGIIAPSSGEISVMGYYPNKLENNFKKQYAVV
                     MGQKSQLFFELTPADTFLLFKELYDISNEEYKKNLDYFIELFEVSDYLNVQVRTLSLG
                     ERMKMELIVALLHNPKILFLDEPTIGLDAIAQKQIRYFLKEVNETKGTTIILTSHYME
                     DIKSLCSRSIVVENSRKIYDGSTEKLFNYFQKYKKITVSFSNETNIVFPKDCKVVEQT
                     NHKISVIVPKERGNETIKWIFDKYDVKDVMIEEEDIGSIVERIYKKGSDEYYEQL"
     gene            4839..5702
                     /locus_tag="KR250_003498"
     CDS             4839..5702
                     /locus_tag="KR250_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HBH0069381.1"
                     /translation="MSIMSNYNEASSNDSMFYKFKQSIRRYYEVAKINFKVQLIWRFD
                     VVVNMVFTIVKILFAYILWKAIFGENKIVSGFTFNSMLSYYIISSFLAGIDMSKSTSE
                     EICNRVKLGTFSKYMIIPINVQNYFIAKNFGMTCFYFVFNMISAFVWIFIFKIDFAFT
                     SNPVQILISILMIFLGFIFMMKLHFFIGILSFKFLEISFLKMIIENIVEFITGTMIPL
                     VLLPETIVTVMKVFPFYYTTYLPSMLLIGRNSNEAIQGLIILALWVLFFSVVNKVMYK
                     RMRTLFDGVGI"
     gene            5699..6493
                     /locus_tag="KR250_003499"
     CDS             5699..6493
                     /locus_tag="KR250_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HBH0069382.1"
                     /translation="MMKKNLKIIFMLLKMKLSKMMVFRFDFFGAFFVDSSLFILQLLM
                     FNSIYSHVDSIGGWQQGEMLIFIGTFSLINAINMTIFFFGIYDIPRKIQEGELDYYIT
                     KPLDPLFRLTFENINIGSSPLILASILIVLYGISELNSEITLVMIIIYIFMVLLMTLL
                     FYDVCIILRTVSFFVISSSAIMRLEDNLLPMNMKIPGVIYEGIFKVLFYLILPYGIMS
                     TIPTQILAGTITINGLIYSILIVFLFTLFMWRFWKLGMRHYKSASS"
     gene            6778..8157
                     /locus_tag="KR250_003500"
     CDS             6778..8157
                     /locus_tag="KR250_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBH0069383.1"
                     /translation="MSDVYYSENHEQDNLYEQDNLLAQRFNLFTLLKFAFPTIFMMIF
                     MGLYTIVDTIFVSRFINTNALSAINIVCPIINLIVGIGTMLATGGSAIVARKMGNGDI
                     EGANKAFSLIVLSGVVIGVIITGIGIVFMDYIIIWLGASEVLIKYCRSYLFIILVFAS
                     ANILQVLFQCLFVTAGKPQFGLWLVLGAGCANIILDYVFIVLLQMGISGAALATGVGY
                     LIPTISGMVFFSKNKGLLKFCIPRLDKRILFEACFNGSSEMVGQLSTAVTTFLFNAMM
                     MKLIGEDGVAAITIIIYSQFLLTSLYIGFSMGVAPIISYNYGSENNTQLNHVFKICIL
                     FISVASLLVFIFSISCSPIIVEIFSQRGSRVYEIARSGFYIFSFSFIFCGYNIFTSSM
                     FTALSNGKVSAILSFLRTFGFITIGLLILPNILNINGIWLAVPSAEILTLFLTVFCIF
                     KYKNRYKYM"
BASE COUNT         3032 a          876 c         1287 g         2983 t
ORIGIN      
        1 taagttggtt tttataaaca taaatttatt ttaaggaggt gaaataaatg aaaatatcta
       61 gtcaatatag aagtcaatat tcatttagat atgaaagtaa tataaataat acaagaataa
      121 atgaaagtat ggttaagaaa aatgaaactg taggaaaaga cacttattta tctaatatta
      181 tgaaacaaaa gcaagaactt aatgatagaa ttagagattt aaaatataga caagaggttt
      241 atactaaaaa aataaatgac gcaattaaga acttatgtaa atcagaaata agagaaacaa
      301 ctaataattt ttctaatata gaaataggta ttaaaaatag cattatagaa gagaaaaata
      361 aaagtacaat gttagatgaa aattcaactt atctaaatac aaatgatgaa aaagaatctt
      421 taatcactaa agagtctaat gaaaaaattg aagaagaaat attaaatgat gaaaaattag
      481 aagagttaga acaaaaaaag gattataaag aggattctaa taaaaaagag aaagtatctg
      541 aggacttatc tttagtaggt aaaactcgtg aagagcttga aaatatgctt aaaaatttta
      601 taaatttaac acaagaagaa ataatgaaac ttgagtcgag aatagaaaag ttagataaaa
      661 atgctgaaga atacaaacaa aattcaaaga ctaatatatt tgataaaaca gatgaacaaa
      721 aaaaacatat aaatgtactg atttaaacaa tatgttttaa aaaaataagt ttattatata
      781 atgaaaatat aaaggtacaa tttcaataca cttctattaa accaataatt tatgaatcag
      841 atttaatttc aatttactaa taaccatata tttatgagtt aataatttta tgttttaaaa
      901 agaattaaat aagggggagt agtatgaaga agaatatatt aactactatg attaccatga
      961 ttattatatt gatgatgagt atgacagtaa tggcagagcc atatcaagaa gatgaaacat
     1021 ctagcttgaa aaaacagagt gcaaatgaac gtaatcagac taatataggc acaatggatg
     1081 gcagagtgca aacaaatgca ataagtaaag gtgctaatta tgaccctaaa tatcctctta
     1141 aaggaatgtt aacacaactt ggtcttaata caacaggtaa agagggatgg gatttactct
     1201 atggaggaaa tcccaatgga gttgagcttc gagcagatgg taaatattat tatataaata
     1261 agggtatgga agggctagga ttttatgcgg cagcattgtc aggtggctca tatcagagaa
     1321 taggctttgg aaaacttgac cctatagaag taatagctga tggaaattgg atttcttaca
     1381 aacaagctca agatacactt acagtaatca ggaatttttt aaatagcttt gactggagaa
     1441 atgcatctga gatggaaaga gctaatagag ctgcaaaatt ggtaaccgaa gcaaaatatg
     1501 cagactctaa atattgtaac atagtctatg gtaatctggt tgataaacgt ggtgtatgtg
     1561 gaagttttgc aagtagcttt catttattaa caaggttaat gggaatggat tcactatcta
     1621 tattaaatcc atcattaaat catgcttgga actatataca aattgatggg aaatggtata
     1681 ggtttgatgg gtctgaaata tcagcttttg gtggatcatt agactttgat taccgtaaat
     1741 taaaggatgc aacaagagag atgactactt actatgatgc taaggcattg tctatattag
     1801 gatttaatca ataaaaatag ggggaggctt cttatagagc ctttttttat tgatttgaaa
     1861 tttaataagg aattttatat gaaaatttaa tcaataaaag caaaaaaggc tctttataaa
     1921 gcctttctaa gtgttttttt aagtaaaatt ttttatatca acatcagttt taatcaaaac
     1981 atataacatt ttggacttac ttttaaaaat aaaattatta aaatataata ttaatcatgt
     2041 ttttagttta ttatattcta taatagtggt catgttgact attgtagttt tattaagaat
     2101 agttggttta tataaatata tgcaaagata gcttgttgtt tactttactt attagtatat
     2161 aggacaagat actctaataa atttgcctat tttaatatct tttgtactaa aaccaactaa
     2221 agtttccttt attatttcgt tttgaataat acaaagcata atccgattgt ttgtataaag
     2281 actggaaagt atcagagtta ttttctaatt tagatatacc tatagaacaa gaattactct
     2341 ctaggcataa ttctaaaacc ttctttgcct tattttcaac attctctttt gattgattac
     2401 tatattcaaa aacagcaaat tcatctccac caagtcttcc aataatagca tcatttacga
     2461 atactttagt caaaatattt gaaaaatgta ttagtatttc atctcctcga acatgaccaa
     2521 agatatcatt tacctgttta aaattatcta aatcaaagat gtaaaagtat ccatgtgaaa
     2581 cagttttaag catttctttt atgtgtactt caaaaaaatg tctattgtat agtccagtca
     2641 taaggtcttt ttctgatttt agtttaaatt catttgccat tgctagtgct tcttgtgctt
     2701 tttcagttat tgttttaggg taatactcat ctttcatttg gtcaaagttt ggaatagaat
     2761 ggtgaacatg gataagttta atcatatcat ctgtaactct gtataccata gtaaatcgag
     2821 tatccatttc tacatagata tctttttcta ggtaatcttt ttctattacc caaagagtac
     2881 catatactat aaaagcttcg tctgtaactt tggtacatga ataccactca tctataatct
     2941 caaagtctat ttgctctgat tctgtttggt ctttttcaag acctataata aattcttcta
     3001 atgtttcgta aaattcatgt tttcctgttc caattaatgc aaggttgtca tctaataagg
     3061 aaataataaa tttaaggttt tcttcttctg gatttaaaat ataaatatgc caaatttttt
     3121 ttgataaatc tcttaattgt ttttgtattg tattattttc catagattac ccccttaaga
     3181 tttacagttt atattttaat gtattataag ttgaactctt attacaatat tattataata
     3241 tagaactaaa gaaattacat cctgtataag taaaagtata tcttttataa taaaaatatt
     3301 acagtaaaat aaccagtttt aatatacgta ttatattttt aatttataaa cgaatattat
     3361 aaatgaaatt taaattcaac cctgaagaaa taagctaaat gtattagtat ataaatccat
     3421 acttgaacat aaataacctt tatgatatga tatcaataat aatattatga gaggagtgcc
     3481 ttgatgtcgg agttatataa tggtaaccaa caattaaata gttggagaca catatattct
     3541 gtataatcag aaatttcaag tgtattttta atacatttaa tttttgtgtc ttattctgcc
     3601 tttatagata actgacatat gacaacaaat agtatttaaa atatatatta ctttgttaaa
     3661 ttatggtgta gtaggcttta atctgctaca cttttattaa ttttgtatag tattattttg
     3721 ataactattt tgaaatagag agcattaatt tttctagtcc acaggagttt tctgtttttg
     3781 tgctgttaaa tataagcaca gagaaaggca gatatatacc tgtggacttt ttttatataa
     3841 attatgaaat atggaggtag ttaaaatgag tataattgaa cttaatgaac tgactaaatc
     3901 ttatagaaga tttgagaaaa aggcaggatt tatggggagt gtaaagtctt tatttaatag
     3961 gaaatataca gaaaaaacag ctatatcgtc atttgattta aagatagaag aaggagaatt
     4021 tgttggatta ataggtcaaa atggagctgg caaaactact cttattaaga tgttaactgg
     4081 tattattgca ccgtcttctg gagaaatatc tgtaatgggt tattacccta acaaattaga
     4141 aaataatttt aaaaaacaat atgctgttgt tatgggccaa aaaagtcaac tattctttga
     4201 attgacacca gcagatacgt ttcttttatt taaagaatta tatgatatat ctaatgaaga
     4261 atataagaaa aacttggatt actttatcga attatttgag gtatctgact atttaaatgt
     4321 tcaagttagg acgctttctt taggagaaag aatgaaaatg gaactaatag tagcccttct
     4381 acataatcca aaaatattat ttttggatga accaactata ggcttagatg ctatagccca
     4441 aaaacaaatt aggtatttcc taaaagaagt aaatgaaaca aaaggaacta ctattatact
     4501 tacttctcat tacatggaag atattaagag tctctgtagt agaagtattg ttgtagaaaa
     4561 tagccgtaaa atatatgatg gttctacaga gaaactattt aattattttc aaaaatataa
     4621 aaaaataaca gttagttttt ccaatgaaac taacattgta tttccaaaag attgtaaagt
     4681 tgttgaacaa acaaatcata agatatcagt aattgtgcca aaagaaagag gaaatgaaac
     4741 gattaaatgg atttttgata aatatgatgt taaagatgta atgattgaag aagaagatat
     4801 aggtagtatt gtagagagga tatataagaa aggaagtgat gagtattatg agcaactata
     4861 atgaagcaag ttcaaatgat tcaatgtttt ataaatttaa acagagtatt agaaggtatt
     4921 atgaagtagc caaaattaac tttaaagtgc aacttatctg gagatttgat gtggtggtta
     4981 atatggtgtt tacgattgta aaaattttat ttgcttatat tttatggaaa gcaatttttg
     5041 gtgaaaataa aatagtctct ggttttacat ttaactctat gttatcttac tatataataa
     5101 gctcattttt agcaggtatt gatatgtcaa agagtaccag tgaagagata tgtaatcgtg
     5161 taaaattggg caccttttca aagtacatga ttattccaat taatgttcaa aattatttta
     5221 ttgctaaaaa ttttggaatg acatgttttt attttgtatt taatatgatt tctgcatttg
     5281 tatggatttt tatatttaaa atagattttg cctttacatc aaatccagtt caaatattaa
     5341 tttcaatttt gatgattttt ctagggttta tttttatgat gaaactccac ttctttattg
     5401 ggattttgtc atttaaattt cttgagatta gttttttaaa aatgataatt gaaaatattg
     5461 ttgaatttat tacaggaaca atgataccac ttgtactctt accagaaact atagtaacag
     5521 ttatgaaggt atttccattt tattatacaa catatttgcc atctatgtta ctaataggta
     5581 gaaacagcaa tgaggcaata caaggattaa ttattctagc attatgggtt ttattttttt
     5641 ctgtagtaaa taaagttatg tataaaagaa tgagaacatt atttgatggg gtgggtatat
     5701 gatgaaaaaa aatttgaaaa taatatttat gttattaaaa atgaaattat caaagatgat
     5761 ggtatttaga ttcgattttt ttggagcatt ttttgtagat agttcattat ttatattaca
     5821 acttctaatg tttaatagta tatattctca tgtagactca atagggggat ggcaacaagg
     5881 agagatgtta atatttatag gaactttctc attaataaat gcgataaata tgacgatttt
     5941 cttttttggt atttatgata ttccacgaaa aatccaagaa ggagaattag actactatat
     6001 tacaaaacca ttagacccac tatttaggtt aacatttgaa aatattaata ttggttcatc
     6061 accattaatt ctagcaagta tacttattgt tttatatgga atatctgagt taaactcaga
     6121 gataacttta gttatgataa taatatatat tttcatggtg ttattgatga cattactttt
     6181 ttatgatgta tgtatcatac ttagaacagt ttcatttttt gttatatcat cttctgcgat
     6241 aatgagatta gaagataatt tattgccaat gaatatgaaa attcctggtg taatatatga
     6301 aggtattttt aaagtattat tctatttaat cttaccatat ggaattatgt cgacaattcc
     6361 gactcaaatt ttggctggga caataacaat taatggatta atatactcaa ttttaattgt
     6421 atttttgttt acattgttta tgtggagatt ttggaagtta ggtatgagac attataaaag
     6481 tgcaagtagt taataaatta gtaaaaatag acaatgtata actatatata aatacgtttc
     6541 atatgacact aaactatgtg aacaagtatt tgcgctaaag gcactgaaag ctataaaatg
     6601 attaaagtaa ggatttaaaa gtttctttag tagaaatata attgtgtaaa aaagcaagat
     6661 taattaaaac tatatccaaa taattagata taattcttat gcagaaagga ggtctgtaaa
     6721 tgtctaattg tttggaatat attttaaaaa taattaatgt acagaaaaat aaacaaaatg
     6781 agtgatgtat attattcaga aaatcatgaa caagataatc tttatgagca agataattta
     6841 ctggcacaaa gatttaattt atttactcta ttgaagtttg catttcctac tatttttatg
     6901 atgattttta tggggttgta tacaatcgta gatactatat ttgtatcaag atttattaat
     6961 acaaatgctc tatcagcaat taatattgta tgtccaatta ttaatcttat agttggaatt
     7021 ggaactatgc ttgccactgg aggaagtgct attgtagcac gtaagatggg aaatggtgat
     7081 attgagggag caaataaagc tttttcttta atcgtattat caggagttgt aattggggta
     7141 ataattactg gtataggtat tgtatttatg gattatatca ttatatggtt aggagcaagt
     7201 gaagttttaa ttaaatattg tcgttcatat ctttttatta tcttggtttt tgcttctgca
     7261 aatatactac aagtattatt tcaatgttta tttgtaacag ctggaaagcc tcaatttgga
     7321 ttatggctag tgttaggtgc tgggtgtgct aatataattc ttgactatgt gttcattgtt
     7381 ctgttacaaa tgggtattag tggagcagca cttgcaactg gagtaggata cctaattcct
     7441 accatttcag gtatggtttt cttttcaaaa aataaagggt tacttaaatt ttgtatacct
     7501 agactagata aaagaatatt atttgaagct tgttttaatg gttcatctga aatggtgggt
     7561 caactatcaa cagcagttac tactttttta tttaatgcta tgatgatgaa attaattggt
     7621 gaagatgggg ttgcagctat tacaataatt atttattctc aatttctttt aacttcgctt
     7681 tatataggtt tctcaatggg tgttgcacca ataataagtt ataactatgg aagtgaaaat
     7741 aatacgcagt taaatcatgt ttttaagatt tgtatattat ttatttcagt agcatcttta
     7801 ttggtattta tcttttctat atcatgttca ccaatcattg tagagatttt ttctcaaaga
     7861 ggaagccgag tttatgaaat tgcaaggtcc ggattttata tattttcttt tagttttatt
     7921 ttttgtggat ataatatttt tacctcatct atgtttacag cattatcaaa tggaaaggtg
     7981 tctgcaatat tatcttttct acgtacattt ggatttataa ctataggttt attgatattg
     8041 ccaaatatat taaatataaa tgggatatgg ttagcagtac caagtgctga gattttaacc
     8101 ttatttttaa ctgttttttg tatatttaag tataagaata gatataaata tatgtagata
     8161 ttaaataaat agcttaga
//
LOCUS       DAEPIN010000118         7925 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.120, whole genome shotgun sequence.
ACCESSION   DAEPIN010000118 DAEPIN010000000
VERSION     DAEPIN010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7925
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.120"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            9..488
                     /locus_tag="KR250_003501"
     CDS             9..488
                     /locus_tag="KR250_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HBH0069384.1"
                     /translation="MSNKLIKNLLSGILQILFFLLGLVIVVGGFKSFMYLCFSGEATL
                     QGTIYGILMFILGVSYFIIIKSLIEVLGSSEYSLFVKENVKRFRIIGYLLLLNSLIEF
                     ISTFGTTGKGMRFLDLGFGFYFTVPVFVYFITSLMSFVIADGFVKAIKIKEDNDLTI"
     gene            1126..1503
                     /locus_tag="KR250_003502"
     CDS             1126..1503
                     /locus_tag="KR250_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBH0069385.1"
                     /translation="MMIRKIPQAELKVMKFIWKVDVTVTSKDVIEAMEQKYGWKQTTT
                     LTLLSRLVKRGFLDAQKIDRYTHYTIRVKHKDYLSFETKDFLSNIHDDSLRSLISALH
                     DDENIGKDKLDFLESYFDNLKDE"
     gene            1496..2644
                     /locus_tag="KR250_003503"
     CDS             1496..2644
                     /locus_tag="KR250_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease"
                     /protein_id="HBH0069386.1"
                     /translation="MNNYMLYVFENLIKTTIVCSLGICLLLFLKRYLFKKFSKRFNYY
                     IWLIIVFRMLLFLFNYTIVYEVKEPKENALGNNITQIDISTDNNLMLYVAYLWLFVTI
                     VIAVYTFIKYTRFKNLVVDVSYDIEDNDINCIYKNLLKELNIKKKIELRGSDELISPA
                     GMGLFKSYIFLPDYPYSKDELTWILKHELMHFKNKDILIKFLVLSVRIIYWFNPLVYV
                     MSNKVNLDCELCCDESVLVECSLKDKKEYALALIKSIKFSKNYNSKILTTEFDKTNLE
                     KRLESIVKKKGKSGILIAVLVFMTSITSFVEVDAQVRIDEGNNGMNNNINEFLLNKTT
                     DKIINIYYKDAPNEIRKFYEDKCELEGKIPRDYDIIEMDMKDYNQLIK"
     gene            2661..3263
                     /locus_tag="KR250_003504"
     CDS             2661..3263
                     /locus_tag="KR250_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HBH0069387.1"
                     /translation="MLTKLRKIVAIMCCLLLTFTSVAFADTSKEKGGSKETVPEKNLD
                     SFTLSGYIYTYENAPQEIRDEHKQNCEEINIDPKPDDEIFVPESANLMNEDNSKGVYA
                     SYTVSYNIGAKTITIMSNEYLTISTTKVIRKGNSGKEVKAAQIMLTLLGYNVGIDSSF
                     GSKTYNAVVSFQKKYGLSADGIIGPATWDKLGRLTDPTLS"
     gene            complement(3356..4378)
                     /locus_tag="KR250_003505"
     CDS             complement(3356..4378)
                     /locus_tag="KR250_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBH0069388.1"
                     /translation="MDYNDLLKSARENFNGTCKVCKICNGLACAGDVPGMGGKGSGSS
                     FIENRKSLEKIKINMRVIHNVSKPDTSIELFGRKMSSPIFAAPVSGTILNMGGKVSEK
                     EYIEPVVRGCSNSGIYAMVGDTNVDTFLLDNLDVLKDNRGNGIVFIKPWNNSKIIDKI
                     RLSEEAGAFAVGVDLDACGLINNQFQENPFSPKTIDEIRELVESTRLPFIIKGIMTVD
                     DALMAVESGASAIIVSNHGGRVLDYTPGTCEVLPDIAKSVKGKITILVDGGVRTGVDV
                     VKMLGLGADAVLMGRPFVTASFGGGLDGVEFFIEKVRNELCETMILTGCQNVKDIDGR
                     VIWNLL"
     gene            complement(4707..4889)
                     /locus_tag="KR250_003506"
     CDS             complement(4707..4889)
                     /locus_tag="KR250_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069389.1"
                     /translation="MYKDKSDECIHLMTAYIDSISGYYSFIDTQLEDFMMKYGENIVD
                     SNLHSIMMLLCKWGLS"
     gene            5202..5621
                     /locus_tag="KR250_003507"
     CDS             5202..5621
                     /locus_tag="KR250_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3785 domain-containing protein"
                     /protein_id="HBH0069390.1"
                     /translation="MSLYKFIYDDKEYLLKEENCSALINDEENPVQGVSISKIIDILN
                     EAEEVDFDVEYYQEACPLCLEGVKEKKKFFPFLEYHFYIFSKDGEYVISNISVDYKGL
                     SFNKLSRANKVDNSYIVSVIICENCQDYIVQIENCIV"
     gene            5752..7593
                     /locus_tag="KR250_003508"
     CDS             5752..7593
                     /locus_tag="KR250_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBH0069391.1"
                     /translation="MEEIDDTILDSMFKLTLNERLLTNNDFIENKIDVVKNLIDCYKT
                     EQNSLMLLKSNFLLALLYGIQGFSEKMKEYILISCKYIDKCLPKDYKFLARFYSQIAI
                     LSLKNEDVNKANLYMDKFNLICDENNFLIEEIIFKSQLIYIKASKCIESSVIIKEIET
                     LYIKIKEINDFNCKKIYFFIIGKIYFLFLDDALIAKVNFLKARKYADLLKDMQVSSLC
                     NIKIGECECSFENYECSMEYFNEVISNKKYRNVNIIQKYRASNNITKILIKTRNYPVA
                     INNLAKSEVYLEKIKNHSLKEVEKFDLFISLAMYYAKSNEKSFEQSICYLDRAKNILN
                     MIKNIEEYIIYDLEIIYHQAKIYYIFGYYDNALNTNKKLLEKSKNAKNYNYVKLAYKS
                     IYLCFEKIQNYEMSMKYFKMYYELKMMYIKARNRNYIDSLNYRHEYIEKEINNMRKVK
                     LDLDKKKYVDHLTSAYNRTFLNDFLEKQEVSEYCTAFMIDVDYFKEYNDTYGHYNGDI
                     ALKNITIIIKKYLKKDMLMIRYGGEEFLILSVCKDYKKSKIFGRKLCRIVKKFLNNNL
                     TVSIGIDTCKNSSIDIKEIIENADKALYKAKESGRNRCLHYHDFKNF"
     gene            7620..>7925
                     /locus_tag="KR250_003509"
     CDS             7620..>7925
                     /locus_tag="KR250_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBH0069392.1"
                     /translation="MKRSLIEDLKRNINKIEYDMLTKFYLNLTDLNYILDYHKDLLNT
                     KLEDNSRVYTLYLISLINYIKKDREASYNYLIESLKYIKKLSLKDDSDIISKIYIYLT
                     "
BASE COUNT         3217 a          824 c         1128 g         2756 t
ORIGIN      
        1 ttggcatgat gtctaataaa cttataaaaa atttactaag tggtatatta caaattttat
       61 tttttctttt aggattagta attgttgtag gaggatttaa aagttttatg tacctctgtt
      121 ttagtggaga agcaactcta caaggtacta tttatggaat attaatgttt attttaggtg
      181 tatcatattt tatcattata aaatcattga tagaagtatt aggtagttct gaatatagcc
      241 tatttgtaaa agagaatgta aaaagattta ggattattgg atatcttttg cttttaaact
      301 ctctaattga gtttatttca acttttggta ctactggaaa aggaatgaga tttctagatt
      361 taggatttgg attttatttt actgttccag tatttgtata ttttataact tctctgatga
      421 gttttgtaat tgcagatgga tttgtaaagg caataaagat aaaagaggat aatgatttaa
      481 ctatttaggt gtgacacttt aatatatagg agataaataa cccgaaaaga gtgactaact
      541 agtaatttaa aataaaatat attagggtat aatacataaa ctataatgct aaaattaaaa
      601 gattataata ctaaaatata attatgtttt aattaaaata aagaacttta ttagtagata
      661 ttaaaagcac atttttttat aaaagtactt ttttataatt tatgaaaggt ataattataa
      721 aagtcttatt caataaaatt tataattaat ttagatatat ttgtaaataa tcagtataaa
      781 atagatgttt taatatcata aaggcataat tagaaggtta ttaaaattta ttagatatta
      841 ctctagttta ttttaaaaaa aagtattata atattccttg tgtgaaaata taggaatttt
      901 tattaaatag ttttaatgtg taaataacat tctacactat tgtaattaaa tatgtaaaaa
      961 gtaaaatatt taaatagata tattttaaaa tctaactttt aatattgtaa aaaaattaga
     1021 aatttaacaa aaagtacaaa aattcacatt tttttcataa agtgtttgac taaaaaattc
     1081 acatgttgta gaattaaggt agcaggagtt ttgagggagg tgtaaataat gataagaaaa
     1141 ataccacaag cagaactaaa agtaatgaaa ttcatttgga aagtagatgt tacagtgaca
     1201 tcaaaagatg ttattgaagc tatggaacaa aaatatggct ggaaacaaac caccacactg
     1261 acacttttat ctaggctggt aaaaagagga ttcttagatg ctcaaaaaat agacagatat
     1321 acacattaca caataagagt aaaacacaaa gattatttaa gttttgagac aaaggatttc
     1381 ctaagtaata tacatgatga ctctttaaga agcttaattt cagcattaca tgatgatgaa
     1441 aatataggta aagataagct agattttcta gaaagttatt ttgataattt aaaggatgaa
     1501 taattatatg ctatatgttt ttgaaaatct tataaagaca actattgtat gtagtcttgg
     1561 tatttgtcta ttattatttt tgaaaagata tttatttaag aagtttagta agagattcaa
     1621 ttattatatt tggttgataa tagtctttag aatgttactt tttctattta attatacaat
     1681 agtgtatgaa gtaaaagaac ccaaagaaaa tgcattagga aataatatta cacaaattga
     1741 tatttctaca gataataatc ttatgctgta tgtagcttat ttatggttat ttgtaactat
     1801 agtcattgca gtttatacat ttataaagta tacaagattt aaaaatctag ttgttgatgt
     1861 atcttatgat attgaagata atgatataaa ctgtatctac aaaaacttgt taaaagaatt
     1921 aaatataaaa aagaaaattg aattaagagg ttctgatgaa ttgataagtc cagcaggtat
     1981 gggattattt aaatcttaca tattcttacc agactatcct tatagtaaag atgaactaac
     2041 ttggattttg aaacatgaat taatgcattt taaaaataaa gatattttaa taaagttttt
     2101 agttttgtct gttagaataa tatattggtt taatccactt gtatatgtaa tgagcaataa
     2161 agtaaatcta gattgtgaat tatgttgtga tgaaagtgtt ttggtagaat gttctttaaa
     2221 agataaaaaa gaatatgcac tagcacttat aaagtcaatt aagtttagta aaaattataa
     2281 tagtaagatt ttgacaaccg agtttgataa gacaaatttg gaaaagagac ttgagagtat
     2341 tgttaaaaag aaaggcaaaa gtggaatact aattgctgtt ttagtattta tgacttctat
     2401 aacatctttt gttgaagttg atgctcaagt tagaattgat gagggtaata atggaatgaa
     2461 taacaatatt aatgaatttt tattgaataa gacgactgat aaaattataa atatttatta
     2521 taaagatgca ccaaatgaaa ttaggaaatt ttatgaagat aaatgcgaat tagaaggtaa
     2581 aatacctaga gactatgaca taatagaaat ggatatgaaa gattataatc aattaataaa
     2641 ataattataa ggaggatttt atgttaacaa aattaagaaa aatagtagct attatgtgtt
     2701 gcttattgct tacttttaca agtgtggcat ttgctgacac aagtaaagag aaaggaggaa
     2761 gtaaagagac tgttccagag aaaaacctag attcttttac tctttctggg tatatatata
     2821 catatgaaaa tgcgccacaa gaaataagag atgaacataa acaaaattgt gaagagataa
     2881 atatagaccc taagccagat gatgagatat ttgttccaga atcagctaat ctaatgaatg
     2941 aggacaatag taaaggtgtt tatgcaagtt atactgtttc ttataatata ggtgctaaaa
     3001 ctataacaat tatgagtaat gagtatttga ctataagtac aacaaaagtt atacgaaagg
     3061 gtaatagcgg aaaagaagta aaagctgcac aaatcatgtt aactttactt ggttataatg
     3121 taggtataga tagttctttt ggaagtaaga catataatgc agttgtaagt ttccaaaaaa
     3181 aatacggttt gtctgcagat ggaataattg gtccagctac ttgggataaa ttgggtagac
     3241 taactgaccc tactctttca taggattaaa atctaaaaat atattaaata gtgtctcaat
     3301 atgatttttc aatttaaatg agacactatt taaatataaa tgttataaaa ctattttaca
     3361 ataaattcca tataactcta ccatctatat ccttaacatt ttgacaacca gtcaaaatca
     3421 tagtctcaca taactcattc ctaaccttct ctataaaaaa ctccacacca tcaagaccac
     3481 cgccaaaaga agcagtaaca aaaggtcttc ccatcaaaac agcatcagca cccaatccaa
     3541 gcatcttaac tacatcaaca ccagtcctaa ctccaccatc aacaagtatt gttatctttc
     3601 cctttacaga cttagctata tctggaagta cctcacaagt accaggagta taatcaagta
     3661 ctcttccacc atgatttgaa acaataatgg cactagcacc agactctaca gccatcaaag
     3721 catcatctac agtcataatt ccttttataa taaaaggtaa tctagtggat tcaaccaatt
     3781 ctctaatctc atcaattgtt ttaggagaaa aaggattttc ttgaaattga ttattaataa
     3841 gtccacaagc atcaaggtct acaccaactg caaaagcacc tgcttcttca gacaatctaa
     3901 ttttatctat tatctttgaa ttattccaag gctttataaa tacaattcca tttcccctat
     3961 tatccttcaa tacatctaaa ttatccagta aaaaagtatc tacattagta tcgccaacca
     4021 tagcataaat tccagaatta gaacaacctc taacaacagg ctctatgtat tctttttcgg
     4081 aaactttacc acccatgttc aatatagtac ctgatacagg tgcagcgaat ataggagaac
     4141 tcatttttct accaaataat tctatggatg tatctggctt tgaaacatta tgtatgactc
     4201 tcatatttat ctttattttt tctagacttt ttctattttc aatgaaagac gaaccacttc
     4261 ctttaccacc cataccagga acatctccag cacaagctaa tccattacaa attttacata
     4321 ctttacatgt tccattaaag ttctctctag ctgattttag taagtcattg taatccatac
     4381 aaaatctctc ctttttataa tattaaaatt gagtattact atattgaaaa tatcttaatt
     4441 gtaaatatag ggatgttatt atgatgagct aaactagata atacatatta gatatttttt
     4501 attgttgtag atagttaaat tatatcatat tagatagtca aattttgaaa aaaactaact
     4561 tatttaagta tacattttga aaaaattaaa aaagctatat atagattttt aaagaaatct
     4621 atatatagct tttaagttta tgtcaatctt ttttgttaat gaataaataa tatagaatac
     4681 aatatacaaa aaagtttctt gaaatgctaa gataatcccc atttacaaag caacatcatt
     4741 atactgtgta aatttgaatc aacaatgttt tcaccatatt tcatcataaa atcctctaat
     4801 tgtgtgtcaa taaatgaata atatccactt atactatcga tgtatgcagt cattaagtgt
     4861 atacattcat cacttttatc tttatacata tatatctccc cctatataca atatataaat
     4921 ttagatacca aatcgtatca tattaaatag ttaagtgtgt aacatctatg cagttatata
     4981 tttatatcat tatattaata actaaagtga gttttgacaa tagattttgg gaaactggat
     5041 gttcaaagtg caaacttgga ttttaaaatc attaattaag atagcgcata ggaatatatg
     5101 tatacataac ttaattataa agattataaa caattacttt tattaacttt atttaaatgg
     5161 tagaataact tggagattaa taattataag gaggatataa tatgagttta tataagttta
     5221 tatatgatga taaggaatat ttattaaaag aggagaactg tagtgcttta ataaatgatg
     5281 aagaaaatcc agttcaagga gtaagtattt caaaaataat tgatatactt aatgaagcag
     5341 aagaagttga ttttgatgta gaatattatc aagaagcatg tccattatgt ttagaaggag
     5401 taaaagaaaa gaaaaaattc tttccttttt tagaatatca cttttatatt ttcagtaaag
     5461 atggagaata tgtaataagc aatatatctg ttgattataa aggactatct tttaataaac
     5521 tatcaagggc aaataaagtt gataatagtt atattgtaag tgtgataatt tgcgaaaatt
     5581 gtcaggatta tatagttcag atagaaaatt gtatagttta gtgtaaagta tgtcttaatt
     5641 taagagaaat ttctaaattg agacatcaac ttttttcaaa aatagtatgg aaaattacta
     5701 aatttatgag ataatgtaag tcggaggtga gttacaatat atagtaaata tttggaagag
     5761 atagatgata caattttaga ttcaatgttt aaactaacct tgaatgaaag attattgact
     5821 aataacgatt ttattgaaaa taaaattgat gtagtaaaga atttaattga ttgctataaa
     5881 actgaacaaa atagtttaat gttattaaaa tcaaattttt tgctagcttt attatatggt
     5941 attcaagggt ttagtgaaaa aatgaaagag tatattttga tttcctgtaa atatattgat
     6001 aaatgcttac ccaaagatta taaattttta gcacgatttt attcacaaat agctatttta
     6061 agtttaaaaa atgaagatgt aaataaggca aatttatata tggataaatt taatcttatt
     6121 tgtgatgaaa ataattttct aattgaagaa attattttta aatcacaact tatttacatt
     6181 aaggcaagta agtgtataga atcttctgta ataattaaag agatagaaac gttgtatata
     6241 aagataaaag aaattaacga ttttaattgt aaaaaaatat atttttttat tattggtaaa
     6301 atatattttt tgtttttgga tgatgctcta attgctaagg taaatttttt aaaagcaaga
     6361 aaatatgcag atttactaaa ggatatgcaa gtatcttcac tatgtaatat aaagataggt
     6421 gagtgtgaat gttcgtttga aaattatgaa tgttccatgg aatatttcaa tgaagtaata
     6481 agcaataaaa agtatagaaa tgtaaatata attcaaaaat atagagcatc aaacaacata
     6541 actaaaatat taataaagac tagaaattat cctgtcgcaa taaataattt agcaaaatct
     6601 gaagtatatc tagaaaaaat taaaaatcat agcttaaaag aagtagagaa gtttgattta
     6661 tttataagtt tagccatgta ttatgcaaaa agtaatgaaa aatcttttga acaatcaata
     6721 tgctatttag atagagctaa aaacatatta aatatgataa aaaatataga agaatatata
     6781 atatatgatt tagaaataat ttatcaccag gcaaagatat attatatctt tggatattat
     6841 gataatgcct taaacacaaa taaaaaactc cttgaaaaat ctaaaaatgc taaaaattat
     6901 aattatgtga agttagcata caagagtata tatttgtgtt ttgaaaagat tcaaaattat
     6961 gaaatgagta tgaaatactt taaaatgtat tatgagttaa aaatgatgta tataaaagct
     7021 cgaaatagaa attatataga ttccttaaat tatagacatg agtatataga aaaagaaata
     7081 aataacatga gaaaagtaaa acttgattta gataaaaaaa agtatgttga ccatttgaca
     7141 agtgcatata atagaacttt tttgaacgat tttttagaaa aacaagaagt tagtgaatat
     7201 tgcacagcat ttatgataga tgtagattac tttaaagaat acaatgatac ttatggacac
     7261 tataatggag atattgctct taaaaatata actataataa taaaaaaata tctaaaaaaa
     7321 gacatgctta tgataaggta tggtggagaa gaatttctga tattatctgt atgtaaagat
     7381 tataaaaaaa gtaaaatatt tggaagaaaa ttatgtagga ttgtaaagaa atttttaaac
     7441 aataatttga cagttagtat tggcattgat acatgtaaaa atagtagtat agatataaaa
     7501 gaaataattg aaaatgctga taaagcttta tataaagcaa aggaaagtgg tagaaataga
     7561 tgtttgcatt atcatgattt taagaatttt taactgttga aataggattg gtgatttact
     7621 tgaaaaggag tttaatagaa gatttaaaga gaaatataaa caagatagaa tacgatatgt
     7681 tgactaaatt ctatctaaat ttgacagatt taaactacat cttggattat cacaaagacc
     7741 ttttaaatac aaaattagaa gataatagta gagtctatac tttatattta attagtctta
     7801 taaactatat aaaaaaagat agagaagcat catacaacta tttgatagag tcattaaagt
     7861 atattaaaaa attgtcatta aaagatgatt cagatataat atctaaaatt tatatttatc
     7921 ttact
//
LOCUS       DAEPIN010000119         7782 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.121, whole genome shotgun sequence.
ACCESSION   DAEPIN010000119 DAEPIN010000000
VERSION     DAEPIN010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7782
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.121"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            224..2107
                     /locus_tag="KR250_003510"
     CDS             224..2107
                     /locus_tag="KR250_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HBH0069393.1"
                     /translation="MSNKNKENITLSNQIKGTIQNSLTKEAILHIPMSNYAYGYDRET
                     LHIRLRTKKNEAKRVILRIGDQYVWDKGGAGGGNLNASGVGWSGGTNIVMSKEVETEL
                     FDYWIAKCKPLNKRSRYGFIIEGQEEKLLFTEKRIIELGNENDEKELCEIGNFFGYPY
                     LNYIDIPKVPNWVKDTVWYQIFPDRFANGDPSINPEGVDKWGAIPTRDNFTGGDLQGV
                     IEHLDYLSDLGINGIYFCPITIGKTNHRYDTVDYMEVDPTLGDKETLKKLIEEAHKRN
                     IKIMLDAVFNHIGYYSKQWQDVVQNKENSRYKNWFYIKDMSKIDTPIEQIDEKNIPYE
                     TFGCEKYMPKLNTENSEVIKYLLDVGKYWIQEFDIDAWRLDVSNEVDHVFWRKFREEV
                     KKVKPDIYILGEIWHGSLPWLMGDQFDSVMNYLMSEAMKKFFCTDEINAEEFKYMIND
                     VMVSYPIQVSEVIFNLLGSHDTTRILTYANGNIDRFKLAYMFMFVQTGCPCIYYGDEI
                     GMEGELSSISEGQRKCMEWNEEKWNKDIFDFMKKIIRLRKENKELRSISNEWVLADKE
                     NGTIILKKESISIIINNSSKNIILTLPDYLANKKVKDLYEEEIVDLKEELMLEKYKFI
                     ILK"
     gene            2332..3012
                     /gene="pgmB"
                     /locus_tag="KR250_003511"
     CDS             2332..3012
                     /gene="pgmB"
                     /locus_tag="KR250_003511"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HBH0069394.1"
                     /translation="MIEAFIFDLDGVITDTAYYHYMAWRKLAHKVGIDIDTKFNESLK
                     GISRMESLDRILEFGNKKYSFSEEEKVRMAEEKNNYYVSLIDEITSNDILPGIESLLI
                     DVKSNNIKIGLSSASKNAINVLNHLGISDKFDFIADAGKCKNNKPHPEIFLMSAKGLN
                     VNPQNCIGIEDASAGIDAINSANMFSVGVGNYENLKKANLVVDSTNQLKFEYIQEKYN
                     EYIVRRII"
     gene            3009..5294
                     /locus_tag="KR250_003512"
     CDS             3009..5294
                     /locus_tag="KR250_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HBH0069395.1"
                     /translation="MKCLFSNVDEWKIIQDKIEIKENRLAESIMSIGNGYMGMRGNYE
                     ENYSGDTHKGSYIAGVWFPDKTRVGWWKNGYPEYFGKIPNSVNYIGVRIFIDDVELDL
                     AKCKVEDFYRELDMKNGILNRRFIVNINDKKFEVRVARFLSISVKELAVIKYSIKSLN
                     SNTKIKFVPYLDSNIKNEDSNYEEKFWNGISSSSLEYKGSILSRTKENAFNTPVFTVG
                     SMMYIKTNGDVEAVSHESDYNYCENSITMDVLKNEVASIEKYVSITSTRDYKESELLR
                     KCEDILNRELSKSYDDILNEHTKLWNKRWESADVKINGDKSSQQGIRYNLFQLFSTYY
                     GEDSRLNIGPKGFTGEKYGGATYWDTEAFCVPVYLGVADKEVTRNLLEYRYNQLEQAK
                     DNAKKLGLKGALYPMVTFDGVECHNEWEITFEEIHRNGSIVYAIYNYTNYTGDYKYIK
                     EKGIDVIIEIARFWASRVHLSTKKDLYMIHGVTGPNEYENNINNNWYTNYIAKWCLDY
                     AVENILRLEKECNECIDRNNVTREEVEFWKIISNKMYLPYDEELQIIIQNDNFLDKEF
                     VEVKDLPKENFPLNQNWSWDKILRSCFIKQADVLQGIYYFGNRFTKEEKKRNFDFYEK
                     YTVHESSLSSSIYSIIASEIDNLEKAYELYSRTARLDLDNYNNDTNDGLHITSMSGSW
                     LSIVHGFAGMRTWNETLSFDPKLPKEWKNYSFNINYRDNIINVDVCSDLIKIENLKGR
                     PIKIIVYGKKYILEKCIKIDK"
     gene            5519..6412
                     /locus_tag="KR250_003513"
     CDS             5519..6412
                     /locus_tag="KR250_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HBH0069396.1"
                     /translation="MKNKKFIVIILIISILIGVLVKEYSSREIKKDDINVSKYIKYAD
                     LASKNNAQVNWKYVASIVAVLNKNNLKNVKDSQIQEVSDLFVKNFSKNNKINKLSDIL
                     DELEFSNRQKRLVDNYIDNLKDYGIKPERLKSDTKYMKFIDEIKTEAIQNYKDYKILP
                     SITIAQAIIESSWGKSTLAKQYSNLFGIKADAYWKGKSVTLETKEHLDTIIDDKFRIY
                     DDKNESIKDHAKFLATNKRYKNNGVFDAKTYIYQAKALEKAGYSTAKDENGNSIYAAR
                     LIELIQQYNLQLIDSEIQSEM"
     gene            6849..7340
                     /locus_tag="KR250_003514"
     CDS             6849..7340
                     /locus_tag="KR250_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069397.1"
                     /translation="MYENNKVIYNLERCEKLEKTLYLSQFNTYKNFKLASKKTVINSL
                     ETYVYNMNINNISLVDTIECISLEDEQLTGKKLILVGDIDIDAILDYAGNKRNRNSSK
                     KNTFKLKIPFSTFIQMPRKIENKDKINLKYLIQDITSSILDDNLFISVTAIISYENSS
                     KIE"
     gene            7368..>7782
                     /locus_tag="KR250_003515"
     CDS             7368..>7782
                     /locus_tag="KR250_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069398.1"
                     /translation="MRITKTTFNDVFSSDANESIIKVEKYVDKKCASVFEILTYTIIV
                     TNTNRYKTGNIFFKDYISKYIEFINNTVKVNGIIKRGLDPQQGFYIGRIDASCKKIIS
                     FKSVVLPNSAHRIIKNSANIYYYYKCNLDKFPTRIS"
BASE COUNT         3258 a          692 c         1336 g         2496 t
ORIGIN      
        1 aagttaatag ttataatatt gtatcctcta gaaaaatact agaggttttt attttgctaa
       61 aataactaaa tagaattaaa tttaaatagt atagtttatt ttaaattgaa tagtttattg
      121 ttgatatatt ttactattat atatttttat tgtgatataa tagtaaaata tattaaccta
      181 tctttagatt atataaatta ttataaagga gggaaatttt aaaatgtcta ataaaaataa
      241 agaaaatata actttaagta atcaaatcaa aggaacaatt caaaatagtt taacaaaaga
      301 agctatatta catataccaa tgagcaatta tgcctatggt tatgatagag aaacactaca
      361 tataagactt agaactaaaa aaaatgaagc gaaaagagta attttacgaa taggagacca
      421 atatgtgtgg gataaaggag gtgcaggagg aggaaattta aatgcatctg gagttggttg
      481 gtctggtgga acaaatatag taatgagtaa agaagtagaa actgaattat ttgactattg
      541 gatagctaag tgtaagcctt taaataaacg ctcacgatat ggctttataa tagaaggtca
      601 agaggaaaaa cttttattta cagaaaaaag aataatagag ttgggaaatg aaaatgatga
      661 aaaggaatta tgtgaaatag gtaatttttt tggatatcca tatttaaatt atatagatat
      721 acctaaagtg ccaaattggg tgaaagatac tgtttggtat cagatatttc cagatagatt
      781 tgcaaatggt gacccgtcta taaacccaga aggagtagat aaatggggag ctattccaac
      841 cagagataat tttacaggtg gagatttaca aggtgtgata gaacacttag attatttgag
      901 tgatttggga ataaacggaa tttacttttg ccctattaca attggaaaaa ccaatcatag
      961 atatgatacg gtagattata tggaggtaga ccctacatta ggagataaag agactttaaa
     1021 aaaactaata gaagaagcgc ataagagaaa tataaaaata atgttggatg ctgtttttaa
     1081 tcatatagga tattattcta aacagtggca agatgtagtt caaaataagg aaaattctcg
     1141 atataaaaac tggttttata taaaagatat gagtaaaatt gatactccaa tagaacaaat
     1201 agatgaaaaa aatatacctt atgaaacctt tgggtgtgaa aaatatatgc ctaaacttaa
     1261 tacagaaaat tcagaagtaa taaaatattt attggatgta ggcaagtact ggatacagga
     1321 atttgatata gatgcgtgga gattagatgt ttcaaatgaa gtagaccatg ttttttggag
     1381 aaagtttaga gaagaagtca aaaaagtaaa accagacatt tatatattgg gagaaatttg
     1441 gcatggtagt ttaccatggc taatgggtga ccaatttgat tcagttatga attacttgat
     1501 gtcagaagcc atgaaaaaat tcttttgtac agatgagata aacgccgaag aatttaagta
     1561 tatgataaat gatgtaatgg taagttatcc aatacaagtt agtgaagtta tttttaattt
     1621 acttggaagt cacgatacta caagaatatt gacttatgct aatggaaata ttgatagatt
     1681 taaacttgca tatatgttta tgtttgtaca aacaggatgt ccttgtatat actatggtga
     1741 tgaaatcgga atggaaggag aattaagttc aatatctgaa ggtcaaagaa aatgtatgga
     1801 atggaatgaa gaaaaatgga ataaggatat atttgatttt atgaagaaga ttattagact
     1861 tcgtaaagaa aataaagaac ttaggtctat atcaaatgaa tgggtgttag cagataagga
     1921 aaatggaacg attatattga aaaaagaatc tatttctata ataataaata attcatctaa
     1981 aaatataatt ttgacgttac cagattattt agcaaataaa aaagtaaaag atttatatga
     2041 agaagaaata gttgatttaa aagaagaact aatgttagag aaatacaagt tcattatttt
     2101 aaaataatac aaaaaagtat ttattttaga tttcatgtta aattattata cagtttaaat
     2161 aagtctttat aaacgataaa aaaatatggt ctacaagtga gtgaattgaa agtagatttt
     2221 aaattaaaga ctgagttaga gtagataatt ttataaataa tatcttaaat acaagataga
     2281 aaataaattg attattagtc taaaaatttc ttgtagaaag aggaaataag aatgattgaa
     2341 gcatttatat ttgatttaga tggtgtaatt acagatactg catattatca ctatatggct
     2401 tggagaaaac tagcccataa agttgggata gatatagaca caaaatttaa tgaatcttta
     2461 aaaggcataa gtagaatgga gtcattggac aggatattag aatttggaaa taagaaatat
     2521 tcattttcag aagaagaaaa agtgagaatg gctgaagaaa aaaataatta ctatgtatca
     2581 ttgatagatg agataacttc aaatgatatt ttgccaggta tagaaagctt gttaattgat
     2641 gtaaaatcta ataatataaa gataggtcta tcttctgcaa gtaagaatgc aataaatgta
     2701 ttaaatcatt tgggtataag tgacaaattt gattttatag ctgatgcagg aaaatgtaaa
     2761 aacaataaac cacatccaga gatatttctt atgtcagcga aagggcttaa tgtaaatcca
     2821 caaaattgta tagggataga ggatgcaagt gcaggtatag acgctataaa ctcagctaat
     2881 atgttttctg ttggtgttgg aaattatgaa aacttaaaaa aagcaaattt agttgtagat
     2941 tcaacaaatc aattaaagtt tgaatatata caagaaaagt ataatgagta tatagttagg
     3001 agaattatat gaaatgttta tttagtaatg tagatgagtg gaagattata caagataaga
     3061 ttgaaatcaa agaaaataga ttagcagagt ccataatgag tataggcaat ggatatatgg
     3121 gtatgagagg aaactatgag gaaaattata gtggagatac acacaagggg tcttatatag
     3181 ctggagtatg gtttccagat aaaactcgtg ttggttggtg gaaaaatggg tatccagaat
     3241 actttggtaa aattcctaat tcagtcaact atataggtgt aagaattttc atagatgatg
     3301 tagaattaga tttagcaaaa tgtaaagtag aagattttta tagagaatta gatatgaaaa
     3361 atggtatttt aaatcgtagg tttattgtaa atataaatga taaaaaattt gaagttaggg
     3421 ttgctaggtt tttaagtata tctgttaagg aacttgcagt aattaaatac agtataaaat
     3481 cattaaattc taatactaaa ataaaatttg taccatattt agattcaaat ataaaaaatg
     3541 aagattcaaa ctatgaggaa aagttttgga atggaatttc ttcaagttct ttagaatata
     3601 aaggtagtat tctttctcga acaaaggaga atgcatttaa tacaccagta ttcactgtgg
     3661 gttcaatgat gtatattaag acaaatggtg atgtagaagc tgtatctcat gagagtgatt
     3721 ataattactg tgaaaattcg ataacaatgg atgttttaaa aaatgaggtt gcaagtatag
     3781 aaaaatatgt atctataact tcaactagag attataagga aagtgagcta ttaagaaaat
     3841 gtgaagacat tttaaataga gaactatcta aaagttatga tgatatatta aatgaacata
     3901 caaagttatg gaataaaaga tgggaaagtg cagatgtaaa aataaatgga gataaaagta
     3961 gccaacaagg tataagatat aatttattcc aactattttc aacatattat ggagaagatt
     4021 cgcgattaaa tataggtcca aaaggattta ctggtgaaaa gtatggagga gctacttatt
     4081 gggatacaga agcgttttgt gttccagtat atctaggtgt agcagataag gaggtaacta
     4141 gaaatctttt agaatatagg tacaatcagt tagaacaggc aaaggataat gcaaaaaaac
     4201 tagggcttaa aggtgcttta tatccaatgg taacatttga tggagtagaa tgtcataatg
     4261 aatgggagat aacatttgag gaaatccacc gaaatggaag tatagtatat gcaatatata
     4321 actatacaaa ttatacaggt gactataaat atataaaaga aaaaggaata gatgtaataa
     4381 tagaaatagc tagattttgg gcaagtagag ttcatttgtc tacaaaaaaa gatttgtata
     4441 tgatacatgg agtaacaggt ccaaacgagt atgaaaacaa tatcaataat aattggtata
     4501 ctaattatat agctaaatgg tgtttagatt atgctgtaga aaatatctta cgattagaga
     4561 aagagtgtaa tgaatgtatt gacagaaata atgtgactag ggaagaagtt gagttttgga
     4621 aaataatatc aaataaaatg tatctgccgt atgatgagga actacaaata ataattcaaa
     4681 atgataattt tttagataaa gaatttgtag aagtaaagga tttaccaaaa gaaaattttc
     4741 ctcttaatca aaattggtct tgggataaga ttcttaggtc gtgttttata aaacaagcag
     4801 atgtattaca gggaatttac tattttggaa atagatttac taaagaagaa aaaaagagaa
     4861 attttgactt ctatgaaaaa tatactgtac atgaatcttc tttatcttct agtatttatt
     4921 ctattattgc ctcagaaata gataacctag aaaaggctta tgaattatat tcaagaacag
     4981 ctaggcttga tttagataat tacaataatg atacaaatga tggactgcac ataacttcaa
     5041 tgtctggttc atggctttcc attgtacatg gatttgcagg catgagaaca tggaatgaaa
     5101 ctctatcttt tgaccctaag ttgccaaaag agtggaaaaa ttattctttc aatataaatt
     5161 atagagataa tattattaat gtagatgtat gtagtgattt aattaaaata gaaaatttaa
     5221 aaggtagacc aataaaaatt attgtatatg ggaaaaaata tatattagaa aaatgtataa
     5281 aaattgataa atagatttaa taatattttt attatataaa tagttaaaat aagctatcta
     5341 attatttatt tattttaact attttgtgtg atagaaaact atatattata acaatagtag
     5401 tgcttttaat taaatatatt tcataaatct tcataaaatc ttcaaaattt cgtaaagaat
     5461 atgtcacaaa agtaaggtat attatattca gcagatagta taagggagga caatatttat
     5521 gaagaataaa aaattcattg ttataatact gataatatca attttaatag gtgtattagt
     5581 gaaggaatat tcatctagag agataaaaaa ggatgatata aatgtcagta agtatataaa
     5641 atatgctgat ttagcaagta aaaataatgc acaagttaac tggaagtatg tagcttccat
     5701 tgttgctgtg ttaaataaaa ataacttaaa aaacgttaag gattcacaga tacaagaagt
     5761 atcagattta tttgttaaga atttttctaa aaataataaa ataaataagt taagtgatat
     5821 tttggatgag ttagaattta gtaatagaca aaaaagattg gttgataatt atatagacaa
     5881 tcttaaggat tatggtataa aaccagaaag attgaagtct gatacaaaat atatgaagtt
     5941 tattgatgaa attaaaacag aagcaataca aaattataag gattataaaa tacttccatc
     6001 tataactatt gcacaagcta taatagaatc tagctggggc aagtcaactt tggcaaaaca
     6061 atatagcaat ctatttggta taaaggctga cgcttattgg aaaggtaagt cagtgacttt
     6121 agaaacgaaa gaacatttag atacaattat agatgataaa tttagaatat atgatgataa
     6181 aaatgaatct ataaaagacc atgcaaaatt tttagccaca aataaaaggt ataaaaacaa
     6241 tggtgtattt gatgcaaaaa cgtatatata tcaagctaaa gcattagaaa aggcaggtta
     6301 tagtactgcg aaggatgaaa atggcaatag tatatatgca gctcgtctaa tagaattaat
     6361 tcaacagtat aatcttcaat taatagacag tgaaattcaa agtgaaatgt aactttgatt
     6421 aaatttttat tgataaggct atgattttta aaatcatagc cttattttga catgctttta
     6481 cttgtaatta ttaaatcgat atcattatct ttcaaaattt actttatact gtaaatcaaa
     6541 ttatttaagt taaaacttgg aattatttat aaaaggatta tttactttat ctaaattctg
     6601 cacttaaaga acatcttttc ccttaagact gaccgttagt ctataaaaat tataattact
     6661 tataaaaaag tgaatatata tataaagttt tcagaaagac ttttacttgt aaattgaaat
     6721 caatgaagta aaaaaggatt ctttcttaaa agtaaataac aactagatag tgaataataa
     6781 aaaatggtaa tttgaaataa tttttatgaa tagtattaat atatagatac agggagattg
     6841 atgcgattat gtatgaaaat aataaggtaa tatacaacct tgaaagatgt gaaaaactag
     6901 aaaagactct ttatttatct caatttaata catataaaaa ttttaaatta gcttctaaaa
     6961 aaactgtaat aaattcatta gagacatatg tttacaatat gaatattaac aatataagtc
     7021 ttgttgatac tatagaatgt atatcattag aagatgaaca acttacagga aagaagttga
     7081 ttttagttgg tgatatagat attgatgcaa tattagatta tgcaggaaat aaaagaaata
     7141 gaaattctag taaaaaaaat acatttaaat taaaaatacc ctttagtaca tttatacaaa
     7201 tgcctagaaa gatagaaaat aaagataaaa ttaatttaaa gtatcttata caagatatta
     7261 cttcatctat tttagatgat aatttattta ttagtgttac tgcaattata agttatgaaa
     7321 atagttccaa gatagagtaa tctttaaatt atagaaaggt ggatgcaatg agaataacga
     7381 aaactacttt taatgatgta ttttcaagtg atgcaaatga aagtattata aaagtagaaa
     7441 aatatgttga taaaaaatgt gcatctgtat ttgagatttt gacctacact attattgtta
     7501 caaatacaaa taggtacaaa actggaaata tatttttcaa agattatatt tctaaatata
     7561 tagagtttat aaataatact gtaaaagtta atggcattat aaaacgtgga ttagacccac
     7621 agcaaggttt ttacataggg agaatagatg caagttgtaa aaaaataata tcttttaaga
     7681 gtgtagtttt accaaattct gcacatagga ttataaagaa tagtgctaat atatactatt
     7741 attataagtg taatctagat aaattcccaa caagaatatc aa
//
LOCUS       DAEPIN010000120         7751 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.122, whole genome shotgun sequence.
ACCESSION   DAEPIN010000120 DAEPIN010000000
VERSION     DAEPIN010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7751
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.122"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            <1..377
                     /locus_tag="KR250_003516"
     CDS             <1..377
                     /locus_tag="KR250_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069399.1"
                     /translation="KGSNLKLCNLIVFGCIEVEIDYSCKNRSNFKTVNKSNDIKKDKY
                     YKNDIQNNNENINDCKKQISFGDNKLKKITKTFGFSCFICSPVGIIYEDMKNINIKIE
                     HTSINELNPEELFVNTSLLLYY"
     gene            667..1209
                     /locus_tag="KR250_003517"
     CDS             667..1209
                     /locus_tag="KR250_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBH0069400.1"
                     /translation="MKICITVGHSILKGGKSTGVNGIVDEYNYNKKLAPMLAEMLISQ
                     GNTVDVIICPERHFMSEREEFFYRVPKVNSGKYDLLVELHLNKADGTQCGTEVLYYGT
                     EGLEYAKRVSNRLGELFENRGAKKRENLYILRNTNPVAIQIESFFCDNVNDCNKANES
                     GYDYIARLITEGILNKDINM"
     gene            1537..3030
                     /locus_tag="KR250_003518"
     CDS             1537..3030
                     /locus_tag="KR250_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBH0069401.1"
                     /translation="MKYTFEKFKLNEKILKSLKSLGYNIPSRVQREVIPKLLKGQNLV
                     VRSKTGSGKTASFAIPLCENINVDYNNIQALIVVPTRELALQVKDEISDIGRLKKVRC
                     SAIFGKQSIKDQIAELKQRVHIVVATPGRILDHINRGSIKLENVKYLVIDEADKMFNK
                     GFVEQMEKILLNLPKEKIVSLFSATIDEEIKYICEKYMLDYSVINIEENESDTNQKTR
                     QIDDKIIKANGREKYILLKELIYSENPKSVIIFCNTKEKVSKLYNKMSKEGFLIRELH
                     ADLSQERRIFVIKDFKNQKFNVLVSSDVASRGIHIDDISLVINYDVPQDKENYIHRIG
                     RTGRKGNSGKAITIVTEKDEKYIENIETYIGYKINELKDIEKSRITHGKILFEEYSKG
                     ILKKVSKRKKVDKNSYNSKDIKLNIESEVVKLYLNAGKKKKIRVLDIVGAFSNIKGIT
                     NDDIGVIEVQDLCSYVDILNYKGDLILKKYKEIPIKKKMVKVKRDIR"
     gene            3104..3337
                     /locus_tag="KR250_003519"
     CDS             3104..3337
                     /locus_tag="KR250_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069402.1"
                     /translation="MKAEDMKRKVSPIDLERILVTGGVIKHEDSIWLLDFWGNKDIAG
                     ILLMPPTRHVILHLNDCCKWKEYFRTKKKFYRS"
     gene            3493..4257
                     /locus_tag="KR250_003520"
     CDS             3493..4257
                     /locus_tag="KR250_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBH0069403.1"
                     /translation="MAYFNYNDKQCYYNEIGDGTPLLFLHGNTASSKMFNDIIDFYKD
                     EYKVILIDFVGHGKSQMVDKFSADLWFDEAMQVICFLEAMNYKKVNIIGSSGGALVAL
                     NVALERPDLVNKVIADSFEGEVPLESFVQNVKIEREASKQDDGAKAFYIYNQGENWEK
                     VVDNDTEAIFEHYKTIGKFFHKPLETMQPEVLLTGSREDEFVSLISSDFFENTFSLLL
                     EKIKNGKMYLFDKGGHPAILSNGLDFSNVAKKFLEE"
     gene            complement(4542..7721)
                     /locus_tag="KR250_003521"
     CDS             complement(4542..7721)
                     /locus_tag="KR250_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBH0069404.1"
                     /translation="MKIDMIYKLIRRNTEPSRWSRGVKYYTNQLVDYIDIDIDDLQNN
                     LLTFRGGVNSEDNYSFYFNSITIDIKHFKIIENDCSCADCQSKGKSIFICKHIVAIVI
                     KSLKQIKNKKDVPPILIDILGADKDKLSNTNSKTSLDLSKQLLSLLDNTKRERVNLDV
                     LINVDSNKAIAEFKIGIDKMYILKNITEFAYSKLNGYELDFGKKFTYNPNTHYFADED
                     EKLVDIIEEYGTNLSYNFSSSHSKKFMEINASSLKQFLNAIKYKDFTLIFSKEEYHPK
                     ILNDTLPVRFDFKNIEDKIVLYSKDSLPVPLTKKNDVLLYDGNIYLLSHKKAHDYSKI
                     YQILSKTKELKFDKEDSKDVLGLLVPRLKSISQEVHLDDNIKNNITKDFKSEFYFDMI
                     DGNICCDVKYIYDDEDKKFVLPNIQKEATIENKLTSSQFTKENNHYVFKGTDHDLFTF
                     LDLQLESLKEFGDIYYSEKFKLKKIYNASSIQASINQTNQNYLEFNFNISDINPREYK
                     DILKAFQEKRTFYKLKDGNFIDLSERETKDFFELVENLDIMDKSKNNKIHKNKALYIN
                     DVIRSKNLKFIDGKKELDSICEKFRNFDSINLEIPANLNANLRDYQINGLNWFKVLDY
                     YKFGGILADEMGLGKTIQTIAFLLSLSNKKSLIVTPTSLIYNWKNEFEKFAPDIKVLL
                     IHGNKRDREKCFMELENFDVILTTYGTLRNDLEKYSEIKFDYCILDEAQNIKNPVALV
                     TESVKSINAENKFALTGTPMENNLLELWSIFDFIMPGYLYSKAKFQELFINKEDNVKN
                     LKKLIKPFILRRSKKQVMKELPDKIEKNFFVELNKEQKKIYSVYSKDIQDKMKDKNLK
                     KDKIVIFSYLTKLRQLCLDPSIVVKDYNKKSSKIETCLEILRDSINENHKILLFSQFT
                     SVLKNISKELDKYKIKYHYIDGKTNAKERLELVDEFNNSMDKKVFLISLKAGGTGLNL
                     TSADMVIHFDPWWNPSVENQASDRAHRFGQKNSVQVIKLIAKGTIEEKIIKLQESKKE
                     LINQFINGELSNEGVLKSLSDEEIINLFN"
BASE COUNT         2971 a          835 c         1112 g         2833 t
ORIGIN      
        1 acaaaggtag taatttgaaa ttatgtaact tgatagtttt tgggtgtata gaagttgaaa
       61 tagactatag ttgtaaaaat aggagtaatt ttaaaacagt aaataagtct aatgacatta
      121 aaaaagataa gtattataaa aatgatatac aaaataacaa tgaaaatata aacgattgta
      181 aaaagcaaat aagttttggt gataataagc taaaaaaaat aacaaaaact tttggatttt
      241 cttgtttcat ttgttctcca gttggaatta tttatgaaga tatgaaaaat ataaatataa
      301 aaattgaaca tacatcaata aatgaattaa atccagaaga attatttgta aatacatcac
      361 ttttattgta ttattaagta ttttaaagct aaaacttaaa cttattaaaa tagcatttgg
      421 ttaaaggaaa atagatattg atatatttat catatatata aatgattaat taaacaatta
      481 taaataataa gtatattata atatttaaaa aatatagatt tatgataaaa taagacaaat
      541 atgttaaaaa aatgataatc acggagaact tttaccttct tcaattaata tattattatt
      601 agtaataact ttaaaattat attactatta tataaaaatt ggcttaaaag aaggtgaaaa
      661 aagtaaatga aaatatgcat aacagtaggt catagtatac taaaaggtgg taaaagtaca
      721 ggtgtaaatg gtatagttga tgaatacaat tataataaaa aattagctcc tatgttagct
      781 gagatgctta taagtcaagg taatacagta gatgtaataa tttgtccaga aaggcacttt
      841 atgtcagaaa gagaagaatt tttctataga gttccaaaag taaacagtgg taaatacgat
      901 ttattagtag agctacattt aaataaagct gatggaacac aatgtggtac agaagtgctt
      961 tactatggca ctgaaggatt agaatatgct aaaagagtat ctaatagact tggagaatta
     1021 tttgaaaata gaggagctaa aaaaagagaa aatttatata tattgagaaa tacaaatcca
     1081 gttgcaatac agattgaaag ttttttctgt gataatgtga atgattgtaa caaagcaaat
     1141 gaatcaggtt atgattatat agcacgattg attacagaag gtatattgaa taaagatata
     1201 aatatgtgag attatggtga tttaggtatt gattaatacc ttttaatatt agatataaat
     1261 aaaaatacaa tgtattagta taatttaaac tttcaaaaga tagattagtt tgattattga
     1321 caagagatac ctccttgaaa atattgattt atcagtatat ttaaggaggt atttaaatat
     1381 aaaaatttaa ttaatttgct actaaactat aggtttatag agtatatatt tttatcttca
     1441 gtctaataaa taatataaaa atagtagaac cttgtattat aataatataa tgtagaaaaa
     1501 tatatagata agataaatat aataaggtgg taatatatga aatatacatt tgaaaaattt
     1561 aagttaaatg aaaagatact taagtcattg aagagtttag gatataatat accttctaga
     1621 gtacaaagag aagttattcc aaaattatta aaaggtcaaa acttagtagt taggtcaaaa
     1681 acaggaagtg gaaagacagc aagttttgca atacctttat gcgagaatat aaatgtagat
     1741 tacaataata ttcaagctct tatagtggtt ccaacaagag aattagcctt gcaagtaaaa
     1801 gatgagatat cagatatagg gagactaaag aaggtgagat gtagtgctat atttggtaaa
     1861 caatctataa aagaccaaat agctgaattg aaacaaaggg ttcatattgt tgtagctact
     1921 ccaggcagaa tacttgacca tataaataga ggttctatta agttggaaaa tgtaaagtat
     1981 ttagtaatag atgaggcaga taaaatgttt aataaaggtt ttgttgaaca aatggaaaaa
     2041 atattattaa atttacctaa ggagaaaata gtaagtcttt tttcagctac aatagatgaa
     2101 gagataaaat atatatgtga aaaatatatg ctagattaca gtgttataaa tatagaagaa
     2161 aatgagagcg atactaatca aaaaacaaga caaatagatg acaaaataat caaggcaaat
     2221 ggaagagaga agtacatcct tttaaaagaa ttaatataca gcgaaaatcc aaagagtgtg
     2281 ataatatttt gtaataccaa agagaaagta tctaaattat ataacaagat gagtaaagaa
     2341 ggttttttaa taagagagtt acatgcagat ttatcacaag aaagaagaat ttttgtaatt
     2401 aaggatttta aaaaccaaaa atttaatgta ttagttagta gtgatgtagc gtccagagga
     2461 atacatattg atgatatttc tttagtaata aattatgatg tgccacaaga taaagaaaac
     2521 tatatacata gaataggtag aactgggaga aaaggaaata gtggaaaggc aattactata
     2581 gtaactgaaa aggatgaaaa atatatagag aatatagaaa cgtatatagg atataaaata
     2641 aatgaattaa aagatataga aaaaagtaga attacacatg gcaaaatttt gtttgaagaa
     2701 tattcaaaag gaatcctaaa aaaagtatca aagagaaaaa aagttgataa aaattcttac
     2761 aatagtaaag atataaaact aaatatagaa agtgaagttg ttaaattata tttaaatgct
     2821 ggtaagaaga agaaaataag agttttagat atagttggtg catttagtaa tataaaaggt
     2881 ataactaatg atgatatagg tgtaattgaa gttcaagatt tatgttcata tgtagatata
     2941 ttaaattata agggagattt gatattaaaa aaatataagg agatacctat aaagaaaaaa
     3001 atggttaaag tgaaaagaga tatacgataa tttagtcaat attgatgaat tgctttgtta
     3061 tcgaaatgaa gttgcaaaaa acacaataga agttgttaag gatttgaaag ctgaagatat
     3121 gaaacgaaag gtctctccaa ttgacttaga aaggattttg gtgactggag gagtaataaa
     3181 gcatgaagat tctatttggt tgttggactt ttgggggaat aaagatattg ctggaatttt
     3241 gcttatgcct cctactcgtc atgttatact tcatttgaat gattgttgta aatggaagga
     3301 atattttcgt acaaaaaaga aattttacag aagttaaatt ataataaatt agacaaacaa
     3361 tagataaatt agcaaggaat gttgatttta tctacattct aagttatact atttttaagt
     3421 ggctaatgga atagtcattt taaatataag taataaatat atacctatag ggatattaaa
     3481 ggggggaaat atatggcata ttttaactat aacgacaaac agtgctacta taatgaaatt
     3541 ggggatggta caccattact ttttttacat ggaaatacag cctcttccaa gatgtttaat
     3601 gatataatcg atttttataa agatgaatat aaagtaatat taattgattt tgtaggtcat
     3661 ggaaaatctc aaatggtcga taaattttct gctgacttat ggtttgatga ggctatgcaa
     3721 gtgatatgtt ttttagaagc aatgaactat aaaaaagtaa acataattgg tagtagtgga
     3781 ggagctcttg ttgcattaaa tgttgcactt gagagacctg atttagtgaa taaggtaatt
     3841 gcagatagtt ttgaaggaga agttcctcta gagtcatttg tacaaaatgt gaaaatagag
     3901 cgtgaagcat ctaagcaaga tgatggtgca aaagcttttt atatttataa tcaaggagaa
     3961 aactgggaaa aagttgtaga taatgataca gaagctattt ttgaacatta taaaactata
     4021 ggaaaatttt ttcataagcc tctagaaact atgcaaccag aggtgctttt aacaggaagt
     4081 agagaagatg agtttgtatc tctaatatct agtgattttt ttgaaaatac tttttcatta
     4141 cttcttgaaa agattaaaaa tggtaaaatg tatttgtttg ataaaggtgg tcatcctgca
     4201 attttatcga atggattaga tttttcaaat gtagcaaaga aatttttaga ggaataattt
     4261 aaccaaaatt tataataatg aaaactgtaa aatggattgc aaaacttaca aaagtgagtg
     4321 tacatatact tcattattat gatgaaatag tattgttaaa acaaaatatt ctaatagtca
     4381 agttattaca tttgtatatg caatatgtat agaaagagat atcattagat tgtaaagttg
     4441 ggattaaata acaatattaa attttattta ttcagatatt ttagcttaac tatataaatg
     4501 ttagaacaaa tttatatagt taagctgtag gattaaataa tttagttaaa taagtttatt
     4561 atctcctcat cagatagact ttttaataca ccttcattag aaagttctcc atttataaat
     4621 tgatttataa gctctttttt actttcttgt aattttatta ttttttcttc tatagtccct
     4681 ttagcaatca attttataac ttgtactgaa tttttttgac caaatctatg agctctatca
     4741 ctagcttgat tttctactga tggattccac catgggtcaa agtgaattac catgtcagca
     4801 gatgtaagat ttaaaccagt tccgcctgct tttaaagata ttaaaaaaac ttttttatcc
     4861 atactattat tgaactcatc tacaagttca agtctttctt tggcattagt tttaccatct
     4921 atataatggt atttaatttt atacttatca agttccttac ttatgttctt aagcacagaa
     4981 gtaaattgtg aaaacaataa tattttgtgg ttttcgttta tactatccct aagtatttct
     5041 aaacatgttt caatttttga acttttttta ttgtaatctt ttactactat acttgggtct
     5101 aaacaaagtt gtcttagttt agttaaataa gaaaatataa ctattttatc tttctttaga
     5161 tttttatctt tcattttatc ttgtatatct tttgaatata cactatatat ctttttctgc
     5221 tctttattca attcaacaaa aaagtttttt tcaatcttat ctggaagttc tttcataact
     5281 tgttttttac ttcttctaag aataaaaggt tttatcaatt ttttcaggtt tttaacatta
     5341 tcttctttat ttataaacaa ttcctgaaac tttgctttac tatatagata gccaggcatt
     5401 ataaaatcaa atatagacca taattctagt agattatttt ccataggtgt accagtgagt
     5461 gcaaatttat tttctgcatt tatacttttt acagactcag ttactaaagc tactggattt
     5521 tttatatttt gagcttcatc taatatacaa taatcaaact ttatttcact atatttttct
     5581 aaatcatttc taagagtacc atatgttgta agtattacat caaaattttc taattccata
     5641 aaacattttt ctctatctct tttatttcca tgtatcaata aaacttttat atctggagca
     5701 aacttttcaa attcattttt ccaattataa atgagtgatg tcggtgtaac aataagactt
     5761 tttttattag ataatgataa taaaaatgca atagtctgta ttgttttacc aagacccatt
     5821 tcatcagcta atataccacc aaatttataa taatctaaaa ctttaaacca attgaggcca
     5881 tttatttgat agtcacgaag gtttgcattt aaatttgcgg gaatttctaa atttatgcta
     5941 tcaaaatttc taaatttttc acatatactg tctagttctt ttttaccatc tataaacttt
     6001 aaatttttac tacgtattac atcatttata tatagagctt tatttttatg aattttatta
     6061 ttttttgatt tatccattat atctagattt tcaactaatt caaaaaaatc ttttgtttct
     6121 ctttctgata aatctataaa gtttccatct ttaagtttat aaaaagtacg tttttcttga
     6181 aaagctttca aaatatcctt gtactctctt ggatttatat cagatatatt aaaattaaat
     6241 tctaggtaat tctggttagt ctgattgatt gaagcttgaa ttgatgaagc gttatatatt
     6301 tttttcaatt taaacttctc agaataatat atatctccaa attcctttag agattctaat
     6361 tgtaaatcta aaaatgtaaa taggtcatgg tcagttcctt taaacacata atgattattt
     6421 tcctttgtaa attgtgaaga agtaagttta ttttcaatag tagcttcctt ttgtatgttg
     6481 gggagcacaa attttttatc ttcatcatca tatatatact ttacatcaca acatatgttg
     6541 ccatcaatca tatcaaagta gaactcagat ttaaaatctt ttgttatatt attttttatg
     6601 ttatcatcta aatgaacttc ttgagatata gattttaatc taggaactag caatcctaaa
     6661 acatccttag aatcctcttt atcaaatttt aactctttag tctttgataa tatttgatat
     6721 atttttgaat aatcatgagc ttttttatgt gataataaat agatattgcc atcatataaa
     6781 agtacatcat tttttttagt aagtggaaca ggtagtgaat ctttagaata taagactatt
     6841 ttatcttcta tatttttaaa atcaaacctt acaggtagag tatcatttaa tatctttgga
     6901 tgatattcct ctttagaaaa aataagggta aaatccttgt acttaatagc atttaaaaat
     6961 tgttttaaag agcttgcatt tatctccata aatttttttg aatgtgatga agaaaagttg
     7021 tatgaaagat ttgtcccata ttcttctata atatctacta acttctcatc ttcatcagca
     7081 aaataatgag tgttggggtt gtaagtaaat ttcttcccaa aatctaattc atatccatta
     7141 agttttgaat atgcaaattc agtaatgttt tttaaaatgt acattttatc aattcctatt
     7201 ttaaattcag caattgcttt atttgaatcc acatttatca atacatctaa attaaccctt
     7261 tctcttttag tattgtctaa caaagaaagt aattgcttgc ttaaatctaa gctggtttta
     7321 gaatttgtat tagaaagctt atccttatct gcacctaata tatctattag aataggagga
     7381 acatcttttt tattttttat ttgttttaag ctttttatca caatagcaac tatatgctta
     7441 catataaaaa tacttttgcc ctttgattga cagtcagcgc aactacaatc attttcaatt
     7501 atcttaaagt gctttatatc tatagttatt gaattaaaat aaaagctata attatcttca
     7561 gaattgactc caccacgaaa tgtcaacagg ttattttgta ggtcatcaat atctatgtct
     7621 atataatcaa ctaattgatt agtataatat tttacacctc ttgaccatct tgatggttct
     7681 gtatttcttc ttattagttt atatatcata tctattttca tattattcac cttacctcac
     7741 tgtatatatt a
//
LOCUS       DAEPIN010000121         7725 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.123, whole genome shotgun sequence.
ACCESSION   DAEPIN010000121 DAEPIN010000000
VERSION     DAEPIN010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7725
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.123"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            196..507
                     /locus_tag="KR250_003522"
     CDS             196..507
                     /locus_tag="KR250_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HBH0069405.1"
                     /translation="MYFTYIIRCKDDSLYTGYTSNIVRRMNEHKLGINSKYTRAKGFE
                     KLEVYFVTNTKSNAMKLEYYIKKLTRNKKLSIIKNPSILINLIDNKEDYIIGKEIEQL
                     T"
     regulatory      600..692
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            792..1586
                     /gene="thiD"
                     /locus_tag="KR250_003523"
     CDS             792..1586
                     /gene="thiD"
                     /locus_tag="KR250_003523"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HBH0069406.1"
                     /translation="MSNYKIPTLTIAGSDSSGGAGIQADLKTFSAIGTYGMSVITAIT
                     AQNTKGVFAVEDLNKKIIKKQIEAVFEDIPPRAVKIGMVSSPEIILEIVENLKKYNPK
                     YLVVDPVMISKSGYYLLKPEAKENLIKYLIPLAYIITPNIPEAEEITGIKIHNVDDMK
                     RVGEEILQLGPKFVLMKGGHLDGEAVDILVGKNIFKVYKSERIDKKNTHGTGCTLSSA
                     ITSYLALGYEITEAVNLSKIYITEAIKRSFDIGHGVGPVHHFYKFE"
     gene            1610..2398
                     /gene="thiM"
                     /locus_tag="KR250_003524"
     CDS             1610..2398
                     /gene="thiM"
                     /locus_tag="KR250_003524"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HBH0069407.1"
                     /translation="MYNLIKDVKKLNPLVIHYTNNVTINDCANVTLAVGASPLMSFSY
                     EEVEEMVSVANSVVINIGTMNSNMLDLFLLAGKAANKYNKPVVLDPVGVFASKARAEL
                     TSRLLNEVKFSVVKGNVSEIKFIGGFNVRGKGVDSFDEEEDSTEIIRKIAEKLECVVV
                     ATGKIDIITNGKGTYKINNGTDKLKGITGTGCMTASLIASFMAVTENILEAATMGVLT
                     MSLSGELANLNNPPIGTFKENLMNAIYQMDIDTLSKNSNIEFLN"
     gene            2421..3053
                     /gene="thiE"
                     /locus_tag="KR250_003525"
     CDS             2421..3053
                     /gene="thiE"
                     /locus_tag="KR250_003525"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HBH0069408.1"
                     /translation="MIDKESLKKCLKLYLVTDSEMLKGRDFYKCLEDAISSGITTVQL
                     REKNASGREFLRKAMKLREITKQYGVKFIINDRVDIALICDADGVHVGQSDIDVREVR
                     KLIGNNKILGVSARTLEEAICAKNDGADYLGVGSIFTTSTKLDAKSASFETVKEIKEK
                     VDMPFVLIGGINLDNIDKLKCLESDGYAIISAILKAEDISKEVEKWTLKI"
     gene            3192..3494
                     /locus_tag="KR250_003526"
     CDS             3192..3494
                     /locus_tag="KR250_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBH0069409.1"
                     /translation="MIRILLVCVGGMSSTLLVNKMEKDAKKRNIDCKIWAVGEGDIKS
                     ELDNFDILLLGPQLRFMLDDVKSIVGDRAPVSIIDMVNYGTCNGHAVLNSVLEILK"
     gene            complement(3560..4309)
                     /locus_tag="KR250_003527"
     CDS             complement(3560..4309)
                     /locus_tag="KR250_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0069410.1"
                     /translation="MLNILWRSMKWRFKNPISFVVTILQPFLWLVLYSSIANQTMNNI
                     NINNYTAFILPGIIVLVVFSSCSSGGIINFIMKNSGSFYRVLIAPISRYSIVLGQLLE
                     AILVSFIEVTILCIVSIFFSVRIESGIGGILLMIVLIFMTAFFLSSLAYSISLLLPNE
                     IVYETIMTAIVLPIFFLSSALFPIESLSGGLKVAVMLNPFTHVINALRSLIFGETILI
                     GDILPVILLFLILCCSSFSLAMWRLKKEMVS"
     gene            complement(4303..5265)
                     /locus_tag="KR250_003528"
     CDS             complement(4303..5265)
                     /locus_tag="KR250_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0069411.1"
                     /translation="MLAIEIDNLVKEYKNGVKALNGLSFNVNAGEIFSLLGPNGAGKS
                     SLINILTTFYKPTSGNVTMFGKDLVDNPSWIRTQIACVAQQISIDEHLSLMENMVFQS
                     KMYKVEPQIAKQRIDSLIDKFDLSSYLKYPTSSYSGGVKRRLDIAMNMVSSPKILFLD
                     EPTVGMDVDSRKSMWDMLLKIRDEYGTTIFLTTHYLEEAEQLSDNICIMKNGKDLAQG
                     TPSSLRSYIRQNILRITFHNTEDIKKYKDSIKSTGLVKFMSVRENSIFISVNDSRTAF
                     TLINKWLLEHDIEFDAIEIVEPSLEDVFLALTSSKKSLKEEWEC"
     gene            complement(5269..5727)
                     /locus_tag="KR250_003529"
     CDS             complement(5269..5727)
                     /locus_tag="KR250_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HBH0069412.1"
                     /translation="MNCKIEIKNIESVTVATMRYNGPMTEATKYFPNVFKAIKGKSNG
                     APFFCYYDVDQKTGVAEMELCVPTAETPNRMGITTKEFPKTKALFFTHTGSYSNLPKT
                     YEMIFKYIHENNIKIQTPWREVFIKGPGMLIKGNPDNYITEIIFPLKEEE"
     gene            6083..6439
                     /locus_tag="KR250_003530"
     CDS             6083..6439
                     /locus_tag="KR250_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBH0069413.1"
                     /translation="MNFELDNTTPIYLQIVKYIKRQIVTGELKPGETIPSRREMALNL
                     KVNLNTVQRAYKEMGDMNIINTFKNYQSSVTVDENILKNLKLELINESLSVFIEDMKA
                     INVSKEEVLKIIEDKY"
     gene            6511..7227
                     /locus_tag="KR250_003531"
     CDS             6511..7227
                     /locus_tag="KR250_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0069414.1"
                     /translation="MIEIKNVSKTYKRMQGLKIRKIEALKNVSFNIEKGKITALLGIN
                     GVGKSTMLKAIAGLINIDSGEIRIDGEKINEKVYNKLAFVPDVQSHFSNTTIKETFEF
                     MEIFYSKWNKEKSKEMMDIFKLDEDEIIDNLSKGNIARVKLILGFCQDPEYILLDEPF
                     TGIDLFKREEFIGVIAQYMEENQAIIITTHEIVEIESLVDEVVILDEGQIITSFNAEE
                     LREREGKSILDKMREVYKNE"
     gene            7220..>7725
                     /locus_tag="KR250_003532"
     CDS             7220..>7725
                     /locus_tag="KR250_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0069415.1"
                     /translation="MNKLLYLINVDLRRSNKFYIAYISFFSLITLSLNLFEINKFKNS
                     KFIMNLAIEDFGGIFYGLGILSNLGFIQSILFFGILGLFIYYVFMWKREFFSNNQSIY
                     TLMMLPQNKFKICISKSIALITMIYGFLISQTATLFICKYIFNFIFRNMPIINMNFAK
                     DLYYLNLK"
BASE COUNT         3014 a          893 c         1262 g         2556 t
ORIGIN      
        1 tataatttgt gaatttttta ttatagaaat aaatatgtta ttattatgtc aaaatttagc
       61 attagattca tgtagagtat gttttataca tatattataa ttaattcttt gaagattgca
      121 tattcaaaat acacaccatt ttttttaatt ctataatata atatttatat atttaatgtt
      181 aggatgtgat ttaaggtgta ttttacatat attataagat gtaaagatga ttctctatac
      241 actggatata ccagtaatat agttagaaga atgaatgaac ataaattagg aattaattca
      301 aaatacacaa gagcaaaggg atttgaaaaa ctagaagttt attttgtgac caacacaaaa
      361 agcaatgcta tgaagttaga atattatata aaaaaattaa ctagaaataa aaaattgtct
      421 ataataaaaa acccaagtat acttataaat ttaattgata ataaagaaga ttatattatt
      481 ggtaaggaaa tagaacaatt aacataaata tttatagtaa cattataatg ggtgcatatg
      541 gtgtaaaaac ttgacaataa atactattgt aatataatga agttataaat atagtaaatt
      601 aaaatagcta ggggagccaa gagctgagag gtataccgac ccttacacct gatctggata
      661 ataccagcgt aggaaagctt agtaaaaatt caagtatgta ttatttgtga tatatgctat
      721 ttctaagcta tacctattgt aggtatagct tttttaatta agttatttaa attaaacttt
      781 aggaggcaac aatgagtaat tataaaatac caacacttac aatagccggc tctgattcat
      841 ctggaggagc tgggattcag gctgatttga aaacgtttag tgcaattggt acttatggta
      901 tgagtgtaat aacagctata acagcacaga atactaaagg agtttttgct gtagaggatt
      961 taaataagaa aataataaaa aaacaaatag aggcagtctt tgaagatata cctccaagag
     1021 ctgtaaaaat aggaatggtt tcaagtcctg agattatact tgaaatagtt gagaatctaa
     1081 agaaatacaa tccaaaatat ttggttgtgg acccagtaat gatatctaaa agtggatatt
     1141 atctattaaa gccagaggca aaagaaaact taattaagta tttgatacca cttgcataca
     1201 taattacacc aaatatacca gaggcagaag aaataacagg aataaaaata cataatgtag
     1261 atgatatgaa gagagtagga gaagaaatac tacaacttgg acctaaattt gtacttatga
     1321 aagggggtca tttagatgga gaagcagtgg atattttagt tggtaaaaat atatttaaag
     1381 tttataaaag tgaaagaatt gataaaaaaa atacacatgg aacagggtgc acattatctt
     1441 ctgcaataac atcttatcta gcattaggat atgaaataac agaagcagta aatctaagta
     1501 aaatctatat aacagaagca ataaaaagaa gttttgatat aggtcatgga gtaggaccag
     1561 tacatcattt ttataaattt gagtaataaa aaaatattgg aggtagaata tgtataattt
     1621 aattaaagat gtaaaaaaat taaatccatt ggtaattcat tatactaata atgtaactat
     1681 aaatgattgt gctaatgtaa ctttagctgt aggagcaagt cctcttatga gtttttcata
     1741 tgaggaagta gaagaaatgg taagtgtagc aaactctgta gtaataaata taggaactat
     1801 gaactcaaat atgttggatt tattcctatt agctggaaaa gcagcaaata agtacaataa
     1861 acctgttgtt ttggacccag ttggagtatt tgcaagcaaa gcgagagcag aacttacaag
     1921 tagactttta aatgaagtca aatttagtgt tgttaaaggg aatgtatcag aaataaagtt
     1981 tataggtggc tttaatgtaa ggggtaaagg tgtagattca tttgatgaag aagaagattc
     2041 tactgagata ataagaaaga ttgctgaaaa gcttgaatgt gtagttgtag caactggaaa
     2101 aatagatata ataactaatg gtaaaggtac atataaaata aacaatggaa ctgataaatt
     2161 aaaaggaatt acaggaactg gatgtatgac tgctagcttg attgcaagtt ttatggctgt
     2221 tactgagaat atattagaag cagcaacaat gggtgttctt acaatgtctt taagtgggga
     2281 attagctaat ttaaataatc caccaatagg tacatttaaa gaaaatctta tgaatgctat
     2341 ttatcaaatg gatatagata ctttaagtaa aaattctaat attgaatttt taaattaata
     2401 aagagtaagg gagacttaaa atgattgata aagaatcttt aaaaaaatgt ttaaagttgt
     2461 atttagtaac tgactctgaa atgcttaaag gtagagactt ttataaatgt cttgaagatg
     2521 caatatcttc tggaataaca actgtacaac ttagagaaaa aaatgcttct ggaagagagt
     2581 ttttaagaaa agctatgaaa cttagagaaa taactaaaca atatggtgtt aagtttataa
     2641 taaatgatag agtggatata gcacttattt gtgatgcaga tggagtacat gtaggacaga
     2701 gtgacattga tgttagagag gttagaaagc ttataggaaa caataaaatt ctaggagtat
     2761 cagcaagaac tttagaagaa gctatttgtg caaaaaatga tggcgcagac tatttaggtg
     2821 tagggtctat attcacaaca tctactaaat tagatgcaaa atcagcttcg tttgaaactg
     2881 ttaaagaaat taaagaaaaa gtagacatgc catttgtact aattggaggt ataaatttag
     2941 acaatataga taaacttaaa tgtttagaaa gtgacgggta tgctataata tcagctatat
     3001 taaaagctga agatatctct aaagaagtgg aaaagtggac attaaaaata taaaattggc
     3061 tctggtagtg aaaaaaatgg aaaaatttga tataatctaa gagtaaaatt taagcaatta
     3121 taaatgataa acttttctac atatactttt taatcaagat atatgtagat ttggttaaaa
     3181 aggagctata aatgattagg atattgttag tgtgtgttgg aggcatgtct tctaccctat
     3241 tggtaaataa aatggagaag gatgcaaaaa aaagaaatat agactgtaaa atatgggcag
     3301 ttggagaagg agatataaaa tcggagcttg ataactttga tattttactt cttggaccac
     3361 aacttagatt tatgcttgat gacgtaaagt ctatagttgg agatagagct ccagtatcta
     3421 taatagatat ggtaaactat ggcacttgta atggtcatgc tgtgttaaat tcggttttag
     3481 aaatattaaa ataaataata aaagtggatt ctatataata gagtccattt ttattatttg
     3541 ttattaatag gcttaaaact tagcttacca tttctttttt taatctccac atagcaagtg
     3601 aaaaacttga acagcacaat attaaaaata gtaggataac aggcagtata tctccaataa
     3661 gaatagtctc accaaatatt aagctacgta aagcgttaat aacatgggta aatggattga
     3721 gcatcactgc tacttttaat cctccagata aactttctat agggaaaaga gctgaactta
     3781 aaaagaaaat aggaagtaca attgcagtca taattgtttc ataaactatt tcatttggca
     3841 ataataaact tatagaataa gctagacttg ataaaaagaa ggctgtcata aatatcagta
     3901 ctatcataag taggattcca ccaatgccag attctatcct tactgagaag aatatactta
     3961 ctatacacaa aatagttact tctatgaaag aaactaagat agcttctaat aattgaccaa
     4021 ggacgattga atacctactg ataggtgcaa ttaagactct atagaaactt ccactgttct
     4081 tcattataaa gtttataata ccaccactac tacaagaaga aaacacaacc agtacaatga
     4141 tacctggtag tataaaagca gtataattat tgatatttat attattcata gtttggtttg
     4201 caatagaact ataaagcaca agccaaagaa aaggttgtaa tatagtaact acaaatgaga
     4261 ttggattttt aaaacgccac ttcatactac gccataagat atttaacatt cccattcctc
     4321 ctttaagctt tttttagaac ttgtaagtgc taaaaataca tcttcgagac ttggctctac
     4381 aatttctatt gcatcaaatt ctatatcatg ttctaaaagc catttattta ttaaagtaaa
     4441 agcagttcta ctatcgttta cacttataaa aattgaattt tctcgtacgc tcataaactt
     4501 tactaaacca gtacttttaa ttgaatcttt atattttttt atatcttcag tattatgaaa
     4561 agttatacgt agtatatttt gtctaatgta actacgtaaa cttgatggtg ttccttgtgc
     4621 taaatccttt ccattcttca taatacaaat attatcactc aattgttcag cttcttcaag
     4681 ataatgagta gttagaaata tagttgtgcc atactcatct ctaattttta ataacatatc
     4741 ccacatagat tttcttgaat cgacatccat accaacagtt ggttcatcta aaaataggat
     4801 tttaggagat gataccatat tcatagctat gtccaatcta cgttttacac cccctgaata
     4861 tgatgaagta ggatatttta agtaactaga taagtcaaac ttatcaatta aagagtctat
     4921 tctttgtttt gcaatctgtg gttctacttt atacatttta ctttgaaata ccatattttc
     4981 cataagggat aaatgttcat caatagagat ttgctgtgct acacaagcaa tttgagtacg
     5041 tatccaagaa ggattatcta ctaaatcttt tccaaacatt gttacatttc cagatgtagg
     5101 tttgtaaaag gtagtcaaaa tatttataag agaagattta ccagcaccat taggaccaag
     5161 taaagaaaaa atttctccag catttacatt aaagcttaga ccatttaaag ctttcacacc
     5221 atttttatat tcttttacta aattgtctat ttcaatcgct aacatatatt attcctcctc
     5281 tttaagtgga aatattatct ctgtaatata gttatctggg tttcctttta tcaacatacc
     5341 tggtccttta ataaatactt ctctccaagg agtttgtatt tttatattat tttcatgaat
     5401 atatttaaat atcatttcat acgtttttgg caaattacta taagagcctg tgtgagtaaa
     5461 aaataaagct tttgttttag gaaattcttt tgtagttata cccatacgat ttggagtttc
     5521 agcagttgga acacataatt ccatttctgc aacaccagtt ttttggtcaa catcatagta
     5581 gcaaaagaat ggtgcaccgt tagactttcc ttttatagct ttaaatacat ttggaaaata
     5641 ctttgttgcc tcagtcatag gtccattata acgcatggta gccactgtga ctgattcgat
     5701 atttttgatt tcaattttgc aattcatttg tttacctcct aatgtgtgtt catattctta
     5761 ctagattttt aagcattaat acatataaga ttttgattag ttgaaaacat ataatcttat
     5821 ctcgtttgct tgttttcaag tctaacataa cataaaatat atatcctctc atttgatgct
     5881 ccattttgta aagttattat ttaagcttta ctttagattt tttagcaaat taaaataaag
     5941 caatttataa aataagtatt tacaaattaa gtgtattaat cgtataatac acttaatagg
     6001 atactagata aagtctgaaa taaaggcgta tttttttaaa gtaagtgtac tatcgtatta
     6061 atacactaga ataggaggaa tcatgaattt tgaattagat aatacaactc ccatttattt
     6121 gcaaatagta aagtatataa agagacaaat agtaacagga gagttgaaac caggagagac
     6181 aataccatca agaagagaaa tggcacttaa tctaaaagta aacttaaata cagttcaaag
     6241 agcatataag gagatgggtg atatgaatat aataaatact tttaaaaatt atcaaagcag
     6301 tgtaacagtt gatgaaaata tattaaaaaa tttaaaatta gagcttataa atgaatcact
     6361 atctgttttt atagaagata tgaaagcaat aaatgttagc aaagaagaag ttttaaaaat
     6421 aatagaagat aagtattagt tttttaacaa atagaaaact tagtttagaa agtttattta
     6481 taagcatatt ggatgtggag gatagtaaat atgatagaaa taaagaatgt cagcaaaaca
     6541 tataaaagga tgcaagggct taaaataaga aagatagaag ctttaaaaaa tgtaagtttt
     6601 aatatagaaa agggaaaaat aactgcatta cttggtataa atggtgttgg taaatcaact
     6661 atgttaaaag caatagcagg acttataaac attgatagtg gagaaattcg tatagatgga
     6721 gaaaaaataa atgaaaaagt atacaataaa ttggcttttg ttccagatgt acaatctcac
     6781 tttagcaata caacaataaa agaaacattt gaatttatgg aaatttttta ttcaaagtgg
     6841 aataaagaaa aatcaaaaga aatgatggac atatttaaat tggatgaaga tgaaataatt
     6901 gataacttgt caaaaggaaa cattgcaaga gtaaaattaa tacttggatt ttgccaagac
     6961 ccagagtaca tacttcttga tgaaccattt acaggaattg atttgtttaa gagggaagag
     7021 tttataggag taatagcgca atatatggag gaaaatcaag ccattatcat aactactcat
     7081 gagatagtag agatagaaag tttagttgat gaggttgtaa ttcttgatga agggcagatt
     7141 ataacttcat ttaatgcaga agaattaaga gaaagagaag gtaaatctat attagataag
     7201 atgagggagg tatataagaa tgaataaact tctatatcta ataaatgtag atttgagaag
     7261 aagcaataag ttttatatag cttatatatc atttttttca ttaattacac ttagtttaaa
     7321 tttattcgaa attaataaat ttaaaaattc taaatttatt atgaacttag caatagaaga
     7381 ttttggaggt atattttatg gactagggat acttagtaat cttggtttta tccaaagtat
     7441 attgttcttt gggatattgg gactttttat atactatgtt tttatgtgga aaagagagtt
     7501 cttttcaaat aatcaaagta tttatacact tatgatgtta cctcaaaata agtttaagat
     7561 ttgtatatct aaatcaatag ccttgataac aatgatatat ggttttttga taagtcagac
     7621 agcaacatta tttatatgta aatatatatt taatttcatt tttagaaata tgcctataat
     7681 caatatgaac tttgccaaag atttatatta tttaaacttg aagag
//
LOCUS       DAEPIN010000122         7649 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.124, whole genome shotgun sequence.
ACCESSION   DAEPIN010000122 DAEPIN010000000
VERSION     DAEPIN010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7649
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.124"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            complement(<1..493)
                     /locus_tag="KR250_003533"
     CDS             complement(<1..493)
                     /locus_tag="KR250_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069416.1"
                     /translation="MNKVYIVTTYTGTILSYLIRNISKKLYTHVSISLNENLKPMYSF
                     GRLNPRNPFIGGFVEENINQGLYAIRKNTVCRVYSLEVDNLQYENLYKNIKLISDYRE
                     DYYYDTMALIYLLMNIQRQSEYKYVCSNFVADMLEKSEIDILNKQAFEVTPNDFYNLS
                     GLTL"
     gene            complement(652..1365)
                     /locus_tag="KR250_003534"
     CDS             complement(652..1365)
                     /locus_tag="KR250_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2140 domain-containing protein"
                     /protein_id="HBH0069417.1"
                     /translation="MGKVAKILLSLIVIVLVLVGIVVYILTPKERYDVSSQNNKIIDE
                     EYLSKGSYINSMEVVKNPLRMVGKISVSEEEFKNLIYTLMQKHGINELENNFVEMKNG
                     KIKVAGPYKVFGLVNSQYELELRPTLKDGNLVVSLENVKIGKFKLSDKMLEKILSNYN
                     KKVPFEVNGNKITLEKSYLYPITLKNISIKKGNVDLDMEVEIDLKSQINGALGYLKDS
                     GKVQDILNHLINLKKTSVG"
     gene            1657..3321
                     /locus_tag="KR250_003535"
     CDS             1657..3321
                     /locus_tag="KR250_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HBH0069418.1"
                     /translation="MSNETNSSNFIKNIIINDLETGKHDSIITRFPPEPNGYLHIGHA
                     KSICLNFGLAKEFNGKANLRFDDTNPLKEDVEYVESIKEDVKWLGFDWNELNFASNYF
                     DEMYKRALILIKKGKAYVCDLTQEEMREYRGTLTEPGKESPHRNRTIEENLDLFERMK
                     NGEFKDGEKTLRAKIDMSSPNINLRDPIIYRISHSTHHNTGDKWCIYPMYAFAHPIED
                     AIEGITHSICTLEFEDQRPLYDWFVKECEMENIPRQIEFARLNINNTVMSKRKLKQLV
                     DEGIVDGWDDPRVPTISGIRRKGYTAEALRNFCSEIGVSKVNSTVDSQMLDYFLRENL
                     QPKAPLTMGVLRPLKLIITNYPEDKIEMLEIENNAKDESQGKRLVPFSRELYIEQDDF
                     MEEPVKKYFRFFPGNEVRLKGAYFVKCTDVIKDENGNVVEIHGTYDPETKSGSGFTGR
                     KVKSTIHWVDAKSAIPCEFRLFEPLILDDIPENEGKHFLEQINPNSLEILQGFVEPTQ
                     IKDAKPFDKFQFVRNGFFSIDNKYTTDEKFVFNRIVPLKSSFKPGK"
     gene            complement(3428..4327)
                     /locus_tag="KR250_003536"
     CDS             complement(3428..4327)
                     /locus_tag="KR250_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HBH0069419.1"
                     /translation="MKKVKLIYNPNSGEKKILSNLDTIIELYQEKNYLLIPYRLTIKE
                     PVKNAFKEVDSSYEHILIAGGDGTVDLVVNAMKELDIKIPIGILPTGTANDFSNALQI
                     SFDIKEAINEIINSKPKKIDIGKVNNKYFINVASAGMFTDVSQRINTDLKNSFGRISY
                     YIKGIEEALYMRKFKIKVSSEEMYYDGDMYLMLIFNGKTAGNINLAYKAEIDDGYLDV
                     IIFKGMPIPKSIPVLISVLRGDYLDQYNGNEILYFKTKKVHIECEDSLITDIDGEKGP
                     DFPLNIECIKDGIEVMGICSEKS"
     gene            complement(4354..4992)
                     /locus_tag="KR250_003537"
     CDS             complement(4354..4992)
                     /locus_tag="KR250_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003356606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HBH0069420.1"
                     /translation="MDQNLNWLNEKRIEKTIKSLENNNMKGYLVSNKEELLKKIEEIV
                     KAGSLVGCGGSMTLFETGVIDFIREKYNFLDRYAKDIKPEEMKEIYRKSFSADAYFTS
                     TNAITEDGELYNLDGNGNRVAAMIYGPDKVIVIAGVNKIVKDVDEAIKRTKTIAAPAN
                     CKRLSTNTGCKTTGICIDCSSPGRICCSYTLIKRQLTPERIHVIFLNEDFGY"
     gene            complement(5329..6327)
                     /locus_tag="KR250_003538"
     CDS             complement(5329..6327)
                     /locus_tag="KR250_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4003 domain-containing protein"
                     /protein_id="HBH0069421.1"
                     /translation="MEQSFINGKVNLLIENYKALNEVKGSWQMGLIQHSCALAFTLKN
                     KKISPRLVEERIELIKKNTGLFSNFRGYNMFYMATLLSFESNPESSFKMILDIYKELK
                     SEKFWGDTYLPLTASIVYENREKMDYLTCISKMKIIYDYMRKKHPFLTSSDDYCNIAL
                     IAIHSKNLDEDLEYIEKCYEFLNENGFYKGNDLQALSQILLFSDDRTMLKCKKTIELK
                     KAFKENDCKMNYYGYPIIGAISLLDYREDEIIEEIKNVSNKLKEEKGFGNWSLGKSNR
                     VMISSAIVASIYADLIRNENNIGSITNNIFLNIVVAIQIACVMAATSAAIASSSSN"
     gene            complement(6425..7000)
                     /locus_tag="KR250_003539"
     CDS             complement(6425..7000)
                     /locus_tag="KR250_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069422.1"
                     /translation="MKLKEALITCVVCSVCLTPLLVNGETKIDADHCKESIVNIDTIV
                     KSGKSFNSKYNQGMEDYKNSDEYKLKVQKEKLQNQLGIKIKKLVPITFEVSYYTSLNQ
                     ENGYGAITATGDDLEDGFVANNHLSFGTKIIVDGKLKTVMDRGSSKYFSNSNAIDVFV
                     PKWSNESESEYYDRVNNMGRHYKKGYIIVEE"
     gene            7316..7579
                     /locus_tag="KR250_003540"
     CDS             7316..7579
                     /locus_tag="KR250_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069423.1"
                     /translation="MIKNTKQITVEVNEILLQEMSKITNYENNILTDLINESIVNYIL
                     ENKKHNIENKDLEEVIKFYKKENTEKPKSAARAIAEAKTHTLY"
BASE COUNT         2658 a         1170 c          828 g         2993 t
ORIGIN      
        1 ctaaggttaa gccagataaa ttataaaaat catttggcgt tacttcaaat gcttgtttat
       61 ttaaaatgtc tatctcactt ttttctaaca tgtcagcaac aaaatttgaa catacatatt
      121 tatattcaga ttgtctctga atattcatta gtaaatatat taatgccatt gtatcgtaat
      181 agtaatcttc tctataatca ctaattaatt ttatattttt atatagattt tcatattgta
      241 aattatctac ttctaacgaa tataccctac acacagtgtt ttttcttata gcatataatc
      301 cttgatttat attttcttca acaaatcctc caataaatgg attccttgga tttagccttc
      361 caaaagaata cattggtttt agattttcat ttaatgatat tgacacatgt gtatacagct
      421 ttttacttat attccttatt aagtaagata atatagttcc tgtatatgtt gttacaatat
      481 agactttatt catcatttcc ccccaatcta gtaactaaat aatattatac acttacaatt
      541 taatatcata agatattcta acataataaa tgtggtttta caaattttga ttttaaggta
      601 ctttaatatt atataatata ataaaaatat aaatataagt tttctatatt tttaaccaac
      661 agatgttttc ttcaaattaa tcagatgatt tagtatatct tgaacctttc cagagtcctt
      721 taaatatcct aatgccccat ttatttgtga ttttaaatct atttctactt ccatatccaa
      781 atcaacattt ccttttttta tagatatatt tttaagtgtt attggataaa gataactttt
      841 ttccaaagta atcttatttc cattaacctc aaatggaact tttttattgt agttacttaa
      901 tattttttct agcattttat cacttagttt aaattttcca attttcacat tctcaagact
      961 tacaactaag tttccatcct ttaaagtagg tctcaattca agttcatatt gactatttac
     1021 caaaccaaat accttatatg gtcctgctac ctttatttta ccatttttca tttcaacaaa
     1081 attattttca agttcattaa ttccatgctt ctgcatcaat gtatatatta aatttttaaa
     1141 ctcttcctct gaaacagata tttttccaac cattctaagt ggatttttta ctacctccat
     1201 agaatttata tatgagcctt ttgataaata ttcttcatct atgattttat tattttgtga
     1261 actaacatca tatctttctt ttggtgtcaa gatatataca acaattccta caagtactaa
     1321 aacaataaca attaaactta acaaaatctt tgcaaccttt cccataatcg tctcctttac
     1381 aaatctgtca tcagatttaa aataaattta gataactaac ttcacataat attcttaaca
     1441 atatacatac tataatacag tcttttaaaa attaattcaa ccaatctaat gttttgtaaa
     1501 taaactgtaa aaacatgttt ttagtataaa agttaatatt ttgcataata ctcttaatta
     1561 cacctttatt tttttctaat cttggattaa aattaatatt atgcaataca gtgtagtgtt
     1621 ctgtattata atcaaattaa ataggaggta tagaatatgt caaatgaaac gaactcatct
     1681 aactttataa agaatattat tataaacgac ttagaaacag gaaaacatga cagtatcata
     1741 actcgttttc cacctgagcc aaacggatat ttacatattg gtcatgcaaa aagtatatgt
     1801 cttaattttg gattagctaa ggagtttaat ggaaaagcta atttaagatt tgatgataca
     1861 aacccattaa aagaggatgt tgaatatgta gagtccataa aagaagatgt aaagtggtta
     1921 ggatttgatt ggaacgagtt aaattttgca tctaactact ttgatgaaat gtataagaga
     1981 gctttaatac taataaaaaa gggtaaagct tatgtatgtg atttgacaca agaggaaatg
     2041 agagaatatc gtggtactct aactgaacca ggaaaagaaa gtcctcatag aaacagaact
     2101 attgaagaaa accttgattt atttgaaaga atgaaaaatg gtgaatttaa agatggtgaa
     2161 aaaacattaa gagccaaaat agatatgtct tctccaaata taaaccttag agacccaatt
     2221 atatatagaa tatctcattc aacacaccat aatacaggag ataaatggtg catatatcct
     2281 atgtatgctt ttgctcatcc aattgaagat gcaattgaag gaataactca ctctatatgt
     2341 actttagagt ttgaagacca aagacctctt tatgattggt ttgttaaaga atgtgagatg
     2401 gaaaatatac ctagacaaat agaatttgct agacttaata taaataatac tgtcatgagt
     2461 aaaaggaaat taaaacaact tgtagatgag ggtattgttg atggttggga tgaccctcgt
     2521 gttcctacaa tatccggaat cagacgtaag ggatatactg cagaagcatt gcgcaatttc
     2581 tgtagtgaaa taggggtatc aaaagttaac tctacggtag atagtcaaat gcttgattat
     2641 ttcctaagag aaaacttaca accaaaggct cctcttacta tgggagtttt aagaccttta
     2701 aaacttatta taactaatta cccagaagat aagattgaga tgcttgaaat agagaataat
     2761 gctaaagatg aatctcaagg aaagagatta gttcctttct ctagagaatt gtatatagaa
     2821 caagatgatt ttatggaaga acctgttaag aaatatttta gatttttccc aggaaatgaa
     2881 gtccgtttaa aaggtgctta ttttgtaaaa tgtactgatg taattaagga tgaaaatgga
     2941 aatgtagttg aaatacatgg tacttatgac cctgaaacta agagtggctc tggctttaca
     3001 ggacgtaagg taaaatctac aatacattgg gttgatgcta aatcagctat cccatgtgaa
     3061 tttagattat ttgaaccatt aattcttgat gatatacctg aaaatgaagg taaacatttc
     3121 ttagaacaaa taaatcctaa ttcattggaa atattacaag gttttgttga acctacacag
     3181 ataaaagatg ctaaaccttt tgataaattc caatttgtta gaaatggatt ctttagtata
     3241 gataataaat atacaactga tgaaaaattt gtatttaata gaatagttcc tctaaaaagt
     3301 tcttttaaac caggtaaata atcttacaat caaaaaatga agacttaaaa tataaattac
     3361 ttctactata aataaactta gttagaagta attttttatt tgagtcttca tcatttactt
     3421 taaatgatta acttttttca ctacagattc ccatgacttc tattccatct tttatgcatt
     3481 ctatgttaag aggaaaatct ggtccttttt ctccgtcaat atctgttata agtgaatctt
     3541 cacattctat atgcaccttt ttagttttaa agtatagtat ttcatttcca ttatattgat
     3601 ctaaataatc acctctaagc acacttatca aaactggtat tgattttggt attggcattc
     3661 ctttaaaaat aattacatcc aaatatccat catctatttc agccttatat gctagattta
     3721 tatttcctgc tgttttgcca ttaaaaatca acataagata catatctcca tcataataca
     3781 tttcctcaga agatactttt atcttaaatt tcctcatata caatgcttct tctattccct
     3841 ttatataata agaaattctt ccaaatgaat tttttaagtc tgtgtttatc ctttgtgata
     3901 catccgtaaa cataccagca cttgcaacat ttataaagta cttattattg actttcccta
     3961 tgtctatttt ctttggtttt gaatttatga tttcatttat agcttctttt atatcaaatg
     4021 aaatttgaag agcatttgaa aaatcatttg cagttcctgt aggcaaaatt ccaattggaa
     4081 tttttatgtc taattctttc attgcattta ctactaaatc tacagtacca tcaccaccag
     4141 ctatcaatat gtgttcataa gaactatcaa cttccttaaa tgcattcttt acaggttctt
     4201 taatagttaa tctatagggt atcaataaat aatttttttc ttgatataat tctatgatag
     4261 tgtctaaatt tgataatatc tttttttcac ctgaattagg attgtatata agttttactt
     4321 ttttcattat ttctccttac tcattttatt cttttagtat ccaaaatctt catttaaaaa
     4381 aatcacatgt attctttctg gtgttaattg tctttttata agtgtataac tacagcatat
     4441 tcttcctgga cttgaacagt ctatacatat accagtagtc ttacatccag tatttgtact
     4501 taatctttta caattggctg gagcagctat tgtttttgtt ctttttatag cttcatctac
     4561 atctttaact attttattta ctcctgctat aactattact ttatcaggtc catatatcat
     4621 agcagcaact ctattcccat tcccatctaa attgtaaagt tctccatcct ctgttatcgc
     4681 attagtactt gtaaaataag catctgcaga aaatgacttt ctatatattt ctttcatttc
     4741 ttctggtttg atatcttttg catatctatc aagaaaatta tatttttctc ttataaaatc
     4801 aatcactcct gtttcaaata aggtcataga acctccacat cctactagag aacctgcttt
     4861 aactatttct tcaatttttt taagaagctc ttctttgtta cttactaaat accctttcat
     4921 attattgttt tccaaagatt ttattgtttt ttctattctt ttttcattaa gccaatttaa
     4981 attttgatcc attttaactt cccccttatt tttaatatgt acttaattat ttataaactt
     5041 tattttcttt ataagtttat tttagtacat ctaaaaaaaa atacaatata aaacttaaat
     5101 agttttacat aattttttcc aaaataactt actcaatcaa aaaaggaaag tagatgtact
     5161 attctacttt ccctatattg tatgtagtat ttattgccta aaatctatat attaattata
     5221 tgtttcatat tatataatat cacagttaat taaaatgtaa tactatcttc tttttaaaca
     5281 tataaattgt attattaaat ttaatatatc caacttcaat aaatcttatt aatttgatga
     5341 agaagatgct atagctgcac ttgttgctgc cattacacat gcaatttgaa tagcaacaac
     5401 aatatttaaa aatatattat tagttataga gcctatatta ttctcatttc gtatcaaatc
     5461 tgcataaatt gaagctacaa tagcagaact aatcattact ctattacttt ttcctaaaga
     5521 ccaatttcca aagccttttt cttctttaag tttatttgaa acatttttta tctcttctat
     5581 tatttcatct tctctataat ctaataatga aattgctcct attattggat atccataata
     5641 attcatttta caatcatttt ctttaaatgc ttttttaagt tcaatagtct ttttacattt
     5701 aagcatagtt ctatcatcac taaataaaag aatttgggat aatgcttgta aatcatttcc
     5761 tttataaaaa ccattttcat ttagaaattc ataacatttt tctatgtatt ctaaatcttc
     5821 atctaaattt tttgaatgaa ttgcaataag tgctatatta caatagtcat cactagaagt
     5881 tagaaatgga tgttttttcc tcatgtaatc atatattatt ttcatttttg atatacatgt
     5941 aagataatcc attttctctc tattttcata aactattgat gctgtaagtg gtaaatatgt
     6001 atcgccccaa aacttttcac ttttaagttc tttgtatata tctaaaatca tcttaaaact
     6061 actttctgga tttgattcaa acgaaagtag tgttgccata taaaacatat tatatcctct
     6121 aaaattagag aataatccag tatttttttt aataagctct attcgttctt ccactaatct
     6181 aggacttatt ttcttgttct taagggtaaa tgcaagtgca caactatgtt gtatcaatcc
     6241 catttgccaa gaacctttaa cttcatttaa tgctttgtaa ttttctatta ataagtttac
     6301 ttttccattt ataaacgatt gttccatatt gccctcctag taatatatct cattgtacta
     6361 atagttttat atttattata tatatattaa atactacaat caatacatat tacttcaaag
     6421 aacatcattc ttcaacaata atgtagcctt ttttgtaatg tcttcccata ttattgactc
     6481 tatcatagta ctctgattca ctttcattac tccatttggg cacaaataca tctattgcat
     6541 tagaattact aaaatactta ctagaccccc tatccataac agtttttaat tttccatcta
     6601 caataatctt agttccaaaa cttaagtgat tgtttgctac aaaaccatct tctaaatcat
     6661 ctcctgtagc agttatcgct ccatatccat tttcttgatt aagacttgtg taataactaa
     6721 cttcaaaagt tataggtact aacttcttta ttttaatacc taactgattt tgtagtttct
     6781 ccttttgtac tttcaactta tactcatcac tatttttata atcttccata ccttgattat
     6841 acttggaatt aaagctttta cctgacttta ctatagtatc tatatttact atagattctt
     6901 tacaatgatc tgcatctatt tttgtttctc catttaccaa taatggagtt aaacatacac
     6961 tacatacaac acatgtaatc aatgcttctt taagcttcat aaattccccc cttattattt
     7021 tgatatatta gcatatttaa tattatatgt ctacatttag atttaaatac cacacccatt
     7081 tgctttaaga agttgtctta tatttaattc accttaaaca cttataaatc ctctatctta
     7141 gaatcatata gaataaagct tttatcatta aatatttttt attaatcttt taaaatctaa
     7201 aactacttta tctaattttc tcttttttag aatttaagaa ttaaaattct ataattttaa
     7261 aacatattga atttattttt ttgagatgat ataattttct taagaggtgg ttgatatgat
     7321 aaaaaataca aaacaaatta cagtagaagt taatgaaatt ttattacaag aaatgtctaa
     7381 gataactaat tacgaaaata atattttgac agatttgata aatgaatcaa ttgtaaatta
     7441 tattcttgaa aacaaaaaac ataatattga aaataaagat ttagaagaag tgattaaatt
     7501 ttataaaaaa gaaaatactg aaaaacctaa aagtgcagca agagctatag ctgaagcaaa
     7561 aactcatact ctgtattaat caaataaaaa agttcctaaa aactaggaac ttttttatat
     7621 taataattat taatatatct caatattta
//
LOCUS       DAEPIN010000123         7432 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.125, whole genome shotgun sequence.
ACCESSION   DAEPIN010000123 DAEPIN010000000
VERSION     DAEPIN010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7432
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.125"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            complement(909..1721)
                     /locus_tag="KR250_003541"
     CDS             complement(909..1721)
                     /locus_tag="KR250_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBH0069424.1"
                     /translation="MYLERPELYKQSEINFWDDEYISKQLLKAHLDTNFEGASRNFNF
                     IEDSVNWIVTVANPANYPKLLDLGCGPGLYAEKFAQKGYKVTGIDFSKRSINYAQNRN
                     KETNLNINYLFQSYLNMNYNEEFDLATLIYCDYGALSTENRRLLMEKIYDSLKPGGKL
                     ILDVFTINKYNNFEEIKYWEINEDGGFWSNEKYMCLQDNCKYNDYNTLEQTLVITEKD
                     ENVFYVWNHYFSKESFLLEVENIGFKSVEFFSNVKGEAYSDDSMSMGLVLQK"
     gene            complement(2091..2993)
                     /locus_tag="KR250_003542"
     CDS             complement(2091..2993)
                     /locus_tag="KR250_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBH0069425.1"
                     /translation="MYNKIKYIPINCEIACNKLKRSMPYKWDLNIYRGCEHGCKYCYA
                     IYSHKYINSNNYFEEIYVKTNIVEKLERQLKSSKWKKEVINIGGVTDSYQPIEADYKI
                     MPEILNLLIKYKTPAIISTKSDLILRDYDLIDKLSRITYINVASTITTVNEDTQRLIE
                     PNGVDSMRRFKMLKEFRKTNASVGLHIMPIIPYITDDFDNINSLFRHAKECNVHYVLP
                     GTLYLRGITRGVFFDFVKKEFPELFDKLSILYSAGSLNREYKNQLYKMVNELRYKYSL
                     SSSYSKIMKEKLKNSGDVQISFFD"
     gene            complement(3043..3504)
                     /locus_tag="KR250_003543"
     CDS             complement(3043..3504)
                     /locus_tag="KR250_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine dimer DNA glycosylase/endonuclease V"
                     /protein_id="HBH0069426.1"
                     /translation="MRLWHKDLIDVLPKNQLVSQWRELLAIKGSIDKKGTPNHLLVNK
                     VLNYSIDEFKFYTKIVHDEMLKRNYKPNELKYTSILKWKNRNFANDISNEHSLNLENL
                     YDDWHNKMYLKQCLYNLEEKATCGGIPINEWNILLCKYSKDYELWSGNIMF"
     gene            complement(3556..4011)
                     /gene="def"
                     /locus_tag="KR250_003544"
     CDS             complement(3556..4011)
                     /gene="def"
                     /locus_tag="KR250_003544"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HBH0069427.1"
                     /translation="MAIRKIRTFDDEILRKKSKYVENVDNKIREILNDMAETMYNTPN
                     GGGLAACQVGVLKRLVVIDLGEGLIKLVNPEIIEQEGEQIVVEGCLSFPEVWGKLKRP
                     KKVTVQALNEYGEKIEIKGSGFMAKCLCHEIDHLNGIVFTDKIIEHVKL"
     gene            complement(3983..4246)
                     /locus_tag="KR250_003545"
     CDS             complement(3983..4246)
                     /locus_tag="KR250_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0069428.1"
                     /translation="MKNKIKILREKLGLTQEQLGRLVGTSRQAINAIETGKNEPSIWL
                     AYDISRIFNEPIESVFLFEESERKSRAQISRGEYYGYKKDKNI"
     gene            complement(4343..5059)
                     /locus_tag="KR250_003546"
     CDS             complement(4343..5059)
                     /locus_tag="KR250_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0069429.1"
                     /translation="MIKIINAFKQEFEQIKRDAMLFIVCVSPILCGVFIKFGIPLIQN
                     ISLNKFYYQLNLEPYFLMFDLLLAFITPFMFFFASTMVILGEIDDSISRYLIVTPLGK
                     TGYLISRFGIPGILAFIITMILLIFFSLTKISLLLNLSISLLVLLQGVIISILVISLS
                     SNKLEGMVITKFSGVFMMGILAPFFILNKAQYILFFLPTFWISKAFKEDNYVYMFISI
                     VISLIWILLLFKKFSKKIVK"
     gene            complement(5056..5739)
                     /locus_tag="KR250_003547"
     CDS             complement(5056..5739)
                     /locus_tag="KR250_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0069430.1"
                     /translation="MRLKNLILGDIKFQFKYGFYFLYLFLSIIYICIINVFPTFMREK
                     IAIIMIYSDPAAMGLFFMGAIVLLEKSQRVLNSLAVSPVKVSEYILSKVISLGVISSI
                     VAMFIAITLNLDNIIISTIGTFFSSIIFSLLGLIIASKASSLNQFIVLSIPIEIICFI
                     PPILNVLLDTKSYANLYPFNICISLISGDKNFIMINILILISIIIIIYFITYYFICSS
                     WKKVGGVKL"
     gene            complement(5736..6581)
                     /locus_tag="KR250_003548"
     CDS             complement(5736..6581)
                     /locus_tag="KR250_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0069431.1"
                     /translation="MIKVDDLSFSYTDRDFLQNINFEVGKGEILGFLGPSGAGKSTLQ
                     KILIGMITNYGGSVIVNGVESKRHSNKFYENIGVDFEFPSLYEKLTAIENLKYFGSLY
                     SKKLLSIDELLKSVGLENESNKRVSEYSKGMKSRLNFIKALLHNPDILFLDEPTSGLD
                     PSNSKVMKDIILSEKSKGKTIILTTHNMLDATELCDRVAFIVNGKISALDTPHNLIMS
                     KGAIKVRYTYFDNGEKTSECFLNNTANDKNLNMLIEKNKLLSIHSSEPTLNDIFIEIT
                     GRNLQ"
     gene            complement(6695..7285)
                     /locus_tag="KR250_003549"
     CDS             complement(6695..7285)
                     /locus_tag="KR250_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBH0069432.1"
                     /translation="MPKSYSDKEREYIIKRLKEEARLCMEQYGIRKTTVDELVKRVKI
                     PKGTFYLFFQSKELLFFEVLRDIHDSIQKEILYEINRVDESITCEQLTDIFMKFYRMV
                     DSTSILNLMINGEFEILVRKLPDSIIEEYFRHDDFEIGEIISCIPNAKNKDIESFSGA
                     FRAVFLTMLYKREVGSNCFEDALRLMINGLVIQLME"
BASE COUNT         2622 a         1172 c          680 g         2958 t
ORIGIN      
        1 catagggttc ctgcttgata gaattttgca tagtcaattt actaaatact tgtctaattc
       61 tttttttatc attttaaata tacattttaa taatattagt aagctatact tccttaagca
      121 tatattttcc ctaaagtaaa actattgatg attattccat tatataacct aattataaat
      181 tcagtaagtt catcacatac tataccctta attctcaatt ataaagtttc ctaaaaaata
      241 cttgaaaatt taactatgaa aaaatttcct attgcctaat atgtataagt aaggagtact
      301 atatagtact ccttatattg tactcttata acattgatgc cttaatatta ttttaatctt
      361 aatcattatt gatttgattt aaaattataa acaagttcca atattttaat tatgtcaact
      421 atatctctta tagttttaat aatctcatca attggtttat gtgcttgttg cactttatca
      481 atagtattaa ttaaatccat ttacacatct agcacctgta tggcaattta gcattatcct
      541 tatcttatac tttgttgtaa attcttgatt acataattct attatctaac ttatagctgt
      601 gcaatctaca ttatctgtaa atactttgct ttatatattt tccctgaacc tctaacatta
      661 atataacagc ctttttgcta tacactttta actacatact taattttaat aacattactt
      721 gtttataaaa atatactata gataaatttc acataaaaat aataattcaa gtattgattt
      781 ttatataagc aaatttgtaa tgtactaaaa ataacataaa agtttccatt tttcaatatg
      841 taagctttta ttatgttatt ttattgttaa aggtttcaag tttttattta ttcattttaa
      901 aaaaattatt atttttgtaa aactaatccc atactcatac tatcgtcaga atatgcttca
      961 cccttaacat tactaaaaaa ctcaacagac ttaaatccaa tattttcaac ttctaataag
     1021 aaactttctt ttgaaaaata gtgattccaa acataaaata cattttcatc tttttcggtt
     1081 attacaagag tttgttctaa tgtattataa tcattgtatt tacaattatc ttgtagacac
     1141 atatattttt cattactcca aaatcctcca tcttcattta tttcccaata tttaatttcc
     1201 tcaaaattat tgtatttatt tattgtaaat acatctaata ttaattttcc tccaggtttt
     1261 aaactatcat aaattttttc cattaataac cttctatttt cagttgacaa agctccataa
     1321 tcacaataaa ttaaagtagc caaatcaaat tcctcattat agttcatatt taaataactt
     1381 tggaacaagt aatttatgtt taaattagtc tccttatttc tattttgtgc atagttaata
     1441 gaacgttttg agaaatcaat tccagttacc ttataacctt tttgtgcaaa tttctctgca
     1501 tataaacctg gaccacaacc taaatcaagt aatttaggat aattagctgg atttgcaact
     1561 gtaactatcc aatttacaga atcctcgata aagttaaaat ttctacttgc tccttcaaaa
     1621 ttagtgtcta agtgtgcttt taataattgc tttgaaatat actcatcatc ccaaaaatta
     1681 atttcacttt gtttataaag ctctggtctt tctaaataca tacataattt gtttaacaaa
     1741 acataccact ccttaaaatt tatttttaaa ataaaaaaac cacagagttt actctgtggt
     1801 ttaacttcaa aaagatgagt ttaaataaga gtaaaataga accaaatatt tttaagcaca
     1861 acaataaaac tatagcttta ctttgtttgc ttattaaatt tgattttatt cactcttttc
     1921 cagcaatact tatatcaacg attttatgat atcaaatatc accgaacaac atcatccttt
     1981 catattttta ttttatgaat taattatata tttaaatttt atatttgtca ataaatttta
     2041 attttaataa attttaatca tcatagtaaa aaaaattttt ttatataaaa ctaatcaaaa
     2101 aaagatatct gtacatctcc tgagtttttt aacttttcct tcattatttt agagtaactg
     2161 cttgatagag aatacttata tctaagttca ttaaccattt tatacaattg atttttatac
     2221 tccctattaa gtgaacctgc actatatagt attgataatt tatcaaataa ttcaggaaac
     2281 tcttttttaa caaagtcaaa aaaaacacct ctagttatac cccttaagta taaagtccca
     2341 ggtaaaacat aatgtacatt acattctttt gcatgtctaa atagcgaatt tatattatca
     2401 aaatcatctg taatatatgg tataattggc ataatatgta gaccaactga tgcgttagtt
     2461 tttctaaatt cttttaacat tttaaatcgt ctcattgaat ccacaccatt tggctcaata
     2521 agcctttgtg tatcttcatt tactgtggta attgtagatg ctacattgat atatgtaatc
     2581 ctagatagct tatcaattaa atcgtaatct cttaaaatta aatctgactt tgtagaaata
     2641 atagctggtg tcttgtattt tattagcagg tttaatattt ctggcataat tttatagtca
     2701 gcttctattg gttgataact atctgtaact cctcctatat tgattacttc tttcttccat
     2761 ttactgcttt taagttgtct ctcaagtttt tctactatat ttgtttttac ataaatctct
     2821 tcaaaataat tattggagtt gatgtactta tgagagtaaa tagcataaca atatttacat
     2881 ccatgctcac atccacgata aatatttaaa tcccatttat atggcatact cctttttaat
     2941 ttattacaag ctatttcaca atttattgga atatatttta ttttattata caaataatca
     3001 cctctttata tacaataata ctataatgtt aatctatagt aattaaaaca tgatatttcc
     3061 agaccacaat tcataatctt tactatattt acaaagtaat atattccatt catttattgg
     3121 tattcctcca catgttgctt tttcttctaa attatataag cattgcttta aatacatttt
     3181 attatgccaa tcatcatata aattttctaa atttaaacta tgttcattag atatatcatt
     3241 tgcaaaattt ctatttttcc attttaatat acttgtatat ttaagttcat taggtttata
     3301 atttcttttt aacatttcat catgtacaat ttttgtataa aatttaaatt catctatact
     3361 ataattcaat actttattca ctaatagatg gtttggagta ccttttttat ctatagaacc
     3421 ttttatagca agtagctctc tccattgact tactaattga tttttaggta atacatcaat
     3481 taaatcctta tgccataatc tcataattta actccttact ataaaatatt ttataaattc
     3541 aataaccaat ctatcttaca attttacatg ttcaataatt ttatctgtaa aaactatacc
     3601 atttaaatgg tctatctcat gacaaagaca ttttgccata aaacctgaac ctttaatttc
     3661 tattttctca ccgtattcgt ttagagcttg cactgtaact ttttttggtc tttttaattt
     3721 accccatact tcaggaaaac ttaaacatcc ttcaacaaca atttgttccc cttcttgttc
     3781 aataatttca ggattaacaa gcttaataag tccttctcct aaatctataa caactaatcg
     3841 ctttaatact cctacttggc atgcagctaa tcctcctcca tttggagtat tgtacatggt
     3901 ctctgccatg tcatttaata tttctcttat cttattatct acgttttcta catacttact
     3961 cttttttctt aaaatttcat catcaaatgt tcttatcttt cttatagcca taatattctc
     4021 ccctacttat ctgtgctctt gattttcttt cactttcttc aaataaaaat acactttcaa
     4081 ttggttcatt aaaaattctt gaaatatcat atgccaacca tattgaaggt tcatttttac
     4141 cagtttctat agcatttata gcctgtctag atgttccaac taatctccct agttgttctt
     4201 gtgtcaatcc taatttttct cttaatatct ttattttatt tttcatattt gctccattca
     4261 tgtaaggtta actttacatc ttaaatgtaa catttttcaa cattactgtc aagtcaacct
     4321 tacatttata tattaaattt tcttatttaa ctattttttt actaaacttt ttaaataata
     4381 ataaaatcca tattaatgat ataacaattg aaataaacat atagacataa ttatcttcct
     4441 taaatgcctt cgatatccaa aaagttggta agaagaacag aatatattgt gctttattta
     4501 aaataaaaaa tggtgctaaa atacccatca taaaaacacc tgagaatttc gtaattacca
     4561 ttccttctag tttatttgat gataaagaaa tcactaatat tgatataata actccttgta
     4621 ataaaactaa taaagaaatt gataagttta acaagagaga aattttagtt agagaaaaaa
     4681 agataagtag aatcatagta ataataaatg ctaagatacc tggtattcca aatcgtgaaa
     4741 taagataacc agttttacca agcggagtta ctattaaata tcttgaaatg ctgtcatcaa
     4801 tttctcccaa tatgaccatt gtagaagcaa aaaagaacat aaaaggtgta ataaaagcta
     4861 aaagtaaatc aaacatcaag aaatacggct ctaaatttaa ttgataataa aacttgttaa
     4921 gtgatatatt ctgtattaat ggtattccaa attttataaa tactccacat aatattggag
     4981 aaacacatac tataaaaagc atagcatctc tcttaatttg ttcaaactct tgtttaaacg
     5041 catttataat ctttatcata gttttactcc acctaccttt ttccaactgc tacaaataaa
     5101 ataataagtt ataaaataaa ttattattat aattgatatt aaaattaaaa tattaatcat
     5161 tataaaattt ttatctccac taataagaga aatacatata ttaaaagggt acaaatttgc
     5221 atatgatttt gtgtcaagaa gtacattcag tattggtgga ataaagcata taatctcaat
     5281 aggaatactt aaaacaataa actgattcaa actagatgct ttacttgcaa ttatcaatcc
     5341 aagcaaagaa aatataatag aagaaaaaaa ggtacctata gttgatatta taatattatc
     5401 taaatttaat gtaatagcaa taaacatagc tacaatacta gatattactc ctaatgaaat
     5461 tacctttgat aaaatatact cagatacttt tactggagat acagctaagg aatttaaaac
     5521 cctttgactt ttctcaagaa gtacaatagc tcccataaaa aacaatccca ttgctgctgg
     5581 gtcagaataa atcataatta tagctatttt ttctctcata aaagttggaa atacattgat
     5641 aatacaaata tatataatac ttaaaaaaag atataaaaaa taaaaaccat acttaaattg
     5701 aaactttata tctcctaaaa taagattttt tagcctcatt gtaaattcct ccctgttatc
     5761 tcaataaaaa tatcatttaa tgttggttca ctactatgta ttgataaaag tttatttttc
     5821 tcaataagca tattcaaatt tttatcatta gcagtattgt tcagaaaaca ttcagaagtt
     5881 ttttctccat tatcaaagta agtatatcta actttaatag ctccttttga cataataaga
     5941 ttatgtggtg tatctaaagc agatatttta ccatttacaa tgaacgctac tctgtcacaa
     6001 agctctgttg catctaacat gttatgagtt gttaaaataa tagtcttacc tttcgatttt
     6061 tctgataaaa ttatatcttt cataaccttg ctatttgatg ggtcaagacc acttgttggt
     6121 tcatctaaaa ataaaatatc tggattatgt aataaagctt ttataaaatt taatcgagat
     6181 ttcatacctt ttgaatactc agataccctt ttatttgatt cattttctag tcctactgat
     6241 tttaataact catcaataga tagtagtttt ttagaatata gagacccaaa atattttaaa
     6301 ttttctattg cagtcaactt ttcatataaa ctaggaaatt caaaatcaac tcctatattt
     6361 tcataaaatt tatttgagtg tcttttactc tctactccat ttacaatgac acttcctcca
     6421 taattagtta tcattccaat tagaatcttt tgtaatgtag attttcctgc acctgatggt
     6481 cctaaaaatc ctagtatttc cccttttcct acttcaaagt taatattttg taaaaaatct
     6541 ctatctgtat aactaaatga taaatcatca actttaatca ttcaatatca ccctttcaaa
     6601 tttttgacta ttttgactat atggtcaata aaatataatt tttttgtttg ttaataattt
     6661 ttaataaaag ttattagcaa acaaaattta aattctattc cataagttgt ataactaatc
     6721 cgtttatcat taatcttaaa gcatcttcaa aacaattact tccaacctcg cgcttatata
     6781 gcatggtaag aaaaactgct ctaaatgctc cactaaagct ttctatgtct ttattttttg
     6841 catttggtat gcaagatata atctctccaa tttcaaagtc atcatgtcta aagtattctt
     6901 caattataga atctggtaac tttctcacca atatctcaaa ttctccattt atcatgagat
     6961 ttaaaattga agtactatca accatcctat agaatttcat aaatatatct gtcaattgtt
     7021 cacatgtaat actttcatct actctattta tctcatataa gatttctttt tgtatagagt
     7081 catgtatatc tcttaatacc tcaaaaaaga gtaattcttt tgattgaaaa aatagataaa
     7141 aagttccttt tggtattttt acccgtttta caagctcatc aacagtagtt tttctaatac
     7201 catattgctc catacacaat cttgcttctt ctttaagtct ttttattata tactctcttt
     7261 ctttatcaga atatgattta ggcatctttt ccctccagtt tgactaaaat aatttatata
     7321 gtcaatataa tactttttct atctaatgtc aatagtatta ttagtatatt taaaaataag
     7381 cttaaaaact ttcgaaaatt ctgaatttta tatagcattt tttattatct ag
//
LOCUS       DAEPIN010000124         7383 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.126, whole genome shotgun sequence.
ACCESSION   DAEPIN010000124 DAEPIN010000000
VERSION     DAEPIN010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7383
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.126"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            493..1185
                     /locus_tag="KR250_003550"
     CDS             493..1185
                     /locus_tag="KR250_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0069433.1"
                     /translation="MYNILVVDDDKEIVDSIEIYLKSEGFKIYKAYDGMKALEIITEN
                     DIHLILMDIMMPKLDGIKATVKIREERNIPIILISAKSEDTDKIIGLNIGADDYITKP
                     FNLLELIARVKSNLRRYVTLGNYEDTCKEILKSGALELNTLTKEVKIDGENIKMTPIE
                     YKIIELLLENKGRVFSIDEIYEKVWNEESFNSENTVAVHIRRIREKIEINPKEPRFLK
                     VVWGVGYKIEKF"
     gene            1381..3441
                     /locus_tag="KR250_003551"
     CDS             1381..3441
                     /locus_tag="KR250_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBH0069434.1"
                     /translation="MEKYRVLKLVSLIIISISFPLTILLVTIGLAEPKYNIQKTSTKS
                     GIFDISDDFYDSNIFDEGTMASLLPKFKLSVGGKLTSKEKGKFQQEGKSIDLSSSDSE
                     YDKIMINEQASEEYLSYLKNIKVYVINTYTGVFYTNTSYASLYDFKQKTKSYCNVEII
                     NKIGKSSYIKTINGEKFELNNLSLELNEVDEGFEAYVSFPEEPTIEDGIVYTNFQIFK
                     QATEKVRLFFVASLSLAIICLLSIILYIKIKGEVLKRNNVFLLIYSKIPMEFNVILVS
                     ISIAILTIYALAVSYHIDVIALVSLSLLIVSNNIFFINVQLSYLENKMCIFKTSIIVR
                     VILWLIKTVREVSNAIKKVSLARKVIVIVIISLALIMAISTRSIGMGLLFSICIFLGF
                     TIYITKRLSYLSYVIEGTERIKKGELDYKIKITGNDNFTSLAENINNIGEGLDRAIEE
                     QVKSERMKSDLITNVSHDLKTPLTSIINYVDLIKKEESIQPEYINDYINVLESKSKRL
                     KLLIEDLFEASRVSSGNIELQISKIDLVQLLRQSIGELEEKLSKNNLYLKLNVPNDKV
                     YIWADGRRMYRVFENLLSNIAKYSLEETRVYIDVYDYGENVKVTMKNISSYELNFDPS
                     EIMERFKRADESRNTEGSGLGLAIARDLVALQGGKFYIEIDGDLFKANLEFQKYEEEI
                     TNME"
     gene            complement(3647..3970)
                     /locus_tag="KR250_003552"
     CDS             complement(3647..3970)
                     /locus_tag="KR250_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HBH0069435.1"
                     /translation="MNNNYIFFIIIGMYIVTYLPRVLPMLIFSKKEMPDFLKKIMKFI
                     PVAILTSLTAKDVFFNGDSLYLSIANPKIISFMIVLLVAYKFKSIGISIITGVISIYL
                     FGIIL"
     gene            complement(3963..4664)
                     /locus_tag="KR250_003553"
     CDS             complement(3963..4664)
                     /locus_tag="KR250_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HBH0069436.1"
                     /translation="MERTQQLKESFIASIPIFLGYIPIGIVGGILLQKSGLSPFQIAL
                     MVTLVFGGSSQFIAASMISTGASVTSIVLTTFIVNLRHFLMSSNLNMYIKNKSPKFIL
                     PFCHTITDETFAVNYEKFTAGNWSDRNAIYLNFFCLISSVLANFIGAFLGESISIDSS
                     ISGFILTSMFIALIVSQIKNKIYIVVFISSIIISVILYALFKSNLVIIVASILASLIG
                     FFIEESYCKKEESYE"
     gene            complement(4942..6327)
                     /locus_tag="KR250_003554"
     CDS             complement(4942..6327)
                     /locus_tag="KR250_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBH0069437.1"
                     /translation="MPINSFENYPMNWKPKRPSKGQILYKALVEQLEQDINNGFLLPG
                     TKLPPQRELADFLDVNVSTISRAFKICEKKGLISGVTGSGTFVSYDTRSNLFLMSSNN
                     KITFIEMGTMNPDFTLEEMNTLFKHIIKEIDFKTIFQYGQRDGAKWQKEAIAKLIYKA
                     GLETTSDSLLPASGGQNAIVAILAGLFQHGDRIGVDPLTYPGIKTAAKMLGIQLIPIK
                     QEDNEISEEGLLYACKNENIKGLYIIPDYQNPTTHIMSQNGRKMIANIASKYNLIVIE
                     DAIHSLLNETHLNPVASYLPNQTIYITSLSKIIAPSLRLAYISTPKQYREALSSALYN
                     INLSQSYFLTEIAYRMITSGEADKLINARRKSARRRNKIINQYLSGYNLLGNEECIFR
                     WLILPDGIMAEKFEIQALKEGVQVYASERFAVGKEKPISAIRIAVCATESTEELKAGL
                     SVLKRLLEEKK"
     gene            complement(6435..6578)
                     /locus_tag="KR250_003555"
     CDS             complement(6435..6578)
                     /locus_tag="KR250_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069438.1"
                     /translation="MYDCDNQPKTPEEAVLYTRKRNFNIFKKTIPLYDKEGIKIIGRF
                     EIN"
     gene            complement(6773..7195)
                     /locus_tag="KR250_003556"
     CDS             complement(6773..7195)
                     /locus_tag="KR250_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HBH0069439.1"
                     /translation="MNTFKTEVLLKYSAKDIFNIFTKSARLNFPNFNEKKAVGTFVQQ
                     KNNKRFKVEITNFEKNRVYEIKTSNKRESTITRYELVHIDLENTKLIFIESESKRNIF
                     GKINSALVRVLFGKRQPEEFNKFIKKLEMELENNSVNL"
BASE COUNT         2855 a          841 c         1030 g         2657 t
ORIGIN      
        1 ttatataaat tttaatagaa tatgtacagt atatagtaga tagaatgtat aaaaatagaa
       61 tttaaataag tagctattat tgcagtgaaa tgaatgtttc taagtatatt tgggtggaag
      121 aatggtggaa gaaaattagt gaaatgtaat tcttccacca ttcttccacc caaaaaataa
      181 aaactgttca ataagaatta atatacatca attcagctga atgcaatcaa aatacttaaa
      241 tgaaagtaat ctgaaaacaa cataattcaa atcaatttat tcaaataaac ctcaatttat
      301 cttaaatctt aagaaatatt taagaattag gataatattt tcttaagatt tatattttat
      361 tatatagtta taagggaaag ttacaaaaat gtaagccaaa gtaatataac aatttagtca
      421 ttgttgagaa agggagtgaa agatattgta taatatagat aagtattatt atacaataga
      481 ggaagtgaaa atatgtacaa tatattagta gtagacgatg ataaagaaat tgttgattcg
      541 atagaaatat atcttaaatc agaaggattt aaaatatata aagcatatga tggaatgaaa
      601 gctttggaaa taataacaga gaatgacatt catctaatac taatggatat aatgatgcct
      661 aaattagatg gaatcaaagc aactgtaaaa atacgagaag aaagaaatat tccaataatt
      721 ttaatttcag caaaaagtga agatacagat aaaataatag gattaaatat tggagctgat
      781 gattatataa caaagccatt taatctatta gagcttatag ctagggttaa atcaaactta
      841 agacgatatg taactttagg taattatgaa gatacatgta aagaaattct aaaaagtgga
      901 gctttggagt taaatacact aacaaaagaa gttaaaattg atggtgaaaa cataaaaatg
      961 actccaattg agtataaaat aattgaactt cttttagaaa ataaaggacg agttttctca
     1021 atagatgaga tatacgaaaa agtatggaat gaggaaagtt ttaattcaga aaatacagta
     1081 gcagtacata tcagacgaat aagagaaaag attgaaataa atccaaaaga accaagattt
     1141 ttaaaggtgg tgtggggagt tggttataag attgaaaaat tttagtggaa aaattataaa
     1201 agaaaagagt ttaatattta attgcatttt gattttagtc atttttacat ttacacaaat
     1261 gttaggaatt ttaatatgtt ttaatttttt ctctgagagt ttaatttact ctatggggat
     1321 tatgcttttc ttaaacttta ttttatataa aatactgaca atgtagatga ggtgggcaac
     1381 ttggagaaat atagagtatt aaagttagtt tcattaatca taatatctat ctcatttcca
     1441 ttaactatat tgttggtgac aataggactt gctgagccta aatacaatat acaaaaaact
     1501 tctacaaaga gtgggatttt tgatatatct gatgattttt atgatagtaa tatatttgac
     1561 gaaggaacaa tggcaagtct attacccaag tttaagctat ctgtaggtgg taaattaact
     1621 agtaaagaaa aaggtaaatt ccaacaagaa ggtaaaagta tagatttaag tagtagtgat
     1681 tctgagtatg ataaaatcat gattaatgaa caagcttctg aagaatattt aagttatcta
     1741 aaaaatataa aagtgtatgt tattaataca tacacaggag tattttatac aaatacgtca
     1801 tatgctagtt tatatgattt taaacaaaaa actaagagct attgtaatgt agagataata
     1861 aataagatag gaaagagctc atatattaaa acaataaatg gagaaaaatt tgaactgaat
     1921 aatctgagtc tggaactaaa tgaagtagat gaaggttttg aggcatatgt atcttttcca
     1981 gaagagccaa ctatagaaga tggaatagta tatactaatt ttcaaatatt taaacaagct
     2041 acagaaaaag taagattatt ttttgtagct agtttaagtt tagcaattat atgtttatta
     2101 tctataattt tatatatcaa aataaaaggt gaagttttaa aaagaaataa tgtattttta
     2161 ctaatataca gtaagatacc tatggaattt aatgtaatat tagtatctat ttcaattgca
     2221 attttaacta tatatgctct tgctgtatct tatcatattg atgtaattgc attagtttct
     2281 ttatctttac tgatagtatc aaataatata ttttttataa atgtgcaatt gagctattta
     2341 gaaaataaaa tgtgtatatt taagacaagt attatagtaa gagtgatttt atggttaata
     2401 aaaactgtaa gggaggtcag taacgctata aagaaagtat ctttagcaag gaaagttatt
     2461 gttatagtga taatatcttt agctttaatc atggcaatta gcactcgaag tataggtatg
     2521 ggattactgt tttctatatg tattttttta ggatttacaa tatatataac taaaagactt
     2581 tcatatctta gttacgtaat agaaggtaca gaaagaataa aaaagggaga attagattat
     2641 aagataaaaa ttactgggaa tgataatttt acgtctttag ctgaaaatat aaataatatt
     2701 ggagaaggtc ttgatagagc tatagaagag caagtaaaaa gtgaaagaat gaagtctgac
     2761 cttataacaa atgtatctca tgatttgaaa actccactta cttctataat aaattatgta
     2821 gatttaatta aaaaagaaga atcaatacaa ccagagtata taaatgatta tataaatgta
     2881 cttgagtcaa agtcaaaaag actaaaatta ttgatagaag atttatttga agcgagtaga
     2941 gtaagtagtg gcaatataga attacaaatt agcaagattg atttagtgca actattaaga
     3001 caaagtatag gtgaacttga agaaaaatta tctaaaaata atttatattt aaaattaaat
     3061 gttccaaatg ataaggtgta tatatgggca gatggaagaa gaatgtatag agtatttgaa
     3121 aatcttttat ctaatatagc aaaatattcc ttagaagaaa ctagagtcta tattgatgta
     3181 tatgactatg gtgaaaatgt aaaggtaact atgaaaaata tatcttctta tgaattaaat
     3241 tttgacccaa gtgagattat ggaaagattt aaaagagctg atgaatcaag gaatactgaa
     3301 ggaagtggat tagggcttgc tattgcaaga gatttagtag ctttacaagg tggaaaattt
     3361 tatatagaaa tagatggaga tttatttaaa gcaaatttag agtttcaaaa atatgaagaa
     3421 gaaataacaa atatggaatg agatagtatt taagtatata tatttttttt actgtcgaaa
     3481 gcttttatta taatatatta gaatatttac agtagagaat gaagagtaaa aacagagtaa
     3541 ctttaagtca ctctgttttt tatttaaaat caataatata acaacttaaa ttaaaattgt
     3601 acacatagtt ttaaaataac ttatacaata aaacattatc caaatatcac aagataattc
     3661 caaataaata tatagatatc actcctgtaa taatagaaat accaatagat ttaaacttat
     3721 atgcaacaag aagtacaatc ataaaagata taattttagg gttagctata gataaatata
     3781 gactatctcc attaaaaaat acatctttag cagttaagga agtaagtatt gctacaggta
     3841 taaatttcat gatttttttc aaaaaatcag gcatctcttt ttttgaaaaa attaacatag
     3901 gaagcactct tggtagatat gtaacaatgt acattccaat tataataaaa aatatataat
     3961 tattattcat aagattcctc ctttttacag taagattctt ctataaagaa accaattaaa
     4021 gatgctaaaa ttgatgcaac tattattact aaattgcttt tgaataatgc atataaaata
     4081 acagaaataa tgattgatga tataaatact acaatataaa tcttattttt tatttgagaa
     4141 actattaaag ctataaacat agaagtcaat atgaagccag aaatagaact gtctatagat
     4201 atactttctc ctaaaaatgc tccaataaaa ttagctaaaa ctgatgaaat taagcagaag
     4261 aaattaagat aaatagcatt tctatcagac caatttccag cagtaaattt ttcataatta
     4321 actgcaaaag tttcatctgt tatagtatga caaaatggca atataaattt tggactttta
     4381 ttttttatgt acatgttcaa attagaactc attagaaaat gtcttaagtt aactataaat
     4441 gtagtcaaaa caatagatgt tacacttgca ccagtagaaa tcattgaagc tgcaatgaat
     4501 tgagaacttc ctccaaatac taaagttacc attagtgcta tttgaaaagg tgaaagacca
     4561 gatttttgta atagtattcc tcctactata cctataggta tataacctaa gaatatagga
     4621 attgaagcaa taaagctttc ttttaattgt tgagttcttt ccacaataaa tcactccttt
     4681 tgtattaaat gtattaaagt attagtaaaa cagaaaaata gattaattgt ataattgcaa
     4741 ttataattaa aaataaattt ggattaatat ataaaatact atacaacaaa tagataatct
     4801 taaacggtta ttaaatttta tctatttgtt attatgatgt aataatacat attaatctct
     4861 ataagttaat tactatctgg tgtaatttta ttttaacaac aaaaaaaaat acacacaatt
     4921 aaaatattgt gtgcaataca attatttttt ctcttctaat agtcttttta atacgctcaa
     4981 accagcttta agttcttctg tactttctgt agcacataca gctattctta ttgcagaaat
     5041 tggtttttct tttccaacag caaaacgttc agaagcatag acttgtacac cttctttaag
     5101 tgcttgtatc tcaaattttt ctgccattat tccatcaggt agaatcaacc atctaaatat
     5161 acattcttca tttcccaaaa gattatatcc agataaatat tgattaataa tcttgtttct
     5221 tcttcttgca gatttacggc gtgcattaat taatttatcg gcttcacctg atgtaatcat
     5281 gcgataagca atttctgtta aaaaataaga ttgagataga ttaatgttgt ataatgcgct
     5341 tgataatgcc tctcgatact gtttaggtgt agagatatat gctagccgta aactaggtgc
     5401 aataattttt gataaactcg taatataaat tgtttgattt ggcagatagg aagccactgg
     5461 atttaaatgt gtttcattaa gtaaactatg aatagcatcc tcaataacaa ttaaattgta
     5521 tttagatgca atatttgcaa tcatttttcg tccattttgt gacattatat gtgtagttgg
     5581 attttgataa tctggtatta tgtatagacc tttaatattt tcatttttac aagcatataa
     5641 tagtccttct tcactaatct cattatcttc ttgtttaatt ggaattagtt gaatcccaag
     5701 cattttagct gctgtcttta ttccaggata agttaatggg tctacaccta ttctatctcc
     5761 atgttgaaac aaaccagcta aaatagcaac aattgcattt tgtccaccac ttgcaggaag
     5821 taagctatct gatgtagttt caagcccagc tttataaatt aatttggcaa tagcttcttt
     5881 ttgccattta gcaccatctc tttgtccata ttggaatata gttttaaaat ctatttcctt
     5941 aattatgtgt ttaaacaaag tattcatttc ttctagcgtg aaatctggat tcattgtgcc
     6001 catttcaata aatgtaattt tattattact ggacatcaag aatagatttg aacgtgtatc
     6061 ataagataca aaagttccac ttcctgtaac tccacttatt agaccttttt tttcacaaat
     6121 tttaaaagct ctagagatag tactcacatt aacatctaag aaatctgcca attcacgttg
     6181 gggtggtaat ttagtacctg gtaataaaaa tccattgttg atatcttgtt ctagttgttc
     6241 tacaagcgcc ttatataata tttgcccttt tgaaggtctc tttggtttcc agttcatagg
     6301 ataattttca aaagaattaa ttggcataag ttttcacctc acattattaa tttgattttt
     6361 tctttatcta aaacaataaa ttctaaagat acaaataaaa actgatagga aataattcta
     6421 tcagtttttg tcttttaatt gatttcaaat cttcctataa tttttatacc ttctttatca
     6481 taaagaggga ttgttttttt aaatatatta aaatttcttt ttcttgtata taaaacagct
     6541 tcttctggtg tctttggttg gttatcacaa tcgtacaaat ttcttttttt taactaaaag
     6601 caataataca taaaattatt attgctaatg atactcttaa tgtggtatat agtcatagtg
     6661 tcacatgtct atcaatagtt ttttcagtaa ttattgtaac gaatagtagg actcataaaa
     6721 tacttagaaa tatgttttta gataaatttt aaatatataa tctaacttat atctataaat
     6781 taacactatt attctccaat tccatttcta gtttttttat aaatttatta aattcttctg
     6841 gttgcctttt tccaaacaat actcttacaa gagctgaatt gatttttcca aatatattcc
     6901 tcttgctctc actttcaata aaaataagtt ttgtattttc taagtcaata tgtactaatt
     6961 catatctagt aatagtactt tctcttttat tgcttgtttt tatctcatat actctatttt
     7021 tttcgaaatt agttatttca accttaaatc ttttattatt tttttgctgt acaaatgttc
     7081 ctactgcttt tttctcatta aaattaggaa aatttagtct tgcagatttt gtaaatatat
     7141 taaatatgtc ttttgcagaa tattttaata aaacttcagt tttaaatgta ttcatatcgt
     7201 tttcccttct gtatataaat ttaatattta taagttaatt tttaagttaa aatagtataa
     7261 tatgccatat taaatcatgt taaaactatt tatatagcta aaatcttacc tatataagta
     7321 tgtatactat catataaaaa taaaatctta aaatagaaag ggttaaaaac acagtttatt
     7381 ata
//
LOCUS       DAEPIN010000125         7287 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.127, whole genome shotgun sequence.
ACCESSION   DAEPIN010000125 DAEPIN010000000
VERSION     DAEPIN010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7287
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.127"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            complement(<1..762)
                     /locus_tag="KR250_003557"
     CDS             complement(<1..762)
                     /locus_tag="KR250_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HBH0069440.1"
                     /translation="MTFKNKKFTINKYKILSIILVLCLILTAFSSYVNYSHKKFKDYV
                     VFYVQHQDDEVLWAGSAIVNAIKERGKNHVFVVLVSTGCGISVFDKYKKYENLTNIQK
                     SEYRNREFLASLNSLGVIRENIILLPETNTNGNTDFNYMEKIALTFEEKFKNVTHIAH
                     TYKLDDHLQHLKNGSVIQNLYNAGKIKDAKYFVKPKFADKITFNNKIVYKAHSTEDYE
                     KVKNACQQYKIVDELEYREGIGYKSDHKSFDKLLNN"
     gene            complement(1076..1807)
                     /locus_tag="KR250_003558"
     CDS             complement(1076..1807)
                     /locus_tag="KR250_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069441.1"
                     /translation="MNIFDILRDYLLVQVDKYNLNMDMISIVSKSLSSKEAIGNTKRK
                     DFPIIVGKEIMLEADFKGAKGQAFTSTPSTFEGSLKDILSLDLHDNSHDRSLFIASLN
                     AVMKYLGKTDRTIHCKNNEPEVCAKKFPEFIKMEFGNPKVAIIGYQPAIIDNIKDFFE
                     TRVLDLNPEFVDTIQYNVKIEDGIRDYEDVISWADLVICTGSTLCNNSIINFLSLNKP
                     VYYYGTTIAGASNILGLKRLCFCSK"
     gene            complement(1794..2489)
                     /locus_tag="KR250_003559"
     CDS             complement(1794..2489)
                     /locus_tag="KR250_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBH0069442.1"
                     /translation="MLKIKNLSLDLPNFKLQENSLNINNHEYFVLLGASGSGKTLFLE
                     TLAGRYPSAKGNVSYKENLISNLPPENRNIGFVYQNFELFPNMNVEDNIRFPLKLRKI
                     PIDEQILKANHLSKLMQIEDIKNRYPKNLSGGEKQRVAFARALIAEPDILLLDEPMSS
                     LDYITKKHISKILKDVYIKYKPTVIHVTHDISEAMFFAHKIGIMNNGKINHIFDLNEE
                     IKNKGESFFYEYI"
     gene            complement(2491..3282)
                     /locus_tag="KR250_003560"
     CDS             complement(2491..3282)
                     /locus_tag="KR250_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0069443.1"
                     /translation="MKNKFFTTLLTGFFAIVFLFIVSPLLMLIIKGISYVPICLKSVE
                     VQYAITLSIKTSLISTIVCLLLAIPVAYFLHITKLPFKKLIIQIINLPMSLPHLVSGI
                     ALLLLFGRMGIGDSIYKIFKLDFIFTKQGIVLAQIFVNLPLTIKILHTSLNESNEKMI
                     FVARTLGCNSWEAFRFIILPNLKTGIISATVMTWSRALGEFGAVAMIAGSTRMKTEII
                     PTSIYLNMSTGDIDIAIGIAVILIFLSLTCLMLFEIFFNREVDKN"
     gene            complement(3292..4104)
                     /gene="modA"
                     /locus_tag="KR250_003561"
     CDS             complement(3292..4104)
                     /gene="modA"
                     /locus_tag="KR250_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBH0069444.1"
                     /translation="MFKKLSVLALAMIISLSITACNSKENKANDESNTKVTTADDSKE
                     KETLFVYSGAGLKKPMDEIAKKFEKENNVKVEYSYAGSAQLIAQIETSHKGDVFIVGS
                     EPIYSKAFEKELVGEHKTVAHHTPAIVVPKGNPANIQKLEDLNKDGVKLILGDKESNA
                     IGKTTQKILEKNNLKSINDNVVSTAATVNEMIVQLTSSKADATIATKDSVFGNEDVEV
                     IEIAQDKNIDQLISAGIVNYSDKKDLANKFINYIESDEAKQIFKKYGFEPVK"
     gene            complement(4739..4939)
                     /locus_tag="KR250_003562"
     CDS             complement(4739..4939)
                     /locus_tag="KR250_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003723180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBH0069445.1"
                     /translation="MNNGIVKWFNNEKGFGFISMESGDDVFAHFSAIQTSGFKSLEEG
                     QKVSFDIVKGARGPQAENITIL"
     gene            complement(5164..5340)
                     /locus_tag="KR250_003563"
     CDS             complement(5164..5340)
                     /locus_tag="KR250_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069446.1"
                     /translation="MFRDKMDKCTHMLTAYIGSSYDYCDFIDTQLDDFILEYGEKVVE
                     SCLHQVMVLVSKYN"
     regulatory      complement(5394..5483)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(5663..5863)
                     /locus_tag="KR250_003564"
     CDS             complement(5663..5863)
                     /locus_tag="KR250_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0069447.1"
                     /translation="MPKFTCNLKKYRQLKELTQEQLAEKVGVRRETIMRLEAGKYNPS
                     LKLAIDISKIVDTPIDELFIFD"
     gene            complement(5865..6215)
                     /locus_tag="KR250_003565"
     CDS             complement(5865..6215)
                     /locus_tag="KR250_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3796 domain-containing protein"
                     /protein_id="HBH0069448.1"
                     /translation="MIQKKFFMGFCGFLGFLSLRYFSSGNVADLTYIGFFAFFSNFII
                     SKINGDKADERYVQDEKAAMAFTGQLAIIELFILWCITIVSRNIELMCVLLSITYAIT
                     LNVYAIKLYILEEK"
     gene            6377..6566
                     /locus_tag="KR250_003566"
                     /pseudo
     CDS             6377..6566
                     /locus_tag="KR250_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890498.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(7043..7213)
                     /locus_tag="KR250_003567"
     CDS             complement(7043..7213)
                     /locus_tag="KR250_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069449.1"
                     /translation="MHVYQPKTSEYLLNTYAKSYLSSIVFLFDYITKNESGYAFFIID
                     FPFLSLDIFYKF"
BASE COUNT         2651 a          993 c          862 g         2781 t
ORIGIN      
        1 attatttaat aacttatcaa atgatttatg gtcagactta tagccaatac cttctctata
       61 ttctaattca tcaacaattt tgtattgttg acaggcattt ttaacctttt catagtcctc
      121 agtagaatga gctttataaa ctatcttatt attaaaagtt attttatcag caaatttagg
      181 ttttacaaaa tatttagcat cttttatttt accagcatta tataaatttt ggataacaga
      241 accatttttt aaatgttgta aatggtcatc taatttataa gtatgtgcta tatgagttac
      301 atttttaaat ttttcttcaa atgtaagagc aattttttcc atgtaattga agtcagtatt
      361 accatttgta tttgtttctg gtaataatat tatattttcc cttattacac caaggctatt
      421 aagagaagct aaaaattctc tatttctata ttctgatttt tgtatatttg ttaggttttc
      481 atatttttta tatttatcaa agacggatat accacatcca gtagatacta aaaccacaaa
      541 tacatgattt tttcctcttt cttttatagc attaacaata gcactcccag cccacaacac
      601 ttcatcatct tgatgctgaa catagaagac aacataatct ttaaattttt tatgactata
      661 attcacatag ctactaaaag cggttaaaat caaacataaa actagaataa tagatagaat
      721 tttatattta tttatagtaa attttttatt cttaaatgtc atttaatcat ctcctgaaag
      781 gttgtcttag ttatattatc aaacaaagta ctaaaaatta tttcttaaat gctgaaaaat
      841 atagagtaga ttaatggaat aaaatttttt tattagttta aaataaagaa aacatttatg
      901 tataagacta atcaaaatat taatgattga taggaattga gatttaaagg gattgtacat
      961 ttattcggga ttcattttaa tagaatttta ctttagtact tagaataaaa gtaataactt
     1021 tttaaattta aagagtacac atatatattt gtgtactcct tgatgatatg attgcttatt
     1081 tactacaaaa acagagtctt tttaaaccta aaatatttga tgcaccagct atcgtagttc
     1141 cataatagta aactggtttg ttaagtgata gaaaatttat aattgagtta ttacataatg
     1201 tacttcctgt gcaaatcact aaatcagccc aagatattac atcttcataa tctcgtatac
     1261 catcttcaat cttaacatta tattgaatag tatccacaaa ttcaggattt aaatctaata
     1321 ctcttgtttc gaaaaagtcc ttaatattat caattatggc tggttgatat cctataattg
     1381 ctacttttgg attaccaaat tccattttta taaactcagg gaattttttt gcacatacct
     1441 ctggctcgtt atttttacaa tgaatggttc tatccgtctt gcctaagtat ttcataactg
     1501 catttaatga tgctataaaa agacttctat catgagaatt gtcatgtaaa tctaatgata
     1561 atatgtcttt taaagaacct tcaaatgtag atggagtaga agtaaatgct tgtcctttag
     1621 ctcctttaaa gtcagcttca agcattattt ctttaccaac tataattggg aaatctttac
     1681 gcttagtatt tccaattgct tcttttgaag atagagattt ggacacaata cttatcatat
     1741 ccatatttaa attatattta tcgacttgta ctaatagata atctctcaaa atatcaaata
     1801 tattcataga aaaaagattc tcctttattt tttatttctt catttaaatc aaaaatatga
     1861 tttatttttc cattgttcat tattcctatc ttatgagcaa aaaacattgc ttcagaaata
     1921 tcatgagtaa catgaatcac tgttggtttg tattttatgt agacatcctt caaaattttg
     1981 cttatatgtt tttttgtaat ataatccaat gaactcatag gttcatctaa aagtaatata
     2041 tcaggttcag ctattaaggc acgtgcaaaa gcaactcgtt gcttttcgcc tccacttaaa
     2101 tttttaggat acctattttt tatatcttct atctgcatta atttagacaa gtggttagcc
     2161 ttaagaatct gttcatctat aggtattttt ctcaatttaa gtggaaacct tatattatct
     2221 tcaacattca tatttggaaa taattcaaaa ttttgatata caaatcctat atttctattt
     2281 tctggaggta agtttgatat taaattttct ttgtatgata catttccttt agcagatgga
     2341 tatcttcctg ctaatgtttc tagaaataga gttttacctg acccactagc tcctaataaa
     2401 acaaagtatt catgattatt aatatttaaa ctattttctt gtaatttaaa attaggtaaa
     2461 tcaagagata aattttttat ttttaacata ttaattctta tcaacctctc tattaaaaaa
     2521 tatctcaaaa agcatcaagc atgtaagtga gagaaatatc aaaattacag ctattccgat
     2581 agcaatgtct atatcaccag tagacatatt taaatatata gatgtaggaa taatttcagt
     2641 tttcatacgt gtagaacctg ctatcatagc tactgcacca aattcaccaa gtgctcttga
     2701 ccaagtcatt acagttgcag atatgattcc agtttttaga tttggaagaa ttataaatct
     2761 aaatgcttcc caactattac atccaagggt acgtgcaaca aatatcatct tttcattact
     2821 ttcatttaat gaagtgtgaa gtattttaat agtaagtgga agatttacaa atatttgtgc
     2881 taaaactata ccttgctttg taaatataaa atcaagctta aaaatcttat aaatggaatc
     2941 tccaatcccc attcttccaa acaaaagtaa aagagcaatt cctgatacta agtgtggaag
     3001 agacataggg agatttatta tctgtataat cagtttttta aatggtaatt tagtaatatg
     3061 taaaaaatat gctactggaa tagctaataa aaggcatact atagtagata ttaaagatgt
     3121 tttaatacta agtgttattg catactgtac ttccactgat tttaagcata taggaacata
     3181 acttatccct ttaatgatta acataagaag tggtgataca ataaacaaaa agactatagc
     3241 aaaaaatcca gtaagtagag tagtaaaaaa tttatttttc aagtttaatc actacttaac
     3301 aggttcaaaa ccatattttt taaatatctg tttagcttcg tctgactcta tataatttat
     3361 aaatttatta gctaaatctt ttttatcact atagtttacg attcctgctg atatgagttg
     3421 gtctatattt ttatcttggg caatttctat aacttctaca tcttcattac caaagaccga
     3481 atcttttgtg gctattgtag catcagcttt acttgatgtt aactgtacaa tcatttcatt
     3541 tacagttgca gctgtagaga caacattatc atttatagat tttaaattat ttttttcaag
     3601 tattttttgt gtagtcttac ctatagcatt agactctttg tctcccaaaa taagtttaac
     3661 tccatcttta tttaagtctt ccaatttttg aatattagct ggatttcctt taggtacaac
     3721 tattgcaggt gtatgatgtg caaccgtttt atgttcacct acaagttctt tttcaaaggc
     3781 ctttgaataa attggttcag aaccaacaat aaatacgtct cctttgtgac ttgtctcaat
     3841 ttgagctatt agctgtgcag aacctgcata gctatattca actttgacat tattttcttt
     3901 ctcaaatttt tttgctattt catccattgg tttctttaaa cctgcccctg aatagacaaa
     3961 aagagtctct ttttcttttg aatcatcagc tgttgtcact ttagtattag attcatcatt
     4021 agctttgttt tctttagaat tacatgcagt aatacttaat gaaataatca tagctaaagc
     4081 tagtactgaa agttttttaa acataagata ttccccctta ttaaattagt gtaaattctt
     4141 ttttagtata tcatactaag aaaacgatgt ctaagttttt gttatgtttt ataatgtttt
     4201 gttatgtttt gttatgtata gttaagaaaa aatctattca taatgtcatt tccataggta
     4261 gttattttat ttttaatttt tattaatact ttaaagtatt ttaaatttaa aaacttaaat
     4321 caaattaact tattgtagat tttttacttt ttaactaatt ttatatttat tatatttcag
     4381 tgaaaaaata gaacattgaa acatatagat atgttttaat tgctttttat taacaaatta
     4441 ctaaaagtat tatatcagaa ttgaatacaa aagaaagagt atgtatttat gagatagttg
     4501 aaaattttga tgtgccacaa ctaaaattat tatattacta tatattctaa ttaaaagagt
     4561 acttgtaggt ttaagtacat actttataaa tatatttttg tcagatttat tttataaaaa
     4621 gtatttagac taagtattat ttttaagtat ttttttgttg aagttatttt ttcctttttt
     4681 acaaaatgcg taaataaaaa aaagactctt aaaagagtct ttccgaaaaa tctatatatt
     4741 ataatatagt tatattttct gcttgaggac ctctagcgcc tttaactata tcaaagctta
     4801 ctttttgacc ttcttctaat gatttaaatc ctgaagtttg tatagctgag aaatgagcaa
     4861 atacgtcatc tccactttcc attgatataa atccaaatcc tttttcatta ttaaaccatt
     4921 ttactattcc attattcatt aaaaatactc ctattaacct atatcaagat atacttgatt
     4981 tacttgtgta ttaccacaaa tactattgta tcatagatta acataaatgt ctatttaatt
     5041 ataattttta gttaacattt ttatttattc ttaatatgaa aataaattta aacattttat
     5101 attaccaata tattaatgta aatgtagata aaactaaaaa gggttttcta gaaaaaaatc
     5161 tgtctaatta tacttactta ccaataccat cacttgatgc aagcaagatt ccacaacttt
     5221 ttctccgtac tctaatataa aatcatctaa ctgtgtatct ataaaatcac aataatcata
     5281 tgaactacca atataagcag ttaacatatg tgtacattta tccattttat ctctaaacat
     5341 aatgtattta tccccctaaa aaactttcat gaaaaacaaa aagaaaatat ctttttggca
     5401 actggctgac ataacttatt ttataagcgt tagtccctat agctttgcgt cactaaattt
     5461 ctctagtttt gccgatttag ttttattcta cagattaata atacaataga attagttatt
     5521 attcaacata tctagctgaa aaattaataa atttatatat gaacgttaaa ttatctgaaa
     5581 aaataaaaag cccctctgtc aggagtgact taaaaattga atttaagttt gtatttaaaa
     5641 cacattttta ataatgcaaa aactaatcaa aaataaacaa ttcatcaata ggtgtatcta
     5701 ctatttttga tatatctatt gctaatttca aagaaggatt atactttcct gcttctaaac
     5761 gcataatggt ttctcttcta actcctactt tttctgctaa ttgttcttga gttaattctt
     5821 ttaattgtct atattttttt aggttacaag tgaatttagg catattattt ttcctctaaa
     5881 atataaagtt ttatagcata aacatttaaa gtgattgcat atgtaattga tagtaaaaca
     5941 cacattaatt ctatattcct agacactata gtaatacacc ataaaatgaa taattctata
     6001 atagcaagct gtcctgtaaa tgccatagct gctttttcat cttgtacata cctttcatct
     6061 gctttatcac catttatttt tgatataata aagtttgaga aaaaagcaaa aaatccaata
     6121 tatgtaaggt cagcaacatt gccagatgaa aaatatctaa gacttaaaaa tccaaggaaa
     6181 ccacaaaaac ccataaaaaa ttttttctga atcatttttt atcctcctta gtaattttaa
     6241 aagagatata tttatatctt tatgatacaa atatatctct tttatattct tttgtcaata
     6301 gaaaatcagt attatacaat atttgtactg caccataatt gttagagcaa aaatctactg
     6361 cttgagatgt ttatgtatga ctaaatatga ttttaatttc aaattaaaag tagctaaatt
     6421 ttatttgaat agagaaggaa gagatatttt aatagtaaaa caatatggag tttataacca
     6481 ttctcaagtt gaaagatggg ttaatatatt aggagagtgt gcactaaaaa taaaaaaaat
     6541 tttctatact atatggttca agttagatgt tataaactat atgatgataa ctaaatatag
     6601 tgtacaataa atcgctaagt attttggaat aaacaatagc acagttatag caacatgaaa
     6661 ataaaaattt ttacatagtg gtgttgatgc cttttctaaa atgaaaggaa gaccataatg
     6721 aataaaaaca aaaacactaa agaaaaaata aattaacaag agaacaagaa ttagaatatg
     6781 aaaataaatt tttaaaagat gagcttgagt ttttaaaaaa gatgtgtgcc tcagggaagc
     6841 cacattaaaa aacagtggta tcattattaa tcaaaagaaa gttagatgta ttatcaaaaa
     6901 attagaactt aaatgcatag cattttttca taaatcacat aaatataact cctataaatg
     6961 tataattgga aaagtaaaaa ataataaaac aaatagaaga tttaaaatat caatagcata
     7021 tcagaaaata acaaatgata tgctagaatt tataaaatat atctaatgat aaaaatggaa
     7081 aatcaattat aaaaaaggca tatcccgatt cattttttgt tatatagtca aataagaata
     7141 ctatagatga aagatacgat tttgcatatg tattaagtaa gtattctgat gtttttggtt
     7201 gatacacatg caataaagaa gttgtgtttg cagataaatt agcaaaacta tagtataagt
     7261 tgcaacaata aagaaaatga aaattat
//
LOCUS       DAEPIN010000126         7073 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.128, whole genome shotgun sequence.
ACCESSION   DAEPIN010000126 DAEPIN010000000
VERSION     DAEPIN010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7073
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.128"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            88..161
                     /locus_tag="KR250_003568"
     tRNA            88..161
                     /locus_tag="KR250_003568"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:120..122,aa:Gly,seq:tcc)
     gene            170..286
                     /gene="rrf"
                     /locus_tag="KR250_003569"
     rRNA            170..286
                     /gene="rrf"
                     /locus_tag="KR250_003569"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            560..2578
                     /locus_tag="KR250_003570"
     CDS             560..2578
                     /locus_tag="KR250_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBH0069450.1"
                     /translation="MRRNTKLLTTGILSMAIVAPTMAFATESNAMENNADLNINLEKK
                     SIVLGSTSKVSVKFKEKPDADSITLKYKCYDMPLDTTLNYNQSTESYEGTINYNKDPE
                     YLNVWELQGITINSKNNPKTLNKQELEKMGLNLKDYNVTQECIIEDITSRKDVNKYLR
                     KTSAPITELTGSDRYETAVKISKEGWKNGSDKVVIINGDVSIDGIISTPLATTYNAPI
                     LLVEKNNVPNSVKSELKRLNPRDVIIIGDENAISKTTANQIKSTVNASQTRLKGSNRY
                     ETSLLIAKEIDKNHDVEKVYITNANGGEVDALTIAAKAGQDKQPIILTDKNSITDNTY
                     KWLKSEDLQNAYFIGGPQMISTNVINKVNDITKDNVTNNRVYGADRHETNANVIKKFY
                     TDDELEAVLVAKSDVLVDALAAGPLAANLKSPILITPKTYVSAYHKENLEAKSANKVY
                     KIGGGLTSKVMNSIASSLSKHNTTPTEPGNSGGKTVMIDPGHGGSDTGTTGKPLGGIK
                     EKDYTLNTSLATTEYLRSKGFNVIMTRDTDKTLSLGNRTALSNSLRPDLFTSIHYNAS
                     DTTGNGVEVFYKLKDKDGGTTKTVATNILNRILEKFNLKNRGAKTRTLSTDPTKDYLY
                     VLRNNDMPAVLVECAFLDNEKDMSLLNTSNKVKEMGTQIGKGIEDSLK"
     gene            3086..5098
                     /locus_tag="KR250_003571"
     CDS             3086..5098
                     /locus_tag="KR250_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBH0069451.1"
                     /translation="MRRNTKLLTTGILSMAIVAPTMAFATESNAMENNADLNINLEKK
                     SIVLGSKSKVSVKFKEKPDADSITLKYKCYDMPLDTTLNYNQSTGAYEGTINYNQDPE
                     YLNVWELQGITINSKNNPKTLNRQDLEKLGLNLKDYNVTQECIIEDITSRKDVNKYLR
                     KTSSPITELTGSDRYETAVKISKEGWKNGSDKVVIINGDVSIDGIISTPLATTYNAPI
                     LLVEKNNVPNSVKSELKRLNPKDIIIIGDENAISKTTANQIKSTVNASQTRLNGSNRY
                     ETSLLIAKEIDKNHDVEKIYITNANGGEVDALTIAAKAGQDKQPIILTDKDSITDNTY
                     KWLKSEDLQNAYFIGGPQMISTNVINKVNGITKDSVTNNRVYGADRHETNANVIKKFY
                     TDDELEAVLVAKSDVLVDALAAGPLAANLKSPILITPKTYVSAYHKDNLEAKSANKVY
                     KIGGGLTSKVMSSIASSLSKHNTTPTEPGNSGGKTVMIDPGHGGSAPGNSSGGMIEKD
                     YNLNTSLATTEYLRSKGFNVIMTRDTDKTLSLGNRTALSNSLKPDLFTSIHYNGSTNK
                     QGHGVEVFYKLKDKNGGTTKTVATNILNRILEKFKLTNRGIKTRVLPSDSTKDYLYVL
                     RSNDMPAVLVECAFLDNENDMSLINSSAKVKEMGTQIGKGIEDSLK"
     gene            5340..>7073
                     /locus_tag="KR250_003572"
     CDS             5340..>7073
                     /locus_tag="KR250_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069452.1"
                     /translation="MEYLFEKSSLKEYGNEALDKYRELLDKTGNNSRCILLLVPNNNT
                     KIRYERALRLNYSEELKVTTYISFVKKELVKYWPLIIEKCDGISTKVVSPTFISNSLS
                     EYLIVQKVKEKRIQEGYFEDITGTNKSIANSIMTNINKSAFNLIDFDFIGEKIYSSKK
                     NKDSIYRFSYTQMDEIISYYINSLLSNSILDNALSIYLYNQYLLNDDFYLKNLFTEVR
                     YIIVDSLESSSNAEVDFITEALDNTLQSYIYFDYSRDYSVFNNVDIDYINEKIISKIK
                     PKEENIKKKEIRIEDLYLLPANIELNQSSQLYNEMLDLISEKVISLIEEDVSPRDIAI
                     ISPINNSILEHQIRDSLIEKNIDVFSTKKDKKAVDYPYGNALVVATCIFYGYLDFIKD
                     DEYLSFLETLLEINRIKAFKIFNETRHLSIDNIDEHSQYRDILQYIEEKKKSDIKIHE
                     FLTQFYIDKMLNLKEGKKNVGLCKKIIAESESFSESLSLLGMKKEKIFVEALKSTIND
                     YYSVVDIEELKSKDKIVITTPYSYISSNIDRSIQIWVDIGSNAWNMKIEKDISNLIVL
                     RKSFEEKKIYTN"
BASE COUNT         2946 a          795 c         1165 g         2167 t
ORIGIN      
        1 atttgatgtt cattcattag gatatatatg tagtttttag agtgctaact ctaaaaaatt
       61 agttttacta atttataaaa atttaatgcg ggtgtagctc aatggtagag ttctggcctt
      121 ccaagccagc tgtgagggtt cgatcccctt cacccgctcc aaaagattat gtggttacaa
      181 tagcagagag gatacacctg ttcccattcc gaacacagaa gttaagctct ctagcgctga
      241 tggtacttgg tgggaaactg cctgggagag taggacgtag ccacgtaatc tttttttgtt
      301 gtattaaatt taataaacct acaattgtta tatgttttta tttgtctttt atagattttt
      361 ttggcaataa atgatatagt gaagataatt aaatatattc aatatattta atttttttat
      421 tttttgtttt tataattttt ttagaagttt attttttatg tacttattta taattttggg
      481 taaaaacact ttgatataat ttcaaaaatt aaaaggttaa aaaacaaagt agaaatttat
      541 gttaaataag gagattaata tgagaagaaa tacaaaatta ttaacaacag ggattctttc
      601 aatggcaatc gtcgcaccta caatggcatt tgctactgaa tctaatgcta tggaaaataa
      661 cgctgattta aatataaact tagagaaaaa aagtatcgtt ttaggtagta cctcaaaagt
      721 cagtgttaag tttaaagaaa aaccagatgc agatagcatt acattaaagt ataaatgcta
      781 tgatatgcca ttggatacaa ctttaaacta caatcaatca actgagtcat atgaaggaac
      841 tatcaattat aataaagacc cagaatatct aaatgtttgg gaactacaag ggataacaat
      901 aaacagcaaa aataatccta aaactttaaa caaacaagaa ctagaaaaga tgggattaaa
      961 tttaaaagac tataatgtaa cacaggaatg tataattgaa gatataactt ctagaaaaga
     1021 tgtaaataaa tatttaagaa aaacttctgc acctattaca gaacttacag gaagtgatag
     1081 atacgaaaca gcagttaaaa taagtaaaga aggctggaaa aatggttcag ataaggtagt
     1141 tataataaat ggggatgtaa gtatagatgg cattatatca actccactag caactacata
     1201 taatgcgcca atacttttgg ttgaaaaaaa caatgtacct aatagtgtaa aatcagaatt
     1261 aaagcgttta aatcctaggg atgtaattat aattggagat gagaatgcta tttctaaaac
     1321 tactgctaat caaattaaat cgactgtaaa tgctagtcaa acacgtctaa agggctctaa
     1381 tagatatgag acatctttat tgatagcaaa ggaaatagat aaaaatcatg atgtggaaaa
     1441 agtgtacata acaaatgcta atggcggaga agtggatgca cttactatag cagcaaaagc
     1501 aggtcaagac aagcaaccaa ttatattaac tgataaaaat agtattacag acaacacata
     1561 taaatggtta aagagtgagg atttacaaaa tgcttatttt ataggtggcc ctcaaatgat
     1621 atcaacaaat gttataaata aggtaaatga tataactaaa gataatgtta ctaataatag
     1681 agtatatgga gcagatagac acgaaacaaa tgcaaacgta ataaaaaaat tctatacaga
     1741 tgatgagcta gaggctgttt tagtagctaa atcagatgta cttgttgatg ctttagcagc
     1801 aggtccattg gctgccaact taaaatctcc aatacttata acaccaaaga cgtatgtatc
     1861 tgcataccat aaagaaaact tagaagctaa atcggctaat aaggtatata aaataggagg
     1921 aggattgact tctaaggtaa tgaactctat agcatcatca ttatctaaac acaatacgac
     1981 tccaacagaa ccaggaaata gtgggggtaa gacagttatg attgacccag gacatggtgg
     2041 ttcggacact ggaacaacag gtaaaccatt agggggtatt aaagaaaaag actatacatt
     2101 aaatacttca cttgcgacaa ctgaatattt acgttcaaag ggattcaatg taataatgac
     2161 aagagataca gataagactt tatctcttgg aaatagaact gctttatcta actctttgag
     2221 accagattta tttacgagta tacactataa tgcatctgat acaactggga atggtgtaga
     2281 agtattttat aagcttaaag ataaagatgg aggaactact aagactgtag ctacaaatat
     2341 attaaataga atcttagaga aatttaatct aaaaaataga ggtgcaaaaa caagaacact
     2401 atcaactgac cctacaaaag attatttata tgttttaaga aataatgata tgccagctgt
     2461 acttgtagaa tgtgcatttt tggataatga gaaagatatg agtttactta atacatcaaa
     2521 taaagtaaaa gaaatgggta cacaaatagg taaaggcata gaagattcat taaaatagtc
     2581 tacaatgaga gcatagaaaa atttaaaata aattaatcta taattttaaa tttaaactaa
     2641 agctataaga taagaatgta attataatag taataaagac tgtatataca ttgtgcatat
     2701 aaatgcgaaa tgaggtatat acagtctttt tataattaat tgtaaaatga tttatgttat
     2761 atgaaaaatt tacatatatt aaggctacag ttaattgtca agagctggag tattcatttg
     2821 taatacacaa tataagctta attactatat tttattgtgt gttttttgtg gatttttatg
     2881 tcaataaatg atatagtata gataattaaa tgtattaaat atatttaatt tttttatttt
     2941 ttgagtttta taagtttatt tttatatatt acttatatat tacttatata ttatttatat
     3001 tttgggttag atacactttg acatattttc aaaagttaaa agattaaaaa taaagtagaa
     3061 atttatgtta agtaaggaga ttaatatgag aagaaataca aaattattaa caacagggat
     3121 tctttcaatg gcaatcgtcg cacctacaat ggcatttgct actgaatcta atgctatgga
     3181 aaataacgct gatttaaata taaacttaga gaaaaaaagt atcgttttag gtagcaaatc
     3241 aaaagttagt gtcaaattta aagaaaaacc agatgcagat agcattacat taaagtataa
     3301 atgctatgac atgccattgg atacaactct aaattacaat caatcaactg gggcatatga
     3361 aggaactatc aattataacc aagacccaga atatctaaat gtttgggaac tacaagggat
     3421 aacaataaac agcaaaaata atcctaaaac tttaaacaga caagacctag aaaagctggg
     3481 attaaattta aaagactata atgtaacaca ggaatgtata attgaagaca taacttctag
     3541 aaaagatgta aataaatatt tgagaaaaac ttcttcacct attacagaac ttacaggaag
     3601 tgatagatat gaaacagcag ttaaaataag taaagagggt tggaaaaatg gttcagataa
     3661 agtagttata ataaatgggg atgtaagtat agatggcatt atatcaactc cactggcaac
     3721 cacatataat gctccaatac ttttggttga aaaaaacaat gtgcctaata gtgtaaaatc
     3781 agaattaaag cgcctaaacc ctaaagatat aattataatt ggagatgaga atgctatttc
     3841 taaaactact gctaatcaaa ttaaatcaac tgtaaatgct agtcaaacac gtttaaatgg
     3901 ttctaataga tatgagacat ctttattgat agcaaaggaa atagataaaa atcatgatgt
     3961 ggaaaaaata tacataacaa atgctaatgg cggagaagtg gatgcactta ctatagcagc
     4021 aaaagcaggt caagacaagc aaccaattat attaactgat aaagatagta ttacagacaa
     4081 tacatataaa tggttaaaga gtgaggattt acaaaatgct tattttatag gtggtcctca
     4141 aatgatatca acaaatgtta taaataaggt aaatggaata actaaggata gtgttactaa
     4201 taatagagta tacggagcag atagacacga aacaaatgca aacgtaataa aaaaattcta
     4261 tacagatgat gagttagagg ctgttttagt agctaaatca gatgtacttg ttgatgcttt
     4321 agcagcaggt ccattggctg cgaacttaaa atctccaata cttataacac caaagacgta
     4381 tgtatctgca taccataaag ataatttaga agctaaatca gctaataaag tatacaaaat
     4441 aggaggagga ttgacttcta aggtaatgag ctctatagca tcatcattat ctaaacacaa
     4501 tacgactcca acagaaccag gaaatagtgg gggcaagaca gttatgattg acccagggca
     4561 tggtggttca gcacctggaa attcatctgg aggaatgatt gaaaaagatt acaatttaaa
     4621 tacttcactt gcaacaactg aatatttacg ttcaaaggga ttcaatgtaa taatgacaag
     4681 agacacagat aagactttat ctcttggaaa tagaactgct ctatctaatt cattgaaacc
     4741 agatttattt acaagtatac attataatgg ctcaactaat aaacaaggtc atggtgtaga
     4801 agtattttat aagcttaaag ataaaaatgg agggactact aaaactgtag ctaccaatat
     4861 attaaataga attttagaga aatttaaact tacaaataga ggtataaaaa caagagtact
     4921 tcctagtgat tctacaaaag attatttata cgttttaaga agtaatgata tgccagctgt
     4981 acttgtagaa tgtgcatttt tggataatga aaatgatatg agtttaataa actcatctgc
     5041 aaaagtaaaa gaaatgggta cacaaatagg taaaggaata gaagattcat taaaataaat
     5101 ggaaataaaa atatttaaaa acttaatgta aatttatagt tgtaaattta tactaaagtc
     5161 aaaaaatgaa atataagtgt atcaatagta agactgtatg tacatttgca catgtgaatg
     5221 caagtatata tacagtcttt ttttgtagtt aaatgcaata atttgattgt gttttgtaaa
     5281 atacacaata tgatataatt agataatata cacttaattt aattgaggaa ggtgattata
     5341 tggaatattt atttgaaaaa agtagtttaa aagaatatgg aaatgaagct ttagataaat
     5401 atagagaatt attagataaa actggtaata attcaaggtg tattcttttg ttagtaccaa
     5461 acaataacac aaagatacga tatgaaaggg ctctaagatt aaattatagt gaagaactga
     5521 aagtaacaac atatataagt tttgtgaaga aagaattagt taaatactgg ccactaataa
     5581 tagaaaaatg tgatggaata agtacaaagg ttgtatctcc aacatttata tcaaatagtt
     5641 taagtgaata tttaatagtg caaaaagtta aggaaaaaag gatacaagaa ggatattttg
     5701 aagatattac aggaaccaat aaatccatag caaatagtat aatgactaac ataaataagt
     5761 ctgcatttaa tttaattgat tttgatttta ttggagaaaa aatatattca tcaaagaaaa
     5821 ataaagatag tatatataga ttttcataca ctcaaatgga tgaaattata tcatattata
     5881 taaattcctt actatcaaat tcaattttag ataatgcatt aagcatatat ttatacaatc
     5941 aatacctact taatgatgat ttttatttaa aaaacttatt tacagaagtg agatatataa
     6001 ttgtggatag ccttgaaagc tcaagtaatg cagaagttga ttttataaca gaagcattgg
     6061 ataatacatt acaaagttat atatattttg attatagtag ggattattct gtatttaata
     6121 atgtggatat agactatata aatgagaaaa taatatcaaa aattaagcct aaagaagaaa
     6181 atattaaaaa gaaggagatt agaatagaag atttatactt attacctgca aatatagaat
     6241 taaatcaatc aagtcaatta tataatgaaa tgttagattt aataagtgaa aaagttatat
     6301 ctcttataga agaagatgta tctccaagag atatagcaat tatatctcct ataaacaata
     6361 gtatattgga gcatcaaatt agagattctt tgatagaaaa aaatatagat gtttttagta
     6421 ctaagaagga taaaaaagct gtggattatc catatgggaa tgcattggta gtggctacat
     6481 gtatattcta tggatattta gattttataa aagatgatga atacttaagc tttttagaaa
     6541 ctcttttaga aataaatagg ataaaagcat ttaagatttt taatgaaaca agacatttaa
     6601 gtattgataa tatagatgaa catagtcagt atcgtgatat tttacagtat attgaagaaa
     6661 aaaagaagag tgatataaaa atacatgaat ttttaactca attttacata gataaaatgt
     6721 taaatttaaa agaaggtaaa aagaatgttg ggttatgtaa aaagataata gctgaaagtg
     6781 agtcttttag tgaaagttta agtctacttg ggatgaaaaa agaaaagata tttgtagaag
     6841 ctttaaaaag tacaataaac gattattact ctgttgtcga tatagaagag ttgaaatcca
     6901 aagacaaaat agtaataact actccttatt catatatatc atcaaatata gatagaagta
     6961 tacaaatatg ggtagatata ggaagtaatg cttggaatat gaaaatagaa aaagatataa
     7021 gtaatttaat tgtacttaga aaatcttttg aagaaaaaaa gatttataca aat
//
LOCUS       DAEPIN010000127         6990 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.129, whole genome shotgun sequence.
ACCESSION   DAEPIN010000127 DAEPIN010000000
VERSION     DAEPIN010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6990
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.129"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            <1..332
                     /locus_tag="KR250_003573"
     CDS             <1..332
                     /locus_tag="KR250_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBH0069453.1"
                     /translation="DIICIEDLQVKNMIKNHKLSRLISDVSWSEFIRQLEYKANWYGR
                     QIVKVGKFFASSQICNKCGYKNEEVKNLNIREWICPSCNETHDRDINASINILKEGLR
                     LITIQNK"
     gene            459..1628
                     /locus_tag="KR250_003574"
     CDS             459..1628
                     /locus_tag="KR250_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HBH0069454.1"
                     /translation="MNELCKDEVVEFCNRIKGKVESVAISGVFAPVNKQQEEEVAKWV
                     REILGDIPISLSNEIGNIGILERENGAILNSALSQVGKAVSLGFEKALKDLGIDAQIY
                     FSQNDGTLMNLEYTMKYPIFTIGCGITNSIRGASFLSKVKNAIVLDVGGTTSDLGVLY
                     NGFPRESSIPVTIGGVHTNFRMPDVLTIGLAGGTCVKGEKDNIKIGPESVGYRITEDA
                     IIFGGDTLTLSDVVTKLDMAFEDRKSNVENLDKEYCNEVYKKVILKLEDAVDQMKTSQ
                     DDVELVLTGGGSIIVPEKLKGISNVIRPPHYTAANAIGAALGQISGDVEKVYSLDKMS
                     REDAIEDAKKEAVNKALKAGAKEDTIEVLNIEDVPLAYLPGNALLIKVKVVGDLI"
     gene            complement(1791..2333)
                     /locus_tag="KR250_003575"
     CDS             complement(1791..2333)
                     /locus_tag="KR250_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HBH0069455.1"
                     /translation="MKSYIALLRGINISGKNKIIMSELKASFVELGYSEVSTYLNSGN
                     VVFLSDVDDSKVISNTIRLMIKKQFELEIPVFIISQEELKDILNNAPNWWGDNNKEIY
                     DNLIFMFPDLSYDEFYDEVGNPKEEYEKVYHYKNAIFWSFSRKDYKKTNWWSKTASSN
                     VSDKITIRTANTVRKIVGMR"
     gene            complement(2481..2765)
                     /locus_tag="KR250_003576"
     CDS             complement(2481..2765)
                     /locus_tag="KR250_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBH0069456.1"
                     /translation="MAKNTKQTTVHIDETLLKEVKRIGINENKSISQIINESIIDYIL
                     VYRENEDKEKNEHDKNLNETINEFKKEEEKRMQAKSAARAIAEAKKRVLY"
     gene            complement(2828..3592)
                     /locus_tag="KR250_003577"
     CDS             complement(2828..3592)
                     /locus_tag="KR250_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HBH0069457.1"
                     /translation="MNNYLKFATYQLIGFTSSIPRLIKIKKNPDKFSLKEKFEFMQKQ
                     AKKSLDIVNIELNIIGKETLPKEPLLFVVNHSSMLDSFILTASVERPIGCVIADEPVW
                     RNIPIFKEWAKLLRCVYVNRKNNREGIKSIAQASQNILTGQSMAVFPEGDLTWIKEPN
                     SLVSEFRSGALKIAYKAKCPIVPLVIKNSKDTYEGYQPIGKINSVPVEVEFLEPIYDH
                     IENPRLKSSVLGENIKNKMINTIENFRKSNKTFKEF"
     gene            complement(3874..4140)
                     /locus_tag="KR250_003578"
     CDS             complement(3874..4140)
                     /locus_tag="KR250_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HBH0069458.1"
                     /translation="MIESRCGILCYECGYKEKVNCKGCTEIEKPFWGEQCPVKSCCED
                     KNLTHCGLCDTFPCEMLNQFAYDKEQGDDGKRISQCKKWAVLAL"
     gene            complement(4182..4991)
                     /locus_tag="KR250_003579"
     CDS             complement(4182..4991)
                     /locus_tag="KR250_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0069459.1"
                     /translation="MNKIINNILNTCKQYTYILPHKLLRQYIAHYTISIPDTSIKENL
                     TLIPDASGCMIFKFDKKGIESAFWGATTKTTIVKNDIENVLFRVFVEFRPGGVYYLTG
                     LSQRESTDLKISLEDFNTLFSLEVNSIFERTSTIEELVEQLDMLFLSYLLKSNIVDMT
                     IPILENARKQNSIMSVKNISQISCYSERHLNRIFNNSLGMSVKSYLRLLRINLVLQEI
                     QNNKIPFATLAQDIGYYDQSHFINDFKSICGVNPTTYIKNLSDFYNEKYKF"
     gene            5304..5585
                     /locus_tag="KR250_003580"
     CDS             5304..5585
                     /locus_tag="KR250_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069460.1"
                     /translation="MASKQTPRNNLEKFLRIRVLSHTGDKIHIKLPVNFAKRMIENNA
                     LDLFNGKDDVVDGKKVTEILVKAFDYDLTGEVVHLERKNGDIIKVSIVQ"
     gene            5849..6103
                     /locus_tag="KR250_003581"
     CDS             5849..6103
                     /locus_tag="KR250_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069461.1"
                     /translation="MENKDFKLTICIFAFVIGVVVLFCCIGLGEFSAEHIIQQNGGIM
                     DTDQYNIYLDQSISQYRNFGSILALLGGIGILFDKYIRKY"
     gene            6148..6471
                     /locus_tag="KR250_003582"
     CDS             6148..6471
                     /locus_tag="KR250_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069462.1"
                     /translation="MATEIFTVKITPLEAQKMIKENLDADLVFSDSYNLGDDKFILIT
                     TFEKYYARNNSDAGLIVVYENTTGKTVIKATSTGSAIGILKIDWGAGGNLLKRVKNIL
                     ADYII"
BASE COUNT         2583 a          825 c          999 g         2583 t
ORIGIN      
        1 atgatattat ttgcatagaa gatttacaag taaagaatat gattaaaaat cataaactat
       61 ctcgcttaat ttcagatgta tcatggtcag aatttattcg tcaattagaa tacaaggcta
      121 attggtatgg tagacaaatt gtaaaggtag gtaaattctt tgcaagttca caaatatgca
      181 ataaatgtgg gtataaaaac gaggaagtta agaacttaaa tataagagaa tggatttgtc
      241 ctagttgtaa tgaaactcac gatagagata taaatgcaag tataaatata ttaaaagaag
      301 gattaagact aataacaatt caaaataaat aacgtatgta agaaccggag gaactacggg
      361 gatagcctgt ggagaattag taagacatat tttagtatgc aaaattctgt gaagcaggaa
      421 ccctgtgact ttagtcatgg gaggttcagg gtagaaagat aaatgaactt tgtaaagatg
      481 aagttgtaga gttttgcaat agaataaaag gtaaagtgga aagtgttgca ataagtggag
      541 tatttgctcc agtaaataaa caacaagaag aagaagttgc aaaatgggtt agagaaattt
      601 taggggatat tcctatatct ttatcaaatg aaataggtaa tataggtata ttagaaagag
      661 aaaatggagc tattttaaat tctgcattgt ctcaagtagg taaagctgta tctttaggat
      721 ttgaaaaagc acttaaagac ttaggtatag atgctcaaat atatttcagt caaaatgatg
      781 gaacattaat gaatttagag tatacaatga aatacccaat ttttacaatt ggatgtggaa
      841 taacaaattc tataagagga gcttccttct tatcaaaagt aaaaaatgca atagtattag
      901 atgttggtgg aactacaagc gacttgggtg tactatataa tggattccct agagaatcat
      961 ctattccagt tacaattgga ggagtacata caaactttag aatgccagat gttttaacta
     1021 ttggtcttgc tggtggtaca tgtgtaaaag gcgaaaaaga taatataaag ataggtcctg
     1081 aaagtgtggg atatagaata actgaagatg caataatatt tggtggagac actctaactt
     1141 tatcagatgt tgttacaaaa ttagatatgg cgtttgaaga tagaaaaagc aatgttgaaa
     1201 acttagataa agagtattgt aatgaggtat ataagaaggt tattttaaaa ttagaagatg
     1261 ctgtagacca aatgaagact agtcaagatg atgttgaatt ggttttaact ggtggaggaa
     1321 gtataattgt tcctgaaaaa ctaaaaggca taagcaatgt tataagacct cctcattata
     1381 ctgctgcaaa tgctattggt gcagctttag gacaaataag tggagatgta gaaaaagtat
     1441 acagtttaga taaaatgtcg agagaagatg ccatagaaga tgcaaaaaaa gaggctgtaa
     1501 ataaagctct aaaagctggt gctaaagaag atactataga agtattaaat atagaagatg
     1561 ttccacttgc ataccttcct ggtaatgcac ttcttataaa agtaaaagtt gttggtgatt
     1621 taatttaaag aataaatata agtatttcca gaattatata aatatttctg gactcatttt
     1681 taaacttatt ttatcaataa aaacagcata acttttgact atattcttat aagtttccga
     1741 acagccgata tatacctatt ttttgaggtt tatcggctgt ttgttatctt ttatcgcatt
     1801 ccaactattt ttcttaccgt atttgctgta cgaattgtaa tcttatcact tacatttgaa
     1861 ctggcagtct ttgaccacca atttgtcttt ttataatctt tacgactaaa agaccaaaag
     1921 atagcatttt tataatgata tactttttca tactcttctt taggatttcc aacctcatca
     1981 taaaactcgt cataagataa gtcaggaaac ataaaaataa gattatcata tatttctttg
     2041 ttgttatctc cccaccaatt aggagcatta tttagtatat cttttaactc ttcttgtgaa
     2101 ataataaaaa ctggtatttc caattcaaac tgttttttta tcattaatct tattgtattt
     2161 gagataacct tgctgtcatc tacatcactt aaaaagacaa cattaccact attaaggtat
     2221 gtagaaactt ctgaataacc aagttctaca aagcttgctt ttaattcaga cataattatc
     2281 ttgttcttac cacttatatt gatacctctt aataatgcga tataactttt catataataa
     2341 tttcacctct aaacattcac atttttctat gtagtacaaa ctggaatttg catcttttat
     2401 aattctttca aaacttcttc atatgtaatt tttttaatat aaaaaaatcg gtgcttaatt
     2461 tgtaccgatt tttgtttttc ttagtaaaga actcgttttt ttgcttctgc tatagctcta
     2521 gcagcacttt ttgcttgcat tcttttttct tcttcttttt taaattcatt tattgtttca
     2581 tttagattct tatcatgttc atttttttct ttatcttcgt tttctctgta tactaatata
     2641 taatctatta ttgattcgtt tattatttga gatatacttt tattttcatt aattcctatt
     2701 ctcttaactt ccttaagtag agtctcatca atatgaaccg ttgtttgctt tgtatttttt
     2761 gccaccttaa tcacctctta aaaaaaattc taacattaaa atttttttta gtcaataaat
     2821 tttaatatta aaattcttta aatgttttat ttgattttct aaaattttct attgtattta
     2881 tcatcttatt ttttatattt tcacctagta cagaagactt tagtctagga ttttcgatat
     2941 ggtcataaat tggttctaaa aattcgactt cgacaggaac agagttaatt ttaccaatag
     3001 gttggtatcc ttcataagta tcttttgaat tttttattac tagtggaaca atagggcatt
     3061 ttgctttata tgctatcttt aaagctccac ttctaaactc cgaaaccaat gaatttggtt
     3121 ctttaatcca tgttaaatct ccttctggaa acactgccat actttgacct gtaagtatat
     3181 tttgagaggc ttgggctata ctctttatac cctcacgatt gttttttctg tttacatata
     3241 cacatcttag taattttgcc cattctttaa atataggtat atttctccaa actggttcat
     3301 ctgcaattac acatccaatt ggtctttcta cacttgctgt tagtatgaag ctgtcaagca
     3361 tactagaatg atttacaaca aatagaagtg gttcttttgg cagtgtttcc ttaccaatta
     3421 tattaagctc tatgttcact atatctaaag attttttagc ttgtttttgc ataaattcaa
     3481 atttttcctt taagctaaat ttatcaggat tctttttaat tttaataagc ctaggtatag
     3541 aagatgtaaa acctataagt tgatatgtag caaattttaa gtaattattc ataaaattat
     3601 ctccttaaaa taaaattatt tattactgta aagattttaa cataaaataa aatttaagtc
     3661 tattttaatt caattttgta acatatattt taaatagaca gagattgaga ttttatattt
     3721 ccaattttag taatcagatt ttatcaataa attactattc tattaattta attaaataac
     3781 ctttaaattt aattataaaa actgttgcag aaatttctac aacagtttaa aaaatttacc
     3841 ttacaaaaac ttattttata acaagagagt atattataag gctagtacag cccatttttt
     3901 gcactgagaa attcttttgc catcatcacc ttgttctttg tcatatgcaa attgattaag
     3961 catttcacat ggaaatgtat cacatagacc acaatgtgta aggtttttat cttcacaaca
     4021 agattttaca ggacattgtt caccccaaaa tggtttctca atctctgtac aacctttaca
     4081 attgactttt tccttgtatc cacattcata acataaaatt ccacatcttg attctatcat
     4141 tttaagtcac tccttaaata taaattttat atgattgtat atcaaaattt atacttttca
     4201 ttgtaaaaat cggacaaatt ttttatatag gttgttggat taacaccaca aatagattta
     4261 aaatcattta taaaatgaga ttggtcgtaa taaccaatat cttgtgctaa agttgcaaat
     4321 ggtattttat tattttgtat ttcttgtaat acaaggttta tacgaagtaa tctaaggtat
     4381 gatttaacac tcataccaag agaattatta aaaattctat ttaaatgtcg ttcactataa
     4441 caactaattt gtgaaatgtt tttaacagac ataatagagt tttgttttct agcattttca
     4501 agaattggta tagtcatatc tactatattt gatttcaata agtatgataa gaataacata
     4561 tctaattgtt ctactaattc ttctatagta cttgttcttt caaaaatgga attaacctct
     4621 aagctaaata atgtgttaaa atcttcaagt gaaattttta aatctgtaga ttctctttga
     4681 gaaagacctg tcaaataata tactccacct gggcgaaact caacgaatac tctaaaaagt
     4741 acattttcta tatcattttt tacaatagta gtttttgttg tggctcccca gaatgcgctt
     4801 tctatacctt ttttgtcaaa tttaaatatc atacatccac tagcatcagg tattaatgta
     4861 agattttctt taatacttgt atctggaata gatattgtat agtgtgctat atactgtctt
     4921 aacaatttat gtggcaaaat atatgtatac tgtttgcagg tatttaaaat gttattgatt
     4981 attttattca taagtatatc ctcctgatat ataaaaaata agtatactaa tttatttttt
     5041 atataataaa ccagttttgt tttaattgaa atattctaat aagatatttt gttaaattta
     5101 gatacaataa ttgtaaagta aaatctcaaa atatacaatt attataaatc atagatattt
     5161 attaaataaa tatattaaag aagaaggaat ataaaacaag attagaaaat aatagtgtta
     5221 ctttcataat aaatacatat aaagatattt ttaagaataa aatttattat taaataaaaa
     5281 ataaaggaga gtgatttaat agtatggcat caaaacaaac accaagaaac aatttagaaa
     5341 agtttttaag aataagagta ttatcacata caggcgataa aatacatata aagttacctg
     5401 taaattttgc aaagagaatg attgagaata atgccttgga tttatttaat ggaaaagatg
     5461 atgtagtaga tggtaaaaaa gttactgaaa tattagttaa ggcatttgac tatgatttaa
     5521 ctggagaagt agttcacttg gagagaaaaa atggagacat tattaaagtt tcaattgtac
     5581 aatagaatta ttttatatga attttatttt tgaatagtta taaataatta gaaatttgta
     5641 aaaaagtata atatattatt agtaatggta cagaatacac atttataatt tatagatgtg
     5701 tattttttgt ttaaaaaatt taaatatata aaactataaa ggttacaatt ttggaaatag
     5761 atgtaaatat atgttgtatt gtataataag agtaatataa aatctagtaa aaataatcaa
     5821 ataaatttag cttatgagag ggataactat ggaaaataag gattttaaat taacaatatg
     5881 tatatttgct tttgtaattg gagtagtagt attattctgt tgtataggtt taggtgaatt
     5941 tagtgcagag catattattc aacaaaatgg tggaatcatg gatactgacc aatataatat
     6001 ttatctagac caatctattt ctcaatatag aaattttggt tctatattag ctctgttagg
     6061 aggaataggg atattgtttg ataaatatat cagaaaatac tagagattat gttttgtcta
     6121 aaataaattc ttatgaggag ataaaaaatg gcaacagaaa tatttactgt aaaaattaca
     6181 cccttagagg cacaaaaaat gataaaagaa aatcttgatg cagatttagt ttttagtgat
     6241 tcttataatt taggagatga taaatttata ctaattacaa cttttgagaa gtattatgca
     6301 agaaataata gtgatgcagg attaatagta gtttatgaaa ataccacagg aaaaacagtt
     6361 attaaagcta cttcaacagg cagtgcaatt ggaattttaa aaattgattg gggtgcaggt
     6421 ggaaatttac ttaaaagagt aaaaaacata cttgctgatt atattattta attattttat
     6481 ctaactgata atatgtcaag aaaatctcgt aaaagaggtt aagaacattt ttataatata
     6541 tagataaata agaatttgaa ggtaaagttt ttaaggtata aagaaaaata atataataaa
     6601 acgaattggc tttataattc tttataaggc aaagagcaca tttctttata taattgttgg
     6661 atattaaata atatgtaaat acaccattta catataaaaa tttaattaat atgttaagag
     6721 aaaatgaaat ggaaattact aaaaaatatt tttaaattgg ataatggttg atattatgtt
     6781 tatacaaaaa tcatgtggaa ttttatatat tttaaatatg atagtttaaa attatgaaag
     6841 aatgataaat tccagtttgt agggtaattt tgataaataa tattaataag tatgagtaaa
     6901 aagtaggagc atattaatat aatgtcccta cttttttatt attaaaattg tttataatat
     6961 caacactaca ttaaaacaca acctaaattt
//
LOCUS       DAEPIN010000128         6911 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.130, whole genome shotgun sequence.
ACCESSION   DAEPIN010000128 DAEPIN010000000
VERSION     DAEPIN010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6911
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.130"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            130..1119
                     /locus_tag="KR250_003583"
     CDS             130..1119
                     /locus_tag="KR250_003583"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HBH0069463.1"
                     /translation="MFENLPKIDLHCHLDGSVRVETMLDIAIKEKIDLPSNNMDEIKK
                     LAKVSFNCTSLDEYLEKFDLPLKVMQSKENLKRITFELLEDASRENVKYIEIRFAPLL
                     HTQKGMSVKNIIEGIIEGIREAESIYDIKGNLILGCMRTMTSKEALLVIEEGKSFVNK
                     GVVAVDLCGPEKEGFCKEYKDVFKLAREYGYKVTIHAGEAASGENVLDAINILKADRI
                     GHGVKIKDHKKAYNLVKDKKILLELCPTSNVQTKTVDSYEAHPFYTFYKDNLHVSINT
                     DNRTVSDINLSSELNVIFDTFKLGLEDYKIIYRNAVEASFADKETKEYLNSLI"
     gene            1191..1931
                     /locus_tag="KR250_003584"
     CDS             1191..1931
                     /locus_tag="KR250_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EcsC family protein"
                     /protein_id="HBH0069464.1"
                     /translation="MKDYEDVVYIKLVKKELEEWKKNILKKPSIFDKASKGIQNKFNG
                     VLPDKYHEIITSSIKVMTKTVLFGYKYATKPPLRNMSLEEREKLVLEKAKTYKTTAMI
                     EGAGTGAGGILLGLSDFPLLLGIKVKFLYDVASIYGYDVKDYRERIYILNILQLAFSS
                     QQHINKTFKLMNNWDEYVNSLPEDINDFDWRTFQQEYRDYLDLAKFLQLMPGIGAFVG
                     FYVNGKLIDKLCETAVSAYRLRIRELKL"
     gene            2147..3298
                     /locus_tag="KR250_003585"
     CDS             2147..3298
                     /locus_tag="KR250_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HBH0069465.1"
                     /translation="MTNLPKQFDSFNEARQKGFINAKELKESGKKMVGVFCTFTPVEI
                     PMAAGATVVGVCGVSEEPIPDAERVLPRNLCPLIKSSYGHAITDTCPYFYFSDLLIGE
                     TTCDGKKKMYEELAKVKPTYVMHLPNTSKGEFAYKLWKDEMIRLKEEVEKSLGVTITE
                     EDIRTAIKDKNEERELLKEFYALGKLQPSALTGLELHNVLYQAGFKFDRAELKHSLRK
                     VIDDMKERYEKGECPVQKDKPRILITGSPIGGISEKIVKTLEDAGASVVAYELCGAIR
                     SNDLLVDEEIEDVYDALTQKYINIGCSCMMNNDNRIELLDRIIDEYNVDAVIDVVLQA
                     CHTFNIESYRIREFVTKEKNKPFMSLETDYSKSDTEQLRTRFEAFVEML"
     gene            3323..4078
                     /locus_tag="KR250_003586"
     CDS             3323..4078
                     /locus_tag="KR250_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyglutaryl-CoA dehydratase"
                     /protein_id="HBH0069466.1"
                     /translation="MFSIGVDSGSVATKGVLFDGEKIIKKIIIPTGWSPKSTSKQVYE
                     LLSSEIDKKDIKKVVGTGYGRGVMDFADKKVTEITCHTRGIYFLNKNIRTILDVGGQD
                     SKVINLDRDGNVFNFIMNDKCAAGTGRFLEITSNLLGSDIESIDTLAKGYEPVNISSM
                     CTVFAESEIVSLLAQNISTGEVAAGILKSIANKSTSMLARGEVIDEVAFTGGLAKSKE
                     LVKMIEEILGKKIFIAEDTQIIGALGAAVIGFR"
     gene            4270..6657
                     /locus_tag="KR250_003587"
     CDS             4270..6657
                     /locus_tag="KR250_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding cysteine protease Cwp13"
                     /protein_id="HBH0069467.1"
                     /translation="MKKFTSKKVTRFVALFLISILVMSTIPVSADNSNTLNQIKKAEY
                     SETYKQYLNDAKNGCTEKYNGIIPNPYQLEGTQIQSKGRTAPTSYDPRKLGLMTSIKN
                     QEDLGICWDFAAMATLESFLKLNNYGDYDLSEEHLRWWASDGEYGWSVNDMNGALNYE
                     AMGYLTSWSGPKLEKDIPYNGRVSKAQGAKKPTNMNTAPTVFNVTDAVCVSNDINSTK
                     NAILQYGAVTSGYYEDIKYQSDDQNSYYCPTKSFNTNHAISIVGWDDNYSKDNFNANI
                     RPSKNGAWLIKNSWGDYNSEGGYFWISYEDKTLMSDIDNFSIKGGKKPNDDEKMYQHD
                     YASIVPLISKKITAANVFDFNRGDETLKSVMFLTESIGAKYEVYYAPVVNGIPQENNM
                     KKLKEGTAQYSGYITVPIDSFDIPEGKGAIVVSIEGKNGESTIGSESNVPGYDTFKAK
                     ANLGESYIIDNTGKFFDINRDSNFYPCNFTIKAVTEKSSGESIPNESLIGSDRYETAI
                     KVSQNGFNSSENVVLVNGSSIVDALAATPFTSAINSPILLTQKEALNSKTKAEIQRLG
                     AKKVYLIGGENSISKEIEQQLKSLNISIERISGSDRYKTSLLLAQKLNGIKNVSQIAV
                     VNGVKGLADAISVGAAAAENNIPIILANEKSELQGADEFLNSLNIEKSYIIGGTASLS
                     NNLESKLKNPTRLSGSSRDETNSKIIDNFYKKDTLKNAFVVKNGIKNQNDLIDGLAVG
                     PLGAKTGSPVILVGDKLADSQKEVLKNKTLEKVTQVGGGANKNAFKELVKLKSAK"
BASE COUNT         2797 a          705 c         1273 g         2136 t
ORIGIN      
        1 aaattaaatt aaaatatttt aatgagatat gactttttta gaattaaaaa ccgtagtata
       61 ttgatataat agaaataaga cttaaaaatc tgttgatagt attatttata agtacagtag
      121 gaggataaaa tgtttgaaaa tttacctaag atagatttac attgccactt agatggaagt
      181 gttagagtag aaaccatgct tgatatagct attaaagaga aaatagattt accttcaaat
      241 aatatggatg aaataaaaaa attagcaaag gtttctttta attgtacttc actagatgaa
      301 tatttagaaa aatttgattt acccttaaaa gtaatgcaat ctaaggaaaa tttaaaaaga
      361 attacttttg aacttttgga agatgcaagt agagaaaatg taaaatatat tgaaattaga
      421 tttgcaccat tacttcacac ccaaaaaggt atgagtgtta aaaatataat tgagggtata
      481 attgaaggaa ttagagaagc tgaaagtatt tatgatataa aaggcaattt aatattaggt
      541 tgtatgagaa caatgacctc aaaagaggca ttattagtaa ttgaagaagg gaaatcattt
      601 gtaaataaag gtgttgtagc agtagacttg tgtggtccag agaaagaggg attttgtaag
      661 gaatataaag atgtttttaa attagcaaga gaatatggat ataaagttac aatacatgca
      721 ggagaagctg caagtggaga aaatgtattg gatgctataa atattttaaa ggcggatagg
      781 attggtcatg gggtaaaaat taaagaccat aaaaaggcgt ataatttagt taaagataaa
      841 aaaatattgc ttgaattatg tccaactagt aatgtacaaa ctaagacagt agattcttat
      901 gaagcacatc cattttatac tttttataaa gataatttac atgtaagtat aaatactgat
      961 aatagaactg tatcagatat aaatttaagt tcagaactaa atgttatatt tgatactttt
     1021 aaactaggat tagaagacta taaaataatt tatagaaatg cagtagaagc tagttttgca
     1081 gataaagaaa ctaaagagta tttgaatagt ttaatttaat agataaaaat atttaaactt
     1141 taaattaagc tattaaattg ataaaaatac attgtattag gagaaaaaat gtgaaagatt
     1201 atgaagatgt tgtttatatt aaactagtca aaaaagaact tgaagaatgg aagaaaaata
     1261 tattaaagaa accttctata tttgacaaag catcaaaggg tatacagaat aaatttaatg
     1321 gagtattgcc agataaatat catgaaataa ttacttcttc tataaaagta atgacaaaaa
     1381 ccgtactgtt tggatataaa tatgctacaa agccaccact tagaaatatg tcattagaag
     1441 aaagagaaaa attagtttta gaaaaagcta aaacttacaa gactacagca atgattgaag
     1501 gggcaggaac aggggcagga ggaatcctct taggtttatc tgattttcct ttgcttttag
     1561 gtattaaagt gaaattttta tatgatgtag cttcaatata tggatatgat gttaaagatt
     1621 atagagaaag gatttatata ttaaatatat tacagttggc tttttctagt caacagcata
     1681 taaacaaaac ttttaaattg atgaataact gggatgaata tgtaaatagt ctaccagaag
     1741 acatcaatga ttttgattgg agaacatttc aacaagagta tagggattat ttagatttag
     1801 ctaagttttt acaactaatg cctggaatag gagcatttgt aggattttat gtgaatggaa
     1861 aattaattga taaattatgt gaaactgctg tgtctgctta tagattgagg attcgagagc
     1921 ttaagttata agaaaaatta taacataaaa aattagttat acaatatatc tatatgtatc
     1981 taattttgca ttgataaaaa caattatgga taaaaggtaa agcatgatag aatctaatgt
     2041 agaggtttgt attttaatgg aaaaatttag tttttattgc ttaagataca aattgaatta
     2101 cgaagagatt gaattatgaa gagaagtaat tgggaggaat attttcatga caaatttgcc
     2161 aaaacaattt gatagcttca atgaagctag acaaaaagga tttataaatg caaaggaact
     2221 taaggaaagt gggaaaaaga tggttggagt tttctgtaca tttactccag ttgaaattcc
     2281 aatggcagct ggagcaactg tagtaggtgt atgcggcgtt agtgaagagc caatacctga
     2341 tgcagaaagg gtgcttccaa gaaatctttg tcctttaatt aaatcaagct atggacatgc
     2401 aataacagat acctgtcctt atttctattt ttctgatttg ttgataggag aaaccacttg
     2461 tgatggtaag aaaaaaatgt atgaagaact tgcaaaagtt aagccaacat atgtaatgca
     2521 tttgccaaat acttcaaaag gagaatttgc ctataaactt tggaaagatg aaatgataag
     2581 attaaaagaa gaggttgaga aatctttagg tgtaactata actgaagaag atataagaac
     2641 tgctataaaa gataaaaatg aagaaagaga acttttaaaa gagttttatg cacttggtaa
     2701 attacaacca tctgcactta caggccttga gttacataat gttttatatc aagcaggttt
     2761 taaatttgac cgtgcagaac ttaaacattc tttaagaaaa gttatagatg atatgaaaga
     2821 aagatatgaa aaaggtgaat gtcctgttca aaaagataag ccaagaatat tgataacagg
     2881 ttctcctatt ggtggaataa gcgaaaaaat agttaaaact ttagaagatg caggggcatc
     2941 agtcgttgca tatgaacttt gtggagctat tagatcaaac gatttattgg ttgatgaaga
     3001 aatagaagat gtatatgatg ctttaacaca aaaatatata aatataggtt gttcttgtat
     3061 gatgaataat gacaatcgta ttgaattatt agatagaata atagatgaat ataatgtaga
     3121 tgctgtaata gatgtagttt tacaagcatg tcatacattt aatatagaaa gttatagaat
     3181 aagagaattt gttacaaaag aaaaaaataa gccatttatg tctcttgaaa ctgattattc
     3241 aaaatcagat actgaacaat taagaactag atttgaagcg tttgtagaga tgttgtagga
     3301 ataaataaaa gtaggaggaa ctatgtttag tataggagtt gactcagggt cagtggcaac
     3361 aaagggtgtg ctatttgatg gtgaaaaaat tattaagaaa ataattatac caactggatg
     3421 gagccctaaa agtacatcta aacaggtcta tgagttgcta agtagtgaaa ttgacaaaaa
     3481 agatataaaa aaggtggtag gtacaggtta tggaagggga gtaatggatt ttgctgataa
     3541 gaaagtaaca gaaataactt gtcatactag aggtatttac tttttaaaca agaacataag
     3601 gactatttta gatgtaggtg gtcaagatag taaagttata aatttagata gagatggaaa
     3661 tgtatttaac tttataatga atgataagtg tgctgcagga actggtcgat ttttagagat
     3721 tacttctaac cttttaggaa gcgatattga aagtatagat acattagcta aaggatatga
     3781 accagttaat atatctagta tgtgtacagt ttttgctgag tctgaaattg taagtcttct
     3841 agctcaaaac atttctactg gagaggtagc agcagggata cttaaatcta ttgcaaataa
     3901 atccacctca atgttagcaa gaggtgaggt aatagatgaa gttgcattta ctggagggct
     3961 tgcaaaaagt aaagaactag taaaaatgat agaagaaata ctgggtaaaa aaatttttat
     4021 tgcagaagat actcaaataa taggagcttt aggagctgct gtaataggat ttagataaaa
     4081 acatataaaa agaaaatgag aagctgaatt tattataaat aaattcagtt ttttatttta
     4141 ttttatttat ttataaagga aaactttggt ataattgatt taaatgattg tatatttgta
     4201 aatacttgat atttagtcat atataacatt ataattttaa aaattagctc aaaataaggg
     4261 ggagaaagcg tgaaaaaatt tacttcaaaa aaagtaacaa ggtttgttgc gctgttttta
     4321 atatctattt tagtaatgtc aacaattcct gtatcagctg acaatagcaa tacattgaat
     4381 cagataaaaa aagcagaata cagtgagacg tataaacaat atctaaatga tgctaaaaat
     4441 ggatgtacag aaaaatataa tgggattata ccaaatccat atcagttgga aggtactcaa
     4501 atccaaagta aaggaagaac agctccaaca tcttatgacc ctagaaaatt aggtcttatg
     4561 acatcaataa aaaatcaaga ggatttagga atctgttggg attttgctgc aatggcaacc
     4621 ttagaaagtt tcttaaagtt aaataattat ggagactatg atttatcaga agaacactta
     4681 agatggtggg cttcagatgg tgaatatgga tggtctgtaa atgatatgaa tggtgcttta
     4741 aattatgaag ctatgggata tttaacatct tggtcaggtc ctaaattaga aaaagatatc
     4801 ccatacaatg gtagagtatc taaagcacaa ggagcaaaaa aaccaactaa tatgaataca
     4861 gcacctactg tttttaatgt gacagatgct gtgtgtgtaa gcaatgacat aaattctaca
     4921 aaaaatgcta tattacagta tggagctgta acatctggtt attatgaaga tattaaatat
     4981 caaagtgatg accaaaattc atattattgt ccaacaaaaa gttttaatac aaatcatgct
     5041 atttcaattg taggttggga tgataactat tctaaagata attttaatgc aaatattaga
     5101 ccatcaaaaa atggtgcttg gttaataaaa aatagctggg gagattataa ttctgagggt
     5161 ggatatttct ggatatctta tgaagacaag acacttatgt ctgatataga taatttttca
     5221 atcaaaggtg gtaaaaaacc aaatgatgat gagaagatgt atcaacatga ctatgcaagt
     5281 attgtacctt taatttcaaa aaagataaca gctgcaaatg tatttgattt caatagaggc
     5341 gatgaaactc taaaatctgt aatgttctta actgaaagta taggagctaa gtatgaagta
     5401 tattatgctc cagtagtaaa tggtatacct caagaaaaca atatgaaaaa attaaaagaa
     5461 ggtactgccc aatattcagg atacataaca gttccaattg attcttttga tataccagaa
     5521 ggaaagggtg caattgtagt cagtatagag gggaaaaatg gagagtcaac tataggttct
     5581 gagtcaaatg taccaggata tgatacattt aaagcaaaag ctaatttagg tgaaagttat
     5641 atcatagata atactggaaa attctttgat ataaatagag atagtaactt ctatccgtgt
     5701 aattttacta taaaagctgt tacagaaaag tcttctggtg agagtatccc aaatgagtct
     5761 ttaattggta gtgatagata tgaaactgct atcaaagtta gtcaaaatgg tttcaattct
     5821 tcagaaaatg tagttttagt aaatggcagt tcaatagtag atgcattggc agcaactcct
     5881 tttacatctg ctataaattc accaatatta ttgacacaaa aagaagcttt aaattcaaaa
     5941 actaaagctg agatacagag attaggtgct aagaaagtat acctaatagg tggagaaaat
     6001 tctataagta aagaaattga acaacaatta aaaagcttaa atatatcgat agagagaata
     6061 tcaggaagtg atagatacaa gacaagttta ttattagctc aaaagttaaa tggtataaaa
     6121 aatgtatctc aaatagcagt tgtaaatgga gtgaaaggat tagctgatgc aattagtgta
     6181 ggcgctgcag cagcagaaaa taacatacca atcatacttg caaatgaaaa gagcgaattg
     6241 caaggagctg atgaatttct aaattcatta aatatagaga aatcttatat tattggagga
     6301 acagcatccc tatcaaacaa tctagaaagt aagttaaaaa atccaactag attatctggt
     6361 agtagtagag acgaaactaa ttcaaagata atagataatt tttataaaaa agatacttta
     6421 aaaaatgcat ttgtagttaa aaatggaatc aagaatcaaa atgatttaat agatggattg
     6481 gcagttggtc ctttaggagc aaaaactggg tcaccagtaa ttttagttgg tgataaatta
     6541 gctgatagcc aaaaagaagt tcttaaaaat aagactttag aaaaagtaac tcaagttggt
     6601 ggaggagcaa ataaaaatgc atttaaagag ttagtaaaat taaaaagtgc taaatagagt
     6661 acttaagtat taacatatat tagtgtgtat tagtaactta tcttaataaa agctgaaaat
     6721 aattacttca aattattgat aaataagata atcaatgatt gtaaagtttt gttttcagct
     6781 ttaaatttat gtagtataag tttaaaaaca taaaaaaggg ttactttata tagaaatata
     6841 gaaaatctta cattttatat ataaataacc catatttaat taacttttta tagtgttcta
     6901 atagatatta t
//
LOCUS       DAEPIN010000129         6863 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.131, whole genome shotgun sequence.
ACCESSION   DAEPIN010000129 DAEPIN010000000
VERSION     DAEPIN010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6863
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.131"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            243..1646
                     /locus_tag="KR250_003588"
     CDS             243..1646
                     /locus_tag="KR250_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBH0069468.1"
                     /translation="MEKKKIGLWSLVFMNVSALYGIRWIAKSTASSFGLGLGAIPMWL
                     LFSFIYFVPGALICAELASTYQKGDGGLYDWVKEAFGEKYGFLVSWLNWTAKIFWYSS
                     FLTFLAVNVSYTIGNPALADNKMFVLILSLIIFWALSLIATKGISFTKIFTNTGALGS
                     TIPSAILIIFSFVAVFVLKKHDIASTYTIASMTPKLNMDAFVAISAIMFGLAGAETAA
                     NFITEIDKPEKNFPKAILISAGIVASLYVLGSIAITMIIPPDQITASKGVLDALSAVC
                     ASLGIGSWLVQLIAFGIAFSVLGAIVLYIASPIKMLFGSVKKGIFPDSLVEVNEHKIP
                     SKAVILQAIIVTIILLVTTLMPSVDAIYNVLVTMTALTALFPYVLLYASYIKLRKERP
                     DEIRPYTMAKSTSTCLGLAKMVLVVTVVGILLSAAPVMPTFAENVIYEIEMIGGGLLV
                     ILSGLWLWNRYEKKTAK"
     misc_binding    1737..1977
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2020..2718
                     /locus_tag="KR250_003589"
     CDS             2020..2718
                     /locus_tag="KR250_003589"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HBH0069469.1"
                     /translation="MNVIGIIGAMDEEVSILVDLMDIRETIKKASLEFYKGILEGKNV
                     VLVKCGIGKVNSALCAQILISEFKVDAIVNTGVAGALNEKLDVNDIVISTDAIQYDVD
                     TTAFGDPKGVIPRMKTSVFKADERLIDAAYKSSVEEVKTHKVLKGRVVTGDKFINSKE
                     LKEELVNDFGGYCGEMEGGAIAHVCYLNNTPFVIIRAMSDKADGSADVTYDVFVHDAA
                     NNSKDIVLNMLKSI"
     gene            2741..3742
                     /gene="trpS"
                     /locus_tag="KR250_003590"
     CDS             2741..3742
                     /gene="trpS"
                     /locus_tag="KR250_003590"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HBH0069470.1"
                     /translation="MSEKKVILSGAQPSGKMTLGNYLGAIKNWTELQDNYDCYYSIVD
                     LHAITVPQDPKVLRANTIELLAQYIACGLDPEKNTIFIQSHVKEHVELMWVLNTMTYM
                     GELSRMTQFKDKSQKSEANLNAGLFTYPVLMAADILLYQTDLVPVGDDQKQHLELARD
                     LANRFNNRFSPTFVVPEGYYPKGGARVMSLQEPTKKMSKSDSNENAFILLADDSDSIK
                     RKIKRSVTDSLGVVKYNDEQPGIKNLIDIYSNLSKKSVEEIVNMYEGKGYGIFKEDVA
                     EVVSEALRPIREKYVDLLNNKDYLEKIYSMGAEKAEKQARKTLRKVYKKVGFIERRY"
     gene            complement(4394..5961)
                     /locus_tag="KR250_003591"
                     /pseudo
     CDS             complement(4394..5961)
                     /locus_tag="KR250_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021426623.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iota toxin protein Ib"
     gene            complement(5936..6079)
                     /locus_tag="KR250_003592"
     CDS             complement(5936..6079)
                     /locus_tag="KR250_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069471.1"
                     /translation="MYVYYFESPEKFAFNKVIRTKNQNEISLEKFNEFKETIQDKLLP
                     YNR"
     gene            complement(6297..6479)
                     /locus_tag="KR250_003593"
     CDS             complement(6297..6479)
                     /locus_tag="KR250_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069472.1"
                     /translation="MKKFRKHKSISNCISILLILYLTLGSLLPNNIYAQDLQSYSGKV
                     CNTTYKAPIERSEGFF"
BASE COUNT         2443 a          805 c         1044 g         2571 t
ORIGIN      
        1 tttataataa aataatattg aaacaatttt aaaaatttag tatcattaga attaaagtgg
       61 taaaactttt aaatttatca tttttaataa gatttgattt caaagtattt aaggtttatt
      121 attaaaactg tttttgaata tgcaaatatt ttgaaaaata tcagtttaat aataatgaat
      181 ctattttatt aattttgaaa ttaaatttaa aataattaat agagagggga aattaaatta
      241 ggatggaaaa gaaaaaaata ggcctatgga gtttggtatt tatgaatgta tcagctttat
      301 atggtataag atggatagct aaatctacag catcaagctt tggtttagga ttaggtgcaa
      361 tacctatgtg gttattattc tcatttatat actttgtacc aggagcttta atttgtgctg
      421 aattagcttc tacatatcaa aaaggtgatg gaggtcttta cgattgggtt aaggaagcct
      481 ttggagaaaa gtatggattt ttagtttctt ggttaaactg gacagctaaa atattttggt
      541 attcttcatt tttaactttc ttagcagtaa atgtttctta tacaatagga aatccagccc
      601 ttgctgataa taagatgttt gtacttatat tatctttaat aatattctgg gcattatctc
      661 ttatagcaac taaaggaata agttttacaa aaatatttac taatacaggt gcattaggtt
      721 caacaatacc aagtgctata cttataatat tttcatttgt agcagtattt gtattgaaaa
      781 aacatgatat agcttctaca tacacaatag cttcaatgac tccaaaactt aatatggatg
      841 catttgttgc tatatcagct ataatgtttg gtttagcagg agcagagact gctgctaact
      901 tcataacaga aatagataag cctgaaaaga acttccctaa ggcaatatta atatcagctg
      961 gaatagtagc ttcattatat gtattaggtt caattgcaat aacaatgata ataccaccag
     1021 accaaattac tgcttctaaa ggtgtattag atgcactttc tgctgtttgt gcgtctttag
     1081 gaattggttc ttggttggtt caattaatag cttttggtat agctttttca gtattaggag
     1141 caatagttct ttacatagca tcaccaataa aaatgttatt tggtagtgtt aaaaaaggaa
     1201 tatttccaga tagtttagta gaggtaaatg aacacaagat accttctaaa gcagttatac
     1261 ttcaagcaat aatagttact ataatactat tggtgactac attaatgcct tctgttgatg
     1321 ctatatacaa tgttcttgta actatgacag cattaactgc attattccca tatgtattgt
     1381 tatatgcttc ttatattaaa ctaagaaaag agagaccaga tgaaattaga ccttatacta
     1441 tggcaaaatc aacaagtact tgcctaggtt tagcaaaaat ggttttagtt gtaactgtag
     1501 tgggtatatt attatctgca gcaccagtta tgcctacatt tgctgaaaat gttatatatg
     1561 agatagagat gattggtgga ggtcttctag ttatattatc tggtctatgg ttatggaata
     1621 gatatgaaaa gaaaacagct aaataaaatg tattttaaaa atttcttttg gatattataa
     1681 tatatagttg acaatttata gtgtaaggaa tattataaaa taaagaaatt aataaattaa
     1741 atcctgagat aagaaatagt aaataaatat atttttgcac agagaattaa ggatgctgag
     1801 aacttaataa aaagtattta ttgaatgggt ctttgaggag taaaatgaaa tgtaatttaa
     1861 gattacagag tatttgatat cgtgtatgca cgttatagca tatattaaga tatatatatt
     1921 caattttgag tgtatataaa tttaggtggt accacggaaa atatatccgt cctattgatt
     1981 taggacggat tttttaatat agaaaattta ggaggaaaaa tgaacgtaat aggaattata
     2041 ggtgcaatgg atgaagaagt tagtatttta gttgatttaa tggatattag agagactata
     2101 aaaaaagcta gcttagaatt ttataaggga attttggaag gtaagaatgt agtgttagta
     2161 aagtgtggca taggtaaggt taactctgct ctttgtgccc agatactaat aagcgaattt
     2221 aaggttgatg ctattgttaa tacaggtgtt gcaggagctt taaacgaaaa acttgatgtt
     2281 aatgatatag taatatcaac tgatgctata cagtatgatg tagacactac agcatttgga
     2341 gaccctaagg gagttatacc aagaatgaag acttctgtat ttaaagcaga tgaaagactt
     2401 atagatgctg cttacaaatc ttctgtggaa gaagtaaaga ctcataaagt attaaaagga
     2461 agagtagtta caggagataa atttataaat tctaaagaat taaaagaaga attagtaaat
     2521 gattttggtg gatattgtgg agaaatggaa ggtggagcta tagctcatgt atgctattta
     2581 aataatactc catttgtaat aataagagca atgtctgata aggcagatgg aagtgctgat
     2641 gttacttatg atgtatttgt acatgatgct gccaataact ctaaagacat agttttaaat
     2701 atgttaaaat caatataaat atataaggag gtacaaaaaa atgagtgaaa aaaaagttat
     2761 attaagtggt gcacagccat caggcaaaat gactttagga aattatttag gagctataaa
     2821 aaactggact gaacttcaag acaattatga ttgttattat agtatagttg atttgcatgc
     2881 aataacagta cctcaagacc ctaaagtttt aagagccaat actatagaat tacttgctca
     2941 atatatagct tgtggattag acccagaaaa aaatactata tttatacaat cacatgtcaa
     3001 agagcatgtt gaacttatgt gggtattgaa tactatgact tatatgggtg aattaagtag
     3061 aatgactcaa ttcaaagata aatcacaaaa gagtgaagca aatctaaatg caggtttatt
     3121 cacatatcca gttcttatgg ctgctgacat attgttgtat caaactgacc tagttccagt
     3181 tggagatgac caaaaacaac atttagaatt agctcgtgat ttagcaaata ggtttaataa
     3241 tagattttca cctacttttg tagttccaga aggatactac ccaaaaggtg gagcaagagt
     3301 gatgagtttg caagagccaa ctaaaaaaat gagtaaatcc gattcaaatg aaaatgcatt
     3361 tatactatta gcagatgatt cagattctat aaagagaaag ataaaaagaa gtgttactga
     3421 ttctttaggt gttgtaaaat acaatgatga acaaccaggt attaaaaacc taattgatat
     3481 atattctaat ttaagtaaaa agagtgtaga agagattgta aacatgtatg aaggaaaggg
     3541 ttatggcata tttaaagaag atgtggctga agtagtatca gaagctttaa gacctataag
     3601 agaaaaatat gttgaccttc ttaataataa agattattta gaaaaaatat atagtatggg
     3661 agcagaaaaa gccgaaaaac aagctagaaa aactcttaga aaagtttata aaaaagttgg
     3721 atttatagag agaagatact aattattagc tatctttact tgataaataa attttaaagt
     3781 tattaaaaag gtatatgata tgaaagagag agtctcaaag tgaacttttg ttcatgaggc
     3841 actctttttt gtatgaaata aaatatattt taatcatttc aacaatgaaa aagaggatta
     3901 tctctatttt gagacagcat cttttgataa taaaaaaaga tgagcttttg ctcatctcaa
     3961 aggggttaga ttattcttac tttggggaaa gtagaataaa ggtaaaagtt aactgatgtc
     4021 aactttgtct tttttgcata tcactactga gcataaaaaa atcagaaaac catagccttc
     4081 tgaaacaact tctaacatac attgtatttt atacccaata tgtaatttat gagttaattt
     4141 taactcttat aatgaaaatt gtcaataata tatcaaaaaa atatttttta aatttatata
     4201 attaaatgaa tttatgttta tatataggtg atatgataca gataattatc tttttaatac
     4261 aatatagttc taaaaaagaa tatattagaa gaagataata tttaaataaa aaatacctta
     4321 taaagttaat tttttaatac tttataaggt aaatacatat tgtattgtgg ggataaattt
     4381 aaatcatttg atactaatca acactaagaa ccaataactc tctatcatct tgagtaattg
     4441 tatatattct taatttcttg tatgtcataa tattttctcc acctgtaaaa taactcctaa
     4501 gattaacaca atcagtttta ggttgatttg tatctcctaa ataattacgc aattctttat
     4561 ctttagtttc aatatcttta aatccaaaat attgtaaagt agcttcaact ctatattttt
     4621 ggacataatc gagagcttct ttattagttt atgttggtgt aaaatacata ccatttatat
     4681 aagcctttat tgtacttgga ttaaatttaa aatctgcaga atatatttta tgtgtaacta
     4741 ttatttcttg aacagttgga attttaactt ctggttcatc aagtatttca ggagtactat
     4801 ttagctctgt aatagataag ttatctaagt atattgtatc actatcaatt aatgctatat
     4861 ttatattaga agaatctttt tcagtagttt caaattcata actaaatttt gtatatcctt
     4921 gttctagtac caaataatcc gttttttctt cttttacttt tatatttaca attattgaat
     4981 tatatgttaa aggatttttt gaattttact aaaaacataa cgtttataag gttttagttc
     5041 agacataggt attttaatct tcgtctcacc atttaatcta tttgctgagc cttttaaacc
     5101 atcttgattt gtagtattaa cattactcca tatactaggg taattattgt aatcatcaaa
     5161 attagtaaag taagttggtg tttttataag tatattcatt cctctttcaa gtttaacatt
     5221 acatatttgt ttatcagaca aagtttttaa actgtcttta atcttattgc ctatattatc
     5281 atcaaatatg agttcaacac aactttcatc tattggtata ttattaaaat ataacaaacc
     5341 atctttttta gtagtgccaa aagttttttc aattgcttct ccaattgtaa gttcaggtgt
     5401 tttatcttct gggacctgta aacttttagc agtaattctt cttttgtaag attcattttc
     5461 tgtatctaat ataatagata cagaaatact gtcaatttga cctatatcgt cttaccaact
     5521 atttcattct gttactattt gtccagaacc attttttgta ccaaaatttc cacttacttg
     5581 tgttgtttct aatttaattt gctttccatc atctaatttt tttaattaat tataatttat
     5641 aggaatcagt ctagaactaa attgatacat tgtgttaaga gccataggtg aaaatccttt
     5701 tttgagataa gtatttccag gagatagatt attgccaatt tgattttctt gtgctttgat
     5761 agttgataat gcatctccat ctaacactaa atttgttgtt ggtgtcactt tgtacatagg
     5821 tgcagtacct gtgttgtagt atctaatatt tgcatttata tatgcagatt ctcctttgtt
     5881 tatacttaat ccagtatttc atgattatcc attgctattt tgaacagcag ttgaattatc
     5941 tgttgtatgg taataatttg tcttgtatag tttctttaaa ctcattaaat ttttctaatg
     6001 aaatttcgtt ttgatttttt gttcttatta ctttattaaa tgcaaatttt tctggagatt
     6061 caaaataata tacatacata ggtttatcta ttttattttt tgatatagca tttcgaagtt
     6121 ctctatattc tttttctcaa gaatttgctt ctatttgata atcataaaaa taatttcttg
     6181 tctgagaata tttgcttatc tctatagaat cttttttata tgatcctaat acttcttttt
     6241 cagatttttc aagtttttga tcttttcttt ctcattcttt agccttttct ttatctttaa
     6301 aaaaatcctt ctgatctttc tataggagcc ttgtaagtag tattacaaac ttttccacta
     6361 tagctttgta aatcttgtgc ataaatgtta ttaggtaaca aactacctaa agttagatat
     6421 aatatcaaca atatagatat acaattacta atacttttat gtttcctaaa ttttttcatt
     6481 tattctccct acaaatatta gtttagttaa cttttgaatt ttaatttatt tagattatat
     6541 aatctaaata agaatttttt atataaattt cataaaatat gtaggtatat atcataaaaa
     6601 tatgtattta tatgatatgt cagtacaaaa taacaactga atattttaaa gagactattt
     6661 cgtactttta tgatttaatg agctatctta tcgaatcata atctaggaac aattgttata
     6721 atatctaata gtagttatat atgatatagt cagtatgtgt tttttcacaa tatgattatt
     6781 atatagagga ttcttaggga ttactctttt tgtataaaaa agagatggtt caaattttta
     6841 tcttgagaca tctccttttt cta
//
LOCUS       DAEPIN010000130         6826 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.132, whole genome shotgun sequence.
ACCESSION   DAEPIN010000130 DAEPIN010000000
VERSION     DAEPIN010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6826
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.132"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            complement(<1..161)
                     /locus_tag="KR250_003594"
     CDS             complement(<1..161)
                     /locus_tag="KR250_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH0069473.1"
                     /translation="MIAVKKAYKFRMYPNKKQQELINKTFGCCRFVYNKYLAKRIDVY
                     KNDKETFTY"
     gene            complement(809..1216)
                     /locus_tag="KR250_003595"
     CDS             complement(809..1216)
                     /locus_tag="KR250_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069474.1"
                     /translation="MSKVAKATFYLMIVTIISKILGMGRELVLSSIYGTGLYTESYLT
                     AMNIPNIIFAAIGTAIVTTFIPMYQDISSKQGEKQALKFLNNVLNIIVGICIVVAILG
                     VIFSKQLVSIFAIGFEGERFLLTVKFTKILIKK"
     gene            complement(1690..2574)
                     /locus_tag="KR250_003596"
     CDS             complement(1690..2574)
                     /locus_tag="KR250_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBH0069475.1"
                     /translation="MDTKKENNYENYICVGSSNVSNAMKMYLKEIEEYKMLSAGEEVE
                     LAKEIINSSSVAKEKFINSNYRLVVSIAKRYKRDSIDMLDLIQAGNIGLIKAVEKYDY
                     KKGYKFSTYATWWIKQSITRYIDDCENTIRIPIHLHQRINFVKKKKQELLNVLLREPT
                     IDEIADACGLEVDKVLELLRRDKNVVSLDTPLKEDEDSSLVEFIPSDADFKDVVIHEV
                     EQHNLKEKIEELLTGLGEQEQQVLRMRFGIDDDDPKTLEQIGKVFGVTRERIRQIEAK
                     AIRKLRHPSKLKQLKHFY"
     gene            complement(2865..3341)
                     /locus_tag="KR250_003597"
     CDS             complement(2865..3341)
                     /locus_tag="KR250_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HBH0069476.1"
                     /translation="MSKELVKDRVIKYLIEDLLVPQDMIDTNVELAEFEEGAEGILDI
                     VVNVKDEEDYYAPVMIVQCLDEDVELEGEVLQKQIEFLEEVDNITMSGRLVLTNGDAM
                     MYADWRGEEYDTEAALPTYDIMVKEFHEMEQQVKDLEEHHHHDENCGCGCNHHHEN"
     gene            3607..4782
                     /locus_tag="KR250_003598"
     CDS             3607..4782
                     /locus_tag="KR250_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HBH0069477.1"
                     /translation="MKFKKQISSIVVSTMLVLGSMNLSYADGTNVVTLGANLSESQKQ
                     QMLNYFGVKKDQVLVLDVTNAEERQYLQGVATEGQLGKVTISCSYVEPTKKGNGINVK
                     TANLTWVTSSMIASTLTTAGLEDANVIAAAPYPVSGTGALTGIMKAFEDASGKKLDET
                     KKEIASEELVVTGDLGDDIGQEKATGVMNDIKTEIVKNGTKDTNQIANTINNVVNNYN
                     ITITPEQKEQIKGVMKKIADQNYDYNSMKNTLDNVSDNVNEQLKKLGESVKGSGILDT
                     IGGWFSGIGDWFSGLFSGGDKKDLGILNNTNDESLGSDAVINSTEGVQINPQNNTNSN
                     NDSDTNSSKNDTSSNNENNDSSQTNENNTTNNSENQSNNNDSVGFFEKIKNWLSGLF"
     gene            complement(4863..5666)
                     /gene="proC"
                     /locus_tag="KR250_003599"
     CDS             complement(4863..5666)
                     /gene="proC"
                     /locus_tag="KR250_003599"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HBH0069478.1"
                     /translation="MKKIGFIGAGNMASAMIGGIVNSKLVEPNMVIASAYSQGTLDRI
                     DANFGINTTKDSKEVTRTSDIVIVAVKPDIYDDILEEIKDFIDDNKIIVTIAAGKSIK
                     DIESIIGEDKKIVRTMPNTPALVNEAMSSLSINKNINKEDLEAVTEVFNSFGNTEVVP
                     EYLIEAVIGASGSAPAYVFLFIEAIADAAVIAGMPRPQAYKFASQAVMGSAKMVLETG
                     KHPGELKDMVCSPGGTTIEAVKVLEEEGFRASVIKAICACIEKSKKMSE"
     gene            complement(5706..6290)
                     /locus_tag="KR250_003600"
     CDS             complement(5706..6290)
                     /locus_tag="KR250_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0069479.1"
                     /translation="MNKDIGAKIKQLRTQKQMTLKDMSEKTNLSIGFLSQLERGLTSV
                     ATDSLGKIASVLDVELTYFFMKPKEHKRAVLRSYEKEVFDVENSTFIHYHLSSSLKEK
                     TMLPRLIEILPSKSSEEICCYVHEGEEFVYVLEGTLTVFLGDEQIEMYPGDTIHYNSE
                     KNNHNWVNYTNKVVKILVVSIPNPFEKSDAVKEA"
BASE COUNT         2248 a         1147 c          759 g         2672 t
ORIGIN      
        1 ttataagtaa aagtttcttt atcgttttta taaacatcta ttcttttagc aagatactta
       61 ttatatacaa atctacaaca tccaaaagtt ttgttaatta actcttgttg ttttttattt
      121 ggatacattc taaacttata agcttttttc actgctatca cctcacttta attgtttttt
      181 gatacacttt ttaatgattt tatcacattt tatcacattt aatatattat ataatgctgt
      241 ggtaaacaat ttattaatca cctataataa tgttaacaaa tgtaattatt taaaacaatt
      301 atttagaagg ttttatattt aattttaaat aacatattta agaaccgtaa gtcttaactt
      361 ttatcatttt gaggttgtcg ttcacataag ttcgctacta cctatgcagt tctcttatga
      421 acttcttaca ttttcatgta agcacagact atatcttatc cttcagcatt atccgttaag
      481 gtctacccac ttccactacc aatagcttgt agtgtacttc cctcaagagg aatagtcgtt
      541 gaagtttctc ctgttcggag tttacctgct gattgcccat tttaaagtac ttaggattta
      601 accttatacc atctaaccaa ttttttctac tttcgtaact ttcacactta ggtttatttc
      661 atccttatgt tttagtttga ttagctttag gggtttccag caattcaagt agtattggat
      721 aggttctatc ctatctctac atataggttt ccctatatgc tgactatttt agctactaac
      781 tcatgactaa agtcacgagt gtgtgtagct attttttaat caatatttta gtaaatttta
      841 cggtaagtaa aaacctctct ccttcaaatc ctatagcaaa tatacttaca agttgctttg
      901 agaaaataac tcctaatata gctacaacta tgcatatacc aactattata tttaaaacat
      961 tgtttaaaaa ctttagtgct tgcttttcac cttgtttact acttatatct tgatacattg
     1021 gaataaaagt agtaactata gcagtaccta ttgctgcaaa tataatattt ggtatattca
     1081 tagctgttaa ataactctct gtatataagc ctgtaccata aattgatgat aatactaatt
     1141 ctctacccat tccaagaatc ttagagatta tagtaactat catcaaataa aaagttgctt
     1201 tagctacctt gctcatttta tcactccact tctaacatac ctctttttaa tcaagtaaaa
     1261 tttagctcat tagatataat tctatcaact aatttgcctt gcttagccct atcaaaatat
     1321 aattaacacc tatttcttaa tatatcacaa actacattat tcttcatatt tttttatttc
     1381 tccaataagt tttacatttt tcctatataa attgtatagc ttcaattata ttcaaagaaa
     1441 tggagttgct acccgataac tttaaaagta aaaaacagat tttattatct ttttaaacta
     1501 tattctaaca tattttgtcc atattgagaa attttactaa actattatgt actttattaa
     1561 ataaataaaa taacagcaca cctataaaat acaagtgtgc tgttcataaa cagattttat
     1621 aaaaaaataa ttcctaagta gagattaaca tgctttctca tactctcata tataatgtgt
     1681 ctaaaaatat taataaaaat gttttaattg ttttaactta cttggatgtc ttaattttct
     1741 tattgcttta gcttctatct gtcttattcg ttctctggta actccaaata cctttccaat
     1801 ctgttctagt gttttagggt catcatcatc aattccaaat ctcattctta aaacttgctg
     1861 ttcttgctct ccaagacctg taagtagttc ttctatcttt tcttttaaat tatgctgttc
     1921 aacttcgtgt attacaacat ctttaaaatc agcatctgat ggtataaatt caactaaact
     1981 actatcctca tcttctttta atggagtatc caaagataca acatttttat ctcttcttaa
     2041 taattccaac actttgtcaa cttctaagcc acatgcatct gcaatctcat ctatagttgg
     2101 ctctcttaat aatacattta aaagttcttg tttctttttc tttacaaaat ttattctctg
     2161 atgaagatgt ataggtatcc taatagtatt ttcgcaatcg tcgatatatc ttgttatact
     2221 ttgttttatc caccaagttg cataagtgct aaatttgtat ccttttttat aatcgtactt
     2281 ttctacagct tttataagtc ctatatttcc tgcttgaatt aaatccaaca tatcaatact
     2341 atctctttta tatctctttg ctatgctcac taccaaccta taatttgaat ttataaattt
     2401 ttcttttgct actgaagatg agttgattat ttccttagcc agctctacct cttctcctgc
     2461 tgacaacatt ttgtattcct ctatttcctt taaatacatt ttcatcgcat tcgatacatt
     2521 tgatgaaccc acacaaatat aattttcata attattttct tttttagtat ccataatcaa
     2581 accccttcta ttcatagaat ttgctaaaac aaaaataaat aatttaaacc tacataatct
     2641 acaattacat aatatcccat tctttaaatg aattgttttg tatattatgt atacaaattt
     2701 ataaaaacta tacctataat attattctac ttctaacagc aatatcctcc ttatatatat
     2761 aatttatttt attatatgtt gttatattct tagttttttg aatgatatat gtctaaaaaa
     2821 agactgttta ctaaacagtc tttttttata tatatgctca atatttaatt ttcatgatga
     2881 tgattacatc cacatccaca attttcatca tggtgatgat gttcttctaa atcttttacc
     2941 tgttgttcca tttcatgaaa ctcttttacc attatatcat aagttggaag agctgcctca
     3001 gtatcgtatt cttcccctct ccaatcagcg tacatcatag catctccatt agttaaaacc
     3061 aatctacctg acatagttat attatccact tcttctaaaa attctatttg tttttgtaaa
     3121 acttctcctt ctaactccac atcttcatct aaacattgca ctatcattac aggtgcataa
     3181 taatcttctt catctttaac atttacaaca atatctaaaa ttccttctgc accttcttca
     3241 aattctgcca actcaacatt tgtatcaatc atatcttgag gtactaataa atcttctatt
     3301 aaatatttta ttactctatc ttttactaat tcttttgaca tttatttttc tccttccgat
     3361 tctataacat tatcataatc atttctatat tatagtatac tacattaatt gttttttcaa
     3421 tctcttacaa aaattatttt ttattatctt ccttatattc ccttttttat tacaaagttg
     3481 taatcgcatt gatttaaaaa atagtttata tttagtataa tagaaatgta gcacatattt
     3541 ttattcaata tattaataaa aaaatatatt gaaaatgtaa tgtatgttaa atctaggagg
     3601 aatattatga agtttaaaaa acaaattagt tctattgttg tgtctactat gcttgtactt
     3661 gggagtatga acctttctta tgcagacggt acaaatgtag ttacacttgg agctaactta
     3721 tcagagtctc aaaagcagca aatgcttaat tactttggag taaaaaaaga ccaagtctta
     3781 gttttagatg taacaaatgc agaagaaaga cagtatttac aaggtgttgc aacagagggt
     3841 caacttggaa aagttactat ctcttgctca tacgttgaac ctactaaaaa aggaaatgga
     3901 attaatgtta aaacagcaaa cttaacttgg gtaactagtt caatgattgc ttcaaccctt
     3961 acaacagctg gtcttgaaga tgcaaatgtt attgctgctg ctccatatcc tgtaagtggt
     4021 actggagcat tgacaggaat catgaaagca tttgaggatg cttcaggaaa gaaacttgat
     4081 gaaactaaga aagaaattgc ttctgaagaa ttggtagtta caggtgactt aggagatgat
     4141 atagggcaag aaaaagcaac aggtgtaatg aatgatataa aaacagaaat tgtaaaaaat
     4201 ggtacaaaag ataccaatca aatagcaaat acaataaata atgttgtaaa taattacaat
     4261 attactatta caccagaaca aaaagaacaa attaaaggtg taatgaaaaa aatagctgac
     4321 caaaattacg attacaatag catgaaaaat acactagata atgtatcaga taatgtaaat
     4381 gaacaattaa agaaattagg tgaaagtgtt aagggttctg gtatactaga tactattggt
     4441 ggatggttta gtggaatagg agactggttc tctggtttat tctcaggcgg agataaaaaa
     4501 gatttgggaa tcttaaataa tactaatgat gagtctcttg gctctgatgc tgtaataaat
     4561 tctacagaag gtgttcaaat aaatccacaa aataatacta attctaacaa tgatagtgat
     4621 acaaatagct caaaaaatga tacttcaagt aataatgaaa ataacgatag ttctcaaaca
     4681 aatgaaaaca acacaacaaa taattcagaa aatcaatcaa ataataatga ctcagttggt
     4741 ttctttgaaa aaataaaaaa ttggctttca ggcttatttt agaaattaaa caaataaaaa
     4801 gaattgccta aaatggaaat tttctataaa aggcaattct ctttatttac aataaatttt
     4861 tattactcac tcattttttt agatttttct atacatgcac atattgcttt tatcacagaa
     4921 gctctaaaac cttcttcttc aagtacttta actgcttcta tagtagttcc accaggtgaa
     4981 cataccatat cttttaattc accaggatgt tttcctgtct caagtaccat ttttgcagaa
     5041 cccataacag cctgtgatgc aaacttatat gcttgtggtc ttggcattcc tgcaattact
     5101 gctgcatctg ctatagcttc tataaataaa aatacatatg ctggtgcaga accacttgca
     5161 ccaattacag cttctataag atactctggt actacctcag tatttccaaa agaattaaat
     5221 acttctgtaa ctgcttctaa gtcttctttg tttatatttt tatttataga aagagaagac
     5281 atggcttcat ttacaagagc tggagtattt ggcatagttc taactatttt tttatcttct
     5341 cctataatag actctatatc ttttatagat tttcctgctg ctattgtaac tataatttta
     5401 ttgtcatcta taaagtcttt tatctcttcc aaaatatcat catatatatc tggtttaaca
     5461 gctacaatta ctatatcaga ggttctagtt acttccttcg agtcttttgt agtatttatt
     5521 ccaaaatttg catctattct atcaagagtt ccttgactat atgctgatgc tataaccata
     5581 ttaggttcaa ctaattttga atttactatt ccccctatca tagcgcttgc catatttcct
     5641 gcacctatga atccaatttt tttcacttta gtacctcctt attgactaat agtaatcgtt
     5701 aaaatttaag cttctttaac tgcatcagac ttttcaaatg gatttggtat acttactacc
     5761 aaaattttca ctaccttatt agtatagttg acccaattat gattgttctt ttcactatta
     5821 tagtgtattg tgtctcctgg atacatttct atttgttcat ctcccaaaaa cactgtaaga
     5881 gtaccctcta atacatacac aaattcctca ccttcatgta cgtaacaaca aatttcctca
     5941 ctactcttac ttggaagaat ttcaattaat cttggtagca ttgttttttc ctttaaacta
     6001 gatgataaat gataatgtat aaatgttgag ttttctacat caaatacttc tttttcataa
     6061 cttcttaata cagctctttt atgctctttt ggcttcataa aaaagtaagt taattctaca
     6121 tctagtacac ttgctatttt tcctaaggaa tctgttgcca cacttgttaa tcctctttca
     6181 agttgtgata aaaaaccaat tgacaagttt gttttttcac tcatatcctt cagcgtcatt
     6241 tgcttttgag ttctgagttg ctttattttt gctcctatgt ccttattcaa aaccatcacc
     6301 cctaaataat tttaattttt tcataatata aatacaaaaa tataaatata ggtactgaac
     6361 ctatctttat ttcatttaaa aaaattttta taagtattac tataatttat caatcttttt
     6421 ttgtctacat ttttacgatt atttacgatt tatttttcac atatgtatat ttaatcttgc
     6481 caactaaaga tacatttttt ataagcattt actctttata taataaaact taagttgttc
     6541 atgagtcttc ttctggttta attattaaaa tttcaaatta tattattatt tctacacatt
     6601 attacatcct ttagtacaat tataacataa ttttacaatt taatgatatc tattcaatgt
     6661 ctttttttac tctacaacat attttatttt agagtttaat actatatata ttttttatat
     6721 acagccttgt cattttttag attgtcaaga gttattgaag tattttatgt agatttagat
     6781 tttattttct aatttctcat ttaacaagaa ttgcttatga taaaaa
//
LOCUS       DAEPIN010000131         6780 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.133, whole genome shotgun sequence.
ACCESSION   DAEPIN010000131 DAEPIN010000000
VERSION     DAEPIN010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6780
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.133"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            62..838
                     /locus_tag="KR250_003601"
     CDS             62..838
                     /locus_tag="KR250_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HBH0069480.1"
                     /translation="MEYIIFDLEFNQGFDKKLNKTVSNEKCPFEIIQIGAIKLDSKFN
                     IIDTFNSYVKPTIYKDIHPFISRMTNIKNSDFNDSPTFPEVYNNFIKFISSQDPILCV
                     WGAGDLKELYRNINYHKLPSNSLPKSYINIQQHASKYFNNPAGKSIGLQNAISILELD
                     EKMSYHNALNDAYYTAKVFIKIYNPSIVPDIYLYTSIKPKTIRYSNKKRVDYDKLFDE
                     FRKILNRELTKDEKKIINLAYNMGKTNQFTLENVKQRKNK"
     gene            complement(930..1682)
                     /gene="xth"
                     /locus_tag="KR250_003602"
     CDS             complement(930..1682)
                     /gene="xth"
                     /locus_tag="KR250_003602"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HBH0069481.1"
                     /translation="MKFISWNVNGIRACVGKGFLDFFKEVDADIFCLQETKLQEGQIE
                     LDLPGYFQYWNYAERKGYSGTAIFTKKEPLKVMYGINIEEHDKEGRVITLEFEDFYFV
                     TVYTPNSQSELKRLEYRTRWEDDFIDYLTKLDNHKPVIVCGDMNVAHKEIDLKNPKNN
                     MKNAGFTKEEREKFSKLLDSGFIDTYRYFNPDKEGVYSWWSYRFNARKNNAGWRIDYF
                     CASKKLEDRLVSADIHTEILGSDHCPVELEIK"
     gene            complement(1843..2793)
                     /gene="galT"
                     /locus_tag="KR250_003603"
     CDS             complement(1843..2793)
                     /gene="galT"
                     /locus_tag="KR250_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HBH0069482.1"
                     /translation="MKELRIDPITNDVVIFATDRLKRPLDTADIPNEDEETNEYDEEC
                     PFCKGNEAHATDALFEIESEEGWLVKSVYNKFPIIDDMARDVYGVHEVMIESDKHNRS
                     FYNMSQKEFEDVFFMYRNRFRDLSKDDKVEYVSIFKNFLRKAGASLMHPHAQILSMSF
                     IPPEITNELLVSKEYYDSNKSSLYDDLIENEISLDRRVVYNGEAFLVLIPYATKYSGE
                     VRIIFKDKIEFGELNDNNIQELSTIFEKLFKKLYNINGYMPFNLCIHTHPTKIETKSY
                     FNVHMHIIPRKYNFGGFELGTNMYVSSMSPEDLTKKLKID"
     gene            3178..4374
                     /locus_tag="KR250_003604"
     CDS             3178..4374
                     /locus_tag="KR250_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBH0069483.1"
                     /translation="MNYSNRVSAMQASPIRKLVPFAQAAKDKGIKVYHLNIGQPDIKT
                     PKGFFDAVKNFDSEVLEYATSEGIPELLEALQNYYKTYDMNFEKDELLVTNGGSEALL
                     FTMMAVCDPGDNLLVPEPFYTNYNGFGQSVNVEVNAVTTKAENGFHLPSKEEILSKVD
                     DKTKAIILSNPGNPTGAIYTKEELNILAEIAKEKDLWIIADEVYREFVYDGLEYTSCG
                     NLEGVQDRVIIIDSVSKRYSACGARIGSIACKNKGLIAQILKLCQGRLCVPTLEQIGA
                     VELYKTPVSYFKEVNEEYKKRRDVLYNELMKVEGVICKKPTGAFYIVAKLPVENAEDF
                     TIWMLKEFNKDNETVMVCPAEGFYATPGLGRDEIRLAYILNEKDLHRAATLLKEGLEQ
                     YVALQK"
     gene            complement(4486..5826)
                     /locus_tag="KR250_003605"
     CDS             complement(4486..5826)
                     /locus_tag="KR250_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HBH0069484.1"
                     /translation="MKKRYNIAIVGGGSTWTPGLLKSLCKMSDRFPLNKVTMLDVVEE
                     RQRLIGEFGKILFKEEYPKATLEYTTNPDEAFIDVDFVFVQMRTGGLKMRELDEKIPL
                     QFGLVGQETCGAGGFAYGLRSIGDMIEMVKTVRKYSPKAWILNYTNPAAIVAEALKRV
                     FPDDKRLLNMCDQPVNLLRSYGRLLDMDSRTFEPVYFGLNHFGWFTHLYDKNGEDLVP
                     KIKDLVANSGFQPVDAEQRDKSWLDTYGMVKDMLEDVPDYLPNTYVQYYLYPDKKVAK
                     SNINCTRAREVMNGREKRVFEECKSVIEKGTSIGSNLVHNDAHGEFIVEVAEAIAHNK
                     HQIAIVITENNGAISNLPDDAMVEVAAILTKNGPRPLHVGNIPTFYKGLLEGQLAYEK
                     LAVDAYFEQSYEKAILSLTLNRTIISPTKARQVLDALIKVNKDYWPTLYKENEK"
     gene            complement(5965..6717)
                     /locus_tag="KR250_003606"
     CDS             complement(5965..6717)
                     /locus_tag="KR250_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBH0069485.1"
                     /translation="MNLIDKLDINQKKLSTNEISLLNYFLKNSEKINQKKIKDIANET
                     FNSTAYIVRFCQKLGFSGYSEFKNFLVFSYSNRNERNKEFTKSQSDIFTDVMDTQNLI
                     NVENIDMCLNEIHNSKHIYFFGVGSSRLVCTEMSQRFSAIGVNVKYYDDSTLMYLAAS
                     NITKDDLVIAVSMSGETAQVIKACNIAKTNQVKVISLTNISVNSLSNIADIKLFVSSP
                     QYNFNSVNYTSRVPALSLMEYMFHKYIEKYNR"
BASE COUNT         2368 a         1072 c          821 g         2519 t
ORIGIN      
        1 ttttatttat aatataatct atagtaaaag aaataataaa aaaatttagg agataaactt
       61 tatggaatac ataattttcg atttggaatt taaccaagga tttgataaaa aactcaataa
      121 aactgtatcc aatgaaaagt gtccttttga aattatacaa ataggagcaa ttaaattgga
      181 ttctaaattc aatataatag atacgtttaa tagttatgta aaacctacaa tttataaaga
      241 catacatccc tttattagtc gtatgactaa tattaaaaat tctgatttta atgattcgcc
      301 tactttccct gaggtctaca acaattttat aaaatttata tcatcacaag accctatatt
      361 atgtgtttgg ggagctggag atttaaagga attatacagg aatatcaact atcacaagct
      421 accatcaaat tccttaccta aatcttatat taatatacaa caacatgcat ctaaatattt
      481 caataatcca gcaggaaaaa gcattggcct acaaaatgct atttctatat tagaacttga
      541 tgaaaaaatg tcttatcaca atgctttaaa tgatgcatat tatactgcta aagtttttat
      601 taagatatac aatccaagta tagtcccaga tatatatctt tatacaagta taaaacctaa
      661 aactataaga tattctaaca aaaaaagagt tgattatgat aagttatttg atgaatttag
      721 gaaaatttta aatcgtgaat taactaaaga cgagaaaaaa atcattaact tagcttataa
      781 tatggggaaa acaaatcaat ttacattaga aaatgttaag caaagaaaaa acaaatagag
      841 tttatttaga taaaaaatag gcatgtagat taaaaaacta gttaacttta aatttatatc
      901 tacagcccaa tttttatcat aattaatttt tattttatct ctaattcaac tggacaatgg
      961 tctgaaccta atatttcagt gtgtatatcc gcacttacta atctatcttc taattttttg
     1021 gatgcacaaa aataatctat tctccaccct gcattatttt ttcttgcatt aaatctatat
     1081 gaccaccaac tataaacacc ttctttatca ggattaaaat atctatatgt gtctataaac
     1141 ccactgtcta ataatttaga aaacttctct ctttcttctt tagtaaaacc tgcatttttc
     1201 atattatttt taggattttt taaatctatt tctttatgtg caacgttcat atctccacac
     1261 acaattacag gcttatgatt atctagtttg gttaaataat ctataaagtc gtcttcccat
     1321 ctagttctat attctagtct ctttaattca gattgagaat ttggcgtata tactgtaaca
     1381 aagtaaaaat cttcaaactc tagagttatc actctaccct ctttgtcatg ttcttctata
     1441 tttattccat acattacttt taaaggctct ttttttgtaa atatagcagt acctgaataa
     1501 ccctttctct cagcataatt ccaatattga aaatatcctg gtaaatctaa ctctatttgc
     1561 ccttcttgca atttagtttc ttgtaaacaa aatatatctg catctacttc tttgaaaaag
     1621 tctaaaaatc ctttcccaac acaagctctt attccattaa cattccatga tataaatttc
     1681 atacttatac tcctcatcta aacattgaca cactccccat agctaaagat gggggctttt
     1741 caacaatttt attgtaaatt cttaattcac ttttatttta acaatgctat actataattt
     1801 catctaaaat tactatttac ttaataccta aaaatatatg ttttaatcaa tttttaactt
     1861 ttttgttaaa tcttcagggc tcattgaaga aacatacata tttgttccaa gttcaaatcc
     1921 tccaaaatta tattttcttg ggattatgtg catatgaaca ttaaaatacg attttgtttc
     1981 aatctttgtt ggatgagtgt gaatacataa attaaatggc atatatccat tgatattata
     2041 taacttctta aacaactttt caaatattgt agaaagctcc tgtatattat tatcatttaa
     2101 ctcaccaaac tctattttat ctttaaatat tattcttact tctccactat acttggttgc
     2161 atatggtata agtaccaaaa aagcttctcc attataaaca actcttctat ctaaacttat
     2221 ttcattttct attaaatcat catacaaaga gcttttattg ctgtcataat actcctttga
     2281 aaccaacagt tcattcgtaa tttcaggtgg aataaaagac atagacaata tttgagcatg
     2341 tggatgcatc aaagatgctc ctgcctttct caagaagttt ttgaagatac taacatactc
     2401 tactttatca tctttcgaca aatctctaaa tctatttcta tacataaaaa aaacatcttc
     2461 aaattctttt tgactcatgt tataaaaact acgattatgt ttatcgctct ctatcattac
     2521 ttcatgaaca ccatatacat ctcttgccat atcatcaatt ataggaaatt tattgtagac
     2581 agattttaca agccatcctt cttcactttc tatttcaaat aatgcatcag ttgcatgagc
     2641 ttcatttcct ttgcaaaatg ggcattcttc atcatattca ttagtttctt catcttcatt
     2701 tgggatatct gctgtatcta aaggtctttt taacctatct gtagcaaata taacaacatc
     2761 attggtaatt ggatctattc ttaattcttt cataattaac acctcttagt taatagcctt
     2821 taatataatt atatgatatt tatcgtatct gttaaataaa tttataatat tttttataat
     2881 tttgcatatt tattattata tattttgtct atagctgaaa gattataact aaaaaatcac
     2941 tatcagtata tcagaatatt atcttactat aaattaattt atgaatacat aatttagtgg
     3001 cataaacatg ggtattttac tataatttgt taaaagttta ttttaaagct atattaattt
     3061 tttttgatga tttttaccca taagtatgtt attattatct tgtaaagatg tcttgtattc
     3121 aaacatacaa tttacataat acaatatacg tatttaaatt aaataggggg tattaaaatg
     3181 aattattcaa acagagttag tgcaatgcaa gcctctccaa tcagaaaact tgttccattt
     3241 gcacaagcag caaaagataa aggaatcaaa gtatatcact taaacatagg acaaccagac
     3301 ataaaaactc ctaaaggatt ttttgatgct gttaaaaact ttgatagcga agtcttagaa
     3361 tatgctacat cagaaggtat tccagaatta ttagaagcac ttcaaaatta ctataaaact
     3421 tacgatatga attttgaaaa agatgaatta ttagtaacta atggtggtag tgaagcttta
     3481 cttttcacta tgatggcagt ttgtgaccca ggagataatc tattagttcc agaaccattc
     3541 tatacaaact acaatggatt tggtcaatct gtaaacgtag aagttaatgc tgttacaaca
     3601 aaggcagaaa acggtttcca ccttccatct aaagaagaaa tattgtctaa agtagatgat
     3661 aaaactaagg ctataattct ttctaaccca ggaaatccaa ctggtgcaat ttatacaaaa
     3721 gaagaactta atatcctagc tgaaatagct aaagaaaaag atttatggat aatagcagat
     3781 gaagtttata gagaatttgt ttatgatggg cttgagtata caagctgtgg taacttagag
     3841 ggtgtacagg atagagttat aataatagac agtgtttcta agagatacag tgcttgtggt
     3901 gctagaattg gttctatcgc ttgcaaaaat aaaggtctta tcgctcaaat ccttaaatta
     3961 tgtcaaggaa gactttgtgt tcctacatta gagcaaatag gagctgttga gttatacaaa
     4021 actcctgttt cttacttcaa agaagttaat gaagagtaca aaaaaagaag agatgtatta
     4081 tacaatgaat taatgaaggt tgaaggtgtt atatgtaaaa aaccaactgg tgctttctat
     4141 atagttgcta aattaccagt tgaaaatgca gaagatttca caatttggat gttaaaagaa
     4201 tttaacaaag ataatgaaac agttatggtt tgccctgctg aaggattcta tgctactcca
     4261 ggtttaggac gtgatgaaat aagattagct tacatcttaa atgaaaaaga ccttcataga
     4321 gctgctacat tattaaaaga aggtttagag caatatgtag ctttacaaaa ataatttttt
     4381 aaacttaatt tatataaatt taatgatata aaatgtcccc ttttcagata ttatttgaaa
     4441 aggggacatt tcttaattaa tatattaatt aagacttaga cctgactatt tttcattttc
     4501 tttatatagt gttggccagt aatctttatt cactttaatt aaagcatcta aaacttgtct
     4561 tgccttagtt ggagagatta tagttctatt taatgttaaa gataaaatag ctttttcata
     4621 agattgttca aagtatgcat caactgctaa cttttcataa gctagttgac cttctaacag
     4681 tcccttataa aatgttggta tattacctac atgtaaaggt ctcggtccat tctttgtaag
     4741 aatcgcagct acttctacca ttgcatcatc aggtaagttg ctaatagccc cattattttc
     4801 tgttataact atggctattt ggtgtttgtt atgagctatt gcttctgcaa cttcaactat
     4861 aaattcccca tgagcatcat tatgcactaa attagaacct atagatgttc ctttttctat
     4921 aacagattta cattcttcaa atacacgctt ttctcttcca ttcataactt ctcttgctct
     4981 agtacaattt atatttgatt tagctacttt tttatcagga tatagataat actgtacata
     5041 tgtattaggt aagtaatctg gtacatcttc caacatatct ttaaccattc cataagtgtc
     5101 taaccaagat ttatctcttt gttctgcatc aacaggttgg aatccagagt ttgctactaa
     5161 atcttttatc ttaggtacca aatcttcacc atttttatca tacaaatgtg taaaccatcc
     5221 aaagtgattt aatccaaagt atacaggctc aaatgttctt gaatccatgt ctaacaatct
     5281 tccataagac cttaacaagt taactggttg gtcacacata ttaagtaatc ttttatcatc
     5341 tggaaatact ctttttaatg cttctgcaac aatagcagca ggatttgtat aatttaatat
     5401 ccatgcttta ggagagtatt ttcttacagt cttaaccatt tctatcatat cacctataga
     5461 ccttaaccca taagcaaatc caccagctcc acaagtttct tgaccaacca gtccaaattg
     5521 tagaggaatc ttttcatcta attctcgcat cttaagacca ccagttctca tttgaacaaa
     5581 tacaaaatct acatctataa atgcttcatc tgggtttgta gtatattcaa gtgttgcttt
     5641 aggatattct tctttaaata atatttttcc aaattcccct attaatcttt gacgttcttc
     5701 tactacatct aacatagtta ctttatttag aggaaatcta tcactcattt tacataaaga
     5761 ctttaaaagt cctggagtcc aagtactgcc tcctccaact atagctatat tgtatctttt
     5821 tttcattgtc atattaatca cccctgtttt ttattaacta acttgtaaga tgattataat
     5881 atataaagtt ttctacatga atacttttag gtaatcatta aaaatagttc aattttagta
     5941 tttttttcaa aattgtaatt tttttcatct attgtatttt tcaatgtatt tatgaaacat
     6001 atattccata agagaaagtg ctggcactct agatgtatag ttaacactat taaaattata
     6061 ttgtggagaa gatacaaata attttatatc agctatattt gataatgaat tgacacttat
     6121 atttgtcaga gaaattactt taacttgatt tgtttttgct atattacatg ccttaataac
     6181 ttgagcagtc tctccagaca tagatacagc aattacaagg tcatctttag taatatttga
     6241 tgcagccaaa tacatgagag ttgaatcatc atagtatttt acatttacac ctatagctga
     6301 aaaacgttga gacatttcag tacatactaa tctagaagac ccaacaccaa aaaaatatat
     6361 atgcttagaa ttatgtattt catttaaaca catatctata ttctctacat ttattaaatt
     6421 ttgtgtatcc ataacatcag taaaaatatc actttgactt ttagtaaact ccttatttct
     6481 ttcatttcta tttgaatatg aaaaaactaa aaagttttta aattcactat atccagaaaa
     6541 gccgagcttc tgacaaaacc ttactatgta tgcagtcgaa ttaaaagttt catttgctat
     6601 atctttaatc tttttttgat ttattttttc agaatttttt agaaaatagt ttaaaagaga
     6661 tatttcattt gtagataatt ttttttgatt tatatctaat ttgtctatta ggttcattta
     6721 tacctccata ataatgattt gtagttaaac aactttctca tatttattat ataaatatac
//
LOCUS       DAEPIN010000132         6686 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.134, whole genome shotgun sequence.
ACCESSION   DAEPIN010000132 DAEPIN010000000
VERSION     DAEPIN010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6686
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.134"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            390..1334
                     /locus_tag="KR250_003607"
     CDS             390..1334
                     /locus_tag="KR250_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HBH0069486.1"
                     /translation="MKFLRIEDEVIYSKKRYKLKREIDKLFLDEEYFQIEPQLFEEYD
                     EFTTINNKIPEESMVKVVNGKVMVLRADITTSIIKSLVPRWEDGLKLKLFYNSSIYKN
                     KNTVGIKEIRQIGCEYLGEASVEADREVVKLALKILEKYNNNFILEVGSSKYIHGLLE
                     ELNLNKNCENQIKNLLYTKNTHELKVYIEELKIKREVKELLSNILSLQGNLYNVIEKS
                     NKFYCNNKMKQALEELKQVNNLIEECNFLDKARFDLSMITMLDYYEGIMFRGYYPNSY
                     KEILSGGRYDSLTKEYGKEIPAIGFTLSVDELMKYVYK"
     gene            1356..1982
                     /locus_tag="KR250_003608"
     CDS             1356..1982
                     /locus_tag="KR250_003608"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HBH0069487.1"
                     /translation="MDYLTIALAKGRIEGESFKKFKKMGLGDSIDTDTRKLIFKDEEN
                     KIIYIHVKPSDVVTYVEKGVADLGIAGKDTILENETDVYEIYDLGFGKCKFAVAGLKG
                     DSIYREDEYLKVATKYPNIAKKYFKEKGQKIEIIKLNGSVELAPIVGLSDVIVDIVET
                     GNTLKANGLEILEDICNISARIISNRASYRFKYEQIQNIIRLFEELDN"
     gene            2016..3065
                     /gene="hisC"
                     /locus_tag="KR250_003609"
     CDS             2016..3065
                     /gene="hisC"
                     /locus_tag="KR250_003609"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HBH0069488.1"
                     /translation="MREKESIRELKGYEPNHVNCKVKLDANEGSKRLFKYLIKEISDS
                     DIDLNLYPEDSYSDLKESIIDYINISGVNKKNLLVGNGSSEIIDLIIHTFVDKDEVIL
                     SFSPSFSMYSIYSQINGSKFIGVESDENLVINIDNVIEKVKENNPKIVIVCNPNNPTG
                     TILKREEIIKLLDSTNSLVVLDEAYMDFGEESMLSDVFKYDNLIVLRTLSKAFGLAGI
                     RTGYMLSNSSLINSVEKVRPPYNLNSLSDFIATRALRNKDVVKAYIKEVKEEREVLYK
                     EMIGMGIKAYKSQANFILFYSEIENLSQKLIDRGVLIRKFGGKLENYYRVTIGDKEEN
                     SMFVGAIRDILKKEK"
     gene            3068..3646
                     /gene="hisB"
                     /locus_tag="KR250_003610"
     CDS             3068..3646
                     /gene="hisB"
                     /locus_tag="KR250_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HBH0069489.1"
                     /translation="MRIWKVERNTLETQILVELNIDGSGKAEIDTGIGFLDHMLTLMS
                     FHGKFDLKVICKGDTYVDDHHSVEDIGIAIGEAFKNALGDKKGIRRYSNIYIPMDESL
                     SMVAIDISNRPYLVFNAKFDTQMIGSMSTQCFKEFFRAFVNESRVTLHINLLYGENDH
                     HKIESIFKAFARALKEGSEIVSNEIASSKGVL"
     gene            3648..4253
                     /gene="hisH"
                     /locus_tag="KR250_003611"
     CDS             3648..4253
                     /gene="hisH"
                     /locus_tag="KR250_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HBH0069490.1"
                     /translation="MNIIVDYGLGNIDSVSRGFRKAGIETKISSDIDEIKQADSLILP
                     GVGAFRDSISALDKLGLIPIIKEHVSKGKFMIGICLGMQLLYEKSYEYGEYEGLGLIK
                     GSIDKLDISLKVPHMGWNNLKFNKANDDILKYINEDDYVYFVHSYYANSSNEELIAFS
                     EYEKKIPAIVRKGNVYGIQFHPEKSGEVGLNILRAYGEMIK"
     gene            4250..4972
                     /gene="hisA"
                     /locus_tag="KR250_003612"
     CDS             4250..4972
                     /gene="hisA"
                     /locus_tag="KR250_003612"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HBH0069491.1"
                     /translation="MIIFPAIDIKDNKCVRLTQGEFDKVNVYYDNPLEVAYKWKNEGA
                     EYIHIVDLNGARSEFGVNTKIIEDIANNIDIPIQVGGGVRDKEKVKSLINAGVTRVIL
                     GSIAIENLNLVEELVNEYKEKIVVSIDAKDGKVAVRGWEVVSNVDSLTLCKQLEKIGV
                     QTIVYTDISKDGMLQGPNFDIYERIAKETSLNVIASGGVTSIEDVKRLKAMNLYGAII
                     GKALYDKKIDFKEAQQLCLLGE"
     gene            4954..5712
                     /gene="hisF"
                     /locus_tag="KR250_003613"
     CDS             4954..5712
                     /gene="hisF"
                     /locus_tag="KR250_003613"
                     /EC_number="4.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000880088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HBH0069492.1"
                     /translation="MLTRRIIPCLDVRNGRVVKGKKFKDIVDVDSPEVLGKFYSDCGA
                     DELVFYDITASNEERKTSLEFVTKVAENINIPFCVGGGVNKLEDFTDILRKGADKVSI
                     NSSAVKNPELIREASLKFGAQCVVLSIDAKKNEEGSWSVYVKGGREKTNLDAIEWAVK
                     GVELGAGEIVVNSMDEDGMKNGYDIELLSKITSLVNVPVIASGGAGKKEDFYEAVNKS
                     NVDGILAASVFHFGEIKINDLKKYLKDMGVEVRL"
     gene            5740..6408
                     /locus_tag="KR250_003614"
     CDS             5740..6408
                     /locus_tag="KR250_003614"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HBH0069493.1"
                     /translation="MDNKCNNVYSDEVEKFIRSIKFDDKGLVPVVVQEVVSKDVLMLA
                     YMNKDAIKKTLKDKVACYFSRSRQELWVKGETSGNTQKVVKMSYDCDVDTILLFVEQT
                     GVACHTGNYSCFYRDLFDDSSKMELEVQTNILKELYDLINERKNNPVEGSYTNYLFEK
                     GIDKILKKVGEESSEVIIASKNTDKSELIYEISDLVYHTLVLMIEKGVEIDEIKKELL
                     KRRK"
BASE COUNT         2750 a          554 c         1280 g         2102 t
ORIGIN      
        1 ataaacaaaa gttacatata aaatatttta ttcaattttt taaataacta ttgccaattt
       61 ttgaaaaata tattattatt aatagtaaca aaaaactgta aatttagaat attaataaaa
      121 attatttggg aggaaattat catggtgaga ttaaatatag taaaattaaa tacaaaatta
      181 aatcatcacc atcatcatag attaaaggga ttgtaatatg aaattaattc atagacacaa
      241 gccctgtttt tagtgtaatt ggtcttgtat ctatgatggg aagtaatttt aagagaaaat
      301 tatagccata taggtataaa gcctatgtgg cttttttatt gttttaaatt tgcaaatata
      361 agagtaaatt taatgaagag ggtgagaatg tgaaattttt gaggattgaa gatgaggtta
      421 tttattctaa aaaaagatat aaattaaaaa gggaaataga taaacttttt ttggatgaag
      481 aatattttca aatagaacca caattatttg aagaatatga tgaatttaca actataaata
      541 ataaaattcc agaagaatct atggtaaagg ttgtaaatgg aaaagtaatg gtacttagag
      601 ctgatataac tacaagtata ataaaaagcc ttgtaccaag atgggaagat ggtttaaagc
      661 ttaagctttt ttataattca tcaatatata aaaataaaaa tacagttgga ataaaagaga
      721 taagacaaat aggttgtgag tatctaggag aagcctctgt agaagcagat agagaagtag
      781 tgaaattagc tcttaagata cttgaaaaat acaataataa ttttattcta gaggttggga
      841 gtagcaagta tattcatggt ttgttagaag aattaaattt aaataagaat tgtgaaaatc
      901 aaattaaaaa tttactatat acgaagaaca ctcatgaact aaaagtatat attgaagaat
      961 taaaaattaa gcgtgaagta aaagagttat tatcaaatat tttaagttta caagggaatt
     1021 tatataatgt tattgaaaaa tcaaacaagt tctattgtaa taataagatg aagcaagctc
     1081 ttgaagaatt aaagcaagta aataacttga ttgaggaatg taatttttta gataaggcta
     1141 gatttgattt atcaatgatt actatgcttg actattatga aggaattatg tttagaggat
     1201 actacccaaa ctcatataaa gaaattctta gtggtggaag atatgattct ttaactaaag
     1261 agtatggaaa agaaattcca gccattggat ttacactaag tgttgatgag ttaatgaagt
     1321 atgtatataa gtaaagttta gaaggaggga ttgctttgga ttatttgact atagcactag
     1381 caaaaggacg aattgaaggg gaatcattta aaaaatttaa aaaaatgggt ctaggagaca
     1441 gtatagacac agatacgaga aaactaattt ttaaagatga agaaaataaa attatatata
     1501 tacatgtaaa gccatcagat gtagttacat atgttgaaaa aggagtcgct gacctcggta
     1561 tagcaggtaa agatactatt ttagagaatg agacagatgt atatgaaata tatgatttag
     1621 gttttggaaa atgtaaattt gctgtagcag gattaaaggg agatagcata tatagagaag
     1681 atgaatattt aaaagtggca acaaagtatc ccaatatagc aaaaaagtat tttaaagaga
     1741 aaggtcaaaa aattgaaata ataaaattaa atggttctgt agaactagct ccaatagtag
     1801 ggttgtcaga tgtaattgta gatatagttg agactggcaa tacacttaaa gcaaatggtc
     1861 ttgaaatatt agaagacata tgtaatataa gtgctagaat tatatcaaat agagctagct
     1921 atagatttaa atatgaacaa attcagaata taattcgttt atttgaagaa cttgataatt
     1981 aaaatataat tgagctttat aaggagggat ttaagttgag agaaaaagaa agcataagag
     2041 agttaaaggg atatgaacca aatcacgtaa actgtaaagt aaaacttgat gcaaatgaag
     2101 gaagtaagag gttgtttaaa taccttatta aagaaatttc agatagtgat atagatttaa
     2161 atttatatcc tgaagattct tatagtgatt tgaaagaatc tataatagat tacataaata
     2221 tttctggagt aaataagaaa aatcttctag taggaaacgg ttcaagtgaa ataattgatt
     2281 taataataca tacttttgta gataaagatg aagtaatatt gagtttctct ccaagtttta
     2341 gtatgtattc tatatatagt cagataaatg ggtctaaatt tataggtgtt gaaagtgatg
     2401 aaaatttagt tataaatata gataatgtga ttgaaaaagt aaaggagaat aatcctaaaa
     2461 tagtaattgt atgtaatcct aataatccaa ctggaacaat attaaagagg gaagagataa
     2521 taaagctttt agattcaact aatagtctag tggttttaga tgaagcatat atggattttg
     2581 gagaagaaag catgttaagt gatgttttta agtatgataa tctaatagtt cttagaactt
     2641 tatcaaaggc atttggattg gctggaataa gaacaggata tatgttatct aatagtagtt
     2701 taataaattc tgttgagaaa gtaagacctc catataattt aaattcatta tcagacttta
     2761 ttgctacaag agcacttaga aataaagatg tggtaaaggc ttatataaag gaagtaaaag
     2821 aggaaagaga agttttatat aaagaaatga ttggtatggg aattaaagca tataaatcac
     2881 aagcaaattt tatattattt tattctgaaa tcgaaaattt atcacaaaaa cttattgata
     2941 gaggtgtttt aataagaaaa tttggtggaa agttagaaaa ttattacaga gttacaattg
     3001 gtgataaaga agaaaattct atgtttgttg gagctataag agatattttg aaaaaggaga
     3061 aataggtatg agaatttgga aggtagaaag aaatactctt gaaacacaga ttttagtaga
     3121 attaaatatt gatggttctg gtaaagctga aatagatact ggaataggat ttttagacca
     3181 tatgcttacc ttaatgtcat ttcatggcaa gttcgattta aaagtgattt gtaagggtga
     3241 tacatatgta gatgaccacc atagtgtaga agatataggt atagctatag gtgaagcatt
     3301 taaaaatgct ctaggtgaca aaaaaggaat acgaagatat tctaatatct atatacctat
     3361 ggatgaatct ctatcaatgg tagcaataga tataagcaat agaccttatc ttgtatttaa
     3421 tgctaaattt gatacccaaa tgattggtag tatgagtact caatgcttta aagaattttt
     3481 tagagctttt gtaaatgaat cgagagtaac attacatatc aatctcttat atggggaaaa
     3541 tgaccatcat aagatagaaa gtatttttaa agcatttgca agagctttaa aagagggtag
     3601 tgaaatagtt tctaatgaga tagcatcttc aaagggggtt ttgtagaatg aatataatag
     3661 tagactatgg tcttggaaat atagattctg tgtctagggg ttttagaaaa gctggaatag
     3721 aaactaaaat ttctagtgat atagatgaaa taaaacaggc agattcactt atacttccag
     3781 gtgttggagc ttttagagat tctataagtg ccttagataa actaggattg ataccaataa
     3841 taaaggagca tgtttctaag ggtaaattta tgataggtat atgtctagga atgcaattac
     3901 tttatgaaaa aagttatgag tatggagaat atgaaggatt aggacttata aaaggaagta
     3961 ttgataaact ggatataagt ttaaaagtgc cacatatggg atggaataat ctaaaattta
     4021 ataaggcaaa tgatgatata cttaaatata taaatgaaga tgattatgta tacttcgttc
     4081 actcatatta tgcaaattca tcaaatgaag aactaattgc attttcagaa tatgaaaaga
     4141 aaatccctgc tattgttaga aaaggcaatg tatatggtat acaatttcat cctgaaaaaa
     4201 gtggagaagt agggttaaat atattaagag catatgggga gatgataaaa tgataatatt
     4261 tccagcaata gatataaaag ataataagtg tgtaagactt acacaaggag aatttgacaa
     4321 agtaaatgta tattatgata atccattaga agtagcatat aaatggaaaa atgaaggagc
     4381 agaatatata catatagtag atttaaatgg tgctagaagt gaatttggtg taaatacaaa
     4441 aataattgag gacatagcaa acaatataga tatacctatt caagttggcg gtggagttag
     4501 ggataaggaa aaagtcaaaa gtttaataaa tgctggtgtt actagagtaa tattaggtag
     4561 tatagcgatt gaaaatttga atttagttga agaattggtc aatgaatata aagagaaaat
     4621 tgtagtttct atagatgcta aagatggaaa agtagcagtt aggggatggg aagttgtaag
     4681 caatgtagac tcattaacat tatgcaaaca acttgaaaag ataggtgtac aaactattgt
     4741 ttatacagat atatcaaaag atgggatgtt gcaaggtcca aattttgaca tatatgaaag
     4801 aatagcaaag gaaacatcac taaatgttat tgcttctgga ggcgtaactt ctatagaaga
     4861 cgtaaaaaga ctaaaagcta tgaacttata tggtgcaata ataggtaaag cgctttatga
     4921 taaaaagata gactttaagg aggcacaaca attatgctta ctaggagaat aataccttgt
     4981 ctagatgtga gaaatggaag agtcgtaaag ggaaaaaaat ttaaagatat agtagatgtt
     5041 gatagtccag aagtacttgg aaaattttat agtgattgtg gtgcagatga acttgtattc
     5101 tatgatataa ctgcatcaaa tgaagagaga aagacatctt tagaatttgt gacaaaagtt
     5161 gcagaaaata taaatatacc attttgtgtt ggtggaggag taaataaatt agaagatttc
     5221 actgatattt taagaaaagg tgctgataaa gtttctataa attcatcagc agtcaaaaat
     5281 cctgaattaa taagggaagc ttcattaaaa tttggagcac aatgtgttgt tttatcgata
     5341 gatgcaaaga aaaatgaaga gggttcatgg agcgtatacg taaaaggtgg aagagaaaaa
     5401 actaatctgg atgcaattga atgggctgtt aaaggtgtag aacttggagc aggagaaatc
     5461 gttgtaaata gtatggatga agatggaatg aaaaatggat atgatataga attattatca
     5521 aagataactt ctttagtaaa tgtacctgtc atagcatctg gaggcgctgg aaaaaaggaa
     5581 gatttttatg aggcggttaa taaatccaat gtagatggaa ttttagcagc atctgttttt
     5641 cattttggcg agataaaaat aaacgattta aagaaatatt taaaagacat gggtgtagaa
     5701 gtaagattat aagttaaata ctaaaaggag attagaaata tggataataa gtgtaacaat
     5761 gtatacagtg atgaagtaga gaaatttata agaagtataa agtttgatga taaagggtta
     5821 gtccctgttg ttgttcaaga ggtagttagc aaagatgtac tgatgttagc atatatgaat
     5881 aaagatgcta taaagaaaac cttaaaagat aaagtagctt gctattttag tagaagtaga
     5941 caagaacttt gggttaaagg tgagacatct ggtaatactc aaaaagtagt taagatgtct
     6001 tatgattgtg atgtagatac tatactactt tttgttgaac aaactggtgt agcttgtcat
     6061 acaggcaatt attcttgttt ttacagagac ttgtttgatg atagttcaaa aatggagctt
     6121 gaagtacaaa caaatatatt gaaagaatta tatgatttaa tcaatgaaag aaaaaataat
     6181 ccagttgaag gttcttatac taattatctt tttgaaaaag gaatagataa gattcttaaa
     6241 aaagtaggtg aagagagtag tgaggtaatt atagcatcaa aaaataccga taaaagtgaa
     6301 cttatttacg aaattagcga tttagtatat catacattgg ttttaatgat agaaaaaggt
     6361 gttgaaattg atgaaataaa aaaagaatta cttaaaagaa gaaaatagaa taagatagat
     6421 taatgggtaa taagaaaata taccactttt aataaaaagt ggtatatttt ttattttata
     6481 ttttgattta tagaataata tttaaattac ttgataaaat taactatata tataatttat
     6541 tgataaaata attctagtac attatttatt ttagaaaaaa gataattatc taattatatt
     6601 taaggataat aatatgaaaa taatttttat attaaaaaat atgaatgaaa gtagtgggtt
     6661 ttataaaaaa acatatttag atttta
//
LOCUS       DAEPIN010000133         6584 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.135, whole genome shotgun sequence.
ACCESSION   DAEPIN010000133 DAEPIN010000000
VERSION     DAEPIN010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6584
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.135"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            complement(619..1179)
                     /locus_tag="KR250_003615"
     CDS             complement(619..1179)
                     /locus_tag="KR250_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HBH0069494.1"
                     /translation="MLKKLKKMLLITCCLFMMSSTAFAETQNSEGKSFTISGYTFMYE
                     NAPQEIKDKYKENCLDIGVEPQPNDEIFITPSELKTYSNIDMYSARSSSFTVTYHYSY
                     YKVTGDKNYTVNTSTIVKYGNSGNPVHLVQLLLKKVGYALEVDSQFGNTTLSKVKSFQ
                     GSYGLTVDGIVGSATWKKFASATNLD"
     gene            complement(1225..2208)
                     /locus_tag="KR250_003616"
     CDS             complement(1225..2208)
                     /locus_tag="KR250_003616"
                     /inference="COORDINATES: protein motif:HMM:NF017390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M56"
                     /protein_id="HBH0069495.1"
                     /translation="MSNLILTDILRTSITSSLVICFLLLLRTTIFRIFNKGFNYYIWL
                     IVIVRLLFPFSYFTYTISDSKFQYKPILNEVDYSNTVLIIYLTIVILLAIYRFIKYLK
                     FKSLVIDLSYDIENLEINNLYNNLLKELNIEKHIKLKYTYEINTPSFFGLFDNYILLP
                     PHNYNLNELHWILKHELIHYKSKDLYIRYLVLFLKCVYFFNPFIYLLDRIIYHSCELH
                     CDEVVLKGCSLENIKSYANTILDSIERSSRDSNNYTTGLHRKSNFEKRVYDMFKNKGK
                     SGILIALILCLLSSFTYFKFDSIISKPINHSLDAPLPVNTGKEKSFTITID"
     gene            complement(2221..2583)
                     /locus_tag="KR250_003617"
     CDS             complement(2221..2583)
                     /locus_tag="KR250_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBH0069496.1"
                     /translation="MIEKIPRAELKVMMLFWDTDKVLTSRDIVNIMSEEWKQTTTLTL
                     LSRLSKRGFLEPTKKPTHTTYKILIDKKSYLEFATSDFVKNVHNDSIVSLVDSVNKEK
                     IGKDELASLRAFIKKLKK"
     gene            3826..4473
                     /locus_tag="KR250_003618"
     CDS             3826..4473
                     /locus_tag="KR250_003618"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069497.1"
                     /translation="MNKKLEKPFVYKREYDLTGYDVEILQKYELEQAIYVYVGSSCAY
                     NMRARSSKWRYHIRTNNKSICCNIKNFIHNLELFYKMELKLSDNIINDKLYYSNIAEF
                     EEFETLEKAREVESTIISQYQFLDSINHMLKQKIILLSNKDSVLNITKNGNTNYLKVK
                     NKYIEKHKNKPIMRYHINCQFNTDGSVKSITQEFEPILELNKKNTLSRPSRVFLK"
     gene            4748..6385
                     /locus_tag="KR250_003619"
     CDS             4748..6385
                     /locus_tag="KR250_003619"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069498.1"
                     /translation="MEQLDSKYKLKKFLMAVFRDGIGQGNNLIDNEYVRVFQNNKSNS
                     KQLELGEEFKEYSKTTFFKNIDDIVEFTFAKNIYYENTFFNLCTTDGKAGTNENLINR
                     YALGFDFDKKELGQGFNYKDIINLFTKIGLHYHILVDSGNGFHVYVLINKTNNIKLVS
                     EVTNTLINKLGADKQANLSTQVLRVPYTYNIKNTTKQVKIIHQDKNIYRYDIEKLAKK
                     YCKDVKTVGNTNTKYILDSKLPNCIVDILKNGSKDGHKNLDLQKIVVTLRLRNKSLSQ
                     VISVAREWNYISQNSLSNSELEYQVKYMYEKLKTVNFGCTGCEFNSDCWNKIESDFIY
                     SDEDTLFNMPHKHSKDLKYKNRKGVKIMTGNQLFIYNVLLNNKDRELNIDDIMELITY
                     KRKKKVKNIVMSEKTLRETLKELQHNDYITKTKGVTKLGIKDTYNVKEVRCNIDKQYT
                     ISYFVTMAVIWGIISTEELRLYTHMRYKQDLLVKDDKIKGNILRINQEELAKDLGVTQ
                     QRISNMIESLLDTKILDVWETKINDRGFMYYTYRLNK"
BASE COUNT         2683 a          692 c          917 g         2292 t
ORIGIN      
        1 ctaattagta ggtgcttttt taaaacaagt taaaaatcaa aaatagtatt agtaagcatt
       61 ggaaatgcta gattctaaaa tagaaaagta aaaaattggt gcactatcta aacttatcta
      121 tatcgctttt tccgtcgttt ggttctctag ttacgataca ggggatatgc ttatattgag
      181 ttatagtact aatcagtgct taatatagtt aataaaatta tagttaccat agtttagtaa
      241 ctatgatgta tgttagttag aaacttgcat ttcatgagtt gtaatatctt aaagtcagta
      301 gttttgattt taaaatatta attgcactgt tacaaatgac tttattaata aaaaattagg
      361 cagcttaaaa aaagtttaga ataaatttgc taggctttgc taagctattc ttttaaaaat
      421 aactttacaa aacaaacaaa taaggactta taaatataga taaaaaaaga aaattgaaaa
      481 ggattataga gacttacaag gctttaaata tatattctta attgtaaaat tatgtaatta
      541 aaatatttac tttattataa aaagacatga taaacttatt atcatgtctt ttgttaaact
      601 tatttatatg taaaaattct aatcaaggtt tgtagcagat gcaaattttt tccatgttgc
      661 actaccaaca attccatcta ccgttaagcc ataactgcct tgaaaacttt taactttgct
      721 aagtgtagtg ttcccaaatt gactgtctac ttctaaagca tatccaactt tttttaatag
      781 caattgtact aaatgaacag gatttcctga atttccgtat tttacaatcg tagaagtgtt
      841 gacagtatag tttttatctc cagtaacttt gtagtatgaa taatgataag ttactgtaaa
      901 tgaacttgac ctagctgaat acatgtctat gttgctataa gtttttaatt cactaggggt
      961 aataaaaatt tcatcattag gttgtggctc tacacctata tccaaacaat tttccttata
     1021 tttatctttt atttcttgtg gtgcattttc atacataaat gtgtaacccg aaatagtaaa
     1081 gctttttcct tcactatttt gtgtttctgc aaaagctgtg ctactcatca taaataaaca
     1141 acaagtaatt agcaacattt tttttaattt ttttaacata attcctccct ataattttga
     1201 atatatttga ataccagcaa atatttaatc tattgttata gtaaaagatt tttctttgcc
     1261 tgtattaact ggcaatggtg catctaagga gtgattaatt ggctttgata taattgaatc
     1321 aaatttaaaa tatgtaaatg atgacaataa gcataaaatc aatgctatta aaataccact
     1381 cttaccctta tttttaaaca tgtcataaac tcttttttca aaatttgatt ttctgtgtaa
     1441 tccagtagtg tagttatttg aatctcttga acttctttca attgaatcta aaattgtgtt
     1501 agcataactt tttatatttt caagggaaca accttttaat acaacttcat cacaatgtaa
     1561 ttcacagcta tgatatataa ttctatcaag tagataaata aaagggttaa agaaataaac
     1621 acactttaaa aataaaacca aatatctaat gtataaatct ttacttttgt aatgaattaa
     1681 ttcatgtttt agaatccaat gcagttcatt taaattataa ttgtgtggtg gcaaaagtat
     1741 ataattatca aataatccaa aaaatgaggg cgtattgatt tcataagtat attttagttt
     1801 aatgtgcttt tctatattta gttcttttaa taaattatta taaagattgt ttatttcaag
     1861 attttcaatg tcatatgata agtcaataac caaactctta aactttaaat attttataaa
     1921 cctataaatt gcaagtaaaa taacaattgt tagatatatt attaatactg tatttgaata
     1981 atcaacttca ttcaaaatag gtttgtattg aaatttagaa tcagaaatag tataagtaaa
     2041 ataagagaat ggaaaaagta atctaactat tacaattagc caaatataat aattaaaacc
     2101 cttattgaaa attctgaata tagttgtcct taaaagtagc agaaaacaaa taactagact
     2161 acttgtgata ctggttctta aaatatctgt tagaattaaa ttagacatat atagtaccta
     2221 ttatttctta agttttttta taaaagctct caaagatgcc aattcatctt ttcctatctt
     2281 ttctttatta actgaatcaa ctaaacttac aatagaatca ttatgtacat tctttacaaa
     2341 atcagatgtt gcaaattcta agtagctctt tttatcaatc aaaattttat aagtagtgtg
     2401 tgtaggtttt tttgtaggct ctaaaaaccc tcttttagaa agcctagata atagtgtaag
     2461 tgttgtcgtt tgtttccatt cttcactcat tatatttaca atatctcttg atgtcaatac
     2521 cttgtcagta tcccaaaata gcatcattac tttaagttca gcacgtggta ttttttctat
     2581 cataatatgt tcctccatcc taatgttaaa aaaattaata aatgtaattt tattctattg
     2641 aaatagagta ggacagtcaa ttgttttttg attaatgatt tacatatgta gagtaaatta
     2701 aaagaagtat atcacttgaa aagttttaca tacgtagagc atttttatca aatctcttaa
     2761 tttttttaat tgaattatat atgtgaatat aagagtaaat caacttaaaa catgcaaaaa
     2821 attaatattt actgacatgc aactgacaaa tcaacaaaaa taggaattga taaaataagt
     2881 gtatatattg aaattgctat aatacataca aaaatggcat aaaatactaa tttttatgtc
     2941 cttttctact gacatgtaaa aaagtcttgt aagtattgga attactagat tacataggtt
     3001 ttagatgcaa aattgtcgtt ttgtaaaaaa ggctactaaa atttataaaa acaattatta
     3061 attgatataa attatcacaa aaaaagacat ctatttctat gaaaaaaaaa ttttcgtctg
     3121 catgtaaaaa tagctatagt aatgcgtacg aatgttctat tattacagaa ttatttgtat
     3181 gtaaattata ttgaaaatat ctatcagaat tagaatttta tatggataaa acagaactat
     3241 caaataggag tatatagtaa agatttatat aatatgatga cctcgtacaa gcctgtaaga
     3301 gccgtttaaa agttgcttta gtattaatta tctttgaaaa tttaaaatag gtttaaatgg
     3361 cttgttaaat atgatgtatt actattatgt ataggggggt agttatgtag ttacatgttg
     3421 ttttaaaaaa taaatttaac tatagttctt tcacttccac attacaaaaa ataaattgag
     3481 tgtgtatgtt tttaagtaaa aatgatgttt aaatattgaa aaatagagat atagtgtatg
     3541 taaaatttag aatagtgtat gtaattttag gcataaaaat atgtgattaa accttaaaaa
     3601 ccaggaatat agtgtatgca tttttaaaaa taaaaattgt aacctttttg taatctttta
     3661 ttaaaatact tactttaagt ctaaaaatta ggggagatgt aaggatttgg gaaaaataga
     3721 agatgttata atcataaata tggtattcgt aggcttaaag tcaaaaagga ggtgaaatat
     3781 aaatagattt ttagctaaat taagtaagaa ataggaggag atttattgaa caaaaaatta
     3841 gaaaaaccat ttgtatataa gagagagtac gatttgactg gatatgatgt tgaaatttta
     3901 caaaaatatg agttagaaca agcaatatat gtttatgttg ggagtagttg tgcatataac
     3961 atgagagcta gaagtagtaa atggagatac catataagaa caaataataa gtctatatgt
     4021 tgtaacatta aaaattttat acataacttg gaattgtttt ataaaatgga attaaagttg
     4081 tcagataata ttattaatga taagctatac tatagcaata tagcagagtt tgaagaattt
     4141 gaaacactag aaaaagctag agaggtagaa agtactatta taagtcaata tcaattttta
     4201 gattctataa atcatatgtt aaaacaaaaa ataattttat tgagtaataa ggatagtgtg
     4261 ttaaacataa ctaaaaatgg aaatacaaat tatttgaaag taaaaaataa atacatagaa
     4321 aaacataaga acaagccaat aatgagatac catatcaact gtcaattcaa tacagatgga
     4381 agtgtcaaaa gtattacaca ggagtttgaa ccaatattgg aattaaacaa aaaaaatacc
     4441 ctaagccgac caagcagagt atttttaaaa taatatttta agataacaac aaaatgagat
     4501 aatactacta gacaatgaca actcaactac caattgagtt tatggagcta ccaactccaa
     4561 tatcggtcta actgattaag tatctgtagt tatataataa tattgctatc aattttagca
     4621 tcttaacaat attattatac atactaagct aaaattattc aatagttgta aaagttgatt
     4681 agtcaataag tatatattta atgtagtgtt atctcttaaa aaaactagat aaggagataa
     4741 taaatatatg gaacaattag attcaaaata taagttgaaa aaatttctaa tggcagtatt
     4801 tagagatggt ataggacaag gaaataatct tattgataat gaatatgtta gagtatttca
     4861 aaataataaa agtaatagta aacaattaga actcggagaa gaatttaaag aatatagtaa
     4921 aacaactttt tttaaaaata tagatgatat agtagaattt accttcgcaa aaaatattta
     4981 ttatgaaaat acatttttta acctatgtac aactgatgga aaagcaggaa ccaatgaaaa
     5041 cttaataaat agatatgcat taggatttga ttttgacaaa aaagaattag gacaaggttt
     5101 taattataaa gatataatta atttatttac taagatagga ttacattatc atatcctagt
     5161 tgatagtgga aatggattcc atgtttatgt gctaattaat aaaactaata acattaagtt
     5221 agtatcagaa gttacaaata cattaataaa taaattgggt gcagataaac aagcaaattt
     5281 atctactcaa gtattaagag taccttatac atataatatt aaaaatacta ctaaacaagt
     5341 aaaaataata caccaagaca aaaatatata tagatatgac atagaaaagt tagctaaaaa
     5401 atattgcaaa gatgtaaaaa cagtaggtaa tactaataca aaatatatat tagatagtaa
     5461 gctaccaaat tgtatagtag atattttaaa aaatggtagt aaagatggac ataaaaacct
     5521 agatttgcaa aaaatagttg tgactttaag attgaggaat aaaagtttaa gtcaagtaat
     5581 atccgttgct agagaatgga actatatatc acaaaatagt ctttcaaata gtgagctaga
     5641 atatcaagtc aagtatatgt atgagaagct taaaacggtt aattttggtt gtacaggttg
     5701 tgagtttaat agtgattgtt ggaataaaat agaatcagat tttatatata gtgatgaaga
     5761 tactttgttc aatatgccac ataagcactc aaaggatttg aaatataaga ataggaaagg
     5821 ggttaaaata atgactggta atcaattgtt tatctataat gtgttactta acaataaaga
     5881 tagagaatta aacatagacg atataatgga gctgataacc tataaacgta agaagaaagt
     5941 taaaaacatt gttatgagtg aaaagacatt aagagaaaca ttaaaagaac ttcaacataa
     6001 tgattatatt acaaaaacaa aaggtgttac aaagctagga ataaaagata catacaatgt
     6061 aaaagaagtt agatgtaata tagataaaca atatactatt agttactttg ttaccatggc
     6121 agtaatttgg ggaataattt caactgaaga attaagatta tatactcata tgagatataa
     6181 gcaagattta ttggtcaaag atgataaaat aaaaggaaat atattaagaa ttaatcaaga
     6241 ggaattagca aaagatttag gagtaacaca gcaaagaatt tcaaatatga tagaatcttt
     6301 attagatact aaaattttag atgtatggga aactaaaata aatgatagag gatttatgta
     6361 ctatacatat agattaaaca agtagatttt tgataggatt agaattgatt ttctagtcct
     6421 atttttatgc aaaaaaacta attaataaaa atttcttttg gtaaaataat tgtacgagaa
     6481 ttgcaaaaaa aaaatggcat caaagtattg aaattaagcc gttttaaaaa tttcttttgg
     6541 taaaataatt gtacatatat atgtagtata tatatatatg tttt
//
LOCUS       DAEPIN010000134         6536 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.136, whole genome shotgun sequence.
ACCESSION   DAEPIN010000134 DAEPIN010000000
VERSION     DAEPIN010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6536
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.136"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            349..1623
                     /locus_tag="KR250_003620"
     CDS             349..1623
                     /locus_tag="KR250_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HBH0069499.1"
                     /translation="MQFMDVSKYIEGANLKVKTKNGETVKRIYFNNSATPLVLKNVVD
                     NLNCEIPWLTYINAPGIISEKNTLKYENVRSTILKLIDGDEEKDSVIYVKNATEGINF
                     LAKFFKDENPDKFVITTAMEHMANYLPFKVNFPTKVVGITEKGELDLCQLENILKNYA
                     GNVSLVTVTGASNVTGITTPIYEIARIAHKYGAKILVDIVQVIQHKPFSMMPHECDEH
                     IDFVVFSAHKCYSPLNGGALVGPKKFFEKFNPVLYGSGSTKFVNDNKILYANVPQRFE
                     AGYPEYFGTLAMGKALNTLNKIGLNRISKYERELFLYAKEEMSKIPNIIIYGDKSNNV
                     IIPNISFNISNMYYKDVAKYLVDNFGIETGAGVVGADIYVQKLLGINPKEAYVRFMRE
                     NPVGLVRISLGMYNTFDEVDSFIHAIKTLAFK"
     gene            complement(1830..2147)
                     /gene="trxA"
                     /locus_tag="KR250_003621"
     CDS             complement(1830..2147)
                     /gene="trxA"
                     /locus_tag="KR250_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HBH0069500.1"
                     /translation="MVKIINNNEFINEVENKDGLVVVDFFATWCGPCKMLSPIYEALG
                     NEMVEKANFLKVDIDQSIELAQKFEVSTVPTMLIFKDGKPVDRLIGFMPKENLKNKIE
                     SYM"
     gene            2414..3166
                     /locus_tag="KR250_003622"
     CDS             2414..3166
                     /locus_tag="KR250_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HBH0069501.1"
                     /translation="MDNIINELQNIGFTKYESQIYISLLKESPLTGYEISKLSGVPQS
                     KVYENITKLLNNNIIINVGTDPIKYIPINPNELLKNKKKEFDNSMDNLKNSLQILNKS
                     KSVEYVLNIKGDKNILKKAIEMIKNAKQEIIISCPYEEILELKKELLIAYKKNINIEI
                     LLLENKSLKGLDNIHIHGGNKTTLENKMLYKGIILIVDNDEILTGNLSEGSEAISIWT
                     KNSNILYIGVQYIKHEIYISEQIKGGYKNEQN"
     gene            3153..4034
                     /locus_tag="KR250_003623"
     CDS             3153..4034
                     /locus_tag="KR250_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HBH0069502.1"
                     /translation="MNKIKIKQVDAFTTIPFGGNPAGVVTDASNISDEIKQNVAKEMN
                     LSETAFVSESNIADFKVQFFTPGLEVDLCGHATIGTFHALLEEGKLNTNKSIFYQETK
                     AGVLSVELKKINNENIFMMEQNTPEFENLDNCRKELAQMIGVHEDRLLEYPIIKVNTG
                     LWWLVFGLKSLDDLKNISPDLNKIKKFSGDNNIIGITPFCIETIDKKCDYHLRSIAPY
                     VGVTEDPVCGTGNACVSSYIVHHNILDKTSFIGEQGNFINRPGKVYVNITRENNDITS
                     VKIGGSAYTILSGEIMY"
     gene            complement(4260..5615)
                     /locus_tag="KR250_003624"
     CDS             complement(4260..5615)
                     /locus_tag="KR250_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HBH0069503.1"
                     /translation="MSSTVKKKGKFPMGFYICSTTFSFERAAYYASKYLIYIFLTTAI
                     VHGGLGIDKGQAAIMQANLVAFTYLAPIIGGYISDRWIGARYTIPIGMLIMGVGYYLG
                     SIATTVSMVNAMIILVSIGTAFFKGNVSAVNGQLFDSQEELDTAFSVQYSFVNIGSFI
                     GTIAVGILYLKTFAKNGVLGFSQCFFIAAVLCVIGAIWFIYGWRFLGNAGKRPFKEGV
                     VAEKIEEKDKSPLTSMDKKRIWAIILISFFSVIFWVFWYLTYLAVYDYGAAFVNMNVG
                     GFDVPLAWFDSLNSLVCIVLGPVLGALWFKLASRPQGDMSLFKKTGLGLIFLGLAFLM
                     LVGAEFSRGVGAPETAKASILWIIMFGILLSLGEMLFSPLGNSFVSKYAPKKLLGVLM
                     GVWTFATFIAGKGYGYIYAFTLKFDMIKVYTIIPVILFVAAILLFLSDKTLSRLVEDD
                     K"
BASE COUNT         2514 a          939 c          826 g         2257 t
ORIGIN      
        1 atttctttac tatttttaca actttattag tcatattcat aattatttct aattagtgtt
       61 taacatttta tgtaaaattt ataaaaaaat gtatataaat taaaataagt gactatacta
      121 aaaatatgat acggataaac aataagttca acctatctcc cccaaaatca agtgagctac
      181 agtttccccc aactgtagct cattttcgtg taatttaata aacatctcaa caagaattaa
      241 ttaacaaaac cccttataaa aacataacat atattaaaca agctcttgat taaaacttga
      301 gcttttttaa taaaaaacag aatagtttat tagataaggg gtttttttat gcaattcatg
      361 gatgtatcta aatacattga aggtgcaaac cttaaagtta aaactaaaaa tggtgaaact
      421 gttaaaagaa tatattttaa caactctgcc actccattag ttttaaaaaa cgtagtggat
      481 aacttaaact gtgagattcc ttggcttact tatattaatg ctccaggaat aatctcagaa
      541 aaaaatactt taaaatatga aaatgtacgc tctactatcc tcaaacttat tgatggagac
      601 gaagaaaagg attctgttat ttatgtcaaa aatgctacag aaggaataaa cttcttagca
      661 aaatttttta aagatgaaaa tccagataaa tttgttatta ccacagcaat ggagcatatg
      721 gctaattacc tgccttttaa agttaatttt cctacaaaag ttgttggaat tacagaaaaa
      781 ggagaacttg atttatgtca attagagaat atacttaaaa attatgctgg taatgtatct
      841 ctcgttacag ttacaggagc tagtaatgtc acaggtataa ccactcctat atatgaaata
      901 gcaagaatag ctcataagta tggtgcaaag atattggtgg atattgtgca agtaattcag
      961 cataaacctt tttcaatgat gccacatgaa tgtgatgagc atatagattt tgttgttttt
     1021 agtgctcata aatgttattc tccactaaat ggaggagctc tggtaggacc taaaaaattc
     1081 tttgaaaaat ttaatcctgt actatatggc tcaggttcta ccaaatttgt aaatgataac
     1141 aaaatactat atgcaaacgt accccaacgt tttgaagcag gatacccaga gtattttgga
     1201 actcttgcaa tgggaaaagc actgaacact ttaaataaaa ttggtctaaa cagaatttca
     1261 aaatacgaaa gagaattatt tttatatgct aaagaagaaa tgagtaaaat acctaatata
     1321 ataatatatg gagataaatc aaataatgta attatcccaa atatatcttt taatataagt
     1381 aatatgtatt acaaagatgt agcaaaatat ttagttgata actttggaat agaaactgga
     1441 gctggagtag taggtgcaga tatttatgta caaaaattat taggtataaa tccaaaagag
     1501 gcatacgtca gatttatgag ggaaaatcct gtaggtcttg ttaggataag cttaggaatg
     1561 tacaatactt ttgatgaggt ggatagcttt atacatgcta ttaaaacttt ggcttttaaa
     1621 taagattttt ctattctttt ttatcaataa atagagagta gttttgtttt cacaaaaaaa
     1681 caaattatat caacagtcaa tttcattagc aattagacac tcatttgtaa ctatacaaac
     1741 tattttacac tatacaaaca aaaaactcat tacaatgagt ttttttgttt atatatgaga
     1801 tttattttaa ttacttcaat ttactttaat tacatatatg attcaatttt gttttttaaa
     1861 ttctccttag gcataaaacc tattaatcta tctacaggtt ttccatcctt aaatattagc
     1921 atagttggta ctgtagatac ttcaaatttc tgagctaatt ctatactttg gtctatgtcc
     1981 accttcaaga agtttgcttt ttcaaccatc tcatttccta aagcttcata tattggtgat
     2041 agcatcttac aaggtccaca ccaagtcgca aagaaatcta caactactaa accatcttta
     2101 ttttctactt catttataaa ttcattatta tttattattt ttaccataat atacatctcc
     2161 tttattatct tcttgatatt aatatactac gccctatata acagttcaat taggcacttt
     2221 tttgtaaccg cctattttta atagatttaa tattatgata aatagattta taagattaaa
     2281 tatttatgag actaaatatt tctaatatat tttatttttt tcttaatata gttgacattt
     2341 tttcccttaa attataaaat caaattaata actacatagg tagttactaa tcttttatca
     2401 aaggagtttc tatatggata atataattaa tgaattacaa aatataggat ttactaaata
     2461 cgaatctcaa atatacatat cattattaaa ggaaagccct ttaacaggat atgaaataag
     2521 taaattatca ggtgtaccac aatcaaaagt ctatgaaaat ataacaaagc tattaaacaa
     2581 taatataatt atcaatgttg gaactgaccc tattaagtac ataccaataa accctaatga
     2641 attattaaaa aataagaaaa aagaatttga caattctatg gataatctaa agaatagctt
     2701 acagatatta aataaaagta aaagtgtaga atacgtatta aacataaaag gagataaaaa
     2761 tatattaaaa aaagcaatag aaatgataaa aaatgcaaaa caagaaatta taatttcttg
     2821 cccttatgaa gaaattcttg aattaaaaaa agaactccta attgcttaca aaaaaaatat
     2881 taatattgaa atactacttt tagaaaacaa aagtctgaaa ggtcttgata atattcatat
     2941 tcatggaggt aataaaacta cacttgaaaa taaaatgctt tacaaaggaa ttattttgat
     3001 tgtagataat gatgaaatat tgacaggaaa cttatcagaa ggcagtgagg ccatttcaat
     3061 ttggacaaaa aactctaaca ttttatatat tggagttcaa tacataaaac atgaaattta
     3121 tatttcagaa caaataaaag gaggttataa aaatgaacaa aattaagatt aaacaagttg
     3181 atgcttttac aactatacct tttggtggaa atcctgctgg tgtcgttact gatgcctcta
     3241 acatttctga tgaaattaag cagaatgtgg ctaaagaaat gaatttatca gaaactgcat
     3301 ttgtttcaga gtcaaatatt gcagatttta aagtacaatt tttcacacca ggtttagaag
     3361 ttgatttatg tggtcatgca acaataggta ctttccatgc acttttggaa gaagggaagc
     3421 ttaatactaa taagagtata ttttatcaag aaacaaaagc tggtgtatta tctgtagagc
     3481 ttaagaaaat aaataatgaa aatatattta tgatggaaca gaatacacct gaatttgaaa
     3541 atttagataa ttgcagaaaa gaacttgcac aaatgatagg tgtgcatgaa gatagacttc
     3601 ttgaatatcc tattataaag gtaaatactg gactttggtg gcttgtattt ggtctaaaat
     3661 ctttagatga tttaaaaaat atctctccag atttaaataa aatcaaaaaa tttagtggtg
     3721 ataataatat aattggtata actccatttt gtatagaaac tatagataaa aagtgtgact
     3781 accatttaag atctatagca ccatatgttg gagttacaga agaccctgta tgtggaactg
     3841 ggaacgcttg cgtatcatct tatatagtac atcacaatat tttagataaa actagtttta
     3901 ttggtgagca aggtaacttt ataaacagac ctggaaaggt atatgtaaat ataactagag
     3961 aaaacaatga cataacttct gtaaaaattg gaggaagtgc ttatactatt ttaagtggtg
     4021 aaattatgta ttaatcccat acctaataaa aacaaataaa aatactagct aactaaagct
     4081 agtattttta tttgttattt ttatttaata atcatactct aacaaatatt ataattatat
     4141 atatcataat attgtattta gaagtaaaat tttattttta ttattaatta gttataaatc
     4201 aatctaatac aacataatta cagtagatta acctataaaa cataataatt gcttactaat
     4261 tatttatcat cctcaacaag tctacttaat gtcttatcag ataagaataa taatatcgct
     4321 gcaacaaata gtattactgg aattatagta tatactttaa tcatatcaaa tttaagagta
     4381 aatgcatata tatatccgta tcctttacca gctataaaag tagcaaatgt ccatactccc
     4441 ataagaacac ctagaagctt cttaggtgca tacttactta caaaagagtt accaagtggt
     4501 gagaataaca tttcaccaag acttaaaaga ataccaaaca tgattatcca tagtatacta
     4561 gctttagcag tttcaggagc tccaacccct cttgaaaatt ctgcaccaac aagcattaag
     4621 aatgcaagac ctaagaatat taatccaaga cctgtctttt taaataaact catatctcct
     4681 tgtggtctac tagcaagttt aaaccataat gctccaagaa ctggtcctaa tactatacat
     4741 acaagtgaat ttaatgagtc aaaccaagca agaggaacat caaatcctcc aacattcatg
     4801 tttacaaatg ctgcaccata atcataaact gcaagataag ttagatacca gaatacccag
     4861 aaaattacag agaagaatga tataagtata atagcccaaa ttctcttctt atccatgctt
     4921 gtaagaggag acttatcttt ttcttcaatt ttttcagcaa ctacaccttc tttaaaaggt
     4981 ctctttcctg cattcccaag gaatctccat ccatatatga accatattgc acctattaca
     5041 caaagtacag ctgctataaa gaaacattga gaaaatccaa gtacaccatt ttttgcaaat
     5101 gttttaaggt aaagaatacc aactgctata gtaccaataa aagaacctat attaacaaaa
     5161 gaatactgta ctgaaaatgc tgtatcaagt tcttcttgac tatcaaaaag ctgtccattt
     5221 accgcagata cgttaccttt aaagaatgca gtaccaattg aaactaagat tatcattgca
     5281 tttaccatag aaactgtagt tgcaatagaa ccaagatagt atcctactcc catgataagc
     5341 atacctatag gtatagtata tctagctcct atccatctat ctgatatata tcccccaatt
     5401 ataggtgcaa ggtatgtgaa tgcaacaagg ttggcttgca ttatagcagc ttgtccttta
     5461 tctattccaa gaccaccatg aactatagct gttgtaagga atatatatat taagtacttt
     5521 gaagcataat atgctgctct ttcaaatgag aaagttgttg agcagatata aaatcccata
     5581 ggaaatttac ccttcttttt tactgttgag ctcataattt taaatccttt cttaattgta
     5641 tttttatttt ttacgttttg tccaaattct ttcttttatg aaagatataa tattattatt
     5701 ttgtaaaaca atttgtaatt attattaaga taattgcgca tttatttgta gagtacatat
     5761 tctcatggaa taaaattaat acacttctta atcataaatt taaattatat aaattcacta
     5821 ttttcagata tagttataaa ttaatatatt ctcatctaat accatgaaaa aaccttcgcc
     5881 atatgcaatt cttatttttt acgtgatagt attttacaaa ctaaaatccc ccttttgtag
     5941 caacttatgt aattacattt tagtgctttt ttaaaggagt ctcgaataaa gcaacatata
     6001 ttttgttcaa ataatgtttt tatttatttt ttaaatttat atattacttc actcaaccaa
     6061 ttctcaactt aaataactaa actactatct attcacagat taggcttatt tcttacacat
     6121 tagtttataa taaacactgt gaatctaaac ataaaaactg ttattttaaa gtaattaccc
     6181 atatattcgc tttgatacta tttattgagg ttatactctt tatgtataat atattattta
     6241 taaaatacct caaaatatat tattcataaa atacctcaaa ctgaaaactt tttcacgaat
     6301 atttattttt cacctcccct tttttatttc accatgtaaa tcgtaccata aatatgcact
     6361 tatatgcaag aaaaaaatct attaatcgac agtaaataat tagaaaaatt caaattatat
     6421 ttatcaagta tattatataa aatatttcct atgaaaatat aaattaaatt aatgttcaga
     6481 attgttttta gatatgaaat cttgattttt ttattacttt acttatttgc tcaaat
//
LOCUS       DAEPIN010000135         6043 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.137, whole genome shotgun sequence.
ACCESSION   DAEPIN010000135 DAEPIN010000000
VERSION     DAEPIN010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6043
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.137"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            complement(613..2748)
                     /gene="rnr"
                     /locus_tag="KR250_003625"
     CDS             complement(613..2748)
                     /gene="rnr"
                     /locus_tag="KR250_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HBH0069504.1"
                     /translation="MIPGLKESLLGLINESAYNPLKKEELAEIFDIHSSEMPMFYNFL
                     NELEEDGYICFTKKGKIVSPNQMGYFVGKFVSHKKGFGFVESDVEYTQDLFISSDNIN
                     GAMHNDRVMAEIVVPATEEKRAEGRIVKVIKREITRIVGTFQSSKTFGFVTPDNKKFT
                     KDIYIPKKHFSGAVDNDKVVCEITVWPQEDRKPEGKIIEILGQKGERGVEIDSIIREH
                     GLPEEFPKKVLQEAEAVAVEIPQEEIKRRRDLRDLNIFTIDGDDAKDLDDAISIEVLP
                     NGNFKLGVHIADVTHYVREKNKLDKEALKRATSVYLVDKVIPMLPKTLSNGVCSLNPF
                     EDKLTLSIFMEIDHKGNVVKHEICESVINSKARMTYTEVSDILEKDDEKLKKTFEHVV
                     EDFKNAEILARILMSRREKRGAIDFNFPEAKIILNGKGEVVDIKPYERRISNKIIEEF
                     MLISNETIAEHFYWMGIPFVYRIHETPSLEKMEELSKFISTFGYIIKGDKEEVHPKAL
                     QGIIEKIKGKKEEGAISTIMLRSLKQAKYSPECSGHFGLAAKFYCHFTSPIRRYPDLQ
                     IHRIIKESLNNKISGKRQEQLTTIVDYASVQSSEKERKAELAERDVHDFYKALYMKDK
                     VGQEFEGVVSSVTSFGIFVELDNTIEGLIRLANMNDDYYIYDESSYSVLGERTKKSYK
                     IGDLVKIKVESVNVDFKEIDFEIVEKIEE"
     gene            3341..3955
                     /locus_tag="KR250_003626"
     CDS             3341..3955
                     /locus_tag="KR250_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HBH0069505.1"
                     /translation="MEEVIAKIITIENGFKEIENIAKKIVKNNDSKKCYYLSIYLYRS
                     EYYQVRELAVFILGFISVTISEALLFLKDNVSKDENWRVQEILAKSFDYYCSEVGYEK
                     ALPVIKEWLNSDSPNIKRAVTEGLRIWTNRDYFKSNPDVAISLLSSLKEDDSEYLRKS
                     VGNALRDISKKYPDLVKKELDSWDISNKKVEFVYKLASKCILAK"
     gene            3973..4335
                     /locus_tag="KR250_003627"
     CDS             3973..4335
                     /locus_tag="KR250_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0069506.1"
                     /translation="MKLRDEYTCPLELTHDMIKGKWKSIILWRLRIGPTSLSKLEKEI
                     NGINQKMLLEQLKELIEFGLVDKKTYEGYPLKVEYSLTPIRGKQILDALAIMQDIGIS
                     FMLDNGMEDILKEKGVLK"
     gene            4441..4938
                     /locus_tag="KR250_003628"
     CDS             4441..4938
                     /locus_tag="KR250_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HBH0069507.1"
                     /translation="MKVTSTGIVNGVIEDKYGKRGTQFNEGGMPTYSLPLKFEDAPKN
                     TVSFAVFLEDKDSVPVCGFAWIHWLAVNITKDELNENESVTATDFVQGTTSWHGKLGG
                     LDRLEASVYGGMAPPDRPHVYEIHVFALDTILDLEKGFYMNEFFKAIEGHVLDTFTLK
                     GSYSN"
     gene            5742..>6043
                     /locus_tag="KR250_003629"
     CDS             5742..>6043
                     /locus_tag="KR250_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH0069508.1"
                     /translation="MIAVKKAYKFRIYPNKKQQELINKTFGCCRFVYNKYLAKRIDVY
                     KNDKETFTYKQCSSDLTNLKKELNWLKEPDKFSLQNALKDLDNAYKKFFKEKAGFP"
BASE COUNT         2050 a          896 c          766 g         2331 t
ORIGIN      
        1 ttatctttca gctagctttt tctattaaat gtttctcaat tacttatttt tcaattagcc
       61 acttctatta agcatttctt aattacttat ttttcaatta gtcacttcta ttaaacattt
      121 cttaattatt tatttttcaa ttagccactt ctattaagca tttcttaatt acttattttt
      181 caattagcca cttctattaa gcatttctca attacttatt tttcaattag ccacttctat
      241 taagcatttc ttaattactt atttttcaat tagccacttc tattaagcat ttcttaatta
      301 cttatttttc aattagccac ttctattaaa catttcttaa ttatttattt ttcagttggc
      361 tttttctatt aaatgtttct taattactta tctttcagtt agctctttct attaaacatt
      421 tctcaattac ttatttttca attagctact ttcattaaat atttcttaat tatttatctt
      481 tcagttaact atttccatta gatatttctc aattacctat ttttatcatt tattttatag
      541 agtattgtgc actatatatt tatacatatc ataaatacta caacttgtta tctcttattt
      601 tagctgtttt atctactcct ctattttttc aactatctca aagtcaattt ctttaaaatc
      661 tacattaaca ctttcaacct ttatcttaac aaggtcacct atcttatatg acttcttagt
      721 tctttctcct aaaacactat aagaactctc atcataaata tagtaatcat cattcatatt
      781 tgcaagtctt atcaaccctt ctatggtatt atctaactca acaaatattc caaatgaagt
      841 tacgctagat acaacaccct caaattcttg accaacttta tccttcatat aaagagcctt
      901 atagaaatca tgtacatctc tttcagctaa ctctgccttt ctttctttct cggatgattg
      961 aacagatgca taatccacta tagtagtaag ctgttcttgt ctttttccac ttattttatt
     1021 atttaaagat tcttttatta ttctatgaat ttgaaggtca ggatatcttc ttattggtga
     1081 agtaaagtga cagtagaatt tagctgctaa tccaaaatgt ccactacatt ctggtgagta
     1141 cttagcttgt tttaaagaac gaagcattat agtactgata gctccttctt cttttttgcc
     1201 ctttattttc tctattatac cttgtaatgc ttttggatgt acttcctctt tatcaccttt
     1261 tattatatat ccaaatgtag atataaactt actcaattct tccatttttt ccaaagatgg
     1321 tgtctcatgt attctataca caaacggtat tcccatccag taaaaatgtt cggcaattgt
     1381 ctcattactt ataagcataa attcttctat tatcttatta gatattcttc tttcatatgg
     1441 ctttatatct acaacttcgc cttttccatt tagaattatc tttgcctctg ggaaattgaa
     1501 gtctatagct cctctttttt cccttcttga cataagaatc ctagctaaaa tttcagcatt
     1561 tttaaaatct tcaacaacat gttcaaaagt ctttttaagt ttttcatcat ccttttctaa
     1621 tatatcagat acttctgtat aagtcattct agcttttgaa tttattacag attcacatat
     1681 ttcatgttta accacatttc ctttatggtc tatttccata aatatagaaa gagttagctt
     1741 atcttcaaat ggatttaagc tacatactcc atttgaaaga gtttttggca acattggaat
     1801 tactttatct actaaataaa ctgatgtagc tctttttaag gcttccttgt caagtttatt
     1861 tttctctctt acataatgtg taacatcagc tatgtgaact cctagtttga agtttccatt
     1921 aggtaatact tctattgata tggcatcatc taagtctttt gcgtcatctc catctatagt
     1981 aaatatgttt aaatctctta aatctcttct tctttttatc tcttcttgag gaatctcaac
     2041 agctacagct tctgcttctt gcaatacttt ctttggaaat tcttctggaa gaccatgttc
     2101 tcttattata gaatctattt ctacgcctct ttcacctttt tgacctagta tctctataat
     2161 tttgccttct ggttttctat cctcttgtgg ccatactgta atttcacata ctaccttatc
     2221 attatctact gctccactaa aatgtttttt aggtatatat atatctttag taaatttctt
     2281 gttgtctggt gttacaaatc caaaagtttt acttgattgg aaggtaccta ctattctagt
     2341 tatttctctc tttattactt ttactattct tccttctgct ctcttttctt cagttgcagg
     2401 tactactatt tctgccataa ctctatcatt atgcatagca ccatttatat tgtcacttga
     2461 gataaataaa tcctgtgtat attcaacatc agattctaca aatccaaaac cctttttgtg
     2521 tgatacaaat tttccaacaa agtatcccat ttgattggga gatactattt ttcctttttt
     2581 agtaaagcag atataaccat cttcttctaa ttcatttaaa aaattataaa acattggcat
     2641 ttctgatgaa tgtatatcaa atatttctgc taattcttct ttttttagag gattatatgc
     2701 actttcattt attaatccta gcaaactttc tttaagtcct ggtatcattt ttccctcctg
     2761 ttttactttt cattatatta ttatttatat tgtcttttat atttcttcag acaaaacatt
     2821 ttttacttat cacataattt tctatatttt cttattatta taagttaaaa ttacttattt
     2881 atatttttga tttaatgtaa tctttgtcat actaacttca aacaaattat gatgttttat
     2941 aaattatttg taaatatcat tataattact taagttattc tttatcataa ttgcttaaat
     3001 tactctttat cacctaatta tacaccatca aataggattg gtaaaatatt catctcatat
     3061 aagtattact atataataat ttattctatt agtatactca aatcatgtaa aatttatata
     3121 actcaattaa ttttttaaat acaactttat aatataaaac ttataataaa agtaaattta
     3181 tatgttaaat gaggttgatt ttttattatt ttttatttat gctagtacta aagacttgca
     3241 aacgtttttt gacaagtctt tattttgtct aaattaaaca aactttttta caaataaata
     3301 cataaaattt aaactaatct atttattgga ggagataaaa ttggaagaag taattgcaaa
     3361 aattattact atagaaaatg gattcaaaga aattgaaaac attgctaaaa aaattgtaaa
     3421 aaataatgac tcaaaaaagt gttattatct atcaatctac ttatataggt cagaatatta
     3481 tcaagtaaga gaattggctg tgtttatact tggttttata tcagtaacta tttctgaagc
     3541 tttgttattt ttaaaagata atgtgagtaa agatgaaaac tggagagtac aagaaatact
     3601 agctaaatca tttgattatt attgcagtga agtaggatat gaaaaggcac ttcctgttat
     3661 aaaagaatgg cttaattctg atagtccaaa cataaaaagg gctgtgacag aaggtcttcg
     3721 aatttggact aatagagact attttaaatc taatcctgac gttgctatat cactactttc
     3781 tagtttaaaa gaagatgata gcgaatactt gagaaagtct gtaggtaatg ctcttagaga
     3841 tataagtaaa aaatatccag atttagtaaa aaaagaatta gattcatggg atatatcaaa
     3901 taaaaaagtt gagtttgtat ataagcttgc ttcaaaatgt atcttggcaa agtaaaatgg
     3961 aggtgaactt atttgaaact tcgtgatgag tatacttgcc cattagaact aacacatgat
     4021 atgataaaag gcaaatggaa atctattata ttatggagat tacgcatagg accaacttcc
     4081 ttatcaaaac ttgaaaagga aatcaatggt attaatcaaa agatgttact tgaacaactt
     4141 aaagaattaa ttgaatttgg tcttgtagat aaaaagacat atgaaggtta cccacttaaa
     4201 gtcgaatact cacttacacc tattcgaggg aaacaaatat tggatgcttt ggctataatg
     4261 caagatatag gaattagctt tatgttggat aatggtatgg aagatattct taaggaaaaa
     4321 ggtgttttaa aataaataga tacccacaaa aaagtaagta atttaccatt attttcaact
     4381 ggtatataat taaattatag atatattcta ttaatttatt ataataggag gtaaattatt
     4441 atgaaagtaa ctagtacagg tatagtaaat ggtgttatag aagataaata tggtaaaaga
     4501 ggaactcagt tcaatgaagg tggtatgcct acatattcac tacctctaaa gtttgaagat
     4561 gctccaaaga atacagtatc ctttgctgta tttttagaag ataaagactc tgttcctgta
     4621 tgtgggtttg catggataca ttggttagct gtaaatataa caaaagatga gttaaatgaa
     4681 aatgaaagtg taacagctac tgattttgta caagggacta ctagttggca tggtaaacta
     4741 ggaggtcttg acagattaga ggccagtgta tatggtggaa tggctcctcc agacagacct
     4801 catgtctatg aaatacatgt atttgcactt gatactatcc ttgaccttga aaaaggattc
     4861 tatatgaatg aatttttcaa agctattgaa gggcatgttt tagatacatt tacactaaaa
     4921 ggaagttatt ctaattaatt aggttgacta tagctaatca ttaattaaat gatacattta
     4981 attgatgatt agcttaattg ttattagaat attttctgca ctttatctta atatatattt
     5041 tagttgactg gtattttaat ttctattaga ttattatcaa tttatataaa ttgattaaaa
     5101 aatagctacg cacactcgtg actttagtca tgagttagta gctaaaatag tcagcatata
     5161 gggaaactta tatgtagaga taggatagaa cctatccaat actacttgaa ttgctaaaaa
     5221 cccctaaagc taaccaaact aaaacataag gatgaaataa acctaagtgt gaaagttacg
     5281 aaagtagaaa aaattggtta gatggtataa ggttaaaccc taagtacttt aaaatgggca
     5341 atcagcaggt aaactccaaa taggagaaac ttcaacgact attcctcttg agggaagtac
     5401 actacaagtt atcggtagtg gaagtgggta gaccttagca gataatgccg aaggataaga
     5461 tatagtctgt gcttatgtga aagtgtaaga agttcataag agaactgcat aggtagtagc
     5521 gaacttatgt gaacgacaac ctcaaaaatg ataaaagtta agacttacgg ttcttatata
     5581 tgttatttaa aattaaatat aaaaccttct aaataattgt tttaaataat tacatttgtt
     5641 aacattatta taggtgatta ataaattgtt caccacagca ttatataata tattaaatat
     5701 taaaatcatt aaaaagtgta tcaaaaaaca attaaagtga ggtgatagca gtgaaaaaag
     5761 cttataagtt tagaatatat cctaataaaa aacagcaaga gttaattaac aaaacttttg
     5821 gatgttgtag gtttgtatat aataaatatc ttgctaaaag aatagatgtt tataaaaacg
     5881 ataaagaaac ttttacttat aaacagtgta gttctgattt aaccaattta aaaaaagaat
     5941 taaattggct taaagaacct gataaatttt cactacaaaa cgccttaaaa gatttagata
     6001 atgcatataa aaaattcttt aaagaaaagg caggatttcc taa
//
LOCUS       DAEPIN010000136         6036 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.138, whole genome shotgun sequence.
ACCESSION   DAEPIN010000136 DAEPIN010000000
VERSION     DAEPIN010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6036
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.138"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            <1..789
                     /locus_tag="KR250_003630"
     CDS             <1..789
                     /locus_tag="KR250_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HBH0069509.1"
                     /translation="SSFLNNSKEIAAYLFDVMKLNATLESCDLAICLYSQKDEKKVAI
                     LKLDYNKSYTHSIEFKDDKFNIQMSKNEINIQETKTVKIAALVGLSGMNDEYHLRVLD
                     KDAEKEEANSKFITEFLNATKVKDDKYKTKMFKSTAENWITNALGNDIKQAEDVRSIL
                     NYTLKEKHEIDINNFVDKTIKDDKLKDSFKEHMEEKGLVEGFSIDKKWVDKKLKKRNI
                     KTDNGFEIKGNLTDFEDPMKYTVRQNQNGSIDIVIKNVTFYEEK"
     gene            871..1200
                     /locus_tag="KR250_003631"
     CDS             871..1200
                     /locus_tag="KR250_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069510.1"
                     /translation="MKIFLLTILLIIICILANYVKNRIYKKSINNLKYKYSVGEKIIY
                     HQINCYYNRMVGCEILEKCYSTKFRKRNTPLYKVKAYVGDNDTTWVIPEWRIECLATT
                     YGEFPKY"
     gene            1237..1608
                     /locus_tag="KR250_003632"
     CDS             1237..1608
                     /locus_tag="KR250_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069511.1"
                     /translation="MFNIYKVKIKTKRTLEQVRNQSVDFEYSEKGLKNTLKYYNLIDD
                     LKVIVVKFGDEYCLANYNEEDRKIIMEAHYLLEQDEYTGCYINEYERFKKDWENGNCD
                     GEACMVFSDDEIEIIEKLREG"
     gene            1611..1751
                     /locus_tag="KR250_003633"
     CDS             1611..1751
                     /locus_tag="KR250_003633"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069512.1"
                     /translation="MNKRIKMKKRLIHKKCDERCVNYDFVISNNLITCNVCRMQIQRK
                     YG"
     gene            1866..2207
                     /locus_tag="KR250_003634"
     CDS             1866..2207
                     /locus_tag="KR250_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HBH0069513.1"
                     /translation="MANIYCENYNCKNYFEDMCMLERIEINNFKECESYLEGKNELYE
                     LENGYTIHPKDLKMVKSKDYSVEVTHIPTGITVKCRSTNSILKNKNKCLEALEEELTK
                     INSHLELEDLR"
     gene            2333..2740
                     /locus_tag="KR250_003635"
     CDS             2333..2740
                     /locus_tag="KR250_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HBH0069514.1"
                     /translation="MKVFLVIDGEPVGKERPRFNLATKRTYTPQKTKDYEELIKWLYQ
                     SKVRHYFTGYIKMTLRCYYSIAKSNSKKIKEQKRNNVLRPNKKPDIDNVVKIIADSLN
                     EIAYKDDTQIVEVVASKYYSDKPRVEVILEDVI"
     gene            2842..3690
                     /locus_tag="KR250_003636"
     CDS             2842..3690
                     /locus_tag="KR250_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6C domain-containing protein"
                     /protein_id="HBH0069515.1"
                     /translation="MNENINKEITVLGTLEIEGMKFHNIEGGFGEHKKAMLVKDIAEI
                     HNRESRQINELINKNRKRFKDGKDILDLLGVGLDDTKIKELGFTQQSINSYRGLKNKG
                     LLSGIYILSERGYAKLLKILEDDIAWELYEKLVDGYFSMRKELNNPLLSASKELQAIF
                     MLDKKQEVLETKIENVNEKLENFMDDAPLFNIECECIVKEVKRVATKSLGGHGSKAYK
                     NKSLRGKVYSDIYHQIKREFGVDSYKAIKRCQLDKVLEIVNSYKLPIVFEEEIRLLNS
                     QLSIVS"
     gene            3833..4321
                     /locus_tag="KR250_003637"
     CDS             3833..4321
                     /locus_tag="KR250_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069516.1"
                     /translation="MDNNINKKELFKKVEGRLHHYKFLSAEIKNLELDIESRENEIFG
                     CKAIGYGEKVSPTYAFNSTVENEIIKKERDIARLKKLKKDKEIEKKKIENALTCLDIR
                     EEHFFKLFYNSRMKNSMVYISLEMNSDRKTCRCVRERLVYKVMDMLYPRIKENELPLF
                     KN"
     gene            4903..5580
                     /locus_tag="KR250_003638"
     CDS             4903..5580
                     /locus_tag="KR250_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HBH0069517.1"
                     /translation="MLKILQEKNVKVMWSKNGEEVWFNANDVGEELGIVNIRDTLRNI
                     DREYKKKFNESTVGDSYTRNFKDKLPNFGTTFVTEEAVYNMSFRSNKPEAKLFTKWVT
                     KTLKQIRIHGYYIATEKDQEWLDIRTEGKKVRKDFTDEIQEFVYYATSQGSNKPQMYY
                     KHFTELVRKKLGIPKGVKRDELNQSELFDIQALERIISMKLPKLIDKDMNYKEVYKKI
                     KELIEMI"
     gene            5643..>6036
                     /locus_tag="KR250_003639"
     CDS             5643..>6036
                     /locus_tag="KR250_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH0069518.1"
                     /translation="MQDVKEKVKQDYIKGMKQKEISAKYDISLNTLKSWIKRYNWASE
                     KKKGAPKNKRGAPIGNKNATGPPGNKNAEKFGFFSKYLPEETRELIQEISIKDKFDIL
                     WEQITIQYAAIIRAQKIMYVKDKEEMIKE"
BASE COUNT         2546 a          597 c         1129 g         1764 t
ORIGIN      
        1 agttcatttt taaataactc taaagagatt gcagcttatt tatttgatgt tatgaaattg
       61 aatgctacat tagaatcttg cgacttagca atttgtttat actctcaaaa agatgaaaag
      121 aaagttgcta tattaaagct tgattacaac aagtcatata ctcattcaat cgagtttaaa
      181 gatgataaat ttaatataca aatgtctaaa aatgaaatta atatacaaga gactaagaca
      241 gtaaaaattg ctgctttggt tggtttgagt ggaatgaatg acgaatatca tcttagggtt
      301 ttagacaagg atgcagaaaa ggaagaagct aattctaagt ttattacaga gtttctaaat
      361 gctactaaag tgaaagatga caagtacaag actaagatgt ttaaaagtac agccgaaaat
      421 tggataacta atgctcttgg aaatgatata aaacaggcag aagatgtaag aagtatatta
      481 aattatactt tgaaagaaaa gcatgaaatt gatataaata attttgttga taaaacaatt
      541 aaagatgata agttaaaaga tagttttaaa gaacatatgg aagaaaaagg tcttgttgaa
      601 ggatttagta tagataaaaa atgggttgat aaaaagctta aaaagagaaa tataaaaact
      661 gacaatggct ttgaaataaa aggtaactta actgattttg aggacccaat gaaatataca
      721 gtaagacaaa atcaaaatgg gtctatagat atagttatta agaatgtaac attttatgag
      781 gaaaagtagg tactcatgtg actattggct agagaaggag aagtaaataa taagaggatg
      841 taaatttaaa ctagttagga ggaataactt atgaagattt ttttattgac tatactgcta
      901 ataattattt gtatattagc aaattatgtg aaaaatcgca tatataaaaa atctataaat
      961 aatctaaaat ataaatattc tgtaggggaa aagattatat atcatcaaat aaactgttac
     1021 tataacagaa tggttggttg tgaaatttta gaaaaatgtt atagtacgaa atttagaaaa
     1081 agaaataccc cgctttataa agtaaaagca tatgtaggtg ataacgatac aacatgggtt
     1141 ataccagagt ggagaattga atgtcttgct acgacttatg gagaatttcc taaatattaa
     1201 ataataaaaa ttggctggag aaggagattg taaattatgt ttaacatcta taaagtgaaa
     1261 ataaagacta aaagaacatt ggagcaggta agaaatcaaa gcgtagactt tgagtattca
     1321 gaaaaaggat taaaaaatac tctgaaatac tataacttga ttgatgattt aaaagtaata
     1381 gtagttaaat ttggagatga atattgtcta gctaattaca atgaagaaga tagaaaaata
     1441 ataatggaag cacattatct tttagagcag gatgaatata ctggatgtta tataaatgaa
     1501 tatgaacgat ttaaaaaaga ttgggaaaat ggtaattgtg atggggaagc ctgtatggta
     1561 ttttcagatg atgaaattga gataattgag aagctaaggg agggttaaat atgaataaaa
     1621 gaattaaaat gaaaaaaaga ttaattcata aaaagtgtga tgaaagatgt gtcaactatg
     1681 actttgtaat tagcaataac cttataactt gtaatgtgtg taggatgcaa atacaaagaa
     1741 aatatggata aagtatgtga agagaactat aagaaattaa gaagtaaata gaataaaata
     1801 gtcaaggtaa gtttgtgaat gaaactagaa tgttataggc ttgacttata aaaggagtgc
     1861 gttaaatggc taatatatat tgtgaaaatt ataattgtaa aaactacttt gaagatatgt
     1921 gtatgcttga aagaattgaa attaataact tcaaagaatg cgaaagctat cttgaaggta
     1981 aaaatgagct atatgaatta gaaaacggat atactataca tcctaaagat ttgaaaatgg
     2041 tgaaaagtaa agattattct gttgaagtta ctcatattcc aactggtatt acagtaaaat
     2101 gccgttctac aaatagtatt ttaaaaaata aaaataagtg tttggaagct ctagaagaag
     2161 aactaacaaa aataaactct cacttagagc tagaagattt acgctaaata ggaagtgagc
     2221 ttatgaaacg aagaagatgc agttggtgtg gtaagttatt ttatcttgaa gaaaaatcta
     2281 aggatgttta ttgttgtaaa gaatgtagga agaaggctaa gaaggtgaaa aaatgaaagt
     2341 ttttcttgta atagatggag aaccagttgg taaagaaaga cctagattta acttggctac
     2401 taaaaggacc tatacaccac agaaaactaa ggactatgaa gaattaataa aatggttata
     2461 tcaatctaaa gttagacatt attttactgg atacataaaa atgactttaa gatgttatta
     2521 ctctatagca aaaagtaaca gtaaaaagat taaagagcag aaaagaaata atgtgttaag
     2581 acccaataaa aaaccagata ttgataatgt ggtcaagatt atagctgatt cactcaatga
     2641 gatagcttat aaagatgata cgcagattgt tgaggttgta gctagtaaat attacagtga
     2701 taaaccaaga gttgaggtta tattagaaga tgttatttaa ccaacggaaa aatccgttcg
     2761 ttagattagg tactcagttt ttcataaaaa tgtgaaaaag ctgaatagag aatatatcaa
     2821 atggtaaagg agagataaat tatgaatgaa aatataaata aagaaataac agtacttgga
     2881 actttagaaa tcgagggaat gaaatttcat aacattgagg gtggatttgg agaacataag
     2941 aaagcaatgc tagtaaaaga tatagctgag atacataata gagaatctag acagattaat
     3001 gagcttataa ataagaatag aaaaagattt aaagatggaa aagatatatt agatttgtta
     3061 ggtgtcggtt tggacgatac caaaataaaa gaattaggat ttactcaaca atcaattaat
     3121 tcttatagag ggttaaaaaa caaagggtta ttatctggga tttatatatt atctgaaaga
     3181 ggttatgcaa aattattaaa aatattagaa gatgatatag cttgggaatt atatgagaag
     3241 ttagttgatg gatatttctc tatgagaaaa gaactaaata atcctctttt aagtgcatca
     3301 aaggagttac aagctatatt tatgctagat aagaaacaag aagtcttaga aactaaaata
     3361 gagaatgtta atgagaagtt agagaacttt atggatgatg caccattatt caatatcgag
     3421 tgtgagtgta ttgttaaaga ggttaagaga gtagcaacaa aatcacttgg tggacatgga
     3481 agtaaggctt ataaaaataa atctttaaga ggtaaagttt atagtgatat ataccatcag
     3541 attaaacgag aatttggagt agatagttat aaggctataa agcgttgtca attagataaa
     3601 gtattagaga ttgtaaatag ttataagtta cctatagtgt ttgaagaaga aataagactt
     3661 ttgaatagtc aattatcaat tgtgagttaa gacaggaata gatttttctc ctttaaaggg
     3721 gaaaacctgc aatagttaat taaaagagaa aaggagtgct ttcacactcc acttgtcaaa
     3781 aatataaagc ttttatccaa gattattata acataaacag gagtgtgaaa gtatggataa
     3841 taatattaat aaaaaagaac tatttaaaaa agtagaaggt agactacatc attataaatt
     3901 tttaagtgca gaaattaaaa atcttgaatt agatatagaa agtagagaaa atgaaatatt
     3961 tggttgtaag gctatcgggt atggtgaaaa agtaagtcca acatatgctt ttaattcaac
     4021 tgttgagaat gagattataa aaaaagaaag agatattgct agattaaaaa aactgaaaaa
     4081 agataaagaa atagaaaaga agaaaataga aaatgcactt acatgcttag atataagaga
     4141 agaacatttt tttaaactgt tttataatag cagaatgaaa aatagtatgg tttatatatc
     4201 cttagaaatg aactcagata ggaaaacatg tagatgtgta agggaaagat tagtgtataa
     4261 agttatggat atgctttatc caagaattaa ggaaaatgaa ctcccattat ttaaaaatta
     4321 gaaaattccc cagtttttcc ccagaaattc ccactttatt ccctactttc tccccgtttt
     4381 gattaaaaaa gcatgagata atagtattgt ggaaataaag atttccctct caaaacttaa
     4441 tatttgacta gggttaaggg attgccctag tcactatgaa cagactaggc agggcatgag
     4501 gatgctgtta gttcaattct aactatgttc aatctttagt ttttctattt caattaatct
     4561 acggatacac taaaaaatag tatatgaatt gagattaaaa tctcatacaa ttttgtatct
     4621 taattcaaag actaaaaacc gagtggggct tggtaacctc actcaccatg caagtactgg
     4681 tttaatctag gttcgattcc tggaacttgc tccctttaat aatatgtatc ccccattaaa
     4741 aaggcttaga ttaacttcta ggtctttttt aatacaaaaa tttattaaaa atgcacggca
     4801 tgcactattc agatatgcat gtcatgcatg tctaaagtcg aatatttaat attttcgatg
     4861 gctcaaattg aggggtcgaa aataaaaaca ggaggtagta gtatgttgaa aattttacaa
     4921 gagaaaaatg taaaagtaat gtggtccaaa aatggagaag aagtttggtt taatgcaaat
     4981 gacgtaggag aggaactagg catagtaaat attcgtgata cattaagaaa tatagataga
     5041 gaatataaaa agaaatttaa tgagtctact gtcggagatt cctacactag aaactttaaa
     5101 gataaattgc ctaacttcgg tactactttt gttacagaag aagctgtgta caatatgtca
     5161 tttagaagta ataaaccaga agcaaagtta tttacaaaat gggttacaaa aacacttaaa
     5221 caaattagaa tacatggtta ttatattgct acagaaaaag accaggaatg gctggatata
     5281 aggacagaag gcaaaaaagt aagaaaagat tttacagatg aaatacaaga gtttgtatat
     5341 tatgctacta gtcaaggtag caataaacct cagatgtatt ataaacattt tactgaactt
     5401 gtaagaaaaa aattaggtat tccaaaaggt gtgaaaagag atgagttaaa tcaaagcgaa
     5461 ctatttgata tacaagcact tgaaagaatt atatctatga aattacctaa gttaatagat
     5521 aaagatatga attataaaga ggtatataaa aagattaagg aattaataga aatgatttaa
     5581 gggactgtct tgatggaggg tcttttttaa ttcccaaaac gacaaacaaa cgaggtggtg
     5641 atgtgcaaga tgtcaaagaa aaggtaaaac aagattacat aaaaggtatg aaacaaaagg
     5701 aaatatcagc aaagtatgac attagtttaa acactttaaa gtcatggata aaaagataca
     5761 attgggctag tgagaaaaag aagggtgcac ctaaaaataa aagaggtgca cccataggta
     5821 ataaaaatgc cactggtcct cctggaaata aaaatgctga aaagtttggt ttcttctcaa
     5881 aatatctacc tgaagaaact agggaattaa tacaagaaat atctataaaa gataaatttg
     5941 atattctttg ggaacagata acaattcaat atgcagcaat aataagagca caaaagataa
     6001 tgtatgttaa agacaaggaa gaaatgatta aggagt
//
LOCUS       DAEPIN010000137         5759 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.139, whole genome shotgun sequence.
ACCESSION   DAEPIN010000137 DAEPIN010000000
VERSION     DAEPIN010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5759
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.139"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            complement(101..880)
                     /locus_tag="KR250_003640"
     CDS             complement(101..880)
                     /locus_tag="KR250_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBH0069519.1"
                     /translation="MKKIIWGICICLLSIVGVYTYNHNTHNQDSSKEAYSLNDNIKDD
                     YEDVIIKKGNEDEKILALTFDDGPDEDFTPQVLDILKKNDVKATFFVVGEKVEYNKEL
                     LKRQYDEGHEIGNHTFTHINVAKNSYSSVEKEITDTQNIIKSVIGVEPKIFRPPYRAM
                     SKSVCDIIVSKGMNIILWSNLDPRDWSNPGVDAIINTILTKVQNGNIILLHDYNTKRN
                     DKSQTIQALEVVIPKLKERGYKFVTVSELIQHLDKNKQVQK"
     gene            complement(935..1417)
                     /locus_tag="KR250_003641"
     CDS             complement(935..1417)
                     /locus_tag="KR250_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HBH0069520.1"
                     /translation="MDTKKYKDKLLKERENLTNLVEDMKDNTLFGDTTKHTSEKYSSG
                     ELSSYDNHIGDMGTDLYMQNMQNSLINHEEGRLYQIDLALSKIENGTYGICDLCHNQI
                     DLERLDILPDTSLCNDCAKKENNLLGDAMQNPDVEDSNFYSEDLTNLTDLNKNGILRD
                     "
     gene            complement(1683..3104)
                     /locus_tag="KR250_003642"
     CDS             complement(1683..3104)
                     /locus_tag="KR250_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HBH0069521.1"
                     /translation="MVSFLGSIVVLIVGYFVYGTFVEKVFGTNDKNQTPAIACRDGVD
                     YVPMKWKRIFLIQFLNIAGLGPIFGAIQGALFGPSAFLWIVFGTIFAGGVHDFASGYL
                     SMKNKGTSASELVGLYLGNGAKIAMRIFSVILLVLVGVVFVTGPAGLLKTLTGVNAQI
                     WVGVIILYYIMATVLPIDKLIGKIYPLFGAALLIMAVGVAGGLIIKGYNIPNINLQNM
                     NPNGTPLFPYLFITIACGAISGFHATQSPLMARCVEKESEARPVFYGSMVAEGIIALV
                     WAAAAMSYFHGIPQLNVIFNDGGAATVVNTVSVGLMGPIGGALAILGVVACPITSGDT
                     AFRSARLTIADAMNYNQDAVKNRFLIALPLFAVGVALTFIDFNIIWRYFSWANQTLAM
                     IMLWTGSAYLVKANKNHYITTLPALFMTVVTFSYIMQAKEGFRLPVNISNGIGVIVAI
                     ILGLLFFKKAKHIKEQNNHKLAS"
     gene            complement(3250..4020)
                     /locus_tag="KR250_003643"
     CDS             complement(3250..4020)
                     /locus_tag="KR250_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0069522.1"
                     /translation="MRAIIVEDEFPARKELRYFIENKSGIEVVSEFTNGIEVLDFIQE
                     NKIDVIFLDINIPHLDGMLLAKTLNQFKSRPKIVFITAYESYAVDAFSLDVFDYILKP
                     YSEERIISMLNKLEKSEMSDIELFNVNSNLYKYKKEAVNQEIEEITHKISLWKGDKLV
                     VIDIDDIYYCEANERQTFIYTEKEKFILKEGISEVENLINDKTFFRTHRSYIVNLTKV
                     KEIIPWFNNTYILKLKNSDYEVTVSRSKVKEFRLLMHI"
     gene            complement(4025..5227)
                     /locus_tag="KR250_003644"
     CDS             complement(4025..5227)
                     /locus_tag="KR250_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBH0069523.1"
                     /translation="MKTSILIALIEKTSLIVVLFLLITKLKIFKQIFQKEEYSFNDLV
                     CIALVFTFLAIFGTYSGINYMGSIVNTRIISIVSGGILFGPMVGITAGVFSGIHRYFM
                     DIGGITSVPCLLSSILAGVLSGFLYKRIPKQHRVMYGILVGMISESFTILLIYLISYP
                     HSLAIQIIGGIYLPLIVGQIGIGFVISIVEGIEKDKKDIEARNKAEIKALQRQINPHF
                     LFNSLNTIASFIRFSPDKARELIINLSTYLRYNLEYSDNLIDINKEIEQVKSFVEIEK
                     ARFGELLTVSYDIEDVNIKIPSLIIQPLVENAIIHGILESGRAGVVKISIKKLPYSLE
                     NTVRISIEDNGIGISEEIINNVYQDNMPENKIGLYNVHLRLKLMYGRGLNIRRIDNGT
                     LIVFYVKE"
BASE COUNT         1896 a          997 c          566 g         2300 t
ORIGIN      
        1 taccatacta atcaatattt tttgtgtatt ttatagatta aagattaatg atgtctgtaa
       61 tttaaactta caatcaccac taatcttcaa acaaatattt ttacttttgc acttgcttat
      121 ttttatctaa atgttgtata agttcagata ctgttacgaa tttatatcct ctctctttta
      181 atttaggaat tactacttct aaagcttgta tagtttgtga tttatcattt cttttagtgt
      241 tataatcatg taaaagtatt atattaccat tttggacctt tgtaaggata gtatttataa
      301 ttgcatctac tccagggtta gaccaatctc tagggtctaa gtttgaccaa agaattatat
      361 tcatgccttt agaaactata atatcacaga cacttttact catggctcta tatggaggtc
      421 taaatatctt tggttctact cctattacac tttttataat attttgcgta tctgttattt
      481 ctttttcaac actactataa ctattttttg caacatttat atgtgtaaat gtatgattcc
      541 ctatttcatg cccctcatca tattgtcttt ttaataattc tttgttatat tctacttttt
      601 ctccaactac aaaaaaagtt gcttttacat catttttctt taatatatca agtacttgtg
      661 gtgtaaaatc ctcatctgga ccatcatcaa aagttaatgc taaaattttt tcatcctcat
      721 ttcctttttt tattattaca tcttcataat cgtcttttat attgtcattt aaactatatg
      781 cttcttttga actatcttga ttatgtgtat tatgattata tgtatataca cctactatag
      841 ataataaaca tatacatata ccccatatta ttttcttcat gtcattacct cttttctaat
      901 tcattctagc aatatatttt ataatgtcag ttttttaatc tctcaatatt ccatttttat
      961 ttaaatcagt taaatttgtt aggtcttctg aataaaagtt agaatcttct acatcagggt
     1021 tttgcattgc atcacctaat aaattatttt ctttctttgc acaatcatta cataaacttg
     1081 tatctggcaa tatatccaat ctttctaaat ctatttgatt atgacataaa tcacaaatcc
     1141 catatgttcc attctctatt ttagataatg ctaagtctat ttgatacaat cttccctctt
     1201 catgatttat tagactgttt tgcatatttt gcatatataa atctgttccc atgtctccta
     1261 tatgattatc ataactagat aattctcctg atgaatactt ttcacttgta tgtttagttg
     1321 tatctccaaa taaagtatta tctttcatat cctctactaa attagttaaa ttttctcttt
     1381 ctttcaataa tttatcttta tattttttag tatccatttt taacacctct aattttaata
     1441 tttacatcat aaaaatagta tgtggaaaag taaattaaac atacaataaa aataagattt
     1501 gattcgaaaa atggaatgtc tcaaaatgaa atttttagtt catttaaccc tcttttttat
     1561 atgaaatcaa atatattttt atcctttcag caataaaaaa gagacttatc tctattttga
     1621 gatagtctct tctttgaaca taatttaaat acaatttttt aatttttata gttattattt
     1681 tactaacttg ccaatttatg attattttgt tctttaatat gttttgcttt tttaaagaat
     1741 aaaagaccaa gaattattgc tacaataaca cctattccat tagatatatt aactggtagt
     1801 ctaaatcctt cttttgcttg cattatataa ctaaatgtta ctacagtcat aaaaagtgct
     1861 ggtaatgtag ttatataatg gtttttattt gctttgacta aatatgcaga accagtccac
     1921 aacattatca ttgcaagtgt ttgatttgcc caagagaaat atctccaaat tatattaaaa
     1981 tctataaatg taagagcaac accaactgcg aataatggta aagctattaa aaatctattt
     2041 ttgactgcat cttgattata attcattgca tctgctattg tcagcctagc acttctaaat
     2101 gctgtatctc cagaagttat aggacatgcc acaactccaa gtatagctaa agctcctcca
     2161 ataggcccca ttaatccaac agatactgta tttactactg ttgctgctcc accatcatta
     2221 aatatcacat taagttgagg tatgccatgg aaatatgaca tggctgctgc tgcccaaact
     2281 aaagctatta taccttctgc aaccattgac ccataaaata ctggtcttgc ctcactctct
     2341 ttctcaacac atctcgccat aagtggtgat tgagttgcat gaaaacctga tattgctcca
     2401 catgctattg ttataaataa gtatgggaat aaaggtgttc catttggatt catattttgt
     2461 aaatttatat ttggtatgtt ataccccttt attattaatc ctccagctac acctactgcc
     2521 ataattaata atgctgctcc aaataaagga tatattttac caatcaattt atctattggt
     2581 aaaacagtag ccataatata ataaagtatt atcactccaa cccatatttg tgcgtttaca
     2641 cctgtcaatg tctttaataa tcctgctgga cctgttacaa atacaactcc aaccaatact
     2701 aaaagaataa cactaaatat tctcattgct atttttgcac catttcctag ataaaggcct
     2761 accaactctg atgcagatgt acctttgttt ttcattgata gatatcctga agcaaaatcg
     2821 tgtactccac cagcaaagat tgttccaaac acaatccata aaaatgctga tggtccaaat
     2881 aatgctcctt gtatagctcc aaatatcgga cctaaccctg ctatatttag aaactgaatt
     2941 aaaaagattc tcttccattt cattggcaca taatctactc catccctaca agctatagct
     3001 ggagtttgat ttttgtcatt ggttccaaaa accttttcta caaaagtacc atagacaaaa
     3061 taacctacta taagtactac tattgaacct aaaaagctta ccataaattc atccccttag
     3121 tttttatata attgctttat aaatttttat gagtttaaga aaactatttc cagttcaaat
     3181 agtaatactt ttttatgcta atataaatat taattacatt aagtgcatat ttaagtacat
     3241 gaaatgactt tatatatgca ttaacagcct aaattctttt actttgcttc tacttacagt
     3301 tacttcataa tcactatttt ttaattttaa tatgtatgta ttattaaacc atggtataat
     3361 ttcttttact ttggttaagt ttacgatata agacctatgt gttctaaaaa atgttttatc
     3421 atttataaga ttttcgactt ctgatatgcc ttcttttaat ataaactttt ctttctcagt
     3481 atatataaat gtctgccttt cattagcttc acagtaatat atatcatcaa tatctattac
     3541 aactaattta tcacctttcc aaaggctaat tttatgtgtt atttcttcta tctcctgatt
     3601 tactgcttcc ttcttatatt tgtataaatt actatttaca ttaaatagtt caatatcact
     3661 catctcactt ttttctaatt tattcagcat agaaataatt ctctcttcag aatatggttt
     3721 caatatataa tcaaatacat ccaaagaaaa agcatctact gcataacttt catatgctgt
     3781 tataaaaaca attttaggtc ttgatttaaa ctgatttaat gtttttgcaa gtagcattcc
     3841 atcaagatga ggtatattta tatctaaaaa aattacatct attttatttt cctgaataaa
     3901 gtctaacacc tctataccat tggtaaattc acttactacc tcgattccac ttttattttc
     3961 aataaaatat ctcaattcct ttcttgctgg aaattcatct tccactatta tagctctcat
     4021 acttctactc ctttacataa aatactatca aagtcccatt atctattctt cttatattaa
     4081 gccctcttcc atacatcagt ttaagcctta aatgtacatt ataaagccca atcttatttt
     4141 ctggcatatt atcttgatat acattattta taatttcctc acttatacct ataccattat
     4201 cctctattga tattctcaca gtattttcta atgaatacgg taactttttt atggatattt
     4261 tcactacacc agctctacca ctttctaaaa taccatgtat tattgcattt tccactaatg
     4321 gttgtataat caaacttgga atctttatat ttacatcttc tatatcataa gatacagtta
     4381 gtaattctcc aaacctagct ttttctatct ctacaaaaga tttaacttgt tcaatctctt
     4441 tatttatatc aattaagtta tcactatatt caagattata tcttagataa gttgataaat
     4501 ttattattaa ctctctagcc ttatctggac taaatcttat aaatgaagct attgtgttta
     4561 gggaattaaa caaaaaatgt ggattaattt gtctttgaag tgcttttatt tctgctttat
     4621 ttctcgcttc tatatctttt ttatctttct caatcccctc tactatagat ataacaaatc
     4681 ctattccaat ctgcccaact attaaaggta aatatattcc accgattatt tgaattgcta
     4741 aagaatgtgg atatgatatt aagtatataa gtaatatagt aaaactttca gaaatcatgc
     4801 ctaccaatat tccatacatt actctatgtt gttttggtat cctcttatat agaaatcctg
     4861 ataatacacc tgctagtata cttgataaaa gacatggaac agatgttatc cccccaatat
     4921 ccataaaata tctatgaatc cctgaaaaaa ctcctgctgt aatacctacc atcggaccaa
     4981 ataaaatgcc acctgatact atagatatta ttctagtgtt tactattgac cccatataat
     5041 ttataccact ataagttcca aatatggcta aaaaagtaaa aactaatgct atacagacca
     5101 agtcattaaa actatattct tccttttgaa atatctgctt aaatattttt aactttgtta
     5161 ttagaagaaa caatactaca ataaggcttg tcttttctat taatgcgatt aaaattgatg
     5221 ttttcataat ctccctttaa aaaataaatt tttacacact ctaaatttat tatggttgtt
     5281 ataaattaca tatttgaagt aatcctatca caaaactttc cacatttaat cttttaatta
     5341 aataagaaat agttgtactt ctattaacaa ctaatactag ctagcataag cttgtaataa
     5401 cttgtagtta ttattaactt tacaattcta gtatttaatt ttatacttta gaaatctctt
     5461 ttcttagaaa taatactttc gaatatcata taatacaata ttatgtagat tcttactcat
     5521 acaacatatt tctttaaaaa ttttatacta ttctttgttc aaatatctca tttgatttaa
     5581 ttgtgttaaa cacacattat catctctctg ttatatattt attatatagc cgtatatcga
     5641 taaagtaaat aaatcccata tttaaaataa tatgtttgac aataaattat tataatgtat
     5701 atttaccata ataatttgtc aaccttgaaa gttacttcta taattattgt taaataatt
//
LOCUS       DAEPIN010000138         5746 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.140, whole genome shotgun sequence.
ACCESSION   DAEPIN010000138 DAEPIN010000000
VERSION     DAEPIN010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5746
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.140"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            <1..800
                     /locus_tag="KR250_003645"
     CDS             <1..800
                     /locus_tag="KR250_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069524.1"
                     /translation="LLTSEITHFKSERMDLKVEEVKNTIKQISKSIEAIEKFDKISKD
                     LNDFNDEFISGIEALKSLLEGSQSSIKTFDQSVRKLDKQFNILNIKFVKLFDKYDNQD
                     NINKEILFDIKESSKNIYNATESQFKIRDYIKNINAGFALYERSAQDLLTKLMTHENK
                     ISQNQKILLDEKFTLDDSIKNLSSIIENFSNDLQVKLDMMFENSLDIQDKLDVMFNNS
                     FMNDEVPLDSEELFKDDVNNVFNPFSEEIYEIEDKEIKVIGEDELNE"
     gene            793..1425
                     /locus_tag="KR250_003646"
     CDS             793..1425
                     /locus_tag="KR250_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HBH0069525.1"
                     /translation="MNNKYRRTVNREFEKSSFWPTFTDLLSTVLMVVILILFSSESIS
                     GSVEQDLAKNVNASVEETFKKSGIPVKVDKSNGQVTFGEKTMFDVDSDVLKPEAKEML
                     KMFVPKYIETIYKDYGDYISKIVIEGHTDDVGSYIYNLDLSQRRAYSVAKFIVGDEIG
                     DYKYKDKVTKHIIAIGRSKAELIKNGDNSVNRDASRRVELKYEININQNK"
     gene            1754..2911
                     /locus_tag="KR250_003647"
     CDS             1754..2911
                     /locus_tag="KR250_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBH0069526.1"
                     /translation="MIEISIKNKKNSKIKTLILLLIVSISIYLTGCTTLDNGNNQSNN
                     NDSLSSSISSDKNVKIHFIDTGNSDAILIQDGKTFILIDGGDNDDEGLMVDYLNNQGV
                     KDIKYLIATHSHADHLGGLDSVVKNFNIENVFVSNGSAETKSYRDFINALANKGLSPS
                     VPLENNKFYLEDSYFEVLNTNGGDTTNEQSLVLVYTNGNDKVLFTGDAEEGTEKEILP
                     KLEKVDLLKVAHHGSRSSSSQEFLDKVNPEYAVLLVGKGNSYGHPHQETMNKLKKMGV
                     KVHRSDECSDIIFESTGDGVFTSCKDGSYNKGVREDGNSGKSNSNTSKNDSFKNKQNT
                     SNSEEIVYFTPKGKSYHSTKNCSGLSRSKKILSGTIAESKKNDPCDICYGK"
     gene            complement(3058..3693)
                     /gene="lexA"
                     /locus_tag="KR250_003648"
     CDS             complement(3058..3693)
                     /gene="lexA"
                     /locus_tag="KR250_003648"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HBH0069527.1"
                     /translation="MYLDLTEKQVLILEFIKSQIILKGYPPAVREICTAVGLRSTSTV
                     HSHLNKLEKLGYIRKDPTKPRAIEVLERSKVNDVSGANQEIIELPLVGQITAGEPILA
                     QQNIEEYIPFPASLVKGSNNFVLRVKGESMINAGILDEDYVVVDKKNTALNSQIVVAL
                     INGESATVKRFFKEGNLIRLQPENDFMEPIMLNDSEVEIVGIVTGVFRVIK"
     gene            complement(3797..4924)
                     /locus_tag="KR250_003649"
     CDS             complement(3797..4924)
                     /locus_tag="KR250_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBH0069528.1"
                     /translation="MNNDKKIIKVHNKSDKPDNIVIKENELIEKCLLLENKLPVFMKD
                     YFIYLKGSVAVSTRLAYLEDINFFCSYLIETKELTNADCVKDITEDDFNTIKSRDINL
                     FLGDYCSRYYKNTEKNTLIFENNNRALARKKSSISTLFKFLYRNSQIDNNITDGFNPI
                     KLPKPQPDAIKRLEIDEVAKMLESVETGEGLTEKEKVYWRKTKLRDKAILALFVTYGL
                     RLNELRELNISSFNFSRGEFKIYRKRGKEVLMPINHTCEHVIKDYLQNERTRDELLND
                     EEKDALFLSLQNKRITAKAIRTLVKKYTSIPLDTTRDNGYSPHKLRATAATSLIQTGF
                     SIYDVQNLLDHDNVTTTQLYAAHKKNVKRDIVKNFEWIEDD"
     gene            complement(5245..>5746)
                     /locus_tag="KR250_003650"
     CDS             complement(5245..>5746)
                     /locus_tag="KR250_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069529.1"
                     /translation="APIIGGTIMIILIFKILLPDSYMKVTQNIDLKLYVSMINNLNIN
                     GFIHIILGDISNFIRIMLLTPSVYSLKYFIFMFLMYSISTHMSLSSADLKNSLNGLSF
                     IVIVIFVISSITYLFGFDNLNISSLIVKYNIFISFFMAIGFIFSIVTLIISYILSLVS
                     PFKNFS"
BASE COUNT         2164 a          719 c          805 g         2058 t
ORIGIN      
        1 atttactaac atctgaaata actcatttta agtcagaaag aatggattta aaagtagaag
       61 aggtaaaaaa tacaataaaa caaataagta agtctataga agctattgaa aaatttgata
      121 aaatatctaa agatttaaat gactttaatg atgaatttat cagtggtata gaagctttaa
      181 aaagcctttt agaaggttct caaagttcta ttaagacatt tgaccagagc gtaagaaaat
      241 tagataaaca gtttaatatt ttaaatataa aatttgtaaa gctttttgat aaatatgata
      301 atcaagataa tataaataaa gagattttat ttgatataaa agaatcttca aaaaatatat
      361 acaatgccac tgaaagccaa ttcaaaatta gagattatat aaaaaatata aatgcaggat
      421 ttgctctata tgaaagaagt gctcaagatt tattaactaa attaatgacc catgaaaata
      481 aaatctctca aaatcaaaaa atccttttag atgaaaagtt taccttagat gactcaataa
      541 aaaatctctc aagtattatt gaaaactttt ctaatgacct acaagttaaa ctggatatga
      601 tgtttgaaaa ttctttagat attcaagata agttagatgt tatgtttaat aactctttta
      661 tgaatgatga agttcctctt gatagtgaag aattatttaa ggatgatgtt aataatgtat
      721 ttaatccttt tagtgaagaa atttatgaga tagaggataa agaaataaag gtcataggtg
      781 aggatgagtt aaatgaataa taaatataga agaactgtta acagagaatt tgaaaagagt
      841 agcttttggc caacatttac agatttatta tctactgtat tgatggttgt aattcttatc
      901 ttatttagct cagaaagtat ttcaggttct gttgaacaag atttagctaa gaatgtcaat
      961 gcttctgttg aagaaacttt taaaaaaagt ggaatacctg tgaaggtaga taaatctaat
     1021 gggcaagtaa cttttggtga aaaaaccatg tttgatgtag atagtgatgt tttaaaacca
     1081 gaggctaaag aaatgctaaa aatgtttgtt ccaaagtaca tagagactat ttataaagat
     1141 tatggtgact atatatccaa aatagttata gaaggtcata cagatgatgt gggaagttat
     1201 atatacaact tagatttatc gcaaagaaga gcatatagcg ttgctaaatt tatagtggga
     1261 gatgaaatag gtgactataa atacaaagat aaagtgacta agcatattat tgcaatagga
     1321 aggtcaaaag ctgaactcat caaaaatggt gacaatagcg tgaatagaga tgcatctaga
     1381 agagtagaac taaaatacga aataaatatt aatcaaaata aataattaaa tttttataaa
     1441 aaaaaagaag gcagttggtg tgagctttaa gccttcttta aggaaaaagt ttatttattt
     1501 ttgttgatac ttaacatatt atagttttta gaagcagttt gcaatatatt aaacgtaaat
     1561 ggtcgtttaa aatgcataac tggatactac tgatgtaaaa tatttcattt tatataatat
     1621 aaaaattata cagtatcaat tttctatatt attgtactat aatctattat ttaaaatatt
     1681 atgtaaaaaa atatcataaa aacgcatatc atgttataat tactagagta ttatgaatat
     1741 ggaggcgtag tttgtgatag aaataagtat aaaaaataaa aaaaattcta aaattaaaac
     1801 tttgatactt ttattgatag tatctatatc aatttattta actggttgta ctacattaga
     1861 taatggaaat aatcaatcaa ataataatga ttctttgtct agtagtatat caagtgataa
     1921 gaatgtaaaa attcatttta ttgacactgg aaatagtgat gcaatattga ttcaagatgg
     1981 taaaactttt atattgatag atgggggaga taatgatgat gagggattaa tggtagatta
     2041 cctaaacaat caaggtgtaa aagatattaa atatcttata gctacccatt ctcatgcaga
     2101 ccatttaggt ggacttgatt ctgttgtcaa aaactttaat atagaaaatg tttttgtatc
     2161 gaatggttca gcagaaacaa agagttatag ggactttata aatgctttag caaataaggg
     2221 tttatcccca agtgtaccgc ttgaaaataa taagttctat ttagaagatt cttattttga
     2281 agttctaaat acaaatgggg gagatactac taatgaacaa tctctagtcc ttgtatatac
     2341 taatgggaat gataaagttt tatttacagg tgatgctgaa gaaggtaccg aaaaagaaat
     2401 actccctaag cttgagaaag tagacctttt aaaagtagct catcatggtt ctcgtagtag
     2461 ttcatctcaa gagtttttag ataaagtaaa tcctgaatat gcggttcttt tagtaggcaa
     2521 gggtaattca tatggtcatc cacatcagga gacaatgaat aaattaaaaa aaatgggagt
     2581 aaaagtacat agaagtgatg agtgttcaga tataatattt gaatcaacag gtgatggagt
     2641 atttacaagt tgtaaagatg gaagttataa taaaggcgtt agagaagatg gaaactctgg
     2701 aaaatcaaac agtaatacaa gtaaaaatga ttcttttaaa aataaacaaa atacatccaa
     2761 ttcagaagaa atagtgtatt ttactccaaa aggtaagagt tatcactcta caaaaaattg
     2821 tagtgggctt tcaagaagta aaaaaatatt atcaggcaca attgcagaaa gtaagaaaaa
     2881 tgacccatgt gatatatgtt atggaaaata acaaaaaaac aaccaattta tctataaagc
     2941 ttctgaactt taaatcattt aaaaaataaa ataaatgcat aaaaaaataa gctataatca
     3001 atagataaca cattttatct atacgaatag cttatttttt taagtatttt ttaatagcta
     3061 tttaataact ctaaatacac cagttacaat acctacaatt tcaacttctg agtcattgag
     3121 cataattggt tccataaaat cattttctgg ctgaagtctt attaaatttc cttctttaaa
     3181 aaacctctta actgtagcag attcaccatt tataagtgca actactatct gagaatttaa
     3241 ggctgtattt tttttgtcta ctacaacgta atcttcatct aaaattcctg cattaatcat
     3301 actttcgcct tttactctta atacaaaatt gttactgccc tttactaaac tggcagggaa
     3361 tggaatgtat tcctctatgt tttgttgagc taaaatgggt tctccagctg ttatttgacc
     3421 tactaaagga agctctatta tttcttgatt agctccagaa acatcattta ctttactacg
     3481 ttctaaaact tcaatagctc ttggtttagt agggtccttt cttatgtatc ccagtttttc
     3541 aagtttattt agatgagagt gcacagttga agtagatctt aaccctacag cagtacatat
     3601 ttctcttaca gcaggtggat aaccttttaa tatgatttga gactttataa attccaatat
     3661 caagacttgc ttttcagtta gatctaaata cataacatca ctcatttcca aacatatttt
     3721 gtttttattt ccatacactt tatattttta attataacat aatccattta tatctgcaaa
     3781 catttgttcc tgatttttaa tcgtcctcaa tccattcaaa attcttgact atatcacgtt
     3841 ttacattttt cttatgagct gcataaagct gagtagttgt gacattatca tggtcaagta
     3901 agttttgaac atcatatata gaaaatcctg tttggattag agaagttgca gccgttgctc
     3961 ttaatttatg gggactatat ccattgtctc tagtagtatc aagtggaata gatgtatatt
     4021 tttttaccag tgttcttata gcctttgctg taattctctt attttgaaga gacaaaaata
     4081 aagcatcttt ttcttcatca tttaatagtt catctcttgt tctttcgttt tgcaaataat
     4141 cttttattac atgttcacaa gtatgattta taggcattaa aacttctttc ccacgttttc
     4201 tatatatttt aaattcacct cttgaaaaat taaaagagga gatattgagt tctctaagct
     4261 catttaatct aagcccgtat gtaacaaata atgctagtat agctttgtca cgtaatttag
     4321 tttttctcca atataccttt tctttctctg taagcccttc acctgtttct actgattcaa
     4381 gcatcttagc aacttcatct atctcaaggc gcttaatagc atctggctga ggtttaggaa
     4441 gttttattgg attgaatcca tcagttatat tattatctat ctgagagttt ctgtataaga
     4501 atttaaatag agtagagatg gatgattttt ttcttgctaa agctctattg ttattttcaa
     4561 aaatcaatgt gtttttttct gtatttttgt aatatctact gcaataatca cctaagaata
     4621 aatttatatc tcttgatttt attgtattaa aatcatcttc tgtaatatct tttacacaat
     4681 ctgcatttgt taattctttg gtttcaatca aatatgaaca aaaaaaatta atatcttcta
     4741 aataagcaag tcgtgtagaa actgcaacag aaccttttaa atatataaaa taatccttca
     4801 taaatacagg aagtttattt tccaaaagta gacatttttc tatgagttca ttttctttga
     4861 taacaatatt atcaggttta tctgatttat tatgtacttt tataattttc ttatcattat
     4921 tcaaaattaa acctcctaaa actaaaatat aaatctttta tattattttt tgatatttat
     4981 accaagtgag tataaatatt taatttaatc attttttaca tttttaagag attagcagtg
     5041 ctttagatga aattatcact aactattccc taaatccaca tttaaagact agttgaattt
     5101 aagtactctt ttttatttct caatatattt atagaaatat tttaaaatat attgagaaat
     5161 tttttatgct ctactaaaat cttttttata taatattttt atatgtttct aacgaaacca
     5221 aatattagta aaatatgtat ttatttaact aaagttttta aatgggctga ctaagcttaa
     5281 aatataggat attatgagag ttactattga aaaaataaac cctattgcca taaagaaact
     5341 tataaatatg ttatacttaa ctattaaact ggatatattc aagttatcaa atccaaataa
     5401 gtatgtaatt gaacttatta caaaaataac tataactata aagctcagac catttaagct
     5461 attttttaaa tcagcactac taagtgacat atgtgtagat atagaataca ttaaaaacat
     5521 aaatataaaa tacttcaatg aatatacact aggtgttaac aacattattc ttataaaatt
     5581 ggaaatatca cctaaaataa tgtgtataaa cccatttata tttaaattat taatcatact
     5641 tacatataac tttaaatcta tattttgagt cactttcata taactatctg gcagtaatat
     5701 cttaaaaatt aaaataatca ttattgttcc acctataata ggtgca
//
LOCUS       DAEPIN010000139         5650 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.141, whole genome shotgun sequence.
ACCESSION   DAEPIN010000139 DAEPIN010000000
VERSION     DAEPIN010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5650
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.141"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            314..1495
                     /locus_tag="KR250_003651"
     CDS             314..1495
                     /locus_tag="KR250_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBH0069530.1"
                     /translation="MNKKYIKAGNLIDGTGNSIMQNKGIIIDGDTIVEIEDIRDDLNG
                     YDVYDYLDKTVMPGIMNCHVHLTMEPVGNPKIYYDNVSDVELVVNTIKQLDAYLDSGV
                     TYIRSLGCPKYVDVQLKNLIDKGSIDGPGIVTSGPVICMTGGHGYYFGIESDGVDECR
                     KSARTVLKNGVDCVKIMATGGVTTDGVEPGSPQLTLEEMKAAVDEAIKAGKITATHAQ
                     GRTGIKNAVLAGITSIEHGVYLDDEIIDLMLERGTYLVPTVAAPYFILNAGKESGITE
                     ATLRKCEIVAIPHKESFLKAYKKGIKIAVGSDAGTSYNEHGKTYYEMKLMSDYGMDNM
                     DIIVAATKTASELLRIDKNYGTLEKGKKADVIVVNGNPVENIDVLADVLAVFKLGKKV
                     R"
     gene            complement(1637..2554)
                     /locus_tag="KR250_003652"
     CDS             complement(1637..2554)
                     /locus_tag="KR250_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069531.1"
                     /translation="MENNKCREDFRFTQEYEEDYPNTNERYYENYQVADRYYNYPNKY
                     KEPKIKQCCCKKSMREALELLRYDALRPFVNFNQFAFISDFFIVGANLVGIDLSAPPK
                     DNLSGLDGTFERFSACNCDLIDIAGRVSYPIPVPLTLEGLINTIGTIPGVAELIALID
                     AVIPPTIDLGAILDAILAAIIDFILAASTPLANVDLASLCNLKAVAFDITPADYEDFI
                     ASLGYYLDKKHYKECNCNCDCDDCCCNKGILDNLYMSNINNQVTVVAGSLVLTGVEVL
                     GKKNDVIVLGNSNDSRIYFVCVDSIDYIA"
     gene            2807..3601
                     /locus_tag="KR250_003653"
     CDS             2807..3601
                     /locus_tag="KR250_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0069532.1"
                     /translation="MIEVDKLCKSFTRVVKDDKNKSSIRKLKKVKTKKEEFFAVNNVS
                     FKVMEGEIVGILGPNGAGKTTLLRMLGGILTPTSGSINISGYDYSIDRNSAKKEIGYL
                     SGNTKLYGRLSPRELLTTFASLYEMSKEDIEESIENIVKIMDMSEFIDNRIENLSTGQ
                     TQRTSIARCLIHSPKVYIFDEPTLGLDVLSSASIIDFMKNEKLRGKTVLYSTHYMEEA
                     ETLCDKIFMIHNGKIIASGTPQSLKEESGVNNLRDVFIKLARKEDI"
     gene            3602..>5650
                     /locus_tag="KR250_003654"
     CDS             3602..>5650
                     /locus_tag="KR250_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBH0069533.1"
                     /translation="MRSKIVKYIFKKEMLDILRDKKTLFMMIVVPLLLYPLLMLLMIQ
                     VMNMSASSMNSKDINIAFNNKPNKVLVSMIEDDNLEKDTKNTSNDKGKIRVLDVDNYK
                     KSLEENKIDAYIKMEEKKSSINYQIYINSSQENSSNALKRIETVLDKYKRFTIEKTLS
                     EKGLDVQATLEPITYSTVDVAKTEEVAGYFLGQILPFILIIGVLLGSIYPAIDVMAGE
                     KERGTLETLFTLPISNLELVMGKYMAVSLSAIVTAILNILSMGLTMAFILVSGGISSQ
                     FGFGNFNYGDLVLPIITTLICICLFSMVVSAISMCVCSLAKSFKDAQNYITPLMLIIM
                     IPSYVSMIPNIVLDRVTAVIPVVNISLLIKSVLSFKSDLNLIALVLASNVAFVILAII
                     LLSKMFNSEEILFGNSKSFSFLEKRSNIKKGSIPTVSDGVILYAVGLVLLIYVGSLVQ
                     IKFGMAGIIMTQFMIISLPLLFAYYIKADFKEVFSLKVPKLKHVFGSIVLWIGGYILI
                     MIITQLILFLFPQNMEVAESLNKALFIKDSVLLNLLAIALLPALCEEMFFRGFIFSSF
                     SKSKDKNKSIKLAIICSGVLFGIMHMDFIRIIPTSILGIIFAYSVYKSGSIFVSMLLH
                     FLNNSVAVVLNHYTTGNITNLYKFVEVDFSNLNVLKFALILLISLILIMLGLRVLDRK
                     S"
BASE COUNT         2155 a          638 c          926 g         1931 t
ORIGIN      
        1 ataagtaagg gagtgtctca aaataaactt tttagctcat gagacacttt ttttatatga
       61 aatcaaatat attttcattg tttagattta ttttgataat gaaaaaaaga ttatctttat
      121 tttaagaaag acatttattt ataattatat acaaaaatat tttttaatgt ttttgtgtat
      181 aattataaca atttaattta tattaatgca cttaaatttg aaatagaatg cttatagtgg
      241 taaaattaat tattagaata ttagaaataa taaataaata atacattgta cttaaaaggg
      301 ggagaaattt aagatgaaca aaaaatatat taaagcaggg aatttaattg atggaaccgg
      361 aaatagtatt atgcaaaata agggtataat tattgatgga gatactatag ttgaaattga
      421 agatataaga gatgatttaa atggatatga tgtgtacgat tacttagata aaactgttat
      481 gccaggcatt atgaattgtc atgttcactt gacaatggag ccagttggaa atccaaagat
      541 atattatgat aatgtatcag atgtagagct tgtagtaaat acgataaaac aacttgatgc
      601 ttatttggat tcaggagtaa catatattag aagcttaggt tgtcccaaat atgtggatgt
      661 acaacttaaa aatcttatag acaaagggtc tatagatggt cctggtatag ttacttcagg
      721 tccagttata tgtatgacag gagggcatgg atattatttt ggtattgaaa gtgatggagt
      781 ggatgaatgt agaaaatcag ccagaacagt tttaaaaaat ggagtagatt gtgtaaagat
      841 aatggcaact ggaggtgtaa caactgatgg agttgagcca gggtcaccac aattaacttt
      901 ggaggaaatg aaggctgctg tagatgaagc tataaaagca ggtaaaataa ctgccacaca
      961 tgcacaagga agaactggta taaaaaatgc agtgcttgca ggaattactt ctatagaaca
     1021 tggtgtttat ttagatgatg aaataattga tttgatgctt gaaagaggaa cttacttagt
     1081 tccaactgtg gctgcaccat attttattct aaatgcagga aaagaaagtg gaataactga
     1141 agctactctt agaaagtgtg agattgttgc cattccacat aaagagtcat tcctaaaagc
     1201 atataaaaaa ggaattaaaa ttgcagttgg ttctgatgca ggaacttctt ataatgaaca
     1261 tggaaaaaca tattatgaaa tgaaactaat gtcagattat ggtatggata atatggatat
     1321 aatagttgct gctacaaaaa cagcatctga acttttaaga attgataaaa attatggaac
     1381 tctagaaaaa ggaaagaaag cagatgttat agttgtaaat ggaaatccag tagagaatat
     1441 agacgtactt gcagatgtgt tagcagtatt taaacttggt aaaaaagtta gataaatagt
     1501 ataaaaataa aaaagagtgt ctaaagaaat ttttaaataa ttatagacac tcttttttat
     1561 tagtaataat taaatctttt atactttaga aaatagattc tattttatat ttagatataa
     1621 ctctttaaaa tcattcttat gcaatataat ctatagaatc tacacataca aagtatattc
     1681 ttgaatcatt agaatttcca agtactataa catcattttt cttacctaga acttcaacac
     1741 cagttagaac caaactacca gctactacag taacttgatt atttatattt gacatataaa
     1801 gattatctag gataccttta ttacaacagc aatcatcaca atcgcagttg caattacatt
     1861 ctttgtaatg ttttttatca agatagtaac ctaaagatgc tatgaaatct tcataatctg
     1921 caggtgtaat atcaaatgca acagctttaa gattacacaa tgatgctaaa tctacgtttg
     1981 ctaatggagt agatgcagca agtataaaat caattatagc agcaagtatt gcatctaata
     2041 tagccccaag gtctatcgta ggaggaataa ctgcatcaat aagtgcaatt aattcagcta
     2101 ctcctggtat agttcctata gtattaatta atccctcaag agttaaaggg actggaatag
     2161 gataagatac tctaccagct atatctatta aatcacagtt acaagcagaa aatctttcaa
     2221 aagtaccatc aagtccagat aaattatctt ttggaggagc tgaaagatct atacctacca
     2281 aattagcacc tactataaag aaatctgaga taaaagcaaa ttgattaaag tttacaaaag
     2341 gtcttagagc atcatatctt agaagttcta atgcttctct catacttttt ttacaacaac
     2401 attgttttat tttaggttct ttatatttat ttggataatt atagtatcta tcagctactt
     2461 gataattttc atagtatctt tcatttgtat ttggataatc ttcctcatat tcttgtgtaa
     2521 atctaaagtc ctctctacat ttattatttt ccactaaata cactcctttt atattttaat
     2581 ttaaatgtat agataattaa gcttatctat atagtaatat tatatttata atgtaaaaaa
     2641 atgttacaaa agatattata aacgaataaa aaataataat ttgccagata aacttgtaaa
     2701 tttttatgaa taatccattc taaaacaaaa aactgtgtat aaaaaaggtt tatatgttat
     2761 atttatataa atataacata tgaacgagaa aggagtggtt tgataaatga ttgaggtaga
     2821 taaattgtgt aagtctttta caagggttgt aaaagatgat aagaataaaa gttcaataag
     2881 aaagctaaag aaagtaaaaa ctaaaaaaga agaatttttt gctgtaaata atgtttcatt
     2941 taaagttatg gaaggtgaaa ttgttggaat attaggtcca aatggtgcag gaaagactac
     3001 attacttaga atgttaggtg gcatattaac accaacatca ggaagtataa atatttctgg
     3061 atatgattat tcaatagata gaaattcagc aaaaaaagaa ataggttatc tctctggaaa
     3121 tacaaaacta tatggaaggc tttctccgag agaacttcta acaacttttg caagtctata
     3181 tgagatgtca aaagaagata tagaagaatc aattgaaaat attgtaaaaa taatggatat
     3241 gagtgaattt atagataata gaattgaaaa tctatcaact ggtcagactc aaagaacatc
     3301 tatagctcgt tgtttaatac attctccaaa agtatatatt tttgatgagc caacactagg
     3361 tctggatgtt ttaagtagtg catctattat agattttatg aagaatgaaa aactaagggg
     3421 aaaaactgtt ctttactcaa ctcattatat ggaagaagca gaaactttat gtgacaagat
     3481 ttttatgatt cacaatggga aaataattgc atcaggaaca ccacagagtt taaaagagga
     3541 atctggagta aataatttga gagatgtatt cataaaactt gccagaaagg aggacattta
     3601 aatgagaagt aagattgtaa aatacatatt taaaaaagaa atgttagaca tattgagaga
     3661 taaaaagact ttatttatga tgattgttgt gccacttctt ttatatcctt tacttatgtt
     3721 attaatgatt caagtaatga atatgtctgc aagtagtatg aatagtaagg acattaatat
     3781 agcttttaat aataaaccta ataaggtttt agtatccatg attgaagatg ataatttaga
     3841 aaaagatact aaaaatactt caaatgataa aggcaaaata agagtgttag atgtagataa
     3901 ctataaaaag tcattagaag aaaataaaat tgatgcttat ataaagatgg aagaaaaaaa
     3961 atcatctata aattatcaaa tatatataaa ttcttctcaa gaaaattctt ctaatgcact
     4021 aaaaagaatt gaaactgttt tggataaata taaaagattt accatagaaa aaactttatc
     4081 tgaaaaagga ttagatgtac aagctacttt agaaccaata acatatagca ctgtagatgt
     4141 tgcaaagacg gaagaagtgg caggatactt tttaggtcag atacttccat ttatattaat
     4201 aataggagtt ttactaggtt caatatatcc agcaatagat gttatggctg gagaaaagga
     4261 aagaggtaca ttagaaacat tatttacact gccaatatca aacttagaac tggttatggg
     4321 aaaatatatg gcagtttctc ttagtgcaat agtaacagca attttaaaca ttttatctat
     4381 gggactgact atggcattta ttttagttag tggaggtatt agtagccaat ttggctttgg
     4441 aaactttaat tatggagact tagtcttacc aattataact actttaattt gcatatgtct
     4501 tttttcaatg gtagtttcag caataagtat gtgtgtatgt tcattagcaa aaagttttaa
     4561 agatgctcaa aactacataa cacctcttat gttaattatt atgataccat catatgtttc
     4621 tatgatacca aatatagtat tagatagagt gacagctgta atacctgtag taaatatatc
     4681 tttacttata aaaagtgtat tatcttttaa aagcgattta aatttaatag ccttagtttt
     4741 agcatcaaat gtagcctttg ttatattggc aatcatacta ctgtctaaga tgttcaattc
     4801 tgaggaaata ctttttggta atagtaagag tttctctttc ttagagaaaa gaagtaatat
     4861 aaaaaaaggg tctataccaa ctgtaagtga tggtgttatt ttatatgcag taggattagt
     4921 tctattgatt tatgttggtt ctttagttca aattaaattt ggtatggcag gtattataat
     4981 gacacaattc atgataattt ctttaccgtt attatttgca tactatatta aagctgattt
     5041 taaagaggta tttagcttga aagttcccaa attaaagcat gtgtttggga gcattgttct
     5101 ttggattggt ggatacatac ttattatgat tataacacag ctaattttat tcttatttcc
     5161 tcaaaatatg gaagttgctg aaagtttaaa taaggctctt tttatcaaag atagtgtttt
     5221 actgaattta ctagcaatag ctttattacc agctttatgt gaagaaatgt tttttagggg
     5281 atttatattt tcttcattta gtaaatcaaa agataaaaat aagtctatca agctagctat
     5341 aatatgcagt ggggtactat ttggtattat gcatatggat tttattagaa taattccaac
     5401 atcaatatta ggtattatat ttgcatatag tgtgtataaa tctggttcaa tatttgtatc
     5461 aatgttattg cattttttaa ataatagtgt agcagttgtg ttaaatcatt atacaactgg
     5521 aaatataacc aacttatata aatttgttga agttgatttt tcgaacttaa atgtacttaa
     5581 atttgcctta attcttctaa taagtcttat attaattatg ttaggattaa gagttttaga
     5641 tagaaagtct
//
LOCUS       DAEPIN010000140         5478 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.142, whole genome shotgun sequence.
ACCESSION   DAEPIN010000140 DAEPIN010000000
VERSION     DAEPIN010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5478
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.142"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            complement(137..820)
                     /locus_tag="KR250_003655"
     CDS             complement(137..820)
                     /locus_tag="KR250_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HBH0069534.1"
                     /translation="MTSKSQMSESFFLCSLLAITGGFLDIYTYVVRGKVFANAQTGNI
                     VLFGLNIAEGNVKESFYYLIPILAFMLGVFIAEKIRKYFTNNKTKIHWRQVIIIIEMI
                     TILIVSFIPRGRFNMFVNVAISFICSMQVESFRKVNGNSFATTMCTGNLRSATETLFH
                     YIHTKNIFMIKKSLQYYAVIIFFISGAILGTFFTKIFVEKSILICFFILAVVFGIMFI
                     NKDDSFNEN"
     gene            complement(996..1586)
                     /gene="zapA"
                     /locus_tag="KR250_003656"
     CDS             complement(996..1586)
                     /gene="zapA"
                     /locus_tag="KR250_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HBH0069535.1"
                     /translation="MNKVMVKIHGAEYPMVGDKSEKFMISIADFVDKEMDKITRQNPK
                     LSLSVAAILTALNISDLLFECSDENEKLIKANEELSKKVGASNEELQLEIKSLKLTIA
                     EKEAENREAETKMKELIEIIENKKQEIFELSNTTEGSRAELDAYKNKIEELSTQLEEA
                     NERATIAENLASEFQNKAYDLQLKCTGLNNDAKNVE"
     gene            complement(1611..4004)
                     /locus_tag="KR250_003657"
     CDS             complement(1611..4004)
                     /locus_tag="KR250_003657"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HBH0069536.1"
                     /translation="MLVSLKWLRDYVDIDMDVKEFADKMTMTGTKVETIDYYGEEIEN
                     ILVGKILEIKQHPNADKLVVTKVDIGDKVVQIVTGATNISEGDYIPVAVNGSKLPGGV
                     EIKQTDFRGELSDGMMCSAAELGIDEHYIEEYKRGGIYILDHEDSYELGKDIKDVLGL
                     KDALIDFELTSNRPDCKCMMGIAREAAATIGTKVKYPEIEVKESDEEIDFKVEIDNPD
                     LCRRYVARMVTDVKIEPSPYWMQRRLTEAGVRPISNIVDITNFVMLELGQPLHAFDIN
                     QVETGRIVVRNAKDGEKLVTLDDVERTLDKDMLVITNGEKSLGLAGVMGGANSEITSN
                     TKTVLFESANFKPENIRMTAKKVGIRSEASSRNEKDLDPNLAEIAANRAAQLVEMLGA
                     GKVLKGVVDVYPNKPEPKKLVVNPQRINHLLGVDVPMEQFVGILESLEFKCNLVANDK
                     LEIDVPSFRTDMEQEADVWEEIARIYGFENIPSVQLEGNTTAGIKTSKQKFMDALKDN
                     STAVGLNEILTYSFVSPKGVDKIRVPEGNAKRNFVKLLNPLGEETSVMRTTLIPNMLD
                     VLSTNVSHKIEEVSAFECGHIFIPQDSELPKEENRMCVGMYGKDVDFFALKGTIETIL
                     VNVGFKGYEIEPQDNNTTFHPGRCAKIVYNNKYVGTLGELHPDVIENYNLGQRVYVAE
                     IDIDFVFDNSDRTKNYVPLPKYPSTSRDIALIVKDDVFVKQIEDIIKENGQGLVESYK
                     LFDVYKGSQIEAGYKSIAYSITYRSKDKTLTDEDVAKVHDKILSELSEKLNANLRSN"
     gene            complement(4021..5040)
                     /gene="pheS"
                     /locus_tag="KR250_003658"
     CDS             complement(4021..5040)
                     /gene="pheS"
                     /locus_tag="KR250_003658"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HBH0069537.1"
                     /translation="MQEKLLALREAALAEIKEAQSIESVESLRVKYLGKKGEITAILK
                     EMGKLSAEERPVVGKVANEVRENIELSINSKKEEINAIEKERKLKEEVIDVTQPGKVL
                     KVGKKHPITQIIDEVTDIFIGMGFSIAEGPEVETVENNFDALNAPKDHPSRDMSDTFY
                     INDGVLLRTQTSPVQVRTMRSQELPIKVIAPGRCFRSDSPDATHSPMFHQIEGLVVGK
                     DVTMAEFKGTMDIFVEKLFGSDIKTKFRPHNFPFTEPSAEVDVTCFKCGGKGCPMCKY
                     EGWIEILGSGMVHPNVLRNCGIDPEVYSGFAFGVGVERLAMLKYEIDDIRLLFENDMR
                     FLNQF"
     misc_binding    complement(5090..5324)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT         1667 a         1077 c          597 g         2137 t
ORIGIN      
        1 ttactatact taaaatactt ctattaactt atacattgct tactatattt taaattcttt
       61 aattaatcta tgcaatattt gctatatata aagtatatca aataacagct tcattttttt
      121 gtaaatttac agaaagttaa ttttcattaa atgaatcatc tttattgata aacataattc
      181 caaagaccac tgccaaaata aaaaaacata ttagtattga tttttccacg aaaatttttg
      241 taaaaaaagt tcctaaaata gctccagata taaagaatat aataacagca taatactgta
      301 aactcttttt tatcataaaa atattcttag tatgtatata atgaaataaa gtttctgtag
      361 cacttcttaa atttccagta cacattgtcg ttgcaaaact attcccattt acttttctaa
      421 aactctctac ttgcattgaa caaataaaag aaatagcaac atttacaaac atattaaatc
      481 ttcctctagg aataaatgaa acaataagta ttgtaatcat ttcaattata atgataacct
      541 gacgccaatg aatctttgtc ttattatttg taaaatactt tcttatcttc tcggctataa
      601 atacacctaa cataaatgcc aaaataggaa tcaaataata aaatgattcc tttacattac
      661 cttctgctat attaagccca aacaaaacta tatttccagt ttgtgcatta gcaaagactt
      721 tacctcttac cacataagta taaatatcca aaaatcctcc tgttattgcc aacaaagaac
      781 ataaaaagaa tgattcagac atctgactct tgcttgtcat atataaacct ctcgctttct
      841 aatgatagat acaaaatcaa aatcatttta gattctattt ttcattgata taaaaaaatt
      901 atttatattt aaatattgtc tatatttata catcatttat atttatacat taaaagaagc
      961 tgtctcaaaa aacaagagac agcaaatctt ttagtttatt ccacattttt tgcatcatta
     1021 tttaaccctg tacattttaa ctgtaaatca taagctttgt tttgaaactc agaagctaag
     1081 ttttcagcta tagtagctct ttcatttgct tcttcaagtt gagtacttaa ctcctcaatc
     1141 ttgtttttat aagcatctaa ctctgctcta gaaccttctg tcgtattact aagttcgaat
     1201 atttcttgct ttttattttc tataatctca attaactctt tcatcttagt ttcagcttca
     1261 cgattttctg cttctttttc tgctatcgta agctttaaag acttgatttc taattgaagc
     1321 tcttcattag atgctccaac cttcttactc aattcttcat tagctttaat caacttctca
     1381 ttctcatctg aacattcaaa taaaaggtct gatatattta atgcagttaa tatcgcagca
     1441 actgataagc tcaatttcgg gttttgacgt gttattttat ccatttcttt gtctacaaaa
     1501 tcagcaatac taatcataaa cttctctgat ttgtctccaa ccattggata ttcagcgcca
     1561 tggattttaa ccattacttt gttcatccta acctcccccg acattagttg ttaatttgat
     1621 cttaagttag catttaattt ttcgcttaat tcacttaata tcttatcatg caccttagct
     1681 acatcttcat ctgttagagt tttatcttta cttctataag ttattgaata tgcaattgac
     1741 ttgtatccag cttctatttg agaacctttg taaacatcaa ataacttgta gctttctact
     1801 aatccttgac cattttcctt tattatatct tctatttgtt ttacaaatac atcatcttta
     1861 acaataagag ctatatctct tgatgtagat ggatattttg gtaatggcac ataattcttt
     1921 gttctatcag aattatcaaa tacaaagtca atatctattt cagcgacata aactctttga
     1981 cctagattgt agttttctat aacatctgga tgcaattcac ctaatgtacc tacatattta
     2041 ttattataca ctattttagc acatctacca gggtggaagg tagtattatt atcttgaggt
     2101 tcaatttcat aacctttaaa tccgacattt acaagtatag tttctatagt tccttttagt
     2161 gcaaagaagt caacatcttt accatacata cctacacaca ttctattttc ttccttaggt
     2221 aattctgaat cttgtgggat gaatatatgt ccacactcaa atgcagatac ttcttctatt
     2281 ttatgagata cattagttga taaaacatct aacatatttg gtattaaagt agttctcatt
     2341 acagaagttt cttctcctaa tgggttaagt aatttaacaa aatttctctt tgcattacct
     2401 tctggtactc ttatcttatc tactccctta gggcttacaa atgaatatgt taatatttca
     2461 tttaatccta cagcagttga attatctttt aaagcatcca tgaatttttg ttttgaagtt
     2521 ttaattcctg ctgtcgtatt tccttctaat tgtacagaag gtatattctc aaatccataa
     2581 attctagcta tttcttccca tacatcagct tcttgttcca tatctgttct aaagcttggt
     2641 acatctattt ctaatttatc attagctacc aaattacatt taaactctaa tgattctaaa
     2701 attcctacaa actgctccat tggtacatct acacctaata gatggttaat tctttgagga
     2761 tttactacca attttttagg ttctggttta tttggatata catctacaac accttttaaa
     2821 acttttcctg ctcctaacat ttcaacaagc tgtgcagctc tatttgctgc tatctctgca
     2881 agattagggt ctaagtcttt ttcatttctt gaagatgctt ctgacctaat accaactttt
     2941 ttagctgtca ttcttatgtt ttctggtttg aaattggcac tttcaaaaag tacagtcttc
     3001 gtattagaag ttatttctga gttagcacca cccattacac cagctaaacc aagtgatttt
     3061 tctccatttg ttataactag catatctttg tctaatgttc tctcaacatc atctaatgtt
     3121 acaagtttct ctccatcttt agcatttctt actactattc ttccagtctc tacttgattt
     3181 atatcaaaag catgaagtgg ttgaccaagc tctaacatta cgaagtttgt tatatcgact
     3241 atgttactta taggtcttac tcctgcttct gtaagtcttc tttgcatcca atatggagaa
     3301 ggttctattt ttacatctgt aaccattcta gcaacatatc ttctacataa atctggatta
     3361 tctatctcaa ctttgaaatc tatctcttcg tcactttctt ttacttcgat ttcaggatat
     3421 tttacttttg ttcctatagt tgcagctgct tctctagcta tacccatcat gcatttacaa
     3481 tcaggtctgt ttgaagttaa ttcaaaatct attaaagcat cttttaatcc taaaacatct
     3541 tttatatctt ttcctaattc ataagaatct tcgtggtcta aaatatatat accacctctt
     3601 ttatactcct caatgtaatg ttcatctata cctagttcag ctgctgaaca catcatacca
     3661 tctgataatt cacctctgaa atcagtctgt ttgatttcaa ctcctccagg taacttagaa
     3721 ccatttacag ctactggaat ataatctcct tctgatatat ttgtagctcc tgtaactatt
     3781 tgaacaactt tatctccaat atctactttg gttacaacca acttatcagc atttggatgt
     3841 tgttttattt ctaaaatctt tccaaccaat atattttcta tttcttcacc ataataatct
     3901 attgtttcaa ctttagttcc tgtcattgtc attttatcag cgaactcttt tacatccatg
     3961 tctatatcaa catagtctct aagccatttt aaagatacta acatctacat accctcctaa
     4021 ttaaaattga tttaagaatc tcatatcatt ttcgaataat aatctaatat catctatttc
     4081 gtatttaagc attgcaagtc tttcaacccc aactccaaat gcaaatccac tgtaaacttc
     4141 tgggtctatt ccacaatttc taagcacatt tggatgaacc atacctgaac ctaatatttc
     4201 tatccaacct tcatatttac acattgggca acctttacca ccacatttga aacaagtaac
     4261 atcaacctct gcacttggtt ctgtaaatgg gaagttgtga ggtctaaact tagttttgat
     4321 atcagaacca aacaattttt caacgaagat atccatagtt cctttaaatt ctgccatagt
     4381 aacatctttt ccaacaacaa gcccttctat ctgatggaac attggtgagt gtgtagcatc
     4441 tggcgagtct gacctaaaac atctacctgg tgcaattact tttattggta actcttgact
     4501 tctcatagtt cttacttgaa ctggagatgt ttgagttcta agtaataccc catcattgat
     4561 atagaatgta tcgctcatat ctcttgatgg atggtcttta ggagcgttta atgcgtcaaa
     4621 gttgttttca acagtctcaa cttctggccc ttctgctata gagaatccca ttccgataaa
     4681 tatatctgtt acttcatcta taatttgagt tattggatgc ttcttcccaa cctttaaaac
     4741 ttttcctggt tgagtaacat ctatcacttc ctcttttaat tttctttctt tttcaatagc
     4801 atttatttct tcttttttag aatttatgct aagttcaatg ttttctctta cctcattggc
     4861 aaccttacca actactggtc tttcttcagc agataattta cccatttctt taagtatggc
     4921 agttatctca cctttttttc ctaagtactt aactcttaaa ctttctacac tttctatgct
     4981 ttgtgcttct tttatttcag ccaaagctgc ttcacgtaaa gcaagtaatt tttcttgcac
     5041 acatatcacc ctttcgtttt tttgtataaa aaaagcccct caatccctgt ctagggacga
     5101 aaggctatag tttcgcggta ccaccctagt agtttaacaa tttaaaaatc atcaaaccac
     5161 ctctaacaaa cttaacgcgt ttttgacgtc aaagcctact gtaaaatttc agctctgtta
     5221 ctccagaggg aacttctcat gacctcttag aaaacactcc cagcctaggt gtttatctct
     5281 ttactaagtt taatcaaaat actttcctct ttcattgcat ttacaatatt atattgttta
     5341 ttttagtatt atatcatttc cattcaaatt atgtgactat ttttaatata tatccaatac
     5401 ataattaatt taattatttt ttatataata tacatctaat gcataattaa ctcaattatt
     5461 ttttatataa tatacatc
//
LOCUS       DAEPIN010000141         5253 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.143, whole genome shotgun sequence.
ACCESSION   DAEPIN010000141 DAEPIN010000000
VERSION     DAEPIN010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5253
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.143"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            366..1673
                     /locus_tag="KR250_003659"
     CDS             366..1673
                     /locus_tag="KR250_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069538.1"
                     /translation="MKEIADAITSEYLSYGINEDISIYEGRFCIYLDKKYRCNGKIYY
                     KMTPPISISFKADIGCVEEIDNEDDNLALDYDNAILEVHGYKIISITINTLSEFSVEG
                     YINDDCIKSKNSYVEYVDFNIINLDKIPGKLIKYNDKVYAGRIEFDINDYVVTIDKRY
                     DYRKELKSELKSKSGAIITHIGRIRRKDGRIFRTNNTINLLDRISTALSFMCGRYVGF
                     CLAKGYRSGNEVYRIWNENQISPFRYVPTWSDTLSNYYNMEKYISLMCKKLEDFYYGS
                     AIKSVVDWYIESLGSATMENNIISVQIALETLSYVILVEQNKILTDEVFDCNLASKNI
                     RLLLDTCKIPYGKHELNIFDNIIKNKFDDGVDLVIYLRNSIVHPSRKTHRAVLEVEDI
                     WNIISIGTRYIELVLLFILGYRGEYSNRLVERCYGEVEVVPWN"
     misc_binding    1759..2021
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2147..2950
                     /locus_tag="KR250_003660"
     CDS             2147..2950
                     /locus_tag="KR250_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HBH0069539.1"
                     /translation="MKNILKKVGIFTIMLGLLGGVVGCSKPDNEKDKDASKESKKEVV
                     VGFDNTFVPMGFLDEKGDTVGFDVDLAKETFKRLGMEVKFQPIDWSMKETELNDSKTV
                     DVLWNGYSITDERKKIVSYTEPYLQNKQIIVTLSDSKINSKADLKDKEVGTQQGSTAL
                     DAVEKDKDFMNSLKGGAPVLYDTYDKALRDLEIGRTSAVVGDEVLIRYYMGQKGEDKY
                     KVLKDDFGLEDYVVATSKENPELCEKINETLKEMKKDGTFDKIYDKWFK"
     gene            2988..3599
                     /locus_tag="KR250_003661"
     CDS             2988..3599
                     /locus_tag="KR250_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBH0069540.1"
                     /translation="MLQGLEIVIAMFCITLIVSIPLGIGVAFLRLSKNKLVSGITQCY
                     ILIMRGTPLLLQMIVIFYGLPLLGIVFDRFTAGVVAFFLNYAAYFAEIFRGGIQSIDR
                     GQYEASKVLGFDKFTMYKRVIFPQVFKRILAPISNEVITLVKDTSLVYILGLNDILRI
                     SQIAMNREASLLPLFEAGAIYLIFVAILTKGFELLEKKYSYYR"
     gene            3631..4302
                     /locus_tag="KR250_003662"
     CDS             3631..4302
                     /locus_tag="KR250_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBH0069541.1"
                     /translation="MLKIKNLNKSFKKNRVLKDISFELEEGQIGVLLGKSGAGKTTIL
                     RCINGLEEFDSGEIIIDNEVIKNKRDMAKIRGKIGMVFQNFNLFPHMTVLENIIESPV
                     NVFKVPRKEAEERARELLRLVDLEDKLNSYPFELSGGQQQRVAIARSCALMPKVLCFD
                     EPTSALDIDTIQRVVNIMNRLKDKGMTILIITHDVVFSNNVADKIISIKDGIVENVQI
                     KEKIV"
     gene            4600..4962
                     /locus_tag="KR250_003663"
     CDS             4600..4962
                     /locus_tag="KR250_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HBH0069542.1"
                     /translation="MIKLYGYTKCSTVKKAKNWLKENNLEFEDIDMVQNPPSKEELKS
                     IYKTSGYDIKKFFNTSGMKYRELGLKDIVKTESDDKLLEILVSDGMLIKRPLLLDGKN
                     VLLGFKEDVWKSTLLKED"
BASE COUNT         2120 a          471 c          912 g         1750 t
ORIGIN      
        1 taaaatatgt tgtaaattaa atgttttctt tgacaatata tacacaagga tgataatatc
       61 tatatgaata aaagatagtg tgtaatttga taatatgtaa ttataacatt atgatataat
      121 tacaataaag tatgtaataa tctgtagaaa tttcaagcta gttatctaag ttttcggata
      181 actaaatcta aacggaatac ataatgtatt aaataaatga tgatactaaa atgatactat
      241 ttttatagaa atttattgag gtatataatt aatgaaaata atatattaaa ttatataaat
      301 taaattatat aaattaaatt atataatata ttgagtttta cataaaagca aaggtgggaa
      361 taaaaatgaa agaaattgct gatgctataa catcagagta tttaagctat ggtatcaatg
      421 aagatattag tatctatgaa ggtagatttt gcatatattt agataaaaaa tatagatgta
      481 atgggaaaat ttattataaa atgacacctc caatatctat aagttttaaa gcagacatag
      541 gttgtgttga agagatagac aatgaagatg acaatttagc tttagattat gataatgcta
      601 ttcttgaagt tcatggatat aaaattattt ctataacaat aaatacttta agtgaattta
      661 gtgtagaagg ttatatcaat gatgactgca taaaatctaa gaactcctat gttgaatatg
      721 tagattttaa tataataaac ttagataaaa tacctggaaa actcattaaa tacaatgata
      781 aggtatacgc aggtagaatt gaatttgata taaatgatta tgttgtaact atagacaagc
      841 gatatgatta tagaaaagaa ttaaaatcag aattaaagtc taaatctgga gcaataataa
      901 ctcatatagg taggattaga agaaaagatg gtagaatttt tagaacaaat aatacaataa
      961 atcttttaga taggatatca acagctttga gttttatgtg tggaagatat gtagggtttt
     1021 gtttggccaa agggtataga agtggtaatg aagtttatag aatatggaat gaaaatcaaa
     1081 tatctccttt tagatatgta ccaacatggt cagatacact ttcaaattat tataatatgg
     1141 aaaaatatat aagccttatg tgtaaaaagc tagaagattt ttattatggt tctgctataa
     1201 agagtgttgt tgattggtac atagaatctc ttggaagtgc taccatggaa aacaatataa
     1261 tatctgtaca aattgcactt gaaactttgt catatgtaat tctagtggaa caaaataaga
     1321 tacttacaga tgaagtattt gactgtaatt tggcatctaa aaatataaga ctcttacttg
     1381 atacatgtaa gataccatat ggtaaacatg aattaaatat atttgataat ataataaaaa
     1441 ataaatttga tgatggagtg gatttagtta tttatttaag aaatagcata gttcatccaa
     1501 gtagaaagac tcatagagct gtgttagagg ttgaagatat ttggaatata atttcaatag
     1561 gaacgagata tattgaactt gttttactct ttattttagg ttatagagga gaatattcaa
     1621 acagattagt agaaagatgt tatggtgaag tggaagttgt accttggaac tagattttta
     1681 tatttatttt aaaatatata ttgacatata ttgccttatg ctataaaatc aatatcataa
     1741 taataattca gaataacatt aaatgctaag aaaagaaata gtaaaagtag ttaatcttaa
     1801 acagagagtt tccgattggt gagaggagca atagatttta ctttgaacac atctttgagc
     1861 aggactctga aagagtagaa atactttatt aggagatttc cggaatcctt gcacccgtta
     1921 tagtgctaga gtataataat tttattgtac ttaaagaggt tagtgtcgtg aggcattaat
     1981 gaacagaggt ggaaccacga gagttaactc ccgtcctcta gcagaagcta gaggcgggag
     2041 ttttttaata taatttttta agcgcgcaga gaattatatt gcaatagata cactttttaa
     2101 aaaataatca aaatatttta aaaattaaaa ggcgagggag aatattatga aaaatatatt
     2161 aaaaaaagta ggaatattta caattatgtt aggtttatta ggtggagttg taggttgttc
     2221 aaaaccagat aatgaaaaag ataaggatgc ttcaaaggaa tctaaaaaag aagtagttgt
     2281 agggtttgat aatacgtttg taccaatggg atttttagat gaaaagggag atacagtagg
     2341 atttgacgta gacttagcta aagaaacatt taaaagactt ggaatggaag ttaagtttca
     2401 gcctatagat tggtcaatga aagaaactga attaaatgat tcaaaaactg tagatgtatt
     2461 atggaatgga tattctataa cagacgaaag aaagaaaata gtatcatata cagaaccata
     2521 tttacaaaat aagcagataa tagttactct aagtgactca aaaataaatt caaaagctga
     2581 tttaaaagat aaagaagttg gaacacaaca aggttcaaca gcacttgatg cagtggaaaa
     2641 ggataaagat tttatgaata gcttaaaagg tggagcacca gttctttatg atacttatga
     2701 taaagcactt agagatttag agataggtag aactagtgca gttgttggtg atgaagtttt
     2761 aattagatac tatatgggac aaaagggtga agacaaatac aaggtattaa aagacgattt
     2821 tggattagaa gattatgttg tagcaacttc taaagaaaat ccagaacttt gtgagaaaat
     2881 aaatgaaaca ttaaaagaaa tgaaaaaaga tggaactttt gacaagattt atgataagtg
     2941 gtttaaatag aagataagtt agctgttaag aagggggcac taaaggcatg ttacaaggtt
     3001 tagagatagt tatagcaatg ttttgtataa cattgatagt atcaattcca ttggggattg
     3061 gagttgcatt tttaagatta tcaaagaata aattagtaag tggtattaca caatgttata
     3121 tattgataat gagaggtaca cctttacttt tgcaaatgat agtcatattt tacggattac
     3181 ctcttttagg aattgtattt gacagattta cagctggagt agtagctttc ttcttaaatt
     3241 atgcagcata ttttgcagaa atatttagag gtggaataca gtcaatcgat agaggacaat
     3301 atgaagcttc aaaagtacta ggatttgata agtttactat gtataagaga gtaatctttc
     3361 cacaagtttt taaaagaata ctagcaccta tttcaaatga agtaatcaca ttagtaaaag
     3421 atacatcttt agtatatata ttaggattaa atgatatttt aagaatatct caaatagcaa
     3481 tgaacaggga agcaagtctg ttaccattat ttgaggcagg agcgatatat cttatatttg
     3541 tagctatact tactaaggga tttgaattac ttgaaaaaaa atattcttat tatagataaa
     3601 gttacaatat tataggagga ggttttttat atgttgaaaa tcaaaaactt aaataaatca
     3661 tttaaaaaaa atagagtact taaagatatt tcttttgagt tagaagaagg tcagattgga
     3721 gttttacttg gtaagtctgg tgcaggaaag accacaatat taaggtgtat aaatggtctt
     3781 gaagaatttg atagtggaga gattataata gataatgagg taataaaaaa taagagagat
     3841 atggcaaaaa tacgaggaaa aataggtatg gtttttcaaa atttcaactt atttcctcat
     3901 atgacagtcc ttgaaaatat aattgaatcc ccagttaatg tttttaaagt tcctagaaaa
     3961 gaggctgaag agagggctag agaactttta agattagttg atttagaaga taaattaaat
     4021 tcttatccat ttgaattgtc aggaggacag caacagagag ttgctatagc taggtcatgt
     4081 gcattgatgc caaaagtcct ttgttttgat gaacctactt ctgcacttga tattgatact
     4141 attcaaagag tagtcaatat aatgaacaga cttaaagaca aaggtatgac aatattaata
     4201 attacacatg atgttgtctt ctcaaataat gttgcagata aaattataag tataaaagat
     4261 ggtatagtag aaaatgttca aataaaagaa aaaattgttt agagttttat aaatataaac
     4321 tagggtagaa ttttataata taaataaagg gcaagtaaaa tcaaatatac tatgcccttt
     4381 tgttatctca aaatttaatc ttatatttta tgtttaagtt acattataat ttataataaa
     4441 aataaaagga ctatttaaat atataatgtg aataatgttt aataatttga aatatgataa
     4501 ttttttatgt tgaaagctta taatgattat gatataaatt ggttatagta tacaatttat
     4561 gggtataaca ataaaaaatt agaaaggaat ttttataaaa tgattaaatt atatggatat
     4621 acaaaatgct ctactgttaa aaaagctaaa aattggctta aggaaaataa tttagaattt
     4681 gaagatatag atatggttca aaaccctcct agtaaagagg agttaaaatc aatttataag
     4741 actagtggat atgatataaa gaaatttttt aatacaagtg gtatgaaata tagagaattg
     4801 ggtcttaaag atattgtaaa aactgaatct gatgataaac ttttagaaat attggtaagt
     4861 gatggtatgc ttattaaaag accattactt ttggatggta aaaatgtatt attgggtttt
     4921 aaagaagatg tatggaaaag tactttgcta aaagaagatt aggaagagat aggtgtgttg
     4981 attaaaatta ttaattaata cacctataaa tttatgtatt aaatctaatc tttataaata
     5041 ttaattagca aattcaaatc aaaaaaattc atatcaacaa acatattact tcttattaca
     5101 aattatatca tatgttttat attaaaatat tataatatta caacgaattg ttacaaaatt
     5161 acttattatt aaccatgaac atttgttttg tgctatcata tttatataag ggaattgtca
     5221 taaaaaatat cttgttcata cagttttata tag
//
LOCUS       DAEPIN010000142         5026 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.144, whole genome shotgun sequence.
ACCESSION   DAEPIN010000142 DAEPIN010000000
VERSION     DAEPIN010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5026
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.144"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     misc_binding    121..352
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            460..1941
                     /locus_tag="KR250_003664"
     CDS             460..1941
                     /locus_tag="KR250_003664"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HBH0069543.1"
                     /translation="MYYSSTRGTEEKVTASQAIIKGISNDGGLYVPSEFPNVKNELIN
                     LVNLTYSQIAFFVLSKFLCDFTEDEIKNCIENAYDEKFDCSSIAPLNKVNDTYFLELY
                     HGPTLAFKDMALTIMPHLLKTSIKKDNLEKDVVILTATSGDTGKAALEGFKDIDKIKI
                     IVFFPEDGVSPVQKLQMKTQTGKNTYVVGIKGNFDDAQSGVKKIFSDKEFNQKLLDNN
                     YILSSANSINIGRLLPQVAYYFYSYMKLVSNSDINLNDKINFVVPTGNFGNILAGYYA
                     KQMGLPINKLICASNDNNVLYDFFKTGVYDKNRTLKLTTSPSMDILISSNLERLLFEI
                     SNRNTDIVNKLLSDLSDKGVYKISDDMEKNLASFYGEYSNEEEVKSTINNVFKNYNYL
                     IDTHTAVAYNCYEKYKKETSDNTKTVIVSTASPFKFSEDVLKSIDCDFKDLDDFAIID
                     RLSSISKIDIPKPIKNLKNAEILHKDIYEKDELKLAIKKFLKV"
     gene            1951..2844
                     /locus_tag="KR250_003665"
     CDS             1951..2844
                     /locus_tag="KR250_003665"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HBH0069544.1"
                     /translation="MLEIIVPATSANIGPGFDCLGIALNIYNKFYVEEIESGLEIEGC
                     EDAYKNENNLVYTSMKYFFDRVKPEKIPAGIKIKIQSEVPICRGLGSSASCIVAGVIA
                     ANALSGANLDKNQLLNIASEIEGHPDNVAPAILGNMIVSVTDNENIHYDIIKIPEELK
                     FCAMIPNFKLSTEKARGVLPKEIPYSDGVFNVSRVALLISALLNKNFDLLKVACQDKL
                     HQDYRGTLIENYNDIVEKSEQLNSIGVFLSGAGPTIMSLIKENDDSFVDNMKNYLQKL
                     KSDWEIKELCCDSNGAVLNII"
     gene            2968..4029
                     /locus_tag="KR250_003666"
     CDS             2968..4029
                     /locus_tag="KR250_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HBH0069545.1"
                     /translation="MIIKELIIRDLVAKVPIIQGGMGVGISRSSLAGAVAKLGGVGVI
                     SGVQIGYDEEDFETNTINANLRAIKKHISKAKEISNGGIIGINFMVAMKEYETYVKEA
                     VKAGVDLIISGAGLPNKLPSLVKGSNVKIAPIVSTAKAANVILKMWDRKEKTTADLIV
                     VEGPKAGGHLGYSNEELDNIDSIDYDKEFVEILKVANTYGEKFGRNIPVVAAGGITSS
                     SDVKKYIDMGASGVQVGTRFVATYECDAHENFKMAYINASEEDVHIVKSPVGLPGRAI
                     RNKFIEEVKINRPEIKKCYNCLIPCNPKETPYCISQALINAVKGDVENSLLFCGNDAY
                     KIDKLSTVEDVINELISEL"
     gene            complement(4184..4696)
                     /locus_tag="KR250_003667"
     CDS             complement(4184..4696)
                     /locus_tag="KR250_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HBH0069546.1"
                     /translation="MSDFKEILAEEIKNNPFGLIGKDWTLITAENEGKVNTMTASWGG
                     LGVMWGKDVAFVVIRPQRYTKEFIDNTDKFSLTFFDEDFRKELSYCGKVSGREEDKIS
                     QIGFNIEHLNDTPYFKEAKMAIICKKMYSQKLEPQCFIAEGIDGRWYPQKDYHTLYIA
                     EITNVLVKED"
BASE COUNT         1879 a          618 c          769 g         1760 t
ORIGIN      
        1 ctaatactca atttgattac aattttttta caatttaaaa aatatttttt ccaaattttt
       61 atattaatac ttgactttgt ctgttttata aactagaata attaataata ttaagaatat
      121 tgaaaggcat tgattaggaa gagtaaatct gaatcgatta ccagagaagg aaactactag
      181 ctgaaatgtt tccctaatta gaaggattga aaactaccta ggagcttcta tccgaagtta
      241 tcttaatgat tttagtagga atagacgttt ttattacttg cgttaaagta tcaagtggat
      301 tttttaaaat caatttgggt ggaaccgcgg gaaacagatt ctcgtccctt ttttagggat
      361 gagggtcttt ttttattacc taaattcagt ttttaaaata ttctgtatct aatttaatta
      421 gctaattaaa aatttaaaat atatggagga ttataaaaaa tgtattattc aagtacgaga
      481 ggcaccgaag aaaaagtaac tgcatcacaa gcaataataa aaggaatatc aaatgatggt
      541 ggtctttatg ttccaagtga atttcccaat gtaaaaaatg aacttataaa tttagtaaat
      601 ttaacgtatt ctcagatagc attttttgta cttagtaaat ttttatgtga ttttactgaa
      661 gatgaaatta aaaattgtat agaaaatgca tatgatgaaa agtttgattg tagtagtata
      721 gctccattaa ataaagtaaa tgatacttat ttcttagaac tatatcacgg tccaacttta
      781 gcatttaaag atatggcact tactataatg cctcatcttc ttaaaacatc tataaagaaa
      841 gataatttag agaaagatgt tgtgattctt actgctacat ctggagatac aggtaaagct
      901 gcacttgagg gatttaaaga tatagataaa ataaagataa ttgtattctt cccagaagat
      961 ggtgtaagcc cagtacaaaa acttcaaatg aaaactcaaa ctggaaaaaa tacatatgta
     1021 gttggtataa aaggaaattt tgatgatgca caatcaggtg taaagaagat tttttctgat
     1081 aaagaattta atcaaaaact attggataat aattatatat tatcttctgc aaattcaatt
     1141 aatataggaa gacttcttcc acaagttgca tactattttt attcatatat gaaattagtt
     1201 agcaatagtg atataaattt aaatgataaa ataaattttg ttgtcccaac tggtaatttt
     1261 ggtaatattc tagctggata ttatgccaaa caaatgggtc ttccaataaa caaattaata
     1321 tgtgcatcaa atgataataa tgttctttat gatttcttta aaactggtgt atatgataaa
     1381 aatagaacat tgaaattaac tacttctcct tctatggata tattaatttc aagtaattta
     1441 gaacgattac tgtttgaaat atcaaataga aatactgata ttgtaaataa gcttttatca
     1501 gaccttagtg ataaaggagt ctacaaaata agtgatgata tggaaaaaaa tttagcctca
     1561 ttctatggag aatattcaaa tgaagaagaa gttaaaagca cgataaacaa tgtgtttaaa
     1621 aattataatt atttgataga tactcatact gctgttgcat acaattgtta tgaaaaatat
     1681 aaaaaagaaa cttctgataa cacaaaaact gttattgtaa gtactgcaag tccattcaag
     1741 ttttctgaag atgttctaaa gtctatagat tgtgatttta aagaccttga tgactttgct
     1801 ataattgata gattatcaag tatttctaaa atagatattc caaaacctat taaaaatctt
     1861 aaaaatgcag aaatactaca taaagatatt tatgaaaaag atgaattaaa actagctata
     1921 aaaaaattct taaaggtata ggtgattatt atgttagaga ttatagttcc tgcaacaagt
     1981 gctaatatag gtccaggatt tgattgtctt ggaattgcgt taaatatata caataaattt
     2041 tatgttgaag aaattgaaag tggtcttgaa atagaaggtt gtgaagatgc ttacaaaaac
     2101 gaaaacaact tagtttatac atctatgaag tacttttttg atagagttaa accagaaaaa
     2161 atacctgctg gtatcaaaat aaaaatccaa agtgaagtac ctatatgtag aggtcttggc
     2221 agtagtgcct cttgtatagt agctggagtt atagctgcaa atgctctatc aggtgctaat
     2281 ttagataaaa atcaattact aaatatagct tcagaaatag aaggtcaccc agataatgtt
     2341 gctcctgcta ttttaggaaa tatgattgta tctgttactg ataatgagaa tatacattat
     2401 gatattataa aaattcctga agaacttaaa ttttgtgcaa tgatacctaa ttttaagtta
     2461 tcaacagaaa aagctagagg tgttttacca aaggaaatac catattctga tggggtattc
     2521 aatgttagta gagttgcttt actaatatct gccttactaa acaaaaactt tgacctactt
     2581 aaagtagctt gtcaagacaa attgcatcaa gattatagag gaactctaat tgagaactac
     2641 aatgatattg ttgaaaaatc tgaacaatta aatagtattg gagtattttt aagtggagct
     2701 ggacctacta taatgtcttt aataaaagaa aatgatgata gttttgtgga taatatgaaa
     2761 aattacctac aaaaactaaa atctgattgg gaaataaaag agttatgttg cgattctaat
     2821 ggagctgttt taaatataat ataattatcg tcttctatac aaaatataac tactctttta
     2881 ttataatttg tagtataata attaggacta ggtaataata ttaagtatta taatctagtt
     2941 ctttaaacat gtttacattg aggagggatt atcataaaag agttaataat tagagattta
     3001 gttgcaaaag ttcctataat tcaaggtgga atgggcgttg gtatatctag gtcatcacta
     3061 gctggtgcag ttgctaagct tggtggtgtt ggtgtaattt ctggagttca aattggatat
     3121 gatgaggaag attttgaaac taatactata aatgcaaatt taagagctat aaaaaaacat
     3181 atttctaagg ccaaagaaat ctcaaatggt ggtattattg gtattaattt tatggtagct
     3241 atgaaagaat atgaaacata cgtaaaggaa gctgttaaag ctggtgtaga ccttataata
     3301 tctggagctg gattacctaa taagctacca tcattagtaa agggaagtaa tgtaaaaata
     3361 gcacccatag tttcaactgc aaaagctgct aatgtaattt taaaaatgtg ggatagaaaa
     3421 gagaaaacta ctgctgactt aatagttgtt gaaggtccaa aagctggtgg tcaccttgga
     3481 tactctaacg aggaacttga taacatagat tctatagact atgataagga atttgttgaa
     3541 attttaaagg ttgcaaatac ttatggagaa aaatttggta gaaacatacc agtagttgct
     3601 gctggtggta taacttctag tagtgatgtt aaaaaatata tagatatggg tgcaagtggt
     3661 gtacaagttg gtacaagatt tgttgctact tacgaatgtg atgcccatga gaactttaaa
     3721 atggcataca taaatgctag tgaagaagat gttcatatag ttaaaagtcc tgtaggtctt
     3781 cctggacgtg ccataagaaa caaatttata gaagaagtta aaataaatcg tccagaaata
     3841 aagaagtgct ataattgttt gattccttgt aatccaaaag aaactcctta ctgtatttct
     3901 caagctctaa tcaatgcagt taaaggagat gttgaaaatt cacttttatt ttgtggaaat
     3961 gatgcctata aaattgataa attaagtact gttgaagatg ttatcaatga attgatttct
     4021 gaattataat ttaaagaaaa ctaaattaaa attttatcaa gctaaggagt ttaaatacgt
     4081 tatttattaa tctttataaa catatttatg tccttagctt ttttgtgtca atttcctttt
     4141 attgaaattt taatttaatt agagatagta ttaaccctta taactaatct tcttttacaa
     4201 gtacattggt tatttcagct atatataaag tatgataatc tttttgtgga taccatcttc
     4261 catctatacc ttcagcaata aaacattggg gttcaagttt ctgactatac atttttttgc
     4321 atataatagc cattttagct tccttaaaat aaggtgtatc atttaagtgt tctatattaa
     4381 atccaatctg tgatatctta tcctcttctc taccagatac ttttccacaa tatgaaagct
     4441 ctttacgaaa atcttcatca aaaaatgtaa gagaaaattt atctgtatta tctataaatt
     4501 ccttagtata acgctgtggt cttattacta caaaagcaac atcttttccc cacataactc
     4561 caagccctcc ccagctagca gtcattgtat tcactttacc ttcattttct gctgttataa
     4621 gtgtccaatc tttacctatc aatccaaatg gattattttt aatctcttct gccaaaattt
     4681 ctttaaaatc actcatatct ctaataacct ctcattctct tatatttata ttccttttat
     4741 tttcatgata aattataaat gaagtctagt tttgaaaata aaatactttt ttaaatatat
     4801 tattgcaaat aagtaaatat atatatacac tcttattttt taatatattt aataatttca
     4861 ctcagaatat atatattctt tactcaaatt aaatataact tatatacttt atgtgtaaat
     4921 tatggtaaaa atattattta atcaaatcaa aacttgattt atatacctgg aaaaggtatt
     4981 atattaaaga gtgcttaaaa tacgcatttc ataaatagct atatat
//
LOCUS       DAEPIN010000143         4994 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.145, whole genome shotgun sequence.
ACCESSION   DAEPIN010000143 DAEPIN010000000
VERSION     DAEPIN010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4994
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.145"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            complement(555..1196)
                     /locus_tag="KR250_003668"
     CDS             complement(555..1196)
                     /locus_tag="KR250_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HBH0069547.1"
                     /translation="MNNNFQDNQTINLIQSRRSIRKFTTEQISDEQVNTLLHCAFAAP
                     SGCNKQPWHITVVQDQKLLKEISDDTLSRIHEVSNVEINKNFKLFYGAPTVLFISYDE
                     SSSWAPYDIGILTGNITTAAQALGLGSCIIGMVRGLFTPVEQGDIEGLVSVLDKEDVK
                     ESESIKMKFDTNKKYRELLDIPEGYSVPFGIAVGIPDGNLPNAREVVYKVSRV"
     gene            complement(1439..2242)
                     /locus_tag="KR250_003669"
     CDS             complement(1439..2242)
                     /locus_tag="KR250_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBH0069548.1"
                     /translation="MNKKGFTLIELLVVISIIGILVIVAVPALFRNIEKSKAVTCLSN
                     RENIKTQIVIAMAEESSKGKNEVMKEVLENKDGKYFETEPKCKSGGIYSATFDDGYDG
                     ITGIESIAKVYVTCTKHPDGVEMARDIHQSMKDLIASFSQDPSIIPGASKGNDDFRKY
                     LLDNKYKNGWPTIPDEFKAKYGLSKDTLYIQPYAYSPTKSDATVVVFANNKTGGNWYT
                     SLVYDYDEGRWYKGKNGISVAGRSWDVDTDSVKSVKTEIHSKEGWGPLN"
     gene            complement(2398..3573)
                     /gene="thiI"
                     /locus_tag="KR250_003670"
     CDS             complement(2398..3573)
                     /gene="thiI"
                     /locus_tag="KR250_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HBH0069549.1"
                     /translation="MVYNILIVKYGEIGVKGKNRYIFENRLIRNIRNMLKPIGRFNVY
                     KEYGRIYVDLEDYDYEEVIEEVRKVFGIVGVCPGVRAKKDYDTLKEIALKMLEEKIEA
                     GYKTFKVESRRGDKSFGLTSQEMSMDIGGYLLSKVGDRINVDVRNPEVKIKCEYREFH
                     TMVYSDTIPGYGGLPLGTNGRAMSLLSGGIDSPVATWMVAKRGMEVEAVHFHSYPFTS
                     ERSQEKVKDLAKILAKYCGRVRLHKVNILEIQKAIGENCNEEEATILSRRFMMRIAQR
                     LSEKRHCDALITGESIGQVASQTIQGLTCTNAVVDLPVFRPLIAMDKSDIVDIAKKIG
                     TFETSILPEEDCCSVFSPRKPVTKPRLEKIEKSETALDIEKLVQDAIDKIEVEDIEF"
     gene            complement(3619..4770)
                     /locus_tag="KR250_003671"
     CDS             complement(3619..4770)
                     /locus_tag="KR250_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HBH0069550.1"
                     /translation="MEIYLDNSATTKPYQEVIDKMVYALNTEYGNPSSVHRKGVEVEK
                     AIKEVRQDIAKSLGAKEKEIYFTSGGTECNNTIIRGITSLNKKRKNHIISTNIEHPSV
                     LNTLKDLEEDGFEVTYLEVGKDGKINIEDLKNAIKSTTCLVSMMHVNNEIGTIQPIGE
                     VGKYLKGLKEKIYFHVDAIQSYGKINFRLSKYNIDFMSVSAHKFHGPKGIGFMYIKEN
                     NRLKPMLTGGGQEIGIRSGTENVPGIYGLGEAVRILNKDLDVVISKVNNLKNILKNEI
                     IDNIEDIKINSPEDGVCHILNVSFRGTKGEVLLHYLEQKGIYVSTGSACSSKKKGSYV
                     LNAIGLTNEEINGTIRFSLSDMNTEEEMLEAVKVLKESICDLRSIMKRK"
BASE COUNT         1570 a          939 c          470 g         2015 t
ORIGIN      
        1 tactatttat tgaaaattta gatatttttc taaaaattat ctatgttaat agttaaattt
       61 atgaacattt tttgttttta tgttaggata tttcttagaa atgaaatact tcttaatttg
      121 atttgatatg tatattttta tagctatttt attataaaaa aaaagaaccc ttttataact
      181 aagggttact ttttttctct attttttctt attatatcac tattttttcc ctatttcaat
      241 attttgacat tttttccatt atactgaaaa gatatcttaa atttttaact tggataaatt
      301 ttaaagcata tacttaaaat taacatactt gttttctatt ttattacact taatccttat
      361 ttttatcatt ctacaaaaca aaaataaaaa agaataagat aataaataaa atacttatca
      421 ccttattctt tatttaatta ttttacaacg aaaaatctaa actacttttt aaatagcttc
      481 ctcttccatc aatttatttg tatattactg gtttaattct tcaagtaact gattggttct
      541 tgcaatactt tatcctaaac tctggaaact ttataaacta cctctctagc atttggtaaa
      601 tttccatctg gaatacctac agcaatacca aatggtacag aatacccttc tggtatatca
      661 agtagttctc tgtatttttt atttgtatca aacttcatct ttattgactc tgattctttt
      721 acatcttctt tatccaatac actcacaaga ccttcaatat ccccttgttc aacaggtgta
      781 aataaacctc taaccattcc tattatacaa cttccaagcc ctagtgcttg tgcagctgtt
      841 gttatattcc ctgtaagtat tcctatatca tatggagccc atgaactact ttcatcataa
      901 gaaataaata aaacggttgg agcaccataa aataacttaa agtttttatt tatctcaaca
      961 ttactcacct catgaatcct agataaagta tcatcactaa tctcttttaa aagtttctgg
     1021 tcttgcacaa cagttatgtg ccaaggctgt ttattacatc ctgaaggtgc tgcaaatgca
     1081 cagtgtaata atgtgtttac ttgctcatca ctaatttgtt ctgtcgtaaa ttttcgtata
     1141 cttctacgac tttgtattaa atttattgtt tggttgtctt gaaaattatt attcatatct
     1201 attccccttt ttatgtattt cttagtacaa atactaaatc ttcagaaaat taatcttata
     1261 attaaatttc tttacaatat tattataatt atttttagct tgtatgtgaa gacttaaaaa
     1321 tataaacact tttaatatat ctcaattata tatataaatg aaaatctggt attcgtcatc
     1381 tatagaaact gctcttttct atagtttaat caataccaga ttaataaaac atgatatatt
     1441 aatttaaagg accccatccc tctttagaat gaatctctgt ttttacagac ttaacactat
     1501 ctgtgtcaac atcccatgac ctacctgcaa cagaaatacc atttttacct ttataccatc
     1561 taccttcatc ataatcgtaa actagggaag tataccaatt acccccagtc ttattatttg
     1621 caaatacaac tacagtagca tcagatttag taggactata tgcatatggt tgtatatata
     1681 gtgtatcctt acttaatcca tattttgcct taaattcatc tggaattgta ggccacccat
     1741 ttttatattt attgtctaat aaatattttc taaaatcatc attacccttt gaggctcctg
     1801 gtattataga agggtcttgt gaaaatgatg caatcaaatc tttcatactt tgatgtatat
     1861 ccctagccat ttcaacacca tctggatgtt ttgtacaagt aacatacact tttgcaatgc
     1921 tttctattcc agttattcca tcataaccat catcaaacgt tgctgaatat attccacctg
     1981 acttacactt tggttctgtt tcaaagtact taccatcttt gttttctaat acctctttca
     2041 tgacttcatt cttgccttta cttgattcct cagccattgc aataacaatt tgagtcttta
     2101 tattttctct attagaaaga catgtaactg ctttactttt ttctatattt ctaaacaatg
     2161 ctggaacagc tactataact aaaatgccta ttatagatat aactactaac aattcaatta
     2221 gtgtaaaacc ctttttattc ataataatca ttcccattaa ataaaattat agtacttatt
     2281 tttaaatact acatattaat aaattgtcta cataaatcct ttcaacagaa ctactaatat
     2341 acttacagaa ctactaatat actcaatatt caatttagta attaaataac ttgtatgcta
     2401 aaactcaata tcctctacct ctattttgtc aatagcatcc tgtactaatt tttcaatgtc
     2461 aagtgctgtt tcagattttt ctattttctc taatctaggt ttagtgacgg gctttctagg
     2521 agaaaataca ctacaacaat cttcttcagg caatatagat gtttcaaaag tccctatttt
     2581 tttggctata tcaactatat ctgatttatc cattgctata agtggtctaa acactggtaa
     2641 gtctacaacg gcattagtac aagtaagtcc ttgtatagtt tgagatgcaa cctgcccaat
     2701 actttcacct gtaattaaag catcacaatg tcttttttca gaaagtctct gtgctatcct
     2761 catcataaat cttcttgaaa gaatagtagc ttcttcttca ttacaattct cgccaattgc
     2821 tttttgtatt tctaagatat taactttatg aagccttact ctaccacagt attttgccag
     2881 tattttagct aaatccttaa ctttttcctg tgacctttca cttgtaaatg gataactatg
     2941 aaagtgaact gcctctactt ccatacctct ttttgcaacc atccatgtag ctactgggct
     3001 atctatacct cctgataaaa gagacatagc tctaccattc gttccaagtg gcaatccacc
     3061 atatccaggt atagtatcac tatagaccat agtatgaaat tctctgtatt cgcactttat
     3121 tttaacttct ggatttctta catcaacatt aatcctatct cctacctttg ataataagta
     3181 tccacctata tccatactca tttcttgaga agtcaacccg aaagacttat ctcctcttct
     3241 tgactctacc ttaaaagttt tatatccagc ctctattttt tcttctaaca tttttaaagc
     3301 tatttctttt aatgtatcat aatctttttt agctcttact ccagggcaca cacccactat
     3361 cccaaatact tttctaactt cttcaattac ttcttcataa tcatagtctt ctaaatcaac
     3421 atatattctt ccatactctt tatatacatt aaatcttcca attggtttta acatatttct
     3481 tatgtttctt ataagcctat tctcaaatat atatctattc tttcctttta ctccaatttc
     3541 tccatacttt actattaata tattatacac catttcacct cttaaactaa tttattatca
     3601 ctatctatac agtattcttt attttctttt cattattgac cttaagtcac aaattgattc
     3661 tttcaaaact ttcacagctt ctaacatttc ttcttctgta ttcatatcag ataaactaaa
     3721 tcttattgtt ccatttattt cttcatttgt aagacctata gcatttaata cgtaactacc
     3781 tttcttttta gaagaacatg cagagccagt tgatacataa ataccctttt gctctaagta
     3841 atgaagtaaa acttctcctt ttgtccctct aaatgataca tttaatatat gacatactcc
     3901 atcttctgga gaattaattt ttatatcttc tatattatca attatttcat tttttaatat
     3961 attttttaag ttatttactt tcgaaataac aacatctaaa tctttgttta gtattctaac
     4021 agcttctcca agaccatata ttccaggtac attttctgtt cccgacctaa ttccaatttc
     4081 ctgaccacca cctgttaaca taggcttaag gcgattattt tcttttatat acataaatcc
     4141 aattcccttt ggaccatgaa atttatgtgc acttacactc ataaaatcta tattgtattt
     4201 agatagtcta aaattaattt ttccatatga ctgaatagca tcaacatgga aataaatttt
     4261 ttcttttaaa cccttcaaat actttccaac ttcacctatt ggctgtatag ttcctatttc
     4321 attgttaaca tgcatcatgc ttactaagca tgtagttgac tttatcgcat tttttaaatc
     4381 ctctatattt attttcccat cttttccgac ctctaaataa gttacttcaa aaccatcttc
     4441 ttctaaatct tttaatgtat ttaaaacaga aggatgttct atgttagtag atattatgtg
     4501 attttttctt tttttattta aactagtaat acctcttatt atagtattat tacactctgt
     4561 accacctgat gtaaaataaa tctccttttc tttcgctcct agagatttag ctatatcttg
     4621 tcttacttct tttatagctt tttcaacttc tacacctttt ctatggacag aagaaggatt
     4681 tccatattcc gtattaagtg cataaaccat tttatctata acttcttgat aaggttttgt
     4741 agttgcgcta ttgtctaagt atatttccaa cttaattaca cctaccttta tttaaaatct
     4801 tcaactctaa ctattatacc actaattata gtattttttt tgttaatctt tattaaaaag
     4861 acaatcttaa attataaaat attatttcat gaaaagctag ttttttcact ttattacaaa
     4921 tttttatagt aaaatataat atacttatac tataccctac aaaaatatat tataaaatac
     4981 aatatatgat aaaa
//
LOCUS       DAEPIN010000144         4966 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.146, whole genome shotgun sequence.
ACCESSION   DAEPIN010000144 DAEPIN010000000
VERSION     DAEPIN010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4966
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.146"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            complement(<1..334)
                     /locus_tag="KR250_003672"
     CDS             complement(<1..334)
                     /locus_tag="KR250_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator"
                     /protein_id="HBH0069551.1"
                     /translation="MKIHVEQGINCDDIEITIKCSALDNNIEKIISLLSTPLLDIKGK
                     KSGEIYKLNVCDILYFETVGNKTFAYYEDDVFEIDLKIYKLSKLLANTSFSRISKSMI
                     LNMDYIKSI"
     gene            complement(591..740)
                     /locus_tag="KR250_003673"
     CDS             complement(591..740)
                     /locus_tag="KR250_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvrJ family protein"
                     /protein_id="HBH0069552.1"
                     /translation="MDDSLQLLITNVGFPIALSMYLLVRIEGKLNTLSNSIDTLSKNI
                     LIFKK"
     gene            complement(753..983)
                     /locus_tag="KR250_003674"
     CDS             complement(753..983)
                     /locus_tag="KR250_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2922 domain-containing protein"
                     /protein_id="HBH0069553.1"
                     /translation="MEIKKKLVMSFKNVKDKQVSFSIENPKDDLTEENIKSVMDLIVS
                     KNIFSVGGFDLASVVEAKVVETNTTPYDLVIE"
     gene            complement(1801..2022)
                     /locus_tag="KR250_003675"
     CDS             complement(1801..2022)
                     /locus_tag="KR250_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1659 domain-containing protein"
                     /protein_id="HBH0069554.1"
                     /translation="MEVVSTKNLSSIKLKLDAGFDDKGKAVVKSKSFANVKAEALNED
                     VYAVAEAIASLQENPIVDILKLDSTSLSK"
     gene            2602..2859
                     /locus_tag="KR250_003676"
     CDS             2602..2859
                     /locus_tag="KR250_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBH0069555.1"
                     /translation="MKIKVEDIPVQFHAMVEIIGIDKFIEVAKLYGGTNTYIPTYKGL
                     FRYARNRDIVKQFNGVNHNELAIRYNMCVSNIKRILNENNV"
     gene            3328..4566
                     /locus_tag="KR250_003677"
     CDS             3328..4566
                     /locus_tag="KR250_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conserved phage C-terminal domain-containing
                     protein"
                     /protein_id="HBH0069556.1"
                     /translation="MNKGFYKLNSVVLNFDIKNDVNKIRFNHYIMRKENIQRLQGTLP
                     NGQFYMTVRRAATDLQLSISTISRLVKEFLDLGIIRLVSKGVKGNRSVYSYVSIEFNG
                     SLFDDSKYMELDCVCNDEFIDNSGIEFDCDSKDNSFNGDINVETVHCINEYLSDDDVE
                     HNYNNFHNKDTIKDKAISNISTIKKYIDESFDKNKSENSNLQQESINFNFNDTCNEID
                     KRTNNSIENNQNKRPMRLSDISLLSDKGMTNKKNYFNHNHGIQKGTKSVTKKKESLNK
                     FNKKNIQQSIINMLNKKSGKNFKIDSPISIKLINERLKEGYVLEDFYKVIEVKVAKWK
                     NTIMEMYIRPETLFSYKFESYVNENISMKSQSNVHPCKEGNSNDSSNYIHSDYTTYDG
                     SVRERKKYENVRYWNPICEI"
BASE COUNT         1925 a          492 c          700 g         1849 t
ORIGIN      
        1 ttatactttt tatgtagtcc atatttaaaa tcatggattt agatattcgt gagaaggatg
       61 tattagctaa taattttgat aatttatata tttttaaatc aatttcaaat acatcatctt
      121 cataataagc aaatgtttta tttccaactg tctcaaagta taaaatatca cagacattga
      181 gtttataaat ctctcctgat tttttacctt ttatatcaag taacggtgta ctcaataatg
      241 aaattatttt ttcaatgtta ttatctagag cagaacattt tatggttatt tcaatatcat
      301 cacaattaat accttgttcg acatgtattt tcattaatca tcacctcatt ttattactgt
      361 cattagaata gcattatatc ttattgagtc caactagatg cgttctaaat ttcaaattaa
      421 gcatagtgaa attcaaaaat aaaacagtga aattcaaatt tgaggtggta aaattcaaaa
      481 aaatactatg gatatttgga taaattatcc aaatatccta gtatttcaca tagaatatgt
      541 ctaattattt tacaaataga cctattttat gacttacata tattgtcaaa ttacttctta
      601 aatatcaaaa tatttttgga taatgtatca atactgtttg ataaagtatt taatttaccc
      661 tcaattctaa ccaataagta catggataga gctataggaa aaccaacatt ggttattaac
      721 agttgtaaac tatcatccat aaaattagcc ccctattcta taactaagtc ataaggagtg
      781 gtatttgttt ccacaacctt ggcttcaaca acagaagcta ggtcaaatcc tccaaccgaa
      841 aaaatatttt tagatactat caaatccatg acagatttta tattttcttc agttaaatca
      901 tcctttggat tttctataga aaaagaaact tgtttatcct ttacattttt aaagctcatg
      961 actaattttt tcttaatttc catataatta cctccgatat aataatttat attttaattg
     1021 tttgcttgtt aatattggta ttttaattgt caatattaat gttttgcttg ttagtattaa
     1081 tatttcaatt gtcaatatta atgttttgct tgttagtatt aatatttcaa ttgtcaatat
     1141 tagtgtcttg cttgttagta ttaatatttc aattgtcaat attaatgttt tgcttgttag
     1201 tatcagtgtt tcaattgtca atattagtgt tttgcttgtt agtattaata tttcaattgt
     1261 caatattagt gtcttgcttg ttagtattag tatttcaatt gtcaatatta gtgttttgct
     1321 tgttagtatt agtatttcaa ttgtcaatat tagtgttttg cttgttagta ttagtatttc
     1381 aattgtcaat attaatgttt tgcttgttag tattagtatt tcaattgtca atattagtgt
     1441 tttgcttgtt agtattaata tttcaattgt caatattagt gttttgcttg ttagtatcag
     1501 tgtttcaatt gtcaatatta gtattttaat tgttgactct agttattgct gatatagaaa
     1561 atatttaatt tatttcaatt aattagatta aagtagggta gcatctaaaa tagctaacat
     1621 gtttaagttg aaatacgttt aatatattaa tttttcaaag cattaatata ttaaagttat
     1681 aatcaaaatt gaagttataa tctgccaaat ctatagcgta ttaagtgcgt tatataactt
     1741 aatcttttat ataaagagtt aattttttta atttgtaaag cagagtacgt ttaaaaggta
     1801 ctatttagat aatgatgtac tatctaattt taaaatatct acaatagggt tctcttgaag
     1861 acttgctatt gcttctgcaa cagcataaac atcttcattt aatgcctctg cttttacatt
     1921 tgcaaatgat ttacttttaa caactgcctt acccttatca tcaaaaccag catccaattt
     1981 tagttttata ctagataaat tttttgttga aacaacttcc ataaaattac ctcctaaaaa
     2041 tttaatttca aagatttgtc gactttcttt gttagctata atactatcat ctaagataat
     2101 atcggagtta aacagtaatt tctaaagatt ttttgtgtaa atttttatag gaaaaatttt
     2161 acttttttgc tgatgaaaaa gtttacaagt tatttgtgaa gatatgttct tagtgttttg
     2221 aaatagtatt tgtatatatt catgcttagt tgagatagtt ttgtatggat tttcatatgt
     2281 gtagagttaa atgttaggta ctaaagttta tgttaaatta aatgttaaag cttatgttaa
     2341 aaattttatt aaatttaatc tgtaaataaa aaagttaaaa gtccaatatt aacattttac
     2401 ttttaaacca ttaaaatctt tattaaagct aacaaagtta taagttatga gaaattttat
     2461 taaagttaat acaaaaatta attttaaagc taagaaattt tttattagat tcaataaaac
     2521 taataaatat agtttagtca ataatgtatt aaataaaaac tttgttaagt ctataaaaat
     2581 atcttaaagg aggtgtaact tatgaagata aaagtggagg atataccagt tcaatttcat
     2641 gcaatggtag aaataattgg aattgataag tttatagagg ttgccaagtt gtatggagga
     2701 acaaatactt atatacctac gtataagggg ctttttagat atgctagaaa tagggatata
     2761 gtaaaacaat ttaatggagt caaccacaat gaattggcta ttagatataa tatgtgtgta
     2821 agcaatatta agcgaatact caatgaaaat aatgtttaaa tataataaaa taatgtttaa
     2881 acataatgaa aatagccttg ttaaacgtaa gagtaaagac aaagaagatg ttttataaag
     2941 tttttttaga caaattaact aaatttacta cttattaatt taaatacatt gtatattcta
     3001 taaaaaaatt tgttaaatac tggagatata ttgagaatac aaagaaaatg gtcgaaaata
     3061 tttgttggct aaaaaataga taaaatgata caattgcttg tataaagggg aaaaataaat
     3121 aaataaacta tataaataaa gaaatagcaa ttaatatgac ataatatatt tataaaatta
     3181 agaatataaa tagatatttt aaggtattag ttgttaaatt aggaaatatt aaaaaaaata
     3241 tatatttata tttaaactat tttgttattt atataaaaat acttatctag ttactagcaa
     3301 ttattagtag aaatggggga aatagagatg aataagggat tttacaaatt aaattcagtg
     3361 gtattgaact ttgatattaa gaatgatgtt aacaagataa gatttaatca ttatataatg
     3421 agaaaagaaa acatacaaag acttcaagga actctgccaa acggacaatt ttatatgaca
     3481 gtgagaagag ctgctacaga tttacaactt tctatttcta ctataagtag gcttgtaaaa
     3541 gagtttttgg atttagggat aataagatta gtgtctaagg gtgtaaaagg taatcgttct
     3601 gtatatagtt acgtaagtat agagtttaat ggaagtttat ttgatgatag caaatatatg
     3661 gaacttgact gtgtatgtaa tgatgaattt attgataata gtggcataga gtttgattgt
     3721 gatagcaaag ataattcttt taatggagat ataaatgtag aaactgtgca ttgcattaat
     3781 gagtatttgt ctgatgatga tgtagagcat aattataaca atttccataa taaagatact
     3841 ataaaggata aagccatttc taatattagc actatcaaaa aatatattga tgaatctttt
     3901 gataaaaata aatcagaaaa ttctaactta caacaggagt caataaattt caactttaat
     3961 gatacatgca atgaaataga taaaagaacg aataatagta tagaaaataa tcaaaataaa
     4021 aggcctatga gattgagtga catcagtctt ttaagtgata aaggtatgac aaataaaaag
     4081 aattacttta atcataatca tggtatacaa aaagggacta aatctgtaac aaagaaaaaa
     4141 gaatccttaa ataaatttaa taaaaaaaat atacagcaaa gcattataaa tatgctaaat
     4201 aaaaaatcag gtaagaattt taaaatcgat tctccaataa gcataaaact tatcaatgaa
     4261 aggctaaagg aaggctatgt cttagaagat ttctacaaag ttattgaggt aaaagtggct
     4321 aagtggaaaa ataccataat ggaaatgtat ataagaccag aaactttgtt tagctataag
     4381 tttgaatcct atgtaaatga aaacatatct atgaaaagtc aaagtaatgt tcacccatgt
     4441 aaagaaggca atagtaatga tagcagtaat tatatacatt ctgattatac aacatatgat
     4501 ggcagtgtgc gtgagagaaa aaaatatgag aatgtaagat actggaatcc tatatgtgaa
     4561 atttaattag tttaagaatg tatttatgat aaataagata ttaatagaga atagtgagct
     4621 atagcaaatg atatatcaat agttggtagt aagttatatt aagcaagata ttactatcag
     4681 gtagtgaatt atattgaacg atatatcaat aattggtagt aaattatatt aagtaagata
     4741 ttactatcag gtagtgaatt atattgaacg atatatcaat aattggcagt aagttatatc
     4801 aaataagata ttaatatcga gtaatgaatt atattgaacg atatattaat aattggcagt
     4861 aagttatatc aaataagata ttactatcaa gtagtgaatt atattgaacg atatattaat
     4921 aattggaagt aagttatatc aaataagata ttactatcaa gtagtg
//
LOCUS       DAEPIN010000145         4840 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.147, whole genome shotgun sequence.
ACCESSION   DAEPIN010000145 DAEPIN010000000
VERSION     DAEPIN010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4840
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.147"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            226..1110
                     /locus_tag="KR250_003678"
     CDS             226..1110
                     /locus_tag="KR250_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HBH0069557.1"
                     /translation="MDTTKIDDISKLDLNNLDINKMDMGGIMESLLEWATTSGVKLII
                     GILILSIGFKIIKKFVNHVMLLLSKRDIDLTLRRFLKSLLLSVLKVAVIIIVLEYWGM
                     SLSSFAAVIASAGVAIGLALQGSLSNFAGGFIILLIRPFKVGDYVEAAGHGGTVEQIG
                     LFYTQLVTPDNKQILIPNGSVSNDSLINYSAKNTRRVDLTFSVGYEDDILHVRRVLKD
                     IVNNHKLIINEPEPFIGVVEHGDNAIKFATRVWCKTEDYWTIYLDLLEEVKVRFDEEG
                     ITIPYPKMDLTVKELNKI"
     gene            1469..2086
                     /locus_tag="KR250_003679"
     CDS             1469..2086
                     /locus_tag="KR250_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HBH0069558.1"
                     /translation="MCKKMYLLVVGILIAGLLTACNGSPTKDSEQAQDNTKKEANVSN
                     NALKDEKNNENLMEQDFKVPYTDAINIFKDKYKDADIVDLSLERDLNKFVYTVEGVDD
                     NNEYKMKIDANTKDVLEDKTEKLDSEDLNGVARKEKLDFNDIMTPQQAMEIALKEQNG
                     MVKEWSLDKDLDVTFYKIRIDKDKNEYDIKVDSKKGTVLKVEKED"
     gene            2194..4725
                     /locus_tag="KR250_003680"
     CDS             2194..4725
                     /locus_tag="KR250_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBH0069559.1"
                     /translation="MSLKSLKIKENLYWVGSLDPDLRVFDIIMYTPYGTTYNSYVLKG
                     TEKTVLFETVKDKHFDNYIERLNDLNIDFEKIDYIVVSHTEPDHAGSVEKLLDLAKNA
                     KVVASETAIKYLKEIVNKDFEYVAVTDGDTLSIGDKTLEFFSVPMLHWPDTIYTYIKE
                     DKTLVTCDSFGSHYSNDKIVNTLDEDEEKDYLDALRYYYDCIMGPFKPSMVTAIEKIK
                     DLDIDTVCPGHGPVLTENPRKIIDLYYNWSVNEQIKLEKEVTICYVSAHGYTKIMAEA
                     IKAYIEKNSNYKVNLFDVIEHKQEDILAKIAVSQGVLFGTPTILGDALKPIWDILISL
                     NPVLHGGKVASVFGSYGWSGEGIENAMERISQLRMTAVKPFAVNFKPSNEEIDKLHSY
                     TGKFLDKLNSTFGSKKKTKKFKCVICNEVFEGDSAPSVCPVCGAKEDQFIEVEEDEVT
                     FRKDTDEYFVIVGNGAAGFYAADAIRKRNKTCKITMISNEDELTYYRPALSDGINEEL
                     GSDFYMEDKDWYDKNNIVVILGTNVDKLDEVNKTIIVNDGAIKFDKLVIATGSRNFIP
                     PIKGHDLENVFTLRNIKDLYSVKEALEKSKKVVVIGGGLLGLEAAWEFRLKGLEVVVV
                     EAMDSILSKQLDKEGSKILEQCVRDTGIDVRLGVAVDGIEGDGKAQKVVFKDGDSVDC
                     DMVVFSIGVRANTQMVQDTSVKIDRGIVVDKTLQTNVKDIYACGDVAQVGNISLAIWP
                     SSVEMGKIAGANASGDNLTFESEVYPVSLDAMNVKVFSIGNIQNFDKEISSKDEGQRI
                     YKKLFMKDGSLVGAILINDLSCTVKLIRLISEKGDFEDIMKADIL"
BASE COUNT         1923 a          436 c          919 g         1562 t
ORIGIN      
        1 ggggtaaaaa atgatgattt taaggctata tagtgtttta ttaatagttt aatttagtat
       61 tatattttgc atatatatat aacaaatagt ttgcattttc ctaagatgta taattggaat
      121 ataaatcttg tttactatgt acaaatggta ttgtattata ttaaatagaa aataagaact
      181 ttaaactata atattggttc taaaatataa ggtaaggagt tttttatgga tacgacgaaa
      241 atagatgata taagcaagtt agatttaaat aatttagata ttaataagat ggatatggga
      301 ggtataatgg aaagtctatt agaatgggct actacaagcg gagtaaaact tataataggc
      361 atacttatac tttctattgg atttaaaata ataaaaaaat ttgtaaacca tgttatgtta
      421 ttacttagta aaagagatat tgatttaact ttaagaaggt ttttgaagtc tcttcttttg
      481 agtgtactta aagttgccgt tataatcatt gttctggaat attggggaat gagtttgtcc
      541 agttttgctg ctgtgatagc atcagcaggg gtagcaatag gtttggcttt acaaggtagt
      601 ctatcaaatt ttgctggagg atttattata ctattgatta gaccttttaa agtaggagat
      661 tacgtagaag cagcaggcca tggaggaacg gttgaacaaa taggactttt ttatacacaa
      721 ttggttactc cagataataa acagatactt atacctaatg gttctgtatc aaatgatagt
      781 ctaataaact attcagcaaa gaatactaga cgagttgatt tgacttttag tgtagggtat
      841 gaagatgata tactacatgt tagaagagta cttaaagata ttgttaataa tcataaatta
      901 ataataaatg aaccagaacc atttatagga gtagtagaac atggagataa tgctattaaa
      961 tttgcaacta gagtttggtg caagacagaa gattattgga caatttatct tgatttacta
     1021 gaagaggtta aagttaggtt tgacgaagaa ggaattacga ttccatatcc taaaatggat
     1081 ttaacggtaa aagaattaaa taaaatttaa tttttttatg tagtgtttaa atttaagaat
     1141 attatataga tatatttata agatttgtga aaaattaata agtcctgtta ttttgtaagt
     1201 gaaaatataa aaaatgggag tgtctcaaaa tgaacttttt agttcatgag gcactctttt
     1261 tgtatgaaat caaatatatt tttattattt caacaataaa aaagaggtct gtttctattt
     1321 tgagacaata ttatttttat gtagatttaa tcttaatgag tataaagttt taaaactact
     1381 aaaataggag gaattttaat ataaaagggt aaataaaggg tttagagggc ataatataga
     1441 ataaattaat tatttaagga gggtttttat gtgcaaaaaa atgtatttat tagtggtagg
     1501 tatacttata gcaggtttat taactgcatg taatgggagt ccaactaagg attcagaaca
     1561 agcacaagat aatacaaaaa aggaggcaaa tgtatcaaat aacgctttaa aagatgaaaa
     1621 aaataatgaa aatttaatgg aacaagattt taaagtgcca tacacagatg caataaacat
     1681 atttaaagac aaatacaaag atgctgatat agtagattta agtcttgaga gagatttaaa
     1741 taaatttgtt tatactgtgg aaggtgtaga tgataataat gaatataaga tgaaaattga
     1801 tgctaataca aaagatgtat tagaagataa aacagaaaag ttggattcag aagatttaaa
     1861 tggagtcgct agaaaagaaa aattagattt taatgacata atgacacctc aacaagctat
     1921 ggaaattgca ttaaaagaac aaaatggaat ggttaaagag tggagtttag ataaagattt
     1981 agatgttact ttttataaaa taagaataga taaagataaa aatgaatatg acataaaagt
     2041 agattctaaa aagggaactg tattgaaggt agaaaaagaa gattagaaga tttaaactat
     2101 ctttaaaata atttcttttt tgaataaaat ttgtttaaaa tataacaagt agggtataaa
     2161 gagactaaat aactatttta ggaggagtac ggtatgtcat tgaaatcgct aaaaataaaa
     2221 gaaaatttat attgggtagg ttcgcttgac cctgatttaa gagtatttga cataattatg
     2281 tatacacctt atggaactac ttataattca tatgtattaa aaggaacaga aaaaacagtt
     2341 ttatttgaga cagttaaaga taagcatttt gacaattata ttgaaagatt aaatgattta
     2401 aatatagatt ttgaaaaaat agactatatt gttgtaagtc atactgaacc agaccatgca
     2461 ggaagcgttg aaaagctttt ggatttagct aaaaatgcaa aagtagtagc ttcagaaaca
     2521 gcaattaaat atcttaagga aatcgtaaac aaagactttg aatatgttgc agttacagat
     2581 ggagatacat tatcaattgg ggacaaaact ttagaatttt tctctgtacc tatgcttcat
     2641 tggccagata ccatatacac atatataaaa gaggataaaa ctcttgtaac atgtgattca
     2701 tttggtagtc actatagtaa tgataagata gtaaatacac ttgatgaaga tgaagaaaag
     2761 gattacttgg atgctttgag atactattat gattgtataa tgggaccatt taaaccatct
     2821 atggtaactg ccatagaaaa gataaaagat ttagatatag atactgtatg tccaggtcat
     2881 ggtccagtat taactgaaaa tcctagaaaa attatagacc tttattacaa ctggagtgta
     2941 aatgaacaaa taaagttaga aaaagaagtt acaatttgtt atgtttctgc acatggatat
     3001 acaaagatta tggcagaagc tataaaagca tatattgaaa aaaatagcaa ctataaagta
     3061 aatttatttg atgtaataga gcataagcaa gaagatattt tggcaaaaat agcagtatca
     3121 caaggtgtat tatttggaac accaacaata ttaggagatg cgttaaagcc tatatgggat
     3181 atattaatat cgttaaaccc agttcttcat ggtggaaaag ttgcttcagt atttggttca
     3241 tatggatgga gtggagaagg tatagaaaat gctatggaga gaataagtca gcttagaatg
     3301 acagcagtaa aaccttttgc tgttaacttt aaaccatcaa atgaagagat tgataaatta
     3361 cattcttata caggtaaatt cctagataag ttaaattcta cttttggaag taaaaagaaa
     3421 actaagaaat ttaaatgtgt aatttgtaat gaagtttttg aaggtgatag tgctcctagt
     3481 gtatgccctg tatgtggagc taaggaagac caatttatag aagttgaaga agatgaagtt
     3541 acttttagaa aagatactga tgaatatttc gtaatagtag gaaatggagc agctggtttt
     3601 tatgcagcag atgctataag aaaaagaaat aaaacttgta agataactat gatttctaat
     3661 gaagatgagt tgacttacta tagacctgca ctttctgatg gtataaatga agaacttggt
     3721 tcagatttct acatggaaga taaagattgg tatgataaaa ataatatagt agtaatatta
     3781 ggaacgaatg tagataaatt agatgaagtg aataaaacta ttatagtgaa tgatggagct
     3841 attaaatttg ataagcttgt aatagctaca ggaagtagaa actttatacc accaattaag
     3901 ggtcatgatt tagaaaatgt gtttactctt agaaatataa aagatttata tagtgtaaaa
     3961 gaagcactag aaaaatctaa gaaagttgta gtgattggtg gcggtttatt gggacttgaa
     4021 gctgcatggg aatttagact aaaaggatta gaagtagtag tagttgaagc catggatagc
     4081 atactatcaa aacaacttga taaagaaggc agtaaaatat tagaacaatg tgtaagagat
     4141 acaggaatag atgtaagatt gggtgtagca gttgatggta ttgaaggtga tggaaaagct
     4201 caaaaagttg tatttaaaga tggagatagt gttgactgtg atatggtagt attttcaatt
     4261 ggtgtaagag caaacactca aatggttcag gatacatctg taaaaattga cagaggtatt
     4321 gttgtagata agacattaca gacaaatgtt aaagatatat atgcttgtgg agatgttgca
     4381 caagtgggaa atattagttt agcaatatgg ccatcttcag tagaaatggg aaaaatagca
     4441 ggggcaaatg caagtggaga taatttgaca tttgaaagtg aagtatatcc agtttcatta
     4501 gatgcaatga atgtaaaagt atttagtatt ggtaatatac aaaattttga taaagaaatt
     4561 tcatcaaaag atgaaggtca aagaatatat aaaaaattat ttatgaaaga tggttcatta
     4621 gttggagcta tacttataaa tgatttaagc tgtactgtta aattgataag attaatatct
     4681 gaaaaaggtg attttgagga tattatgaag gctgatatac tataatttaa atacattaaa
     4741 ataaaaagga gttgtcttaa aattgatttt tttctaatca aattttaaat tcaactcctt
     4801 ttttgaattt ttatataaaa taacatggaa attttagtaa
//
LOCUS       DAEPIN010000146         4833 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.148, whole genome shotgun sequence.
ACCESSION   DAEPIN010000146 DAEPIN010000000
VERSION     DAEPIN010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4833
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.148"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            complement(204..1388)
                     /locus_tag="KR250_003681"
     CDS             complement(204..1388)
                     /locus_tag="KR250_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBH0069560.1"
                     /translation="MLSKRLNFITPSYTIGISSKVKEMESNGVKVINLSIGEPDFNVP
                     NNAKSYGIDSLNKDYTKYDLVPGLKILREEICKKLIEENNCNYSIDEIVVSSGAKNSI
                     TNTLLALTDEGDEVLLPKPYWVSYPEMIKLVNAVPVFIDTKKENGFKLTKEELEKSIT
                     DKTKILVINNPSNPTGSVYTKDELIEIVDVCIQNKIYILADEIYEKICYTGEFTSIAS
                     LSEEAKDITITINGFSKSAAMTGLRLGYTASNKTIAKAMSSIQGHLISHPSLTAQYIA
                     YGALKDCSIDIDNMVKTYKSRRDLIKSKLDSIDNVGYVNPNGAFYIFIDLSKVSEKFE
                     YKDSFSIEFCNQFLEEYNVAVVPGIAFGMDKYIRISYACSENTFLSGLDKLKEFVYKI
                     MA"
     gene            1761..4112
                     /locus_tag="KR250_003682"
     CDS             1761..4112
                     /locus_tag="KR250_003682"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside triphosphate reductase"
                     /protein_id="HBH0069561.1"
                     /translation="MVEFVKKRDGRVIPFNEDRITRAIFLAATNVAEREGIVPDYKLS
                     EQLTQEVIKFLNHKYSESVPSVEDIQDSVVKVLIETGHAKTSEEYIIYRTERSRIRNS
                     KTRLMKAIEEITFEDAEDADIKRENANINGNTAMGTMLQYGSTVSKEFCKTHILKPEH
                     SFAHDNGDIHIHDMDFLNMGTLTCCQIDVKKLFNGGFSTGHGFLREPQDIISYGALAA
                     IAIQSNQNDQHGGQSIPFFDYGLAEGVYKTFKKFYIGNLAKALKLFKGIENNDVIKNI
                     VYNTEKETNQKVGLKRDELYLNLEKEKLIQTFDIDDELVNKMQNFAFEESYRETDKKT
                     YQSMEAFIHNLNTMHSRAGAQVPFSSVNFGTDTSEEGRMVTKNLLLSQERGLGNGETP
                     IFPILIFKVKEGINLNPEDPNYDLFKLSCRVSAKRLFPNFSFLDAPFNAKYYKKGEPD
                     TEATYMGCRTRVLSNVCGSETVSGRGNISFTTVNLPRLGIKHGIINNEKANLDGFFEE
                     LDEKINLIIEQLLERLEVQGNKKMKNFPFLMGQGVWKGSDDLGPEDTLKEVIKQGTLT
                     IGFIGLAECLIALIGKHHGESKEAQELGLKIVSHMRHKMDEATDKYKLNFSLMGTPAE
                     GLSGRFTKIDKKVYGEIKGITDKEYYTNSFHVPVYYNISAYDKIEIEAPYHELTNAGH
                     ITYVELDGDPSDNLEAFETVIKAMKDLGIGYGSINHPVDRDPICGFSGVITSNICPVC
                     GRNEDESDIKFERIRRITGYLVGTVDRFNNAKKAEVRDRVKHR"
     gene            4134..4673
                     /gene="nrdG"
                     /locus_tag="KR250_003683"
     CDS             4134..4673
                     /gene="nrdG"
                     /locus_tag="KR250_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HBH0069562.1"
                     /translation="MKIRMSSTISYDSIVDGPGLRMVIWTQGCIHNCKECHNPQTHDL
                     CGGFYMDTEEIINKVKSLKLQKGITLSGGEPFLQPEPLEEIAKEAKINGLDVWSYTGF
                     TFEQLLDKKNRAYFKNLNLLKQIDILVDGKFIAEKKDISLKFRGSSNQRIIDVQKSLK
                     YKQVFLVEQYMKDDLSIAE"
BASE COUNT         1864 a          563 c          779 g         1627 t
ORIGIN      
        1 ttataataaa tttagaattt aacttatata gtttaatttg ataatttgat gaaaaaaata
       61 aaagacttat tgatttttaa atctataggt cttttatttt ttatctaaca tttaaattaa
      121 gttgttgaat atatcttata ttacttacct tatatattaa ttatcattaa tacattatat
      181 ctaattacta ttcatctaaa ttattatgcc attattttat atacaaattc ttttaattta
      241 tctaatccag ataagaaagt attttcactg caagcatagg atatacgaat atatttatcc
      301 ataccaaatg ctatgccagg aacaactgca acattatatt cttctaaaaa ttgattacaa
      361 aattctatag aaaaactatc tttatattca aatttctcag atactttaga aagatctata
      421 aatatataga atgcgccatt tggattcaca tatcctacat tatcaataga gtctagtttt
      481 gatttaatta aatctcttct tgatttatat gttttaacca tattgtctat gtcaattgag
      541 caatctttca aggctccata ggctatgtat tgtgctgtta gacttgggtg agatataaga
      601 tgtccttgta tagaactcat tgctttagca atcgttttat tagatgcagt atatcctaat
      661 cttaatccag tcattgcagc tgacttagaa aaaccattta tagttattgt tatatcttta
      721 gcttcttcgc ttaatgatgc aattgatgta aattcaccag tgtagcatat tttttcatat
      781 atttcatctg ctaaaatata tattttattt tgtatgcata catctactat ttctattaat
      841 tcatcttttg tataaacact accagtagga tttgaaggat tgtttattac aagaattttt
      901 gttttatctg ttatagattt ttcaagttct tcttttgtta acttaaatcc attttctttt
      961 ttagtatcaa taaaaactgg aacagcatta actaacttaa tcatttcagg ataactaacc
     1021 caataaggct ttggtaataa gacttcatct ccttcgtctg ttaaagctag taaagtattt
     1081 gttattgagt tttttgctcc acttgataca actatttcgt ctattgaata gttacagtta
     1141 ttttcttcaa taagtttttt acagatttcc tctctaagta tttttaatcc tggaactaaa
     1201 tcatatttag tgtaatcttt atttaaagaa tcaataccat aagattttgc gttattaggt
     1261 acattaaaat cgggttctcc tatactaaga tttattactt taactccatt actttccatt
     1321 tctttaactt tagaactaat acctattgtg taggatggtg ttataaagtt tagtcttttt
     1381 gataacataa aatgcctcct tttaaattat tatttatatt atacgacaaa tttaaagaat
     1441 ttacataagg aaatttgtag atatatacat taatatatat aatagcctac actttttgcc
     1501 taaaattagt gtaaaaaaaa gagaaaaaaa ttttaacttt caaagttgtt agaaacgcta
     1561 ctgttcaaaa tatccacata gtatttgcaa tgatttgcgc aagtttgact attatgtaca
     1621 aatttggtaa tcttaatata gcaattatta agataatccc tttaacttag ttgaagggga
     1681 tttttttaga cttaacatac tacatatagt ggtatgggga ttaataaaat acaatatata
     1741 gtaacaggag gtttttttaa ttggttgaat tcgtaaaaaa aagagatgga agagtaattc
     1801 catttaatga agatagaata acaagagcta tatttttagc agcaacgaat gttgcagaaa
     1861 gagaaggaat agtacctgat tataaattat ctgagcaatt aactcaagaa gtaataaagt
     1921 ttttaaatca taaatactca gaatcagttc caagtgttga agatatacaa gattctgtag
     1981 ttaaggttct tatagaaact ggccatgcaa aaactagtga agaatatata atatacagaa
     2041 ctgaaagaag tagaataaga aattcaaaaa caaggttgat gaaggcaata gaggaaatta
     2101 cctttgaaga tgcagaagat gctgatataa aaagagaaaa tgcaaatatt aatggtaata
     2161 cagcaatggg aactatgtta caatatggaa gcactgtatc taaagaattt tgtaaaacac
     2221 atatattaaa accagagcat tcttttgctc atgataatgg agatatacac atacatgata
     2281 tggatttttt aaatatggga acattaactt gttgtcaaat agatgttaaa aaactattca
     2341 atggtggatt ttcaacagga catggctttt taagagagcc acaagatata ataagttatg
     2401 gtgcattagc tgcaattgct atacaaagca atcaaaacga tcaacatgga ggtcaaagta
     2461 taccattttt cgattatgga ttggctgaag gtgtatataa gacatttaag aaattctaca
     2521 taggaaattt agctaaagca ttaaagttat ttaaaggaat tgaaaataat gatgtgataa
     2581 agaacatagt atataataca gagaaagaaa caaatcaaaa agttggtctt aagagagatg
     2641 aattgtatct aaacttagaa aaagagaaat taatacagac ttttgatata gatgatgaac
     2701 ttgtaaataa gatgcaaaac tttgcatttg aagaatcgta tagagaaact gataaaaaaa
     2761 catatcaatc tatggaagca tttatacaca atctaaacac aatgcactct agagcaggtg
     2821 cacaggtacc attttcaagt gtaaacttcg gaacagatac ttctgaagaa ggaagaatgg
     2881 taactaagaa tcttctatta tctcaagaaa gaggtttagg taatggagag acaccaattt
     2941 ttccaatttt aatatttaag gtcaaagaag gcataaattt aaatcctgaa gaccctaatt
     3001 atgatttatt taaattatca tgcagagtat cagctaaaag actattccct aattttagtt
     3061 ttttagatgc gccttttaat gcaaaatatt ataaaaaagg tgaaccagat acagaagcta
     3121 catatatggg ttgtagaaca agagtactta gtaatgtgtg tggttcagaa acagttagtg
     3181 gtagaggaaa catatctttt actacagtaa atttaccaag attgggtata aaacatggta
     3241 ttataaataa tgaaaaggct aatttagacg gtttctttga agaattagat gagaagataa
     3301 acttaattat agaacaatta ttggagcgcc ttgaggtaca aggaaataaa aagatgaaga
     3361 attttccatt cttaatgggg caaggtgtat ggaaaggttc tgatgattta ggaccagaag
     3421 atacactaaa agaagtaata aaacaaggaa cattgacaat tggatttata ggtttagcag
     3481 agtgccttat tgctcttata ggtaaacatc atggagaaag taaagaagct caagaattag
     3541 gattaaaaat agtgtcacat atgagacata aaatggatga agcaacggac aaatataaat
     3601 taaatttctc cttaatgggt actccagcag aaggtctttc aggtaggttt actaaaatag
     3661 ataaaaaagt ttatggagaa attaaaggta tcacagataa agaatattat actaattcat
     3721 tccatgtacc agtttattac aatataagtg catatgataa aattgagata gaagctccat
     3781 accatgaatt gactaatgca ggtcatataa catatgtaga gttagatgga gatccatctg
     3841 ataatttaga agcttttgaa actgttataa aagctatgaa agatttgggt ataggatatg
     3901 gaagtataaa tcatcctgta gatagagacc caatatgcgg tttttcaggt gtaataacaa
     3961 gtaatatatg ccctgtatgt ggaagaaatg aagatgaaag tgatatcaaa ttcgaaagaa
     4021 taagaagaat aacaggatat ttagttggta cagttgatag atttaataat gctaaaaaag
     4081 cagaagtaag agatagagta aaacatagat agttttattt gggggaataa gttatgaaaa
     4141 taagaatgtc atctactata agttatgaca gcatagttga tggaccagga ttaagaatgg
     4201 taatatggac ccaaggatgt attcataatt gtaaagagtg ccataatcct cagacacatg
     4261 atttatgtgg aggattttat atggatacag aagaaattat aaataaagtc aaatcattga
     4321 aattacaaaa aggcattacg ctatcaggag gagaaccatt tttacaacca gaaccattag
     4381 aagaaatagc aaaagaagct aaaataaatg ggttagatgt atggtcatat actggattta
     4441 cgtttgaaca actgttagat aaaaaaaata gagcgtattt taaaaattta aatttactaa
     4501 agcaaattga tattttagtg gatggaaaat ttatagctga aaaaaaagat ataagcttaa
     4561 agtttagagg gtcatcaaat caaaggataa ttgatgttca aaaaagtttg aaatataaac
     4621 aagtgttttt ggttgagcaa tatatgaagg atgatttatc tatagcagaa taaaatataa
     4681 ataaacagga ttaaatatat gcatatattt aatcctgttt attttcatat aatatttcaa
     4741 gcttgtataa tgtaacaatt aaaccaaaag actcattgta tatttgaaat taagtttttt
     4801 atagtttata ggtgttttat gtggattacg aat
//
LOCUS       DAEPIN010000147         4780 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.149, whole genome shotgun sequence.
ACCESSION   DAEPIN010000147 DAEPIN010000000
VERSION     DAEPIN010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4780
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.149"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            complement(<1..402)
                     /locus_tag="KR250_003684"
     CDS             complement(<1..402)
                     /locus_tag="KR250_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HBH0069563.1"
                     /translation="MMFDRVKWNKLDYNELLEYMMSIQDIKYRDFNKKLIPGTENIIG
                     IRVPNLRKLSKEISQGNWKEFLEVAEDTYYEEVRLQGMVIGNINSNFEETLYYVKRFI
                     PKIDNWSVCDGFCSDLKSMKKYKENMYDILKK"
     gene            complement(529..1218)
                     /locus_tag="KR250_003685"
     CDS             complement(529..1218)
                     /locus_tag="KR250_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBH0069564.1"
                     /translation="MDRYSEITNKNQREIVLLKGFPCIWGKCSFCDYIDDNSNLEEEM
                     NKLNLKVLKNVTGKYGVLEVINSGSCFELPKDTLEKIKCIIKEKNIKKLFLESHWSYK
                     NRLKEMREYFEIPVVFKIGVETFDNDFRNNILNKNANFKTPQDVKEYFDSPCIMVGIK
                     GQTKEMIDKDIEIILNTFEKATVNVFINNSSSIKRDEELVKWFVSKYKFLDENPNIEV
                     LYNNTDFGVGD"
     gene            complement(1226..1789)
                     /locus_tag="KR250_003686"
     CDS             complement(1226..1789)
                     /locus_tag="KR250_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBH0069565.1"
                     /translation="MKRKICVKTITLIAFGISINIIGAFIAMGLRLPVYLDSIGTIMI
                     ASLLGPKYAVVTGVFGSLISGITFDVYSLYFAPVQISTGLLAGIAFKKNFLRGLKTPL
                     GVLLFAIPTSIISSIISAFLFGGMTSSGSSYIVQILKVLGLGDVFSVFVTQVFTDYGD
                     KLLAVVLVNLGLNAVPKTLKVSLTGGK"
     gene            complement(1791..2759)
                     /locus_tag="KR250_003687"
     CDS             complement(1791..2759)
                     /locus_tag="KR250_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HBH0069566.1"
                     /translation="MEKRKVIIDCDPGIDDSLAILLALNSPELEVIGITTCCGNVPAN
                     IGAENALKTLQMCSSLNIPVYIGEEAPLKRKLVTAQDTHGEDGIGENFYQKVVGAKAK
                     NGAVDFIINTLYNHEKVSIIALAPLTNIAKALIKDKKAFENLDEFVSMGGAFRIHGNC
                     SPVAEFNYWVDPHGADYVYKNLSKKIHMVGLDVTRKIVLTPNIIEFINRLDKKMAKYI
                     TEITRFYIDFHWEQEGIIGCVINDPLAVAYFIDRSICKGFESYVEVVEDGIAMGQSIV
                     DSFNFYKKNPNAIVLNEVDEKKFMYMFLKRLFKGYEDIIDSVEGVI"
     regulatory      complement(2860..2903)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            3268..3897
                     /locus_tag="KR250_003688"
     CDS             3268..3897
                     /locus_tag="KR250_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBH0069567.1"
                     /translation="MSIKERRKNEKEEMKKKIMDASIEIINQHGYENLSIRKIATKIE
                     YSPTTIYLYYKDKAEIISDMTNKLYNTVESNAIDIMNNCSSLPIDKQIIEIMTSFIKT
                     LSSEPEMAKSIMYSRMNIIFASENTNNTPSNNGIKMLDKFLSIGIEQGIFKKNIDKSS
                     WMLISALLGFVLCVVENKLYSLHNFSQLIENFLDILVGGLYNENSQQGY"
     gene            3872..4276
                     /locus_tag="KR250_003689"
     CDS             3872..4276
                     /locus_tag="KR250_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HBH0069568.1"
                     /translation="MKIRNKATNKKKKYVKISIALFFVFICIIIVIVSNLKPENLVVK
                     DIDINNVKNGIYTGSADNNLVKATVSVEVNNGKIQNINILKHDHLLGKPAEKITTSII
                     KQQSLDVDAITSATYSSNTIRKAVENALRKGE"
     gene            4279..4752
                     /locus_tag="KR250_003690"
     CDS             4279..4752
                     /locus_tag="KR250_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HBH0069569.1"
                     /translation="MNTIIIYSSKYGCTKDCANILKNKLSDNVTIVDINNNNKIELSK
                     FDKIIIGSSIYVGSVSKKIQVLCNDNVELLNKKQVGIFLCCGFSEQADKYLKSNFPSS
                     LLESANAIGIFGSEARLEKMKFLDKLIMKAVSKGNYDSFRISQDNIDNFLINLNS"
BASE COUNT         1734 a          752 c          538 g         1756 t
ORIGIN      
        1 ttttttaagt atatcataca tattttcttt atattttttc atagacttta aatcagaaca
       61 aaatccatcg caaactgacc aattatcgat tttaggtata aatcttttta catagtataa
      121 agtttcttca aaattactat ttatattgcc aattaccata ccttgtagcc taacttcttc
      181 atagtatgta tcctctgcta cttctaaaaa ttccttccaa ttcccttggc ttatttcttt
      241 tgataacttt cttaaattag gaactctaat acctattata ttttctgttc caggtataag
      301 ctttttatta aaatctctat attttatatc ttgtatagac atcatatatt ctaaaagttc
      361 attataatcc aatttattcc attttaccct atcaaacatc atttaattgc tccttaattc
      421 cttttttctt attgtaacat aaaactctac aaaaaaagct tctaactatt agaagctttt
      481 tcaaaaacat aagaaacttt tcaattttaa atttaaacta aattaatgct aatctcctac
      541 accaaaatct gtattattgt ataaaacttc tatatttgga ttttcatcca agaatttata
      601 cttactaaca aaccatttca ctaactcttc atctcttttt atactagaag aattatttat
      661 aaaaacattc actgtcgctt tttcaaaagt atttaggata atctctatat ctttatctat
      721 catctctttt gtttgtcctt ttattccaac cataatacaa ggtgaatcaa aatattcttt
      781 tacatcttgt ggagtcttaa aatttgcatt tttatttaaa atattatttc taaaatcatt
      841 gtcaaaggtt tcaactccaa ttttaaatac cacaggaatt tcaaaatact ctctcatttc
      901 cttaagccta tttttgtaag accaatgact ttctaaaaat aattttttta tatttttctc
      961 ttttataata cactttattt tttctaaagt atccttaggt aattcaaaac agcttccaga
     1021 atttatgact tcaagaactc catatttccc agttacgttt tttaaaacct ttagatttaa
     1081 tttattcatc tcttcttcta aattagaatt atcatctata taatcacaaa aagaacactt
     1141 tccccatata caaggaaatc ctttaagcag aacaatttct ctttgatttt tatttgttat
     1201 ctcactatat ctatccattt tctatctatt ttcctccagt caatgatacc tttaaagttt
     1261 ttggaactgc atttaatcct aaattcacta aaacaactgc taacagctta tctccataat
     1321 cagtaaatac ttgggttaca aacacactaa aaacatcccc cagtcctaac actttcaaga
     1381 tttgcactat atatgatgaa cctgaagagg tcatacctcc aaataaaaat gctgaaatta
     1441 ttgaacttat tatagatgtt ggaatggcaa ataaaagtac tcctagtgga gtttttaaac
     1501 cccttaaaaa attcttttta aatgctattc cagcaagtaa tccggttgat atttgaactg
     1561 gtgcaaaata gagagaatac acatcaaacg taattccact tattaaactt ccaaacacac
     1621 ctgtaacaac agcgtattta ggtcctaaaa gtgaagctat cattatagtt cctattgaat
     1681 ctaagtatac aggtaatctt aatcccatag ctataaaagc gcctattata tttatagaaa
     1741 taccaaaagc tataagtgta atagttttta cacatatttt tcttttcaca ctatatcact
     1801 ccttccacag agtctataat gtcttcataa cctttaaaaa gcctctttaa aaacatgtac
     1861 ataaatttct tctcatcaac ttcatttaga acaattgcat taggattttt tttatagaaa
     1921 ttgaaagaat ccactataga ctgacccata gctattccat cttctacaac ttctacatat
     1981 gattcaaatc ctttacatat acttctatct ataaagtacg ctactgctag agggtcattt
     2041 atcacacagc caattattcc ctcctgttcc caatggaaat caatataaaa tctagttatt
     2101 tcagttatat actttgccat cttcttatca agtctattta taaactcaat aatattagga
     2161 gtaagtacaa tttttctagt tacatctaaa cctaccatgt ggattttttt agataaattc
     2221 ttgtaaacat aatctgctcc atgtgggtct acccaataat taaactctgc tactggagag
     2281 caatttccat gaatcctaaa tgctcctccc atagatacaa actcatcgag attttcaaat
     2341 gctttcttat ctttaataag tgctttagct atatttgtaa gtggtgcaag tgctattatt
     2401 gatacttttt catgattata taaagtattt attataaaat ccactgctcc attttttgct
     2461 ttagctccta caaccttttg ataaaagttt tctccaatac catcttctcc atgtgtatct
     2521 tgagctgtta caagttttct ttttagtggt gcttcttctc ctatatatac tggaatattt
     2581 agtgaagaac acatttgaag tgtttttagt gcattttctg cacctatatt tgctggaaca
     2641 tttccacaac atgtggtaat tccaattact tctagctctg gtgagtttaa agccagaaga
     2701 attgccaaag aatcatcaat tcctgggtca caatcaatta ttacttttct cttttccatt
     2761 ttttatcctc cttgatttgt ggtcgaggag aactacacat cttttttcca ctttcaaaaa
     2821 ggtgtgtcta agggtattaa ttatatactg ccactatata attccttagt tttttatact
     2881 tggagttctc gaacaagtac agatgtaaaa tcatattttt aggctactta ctatagactc
     2941 ttaaaaacta tagactgtta aaaaattttt aaacatctga tttaatagct ataaaataaa
     3001 ttttatagca taaaagaatt tattatattt taatgtatct tattaaagtt tatttagaat
     3061 tatgaaccaa ctttaataag atgtggcgca tttaaaccga ttattattat acaataatat
     3121 cttaaatatt acaattaata acattaattt attatccata aaaatatatt aacttatatt
     3181 atttaataat ttattgacag tattaaaatt aaattataca ataacagtgt aaattaactt
     3241 aacaccgtta agcaaagagg tgattatatg agtatcaaag aaagacgtaa aaatgaaaaa
     3301 gaagaaatga agaaaaagat tatggatgct agcatcgaaa ttataaacca acatggatat
     3361 gaaaatttat caataagaaa aattgctaca aaaatagaat attctccaac tacaatttat
     3421 ctttactaca aagacaaagc agagattatc agcgatatga ccaataaact ttataacaca
     3481 gtagaaagta atgccattga cattatgaat aattgctcat cactaccaat agataaacaa
     3541 atcatagaaa ttatgactag ctttataaaa actctttcta gtgaaccaga gatggcaaaa
     3601 tctatcatgt atagtagaat gaatattata tttgcatctg aaaatacaaa caatacgcct
     3661 agcaataatg gtataaaaat gcttgataaa ttcctttcca ttggaattga acaaggaata
     3721 ttcaaaaaaa atatagataa aagctcatgg atgcttataa gtgcattgtt aggattcgtt
     3781 ttatgtgttg ttgaaaataa actatattct ttacataatt tttctcaact tatagaaaat
     3841 ttcttagata ttttagtggg aggactttac aatgaaaatt cgcaacaagg ctactaataa
     3901 aaagaaaaaa tatgtaaaaa tatctattgc tctattcttt gtatttatat gcattattat
     3961 tgtaattgta agcaacttaa agccagaaaa cttggtagta aaagatatag atattaataa
     4021 tgttaaaaat ggcatttata ctggaagtgc tgataataac ttggtaaaag ctactgtgtc
     4081 tgttgaagtt aataatggta aaatccaaaa tattaatatc ttaaaacacg accatctttt
     4141 aggtaaacct gctgaaaaaa ttacaacaag tattattaaa caacagtctt tagatgtgga
     4201 tgctataact tctgcaactt atagcagtaa tactatacga aaagctgtag aaaatgcttt
     4261 aagaaaggga gaataattat gaatactatt attatatatt caagtaaata tggttgtaca
     4321 aaagactgtg caaatatact aaaaaataaa ctatcagata atgtcactat tgttgatatt
     4381 aataataata ataaaattga gttatcaaaa tttgataaaa ttattattgg aagttctata
     4441 tatgttggtt cagtttcaaa aaaaatacaa gtgctatgta atgataatgt agagttatta
     4501 aacaaaaaac aagttggcat tttcctttgc tgtggctttt ctgaacaagc agataaatat
     4561 ttaaaatcaa attttccatc atccttatta gaaagtgcaa atgctattgg aatttttggt
     4621 agtgaagctc gattagaaaa aatgaaattt ttagataaac ttattatgaa ggctgtttca
     4681 aaaggtaatt atgacagttt tagaatatca caggataata tagataattt tttaataaat
     4741 cttaatagct aaaataaaac taattatttc tatgataaat
//
LOCUS       DAEPIN010000148         4672 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.151, whole genome shotgun sequence.
ACCESSION   DAEPIN010000148 DAEPIN010000000
VERSION     DAEPIN010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4672
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.151"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            <1..1254
                     /locus_tag="KR250_003691"
     CDS             <1..1254
                     /locus_tag="KR250_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HBH0069570.1"
                     /translation="TIDKFVIDANKILCKNTQVKYALLYIDIDKFKYINDLFGYEVGN
                     EILRNLTKIIKRNIFEEEMFARISADNFIIIMKYIEEEDITKRLKTIFEELDLFNNNQ
                     EEKYKLVLSCGIYFILPEDRDINSIIDRANIPHKMAKGGHKSSYAFYDNKIHDQEIKE
                     KEMENTMFSSLENKEFIIYLQPKIELNTGEIQGSEALVRWKRPDKGLIPPNEFIPFFE
                     RNGFVINLDLYVLEEVCIYLRKWIDAGINPVTVSVNVSRIHLYCNNFIETYKNIIDKY
                     NIPAKYIELELTESIIFDNFDILIDIMNNLKKIGFLISMDDFGSGYSSLNMLKEIPMD
                     ILKLDQKFIMETYNSKRSKIIVTKVIEMAKELGMKVISEGVETEEQFKLLKEVKCDMA
                     QGYLFGKPMPIEEFEHLIVSNLVRG"
     gene            1430..1798
                     /locus_tag="KR250_003692"
     CDS             1430..1798
                     /locus_tag="KR250_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBH0069571.1"
                     /translation="MEWELDNNKPIYIQLVEHLKLKIISGEIKIGSKLETVRALAEDA
                     EVNPNTMQKALTELERQGLVYSQRTKGRFVTDDKEKIKAMKEEIANVEINTLKVTLEK
                     LGYDRDEMLKLITENLKGEL"
     gene            1800..2525
                     /locus_tag="KR250_003693"
     CDS             1800..2525
                     /locus_tag="KR250_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0069572.1"
                     /translation="MDKNISNVSQDGIVEFKGVNKSYGTKNVLKNIDLNIPKGKIVGL
                     LGPNGSGKSTMIKLMNGLLQPDNGEIMINGMKPSIDTKKIVSYLPERTYLNDWMKVSD
                     LLKFFYDFYSDFDVRRANEMIKSLDIDVNEKLKTMSKGTKEKVQLILVMSRNASIYIL
                     DEPIGGVDPAARSYILKTILKNYSEDSTLLIATHLISEIENICDEIIFISKGEIVLQG
                     DVEAIREEKGKSIDALFREEFKC"
     gene            2519..3283
                     /locus_tag="KR250_003694"
     CDS             2519..3283
                     /locus_tag="KR250_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0069573.1"
                     /translation="MLGKLLKYELKASGRIFIPLYIAILIVAVFNGIFMNTNILQVQG
                     IGILVLTSLFMALGVLTIVVTIQRFRKNLLGDEGYLMFTLPVSTSSLILSKCITALIY
                     AVLSFIVAVFTFGVLMLFGTSGILLPEILDLFNTSFKWISENFLDILLLVVVMFISYS
                     SFILLLYTSISMGQLPKFNKHRNIVAFASFIAINIVISIVGDAVGSILPNEDTNMVYH
                     LYQSPSFMLAILGSLVVAIALFFATKFILDKKLNLE"
     gene            3505..>4672
                     /locus_tag="KR250_003695"
     CDS             3505..>4672
                     /locus_tag="KR250_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBH0069574.1"
                     /translation="MKSYTKAKWSVWGIITFSFVLVLFLRMSTAVVSDNLANELGFNS
                     IQISNIASFCLYAYAFMQIPAGILIDKYGARKISSLGIIMASLGSILFGLIQSIELAY
                     ISRVIVGAGTSVILLCILKIQGRWFNKSEFASATAKFSFVGNLGGVLATFPLVFLSEL
                     VGWRNSFLLIGIIGVVIGCFMYIIVRDTPKEYGFNIDTEPYEKSEKVNIVDGIKSVIK
                     NKSTWYNSMIMFSFVGLTSAFISLWGVRYIMDVYGVSKSFSAFIVSFFTYGFIFGSII
                     MDFVFAKIRSSKFNIIKFGAMIDLFIWIVIVVVYQVKPPIIVLPISFFIMGCIVMSHL
                     QVFNDAKYKNKEIYSGLATSVINTFEFIGSGIINLIIAISLQVNSYNIVDGYKKG"
BASE COUNT         1792 a          434 c          816 g         1630 t
ORIGIN      
        1 actatagata aatttgtaat tgatgctaac aaaatactat gtaaaaatac tcaagtaaag
       61 tatgctttat tatatataga tattgataaa tttaaatata taaatgattt atttggatat
      121 gaagttggga atgaaatttt gcgtaattta acaaagatta ttaagaggaa tatatttgaa
      181 gaggaaatgt ttgcaagaat atctgcggat aattttatta taattatgaa atatatagaa
      241 gaagaagata taacaaagag gttaaaaact atatttgaag aattagactt gtttaataat
      301 aatcaagaag aaaaatataa gttagtttta agttgtggaa tctattttat acttccagaa
      361 gatagagata tcaactcaat catagatagg gctaatatac ctcataaaat ggcaaaggga
      421 ggacataaaa gttcatatgc attttatgat aataagattc atgaccaaga gataaaagaa
      481 aaagagatgg aaaatacaat gttttcaagt ctagaaaata aagaatttat tatatactta
      541 caacctaaga tagaacttaa tacaggagag atacaaggtt cagaagcact tgttaggtgg
      601 aaacgtcctg ataaagggct aatacctcca aatgaattta tcccattttt tgaaagaaac
      661 ggttttgtta taaatttaga tttatatgtg cttgaagaag tatgtatata ccttagaaaa
      721 tggattgatg caggaataaa tccagttact gtttcagtta atgtttcaag gatacattta
      781 tattgcaata attttataga aacatacaaa aatataattg ataaatacaa tatacctgca
      841 aagtatatag aacttgaatt aacggagagt atcatatttg ataattttga tattttaatt
      901 gatataatga ataatttaaa aaaaatagga tttttaatat ctatggatga ttttggctca
      961 gggtactcat cattaaatat gttaaaagaa attcctatgg atatcttaaa acttgaccaa
     1021 aaatttatta tggaaactta taatagtaaa agaagcaaaa taattgttac taaagtaata
     1081 gaaatggcaa aagagttggg tatgaaagtc atatcagaag gagttgagac agaagaacag
     1141 tttaaacttt taaaagaagt aaagtgtgat atggcacaag gatatttgtt tggaaagcca
     1201 atgccaattg aagaatttga acatttaatt gtatctaact tggtaagagg gtaattaatc
     1261 tgtaatataa agaatttaat aaattgtgtt aaaaatggac tctaatgtag tgttcatttt
     1321 ttttgtaaaa ttttttatac ttttactaaa aagtaattga catatacttt gtattattat
     1381 attattgtat taacaactta atacaataat ataataagga ggcatgaaca tggaatggga
     1441 acttgataat aacaaaccta tatatataca attagtagaa catttaaagt taaaaataat
     1501 ttcaggggaa attaaaatag gatctaaatt agaaacagtg agagcactag cagaagatgc
     1561 ggaagttaat ccaaatacaa tgcaaaaggc tctaacagaa cttgaaagac aaggtttagt
     1621 ttatagtcag agaactaaag gaagatttgt aacagatgat aaggaaaaaa taaaagctat
     1681 gaaagaggaa atagctaatg tggagataaa tacattaaaa gtaaccttag aaaagcttgg
     1741 atatgataga gatgaaatgc taaaacttat tacagaaaat ctgaaaggag aattataaaa
     1801 tggataaaaa tataagcaat gtttctcaag atggaatagt tgaatttaaa ggtgtaaaca
     1861 agagttatgg aactaagaat gttttaaaaa atattgattt aaatatacca aagggaaaaa
     1921 tagtcgggct gttaggacct aatggaagtg gcaaaagtac aatgattaag ctaatgaatg
     1981 gattattaca gcctgataat ggtgaaatta tgataaatgg aatgaaacca tccatagata
     2041 ctaaaaaaat agtttcatat ttaccagaaa gaacttattt aaatgattgg atgaaggtat
     2101 cagatttact taagtttttc tatgattttt atagtgattt tgatgttaga agagcaaatg
     2161 agatgattaa aagcttagat attgatgtaa atgaaaagct aaagacaatg tctaaaggaa
     2221 ctaaagaaaa agttcaactt attttagtaa tgtcaagaaa tgcgagcata tatatattgg
     2281 atgaacctat aggtggtgtt gacccagctg caaggtcata tatacttaag actatattaa
     2341 aaaattattc tgaagatagt actttactga ttgctaccca tttaataagt gaaatagaaa
     2401 acatatgtga tgagataata ttcatatcaa aaggagaaat agtgttacaa ggagacgttg
     2461 aagcaataag agaagaaaaa ggaaaatcta tagatgcatt atttagggag gaatttaaat
     2521 gttaggtaaa ttattaaaat atgagttaaa agcaagtgga agaatattta taccgttata
     2581 tatagccata ttgatagtag ctgtatttaa cggaatattt atgaatacaa atatacttca
     2641 ggtacaggga ataggaatac tagtattaac aagtcttttt atggcattag gagttttaac
     2701 tattgtagtt acaattcaaa ggtttagaaa aaatcttttg ggggatgaag gttatttaat
     2761 gtttactctt cctgttagca ctagttcact tatattatct aaatgtataa cagcacttat
     2821 atacgctgta cttagtttta tagttgcagt attcactttt ggagttctta tgctttttgg
     2881 tacttctgga attttactac ctgaaatttt agatttgttt aacactagtt ttaaatggat
     2941 ttctgaaaac tttttagata ttttattatt agtagtagta atgtttattt catattcatc
     3001 atttattttg ttattatata catcaatctc aatgggacaa ttaccaaaat tcaataaaca
     3061 tagaaacata gtggcatttg cttcttttat agctataaat atagtaatat caattgttgg
     3121 agatgcagtt ggaagcatct taccaaatga agatacaaat atggtatatc atctttatca
     3181 gagtcctagt tttatgcttg caatacttgg tagtcttgta gttgcaatag cgttattttt
     3241 cgctacaaaa tttatattag ataaaaaatt aaatttagaa taaatgtttt tatatggagc
     3301 catcttttga gtaatgttaa aagatggctc ttattattta tgtagttaat tcatgtatat
     3361 aatatgaata gtgttttatt tctagttaaa tatatacata tgttgtataa tgatattgcg
     3421 tgtaaaaatt tgtaagaata actatacact ttatataagt tatatcaaga aaatttaaaa
     3481 atatatggat ttgagggaga agatatgaaa agttatacaa aggctaagtg gagtgtttgg
     3541 ggtataatta cattttcatt tgttttagta ttatttttaa ggatgtcaac tgctgttgtt
     3601 tcagacaatt tagcaaatga gttaggtttt aattctatac aaatttcaaa tatagcatcc
     3661 ttttgcctat atgcatatgc atttatgcaa atacctgcag gaattttgat tgataaatat
     3721 ggagcaagaa aaattagcag tcttggaata ataatggcaa gtttagggtc aattttattt
     3781 ggtttgattc aaagtataga attagcatat atttctcgtg taattgtagg ggcaggaaca
     3841 tcagtaatat tactttgtat tttaaaaatt caagggagat ggtttaataa gtcagaattt
     3901 gcttctgcaa cagctaaatt ttcatttgta ggaaatttag gtggagttct tgctactttt
     3961 ccacttgtgt ttttgtctga acttgttggt tggagaaatt catttttatt aatcggtata
     4021 attggtgttg taattggatg ttttatgtat attattgtta gagatacacc aaaagagtat
     4081 ggattcaata tagatactga accctatgaa aagtcagaga aggttaatat tgttgatggt
     4141 attaagtctg ttataaagaa caaatcaaca tggtataatt caatgataat gttctctttt
     4201 gtgggtctta caagtgcatt tataagcttg tggggagtaa gatacataat ggacgtttat
     4261 ggtgtgagta agagtttttc tgcgtttata gtatcatttt ttacatatgg atttatattt
     4321 ggttcaataa ttatggattt tgtatttgct aaaataaggt ctagcaaatt taatataata
     4381 aaatttgggg caatgataga tttgtttata tggattgtaa ttgtagttgt ataccaggta
     4441 aaaccaccaa ttatagtttt accaatatca ttctttatta tgggatgtat agttatgtca
     4501 catctacagg tttttaatga tgccaagtat aaaaataaag aaatttattc tggactggca
     4561 actagtgtta ttaatacttt tgaatttata ggaagtggaa ttattaattt gattatagct
     4621 atctctttac aagtaaattc atataatata gttgatggat ataaaaaagg tt
//
LOCUS       DAEPIN010000149         4615 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.152, whole genome shotgun sequence.
ACCESSION   DAEPIN010000149 DAEPIN010000000
VERSION     DAEPIN010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4615
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.152"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     misc_binding    308..545
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            609..2528
                     /gene="thrS"
                     /locus_tag="KR250_003696"
     CDS             609..2528
                     /gene="thrS"
                     /locus_tag="KR250_003696"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HBH0069575.1"
                     /translation="MIKVALKDGSIKEFENAISVMDVAKSISEGLARNVVAASVNGEV
                     VGLDHIIDTDCDLNLFKFEDKEGKEVFRHTSAHILAQAIKRLYPEAKLAIGPSIENGF
                     YYDIDLDHRLVPEDLEKIEAEMKKIAKEDLKIERFELPRNEALELMKEQGEDYKVELI
                     SDLPESEIISFYKQGDFTDLCRGPHLPSTKKVKAVKLQSVAGAYWRGDENNKMLQRIY
                     GTSFEKNKDLEEYLHLLEEAKKRDHRKLGKELGLFMIPEEGPGFPMFLPKGMELKNEL
                     LKFWREIHRKAGYIEIESPIILNRKLWETSGHWYHYKENMYTVKIDDEDYAIKPMNCP
                     GGLIYYNSQLHSYRDFPMRVAELGRVHRHELSGALQGLMRVRAFTQDDSHIFMLPEQI
                     KDEIKGVANLIDGIYKTFGFEYNLELSTRPENSMGSDEEWEAAENGLREALEELGLPY
                     TINEGDGAFYGPKIDFHLKDCLGRTWQCGTIQLDMQLPRQFDNTYIGQDGEKHRPVMI
                     HRVAFGSIERFIGILIEHYAGKFPVWLSPTQVKILPISDKFMDYANEVKKELFDKGIR
                     VELDDRAEKIGFKIREAQLEKVPYMLIVGEKEVADNNVSVRSRDKGEIGSIKLDEFIA
                     SISKEIESRESIIQD"
     gene            3296..4387
                     /locus_tag="KR250_003697"
     CDS             3296..4387
                     /locus_tag="KR250_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HBH0069576.1"
                     /translation="MIEKVEEKSKIIKTSKEYKRTIIEEKDKEWTKKLNDILSRSSFS
                     NDELDKIKALIPKEILTNENVGEVVTEDGYAKPEYDEVFKSLFTLNNEYDLLVNFIND
                     ILKDAPYANRNIKPFTQIKRIIRVETDPTINYIGEKRPKLDILAEDEENNHINIEMQR
                     AFENDYLERAEYYLSRVHGRKLEEGKEYKEIGKTIGIHILNHVKYNHIEDYVNCLRLT
                     MDGHPDIYSSKTALYFIELPKIHKSDYIVNRIMLWGKLISNPSNLDVRVIAKNDSIIK
                     KALDRLKELGSNEEYLLNLKRGAYIMNKSRNFKEEIERETAIRVAKEKDYKIIIMLKK
                     NGFKLDDILNKFNDFDLSEDEIKEVYENN"
BASE COUNT         1825 a          460 c          833 g         1497 t
ORIGIN      
        1 ctatattaag tattgattgt atttaagtat acaaaaccta aaaagagtac aaaaattaga
       61 agttagtttt aataaaaatt agttttagta tttatctagt catatctatc atgcatgtgg
      121 atacgtttta aaaattttaa atttatgaaa atattataat atcactataa atcaagagat
      181 taatttaata taaaaatata agaatattgg tttacagctg attgagatga aaaataatgg
      241 gtaattaata tattacatta atgttgattg atgtataact atctgttata atgaaaatat
      301 attaaaaatc atagtgttga agaggaagag tagagtagaa accattctca gagaatttgg
      361 gtctatggct gagagcccat gtaataggtc tatttgaaaa ctaccttgga gctgagctta
      421 ttagcagtat ttgttttgta ctatacgtga aagtgctcgg gtgacgacct tatagtcatc
      481 aagagggttg tatatacttg tatacgccaa cttgggtgga accgcgaatt aatatcgtcc
      541 caaacatatt tggggcgatt ttttattaca aaaaatctaa aaattatttc aataaggagg
      601 aaaaaacaat gattaaagtt gctttaaaag atggctcaat aaaagaattt gaaaatgcta
      661 tttctgttat ggatgtagct aaatccatta gtgaaggatt agctagaaat gtggtagcag
      721 cgtctgtgaa cggagaggtt gtaggtctgg accacattat agatactgat tgtgacttga
      781 atttatttaa gtttgaagat aaagaaggta aggaagtttt tagacataca tcagcacata
      841 tattagctca agctataaaa agactttacc cagaagcaaa gcttgctata ggaccaagta
      901 tagaaaatgg attctactat gatatagatt tagaccatag attagttcca gaggacctag
      961 agaaaataga agctgaaatg aaaaaaatag ctaaagaaga tttaaaaata gaaagatttg
     1021 aacttcctag aaatgaagct ttagagctta tgaaagaaca aggagaagac tataaggtag
     1081 aacttatatc tgatttacct gaaagtgaaa taatatcttt ctacaagcaa ggtgatttta
     1141 ctgatttatg tagaggacca catttaccat ctactaaaaa ggtaaaagct gttaaattac
     1201 aaagtgtagc aggtgcatat tggcgtggtg acgaaaataa taagatgctt caaagaatat
     1261 atggaacttc ttttgagaaa aacaaagact tagaagagta cttacattta ctagaagaag
     1321 ctaaaaagag agaccacaga aaattaggta aagaattagg attatttatg atacctgaag
     1381 aaggtccagg attccctatg tttttaccaa aaggtatgga acttaaaaat gaattattaa
     1441 aattctggag agaaatacac agaaaagctg gatatattga gatagaatct ccaatcatat
     1501 taaatagaaa attatgggag acttcaggac actggtatca ttacaaagaa aatatgtata
     1561 cagttaaaat agatgatgag gattatgcta taaaacctat gaactgtcct ggtggattaa
     1621 tttattataa ctctcaacta cattcatata gagatttccc aatgagagtt gcagagcttg
     1681 gtagagttca tagacatgag ttatctggtg cattacaagg attaatgaga gttagagcat
     1741 ttactcaaga cgattcacat atattcatgt taccagagca aattaaggac gaaataaaag
     1801 gagtagctaa cttaatagat ggtatataca aaacttttgg atttgaatat aatttagaat
     1861 tatctacaag accagagaac tctatgggaa gcgacgaaga gtgggaagca gcagaaaatg
     1921 gattaagaga agctttagaa gagttaggtc taccatatac tattaatgag ggtgatggag
     1981 cattctatgg tcctaagata gacttccatc taaaagattg tttaggaaga acttggcaat
     2041 gtggtactat ccaattagat atgcagcttc caagacaatt tgataatact tatattggtc
     2101 aagatggtga aaagcataga ccagttatga ttcatagggt tgcttttggt agtatagaaa
     2161 gatttatagg tatattaata gagcattatg ctggtaaatt cccagtttgg ttatctccaa
     2221 ctcaagttaa gatacttcct atatctgata aatttatgga ttatgcaaat gaagttaaga
     2281 aagagttatt tgataagggt ataagagttg aattagatga tagagctgaa aaaattggat
     2341 ttaagataag agaagctcag cttgaaaaag tgccatacat gctaatagta ggtgaaaaag
     2401 aagtagctga taataatgtt tctgttagat caagagataa aggtgaaata ggtagtatta
     2461 aattagatga atttatagct agtatttcaa aagaaattga atcaagagaa agtattattc
     2521 aagactaata tatattatat tagtagctaa gatattgtta tataagataa gtttaatcaa
     2581 tatgttgata tgttgatgca tagtgattaa atttatgaaa agatgagtat aattagaggt
     2641 acagttaatc atatttttat gattaatgta cctctagttt tttgtatggt agatttatta
     2701 ataaatatat catagaagtg ttttgaacat ggaaatattt atgtgcattc tttaaataaa
     2761 cgtttatttt ttgagtgata attaagtgct tgttggtaag gttatggctg taaaaatcat
     2821 aactttattt tcatatttgt tccattttca taaaaattat tatcccttta agaaaatttc
     2881 ttaatcctct tgaaatattt cctttttagt ctaaagttaa atttctttct tcaaaatgaa
     2941 tatttaaatg aactaaaatt taagtacatt ttctaatctt atgagtctat ttttcataga
     3001 taattgccct ataatagaag aatctatgtt ggtgctatgt ggtgacaata acagcattgt
     3061 cagtgattaa ttaatgcttt ataaatgatt aattagtgtt ttatgttatt tttggactta
     3121 ccctctatac atagattaat ttatttaact ttagatagag agagaatgtc aagtacatac
     3181 acagacatta gagtttaaga aagtgtttat ggagcaagaa cttttaaaag aggtagtgac
     3241 ttagtttaat ttcaagaaat gataaataca aaagaggaaa aagagaggtg ttgaaatgat
     3301 agaaaaagta gaagaaaaat ctaagattat taaaacatca aaagaatata agcgaactat
     3361 aattgaagaa aaagataaag aatggaccaa aaaattaaat gatatactta gtagaagtag
     3421 tttttctaat gatgaattag ataaaatcaa ggcgttaatt ccaaaagaga ttttgactaa
     3481 tgaaaatgtt ggagaagttg tgactgaaga tggatatgct aagcccgagt atgatgaggt
     3541 atttaaatca ttgttcactt taaataatga atatgattta cttgtaaatt ttataaatga
     3601 tattctaaaa gatgctccat atgctaacag gaatatcaaa ccgttcactc aaataaagag
     3661 aataattaga gttgaaactg atccaacaat taattacatt ggagagaaaa gacctaaact
     3721 tgatattttg gctgaagatg aagaaaataa tcatataaat attgagatgc aaagagcttt
     3781 tgagaacgat tatttggaaa gagctgaata ttatcttagt cgtgtacatg gtagaaagtt
     3841 agaagaaggt aaagagtata aagaaattgg aaaaactatt ggaatacata tactaaatca
     3901 tgtaaaatat aatcacatag aagattatgt aaattgcctg agattaacaa tggacgggca
     3961 tccagatatt tattctagta aaactgcatt atattttata gagcttccaa aaattcataa
     4021 atctgactat atagtaaata gaattatgct atggggaaaa cttattagta atccttctaa
     4081 cttagatgta agagttatag ctaagaatga ttctattatt aaaaaagcac ttgatagatt
     4141 aaaagaatta ggtagcaatg aagaatattt attaaatctg aaaagaggtg cttatatcat
     4201 gaataaaagt agaaatttta aagaagagat tgaaagagag acagcaatta gagttgcaaa
     4261 agaaaaagat tataaaataa ttataatgtt gaaaaaaaat ggttttaaat tagatgacat
     4321 attaaataaa tttaatgatt ttgacttaag tgaagatgag ataaaagaag tatatgaaaa
     4381 taattgatga taaataaaaa gtgaacttat ataagtttgc tgaatcggag tctggaaata
     4441 tttatgtgta ttttttaatg aatgaaaatt tattttaaaa acaataattt agaagtacag
     4501 ttaattatat ttttatgata ctgtacttct agttttttct attgtagatt tattatttac
     4561 taataaatct acaatagaaa tgcttttgtt gtaagaatca ttttatctaa tgatt
//
LOCUS       DAEPIN010000150         4343 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.153, whole genome shotgun sequence.
ACCESSION   DAEPIN010000150 DAEPIN010000000
VERSION     DAEPIN010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4343
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.153"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            188..526
                     /locus_tag="KR250_003698"
     CDS             188..526
                     /locus_tag="KR250_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3784 domain-containing protein"
                     /protein_id="HBH0069577.1"
                     /translation="MFAVGFVVIIALAFFILSVLLFNGKASMLLAGYNSMSKKEKARY
                     DKKKLCRSSSIVTFIVSIMLFIMAFLGYRIESGQMDEKKMVPFSIIFIIVILSSVFFN
                     ILYSNKKCKK"
     gene            complement(933..2369)
                     /gene="miaB"
                     /locus_tag="KR250_003699"
     CDS             complement(933..2369)
                     /gene="miaB"
                     /locus_tag="KR250_003699"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HBH0069578.1"
                     /translation="MSKRKQVTIPIEKIQEQDKYIEQIHRQNEEYFNRTGKRKLVFTQ
                     TFGCQMNEHDSEKLCSMLEEMGYQMSMMVEESDLIIYNTCAVRENAELKVYGNLGQLK
                     HLKGKNPDMKIAVCGCMMQQPHVVEELRKKYKHVDLIFGTHNLYKFPQLLTESINSDK
                     MLVDVWDVDGEVIEGLRSNRKFELKAFVNIMYGCNNFCTYCIVPYTRGRERSRTPEDI
                     INEIKELVANGTKEITLLGQNVDSYGKTLENPVSFSELLRKVNDIEGIERVRFMTSHP
                     KDISDEVIYAIRDCDKVCEFLHLPIQCGSSSLLKKMNRHYTKEYYLEIIEKAKKEVPG
                     IAFSTDLMIGFPGETEEDLLDTLDVVEKVRYDSAFTFIYSKRQGTPAAKMENQIPEDI
                     KHDRFNRVLEAVNRISAEINDGYKDRIVEVLVEGRSKNNENKFAGRTRQNKLVNFEGG
                     NDDLIGKLVMVKITEPRTFSLNGILVNN"
     gene            complement(2617..4077)
                     /locus_tag="KR250_003700"
     CDS             complement(2617..4077)
                     /locus_tag="KR250_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp28"
                     /protein_id="HBH0069579.1"
                     /translation="MKFYKRILTLTLSISFVFANIQLVDAISSVEKIQGKNKYEIAGK
                     IADKNAYKTAYKTAYKTAILINTSNSIADGLSASGLAGALNAPILLTEKNTIPTETSA
                     RLKNVSKVYIIGGTYSISTSVENSLKSKKMKVVRIKGNDRIKTSYNVAKEINSIKKVN
                     TVMLTNAYKGEADAISIASVAARDKAPIILTNGQSIPFSTSGLKSYVIGGTASMSTTL
                     VNNTKSTRLGGSTRFETNKAIINKFYKDAREFYIAGAYELTNALVGSSLSKHGPMVLV
                     NDGSNKSILKNAKKITSIGYIDSNIVQQCLNITNGIGDINTGVVKNVKPTTKTIKDGM
                     YKVGKDISAGEYLITSNSGSYASYYEVTSDSTGNADSILSNDIFSGTRYITLKNGQYI
                     KIEDSTMTLAKYAKAQKAKNGKFGNGMYKIGLEIPAGEYIIMSNSSDAYYEVRNDSLG
                     NAEGIVTNDTFSGRRYITVEEDQYLILNDCYLIENE"
BASE COUNT         1408 a          671 c          461 g         1803 t
ORIGIN      
        1 attgtttgaa tatttgatgg ttttcaatgc aatttcaaca taaatactat gtattagtgt
       61 atactgttga tgaattaaag tatgataatc tatttaataa agtatgaagt aaatgatttt
      121 aatgtgcctt tatggtaaaa ttactaataa gtaataaaat taattaaaat atatggaggt
      181 gcttataatg tttgctgtag gatttgttgt tataattgca cttgcctttt tcattttaag
      241 tgtactatta tttaatggaa aagccagtat gctactagca ggttataact ctatgagtaa
      301 gaaagaaaaa gcacgatacg ataaaaaaaa attatgtaga agttctagca ttgttacttt
      361 tattgtctct ataatgctat ttattatggc gtttttggga tatagaatag agagtggtca
      421 aatggacgaa aaaaaaatgg ttcccttttc tataatattt ataatagtca tactttccag
      481 tgtatttttt aatatcctct actctaacaa aaaatgtaaa aaataaatag ttgtaagttg
      541 agcagttaat tataaaagat tgactgcttt ttcatgtata ttgactactc ttattaaata
      601 ttttaacatt taaaaatcta tgtaaaaata atgaatatag tacaaacttc taataatgat
      661 tttacctttg tggttacagt ccactatata taatttaata tgataacagg gaattactaa
      721 tgacaactag gtaagctttt ataaaatata ataaaacaaa aatagatgta caatgaaatt
      781 atatcttata tcaaatttat ttttattaat agcaaaaact tacattagca taacatatga
      841 caaattcgct taaaataaaa atttttaata taatttgttc tatagtacat ctattttgat
      901 tagtttacat aattttatta ttttgtacaa tattaattat ttactaatat cccatttaat
      961 gaaaaagttc taggttcagt aatttttacc ataactaatt taccaattaa atcatcattt
     1021 ccaccttcga aatttacgag tttattttgt ctagttctac cagcaaactt attttcatta
     1081 tttttacttc ttccttcaac tagaacttca actattctat ctttatatcc atcatttatt
     1141 tctgcagaaa tcctatttac agcttctaac accctattaa atctatcatg ctttatatct
     1201 tctggtattt gattttccat tttagcagca ggagttccct gtcttttaga ataaataaat
     1261 gtaaaagctg aatcatatct aactttttca accacatcta aagtgtccaa taaatcttct
     1321 tctgtttctc ctgggaaacc aatcattaag tctgtagaaa aagcaatacc tggaacttcc
     1381 tttttagctt tttctattat ttctaaataa tattcctttg tataatgtct attcattttt
     1441 ttcaaaaggc tactgcttcc acattgtatt ggtagatgta aaaactcaca caccttatca
     1501 caatctctaa ttgcgtatat tacttcatca gatatatctt ttggatgaga tgtcataaat
     1561 cgaacccttt caattccttc tatatcattc acttttctta aaagttctga gaatgaaact
     1621 ggattttcaa gtgtcttacc ataagaatct acattttgtc caagtaaagt gatttctttc
     1681 gttccatttg caacaagctc ttttatctcg ttaattatat cttcaggagt tctacttctt
     1741 tctcttcctc ttgtgtatgg gactatacaa tatgtgcaga aattattgca tccatacatt
     1801 atatttacaa atgcttttaa ttcaaatttt ctattacttc taagaccttc tattacttct
     1861 ccatccacat cccatacatc cactaacatt ttgtcagaat ttattgattc tgtaagtaat
     1921 tgcggaaatt tatatagatt atgtgttcca aatataaggt ctacatgttt atattttttt
     1981 cttaattcct ctacaacatg tggttgttgc atcatacatc cacatacagc tattttcata
     2041 tctggatttt ttccttttag atgttttaat tgtcctaagt taccatacac ttttagctct
     2101 gcattttctc taactgcaca ggtgttatat attattaaat cactttcttc aaccatcatt
     2161 gacatttggt atcccatctc ttctaacatt gaacataatt tttctgaatc atgttcattc
     2221 atttgacacc cgaaggtttg agtaaacacg agttttctct ttccagttct gttgaagtat
     2281 tcttcatttt gtctgtgtat ttgttctata tatttatctt gttcttgtat tttctctata
     2341 ggtattgtta cttgttttct tttgctcacg tctattcctc ctagttaatt tcttatatta
     2401 cacataacct taattatatc aaataagaaa aatcttttaa agatattatt atttttctag
     2461 ttgtgtttta atagtaaatc taatgattta tatatactat atttctaaaa tataaaatca
     2521 tataattatt ataataataa aaagatgttt taaattaaaa cttaaaacac ctttccttaa
     2581 ataaaatata tgatttacta ttaaatacaa ttacatctat tcattttcaa ttaaataaca
     2641 atcatttaat attaaatatt gatcttcctc aaccgttata tatcttcttc ctgaaaaagt
     2701 atcattagta actattccct ctgcattacc taagctatca tttctaactt cataatatgc
     2761 atcagaagaa tttgacatta taatatattc tccagcagga atttctaatc caattttata
     2821 cataccattt ccaaacttac cattcttggc cttctgggct ttagcatact ttgctaatgt
     2881 cattgttgaa tcctcaattt taatatattg accattcttt aaggtgatat atctagtccc
     2941 actaaaaata tcatttgaaa gtatactatc agcattacct gtactatcac ttgtaacttc
     3001 ataataagaa gcataacttc ctgagtttga agttattaag tattcacctg ctgagatatc
     3061 ttttccaacc ttatacattc catcttttat agtctttgta gtaggtttaa cattttttac
     3121 aaccccagta ttaatatctc caattccatt tgttatattt aaacactgtt gtactatatt
     3181 tgaatctata tatcctatag aagttatctt cttagcattt tttaaaatgc ttttattgct
     3241 accatcgtta actaaaacca ttggaccatg ttttgataaa gaagatccta caagtgcatt
     3301 tgttaattca taagctccag ctatataaaa ctctctagca tccttataaa acttatttat
     3361 tattgcttta ttagtttcaa aacgagtact cccccctagt ctagtagatt tagtattgtt
     3421 tactaaagtg gtactcattg aagctgttcc tcctattaca taagacttta aaccactagt
     3481 tgaaaaaggt atactttgtc cattagtaag aattattggt gctttatctc ttgctgctac
     3541 agatgcaata cttatagcat ctgcttctcc tttatatgca ttggttagca taacagtatt
     3601 gacttttttt attgaattta tttcttttgc aacattataa cttgttttta ttctgtcatt
     3661 tcccttaatt cttacaactt tcattttctt agattttaat gaattttcga cagatgtact
     3721 aatagaatat gttcctccaa ttatataaac ttttgataca ttttttaatc ttgcagatgt
     3781 ctcagttggt atagtatttt tttcagttaa tagtataggg gcatttaaag ctcctgctag
     3841 tccacttgca cttaatccat ctgcaataga gttactagta ttaattaata tagcagtttt
     3901 atatgcagtt ttatatgcag ttttatatgc atttttatct gctattttac ctgctatttc
     3961 atacttattt tttccttgta ttttttctac agatgatatt gcatctacta attgaatatt
     4021 tgcaaataca aaagaaatac ttaaagtcaa tgttaatatt cttttataga atttcataat
     4081 aaaattgtcc ccctcataat aaatttttaa tatataaata catattctta aattagttat
     4141 gtacttaaca aacttatgta aaattgcaaa ttatattaag tatattacaa ataaatatta
     4201 atacaattat tcatttagct ataattgtat ttacatttat attaaaaatc aactagattt
     4261 gtataaatac ttataattat atttttattc ttaatatttt ttttattatg ttattttaat
     4321 agatttattt ttctttttta agc
//
LOCUS       DAEPIN010000151         4238 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.154, whole genome shotgun sequence.
ACCESSION   DAEPIN010000151 DAEPIN010000000
VERSION     DAEPIN010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4238
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.154"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            24..323
                     /locus_tag="KR250_003701"
     CDS             24..323
                     /locus_tag="KR250_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069580.1"
                     /translation="MKWIKWYSITCICIFIVVAFYMFIFPNKIETIDTSSAYSFVEKK
                     VPNSAVYQGYKKNPVDGTTTIYYSYDNSTHIVRLSHPEDYSRKINWDKVSNIRFD"
     gene            647..2302
                     /gene="ilvD"
                     /locus_tag="KR250_003702"
     CDS             647..2302
                     /gene="ilvD"
                     /locus_tag="KR250_003702"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HBH0069581.1"
                     /translation="MRSDIKKGIEGAPKRALMYGMGLTKEEIERPLIGIVNAQNEVIP
                     GHLHLDEIAEAAKNGVRMSGGLPLEFPAIGVCDGIAMGHVGMNYSLASRELIADSIEA
                     MAMAHGFDALVLIPNCDKIVPGMLMAAARLNIPSIVVSGGPMLPGKKNGKVYDFNSAM
                     EGVGACKDGTVSEEELEALAMNSCPGCGSCSGLFTANSMNCLTEALGMGIPYNGTAAS
                     HSGERKRIAKYAGMYVMELLKNDIKPRDILTIDAFKNAIAVDMAMAGSTNTVLHLPAI
                     AYESGIELNLNFFDEISEKTPCLTKLSPSGKHHIEDLHMAGGIPAIMNELSKINGINL
                     DCKTVTGKTIGENIRNCEIENEEVIHTLKNPYSNQGGLAILKGNLALNGAVVKKSAVA
                     EEMLVHEGPARVFNSEEEAVNAIFGKKINKGDVIVIRYEGPKGGPGMKEMLSPTSAVA
                     GMGLDKHVALLTDGRFSGATRGASIGHISPEAMEGGLIGLVEEGDIISINIPDKKLEL
                     KVDEVEIENRKLKFKPLEPKIKHGYLSRYAKLVTSANTGAVLK"
     gene            2646..3278
                     /locus_tag="KR250_003703"
     CDS             2646..3278
                     /locus_tag="KR250_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBH0069582.1"
                     /translation="MNREEKSKNSKEKIIQSAFSLFSSKGYDSTSTQDIINLSGLSRG
                     AMYHHFKTKEDILRSVTKELYSQMNNFLEHLVADDTLTANEKIIELVVHSANDYTRRK
                     MVHCSWLEKIPFALIEEVRNLNNVVAPNIAKIIKQGVENKEFSCEYPEELAEMLVFSI
                     DILLDPVLFKREYSEVCNRLDFLLFMLKKMDIPLIDEYGIQKFKDLFRQL"
     gene            3301..4113
                     /locus_tag="KR250_003704"
     CDS             3301..4113
                     /locus_tag="KR250_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBH0069583.1"
                     /translation="MFYNAKSCQMMIGSNTVNYIEFGSGKKTLIILPGLGDGLFPLHG
                     KIQAIAFAFRYKQFAKDYKVYVFSRKNQITEKYSTRDMAKDQADIMKKLGIMKAEVMG
                     VSQGGMIAQYLAIDYPELVEKLVLAVTSSKQNDTIQNVICSWIDMAKKQNYNDLMIDT
                     AKKSYSERYLKKYQLFIPFLGKVGKPKDFKRFIIQATSCIEHNAFSELNKITCPTLII
                     GGANDKIVGNNASFHLAEKIKKSEIFIYEGLGHATYEEAQDFNERVLEFLNK"
BASE COUNT         1636 a          482 c          783 g         1337 t
ORIGIN      
        1 tttggtaaag taggaggaat tttatgaaat ggattaaatg gtatagtatt acatgcatat
       61 gtatttttat agtagtagct ttttatatgt ttatatttcc taataaaata gaaactattg
      121 atacttcatc tgcatatagc tttgttgaaa agaaagtacc aaatagtgct gtgtatcaag
      181 gatacaaaaa gaatccagtt gatggtacta ctacaattta ttacagctat gataattcaa
      241 ctcatatagt tagattatca catccagaag actattcaag gaagattaat tgggataaag
      301 tttcaaacat cagatttgat taatctttaa tatttataaa agtctatata tctaaggcaa
      361 atatattatt aatcttattc atagttgagc actttctatt atagaagtgc ctcttctgat
      421 ttatatcata aaatattact caacaatttg taaaatatat tagtttataa aatatattga
      481 aaagataata ttatataata caattgtgta agatattgat aaaaaatcgt aataaatttt
      541 cattagtata tgctaatatt tttttacaat tttcgtaatt aattaataga aaaatatatt
      601 ataataatat tatgaaaatt taaaaaagta agaaagaggt aatattatga gaagtgatat
      661 aaaaaaagga atagaaggag ctccaaagag agcgttaatg tatggaatgg gtcttactaa
      721 agaagaaatt gaaagaccat taataggtat agtaaatgca caaaatgagg tgattcctgg
      781 tcatctgcat cttgatgaaa tagcagaagc tgcaaaaaat ggagtaagaa tgagtggtgg
      841 tttgccatta gaatttcctg ctataggggt atgtgatggt atagctatgg ggcatgttgg
      901 aatgaattat tccctggctt caagagaatt aatagcagat tcaatagaag ctatggctat
      961 ggcacatggt tttgatgctt tggtacttat acctaattgt gacaagatag tgccaggtat
     1021 gttaatggct gctgcaagac ttaatatacc aagtattgtt gtgagtggag ggcctatgct
     1081 tccaggtaaa aaaaatggaa aagtatatga ttttaactct gcaatggagg gtgttggagc
     1141 atgtaaagat ggaacagtaa gtgaagaaga attagaagcg ttagccatga actcttgtcc
     1201 tggatgtggt tcatgttctg gtcttttcac agcaaatagc atgaattgtt taacagaagc
     1261 tttaggcatg ggtataccat ataatggtac tgctgcgtca cattctggag aaagaaaaag
     1321 gatagcaaaa tatgcaggta tgtatgttat ggagttactt aagaacgaca taaaacctag
     1381 agatatttta acaatagatg cttttaaaaa tgctatagct gtggatatgg caatggctgg
     1441 ttctacaaat acagtacttc acttacctgc aatagcttat gaatcaggaa tagagcttaa
     1501 cttaaatttt tttgatgaaa taagtgaaaa aactccttgt ttaacaaaat taagtccaag
     1561 tggaaaacat catattgaag atttacatat ggcaggagga ataccagcta taatgaacga
     1621 actttcaaag ataaatggaa taaatttaga ttgcaaaacc gtaacaggca agactatagg
     1681 ggaaaatata agaaattgtg aaatagaaaa tgaagaagta atacatacat taaagaatcc
     1741 atatagtaac caaggtgggc ttgcaatatt gaaaggaaat cttgctctaa atggagctgt
     1801 tgtaaaaaag tcagcagttg cagaagaaat gttagttcat gaaggaccag caagagtttt
     1861 taattcagaa gaagaagctg taaatgctat ttttggtaaa aaaataaaca aaggtgatgt
     1921 tatagttata agatatgaag gtccaaaggg tggtccagga atgaaagaaa tgctatctcc
     1981 tacatcagca gttgcaggaa tgggacttga taagcatgta gcacttctta ctgatggtcg
     2041 tttttcagga gcaactagag gagcatctat aggccatatt tctccagaag ctatggaagg
     2101 tggtttaatc gggttagttg aagaaggaga tataatttct ataaacatac cagacaaaaa
     2161 attagagtta aaagtagatg aagttgaaat agaaaataga aaattaaaat ttaaaccttt
     2221 agaaccgaaa ataaagcatg gatacttaag tagatatgct aaactggtaa catcagcaaa
     2281 tacaggagca gttttaaaat agtagactat atttatgtaa attagaaaaa gttaagaaaa
     2341 tattaattgg atatcttagg tgttatagca ctaatgaggt atccttttta ttttgtatga
     2401 aaaacacagc aaaatcttga ttatctttga aaacaataca tctctattat aaggaatacg
     2461 agtacagcat agagatgtat cttttagacc ttgttgaggt aatacaactt tttaaaagtt
     2521 tgatgtggaa tgcttttcgt tccatctgaa ctatgaagaa attgaaagtc agttaattct
     2581 tgacataata aatattaaaa tgtataataa aaaacaaaca gacagtctgt ttttaggagg
     2641 gaaatatgaa tagagaagaa aaaagtaaaa atagtaaaga aaaaattatc caatcagcat
     2701 tttcactatt ttcgtctaaa ggatatgatt caacatctac acaagatatt atcaatttat
     2761 ctggtctatc tagaggtgca atgtatcatc actttaaaac taaagaagat atactgagaa
     2821 gtgtcacaaa agaactttac tcacaaatga ataatttttt agagcatctt gttgctgatg
     2881 acacccttac agcaaatgaa aaaataatag aattggttgt tcatagtgcg aatgattaca
     2941 cacgtagaaa aatggtacat tgtagctggt tagaaaaaat cccattcgct ttaatagagg
     3001 aagttcgtaa tctcaacaat gtagttgcac ccaatattgc taagataatt aaacaaggtg
     3061 ttgaaaataa agaattttct tgtgaatatc cagaggaatt agctgaaatg ctcgttttta
     3121 gtattgatat tttacttgac cctgtattat ttaaacgtga atacagtgaa gtatgtaata
     3181 ggttggattt tttattattc atgttaaaaa aaatggatat tcctctaatt gatgagtatg
     3241 gtattcaaaa atttaaagat ttatttagac aattataata ggaaaagcga ggaatagact
     3301 atgttttata atgcaaaaag ttgtcaaatg atgataggaa gtaatacagt gaattatatt
     3361 gaatttggta gtggtaagaa gacactaatt attttaccag gattaggaga tggtttattt
     3421 cctttacatg gaaaaataca ggcgattgct tttgctttta gatataagca atttgcaaaa
     3481 gattataagg tttatgtatt tagtagaaaa aatcaaatta cagaaaaata ttccacaagg
     3541 gatatggcta aagaccaagc agatataatg aaaaaacttg gaattatgaa agcagaagta
     3601 atgggtgttt ctcaaggtgg aatgatagct caatatctgg caattgatta tcctgagtta
     3661 gttgagaaac ttgtgttggc agttacttct tcaaagcaaa atgatactat ccaaaatgtt
     3721 atttgtagtt ggattgatat ggcgaagaag cagaattaca atgatttaat gattgacaca
     3781 gcaaagaaat cctattctga aagatattta aaaaaatacc aattatttat tcctttttta
     3841 ggaaaagtag gtaaaccaaa ggattttaaa cgatttatta ttcaagcgac ctcttgcata
     3901 gaacataatg ccttttccga attaaataaa ataacatgtc caaccttaat tataggaggt
     3961 gctaatgata aaattgttgg aaataatgct tcttttcatt tagcagaaaa gattaaaaaa
     4021 agcgaaatat ttatctatga gggattggga cacgctacat atgaagaagc acaggatttt
     4081 aatgaaagag ttttagagtt tttaaataag taaacacctt tgttaaaaat atacatatga
     4141 agattggaaa tttaatgtta aaaatagaaa catgaaaata tgcttaactg gtatttttac
     4201 tattcataac caatttttga tacattatct actataaa
//
LOCUS       DAEPIN010000152         4236 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.155, whole genome shotgun sequence.
ACCESSION   DAEPIN010000152 DAEPIN010000000
VERSION     DAEPIN010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4236
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.155"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            complement(286..4011)
                     /gene="cbpA"
                     /locus_tag="KR250_003705"
     CDS             complement(286..4011)
                     /gene="cbpA"
                     /locus_tag="KR250_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding adhesin CbpA"
                     /protein_id="HBH0069584.1"
                     /translation="MQQKCKKKALSMILSISMGLGSVASFNASNTFADTIEESTNAGN
                     AEGDTNAGNAEGDTNAGNAEGDTNAGNAGGDANAGNAGGDANAGNAGGDANAGNAGGD
                     ANAGNAGGDANAGNAEEDSNAGNAEGDANAGNAEEDANAGNAEEDSNAGNAEEDMNAL
                     VVEEATAEDPTSVGDFKISGGEINVDFKFASGVLTILSGKQMTIQNNNPNTPTTHRIV
                     IPKDVKAELIFDGVNIKTASNSPFTLTPDKDGGAYAHVILQDGSENKLESGDVYYPGM
                     RAGKGTTLTIDDSVVNRDIEGEFIVPEGGKIPRDVTLENGDKLKKGDRLTLLDSNNRG
                     SLKVVGNYYSAGIGGGNTESGGNIIINGGAITAIGQESSRNTGYGAGIGGGGNGSGGD
                     ITINGGTITAKGAYHGAGIGGGYYTTTKNALSPSVVDPSTGSGYSGNITINGGLSYSY
                     GGAHGSAFGDGCVNPGTNRGYRILVTGGTILPYMTGVGDWIFDLSATTGDVIVTGGSL
                     KATTFKSLGGAVAYGDLEKKTKVFMNKISLKYLGQDKVSTTLVDDFKMLIDGKIYPYG
                     APSYTDDQGVLYLWFSDQFKGSEVSVNLVVIDKITGGNIKPDDFYVKDIGSGSQFLKQ
                     YEEFTIDDTPPYLIIKRYDGLPFEEDVEKLFLDWILKDGGIPIITPKGEKLTDKKFMT
                     ISSQRLNEETMEPDTNLGSDDLTKQVDAGKYQLSITSTQFSNVYPFNEAYWGHRAYYK
                     AEIIPADTETSLVVEESKAKTDVFKPTDTFTLNVTISPDKKEGPDCASPRGYVQFYIN
                     GKEYKDPVELKTHNKGSESHNYSTASIEWRPLDSNHHIFTTEQTITVKYIGDDINYIE
                     GSEDEKKLKLDMTNVDVDGDGIPDINIDTDGDGRPDINIDTSGDWKPDINIDTDNTGE
                     WKPSTEGGNGDGIWKPDKNIDTNGDGNPDTDYNRPAIDTDNDGVDDYWKPDKNVDTGS
                     GGYDTGNPNLNKPDPDNGGNSKPDPDNGGNSKPDSGNGGNSKPDSGNGGNSKPDSGNG
                     GNSKPDSGNGGNSKPDPDNGGNSKPDPDNGGNGKPDPDNGGNSKPDPDNGGNSKPDPD
                     NGGNSKPDIDTDGDGKPNINIDIDGDGKPDINIDTDGDGKPDINIDIDGDGKPDINID
                     IDGDGKPDINIDIDGDGKPDINIDTDGDGKPDINIDTDGNGKPDINIDTDESLDSNVG
                     QNVQTGDQSNIMLDLALMFISLFFLIKNLTNKYLRRK"
BASE COUNT         1185 a          921 c          459 g         1671 t
ORIGIN      
        1 ataatattga gtgtttggct tgtatttaca aattattatt ctaacaaaaa caactataca
       61 tagattaaaa tcaccttcta aacataatta ttaaaattta attaatgata tcaattccat
      121 taatctcttt taataaaaaa ttctcttttc tatatgaaaa ttatttcaaa acttttaagt
      181 taaatacaaa aatgactata aatagatttt tatttacaca aatctattta tagtcactat
      241 agtttatcca ataaaataat aaaataaata tgtacacaat aaatgttatt tacgtcttaa
      301 gtatttattt gttagatttt taattaaaaa aaacaagcta atgaacatta atgctaaatc
      361 tagcattata ttactttgat ctcctgtttg tacattttga cctacattac tatctaaact
      421 ttcatctgta tctatattta tatctggttt tccattccca tctgtatcta tatttatatc
      481 cggctttcca tccccatctg tatctatatt tatatccggt tttccatctc catctatatc
      541 tatatttata tctggttttc catctccatc tatatctata tttatatctg gttttccatc
      601 tccatctata tctatattta tatctggttt tccatctcca tctgtatcta tatttatatc
      661 tggttttcca tctccatcta tatctatatt tatattcggt tttccatccc catctgtatc
      721 tatatctggt ttgctatttc ctccattatc tgggtctggt ttactatttc caccgttatc
      781 tggatccggt ttgctatttc ctccattatc tggatctggt ttaccattgc caccgttatc
      841 tggatctggt ttgctatttc ctccgttatc tggatccggt ttgctatttc ctccattacc
      901 tgaatccggt ttgctatttc ctccattacc tgaatctggt ttgctatttc ctccattacc
      961 tgaatccggt ttgctatttc ctccattacc tgaatctggt ttgctatttc ctccattatc
     1021 tggatccggc ttgctatttc ctccattatc tggatctggc ttatttagat ttggattacc
     1081 tgtatcataa cctcctgagc cagtatctac atttttatca ggcttccaat aatcatctac
     1141 tccatcatta tctgtatcta ttgctggacg attataatct gtatcagggt ttccatctcc
     1201 attggtatct atatttttat caggtttcca aataccatct ccatttccac cttctgtact
     1261 aggcttccat tcacctgtat tatcagtatc tatgttaata tctggcttcc aatcaccact
     1321 cgtatctata ttgatatctg gccttccatc accatcagtg tctatattta tatccggtat
     1381 tccatcccca tctacatcta catttgtcat atctaacttt aacttctttt catcctcact
     1441 accttcaatg taatttatat catctccaat atattttaca gtaatagttt gttcagtagt
     1501 aaaaatatga tgattgctat ctaaaggtct ccattcaata gatgcagtac tataattatg
     1561 actctcacta cccttattat gagtttttaa ttctactgga tctttatatt cctttccatt
     1621 aatatagaat tgtacgtaac ctcttggaga ggcacaatct ggtccttctt tcttatctgg
     1681 tgaaatagta acatttaatg taaacgtatc tgttggttta aaaacatcag ttttagcttt
     1741 agactcttct acaactaatg aagtttctgt atcagcagga ataatttctg ccttataata
     1801 tgctctatgc ccccaataag cttcattaaa aggatataca tttgaaaact gtgttgatgt
     1861 aatgcttaat tgatattttc cagcatcaac ttgctttgta aggtcatcac ttcctaaatt
     1921 tgtatctggt tccattgttt cctcatttaa acgttgagaa ctaatagtca taaatttttt
     1981 atctgtgagt ttttcacctt taggagtaat tattggaata cccccatctt ttaatatcca
     2041 atctaaaaat aatttttcta catcttcttc aaatggtaat ccatcatatc ttttaataat
     2101 caaatatggt ggagtatcat caatagtaaa ttcttcatat tgctttaaaa attgagaacc
     2161 tgaacctata tctttaacat aaaaatcatc tggttttata tttcctccag taattttatc
     2221 tataacaact aaattaacag atacttcaga tcccttaaat tgatcagaaa accataagta
     2281 aagaacccct tgatcatctg tataactagg tgctccataa ggataaattt ttccatcaat
     2341 tagcatttta aaatcatcaa ctaatgtagt actaacttta tcttgtccta aatactttaa
     2401 agatatttta ttcataaaaa cttttgtttt cttttctaaa tcaccgtatg caactgctcc
     2461 acctaaactt ttgaatgttg ttgcttttaa tgaaccacct gttactataa catctcctgt
     2521 tgtagcactt aggtcaaaaa tccaatctcc cactcctgtc atataaggta aaatcgttcc
     2581 tccagttaca agtatacgat atcctctatt tgttcctgga ttaacacatc catccccaaa
     2641 tgcactacca tgtgcacctc catatgaata ggaaagtcca ccattaattg taatatttcc
     2701 actataacca gaacctgtgc taggatctac tactgaggga cttaatgcat tctttgttgt
     2761 tgtatagtat cctccaccta ttccagctcc atggtatgca cctttagcag ttatagtacc
     2821 accattgatt gtgatatctc caccactacc attaccgcct ccacctattc cagctccata
     2881 tcctgtatta cgactcgatt cttgacctat agcagttata gcaccaccat taataataat
     2941 atttcctcca ctttcagtat ttccaccgcc aatacctgca gaataataat ttccaacaac
     3001 ttttaaactt cctctattat tactatctag tagtgtaagg cgatctcctt tttttaactt
     3061 gtctccattt tctagagtta catctctagg aatttttcca ccctcaggaa ctataaactc
     3121 tccttcaata tctctattta caacagaatc atcaatagtt agagtagtac cttttcctgc
     3181 tctcatacct ggataatata catcacctga ctctagcttg ttttcactac catcttgtaa
     3241 tataacatgt gcatatgcac caccatcttt atctggtgtt aaagtaaatg ggctattaga
     3301 tgcagtttta atatttactc catcaaatat taattcagct tttacatctt taggtataac
     3361 aatcctatgt gttgttggag tatttggatt attattttga attgtcattt gttttccact
     3421 aagaatagtt aaaacgccac ttgcaaattt aaaatcgaca tttatttctc caccactaat
     3481 tttaaagtct cctacagatg taggatcttc tgcagtggct tcttccacaa ctaatgcatt
     3541 catatcttct tctgcatttc ctgcattaga atcttcttct gcatttcctg cattagcatc
     3601 ttcttctgca tttcctgcat tagcatctcc ttctgcattt cctgcattag aatcttcttc
     3661 tgcatttcct gcattagcat cccctcctgc atttcctgca ttagcatccc ctcctgcatt
     3721 tcctgcatta gcatcccctc ctgcatttcc tgcattagca tcccctcctg catttcctgc
     3781 attagcatcc cctcctgcat ttcctgcatt agtatctcct tctgcatttc ctgcattagt
     3841 atctccttct gcatttcctg cattagtatc tccttctgca tttcctgcat tagtactttc
     3901 ttctatagta tctgcgaaag tatttgacgc attaaatgat gctactgatc caagtcccat
     3961 tgagatactt aaaatcattg atagtgcttt ctttttacat ttttgttgca taaattcccc
     4021 ctccccttaa taatttttaa tatacactgt atacatcctt atatacactc taataattct
     4081 taatatacac tgtatacatc cttatatgca ctctaataat ttttaatata tacttaaaac
     4141 ttatatttgt caatgaatat actgtttttt tcaaccttat atattataag tattcattat
     4201 ttgctaaata actacaataa aaattttaat tatata
//
LOCUS       DAEPIN010000153         4225 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.156, whole genome shotgun sequence.
ACCESSION   DAEPIN010000153 DAEPIN010000000
VERSION     DAEPIN010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4225
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.156"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            complement(<1..489)
                     /locus_tag="KR250_003706"
     CDS             complement(<1..489)
                     /locus_tag="KR250_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069585.1"
                     /translation="MAYENGTDFIDYFPDCIEKYKEDNKIFEKAILLLNLYMQKPTWP
                     HCVNEYKSQAEKILGEDPEFIGKYGIQLRKFYDEDENAKVSKEFKNDLYNFSKSTYDI
                     LDNVYFSKSHPFNLSSIYSTSRDDDFIIKIFRYDNNFLELEMSKREVESLINYLSDLL
                     KEE"
     gene            complement(739..1551)
                     /locus_tag="KR250_003707"
     CDS             complement(739..1551)
                     /locus_tag="KR250_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBH0069586.1"
                     /translation="MKICITVGHSILKSGACTSADGVVNEYQYNKSLAPVLADTFRKE
                     GHKVDVIICPEKQFKTKSEEKSYKIPRVNAGGYDLLIELHLNASDGQGKGSEVLYYSN
                     KGLEYATRICKKLGTIFKNRGAKLDKGLYILNSSKPTAILIESFFCDNKDDYEKAKKF
                     GYEGMAKLIVEGVLNKNIGNDGVKLMYKHTIVYDGEVDKIPATVVGWGYNDGKILICD
                     IKDYIPGQTENLYIVGGGACNKIGSITKEKYTMIKGNDRFDTLYKALDFIDR"
     gene            complement(1551..1808)
                     /locus_tag="KR250_003708"
     CDS             complement(1551..1808)
                     /locus_tag="KR250_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HBH0069587.1"
                     /translation="MDNLISFIPEQLLILVAALSIIGKGCKKYKQLDNKYIPVVLLIL
                     GIGFSIWMLGLSPVAVLQGVICWGISIGINQTYKQLKEENK"
     gene            complement(1828..2058)
                     /locus_tag="KR250_003709"
     CDS             complement(1828..2058)
                     /locus_tag="KR250_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin XhlA family protein"
                     /protein_id="HBH0069588.1"
                     /translation="MNEELFKENLKRHEVTINKHNDEIDELKVANIESKAELKALCEN
                     LNSLTSMLKWLIGTMITTLVGFFIFAVQKGIF"
     gene            complement(2116..3681)
                     /locus_tag="KR250_003710"
     CDS             complement(2116..3681)
                     /locus_tag="KR250_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069589.1"
                     /translation="MATVYEFNYTGSEQSVTLKPGKYKFECWGARGGALGTPFESGFY
                     YGYGGYCSGEITLKKETTLYLYVGIDGRKGYNFNSAGYGNGASGGGATDIRLIGGTWD
                     NEQGLLSRIIVAGGGGGTYDKQHGGDGGGLKGTLGTSSTGAAAHGGTQFEGGRGRDKD
                     GSCDGFFGKGATPKNPSSQSGGGGGWFGGAYPASGFGNGSGGGSGYVLTKDSYKPPGY
                     TPTSEYYFDNVVMTTGGNTTVVGNYSDGRAKITLLQALPFLNISSYNSTTATFKADHT
                     DPTLLTKIEIFIDDILKETITTDLILEKTINYTLEDNALHTLKIVVTDSTNATAEKIV
                     SLSKNIMPLGDDASLQDIASKVSEIKEGLINGKTSIINVLALKNIESSLNNSLVELSE
                     KVKESFDSSDASLQDLINQLTQANNTISQLNSKYKYSSGTFTYYTGAYPNGLNIGFRP
                     DIISYTVINAVEEKYYCVYTPLFYLFYNYTTGEKYLDVNHNHYGITFTDNGWIPKGPV
                     NNGVYSYIAVKCC"
     gene            complement(3809..3946)
                     /locus_tag="KR250_003711"
     CDS             complement(3809..3946)
                     /locus_tag="KR250_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HBH0069590.1"
                     /translation="MNWYKIITDFYNNGNWTKEQVKTAVEKNKITATEYKEIVGEDYI
                     A"
     gene            complement(3947..>4225)
                     /locus_tag="KR250_003712"
     CDS             complement(3947..>4225)
                     /locus_tag="KR250_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069591.1"
                     /translation="METDEQNIKYIGMIHYKPTLLSEEELKNGILIEKLPVQQYVENK
                     EAKLFINIDTKEVFYRYTDIKSSIEDKVNSTEQTIADLTFQLMSNGVI"
BASE COUNT         1317 a          800 c          465 g         1643 t
ORIGIN      
        1 ttcttccttt aataaatcag ataaatagtt tattaatgac tctacttctc tctttgacat
       61 ctctaattca agaaaattat tatcatatct aaatattttt attataaaat catcatctct
      121 agaagttgaa taaatagatg aaagattaaa aggatgactt tttgaaaaat aaacattatc
      181 tagtatatca tatgtactct tagaaaaatt atacaaatca tttttaaatt ccttacttac
      241 ttttgcattt tcatcttcat cataaaattt tcttaattgt ataccatact ttcctataaa
      301 ctctgggtct tcacctaaaa ttttttccgc ctggctcttg tactcattaa cacaatgtgg
      361 ccatgttggt ttttgcatat acagattgag taataatatt gccttctcaa agattttatt
      421 atcttcctta tatttttcta tacaatcagg aaaataatct ataaaatcag ttccattttc
      481 ataagccatt ttaaatcccc ctaaaataat attatttaat attattctac attagaatta
      541 ttgttattgc actactattt tttatttcac ctccattatt ttatcaatat atatatttgt
      601 taactgttta tacacctagt tttttggatt ttaaaatggc ttatgattat ctttaatata
      661 tcatttttta ttttataaat tgtcgtaata ttggcataaa gtataagcaa aatgtaaaat
      721 acattaacta tactcctttt atctatcaat aaaatctaat gctttgtaaa gtgtatcaaa
      781 tctatcatta ccctttatca ttgtgtattt ttctttggtt atagaaccta tcttattaca
      841 tgctcctcca cccacaatat ataaattttc tgtctgacct ggtatataat cttttatatc
      901 acatatcagt atttttccat cattataacc ccagccaact acagttgcag ggattttgtc
      961 aacttctcca tcataaacga ttgtatgttt gtacatcagt ttaactccat catttcctat
     1021 attcttattc aatacacctt caacaattaa cttagccata ccttcatatc caaatttctt
     1081 agccttctca taatcatctt tattgtcaca aaagaaactt tcaattagta ttgcagtagg
     1141 ttttgaacta tttaaaatat ataatccttt atctaattta gcacctctgt ttttaaatat
     1201 tgtacctagt ttcttacata ttctagttgc atactctaga cctttattac tgtaatatag
     1261 aacctctgag ccttttcctt gcccatcact tgcatttaaa tgtagttcta tgagtaagtc
     1321 atatcctcca gcattaactc taggtatttt ataagacttt tcctcactct tagttttaaa
     1381 ctgcttttct gggcatatta ttacatctac cttatgccct tcttttctaa atgtatctgc
     1441 taatactggt gcaagagatt tgttgtattg atactcgtta actactccat cagcagaagt
     1501 acatgctcca ctttttaaaa tactgtgtcc tactgttata catattttca ttatttattt
     1561 tcctccttca actgtttgta agtttggttt atacctattg atatacccca acaaatcacg
     1621 ccttgtaaga ctgcaacagg acttagtcct aacatccaaa tagaaaatcc tataccaagt
     1681 attaataaca ctactggaat atatttgtta tctagttgct tatacttctt acagccctta
     1741 cctataatag agagagcagc tactaaaatt agcaactgct ctggtatgaa acttattaaa
     1801 ttatccattt tttatcctcc taattaatta aaatattcct ttctgaactg caaatataaa
     1861 gaaccctact agtgttgtaa tcattgttcc aattagccat ttgagcatac ttgtaagtga
     1921 gtttaaattc tcacacaatg cttttaactc tgctttagac tctatatttg ccacttttaa
     1981 ttcgtctatt tcatcattat gtttatttat tgttacttca tgtcgtttca aattttcttt
     2041 gaaaagttct tcattcatga aaacctcctt atttatatta aaataaggct tagaagttat
     2101 ctaagccttt gataattaac aacatttaac tgctatataa ctatacacac cattgttaac
     2161 tggaccttta ggtatccatc cattgtcagt aaaagttatt ccataatgat tatgattaac
     2221 atcaagatat ttttctccag ttgtataatt ataaaaaaga tagaataaag gtgtgtatac
     2281 acaataatat ttttcttcta cagcattgat aacagtgtat gaaataatat ctggcctaaa
     2341 acctatattt agcccattag gatacgcacc agtataataa gtaaaagttc cactagaata
     2401 tttatattta gagtttaact gtgatatagt attattagcc tgtgttaact ggtttattaa
     2461 atcctgcaaa ctagcatctg aactatcaaa ggattctttt accttctctg acaattctac
     2521 taaagaattg ttcaaacttg attcaatatt ctttaatgct aatacattta tgatagaagt
     2581 tttgccattt attaatcctt cttttatttc actaacttta cttgctatat cttgtagact
     2641 ggcatcatca cctaatggca ttatattctt acttagactt actatttttt ctgctgtagc
     2701 attagtactg tctgtaacaa ctattttaag agtgtgtagt gcattatctt ctaatgtgta
     2761 gttaattgtt ttttcaagaa ttaaatctgt tgtaatagtt tcttttaata tatcatctat
     2821 aaatatttct atttttgtta gtagtgtagg gtctgtgtgg tctgctttga atgttgctgt
     2881 agttgagttg tatgaggata tattcaaaaa tggtaatgct tgtaataatg ttattttagc
     2941 acgaccatct gagtaattgc ctacaacagt agtatttcca ccagttgtca tgactacatt
     3001 atcaaaataa tattcagaag ttggtgtata tccaggtggc ttataactat ctttagttag
     3061 tacataacca cttccaccgc cacttccatt accaaatcca ctagcaggat aagcaccacc
     3121 aaaccaacca ccgccaccac cagattgtga tgaagggttt ttaggagtag cacctttacc
     3181 aaaaaaacca tcacaagaac catctttgtc tctacctcgc ccaccctcaa attgtgtacc
     3241 accatgagca gcagcaccag tagaagaagt tccaagagtc cctttcaagc caccaccatc
     3301 accaccatgt tgtttatcat aagttccacc gccaccacct gcaacaatta tacgagatag
     3361 taaaccttgt tcattatccc aagtaccacc aataagccta atatcggtag caccaccacc
     3421 acttgcacca ttaccatatc cagcactatt gaaattataa ccttttctac catctattcc
     3481 aacataaaga tataaagtag tttctttttt taatgttatt tcaccactac aatatccacc
     3541 ataaccataa taaaaaccac tttcgaaagg agttcctaat gcaccacccc tagcacccca
     3601 acattcaaat ttgtatttac ctggtttcaa tgtaacactt tgttcacttc ccgtataatt
     3661 aaattcataa actgttgcca cttaatccac ctctttctta atttatggtt ttattgtacc
     3721 taattttaaa actacaaacg tctacaaagt gtagatgaag tgtaggcaaa atgtaaatga
     3781 atttatatat tttaagaaat aaaaaagact atgctatata gtcctctcct acaatttctt
     3841 tatattctgt tgccgttatc ttattctttt ctactgccgt tttaacttgc tctttagtcc
     3901 aattaccatt attatagaaa tctgttatta tcttgtacca attcatttat atcaccccat
     3961 ttgacattaa ttgaaatgtt aaatctgcta ttgtttgttc tgtagaattt actttatctt
     4021 ctatgctact tttaatatct gtatatctat agaaaacctc tttagtatct atatttataa
     4081 ataactttgc ttctttattt tctacatatt gttgtactgg caatttctca attaaaattc
     4141 cattttttaa ctcttcttct gatagtaaag ttggtttata gtgtatcatc ccaatatatt
     4201 ttatattttg ttcatctgtc tccat
//
LOCUS       DAEPIN010000154         4153 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.157, whole genome shotgun sequence.
ACCESSION   DAEPIN010000154 DAEPIN010000000
VERSION     DAEPIN010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4153
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.157"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            complement(<1..1231)
                     /locus_tag="KR250_003713"
     CDS             complement(<1..1231)
                     /locus_tag="KR250_003713"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069592.1"
                     /translation="MTFKELVNKVRNLVLEAKNVTIEDTENNFTSDNVEGALKEVFQN
                     GVNAKNNVVTALNSKGAEVTTSDTWEEIKNKIDIKEGRLDLRETILANSYSSYLVTNG
                     AIKYIEKYSGSLKALEYEEPYFYAIKGTHLIKINAIDESVIFDITLANANFSCICVTQ
                     EYLFISDNTKLYKINKLTGNEAQSIEGSYYKLCTYGEFIYGIYGDETSSILHKIRISD
                     MYIMLTKDMSSDRIYDFERGKFVCNNNGIYATTEHSNSSGLTECYLTKINFDFSVAKS
                     FRIGGYLYEKNIKFLNDFVFVSDSSRSIEVESGKKSGLAKYDANLNLITYGGSDRYEN
                     FEIYNGYIYTFNSLSSSPFIKINLNTLKEVNSYRTLINTNPQKGMFIINNIIFFIGGG
                     IHRNILSKKVYSDEKGEE"
     gene            complement(1265..1447)
                     /locus_tag="KR250_003714"
     CDS             complement(1265..1447)
                     /locus_tag="KR250_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069593.1"
                     /translation="MNINNVVVRILAERILNGGLNPLKNREFQLDDVTNTEYRKAVED
                     YIIKNSGVVEGIEPTA"
     gene            complement(1447..1659)
                     /locus_tag="KR250_003715"
     CDS             complement(1447..1659)
                     /locus_tag="KR250_003715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069594.1"
                     /translation="MKKQVYYNSLDEKEKIISENSNLYVIEIYETLNENYLVLSSSPI
                     QDEKLSYEELENELLIMTNELQGGLL"
     gene            complement(1669..2307)
                     /locus_tag="KR250_003716"
     CDS             complement(1669..2307)
                     /locus_tag="KR250_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069595.1"
                     /translation="MTEKLTENASLGELMAALENVQTDFQTGKNNISSALGSPFIGTD
                     KFGTTKTKIETLKNVLVETINSKNVSATSSETFTNLIEKVNWIFQSIEIFSLKNRIQA
                     TTLNTPSIVYNEVSSIKGTLRFTGELKASKMRADYATAKIEILCGNRKEYFYVTDDTP
                     SASSSFVRFTKDIIVENGMDIKVQILLTSVGAGNLDGSYAARAEIEKLTILR"
     gene            complement(2321..3430)
                     /locus_tag="KR250_003717"
     CDS             complement(2321..3430)
                     /locus_tag="KR250_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBH0069596.1"
                     /translation="MAIDKSYYTIITDVGKAKIANASVTGNKVGFVKIQLGDGGGSEY
                     TPTESQTALKNVVWEGNIGNTTTDETAPNCIILESLIPSSVGGFMIREIGYLDDENNL
                     IAISKYKECYKPSIEQGAVVDMKVKTVLIVSNVNNIELKIDPTIIFATLKDIQDLETK
                     IGTVNTKIDTTKTELTNNLETAKTELNTRIDTENEKQNIKIDQLIAGGSNVASTQTIT
                     IDDWVEDAENGFKATVTHSLLTQRIVVNIIDATTKENAVTNFKIIDDNSIEVRSETRS
                     ELNVYVINGNAETHFINATVDDNRVSEMTTYSSKKIEDSISSIQLIDTSISITDANDR
                     FTSDKLDGVLEEIMVEISGQRTKGITIVNNLIDMI"
     gene            complement(3442..4059)
                     /locus_tag="KR250_003718"
     CDS             complement(3442..4059)
                     /locus_tag="KR250_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HBH0069597.1"
                     /translation="MDKEINLINYLPQILQDKEEYIKVFNVGNKEIKILHDKLKDLSN
                     DQFLEDLTISGIKRWEKIMSITPKSNESLEDRRFRIFSKYISKLPYSERFLRNWLDSI
                     VGEGNYELTINNATYNIHLESDARNQDWFEEVHSFVSNIKPCNMTLDYTRVLISKDNY
                     MNFGITTLMGQEITIYPWSPPDIETYGEIDVLTGNGVGYQEITIF"
BASE COUNT         1257 a          736 c          424 g         1736 t
ORIGIN      
        1 attcctctcc tttttcatct gaataaacct tttttgataa tatatttctg tgaatgccac
       61 caccaataaa aaaaattata ttgtttatta taaacatacc tttttgagga tttgtgttaa
      121 tgagcgttcg gtagctatta acttctttaa gagtatttaa atttattttt ataaatgggc
      181 tactagaaag tgaattaaat gtatatatat atccattata tatttcaaaa ttttcatatc
      241 tatctgaccc accataagta atcaaattta gatttgcatc atattttgct aatccacttt
      301 ttttaccact ctcaacttct atgcttctgc tactatcaga tacaaaaaca aaatcattta
      361 aaaacttaat gtttttttca tataaatatc ctccaattct aaaacttttc gcaacactaa
      421 aatcaaaatt tattttagtt aggtaacatt ctgtaagacc acttgaattt gagtgttctg
      481 ttgtagcata aataccatta ttattacata caaatttgcc tctttcaaag tcataaattc
      541 tatcagagga catatcttta gttagcatta tatacatatc cgatattctt attttgtgaa
      601 gtattgaaga agtctcatct ccatatattc cataaataaa ttccccataa gtacatagct
      661 tataataaga accttctatt gactgtgctt catttcctgt taacttattt attttatata
      721 atttagtatt gtcagatata aataaatact cttgagtaac acagatacat gagaagttag
      781 cattggctaa agtaatatca aaaattacag attcatcaat tgcattaatt ttaattaaat
      841 gtgtaccttt aattgcataa aaatagggtt cttcatattc taatgctttt aaactcccac
      901 tatatttttc aatatatttt atagcaccat ttgtaaccaa atatgaagaa taactatttg
      961 caagtattgt ttctcttaaa tctaatctcc cctcttttat atcaatttta ttctttattt
     1021 cttcccatgt atcgcttgta gtaacctctg ctcccttgga gtttaatgct gttactacat
     1081 tatttttagc attaactcca ttttgaaaaa cctcttttaa tgctccttct acattatcac
     1141 ttgtaaagtt attctctgta tcttctatag ttacattctt tgcttctaat acaagatttc
     1201 taactttatt aactaactct ttaaaagtca tttagtcacc ttctttcaat aaaaaaagaa
     1261 cctactacgc tgttggttct attccttcta ctactccact attttttata atataatcct
     1321 ctactgcttt tctgtattct gtgttagtta catcatcaag ttgaaactct ctatttttca
     1381 aagggtttaa ccctccattt aatattcttt ctgctaatat tcttaccaca acattattta
     1441 tattcattat aacaatcctc cttgtaattc attagtcata attaataatt cattttctaa
     1501 ttcttcataa ctaagttttt catcttgtat tggactactt gataacacta aataattttc
     1561 atttaaagtt tcatatattt ctataacata taaattagag ttttcactaa ttattttttc
     1621 tttttcatct aaagaattat aatatacttg ttttttcata atttattcct accttaatat
     1681 tgttaatttt tctatttctg cacgagctgc atatgaacca tctaaatttc ctgctcctac
     1741 agatgttaat aatatttgta cttttatatc catgccattc tcaacaataa tatcttttgt
     1801 aaatcttaca aaagaagaag atgcactagg agtatcgtca gtaacataaa agtattcttt
     1861 tcggtttcca catagtatct ctatttttgc tgttgcataa tcagcacgca ttttagacgc
     1921 tttaagttcg cctgtgaatc ttaatgtgcc ttttatacta gatacttcat tgtaaacgat
     1981 gctaggagtg tttaaagttg tagcttgaat tctatttttt aaagaaaaaa tttctattga
     2041 ttgaaaaatc cagttaactt tttcaatcaa attagtaaat gtttcagatg atgttgctga
     2101 aacattttta gaattaatcg tctccactaa tacatttttt aatgtttcta tttttgtttt
     2161 agttgtacca aatttatctg ttccaataaa aggactaccc aatgcactag atatattatt
     2221 tttaccagtt tgaaaatcag tttgtacatt ttctaatgct gccataagtt cccctaaact
     2281 agcattttca gttaattttt ctgtcatatt ttcacctcgc ttatatcata tctattaaat
     2341 tattcactat agttattcct ttagttcttt gaccacttat ttctaccatt atttcctcta
     2401 atactccatc taacttatca cttgtaaatc tatcattagc atctgtaata cttatactgg
     2461 tatctataag ctgtatactg ctaatagaat cctctatttt cttagatgaa taagtagtca
     2521 tttcagacac tctgttatca tctacagttg cattaataaa atgagtttct gcatttccat
     2581 ttatcacata aacgtttaat tctgaccttg tttcacttct aacttctata gaattatcat
     2641 ctataatttt aaagtttgta actgcatttt cttttgtagt agcatctata atatttacaa
     2701 ctattctctg tgttaataaa ctatgtgtta cagttgcttt gaatccattt tctgcatcct
     2761 caacccaatc gtcaattgtt attgtttgag tagatgccac attagagcca cctgcaataa
     2821 gttggtcaat tttaatattt tgtttctcat tttctgtgtc aattctagta tttaactctg
     2881 ttttagcagt ttctaaatta tttgttaatt ctgttttagt tgtatctatt ttagtattaa
     2941 cagtacctat tttagtttct aagtcttgta tatctttgag tgttgcaaag attattgttg
     3001 ggtcaatttt aagttctata ttatttacat tagatacaat aagcacagtt ttaaccttca
     3061 tgtctaccac tgcaccttgt tctatagaag gtttataaca ctctttgtat ttagaaatgg
     3121 caattaaatt attttcatca tctaaatatc ctatttctct tatcataaat ccgcctacac
     3181 ttgatggtat taaactctct aatattatac aatttggtgc agtttcatct gtagttgtat
     3241 ttccaatatt gccttcccat accacgtttt tgagagctgt ctgactctca gttggagtat
     3301 attcactccc tcctccatca ccaagttgaa tttttacaaa tcccacttta ttacctgtga
     3361 cacttgcatt tgctatcttt gcttttccta catctgtaat tatagtgtaa taacttttat
     3421 ctatagccaa tatatcacct cctaaaatat tgttatctct tggtatccaa ctccattgcc
     3481 agttaataca tcaatttctc cataagtttc tatatctggt ggactccaag ggtatatagt
     3541 tatttcttga cccattaggg ttgttatacc aaaattcata taattgtctt tgcttataag
     3601 cactctagtg taatctaaag tcatattaca tggcttaata ttacttacaa aagaatgaac
     3661 ttcctcaaac caatcttgat ttctagcatc actttcaaga tgtatattat aagtagcatt
     3721 attaatagtt aactcataat taccttctcc aactatacta tctagccaat tccttaaaaa
     3781 tctctctgag taaggtaatt tacttatata tttactaaaa atcctaaacc ttctatcttc
     3841 taaactctca ttacttttag gagttataga cattatcttt tcccatcttt ttataccact
     3901 tatagttagg tcctctaaaa actggtcatt tgataggtcc tttaatttat catgtaatat
     3961 ttttatttct ttatttccta cattaaatac ttttatatat tcttctttat cttgtagaat
     4021 ttgtggtaag taatttatta gattaatctc tttatccaac tacctcacct ctcactacta
     4081 tactgttact atttattgtt agattagatt taacctcatt tatcattgta tttgcaatgt
     4141 ctaatactcc atc
//
LOCUS       DAEPIN010000155         4022 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.158, whole genome shotgun sequence.
ACCESSION   DAEPIN010000155 DAEPIN010000000
VERSION     DAEPIN010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4022
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.158"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            complement(255..791)
                     /locus_tag="KR250_003719"
     CDS             complement(255..791)
                     /locus_tag="KR250_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HBH0069598.1"
                     /translation="MEYKSPVYGIIAVPIEKIESNDYNPNAVAPPEMQLLYKSIKEDG
                     YTMPIVCYYNEEKDKYIIVDGFHRYRVMLENEDIYDRENGLLPVSVIDKSLDERMAST
                     IRHNRARGSHNVDLMSKIITELCEIGKSDEWISKHLGMEVDEILRLKQITGLAYLFKD
                     KEFGNAWIPFGSDIEEEN"
     gene            complement(809..2122)
                     /locus_tag="KR250_003720"
     CDS             complement(809..2122)
                     /locus_tag="KR250_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HBH0069599.1"
                     /translation="MDKRYLESDVFEAFQERLSYLFNEFDYIYVSFSGGKDSGLLLNL
                     VLDYKRKNNISSKIGVFHQDFEAQYSLTTEYVEKVFEDNIEDIEPYWVCLPMATRTAV
                     GNYEMYWYPWDDEKEELWVREMPVKEYIINQKNNPFTYYKYKMRQESLAKQFGRWIRD
                     IHEGAKVVCLLGTRAAESLQRYCSIVNKQHGYKGKCWITKEFKNVWTASPIYDWEIED
                     IWLAHYKFDYDYNKIYDLFYKAGVAPRKMRVASPFNDSAKESLNLYRILDPDVWVKLV
                     ARVQGVNFTSIYGKTKAMGYRSVKLPYGHTWKSFTYFLLDTLPENIRNSYIKKFSTSI
                     EFWQNTGGGLSEIVIRELLRKGYKIELNGVSNHTKDKKSRVIFLDDIPDDTDDIKFTK
                     DIPSWKRMCYCILKNDHMCRFMGFGPTKEQRIRTEYIKKKYEKIH"
     gene            2477..2917
                     /locus_tag="KR250_003721"
     CDS             2477..2917
                     /locus_tag="KR250_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HBH0069600.1"
                     /translation="MYERMLDKSNKPCLSQIIEYIGKDGYARLCKLETYLKTQYNLSR
                     ELRFPFGNNYGWGYKYSHKSFHLFYAFFEKGAFTVLIQIGDKQVPAVEKTLVALTPKA
                     KELWVTRYPCGNNGGWIKYRILEDADLLDIIQYIRAKKSPPKQN"
     gene            complement(3102..3290)
                     /locus_tag="KR250_003722"
     CDS             complement(3102..3290)
                     /locus_tag="KR250_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069601.1"
                     /translation="MNKNNNLVIICMFIGMILGMAIGCAIGISKGNVGITMCYGLIFG
                     MIIGICIGTVIKNSNKKE"
BASE COUNT         1412 a          667 c          384 g         1559 t
ORIGIN      
        1 atgtgattat tttttattaa aaacgatata aatatacaat ttaatactat ttttttataa
       61 ataaacttgt attaaaatac atttagttat ataattttat tttttctaag agtataatta
      121 taaaaataaa aaaagaatag gataattttc ctactctttt ttctgtactt acatataata
      181 ttaaaactct ttatttttta atatatattt taaattttct tttttaataa atctttttct
      241 tttaataaat ctatttaatt ttcttcttct atatcagagc caaagggaat ccatgcatta
      301 ccaaactctt tatctttaaa taaataagct aatccagtta tttgcttcaa tctaagaatt
      361 tcatcaactt ccatacccag gtgctttgat atccattcat cagattttcc aatttcacaa
      421 agttcagtta taattttact cattaaatcc acattatgag aacctcttgc acgattgtgt
      481 cgaatagtac ttgccattct ttcatcaaga gatttatcaa ttactgaaac aggaagaagt
      541 ccattttctc tatcatatat atcctcattt tcaagcatta ctctatatct atgaaaacca
      601 tctacaataa tatacttatc cttttcctca ttataataac agacaattgg cattgtatac
      661 ccatcttctt ttatactttt atacaacaac tgcatttctg gtggggctac tgcatttgga
      721 ttatagtcgt tagattctat cttttcgatt ggcactgcga tgattccata tacaggactt
      781 ttatattcca tagcatctta actcctatct aatgaatttt ttcatatttc ttcttaatat
      841 attccgttct aattctctgt tcttttgtag gaccaaatcc cataaatcta cacatatggt
      901 cattttttaa aatacaataa cacattcttt tccaacttgg tatatctttg gtaaatttaa
      961 tatcatccgt atcatcaggt atatcatcca aaaatatgac tctactcttt ttatcttttg
     1021 tatgatttga aactccattt aattctattt tataaccttt tctcaacaat tcacgtataa
     1081 ctatttctga caaaccacca cctgtattct gccaaaactc aatagatgtt gaaaattttt
     1141 taatatagct attccttata ttttcaggta aggtatcaag caaaaaatat gtgaaacttt
     1201 tccatgtgtg tccatatggc aatttaacac tacgatatcc catagctttt gttttgccat
     1261 aaatagaagt aaaattaact ccttgtactc tagcaaccaa tttcacccat acatctgggt
     1321 ccaatattct gtataaattt aaactctctt ttgctgaatc attaaaaggt gatgccactc
     1381 tcatttttct tggagctact ccagctttat agaataagtc ataaatttta ttatagtcat
     1441 agtcaaattt atagtgtgct aaccatatat cttctatttc ccaatcatat ataggagatg
     1501 ccgtccagac atttttaaat tccttagtta tccagcactt tcctttatat ccatgctgtt
     1561 tattcacaat agaacaataa cgttgtagag attctgctgc tcgtgttcct aataagcaaa
     1621 caacttttgc accttcatgt atatctctaa tccatctacc aaattgtttt gccaaacttt
     1681 cttgtctcat tttatattta taataagtaa aaggattatt tttttgattt attatgtatt
     1741 ctttgactgg catttccctt acccataact cttctttctc atcatcccat ggataccaat
     1801 acatttcata atttcctaca gcagttcttg tagccattgg taaacacacc caatatggtt
     1861 caatatcttc aatattatct tcaaatactt tttcaacata ttcagtagtc aaactatatt
     1921 gagcctcaaa atcttgatga aatactccta ttttggaact tatattattc tttctcttat
     1981 aatccaaaac taaatttagc agtaaaccac tatcttttcc tccgctaaaa gatacataga
     2041 tataatcaaa ttcattaaaa agataagaaa gtctttcttg aaaagcttca aatacatcgg
     2101 attctaaata tctcttatcc atttgaatta tcccctttca attagaagtt tataaaacct
     2161 tacaatgcaa tattattatg cttttttagc agtaatcagc ataaattata cattatttaa
     2221 atacacaaat ccaactaact acatataatt ataatgtaat tatacttttg tattataaac
     2281 tatattttat cattatttta catttttttc caatatatat aaatatcata tttttctatt
     2341 agctaaaact aattacttta tatttctacg tattagtata ataatacata aaaacttgaa
     2401 taatgcataa aaacttgaca tactgttgtc ataattactt tgctataatt aaaagtaaaa
     2461 atggaggtaa attctaatgt atgaacgtat gcttgataaa agcaataaac cctgtttgtc
     2521 tcaaataata gaatatatag gtaaagatgg atatgcacga ttgtgtaagt tggaaactta
     2581 cttgaaaact caatataatt tatcaagaga acttcgattt ccttttggaa acaattatgg
     2641 ttggggttac aaatatagcc ataaatcttt tcatttattt tatgcttttt ttgaaaaagg
     2701 tgcatttact gttctgattc agataggtga caagcaagtt ccagcagtag aaaaaacgtt
     2761 ggtagctttg acaccaaaag ctaaagagct ctgggtcact cgttatcctt gtggcaataa
     2821 tggaggctgg attaagtatc gtattttaga agatgcagac ttacttgata ttattcaata
     2881 tataagagca aaaaaatctc cacctaaaca aaattaactt ttattttttg tgatttaaat
     2941 atagtgtata taaattccat tctttccata taaaaaaaat aatataaaaa atatctatta
     3001 gtataactta tttagtatgt cttattctac tttttacaga taattaaaaa gttttatcat
     3061 aaataaatca taactagaca tcctagtaaa tcaaaattaa tttattcttt tttattagaa
     3121 tttttgataa cagtccctat acatatgcca attatcattc caaaaattag accataacac
     3181 attgtgatac ctacatttcc tttagaaata cctatagcac aacctatagc cattccaagt
     3241 atcatgccta taaacataca tataataact aaattgttgt ttttattcat agttttctcc
     3301 attcaatata ttttaaattg cctagctcgc cttcttagct taaaacaaag aaatataaat
     3361 taactttctt tcattctaat atctgcttgt ataaatgtca aacaacacat ttcttgtcat
     3421 aacctcctct ttttgaagta agtacaatct cccagacgca ataatatcaa tttcctaaaa
     3481 ccatttaagt aacttttata tttacataat atttacaatc ataaactata tttttattca
     3541 tttatacatc ataaacactc cctactaatc attattatac ttaaaataat gttcatttat
     3601 aataatatat ctaacacact ttaattgaaa aaaataaaac tttgcctttc attcccatat
     3661 ataaataagt atactttttg atgctctatt acaaatcaac taaattaatt gatataaatt
     3721 atcattttct gttgaaaata ccattcgcac atgctatact taattgcata aatggtaata
     3781 aatgtcatat gattttacaa atctattaat aacaattatt acttttactg tattttaaat
     3841 tttttggact aaatatcaag aactttctaa aatacaaagt acaaaaaatt tatataagtt
     3901 ataaatttat atgaactaga taagctcaaa caatatgtta tgtataaact gtgtagtatt
     3961 cggttataaa tttatataaa ttatataagc tagataaagt attacctcaa ctatataagc
     4021 ta
//
LOCUS       DAEPIN010000156         3901 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.159, whole genome shotgun sequence.
ACCESSION   DAEPIN010000156 DAEPIN010000000
VERSION     DAEPIN010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3901
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.159"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            complement(<1..1820)
                     /locus_tag="KR250_003723"
     CDS             complement(<1..1820)
                     /locus_tag="KR250_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HBH0069602.1"
                     /translation="MATIQTSIKIFDGMTPAFRNMTNSINTTINSLERLQGRLNNPLN
                     AGNIQASQQSLNNIESILTRIEQKIGRNTNEQENFNNKIRQGSEAGSLLVSKLKSIAG
                     IYIGIKGIDSITKVADTIASTKARLNLMNDGLQTTDQLNKMIYLSAQSARASYADTAA
                     QIAKLGILAGDAFGSSAEVIKFTELMNKAFVIGGTSANEASAAMYQLTQAMGAGKLQG
                     DEFRSIMENAPLLATKIADAMGKTKDQLKELSSSGAITADVIRNALFKASDEIEKKFA
                     SMAITFSQALTMMKNDAYMIFSETLSKISGALQSVRFSEIVVSIRNVMIAISSNIYDT
                     LNIIKNILNSDFFSDFVSNVTLGAILIINGLGWVTNATLNVANVFAQNWSIIAPIIYG
                     VIAAIAIYKGVLLASTIATTVASFVNSLYAVAAYKSCAALAAQEFAIFGKISAQTMEA
                     LVTAQATAAQWGFNAALLSCPIFWIIAGIIAFVVVVFVAVAAVNKFSGTSLTVLGAIV
                     GAVFAAVAFIQNIMIWLFNRCVDVNEGIANGWNQCVYLMKQAIAKGVIFIIEKMASLN
                     DAVNNAGNALGKAFIDGANIAIRGVNKLIDLINKIPGINIG"
     gene            complement(1885..2400)
                     /locus_tag="KR250_003724"
     CDS             complement(1885..2400)
                     /locus_tag="KR250_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBH0069603.1"
                     /translation="MFCSNCGYEITGAGKFCSNCGTATLADKVNNDDLFINVHGKELN
                     LTNIYKETKGDKILAIDIAMKLLGLDIKECKNIIYPAFKELSEKINIEEEKEILREDE
                     YKQTNVLEDDVARCPRCGSVSLSAHKKGFGIGKAVAGATIAGGIGLVAGNLGAKKVRV
                     TCLSCGKQFWA"
     repeat_region   2530..3481
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=2728..2756
                     /rpt_unit_seq="atttacataccacatagttaatataaaac"
BASE COUNT         1301 a          746 c          447 g         1407 t
ORIGIN      
        1 ccaatattta tccctggtat tttatttatt aggtcaatta atttgtttac acctcgtatt
       61 gcgatatttg ccccatctat gaaagctttc ccaagtgcat ttccagcatt atttacagca
      121 tcatttaatg atgccatttt ctcaattata aagattacac cttttgcaat agcttgtttc
      181 attagatata cacattgatt ccatccattt gcaatccctt catttacatc tacgcatcta
      241 ttaaatagcc atatcattat gttttgtata aatgctactg ctgcaaatac tgcacctaca
      301 attgctccta gtacagtcag acttgttcct gagaatttgt ttactgctgc tactgcaaca
      361 aaaactacta ctacaaatgc tataatacct gctattatcc aaaaaattgg acaggataat
      421 aatgctgcat taaaacccca ttgtgctgct gttgcttgtg ctgttaccaa agcttccatc
      481 gtttgtgcag atatttttcc aaatatcgca aattcttgtg ctgctaaagc tgcacaagat
      541 ttatacgctg ctactgcata caatgaattt acaaaagatg ctaccgtagt ggcaattgta
      601 cttgcaagta atactccttt atatatcgca attgctgcta tcactccata aataatagga
      661 gcaataatcg accaattttg agcaaagaca ttagcaacat ttagtgttgc attagtaacc
      721 catcccagtc cattgataat tagtatagct cctaatgtaa cattgctaac aaaatctgaa
      781 aaaaaatcac tatttaatat attttttata atatttaatg tatcataaat gtttgaagat
      841 attgcaatca taacattccg gatagataca acaatctcac taaaacgtac actttgcaaa
      901 gctccactta tcttactgag agtctcagaa aatatcatat aagcatcatt tttcatcatt
      961 gtgagagctt gagaaaaagt gattgccata cttgcaaatt tcttttctat ctcatcagaa
     1021 gctttaaaca gtgcatttct tataacatct gctgttattg ctccgctact tgataattcc
     1081 ttcaattggt ctttagtttt tcccattgca tcagctattt tagtggctaa taaaggtgca
     1141 ttttccatta tggaacggaa ctcatcacct tgaagttttc ctgcacccat ggcttgtgtt
     1201 aattgataca ttgctgcact agcttcattt gctgatgttc ctccaattac aaaagcttta
     1261 ttcataagtt ctgtaaactt tatcacctct gctgaacttc caaaagcatc tcctgcaagt
     1321 attccaagtt tagcaatctg tgctgctgta tctgcataac tagctcttgc actttgggct
     1381 gacaaataaa tcattttatt aagctggtct gttgtctgta agccatcatt cattagattt
     1441 aaacgtgctt ttgtacttgc aattgtatct gctacttttg taatactatc tattcctttt
     1501 attccaatgt atatcccagc aatacttttt aatttagaca ctaatagaga acctgcttca
     1561 ctaccttgtc ttattttatt attaaagttt tcctgttcat ttgtatttct tccaattttc
     1621 tgttctatcc ttgtgagaat actttctatg ttattcaaac tttgttgaga agcttgtata
     1681 ttacctgcat tgagtggatt attcaatctg ccttgcaatc tctctaaact attaattgtt
     1741 gtattaatag aattagtcat attacgaaat gctggtgtca ttccgtcgaa aatctttatt
     1801 gaagtttgta ttgtagccat ttcctcactc tcctttcata aatttccata taaaaaaaca
     1861 cctacattag taagtgttta ttatttatgc ccaaaactgt ttaccacagc tcaaacatgt
     1921 aactctaact ttctttgctc ctaaatttcc agctactaaa ccaatgcctc ctgctatagt
     1981 agcccctgct acagcttttc ctatgccaaa acctttctta tgagcagaca atgaaacaga
     2041 gccacaccta gggcaacgag caacatcatc ttcaagtaca ttagtttgtt tatactcatc
     2101 ttccctcaat atctcttttt cctcttcgat atttattttt tcacttaatt ctttaaaagc
     2161 tggatatata atatttttac actcttttat gtccagccct agtaacttca ttgcaatatc
     2221 aattgctaag attttatctc ctttagtttc tttataaata ttagtcagat ttaattcttt
     2281 tccatgaaca tttataaata aatcatcatt gttaacttta tctgctaatg tagctgttcc
     2341 gcaatttgag caaaatttgc ctgcaccagt tatctcataa ccacaatttg aacaaaacac
     2401 aacaatcacc tcacataata cttattttat agcactatta tactatatac gtaaaatttt
     2461 tacattatta tcacctcctt tcattaaaaa aacacttact catttgtaag tgtttttgaa
     2521 ttatttttaa ttttaagtcc acatagttaa tataaaattg tttgctcaga tgcttttaaa
     2581 tactcaaaca ttagccttta cataccactt agttaatata aaccaaacca atgcacaatt
     2641 tgaaaaaatg ccttttacac tatttacata ccacttagtt aatataaaac gcgttaatgc
     2701 agtaccataa cgatatggga ctaactaatt tacataccac atagttaata taaaacagta
     2761 ttcagaactt gaaaaaaaag ttggaaatag cacatttaca taccacatag ttaatataaa
     2821 accgtatagg aatgaatgca ggtagtgcat tttctagtac atttacatac cacatagtta
     2881 atataaaaca agtagcagga gaagctaaga taaaagttga acgaaattta cataccacat
     2941 agttaatata aaacttgcag ataatactct aagacttgaa gttaaatgtg catttacata
     3001 ccacatagtt aatataaaac cattagaaga atataacata gtagaagatg cagaaaaatt
     3061 tacataccac atagttaata taaaacacca gaaaaggaag gtggcgatat tcttataaca
     3121 aaatttacat accacatagt taatataaaa ccgatatcca tttctccttt tccagtaata
     3181 ttaaatttat ttacatacca catagttaat ataaaacccc aatcatacga accaggaaca
     3241 tatggcaaac tataatttac ataccacata gttaatataa aacaaagact atatcctatg
     3301 ttcataattg ggagtaatac atttacatac cacatagtta atataaaaca attagatgct
     3361 tttgttaaag aacatgcagt tttcaaattt acataccaca tagttaatat aaaacaattt
     3421 tagtattgta attgacgtgc ttttttaaaa agatttacat accacatagt taatataaaa
     3481 ccccaaaata aattgtgtat ttccaagact tacacacata taactttctc aaatttgcag
     3541 tgagcgggta atagtgcaat tgataacact tatcaaacct tctcaatgcc ttgtattcca
     3601 attgttaaac tatacttaag tagaaaaatc gaacactgca aaattcctat atttttatta
     3661 taccatttta taccaaataa agcacttgaa acaacataat atccaagtgc tttatctatt
     3721 tatttaatta tttttctcta attttttctc agaaagttta tatactattc tttctatctt
     3781 attatatata tatatctata tctgtttctc ttattttcaa aagtttaatt ctaaattctt
     3841 cttttctctt ttcaatatct atactatcaa gtaaattctc atacattgta ttaccccccc
     3901 a
//
LOCUS       DAEPIN010000157         3765 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.160, whole genome shotgun sequence.
ACCESSION   DAEPIN010000157 DAEPIN010000000
VERSION     DAEPIN010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3765
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.160"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            <1..453
                     /locus_tag="KR250_003725"
     CDS             <1..453
                     /locus_tag="KR250_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2953 domain-containing protein"
                     /protein_id="HBH0069604.1"
                     /translation="NRQLYPAENSKNNDKKEGMKNNIDSSILLADDLLSIYRLLKKIK
                     IHELYSNINFGTGNIGLTSSVYVLINTLYGNLFNMIDAEKMYLNVNPDFTKDYVLGNI
                     RIHIRPRIKALFNIIIMINKIMNKNKGNKEGDSNESNRFDTESYGNNS"
     gene            407..835
                     /gene="ytfJ"
                     /locus_tag="KR250_003726"
     CDS             407..835
                     /gene="ytfJ"
                     /locus_tag="KR250_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerW family sporulation protein"
                     /protein_id="HBH0069605.1"
                     /translation="MRATGSIQSLMETTLETIKGSIDANTIIGDPIKTDTTVVVPISK
                     VTIGFGIGGGEYSKGYDDKDREIELKNEKSDTNFAGGSAGAISVQPVAFVVVESGETR
                     IMSLDSNINLVDNILSITPRVLEKIQNISQSNKNQDKNNM"
     gene            1083..1523
                     /locus_tag="KR250_003727"
     CDS             1083..1523
                     /locus_tag="KR250_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBH0069606.1"
                     /translation="MSDKELSILLLESMNNFHNLIKIINNERYKKDKVLTERQFFALV
                     KIRKHDKIELKNLSRDLHVSTSSLCILLNKLVEQEYVYREEDSRDRRNTFYGITKNGE
                     KILDNEILKFVSIISDKMDCLDIDNKDKLFTSLEESKNIIEQLF"
     gene            1539..2990
                     /locus_tag="KR250_003728"
     CDS             1539..2990
                     /locus_tag="KR250_003728"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HBH0069607.1"
                     /translation="MNIKYSKEILKSLENAITVVALFEGEDVSTTFDKINMISRNFNS
                     KENSIDTVTLIENNNLVNVCLVGFGKRELLNREKIRIIGGNLCKKLKNILKNEAYKSS
                     DVNVLNLNLNSDEIGSFVEGILLGNYKFDKYKTKIKDFERKEVDTITIFTEKEVECQI
                     EKAKILANSTIIARNLVNEPSNVIYPKTLAVETVKLGAEFGFNVDVYEEEKIKALGMD
                     AFFAVSRGSINKPRFIVMRYFGDKESTDILGLVGKGLTYDTGGYSLKSNASMLDMKTD
                     MAGAASVIGAMCAISQSKLKKNVIAVVAACENALSGGSYKPGDIISSMAKKTIEVLNT
                     DAEGRLTLADAIYYIINNEKVTKVVDVATLTGAALTLLGNVATPIVTNNDDFYCELEK
                     AATLSGERVWKMPIYDEFKDMIKGEEADLKNTGGKNAGCITAGAFIGEFVGNTPWIHM
                     DIAGTSTSSKSIGYKAKGATGEPVRTLYYLAEI"
     gene            complement(3081..3749)
                     /locus_tag="KR250_003729"
     CDS             complement(3081..3749)
                     /locus_tag="KR250_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwaF family protein"
                     /protein_id="HBH0069608.1"
                     /translation="MFKIFLFSSEQFVSLFIFGLFLYYCPKLTKNILPYSYTVEKIIC
                     TLLVIIMALEQLLLISSGNYSTLNSLPIGINYICIYLCIAILIFKQYHLFNIFFSWSL
                     VCSVGELIFSKNLGYEFPSLIYFIFIFSKCLIIYADIYMVDVRKFRVNRYALRDNLAI
                     CFIYFSFIFLLNTFTNSRYYYGFLSHSTTAIFTFIFVTSIMYIPALLFNRDTFILEKK
                     KKSK"
BASE COUNT         1567 a          348 c          658 g         1192 t
ORIGIN      
        1 aatagacaac tttatccagc tgaaaatagt aaaaataatg ataaaaaaga ggggatgaag
       61 aataatatag attcaagtat attattagct gatgaccttt tgagcattta tagattatta
      121 aaaaagataa aaatacatga actttattca aatattaatt ttggaactgg aaatattggc
      181 ttaacatctt ctgtatatgt attgataaat actttgtatg gaaatttatt taatatgatt
      241 gatgcagaaa aaatgtattt aaatgtaaat ccagatttta caaaagatta tgttttagga
      301 aatataagga tacatataag gccaagaata aaagctttat ttaatattat aataatgatt
      361 aataaaatta tgaataaaaa taaaggaaat aaggaaggtg atagtaatga gagcaacagg
      421 ttcgatacag agtcttatgg aaacaactct tgaaacaatt aaaggttcta tagatgcaaa
      481 tactattata ggagacccta taaaaacaga tactacagtg gttgtaccta tatcaaaggt
      541 tacaattggc tttggtatag gaggaggaga atactcaaaa ggatatgatg ataaagatag
      601 ggaaattgaa ttaaaaaatg aaaaatcaga tacgaatttt gctggaggta gcgcaggggc
      661 tatatctgta caaccagtag catttgtagt agttgaaagt ggagagacta gaataatgag
      721 tttagacagt aatatcaatc tagtggataa tatattatct ataactccta gagtattgga
      781 gaaaattcaa aatatatcac aaagtaataa gaatcaagat aaaaacaata tgtaagaata
      841 aaatattttt aaatatgtat atagttttag gtaaagtagt ggggttagta taaatcccac
      901 tattttgttg tgtactttaa acatttatat ctaataagat tttagtttaa aacaatttaa
      961 atagataata aagttgatat atagtataat tataaaaaga aaagaagtat tatattatag
     1021 atatataaac gcatttgaga tataagaggg agttataaat cattagtttt gaggtgaatg
     1081 ttatgagcga taaagaatta tctattttgt tgttagaaag tatgaataat tttcataatt
     1141 taataaagat tattaataac gaaagatata aaaaagataa ggttcttaca gaaagacagt
     1201 tctttgcctt agtaaaaata agaaaacatg ataaaataga gcttaaaaat ctaagtagag
     1261 atttgcatgt atctacttct agtctgtgta tactacttaa taaattggtt gagcaagagt
     1321 atgtatatag ggaagaagat agtagagata gacgaaatac tttttatgga ataactaaaa
     1381 atggagaaaa gatattggat aatgaaatac taaagtttgt atctataata agtgataaaa
     1441 tggattgcct agatatagat aataaagata aattatttac ttctttggaa gagtctaaaa
     1501 atataataga acaattattt taaaaaggag aatttattat gaatataaag tattctaaag
     1561 aaatattaaa aagtttagaa aatgctataa cagtagtagc tctatttgag ggagaagatg
     1621 tttctacgac atttgataaa ataaatatga taagcagaaa ttttaattct aaagaaaata
     1681 gtatagatac tgtaacattg attgaaaata acaatttggt aaatgtttgt cttgttggtt
     1741 ttggtaagag agagttattg aatagagaaa aaattagaat cataggtggt aatttgtgta
     1801 aaaaattaaa aaatatattg aaaaatgaag catataagag tagtgatgtt aatgtactga
     1861 atcttaattt aaatagtgat gaaataggtt cttttgtaga agggatatta cttggaaatt
     1921 ataagtttga taagtataag acaaaaatta aggattttga aagaaaagaa gttgatacta
     1981 taactatatt tactgaaaaa gaagttgagt gtcaaataga aaaggctaaa atattagcta
     2041 attcaactat aatagctaga aatctagtta atgaaccatc aaacgtaata tatccaaaaa
     2101 cattagcagt agaaacagtt aaacttggtg cagagtttgg gtttaatgtt gatgtttatg
     2161 aagaagaaaa aataaaggca ttgggaatgg atgctttttt cgctgtttca agaggctcaa
     2221 ttaataaacc aagattcata gtaatgagat actttggaga taaagaaagt acagatatat
     2281 taggtttagt aggtaaagga ttgacttatg atactggtgg atattcttta aaatcaaatg
     2341 cttctatgct agatatgaag acagatatgg ctggtgctgc gagtgttata ggtgctatgt
     2401 gtgctatatc tcaaagtaaa ttaaagaaaa atgttatagc agtagtggct gcatgtgaga
     2461 atgctttatc aggaggttca tacaaacctg gagatataat tagttctatg gcaaagaaaa
     2521 ctattgaagt tttaaatacg gatgcagaag gtagacttac attagctgat gcaatatact
     2581 acattataaa caatgaaaaa gtaacaaagg tggttgatgt agctacatta actggtgctg
     2641 ctttaacatt gttaggtaat gttgcaactc ctatagtaac aaataatgat gatttttatt
     2701 gtgaattaga aaaagctgcc actttatcag gggaaagggt atggaagatg ccaatttatg
     2761 atgaatttaa agatatgata aaaggagaag aagcagacct taaaaacact ggtggtaaaa
     2821 atgctggatg tataacagca ggagctttta ttggtgaatt tgttgggaat actccttgga
     2881 tacatatgga tatagcgggg acaagtacat catcaaaatc aattggatac aaagcaaagg
     2941 gggctacagg agaacctgtt agaacattgt actatttggc agaaatctaa aaaacaatta
     3001 taaaatataa tttataaagc cacgtggata tttttgtaat ttaagattat ctatgtggct
     3061 ttatttttta tttaaataaa ttatttactc tttttctttt tttcaagtat aaaagtatct
     3121 ctattaaata aaagagcagg tatgtacata atactagtaa caaaaataaa cgtaaaaata
     3181 gcagttgtgc tatgacttaa aaaaccataa tagtacctag agttggtgaa tgtgtttagt
     3241 aaaaatatga atgaaaaata aataaaacat atagctaagt tatctctaag tgcatatctg
     3301 ttaactctaa attttctaac atcaaccata tatatatctg cataaattat caagcactta
     3361 gagaagataa agataaaata tattaagcta ggaaattcat agcccaagtt tttagaaaaa
     3421 atgagttctc caactgaaca aactaaactc caagaaaaaa atatattaaa aagatgatac
     3481 tgcttaaaaa ttagtatggc tatacacaga tatatacaaa tataattgat tccaattggt
     3541 aaagagttta gagtactata attacctgaa gaaataagta ataattgctc tagagccata
     3601 attattacaa gtaatgtaca aattatcttc tcaactgtat agctataagg taaaatattt
     3661 ttggttagtt ttggacaata gtataaaaat aatccaaata taaagagact tacaaattgt
     3721 tcactagaaa aaagaaaaat tttaaacata taacattctc tccta
//
LOCUS       DAEPIN010000158         3716 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.161, whole genome shotgun sequence.
ACCESSION   DAEPIN010000158 DAEPIN010000000
VERSION     DAEPIN010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3716
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.161"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            complement(230..517)
                     /locus_tag="KR250_003730"
     CDS             complement(230..517)
                     /locus_tag="KR250_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069609.1"
                     /translation="MRLNVTKQNTIEVENSRKEDVYFVIVDNVFFERQGDVFNSITNN
                     NLNNYDVKILPTDNVFLELKITSTKDLEPVFIVVEDRQYNHGSIMFKPVLV"
     gene            complement(537..812)
                     /locus_tag="KR250_003731"
     CDS             complement(537..812)
                     /locus_tag="KR250_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069610.1"
                     /translation="MSLFVGGWSKFRAVEEEDLKVFNEAVGMLEGVGYEPLIVSTQVV
                     AGTNFKFICNATSVTNPPQNYLAEIVVFKPLPCDEHNATITSINIIK"
     gene            complement(1449..1628)
                     /locus_tag="KR250_003732"
     CDS             complement(1449..1628)
                     /locus_tag="KR250_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069611.1"
                     /translation="MGKDRFEKVYSQGKINATEIWVDKETGVNYVFHYYGHAAGFTPL
                     LDREGKPVISPIVSK"
     gene            complement(1913..3388)
                     /locus_tag="KR250_003733"
     CDS             complement(1913..3388)
                     /locus_tag="KR250_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBH0069612.1"
                     /translation="MKRKPQYLIIFEHIIQQIYTGKITIGDRLDSIKSMSEEYMVSKN
                     TIKTVIKMLSEKGFIETKAGSRPVLIQSTSKQSIESDLLYEKILQISEIYKIFSLIFP
                     SIAMYNIKRFTSKDFEELNEIIDCMEKNSSNYLVFKEQRLKYINKLISKTNNFLIHHF
                     CEIIQHDVIISQLAYNFDNLDNLDNCCIDFFRNKYIEQVRKTYLYGLDGNLIEFKKLL
                     TYMYKACRESILKMVENRAPQSIDTSSFNSVTLEKCYLYDLIVSDVICQIFEGNIKIG
                     DCLPSITSIINRYNVSLPTVRSAYNELNELGIAKTINGKGTYITLFSEYDDDYINTEE
                     GAKRLSLLLDAMEFVTITLEDVVLLLGNKMDLIAVDKIETHLRYLQNNCEECKVYPDF
                     VLLCKIVDYTQIYVLQETFLHLKQYMIFGIYLERFFHEEYNEILKTRFDVCFEILRYL
                     KKGDVERFAESFSNIFKESLQYLKKVISSLNVDMRVKLDIL"
BASE COUNT         1301 a          580 c          378 g         1457 t
ORIGIN      
        1 gttcaactat actggtttaa ataggttatc tttatttgct taaatagttc aactatactg
       61 gtttaaatag gttatcttta tttgcttaaa taagttcaac tatactggtt taaataggtt
      121 atctttattt gcttaaatta gttcaactat actggtttaa atagttttcc tttagtttaa
      181 ataattcacc tatacctact tgaatagttc acttttattt gcttaaattc tataccaata
      241 ctggcttaaa cattatacta ccatgattgt attgtctatc ttcaactaca ataaaaacag
      301 gttctaaatc tttagtagat gttattttta attctaaaaa aacattatca gttggtaata
      361 tttttacatc ataattattt aaattattat tcgtgatact attaaataca tcaccttgac
      421 gttcaaaaaa aacattatca actattacaa aataaacatc ttcttttcta ctattttcaa
      481 cctctatagt attttgttta gttacattta gtctcatctt ccacctccta ttaatattac
      541 ttaattatat taatacttgt aattgttgca ttatgttcat cacaaggaag aggtttaaaa
      601 acaactatct ctgctaaata attctgtgga ggatttgtaa ctgaagtagc attacaaata
      661 aatttaaaat ttgttcctgc tacaacttga gtagaaacaa taagtggttc ataaccaacc
      721 ccttctaaca ttcctacagc ttcattaaat acttttaaat cttcttcttc gacagctctg
      781 aattttgacc atccaccaac aaaaagactc atataaaaac ctcctttata tatatattta
      841 tttatatata tagtagtacc aattttctca atattcaata ttttctgtgt aactggtcaa
      901 tgaaaatgca taactggata aaaatactta taaaataact taactttaca tataaatagc
      961 ttaatttaca tgtaataaga ttattctaca atatgaaata catcttattt tgaatatatt
     1021 ctaaatacaa taatggatat tttcaattat tatacttaga aaatatccat tacttaattt
     1081 gttttattta aaatcacata gagactttat tatttttttt attgcatgaa tagctatttc
     1141 aggtatcata aggtgatata ccccctcctt gaaaccacaa aaaacataac aaactttacg
     1201 aatcttgtct taaaagacca caggtacatt ccttatttgt ggattggagt agccataaca
     1261 agcaaatttt acaattttta tgatgacaaa aatcaataaa atcgcttagt atataacacg
     1321 aaatactact tgttgctatt aagaatagtt agataggaag tatgcattat accaataaag
     1381 gaatcaataa agaaagttaa aacatcccta caaattaaaa atctgttaca tttttctata
     1441 tttaaacgtt atttacttac aattggagaa ataacaggtt ttccttctcg gtcaagtaaa
     1501 ggcgtaaatc cggctgcatg gccataatag tggaatacat agttcacacc tgtttcttta
     1561 tccacccaaa tctccgttgc atttattttt ccttgtgaat atactttttc aaatctatct
     1621 tttcccattt tataatcctc catatttttg attggtctat gccttcttca cttaagtaga
     1681 aaaaatacat aacttatttt tttaattcta atatgagcac gtaaaaatgt caaacagctt
     1741 atttaatttc ataacttcat gttactgttc gtaaccttgt ctgtttactt acactgcatt
     1801 acagcaccat tttatccttt acattattgt attgcacaca gtaaaaatgc ttactttatt
     1861 ggatttttca actaaaaaaa ggattttttc tccacagaac atttactatt ttttataata
     1921 tatctaattt tactctcata tcaacattta atgaagatat aacctttttc aaatattgta
     1981 aagactcctt aaaaatatta gaaaaagatt ctgcaaatct ctcaacatct ccttttttta
     2041 aatacctaag tatctcaaaa catacatcaa aacgtgtttt caaaatttca ttatattctt
     2101 catgaaaaaa tctctctaaa tagattccaa atatcatata ttgttttaaa tgtaaaaatg
     2161 tttcctgtaa tacataaatt tgtgtataat ctactatctt acaaagtaaa acaaaatctg
     2221 gataaacctt acactcttca caattatttt gtaagtacct taaatgagtt tctattttat
     2281 ctacagctat caaatccatt ttatttccta gtaaaagtac tacatcctca agagtaatcg
     2341 taacaaactc catagcatcc aaaagcaaac tcaacctttt agctccttct tctgtattta
     2401 tataatcatc atcatattca gaaaaaagtg ttatatatgt tcccttgcca ttaattgttt
     2461 tagcaattcc caattcattt agctcattat aggcacttct aactgttgga agagatacat
     2521 tatatctatt aattatcgaa gtaattgatg gtagacaatc tcctatttta atattgcctt
     2581 caaaaatttg acaaattaca tctgaaacaa ttaagtcata taaataacat ttttccaacg
     2641 ttacagaatt aaaactactt gtatctatac tttgtggagc acgattttct accattttaa
     2701 gtatggattc tctacaagct ttatacatat atgttaatag ttttttaaat tcaattagat
     2761 tcccgtccaa tccatataag taagtttttc ttacctgctc tatgtattta ttacgaaaaa
     2821 agtctataca acaattatcc aagttatcca agttatcgaa attataggcc aattgtgaaa
     2881 taatgacatc atgctgaata atctcacaaa aatggtgaat caggaaatta tttgttttac
     2941 ttattagttt atttatatat tttagccttt gttctttaaa gactaaataa ttagaactat
     3001 ttttttccat acaatcgata atctcgttta attcttcaaa gtctttagat gtaaatcttt
     3061 taatattgta catagcaata gaaggaaata ttaaactaaa aatcttatat atttctgata
     3121 tttgtaaaat cttctcatat aataagtcag attctattga ttgtttactc gtactctgga
     3181 ttaaaactgg tctagaacca gcttttgtct ctataaagcc cttttctgag agcattttta
     3241 ttacagtttt tatagtattt tttgatacca tatattcttc tgacatgctt tttatagaat
     3301 caagcctatc tccaatagta attttaccag tataaatttg ttgaattata tgctcaaaaa
     3361 taattaaata ttgtggtttt ctcttcatat ttttcctctt cgataaatta gatttattgt
     3421 tttttgtaag gtatatttac ttttatttaa tagggcaaaa tagttgctta ttttttcctc
     3481 ataatctata attaatatag accatttata tatatggtct aagttttata atactgtaat
     3541 gcagtaaaat agcatattgt agctatacta cagcaaatta acataccatt gtagtatatt
     3601 aattggaata attttaagta cttcaaaatt ccagcaaaac atatatgtgt ttttgatatt
     3661 atgtatattc gttaatagaa tagctatact tgttttacat aataaattat atgata
//
LOCUS       DAEPIN010000159         3687 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.162, whole genome shotgun sequence.
ACCESSION   DAEPIN010000159 DAEPIN010000000
VERSION     DAEPIN010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3687
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.162"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            <1..238
                     /locus_tag="KR250_003734"
     CDS             <1..238
                     /locus_tag="KR250_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBH0069613.1"
                     /translation="RPNVKIEPKRVDSVNATNTKTYTVKAGDSLWSICQKQLGNGSLY
                     KKVYELNKTMMDKANKGKKVPKYTIYKGQVLRLV"
     gene            231..2123
                     /locus_tag="KR250_003735"
     CDS             231..2123
                     /locus_tag="KR250_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBH0069614.1"
                     /translation="MSDDLVLANDRDIRLVIAHWEDFYEPAVIDGITWEIERRGSPSK
                     LEFTIAMDDILEFCEGNSVRIYYKGIGIFYGYIFQKKRDKENHIKIVAYDQLRYFKNK
                     DTYVYSNKTASELVKMLAKDFNLKYNVIEDTKYKISRVEENKTLFDMVLTALDDTLRE
                     KKEMYVLYDDFGRITLKNVASMKLDTVMNNDVIEDFDYNSSIDSDTYTKIKLVRDNEE
                     TGKRDVYIAQDSTHMRSWGILQMFDTVDKNMSEAEIKQKCDILLKLYNKKTKSLSLKN
                     VLGDIRVRAGCLVPVFLNLGDIKLQNYMLVEKVKHTFENNSHFMDLTLVDGDEFASYS
                     SSSYSSGNTNNKDEKKNGPAQSITKKNTGKKVPAIFTAYYPGNNAMEGGKTDCNGKPL
                     DVKSRTVAGPMNREGVKKTWYTDDFLKKHPVFEYGDKVKIILPGTAYDNKVYTVKDNG
                     GRIYVETNGTYHIDVLLANASECKKFGRKNGYIIIGGDEEQTYQVEGNNQSSTNNNSK
                     EDKLISIAKSKLGCNYVYGAEGPNNFDCSGFTQWCYKQIGIKIPRTASAQSKAGKAVD
                     LKDRSKWKAGDLLCRIGGGSSNHVVMYIGNNQIIHSPQTGDVVKIESVNSYRKGKAYT
                     HVRRFI"
     gene            2137..2397
                     /locus_tag="KR250_003736"
     CDS             2137..2397
                     /locus_tag="KR250_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2577 domain-containing protein"
                     /protein_id="HBH0069615.1"
                     /translation="MSQDLLQIIKKAAMDAVETSNPMRVVFGTIESISPLRVKIEQKL
                     SIGEIFLIQTDTFKRYTDKKIGDKLVLIRMQGGQQYLILDRM"
     gene            2402..2821
                     /locus_tag="KR250_003737"
     CDS             2402..2821
                     /locus_tag="KR250_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HBH0069616.1"
                     /translation="MLPSDNLDYDIEDVSIINFDVRQEPSKTFKLHIEKSKIDGICDD
                     VEALKQTIFLILNTERYQHLIYSWNYGVELNDLIGEPISFVIPELERRIKEALIQDDR
                     VENVDNFEFENIKGKVQCKFSVHTKYGNIKAEKVVSV"
     gene            2834..>3687
                     /locus_tag="KR250_003738"
     CDS             2834..>3687
                     /locus_tag="KR250_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HBH0069617.1"
                     /translation="MTFENIIKRMLDSVPNTLDKREGSIIYNALAPVAVELTETYIAM
                     DELLDQTFVDTASYYYLEKRCKERGITPLPATNTIAKGVFNIDIPLDSRFNLGEYNYI
                     AIERISEKTYKMKCETAGPIFELGKLIPIEYIDGLETAELTEILINGEDEESEDSLRQ
                     RYYDSLNSQSFGGNIQNYKDEVNKIQDVGGVKVYPVWDGGGTVKLVIINSNFKVPSED
                     LVNLVQEEIDPIGHQGQGLGLAPIGHKVTVTGVVSTTINISAEITYKNGYTWENIKSI
                     AEEAIDDY"
BASE COUNT         1493 a          409 c          735 g         1050 t
ORIGIN      
        1 tagacctaat gtaaagatag agccaaaacg agttgattca gtcaatgcca caaacactaa
       61 aacatataca gtaaaagcag gggatagcct ttggtcaatt tgtcagaagc aacttggtaa
      121 tggttcgtta tacaagaaag tatatgagtt aaataaaaca atgatggata aagctaacaa
      181 gggtaaaaaa gtacctaaat ataccatcta taaagggcag gtgttgaggc ttgtctgatg
      241 atttagttct ggcgaatgat agagatataa ggctagttat tgcacattgg gaagatttct
      301 acgaacctgc tgtcattgat ggtatcacat gggagataga aagaagaggt tcaccttcaa
      361 agttagagtt tactatagca atggatgata tattagagtt ttgcgaaggt aattctgtaa
      421 ggatatatta taagggaatt ggcatatttt atggatatat atttcagaag aaaagagata
      481 aagaaaatca cattaaaata gttgcttatg accagttgag atattttaaa aataaagata
      541 cttatgtata tagtaataaa acagcaagtg aacttgtaaa gatgttggct aaagatttta
      601 atttaaaata caatgtcata gaagatacaa aatataaaat atcgagagtt gaagaaaata
      661 aaacactctt tgatatggtc ttaactgcac tagatgatac tctaagagag aaaaaagaaa
      721 tgtatgtgtt atatgatgat tttggaagaa taacattaaa gaatgttgca tcaatgaaat
      781 tagatactgt tatgaacaat gatgtaatag aagactttga ctataattca tcaatagata
      841 gtgatactta cacaaagatt aaacttgtga gagataatga agaaacagga aaaagagatg
      901 tgtatattgc tcaagactca acgcatatga ggagttgggg aatacttcaa atgtttgata
      961 cagtagacaa aaacatgagt gaagcagaga taaaacaaaa gtgtgatata cttctaaaac
     1021 tatataataa gaaaactaag tcattaagtt taaaaaatgt acttggagat attagagtga
     1081 gagcaggttg tttagtacct gtttttttaa atctaggaga tattaaactt caaaattata
     1141 tgctagttga gaaagtaaaa catacttttg aaaataacag tcattttatg gatttgactc
     1201 ttgttgatgg agatgaattt gcttcttatt cttcaagctc atattcaagt ggaaatacta
     1261 acaataagga tgaaaagaaa aatggtcctg cacaaagtat tacgaaaaaa aatacaggta
     1321 aaaaagttcc tgctatattt actgcatatt atccagggaa caatgcaatg gaaggcggaa
     1381 aaacagattg caatggaaag ccacttgatg taaaatcaag aactgttgct ggtccaatga
     1441 atcgagaagg agttaagaaa acttggtata ctgatgattt tctaaagaaa catccagttt
     1501 ttgaatatgg agataaagta aaaattatac ttcctggtac tgcctatgac aacaaagtat
     1561 atacagttaa agataatgga ggaagaatat atgttgaaac aaacggaaca tatcatatag
     1621 atgtactatt agctaatgct agtgaatgta aaaaatttgg tagaaagaat ggctatataa
     1681 ttataggtgg agatgaagaa caaacatatc aagttgaagg taataaccaa agtagtacaa
     1741 ataataactc taaagaagat aaattaatta gtatagcaaa aagtaaactg ggttgtaatt
     1801 atgtgtatgg agcagaaggt cctaataatt ttgattgcag tgggtttact caatggtgtt
     1861 ataaacaaat aggtataaaa attcctcgta ctgcttctgc acaaagtaaa gcaggaaaag
     1921 cagtagattt aaaagataga agcaagtgga aagcaggaga cttattatgt agaattggag
     1981 gaggaagtag taatcatgtt gtaatgtata ttggaaacaa tcaaataatt cattcaccac
     2041 aaacaggaga tgtggtaaaa atagagtctg ttaattctta tagaaaagga aaagcataca
     2101 cacatgtgag aagatttata taagtgaggt ggcaatatga gccaagattt attacagata
     2161 ataaaaaaag ctgcaatgga tgcagtagaa acaagcaacc caatgagggt tgtatttgga
     2221 acaatagaaa gtattagtcc tctaagagtt aagatagaac aaaaactatc tattggtgaa
     2281 atttttctaa tacaaacaga tacatttaaa agatatacag ataaaaaaat aggagataaa
     2341 ttagtcttaa ttcgtatgca aggagggcaa caatacttga ttttagatag gatgtgataa
     2401 agtgttacca agtgataatt tagattatga tattgaagat gtatcaataa ttaattttga
     2461 tgtaagacaa gagccaagta agacatttaa acttcatata gaaaagtcta agattgatgg
     2521 tatttgtgat gatgtagaag cattaaaaca gaccatcttt ttgattttaa acacagagag
     2581 ataccaacat ctaatatata gttggaatta tggagtcgag ttgaacgacc ttattggaga
     2641 gcctatatcc tttgtaatac cagagttaga gagaagaatc aaagaagcac taattcaaga
     2701 tgatagggtt gaaaatgtag ataattttga gtttgaaaat ataaagggta aagtacaatg
     2761 taaattttca gttcatacaa aatatggaaa tataaaagca gagaaggtgg tgagtgtata
     2821 attgtttgag ttaatgacat ttgaaaatat aattaaaaga atgttagata gtgtacctaa
     2881 cacacttgat aagagagaag gttctataat atacaatgcc ttggcaccag ttgcagtgga
     2941 gcttacagaa acatatattg ctatggatga attactagac caaacattcg tagatactgc
     3001 tagttattac tatttagaga agagatgtaa agagcgagga attacacctt taccagccac
     3061 taatacaatt gctaaaggag tttttaacat agatattcca cttgattcta ggtttaatct
     3121 aggagaatac aattatattg caattgagag aatatctgaa aaaacatata aaatgaaatg
     3181 tgaaactgct ggacctatat ttgagttagg aaaactaata cctattgaat atatagatgg
     3241 tcttgaaact gctgaactaa ctgaaatctt gataaatgga gaggatgaag agtcagaaga
     3301 tagtttaaga caaagatatt atgatagcct aaattcacag agctttggtg ggaatataca
     3361 aaactataaa gatgaagtta acaaaataca agatgttgga ggagttaagg tttatcctgt
     3421 gtgggacggt ggaggaactg ttaagttagt aataattaac tctaatttca aagtaccatc
     3481 agaggattta gttaatttag tgcaagaaga aattgaccca attggacatc aaggacaagg
     3541 cttaggatta gcaccaatag ggcataaagt tactgttaca ggtgttgtaa gtacaactat
     3601 aaatatatca gcagagataa catacaaaaa tggctacact tgggagaata taaaatcaat
     3661 tgcagaagaa gcaatagacg actattt
//
LOCUS       DAEPIN010000160         3581 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.163, whole genome shotgun sequence.
ACCESSION   DAEPIN010000160 DAEPIN010000000
VERSION     DAEPIN010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3581
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.163"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            <1..244
                     /locus_tag="KR250_003739"
     CDS             <1..244
                     /locus_tag="KR250_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBH0069618.1"
                     /translation="RPNVKIEPKRVDSVNATNTKTKTYTVKAGDSLWSICQKQLGNGS
                     LYKKVYELNKSMMDKANKGKNLSKYTIYKGQVLKLG"
     gene            237..2207
                     /locus_tag="KR250_003740"
     CDS             237..2207
                     /locus_tag="KR250_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBH0069619.1"
                     /translation="MVDELVLANDRDVRLVIAHWEDFYEPAVIDGITWEIERRGTPSK
                     LEFTIVMDDILEFCEGNSVRLYYKGIGIFYGYIFQKKRDKENHIKIVAYDQLRYFKNK
                     DTYVYSNKTASELVKMLAKDFNLKYNVIEDTKYKISRIEENKTLFDMILTALDDTLRE
                     KKEMYVLYDDFGRITLKNVASMKLDTVMNNDVIEDFDYNSSIDSDTYTKIKLVRDNEE
                     SGKRDVYIAQDSAHMRSWGILQMFDTVDKNMSEAEIKQKCDILLKLYNKKTKSLSLKN
                     VLGDIRVRAGCLVPVFLSLGDIDLQNYMLVEKVKHTFENNSHFMDLTLVDGDEFASYS
                     SSSYSSGNTNNKNEKQNGPAQSTTSKEDTDMANKINKLLKGKLSNTGNIFVKYSNAYK
                     VNPALMAAISMHESARGTSNIANTKNNFFGMKKNGDYMSFSSVDEGIKRGISNLSRNY
                     IHIGRKTLESIRNKYSSSSDKEWVKCVGAFYKQITGSTYNSNSAGTGVGSNEEAEKNL
                     KDLTYQVQNNNSNTSTNNNNKVSKVIQEAKNQLGKPYKWGGNGPKSFDCSGLMVWAFK
                     RGAGINLKRVSADQSKDSRGKLLCNINDVKAGDLVFFAYNKGKGNVHHVGLYIGNDQY
                     IHAPQTGDVVKISSLSGRQKKKHDFARARRFF"
     gene            2221..2481
                     /locus_tag="KR250_003741"
     CDS             2221..2481
                     /locus_tag="KR250_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2577 domain-containing protein"
                     /protein_id="HBH0069620.1"
                     /translation="MSQELLQIIKKAAMDAVETSNPMQIAFGTIESVNPLIVKIEQKA
                     SFEEFFLIQTETFKRYTDKKIGDKLVLIRMQGGQQYLILDRM"
     gene            2486..2905
                     /locus_tag="KR250_003742"
     CDS             2486..2905
                     /locus_tag="KR250_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HBH0069621.1"
                     /translation="MLPTDNIDYDIEDVSIINFDVRQEPSKTFKLNIEKNRVDGICDD
                     VEALKQTIFLILNTERYEHLIYSRNYGVELNDLIGEPISYVIPELERRITEALIQDDR
                     IENIDNFEFQNIKGKVQCRFSVHTKYGNIKAEKVVSV"
     gene            2918..>3581
                     /locus_tag="KR250_003743"
     CDS             2918..>3581
                     /locus_tag="KR250_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HBH0069622.1"
                     /translation="MTFENIIKRMLDSVPDTFDKREGSIIYNALAPVAIELTETYIAM
                     DELLDQTFVDTASYYYLEKRCKERGITPLEATNTIAKGVFNIDIPIDSRFNLGEYNYV
                     AIERISEGIYKMKCETAGPIFELGQLIPIEYIDKLETAELTEILINGEDEESEDSLRQ
                     RYYDSLNSQSFGGNMQNYKDEVNKIQDVGGVKVYPVWDGGGTVKLVIINSNFKVPSED
                     LVN"
BASE COUNT         1464 a          382 c          690 g         1045 t
ORIGIN      
        1 tagacctaat gtaaagatag agccaaaacg agttgattca gtcaatgcca caaacactaa
       61 aactaaaaca tatacagtaa aagcagggga tagcctttgg tcaatttgtc agaaacaact
      121 tggtaatggt tcattatata agaaagtata cgaactaaat aaatctatga tggataaggc
      181 aaataagggc aaaaacttaa gtaaatacac tatttataaa gggcaggtgt taaaacttgg
      241 ttgatgaatt agtgttagca aatgatagag atgtaaggtt agtaatagct cattgggaag
      301 atttctacga acctgctgtc attgatggta tcacatggga gatagaaaga agaggaacac
      361 cttctaagtt agaatttaca atagttatgg atgatatatt agagttttgc gaaggaaatt
      421 ctgtaaggct gtattataaa ggaataggta tcttctatgg atatatattt cagaagaaaa
      481 gagataaaga aaatcacatt aaaattgttg cttacgacca gctaagatat tttaagaata
      541 aagatactta tgtatatagt aataaaactg caagtgaact tgtaaaaatg ttggctaaag
      601 attttaattt aaaatacaat gtcatagaag atactaagta taaaatatct aggatagaag
      661 aaaataaaac actctttgac atgatactaa cagcactaga tgatactcta agagagaaaa
      721 aagaaatgta tgttttatat gatgattttg gaagaataac attaaagaat gttgcatcaa
      781 tgaaattaga tactgttatg aataatgatg tcatagagga ctttgactac aattcttcta
      841 ttgatagtga tacttacaca aagattaaac ttgtaagaga caacgaggag tcaggaaaaa
      901 gggatgtata tattgctcaa gactcagctc acatgagaag ttggggaata cttcaaatgt
      961 ttgatacagt agataaaaac atgagtgaag cagaaataaa gcaaaagtgt gatatacttc
     1021 taaaactata taataagaaa actaagtcat taagtttaaa aaatgtgtta ggagatatta
     1081 gagtaagagc aggttgttta gtacctgttt ttttgtcgtt aggagatatt gatttacaaa
     1141 attatatgtt agttgagaaa gtaaaacata catttgaaaa taacagtcat tttatggacc
     1201 taactttggt tgatggagac gaatttgctt cttattcttc atcaagttat agtagtggaa
     1261 atactaataa taaaaatgag aaacaaaatg gtcctgcaca aagtactaca agtaaagaag
     1321 atactgatat ggctaataag attaataaac tacttaaagg taaattatca aatacaggaa
     1381 atatatttgt taaatattca aatgcttata aagttaatcc agcactcatg gctgctatat
     1441 ctatgcacga atcagctaga gggacttcaa atattgcaaa tactaaaaat aatttctttg
     1501 gaatgaaaaa aaatggagat tacatgagtt tttctagtgt agacgaagga ataaaaagag
     1561 gtataagtaa tttatcaaga aactatatcc atataggacg aaaaacttta gaaagcatca
     1621 gaaataaata ttcttctagt tcagacaaag aatgggtaaa atgtgtaggt gcattttata
     1681 agcaaataac aggaagtact tataattcta atagtgcagg cacaggagtt ggaagtaatg
     1741 aagaagcaga aaagaattta aaagatttaa cttatcaagt tcaaaacaat aattctaata
     1801 catcaacaaa caataataat aaagtaagta aagttattca agaagcaaaa aatcaacttg
     1861 gcaagcctta caaatggggt ggtaatggtc caaagagttt tgactgtagt ggtcttatgg
     1921 tgtgggcatt taaaagaggt gcaggaataa atctcaaaag agtttcagca gaccaatcaa
     1981 aagatagtag aggaaaacta ttatgtaaca taaatgatgt aaaagctggt gatttagtat
     2041 tctttgcata caacaaagga aaaggaaatg tacatcatgt tggactatat ataggaaatg
     2101 accaatatat tcatgctcca caaactggtg acgtagtaaa aataagtagt ttaagtggta
     2161 gacaaaagaa aaagcatgat tttgcaagag ctagaagatt cttttaagtg aggtgataaa
     2221 gtgtcacaag aattattgca aataattaag aaggctgcaa tggatgcagt agaaacaagc
     2281 aatccaatgc aaattgcatt tggaactata gaaagtgtta atcctttgat agttaagata
     2341 gaacaaaaag catcttttga agaatttttt ctaatacaaa cagagacttt taaaagatat
     2401 acagataaaa aaatagggga taaattagtc ttaattagga tgcaaggagg acagcaatat
     2461 ttgattttag ataggatgtg atgaaatgtt accaacagat aacattgact atgatataga
     2521 agatgtatcg ataattaatt ttgatgttag acaagaacca agtaagacgt ttaaattgaa
     2581 tatagagaaa aatagagtag atggtatttg tgatgatgta gaagcattaa aacaaaccat
     2641 ttttttaatt ttaaatactg aaaggtatga gcaccttatt tattctagaa attatggtgt
     2701 tgaattaaat gatttaattg gagaacctat ttcatatgta atacccgaac ttgaaagaag
     2761 gataacagaa gcactaattc aagatgatag gattgaaaat atagataatt ttgagtttca
     2821 aaatataaag ggtaaagtac aatgtagatt ttcagttcat acaaaatatg gaaatataaa
     2881 agcagagaag gtggtgagtg tataattgtt tgagttaatg acatttgaaa atataattaa
     2941 aagaatgtta gatagtgtac cagatacttt tgataaaagg gaaggttcta taatatataa
     3001 tgctcttgct cctgttgcta tagaacttac agaaacatac attgctatgg atgaattact
     3061 agaccaaaca ttcgtagata ctgctagtta ttattattta gagaagagat gtaaagagag
     3121 aggtattaca ccacttgaag ccactaatac aattgcaaaa ggagttttta acatagatat
     3181 tcctattgat tctaggttta atctaggaga atataactat gtagcaattg agagaatatc
     3241 tgaaggtata tataagatga aatgtgagac tgcgggacct atttttgagt tgggacaact
     3301 aatacctatc gaatatatag acaaattaga aacagcagaa ctgacagaaa tactgataaa
     3361 tggagaagat gaagagagtg aggatagttt aagacaaaga tattatgata gcctaaattc
     3421 acagagcttt ggtggaaata tgcaaaatta taaagatgaa gttaacaaaa tacaagatgt
     3481 tggaggagtt aaggtttatc ctgtgtggga cggtggagga actgttaagt tagtaataat
     3541 taactctaat ttcaaagtac catcagagga tttagttaat t
//
LOCUS       DAEPIN010000161         3568 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.164, whole genome shotgun sequence.
ACCESSION   DAEPIN010000161 DAEPIN010000000
VERSION     DAEPIN010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3568
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.164"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     repeat_region   169..592
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=169..197
                     /rpt_unit_seq="gttttagattaactatatggaatgtaaat"
     gene            756..929
                     /locus_tag="KR250_003744"
     CDS             756..929
                     /locus_tag="KR250_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HBH0069623.1"
                     /translation="MSSKMGRPKVDNPKNIDVKVRFDENTHKKLLDYCEKENLTRTEA
                     IRKGVDLLLEEDK"
     gene            1053..1880
                     /locus_tag="KR250_003745"
     CDS             1053..1880
                     /locus_tag="KR250_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin Bro"
                     /protein_id="HBH0069624.1"
                     /translation="MNNELMNFENNELGIKIRTIKYEDGSIGINAEDTAVGFGWCQTQ
                     NKNGKQYVSIRWETINKYCKEFGFLNLLGKDEYIPESLFYLLGMKAKNEIAVKFQTWL
                     AVDVLPSIRQTGAYITNNANPEKLREKASEIEKLQLAYNSTSMLKELLDGAGFDNKSK
                     LLTAKTLYKKAGIDLPIEIEEEESFFDTKQIASKLKIYSKSNKPAQLAVCEIIKKIDL
                     EENEVKGVWETNGSWTGTVNKYTKSVIDKVRNWIEENNRPAKIAGEKKNYHVVYKIE"
     gene            1943..2119
                     /locus_tag="KR250_003746"
     CDS             1943..2119
                     /locus_tag="KR250_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069625.1"
                     /translation="MCENLLNEYNLKTDEDVEYFVKFATLLYKLKQDNEEKFQEYAEI
                     LRGILREQQERENK"
     gene            2307..2519
                     /locus_tag="KR250_003747"
     CDS             2307..2519
                     /locus_tag="KR250_003747"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069626.1"
                     /translation="MLNSFLDFILTKWYVNVNSFNLLIEDAFRFILTKWYVNVSTFDM
                     CCGVMFMFYINYVDSKLNRKKKHLLK"
     gene            complement(2532..2735)
                     /locus_tag="KR250_003748"
     CDS             complement(2532..2735)
                     /locus_tag="KR250_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008982964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0069627.1"
                     /translation="MYFNIDKLLESKGRTRYWLAKEVGIAYPNMMKLANNETSSIKLD
                     LFEKLCLVLECTPNELVTLSSKE"
     gene            2979..3365
                     /locus_tag="KR250_003749"
                     /pseudo
     CDS             2979..3365
                     /locus_tag="KR250_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013726857.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
BASE COUNT         1406 a          361 c          626 g         1175 t
ORIGIN      
        1 tagagatttt gcagtgagca atatttgcga taaattgaag tttaacaatt ggaatacaag
       61 gtattgaggg tgtgtgataa atgttatcaa ttgcactact catggttcac tgcaaatttg
      121 agagagatgc gtatgtgtag gtattggaaa tgccaagttt attttggggt tttagattaa
      181 ctatatggaa tgtaaattaa cctttttatt gttgttgtta ttcttattgt tattcgtttt
      241 agattaacta tatggaatgt aaatgttgta agaagtatca ttctattttt taatctttct
      301 gttttagatt aactatatgg aatgtaaata gtacatataa tgagtcttta acatcagtta
      361 tgaaaggttt tagattaact atatggaatg taaatgattg tactttagcg tctgcactag
      421 ctttgtctat cgttttagat taactatatg gaatgtaaat tattttacag atgaacaatt
      481 acagttactt cttgaatgtt ttatattaac taagtggtat gtaaataatg gaagcaagat
      541 aatttttaaa ggtatggata attgttttat attaactatg tggattcaaa attaaataga
      601 aaaaagaaag cacttactta aatagtaggt gcttttgttt tgctcaaaat tggtcggttg
      661 agtaaaataa ttagaaaaaa ttgggataag ttattgactt tttggaacac aaataatata
      721 attatattat ggaacacaaa aagtgaggtg aaacaatgag ttctaaaatg ggaagaccaa
      781 aagttgataa tcctaaaaat attgatgtca aagtaagatt tgatgaaaat actcataaaa
      841 aattattaga ttattgtgag aaagaaaatc ttactagaac agaagcaata agaaaaggag
      901 tagacttact tttggaggaa gataagtaaa aaagtagccc aacgccgacc aaagcatact
      961 ggactactta acctagagtt atctctatat gaaatattct atcatgtaaa gataactctt
     1021 tcaagataat aaatcgaaag ggtgattttt gtatgaataa tgaactgatg aattttgaaa
     1081 ataatgaatt aggaataaaa ataagaacta ttaaatatga agatggaagt ataggaatta
     1141 atgcagagga tacagctgta gggtttggat ggtgtcagac acagaataaa aatggtaagc
     1201 aatatgtatc aattagatgg gaaactatta ataaatactg taaagaattt ggtttcctca
     1261 acttgttggg gaaagatgag tatatacctg aatcattatt ttatctttta ggtatgaaag
     1321 ctaaaaatga aatagcagta aaatttcaaa catggttagc agtagatgta ctaccatcaa
     1381 taagacaaac tggtgcatac ataactaaca atgctaatcc cgaaaaacta agagaaaaag
     1441 caagtgagat tgaaaagtta caattagcct ataacagcac atctatgtta aaagaattgc
     1501 tagatggtgc aggatttgac aataaatcca aactattaac agctaaaaca ttatataaaa
     1561 aggcaggcat tgatttacct atagaaattg aagaagagga atctttcttt gacacaaaac
     1621 aaatagcatc taaactgaaa atatattcta agagtaataa accagcacag ttggctgttt
     1681 gtgaaattat taaaaagatt gatttagaag aaaacgaagt caaaggcgtt tgggaaacta
     1741 atggttcttg gactggtact gtaaataaat atacaaagag tgtaatagat aaggttagaa
     1801 attggataga ggaaaataat agacctgcta agattgcagg tgaaaagaag aattatcatg
     1861 tggtttataa gattgagtaa atttatcagt tgtattaaat aatttagttt agtttatttt
     1921 agttttgagg gggattaata caatgtgtga gaatttactt aatgaatata atttaaaaac
     1981 tgatgaagat gtagaatact ttgtaaagtt tgctacatta ttatataaat taaaacagga
     2041 caatgaagaa aaatttcaag agtatgcaga gatattgaga ggtattctta gggaacaaca
     2101 agagagagaa aataagtaaa ataatataga taaagcactt gaatattaca tagtttcaag
     2161 tgctttgttt ggtataaaat ggtataatag agataagagt tatattaact atgtggtatg
     2221 taaagaatgt ataaggcatc ttctcaaatt ctgcgttggt cattttatat taactatgtg
     2281 gtatgtaaag tgcgttaact gcaataatgc tgaactcatt tcttgatttt atattaacta
     2341 agtggtatgt aaatgttaat tccttcaacc ttctaataga agatgctttt aggtttatat
     2401 taactaagtg gtatgtaaat gtttcaacat ttgacatgtg ctgtggagta atgtttatgt
     2461 tttatattaa ctatgtggat tcaaaattaa atagaaaaaa gaagcactta cttaaatagt
     2521 aggtgctttt gttattcttt gctagataaa gtaacaagtt cattaggtgt acattcaaga
     2581 acaagacaaa gcttttcgaa taaatctaat tttatagaac ttgtttcatt gttagctaat
     2641 ttcatcatgt taggataagc tatacctact tctttagcta accaatatct agttcgacct
     2701 ttggattcaa gtaatttatc aatgttaaaa tacatttttt cacctcttaa gtatattata
     2761 caacaattat ataatgtttg caatatataa tatgcaaaca atataattat ataatgttga
     2821 cattatataa tgtgtgcgat ataatatact taaggaataa aaaataagag ccactcgccc
     2881 ctaccaagtt tgagtaactc ttattgacat atactacata tacattaatt atagtatatg
     2941 tcattcctta aaaaaaatca attaaggagt gtgtttttat gaacaattta gtactaatta
     3001 acaatcaaga agtgcaagtt aaggaattta ataatcaaag agtagttaca tttaaagaaa
     3061 ttgacagagt acatgaaaga gtagaaggaa cagcaggtag aaattttaga gaaaataaga
     3121 agcactttat aaaaaatgag gactattttt atttagaagg aaaagaatta tcaacaatta
     3181 aacagacaac gaatttcgtt ggcagtaatg caagagaatt aatcttatta actgaaacag
     3241 gttatctaat gttagtaaaa tcttttacag atgatttagc ttggaaagta caaagacaat
     3301 tggtaaatag ttattttaga gttaaagaag aaaagaaaga aatgaaagca ttagaaaaat
     3361 tagaaacagt taatgagtca attaggttaa taactccaat ttttgatgat ttaaacattg
     3421 ataaaagtat gaaacttctt gtaactaaga ctttttttga aagagcaggt attgaattgc
     3481 ctcttgaagt tgaagaaaaa gaacattttt atgatacaaa gcagattgca aaaaaattag
     3541 gactttacac aatgtctaat aaaccagc
//
LOCUS       DAEPIN010000162         3536 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.165, whole genome shotgun sequence.
ACCESSION   DAEPIN010000162 DAEPIN010000000
VERSION     DAEPIN010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3536
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.165"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            395..1108
                     /locus_tag="KR250_003750"
     CDS             395..1108
                     /locus_tag="KR250_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0069628.1"
                     /translation="MDTCFNKKILLVDDEKDIVDLIEEVLINDGFKNIIKAYNGLDAI
                     SLCKIACPDVVILDIMLPDIDGIEVCKKIREFSYCSILFLSSKNDDIDKILGLSSGGD
                     DYITKPFSPREIAFRVKAQLRRQQYQSIVPSDSEVIKIGDITIDIEGNRVYKDRNEIE
                     LTGREYHLLSYMAQNVNKIIGKERLYEQVWGVYSSICDNTIMVHIRHIREKIEDNPSN
                     PKILITVKGLGYKLVNRID"
     gene            complement(1380..2918)
                     /locus_tag="KR250_003751"
     CDS             complement(1380..2918)
                     /locus_tag="KR250_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBH0069629.1"
                     /translation="MSLLKVTNLSQCFMDKSLYEKANFDLFKGEHIGVVGQNGTGKST
                     LIKILLGEVVPDSGEIKWQPNINIGHLDQYAEINRDTTISLYLHTAFEELYKIEKEMN
                     LLYQKSAISENEQYLIKASDYQEQLIASNFYSIDNEINKIANGLGLDSIGMNRVVREL
                     SGGQRAKVILAKLLLSNHDVLLLDEPTNFLDKEHVEWLSNYLNTFNGAFIVVSHDFDF
                     LEKISTGILDIEFGMIKKYHGKYSEFLKQKSHLREDYIRRYQAQQKKIEKEETFIRKN
                     KAGVNSKIARGRQKQLDKIERIAPPSFTGKPNIQFSEIEISAQNALTITNLEVGYYYS
                     LLPKLNFSVDGGQKIVITGFNGIGKSTLLKTLVKDIPRISGDFQFSEQVKIGYYEQDL
                     KWENPDKTPLQIVADKYPKLNTKEIRRHLARCGVKEEHVSRSVSTLSGGEQSKVKLCC
                     MMLSPCNFLILDEPTNHFDAETKDALQNALKQFRGSIILVSHEEKFYKGWIDKVFNIE
                     KQLV"
BASE COUNT         1241 a          464 c          497 g         1334 t
ORIGIN      
        1 tagtggtaga tatatactat tgaaaattaa agttataatt gatataaaga attaataaaa
       61 ataacatata aaagtttaaa agggagttga acttaaagtt tgattagaaa aagtcaattt
      121 taagacaact ccttttattt tactattgta ttaatctgta tctgtaagtg tattagtcta
      181 atattataaa ctttggatga aattaatttt ctataggttt taatgtatca tacactttat
      241 tttaaagaaa cttatcttaa ttttcatagt gattgaattt gtctattaga gatgttacta
      301 ttagtaatgt aaaagcaaga gtagtgaata taaaaaacaa gatagtgata ctgcaagatt
      361 agaatataat aaatttttat ggagagattt caatatggat acatgtttta ataaaaaaat
      421 attacttgtt gatgatgaaa aggatatagt agatttaatt gaagaagtac tcataaatga
      481 tggttttaaa aacattatta aagcttataa tggtctagat gctatttcgc tgtgtaagat
      541 agcatgtcca gatgttgtca ttcttgatat aatgttacca gatatagatg gtatagaagt
      601 gtgtaaaaaa attcgagagt tttcatactg ttctattctt tttttatcat caaaaaatga
      661 tgatattgac aaaatactag gtcttagtag tgggggagat gactatataa caaagccatt
      721 cagtccaaga gaaattgctt ttcgagttaa agcgcaatta cgtagacaac agtatcaaag
      781 cattgtacca tcagatagtg aagttattaa aataggagat attactattg atatagaagg
      841 aaaccgtgta tataaagata gaaatgagat tgagttaact ggaagggagt atcatctttt
      901 aagctacatg gctcaaaatg taaataaaat tataggaaaa gaaagactct atgaacaagt
      961 atggggagta tatagtagta tttgtgacaa tacaattatg gtgcatatac gacatataag
     1021 agaaaaaatt gaagataacc cctcaaatcc taaaatactt ataacagtta aaggactagg
     1081 atataaactt gtgaatagaa tcgattagaa tagattttaa tattaatgaa gatatctcaa
     1141 gttgaatttt tagttaatga ggcattcttt tatgggtgaa atcaaatgta ttttcgttat
     1201 ctcaacaatg aagaagaggt ctatctctat tttgagatag cctcattaag tttatatcct
     1261 ggtaatgtac tttatgttct aataaaattt ttttagttta tttgcagtga aacattaact
     1321 agaacattaa aatttgcatt gaaataccaa ttaaataata ttgtatattg tgtataaact
     1381 tatactaatt gtttttctat attaaatact ttatcaatcc aacctttata aaatttttct
     1441 tcatgggaaa ctaggattat acttcctcta aactgtttca aagcattttg taaagcatcc
     1501 tttgtctctg catcaaagtg attggttggt tcatccagaa taagaaaatt acaaggagat
     1561 aacatcatac aacataattt aaccttagat tgttcaccac cacttaaagt agatacactc
     1621 cttgaaacat gctcttcctt cacaccacat ctggctagat gacgtctaat ctcttttgta
     1681 tttaattttg gatatttatc tgctactatt tgaagtgggg ttttatcagg attttcccat
     1741 tttaaatctt gctcataata accaatttta acttgttctg aaaactggaa atctccagat
     1801 atacgaggaa tatctttgac taatgtcttt aacaaggtag atttaccgat tccattaaaa
     1861 ccagttataa ctattttttg acctccatca acagagaaat ttaattttgg aagtaaagaa
     1921 tagtagtatc caacttcaag atttgttata gtaagagcat tttgagccga tatttcaatt
     1981 tctgaaaatt gaatatttgg ttttcctgta aaactaggag gagctattct ttctatttta
     2041 tccaattgtt tttgtcttcc tcttgcaatt tttgaattta ctccagcttt atttttacgt
     2101 ataaatgttt cttctttttc tattttcttt tgttgtgctt gataccttct aatataatct
     2161 tctcttaaat gtgatttttg tttaaggaac tctgaatatt ttccatgata tttcttaatc
     2221 atgccaaatt ctatatcaag aatacctgta gaaatttttt ctaaaaagtc aaaatcgtgg
     2281 gaaactacaa taaatgcccc attaaatgta tttaaataat ttgagagcca ttcaacatgc
     2341 tctttatcta aaaagtttgt tggttcgtct aataatagaa catcatggtt agaaagtagt
     2401 aattttgcta aaattacctt tgccctttga ccaccactaa gctctctaac aacacgattc
     2461 attcctattg aatctaatcc aagaccattt gctattttat ttatttcatt atctatagaa
     2521 tagaaattac ttgctattag ttgttcctga taatctgatg ctttgattag atattgctca
     2581 ttttcagata tagcactttt ttgatagagt aaattcattt ccttttctat tttataaagt
     2641 tcttcaaatg cagtgtgtaa atagagtgat attgttgtat ctctattgat ttctgcatat
     2701 tggtctaaat ggcctatatt gatattaggt tgccacttga tttctcctga atctggaaca
     2761 acttctccca ataaaatttt aattaatgta cttttacctg taccattttg gccaactaca
     2821 cctatatgtt cacctttgaa taaatcaaag ttagctttct catacagtga tttatccata
     2881 aaacattgtg ataaattagt tacttttaat aaactcataa ttcaaactcc ttttctttta
     2941 ttaaaaaatt tatggaaata aaaaaagcag aggaatggca ttgtaaaatg ccgacctctg
     3001 cttttggaaa aacaaaaggt catagacaag cattgttgtc tatgaccaga aaatctatat
     3061 tatctatcgg caaataggta attttaaata agcatagcca aaaaactata ctaaacctat
     3121 atttgccgat gaagatagaa agctttaagc tttcagatta acctctacac aacgcttcat
     3181 cgtatacatt tgtgtagagt taagttttct aacaagttta actaattcta tataaatcat
     3241 aattattcac atcctaatct tagtattaat tctagtttat ctgtatttaa attgtacttt
     3301 tgtactttgt ggattatctt atttatttaa aatatgtttt attaattata gcttaagtta
     3361 tgttatcata atacttagtt atagtcaata agaaagttat atttactatt attatattaa
     3421 tttagtaaat ctatcattac aaagttattt gaagactcgc tatcaataaa attttatagt
     3481 ctataagaaa attaagatta tgagttttat aattttgata tagggatact attttc
//
LOCUS       DAEPIN010000163         3444 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.166, whole genome shotgun sequence.
ACCESSION   DAEPIN010000163 DAEPIN010000000
VERSION     DAEPIN010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3444
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.166"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            complement(<1..199)
                     /locus_tag="KR250_003752"
     CDS             complement(<1..199)
                     /locus_tag="KR250_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HBH0069630.1"
                     /translation="MNFSNIFIQVAVLFIIILVGYFVRKFNLLDDHCTSKLSTLTMTV
                     FLPSMIISSMQINFDSKMIQKI"
     gene            389..2260
                     /locus_tag="KR250_003753"
     CDS             389..2260
                     /locus_tag="KR250_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HBH0069631.1"
                     /translation="MKNTKFSIKKIKNISNDPKVVHTKGALLEKMGKMESAIELYKSA
                     ALDNYVKSQYTLGNIYESKKQFKEAEKWYSMAYKSGSEDAAFDLGNMYYKLDGYEYAI
                     YWYEKIANLGYLQAQNNLGVCHFKMKDFVRCEKWLKTAADKNLGKACFNLGVLYTFLE
                     KDDEAYEYYKKGSVLLDDDAKYNLAILNGKKKDNKSTVSLYKQLYKSGHMKGCFNLGM
                     IMEINDNLEDAEKYYKKSADRDDVKSEYRLAYVYDRKEELGDAIYYYEKAIEKNHTLS
                     KYRLANLYNRENEIEKAKTYYKMAAEDDITEAKNNLAGIYFEEKDYENAIKYYEDAIA
                     VGCKSSLENLGDLYYQNQDIEKAISYYSRIPNNVSCQIKLGNIYEDLNNIEEAISWYK
                     KASENGDTRSSYRLGCIYESLGNTKNARKYFEMASSKNHMNARIHLGRIYFREGKLEE
                     SKMMFDTPANENNVYAQHMVGLIYDMFYKDYVNSKFWYEKARAQGCVESIYNLGQIYL
                     KLNDDAEAEKYYKEGIKYGSKKCEYMLAGLYYKKSLDMYISLANEEYDNCEEIVEGFP
                     NLNIDFDEVLIPPFKLQEEVIDEEEYVPIYILNIKESLDSMLEGLETEMIIDEEHDS"
     gene            complement(2352..3287)
                     /locus_tag="KR250_003754"
     CDS             complement(2352..3287)
                     /locus_tag="KR250_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HBH0069632.1"
                     /translation="MIRYYKTIDSKLEKLSFFEDGCWINLVEPNHSEINEISNLLNID
                     VESIESALDEEERSRIDVEDNHTLILIDIPVDESDSNSSHYTTIPLGIILTEEAIVTV
                     CDAQTKILNDFIVGHIKDFFTFKKTRFLLQILHKNAAYYLHYLRKINKMTIIIEREIY
                     KSMKNKELVQLLELEKSLVYFSTSLKSNELVLNKMVRTAGIKKYPDDEDLLEDVIVEN
                     RQALDMAKIYGDILSRIMDAFSAIISNNQNNVMQILTVVTLIFSIPTIISGFFGMNVI
                     NMPFSNNPNGFWIILLITSIICIVITFFMSRNKLL"
BASE COUNT         1345 a          360 c          574 g         1165 t
ORIGIN      
        1 gtattttttg tatcatttta ctatcaaagt taatttgcat ggagctaatt atcattgatg
       61 gtaaaaaaac tgtcattgtc aaagttgaaa gcttagatgt gcagtggtca tctaataagt
      121 tgaattttct gacaaaatat ccaactaaaa taattataaa taaaactgca acttggataa
      181 aaatattgct gaaattcata aaatgtcccc cctatatata cattatatac caattacata
      241 ataaaatcta atgattataa aatttaaaat ttaatgttac aatatagtga caaaaggtaa
      301 aaattcaata ttatagcatt aattgtagat tataatgtat aataatgttt gagagaatat
      361 attttataaa atgggtaggt agcgaataat gaaaaataca aaattttcta taaaaaaaat
      421 aaaaaatata tctaatgacc cgaaagtcgt acatactaag ggtgccctcc ttgaaaagat
      481 gggtaagatg gaaagcgcaa tagagttgta taagagtgct gcattggata attacgtaaa
      541 atctcaatat actttaggaa atatatatga aagtaaaaaa caatttaaag aagcagaaaa
      601 gtggtattca atggcatata aaagtggaag tgaagatgct gcttttgatt taggtaatat
      661 gtactataag ttagatggct atgaatatgc tatatattgg tatgaaaaga tagcaaattt
      721 aggttattta caagcacaaa ataatttagg tgtatgccat tttaaaatga aagattttgt
      781 aaggtgtgaa aaatggttaa aaacagcagc agacaagaat ttaggtaaag cttgttttaa
      841 tttaggggta ttatatactt ttttggagaa agatgatgaa gcatatgaat actataaaaa
      901 aggttctgtg cttttagatg atgatgcaaa atataattta gcaatattaa atggaaaaaa
      961 gaaagataat aaatcaacag taagtttata taaacagttg tacaagtcag gtcatatgaa
     1021 aggttgcttt aacttgggta tgattatgga aataaatgat aatttagaag atgcagagaa
     1081 gtattataaa aagagtgctg atagagatga tgtgaagtct gagtataggc ttgcatatgt
     1141 atatgataga aaagaagagc tgggagatgc aatttattat tatgagaaag caatagaaaa
     1201 aaatcataca ctttctaagt atagacttgc taatttatat aatagagaaa atgagataga
     1261 gaaggcaaaa acttattata aaatggctgc tgaagatgat ataacagaag caaaaaataa
     1321 tctggcaggt atatattttg aagaaaaaga ttatgaaaat gctattaaat attatgagga
     1381 tgctatagct gttggatgca aaagttccct tgaaaatcta ggggatttat attatcagaa
     1441 tcaagatata gaaaaagcga tttcgtatta ttcgaggatt ccaaataatg tatcttgtca
     1501 aatcaaatta ggaaatatct atgaagattt aaataatatt gaagaagcaa taagttggta
     1561 taagaaagca tctgaaaatg gagatacgag gtctagttat agattggggt gtatatatga
     1621 gagcttagga aatacaaaaa atgctagaaa gtattttgaa atggcaagta gcaaaaacca
     1681 tatgaatgct cgtatacatc taggtagaat ttattttaga gaaggtaaat tggaagagtc
     1741 taaaatgatg tttgatacac ctgccaatga aaataatgtg tatgctcaac atatggtagg
     1801 attaatttat gatatgttct ataaagatta tgtaaattct aaattttggt atgaaaaggc
     1861 tagagcacaa ggttgtgttg aatcaatata taatttaggt caaatttatt taaaattaaa
     1921 tgatgacgca gaagctgaga aatattataa agaaggtatt aaatatggaa gtaagaagtg
     1981 tgagtatatg cttgcaggtc tttattataa aaagagccta gatatgtaca tttctttagc
     2041 aaatgaagag tacgataatt gtgaagagat agtagaaggc tttccaaatt taaatataga
     2101 ctttgatgaa gtattaattc ctccatttaa gttgcaagaa gaggtaatag atgaagaaga
     2161 gtatgtacca atttatatac taaatataaa agaaagttta gattcaatgc tagaagggct
     2221 tgaaactgaa atgattatag atgaagaaca tgatagttaa aaagtattat aagattatat
     2281 agatttaaat atataaaagc cattaggata aatgtaaatc aattaattct aatggctttt
     2341 ttattaatat tttataatag cttatttctt gacatgaaaa atgtaatcac tatacatatt
     2401 atagaggtaa taagaagtat tatccaaaat ccgtttgggt tattagaaaa tggcatgttg
     2461 atgacattca taccaaagaa tcctgatatt attgtaggta ttgagaaaat tagtgtaact
     2521 actgttaaaa tttgcataac attattctga ttattactta taattgcact gaaagcatcc
     2581 ataattctac tcaatatatc tccatatatt ttagccatat caagagcctg tctattttca
     2641 actataacat cttctaatag gtcttcatca tctggatact tctttatacc agcagttcta
     2701 accattttgt ttaaaacaag ttcattagat tttagtgagg tagagaagta taccaaagat
     2761 ttttctaatt ccaataattg aaccaattct ttgtttttca ttgatttgta aatttctcgc
     2821 tctattatga ttgtcatttt atttattttt cttaagtaat gcaagtaata agctgcattt
     2881 ttgtgaagaa tttgaagcaa aaagcgagtc tttttaaacg taaaaaaatc tttaatatga
     2941 ccaactataa agtcatttaa aatcttagtt tgtgcatcac aaactgttac aatagcttct
     3001 tcagttagta taatacctaa gggtattgta gtataatgag aagaattaga gtcactttcg
     3061 tctactggta tatctataag tataagagta tgattgtctt caacatctat tcttgagcgt
     3121 tcttcttcat ctagtgcaga ttctatgctt tctacatcta tatttaataa attagaaatt
     3181 tcgttgattt cactatgatt aggttcaaca agatttatcc aacagccatc ttcaaaaaaa
     3241 ctaagtttct ctaattttga atctatagtt ttgtaatatc ttatcatggc aacacatcct
     3301 ttattcagtt ttatataaat ctcatcattt aatagtatct attaattaac taatttatat
     3361 aactgaatat atgtattagt tttgatttta gatattatta agtgatgaaa atgtaaaaaa
     3421 ttctctccgc aacggactag tatc
//
LOCUS       DAEPIN010000164         3435 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.167, whole genome shotgun sequence.
ACCESSION   DAEPIN010000164 DAEPIN010000000
VERSION     DAEPIN010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3435
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.167"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            121..1320
                     /locus_tag="KR250_003755"
     CDS             121..1320
                     /locus_tag="KR250_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069633.1"
                     /translation="MKLKSKISILIIFLTLAIYFSIGLLNNEDNLKIKTIKGDSNELD
                     HININFGYALNGYINSYYTINGSNKTKKTELIKLQKDSKNTNNLIDVSSGSDLIAHVG
                     FEMNEESKDINFVIDEKKEDLEKGVFPSEDSDKEIEYEKLDMNNFTRTRYKVDKSTDD
                     YYAKILSSKRYKKYLYVSVLYATSDSSENNKEYSSTIDIIKINPENKKIKTIKSINLS
                     NYFSEKYQNIENIGVSYASKYEDKMYILVNVLNTNIGDNSNSKFHLLEYNLEENKESI
                     MELKTDSKMALAKAKLEANKLYMVMSNDYDNSSITELNLLTYNILDKSLDEDKYVLDN
                     REKRGIRSIEIDSNKIVAYTSRLNSILSWDDKFMLYIINKNSKEIVYEGELQFNSSNS
                     MLEFEIK"
     gene            1329..2141
                     /locus_tag="KR250_003756"
     CDS             1329..2141
                     /locus_tag="KR250_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069634.1"
                     /translation="MKDLIRLFEFELKRNIKNYIFIIICCCSFVILNIVKNLNDYNYI
                     IENAVKSEQLTKIGNTIETVNVTGFSSFRNIMGSTELWFMFGIIACICYAFFIWYRDF
                     NGRSKSIYTLIMLPKNRINIYISKLLNILFLVYSYTVVLTISLFIASKLLPRHMLGNV
                     TNYGFVQETIYELKMLLPYSFEILFVEYIFLLIGFVSVVFTSILINKSIYKVSTLISF
                     LFLVIEILVFIISIEFIIPYEYSDVFTVLYSSINIFVCSLISNKLLKQKIDF"
     gene            2152..3420
                     /locus_tag="KR250_003757"
     CDS             2152..3420
                     /locus_tag="KR250_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069635.1"
                     /translation="MKAKNIGKYTICFIIVCFLMITMTLILSYFNKDKLSVKTLKGNV
                     KDIGRVSVVYTPNISKFSYEEVIISKNGVRNNTNPKSRIRNDYLNIDKKYSDFVNDKG
                     KEFIYKDDLNIGEAYTSVKSDLVQGMNALNDCIKVSNKNLKSGVEENFDINLNKSDFK
                     ENSWKTLFNKIYKGNLYMVISETNISGSKVNNICILKIDIKNKSYKLLNRFNLGLQNG
                     EQQNINEIKFSIGSKLYLEIVNNKSKNGYIEGHDSYSNFIIYDIEDNTFNISNKFMDN
                     LNKEKNANPKEIGLLSKTKLDYMVKNNKLNLIVTDMSNSITNLVYDINSNEIKLEDYE
                     ILDLKVDYNEASSGVRKVKLLDDKIYSISGIKNSISGELKGFKGIVDVVGTKPVKFQV
                     FDMKLKKNIYEAELINGDISLQEKLFFVAN"
BASE COUNT         1472 a          262 c          519 g         1182 t
ORIGIN      
        1 aggatttcaa tttaacaatt tagtagcagg gttattaagt tttttaatta taatgtttat
       61 tctaaatgga atatcttatt tgctaattaa aaagaaagta tctctttaag gaggggtggt
      121 atgaaattaa aatctaaaat atctatactt attatatttt tgactttagc aatatatttt
      181 agtataggtt tattaaataa tgaagataat ttaaagataa aaactataaa aggtgactct
      241 aatgaattag accatataaa cataaatttt ggatatgctt tgaatggata tataaattca
      301 tattatacaa taaatggaag caacaaaact aaaaagactg aattaataaa actacaaaag
      361 gatagtaaga acacaaataa tttaattgat gtatctagtg gttcagattt aattgctcat
      421 gttggatttg aaatgaatga ggagtcaaaa gatataaact ttgtgataga tgagaaaaaa
      481 gaagatttag aaaaaggtgt atttccatca gaagatagtg ataaagaaat tgaatatgaa
      541 aaattagata tgaataattt tacaaggaca agatataaag tagataaaag tacagatgat
      601 tattatgcaa agatactatc atcaaaaaga tataaaaaat acttatatgt atctgtactt
      661 tatgctacaa gtgattcatc agaaaataac aaagaatatt caagtactat agatataata
      721 aaaattaatc cagaaaataa aaaaatcaag actataaaaa gtataaattt aagtaattat
      781 ttttccgaaa aataccaaaa catagagaac ataggtgtaa gttatgctag caaatatgag
      841 gataagatgt atattttagt aaatgtacta aatacaaaca taggtgataa tagtaattct
      901 aaatttcatt tacttgagta taatcttgaa gaaaataagg aaagcataat ggaattaaaa
      961 actgatagca aaatggcttt agcaaaagca aagttagaag ctaataaatt atatatggta
     1021 atgtcgaatg attatgataa tagttcaatt acagagttaa atttacttac ctataatata
     1081 ttagataaaa gcttagatga agataaatat gttttagata atagagaaaa aagaggaatt
     1141 agaagtattg aaattgattc aaataaaatc gttgcatata catcaaggct aaactctata
     1201 ttatcttggg atgataaatt tatgctatat ataataaata aaaattctaa ggaaattgta
     1261 tatgaaggag aattacaatt taacagttca aatagtatgt tagaatttga aataaagtag
     1321 gtgagttgat gaaagattta ataagattat tcgaatttga attaaaaaga aatataaaga
     1381 attatatatt tataataatt tgttgttgtt cttttgtaat tttaaacata gtaaaaaatt
     1441 taaatgacta taattacata atagaaaatg cagttaaaag tgaacagtta acaaaaatcg
     1501 gaaatacgat agaaacggta aatgttacag gtttctcaag ctttagaaat ataatgggtt
     1561 ctacagaatt gtggtttatg tttggtataa ttgcatgtat ttgctatgct ttttttattt
     1621 ggtatagaga ctttaatggt agaagtaaat ctatatatac acttataatg ctacctaaaa
     1681 atagaatcaa tatttatata tctaaattat taaatatatt atttctagtg tatagctata
     1741 cagtagtact tacaatatct ttgtttattg caagtaagtt gttacctaga catatgcttg
     1801 gtaatgtcac aaactatggt tttgtacaag aaacaatata tgagttaaaa atgttacttc
     1861 catatagctt tgaaatacta tttgtagagt atatattttt attgatagga tttgtatctg
     1921 tagtatttac atcaattctt ataaataaat ctatttataa agtaagcact ttaataagtt
     1981 ttctattttt agttatagaa attttagtgt ttataatttc tatagaattt attatacctt
     2041 acgaatacag tgatgtattt actgttctat attcaagtat aaacatattt gtatgtagtc
     2101 ttatatctaa taaacttttg aagcaaaaga ttgactttta ggagggatat tatgaaagca
     2161 aaaaatatag gaaaatatac aatatgtttt ataatagtat gttttttaat gattactatg
     2221 accttaatat tgagttattt taataaggat aaactatctg taaaaacttt aaaaggcaat
     2281 gtaaaagata tagggagagt aagtgtagta tatacaccta atataagcaa attttcttat
     2341 gaggaggtaa ttataagtaa aaatggagta agaaataata caaacccaaa atcaagaatt
     2401 agaaatgatt atttaaatat tgacaaaaaa tacagtgact ttgttaatga taaaggaaaa
     2461 gaatttatat ataaagatga tttgaatata ggagaagcgt atacctctgt taagtctgat
     2521 ttagtacaag gaatgaatgc tttaaatgat tgtataaagg tttcaaataa aaatttaaaa
     2581 agtggggtag aagaaaattt tgatattaat ttaaataagt ctgattttaa agaaaatagt
     2641 tggaaaacct tatttaacaa aatatataaa ggtaatttat atatggtaat tagtgaaact
     2701 aatatttcag gttctaaagt caataatatt tgtatattaa agatagatat aaaaaataag
     2761 tcttataaat tattaaatag atttaattta ggattacaga atggagaaca acaaaatatc
     2821 aatgaaatta aattttctat tggaagtaaa ttatacttag aaatagtaaa taataaaagt
     2881 aaaaatggat atattgaagg acatgatagt tactctaact ttattatata tgatatagaa
     2941 gataacactt ttaatataag taataaattt atggataatc taaacaaaga aaagaatgca
     3001 aatccaaagg agattggctt attaagtaaa actaagctag attatatggt aaaaaacaat
     3061 aaattgaatc ttatagttac ggatatgagt aattccataa ctaatcttgt ttatgatatt
     3121 aattctaatg agattaaatt agaagattat gagatacttg atttaaaagt agattataat
     3181 gaagcatcat ctggagttag gaaagttaag ctactggatg ataagatata ttctatttca
     3241 ggtattaaga acagtatatc aggagaatta aaaggattta aagggattgt tgatgtagtc
     3301 ggaactaagc cagtaaaatt tcaagtattt gatatgaaac ttaaaaagaa tatttatgaa
     3361 gctgagctaa taaatggaga tataagttta caagaaaagt tattttttgt tgcaaattaa
     3421 gtgttagaat taatt
//
LOCUS       DAEPIN010000165         3380 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.168, whole genome shotgun sequence.
ACCESSION   DAEPIN010000165 DAEPIN010000000
VERSION     DAEPIN010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3380
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.168"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     repeat_region   2..559
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=2..30
                     /rpt_unit_seq="atttacattccatatagttaatataaaac"
     gene            complement(1218..3311)
                     /locus_tag="KR250_003758"
     CDS             complement(1218..3311)
                     /locus_tag="KR250_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBH0069636.1"
                     /translation="MNKHNFEVILNQLQINIYVTNIHTNEIIFMNKKMKEEYNILDPE
                     GKVCWQVLYPEKNSTCSFCKVLELLKNDKKGVLIKWYEKCNKLNRVFENYDSLITWQD
                     GTVVHMHQSIDIANSTSLNKPIKINEFHEISNNKEEKGVFNFSRDNFDYNSTLLYDAL
                     IRGTDEYIYICNMKTGVFRYSPSQVELFDLPGEIVENPLVYWKKIVHPEDWNRFYKSN
                     TEIGKNQMDYHTVEFRAKNRSGEYIWLRCRGQLMRDEFGEPSIFAGIMTQLGKQNKID
                     SLTQLLNYHEFMSVFEDKISNPMIEKLCIVLLDIDDFKNVNEMYDRDFGDNIIKTLAQ
                     SIQSILPDNAELYKLDGDEMGILVDNVEENEILTLYNQIQNMIIHLQLWRKYGLNITI
                     SAGCVIYPKHGDTVKELYKCASYSLQYAKEHGKNRLVFFSQEILKNKMYSLEMMRDLK
                     ASINDDFRGFSLRFQPQVDTESHKIIGVEVLLRWTNDKCKAISPLEFIPILEENDMIN
                     IVGAWVLRMALRTFRKWIDYYPFFKVSVNVSAVQILEDTFIEDIVKIIDDENFPYQNL
                     VLELTESHTVQNMSILQFKFKALQDLGIYIAMDDFGTGYSSLEVLKFSPIDIVKIDRV
                     FVKDILKSKFDATFIHFIVAICHDVGIKVCLEGVETQEEYDLVKQIKPDYIQGYLFGK
                     PQTATEIFDLLKLDN"
BASE COUNT         1173 a          547 c          329 g         1331 t
ORIGIN      
        1 tatttacatt ccatatagtt aatataaaac ctgcttaatg cagaattaag agaggaagtg
       61 ttataagatt tacattccat atagttaata taaaactggt tgttcctgct ccggtgttgg
      121 tggaactggt acatttacat tccatatagt taatataaaa cgaatatgta tggataattt
      181 tttacttaac atattagcat ttacattcca tatagttaat ataaaacctg ctgtaatttc
      241 tgatgaagct tctccactac caccatttac attccatata gttaatataa aacccttctt
      301 taactcttat cgtatttaaa cctcttataa catttacatt ccatatagtt aatataaaac
      361 tttttgtgtt aagaagaaaa aagaaatttt gataatattt acattccata tagttaatat
      421 aaaaccttaa agaaaaaatg gaggaagatt aagtgaaaaa attatttaca ttccatatag
      481 ttaatataaa actggattta aataatcaaa acaactccgg tttaaaaaac atttacattc
      541 catatagtta atataaaacc ccaaaataaa cttagtattt tcaacactta cacgtataca
      601 actctcttaa atttgcagtg aacgagcaat agtgcaattg ataacgctta tcatacactc
      661 tcaacgcctt atatttcaac tgttacgcca aattttttca caaatatcgc tcactgcaaa
      721 attcctataa ttttattata tcataaatat attacttttg aatattgata ccaatttata
      781 ccataataaa agcaaaaaaa cgtaatctat ctacattaga tttaaattct ataacttaaa
      841 gttttttaca taatatttat gcttgtttaa tcaatctaat atatcattct tgtatattgc
      901 ttttaatagt cttttttctt aaataaatat ctatctgaac aagcatagtt taattaaaaa
      961 cttttgtatt cccttaaaac tgtatctacc tcttattatt ttatttatga tatattattt
     1021 ttaatctaaa aatagatatc tatttttatt atggttatcc ctttttaaaa tactttaatc
     1081 atatactatt cccaatttaa catccattga aatgctattt tcttatattg caatttataa
     1141 ttgatatatc ttcaatataa taatttaatg tatttttgat tttaaagctt ttcagtgata
     1201 ttattgataa attagaatta attatctagc tttaaaaggt caaagatttc tgttgctgtc
     1261 tgcggttttc caaataaata tccttgtatg taatctggct ttatttgttt aactaaatca
     1321 tactcttctt gtgtttctac accttctaaa catactttta ttcctacatc atgacaaatt
     1381 gcaacaataa aatgtataaa tgtagcatca aacttactct ttaatatatc tttaacaaat
     1441 actctatcaa ttttcactat atctattgga gaaaatttta aaacttctag agacgagtat
     1501 ccagtaccaa aatcatccat agcaatataa attcctaaat cttgtaaagc cttaaattta
     1561 aactgtaaaa tactcatatt ttgtactgta tgactttctg tcaattctaa aacaagattt
     1621 tgataaggaa aattttcatc atctataatt tttactatat cttcaataaa tgtgtcttct
     1681 aatatttgaa cagctgaaac gtttacactc actttaaaaa aaggatagta atcaatccat
     1741 ttcctaaaag tacgtaatgc cattcgaagc acccaagccc ctacaatatt tatcatatca
     1801 ttttcttcta atattggaat aaattccaaa ggagaaattg ctttgcattt gtcattagtc
     1861 caccttaaaa gaacttctac acctattatt ttatgacttt ctgtatctac ttgtggctgg
     1921 aatcgaagac taaaccccct aaaatcatca ttaattgatg cctttaaatc ccgcatcatt
     1981 tctaatgaat acattttatt ttttaatatt tcttgggaga aaaaaacaag cctgttttta
     2041 ccatgctctt tcgcatattg taaagagtaa ctagcacact tataaagttc ttttactgta
     2101 tctccatgtt ttggatatat tacacaacca gcagaaattg ttatatttaa tccatatttt
     2161 ctccatagtt gaaggtgtat aatcatattc tgtatttgat tatatagagt tagaatttca
     2221 ttttcttcaa cattatcaac aagaatgccc atttcgtccc catcaagttt ataaagttca
     2281 gcattatctg gtaaaataga ttgtatagac tgtgctagtg tttttattat attatctcca
     2341 aaatccctat catacatctc attaacattt ttaaaatcgt caatatctaa taatactatg
     2401 cataattttt caatcatagg atttgaaatt ttatcttcaa atacactcat aaattcatga
     2461 taattcagta actgtgtcaa tgaatcaatc ttattttgtt tacctaattg cgtcattatt
     2521 cctgcaaaaa tacttggttc tccaaattca tcacgcatta gttgacctct acatcttaac
     2581 catatatatt ctccactacg atttttagcc ctaaattcca ctgtatgata atccatctgg
     2641 tttttaccta tttcagtgtt tgacttataa aaacgattcc agtcttctgg atgtacaatt
     2701 tttttccagt aaacaagcgg attttcaaca atttctcctg gtaagtcaaa taattcaact
     2761 tgtgatggtg aatatcgaaa tacgcctgtt ttcatattgc aaatgtatat atattcatct
     2821 gtacctctaa tcaaagcatc ataaagcaat gtagaattat aatcaaaatt gtctcttgaa
     2881 aaattaaaaa ctcctttttc ttctttatta tttgatattt catgaaattc attaatctta
     2941 attggtttat ttaagcttgt tgagtttgca atatctattg attgatgcat atgaacaact
     3001 gtgccatctt gccatgtaat taaactatca taattttcaa aaacacgatt taacttatta
     3061 catttttcat accactttat taatactcct tttttatcat tctttaataa ttctaaaact
     3121 ttacaaaaac tacaagtaga atttttttca ggatataata cttgccaaca aacttttcct
     3181 tctggatcta aaatattata ttcttctttc atttttttgt tcataaaaat aatttcatta
     3241 gtatgaatat ttgtaacata tatattaatc tgtagttgat ttaatataac ttcaaaatta
     3301 tgtttgttca tatacttatc tcctccatag ttactgctct ttataacaac attgttaaaa
     3361 ttattctttt tagaagattt
//
LOCUS       DAEPIN010000166         3375 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.169, whole genome shotgun sequence.
ACCESSION   DAEPIN010000166 DAEPIN010000000
VERSION     DAEPIN010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3375
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.169"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            complement(225..608)
                     /locus_tag="KR250_003759"
                     /pseudo
     CDS             complement(225..608)
                     /locus_tag="KR250_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431032.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
     regulatory      834..931
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            986..1162
                     /locus_tag="KR250_003760"
     CDS             986..1162
                     /locus_tag="KR250_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069637.1"
                     /translation="MFGNKMDKCTHVLTAYISSSYDYCNFLDTQLDDFILEYGENVVE
                     SCLHQVMVLVSKYN"
     gene            1388..1588
                     /locus_tag="KR250_003761"
     CDS             1388..1588
                     /locus_tag="KR250_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003723180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBH0069638.1"
                     /translation="MNNGIVKWFNNEKGFGFISMEGRDDVFAHFSAIQTSGFKSLEEG
                     QQVSFDIVKGARGPQAENITIL"
     gene            complement(1752..1964)
                     /locus_tag="KR250_003762"
     CDS             complement(1752..1964)
                     /locus_tag="KR250_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069639.1"
                     /translation="MVCKIFITLKTILPKTQIIVTTHSPHILQIDSKEEMIVLDMAES
                     DNVYKKELKLGEYGVLGWTNEGLYLH"
     gene            2339..2521
                     /locus_tag="KR250_003763"
     CDS             2339..2521
                     /locus_tag="KR250_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HBH0069640.1"
                     /translation="MFKFYNNATLMFLPGTNIKTISERLGHADIKITMNSYSQFLEEM
                     DREAFENLSKILFIVF"
     gene            2592..>3375
                     /locus_tag="KR250_003764"
     CDS             2592..>3375
                     /locus_tag="KR250_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069641.1"
                     /translation="MNIIKSITLLLTLLIVILMTGCSPRESASEVAKKYIDKMNSGDY
                     ETAYSLLSKDSKKKISIENLEEFQNILVSTKRIISYKIGKEKIIKKYKHDGKEYKNVV
                     KLEETFNIKNLLYKKDDSLKVNRYFVVENNKYKLLLYESFKKEFGINSMDLAQLKLND
                     FGLKYFKEVDLILKKAIAINPTDSKLYYAQACNYMNFDGFPGYSDTTYNALDSINKSL
                     KYLDKNDSDYKKNASNIYNLKGVILMFHKRFDEAQQYLNKALY"
BASE COUNT         1259 a          408 c          428 g         1280 t
ORIGIN      
        1 gtcaatataa aagacagtaa tttatctata gtgttactta tattttatat atatggctat
       61 ttttcaacat tttacaatat ataacattta tttttttaac ttttgtcagt aaaatgtcag
      121 caaaacactt tatccacaat ttttatataa gttttccaca acttatcacc aatattatcc
      181 acataaaaaa ggtagcactt atattattag ctacctttta tttattaatc tttatatttc
      241 cagcttttta tccaacttcc tatttctatt attttttctt cagttatttc ttctccatca
      301 tgtaattttg aaattaattc tgataaagga ttgttatgta aacctctaaa aactcttttt
      361 ccctctattt tgagatattc tctctctttt attaatactg tatagtgtac atgcctccca
      421 attctctatg atattaaaaa accttttttg gttaatctag ctaaaagtat aattgtagtt
      481 gtatgcttcc aaccatatgt tttttctatt tcatctgcaa catcttttga tattacagtt
      541 ttataaccag tatgccatat gcatttcata atttttaatt ctgtatctgg taatttttta
      601 tttagcataa tatattcctt ctttcaattt atgtcacttc ttcaaattta tctagcttaa
      661 attataatag gagcttttta gaacaaaaac tatatttaat ttttaccagg tactctacgt
      721 gtttagtctg tttttaagtg taaaataatg ttcatatcta taaatttatt taactttcaa
      781 acaatcctgt tgaataataa ctaattctat tgtattattt tagttgtaga ataaaactaa
      841 atcggcaaaa ctagagaaat ttagtgacgc aaagctatag ggactaagac ttatataaat
      901 atcttatgag ttatgtcagc cagttgccaa agagatagtg ttctttttgt ttttatgaaa
      961 gttttttagg gggataataa tttatatgtt tggaaataaa atggataaat gtacacatgt
     1021 attgactgct tatattagca gttcgtatga ttattgtaat tttttagata cacagttaga
     1081 tgattttata ttagagtacg gagaaaatgt agtagagtct tgtttacacc aagtaatggt
     1141 attggtaagt aagtataatt agatagacat tgatttctag aaaacccttt ttagttttat
     1201 ctacatttat attaatatat agcaatgtaa aacgtttaaa tatattttta atattaagaa
     1261 taattaaaat gttaactaaa aaatataatt aagtagacat ttctattaat ctatgataca
     1321 atagtatttg tggtaataca caattaaatc aagtatatct tgatataggt taataggagt
     1381 atttttaatg aataatggaa tagtgaaatg gtttaataat gaaaaaggat tcggttttat
     1441 atcaatggaa ggtagagatg atgtgtttgc tcatttctca gctatacaaa cttcaggatt
     1501 taaatcatta gaagaaggtc aacaagtaag ctttgatata gttaaaggtg ctagaggtcc
     1561 tcaagcagaa aatataacta tattataata tatagatttt tcgggaaaga ctctttttag
     1621 agtctttttt tattgttaat atatgtaaga taatcattgg tgaagtaaat aaggtatatt
     1681 gagtaatgtt cttgatagtt tatatgctaa tgaatttcaa aaaaataagc actcctataa
     1741 aaagagtgct cttaatgtaa atataaacct tcattggtcc agcctaatac cccgtattct
     1801 ccaagtttta actctttttt atatacatta tcactttcag ccatatctaa cacaatcatt
     1861 tcttctttag aatcaatttg taaaatatga ggactgtgtg ttgtgacaat tatttgtgtt
     1921 tttgggagaa ttgtttttaa tgtaataaaa attttacata ccacatagtt gatataaaac
     1981 ttaagactag cagactcata agcaattagt tgcctctctt tacataccac atagttgata
     2041 taaaacctag aggctctcag agaagttata agatgtttag taactttaca taccacatag
     2101 ttgatataaa actccaaaat aaacttagca tctccaatgt ttacacatat acaatgctct
     2161 taaaattgca gtgaacgcgc aatattgcaa ttgataatac ttatcatata ttctcaatac
     2221 cttatatttc aactgctaaa cctcaattta tcacaaatat cgctcactgc aaaatttcta
     2281 tatttttatt atatcatagc atataattaa tattttcaat taatttttac atttaattat
     2341 gtttaaattc tataataatg caactttaat gtttcttcct ggaactaata taaaaactat
     2401 ttcagaaaga ttaggtcatg cagatataaa aataactatg aatagctact ctcagttttt
     2461 agaagagatg gacagagaag catttgaaaa tttgagcaaa atactattta ttgtatttta
     2521 aagaagatga cttattcaat atttgatgct agaatataat taataaaaat ttaattatag
     2581 gtgattgtat tatgaatatt attaaaagca taactttact cttaacatta ttaattgtga
     2641 tattaatgac aggctgttcc ccaagagaaa gtgctagtga agttgcaaaa aaatatattg
     2701 ataagatgaa ctctggtgac tatgaaacag catatagttt attaagtaaa gatagtaaaa
     2761 aaaaaattag tatagaaaat cttgaagaat ttcaaaatat tttagttagt acaaaaagaa
     2821 taattagtta taaaataggt aaagaaaaaa tcataaaaaa atataaacat gatggaaaag
     2881 aatataaaaa tgttgttaaa ttagaagaaa cttttaatat aaaaaacctt ctttataaaa
     2941 aagatgattc tctaaaagta aatagatatt ttgtagttga aaataacaaa tacaagctat
     3001 tattgtatga aagtttcaaa aaagaatttg gtattaatag tatggacctt gctcaactta
     3061 agcttaatga ttttggacta aaatacttta aagaagttga ccttatacta aaaaaagcta
     3121 ttgctattaa tcctactgat tcaaagcttt attatgccca agcatgtaat tatatgaatt
     3181 ttgatggatt tcctggttat tcagatacaa catataatgc tttagattca attaataaat
     3241 ctttaaagta tttagataaa aatgattctg actacaaaaa aaatgcaagt aatatatata
     3301 acttaaaagg agttattctt atgtttcaca aaagatttga tgaagcacaa caatatttaa
     3361 ataaagcctt atact
//
LOCUS       DAEPIN010000167         3079 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.170, whole genome shotgun sequence.
ACCESSION   DAEPIN010000167 DAEPIN010000000
VERSION     DAEPIN010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3079
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.170"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            228..1670
                     /locus_tag="KR250_003765"
     CDS             228..1670
                     /locus_tag="KR250_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HBH0069642.1"
                     /translation="MRTIRRVLALGLTLAIFLINVPNVDALTSDTIKGNNIYETAGLI
                     ADKKSYDTAIMVNMDNSIADGLSASGLAGAVDAPILLAQKNKIPNETKQRLKNVKKIY
                     IIGKELSISKSVETELKNTGAQVTRLGGDDRIKTSYSVAKEVNGIKKVDEVILTNAYK
                     GEADTISAAPVSVRDIAPIVLTDGKSVPFSTSNVKTYAVGGSISMSTSLVNKTNAKRL
                     GGSDRYDTNKKVIKEFYPDASEFYLSDGYDLVNALTGSTIAKENPIVLVSESSDKSIL
                     AGADKITRLGSISDSVYNKCVSAAQNNGDSSTKGESPMKNETSILGQPTASLEACLKW
                     AKSKKANDLFIELIPILYDTAVQEGVNPVLAVAQSAKETGFCNFGGVLDASFKNPCGL
                     KTSVGGSDTDKNAHSRFDTWEEGILAQIQHLCLYAGQDGYPLSNPVDPRHEKSLFGKA
                     KTVESLSNNWAGGQYGQDLVRMMGEIEATK"
     gene            1712..2065
                     /locus_tag="KR250_003766"
     CDS             1712..2065
                     /locus_tag="KR250_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069643.1"
                     /translation="MKKSNIFSIIFPTVIMVLMFLTFATDIFNIPDIQSKSIFVVGMV
                     LIFPISFLIQGIICVLNKTNWILSLIVSLVTYIVLMYIFLNDSAYIYVLLYTAFYMIG
                     HITAKLYYKIKTFKN"
     gene            2256..3077
                     /locus_tag="KR250_003767"
     CDS             2256..3077
                     /locus_tag="KR250_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HBH0069644.1"
                     /translation="MNLIDNHIHTNFSSDGKDSMEDTIKKAISIGVRYLTFTDHLEHD
                     EDRGFCINYNDYVPVFNKFKEKYKKDIELLLGVEVGYRKHLKNEIEEIINSNPFDFVL
                     CSTHTIDNVPVPSKEYFKGLSKEDAYYKYFNSILETNREFGDYNIYGHLDYISRYGIY
                     SDNRVIYNDFKDVIDEVLKSIINNGSGIELNTSGYRYGLNAIHPNEDILKRYKELGGF
                     IVTVGSDSHRVEDICKDFDVAYDMLKYLDFKYVSLFKERETYFVNIEKVKSNNIA"
BASE COUNT         1194 a          318 c          541 g         1026 t
ORIGIN      
        1 attacaaatt gttactaaat ataggtacat tagaacaaaa atctagtaaa ttgtaactaa
       61 aatgtaaatt tttcctttaa tctatacaat attatggaaa ctttttgtaa tttatgatat
      121 aataacaaat atataattat taaattataa taaatgacta tagcgtagat gtaatactat
      181 gttatagtca tttttgaatt aaacttgata tatcaagggg gaagaatatg agaacaatta
      241 gaagagtttt agcattaggt ttaacattag ctatatttct aataaatgta cctaatgtag
      301 atgcattaac atcagataca attaaaggta acaatatata tgagacggct ggattaatag
      361 cagataaaaa gagttatgat acagcaatta tggtaaatat ggataattct atagcagatg
      421 gtctttcagc aagtggtctt gcaggtgctg ttgatgctcc aattttgctt gcacaaaaaa
      481 ataagatacc aaatgaaaca aaacaaagat taaaaaatgt taaaaagata tacataatag
      541 gtaaagaatt atcaataagt aagtcagtag aaactgaact aaaaaacaca ggggcacagg
      601 taactagatt gggtggagat gacagaataa agactagtta tagtgttgct aaagaggtaa
      661 atggtatcaa aaaagttgat gaagtgatat taactaatgc atataaagga gaagcagata
      721 caataagtgc tgcacctgta tcagtaagag atatagctcc tatagtactt acagatggaa
      781 agagtgtacc tttttcaaca agcaatgtaa aaacatatgc agtaggtggg agtatatcaa
      841 tgagcactag tttagttaat aaaactaatg ctaaaagact tggtggttca gatagatatg
      901 atacaaataa gaaagttata aaagagtttt atccagatgc atcagaattt tatttgagtg
      961 atggatatga tttagtaaat gcacttacag gttctacaat tgctaaggaa aatccaattg
     1021 tattggtgtc agaaagtagt gataagtcta tattagcagg agcagataag attactagat
     1081 taggttcaat aagtgacagt gtgtataata agtgtgtttc tgctgcacaa aataatggcg
     1141 attcgtctac aaaaggtgag tcacctatga aaaatgagac aagcatatta ggtcagccaa
     1201 cagctagttt agaagcgtgc ttaaaatggg caaaatctaa aaaagctaat gatttattta
     1261 tagagttaat accaatatta tatgatactg ctgttcagga aggtgttaat cctgttttgg
     1321 cagtagctca atctgcaaaa gaaactggtt tttgtaattt tggtggagta ttagatgcat
     1381 catttaagaa tccttgtgga cttaaaactt ctgtaggagg ttctgatact gataagaatg
     1441 cccattcaag atttgatact tgggaagaag gaatattagc tcaaattcaa catttatgct
     1501 tatatgcagg acaagatggt tacccacttt caaatccagt agaccctaga catgaaaaat
     1561 ctttatttgg taaggctaaa acagttgaaa gtttatctaa taattgggct ggaggtcaat
     1621 atggacaaga tttagtaaga atgatgggag aaattgaggc aacaaaataa ttgaagatta
     1681 tcaaatatcc aataaataag gattgattat tatgaaaaag agtaatattt ttagtatcat
     1741 atttcctaca gtcataatgg tgctaatgtt tttaactttt gctacagata tatttaatat
     1801 tccagatatt caatctaaaa gtatttttgt tgtgggaatg gttttgattt ttccaatttc
     1861 ttttttgata caagggataa tttgtgtatt aaataagact aattggattt tatctcttat
     1921 tgtgtcttta gtaacttata tagtattaat gtatatattt ttaaatgatt ctgcatatat
     1981 atatgtttta ctttatacag ctttttatat gattggacat ataacagcta aactctatta
     2041 caaaattaag acttttaaaa attaaattga agtatataaa agtaacatta ggagaagtat
     2101 ccttaatgat tttctaaaat cagaaatagg tgcttctctt ttttgtaaaa ataaaagtat
     2161 gtaaataaaa tttaaccatt ataattttct tttaacaaat gtatacaata aacttgttgt
     2221 ataattaaga tagataaaat aaagggaggg ataatatgaa tttaatagat aatcatattc
     2281 atactaattt ttcaagtgat ggaaaggatt ccatggaaga tacaataaaa aaagctataa
     2341 gtattggtgt tagatatttg acttttacag accatttaga acatgatgaa gatagaggat
     2401 tttgcataaa ttataatgac tatgttccag tatttaataa gtttaaagaa aaatataaaa
     2461 aggacataga gttattacta ggtgttgaag tgggttacag aaaacatcta aaaaatgaaa
     2521 tagaagaaat aataaattct aatccttttg attttgtatt gtgctcaact catacaattg
     2581 acaatgtacc tgttccatca aaggagtatt ttaaaggtct tagcaaagaa gatgcgtatt
     2641 ataaatattt taatagcata cttgaaacta atcgtgaatt tggagattac aatatatatg
     2701 gtcatttgga ttatatatct aggtatggaa tatattcaga taatagagtt atatacaatg
     2761 actttaaaga tgttattgat gaggtcttaa agtcaataat caataatggg agtggtatag
     2821 agctaaatac ttctggttat agatatggat taaatgctat acatccaaat gaagatattc
     2881 taaaaagata taaagaactt ggaggattta tagttactgt aggttctgat tctcataggg
     2941 tagaagatat atgtaaagat tttgatgtgg cctatgatat gcttaagtat cttgacttta
     3001 aatatgtatc tttatttaaa gaaagagaaa cttattttgt aaatatagaa aaagttaaat
     3061 ctaacaatat agcttagag
//
LOCUS       DAEPIN010000168         3071 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.171, whole genome shotgun sequence.
ACCESSION   DAEPIN010000168 DAEPIN010000000
VERSION     DAEPIN010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3071
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.171"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     repeat_region   6..1155
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=72..100
                     /rpt_unit_seq="atttacataccacatagttaatataaaac"
     gene            complement(1351..1590)
                     /locus_tag="KR250_003768"
     CDS             complement(1351..1590)
                     /locus_tag="KR250_003768"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069645.1"
                     /translation="MECYLKRTLIIFSEAFLSISFKTRKYLFIVIFILVCPNLSEMIF
                     ILVPARRRIIRIDNIVSIYKYVGVLKLINYSILIL"
     gene            1802..>3071
                     /locus_tag="KR250_003769"
     CDS             1802..>3071
                     /locus_tag="KR250_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBH0069646.1"
                     /translation="MLKVLKGNIIFTKTSETFTVFENSYIILEKGKVKGIFKEIPKEY
                     KNLKVVDYTDKLIIPGMNDLHAHASQFKNLGMAMDKELLPWLETYTFPEESNYKDIEY
                     ATKMYKKFVKELWKSGTTRIAVFATVHKEASMKLMDIFKEVGLGAYVGKVNMNMNCPD
                     TLLENTQDSITDTEEIINKYSDTDSIVKPIITPRFIPSCTSELLKGLGDLCLKYNIPI
                     QSHLSENYDEIEWVRSLEPESKFYGDAYNRYNLFGQTPTLMAHCVHCSQDEIDLMREN
                     NVMAVHCPASNFNVGSGAMPIRKLIDNNIRLALGSDISGGHTLSIFKAMVSAIQLSKL
                     YWVNSGKKYNFLSLSEAFYIATKSGGSFFGKVGSFEEGYDFDALIIDDSNLNHDNYSI
                     LERLERFIYVGDDRNIIHRYVCGKLIEEPNI"
BASE COUNT         1215 a          418 c          394 g         1044 t
ORIGIN      
        1 tgcaaattta cataccacgt agttaatata aaacaagatg tttttaaaaa taaacttaat
       61 atagatattg aatttacata ccacatagtt aatataaaac tttaatcata tatcagatag
      121 gtcacaactt aagagtgatt tacataccac atagctaata taaaacaact tctcatattc
      181 cagataattg ttattttacg aaatttacat accacatagt taatataaaa cctgtagatt
      241 tgcctgtttc gatattgctc atgtgtgaat ttacatacca catagttaat ataaaactct
      301 cttgtttctt ctgttaattt tattatacct tcattattta cataccacat agttaatata
      361 aaacgcgtta tattgttgta taaaaccgat taaagttcaa tttacatacc acatagttaa
      421 tataaaactt gcaacatatt gttttatcgt tgctgggtct ttaaaaccat ttacatacca
      481 catagttaat ataaaactaa gcgatattag agagcgcaag gcatagcgga gcaaatttac
      541 ataccacata gttaatataa aactttaatt ttaaccaccc cctttcacta ataaaaaaag
      601 atttacatac cacatagtta atataaaaca agacgctata caagaggtaa tttgaaagca
      661 atctaattta cataccacat agttaatata aaacgttgca tatgtatgtc taagagcatg
      721 aaatttaatt ttatttacat accacatagt taatataaaa cttctattaa gtgtaagcaa
      781 aacgcttgga atatgaaatt tacataccac atagttaata taaaacttat acacaaatac
      841 aacttgaaga agcgctaaag agatttacat accacatagt taatataaaa cctaatgagt
      901 acatagataa acgtacagga gaggttaatt tacataccac atagttaata taaaacgttg
      961 ataattttcg tttgtatact ttttataatt taaaatttac ataccacata gttaatataa
     1021 aacataaaga tttagaaaaa gaaaaagtta aaattttgga atttacatac cacatagtta
     1081 atataaaact tttcctgtta agttgatgtt tgcaacttta actgctattt acataccaca
     1141 tagttaatat aaaaccccaa aataaactaa gtattttcag tacttacaca catacaactc
     1201 tctcaaattt gcagtgaacc atgagtagtg caattgataa catttatcac acaccctcaa
     1261 tcccttacat tgcaactgtt aaaccctact ttatcaaaaa tatcgctcac tgcaaaattt
     1321 atatattttt attatatcat aaaattcaaa ctataaaatt aaaatggaat aatttatcaa
     1381 tttaagaaca cctacatatt tgtaaatact tactatatta tctattctaa taattcttct
     1441 tctagctgga actaatataa aaatcatctc tgaaagatta ggacatacta atataaaaat
     1501 aactatgaat agatattttc ttgttttaaa cgatatggat aaaaaagctt ctgaaaatat
     1561 aatcaaagtt ctctttaaat aacattccat tttatgtaag taggcattat atttttctta
     1621 aatgtcactt aaatgtcagt tatatataat aatagtaaac aactgtattt cactaatatt
     1681 tgctattatc taatagtatt tatagtttag atttgatata atacatgtgg taaaattttg
     1741 atttgtaaaa caaaatttta cctacatata ttaaaacaaa tttaaaacta aggagaccta
     1801 tatgttaaaa gtattaaaag gaaatataat ttttacaaaa acttcagaaa ctttcacagt
     1861 atttgaaaat agttatatta tactggaaaa aggtaaagta aagggaatat ttaaagagat
     1921 acctaaggaa tacaaaaacc taaaagtagt agattacact gataagctta taatacctgg
     1981 tatgaatgac ctccatgccc atgcctctca atttaaaaat cttggaatgg ctatggataa
     2041 agagcttttg ccatggcttg aaacttatac atttccagaa gaatcaaatt ataaagatat
     2101 agaatatgca acaaaaatgt ataaaaaatt tgtaaaagaa ctttggaaat ctggaactac
     2161 aagaatagca gtgtttgcaa cagtacataa agaagcctct atgaaactta tggatatatt
     2221 caaagaagtt ggtcttggag catacgttgg taaagtaaat atgaatatga attgccctga
     2281 tactttatta gaaaacactc aggattctat tactgataca gaagaaatta taaataagta
     2341 tagtgataca gattctattg taaaaccaat aataacacct agatttatac caagctgtac
     2401 aagtgaactt ttaaaaggtt taggagattt atgtttaaaa tataatattc ctattcaatc
     2461 acacttatct gaaaattatg atgagattga atgggttaga agtctagaac ctgaatctaa
     2521 attctatgga gatgcatata atagatataa tttatttggt caaactccta ctttaatggc
     2581 tcattgtgtt cattgttctc aggatgaaat agatttaatg agagaaaata atgttatggc
     2641 agttcactgt ccagcttcta actttaatgt tggtagtggt gctatgccta tcagaaaatt
     2701 aatcgataat aatattagat tagcacttgg atcagatata agcggtggac atacattgtc
     2761 aatatttaaa gcaatggtat ctgcaataca attatctaaa ttatattggg ttaattctgg
     2821 taagaagtat aatttcttat ctttatctga ggctttttat attgcaacca aaagtggtgg
     2881 aagtttcttt ggaaaagttg gtagctttga agaaggttat gattttgatg cattgattat
     2941 tgatgattca aatttaaatc atgacaatta ttctatactt gaaagacttg aaagatttat
     3001 ctatgttggt gatgatagaa atatcattca tagatatgta tgtggaaaac taattgaaga
     3061 acctaatata t
//
LOCUS       DAEPIN010000169         3069 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.172, whole genome shotgun sequence.
ACCESSION   DAEPIN010000169 DAEPIN010000000
VERSION     DAEPIN010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3069
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.172"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            242..580
                     /locus_tag="KR250_003770"
     CDS             242..580
                     /locus_tag="KR250_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0069647.1"
                     /translation="MANIGKIIGDRIKVYRTKLGYSQEFLAEKAGLHPTYIGQIERAE
                     TNTSINIIMKIATALDIPLELLFANIITTEGVDNSVPLECYEMINKLTEKEQIAMLEL
                     IKCIIKYKEI"
     gene            602..919
                     /locus_tag="KR250_003771"
     CDS             602..919
                     /locus_tag="KR250_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0069648.1"
                     /translation="MNYIGKRLKEERKKANLTSKEFANMVGVSPWYITQIESGKKNPS
                     LKTFIKFVNILNISADVLIKDITSTGKTYLENDINEELKDLNSRELNLISQIIDIIKK
                     NKI"
     gene            1069..1746
                     /locus_tag="KR250_003772"
     CDS             1069..1746
                     /locus_tag="KR250_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069649.1"
                     /translation="MIVIEGSDKFKIAKEYIDVEYTLFSKVTFRYEKLKFKDNAELEK
                     IKMFKYKNGYIPNKLNLSFGYGFSSYKKQIIRETVDTLRLTEIFSSENIEDIKFIKDG
                     TKKLEISIEKVVKFKRRKKRNYVCCYCPDMYRDIKLDKESINKIYNRKIKIEREVNIF
                     EDEDVIINKKVLKFPKSWTKNMQKYWLSENKYPIHSTVIDDDRYKCCNVQYTKNRVII
                     LYYIYNH"
     gene            complement(2074..2841)
                     /locus_tag="KR250_003773"
     CDS             complement(2074..2841)
                     /locus_tag="KR250_003773"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HBH0069650.1"
                     /translation="MKRKVQVKNITIGEGRPKICVPIIGKNKKDIIKEAKELKDACLD
                     IIEWRVDFFENVENIKEVKEVLYELRSYIHDIPLLFTFRSVVEGGEKLISRDYYTTLN
                     KEISNTGLVDLIDVELFMGDEVIDEVVNFAHKKEVKVIISNHDFNKTPKKEEIVSRLC
                     RMQELGADLPKIAVMPQNEKDVLVLLEATNEMFKIYADRPIITMSMSGMGVISRLCGE
                     IFGSALTFGAAKSVSAPGQISFKELNSVLNLLHKSIN"
BASE COUNT         1231 a          396 c          357 g         1085 t
ORIGIN      
        1 aattaacata ttattaattc gttttattat tgtagtatgg ttatgaaatt atgaaaatat
       61 ttctctaatt aaaaatatga tttatccata tttgtttatt gtagatacag actatacaaa
      121 aataaatagc acttatttca gtataaaaac atatatagtc tatatttaga ctaaaaaagt
      181 ataaatagtc tatataaaac aacttaaact atgaatataa tctaaagtgg agggataata
      241 attggcaaat ataggaaaaa taataggaga tagaataaaa gtctatagaa caaaattggg
      301 atatagtcaa gagtttctag cagaaaaggc aggtttacat ccaacatata ttggtcagat
      361 tgaaagagct gagacaaata catctatcaa tattataatg aaaattgcta ctgctttaga
      421 cataccactt gaattgttat ttgcaaatat tattacaaca gaaggcgttg ataactctgt
      481 acctttagaa tgttatgaaa tgattaataa attaactgaa aaagaacaaa ttgcaatgct
      541 agagttaata aagtgcatca ttaaatacaa agaaatataa gtgagggaaa tagtaacaat
      601 aatgaattat ataggtaaaa gacttaaaga agaacggaaa aaagcaaatt taacttcaaa
      661 agaatttgca aatatggttg gagtatcgcc ttggtatata actcaaatcg aaagtggtaa
      721 aaagaatcca agtcttaaaa cattcataaa atttgtaaat atattaaata tttctgcaga
      781 tgttcttata aaagatatta cttccactgg aaaaacatat ttagaaaatg atataaatga
      841 agagttaaaa gacttaaatt caagagaact aaatctgata tcacagatta tagacatcat
      901 aaaaaagaat aaaatataat tctttatatt ttatataaaa aaaaaggtta tttgaaataa
      961 ataacctttt tttgtctttt tgtgttaaca ttgtcttata gtgctaatga gtactattag
     1021 accatgttct atggccttat tttaatataa aacgattgga gataggctat gattgtgatt
     1081 gaaggtagcg ataaatttaa gattgcaaaa gaatatattg atgtagaata tactcttttt
     1141 agcaaagtaa cttttaggta tgaaaagttg aaatttaaag ataatgctga attggaaaaa
     1201 attaagatgt ttaaatataa aaatggctac atccctaata aattaaacct ttcttttgga
     1261 tatggattct cttcttataa aaagcaaata attagagaaa ctgtagatac tttaagattg
     1321 acagaaattt tttcaagcga gaacatagaa gatattaaat ttataaaaga tggtacaaaa
     1381 aaattagaaa ttagcataga gaaagttgtg aaatttaaac gtcgaaaaaa aagaaattat
     1441 gtttgttgct attgccctga tatgtataga gacataaaac tcgacaaaga atctatcaat
     1501 aagatataca acagaaaaat aaaaatagaa agagaagtta atatttttga agatgaggat
     1561 gttataataa acaagaaagt attgaagttt ccaaagtctt ggacaaagaa tatgcaaaaa
     1621 tattggttaa gtgaaaataa gtatcccata cattctactg taattgatga tgatagatat
     1681 aaatgttgta atgtacaata tacaaaaaat agagtgataa tattatatta catatataac
     1741 cattaatata cataattaca aaaaaataat gacaaatata cataatacaa atatatcttc
     1801 tactttagat aaaacagtta ttatgaaaac ttactatcta taaataatag aaatgctagt
     1861 ataccatcta taatctaatg tctagtaggt tttaatggca tattttaaat atgccatagt
     1921 tgatttataa gattttctat atcactttca aaatacacac taaatagttg cttttcagat
     1981 ataattgccc aatttaaatt ttactatctg aaaagttttt tatatttaat ttatatcttc
     2041 aaaaataccg tctaatattt ctatataatt aatttaatta atactcttat gtaatagatt
     2101 aagtactgaa tttaactctt taaatgatat ttgccctggt gctgaaacac tttttgcagc
     2161 tccaaaagtc aatgcagaac caaatatttc accacacaat ctacttataa cacccattcc
     2221 tgacattgac attgttatta ttggtctatc tgcataaatt ttaaacattt catttgtagc
     2281 ttcaagtagc actaacacat ctttttcatt ttgcggcata acagctattt tgggtaaatc
     2341 tgcgcccaat tcctgcattc tacataaacg agatacaatc tcttcttttt ttggagtttt
     2401 attaaaatca tgattagata taattacttt cacttctttt ttatgagcaa aattaactac
     2461 ttcatctatg acctcatctc ccataaataa ctctacatct attaaatcta ctaaacctgt
     2521 attagaaatt tctttattca aagtagtata ataatctctt gatattaatt tttctccccc
     2581 ttctactaca cttctaaatg tgaataataa agggatatca tgtatataac ttcttaattc
     2641 atagagaact tccttaactt ctttaatatt ttctacattc tcaaaaaaat caactcgcca
     2701 ctctataata tccaaacatg catcttttaa ctctttagcc tcttttataa tatctttttt
     2761 atttttccca ataattggta cacaaatttt aggtctacct tctccaatag taatattttt
     2821 tacttgaact tttcttttca tattctcctc cataatcaga aattctatag gactttaaat
     2881 tctattttct gcacaattta attatacatt aaaaatacta tacattataa aaatactaat
     2941 ttaaaaataa tttttattct aattcttaaa acaatcccac atattttaga aaaggtactc
     3001 tataatatac aaacacttct tttttaccta aatttatttt aacatcctat aattttcaca
     3061 atattcttt
//
LOCUS       DAEPIN010000170         3047 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.173, whole genome shotgun sequence.
ACCESSION   DAEPIN010000170 DAEPIN010000000
VERSION     DAEPIN010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3047
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.173"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            499..1164
                     /locus_tag="KR250_003774"
     CDS             499..1164
                     /locus_tag="KR250_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HBH0069651.1"
                     /translation="MIKLIGILIVVIGFILKIDTLFTVLLAGVATGIVAGLDFNQIFT
                     ILGDSFVSNRGVSLFILTLPVIGVLERYGLKQRAVSLIEKLKRLTTGKVLTIYMIARQ
                     IAGALSIRMSGHPQFVRPLVNPMAQAAGLSNSDELKESDEEAIKALSAASENYGNFYG
                     QNLFAGSSGVLLIASTLTQFGYNVTGLNIVKASTIMAVIALVVATIQFTLYDKKLNKS
                     HKK"
     gene            1186..2118
                     /locus_tag="KR250_003775"
     CDS             1186..2118
                     /locus_tag="KR250_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HBH0069652.1"
                     /translation="MQNFINISLEIFYALMGFLMIVIAYKSFTTINNNKKYGTSLFWI
                     LISLPFIFGRLIPANIIGIILILSSLLTLSKQVVFAKYEEPDENFGKEQADKLKNKIF
                     IPSLILAFAAVVVAMSLSNFDNSSQFAIGVGSIIALISALIITKAKPATSVQDGSRLL
                     QQMGPASMLPQLLVALGALFTQAGVGEVISTMISGVVPADSRLFGVIAYVLGMVIFTM
                     IMGNAFAAFSVITAGIGIPFVLSQGGNPAIIGALALTAGYCGTLLTPMAANFNIVPAA
                     LLECKNDYIVIKYQAPVALVLIIAHILVMYFLGF"
     gene            2139..2780
                     /gene="pcp"
                     /locus_tag="KR250_003776"
     CDS             2139..2780
                     /gene="pcp"
                     /locus_tag="KR250_003776"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HBH0069653.1"
                     /translation="MKILLTGFDPFGGEPINPAQEAVERVNNNINGAEIIKITIPTVM
                     TKSVEAIDKAIQEHNPDIVISVGQAGGRFDITPERVAINIDDFRIKDNEGNQVIDTII
                     KEDGEPAYFSKLPVKAMVKHMNENKIPASVSNTAGTFVCNHVMYGILYMIDKKYPNIR
                     GGFIHIPYTTSQVIDKKNTPFMSLEEIVKGLELAIEACIIYKEDVKEIGGEIS"
     gene            complement(2900..>3047)
                     /locus_tag="KR250_003777"
     CDS             complement(2900..>3047)
                     /locus_tag="KR250_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBH0069654.1"
                     /translation="KELEADNLIIRTEYPQIPPKVEYSLSERGKSLIPILDAMCEWGE
                     RNRP"
BASE COUNT         1128 a          416 c          463 g         1040 t
ORIGIN      
        1 ttatcaaaat tttatttttc ttagtgaaat ctaatgaaac tagcacttaa tatatgcttt
       61 attaatcata acaaatatgt tttattaatc ataacaaaaa gagagtgtct aatgaactaa
      121 aaaattcatt agacactctc tttttgtatg aaatcaaata tatttttatc atttcaaaag
      181 tgaaaaatac acttgtctct atgtttggca cattctcttt ttataatata atatctaatt
      241 taaaacctat ttcaaatatt gacatatact taaactatag acttacaaaa tcaattcata
      301 ttagcttaaa atataatctt tgttattcaa attcctatac acaaccatta tttactttga
      361 tactttaaaa catcttccct attttttatt aaattttata ataatagtcc tattaacgca
      421 aatactataa aaagagttta actttatcca ttaaataaat ttaaattaaa acatatagaa
      481 atggaaggag aacaatttat gataaaacta atcggtatat tgatagttgt aattggattt
      541 atacttaaaa ttgacacatt atttactgta ttattagcag gagtggcaac aggaattgtt
      601 gcaggattag attttaatca aatttttaca attttaggag attcttttgt ctcaaacaga
      661 ggtgtatcat tattcatatt aacattgcca gtaatcggtg tattagaaag atatggttta
      721 aaacaaagag ctgtttcttt aatagaaaaa ttaaaaagac ttacaacagg aaaagtactt
      781 actatataca tgatagctag acaaatagct ggtgctctat caataagaat gagtggacat
      841 cctcaatttg taagaccact tgttaatcct atggcacaag ctgctggttt aagtaatagt
      901 gatgaactaa aagaatcgga tgaagaagcc ataaaggcat tatctgctgc atcagaaaac
      961 tatggaaatt tttatggtca aaacttattt gcaggtagta gtggagtatt acttatagca
     1021 tctactctga ctcaatttgg atacaatgta actggactta atatcgtaaa ggctagtact
     1081 attatggcag taatagcact tgtagtggct acaatccagt ttaccttata tgataaaaaa
     1141 ttaaacaaaa gtcataaaaa atagttttat ttaggaggaa taaaaatgca aaattttata
     1201 aacatatcat tagaaatctt ttacgcacta atgggatttt taatgattgt tatagcatat
     1261 aagtctttta ctaccataaa taacaataag aaatatggta catcattatt ttggatactt
     1321 atatcactgc cattcatatt tggtagattg attccagcca atataatagg aataatatta
     1381 atattatcta gtttattaac actttctaaa caagttgtat ttgcaaagta tgaagaacca
     1441 gatgaaaatt ttggaaaaga acaagcagac aaacttaaaa ataaaatatt tataccatca
     1501 ttaatattag catttgctgc agtagtagta gctatgagcc tttctaattt tgataattct
     1561 tctcaatttg caataggtgt gggttcaata attgcattaa tatcagccct tataataact
     1621 aaggcaaaac ctgctacatc agttcaagat ggctcaagat tacttcaaca aatgggacct
     1681 gctagtatgt taccacaact tttagttgca ttgggagctt tatttactca agccggagtc
     1741 ggtgaagtta tttcaaccat gatatctggt gtagtccctg ctgatagcag attatttggt
     1801 gttatagctt atgtattagg tatggttata tttaccatga taatgggaaa tgcatttgcc
     1861 gcattttcag tcataacggc aggaattggt attccttttg tattgtctca aggtggaaat
     1921 cctgctataa ttggagcatt agcattaaca gcaggatatt gtggaactct acttactcca
     1981 atggctgcta attttaatat agttccagca gccttgctag aatgtaaaaa tgattatatt
     2041 gttataaaat atcaagctcc agttgctttg gtattaatta tagcccacat attagtaatg
     2101 tactttttag gattttaata taattaggag gtaaaataat gaaaattttg ttaacaggat
     2161 ttgacccatt tggtggagaa ccaataaacc cagctcaaga agcagttgaa agggtcaata
     2221 ataatataaa cggtgcagaa atcataaaga taactatacc aacagttatg acaaaatctg
     2281 ttgaagctat agataaagct attcaagagc acaatccaga tatagtaata tcagttggac
     2341 aagctggagg tagatttgac attacacctg agagagttgc tataaatata gatgatttta
     2401 gaataaaaga taatgagggt aaccaggtta tagatactat tataaaagaa gatggtgaac
     2461 ctgcatattt ttcaaaattg cctgtaaaag ctatggtaaa acatatgaat gaaaataaaa
     2521 ttccagctag tgtttcaaac actgcaggaa catttgtttg caatcatgtg atgtatggaa
     2581 ttctttatat gatagacaag aaatatccta atataagggg tggatttata cacatcccat
     2641 atacaacttc acaagtaata gataaaaaaa atacaccatt catgtcttta gaagaaatag
     2701 taaaaggctt agaattagct atagaagctt gtattatata taaagaagat gtaaaagaaa
     2761 ttggtggtga aatttcataa taaatttttt agtattttta taaattaaaa atactaacta
     2821 ttaatttaaa tatagactgt gagtagatta ttcaaacaat tttactcaca gtctttttat
     2881 ggtatctaca gatacaaaat taaggtctat tcctctctcc ccactcacac attgcatcta
     2941 aaattggaat cagagatttt cccctttctg atagactata ttctacctta ggaggtattt
     3001 gaggatattc agtacgaata atcaagttat ctgcttctaa ttctttt
//
LOCUS       DAEPIN010000171         2981 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.174, whole genome shotgun sequence.
ACCESSION   DAEPIN010000171 DAEPIN010000000
VERSION     DAEPIN010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2981
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.174"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            424..2235
                     /locus_tag="KR250_003778"
     CDS             424..2235
                     /locus_tag="KR250_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HBH0069655.1"
                     /translation="MKKKYDFLIYSAIWLVIIILFIFSLYHSIEHIKIISHTGTIRSE
                     TQKVVKQELNNERNDDLIKRLDNILIKLRTGNGENGFQRCDNKEFQQKLNQMDSMWES
                     MKKEIIKVRNGASGDKLYKLSEEYSVLSNQIVFISEKHSNAKLYSFATALFIYLIFST
                     ISLLIWEYYNKKRFKRIFYTDNLTKIKNQVAFENRAIEILHNASNKEYVLLNIDIDNF
                     KYINDTHGYEYGDKVLIIVAAALSKTFNIKETCARIGSDNFVILAKYRDSLLEDIREM
                     LTNAIISELDMNVTQTISYCIGAYLVEIDNLGYKSINSIMDKANIAHKVSKTRGISST
                     VWYNENLLKQLQMENSIYNYMYKAIENEEFHMYLQPKFQISSLNVVSAEALVRWFSPE
                     LGFLSPDEFIPLFEKSGFIIELDFYMLKKACSFVRKTFMKKNQYTYPIAVNFSRVTIY
                     QNSFYQRFLDTVKEYEIPFKYIEIEVTESAFNEISQPVISILEELKKLGFLISMDDFG
                     SGYSSLSLLCSLSINGLKLDKSLLKETFNREKVYSIIQCIIEMSHRIGMSVVCEGIET
                     KKDLEFLNTVKCDVGQGFYFSKPIEEKEFFNKYVTKK"
     gene            complement(2323..2904)
                     /locus_tag="KR250_003779"
     CDS             complement(2323..2904)
                     /locus_tag="KR250_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069656.1"
                     /translation="MLYLLKINQFEKSYDIGIFKNEQSIYKFIAKIPFVKKDNSNFNY
                     TNYYMEFEDIPEYYEVNFNNYLYIITRFSFIPDGTEIFFTWEEIHCWDEKVATEKTFI
                     NGETLVDSYNFPNNEVKDYIQKRESLYIETKKYYEEKGLKIERHALGSEDGEYVELTN
                     DSILYLLDIEAVEIREQSKNIEEFLYKYKKRFK"
BASE COUNT         1132 a          307 c          411 g         1131 t
ORIGIN      
        1 caaaaaaaag gaatatattt acataatatg ataaaataaa ttcaaagagt taaattgtag
       61 gtaaatagta ttttaattat ttattaaaat atagattatg tatatgccaa atttaaaaag
      121 agacataaag acattataca aatatattat tggggtgata ttttataaga aaagaagtga
      181 tgcagtaaat taattgagca atttaaaaat gatttaatga tagtgttgat gaatgtttaa
      241 aaagaaattt tagaaaaacg tatattgctg tttgtgttgg aggtgatgaa tttgtataat
      301 ttataggata taaatgatta aactatttaa gataaataca tatcactgta aaatattgat
      361 aatcattttt tataagagat tgattatttg caaattatat atattacaaa tgaggtaaca
      421 ataatgaaga aaaaatatga ttttttaatt tattctgcta tttggctagt tattattatt
      481 ctttttatat tttctctata tcacagtata gagcatatta aaattataag tcacactggt
      541 actattcgca gtgaaactca aaaggttgtt aagcaagaat tgaataatga aagaaatgat
      601 gacttgataa aacgactgga taatattctg ataaagctaa gaacaggtaa tggagaaaat
      661 ggatttcaaa gatgtgataa taaagaattt caacaaaaac taaatcaaat ggattctatg
      721 tgggaatcta tgaagaaaga aattataaaa gttagaaatg gtgcttcagg tgataaatta
      781 tataaattga gtgaagagta ttctgtgttg tctaatcaaa ttgtttttat atctgaaaaa
      841 cactctaatg caaaattgta ttcttttgct actgcactat ttatttatct tatattctca
      901 acaataagtc ttttaatttg ggaatattat aataaaaaaa ggtttaaaag aatattttat
      961 actgataatt taacaaaaat aaaaaatcag gttgcatttg aaaacagagc gattgaaatt
     1021 ttgcacaatg catctaacaa agagtatgta ctgctaaata ttgatattga caattttaaa
     1081 tatattaatg atacacatgg atatgaatat ggagataaag ttctaataat agttgcagct
     1141 gcactttcta aaacttttaa tatcaaagaa acttgtgcaa gaattggttc agataatttt
     1201 gtaatacttg caaaatatag agattctctt ttagaggata ttcgggaaat gctaactaat
     1261 gctataatct ctgaattaga tatgaatgta actcaaacta tatcttattg tataggagcg
     1321 tacttagttg aaattgataa tttaggttat aaatccatca attctataat ggataaggca
     1381 aatattgcac acaaggtaag taaaacaaga ggaatttcct ccacagtatg gtataatgaa
     1441 aatctgttaa aacagttaca aatggaaaat agtatttata attatatgta taaagcgata
     1501 gaaaatgaag aatttcatat gtatttgcaa cctaaatttc aaatatcttc tttaaatgtt
     1561 gtaagcgcag aagctcttgt aagatggttt tctccagaac ttggtttttt atcaccagat
     1621 gaatttattc cattgtttga aaaaagtgga tttattattg aattggactt ttatatgtta
     1681 aaaaaagcgt gttcatttgt aagaaaaacg tttatgaaaa aaaatcaata tacatatccg
     1741 attgcagtta atttttcaag ggttactatt taccaaaata gtttttatca aaggttttta
     1801 gatactgtta aagaatatga aattcctttt aagtatatag agatagaggt aactgaaagt
     1861 gcttttaatg aaatatctca accagttatt tcaattttag aggaacttaa aaagttagga
     1921 tttttaattt caatggatga ttttggttca ggatactctt cattaagtct tttatgttct
     1981 ctatctatta atggattgaa gttagataaa agcttactta aagaaacctt taaccgggag
     2041 aaagtatata gtattattca atgtattatt gaaatgtcac atcgtatagg catgtctgtt
     2101 gtatgtgaag gtattgaaac taaaaaagac ttggaatttt taaatacagt taaatgtgat
     2161 gtaggacaag ggttttattt ttctaaaccg atagaagaaa aagaattttt taacaaatat
     2221 gtaactaaaa aataatataa ttatttttat cagtctatac attgagtcat tttatatctc
     2281 gttaaatgac tcaatgtact gttatgtatt ttaaaatata aattatttaa aacgtttttt
     2341 atacttatat agaaattctt ctatattttt tgattgttct cttatttcaa ctgcttctat
     2401 atctagtaaa taaagtatag aatcatttgt aagttcaaca tattcaccat cttctgaacc
     2461 gagtgcatgt ctctcaattt ttaatccttt ttcttcataa tatttttttg tttctatata
     2521 taatgattct cgcttttgta tgtagtcttt cacttcatta tttggaaagt tatatgaatc
     2581 cactaatgtt tcgccattta taaaagtttt ctcggtagca actttttcat cccaacaatg
     2641 gatttcttcc caagtaaaaa atatctcagt tccatctgga atgaatgaaa acctggtaat
     2701 aatatataaa taattattga aatttacttc ataatattca ggaatatctt caaattccat
     2761 atagtaattt gtataattaa aattactatt atcttttttt acaaagggta tttttgcaat
     2821 aaatttatat attgattgtt catttttaaa aattcctata tcataacttt tttcaaattg
     2881 attaattttt aataaataca acattctttc tccttttatt caaaatataa tttttataaa
     2941 tttactaatc tttagctttt ttaattatat atttacatat g
//
LOCUS       DAEPIN010000172         2933 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.175, whole genome shotgun sequence.
ACCESSION   DAEPIN010000172 DAEPIN010000000
VERSION     DAEPIN010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2933
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.175"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            complement(427..978)
                     /locus_tag="KR250_003780"
     CDS             complement(427..978)
                     /locus_tag="KR250_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0069657.1"
                     /translation="MSINTVIAKNLNRLRNERNLSLGQLAELSGVSKVMLSQIEKGDS
                     NPTVNTIWKIASGLNVPYTAILEQPQNETFIVSKTDIDVQVSENKDYRLYCYYPNTPT
                     RNFELFQMELEEGHSYTSVGHSEKSQEYIMIIEGQLKLEVNDSIYQLRENDSICFSAE
                     SIHTYHNQGEKTLKAVIINYYPV"
     gene            1169..2131
                     /locus_tag="KR250_003781"
     CDS             1169..2131
                     /locus_tag="KR250_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HBH0069658.1"
                     /translation="MRVLNNVTEAIGNTHMLHLSKIADEFGVQGNIYAKMEYLNPGFS
                     KKDRVALQMITEAENAGLLVPGQAVVELTSGNTGTGLAIACACKGYKFIACMSKGNSM
                     ERARMMKALGAEVVLVDQMPNSVHGQVSGEDLALVEIEAQKIAKERNAFRADQFNLEA
                     CNHAHEKYTAEEMWEQLDGNIDVFVDFLGSGSTFAGCSKTLKKHNSSIKCYVVEPETA
                     AIYSGKEITDQGHKIQGGGYSMDLPLVDKKLIDGYIQVTDEEATDVARKLAKLEGVFA
                     GFSSGANVCAAIKLLKSSEKGKNIVLTLNDSGLKYLSTDLYEEL"
     gene            2426..>2933
                     /locus_tag="KR250_003782"
     CDS             2426..>2933
                     /locus_tag="KR250_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HBH0069659.1"
                     /translation="MQKRILRNFIFTGKNILSKLVFIIKYNFSTLILFELFHKGLALF
                     FIWPSIRYIFDILIKSLHIVYLNMDNFIKVLTNPMSIVLIILSVIILAFYAFFEFTSV
                     IICFNKSIKYEKIGLFELFKISFENSIKLLYPKNILLFIFVILIIPLVNTVLISGFIG
                     KIQIPEYIL"
BASE COUNT         1129 a          307 c          458 g         1039 t
ORIGIN      
        1 atataaaata atttatttaa ctacttaaaa ataaactaaa agggataata aaatagcttt
       61 tagtttatgg taatagaggt atttaaaaag agtgcataag cactccaatt gtgagaaata
      121 catacacaaa tcattattac aacaatgaaa aatgtacata taagagacgt tttgtatttg
      181 ttttaagtga taatatttta atactaacta aataattatc acaatggtaa aaaagaaaaa
      241 ggagtatttt gtactccatt tgtcaaaaat ataaaacttt tatataagct tattataaca
      301 taattgataa aagtgtaaag atatataatg aatttataaa gaaaaataaa ataaaaaaga
      361 taaatcctaa tcaaaggatt tatctttttg tatagaaaat aattattcta ttattaaatg
      421 gcatggctaa actggataat aatttattat tactgctttc aatgtttttt ctccttgatt
      481 atggtatgta tgaattgatt cagctgaaaa acaaatagag tcattttctc taagttgata
      541 aatagagtca tttacttcta attttaattg accttctata atcataatat attcctgtga
      601 tttttcagaa tgaccaacag atgtataact atgaccttct tctaattcca tttgaaataa
      661 ttcgaagttt ctagttggtg tattgggata ataacaataa agacgataat ctttattttc
      721 tgaaacttgt acatctatat cagtttttga tacaataaaa gtttcatttt gaggttgttc
      781 cagtattgca gtatatggaa catttagtcc acttgcaatt ttccaaattg tgtttactgt
      841 tgggtttgag tctccctttt caatttgaga aagcattact ttacttacgc cagataattc
      901 agcaagttga cctagactta agttacgttc attacgtaat ctatttaaat ttttagcaat
      961 aacagtatta atactcatag tagctcctta aaatgtaaat tattgacaaa tataatttac
     1021 gaatgtataa tttatttaac attatttata atttacaaat gtataatata atgaacgtta
     1081 aatacactgt acatccgtaa tataaattat actgcataaa gagttgtttt tcaacggatg
     1141 atagttgaga gattaagggg gaattgtaat gcgtgtactt aataatgtta cagaggctat
     1201 aggcaatact catatgttac atttatccaa aatagcagat gaatttggag ttcaaggtaa
     1261 tatttatgca aagatggaat acttaaatcc gggatttagt aaaaaggacc gtgtagcatt
     1321 acaaatgatt accgaagcag aaaatgcagg tttattagtt ccaggtcaag cagttgtgga
     1381 attaacaagt ggtaatactg gaacaggact agcaatagct tgtgcatgta aaggatataa
     1441 atttatagcg tgtatgtcaa aaggaaattc tatggaaaga gcaagaatga tgaaagcttt
     1501 aggtgctgaa gtagttcttg tagaccaaat gcctaattca gttcatggac aggtatctgg
     1561 tgaagatttg gcacttgtag aaattgaggc tcaaaaaata gcaaaagaaa gaaatgcatt
     1621 tagagcagac caatttaatt tagaagcttg taatcatgca catgaaaagt atacagcaga
     1681 agaaatgtgg gaacaattgg atggaaatat tgatgtattt gtagattttc ttggaagtgg
     1741 tagtactttt gcaggatgtt caaaaacact taaaaaacat aattcttcta tcaaatgtta
     1801 tgttgttgaa cctgaaacag cagcaattta ttcaggtaaa gaaataactg accaagggca
     1861 taaaatacaa ggtggaggat attctatgga tttgccatta gtagacaaga aacttattga
     1921 tggttatatt caagttacag acgaggaggc aactgatgta gcaagaaaat tagctaaatt
     1981 agaaggtgta tttgcaggat tttcttcagg ggcaaatgta tgtgcagcaa ttaagttgtt
     2041 aaagagcagt gaaaaaggaa aaaatatagt tttaactctt aatgatagtg gtttaaaata
     2101 tttaagtact gatttatatg aagaattgta ataatagcac aatagttatg caatgttttt
     2161 taaattaagt atatagattt aaattataaa aagtatctca atgtaaattt tgaaagttat
     2221 aaaggagata cttttttatt ttgtaaatat ggagtatcct acttataagt attaagctat
     2281 atagatattg atgattagct atgcaataaa aaagagtatg tgtttttgca tttatacatc
     2341 ataacttgga ataatacata ttaatgttct aaatgtattt aaaatatatg ataaaattag
     2401 aacaaataga tacaaggaga taaaaatgca aaagagaatt ttaagaaatt ttatatttac
     2461 gggtaaaaat attttaagta aacttgtttt tataataaaa tataatttta gtacattaat
     2521 tttatttgaa ttatttcata aaggattagc cttatttttt atatggccat ctattagata
     2581 tatatttgat attttaataa aaagtttaca tatagtctac ctaaatatgg ataattttat
     2641 aaaagttttg accaatccca tgtcaatagt tctgataata ctatcagtta taatactagc
     2701 tttttatgca ttttttgaat ttacctctgt aattatttgt tttaataaat cgataaaata
     2761 tgaaaaaata gggttatttg aactctttaa aatatcattt gagaactcta taaaattgtt
     2821 atatcctaaa aatatactat tatttatatt tgtgatatta ataattccat tagtaaatac
     2881 agttttaatt tcagggttta taggaaaaat acaaatacca gagtacatat tag
//
LOCUS       DAEPIN010000173         2841 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.176, whole genome shotgun sequence.
ACCESSION   DAEPIN010000173 DAEPIN010000000
VERSION     DAEPIN010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2841
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.176"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            133..618
                     /locus_tag="KR250_003783"
     CDS             133..618
                     /locus_tag="KR250_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HBH0069660.1"
                     /translation="MISELMLKNFRKSSINFKTKIKLNLREKPDINSLKLKSIPEGKI
                     VKLKCVDGIWAEVESNYDKGWLLYKYLERLSNAKNNSNDTIKNRKKNYIGNIRTNGLS
                     LELRNDRTLESKVITTIPDGFKVEVCYSVGKWARVNINKNEKRYSGYVYNQYIEEHLS
                     L"
     gene            974..1372
                     /locus_tag="KR250_003784"
     CDS             974..1372
                     /locus_tag="KR250_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069661.1"
                     /translation="MNKKKKLIGIAISFLLIISIINPLQVEAEAIIESSVYISSIKEN
                     YTYDNTKFKTIKSKSRHKSRKYNKSKVKLKKRSIRTKKYGYQTINKYKRQNARRHNIL
                     TKLILFIIVIFLVIVLVVLIFIKRKKKKFY"
     misc_binding    1807..2070
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2221..2772
                     /locus_tag="KR250_003785"
     CDS             2221..2772
                     /locus_tag="KR250_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan transporter"
                     /protein_id="HBH0069662.1"
                     /translation="MKTNTKKLTLNAILLAMGLLIHQITPAIGLPMKPDVPLAMLFVI
                     LVLNRDDYKTCLIAGIVTGIFTALTSSFPGGQIPNVIDKTLTANIVFLLMNISYNMPF
                     IKKLAKKTQDTVVVAIIMPIGTLVSGTIFLLAAQAIVGLPGASFTALFLAVVLPAVLI
                     NLVAGIFLFKIVSLSIRRVSYQA"
BASE COUNT         1231 a          253 c          426 g          931 t
ORIGIN      
        1 tagggactaa aatctttatg taataaaggt catgtcagcc agttgccaaa aagataataa
       61 ttctttttgt taaaagtatt tttagagtta tatattttaa taacttaata tttggcaaga
      121 ggagggctta agatgatatc tgaattaatg ttaaaaaatt ttcgcaaatc tagcatcaat
      181 tttaagacta aaattaaatt gaatttaaga gaaaaaccag acataaatag tttgaaatta
      241 aaaagtatac cagaaggtaa aattgttaag ttaaagtgtg tagatggaat atgggcagaa
      301 gttgagagta attatgacaa aggttggtta ctttataaat atttagaaag attaagtaat
      361 gctaaaaaca atagtaatga tacaataaaa aatagaaaaa agaattacat aggaaatata
      421 agaacaaatg gattaagttt agaacttaga aatgatagaa cattagaatc taaagttata
      481 actacaatcc ctgatggttt taaagttgaa gtatgttata gcgttggaaa atgggcaaga
      541 gttaatatca ataaaaatga aaaacgatat tctggatatg tatataatca atacatagaa
      601 gagcatttaa gtttataata cgatactagc aagctttaaa tatattaata aatcgcaaaa
      661 aagaatccca ttaatgggat tcttttttgt agaaacatta taacagaact aaagagaata
      721 aattaactaa tttattttta ttataaagta attataaatg gtaaatttaa ttgaaagtag
      781 atttaactta tactttatac accaagtatc ataaattaat actactttga aaaatcttaa
      841 aaaaagtaaa agttatgata ataaaaataa tattaaagat aataagatat ataagaaact
      901 tgaaaaaatt tgtaattttt agttatatat atattataat gaactagtta aaaattttta
      961 tgagggggaa aatatgaata agaagaaaaa attgattggg atagctatat cttttctact
     1021 tataatcagc attataaatc cattacaagt agaagcagaa gctattattg agtctagtgt
     1081 atacatttct tcaataaaag agaattatac atatgataat actaaattta aaactattaa
     1141 atctaaaagt agacataaaa gtaggaaata taataaaagt aaagtaaagt tgaaaaaacg
     1201 aagtataaga actaaaaagt atggatatca aactataaat aaatataaaa gacaaaacgc
     1261 aagaagacat aatatcctaa caaaattaat attgttcata atagtcatat ttttggttat
     1321 agttttagta gtgctaatat ttattaaaag aaagaagaaa aaattttact aaaaataatt
     1381 aaaagaatat cattttttta atcattggta tctatttaat aatatgatgt aattaaaatg
     1441 gagtaatatt ctgtttcaaa ggaggcatat aaatacagga atgagtatta aatgatggtt
     1501 aattaagtaa atttattgat aaatgtactt aattaacttt gaagaattta attcctcatg
     1561 aatcaaaata taaaattaca tcaacagtta ttcacaaaat taatataaga agaattagag
     1621 ccaaagagca gactattaga ccattaaagt aattattcat agaattttgt atatgaaaag
     1681 aaatgtatag tataatttac tttttttata aaaaatagat tacaaaatat attttttata
     1741 aaaaggtatt gacatttttt agaaaaaaga atattattat tttaaaacaa aattcaatac
     1801 aataaattaa atcctatgac aaggaagagt agttaaattt aggttcagta tagagagctg
     1861 aggatggtgg aatcttagca agaaaaaatt tgatgaatgg cccttagagg aataaattga
     1921 aatcatagtt ttgagagtag actttatcgt cagtcgcacg ttatagcgaa taaggtatgt
     1981 aagtacctga taatgagatg tatgaaaatt gaattttata ttaattttta tataatttag
     2041 gtggcaacgc ggataatctc cgtcctatta gtttaggacg gagtttttta ttttactgaa
     2101 aatttaaaag atagaaggtg gtgagaaaga tgttatgaat ttcaaaagct aaagatgatg
     2161 atatagtagg ttggcaaaaa aataaattga aaattaaaaa aataggagga agtacaaaaa
     2221 atgaaaacaa atacaaaaaa attaacttta aatgcaatac ttttagcgat gggattatta
     2281 atacatcaaa tcacaccagc tataggatta ccaatgaagc cagatgtacc actagcaatg
     2341 ttatttgtaa tattggtttt aaatagagat gattataaaa cttgtttgat agcgggtata
     2401 gtaacaggaa tatttacagc acttacatca agttttccgg gaggtcaaat tcctaatgta
     2461 atagataaaa ctcttacagc aaatatagta tttttattaa tgaatattag ttataatatg
     2521 ccttttataa aaaaattagc taagaaaaca caagatactg tagtagttgc aataataatg
     2581 ccaataggaa ctcttgtaag tggaactatc tttttattag cagctcaagc tatagttggt
     2641 cttccagggg cttcatttac agctttattc ttagcagtag tattaccagc tgttttaata
     2701 aatctagttg caggaatatt tttattcaag attgttagtc tatcaataag aagagttagt
     2761 tatcaagctt aaacacgaaa taatatttta ctttataaag cattataata actaaataac
     2821 ataagaataa agtagattat a
//
LOCUS       DAEPIN010000174         2829 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.177, whole genome shotgun sequence.
ACCESSION   DAEPIN010000174 DAEPIN010000000
VERSION     DAEPIN010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2829
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.177"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            complement(<1..594)
                     /locus_tag="KR250_003786"
     CDS             complement(<1..594)
                     /locus_tag="KR250_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HBH0069663.1"
                     /translation="MINDIKNKFKNYKPYINGYKNMKRASVLIPIVEINNTHYILFEV
                     RSKNLKHQPSEISFPGGKIESGETPYEAVIRETCEELGTFSDNIEVISSLDLLITPVK
                     FIIHPYLGYIKNINNLNINKDEVDHTFLVPIKYLLENPPNTYSNTVKVFPDENLPYGS
                     IPNQKDYKFESGSYPILFYKYKDYVIWGMTAKILENFL"
     gene            complement(825..1738)
                     /locus_tag="KR250_003787"
                     /pseudo
     CDS             complement(825..1738)
                     /locus_tag="KR250_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007061260.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
     gene            1994..2350
                     /locus_tag="KR250_003788"
     CDS             1994..2350
                     /locus_tag="KR250_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0069664.1"
                     /translation="MDIDNNFEEFPNIEERQKDFNCSIGFTMTVIGSKWRAIILWHIL
                     KQEPIRYSQLKKEVVHISHKILSQELKYLERDGLIKRISYPTIPPKVEYLSTKRGKSL
                     ESILLELCNWGKQYMR"
     gene            complement(2465..2641)
                     /locus_tag="KR250_003789"
     CDS             complement(2465..2641)
                     /locus_tag="KR250_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069665.1"
                     /translation="MFMDKMDRCTHILTAYICSSRDYCNFIDTQLNDFILEYGENVVE
                     SCLHQVMVLVSRYN"
     regulatory      complement(2694..2783)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
BASE COUNT          992 a          370 c          361 g         1106 t
ORIGIN      
        1 taaaaagttt tctagtattt ttgctgtcat tccccaaatt acataatctt tatatttata
       61 aaacagaata ggataacttc cactttcaaa tttataatct ttttggtttg gaattgaacc
      121 atatggtaga ttttcgtctg gaaaaacttt tacagtatta ctataagtat ttggaggatt
      181 ctctaaaaga tattttatag gaaccaaaaa agtatggtca acttcatcct tgtttatatt
      241 taaattatta atatttttta tatatcctaa gtatggatgt atgataaact ttactggtgt
      301 tataaggagg tcaagacttg atattacttc tatattgtca ctaaaagttc caagttcctc
      361 acaagtttct ctaataacag cttcatatgg agtctcacct gattctattt taccacctgg
      421 aaaagaaatt tcactaggtt gatgcttcaa attttttgag cgaacttcaa ataatatata
      481 gtgtgtgtta tttatttcta ctattggaat taaaacagat gctctcttca tattcttgta
      541 tccatttata tatggtttgt aatttttgaa tttattttta atatcgttta tcatttttac
      601 ctcctaaaat aatattatta aaaattatat aattgagttt taacaataat atataattat
      661 gatttatcta attataagat atagtaacta tctatattat tatataatat aatgataaat
      721 atatattaac aaataataaa aaaattaatt tgattgtata taaattagtt tgttattaaa
      781 ttagtattaa aaagttcaaa aatttactta gaaggaatct ataattagat ttctatgttt
      841 gttgagaatg caattacagc ttttccaatt atttttactt caactggttt attatctaaa
      901 ataattactt ttactaacat cttgcctttg cgttttattg tttctccttg aactgcttca
      961 aatataaact ctgtgttggg tgtaggtatt actttataat gtacaaggta cgcacctaag
     1021 ggtccattag catttcctgt aacagggtct tctgaaatac caattgcagg agcaaacatt
     1081 cttccatgaa cttgtagagt ttctttatta tgtaatgtaa aaatataata tccattacat
     1141 tttatttctt gacttatttt cgataattca tataaatttg gagataaact atttaatgtt
     1201 gataattcct ttattccaat cataactttt gaatgtcctg tagatgatat ggcaatagga
     1261 caatctgtac gaatatcttc ctttaaaata ccaaatgcat taagtaatct tttttgatag
     1321 gtttgttgta acggtggaga tacttcaatt tttccttgtg tcataattat ttcataatca
     1381 ttatcttttt ttataatatc aactggtaat attccagcac tagttttttg aaatactgtt
     1441 ttgttattga attggttttc tactgctcta acataatggg cagcaatagt agcatgacca
     1501 catataggaa cctcattagt tagtgtaaaa aatctgatat gtacatcata attttcttga
     1561 tgatttttag ccaagttatt gaaaataaaa gcagtttctg aattatttaa ttctcttgct
     1621 attttttgca tttgtgattc agataagcca tcagcatttg taattacacc agctggatta
     1681 cctgtaaatt tttcttttgt aatgagtcaa cttgataaag acgaaatttt ttattcatat
     1741 tatataatcc tttctaaaca ttagattagt ttatctacat agaaaacttg agataaatta
     1801 ttatttatta ttatctgttt aaataaaatt aataaaataa catatgtcta tattaaagtt
     1861 taaacatata catctaaaaa agtagtatta tgtaaggctc tatactacaa tcaagattat
     1921 attacgaatt agtacgctaa acaagaatgc actttttagt tagatactta ctattttgaa
     1981 agagaggatt gagatggata tagataataa ttttgaagaa tttccaaata ttgaagaaag
     2041 acaaaaagat tttaattgtt caattggttt taccatgact gtgataggaa gtaaatggag
     2101 ggcaattatt ttatggcata ttttaaaaca agagccaata cgttacagtc aattaaaaaa
     2161 agaagttgtt catataagtc ataagatact gtcacaagaa ttaaagtatc ttgaaagaga
     2221 tggacttatt aagaggattt cttatccgac tattcctcca aaagttgaat atttgtctac
     2281 taaacgtggt aaatcattag aatctatatt attagaatta tgtaattggg gtaaacagta
     2341 tatgagataa actcaactgt ttactttatg aaaaatatat aatgaagctt attttaatta
     2401 gttctttaat gaattataaa acgtagataa aactaaaaag ggttttctag aaaataaaat
     2461 ttctttaatt atatctgctt accaatacca tcacttgatg caaacaagat tctaccacat
     2521 tctctccata ttctagtata aaatcattta attgtgtatc tataaaattg cagtaatcgc
     2581 gtgaactgca tatatatgca gttagtatat gtgtacatct atccatttta tccataaaca
     2641 tatatcatat atccccctaa aaaacattca taaaaacaaa aagaaaatat ctctttggca
     2701 actggctgac ataactcatt tcataagtct tagtccctat agctttgcgt cactaaattt
     2761 ctctagtttt gcctatttag ttttattcta ctattaccat aatataggaa aacgtttctt
     2821 ttttcaagg
//
LOCUS       DAEPIN010000175         2672 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.178, whole genome shotgun sequence.
ACCESSION   DAEPIN010000175 DAEPIN010000000
VERSION     DAEPIN010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2672
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.178"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            786..1187
                     /locus_tag="KR250_003790"
     CDS             786..1187
                     /locus_tag="KR250_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNHCP domain-containing protein"
                     /protein_id="HBH0069666.1"
                     /translation="MNYKNRKRAYYQENSCMDNFICKNCGCLVVPEGAGTQHRNHCPH
                     CLHSLHVDIIPGDRAADCDANMEPIGVWVRKNGEWAIIHRCTRCGHLSSNRIAADDNS
                     VKLMSIALKPLAQPPFPLERIIEKLEKEETE"
     gene            1184..2251
                     /gene="rsgA"
                     /locus_tag="KR250_003791"
     CDS             1184..2251
                     /gene="rsgA"
                     /locus_tag="KR250_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HBH0069667.1"
                     /translation="MNGMKQINLQDYGLNRNFITQSMEYHDLYLARIISQEKNFYQAV
                     CVNGVLFAEVSGKLRFEATIQSDFPTVGDFVMLDRCNNKNGNAIIHHVLHRKSLFVRK
                     AAGKVNEEQVVAANIDIVFLCMSLNKDFNIHRLERYLSLTWESGAIPVVVLTKSDLCD
                     NVHRKLQVVNSIALGVDVVVTSSIEPDGYKQISKYIKPGCTVALLGSSGVGKSSLINC
                     LMGGEFLNTKEIRIDDDKGRHATTRRELFLISGGGIVIDTPGMRELGMWDVSEGLDKS
                     FADIEAYFGKCHFRNCTHTSEPGCAIWEAIAHQELSPKRWESYRRLKTEAVFADNKSE
                     YLAKKEKKFKNISKINKKIRR"
BASE COUNT          949 a          309 c          489 g          925 t
ORIGIN      
        1 tacaataata caatagaatt gcttattatt caatatactt atttgaaaat taaataaatt
       61 tacaaatatg aacgttattt tatatcaaaa aaaatagact atgcatgtag aatatatgga
      121 aaaacttgat tgtaggtttt gtatgaaaag acagttgtta ttaatttaag tgagataaat
      181 tgagcaacag gcataaaatg agaaaacaaa taatagcagt taatttttat tattaatact
      241 aggtgaacat taaatttcaa aataccacta gattttatag aataagaaaa tgagacaaac
      301 ataaatatat gaatatatta aattatactt gaacatactt atattttatg ataaaatttc
      361 aataataatt attagtgaag gagtgtcttg cctgatgtca gagttatata taatggtaac
      421 caacaaataa atagttggag acgcatatgg gttctgttta atcagagttt ttaagtgtat
      481 ttttgataca tttgattgtt gtgtcttact ctgcctttat agataactaa cattagacaa
      541 caaataataa tattacatat tactttgtta aattgtggtg tagtgggctt taatctgcta
      601 cacttttatt aattttacac agtattattt tgaatataat tatgttgaaa tagaagatac
      661 taaattttct agtccacagg ggttttctgt ttttgtgtag gtaaatatgt ataccacata
      721 aaaaggcaga tataaatacc tgtggacttt ttttgtagaa aatacacgat acggaggaaa
      781 taaaaatgaa ttataaaaat agaaaacgag cttattatca ggaaaattcc tgtatggata
      841 actttatttg caaaaattgt ggttgccttg tagttcctga aggagctggt acacaacata
      901 gaaatcattg tccacattgt ttacatagtc ttcatgtaga tatcattcct ggtgaccgtg
      961 ctgcagattg tgatgctaat atggaaccta ttggagtgtg ggtgagaaaa aatggtgagt
     1021 gggcaattat tcatcgttgt acacgctgtg gacatcttag ttctaatcgt attgcagctg
     1081 atgataattc agtgaaatta atgtcaattg cattaaaacc actggcacag ccccctttcc
     1141 cactagaaag aatcattgaa aagttggaaa aggaggaaac agaatgaatg gtatgaaaca
     1201 aatcaattta caagactatg gtctaaatag aaattttatt actcaaagta tggaatatca
     1261 tgatttatac cttgctcgga ttatctcgca ggaaaagaac ttttatcaag ctgtatgtgt
     1321 aaatggtgta ctgtttgcag aggtttcagg aaagctgcgt tttgaggcta ctatacaatc
     1381 tgattttccc acagttggtg atttcgttat gttggatcgt tgcaataata aaaatggtaa
     1441 tgctattatt catcatgtgc ttcatagaaa aagcttgttt gtgcgtaagg ctgctggaaa
     1501 ggtaaatgag gagcaggtgg ttgcagctaa tattgacatt gtttttttgt gcatgtcact
     1561 gaataaggat tttaacattc atagattgga gaggtatctg tctttaacat gggaaagtgg
     1621 agctatccca gtagtagtac ttactaaatc agacttatgt gacaatgtgc atcgtaaatt
     1681 acaagtagtt aattctatag ccttgggtgt agatgtagtt gttacctcat ctatagagcc
     1741 agatggttat aaacagattt ctaagtatat taaaccaggc tgtactgttg ccttacttgg
     1801 ttcttcagga gttggaaaat cttcattaat taattgtctg atgggtggag aatttttaaa
     1861 tacaaaggaa attcgcatag atgatgataa aggaagacat gccacgacta gacgagagct
     1921 atttttaatt tcaggtggtg gtattgttat tgacacacca ggtatgagag agttgggaat
     1981 gtgggatgtt tcagaaggtt tagataaaag ttttgcagat atagaagctt atttcggaaa
     2041 atgtcatttt cgtaattgta ctcacacctc agaaccaggc tgtgctatct gggaagctat
     2101 tgctcatcaa gaactttcgc ctaaaagatg ggaatcctat cgtaggctta aaactgaagc
     2161 agtatttgca gacaataaaa gcgaatactt agcaaaaaaa gaaaagaaat ttaaaaatat
     2221 ctcaaaaata aataagaaga ttagaagata agtgtagttt ttttgttcat tctatgagtc
     2281 tatatactat taatatatta aatttgccat caatttgtta ctttaatgtg attttgatgg
     2341 caaatttggt ttgtggtatt atttaaagca atttattaat ttttttaata aagtttgatt
     2401 ttttatatga tgatatatac atgtgataat gtatacatat ttgttacgat aggctccttt
     2461 ataaagaggt cttatgtaag ataagaaaag gcatatttat actaatacat aatagagtat
     2521 atgcttatgt atgatagaga tgtttcttaa gagcaaaact gcaatattgc taatttataa
     2581 agtaaaaata tatgaattat aaaatacaaa taatatattt ataaaaatta ttgttttaat
     2641 ttataataaa aattttttgt tgaaaataat gc
//
LOCUS       DAEPIN010000176         2586 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.181, whole genome shotgun sequence.
ACCESSION   DAEPIN010000176 DAEPIN010000000
VERSION     DAEPIN010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2586
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.181"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            complement(238..1305)
                     /locus_tag="KR250_003792"
     CDS             complement(238..1305)
                     /locus_tag="KR250_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HBH0069668.1"
                     /translation="MSNIKIIASNKCWIEDIARKQLEDISKLNGILRVVGLPDLHAGK
                     IPVGLAVETKNIIYPHIIGNDIGCGMTLFKTGVLKKKFKKDKWIKSLSKIKDLSDIEI
                     KNTYKEECPILNLGTIGSGNHFVEIQCISEIYNKEQFEQLKFSSDDIMMLVHCGSRNY
                     GEDILKKFYDKDGLEVESEKAIDYIKKHDNALMWAERNREAISKKIMQSIGTSGDVET
                     IFSINHNFIEKREDKFIHRKGAVSSERGAVIIPGSRGSLSYIVMPTENTEISLYSLSH
                     GAGRKWSRSVCKSRLKSKYNKDTIKQTKFKSQIICNDVNLLFQEAPEAYKNIEQIIES
                     LIEYELIQVVATMKPLITYKG"
     gene            complement(1551..2546)
                     /locus_tag="KR250_003793"
     CDS             complement(1551..2546)
                     /locus_tag="KR250_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HBH0069669.1"
                     /translation="MYEEVTWLNFIGNRYEVVNSDDVLEINKIYKARDVFYRKNVLIK
                     VIKHNNYICEDFVSNLIDESTALDEINSPYILKIIDVGIHCTEETTLYYVVSEDFNGI
                     GLDELILGNYLHLEAIVNIMIQVLKALEMIHRNHSYHGSLKSSSIIVDTEYNIKICDF
                     GITKANNGVNTRSYGNRRYLCPHQLCINYTDKESDFFATGLILFESIFKKLPFGESNS
                     EEKMLQSIDKGLDWNSIKAINGNTELINVIKRLLGRNNKYTRANDIIIDLSKVMYEKA
                     YIEVEDEKEEEQKQDKVIQVKKEYKSKMRKKLHKKLVVAGLAIVMISMIIISSII"
BASE COUNT          836 a          445 c          231 g         1074 t
ORIGIN      
        1 atagtgaaaa taagcttata ctttcattta tatttgggaa tcacttatat tgtcatcaat
       61 tcatgtatgt gcattttcca atacagtcta actttgtaat cattatatat caaagaattt
      121 agctctataa attatcgtct ctacaaaata aaaaaatagc taataataaa acacttttaa
      181 atgttccata aaattagcta tttataatat aattttaatc ttttttaact ttaaaaatta
      241 tcctttataa gtaatcaatg gcttcatagt tgccactact tgaataagtt catattctat
      301 caaactttca ataatctgct ctatattctt atatgcctct ggagcttctt gaaataataa
      361 atttacatca ttacatataa tctgactttt aaatttagtt tgttttatag tatctttatt
      421 atacttactt ttaaggcgag atttacaaac tgaacgagac cattttctac ctgctccatg
      481 tgatagagaa tataaagata tctctgtatt ttcagtaggc ataactatat atgatagact
      541 gcctcttgaa cctggtataa tcacagctcc tctttcactt gaaacagcac cttttctatg
      601 tataaattta tcttcccttt tctctataaa attatgattt atagagaata ttgtttcaac
      661 atctccagat gtacctattg actgcataat ttttttactg atagcttcac gattacgctc
      721 tgcccacatt aaagcattat catgtttttt aatataatct atagctttct cactttcaac
      781 ttcaagtcca tctttatcat aaaatttttt taaaatatct tctccataat ttcttgaccc
      841 acagtgaact aacatcataa tatcatcaga tgaaaatttt aattgttcaa attgttcttt
      901 attataaatt tcacttatac attgaatttc tacaaaatga tttccactcc ctatagttcc
      961 taaatttaaa attggacatt cttctttata tgtgtttttt atttctatat cggacaaatc
     1021 tttaatctta ctaagtgact taatccattt gtctttttta aatttctttt ttagaacccc
     1081 agttttaaat aaagtcattc cgcatcctat atcatttcca attatatgtg gataaataat
     1141 atttttagtc tcaactgcta aacctacagg tattttacct gcatgtaagt caggtaatcc
     1201 tactactctt aaaattccat ttaactttga aatatcttca agttgtttcc ttgctatatc
     1261 ttcaatccaa catttattac ttgctattat ttttatattg ctcataattt gctccttttt
     1321 aatattctcc tgcctttaaa aagtagcaaa aagctgtgac ctttagccac agctttttta
     1381 ttctaaactg taaaagcagg tggctatttg ttaaaatact cctagacgtt taatacctca
     1441 ttattttttg ttaacataat aaccacttcc tttcataatt taagactaca ctaaaatttc
     1501 tatgtatatc ctttatagac attgatttta aaatacttaa tatacaattt ttatattatg
     1561 gaactaataa taatcattga aatcatgact atagctagac cggcaacaac aagtttttta
     1621 tgtaattttt ttctcatttt acttttatat tcttttttaa cttgtataac tttgtcttgt
     1681 ttctgctctt cttctttctc atcttcaact tctatatatg ctttttcata cattacttta
     1741 cttaaatcaa taataatatc attggctcta gtatacttat tatttcttcc aagaagcctt
     1801 tttataacat ttataagttc agtatttcca ttaattgctt ttatactatt ccaatctaat
     1861 cccttatcaa tagattgtag cattttttct tctgaatttg attctccaaa aggcaatttt
     1921 ttaaatatag attcaaatag aataagacct gttgcaaaaa aatcactttc tttatcagta
     1981 taatttatac atagttgatg tgggcataaa tatctcctgt ttccatagct tcttgtatta
     2041 accccattat ttgcttttgt aattccaaaa tcacaaattt ttatattgta ttctgtatct
     2101 actattatac tactcgattt taaactacca tgatatgaat gattcctatg tatcatttct
     2161 aaagctttta aaacttgtat cattatgttt acaattgctt ctaaatgaag ataattacct
     2221 agaattaatt catctagtcc gataccatta aagtcttcac ttacaacata atataatgtt
     2281 gtttcttctg tacagtgaat cccaacatct attattttta gtatataagg agagtttatc
     2341 tcatctaaag cagtactttc atcaattaaa tttgaaacaa aatcttcaca gatataatta
     2401 ttgtgcttta taacttttat taacacattt ttcctgtaaa aaacatctct agctttatag
     2461 attttgttaa tttctaaaac gtcatcagaa tttactacct catatctatt gcctataaaa
     2521 ttcaaccatg tcacctcctc atacatttta tataactaaa tctatttaca attataccaa
     2581 tatata
//
LOCUS       DAEPIN010000177         2560 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.182, whole genome shotgun sequence.
ACCESSION   DAEPIN010000177 DAEPIN010000000
VERSION     DAEPIN010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2560
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.182"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            636..2432
                     /locus_tag="KR250_003794"
     CDS             636..2432
                     /locus_tag="KR250_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002594337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HBH0069670.1"
                     /translation="MKPTMEILTKLQENSKKNHDEVFTRLYRYLLRPDIYYVAYQHLY
                     SNKGAGTKGVTDDTADGFSEIYIENIIEALKNEMYQPKPVRRTYIKKSNGKMRPLGLP
                     VFTDKLIQEAIRMILEAIYEPIFSDYSHGFRPARSCHTALAQIKKEFTGARWFIEGDI
                     KGCFDNINHAVLVEIINQKIKDARFLKLIRSFLKAGYMEDWKYHKTYSGCPQGGIISP
                     ILANIYLNELDRHVMKIKKEFDVATKARYTPEYTKLVGLRQRLHNKIKNSNGIEREKL
                     IEEYKSATAQMLKLPAKQCDDKKIKYVRYADDFLIAVNGNRQDCEKIKQELTEFISTT
                     LKMELSQEKTLITHSNTPARFLGYDVRVRRDQQIKPKGKFKTRSMNNKVELSIPFKDK
                     IEKFLFSNGIVKQRSDNGKLEPIHRPQLLNRTDLEIVTIYNAELRGICNYYGLASNFN
                     KLIYFNYLMEYSCLKTLAGKHRSKVSKIRAMYKDGTGKWAIPYETKTVIKKMYFANYA
                     DCKGKKFTDIVPQTAKNYSHDVTTLESRLKAKICEVCGCTESDRYEIHHVNKVKNLKG
                     KSEWEKIMIAKRRKTIVVCHKCHMAIHHGEKK"
BASE COUNT          977 a          439 c          533 g          611 t
ORIGIN      
        1 ttagaagtag attaaagaaa gggggacaga aaaaaaaggc actaccccgt aagataacac
       61 gggaaaccac ctgcctaacc gaaaggtgaa aactgtacgg gaaaatagca cggcaggaaa
      121 gcagaaagtt gttcaaagac aatagagcac gactgaactg caaggttaag tgaatatgag
      181 gataaaacta gatttgttga atgtgagttc caaacctctg ttacgtgtgg acacaggaaa
      241 cttgtctgaa acctgtggta tgagtaacgc tgaaaataaa tgcctttcag tgggttatca
      301 attaactcat acggtacgct ggagaaccag tagtaacgga acgaaagcat atccgacaat
      361 tcacaccagc ctactacttt cgtcaactgg agattaccta aaccagaatg cctacaatgg
      421 ctatgtgtaa tgctcttgta gtgataaatc acaaggctat gctaagtgag atgacgctga
      481 aaatctggca aggtaacgga gctttcatag tagtctgaga accctgaaaa gacagggaaa
      541 gccgtaaaat cggatacggg aaaaccgttc acatggcgaa ggaaagcagt cttagcgaat
      601 taaaattaga aattgattag ggaggaaaac ctcaaatgaa accaacaatg gagattttaa
      661 ccaaattaca ggaaaattca aaaaagaacc atgatgaagt atttacaagg ctctatcgat
      721 atttactccg tccagatata tactatgttg catatcaaca cctctactcc aacaaaggag
      781 caggaacaaa gggtgtaaca gacgacacag cggacggttt tagcgaaata tatattgaaa
      841 atatcattga agccttaaaa aatgaaatgt atcaaccaaa accagtaaga cgtacataca
      901 tcaaaaaatc aaatggcaaa atgcgtccct taggactgcc tgtctttact gataagctga
      961 tacaggaagc aatacgcatg atattagaag caatctatga gcctattttt tcagactact
     1021 ctcacggttt ccgaccagca agaagttgtc atactgctct tgctcaaata aaaaaagaat
     1081 ttacaggagc acgctggttt atagagggcg acataaaagg gtgctttgat aacatcaatc
     1141 atgcggtact tgtggaaatt atcaatcaga aaattaaaga tgcacgtttt ctgaaactca
     1201 ttcgttcatt cttaaaggca ggttatatgg aagattggaa ataccacaaa acatatagcg
     1261 gttgccctca aggtggcatc atttcgccta ttcttgcgaa tatctacctt aatgaattag
     1321 accgtcacgt aatgaagata aagaaagagt ttgatgtggc aacaaaggca cgttatacac
     1381 cagaatatac aaaactggta ggactacgcc aaagattaca caacaaaatt aaaaatagca
     1441 acggtataga aagagaaaag ctgattgaag aatataaatc tgcaacggct caaatgctta
     1501 aactaccagc gaagcaatgt gacgacaaga aaataaaata tgtgcgttac gctgatgatt
     1561 tcctaatagc tgtgaatggc aatcgacagg attgtgagaa gattaagcaa gaacttacag
     1621 aatttataag taccacatta aaaatggaac ttagtcaaga aaaaactctg attacacaca
     1681 gtaatactcc tgctcgtttt ctcggatatg atgtcagagt acgccgtgac cagcagatta
     1741 aaccaaaagg aaaattcaaa acccgttcta tgaataacaa ggtggaattg agtattccat
     1801 tcaaagacaa gatagagaaa tttttatttt caaatggaat tgtaaagcag aggtcagaca
     1861 atgggaaatt agaacctata caccgaccac aactgcttaa taggacagat ttggaaatag
     1921 tgactattta taatgcagaa ttaagaggta tctgtaatta ttatgggttg gcaagcaatt
     1981 ttaacaagct aatctatttc aattatctta tggaatatag ctgtttgaaa acacttgcag
     2041 gaaagcaccg ttccaaagtt tctaaaatta gagctatgta taaagacggt acaggcaaat
     2101 gggctattcc ttatgaaact aaaacagtaa taaagaaaat gtattttgcg aattatgctg
     2161 actgcaaagg aaagaagttt actgatattg ttccacaaac agctaaaaat tattcccatg
     2221 acgtaacaac attagaaagc cgattaaagg cgaagatatg tgaagtatgt gggtgtacgg
     2281 aaagtgacag atacgaaatt catcatgtaa acaaggttaa aaaccttaaa ggaaaatcag
     2341 aatgggaaaa gataatgata gccaaaagac ggaaaacaat cgtggtatgc cacaagtgtc
     2401 acatggctat acatcatggc gaaaagaaat aacctgtaac ggagcaaaga tggagagccg
     2461 tgtgcattga gaaatgcaag cacggttcgg ggagaggact gcacaaacct accctagaaa
     2521 taaggaaagg cggtgctttc ctactctact atgtgggaaa
//
LOCUS       DAEPIN010000178         2473 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.183, whole genome shotgun sequence.
ACCESSION   DAEPIN010000178 DAEPIN010000000
VERSION     DAEPIN010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2473
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.183"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            <1..1135
                     /locus_tag="KR250_003795"
     CDS             <1..1135
                     /locus_tag="KR250_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HBH0069671.1"
                     /translation="VVAIIGYAIYHSGIIFIGIWTKYCTDMEFLKNVFINRCIAFEEY
                     FRLILSIFIFILDIGFISATYYQYKGVNISKNNIKKEKKSISKIVFNLVLILLISYIE
                     SVAFSSHINGMFNTSFSFYTTAIAHRAGAEMAPENTLAAIEEASHAKADYAEIDVQET
                     KDGELIVLHDSNFKRTTGVDKNVWDVNYNEVKTYDAGSFYYYGKGYYDEKFVGEKIPT
                     LKEMIKYSKGKVKLLIEIKLNGHEKGDVVKKVVQLIKENNFENQCIVASMDKTILQKV
                     KKLDENLLTCYFMAIAYGDFSKLNYVDIYGIESTFVNKTVVEKIHKMNKKIFVWTVNS
                     DSLIEKMLDLNVDSIITDNPYLIESAIYWKKNGFISQVSDYLF"
     gene            1457..2041
                     /locus_tag="KR250_003796"
     CDS             1457..2041
                     /locus_tag="KR250_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HBH0069672.1"
                     /translation="MTEREKMLAGELYDCGDIELLTQWHKAKDLVRDYNQTDSLDLEK
                     KEKILNELLGGKGKNLWITAPYYVDYGNNIYFGNNCEVNMNCTFLDDNKIIIGDNALI
                     APNVQIYTAFHPTNAGDRFGNAKEDGSFEFCKTQTAPVTIGDNVWIGGGAIIMPGVTI
                     GDNVVIGAGSIVTKDIPSNMIAYGNPCRIIRENK"
BASE COUNT          961 a          229 c          397 g          886 t
ORIGIN      
        1 agtagtagct attattggat atgcgattta tcatagtgga attatattta taggcatatg
       61 gactaaatat tgtactgata tggaattttt gaagaatgta tttataaata gatgtattgc
      121 atttgaagaa tattttagat tgattttatc aatattcatt tttatattgg acattggatt
      181 tatctcagca acttattatc aatataaagg agtaaatatt tctaaaaata atattaaaaa
      241 agagaaaaaa tctatatcaa aaattgtttt taatttagta ttaatattat tgatttctta
      301 tatagagtca gttgcatttt cttcacatat aaatggtatg tttaatacgt cattttcatt
      361 ttatacaaca gcaattgctc atagagcagg agctgaaatg gctccagaga atactttggc
      421 agcaatagaa gaagcttcac atgctaaagc tgattatgca gaaatagatg ttcaagaaac
      481 taaagatgga gaacttattg tacttcatga ttcaaacttt aagagaacta caggagtaga
      541 taaaaatgtt tgggatgtaa attataatga agtaaagaca tatgatgcgg gaagctttta
      601 ttattatgga aaaggttatt atgatgaaaa gtttgttggg gaaaagatac caacactcaa
      661 agaaatgata aaatattcta aaggtaaggt gaaattatta atagaaataa aacttaatgg
      721 acatgaaaag ggagacgtag taaaaaaagt tgttcagtta attaaagaaa ataattttga
      781 aaatcaatgt atagttgcat ctatggataa aactatatta caaaaagtaa aaaaactgga
      841 tgagaattta ctgacatgct attttatggc aattgcatat ggtgattttt caaagttaaa
      901 ttatgttgat atatatggaa ttgaatcaac ttttgttaat aaaacagtag tagaaaagat
      961 tcataaaatg aacaaaaaaa tcttcgtttg gactgttaat agtgacagtt taattgagaa
     1021 aatgcttgac ctaaatgtag atagtattat tacagataat ccatacctta tagagagtgc
     1081 aatttattgg aagaaaaatg gatttattag tcaagtgtca gattatttat tttaaatatt
     1141 ctataattgt attatccagt aaaagagata ttattaaaat caataatatc tctttttaac
     1201 ataaataagg ttgttaaatt aaggctattc ttataaaaat tgtgtttatc agatataaat
     1261 aaggaataaa agtaaaaaaa ttgctatgac attattacaa ttaaaagagt tttaatatga
     1321 tagagaggta gattgtcaac tgtatattta ataaatttta ttatttatta gattttatat
     1381 aagtaaaata tatagatata taaatcttat gctagaatat atatgaaaaa caatatagat
     1441 ttggagggtt aaaagcatga cagagcgaga aaaaatgttg gcaggagaac tttacgattg
     1501 tggtgatatt gagttgttaa ctcaatggca taaagcaaaa gacctagtaa gagattataa
     1561 ccaaacagat tccttggatt tggaaaaaaa agaaaaaata ttaaatgagt tacttggtgg
     1621 taagggtaaa aacttatgga ttactgcacc gtattatgta gattatggaa ataatattta
     1681 ttttggaaat aattgtgaag tcaatatgaa ttgtacgttt ttagatgata ataaaattat
     1741 cattggggat aatgcactaa tcgcaccaaa cgtacaaata tatactgcat ttcatccaac
     1801 taatgctggt gatagatttg gtaatgccaa agaagatggt tcatttgaat tttgtaaaac
     1861 tcaaacagct ccagttacta ttggagataa tgtttggatt ggcgggggtg ctattatcat
     1921 gccaggtgtt actattggag ataatgtagt tataggtgca ggaagtattg ttacaaaaga
     1981 tattccaagt aatatgattg catacggaaa tccatgccgt attattcgag aaaataaata
     2041 aagttaaatt ttcttgcaaa atctcaaaaa acacctttct tactttatac atagggtatt
     2101 ttctaatact ttcacatcca tattcatttg ttaaattttt gtggaatgta gtttttatca
     2161 cttctatctt acatttccac cattttttta taaaagtcat tcctttatag atttatttat
     2221 aatagtatat attctatttg atgtttgaat aagcacttat ttttaagtaa aatactaact
     2281 tctaggttag tattttacta tagtatgatg attaagtaag aataaaatac tataatacat
     2341 aaagcttaat aaatataatt tttattttac ttagatattt tcttttgatt agtaatcaaa
     2401 agtggttata atttttttaa ttaataagag taacatttat tttatgaaag aggattatat
     2461 taatatatat tta
//
LOCUS       DAEPIN010000179         2463 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.184, whole genome shotgun sequence.
ACCESSION   DAEPIN010000179 DAEPIN010000000
VERSION     DAEPIN010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2463
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.184"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            306..1925
                     /locus_tag="KR250_003797"
     CDS             306..1925
                     /locus_tag="KR250_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HBH0069673.1"
                     /translation="MNIVISLIGGLGLFLYGMSLMGEGLQKSAGDKLKKIIELLTSNV
                     VMGVLVGTVVTGIIQSSSATTVMVVGFVNAGIMNLSQAIGVIMGANIGTTVTAQLVSF
                     NLEGIAPIALGIGILFYLFTSNQKTKHLSEILIGFGILFTGMEFMKDAVAPLAEYKAF
                     TDALLYFSKNPVLGILAGFAITGIVQSSSASMGMLIALASQGILPLSSALPILYGDNI
                     GTCVTSLLSSVGASRNARRAAVMHLSFNVIGTIIFMLVLNKPISAIVTHFDPTDTARQ
                     IANAHTLFNLTNVIILLPFSKYIVKLANRLIPIKETESEIVNNTKYLDERMFSTPSIA
                     LGNTVQEVVRMGHKATNSLEHSIAGFLNKSNEDINKAFESEKVVNKLQKDILNYLLKL
                     SKEPLRDDEHFRTDLLFNTVNDIERVSDHAENIAELAMSVKEMNISFSDSAIREIYEI
                     YNKTITNFKDALIVLDVKDFELANKVLEVENEVNYLEKTFRNSHMIRLNNGSCTIDAG
                     VLYLDLLTNLERISDHSTNIVKQVLKLKQKI"
     gene            2299..>2463
                     /locus_tag="KR250_003798"
     CDS             2299..>2463
                     /locus_tag="KR250_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HBH0069674.1"
                     /translation="MINYMVEIIYSEKISEVENMLKIENIQYEFTSRDGFIFFGFYDM
                     TDAYRFETLCS"
BASE COUNT          909 a          289 c          360 g          905 t
ORIGIN      
        1 atatcattct aaaaggatgt tattttttta catatttttt acaattatag ttaattcttt
       61 gttaaattgt ttgtacataa aatactaaaa atctaagttc aagtatttaa attgctatat
      121 atcataaatt ttctaaaatt aacaacaact taaaaattta acattctctt aacgtatact
      181 taacaaaaca ttaatatttt tattgacaaa atttgttatt ctatattatg gaaacaataa
      241 attttagtta ataaagttta taattcagat aaaaaaatta ttaatttctt aagggaggaa
      301 atatcgtgaa tatagtaatt agccttatcg gtggacttgg actttttctt tacggaatga
      361 gtttaatggg agaaggttta caaaaatcag caggagacaa attaaaaaaa attattgaac
      421 tcttaacaag taatgttgtt atgggtgtac tggtaggtac agtcgttaca ggtattatac
      481 aaagttcaag tgctactact gttatggttg taggatttgt aaatgcaggt ataatgaatc
      541 taagtcaagc tattggtgta ataatggggg caaatatagg tactacagtt actgctcaat
      601 tagtttcatt taatcttgaa ggaattgcac caattgcttt aggtataggt attttattct
      661 atttatttac atcaaatcaa aaaacaaagc atttatctga gatacttata ggttttggta
      721 tattatttac aggaatggag tttatgaaag atgcagtagc tccacttgct gaatacaaag
      781 cattcactga tgcactttta tattttagta aaaatcctgt attaggtata ttggcaggat
      841 ttgccataac tggtattgtt caaagttcaa gtgcatctat gggtatgtta atagcactag
      901 cttctcaagg aatacttcct ctatcatctg ctttacctat tctttatgga gataatatag
      961 gtacttgtgt tacttcactt ttatcaagtg ttggtgcaag tagaaatgct cgacgtgcag
     1021 ctgtaatgca tttaagtttt aatgtaattg gtacaataat atttatgctt gttttaaata
     1081 aacctatatc agccatagtt actcattttg acccaactga tacagctaga caaatagcaa
     1141 atgcacatac tttatttaat cttacaaatg ttataatatt attaccattt tctaaataca
     1201 tagttaaatt agcaaataga cttataccta ttaaagaaac tgaatcagaa atagtaaata
     1261 atactaagta cttagatgaa aggatgttta gcactccatc tatagcactt ggtaatactg
     1321 tacaagaagt tgtaagaatg ggtcataaag ctacaaactc tttggaacat tcaattgcag
     1381 gatttttaaa taagagcaat gaagatatta ataaagcttt tgagtcagaa aaagttgtca
     1441 ataagttaca gaaagatata ttgaactatt tgttaaaact ttctaaagaa cctcttagag
     1501 atgatgaaca ctttagaaca gatttgcttt ttaatactgt aaatgacatt gaaagagtct
     1561 ctgaccatgc agaaaatata gctgaattgg ccatgagtgt taaggaaatg aacattagtt
     1621 tttctgatag tgcgataaga gaaatatacg aaatatacaa taagacaatt actaatttta
     1681 aggatgcctt aatagtttta gatgttaaag attttgagtt agcaaataaa gttttagaag
     1741 ttgaaaatga agtaaattat ttagaaaaaa cttttagaaa ttcacatatg attagactaa
     1801 ataatggaag ttgtacaatt gatgctggtg tactttatct tgacttactt actaatctag
     1861 aaagaatttc tgaccactca acaaatatag taaaacaagt actaaaactt aaacaaaaaa
     1921 tatagtgatt atttttatat ataatcttaa tcaatgagaa gtaagaagct atagataatg
     1981 cttatatcat ttatggtttc ttatttttta tgtaatactc aaatttttat agtattctct
     2041 tattattaaa caatttctgc ctagaatatt ccatatatat atgacttctt tcataaaact
     2101 tgaactgccc ataactaaag tcacaagtat acataactat tttttaatca attataacca
     2161 atatttgttt tatttatctt cattttaatt cttcattcaa taatgtaata agattatttt
     2221 ttatttggat actattatat catctaaata tgttattata tatttggata ttttcatatt
     2281 aataggaggg attctacaat gataaattat atggtagaaa ttatatatag cgaaaaaata
     2341 agcgaagtag aaaacatgtt gaaaattgaa aatatacaat atgaatttac ttctcgtgat
     2401 ggctttatat tttttggttt ttatgatatg acagacgcat atagatttga gactttatgt
     2461 tca
//
LOCUS       DAEPIN010000180         2422 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.185, whole genome shotgun sequence.
ACCESSION   DAEPIN010000180 DAEPIN010000000
VERSION     DAEPIN010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2422
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.185"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            <1..864
                     /locus_tag="KR250_003799"
     CDS             <1..864
                     /locus_tag="KR250_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HBH0069675.1"
                     /translation="LFLDIQNLFLGIYELKSESVIFENTLDDNKIIAKMDTYIIIDYI
                     NASFEYLGYVLNGSALNSIDYGVPQNRERFVLIGAKKDFLKNKKIETPKPIVGENYVT
                     VRDAIGDLAEYEASKGSMDYCFEKNSNNIEKDFYRKIILDSNKIYNHVCTDTRNIALK
                     RFEYINQGNNFHSLPDELKGTYADPERTQNTIYKRLVYDKPSDTVVNVRKSMWIHPIK
                     NRAVSAREAARLQSFPDSYKFLGTKDSVYQQIGNAVPPLLGRVVAEKILNLFNDEPNE
                     YLRDVFEALDG"
     gene            complement(1864..>2422)
                     /locus_tag="KR250_003800"
     CDS             complement(1864..>2422)
                     /locus_tag="KR250_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class B sortase"
                     /protein_id="HBH0069676.1"
                     /translation="KILINIICIIALTFSSLSIYIKLSEYKKADEVYTELRENTINNS
                     KHQELYDKNNDYRFWLKINNVNINYPVVQGYNNDFYLTHDFYKNYLPLGSIFMDYRNN
                     FENDKSLIVYGHYMKNKTMFGQLENYTDEVFFKENNLVEINYKVQTYTYEIFSVYTAD
                     LINRDYLSIHFNNNDEFKYSLNYIT"
BASE COUNT          899 a          296 c          327 g          900 t
ORIGIN      
        1 ttattcttgg atattcaaaa tttattttta ggaatatatg agttaaaatc tgaatctgta
       61 attttcgaaa atacacttga tgataataaa attatagcta aaatggatac ttacattata
      121 atagattata taaatgcatc ctttgagtat ctaggatatg tattaaatgg aagtgcacta
      181 aattcaattg attatggagt acctcaaaat agagaaagat ttgtattgat aggagcaaaa
      241 aaagacttct taaaaaataa aaagatagaa actcctaagc ctatagttgg agaaaattat
      301 gttacagtaa gagatgcaat aggcgattta gctgaatatg aggcttctaa aggtagtatg
      361 gattattgtt ttgaaaaaaa tagtaataat attgaaaaag atttttatag aaagataata
      421 ttggatagca ataaaatata caatcatgta tgtacagata caagaaatat cgcactaaaa
      481 agatttgaat atataaatca aggaaataat tttcattctt tgccagatga gttaaaagga
      541 acttatgctg atccagaaag aactcaaaat actatttata aaagactagt atatgataag
      601 ccatcagata ctgttgtaaa tgtaagaaaa tctatgtgga tacatcctat taaaaacaga
      661 gctgttagcg caagagaggc ggctagattg cagtcattcc ctgatagtta taagttttta
      721 ggtacaaaag attctgtata tcaacagata ggaaatgcag taccaccttt attaggtcga
      781 gtagtagcag aaaagatatt aaatttattt aatgatgaac caaatgagta tcttagagat
      841 gtgtttgaag cactagatgg atagttcaaa taaaatagtg ggtattattc ttgtgattct
      901 tatccaacat taatccacga aataaggact gtcgaatgag taattttaaa ttgctgtgcg
      961 acagttcttt ttttacttgc ttttcattaa tttatattta tgcaaaattt gagcttgaaa
     1021 acggtcataa gaaataaagg tataaaaata caattttaaa atagaataat ttacaaagtg
     1081 taagattaaa aaatattttt gagaggatga atatacatac tacatttatt aatgattatc
     1141 ttatttgtga aacttagaaa tactataggc taacactaca ggtataaact ctgaaagaat
     1201 attttgattg cctttgatta gaattctttt aaagttcata ctatgtttta tttgggtgaa
     1261 agctccttat gcctgaatac ttctattcat tctaagttca catccttgtg taccagtgat
     1321 tctttctaaa ttatcttaat gccaaaatct tgttcgcatt tcaaaataaa gtcaggtagc
     1381 tacttcaaca ttttagttat atttcttcaa tagtttgtct ccaaacaaag gtacatctat
     1441 tagaaacaga tttaatttta tttatatcaa taaataatat atactttgaa acttcattaa
     1501 tatcaccaag ataatttata agttaagcta ttatatacta tcttcaccaa ctacatcctc
     1561 catagtttta cactttctta attcagcttg gtaatctaaa tctgtaataa ttatttctct
     1621 ttgatttttc atttctttgc ctacaaaagt ttaatataaa tattattcca tacttaccaa
     1681 ttttacgttc aaaaaagact gtagataagt ctgcatttac acaaacctac ttataggtct
     1741 cttttttgaa attaatttag catgaactat agtaagagca tctttaaatt catagttaca
     1801 agtatacaaa gttataattt tatctgaaat atctaccttt atatcagatt tgtgtactga
     1861 tttttaggtt atataattca aagaatattt aaactcatca ttattattaa agtgtatact
     1921 taaataatct ctgttaatta agtcagcagt atatactgaa aaaatttcat aagtataagt
     1981 ttgtacttta taatttattt ctactaagtt attttctttg aaaaatactt catcagtata
     2041 gttttctagt tgaccaaaca tagttttatt tttcatataa tgaccataga ctattaaact
     2101 cttatcattt tcaaaattat ttctataatc catgaaaata ctacctaaag gaagataatt
     2161 tttataaaaa tcatgagtta ggtagaaatc attgttatat ccttgtacta caggatagtt
     2221 tatatttacg ttgtttattt taagccagaa tctataatca ttatttttat cataaagttc
     2281 ttgatgtttg ctattattga tagtgttttc tctgagttcc gtatatactt catcagcttt
     2341 tttatattca gataatttta tgtaaatcga aaggctagag aaagttagcg caataatgca
     2401 aataatattt attaatattt tt
//
LOCUS       DAEPIN010000181         2174 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.186, whole genome shotgun sequence.
ACCESSION   DAEPIN010000181 DAEPIN010000000
VERSION     DAEPIN010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2174
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.186"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            complement(322..1500)
                     /locus_tag="KR250_003801"
     CDS             complement(322..1500)
                     /locus_tag="KR250_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBH0069677.1"
                     /translation="MKYHIFLIITTILIIELCNIYICKIASFRNKNITIHKNYKYIFL
                     IKPLTFLIIIFTKLPLPLYYILFYIVSHKLLLSRKSSTNNHFIPNMAVISFCILHLIT
                     VSTISLVKNTSPDIIFANMTYKLISILIASILDFILWYSMNKIKEHIYIITQNQIEFS
                     LFLKFIWFTLGYILFDSIQITFAPPSSLTSYFILGSNILLILQLIIFINHIYSIEKNI
                     YLEKEHNRLKMIKQEQLLKVNLLKKKANIDELTSAYNRKFAFEKIQHYIDNNLLFSLI
                     YIDLDKLKTINDTYGHLAGDNYIINFSSIVIENLGIDDIFARIGGDEFIVIMPKSNEN
                     TTKEFIEKISKIMDNTISFSYGIVEVSSENKMTVEEIINIADIRMYTNKQNNHKRNNN
                     "
BASE COUNT          813 a          200 c          278 g          883 t
ORIGIN      
        1 aaaaatatat ttattgctaa accaatgatg gagcagttta gtgaataaaa aatgtttttt
       61 attgtggaga ttttataagt tactttaatt tgtatgaata aaatcaacat aaaaattgtc
      121 caatttataa ttagatgaaa ctttaaataa gtacaaataa taaaaattaa ataattaaat
      181 atagaatata taatatatac tgtgtattta tttttagagt tattaatcat tatgtccaga
      241 tatttaaaaa aattataatg atagagataa taagataaaa aacatagtga gactacaagt
      301 aagctcatgt aaatattcat tttagttatt gttcctttta tgattatttt gtttattagt
      361 gtacattcta atatcagcaa tattaataat ttcttctaca gtcattttgt tttcgctaga
      421 aacttctaca ataccatagc taaaagatat cgtattatcc ataattttac ttattttctc
      481 tataaattct tttgtggtgt tttcattaga tttaggcata ataactataa actcatctcc
      541 tcctattcta gcaaatatgt catctattcc taagttttca ataactatac ttgagaagtt
      601 aataatatag ttatccccag ctaaatgtcc atatgtatca tttatcgtct ttaatttatc
      661 caaatcgata tatatcaatg aaaataataa attgttatca atgtaatgct gtattttttc
      721 aaaagcaaat tttcgattat atgctgatgt taattcatca atgttggctt ttttctttaa
      781 taaatttact tttagtaatt gttcttgttt tatcattttt agtctattat gttctttttc
      841 caaataaata tttttttcaa tggaatatat atgattgatg aatataatta gttgtaagat
      901 taataatatg ttacttccca atataaagta ggaagttaga gaacttggtg gagcaaatgt
      961 aatttgtata ctatcaaata atatatatcc taaagtgaac cagataaatt ttaaaaatag
     1021 actgaactct atttgatttt gagttattat ataaatatgc tcttttattt tattcataga
     1081 gtaccataag ataaaatcta aaatagaagc tattaaaata ctaattaact tataggtcat
     1141 atttgcaaat attatatctg gacttgtatt tttaacaagt gatattgtac ttacagtaat
     1201 aaggtgtaaa atacaaaaac ttattactgc catattggga ataaaatggt tgttagtaga
     1261 actttttctg cttaagagca atttatgact tactatgtaa aacaatatat aatatagtgg
     1321 taatggtaat tttgtgaata ttattatcaa gaaggttaat ggttttatta aaaaaatata
     1381 tttgtaattc ttatgtatag ttatattttt gtttctaaaa gatgctattt tgcatatgta
     1441 aatgttacat agttcgatta ttaatatagt agttatgatt aaaaatatat ggtatttcat
     1501 agtacatcct cttttaaagt gaaattgtat ttacttaaaa tagattagat tttatatatt
     1561 ctattttaca gtttttattt aggtacatgc aaagagctta ttatggatac cttctttttt
     1621 attgcatgtg tctgtgttat ataaaattta aaaagtatta tatcttaata tacagtatgt
     1681 atgataacta tgtgtccaaa tatagtaatt atgtatggaa agtataatag ttatgtgtat
     1741 aagtatagta attacatata gcatgtatga tgactatgtg tccaagtata gtaattatat
     1801 atagtatata tgataactat gtgtataagt atagtaatta catatagtat gtatgataat
     1861 gtgtccaagt atagtaatta tatagtatgt atgataacta tatgtccaag tatagtataa
     1921 ttacacatag caaatataat agttatgtgt ccaagtatag taattacata tagtatgtat
     1981 gataactata tgtccacgta tagtataatt acatatagta aatatagtag ttatgtgtcc
     2041 aagtgtagta attatatata gcatgtataa taactatgtg tataaagtat agtaattaca
     2101 tatagcaagt ataatagtta tgtgtccaag tatagtaatt atatatagta agtataataa
     2161 ttatgtgtat caaa
//
LOCUS       DAEPIN010000182         2082 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.187, whole genome shotgun sequence.
ACCESSION   DAEPIN010000182 DAEPIN010000000
VERSION     DAEPIN010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2082
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.187"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            523..1263
                     /locus_tag="KR250_003802"
     CDS             523..1263
                     /locus_tag="KR250_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HBH0069678.1"
                     /translation="MKNFRDLGGNKTEDGRTVKKGLFYRSAKLSNLSENDIKILKELN
                     IKYIFDYRSDEEARKHPSTIISNIKNIRIPAMRELEESGGSFGSIEDMIDGLFEKDGA
                     FNMLNNSYYNLPINNPSYKKLVELIRDYSNLPILNHCTAGKDRTGVGSAIILMILGVS
                     RENIMKDYLKSNDFADKEIERFIEYKPKFKDIPKENLKYIFGVNEEYMKTAFRRIDEE
                     YISVEAYLYGEFNLNKEEIRKLRNQYLE"
     gene            1275..>2082
                     /locus_tag="KR250_003803"
     CDS             1275..>2082
                     /locus_tag="KR250_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HBH0069679.1"
                     /translation="MSLNITARQINIIKILLNSKDTISGIALSQEIGCSSKTIQNEIK
                     DINKQLRNGKILSIRGVGYKLEGSFDEVNLNSNLYDDVDRVEYIIRKILTLSNEEKNT
                     IKLEDLADSMYVSVSTVKNDLKEVKRMLEKYSISVKSKHKQGICVLESEDKILNCIVD
                     LSNKRDNQLSLNDFLNDDIKERKLLIKKLLLDILNENGLVLTDIEFKSVLNNILVMLS
                     RHKYNEKEFIENYIENYRSKRSSILENEQNKEIIINSIKTFCKNLKLATSI"
BASE COUNT          944 a          150 c          321 g          667 t
ORIGIN      
        1 gctaactaat aaaaatatac acaacccata aaaaattaaa agtattacta tagaaataag
       61 tgtacgattt aaatttgtat tatttgtaat aagtcttata ataggtctta agatggatgc
      121 aatgataaaa ctgattacaa aaggcattaa taatggcaat aagaatttta atacaatata
      181 tacaatactt gttattaaaa ctacatatgt aaatttaatt ataaaattct tttgcttttg
      241 aatatccata atataaaacc tccaatgtat ttgatattat tctaaatatg gtctttttta
      301 cagaaaatat actactgtat attgattata aactataaag cttatgtaaa gtaaataaat
      361 tatgtaaaat ttaggttaat tttgtataaa gagatacttt tatattttac aattaataac
      421 caaatactaa aaaatatttt aaataaagaa cttaaaattg tgataaaatt aatttataga
      481 tattaaaaaa agtataatat atggttttta agaggtgcta agatgaaaaa ttttagagat
      541 ttaggtggga acaaaactga ggatggcaga actgtaaaga agggattatt ttataggtct
      601 gcaaaattat caaatctaag tgaaaatgat ataaagattt taaaagagtt aaatattaaa
      661 tatatatttg attatagaag tgatgaagaa gctcgtaaac atccatcaac aataatatca
      721 aatataaaaa atataagaat accagcaatg agagagctag aagaatcagg aggaagtttt
      781 ggttctattg aagatatgat tgatggattg tttgaaaaag atggtgcatt taatatgtta
      841 aataatagtt actataattt gcctataaat aatccgtcat ataaaaaatt agtagaactt
      901 ataagagact attctaattt accaatatta aatcattgta cagctggtaa ggatagaact
      961 ggtgtgggga gtgctataat tcttatgatt ttaggggtat cgagagagaa tataatgaaa
     1021 gattatctaa aaagtaatga ttttgcagat aaagagatag aaagatttat agaatataag
     1081 cctaaattta aggacattcc aaaagaaaat ttgaaatata tatttggtgt aaatgaagag
     1141 tatatgaaaa ctgcatttag aagaattgat gaagaataca ttagtgtaga agcatattta
     1201 tatggagaat ttaatttaaa taaagaagaa ataagaaaat taagaaatca atatctagag
     1261 taaaaggtga gtttatgagt ttaaatataa cagcaagaca gataaatata ataaaaatat
     1321 tgttaaattc aaaagataca attagtggta ttgccttaag tcaagagata ggatgttctt
     1381 caaaaactat acaaaatgaa ataaaggata ttaataaaca acttagaaat ggtaaaattt
     1441 tgtcaataag aggagttggt tataaactag aaggaagttt tgatgaggta aatctaaata
     1501 gcaatctcta tgatgatgta gatagagtag aatatataat cagaaaaata ttaaccttaa
     1561 gtaatgaaga aaaaaatact ataaaacttg aagatttagc cgattctatg tatgtaagtg
     1621 tttcaacagt aaaaaatgat ttaaaagaag taaaaagaat gttggaaaag tattcaatat
     1681 cagttaaatc taagcataaa caaggtatat gtgttttaga aagtgaagat aagatattaa
     1741 attgtatagt tgacttaagc aataagagag ataatcaatt gagtttaaat gattttttaa
     1801 atgatgatat aaaggaaaga aaactattaa taaaaaaatt attattagac atattaaatg
     1861 aaaatggatt agtattaact gatatagaat ttaagagtgt gttaaataat atattagtta
     1921 tgttaagcag acataagtat aatgaaaaag aatttataga gaattatata gaaaattata
     1981 ggagcaaaag aagctcaatt ttagaaaatg aacaaaacaa agaaattata ataaattcaa
     2041 taaaaacatt ttgtaaaaat ttaaaattag ctacatcaat ag
//
LOCUS       DAEPIN010000183         2054 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.188, whole genome shotgun sequence.
ACCESSION   DAEPIN010000183 DAEPIN010000000
VERSION     DAEPIN010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2054
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.188"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            27..167
                     /locus_tag="KR250_003804"
     CDS             27..167
                     /locus_tag="KR250_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069680.1"
                     /translation="MEKSNTTVTKKEKIEDIGMKLEHLLSEDITFYELLLEILYKEKE
                     EN"
     gene            complement(164..535)
                     /locus_tag="KR250_003805"
     CDS             complement(164..535)
                     /locus_tag="KR250_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0069681.1"
                     /translation="MESLGDRIANLRKELDINQKELATKVGITEASLSRYENNLREPK
                     SEIIVRLAKALETSTDYLLGVNDNTKISKEDKLIIENLSVSEKTKKLLEKIYSLEKED
                     REAIEKMIDNAYLKRFLKEEN"
     gene            1084..1341
                     /locus_tag="KR250_003806"
     CDS             1084..1341
                     /locus_tag="KR250_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069682.1"
                     /translation="MIIIRSQDKTNLMQINQIKIDGSQVYAVFESKIDTVKIGDYENN
                     TRAIQVLDNIQNFIENGTKYDYITSNKVRYNVNKIFQMPAK"
     gene            complement(1451..>2054)
                     /locus_tag="KR250_003807"
     CDS             complement(1451..>2054)
                     /locus_tag="KR250_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBH0069683.1"
                     /translation="NLKVEKNVMIHLVWVYIFLLYIYLALSKAGIGSIWDIGRYSGLF
                     RIDEINLIPFDSVGILTYILNIIMFMPLGFLLPLIWKNFRNIINVSLTGLGFSLAIEL
                     CQLFNLRATDIDDLMMNTLGAVLGYFIWVGVNNLFNLIKKKDIFPSIYKNIIQDEIAI
                     TIDDDISEKITSLYKNEAIIYLILAVLGEFLLFNWSIIPY"
BASE COUNT          831 a          235 c          239 g          749 t
ORIGIN      
        1 cgtaagtttg ttgggggatg gtatatgtgg aaaagtcaaa cactactgtt acaaaaaaag
       61 aaaaaattga agatatagga atgaaattag aacatttatt gagtgaagat attacttttt
      121 atgaattatt acttgagatt ttgtataaag aaaaagaaga gaattaattc tcttctttaa
      181 ggaatctttt taaataggca ttatcaatca ttttttctat agcttctcta tcttcttttt
      241 caagagaata tattttttct aagagctttt ttgttttttc acttactgat aagttctcta
      301 tgattaattt atcttctttg cttatttttg tattatcatt tacgcctaat aaataatctg
      361 ttgatgtttc taatgctttt gctaatctaa ctattatttc acttttaggt tctcttaaat
      421 tattttcata acgtgacaag ctagcctcag ttataccgac tttagtagca agttcttttt
      481 gatttatatc taattctttt cgtagatttg ctattctatc tcctaaactt tccatatgta
      541 catcactcct taccgaaatt ataagttatt ttgataaaaa gtaaaataaa attaccaaaa
      601 atataaaaaa aaaaggttaa aataataaaa tatattgaaa attaccattt gtatatgtat
      661 aatatgaatt atacaaatgg taatttgatg agaggtggta tgttgaaata agaaactgaa
      721 ttaattaata aatacataga aagttttgaa aacctttaat tgaaatataa acaatatgta
      781 caaaattttt tgcaagtaaa attaccaaat gtataaaata aatcagtagc atagataatt
      841 ttatgcttta atacattaga ttatttaatt tagttataat ggtcaaaaat gcaatctaca
      901 aataaaacaa agtattatca aaaaagtatt ctttaagaaa attttttatt agagatgtaa
      961 ttgatataaa taatcgtatt aaaaaataaa atactaagtt taaaaattta aataatctat
     1021 aaatcaatta taaaatatta tttcttagaa attaaaatga attaagtaaa tggaggaatt
     1081 gatatgataa taattagaag tcaagataaa actaatttaa tgcagataaa ccaaattaaa
     1141 atagatggta gtcaagttta tgcagtattt gaaagtaaaa ttgatactgt aaaaatagga
     1201 gattatgaaa ataatacaag agctatacaa gtgttggaca atatacaaaa cttcattgaa
     1261 aatggaacaa aatatgatta tataacatca aataaagtca gatacaatgt taataaaata
     1321 tttcagatgc cagctaaata gtgagtgtgc attttaattt aataactaaa gcccactttc
     1381 ttttgtggta tgaaagaggg ctttacatat ttgtaaatat cgaagtacac ttgttttatc
     1441 aataaattat ttaataaggt ataatggacc aattaaacaa taaaaattct cctaaaacag
     1501 caagtattaa atatataata gcttcatttt tgtaaagaga ggtaattttt tcgcttatgt
     1561 catcatcaat tgtaatagca atttcatctt gaataatatt tttatatata gatgggaaaa
     1621 tatctttttt ctttataaga ttaaataaat tatttacacc tacccatata aaatatccta
     1681 aaacagcacc taatgtgttc atcattaaat catcaatatc tgtagctctt aagttaaaca
     1741 attgacaaag ttcaattgct aatgaaaaac ctaatccagt taatgaaaca ttgataatat
     1801 ttctaaaatt tttccaaatt aaaggtagta aaaatcctaa aggcataaac ataattatat
     1861 ttaaaatata tgttaagatg ccaactgagt caaaaggtat tagatttatt tcatcaattc
     1921 taaaaagccc actatacctt cctatatccc atatgctacc aattccagct ttactaagtg
     1981 ctagatagat atataataaa aaaatatata cccatactaa atgtatcatt acattctttt
     2041 caactttcaa attt
//
LOCUS       DAEPIN010000184         1986 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.189, whole genome shotgun sequence.
ACCESSION   DAEPIN010000184 DAEPIN010000000
VERSION     DAEPIN010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1986
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.189"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            84..200
                     /gene="rrf"
                     /locus_tag="KR250_003808"
     rRNA            84..200
                     /gene="rrf"
                     /locus_tag="KR250_003808"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            207..281
                     /locus_tag="KR250_003809"
     tRNA            207..281
                     /locus_tag="KR250_003809"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:239..241,aa:Asn,seq:gtt)
     gene            286..360
                     /locus_tag="KR250_003810"
     tRNA            286..360
                     /locus_tag="KR250_003810"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:319..321,aa:Glu,seq:ttc)
     gene            366..441
                     /locus_tag="KR250_003811"
     tRNA            366..441
                     /locus_tag="KR250_003811"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:399..401,aa:Val,seq:tac)
     gene            447..523
                     /locus_tag="KR250_003812"
     tRNA            447..523
                     /locus_tag="KR250_003812"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:481..483,aa:Asp,seq:gtc)
     gene            534..608
                     /locus_tag="KR250_003813"
     tRNA            534..608
                     /locus_tag="KR250_003813"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:566..568,aa:Thr,seq:tgt)
     gene            623..707
                     /locus_tag="KR250_003814"
     tRNA            623..707
                     /locus_tag="KR250_003814"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:657..659,aa:Tyr,seq:gta)
     gene            717..790
                     /locus_tag="KR250_003815"
     tRNA            717..790
                     /locus_tag="KR250_003815"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:749..751,aa:Gly,seq:tcc)
     gene            801..877
                     /locus_tag="KR250_003816"
     tRNA            801..877
                     /locus_tag="KR250_003816"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:835..837,aa:Arg,seq:tct)
     gene            887..962
                     /locus_tag="KR250_003817"
     tRNA            887..962
                     /locus_tag="KR250_003817"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:920..922,aa:Gln,seq:ttg)
     gene            974..1049
                     /locus_tag="KR250_003818"
     tRNA            974..1049
                     /locus_tag="KR250_003818"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1007..1009,aa:Lys,seq:ttt)
     gene            1052..1140
                     /locus_tag="KR250_003819"
     tRNA            1052..1140
                     /locus_tag="KR250_003819"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1086..1088,aa:Ser,seq:tga)
     gene            1158..1248
                     /locus_tag="KR250_003820"
     tRNA            1158..1248
                     /locus_tag="KR250_003820"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1192..1194,aa:Ser,seq:gct)
     gene            1257..1333
                     /locus_tag="KR250_003821"
     tRNA            1257..1333
                     /locus_tag="KR250_003821"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1291..1293,aa:Pro,seq:tgg)
     gene            1419..1494
                     /locus_tag="KR250_003822"
     tRNA            1419..1494
                     /locus_tag="KR250_003822"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1452..1454,aa:Trp,seq:cca)
     gene            1555..1631
                     /locus_tag="KR250_003823"
     tRNA            1555..1631
                     /locus_tag="KR250_003823"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1589..1591,aa:Pro,seq:tgg)
     gene            1638..1714
                     /locus_tag="KR250_003824"
     tRNA            1638..1714
                     /locus_tag="KR250_003824"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1672..1674,aa:Ile,seq:gat)
     gene            1718..1793
                     /locus_tag="KR250_003825"
     tRNA            1718..1793
                     /locus_tag="KR250_003825"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1751..1753,aa:Phe,seq:gaa)
     gene            1801..1877
                     /locus_tag="KR250_003826"
     tRNA            1801..1877
                     /locus_tag="KR250_003826"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1835..1837,aa:Met,seq:cat)
BASE COUNT          517 a          452 c          531 g          486 t
ORIGIN      
        1 taactctaaa aaaaaataaa aaaagtattg acaaaaatag taaaaaatgt taatattata
       61 cttgtcctag caacaagaaa aaatgtggtt ataatagcgg aggagataca cctgtttcca
      121 ttccgaacac agaagttaag ttctctagcg ctgatggtac ttggtgggaa actgcctggg
      181 agagtaggac atagccacgt gaatgttcca aggtagctca acggtggagc aaccggctgt
      241 taaccggtag gctgtgggtt cgagccccac ccttggagcc attatggctc gttggtcaag
      301 aggttaagac accgcccttt cacggcggta acaggggttc aattccccta cgagtcacca
      361 tttgtgggat catagctcag ctgggagagc acctgcctta caagcagggg gtcacaggtt
      421 cgagccctgt tggtcccacc atttgcggcc tggtagttca gttggttaga atgccagcct
      481 gtcacgctgg aggtcgaggg ttcgagcccc ttccaggtcg ccaaaataaa aatgctggtg
      541 tggctcaacg gtagagcagc tgacttgtaa tcagcaggtt gtaggttcga ttcctatcac
      601 cagctccatg taaaactaat acggaggatt tcccgagcgg ccaaaggggg cagactgtaa
      661 atctgttgtc atcgacttcg gtggttcgaa tccaccatcc tccaccaatt aaatatgcgg
      721 gtgtagctca atggtagagt tctggccttc caagccagct gtgagggttc gatccccttc
      781 acccgctcca aagaaagcgt gggtgcatag ctcagctgga tagagcaacg cccttctaag
      841 gcgtgtgtcc ggggttcgaa tccctgtgcg ctcaccacat atatattggg gattagccaa
      901 gtcggtaagg cacatgactt tgactcatgt atgcgtaggt tcgagtcctg catccccagc
      961 caaataaaaa tatggtctat tagctcagtc ggtagagcac ctgactttta atcagggtgt
     1021 cccgcgttcg agtcgcggat agatcaccaa tggagaggtg tccgagtggt ttaaggagct
     1081 ggtcttgaaa accagtgact tcgaaagggg ccgtgggttc gaatcccacc ctctccgcca
     1141 aaaaaatgac tgaatttgga gaagtactca agtggctcaa gaggatcccc tgctaaggga
     1201 ttaggctggg taaccggtgc gagggttcga atccctcctt ctccgccatt caaaaacgag
     1261 gtgtagcgca gtttggtagc gcacatggtt tgggaccatg gggccggggg ttcgagtccc
     1321 ttcacctcga ccaacttaag cttttagttc agttggttag agtgcccaac tcatggctag
     1381 taattctagg gtttgagtcc atgaaggtta ttcttcatag gggtgtagct cagttggtag
     1441 agcgtcggtc tccaaaaccg tgcgccgggg gttcgagtcc ctccacccct gccaaataaa
     1501 aaggacttga aaaaagttgg attttagagt agtataagat agtaaataag atttcgaggt
     1561 gtagcgcagt ttggtagcgc acatggtttg ggaccatggg gccgggggtt cgagtccctt
     1621 cacctcgacc aagaaaaggg cctatagctc aggtggttag agcgcacgcc tgataagcgt
     1681 gaggtcgctg gttcgagtcc agctaggccc accatttgcc cagatagctc agtcggtaga
     1741 gcaggggact gaaaatcccc gtgtcggtgg ttcgattccg cctctgggca ccaggaatac
     1801 ggcggtatag cttagttggc tagagcgttc ggttcatacc cgaaaggtca taggttcgac
     1861 tcctattacc gctaccatct aactatatca ataaagaact ttgaaaatta aacagtaggt
     1921 taatttataa acaagaaaca aaccataaag ccagatattt tgataacaat agtatctgag
     1981 cctgat
//
LOCUS       DAEPIN010000185         1804 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.190, whole genome shotgun sequence.
ACCESSION   DAEPIN010000185 DAEPIN010000000
VERSION     DAEPIN010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1804
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.190"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            <1..534
                     /locus_tag="KR250_003827"
     CDS             <1..534
                     /locus_tag="KR250_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069684.1"
                     /translation="LLSFNEKKIEKHKCKNYHNDSSKDKYKYSQSEFIAATLKEADDM
                     NEEERRILEAEKRILETEKRVHQDNKEVKESIDKQFNDIKGIFKEYKNDLDKDRIELK
                     NDLARERAELKSDLKEAINEHKKVTEKDVSEIKNSMKGIEDRIDSTNKWIIGLCITTI
                     IGIATMAITIGISIWPK"
     gene            complement(944..1132)
                     /locus_tag="KR250_003828"
     CDS             complement(944..1132)
                     /locus_tag="KR250_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069685.1"
                     /translation="MTKTILCDYCNKGINKDDNKYITFHKKSHMKTNICINCALNLID
                     KDKLNENIINNQHDYSKK"
     gene            1390..1533
                     /locus_tag="KR250_003829"
     CDS             1390..1533
                     /locus_tag="KR250_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069686.1"
                     /translation="MSEFLLGVLASLTASFITYIISRKVKSHSGRSDFELDVKIKFNK
                     KRH"
     regulatory      complement(1735..>1804)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
BASE COUNT          755 a          202 c          239 g          608 t
ORIGIN      
        1 ttattgagtt ttaatgaaaa aaagatagaa aaacataaat gtaaaaatta tcataatgat
       61 tcttcgaaag ataaatacaa atatagtcaa agtgaattta tagctgctac tttaaaggag
      121 gcagatgata tgaatgaaga agaaagaaga atactagaag cagaaaaaag aatattagag
      181 actgagaaaa gagtgcatca agataacaag gaagtaaaag aaagtataga taagcaattt
      241 aatgacataa agggtatttt taaagaatac aagaatgatt tagacaaaga tagaatagaa
      301 ctaaaaaatg atttagctag agaaagagct gagttaaaaa gtgatttaaa agaagctata
      361 aacgaacata aaaaggtcac agaaaaggat gtaagtgaaa taaagaatag tatgaaaggt
      421 atagaagata gaattgattc aactaataaa tggattattg gactatgtat aactactata
      481 ataggaattg ctacaatggc tattacaata ggtatatcta tatggccaaa gtaacacaaa
      541 aagaggtaac tagaattaaa atagttatct ctttttatac ttaaacaaaa cattgaaaaa
      601 atagtaaaaa atattaacag gatacataaa actatagtat aacctgtaat aataaaacga
      661 acgaaaatta tcatactaaa acataagaca tgctataatt gtattagata aatgcttgaa
      721 tatataccaa aagtactctt tttataagag tgcttatttt tttgaaattc atcaacatat
      781 aaacaatcaa gaacattact caacacacct taaaattgat ttaaattctt tttcatacac
      841 aaagttatat gatataataa aaaagtaaat atgtaacccc aacacatctt tactaagtca
      901 aacattatta tatagagcat tcttcattat ggagagtgct ttttcatttc tttgaataat
      961 catgttgatt atttataata ttttcattta atttatcttt atctatcaaa tttaatgcac
     1021 aattaataca aatgttagtt ttcatatgac ttttcttatg aaaagtaata tacttattat
     1081 catctttatt tattccttta ttacagtaat cacataatat agtcttagtc atatttttat
     1141 tcctttcatt ttatattttc cataattaag tttaacatat ttagtatata cctatgtata
     1201 tatatttaaa acaatgtttt ttattgcaat atagaatata ttgtataaat aaaaaaaatg
     1261 gagaatagat atttgataat tgtaaaatta tgttataatt aaaatgcaag aataatcttg
     1321 tggaactaca atctaagagt ggagcttcat tttctacatt ccatccctta aaaggaagga
     1381 ggtggagaaa tgagcgaatt tttactagga gtgttagcta gtttaacagc tagctttatt
     1441 acatatatta tttccagaaa agtaaaaagc cactctggca ggagtgactt tgagcttgat
     1501 gtaaaaatca agtttaataa aaaacgacat taatatttag ttaatgaaac ttcactctag
     1561 gctaatagat tgtagtttct ttttttgttt ttgatacgaa tttacatctt tattatatcg
     1621 cattttaaga aaaaataaaa ctgtgaatgc tagaaatata ttttatttta tctctaagta
     1681 tatatattat tatcccccta aaaaactttc ataaaaacaa aagaacaata tctctttggc
     1741 aactggctga cataactcat aagatattta tataagtctt agtccctata gctttgcgtc
     1801 acta
//
LOCUS       DAEPIN010000186         1749 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.191, whole genome shotgun sequence.
ACCESSION   DAEPIN010000186 DAEPIN010000000
VERSION     DAEPIN010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1749
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.191"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            266..946
                     /locus_tag="KR250_003830"
     CDS             266..946
                     /locus_tag="KR250_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0069687.1"
                     /translation="MQIDVLIIDGDKNSSQELKNFLEGKGINVYLAYNCKDAIIKIFS
                     KKFDLIFLEIILVDGDGWDFCKKVRSITTCPIIHITYINENQSILNALNSGGDDYLIK
                     PLNLEILYAKVKAILRRRDLFLNNNKNNIKLEDYNRASGVIKLENKMIKLTPTENRLL
                     NYFIENAEKTLTIKEIYEHVWMNEYLDDNYSVVVAVSGLRKKIEKDYKNPQKIITIRE
                     VGYYFNKI"
     gene            1393..1677
                     /locus_tag="KR250_003831"
     CDS             1393..1677
                     /locus_tag="KR250_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069688.1"
                     /translation="MLVYNKSFYPNDIFPRLDFSKIKKQLKLIDNDLSDFGSICIIEK
                     EHYTISVNSIGEINVYYDLEYENKVYRIVYEIEKLFKSQVGRFSISTYRN"
BASE COUNT          776 a          109 c          271 g          593 t
ORIGIN      
        1 tatatgctta tctttataca gtaaataaaa aatcaaatga aataccaaat aaagtgtata
       61 tcttaaaaca atttactaga gatacaagga tgctatgtga attttgaagt gtgcagttta
      121 gataataaat atatataaat aataaagaaa atggatttat agtatttaaa atttcttata
      181 agggtaaaaa atgctattag aaaatgtact aaaaatattt ttccaattct ttatagaaaa
      241 ataatgatat ttaggagaga ttgtaatgca aattgatgta ttgataattg atggagataa
      301 gaatagttct caagagctga aaaatttttt agaaggaaag gggataaatg tctatttagc
      361 atataattgt aaagatgcta ttataaaaat atttagcaag aaatttgact taatattttt
      421 agagataatt ttagttgatg gtgatggttg ggatttttgt aaaaaagtta gaagtataac
      481 tacttgccca attattcata taacttatat aaatgaaaat caaagtattt taaatgcttt
      541 aaattctgga ggagatgatt atttaataaa gcctttaaat ttagaaatat tatatgcgaa
      601 agtgaaagct attttaagaa gaagagattt atttttaaat aataataaaa ataatataaa
      661 actagaagat tataatagag ctagtggagt aataaagtta gaaaataaaa tgataaagtt
      721 aactccaaca gaaaatagat tattaaatta ctttatagag aatgctgaaa agactctaac
      781 tattaaggag atatatgaac atgtatggat gaatgagtat ttagatgata attatagtgt
      841 agtggttgct gtgagtggat taagaaagaa aatagagaaa gattataaaa atcctcaaaa
      901 aattataacg ataagagaag tagggtatta ctttaacaag atataaagct ttataaacag
      961 aatgaatgat aaggcataga taatatgtta gtatattaca agagtttttg ttctaacgat
     1021 atatttgtaa aattagattt aatttactta attaaattat agcagtagaa taaatataaa
     1081 aaatgtctga aatattatag acatcagtaa aatatataaa actataatat aagtcgtaat
     1141 aataaaacaa atgaatatta attaataaaa agatagtagc tatttttata tagctattat
     1201 ttttttattg ttgtggatga aaatatactt atattttact aatatacatt taaaaattac
     1261 aatttaaagc attaaaaaat aaatttgtat tttgttttat ataaagtaat ttgaaaaata
     1321 tggaaaaatt gattagtaca ttattaatta gggtataaaa tggtgtaaat agaatgataa
     1381 ggggtggatg aaatgcttgt atataataaa agtttttatc ctaatgacat atttccaaga
     1441 ttagattttt caaaaataaa aaaacagtta aaattgatag ataatgacct gtcagatttt
     1501 ggaagcatat gtataataga aaaagaacat tatacgataa gtgtaaacag tataggtgaa
     1561 ataaatgtgt actatgattt agagtacgaa aataaggtgt atagaatagt ttatgagatt
     1621 gaaaagttat ttaaatctca agttggaagg tttagcatat ctacatacag aaattgataa
     1681 ttaaaaaagt agaaattaaa aaacttaata ctaaagatat agataaatat atgaggtaac
     1741 agctagaga
//
LOCUS       DAEPIN010000187         1615 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.192, whole genome shotgun sequence.
ACCESSION   DAEPIN010000187 DAEPIN010000000
VERSION     DAEPIN010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1615
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.192"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            complement(94..660)
                     /locus_tag="KR250_003832"
     CDS             complement(94..660)
                     /locus_tag="KR250_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBH0069689.1"
                     /translation="MDTKKVTFSSMCIVINIVLGMVISMINIPLLFLDTIGTVLGAVV
                     LGPFWSALIGGCTNLVLGVISGPTNIPFALVNIVLGLIVGYISKKKGFGYKEAIITGV
                     ILSIVCPLIGTPISVLLFGGLSGSGADLLVGFLVQSGQKIFTAAFFPRILSNIVDKPL
                     SCIMVVYFISKMPKGFIKQYSKNTSSSY"
     gene            826..1347
                     /locus_tag="KR250_003833"
     CDS             826..1347
                     /locus_tag="KR250_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HBH0069690.1"
                     /translation="MKRNREIVVVCHCILNCNSKVEGLSEFKGAQNIAIDLINKGYGI
                     IQLPCPEMIMYGINRWGHVKEQFDNLFFREQCRQMLIPHVRQFDEYLKNDYDIKSIIA
                     IDGSPSCGYNKTCSSKRWFGELSGCNELMDKINDIQMVDGKGIFIEELEKLLKEYNLN
                     IPIVGFDELREDK"
BASE COUNT          683 a          185 c          242 g          505 t
ORIGIN      
        1 atactatcta attaaattat taataaaaat gttatataaa aatgagtata tagcatttta
       61 aagagtaata aatcttacat aataaatgat ttattaatat gaactagaag tatttttact
      121 atattgtttt ataaaacctt taggcatttt acttataaaa tataccacca taatacaaga
      181 caaaggtttg tctactatat tactaagaat ccttggaaag aacgcagcag taaatatctt
      241 ctgaccagac tgaactaaaa aaccaaccaa taaatcagca ccacttccac ttaaaccacc
      301 aaacaataaa actgaaatag gagtaccaat taaaggacaa acaatagata aaattactcc
      361 tgtaataata gcttctttat aaccaaaacc ttttttctta gaaatatatc caacaataag
      421 accaagaaca atatttacaa gggcaaaagg tatatttgta ggacctgata taacacctaa
      481 aactaagttt gtacacccac caataagagc actccaaaaa ggacctaata cgacagcccc
      541 aagtacagta ccaattgtat ctaaaaataa taatggtata tttatcatag atataaccat
      601 acctaagaca atattaataa ctatgcacat tgaagaaaat gtgacttttt tagtatccaa
      661 ttataatacc tcctaaaaaa atatatacat ttatcatagt atagattata aaagattact
      721 aattgtatat ttctatatag aatattggaa atatagaaca aataaataat ataattatca
      781 aaatatgtag tatcattaaa attaagaata actaaggaga ttattatgaa gagaaataga
      841 gagattgtag tagtatgtca ttgtatatta aattgtaatt ctaaagtaga gggattgtca
      901 gaatttaagg gagcacaaaa tatagcgata gatttaataa ataaagggta tggaataatt
      961 caactaccat gcccagaaat gattatgtat ggaataaata ggtggggaca tgtaaaagaa
     1021 cagtttgaca atttgttttt tagagaacaa tgtaggcaaa tgttaatacc tcatgtaagg
     1081 cagtttgatg aatatttaaa aaatgattat gatataaaaa gtatcattgc aatagatggc
     1141 agtccaagtt gtggttataa taagacatgt agttctaaac gttggtttgg tgagttaagt
     1201 ggttgcaatg aattgatgga taaaataaat gatatacaaa tggtggatgg gaaaggtatt
     1261 tttatagaag agctagaaaa gctacttaaa gaatataatt taaatatacc tatagttgga
     1321 tttgacgagc ttagagaaga taagtagaga tataaatatt tattgtctca ttatgaaaca
     1381 ccattatatt tggcagaaaa atatgactga tggacagtac ctgaatcatt tttaagtgga
     1441 gtaattagta cttataaaga tgtatccact agttgaaaga aaatgtagag aggtaataaa
     1501 tgaattaaga agtagataat atatataaag gagtgtctaa aagtgaactt tttagtttat
     1561 gagacactct ttttttatat taaatcaagt atattaaatc aaatatattt taatc
//
LOCUS       DAEPIN010000188         1511 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.193, whole genome shotgun sequence.
ACCESSION   DAEPIN010000188 DAEPIN010000000
VERSION     DAEPIN010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1511
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.193"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            387..1103
                     /locus_tag="KR250_003834"
     CDS             387..1103
                     /locus_tag="KR250_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0069691.1"
                     /translation="MNQNILVVDDDREIVDAIEFYLRPDNFNVLKAYDGLEALDILIE
                     NDIKLIIMDVMMPNLDGLKTTLKIREKRNIPIILLSAKSEDMDKIIGLNMGADDYITK
                     PFNPLELTARVKSQLRRYINLGDFSNTNNSNDTNYILKSGGLELNTDTKIVSLDGEEI
                     KMTPLEYKILTLLMSRKGKIFSSHEIYENVWNEDAYSCERTIAVHIRRIREKIEINPK
                     EPKYLKVVWGIGYKIEKLNY"
BASE COUNT          582 a          173 c          204 g          552 t
ORIGIN      
        1 aacctataag ccttattaaa tgctttatac atacctacat taaactacct gtttatatta
       61 aactacatta aaataaatat ttgttataaa ttcttaataa ttatgaaact catttgttat
      121 ttatatttgt taatatatgt ttaaacctaa aaaattaata aaccaaaata acataagaag
      181 tgtgatagat taccctagat attttcataa acttttacct tcagattaat atgggcgtct
      241 atacttttaa atttactata tgcattgaca atatattaaa agtactagtt tttatttcat
      301 aatgaacggg cttactgttt tctaatacct ctttttggta taaaataaaa attggttaaa
      361 aataaaattt caagggggtt aataaattga atcaaaatat attggttgta gatgatgata
      421 gagagatagt tgatgctata gagttctatt tgagacctga taactttaat gttttaaagg
      481 catatgatgg acttgaagca ttggacatac taatcgaaaa tgatattaaa ttaataataa
      541 tggatgtaat gatgcccaat ttagatgggc ttaaaactac acttaaaata agagaaaaaa
      601 ggaatatacc aataattctc ttatctgcaa aatcagaaga tatggacaaa attataggtc
      661 ttaatatggg tgctgatgac tatataacta aaccatttaa tccactagaa cttactgctc
      721 gagtaaaatc tcaacttaga aggtatataa atttaggtga ttttagtaat acaaataact
      781 caaatgatac taattatatt ctaaaaagtg gtggcctaga gctaaataca gatacaaaaa
      841 tagtttcttt agatggtgag gaaattaaaa tgacaccact tgaatataaa atacttacac
      901 ttcttatgtc acgaaaaggt aaaattttct cttctcatga gatttatgaa aatgtctgga
      961 atgaggatgc ttatagttgt gagagaacta ttgctgttca tattagaaga attagagaaa
     1021 agattgaaat taatccaaaa gaaccaaaat atttaaaggt ggtgtggggc attggttaca
     1081 aaattgaaaa acttaattat taatgattct aaagcaaaat ttattttatt attattactg
     1141 tttctattag tttctatatt atttgaagca gtttatgatt tgaataataa cttttctaat
     1201 attaattatt attatagaga gtttgctctc ccttattttt attttttcgg tactcctgta
     1261 catttgtcaa aagtagaatt agagtttgtc tgcataggta ttatattatc aagtttttta
     1321 ttgactatac tatgttttac attgtataaa aaaaataaac aaactaaagt caaattctta
     1381 gagaatgtca taaacctttt ttctaaggta aaaatagaac ttacacttat attaactttg
     1441 atactattaa ccttaaggtt tcatgcattt acaagtaata aaatgtttta caacactaat
     1501 ttaataatct a
//
LOCUS       DAEPIN010000189         1349 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.194, whole genome shotgun sequence.
ACCESSION   DAEPIN010000189 DAEPIN010000000
VERSION     DAEPIN010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1349
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.194"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            <1..>1349
                     /locus_tag="KR250_003835"
     rRNA            <1..>1349
                     /locus_tag="KR250_003835"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          357 a          292 c          408 g          292 t
ORIGIN      
        1 caaggtttcc aggggaaggt tcgtccgccc tgggttagtc gggacctaag ctgaggccga
       61 aaggcgtagg tgatggacaa caggttgata ttcctgtact accgataacc gtttgagaga
      121 agggatgaca cagtaggata agctaagcac actgttggtt atgtgtgccc aagcattgag
      181 gcagtcaaag taggcaaatc cgctttgata atgctgggat gtgatgggga gcgaaattta
      241 gtagcgaagt agctgatttc acactgtcaa gaaaagtctc tatcgaggtt aaaggtaccc
      301 gtaccgcaaa ccgacacagg tgggtgagga gagtatccta aggccagcga gagaactgtt
      361 gttaaggaac tcggcaaaat gaccccgtaa cttagggata aggggtgcca ccatcaggtg
      421 gccgcagaga ataggcccaa gcgactgttt accaaaaaca taggtttctg ctaagtcgca
      481 agacgatgta taggagctga cgcctgcccg gtgctggaag gttaagggga tctgttagag
      541 caatcgaagc agtgaactta agccccagta aacggcggcc gtaactataa cggtcctaag
      601 gtagcgaaat tccttgtcgg gtaagttccg acccgcacga aaggcgtaac gatttgggca
      661 ctgtctcaac aacagactcg gtgaaattgt aattccggtg aagatgccgg atacctgcga
      721 caggacggaa agaccccatg gagctttact gtagcttgac attgggtctt ggtactacat
      781 gtacaggata ggtgggaggc tttgaaacca ggacgccagt tttggcggag ccatccttgg
      841 gataccaccc ttgtagtact gggactctaa ccataggcca tgaatctggt cttgggacac
      901 tgtcaggtgg gcagtttgac tggggcggtc gcctcccaaa aggtaacgga ggcgctcaaa
      961 ggttctctca gtacggtcgg aaatcgtacg tagagtgtaa aggcaaaaga gagcttgatt
     1021 gcaagacata caggtcgagc aaggatgaaa atcggactta gtgatccggt ggttctgcgt
     1081 ggaagggcca tcgctcaacg gataaaagct accctgggga taacaggctt atctccccca
     1141 agagtccaca tcgacgggga ggtttggcac ctcgatgtcg gctcatcaca tcctggggct
     1201 gtagtaggtc ccaagggttg ggctgttcgc ccattaaagt ggtacgcgag ctgggttcag
     1261 aacgtcgtga gacagttcgg tccctatccg tcgcaggcgt aggaaatttg agaagacctg
     1321 tccttagtac gagaggaccg ggatggacg
//
LOCUS       DAEPIN010000190         1163 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.195, whole genome shotgun sequence.
ACCESSION   DAEPIN010000190 DAEPIN010000000
VERSION     DAEPIN010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1163
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.195"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            279..>1163
                     /locus_tag="KR250_003836"
     CDS             279..>1163
                     /locus_tag="KR250_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HBH0069692.1"
                     /translation="MYILNLNTRETIEDFRDKFYVAENSYLILSAPKNLKLLKETLDI
                     DEITFNDCLKFDEITKLDLFDNYDFLSLNTFELRDGEAVIEEVNMYLSDNFILVVVNE
                     EHFLFEFVKNIILKNSQLEKNPVINLFKINYLILREVIKNGFESLEKVEELILQIEDE
                     MMDNINKNHVSRISDVRGLTRIIVKNTRPLLYIGDRIVKENIRYLKYSNVKKYNLENF
                     QGIDFGIDKLYNFALSTRELADKLLDIYSSRVGEKTNNLITKLTLLTAISAPLTIITG
                     IYGMNFRYMPELNWIYGYP"
BASE COUNT          475 a           96 c          171 g          421 t
ORIGIN      
        1 gtgtatgtgc ttgttgtcag tttagcacat gcattttatt ttttagcttt tttagtatca
       61 ataatttttt agtatcaata atatgaaata tgttttaaaa taagtatata gtgtaatctg
      121 taataaaaac tttggttaaa taataaatgt atttaacact taccttataa taaatttaat
      181 ataaatatta aataataatt attaaaatag tgtaaaataa ttacagtaag gattataaaa
      241 aatttttaga atgtaaattg aaatttagga gtagatgtat gtatatactg aatttaaata
      301 caagagagac tattgaagat tttagagata aattttatgt agcagaaaat tcttatctta
      361 tattaagtgc tccaaaaaat ttaaaattgc ttaaggaaac tttggatata gatgaaataa
      421 cttttaatga ttgtcttaaa tttgatgaaa taaccaaact tgatttattt gataattacg
      481 attttttaag cctaaataca tttgaactaa gagatggaga agctgttata gaagaggtaa
      541 atatgtattt atcagataat tttatattgg ttgtagtgaa tgaagaacac tttttatttg
      601 aatttgtaaa aaatattatt ttgaagaact ctcaacttga aaaaaatcca gttattaatc
      661 tatttaagat aaactacttg attcttagag aagttataaa aaatggtttt gagtctttgg
      721 aaaaagttga agagttaatt cttcaaatag aagatgaaat gatggacaac attaataaga
      781 atcatgtatc tagaataagt gatgtaagag gattaacaag aattatagtt aagaatacta
      841 gacctctttt gtatattggg gatagaatag ttaaggaaaa tataagatat ttaaaatatt
      901 caaatgtaaa aaaatataat ttagaaaatt ttcaaggtat agattttgga attgacaaat
      961 tatacaattt cgcattgtca acacgagagt tagcagataa attattagat atttattctt
     1021 ccagagtagg tgagaaaaca aataacttaa ttacaaaatt aaccttgtta acagccatat
     1081 cagcaccact tacaattata actggtatat atggaatgaa ctttagatat atgccagaat
     1141 taaactggat ttatggatat cca
//
LOCUS       DAEPIN010000191         1146 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.196, whole genome shotgun sequence.
ACCESSION   DAEPIN010000191 DAEPIN010000000
VERSION     DAEPIN010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1146
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.196"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            complement(119..640)
                     /locus_tag="KR250_003837"
     CDS             complement(119..640)
                     /locus_tag="KR250_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003374619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBH0069693.1"
                     /translation="MVLLIVDAQKLITNERLYKFNEFVVNVENLIDTARKNNIEVIYV
                     RHDDGVENELTKGKNGFEIYEKFKPCNGEKIFDKKVNSAFKETGLLEYLTNNGEKDII
                     IAGLQTDYCIDATIKCGFEHGFNIIVPAYSNTTVDNFFMSAEQSYKYYNEFIWNERYA
                     KCISLEETLRKMK"
     gene            complement(702..>1146)
                     /locus_tag="KR250_003838"
     CDS             complement(702..>1146)
                     /locus_tag="KR250_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBH0069694.1"
                     /translation="GAFIYLFLKNLFAGSLGEELGWRGFVQNHLQKRHSPLKASITVG
                     FWWGMWHLPIWFTTGFKGLDLIKYIIFFMIAIISISIIMTTFYNLNKTLIIPIAIHQL
                     FNFLIGIINEDLIELINYYAMLYLIFAIALIVINPGNVLYKNIRE"
BASE COUNT          395 a          179 c          100 g          472 t
ORIGIN      
        1 aaataaaatt gatgtgcctt atctataata taatatattt aatctaattt ttaacttgga
       61 atttaacatc cttttttatg aaatcaactt atgtacatat acaattcata ctctattttt
      121 atttcatttt tctaagtgtt tcctctaatg aaatacactt cgcatatctt tcattccata
      181 taaattcatt gtagtattta tatgattgtt ctgctgacat aaaaaaattg tcaacagttg
      241 tatttgaata agctggaact attatattaa atccatgttc aaatccacat tttatagtgg
      301 catcaataca ataatctgtt tggagaccag caataataat atctttttca ccattatttg
      361 ttaggtattc taatagacca gtttctttaa aagcactatt tactttttta tcaaatattt
      421 tttctccatt acatggttta aatttttcgt atatttcaaa gccattttta cctttagtca
      481 attcattttc aactccatca tcatgacgta catatattac ttctatatta ttttttctag
      541 ctgtatcaat taaattttct acatttacta caaattcatt aaatttatat aacctctcgt
      601 ttgttattaa cttttgtgca tcaacaatca gtaaaaccat gtttattcct ccttggatgt
      661 tcttctaata atatctttat atcttaccat aaagatatat attactctct aatattttta
      721 tataatacat ttcctggatt tataactatt aatgcaattg caaatattaa atataacatt
      781 gcatagtaat ttataagctc tattaaatct tcattaatta tccctattaa aaaattaaat
      841 agttgatgta ttgcaatagg tattatcaaa gttttattta gattataaaa agttgtcatt
      901 ataatactga ttgatataat tgcaatcata aaaaatataa tatattttat taaatcaagt
      961 cctttgaatc ctgttgtaaa ccatattggt aaatgccaca tcccccacca aaaacctact
     1021 gttatggatg cttttaaagg agaatgtctt ttctgaagat gattttgaac aaatcctctc
     1081 caacctaatt cctcacctaa tgatcctgca aataaatttt ttaaaaataa atatataaat
     1141 gctccc
//
LOCUS       DAEPIN010000192         1112 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.197, whole genome shotgun sequence.
ACCESSION   DAEPIN010000192 DAEPIN010000000
VERSION     DAEPIN010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1112
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.197"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            350..844
                     /locus_tag="KR250_003839"
     CDS             350..844
                     /locus_tag="KR250_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBH0069695.1"
                     /translation="MKNKKGFTLVELLVVIAIIGILAIVALPALFKNIEKAKIAKLEA
                     DISAIKSASLSYYADESKYTDGGMISWVKKDGKIIINGGFKDDPLADKIENLGMPYNG
                     SYLLMSSPGHEKYLELSILPEGEISKSGLDKLKSDYGSSIDIMNEQNKIDIVIKFLND
                     KSNT"
BASE COUNT          455 a          105 c          158 g          394 t
ORIGIN      
        1 ttatactaga gcataaaaaa tatgtagtat aatcttgttt atatcaagtt atggtattgg
       61 taagtaagta taattaaata gacattattt tctgagtcct ctttttattt tcatctgctt
      121 ttaaataata ttattgacat tgatatgact gtagttttct ttattttaat ccatttctta
      181 tatcttttct aacaagaaat gtcattttaa atcatttcca taaattgtaa aataatagta
      241 tatttgtcat agaaagtgat ttttttgtct atatatggta aaatatagaa agtagaaatc
      301 aacaaaataa aaatttatat attattaatt ttatatggag gggaaataaa tgaaaaataa
      361 aaaaggattt actctagtgg aattattagt agtaattgct ataataggaa tattggcaat
      421 agtagcactt ccagcattat ttaaaaatat agaaaaagca aagatagcta aacttgaagc
      481 tgatataagt gcaataaaaa gtgcgtctct tagctactat gcagatgaat caaaatatac
      541 tgatggagga atgatatcat gggtaaagaa agatggaaaa ataataataa atggtggctt
      601 taaagatgac ccattagcag ataaaataga aaatttaggt atgccttata atggttcata
      661 tctattaatg tcatctcctg gtcatgaaaa atatctagaa ttaagtatac ttccagaagg
      721 agaaataagc aaaagtggtc tagataaatt aaaaagtgat tatggaagtt caatagacat
      781 aatgaacgag caaaacaaaa tagatattgt aataaaattt ttaaatgata aatcgaatac
      841 ttaaatacaa taaattttat ttaataattg ctgatgaaat aaacaatctt gtttggagaa
      901 atgaaaaaag cactcctcaa gaatagaatg cttagttata attacagaag tgtattggca
      961 tacacttcta ttttttatta tatcacataa tttaaaaata cctattcaag ttgtttttaa
     1021 attatgttga gtaatattct tgatagttta tacattaatg aatttcaaaa aaataagtac
     1081 tctttataat aaaaagtact ttaatgtatg tt
//
LOCUS       DAEPIN010000193         1057 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.199, whole genome shotgun sequence.
ACCESSION   DAEPIN010000193 DAEPIN010000000
VERSION     DAEPIN010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1057
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.199"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            58..813
                     /locus_tag="KR250_003840"
     CDS             58..813
                     /locus_tag="KR250_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1835 domain-containing protein"
                     /protein_id="HBH0069696.1"
                     /translation="MAHICFEDICYLLLKEAMKLKLIPHNKIVLFEDDLSIGNIFDVN
                     NYELRENIFKIRNVFENNPKLLFKPDLLEDRKLSKCRNIIIWCANNPKDLCGVYYVVS
                     KFQDKNIKIALVDEKKTEDALIRYSFTSEMSPEDFPFFLDKTLEIGFNEKLEFKNKWD
                     VLVKENSIFRALVGNEIKSVEETYFDKYILRNISDKWQPLIEVVANCMFEDEMRVKDW
                     FILWRLEKMASLKIVKIKGSKGDFYSDKEIRKV"
BASE COUNT          449 a           71 c          171 g          366 t
ORIGIN      
        1 attacagttt atatattata ataaaataaa ttgttaattt taaaagaggt gaattttatg
       61 gcacatatat gctttgagga tatttgttat ttgttattaa aagaagcaat gaagttaaag
      121 ttaataccac ataataaaat tgtattattt gaagatgatt tatcaatagg taatattttt
      181 gatgtaaata actacgaatt aagagaaaat atttttaaga ttagaaatgt ttttgaaaat
      241 aatccgaaac tattattcaa gccagatttg ttagaagata ggaaattatc aaaatgtaga
      301 aatataataa tatggtgtgc aaataaccca aaagatttgt gtggagtata ttatgtagtg
      361 agtaagtttc aagataaaaa tataaagatt gcattagtag atgaaaaaaa gacagaagat
      421 gctctaatta gatattcatt taccagtgaa atgtctccag aagattttcc attcttttta
      481 gataagacac tagaaattgg ctttaatgaa aaactagaat ttaaaaataa atgggatgtg
      541 ttagttaaag aaaattctat ttttagagca ttagtaggta acgaaattaa aagtgttgaa
      601 gaaacttatt ttgataaata tatattaaga aatatcagtg acaaatggca acctttaatt
      661 gaagtggttg caaactgtat gtttgaagat gaaatgcgtg taaaagattg gtttatactt
      721 tggagattag agaagatggc aagtttaaaa atagtaaaaa taaaaggaag taaaggggat
      781 ttttattctg ataaggagat aagaaaagtt taaaaattga atttataata aattgtatta
      841 aataatgggt ataactatga agaaaaatat cttaacagta ttaatattat aattagcaat
      901 aagtataata tttattctat aatttggaaa tataataact aataccttac gaataagtgt
      961 gctaaataaa aaagaaatac ttatttcagg gatattagta tatattttaa ctacattttt
     1021 acttaatagt attaaaatat ggtgctatag agtaaat
//
LOCUS       DAEPIN010000194         1012 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.200, whole genome shotgun sequence.
ACCESSION   DAEPIN010000194 DAEPIN010000000
VERSION     DAEPIN010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1012
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.200"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            <1..518
                     /locus_tag="KR250_003841"
     CDS             <1..518
                     /locus_tag="KR250_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HBH0069697.1"
                     /translation="KNLEQLNNPTHSTNRIYDAVGIARCIRSQAGGGGAKTGLYFIDL
                     NKNSKVTINARCLKAKYNAGVTNRNCDNSGVLVNAVLTPDRVNKRQNGRRIKESGETM
                     FTLTAQDKHGILKNGDIRRLTPKECFRLQGFPDKYYERAASVCSDSQLYKQAGNAVTA
                     NVVYEIAKRMG"
     gene            598..807
                     /locus_tag="KR250_003842"
     CDS             598..807
                     /locus_tag="KR250_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069698.1"
                     /translation="MYEYILRWQIGLSLENRKIHYTYGSKEALRKKAKALAKDENIVL
                     ITIDKVDEVIKNTISEKIIERFENL"
     gene            819..>1012
                     /locus_tag="KR250_003843"
     CDS             819..>1012
                     /locus_tag="KR250_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HBH0069699.1"
                     /translation="MIIHKFIIHVLDKNSDTPILNDFEGRVNQDMVLFFQKKISKVSR
                     DNDIRTAVFNNYSNNLIKKC"
BASE COUNT          430 a          100 c          204 g          278 t
ORIGIN      
        1 gaaaaaatct tgagcaacta aataatccaa ctcatagtac aaatagaatt tatgatgcag
       61 ttggaattgc tagatgtatt agaagtcagg caggaggtgg aggtgctaaa acaggtctat
      121 actttataga cttaaataaa aactctaaag taacaataaa tgctagatgc cttaaagcaa
      181 aatataatgc aggtgtgaca aatagaaatt gtgataatag tggagtttta gttaatgcag
      241 ttttaacgcc cgatagggta aataaaagac aaaatggtcg tagaattaaa gaaagcggag
      301 aaacaatgtt cacattgaca gctcaagata aacatggaat tttgaaaaat ggagatataa
      361 gaaggttaac accaaaggaa tgctttaggt tgcaaggatt tccggataaa tattacgaaa
      421 gagcagcaag tgtatgctca gatagtcaac tgtacaagca agcaggaaat gctgttactg
      481 caaatgttgt atatgaaata gcaaaaagaa tgggctaaaa gttgcaaaat gtcttttagt
      541 atgaatattt ttgaagtgtt ttgtaactct caaaaatgaa aataaggggt gggataaatg
      601 tatgaatata tattaagatg gcaaatagga ttatcgttag aaaatagaaa aatacattat
      661 acatatggaa gtaaagaagc tttaagaaag aaagcaaagg cattggctaa agatgaaaat
      721 atagtactaa taactataga taaggtagat gaagttataa aaaatactat aagcgagaag
      781 attatagaac gttttgaaaa tttataaggg gtggaattat gataatacac aaatttataa
      841 tacatgtttt agataagaat agtgatacac caatattgaa tgattttgaa ggtagggtta
      901 atcaagatat ggtcctattt tttcaaaaga aaataagcaa agtatcaaga gataatgaca
      961 tcagaacagc agtatttaat aactatagta acaatctaat taagaagtgt tg
//
LOCUS       DAEPIN010000195         1008 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.201, whole genome shotgun sequence.
ACCESSION   DAEPIN010000195 DAEPIN010000000
VERSION     DAEPIN010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1008
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.201"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            complement(<1..194)
                     /locus_tag="KR250_003844"
     CDS             complement(<1..194)
                     /locus_tag="KR250_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HBH0069700.1"
                     /translation="MIIHKFIIHVLDKNSDTPILNDFEGRVSQDMDLFFQKKIKTVSR
                     AKDIRTAVFNDYSNNLIKKC"
     gene            complement(191..412)
                     /locus_tag="KR250_003845"
     CDS             complement(191..412)
                     /locus_tag="KR250_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069701.1"
                     /translation="MSKYVLRWQKGLLLDERKINYSCGSKEMLKQKAELLAKDDKILL
                     VTIDKVEEVIKDTRSQKMTEYYCDGGIEI"
     gene            complement(491..>1008)
                     /locus_tag="KR250_003846"
     CDS             complement(491..>1008)
                     /locus_tag="KR250_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HBH0069702.1"
                     /translation="KNLEQLNNPTHSTNRIYDAVGIARCIRSQAGGGGAKTGLYFIDL
                     NKNSKVTINARCLKAKYNAGVTNRNCDNSGVLVNAVLTPDRVNKRQNGRRIKESGEPM
                     FTLTAQDKHGILKNGDIRRLTPKECFRLQGFPDKYYERAASVCSDSQLYKQAGNAVTA
                     NVVYEIAKRMG"
BASE COUNT          276 a          213 c          105 g          414 t
ORIGIN      
        1 caacacttct taattaaatt gttactatag tcattaaata ctgctgttct aatgtcttta
       61 gctcttgata ctgtcttaat cttcttctga aaaaatagat ccatatcttg actaacccta
      121 ccctcaaagt catttagtat tggtgtatcg ctattcttat ctaaaacatg tattataaat
      181 ttgtgtatta tcatatttca attcctccat cacaataata ttcagtcatt ttttgacttc
      241 tagtatcctt tataacttct tcaactttat ctattgttac aagcagtatc ttgtcatctt
      301 tagctaataa ttctgctttt tgttttaaca tttccttact tccacaagaa taatttatct
      361 tacgttcatc caacaataac cctttttgcc atctaagcac atactttgac atttattcac
      421 cccttatttt catttttgag agttacaaaa cacttcaaca atactcatac taaaagacat
      481 tttgcaactc ttagcccatt ctttttgcta tttcatatac aacatttgca gtaacagcat
      541 ttcccgcttg tttgtacagt tgactatctg agcatacact tgctgctctt tcgtaatatt
      601 tatccggaaa tccttgcaac ctaaagcatt cctttggtgt taaccttctt atatctccat
      661 ttttcaaaat tccatgttta tcttgagctg ttagtgtgaa cattggttct ccgctttctt
      721 taattctacg accattttgt cttttattta ccctatcggg cgttaaaact gcattaacta
      781 aaactccact attatcacaa tttctatttg tcacacctgc attatatttt gctttaaggc
      841 atctagcatt tattgttact ttagagtttt tatttaagtc tataaagtat agacctgttt
      901 tagcacctcc acctcctgcc tgacttctaa tacatctagc aattccaact gcatcataaa
      961 ttctatttgt actatgagtt ggattattta gttgctcaag attttttc
//
LOCUS       DAEPIN010000196          997 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.202, whole genome shotgun sequence.
ACCESSION   DAEPIN010000196 DAEPIN010000000
VERSION     DAEPIN010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..997
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.202"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            <1..552
                     /locus_tag="KR250_003847"
     CDS             <1..552
                     /locus_tag="KR250_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069703.1"
                     /translation="TPVKADNSYIKQQIDSLNKWVGDAPEKIKLDRMGYKDIGAEFQK
                     GNALGTKWQNAITDKFKDTFDINKMLEDAKKKLGLDDLWNKQNPLNNLGGFGGDLGKN
                     VKDTAGNTAKMAKTMDKSQEDLKYLRDIAEQETINRFTGVNIKIDMNNTNNISKDTDV
                     DGIVNVLTEKLNDAMIVSAEGIV"
     gene            568..>997
                     /locus_tag="KR250_003848"
     CDS             568..>997
                     /locus_tag="KR250_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HBH0069704.1"
                     /translation="MAYDFYLDGVQLPIPPPKLEIKVTNKNKIVDLINTGEVNILKKE
                     GLSEISFEAEFTHNKLPFYRGAFRDVQFFLSKLELLKTDCKPFQFIVSRELGNKVLFN
                     TNIKVSLEEYAISEDAENGSDTKVAIKLKQYRDYSTKKLVL"
BASE COUNT          431 a          113 c          188 g          265 t
ORIGIN      
        1 acgcctgtca aggcagataa tagttacatc aaacaacaga ttgacagttt aaataaatgg
       61 gtaggagatg caccagaaaa aataaaattg gatagaatgg gatataaaga tattggagca
      121 gaatttcaaa aaggaaatgc acttggaact aaatggcaaa atgctataac tgataaattt
      181 aaagatactt ttgacattaa taagatgcta gaagatgcaa agaaaaaatt aggattagac
      241 gatttgtgga ataaacaaaa tcctttaaac aaccttggtg gatttggtgg agatttagga
      301 aaaaatgtaa aagacacagc aggaaacaca gccaaaatgg ctaaaacaat ggataaaagt
      361 caagaagacc ttaaatactt aagagacatt gcagaacaag aaacaataaa cagattcaca
      421 ggagtaaaca taaaaattga tatgaacaat actaacaata taagtaaaga cacggatgtg
      481 gatggtatag taaatgtttt aacagaaaaa ctgaatgatg ctatgattgt atcagcagaa
      541 ggaatagttt agaaaggagg gatataaatg gcttatgact tttatttaga tggagtacaa
      601 ttaccaatac ctccgccaaa gttagagatt aaagttacaa ataaaaacaa gatagttgat
      661 ttgataaata ctggagaagt aaacatacta aaaaaagaag gattatctga aataagtttt
      721 gaagcagaat ttacacataa taagttgcca ttctatcgtg gagcttttag ggatgttcaa
      781 ttctttttaa gtaaactgga actattaaaa actgattgta agccatttca atttattgta
      841 tcgagggaat taggtaataa agtactattt aacactaata taaaagtatc tcttgaagag
      901 tatgctattt cagaagatgc agaaaatggt tcagatacaa aagttgcaat aaagttaaag
      961 caatatagag attactcaac taaaaagtta gttcttg
//
LOCUS       DAEPIN010000197          903 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.204, whole genome shotgun sequence.
ACCESSION   DAEPIN010000197 DAEPIN010000000
VERSION     DAEPIN010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..903
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.204"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            complement(<1..424)
                     /locus_tag="KR250_003849"
     CDS             complement(<1..424)
                     /locus_tag="KR250_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HBH0069705.1"
                     /translation="MNVTIVFLATNIFIKLVILAIAFDTLLGCLRAIKTHQFNSSFGI
                     NGGIRKVAMIACIFFLAVVDILTKFNFLFMLPQDWVDFLRLNHLGISEFFSILFILYE
                     SVSILKNMYLCGLPVPKRLKEKIGSLLDTMTDELNVKGG"
     gene            complement(486..623)
                     /locus_tag="KR250_003850"
     CDS             complement(486..623)
                     /locus_tag="KR250_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HBH0069706.1"
                     /translation="MNWYKIITDFYNNGNWTKEQVKTAVTKNKITASEYKEIVREDYI
                     A"
     gene            complement(625..>903)
                     /locus_tag="KR250_003851"
     CDS             complement(625..>903)
                     /locus_tag="KR250_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069707.1"
                     /translation="LDTEEQNIKYVGMIHYEPNLLSEENLKQGILVEELPTPKYTENK
                     ETKLFINIDTKEVFYRYTDIKSSIEDKVNSTEQTIADLTFQLMSNGVI"
BASE COUNT          288 a          153 c           88 g          374 t
ORIGIN      
        1 tacctccttt aacatttaat tcatctgtca tagtatctaa taaactacct attttctctt
       61 ttaatcgttt aggaactggt aatccacata agtacatatt ttttaatata cttacacttt
      121 catatagaat aaataaaata gagaaaaatt cagatattcc aagatgattt aatcgcaaaa
      181 aatcaaccca atcttgtggc aacataaata aaaagttaaa ctttgtaaga atgtcaacta
      241 ctgctagaaa aaatatacat gctatcattg ctacttttct tattcctcca tttattccaa
      301 aagagctatt aaactgatgt gtttttattg ctcttaagca acctaacagt gtatcaaatg
      361 ctattgctaa tattactaat tttataaata tatttgttgc taaaaaaact attgttacgt
      421 tcatatttcc tccttattct gcattaaaat aagacttaga aattatctaa gtctatttaa
      481 aagaactacg ctatatagtc ctctcttaca atttctttat attcacttgc tgttatctta
      541 ttctttgtaa ccgctgtttt aacttgctct ttagtccaat taccattatt atagaaatct
      601 gttattatct tataccaatt catactatat caccccatta ctcattaatt gaaatgttaa
      661 atctgctatt gtttgttctg tagaatttac tttatcttct atgctacttt taatatctgt
      721 atatctatag aaaacctctt tagtatctat atttataaat aactttgttt ctttattttc
      781 tgtgtatttt ggtgttggta attcctcgac caaaattcct tgttttaagt tttcctccga
      841 taacaaattt ggttcatagt gtatcatacc aacatatttt atattttgtt cctctgtgtc
      901 tag
//
LOCUS       DAEPIN010000198          886 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.206, whole genome shotgun sequence.
ACCESSION   DAEPIN010000198 DAEPIN010000000
VERSION     DAEPIN010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..886
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.206"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            complement(303..680)
                     /locus_tag="KR250_003852"
     CDS             complement(303..680)
                     /locus_tag="KR250_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBH0069708.1"
                     /translation="MTITKIPPAELKVMKFIWSSDFTVASKDVIEAMEKLYNWKQTTT
                     LTLLSRLVNKQFLSARKIDRHTHYTVLIGEEEYLHFETKTFLDNMHGSSIESFMKSLF
                     KKGVNEEELNSVEKWVKDIKKDE"
BASE COUNT          347 a          129 c           63 g          347 t
ORIGIN      
        1 taatcaaaac aaaaataaaa ttatgccctt tatatctaaa acaattaatt tacatgatta
       61 atatacaaaa attctgactt ttaaattcgt atacaatatc aaactaaaat aatattaatg
      121 acaaacccaa tataaattaa attcaaacag gtttgatttt acatcaaact tattgtatta
      181 atattaaata acaagacaaa aatatttata aaaatcacaa attttacata aaactttata
      241 tttgattcat ttaactaatt attgtaaaaa ctatttattt tatgataatt aactacacca
      301 aattattcat cttttttaat atctttaacc catttttcaa cagagttcaa ttcttcttca
      361 tttaccccct ttttgaacaa ggatttcata aaactctcaa tagaacttcc atgcatatta
      421 tctaaaaaag tttttgtttc aaaatgtaaa tactcttctt ctccaataag aactgtataa
      481 tgtgtatgtc tatcaatttt tcttgcactt aagaattgct tatttactaa tcttgataaa
      541 agtgttagtg ttgtagtttg cttccaatta tatagttttt ccatagcttc aatgacatct
      601 tttgatgcta ctgtaaaatc tgaactccaa ataaatttca tgacttttaa ttctgctggt
      661 ggtatcttag taatagtcat atgattttac actcctctca aacttctaca taattaaata
      721 ctatattaat agacttttcg agtcaaaggt tttatttaaa atatctcatc caaatttatt
      781 aatacaaaaa atttatattt ttattcaaaa ttaaacacac aatctattaa tataaagata
      841 aatatctatt ctataagtat aacagattat caaattaaaa ttctaa
//
LOCUS       DAEPIN010000199          849 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.208, whole genome shotgun sequence.
ACCESSION   DAEPIN010000199 DAEPIN010000000
VERSION     DAEPIN010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..849
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.208"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            <1..>849
                     /locus_tag="KR250_003853"
     CDS             <1..>849
                     /locus_tag="KR250_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBH0069709.1"
                     /translation="RIVCDVPPNALNKVFMNLGREIKKFSDYKENYSNILRYIFINKK
                     SSQRFPTDSEFSNAFETKDIYNTKSKNKLYILERLENYNNKEIVDLENLINNNMLNIE
                     HIMPQTLTNKWKESLGDNYKEIHDKYLHTIGNLTLTGYNSKLSNKTFEEKKEMEDGFK
                     DSRLYLNKYISSIDKWNEEEIKNRTKILLDRAVEIWKYPSVTYEPPSNSENIFTLEDD
                     DVNFTNESIVSFSILGESYKAKNWTEFYEQVALTLYDLDSVRFNSIVDKTYKRDFIDN
                     IKSRDA"
BASE COUNT          375 a           72 c          135 g          267 t
ORIGIN      
        1 aagaatagta tgtgatgtac ctcctaatgc actgaataaa gtatttatga acttaggaag
       61 ggaaattaaa aaatttagtg attataaaga aaactattct aatatattaa gatatatttt
      121 tataaataag aaatcatctc aaagatttcc tactgacagt gaattttcaa atgcttttga
      181 aactaaagat atatataata ctaagtcaaa gaataagtta tatatacttg agaggttaga
      241 gaattataat aataaagaaa ttgttgattt agaaaattta atcaataata atatgctaaa
      301 tatagagcat ataatgccac agacattaac aaataaatgg aaggaatcac taggagataa
      361 ctataaggag atacatgata aatatttaca tacaattgga aatttaactt tgacaggata
      421 taattcaaaa ctaagtaata aaacatttga agaaaagaaa gaaatggaag atggatttaa
      481 agatagtagg ctttatctaa ataaatatat ttcatctata gataaatgga atgaagaaga
      541 aataaaaaat agaactaaga tattactaga tagagctgta gaaatttgga aatatccaag
      601 tgttacatat gaacctccaa gtaacagtga aaatatattt acgttagagg atgatgatgt
      661 taattttaca aatgaaagta tagtatcatt tagtatatta ggagagtcat ataaggctaa
      721 aaattggaca gaattttatg aacaagttgc tcttacttta tatgatttgg attctgtaag
      781 gtttaacagt attgtagaca agacttataa aagagatttt atagataata ttaagtctag
      841 agatgcata
//
LOCUS       DAEPIN010000200          823 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.209, whole genome shotgun sequence.
ACCESSION   DAEPIN010000200 DAEPIN010000000
VERSION     DAEPIN010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..823
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.209"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            complement(62..553)
                     /locus_tag="KR250_003854"
     CDS             complement(62..553)
                     /locus_tag="KR250_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HBH0069710.1"
                     /translation="MGMIGCYTKISEENVFKLQQAEENLQDFVFEDANENSTINIDKA
                     WHAIHFTLTGCPFGGDDDNIFSKLVLSGNILMEIDGEFPVMLITANDVKKLSKAMNSL
                     EEQAFRKRFNINEMLENNIYPVMNDESEDDFFEYVWANLIELKKFIQEASNDGLAVIF
                     FIM"
BASE COUNT          292 a          133 c           78 g          320 t
ORIGIN      
        1 atcaaaaggc ttcatatcta agatatgtga tgacactaat attcacaatt tttagtaatt
       61 atcacattat aaaaaatatt acagctagtc catcattaga tgcttcttgt ataaactttt
      121 taagctcaat tagatttgcc catacatatt caaaaaaatc atcttcactc tcatcattca
      181 taacaggata aatattattt tctaacatct cattaatatt aaatcttttt ctaaatgctt
      241 gttcttccaa actgttcatt gcttttgata gtttttttac atcatttgct gtaatcaaca
      301 tgacaggaaa ttctccatca atttccataa gtatgtttcc acttagtact aatttgctaa
      361 atatattatc atcatctcca ccaaaaggac aacctgtcaa agtaaagtga atggcatgcc
      421 atgctttatc aatattgata gtactatttt cgttggcatc ttcaaaaaca aaatcttgta
      481 aattttcttc tgcttgctgt aatttaaaaa cattctcttc acttatttta gtataacatc
      541 ctatcattcc cataattaca ttcctcctaa gtagcgtttt tttaattgta acttataatt
      601 aaacttagtt acaataaaat atattgagaa aagtattatt tcatcttaaa tttaaatacc
      661 tatattatca actaaaattc tgtgtttata gcgtaagtat tattttttcc taaaaattaa
      721 atgaatcaga ggttctatag gcaatattga ctataaataa gaactattac tttttaaaaa
      781 aaatttaaga aattatactg tagaatcatg ctctacttat tag
//
LOCUS       DAEPIN010000201          736 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.210, whole genome shotgun sequence.
ACCESSION   DAEPIN010000201 DAEPIN010000000
VERSION     DAEPIN010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..736
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.210"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            <1..624
                     /locus_tag="KR250_003855"
     CDS             <1..624
                     /locus_tag="KR250_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HBH0069711.1"
                     /translation="AYYRRMQMRKEQDPEGYKVYGLGEWGETGGAILKNYVIHEFTTE
                     FEYFDNMRLSQDFGFNHANVVLRIGFKDGELYICNEIYVHEMDTSEIIKIANSIGLEK
                     TLFMYCDSAEPDRIKMWKNAGYKAKGVKKGPGSVKAQIDYLKQLRIHVHPSCTNTIKE
                     IQQWKWKQDERTGLYLDEPVEFMDDAMAALRYSIDNKLKNNGISFLK"
BASE COUNT          305 a           83 c          147 g          201 t
ORIGIN      
        1 gcttactaca gaagaatgca aatgagaaaa gagcaagacc cagaagggta caaagtctat
       61 ggtcttggag aatggggaga aactggtgga gcaatactta aaaattatgt tatacatgaa
      121 tttactacag aatttgagta ttttgacaat atgaggttat cacaagactt tggatttaac
      181 catgcaaatg tagtacttag aattggcttt aaggatggag agttatatat atgtaacgaa
      241 atatatgtac atgaaatgga tacctcagaa atcataaaga ttgcaaatag tataggttta
      301 gaaaagactc tatttatgta ctgtgatagt gctgaaccag atagaattaa gatgtggaag
      361 aatgcaggat ataaagctaa aggagttaaa aaagggccag gaagtgttaa agctcaaata
      421 gattatttga aacaattaag aatacatgta catcctagtt gcactaatac cataaaagaa
      481 atacaacaat ggaaatggaa acaagacgaa agaactggat tatatcttga tgaaccagtt
      541 gagtttatgg atgatgcaat ggctgctctt agatattcta tagataataa gcttaaaaat
      601 aatggaataa gcttcttaaa gtaaaggagg tgttaaatat ttatataagt gaaacagatt
      661 taataaaagt tcagttaaaa aaagagagca cctttaacct agtaaaagtc atagaacact
      721 acatcttaaa acatag
//
LOCUS       DAEPIN010000202          736 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.211, whole genome shotgun sequence.
ACCESSION   DAEPIN010000202 DAEPIN010000000
VERSION     DAEPIN010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..736
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.211"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            <1..624
                     /locus_tag="KR250_003856"
     CDS             <1..624
                     /locus_tag="KR250_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HBH0069712.1"
                     /translation="AYYRRMQMRKEQDPEGYKVYGLGEWGETGGAILKNYVIHEFPTE
                     SEYFDNMRLSQDFGFNHANVVLRIGFKDGELYICNEIYAHEMDTSEIIKIANSIGLEK
                     TLFMYCDSAEPDRIKMWKSAGYKAKGVKKGPGSVKAQIDYLKQLRIHVHPSCTNTIKE
                     IQQWKWKQDERTGLYLDEPVEFMDDAMAALRYSIDNKLKNNGISFLK"
BASE COUNT          304 a           85 c          149 g          198 t
ORIGIN      
        1 gcttactaca gaagaatgca aatgagaaaa gagcaagacc cagaagggta caaagtctat
       61 ggtcttggag aatggggaga aactggtgga gcaatactta aaaattatgt tatacatgaa
      121 tttcctacag aatctgagta ttttgacaat atgaggttat cacaagactt tggatttaac
      181 catgcaaatg tagtacttag aattggcttt aaggatggag agttatatat atgtaacgaa
      241 atatatgcac atgaaatgga tacctcagaa atcataaaga ttgcaaatag tataggttta
      301 gaaaagactc tatttatgta ctgtgatagt gctgaaccag atagaattaa gatgtggaag
      361 agtgcaggat ataaagctaa aggagttaaa aaaggaccag gaagtgttaa agctcaaata
      421 gattatttga aacaattaag aatacatgta catcctagtt gcactaatac cataaaagaa
      481 atacaacaat ggaaatggaa acaagatgaa agaactggat tatatcttga tgaaccagtt
      541 gagtttatgg atgatgcaat ggctgcgctt agatattcta tagataataa gcttaaaaat
      601 aatggaataa gcttcttaaa gtaaaggagg tgttaaatat ttatataagt gaaacagatt
      661 taataaaagt tcagttaaaa aaagagagca ccattaacct agtaaaagtc atagaacact
      721 acatcttaaa gcatag
//
LOCUS       DAEPIN010000203          636 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.213, whole genome shotgun sequence.
ACCESSION   DAEPIN010000203 DAEPIN010000000
VERSION     DAEPIN010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..636
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.213"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            complement(<1..634)
                     /locus_tag="KR250_003857"
     rRNA            complement(<1..634)
                     /locus_tag="KR250_003857"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          140 a          175 c          117 g          204 t
ORIGIN      
        1 ctacttaacg taactcgttg gcccgttcta caaaaagtac gcggtcacac aagaaatgtg
       61 cttccacagc ttgtaagtgc agggtttcag gttctatttc actcccctcc cggggttctt
      121 ttcacctttc cctcacggta ctatacgcta tcggtcacta ggtagtattt agccttggag
      181 ggtggtccct cctgcttccc acagggtttc acgtgtcccg tggtactctg gatcatatct
      241 aaagtcttct cgtttaacat acaggactat taccttctgt ggtgggtctt tccaaacctc
      301 ttcaattacg atacctcttc gttatgatat gtccgcaacc ccaataaaga aaactttatt
      361 ggtttgggct aatccgcgtt cgctcgccgc tacttacgga atcgaatttc tttctcttcc
      421 ttcaggtact tagatgtttc agttccctga gttcccctca ttaagctatg tattcactta
      481 atgatactta gacattactc taagtgagtt tcctcattcg gaaatcttcg gatcaaagtt
      541 tacgtgcaac tccccgaagc ttatcgcagc ttatcacgtc cttcatcggc tcctagtgcc
      601 aaggcatccg ccctgcaccc ttaataactt gaccag
//
LOCUS       DAEPIN010000204          611 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.214, whole genome shotgun sequence.
ACCESSION   DAEPIN010000204 DAEPIN010000000
VERSION     DAEPIN010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..611
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.214"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            <1..>611
                     /locus_tag="KR250_003858"
     rRNA            <1..>611
                     /locus_tag="KR250_003858"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          172 a          126 c          179 g          134 t
ORIGIN      
        1 tttaactgag cgttcagtta cttgacgtag acccgaaacc gggcgaccta cccatgagca
       61 ggatgaagcg aaagtaaaat ttcgtggagg tccgaaccca cgagcgttga aaagctcggg
      121 gatgacttgt gggtagcggt gaaattccaa tcgagcccgg agatagctgg ttctccccga
      181 aatagcttta gggctagcct caaggtgaga gatacggagg tagagcactg aatgtcctag
      241 ggggtattgc acctaccgaa gactatcaaa ctccgaatgc cgtcatctta tacttgggag
      301 tcagactgtg ggtgataaga ttcatagtcg aaagggcaac agcccagatc gtcagctaag
      361 gtccctaaat gtaagttaag tggtaaagga tgtgggattg cacagacaac caggatgttg
      421 gcttagaagc agccactcat tcaaagagtg cgtaatagct cactggtcga gtgatcctgc
      481 gccgaagatt tccggggcta aaacttacta ccgaagctac ggcatcagta atgatgggta
      541 ggggagcttc ccatacgggt tgaagcatga ccgtaaggac atgtggacag tatgggagtg
      601 agaatgttgg c
//
LOCUS       DAEPIN010000205          577 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.215, whole genome shotgun sequence.
ACCESSION   DAEPIN010000205 DAEPIN010000000
VERSION     DAEPIN010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..577
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.215"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            <1..>577
                     /locus_tag="KR250_003859"
     rRNA            <1..>577
                     /locus_tag="KR250_003859"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          154 a          121 c          190 g          112 t
ORIGIN      
        1 caggatggac ccgcgtctga ttagctagtt ggtaaggtaa cggcttacca aggcgacgat
       61 cagtagccga cctgagaggg tgatcggcca cattggaact gagacacggt ccaaactcct
      121 acgggaggca gcagtgggga atattgcaca atgggcgaaa gcctgatgca gcaacgccgc
      181 gtgagtgatg aaggccttcg ggtcgtaaaa ctctgtcctc aaggaagata atgacggtac
      241 ttgaggagga agccccggct aactacgtgc cagcagccgc ggtaatacgt agggggctag
      301 cgttatccgg atttactggg cgtaaagggt gcgtaggcgg tctttcaagt caggagtgaa
      361 aggctacggc tcaaccgtag taagctcttg aaactgggag acttgagtgc aggagaggag
      421 agtggaattc ctagtgtagc ggtgaaatgc gtagatatta ggaggaacac cagttgcgaa
      481 ggcggctctc tggactgtaa ctgacgctga ggcacgaaag cgtggggagc aaacaggatt
      541 agataccctg gtagtccacg ctgtaaacga tgagtac
//
LOCUS       DAEPIN010000206          562 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.216, whole genome shotgun sequence.
ACCESSION   DAEPIN010000206 DAEPIN010000000
VERSION     DAEPIN010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..562
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.216"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            286..>562
                     /locus_tag="KR250_003860"
     CDS             286..>562
                     /locus_tag="KR250_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH0069713.1"
                     /translation="MIAVKKAYKFRMYPNKKQQELINKTFGCCRFVYNKYLAKRIDVY
                     KSDKETFTYKQCSSDLTNLKKELKWLKEPDKFSLQNALKDLDNAYKKF"
BASE COUNT          245 a           58 c           83 g          176 t
ORIGIN      
        1 gaaggataag atatagtctg tgcttacatg aaagtgtaag aagttcataa gagaactgca
       61 taggtagtag cgagcttatg tgaacgacaa cctcaaaaat gataaaagtt aagacttacg
      121 gttcttaaat atgttattta aaattaaata taaaaccttc taaataattg ttttaaataa
      181 ttacatttgt taatattatg ataggtggtt aataaattgt tcaccacagc attatataat
      241 atattaaaat cattaaaaag tgtatcaaaa aacaattaaa gtgaggtgat agcagtgaaa
      301 aaagcctata agtttagaat gtatccaaat aaaaaacaac aagagttaat taacaaaact
      361 tttggatgtt gtagatttgt atataataaa tatcttgcta aaagaataga tgtttataaa
      421 agcgataaag aaacttttac ttataaacag tgtagttctg atttgaccaa tttaaaaaaa
      481 gaattaaaat ggcttaagga acctgacaaa ttttcactac aaaacgcctt aaaagattta
      541 gataatgcat ataaaaaatt ct
//
LOCUS       DAEPIN010000207          543 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.219, whole genome shotgun sequence.
ACCESSION   DAEPIN010000207 DAEPIN010000000
VERSION     DAEPIN010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..543
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.219"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            complement(<1..534)
                     /gene="dcm"
                     /locus_tag="KR250_003861"
     CDS             complement(<1..534)
                     /gene="dcm"
                     /locus_tag="KR250_003861"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HBH0069714.1"
                     /translation="MLTFLDLFAGIGGFRLGMEKAGHKCLGHCEYDKFANLSYNAMHK
                     PKEDEWFERDIREIRTENIPRADVWCFGFPCQDISVAGKQFGFRGERSSLFFTVTKLI
                     RELKEEDRPKYLLIENVKNLLSVNGGFDFLKVLVELDEIGYDAEWQVLNSKNFGVPQN
                     RERIFIVGHFRGRSTRKV"
BASE COUNT          159 a          122 c           72 g          190 t
ORIGIN      
        1 tacttttcgt gtacttcgtc ctctaaaatg tccaacaatg aatattcgtt ctctattttg
       61 gggtactccg aagtttttag aattaagaac ttgccactct gcatcatagc cgatttcatc
      121 cagttcaacg agaactttga ggaaatcaaa tcctccatta acactaagta gatttttaac
      181 gttttcaata agtaaatact tgggtctatc ttcttctttg agttctctaa taagttttgt
      241 aactgtaaaa aataaacttg aacgttctcc tctgaatcca aattgtttcc ctgcaacaga
      301 aatgtcttga catgggaatc caaaacacca gacatctgct cttgggatat tttctgttct
      361 aatttctcta atatctcttt caaaccattc atcctccttc ggtttgtgca tggcattata
      421 acttaaattt gcgaatttat catattcgca atgtcccaaa catttatgtc ctgctttttc
      481 catccctagc ctaaagccac ctatccctgc gaataaatct aaaaatgtaa gcaatacaac
      541 gcc
//
LOCUS       DAEPIN010000208          542 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.220, whole genome shotgun sequence.
ACCESSION   DAEPIN010000208 DAEPIN010000000
VERSION     DAEPIN010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..542
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.220"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            65..211
                     /locus_tag="KR250_003862"
     CDS             65..211
                     /locus_tag="KR250_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069715.1"
                     /translation="MVFETFDKTIELYPNAKPIFHSDIGFRLDDESFEEYLGRVFRKR
                     CKGI"
BASE COUNT          249 a           32 c           88 g          173 t
ORIGIN      
        1 ttatttggag caacaaactt attattgttt atagtggact gttggaaaat cgaataataa
       61 taacttggta ttcgaaactt ttgataaaac aatagaacta tatccaaatg caaaacctat
      121 ttttcatagt gatataggat ttagattaga tgatgaaagt tttgaagagt atttaggaag
      181 agtatttaga aagagatgca aagggatata agattgtaaa taaaaatgga ttttttaatg
      241 aatttgattt ttatgaagca gtaaatatat gtttaaatat ctcaatagaa gatgcgataa
      301 aatcatataa tatattagtt aaatcattag taatgttaga taaaagatta ggtaaaaggc
      361 gattaaaaaa tatatatgaa gatatagaaa aagagaataa tttagtaaag aaattttata
      421 atataagagt tgaatcagaa aatatgattt taaagaaaaa tgcataaaaa atacaactaa
      481 tacagaaata aataaagtaa agttccatgg aaaactgaga aatttaaaaa gagtgtatat
      541 at
//
LOCUS       DAEPIN010000209          537 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.221, whole genome shotgun sequence.
ACCESSION   DAEPIN010000209 DAEPIN010000000
VERSION     DAEPIN010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..537
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.221"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            191..537
                     /locus_tag="KR250_003863"
                     /pseudo
     CDS             191..537
                     /locus_tag="KR250_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430377.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
BASE COUNT          241 a           46 c           85 g          165 t
ORIGIN      
        1 tgcattcact taccactata cataaaaatg taagtaaaga gaagttagac ctattagatg
       61 agtattatag aaacttaaaa gaatgatata gtaaaaatcg aatgtaatta tttaccttaa
      121 ttcctttgtt tgtaaaactt tattgatata gaatttgaca tatacataaa ttggtaaagg
      181 gatgtatact atgaaaatta gtaaattacc agaagcggaa ttaaaagtta tgagatatat
      241 ttgggaatca gaaaaggtat taataccaag agaaatcacc aagtctatgg aacaaaaata
      301 tgaatggaaa gatacaacta tatttacagt attaaaaaga ttactaaaga agagagtttt
      361 tagaagcgga gaaaatagat aagtgtacac actataatgt attagtaaaa aaaaataata
      421 tcagagattt taaacaaaag aatttttaaa gaatacacat aaaaattcaa tattaagttt
      481 aatgtcagca ctatgtagta aggatgatga agtagatggt gatattttaa atttatt
//
LOCUS       DAEPIN010000210          533 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.222, whole genome shotgun sequence.
ACCESSION   DAEPIN010000210 DAEPIN010000000
VERSION     DAEPIN010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..533
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.222"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            <1..>533
                     /locus_tag="KR250_003864"
     CDS             <1..>533
                     /locus_tag="KR250_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HBH0069716.1"
                     /translation="RAMKGSAGSGKSVNVAQDYILKLGDKKYQGANLLVVRKSEATHK
                     YSTYAELTGAINRIYGKQADKYWKTTLNPLEIKSKVTGNSIIFRGVNDAKQREKLKSI
                     NFSKGKLTWVWCEEATELMESDIDILDDRLRGILTNPNLYYQMTFTFNPVSATHWIKR
                     KYFDYKNDDIFTHHSTYL"
BASE COUNT          214 a           69 c           96 g          154 t
ORIGIN      
        1 agagcaatga aaggttcagc agggagtgga aaatctgtta atgtagcaca agactatata
       61 ctaaagttag gagataagaa gtatcaagga gctaatctat tagtagttag aaagtcagaa
      121 gctacacata agtattcaac gtatgcagag cttacaggag ctataaatcg tatttatggt
      181 aaacaagctg ataagtattg gaaaactact ttaaatcctt tagaaattaa gagtaaagtt
      241 actggtaact ctataatttt cagaggagtt aatgatgcaa aacaaagaga aaaattaaaa
      301 tcaattaact tctcgaaagg aaaattaaca tgggtttggt gtgaagaagc tacagaactt
      361 atggaaagtg acatagacat actagatgac cgtttaagag gtattttaac taatcctaac
      421 ctatactatc aaatgacatt tacatttaat ccagtctcag ctactcattg gataaaaaga
      481 aagtattttg actataaaaa tgatgatata tttactcatc atagtactta tct
//
LOCUS       DAEPIN010000211          520 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.denovo.224, whole genome shotgun sequence.
ACCESSION   DAEPIN010000211 DAEPIN010000000
VERSION     DAEPIN010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..520
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.denovo.224"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            <1..>520
                     /gene="cdtR"
                     /locus_tag="KR250_003865"
     CDS             <1..>520
                     /gene="cdtR"
                     /locus_tag="KR250_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family response regulator CdtR"
                     /protein_id="HBH0069717.1"
                     /translation="RIYFIVVDAKYKISNELCDGLWIAQKIRESDYISPIIFLTNHIE
                     MILGIFDYRLEVMDFILKHDMEIAESKIKACIKIAHKRYIKEKNYRSNFFTIYSDFSL
                     WKISFDEVIYFETSTIPHKIKLVTTSRIFEFYKSLKSLPDLDACFIRVHKSFVVNKYH
                     IVSLDLKKNNIKM"
BASE COUNT          209 a           57 c           69 g          185 t
ORIGIN      
        1 agaatatatt ttatagttgt agacgcaaaa tataagataa gtaatgaatt atgtgatgga
       61 ttatggatag cacaaaaaat tagagaatca gattatatta gtccaatcat atttcttaca
      121 aatcatatag aaatgatttt agggatcttc gattataggt tagaagttat ggactttata
      181 ctaaaacatg atatggaaat tgctgaaagt aaaataaaag cttgtattaa aattgctcac
      241 aaaagataca ttaaagaaaa aaattatcgc tctaattttt ttactatcta ttcagatttc
      301 tcactatgga aaatttcatt tgatgaagtt atatattttg aaacaagcac tattccacat
      361 aaaataaaat tagtgactac ttctagaata tttgaattct ataaaagttt aaagtcttta
      421 ccagatttag atgcatgctt tatccgtgta cataaatctt ttgtagttaa taaatatcat
      481 attgtttctc tcgacctaaa gaaaaataat attaaaatga
//
LOCUS       DAEPIN010000212        10877 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.guided.099, whole genome shotgun sequence.
ACCESSION   DAEPIN010000212 DAEPIN010000000
VERSION     DAEPIN010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10877
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.guided.099"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            288..989
                     /locus_tag="KR250_003866"
     CDS             288..989
                     /locus_tag="KR250_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanG-Cd-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HBH0069718.1"
                     /translation="MNENILIVDDEKEIADLIEVYLKNEGFTVYKFYTGKEALECIES
                     KNLDMAILDIMLPDIDGFHICQKIREKYYYPIIMLTAKIEDIDKIMGLTIGADDYITK
                     PFNPLEVVARVKTQLRRYMRYNNSYEQQSIIVNEYDIKGLIINKETHKCSLFGKEVAL
                     TPIEFSILWYLCEHQGKVVPSEELFEAVWGEKYLDNNNTVMAHIGRLREKIHEPSKNP
                     KFIKTVWGVGYTIEK"
     gene            1039..2121
                     /locus_tag="KR250_003867"
     CDS             1039..2121
                     /locus_tag="KR250_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBH0069719.1"
                     /translation="MRTALMAFIAIIFIWILYSFILYGKFSIWIVSFFENAFRMEYKA
                     ALNLYQQIFRNSMELFMILAMVIVFFIIFRVYLNWFTKYFSEINQGIDSLIKEDVGEV
                     ALSPELLAIEKKINSIKHILEQRKFETQMAEQRKNELIVYLAHDLKTPLTSVIGYLTL
                     LRDESQISEELRKKYLSISLDKAERLEDLINEFFEITRFNLSNISLEYSTVSLTRMLE
                     QLTYEFKPTLMSKNLKYTLNVTPNMMIRCDVNKMQRVFDNLLRNAVYYSFEDTTIEIT
                     AVQEDDYIKLKFINHSNTIPEEKLERIFEQFYRLDTSRNSNSGRAGLGLAIAKEIVEL
                     HNGTITAHSENNIITFKVIIPIVGNL"
     gene            2382..3482
                     /gene="vanG"
                     /locus_tag="KR250_003868"
     CDS             2382..3482
                     /gene="vanG"
                     /locus_tag="KR250_003868"
                     /EC_number="6.3.2.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-serine ligase VanG"
                     /protein_id="HBH0069720.1"
                     /translation="MNNKKIAVIFGGNSTEYDVSLQSAFAVLENLNTEKYDIIPIGIT
                     REGNWYHYIGDYNNIQNNTWFERDTDIIPVIVSQNRVKNGIIEFLIPDAVSQNHIPNS
                     AIESCENDIKYTKLDFVFPVLHGKNGEDGTVQGLFELAGIQVIGCGTLSSALCMDKDK
                     SHKLVHSEGIKVPKSILLKYSDKFNVAELSKDLTYPLFIKPVRAGSSFGISKIYKKEE
                     LQVAVDLAFEHDNEVIIEENIDGFEVGCAVLGNNDLLVGRVDEIELSKGFFDYTEKYT
                     LKSSKIHMPARIDLDCEKRIQETAKIIYRTLGCSGFARVDMFLTPSGEIIFNEVNTIP
                     GFTSHSRYPNMMKGIGLSFEEILDKIIEVYIK"
     gene            3479..4285
                     /locus_tag="KR250_003869"
     CDS             3479..4285
                     /locus_tag="KR250_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBH0069721.1"
                     /translation="MMNNILLDKNKEDKMTNTILLTKDKVYDGNLILVNAFLPVKTSE
                     DIDLIPVDTRFPSILMKREATNILQNILKSICGINEIVPVSGYRTAEEQQDIYSSSLR
                     DNGKDFTKKFVALPNHSEHQTGLAIDLGLKKEVIDFICPEFPYNGICNEFRKAALHYG
                     FIERYQCKKEEITGISQEPWHFRYVGYPHSEIINKMNLALEEYIEYIKRFSYTNCLKI
                     EDKEKIIEIFYVPIHTKEEVRISIPEQCVYQISGNNKDGFIVTFWRNRKD"
     gene            4303..6441
                     /gene="vanT"
                     /locus_tag="KR250_003870"
     CDS             4303..6441
                     /gene="vanT"
                     /locus_tag="KR250_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine racemase VanT catalytic subunit"
                     /protein_id="HBH0069722.1"
                     /translation="MIRNKSYAGLDYFRIIAALLIIAIHTSPLLSYSKMADFVLTSII
                     ARIAVPFFFMLSGFFLISTYNYNCNKLKSFLKNVLFIYGIAIVIYIPLNIYTGYFSMN
                     NLVPNIIKDIVFDGTLYHLWYLPASIIGASIAYFLVKKLGLKYSLVITIVIYVIGMFG
                     DSYYGFLEKIPLLKNLYGYIFEVSDYTRNGIFFAPVFFILGGIISNKSNYMSTKNSLI
                     GFLISFLLMLCEGMFLHKLGVQRHDSMYIMLLPCMYFLFTALTFLKGSRARLLRTSSL
                     IIYIIHPMMIVVIRMFSKIFGIQTVLVDNSLIHYIVVSIASFIASIIIILILKPLKKD
                     LKGEHKVNTNRAWIEIDLNNLEHNVKMIQKAMPEDCELMAVVKANAYGHSAFEVATCA
                     NQIGVKAFAVATIDEGIDLRRYGILGEILILGYTNPIRAKELHKYNLTQSVIDLNHAI
                     SLNKQKYNIKSHIKIDTGMHRLGFDVNDVRSILKAFKLKYLDICGIFTHLCVSDSLKD
                     EDVDFTNKQIECFYKLIDFLLKKGIKIPKVHIQSSYGFFNYPNLKCNYVRIGIALYGI
                     LSFPNDTTKLQLDLRPVLSLKSQVILIRKIQKDDSFGYGRVFIASRDSVIAILSIGYA
                     DGIPRNLSCGKSYVIINGCRAAIVGRICMDQLAVDITDIPNVEVGNTAIIIGRDNLSE
                     LSVSEVANNSCSISNELLSRVGRRLNVIKK"
     gene            6755..7375
                     /locus_tag="KR250_003871"
     CDS             6755..7375
                     /locus_tag="KR250_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBH0069723.1"
                     /translation="MQYIQELLDLTQNYWVLATIIGLLSAFIESFIPALPLVAIVTAN
                     AAIQGLFIGCLLSWIGSGLGTTSLFLLISRFNDSKLFNKLRNSKTEKAISWMDKQGFK
                     LLFIAYACPFMPGCLVTIASAFGKKDLKDFVPAMLAGKFVMFIVISYVASDIEGFITS
                     PLKIASFILLVFLSWKIGNRVNKNLENHNYDFHHKKHHNDKDDKMI"
     gene            complement(7541..8077)
                     /locus_tag="KR250_003872"
     CDS             complement(7541..8077)
                     /locus_tag="KR250_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBH0069724.1"
                     /translation="MIKETHERGGYIFALLILPFINNLYLVKYDIAYKIVLIIIYIYF
                     AYLGSLFPDIDMRGSYISKKFMLIYKLFGSRFRHRGFTHSLIALLLIYSFFKSLTIFT
                     NNNIVFSCLSSGFIIGYLSHLCLDLITKEGIELFYPITINISLLPIKTNSKTEKFISK
                     LLNFIVIFLIGYQFYILL"
     gene            complement(8190..8864)
                     /locus_tag="KR250_003873"
     CDS             complement(8190..8864)
                     /locus_tag="KR250_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HBH0069725.1"
                     /translation="MSLFIISQCITSFAFTPENNKFKVKPLPYAYDALEPYIDKETMK
                     LHHDKHYQAYVDKLNAALEKYPELYNYSLCELLQNLDSLPKDIATTVRNNAGGAYNHK
                     FFFDIMTPEKTIPSESLKEAIDRDFGSFEKFKQEFQKSALDVFGSGWAWLVATKDGKL
                     SIMTTPNQDSPVSKNLTPIIGLDVWEHAYYLKYQNRRNEYIDNWFNVVNWNGALENYK
                     NLKSQD"
     gene            9067..>10877
                     /gene="asnB"
                     /locus_tag="KR250_003874"
     CDS             9067..>10877
                     /gene="asnB"
                     /locus_tag="KR250_003874"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HBH0069726.1"
                     /translation="MDGNISIYYKGDISKKEDIKFKIKESVNNTRLSSRIEYKDEEKT
                     FDFERFNVIDAIYGNKPFEKDGKVIVFNGNIYNYHEIKEELIKKGHNFTTNDDIEVLL
                     ASYIEFGKNCVHKIKGMFNFIIYDRENESIFGARDLFGIKPLYYINKENAIIFSSEYK
                     FLLEYMKNLNINERSLQSYFSFQYVLPEDTMIQGIRLIPAGHYFRVENGILSLKRYNK
                     LEFRSSTKFFYTKNHLGNRDVNEDDIRNIVVDSIVTHMEEEKEIGTFLSGGIDSSIIT
                     TVASQINPNIKSFSAGFSVKGYSELEVAKKTADKLGIENIQINITQDEYIKSLPNVIY
                     SLDDPIADPSEVGLYFLIKEAGKHVKVALSGEGADELFGGYNIYKEYSTMKSVVNSPT
                     YIKSILGRVSELMPNIKGRNYLYRATTPLEKRYIGNAKVFENSEVKRFFFKYKEKNIY
                     EYLLSNLYRDAQKNNYDYISKMQHIDVNTWLQGDILQKISKLSTAEQVELRVPFLYKD
                     VFDVAKNLRMEQKINKNNTKVLLREAFREIVPEHVVQRKKLGFPTPIRVWLKDSLGDI
                     VKETIYNSNVDEFIDKKYAIKLLDTHLKGHRDNSRKI"
BASE COUNT         4114 a         1210 c         1794 g         3759 t
ORIGIN      
        1 tttttatatg gattgtctct ggtataataa attgaattgt gtttgactga tagatatttt
       61 tattgaaagt taatacctgt tatgcgataa taaggacgtg ttaggggagt ttttataata
      121 acaattaatc tcagtttttg tgttccaata gtagcatcaa cacattctga tgtagtagtt
      181 cttttcttat aaaaattagt tacaaatatg cttgtaatta tttgatatgt aaatttacca
      241 atattataaa tgatgacctc gataataagg agagtgaaaa ataatctatg aacgaaaata
      301 ttttgattgt ggatgatgaa aaagaaatag cagatttaat tgaagtatat ttaaaaaatg
      361 aaggttttac agtatacaag ttttatacag gtaaggaagc tctagaatgt attgaatcta
      421 aaaatttgga tatggctatt ttagatatta tgcttcctga tattgatgga tttcatattt
      481 gccagaaaat tcgagaaaaa tactattatc ctatcattat gttaacagct aaaattgaag
      541 atatagacaa gataatgggt ttaaccatag gtgctgatga ttatataaca aagccattta
      601 accctttgga agttgttgca agagtaaaga cacaattaag aagatatatg cgatataata
      661 attcttatga acaacagtcg ataattgtta atgagtatga tattaaagga ttgattatca
      721 ataaagaaac acataaatgt agtttatttg ggaaagaagt agcattaaca ccgattgaat
      781 tttctattct ttggtattta tgtgaacatc aaggaaaagt ggttccttct gaagaattgt
      841 ttgaagctgt atggggagaa aaatatcttg ataataataa cacagttatg gctcatatag
      901 ggcgattacg tgaaaaaatt catgaacctt ctaaaaaccc gaaatttata aaaactgtat
      961 ggggagtagg ttatacaatt gaaaagtaaa aatattaaag tcaatcctga ctttactcaa
     1021 gtgagaaata aaatttttat gagaacagct ttaatggcat ttatagccat tattttcata
     1081 tggattttat attcttttat attgtatggt aagttttcta tctggatagt atcattcttt
     1141 gaaaatgctt ttcgtatgga atataaagct gcattgaatt tatatcagca gattttccgt
     1201 aatagtatgg agctcttcat gatactagct atggtgattg tgttttttat aatattccgt
     1261 gtttatttaa attggtttac aaaatatttt agtgaaataa atcaaggtat tgactctctt
     1321 atcaaagaag atgttggaga agtggcttta tctccagagt tattggcaat agaaaagaaa
     1381 ataaactcaa ttaaacatat acttgaacaa cgtaaatttg agacacaaat ggcagaacaa
     1441 cgtaagaatg aacttattgt ttatcttgca catgatttaa aaacaccact tacatcagtt
     1501 atagggtatc tgacactact tcgagatgaa agtcagattt ctgaagaact gcgaaaaaaa
     1561 tatttatcta tttcgttgga taaggcagag cgtcttgaag atttaattaa tgaattcttt
     1621 gaaattacaa ggtttaattt atcaaatata tcacttgaat acagtacagt aagcttaact
     1681 cgtatgcttg aacaactaac atatgagttt aaacctactt tgatgagtaa gaatttaaaa
     1741 tatacactga atgttacacc aaatatgatg ataaggtgtg atgttaataa aatgcaacgt
     1801 gtatttgata acttattacg aaatgctgtt tattatagtt ttgaagatac tactattgaa
     1861 ataacagctg tacaggagga tgattatata aagcttaaat ttataaatca tagcaatacg
     1921 attccagagg aaaaacttga gcgtattttt gaacaatttt acaggttgga tacatctcgt
     1981 aattcaaaca gtggtagagc tgggttggga cttgctatag ctaaagaaat agtggagctt
     2041 cataacggga caataacagc tcatagcgag aacaatataa ttacctttaa ggttattatt
     2101 ccgattgtag gaaatttgta agaatttaag tataaaaaaa taggaaattc tttaggataa
     2161 ataaataaag agcactcttg ttattgatat aattactgta tcaaaacaag agtgctttta
     2221 ttttgataca gtaattatat caatagaaaa atgtgaaaat atattatgtg gaacagatgc
     2281 aattgtaagt gaaggttgat tggatttgaa atagaaaaag tattttaaca tgtttctgta
     2341 gattgtataa aaataaataa atcaacaaag gagaatcaaa gatgaataat aaaaaaattg
     2401 cagttatatt tggtggaaac tcaacagaat atgatgtatc attacaatca gcttttgcag
     2461 tacttgagaa tttgaatacg gaaaaatatg atattatacc aattggaatt acaagggagg
     2521 gtaattggta tcattatatt ggagattata ataatatcca gaataacact tggtttgaaa
     2581 gggatactga tataattcca gttattgttt cgcagaaccg tgtcaaaaat ggtattattg
     2641 aatttttaat tcctgatgct gtttctcaga atcatattcc aaatagtgct attgaatcat
     2701 gtgaaaatga tattaaatac actaaacttg attttgtgtt tcctgtactg catggaaaaa
     2761 atggagaaga tggaacagtg caggggttat ttgaattggc aggtatacag gttattggat
     2821 gtggtactct ttcttcagca ttatgtatgg acaaagacaa atcacataaa cttgttcatt
     2881 cagaaggtat taaagtacca aaatcaatat tactaaaata ctcagataaa tttaatgtgg
     2941 ctgaactgtc aaaagactta acttatccat tatttataaa acctgtgcgt gcaggttcat
     3001 catttggtat tagtaaaatt tataaaaaag aagagttaca agtagcagta gacttggctt
     3061 ttgaacatga taatgaagta ataattgagg aaaatattga tggttttgag gtagggtgtg
     3121 cagttttagg aaacaatgac ttacttgttg gaagagtaga tgaaatagaa ttgtcaaaag
     3181 gattttttga ttatacagaa aaatatacat tgaaaagttc aaaaattcat atgccagcta
     3241 gaattgattt ggattgtgag aaaagaatac aagagacagc aaaaattatt tatagaactc
     3301 ttggatgttc tggatttgct agagtagata tgtttttaac tccatctggt gaaattattt
     3361 ttaatgaagt caatacaatt cctggcttta cttctcatag tcgttatcca aatatgatga
     3421 aaggtattgg actgtctttt gaagaaatat tggacaagat aattgaggtg tacataaaat
     3481 gatgaataat attttattag ataaaaataa agaagataaa atgacaaata ctatcttatt
     3541 gactaaagat aaagtttatg atgggaatct aattctagta aatgcttttt taccagttaa
     3601 aacatctgaa gatatagatt taattcctgt agatacaagg tttccaagta ttcttatgaa
     3661 acgtgaagct acaaacattt tacaaaatat tttaaaaagt atatgtggaa taaatgaaat
     3721 agtacctgta agtggatatc gtactgcaga ggaacaacaa gatatatatt caagttctct
     3781 gcgtgacaat ggaaaagact ttactaaaaa gtttgtagca cttccaaatc acagtgaaca
     3841 tcagacaggg cttgctattg atttagggtt aaagaaagaa gttatagatt ttatctgccc
     3901 tgaatttcct tataatggta tttgtaatga gtttcgtaag gcagcactac attatggttt
     3961 tatagagcgt tatcagtgta aaaaagaaga aataacagga atatctcaag aaccatggca
     4021 ttttcgttat gtgggatatc cgcattctga gattatcaat aaaatgaatc tcgctcttga
     4081 agaatatata gaatatatta agagattttc gtatacaaat tgtttaaaaa tagaagataa
     4141 agaaaaaatt atagaaatct tttatgtgcc tattcatact aaagaagaag tgagaatctc
     4201 tatacctgaa caatgtgtat atcaaatatc gggtaacaat aaggatggtt ttattgtgac
     4261 attttggaga aatagaaagg actaagaaat ggaggaacaa gaatgattag gaataaatct
     4321 tatgcagggc ttgattattt taggattata gctgcattac ttataattgc aattcataca
     4381 tctccattat tatcatatag taaaatggct gactttgtgt taactagtat aattgctcgt
     4441 attgctgtgc cattcttttt tatgctatca ggcttttttc taatttcaac atataattat
     4501 aattgtaata aactgaaatc ttttctaaaa aatgtattat ttatttacgg gatagctatt
     4561 gtaatttata ttcccttaaa tatttacact ggatattttt ccatgaataa tttagtgccc
     4621 aatattatta aggacattgt atttgatggg actttatatc atttatggta tcttcctgca
     4681 tcaattattg gtgcaagtat tgcatacttt cttgtaaaaa aactaggttt aaagtactca
     4741 ctagtaatca ctattgttat ttatgtaatt ggtatgtttg gtgatagtta ttatggtttt
     4801 ttagaaaaaa taccactatt aaagaattta tatgggtata tttttgaagt atcagattat
     4861 acacgaaatg gtatattttt tgcacctgta tttttcatac ttggtggaat aatatctaat
     4921 aaatctaatt atatgtcaac taaaaatagc ttgattggat ttcttatatc attcttactt
     4981 atgttatgtg agggaatgtt tttacataaa ctaggggtac agcgtcatga tagcatgtat
     5041 attatgttat tgccatgtat gtatttctta tttacagcct taacattttt gaaaggttct
     5101 cgagctaggt tattgcgaac atcatcatta attatttata ttatacatcc tatgatgatt
     5161 gttgttattc gtatgttttc taaaatattc ggaatacaaa ctgtcttggt tgacaatagc
     5221 cttattcatt atatagtggt aagtatagca tcttttatag cttctataat tattatattg
     5281 attcttaaac ctctaaaaaa agacttgaaa ggagaacata aggtaaatac caatagagct
     5341 tggattgaaa ttgatttaaa taatttggaa cataatgtaa agatgataca aaaagctatg
     5401 ccagaagatt gtgaattgat ggcagttgta aaagctaatg cctatggtca tagtgctttt
     5461 gaagtagcga cttgtgcaaa ccaaataggt gtaaaagcat ttgcagtagc aacaatagat
     5521 gaggggattg atttacgtcg atatggaata ctaggagaaa tattgattct tggatatact
     5581 aacccaataa gggcaaagga acttcataaa tacaatctta cacagagtgt tattgacctt
     5641 aatcatgcta tctctttaaa caaacagaaa tacaacataa aatcacacat taaaattgat
     5701 acaggtatgc acagactggg ttttgatgtt aatgatgtga ggtcaatttt aaaggcattt
     5761 aaattaaaat acttagatat atgtggcata tttacacact tgtgtgtttc agatagtctg
     5821 aaagatgaag atgtagactt tacaaataaa caaattgaat gtttttataa gttaattgat
     5881 tttctgttaa aaaaaggtat aaaaattcct aaagtacata ttcaaagtag ttatggtttt
     5941 tttaattatc ccaatcttaa gtgtaattat gtaagaattg gaattgcatt atacgggata
     6001 ttaagttttc caaatgatac gactaaattg caacttgatt tacgacctgt tttatcctta
     6061 aaatcacaag taattcttat tagaaaaata caaaaagatg atagttttgg atatggaaga
     6121 gtctttattg caagcagaga tagcgtaatt gctatattat ctattggcta tgccgatggt
     6181 atacctagaa atttatcctg tggaaaaagt tacgtaataa taaatggttg tagagcagca
     6241 atagtgggtc gaatttgcat ggaccaactt gctgttgata ttacagatat acctaatgta
     6301 gaagttggta atacggcaat aataatagga agagataatt tatctgaatt atctgtttct
     6361 gaagttgcaa ataactcatg tagtatatcg aatgaattat tgagcagagt aggaagaagg
     6421 ttaaatgtta taaagaagta actcaattaa atagtaatat ttgttgtact gtgtaattta
     6481 ttttactctg actagacatt tttagtaata ttatgtctaa tatatatttt ttataactta
     6541 catgacaatt taagttataa aaaggtgatg tttcagattt ttattttgaa acatcacctt
     6601 ttttgttata atatataata aaataattat accttaactg taaacttata tagattattt
     6661 actttttaat attagattca ttttttgaga ttggtatata ataaacatat agagtatata
     6721 ataatagtta aagttaaaaa aggggaaaag caatatgcag tatatacaag aactacttga
     6781 tttgactcaa aattattggg tgttagccac aataatcggc ttattaagtg catttataga
     6841 aagctttata ccagccttac cacttgttgc tatagttaca gcaaatgctg caatacaagg
     6901 attatttatt ggatgtttgt tatcatggat tggctcagga cttgggacaa cttcattatt
     6961 cttattaata agcagattta atgatagtaa gctttttaat aagttgagaa atagtaaaac
     7021 tgaaaaagca ataagttgga tggataaaca gggatttaag ttattattta tagcgtatgc
     7081 ctgtccattt atgcccggtt gtttagttac tatcgcatca gcttttggta aaaaagattt
     7141 aaaagatttt gtgccagcaa tgttggcagg aaaatttgta atgtttatag ttataagtta
     7201 cgtggcaagt gatatagaag gttttataac aagtccttta aagatagcat catttatatt
     7261 attggtgttt ttgtcatgga agattggaaa tagagtaaat aaaaatcttg aaaatcataa
     7321 ttatgatttc catcataaaa aacatcacaa tgataaagat gataaaatga tttagcttgt
     7381 acgaaataaa acgtatctta aaatgattta gtactaaatt attttaagat acgttttatt
     7441 ttttgtatta aggtacaaaa tttttagttc ttttgtaaac taaaatatat aaaacttatt
     7501 tatacttagt tcaaaatatt gttatttatt ttttctacaa ctataataaa atataaaatt
     7561 gatatccaat caaaaaaata actatgaaat ttaatagctt agatatgaat ttttctgttt
     7621 ttgagttagt ttttataggt agtaaagaaa tgtttatagt aatgggataa aacaattcaa
     7681 taccttcttt agtaataagg tcaagacaca agtgagatag atatcctatt ataaacccac
     7741 tggacaaaca tgagaaaact atattattgt tagtgaagat tgtcagagat ttaaaaaaac
     7801 tataaataag taataaggca attagactgt gagtaaaccc tctatgtcta aacctacttc
     7861 caaataattt gtatattaac ataaattttt tacttatgta agaacctctc atatctatat
     7921 ctggaaataa agaacctaaa tatgcaaaat aaatataaat tataattaat actattttat
     7981 atgcaatatc gtatttaact aggtacagat tgtttataaa ggggagtatc aaaagtgcga
     8041 atatataacc tcccctttca tgtgtttctt ttatcatata aaaatcctcc tgaatcgtca
     8101 ctataaacat tatattggta aatttagact atcttttatt atattatata gcaaacataa
     8161 gtttttttca aaaggatttt aatatttaat taatcttgag attttaaatt tttgtaattt
     8221 tctaaagcac cattccaatt tactacatta aaccagttgt caatgtattc atttcttcta
     8281 ttttgatatt ttaaatagta agcatgctcc caaacatcaa gtcctattat aggagttagg
     8341 tttttactta cagggctatc ctgatttgga gtagtcataa tagataattt cccatcttta
     8401 gtagctacaa gccaagccca accagaacca aagacatcta aagcagattt ttggaactct
     8461 tgcttaaatt tttcaaaaga accaaagtct ctatcaatag cttcttttaa agattcagaa
     8521 ggtatggttt tttctggcgt cattatatca aaaaagaatt tatgattata agctccacct
     8581 gcattatttc ttacagttgt agcaatatct ttaggtaaag aatctaaatt ttgcaataat
     8641 tcacataaag aataattata aagctcagga tatttttcaa gagcagcatt taatttatca
     8701 acataagctt gataatgctt atcatgatgc agtttcattg tttctttatc tatataaggt
     8761 tcaagtgcat catatgcata aggtaatggt ttaaccttaa atttgttatt ttcaggtgta
     8821 aaagcaaatg aagttatgca ctgtgagatt ataaataaag acataataac tggtattaat
     8881 atttttttct tcataaaaat cacctcctaa tatagtattt ctagaaataa aaaaaatatg
     8941 aaatattaac agataattta taagaattta atagcaattt cattttaaat tgaataagtt
     9001 ttaaagccaa agacaaaact atgaaatata gaataagact aaaggacaga aaggaatttt
     9061 gattatatgg acggcaatat ttcaatttat tataaaggtg atatatctaa aaaagaagat
     9121 attaaattta aaataaaaga atctgtaaat aatacaagac tttctagtag aatagagtat
     9181 aaggatgagg aaaaaacttt tgattttgaa agatttaatg taattgatgc tatttatgga
     9241 aataaaccat ttgaaaaaga tggaaaagtt attgttttta atggaaatat atataactat
     9301 catgaaataa aagaagaatt aattaaaaaa ggacacaatt ttactacaaa tgatgatatt
     9361 gaagtcttat tggcatctta tatagaattt ggtaagaatt gtgtacataa gattaaagga
     9421 atgttcaact ttataatcta tgatagagaa aatgaaagta tatttggagc aagagattta
     9481 tttgggataa aaccactcta ttatataaat aaggaaaatg caataatttt ttcctcagag
     9541 tataagtttt tacttgaata tatgaaaaat ttaaatataa atgaaagaag cttacaaagt
     9601 tatttttcat ttcaatatgt acttcctgaa gatactatga tacaaggaat aaggctgata
     9661 ccagcaggtc actattttag ggtagaaaat ggtatactat ctttaaagag atataataaa
     9721 cttgagttta gaagtagtac aaaatttttt tatactaaaa atcacttagg caatagagat
     9781 gtgaacgagg atgatataag aaatatagta gtagattcaa tagtaactca tatggaagag
     9841 gagaaagaaa ttggaacatt tctgtctggt ggaatagatt catctattat aactactgtg
     9901 gcttcacaaa ttaatccaaa tataaaatct ttttctgcag gctttagtgt caaaggatat
     9961 agtgaattag aagtagcaaa gaaaacagct gataagttag gtatagaaaa tatccagatt
    10021 aatataacac aagatgaata tataaaatca ttaccgaatg taatttattc tttggatgac
    10081 cctatagcag acccttcaga agtaggtctt tatttcttaa ttaaagaggc aggtaaacat
    10141 gttaaagtag cattgtctgg agaaggagca gatgaattat ttggaggata taatatatat
    10201 aaagagtata gtactatgaa atcagttgtc aattcaccta cttatattaa aagtatatta
    10261 ggaagagttt cagaacttat gccaaacata aaaggaagaa attatcttta tagagctaca
    10321 acaccattag aaaaaagata tataggaaat gcaaaagttt ttgaaaacag cgaagtaaaa
    10381 agattctttt ttaagtataa ggaaaagaat atatatgaat acctgttgtc taatttgtat
    10441 agagatgcac aaaaaaataa ttacgattat ataagtaaaa tgcaacatat agatgtgaat
    10501 acatggcttc aaggcgatat acttcaaaaa attagcaaac tttctacagc tgagcaggtt
    10561 gaacttagag taccattttt atataaagat gtatttgatg tagcaaaaaa tttaagaatg
    10621 gagcaaaaaa taaataagaa taatacaaaa gttttactta gagaggcgtt tagagaaata
    10681 gtcccagaac atgttgttca aaggaaaaaa ttaggatttc caactccaat aagagtttgg
    10741 ttaaaagatt ctctagggga tattgtaaag gaaactattt ataattcaaa tgtagatgaa
    10801 tttatagata aaaaatatgc aataaaactt ttagatacgc atcttaaagg tcatagagat
    10861 aattcgagaa aaatatg
//
LOCUS       DAEPIN010000213        10790 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.guided.100, whole genome shotgun sequence.
ACCESSION   DAEPIN010000213 DAEPIN010000000
VERSION     DAEPIN010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10790
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.guided.100"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            complement(484..1299)
                     /locus_tag="KR250_003875"
     CDS             complement(484..1299)
                     /locus_tag="KR250_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase family protein"
                     /protein_id="HBH0069727.1"
                     /translation="MVKLSDAKSWAIRMSEDGCAYKSIDNYKRSLKASFFMAIQDDCI
                     RKNPFNFQLSDVLEDTTEPKIALTPEQEMGLLSFMESDKTYCKYYDDVVILLETGLRI
                     SELCGLTLELDMKNKAIMVDHQLLSDTKIGYYVEVPKTTQGKREIPMTERAYQSFERI
                     VERRKNAKAEPIIVDGYQNFLFLKQDGLPKQATHYQSMLRALVKKYNKTHEDKLPKIT
                     PHTFRHTLCTNMANKGMSPNILQYIMGHKNITMTLGYYAHGSFSSSRAEMERL"
     gene            1391..2659
                     /locus_tag="KR250_003876"
                     /pseudo
     CDS             1391..2659
                     /locus_tag="KR250_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            2758..3378
                     /locus_tag="KR250_003877"
     CDS             2758..3378
                     /locus_tag="KR250_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBH0069728.1"
                     /translation="MHLIDFMNEDIKKKLVKKTYKKKETILFAEQENEYVILMIDGIA
                     EAFILNVKGNISTIHLYRSGSFFGEMEQFNDGKKPVEIVAFTDCNVYKLHRDDFLNWL
                     RGDFEATKFLIREISSKLVVNAELIEELSSLSVKERLLRCISLHYYRSELNLLTKKQL
                     ASEVNAPIRSINRAINECVQEHLIAYENKRFSVINQREVLKNLPTT"
     gene            3528..3932
                     /locus_tag="KR250_003878"
                     /pseudo
     CDS             3528..3932
                     /locus_tag="KR250_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433844.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oxalate:formate antiporter"
     gene            4057..4983
                     /locus_tag="KR250_003879"
     CDS             4057..4983
                     /locus_tag="KR250_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0069729.1"
                     /translation="MSDILSRITNKIGDKNIVDKLSALSKSDLNSLLLEVFDRQANTL
                     TATDILKSYQLNRFTIPSGIDPEEIHALESKLLRKAFNMDIKTIMLSPSAPLGSCSVF
                     GSVDQYNVVSALRGTETLADPSNMLAIIIADKLKRKEENNTIPIHMATTARVIRAQNF
                     VGKGLYSHFGLYCMVSSGIDTGSYTCEKMLLEKHLNYYKDILSEIENVRFSIILRKRN
                     GYKDNDGFFDRMVETIKLIFPNIELSIHNDDVDNNYYKGINFKLSVKQGNETVEIVDG
                     GFVDWTYQMLGNKKERCLISGIGLERFLVAVS"
     gene            4997..5446
                     /locus_tag="KR250_003880"
     CDS             4997..5446
                     /locus_tag="KR250_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBH0069730.1"
                     /translation="MELIEWNYAYITDLVEFANDKEIAKNLRDNFPHPYREQNARDFV
                     SFCLATPDVKQLSYAIFYQERAIGSISLTFEEDTSINKNAELGYWLARNYWGKGIATN
                     AVKEICKIAFEKYNLNKIHANVYSYNRGSSKVLEKCGFKIEEYCHNS"
     gene            5806..6213
                     /locus_tag="KR250_003881"
     CDS             5806..6213
                     /locus_tag="KR250_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBH0069731.1"
                     /translation="MVDEKTIEHQFDSFCKTVLRNYARDIYDENKRRNDYLVSLESLS
                     LAELSKLSILDDYDSNYICMVSYDYNIRIEDVLMAQAIGKLTKRKQDIILLSFFLNMT
                     NADIATLMDLAENTIHYHKTNALKELKELMEEH"
     gene            6215..6445
                     /locus_tag="KR250_003882"
     CDS             6215..6445
                     /locus_tag="KR250_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH0069732.1"
                     /translation="MKPTKEVLPFTIILLAVHGDVNAINGILKHFEHYIIRLSQKTLF
                     DEYGNPYIHVEEETKRMLGTKLITAILRFNLS"
     gene            6835..7038
                     /locus_tag="KR250_003883"
     CDS             6835..7038
                     /locus_tag="KR250_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HBH0069733.1"
                     /translation="MNNTDVPIWEKYTLTIEEASKYFRIGEKKLRKLADENLDSGWVI
                     MNSNRIQIKRKQFEKIVDTLDFI"
     gene            7116..7469
                     /locus_tag="KR250_003884"
     CDS             7116..7469
                     /locus_tag="KR250_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase DNA-binding domain-containing protein"
                     /protein_id="HBH0069734.1"
                     /translation="MSTEKRRDNKDRILRTGESQRKDGRYAYKYVYSFGKPQFVYSWK
                     LVPTDKPPKGKRDDISLRDKEKAIQNDLDDSIDTIGKKMTVCQLYAKKNGLRKNIRLN
                     TKKGRHYLMKILNKE"
     gene            7541..8173
                     /locus_tag="KR250_003885"
     CDS             7541..8173
                     /locus_tag="KR250_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HBH0069735.1"
                     /translation="MTIPSSNKVYPRSNDYQTVYLKNVITRENIEVGDYTIYNDFYND
                     PRDFEKNNVLYHYPINNDKLIIGKFCSIACNAKFLMTSGNHSMKSKSTYTFPIFYEEW
                     KLDINHITDAWDNKGNIIIGNDVWIGYDSIIMSGVNIGDGAIIGTRAVVTNDVPPYSI
                     VGGVPAKIIKKRFNNDTISKLLEIKWWDWPYKKIQSNIKHIQSGNIDKLV"
     gene            complement(8544..9413)
                     /locus_tag="KR250_003886"
     CDS             complement(8544..9413)
                     /locus_tag="KR250_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBH0069736.1"
                     /translation="MEYDMPLYRPPSEAYSLIIQVTLGCSHNKCTFCSMYKSKKFKIK
                     PLEVIKNEIDIFRRYYKHVERIFLADGDALIIPMEKLRDIILYIKEVFPECERITMYG
                     SPKSIEKKSDDELKELRKLGVKMIYLGLESGSNEVLEDIKKGFSSEQLIKVGRKVKKS
                     GIKLSATVIAGLGGTKKTHQHAVDTGKMLGAISPDYVGVLSLMVEPNTELYDLLKAGE
                     FTILEDKAVLQEIKEMIKNIDANEKIVFRSNHASNYANLKGVLPDDKQRLIDEIDYYL
                     SNQKLKREEYRRL"
     gene            complement(9510..10367)
                     /locus_tag="KR250_003887"
     CDS             complement(9510..10367)
                     /locus_tag="KR250_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBH0069737.1"
                     /translation="MSKDVKVKRNKKYDTFSITMELERPLLYDEVENKDDKNNNRKFN
                     IKNKIFKGIGILFVLILLGFFIWINKTYEPQKLAKEALVSNSKVEVTVNENISFTPKG
                     RKVSKGLILYPGAKVEIESYAPLARKIAESGYEVVIVKMPLNLAILGTNKAQKVMDSY
                     NNIEHWVIGGHSLGGVAASNFARDNKLIEGVVFLASYPMGDELKELGKKVISIWGSKD
                     GVVNFKSLVESKQKLPDDTTYVEIEGGNHAQFGDYGKQKGDNDAIISQEKQLNITANS
                     IIKFLKNIS"
     gene            complement(10582..>10790)
                     /locus_tag="KR250_003888"
     CDS             complement(10582..>10790)
                     /locus_tag="KR250_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBH0069738.1"
                     /translation="KILRDTNYISNYNVDKYDEGRSVILESIVNTKSLGDKIICLNLL
                     QTLNGVKEMYRISNITKVSIKEAA"
BASE COUNT         3682 a         1660 c         1685 g         3763 t
ORIGIN      
        1 tctttttcat aattctaaat gctcctttca ttttgggtaa taaaatagcc gtccataatg
       61 aacggctgga aatagaaaag gaacgtcaat ttaggcgttc catagtctat gaaatattca
      121 atttttttct tgcttcaata ctgatgtgcc gtaccatatc tttgcatatc taggaaaact
      181 tgcgtatcaa ggctcattca ttggtagtaa attagtagta aattgatgtg tttgtttcct
      241 tgaaaatgct gatagatact atgttttagc ggataatcaa ctttttagta tgtttttatt
      301 tgaaaaactt atattaaatt tgattatctt ttcataagtt ggtaaatgtc gatatatcct
      361 gtatctacaa ggagtatcgg cattttcttt ttggaagata ctttgcataa gttggcattt
      421 aaccgtataa gaatgccatc aaaagtagta aataagtagt aaactctagt catactacta
      481 ggattataac cgttccattt cagctctcga agaactaaat gaaccatgtg cataatagcc
      541 aagcgtcata gtaatgtttt tatgccccat aatatattgc aatatattag gactcattcc
      601 tttgttagcc atatttgtac agagtgtatg cctaaaggta tgtggcgtaa tctttggtag
      661 cttgtcctca tgggtcttat tatacttctt gactaacgct cttaacatgc tttgataatg
      721 agttgcttgt tttggtaatc catcttgttt aagaaatagg aagttctggt aaccatcaac
      781 aatgattggt tctgctttag cgttcttacg ccgttccacg atacgttcaa atgattgata
      841 agctctttca gtcattggaa tttctcgctt accctgtgtg gtctttggaa cttctacata
      901 atagccaatc ttagtatcac tcaacaattg atggtctacc ataatggctt tatttttcat
      961 atccaattct aaagtcaaac cgcataattc agaaatgcgt agtcctgttt ctaagaggat
     1021 aacaacatca tcataatatt tacaataagt cttatcgctc tccataaagg ataataagcc
     1081 catttcctgt tctggtgtta gagcgatttt tggttctgtt gtatcttcca aaacatcact
     1141 taactgaaaa ttaaatggat tctttctaat acagtcgtct tgaattgcca tgaaaaacga
     1201 cgctttcagt gagcgcttat aattgtcaat ggatttataa gcacacccat cttcactcat
     1261 tcggatagcc cagcttttag cgtctgataa tttaaccata tcaatgcttt ttgcacctag
     1321 tgggtcattg atagtatcta tattttgatt tataatttat acattttttt aaaaacagac
     1381 ttttaactgt aaagaatcta tgtataaact tagctatgtt gtccgtgtat ctacaaagga
     1441 tcttgacgag ctaaaaaggc gttgtgatga agtcaaagac ttttacgacg acttatcact
     1501 caagctcgtg cgtccctttg gggatatgtt gggattgcat ggcgaattta ttcctgtcag
     1561 caaacgttac atcaatgatt atattcaata tgtcacttcg gattttttgg ctggacttgg
     1621 ttttggagcg acacaaaccc ttggcgaaca tgaggatatc tatcttggct acaatctgga
     1681 cactggaaag aatgtctatc taaaacctgc ccttgctagt caatgggtaa agggttctat
     1741 taccaatgtg ttagcgtctg cgtttatcgg ttcattgggt ggtggtaaat tattcagtaa
     1801 caatatgctt gtttactatg cggtactatt cggcggacag gcggtcatag ttgaccctaa
     1861 agcagaacgc ggcaaatgga aagaaacgct accagaaata gcccatgaga taaacattgt
     1921 caatttaacc agtaatgatg aaaacaaagg attgcttgac ccttacgtca ttttgagccg
     1981 taaaaatgat tctgaaagtt tagcgattga tatcctaaca ttccttgctg gtatctccag
     2041 tcgtgacagt gataagttcc cagtgcttag aaaagcaatc cgcaatgtaa cccaaagtga
     2101 acagcgaggg ttattacttg tcattgatga attacgaaaa gaagatactt ctatcagtaa
     2161 cagcatagcc gaccatatcg aaagttttgt agactatgat tttgcacatc tactgtttgg
     2221 tgacggaaat gtgaagcagt ctatcagctt ggagaaacag ctgaatatta ttcaagttgc
     2281 tgacctcgta ttgccagatg ccaacactag ctttgaagaa tatacaacaa tagaacttct
     2341 tagtgttgct atgttgattg ttattagtac ctttgcatta gactttattc atactgaccg
     2401 ctctagcttc aagatagttg accttgacga agcatggtct tttttgcagg tggcacaagg
     2461 taaaacgcta tctatgaaac tggttagagc atcgcaagat tcacaggaca aacgaattga
     2521 acgtttgaag aattatctga ctgaaaacct acaacagttg aatactgaaa tggttcgctc
     2581 atatattcct actagttcta ttgtcaacag ctttctcagt ttgagcttac tttgcagaaa
     2641 aatgacaact ggaaaataat aatggaatag tttctatgta aatatatttt tactatctta
     2701 cctaactaat tgaaagatga tataatttaa tcaaagattt ggtaaggagg gatgaatatg
     2761 catttaattg attttatgaa tgaagatatt aaaaagaagc ttgttaaaaa aacgtataaa
     2821 aagaaagaga ctattctttt tgctgagcaa gaaaatgaat atgtaattct tatgattgac
     2881 ggtattgcag aagcattcat cttaaatgtg aaaggaaaca tctcaactat tcatctttat
     2941 agaagtggta gtttttttgg agaaatggaa caatttaatg atgggaaaaa acccgttgaa
     3001 attgtcgctt tcacagattg taatgtatat aaacttcata gagatgattt tttaaactgg
     3061 ttaaggggag attttgaagc tacaaaattt ctgattcgag aaatatctag taaattagtt
     3121 gtaaatgctg aattaattga agaattatca tctttaagtg ttaaagaacg acttctgagg
     3181 tgtatatcct tacactatta tagaagtgaa ttaaatctgc ttaccaaaaa acagcttgct
     3241 agcgaagtaa acgctccaat tagaagcatt aatcgtgcta taaatgaatg tgtacaagaa
     3301 catctaattg cttatgaaaa caaaagattt agtgtaatta atcagcgaga ggtattaaag
     3361 aatcttccta caacataagt ataatttcaa aaaagtattg aataataatt attcaatgct
     3421 tttttatttc gggtcaagtg acctattttt ttttattttt tcattatata atactcctat
     3481 gcataattat gttggattga tagctaataa aagaaagtga gaggtgttat actaaaaaag
     3541 aagcatactt cccaactcca gacttacagt ggattgaaga tagattttgg atttgggttc
     3601 attacgcttg cactaagatt ggacgtagag aactatttga aacaattgat tttttatctt
     3661 ttttaagaca atcagtactt gccccattaa tacagcttct cttaggtaaa ttacctcgtg
     3721 gagttagaaa aattgaattg gacgcacccc aatatattga agcattagaa aagacggttg
     3781 ctaaccatga atataaaagc tgtgttaatg cattgcaggc tactatcaac ctgtatcttg
     3841 agttaagaga ggaattatca caaagtccta ttgtccgaaa ggttgaagct gaaagggttg
     3901 cattacagta ttttcaagat ttaatcaaat aaacccaaag taaaaaaata ttgaatagta
     3961 aatattcagt gttttttcat ttttggtcaa atgacctaga ttagttgttg taaccattct
     4021 ataattattt tgaaagtaac agtatggagg ataactatga gtgatatttt aagtagaatt
     4081 acaaataaaa ttggggataa gaatatagtt gataagttat cagctctatc aaaatcagat
     4141 ttgaactcac ttctattaga agtgtttgat agacaagcaa atactttaac tgcaactgat
     4201 attctaaagt cataccaact aaatagattt actatcccta gcggtataga tccagaagaa
     4261 attcatgctt tggagtcaaa attattgaga aaagcattca atatggatat caagacgatt
     4321 atgctttctc cctctgctcc attaggtagc tgtagcgttt ttggctccgt agatcaatat
     4381 aatgtagtaa gtgctctacg agggacagaa acacttgcag acccctcaaa tatgttggca
     4441 ataattattg ctgataaatt aaaaagaaaa gaagaaaata ataccatacc tatccacatg
     4501 gctactactg ctagggtaat aagagcacaa aattttgtag gtaaaggttt atattcacat
     4561 tttggcttat attgtatggt ttcatctggt atagatacag ggtcttacac ttgtgaaaaa
     4621 atgttgttag aaaagcactt aaactattat aaagatatat taagtgaaat agaaaatgtt
     4681 cgtttctcta ttattctacg caaaagaaac ggatacaaag ataatgacgg atttttcgac
     4741 agaatggttg aaactatcaa acttattttt cctaatatag agttatcaat tcataatgat
     4801 gatgttgata ataattatta taaaggtatc aatttcaaat tatcggtcaa acaaggaaat
     4861 gaaaccgttg aaatagttga cggtggattt gttgactgga cttatcaaat gcttggaaat
     4921 aaaaaagaac gctgtttgat aagtggtatt ggcttagaaa gatttcttgt agctgtttca
     4981 taaaaggaga agaaaaatgg aacttataga gtggaattat gcttatatta ccgatttagt
     5041 tgagtttgcg aatgataaag aaattgcaaa gaatcttcgt gacaacttcc cccaccctta
     5101 tagggagcaa aacgctagag attttgttag tttttgtttg gcaactccag atgttaaaca
     5161 acttagctat gctatttttt atcaagaaag ggctattgga agtatcagcc taacatttga
     5221 agaagatacc tctattaata aaaatgctga attaggttac tggcttgcta ggaactattg
     5281 gggcaaaggg atagcaacaa atgcagtaaa agaaatatgc aaaattgcct ttgaaaaata
     5341 caaccttaac aaaattcatg ctaatgtata ttcctacaat agagggtcaa gtaaagtatt
     5401 ggaaaaatgt ggttttaaga ttgaagaata ctgtcacaat tcataataaa gaatagaaac
     5461 atgattgatt gccatgtata tagtttaatt cgaccgcaat agcatttatt agaattaacc
     5521 tactttttaa taacagaata agtttttccc ctctattaat ttcttatgca tctttgtatt
     5581 tttatgaaaa gctagccaat gcatatttcc atacatcaat atggtggtgg cttacaacca
     5641 atataaatta aatacgtttt aaagaataca catttttgcc ctataggcag agtgtgtatt
     5701 tttttctctt ttttttgaaa tttatcgaag atttaacgcc ctcccaccgt gataggagta
     5761 agcaaggtta tttaactcct tgcgatggaa agagggtgaa atcttatggt tgatgaaaag
     5821 acaattgaac atcaattcga cagtttctgt aaaacagttt tgcgtaacta tgctagagat
     5881 atttatgacg aaaataaacg cagaaatgat tatttggtat ctctcgaatc tttgtcactt
     5941 gcagaattaa gtaaattgag tattttagat gattatgatt ctaactacat ctgtatggtt
     6001 tcttacgact acaacatacg aattgaagat gtattaatgg cacaagcaat cggaaaactt
     6061 acaaaaagaa aacaagatat aattctactt tcattctttc tcaacatgac aaatgctgac
     6121 atagcaacat tgatggattt agcagaaaat accatacatt atcacaaaac aaatgcacta
     6181 aaagagttga aagaactcat ggaggaacat tgaaatgaaa cctactaaag aagtattacc
     6241 ttttacaatt attttacttg ctgtacatgg cgatgtaaat gcaataaacg gaatacttaa
     6301 acattttgaa cactatatca ttcgtctttc acagaaaact ttatttgatg aatacggaaa
     6361 tccctatatt catgtagaag aagaaacaaa gcgaatgcta ggaacaaaac taatcactgc
     6421 tattttgaga tttaatttgt cttaatctcg ccctctctcc atgtatcaag ctatttggta
     6481 catggagaat cactaattat ttgtctataa aagcccataa gctattgtga ttttttatag
     6541 acaaatcaat gccgactgtt ctttgacaac tgaataaagt agatagatac gtttgtgata
     6601 cagatagcca acaaatacta tacgccatga ccttaatcaa gagagctact atatagtgat
     6661 ttgtgaataa cctttggaac ggttgttttg ccgtaacctg taccacagta taatgataca
     6721 cctgtatgac acggttctgc ccataagaat gggattggtt aaaataccaa tggagcttac
     6781 caaagccatc tatctacttc tgaactacga acatagaaag gagtgataca ttctatgaac
     6841 aatactgatg tacctatttg ggaaaaatat accttgacga ttgaagaagc gtcaaaatac
     6901 tttcgtattg gagagaaaaa attacgtaaa cttgctgatg aaaaccttga ttctggctgg
     6961 gtcattatga atagcaatcg cattcaaata aagagaaaac aatttgagaa aatcgttgat
     7021 acacttgact ttatctaatg aaattgagcc ttatatgtta taatattcat atgacgtatc
     7081 aagctcaatt cggttattga gaggagcttc ttatcatgtc aactgaaaaa agacgagata
     7141 ataaagatag aattttaaga actggagaga gccagagaaa agatggaaga tacgcatata
     7201 agtatgtata ttcatttggt aaaccacaat ttgtttactc ttggaaactt gtacctactg
     7261 ataaaccccc aaagggaaaa cgtgatgata tatcattaag ggataaggaa aaagcaatac
     7321 aaaatgacct tgatgatagc attgacacta ttggtaagaa aatgacagtt tgtcagttat
     7381 acgctaaaaa gaatggacta agaaagaata tccgacttaa cactaaaaaa ggacgccatt
     7441 atcttatgaa gattcttaac aaggagtgat tctgtgaaga gatgtattgt agtagtggaa
     7501 tagtcaaaaa cataatatat attaaaggag agatttaaat atgactattc caagttcaaa
     7561 taaggtatat ccaagaagta atgattatca aactgtatat cttaaaaatg taataacaag
     7621 ggaaaatata gaagttggag attacacaat atataatgac ttttataatg acccaagaga
     7681 ttttgagaaa aacaatgtat tgtatcatta ccctataaac aatgataaat taataattgg
     7741 taagttttgc tcaatagcat gtaatgcaaa atttctaatg actagtggaa atcactcaat
     7801 gaaatcaaaa tccacatata cttttcccat cttttatgaa gaatggaaac tcgatatcaa
     7861 ccatataacg gacgcttggg acaataaggg taacattata attggaaatg atgtgtggat
     7921 aggttatgat tctataatta tgtcaggtgt aaatattggt gatggtgcaa ttattggaac
     7981 aagagcagta gttactaatg acgttccacc gtattctatt gttggaggag tacctgcaaa
     8041 aattataaaa aagagattta ataatgatac aatttcaaaa ttattagaaa ttaaatggtg
     8101 ggattggcca tataaaaaaa ttcaatcaaa cattaagcat attcaatcag gaaatattga
     8161 taaattagtt taatatttta taatttattt atattaaact ttactttaat aatcatttta
     8221 taagagcttt ttcaaaattt gtatattatt ttaatatata taaatttaat acaacattaa
     8281 ataaaataaa aagtaaagtt acttaataaa aagttacttt actttttatt atttaaattc
     8341 ctgtttttat tataataggt aaaattctat taaactagtt cacattttca ttattattga
     8401 tatattatat atgaatatac cattttgttt tttaataaaa aaatcatgct acatcaatag
     8461 taagcaacta ttaatgtagc atgatttttt gtatctaaat aatcttagta tttacctatc
     8521 aatatagatt gatatatcaa aaactatagt cttctatatt cttctctttt tagcttttgg
     8581 ttacttagat agtaatcaat ttcatcaatt agacgttgtt tatcatcagg taatacacct
     8641 tttaaattag cataatttga tgcatgatta cttctaaata caattttttc attagcatct
     8701 atattcttta tcatctcttt aatttcttgt aacacagcct tatcctctag tattgtaaac
     8761 tctcctgcct taagtaaatc gtaaagttca gtattaggtt ctaccattaa actaagtaca
     8821 cccacataat ctggtgaaat tgctcctaac atttttcctg tatcaactgc atgctgatgt
     8881 gtttttttag ttcctccaag acctgctatt acagtggctg ataatttaat cccacttttt
     8941 ttaacttttc taccaacttt tattaattgt tcactactaa aacctttctt aatatcttct
     9001 aatacttcat tgcttccact ttcaagccct agataaatca ttttaacacc aagttttctt
     9061 aattctttaa gttcatcatc agattttttc tctatagatt taggagagcc gtacatagta
     9121 attctttcac actctggaaa aacttctttt atatataaga taatatctct taatttctcc
     9181 attggtatta tcaatgcatc tccatctgct aagaatattc tttctacatg cttataatat
     9241 cttctaaata tatcaatttc attttttatt acttctagtg gctttatttt aaatttttta
     9301 gatttgtaca tactacaaaa cgtacatttg ttatgagaac atcctagtgt gacttgtatt
     9361 ataagactat aagcctcact tggtggtctg taaagtggca tatcgtattc catattaaaa
     9421 tcccccttgt aataaaatat tcattctatt atattattaa tatttacttt aaataataac
     9481 ttataaaatt atatttttta aattttagtt taagatatat tctttaaaaa tttaatgata
     9541 ctattggcag taatattaag ttgtttttct tgacttataa tagcatcatt atctcccttt
     9601 tgttttccat aatctccaaa ttgtgcatga tttcctcctt caatctcaac ataagttgta
     9661 tcatcaggaa gtttttgttt tgattctaca agacttttaa aattgactac tccatctttg
     9721 ctcccccata tagatataac tttttttcct aattctttca actcatcacc cattggataa
     9781 gaagctaaaa atacaacgcc ttcaattaat ttattatcac gtgcaaaatt tgatgctgca
     9841 actcctccta atgaatgacc tcctattacc caatgttcta tgttgttgta agaatccata
     9901 actttctgtg ccttattagt tccaagtata gctagattaa gtggcatctt tacgattaca
     9961 acctcatatc cactttctgc tatttttctt gcaagtggag cataagattc tatttctact
    10021 ttagctcctg gatacaaaat taaaccttta gaaacttttc ttccttttgg tgtaaatgaa
    10081 atgttttcat ttacagttac ttcaacttta ctattgctga ctagagcttc ttttgccagc
    10141 ttttgaggtt catatgtttt atttatccat atgaaaaagc caagcaaaat caatacaaat
    10201 agtataccta ttcccttaaa tatcttattc tttatgttaa attttctatt attattttta
    10261 tcatctttgt tctcaacctc atcataaagt aaaggtctct caagttccat agttatacta
    10321 aatgtatcat atttcttatt acgctttacc tttacatctt tactcataaa tctaaccccc
    10381 ataatatttg aatactatta tagtttatcc taagtaaatt gttttttcaa ccaatgaaaa
    10441 aaatcaatat tttaactatt ttcatataaa attcataaat atatgttttt tgttttttat
    10501 atattatttt cttttagtat tttataaaac aaatagccat atactttatt ttaaatatat
    10561 ggctattcta taatttaata tctaagctgc ttcttttatt gaaactttag ttatattact
    10621 tattctatac atttctttga caccatttag agtttgcagt aagtttagac atattatctt
    10681 gtcacctaga cttttagtat ttacaatact ctctaaaatt acacttcttc cttcatcata
    10741 cttatctaca ttgtaatttg aaatataatt ggtatctctc aatatttttt
//
LOCUS       DAEPIN010000214         4747 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.guided.150, whole genome shotgun sequence.
ACCESSION   DAEPIN010000214 DAEPIN010000000
VERSION     DAEPIN010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4747
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.guided.150"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            37..990
                     /gene="blaCDD"
                     /locus_tag="KR250_003889"
     CDS             37..990
                     /gene="blaCDD"
                     /locus_tag="KR250_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDD family class D beta-lactamase"
                     /protein_id="HBH0069739.1"
                     /translation="MKRKKNFIWIAILLVGVVMVMYYVGKKHNDINQKTDKNYIKSEL
                     NKSELNKSKIENNDKNKKEKINMVDYSDCFEGISGGAIFYNTKNKEYNIYNKELIETR
                     RSPCSTFKIVSTLIGLEKGVINSKESVMGYDGTEYPNKNWNKNLSLEEAFKESCVWYY
                     KKLIDKVDAKSVQNILDDLKYGNCDISEWEGDLKNGKGHLNGFWLESSLQISPKEQVQ
                     TMAKIFEGDTNFKKEHINILRDIMKIDINDKNINVYGKTGTGFDEKNKRVDAWFVGML
                     EREGDTYYFAIKSDDSNKEITGPKVKEIAINIIKKYYSVRE"
     gene            1334..2017
                     /locus_tag="KR250_003890"
     CDS             1334..2017
                     /locus_tag="KR250_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0069740.1"
                     /translation="MEILKCENLTKIYGSNQTRVTALNNVNLSVQKGDFVSIVGASGS
                     GKSTLLHLLGGVDRPTSGKIYVEDTEISSLKEEALAVFRRRKVGLIYQFYNLIPTLDV
                     RKNILLPMLLDKRKVDEDRFSEIVSILGLSDRLNHLPSQLSGGQQQRVSIARSLIYRP
                     AILLADEPTGNLDRKNSEEIVDLLNLSNKRFNQTILLITHDEKIALEANRIVTMEDGV
                     IVSEKVVKK"
     gene            2014..4674
                     /locus_tag="KR250_003891"
     CDS             2014..4674
                     /locus_tag="KR250_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0069741.1"
                     /translation="MNILQEYTLDFVRRNKRSSIAIMLAILLTTTMMSSLCGLLYTMW
                     TDFIRLSIEKEGNWHGELFDYTYGSDLPLIENFSSVDDVMVKGQWYVGKINDEKRDYI
                     IYRNATSEYWNSMPEKDTIIEGRRPNNANEIALSKQYFENNPSVKIGDKITLPIGYRI
                     LNGKPLEPIAPKSKGESFKKISEATLTIVGKVDVSTSSVIPAYTALGYLDKTSIKPND
                     SITVYLRFKNIRDTYKELPKLAKSLGWKTNEYGKYNLKYNSNYLLKMLVFSPEQKASM
                     SSISKFSTPIMYLTIAIFTVAVFVMVIYNAFSLSANARLTQLGILSSVGASPKQIKRS
                     VVFEGFLLTIIPLPIGLFLGWLLCNRLIVYVNSVNYHTDVPEVVFTYGIPAFLPAVLL
                     TIVTVWISALIPARKVSKISPIEAIRQGDSVKIKKSHKYSLGKIFGIEGELASNALRA
                     RKKSYRTATISLTLSFLLLTCFLHIIANQEGAKAVYGSDNYKDQRDIAVYLENSEPIE
                     SAFIEKLDNTEGVKSSLYYQKMSASTWLTEKDASKELAKNGGFKSIVSSKKYSPIERD
                     GKFRIISNIIGLDDKSFTEYCRQISVDPKLFYNTKNPTSIVYNKEEDINRSTRRNKVY
                     IDYLNLSIGDNIKLNEKVYDEDKGDYTYNMKVGAITSTLPKIVDTSSKYTLMQIVPMS
                     MVQEISKNYDEVSRLRSNRLIGVFKVNNREDIPRVREKLESIYNKDYGSGDYSIEDVL
                     ESEKQKASGIKTMNLIVGFITGLLAMIGLSNVLATVSGNIRSRRQEFAMLRSVGLSPE
                     GIKKMLILEGLFLGITPLLLSIPVQIGIVYAFLRINEIYFIEYLPFAPISTIIGFTIL
                     ILFIVIASYMTGYKQLKNENIVESIKNETI"
BASE COUNT         1844 a          540 c          826 g         1537 t
ORIGIN      
        1 aggttataaa tttaaatatg ggagatgata taaattatga aaagaaaaaa gaattttatt
       61 tggatagcta tcttattggt tggtgtagtt atggtaatgt attatgtagg gaaaaaacat
      121 aacgatataa atcaaaaaac tgataagaat tatataaaat ctgaattaaa taagtctgaa
      181 ttaaataaat ccaaaattga aaacaatgat aaaaacaaaa aagaaaaaat taatatggtt
      241 gactatagtg attgttttga aggtattagt ggaggagcta ttttttataa tactaaaaat
      301 aaagaatata atatatataa caaagaatta attgaaacaa gaaggtcacc atgttctact
      361 tttaaaatag tttcaacttt aattggttta gaaaaagggg taataaactc aaaagaatca
      421 gttatgggat atgatggtac agagtatcca aataaaaatt ggaataaaaa tcttagttta
      481 gaagaggcat ttaaggagtc ttgtgtttgg tattacaaaa aattgataga taaagttgat
      541 gctaaatctg tccaaaacat tttagatgat ttaaagtatg ggaattgcga tattagcgaa
      601 tgggaaggcg atttaaaaaa tggaaaggga catttaaatg gattttggtt agaatcttca
      661 ttacaaatat ctccaaaaga acaagtgcaa acaatggcaa aaatatttga gggagataca
      721 aactttaaaa aagagcatat taatatttta agagatatta tgaaaataga tataaatgat
      781 aaaaacataa atgtatatgg aaaaactgga acaggatttg atgaaaaaaa taaacgtgtg
      841 gatgcatggt ttgttggtat gctagagcgt gaaggtgaca cctattattt tgctattaaa
      901 tcagatgatt ccaataaaga aattacaggg ccaaaggtaa aggaaattgc aattaatata
      961 attaagaaat attatagtgt aagggaataa acttaatttt atttgtaaat attatttacc
     1021 tatagtctgt aatatttatt aaataatttt actaaattga gtctatatag agcaaaactc
     1081 ctttagtaca taaaatactt gctattgatt attaaattga atacctatag atttaaagtt
     1141 aatacctttt aaagactatg taatatatta aaagtataaa taaaatatat tgcatagttt
     1201 ttgttttata ttataatttt aaataatgtg caaaaactaa acaatatttt taatagtaat
     1261 ttatatttgt attcatgtca ctataatgtc attttaggtt gctaaaattt aaccataaag
     1321 gagatgattg tatatggaaa tattaaaatg tgaaaatctc acaaaaatat atggttcaaa
     1381 tcaaaccaga gtgacagccc ttaataatgt aaatttatca gttcaaaaag gtgattttgt
     1441 atccattgta ggagcatctg gttctggcaa atctacattg ttacatctgc ttgggggagt
     1501 agatagacct acatctggaa agatttatgt tgaagataca gaaatatcaa gtttaaagga
     1561 agaagctctt gcagttttta gacgtagaaa agtaggtctt atttatcaat tttataatct
     1621 tatacctacc cttgatgtta gaaaaaatat attattaccc atgttacttg ataaaagaaa
     1681 ggtggatgag gatagattct ctgagattgt atctatattg ggattatctg atagattaaa
     1741 ccatcttcca agtcaacttt caggtggaca acaacaaagg gtatctatag ctagaagttt
     1801 aatctataga ccagctattt tattagcaga tgagccaact ggtaatcttg atagaaaaaa
     1861 ttcagaagaa attgtagact tgttaaactt atcaaataaa cgatttaatc agacaatact
     1921 tcttattact catgatgaga aaatagcact agaggcaaat cgtattgtaa ctatggaaga
     1981 tggagtgatt gtctctgaaa aggtggtgaa gaaatgaata ttcttcaaga atacacctta
     2041 gactttgtac gacgcaataa acgtagtagt atagctataa tgttagctat attacttact
     2101 acaaccatga tgtcttcttt gtgtggactg ttgtatacca tgtggacaga ttttataaga
     2161 ctttcaattg agaaagaagg taactggcat ggtgaattgt ttgactatac ttatggaagt
     2221 gacttaccat taattgaaaa tttttcttca gttgatgatg taatggtaaa aggacaatgg
     2281 tatgtaggca aaattaatga tgagaaaaga gattatataa tttatagaaa tgctacatca
     2341 gagtattgga attctatgcc agaaaaggat actataatag agggtcgtcg accaaataat
     2401 gctaatgaaa ttgcattatc aaaacaatat tttgagaata atccaagtgt aaaaattggt
     2461 gataaaatta ctctcccaat aggatataga atcttaaatg gaaagcctct tgaacccata
     2521 gccccaaaat cgaaagggga aagttttaaa aaaatttcag aggctacatt gacaatagta
     2581 ggaaaggtag atgtttctac ttcatcagta atacctgcat atacagctct tggatatctt
     2641 gataaaacaa gtataaaacc taatgatagt ataactgtgt acttgcgatt taaaaatata
     2701 agagacacgt ataaggaact tccaaaactt gctaaatcat tgggatggaa gactaatgaa
     2761 tatggaaaat acaatcttaa atacaatagt aattatcttt taaagatgtt agtcttttca
     2821 ccagaacaga aagcatctat gagtagtata tcaaaatttt caactcctat tatgtatctt
     2881 acaatagcaa tttttacagt agccgtgttt gtaatggtga tttacaacgc gttttcactg
     2941 tctgcaaatg ctagacttac tcagttaggg atattatcta gtgttggtgc atcaccaaaa
     3001 caaattaaaa ggtctgtagt gtttgaaggc tttttactga caattatacc attaccaata
     3061 ggtttatttt taggatggtt attgtgtaat aggcttatag tttatgttaa ttcagttaat
     3121 tatcatactg atgttccaga agtggtattt acttatggta ttcctgcttt tctgccagca
     3181 gttttactta caatagtaac agtctggatt tctgcactta taccagcacg aaaagtatca
     3241 aagatatctc ctatagaagc aatacgacaa ggagatagtg ttaaaatcaa aaagtcgcat
     3301 aaatattcat taggaaagat atttggaata gaaggagaac ttgcatcaaa cgcactacgt
     3361 gctcgtaaaa aatcttatcg tactgcaaca atatctttaa cattatcttt tttattactt
     3421 acatgttttt tgcatataat tgcaaatcaa gaaggagcaa aagctgttta tggaagtgat
     3481 aattataagg accaaagaga tatagcagtt tatttggaaa atagtgaacc tatagagtct
     3541 gctttcattg aaaaattaga taatacagaa ggtgtaaaaa gttcactata ttatcaaaaa
     3601 atgtctgctt ccacttggct gactgaaaaa gatgcatcaa aagaacttgc gaaaaatgga
     3661 ggatttaaaa gtatagtttc atctaaaaaa tactctccta tagaacgtga tggcaagttt
     3721 cgtataattt caaatataat tgggcttgat gataagagtt ttacagaata ttgtaggcaa
     3781 atttctgtag acccaaagtt attttataat acaaaaaatc caacctcaat tgtatataat
     3841 aaggaggaag atattaatcg tagtacacgt agaaataaag tatatataga ttatttaaat
     3901 ctttcaatag gagataacat taaattaaat gaaaaagtat atgacgaaga taaaggggat
     3961 tatacttaca acatgaaagt gggagctatt acaagcacac ttcctaaaat agtagatact
     4021 agttcaaagt atacattaat gcaaatagtt ccaatgtcta tggtacagga aatatctaaa
     4081 aattatgatg aagtaagtag gttacgttca aataggctga taggagtatt taaagtaaat
     4141 aatcgtgagg acattcctag agtaagagaa aaattagagt ccatttataa taaagactat
     4201 ggttcaggag attatagtat tgaggatgtt ttagaaagtg aaaaacaaaa ggctagtgga
     4261 ataaagacca tgaatttaat agtaggcttt attacaggtt tattggctat gattggatta
     4321 tcaaatgtac ttgctacagt gtcaggaaat attagaagta gacgacaaga gtttgctatg
     4381 ttgcgttctg ttggattatc tccagaaggc ataaaaaaga tgcttatttt ggaaggttta
     4441 ttcttaggaa ttacaccatt actacttagt attcctgtgc aaataggaat agtgtatgct
     4501 tttctacgta taaatgaaat atattttata gagtatttac catttgcacc tatatctaca
     4561 ataataggtt ttactatatt gatacttttt attgtaatag catcatatat gacaggatac
     4621 aagcagttga agaatgaaaa tatagtagaa tctataaaaa atgaaacaat ataaacaaaa
     4681 tctatttgaa cttatttaaa ggtttcgatt taagactact tacaagtata gggatagtta
     4741 agttgtt
//
LOCUS       DAEPIN010000215         2609 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.guided.179, whole genome shotgun sequence.
ACCESSION   DAEPIN010000215 DAEPIN010000000
VERSION     DAEPIN010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2609
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.guided.179"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            124..753
                     /locus_tag="KR250_003892"
     CDS             124..753
                     /locus_tag="KR250_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBH0069742.1"
                     /translation="MVDSIIFDLDGTLWDSTEEVCKVWQDVLSEHKEIELSVTKDLFR
                     SLMGLSFEEIANRLFPNLSEEERMNLLNECSLKECDYLSENGAKLFDDIEDTLKLLSK
                     KYKLFIVSNCQAGYIESFLKAHKLEQYFIDFECPGNTGLHKGENNKLIIERNKLINPI
                     YVGDTQGDANSAKFVGIPFIYAKYGFGNVDVYDYFIDSFKDLLEHDILK"
     gene            808..1464
                     /locus_tag="KR250_003893"
     CDS             808..1464
                     /locus_tag="KR250_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBH0069743.1"
                     /translation="MELQDTIFKRQSVRKFKNQDVSDEDILKMIKAAGAAPSGKNIQN
                     WHFVVIKRRDLMEKIADVITKKQQEILVEMDKVSVDKANRFRKFVKNFTLFYLKAPVL
                     VLVFTKVYNPSGYYELELIDAPKETIDKLFIRNPGMQSLGAAIENFTLSAIELGYGSC
                     WLTSQNYAADEIEAVLEAETGFEKGEYFLGAMLALGVPEDNLKSPSKKPVEEICTFIK
                     "
     gene            1879..2595
                     /locus_tag="KR250_003894"
     CDS             1879..2595
                     /locus_tag="KR250_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0069744.1"
                     /translation="MNSYNILVVEDEKEIADAIEIYLLNQGYNVFKGYNGLEGLKVIE
                     NQEIHLAIVDIMMPKMDGITLTMKLRENYNFPVIMLSAKSEEVDKIMGLNIGADDYVT
                     KPFKPLELLARVNSQLRRYTKYLNMVGNKEQKVESDDSVFAIGGLELNENTKEVSIDG
                     KYIKTTPIEFKILSLLMRNAGRVFSADEIYERVWNENAVNTDTVMVHVRNIREKIEID
                     PKNPKYLKVVWGVGYKIEKI"
BASE COUNT         1084 a          221 c          461 g          843 t
ORIGIN      
        1 ttttgacaat tggttttata ttgtagagca gatacaatca agtaaaatat atttatgatt
       61 gtatctgctt tttagtatat aattataaat atataataaa ctttgacata agggggattt
      121 tttgtggtag atagtattat ttttgattta gatggaacat tgtgggattc aactgaagaa
      181 gtatgcaaag tctggcagga tgttttaagt gagcataagg aaatagaatt aagtgtcaca
      241 aaagatttat ttagaagtct tatggggctt tcttttgagg aaatagcaaa tcgtttattt
      301 ccaaatttaa gtgaagaaga aagaatgaat ttattgaatg aatgttcttt gaaagaatgt
      361 gactatttat ctgaaaatgg agctaaatta tttgatgata tagaagatac tcttaaatta
      421 ctatctaaaa aatataaatt atttatagta agtaattgtc aagctggata tatagaatct
      481 tttttaaaag cacataaact agaacagtat tttatagatt ttgaatgtcc tggtaatact
      541 ggtttgcata aaggagaaaa taataaactt ataattgaaa gaaataaatt aataaatcca
      601 atctatgttg gtgatacgca aggtgatgca aatagtgcta agtttgtagg tatacctttt
      661 atatatgcca aatatgggtt tgggaatgta gatgtatatg attattttat agattcattt
      721 aaagatttgt tagaacatga tatattaaaa taagatgagt ttaaaaatta tattaaaata
      781 ataaaaaagt tatgggggga tttaaaaatg gaattacaag atactatttt taaaagacaa
      841 agtgtaagaa agtttaaaaa tcaagatgta tcagatgaag atattttaaa aatgataaaa
      901 gcagcaggtg cagcaccatc aggcaagaat attcaaaact ggcattttgt tgttataaaa
      961 cgccgtgatt taatggaaaa gatagcagat gtgataacta aaaagcaaca agaaatactt
     1021 gtagaaatgg ataaagtatc agtggataag gctaatagat ttagaaaatt tgttaaaaat
     1081 tttacactat tttacttaaa agctccagtt ttagtattag tttttacaaa ggtatataat
     1141 ccatcaggat attatgaatt agagcttata gatgcaccta aggagactat agataaatta
     1201 tttataagaa atccaggtat gcagagtcta ggtgctgcaa ttgaaaactt tacactatca
     1261 gcaattgaac ttggatatgg ttcttgttgg ttgacaagtc aaaactatgc agcagatgag
     1321 atagaagctg ttttagaagc agaaactggt tttgaaaagg gagagtattt tttaggtgca
     1381 atgttagctc ttggagtgcc agaggacaat ttgaagagtc catctaaaaa accagtagaa
     1441 gaaatatgta catttataaa ataaaagtca aggacatttt aaaatcgaag taaattaaaa
     1501 acaaaataaa ttaaaaataa atatttaaaa aattaaaaag taggttttat tgatattaat
     1561 tttatatttc aaatcattaa tttaattaat acaataagaa ctactttttt ttgtatatta
     1621 attttaaata tatttaaatc tttagaaaat ctttagaaaa gtatatgtaa tatcttaagt
     1681 ttttaatttt aatattaagt atataaaaca aaatgaaaaa atattaaatt taaactagaa
     1741 ggaggtttct aagatggaca ttaaatacaa ataaccagaa caaaagatat aaatatatat
     1801 ataaatggaa aaacttctat catattgtat aatcatatat gtaaagaaaa tttataaatt
     1861 aatataaagg agattagaat gaattcatat aatattttgg tggtagaaga tgaaaaggag
     1921 atagcagatg ctatagaaat atatctatta aatcaaggat ataatgtttt taagggatac
     1981 aatggattag aaggtcttaa ggttatagaa aaccaagaaa tacatttagc tatagttgat
     2041 ataatgatgc ctaaaatgga tggtataaca cttacaatga agcttagaga aaactataat
     2101 tttccagtaa taatgctatc tgcaaaatca gaagaagttg acaaaataat ggggcttaat
     2161 ataggagcag acgattatgt aacaaaacct tttaagccat tagaactatt ggctagagtt
     2221 aattcacaac ttagaaggta cactaagtat ctaaacatgg ttggaaataa agagcaaaag
     2281 gtagaaagtg acgatagtgt gtttgcaata ggcggattag aattaaatga aaatacaaaa
     2341 gaagttagta ttgatggaaa gtatataaaa acaactccta tagaatttaa aatactaagt
     2401 ttacttatga gaaatgcagg aagagttttt tctgctgatg aaatatatga aagagtttgg
     2461 aatgagaatg ctgtaaatac agatacagtt atggtacatg tgaggaatat aagggaaaag
     2521 atagaaattg acccaaaaaa tcctaaatat ttaaaggtgg tgtggggtgt tggatacaaa
     2581 attgaaaaaa tttaaagata aatatatag
//
LOCUS       DAEPIN010000216         2609 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 23B09CD0010
            SAMN05941528-rid12469653.guided.180, whole genome shotgun sequence.
ACCESSION   DAEPIN010000216 DAEPIN010000000
VERSION     DAEPIN010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05941528
            Sequence Read Archive: SRR6243579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068140.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068140.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/13/2021 00:51:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,829
            Genes (coding)                    :: 3,772
            CDSs (with protein)               :: 3,772
            Genes (RNA)                       :: 68
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 22 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2609
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05941528-rid12469653.guided.180"
                     /strain="23B09CD0010"
                     /isolation_source="stool sample (feces)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Canada"
                     /collection_date="2009"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            124..753
                     /locus_tag="KR250_003895"
     CDS             124..753
                     /locus_tag="KR250_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBH0069745.1"
                     /translation="MVDSIIFDLDGTLWDSTEEVCKVWQDVLSEHKEIELSVTKDLFR
                     SLMGLSFEEIANRLFPNLSEEERMNLLNECSLKECDYLSENGAKLFDDIEDTLKLLSK
                     KYKLFIVSNCQAGYIESFLKAHKLEQYFIDFECPGNTGLHKGENNKLIIERNKLINPI
                     YVGDTQGDANSAKFVGIPFIYAKYGFGNVDVYDYFIDSFKDLLEHDILK"
     gene            808..1464
                     /locus_tag="KR250_003896"
     CDS             808..1464
                     /locus_tag="KR250_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBH0069746.1"
                     /translation="MELQDTIFKRQSVRKFKNQDVSDEDILKMIKAAGAAPSGKNIQN
                     WHFVVIKRRDLMEKIADVITKKQQEILVEMDKVSVDKANRFRKFVKNFTLFYLKAPVL
                     VLVFTKVYNPSGYYELELIDAPKETIDKLFIRNPGMQSLGAAIENFTLSAIELGYGSC
                     WLTSQNYAADEIEAVLEAETGFEKGEYFLGAMLALGVPEDNLKSPSKKPVEEICTFIK
                     "
     gene            1879..2595
                     /locus_tag="KR250_003897"
     CDS             1879..2595
                     /locus_tag="KR250_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0069747.1"
                     /translation="MNSYNILVVEDEKEIADAIEIYLLNQGYNVFKGYNGLEGLKVIE
                     NQEIHLAIVDIMMPKMDGITLTMKLRENYNFPVIMLSAKSEEVDKIMGLNIGADDYVT
                     KPFKPLELLARVNSQLRRYTKYLNMVGNKEQKVESDDSVFAIGGLELNENTKEVSIDG
                     KYIKTTPIEFKILSLLMRNAGRVFSADEIYERVWNENAVNTDTVMVHVRNIREKIEID
                     PKNPKYLKVVWGFGYKIEKI"
BASE COUNT         1084 a          221 c          460 g          844 t
ORIGIN      
        1 ttttgacaat tggttttata ttgtagagca gatacaatca agtaaaatat atttatgatt
       61 gtatctgctt tttagtatat aattataaat atataataaa ctttgacata agggggattt
      121 tttgtggtag atagtattat ttttgattta gatggaacat tgtgggattc aactgaagaa
      181 gtatgcaaag tctggcagga tgttttaagt gagcataagg aaatagaatt aagtgtcaca
      241 aaagatttat ttagaagtct tatggggctt tcttttgagg aaatagcaaa tcgtttattt
      301 ccaaatttaa gtgaagaaga aagaatgaat ttattgaatg aatgttcttt gaaagaatgt
      361 gactatttat ctgaaaatgg agctaaatta tttgatgata tagaagatac tcttaaatta
      421 ctatctaaaa aatataaatt atttatagta agtaattgtc aagctggata tatagaatct
      481 tttttaaaag cacataaact agaacagtat tttatagatt ttgaatgtcc tggtaatact
      541 ggtttgcata aaggagaaaa taataaactt ataattgaaa gaaataaatt aataaatcca
      601 atctatgttg gtgatacgca aggtgatgca aatagtgcta agtttgtagg tatacctttt
      661 atatatgcca aatatgggtt tgggaatgta gatgtatatg attattttat agattcattt
      721 aaagatttgt tagaacatga tatattaaaa taagatgagt ttaaaaatta tattaaaata
      781 ataaaaaagt tatgggggga tttaaaaatg gaattacaag atactatttt taaaagacaa
      841 agtgtaagaa agtttaaaaa tcaagatgta tcagatgaag atattttaaa aatgataaaa
      901 gcagcaggtg cagcaccatc aggcaagaat attcaaaact ggcattttgt tgttataaaa
      961 cgccgtgatt taatggaaaa gatagcagat gtgataacta aaaagcaaca agaaatactt
     1021 gtagaaatgg ataaagtatc agtggataag gctaatagat ttagaaaatt tgttaaaaat
     1081 tttacactat tttacttaaa agctccagtt ttagtattag tttttacaaa ggtatataat
     1141 ccatcaggat attatgaatt agagcttata gatgcaccta aggagactat agataaatta
     1201 tttataagaa atccaggtat gcagagtcta ggtgctgcaa ttgaaaactt tacactatca
     1261 gcaattgaac ttggatatgg ttcttgttgg ttgacaagtc aaaactatgc agcagatgag
     1321 atagaagctg ttttagaagc agaaactggt tttgaaaagg gagagtattt tttaggtgca
     1381 atgttagctc ttggagtgcc agaggacaat ttgaagagtc catctaaaaa accagtagaa
     1441 gaaatatgta catttataaa ataaaagtca aggacatttt aaaatcgaag taaattaaaa
     1501 acaaaataaa ttaaaaataa atatttaaaa aattaaaaag taggttttat tgatattaat
     1561 tttatatttc aaatcattaa tttaattaat acaataagaa ctactttttt ttgtatatta
     1621 attttaaata tatttaaatc tttagaaaat ctttagaaaa gtatatgtaa tatcttaagt
     1681 ttttaatttt aatattaagt atataaaaca aaatgaaaaa atattaaatt taaactagaa
     1741 ggaggtttct aagatggaca ttaaatacaa ataaccagaa caaaagatat aaatatatat
     1801 ataaatggaa aaacttctat catattgtat aatcatatat gtaaagaaaa tttataaatt
     1861 aatataaagg agattagaat gaattcatat aatattttgg tggtagaaga tgaaaaggag
     1921 atagcagatg ctatagaaat atatctatta aatcaaggat ataatgtttt taagggatac
     1981 aatggattag aaggtcttaa ggttatagaa aaccaagaaa tacatttagc tatagttgat
     2041 ataatgatgc ctaaaatgga tggtataaca cttacaatga agcttagaga aaactataat
     2101 tttccagtaa taatgctatc tgcaaaatca gaagaagttg acaaaataat ggggcttaat
     2161 ataggagcag acgattatgt aacaaaacct tttaagccat tagaactatt ggctagagtt
     2221 aattcacaac ttagaaggta cactaagtat ctaaacatgg ttggaaataa agagcaaaag
     2281 gtagaaagtg acgatagtgt gtttgcaata ggcggattag aattaaatga aaatacaaaa
     2341 gaagttagta ttgatggaaa gtatataaaa acaactccta tagaatttaa aatactaagt
     2401 ttacttatga gaaatgcagg aagagttttt tctgctgatg aaatatatga aagagtttgg
     2461 aatgagaatg ctgtaaatac agatacagtt atggtacatg tgaggaatat aagggaaaag
     2521 atagaaattg acccaaaaaa tcctaaatat ttaaaggtgg tgtggggttt tggatacaaa
     2581 attgaaaaaa tttaaagata aatatatag
//
